LOCUS       DAZFAB010000001       122477 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZFAB010000001 DAZFAB010000000
VERSION     DAZFAB010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 122477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 122477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..122477
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.001"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            184..2427
                     /gene="pdeF"
                     /locus_tag="ACHJWA_000001"
     CDS             184..2427
                     /gene="pdeF"
                     /locus_tag="ACHJWA_000001"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFO4306697.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDVKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRTVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIGPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLDKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(2466..4007)
                     /gene="ppx"
                     /locus_tag="ACHJWA_000002"
     CDS             complement(2466..4007)
                     /gene="ppx"
                     /locus_tag="ACHJWA_000002"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFO4306698.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(4012..6078)
                     /gene="ppk1"
                     /locus_tag="ACHJWA_000003"
     CDS             complement(4012..6078)
                     /gene="ppk1"
                     /locus_tag="ACHJWA_000003"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFO4306699.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(6249..6887)
                     /gene="purN"
                     /locus_tag="ACHJWA_000004"
     CDS             complement(6249..6887)
                     /gene="purN"
                     /locus_tag="ACHJWA_000004"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFO4306700.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHPHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDVTARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(6887..7924)
                     /gene="purM"
                     /locus_tag="ACHJWA_000005"
     CDS             complement(6887..7924)
                     /gene="purM"
                     /locus_tag="ACHJWA_000005"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFO4306701.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVNAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            8249..8875
                     /gene="upp"
                     /locus_tag="ACHJWA_000006"
     CDS             8249..8875
                     /gene="upp"
                     /locus_tag="ACHJWA_000006"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFO4306702.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            8961..10250
                     /gene="uraA"
                     /locus_tag="ACHJWA_000007"
     CDS             8961..10250
                     /gene="uraA"
                     /locus_tag="ACHJWA_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFO4306703.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            10345..11046
                     /gene="hda"
                     /locus_tag="ACHJWA_000008"
     CDS             10345..11046
                     /gene="hda"
                     /locus_tag="ACHJWA_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFO4306704.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(11184..11543)
                     /gene="arsC"
                     /locus_tag="ACHJWA_000009"
     CDS             complement(11184..11543)
                     /gene="arsC"
                     /locus_tag="ACHJWA_000009"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFO4306705.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(11564..13027)
                     /gene="bepA"
                     /locus_tag="ACHJWA_000010"
     CDS             complement(11564..13027)
                     /gene="bepA"
                     /locus_tag="ACHJWA_000010"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFO4306706.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRNAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            13240..14301
                     /gene="yfgO"
                     /locus_tag="ACHJWA_000011"
     CDS             13240..14301
                     /gene="yfgO"
                     /locus_tag="ACHJWA_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO4306707.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(14339..15187)
                     /gene="focB"
                     /locus_tag="ACHJWA_000012"
     CDS             complement(14339..15187)
                     /gene="focB"
                     /locus_tag="ACHJWA_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HFO4306708.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPTSFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSICYRAI
                     YLRQES"
     gene            complement(15209..17221)
                     /gene="hyfR"
                     /locus_tag="ACHJWA_000013"
     CDS             complement(15209..17221)
                     /gene="hyfR"
                     /locus_tag="ACHJWA_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HFO4306709.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLHLLASNGCESSLLIPLTFGN
                     HTPGALLLAHTSTTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRAIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(17251..17664)
                     /gene="hyfJ"
                     /locus_tag="ACHJWA_000014"
     CDS             complement(17251..17664)
                     /gene="hyfJ"
                     /locus_tag="ACHJWA_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFO4306710.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVTFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            complement(17657..18415)
                     /gene="hyfI"
                     /locus_tag="ACHJWA_000015"
     CDS             complement(17657..18415)
                     /gene="hyfI"
                     /locus_tag="ACHJWA_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HFO4306711.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKRHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGQEICDRLLRHLSND
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(18412..18957)
                     /gene="hyfH"
                     /locus_tag="ACHJWA_000016"
     CDS             complement(18412..18957)
                     /gene="hyfH"
                     /locus_tag="ACHJWA_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFO4306712.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPTNALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTN
                     KADLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLRAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            complement(18967..20682)
                     /gene="hyfG"
                     /locus_tag="ACHJWA_000017"
     CDS             complement(18967..20682)
                     /gene="hyfG"
                     /locus_tag="ACHJWA_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HFO4306713.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVQYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYPFINEGNSGARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(20672..22252)
                     /gene="hyfF"
                     /locus_tag="ACHJWA_000018"
     CDS             complement(20672..22252)
                     /gene="hyfF"
                     /locus_tag="ACHJWA_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFO4306714.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGHSATRTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     EHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLEAAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGRP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(22257..22907)
                     /gene="hyfE"
                     /locus_tag="ACHJWA_000019"
     CDS             complement(22257..22907)
                     /gene="hyfE"
                     /locus_tag="ACHJWA_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFO4306715.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(22919..24358)
                     /gene="hyfD"
                     /locus_tag="ACHJWA_000020"
     CDS             complement(22919..24358)
                     /gene="hyfD"
                     /locus_tag="ACHJWA_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HFO4306716.1"
                     /translation="MENLALTTLLLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVCTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWVLISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFALSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            complement(24375..25322)
                     /gene="hyfC"
                     /locus_tag="ACHJWA_000021"
     CDS             complement(24375..25322)
                     /gene="hyfC"
                     /locus_tag="ACHJWA_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFO4306717.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPISSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGCAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(25333..27351)
                     /gene="hyfB"
                     /locus_tag="ACHJWA_000022"
     CDS             complement(25333..27351)
                     /gene="hyfB"
                     /locus_tag="ACHJWA_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HFO4306718.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHEGVTLVARFATPFDFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNIFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISALPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTQVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(27351..27968)
                     /gene="hyfA"
                     /locus_tag="ACHJWA_000023"
     CDS             complement(27351..27968)
                     /gene="hyfA"
                     /locus_tag="ACHJWA_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFO4306719.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCVQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENAPTQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGREDPLSLTQEQR"
     gene            complement(28221..28691)
                     /gene="bcp"
                     /locus_tag="ACHJWA_000024"
     CDS             complement(28221..28691)
                     /gene="bcp"
                     /locus_tag="ACHJWA_000024"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFO4306720.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(28691..29263)
                     /gene="gcvR"
                     /locus_tag="ACHJWA_000025"
     CDS             complement(28691..29263)
                     /gene="gcvR"
                     /locus_tag="ACHJWA_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFO4306721.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            29409..30287
                     /gene="dapA"
                     /locus_tag="ACHJWA_000026"
     CDS             29409..30287
                     /gene="dapA"
                     /locus_tag="ACHJWA_000026"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFO4306722.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            30304..31338
                     /gene="bamC"
                     /locus_tag="ACHJWA_000027"
     CDS             30304..31338
                     /gene="bamC"
                     /locus_tag="ACHJWA_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFO4306723.1"
                     /translation="MAYSVHKSRLATVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            31551..32264
                     /gene="purC"
                     /locus_tag="ACHJWA_000028"
     CDS             31551..32264
                     /gene="purC"
                     /locus_tag="ACHJWA_000028"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFO4306724.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            32432..33295
                     /gene="ypfJ"
                     /locus_tag="ACHJWA_000029"
     CDS             32432..33295
                     /gene="ypfJ"
                     /locus_tag="ACHJWA_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFO4306725.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            33310..35325
                     /gene="tmcA"
                     /locus_tag="ACHJWA_000030"
     CDS             33310..35325
                     /gene="tmcA"
                     /locus_tag="ACHJWA_000030"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFO4306726.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHFKRVLTADNDAIFWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQILQQLLTMPSGVAAVTAARGRGKSALAGQLISRIAG
                     SAIITAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGVGQQLIASALQYRPGLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNNAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNVSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNDVRTERLRDRITQWQFFH"
     gene            35399..36097
                     /gene="ypfH"
                     /locus_tag="ACHJWA_000031"
     CDS             35399..36097
                     /gene="ypfH"
                     /locus_tag="ACHJWA_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO4306727.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(36178..36378)
                     /gene="ypfN"
                     /locus_tag="ACHJWA_000032"
     CDS             complement(36178..36378)
                     /gene="ypfN"
                     /locus_tag="ACHJWA_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFO4306728.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(36406..37533)
                     /gene="dapE"
                     /locus_tag="ACHJWA_000033"
     CDS             complement(36406..37533)
                     /gene="dapE"
                     /locus_tag="ACHJWA_000033"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFO4306729.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKAVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(37537..37893)
                     /gene="yffB"
                     /locus_tag="ACHJWA_000034"
     CDS             complement(37537..37893)
                     /gene="yffB"
                     /locus_tag="ACHJWA_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFO4306730.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNIITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQKFFHEV"
     gene            38274..38333
                     /gene="ypfM"
                     /locus_tag="ACHJWA_000035"
     CDS             38274..38333
                     /gene="ypfM"
                     /locus_tag="ACHJWA_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFO4306731.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(38432..41545)
                     /gene="acrD"
                     /locus_tag="ACHJWA_000036"
     CDS             complement(38432..41545)
                     /gene="acrD"
                     /locus_tag="ACHJWA_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFO4306732.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATAEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(41709..43409)
                     /gene="narQ"
                     /locus_tag="ACHJWA_000037"
     CDS             complement(41709..43409)
                     /gene="narQ"
                     /locus_tag="ACHJWA_000037"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFO4306733.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGTPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLGRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYRSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            43615..45594
                     /gene="aegA"
                     /locus_tag="ACHJWA_000038"
     CDS             43615..45594
                     /gene="aegA"
                     /locus_tag="ACHJWA_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFO4306734.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDAALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHLTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLIRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            45662..46237
                     /gene="nudK"
                     /locus_tag="ACHJWA_000039"
     CDS             45662..46237
                     /gene="nudK"
                     /locus_tag="ACHJWA_000039"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFO4306735.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLID"
     gene            46363..47406
                     /gene="ypfG"
                     /locus_tag="ACHJWA_000040"
     CDS             46363..47406
                     /gene="ypfG"
                     /locus_tag="ACHJWA_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFO4306736.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSDLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVVVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(47501..49504)
                     /gene="tkt"
                     /locus_tag="ACHJWA_000041"
     CDS             complement(47501..49504)
                     /gene="tkt"
                     /locus_tag="ACHJWA_000041"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO4306737.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKDIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(49524..50474)
                     /gene="tal"
                     /locus_tag="ACHJWA_000042"
     CDS             complement(49524..50474)
                     /gene="tal"
                     /locus_tag="ACHJWA_000042"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO4306738.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            50763..53042
                     /gene="maeB"
                     /locus_tag="ACHJWA_000043"
     CDS             50763..53042
                     /gene="maeB"
                     /locus_tag="ACHJWA_000043"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFO4306739.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            53335..53670
                     /gene="eutS"
                     /locus_tag="ACHJWA_000044"
     CDS             53335..53670
                     /gene="eutS"
                     /locus_tag="ACHJWA_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFO4306740.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            53683..54162
                     /gene="eutP"
                     /locus_tag="ACHJWA_000045"
     CDS             53683..54162
                     /gene="eutP"
                     /locus_tag="ACHJWA_000045"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFO4306741.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            54137..54838
                     /gene="eutQ"
                     /locus_tag="ACHJWA_000046"
     CDS             54137..54838
                     /gene="eutQ"
                     /locus_tag="ACHJWA_000046"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFO4306742.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDELIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAQPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            54835..55566
                     /gene="eutT"
                     /locus_tag="ACHJWA_000047"
     CDS             54835..55566
                     /gene="eutT"
                     /locus_tag="ACHJWA_000047"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFO4306743.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPLGCQAIVGLSDEDLHRLSHQPLRYLGH
                     DHLVPEASHGRDAALLNLLRTKVREAETVAAQVFITRSFEVLRPDILQALNRLSSTVY
                     VMMILSVTKQPLTVKQIQQRLGETQ"
     gene            55563..56579
                     /gene="pta"
                     /locus_tag="ACHJWA_000048"
     CDS             55563..56579
                     /gene="pta"
                     /locus_tag="ACHJWA_000048"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO4306744.1"
                     /translation="MIIERCCELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            56618..56911
                     /gene="eutM"
                     /locus_tag="ACHJWA_000049"
     CDS             56618..56911
                     /gene="eutM"
                     /locus_tag="ACHJWA_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFO4306745.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            57018..57305
                     /gene="eutN"
                     /locus_tag="ACHJWA_000050"
     CDS             57018..57305
                     /gene="eutN"
                     /locus_tag="ACHJWA_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFO4306746.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPHGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            57317..58720
                     /gene="eutE"
                     /locus_tag="ACHJWA_000051"
     CDS             57317..58720
                     /gene="eutE"
                     /locus_tag="ACHJWA_000051"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFO4306747.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     IAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAGKIA
                     AAIGLKVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            58731..59567
                     /gene="eutJ"
                     /locus_tag="ACHJWA_000052"
     CDS             58731..59567
                     /gene="eutJ"
                     /locus_tag="ACHJWA_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFO4306748.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTFAGNRRISLEEAEQYKRGHGEEIWPTVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            59557..60744
                     /gene="eutG"
                     /locus_tag="ACHJWA_000053"
     CDS             59557..60744
                     /gene="eutG"
                     /locus_tag="ACHJWA_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFO4306749.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQLVGLYA
                     AAQ"
     gene            60844..62070
                     /gene="eutH"
                     /locus_tag="ACHJWA_000054"
     CDS             60844..62070
                     /gene="eutH"
                     /locus_tag="ACHJWA_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFO4306750.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKDDATAAKTEAEAQS"
     gene            62067..63470
                     /gene="eutA"
                     /locus_tag="ACHJWA_000055"
     CDS             62067..63470
                     /gene="eutA"
                     /locus_tag="ACHJWA_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFO4306751.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKSLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDVRSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALIQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            63482..64843
                     /gene="eutB"
                     /locus_tag="ACHJWA_000056"
     CDS             63482..64843
                     /gene="eutB"
                     /locus_tag="ACHJWA_000056"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HFO4306752.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            64864..65751
                     /gene="eutC"
                     /locus_tag="ACHJWA_000057"
     CDS             64864..65751
                     /gene="eutC"
                     /locus_tag="ACHJWA_000057"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFO4306753.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCATTTCAAPVTSESCA
                     LDLGSAEAKVWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTLALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            65761..66420
                     /gene="eutL"
                     /locus_tag="ACHJWA_000058"
     CDS             65761..66420
                     /gene="eutL"
                     /locus_tag="ACHJWA_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFO4306754.1"
                     /translation="MPALDLIRPSVTAMRVIASVNADFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAQDTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            66433..66933
                     /gene="eutK"
                     /locus_tag="ACHJWA_000059"
     CDS             66433..66933
                     /gene="eutK"
                     /locus_tag="ACHJWA_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFO4306755.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGVTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            66979..68031
                     /gene="eutR"
                     /locus_tag="ACHJWA_000060"
     CDS             66979..68031
                     /gene="eutR"
                     /locus_tag="ACHJWA_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFO4306756.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(68037..68936)
                     /gene="hemF"
                     /locus_tag="ACHJWA_000061"
     CDS             complement(68037..68936)
                     /gene="hemF"
                     /locus_tag="ACHJWA_000061"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFO4306757.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREGGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGKDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(68940..69809)
                     /gene="amiA"
                     /locus_tag="ACHJWA_000062"
     CDS             complement(68940..69809)
                     /gene="amiA"
                     /locus_tag="ACHJWA_000062"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFO4306758.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            70023..70448
                     /gene="ypeA"
                     /locus_tag="ACHJWA_000063"
     CDS             70023..70448
                     /gene="ypeA"
                     /locus_tag="ACHJWA_000063"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFO4306759.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            70435..70884
                     /gene="yfeZ"
                     /locus_tag="ACHJWA_000064"
     CDS             70435..70884
                     /gene="yfeZ"
                     /locus_tag="ACHJWA_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFO4306760.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWRVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEVKE"
     gene            70945..71520
                     /gene="yfeY"
                     /locus_tag="ACHJWA_000065"
     CDS             70945..71520
                     /gene="yfeY"
                     /locus_tag="ACHJWA_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFO4306761.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADAVDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            71616..72515
                     /gene="yfeX"
                     /locus_tag="ACHJWA_000066"
     CDS             71616..72515
                     /gene="yfeX"
                     /locus_tag="ACHJWA_000066"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFO4306762.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNIWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(72573..73877)
                     /gene="pbp4b"
                     /locus_tag="ACHJWA_000067"
     CDS             complement(72573..73877)
                     /gene="pbp4b"
                     /locus_tag="ACHJWA_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFO4306763.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMAQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDLIAKYISGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     THWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(73882..75306)
                     /gene="murP"
                     /locus_tag="ACHJWA_000068"
     CDS             complement(73882..75306)
                     /gene="murP"
                     /locus_tag="ACHJWA_000068"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFO4306764.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PDIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPLGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(75310..76206)
                     /gene="murQ"
                     /locus_tag="ACHJWA_000069"
     CDS             complement(75310..76206)
                     /gene="murQ"
                     /locus_tag="ACHJWA_000069"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFO4306765.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNIGLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPVVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            76370..77227
                     /gene="murR"
                     /locus_tag="ACHJWA_000070"
     CDS             76370..77227
                     /gene="murR"
                     /locus_tag="ACHJWA_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFO4306766.1"
                     /translation="MLYLTKIRNAESEFTENEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATAQHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGCDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            77356..78147
                     /gene="ucpA"
                     /locus_tag="ACHJWA_000071"
     CDS             77356..78147
                     /gene="ucpA"
                     /locus_tag="ACHJWA_000071"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFO4306767.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            78305..79321
                     /gene="cysP"
                     /locus_tag="ACHJWA_000072"
     CDS             78305..79321
                     /gene="cysP"
                     /locus_tag="ACHJWA_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFO4306768.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            79321..80154
                     /gene="cysT"
                     /locus_tag="ACHJWA_000073"
     CDS             79321..80154
                     /gene="cysT"
                     /locus_tag="ACHJWA_000073"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFO4306769.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            80154..81029
                     /gene="cysW"
                     /locus_tag="ACHJWA_000074"
     CDS             80154..81029
                     /gene="cysW"
                     /locus_tag="ACHJWA_000074"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFO4306770.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEENHEH"
     gene            81019..82116
                     /gene="cysA"
                     /locus_tag="ACHJWA_000075"
     CDS             81019..82116
                     /gene="cysA"
                     /locus_tag="ACHJWA_000075"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFO4306771.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIEPRDEELALAQSA"
     gene            82250..83161
                     /gene="cysM"
                     /locus_tag="ACHJWA_000076"
     CDS             82250..83161
                     /gene="cysM"
                     /locus_tag="ACHJWA_000076"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFO4306772.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPNNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMADRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITYFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(83164..83531)
                     /gene="yfeK"
                     /locus_tag="ACHJWA_000077"
                     /pseudo
     CDS             complement(83164..83531)
                     /gene="yfeK"
                     /locus_tag="ACHJWA_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
     gene            83636..84487
                     /gene="pdxK"
                     /locus_tag="ACHJWA_000078"
                     /gene_synonym="thiJ"
     CDS             83636..84487
                     /gene="pdxK"
                     /locus_tag="ACHJWA_000078"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFO4306773.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMLVVVVTADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            complement(84530..85039)
                     /gene="crr"
                     /locus_tag="ACHJWA_000079"
     CDS             complement(84530..85039)
                     /gene="crr"
                     /locus_tag="ACHJWA_000079"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFO4306774.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(85080..86807)
                     /gene="ptsI"
                     /locus_tag="ACHJWA_000080"
     CDS             complement(85080..86807)
                     /gene="ptsI"
                     /locus_tag="ACHJWA_000080"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFO4306775.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            complement(86852..87109)
                     /gene="ptsH"
                     /locus_tag="ACHJWA_000081"
     CDS             complement(86852..87109)
                     /gene="ptsH"
                     /locus_tag="ACHJWA_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFO4306776.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            complement(87493..88464)
                     /gene="cysK"
                     /locus_tag="ACHJWA_000082"
     CDS             complement(87493..88464)
                     /gene="cysK"
                     /locus_tag="ACHJWA_000082"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFO4306777.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            complement(88649..89410)
                     /gene="cysZ"
                     /locus_tag="ACHJWA_000083"
     CDS             complement(88649..89410)
                     /gene="cysZ"
                     /locus_tag="ACHJWA_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFO4306778.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            89640..90638
                     /gene="zipA"
                     /locus_tag="ACHJWA_000084"
     CDS             89640..90638
                     /gene="zipA"
                     /locus_tag="ACHJWA_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFO4306779.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSA
                     PQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLN
                     SIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQV
                     PSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            90709..92724
                     /gene="ligA"
                     /locus_tag="ACHJWA_000085"
     CDS             90709..92724
                     /gene="ligA"
                     /locus_tag="ACHJWA_000085"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFO4306780.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KSNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTYCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            92726..92944
                     /gene="ypeB"
                     /locus_tag="ACHJWA_000086"
     CDS             92726..92944
                     /gene="ypeB"
                     /locus_tag="ACHJWA_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFO4306781.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(92941..93939)
                     /gene="yfeH"
                     /locus_tag="ACHJWA_000087"
     CDS             complement(92941..93939)
                     /gene="yfeH"
                     /locus_tag="ACHJWA_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFO4306782.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            94029..94955
                     /gene="yfeR"
                     /locus_tag="ACHJWA_000088"
     CDS             94029..94955
                     /gene="yfeR"
                     /locus_tag="ACHJWA_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4306783.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     VILSEPFFLLCHRDSALAAEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNTLMAGREGDPLYQI"
     gene            complement(94946..95287)
                     /locus_tag="ACHJWA_000089"
     CDS             complement(94946..95287)
                     /locus_tag="ACHJWA_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFO4306784.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQHI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQRQQAEKAQEKEQRLSLNVSLLNP
                     VENTTHIGIYI"
     gene            complement(95407..95482)
                     /locus_tag="ACHJWA_000090"
     tRNA            complement(95407..95482)
                     /locus_tag="ACHJWA_000090"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(95447..95449),aa:Lys,seq:ttt)
     gene            complement(95487..95562)
                     /locus_tag="ACHJWA_000091"
     tRNA            complement(95487..95562)
                     /locus_tag="ACHJWA_000091"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(95527..95529),aa:Val,seq:tac)
     gene            complement(95609..95684)
                     /locus_tag="ACHJWA_000092"
     tRNA            complement(95609..95684)
                     /locus_tag="ACHJWA_000092"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(95649..95651),aa:Val,seq:tac)
     gene            complement(95729..95804)
                     /locus_tag="ACHJWA_000093"
     tRNA            complement(95729..95804)
                     /locus_tag="ACHJWA_000093"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(95769..95771),aa:Val,seq:tac)
     gene            96063..97478
                     /gene="gltX"
                     /locus_tag="ACHJWA_000094"
     CDS             96063..97478
                     /gene="gltX"
                     /locus_tag="ACHJWA_000094"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFO4306785.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYFQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALAFIAERENQQ"
     gene            complement(97596..98021)
                     /gene="tnpA"
                     /locus_tag="ACHJWA_000095"
                     /pseudo
     CDS             complement(97596..98021)
                     /gene="tnpA"
                     /locus_tag="ACHJWA_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            98029..99237
                     /locus_tag="ACHJWA_000096"
     CDS             98029..99237
                     /locus_tag="ACHJWA_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFO4306786.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQQSLKELERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYLNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKKLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCTANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQ
                     EPTEMIQATA"
     gene            complement(99277..99669)
                     /gene="yfeD"
                     /locus_tag="ACHJWA_000097"
     CDS             complement(99277..99669)
                     /gene="yfeD"
                     /locus_tag="ACHJWA_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO4306787.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLLEAEEPLAEYAPGIRTPAYRQIISAIDNMTH
                     SEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(99671..100030)
                     /gene="yfeC"
                     /locus_tag="ACHJWA_000098"
     CDS             complement(99671..100030)
                     /gene="yfeC"
                     /locus_tag="ACHJWA_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO4306788.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            100251..100326
                     /locus_tag="ACHJWA_000099"
     tRNA            100251..100326
                     /locus_tag="ACHJWA_000099"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:100284..100286,aa:Ala,seq:ggc)
     gene            100366..100441
                     /locus_tag="ACHJWA_000100"
     tRNA            100366..100441
                     /locus_tag="ACHJWA_000100"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:100399..100401,aa:Ala,seq:ggc)
     gene            100649..102838
                     /gene="pdeA"
                     /locus_tag="ACHJWA_000101"
     CDS             100649..102838
                     /gene="pdeA"
                     /locus_tag="ACHJWA_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFO4306789.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSIYRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEVLARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(102888..104090)
                     /gene="nupC"
                     /locus_tag="ACHJWA_000102"
     CDS             complement(102888..104090)
                     /gene="nupC"
                     /locus_tag="ACHJWA_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFO4306790.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ACEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WIGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            104426..105664
                     /gene="mntH"
                     /locus_tag="ACHJWA_000103"
     CDS             104426..105664
                     /gene="mntH"
                     /locus_tag="ACHJWA_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFO4306791.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLSKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            complement(105804..106130)
                     /gene="ypeC"
                     /locus_tag="ACHJWA_000104"
     CDS             complement(105804..106130)
                     /gene="ypeC"
                     /locus_tag="ACHJWA_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFO4306792.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(106245..107501)
                     /gene="yfeO"
                     /locus_tag="ACHJWA_000105"
     CDS             complement(106245..107501)
                     /gene="yfeO"
                     /locus_tag="ACHJWA_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFO4306793.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            107705..108670
                     /gene="glk"
                     /locus_tag="ACHJWA_000106"
     CDS             107705..108670
                     /gene="glk"
                     /locus_tag="ACHJWA_000106"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFO4306794.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            108889..109215
                     /gene="fryB"
                     /locus_tag="ACHJWA_000107"
     CDS             108889..109215
                     /gene="fryB"
                     /locus_tag="ACHJWA_000107"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFO4306795.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            109237..110484
                     /gene="fryC"
                     /locus_tag="ACHJWA_000108"
     CDS             109237..110484
                     /gene="fryC"
                     /locus_tag="ACHJWA_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFO4306796.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            110499..111584
                     /gene="ypdF"
                     /locus_tag="ACHJWA_000109"
     CDS             110499..111584
                     /gene="ypdF"
                     /locus_tag="ACHJWA_000109"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO4306797.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVLISR
                     ESAHILVDSRYYADVEARTQGYQLHLLDATHTLTTIARQIIADEQLQTLGFEGQQVSW
                     ETAHRWQSELNAKLVSATPDVLRQIKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKTSFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISAIRPGVRCQQVDEAARRV
                     ITEAGFSHYFGHNTAHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            111584..112621
                     /gene="ypdE"
                     /locus_tag="ACHJWA_000110"
     CDS             111584..112621
                     /gene="ypdE"
                     /locus_tag="ACHJWA_000110"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO4306798.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHSAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFNYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            112646..115141
                     /gene="ptsP"
                     /locus_tag="ACHJWA_000111"
                     /pseudo
     CDS             112646..115141
                     /gene="ptsP"
                     /locus_tag="ACHJWA_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311290.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            complement(115144..116001)
                     /gene="ypdC"
                     /locus_tag="ACHJWA_000112"
     CDS             complement(115144..116001)
                     /gene="ypdC"
                     /locus_tag="ACHJWA_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4306799.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRLVEGEMLFIPARAANLPVNNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(116014..116748)
                     /gene="ypdB"
                     /locus_tag="ACHJWA_000113"
     CDS             complement(116014..116748)
                     /gene="ypdB"
                     /locus_tag="ACHJWA_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFO4306800.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            complement(116763..118460)
                     /gene="ypdA"
                     /locus_tag="ACHJWA_000114"
     CDS             complement(116763..118460)
                     /gene="ypdA"
                     /locus_tag="ACHJWA_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFO4306801.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            118837..120075
                     /gene="alaC"
                     /locus_tag="ACHJWA_000115"
     CDS             118837..120075
                     /gene="alaC"
                     /locus_tag="ACHJWA_000115"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFO4306802.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAILESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            complement(120140..120211)
                     /gene="ypdK"
                     /locus_tag="ACHJWA_000116"
     CDS             complement(120140..120211)
                     /gene="ypdK"
                     /locus_tag="ACHJWA_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFO4306803.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(120567..121487)
                     /gene="lpxP"
                     /locus_tag="ACHJWA_000117"
     CDS             complement(120567..121487)
                     /gene="lpxP"
                     /locus_tag="ACHJWA_000117"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFO4306804.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            121840..122082
                     /gene="yfdY"
                     /locus_tag="ACHJWA_000118"
     CDS             121840..122082
                     /gene="yfdY"
                     /locus_tag="ACHJWA_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFO4306805.1"
                     /translation="MFYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            complement(122159..122386)
                     /locus_tag="ACHJWA_000119"
     CDS             complement(122159..122386)
                     /locus_tag="ACHJWA_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020232163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4306806.1"
                     /translation="MACNVFAFSNSGGGSETSYKEPEKTSAMTTTHSTKFQPSQAILF
                     KMREDAPPLNLTEETTPPFPTKANYLIHPVR"
BASE COUNT        29323 a        31249 c        33009 g        28896 t
ORIGIN      
        1 aacatgataa acgtaataat tcattaatca gggttatttt gtttgatata tatcaattgg
       61 aactttcata tgacacttag aatcaatgct ctctatagtg atgaataatc atcattcgaa
      121 gtcaggtggg atgcctgtct gaatacacct ccttcaggat gtgggggatt ctgctgagca
      181 tctatgaaac tgaatgcaac ttatataaaa atacgtgata aatggtgggg gcttccgctg
      241 ttcctgcctt ctttaatctt gcccattttc gcccacatta atactttcgc gcatatttct
      301 tccggtgagg tttttctctt ttatctgcct ctggcactga tgatcagcat gatgatgttt
      361 ttcagctggg cggcattgcc agggatcgcc ttagggattt ttgtccgcaa atatgcagag
      421 ctgggttttt acgaaacgct ctcattaacg gctaatttta ttatcattat cattctctgt
      481 tggggcggtt acagggtctt tactccccgg cgtaacaacg tttcacatgg tgatacccgt
      541 ttaatttccc agcgtatatt ctggcagatt gtgtttcctg caacgctgtt tctgatactt
      601 ttccagtttg ctgcatttgt aggattactg gcgagcagag aaaatctggt tggcgtcatg
      661 ccttttaatc tcgggacctt aatcaattat caggccttac tggtgggcaa tctgattggt
      721 gtcccgctgt gctacttcat cattcgggta gtgcgaaatc cgttttattt acgtagttat
      781 tattcgcaat taaaacagca ggttgatgtc aaagtcacca aaaaagagtt cgcactctgg
      841 ctgctggcat taggtgcttt actgttgctg ttatgcatgc cgttaaatga aaaaagcaca
      901 atttttagca ccaattatac cttgtcatta ttgctgcccc tgatgatgtg gggagcgatg
      961 cgctatggtt ataagctgat ttcgctgctc tgggcggtcg tgttgatgat cagcatccac
     1021 agctatcaaa attacattcc catttatcct ggctatacca cgcagctgac cataacctcc
     1081 tccagttatc tggtattctc ttttattgtc aattatatgg ctgtactggc aacccgtcag
     1141 agaacggtag tcagacgcat tcagcgcctt gcgtatgtgg acccggtggt tcatctgcca
     1201 aatgttcgcg ccctgaatcg cgcgttgcgt gatgccccct ggtctgcgct ttgttattta
     1261 cgcatccctg gcatggaaat gctggttaaa aactatggca tcatgctgcg gattcaatac
     1321 aagcaaaaac tttctcactg gctgtcaccc ttgctggaac caggtgaaga tgtttatcag
     1381 ctttcgggta acgatctcgc gctgcgactg aatacagaat cgcaccagga gcgcattacc
     1441 gcactggata gccatctcaa gcaatttcgt ttcttttggg atggcatgcc gatgcaaccg
     1501 cagattggcg tcagttactg ctatgtgcgc tcgccagtga atcatatcta cctgctgctg
     1561 ggagagctaa atacggtcgc cgaactttcc atcgtgacca acgccccgga aaatatgcag
     1621 cgtcgcgggg caatgtattt gcaacgcgaa ttgaaagata aagtcgcgat gatgaatcga
     1681 ctacagcagg cgctggaaca caaccatttt ttcctgatgg cccagccgat taccggtatg
     1741 cgtggtgatg tttaccatga aattcttctg cgcatgaaag gtgagaatga tgaactaatc
     1801 ggccccgata gcttcttgcc ggtcgcgcac gaatttggtt tatcgtcgag tatcgacatg
     1861 tgggtcattg agcatacgct gcaatttatg gctgaaaaca gagcgaagat gcccgctcac
     1921 cgttttgcta ttaatctgtc tccaacctcg gtatgtcagg ctcgttttcc tgttgaagtc
     1981 agtcagttgc tggataaata tcagattgaa gcgtggcaac ttatttttga agtcaccgaa
     2041 agtaatgctc tgaccaatgt taagcaggcg caaatcacct tgcagcatct tcaggaatta
     2101 ggctgccaga ttgcgattga tgatttcggc accggctacg ccagctatgc gcggcttaaa
     2161 aatgtgaatg ccgatctgct taaaattgac ggcagtttta tccgcaatat tgtgtcaaat
     2221 agtctggatt atcagatagt ggcatcgatt tgccacctgg cgcgaatgaa gaaaatgcgg
     2281 gtagtggcag agtacgttga aaacgaagag atccgcgagg cggtgctctc tttggggatc
     2341 gattatatgc agggttatct tattggtaag ccgcaaccgt taattgatac gctgaatgaa
     2401 atcgaaccca ttcgcgaaag tgcctgaata atgcgggccg acatttctcg tcggcccgca
     2461 aagcattaag ctgcgatttc aggtgtactt tcttcttcaa ttttcaaccg ccagccagcc
     2521 acgccttccc agtattcttg ctccttttcc agatcaagca gtaccagcgc attctgacta
     2581 aaccagtcat gcgggaaacg cagtgtccag tgactgtcat cagtaatcag cgtcaatgtt
     2641 ggcggtgtgg tggttgcctg acgctgattg ttgagtaata cgccaaggcg caatagctga
     2701 atcagtggca ggaactgttt cttcttaaac aaggtaaagc gcggcagatc gtcgagttta
     2761 atcgctttac ggtgatagcg caccagtgtc gccatcatca gctgctgttc ctgattaaaa
     2821 cccggcaagt cgctgttttg tagaatataa gcggagtgac gatgcaaacc gctgtggttg
     2881 atattcaacc cgacctcatg cagcatggcg gcccatcgca gtagcgcctc cagttgcgga
     2941 tgcgccagct tcggttgctg ttcccgccac tgttcgtaca tttgcatagt ggtatccagc
     3001 acccgtcggg cctgttcgct gtcgatgtga tactggttgg cgaggctgct ggcggtgcga
     3061 ctacgcacat cctgatgacg gaaacgtcct tccatttcat acagtacgcc ttcgcgtaac
     3121 gccccgtcag aaaggcgcag ttcacggatg gctaaagcat caaacacacc gcataaaatc
     3181 gctagtccag gaacgaagac tgttttccgc tcttcggaaa gacctggtaa actcagcgat
     3241 gcgaaattac ggtgccgtaa aacttctttt accagttttt ccagacgttc cggggtaatt
     3301 atcccgtctt tctcgcccat ttccattaac acttcatggg cggcttttat ggtaccggaa
     3361 gcgcccattg ccacgttcca gccctgaata cgaaattgcc aggttaaagt ttccagtttt
     3421 tgtgctgccg ccatgcgagc gcgctgaaaa ttctctttat tgatgacccc gccagggaaa
     3481 taaagctggg caaagctgac acaccccatc cggcggcttt caacgagaat cggttcgaaa
     3541 ttttcaccaa tcaccagttc tgtagaaccg ccgccaatat caataaccag tttgcgacct
     3601 ttttccggtt gggtatgttc cacgcccata aaaatcagac gggcttcttc attaccggaa
     3661 ataatttcaa tcgggtaggg aatgaccttt tccgcacgtt tcagaaagtc agtggcgttc
     3721 agcgcctgac gcagcgtatg ggtaccaact atacagacgc tggcaggaga aaacccttgt
     3781 agccgttcgg caaacagcga cagacagttc aaaccgcgcg tcattgcctc ttcactcaac
     3841 atattatctg gccccaggcc gtccgccaga tgcacccgct gtttcaggcg gccaataatc
     3901 tgcatggcac catctaccac acgggctatg accatgtgaa aactgtttga accaagatcg
     3961 accgcagcaa actcctgcgg acgaggggat ttatcgtgta ttggcatagg gttattcagg
     4021 ttgttcgagt gatttgatgt agtcataaat cgccaactgc gcccgtactt tgcggcgatt
     4081 gccgcgggga acgtagcgat tactgagttc tttatcgata taacgtgctt tgaccgtatc
     4141 gctgaacaat atgtcgatga tgtccagcac ccgctgcttc aggcgcggat cgagcagcgg
     4201 cgtcgccact tcaatacgat aatcaatatt gcgcgtcatc cagtcggcgg aagaaaggta
     4261 gaccttttta tcgccgccat tttcaaaaat ataaacccgg tcatgttcaa ggtaacggtc
     4321 aacaatactg atggcacgaa tgttgtcgct aatgccttcc agattgggga tcagcgaaca
     4381 cattccgcga accagcagat taaccggtac gccggagctg gaggccgcat acagacgatc
     4441 aaccaggcct ttatcgacaa ggttatttag cttcagggtg ataccactgg gcagcccttg
     4501 ctgcgcgttg gcgatctcgc ggtccaccat ttcatacaac aggcggcggg agttttgcgg
     4561 cgacaccatt aaataatcaa atgtcaccgg acggtatggg ttttcaataa agttaaatac
     4621 ccgccgtact tcgttggtga tgcgcgcatc ggcggtcagc aacgaatagt cagtataaag
     4681 acgcgcggtt ttttcgttaa agttcccggt cccgatgtgt gcataacgca ccacttcacc
     4741 gttttcttta cgtgaaatca gaaacagttt ggcgtgaatt ttcagccccg gcgcagagaa
     4801 gataacgtgc acgcctgctt cggtcaggcg cttcgcccag tgaatgttgg cttcttcgtc
     4861 aaaacgtgcc tgtagctcaa ccaccacggt gactttctta ccgttgtgtg cggcgtggat
     4921 catcgagtcg atgatgcgtg aatcttttgc cacacggtaa atgttgattt taatcgccag
     4981 cacgctcgga tcgaacgagg cctgacgcag cagttccagc acatgctcaa aggtgtgata
     5041 aggataatag agcaacacat cgcgttcgcg aatggcatca aaaccattgc ggaactgggc
     5101 tttatcaaac caaatatggc gtaaacgcgg cagtggtttg ttcaccagat tggctttgcc
     5161 gacattgggg aaattaataa agtctttaaa attatgataa cgaccgccgg ggacgatgga
     5221 gtcgtagcgg gaaatagtca gtttttcgcg taacacttca accagcgcat tgggcatatc
     5281 gcgctgataa acaaaacgca ccggctcagc agttaaacgc tgcttgagac tggaagacat
     5341 caactccatc aggctggctt ccatctcatg cactaaatcg tattcggcat cgcgggtcat
     5401 cttcattgaa taggcattca gcgcgtcata atcaaagaag cctttgaaaa tatcatcaag
     5461 gcagtaacgc agaatgttat ccagaagaat cattggcttg cgtcgacgcg gcgcttctgg
     5521 cggtaaattc acaaagcgcg gcactttatc tgatgggatc tccagaagcg cgtaacggat
     5581 ggtatcgcca cggataattt ccaccgccag ataggtgtaa tcatctttca ggaactgcac
     5641 taagtcagtg tcaggattga ttaaaatcgg cgtaatgtgc tgacgcagat actgcttaaa
     5701 ataatgacgc agccagtttt gttgattgac ggagagctgg cgttcattaa tcaggaagat
     5761 ctggttgcgc gccatctcca gcaatagctc gttgtagagg ccgtcgaatt cctgatcggc
     5821 tttcagcacc cgggactgaa ttttgcccag taaatggcgg gaatgagagt tggagccttg
     5881 ttcttcgcta ataatgatgc gtcgcttcag ttcagcgaag cggactttat agaactcatc
     5941 aaggttattg gaatagatcc ccaggaaacg catcctttca atcagcgggt tagatttgtc
     6001 cgccgcttcc tgaagcacgc gttcattgaa cgataaccaa ctgagctctt tttcgatgta
     6061 tagcttttcc tgacccatta ccacttttac tccgttttat tcacgggaca ctgcctggat
     6121 attatggcga gcaatattgg cagatgtcca actatgccag aaaaggggga aaaaaactgc
     6181 tggagcttgc gatttacagg tgaattgaaa aacgccagcg gcagagctgg cgctttaact
     6241 acggggcatt actcgtcggc agcgtagccc tgcggcggca gacgttgacc atccagccac
     6301 gcggcgtttt cgtgcatttt cagacgacca tcggcaaacc agctaatcac cagtggataa
     6361 atggcgtgtt cctgggtttg cacgcgggcg gtgacgtcat cctccgtatc accagcaaat
     6421 accgggactt tcgcctgtaa aataaccggg ccaccgtcca gttcatcggt gacgaaatgc
     6481 accgatgtac cgtgctcttc atcgccattt tccagcgcct gacgatgggg atgtaatccg
     6541 ggatatttcg gcagcagaga agggtgaatg ttcagcaaac gcccggcata gtgggagaca
     6601 aatgccgggc tgagaatgcg cataaaacca gccagcacga ccacatcggg tgcgtacatg
     6661 tcgatttcat gaatcaactc ccggtcatag gcttcacgac tgtcaaacgc gctggcgatg
     6721 agcgtatgcg ttgcaatacc cgcctggcgg gcgcgttcaa ggccgaacgc gtcggcctta
     6781 ttgctgaaaa ctgcccgtac ggtgccttta attttgttgg ttttacaggc gtcaataatt
     6841 gcctgcaaat tacttccgtt gccggaaata agcaccacaa tattcattat tcgataacca
     6901 cgcgttgttc ggaatcagag gctttgatga taccgatttt ccacgcgttt tcaccgttgg
     6961 cattgagcag ggcgagggct ttgtccactt ccggagcagg cagggcaata atcatcccga
     7021 cgccgcagtt gaaggtgcga tacatttcat ggcgctcaac gttaccagcc gtttgcagcc
     7081 agttgaacac ttccggccac tgccaggagg attcatcaat cactgcctgg gtattatctg
     7141 gcaatacgcg cggaatgttt tcccaaaagc cgccgccggt caggtgcgca atggcattaa
     7201 catcgacctt ttcaatcaac tccagcactg acttcacgta aatgcgggtc ggtgccagca
     7261 gatgatcggc taatggctta ccatcaagtt cggtggtttg cggatcacaa ccgctgactt
     7321 caagaatttt gcgcaccagc gaatagccgt tcgagtgcgg accgctggaa ccgagtgcaa
     7381 tcagcacatc gccgtcgctg actttagagc cgtcgatgat ttctgatttt tctaccacgc
     7441 ccacgcagaa acccgcgaca tcgtaatcct caccgtgata catccccggc atttctgccg
     7501 tttcgccacc caccagtgaa cagcccgatt gcagacaacc ttccgcaatg ccgctgatca
     7561 ccgctgaagc ggtatcaaca tccagttttc cggttgcgta atagtcgagg aaaaacagcg
     7621 gttctgcacc ttgcaccacc aggtcattaa cgcacatggc gaccagatca ataccaatgg
     7681 tgtcgtgacg ttttaagtcc attgccagac gcagcttggt acctacgccg tcagtgccgg
     7741 aaaccagcac gggttcacga tatttttgcg gcaatgcaca cagcgcaccg aagccgccca
     7801 gaccgcccat cacttccgga cgacgcgttt tcttcactac gcctttgatt cttccaacca
     7861 gagcattacc cgcgtcaata tcaacaccgg catctttgta gctaagagag gttttatcgg
     7921 tcactgcttg ggtccccacg cgttacttgc ggtagaaaaa taaaattcgg cgcaattcta
     7981 acagggaaag caaacgtttg cgagactgct ttacacaacc tttttgcacg tcttttcccc
     8041 aggcgcgcgg cgaaagaaga cttgtgccag ggcaaaggtt agttttcgga tggaataatc
     8101 ttctttcata accatttgaa tataaaataa ctttatctca aaccgttatc attttgacta
     8161 aagtcaacga aaataatatt gccgccttga agaaaggagg tataatccgt cgattttttt
     8221 tgtggctgcc cctcaaagga gaaagagtat gaagatcgtg gaagtcaaac acccactcgt
     8281 caaacacaag ctgggactga tgcgtgagca agatatcagc accaagcgct ttcgcgaact
     8341 cgcttccgaa gtgggtagcc tgctgactta cgaagcgacc gccgacctcg aaacggaaaa
     8401 agtaactatc gaaggctgga acggcccggt agaaatcgac cagatcaaag gtaagaaaat
     8461 taccgttgtg ccaattctgc gtgcgggtct ggggatgatg gacggtgtgc tggaaaacgt
     8521 tccgagcgcg cgcatcagcg ttgtcggtat gtaccgtaat gaagaaacgc tggagccggt
     8581 accgtacttc cagaaactgg tttctaacat cgatgagcgt atggcgctga tcgttgaccc
     8641 aatgctggca accggtggtt ccgttattgc gaccatcgac ctgctgaaaa aagcgggctg
     8701 cagcagcatc aaagttctgg tgctggtagc tgcgccagaa ggtatcgctg cgctggaaaa
     8761 agcgcacccg gacgtcgaac tgtataccgc atcgattgat cagggactga acgagcacgg
     8821 atacattatt ccgggcctcg gcgatgccgg tgacaaaatc tttggtacga aataaagaat
     8881 aaaaataatt aaagccgact ttaagagtcg gctttttttt gagtaaagcg cctataacac
     8941 ataatacaga ggataatact atgacgcgcc gtgctatcgg ggtgagtgaa agaccgccac
     9001 ttttacagac aatcccgctt agtttgcaac atttgttcgc catgtttggt gcaaccgtcc
     9061 tggtgcccgt cttatttcat attaacccgg cgactgtact gttatttaac ggtattggaa
     9121 cgctgctgta tctcttcatc tgtaaaggga aaattccggc ttatcttggt tccagctttg
     9181 cctttatttc accggtattg ttactgttgc cgttagggta tgaagtcgcg ctgggcggct
     9241 ttattatgtg cggcgtgctg ttctgcctgg tttcttttat cgtgaagaaa gcggggaccg
     9301 gctggctgga cgtgctgttt ccacctgcgg caatgggcgc aatcgttgcc gtcatcggtc
     9361 tggagctggc gggcgtagct gccggtatgg cgggtttact cccggctgaa gggcaaacgc
     9421 cagactccaa aaccatcatc atctctatta ccaccctggc ggtcacggtt ttaggttccg
     9481 tgctgttccg tggtttcctg gcaattatcc cgattttaat tggcgtgctg gtggggtacg
     9541 cgctctcttt cgcaatggga attgtcgata ccacgccgat tattaatgct cactggtttg
     9601 cgctgccaac cctctatacg ccgcgcttcg agtggtttgc cattctgact attctgccag
     9661 cggcgttagt ggttattgcc gaacacgtag ggcacctggt agtaacggct aatatcgtca
     9721 aaaaagatct gctgcgcgat ccaggtctgc accgttcgat gtttgctaac ggcttgtcga
     9781 ccgtgatctc cggcttcttt ggctccacgc caaacaccac ctacggggaa aacattggcg
     9841 tgatggcgat cacccgtgtt tacagtacct gggttatcgg cggggcggcg attttcgcta
     9901 tcctgctttc ctgcgtcggt aaactggctg ccgctatcca gatgatccca ttgccggtga
     9961 tgggcggcgt ttcgctgctg ctttatggtg tcatcggtgc ttccggtatt cgtgttttga
    10021 tcgaatcgaa agtggattac aacaaagcac agaacctgat cctgacttcc gtgattttga
    10081 tcatcggcgt cagtggcgcg aaggtaaaca ttggcgcggc ggagctgaaa ggtatggcgc
    10141 tggcgaccat cgtcggtatc ggcttaagtt tgattttcaa actgattagt gtgctgcgcc
    10201 ctgaagaagt ggtgctggac gcagaagacg cggatataac agacaaataa tgtaccgacc
    10261 gggcagtgtt gctgcccggt tcaaacatca tgggattctg tggtaaactt ctcgcgattt
    10321 tgtgaaatcc tggttgaggt atctctgaac acaccggcac agctctcttt gccactttat
    10381 cttcctgacg acgaaacctt tgcaagtttc tggccggggg ataactcctc tttactggcc
    10441 gcgctgcaaa acgtgctgcg tcaggaacat agcggttaca tctatctctg ggcacgcgaa
    10501 ggcgcggggc gcagccatct gctgcacgcg gcttgcgcgg aattgtcgca gcgtggcgat
    10561 gcggtgggct atgtcccgct ggataaacgc acctggtttg ttccggaagt gctcgacggt
    10621 atggagcatt tgtcgctggt ctgtatcgac aacattgagt gtattgcagg cgatgagttg
    10681 tgggagatgg cgattttcga tctctacaat cgaattctgg aatcgggcaa aacacggttg
    10741 ttgatcaccg gcgatcgtcc accgcggcag ttgaatctgg gattaccgga tctcgcgtcg
    10801 cgactcgact gggggcagat ctacaaattg cagccacttt ctgatgaaga taagttgcag
    10861 gcgctacagt tacgcgcgcg tttgcgtggt tttgaactgc cggaagatgt ggggcgtttc
    10921 ttgctgaagc ggctcgacag agaaatgcgc acgctattta tgacgttgga tcagttggat
    10981 cgtgcgtcga ttaccgcgca acgtaagctg accattccgt ttgtgaaaga aattctgaag
    11041 ttgtagataa ggtgtttatt gtcggatgcg gcgcgaacgc cttatccgaa ctacgcgaag
    11101 ttaatccata gaattcgtag gccggataag acgtggcaag cgtcgcatcc ggcaatgtgc
    11161 ttcaactagc gatggagcac atcttaaccc acgatttcca gtacctgctc cggtggacga
    11221 ccaatccgcg ctttgccatt cgcgaccaca atcgggcgct ccatcagctt cggattatcc
    11281 accattgcct gaatcagcgc ctcttcgcta agcgaactgt cagccaggtt cagttcttta
    11341 taaagatcct ctttctggcg catcaattca cgggcgctat tcatccccag cattttcagc
    11401 aaatcgcgca gcgttgccgc atcggcgggt gtctcaaggt agagcaccac ttctggctcc
    11461 acgccgtttt ctttcagcag gttcagcgtt tcccggctct ttgaacagcg tgggttatgg
    11521 tagattttta cctgtttggt cattgttctt cctttaatgc gaattacatc ttggtataag
    11581 gcttaaagcg ttcctgcagc tggcgcaact ggtcgatgcg cgcatcgtaa cgcgcttgtt
    11641 gcaggctgcc taatttcacc tgcgaactgg cgctactcaa cagcgaaatg gcctgatcga
    11701 gtcgtccggc gagcgcataa ccttctgctc gcgcagccag ctcctgatcg cggttattta
    11761 gcgcggcttc cgcctgtgcc agcaaatccc agccgttgct gtcatcttta ttattaaagg
    11821 tgtagcgatt aagaatattg gccgcttctt gtggttgacc gccttgtaga taagcgttcg
    11881 ccaggttgag ctgcaacaca ggattggtgc gcaaatcgcg ggcatttttc agacgattga
    11941 tcgcctcatt ggctttgttt tgcccaagat cgatatcagt agccagatcg agataccatg
    12001 cgttgccagg ttctgccgcc agtaacggtt gcagcgtttt tcgcgcctcg tcgtatttat
    12061 tggcttccat cgcctgtaaa gcacgaccat attgcgccgc tcgttgctga cgaacgtttc
    12121 ctttcgccca ttcatccagc aaatcactgg tgagctggtt acgtccggaa ttatacatcc
    12181 ccagtgtgcg cgctttcgcc agatagaaat cttccgacga ctgcaccacc atcgggcgca
    12241 tctgattagc acggttgcgg gcatctgcca gacgactttc cggcaacggg tgagtcagta
    12301 aaatttccgg cgggcgcgag gagtaacgcg cctgatcgag taatttttcg aggaaggttg
    12361 gcatcgcctg cggatcgaat cccgagcgtt gcagcacctg aataccaatg cggtccgctt
    12421 cctgttcatt ttgctgggtg aaactgatca tcccctgacg cgttcccgcc agtgtaccgg
    12481 tcagcgccgc catccccgcc tgcggactgg ccatcgccag taaaatagaa cctaacgcgc
    12541 cgacccaggt cagcggcgcg ttgcgctgct gatcttccat cgctcgcgcc aggtgacgtt
    12601 gggtgacgtg ggagatttcg tgcgccataa ctgaagccag ttgactttcg ttatcggaat
    12661 aacggaacag ggcagagtgc agcaccacgt tgccgccaaa gaaagcaaag gcgttaattt
    12721 cgtcgttgtt gatcagaaag aaatgaaacg gtgtcttaac cgaattggca tgcgaaacca
    12781 gacgcatccc cagcgaatta atatattgcg ttaacagcgg gtcattaatt aacggcgcgc
    12841 tgccgcgtag ctggcggaca taatagtcgc ccatctgcat ttcctgacca atggaaagcg
    12901 tgcttcctgc ggaggttccc atatccggca aggtgtctgc gctgtcggca aatgccgggg
    12961 ctacctgacc aatggtcata gcagcaatga gggttgcaac caggtttttt ttcaactgcc
    13021 tgaacataac ctctatcctg tatttctgaa gatagtcatt tgaccgatac tgtgaaatat
    13081 cgttcccacc gcgccgcctt tgagtgtaac ggcgaagatt agcccactca aatgactttt
    13141 cactttcctg aatccgtcat aaaaagcgaa gtcttttttg tgacatttcg atacaattgc
    13201 ggatcgcaat cccgctcttg aggttcaggg aaggtcttta tgctcgaaat gttgatgcaa
    13261 tggtatcgcc gccgttttag cgacccggaa gcgattgcct tgctggttat tttagttgcc
    13321 ggatttggca ttatcttttt ctttagtggc ctgcttgctc cgttgctggt ggctattgtg
    13381 ctggcctatt tgctggaatg gccaaccgtg cgcctgcaat ctattggctg ctcccgccgc
    13441 tgggcgacgt cgattgtatt ggtggttttc gtcggtatat tgctactgat ggcgttcgtg
    13501 gtactgccta tcgcctggca gcagggcatc tacttaatcc gcgatatgcc ggggatgctc
    13561 aataagcttt ctgactttgc cgccacgttg ccgcgccgct atccggcgtt aatggatgcg
    13621 ggcattattg atgcaatggc cgaaaatatg cgcagtcgga tgctgaccat gggcgattcg
    13681 gtggtgaaaa tttccctcgc ctcgctggtc ggtttgctga ccattgccgt ctatctggtg
    13741 ctggtaccat tgatggtctt cttcctgctg aaagacaaag agcagatgct gaatgccgtt
    13801 cgccgggtgc tgccgcgtaa ccgtggactg gcaggacagg tgtggaagga gatgaatcaa
    13861 caaatcacca actatatccg cggcaaagtg ctggagatga tcgtagtagg gatcgccacc
    13921 tggctgggat tcttactctt cgggctgaac tattcgctgc tgctggcggt gctggtcggc
    13981 ttctcggtac ttattccgta cattggcgca tttgtggtga ccattccggt ggttggcgtg
    14041 gcgctattcc agtttggtgc aggcactgaa ttctggagct gtttcgcggt gtatctgatc
    14101 attcaggcgc ttgacggcaa cctgttagta ccggtgttgt tctccgaagc ggttaacctg
    14161 catccgctgg tgattatttt atcggtggtg atcttcggtg gtttgtgggg attctggggc
    14221 gtattcttcg ccattccatt ggcgacgctg atcaaagccg tgatccacgc ctggcccgat
    14281 gggcaaatag cgcaagaata atcttcagcc cgacgaactg tttcgccggg ctgatttttc
    14341 aggattcctg acgtaaataa atagcccgat aacaaatact caccagcact gcaccgccga
    14401 taatattacc cagcatcacc gggagcagat tggcggtaat aaaatggctg accgtcagtg
    14461 caggaaaatt gtctgcgcta ctgtgcgcca gttgccagaa ggaggtaggg gcgaaatggc
    14521 gaatggcaat ggcgaacgga atcacaaaca aattggcgat acagtgctca aagccactgg
    14581 cgacaaacag ggtgatgggc aaaatcatgg cgacgatttt gtcgcataac gaacgcccgc
    14641 aataactcat ccacagcgcc aggcaaacca tcagattgca cataatgccg aggctgacag
    14701 attcagtaaa tgtatgatgc attttgccct cggcgcagtg taaaaccgcg actccccaca
    14761 tcgcgttttc actcatcacc agcccggaaa accagattaa caaactgaaa caggcaatac
    14821 ctgccagatt accgcaggcc accagaagtg cgttaatcag ccaagttcgc caactaataa
    14881 cgccccgact ttttgccatc accgtcatta ccgacgaggt gaacaggctg gtgccgcaaa
    14941 ccgccagcaa aataaagccg agtgtaaagc aaaggccgcc caccagatgg gttaatgcct
    15001 gtgaagacgg ggcatcggcg ataacggaaa ggtaaaaagt aaatccgatc gccataaaca
    15061 cgccagcgga catcgccatt aaaaagaccg acactttact gcgggcaatt ttatgggcgg
    15121 caatccgttc agcgattgcc gcttttctgg cgctcaactg caagtcgaaa gagagtttgt
    15181 ttctcatgca tactcctgca aaagcagatt acaacacctc gcgaaccgag atccccagac
    15241 gctgcattcg tgacagcagc gtggtgcgct tcatccctaa tcgtgtcgcc gcgccacggg
    15301 gcccggcaac aatgccattg gtttctcgca atacctgaac aatgcgctga cgttcttctt
    15361 cgtcattctc tggcgtcgtc gggtgcagca actgcgccat tgaactgcga agcgctgaat
    15421 cttcatttag cgtcggcagt aaacggcttt gtcggacatt aagatgtaaa ttcagactgt
    15481 taccgcgagt cagcagtacc gctcgctcaa tcacgttttc cagctcgcgc acgttgcccg
    15541 gccaatccca cgacatcaac tggcgtagtg cctcggtcgg gatggcgtca attgcgcgat
    15601 tcatatggcg cgccattttt tgcgtgaaat gttttgctaa aagagggata tcttccggac
    15661 ggtctcgcag cggcggcaat tccagtggga agacattcag gcgataaaag agatcgctgc
    15721 gaaactggcg atcttcaacc atttgccaca aatcacggtt ggtggcggca atgactctga
    15781 cattcaccgg gatcgttcta ctcccgccga gacgctcaat ttcccgctcc tgcaatacgc
    15841 gcagcaattt aggctgaagt tctaacggca gatcgccaat ttcatcgaga aacaacgtac
    15901 cgccatcggc aatttcaaaa cgaccacgat gggtattaat cgcaccagta aaggcccctt
    15961 tgtcatgacc gaataactca ctttccagaa gactggcggg gatggcagcg cagttgattt
    16021 tgaccagcgg cttgtcgcgt cgcgggctaa gttgatggat cgctctggcg atcacctctt
    16081 tgccggttcc ggtttcacca caaatcaaca ccgtactgtc gctcttcgcc acaatatcta
    16141 cctgctggag caggtcttcc attgcctggc tttgatagat aatgtcaccg acgcccagat
    16201 tcgaaaggag ctgctcgcta agctggtggt tttcttgctg caaactttcc tgcaaatcgg
    16261 tcatgctacg ccaggcatcg gcattgccaa cggcaatagc gatgcgatcg gctatgtgtt
    16321 gtagtagctg gcagttttcc tcactaaaga gagtggtaga ggtatgcgcc agcaacaatg
    16381 cacccggtgt atggttgcca aaggtaagcg gtatcaggag cgaagattcg cagccgttag
    16441 atgcaagtaa gtgtaataac gggtcgcgtt gccagagaaa cagatcgtct gcacggtgcg
    16501 tcaaggtcgg ttgtcgggtt tgtagcgttt gtgtcagcaa tacactgtcg ccaggcatat
    16561 tgcgtggcag acacgcacaa tgtgaggcag aaagatcgct gcaccacagg ctgaacttct
    16621 cgttctttcg atgatcgccc agtaccatac tgactgaagc cagaccgaaa aaatgatgga
    16681 tctcacgagc gacgtcagcg atcagatcgt cgagatcaag atgtgacagc actgcattag
    16741 tgatatccac cagtacccga tgatgatcgc gatctttctc cagcaaacgc agcgccacct
    16801 gttgttcttc atcattgagc catgattgca gaaccggggt gataatgcgt gccagccagt
    16861 taaacagtgt gcaatcctgg ggagtaaaac gactgccgtg ggtgcgaata aagcgtagct
    16921 ccgccattcc actactgccg agaaggacgg cgtgacgcga ataatcgacc atctcgccct
    16981 gatactctgt tgttgccgtc acatcgctgc catcatcatg gtagcggtaa tagaagttgc
    17041 ttttgagaac atgcagttca ataagttgta ccgggctgaa cggcggcggc agcgggatga
    17101 aggcgcgtaa taaagacgcc ttgccgtgtt tctgcgctaa ccgctctgcg agcattccgt
    17161 ttaccgcttc aattgttatt ccttgcggcg gggcaaacat cgcctcgtct gacatagcca
    17221 tcaacagtcc ttattaatga gaaatccttc tcacgcaatt tttctggcca tcagatagat
    17281 agccggttcc agcacaattt catccaacag atgaatgaat tgaatgctac gcgcttgcca
    17341 cacagacgtc gcgtcatctg ccagtggcgc gaatgcccgg gtcaacaggg cggtgtggct
    17401 ggcgtcaata acaatctcac caaaagtcat cacccccagc atcttacgtc gggcttgctc
    17461 ctcttcgacc agtgcaagcc aatcttcgta ttccgtcagt gggcagcgga aggtgacatt
    17521 cagacaatca atcacgccaa cgtgatgacc gatagccagc gagtaataca ttacctggga
    17581 gctgtgttcc ggcatctcgt cgctactggc gacaaacttt tttcgcagcg tccagaaaac
    17641 aatttccccg cactcttcag tcatgtaacc cccggagtac gcgaaagagt tgctgaacga
    17701 tactattgag acgtggatcg tcggcatcgc gcaaccaggt gttaacccga tttcctgtag
    17761 gatcgttgct taaatggcgc aggagccgat cgcaaatttc ttgcccctga cgatagcccg
    17821 ccagccgccg cgcttctcgc tcaatggcga tacgctgtga tggcgggatc tgcggccaca
    17881 acggttgcat agtcaccccg gtgggatcgc gataatccac cgcgtgaatc ttctgttgca
    17941 gcaaaccgag cgccacggcg aaaccgtgaa tggtggcggc cggtgttggc gggcagccgg
    18001 ggatccaaac atcaatgggg acaatggtgt cgctgccgcc ccagacgctg tagagatcgt
    18061 ggaaaatacc gccgccgaca ccgcacgcgc cgtaggaaac acagatttta tgatcggggg
    18121 cagactcata cgcccgaagt gcaggcatac gcatcgcccg ggtgactgcg ccagtaaata
    18181 acaaaatatc ggcgtgacgc ggtgatgaaa caaccttaat gccaaaacgt tctgcgtcga
    18241 atacgggtgt gatggcggca aaaatttcga tttcacaggc gttgcagccg ccgcaatcga
    18301 cgcgataaac gtaggctgag cgacggatat cctgtagcaa atgccgcttc atcttttgcg
    18361 tttgctcgtc cagcgtgatg ggctggctga catgttgtgt aagcactgga ctcatagctg
    18421 ctccttagcc accagcggta catctgtatc gtcgttgatc agcgtcgcgc gttgtttgca
    18481 ctccgggcag acgctcgctt gcgcccgcaa catttcgcgg ttttgtggcg cattttgttg
    18541 ctgtgccaac aattcagcag ccagtgcgac ggttttttgc ggggcaaacg ggcgttcgca
    18601 acggctgcaa cgttgtagat ggaacgtcgc gcgggtatag agatcggctt tattggtgac
    18661 ggtcagttca aagttattgg taagctggat ggctctggtc gggcacactt cttcacaacg
    18721 tccgcagtaa atacaacgcc ccagatagag ctgccaggtg cgcgaatttt gctggtcgtc
    18781 ggtctggata gtcagcgcat ttgtcggaca agcacaggcg caggcaccac aggcaataca
    18841 ttggctgggc atcaggtccg gtttcccgcg aaagcctggg ctgacctcca gtggcgcgaa
    18901 gggatatttc accgtcgcgg ttccggcgcg cataatagtt ttcagtaact tcagcatctt
    18961 ctgtccttac ttcagcggcg aacggttacg atcaatgccg tagcgttcga tctctttata
    19021 cggcacggtt tttgactggc gcttgcgcac atcaaccagc gtcacacggt cggtacagga
    19081 gtagcaggga tcaaggctac cgataatcag cggtgcgtca gaaacggtat tgccgcgcag
    19141 catgtaacgc aacaccggcc agttggcgta ggtggcggca cggcagcgcc agcggaacaa
    19201 tttttggtta tcaccgagca tgctccagtg cacgtcttca ccgcgtggcg cttcaacaaa
    19261 gccgagcgcg aatgcgtgag gtttatagct aaagccttcg gtcagcagtg gggtatccgg
    19321 catgttgtcg agagcaaatt ccagcattgc cagcgaatca aacgtctctt tgacacggac
    19381 catcacgcgg gagaaaacat cgccgccggt gaaggtaaac agtgtttttg gaatattgcc
    19441 gtagtcggcg taggggtgat caaagcgcaa atcacgggca aaaccactgc cgcggatcag
    19501 cggccctaca gggctataat cacgggcgat ttgtcggtcg agaatgccaa tgccctgagt
    19561 gcgttgttcc atattcggcg tggcaagcag catttctacc agctccgaca cgtcggcgcg
    19621 catctcgcgc accagtttca gcgtttgcag acgttgctct ttgagaatat cgcggcgaac
    19681 accaccaatc agattcagac cgtaggtttt acgcgacccg gtcagcaatt ccgccattgt
    19741 catcgacttt tcccgcacgc ggaaaaattg cataaagccg gtatcaaatc caacaaaatg
    19801 gcaggagagg ccaaggttga gcaaatgact gtgtagccgt tcgacttcca gcagaatcga
    19861 gcgaatggta tgcgctcgtt gcggcacctc aatccccagt gcattttcaa ccgagttggt
    19921 ataggccaca ctgtgggcaa aaccgcaaat cccacacacg cggtccgata agaaggtcac
    19981 ttcgttgtag cccatccgcg tttctgccag tttctccatg ccgcgatgga cataaaacag
    20041 acggtaatca gcatcgacaa tttgctcgcc atccacaaac aagcggaagt gacccggttc
    20101 atcggaggtg atatgcagcg ggccgacagg gatcacccgc gcaccgctgt tgccctcatt
    20161 gataaacgga tacgtttcgg aatcagtcgt cggttcaggg cgcagtcgat aatccatcgc
    20221 atctttgcgc agcggatgca tatcttccgg ccagtcatcg ggcaacacca ggcgacgctg
    20281 atccggcagg ccaaccggaa tcagcccgta catatcacga atttctcgct cgccccagac
    20341 cgcggcaggg acgcgcggtg tgactgacgg aaactcccga ctgtcggcat cgaccagcgc
    20401 cttcaccaca atccagcatt tttcggcccc ttccattgaa agggcataat aaaccgcgta
    20461 atgaccgtta agtgtccgct cgtcgttgcc aaacaggacc ggaagccagc catcatgttg
    20521 ataataaaga tactgtacaa cgtcaggcag cagattgatt ttcaccgtaa tggtgacctg
    20581 ttcaggcgtt tgtcgctctt catccagcac cgcgccgggg aactgcgttt tcagggcggc
    20641 gagaatcgct tcgccacgat ttgacgatga attaacgttc actgtgtttc tccgaaacag
    20701 atgcggtgcc tgaaggcaaa aaatcatgcc aggtgctacg ttgtgcaggc aggtcgtgcg
    20761 tccctgagag gactatagtg gaagcgcccg ccaggatcct gatgacaggt tgtggaatat
    20821 gcgttcccat cgccagcatc atgaccagca gaatcaccat tggcgaggtg gtcaaccagc
    20881 cgagatcacc ccggttaacg gcctgcggcg gtttcgccat taacacccgc gcagccatcc
    20941 gtaccaggcc cgccagcacc agcgttaaca gcaataacag caggacgata atcagcaggt
    21001 gattacgtgc cagtccggcg gtaacggtca taaattcgct aagaaaaatg ttgaagggcg
    21061 gcatccctgc cagcgccagc gcaccgccgc caaacagcac ggcggtaaat ggcatgattt
    21121 tgagcatccc acagacgacg ttgagatcgc gcgtgccgta cttgagcagt acattgccgg
    21181 aaccgcagaa cagcagcgtt tttgccagac tgtggtttaa ggtgtgcagc agcgcggcaa
    21241 aaattcccag cggccggcca atgcctagcg ccaccgcgac cagtcccatg ttctccacgc
    21301 tggagtacgc cagcagacgc ttaatgtccc gctgtaccag aatgaaaaat gccgccacgg
    21361 caaccgacaa catgccgaag atgagcaaca accggttggg gaaatcgctg ccgatggcct
    21421 ggcaaataat gatgtaatag cgaatcagca ccaacagcgc gcagttcagc aatacggcgg
    21481 agagtagggc gctgaccgga ctcggcgctt cactgtgagc atccggcagc caggcgtgca
    21541 tgggaaatag cccggttttg gtaccaaagc caattagcac aaacacaaag gccaacagca
    21601 ttaatgttgg atcaagcaag gacgattgct taagaacctc gctccagaat atcgccattt
    21661 ctgcctgcgg cataacgctg gcggcgttgg cgtataccag cacggtaccg aacagaccaa
    21721 aagcgacacc aacagtacaa ataatgatgt acttccatgc tgcttccagc gatgaacgct
    21781 gaccgtaaat gcctaccaga aacgccgagc ttaaggtggt ggcttcgatc gccgcccaca
    21841 tcacaatcag gttattgctg gtaacaacca gcagcatggt gaacaaaaac agatggaaga
    21901 aaccgtagta atcgcacagc gtaacgggtg aaagctcgcc gtgctcaact tcatgacgca
    21961 tgtagccaat cgagtaaaca ccggtgagaa agccaatcac accaagaatg gcgaggaaca
    22021 aaccgcccag accatcaata tgaagccaca gtcccgcagc gaatatttct cctgcatcag
    22081 cggcagtttg gaccacccag agtgccagaa tcagcagcag tgtgatccct aagctatgta
    22141 atactgtcac cgtgcgagtc gcagagtgcc ctcgtttccg gcaggcaaaa cagagcagcg
    22201 aaaaaagcag cggagtgagc aggagtaaag cgaacatcac agaataactc atctcattat
    22261 cccttcagcg cggtcaagtt gttcacgtcc agcgtgccgt gggtacgcca tatttttctt
    22321 gccagtaaca ccatcacaat gacggcgaag atggcgtcgg tagcgatacc aatttccacc
    22381 agttccggtg cacgccaggc tagaagcgcc agcaccagat gggagccgtt ttccatcagg
    22441 cagtaaccaa aaatttgccg caggatattg cgctggctaa caatgcacag caggccaagc
    22501 agaaaatgac ctaacgccac cgccagcgct ggtttcagcc cggtagccat cggtagcttc
    22561 acgggctgaa cgacaaatgc gcaaagcagg acaattaacg ccgcgagcag tgccatcatt
    22621 gccggaccga ataacgcttt ttccgggatg ttctggggaa tatttcgtgc agcgtaagtc
    22681 atgattaacg gtaccagcag cactttggtg ataaaggcgc tggcggacca gatcagcagt
    22741 tgctctgcgg cgaacaggca cgagagggtg gcgaaaatag acaccagcac cagcgactgg
    22801 caggcgtaaa atccgcatga caggcgatag cttttgacgc taatcacaaa cagcgatgtc
    22861 agcatcatca gtcccgccag attatttacg atcatagaac cggtcattca tcatctcctt
    22921 actgcaacca ggtcgcggcg attacgctgg aaatcagcga catcacaatc agtacaatca
    22981 gcaccaggcg cattgaccct ggcagcggtg cggcatcggc gacggcctcg ctcggtttgc
    23041 cggggacgac gcgaccaaac cagcgaataa accaggcgaa actggcgacc gattcaatca
    23101 tcagcagaat catggcgggc agcaggatcc agtactccac tgacaacgca aaaccggcag
    23161 caaacagcgg gaatttactg aagaagccgt tgaacggcgg tacgccggta atcgccagcg
    23221 ctgccacgca gaaacccacg cctggcaacg gcagggtgtg caatacgcca cgcagacgcg
    23281 gcaacaagcg cgtgccgcag ctgtaactca gcgcacctgc tacaaggaaa aacaggcttt
    23341 tagcgaacgc gtggttgacg atgtaggcga tgctgccctc cagcgccagc cgcgagccga
    23401 agatggagag cgacaagccg aagaacatcc agccaagttg agtgatggtc gaccaggcca
    23461 gcaaccgctt catatcctgc tgtggcaaat acatcagaaa tccataaaga atggtgacca
    23521 gggccatgac catgccaacg ccgccaatca catgcgggat attgccgccg tcgataatgg
    23581 cgcgggcaaa aatgtaaacg cccactttca ccatcgatgc ggcgtggaga taggcgctga
    23641 tcggtgttgg cgcttccatt gcgtccggta gccacgcttg catcggtagc tgggccgatt
    23701 tcccccacgc ggcaaacagg atgccgccat aaaccagata acgtgcgtcg ccgtgtaact
    23761 cgctcatcgc gctaagcgca aacgttccgg tctgcaaaaa cagcgtggcg gcggcaagat
    23821 acaaccccaa cgaaccgata tgggtgataa gcaacgcttt tagtgctgaa cgctgcgctt
    23881 tatcgctctg ataataactg atcaacaccc aggagcagcc gcccgtaatt tcaaaaaaca
    23941 acaactgacc gagcagcgtc gaggagagta ccagtcccgc catcgcgccg ataaacacca
    24001 gcaaaaatgc gtaataacga ttcgtgccgt tatgcgggtg ttcgcgattt ttatccgtca
    24061 gataacccgt ggagtagatc gtgaccagca aaccgagaaa caccaccaca aacagaatca
    24121 gcgtacacac gcgatcaatg accaggccaa acaatgccac atcccccacg ttgaccaacg
    24181 tcaacgtgac ggcaacttta tcggcctgat aaaacgcgga gatcagcgac aacatgcaca
    24241 gcgtggtcag cgcggcgaac aaaacccccc attcggcggc ccgacgttgt ggcgaaaacg
    24301 aaacgaccag tgcgccgata aaaggcagca ataacgtcgt cagagcaaga ttttccatat
    24361 tccgtcagtg ctccttacag accggttaac cagaagaccc atgcaagcgc agcaaggctg
    24421 aagccaagcc aggtcacatg gtgaatgagt aaaaaacgcc cgcgtgccag cgtgttttct
    24481 gcgattgagg ccagtacaaa aatcagcaaa accttgagca gcgtaacgac aagtgaagtc
    24541 agcaggcagg cgagagaaag ttcttgcgcg cacccaaagg gcagaaacag ggccacaaac
    24601 agtgctgcca tcacgacctg tttcagcccc agcccccatt tcgccagcgc cagcccggca
    24661 ccggaatatt ctgtcagcgg gccttcctgt aattcctgtt ctgcttcagc aacatcaaag
    24721 ggaatttttc ccatctcaat gaagcaggca aaaccacagg ccagtaacgc cagtacggtg
    24781 gttagcggcg agttccagcc cgttgccagc gtattgctga tcatctcgat atgcgtggaa
    24841 cctgctatca gcgccaatac cagcagtgag agaatgagca ttggttcgac cagaatgccg
    24901 agcgtcaact cgcgactggc accgactccc gcaaacgggc ttccggtatc cagcccggaa
    24961 agagcaaaga aaaaacgaaa cagggcaaga agatagataa gggtgatcag atcgccgccg
    25021 cccgcaaaag gggaaacggt aataaatagt ggtagggcca tcgccagcac cagcatgctg
    25081 ctgattaata cccacggcat caggcggaac atcagacctg aagatatagg cgcaacttcc
    25141 tggcgtttaa acagtttgtg gatatcgcga taatcctgcc agatccccgg cccgcggcgg
    25201 gagtgcatac gcgcgcgtat ctgccgggaa atacccgtaa aaagcggggt tagcatcagc
    25261 agaatcacgg cctgtgctaa cgcaaaaatg accagatatc cgtcgcaaag agtttgtctc
    25321 atggtgattt ccttagacgg caatagcgat tagcagcaca accagtgcgg cgaccacata
    25381 caggcaatag agacgaaagt cgccgctttg cagatgctgt atttctttgg ccagccgttg
    25441 ggtggcgctc acgatggggc ggatcacccg ctcatcccag aagggttctg tgctctgagc
    25501 cctggcggtg acgtgcgcaa gacctttatt tagcctcagc gtagggtcga gctgttgtcg
    25561 tagacgaaat agcgcagaga agaccacacg cagcggctgc atcacgccat tgcctgacgg
    25621 ggccatcgca ttttcccagc cgtagccgca tgcccaggca tctcctgtgc gacgaaacgc
    25681 tgcgcggcgc gaacgacacc acagccagta aaggccaggc agcaaaggca tcgccagtag
    25741 taacagcaac agtaatgagg gggtgacctg tgtatgaaac gtgccgggta caagtgctat
    25801 tccgctggcg acagtgacgg gaggggtatt ggtaaacgca tgggcaatat gcattatctt
    25861 cggtgccagc caactggcac taagcgcaat taatacgcag agtgccgcga gcagtagcat
    25921 ggcgacgatc atcgtatttg gcacttcctg tgcctcttca gcgtgtgtac tgcgcggcgc
    25981 accacagaaa gtaataccgt acattttgac gaagcacatt actgccagcc caccagtgac
    26041 tgccagcatc accatagcaa taggacccgc aagttgtagc gctacggctt ccacacgact
    26101 tagtgagaac agcgactgcc aggtgtacca ttcgctgata aaaccattca gcggaggaag
    26161 ggctgatatc gcgaggcaac caatcaggca tgctgcggct gtccacggca tccgtttcgc
    26221 cagtgccccc attttttcca tgtcgtgggt atgcaaacgc gaaataatcg cgcccgcgcc
    26281 gagaaatagc agccctttga acagcgcatg gttgagcaga tgaaacagtg cgccgagcag
    26341 tccaaccacg gtgagcagcg ggtcgtgtag tgacagaccg accatcgcca caccgactgc
    26401 cagcaaaata atgccgacgt tttcgacggt actccaggcc agcagccgtt tgatatcctg
    26461 ttccgccagc gcatacagca cgcccaggag cgcggagatt gcgccgatcg ccatcaccag
    26521 aatgccccac cacagcggca aacccgtttg cgccagcaga tccatcgcca ctttcaggat
    26581 gccgaaaata ccgattttga ccattacgcc agacatcaac gccgaagcgt gcgatggtgc
    26641 ggcagggtga gcgcgcggca accagctgtg caacggcatc atcccggctt tcgcgccaaa
    26701 accgaaaaag gccagcagga acaccgccga cgccagcccc ggagaaagtg aaagcgtgcg
    26761 gaaactggca aaatctaggc tgccgctttc gcgccccatc agcaagaagg cgatcataat
    26821 cagcaccgat ccggcgtggg cgataaaaaa gtagagcatg ccagcgttga tcgacgtttt
    26881 atcctgcctg gcaatgacca gaaaccagga agacagcgac atcatttcaa acagcacgat
    26941 gaaccaaaaa gcgttgtcca tcaccagcag ggcaaccatc gatgcgatga aaatattcat
    27001 aaagaagccc atcgccgccg cgcctttgcc ctcgtattcg cgcatataag tcaatgaata
    27061 gagcgaacaa accaccacca gcaaggagat aaccagcacc ataaatgccg agaggctatc
    27121 cattcgcagg gttaaatcgg caaagtcaaa aggggtggca aaacgggcaa ctaaagttac
    27181 gccttcatgt aattgcgtaa ttccgctgat gatgccaatc acgccgccca ccagcgatgt
    27241 gatgccggaa agcttaatag ccagtctgtc cagaccgagt aaaaacagcg aagccagact
    27301 ggcaaacaga tagaggatca gcgaccaggt taataattgc agggcatcca ttagcgttgc
    27361 tcctgagtga gggaaagggg atcctccctg ccattggcaa accagcttgc ggcaaggcgc
    27421 tgtttttctt tcatctgacg ttgcaggcta tcgtcggtga tcagccgtaa cgcctgattc
    27481 gggcaagcgc gaacgcaggc aggaccttct ggcaagaaat cacacaggtc gcatttcacc
    27541 gcgacggtct ggacacccgg ttcccagcgc agcaaagcat gttgtgttgg cgcgttttct
    27601 tcgccgtctt tgagtgagcc ttcagcctga aaaacatatt gcgcaggggc attcaccgga
    27661 cggcttcctg aagcgctgat tgcgccaaat gggcagacca cggcgcaaag cttgcagcca
    27721 atacagaggc tttcgttgag ttggatcgca tcatccctct gggagatggc attgaccggg
    27781 cagacctgca cgcaaggggc ttcctcacag tgatgacaca cgacaggggc agtgagtgtt
    27841 gacgtcttcg ccagggccag gcgcgggtgt tgctgtaaac cttgcgtttt atgcacgtcc
    27901 gaacaggcag cgagacaggt attacatcct gtacaccaca gtggttcggc caccacaaag
    27961 cggttcatgt cggctccttg aaaatgatat gaaacattgc atttcataat gcataaagga
    28021 tgccagtatg attaatttaa tatattcaat aagttatgaa ttaactgaat tctgaacggt
    28081 acaaaaatga tggtcattaa cgttcagcaa aaaatggatt cgacacccgt gtcgcagggg
    28141 aaagtgagaa tgtgatcttg atcacgataa gtgaggtgtg ctgaccgcaa gcgcagccag
    28201 cacggaatgg agcaaagtaa tcaggcgtgt tctttcagcc agttcagcac aacgtcgtgg
    28261 tgattgctgg ttttgaaatc gtcaaagaca tgttcgattt tgccatcagc gtcaatcagg
    28321 aagctgatgc gatgaatacc atcgtaggtt ttgcccatga aggacttttc cccccagacg
    28381 ccgaattgct cgcacacctg gtggtcctca tcagacagga gcgtaaagtt aagcagctct
    28441 ttttccgcaa aacgggagag tttttcgggt ttatcggtgc tgatacccag cacatcaacg
    28501 cccgcttttt tcaactcatc catgttatcg cgtaagccgc aggcctgtac ggtacagccg
    28561 ggggtcatgg ctttcgggta gaaataaacc agaacacgct gtccctggaa gtcggtcaaa
    28621 ttaacttgtt ctccgtcttg atccggcaag ctaaatttcg gtgcgatatc accggctttc
    28681 agtggattca ttacttaact ccatcctgtt catcatgttg ggaataattg acgacgttaa
    28741 tactgccttg tgcattgagt tctgtacata gggctttgaa cgcttgctca atatttgctg
    28801 cgtccgcaga tgcggggctg tgggcagtaa tctgaatatg caactgcgca gcccgttcat
    28861 tttcagcagg ttgtgtgcgc gacaccagct ccgcaatgtt catatgatgc gcgtcgaaaa
    28921 gtgctgtgaa gcgttcaatt aaatgcgggg agtctgccac atcgacctga acccagacag
    28981 atgctggcat tggcggacgc ggacgcgccg tcgtgcgctt catcacgatt aaaagatcca
    29041 gttcggcacc tttcaacggt aacgttgatt caatcagagt aatggcattc catgaaccgg
    29101 aaagcagcat gataaacgtg aactcttctc ccagcatcgc caggcgactg tcttcaatat
    29161 tacagccgca actactgaca tgacgggtga tggtgttcac aattccaggg cgatcggcac
    29221 ccaacgcagt gatcaccaga taatgttgcg atgacagtgt caaactggtt attcctttaa
    29281 ggggtgagtt gttcttaagg aaagcataaa aaaaacatgc atacaacaat cagaacggtt
    29341 ctgtctgctt gcttttaatg ccataccaaa cgtaccattg agacacttgt ttgcacagag
    29401 gatggcccat gttcacggga agtattgtcg cgattgttac tccgatggat gaaaaaggta
    29461 atgtctgtcg ggctagcttg aaaaaactga ttgattatca tgtcgccagc ggtacttcgg
    29521 cgatcgtttc tgttggcacc actggcgagt ccgctacctt aaatcatgac gaacatgctg
    29581 atgtggtgat gatgacgctg gagctggctg acgggcgcat tccggtgatt gccgggaccg
    29641 gtgctaacgc tactgcggaa gccattagcc tgacgcagcg cttcaatgac agtggtatcg
    29701 tcggctgcct gacggtaacc ccttactaca atcgtccgtc gcaagaaggt ttgtatcagc
    29761 atttcaaagc catcgctgag catactgacc tgccgcaaat tctgtataat gtgccgtccc
    29821 gtactggctg cgatctgctc ccggaaacgg tgggccgtct ggcgaaagta aaaaatatta
    29881 tcggaatcaa agaggcaaca gggaacttaa cgcgtgtaaa ccagatcaaa gagctggttt
    29941 cagatgattt tgttctgctg agcggcgatg atgcgagcgc gctggacttc atgcaattag
    30001 gcggtcatgg ggttatttcc gttacggcta acgtcgcagc gcgtgatatg gcccagatgt
    30061 gcaaactggc agcagaaggg cattttgccg aggcacgcgt tattaatcag cgtctgatgc
    30121 cattacacaa caaactattt gtcgaaccca atccaatccc ggtgaaatgg gcatgtaagg
    30181 aactgggtct tgtggcgacc gatacgctgc gcctgccaat gacaccaatc accgacagtg
    30241 gtcgtgagac ggtcagagcg gcgcttaagc atgccggttt gctgtaaagt ttagggagat
    30301 ttgatggctt actctgttca taagtcgcgc ctggcaacgg ttgcgggtgt ttcgcttgtt
    30361 ttattactcg ctgcctgtag ttctgactca cgctataagc gtcaggtcag tggtgatgaa
    30421 gcctacctgg aagcggcacc gcttgcggag cttcatgccc cggctggaat gattttgccg
    30481 gtgacctccg gtgattatgc aatcccggtg accaacggta gtggtgctgt cggtaaggcg
    30541 ctggacattc gtccaccagc ccagccgctg gcactggttt ctggcgcgcg tacccagttc
    30601 acgggcgata ccgcttcatt gctggtggaa aatggtcgtg gcaatactct gtggccgcag
    30661 gtggttagcg tgctgcaggc gaaaaactac accatcaccc aacgtgatga tgctggtcag
    30721 acactgacca ccgattgggt acaatggaac cgtctggacg aagacgagca gtatcgtggt
    30781 cgttatcaaa tctctgttaa gccgcagggt tatcagcagg cggttacggt taaactgctg
    30841 aacctggaac aggcgggcaa accggttgca gacgcggctt ccatgcagcg ttacagcacg
    30901 gagatgatga acgttatttc cgctggtctg gataaatctg ccactgacgc cgcgaacgct
    30961 gcgcaaaatc gtgcctccac cactatggac gtacaaagtg cagctgatga caccggttta
    31021 ccaatgctgg tcgtacgcgg gccgttcaat gtggtttggc aacgtctgcc agcggcgctg
    31081 gaaaaagtgg gcatgaaagt gaccgacagc acccgttcgc agggcaacat ggccgtaact
    31141 tataagccgc tgtctgacag cgactggcag gaactgggcg cgagcgatcc aggcctggca
    31201 tctggtgact ataaactgca ggttggcgat ttagataacc gcagcagcct acagttcatc
    31261 gatccgaaag gtcatactct gactcagagt cagaacgacg cgctggtagc tgtcttccag
    31321 gctgcgttta gcaagtaaaa atacagggct ggaatcatcc ggcccttttt tctgatatga
    31381 tacgcaaacg tgtgcgtcgg caggaaaacg cgattttagc ggtaattcgc acgaaatttg
    31441 tttgtcggac gtagttcgga taaggcgttc acgccgcatc cgacaaaaca tccggcacac
    31501 cagacagcaa aagattttaa aacgttaatt cacacccagg agtgataaag atgcaaaagc
    31561 aagctgagtt gtatcgtggt aaagcgaaaa ccgtatacag cacggaaaac ccggacctgt
    31621 tggtgctcga attccgcaat gatacgtcag caggggatgg cgcgcgcatt gagcagtttg
    31681 atcgcaaagg tatggtgaac aacaagttca actacttcat tatgagcaaa ctggctgaag
    31741 cgggtatccc gactcaaatg gagcgtctgc tctccgatac cgaatgtctg gtgaaaaagc
    31801 tggatatggt gccggttgag tgtgtcgtgc gtaaccgtgc tgctggctct ctggtgaaac
    31861 gtcttggaat cgaagaaggt attgagctga acccgccgct gttcgatctg ttcctgaaaa
    31921 acgacgccat gcacgatccg atggtcaacg aatcttactg cgaaaccttt ggctgggtaa
    31981 gcaaagagaa cctggcacgc atgaaagagc tgacctacaa agcgaacgac gtgctgaaaa
    32041 aactgttcga tgatgctggt ctgattctgg tcgacttcaa gctggagttt ggtctgtaca
    32101 aaggtgaagt ggtactgggc gatgagttct ccccggacgg tagccgcctg tgggacaaag
    32161 aaacgctgga gaaaatggac aaagaccgtt tccgccagag cctcggtggc ctgatcgagg
    32221 cctatgaagc cgtcgcccgc cgcctgggtg tacagctgga ctgatttttc tgttcatcat
    32281 cttgccgtgc tgctggcacg gcaagacaac cattctcgta agatgtgcat tgaacgctca
    32341 ttcctccgcc atttcatccc gttttcctgt ggtaatcttc ctctgaacca cctacgatca
    32401 tttctataat gaatatattg ttgaggtatc tatgcgttgg caagggcgac gtgaaagtga
    32461 caatgttgaa gacaggcgca atagctccgg cggtccgtca atgggcggtc ccggtttccg
    32521 tctgccgagc ggtaaaggcg ggctgatttt actgatagtc gtgctggttg caggctacta
    32581 tggcgttgat ttaaccgggt tgatgaccgg gcagccggtt tctcaacagc aatcaacgcg
    32641 gtcaattagc ccaaatgaag acgaagccgc aaaattcacc tcggtgattc tggcaaccac
    32701 ggaagacacc tggggacaac agttcgagaa gatgggtaag acctatcagc aaccgaagct
    32761 ggtcatgtac cgtggaatga cgcgtaccgg atgcggggcg ggccagtcca taatggggcc
    32821 gttctattgc ccggcggatg gtacggttta tatcgatctc tccttctatg atgacatgaa
    32881 agacaaactt ggcgcggatg gcgattttgc ccaggggtac gttatcgccc atgaagtcgg
    32941 tcatcatgtg cagaaactgt taggcatcga gccgaaagtt cgtcaactgc aacaaaacgc
    33001 gacgcaggcg gaagtgaacc gcttatctgt gcgtatggaa ctccaggccg actgttttgc
    33061 cggtgtctgg gggcatagca tgcagcagca aggcgttctg gaaaccggcg atctggaaga
    33121 ggcgctgaac gcggcgcagg ccatcggcga tgaccgttta caacagcaaa gtcaggggcg
    33181 agtagtacca gacagtttca ctcatggcac ttctcagcaa cgctacagct ggtttaaacg
    33241 tggtttcgac agcggcgatc cggcacaatg caatactttt ggtaaaagca tttaacttcc
    33301 ggggcaggga tggctgaact gactgcgctt cacacattaa cagcgcaaat gaaacgtgaa
    33361 gggatccgcc gcttgctggt gttgagcggg gaagagggtt ggtgttttga tcatgcgctt
    33421 aagttacgtg atgccttacc tggcgactgg ctgtggattt cgccgcagcc agatgctgaa
    33481 aaccactgtt ctccctcggc actacaaact ttacttgggc gcgagttccg gcatgcggta
    33541 ttcgacgccc gccacggctt tgatgccgct gcctttgccg cacttagcgg aacgttgaaa
    33601 gcgggaagct ggctggtttt gttactccct gtatgggaag agtgggaaaa ccaacctgat
    33661 gccgactcgc tgcgctggag tgattgccct gaccctattg cgacgccgca ttttgtccag
    33721 catttcaaac gcgtacttac ggcggataac gacgctatct tctggcggca aaaccagccg
    33781 ttctcgttgg cgcattttac tccccgtact gactggcacc ccgcgaccgg cgcaccacaa
    33841 ccagaacaac agcaaatctt acagcagcta ctgaccatgc cgtcgggcgt ggcagcggta
    33901 actgctgcgc gtgggcgcgg taaatcggcg ctggcagggc aactcatttc tcgtattgcg
    33961 ggtagtgcga ttatcactgc gcccgcaaaa gcggcaacgg atgtactggc acaatttgcg
    34021 ggcgagaagt ttcgctttat tgcgcctgat gccttgttag ccagcgatga gcaagccgac
    34081 tggctggtgg tcgatgaagc cgcagccata cctgcgccgt tgttgcatca actggtatcg
    34141 cgttttcctc gaacgttgtt aaccactacg gtgcagggct acgaaggtac cggacgtggt
    34201 tttttgctga aattttgcgc tcgctttccg catttacacc gttttgaact gcaacagccg
    34261 atccgctggg cacagggatg cccgctggaa aaaatggtta gtgaggcact ggtttttaac
    34321 gatgaaaact tcacccatac accacaaggc aatatcgtca tttccgcatt tgaacaaacg
    34381 ttatggcgaa gcgagccaga aacgccgtta aaggtttatc agttattgtc tggtgcgcac
    34441 taccggactt cgccgctgga tttacgccgc atgatggatg caccagggca acatttttta
    34501 caggcggctg gcgaaaacga gattgccgga gcgctgtggc tggtggatga ggggggatta
    34561 tctcaagaac tcagtcaggc ggtatgggca ggttttcgtc gcccgcgggg taatctggtg
    34621 gcccagtcgc tggcggcgca cggcagcaat ccactggcgg cgacattgcg tggacggcgg
    34681 gtcagccgga tagcagttca tccggcgcgt cagcgcgaag gcgttgggca acagctcatt
    34741 gccagcgctt tgcaatatag gcctggcctc gactatcttt cggtgagttt tggttacacc
    34801 ggggagttat ggcgtttctg gcaacgctgc ggttttgtgc tggtgcgaat gggtaatcat
    34861 cgtgaagcca gcagcggttg ctatacggcg atggcgctgt taccgatgag tgatgcgggt
    34921 aaacagctgg ctgaacgtga gcattaccgt ttacgtcgcg atgcgcaagc tctcgcgcag
    34981 tggaatggcg aaacactccc tgttgatcca ctaaacaatg ccgtcctttc tgacgacgac
    35041 tggcttgaac tggccggttt tgctttcgct catcgtccgc tattaacatc gttaggttgc
    35101 ttattgcgtc tgctacaaac cagtgaactg gcattaccgg cgctgcgtgg gcgtttacag
    35161 aaaaacgtca gcgacgcgca gttatgtacc acacttaaac tttcaggccg caagatgtta
    35221 ctggtccgtc agcgggaaga ggccgcacag gcgctgttcg cacttaatga tgttcgcact
    35281 gagcgtctgc gcgatcgcat aacgcaatgg caattttttc actgactcct tcaattttct
    35341 cccatggtca ttgtgagtca gcggcgtaaa attaatgaca tcagttaagg agttcgccat
    35401 gaaacatgac cattttgttg ttcaaagccc ggataaacct gcgcaacagt tgttactgct
    35461 tttccacggt gtcggggata acccggtggc aatgggggaa ataggtagct ggtttgcacc
    35521 gttgttcccg gatgcgctgg tggtgagtgt cggtggcgcg gagccgagcg gtaatccggc
    35581 ggggcgtcag tggttttcgg tgcagggtat tacggaagat aatcgccagg cgcgtgtgga
    35641 tgccatcatg ccgacgttta ttgaaacggt gcgctactgg cagaaacaga gcggagtagg
    35701 ggctaacgcc acggcgctaa tcggtttctc tcaaggcgcg attatggcgc tggagagcat
    35761 taaagccgaa ccgggccttg cttcacgcgt cattgctttt aacggacgct atgcgagcct
    35821 gccggaaacc gcgtctacag ccacgacaat tcacttgatt catggcggtg aagatccggt
    35881 tatcgatctg gcgcatgcag ttgcggcgca ggaagcgtta atcagtgccg gtggtgacgt
    35941 gacgctggat atcgtggagg atttaggcca tgcgatcgac aatcgcagta tgcaatttgc
    36001 cctggatcac ttgcgttata ccattccgaa acattatttc gacgaagcgc taagcggcgg
    36061 taagcctggc gatgatgatg tgattgaaat gatgtaataa tagccgggta ggcctgataa
    36121 gaccgtatca ggcatcatgg ccggatgcgg cgttaacgcc ttatccggcc acttagctta
    36181 cttcttcggt tgatcctttt tcggccagtc gtcgtcatcg tcccacttat cgttgaaatc
    36241 acgatgcggg ggaagctccg gcttgttggc gagaaatttc ttatggtcga cgcgcttgag
    36301 atctttaatc acgttcagca ggacgcctac caaaaagaca atcaccagaa tccaccaata
    36361 tttagccagc cagtccatgc ttatttcctc ttgcagaacc actcatcacg cgacgagctg
    36421 ttccatgata cgttgataca tacgggcaag tagctgcagg tcggcagcgt tcacacattc
    36481 attaatttta tgaatagtgg cattgaccgg cccgagttcc accacctgcg cccccatgcg
    36541 ggcaataaag cgcccgtcgg acgttccgcc tgtggtcagt agctgcggtt taatttcatt
    36601 atagtgctca accgcgttaa cgaccgcatc caccagttta ccgcgcgcgg tcaaaaatgg
    36661 ctgcccggaa agccaccaat ccaccgtata gcgcagttga tgtttttcaa gcagggcaag
    36721 cacctgcgct ttgatcatct catcagtcag ttcggtgctg aagcggaagt taaactgcac
    36781 aaacagttca cccggaataa cgttgttact gcccgttccc gcctgaatat tggcaatctg
    36841 catactggtc gccgggaaga attcattgcc ctgatcccac tcaatagcca ctaattcatt
    36901 aaggaaaggt gctgcgcgat gtaccggatt gtcagccaga tgtgggtagg caacatgccc
    36961 ctgaacgcca tgaatggtta ggttgcaggt taacgatcca cgacgaccat ttttcaccac
    37021 atcacctacc acttcgatac tcgacggttc gccgaccagg cagtaatcga gacgctcatt
    37081 acgtgccatt aacgcttcga cggcttttac cgtaccattg tgggcactgg cttcttcatc
    37141 agaggtgatc agaaatgcca gtcgccctgt atggttggga tgttgtgcga caaaacgttc
    37201 tgcagctacc accatcgccg ccagcgagcc tttcatatct gccgcaccgc gcccgaataa
    37261 catgccgtca cgaatggttg gttcaaacgg cggattgatc caacgatcgg cgtcgccagg
    37321 cggcaccacg tcggtatgcc cggcaaaggc caatgtttca ccttgcccac gccatgccca
    37381 aaaattctgc gtatcggcaa agtccatgcg ttcaacggta aaaccgatcg cctgcaaacg
    37441 ttcaatcaac aaagcctggc atcctgcgtc atcaggactc agggaagggc ggcgaataag
    37501 ctgttgtgtc agctcaataa ccgggcacga catagactac acctcatgga aaaatttctg
    37561 ataactggaa tcactgaaac ccagcagcat aggcttacca ggcgcgcaga gcaatggacg
    37621 tttgataatt gcaggcattt cagtcattaa tgccgccgca gaggccgcat cggtgattat
    37681 attgcgggtg gtttcgtcca gtttacgcca ggttgtacca cgggtgttga gtaacgcttc
    37741 ccagcctaat tcgttgataa aaccgttcaa taattcgctg tccagcccat cgacgcggta
    37801 atcatgaaaa cgatagtcga tgttattggc ttccagccaa cggcgagcct ttttaatggt
    37861 gtcacaattt ttgatgccgt aaagtgtaac cataatgaat cctttttcga tttatgagaa
    37921 taaatactca tttaagagtg aaaacatttt gcctgcaaat gattatcagg aaatataaca
    37981 ttatcgaagt attcaaatta acagctatta aataatatta aacattttta acgtaaacat
    38041 taagaatatt catcttgtta tctgtctgta ttttaaccag aattaaatca gtattagatg
    38101 ccgtatgaat atctcgaaat gttgctgtgt atcacataca aatcatcact gcaggaccat
    38161 agtatttaca taaggtttgt cccccggagg tagtcatcgc agcaatcgag taaattagct
    38221 tcaccacggt atattgtttc cgtagaatac ccataattat aagagaggtt gttatgattg
    38281 aacgtgaact ggggaactgg aaagacttta ttgaagttat gcttcgtaag taattcctga
    38341 gacaaagtac aaacagcaag aacccgctga gcaggttctt aatcggaaag ttgaaaaagg
    38401 cgacacattg gcatgtcgcc ttttttattg cttattccgg gcgcggcttc agcgggaagc
    38461 ggcggcgcac cagcacaaag aacagcggca cgaagtaaat agccagaata gtggctgaaa
    38521 tcatcccgcc cattacgcca gtacccaccg catgctgacc accggaaccg gcacccgtgc
    38581 tggttgccat tggcaatacg ccgaagataa atgccagcga ggtcatcaga atcgggcgta
    38641 aacgctgacg gcaggcgtgg agcgtcgctt caaacaggtc gtggcctttt tggttcatct
    38701 cgttagcaaa ctcgacgatc aggatggcgt ttttcgccga taaaccaatg accgttaaca
    38761 ggcccacctg gaagtaaacg tcgttttcca gcccgcgcat ccaggttgcc agcagcgcgc
    38821 cgattacccc cagcggcacg accagcatta ccgagaacgg taccgaccag ctttcataca
    38881 atgcagccag acacaggaat accaccagca aggaaatggc gtacagcgcc ggagcctgcg
    38941 cgccggaaag ccgctcctga tacgacatcg ccgtccactc cagaccaaag ccgtttggta
    39001 gctgcttcac taacgattcc ataatatcca tcgccgtacc ggtactgacc cccggtgcgg
    39061 cttccccaac aatttcaacc gcagaataac cgttatagcg ttccagacgc ggcgagcctg
    39121 tctcccagcg tgaggtcgcg aaagcagaga agggcaccat gccgccatct ttatttcgga
    39181 cataccagag attgatgtca tctggcagca tgcgatacgg cgcagctgcc tgcacataga
    39241 ctttcttcac gcgaccgcga tccataaagt cattcacata gctcgaaccc caggcggttt
    39301 gcagtgtgtc gttaatatcg tcgatagcaa cgcccagcgc ctgagcttta cgctggtcga
    39361 tatcaatctg caactgcgga ctgtcatcga ggccgttatg gcgcacacgg gttagctccg
    39421 ggttttccgc cgccagcgcc agcaactggt tacgtgctgc catcagcgca tcgtgacctg
    39481 ctccagcgtg gtcctgcaac tccatatcaa aacctgcaga actaccaaga ccgctaattg
    39541 ctggcgggct gctggcgata acgcgagctt ctttaatttg gttaaacgcc tttgttgcac
    39601 gctcgataat ggcaaacgag gtgccggtct tactgtcgcg ttcgctccag tctttcaggc
    39661 ggataaacat tcgcgccacg ttttgcccgt tacccccagg gccagaacca acagtggcaa
    39721 acaccgacat gatgttgtct ttttcatggg tgaagtaata tttctcgatt tgctcaacga
    39781 ctttcagggt ctgttgttgg gttgaaccgc tgggcaattg taccgaggta gtaaacatgc
    39841 cacggtcttc cagcggtaag aacgacgtcg gcaaacgcag gaacaggaac accatgccgc
    39901 caagcagcag gacataaatc acaatccagc gcaggctacg gtggagaatt ttcgccaccc
    39961 ctttttcgta gcgttcggcg ttgcggttaa acatctggtt aaaccaggca aaaaagcctt
    40021 tttgcccgtg atgctcacct tttttcagtg gcttaagtag tgtggcacac agagccggag
    40081 tgaggatcat cgctaccagt actgacagca ccatcgccgc aacaatggta atagagaact
    40141 ggcgatagat ggcaccggtg gtgccgccga agaaggccat tggtacaaat accgccgaaa
    40201 gcaccatcgc aatcccgacc agtgccccct ggatctgccc catcgattta cgcgtggctt
    40261 cgcgaggagt gagtccttcc tcactcataa tacgttcgac gttttccacc accacgatgg
    40321 cgtcatccac cagcagaccg atcgccagca ccatcgcgaa catggttaag gtgttgacgc
    40381 tgtaaccgaa ggcgtaaagt acggagaagg ttcccatcaa caccaccggc acggcgatag
    40441 tgggtatcag cgtggcgcgg aagttttgca ggaacagata cataacgagg aaaaccagag
    40501 cgatagcttc cagcagcgtt ttcaccacgt cttcaatgga ggctttaaca aacgaggtgg
    40561 tttcatacgc caccttgtat tccagtccgt gcgggaaata ctgcgccagc tcgtcgagac
    40621 gattgagcac cagctccgct gtcgccattt cgttcgcgcc ggaggccagt tttaccccca
    40681 acccggaggc tggcttacca ttgaagcggc taagataatc gtatttctcc gcccccattt
    40741 cggcggtggc gacatcgccg agccttactt ctgagccgtc ctgattcacc cgcaaggtga
    40801 tatcgcggaa ctgttctggt gtttgcagca gtgactgggc gttaatggtg gcgttgagcg
    40861 cctgcttatc gacggaaggt gtaccaccaa gttgcccaac cgcaatctgc gcgttctgtg
    40921 actcaatggc atcagtgaca tctttagctg tcatctggaa actgttgagt ttcgccgggt
    40981 ccagccagat acgcatggaa tattgcgaac cataggcatc gatatccccg acgccattta
    41041 cgcggcttaa cgggtcctga atattactgg caacataatc agcaatatcc tgtttatcca
    41101 tcgaaccatc ggtagagacg aaggcaatgg tcagaatgtt ggtatcgccg gttttacgca
    41161 cagtcacgcc ctgattttgc accgcctgcg gtaactttcg catggctgat tgcagctggt
    41221 tttgtacttg ctgcacggct tcgtccggat cggtgcctgc tttaaaactt aaagtgacag
    41281 atgcctgacc ggtgccactg ctctgagatg acatatacat gagattatcg aggccggtca
    41341 tattttgctc gataacctgg gtcacggtgt tttccagcgt ctgggccgat gcgccgggat
    41401 agttagcggt cactcgcaca ttcggtggcg cgagatcggg gtattgttca acgggcaatg
    41461 aaaaaatcgc cagggtacct gtcagacaca acaggattgc cagcacccag gcaaaaatgg
    41521 ggcgatcaat aaagaaattc gccattaaaa gaggacctcg tgtttctgcg tatcgttgta
    41581 ggcttgactt gcttcactgt agcggcaagg tacgagccaa tcgtggagaa aaaaaggagt
    41641 taatgtaaat tatcatgccc ttttatactg aaccttaact gcaagtattc tttggtcagt
    41701 aggaggcatt acatcaactg actttcctca ccctccgcag agcgaaagct aatactcact
    41761 aacgtgccgc cgccggaagg ttgtgaaaaa gtcagcgtcc cacctagccg ttccgcgcgt
    41821 tcgcgcatga tattcagacc ataatgacct tcgggttctt tcggttcacc gataccaatc
    41881 ccgttatcac ggatataaac cgtgtgattg ccgtccggcg cggtgacgca actgacggcg
    41941 atttcgctgg cgttggcgtg cttcatcgca ttcagcaccg cttcgcgaat aatttgcaac
    42001 aaatgcacct gcatttgcgc atccagtgcc agggttggca gacggcagtc gagggtcagt
    42061 ttggcgctgg tttgattttg taacgtatcc agcatttccc tcaacgcgga ggggagatcc
    42121 gcctgctgca gcgtcaggcg aaaagtagtc aacagctcgc gtaactgccg ataagcatca
    42181 ttcaacgcct gggaaaaatc ggccatgata ctttgtgcgg tggcgttatc ttccggtatc
    42241 gaacgcttaa gtaacgtcaa ctggatacgt aagtaagaaa gtacctgagc cagcgagtcg
    42301 tgcaattcgc gggcgatggt tgcacgttct tccatcaaca ataactgctg aaaatgcttc
    42361 tgcgcctgat taaagtacaa accgcgtccc agcatcgacg aaacgctgtt aagcagcggt
    42421 tctgatgatg aaacgtgact attttgccag tgcagttcgc cgtaaaccgt ctcttgcatt
    42481 gtcaccggta aaatctgcat cggcaattcc gggtttggtt gcccttcgct aatccgccag
    42541 ttttcaccga catttaactc cagatattca gccgcttcat tgtcgcgaac aatctgcaaa
    42601 atatggcgga aacaatgcac atcaatctgg ctggtgttca gcgcctgcga acattgatac
    42661 aacacctcca gacgacgctt ggcctcgtgg agatcgcggg tcttttcttc tactgacgcc
    42721 tccagcgaac ggtacaattt atgcagctcg ctcgacatct ggttaaaggt ttttgcaagt
    42781 agaccaagct cattcggcag gctggtatcc agcggtggcg agtcgaactg tccgtgttca
    42841 atacgctgac tggcggtaac cagctgattc agcggggcaa ccacctgatg gcgtatgcgg
    42901 cccaaagtaa aaaagaccag cgtgaaaata ccgataccgc cagccaggga aatcgccacc
    42961 accagcagca ttttgcgttc agcgtagtgc tgtaaagcca gcacgaacag gtctatctga
    43021 ttaacataat tattaatatt ggcctgatac cacggcaaat cgcccttgct gagccgatta
    43081 ttcatctcca gccagttggc attcagatgc gcatagcgag tttttactgc ttctggcaca
    43141 taccagacgt tgaggttggt taataccggt gaatgcagtg cctgctgaaa cagctggcga
    43201 tgtgcattga gttgtggagt gccactttgc aagtcgtagc ccaggcggta actctgcata
    43261 cgcagcgatc cggcaatatt gatagcctca gcgtcgcgca aactgctcgc cagagttagc
    43321 agagcgatac ccgtggaaag aatcgacagc agcacaatgt aaaaaaaggc ccgggccaga
    43381 ctggccgaga cgggtcgttt aacaatcaca cgcgtcttct ccacaaaaaa tcattaatgt
    43441 tccagttcgc tgaaacgcaa tttagttagt gatgggatta tttgatctgc cacagtttat
    43501 agcgaaatag ttacttattc tgggcacatg aacattgccg attcccctgt aagtcgggta
    43561 ataacaacac tcatataaag aataaggttt ttacaaccaa aaaagaaggt cgttatgaat
    43621 cgttttatta tggccaacag tcagcaatgt ctgggttgtc atgcttgtga aatcgcttgt
    43681 gtcatggctc acaatgatga gcaacatgtc ctgagccaac accattttca tccccgaatt
    43741 acggttatca aacatcagca gcaacgtagt gcagtgacct gtcaccattg tgaagatgcg
    43801 ccctgcgccc gtagctgccc taatggcgca atcagccacg ttgatgacag cattcaggtc
    43861 aatcagcaaa agtgtattgg ctgtaaatcc tgcgtggtgg cctgtccgtt tggtacgatg
    43921 caaatcgtcc tgacacccgt cgcagcgggc aaagttaaag ccacggcgca taaatgcgac
    43981 ctttgtgcag ggcgcgaaaa cggtcctgcc tgtgtcgaga attgcccggc ggacgcgctg
    44041 cagctggtca ctgacgccgc actctcaggc atggcgaaat cccgccgctt gcgtaccgca
    44101 cgtcaggaac atcaaccgtg gcatgccagt accgctgcac aagaaatgcc ggtaatgagt
    44161 aaagtcgaac aaatgcaggc aacgcccgcg cgtggcgagc cggataaact ggcgattgaa
    44221 gcgcgcaaaa ccggttttga tgaaatttat ctgccattcc gtgccgatca ggcacaacgg
    44281 gaagcctcgc gctgccttaa atgcggcgag cacagcgttt gtgaatggac ctgcccgctg
    44341 cataaccata taccgcagtg gattgaactg gtgaaagccg gaaacatcga cgccgccgtc
    44401 gagctttctc acctgaccaa caccctgccg gaaattaccg gacgcgtttg tccgcaagac
    44461 cgtttgtgtg aaggtgcctg tactattcgc gatgagcacg gtgcggtaac tatcggcaac
    44521 attgaacgct acatttcaga tcaggcgttg gcgaaaggtt ggcgtcctga cttaagccat
    44581 gtcaccaaag tggacaagcg ggtggcgatt atcggtgcag gtccggcagg gctggcctgt
    44641 gcggatgttc tgatccgcaa tggcgtggcg gtgacggtgt acgatcgcca tccagaaatc
    44701 ggtggcttgc tcactttcgg cattccttct ttcaaactgg ataaatccct gctggcacgt
    44761 cgtcgggaaa tcttcagcgc gatggggatt cacttcgaac tcaattgtga agtgggtaaa
    44821 gatgtctctt tggattcgct tttggaacaa tacgacgcgg tcttcgttgg cgtaggcact
    44881 taccgttcca tgaaagcggg tttacccaat gaagatgcgc cgggcgttta tgacgcgctg
    44941 ccgttcctca tcgccaacac taaacaggtg atggggctcg aagagctacc ggaagagccg
    45001 tttatcaata ccgccggact taacgtcgtg gtactgggcg gcggcgacac cgcgatggac
    45061 tgtgtgcgta ctgcgctgcg ccacggcgcg agtaacgtca cctgcgctta tcgtcgtgat
    45121 gaagctaaca tgccaggctc gaagaaagag gtgaagaacg cccgcgaaga gggggccaac
    45181 ttcgaattta acgtccagcc ggtggcgctt gagctgaatg aacaaggtca cgtctgcggg
    45241 attcgtttcc tgcgcacgcg tcttggcgag ccggatgccc aggggcgtcg gcgtccagtg
    45301 ccggtggaag gcagtgaatt tgtcatgcca gccgacgcgg tgattatggc gtttggcttc
    45361 aatccgcacg ggatgccgtg gctggagtcg cacggtgtaa cggtagacaa atggggccgc
    45421 atcatcgcgg atgtggaaag ccagtaccgt taccagacca ccaatccgaa aatcttcgcc
    45481 ggtggcgacg ccgtgcgtgg tgcggatctg gtggttaccg caatggcaga aggacgtcat
    45541 gcggcacagg ggattattga ctggctgggg gtaaaatcag tcaaatctca ctgatagcct
    45601 gcgcagacaa acccgacttc acagcgtaat ataattgttc atttcgcgct gtggagtcgg
    45661 tatgacgcaa caaatcaccc tcattaaaga caaaattctc tccgataact atttcaccct
    45721 gcacaacatt acttacgatc tcacgcgcaa agatggcgaa gttatccgcc ataaacgtga
    45781 agtttacgat cgcggtaatg gtgcgacgat cctcctgtac aacgcgaaga aaaagaccgt
    45841 ggttctgatt cgtcagttcc gtgtcgctac ctgggttaat ggcaatgaaa gcgggcagct
    45901 gattgaaacc tgcgccgggc tgctggataa cgacgaaccg gaagtgtgca ttcgcaaaga
    45961 agcgattgaa gagacgggct atgaagtggg cgaagtgcgc aaattatttg aactgtatat
    46021 gtcgccaggc ggtgtgactg agctaatcca cttttttatc gccgaataca gtgacaatca
    46081 gcgcgctaac gccggtggcg gtgtcgaaga tgaagatatt gaagtgctcg agctgccgtt
    46141 cagccaggcg ctggagatga tcaaaaccgg cgagatacgt gacggtaaga cggtgttatt
    46201 gcttaactat ttgcaaatgt cacatttaat agactgaaaa ataacaataa tatttcgttg
    46261 tttattcttg gctcaatccg acaaatccga ttgagccgcg ctacctgcgc aacgaagata
    46321 cgacttgtgc tgtttgtttg aacttctggg gtcgtaccgt ccatgcgcta tcgcattttc
    46381 cttctctttt ttttcgcttt gttgccgacg tctttggtgt gggcggcacc agcgcaacgg
    46441 gcgttttccg actggcaggt cacctgtaat aaccaaaact tctgcgtggc gcgtaatacg
    46501 ggcgatcata atggactggt gatgaccctg agccgcagcg ccggggcgca taccgatgcc
    46561 gttttacgta ttgagcgcgg cgggttgaag tcgccggatg cgtctgaagg ggaaattgcg
    46621 ccacggctgc tgttagatgg cgagccgttg gcgttaagtg gtgacaagtg gcggatttca
    46681 ccatggttat tagtaaccga tgatacggca accatcaccg cgtttttgca gatgattcag
    46741 gaagggaagg cgatcacctt acgcgacggc aatcagacca tttctctgag tgacttaaaa
    46801 gcggcgttgt tgtttattga tgctcagcaa aagcgcgttg gcagtgaaac cgcgtggatc
    46861 aagaaagggg acgaaccgcc gctcagcgta ccgcccgcgc ctgcgctgaa agaggtcgtg
    46921 gtggttaacc caacgccgac gccactctca ctcgaagaac gcaacgattt gctggattat
    46981 ggaaactggc ggatgaatgg tctgcgctgc tcgcttgatc cattgcgtcg tgaggtgaat
    47041 gtcactgcgc tgactgatga taaagcgctg atgatgatta gctgtgaggc aggggcctat
    47101 aacaccattg atttggcatg gattgtgtcg cgtaaaaagc cgctggcctc gcgcccggtc
    47161 cggctgcgtt tgccgttcaa caacggtcag gagacgaatg aactggaact gatgaacgca
    47221 acatttgatg agaaatcgcg tgaactggtg accttagcga aagggcgcgg attaagcgat
    47281 tgtggcattc aggcgcgctg gcgctttgac ggtcagcgtt ttcgcctggt gcgttacgcc
    47341 gcagaaccca cctgcgataa ctggcatggg ccagacgcct ggcccacgtt gtggatcacc
    47401 cgttaaacaa aatggcggga catgtaggcc ggataagacg ctgtaggccg gataagacgc
    47461 gtcagcgtcg catccggcaa tcagcatccg gcgatcacca tcaggcacct tttactccca
    47521 gcaccttatg cgcttttgcc acaatattct cggcggtaaa gccaaagaac gggaacagct
    47581 tatccgccgg agcagattcc ccataacccg tcatcccgac aattgcccct ttcagaccaa
    47641 catacttgta ccagtaatcg gcaatacctg cttccaccgc cacgcgagcc gcaacgttag
    47701 aaggcaacac cgactcccga tattcctcat cctgggcgtc gaaaatatcg gtcgagggca
    47761 gggaaaccac gcgaacattg cgaccttctc ccgctaattt ctccgccgct tgcagggtga
    47821 tttccatctc tgaaccggtg gcaatcaaaa taatatctgg cttaccgccg ctgtctttca
    47881 gcacataacc gccacgagca atctctttaa cctgatccgg cgtacgttcc acctgggcca
    47941 gattctgcct ggagagaatc agtgccgtcg gtccgttgtg gcgctcaacc gccagcttcc
    48001 agcccaccgc cgcttccacc tgatcgcacg gtcgccaggt gctgaaattt ggcgttaagc
    48061 gcaggctggc cagttgctca acagcctggt gcgtcggacc atcttcgccc agaccaattg
    48121 agtcgtgggt ataaaccata atctgccgcg ctttcatcag tgccgccatc cgcgcggcgt
    48181 tacgggcgta ttcaacaaac atcaggaacg ttgcggtata cggcacaaag ccgccgtggt
    48241 gcgcaatgcc gttggcgata gcggtcatgc caaattcacg caccccgtag tgaatgtagt
    48301 tgcccgccgg atcttccttc agcgaaacag aacctttcca gatggtcagg ttgctgggag
    48361 ccagatccgc cgaaccgccg agcagctccg gtagcatcgg cccgtaagcg ttaagcgtat
    48421 tttgcgaagc cttacgggta gcgattttcg ccggattcgc ctgcaactca ttgatatatt
    48481 tctgagtcgt tttctcccag tccttcggta aaccaccgct catccgacgg gtaaactctt
    48541 ctgccagttg cggatgagcc tttttatagg cggcaaactt ctcattccag ctctgctgcg
    48601 ctttttcgcc tttttcgcgg gcatcccaag cgtgataaat atctttaggg atctcaaatg
    48661 gcggatggtg ccagcccagt ttttgccgtg ccagcgccac ttcttcttcc cccagcggtg
    48721 cgccgtgcgc ctcttcctta cctgctttat tcggcgaacc aaagccaatc accgtacggc
    48781 agataatcag cgacggctta tctttcacgc tttgcgcttc aaggattgct tccttcaccg
    48841 cctgcggatc gtgaccgtcg atttcatgga tcacatgcca gtgataggct tcaaaacgtt
    48901 ttgccgtatc gtcggtaaac cagccttcgg tttctccatc aatcgaaata ccgttgtgat
    48961 cgtaaaaacc aatcagcttg cccagtccca gcgtgcccgc cagcgaacag acttcgtggg
    49021 aaataccttc catcaggcag ccgtcgccca taaacacata ggtgaagtga tcgacaattt
    49081 catgatccgg ctggttaaac tgcgccgcca gtgtacgttc cgctatcgcc agcccgacgg
    49141 cgttcgccaa accttgtcca agaggaccgg tggtggtttc aactccgggc gtatagccga
    49201 tttccgggtg gccaggggtt ttcgaatgca gttgacgaaa gttcttcagc tcttccagcg
    49261 gcaggtcgta accggtcaga tgcagcaaac tgtagagcag catcgacgcg tgaccgttgg
    49321 aaagaataaa gcggtcgcga tcataccagg ttgggtcggt agggttatgt ttaagaaaat
    49381 cgttccacag cacttcggca atatcagcca tgcccatcgg cgcgccggga tgaccagaat
    49441 tggctttttg taccgcatcc atactgagtg cgcgaatcgc attggcaagg tcttttcggg
    49501 acatataaca ctccgtggct ggtttatagt ttggcggcaa gaagatcttc cagtttgcgt
    49561 tgatcaacgg cgaacagacg aatgccttca gacagttttt ctaccgccat cgcatcctga
    49621 ttgtgctccc aacggaactc cgcttcgctc atgggagctg ggcgtgggaa cgtctgagaa
    49681 ggtgggatta atttacgtac cactggcgaa actttttcct gcagctcctt cagcaaattc
    49741 ggtgcgatag tcagtcgatc gcagccggtt aaggcgagga tttgttcggt gcgacggaag
    49801 ctcgcgccca tcacaatggt ttcatagtgg tgttgcttat agtagtcgta gatattgcgc
    49861 accgatttaa cgcccggatc ttcttccacc acatatgggt ccatcggctt gcgtgcctga
    49921 taccagtcat aaatacgccc gacaaacggc gaaatcagaa aaacgcccgc ttctgcacag
    49981 gcccgcgcct gtgcaaaaga aaacagcagc gtcaggttgc agttaatacc ttctttttcc
    50041 agctcttctg ccgcgcgaat tccttcccag gtcgaagcca gtttgatcag aatgcgtgat
    50101 ttctcaacgc cttgttgctg atacaagtcc accagatggc gcgccttctc aatactcttt
    50161 tctttatcaa aagagaggcg tgcatcaact tctgttgaca cgcgaccggg tacgattttg
    50221 aggatttcag caccgaaatt gaccgccagt ttgtcacacg ctgcgaccac ctgttgttcc
    50281 tgggtcttgc catttttttt accccaggcg atagcatcgt ctattaaatg ctcatattgt
    50341 gataatccgg cagccttgag taacagcgaa ggattggtgg tggcatcctg gggatgataa
    50401 tggcgaatgg actcaatatc gccgctgtct gccacgacag tggtgaactg tttgatgccg
    50461 tctaactcgt tcataggaaa tactccttga aaagtaaagt gttagatgag tgcgttaatt
    50521 cacacttctg agaaatttcg ctaaacgcat caaaaaagca tagcagacag gcatggtatt
    50581 gctggattaa gcaggtaaca tcagtgttat aggattatta ccaaaacatt atattaatgg
    50641 tgagagtttg gacttgctca aagtctgtag actccggcaa ggtaataatg tgcgccacgt
    50701 tgtgggcagg ggctgttgcc cacacacttt atttgtgaac gttacgtgaa aggaacaacc
    50761 aaatggatga ccagttaaaa caaagtgcac ttgatttcca tgaatttcca gttccaggga
    50821 aaatccaggt ttctccaacc aagcctctgg caacgcagcg cgatctggcg ctggcctact
    50881 caccaggcgt tgccgcacct tgtcttgaaa tcgaaaaaga cccgttaaaa gcctacaaat
    50941 ataccgcgcg cggtaacctg gtggcggtga tctctaacgg tacggcggtg ctggggttag
    51001 gcaacattgg cgcgctggca ggcaaaccgg tgatggaagg caagggcgtt ctgtttaaga
    51061 aattcgctgg gattgatgta tttgacattg aagttgatga actcgacccg gacaaattta
    51121 ttgaagtcgt cgctgcgctc gaaccgacct tcggcggcat caacctcgaa gacattaaag
    51181 cgccagaatg tttctatatt gaacagaaac tgcgcgagcg gatgaatatt ccggtattcc
    51241 acgacgatca gcacggcacg gcaattatca gcactgccgc catcctcaac ggcttgcgcg
    51301 tggtggagaa aaacatctct gacgtgcgta tggtggtttc cggcgcgggt gctgcagcaa
    51361 tcgcctgtat gaacctgctg gtagcgttgg gtctgcaaaa acataacatc gtggtttgcg
    51421 attcaaaagg cgttatctat cagggccgtg agccaaacat ggcggaaacc aaagccgcat
    51481 atgcggtagt ggatgacggc aaacgtaccc tcgatgatgt gattgaaggc gcagatattt
    51541 tcctcggctg ttccggcccg aaagtgctga cccaggaaat ggtgaagaaa atggctcgag
    51601 cgccaatgat cctggcgctg gcgaacccgg aaccggaaat tctgccgccg ctggcgaaag
    51661 aagtgcgtcc ggatgccatc atctgtaccg gccgttccga ctacccgaac caggtgaaca
    51721 acgtcctttg cttcccgttc atcttccgtg gcgcgctgga cgttggcgca accgccatca
    51781 acgaagagat gaaactggcg gcggtacgcg cgattgcaga actggcccat gcggaacaga
    51841 gtgaagtggt ggcttcagcg tatggcgatc aggatctgag ctttggccct gaatacatca
    51901 ttccaaaacc gtttgatccg cgtctgatag tcaagattgc tcctgcggtc gcgaaagcgg
    51961 cgatggagtc gggcgtggcg acgcgtccga ttgctgattt cgacgtctac atcgacaagc
    52021 tgactgagtt cgtttacaaa accaacctgt ttatgaagcc gattttctcc caggctcgca
    52081 aagcgccgaa gcgcgtagtt ctgccggaag gggaagaggc gcgcgttctg catgccactc
    52141 aggaactggt aacgctggga ctggcgaaac cgatccttat cggtcgtccg aacgtgatcg
    52201 aaatgcgcat tcagaaactg ggcttgcaga tcaaagcggg cgttgatttt gagatcgtca
    52261 ataacgaatc cgatccgcgc tttaaagagt actggaccga atacttccag atcatgaagc
    52321 gtcgcggcgt cactcaggaa caggcgcagc gggcgctgat cagtaacccg acagtgatcg
    52381 gcgcgatcat ggttcagcgt ggcgaagccg atgcaatgat ttgcggtacg gtgggtgatt
    52441 atcatgaaca ttttagcgtg gtgaaaaatg tctttggtta tcgcgatggc gttcacaccg
    52501 caggtgcaat gaacgcgctg ctgctgccga gtggtaacac ctttattgcc gatacctacg
    52561 tcaatgacga accagacgca gaagagctgg cggagatcac cttgatggcg gcagaaactg
    52621 tccgtcgttt cggtattgaa ccgcgcgttg ctctgttgtc gcactccaac tttggttctt
    52681 ctgactgccc gtcgtcgagc aaaatgcgtc aggcgctgga actggtcagg gaacgtgcgc
    52741 cagaactgat gattgatggt gaaatgcacg gcgatgcagc gctggtggaa gcgattcgca
    52801 acgaccgtat gccggacagc cctttgaaag gttctgccaa tattctggtg atgccgaaca
    52861 tggaagctgc ccgcattagt tacaacttac tgcgcgtttc cagctcggaa ggtgtgactg
    52921 tcggtccggt gctgatgggc gtggcgaaac cggttcacgt gttaacgccg atcgcatcgg
    52981 tgcgtcgtat cgtcaacatg gtggcgctgg ccgtggttga agcgcaaacc caaccgctgt
    53041 aattcttttt taactctcac gcttaccctg aatattcagg gtaagcggtt tagctgcaat
    53101 atattagtaa agcttattac tgagtttgcg aataataaaa aaagcagtat ataaaatatc
    53161 tcgatattat tttatttata ttcatgcgtc gcatatgaaa gtttattcat aacagtaaat
    53221 atctctccat tcttagtgat ctacctcacc ttttaaacgc gcttgccgaa ttttgttatt
    53281 tactctgacg aaaaattgtc acgatacacg aaagtttttc acaggcggcg actcatggat
    53341 aaagaacgca tcattcagga atttgtgccc ggcaaacagg tcacactggc gcatctcatc
    53401 gcgcatcccg gcgaggaact ggcgaagaaa atcggcgttc cggatgcggg cgcaattggc
    53461 attatgacgc taactcccgg cgaaacggcg atgatcgccg gtgatttagc gctgaaagcc
    53521 gccgatgtgc atatcggatt tctcgaccgc ttcagtggtg cattggtgat ttatggctcg
    53581 gtgggcgcag tagaagaagc gttatcacaa accgtcagtg gtttaggtcg cttattaaat
    53641 tatacgcttt gtgaaatgac caaaagttaa tacgaggcat tcatgaaacg aattgcgttt
    53701 gtgggtaccg tcggggcagg gaaaacaacg ttatttaatg cgttgcaggg gaattatacc
    53761 ctcgccagaa aaacacaggc cgtggaattt aatgataagg gcgatattga tacgccgggt
    53821 gaatatttta gccatccccg ctggtatcac gccttaatta ccacgctaca ggatgtggac
    53881 atgctgatct atgtccacgg cgcgaatgac ccggaaagtc ggttgcctgc cgggttgctg
    53941 gatattggcg tcagcaaacg gcaaatcgcc gtcatcagta aaacggacat gccggatgct
    54001 gacgtcgccg caacgcgaaa gttattgctt gagacgggct tcgaggagcc gattttcgag
    54061 ctaaacagcc acgacccgca aagcgtgcag caactggtgg attatctggc atctctcacc
    54121 aaacaggagg aagcaggtga aaaaactcat cacagcgaat gatattcgtg aagcacacgc
    54181 gcgcggcgaa caggcaatgt ccgtggttct gcgcgccagc attattaccc cggaagcccg
    54241 tgaagtggcg gatctactgg gttttaccat caccgaatgt gatgaattga tcccggtaac
    54301 ggcgtccgtg cccgccagcg tgccagccga taaaaccgaa agccagcgca tccgcgaaac
    54361 cattatcgcc cagctaccgg aaggccagtt taccgaaagc ctggtcgcgc agctgatgga
    54421 aaaagtgatg aaggaaaaac agtcgctgga gcagggtgca atgcagccga gcttcaaatc
    54481 ggtgaccggc aaaggcggca tcaaagtgat tgatggtagc agcgtcaagt ttggtcgctt
    54541 tgatggcgca caaccgcatt gcgtgggctt gactgatctt gtgaccggag acgacggcag
    54601 cagcatggcc gccggtttta tgcagtggga gaacgcattc ttcccgtgga ccctgaacta
    54661 cgacgaaatc gacatggtgc tggaagggga gctccatgtt cgccacgaag gcgaaaccat
    54721 gatcgctaaa gcaggcgacg tgatgtttat cccgaaaggc tccagcatcg aatttggtac
    54781 gacatccagc gtgaaattcc tgtatgtcgc ctggccggct aactggcaat ccctatgaaa
    54841 gatttcatca ccgaagcatg gctaagagcg aaccatacgc tcagcgaagg cgcagagatc
    54901 catctccctg cggacagtcg cctgacgccc tctgcccggg agttgctgga aagccgccat
    54961 ctgcgcatca agtttattga cgagcagggc cgcctgtttg ttgacgatga acagcagcag
    55021 ccgcagcccg ttcatgggct aaccagtagc gatgaacatc cgcaggcgtg ctgcgaactg
    55081 tgccgccagc cggtggcaaa aaagccggat acgctgactc acctgtcggc ggaaaaaatg
    55141 gtcgccaaaa gcgatccgcg tctgggtttt cgcgcagttc tcgacagcac cattgcgctg
    55201 gcagtgtggc tgcaaattga actggcggaa ccgctgggct gccaggcgat tgtcgggctt
    55261 tctgacgaag atctgcaccg gctttctcat cagccgttgc gttatcttgg tcacgatcat
    55321 ctggtgccgg aagcaagcca cgggcgcgat gcggcgttgc tgaatctgct acgcaccaaa
    55381 gtgcgggaag ccgaaaccgt agcggcgcag gtgtttatca cccgcagttt tgaagtatta
    55441 cgcccggaca tcttgcaggc gctgaaccgc ctttccagca cggtctacgt gatgatgatc
    55501 ctgagcgtaa cgaagcagcc gctaacggtg aagcagatcc aacaacgact gggggaaacg
    55561 caatgattat tgaacgttgt tgtgaactgg cgttgcgagc gcccgccaga gtggtttttc
    55621 cggatgcgtt agatcaacgt gtgctgaaag ctgcgcaata tttacatcaa caaggtctgg
    55681 caacgcccat tctggtcgcc aatccgtttg aacttcgtca gtttgcgctc agtcacggcg
    55741 tggcgatgga cgggctacag gtgatagatc cgcatggcaa cctcgcaatg cgggaagaat
    55801 ttgctcatcg ctggctggcc cgcgcgggcg aaaaaacgcc gccggatgcg ctggaaaaac
    55861 ttaccgatcc gctgatgttc gccgccgcaa tggtcagcgc cggtaaagcg gatgtctgta
    55921 tcgcgggcaa cctctcttcc acggcgaatg tgctgcgtgc cggattacgc attatcggct
    55981 tgcagccagg ctgtaaaacg ctctcatcca ttttcctgat gctgccacag tacagcggtc
    56041 cggcgttggg ctttgccgat tgcagcgtgg taccacagcc gacggcggcg cagctggcgg
    56101 atatcgcgct tgccagcgcc gaaacctggc gcgccatcac cggagaagag ccgcgcgtgg
    56161 cgatgctgtc gttttccagt aacggtagcg cccgtcaccc ctgcgttgcc aatgtccagc
    56221 aggcgacaga aatcgtccgt gagcgcgcac caaagctggt agtcgatggc gagttgcagt
    56281 ttgacgccgc cttcgtgccg gaagtggcgg cgcaaaaagc gcctgccagc ccgctacagg
    56341 gcaaggccaa tgtgatggtt tttccgtcgc tggaagccgg aaatattggc tacaaaatcg
    56401 cacaacgact cggcggatat cgtgccgtcg ggccattgat acaaggactt gccgcgccga
    56461 tgcacgatct ctctcgtggt tgtagtgtgc aggaaattat cgagctggcg ctggtggcag
    56521 ctgtgccgcg tcagacagaa gtgaaccgcg aaagcagttt acaaacactg gttgaatgaa
    56581 cggtcccgtt ctggaccctg ataaagagga aagcacaatg gaagcattag gaatgatcga
    56641 aacccggggc ctggttgcac tgattgaggc ttctgacgcg atggtgaaag ctgcgcgcgt
    56701 caagctggtt ggtgttaagc agattggcgg cggcctgtgt actgccatgg ttcgtggtga
    56761 tgttgcagcc tgtaaagcgg cgaccgatgc cggtgccgca gcggcacagc gtatcggcga
    56821 gctggtatct gtacacgtta tcccgcgtcc gcacggcgac ctggaagaag tgttcccgat
    56881 cggcctgaaa ggcgatagca gcaacctgta atcaccctgt gtgacgtcga ttcgggcgtc
    56941 acgctctctc atttttcatt ttaccttccg cggagggtag ggggagcttt tacctgaaat
    57001 acgccacgga ggcgggtatg aaactggcag tcgtcactgg acaaattgtt tgtaccgtac
    57061 gccatcacgg actggcgcat gacaaattgc tgatggtgga aatgattgat ccacatggta
    57121 atcctgacgg gcaatgtgcc gtcgccatcg acaatattgg cgcgggaacc ggggagtggg
    57181 tgttgctggt gagtggcagt tccgcccgcc aggcgcataa aagcgaaacg tcaccggtcg
    57241 atctgtgcgt gattggcatc gtcgatgagg tggtgtctgg cggtcaggta attttccaca
    57301 aataaggcag aacatcatga atcaacagga tattgaacag gtggtgaaag cggtactgct
    57361 gaaaatgcaa agcagtgaca cgccgcccgc cgccgttcat gagatgggcg ttttcgcgtc
    57421 cctggatgac gctgttgcgg cagccaaaat tgcccagcaa gggttaaaga gcgtggcaat
    57481 gcgccagtta gccattgctg ccattcgtga agcaggcgaa aaacacgcca gagatttagc
    57541 ggaacttgcc gtcagtgaaa ccggcatggg tcgcgttgaa gataaatttg ccaaaaacgt
    57601 cgctcaggca cgcggcacgc caggcgttga gtgtctctct ccgcaagtgc tgacgggcga
    57661 caacggcctg actttaattg aaaacgcgcc ctggggcgtg gtggcttcgg tgacgccgtc
    57721 cactaacccg gcggcaaccg taattaataa cgccatcagc ctgattgccg cgggcaacag
    57781 cgtcattttt gccccgcatc cggcggcgaa aaaagtctcc cagcgggcga tcacgctgct
    57841 caaccaggcg attgttgccg caggcgggcc ggaaaactta ctggttactg tggcaaatcc
    57901 ggatatcgaa accgcgcaac gcttgttcaa gtttccgggt atcggcctgc tggtggtgac
    57961 cggcggcgaa gcggtagtag aagcggcgcg taaacacacc aataaacgtc tgattgccgc
    58021 aggcgctggc aacccgccgg tagtggtgga tgaaaccgct gacctcgccc gtgctgctca
    58081 gtccatcgtc aaaggcgcgt ctttcgataa caacatcatt tgtgccgacg aaaaggtact
    58141 gattgttgtt gatagcgtag ccgatgaact gatgcgtctg atggaaggcc agcacgcggt
    58201 gaaactgacc gcagaacagg cgcagcagct gcaaccggtg ttgctgaaaa atatcgacga
    58261 gcgcggaaaa ggcaccgtca gccgtgactg ggttggtcgc gacgcaggca aaatcgcggc
    58321 ggcaatcggc cttaaagttc cgcaagaaac gcgcctgctg tttgtggaaa ccaccgcaga
    58381 acatccgttt gccgtgactg aactgatgat gccggtgttg cccgtcgtgc gcgtcgccaa
    58441 cgtggcggat gccattgcgc tagcggtgaa actggaaggc ggttgccacc acacggcggc
    58501 aatgcactcg cgcaacatcg aaaacatgaa ccagatggcg aatgctattg ataccagcat
    58561 tttcgttaag aacggaccgt gcattgccgg gctggggctg ggcggggaag gctggaccac
    58621 catgaccatc accacgccaa ccggtgaagg ggtgaccagc gcgcgtacgt ttgtccgtct
    58681 gcgtcgctgt gtattagtcg atgcgtttcg cattgtttaa ggagtaacag atggcgcacg
    58741 acgaacaatg gctcacccca cgtctgcaaa cggcggcaac gctgtgtaac cagacgcccg
    58801 ccgcgacgga atctccgctg tggctgggag tggatctggg cacctgcgat gtggtgtcga
    58861 tggttgtcga ccgcgacggt cagccagtgg cggtatgcct cgactgggcc gatgtggttc
    58921 gcgacggcat cgtctgggat ttcttcggcg cggtcaccat tgttcgtcgc catctcgaca
    58981 cgctcgagca gcaattcggt cgtcgtttta gccatgcggc gacttcattt cctccaggta
    59041 cggacccgcg tatttccatc aacgtgctgg agtctgccgg gctggaagtg agccatgtgc
    59101 tggatgagcc aaccgcagtc gcggatctgc tgcaactgga caacgccggt gtagtggata
    59161 tcggcggtgg cactaccggc attgccatcg tgaaaaaggg caaagtgacg tactcggcgg
    59221 atgaagccac cggcggacat cacatctctc tgactttcgc cggaaatcgc cgtatttcgc
    59281 tggaagaggc ggagcagtac aagcgcggtc acggtgaaga gatttggcct acggtgaaac
    59341 cggtgtacga aaaaatggcg gacattgtcg cccggcatat tgaagggcag ggcattactg
    59401 atttatggct ggcgggcggc tcctgtatgc aaccgggcgt ggcagaactg tttcgcaaac
    59461 aattcccggc gttacaggtg catttaccgc agcacagttt gtttatgacc ccgctggcga
    59521 tcgccagtag tgggcgagag aaagcggagg ggctatatgc aaaatgaatt gcagaccgcg
    59581 ctctttcagg cgttcgatac cctgaatctg caacgggtaa aaacatttag cgttccaccg
    59641 gtgacgcttt gcggtccggg ctcggtgagc agttgcggac agcaagcgca aacgcgtggg
    59701 ctgaaacatc tgttcgtgat ggcagacagc tttttgcatc aggcagggat gaccgccggg
    59761 ctgacgcgta gcctggccgt taaaggtatc gccatgacgc tctggccatg tccggtgggc
    59821 gaaccgtgca ttaccgacgt gtgtgcagcc gtggcgcagt tgcgtgagtc aggttgtgac
    59881 ggggtgatcg catttggcgg cggctcggtg ctggatgcgg cgaaagccgt ggcgttgctg
    59941 gtgacgaacc cggatagcac gctggcagag atgtcagaaa ccagcgttct gcaaccgcgc
    60001 ttgccgctga ttgccattcc aactaccgcc ggaaccggct ctgaaaccac caatgtaacg
    60061 gtgattatcg acgcggtgag cgggcgcaag caggtgttag cccatgcctc gctgatgccg
    60121 gatgtggcga tcctcgacgc cgcattgacc gaaggtgtgc cgtcgcatgt cacggcgatg
    60181 accggcattg atgcgttaac ccatgccatt gaagcataca gcgccctgaa cgctacaccg
    60241 tttaccgaca gtctggcgat tggtgccatt gcgatgattg gcaaatcgct gccgaaagcg
    60301 gtgggctacg gtcacgacct tgccgcgcgc gagagcatgt tgctggcttc atgtatggcg
    60361 ggaatggcgt tttccagtgc gggtcttggg ttgtgccacg cgatggcgca tcagccgggc
    60421 gcggcgctgc atattccgca cggtctcgcg aacgctatgt tgctgccaac ggtgatggag
    60481 tttaaccgga tggtttgtcg cgaacgcttt agtcagattg gtcgggcact gcgaactaaa
    60541 aaatccgacg atcgtgacgc tattaacgcg gtaagtgagc tgattgcgga agttgggatt
    60601 ggtaaacgac tgggcgatgt tggcgcgaca tctgcgcatt acggtgcatg ggcgcaggcc
    60661 gcgctggaag atatttgtct gcgcagcaac ccgcgtaccg ccagcctgga acagcttgtc
    60721 ggcctgtacg cagcggcgca ataaatgccg cgtcttatcc ggtctgtggg attgcacatg
    60781 tagtgcggat aaggcttcac gcagcatccg ctaataaata acaactaaca gggagtaaag
    60841 gcgatgggaa ttaacgaaat catcatgtac atcatgatgt tctttatgct gatagctgcc
    60901 gttgacagga tcctgtcaca gttcggcggt tctgctcgtt tcctcggtaa gttcggtaaa
    60961 agtatcgaag gatccggcgg tcagttcgaa gaaggcttta tggcaatggg cgcactgggc
    61021 ctggcgatgg tcggtatgac cgcgctggca ccggtactgg ctcacgtact cgggccggta
    61081 attattccgg tttacgaaat gctcggcgct aacccatcga tgttcgccgg aacactgctg
    61141 gcgtgcgata tgggcggctt cttcctcgcc aaagagctgg cgggcggcga cgtagccgcg
    61201 tggctatact ctgggttaat tctcgggtcg atgatggggc caacgattgt gttttccatt
    61261 ccggtggcgc tcggcattat cgaaccttct gaccgtcgtt atctggcgct cggcgtgctg
    61321 gcgggcattg tgaccattcc gattggttgt atcgctggtg gtctggttgc tatgtactcc
    61381 ggtgttcaga tcaacggcca gccggtggaa ttcactttcg ccctgatcct gatgaacatg
    61441 atcccggtga tcattgttgc gattctggtg gcgctggggc tgaaattcat cccagaaaaa
    61501 atgatcaacg gcttccagat cttcgccaaa ttcctcgttg cattgatcac cctcggtctt
    61561 gccgctgcgg tagtgaaatt cctgcttggc tgggaactga tccccggtct ggatcctatc
    61621 tttatggccc ctggcgataa acccggtgag gtgatgcgcg ccattgaagt tatcggttct
    61681 atctcctgcg ttctgttagg ggcgtatccg atggtgctgc tgctgacccg ctggtttgaa
    61741 aaaccgctga tgagcgtcgg taaagtgctg aatatgaaca acatcgcggc agccggcatg
    61801 gtggcaacgc ttgccaacaa catcccgatg ttcggcatga tgaagcagat ggatacccgc
    61861 ggcaaagtca tcaactgcgc cttcgccgtt tccgctgctt tcgccctggg cgaccactta
    61921 ggcttcgccg ctgccaacat gaatgccatg atcttcccga tgattgtcgg caagttgatc
    61981 ggcggcgtaa cggcgattgg cgtggcgatg atgctggtgc caaaagacga cgcgaccgcg
    62041 gctaaaaccg aagcggaggc acaatcgtga acactcgcca gctactgagc gtcggtatcg
    62101 atatcggcac caccaccacc caggtgattt tctcccacct ggaactggtt aaccgtgcgg
    62161 cggtgtcgca ggtgccgcgc tacgaattca tcaaacgcga aattagctgg caaagcccgg
    62221 tgttctttac ccctgtcgat aaacagggtg gtttaaaaga agcggaactg aaatcgctga
    62281 ttttagagca atatcaggct gcgggcattg cgccggaaag cgttgattct ggtgccatca
    62341 tcatcaccgg tgaaagcgcg aaaacccgca atgctcgccc ggcggtgatg gcgctctctc
    62401 agtcgctggg cgattttgtc gtcgccagcg ccgggccgca cctcgaatcc gtgatcgccg
    62461 gtcacggtgc cggggcgcaa accctttctg aacaacggct gtgtcgggta ctgaatatcg
    62521 acatcggcgg tggcaccgcg aactacgccc tgttcgatgc cggaaaaatc agcggcactg
    62581 cctgcctcaa cgtcggtggt cgcctgctgg aaaccgacag ccaggggcgc gtggtttacg
    62641 ctcataaacc ggggcagatg attgtggatg agtgtttcgg tgcaggcact gacgtccgtt
    62701 cgctgaccgg cgcgcagctg gtgcaggtta cccgacggat ggcagagctg attgtcgaag
    62761 tgattgacgg aacgctttcg ccgctcgcgc aggcattaat gcaaaccggt ttgctgcccg
    62821 caggtgttac gcccgaaatc attacgcttt ctggcggcgt gggcgaatgt tatcgccacc
    62881 agcccgctga cccgttctgt tttgccgata ttggcccgct actggcgacg gcgctacatg
    62941 accatccgcg cctgcgtgag atgaacgtgc agtttccggc gcaaaccgtg cgcgccacgg
    63001 tgattggcgc gggcgcacat accctttcgc tctctggcag cacaatctgg ctggagggcg
    63061 tacaactgcc gctgcgcaat ttgccggtgg cgatcccgat tgatgaaacg gatctggtga
    63121 gtgcctggca acaggcgctg attcagctgg atcttgatcc caaaactgac gcgtacgtgc
    63181 tggcgcttcc cgcctcgctg cctgtgcgtt acgccgcggt actgacggtc atcaacgcgt
    63241 tggtcgattt cgtcgcgcgt tttccgaatc cgcatcccct gctggtggtg gccgggcagg
    63301 actttggtaa agctctgggc atgctgttgc gcccacagct acaacaactc ccgttggcag
    63361 tcattgacga agtgattgtc cgcgcggggg actatatcga cattggtacg cctctttttg
    63421 gcggatcggt tgtgccggtg acggtgaaat cactcgcatt tccttcctga gggaacgact
    63481 tatgaaacta aagaccacat tgttcggcaa tgtatatcag tttaaggatg taaaagaggt
    63541 gctggctaaa gccaacgaac tgcgttcggg ggatgtgctg gcgggcgtcg cagcggcaag
    63601 ctcacaggag cgcgtggcgg caaagcaggt gttgtcggaa atgaccgtag cggacatccg
    63661 caataatccg gtgattgcct atgaagatga ctgcgtgacg cggctgattc aggacgatgt
    63721 taacgaaacg gcctacaacc agattaaaaa ctggagcatc agcgaactgc gtgagtatgt
    63781 gctgagcgat gaaaccagcg tggacgacat tgcctttacc cgcaaagggc tgacctcgga
    63841 agtggtcgcg gcggtagcga agatttgctc caacgcggac ctgatctacg gcgcgaagaa
    63901 aatgccggta atcaaaaagg ccaataccac catcggtatt ccgggcacct tcagcgcccg
    63961 tttgcagcca aatgacaccc gtgacgacgt gcaaagtatc gccgcgcaaa tctacgaagg
    64021 gctttccttc ggggtgggcg atgcggtgat cggcgttaac ccggtgactg acgacgtgga
    64081 aaacttaagc cgcgtgctgg acaccatcta tggcgtgatc gacaaattca acatcccaac
    64141 tcagggctgc gtactggcgc acgtcaccac ccagatcgaa gcgattcgtc gcggcgcgcc
    64201 tggcggactg attttccaga gtatctgtgg cagcgaaaaa gggctgaaag agtttggcgt
    64261 ggaactggcg atgctcgacg aagcgcgcgc agtgggtgca gagttcaacc gtatcgccgg
    64321 ggaaaactgc ctctacttcg aaaccggaca aggttcagcg ctatccgctg gcgctaactt
    64381 cggcgcagac caggtgacga tggaagcacg taactatggg ctggcgcgtc attacgatcc
    64441 gtttatcgtc aacaccgtgg tcggctttat tgggccggag tatctctaca acgaccgcca
    64501 gattatccgt gcgggcttag aagatcactt tatgggcaag ctgagcggca tctctatggg
    64561 ctgtgactgc tgttacacca accacgctga cgctgaccag aacctcaacg aaaacctgat
    64621 gatcctgctc gccaccgcag gctgcaacta catcatgggg atgccgctgg gtgatgacat
    64681 catgctcaac taccagacca ccgcattcca cgataccgcc actgtgcgtc agttactcaa
    64741 cctgcgcccg tcaccggagt ttgaacgctg gctggaaagc atgggcatta tggcaaacgg
    64801 tcgcctgacc aaacgggcgg gcgatccgtc actgttcttc tgatgacgcg gagataacac
    64861 atcatggatc aaaaacagat tgaagaaatt gtacgcagcg tgatggcgtc aatgggacaa
    64921 acggcccccg cgccgtcaga agcaaagtgc gccactacca cctgtgcggc accggtgacc
    64981 tcggaaagct gcgcgctgga tttaggttct gcggaagcaa aagtgtggat tggtgttgaa
    65041 aacccacatc gtgcggatgt gttaacagaa ctgcgtcgca gcaccgtggc ccgcgtttgt
    65101 accggtcgtg ccggtccgcg tccgcgtacg ctggcgctgc tgcgtttcct ggccgatcac
    65161 tctcgttcga aagataccgt actgaaagaa gtgccggaag agtgggtgaa agcgcagggc
    65221 ttgctggaag tacgctccga aattagcgac aaaaatctct acctgactcg cccggatatg
    65281 ggccgccgcc tgtgtgcaga agctgttgaa gcgctgaaag cacagtgtgt tgctaatccg
    65341 gacgtacagg tcgttatttc tgatggcctg tcaactgatg ccatcactgt gaactacgaa
    65401 gagatcctgc cgccgctgat ggcgggcctg aaacaggccg ggctgaaagt cggcacgcca
    65461 ttctttgttc gttatggtcg cgtgaagatt gaagatcaga tcggtgagat cctcggcgcg
    65521 aaagtagtga tcctgctggt gggcgaacgt ccggggttgg gtcagtcaga aagcctctcc
    65581 tgctacgccg tttactcgcc gcgtatggcg accaccgttg aggccgatcg cacctgtatc
    65641 tctaacattc accagggcgg cacgccgccg gttgaagccg cagccgtcat tgtggatttg
    65701 gcgaaacgta tgctggagca gaaagcatcc ggcatcaaca tgacccgata aggaggcatc
    65761 atgccagctt tagatttgat tcgaccgtcg gtaaccgcca tgcgggtgat tgcctccgtt
    65821 aacgccgatt ttgcgcgtga actgaaattg ccgccacata ttcgtagcct cggtctgatt
    65881 tctgccgatt ctgacgacgt aacctacatt gcggctgacg aagcgaccaa acaagcgatg
    65941 gtagaagtgg tatatggtcg ctcgctgtat gcgggcgcgg cacacggccc gtcaccgact
    66001 gccggtgagg tgctgattat gcttggtggg ccaaacccgg cggaagtgcg tgccggtctg
    66061 gatgcgatgg ttgcgcatat tgaaaatggc gctgctttcc agtgggctaa tgatgcgcaa
    66121 gatacggcat tcctggcaca tgtagtttcg cgtaccggtt cttatctctc atcaaccgcc
    66181 gggatcacgc ttggcgatcc gatggcgtat ctggtggcac cgccgctgga agcgacctac
    66241 ggcattgatg cagcgttgaa atctgccgac gtacagctgg tgacctatgt cccgccaccg
    66301 tctgaaacta actactcggc agcattttta accggtagcc aggcagcgtg taaagcggcc
    66361 tgtaacgcct ttaccgatgc agtgctggaa atcgcgcgta atccaatcca gcgtgcgtaa
    66421 cggaggttgc caatgatcaa tgcactggga ttgctggaag tggacggcat ggtcgccgcg
    66481 atagatgcag cggatgccat gctcaaagca gctaacgttc gtctgctcag tcacgaagtg
    66541 cttgacccag gtcgcttaac gctggtggtg gaaggcgatc tggcggcgtg tcgtgctgcg
    66601 ctggacgcag gttgtgctgc cgcgatgcgt accgggcgtg tcatcagccg taaggagatc
    66661 ggtcggccag acgatgacac ccagtggctg gttactggct ttaaccgcca gccgaagcaa
    66721 cccgtaaagg aacccgacgc gccagttatc gtcgcggaat ctgctgacga gttgttggcg
    66781 ctgttaacat cagtacgtca gggagtgacg gcaggagaag tggctgccca ctttggctgg
    66841 ccgctggaaa aagccagaaa tgcgctcgaa cagctctttt ctgctgggac gttacgtaaa
    66901 cgcagtagtc gttatcgtct caagccccat taacctgtcg gaggtgccgg gtgtcctaca
    66961 acacccggca ttaacatcat gaaaaagacc cgtacagcca atttgcacca tctttatcat
    67021 gaacccttac ccgaaaacct gaagctcacg ccgaaggtcg aagtggataa tgttcatcaa
    67081 cgacagacaa cggatgtcta tgaacatgct ttaacgatta ccgcctggca gcagatttac
    67141 gatcagctgc atccgggcaa gtttcatggt gaatttacgg aaattctact cgatgatatt
    67201 caggtttttc gtgaatacac cggtctggcg ctgcgtcagt cgtgtctggt ctggccgaac
    67261 tcgttctggt ttggcattcc ggcgacgcgc ggtgagcagg gatttatcgg ttcgcaatgt
    67321 ctgggaagcg cagaaatagc gacgcgccct ggtggaaccg agtttgaatt aagtacgccg
    67381 gacgattaca cgatcctggg cgtagtgctt tctgaagatg tcatcacccg tcaggctaac
    67441 tttttgcata atccggatcg ggtattacat atgttgcgta gccagtcggc gctggaagtg
    67501 aaagagcagc ataaagccgc gctgtggggc tttgtccaac aggcgctggc gacgttttgc
    67561 gagaacccgg aaaatctcca tcagcctgca gtgcgaaaag tgctggggga taatttgcta
    67621 atggcgatgg gggcgatgct ggaagaagcg cagccaatgg tgacggcgga aagcatcagt
    67681 catcagagtt accgtcggtt gctttcccgc gcccgtgaat atgtgctgga aaacatgtcc
    67741 gaaccggtga cggtgctgga cttgtgtaat caactgcatg tcagccgccg cacgctacaa
    67801 aacgcgtttc acgctatttt aggaattggc ccgaacgcgt ggctgaaacg cattcgcctg
    67861 aacgccgtac gccgcgaact gataagcccg tggtcgcaaa gcacaacggt aaaagacgcc
    67921 gccatgcagt ggggattctg gcatctgggg caatttgcca cggattacca gcagctgttt
    67981 gccgagaagc cgtcgttgac gttgcatcag cggatgcggg aatgggggtg agggagttac
    68041 acccaatccc tgaccttaat aaactcactt aacgccgctt ctgggctgcc atcttttggc
    68101 tgataatcat actcccagcg taccagtggc ggcattgaca tcaggatcga ctcggtgcgc
    68161 ccgccagttt gcaggccaaa cagagtgccg cgatcccaga ccagattgaa ctcgacataa
    68221 cgaccgcgac ggtagagctg aaaattgcgc tcgcgctcgc cgtaggccat cgctttacgt
    68281 cgctctacaa ttggtaaata agcgtcggtg tagcctttgc ctaccgcctg cataaaggca
    68341 aaacagtggt cgaaatctgg cgtgtttaga tcatcaaaga acagcccgcc aataccgcgc
    68401 tgttcgttgc gatgtttgag gtagaagtaa tcgtcgcacc actttttgta acggggataa
    68461 acgtctttac caaatggcag gcacaggtca cgggcggtgc ggtgccagtg aatggcgtct
    68521 tcttcaaaac catagaaagg ggttaaatcg aagccgccgc caaaccacca gactggctct
    68581 gcacccggtt tttcggcaat aaaaaagcgc acattcgcgt ggctggtggg aacatacgga
    68641 ttatgcggat gcactaccag agaaacgccc atcgcctcga aactgcgccc ggcaagttcc
    68701 gggcgatgag cggtggcgga agcaggcatc gcctcgccgt ggacgtgaga aaagttgacg
    68761 cctgcctgtt cgaaaacacc accattacgc aacacccgac tacgcccgcc gccgccacct
    68821 tcgcgctgcc agctatcttc gacaaattct gcgccatcga cggcggtcag ctgctgacaa
    68881 atcgtatcct gaaggttgag cagaaactgt ttaacctggt gtgcgtcggg tttcataact
    68941 tatcgctttt tcgaatgtgc tttctggttg tcgaaccagt ggaaataact gatcacgcct
    69001 tcagcaatcg ctgtggcgat tttctgacga aacgccgccg tgcctaacag tcgctcttct
    69061 tccgggttgg tgataaacga ggtttccacc agcaccgaag gaaccgacgg cgatttcaac
    69121 accacaaacg cggcttgttc ggtgttgcgg ctgtgcagtt tatgcaccgg cttaatcttt
    69181 ttcagaatat gcgagccgag cgtcaggctg tttttaatag tatcggtttg caccagatca
    69241 aacagcactt gctgcaatag atgatccttg tcagtcgcct ttttaccggc aacttcatcg
    69301 gcgcggtttt cgcgttcaga caggtatttc gccattgcgc tacttgcccc acggttagag
    69361 agggcaaata ccgaagcacc ggcagctttc gggttggtaa agccatcggc atgaattgac
    69421 ataaacagat ctgcgccatg tttatgggcg atttcaacgc gatcgtaaag tgggataaac
    69481 gtatcgccag aacgcgttaa acgcgcatca atcccatgat tacgcaaaat ggaacggacg
    69541 tttttagcaa tcgccagcac cacatgtttt tctttcgaac cgttgcgccc gatcgctccg
    69601 gtatcaatcc cgccgtggcc tggatcgaga acaacgacac gtttgccgcc agatttttta
    69661 gctttcggct tgctgtgtcc gttgctggtt tttaaaggtt cgtctttggc gatggcttgc
    69721 gacattcctg acaacgtcag ggcagccaaa ccggctttca gcacctggcg gcgcgaagtg
    69781 agtgttttta gtggtttaaa agtgctcata cggcctgagt tgtaataata aagttccaga
    69841 tgttatatcg tatcgcgtaa tccgttacga ccgtttgctg ttgagaattg ttttactttt
    69901 catttcaata gttgacaact ccccattatg ccactttttc aaccgatttt cgtcagatat
    69961 tggctaattc gaccgttcac gccataatca gccttttaac ccgcaaaaag gtaacgaata
    70021 ccatggagat acgcgtattt cgccaggaag atttcgaaga ggtcatcacc ctttgggagc
    70081 gttgcgactt gctgcgtccg tggaacgatc cggaaatgga catcgagcgt aagatgaacc
    70141 atgacgtcag tttgtttctg gtcgctgagg tgaacggtga agtggtcgga acggtgatgg
    70201 gcggttatga cgggcatcgc gggtctgctt attatcttgg cgtgcatcca gagtttcgtg
    70261 ggcgtgggat tgccaatgcg ttgcttaacc ggctggagaa aaagctgatt gctcgcggct
    70321 gcccgaaaat tcagatcaac gtgccggaag ataacgacat ggtgctcgga atgtatgaac
    70381 gcctgggata tgaacacgcc gacgtgctga gtctgggtaa gcgtttgatt gaagatgaag
    70441 agtactgagt ttcatcctgt ccattatgat gcgcacggtc gcctgcgttt acccttgctc
    70501 ttctggcttg tgctattgct tcaggcgcga acctgggtgc tgtttgtcat tgccggtgcg
    70561 tcgcgcgagc agggtactgc gctgctaaat ctgttttatc ccgatcacga taatttctgg
    70621 ctggggctga ttcctggcat tccggcggtg ctggcgtttt tgttgagcgg tcggcgggct
    70681 acgtttcctc gcacctggcg tgtactctat tttctgttgc tgttggcgca ggtggtttta
    70741 ctttgctggc aaccgtggct gtggctgaac ggtgaatccg ttagcggtat cggtctggcg
    70801 ctggtagtgg cggatattgt ggcgttaatc tggctgctga ccaatcgacg tttacgcgct
    70861 tgttttaatg aagtaaaaga ataacggcac tttttggtga atttgcactc caagcaacgt
    70921 tattgaataa ccaaaggaag tgacatgaaa tcgctgcgtt taatgttatg cgctatgcca
    70981 ttgatgctga ccggctgttc cacgatgtcg tcagttaact ggtctgccgc taacccgtgg
    71041 aactggtttg ggtcgtccac caaagtgagc gagcagggcg ttggtgaatt aacggcgtcc
    71101 acaccactgc aagaacaagc cattgccgat gcggttgatg gcgattatcg cctgcgcagc
    71161 ggaatgaaaa ccgcgaacgg caacgtggtg cgcttttttg aagtgatgaa gggcgacaac
    71221 gtggcgatgg tgattaacgg cgatcaaggc acgatcagtc gcattgacgt gctggatagc
    71281 gatattcctg ctgacaccgg tgttaaaatc ggtacaccgt ttagcgacct ttacagcaaa
    71341 gcatttggca attgccaaaa agctgatggt gatgataatc gtgccgtcga atgtaaagcc
    71401 gaaggcagtc aacatattag ctaccagttc agcggggaat ggagtggtcc tgaagggtta
    71461 atgccttcgg acgataccct gaaaaactgg aaagtcagta aaattatctg gcggcgttaa
    71521 tttgcgtctg aacaaaccgc cgccgcgaaa aaacacgtaa aatagcgctc aacaatgcca
    71581 cggattgcgt ggcattatta ttttcaggag gaacaatgtc tcaggttcag agtggcattt
    71641 tgccagaaca ttgccgcgcg gcgatttgga tcgaagccaa cgtgaaaggg gaagttgacg
    71701 ccctgcgtgc ggccagtaaa acatttgccg acaaactggc aacttttgaa gcgaaattcc
    71761 cggacgcgca tcttggtgcg gtggttgcct ttggtaacaa catctggcgc gctctgagcg
    71821 gcggcgttgg ggcagaagag ctgaaagatt ttccgggcta cggtaaaggc cttgcgccga
    71881 ccacccagtt cgatgtgttg atccacattc tttctctgcg tcacgacgta aacttctctg
    71941 tcgcccaggc ggcgatggaa gcctttggtg actgcattga agtgaaagaa gagatccacg
    72001 gcttccgttg ggtggaagag cgtgacctga gcggctttgt tgacggcaca gaaaacccgg
    72061 cgggtgaaga gacgcgtcgc gaagtggcgg ttatcaaaga cggcgtggat gccggcggca
    72121 gctatgtgtt tgtgcagcgt tgggagcaca acctgaagca gctcaaccgg atgagcgttc
    72181 acgatcagga gatgatgatc gggcgcacca aagaggccaa cgaagagatt gacggcgacg
    72241 aacgtccgga aacctctcac ctgacccgcg ttgatctgaa agaagatggc aaagggctga
    72301 agattgttcg ccagagcctg ccgtacggca ccgccagtgg cactcacggt ctgtacttct
    72361 gcgcctactg cgcgcgtctg cataacattg agcagcaact gctgagcatg tttggcgata
    72421 ccgatggtaa gcgtgatgcg atgttgcgtt tcaccaaacc ggtaaccggc ggctattatt
    72481 tcgcaccgtc gctggacaag ttgatggcgc tgtaagcgtt tctgattcat tgattaaagg
    72541 ccaggcagta acattactga ctggcctttt tattactttt gctttaacgc cgcatacacc
    72601 tgatcaacta cccaaccata cgttgcaatc ggcagctgac cgctttcgaa catattggga
    72661 ttcttttgcg gatcggcaac cggcgaatgt ggcttgttgc ttaacatcac aatcgccata
    72721 tgattcaccg gatcgataac ggtcaccgtt ccggtccagc cagtgtgacc gtaggtttgc
    72781 gggcttgcca gcgtgccaaa cgtcggcgtc atggtggcat taccattcac gcgccagccg
    72841 aggccaaaag tggcatcttc cttagagctg gtggtgaaca tcttcaccgt ttccgcactg
    72901 aacagctgca catcgccata gcccccgccg ttcagcatcg tttgcattaa caccgcaata
    72961 tcgccggtat tggaaaacaa acctgcgtgc ccggaaacgc cgcccatcga ataaaaggct
    73021 ttttcatcgt gcacctgacc ccagtgagtg gaggtgcgga tattcgggaa gtggatcact
    73081 ccatcgcggg tattgccgtt caattccgtg gcggcaattt gttgcggttt aaagcctttc
    73141 agcagcgggt taaataccgt atgtgtcagg ccgagcgggc gataaatcga ctcttcaaca
    73201 tagcggtcaa gcggctgacc ggtgaccgac tcgacgataa atccaagcag catataatcg
    73261 acatcactgt aaatatgctt gctgccgggc tgatactcta gcggcgtgcg cttgatcatc
    73321 tccagcgtct ggcctttatc ctgggaatat aacgcgcccg cgaccgcttt attcggatat
    73381 tgtgggtccg ccgggaaacc gccgctgtga tgcagcaggt cagaaatccg cagcgtgttt
    73441 ttgcctttga tggtgtcatt tggactatcg gcaaaccccg aaatatattt cgcaatcaga
    73501 tcgtcaggat gcagtttgcc ttcgctcatt agcttttgca gagcgaagtt agtggcatac
    73561 attttggtgt ttgaggccag gtcatacagc gtcccggtgg tagcttttac aggctgcgcc
    73621 atcaatacgc tgccatcgta ctttttcgcc gctccccagg ctttgcgata cacaatctga
    73681 ttatctttta taatcagcag gtttacgccg ggataaccgg cgtccacttg ctggctaatc
    73741 cagcgatcca tctggttaag ccgttcgacg ttgaaccctg ctttctctgg cgaactttct
    73801 gtcagaacag ggtattttgc ggcgctgacg ctacaactaa cagccagcag agaaagatag
    73861 agcattgtcc gtttcatgat atcagtccag attgacgtta cggcagccaa aaagcgtggt
    73921 gaaaataaac ccgcaaaccc atgccaccag aatcccgcca gcgtagactg ccatcgccgg
    73981 aagaatgcct tgtgctgaag tcatcagcgg cagcgccacc agaccagacg gcccaaaggc
    74041 gctgtttaag cccatcggca gaccccacca ggctatcaat ccgataaaca aaccgcccgc
    74101 cgcgccgcct aaacaggcgg taataaacgg tttcatgcgg ggcagggtga cgccataaat
    74161 caacggttcg ccaacgccca gcaggccggg aataatcgcc ccgcgtacct gactgcgtaa
    74221 tgcactgtgc ggttgcgccc gccagtagag tgccagcgcc gcgcccacct ggcccgcgcc
    74281 tgccatcgaa aggatgggaa ataagctgtt gaatccttgg ctgtccatta acgcgaggta
    74341 aacaggaata aagccctgat gcacgccaaa taccacggcg atcaggaaca gcccggctaa
    74401 aaccgcacaa ccgagcggat tactgttcag gtgcataaac agccacgaca taccttcgaa
    74461 taaccagccg cccagcggca taatgatcag ataggccagc gtagcggtga tcagtagggt
    74521 gattaacgag gtcagcagca tgtcgagatc gtcgggcata aagcggcgca ccatgccttc
    74581 gatgcgggcg caggcccacg cggcaatcaa cacgccgata atattgccgc gcggatcgat
    74641 gggcagacca aagaaatcgt gaaaaccggc gtagtaaccg gtggttgcag cggggttata
    74701 accgagcaaa aagagtgcgg caataattgc gccattgacg cccgtgccgc caaatgcctg
    74761 ggcggcgtta tagcccacca gaatcaccag gaaggtgaac aaacctttgc tgaacacctt
    74821 cataaaattc agcgcgtcgg gtagcgtgcc ctgagcatct gccggaacgt gcatcaccgt
    74881 ggcaattaac gtcgctatcc ccagcagcag accggcggca ataaaaccgg ggataagcgg
    74941 cgtgaagatg gtggcgaatt tggcgagaaa ttgttgcacg ccggaggttt gtttggcttt
    75001 taactgacgt ttgttctggg cggcgatttc tgcggcatcc tgtaccggtg cttctcccag
    75061 cagctcgctc atcgcttttg cggcacgatg cgctttacca ggcccaaaaa cgacctgcac
    75121 ctggtcactg gtcaaaatga cgcccttcac gccttccagc gttttgatat cgggatcaac
    75181 cagtgaactg tcatgtacac ccagacgcag gcgcgtcata cagttaccac aactggcgat
    75241 atttcccggt ccgccgacac gggtaagaat ggtgttcaga agttcactgc tgatctcttt
    75301 ggccatgggt tattccttgt ctaaaacctg acgaataaag ccgccgtgtt gatccaggcg
    75361 ttttttagct tctgcggcat cgagattttt cagcaccatc acaatggccg ttttacagtt
    75421 gcgctcgcag gcaattaacg ctgcttctgc ttgttcggca ttacatccgg tggcgttttt
    75481 aacgatattg acctggcgca catgcagttt ttcgttggtg gcgaccacat cgaccatcag
    75541 gttgccgaac actttgccgg atttaatcat cagcccggtg gaaagcatat tgagcaccag
    75601 tttctgcgcc gtgcctgctt tcatccgcga agaaccggtg accacttcag ccccaaccac
    75661 cggcgtaata gcaaactcag cggtggtgga aacggcgctc cccggattac aggaaattcc
    75721 cactgtacgg cagccgagct ggcgtgcata ttccagtccg gcaatcacat acggcgtgcg
    75781 accgctggca gcaatgccaa ccaccacatc ctgcgcggtt aatccgatgt ttttcaaatc
    75841 attaacaccg ccttcccggc tatcttcagc gccttccacc gcgtgctgaa tggcatattc
    75901 gccgccagca attaatccaa ctaccagacc cggtttcacg ccgtaggtgg gcggacattc
    75961 gctggcatcc agaatcccca gacgaccgga tgttcccgca ccgagataaa tcagacgacc
    76021 gccgccgctt acctgggcgt ggataacatc gattgccgcg gcgatatccg gcagtacgcg
    76081 ctcaacggca agcggtacgg ttttatcttc atcgttgata atccggcaca tttccagcgt
    76141 tgatacgcgg tcaatttcag ccgaggcggc gttcgagcct tcggtaatca tcttttcaag
    76201 ttgcatcggt ggtgacctta ctatttacga tcaaataatt gcagatgtat ggaatcatat
    76261 atcccgaatt gacagactta aaggaatcat tgagtctaca ctcgcagagt gtgaagagcg
    76321 tcataagaac ttaagcggaa tttttgctgc cagcccagga gagtggaaga tgttgtacct
    76381 gacgaaaata cgcaatgcgg aaagtgaatt taccgagaac gagcaaaaaa ttgctgactt
    76441 tctgcgggcc aacgtgagtg agctgaaatc cgtctcttcg cgcaagatgg cgaaacagct
    76501 cggcatcagc cagtcgagta ttgtgaagtt tgcccagaag ctcggcgcac aagggtttac
    76561 tgaattgcgt atggcgttaa taggcgaata cagtgccagc cgggagaaaa cgaatgccac
    76621 ggcgcagcac ctgcacagtt cgattaccag tgatgattcg ctggaagtga ttgcccgcaa
    76681 attgaatcgc gaaaaagagc tggcgctgga gcaaacctgt gcgttgttcg actacgcgcg
    76741 gctgcaaaaa atcattgagg tcatcagtaa agcaccgttt atccagataa cgggcctggg
    76801 cggatcggcg ctggtggggt gcgatctgtc gttcaaactg atgaaaattg gatatcgcgt
    76861 ggcctgcgaa gccgacacgc acgtacaagc caccgtttcc caggcgttga aaaaaggcga
    76921 tgtacaaatc gctatctctt acagcggcag taaaaaagag attgtcttgt gcgcagaagc
    76981 ggcacgaaag cagggggcaa cggtgattgc cattacttct ctggctgact ctccgctgcg
    77041 gcggctggcg catttcacgc tcgataccgt ttccggtgaa accgaatggc gtagctcgtc
    77101 gatgtctacc cgcacggcgc aaaattctgt taccgattta ctgtttgttg gtcttgtgca
    77161 actcaatgat gtggagtcat tgaaaatgat tcagcgcagc agtgagctaa cacaacgcct
    77221 gaaatgatgc ataaagcagc gactggattg agattttcct gaattagtga gctgatccgc
    77281 agtaatattt tgtttatcct gtattttcag agggaatgga gtgtaacgct ctgtattaac
    77341 aaggagagca ttaaaatggg taaactcacg ggcaagacag cactgattac gggcgcattg
    77401 cagggaattg gcgaaggaat tgccagaact tttgcgcgtc atggcgcaaa cctaatcttg
    77461 ctggatatct cccctgagat cgaaaagctg gcggacgaac tgtgtggtcg tggtcatcgc
    77521 tgtacggcgg ttgtcgccga tgtgcgtgac ccggcgtcgg tagccgcagc tatcaaacgc
    77581 gcgaaggaaa aagaagggcg cattgatatc ctggtgaata acgcaggcgt ttgtcgtctg
    77641 ggcagtttcc tcgatatgag cgatgacgat cgcgatttcc atattgacat caatattaaa
    77701 ggcgtatgga acgtcacgaa ggcggtgctg ccggagatga ttgcccgcaa agatggtcgc
    77761 attgtgatga tgtcttcagt cactggtgat atggtggccg atccgggtga aacggcgtac
    77821 gccttaacga aagcggcgat tgttggcctg acaaaatcgc tggcggtgga gtatgcgcag
    77881 tctggtattc gcgttaacgc catatgccct ggatacgttc gcacaccaat ggcggaaagc
    77941 attgcccgcc agtcgaaccc ggaagatcca gagtcggtgc tgactgaaat ggcaaaagca
    78001 atcccgctgc gtcgcctcgc cgatccgctg gaagtcggcg aactggcggc cttcctcgca
    78061 tcggatgaat ccagctattt aaccggtaca cagaatgtga ttgatggcgg cagcacactg
    78121 ccggagacgg ttagcgtcgg tatctgattc acctctgttt cctccctgca tttgtgggga
    78181 ggatttcgtc ttattccctt ttcaacttcc aaatcaccaa acggtatata aaaccgttac
    78241 tcctttcacg tccgttataa atatgatggc tattagaaag tcattaaatt tataagggtg
    78301 cgcaatggcc gttaacttac tgaaaaagaa ctcactcgcg ctggtcgctt ctctgctgct
    78361 ggcgggccat gtacaggcaa cggaactgct gaacagttct tatgacgtct cccgcgagct
    78421 gtttgccgcc ctgaacccgc cgtttgaaca gcaatgggcc aaagataacg gcggcgacaa
    78481 actgacgata aaacaatctc atgccgggtc atcaaaacag gcgctggcaa ttttgcaggg
    78541 cttaaaagcc gacgttgtca cttataacca ggtgaccgac gtacaaattc tgcacgataa
    78601 aggcaagctt atcccggcgg actggcagtc gcgcctgccg aataacagct cgccgttcta
    78661 ctccaccatg ggcttcctgg tccgtaaggg caacccgaag aatatccacg actggaacga
    78721 cctggtgcgc tccgacgtga agctgatttt cccgaacccg aaaacgtcgg gtaacgcgcg
    78781 ttatacctat ctggcggcat ggggcgcagc ggataaagct gacggtggcg acaaagccaa
    78841 aaccgaacag tttatgactc agttcctgaa aaacgttgaa gtgttcgata ccggcggtcg
    78901 tggcgcgacc accacctttg ccgagcgcgg cctgggcgat gtactgatta gctttgaatc
    78961 ggaagtgaac aacatccgca aacagtacga agcgcagggc tttgaagtgg tgattccgaa
    79021 aaccaacatt ctggcggaat tcccggtggc gtgggtcgat aaaaacgtgc aagccaacgg
    79081 taccgaaaaa gcagcaaaag cctatctgaa ctggctttac agcccgcagg cgcaaaccat
    79141 catcaccgac tattactacc gcgtgaataa cccggaagtc atggacaaac tgaaagataa
    79201 attcccgcag accgaactgt tccgcgtgga agacaaattt ggctcctggc cggaagtgat
    79261 gaaaacccac tttaccagcg gcggcgagtt agacaagctg ttagcggcgg ggcgtaatta
    79321 atgtttgcag tttcatcaag acgcgtcctg ccgggcttta ccttaagcct cggcaccagt
    79381 ctgctgtttg tgtgcctgat attgctgctg ccactctccg cgctggtgat gcagctttct
    79441 gagatgagct gggcgcagta ctgggaagtg atcaccaacc cgcaggtggt cgcggcctac
    79501 aaagtgacgc tgctgtcggc gtttgtggca tcaattttta acggcgtttt cggcctgctg
    79561 atggcgtgga ttttaacccg ttatcgcttt ccgggccgca cgctgcttga tgcgctgatg
    79621 gatttaccct ttgcgctgcc aacggcagtc gccggtttaa cgctggcctc gctcttttct
    79681 gtaaacggtt tttacggtga atggctggcg aagtttgata tcaaagtcac ctatacctgg
    79741 ctggggattg cggtggcaat ggcctttacc agcattccgt ttgtggtacg taccgtgcag
    79801 ccggtgctgg aagagttagg cccggaatat gaagaagcgg cagaaacgct tggcgcaacg
    79861 cgctggcaga gtttctgcaa agtggtgctg ccggaacttt ctccggcgct ggtggcgggc
    79921 gtggcgttgt cgtttacccg tagtcttggt gagtttggcg cggtgatatt tatcgccggg
    79981 aacatcgctt ggaagacgga agtgacgtcg ctgatgattt tcgttcgctt acaggagttt
    80041 gattacccgg cggcgagcgc gattgcttcg gtgatcctcg cggcatctct gctgctgctg
    80101 ttctcaatta acactctgca aagtcgcttt ggtcggcgtg tggtaggtca ttaatggcgg
    80161 aagtgacaca attgaagcgt tatgacgcgc gcccgattaa ctggggcaaa tggtttctga
    80221 ttggcatcgg gatgctggta tcggcgttca tcctgctggt gccgatgatt tatatcttcg
    80281 tacaggcgtt cagcaaaggg ctgatgccgg ttttacagaa tctggccgat ccggatatgc
    80341 tgcacgccat ctggctgacg gtgatgatcg cgctgattgc cgtaccggta aacctggtgt
    80401 tcggtattct gctggcctgg ctggtgacgc gctttaactt ccccggacgc cagttactgc
    80461 tgacgctact ggacattcct tttgccgtgt caccggtggt tgccggtctg gtgtatctgc
    80521 tgttctacgg ctccaacggc ccgctcggcg gctggctgga tgaacataat ctgcaaatta
    80581 tgttctcctg gcccggaatg gtgctggtca cgatcttcgt gacgtgtccg tttgtggtgc
    80641 gcgaactggt gccggtgatg ttaagccagg gcagtcagga agacgaagcg gcgattttgc
    80701 ttggcgcatc cggctggcag atgttccgcc gtgtcacatt gccgaacatc cgctgggcgc
    80761 tgctttatgg cgtagtgctg accaacgccc gcgcgattgg cgagtttggc gcggtgtcgg
    80821 tggtttccgg ctcgattcgt ggcgaaacac tttcgctgcc gttacagatt gaattgctgg
    80881 agcaggacta caacacagtc ggctccttta ccgccgccgc gctgttgacg ctgatggcga
    80941 ttatcaccct gtttttaaag agtatgttgc agtggcgcct ggagaatcag gaaaaacgcg
    81001 cgcagcagga ggaaaatcat gagcattgag attgccaata ttaagaagtc gtttggtcgc
    81061 acccaggtgc tgaacgatat ctcactggat attccttcag gtcagatggt cgcgttgctg
    81121 gggccgtccg gttccgggaa aaccacgctg ctgcgcatta tcgctgggct ggagcatcaa
    81181 accagcgggc atattcgctt ccacggcacc gacgtgagcc gcctgcacgc acgtgatcgt
    81241 aaagtcggtt tcgtgttcca gcattacgcg ctgttccgcc atatgacggt gttcgacaat
    81301 atcgcttttg gcctgacggt gctgccgcgt cgcgagcgcc cgaatgccgc agccatcaaa
    81361 gcgaaagtga caaaattgct ggaaatggtc cagcttgccc atctggcgga tcgttatccg
    81421 gcgcagcttt ccggcggcca gaaacagcgt gtagcgctgg cgcgtgcgct tgctgtagaa
    81481 ccgcaaattc tgctgcttga tgaaccgttt ggcgcgctgg atgcgcaggt gcgtaaagag
    81541 ctgcgtcgat ggctgcgtca actccatgaa gaactaaaat tcaccagcgt ttttgtgacc
    81601 cacgatcagg aagaagcgac cgaagtagct gatcgtgtag ttgtgatgag ccagggcaat
    81661 attgaacagg ctgacgcgcc ggatcaggta tggcgcgaac cggcgacccg ttttgtgctc
    81721 gaatttatgg gcgaagtgaa ccgcctgcag ggaaccattc gcggcgggca gttccatgtt
    81781 ggcgcacatc gctggccgct gggctacaca cctgcgtatc aggggccggt ggatctcttc
    81841 ctgcgcccgt gggaagtgga tatcagccgc cgtaccagcc tcgattcgcc gctgccggta
    81901 caggtactgg aagccagccc gaaaggtcac tacacccaat tagtggtgca gccgctgggg
    81961 tggtacaacg aaccgctgac ggtcgtgatg catggcgacg atgccccgca gcgtggcgag
    82021 cgtttattcg ttggtctgca acatgcgcgg ctgtataacg gcgacgagcg tatcgaaccc
    82081 cgagatgagg aacttgctct cgcacaaagc gcctgatagg ttgagtgaat gttaaacgcc
    82141 cggaggcgct tcccgcgatc cgggcttttt aatggcaagg tttgtaacct gtaggcctga
    82201 taagacgcgc aagcgtcgca tcaggcaaca ccacgtatgg atagagatcg tgagtacatt
    82261 agaacaaaca ataggcaata cgcctctggt gaagttgcag cgaatggggc cgaataacgg
    82321 cagtgaagtg tggttaaaac tggaaggcaa taacccggca ggttcggtaa aagatcgtgc
    82381 ggcgctttcg atgatcgtcg aggcggaaaa gcgcggggaa attaaaccgg gtgatgtgtt
    82441 aatcgaagcc accagtggta acaccggcat tgcgctggca atgattgccg cgcttaaagg
    82501 ctatcgcatg aaattgctga tgcccgacaa catgagccag gaacgccgtg cggcgatgcg
    82561 tgcttatggt gcggaactga ttctggtcac caaagagcag ggcatggaag gtgcgcgcga
    82621 tctggcgctg gagatggcgg atcgtggtga aggaaagctg ctcgatcagt tcaataatcc
    82681 cgataaccct tacgcccatt acaccactac cgggccggaa atctggcagc agactggcgg
    82741 gcgcattacc tattttgtct ccagcatggg aacaaccggc actatcaccg gcgtttcacg
    82801 ctttatgcgc gaacaatcca aaccggtgac cattgttggc ctgcagccgg aagagggcag
    82861 tagcattccc ggcattcgcc gctggcctgc ggaatatctg ccggggattt tcaacgcttc
    82921 tctggtagat gaggtgctgg atattcatca gcgcgatgcg gaaaacacca tgcgcgaact
    82981 ggcggtgcgg gaaggaatat tctgtggcgt cagctccggc ggcgcggttg ccggagcact
    83041 gcgggtggca aaagctaacc ctggtgcggt ggtggtagcg atcatctgcg atcgtggcga
    83101 tcgctacctt tccaccgggg tgtttgggga agagcatttt agtcaggggg cggggattta
    83161 agattattgc gcaggcaccg ttttatccgt ctgcgcaatt aacgcatgta aaaaggctgt
    83221 gcattctcat cgctcttacc ggggatcttc acaatatacg gcttcccggt aatcgatgac
    83281 gacgaagcaa ccttatcaat aaactgctcg gcagtgtcaa tgcggttacg ggtattgccc
    83341 agcttcagac gcagatgaga aaccgcttca tcgcaggtat gttcatcacc gttgcgcaca
    83401 aatatcaaat cctttttctg tgctaatccc tccagcatgg cgttgatgcg ggcttcttca
    83461 tgggcagtta acttcgcgta agcggggagt gtcattagta gtgtaatgac cagacaaatg
    83521 attttcttca ctgaattacc ttatccttgt tttgtaatag ccgatttgac tgccagaatc
    83581 aggtaaagtt tcggttggaa tacgatgagg atcatcggca aggagaaaaa attaaatgag
    83641 tagtttgttg ttgtttaacg ataagagcag ggcgctgcag gcggatatcg tcgccgtgca
    83701 gtcgcaggtg gtttacggca gcgtgggcaa cagcattgcc gtgcctgcta tcaaacagaa
    83761 cggcctgaat gtctttgccg tgccgacggt attgctgagc aatacgccgc attatgacac
    83821 tttctacggt ggtgcgattc cggatgaatg gtttagcggc tatttgcgtg cacttcagga
    83881 gcgtgatgcg ctgcgccaac ttcgtgctgt aaccacgggc tatatgggaa cggcatcgca
    83941 aatcaaaatc cttgccgagt ggctgactgc gctacgcaaa gaccatcctg acctattgat
    84001 catggtcgat ccggtgattg gcgatattga cagcggaatt tatgtcaaac ctgaccttcc
    84061 cgaagcgtat cgacaatatt tactgccgct ggcgcaggga attaccccca atatctttga
    84121 gttggaaatc ctgaccggta aaaattgccg cgatctcgac agtgccattg ctgccgcaaa
    84181 aagtctgctt tcagacacac taaaatgggt ggtgattacc agcgcctccg gtaatgaaga
    84241 aaatcaggag atgctggttg tggtggtcac tgccgacagc gtgaatgtca tttcccattc
    84301 aagggtaaaa accgacctga aagggactgg cgacctgttt tgtgctcagc ttatcagtgg
    84361 tttgctgaaa gggaaggcat taaacgatgc ggtgcaccgc gcggggttgc gcgtactgga
    84421 agtgatgcgc tacacccagc agcatgagag cgatgaattg attttgccgc cgctggcgga
    84481 agcataaaaa aatggcgccg atgggcgcca tttttcactg cagcaagaat tacttcttga
    84541 tgcggataac cggggtttca cccacagtca cgctaccgga cagtttgatc agttctttga
    84601 tttcgtccat gttggagata acaaccggag tcagggtaga cttggctttc tcttccagca
    84661 gcggcagatc aaattcaatg acagtatcgc caactttcac gcgctgacct tcttcagcaa
    84721 tacgcttgaa gccttcgcct ttcagttcaa cggtgtcgat accgaagtgg acgaacagtt
    84781 caacgccgct atcagattcg atagagaatg cgtggttggt ttcaaagatt ttaccaatgg
    84841 tgccgtctac aggcgcgacc attttgttac ccgttggttt gatagcaata ccatcaccaa
    84901 cgattttttc cgcaaaaacg acatccggca cgtcttcgat attgacgatc tcgccagaga
    84961 gcggagcaat gatctcaata gttccggtat ccttcttgtc gtcggaaacc agagatttca
    85021 gtttatcgaa caaacccatg atcttctcct aagcagtaaa ttgggccgca tctcgtggat
    85081 tagcagattg ttttttcttc aatgaacttg ttaaccagcg tcattaactc gtccgttgtc
    85141 ggttgagcaa gagcctgctc tgctaacacc ttcgcatctt cgaagttcgt gttacggata
    85201 atcttcttaa tgcgcgggat agaaatggcg ctcatagaga attcgtccag acccatcccc
    85261 agcaacagaa gtgtagcacg ttcatcgcca gcaagctcac cacacatgcc agtccatttg
    85321 ccttcagcat gagaagcatc aataacttgc ttgatcaagt tcagcacgga cggtgacatt
    85381 ggctggtaaa ggtgtgaaat catatcatta ccacggtcaa ctgccagagt gtactgcgtt
    85441 aaatcattgg tgccgatact aaagaaatca acttctttgg ctaaatgacg tgcaattgtt
    85501 gcggcagccg gtgtttccac cattacgccg atttcaattg actcgtcaaa cgctttacct
    85561 tcgtcgcgca gttcctgttt gtagatttcg atctctttgc gcagtgcacg cacttcttca
    85621 acagagatga tcatcgggaa cataatgcgc aatttaccga aagccgaggc acgcaggata
    85681 gcgcggagct gatcgcgcag gatctcttta cgatccattg cgatacggat agcgcgccag
    85741 ccgaggaacg ggttctcttc tttcgggaag ttcatgtatg gcagctcttt gtcgccgccg
    85801 atgtccatgg tacgaacgat aaccgcctgc gagccacacg cttcagccac tgctttgtaa
    85861 gcagcaaact gttcttcttc agtgggcagc gcgtcgcggt ccatgaacag gaactcagta
    85921 cgatacagac caacgccttc agcgccgtta cgctctgcac cttcaacgtc acgaaccgta
    85981 ccaatgttag cgcatacttc tacttggtga ccgtccagcg taatagctgg cagatctttc
    86041 agtttagcaa gctctgcttt ttcagaagcc acttgctcct gaacagcgcg cattttatca
    86101 ataacttcgt tggttggatt gacgtaaacc tgattattta cggcatccag aatcagatag
    86161 tcgtcatttt tcacctgaga ggtgacgcta ccggtaccca cgatagcagg cagttccaga
    86221 gaacgcgcca tgatagaggt gtgggaagta cggccacccg cgtcggtgat gaaacccagc
    86281 accttcttca ggttcagctg tgcggtttcg gacggcgtca ggtcagcggc aaccagaatg
    86341 acttcatcct gaatggcgct caggtcgata atcttcaggc ccaggatgtt gcgcagcagg
    86401 cgcttaccga tatcacgtac gtcagccgca cgttctttca ggtattcatc atccagctct
    86461 tccagggcag aagcctgacc ttcgataact tcatgagcag ctgcgtcagc tgtcatgtgt
    86521 ttatctttaa tcagggctat gatttcctgc tccagctcct catcttcgag cagcataata
    86581 tgcccttcaa agatggcttc tttttcttca ccgaacgttt caccagcttt cgttttgatc
    86641 gtttccagct gggctgatgc cttggcacga ccgctcagaa aacgttcaac ttcctgatca
    86701 acctggtcgg cagaaatttt tttccggtca atgacaattt cgtcttcttt cagaagcaga
    86761 gctttaccga aagcgatacc cggggatgct aaaatgcctg aaatcataac cctaccttac
    86821 ttgtgactga tttttaaaag aacccgggaa attactcgag ttccgccatc agtttaacca
    86881 gatgttcaac cgctttctgc tcgtcttcgc cttctgcgga gatagtcaca actgtacctt
    86941 gagtcaggcc cagagtctgc agtttaaaca ggcttttcgc gctggcgctt ttgccgttgg
    87001 aagtcacagt aatttcagaa gtgaagccct tagcttcttt tacaaactgg gcagcagggc
    87061 gggtgtgcag accgttcgga gcggtaatgg taacttcttg ctggaacatt gtatttcccc
    87121 aacttatagg tttagtgttg tggaactaaa gtctagcctg gcggttcgac tttagcctgt
    87181 attgttagcg ccagttttta acagacgcga cgcacgaaga gcgcaataca gtccattgct
    87241 ggcgggtgtt gaccgctgac gtttcgtacg tcattaaaca ttatgccgct aaaggaagaa
    87301 ttgaaccaaa tcataaaatc gattcagctt tggcttttcc tgtaacgatt aatttcgcgc
    87361 atcaaaataa ttagtctggt taaataccag ttccgacagg ctgaatcaat gccaggaggg
    87421 gcgaaagttt gaagcaggcc acaaaaaagc acctgaaaag gtgctttttt acgcattttt
    87481 aacaagctgg cattactgtt gcaattcttt ctcagtgaag agatcggcaa acaatgcggt
    87541 gcttaaataa cgctcacccg atgacggtag aataaccaca atattcttgt tggtaaagct
    87601 ttcatcttct tgtagtttca acgccgcagc aacagctgct ccagaagaga tacctgcaag
    87661 aataccttct tcttccatca gacgacgcgc ggtagaaatc gcttcttcat tggtgatgcc
    87721 aatgacttta tcgaccagct tgagatcgag gttagccggg ataaaaccag cgccaatacc
    87781 ctgaatttta tgcgggccag gtttaatctc ttcacctgcc agcgcctggg cgataactgg
    87841 agaatcggtt ggctcaacgg cgacagagat aagatcggtc ttacctttgg tgcctttaat
    87901 gtagcggctg acgccagtca gcgtaccgcc agtcccaacg ccagcaataa atacatcaac
    87961 ctgaccgtcg gtatcttccc agatctccgg accggtggtc ttttcgtgaa tttcagggtt
    88021 tgccggattg ctgaattgtt gcagcagcag gtatttctct ggattgctgg cgacaatttc
    88081 ttctgctttt tggattgcgc ctttcatgcc tttagcacct tcggtcagca ccaggtttgc
    88141 acctaacgct ttcagcagct tgcggcgttc aatactcatg gtttctggca tggtcagggt
    88201 gagtttgtaa ccgcgagcgg cagctacata ggccagtgca atcccggtat taccgctggt
    88261 tggttcaacc agttcaacgc ctggtttcag cacgccgcgc ttttcggcat cccaaatcat
    88321 gttggcaccg atacggcact taacgctgaa gctggggtta cgagattcca ccttcgccag
    88381 aatgcgtccg ttaccgatgc gattcaggcg aaccagcggc gtgtgaccga tagtcagcga
    88441 gttatcttca aaaatcttac tcatggcctg tccttaactg tatgaaattg ggatacaaca
    88501 ggtagcatac ccgctcagag aatatgcgga agtaaggatt tagcatatct atatgcagaa
    88561 gggaaataat gacatgcaag atggaataag gggcggcata agccccccct gtttcacaca
    88621 aacggtttac aaaataaccg gtagattgtt accgccacat cgcgtgttta tcgcgatagc
    88681 aatcgaccca catcgccgtc gcgccacaaa cggcaacggg cataatgaac agattaagca
    88741 gcgggatcat cgtaaacaga ctggttaaag caccaaactg catattggtg attttgcgtg
    88801 tgcgcagggc ggtgcgcatc tctttaaacg gcactttgtg gttatcgaag gggtaatcgc
    88861 aatactggat ggctaacatc caggcgctaa acaggaacca cagtaccggc gcgacggttt
    88921 gcccaatacc agggatgaag taaagaatta gcaatacaat tgcgcgtggc agataccagg
    88981 caaatttttg ccattcgcgt ttcatgattc gcggcacatc tttcatgata ccgaaaatcc
    89041 cggtatctgg cggtgtagcg ccagtcaatc gtgcttccag ttgttcagcc aataaaccgt
    89101 taaacggagc ggcaatccag ttagcaatcg tggagaagaa atagccaaac actaacagca
    89161 cagagatgac agccagaggc cacaacagat agctcagcca ttgtaaccag tccggaacat
    89221 aactcatgag agtcgggatc cagacatcga gttgcgtaaa gagccaccag aatgcgcccc
    89281 ccatcaacaa aatattgacc agcagcggta aaataacgaa acgccgaatc ccaggttgcg
    89341 agacgagctt ccagccttgc gcaaaatagt aaaaaccgct gcgtggggca gatgtgaatg
    89401 atgaaaccat aatcaggatg agctcctttt gaccaatccc aggaaaattg tgcgtatttt
    89461 accgggtaat tgcgcaatgg acagttagga tatgttcgaa aaaacagcaa aaagcacgat
    89521 ttcatctatc tttgtgctgt gaaagttaat agtgcacttg cacttgaggt aatcggcaaa
    89581 tactcttagt gagtaaatgt ttgccgtggt ggcaaggtgt tagaacaaca gagaatataa
    89641 tgatgcagga tttgcgtctg atattaatca ttgttggcgc gatcgccata atcgctttac
    89701 tggtacatgg tttctggacc agccgtaaag aacgatcttc tatgttccgc gatcggccat
    89761 taaaacgaat gaagtcaaaa cgtgacgacg attcttatga cgaggatgtc gaagatgatg
    89821 agggcgttgg tgaggttcgt gttcaccgcg tgaatcatgc cccggctaac gcccaggagc
    89881 atgaggctgc tcgtccgtcg ccgcaacacc agtaccaacc gccttatgcg tctgcgcagc
    89941 cgcgtcaacc ggtccagcag ccgcctgaag cgcaggtacc gccgcaacat gctccgcgtc
    90001 cagcgcagcc ggtgcagcaa cccgtgcagc agcctgccta tcagccgcag cctgaacagc
    90061 cgttgcagca gccagtttcg ccacaggtcg cgccagcgcc gcagccagtg cattcagcac
    90121 cgcaaccggc acaacaggct ttccagcctg cagaacccgt agcggcacca cagcctgagc
    90181 ctgtagcgga accggctcca gttatggata aaccgaagcg caaagaagcg gtgattatca
    90241 tgaacgtcgc ggcgcatcac ggtagcgagc taaacggtga actgcttctt aacagcattc
    90301 aacaagcggg cttcattttt ggcgatatga atatttacca tcgtcatctt agcccggatg
    90361 gcagcggccc ggcgttattt agcctggcga atatggtgaa accgggaacc tttgatcctg
    90421 aaatgaagga tttcactact ccgggtgtca ctatctttat gcaggtaccg tcttacggtg
    90481 acgagctgca gaacttcaag ctgatgctgc aatctgcgca gcatattgcc gatgaagtgg
    90541 gcggtgtcgt gcttgacgat cagcgccgta tgatgactcc gcagaaattg cgcgagtacc
    90601 aggacatcat ccgcgaagtt aaagacgcca acgcctgata cacttaaggc aaattaactc
    90661 ctcttcgaac ccccgcttgt cgggggtttt tagcattgat ggtgcgatat ggaatcaatc
    90721 gaacaacaac tgacagaact gcgaacgacg cttcgccatc atgaatatct ttatcatgtg
    90781 atggatgcgc cggaaattcc cgacgctgaa tacgacaggc tgatgcgcga actgcgcgag
    90841 ctggaaacca aacatccaga actgattacg cctgattcgc ctacccaacg tgtaggtgct
    90901 gcgccgctgg cggctttcag ccagattcgc catgaagtgc caatgctgtc gctggataac
    90961 gtttttgatg aagaaagctt tcttgccttc aacaaacgtg tgcaggaccg tctgaaaagc
    91021 aacgagaaag tcacctggtg ctgtgagctg aagctggatg gccttgccgt cagtattctg
    91081 tatgaaaatg gcgttttagt cagtgccgcg actcgtggcg atggcaccac cggggaagat
    91141 atcacgtcta atgtgcgtac tattcgcgcc attccactga agttgcacgg agagaatatc
    91201 ccggcgcgtc tggaagtgcg tggtgaagtg ttcctgccgc aggcggggtt cgaaaagatt
    91261 aacgaagatg cgcgacgcac gggcgggaaa gtgtttgcta acccacgtaa tgcggcagct
    91321 ggttcactgc gtcagcttga tccgcgtatt acagcgaagc gaccgctcac ttttttctgc
    91381 tatggcgttg gtgttctgga aggtggcgag ctgccggata ctcatcttgg ccgtttactg
    91441 caatttaaaa agtgggggtt gccggtcagc gatcgggtaa cgctttgtga atcggcggaa
    91501 gaagtgctgg cgttctatca caaagtggaa gaagaccgcc cgacgctggg ctttgatatc
    91561 gacggcgtgg tgattaaggt caactcactg gaacagcagg agcagcttgg ctttgtcgcg
    91621 cgagccccgc gctgggcggt agcgtttaaa ttcccggcgc aggaacagat gacctttgtg
    91681 cgtgacgtcg agtttcaggt tgggcgtact ggcgcgatta cgcctgttgc gcgtctggaa
    91741 cctgtccatg ttgcaggcgt gctggtgagt aacgcaacct tacacaatgc ggatgaaatc
    91801 gaacgtcttg gtttacgcat tggcgataaa gtggtgattc gccgcgctgg cgacgtgatc
    91861 ccgcaggtgg ttaacgtcgt gctttctgaa cgcccggaag atacccgtga ggttgtattc
    91921 ccgacgtatt gtccggtatg tggttctgac gttgagcgtg tggaaggtga agcggttgcc
    91981 cgctgtactg gtggtctgat ttgcggtgcg cagcgtaaag agtcgctgaa acactttgtt
    92041 tcccgccgtg cgatggatgt tgacggaatg ggcgacaaaa tcatcgatca gctggttgaa
    92101 aaagaatatg tccacactcc ggcggatctg ttcaaactca ccgcaggcaa actgaccgga
    92161 ctggagcgta tggggccaaa atcggcacaa aacgtggtta acgcgctgga aaaagcgaaa
    92221 gaaaccacct ttgctcgctt cctctatgca cttggcatcc gtgaagtcgg cgaggccacc
    92281 gcagcaggtc tggcggcata tttcggaacg ctggaagcgc tggaagccgc ttcgattgaa
    92341 gagctgcaaa aggtgcctga tgttggcatt gtcgttgcat cccacgttca caacttcttt
    92401 gccgaagaaa gcaaccgcaa tgtcatcagc gagctgttgg cggaaggtgt tcactggcct
    92461 gcgccgatcg ttatcaacgc ggaagagatt gacagcccgt ttgctggtaa aaccgtggtg
    92521 cttacgggca gcttaagcca gatgtcgcgt gatgacgcta aagctcgact ggtcgaactg
    92581 ggcgcgaaag tcgcgggcag cgtgtcgaag aaaaccgatc tggtgatagc gggtgaagct
    92641 gcaggatcta aactggcgaa ggcgcaggaa ctgggcattg aagtcatcga cgaagcggaa
    92701 atgctgcgtt tgctgggtag ctgagatgga aaaagagcag ctgattgaaa tagccaatac
    92761 gataatgccg tttggcaaat acaaagggcg tcgcttaatc gacctgccgg aggagtatct
    92821 gctgtggttt gcccgcaaag atgagttccc ggcagggaag ctcggtgagc taatgcaaat
    92881 cacgctgctg attaaaaccg aggggctgac gcaactggtc cagcccctga agcgtccgct
    92941 ttaagcttta tcggcgctgc tttcctgctg cgcctgtaac tgttcggtct ggcgtttgta
    93001 tcgacgcgcc agcaccgcac agaccatcaa ttggatctga tggaaaatca tcaggggcag
    93061 caccatcata ccgatcaccg atgtggggaa cagaatgttt gccatcggga tgccatttgc
    93121 cagactcttt ttcgaaccac aaaagacgat agtaatttca tctgccttat tgaagcccag
    93181 tcggcgtgcc ataaagacgt taactacaat cacgatagcc agaagaacgc agctgaccac
    93241 cacgataaac agcaatgatc cccagccaac tttatgccag ataccattaa cgacggcttc
    93301 gctgaacgct gtataaacca ccaacagaat ggacgtctgg tcagttttcg caatccattt
    93361 tttattgcgc gacacccagt caccaatcca cggccgggaa agatgcccca acacaaacgg
    93421 cagcagcagt tgcagcataa ttttaccgac ctgctcaagg ctgccccctg caccgtgaac
    93481 attcatcacc agaccaacca gcaatggtga aaggaaaatc cccagcagac tggatgctga
    93541 cgcagaacaa accgccgccg cgacgttacc gcccgccatt gacgtgaagg cgattgcaga
    93601 ctgcacggta gccgggagaa tgcacaagta gagaaaaccg gagtagagca tcgggtcgac
    93661 atttaccggt ttccaccagg caaacagtac acccagaatc ggaaacagca cgaaggtgct
    93721 gcacattacc cacaaatgca gtcgccagtg accaccgcca gcaataatcg cctcacgcga
    93781 caacttcgcg ccgtgcataa agaacagcag ggcaatagct gcggtggtca gattttcaaa
    93841 gaaggggacg aaatcgcctc tggcaggaaa gaaagaggcc agcaacacca ccgtgatcag
    93901 ggttaaggtg aaaggatcga ggatacgaaa aagtttcata aagactccag atgatcgatg
    93961 gttctattgt gcttcttcca gtttgagaaa taaaattgat ttattgcatc cattcatgaa
    94021 gaaaacagat gaattattct ttaaaacaat taaaggtttt cgtcacagta gcgcaggaga
    94081 aaagttttag tcgtgcagga gagcgtatcg gcctgagcca gtcggcagta agtcacagtg
    94141 tgaaggagct ggaaaatcat actggtgttc gcctgctgga cagaaccacg cgtgaagtgg
    94201 tgcttacaga tgcagggcag cagttggctt tgcgtcttga gcgactgctg gatgaactta
    94261 acagcacgtt gcgcgatacc gggcgtatgg ggcaacaact gagcggaaaa gttcgggtcg
    94321 ctgccagcca gaccatttcc gcgcatctta ttccgcaatg cattgctgaa agccatcgcc
    94381 gctatccaga tattcagttt gtcctgcacg atcgtccgca gcagtgggtg atggaaagta
    94441 ttcgccaggg agatgtcgac tttggcatcg tcatcgatcc aggcccagtg ggcgatctgc
    94501 aatgtgaagt gattctttcc gagcctttct ttctgctttg ccatcgggat agcgctttgg
    94561 ccgcggaaga ttatgtaccc tggcaggcat tacagggagc gaaattggtg ttgcaggatt
    94621 atgcgtcagg cagccgaccg ctgattgacg cagcgctggc gcgcaatggt attcaggcga
    94681 atattgtgca ggagattgga catccggcga cgctgttccc gatggtagcc gcaggcatcg
    94741 gcatcagtat tcttcccgca ctggcgctac cattgcctga aggtagcccg ctggtggtaa
    94801 agcgcattac gccggtggtg gaacgccagt tgatgctggt gcgtaggaaa aaccgttcac
    94861 tttccaccgc cgctgaagcg ttatgggacg tggtacgaga ccagggcaat acgctaatgg
    94921 ctgggcggga aggggatccg ttgtatcaga tatagatacc gatatgagtc gtattttcaa
    94981 caggatttag aagggataca ttcagagaca aacgctgctc tttctcctgc gccttttctg
    95041 cctgctgtct ctgcaactgc gccagttgtg tttccagtag ggtgatttgc tgttgaatca
    95101 attcggcctg tttttgcttg tcttcagcac tgccgctagt gtctacaatc tctttaatgt
    95161 gttgagtgag tttgatgatt tgctgactta tccgcgcgat ttgtgaagca acatcgctac
    95221 cttgtgatgc cctgttacta ctctgtgttt gtatggaagg aattgaagtt gaagagatag
    95281 tggccattgt tcagtcctcg tcactgtacg aggtaatatc ggcagaagaa caaggaactt
    95341 gagaagaaaa atcaatctta caaatcgcag atagcaaaaa agcgccttta gggcgctttt
    95401 ttacattggt gggtcgtgca ggattcgaac ctgcgaccaa ttgattaaaa gtcaactgct
    95461 ctaccaactg agctaacgac ccgaagtggt gggtgatgac gggatcgaac cgccgacccc
    95521 ctccttgtaa gggaggtgct ctcccagctg agctaatcac ccacttcggt acttcacatt
    95581 tttacaagaa atttagctgg tgagaaagtg gtgggtgatg acgggatcga accgccgacc
    95641 ccctccttgt aagggaggtg ctctcccagc tgagctaatc acccacttct caatttcttg
    95701 ctacacggcg gagactacat aaagtagttg gtgggtgatg acgggatcga accgccgacc
    95761 ccctccttgt aagggaggtg ctctcccagc tgagctaatc acccccgctg tgtggagtcg
    95821 cattataggg agagttcaaa atgagtcaac gcattttcta aagaaattgt tcgttcgtcg
    95881 taaatttaag caagatgatc gcaaaacaga ccgtgttgcg caatttgtca acgaaaacaa
    95941 taatgcgtaa ggttgcaacc cgaactacat tgaggaatca ggcgggagtg atagaatatc
    96001 gcccactcaa tttttccagg atttgccggt tgtcggcatc tttctaaacg taaggccatt
    96061 tcatgaaaat caaaactcgc ttcgcgccaa gcccaacagg ctatctgcac gttggcggcg
    96121 cgcgtactgc tctttactcc tggctttttg cacgtaatca cggcggtgag ttcgtgctgc
    96181 gtattgaaga caccgatctt gagcgttcca cgccggaagc tatcgaagcc attatggatg
    96241 gcatgaactg gctgagcctg gagtgggatg aaggtccgta cttccagacc aaacgttttg
    96301 atcgctacaa cgcggtgatc gatcagatgc tggaagaggg cacagcttat aaatgctatt
    96361 gctctaaaga gcgcctggaa gcgctgcgcg aagagcaaat ggcgaaaggt gagaagccgc
    96421 gttatgacgg tcgctgccgc cacagtcatg agcatcacgc tgatgatgag ccgtgtgtcg
    96481 tacgttttgc taacccgcag gaaggttctg ttgtttttga cgatcagatc cgtggtccga
    96541 tcgagttcag caaccaggaa ctggacgatc ttattatccg ccgtaccgat ggttccccaa
    96601 cctataactt ctgtgtggtt gtcgatgact gggatatgga aatcacccac gttatccgtg
    96661 gcgaagacca tatcaacaac acgccacgcc agatcaacat tcttaaagcc ctgaaagcgc
    96721 cggtaccggt ttacgcgcac gtttctatga tcaacggcga tgacggcaaa aaactgtcca
    96781 aacgtcacgg ggcagtcagc gtaatgcagt atcgtgatga cggttatttg ccagaagcac
    96841 tgctgaacta tctggtgcgt ctgggctggt cccacggcga tcaggaaatc ttcactcgtg
    96901 aagagatgat caaatacttc actttgaatg ccgtcagcaa atctgccagt gcgttcaaca
    96961 ccgacaagct gctgtggctg aaccatcact acattaacgc gctgccgccg gagtatgttg
    97021 ctactcactt acagtggcac attgagcagg aaaatatcga tacccgtaac ggcccgcagc
    97081 tggctgatct ggtgaaactg ctgggcgaac gctgcaagac gctgaaagag atggcacaga
    97141 gctgccgtta tttctacgaa gattttgctg agttcgatgc cgacgccgcg aaaaaacatc
    97201 tgcgtccggt agcgcgtcag ccgctggaag tggttcgtga caaactggcc gcgattactg
    97261 actggacagc tgaaaacgtt catcacgcta ttcaggcgac ggcggatgag ctggaagtgg
    97321 gtatgggtaa agttggtatg ccgctgcgtg tcgccgtaac cggtgcgggg cagtctccgg
    97381 cgctggacgt taccgttcac gcgatcggta agacccgcag tatcgagcgt atcaacaaag
    97441 cgctggcttt tattgcggaa cgcgaaaatc agcagtaatt agcgcataaa agataaacgg
    97501 caggagagat acccctgccg ttttttattt atccggtgcg ccgtaaaacc ccgtccttca
    97561 gggcggggat ataaggcgcg gttttccacc tgaccaggtg tttgctgttg ttcaatgtat
    97621 tggcggatga cggatattgg cgcacctccg cagctactgg caaaatagcc aggactccac
    97681 aaaacgcctt tgtagtaata cctgactgca atatctggtc gatctcgtcg cagtaaccta
    97741 cccgatacgc ctttgaggct gtttaccaga ctggatatcg ccagtttggg aggatagtta
    97801 gttaacaaat ggacgtgatc tggttcgcca tccatttcaa ccagttcagc ttcaaaatca
    97861 gcacatacat ttgaaaagta agtgcgtagt ttttctgtcg cgtcatagtc aaaaatctga
    97921 cgtcggtatc tggtgacaaa gaacagatga acatgcatca ggaaaacaca atgccttccc
    97981 cgtcggatat cggtttcttt attcatagac caaagtatca tttggcctgt gaaacgacta
    98041 caggcattta aattccagtt aagaccaggt ggtcaacagg agcgtgaaat gaggcgcttc
    98101 gcaggcgcat gtcgtttcgt tttcaatcgt gctctggcgc ttcagaatga gaatcatgag
    98161 gccggtaata aatacatccc ttacgggaaa atggcttcct ggctggttga gtggaaaaac
    98221 gccactgaaa cgcaatggct taaagatgcc ccgtcacagc cattgcaaca gtcactgaaa
    98281 gagcttgagc gggcttacaa aaacttcttc cggaagcggg ctgcttttcc ccgattcaaa
    98341 aagcggggac agaatgatgc attccgctac ccgcagggcg ttaagctcga tcaggaaaac
    98401 agccgtattt ttctgccgaa acttggctgg atgcgctacc tgaacagccg tcaggtcacg
    98461 ggtgttgtga aaaatgtcac tgtcagccag tcctgcggta agtggtacat cagtattcag
    98521 acagaaagtg aagtatcaac tccggttcac ccttcagcat caatggtcgg actggatgct
    98581 ggcgtggcta aactcgccac gctgtcagat ggcacagtct ttgagcctgt aaacagtttt
    98641 cagaaaaacc agaagaagtt ggcgagactt cagcgacagt taagccgcaa ggtcaaattc
    98701 agcaataact ggcagaagca gaaacgcaaa atacagcgac tgcattcctg taccgcaaat
    98761 atccgcaggg actaccttca caaagtcaca acgaccgtca gcaaaaacca cgcaatgata
    98821 gtcattgagg atttgaaggt cagcaacatg tcaaagtcag cagcgggtac ggtcagccag
    98881 ccggggcgca atgtccgggc aaaatcaggt ttaaaccgtt cgatactgga tcagggctgg
    98941 tatgaaatgc gccgccagct tgagtacaag cagctctggc gtggcggtca ggtactggct
    99001 gttccgccag cgtacacaag ccagcgttgc gcgtgctgtg gtcatacagc gaaagaaaac
    99061 cgcctgtcac aaagtaaatt cagatgccag gtatgtggat atacagcgaa cgccgatgta
    99121 aacggcgctc gtaacatttt agcggcgggg cacgccgttc ttgcctgtgg agagatggtg
    99181 cagtcaggcc gctcgttgaa gcaggaaccc accgaaatga ttcaggcgac agcctgaacg
    99241 tagcagggat cctcgtcatt cagggcgtgg aggatgtcaa gctgattcat ctatgtcgag
    99301 acgagcaagg aaattacgaa ttccttcgcg tgacaaaaat tgcgcaactt tttcctgctc
    99361 actgtgagtc atgttgtcta ttgcactgat gatttgccga taagcaggag tgcgaatccc
    99421 cggtgcgtat tcggcaaggg gttcttcggc ttccagaagc atcggtgtgt tatggaaggc
    99481 tggcgtgttc tgaataaact cgcaaacttg cgtatcgacc agaataagtc tggcacgacc
    99541 gcctttcacg ccgggaaatt tttccgtttt ccagcctttt tctctgatcc aacggttaac
    99601 agtttgttta gccacaccga ggcattcagc cagttcttcg gtggtcattt tattgcgtaa
    99661 tcttttcata ttatttgcta tcgcgaatcc ctaagcgttg taacaatccg ataatccctt
    99721 cccgcagaag caaggatgta aactgttttt gttcaactgg cgtcatctct ttcgccagtg
    99781 tcaccagcaa tacttcaagc ggcgcatcac cagaaagggc aggcgcttct ccctgacctt
    99841 ctggacgttc agcattacga atatattcac gaacctgttc attgacgtga accagtcggg
    99901 ctttgccacc ctggacgcca ggttttggtg acgttgtcca gccttccttg cgtacccatt
    99961 tattaatggt ctggcggcta tagccggtca gtctggcaag ttcatctggt gtcatcctct
   100021 ccttgaacat aacaattccc tgaatgtatg tgggtctatt agtagttcat tttatagcac
   100081 cactttacag gaaaccgtga catgaataac tactgaatgc gagcggtttt ccaaaattgt
   100141 tcatttgcct gatttttcag cgattgaaac tacacgttgc aatttgccgt tgacacattc
   100201 gggcggaatt catatgatgc cgcccgtcaa ctcgacaagc tttacgtgac ggggctatag
   100261 ctcagctggg agagcgcttg catggcatgc aagaggtcag cggttcgatc ccgcttagct
   100321 ccaccaaatt ttgcacccag caaatttggt acgtaaacgc atcgtggggc tatagctcag
   100381 ctgggagagc gcttgcatgg catgcaagag gtcagcggtt cgatcccgct tagctccacc
   100441 aaatttccaa ccctcgctgc aaagcggggg ttttttgtct ctgctttttg ccgcttttgt
   100501 aatacagtct acgtccgggt tagtgccgcc tggtgaaagc atcattggat gaaaaatcgg
   100561 caacaggatg gcccctgttt gcttcgcaat gcgaataaac ttattatttg tgtgcctgaa
   100621 aaccccgatc agtgagagta gtgtactcat gtttgtggag cataacctga taaaaaatat
   100681 caagatattc acactagcgt ttacgctcac cgtggtactt attcagctat cccgttttat
   100741 ttcgccactt gccattatcc attccagtta tatctttctg gcgtggatgc cactgtgcgt
   100801 aatgctgtca atcttgttta tctttggctg gcgcggtgtc gttcccgttt tatgcgggat
   100861 gttttgcacc aatctgtgga actttcatct ctctttttta cagactgcgg tcatgctcgg
   100921 tagccagacg tttgtcgtgt tgtgtgcctg cgcaatatta cgctggcagc tggggacgcg
   100981 ttggcgttat ggattgacca gccgatatgt ctggcaacgt ctgttctggc ttggtttggt
   101041 gacgccgatc ggcatcaaat gcagcatgta tcttgtgggg agcttctttg atttcccgct
   101101 aaagatatcc acctttttcg gcgatgcgga tgccattttc acggtcgttg atttgctaag
   101161 ccttttcacc gctgtgctga tttacaacat gcttttctac tatctcactc gcatgattgt
   101221 aagtccccac tttgcgcaga tattgtggcg cagggatatc gctccgtcgt tgggcaaaga
   101281 gaaacgcgca tttaccttaa gctggctggc agcgttaagc gtgctgctac ttctgttgtg
   101341 cacaccttat gaaaacgact ttattgccgg ttacctggta cccgttttct tcatcatctt
   101401 taccctcggg gtcggtaagc ttcgctatcc gtttttaaat ctcacttggg ctgtttcaac
   101461 gctttgcctg ctgaattaca accagaactt tttgcaaggg gtggaaaccg aatattcgct
   101521 ggcatttatt ctcgcggtgc tgatttcctt tagcgtttgc ctgctctata tggtgcgcat
   101581 ttatcatcgc agtgaatggc ttaatcgccg ctggcatttg caggcgctga ccgatccgtt
   101641 aacgctcctg cccaactttc gtgcgttgga acaagcgccg gagcaagagg cgggcaagag
   101701 tttttgctgc ctgcgcattg ataatcttga gtttatgagt cgtcattacg ggttgatgat
   101761 gcgtgttcac tgtatccgat caatttaccg tacgctgctg ccattgatgc aggaaaacga
   101821 aaagttgtat caattgccgg gtagtgaact gctgttagtg ctgagcgggc ctgaaacgga
   101881 agggcgactc cagcatatgg ttaacatcct gaatagtcgg caaattcact ggaacaatac
   101941 cgggctggat atgggctatg gtgctgcctg ggggcgtttt gatggaaatc aggaaaccct
   102001 gcaacccttg ttggggcagt taagctggct ggcggagcaa tcctgcgcac atcatcatgt
   102061 gctggcgctg gatagccgag aggagatggt ttccgggcag accactaaac aggtgctatt
   102121 gctgaatacc attcgcacgg cgttagatca gggtgatttg ctgctctacg cccagccaat
   102181 tcgcaacaaa gagggtgaag gttatgatga ggtcctcgcg cgactgaaat atgacggcgg
   102241 cattatgacc ccggataagt ttctgcccct tattgctcag tttaacctta gcgcgcgttt
   102301 tgatttgcaa gtgctggaat ccttgttgaa gtggctggca acacaccctt gcgacaaaaa
   102361 aggcccgcgc ttttcagtta atttaatgcc gctcacgctg ctgcaaaaga atattgccgg
   102421 gcggattatt cgtctattta agcgttatca catctccccg caggcggtca ttcttgagat
   102481 caccgaggag caggcgtttt ctaacgcaga aagcagcatg tacaacatcg agcagctgca
   102541 taagtttggt ttccggattg cgattgatga ctttggcacc ggctatgcca actacgaacg
   102601 gttaaagcgt ttgcaggctg atatcatcaa aattgatggc gtctttgtga aagatattgt
   102661 cacgaatacg ctggatgcga tgattgtgag atcaattacc gatctggcga aagcgaagtc
   102721 attgagtgtg gtcgcggagt ttgtcgagac gcaacagcaa caggcgctat tgcataagct
   102781 cggggtgcaa tatctgcaag ggtatttgat tggtcgcccg cagccattag ctgattaaca
   102841 ggcgtaaaaa agccggggaa ttatccccgg cttttttatg tatggtctta cagcaccagt
   102901 gctgcgattg acgcagacag cacactcacc agggtagagc cgtaaaccag cttcagaccg
   102961 aagcgagaaa ccacgttacc ttgctcttca ttcaggcctt taactgcacc tgcgataatc
   103021 ccgattgaag agaagttagc gaaggaaacc aggaacacag agatgatgcc ttcagcacgc
   103081 ggagagagtg tagaggcaat tttctgaaga tccatcatcg caacgaactc gttggaaacc
   103141 agtttggtcg ccatgatact gcccacttgc agtgcttcac tggaaggaac acccatcacc
   103201 catgcaatcg gatagaagat gtagcccagg atgccctgga aggagatgct gtagccaatc
   103261 cagccagtca cggtagcaaa cagtgcgttc agcgcggcga tcagggcgat aaagccaatc
   103321 agcattgcgg caacgataat ggcaactttg aaacctgcca gaatgtattc acccagcatt
   103381 tcgaagaagc tctgaccttc gtgcaggttg gacatctgaa tgttttcttc acaggcatca
   103441 acacggtaag ggttgatcag cgacagcacg ataaaggtgc tgaacatgtt cagtaccagc
   103501 gctgcaacga cgtattttgg ttccagcatg gtcatgtatg caccaacgat ggacatagaa
   103561 acggtggaca tcgccgttgc tgccatggtg tacatacggt tacgggagat tttgccaagg
   103621 atatctttat aggcaataaa gttttcagac tgacccagaa tcagggagct gacggcgtta
   103681 aaggattcca gtttgcccat gccgttgact ttggagagca ggaaaccaat tgcgcggata
   103741 atcaccggca acacgcgaat gtgctggaga ataccgatca gtgcagagat aaagacgatt
   103801 gggcacagca ctttcaggaa gaagaatgcc aggccttgat cattcatgct accaaagacg
   103861 aagttagtcc cttcgttggc aaatccgagc agtttttcga acatttcgga gaagcctttc
   103921 acgaagccta aaccaacgtc ggagttcagg aagaaccacg ccagtaacac ttcgataaca
   103981 agcagttgaa taacataacg gatacgaatt tttttgcggt cgctgcttac cagcagtgcg
   104041 aggatcgcaa caacggcaag tgccagtaca aaatgaagga cgcggtccat atttgctcca
   104101 aatatgaggc aggttaaatt tccgtgcaca ttctatgtaa catggttaag gaaaacgaga
   104161 tctgacacac actataatga cgtcctgtga caagcttcaa aaatagtgag ctgtcataca
   104221 tttctgctat gttgtgtatg gaagctgaaa gttatgtaaa tgtgctaaca ttaaacataa
   104281 ccataacatt caagtcatca atcttttcgt agtcataatc ctggtctatc agagaaatca
   104341 ccacaaccca tttaaatgaa tttgataatc attctcgttt ggcatagcat gaaacatagc
   104401 aaaggctatg ttttagaggc acaagatgac gaactatcgc gttgagagta gcagcggacg
   104461 ggcggcgcgc aagatgaggc tcgcattaat gggacctgcg ttcattgcgg cgattggtta
   104521 tatcgatccc ggtaactttg cgaccaatat tcaggcgggt gccagcttcg gctatcagct
   104581 actgtgggtt gtcgtttggg ccaacctgat ggcgatgctg attcagatcc tctctgccaa
   104641 actagggatt gccaccggta aaaatctggc ggagcagatt cgcgatcact atccgcgtcc
   104701 cgtagtgtgg ttctattggg ttcaggcaga aattattgcg atggcaaccg acctggcgga
   104761 atttattggt gcggcgatcg gttttaaact cattcttggt gtttcgttgt tgcagggcgc
   104821 ggtgctgacg gggatcgcga ctttcctgat tttaatgctg caacgtcgcg ggcaaaaacc
   104881 gctggagaaa gtgattggcg ggttactgtt gtttgttgcc gcggcttaca ttgtcgagtt
   104941 gattttctcc cagcctaacc tggcgcagct gagtaaagga atggtgatcc cgagtttacc
   105001 tacttcggag gcggtcttcc tggcagcagg cgtgttaggg gcgacgatta tgccgcatgt
   105061 gatttatttg cactcttcgc tcactcagca tttacatggc ggttcgcgtc aacaacgtta
   105121 ttccgccacc aaatgggatg tggctatcgc catgactatt gccggttttg tcaatctggc
   105181 gatgatggct acagctgcgg cggcgttcca cttttctggt catactggtg ttgccgatct
   105241 tgatgaggct tatttgacgc tgcaaccgct gttaagccat gctgcggcaa cggtctttgg
   105301 gttaagtctg gttgctgccg gactgtcctc aacggtggtg gggacactgg cagggcaggt
   105361 ggtgatgcag gggttcattc gcttccatat cccgctgtgg gtgcgtcgta cagtcaccat
   105421 gctgccgtca tttattgtca ttctgatggg attagatccg acacggattc tggttatgag
   105481 tcaggtgctg ttaagttttg gtatcgccct ggcgctggtt ccactgctga ttttcaccag
   105541 tgacagcaag ttgatgggcg atctggtgaa cagcaaacgc gtaaaacaga caggctgggt
   105601 gattgtagtg ctggtcgtgg cgctgaatat ctggttattg gtggggacgg cgctgggatt
   105661 gtagttgaat gagcgtcgca tctggcacta ttggcgatgc ggcgtaaacg ccttatccgc
   105721 cctacgcgtt aaagagccgg tttgtaggcc tgataagacg cactagcgtc gcatcaggcc
   105781 ttgtgctcca ttggcgaatg tgtttaatgg cggtgtccat gtccgcggcc ttttccgcga
   105841 tgatcgtcac gatcgcgcca gccttcacga tagccacgct catatgcttt acgcttatcc
   105901 cagccacgat ggtagccatt atcatgacgc caccagcggt ttttacgcca ttcataattg
   105961 cgatgccagt agtcacggtc gcgccagtga ccaccgtccc agtaattacc gtaatgatcg
   106021 cgatcgccaa tttgtaattt gattgatggc agtagggtga tttcgcctgc gtttgctgca
   106081 agcggggtaa atgccatcag ggcggccgcc agaaacagtg acctgaacat tgttattctc
   106141 cttcacgatc gaagccgtca gcggcctgtt aacgcaatat tacggggagg taaagccccg
   106201 tatcatcgcc ataactctta aatcataaag gagagcattt tttgctaaac gttatcgtgg
   106261 ggtggctgtt gcttcggacg attgaccatc atcatcggct tacctgctaa taacagccat
   106321 gccggaagca tgacgataca gagcagcggt agcaatgtgg tattgggtac aacgactgcc
   106381 gccataaaaa gacttaacca gccatcgcgt gttaccacca gcacgatgcc gagaatagcg
   106441 caggaaacgg ttattgccgc tggtacggcg ggaacgtgct catgcagcat caaccctaat
   106501 gccacgccga caaacactgc cgggaagatt cgcccaccgc gaaagccact ggcggcagca
   106561 acgaccaggg cggcaagttt aattaccgcc agcaaaaagt aatcgctggt gctgaaagcc
   106621 tgatttgcca ccatctgctg catctcatcc agccctttaa acagcgaaac tggtccacca
   106681 ataaccccca gaataccgag aataaatccg ccaataccca gcacgagcac cggatttttc
   106741 atttgatgca tcatcgcgtg caaccgtggt aagcaccata cggcgaccat ccctgctgcg
   106801 atggcaatcg ccgcgacaat tgcaccgctg agaatatcgg tcatttccat ctgtccgtaa
   106861 tgcgcaatgg gcagtgaaaa atggggatgg aaaaataatc cggtagtaag tgcaccagct
   106921 gctgccgcca ttaacggcgc aaaaagacga tcccatagcg gaacttcact actgccattt
   106981 aaggtttgcg aaaatatcaa cgccgccgca acaggtgtac caaacagcgc gccgatggtt
   107041 ccggcagagg ctaaaatagt ccactccatt cggttgacgc gcggtaacag acgagcgcca
   107101 atggcaaccg caagggcgat attgacggtc atgatcggat gttccggccc caggctgacg
   107161 ccgccagcaa gaccgagaat taatgctacg ataagtcctg gtagcgcaga gggcggaacc
   107221 ggtgcgccga tcagcggttc acaggcgggg tctggtccgg catgaccctg gctgaaacgg
   107281 ataaccaacc ccaccgcaat acccgttagc gttaatacac cgatgatcca gaggggtgaa
   107341 tcctgggcta tccccagagt tcccggcagt cgttgccaga gcaaattctg taataccgag
   107401 gcgattttca tcaccacaat cagaataaga ctggacgcaa tcccaattgc cacggcgggg
   107461 agcgataata acaacatggt tctggctcgc ggatggagca tgttctattc cttatgcggg
   107521 gtcagatact tagtttgccc agcttgcaaa aaggcatcgc tgcaattggt gctgaaacga
   107581 taaagtaatt gtgtgaccca gatcgatatt tacagggagc ctgcctttcc ggcgttgttg
   107641 ttatgccccc aggtatttac agtgtgagaa agaattattt tgactttagc ggagcagttg
   107701 aagaatgaca aagtatgcat tagtcggtga tgtgggcggc accaacgcac gtcttgctct
   107761 gtgtgatatt gccagtggtg aaatctcgca ggctaagacc tattcagggc ttgattaccc
   107821 cagcctcgaa gcggtcattc gcgtttatct tgaagaacat aaggtcgagg tgaaagacgg
   107881 ctgtattgcc atcgcttgcc cgattaccgg agactgggtg gcaatgacca accatacctg
   107941 ggcgttctca attgccgaaa tgaaaaagaa tctcggtttt agccatctgg aaattattaa
   108001 cgattttacc gctgtatcga tggcgatccc gatgctgaaa aaagagcatc tgattcagtt
   108061 tggtggcgca gaaccggtag aaggtaagcc tattgcggtt tacggtgccg gaacggggct
   108121 tggggttgcg catctggtcc atgtcgataa gcgttgggta agcttgccgg gcgaaggcgg
   108181 tcacgttgat tttgcgccga atagtgaaga agaggccatt atcctcgaaa tattgcgtgc
   108241 ggaaattggt catgtttcgg cggaacgcgt gctttctggc ccaggactgg tgaatttgta
   108301 tcgcgcaatt gttaaagccg acaaccgcct gccagaaaat ctcaagccaa aagatattac
   108361 cgaacgcgcg ctggctgaca gctgcaccga ttgccgccgt gcgttgtcgc tgttttgcgt
   108421 cattatgggc cgttttggcg gcaatctggc gctcaatctc gggacatttg gcggcgtgtt
   108481 tattgcgggc ggtatcgtgc cgcgcttcct tgagttcttc aaagcctctg gtttccgtgc
   108541 cgcatttgaa gataaagggc gctttaaaga atatgtccat gatattccgg tgtatctcat
   108601 cgtccatgac aatccgggtc ttctcggttc cggtgcacat ttacgccaga ccttaggtca
   108661 cattctgtaa atccttcctg ttatatcggg aggtaactct cccgataatc ttttaaatca
   108721 tacagttaat tcaacttttc tttgtgtccc ctcacaaggc cgacctgcgt cacacttccg
   108781 tacagcggga ttaattctcc agtaaatgca ttatttgtct ggtaacggcg atttgttttg
   108841 cacgttcata atttcactcg tcaactatca ctgaacgagg aattaacgat gagtaagaaa
   108901 ctgattgcct tatgtgcctg cccaatgggc ctggctcaca cttttatggc cgctcaggcg
   108961 ctggaagaag cggcggtaga agccggttat gaagtgaaaa ttgaaaccca gggcgcggac
   109021 ggtatccaga atcgcctgac ggcgcaggat atcgccgaag cgaccatcat catccactcc
   109081 gtggcagtta ccccggaaga taacgaacgt ttcgaatcac gcgacgttta tgaaatcact
   109141 ttgcaggacg caattaaaaa cgctgcgggc atcatcaaag aaatcgaaga gatgattgcc
   109201 tctgaacaac agtaatattc gacagggata attgttatgg ccattaaaaa acgcagtgca
   109261 accgttgtgc ctggcgcatc cggtgcggca gcggcagtta agaatccgca ggcctctaaa
   109321 agcagcttct ggggtgaact cccgcagcat gtgatgtcag ggatttcacg catggtgccg
   109381 accttaatta tgggcggtgt gatcctcgct ttcagccagt tgattgctta tagctggctt
   109441 aaaattcctg ctgagatcgg catcatggat gctcttaata gcgggaaatt ctccggtttc
   109501 gacctctcct tactgaaatt tgcctggttg tcgcagtcct tcggcggcgt gttgtttggt
   109561 tttgccattc cgatgtttgc tgcttttgtg gcgaactcta tcggtggcaa actggcgttt
   109621 ccggctggtt ttatcggcgg cttgatgtct acccagccga cgcaactgct gaacttcgat
   109681 cccagcacta tgcaatgggc gacctcttcg ccggtgccgt ccaccttcat tggtgcgctg
   109741 attatttcta tcgttgccgg ttaccttgtg aaatggatga accagaaaat ccagttaccg
   109801 gatttcctgt tagcgttcaa aaccacattt ttgctaccga ttctttccgc tatttttgtc
   109861 atgctggcga tgtactacgt catcaccccc tttgggggct ggatcaacgg cggtatccgt
   109921 actgtgctga ctgccgcagg tgagaagggc gcgctgatgt acgcaatggg gatcgccgcg
   109981 gcgacagcaa tcgaccttgg tggcccgatc aacaaagccg caggttttgt tgccttcagc
   110041 tttaccactg accacgtatt accggtcacc gctcgttcta tcgctatcgt tattccgccg
   110101 attggtctgg gtctggcgac cattattgac cgtcgtttaa ccggcaaacg cctgttcaac
   110161 gcacaacttt atccgcaggg taagaccgcg atgttccttg cctttatggg gatcagtgag
   110221 ggtgcgattc cgtttgcgct ggaaagcccc atcaccgcca ttccgtcgta tatggtcggc
   110281 gcgattgtcg gctcaaccgc cgctgtctgg ctgggcgcag tgcaatggtt cccggaatcc
   110341 gctatctggg catggccgct ggtcactaac ctgggcgtct atatggcggg gatcgcgctg
   110401 ggggcagtca taactgcgct gatggtggtg ttcctgcgtc tgatgatgtt ccgtaaaggc
   110461 aaattgttaa tcgatagcct gtaagaagga aaatccagat gacattactc gcttcgctgc
   110521 gcgactggct taaggcgcaa caactggatg cggtgcttct ctcctcacgg cagaacaaac
   110581 agccgcatct ggggatctcc accggatcag gctatgtgct gattagccgt gaaagtgcgc
   110641 acattctggt ggactcgcgc tattacgcgg atgtagaagc ccgcacgcaa ggttaccagc
   110701 tgcatttgct tgacgcgacg cacacgctta caaccatcgc caggcaaatc attgccgatg
   110761 agcagttgca aacgctcggt tttgaaggcc agcaggtgag ttgggaaacc gcgcatcgct
   110821 ggcagtctga actcaatgcg aaactggtta gcgccacgcc ggatgtgctg cggcaaatca
   110881 aaacgccaga ggaggtggag aaaatccgcc ttgcctgtgg gattgccgat cgcggtgcag
   110941 agcatattcg ccgctttatt caggcgggga tgagcgagcg cgagatagcc gctgaactgg
   111001 agtggtttat gcgccagcag ggcgcagaaa aaacctcttt tgacaccatt gtcgccagtg
   111061 gctggcgtgg ggcgctgccg cacggcaaag ccagcgacaa gattgttgca gcgggcgagt
   111121 ttgtcactct cgatttcggt gcgctctatc agggctactg ctctgatatg acgcgcacct
   111181 tgctggtgaa tggcgaaggg gtgagcgccg aatctcaccc gctgtttaac gtctatcaga
   111241 ttgttttgca ggcacagctc gcagcaatct ctgcaattcg ccccggcgtg cgctgccagc
   111301 aggttgacga agccgcgcgt cgggtgatta ccgaggcagg ttttagccac tatttcggtc
   111361 ataacaccgc tcatgctatc ggcattgaag ttcatgaaga tccgcgtttt tcaccgcggg
   111421 acaccacgac gctacagcca ggcatgttac tgaccgtgga gccggggatt tatttgccag
   111481 ggcaaggggg cgtgcgcatc gaggatgttg tgctggtcac cccgcaaggc gcagaagtgc
   111541 tctacgccat gccgaaaaca gtgttgctca cgggagaggc ataatggatt tatcgctatt
   111601 gaaagcgttg agcgaggcag atgcgatcgc ctcctcggaa caggaagtgc ggcagatcct
   111661 gctggaagaa gcggatcgcc tgcaaaaaga agtgcgattt gatgggctgg gatcggtgct
   111721 gatccgcctc aatgaatcga caggtccgaa ggtgatgatc tgtgcgcata tggacgaagt
   111781 gggatttatg gtgcgcagca tctcccgcga aggggcgatt gatgtgctgc cggttggcaa
   111841 tgtacgcatg gctgcccgcc agctgcagcc ggtgcgcatc accacccgtg aagagtgcaa
   111901 aattccaggc ctgcttgacg gcgaccggca ggggaatgat gtcagcgcca tgcgcgtgga
   111961 tattggcgcg cgctcgtatg acgaagtgat gcaggcggga attcgtccag gcgatcgcgt
   112021 cacgttcgat accacttttc aggttctccc ccaccagcgg gtgatgggga aagcctttga
   112081 tgaccgcctc ggttgctacc tgctggtgac gttactgcgc gaactacaca gcgctgaact
   112141 gcctgcggaa gtgtggctgg tggccagttc cagcgaagag gtggggttac gcggcgggca
   112201 aactgccacc cgcgcggtgt cgccggacgt cgccattgtc cttgataccg cctgctgggc
   112261 gaaaaacttt aattatggcg cggctaacca tcgccagatt ggtaacggcc cgatgctggt
   112321 gttaagcgac aagtcactga ttgcgccgcc aaaactcacc gcctggatcg aaaccgtggc
   112381 ggcagaaatt ggcgtgccgt tacaggcgga tatgttcagt aacggcggca cggacggtgg
   112441 agcggtgcac ttaaccggta ctggcgtacc cacagtggtg atggggcctg ccacccgcca
   112501 cggacattgc gccgcgtcga ttgccgattg ccgtgacatt ttgcagatgg agcaactttt
   112561 atctgccctt attcaacgtc ttacgcgtga gacggttgtt caactgacgg atttcagatg
   112621 atctcctgat taacccggag cggttatgtt aacgattcaa tttctctgtc ctttgcctaa
   112681 cggtctacat gctcgtccgg cgtgggaact taaagaacag tgcagccagt ggcaaagcga
   112741 aatcactttt attaaccatc gccagaacgc aaaggccgat gccaaaagct cgctggcgct
   112801 gattggcacc ggcaccctat ttaatgacag ttgcagcctg aacattagcg gcagcgatga
   112861 agagcaggcg cggcgcgtgc tggaagagta catccaggtg cgctttatcg acagcgacag
   112921 cgttcagcct acgcaggcag aactgacggc gcatccgctg ccgcgttcat taagccgcct
   112981 taacccggat ttactgtacg gcaatgtgct ggcaagcggc gtcggcgtgg gtacgctgac
   113041 cctgttacaa agcgacagcc tcgacagtta tcgggcaatc cccgccagtg cgcaagattc
   113101 cacccggctg gagcacagcc tggcaacgct tgccgagcaa ctgaaccagc aattgcgtga
   113161 gcgtgacggc gaaagcaaaa ctatcctcag cgcccatttg tcgctgattc aggatgatga
   113221 atttgcaggc aatatccgtc gcctgatgac agaacagcat caggggctgg gggcggcgat
   113281 catcagaaat atggagcagg tttgcgccaa actttctgct tctgccagcg attatctgcg
   113341 cgaacgtgtt agcgacattc gcgatattag cgaacagttg ctgcatatta cctagccgga
   113401 actgaagccg cgcaacaatc tggtgctgga aaaaccgact attctggtgg ccgaggattt
   113461 aaccccaagc cagtttttaa gcctggattt aaaaaatctt gcgggcatga ttctggagaa
   113521 aaccgggcgc acctcgcata ccctgattct ggcccgcgcc tcggcgatcc cggtactgag
   113581 cggcctgccg ctggatgcga tcgcgcgtta tgccgggcaa cctgcagtgc ttgacgccca
   113641 gtgcggcgtg ctggcgatta acccgaatga cgcggtgagc ggttattatc aggtcgcgca
   113701 gacgctggcg gataaacgcc aaaaacaaca ggcgcaggct gccgcgcagc tggcttattc
   113761 ccgtgataac aagcgtattg atatcgcggc gaatattggc accgctctgg aagcgcctgg
   113821 cgcgtttgcc aacggcgcgg aaggcgtcgg gctgttccgt accgaaatgc tctatatgga
   113881 tcgcgacagc gagccggacg agcaggagca atttgaagcc taccagcagg tgctactggc
   113941 agcgggcgac aagccgatta tcttccgcac aatggacatc ggaggcgata aaagcattcc
   114001 ttatctgaat attccccagg aagagaaccc gttcctcggc tatcgcgcgg tacgtattta
   114061 cccggaattt gctggcctgt tccgcactca actgcgggcc attttgcgcg ccgccagttt
   114121 cggcaacgcc cagttgatga tcccgatggt ccacggcctc gatcagatct tatgggtgaa
   114181 gggcgagatc caaaaagcga tcgttgagct taagcgcgat ggcctgcgtc atgcagagac
   114241 gattacgctg ggtatcatgg tggaagtgcc gtcggtgtgc tgcatcatcg atcacttctg
   114301 cgatgaggtc gatttcttca gtatcggctc caacgatatg acccagtatc tgtatgcggt
   114361 cgatcgtaat aacccgcgcg tatcgccgct atataacccg attacgccat cgttcctgcg
   114421 catgttgcag caaatagtta ccactgcgca tcagcggggc aaatgggtag gcatttgcgg
   114481 tgaactgggc ggtgaaagcc gttatctgcc gctactgctt gggctgggcc tggatgagct
   114541 gagtatgagt agcccgcgca ttccggcggt gaaaagccag cttcgtcaac tggatagcga
   114601 ggcgtgtcgg gaactggcac gtcaggcgtg tgaatgtcgc agcgcgcagg aaattgaagc
   114661 gttactcacc gcctttacgc cggaagaaga cgttcgcccg ctgctggcgc tggagaatat
   114721 cttcgttgat caggccttta gcaataaaga gcaggcgatc cagttcctgt gcggtaacct
   114781 cggcgttaac gggcgcactg aacatccgtt cgagctggaa gaggatgtct ggcagcggga
   114841 agagattgtc accactggcg ttggttttgg cgtggcgatc ccgcacacca aatctcagtg
   114901 gatccgtcat tccagtatca gcattgcccg gctggcgaaa ccggttgact ggcagtcaga
   114961 aatgggcgaa gtcgaactgg tgatcatgct gacgctgggt gctaacgaag ggatgaatca
   115021 tgtgaaagtc ttctcgcagc tggcgcgtaa actggtgaat aaaaacttcc gccagtcgtt
   115081 gtttgccgcg caagatgcac aaagtatcct gacgctgctg gaaacagaat taaccttctg
   115141 acgttagccc tgaaaacggg cgctgtactc tcccggcgtc agaccaaact gacgccggaa
   115201 aacgcgacaa aaatagtcgc tatccggaaa accgcaacgc tgtgccactt catgaattga
   115261 caaatgatat ttctgcaaaa tcatcctcgc cttcgccatt cgcacccaac gcacatactc
   115321 gataaaacgc atcgttccat gctgagcaaa cagttttgac agatgattgg gcgtgatatt
   115381 aaaaaactgc gccacgctct cgcgggtgag cggctgggca taattatcct gaacccagtt
   115441 acaaatgctg tgatagagaa aatcgccgcg cggctgcgaa ttgcccggcg gcatattcac
   115501 cacgttacgg caaagatgca gcaaacttaa caccagcggc tgaatgatat tttgctccag
   115561 cggcgaacgg ctaagatggg tgagggcggt aagcatcgct tcaccttcac cgcgttgcag
   115621 gctgggaagc tggatctggc gagcaggatg caacaatgac gtggtgcggc tatcgtaaaa
   115681 cgataaccca agccaggtcg gagcgaacac caggcttaac agcatcaccg gtttgttgtt
   115741 gaccggtaaa ttagccgcgc gcgccggaat aaacagcatt tctccttcca ccaaacgttg
   115801 ctgctttgct tccagcagat tgccgtattc gccgcgcagc acgatatcca gacggggaaa
   115861 atcaatacat aaactgccca ccggcaacga ggcaggctgg ctggcaaacc agacgcgccc
   115921 cagtagttgc gggttaagca ccaggccgct gaacagatcg gcgaaaaatt gttgatcggc
   115981 gggcaagcct ggtgctttca tgaaagttcc cgattaaaga tgcattaact ggcggaattc
   116041 tttaactttg ctgcggctga ccggcacttc aaaatccaga tctttcagcc gcagaatata
   116101 ggtgttatta aaccacggtt cgatttcgcg gattttgttc agattgacac aaaatgagcg
   116161 atggcagcgg aaaaaatgcg acggcggcag tttgctgcaa aattcggtaa tgttcatcgg
   116221 cattacgtag gattcacgcc gcgtataaac aaaggtcatt ttctcgtgcg cttcggcgta
   116281 atagatatcg ttaattggcg tgacgattat tcgctcatct ttcaccagat taatggtgtc
   116341 attctcacgc gttaccgtcg cggcaggcgt gctgctggtc tgctgttgtt gccaggctgc
   116401 ttccagtttt tgcagcatcc cggtaatgcg tgactcctga tacggtttta gaatgtagtc
   116461 aaacgcctcc agttcaaacg cttctaccgc atgttctttc cacgcggtga tgaacacaat
   116521 aaacggttta tgggcgaact ggctgatgtt ttgtgccagc aacacaccat ccagcgacgg
   116581 aatattgata tccagaaaaa tggcgtcgac gcggttatgc tgcaaaaact tcaacacgtc
   116641 cagaccgtcg tcaaaggtgc cgacaatctc catctggctg tgctctttaa ttagccagct
   116701 cagttcctgt tgtgccagga attcgtcttc aacaatgatg actttcacaa tatcactccg
   116761 gctcaaagca ataacgtagc ctgtgaggcg actggggtgc gttggttagg aatgtaaaac
   116821 gcaatttccg ttcctggttc caggcggcgg atatgcagcc cctcgccata caataacttc
   116881 acgcgatgat ggacattcag caggccaatt ttattgcccg gcatttcatt cgcttcgacc
   116941 cgctcaatca cctttggatc gatgccgtgc ccggtatccc gcaccgcaat gcgtacccga
   117001 ttgccgcact ctgcaacgct gatggtgaca acgcctttac ctttgcaagg ctggataccg
   117061 tggacaatgg cattctccac caacggctgg atcagcaggc tgggaatgca gcaattcacc
   117121 tcttcatcaa tatcatagat aaccgtcagc ttgtcgccaa agcgggcctg ctcaatggca
   117181 atatagtctt taatttgata cagctctttt ttgatatcga tttgctcatc gtcttttaat
   117241 tcaatgttat agcgcagata acgcgacaga ttaaagatca gctggcgagc ggtatccgga
   117301 ttcagacgga tcgacgatga aatagcgttc aaagcgttaa acaggaaatg gggattaatt
   117361 ttgctttgca gggcgcgaag ctctgcctta tttgccattt cacgtagctg ctcggcgcgt
   117421 gaaacctcca gttgcgtgga gatgatttgc gacagaccaa ccgccatttc ctgtaatgac
   117481 gaggttatct gatgcgcgtg gcagtagtaa attttcagcg ttccggtcac aacgcctttc
   117541 tcccacaatg ggatcaccag catggaatga atctctggtg tgcggtgggc ttcatcattg
   117601 tttttaatga tgatttttcc gtaattcatc gcctgacggg tagtcgggct aatgaagtca
   117661 tcgccattct gatagttatg ttcacccaca ccaacatagg ccagcacatg atcggtattg
   117721 gtaatcgcca ccgcatcggc gtgaatgtca tcgcgaataa tttcacaaac tttgcgtaat
   117781 gactcgctat tgacatggcg aaacagaggt agcgttttgt tggcgatatc cagcgccaac
   117841 ttggcctgcc gcgcggcgct ggcctctttt tcgccctcaa cgctttgcac tagaagcaca
   117901 ataaagccga tacagacgct gccgaggatc attggaatcc cgattttaga gacgatatcg
   117961 atccccaacg cggtagttgg tgcccagaca atcaccagaa tcatggtcaa cgtttcacac
   118021 aacatgccgc cgagaatacc gacgcgccag cgctgtgctt tggggatttt caggttgatc
   118081 cagcccgata tacaaccagc cagaatgctg gtgataaagc aggggatcgc cgtcacgcca
   118141 ccgatatcaa ttaaataacg atgaataccc gcaatcacgc cggtaatgat gcccacccac
   118201 gggccaaaca gaatcccgcc ggacatcacc gcgataatac gtacgttcac cagcgagcct
   118261 tctacgggaa cgccggacca ggtgctgaag agggcgaaca gcgagaaaat ggcggtaacg
   118321 gcgagcaact ctttcggtga gtgtgccgac ttgtgcaaca gttcgcgaaa caggcggata
   118381 cggatgagaa agaacaggca gataagcatt aacgctgccc gatcgaagac cgccagcagc
   118441 atgttgaata tttcgtgcac gggtgactct gaataaggat taaacctcta tgataggtaa
   118501 cctgaaggct gatgaccagc aggccgtttt tgaggaaaaa ccacaatttt aaaaggggat
   118561 tgatagataa atggtgtatt agcaatgcat ctcttgataa tgcacttctg gttagtaaat
   118621 ttattgaaat tattatgtta ataaaatgag tggtaatttt cttgttagcg cagataaatt
   118681 tcctgttttt aaagcagagt tcctcattta acgtcattaa taaaaatgtt ctaaaacgct
   118741 ggacagacag aaattaatca ggctattttc aaaacagcca ccatcgtggc agcgtcgctc
   118801 ggacggtccg ggcgctaacg ttaatctgag gaaattatgg ctgacactcg ccctgaacgt
   118861 cgctttacgc gcattgatcg tctcccgccc tacgttttta acatcaccgc tgaactgaaa
   118921 atggctgcgc gtcggcgcgg cgaagatatt atcgatttca gcatgggtaa cccggacggt
   118981 gcgactccgc cgcatatcgt cgaaaaatta tgtactgtgg cccagcgtcc ggacacgcat
   119041 ggttactcca cttcacgcgg cattccgcgg ttacgtcgcg ccatttcccg ctggtatcag
   119101 gatcgctacg acgttgaaat cgatccggaa tcagaagcca tcgtcaccat tggttcgaaa
   119161 gaagggctgg cacacctgat gctggcgacg ctggatcatg gtgacacggt gctggtgccg
   119221 aatccaagct acccgatcca tatttatggc gcggtgattg ccggggcgca ggtacgctca
   119281 gtgccgctgg tggaaggtgt cgatttcttc aacgaactgg agcgcgccat tcttgaaagt
   119341 tatccgaaac cgaagatgat gatcctcggc ttcccgtcaa acccaaccgc gcaatgcgtg
   119401 gaactggagt tcttcgaaaa agtagtggcg ctggcgaaac gctacgatgt gctggtggtc
   119461 catgacctgg cctatgccga tatcgtctac gatggctgga aagcgccgtc aatcatgcag
   119521 gtaccgggtg cacgcgatgt ggcagtcgag ttctttacgc tgtcgaaaag ctacaacatg
   119581 gcgggctggc gtatcggctt tatggtcggc aacaaaacgc tggtcagtgc cctggcgcga
   119641 atcaaaagct atcacgatta cggcactttt acgccgttgc aggtggcggc gattgcggcg
   119701 ctggagggcg atcaacagtg cgtgcgcgac attgccgaac agtacaaacg ccgccgcgat
   119761 gtattggtta aagggctgca tgaagcgggc tggatggtcg aaatgccgaa ggcttcgatg
   119821 tatgtctggg cgaaaatccc ggaaccatat gcggccatgg gatcgctgga atttgccaag
   119881 aagctcctta acgaagcgaa ggtctgtgtc tctccaggga ttggctttgg tgactacggc
   119941 gacacgcatg ttcgctttgc actgattgaa aaccgcgacc gtattcgtca ggcgattcgt
   120001 gggattaaag cgatgttccg tgccgacggt ttactacccg ccagcagcaa acatattcac
   120061 gaaaacgcgg aataaacacc gtcgtcagaa atgcaaacag gagcctgatg gctcctgttt
   120121 ttcattgcac agtttgcttt tagatcatca gggcaaaagt accggcccaa acgatgacca
   120181 taaaagaaat gcccataaag aaatatttca ctttacatct ctccgcgtaa ttacggttac
   120241 gcctgaatga cttcatcaca cctgatacag tgagttgaca ccgggcaaaa cgaccttaaa
   120301 agtcctgtat tttaccgtgg gcatcacccc agaattctga gctaaactgc tcgagacggc
   120361 gctaatgtac gcctaaaact gaaacgagac aagaggcatt ttcttattta cttttagtaa
   120421 cttacaggtg tctcatatcg gcgacagttt aatttctggc ggtaataatt agatattcag
   120481 cgaaggaaaa aatcttaaga aaaaagccat aaaaaccatg aggttattat ggccgatttg
   120541 aggagggaaa gagtaagagc agtttgttaa atgtacaacg acgattctcc caccgggcgc
   120601 gttttaaagc gacggtggat ccagaggtac tgctccggtg cgcgcatgat ctctttctcg
   120661 ataatcttgt tcatataggc agcggcttga ttttcatctg tcgggtagcc ttccatctct
   120721 ggggtgatga acaaacgata tccgctgtaa tccgcttttc ttaccatcgt tacggtcaac
   120781 atggctgcgc cagagagacg ggagagaaca taggtgccat tggttgtggc gacattttcc
   120841 accgcaaaga acggcgcgaa ggagctgcct ttacgaccat aatcctgatc gggagcaaac
   120901 cataccgctt cacctttctt cagtgcaccg acaatgccgc gcagattatt tctgccgatc
   120961 atcgctttgt tagagcgcat gcgcccacgg gtctgcaccc attccatcag ctgattatta
   121021 tgtggacgat aggtagccat cattggctga caaagtccca tcacgcggcc gcccagttcc
   121081 agcgacataa aatggacgcc gacaaccatt acgccgcgat tttgcatttg tgcgcgttta
   121141 aggttatcca gcccttcaac atcaaaccat ttacgtacgc gactgtcggg ccagaaccac
   121201 gccatgccgg tttctaccag agccatgccg agtgaacgaa agttttcggc aatcatcttc
   121261 tcgcgttctt ccgcagaatg ctgcgggaag caaagttcca ggtttttacg ggcgatagat
   121321 tcacgccgtt tcaggaatgg tcgcgccatt gcgccaatac gcgtgccgag aaagcagaga
   121381 acaggataag gaagctgtac ccaaagccag agtacaccaa gcccaaacca tgtgagccag
   121441 tagcgaggat gtagaaactc gcgggaaaat ttgcattgtg gaaacatagt taccttctgt
   121501 agatacgaat tgttgtatcg cagaagattc catgtcgaca gcggtacact caattggttg
   121561 ggccatcatg tacgacaatg gttcctgtaa agagtgtaaa gactagtcag gacataacca
   121621 cgaggagcat gaatgcggta tctgtacttt atcactggct gactgctttc agctactgat
   121681 tattaatcgt gcaaagtaaa aaattttccg cacgcaatag tattgcattt aacatgctgt
   121741 tttcattgac gcttagcaaa aattgccaga ggttaaggat tgtcttacta ttaaagtgac
   121801 tgaagttaca ggttttttag cgcggcagga ggttgagcta tgttttattt atggatgttt
   121861 ctcgccctgt gtattgtctg cgtaagcgga tacatcggtc aggtactgaa cgtggtatcg
   121921 gcggtctctt ctttcttcgg catggtgatc ctcgctgcac tcatttatta cttcaccatg
   121981 tggttaactg gcggtaatga actggtgacg gggatattta tgtttcttgc cccggcttgt
   122041 ggcttgatga ttcgctttat ggtggggtat ggcaggagat agtgggtcag gatggttcat
   122101 ttgggcaggt gttcacatca gcatcagaca tgattgttca cctgccaggt gacagacgct
   122161 atcgcacagg atgaataaga taattcgcct ttgtcggaaa agggggcgtg gtttcttctg
   122221 tgaggtttaa tggtggtgca tcttctctca tcttaaacaa aatcgcctgt gatggctgaa
   122281 attttgtaga gtgagttgtc gtcatcgctg acgttttttc tggctcttta tagctcgtct
   122341 cactaccacc accagaatta gaaaatgcaa aaacattgca tgccataata gagaccaata
   122401 aaaataagct aaaaagtatt aagtttactt tcatagaaag ttatcctgaa aatggttcac
   122461 tgagaatgta cgatgtt
//
LOCUS       DAZFAB010000002       107485 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZFAB010000002 DAZFAB010000000
VERSION     DAZFAB010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 107485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 107485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..107485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.002"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            133..1246
                     /gene="ompN"
                     /locus_tag="ACHJWA_000120"
                     /pseudo
     CDS             133..1246
                     /gene="ompN"
                     /locus_tag="ACHJWA_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            1387..1821
                     /gene="uspF"
                     /locus_tag="ACHJWA_000121"
     CDS             1387..1821
                     /gene="uspF"
                     /locus_tag="ACHJWA_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFO4306807.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            1997..2932
                     /gene="ttcA"
                     /locus_tag="ACHJWA_000122"
     CDS             1997..2932
                     /gene="ttcA"
                     /locus_tag="ACHJWA_000122"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFO4306808.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(3061..4434)
                     /gene="dbpA"
                     /locus_tag="ACHJWA_000123"
     CDS             complement(3061..4434)
                     /gene="dbpA"
                     /locus_tag="ACHJWA_000123"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFO4306809.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(4467..4637)
                     /gene="ynaL"
                     /locus_tag="ACHJWA_000124"
     CDS             complement(4467..4637)
                     /gene="ynaL"
                     /locus_tag="ACHJWA_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFO4306810.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRA"
     gene            complement(4912..5895)
                     /gene="zntB"
                     /locus_tag="ACHJWA_000125"
     CDS             complement(4912..5895)
                     /gene="zntB"
                     /locus_tag="ACHJWA_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFO4306811.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(5909..5956)
                     /gene="ydaN"
                     /locus_tag="ACHJWA_000126"
     CDS             complement(5909..5956)
                     /gene="ydaN"
                     /locus_tag="ACHJWA_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFO4306812.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            6150..7382
                     /gene="dgcM"
                     /locus_tag="ACHJWA_000127"
     CDS             6150..7382
                     /gene="dgcM"
                     /locus_tag="ACHJWA_000127"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFO4306813.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFIHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(7403..7966)
                     /gene="smrA"
                     /locus_tag="ACHJWA_000128"
     CDS             complement(7403..7966)
                     /gene="smrA"
                     /locus_tag="ACHJWA_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFO4306814.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(8296..9204)
                     /gene="abgR"
                     /locus_tag="ACHJWA_000129"
     CDS             complement(8296..9204)
                     /gene="abgR"
                     /locus_tag="ACHJWA_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4306815.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            9380..10690
                     /gene="abgA"
                     /locus_tag="ACHJWA_000130"
     CDS             9380..10690
                     /gene="abgA"
                     /locus_tag="ACHJWA_000130"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HFO4306816.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNENSRMGLPDELTLQREFERARQQGALAQWIAAFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTTIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGYNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            10690..12135
                     /gene="abgB"
                     /locus_tag="ACHJWA_000131"
     CDS             10690..12135
                     /gene="abgB"
                     /locus_tag="ACHJWA_000131"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HFO4306817.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSVTP
                     GENGHGCGHNLLGTAAFAAAIAIKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYQALSHFGTPEWNSEELAFAKQIQ
                     ATLTSNDRQNSLNNIAATGGENGKVFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTLNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            12172..13698
                     /gene="abgT"
                     /locus_tag="ACHJWA_000132"
     CDS             12172..13698
                     /gene="abgT"
                     /locus_tag="ACHJWA_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HFO4306818.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            13709..14224
                     /gene="ogt"
                     /locus_tag="ACHJWA_000133"
     CDS             13709..14224
                     /gene="ogt"
                     /locus_tag="ACHJWA_000133"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFO4306819.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGELAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            14419..15171
                     /gene="fnr"
                     /locus_tag="ACHJWA_000134"
     CDS             14419..15171
                     /gene="fnr"
                     /locus_tag="ACHJWA_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFO4306820.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            15323..16273
                     /gene="uspE"
                     /locus_tag="ACHJWA_000135"
     CDS             15323..16273
                     /gene="uspE"
                     /locus_tag="ACHJWA_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFO4306821.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(16323..16580)
                     /gene="ynaJ"
                     /locus_tag="ACHJWA_000136"
     CDS             complement(16323..16580)
                     /gene="ynaJ"
                     /locus_tag="ACHJWA_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFO4306822.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            16824..17855
                     /gene="ynaI"
                     /locus_tag="ACHJWA_000137"
     CDS             16824..17855
                     /gene="ynaI"
                     /locus_tag="ACHJWA_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFO4306823.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
     gene            complement(17906..19519)
                     /gene="mppA"
                     /locus_tag="ACHJWA_000138"
     CDS             complement(17906..19519)
                     /gene="mppA"
                     /locus_tag="ACHJWA_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFO4306824.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDVHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(19855..20754)
                     /gene="pgrR"
                     /locus_tag="ACHJWA_000139"
     CDS             complement(19855..20754)
                     /gene="pgrR"
                     /locus_tag="ACHJWA_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFO4306825.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLIIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            20892..21812
                     /locus_tag="ACHJWA_000140"
     CDS             20892..21812
                     /locus_tag="ACHJWA_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO4306826.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAIMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPTQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            21812..22742
                     /locus_tag="ACHJWA_000141"
                     /pseudo
     CDS             21812..22742
                     /locus_tag="ACHJWA_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622627.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            22763..23401
                     /locus_tag="ACHJWA_000142"
     CDS             22763..23401
                     /locus_tag="ACHJWA_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309933.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO4306827.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSPKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            23692..24420
                     /gene="mpaA"
                     /locus_tag="ACHJWA_000143"
     CDS             23692..24420
                     /gene="mpaA"
                     /locus_tag="ACHJWA_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFO4306828.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSLVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(24395..25360)
                     /gene="ycjG"
                     /locus_tag="ACHJWA_000144"
     CDS             complement(24395..25360)
                     /gene="ycjG"
                     /locus_tag="ACHJWA_000144"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFO4306829.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDTTLIVDANESWHAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            25479..25985
                     /gene="tpx"
                     /locus_tag="ACHJWA_000145"
     CDS             25479..25985
                     /gene="tpx"
                     /locus_tag="ACHJWA_000145"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFO4306830.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(26029..27570)
                     /gene="tyrR"
                     /locus_tag="ACHJWA_000146"
     CDS             complement(26029..27570)
                     /gene="tyrR"
                     /locus_tag="ACHJWA_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFO4306831.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRASKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(27718..28779)
                     /gene="ycjF"
                     /locus_tag="ACHJWA_000147"
     CDS             complement(27718..28779)
                     /gene="ycjF"
                     /locus_tag="ACHJWA_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFO4306832.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(28776..30173)
                     /gene="ycjX"
                     /locus_tag="ACHJWA_000148"
     CDS             complement(28776..30173)
                     /gene="ycjX"
                     /locus_tag="ACHJWA_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFO4306833.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRYKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            30328..31326
                     /gene="ycjW"
                     /locus_tag="ACHJWA_000149"
     CDS             30328..31326
                     /gene="ycjW"
                     /locus_tag="ACHJWA_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO4306834.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHTPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLEDHNIAVRD
                     EWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGRQSCELLFRLIAGKPSPQNITVATHMTLK"
     gene            complement(31437..32342)
                     /gene="ompG"
                     /locus_tag="ACHJWA_000150"
     CDS             complement(31437..32342)
                     /gene="ompG"
                     /locus_tag="ACHJWA_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFO4306835.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRISLAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSISLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(32387..33469)
                     /locus_tag="ACHJWA_000151"
     CDS             complement(32387..33469)
                     /locus_tag="ACHJWA_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO4306836.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQESLH
                     KPIVMGMRPEDIHPDAQEENNISAKISVVELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            complement(33483..34142)
                     /gene="pgmB"
                     /locus_tag="ACHJWA_000152"
     CDS             complement(33483..34142)
                     /gene="pgmB"
                     /locus_tag="ACHJWA_000152"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFO4306837.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERTQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWPRLSAFWQN
                     V"
     gene            complement(34139..36406)
                     /gene="ycjT"
                     /locus_tag="ACHJWA_000153"
     CDS             complement(34139..36406)
                     /gene="ycjT"
                     /locus_tag="ACHJWA_000153"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFO4306838.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSHEAWQRELDFASGELR
                     RNVVWRTSNGAGYTIASRRFVSADQLPLIALEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEICAQQSYDQLLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRSGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMARYNVQQALNIARQFGCSDDA
                     FIHRAEMFLKELWMPETQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAVSCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(36403..37458)
                     /gene="ycjS"
                     /locus_tag="ACHJWA_000154"
     CDS             complement(36403..37458)
                     /gene="ycjS"
                     /locus_tag="ACHJWA_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO4306839.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKQGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGYPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQREMADDNRHLGSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(37468..38256)
                     /gene="ycjR"
                     /locus_tag="ACHJWA_000155"
     CDS             complement(37468..38256)
                     /gene="ycjR"
                     /locus_tag="ACHJWA_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFO4306840.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNIEEV
                     KSAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYSVENNLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLTWLRTC"
     gene            complement(38274..39326)
                     /gene="ycjQ"
                     /locus_tag="ACHJWA_000156"
     CDS             complement(38274..39326)
                     /gene="ycjQ"
                     /locus_tag="ACHJWA_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFO4306841.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETIIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWKLLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(39357..40199)
                     /gene="ycjP"
                     /locus_tag="ACHJWA_000157"
     CDS             complement(39357..40199)
                     /gene="ycjP"
                     /locus_tag="ACHJWA_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO4306842.1"
                     /translation="MATNKRTLSRIGFYRGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPVIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(40186..41067)
                     /gene="ycjO"
                     /locus_tag="ACHJWA_000158"
     CDS             complement(40186..41067)
                     /gene="ycjO"
                     /locus_tag="ACHJWA_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO4306843.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVIFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(41088..42380)
                     /gene="ycjN"
                     /locus_tag="ACHJWA_000159"
                     /pseudo
     CDS             complement(41088..42380)
                     /gene="ycjN"
                     /locus_tag="ACHJWA_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(42394..44072)
                     /gene="ycjM"
                     /locus_tag="ACHJWA_000160"
                     /pseudo
     CDS             complement(42394..44072)
                     /gene="ycjM"
                     /locus_tag="ACHJWA_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
     gene            complement(44282..44596)
                     /gene="pspE"
                     /locus_tag="ACHJWA_000161"
     CDS             complement(44282..44596)
                     /gene="pspE"
                     /locus_tag="ACHJWA_000161"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFO4306844.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATVVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(44671..44892)
                     /gene="pspD"
                     /locus_tag="ACHJWA_000162"
     CDS             complement(44671..44892)
                     /gene="pspD"
                     /locus_tag="ACHJWA_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFO4306845.1"
                     /translation="MNTRWQQAEQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(44901..45260)
                     /gene="pspC"
                     /locus_tag="ACHJWA_000163"
     CDS             complement(44901..45260)
                     /gene="pspC"
                     /locus_tag="ACHJWA_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFO4306846.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(45260..45484)
                     /gene="pspB"
                     /locus_tag="ACHJWA_000164"
     CDS             complement(45260..45484)
                     /gene="pspB"
                     /locus_tag="ACHJWA_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFO4306847.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(45538..46206)
                     /gene="pspA"
                     /locus_tag="ACHJWA_000165"
     CDS             complement(45538..46206)
                     /gene="pspA"
                     /locus_tag="ACHJWA_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFO4306848.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            46373..47350
                     /gene="pspF"
                     /locus_tag="ACHJWA_000166"
     CDS             46373..47350
                     /gene="pspF"
                     /locus_tag="ACHJWA_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFO4306849.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(47481..48746)
                     /gene="puuE"
                     /locus_tag="ACHJWA_000167"
     CDS             complement(47481..48746)
                     /gene="puuE"
                     /locus_tag="ACHJWA_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HFO4306850.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERAMQLGQRLTKTL
                     IDAKENVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALRD"
     gene            complement(48784..50064)
                     /gene="puuB"
                     /locus_tag="ACHJWA_000168"
     CDS             complement(48784..50064)
                     /gene="puuB"
                     /locus_tag="ACHJWA_000168"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFO4306851.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLEMLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTATGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSDDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYDARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(50066..51553)
                     /gene="puuC"
                     /locus_tag="ACHJWA_000169"
     CDS             complement(50066..51553)
                     /gene="puuC"
                     /locus_tag="ACHJWA_000169"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFO4306852.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLANIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLIEANAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVVLKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQQAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIQEGESKGQLLLDGRNAGLAVAIGPTIIVDVDPNAS
                     LSREEIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(51703..52260)
                     /gene="puuR"
                     /locus_tag="ACHJWA_000170"
     CDS             complement(51703..52260)
                     /gene="puuR"
                     /locus_tag="ACHJWA_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFO4306853.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIILTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(52287..53051)
                     /gene="puuD"
                     /locus_tag="ACHJWA_000171"
     CDS             complement(52287..53051)
                     /gene="puuD"
                     /locus_tag="ACHJWA_000171"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFO4306854.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPERDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            53263..54681
                     /gene="puuA"
                     /locus_tag="ACHJWA_000172"
     CDS             53263..54681
                     /gene="puuA"
                     /locus_tag="ACHJWA_000172"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFO4306855.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            54830..54994
                     /locus_tag="ACHJWA_000173"
     CDS             54830..54994
                     /locus_tag="ACHJWA_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4306856.1"
                     /translation="MPMIKSPHGAGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            54984..56366
                     /gene="puuP"
                     /locus_tag="ACHJWA_000174"
     CDS             54984..56366
                     /gene="puuP"
                     /locus_tag="ACHJWA_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFO4306857.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFAAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVAMSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRKGMNK
                     SWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPLY
                     EGDRTPVSET"
     gene            56500..56745
                     /gene="ymjA"
                     /locus_tag="ACHJWA_000175"
     CDS             56500..56745
                     /gene="ymjA"
                     /locus_tag="ACHJWA_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2543 family protein"
                     /protein_id="HFO4306858.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            57058..58701
                     /gene="sapA"
                     /locus_tag="ACHJWA_000176"
     CDS             57058..58701
                     /gene="sapA"
                     /locus_tag="ACHJWA_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFO4306859.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTAWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKLLGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKTLSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            58698..59663
                     /gene="sapB"
                     /locus_tag="ACHJWA_000177"
     CDS             58698..59663
                     /gene="sapB"
                     /locus_tag="ACHJWA_000177"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFO4306860.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            59650..60540
                     /gene="sapC"
                     /locus_tag="ACHJWA_000178"
     CDS             59650..60540
                     /gene="sapC"
                     /locus_tag="ACHJWA_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFO4306861.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCM
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            60540..61532
                     /gene="sapD"
                     /locus_tag="ACHJWA_000179"
     CDS             60540..61532
                     /gene="sapD"
                     /locus_tag="ACHJWA_000179"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFO4306862.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            61534..62340
                     /gene="sapF"
                     /locus_tag="ACHJWA_000180"
     CDS             61534..62340
                     /gene="sapF"
                     /locus_tag="ACHJWA_000180"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFO4306863.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTNLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(62392..63555)
                     /locus_tag="ACHJWA_000181"
     CDS             complement(62392..63555)
                     /locus_tag="ACHJWA_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HFO4306864.1"
                     /translation="MARVSLSWALILGLLAGIGPMCTDLYLPALPEMSEQLAATTTIT
                     QLTLTASLIGLGVGQLLFGPLSDKIGRKRPLILSLLLFIVSSILCATTNNIYWLVVWR
                     FIQGIAGAGGSVLSRSIARDKYQGVTLTQFFALLMTVNGLAPVLSPVLGGYIVSTFDW
                     RTLFWVMAEISTVLLLGCVLFINETLPENKRGSSLLLTGRSVVQNRRFMRFCLIQSFM
                     LAGLFAYIGSSSFVLQKEFGFSPMQFSLVFGLNGIGLIIASWIFSRLARRINAMTLLR
                     GGLIAAILCALLTVLCAWTQLPIPALVALFFTIAFCSGIGTVGGAEAMSAVGTQESGT
                     ASALMGMSMFVFGGIAAPLSGFGGETLLKMSLAITVCYTLAFLVALTRIDNQK"
     gene            complement(63564..64945)
                     /locus_tag="ACHJWA_000182"
                     /pseudo
     CDS             complement(63564..64945)
                     /locus_tag="ACHJWA_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309892.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
     gene            complement(64949..68057)
                     /locus_tag="ACHJWA_000183"
                     /pseudo
     CDS             complement(64949..68057)
                     /locus_tag="ACHJWA_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133655.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
     gene            complement(68057..69178)
                     /locus_tag="ACHJWA_000184"
     CDS             complement(68057..69178)
                     /locus_tag="ACHJWA_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFO4306865.1"
                     /translation="MKYIATSVVAMLLLSGCDNTQSNNSSPSETEVGVVTVKSQPVSV
                     VSELTGRTSAALSAEVRPQVGGIIQKRLFKEGDLVKAGQPLYQIDAASYQAAWNEARA
                     ALQQAQALVKADCQKAQRYARLVKENGVSQQDADDAQSTCAQDKASVAAKKAALETAR
                     INLDWTTVTAPISGRIGISSVTPGALVTASQDTALTTIRGLDTMYVDLTRSSVDLLRL
                     RKQSLATNSDTMSVSLILEDGTTYSEKGRLELTEVAVDESTGSVTLRAIFPNPQQQLL
                     PGMFVRARVDEGVMEDAILAPQQGVTRDAKGNATALVVNKDNKVEQRTLETGETYGDK
                     WLVLNGLHSGDRLIVEGSAKVTSGQTVKAVEVQANGGNA"
     gene            complement(69327..69893)
                     /locus_tag="ACHJWA_000185"
     CDS             complement(69327..69893)
                     /locus_tag="ACHJWA_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO4306866.1"
                     /translation="MTSKLEIRHKQRQDEIINAARRCFRRCGFHAASMSQIASEAQLS
                     VGQIYRYFANKDAIIEEMVRRIIDFRIAQMDIDARTDHFPEVLALRKSLNEDDDALML
                     EVAAEATRNPRVMAMLEEADARMFANGCAHMKRMHPHLSEEHIRCCVEVFATMMEGTV
                     YRRLTPQKSDPQHLQEIYQDIVSMLINK"
     gene            70371..71159
                     /gene="fabI"
                     /locus_tag="ACHJWA_000186"
     CDS             70371..71159
                     /gene="fabI"
                     /locus_tag="ACHJWA_000186"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFO4306867.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            71303..72430
                     /gene="yciW"
                     /locus_tag="ACHJWA_000187"
     CDS             71303..72430
                     /gene="yciW"
                     /locus_tag="ACHJWA_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFO4306868.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LEKETLAPFLSWLDPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSTRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESLRWRSSYEV
                     EKLPEEHTKSSTAELCQLAEILSLHPISLSLLERLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFAACMDSSNEYKKISPLLRKGENEINQWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            72498..74432
                     /gene="rnb"
                     /locus_tag="ACHJWA_000188"
     CDS             72498..74432
                     /gene="rnb"
                     /locus_tag="ACHJWA_000188"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFO4306869.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVRKDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(74551..74613)
                     /locus_tag="ACHJWA_000189"
                     /pseudo
     CDS             complement(74551..74613)
                     /locus_tag="ACHJWA_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            74667..76652
                     /gene="pdeR"
                     /locus_tag="ACHJWA_000190"
     CDS             74667..76652
                     /gene="pdeR"
                     /locus_tag="ACHJWA_000190"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO4306870.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPERTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLNEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTRKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQELIDHAINQADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSAAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTAY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            complement(76634..76762)
                     /gene="ymiD"
                     /locus_tag="ACHJWA_000191"
     CDS             complement(76634..76762)
                     /gene="ymiD"
                     /locus_tag="ACHJWA_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFO4306871.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTEYSAKYIYSSTYARFR"
     gene            76800..76973
                     /gene="yciZ"
                     /locus_tag="ACHJWA_000192"
     CDS             76800..76973
                     /gene="yciZ"
                     /locus_tag="ACHJWA_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFO4306872.1"
                     /translation="MSEFDAQRVAERIDIVLDILVADDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            77063..77812
                     /gene="yciT"
                     /locus_tag="ACHJWA_000193"
     CDS             77063..77812
                     /gene="yciT"
                     /locus_tag="ACHJWA_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFO4306873.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            78081..78299
                     /gene="osmB"
                     /locus_tag="ACHJWA_000194"
     CDS             78081..78299
                     /gene="osmB"
                     /locus_tag="ACHJWA_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFO4306874.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(78425..78751)
                     /gene="yciH"
                     /locus_tag="ACHJWA_000195"
     CDS             complement(78425..78751)
                     /gene="yciH"
                     /locus_tag="ACHJWA_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFO4306875.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRSKGDGVVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(78751..79488)
                     /gene="pyrF"
                     /locus_tag="ACHJWA_000196"
     CDS             complement(78751..79488)
                     /gene="pyrF"
                     /locus_tag="ACHJWA_000196"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFO4306876.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDAALAFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(79681..80850)
                     /gene="lapB"
                     /locus_tag="ACHJWA_000197"
     CDS             complement(79681..80850)
                     /gene="lapB"
                     /locus_tag="ACHJWA_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFO4306877.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(80857..81165)
                     /gene="lapA"
                     /locus_tag="ACHJWA_000198"
     CDS             complement(80857..81165)
                     /gene="lapA"
                     /locus_tag="ACHJWA_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFO4306878.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLVRAERKIKRLENQLSPATDVAVVQHSSAAKE
                     "
     gene            complement(81314..82078)
                     /gene="pgpB"
                     /locus_tag="ACHJWA_000199"
     CDS             complement(81314..82078)
                     /gene="pgpB"
                     /locus_tag="ACHJWA_000199"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFO4306879.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            82248..82838
                     /gene="ribA"
                     /locus_tag="ACHJWA_000200"
     CDS             82248..82838
                     /gene="ribA"
                     /locus_tag="ACHJWA_000200"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFO4306880.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(82902..85577)
                     /gene="acnA"
                     /locus_tag="ACHJWA_000201"
     CDS             complement(82902..85577)
                     /gene="acnA"
                     /locus_tag="ACHJWA_000201"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFO4306881.1"
                     /translation="MSSTLREASKDALQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLDLDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPIYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VARSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(85741..85836)
                     /gene="ymiC"
                     /locus_tag="ACHJWA_000202"
     CDS             complement(85741..85836)
                     /gene="ymiC"
                     /locus_tag="ACHJWA_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFO4306882.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(85950..86117)
                     /gene="yciX"
                     /locus_tag="ACHJWA_000203"
     CDS             complement(85950..86117)
                     /gene="yciX"
                     /locus_tag="ACHJWA_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFO4306883.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(86120..86260)
                     /gene="ymiA"
                     /locus_tag="ACHJWA_000204"
     CDS             complement(86120..86260)
                     /gene="ymiA"
                     /locus_tag="ACHJWA_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFO4306884.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
     gene            complement(86579..87553)
                     /gene="cysB"
                     /locus_tag="ACHJWA_000205"
     CDS             complement(86579..87553)
                     /gene="cysB"
                     /locus_tag="ACHJWA_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFO4306885.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(87763..90360)
                     /gene="topA"
                     /locus_tag="ACHJWA_000206"
     CDS             complement(87763..90360)
                     /gene="topA"
                     /locus_tag="ACHJWA_000206"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFO4306886.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            90740..90991
                     /gene="yciN"
                     /locus_tag="ACHJWA_000207"
     CDS             90740..90991
                     /gene="yciN"
                     /locus_tag="ACHJWA_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFO4306887.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(91027..92076)
                     /gene="sohB"
                     /locus_tag="ACHJWA_000208"
     CDS             complement(91027..92076)
                     /gene="sohB"
                     /locus_tag="ACHJWA_000208"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFO4306888.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVSTDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            92296..93054
                     /gene="yciK"
                     /locus_tag="ACHJWA_000209"
     CDS             92296..93054
                     /gene="yciK"
                     /locus_tag="ACHJWA_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFO4306889.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCRSEDCQQLAQRIAVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVIQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            93051..93641
                     /gene="cobO"
                     /locus_tag="ACHJWA_000210"
     CDS             93051..93641
                     /gene="cobO"
                     /locus_tag="ACHJWA_000210"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFO4306890.1"
                     /translation="MSDERYQQRQQRVKEKVDARMAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDLVLLDELTYMVAYDYLPLEEVLQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPVKHAFDAGVKAQIGIDY"
     gene            complement(93681..94553)
                     /gene="rluB"
                     /locus_tag="ACHJWA_000211"
     CDS             complement(93681..94553)
                     /gene="rluB"
                     /locus_tag="ACHJWA_000211"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFO4306891.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(94766..96349)
                     /locus_tag="ACHJWA_000212"
     CDS             complement(94766..96349)
                     /locus_tag="ACHJWA_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HFO4306892.1"
                     /translation="MAGKFRCILLLIVGLFFSSLSYAKNTEMPSYEEGISLFDVEATL
                     QPDGVLDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVTRDGIPEP
                     WHLDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAFLREGDSDLLIWNVTGNHWPF
                     EIYKTRFSLKFPDIAGNPFSEIDLFTGEEGDTYRNGRILEDGRIESSDPFYREDFTVL
                     YRWPHSLLSNAPAPQTTNIFSHLLLPSTSSLLIWFPCLFLVCGWLYLWKRRPQFTSVD
                     VLRMQKFYLPRKKSSFYRPDTFLQWGGLAILAVILYGNLSPVGWAGMSLVGDMFIMIC
                     WLLPFLFCSLELLFARDDDKPCVNRVIITLFLPLICSGVAFYSLYINVGDVFFYWYMP
                     AGYFSAVFLTGYLTGMGYIFLPKFTQTGQQRYAHGEAIVNYLARKEAATHSGRRRKGE
                     TRKLDYALLGWAVSANLGREWVARITPSLTAAVRAPEIARSGVLFSLQMHLSLGANTS
                     LLGRSYSGGAAGGGGGGGW"
     gene            complement(96377..96997)
                     /gene="yciO"
                     /locus_tag="ACHJWA_000213"
     CDS             complement(96377..96997)
                     /gene="yciO"
                     /locus_tag="ACHJWA_000213"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFO4306893.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(96994..97875)
                     /gene="rnm"
                     /locus_tag="ACHJWA_000214"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             complement(96994..97875)
                     /gene="rnm"
                     /locus_tag="ACHJWA_000214"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFO4306894.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAHIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HDGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            98013..98057
                     /gene="trpL"
                     /locus_tag="ACHJWA_000215"
     CDS             98013..98057
                     /gene="trpL"
                     /locus_tag="ACHJWA_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFO4306895.1"
                     /translation="MKAIFVLKGWWRTS"
     misc_feature    98034..98127
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            98149..99711
                     /gene="trpE"
                     /locus_tag="ACHJWA_000216"
     CDS             98149..99711
                     /gene="trpE"
                     /locus_tag="ACHJWA_000216"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFO4306896.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQSLSGNGEALLTLLDNALPAGVENEQLPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTETAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLESRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            99711..101306
                     /gene="trpD"
                     /locus_tag="ACHJWA_000217"
     CDS             99711..101306
                     /gene="trpD"
                     /locus_tag="ACHJWA_000217"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFO4306897.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPTNTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEHLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            101307..102668
                     /gene="trpCF"
                     /locus_tag="ACHJWA_000218"
     CDS             101307..102668
                     /gene="trpCF"
                     /locus_tag="ACHJWA_000218"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFO4306898.1"
                     /translation="MMQTVLAKIVADKAIWVEARKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVICDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLKMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFADGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVLNKAKVLSLVAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            102680..103873
                     /gene="trpB"
                     /locus_tag="ACHJWA_000219"
     CDS             102680..103873
                     /gene="trpB"
                     /locus_tag="ACHJWA_000219"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFO4306899.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            103873..104679
                     /gene="trpA"
                     /locus_tag="ACHJWA_000220"
     CDS             103873..104679
                     /gene="trpA"
                     /locus_tag="ACHJWA_000220"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFO4306900.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADTLELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPKKMLAALKAFVQPMKAATRS"
     gene            105060..105239
                     /gene="yciG"
                     /locus_tag="ACHJWA_000221"
     CDS             105060..105239
                     /gene="yciG"
                     /locus_tag="ACHJWA_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO4306901.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            105325..105825
                     /gene="yciF"
                     /locus_tag="ACHJWA_000222"
     CDS             105325..105825
                     /gene="yciF"
                     /locus_tag="ACHJWA_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO4306902.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            105871..106377
                     /gene="yciE"
                     /locus_tag="ACHJWA_000223"
     CDS             105871..106377
                     /gene="yciE"
                     /locus_tag="ACHJWA_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO4306903.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(106437..106562)
                     /locus_tag="ACHJWA_000224"
                     /pseudo
     CDS             complement(106437..106562)
                     /locus_tag="ACHJWA_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
     gene            complement(106786..107049)
                     /locus_tag="ACHJWA_000225"
                     /pseudo
     CDS             complement(106786..107049)
                     /locus_tag="ACHJWA_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165851.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
     gene            complement(107159..107376)
                     /locus_tag="ACHJWA_000226"
                     /pseudo
     CDS             complement(107159..107376)
                     /locus_tag="ACHJWA_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343765.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(107415..>107485)
                     /locus_tag="ACHJWA_000227"
     CDS             complement(107415..>107485)
                     /locus_tag="ACHJWA_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4306904.1"
                     /translation="IIDRRLQMRTNDFYSLLNRKYL"
BASE COUNT        26574 a        27975 c        26241 g        26695 t
ORIGIN      
        1 aattatttga ttaaggtttt acttaaggcg taacaaatga tatttatcgg ctaactgaac
       61 ttctccttta ggatgttttc actccccgaa atggggataa aaggcaaata aaataactaa
      121 aggatttatt caatgaaaag caaagtactg gcacttttaa ttcctgccct gctcggcgca
      181 ggtgctgcac atgcagccga agtttataat aaagacggca acaaattaga tctgtatggc
      241 aaagttgatg gcctgcatta tttttctgat aattcagcga aagatggcga ccagagctat
      301 gcgcgtctgg gttttaaagg cgaaacccaa attaacgatc aacttactgg ctacggtcaa
      361 tgggaataca atattcaggc aaacaacact gaatcttcaa aaaaccagtc atggacccgt
      421 ctggcatttg ccggactgaa attttcagat tacggttctt tcgattacgg acgtaattat
      481 gggctggacc gatatgctgc ctgaatttgg cggtgactct tataccaatg cagacaactt
      541 tatgactggt cgtgccaatg gcgtcgcgac ttatcgtaat actgatttct tcggtctggt
      601 aaatggtctg aacttcgcgg tgcagtatca aggtaacaac gaaggtgcca gtaatggtca
      661 ggaaggcacc aacaacggac gtgatgttcg ccatgaaaac ggtgacggct ggggtctttc
      721 cacaacatat gatttaggca tgggctttag cgctagtgcg gcatacacct cttctgaccg
      781 caccaatgac caggttaacc atactgcggc gggtggtgat aaagcagacg cgtggactgc
      841 tgggctaaaa tacgatgcta acaatattta cctggcaacc atgtattcag aaacgcgtaa
      901 tatgaccccg tttggcgaca gcgattatgc tgttgcaaac aaaacccaga actttgaagt
      961 cactgcacag taccagtttg attttggtct gcgtccggca gtctctttcc tgatgtctaa
     1021 aggccgtgac ctgcacgctg ccggtggtgc agacaacccg gcaggtgttg atgataaaga
     1081 tctggttaaa tacgccgatg ttggcgcgac ttactatttc aataaaaaca tgtccactta
     1141 tgttgactat aaaatcaacc tgttggatga agatgacagt ttctacgctg ccaacggcat
     1201 ctctaccgat gatattgtcg ctttaggtct ggtttatcag ttctaaatcc tcctgcccgc
     1261 tgttatggcg ggctttttct gcttattctt cctcctttga tctaaattaa aaatgtgaac
     1321 tccgtcatta cacaaaaagt gtcatctggc gttacacttt atgcggatac taaaacagga
     1381 ggttttatga acagaacgat tcttgtccct atcgatattt ccgattcaga attaactcaa
     1441 cgcgtgatta gccacgttga ggcagaggca aagattgatg atgcagaggt tcatttcctg
     1501 acggtaatac cttcactgcc ctactatgcc tctctgggtt tagcgtattc cgcagaatta
     1561 ccggcaatgg atgacctgaa agcggaagcc aaatcgcaac tggaagagat cattaaaaaa
     1621 tttaaactgc caaccgacag agtgcatgtc catgttgagg aaggctcgcc caaagaccgc
     1681 attctggaat tggcgaagaa gatccccgct catatgatca tcattgcttc ccatcgaccg
     1741 gatatcacca cttatctgct cggttccaac gccgcagctg tagtgcgtca cgcagagtgc
     1801 tccgtgctgg ttgtgcgctg acactaacgc ccgcacattg ctgcgggctt tttgattcat
     1861 ttcgcaaatg tgctgacatt ttcccctcta atccgtacca tacacgccac agtttttata
     1921 tcagatttct tatgctgggc gttccggcat gacgcatact cttctgatgc catataacga
     1981 attgagtcgc ttttaaatgt cgcaaaatca agaaattagc aagaaagaac aatacaacct
     2041 gaacaaatta caaaaacgcc tgcgtcgtaa cgtgggcgaa gccattgctg acttcaatat
     2101 gattgaagaa ggcgatcgca tcatggtttg cctctccggg ggtaaagaca gctataccat
     2161 gctggagatt ctgcgcaatt tgcagcaaag cgcgccaatc aatttttcgc tggtggctgt
     2221 taacctcgat caaaagcaac cgggcttccc ggaacacgtt ctgcccgagt atcttgaaaa
     2281 gctgggcgtt gagtacaaga ttgttgaaga gaacacttac ggtatcgtga aagagaagat
     2341 tccagagggc aaaaccactt gctcactgtg ttctcggcta cgtcgcggta tcctttatcg
     2401 caccgcaacg gaactggggg cgacgaagat cgcgttgggc caccatcgtg acgatatcct
     2461 gcaaacgttg ttcttaaata tgttctacgg cggcaagatg aaaggtatgc ctccgaaact
     2521 gatgagcgat gatggcaaac atatcgttat tcgtccgctg gcctactgcc gcgagaaaga
     2581 tattcagcga tttgccgatg caaaagcgtt cccgattatt ccgtgcaacc tgtgcggttc
     2641 acagcctaac ctgcaacgtc aggtgattgc tgacatgttg cgtgactggg ataaacgtta
     2701 tccagggcgt atcgagacga tgttcagcgc gatgcagaat gtggtgccgt cgcatctgtg
     2761 cgataccaac ctgttcgatt tcaaaggcat cacccacggt tctgaagtgg ttaacggggg
     2821 tgatctggcg tttgatcgcg aagagatccc actacaaccg gcgggctggc agccagaaga
     2881 agatgaaaat cagttggatg agttacggct gaatgtggtt gaagtgaaat aactcggata
     2941 gcgcccgatg cgcaaacgta tcgggctact ctaatggagg ctgaataaga cgcggtcagc
     3001 gtcgcatccg acaatcccga cgaagatgtt tacttttgca tcaggcaatt caacatttta
     3061 ttattttaat aaccgcaccc ggcacgtttt tcctttaatc ttcccgccct gtaactgttt
     3121 ccatgcttta tgagcaacag cctgacggac cgcgacatag acatgcgccg gatgcacggc
     3181 gattttgcca atatctgcgc catcaagccc gatatctcct gtcagtgcac ctaatacatc
     3241 acccgggcgc attttggctt ttttcccgcc atcgatacac aacgttgcca tttctgcttc
     3301 cagcgtcaca atggaactat tagctggtgg cgtttgccag ttaagtttta tctgcaacat
     3361 gtcagaaatg atattggccc gctgtgcttc ttccggagca cagaaactga tcgccagacc
     3421 gctatttcct gcacgagctg tacgaccgat gcgatgtaca tgaacttcag ggtcccacgc
     3481 cagctcaaag ttcaccacca gctcaagcga tttaatatcc agaccacgcg cagcaacatc
     3541 agtcgcgacc agtacacggg cgctaccgtt agcaaaacgt accagggtct gatcacgatc
     3601 gcgttgctcc agatcgccgt gtaatgacaa tgcactttgc cctacttcat tcagcgcgtc
     3661 gcagacagcc tggcaatctt ttttggtatt gcaaaacacc acgcaagagg atggctgatg
     3721 caagcttaat aaccgttgca acagaggaat tttgcctttg ctggatgtct cataaaattg
     3781 ttgttcaatg ggtggcaaag catctgttga gtcaatttca atcgccaaag gatcgcgttg
     3841 cactcgtccg ctgattgcgg cgatggcttc cggccaggtt gccgaaaaca gaagcgtctg
     3901 tcgagatgca ggcgcaaaac ggatgacatc atcaatggca tcgctaaatc ccatatccag
     3961 catgcggtcg gcctcatcca tcaccaaagt attcagcgca tccagtgata ccgtgccttt
     4021 ttgcaggtga tccagcaaac ggcccggcgt tgccacgata atatgcggcg catgttgcaa
     4081 cgaatcacgc tgcataccga acggttgacc accgcacaac gtcaaaattt tggtatttgg
     4141 cagaaaacgc gccagccgac gcaattcacc tgccacctga tccgccagtt cacgcgtagg
     4201 acacagcact aaagcctggg tttgaaatag cgacgcatca atttgctgta acaagccgag
     4261 gccaaaagcc gccgttttgc cgctgccagt tttcgcctgc acgcgaacat cttttccggc
     4321 aaggatcgcc ggaagcgcgg cggcctgcac cggcgtcatg gttaaataac ccaactcatt
     4381 aaggttcgtg agttgggcgg gaggcaaaac attcagggta gaaaaagcgg tcacaatcta
     4441 ttctcgtggt catcgacgca aagtcatcag gcgcgtatcc tcgcagatct acgctcacga
     4501 tgcgacaatt taatcggttc ttcatcgggt ggtgggtcag gcatgggttg cgggcgaggg
     4561 atcggatcgg gcactggaac aggatcgcca ggaatcggtt cagggacagg aatttgcaaa
     4621 taaataagtg tcgtcatatt tccctccggt cattgggtgg actcttaaag ggtagacgct
     4681 gataaataac aggcaaaaaa aagccgactc atcaaagtcg gcgtcgtacg aatcaattgt
     4741 gctatgcagt aattcaaaaa aggaagtaag acaatatgga gcgcaacgcc catcgcttga
     4801 cgttgcattc acctgcaaga gagatattgc cctgaatggg tagagagttt attgacttcg
     4861 ctcaaacttt gcggcgtttt tgtatacaga cagccggaaa aattgctttt gttacaacca
     4921 tttactacga tgcaaccata aagcaacacc accaataaga acaactaaca gaatacaaaa
     4981 aattgaaaat ccgaattgcc acccgccgcc agggatccca ccaaggttga cgccaaataa
     5041 ccctgtcaga aaggtactgg gtaaaaagac cattgccatc aatgacattg tataggtacg
     5101 acgagctaaa ttttcctgca tcacctgagc gatttcatcc gccatcacac cagtccgtgc
     5161 tatacaggcg tcgatttcgt caaggccgcg cccaaggcga tcggcaatat cctgcatccg
     5221 acggcgttgg tcatcgctca tccacggcaa acgttcactg gcaagacgag cataaacatc
     5281 acgttgcggt gccatatagc gacgcatcac aattaattgt ttgcgcagca gagccaggaa
     5341 tccacgcggc ggaatttgct gatcaaggag attatcttca aggtcgataa ttttatcgtg
     5401 cagctgctcg ataaattcgc tggaatgatc ggtcaacgca tcgcacacat ccaccagcca
     5461 tcccccgcaa tcggtcggac ccgtgccctc ttccagatca ctcaccacat cgtccagcgc
     5521 cagcactttg cgttgtcggg tcgaaacaat taaccgcccg tccatatata cacgcatggc
     5581 gaccagttga tcggggcgtt catcggtgct gccgtttata cagcgcaatg taatcagcgt
     5641 gccttcaccg agacggctga ctcggggccg tgtgctctcg cccgccagcg catcacgtac
     5701 gttattggga agcagcggtg ttgtcgccag ccattgggcg ctatcatggt gtacataatt
     5761 aaggtggagc cagcagggat gcgcttcatc aatcacatct gtattttcca gcggtttaac
     5821 gccgcctcta ccatccagca tccaggcaaa tactgcatcc gggacattaa cgtccgatcc
     5881 cttaatcgct tccacagtgc ctccatcatc aacgcattat tttgtagtct agccttctgg
     5941 ccctgttacg caacatctca tcaccccatt accctgaaat gattaataaa attctgtcta
     6001 aattgaatac aaaaagcaaa atgcttttcc gtatacaaac cgtgtgaagt gttaaatagc
     6061 gtctatcatt atcagaatta tctgatcata tgacgtggct tttttgcgat cggatagcaa
     6121 caaaaattga taaaaataat gggatctcaa tgattacgca caacttcaat actctggact
     6181 tactcaccag tcctgtctgg atcgtttcgc cctttgagga acagttaatt tatgccaaca
     6241 gcgcggcgcg actgttgatg caagacctca cgtttagtca gctacgaacc ggtccctatt
     6301 ccgtctcctc acaaaaagaa ctgccgaaat acctctccga tctgcaaaac caacacgata
     6361 ttatcgaaat cctcactgtt cagcgtaaag aagaggaaac agcattaagc tgtcggcttg
     6421 ttttgcgaga gctgacagaa acagaaccgg tgattatttt cgaaggtatc gaagcgccgg
     6481 caacgctggg tttaaaagcc agtcgctcgg caaattatca gcgcaaaaaa caaggttttt
     6541 atgcgcgctt ttttctgact aactctgcac caatgttgtt gattgacccg tcacgagatg
     6601 gacaaatcgt cgatgctaac ctcgccgcgc tcaatttcta tggttataac catgaaacga
     6661 tgtgccagaa acatacctgg gaaataaata tgctcgggcg tcgcgtcatg cctatcatgc
     6721 atgaaatctc gcatttaccc ggtggtcata aacctctgaa ttttattcat aagctggcgg
     6781 atggttcgac tcgtcatgtg cagacctatg ccggaccgat tgaaatttat ggcgacaagc
     6841 tcatgttatg tattgtgcat gatattactg agcaaaaacg gctggaggag cagctggaac
     6901 atgctgctca ccatgacgcg atgaccggat tactgaatcg gcgacagttt tatcacatta
     6961 cggaaccagg ccaaatgcag cacctcgcca tcgctcagga ttacagctta ttgctcatcg
     7021 acacagatcg ttttaaacac attaacgatc tctatgggca ttctaaaggt gatgaggtgt
     7081 tatgcgccct cgcccgcact ctcgaaagtt gcgctcgcaa aggcgatttg gtgtttcgtt
     7141 ggggaggcga agagtttgtc ttattactac caagaacccc actggatacc gcgctttcgc
     7201 tggctgaaac tatccgcgta agcgtggcaa aagtgagtat ttcgggctta ccacgcttta
     7261 ccgtcagcat tggtgtggcg catcacgaag gaaatgaaag catcgatgaa ctgtttaaac
     7321 gcgttgatga tgctttgtat cgggcgaaaa atgatggacg caaccgcgtg ctggcggcat
     7381 aagccacgga tgcgtctcga gatcaacgac tgcgcttagc gtggcgctcc cagttttctt
     7441 gctttgcctg tgccgtttta cgtagtgcga cgtaacacgc cccgctgccg ccatgatgcg
     7501 gtagcgcggt acaatatgcc tggacatcat caaattcggt caaccagcgc gccacatagc
     7561 tgcggacaat attggcatgc gatttatcat cccgcccttt accatgaata atcagcacgt
     7621 tacgcaaacc atccgccatg gcttgttgaa tgaaactgaa catcattttg cggcactctt
     7681 ccaccggctg gcgcaaaaga ttcaggctcg cctgttgcgg atatttacca ctgcgcagct
     7741 tatccagcac cccatgttgc aacccttccc gccgaaactc cagcggctga cttagcggga
     7801 tgatgtcgag aaatccggtg gtgaggaaat tatcaagttg cagcgtgtcg atacgctgcg
     7861 gcgcacgttg gttacgcgtt ggatgccagt ggacatcggt agcacgtttc agcggctgga
     7921 catcttccat ggcgtcaaga aacagcgatt tgtcgtcaag gttcatgtta catcctcccg
     7981 caattaagag cgcgatatga taaccagacc gggtcggtcc aacaacgtat tacccaaaat
     8041 tccagtaata agttccaaat attgccgata ttttaagcaa aatgcttatg catgattatt
     8101 cattcacgat attaataatg taacttatat tttcgtgaaa tctgtcactg aagaaaattg
     8161 gcaactaaag gttaaaaccg ttataacaca gtcaccggcg cagaggagac aatgccggat
     8221 ttaagacgcg gatgcactgc tgtgtgtact gtagagtctg gcggatgtcg acagactcta
     8281 tttttttatg cagttttaac tttgcagata gccgcattct cgccggaatt gcgtgattaa
     8341 tgacgcggtc agtggtgtct ggcgactatc acgccgctga atcaaataat aggccgcttt
     8401 cggtaaaatt tcgctaaccg gcaacatcac cagcccctgt ccgtgcaagg gatcgcagcc
     8461 catttcttca ggcagtatgc tgagaaaatc gctttttgcc accagactga tacaggctga
     8521 gaacgtctcg cagactacac cgacctgtgg cgtttgcgcc tgatcgtcaa gcaattcact
     8581 caactgtttg tagtagctgc cgtgtggcgt cggcattgtc cagctgtaat ccagtaactg
     8641 tttgatcgaa cgggcaccaa tggcggggtg tcccgggcgg caaaagatcg cgaattgctt
     8701 ttccagtaat ttctcaaaag taaattcgtg gtcgtacggt ccctgataat aggtattgat
     8761 ggtgaaatcc aattctccct gacgcaattc attaatcatc gacaccagtt gcccttccat
     8821 aatgcgtact tttacctgcg gatgctgctg atgaaaacga gatattacag ctggcatcag
     8881 actgcgggaa atactggccc ccatgccgat attaatctgc cctgccagtt gcccttgtcg
     8941 ttggcgaata tcctcttggg ctgcgcgcag ttcttcaaga ataagactgg cgtgctgata
     9001 aaaactttca ccggcatcag ttaacgtcac gcctttacta cggcgaaaaa agagttgcgc
     9061 cgctaaccct tcttccagct cctgaataga tttactcagt gccggttgcg acatattcaa
     9121 cattcggctc gctccgcgaa tgctgccctg acgagccact tcaacaaaag cccgaatttg
     9181 atgaattttt acctgaaaag ccatgacgcc accgataacc gttatttatc agaccaaaga
     9241 aactggcatc tactttaatg cagatgattg tgtcagggta atttatgaac ggttaaaact
     9301 gtgaaaaatc agttagtgat aagtaaaaac tatcgctacg tgaaccgggt cacacttttt
     9361 actgatgacg ggaaaggtta tggagtcttt gaatcaattt gttaattcgc ttgccccaaa
     9421 attatcgcac tggcgacgtg attttcatca ctatgcagag tctggctggg tggaattccg
     9481 cactgccacc cttgttgcgg aagaattgca gcagctcggc tactcactgg cgctgggccg
     9541 cgaagtagtt aatgaaaata gccggatggg actacctgat gaactcactc tacaacgcga
     9601 attcgagcgc gctcgtcaac agggtgcgct agcacaatgg attgcggctt ttgaaggtgg
     9661 tttcacgggt atcgtcgcca ccctggatac cggtcgcccc ggtccggtga tggctttccg
     9721 tgtcgatatg gacgcgctgg atctcagtga agagcaggat gtcagccatc gcccctaccg
     9781 cgacggtttt gcgtcatgta acgccggaat gatgcatgcc tgtggtcatg atgggcatac
     9841 caccattggg cttgggctgg cgcataccct taaacaattc gagtccggac tacatggcgt
     9901 catcaaactg atttttcagc ctgcagagga aggtacgcgt ggcgcgcggg cgatggtcga
     9961 tgcaggtgtc gtagatgatg ttgattattt tactgccgtg cacattggca ctggcgtacc
    10021 tgcgggcacc gtggtgtgcg gcagtgataa ttttatggca accaccaaat ttgacgcgca
    10081 cttcaccggt accgccgctc acgcaggcgc aaaaccagaa gacggttaca atgccttgtt
    10141 ggcggcagca caagccactc ttgcactgca tgcaatcgcc ccgcacagcg aaggagcttc
    10201 cagagtaaac gtgggcgtta tgcaggcagg aagcggtcgt aacgttgttc ctgcctcggc
    10261 gttgctgaaa gtggaaacac gcggggccag cgacgtcatt aatcaatatg tttttgaccg
    10321 tgctcagcaa gcgattcagg gcgcagcaac catgtatggt gtcggcgttg aaactcgtct
    10381 gatgggtgca gctaccgcca gttctccttc gccgcaatgg gtcgcatggt tgcaaagtca
    10441 ggcggctcag gtcgcggggg tcaatcaggc cattgaacgt gttgaagcgc ctgcgggttc
    10501 cgaagatgcc acattaatga tggcccgcgt gcagcaacat caagggcaag cctcctacgt
    10561 ggtgtttggc acacagctgg cggcaggtca tcacaacgaa aaattcgatt ttgacgagca
    10621 ggttctcgct attgccgtcg aaacgctggc gcgcaccgcg ctcaattttc cctggacgcg
    10681 aggtatctga tgcaggaaat ctatcgtttt atcgacgatg cgattgaagc cgatcgccaa
    10741 cgttataccg atattgccga tcaaatctgg gatcatccag aaacacgttt tgaagagttc
    10801 tggtcagcgg agcatctggc ttcggcgctg gaatctgcag gcttcaccgt tacccgcaac
    10861 gtaggcaata tcccaaatgc ctttattgct tcgtttggtc aaggcaaacc ggttatcgcc
    10921 ctgctgggag aatatgacgc cctggcaggt ttaagtcagc aagcaggttg cgcgcaacct
    10981 acatccgtga cgcccggtga aaatggtcac ggttgcggac acaatttgct gggaaccgcc
    11041 gcctttgccg ctgcaatagc catcaagaaa tggctggaac aatatgggca aggcggcaca
    11101 gtgcgctttt atggttgtcc tggcgaagaa ggcggctcgg gtaaaacgtt catggtccgc
    11161 gagggggtat ttgatgatgt ggatgcggca ctcacctggc acccggaagc ctttgccggt
    11221 atgttcaata cccgtacgct ggcaaacatt caggcatcat ggcgctttaa agggatcgca
    11281 gcacatgccg cgaattcccc tcatttggga cgcagcgccc ttgatgccgt aacgttgatg
    11341 accactggca ccaacttcct caacgaacat attattgaaa aagcgcgcgt acactatgcc
    11401 atcacaaata gtggcgggat ctcgcccaac gtggtccagg cgcaggcaga agtgctttat
    11461 cttatccgcg cccccgaaat gaccgacgtg cagcatattt atgatcgggt cgccaaaatc
    11521 gccgaaggtg cggcattgat gaccgaaacc acggttgaat gccgcttcga caaagcctgt
    11581 tccagttatc tcccgaatcg caccttagaa aatgccatgt accaggccct atcccatttt
    11641 ggtaccccgg aatggaactc cgaagaactg gcttttgcga aacaaattca ggctacgctt
    11701 acctccaacg atcggcaaaa cagtctgaat aatatcgccg caaccggtgg cgaaaacggc
    11761 aaggtttttg cactacgtca tcgtgaaacg gtactggcga atgaagtcgc tccatatgcc
    11821 gccaccgata acgtgcttgc ggcatcgact gatgtcggcg acgtcagttg gaaactgcct
    11881 gttgcccagt gtttcagccc ctgttttgcc gtcggtacac ccctacatac gtggcaactg
    11941 gttagccagg ggcgaacatc tattgctcat aaaggaatgc tgctggcggc gaaaactatg
    12001 gcagcaacca cactcaatct cttcattgat tcagggctat tgcaagaatg ccaacaagag
    12061 catcagcaag ttacggacac gcaaccgtat cactgcccta tcccgaaaaa cgtgacaccg
    12121 tcacctttaa aataacaaca acaacgcaaa cacaacaacc gaggaatgcc catgagtatg
    12181 tcatccatac cgtcgtcctc ccaatccggg aagctctatg gctgggtcga aagaattggt
    12241 aacaaggttc cccatccttt tttgctcttt atctatttga ttatcgtact catggtgacg
    12301 acggcaattt tgtcggcctt tggcgtcagt gcgaaaaacc cgaccgatgg tacgccggtg
    12361 gtggtgaaaa acctgctcag tgtggaagga ttacactggt ttttacccaa tgttattaaa
    12421 aactttagcg gttttgctcc acttggtgcg atcctggcgc tggttttagg tgccggtctg
    12481 gcggagcgcg tcggcttact gccagcacta atggttaaaa tggcatcgca tgttaatgcc
    12541 cgctacgcca gttatatggt gctgtttatt gcttttttca gccacatttc ttccgatgcg
    12601 gcgttagtga tcatgccacc gatgggtgcg ctgatttttc tggcggtggg caggcatcca
    12661 gttgcaggtt tactggctgc cattgcaggc gtaggttgcg gctttacggc taatttactg
    12721 attgtcacaa ccgacgtgtt gctgtcgggg atcagcacgg aagcggcagc tgcgttcaat
    12781 ccgcaaatgc acgtcagtgt aattgataac tggtatttta tggccagctc cgtagtcgta
    12841 ctgacgattg ttggcggcct gataaccgac aaaatcatcg agccacggtt aggtcaatgg
    12901 cagggaaaca gcgatgagaa actgcagaca ttgaccgaaa gtcagcgttt tggtttacgc
    12961 atagcaggtg tcgtatcgct actttttatt gctgcgattg cgctgatggt gatcccggaa
    13021 aacgggatat tgcgcgatcc gattaatcac accgtgatgc catcaccctt tattaaaggt
    13081 atcgtgccac tgatcattct ttttttcttt gttgtctcgc tggcttatgg catcgctacc
    13141 cgcacaattc gacgtcaggc ggatttaccg catttaatga ttgaaccgat gaaagagatg
    13201 gcgggattta tcgtgatggt ttttcccctc gcccagtttg tcgccatgtt taactggagc
    13261 aacatgggga aattcatcgc cgtggggctg accgatatcc tggaaagttc agggcttagc
    13321 ggcatcccgg cgtttgtcgg tctggcgttg ctttcctctt tcttatgcat gtttatcgcc
    13381 agcggttccg caatctggtc gattctggcc cccattttcg taccaatgtt tatgctactt
    13441 ggctttcacc cggcatttgc gcaaatcctc tttcgtattg ccgactcatc cgtattgcct
    13501 ttagcgccag tatctccttt tgttccactg tttcttggat tcctgcaacg ctacaaacca
    13561 gacgcgaaac tgggtactta ctattcgtta gtcttgccct atccgcttat ctttttggtg
    13621 gtatggctgc tgatgttgct ggcgtggtat cttgtgggcc tgccgatagg tccgggtatt
    13681 tacccacgtt tgtcttaaga gagaacggat gctgagatta cttgaagaaa aaattgccac
    13741 accactgggt ccactgtggg tgatttgcga tgagcaattt cgcctgcggg cggttgaatg
    13801 ggaagagtac agcgaacgca tggtgcagct gctggacatc cattatcgca aagaaggcta
    13861 tgagcgcatt tctgccacca atccaggcgg tttaagcgac aagcttcgtg aatattttgc
    13921 cggtaatctt agcattattg atacgcttcc cactgctacg ggggggacgc catttcagcg
    13981 cgaagtctgg aaaacactac ggactatccc ctgcgggcag gtaatgcatt acggcgaact
    14041 ggctgagcaa ttgggccgtc ctggcgcggc gcgtgccgtt ggtgcggcaa acggatcgaa
    14101 tcccatcagc atcgtcgtac cttgccatcg ggttattggc cgaaacggca ccatgaccgg
    14161 atatgcaggc ggagttcagc gaaaagagtg gttattgcgc cacgaaggtt atcttttgct
    14221 gtaaacatta aacaatttgt gccagcttgt tcacactttt atgtaaagtt acccttaaca
    14281 acttaagggt tttcaaatag atagacatat atttacatct aatatcggaa ttctctgctg
    14341 ttaaggtttg cttagactta cttgctccct aaaaagatgt taaaattgac aaatatcaat
    14401 tacggcttga gcagacctat gatcccggaa aagcgaatta tacggcgcat tcagtctggc
    14461 ggttgtgcta tccattgcca ggattgcagc atcagccagc tttgcatccc gttcacactc
    14521 aacgaacatg agcttgatca gcttgataat atcattgagc ggaagaagcc tattcagaaa
    14581 ggccagacgc tgtttaaggc tggtgatgaa cttaaatcgc tttatgccat ccgctccggt
    14641 acgattaaaa gttataccat cactgagcaa ggcgacgagc aaatcactgg tttccattta
    14701 gcaggcgacc tggtgggatt tgacgccatc ggcagcggcc atcacccgag cttcgcgcag
    14761 gcgctggaaa cctcgatggt atgtgaaatc ccgttcgaaa cgctggacga tttgtccggt
    14821 aaaatgccga atctgcgtca gcagatgatg cgtctgatga gcggtgaaat caaaggcgat
    14881 caggacatga tcctgctgtt gtcaaagaaa aatgccgagg aacgtctggc tgcattcatc
    14941 tacaacctgt cccgtcgttt tgcccaacgc ggcttctccc ctcgtgaatt ccgcctgacg
    15001 atgactcgtg gcgatatcgg taactatctg ggcctgacgg tagaaaccat cagccgtctg
    15061 ctgggtcgct tccagaaaag cggtatgctg gcagtcaaag gtaaatacat caccatcgaa
    15121 aataacgatg cgctggccca gcttgctggt catacgcgta acgttgcctg atttttccgc
    15181 ataactcact atccttctgt catatcatta aatttttctg atttattgat ctggcagaag
    15241 gttcatcact gtttcattca ccagatatgg gttaatcttt taattacaaa ctgcgttgac
    15301 agttgttgta aggagaccct gtatggctat gtatcagaac atgctcgttg ttatcgatcc
    15361 taaccaggac gaccaaccag cattgcggcg agctgtttat ttacatcaac ggattggtgg
    15421 caaaattaaa gcctttttgc cgatctatga cttctcatac gaaatgacca ccctgctctc
    15481 cccggacgaa cgtaccgcta tgcgtcaggg cgtcatcagc cagcgtacag cctggatcca
    15541 cgagcaggca aaatattatc tcaatgctgg cgttcccatt gaaattaaag tggtctggca
    15601 taaccgtcct ttcgaagcca tcattcagga agtgatcagc ggcgggcacg atttggtgct
    15661 aaaaatggcg caccaacatg accgtctgga agcggtgatt tttacgccaa cggactggca
    15721 cctgttacgc aaatgcccaa gcccggtgtg gatggtgaaa gaccagccgt ggccggaagg
    15781 aggtaaggcg ctggtggcgg tgaatctcgc cagtgaagag ccgtatcata atgcgctcaa
    15841 tgaaaaactg gtcaaagaga cgatcgaact ggcagaacaa gtcaaccata ccgaagttca
    15901 tctggttggc gcttatccgg taacgccaat taatatcgcg attgaactgc cggaatttga
    15961 tccgagcgtt tataacgatg ccattcgtgg gcaacatttg ctggcaatga aagccctgcg
    16021 gcagaaattc ggcattaatg aaaacatgac gcacgtagaa aaaggtctgc cagaagaggt
    16081 gattcctgat ttggcggagc acttacaggc ggggattgtg gttctgggca cggtaggacg
    16141 caccggtatt tcagcagcat tcctcggcaa cacggcggaa caggtgattg atcatcttcg
    16201 ctgcgatctg ttggtcatta aacctgacca gtatcagaca cccgttgaac tggatgacga
    16261 agaagacgat taacgactcc tcaataactg acaaggcccg agttatcggg cctatttttt
    16321 acttactgct cttcgccgcc aaggaaataa attcccaacg ggattgccag caacacagta
    16381 aagaccaggc tataaacaaa aatcatcgat gactgaatca catacattga tgtcgtccac
    16441 tccgacagcg gaatattgta ctgttctata acgccgccga tagtcgcgcg agtcacaacc
    16501 gacgccgcaa taatgaaaac cgccaacaaa ccaaagagaa atttcttccc tttcgctgac
    16561 ttcagtttcg ccataatcat gatgtaatcc ttgtgcagac aaatttcttg ttacctaaac
    16621 gtaacaatac catgacattt caccattgtc tgctctgaaa acctcacctc ttagctggat
    16681 ttacaatcaa cgcattgatt agttagctaa tgttgctact tccttccctg actattgatg
    16741 atctgaatga attgtcgcgg taaaatagcc ccgccagggg tttccggcat gacgacaatt
    16801 atctggagga gcctgataaa acaatgatcg ctgaactgtt tacaaataat gcgcttaatc
    16861 tggtcattat tttcggtagc tgcgcagcat tgattctgat gagcttttgg tttcgccgcg
    16921 gaaatcgtaa aagaaaaggc tttttattcc atgcggtgca atttttaatc tacaccataa
    16981 ttatcagtgc tgttggtagc attattaatt atgttataga aaactataaa ctcaaattta
    17041 tcactccagg cgttatcgat tttatctgta cgtccctgat tgcggttatt ctgacgatta
    17101 agctattttt gctgattaat cagtttgaaa aacagcagat taaaaagggt cgcgatatca
    17161 ccagtgcgcg gattatgtcg cgcattatca aaatcaccat tattgtggtg cttgttctgc
    17221 tttatggcga acatttcggc atgagccttt ctggcttgct gacctttggt ggtattggtg
    17281 gtctggctgt cggtatggct ggcaaagata ttctgagtaa tttcttttcc gggatcatgc
    17341 tctatttcga ccgtcctttc agtattggcg actggatccg ttcaccggac agaaatatcg
    17401 aaggtacagt agcggaaatt ggctggcgaa ttaccaaaat tacgaccttt gataatcgtc
    17461 cattgtacgt accgaactcg ctgttttcat cgatcagcgt agaaaaccca ggacgaatga
    17521 ccaaccgccg cattaccacg accattggtt tacgttatga ggatgcggca aaagtgggcg
    17581 ttatcgtcga agccgtacgt gagatgctga aaaatcaccc ggccatcgac cagcgacaaa
    17641 ccttactggt ttatttcaac cagtttgctg actcgtcatt gaatattatg gtttattgct
    17701 ttaccaaaac cacggtatgg gctgagtggc ttgctgcaca gcaagacgtt tatttgaaga
    17761 ttatcgatat tgtacagtca cacggcgcgg actttgcctt ccccagccag acgctgtata
    17821 tggataacat tacaccgccg gaacagggtc gctaaatcag acatcactgg cgttatccac
    17881 aagggtaacg ccagttccca tcacatcaat gcttcacaat atacatagtc cgactgtacg
    17941 ccacatcctc agggttatta atgggataac ctttcagcca cggtttgatt aatcgtccat
    18001 tggtatattg ataaattggt gcaatcggtg cttgctccat gaggattttt tctgccgcgt
    18061 tgtaatcggc attacgcgct ttaacggtat tttccgtcga tgcctgggcc agaactttgt
    18121 cgtatgccgg attgttaaag cgtgaaatat ttcctgaatg cgttgacgtt aataatgtca
    18181 ggaaagtgga gggttcatta taatccccca cccatgaggc gcggataaca tcaaaattgc
    18241 cggtgttacg gctatcgata taggttttcc attcctgatt ttgcaattta acatctacgc
    18301 caaggttctt tttccacatc gatgctacag caatcgcaat tttttgatgg ttttctgaag
    18361 tgttatacaa aagcgtcagc ttcagcggtt tttgcggacc ataaccagct gcgctcaaca
    18421 aagtttttgc ctgcgcattc agttcttcct gactcatttg ttcaaacggc gaaggttccg
    18481 gcgtaaatcc ggcggtaaca tctggtgtaa aatgccacgc tggcttttcg cccgtcccta
    18541 atactttttc ggtcatcagg cggcgatcta tcgtcatact taatgccaga cgaacgcgct
    18601 gatctgccgt cggccctttt tgcgtgttaa acgcataata ataggtcccg agctgcggcg
    18661 gcgtataaac ctgccccgga atatccttca acagcttctg atacatattt ttcgggaagg
    18721 attcggtgat atcaatatcc cccgcgaggt aacgcttagt ggctgcggat tcctgattaa
    18781 ttggcaggaa ggtcactttt tgcagtaccg ttttggcgtt atcccaataa tgggtattcg
    18841 gtaccacgac cagtttttca ttgactacgc gctctttaag aacataagcg ccattgccga
    18901 tcagatttcc gggtttcgtc cactctttac cgctttctac gttggctttt tgcaccggga
    18961 agaaggcaaa gttagcggtt aaattcacaa accacggcaa cggtttatca agctgaattt
    19021 tcaaagtatg gacatcaact gcggtgacgc caagctggtc aggtgtagct ttaccatcaa
    19081 taatcgcctg tgcgttgttg attcccgcca gcgcggcaaa ccatgcaaat ggcgatagcg
    19141 ttttcgggtc caccagacgc tgccagctgt agacaaaatc ttgcgccgtt accggtgtgc
    19201 catccgccca ttttgcgtta tcgcgcaggg taaaagtcca gatacggttg tcattacttt
    19261 tccactgagt cgcaacgccg gggacaatct cccctttctc gttctgattc accagtcctt
    19321 caaacagatc gcgaatgacc tgaatctctg gcaggcccac ggctttagcg ggatccagcg
    19381 acgcaggctc atctttaatg tggcgcacca gctcctgctt ctctgccagt actgtgccgc
    19441 tcggaacttc tgcagcatac gaaagagaaa tgctgctgac caacagcgca caacacgtga
    19501 ctgaaacaga gtgcttcata agtttccctc taaccgacat taaaattgat gcgtaattat
    19561 ttgtttcgcc ttaaagaaat gcaaacaact tccatcagaa agtgacacag gaagcataaa
    19621 atgttttatg tatcaatata ttaaatcaga tttagtgtaa ataatagacg ccaaaatgcg
    19681 gcaaagcaca tagaacaccg aacactacgt tcgggcttca cacgttcaaa ccagttttcg
    19741 gtatcataat cagcatctgt cgccattgta cgaagaataa aaaataacca aaacgttgaa
    19801 aggaactgag aatacgttaa gagaagaatt aaattaatgt atcagcaagt agtgctaaac
    19861 agcaccttta tacttcagcc tatctataaa taatgagaat gccgagccag catgtcgacg
    19921 gtgtggatag tacaggtgat aaccgggtaa atctggtgtg aatttatcca aaacacgtat
    19981 cagttttttt tctttaatcg cccgctcaac ctgatcataa ggtagatacg ccaatccatg
    20041 cccatcaatt gcagcatcaa tgatcaggtc tatcgtattc agtaaaagct gaccttccat
    20101 gcgaacacga acttcacgtc caccgcgtat taatctccag cgatttgctg tacccgatgt
    20161 gggaagatac aaattaattg cctgatgatc tattaattgt gacactgacg ttggaacact
    20221 tcggcgagaa aaataatccg gtgagccaac aatagccatt ggtatatctg gcccgattcg
    20281 aatggcgatc atatctttat ccatctcccc acccagacgg acgcctgcat caaaacgttc
    20341 agaaacgaca tcagtcaaac cataatcaat gataagctga atatcaattt cagggtgtga
    20401 tttcaggaat gtgcgcatgg ctggtaacaa tatcgttttt gctgcatgtt ctacagtagt
    20461 aatacgtatt gtcccggatg ggcggttctg cagatcgctc agggatgcca gggcagaatc
    20521 tatgtcatgc agcatagggc caagaacaga caaaagatgc tcgcccgctt cagtgggtac
    20581 aacactgcgc gtggttcgcg tcagaagccg caatcccaat cgttcttcta tacggcgcac
    20641 tatctggctt aaagctgact gcgccatgct caggcgggcc gctgcgcgag tgaagctacg
    20701 ctcctctgca acgacgacaa acgccatcag atcagcgatc tcttcacgct tcattcagta
    20761 tctccataca atctattaat catttaaatg ataggtctaa tcatgattta gggtctaatc
    20821 aacttaatga tcaatatcta tagtgatatc accaaccgtt aaaacgaacc aacaactcca
    20881 tgaggaacgg aatgatgaat aataaagtca gcttcactaa cagcaataat ccaaccatct
    20941 ctttgtctgc agtgatctat tttcctccaa aatttgatga aacccgtcaa tatccggcaa
    21001 ttgtggtatc tcacccaggt gggggcgtta aagaacaaac ggccgggacc tatgccgaaa
    21061 aactggcgga aaagggattt gtcactattg cttatgacgc atcttatcag ggtgaaagtg
    21121 gcggcgagcc gcgtcagtta gaaaacccat acattcgtac tgaagacgtc agtgccgtga
    21181 ttgattacct gacaacgctt tcttatgtcg acaatacccg aattggtgcg atgggaattt
    21241 gtgccggtgc aggatataca gccaatgctg caattcagga tcgccgcatc aaggccatcg
    21301 gtaccgttag tgcggtcaat attggttcga tgttccgtaa tggttgggaa aacaatgtga
    21361 aatctattga tgccctgcca tacgttgaag cgggttcaaa cgccagaacc agcgatatta
    21421 gcagcggaga atacgccatt atgccactgg caccaatgaa ggagtccgat gcgccgaacg
    21481 aagaactgcg tcaggcctgg gagtattacc acacccctcg cgcgcagtat ccaacagcac
    21541 cgggttacgc tactttgcgc agccttaacc agattattac ctatgatgct taccatatgg
    21601 cggaagtgta cctgactcag ccgacgcaga ttgtggcggg cagccaggca gggagcaaat
    21661 ggatgagtga cgacctgtac gatcgggcat caagccagga taaacgctat cacattgttg
    21721 aaggcgcaaa ccatatggat ctttatgacg gcaaagccta tgtggctgaa gcaatttcag
    21781 tattagctcc gttctttgag gaaactctgt aatgaaacat attcaaatcc gcaatagtga
    21841 tatggcctgg catattgctg ccaatattca gtttccacca aattttgatg agtcaaaaca
    21901 gtatccggcg attatcagcg tccatccttt tggcagttgt aaggagcaga cgtctggcaa
    21961 tatctatggc aaggcattgg ctgaaaaagg ctatgtcgtt ctcgcttatg acgccagttt
    22021 ccagggagaa tctggtgggg agcctcgttg gatagaagat ccgacacagc gcgtagagga
    22081 tattagccgt gttatcgatt atgcagtaac tctgccatat gttgatgctg aacgcattgg
    22141 cgtgctgggt gtgtgtgggg gggggtggct atgccattaa tgccgcgctg acagaaaaac
    22201 gaattaaagc tgtagtgagt attacgggtg ttaatatcgg gcgtcttttt cgggaaggtt
    22261 tcagcaatta cgatccgatt ggcgcattga atgcaatggc gtcgcaacga accaaagagg
    22321 ctcgtggtgg agaattacag atcaacgaat tacttcccgc cagcctggat gcagcaaaag
    22381 cccatggact gaccgaacgc gatgtttatg aagcaactga ctactataaa acaccacgcg
    22441 ggcaacaacc gggaggtgcg acaaaaatgt tgttctcaca tgcacagaaa acgctggcct
    22501 gggatgcctt cgcatttact gaagtgttac tgactcagcc ggtgatggta gtagttggtg
    22561 aaaaagtcgg tgccttcggt gcttatcgtg atggactgga agtctatggc cgggcgatgg
    22621 tttcgcagga tcgtcagtta gtttcattac ccgatttctc acattatgaa ctgtatgaca
    22681 aaccggaagc agttcaggag gcgctggaaa aagttattcc ttttttcaac acccatctgt
    22741 gataagtttc atgagaaata ttatgaaaaa tgtactcatt cttggtgctg gcggtcagat
    22801 agcccgccat gtgattaatc aactggcaga taaacaaacc attaaacaaa ctttatttgc
    22861 tcgtcagcca gcaaaaatac acaaacccta tccaactaat agccagatta ttatggggga
    22921 tgtactgaat catgcagcac tgaagcaggc catgcaggga caggatattg tgtatgccaa
    22981 ccttacgggg gaagatttag atattcaggc aaattcagtg atcgctgcga tgaaagcctg
    23041 tgatgttaaa cgtctgatct ttgttctttc cctcggcatt tatgatgaag ttccaggaaa
    23101 atttggcgaa tggaataatg cagttattgg tgagccccta aaaccattcc gcagggctgc
    23161 ggatgcaatt gaggcttctg gactggaata tacaattctt cgccctgcct ggctgacgga
    23221 tgaagatatt attgattatg agcttacctc acgcaatgaa ccattcaaag gtacgatcgt
    23281 atcacctaag agtgtcgcag cattaattac agatatcatc gataaacctg agaaacacat
    23341 tggtgagaat ataggcatca atcaacccgg gactgatggc gacaagccat tcttcatgta
    23401 aatcaattat ataaccacca tcgtactgta ctatgtgcag tacgatttat taaacttatt
    23461 aaggtgaact gatcaatcag atgcgattaa catgtgtaga accagtaaga ataaaatacg
    23521 ccatgtatat aacattacga caattctaat atctgcacat caatgaaaat acaaacaact
    23581 tccggcaaaa aagtgataca agatcgatga catagcgttg aagaaaagga tttgataatc
    23641 cgcaaaattt gcacaacact gcctgtaata cttcctgaac cagagacgat catgaccgta
    23701 acccgcccac gcgccgaacg cggcgcattt ccgcccggaa cagaacatta cggacgttca
    23761 ttattgggcg cgccgttaat ctggtttccg gcccctgcag ccagtcgcga aagtggtttg
    23821 attctggctg gcactcacgg tgatgaaaac tctttggtcg tcacgctctc ctgtgctctg
    23881 cggactttga caccttcttt gcgccgccat catgtggtgc tgtgtgtgaa tcctgacggc
    23941 tgccagctgg ggttacgggc caatgctaat ggtgtggatt taaacagaaa ctttccggcg
    24001 gcgaactgga aggaaggtga aacggtttat cgctggaaca gcgccgctga agaacgtgat
    24061 gtcgtattac tgacaggtga caaacctggc tccgaacctg aaacccaggc gctgtgccag
    24121 cttatacacc gcattcaacc tgcctgggtg gtctcattcc acgatccact ggcctgtatt
    24181 gaagatccca gacacagcga attaggcgaa tggctggctc aggcgtttga attgcctctg
    24241 gtaaccagcg tcggttatga aacgcctggc tctttcggca gttggtgtgc agatctgaac
    24301 ttgcattgca tcaccgcaga gtttccgccc atctcctccg acgaagccag cgaaaaatat
    24361 ctctttgcga tggcaaactt gctgcgctgg catcctaaag atgcaattcg cccgtcgtga
    24421 actgaagcgc cggttccaca tctaccgcca gccaggtcgg tccgtcaaga tcggcgaaac
    24481 tgacctgcgg caccagcggt aaagcggcgc taatggcccg agaggtacac aacatgcagc
    24541 ccagcatcag actgaaacct tgtgcacgcg cttcagtcgc cagcgccagc gcttccgtca
    24601 gacccccggt tttatcgagc ttaatattaa ccatctcata gcgccctttc agcgccttca
    24661 aattgctacg agtatgacaa ctttcatcag cacaaatcgg caacggatga ataaaattct
    24721 ccagcgccgc atcgtcctgc gcaggaagcg gttgttcaag catcgcaacg cctaaatccg
    24781 ccaatagctg gcaacgtgcc gccaaccctt ctgcatgcca ggattcattt gcatcaacga
    24841 tcagcgtggt atcgggcaca gctgtgcgaa ttgccaccat ccgctcactg ataagatggt
    24901 tatccagctt cactttcagt aatttcgcgc cggcctgcca gagtgttgat gcactattgg
    24961 ccatctgatc aggcgtaccg atgactaccg tctgtgcagt gataactgtc tcgggaagcg
    25021 ttatgccgat caaatcagcc agcgattgct gctgttttcg cgcggccaga tcccacaatg
    25081 cacaatccag cgcattacgt gctgcgccgg cagggagaat tttttgcaac tcctcccgtg
    25141 tcagcccttt ctctaattgc ggcacgacgc tcataatttg cgccattacc gaggcatcac
    25201 tttctccata acgcggatac ggcgtgcatt cgccggtgcc tttaataccc tcttcttcca
    25261 gttcaaccac caccacgcgc gcttcactgc gacttccccg ggcaatcaca aacggggtat
    25321 gtaagggcca ggcttcctca aataccttaa cggttctcat ctttactcct tggtgtcgcg
    25381 gtggtctgcg gattaagcgc aaatagcgtt tgctgtgtta ttgacagtta gcataaacta
    25441 ggtgtgacgt taactatatg taaacaggaa agataatcat gtcacaaacc gttcatttcc
    25501 agggcaaccc ggttacagtc gccaattcca tcccgcaggc gggtagcaaa gcgcagactt
    25561 ttactctcgt ggcaaaagat ctgtctgacg tcaccctcgg tcagtttgcg ggtaaacgca
    25621 aagtgctgaa cattttcccg agtattgata ccggtgtttg cgccgcatca gtacgtaagt
    25681 ttaaccaact ggcaaccgag atcgacaaca ccgttgtgct gtgtatctct gccgatctgc
    25741 cgttcgccca gtctcgtttc tgcggcgcag aaggtctgaa caacgttatc accctctcca
    25801 ctttccgtaa cgctgaattt ctgcaagcct acggtgtggc aattgctgat ggcccactga
    25861 aaggtctggc agcgcgtgcc gttgtggtta ttgatgaaaa tgacaatgtg attttcagcc
    25921 agctggtgga tgaaatcacc accgagccgg attacgaagc agctctggct gtactgaaag
    25981 cataagttaa tatgcctgat ggtgttgcac catcaggcat attcgcgctt actcttcgtt
    26041 cttcttctgg cttaagccat attcccgcaa cttattggca atcgcggtat gtgaaacgcc
    26101 gagacgtttt gccagtttgc gcgtgctggg ataattgcga taaagctggg ttaataccga
    26161 gcgttcaaaa cggctggtga tttcgtccag cgaaccttcc atcgcatctt cgcccacggc
    26221 taccgttgcg gcgtcataat ccggcaacaa aatatcctgt ggacgcagct cataaccgtc
    26281 cagttgtgtc agtgcgcgat agatagcgtt ctttaactgc cgcacatttc ccggccacgc
    26341 ataacgcgta agtacagtat tcaggtcagc ggccagtttc ggacgcggca cgccctgctc
    26401 gtcggcaaag cgggcgacga acagctcagt taacggcatg atgtcctgcg gacagtcacg
    26461 tagcggcggc aggttgagcg tcaacacgtt cagacgataa tagagatctt cacggaacat
    26521 gcctttttgc accagttcga ccagattctt ctgcgtagcg caaatcaccc gcacatcgac
    26581 atgcacctca tggtcttcgc caacccgacg gaaagtgcca tcattaagga aacgcagtaa
    26641 tttcgcctgc atccgtggtg acatctcacc tatttcatcc aacagcaccg aaccaccgtt
    26701 cgcctgctca aagaatcctt tcttcccttc cggagcatga ccaaacagct cactctcgac
    26761 cgcatcttcc ggtatagacg cacagttcag cgccaggtaa ggtttgctcg ctcttgggct
    26821 tgcctgatgg caggcgtagg caaaaagatc tttacctgta cctgtgtcac ccgtaatcag
    26881 cagcggcgcg cttagcatcg ccagtttctg cgcctgttcg acaacatgct tcattttcgg
    26941 gctgacagcg acaatttgac tgaaggcgct gacgtcctgg gcggcgacat tttgcaactg
    27001 acggcccata cgaatcgttg atcgcaacat caccaccgca cctgtcagga cgtgttgatc
    27061 attttcatcc tgaagataaa caggcgtaat ctccatcagg aagttctgtc cattaataac
    27121 aacatgctcg ttatgggaat cttgcggttc gctttccagc caacgtaaaa aattaaagcc
    27181 gttaatcaat tgtgcggcgg tatggttgcg cagtcgatcc aatttttgcc caaaaagctg
    27241 acagctcgcc gggttcgcca tatccacttt gcttttcata tcgacagaga gcacaggttc
    27301 aggcaacgcc tccagtaacg cgctcaacgc cagatgctca cgttcggaag gcatccacgg
    27361 gacagtacgc acatcggtaa cacctgcaat acggcgtatt tcggccatca gactgctgaa
    27421 actctcaaac tccagttcag caaaattgag gtagattcgc ccaatgggat caatctcaat
    27481 accgcgtaaa tcaatgcctc ttagcacgag tagatcgagt aattcgcggg tcagaccgag
    27541 tcggtcttca caaaagactt ccagacgcat gggaaccttc acctgaaaaa agaacaatta
    27601 atatgatgat atgacactat ttgaatagca ggaaggtttc tgtcaacaat cattgacaca
    27661 aagacggaaa aacgggcact tcggcgtaaa gatatccggt cagcacccga tattgcatta
    27721 tttttcgctg ggcgttttgc ctttttgcag cgtttctttc acctgaccga taagctgacg
    27781 acggaaatcc ccgaggcgag gtttgtcatc gtcaatccac ggcagcgggc ggcaaagctc
    27841 catagcttta atccccagtc gtgccgtcag aagtcctgca ccaatcccct gagctgcgcg
    27901 ggtagacaaa cgagcagcga gatcttgcga catccagtcc attcccactt cgcgcaccaa
    27961 ttcgctggct ccggcgaaag cgatattcag caataccagc ttgaacaggc gcaaacggct
    28021 gtaatatccc agttcaatgc catacagcgt ggcgatgcga ttaatcaaac gcagattgcg
    28081 ccaggcgata aatgccatat ccaccagcgc cagcgggctg accgcaatca tcaacgttga
    28141 ttcagctgct gagcggctga tttcgcgccg cgcctgggca tctaaaaccg gctggaccag
    28201 atgagcatac aggctgacca cttcacgatc gttctgcgtt tcatggattg aggcatacca
    28261 gcgttgcagc gctggatgag actgatcaat acccgcctgc tgcgccagtt tttcgcaaaa
    28321 tgcgcggcct ttgcccgtgc cgtggctgtg caacaaatcg cgcgcttcgt cgcgttcatg
    28381 ggcgcgctgt cgcaagcgcc ataagcgccg ccactctgtt accacagaac ctacgccagc
    28441 gccgataatc aatgccccag cagcacatcc acccagcgcc acccagtcct gagtttgcca
    28501 ggcattcatt gtccactgta caccctgccc gacaacgctt gcgccaaaca gagccagccc
    28561 gcccatcacc attttgcgcc acaggctgcg tttcggacgt aacgctgcgt ccattaccgc
    28621 ttcgacttgc ccctcttctt cctgcgcttc gtcgagcgtg gccggggcaa aattttgcgc
    28681 ctgattttcg tcaaaggtct gctgcgccct gaattttgga ttctgttcga cctccagcgg
    28741 accgtcgaaa tcaatacgtg gttttaacgg ttcggtcatc gcaatttatc tcctattaaa
    28801 aattccagcg cggcatcaag acgaatatgc ggcagcggtt tgtcgacatc catcacctgc
    28861 gggcgaaacg cttcaaactg gaacccttgc ttatcccaga acgcctgacc aggcaaacgt
    28921 gcgggaactt cgccaggata aacagtgagc ggtgcgccat cgctaagtcg attaccgcgc
    28981 agcgccggga ttttctcacc gttaacatca ataatgccac tggtggtcgc ctgaactgac
    29041 gccagcccca ggcaatccat gctgatccct tcgaacgccg cattttgcca ggcatcctga
    29101 attagttgtt gcagcaatga aaccatattg gcgtgctgat cgatggtcac atggtccgct
    29161 ttagtggcag caaacaatag cttatcgata accggcgaaa acaaacgcct gaacagggta
    29221 cgctgcccat agtggaaact ttgcatcagc tgcgtcagcg ccagacgcat atcattaaat
    29281 gcctgtggcc cactgttgag aggttgcagg caatccacca gcacaatctg gcggtcaaag
    29341 cgcagaaaat gattcttata gaaccccttc accaccttct cgcagtaata attaaaccgc
    29401 tcgcgcagca ttccggcatt ggtgtgctta tcggcctgcg ccagtttgga ctcgccccag
    29461 gcatcgacat ccggccacgg gaagaattgc agcgcgggcg cacctgccat atcccccggc
    29521 aagacaaagc gcccaggctg aataaagtgc agcccctgct gtttacagtg gtggagataa
    29581 tcggtccacg cggcggcaat gtctgccagc cggttttcgt cggcaggcgc tagcgggtcc
    29641 agcccttcgc acatcattcg ccatttcgcc gaccattcgc cgcgctgacc attgagtaag
    29701 cccgtcatct ggcgcgacca gcttaaatag tcctgcgcca gcatcggcag gtcgagcaac
    29761 cattcgccag ggtaatccac aatctccaga tacagcgtgg aggtatcctt aaagtggcgc
    29821 agcagcgaat cgttcgattt atagcgtagt gccaggcgaa tttcgctgac gccgcgcgtt
    29881 ggcgtcggcc aggcgggagg atcgccatac agctgcgcca acccttcgtc gtaggtaaaa
    29941 cgcggaatgc caaagtcacg ctgaggaatg cgttttacgc ccagcagacg ctcttcacgc
    30001 accgcactta atagcggcaa acgtgctccg gcatgaatat tgagcaactg attgaccatc
    30061 gcagtgataa acgccgtttt gccgctgcgg ctaagtccgg ttacggcgag gcgcagatgt
    30121 ctgtcgacac cccgattcac cagcgcatta agttcatttt taagtcgctt cattgctgtc
    30181 ctgtgctgct ctgaagacca gaaaaaaatc attcataagg atatgaatgt gggggccagg
    30241 ccattgatta tcaactaccc gtgttgtcat cgacgggcga actccataag atgtgcagca
    30301 ttcacgttcg gacggagttc ctgacctatg tcccctacta tttatgatat tgccagggtt
    30361 gcaggcgtat caaaatccac cgtatcacgc gtgctgaata agcaaaccaa tatctccccg
    30421 gaagcgcgcg aaaaagtgtt acgggccatt gaagaattac agtatcaacc aaacaagctg
    30481 gcccgcgcgc tgacctcttc gggttttgat gccattatgg tgatttctac ccgttcgacc
    30541 aaaactacgg cgggtaatcc gtttttctct gaagttttgc atgccatcac cgccaaagct
    30601 gaagaagaag gtttcgacgt gatattgcag acgtcgcaca ccccggcaga agacttacaa
    30661 aaatgtgaaa gcaaaattaa gcagaaaatg attaaaggca ttattatgct aagttcgcca
    30721 gcggatgagt cattttttgc ccaacttgat aaatacgata ttcctgtggt ggtgattggc
    30781 aaagttgaag gtcaatatgc ccatgtttat tctgtcgata ccgataattt tggtgacagc
    30841 attgcgttga ccgatgcgtt aattgaaagt ggacatcaaa atattgcctg tctgcatgca
    30901 ccgcttgatg tccatgtttc agtggatcgg gtaaatggtt ataagcagag tctggaagat
    30961 cataatattg cagtgcgtga tgaatggatt gttgatggcg gttataccca tgaaacagca
    31021 ctgcaagcag cacggcaatt attaagtcag tcaccgttgc ctgaggcagt gtttgccact
    31081 gacagcctga aattaatgag catttatcgt gcggcggcag agaaaaacat tgctattccg
    31141 cagcagttag cggtggtggg ttatagcaat gaaacgctgt catttatttt aacgcctgca
    31201 ccgggcggca tcgatgttcc gacgcaggag ttaggtcgac aaagctgtga gttattattc
    31261 cgcttaattg ccggaaaacc gtcaccacaa aatattaccg ttgccacgca tatgacgctg
    31321 aaataaatac cagcgacctt gcctgaatgc cggatgcgat gctggcgcat cttatccggc
    31381 ctacatttat agatcggata aagtactcgc gccgcatccg gcaatttagc ccattatcag
    31441 aacgagtaat ttacgccgac acctgcataa tggaatttat cgctgtcgcc ttcgtcgtga
    31501 tcctgccact caaacgcgta ttccagcgaa atggaaagac cattctggaa atcataaccg
    31561 taaaataaac caacacggtt aaaatcatgg ccttcacgtt caatatcatc ctgccagtcc
    31621 cagttactcc agcgatccag cccaatgcgc gtatacggcg tcaccgagtg gttgccgagc
    31681 gtcagcggca aataggcgcg aatttcttgc gtggaaaact caccgttatt acggctgtcg
    31741 tccatattga agccgcgctc gagataatag ttcactcgca aggcaaccgt ttcgttgaag
    31801 gtatattgca gacctgtttc cgtttcgaca cgggtatcag cgtaaccggt cgtgttcaaa
    31861 tcgttggcaa atttatacat cgacaaccaa ccgttgaaac gtaaatcgtc agtcagtttc
    31921 acatcccagt ctggcgcgat tttccagcgc tgcatattcg ccgtgtcttt acccggttca
    31981 tcaacgtagt gataaccata attacggaaa ccgccggtca ggccgaaact gaaatcatcg
    32041 ctttcgagga actgataatg tacctccagc tccgggcgat caaaccacgt tccacgttta
    32101 cccgcgctat aatctaccgg cccttcctga taataggcca gagaaattct ccacggcccg
    32161 ttggcggcat taaaatagac agaaggctcc gccagcccat ccatatcttc gccataaccc
    32221 tcgacgtttt ctatttcgta catcgcgccg atattaaagt gccagtcgtt cttttcctcg
    32281 gcctgtgcgc aggccattcc cgcacacatc accagtgcgg tacagggtaa taactttttc
    32341 attattgttc cttgtattat cctgggggaa tgccttcccc ctgtatttag cgaattgcta
    32401 tttccgtttc tgcatcaaag aaatgacatt tagtcatatc aaaatgaata gtgatatttt
    32461 ctcctgcatg ataatcattt aacgcaccag cacggaccac taactcgtgc cccccaaccg
    32521 tggtgtagag cataaattcc gcaccggtta attctaccac gctaattttg gcggaaatat
    32581 tattttcctc ttgcgcgtcc ggatgaatat cttccgggcg cattcccatc acgatgggct
    32641 tatgcaaact ttcctgtgtt ttcagaaccg ctaatttctc ttcgggaatg gttaatttaa
    32701 gcgtttccgt aacgaattta tcgccatcga tcgtgccgcg aataaaattc atcgctggtg
    32761 atccaataaa tccggcaaca aacatattcg cgggttggtt ataaacggtt ttcggcgcac
    32821 ccacttgctg aacaatcccg tctttcataa tcacaatccg cgtcgccatg gtcatcgctt
    32881 cggtctgatc gtgggtcacg tagatcatgg tggtgttcag tttctgatgc agcttgctga
    32941 tctctgcgcg catttgtacg cgcagcttgg catcaaggtt agagagcggt tcatccatta
    33001 aaaacacgcc cgcttcgcgc acaatcgccc gcccgagcgc cacacgctgg cgttgcccgc
    33061 cggaaagcgc gcccggcttg cgcttcaggt actcacgcag gccgagaatt tgcgccgccc
    33121 agttcacccg ctcatcaatc acctctttgg cgattttttg catcttcagg ccaaacgcca
    33181 tgttgtcgta aaccgtcata tgcggataca acgcgtagtt ctggaacacc attgcgatat
    33241 tgcgtgcttt ggctggaacg tcattcattc gtttgccgtc gatcaacaga tcgccgccgc
    33301 tgatctcctc aagcccggca atcatgcgca gggtggtcga tttaccgcag cccgacgggc
    33361 cgacaaatac gatgaactct ttatcggcaa tttccaggtt gaagtccttc accacatgca
    33421 cctggttatc gtagattttt tgaatatgtt gtaacgaaag ctgagccatg ttgattcctt
    33481 tgctatacgt tttgccagaa ggccgataac cgcggccagg tcagggagtc cgttgagggc
    33541 aacaataatt gcgccccggt taagcctgcg ccgatcccca ccgagcgcat accgctggcg
    33601 ttaatggcgt caatgcccgc ctgcgcatct tcaatgccga tacatgcctg cggcggcacg
    33661 cccagcccgg cacaggcagc gagaaagatt tccggatccg gtttcgagtt tttaagttgg
    33721 gaagcatccg cgcagaaggt gaaaaactcg cgcagctcca gcgccgctaa aatcgtcggc
    33781 gcattcaggg agacagaagc cagccctacc gggatctgct gtgcgcggag ctctgccagc
    33841 aaattgcgaa taccgggcag cacagcatta accgtcagct cgcgcagtga gtggacgtaa
    33901 agcagatttt tgcgatacgc cagttgcgtc cgctcctgcg ggttaaagtc gccctctttg
    33961 cccccgtgtt gcagaatgcg ccgcagagac tcatcgcggc tgatcccttt tagggattcg
    34021 ttaaactgcg catcaatgct gatgccaatt tcagcggcaa tctgctgcca cgcctggaaa
    34081 tgcagatgcg cggtatcggt gattacacca tccagatcga aaattacccc ttgcagtttc
    34141 attcatcctc ctgatgcgtg gtagcggtcc cattgaaggg caaaataaaa tcacccaaac
    34201 agaaaacaga ttcttcagcc acggatataa gctgcccgtt caaacgcagt gaaacgggcg
    34261 cagaagttcg aatcgcaata cgctgcgcgt cgagcgtgac ctgtaattcg cagccctgcc
    34321 agaacagagg gaaagacaac tgttgccact gctccggtaa cgccggattg agatgtaatt
    34381 caccgtcacg cacgctcacc ccggcaaaac cctgaatcgc ccccagccag atagcgccag
    34441 ttgcggcagc gtggatacca tcatcacaac tatgcggatc agcaccaaga tcgatttcag
    34501 tcccctcgcg ccagaactga taactttggg tcagcaggcc acagcgtgcg gcaacaatgc
    34561 cgtggattgc tttacttaat gacgagtcgt gaatagtgcg cggttcataa aattgcagat
    34621 tggcaagaca cgataccgct gagaactgct ccggcagcat gtaattgagc atcaccacat
    34681 cagcttgttt gaggatctgc atctcgttca cttctgcgcg tgaataatcc agcagaatgg
    34741 tttgcttccc cgccgccgct ttgtatttag ccagattaat cgccggctta gccataaacg
    34801 aatcatcctg cggcaaaacg ccatcgggct gcgtttctgg catccatagc tctttgagga
    34861 acatttcggc gcgatggata aacgcatcgt cgctacagcc gaactggcgg gcaatattca
    34921 gcgcctgttg aacgttgtag cgggccatat agctggtgaa ggcgttatta ttgacatgtt
    34981 cggtatattc gtctggccca ataacatcat gaatttccag acggtcgtta accctcaccg
    35041 cgcggctaat ccagaacttt gcagtttcca gaagtagcgc catgccttca tgagcaatga
    35101 aactttcatc ccccgtggtc tgccagtatt gaataaccgc ccaggcgata tcggccacca
    35161 gatgatgttc cgcctgcgcc gaggccactt tttgccgcag cccggtacga atgttaatgg
    35221 cggcaaattc tggcgtctct tcttcgccgc tgcgcgcgct ttcccacgga aatagcgcgc
    35281 cctgccagcc gctgcgccgt gctttctcct gcgcgcctgg caagttgtgc cagcgataac
    35341 gcagtaaact tcgggcaacc gtcggatcgc taaacagatg gaacggcagc aaaaatactt
    35401 ctgtatccca gaaaacgtgg cctttgtagc cttcgccggt taagcctttt gccgcaatac
    35461 tgctgcgctc gtcgtgagcc ggcgtcatga tgcgcagatg ataaagcgca taatctaacg
    35521 cttgctgatc gtgcgcatcg ccgccattta ccgtgatacg acgtttctgc caccattgac
    35581 gccagttttc tgttgatgct gcaagaagtt ggtcgtaact ctgctgcgcg catatttcaa
    35641 gctggcgaag cgacgcgctg ccccactcgt ctaaaacggc ttgcctgtca tcccgccagt
    35701 cgatccacac cagtttttgc aacgtcactg tctcgcctgc atgaagctgc gcactggtat
    35761 gttgcagcaa acggcgctct ttggcggtat agcattgctg cacatcaccg ctcaccttac
    35821 agcaacagct gatggccaca tcactgcgtc catcctgggt ggtgtagatc ccctgcatca
    35881 gatgctgacc aaacacccgc acctgggttt cgtcgagatg ttggcgaccg tggttggttt
    35941 gcgtggcgtc gatacctgtt gaaatcagca ctgacgcgtc ggcgtccagt ggcgtaatag
    36001 tgatttccag cgcaatgagc ggcagttggt ctgccgaaac aaagcgacgg ctggcgatgg
    36061 tgtaacctgc gccgttgctg gtacgccaga caacgttgcg gcgtaattcg ccactggcaa
    36121 agtcaagctc acgctgccag gcttcgtggg ataacgagaa aacctcacca tttatggcaa
    36181 tctccatccc caagatatca ggcaggttca ccagttcgtt gatttcacct tttcccgccc
    36241 gatgatacag ccccgccaga tacatccctc gcgtctggcg cgtgtaatct tcttcatggc
    36301 tggcgcgaag cccaagatag ccgttcccct gcgccatcag cgatgcatac ttgttcaggg
    36361 tatgctggct gaaatggggt tctgataacg ttactggcct ggtcataatt ccacacgcgt
    36421 ccctgtttct gcggattgat ataacgccgc caccagttgc tggatgatgt acccctgctc
    36481 ggcgtcggct atcatcacgg gcttgccctg tacgtgattg ataaaggctt ccatgctgcc
    36541 caaatggcgg ttgtcgtctg ccatttcccg ttgcatcagc gtcattaatt caccgttgtt
    36601 atcggtgtag atatgtgctg gaaacagcgt cgcaccggct ttatcaccac aaaagctgac
    36661 gttcataatc gactgttcgc ggatgttgag cgcaaatgac gtttccagcc acagaatgcc
    36721 gccgttatga aattcaatgg tgccaaacag cgaatcttcg acgctgtaag ttgccggatc
    36781 ccactcgcca aattgaccac agctcttttg cgtgccgatc ttttgaaagc tatgcgcatt
    36841 cacgcttttc accgcaggat agcccagcac atacatcgca gcatccagca tatgaatgcc
    36901 gatgtcgatc agcgggccac caccctgcag ttctttattg gtaaagacac cccagccggg
    36961 aacgccgcag cgacgcaggg cgcgggcggt ggtaacgtaa atctctccca aaacgccgtt
    37021 ggtcacctgt tcacgcagct gttgcgtatc gagcgcaaaa cgatggtgaa agtcgtaggc
    37081 cagcaccttg ccctgtttgc gcgcggtatc gcacatttcc cgcgcctgtt ctggcgtcat
    37141 ggcgggcggt ttttcgcaca tcacatggca gcccgcttcc agcgccatca gggtatgttc
    37201 gtaatgaaaa cggttaggtg agcagacgct aaccacatca ggtttcaccg ccagcagcat
    37261 ggcctgcgga tcgtcccaca cggatgcatt cccgtatttt tctgccagcg cctgcgcctg
    37321 ggaaaggcgg ctgtcacaga cagccaccag ttccagatcg ttgcgggtgc agtagtacga
    37381 agcatgaacc ttatccgcca cctggcctgc gcctattatc gcgacccgca gtggagagct
    37441 tgtcattgca cttttcacaa agacctctta gcaggtacgc aaccaggtca acgaatcacg
    37501 gtacgcctgg gcaggatctt ctgcccggat acgcccttca tacaccacat aaccctgata
    37561 gttatcggcg cgcagctgtt caaacagcgc gtggaaatcg agggtgccgc tgcccggctg
    37621 gtaacgatgg ttatcggcaa tatgcacatg accgagcaag tcgcggttgt catgcagcgc
    37681 ctgcgccagg ttatcttctt cgatgttcat gtgatagaaa tcgccgataa tctgcacatg
    37741 tttaagattg ttttcgacgc tgtaacgacg agcatcggcg agggtgttga tcatatgatc
    37801 ctgatagcgg tttaacggtt cgagatacac cacggttccg gtacgcgcgg cgacctgttc
    37861 cagtacgcgc agggaatcac tcaccatttt gcggtcgccg tccaggctac gcggcgaggt
    37921 catcggcggt aagcggaagg taaacatgcc ccacgctgcc ggaacgacga tacctttacc
    37981 ccccacttct gccagcgctt cgagaatgcg ttcgatctgc tttaagccat taagacgacg
    38041 ctcttcgata aagtcaccaa tccagccgtc atagccgccg caggcggtgg tcaccggtaa
    38101 accggtttct ttgatcgccg atttgacttc ttcgatgttg ttgaccagca gtttgccgtc
    38161 aatctcaaaa ccatcgaagc ccatctcttt gatataacga aatttctcca gaatattttc
    38221 cgggaagaac gcctgattct gtgtgcctat tttcattttt tcattccttt gaattaaaac
    38281 gtgacgccca ttttgatgct ctgttccgga tgctgatcga catactgcat gtagctttcc
    38341 gggctgttgg caaaggtcac taccgggtcg attaaatctt cgcaattgag ataaccgttc
    38401 atcagcagtt tccagcaggt ttcttcaata cgcttacggc tccagcgcgg ataatccggg
    38461 ttcggttcac tgcacgcgcg agagaagaca attttggcgt tattgaaatg cgcttcgcgt
    38521 ccgaggttaa aaccttcagc aaacggttta gcaaacgcga cataggagat ggtgccaccg
    38581 taagccaggc cgcgcagcgc cgactgcagc gcgtcggcgt aaccgctggt ttcgataatc
    38641 acatcggcac cctgcttgcc ggtcagcgtt ttgatctctt tgcctacgtc agtgccaatg
    38701 ggattaaggc agaaatccgc accgtgacga cgagcaatat cacaacgatg ggcaatagga
    38761 tcgacgccaa ttaccaccga cgcgccagcg cgtttagcca gttggatggc aatttgaccg
    38821 atcgcgccaa gccctaccac cactacaaaa tcccctacgc gtacgttggc atcgcgaacg
    38881 ccactcatgg caaactgcgc cgggtcgtag cagacagcgt ttttccagga gctgccttcc
    38941 ggcattttgc gcagcttgta gttattcact gcgttaatga tgatcgtctc ggagagcggg
    39001 ccgtagccgc atacgctgtc gcccaccgcg tagtcggtaa cgtcgctgcc gcactcgata
    39061 atgtcgccaa ccaccatgtt gccaagctgg aatttgccaa actcaatgcc gcgcggcgca
    39121 tctgccggac gcggggtgaa catctgccat tcgccgttaa agtcttcatc gataaacggg
    39181 ctggcggcgc ggaagtcgac cacttccgtt ccgtgtttcg gtgcaccgaa acgcacgcgg
    39241 atcttcactt cattggctaa aatcgcccga tcttcatact caaccagcgc agcaacgcgc
    39301 ggtgctgtgg ctactaactt tttcatgtaa aactccttgt aaagaaactg gccgctttag
    39361 cccttcacgc caccggcggt caaaccactc ttaataaaac gttccgaaag cgcatacata
    39421 atcaccaccg gcaatgcggt caccagcgag gccgccatca tccgtcccca gatgtaatct
    39481 ggcgtactga acagcgcgtt caggccgact ggtaaggtga aattgctggc gctggagagg
    39541 aaaatcgacg caaacagata gtcgttccat gccaccataa agcagtagac gaatacggat
    39601 atcagcccgg acatcgccag gggcacggtg atacggaaga taatttgcag acggttgagg
    39661 ccatccatca tcgccgcttc ttcgatctca tcagggatgg tgtcgaagta acttttcagc
    39721 ataaacacgg cggtgggcag agtttgcgtc accatggtaa tgatcagcgc catctcggtg
    39781 tcgtaaatgc caagtgcggt gataattttg aacagcggca ccaccagcaa aatgccggag
    39841 aacatataca ccgtgtaaaa gctggcgttg atggtcatcc ggcctttaaa gcgcaggcga
    39901 gaaagcgcgt aagctccgag aatgccgaga aataccgcca ccacggatga aaccaccgac
    39961 accaccatgc tgttacggaa gtagtcgaca aacgggaaaa tcaccgggtt aaaaatgtcg
    40021 acataatgct ccagcgtcca ctgttgcggc agcagcgtag gatgcagcga gatcgcctct
    40081 tttgcgctct tgaacgaggt catcagcatc acaaaaaacg gaaacagcgt gatgatgaga
    40141 aacagcgcca gcccgcggta aaaaccgata cgactgagtg tgcgtttatt tgttgccatt
    40201 gaggttcacc cgtttcctgg tcagcagaat gacagcgaaa ataatgatga agagcaccac
    40261 cgagatcgcc gccgctttcc ctaagtcatt aaaggcaaag gcggttttgt agagatagac
    40321 accaagaata tcgaccttgg ttgtcagcag ataaacatcc gcgaacatgt agaacatcca
    40381 gatggtgcga agggtcacca ccgtcgccag gaccggcata atcgcgggca gcgtgacgat
    40441 acgaaaccgt tgccaggcat tagcgccatc catctctgcc gcttcgtaca gcgatttgtc
    40501 gatggtctgc aaaatcgcca gaaacgagat aaaggcatac gggaagtagc gccagatggc
    40561 gaagatcacc accagcacaa aactactgcc cggattatcg aaccacagcg gtgcctgctc
    40621 ataaagatgc agaagatcga cgccgaggta gttcacaatg ccgtagccgt tgttgaacat
    40681 gtatttccag gcgaacacca gcgaaataga cggcgttacg taggagagga tcaccagcga
    40741 acgcgcggtt ttgcgcaggc ggaactcgcg gttgaaaaac atcgccactg ccagcccgag
    40801 cacggtgctg cccgccacca ccaacgcggt ataccagacc gtcatccaca gcgagtgcca
    40861 gaagccggga tcggagagaa tacgcacata gttgcttacc ccaacaaacg ttgactcgat
    40921 gttgggattg agcggcagac gtaaaaaact aatttcgata ttcgacacca ttggccacgc
    40981 aaccagaccg cccagcagta ataagctggg tgcgagaagc aacagcgcaa agggcatatc
    41041 ggaacgacct gaaaaaagcc tgttcattgc ggattcctgt cctgggttta gtgctgttcg
    41101 atcagttcat ccagcttttt ctggctcgtt tgcagcgtgc cagggagatc ggctttacct
    41161 acggtgacgt tatgcaccat ggagctcacc accccagacc ccgtcacatc gcccatgcgg
    41221 gtaaattttt tatcccctac tgcgccaaag acctgaatat tcggcagttc accgattaac
    41281 tgatttggca gttcacccag cgccttaata acgtcgttgt ctttccaggt ggaagtagtg
    41341 acaaccgctt tgttcaccgg cagcgccgcg cctggcgaca tcatcaccca gtcggcaatg
    41401 ttgtctgcct gctccataaa ggtgacaaat ttttcagccg cttccgtttc ttcagtcttt
    41461 tgtccggcgg taatggtcag cgaggtcaac atgccgtaga cggcagagtt tttctcggtt
    41521 ggcaccacga aaccgacgtt tttcggatcg ccttctttaa tcacagccgg aaggatatag
    41581 gtggagtaaa tcgccatcgg cgcggtgccg ttcataaagg cgtctttcac ttccatgatg
    41641 tcgttagaac ccggcatggt gttggcagca aggtcgcggt aataggtcag cgcctgcatc
    41701 atctctggtg aatcaagggt gattttgcct tcggcgttaa agacgttggc ctagttggat
    41761 aacgcaaact gggagaagga ttgttccgtc agcacgcttt ctgccgtagg cagcgcaata
    41821 ccgtattttt tattcgccgg gtcattcagt ttctgtgcaa cgtccagcag ctgttgccag
    41881 tttttcggct cctccagccc cgcttttgcc agcacatctt tgcgatacca gatgccgcca
    41941 atccaggcgc tgacaggaac accggtccat gcgctaccat cttcggtacg cacaatacgc
    42001 agtacgccat cgtaaaacgc gccttcacca acgttgctga tgaccgtggc aaccgccttg
    42061 cgatcgataa gctgctcttt gtccataact ttggcgtagt catgactggt ttcaatcact
    42121 tccggcagcg agccgctacg tgaaagagtg atgactttgg tgttataagc gtcctcttcg
    42181 acgggcactt gtttaacggt gatacctggg ttttcttttt caaaacgggc aatcaatttg
    42241 ctgataactg cctgacgctc ctgctccacc gaagaatgca taaattcgat ggatacatgg
    42301 ggtttatttt cttctttaca gcctgaaatc agggcgcagg aaaccagtgc tgataacagc
    42361 acgatttttg atttaatcat gagatgttcc tttttattta atccacataa cctgccaggg
    42421 gcgcagcgtt attttattac ccaatatatc aacttcacta atcagatccc gaccgtgcag
    42481 gccagtaata tttacatgct gaatattttt actgacatta aacaatccgg tcagacaatt
    42541 accctctgcg ttacttcttt gaatacgcat tacggatgaa ttaaccgtat cgatggtaaa
    42601 attattatcc ggatgaaact cgttatggct gcgacgaagt gtaattaaac gcgacaactc
    42661 atgatatacc gcatgcctta atgtagcttc atcgttcagt tctcgggtta tctcttcact
    42721 gtgatatttt ttacggttaa tcgcacggtt atatccgagt ttttcgacac ctgcgtaatc
    42781 attacgcgag cccagaatac tttgaatata tatcgctggc acaccgggga aacttaacaa
    42841 aatcgcatgg gcaaggataa acctggcgca acgttcttca tcgctactct cgcggcggct
    42901 taacgcatcc atataggtga catttatttc atagggactg cgcgtaccgt cgggattatt
    42961 tttccagttt actaatgcac cttcctgctg taatgcctcg accagcgcta atatttcgct
    43021 ttctggtaac aagccccgca gtgggttcag cccgatgcca tcgtgagagg cgaggaagtt
    43081 aaaccaggtc gttttaccgg agggcaagct cagactttgc gcccactggc acagcgcctc
    43141 gacgttctgt ttttgcaccg catgtaacac cagcggcggc agcgagaact ggtacaccat
    43201 atgcgcttcg tcatcgcctt cgccaaagta agcaatgtta tctttatgcg gaacattcgt
    43261 ctcagtaata atcaccgtac ctggcgcgat gtcatcaata atcgaccgta acagtttgat
    43321 aatcagatgt gttttttcca gatggatgca gcttgttccc ggctctttcc acataaagcc
    43381 aacggcatcc agacggacat attctgcgcc cttttccagg taacaaagca aaacatccac
    43441 catcgccagc aacacgtcag ggctacggta attcaggtca atttgatcgt cgctaaaggt
    43501 ggtccataaa tggcgcgtgg aattatcatc catttgaaac ggcgttaata gcggtaatgc
    43561 acgcggacgg gtgacagcgc tgagatcggt ttgcggatca accgcaataa aaaaatcttc
    43621 aaaacctggc tgctgttgta aatagttttt aaaccattca ctttttgccg acatatggtt
    43681 gcagacaaaa tcaaacatta aatgactgca ttcaccgagc tgctgaatat cctgccacta
    43741 cccgcttcac tggcgacctg atgataatca attaccgaaa atccatcatc agatgaccat
    43801 ggataaaaag gtaataaatg aacatgtgaa aaaatgcttt gcagccattg atggtaaaac
    43861 tgattaaatg tgggtagtgg ttttaaatca ttactgtgaa attgatcggc ataggtaatt
    43921 aacacgacat cactttcatc ccagtgtttt ttacgtcgct gtgtaattaa tcgtttcgca
    43981 ctctcaagac gcgttacaag tttctgtaaa tgagttgcgg taaatgttcc accgtagatt
    44041 tcgtccaggt aatccgtaat tttctgtttc atgttgcctt tcctggtagc ggtcccatga
    44101 aatgagagac tactccgcag aaaataaact gtcaattgag cacaagggtt ttagtcacgc
    44161 aggaaatcat tttttaccag ggaaaaagcg tatttgtgag ctgacgcaaa taatctatag
    44221 ggatgtaaat aaggtgtgac agaaaaaaac ggcgcataag cgccgctcat ggtgaattct
    44281 tttaaccttt gaccttcggc attgcgatgt ctttcaggcc accggcgttc tcaacgtggg
    44341 tatatcccat ctcgctaagg atctcttttg cctgccctga ttggcgtccg gcattgcaat
    44401 acactttcac ggtgtcgttt ttatccggaa cgacggtggc aatgcgctct ttcacttcct
    44461 tcaggggaat attgatggcc ccctgaacgt gctcttgctg atactgctct ggaacacgaa
    44521 catcgatcca gtgttcagcg gcgaaaacgg gcagtgaaaa caccagtgcc agagcaagta
    44581 agcctttttt aaacatgtaa gcgtccttct atggatgaat attgctatcc attatacgcc
    44641 tgagatttaa caatttttgc gaaactcccc tcacctttta taacgctgcg ccagtttatt
    44701 ggcagcccga cttaacagcg gttccagcgc cacagccagc aacattttca gcggtcggcg
    44761 agcaactgat tttatcgccc agcccgccac acccgctggg ccatagcgca gtgcggtaag
    44821 aagtaccagc ttgcctgcta atttgaaacc aggctttacc ttttgctcgg cctgttgcca
    44881 gcgagtattc ataactttcc tcacagttga cggaaacggc tacgtaacgt gaatgtatcg
    44941 gaagtgacat aacgttccat ctcgcgtaaa cgcgtttcac ttgccgccag ttcacggtcg
    45001 acttcatcca gcaattcact gctggaaggt agctgctcac caaaggccat gttgtccggc
    45061 attggatcaa gcgcaaatga caaaatgatg taagcaacca gggtaaacag cgccagaccg
    45121 aagaaaatcg acaacaccac caggatacgc accagtttta ccggtacatc aaaatagttg
    45181 gcaatcccgg cgcagacgcc acggaccatg ccctgctgtg gaatacgcca taatttttta
    45241 ttgagattaa tgcccgccat tagcgatccc tccagttcgg atgttctgca tcaagaatag
    45301 attccagcgc ctgaatacgt tcgcgcatcc gttttgcttc atcagccagt tgcgctaatc
    45361 gctgctgctc actttgcgac aattcactgc gaccagaacg attgctgtaa tgcagccata
    45421 accagatcgg taaaacaaac agcacaaaaa tggttaatgg aatagccaga aatagcgcgc
    45481 tcataagtac tccttagacg atgagcggcg gcgcgttcgg acgccgcctg gatgttatta
    45541 ttgattgtct tgcttcattt tggcttttaa ttgtgccagt tgctcgctga ttgcatcatc
    45601 ggctttcagt tcggcaaact gatcgtccag cgatttttgt ttaccgaagc tgtggctttc
    45661 tgcttccgct tccatctggt caatacgacg ttcgaaagat tcgaaacgag ccattgcttc
    45721 atccagtttg ccactgtcca gctgacgacg cacatcgcgc gacgagtttg ccgcctgatg
    45781 gcgtaacatc aatgcctgct ggcgagcgcg tgtttcgctc aatttgtttt ccagctcgcc
    45841 aatctctttc ttcatgcgtg ccagcgtatc gtccaccagc gtcacttcat gttccaggga
    45901 cttaatcaga tcggtcagtt tctgtttttc aattaacgcc gcacgcgcca aatcctctct
    45961 ctctttcagc agcgccagtt cggctttttc ctgccattca acttcacgcg ccgacgcttg
    46021 ttcaatacgg cgagtcagct gtttcttttc tgccagcgca cgcgccgaag tagaacgtac
    46081 ttcaaccaat gtatcttcca tctcctggat catcagacgc accagtttct gtggatcttc
    46141 cgctttctct aacagagcgt tgatgttggc attcacgatg tcggcaaagc gagaaaaaat
    46201 acccataatg ttgtcctctt gatttctgca ttcaggattg tcctgctgaa ctgttaatac
    46261 aatttgcgtg ccaatttttt atctttttga tttataaaga tctgattgaa gaatcaacag
    46321 caacatgcca ggatgaatta gctaattaca ctaacaagtg gcgaatttca tcatggcaga
    46381 atacaaagat aatttactcg gtgaggcgaa cagctttctc gaagtgctgg aacaggtttc
    46441 gcatctcgca ccgctggaca aaccggtgct catcatcggc gaacgcggca ccggtaaaga
    46501 gctgatcgcc agccgcctgc attatctctc ctcccgttgg caagggccgt ttatttccct
    46561 taactgcgcg gcgttaaatg aaaatctgct ggattccgaa ctgtttggtc acgaagcggg
    46621 ggcgtttacc ggtgcgcaaa aacgtcatcc agggagattt gaacgtgccg atggcggtac
    46681 gctatttctt gatgaactcg ctacggcacc gatgatggtg caggagaaat tattgcgcgt
    46741 gattgagtac ggtgaactgg agcgcgttgg cggcagccag ccgttgcagg tgaatgtgcg
    46801 gttggtatgc gcgacgaatg ccgatctccc ggcgatggtc aatgaaggta cttttcgcgc
    46861 tgacctgctc gaccggctgg ctttcgatgt agtgcaactg ccgccactac gcgaacgtga
    46921 aagcgacatt atgttaatgg cggaacactt tgccatccag atgtgtcggg aaatcaagct
    46981 gcctctgttc ccggggttta cggagcgcgc cagagaaaca ttgctgaatt atcgttggcc
    47041 aggaaatatt cgtgaattga aaaacgtggt ggaacgttca gtgtatcgcc acggcaccag
    47101 cgattatccg cttgatgaca tcattattga tccctttaaa cggcgtccgc ctgaagacgc
    47161 tatcgccgtt tcagaaacca cctcgcttcc aacactgccg ctggatttac gtgagtttca
    47221 gatgcagcag gaaaaagagt tgctgcaact cagtttgcaa caagggaaat ataaccagaa
    47281 gcgcgcagct gaattgctgg gcttaaccta tcatcagttc cgcgcgttgt tgaaaaagca
    47341 ccagatttag cgaagttggg caatacaact tgccggatgc ggcgtgaacg ccttatccgg
    47401 catacataat caatacgtcg tttgcaggtc tgataagcgc agcatatcag gcattattgc
    47461 gttgcccctg cttctgcgta ttaatctctc agcgcatcct gcaaaatttt cattgccgca
    47521 tcgaattgcg catccgggat ggtcagcgga tacaggaaac gaatcacgtt gccgtatgcg
    47581 ccacaggtca gtagaagcag tccctgcgcc agcgcgcgtt gctgaatttt ttgcgcaatg
    47641 gcggctgacg gctcgcccgt ttgcgggtcg ttaaattctg ccgcaatcat cgagcccaga
    47701 ccacgtaccg ccgcgatggc cggaacgttt tctttggcat caatcagggt tttcgtcaga
    47761 cgctgcccca actgcatggc gcgctcacag agtgactctt tgtcgataat gttgagcacc
    47821 gcgtgcgcgg cagccaccgc cagcggattc ccggcgtagg tgccgccaag cccgcccggc
    47881 gcgggcgcgt ccataatatt cgcgttaccg accacacccg aaagcggcat cccgcccgcg
    47941 aggcttttcg ccatcgtcat taagtccggc ttatcggcgt aatgatccat cgcaaacagt
    48001 ttgccggtac gcgcaaagcc gctttgcact tcatcggcaa tcatcacaat accgtgctcg
    48061 tcgcacaggc ggcgaatggc ggcaaccagc tcttttggcg caacgttgaa accgccctcg
    48121 ccctgcaccg gttcgaaaat aatggccgcg acctgcttcg cttcgatgtc tgatttaaac
    48181 aagcgttcga tggcgtcgag ggagtcctgt gttgaaatgc cgtgtaaatc tgacggataa
    48241 ggtacgtgat acaccgaacc agggaacggg ccgaagccga ttttgtacgg cgcgaccttt
    48301 ccagttaacg ccatagtcat atacgtacga ccgtggaagc cgccgctaaa cgcaatcacg
    48361 ccagggcgtc cggtatgggc gcgggcgatt ttcaccgcgt tttccaccgc ttccgcaccg
    48421 gtggtgaaga acgcggtttt ggcctgcccg ctcaccgggg caagggcgtt gattttctcc
    48481 gccagggtga cgtagctttc atatggcaca atctgatacg cggtgtgggt aaactgttgc
    48541 agctgctgct ccaccgccgc gaccagatca gggtggcgat gtccggtatt cagcaccgca
    48601 atgcctgcgg cgaaatcgat gtactcgttg ccctcaacat ccttcagcgt ggcgttttca
    48661 gccgactggg cgaagaagtt acacatcacg ccaaccccgc gcggggtggc agaaagacga
    48721 cgctgatgga attcattgtt gctcatgttt cacacctttt gactcttcac tgttaatcgg
    48781 aaattaaacg cccagacgat cgcgcaggct gtaatacgcc gcgcccatcg cggtaaacgg
    48841 tacgcgcagc gtgcgcccgc cggggaacgg gtaatgcggc agattggcga aggcatcgaa
    48901 acgttcggcg tcgccgcgca gcagttcggc aatcaaacgt ccggcgagat gagtacaggt
    48961 gacgccgtgg ccgctgtaac cctgcatgta atagatgttg gtatcgaggc gaccgaactg
    49021 cggcatacgc gacagggtca gcaggaagtt gcccgtccag cgatagtcga ttttcacgcc
    49081 cttcaactgc gggaaggttt tcagcagttt cggcaccaca agacgctcaa cgtcatccgg
    49141 gtcgcgcgcg tcgtacacca cgccgccgcc gtacagcaaa cggttgtcgg cggtaagacg
    49201 gtagtaatca agcagataat tacagtcttc cacgcagtag tttttcggga tcagcgaacg
    49261 ggctaaatca tcggaaagcc gctcggtggt gatcacctgc gtaccgcacg gcatgctgcg
    49321 tttcgccagt tccggctcca ctttatcgcc caggtacgca ttcccggcga caatcacata
    49381 cttcgccgtc acctgacctg tggctgtacg cactacggcg ggcgtggtgt gctggatctg
    49441 cgtcactgcg gaaagctcat acacgcgccc gccgttcagg cggatagcgt ccgcttcgcc
    49501 aatggcgagg ttcagcgggt gaatatgccc gccgctgtga tccagcagcg caccagtgta
    49561 gcgatcgctg gcgacctcgc ggcgaatggc gttggcgtcc agcatctcca gctgtttatt
    49621 gccgtagcgt tcccagttct ctttctgctc ttcaagtgtg gcgagctgtt tatcattcat
    49681 cgccacaaac agaccgccgg ggcggtagtc gcagtcaatc tgataacgtt tgatacgttc
    49741 gcggatgatc tcaccacctt cgaacatcat gctgccgagc atacgggcgg tgtccatgcc
    49801 gtagcttttt tcgataacgt cgatgtcgcg gctgtaggag ttcacaagct gcccgccgtt
    49861 gcgcccgctg gcgccaaagc cgatgcgtga ggcttccaga accaccacgt caaagcccgc
    49921 ttccgccaga tgcagtgcgg aagacagccc ggtatagccg ccgccaacca cgcaaacgtc
    49981 gcaggaaatt gactcattca gcgtgtcgaa tggcgcgtat ttattcgcac tggcggcgta
    50041 gtaactgctg gtatgttctg tcatttcagg cctccaggct tatccagatg gttttcagtt
    50101 cggtgaattt ttcaagggca tgcagggatt tgtcgcgacc gttgccgctc tgcttatagc
    50161 cgccaaacgg cacggtcata tcgccgtcgt tgtagttatt gacgaagacg gaaccggctt
    50221 tcaggcgtcg gctcatgcgg tgcgcgcggg agaggtcgcg cgtccatacc gccgcgccaa
    50281 ggccgtactg gctgtcgttg gcaagctgta gcgcctgttc ttctgatgtg aaacgcgtga
    50341 ccaccagcac cggaccgaaa atctcttcgc ggcttaagga cgcattcggg tctacatcca
    50401 caatgatggt cgggccgatg gcgacagcca gcccggcgtt acggccatcc aacaacagtt
    50461 gccctttgct ttcgccttcc tgaataaagc tatggaccga gtcggcgtgg gcgcagtcga
    50521 ttaaggtgcc catggtggtt gcgggatcaa gtggatggcc cggctgccag ttttgcgcct
    50581 gctgttttaa cagggctaag aattcatcgg ctatgctctc ttccagcaac aggcgcgttc
    50641 cggcgatgca cacctgcccc tggttgtaga agatgcctgc tgcggtggcg cttgccgcct
    50701 gttgcaaatc cgggcagtca gcgaaaacga tgttggcgct tttgccgccc gcttccagcc
    50761 agacgcgttt catgttgctg tcgcctgcat ctttcagcag ctgtttcccg gtacgggtcg
    50821 aaccggtaaa ggcaatggcg tcgatatcgt tatgacgcga cagcgcctgc cccgcttcat
    50881 gaccaaaacc cgtcaccacg ttcaacacac catccggcaa gcctgcttct ttcgccagcc
    50941 ccgcgagacg aatcgcactg agcggtgatt tttcagacgg tttcaggaca acgctgttcc
    51001 ctgctgccag cgccgggcca agcttccagc aagtcagcag cagcgggaag ttccacggca
    51061 cgatggcggc aatcacgccg accggttcac gcacgatcat cgccagctca tggctactgg
    51121 tggtcgccac ttcgccatac actttgtcga tcgcttcggc gtaccagcga atggcgcgcg
    51181 ccgcgccggg tatatcatca cgcagactgt gacgaatcgg tttgccggtg tcgagagttt
    51241 ccagcagtgc cagctcttcg gcgttggctt ctattaaatc ggcgagttta ttcagcaccg
    51301 ctttacgttt tgccggagaa gagagtgacc agtcgccgcg ttcaaatacg ccgcgtgctg
    51361 cgctcaccgc acggtcgata tcgacgctct tgccgcgcgc aatattcgcc agcggtgcct
    51421 gggtaaccgg atcaacggtt tcaaaggttt cattttccgc cgcagcagta tattcaccgt
    51481 taataaataa gcggttttca atggcgagac ttaacgcttt atcctgccag taagccagat
    51541 gatgaaaatt cattatgact cctgtttcac gtctatcaga tatatgcagt ttgtgaacgt
    51601 cagggcgcag agatccccgg gccgtatgcc cgtttatttc tggcgacatt ctttatggat
    51661 tgccgggcgc attgcgctta cccggcttac agaacaaaaa gattaaaacg tggtgggcgt
    51721 atgggcgctg ataattcggc aaatacctgc cgacgtatta ctgaaactgt gcgggatgcc
    51781 ggtattaatg gcataacttt gccccgcgac gaggtggtaa tcctgaccat taatcgtcag
    51841 aataatttca ccttccagta cagtgcctat ttcctcaccc tgatgcttaa ttctttcccc
    51901 agtggttgtg cccggctggt acgtttcaaa gatcatcgcc agcgtgcgat tcgggttacc
    51961 gttatgaacc agcttcattg acacaccctg actgcccatc tcaattaaat cgtcctgatt
    52021 aatgacgacc tgcggctcat caggtttttc cggctcggaa aagaattccg agagtgacag
    52081 accatacacc ttcagcagct tttgcagcgt actgatggca gggctgactt tatcttgttc
    52141 tatcgtactg atagcactgt gagtcagccc ggagagttcg gcggcacgac gttgtgaaag
    52201 cccctgctgc tggcggattt ccgacaagcg ttttcctggc gccagtccct catcactcat
    52261 atttgcattt ccttaaactg tagtggtcag agtcgctgtt tttcagcgat atggtgctga
    52321 caagcggtga taaagccctc gaacaatata cgcgaaagcg cgtactcgct actgttccat
    52381 tccgggtgcc actgtacgcc cagcgcaaaa ggatgattga tgacactaac cgcctcaact
    52441 aagccatccg gcgagcgagc ttcaacacgc aaccgtgggc taacgacctt cgccccttgt
    52501 ccatgcagag agtttaccca aaagttgcta cattcaggta acaatgcaga cagcaatccc
    52561 ccctcttcaa cctgaacttc atgcgacggt gcatattgct gttccaccgg cagttcggga
    52621 tcttcccgat gttccagcaa ttcaggctgt tcgcacagct tgcgatgcaa cgaaccaccg
    52681 gttgccacca ccagttcctg taaaccccgg cagatggcga aaatggggat gcgcctttcg
    52741 agtgcggcat taattaacgc catgctcaga agatcacgct cgggatcggc gtcaggctca
    52801 tcgccgtttt caccatatag gtgcggctgc acattgctgg gactaccagg aaggtaaatg
    52861 ccatcgagtt tcggcaaaag ttgttcaagt aacgacggtt ccgccagcgc atgtggcagc
    52921 gcaattggca agccgcctgc gtggatgatg gcattcaggt acttttcttg cagagtctgg
    52981 gtcgcatgcc ccttaagcct gttcctgcac attacgacac cgataaccgg attgttcatt
    53041 atattttcca ttctcaatct cacaaagtgg actaaattat cgccattact gcggtttaaa
    53101 tgacctttat gttcaatatt ttttcaatct agcagtgggt tttcattttt gcaaactcaa
    53161 tttaacattt gacaaacatt tagtttgcat acagattcga atggtggtca ttatatttta
    53221 cgctttgata acgagcggaa aacaaaccaa aggcgaagaa tcatggaaac caatatcgtt
    53281 gaagtagaga actttgttca gcagtcagaa gagaggcggg gtagcgcctt tacgcaggaa
    53341 gtgaaacgct acctggagcg ctacccgaat acgcaatatg ttgatgtttt acttaccgat
    53401 ttgaatggtt gcttccgtgg aaaacgtatt cctgtttcaa gcctgaagaa gctcgagaaa
    53461 gggtgttatt tcccggcctc ggtatttgca atggatattc tgggcaacgt agtagaagaa
    53521 gcaggtctgg gtcaggaaat gggcgagccg gatcgtacct gtgttcctgt tctcggttcc
    53581 ttaacccctt ctgccgccga tccagagttt atcggtcaga tgctcctgac catggtcgat
    53641 gaagatggcg ctccctttga cgttgagccg cggaacgttc ttaaccgcct gtggcagcag
    53701 ctgcgccagc gcggattgtt cccggtcgta gcggtagagc tggagttcta tttactggat
    53761 cgccagcgcg acgctgaagg gtatctgcaa ccgccctgcg cgcccggcac tgatgaccgc
    53821 aatacacaaa gccaggttta ctccgttgat aacctcaacc acttcgccga cgtgctcaat
    53881 gatattgatg aactggcgca gttacagctg attccggcag atggcgcggt cgctgaggcc
    53941 tcgccgggtc agtttgaaat caacctttac catactgata acgtgctgga agcctgcgat
    54001 gatgcgctgg cactaaaacg cctggtgcgt ctgatggcag aaaagcataa gatgcacgcc
    54061 acttttatgg cgaagccgta tgaagagcac gcgggcagcg gaatgcatat ccatatcagt
    54121 atgcaaaata atcgtggcga gaacgtgctt tctgacacag aaggcgaaga ttcgccgctg
    54181 ctgaaaaaga tgctcgccgg gatgattgac ctgatgccgt catcaatggc gttgctggca
    54241 ccaaacgtga actcgtatcg ccgcttccag ccggggatgt atgtaccgac gcaggcgtcg
    54301 tggggccata ataaccgtac cgtcgccctg cgtattccat gcggcgatcg tcataatcac
    54361 cgcgtggaat atcgcgtggc gggtgccgat gccaacccat atctggtgat ggcagcgatt
    54421 tttgccggta ttttgcatgg ccttgataac gagctgccgt tgcaggaaga agtcgaaggt
    54481 aacgggctgg aacaggaagg cttacccttc ccgattcgcc agagcgatgc cctgggtgag
    54541 tttatcgaga atgatcacct gcgccgctat ttaggcgaac ggttttgcca tgtgtatcac
    54601 gcctgtaaaa atgatgagct gttgcagttt gagcgcctca tcacagaaac agaaattgag
    54661 tggatgttga aaaacgcata gtaccccttg cggggttgtt tcatcggcct gagcggagcc
    54721 aggcctgaca tttgctggta atccagacat ttcccggatg tcgcgttatg gagcgcaggc
    54781 agcaacgggc ttccgttaac gattaagact gtgacgagga aatgagatga tgccgatgat
    54841 taaatccccg catggcgcgg gaggctgcgt atgcgcccct cctgcgactg actggactcc
    54901 gccccctttg cttcccttgc taaacaggtt tgatttcagg tcgacacgac cgcaaacctt
    54961 attacgcagg ggaggcagca attatggcta ttaattcacc actgaatatt gctgcgcaac
    55021 ccggcaaaac ccgtctgcga aaatcactga aattgtggca ggttgtgatg atgggtctgg
    55081 cctatctcac gccgatgact gtgttcgata cctttggcat tgtatctggc attagcgacg
    55141 gtcacgtccc tgcgtcctat ttgctggcgc tggcgggcgt gctgtttgcc gctatcagct
    55201 acggcaaact ggttcgccag tttccggagg ccggttcggc ctatacctac gcgcaaaagt
    55261 cgattaaccc gcacgtcgga tttatggtcg gctggtcatc actgctggat tatctctttt
    55321 tgccgatgat caacgttttg ttggcgaaaa tctatctctc cgccctcttc ccggaagtgc
    55381 cgccgtgggt gtgggtggta accttcgtcg ccattttaac cgccgcgaat ctgaagagcg
    55441 tcaacctggt cgctaacttc aataccctgt ttgtactggt gcaaatctcc atcatggtgg
    55501 tgtttatctt cctggtggtt cagggactgc ataaaggaga aggcgttggc accgtctggt
    55561 cacttcagcc gtttatcagc gagaacgcgc acctgatccc gattattacc ggggcgacga
    55621 ttgtctgttt ctcgttcctc ggtttcgatg cggtgaccac actttcggaa gagacaccag
    55681 acgccgcacg cgtgatcccg aaagccatct tcctgacggc ggtctatggt ggcgttatct
    55741 ttatcgcggc gtcgttcttt atgcagctgt tcttccccga tatcagccgc tttaaagacc
    55801 cggacgccgc actgcctgaa attgcactct acgtcggcgg taagctgttc cagtcgattt
    55861 tcctctgcac cacgtttgtg aacacgttag cgtctggcct ggcctcacat gccagcgtgt
    55921 cacgtctgct gtatgtgatg gggcgcgaca atgtgtttcc ggagcgcgtg tttggctatg
    55981 tgcacccaaa atggcggact ccggcactga acgtcattat ggtcggaatt gtcgcgatgt
    56041 cggcattgtt ctttgattta gtcaccgcga cagcattgat taacttcggt gctctggtgg
    56101 cgtttacctt cgtgaatctg tcggtgttta atcatttctg gcgtaaagga atgaataaaa
    56161 gctggaagga tcacttccac tatttgctga tgccgctggt tggcgcgctg acggtgggtg
    56221 tgctgtgggt taacctcgag tcaacgtcac tgacactcgg tctggtatgg gcttcgttag
    56281 gcggcgcata tttgtggtat ttgatccgcc gctatcgcaa agtgccgctg tacgaaggtg
    56341 acagaacgcc agtgagcgaa acgtaatagt cggatgcgcg ccgcattatc ctgcctgagg
    56401 gtaatgcgcc gcgccattaa ctataaaaag atgtatgacc cggatcacga gtgctatcat
    56461 ccgcccttat gcttagcgca ttaaccccag gggcaaataa tgaaccacga tatcccgcta
    56521 aaatattttg atattgccga tgagtacgcg accgaatgcg cagagcctgt cgcagacgct
    56581 gaacgcacac cgctggccca ctacttccag ttactgctca cccgtttgat gaacaatgaa
    56641 gagatcagcg aagaagccca gcatgaaatg gctgctgaag cggggattaa tcccgtacgc
    56701 attgatgaga tcgcagagtt tttgaatcaa tggggtaatg agtaattatt taattgcaga
    56761 cgatcggtca gtatcgttgt ccggataaag cgttcacgcc gcatccggct aacaatgaca
    56821 cgcactgcag cgtgctttaa tgggtttctc acgatcgtac cgctctgctc ctcgctaagt
    56881 tgttctgtga gttatagtta tagagaaatc acgggtacga atgttgaaca gatggcgacg
    56941 ccatctgttc aacgaccaac cacacggccc tcattacgac ccctgaacac aaacggtaat
    57001 ggaaaatgtc attaaatatt agtaagatag cctctttaca taaaatccct taatattatg
    57061 cgccaggtat tatcgtctct tttggtgatt gctggacttg tgagtggtca ggcaatcgcc
    57121 gcgcctgaat cccccccgca tgctgatatc cgcgacagcg gttttgtcta ttgcgtcagc
    57181 gggcaagtca acacctttaa cccatccaaa gcgagcagtg ggttaattgt cgataccctt
    57241 gccgcccagt tttatgatcg actgctggat gtcgatccct atacctatcg cctgatgcca
    57301 gaacttgccg aaagctggga agtactcgac aacggcgcga cctatcgctt ccacctgcgt
    57361 cgcgatgtcc cgtttcaaaa aaccgcctgg tttactccca ctcgcaaaat gaatgccgac
    57421 gatgtggtgt ttaccttcca gcgaattttt gaccgcaaca acccgtggca taacgtcaac
    57481 ggcagcaact tcccctactt cgacagcctg caatttgccg ataacgtgaa aagcgtccgt
    57541 aaactggata atcataccgt tgagttccgt ctggctcagc cggatgcttc ttttttgtgg
    57601 cacctcgcaa cccattatgc ttcggtcatg tcggcagaat atgcccggaa gttagagaaa
    57661 gaagatcgcc aggagcaact cgaccgtcaa ccggtcggca ctggaccgta tcagttgtcg
    57721 gaataccgcg ccgggcaatt tattcgccta caacgtcatg atgacttctg gcgcggtaaa
    57781 ccgttaatgc cgcaggtggt ggtggattta ggctccggcg gcaccggacg tctgtcgaaa
    57841 ctcctgaccg gggaatgcga cgttctggcc tggcctgctg ccagccagct atccattttg
    57901 cgtgacgacc cgcgcttgcg tttaacgctg cgtcctggga tgaacgtcgc ctatctggca
    57961 tttaacaccg ccaaaccgcc gctaaataat cccgctgtcc gccatgcgct ggcactggcg
    58021 attaataacc agcgcctgat gcaatccatc tattatggta cggctgaaac ggcggcctct
    58081 attttaccgc gcgcctcgtg ggcctatgac aacgaggcta aaattactga atacaatccg
    58141 gcgaaatcgc gcgaacagtt gaagttgttg gggctggaaa atttaacgct gaaactgtgg
    58201 gtgcccacac gttcgcaggc gtggaacccc agtccactga aaactgccga actgattcag
    58261 gcggatatgg cgcaggttgg cgtaaaagtg gtgattgtgc cggtagaagg tcgctttcag
    58321 gaggcgcggt tgatggatat gagccatgat ctgacgttat ccggttgggc gacggacagt
    58381 aacgacccgg acagtttctt ccgtccgtta ctgagctgcg cggcaattca ttcacagacc
    58441 aacctcgccc actggtgcga tccgaaattc gacagcgtgt tgcgtaagac gctctcctcg
    58501 cagcagctgg cggcgcgtat tgaagcctat gacgaagcgc agagtattct ggcgcaggaa
    58561 ttgcccattt tgccgctggc gtcgtcattg cgtttgcagg cctatcggta cgatatcaaa
    58621 ggtctggtac ttagcccgtt tggtaacgcc tcctttgctg gggtgtatcg cgagaaacag
    58681 gatgaggtga aaaaaccatg attatcttca ccttacgccg cattttgtta ttgattgtca
    58741 ccttgttcct gctgaccttc gttggcttca gcttgagcta tttcactcca cacgcgccgt
    58801 tacaaggcgc gtcactgtgg aatgcctggg tgttctggtt taacggcctg atccactggg
    58861 attttggtgt ttccagcatt aacggtcaac caattgccga acagttaaaa gaggtcttcc
    58921 cggcgacgat ggaactgtgc atcctcgcct tcggttttgc gctgattgtt ggcatccccg
    58981 tggggatgat tgccgggatt acgcgccata agtggcagga taacttgatc aacgccatcg
    59041 ccttactggg cttttcaatc ccggtgttct ggctggcgct tctgttgaca ctgttttgtt
    59101 cactcacgct aggttggttg cccgtttcag ggcgtttcga tctgctctac gaagtgaaac
    59161 cgattaccgg ttttgcgttg attgatgcct ggctttcgga ctcaccatgg cgggatgaaa
    59221 tgatcatgag cgcaatccgc catatgatat tgcccgtgat caccctgtcg gttgcgccaa
    59281 caactgaagt gatccgcctg atgcgtatca gcactatcga agtgtatgac cagaactatg
    59341 taaaagcggc ggcgacccgc ggtttgtcgc gctttactat tttgcgtcgc cacgttctgc
    59401 ataacgcgtt accgccggtt attcctcgcc tgggcttaca gttttccacc atgctgacgc
    59461 tggcgatgat caccgaaatg gtttttagct ggccgggttt agggcgctgg ttaattaacg
    59521 ccatccgcca gcaggattac gcagccattt ccgccggagt gatggtgtgt ggctcactgg
    59581 tcattattgt taacgtgatt tctgatattt tgggtgccat ggctaaccct ctgaaacata
    59641 aggaatggta tgccttacga tagcgtatac agcgaaaagc gcccgccggg cacgctgcgt
    59701 accgcctggc gcaaatttta tagtgacgcc tctgcgatgg tcggcctgta cggctgcgcg
    59761 gggctggctg tactgtgtat gtttggcggc tggtttgcgc cttacggcat cgaccagcaa
    59821 tttctcggtt accaattatt gccgccatca tggtcgcgct atggcgaagt ttctttcttc
    59881 ctggggactg acgacctcgg acgcgatgtt ttaagccgtt tactgagcgg agctgcgcca
    59941 accgtgggtg gcgcatttgt ggtgacactt gccgcgacga tctgtggcct ggtactgggc
    60001 accttcgccg gggcgacgca cggcctccgc tcagcggtgc ttaaccatat tctggatacc
    60061 ttactggcga tcccttccct gctgttggca attatcgttg tagcgtttgc cggaccgagt
    60121 ttgtcccacg ctatgtttgc tgtctggctg gcgctgctgc cgcgtatggt acgttcgatt
    60181 tacagcatgg tgcatgacga actggaaaaa gagtacgtta tcgccgcccg tctggatggc
    60241 gcatcaacgc tgaatattct ctggtttgct gtgatgccta acatcaccgc cgggctggtc
    60301 accgagatca cccgcgcact gtcgatggca attctcgata tcgccgcgct gggctttctc
    60361 gatcttggcg cacaactccc ctcgcctgaa tggggagcga tgctcggtga tgcgctggaa
    60421 ctgatttatg tcgccccgtg gactgtcatg ctgccaggtg cggcaattat gattagcgtg
    60481 ctgttggtta acctccttgg cgacggtgtc cgtcgtgcaa ttattgcggg ggtggaataa
    60541 tgccattact cgatattcgt aacctgacta ttgaatttaa aaccggtgat gagtgggtta
    60601 aagccgttga ccgggtaagt atgaccttaa ccgaaggtga aatccgcggt cttgttggtg
    60661 aatccggttc cggcaaaagt ttgattgcga aagcgatttg tggggtgaat aaagataact
    60721 ggcgtgttac tgcagaccgt atgcgttttg atgatatcga tttgctgcgt ctctccgcac
    60781 gcgaacggcg caaactggtt ggccacaacg tgtcgatgat tttccaggaa ccgcagtcgt
    60841 gtctagatcc ttcagaacgt gtgggtcgcc agttgatgca aaacatccca gcctggacct
    60901 ataaaggccg ttggtggcag cgttttggct ggcgcaaacg ccgtgccatt gaactgctgc
    60961 accgcgtggg aattaaagat cataaagatg cgatgcgcag ttttccctat gagttgaccg
    61021 aaggtgaatg tcagaaagtg atgatagcca ttgcactggc gaatcaaccg cgtctgctga
    61081 ttgctgacga accgacaaac tcaatggagc caacaaccca ggcgcaaatc tttcgcctgc
    61141 tgacgcgtct aaaccaaaac agcaatacca ctattttgct tatcagccat gacttacaaa
    61201 tgcttagcca atgggcggat aaaattaacg tgctttactg cggtcaaacg gtggaaaccg
    61261 cgccgagtaa ggagttagtg acgatgccac atcatcctta tacccaggcg ctgatccgcg
    61321 cgataccaga cttcggaagc gcgatgccgc ataaaagtcg cctcaatacg ctacccggcg
    61381 ctatcccact gcttgaacag ttaccgattg gttgtcgtct ggggccacgt tgcccgtatg
    61441 cacaacgaga atgcattgtg acgccacgtt tgacgggggc gaaaaatcat ctctatgcct
    61501 gtcatttccc gctgaacatg gagaaagagt gagatgatcg aaacgctgct tgaagtgcgt
    61561 aatttaagta aaacgttccg ctaccggacc ggttggtttc gtcgtcagac cgtagaagcg
    61621 gtaaaaccct tgagctttac gctacgtgaa ggccagacac tggcgattat tggcgagaat
    61681 ggttcgggta aatccacgct ggcaaaaatg ctggcgggaa tgatagaacc taccagcggc
    61741 gagttattga ttgacgatca tccactgcat tttggcgatt attccttccg cagtcagcgt
    61801 attcgcatga tttttcagga tccttcgacc tcgttgaatc cccgtcaacg tatctcgcaa
    61861 attctggatt ttccactgcg cctgaacacc aacctggaac cggaacagcg tcgcaaacag
    61921 attattgaaa cgatgcgtat ggttgggctg ctaccggatc acgtcagtta ctatccgcat
    61981 atgttggccc ccgggcaaaa acagcgtctg ggtctggcgc gcgcgctgat attgcgccca
    62041 aaagtcatta ttgccgatga agccctcgct tcactggata tgtcgatgcg ttcgcagttg
    62101 attaatctga tgctggaatt acaggaaaaa cagggcattt cgtatattta tgttacccag
    62161 catatcggaa tgatgaagca cattagcgac caggtgctgg tgatgcatca gggcgaggtt
    62221 gtcgagcgag gcagcaccgc ggatgtgctg gcatcgccgc tgcatgaact caccaaacgg
    62281 ctgattgccg ggcactttgg cgaagcattg acggcggatg cgtggcgtaa ggaccgttag
    62341 tgttctgcac ctcatcctga caggggaccc cccctcagga tgaggtattc attacttttg
    62401 attgtcgatt ctggtgagag caaccaggaa tgccagtgta taacacaccg taattgccag
    62461 actcattttt aacagtgttt ctccgccaaa tcccgacaat ggcgcggcta taccgccgaa
    62521 gacaaacatg ctcatcccca tcaacgcaga cgccgttcca gattcctgcg tccctactgc
    62581 actcatagcc tctgccccgc caacagtgcc gatgccgcta caaaatgcaa tagtgaaaaa
    62641 taatgccacc agtgccggaa tgggcaattg tgtccatgcg cataagaccg tgagcaatgc
    62701 acacaaaatt gccgctatca ggccacctcg caacaatgtc atcgcattaa tccgtcgcgc
    62761 cagacgcgaa aagatccatg aagcaatgat aagtccgatg ccgttaaggc caaacaccag
    62821 gctaaattgc attggactaa agccaaattc cttctgcaac acgaacgacg aagagccgat
    62881 atacgcaaac aagcctgcca gcataaaact ttgaatcagg caaaagcgca taaaacggcg
    62941 gttctgcacc acgcttcgtc cggttaatag caatgatgag ccccttttat tttctggcaa
    63001 ggtctcatta ataaataaca cgcagcccag caacaatacg gtgctaattt cagccattac
    63061 ccagaataaa gtgcgccagt caaaagtgct aacaatgtac ccgcccagca ctggcgacaa
    63121 caccggtgcc aggccattca ccgtcattaa cagcgcaaaa aactgggtca acgttactcc
    63181 ctgatatttg tcacgagcaa tagaacgaga gagcaccgaa ccacccgccc ccgcgatccc
    63241 ttgaataaaa cgccagacca ccagccagta aatattgttc gttgtcgcgc acaaaatgga
    63301 agaaacaata aacaatagca acgacaagat taacggacgt ttacgcccta ttttgtcgct
    63361 cagagggcca aataacagtt gtccgacgcc aagaccgatc agtgatgcag tcagcgttaa
    63421 ttgcgttatg gtcgtggttg ccgccagttg ctcagacatc tccggcaaag ccggcaaata
    63481 aagatcggta cacatcgggc cgataccggc taaaagacca agaatcaatg cccatgaaag
    63541 agagactcta gccatattat tccttacttg agtgatgaca tgccgccgcc cagcgactgc
    63601 cagagcgtga ttcggtttgt aaaatcagtc agttgcagtg ccagcagctc ctgctgtgcg
    63661 ctccacaagc tgcgctgcgc cgtcaacacg gtcaggtaat caccaacacc cgtctgatag
    63721 cgacgcaagc caacatcgac cgtttgttgc tcagctttaa catactggcg ctgtgcatcc
    63781 agttgctctt caagcgtggt ccgccgcgcc agtgcgttag cgacatcttt aaatgctctc
    63841 tgaacggttt tttcataggt ggcaatgagt ccacgctttt gtgcttttgt gcttctgcgt
    63901 aacgaagctg cgccagatta cttccgccag tgaacagcgg taatgtgacc gatggcgtaa
    63961 atgaccagat ctgcattccg tggctgaaca acgacgacaa tgcgtcactg ccaacaccag
    64021 cgctggcagt cagcgaaatc gtcgggaaaa agttagcccg cgccgcgcca atatcggcat
    64081 tggcgctctt caaattatgt tccgcttcct ggatatccgg gcgacgcagc agaacgtcag
    64141 atgacactcc cgcaggaacc agacttatca tctgttccgg caggctttcc agtgttcccg
    64201 gtagcaaatt ttcctcaagc gttgtacctg ccagtaagtt cagcgcattt ttatcctgca
    64261 tcacttgtgt ctgatagctg gcaacgctgg cgcgcgcttg ctgataaaca ctcatcgcct
    64321 cactgacatc tgtcgctgcc gccgtgccaa cctgttgctg gcgctggata attttcaatg
    64381 agttttctgc actggtcatc gtttcttttg ccagggccag gttgctgtta tcggccgcca
    64441 gtgtcagcca ggcggtgctg atttcagcaa tcagcgttaa acgggtgttc tgggctgtaa
    64501 attcactggc aagccaggtt tctcgcgccg cccgagataa actctgatta cgaccaaaca
    64561 gatctaacgt aaaactggac accgtgccgt cagcctcggc agtggtttcc gtaccattcg
    64621 ccagcgaacg actgcgggtt gaacttaagg ccgcattcac cgtcgggaat aacgacgcat
    64681 tagtttgccc atagagcgcc cgtgctgagt caatatcagc aatcgctttt tgcacatcgc
    64741 ggttactgtt cagtgcaata gtcaccacct gctgcaatcg gggatcgtgg atcacctgct
    64801 gccagtcatg gctgattgcc tttccctggc cctgtgctcc cggcaaggtc gccgggattg
    64861 gggattcagg cgtgctgtag tggggatcga gcgagacaca tcccgcactc aacagcacca
    64921 gaaaaataag agaacgacgc aacataactt actcctgacg gcgcgattta ccggcaaaca
    64981 aacgcttcac cagtacaaaa aacagaggaa caaagaaaat agccagcaac gtagcggtca
    65041 gcgtaccgcc aataatgccc gtaccaatgg cgatgcggct gttcgcccct gagccggttg
    65101 ctatcgccag cggcattacc ccccgcaata aacgccagcg aggtcatgat gattgggcgt
    65161 aaacgagtct gcgccgcgcg taatgccgca cgactcaagg aataaccctc tgcaaccgcc
    65221 gcttcagcaa attccacaat cagaatggcg tttttcgacg acaagccgat agtggttaat
    65281 agcgccacct ggaagtaaac atcgttgttt aaatcacgca tccaggccgc cagcgccgcg
    65341 ccaagaagcc ccagagggat caccagaatg accgagaacg ggactgacca gctttcgtac
    65401 agtgctgcaa ggcagaggaa aaccaccaga atagaaactg catacagact caatgcctga
    65461 ccgctggcca gtttctcctg caatgacaaa ccactccagg cccaggttgt tcccgcagga
    65521 agactgtttg ccagttcctc catttttgtc attgcatcgc cggaactaaa gccagtcgca
    65581 ttttccccct gaatttcata ggctgcagaa ccgttgtagc gcaccagtct ttccggtcca
    65641 tacagccagc gggtggtcgc aaaagcagag aatggtgtca tggcgttatc actaccgcgc
    65701 acaaaccatt tacctaagtc tgacggcgcg gaacgaaatt cactgtcgcc ctgaatgtag
    65761 acttttttca cacgaccacg gtcaataaag tcattcacat aagtaccgcc ccacgcactg
    65821 gacagggtgt cggtgacatc attcaaactc aaccctaatg acaccgcttt attactatca
    65881 atatctacct gcaattgcgg catttgtggc aaatcattgg cacgaacaga atgtaactcc
    65941 ggactttgat tcgctttttc tatcaactga ttacgcattt gcagtagtgt ttcgcgatcg
    66001 gttccaccgt tagctaacaa ttcaaacgta aaaccattgc tttgccccag cccatcaacg
    66061 gctggcggcg tcatcgcaaa taccgtggca tcacgaattg tgcccagctc tttggttgcc
    66121 cgtagggcga tagcctgggc ggtgttttct gcccctttac gttgagacca gtttttcaac
    66181 gaaacaaacg ccatcccggt gttctgtccg ctgccgctaa aactgaaacc atcaacggta
    66241 aaaatgacat cggtatttgc tttctcgtta atcaggaacc agtcaacaat ctggcgattc
    66301 acttctgctg tacgggcagc ggtcgccccg gccggcagcg tgtactgcac catgatttcg
    66361 ccctggtctt cagtgggtaa gaaactgccc ggcagtttcc acatcatcag ggccattcca
    66421 ccacccagta cgagataaag ccccatcgtt cgagcagcac ggcgcaggac ataaattacg
    66481 cctcgctgat atttatcttc agtacggcgg taaaagcggt caaatgcgcc gaaaaagcct
    66541 tttttatgtg gcggaacatg ctggaggacg gaaccgcaca gggcgggagt caaggtcaat
    66601 gccaccacca cggaaagcag cattgcggag ataatggtta tcgaaaactg acgataaatt
    66661 acccccgtag atcccccaaa gaaggccata ggcaggaata cggctgacaa caccagcgca
    66721 atggcaacca gtgcgccaga aatctcgcac attgattttt ccgtggcttc acgcgcgggc
    66781 aaccctttgt cacgcataat gcgttcgacg ttctccacta ccacaatggc atcgtccacc
    66841 agcaagccga tcgccagcac catagcaaac aacgtcaggg tattaatcga atagccaaac
    66901 aacgcaagaa cgccgaatgt gcccagcaaa acgacgggga cagctaacgc cggaatcagt
    66961 gtggcacgca ggttttgcag gaataaatac atcacgcaaa ccaccaatac gatggcttca
    67021 aacagcgtct gaattacgtc ctctacagag attttaataa attcagtgct gtctttcggg
    67081 taagcgatgt cgtatccctg cggcatgttc cgctggaatt cggcaatctt atccttgacc
    67141 agcgtcgccg tattcagcgc attcgcaccg ggcgaaagca ttacagcaac cccggcagac
    67201 gggtgaccgt taagtttgcc gatagcggta taatcttcac tgcccatctc aacgcgagcc
    67261 acatctttta tacgaacaac tgcgctgtct gactggcttt tcacgataat atttttgaac
    67321 tgttccaccg tctgcaagcg agactgcgca cgaaccgttg cggtcagttg ctgagtattc
    67381 ggtgaaggca atgcccctat tttcccggca gtaacctgca cgttttgcgc ttcaatagca
    67441 ctttgcacgt ctgaaggcat tagcgagtaa gacgccagtt tggccgggtc aagccagatg
    67501 cgcatagcgt attccgcgcc aaagacttgc agactcccca caccttcaac acgcgccagc
    67561 gggtcctgaa cgttactaac caaccagtcg gcgatatccg aactggaggc tttgtcagtg
    67621 gtgtcataca cggcagcaat cagcaaaaag ttgctttgtg atttctccac cgtaacaccc
    67681 gtttgctgca cttcgctggg taggcgcgac tccgcctgct gaattttatt ctgcacctga
    67741 acctgtgcag tatctggatc ggtaccttgt tcaaaggtca cattaatact gaccgaacca
    67801 tcagagctgc tggttgagct gaaatagagt aaattatcaa gcccagtgag ttgttgctcg
    67861 atgacctgag tcacgctgtt ctccagcgtt tcggcagaag caccagtata agtggctgaa
    67921 attttaatgg tcggtggcgc aacgtcagga tattgcgcga cgggcaatgt gcgaatagcc
    67981 agaatccccg ccaacataat caaaatggcg ataacccagg caaagaccgg gcggcgaaca
    68041 aaaaagcgcg aaaacatcag gcgttgcctc cattagcctg aacttcaaca gccttgaccg
    68101 tctgccctga agtgactttg gcagaacctt caacaatcag tcggtcgccg ctgtgcaggc
    68161 cgtttagcac cagccattta tcaccatatg tttctcccgt ttcgagcgtt cgctgctcta
    68221 ctttattgtc tttattcacc accagcgcag ttgcattgcc tttagcatcg cgcgtgacgc
    68281 cctgttgcgg cgcgagaata gcgtcttcca tcacgccttc atcgacacga gcgcgaacaa
    68341 acattcccgg taataattgc tgttgtggat tggggaaaat cgcccgtaat gtcaccgaac
    68401 cggtagactc atctacagcg acttcggtga gttccagacg ccctttttcg ctgtaggttg
    68461 tgccatcttc cagaataagt gagacgctca tggtgtcact gttggtcgcc agtgactgtt
    68521 tacgcagacg taataaatcg acactggagc gagtgaggtc gacatacatt gtatccagac
    68581 cacgaatagt cgttaacgct gtatcttgcg acgcggtcac cagtgcgcca ggggttaccg
    68641 acgaaatgcc aatgcgccct gaaatcggtg cggttaccgt ggtccagtca agattaatgc
    68701 gcgcagtttc cagtgcggct tttttcgccg ctacactggc tttatcttgt gcacaggtag
    68761 actgagcatc atcagcatcc tgttgtgaaa caccgttctc tttcaccagt cgggcataac
    68821 gctgcgcttt ctggcaatcg gcttttacca gtgcctgtgc ttgttgtaat gctgcccgag
    68881 cttcattcca tgcagcctga taactggccg catcaatctg gtagagcggc tgtccagcct
    68941 tgaccagatc accttcctta aataagcgtt tctggataat tcccccaacc tgcggacgta
    69001 cttcggcact gagcgcagca ctggtgcgtc cggttaattc actgactacc gagaccggtt
    69061 gagatttgac tgtaacaacg ccgacttctg tttctgacgg ggatgaattg ttactttgcg
    69121 tattatcaca accagataag agcagcattg ccactacaga tgttgctata tacttcatta
    69181 ttttctccag ggtgagaaat accctcctca ttaaaattaa tgaggatatg tcagggatat
    69241 tcagttataa cgaacccgca tcgcgaaatg aatttcgcaa tgaaataagg gggattaata
    69301 tcccccgcta ttggttaatt ctgttcttat ttatttatca acatactaac aatatcctga
    69361 tatatttcct gcagatgttg tggatcgctt ttttgcggtg ttaaacgacg ataaaccgta
    69421 ccttccatca tggtagcaaa cacttcaacg cagcaacgaa tgtgctcctc gctaagatgg
    69481 ggatgcattc ttttcatatg tgcacaaccg ttagcgaaca ttctggcgtc agcttcttca
    69541 agcattgcca taacccgcgg gttgcgtgtg gcttctgcag ccacttccag catcagcgcg
    69601 tcgtcgtcct catttaagga tttacgcaag gcaaggactt ccgggaagtg atcggtacgc
    69661 gcgtcaatgt ccatttgggc aatacgaaag tcgatgatac ggcgaaccat ctcttcaata
    69721 atggcgtctt tgttggcgaa gtaacgatat atctgaccca cgctaagctg agcttcgctg
    69781 gcaatttgcg acatactggc ggcatgaaac ccgcagcgac gaaaacaccg acgggcggcg
    69841 ttaatgattt catcctgacg ctgtttgtgg cgtatttcca gcttgctggt catttctgtc
    69901 tcctggccta tcacttggtg agaatgatcg ttctcatttt tgctgattat acgtttctcc
    69961 aaagtaatgg aaagtatttt tatcttattc atggtgcata ttctgataat gaagagattg
    70021 tggagattct gtgaagatag ttgacaatat taagttgggg tcaattcatc agaaaggctg
    70081 ctacttgttg tcgccagttc tgttttcatc atcaaactta tcggagctaa tacgaatagt
    70141 ttcatagtca gattctataa gatatttccc caacctaccc cttgcaggcg tcaagagtag
    70201 ataacgcaaa atgctctgta ccgcagcttc tctccggtac agaaagcgca actatagcca
    70261 cccacagcca ggttgattat aataaccgtt tatctgttcg tactgtttac taaaacgacg
    70321 aatcgcctga ttttcaggca caacaagcat caacaataag gattaaagct atgggttttc
    70381 tttccggtaa gcgcattctg gtaaccggtg ttgccagcaa actgtccatc gcctacggta
    70441 tcgctcaggc gatgcaccgc gaaggagctg aactggcatt cacctaccag aacgacaaac
    70501 tgaaaggccg cgtagaagaa tttgccgctc aattgggttc tgacatcgtt ctgcaatgcg
    70561 atgttgccga agatgccagc atcgacacca tgttcgctga actggggaaa gtttggccga
    70621 aatttgacgg tttcgtacac tctattggtt ttgcacctgg cgatcagctg gatggtgact
    70681 atgttaacgc cgttactcgt gaaggcttca aaattgccca cgacatcagc tcctacagct
    70741 tcgttgcaat ggcaaaagct tgccgctcca tgctgaatcc gggttctgcc ctgctgaccc
    70801 tttcctacct tggcgctgag cgcgctatcc cgaactacaa cgttatgggt ctggcaaaag
    70861 cgtctctgga agcgaacgtg cgctatatgg cgaacgcgat gggtccggaa ggtgtgcgtg
    70921 ttaacgccat ctctgctggt ccgatccgta ctctggcggc ctccggtatc aaagacttcc
    70981 gcaaaatgct ggctcattgc gaagccgtta ccccgattcg ccgtaccgtt actattgaag
    71041 atgtgggtaa ctctgcggca ttcctgtgct ccgatctctc tgccggtatc tccggtgaag
    71101 tggtccacgt tgacggcggt ttcagcattg ctgcaatgaa cgaactcgaa ctgaaataat
    71161 cgttctgttg gtaaagatgg gcggcgttct gccgcccgtt atctctgtta tacctttctg
    71221 atatttgtta tcgccgatcc gtctttctcc ccttcccgcc ttgcgtcagg ataacgattt
    71281 cctttacgac caaggagcgc ccatggaaca acgccacatc accggcaaaa gccactggta
    71341 tcatgaaacg caatccagta ctacggagta tgacgttctg cctctggtcc cggaagccgc
    71401 aaaggtcagc gatccctttc tactcgacgt gatccttgaa aaagaaacgc tggccccctt
    71461 cctttcatgg ctggaccctg cgcgtgttct tgcagtggag ttgttccctg accagcttac
    71521 cgtgacccgt tcacagacct tcaccgctta tgaacgcttg tcgacggccc tgacggttgc
    71581 tcaggtttgc ggcgtccagc ggttatgtaa ctactattcg gcgcgactta cgccgctccc
    71641 cgggcctgat tccaccaggg aaagtaatca tcggttggca caaatcacgc aatatgcccg
    71701 ccaactggct agctcgcctt ctattatcga caaccgatcg cgccagcatc tgaatgacgt
    71761 cggtcttact gcctgggact gtgtgatcat taaccaaatc attggtttta ttggctttca
    71821 ggcgcggaca attgcgacat ttcaggctta tctcgggcat ccggtacgct ggttacccgg
    71881 gctggagata caaaactacg ccgacgcgtc actgtttgct gatgaatcat tacgctggcg
    71941 aagcagctat gaggtggaaa aactacctga agaacacaca aaaagttcaa ctgcagaact
    72001 ttgccaactg gccgaaatac tctctctcca ccctatttca ctttcccttc tcgaaaggtt
    72061 gttaaacagc acacgggtta atacacagcc ggataatcag cttgcggcgt tgttatgcgc
    72121 gcggataaat ggcagtcctg cttgttttgc cgcctgtatg gattcatcaa atgaatataa
    72181 aaaaatcagc ccccttctgc gcaagggcga aaatgaaatt aaccaatggg ctgaccgtca
    72241 ttctgttgag cgcgctaccg ttcaggcgat acaatggctg acccgagcac ccgatcgctt
    72301 tagcgccgcc cagttcagcc ctttactcga acacgaaaaa tcatcaacgc agattattaa
    72361 tctgctggta tggagcgggc tgtgtggctg gataaatcgc ttaaaaatcg cgttgggtga
    72421 gacatattaa ccttgccgcg tcagacagat tcgcgtaaaa ctgtcagccg ctctaatggc
    72481 caccaaaata gacaattatg tttcaggaca acccgctgct agcgcagctt aaacagcaac
    72541 tgcattccca gacgccacgc gctgaagggg tggtaaaagc cacagaaaaa ggttttggct
    72601 tcctggaagt cgacgcgcaa aaaagttatt tcattccgcc gccgcagatg aaaaaagtca
    72661 tgcatggcga ccgaattatc gcggtgatcc acagtgaaaa agaacgtgaa tccgcagagc
    72721 cagaagaact ggttgaaccg tttctgactc gtttcgtggg taaagttcag ggcaaaaatg
    72781 accgtctggc catcgttcct gatcatccac tcttaaaaga cgccattcct tgccgcgcag
    72841 cccgtggtct gaaccacgag tttaaagaag gcgactgggc agttgccgaa atgcgccgtc
    72901 atccgctgaa aggcgatcgt tctttctatg ctgaactgac acaatacatc acttttggtg
    72961 acgaccactt tgtaccgtgg tgggttaccc ttgcgcgcca taatctggaa aaagaagcac
    73021 cagacggcgt cgctaccgaa atgctcgatg aaggtctggt tcgtaaagat ctgaccgcgc
    73081 tggattttgt caccatcgac agtgccagca cagaagatat ggatgacgcc cttttcgcta
    73141 aggcgttgcc ggatgacaaa cttcagctga ttgtggcgat tgccgatcca accgcgtgga
    73201 ttgctgaagg cagcaagctg gacaaagccg cgaaaattcg cgcattcacc aactatctgc
    73261 ctggcttcaa catccctatg ctgcctcgcg agctttctga cgatctctgc tcactgcgcg
    73321 ccaatgaagt ccgcccggta ctggcatgcc gcatgacgct ctccgctgat ggcaccattg
    73381 aagataatat cgaattcttt gccgccacca tcgaatccaa agcgaagctg gtgtatgacc
    73441 aggtttctga ctggctggag aataccggtg actggcagcc tgaaagtgaa gcaattgccg
    73501 aacaagtccg tttgctagcg caaatttgcc aacgccgcgg cgagtggcgt cataaccacg
    73561 cactggtgtt taaagatcgc ccggattacc gctttattct cggtgaaaaa ggtgaagtgc
    73621 tggatatcgt cgccgagcct cgtcgcattg ccaaccgtat cgtcgaagaa gcgatgattg
    73681 ccgctaacat ttgtgcagct cgcgtactgc gcgataagct cggttttggt atctataacg
    73741 tgcatatggg ctttgatccg gcgaatgccg acgcgctggc agcgttgctg aaaacgcacg
    73801 gtctgcatgt cgatgccgaa gaagtgctca cgctggacgg tttctgcaaa ctgcgtcgtg
    73861 aactggatgc gcaaccaact ggtttcctcg acagccgcat tcgtcgcttc cagtcatttg
    73921 ctgaaattag cactgaaccc ggtcctcact ttggcctcgg tctggaagca tacgccacct
    73981 ggacttcgcc gatccgtaaa tatggcgaca tgatcaacca ccgtctgctg aaagcggtta
    74041 tcaaaggcga aactgcgacg cgtccacagg atgaaatcac tgtccaaatg gccgagcgtc
    74101 gccgtctcaa tcggatggca gaacgtgatg ttggtgactg gttatacgca cgcttcctga
    74161 aagacaaagc cgggaccgac acccgtttcg cagcggaaat tgtcgatatc agccgtggcg
    74221 gcatgcgtgt tcgtttggtt gataacggcg ctatcgcctt tattcctgca cctttcttac
    74281 acgctgtgcg cgatgaactg gtttgcagcc aggaaaacgg caccgtacaa attaaaggtg
    74341 aaacggttta taaagtaact gacgttattg acgtcaccat tgccgaagtc cgcatggaaa
    74401 cccgcagcat tattgcgcgc ccggtcgcgt aatctccttt cacggcccat tccttatgaa
    74461 tgggccgttt atttccccgc tctaccttca tcatatcccg gcagtttttt aactgacttt
    74521 cgtttgaaaa ctggcgtttt tccaaaacta gattacaaat aaatatataa aaaaaacaat
    74581 gtgtttagcc gccgttaatt tcatgtaagt catgttgttc cttttattta accgcggact
    74641 ccgctgttaa ccggaggata tgcatcatga aaaccgttag ggagtccaca acgttgtaca
    74701 actttctcgg atcgcacaat ccatactggc ggttgacgga aagcagcgat gttttgcgct
    74761 tttctaccac cgaaaccaca gaacctgaac gtacgttgca gttatctgcc gaacaggctg
    74821 ctcgcatcag ggaaatgacg gtcatcacct ccagcctgat gatgagtctg accgtcgatg
    74881 agagcgatct ttctgtgcat ctggtaggac gaaaaatcaa taaacgggaa tgggctggca
    74941 acgcgtctgc atggcatgac acaccagcag ttgctcgtga tttatcacac ggactttcct
    75001 ttgctgagca ggtagtttct gaagcacatt ccgcaatagt gattctcgac agccggggga
    75061 atatccaacg cttcaatcgg ttatgtgaag attacacagg gttgaacgaa cacgacgtca
    75121 ttggccaaag cgtgtttaaa ctgtttatga gccgtcgtga agctgcggca tccaggcgca
    75181 ataaccgtgt attttttcga agcggcaatg catatgaagt cgaactgtgg ataccaacac
    75241 gtaaaggcca gcggctgttt ctgtttcgca ataaatttgt ccacagcggc agtggcaaaa
    75301 acgagatttt tttaatctgt tccggcaccg acattaccga agagcgccgc gctcaggaac
    75361 gactgcgtat tctggcaaat accgacagta tcaccggact gccgaatcgt aacgcaatgc
    75421 aggagttaat cgatcacgct attaatcagg cagataacaa taaagttggg gtcgtgtatc
    75481 ttgatttgga taattttaaa aaggtcaacg acgcctatgg gcatttattt ggtgaccagt
    75541 tattacgcga cgtgtcattg gctattttaa gctgtctcga acatgaccag gtgttggcgc
    75601 gtccaggtgg cgatgagttt ctggtactgg catccaacac ctcacaaagc gcgctggaag
    75661 caatggcatc acgaattttg acccgcttac ggctcccctt tcgcattggt ttaattgaag
    75721 tttataccag ctgttcagta ggtattgcac tctctcccga acatggttcg gacagcgcgg
    75781 ctattattcg tcacgccgac acagcaatgt acacagcaaa ggaaggcgga cgaggacaat
    75841 tttgcgtttt taccccagaa atgaatcaac gggtatttga atatctctgg ctggatacca
    75901 acttgcgtaa agcactggaa aacgatcagt tggttattca ctatcaaccc aaaatcacct
    75961 ggcgtggcga agtgcgcagt ctggaagcac tagtacgttg gcagtcacct gaacgtgggt
    76021 tgattccacc gttggacttc atttcctacg cagaagagtc agggctaatt gtgcctttag
    76081 gccgttgggt gattctcgat gtcgtacgcc aggtggcgaa gtggcgggat aaaggcatta
    76141 acctgcgagt ggcggtaaac atttctgcac gtcagctcgc cgatcaaacc attttcaccg
    76201 ccctgaaaca ggttctccag gaactcaatt ttgaatactg ccctattgat gttgaattga
    76261 cagagagttg tctgattgag aatgatgaac tggcactgtc tgttattcaa caatttagcc
    76321 aactaggtgc gcaagtgcat ctggatgatt ttggtaccgc ctactcttca ctttcgcaac
    76381 tggcgcgctt tccgatcgat gccatcaaac ttgaccaggt ttttgttcga gatattcaca
    76441 aacaacctgt ctcgcagtca ctggttcggg caatcgtcgc tgtggcccag gcattgaatc
    76501 ttcaggtgat cgccgaaggt gtagagagtg caaaggaaga tgctttttta accaagaatg
    76561 ggatcaatga gcggcaagga tttttgtttg ccaaaccgat gcccgccgtc gccttcgaac
    76621 gctggtataa acgctatctg aagcgcgcat aagtagatga atatatatac ttagcgctat
    76681 attccgtcat tatatatcgt attcccggat gataattagc cgttgctgtt aaacacttct
    76741 cttttagcgg caatttttgc attattcatt ttcccccttt tgctataagg atgtccttga
    76801 tgtctgaatt tgatgcccag cgcgttgccg aacgtattga tattgtgtta gatatccttg
    76861 tggcagacga ttaccactct gcgatccata atctcgagat acttaaagca gaactcttgc
    76921 gccaggttgc tgaatctacg ccagacatcc cgaaggcacc gtgggagatt taacaatcgt
    76981 gccttcaggt tttcctattt gaggatgccc ggcgcgtcag tattttttta tttagtatta
    77041 taacgttata agaattacag cgatgaattc ccgacaacaa actattctac agatggtcat
    77101 tgaccagggt caggttagcg taaccgatct ggcaaaagcc actggagttt ctgaagttac
    77161 cattcgccag gatctcaaca ccctcgaaaa actgagttac ctccgccgtg cacatggctt
    77221 tgcagtttcg cttgatagtg atgacgtcga aacccgtatg atgagcaact atacgctgaa
    77281 gcgtgaactc gccgagtttg ccgcgtcact ggttcaaccg ggcgaaacca tctttatcga
    77341 aaatggcagc agcaatgccc tgctcgcccg gactctgggg gagcagaaga aaaatgtcac
    77401 tatcatcacg gtcagcagct acatcgcgca tttgctgaaa gacgcgcctt gtgaagttat
    77461 tttgctcggt ggcgtgtacc agaaaaaaag cgaaagtatg gttggcccgt tgacacgcca
    77521 gtgcatccaa caggtacatt tcagcaaagc atttattggt attgatggct ggcaacctga
    77581 aactggattc accggtcgcg atatgatgcg taccgatgtg gtgaatgccg tgctggaaaa
    77641 agagtgcgaa gcgatagtcc tgactgacag ctcgaaattt ggtgctgtac attcatactc
    77701 catcggtccc gttgagcgat tcaatcgcgt gattaccgat tcgaaaatac gcgccagcga
    77761 tctgatgcat cttgagcaca gtaaactcac cgttcacgtc gttgacattt aaccatttcc
    77821 cttctctacg gatgatttgc agtttgacaa atcatccgct ctaagatgat tcctggttga
    77881 taattaagac tatttacctg ttattaacac tctcaagata taaaattatt atcagcgata
    77941 taacaggaag tcattatcac ctgcgtgata taaccctgcg cgcgagcgga tttcacggaa
    78001 taatttcacc aggcttattc ttagctatta tagttataga gagcttactt ccgtgaatca
    78061 taaattcagg agagagtatt atgtttgtaa cgagcaaaaa aatgaccgcg gctgttctgg
    78121 caattaccct ggcaatgtct ctgagtgcct gttctaactg gtctaaacgg gaccgcaaca
    78181 ccgcaatcgg cgcgggtgca ggggcattag gcggtgcagt actgaccgat ggcagtacgt
    78241 tgggtacatt aggtggcgca gctgtcggtg gtgttattgg tcaccaggtc ggtaaataat
    78301 cgtaaataaa tgtcgattat cgactcgtaa ttataatacg cttgattctg ttcagtacga
    78361 aataactgaa taatacacac agtaaaatat tcgaaagcca cggatatatc cgtggctttt
    78421 tatgttaacc gcctgcgagt tttaccttca tccctttcgc ttcaagtagt gattttaata
    78481 aatcacgctt atcgccctgg atttcaataa ctccatcttt aactgctccg ccgcagccgc
    78541 attttttctt cagttccgct gcaagttttg tcagttcggc atcatcgaga tcgataccgg
    78601 taatcaggca aacgccctta cctttacgtc cactggtctg acgctgaata cgcaccacac
    78661 catcgccttt agagcgtact ggggccgctt tgggctcatc aatacgcccg gtttctgttg
    78721 agtagaccag acggctgttg gaatcactca tcatgcactc cgctgtaaag aggcgttgat
    78781 cgctttcagc gtctgcgctg gatctaccga ttgcgttacc gggcgaccaa tcaccatata
    78841 atcaacacca gccgccaacg cctgttctgg cgtcataatg cggcgctggt caccagcttc
    78901 actcccctgc ggacgaatgc ccggcgtaac cagtttgaac tcctgaccga atacctgttt
    78961 aaagcgcaca gcttcctgag cagaacacac cacaccatca aggccacatt tttgcgtcag
    79021 tgctgccaga cgttctgcat aatctgcagg tgacagtgtc acgccaagat cggccaggtc
    79081 gctggcttcc atgctggtca acactgtcac agcaatcaaa agcggtgcat ctttgccaaa
    79141 cggaaccagt gcctcacgcg ctgcggtcat catacgcgcc ccaccagagg catgaacatt
    79201 caccatccac acgcctaagt cagctgcagc agcgacagcg tgcgctgcag tgttggggat
    79261 atcgtggaat ttcaggtcaa gaaagatatc aaaaccacgc tgttgaagtt cgcgcacaaa
    79321 ctgtggccca aacaatgtaa acatctcttt gccgaccttc agacgacaat cgcgtgggtc
    79381 gatcttgtcg acaaaggcca gcgcggcatc acgattatga taatcaaggg caacaaccac
    79441 aggagaattc gtaacagcgc gggaagaaga tgaagcagtt aacgtcatga ccagaccttc
    79501 ttgatgatgg gcaccggaag tggcgcgaaa caggtaaacg gcgagcattc tacccgtgga
    79561 aataaaaaat taacagttgc gatttctccc ctggcaggct ttttctgcgc cgccgttgcc
    79621 ggagcacaat gggacataaa taactagtat gttgtaacta aagtcggttt ttttaaaaaa
    79681 ttacaggcca tcaagaccgc gaatcggttt aatagttgac caggcacgac aagacggaca
    79741 atgccagtag agggtgtatg cggtaaaacc acatttctgg cagcgataac gaggcttact
    79801 acgtaccttc tcgccaacca tgtcacgcag caccatcagg ctctctttgg cacgcccttc
    79861 ttccgcttca tttaagtggt aatccattaa cttatggaac acacgcatgg tcggatgacg
    79921 ctgaagctgg cgcgtaatat agacctgtgc ggcctcacta ccgtcgcgcg cttcgatgat
    79981 atcagcaagc atcaattcag catcggcacc ggtgttctct tccaccgcgc gctgcaggaa
    80041 ttctgcccat tcggcagttt tacccaactg ctgatagcag gtttgcagca tttccagcgt
    80101 ttcgctgacc agttctctgt cctgggatat cacgcgttgc aaactttcga cggctttggc
    80161 gtattctcct ttcgccataa acacgcgtcc catcatgatg gatacgcggg cgctgttttt
    80221 atctgccgcc gccccttttt ttagcaaggt catggcacga tcgagatcgt cgctggccat
    80281 atgctgcagg gctaactcac agtagaaatg ggcaatttcg acgcgctgtt tatctttacc
    80341 cagcttcacc aggcgttcgg caacatcaat tgctttctgc cactcactgg tagcctggta
    80401 gatttgtagc aactgttgca gcgcgccaat gcggaagtca gtttcatcgg tcagctgatt
    80461 gaacatgtct tccgcgcggt catataaccc ggcggccatg taatcacgcc ccagttgttg
    80521 aatcgccaac agacgctgtt cataggtcag cgaggcgctt tccattaggg tctgatggat
    80581 gcgaatagcg cgatcaactt cgccacgcga acggaacagg tttccgagcg taaggtgggc
    80641 ttcaacggtg cctgtatcct ctttaagcat atcgagaaac aggtctaccg ctttatcctg
    80701 ttgattacta agcaggaagt taacccccgc tacgtaatca cgcgacaagc ggttggcttc
    80761 atcttgcttg ttttgttgcg cacttctgcg gcccatatac cagccatagg cagcggctac
    80821 aggcaaaagc agaaacaaca actccagcat agaaagttat tccttcgccg ctgacgagtg
    80881 ctgcactaca gccacgtcag tcgcgggtga aagctggttt tccagtcgct ttattttacg
    80941 ttcagcgcgc accagggaaa cacgaactcg cagccagaac aggccacaaa tcaaccaacc
    81001 gatagcaaaa cccgcagcaa acaataccgc cagcaatgtg gaaatacggt actccccttg
    81061 cgctaacaga taattaaacg tcacctgttg atcgttctgc gcacccaacg tgaccgaaat
    81121 cacgaagatc gctaacacca gtaagaaaat gagtaaatat ttcacattac ttcccgttat
    81181 gtggttagag cgaataaatt atggtcaact taccgcaata gccctttaaa gtaccatttt
    81241 cgcgccgtct gcgaaatggc atccacgcgc attgttgatg atttaagggc taaattcgga
    81301 aaatcaacca gcgttaactt tcttgttctc gttgcgctat ttcgcgattt tcttccgcag
    81361 gtggtgttaa tggcccgcaa attcgttgcg caagccacgt tgccaccgcc accagtgccc
    81421 acgaaatcaa cgtagctacc accagatcgc gtggccaatg catcccgagc agcaggcggc
    81481 ttcccatgac tcccgttgcc cagaccagca agatagcaat ggttaacgtt cgccgacgcg
    81541 gccacagcaa accaacggcc agcagtgccc aactggcagc aaacatcgtg tgaccggaag
    81601 gaaaggcaaa ccccgtctct ttctgccagt gtgaacgcaa atattgtgga atatttttct
    81661 cttcagccaa ctgttctttc actagatttc cgcgttctgc tcgctttaaa gtgtagaact
    81721 catcaaccgg aatatgatgt gttttttcca gccagataac aaaaggtcgt ggttcctgga
    81781 ctttgtcttt gatccaggat ttaacgcctt gtcccacaag gattgcggcc gccagaatgg
    81841 caaataatac aaaggcagcc ttaatgcgaa aacgcagaca ccagagaaac cagccgaata
    81901 aaatcaaatg tgtaatgacg ccccagggct gggtgacagt ttcagtaacc caaaaagccg
    81961 cttttagtag ccaactttgt tctccaggtt gccaacgcca gccagaaatc catacggcta
    82021 ctggcataac aagcaatagt gcagctccca ctgcggtacg tcttgcaatc gaacgcatgg
    82081 cctctccttt ttgataagtc ccacaatcat aactgaaaat gccagttcca ggaaaaattg
    82141 acagatttgt gccattccgt gaacgatcga cgcgtcgtga ttaggtgaac cccttctcgt
    82201 tatggcaaaa taagccaata cagaaccagc attatctgga gaatttcatg cagcttaaac
    82261 gtgtggcaga agccaaactg ccaaccccat ggggcgattt cctgatggtg ggatttgaag
    82321 aactggcaac cggacacgat catgtcgcgc tagtctatgg cgatatttcc gggcataccc
    82381 cggtacttgc gcgcgtccat tccgaatgtc tgaccggtga cgccctgttc agcttgcgct
    82441 gcgattgtgg cttccagctc gaagcggcat tgacgcaaat tgccgaggaa ggccgtggta
    82501 ttttgctgta tcaccgtcag gaaggtcgta acattggtct gctgaataaa atccgcgctt
    82561 acgcactgca ggatcaaggt tacgatactg tagaggctaa ccaccagtta ggcttcgccg
    82621 ctgatgagcg cgacttcact ctttgcgctg atatgttcaa actccttggc gtcaatgaag
    82681 tccgcttgtt aaccaataac ccgaaaaaag tcgaaattct gaccgaagcg gggattaata
    82741 ttgttgaacg cgtaccattg attgtaggtc gtaaccccaa taacgaacat tatctcgata
    82801 ccaaagccga gaaaatgggc catttgctga acaaataacc ctcttgcatt gtgtaattaa
    82861 tttgcttgcc ggaagcaaaa taaccggcaa gcaaatactt gttacttcaa catattacga
    82921 atgacataat gcaaaatgcc gtcgttctgg tagtaagtca actccgtcgc ggtgtcgata
    82981 cgacaacggc agggtacgac ttcctggcta ccatccgcgc gcgtaagcgt caccggaacc
    83041 gtcgcgccgg gttgtagatt ttgcagatcg ccaatatcaa tcttctcttc cccggttagc
    83101 cctaacgttt tacgcgttac gccttgcgga aattccagcg gcaggatgcc catgccaatt
    83161 aaattcgaac ggtgaattcg ttcaaacgat tcggcaatca ccacacgaat accaagcaga
    83221 cgcggacctt ttgccgccca gtcacggctg gagcctgatc catactcttt cccggcaatc
    83281 accgccagcg gcgtttgctc ctgcttatag cgcatcgcag catcataaat agagactacg
    83341 tcgctgtcag gtaaatgccg cgtcatcccc ccttcaacgc caggcaccat ttcattacgg
    83401 atgcgaatat tggcgaaggt gccgcgcatc atcacttcat ggttaccacg ccgcgaaccg
    83461 taggagttaa agtcttttcg ctcaacaccc cgaccttgta ggtatcgacc cgctgggctg
    83521 tcgggcttaa tactgcccgc cggagagata tggtcagtgg tgactgaatc ccccagcatt
    83581 gcgaggatcc gcgcaccgtg aatatcttcc acaggtgctg gcgttgcctg catttcatca
    83641 aagaaaggcg ataaacgaat ataggttgaa tcctcctgcc atccgtaggt atcggatcgt
    83701 gcgacgttaa tttctttcca ctctgcagtg ccttcaaaga cttctgcgta ctctttgcgg
    83761 aacatttctg tggagacttg ttctaccgca cgggcaattt cttgtgccga tggccagata
    83821 tctttcagat atattggatc gcctttacga tcatgaccaa taggctcaga agccaggttg
    83881 atattcatat ttcccgccag cgcataggca accaccagcg gcggcgaggc cagccagtta
    83941 gttttaacca gcggatggat acggccttca aagttacggt tgccggacag cactgcaccg
    84001 acggttaaat cgcctttttt gattgccgtt tcgataggat cgggcagcgg cccagagtta
    84061 ccaatacagg tggtacaacc gtatcccaca aggttaaacc ccagttcgtc gagatacggt
    84121 gtcagtttcg cttttgccag atagtcagaa acgactttcg aacccggtgc cagcgacgct
    84181 ttgacccacg gttgccgctt gaggccaaga gttacggctt ttttcgccag caagcctgcg
    84241 gccatcagca cacttgggtt agaagtgttg gtgcacgagg ttatcgcagc aatgaccaca
    84301 gcgccatcag gtaactgata ctgatgtccg ttcataacat aatcgaccgg ctggcgatct
    84361 ttatgcgtgg cattcacttc cagttcgtta ctggcggcaa atgcttttgg tacatcgggc
    84421 agtgcaacgc gatcctgtgg gcgtttaggc cctgccaggc tcgcttcaac gtcattcata
    84481 tccagatcta acgtactggt aaaaatcggt tcatcgcccg ggttacgcca catgccctgc
    84541 gctttggcat atttttcgac caactcgacc tgatcttcgc tgcgcccgct taaacgcatg
    84601 taatcgaggg ttacagcatc gattgggaag aagccacagg tggcaccata ttctggcgac
    84661 atattggcaa tggtggcgcg atccgccaac ggtagtgaat ccagaccatc accataaaat
    84721 tcgacgaatt tccccaccac gccatgtttg cgcagcattt gggtaacagt gagaaccagg
    84781 tctgtggcgg taataccttc acgtaatttt ccggtaagtt taaagcccac tacgtccggg
    84841 ataagcatgg aaaccggctg gcctaacatt gcggcttctg cttcgatgcc acccacgccc
    84901 caccccagca cgccaaggcc gttgatcata gtggtgtgcg agtcagtacc aacgagtgta
    84961 tccggataag caatccattc accgtcctgc aattcgctcc acactgcttt gccgagatat
    85021 tcgaggttaa cctgatggca aatgcctgtg cctggcggca cgacgctaaa ccgactgaac
    85081 gcttgctttc cccatttcag gaacacataa cgttcgtggt tgcgctccat ttccaggcgt
    85141 acgttttctt caaatgcctc atcatcacca aaacgatcga cggtcaccga gtggtcaatg
    85201 accaggtcga ccggtgagag cgggttaacc tttgcagtat cgccaccgag gcgtttaacc
    85261 gcttcgcgca ttgccgccag atcaacaacg gcaggtacgc cggtaaagtc ctgcatcagc
    85321 acccttgccg ggcggtaggc aatttcacgg tcagcatggg catttttcag ccatcccgcc
    85381 agcgcgtgga tatcctcttc ggtaaccgag ttaccatcct gccagcgcag cagattttcg
    85441 agcaaaactt tgagtgactt gggtagacgg gtgatatcgc ccagtgattt ggcagcaagc
    85501 ggcaggctgt agtagtggta agttttatct ttggcctgca acgcgtcctt actggcttct
    85561 cgtagggttg acgacatagc tcctccttaa tgacagggtt gcgaagccct gattctcttc
    85621 agggtgaata ttaaagataa cacaaacatc gcgtaacgat ttgataacaa cccaaattga
    85681 taaaagagga gaaaccttgt ggcaaagcaa atgacaaccc cgccgcagcg gggtcaagga
    85741 tcagtgagta atgatccaga ggagttcggc ccagaagagc atcgacagag aaaatgcacc
    85801 catccaggac caatatttca tatttgtgtt gatcataggc tacaccctta acggttatta
    85861 ctttgtgttg agataactca acctgttatt gctgcggtgt attaaaagcc ctttaattca
    85921 ttaaaaatgg gtaataataa tgatgataat tattgtttcg gctgatcatc ttcagcaaac
    85981 agttctataa aggcttgctg ctgcgatgtt agttgccagt cgtctggaat aacgacggaa
    86041 cattcccgtt tcgtttcggg ttcattgtca ctatgctggc ataatggtga cgactccagt
    86101 tgctcctgac caaccatagt tatccccaca ctttccaaat cagcagtgcg acaaccaccc
    86161 agaaaagtgc agaaccaaga aaaaccgcca gccaggcttt acgttttaat tcaggatcgc
    86221 ggcggggttc ctgatttcca gaaggcattg ctaacctcat ataatcgcca tcacttatca
    86281 gcaagacgtt gaacgattgg tgtaattaat catacgatga gacaaatcct aaagaaactt
    86341 tagtcaaaaa tccagcgaat ttacgaatcg tctccagtga ataattcaat cttttgacct
    86401 gaaatagata tttgaaaact ggaatttgcg aggcgggtaa ttagacacta ctttacaggt
    86461 aatttaccct ataaatatga gggataaaaa tattgtgcac ggtatacaaa taagtaacaa
    86521 aagagaatat attccggcac cttcgctaca taaaaggtgc cgaaaataac gcaagaaatt
    86581 atttttccgg cagttttata tctttaaaca tgacctcaat ttcttcattg gagcgcaatg
    86641 cgacagccgc atcaacgaca tcacgcgtta aatgcggtgc aaaacgctga atgaaatcat
    86701 acatataact gcgcaaaaaa gtactacggc gaaaaccaat tttggttgta ctgtggctga
    86761 agatatcgtg agcatcaaca cgcacaaggt ccggatcggc gaccggatcc accgccatgc
    86821 tggcaatgac ccctaccccc agccctaacc ggacgtaagt tttaatgacg tcagcatccg
    86881 ttgccgtgaa aacgatacgc ggcgttaacc ctgcgcgatt aaaggcagta tccagttctg
    86941 aacgtccagt aaagccgaag gtatatgtca ccaacggata ttgcgccagt tcttcaatgg
    87001 taatggcttt tttgcctgcc agcgggtgat ccggagtgac tacaatagcc cgattccagt
    87061 ggtagcacgg taacatcact aaatcttcat acagatgcag cgcttctgtg gcgatagcga
    87121 aatctgcatt acctttagag acggcatcag caatttgtgt cggcgaaccc tggtgcatat
    87181 gcaaagaaac gcggggataa cgctcaataa agcctttgat gacgtttggt aacgcgtagc
    87241 gtgcctgggt atgcgtggtg gcgatataca gcgagccttt atccggccag gtgtgctcac
    87301 cggcaaccga ttttatggca tcaactttcg acaggacttc gcgagcgata cgaattattt
    87361 cttgtcctgc tggcgttacc tgcgtcaggt gcttgccgct tcgggaaaaa atttgaatgc
    87421 ctagctcgtc ttccagcatt ctgacttgtt tactgatccc gggttgtgat gtgtaaagtc
    87481 cttccgctgt tgatgagaca ttcaggttat gattgaccac ctcaacaata tagcgaagtt
    87541 gttgtaattt catgttaaac catccactta gacgccatca gaacatcgtt ttttcgtcac
    87601 acctggcggg tgactaatac gatttaataa taaaaaaggt gctaactata accactatat
    87661 catttatagc cttagtgtat aggtgtgaag aaaaaataat atgcaataaa aaagggccgc
    87721 tttcgcgacc ctttgtttat aaaaacctga cagaattaaa ggttattttt tcccttcaac
    87781 ccatttgcca tcaacataaa atgctgacca gccagtcgcc tttccgtctt tttccgaaga
    87841 gacatattgc tgtttggttt tacggctaaa gcgaaccata gtcttattac cttccggatc
    87901 ctgctgcggc gcatcggcca gataacgcag tttttccggc agacgatcgc ggaagcgata
    87961 cagctcttcc accagcggcg cacgcgtttc acgcgatttc gggaaggtat tggccgccag
    88021 gaacacaccg gcagcaccgt cacgcagcac gaaataggca tctgattttt cgcacggcag
    88081 ctccggtaat ggtaccggat cttctttcgg tggagccact tcgccgttac gtaaaatctt
    88141 acgcgtgttt ttacactctt cgttggtgca ggccatatat ttaccgaaac gccccatttt
    88201 caggtgcatt tcagaaccac atttttcaca ctcaacgatc gggccgtcat aacctttaat
    88261 gcggaattcg ccctcttcga tctcgtaacc gtcgcaggtt gggttattac cacagacatg
    88321 caacttacgt ttcggatcga tgagatagct gtccatcgcc gtgccgcatt tggggcaacg
    88381 acgttttgcg cgcagcgcgt tggtttcagc gtcttcgcct tccagcacgt tcagcacttc
    88441 gttttccggc accaggttaa tggttgtttt gcaacgctct ttcggcggca gcgcatagcc
    88501 agaacagcca aggaataccc cggtgctcgc tgtgcgaatc cccattttgc gaccacaggt
    88561 cgggcagtcg atgctggtca gaaccatctg gttcggacgc atacccccct cttccggatc
    88621 tttttcagct ttatctaact gctgagtgaa atccgagaag aagtgatcca gtacagcttt
    88681 ccactctgct tcgtgatttg ccacctggtc aaggctgttt tccatctgcg cggtgaagtc
    88741 gtagttcatt aactcgcgga aattctcttc caggcgatcg gtgacgattt cgcccatttt
    88801 ttccgcatag aaacgacgat tttctactcg cacatagcca cgatcctgaa tggtcgaaat
    88861 gatcgacgca taggtagacg gacgaccgat accacgtttt tccagttctt taaccagcga
    88921 tgcttcactg aaacgggctg gcggcttggt aaagtgctgg gctggtgtca gttcaacgag
    88981 cgtcagagca tcgcctttat cgactgcagg taagatacga tcttcatcgc ctttacgcag
    89041 tgcaggcatc actttcgtcc agccatcaaa gcgcaaaata cgaccgcgtg ctttcaggcg
    89101 gaaatcgccc gcaccaacgg tcagcgtcgt ggagtcatat ttcgctgggg tcatctggca
    89161 ggcaacgaac tgacgccaga ttaactggta cagtttctgc gcatctgctt ccatatcctt
    89221 cagcgattcc gccatcacat tgacgtcaga aggacgaatc gcttcgtgcg cttcctgtga
    89281 gttttctttg ctggcgtact gattcggact ttccggcaga tatttcttac caaaattatc
    89341 gctgatataa ccgcgaacca tatttaccgc gtcctgactc aggttagtgg agtcggtacg
    89401 catgtaagtg atatagcctg cttcatacaa acgctgcgcc atcatcatgg tttttttcac
    89461 gccaaatcca agacgggtgc tggcagcttg ttgcagcgta gaggtaataa aaggagcgcc
    89521 aggtttactg gttgtcggtt tgtcttcacg ttccagcacg ctgtagcgcg ctttttccag
    89581 cagacttacc gcagcctgag tttgttcttt gttgaccgga cggaacggtt tgtcgttctg
    89641 atgagtcacc tgcaacgcca acgcttcacc agatggcgtg gtcgtgctgg catcgacttc
    89701 ccagaactct tccggcacga acgctttaat ttcacgctca cgctcgacca ccaggcggac
    89761 tgccaccgac tgcacacgac cggcagacag accacgagcg atctttttcc atagcagcgg
    89821 cgaaaccata taccccacca cgcggtccat aaagcgacgc gcctgctggg cattaacacg
    89881 atcaatattc agctcacccg gtttgttaaa tgcctggcgg atcgcgtttt tagtaatttc
    89941 gttaaacacc actcggctat agcgcgcatc atcaccacca atcacttccc gcaggtgcca
    90001 tgcaatggct tccccttcgc ggtcaaggtc ggttgcgaga tagatgtggt cggctttttc
    90061 agccagttgt ttcagttcag agacgacctt ctctttacca ggcaacactt catagtgcgc
    90121 ctcccaattg tgccacgggt caacccccat acggttgacg agagcgccac gttcatcctt
    90181 tttaggcttt ttagccgtct tggtggaggt agagtcggca ctctttttgg cagctgagcc
    90241 actggtcggc aaatcgcgga tgtgaccgac gctggatttc accacgtagt cactacccag
    90301 atacttgttg atcgttttgg cttttgccgg ggactcaacg atgacaagag ctttacccat
    90361 attcacctta cctaatttga ttcttccagg aatgcgtcgc acgttgataa accttccact
    90421 ggcaacgagt tactgatatg gactcgctct ctgcggatat caaccccttg tcgtgactct
    90481 gttcacgaaa attcaggtga ctgagttaac aggtcttggc ctacaggcgc tatagcacga
    90541 cgaaattttg gtcgaatgtc aagcaaatct attgccagat tgacgaaagc gtcacactgt
    90601 acctgataaa atccgatacg caactttatt agcatgcaaa atccaaagtt gccagtaagg
    90661 cgctcttgcc accagcgtgg tatatttgtc tgcaagccat gcctggcgta gactaatgtc
    90721 cttgtttaag gagtaaataa tgaacaaaga gacacaaccc atcgaccgcg aaacgctgtt
    90781 gaaagaggcc aacaaaatca ttcgtgagca tgaggatacg ctcgcaggga ttgaagccac
    90841 cggcgtcaca cagcgtaatg gcgtgctggt atttaccggc gactactttt tagatgaaca
    90901 aggattgcca acagccaaaa gcaccgcggt gtttaatatg ttcaaacacc tggcccatgt
    90961 gctttcagaa aaatatcatc tggttgatta aagcaaaacg cgaggtctta ccctcgcgtt
    91021 tgtcttttac atcaatggct tttgcccacg ctgccaccag cgtagcaata atcgatcggc
    91081 gctctctgcc gcgctgccgg tgaatcggtc aatgagtcgt ttacgctgca tatagcgtac
    91141 attgaccact tctcggcctt ccatcaggct aagaataact tcatcactgg tgttgatttc
    91201 atcaaccagg cccttctcta ccgcctgttg tccgtaccag tgttcacccg ttgccacctg
    91261 ttcaatatcc agagacggac gcatacgctt cacaaaatct ttaaataact gatgcgtttc
    91321 gttcagctct tcgcggaatt tctcccgccc ttcttcggta ttttcaccca gcaaggtcag
    91381 cgtacgctta tactgcccgg cggtgtgcag ttcgatatca atatctttgc ttttcaggaa
    91441 gcggttaaag ttgggcattt gcgccaccac cccaatggaa cccacaatag caaacggtgc
    91501 ggaaacaatt ttgtccgcca cacaggccat catgtaacca ccgctggcag caactttgtc
    91561 taccgtaaca gttaatggaa tgtttttatc acgcagacgc tgcagctgcg aagccgccaa
    91621 cccgtaacca tgcaccatgc caccagggct ttccagacgg agcacaacct gatcctgcgg
    91681 tttgaatgct gcaagtaccg ccgttatctc ttcacgtagc gagttcactt catgggcgtc
    91741 catgctgcct ttaaagtcca gcacccagac gcggggttta ctgtcagttg acacctcgcc
    91801 cagtttggct ttcgcttttg ctgctttcgc ttcttgcttg tgctttttct tctgcgcttt
    91861 gtgccactgt ttttgctgat gtgagtccat cagcgccgcg gccagttctt ctttcatctc
    91921 cttatactgt tcgctgagat tgttgacccg taactcgcca cgctggcgtt tattacgttg
    91981 agcaacattg acaataatgg cggcaatcgc cgcaatcgct agcacaacgg taacgatttt
    92041 cgccaaaaac aaaccatatt cagacaacaa ttccacgagt ccccaccttg gttaaacaac
    92101 gctttggctc acagtttaca acaggcatgt taagccgtct cgcagagatg ttattgcagc
    92161 gaaagtatga caaagtatcc attttgcggt gttatcgccc gaggctgatt gaaatgccct
    92221 tctgtttcag gcataaagcc ccaaagtcat aaagtacact ggcagcgcga cagacaacgt
    92281 cgttgaggag tagccatgca ttaccagcca aaacaagatt tactcaatga tcgcattatc
    92341 ctggtgacgg gagccagtga tggtattggt cgtgaagccg cgatgacgta tgcacgctat
    92401 ggtgcgacag tgattctgtt gggccgtaat gaagaaaaat tacgtcaggt agccagccac
    92461 ataaacgaag aaactgggcg tcagccacag tggtttattc tcgatttgct gacctgcagg
    92521 tccgaagatt gccaacaact ggcacagcgc attgccgtta attatccgcg tctggatggt
    92581 gttttgcata atgccggatt actcggcgat gtttgtccaa tgagcgaaca aaatccacag
    92641 gtctggcagg acgtcataca ggtcaacgtt aatgccacct ttatgctcac ccaggcacta
    92701 cttcctttat tactcaaatc ggacgccggt tcactggtct ttacttcatc aagcgttgga
    92761 cgtcagggac gagccaactg gggtgcatat gcagcgtcga aatttgccac cgaagggatg
    92821 atgcaggtac tggccgatga atatcagcag cgcctgcgtg tcaactgcat taacccaggc
    92881 ggtacgcgca ccgcaatgcg tgccagcgcc ttcccgaccg aagatccaca gaaacttaaa
    92941 acacccgctg atatcatgcc gctctacctc tggctgatgg gcgatgacag ccgccgtaaa
    93001 accggcatga cctttgacgc ccaaccgggc cgtaaaccag gaatttccca atgagtgatg
    93061 aacgctacca acagcgtcag cagcgagtga aagaaaaagt agatgctcgt atggcccagg
    93121 cccaggatga acgcggtatt atcatcgtct ttaccggcaa tggaaaaggc aaaaccaccg
    93181 cggcatttgg tacggcaaca cgcgcagttg gtcacggaaa aaaagtgggc gtcgtgcagt
    93241 ttattaaagg cacctggcct aatggcgaac gcaatctgct ggagccacat ggcgttgagt
    93301 ttcaggtgat ggcaacgggc tttacctggg atacacaaaa ccgcgagtct gataccgccg
    93361 cctgccgcga agtctggcaa catgcaaagc ggatgcttgc tgattcctca ctggatttgg
    93421 ttttgcttga tgagctgact tatatggtgg cgtatgacta tttgccactg gaagaagtgt
    93481 tgcaggcgtt aaatgaacgt ccacatcagc agacggtgat tatcacgggt cggggctgtc
    93541 atcgggatat actcgaactg gcggatacgg taagtgagtt acgccccgtg aaacatgcgt
    93601 ttgatgctgg cgtaaaagcg cagataggga ttgattacta atctgtaaaa atgccggata
    93661 tgacgtccgg catacgttga ttaaccgtta ttacgcccac cagaacgacg tccgccgctc
    93721 acctgactgt gacgtttcac cgcacgacga atctgattcg ccttcatgcg acgacggtct
    93781 ttttctacag cgactttaga gctggtttct ggcggcaact ccaccagttc gcgcagatag
    93841 ttagtctggg caagatccag ctccgtccag ccgccgcgtg gcagaccttt cggcagtggg
    93901 atatcaccgt aacgaacacg gatcaggcgg cttacctgca cgccaaccgc ttcccacaga
    93961 cgacgaactt cacggttacg gccttcggtc agagtcacgt tgtaccactg gttgatccct
    94021 tcgccgccgc tgaacttgat ggttttaaag gctgccggac catcttccag ctgcacacca
    94081 cggcttaaat cacgcagttt cgcgtcgtca acctgaccaa atacacgcac ggcatattca
    94141 cgttcaactt cacggcttgg atgcattaaa cggttagcca gttcaccgtc ggtggtgaac
    94201 agcaacagac cacaggtgtt aacgtccaga cgacctacgg caatccagcg tgcaccgcgc
    94261 agctttggca gacggtcaaa caccgttgga cgcccttccg gatcgttacg ggtacacaac
    94321 tcaccttccg gtttataata ggccagaacg cgacaaattt gttcagcgga ctcacgtacc
    94381 gaaatcaggt gaccatcgat acggattttc aatccagggg taacttcaac gcgatcgccg
    94441 agtttagcaa ttttgccatc aacactcaca cgaccggctt caataataga ttcgatttca
    94501 cggcgagaac cgtggccggc acgcgccagc actttctgta gcttttcgct catagagctt
    94561 ccttttggtg tcgccttccc aggcgtctga tatgcttttg tgcaacagtg agtcagaact
    94621 tacatctgac tcactgatag taaagtctgt ttgaactctt caggccttaa gtaaacgact
    94681 cgacgtttta actggcctgg ctttgaggcg caacatacta cactatcttt atggttaaag
    94741 cttgtttcga ttaacatcag cttaattacc agccaccacc gcctccgcca cccgcagcac
    94801 caccggaata acttcgcccc aataaactgg tattggcccc cagactcagg tgcatctgta
    94861 atgagaacaa aacgccacta cgggcaattt ccggggcgcg aacagccgct gtgagtgatg
    94921 gggtgatacg tgctacccat tctcttccaa ggtttgctga gacagcccaa cctagcaacg
    94981 cgtaatccag tttccgtgtt tcccctttcc gccgacgccc actgtgtgtt gctgcctctt
    95041 tacgcgcgag atagttaacg atagcttcac cgtgggcata acgttgctgc ccagtttggg
    95101 taaactttgg cagaaaaata taccccatgc cagtgagata accggtcagg aaaacagcgc
    95161 taaaataacc cgctggcata taccagtaaa agaatacatc tccgacattg atatagagag
    95221 aataaaaggc cacgcctgaa caaatcagcg gtaaaaacaa agtgatgatt acacgattaa
    95281 cgcaaggctt gtcatcatcg cgggcaaaca aaagctcaag ggaacaaaat aaaaaaggaa
    95341 gaagccagca gatcataata aacatatcgc ccaccagact cattcctgcc cagcctacag
    95401 gactcaggtt accgtaaaga atgaccgcca atattgccag tccaccccat tgcaaaaaag
    95461 tatcaggccg gtaaaacgaa gactttttac gcggcagata aaatttctgc atccgtaaaa
    95521 catctaccga cgtaaattgc ggcctgcgct tccagagata taaccatcca caaaccagga
    95581 atagacacgg aaaccaaatt aacaacgatg acgtggaggg taaaagaaga tggctgaaaa
    95641 tattcgtcgt ttgtggagcc ggggcattgc taagtaacga gtgaggccaa cggtagagta
    95701 ccgtgaaatc ttcacgataa aacggatcgc tggattcaat tcttccgtcc tcaaggatgc
    95761 ggccatttcg atatgtgtcg ccctcttctc cagtaaagag atcgatttcg ctaaatggat
    95821 tacccgcgat atcagggaac ttgagtgaaa atcgggtctt atagatttca aacggccagt
    95881 ggttaccagt cacgttccag attaacagat ctgaatctcc ctcacgcagg aaagcatttt
    95941 taacctgata atgaatttga taatgatagt cgccttgagg caagaaacgt tgtttatcgc
    96001 ccacgacaat actcattaac ccgatatgcc agtcaagatg ccagggttca ggaataccat
    96061 cacgggtgac gccaacaata tgatagttca gcagtgcagc gtccccatca ggctgcatcc
    96121 atagtcgtgg taaatcacga taaaatccgt gcttaatctg ctgatttcgc gcctgaaaat
    96181 gaatattttc tttgatgtcg agcaccccat ccggttgcag agtggcttca acatcaaaga
    96241 gcgagatccc ttcttcataa gaaggcatct ccgtgttttt cgcataactt aacgaagaga
    96301 aaaaaagccc aactatcaac agcaaaatgc agcgaaactt ccctgccata gtcttccatc
    96361 cgtggaacgc aatttgttat aagaaaggct tcacatcacc tacgccttca cgcaccacga
    96421 ccggcgtatc atcggtaaga tcaataaccg ttgttggttt ctgcccgaga tagccgccgt
    96481 gaataatcaa atcgacctgt ttttccagac ggtctttgat ttcttcggga tcggattcgg
    96541 taaattcgct gcctggcagc attagcgaag tggaaagcat cggttcgccc agtgcttcaa
    96601 gcaacgcctg cgcaatcggg ttagaaggca cacgcatacc gatggttttg cgtttttcct
    96661 gcaacagacg gcgtggcacc tccttcgtcc ccttcaggat gaaggtatag ttgcccggcg
    96721 tgttgttttt cattaaacga aacgcaacgt tatcgacaaa tgaataggtc gagagttcag
    96781 aaagatcgcg acacatcagg gtaaagttgt gaccgtccgg cagctggcga atacgacaaa
    96841 tacgctccat ggcgttttta tcttcaattt tacagccgag cgcatagccg gaatcagttg
    96901 gataaacaat caccccgcct ttacgcacga tctccaccgc ctggttgatt agacgttgct
    96961 gcgggttatc aggatgaata taaaaaaact ggctcataat tccctctctg tggtgttctg
    97021 cggctgttcc catagctgcc agacgccttc aacgcctgcg ggcagccaga gtttacgacc
    97081 cagttcgatc cacgggcatg gctgatgaaa atcagatcct tgtgatgccc ataaatggtg
    97141 ctggcgggca agcgtcgcca gctgggtacg ttcattgggc gattgctggc actgcgcgac
    97201 ttccatcgcg tcgccgtcgt gttcggcaaa atgcgcaacc aatcttttca gccatttagc
    97261 actaagattg taccgcccag gatgagctag caccgcctta ccgccagaat gatgaatgac
    97321 atcaatagct tgttctattg tacaccactg tggcggaacg tatccggttt tcccgcgcgc
    97381 cagatacttt ttaaagacat ccgccattga actggctttg ccgcactcaa caaggaaacg
    97441 cgcaaaatga ccgcgcgtca ctgcgccgcc ctgcgccagt cgttgtgcgc cttccagcgc
    97501 cccaggtata tgcgcttttt caagtctttc ggcaatttgc tgcgcccgct gattgcgccg
    97561 ttctgtctgc tgcgcgagga actcacacat tagcggatga gtaatatcaa tattcagccc
    97621 gacaatatga atttcatgat tttcccagac cgtggaaatt tccacgccgg gaataagatt
    97681 cagcgccagt cccgaacgtg aaatttcctc tcttgctggc gcgattgcag ctgtggtgtc
    97741 atggtcggtg atcgccaggg tgccgacgcg catctcgact gcacggtgca ccaatgcttc
    97801 tggcgtcagg cagccatcgg aagctgtggt atggctgtgc aggtcgtaaa tcactgcgta
    97861 attcgtgtcg ctcaaggcgc actcccgttc tggataatgt tttttgcgcc gacatcataa
    97921 cggttctggc aaatattctg aaatgagctg ttgacaatca atcatcgaac tagttaacta
    97981 gtacgcaagt tcacgtaaaa agggtatcga caatgaaagc aattttcgta ctgaaaggtt
    98041 ggtggcgcac ttcctgaaac gggcagtgta ttcaccatgc gtaaagcaat cagataccca
    98101 gcccgcctaa tgagcgggct tttttttgaa caaaattaga gaataacaat gcaaacacaa
    98161 aaaccgactc tcgaactgct aacctgcgaa ggcgcttatc gcgacaatcc caccgcgctt
    98221 tttcaccaat tgtgtgggga tcgtccggca acgctgctgc tggaatccgc agatatcgac
    98281 agcaaagatg atttaaaaag cctgctgctg gtagacagtg cgctgcgcat tacagcttta
    98341 ggtgacactg tcacaatcca gtccctttcc ggcaacggcg aagccctgct gacactactg
    98401 gataacgccc tgcctgcggg tgtggaaaat gaacaattac caaactgccg tgtgctgcgc
    98461 ttcccccctg ttagtccact gctggatgaa gacgctcgct tatgctccct ttcggttttt
    98521 gacgctttcc gcttattgca gaatctgttg aatgtaccga aggaagaacg agaagccatg
    98581 ttcttcggcg gcctgttctc ttatgacctt gtggcggggt ttgaagattt accgcaactg
    98641 tcagcggaaa ataactgccc tgatttctgt ttttatctcg ctgaaactct gatggtgatt
    98701 gaccatcaga aaaaaagcac ccgcattcag gccagcctgt ttgctccgaa tgaagaagaa
    98761 aaacaacgtc tcactgctcg cctgaacgat cttcgccagc aactgaccga aaccgcgcca
    98821 ccgctgccgg tggtttccgt gccgcatatg cgttgtgaat gtaaccagag cgatgaagag
    98881 ttcggtggcg tagtgcgttt gttgcaaaaa gcgattcgcg ccggagaaat tttccaggtg
    98941 gtgccatctc gccgtttctc tctgccctgc ccgtcaccgc tggcggccta ttacgtgctg
    99001 aaaaagagta atcccagccc gtacatgttt tttatgcagg ataatgattt caccctgttt
    99061 ggcgcgtcgc cggaaagttc gctcaagtat gacgccacca gccgccagat tgagatctac
    99121 ccgattgccg gaacacgtcc acgcggtcgt cgcgccgatg gctcgctcga cagggacctt
    99181 gaaagccgta ttgaactgga aatgcgtacc gatcataaag agctttctga acatctgatg
    99241 ttggtggatc tcgcccgtaa tgacctggca cgcatttgca cccccggcag ccgctacgtc
    99301 gccgacctca ccaaagttga ccgttactct tacgtgatgc acctggtctc ccgcgtggtc
    99361 ggtgagctgc gccacgatct cgacgccctg cacgcttacc gcgcctgtat gaatatgggg
    99421 acgttaagcg gtgcgccgaa agtacgcgct atgcagttaa ttgccgaggc tgaaggtcgt
    99481 cgccgcggca gctacggcgg cgcggtaggt tatttcaccg cgcacggcga tctcgacacc
    99541 tgcattgtga tccgctcggc gctggtggaa aacggtatcg ctaccgtgca agccggtgct
    99601 ggcgtagtcc ttgattctgt tccgcagtcg gaagccgacg aaacccgtaa taaagcccgc
    99661 gctgtactgc gcgctattgc caccgcgcat catgcacagg agacgttcta atggctgaca
    99721 ttctgctgct cgataatatc gactctttta cttacaacct ggcagatcag ttgcgcagca
    99781 atggtcataa cgtggtgatt taccgcaacc atattccggc gcagacctta attgaacgcc
    99841 ttgcgacgat gagcaatccg gtgctgatgc tttctcctgg acccggtgtg ccgagcgaag
    99901 ctggttgtat gcctgaactg cttacccgcc tgcgcggtaa gctgcctatt attggcattt
    99961 gccttggtca tcaggcgatt gtcgaagctt acgggggcta tgtcggtcag gcgggcgaaa
   100021 ttcttcacgg taaagcgtcg agcattgaac atgacggtca ggcgatgttt gccggattaa
   100081 caaacccgct gccggtggcg cgttatcact cgctggttgg cagtaatatt ccggccggtt
   100141 taaccatcaa cgcccatttt aatggcatgg tgatggcggt gcgtcacgat gcagatcgcg
   100201 tttgcggatt ccagttccac ccggaatcca ttctcaccac ccagggcgct cgcctgctgg
   100261 aacaaacgct ggcctgggcg cagcagaaac tagagccaac caacacgctg caaccgattc
   100321 tggaaaaact gtatcaggcg cagaccctta gccagcagga aagtcatcag ctattttcag
   100381 cggtggtacg tggtgaactg aaaccggaac acctggcggc ggcgctggtg agcatgaaaa
   100441 ttcgcggcga gcacccgaac gagatcgccg gagcagcaac cgcgctactg gaaaacgccg
   100501 cgccgttccc gcgtccggat tatctgtttg ccgatatcgt cggcaccggc ggtgacggca
   100561 gcaacagcat caatatttcc accgccagtg cgtttgtcgc cgcggcctgt gggctgaaag
   100621 tggcgaaaca cggcaaccgt agcgtctcca gtaaatctgg ctcgtcggat ctgctggcgg
   100681 cgttcggtat taatcttgat atgaacgccg ataaatcgcg ccaggcgctg gatgagttag
   100741 gtgtatgttt cctctttgca ccgaaatatc acaccggatt tcgccatgca atgccggttc
   100801 gccagcaact aaaaacccgc accctgttca atgtgctggg gccattgatt aacccggcgc
   100861 atccgccgct ggcgttaatt ggtgtttata gtccggaact ggtgctgccg attgccgaaa
   100921 ccttacgcgt gctggggtat caacgcgcgg cggtggtgca cagcggcggg atggatgaag
   100981 tttcattaca cgcgccgaca atcgttgccg agctgcatga cggcgaaatt aagagctatc
   101041 aattgaccgc tgaagatttt ggcctgactc cctaccacca ggagcaactg gcaggcggaa
   101101 caccggaaga aaaccgtgac attttaacac gcttgttaca aggtaaaggc gacgccgccc
   101161 atgaagcagc cgtcgcggcg aatgtcgcca tgttaatgcg cctgcatggc catgaagatc
   101221 tgcaagccaa tgcgcaaacc gttcttgagg tactgcgcag tggttccgct tacgacagag
   101281 tcaccgcact ggcggcacga gggtaaatga tgcaaaccgt tttagcgaaa atcgtcgcag
   101341 acaaggcgat ttgggtagaa gcccgcaaac agcagcaacc gctggccagt tttcagaatg
   101401 aggttcagcc gagcacgcga catttttatg atgcgctaca gggtgcgcgc acggcgttta
   101461 ttctggagtg caagaaagcg tcgccgtcaa aaggcgtgat ctgtgatgat ttcgatccag
   101521 cacgcattgc cgccatttat aaacattacg cttcggcaat ttcggtgctg actgatgaga
   101581 aatattttca ggggagcttt gatttcctcc ccatcgtcag ccaaatcgcc ccgcagccga
   101641 ttttatgtaa agacttcatt atcgaccctt accagatcta tctggcgcgc tattaccagg
   101701 ccgatgcctg cttattaatg ctttcagtac tggatgacga acaatatcgc cagcttgccg
   101761 ccgtcgccca cagtctgaag atgggtgtgc tgaccgaagt cagtaatgaa gaggaactgg
   101821 agcgcgccat tgcattgggg gcaaaggtcg ttggcatcaa caaccgcgat ctgcgtgatt
   101881 tgtcgattga tctcaaccgt acccgcgagc ttgcgccgaa actggggcac aacgtgacgg
   101941 taatcagcga atcaggcatc aatacttacg ctcaggtgcg cgagttaagc cacttcgctg
   102001 acggttttct aattggttcg gcgttgatgg cccatgacga tttgcacgcc gccgtgcgtc
   102061 gggtgttgct gggtgagaat aaagtatgtg gcctgacgcg tgggcaagat gctaaagcag
   102121 cgtacgacgc gggcgcgatt tacggtgggt tgatttttgt tgcgacatca ccgcgttgcg
   102181 tcaacgttga acaggcgcag gaagtgatgg ctgcggcacc gttgcagtat gttggcgtgt
   102241 tccgcaatca cgatattgcc gatgtgctga acaaagctaa ggtgttatca ctggtggcag
   102301 tgcaactgca tggtaatgaa gatcagctgt atatcgatac gctgcgtgaa gctctgccag
   102361 cacacgttgc catctggaaa gcattaagcg tcggtgaaac cctgcccgcc cgcgagcttc
   102421 agcacgttga taaatatgtt ttagacaacg gccagggtgg aagcgggcaa cgtttcgact
   102481 ggtcactatt aaatggtcaa tcgcttggca acgttctgct ggcggggggc ttaggcgcag
   102541 ataactgcgt ggaagcggca caaaccggct gcgccggact tgattttaat tctgctgtag
   102601 agtcgcaacc gggcatcaaa gacgcacgtc ttttggcctc ggttttccag acgctgcgcg
   102661 catattaagg aaaggaacaa tgacaacatt acttaacccc tattttggtg agtttggcgg
   102721 catgtacgtg ccacaaatcc tgatgcctgc tctgcgccag ctggaagaag cttttgtcag
   102781 cgcgcaaaaa gatcctgaat ttcaggctca gttcaacgac ctgctgaaaa actatgccgg
   102841 gcgtccaacc gcgctgacca aatgccagaa cattacggct ggcacgaaca ccacgctgta
   102901 tctgaagcgc gaagatttgc tgcacggcgg cgcgcataaa actaaccagg tgctcggtca
   102961 ggctttactg gcgaagcgga tgggtaaaac cgaaatcatc gccgaaaccg gggccggtca
   103021 gcatggcgtg gcgtcggccc ttgccagcgc cctgctcggc ctgaaatgcc gtatttatat
   103081 gggggccaaa gacgttgaac gccagtcgcc taacgttttt cgtatgcgtt taatgggtgc
   103141 ggaagtgatc ccggtgcata gcggttccgc gacgctgaaa gatgcctgta acgaggcgct
   103201 gcgcgactgg tccggcagtt atgaaaccgc gcactatatg ctgggcaccg cagctggccc
   103261 acatccttat ccgaccattg tccgtgagtt tcagcggatg attggcgaag aaaccaaagc
   103321 acagattctg gaaagagaag gtcgcctgcc ggatgccgtt atcgcctgtg ttggcggcgg
   103381 ttcgaatgcc atcggcatgt ttgcagattt catcaacgaa accaacgtcg gcctgattgg
   103441 tgtggagcct ggtggtcacg gtatcgaaac tggcgagcac ggcgcaccgc taaaacatgg
   103501 tcgcgtgggt atctatttcg gtatgaaagc gccgatgatg caaaccgaag acgggcagat
   103561 tgaagaatct tactccatct ccgccggact cgattttccg tccgtcggcc cacaacacgc
   103621 gtatcttaac agcactggac gcgctgatta tgtgtcaatt accgatgatg aagcactgga
   103681 agccttcaaa acgctgtgcc tgcacgaagg gattatcccg gcgctggaat cctcccacgc
   103741 cctggcccat gcgctgaaaa tgatgcgcga aaacccggaa aaagagcagc tactggtggt
   103801 taacctttcc ggtcgcggcg ataaagacat cttcaccgtt cacgatattt tgaaagcacg
   103861 aggggaaatc tgatggaacg ctacgaatct ctgtttgccc agttgaagga gcgcaaagaa
   103921 ggcgcattcg ttcctttcgt cacgctcggt gatccgggca ttgagcagtc gctgaaaatt
   103981 atcgatacgc taattgaagc cggtgctgac acgctggagt taggcatccc cttctccgac
   104041 ccactggcag atggcccgac gattcaaaac gccacactgc gtgcctttgc ggcaggtgtg
   104101 accccggcgc agtgctttga gatgctggca ctcattcgcc agaaacaccc gaccattccc
   104161 attggcctgc tgatgtatgc caacctggtg tttaacaaag gcattgatga gttttatgcc
   104221 cagtgcgaga aagtcggcgt cgattcggtg ctggttgccg atgtgccagt tgaagagtcc
   104281 gcgcccttcc gccaggccgc gttgcgtcat aatgtcgcac ctatcttcat ctgcccgcca
   104341 aatgccgatg acgacctgct gcgccagata gcctcttacg gtcgtggtta cacatatttg
   104401 ctgtcacgag cgggcgtgac cggcgcagaa aaccgcgccg cgttacccct caatcatctg
   104461 gttgcgaagc tgaaagagta caacgcggca cctccattgc agggatttgg tatttccgcc
   104521 ccggatcagg taaaagcagc gattgatgca ggagctgcgg gcgcgatttc tggttctgcg
   104581 attgttaaaa tcatcgagca acatattaat gagccaaaga aaatgctggc ggcactgaaa
   104641 gcttttgtac aaccgatgaa agcggcgacg cgcagttaat cccacagccg ccagttccgc
   104701 tggcggcatt ttaactttct ttaacgaagc cggaaaaatc ctaaattcat ttaatattta
   104761 tctttttacc gtttcgctta cccctgtcga acgtcaactt acgtcatttt tccgcccaac
   104821 agtaatataa tcaaacaatt taatcccgca acataacacc agtaaaatca ataattttct
   104881 ctaagtcact tattccgcag gcaattgtta atatatcaag aacgttcgtc ataatatatt
   104941 ttcctacttt cttctcgtta cgcttaattt gactaatcct cattagcgac taattttaat
   105001 gagtatcgac acacaacact catattaatg aaacaatgca acgcaacggg agaaataaca
   105061 tggccgaaca tcgtggtggt tcaggaaatt tcgccgaaga ccgtgagaag gcatccgacg
   105121 caggccgtaa aggcggtcag catagcggcg gtaattttaa aaatgatccg caacgcgcat
   105181 ctgaagcggg taaaaaaggc ggtcaacaaa gcggtggtaa taaatcagga aaatcctgat
   105241 tcaccgttaa ttattaatat cagctgataa agataattgc atctgcgggc cgtatggctc
   105301 gcagtgattt cactggagaa aaatatgaat atgaagacca ttgaagatgt atttattcac
   105361 ctgctttcag atacctacag cgcagaaaaa caattaaccc gggcactggc aaaactcgca
   105421 agagcaacat caaacgaaaa attaagtcag gcttttcatg cgcacctcga ggaaacacat
   105481 ggacagattg aacgtattga tcaagttgtg gaatcagaat cgaatctgaa aattaagcgc
   105541 atgaaatgtg tggcaatgga aggtcttatt gaagaagcta atgaggtcat cgaaagtacc
   105601 gagaaaaacg aagtgcgtga tgccgcactg attgccgcag cacagaaagt cgagcattat
   105661 gagattgcca gttacgggac attagcgacg ctggctgaac aattaggtta ccgtaaagca
   105721 gcgaagcttc tgaaagagac cctggaagaa gaaaaggcca ccgacatcaa actgactgat
   105781 ctggccctta ataacgtaaa taagaaagcc gaaaataaag cctgaaatat gaactttaac
   105841 ttttggtcat tttataaaga ggacattttc atgaatcgta ttgaacatta tcatgactgg
   105901 ttacgtgacg cccatgcaat ggaaaagcaa gccgaatcta tgcttgaatc catggccagc
   105961 cgtatagata attatcctga actacgtgct cgtattgaac aacatcttag tgaaaccaaa
   106021 aaccagattg ttcaactgga aactattctt gatcgtaatg acatttcacg ttcagtcatt
   106081 aaagattcca tgagtaaaat ggctgcgctt gggcagtcaa tcggtggtat attcccttct
   106141 gatgaaatag tcaaaggctc tattagcgga tatgtcttcg agcaatttga aatcgcctgt
   106201 tacacctctc tattagcagc agcaaaaaat gccggtgata cagcctcaat tccgatcatc
   106261 gaagcgattt taaatgagga aaagcaaatg gccgactggc tgattcagca tattccgcaa
   106321 acaactgaga aatttttaat tcgctctgaa actgatggcg tagaagcgaa gaaataatac
   106381 cctttatgcc atgtccttat tgaccccgta tattacgggg tcgtttttgt gcggaattaa
   106441 aaacgatatc ctgctgagaa cataaacacc cacggatcga ggcgtacgct atcatgttgc
   106501 tgtgcaccgc ccagtttata attggcggtg gtatcgatat ccatgtacca cactggtagg
   106561 tttcaaaaaa cacaataacc aattgtattt gaagcaaaaa acaagacttt gagatgcttt
   106621 tttgtacctt ttttttatgt ttttaactgc ctgattttat tgaaatcttt tttagttttg
   106681 cagaaggaat tttttacttg attgtaactt tttcgttttt tttggggcta attgacgcgc
   106741 aatgctgggc ggggagcgca cagccaaaaa acccgaaatt gaccgctaat tactgccaat
   106801 catcatcgct ctgattgagc gtaaatcatt caggttattc agttcttcct tctgctctcg
   106861 ctgacgtcaa taaatcccat cattgcgatc aacctcatcc ttaaccattg atgcgagcag
   106921 ttcttccagt ttctgcgctg acagctttac ctggtgatct tcagcatctt cccgggctat
   106981 tgtggttcgc gcagtggctg attttgctga catcaccaca gggggcagac ggaccattga
   107041 accagggcct ccggaacaaa taagggcaaa aataacaacc actatcgaaa gctcacaaac
   107101 cagccgcagc acattcttgc atacaacgtg ctgcggcata accccaatga ttactccata
   107161 taacgtgggg aacatcggct tcgaatagtg ccatgaactg gcaggtatcg aggcagcgcc
   107221 atttccggtg gcggcgatcg tgaacaggac tctgttgccc gcatgccggg catattagta
   107281 gtgtattttt aacgcaccca acaacaacgg tggcaggacc agccctttat caagaaaaag
   107341 tgattcaacc tgctacgtga cgggcaggtt gagaatgtac gcatatagct atttttcatt
   107401 catgaatata gcacttataa atattttctg tttaataatg aataaaaatc atttgtacgc
   107461 atctgtaatc gtcgatcgat aatct
//
LOCUS       DAZFAB010000003       100546 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZFAB010000003 DAZFAB010000000
VERSION     DAZFAB010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 100546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 100546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..100546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.003"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(138..713)
                     /gene="gmhB"
                     /locus_tag="ACHJWA_000228"
     CDS             complement(138..713)
                     /gene="gmhB"
                     /locus_tag="ACHJWA_000228"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFO4306905.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
     gene            901..1932
                     /gene="metN"
                     /locus_tag="ACHJWA_000229"
     CDS             901..1932
                     /gene="metN"
                     /locus_tag="ACHJWA_000229"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFO4306906.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            1925..2578
                     /gene="metI"
                     /locus_tag="ACHJWA_000230"
     CDS             1925..2578
                     /gene="metI"
                     /locus_tag="ACHJWA_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFO4306907.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVVGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            2618..3433
                     /gene="metQ"
                     /locus_tag="ACHJWA_000231"
     CDS             2618..3433
                     /gene="metQ"
                     /locus_tag="ACHJWA_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFO4306908.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKNGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            3551..3955
                     /gene="rcsF"
                     /locus_tag="ACHJWA_000232"
     CDS             3551..3955
                     /gene="rcsF"
                     /locus_tag="ACHJWA_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFO4306909.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQALCIGSALNITAK"
     gene            3952..4659
                     /gene="tsaA"
                     /locus_tag="ACHJWA_000233"
     CDS             3952..4659
                     /gene="tsaA"
                     /locus_tag="ACHJWA_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFO4306910.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDCVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            4770..6488
                     /gene="proS"
                     /locus_tag="ACHJWA_000234"
     CDS             4770..6488
                     /gene="proS"
                     /locus_tag="ACHJWA_000234"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFO4306911.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIKK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFTAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            6541..7365
                     /gene="yaeF"
                     /locus_tag="ACHJWA_000235"
     CDS             6541..7365
                     /gene="yaeF"
                     /locus_tag="ACHJWA_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFO4306912.1"
                     /translation="MDKPKAYCRLLLPCFLLLSACTVDISQPDSSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITVPDLKPGDLLFSSSLEVTSFGIRVFSTSSVSHVAIYLGENN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATEITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVVEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTR"
     gene            complement(7565..8275)
                     /gene="nlpE"
                     /locus_tag="ACHJWA_000236"
                     /gene_synonym="cutF"
     CDS             complement(7565..8275)
                     /gene="nlpE"
                     /locus_tag="ACHJWA_000236"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308221.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFO4306913.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERGYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNQDCSSLGQ"
     gene            complement(8289..8699)
                     /gene="arfB"
                     /locus_tag="ACHJWA_000237"
     CDS             complement(8289..8699)
                     /gene="arfB"
                     /locus_tag="ACHJWA_000237"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFO4306914.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRSGRE"
     gene            complement(8696..9241)
                     /gene="yaeQ"
                     /locus_tag="ACHJWA_000238"
     CDS             complement(8696..9241)
                     /gene="yaeQ"
                     /locus_tag="ACHJWA_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFO4306915.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCAQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            9407..9607
                     /gene="yaeP"
                     /locus_tag="ACHJWA_000239"
     CDS             9407..9607
                     /gene="yaeP"
                     /locus_tag="ACHJWA_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFO4306916.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            9594..9854
                     /gene="rof"
                     /locus_tag="ACHJWA_000240"
     CDS             9594..9854
                     /gene="rof"
                     /locus_tag="ACHJWA_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFO4306917.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(9907..11202)
                     /gene="tilS"
                     /locus_tag="ACHJWA_000241"
     CDS             complement(9907..11202)
                     /gene="tilS"
                     /locus_tag="ACHJWA_000241"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFO4306918.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGGAL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELAQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAETTARSAALCAE
                     QESLLDELLADDLAHCQSPQGTLQIVPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSENIVPWQTWLQPLELPA
                     GQGSVQLNAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGGFVTQEGVAEGENGISFVWQRNA"
     gene            complement(11267..11656)
                     /gene="yaeR"
                     /locus_tag="ACHJWA_000242"
     CDS             complement(11267..11656)
                     /gene="yaeR"
                     /locus_tag="ACHJWA_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO4306919.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            complement(11713..13854)
                     /gene="ldcC"
                     /locus_tag="ACHJWA_000243"
     CDS             complement(11713..13854)
                     /gene="ldcC"
                     /locus_tag="ACHJWA_000243"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFO4306920.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALCF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            complement(13953..14912)
                     /gene="accA"
                     /locus_tag="ACHJWA_000244"
     CDS             complement(13953..14912)
                     /gene="accA"
                     /locus_tag="ACHJWA_000244"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFO4306921.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            complement(14925..18407)
                     /gene="dnaE"
                     /locus_tag="ACHJWA_000245"
     CDS             complement(14925..18407)
                     /gene="dnaE"
                     /locus_tag="ACHJWA_000245"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFO4306922.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQHLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            complement(18444..19040)
                     /gene="rnhB"
                     /locus_tag="ACHJWA_000246"
     CDS             complement(18444..19040)
                     /gene="rnhB"
                     /locus_tag="ACHJWA_000246"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFO4306923.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            complement(19037..20185)
                     /gene="lpxB"
                     /locus_tag="ACHJWA_000247"
     CDS             complement(19037..20185)
                     /gene="lpxB"
                     /locus_tag="ACHJWA_000247"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFO4306924.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NIILEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNSARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAAVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            complement(20185..20973)
                     /gene="lpxA"
                     /locus_tag="ACHJWA_000248"
     CDS             complement(20185..20973)
                     /gene="lpxA"
                     /locus_tag="ACHJWA_000248"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFO4306925.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMAAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            complement(20977..21432)
                     /gene="fabZ"
                     /locus_tag="ACHJWA_000249"
     CDS             complement(20977..21432)
                     /gene="fabZ"
                     /locus_tag="ACHJWA_000249"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFO4306926.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            complement(21537..22562)
                     /gene="lpxD"
                     /locus_tag="ACHJWA_000250"
     CDS             complement(21537..22562)
                     /gene="lpxD"
                     /locus_tag="ACHJWA_000250"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFO4306927.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            complement(22566..23051)
                     /gene="skp"
                     /locus_tag="ACHJWA_000251"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             complement(22566..23051)
                     /gene="skp"
                     /locus_tag="ACHJWA_000251"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFO4306928.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            complement(23172..25604)
                     /gene="bamA"
                     /locus_tag="ACHJWA_000252"
     CDS             complement(23172..25604)
                     /gene="bamA"
                     /locus_tag="ACHJWA_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFO4306929.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            complement(25634..26986)
                     /gene="rseP"
                     /locus_tag="ACHJWA_000253"
     CDS             complement(25634..26986)
                     /gene="rseP"
                     /locus_tag="ACHJWA_000253"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFO4306930.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDSIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            complement(26998..27855)
                     /gene="cdsA"
                     /locus_tag="ACHJWA_000254"
     CDS             complement(26998..27855)
                     /gene="cdsA"
                     /locus_tag="ACHJWA_000254"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO4306931.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            complement(27868..28626)
                     /gene="ispU"
                     /locus_tag="ACHJWA_000255"
                     /gene_synonym="uppS"
     CDS             complement(27868..28626)
                     /gene="ispU"
                     /locus_tag="ACHJWA_000255"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFO4306932.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            complement(28815..30011)
                     /gene="ispC"
                     /locus_tag="ACHJWA_000256"
     CDS             complement(28815..30011)
                     /gene="ispC"
                     /locus_tag="ACHJWA_000256"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFO4306933.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDASAREVARKE
                     VMRLAS"
     gene            complement(30103..30660)
                     /gene="frr"
                     /locus_tag="ACHJWA_000257"
     CDS             complement(30103..30660)
                     /gene="frr"
                     /locus_tag="ACHJWA_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFO4306934.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            complement(30810..31535)
                     /gene="pyrH"
                     /locus_tag="ACHJWA_000258"
     CDS             complement(30810..31535)
                     /gene="pyrH"
                     /locus_tag="ACHJWA_000258"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFO4306935.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            complement(31682..32533)
                     /gene="tsf"
                     /locus_tag="ACHJWA_000259"
     CDS             complement(31682..32533)
                     /gene="tsf"
                     /locus_tag="ACHJWA_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFO4306936.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            complement(32791..33516)
                     /gene="rpsB"
                     /locus_tag="ACHJWA_000260"
     CDS             complement(32791..33516)
                     /gene="rpsB"
                     /locus_tag="ACHJWA_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFO4306937.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            33884..34678
                     /gene="map"
                     /locus_tag="ACHJWA_000261"
     CDS             33884..34678
                     /gene="map"
                     /locus_tag="ACHJWA_000261"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFO4306938.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            34740..37412
                     /gene="glnD"
                     /locus_tag="ACHJWA_000262"
     CDS             34740..37412
                     /gene="glnD"
                     /locus_tag="ACHJWA_000262"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFO4306939.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVNVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEVLHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            37443..38267
                     /gene="dapD"
                     /locus_tag="ACHJWA_000263"
     CDS             37443..38267
                     /gene="dapD"
                     /locus_tag="ACHJWA_000263"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFO4306940.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGDIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            38582..38968
                     /gene="yaeH"
                     /locus_tag="ACHJWA_000264"
     CDS             38582..38968
                     /gene="yaeH"
                     /locus_tag="ACHJWA_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFO4306941.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(39057..40214)
                     /gene="cdaR"
                     /locus_tag="ACHJWA_000265"
     CDS             complement(39057..40214)
                     /gene="cdaR"
                     /locus_tag="ACHJWA_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HFO4306942.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDVEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(40369..41793)
                     /gene="degP"
                     /locus_tag="ACHJWA_000266"
     CDS             complement(40369..41793)
                     /gene="degP"
                     /locus_tag="ACHJWA_000266"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFO4306943.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            complement(41923..43440)
                     /gene="dgt"
                     /locus_tag="ACHJWA_000267"
     CDS             complement(41923..43440)
                     /gene="dgt"
                     /locus_tag="ACHJWA_000267"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFO4306944.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAVL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFSVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRSPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            43524..44222
                     /gene="mtnN"
                     /locus_tag="ACHJWA_000268"
     CDS             43524..44222
                     /gene="mtnN"
                     /locus_tag="ACHJWA_000268"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFO4306945.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            44215..45015
                     /gene="btuF"
                     /locus_tag="ACHJWA_000269"
     CDS             44215..45015
                     /gene="btuF"
                     /locus_tag="ACHJWA_000269"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFO4306946.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATNIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            45053..45676
                     /gene="yadS"
                     /locus_tag="ACHJWA_000270"
     CDS             45053..45676
                     /gene="yadS"
                     /locus_tag="ACHJWA_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFO4306947.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRHLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(45723..46067)
                     /gene="erpA"
                     /locus_tag="ACHJWA_000271"
     CDS             complement(45723..46067)
                     /gene="erpA"
                     /locus_tag="ACHJWA_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFO4306948.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(46060..46125)
                     /gene="yadW"
                     /locus_tag="ACHJWA_000272"
     CDS             complement(46060..46125)
                     /gene="yadW"
                     /locus_tag="ACHJWA_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFO4306949.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            complement(46149..47570)
                     /gene="clcA"
                     /locus_tag="ACHJWA_000273"
     CDS             complement(46149..47570)
                     /gene="clcA"
                     /locus_tag="ACHJWA_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFO4306950.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDVFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            complement(47582..47629)
                     /gene="yadX"
                     /locus_tag="ACHJWA_000274"
     CDS             complement(47582..47629)
                     /gene="yadX"
                     /locus_tag="ACHJWA_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFO4306951.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            47795..49075
                     /gene="hemL"
                     /locus_tag="ACHJWA_000275"
     CDS             47795..49075
                     /gene="hemL"
                     /locus_tag="ACHJWA_000275"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFO4306952.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPD
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            complement(49110..51092)
                     /gene="fhuB"
                     /locus_tag="ACHJWA_000276"
     CDS             complement(49110..51092)
                     /gene="fhuB"
                     /locus_tag="ACHJWA_000276"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFO4306953.1"
                     /translation="MSKRIALFPALLLALLVIAATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGGALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(51089..51979)
                     /gene="fhuD"
                     /locus_tag="ACHJWA_000277"
     CDS             complement(51089..51979)
                     /gene="fhuD"
                     /locus_tag="ACHJWA_000277"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFO4306954.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPESVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMNPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            complement(51979..52776)
                     /gene="fhuC"
                     /locus_tag="ACHJWA_000278"
     CDS             complement(51979..52776)
                     /gene="fhuC"
                     /locus_tag="ACHJWA_000278"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFO4306955.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            complement(52827..55070)
                     /gene="fhuA"
                     /locus_tag="ACHJWA_000279"
     CDS             complement(52827..55070)
                     /gene="fhuA"
                     /locus_tag="ACHJWA_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFO4306956.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLDLYNPVNTDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            complement(55290..57824)
                     /gene="mrcB"
                     /locus_tag="ACHJWA_000280"
     CDS             complement(55290..57824)
                     /gene="mrcB"
                     /locus_tag="ACHJWA_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFO4306957.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTDTWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            complement(58020..60449)
                     /gene="hrpB"
                     /locus_tag="ACHJWA_000281"
     CDS             complement(58020..60449)
                     /gene="hrpB"
                     /locus_tag="ACHJWA_000281"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFO4306958.1"
                     /translation="MSSLPVAAVLPELLTALDYAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPTHQRFDDAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRISQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERATAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAGKWLPEYDWPAVDDESLLATLETWLLPHMAGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            60523..61053
                     /gene="thpR"
                     /locus_tag="ACHJWA_000282"
     CDS             60523..61053
                     /gene="thpR"
                     /locus_tag="ACHJWA_000282"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFO4306959.1"
                     /translation="MSDPQRLFFAIDLPAEIREQIIHWRATHFPLEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWVLTQ"
     gene            61068..61772
                     /gene="sfsA"
                     /locus_tag="ACHJWA_000283"
     CDS             61068..61772
                     /gene="sfsA"
                     /locus_tag="ACHJWA_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFO4306960.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            61950..62405
                     /gene="dksA"
                     /locus_tag="ACHJWA_000284"
     CDS             61950..62405
                     /gene="dksA"
                     /locus_tag="ACHJWA_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFO4306961.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            62472..63368
                     /gene="gluQRS"
                     /locus_tag="ACHJWA_000285"
     CDS             62472..63368
                     /gene="gluQRS"
                     /locus_tag="ACHJWA_000285"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFO4306962.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARHGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHHAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVSEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWRLTAVPESAIVNSTFSNASC"
     gene            63428..64825
                     /gene="pcnB"
                     /locus_tag="ACHJWA_000286"
     CDS             63428..64825
                     /gene="pcnB"
                     /locus_tag="ACHJWA_000286"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFO4306963.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            64822..65304
                     /gene="folK"
                     /locus_tag="ACHJWA_000287"
     CDS             64822..65304
                     /gene="folK"
                     /locus_tag="ACHJWA_000287"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFO4306964.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILTVSSFYRTP
                     PLGPQDQPDYLNAAVALETTLAPEELLNHTQQIELQQGRVRKAERWGPRTLDLDIMLF
                     GNETINTERLTVPHYDMKNRGFMLWPLFEIAPELVFPDGETLREILTSLAVLQPAIWY
                     "
     gene            65585..66181
                     /locus_tag="ACHJWA_000288"
     CDS             65585..66181
                     /locus_tag="ACHJWA_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4306965.1"
                     /translation="MKKALLAAALVMASGSALAVDGGHIDFNGMVQSGTCKVGVVDTG
                     MHSVTTDGVVTLDTANVTDTFAEVSATAVGLLPKEFMISVECDPGAPKNAELTMGSAS
                     YANTSGTLNNNMNITVNGIAPAQNVNIAVHNMKNKAGAAEIKQVHMNNSSEVQELTLD
                     AEGKGQYVFNASYVKAPNSPAVTAGHVTTNALYTVAYK"
     gene            66268..66993
                     /locus_tag="ACHJWA_000289"
     CDS             66268..66993
                     /locus_tag="ACHJWA_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFO4306966.1"
                     /translation="MFPLVKKTVSALFVSTLLASAPAFADIIISGTRIIYNADKKDVN
                     VRLENKGNRPLLIQNWLDTGDDNADPSQIKVPFTATPPVSRVEPKRGQTVKVMYTGAT
                     ALPKDRESVYWFNVLEVPPKPKDAEADKNLLQLAFRTRIKLFYRPSGLQGEPAEAPAK
                     ITWKLNNAQLQANNPTPYYVSFNEVKLESGGKTYNVNSSMVTPFSQASFGVTSLPGSV
                     SSGKVVFKAINDFGGNIDGSATF"
     gene            67035..69635
                     /locus_tag="ACHJWA_000290"
     CDS             67035..69635
                     /locus_tag="ACHJWA_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
                     /protein_id="HFO4306967.1"
                     /translation="MYQFTHQKSRIPKKTLLAACCALFYSSNGAAADTVEYDSSFLMG
                     TGASTIDVKRYAQGNPTPPGLYNVRVFVNGQATSSLEIPFVDIGENSAAACLTHKNLA
                     QLHIKQPEQPVTLLAREGEEEDCLDLAKSYEKADVCFDGSDQFLDLTIPQAYVLKSYG
                     GYVDPSLWESGINAATLAYTLNAYHTSSDNDNSDSVYGAFNSGINLGAWHFRARGNYN
                     WTTDNGSDFDFQDRYLQRDIPAIRSQIIMGDAYTTGETFDSVNVRGVRLYSDSRMLPS
                     ALASYAPTIRGVANSNAKVTVTQSGYKIYETTVPPGEFVIDDISPSGFGSELVVTIEE
                     ADGSKRTFTQPFSSVVQMQRPGVGRWDFSAGKVIDDSLRSEPNMGQASYYYGLNNLFT
                     GYTGIQFTDNNYLAGLLGVGINTSIGAFAVDVTHSRAEIPDDKTYQGQSYRVTWNKLF
                     QDTGTSFNLAAYRYSTQDYLGLHDALVLIDDAKHLSADEDKNTMQTYSRMKNQFTVSI
                     NQPLNIAYEDYGSLFISGSWTYYWAANNSRTEYNVGYSKSVSWGSFSVNLQRSWNEDG
                     EKDDAMYVSVSVPIENILGGKRKSSGFRNLNTQLNTDFDGSHQLNVNSSGNTENNLVN
                     YSVNAGYSLDKNAGDLASVGGYLNYESGLGGISASASATSDNSQQYSISTDGGFVLHS
                     GGLTFTNNSFSSNDTLVLINALGAKGARINNSNNEIDRWGYAVTSSVSPYRENRVGLN
                     IETLENDVELKSTSATTVPRSGSVVLTRFETDEGRSAVLNITAANGKSIPFAAEVYQG
                     EVMIGSMGQGGQAFVRGINDSGELIVRWYENNQTIDCKLHYQFPAQPQTQGSTNTLLL
                     NNLTCQVANH"
     gene            69649..70197
                     /locus_tag="ACHJWA_000291"
     CDS             69649..70197
                     /locus_tag="ACHJWA_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4306968.1"
                     /translation="MKRILLTSALIGLGLPAVGSATDLNVDFTATVLATTCTITIVED
                     GGPAVTGSNDEYSLTIPDVGLDKVATAAPEAQANFKLKASDCSNGYSKIFTTLTGTTV
                     SGKLIVNEATSGAAGVGMGIKRRDTADSTFFTPNNTDKFEWSADEKASGVPLTVALRE
                     TTAGAGRTGAFQAKATFNFTYE"
     gene            70212..70817
                     /locus_tag="ACHJWA_000292"
     CDS             70212..70817
                     /locus_tag="ACHJWA_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4306969.1"
                     /translation="MKLKVIATLIATVAVGVSFNSNFASASTTSASLTVNSNLTMGTC
                     SAQIMDNSNKVINEVVFGNVYISELGAKSKVQQFKIRFSNCSGLPQNSAQIVLAPNGI
                     SCAGSQSSSAGFSNKFTDASAATRTAVEVWTTDTPESNGSTQFHCAQKIPVPVTLPAD
                     TTTQPYDYPLSARMTVAEGRLVTDVRPGNFRSPTTFTITYQ"
     gene            70838..71428
                     /locus_tag="ACHJWA_000293"
     CDS             70838..71428
                     /locus_tag="ACHJWA_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFO4306970.1"
                     /translation="MLCRHHKIVHFLGLATALITPFAYSGQDVDLTAKIVPSTCQVEV
                     SNNGVVDLGTVTLDYFADNVTPTTDYAGGKTFNVNVVSCDNIQTTQSQMKLDFQPQAG
                     SLAQANNQIFSNEYEQQATGAKNVGIVIFSAQPNQQTFNVRGTDGSSTAIYSVAPGNA
                     VPSTWTFYSRMQRVNNALPPESGMVRSQVIVNVSYE"
     gene            71440..72549
                     /locus_tag="ACHJWA_000294"
     CDS             71440..72549
                     /locus_tag="ACHJWA_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4306971.1"
                     /translation="MQRKGNKLLIQLCSVILLFFTTSWYALANECYIERNAEGDYHMK
                     ISSTQLSLASQMVEVPTEIAEATWDVNIQLRGDAIGCKSLGDSKAVHFLNTADPSLIS
                     TYTTTNGAALLKTTVPGIVYSVELLCLSCGAADELDLWLPAQSGADNFIPSTQTKWAY
                     EYSDQSWYLRFRLFITPEFKPKNGVSSGTTIAGKIASWYIGTNDQPWINFYIDNDSLK
                     FFVDEPTCATVALAQDQGNVSGNQVTLGNSYVSEVKNGLTREIPFSIRAEYCYASKIT
                     VKLKAANKPSDATLVGKTTGSASGVAVKVNSTYDNSKVLLKADGSNTVDYNFAAWSNN
                     LLFLPFTAQLVPDGSGNAVGVGTFSGNATFSFTYE"
     gene            72684..73478
                     /gene="panB"
                     /locus_tag="ACHJWA_000295"
     CDS             72684..73478
                     /gene="panB"
                     /locus_tag="ACHJWA_000295"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFO4306972.1"
                     /translation="MKPTTIASLQKCKQEKKRFATITAYDYSFAKLFAEEGLNVMLVG
                     DSLGMTVQGHESTLPVTVEDIAYHTTAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            73490..74341
                     /gene="panC"
                     /locus_tag="ACHJWA_000296"
     CDS             73490..74341
                     /gene="panC"
                     /locus_tag="ACHJWA_000296"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFO4306973.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(74640..75536)
                     /gene="rpnC"
                     /locus_tag="ACHJWA_000297"
     CDS             complement(74640..75536)
                     /gene="rpnC"
                     /locus_tag="ACHJWA_000297"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFO4306974.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSESQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFAQRFLSKG
                     MSREDVAEMTNLSLAEIDRLIN"
     gene            75810..76190
                     /gene="panD"
                     /locus_tag="ACHJWA_000298"
     CDS             75810..76190
                     /gene="panD"
                     /locus_tag="ACHJWA_000298"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFO4306975.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(76194..77423)
                     /gene="yadE"
                     /locus_tag="ACHJWA_000299"
     CDS             complement(76194..77423)
                     /gene="yadE"
                     /locus_tag="ACHJWA_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFO4306976.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFDKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMVQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKW
                     EPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(77487..77927)
                     /gene="yadI"
                     /locus_tag="ACHJWA_000300"
     CDS             complement(77487..77927)
                     /gene="yadI"
                     /locus_tag="ACHJWA_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO4306977.1"
                     /translation="MLGWVITCHDDRAQEILDVLEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGAPEVSSIWHQQQKNPPFVLKHNLYEY"
     gene            complement(78032..78802)
                     /gene="yadH"
                     /locus_tag="ACHJWA_000301"
     CDS             complement(78032..78802)
                     /gene="yadH"
                     /locus_tag="ACHJWA_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4306978.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(78799..79725)
                     /gene="yadG"
                     /locus_tag="ACHJWA_000302"
     CDS             complement(78799..79725)
                     /gene="yadG"
                     /locus_tag="ACHJWA_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO4306979.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            79834..80496
                     /gene="can"
                     /locus_tag="ACHJWA_000303"
     CDS             79834..80496
                     /gene="can"
                     /locus_tag="ACHJWA_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFO4306980.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLDEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(80537..81073)
                     /gene="hpt"
                     /locus_tag="ACHJWA_000304"
     CDS             complement(80537..81073)
                     /gene="hpt"
                     /locus_tag="ACHJWA_000304"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFO4306981.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            81279..83669
                     /gene="gcd"
                     /locus_tag="ACHJWA_000305"
     CDS             81279..83669
                     /gene="gcd"
                     /locus_tag="ACHJWA_000305"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFO4306982.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(83871..85421)
                     /gene="cueO"
                     /locus_tag="ACHJWA_000306"
     CDS             complement(83871..85421)
                     /gene="cueO"
                     /locus_tag="ACHJWA_000306"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFO4306983.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVNELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHDAPKERAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            85587..85934
                     /gene="yacC"
                     /locus_tag="ACHJWA_000307"
     CDS             85587..85934
                     /gene="yacC"
                     /locus_tag="ACHJWA_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFO4306984.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            86040..86906
                     /gene="speE"
                     /locus_tag="ACHJWA_000308"
     CDS             86040..86906
                     /gene="speE"
                     /locus_tag="ACHJWA_000308"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFO4306985.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAVHTAAFALP
                     QYLQDALASQPS"
     gene            86922..87716
                     /gene="speD"
                     /locus_tag="ACHJWA_000309"
     CDS             86922..87716
                     /gene="speD"
                     /locus_tag="ACHJWA_000309"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFO4306986.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(87754..88116)
                     /gene="yacL"
                     /locus_tag="ACHJWA_000310"
     CDS             complement(87754..88116)
                     /gene="yacL"
                     /locus_tag="ACHJWA_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFO4306987.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(88292..90889)
                     /gene="acnB"
                     /locus_tag="ACHJWA_000311"
     CDS             complement(88292..90889)
                     /gene="acnB"
                     /locus_tag="ACHJWA_000311"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFO4306988.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAVAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            91244..93097
                     /gene="yacH"
                     /locus_tag="ACHJWA_000312"
     CDS             91244..93097
                     /gene="yacH"
                     /locus_tag="ACHJWA_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFO4306989.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVETQSTQ
                     LAVSDAAAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDALLSQVLMASTY
                     PTNVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKTVPVTQTVTAPVIPSN
                     TVSTANPVITEPATTVISIEPGNPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDNYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVHEKMQTNPQNQQRREAARERIQPASPEQRQ
                     AVREKMQTNPQIQQRRDAARERIQSASPEQRQVFKEKVQQRPLNQQQRDNARQRVQSA
                     SPEQRQVFREKVQESRPQRLNDSNHTARLNNEQRSAVRERLSERGARRLER"
     gene            complement(93168..94592)
                     /gene="lpdA"
                     /locus_tag="ACHJWA_000313"
     CDS             complement(93168..94592)
                     /gene="lpdA"
                     /locus_tag="ACHJWA_000313"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFO4306990.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(94800..96692)
                     /gene="aceF"
                     /locus_tag="ACHJWA_000314"
     CDS             complement(94800..96692)
                     /gene="aceF"
                     /locus_tag="ACHJWA_000314"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFO4306991.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAVPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNTGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(96707..99370)
                     /gene="aceE"
                     /locus_tag="ACHJWA_000315"
     CDS             complement(96707..99370)
                     /gene="aceE"
                     /locus_tag="ACHJWA_000315"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFO4306992.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(99531..100295)
                     /gene="pdhR"
                     /locus_tag="ACHJWA_000316"
     CDS             complement(99531..100295)
                     /gene="pdhR"
                     /locus_tag="ACHJWA_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFO4306993.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
BASE COUNT        24370 a        27323 c        24850 g        24003 t
ORIGIN      
        1 attttttctc ttcaaccgaa ccggctgttt gtgtgaagtg attcacattc gccgtgtcga
       61 tggaggcgca ttatagggag tcggctcagg aagacaagcg gaaaaatgca tttttatttc
      121 aaccgctcat cttttaatca ttgcgccggt ttttgctgct tttttatcgc ttgcggcagg
      181 tctgccaggc tatttaacac ccaatctgcc gcgttttctg cttcaggtgt aataggttta
      241 cccgtacgca ccagcacttt tgttcccacg ttcgccgcaa ccgctgcctg catatcttct
      301 aatttatcgc ccaccatata agaagcggcc atatcaatat gcaaataatc gcgtgctgac
      361 aaaagcatcc ccggatgtgg tttgcggcaa tcgcagacct ggcgaaactc ttcaacacta
      421 ccctgcggat gatgcgggca ataatagata ccatccagat cgacatctcg gtccgccagc
      481 gaccagtcca tccactcggt cagcgtttca aactgtgctt cggtaaattt accgcgagca
      541 atgccagact ggttggtgac taccaccagc gcaaagccca ttttttttag ctcgcgcatg
      601 gcgtcaataa caccgtcgat aaattcaaag ttgtcgatct catggacata gccgtgatcg
      661 acattaatgg tgccatcacg gtcaagaaaa attgcgggta cgctcttcgc caccttttat
      721 agctccttaa taaggcatgt gacgctagta tcgcatgttt cgacctgcaa gaaagtgctc
      781 ttcgcataaa cctgattgat ttagacgtct ggatgcctta acatccattt cattgacggc
      841 gttgcccgtt tcaggcattc gagatgccac gactaactta atgacgataa taaataatca
      901 atgataaaac tttcgaatat caccaaagtg ttccaccagg gcacccgcac catccaggcg
      961 ttgaacaacg tcagcctgca tgtgccagct ggacaaattt atggcgttat cggtgcctca
     1021 ggcgcgggta agagtacgct tatacgttgt gtaaacctgc tggagcgccc aaccgagggt
     1081 agcgtgctgg tcgatggaca ggaactgacc acgctgtcag aatccgagtt gaccaaagct
     1141 cgccgccaga ttggtatgat tttccagcat tttaacctgc tctcttcgcg tactgttttt
     1201 ggcaacgtgg ctctgccgct ggagctggac aatacaccga aagacgagat caaacgtcgc
     1261 gtgacggaat tgctgtcatt ggttggtctt ggagataagc atgatagcta cccgtcgaat
     1321 ctttccggtg ggcagaaaca acgtgtggcg attgcccgtg cattagccag caatcccaaa
     1381 gtattgctgt gtgatgaagc caccagcgcg ctagacccgg caacgacacg ttctattctc
     1441 gaactgctga aagacattaa ccgccgtctg ggtttgacga ttctgttgat cacccacgaa
     1501 atggacgttg tgaagcgtat ttgtgattgc gttgcggtca tcagcaatgg cgaactgatc
     1561 gagcaggaca cggtaagtga agtgttctca catccgaaaa cgccgctggc gcagaagttt
     1621 attcagtcaa ccctgcatct ggatatcccg gaagattacc aggaacgtct gcaagcggag
     1681 ccatttactg actgcgtgcc gatgctgcgt ctggagttta ccggtcaatc ggtcgatgcc
     1741 ccactgcttt ctgaaaccgc gcgtcgtttc aacgtcaaca acaacattat tagcgcgcag
     1801 atggattacg ccggtggcgt taagttcggc atcatgctga ctgaaatgca cggcacacaa
     1861 caagatacgc aagccgccat tgcctggctg caggaacacc atgtaaaagt agaggtactg
     1921 ggttatgtct gagccgatga tgtggctgct ggttcgtggc gtatgggaaa cgctggcaat
     1981 gaccttcgta tccggatttt ttgggtttgt ggttggtctg ccggttggcg tcctgcttta
     2041 tgtcacgcgt ccggggcaaa ttattgctaa cgcgaagctg tatcgtaccg tttctgcgat
     2101 tgtgaacatt ttccgttcca tcccgttcat tatcttgctg gtatggatga ttccgtttac
     2161 ccgcgttatt gtcggtacat cgattggttt gcaggcagca attgttccgt taaccgttgg
     2221 tgcagcaccg tttattgccc gtatggtcga gaacgctctg ctggagatcc caaccgggtt
     2281 aattgaagct tcccgcgcaa tgggtgccac gccaatgcag atcgtccgta aagtgctgtt
     2341 accggaagcg ttgccgggtc tggtgaatgc ggcaactatc accctgatta ccctggtcgg
     2401 ttattccgcg atgggtggtg cagtcggtgc cggtggttta ggtcagattg gctatcagta
     2461 tggctacatc ggctataacg cgacggtgat gaatacggta ctggtattgc tggtcattct
     2521 ggtttattta attcagttcg caggcgaccg catcgtccgg gctgtcactc gcaagtaacg
     2581 ttcaacacaa cataaataat tgaagaagga ataaggtatg gcgttcaaat tcaaaacctt
     2641 tgcggcagtg ggagccctga ttggatcact ggcactggta ggctgcggtc aggatgaaaa
     2701 agatccaaac cacattaaag tcggcgtgat tgttggtgcc gaacagcagg ttgcagaagt
     2761 cgcgcagaaa gttgcgaaag acaaatatgg cctggacgtt gagctggtaa ccttcaacga
     2821 ctatgttctg ccaaacgaag cattgagcaa aggcgatatc gacgccaacg ccttccagca
     2881 taaaccgtac cttgatcagc aactgaaaga tcgtggctac aaactggtcg cagtaggcaa
     2941 cacttttgtt tatccgattg ctggttactc caagaaaatc aaatcactgg atgaactgca
     3001 ggatggttcg caggttgccg tgccaaacga tccaactaac cttggtcgtt cactgctgct
     3061 gctgcaaaaa gtgggcttga tcaaactgaa agatggcgtt ggcctgctgc cgaccgttct
     3121 tgatgttgtt gagaacccaa aaaatctgaa aattgttgaa ctggaagcac cgcaactgcc
     3181 gcgttctctg gacgacgcgc aaatcgctct ggcagttatt aacaccacct atgccagcca
     3241 gattggcctg actccagcga aaaacggtat ctttgtcgaa gataaagact ccccgtacgt
     3301 aaacctgatc gtaacgcgtg aagataacaa agacgccgag aacgtgaaga aattcgtcca
     3361 ggcttatcag tctgacgaag tttacgaagc agcaaacaaa gtgtttaacg gcggcgctgt
     3421 taaaggctgg taatttttag gctgtttcca caatttgtaa tatcattcag gacgggcgct
     3481 tgcccgtctt gtcattttta caagctcctg attcaatatt gacgttttga tcatacattg
     3541 aggaaatact atgcgtgctt taccgatctg tttagtagca ctcatgctaa gcggctgttc
     3601 catgttaagc agatcccctg tcgaacccgt tcaaagcact gcaccccagc cgaaagcgga
     3661 gcctgcaaaa ccgaaagcgc cgcgcgccac gccggtccga atttatacca atgcagaaga
     3721 attagtcggc aaaccgttcc gcgatctcgg tgaagtcagt ggcgactctt gccaggcctc
     3781 taatcaggac tctccgccga gcattccaac cgcgcgtaag cggatgcaaa tcaacgcctc
     3841 gaaaatgaaa gccaatgcgg tattactgca tagctgcgaa gttaccagcg gtacgccagg
     3901 ctgctatcgt caggctttat gtatcggttc tgcgcttaac attacggcga aatgagcagt
     3961 ttccagttcg agcaaatagg tgttattcgc tcgccctata aagaaaaatt cgccgttccg
     4021 cgccagccag gtctggtaaa aagcgccaac ggcgaactac atctcattgc tccctacaac
     4081 caggctgacg ccgttcgcgg cctggaagca ttcagccatt tatggatcct tttcgtcttt
     4141 catcaaacga tggaaggcgg ctggcgtccg actgtgcgtc cgcctcgtct tggcggtaat
     4201 gccagaatgg gtgttttcgc tacgcgctcc actttccgcc ccaacccgat tggtatgtcg
     4261 ctggtagagc tgaaagaggt tgtttgccat aaagactgcg tgattctgaa gctcggcagc
     4321 ctggatctgg tcgatggtac gccggtagtg gatatcaaac cgtatctccc ctttgccgaa
     4381 tcgcttcccg atgccagtgc cagctatgcg caaagtgcgc cagctgcaga gatggcggtg
     4441 agttttaccg cagaggtcga aaagcagctt ttgacgctgg agaagcgtta tccgcagtta
     4501 acgctgttta tccgcgaagt actggcgcag gacccgcgcc cggcctatcg taaaggtgag
     4561 gaaacgggca aaacctatgc cgtctggcta catgatttta acgttcgctg gcgcgtcacc
     4621 gacgcaggtt ttgaagtctt tgcgctggaa ccgcgttaaa ttcacgccct tctcttttga
     4681 catttctttt gcactggtaa actaaatcac tttttttgtc ccaggctcgc cttgagcctg
     4741 ttcgaccttc caactggaac cgtaacaaca tgcgtactag ccaatacctg ctctccactc
     4801 tcaaggagac acctgccgac gccgaggtga tcagccatca gctgatgctg cgcgccggga
     4861 tgatccgcaa gctggcctcc gggttatata cctggctgcc gaccggcgtg cgcgttctga
     4921 aaaaagtcga aaacatcgtg cgtgaagaga tgaacaacgc cggtgcaatc gaggtgtcga
     4981 tgccggtggt tcagccagcc gatttgtggc aagagagtgg tcgttgggaa cagtacggtc
     5041 cggaactgct gcgttttgtt gaccgtggcg agcgtccgtt cgtactcggc ccaactcatg
     5101 aagaagttat cactgacctg attcgtaacg agcttagctc ttacaaacag ctgccgctga
     5161 acttctatca gatccagacc aagttccgcg acgaagtgcg tccgcgtttc ggcgtcatgc
     5221 gttcccgcga attcctgatg aaagatgctt actctttcca tacttctcag gaatccctgc
     5281 aggaaaccta cgatgcaatg tatgcggcct acagcaaaat cttcagccgc atggggctgg
     5341 atttccgcgc cgtacaggcc gacaccggtt ctatcggcgg cagcgcctct cacgaattcc
     5401 aggtgctggc gcagagcggt gaagacgatg tggtcttctc cgacacctct gactatgcag
     5461 cgaacattga gctggcagaa gctatcgcgc cgaaagaacc gcgcgctgct gctacccagg
     5521 aaatgacgct ggttgatacg ccgaacgcga aaaccatcgc ggaactggtt gaacagttca
     5581 atctgccgat taagaaaacg gttaagactc tgctggttaa agcggttgaa ggcagtagct
     5641 tcccgctggt tgcgctgctg gtgcgcggtg atcacgagct gaacgaagtt aaagcagaaa
     5701 aactgccgca ggttgccagc ccgctgactt tcgcgaccga agaagaaatt cgtgccgtgg
     5761 ttaaagccgg tccgggttca ctgggtccgg taaacatgcc gattccggtg gtgattgacc
     5821 gtaccgttgc ggcgatgagt gatttcactg ctggtgctaa catcgatggt aaacactact
     5881 tcggcatcaa ctgggatcgc gatgtcgcta ccccggaagt tgcggatatc cgtaacgtgg
     5941 tggctggcga tccaagcccg gatggccagg gtacgctgct gatcaaacgt ggtatcgaag
     6001 ttggtcacat cttccagctg ggtaccaagt actccgaagc actgaaagcc tccgtacagg
     6061 gtgaagatgg ccgtaaccaa atcctgacta tgggttgcta cggtatcggg gtaacgcgcg
     6121 tagtggctgc ggcgattgag cagaactacg acgagcgcgg catcgtatgg cctgacgcta
     6181 tcgcgccgtt ccaggtggca attctgccga tgaacatgca caaatccttc cgcgtacaag
     6241 agcttgctga gaaactgtac agcgaactgc gtgcacaagg tatcgaagtg ctgctggatg
     6301 accgaaaaga acgtccgggc gtgatgtttg ccgatatgga actgatcggt attccgcaca
     6361 ctattgtgct gggcgaccgt aacctcgaca acgacgatat cgaatataaa tatcgtcgca
     6421 acggcgagaa acagttaatt aagactggtg acatcgtcga atatctggtg aaacagatta
     6481 aaggctgatg ccagaaaggg tcctgaattt cagggccctt ttttacatgg attgtataaa
     6541 atggataaac caaaggcgta ctgccgcctg cttctcccct gctttctttt actttcggcc
     6601 tgtaccgttg atatcagcca gccagactca tcagccacag cagtagatgc cgaggcgaaa
     6661 acatgggctg tcaaattcca gcatcaaagc tctttcaccg aacaatcaat aaaagaaata
     6721 acggtacctg atctcaaacc cggcgatttg ctgttctcct caagcctgga ggtaacctca
     6781 tttgggatcc gcgttttcag cacttcctct gtgagtcacg ttgcaatcta tctgggtgaa
     6841 aataacgttg cagaagcgac aggcgctggc gtccagattg tttcccttaa aaaagcgatg
     6901 aagcatagtg ataaactttt cgtcttacga gtcccggatc ttaccccgca acaagccaca
     6961 gaaatcaccg cgttcgccaa taaaatcaaa gatagcggtt ataactatcg cggcattgtc
     7021 gaatttattc cctttatggt gactcgccag atgtgctcac tgaatccgtt ctccgaggat
     7081 tttcgccaac agtgcgtcag cggcctggcg aaagcgcagt taagcagtgt ggtcgaagga
     7141 gacaaaaagt cgtggttttg ttcggaattt gtcactgatg catttgccaa agccgggcat
     7201 ccgcttacgc tggcgcaatc gggctggatt agccctgccg atctgatgca tatgcgtatt
     7261 ggtgatgtct ctgcgtttaa gccagaaacg cagttacagt atgttgggca cctgaagcca
     7321 gggatttata tcaaggcggg tcgctttgtt ggtttgaccc ggtgataaag taaatgcctt
     7381 atccgcctta aggtatgagg ggggttgtag gcatgataag acgcgccagc gtcgcatcag
     7441 gcgttgagca ccattgccgg atgcggcgta aacgccttat ccggcctaca aaaaacgtgc
     7501 aaattcaaaa tgttgaaata tatccttctg gcctgttttg cgtttgtttc tgtctcaaga
     7561 cgggttactg ccccaaacta ctgcaatcct ggtttgggta aaatttccct gccgtatcgg
     7621 gtgctaatac tttggttggc gcaccggtat ccggattagc ctcaagcgta aagtgacctt
     7681 ctactgacag taacaccggt ttttcactgt ggccgcgcgc agccaggtag ccacgctcca
     7741 gctctgcgtt attcgctacc atgaaacgtt ttccggtcgc gcaatcagtg aaggtcgccg
     7801 catcagccat ataaaaatac atgccccgca gggtcatcgg cgtcatgggt aaactggatt
     7861 gtgccggttc cagcgtatag ttgaactgcg attcaatcgg attgccttca cgatcgagca
     7921 tctccagcgc atcgcctttc gcccgataat atgacttttc acctttgctg tcggttaata
     7981 ccagcttgtc agcggttcgc gcccatgtac cgtaggaagc gaaggaggaa ggttcttcac
     8041 gagcaccgag ataacgctca ttcatcaccc atgttccgtc tttttcgagg aacagagagg
     8101 tttcgattcc ttcgcaatcg gcacacggca gcacgccgcg ccaactttgc ggcatcggtt
     8161 tcaattcggc agcctgcgcc ggagaaagcg tatcgacttc ggcccgatta ttacaaccca
     8221 tcagagtaaa gaggctgatt acagccatcg ctgtcactat cgcttttttc accatccatt
     8281 ccttcttttt attcccgacc gctgcgcatc gccttcacgc ttgatttttg tgctttcgat
     8341 gccagcctgc gctctttcga tgcacgggtg ggccgcgtgg gtcgtcgggc tttttgttct
     8401 gttgttaaat ctttaatcac tgccaccagc cgggccagcg ccgcttcgcg gttcagttcc
     8461 tgactgcggt attcctgtgc cttaatgaca attacgccat cactgctgat caaatgatgg
     8521 ctggcggcga gcagacgctc tttgtaatac tctggcaggc tggatgcccg aatgtcaaaa
     8581 cgcagatgaa tggccgttga ggtcttatta acatgctgcc cgcccgcgcc ctgcgcacga
     8641 atggcggtga tctcaagctc accatcggga atagcaacat gtcgggaaat cacaatcatg
     8701 aaggttgttg ccaggcggtt aagttcactt ccagattatt cttatcatcc gataaccaga
     8761 tcacgccatc ctgaatcgtt gcctgcagcg tcatggtacg atcggcaaag gcgctcactt
     8821 tcgccagctg ctcgtcgtcc agataccaga cggaaagatt ggcaaactgc gcacatttgc
     8881 tctgattttg ctgccaccag atttgcgccg cccgactatt ataggtaaac agcgccactt
     8941 ctgcggcctg ggtgcaggct ttcttaattc gtcgctcatc cggcagcccc agctcaatcc
     9001 acaaatcaat gcccaggtga tcgttacgca gccacgcttc cggctcatca tcggcacaca
     9061 aaccacgggt aaattgcaga cgttcatcag catatttcag ccacgccagc aggcgcagca
     9121 tcatacgctc ctgggtttct gaagggtggc gcgccagcgt cagagaggca tcgagaaact
     9181 ggttgcggtc gagatcggcc acattaaccg tcgctttata aattgtcgct ttaagcgcca
     9241 taagaaaact cctttcgaat caggaggcca ttgtaacgga atacgtgcag ttgcggcgct
     9301 gatattgcag aaatgagtgt ggtatagtca ccttgcgaaa cagagtttat cttcgtaggc
     9361 ttagacttgc atccacggtt aagtcagagt gctgacagga gggcatgtgg aaaaatattg
     9421 tgagttaata cgcaagcggt acgcggaaat cgccagcgga gacttaggat acgttccgga
     9481 cgcgctgggc tgcgttttga aagtgctgaa tgaaatggct gccgacgacg ccctttctga
     9541 ggctgtcagg gaaaaagcag catacgctgc ggcaaactta ctggtgagcg attatgtcaa
     9601 tgaatgatac gtatcaacca atcaattgtg atgattacga taatcttgag ctcgcctgcc
     9661 agcatcattt aatgctgaca cttgagctga aagatggcga aaaattgcag gccaaagcca
     9721 gcgatttagt ctcccgcaaa aatgtggagt atctggtcgt cgaggccgct ggcgaaaccc
     9781 gtgagctacg tctggataaa attaccagct ttagccaccc ggaaatcggt acggtggtgg
     9841 tgagcgagtc ctgattgact ttcgccggat gcgacgctta atgcgtcata tccggccttc
     9901 actaaactaa gcgtttcgct gccagacaaa acttatgcca ttctcacctt cagccacacc
     9961 ttcttgcgtc acaaatcccc ctgccgccgc aatcagcgtt tcgccataaa acagcagtgg
    10021 cgtggtgtca cgtagccacg gcggcacgcc cagctcttgc cagattttct ttagcttacg
    10081 tccgccgtta cgcccgacaa tatgcagcaa tcctggcgct ttgaaacgca cgctgaccgc
    10141 ttcgtctgca cgcggagggc gaatatcgcc tcccgcatta agctgtacac ttccctgccc
    10201 cgccggtaat tccagcggtt gaagccatgt ctgccacggc acaatgtttt cgctttgccc
    10261 ggtgacggat ttaatccacc acagttgcga ctgatagcgt cggatttcaa acgcgcccaa
    10321 acgtaaacag ggggaggcat cttcccgcgc cagcgccact tcctgccaga tcctcaccaa
    10381 cgcgtcgcgg gaaggcatcg gtgcattctg ccctgccagc cagcggcgga taatcgccgc
    10441 gcggcgggca tcactcatcg ccagcattgg cacaatctgc agcgtcccct gcggcgattg
    10501 acagtgtgct aaatcatctg ccagcagttc atccagcagg ctctcttgtt cagcacaaag
    10561 tgcggcgctg cgggccgttg tttcggcaaa atgcggccaa cgctgctgca ataacggcac
    10621 tacgcgcagg cgcagaaagt tacgatcgta tgagtcgtcc tgattacttt cgtcttcaat
    10681 ccagcgtaaa ccatgcgcca atgcccactg cgccagttcc ccccgcgtgc gggcaagcaa
    10741 cgggcggata agccgtgttc cggcaaactc cgaaacttcc gccatagccg aaagcccggc
    10801 agggccgcta ccgcgtttta gcgccagcag aaaagtttca cattgatcgt cgagatgttg
    10861 cgcggtgacc agcacttcac cgggcaacaa ggtgcgggca aatgcctgat aacgcgcctg
    10921 ccgcgcctgg gcctcaatcc ccagcccctc ttgcgcaagt tgtacgcgtt cgaccaccag
    10981 cggcacctgc cactgttggc agacgttttc gcaatgcgta acccaggcat cggcattggc
    11041 acttaaaccg tgatgcacat gaatagcgcg cagagcgccc cccggatttt ccgtccgcca
    11101 ctgcaccaac tgatgcagca gaacggtgga gtcaagcccg ccgctaaagg ccaccagaat
    11161 ctggcgtgag gtgagaagtt gtctattgag cgtgagtgtc atgatggtgc gattttacag
    11221 tagcaatgcc cggcacgtta ccgggcaaat gcggcgatga caagccttac tgctcataca
    11281 gttccaacgg cagcccgtcc ggatcgttaa agaaggtgaa gcgtttttgc gtgtatggat
    11341 cgacacggat ggcttcacac ttcacgttat ggctttcaag gtgcgccact gccgcatcga
    11401 tgtcatcaac gctaaaagcc agatgacgca ggccgcaagc ttccggtcgg ctggggcgct
    11461 ccggcgggaa cggaaatgag aaaagctcaa tcacatattg cccattaagc gccaaatccc
    11521 ctttccacga gtcgcgcgct tcgcgataga cttcgctttg cagcgtgaaa ccaagaatat
    11581 cgcagtagaa agctttgctc accgcataat ccgtcgcaat aatcgcaata tggtgaacct
    11641 gttttaaacc cagcataacg tctcctttat ttgttaacag cacgttactc gcccggaagc
    11701 cgctctggca agttatcccg ccatttttag gactcgtacg cggtaaacgc cgtcttcgtc
    11761 ctgtttcgca ccgtgaatat ccgtttcaaa accggggtaa tgttgcccta cggaacaaag
    11821 catcagtaga aaatcgagta ctgtgcggct ctctttggtc agcatttctc caggcatcaa
    11881 cagcggtacg cccggtggat aaggcaggat catatttgcc gatactctac cgaccagttg
    11941 ttccagcgca atggtttcta cttcaccttt aatctgtctc tgccatgcct gatgtggcgt
    12001 catgatcatc tccggcaaag tatcgaatgc ccgcaacatc aaaccgggaa gatcgtgttt
    12061 acgaatcagc ttatggatcc cttgcgccag atcctgaata cgcatattgc ggtagaaatc
    12121 gggatcttct gcgtacagat ccggaagcat atttttgatt cgcagattga gatcgtaaga
    12181 gcgtttaaat tccgtcaacc cacgcaataa tcccattgct ttggttttat cgatgccaat
    12241 actaaagaga aacagcaggt tatatgggcc ggttttctct accacgatcc cacgctcatc
    12301 gaggaacttc gccaccagcg ccgccgggat cccctcctcg ctcatattgc cctgctcgtc
    12361 catccccggc gtcagaatag tgactttaac cggatcgagg aacatatgat cggcatccgc
    12421 atcgttaaag ccgtgccatt gttcgccagg cgcaacgggc cagcattcgg cttcatccac
    12481 ctgcggcggt tgccagatat cgaaaaacca accgtcagac tcttcccgca gccgctggac
    12541 ctctttgcga aaatgcagag ctcgttctac tgaacggtta atcagccgtt tgcccggatt
    12601 accacgcagc atcgctgctg ccgtctcaac cgaagcaaca atgggataac tgggcgaggt
    12661 ggtggtatgc atcataaagg cttcgttaaa cgcctcttcg tcatactcac ctttaatgtg
    12721 gatcagcgaa gcctgcgata acgccgccag cattttgtgg gtcgattgcg tttcgaagat
    12781 cacttttccc gcaacacgct cgccgctcat accacttttc ccctggtaga tcggatgaaa
    12841 atgggtgtac ggcacccagg cggaatcgaa gtggatcgac gggacatcca gcgtctgttt
    12901 gatccagtcg gtgttgtaga gcaagccatc ataggttgag ttggtaatca ccgcatgaac
    12961 cggccattgt gcctgcgtgg tggcagcgac tttctcttcg atgctgtcgc gagtaaattc
    13021 acggcgcggg atcccaccca gaatccccaa cgcattacgc gtcggtttca gccagactgg
    13081 cactacatcg ttcatcatca acagatgcgc cagcgattta tgacaattgc ggtcaatcaa
    13141 cagcgtactg ccggatggcg cggcgtacat acccacaatt ttgttcgacg tcgatgttcc
    13201 gttggtaacg atataactct gttccgcgcc gaaagtccgc gcgatgtact cttccgcttc
    13261 caggtgtggc ccggtgtggt cgagcaacga accaagctcg gtgaccgaaa tagagacatc
    13321 agccttaagg gtattcccgc cgaaaaagtc ataaaacaga cagccaaccg ggcttttttg
    13381 atatgcggtg ccgcccatat gacccggcgt acaaaaggtg tacttccgct ctttgacgta
    13441 ggtaaacaag gctttcgtga acggtggggt aatgttatca agatattcgt cggtgtactg
    13501 acgcatacga atggcgatat cttccgcctg ccccagcgca tattcaaaaa aacagagcgc
    13561 catccgcata tcctgcacgc tgacatccat cgtcgagtgg gtgttgatga aggcataaag
    13621 cgggagatat tcattaagct gattgatatc gctacataaa tcgagactgt attcatccca
    13681 gtcaaaaatc acgccgcaaa ttcgagggtt atgttcgata aatttcagca aatcaacgct
    13741 gttttgtggc cagataatct gaaagccttg cgccaccagc gccgactcca gttctttgat
    13801 gggctcatct ttataaaaga cgccatgcgg tcccataatg gcaatgatgt tcatgtgttc
    13861 ctcctggaaa atccttcctt aatcatagcc tgctcaaacc gtggcgataa aaaagggcca
    13921 ccgaagtgac cctttttcag aacttttgcg aattacgcgt aaccgtagct catcaggcgc
    13981 tgataacgac gattttttaa atcttcagtg cttaacacgt cgagatcggc cagatccgcc
    14041 agcagttgcg ctttcaacga tgccgccatc gcttccgggt tacggtgagc gccgcccagc
    14101 ggttccggga tgatggagtc gatcagtttc agctctttca gacgcggagc gatgataccc
    14161 atcgcttcag ccgccagcgg cgctttgtcg gcgctcttcc acagaatgga cgcacaacct
    14221 tccggcgaga taacggaata ggtgctgtat tgcagcatat tcactttatc gcccacgcca
    14281 atcgccagcg caccgccaga accaccttca ccgataaccg tacaaactac cggtacgccg
    14341 aggcgagaca tttcacgcag gttgcgtgca atggcttcag actgaccacg ttcttctgcg
    14401 cccacgcccg gataagcccc cggggtgtcg ataaaggtga tgataggcat cttaaagcgt
    14461 tcagccattt gcatcagacg cagtgctttg cggtaacctt ctggcgctgg cataccaaag
    14521 ttacggcgaa ttttttcttt ggtttcacga cctttttgat gaccaatgat catcaccgga
    14581 cgaccatcga gacgggcgat accaccgacg atagctttat cgtctgcata cgcgcggtcg
    14641 ccagccagtt cgtcaaattc atcaaatgcc aggcgaacgt aatccagggt ataaggacgc
    14701 tgtggatggc gtgccagttg cgcaatctgc catgcaccga gatcggcgaa gattttacgc
    14761 gtcagttcta cgcttttttc acgcagacga tgcacttctt catcgatgtt aatatccagt
    14821 ttctcatcct gacggctaac cgcagtcaga gaatcgattt tcgcttccag ctctgcaatc
    14881 ggctgttcaa aatcaaggaa attcagactc atagtattcc tgtattagtc aaactccagt
    14941 tccacctgct ccgaaccaat gaggccacgg agatcgttta ataaacgatc gctcggagag
    15001 acacgccacg tcgcgccaaa acgcaaccgc gcgcgtgcat ccgccctctg atagtagaga
    15061 tgtactggaa ttgtcccaga gcggtggggt tccagagact gacggagtcg gtttaaaagc
    15121 tggtcatcaa tttgcctgtc cgtcagcgag atagcaagcc cgcgagcata tttttcccgg
    15181 gcttcgtcaa tatccatcac ttcgcgagcg gtcattttaa gcccaccgct gaagtcatca
    15241 aagctgacct gtccgctgac gataagtatg cggtcttttt ccagcaaatg ctggtattta
    15301 tccagggcgt cagtaaacaa catcacttcc agccgcccgg aacggtcatc cagcgtgcag
    15361 ataccgatac gattgccgcg cttggtgacc ataacccgcg cggcaacaac gagccccgca
    15421 gccgtgatga ctttaccacg ttctgtcggg tgcatgtctt tcagccttac gcctccgaca
    15481 taacgctcaa tctcttttaa atactggttg atagggtgtc cggtcaggta caggcctaac
    15541 gtttcacgtt ccccatctaa taccacctgc tccggccacg gttggcagct ggcataggat
    15601 tgttcaattt gttccggctc ttcggccagc acgccgaaca tatcggcctg accgatagct
    15661 tccgctttcg cgtgttgatc tgccgctttt aacgcatcgc ccagcgagtt catcagcgcc
    15721 gcgcgatgtg gcccaagacg gtcaaacgcc ccggacatga tcagtttttc cagcacgcga
    15781 cggttcaact ttttggtgtc ggttcgggcg cagagatcaa acagttcgcg gaagtagccg
    15841 cctttattac gggcttcgat gatcgcctca atcggacctt cacctacccc tttgatcgcg
    15901 ccaataccat acacgatttc gccgtcgtcg ttgacgtgga aatggtaaag accggagttt
    15961 atatctggtg gcaggatttt cagccccatc cgccagcact catccaccag gcccaccacc
    16021 ttctcggtgt tgtccatatc ggcggtcatt accgccgcca taaactctgc cggatagtgc
    16081 gctttcaacc ataatgtttg atatgacacc aaagcatagg ccgcagagtg cgatttgtta
    16141 aatccgtaac cagcgaattt ctccaccagg tcgaagattt tcatcgccag ttcggcgttg
    16201 attccgttct tttctgcacc ttcagcaaat acagaacgtt gcttagccat ctcttccggc
    16261 tttttcttac ccatcgcacg acgcagcata tccgcgccac cgagggtata accagaaagc
    16321 acctgcgcaa tctgcatgac ctgttcctga tacaggataa tgccgtaggt tggctccagt
    16381 accggtttca ggctttcatg ctgccactgc acgtccggat aggagatctc ttcgcgacca
    16441 tgtttacggt cgataaagtt atccaccatc cctgattgca acggaccagg gcggaacagt
    16501 gccaccagtg cgatcatatc ttcgaagcag tcaggttgca gacgcttgat caggtccttc
    16561 atgccgcgcg attcaagctg gaataccgcc gtggtttccg agcgttgcag catgtcgaag
    16621 cttttcttgt catccagcgg gatcgcggcg atatccagcg gcggctcgcc attcttcgcc
    16681 cgccgcttgt tgatcatctc cagcgcccag ttgatgatgg tgagcgtacg caaaccaagg
    16741 aagtcgaact tcaccagtcc ggcgtattca acgtcgcttt tatcaaactg ggtgaccgga
    16801 tgtttgccct cttcatcgca ataaagcggc gcaaaatcgg taattttggt cggcgcgata
    16861 accaccccac cggcgtgctt accggcgtta cgggtgaccc cttccagttt gcgcgccatg
    16921 tcgatcaacg ccttaacttc ttcatccgct tcgtagattt ccggcagctg cggctcggct
    16981 tcaaacgctt tcgccagcgt catccccgga tcgggcggga tcagtttcga gatacgatcg
    17041 acaaagccgt acggatgccc cagcacgcgg cctacgtcgc ggatcaccgc tttcgccgcc
    17101 atcgtaccga aggtgataat ctgcgatacc gcatcacgac cgtacatgtc cgctacgtgt
    17161 tcgataacct gatcgcgttt ctccatacag aagtcaacgt cgaagtcagg catggagaca
    17221 cgttccgggt taaggaaacg ttcgaacagc aggtcaaatt ccagcggatc gaggtcggtg
    17281 attttcagcg cgtaggccac cagtgaaccc gcaccggagc cacggcctgg ccctaccggt
    17341 acgccgttat ctttcgacca ctggataaat tccataacga tgaggaagta gcccgggaag
    17401 cccatctggt tgataacctg aagttcagtc tccagacgtt cgtcatattc cgggcggcgc
    17461 ttaacacgtt cttcctcatc agggaataaa aaggccagac gctcttccag gccctctttt
    17521 gcacgcttga ccagataatc ttcggtgctc atgtccccgg tcgggaactg cggcaggaag
    17581 tattcaccaa gacgcacagt tacgttacag cgtttggcga tctcaacggt gttggcaagg
    17641 gcttctggga tgtcggcaaa cagctcgcac atctcctctt cgctacgcat atattgctgc
    17701 ggcgaatagt tacgcgggcg tttaggatcg tcgagggtaa agccgtcgtg gatcgcgacg
    17761 cggatttcgt gtgcgtcaaa gtcgctgctg tcgataaagc gcacatcgtt agtcgccacg
    17821 acgggcaaac cgcgcgcttc cgccagttcc accgccgcgt gcagatagct ttcttcatcc
    17881 ggcctgccgg tgcggatcag ctcgagaaaa tagcgatccg ggaagtgttc ttcataaaac
    17941 gcgacacact catctaccag cgcgctgtta ccacgcaaaa gactgcgtcc gacgtcgccc
    18001 atgcgcccgc cggaaagaag gatcaaccct tcgtttaatt cgataagcca gtcgcgatcg
    18061 atgatcggcc cggcggcacc gtacccgcgc tgatacgctt ttgagatcag caacgtcaga
    18121 ttctgatagc cggtattgtt cgccgccagt accgtcaggt gggttaactc atcacccagc
    18181 aggtcgcact ggacgttaaa atctgccccg acgataggct taatccctgc gccatgtccc
    18241 gctccgtaga acttcaccag accgcaaagg ttggtgaaat cggtgatcgc cagtgctggc
    18301 atacccaacg ccgccgcctt ttttaccagc ggtgcggttt tggccaggcc atcgatcatc
    18361 gagtagtcgc tgtgcacccg caggtgtacg aaacgtggtt cagacatctt cagattccgg
    18421 tttacttaat ctcgacacaa gaatcaggac gcaagtccca gtgcgcgttt gacaggccca
    18481 aagctgcgcc gatggtgttc ggtcgcgccg tgttcagcca gtttttccag atgaaaagcg
    18541 gttggatacc ctttgtgttg ggcaaaacca tattgcggga aaacaatatc cagcgccgcc
    18601 atttcggcgt cacgcgtcac tttcgccagg atagacgcgg cactgatttc cggtacgcgg
    18661 ctatcgcctt tcaccacagc catcgcaggc atcggtaatt tcgggcagcg gttaccatca
    18721 atcaacacat attccggcgc aatatgcagc ccagcgacgg ctcgctgcat cgccagcatg
    18781 gtcgcatgta aaatattcag ttcgtcgatt tcgtgaggtt ccgcgcggcc cagactccag
    18841 ctcaacgctt tctctttgat ctcttcacag agcgccagac gacgtttttc gctcagcttt
    18901 ttggaatcat tcagcccggc aatcgggcgc gccgggtcga gaatcaccgc agcggtgacg
    18961 accgcgccaa ctaacggccc gcgtccgact tcatccacac ccgcaaccag ctgcgtgtgc
    19021 ggatagacaa attcgatcat tgtgctaact ccagaacggc ttgtgccgcc tgctcatcgg
    19081 cattgcagcg gatctgctga tgcagttcac ggaaggtatc gtgcatcgcg tggctggttt
    19141 tcccattcgc caacagcggt aacagcgccg cagccagttt ttgcggctca cactcttcct
    19201 gcaataactc tttgactaac tctctgcccg ccagcagatt tggcagcgag acataatcag
    19261 ttttcaccag ccgcttcgcc aaccagaagg taaaaggctt catgcgatat cccaccacca
    19321 tcgggcattt cgccagcata cactccaggg ctgccgtccc cgacgccagt agtgccgcat
    19381 cgctggcgac catcgcctca cggcccattc catccagcaa atgaaccgac aggtctggcg
    19441 cgactgcagc tttgatgcgt tcaaactgct cgcggcgttt ggcattcacc agtggcacca
    19501 cgatttcgag atccggatat gtctggcgca aaagctgggc cgttttcagg aaatcggcac
    19561 taagcatttc gacttctgca ccacggctgc ccggcaacaa tgccagacag tgggcatcgt
    19621 aagggatccc cagcacatca cgggcactat ttttatctgg atctaatggc atggcatcag
    19681 ccatggtatg accgataaag cggcacggta cgttgtattt gtcataaaac gctttttcga
    19741 aaggcagaaa tgcgagcacc agatcggtgg ctctgcctat tttgaaaacg cgtttctgtc
    19801 gccacgccca gacggacgga ctgacataat gaatggtttt gataccctgc tttttaaggt
    19861 taccttcgag gataatattg aagtcaggcg catcaatgcc aacaaaaaca tctggcttta
    19921 gttcaccaaa acgctttgtc agatcggcac gaatatgcag taagcgacgc agacgaccga
    19981 gcacttcaac aatgcccatc accgccaact cttccatttc gtaccaggct tcgcagcctt
    20041 cagcctgcat tcgtggcccg gcaacaccaa caaagcgggc gttgggcaca cgttctttca
    20101 gagcgcggat taaaccggcc cccaggatat cgccggaggt ttctccggcg accagggcaa
    20161 tcgttaatgg acgctgttca gtcattaacg aatcagaccg cgcgttgagc gtgcaaagaa
    20221 atcggtaaag gctttcactt ccggatatgt ttccgccagt tcagcaattt ccggtttcac
    20281 ttcatcgagc gttttaccgc tacgataaat cagcttatac gcattgcgga tagcggtaat
    20341 cgcctcacgg ctgaatccgc ggcgcttcag cccttcgata ttgacaccga acggcgtggc
    20401 gtggttaccc tgcgcaatga cataaggagg gacgtcctgc gccacaccgg agcagccgcc
    20461 aaccatcaca tgcgcaccaa tgatgcagaa ctgatggact gcggccatgc cgccgatgat
    20521 cgcgaagtcg tcaaccgata cgtgacccgc cagcgttgcg ttgttggcga gaatacagcg
    20581 gttacctacc gtacaatcgt gcgcaatgtg cgcgttgatc atcagtaagt tgtcgctgcc
    20641 caccttcgtc aatccaccgc cctggactgt gccacgatga atggtgacgc tttcgcgaat
    20701 gcggttacga tcgccgattt ccacgcgggt cggttcgcca gcatatttca gatcctggtt
    20761 aacttcgccg atggaggcga actgataaat ctcattatcg cggccaattt tagtatgacc
    20821 attcacgaca acgtgagatt tcagtacggt accctcacca atttcgacat ggggtccaac
    20881 gatacaaaaa ggaccaatgt gtgcgttcgc gccaatcgac gcgccctctt ccacaatggc
    20941 ggttggatgc acaaaggcgg atttatcaat cacgtatcag gcctcccggc tacgagcaca
    21001 catcatcgtt gcttcgcaaa ctactttacc gtcgaccaga gcaacccctt taaaacgggt
    21061 caggccgcgg cgcgtttttt cgaaagtgac ttccatgatc atttgatcgc caggcacgac
    21121 cgggcgcttg aagcgcgctt cgtcaatacc agcgaagtag tacagctcac ccggttccag
    21181 ttttcctacg cttttaaacg ccagaatacc tgttgcctgt gccattgctt ccagaatcag
    21241 cacacccggg aaaatcggtt ttccagggaa atggccctgg aagaatggct cattgacaga
    21301 gacatttttt actgcgcgca gaaaacgacc ttcttcaaaa tccagcacgc gatccaccag
    21361 taagaacggg aaacggtgcg gcagaagttc taaaatctct tcaatctgca gagtatgagt
    21421 gttagtagtc aagatactct tcctgtcaaa atataagaaa cgacaataat aacacggcct
    21481 gccgcaatcg taagaatgag acaggccgta aagtttggcg aacaaaagat ggaacgttag
    21541 tcttgttgat taaccttgcg ctcaagcgat ttcagacgct tgctcatgtc atcaatgttc
    21601 atcaccagtg cagcggtttt gcgccagact ttgttgggtt gcagcggaat gcctgaggaa
    21661 tagacgcctg gttcagtgat gggacgcatc accataccca tgcccgtaac cgtcactttg
    21721 tcgcatattt ccatatgccc gttgattacg ctggctccgc cgatcatgca gtaacgacca
    21781 attttcaggc tgcccgccat aatgacgcca ccggcaaccg ccgtattgtc gccaatcacg
    21841 acgttatgtg caatctggca ctggttatca atgatcacgc cattgccaat aacagtgtca
    21901 tccagcgcgc cgcgatcgat ggtagtgcag gcaccgatct ccacgcgatc gccaataatt
    21961 acgcgaccaa tctgtgggat cttcacccag ttaccacgat cgttggcata accaaagccg
    22021 tctgcgccta ccactgttcc ggactggatc aggcaattct gaccgatctg gatctcatgg
    22081 taaatggtta cgttcgccca gagacgcgaa cctgcaccga ttttgctgtt tttacctacg
    22141 aagcaaccgg caccgataat cacgttatcg cccagttcaa cgccggattc aatcaccgcg
    22201 ttagcgccaa tcgatacgtt gttacccagc ttcgccgtcg cgtcgatcac cgcactgggt
    22261 gcaatgttct gcgcgggctg cggcgtggta tctaaaattt gcgccatgcg cgcgtaagtc
    22321 aggtagggat tcttcactac cagcgcggca cttttcgcga aaggaagatc gtcctgggtc
    22381 atgacaaccg cggacgcctg gcacaagcct aaatgctcac ggtattttgg gttaaccatg
    22441 aacgtaatgt gacctgtttg tgcagattgc atggacgcaa cgccggtgat gacgatatcg
    22501 ccatcaccgt gtagttctgc atccaactgc tgcgctaaat cagccagtcg aattgaaggc
    22561 attacttatt taacctgttt cagtacgtcg gcagtgatgt cttttacatc gctgctgttg
    22621 taagcaacgg cgtttgcatc aacaaccaga tcgatatcct ggctgttggc aacggatttc
    22681 acagcagtct ggatacgagt aaccagtttg ccgcgttctt cattggaacg acgtgcgcga
    22741 tcctgctcaa aagcctgcgc tttctgagca aaagtctggc gctgagccat cacgtctttt
    22801 tccagcttag tgcgatcgct gcccgctttc atggactgca gctttttcat tttagcctgc
    22861 agatcggttt ccatacgctg cagttcgctg gcacggcctt tgaactcatt ttccagcgtg
    22921 ttagaaacac cggttttctg cgctacctgc tggaacaggc tgcccatgtt gacgattgca
    22981 attttgtcag ccgcctgagc agatgttgcc agtgctaaac cgagacctgc agctaataac
    23041 cactttttca caataaactc cttaccatcc catttgcacc ggagggtgca gttctttgcg
    23101 tggcccggcg atttatattg atcgcctaaa gtcatcgcta cactaccact acattccttt
    23161 gtggagaaca cttaccaggt tttaccgatg ttaaactgga actgttctgc cttgtctcca
    23221 tcgtactttt tgaacggctg ggcgtaggag aacaccaacg gccccaatgg ggacatccat
    23281 tgtaatgcga tacccgcaga catacggata ttgcttggat cactatagtc cggatatcca
    23341 gaatattggc tggaatccca gtttgtatcc caaacggtac ccatatccca gaagaaggaa
    23401 gtacgaaccg agttagcata cttatcgcta ataaacggcg tcggggtgat gaactcgagg
    23461 ctggcaaccg ccatggcgtt accgcctaca gcatcatccg atttacacag gtctttcgcg
    23521 ccgtcctgag tcgcacattc gtaatcatag tccggatcat aattactggc ctgatgcggg
    23581 aagtaaactg ctttcggacc aattgtattg gactggaagc cacgcacggt gctggaacca
    23641 ccggcataga agttctcgta gaacggcatc tctttgccgc ctaaaccatc accataaccc
    23701 cagcgggtac gccccagaac aacccatttg tgatcgtcat cgatcggcac ataagtcgcc
    23761 gtgtctaacg tcactttgta gtattcgtta tccgatccag gaatggtcac tttaccggtc
    23821 aggttgacac gtgaaccatc tgtcgggaag taaccacggt caagcttgtt ataggtccaa
    23881 ccatagttga acgtgaagtc gtccgttttg aagctgttat cctgatcaga ggtgctcgga
    23941 tgttcaccca tagagtacag ataacgccac atcgcaacct gaggctgcat gttggacagg
    24001 gagttatgta cataacccag acctgcacgc agcgagttat attcgttaat cgggaagccc
    24061 aacgtcacgt ctgtaccata actcttgttg gtatagtcgg acaggtcggc gtcatctgcc
    24121 tggaagtcat tatagaagag acgaccaccg aggcttacgc catctacggt gaagtacggg
    24181 ttggttaccg acagttcagc ataggtctgg taatcgtttt tggtcccgtt gataccaaca
    24241 gcataacctg tacctaacca gttatcctgt tgcacgccag cctggaagct cacgccactt
    24301 tcagtaccgt aaccaatccc gaagttgaag ctaccagtgt tacgttcttt caccttgtag
    24361 actacatcta cctggtccgg gctacccgga acacgttggg tatcggtatc gacagtttca
    24421 aagaagccca gacgattcag acgctcctta ccctgatcga ccagatcgct tcccagccat
    24481 gcaccttcca tctgacgcat ttcgcgacgc aggacggcat ctttcgaggt atcgttacct
    24541 tcaaaacgga tcttacgcac gtagaaacgg ttacccgcat caacgttcac gcgtaattta
    24601 acggttttgt cggcatcgtt aatttcaggc atcgactgta cgcgcggata ggcataacca
    24661 tagcgaccga gaagcttttt aatgtcatct tccattttgg tcactttggt gccgttatac
    24721 agctcgcccg gctcgatctt agtcagctgc tcaatttcag cggagtgccc ggcaaggttg
    24781 ccgctcactt caacgccaga aagcttgtac tgatcgcctt cggtgatgtt caccgtgacg
    24841 taaatacctt ttttatctgg cgtcagactg acctgggtag agtctatgtt gaaacgggca
    24901 taaccgcggt ccagatagta gctgcgcagg gtttcaaggt cgcccgccag tttctgtttc
    24961 tggtatttac gatcgcctac cacgttccac cacggcactt cgtcacgcag ttggaaatga
    25021 gagatcagtt cgtcggtggt gaaagcatgg ttaccaacaa tgttaatttg ctggatttca
    25081 gctgacacac cttcctggaa caccagtttt aggtcaacac ggttgcgcgg cagcggggtc
    25141 acgacagctt taacgctggc gctatattta ccgacgctgt agtagaagtc ttccagacct
    25201 ttctcgatat cggcaatggt ggtgcgatcg agggattcgc ccacacgcac accagaagcc
    25261 tcgaggtttt gcttcagcat gtcatctttc accgatttgt taccggagaa agtaatgctg
    25321 gcaatggtcg gacgttcttt tacctgaacc agaagggtat caccatcacg aaggacgcga
    25381 acatcctcaa agttgccggt agcaaacaga gcgcgaatgg tattactgat atcttcatca
    25441 ttaaccgtgt cgcctgtgcg caccggcata ctgaggaggg ccgcaccaac ggcgacacgc
    25501 tgaaggcctt cgaaatgaat atctttcact acgaaccctt cagcaccgta tacggtggcg
    25561 ctgctaaaca gcagcgacgc tatgagcaac tttttcatcg ccatcgttat tatgcgttct
    25621 tcctaactaa ctctcataac cgagagaaat cattgaaaag tgcaagcccc attaacagca
    25681 ccagcagaat cgagccaatg cgataacaaa agtcttgaac ccgctcggat accggtccgc
    25741 ctttgatctt ttcgatcgca aggaacagca gatgcccccc gtcaagtacg ggcaacggaa
    25801 acaggttaat tatccctaag ttcacgctaa taagcgcaag aaacggcagg taataaacta
    25861 ccccgagttc cgctgtcatc ccagccccct tggcgataga gatcggccca ctgaggttgt
    25921 tcagtttcac atcaccggtg atcaattttc ccagcatact gaccgtcagc ttcatcagct
    25981 gccacgtttt gtccgtggct tcgacgatgg cgttgaacgg cccatactgg cgtacaactt
    26041 tatactcatc tggcaaagga atgactttcg gctcaatacc gacaaaacca atcgctttac
    26101 cattacccgg tttactctcc gggattaatg tcaaagacaa gggactcccc tgcctttcga
    26161 tttctaacgc taaggattta cccgggttat cccggacaag catcacaaag gtcacccact
    26221 gcgttaaggg ctgaccatcg actttaacga tcctgtcgcc tgcttgcaaa cctgccttgc
    26281 ttgccgccga gtttggctgc acattttcca gtacaggttc aatttgcggc ccacgaggac
    26341 gaatccccag cgaagatacc ggatcttctt tatcaggctc aaacgcccag tgacgtaaat
    26401 cgagctttac atcccgccgt tggtcgctgc caaatggcgc tactgtaatg gtggtgcttt
    26461 catcgccaat tttatcgacc aactgcaaac gcacggcatc ccaatcaggc gtttcgatac
    26521 tatctacggc ttttagttcc gtacctggtg caatttgtgc ttccgcagct atcgaattgg
    26581 ctgctatttc gccaaccacc ggacgtacgc caggcacacc aataataaaa accagccagt
    26641 aggcaaagat agcaaaaatg aagtttgcaa ccggacctgc ggcaataatc gccgctcgtt
    26701 ggccgacaga tttattattg aaggcatggt ggcggagttc cggaacgacc ggttctgcgc
    26761 gctcatccag cattttgaca taaccgccca acgggatcag ggcgataaca tattcggtgc
    26821 cgagcttatc agttcgccgc cagagcgcct taccaaaccc tattgagaaa cgctcaacgc
    26881 gaacaccaca acgccgggca acccagaaat gaccaaattc atgcacggtg ataagtacac
    26941 ccagtgcaac gatgaacgaa gccaaatccc agagaaaact cagcatatta ccttccgtta
    27001 aagcgtcctg aataccagta acaacaagca agcaaagacc ggtaccgcag ccgtcaggct
    27061 atcaatacga tctaaaatac caccgtgtcc tggaattaaa tgaccgctgt ccttaattcc
    27121 tgcttcacgc ttaaacatac tctcggtcag atcgccgagc actgaggcta acgctgcgac
    27181 aatagagcaa atgagtaagg tgacgggagc gacgtcgaga ttcgcccaca tgccataacc
    27241 ccatgagatt accgctgcag tagcgagtcc accgataaag ccttgccagg ttttacccgg
    27301 agaaaccttc ggtgccagct tatgtttacc aaacaatttg ccaaacatat atgcgccgga
    27361 gtcagcgccc cataccagga tcatgacata gagcagccat attgcgccac tgtaatgatt
    27421 ctcgtcatag tgccaggccc gtaacgccag catgccccag aagaagggaa caatggttag
    27481 cacgccaaaa ataaggcgca atgttttaga gttacgccag attgctgcgg aacctgggta
    27541 aaacagcacc agcaatagcg cgacaatcca ccaacccagc gaagcccaaa gtgagatttc
    27601 aaccagcggt tgatgaatat ttcggtgata ttccggcaac agaaaaagca tcagcgccaa
    27661 caataacccg cataacaccg ccaaccatac tcgctgcgaa cgagtggtaa aaccgctaag
    27721 ctgtccccat tcccacgctg ccagcatgca gaccaccagc gttacaatgg cgaaccccac
    27781 cggcggcaac agaaacaacg ccgcgatgac gacgggtatt aacacaaaag cagatatcag
    27841 gcgatacttc agcaaaagcg acccccatca ggctgtttca tcaccgggct cggtgccgcc
    27901 gaaacgacgc tctcgattag caaaggcatt taacgcccct tcaaagtctt gttcatcgaa
    27961 atcgggccag agaacatctg taaagtaaag ttcggcatag gcaatttgcc aaagcaaaaa
    28021 gttactaatg cgatgctccc ccccagtcct aattactaaa tctacagggg ccagttcatg
    28081 catacagaca tgctggttta gcatctcttc atctatctga tctggttgca ggtttccttg
    28141 ctgcaccttt tcagccagtt gcctgactcc ctggactata tcccaacgtc caccgtagtt
    28201 cgccgcaata ttcagcgtca gaccggtatt cccggctgtt agcgcttcag atttacgaat
    28261 acgttcttgc aaacgcgagt taaagcgact ggtatcgcca ataatacgca gacgcacgtt
    28321 atgtcggtgc agacttttta cttcgctatc gagcgcccac acaaacagtt ccattaacgc
    28381 actgacttcc tgcgctggtc ggttccagtt ttcactacta aaggcataca gcgttaacgc
    28441 ctcaataccg ttgttggccg caaaagagac agcccggcgg acggattttg ctccggcttt
    28501 atgcccaaag gcacgaatct tcccttgctt ttttgcccag cgaccattgc cgtccataat
    28561 gatcgcaaca tgacggcagc catgcgctgg caatttttcg ctaagtggtt gagtagcaga
    28621 caacatcacg cgttttttat tccctgacag ggtttacggt actcaggaat actgaagcct
    28681 atacaaaaaa aagccgtgta aaaccacggc atacctgacc ttataaagcc aactacttac
    28741 gatcagatgg cgcagactat atcactgaag ccctacgcta acaaatagcg cgactctctg
    28801 tagccggatt atcctcagct tgcgagacgc atcacctctt ttctggcgac ttcacgcgcg
    28861 ctcgcatcaa cagataacac atcgtccaca cattgtggtt cgcgcatatc cattttttcc
    28921 agtacggata aattcaacgc agcgatatcc gtaaagcgga tttgttgcgc aagaaaagca
    28981 gcaacggtga tttcgtttgc ggcattcaat gctgtcgtcg ctgcctggcc ttgttcgaac
    29041 gcctccatcg ccagtttcag gcatggataa cgatcataat ccggtgcggc aaatgtcaac
    29101 gcacttagtt tgcaaaaatc gagcggcttc acgccagagt tcacgcgatt cggccatgcc
    29161 atggtgtggg caattggcgt acgcatatcc ggttccccca gctgcgccag aacactgccg
    29221 tcctgatagc gcaccattga gtgaatcact gactgcgggt gaatcagcac ttccatctgg
    29281 ctggcgctgg cgttaaacag ccaacgcgct tcaatgtatt ccagaccttt gttcatcatg
    29341 gtagccgaat cgacagaaat tttacgtccc atcgaccagt tcggatgacg gcaggcttga
    29401 tcgggcgtca ttgttgccaa atcgcgcaat ggcgtctcac ggaaagggcc accagacccg
    29461 gtaagtaaaa tggacaccac gccattttgc tcaaggtcag cgtatcccag attatgctgg
    29521 ataggttgcg gtaaactctg aaaaatggcg ttatgttcgc tatcgaccgg taacaattgc
    29581 gctttgctct gctttacggc gtccataaac agacgtccgc aggtaaccag tgattcttta
    29641 ttggccagca aaatggtttt acccgcgcgg atcgcagcaa gcgtaggtaa cagcccagca
    29701 gcgccaacaa tggctgccat cacctgatca acatcctcaa gcgctgccat atcgcaagcg
    29761 gcttgttgcc cacttaagac ttcggtgcgg ctaccctgtt gctgtagcat cgttttaaga
    29821 agtttcgcac tcgcttcatc gtccattacg gcatagcggg gagagaattc caggcactgt
    29881 tctaccatgc gagtgacatt tttgcctgcc accagcgcaa ctacgcggaa gtgttcggga
    29941 ttatggcgca ccacgtccag cgtgctgcaa ccaatcgagc cggtcgagcc cagaatggtg
    30001 agttgcttca tgaaacatcc agagttgaga cagaataaaa agcaaaacgc cgccagccga
    30061 tccgcaagga tctactgagc ggcgtttttg tcgttcaaga aatcagaact gcatcagttc
    30121 tgcttctttg tctgccagcg ccgcttcaat tttcttgatt gcagcatcag tcagtttctg
    30181 tacatcgtcc tgagaacggc gatcgtcgtc ttcgctgatc tctttatctt tcaacagtgc
    30241 tttcactttg tcgttcgcgt cacgacgcac gttacgtact gcaacacgcg cttgttctgc
    30301 ttcaccacga acgattttgg tcagatcttt acgacgttct tccgtcagcg gcggcagcgg
    30361 aacacggatg tcgctacccg cagagttcgg gttcaggcca agatcggacg ccataatcgc
    30421 tttttcaacg gccggagaca ttgaacgatc aaacacgttg attttcagag tacgggaatc
    30481 ttctaccgtt acgcttgcca gctgacgcag cggcgtcggc gtgccgtaat attccacgac
    30541 aatgccatcc agcaggctgg gagaagcacg acccgtgcgt attttgctga tttgggtttt
    30601 gaacgcttct acgcatttgt ccatgcgtac ttcagcatct tttctgatat cgctaatcac
    30661 gttacgaatc cttgaaaact tgtctcagtc agaccagaca gtcacacaca gtggaagtgt
    30721 gctaagtata gccctgatta aacatattac gggtaagatc ccccgcgata cttacgcgga
    30781 atcttaccct tatttatcca tcacgggaat tattccgtga ttaaagtccc ttctttttca
    30841 cccattacca cacggcgcag cgcacccggt ttgttcatgt tgaaaacacg aatcggtaat
    30901 ttatggtcac gagccagcgt gaaggccgcc aggtccatga ctttcagctc tttttccagc
    30961 acttcgctgt aagtcagttg ctcgtacatg gttgcggttg gatctttcgc cggatcagcg
    31021 gtaaacacgc cgtcaacttt ggttgctttc agcaccacat cggcttcaat ttcgatacca
    31081 cgcaggcaag ctgctgagtc ggtggtaaag aacgggttac ctgtaccggc ggagaggatc
    31141 accacacggt tgttgcgcaa caggctgata gcttctgccc agctgtagct gtcgcacacg
    31201 ccattcaatg gaatagcgga catcagacga gcgttcacat aggcgcggtg cagtgcatca
    31261 cgcattgcca ggccgttcat tacggtcgcc agcatcccca tgtggtcgcc cacaacgcgg
    31321 ttcatacccg ctttcgccag accagcgcca cggaacaggt tacccccacc aatcaccaca
    31381 ccaacctgaa tacccagttc aaccagttct ttgatttcct gagccatacg atccagtatg
    31441 cttgcatcaa taccgaagcc ttcagtgccc tgcagagctt cgccactcaa cttaagcaga
    31501 atgcgtttat agacgggttt tgcattggta gccatgtttc tttcctgaga ctgtcaacga
    31561 ttgaaatgaa ttaatactag cgacattgta cgtcgctttt cagcgcagcc ccacaagggt
    31621 tagctgaatt tacaagatgg gcacaaaaag aagccgccct caggcggctc ctttttgata
    31681 attaagactg cttggacatc gcagcaactt ctgctgcaaa gtcagtctca actttctcga
    31741 tgccttcacc cacttcgaag cggatgaagc cagtcacttc agcgttatgc tctttcagca
    31801 gctgaccaac agttttgctt ggttccataa cgaacggctg accggtcaga gaaacttcgc
    31861 cggtgaattt cttcatgcgg ccttcaacca ttttctctgc gatttctttc ggcttaccag
    31921 actgcatcgc gatatccagc tgtacctggt attctttttc taccacttca gcggatacgt
    31981 cttccggttt gatgaattct ggcttgcttg cagcaacgtg catagcgatg tgtttaacca
    32041 gctcttcgtc agcgccttta gcagcaacca gaacgccgat acgcgcacca tgctgataag
    32101 aacccagaac gtcgccttcc agcgcagcaa cgcggcgaat gttgatgttt tcaccaattt
    32161 tcgctaccag cgcaacacgt tcttcttcga actgtgcttt cagaacttca acgtcagtga
    32221 ttttgccagc aacagctgcg tccagaactt tgtctgcgaa cgcctggaaa ccagcgtctt
    32281 ttgcaacgaa gtcagtctgg cagttaactt ccagaatgat gccgtagttg ccgtcgattt
    32341 tggttttgat cacgccgtca gcagcaacgt tgcctgcttt tttcgctgct ttaatagcac
    32401 cggacttacg catgttttcg attgccagct cgatgtcgcc gttagcttca gtcagtgctt
    32461 ttttgcaatc catcatgcct gcgccagtac gctcacgcag ctcttttacc agggatgcgg
    32521 taatttcagc cattctaaaa tcctcggaga tgtgatctgc ccggcattaa accgcacaga
    32581 tataaaagtg aaaaaggggg ccatatgcag gccccctaac caaacgtgta ctacctggtc
    32641 tataagggct ctcccccttc atatttcgcg ccgcagatgc gtcatcttcg ctcgctcatc
    32701 ccggtcactt actgatgtaa gctcccggga attctcgagc ttgccgcctt tctgcaactc
    32761 gaactatttt gggggagtta tcaagcctta ttactcagct tctacgaagc tttcttccgc
    32821 ctgggaagcc agatcctgag aacggccttc acgtacggtt gcagcaacag cgcccaggta
    32881 cagggtcaca gcacggattg cgtcgtcgtt acccgggata acgaagtcaa caccgtccgg
    32941 atcagagttg gtatcaacga tagcaaatac cggaataccc aggttgtttg cttctttgat
    33001 agcaatgtgt tcgtggtcag catcgattac aaacagagcg tccggcagac cgcccatgtc
    33061 tttgataccg cccaggctgt tttccagttt ctccagctca cgagtgcgca tcagcgcttc
    33121 tttcttggtc agcttgtcga aagtaccgtc ctgagactga gtttccaggt ctttcagacg
    33181 tttgatggac tgacgaacgg ttttccagtt agtcagcata ccgcccagcc agcgatggtt
    33241 cacgaagaac tggtcgcagc tcagagcagc gtctttcacc gcttcgcttg cagcgcgttt
    33301 agtaccaacg aaaaggattt tacctttgcg agaagcaatc ttgttcagtt cagccagagc
    33361 ttcgttgaac atcggtacag ttttctcaag gttgatgatg tgaactttgt tacgcgcacc
    33421 gaagatgaac ggcttcattt tcgggttcca gtaacgggtc tggtgaccga agtgaacacc
    33481 agccttgagc atgtcgcgca tggaaacagt tgccatgatt aaaacctcta tataaaagtt
    33541 ggggttatgc ctccacgtat cccatattac cgaccccaaa gggcaccccg gaatatgtgc
    33601 cgatacgtgt gtgttgttac acaaagtgag attgtcgctt ccgtccagtc tgtgtatacg
    33661 aaatggatcg gaagtccggc gcgctttata ccacaaatac gccgtggaca ccaataattg
    33721 ttggcgctgt gtacagcatc agacgtcgaa ttttctatta tagaaaacct tcagtggcac
    33781 gtttggcgaa attcagaatg attctcaatt tgcccgggtg tgataccatt gacggcactt
    33841 acatatatat tgtcggtatc accgacgctg atggacagaa ttaatggcta tctcaatcaa
    33901 gaccccagaa gatatcgaaa aaatgcgcgt cgctggccga ctggctgccg aagtgctgga
    33961 gatgatcgaa ccatatgtta aaccgggcgt cagcaccggc gagctggatc gcatctgtaa
    34021 tgattacatt gttaatgaac aacacgcggt ttcagcctgc ctcggctatc acggttatcc
    34081 gaaatccgtt tgcatttcta ttaatgaagt ggtgtgccac ggtattccgg acgatgctaa
    34141 gctgctgaaa gatggcgata tcgttaacat tgatgtcacc gtaatcaaag atggtttcca
    34201 cggcgatacc tcgaaaatgt ttatcgtcgg taagccgacc atcatgggcg aacgtctgtg
    34261 ccgtatcacg caagaaagcc tgtacctggc gctacgcatg gtaaaaccag gcattaatct
    34321 gcgcgaaatc ggtgcggcga ttcagaaatt tgtcgaagca gaaggcttct ccgtcgttcg
    34381 tgaatattgc ggacacggta ttggtcgcgg cttccatgaa gaaccgcagg tgctgcacta
    34441 tgactcccgt gaaaccaacg tcgtactgaa acctgggatg acgttcacca tcgagccaat
    34501 ggtcaacgcg ggtaaaaaag agatccgcac catgaaagat ggctggacgg taaaaaccaa
    34561 agatcgcagc ttgtctgcac aatatgagca tactattgtg gtgactgata acggctgcga
    34621 aattctgacg ctacgcaagg atgacaccat cccggcgata atctcgcacg acgaataaaa
    34681 tgaagccggc gaatgccggc ttttttaatg cgataattta atcttatggg tggcgcacaa
    34741 tgaataccct tccagaacag tacgcaaaca ccgctctccc caccctgccc ggtcaaccgc
    34801 aaaatccatg cgtctggccc cgtgatgaat taaccgtcgg tgggataaaa gcccatatcg
    34861 atactttcca gcgttggctg ggtgatgcct ttgacaatgg gatctctgca gaacagttga
    34921 ttgaggcgcg caccgagttt atcgaccagc tcctgcaacg attatggatt gaagcgggat
    34981 tcagccagat tgccgacctg gcattggtcg ccgtcggtgg ctacggtcgt ggcgagctgc
    35041 atccactttc tgacatcgat ttactgattt taagccgtaa aaagctcccg gacgatcagg
    35101 cgcaaaaagt gggcgagctg ttaacgctgc tctgggatgt aaagctggaa gtcggtcata
    35161 gtgtgcgcac gcttgaagag tgcatgctgg aagggttatc ggatttaacc gtcgccacca
    35221 atttgatcga atcccgctta ttaattggcg atgttgcgct atttcttgaa ctgcaaaaac
    35281 atattttcag cgaaggattc tggccttccg acaagttcta cgcggcgaaa gttgaagaac
    35341 agaaccagcg ccatcagcgt taccacggca ccagctacaa tcttgaacca gacatcaaaa
    35401 gtagccctgg cggcttgcgc gatatccaca ctctgcaatg ggtggcccgc cgtcattttg
    35461 gcgcaacatc gctggatgaa atggttgggt ttggcttctt aacctcagcg gagcgggcgg
    35521 aattaaacga atgtctgcat atattgtggc gtattcgctt tgccctgcat ctggtcgtca
    35581 gccgttacga taatcgcctg ttattcgatc gccagcttag cgtcgcccag cgtctgaatt
    35641 acagtggcga aggtaacgaa ccggtcgagc ggatgatgaa ggattacttc cgcgtgacac
    35701 gccgcgtcag tgaactcaac cagatgctgc tgcaactgtt cgatgaagcc atcctcgccc
    35761 ttcccgccga cgaaaaacca cgtccaatcg acgatgagtt tcagctacgc ggtacgctaa
    35821 tcgacctgcg tgatgaaaca ctatttatgc gccagccgga agccatcttg cgtatgttct
    35881 acaccatggt gcgcaacagt gcgatcaccg gcatttactc caccacactg cgccagttac
    35941 gccatgcccg tcgccatctg caacaaccgc tgtgtaatat tccgcaagca cgaaaactgt
    36001 ttttgagcat tctacgtcac cccggagcgg tgcggcgcgg gctattgcca atgcatcgcc
    36061 atagcgtact cggcgcgtat atgccgcaat ggtcacatat cgtcgggcag atgcagtttg
    36121 atctgttcca cgcctacacg gtggatgaac atactatccg cgtgatgctg aaactggaga
    36181 gttttgccag tgaagaaacg cgccagcgcc atccgttgtg tgtgaacgtc tggccgcgcc
    36241 taccgtcaac tgagctgatt ttcatcgccg cgctgtttca cgatatcgcc aaaggacgcg
    36301 gcggcgacca ctctattctc ggtgctcagg atgtagtgca ttttgccgaa ctccacgggc
    36361 tgaactcacg cgaaacacag ctggtcgcct ggctggttcg ccagcacttg ctgatgtcgg
    36421 tgacagctca gcgtcgcgat attcaggacc cggaagtcat caagcagttt gccgaagaag
    36481 ttcaaacgga aaatcgtctg cgctatctgg tatgcctgac agtggctgac atttgcgcca
    36541 ccaacgaaac gctgtggaat agctggaagc aaagtctgtt gcgcgaactc tatttcgcca
    36601 ccgaaaagca gctacgacgc ggaatgcaaa acacgccgga tatgcgcgaa cgggttcgcc
    36661 atcaccaact ccaggcgctg gcactactgc gcatggataa catcgacgaa gaggtgctgc
    36721 accaaatttg gtcacgctgt cgcgcgaact attttgtccg tcatagccca aatcaactgg
    36781 cctggcatgc gcgccattta ttacagcatg atttaagcaa accgctggta ttgcttagcc
    36841 cgcaggctac gcgtggaggc accgagattt ttatctggag cccggaccgc ccttacttgt
    36901 ttgctgctgt ttgtgccgag ttagatagac gcaacttaag cgttcacgac gcacaaattt
    36961 tcaccactcg cgacggtatg gcgatggata cctttatcgt gctggaaccc gatggtagcc
    37021 cgctatccgc agatcgtcat gaggttattc ggtttggtct ggagcaagta ctgacacaaa
    37081 gtagctggca gccaccacag ccgcgtcgtc aaccggctaa actacgtcac tttactgttg
    37141 aaaccgaagt gacgtttttg ccaacccata ccgaccgcaa atcgttcctt gagcttatcg
    37201 cgctcgacca gccaggactg ctggcgagag tcgggaaaat ttttgccgat ctgggaattt
    37261 cgcttcatgg tgcccgaatt acaaccattg gcgagcgagt agaagattta ttcataattg
    37321 ccaccgctga ccggcgtgca cttaataacg agttgcagca ggaagtgcat cagcggttga
    37381 cagaggccct caatccaaac gataaagggt gatgtgttta ctgatatgaa aagagtttaa
    37441 caatgcagca gttacagaac attattgaaa ccgcttttga acgccgtgcc gagatcacgc
    37501 cagccaatgc agacaccgtt acccgcgaag cggtaaatca ggtgatcgcc ctgctggatt
    37561 ccggcgcact gcgtgtagcg gaaaaaattg acggtcagtg ggtgacgcat cagtggttga
    37621 aaaaagcggt gctgctctct ttccgtatta atgataatca ggtgatcgaa ggggcagaaa
    37681 gccgctactt cgacaaagtg ccgatgaaat tcgccgacta cgacgaagca cgtttccaga
    37741 aagaaggctt ccgcgttgta ccacctgcgg cggtacgtca gggtgcgttt attgcccgta
    37801 acaccgtgct gatgccgtct tacgtcaaca tcggcgcata tgttgatgaa ggcaccatgg
    37861 tcgatacctg ggcgaccgtc ggttcttgtg cgcagattgg taaaaacgtc cacctttccg
    37921 gtggcgtggg catcggcggt gtgctggaac cgctgcaggc taacccaacc atcattgaag
    37981 ataattgctt catcggcgcg cgctctgaag tagttgaagg ggtgattgtc gaagaaggtt
    38041 ccgtcatttc catgggcgta tacattggtc agagcacccg tatttacgac cgtgaaaccg
    38101 gcgacatcca ctacggtcgc gttccggcgg ggtctgtggt tgtttcaggt aatctgccgt
    38161 cgaaagatgg caaatacagc ctctactgtg cggttatcgt taagaaagtt gacgcgaaaa
    38221 ctcgcggcaa agtcggcatt aacgaactgc tgcgtaccat cgactaaaag tatgcacacg
    38281 ggcagcacga cgctgcccga ttgctttctg gttcagaata attcgtccta ctcattcttc
    38341 ctcccccctt ttttaaatac tttttcatct tgtttcggac tgtcatgtaa ttcgcaaaac
    38401 gaagataaat gtgctaatcc atcccatgga atatcgccac aataaagcag gtaatttttt
    38461 gatttttacg ccatgataga agtctggaag aaaaaggctt tctgcgatca cagtcgagcc
    38521 gggcgggcca ggccaaatgc taaaataagc acattttact gactcatttt gaggtaacgc
    38581 tatgtacgac aatctgaaaa gtctgggtat taccaatcct gaagaaattg atcgttacag
    38641 cctccggcag gaagccaaca acgatattct gaaaatctat ttccagaaag acaaaggcga
    38701 gtttttcgcc aagagcgtta agtttaaata tccgcgtcag cgtaaaacgg tcgtcgctga
    38761 tggtgtgggt cagggttata aagaagtcca ggaaatcagc ccgaatctac ggtatatcat
    38821 tgatgagctt gatcaaatct gccagcgtga ccgcagcgaa gttgatctta agcgtaagat
    38881 cctcgacgac ttacgtcacc tggagtcagt cgtaaccaat aagatcagcg agattgaagc
    38941 cgatctggaa aaactaacgc gtaaataatc ccgaattgac ggttaactcg ggcattcccc
    39001 tctccccttc gggctgaggg gaatgagcca gcgccgcctc aaccttaacg cataacctac
    39061 cgctcttcat ccagttgtaa cgccacatac agcagcaacc tgtcatcaaa attgcccaaa
    39121 tcaagcccgg tcagttccga tatacgatta agccgatact ccagggtatt acgatgaata
    39181 aacaacgcct ttgacgttgc cagcggttgc acattgtgac gaaaccacgc cgccagcgtt
    39241 cgtcgcagca agccgttatt gtccatcgct ttcagccgcg ccagcggtcg cgccagttcg
    39301 ttggcctgcc agtcgccacg caaactgtcg agtaacacag gtaacatcag atcctgataa
    39361 aaatagcagc gactttctgg catccgctgt ttacccacca ccatcgtcgt tttcgccgta
    39421 cgataggatc gggcaatact gccaggaccg gtaaaatagt tgcccagtga aacgcgaaaa
    39481 cgcagctggc cgtactcttt catgcgggta atcagttgtt caactcgctt acgatgatct
    39541 tctacatccc agcgcccaaa agagttcaac gccggtttca acaccaccat ttcggttagc
    39601 gagacaatcg ccaccagatt attacgctcg ggcgtagtca gcgcgttttg cagttgttgt
    39661 aactccgcca ttgcgctgtc cacaccaagc tgaccgctgt cgacctcaac aatagccacc
    39721 actcgcggtt gattgagatc gatccccagc cgttgcgccc attcagtaag tgcgggagta
    39781 ttctcctctg cctgaatcag gttcatcacc agttcctccc gcaaacggct atcctgcgcc
    39841 aacaagtgca tcaaccgcga ctgttccagc atcatttcag ccgtcatgca gaccagttcg
    39901 ccatatttac gcagattctc tggttcacct gtcaggccaa ttacgccgac aatttcacct
    39961 tccagccgta acggtagatt aatcccttgc cgcacaccgt gcagatgacg tgccaccgcg
    40021 tcatcgatat cgacgactcg tccctgtgaa agtaccagca atgcaccttc gtgcaattca
    40081 ccaatacgct cacgatcgcc gctgccgata attcgcccac gggcatccat tacgttgata
    40141 ttggtatcga tgatgcgcat ggtacgtgcc acgatatcct gcgccatttt ggtatcaaga
    40201 tgccagccag ccataagtcc tccgttatgc acggcttagc ataaggaagt acgtaacgta
    40261 cggcattgtg caaatgccta aaggatgatg aagatgtatg gagttgtggt gaagttcaca
    40321 gattgtaagg agaccccctt cccgttttca ggaaggggtt gagggagatt actgcattaa
    40381 caggtagatg gtgctgtcgc cgcgctgaat gttcagtgcc agcacagacg gtttgctgtc
    40441 gagaacttta cgcagttcag cgatgttttt cacagcctgc tggttcgcgc caataatcac
    40501 atcacctttc ttcaggccga tctgcgcagc cggagtgccc gttttcacgt tgttcactac
    40561 cacgccctga tctttgcctt tgttgctcat ctcagcgcct tcaatgccgt tgaagatgga
    40621 gctggaatca acctgattct ggctgctctg ctgcagttcc agattcacgt tgacctgctt
    40681 accgtcgcgc agcaagccca gggtcagttt gctgcccacc ggcatagtac ccacttgagc
    40741 acgcagtgcg gcaaagctgc tgatcggctt accgttcagt gaggtgatca catcacccgc
    40801 tttaatgccc gcttttgcag cggaggaatt aggcagaacc tggcttacga aagcaccgcg
    40861 ctgggcgtca actttcatcg ctttcgccag ttcggagttc agctcagtcc ccataatacc
    40921 cagctcaccg cgtttcacct ggccgtattc caccatctgc gaggtcaggt ttttcaccat
    40981 gttactcggg atagcaaaac cgataccgat gttaccgccg tccggtgcta ggatcgcggt
    41041 gttgataccg atcagttcgc cgttcaggtt aaccagcgca ccaccggagt taccacggtt
    41101 gatcgctgca tcggtctgga tgaagttttc gtagttttcg gcattcaggc cgctacgccc
    41161 cagcgcagag acaatcccgg aagttaccgt ctcgcccaga ccaaacgggt taccaatcgc
    41221 tacggtgtaa tcacccacgc gcagtgcatc agaatccgcc atcttaattg cggtcaggtt
    41281 tttcggattc tggatttgga tcagcgcgat atcagagcgc ggatctttgc caaccatctt
    41341 cgcgtcgaac ttacggccat cgctcagttg aactttaatg accgtcgcgt tatcaacaac
    41401 gtggttgttg gtgacgacat agcctttatc ggcatcaatg atgacgccgg aacccagcgc
    41461 catgaatttc tgttgctggc cgccaccatt accgccctgg ccaccctggc agaacggaga
    41521 gctctggaac ggagaacctt cctggcagaa cggagaatca tcaccgaaga actgctggaa
    41581 attacgcggc atacgcggcg tattaacggt tgtgctacct tctacgttaa tgctgaccac
    41641 tgaaggcatc accttttcga gcatcggtgc aaggcttggc atctgctggg ctgtcgttgc
    41701 tgaagaagtc tcagccgccg ttgcagagag cggagataac gccaaaccta aactcagagc
    41761 cagtgcactc agtgctaatg tggttttttt catgtatttc agtctcgatt aacagataac
    41821 gcaaaattgc tgtgttcttc agattcgttt tatagcgcga agttccgaac taaagtttct
    41881 gcaaaaggta aaaatttatt gttcgtcttt acaaaagcct ggttattgtt ctacggccat
    41941 cagacgtcgg tattcatccc aggcatagag gtcggtcata ccgctgatat aatcctgcag
    42001 caggcggcaa cggtaataat attcccatag cggaaactca ggagaatctg acggtaattt
    42061 actgacagcc tcgacatagg ccagccgatg gcgcgtcgag agtttgtgga ataagcgaga
    42121 ttcaataggg gaacgtttca cccgttcttt ttctaccagt tcagtaaagt ctgataatga
    42181 caggcttaat aaagggcgat aaatctctaa taatccgcta atgacccgat agccctgcaa
    42241 ttcaagctgc tcgacatctg gatggctaaa cacatgtttt acagcgacat ttttatatag
    42301 cttaagaaga tcgctgcatt cgctggcatc ttccaataat gcatgattaa acgttccggc
    42361 gaaaatcgca ggcagattat caataaatcg ttgtgccgcg tagggtacca gtttatttag
    42421 ggtgtttacc cgtaaataca taaaaaactg atcttccgta ctgcggctta aactatttga
    42481 gcgtgatttt tcccaggcat tttcaaccac cagcgaaaag agcgaacctt tctcatgctg
    42541 gccccacgcc tcgtgcaaat gatgataaag ctgctcaacg gaaaatattc ttttctctac
    42601 cgcatcttca agatctgcca cacaatagga gatgtcgtcg gcagcttcca taatccacgt
    42661 taatggaaaa cgactgtaaa gcgccaaatt aagttcttta cgcaaccggg caatataggc
    42721 ttcttcagaa agataataac ccggcttttt cattaaatag tgatgtgtct caggcgtttc
    42781 gccacgccac cacgctggac gggtatattt taaaataccg ccaacctgcg cccaggtgag
    42841 attcatccgc atcaatgtat gcaccagacg aatgccttgt gcattcccct caaaatgaca
    42901 taagtcctga cgaatcttgc gccgcagctc gttaagcggt tcttccccgt cccgtaaacg
    42961 tagtaccgcc acgctgcagc gatcgtcagt cagaggctgg ctttcggcat cttccgggtg
    43021 caaacgctgg cgaaaccagt catttatcgc cgcttcgcca aaatgaccaa acggcggatt
    43081 gccgatatcg tgcatcaggc atgacatctc aacaatgctt tcaaaaggtc cggtcagttc
    43141 atccaggccg tatgcttcca gcaatttaag ctctttcaga cggcttaaaa tttctttggc
    43201 gatgtagcgc cccacctgct ggacttccat cgagtgggta agacgcgtgc gcacggcggc
    43261 attgcgctcc agtggaaaaa cctgggtctt ttgttgcaga cgacgaattg ccggagagtt
    43321 gatgatacgc ccgcgatcgc tctcgaagat ccgcaggatc tcatgttcgg ttttaacgcc
    43381 ctgcggtgaa cggtaacgac gatgccagtt tatttttttt cggaaatcaa tctgtgccat
    43441 gtcctctccc gcgtgagaaa tacgcttccc cgtaagcgca tggtaaacta tgccttcaaa
    43501 tcgggcttat cgcgagtaaa tctatgaaaa tcggcatcat tggtgcaatg gaagaagaag
    43561 ttacgctgct gcgtgacaaa atcgaaaacc gtcaaactat cagtctgggc ggttgcgaaa
    43621 tctataccgg ccaactgaat ggaaccgagg ttgcgcttct gaaatcgggc atcggtaaag
    43681 tcgctgcggc gctgggtgcc actctgctgt tggaacactg caagccagat gtgattatta
    43741 acaccggttc tgccggtggc ctggcaccaa cgctgaaagt gggcgatatc gttgtctcgg
    43801 acgaagcgcg ttatcacgac gcggatgtca cggcatttgg ttatgaatac ggtcagttac
    43861 caggctgtcc ggcaggcttt aaagctgacg ataaactgat cgctgccgct gaggcctgca
    43921 ttgccgaact gaatcttaac gctgtacgtg gcctgattgt tagcggcgac gctttcatca
    43981 acggttctgt tggtctggcg aaaatccgcc acaacttccc acaggccatt gctgtagaga
    44041 tggaagcgac ggcaatcgcc catgtctgcc acaatttcaa cgtcccgttt gttgtcgtac
    44101 gcgccatctc cgacgtggcc gatcaacagt ctcatcttag cttcgatgag ttcctggctg
    44161 ttgccgctaa acagtccagc ctgatggttg agtcactagt gcagaaactg gcacatggct
    44221 aagtcactgt tcagggcgct ggtcgccctg tcttttcttg cgccactgtg gctcaacgcc
    44281 gcgccgcgcg tcatcacgct ttctcccgcc aacactgaac ttgcctttgc cgccgggatc
    44341 acgccggttg gggtcagcag ctattccgac tatcctccac aagcgcaaaa gattgagcag
    44401 gtttccacct ggcaggggat gaatctggaa cgcattgtcg cgctgaaacc cgatctggtg
    44461 attgcctggc gtggaggtaa tgccgagcgg caggttgacc agctggcttc gctgggaata
    44521 aaagtgatgt gggtcgatgc gacaaacatt gaacaaattg ccaatgcgtt acgtcaactg
    44581 gctccctgga gtccgcaacc ggataaggcc gaacaagccg cgcaatccct gctggatcaa
    44641 tacgcgcaat tgaaagcgca atatgctgat aaacctaaaa aacgtgtttt tctgcaattc
    44701 ggcattaatc cgccatttac cagtggaaaa gagtcgattc aaaaccaggt actcgaagtt
    44761 tgtggcggag aaaacatctt taaagacagc cgggttccct ggccgcaagt tagccgcgaa
    44821 caggtgttag cacgctcgcc acaggcgatt gtcattacag gcggaccgga ccaaattcct
    44881 aaaatcaaac aatactgggg tgaacagctc aaaattcccg ttattcctct cacgagtgac
    44941 tggtttgaac gtgcaagccc acgtattatc ctcgctgcac aacagctctg taatgcgctt
    45001 tcacaggtag attagcgcct gaccttttta ggtgctcagt ggggattgaa caatgctcgt
    45061 ctattggctg gatatagtcg gcacagcggt atttgccatc tccggcgttt tgttagccgg
    45121 aaaattgcgt atggaccctt ttggtgttct ggtactgggc gtggttaccg ctgtaggcgg
    45181 cgggacaatt cgcgacatgg cgctggatca cggcccggta ttttgggtga aagatcccac
    45241 cgatctggtc gttgcaatgg taaccagcat gctgaccatc gtgctggtgc gccagccaag
    45301 acacttacca aaatggatgt tgccggtgct ggacgccgtt ggtctggcgg tgtttgtcgg
    45361 cattggcgtg aataaagcct ttaatgcgga agccggtccg ttaatcgcgg tttgtatggg
    45421 cgtcattact ggcgttggcg gcgggatcat tcgtgatgtt ctggcccgcg aaatccccat
    45481 gattttacgt acagaaatct acgcaactgc ctgtattatc ggcggtattg tccacgctac
    45541 ggcttattac acattttccg taccactgga aacagccagt atgatgggca tggtcgtgac
    45601 gctattgatt cggctggcgg ctattcgttg gcatcttaag ctaccgacgt ttgcgctgga
    45661 tgagaatggg cgttgaagag agaagatgtg ccggatagtt tatccggcac atgaacaaca
    45721 gattagatac taaaggaaga accgcaaccg caggtgcttt tcgcgttcgg gttggtcacg
    45781 atgaaacgag aaccttccag tccttcggta tagtcaacgg aaccgccgac cagatattgc
    45841 aggctcatcg gatcaaccac caggccaacg ccctgttttt cgatggtcat atcgccttcg
    45901 ttcacctgat catcaaaggt gaaaccatac tggaagccgc tgcaaccgcc accggtgata
    45961 tacacgcgta atttcagatt cgggttatct tcgtcagcga tcaggctttt aactttgttg
    46021 gctgctgcgt cggtaaactc cagcggcagt gctacgtcat cactcatatt ttgctccaaa
    46081 cgacatcggc aattgggcaa attctaaccc aataataatc gccattatct aataccctgg
    46141 tatttcgttc aagtattctc gctggctgat gcggccttgc ttcgcgccag ttgctcagct
    46201 tcctgttttg ccagcgtgcg cgcaagaatc gccgagtata gcggtttccc gccggtaaat
    46261 tgcgctaata gtgttgcgcc aagaccggta ataatcattg gcaaaatgag ctggtagtta
    46321 tcggtcatct ccagaaccag aatgatcccc gttaacggcg cgcgaataga tgccgccagt
    46381 aacgccccca ttccggcaat agcaaacgtc cccgcctcaa ggtgatattg cggaaacagc
    46441 tcaacggcaa ccattccgaa agcggttccc agcacagtac ccagcgccag catcggggca
    46501 aaaataccgc ccggcgcgcc ggaagagaag cagagtaagg tggtaatgac ccgcgcgacg
    46561 aagataaaca ccagcattcc catgctgaaa ttccccgcgg tagcgatagg aatcaggtta
    46621 aaaccgccgc ccgacgttgc tggtgccaca aaccccagca atccacacag accgccaatc
    46681 gcaccaccca tcagtaccca tttggtaata ttgccgccgt gcacacggtg cagcaaatcc
    46741 tgcatcccca gcacccattt attaaaaata gggccgaaaa tgccaaaaat aataccgagg
    46801 atcagataaa gccacagcgt attaagcggc gcgtcagaaa gtttaccgac gtcaatcaac
    46861 gcaacttcat gattaaaaat ccggtacata atggtcgaca taatgacgcc aataaatacc
    46921 gctttaatcg aaattaacgt atagcgaaac tgcggacgca tctcttcgat aataaacaaa
    46981 atacccgcca gcggcgcgtt aaaggccgcc gccagacccg ccgccgcacc ggttgccaac
    47041 agcgtatggc gagcttcatc acctttcagg cggaaaacat cgagtaccat ccgcccgata
    47101 ttaccgccga tctgcacggt tggcccttcg cgccccaaca ccatgcctcc gccgagtgtc
    47161 cccagcccgc caaagaattt caccggcaat acacgccacc agcgaacagg acgttgatct
    47221 tccagcgccc cttcaatttc cgggatcccc gacccccctg cttcgggcgc gtatttgcgc
    47281 accaaaaagt agccgaacat cgccagcacc gccgaacaga gaaaagcaac ggttaacagt
    47341 agcggataat tatcagcagt atgtaccagc gcccccatac gttggttctg caaccaggcg
    47401 acacctttgt caaaagcaac cgctgccagc ccgacaagcg tgccgacgac tgccgccata
    47461 aacaaaatgg ctaacggggt tttatcgcgc tcaagaagtt ggcgaatcag ttgtctgcgt
    47521 cgcaggcgcg cggcctgcgg tgtttctaaa gagggagtat cagttttcat ccaatgatca
    47581 cttattggtc atacaaataa gatgacggca ttttactcgc acgttccatg aaaatccttt
    47641 gcaaagcgta atgtttcaaa tgcacaaaac ttttatactc taagaaatca ggattgcacc
    47701 agtacaagca gcctgatgtt tgacgagtat ttaacttgtt atgaataaca tagaatagca
    47761 gccattcact ttttctacga atcaggaacc ctccatgagt aagtctgaaa atctttacag
    47821 cgcagcgcgc gagctgatcc ctggcggtgt gaactcccct gttcgcgcct ttactggcgt
    47881 gggcggcact ccactgttta tcgaaaaagc ggacggcgct tatctgtacg atgttgatgg
    47941 caaagcctat atcgattatg tcggttcctg ggggccgatg gtgctgggcc ataaccatcc
    48001 ggcgatccgc aatgccgtga ttgaagccgc cgagcgtggt ttaagctttg gtgcaccaac
    48061 cgaaatggaa gtgaaaatgg cgcaactggt gaccgaactg gtcccgacca tggatatggt
    48121 gcgcatggtg aactccggca ctgaagcgac aatgagcgcc atccgcctgg cccgtggttt
    48181 taccggtcgc gacaaaatta ttaaatttga aggttgttac cacggtcacg ctgactgcct
    48241 gctggtgaaa gccggctctg gcgcactcac gttaggccag ccaaactcgc cgggcgttcc
    48301 ggcagatttc gccaaacata ccttaacctg tacttataac gatctggctt ctgtacgcgc
    48361 cgcgtttgag caatacccgc aagagattgc ctgtattatc gtcgagccgg tggcaggcaa
    48421 tatgaactgt gttccaccgc tgccagactt cctgccaggt ctgcgcgcgc tgtgcgacga
    48481 atttggcgcg ttgctgatca tcgatgaagt aatgaccggt ttccgcgtag cgctagctgg
    48541 cgcacaggat tattacggtg tggaaccgga tctcacttgc ctgggcaaaa tcatcggcgg
    48601 tggaatgccg gtaggcgcat tcggtggtcg tcgtgatgta atggatgcgc tggccccgac
    48661 gggtccggtc tatcaggcgg gtacgctttc cggtaacccg attgcgatgg cagccggttt
    48721 cgcctgtctg aatgaagtcg cgcagccggg cgttcacgaa acgctggatg agctgacaac
    48781 acgtctggca gaaggtctgc tggaagcggc agaagaagcc ggaattccgc tggtggtaaa
    48841 ccacgttggc ggcatgttcg gtattttctt taccgacgcc gagtccgtga cgtgctatca
    48901 ggatgtgatg gcctgtgacg tcgagcgttt taagcgtttc ttccatatga tgctggatga
    48961 aggtgtttac ctggcaccgt cagcgtttga agcgggcttt atgtctgtgg cgcacagcat
    49021 ggaagatatc aataacacca tcgatgctgc acgtcgggtg tttgcgaagt tgtgatgatt
    49081 ttgcctcgcc caccgggcga ggcattttat taacggctct gctttctcaa caaatagata
    49141 aaatatggcg cgccgataaa ggtcgacagc agccccgccg ggatctggaa cggaaacagc
    49201 accatccgcc cacaccagtc agcgaacacc agcagtaaac cacccaccag cgccgaaatc
    49261 acgatgtgcg gcatcgtccg tcgaaagccc atcatccgcg cgatatgcgg tgccattaaa
    49321 ccaacaaaac tcaacggtcc aatggtcatc gtcgcggtcg ccgtcaggca agccgctaac
    49381 agcagcagcg caattcgcgt cggcgttaac gcccccccca cggcccgcgc ggtatcacca
    49441 cccagcggta aaatggtcag ccagcggcgg cacagcgggg taatcgccag caaaatcacc
    49501 atcacaattc cggtgcgcca gacctgcgca tcggtggcgt tgtaggtcga accagagatc
    49561 caggtcagca cttgcgccat tcgcgggtca ccactcgcct gcaacatcat taaaagcatg
    49621 gtgaatgcgg tacttaacgc catccccgcc agtaacatgc ggtgtgggga aaatccaccg
    49681 cgcccggcgg cgatcataat aatcaacagc gtcaccgccg caccgatact tcccgcgggt
    49741 aacagccagc caaaagcatt acccggtacc agaaacagca tcagtaccac gccaaacgcc
    49801 gcgccagagc taatccccag cacttccggg cttgccatcg ggtttccggt cagccgctga
    49861 ataatacagc ccgccaccgc cagcatgacg cccgcaaaca gcgccgccat aattcgcggc
    49921 cagcgccagg gcatcaaatc ctcgagcaac gccccgctcg cccacgtcca gccgtgcgca
    49981 tcacgaccaa acgacagcgc caccaccaca gccatcaaca gcagcacgcc gcccgcgagg
    50041 gcaaacgcca gcacatgttg gcgttcagcc gcgacacgat cgttgacctt catatccggc
    50101 gcgctaatgc tgcgtaaacg cggcaacagc cacagtagca gcggcgcacc gatcaacgca
    50161 gtgaccgaac cggtggacac ttccatccac acgcgagtca gccagaggat gatttgatcg
    50221 gaaagccaga ggatcagcgc accaatcaac gacgccagca tcagtcgcgg cagcagacgc
    50281 cgcgccccca gcatttttgc cagcaacggc gcgaacaacc cgataaagcc gataatcccc
    50341 accgcgttca ccagcagcgc actgatgaca atagccagcg acagcgccgc cagacgcgcc
    50401 agcgacaagg ctagcccgag attgcgcgcc acgccatcat caagccccat cagtgttaac
    50461 ggacgaagta gcagcaacgt cagcatcaca ccgcccagca actgcggcca taaacgctca
    50521 acaccgcccc agtcggtttg cgtcagcgtt ccggtgctcc acaggaacat gctttgcagt
    50581 tggtcatggt ggaagataac cagtaactga ttgattgccc cacaataaag gctcactacc
    50641 agccccgcga ggatcagcgt taccggcgaa agccgtttcc cccacgcgac gccaaagacg
    50701 attaagccaa caacgcaagc gcccgccagc gcagcaaact gactcgccat cgcccctggg
    50761 atcgcccaga gcgtagtgac ggtaatcccc agttgcgcgc ctgtagcaac gccaagtgtc
    50821 gtcggttccg ccagcggatt acgcagcact tgctgaaaca gcacgcccac cagccccagg
    50881 cctgcgccca ccagcagcga aatcgccaga cgcggcagca agctgtagtg aaaaatcatc
    50941 tgctcgatga cgtcaatatc cggcgaccag gcagcctgcg cccactggct acgcggcagc
    51001 gcctgcgaga agttcatcca ggtcagcgcc gtagcggcaa tcaccagcaa cgccagcaat
    51061 aatgccggga aaagcgcaat acgtttactc acgctttacc tccaatggcg ttatccagaa
    51121 cgcgcacaaa gtgcattgcc gagagagttg caccataaaa ccagactgcg ggtacgcgct
    51181 gaaagcgtcc ggcgcggaca aacggcatgg cctgccacag cggcgttgcc attagcgcat
    51241 ccatgtcttt gctgttgtcg tgatcaaaac agagcacatc aacgtcttta tacgctgcca
    51301 gacgatcgat gctgacggcg gtactgcccc agaagttggt ttccccttgc caggcatttg
    51361 ggatgccgta ctcatcaaga atttcctgga acaagctgtt tggaccgaag accagcatat
    51421 ggcgcggatc gataagcgtc gtcagcaata acggacgcgc accacgcttc acaaagcggg
    51481 ggttcatgct gcggataaag tcttcatatt gcgctaaatg cgtttctgct gcgctttgca
    51541 ggttaagtaa atccgccatt tccgtcagcg atttacgcgc catcgccagc ggctgtttgc
    51601 cgtcgctgaa gttaaatcca cgccccggcg caatacgagc cagcatttct ggcgaaggac
    51661 catatcccgc cgaccagacc ataaacgacg gtttcatttc ggtcagcagt tcgaggttag
    51721 gttctgtacg caaaccgacg tcaatcaccg attcaggtag tggtggttcg ctgacccaca
    51781 gtcgatagtt gatggtgtct gccacgccat aaggcacgat gccgagcgcc agtagtaatt
    51841 ccaccggcag ccactccagc gccacaatac gatttgggtc aatagccgcc gcgtgggcgg
    51901 tattcatctg ccataacaac ggagaaagcg ccatcgccgt taacagtcga cggcgcgaaa
    51961 taagaggtaa gccgctcatc aataaacaaa actcacaggt gcagcacccg ccggatgcgg
    52021 cagaataccc atcgggatgc cataaatcat ttcgagggtt tcgccgcgca taatttccgc
    52081 aggcgttccc tgagcaatca tttcaccgcc gcgcagggcg accagataat cacagtaacg
    52141 cgcagccata ttgatatcgt gcagcacggc aatgaccgtc aggccacgct cctgacttaa
    52201 acggtgcacc agcgccagca catcaacctg gtgggcgata tccaacgccg aagtcggttc
    52261 gtcgagcaac agacaacggc tatcctgcgc caccagcatg gcgatccacg cccgctgacg
    52321 ttcgccgcca gagaggctgt ctaccagccg atgcgccagc ggttttaagc caaccagcga
    52381 gatagcttcc tcaacttttt cgcgatctgc cgcaccaaag cgccctagtg cgccatgcca
    52441 cgggtaacga ccaatcgcca ccagttcacg cacggtcatc ccttctgccg gaggaagctg
    52501 ctgcggcaaa taagccactt tgcgggcgaa cgctttgctg ctccagcttt ccagcggctg
    52561 ggcatcaaga agaatctccc cttccgacgg cggctgatga cggccaagca ttttgagcag
    52621 agtggattta ccagaaccgt tgtgaccaat cagaccggtc actttcccgg caggaaaggt
    52681 taacgacagc ggatgcaaaa gcgtgcgccc gggcacacga aaggagatat tacgcagtgc
    52741 aaaagtggta tcggaatgat tcgtgtattc ctgcataaca gccaacttgt gaaacaggca
    52801 cggaaatccg tgccccaaaa gagaaattag aaacggaagg ttgcggttgc aacgacctga
    52861 cgttctgcgc cccagaagca gccataagtg ttaaagcagc tggcgacgta ttcacgatcg
    52921 aacaggttgt taacatgcag cgccacgttg gagcccgcca tgccgactcg cgccagatca
    52981 taacgcacta acgcatccac gaccgtataa cttcccactt taaaggagtt agccggatca
    53041 ccatagctgg agccagtata acgaccaccg gtgcccagcg tcagacctga aagcggaccg
    53101 tcaaagaagg tatagtcagc ccacagcgaa gccatgtgtt ttggcacctg tgcaggcgta
    53161 ttgcctttat aggtagtatc ggtggtgtat tccgcatcgg tgtaagtata agaaccgact
    53221 acgttaacac tcgccgacag cgccgctttc gcttcgattt ctacgccacg tgcgcggatc
    53281 tcgccacctt caaccgagaa gaaggaaccc tcagggtccg ccatcaggtt gttggtttta
    53341 gtgagattat acacggcacc agtaactaca atcggacgat cttccggtac atatttcacg
    53401 ccgacctcat actgcttacc tttagacggt gcgaaaatat taccatcctt cccaacttgc
    53461 gaagaaggtt caaacgattc gctatagcta aagtaaggtg ttacgccatt atcaaacagg
    53521 tagttaacac caccacgcca ggtaaactgt ttgtcatcac gtttatcggt cgtcccggca
    53581 acgcggttaa gagattcttg atctgcccag tcataacgac cgcccagggt gaccagcact
    53641 ttatcccact gcgcctgatc ctgaacataa acgcccgttt gtttctgctt attcagaatg
    53701 cggtaagggc cggagtttgc cggatctttg gcattgaagt cgaaatcggt attcaccgga
    53761 ttgtacagat cgagcagcgg tacggagtcg tcgtaaccaa accaggcgtt gatgtcatta
    53821 cgcatacgca taaagtcgac accggtcagc agggtgtggt cgatatcgcc agtggcaaac
    53881 ttgctctgca actgggtatc aacggagaag ttttgcagct tctcatcatc aacgacgtat
    53941 ttacgtgcca gataatggcc tttatccgct ggcgctaatg ccgcacactg tttgctgtaa
    54001 gcattcgccg gatcggagca gacgccgtaa ccataaacgc tgttttgcga ggttttgttt
    54061 tcagcaaagc gcaggttctg acgcacagta aaggtgtcgt taaattcgtg atcgaagctg
    54121 tagcccacca tcttctcatt acgagaatag gtgttgttct tcgccccttc gttaaagtct
    54181 gtcggcagac gcttaccgtt cggcagcggc tcaacggttc cctctttcgg caaccagccg
    54241 taataaccgg tttccggctc gttctggaag taagaaagga aggtgaaatt ggttttatca
    54301 tccggacgcc aggtgaacgc cggtgcaata gcataacgct gctcttctga ccctttctgc
    54361 tgggcattgg cagaacgcgc aagaccggtc aggcgataag agtaaacgcc gtcatcatcc
    54421 agcgcatcgc taaagtcaaa accagtctgg aacaggctgt cagtaccggc tttaaactga
    54481 acttctttca gcggttcggt ggtcggacgc ttgctgacca tattcaacag gccgccagga
    54541 ctgcttttac cgtaaagcac ggaaaccggg ccacgcataa tttcagcgcg ttccagcata
    54601 tacggatcaa tgaccgcatc gttatagaag ttgccctgca acttcaggcc attcagatag
    54661 ttattctggc tttggccttc tgccgcaaaa ccgcgaatga tcaggtggtc ataggtgttg
    54721 gatgcgccac gcgtaccaac agagacaccc ggcgtgtagc taagcgcttc ttttaccgac
    54781 ttcggctgat gcagcgccat ctcttcggcg gtcacaacag aaatagactg tggcactttt
    54841 tgaatcggcg tatcggtttt agtgccggta gctgactgtc gcgccgcaat agttgcagca
    54901 ggcccccatg cgctttcttg cggcgcaggt gcagcggtaa cggtgatagt gtcttctttc
    54961 ggttcaaccg ctgcctgtgc ataaacagac atgccgctaa ccgctgtggc tactacaact
    55021 gcgattttac gcagtgagtg ttttggctga gcagttttgg aacgcgccat tggtatatct
    55081 ctgatgtaaa gtgaatgata acgtaaacga gaatgattat tataaagata agataatatt
    55141 cgaaactctg gcgggttcgc aagcggtacg caatctgagc gttcctgaag ggttaataaa
    55201 caaccagatg aaaagaaagg gttaatactt tgggtgggat gttattttac cggatggcaa
    55261 ctcgccatcc ggtatttcac gcttagatgt taattactac caaacatatc cttaatccaa
    55321 ccggctacac cgtcgctgtc tttctgctct tgctgagcag gttgctgttg cggctgttgc
    55381 tgcggctgag aagactgatc aaacggattg cctgacggct gctgctgcat ctcactctgc
    55441 tggcacagcg attgcggatc gctggtccag accggcaaga cacgcatgcc accgctgcaa
    55501 acaaagttgc cgtcgtagtc cacgcccata tctgcaatat cttctggcgg aacaagattc
    55561 agcggcgttg gcgtctggtt agccagataa cgctgataaa tcgacattgc cccgctggct
    55621 ccatacagtt tggtcggctg gttgttatca cggccgaccc aggtgatggt caccgtgctg
    55681 ccgtcaatgc ccgcaaacca ggtatctacg ttattgttgg tagtccctgt tttccctgcc
    55741 agatgcaggt tcgggtattt cgccccaagc tgacgacccg taccgcgttg taccacctgc
    55801 tgcatggtcc atagtgtcag atacgccgcc tgcgccggaa cagcgcgttc cgcctgcggg
    55861 aagctctgat acagcacttt gccatcttcc gcgattaccg aacgcagcgc agaaagcggt
    55921 gcacggttac caccgctggc gatggtctgg aatgcctgcg ccacttcgat tggcgttaag
    55981 ttcaacgccc ccagcagcat tgccggaacc ggatgcaact gatctttcgg tacgcccagt
    56041 ttaatccagg tatccgtaac cgcaggcagc cccagcgcca tccccagatt taccgtcggc
    56101 acgttcatcg aacgggtcaa cgcatccacc agcatcactc tgccgctttc gctataacga
    56161 cggtcatcat tctgcggtga ccagacctgg ccattcggct gacgcagcgc aattggcgca
    56221 tccgcaatcc acgtattcag acgatagatt ttcggctggc ttaaggccgt cagataagtc
    56281 gctggttttg caagggaacc aatcgaacga cgcgcctgca tcgcacggtt gtagcccgca
    56341 aactgcggct cagaacctcc gaccatcgca cgaacttcac cactaaagcg gtcgacgacc
    56401 acaatcgcag tttcaagatc gctcaactta cgctgtttct tcagtgccgg aataccttcc
    56461 acggcggctt tttctgccgc gtcctgggcc accgagtcaa aggtagtgaa gatcttcacg
    56521 ccggagagat cttttacctt atcgcctaat ttagcctgaa gctcctgacg caccagttgc
    56581 ataaaggctg gctgaggaga gatcacccca ccgcgcggct gaacccccag cggacgcgca
    56641 ctcaacatgt catagagttc ttgatcaata atctgttgct gttgcagcag acgcagtacc
    56701 agattacgtc gctccagcgc cagtttcggg ttacgccacg ggttgtagat ggacgctcct
    56761 ttcaccatac cgactaacag cgcctgctga tcgaggctca attcttctac cgggcgacca
    56821 aagtaataca agctcgccag cgggaagccg cggatttcgt tgtcgccgct ctgaccgaga
    56881 tacacctcgt tcatatacag ctcaagaata cggtctttgc tgtaacgcgc gtccatgatc
    56941 agcgccatgt aggcttcatt cgctttacgc caataagagc gctcgctgga gaggaacagg
    57001 tttttcacca gctgttgcgt cagcgtactc gccccctgca ccgtgcgacc tgcggtcagg
    57061 tttgccagca ccgcacgtcc gatggagtag agactgattc catcatgctc gtagaaatgg
    57121 cggtcttctg tcgccagcaa agtatccacc agcaaatccg ggaaaccact gcgcggcaca
    57181 aacaaacgct gctcaccgtt tggtgaggag atcatggtga tcagacgcgg atcaagacgg
    57241 aagaaaccga actgacggtt gttctccata ttgacgatgg tcgccagatg atcgccatca
    57301 aaggtcagac gcgcgcgcac ctgcccttct ttactgtccg ggaaatcaaa cggacggcga
    57361 atcatctcaa tgctgttggc ctgcacggta aattcgccag gacgggtcat tttcgacacc
    57421 tgacgatact gtgttgcctc cagcagcttc accatctcgt tcttgctgat ggtcatgtct
    57481 ggctcaaggt tgaccattcg accataaact gccgcaggca gttgccagac cttgccatca
    57541 atacggctac gaattttttg atcgagataa acgccgtaaa tggcgatcag cacggcaaaa
    57601 acgatagcca gttttaacag cagccatagc cagccgcgtt tgccacgagg cttacgccct
    57661 ttgcctttgc ccttaccttt gcgcggcatc ggttcttcat cctcatagtc atcataatcg
    57721 tcgtaatcat cgtcatcttc gtaacgacga cggcttacct tttgtttgac cggacgcgtc
    57781 ggtttccctt tgcgtccaat tggctcgcgg tcattcccgg ccatgctttt tctccgcaat
    57841 attcaggcgc aaaagcccga ttttctgttc ttctgttaca agacagaaga agatatctct
    57901 caaaatttga gctcgatcgg tcccctctcc cttaagggag agggttaggg tgagggtgaa
    57961 cgtgcgcgct ggtgccctca ccccggccct ctcccacagg gagagggaga aaaccaacct
    58021 tacgaatact ttttcgtccg tcgcgtcggt gccgtgtttg ccgggtcgtc cggccagacg
    58081 tgtttgggat aacgcccttt catctccttt tgcacctcac gatacgcccc tttccagaag
    58141 gcccccaaat cacgcgtgat ttgcagcggc ctttgggcgg gtgaaagcaa ttccagcacc
    58201 agcggcacgc gcccctgggc gatcgtcgga ttagtggcct caccaaacat ctcctgcatt
    58261 ctcaccgcca gcgcgggcgg gttatcttca tgataacgaa tggcgatccg gcttcccgtc
    58321 ggcacagtgt aatgcgcagg caattcacta tccagacgtt gctgcattcc ccaatcaagt
    58381 aatccgcgta gtgcctgata aatgtcgagt gatttcaggc cgcgtagtga atgtacaccg
    58441 gccatatgtg gcagcagcca cgtttccagc gtcgccaaca aactttcatc atcaaccgct
    58501 ggccagtcat attctggcaa ccactttccg gcgcataaca aacgcaagcg tagctgttcc
    58561 gcttccgccg tccagttgag cacgcttaaa cctttatcac ggatgccgtt aagcatcgcc
    58621 tgatgcaact cgtcttctga cggtttcgcc aacggctgca ctttcaccgt caactgaccg
    58681 atttgcagcc gacgccaggc tttcagcgta ccttgcgcgt catcccattc cacggtgtca
    58741 gattgctgca ccagctgcgg acagcgttgt actaattcat caatatcgac cggtaacgcc
    58801 agtaaaatcc gcgcatccgg cgaggcgctg ccctgcaata ataacggcgc gatcaaccat
    58861 tcgtggcgac ttagcgcgtc gtcggcatca agcatcgccc ccatgccgtt tgccagttga
    58921 tagcgtccat cttgcccacg gcggcgagca atgcgatcgg caaacgcccc ggcaagtagc
    58981 ggcgcgataa gcgaactgtc tgcctcaccg ccgcgtacgt ttaagcgttt taacagttgc
    59041 tgactacgtt gctgccaggc tggttgattg cgcgaaaacg ccacgcccag gtcactattg
    59101 cccatccgtg gcggctcttc gagaatggcg gcaatttttg ccgcggtagc agcttcgtcg
    59161 tcgctctttg cgctaaccag cattgccgct aaacgcggat cgttacccag cgctgccatt
    59221 ttttgccctt gcgcactaag ccgttcaccg tccagcgccc ccagcatttg taacagacgt
    59281 ttcgcggcca gtagattcac cgttggcggt tgatccagcc agctcatctg cgccggatcg
    59341 ctgcatcccc attgcagtaa ttccatcagc aaaccggaaa gatcgctttg taagatctcc
    59401 ggttcacttt gcgcggtagc gcgttctgct tgttctttgg cgattaaatg caggcagata
    59461 cccggctcca gacgcccggc gcgcccggca cgctgcgtca tcgacgcctg actgattcgc
    59521 tgagtaatca accgcgtaag ccctgttcgc ggatcaaaac gcgccaccct ttcctgggca
    59581 caatccacca ccagacgaat accttcaatg gttaaactgg tttcagcaat attggtcgcc
    59641 agcaccactt tgcgcatccc ttgcggtgcc gggaggatcg cttttcgctg atcgttcagc
    59701 gacaacgcgc catacagcgg gcagagcaat acatcactgc cgatgcgcga agccagttgt
    59761 tcctgcacac gctgaatttc tccgacgcca ggtaaaaata acagtaatga tccgctttcc
    59821 tgacgcagca tttcagcggt ggctaccgca acggcatcgt caaaacgctg atgcgtgggc
    59881 agcggtaaat aacggcgttc aaccggaaac gagcgccctt ctgagatgac gacgggcgct
    59941 tctggcagca tttgctgcaa gcggtcgttg tccagcgtag ccgacataat cagcagttta
    60001 aggtcatcac gcagaccttg ttgcacatcg agtaacagcg ccaacgccaa atccgcctgc
    60061 aagctacgct catgaaattc atcaaggatc accaatccaa caccgctcag ttccgggtca
    60121 cgctggatca tccgcgtcag cacgccttcg gtaactactt ccaggcgggt attcggcccg
    60181 acgcagtttt gcgcacgcat ccggtagcca acggtatcgc ctggcttttc gttaagcagc
    60241 tccgccagcc gttgcgcgac gttacgcgcc gccagacgcc gtggctccag gaggataatt
    60301 ttcccgttaa tgccgggatg cgccagcagt tgcagcggca gccaggttga tttcccggcc
    60361 ccggtcggcg cacttaataa tacctgcggc gcataatcga gagcggtaag taattcaggt
    60421 aacacggcag caacgggcaa cgacgacaca aaacgctcca gagggttaac attcttcgcg
    60481 ccacattgta gcatcgcggt aattcataac cgagtgcctc acatgtctga cccgcaacgt
    60541 ctgttctttg ctatcgactt acctgcagaa atccgcgaac agattatcca ctggcgcgcc
    60601 acacacttcc cacttgaggc gggacgtccg gtcgccgccg ataatttgca tctgactctg
    60661 gcatttttag gcgaagtgag cgcagagaaa gagaaggcgc tttctctttt agccggacgg
    60721 attcgtcaac ctggtttcac actcacgctt gatgacgccg gccaatggct gcgttcgcgt
    60781 gtggtgtggt tagggatgcg tcagccgcca cgcggcttaa tccagctggc gaatatgctc
    60841 cgttcacagg ctgcccgcag cggttgtttt caaagcaatc gtccgtttca tccacatatt
    60901 accttattgc gcgacgccag cgaggcggtg acaatcccgc cgccaggttt taactggtca
    60961 tatgcggtga cggagttcac cctttacgcc tcctcgtttg cccgtggacg cacacgctac
    61021 acgccgctaa aacgctgggt gctaacgcaa taacaaggat tgtcgcaatg gaattttctc
    61081 cccctctaca gcgcgcgacg ctaattcagc gttacaaacg ttttttagcc gatgtgatca
    61141 cacccgatgg tcgcgaatta acgctacact gcccgaatac gggtgcgatg accggttgtg
    61201 caacgcctgg cgataccgtc tggtattcga cttcagacaa caccaaacgg aaatacccac
    61261 acacctggga attaactcaa agccagagcg gcgcatttat ttgcgtcaac acgctttggg
    61321 ctaacaggtt gacgaaagag gctatcctta atgaatcaat ttcagaactg tcaggctata
    61381 gctcgctgaa aagcgaagta aaatacggcg cagaacgcag ccgtattgac tttatgttgc
    61441 aggcggattc gcgtccagac tgctatattg aagtgaaatc ggttacgtta gcggagaacg
    61501 aacagggata ttttcccgat gcggtcactg aacgaggtca gaaacacctt cgggagttga
    61561 tgagcgtagc ggctgaaggc cagcgtgcgg ttatcttttt cgccgtgctg cattcagcca
    61621 ttacacggtt ttcacccgcg cgccacatcg atgagaaata cgcgcaacta ttgtcagaag
    61681 ctcaacagag gggggtagaa attctggctt acaaagcgga aatttctgct gaaggcatgg
    61741 ctcttaaaaa atcactgccg gttacattgt agtaaagtaa gtaactggtt aatttacatt
    61801 ctggtcgcgt gcgcaaatac gcttttcctc acacagttgt caagtgttac gtttagataa
    61861 ttgctatccg gaaaagcatc tgctatttat agcggcctca tttttccccc gaacatgggg
    61921 atcgatagtg cgtgttaagg agaagcaaca tgcaagaagg gcaaaaccgt aaaacatcgt
    61981 ccctgagtat tctcgccatc gctggggtgg aaccatatca ggagaagccg ggcgaagagt
    62041 atatgaatga agcccagctg gcgcacttcc gtcgtattct ggaagcatgg cgtaatcaac
    62101 tcagggatga agtcgatcgc accgttacac atatgcagga tgaagcagcc aatttcccgg
    62161 acccggtaga ccgtgcagcc caggaagaag agttcagcct cgaactgcgt aaccgcgatc
    62221 gcgagcgtaa gctgatcaaa aagatcgaga agacgctgaa aaaagtggaa gacgaagatt
    62281 tcggctactg tgaatcctgc ggtgttgaaa ttggtattcg ccgtctggaa gcgcgcccga
    62341 cagccgatct gtgcatcgac tgcaaaacgc tggctgaaat tcgcgaaaaa cagatggctg
    62401 gctaattaca gccgttccat cacgtttacc acaggcggga aatgctcccg ccttattttt
    62461 tgttcaaaga gatgacagac acacagtata ttggccgctt cgccccctct ccttccggcg
    62521 agcttcattt tggttctctg attgctgcgc ttggcagcta tttgcaggct cgcgcccggc
    62581 acggtcgctg gctggtacgc attgaagata tcgacccgcc tcgtgaagtt cccggtgccg
    62641 cagaaactat cctgcgccag ctggaacatt acggtctgca ctgggacggc gatgttctct
    62701 ggcaatcgca acgtcaccac gcctatcgtg aagcactcgc ctggttacat gaacaaggac
    62761 taagttatta ctgcacctgc acgcgtgcgc gtattcaaag cattggcggt atttacgacg
    62821 gtcattgccg ggtgttgcat catggaccag acaacgccgc agtgcgtatc cgccagcagc
    62881 atccggtcac gcaatttact gaccagctgc gcggcattat tcacgccgac gaaaaactgg
    62941 cacgggaaga ttttattatt catcgccgtg atggtttgtt tgcctataac ctggcggtgg
    63001 tggtggatga tcacttccag ggcgtgagcg aaattgtccg cggggcagat ttgatcgaac
    63061 cgacagtgcg gcaaatctcg ctgtaccagc tttttggctg gaaagtgcca gattacattc
    63121 atctgccgct ggcgcttaat ccacaaggcg ctaaactttc caagcagaat catgcgcctg
    63181 cgttgccgaa aggcgatcca cgcccggtac taatcgcggc acttcaattt ctggggcagc
    63241 aggcagaagc acactggcag gatttcagcg tcgagcaaat ccttcagtca gccgtcaaaa
    63301 actggcggct taccgcagtg cctgagtcgg caattgtaaa ttcaacattc tcaaatgcgt
    63361 catgctgagc tatgattagc cgctattttt ttgtcctgaa tgatgtttga cactaccgag
    63421 gtgtactatt tttacccgag tcgctaattt ttgccgcaag gtgctaagcc gcgaggaaag
    63481 cgaggctgaa caggcagtcg cccgtccaca ggtgacggtg atcccgcgtg agcagcatgc
    63541 tatttcccgc aaagatatca gtgaaaatgc cctgaaggta atgtacaggc tcaataaagc
    63601 gggatacgaa gcctggctgg ttggcggcgg cgtgcgcgac ctgttactcg gcaaaaagcc
    63661 gaaagatttt gacgtaacca ctaacgccac gcctgagcag gtgcgcaaac tgttccgtaa
    63721 ctgccgcctg gtgggtcgcc gtttccgtct ggctcatgtg atgtttggcc cggagattat
    63781 cgaagttgcg accttccgtg gacaccacga aggtaacgtc agcgaccgca cgacctccca
    63841 acgcgggcaa aacggcatgt tgctgcgcga caacattttc ggctccatcg aagaagacgc
    63901 ccagcgccgc gatttcacta tcaacagcct gtattacagc gtagcggatt ttaccgtccg
    63961 tgattacgtt ggcggtatga aggatctgaa ggacggtgtt atccgtctga ttggtaaccc
    64021 ggaaacgcgc taccgtgaag atccggtacg tatgctgcgc gcagtacgtt ttgccgccaa
    64081 attgggtatg cgcatcagcc cggaaaccgc agaaccgatc cctcgcctcg ctaccctgct
    64141 gaacgatatc ccaccggcac gcctgtttga agaatcgctt aaactgctac aagcgggcta
    64201 cggttacgaa acctataagc tgttgtgtga atatcatctg ttccagccgc tgttcccgac
    64261 cattacccgc tacttcacgg aaaatggcga cagcccgatg gagcggatca ttgaacaggt
    64321 gctgaagaat accgatacgc gtatccataa cgatatgcgc gtgaacccgg cgttcctgtt
    64381 tgccgccatg ttctggtacc cactgctgga gacggcacag aagatcgccc aggaaagcgg
    64441 cctgacctat cacgacgctt tcgcgctggc gatgaacgac gtgctggacg aagcctgccg
    64501 ttcactggca atcccgaaac gtctgacgac gttaacccgc gatatctggc agttgcagtt
    64561 gcgtatgtcc cgacgtcagg gtaaacgcgc atggaaattg ctggagcatc ctaagttccg
    64621 tgcggcttat gacctgttgg ccttgcgagc tgaagttgag cgtaacgctg aactgcagcg
    64681 tctggtgaaa tggtggggtg agttccaggt ttccgcgcca ccagaccaaa aagggatgct
    64741 caacgagctg gatgaagaac cgtcaccgcg tcgtcgtact cgtcgtccac gcaaacgcgc
    64801 accacgtcgt gagggtaccg catgacagtg gcgtatattg ccataggcag caatctggcc
    64861 tctccgctgg agcaggtcaa tgctgccctg aaagcattag gcgatatccc tgaaagccac
    64921 attcttaccg tttcttcgtt ttaccgcacc ccaccgctgg ggccgcaaga tcaacccgat
    64981 tacttaaacg cagccgtggc gctggaaaca acacttgcgc ctgaagaatt acttaatcac
    65041 acacagcaaa tagagttgca gcaaggtcgc gttcgtaaag cagaacgctg gggaccgcgc
    65101 acactcgatc ttgatatcat gttgtttggt aatgaaacca ttaataccga acgactcacc
    65161 gtcccgcatt acgatatgaa gaatcgcggc tttatgctgt ggccgctgtt tgagattgcc
    65221 ccggaactcg tgtttcctga tggggagaca ttacgtgaaa tactcacttc gctagcagta
    65281 ctacaacctg ccatctggta ttagctgtat tattttattt tcttatctta ctcctcactt
    65341 cccctcttac agattaaata tacttaattt acattttagt tttttttaat taattcatga
    65401 aatagcactt catgcaaata gattaggcat tttttgttta aaaaacctag aatagcaaca
    65461 attcaataca ccacgtaaat atattcacac ccgtgataat tcacccctat tacatcctcc
    65521 aaaaactttg agtaatatcc gctcgcaata ttaatttcat ataatttttg taaggatgca
    65581 tgtaatgaaa aaagcacttc tcgcagccgc tctggttatg gcttctggtt ccgccctggc
    65641 tgtagatggt ggtcatatcg actttaacgg tatggtacag tccggtacct gtaaagtggg
    65701 tgtggtagat actggtatgc atagcgttac cactgatggc gtggttaccc tggatactgc
    65761 gaatgttact gatacttttg ctgaagttag cgcaactgct gtcggtttac tgccgaaaga
    65821 gttcatgatt tctgttgagt gtgatccagg tgctccgaag aatgctgagt taactatggg
    65881 ttctgcaagt tacgcgaaca ccagcggtac cctgaataac aatatgaaca tcactgttaa
    65941 cggtattgca ccggctcaga acgtaaacat tgcagttcat aacatgaaaa acaaagctgg
    66001 cgctgctgaa attaagcagg tccatatgaa caactcttct gaagttcagg aactgacatt
    66061 agacgcagaa ggtaaaggcc agtacgtatt taacgcatct tacgttaaag caccgaacag
    66121 cccggctgta actgctggtc atgtaaccac taacgcgctg tacaccgttg cttataagta
    66181 attgatggtg tgttgagcat aaggtttgcc agcaggcaaa ccttattttc gcgaatttaa
    66241 tctttctaca gacagcaagg agtgcttatg tttcccttgg ttaaaaaaac ggttagtgca
    66301 ttatttgtga gtactctgtt agccagtgca ccagcattcg ctgacattat tatttccgga
    66361 acacgtatca tttataacgc tgataaaaaa gatgttaacg ttcgtctgga aaataaaggg
    66421 aatcgcccct tactgattca gaactggttg gatacgggcg atgacaatgc cgatcccagc
    66481 cagatcaaag tgccgtttac cgcgacccca ccagtttcac gcgtggagcc aaaacgcggt
    66541 caaaccgtaa aagtgatgta taccggtgca acagccctgc cgaaagaccg tgaaagcgtc
    66601 tactggttta acgtgctgga agtgccgcct aagccgaaag atgctgaggc tgacaaaaac
    66661 ttgttacagc tggctttccg cacacgcatc aaactctttt atcgcccgag tgggttacag
    66721 ggagaaccgg ctgaagcacc ggcgaaaatc acctggaaat taaacaacgc tcagttacag
    66781 gcaaataacc caacgccata ttacgtgtcc tttaatgagg tcaagctgga aagcggcggc
    66841 aaaacctaca acgtcaattc cagcatggtc acgccgttca gccaggcaag ctttggcgta
    66901 accagtctgc caggaagcgt gtcatccgga aaagtggtct ttaaagcgat caacgacttt
    66961 ggcggcaaca ttgatggttc cgcaacattc taaaaaataa acggattctt tttaacatcg
    67021 ccaggaatgc aaccttgtac cagtttactc atcaaaaaag ccgtatcccg aaaaaaacgc
    67081 tacttgcggc ctgttgtgcc ctgttttata gcagcaacgg tgctgcggcg gacaccgtgg
    67141 aatatgacag ttccttttta atgggaactg gcgcatcaac gattgatgtt aaacgttatg
    67201 ctcaaggcaa cccgacaccg ccgggtctct ataatgtccg cgtatttgta aacggtcagg
    67261 cgacttccag cttagaaatt ccgtttgtgg atattggcga aaacagtgcg gcggcctgtc
    67321 ttacccataa aaacctggcg caacttcaca ttaagcaacc tgaacagcct gtcactttac
    67381 tcgccagaga aggtgaagaa gaggattgtc tggatctggc aaagtcatac gaaaaggcgg
    67441 atgtgtgctt tgacggtagt gaccagtttc tcgatctgac gatccctcag gcctatgttc
    67501 tgaaaagcta tggcggctac gttgaccctt ctttatggga atcgggaatt aacgctgcca
    67561 cactggcata taccctgaac gcgtatcaca caagttcaga taacgacaat agtgacagcg
    67621 tctatggcgc gttcaactca ggtatcaatt taggagcctg gcactttcgt gcgcgcggta
    67681 actataactg gacaacagat aacggcagcg atttcgattt ccaggatcgt tacttacagc
    67741 gtgacattcc ggcaatccgt tcccagataa ttatgggtga tgcctatacc accggtgaaa
    67801 cgtttgactc tgtcaacgtc cgtggtgttc gcctgtacag cgacagccgt atgctgcctt
    67861 cggcgctggc cagttacgct ccgaccatcc gcggtgtagc aaactccaac gccaaagtca
    67921 ccgtgacgca aagcggatat aaaatttatg aaaccaccgt tccgcccggt gaatttgtta
    67981 tagacgacat tagcccttcc ggctttggta gcgaactggt cgtgaccatt gaagaagcgg
    68041 atggttccaa acgcaccttt acgcaaccct tctcgtcggt tgtacaaatg caacgtcctg
    68101 gtgtgggccg ttgggatttc agcgcgggta aagtcattga tgacagtctg cgatccgaac
    68161 ccaatatggg gcaagcctct tattactatg gtctgaataa cctcttcacg ggttataccg
    68221 gcattcagtt caccgataat aactatcttg ccgggctgtt aggtgtgggt atcaacacca
    68281 gcatcggcgc ctttgcggta gacgttaccc attcccgtgc tgaaattccg gatgataaaa
    68341 cctaccaggg gcaaagttat cgcgtgacct ggaacaaact tttccaggat accgggacat
    68401 catttaacct cgcggcgtac cgctattcca cccaggatta cctgggcctg catgatgcgt
    68461 tagtcctcat tgacgacgcc aagcatttgt ctgccgatga agacaaaaac accatgcaga
    68521 cgtactcacg tatgaaaaac cagtttaccg tcagcattaa ccagccattg aatatcgcct
    68581 atgaagatta cggttcgctg tttatttccg gtagctggac gtattactgg gcggcgaaca
    68641 atagccgcac tgaatataat gttggttaca gtaaaagcgt ttcgtggggc agtttcagcg
    68701 tcaacctaca acgtagctgg aatgaagacg gcgagaaaga tgacgcgatg tacgtcagcg
    68761 ttagcgtacc tattgagaat attttaggtg gcaaacgtaa gtcttctggt ttccgcaatt
    68821 taaatactca gctcaatacc gatttcgatg gttcacatca gttgaatgtt aacagttccg
    68881 gtaacactga aaacaatctg gtgaactaca gtgtcaacgc aggttatagc ctcgataaaa
    68941 acgccggcga tttagcctct gttggtggtt atctcaacta tgaatctggg ttaggcggta
    69001 tttccgcttc ggcctcggcc acttctgata acagccaaca gtactccatc tcaaccgatg
    69061 gcggctttgt attacacagt ggtggtttaa cgttcactaa caacagtttc agcagtaacg
    69121 acacgctggt gttaatcaac gccctaggtg ctaaaggcgc acgaatcaat aacagtaata
    69181 acgaaatcga tcgctgggga tatgccgtga cgtcctctgt cagcccatat cgtgaaaacc
    69241 gggtaggtct gaacattgaa acactggaaa acgatgttga actgaaaagt accagcgcca
    69301 ccaccgtacc acgtagcggc tccgttgttt tgacccgttt cgaaactgac gaggggcgtt
    69361 ctgccgtgct gaatattact gccgccaatg gcaaatccat tccgtttgct gcggaggttt
    69421 accagggtga ggtgatgatc ggcagcatgg gccagggtgg tcaggcattt gtacgcggta
    69481 ttaacgacag cggggaatta atcgtgcgct ggtatgaaaa caaccaaacc attgactgta
    69541 agttgcacta ccagttcccg gcgcagccac aaacgcaggg aagcaccaac accttattac
    69601 ttaacaatct tacctgtcag gtagcaaatc actaatatgg aaaattctat gaaaaggata
    69661 ctactaacat ccgcgttaat aggcctgggt ttaccggcgg ttggctctgc aacagatctt
    69721 aatgtcgatt ttacggccac cgtattagcc accacctgca cgataactat agtggaagat
    69781 ggtggtccag cagtgaccgg tagtaacgat gaatacagcc tgacaattcc tgatgttggt
    69841 ctggataaag tcgctaccgc tgcgcccgaa gctcaggcca actttaaatt aaaggcctcc
    69901 gattgcagca atggttactc aaagattttt accacactta caggaacaac ggtttccggt
    69961 aagttaattg ttaatgaagc aacctctggc gctgctgggg tggggatggg gattaagcgt
    70021 agagataccg cagactcgac tttcttcaca cccaataata ctgacaaatt tgaatggagc
    70081 gccgatgaga aagccagcgg tgtgcctctg accgtagcac tgcgcgaaac aacggcggga
    70141 gcaggacgca ccggtgcgtt tcaggcaaaa gccaccttta acttcaccta cgaataacag
    70201 ggacagccac catgaaatta aaagtcatcg ctacactgat tgctactgtt gccgtgggtg
    70261 taagctttaa cagcaatttt gcttctgcga gtacaacgtc cgcttcttta accgtaaaca
    70321 gtaacctgac tatgggtacc tgcagtgctc agataatgga taatagtaat aaagtgatca
    70381 atgaagtggt ctttggcaat gtttatattt ctgaactcgg tgcaaaaagc aaagtgcaac
    70441 agtttaaaat tcgctttagc aattgctctg gccttcccca aaacagcgcc caaatagtgc
    70501 tggcacctaa tggtatatcc tgtgctggtt ctcaatcgtc atcggcgggt ttttctaaca
    70561 agtttactga cgctagcgca gcaaccagaa cggctgtgga agtatggact acagatacac
    70621 cggaaagcaa tggcagtacg caattccatt gtgctcaaaa gataccagtg cctgtgacgc
    70681 ttcccgccga caccacaact cagccttacg attacccgtt aagtgcacgg atgaccgttg
    70741 cggaaggtag attggtaacc gatgtaagac cgggtaattt ccgctctccc acgactttca
    70801 cgatcactta tcagtaatag cggataagga tattagaatg ctatgcaggc atcataaaat
    70861 cgtacacttt ttagggctgg caacagccct tatcacgcct tttgcctatt ccggccagga
    70921 tgtggatctc accgcaaaga tcgtgcccag cacctgtcag gttgaagtca gtaataatgg
    70981 cgttgtcgat ctcggcacag tgacgctgga ctattttgcc gacaatgtaa ccccgacaac
    71041 ggattatgcg ggtggcaaaa cgtttaatgt gaatgtggtc tcttgcgata acattcaaac
    71101 gacacagtcg caaatgaaat tagattttca gccgcaggcg ggatcgcttg cacaggcgaa
    71161 taatcaaatt tttagtaatg aatatgaaca gcaggctacc ggggcgaaaa acgtcggcat
    71221 tgtgattttt tcggcccaac cgaatcagca gacatttaac gtgcgaggaa cggatggttc
    71281 ctcaacagca atttattcgg tagcacctgg caatgctgtt ccctcaacct ggacctttta
    71341 ttcccgtatg cagcgggtga ataatgccct tcctcctgaa tcgggaatgg tgagaagcca
    71401 ggttattgtt aacgtatctt acgaataata aggataatta tgcaaaggaa aggcaataaa
    71461 ctgttgattc agttatgcag tgtgatactg ctatttttta ccacatcctg gtatgcattg
    71521 gcgaatgaat gttatataga gagaaatgct gaaggggatt atcacatgaa gataagctct
    71581 actcagctta gtctggcgtc acaaatggtc gaggttccga cagaaatagc cgaagctaca
    71641 tgggatgtaa atattcaact aagaggcgat gccatagggt gtaaatctct tggggatagt
    71701 aaggcagttc actttcttaa tacagctgac ccaagtttaa tatccacgta caccacaacg
    71761 aatggcgcag cgttattaaa aacaactgtt ccaggcattg tgtattctgt cgagttatta
    71821 tgccttagtt gtggtgccgc agatgaactt gatttatggc tacctgcaca aagtggcgca
    71881 gataacttca taccaagcac ccagacgaaa tgggcctatg agtacagtga tcaaagttgg
    71941 tatttacgtt ttcgcttatt cataactcct gaatttaaac ccaagaatgg tgtttccagc
    72001 ggaacaacga tagcaggaaa gattgcgtca tggtatatag gtaccaatga ccagccgtgg
    72061 atcaactttt acattgacaa tgactcttta aagtttttcg tcgatgaacc gacctgtgca
    72121 acagttgccc tggcacaaga tcagggcaac gtcagtggca atcaggtaac gcttgggaac
    72181 agctatgttt cggaagtgaa aaatgggctt acgcgggaaa tccctttttc tatccgtgct
    72241 gaatactgtt atgccagtaa aattacggtt aagttgaaag cggcaaataa acccagcgat
    72301 gccacactgg tgggtaaaac gactggctcg gcttcaggcg tggctgtaaa agtaaattca
    72361 acttatgaca atagcaaagt attgttaaaa gcagatggta gcaacacggt tgactacaac
    72421 ttcgccgcct ggtcaaacaa cctgctgttt ttacctttta cggcgcagct ggtaccggat
    72481 ggtagcggta atgctgtcgg tgttggaaca ttttcaggta acgcgacctt ctcctttacc
    72541 tacgaataag taaaacgtca ttccctcctc atcgcaccag agttctgctc tggtgcgaac
    72601 atttccccac actaataacc attgcctccc tgttttcggc tgttagaatg cgatcaatct
    72661 gactttagcc atcaggatac gttatgaaac cgaccaccat tgcttcattg cagaaatgta
    72721 agcaggaaaa aaaacgcttc gccaccatca ccgcctacga ctacagcttc gcaaaactgt
    72781 ttgccgagga agggctgaac gtgatgctgg tgggcgactc gctggggatg accgttcagg
    72841 ggcatgaatc tacccttccc gttaccgttg aagatatcgc ctaccacact accgcagtac
    72901 gtcgtggcgc gccaaactgc ctgctgttgg ctgacctgcc gtttatggcg tatgccacgc
    72961 cggaacaagc ctttgaaaac gccgcaacgg ttatgcgtgc cggtgctaac atggtcaaaa
    73021 ttgaaggcgg tgagtggctg gtcgaaaccg taaaaatgct gaccgaacgt gccgttcctg
    73081 tatgtggtca cttaggttta accccacagt cagtgaatat tttcggtggc tacaaagttc
    73141 aggggcgcgg cgatgaagcg agcgatcgac tgcttagcga tgcattagcc ttagaagccg
    73201 ctggtgcaca gttgctggtg ctggaatgcg tgccggttga actggcaaaa cgtattaccg
    73261 aagcgctggc gatcccggtt atcggtattg gcgcaggcaa cgttaccgat gggcagatcc
    73321 tcgtgatgca cgatgcgttc ggcattaccg gcggtcatat ccctaaattt gccaaaaatt
    73381 tcctcgctga aacgggcgac atccgcgctg cggtgcggca gtatatggct gaagtggagt
    73441 ccggcgttta tccgggcgaa gaacacagtt tccattaagg agtgacgttg tgttaattat
    73501 cgaaaccctg ccactgctgc gccagcaaat tcgccgcctg cgtatggaag gcaagcgcgt
    73561 ggcgctggta cctaccatgg gcaacctgca tgatggccac atgaagctgg tcgacgaagc
    73621 caaagcccgc gccgatgtgg tcgtcgtcag tattttcgtt aacccgatgc agttcgaccg
    73681 cccggaggac ctggctcggt acccacgcac cttgcaggaa gactgcgaaa agctgaacaa
    73741 gcgcaaagtg gatctggttt tcgcgccctc ggtaaaagag atctacccga acggtactga
    73801 aacccatact tacgttgacg ttccagggct ttcgaccatg ctggaaggcg ccagccggcc
    73861 ggggcatttt cgcggcgtgt cgactatcgt cagcaagctg tttaacctgg tccagccgga
    73921 catcgcctgc ttcggtgaaa aggactttca gcaactggcg ctgatccgca aaatggttgc
    73981 tgatatgggc tttgatattg agattgtcgg tgtgccaatt atgcgcgcca aagacggtct
    74041 ggcactgagt tcccgtaacg gttatctgac ggcagaacaa cgcaaaattg cgcccggtct
    74101 gtacaaagtt ttaagttcga ttgccgacaa attgcaggct ggcgaacggg atctcgatga
    74161 aattattgct attgccgggc aagaactgaa tgaaaaaggc ttccgcgccg atgatattca
    74221 gattcgcgat gccgacacat tgctggaagt ctctgaaacc agcaaacggg cagtaattct
    74281 ggtagccgcc tggcttggcg atgctcgcct gatcgacaac aaaatggtcg agctggcgta
    74341 atacttatac tctaaataat tcaagttgca agttgcagga aggcggcaag cgagtgacaa
    74401 attcgtcagg aacgaatttg cccagccgca ggctggcctt cggtgagaga catggatgtc
    74461 tctcattaat ccccaggagc ttacataagt aagtgactgg ggttagcgaa cgcagacgca
    74521 gcacatgcaa tttgaagtat gacgagtata actggcgcta cggctgatgg cgccaattat
    74581 taatttgccc cacgtcatgg cgatggcgtc tgtaagcaat tattcataac gcagacaact
    74641 tagttaatca gcctatcaat ctcagcaaga gataaatttg tcatctctgc aacgtcttcc
    74701 cgagacatcc ctttactcag aaaacgctgg gcgaattctt gcctctcctc ctgtcttccc
    74761 tgctgaatcc ccttctcaat ccctttctct tcaaaccact gtgccagcgt catcatagac
    74821 ttccctcccg tttccctgtc tctcagcaca ccataaaaca aatccgcttg ttcagtatga
    74881 ccgcgttgca gcatatagtt ttgcatggca actaactgac tttcgctagt gtacccttcg
    74941 tcaatcagcg tgaccagttg ctccagcaat aacattaagt cgcgctggcg aatatgtttt
    75001 tgcagtagtt cgagaatcgc aatccgccga tgttgcatga tttcgtcatc cggtgtgatg
    75061 gtgatatcca ccagcgggaa aggactgtta tagacgcgtc gcgccagctc cggcgagtaa
    75121 aacatatcaa accagcacat tgagagcgga taaggtgtgg cctcgccctg ataaaagaga
    75181 atcggcacta ccagcggtag tttgtcgtga tccgcctcca gatgccgatg catggcggct
    75241 atagaataac gcatcatgcg aaaggccatt ttcttatccg gcttgctttg gtgttcaatc
    75301 acaacatgca gataaccggg attgccctgc atttgcacgg aatagagcac gtccgtgctg
    75361 tgtcctttaa ggctctcttc aatgaaactc cccgactcta aatgaagcgt gttgaggtca
    75421 caaagttcgc gtaattccac tggcaaatgt atctccagaa agtcgcgagc cgtctccgca
    75481 tgcattaaaa attgtttaaa taccgcgtca tgcggtgtgg tactcggtgc atccatcttc
    75541 cctgtcttcc gtgttgtctt gcgtgaaggc agtatccagt caaggcgtgc tatcgtcagc
    75601 cacctcttta ttgtttaccg agcagcgttc agagaaatat caattcgtta caggcgatac
    75661 atggcacgct tcggcgcggt acgcaaactg ctgatgcaaa tgccgtcaga tgagtagaca
    75721 ctaaacaaaa atcgggcaat actgcgtgag aatttcctca aagcaggcac cgcctgcttc
    75781 gttaacgaca gggtagaaag gtagaagtta tgattcgcac gatgctgcag ggcaaactcc
    75841 accgcgtgaa agtgactcat gcggacctgc actatgaagg ttcttgcgcc attgaccagg
    75901 attttcttga cgcagccggt attctcgaaa acgaagccat tgatatctgg aatgtcacca
    75961 acggcaagcg tttctccact tatgccatcg cggcagaacg cggttcgaga attatttctg
    76021 ttaacggtgc ggcggcccac tgcgccagtg tcggcgatat tgtcatcatc gccagcttcg
    76081 ttaccatgcc agatgaagaa gctcgcacct ggcgacccaa cgtcgcctat tttgaaggcg
    76141 acaatgaaat gaaacgtacc gcgaaagcga ttccggtaca ggttgcttga ttgttatccc
    76201 tgcggctggt tactcaccag ccgcgacatc gtctccagcg aatccgttct taagatataa
    76261 agtcgtttta gtaacaacgg attatccccc ggttttactt tgcctttcat ggttgtcacc
    76321 gccaggtgaa atccggcatc gtttgctgcc ttcacggctt tgtcattaaa gccgccaaac
    76381 ggatacgaaa gataccagac atgcggatta aattgcgcca gagcgcgacg tgaacgtgca
    76441 aaatcaaaca gaatattgtg ctcactacgg ctcagtaata tgggtcggcg ataaccatct
    76501 acccgatgca aaaaatgggt atgtgactgg aaatcaaata catcgcgaat ttcgttaagc
    76561 tcagaaacgc tcataaattg cagcgatttt ggttcccact tctgcgggtg acgtttgatg
    76621 cgcgaggtaa caataaacgc cgtcgccttc atgccatatt gtttcaacac aggatacgca
    76681 tagcggctca ctgacttgag gccatcatca aaggtaatca ccaccgctcg cgcaggaaga
    76741 ttgatcttat tcttcacgta accttccagc tgcaccatgc tcagtgtcgc gtatcccctg
    76801 tcgcgcagcc aggccatctg gttattgaaa gcgcgtaccg aggtggtcgt cgaagtatgg
    76861 cgaaaacggg tgttttcttc gtcgcgcaga atatggtgat aggttagcac cggaaggcca
    76921 ttatcgggtt gggcatccag cgcgctgata taggccagtc gatcgccaat acggatctga
    76981 taccaggttt gatttaacct gtctttcagt ttatgcaaaa tcgggtagcg caaattgtcc
    77041 gccagtaccc caaatggcgc actgcccaca ctcggcgcgt tatagaccgg cgtatctttc
    77101 caggtaacta agttctgatt actcagcggc ttgttgaggt cacccaaacc gtcttcaact
    77161 ttttgtcgcc cctgaaccgg ctcaagatga cctttatcga taaacccttt atcaaagcca
    77221 aaattaaatg cgtaataact tgcggcagtg ggctccacgg caatgatttg tccggcccga
    77281 atattcccca cggtcaccat cttgtcacca atttgcgccc agaccgcagc attttcgatg
    77341 gtttgcatat aacgggcagg taacgcggca ctgacacttg cggtaaacag catcagcagg
    77401 agaataacag cttgtttgta catgaaaaaa tcaccgaggg tgaaacaacg cttttattgt
    77461 agcaaaagcg ccatcagaat cgcgggttaa tactcataca aattatgttt gaggacgaaa
    77521 ggcggatttt tttgttgctg gtgccaaata ctactcacct ccggcgcacc cagttcgaca
    77581 atacgctcgc gaaactctga actggtcatg gtttcacggc aagccatcat ctgttcaatt
    77641 aacggtaacg tgacaccaga aatcacttcg caacgggagt gtttgtggct taataatgaa
    77701 gccacgcggt acggtggcgc tccggctata tcggttaaga agatgacacc ctcaccagag
    77761 tccgcttcat gcagagcatc gcacatcatg cggctgagca tattagaact taatccgcgc
    77821 cagaaattca cggcccggca ctgaagaagc gccccatgtt ttttctccag cacatccagt
    77881 atctcttgcg cccggtcatc gtgacaggta attacccaac ctaacatcgc cttcctcctt
    77941 agccgaaaat cagtctaccc tgaccattgt tgataaaggg tgatagccgt caaaactcct
    78001 ctcccccaaa tccagaggag aggaaatagc cttagctacg caaaccacgt ccacgttgga
    78061 tcagcgacca acagatcaaa taaaacgcca caataaagac caccagtacg ccaaaggtag
    78121 tgaccagcgg aacatcattg ataccgagga agccgtagcg gaaaccactg atcatataaa
    78181 cgattgggtt caggtgcgac agcccttgcc agaacggcgg caacaaagtc agtgagtaaa
    78241 agaccccacc caaatacgtg agtggcgtta acacaaaggt tggcaccagg ctgatgtcat
    78301 cgaacgtttt ggcaaacaca ccgttcagca aacccgcaag ggagaacaac accgccgtga
    78361 gcaccagggt taaggcaacg aatacccacg aatgcacctg aaacggcaca aaaaacagtg
    78421 aaattgccgt caccagaatg ccaacaaaca gaccacgcgc cacgccaccg ccgacatatc
    78481 cggcaataat gacgtgagtc ggaaccggcg ctaccagcag ctcttcaata ttacgctgga
    78541 acttggcacc aaaaaatgat gacgcgacgt tggcgtaggc gttggtgatc accgacatca
    78601 tgatcagccc cggtacgatg aactgcatgt agctgaagcc atgcatatcg ccaatacgcg
    78661 aaccaatcag gttaccgaaa ataataaagt aaagggtcat ggtgatgact ggcggcacca
    78721 gcgtctgcac ccagatacgc ataaagcgat ggatctcttt cgcccagatg ctttttagcg
    78781 ccacccagta aagatgcatc atgcgcgatc tccttgtttt tcattaacca gtgaaacaaa
    78841 cagctcttcc agacggttag ctttgttacg catacttaat acctgaatgc cctgctcact
    78901 taactgcgta aatacgctgt tgatcccctg ctcacgcagc acttcaactt ccagcgtcgc
    78961 ggtatcgacc agtcgatact gatagccatc gagcttcggt aacggacttt tcggtgcgag
    79021 atcgagaata aaggtttccg atttcagctt cgccagcagc gccttcatcg aggtattttc
    79081 caccagctca ccgtgttgaa taatgccgat attgcggcac agcatttctg cttcttccag
    79141 gtagtgtgtg gtgagaatga tggtggtgcc tttgtcgttt aaatccttca aaaagcccca
    79201 cattgagcgg cgaagttcaa tatccacgcc tgcagtcggc tcgtcgagaa tcagtagttt
    79261 aggttcatgc attaacgcac gggcaatcat taaacggcgc ttcatcccgc cagataacat
    79321 gcgcgcacgt tcgttgcgtt ttccccataa atcgagctgc ttaagatatt tttcgctgcg
    79381 gatgtacgcc tctttgcgct ccacaccgta gtaccctgcc tgattcacca caatttgctg
    79441 cacggtttca aacgggttga agttaaattc ctgcggcacc agtcccaact gacgtttagc
    79501 gttcacgaca tctttctcga gatcgtaacc aaatacgctg acccgcccgg aggttttatt
    79561 taccagagag ctgataatac cgatagtggt cgatttcccg gccccgttcg gcccgagaag
    79621 cgcataaaaa tcacccgctt cgacctgcaa atctattcca cgaagcgcct gaacgccgcc
    79681 tggatagttt tttttaagct gttgaagttc cagtgcaatg gtcataaatt tttacttacc
    79741 ttacgttctt acactttatc tgtggtttaa atcgtcccgg agttgcccta tattagccaa
    79801 acgtaattat ttggttacag gtcgttaacc tccatgaaag acatagatac actcatcagc
    79861 aacaatgcac tatggtcaaa aatgctggtg gaagaggatc ccgggttttt tgagaaactg
    79921 gcacaagcgc aaaaaccgcg ctttctatgg attggatgtt ccgacagtcg cgttcctgca
    79981 gaacgtttaa ccggtcttga gccgggcgaa ctctttgttc accgtaatgt tgctaacctg
    80041 gtcattcaca ccgacctgaa ctgcctttcc gtggttcagt atgcagtgga tgtactcgaa
    80101 gttgaacaca ttattatctg tggccactac ggttgcggcg gcgtacaagc cgcagttgaa
    80161 aacccggaac tggggcttat caacaactgg ctgctgcata tccgcgatat ctggttcaaa
    80221 catagctcat tgctcgacga aatgccgcaa gagcgccgtc tggatacctt gtgtgaactg
    80281 aacgtcatgg aacaggtgta taacctgggc cactccacca ttatgcaatc agcgtggaaa
    80341 cgcgggcaga aagttaccat tcacggctgg gcttatggca ttcacgacgg cttgctgcgt
    80401 gatctggatg ttactgccac caaccgcgaa acccttgagc aacgttaccg tcacgggatt
    80461 tccaacctca agctgaaaca cgccaaccac aaataaaaat gccatgccgg atgcaacaca
    80521 tccggcaact tcacacttac tcgtccagca gaatcacttt gccgatatac ggcagatgac
    80581 ggtaacgctg tgcgtaatca atgccgtaac ccaccacaaa ctcatccggg atcgagaaac
    80641 cgataaattc taccgggacg ttcacttcac gacgggacgg tttatccagc agcgtacaaa
    80701 tcgccaacga cttcggttcg cgcaggctta agatctcacg cactttcgac agtgtattcc
    80761 ccgagtcgat gatatcttca acaatcagca catccttgcc acggatatct tcatccagat
    80821 ctttgaggat tttcacatca cgggtggtgg acatgccgct accgtagctg gaggcggtca
    80881 taaagtcgac ttcatgagat acctgaactt cacggcacag gtccgccata aacataaatg
    80941 agccacgcag cagacccacc agcaccatat cgctgccgct gtctttgtaa cgctcagtaa
    81001 tctgacgacc cagttcggcg atacgcgctt taatctccgc ttcggggatc attacttcta
    81061 cagtatgttt catatctcta accatatgat ttaaaaacaa atcacttcgc acgggcaggt
    81121 tttggcaccg caacaaagcg caagacacgc agtataccag caaaacgatt cattcgcgga
    81181 atctgttaat aaagctcaaa ttgtgatgac gatcacacat gttaaaacct ataattaatt
    81241 gctactaaaa tattaatgaa ttgaaatggt gtctctttat ggcaattaac aatacaggct
    81301 cgcgacgatt actcgtcacg ctaacagccc tttttgcagc gctttgcggg ctgtatctac
    81361 tcattggcgg aggctggctg gtcgcgattg gcggctcctg gtactaccct atcgctggcc
    81421 ttgtgatgct cggcgtcgcc tggatgctgt ggcgcagtaa acgtgccgcg ctttggctat
    81481 acgccgccct gctgctcggc accatgattt ggggcgtctg ggaagttggt ttcgacttct
    81541 gggcgctgac tccgcgcagc gatattctgg tcttcttcgg catctggctg atcctgccgt
    81601 ttgtctggcg tcgcctggtc attcctgcca gcggcgcagt tgccgcactg gtggtggcac
    81661 tgctgattag cggtagtatc ctgacctggg ccggatttaa cgatccgcag gaaatcaacg
    81721 gcaccttaag cgccgatgcc acacctgcgg aagctatctc acccgttgcc gatcaggact
    81781 ggcctgccta tggtcgcaat caggaaggtc aacgcttttc acctctgaaa caaattaacg
    81841 ccgataacgt ccataatctg aaagaagcct gggtgttccg tactggcgat gtgaagcagc
    81901 cgaacgatcc gggcgaaatc accaatgaag tgacgccgat taaagtgggc gacacccttt
    81961 acctgtgtac cgctcaccag cgcctgtttg cgctcgatgc cgccagcggc aaagagaaat
    82021 ggcattacga tcctgagctg aaaactaacg agtctttcca gcacgtaacc tgccgtggcg
    82081 tctcttatca tgaagccaaa gcagaaacgg cttcgccgga agtgatggcg gattgcccgc
    82141 gtcgtatcat tcttccggtc aatgatggtc gcctgattgc gattaacgct gaaaacggca
    82201 agctgtgcga aaccttcgcc aataaaggcg tgctcaatct gcaaagcaat atgccagaca
    82261 ccaaaccggg tctgtatgaa ccgacttcgc caccgattat caccgataaa accatcgtga
    82321 tggcaggttc agttaccgat aacttctcaa cccgcgaaac gtctggcgtg atccgtggtt
    82381 ttgatgtcaa caccggggag ctgctgtggg cttttgatcc gggcgcgaaa gatccgaacg
    82441 caatcccgtc tgacgaacac acctttacct ttaactcgcc aaactcgtgg gcaccagcgg
    82501 cctatgacgc gaagctggat ctggtgtatc tgccgatggg cgtgaccacg ccggatatct
    82561 ggggcggtaa ccgcacaccg gaacaggaac gttatgccag ctcgattctg gcgctgaatg
    82621 ccacaaccgg gaaattggcg tggagctacc agaccgttca ccacgacctg tgggatatgg
    82681 atcttccggc acagccgacg ctggcggaca tcaccgttaa tggtcagaaa gtgccagtta
    82741 tttacgctcc ggcgaaaacc ggcaacattt ttgtgctcga tcgtcgtaat ggcgaactgg
    82801 tggttccggc accggaaaaa ccggttcccc aaggtgctgc caaaggcgat tacgttaccc
    82861 ctactcaacc attctcggaa ctgagcttcc gtccgacgaa agatttgagc ggtgcggata
    82921 tgtggggagc caccatgttt gaccaactgg tgtgccgcgt gatgttccac cagatgcgct
    82981 atgaaggcat tttcacgccg ccatctgaac agggcacgct ggtcttcccg ggtaacctgg
    83041 gaatgtttga atggggcggg atttccgttg atccaaatcg tgaagtggca attgccaacc
    83101 caatggcact gccgtttgtt tcgaaactga tcccacgcgg tccgggtaac ccgatggagc
    83161 agccgaaaga tgccaaaggc acgggtacgg aatccggcat tcagccacag tacggtgtac
    83221 cgtatggtgt cacgctcaac ccgttcctct caccatttgg tctgccatgt aaacagccag
    83281 catggggtta tatctcggcg ctggatctga aaactaatga agtggtgtgg aagaaacgta
    83341 ttggtacgcc gcaggacagt atgccgttcc cgatgcctgt accggtgccg ttcaatatgg
    83401 gtatgccgat gctgggcggg ccaatctcca cggcgggtaa cgtgctgttt atcgccgcta
    83461 cggcagataa ctacctgcgc gcttacaaca tgagcaacgg tgaaaaactg tggcagggtc
    83521 gtttaccagc gggtggtcag gcgacgccaa tgacctatga agtgaatggt aagcagtatg
    83581 tggtgatctc cgcaggcggt cacggttcat ttggtacgaa gatgggtgac tatattgtgg
    83641 cttatgcgct gccggatgat gtgaagtaag acttgctcag attgctgaca acgtgcgcgt
    83701 tgttcatgcc ggatgcggcg tgaacgcctt atccagccta caaaattgtg caaaatcaat
    83761 ggattgcaca ggtaacgtag gcctgataag cgtagcgcat caggcagatt tgcgtttgtc
    83821 atcagtttaa tgcccggaga gatccgggca tattcccgaa tacggtcttt ttataccgta
    83881 aaccctaaca tcatccccgt atcttcatgc tccagcagat ggcagtgcgc catataagca
    83941 cgttctttcg gtgcgtcgtg attaaacttc accagcactt cactgacatt gccttctacc
    84001 ttaacggtat ctttccagcc cgcgcgatga gccgctggcg gtttgccatt ttctgacaag
    84061 atacggaact gcgtgccgtg aatatggaac ggatgcagca tcatgtcgcc cacgccagag
    84121 ataacccaac gttcgtactg ccctttcgcc gccgcaaaca tcggcttatt catatcaaac
    84181 gcctgaccgt tgattttatt ggcatggtgg aaatcgaact tcccgccgtg gttcatatga
    84241 ttcatattgc cgtgccccat atggcccatc atctggctgt gatccattcc ggccatcgcc
    84301 tgatcgccat atttctccat tagcatctgc atccccatca tatcgagcat cgggtccata
    84361 gaaagttgca gcttgcgtac cgtcagccct tccagcgaag gtaacgcagg caggctactt
    84421 aatgtgtctg gcaaagcacc ggaagcacta atagcaatcg gctgaatccg cattaccgga
    84481 tgaggcttat caaacggcgc aatcgccatc cccatctggc tgaccggcag cgtcaccagg
    84541 tcaaagggtt tgttgtcgtt aacctccacc agcacttcaa aacgctcgcc catcagcacc
    84601 ggcagctcgt tcaccttcac cggttcaggt agcagaccac cgtcgctggc aatcacataa
    84661 agcgggcgat tgtcgctggt ggcgaaattg agcgagcggg cattacagcc attgagcaaa
    84721 cgcaggcgca gccaaccacg cggggcagcg tgttgcgggt agattgcacc gttggtcagc
    84781 aacgtatcgc caaaccagcc cacggcggcg gtcatcacat ccagttgata atcaatctgc
    84841 ccgtcggcgc taaatttctt atcctgaacg atcaccggaa catcatcgat accccactgt
    84901 tttggcagca ttaatttcag gatctcgtca tcttcaatca ccaccagacc agccagcccc
    84961 atcgccacct gtcgcccggt cttgccatgt tgatgtggat ggaaccagca ggtagcggca
    85021 ggttgatcaa cgttcaacgt caccgagcgc ttgccacctg gcggaataat tccctgtggg
    85081 ccgccgtcga cttcacccgg tacttccagc ccgtgccagt gcaacgtcgt ctcttccgtc
    85141 agttggttat agatatcaac cgttaccgct ttgccgcgct gtaatttcac cgccggcccc
    85201 agcagattgc cgttatagcc ccaggtagtt gcagttttct cgccaaaggt agactgacct
    85261 gcgccaatag ttaactgaat gcgattacgg gcatcggtcg tgagcaaatc agggatcggt
    85321 aacgttgggc gttccgccgc aaagactgca cggctccaca gtggcaaggc tgaagccaca
    85381 cccagtgcga cggaatattt caagaaatca cgacgttgca tagttatttc cttattctta
    85441 agcaggcgaa acaaaatcaa acgttgagca tagtccttcc ccttacggga aggtcaagcc
    85501 gcagacacaa tttaatcgac aataatggtc gtcgcctcgc tcgcagtgtg ctaacgttta
    85561 tcttctttaa gcccctggta gaagcaatga agacgttttt cagaacagtg ttattcggca
    85621 gcctgatggc cgtttgcgca aacagttacg cgctcagtga gtctgaagcc gaagatatgg
    85681 ccgatttaac ggcagttttt gtctttctga agaacgattg tggttaccag aacttaccta
    85741 acgggcaaat tcgtcgcgca ctggtctttt tcgcccagca aaaccagtgg gatctcagta
    85801 attacgacac cttcgacatg aaagctctcg gtgaagacag ctaccgcgat ctcagcggca
    85861 ttggcattcc cgtcgctaaa aagtgcaagg ccctggctcg cgattcctta agcctgcttg
    85921 cctacgtcaa ataatccctg ataccttttt gtagaaatat tgaccgtgca tccgcggtca
    85981 atgttagcta ttatgttgcg cccttttttt acgggtgtta acaaaggagg tatcaaccca
    86041 tggccgaaaa aaaacagtgg catgaaacgc tacacgacca gtttgggcag tactttgcgg
    86101 tagataacgt tctgtatcat gaaaagaccg atcaccagga tctgatcatt tttgagaacg
    86161 ctgcatttgg tcgcgtaatg gcgctggatg gcgtagtaca aaccaccgag cgcgacgagt
    86221 ttatctatca tgagatgatg acccatgttc cgctactggc ccacggtcac gcgaaacatg
    86281 tgctgattat cggcggcggc gacggtgcca tgctgcgtga agtaacccga cataaaaacg
    86341 ttgagtcaat cacgatggtg gaaatcgatg cgggtgtcgt gtcgttctgc cgtcagtatc
    86401 tacccaacca taacgccggt agctacgacg atccgcgctt taagctggtg atcgacgatg
    86461 gcgtcaattt cgttaatcaa accagccaga cctttgatgt cattatctcc gactgcaccg
    86521 atcctatcgg tcccggcgaa agccttttca cttcggcatt ttatgaaggc tgcaaacgtt
    86581 gcctgaatcc tggcggtatc ttcgtcgcac aaaacggcgt ctgcttttta cagcaggaag
    86641 aagccatcga cagccatcgc aaactcagcc attacttcag cgacgttggc ttttatcagg
    86701 cggcgatccc gacctattac ggcggtatca tgacttttgc atgggcgaca gataacgacg
    86761 ccttacgcca tctctcaacc gaaattattc aggcgcgttt tctcgcctct ggcctgaaat
    86821 gccgttatta caatccggca gtccatacgg cagcttttgc cttaccccag tatctgcaag
    86881 acgcactggc ttcacagccg tcctaaggag aagataagaa attgaaaaaa ctgaaactgc
    86941 atggctttaa taatctgacc aaaagtctga gtttttgtat ttacgatatc tgctacgcca
    87001 aaactgccga agagcgcgac ggttatattg cttatatcga tgaactctat aatgccaacc
    87061 gtctgaccga aatcctgtca gaaacctgtt ccattatcgg ggctaatatt cttaacatcg
    87121 cccgccagga ttacgaacca cagggtgcca gcgtcactat tctggtgagt gaagaaccgg
    87181 ttgacccgaa actcatcgac aaaacagaac accccggccc actgccagaa acggtcgttg
    87241 cccatctcga taaaagtcat atttgcgtac atacctaccc ggaaagtcat cctgaaggcg
    87301 gtttatgtac cttccgcgcc gatattgaag tctctacctg cggcgtgatt tctccgctga
    87361 aggcgctgaa ttacctgatc caccagcttg agtccgatat cgtaaccatt gattatcgcg
    87421 tgcgcggttt tacccgcgac attaacggta tgaagcactt tatcgaccat gagattaatt
    87481 cgattcagaa ctttatgtct gacgatatga aggcgctgta tgacatggtg gatgtaaacg
    87541 tctatcagga aaatatcttc cataccaaga tgttgcttaa agagttcgac cttaagcact
    87601 acatgttcca caccaaaccg gaagatttaa ccgacagcga gcgccaggaa attaccgctg
    87661 cgctgtggaa agaaatgcgc gagatttatt acgggcgcaa tatgccagct gtttaacggc
    87721 tctggcggag ctcccaggct ccgccagatt tatttacttc tgctgcacga aattgcggta
    87781 agccgccacg acctgcagaa aatcctcaac gccgcatagc gacaggcttt cttcgtcgta
    87841 gtagttcatc ccctcttcca tttcatcgcc agcgaattcc agttgattgg cgcgaaccat
    87901 cacctcttcg ccgtccatcc atagcgtata ctcatgccct gcccgctgcc aggaacgttc
    87961 gctacctttc agtgcgtgcg ctgcttgttc cacttcatca agcaaggcca ggttttcttt
    88021 cacctcttca ttaaaccagt gcccgaccac ttcatgcccc atggacatac gcacctttac
    88081 cactccggta atatcgcgca gaaattcgta atccatagtg tgttcctctg ctcccggcaa
    88141 tgcaccgaaa tcgcattgcg ccattgatgt aattatcgca gccttaccgg agaaaaacag
    88201 cggaacggcg atggtttaga aataaaaaaa tgcccgggaa tctcattcac cgggcattgt
    88261 gtcgtttatg cgcagcgcgt gcgctgactt tttaaaccgc agtctggaaa atcaccccat
    88321 cggctttctc ggtgtactga gaaagctggt tgaagttcag ataacggtaa gtatcaacgg
    88381 ctgttttatc tacctgcgcc acgtaggtct ggtactcttc cggcgtcggc agtttgccaa
    88441 tcagcgccgc aacagcagcc agttccgcag aagccaggaa gacattcgcg ccagtaccca
    88501 gacggttcgg gaagttacgg gtagaggtgg aaaccaccgt tgcaccgtcc gccacacgcg
    88561 cctggttacc catacacagg gaacagccag ggatctcgat acgcgcacca ctcttaccga
    88621 agacgctgta gtagccttct tcggtcaact gtgcggcgtc catacgggtt ggcggtgcca
    88681 cccacaggcg ggtcggcaac tgacctttgt gcgcatccag cagtttacct gcggcacgga
    88741 agtgaccgat gttagtcatg caagaaccga taaacacttc gtcgatcttc tcgccctgta
    88801 ccgcagacag cggacgtgcg tcgtccgggt cgttcggagc acagaggatt ggctctttaa
    88861 tatccgccag atcgatgtcg atcactgccg cgtattccgc atctgcatcg gcttccagca
    88921 gctcaggatt cgccagccat ttttccatgc cctgaatacg acgttccagg gtacgacgat
    88981 cgccgtaacc ttccgcgatc atccacttca gcaggacgat gttggagttc aggtattcga
    89041 tgattggttc tttgttcagc ttgatggtac aaccggcagc agaacgctcg gcggacgcat
    89101 cggtcagctc aaaggcctgc tcaactttca gatccggcag accttcaatt tctaggatgc
    89161 ggccagagaa gatgtttttc ttgcctttct tctcaacggt cagcagacct tgtttgatcg
    89221 catacagcgg aatggcgtgt accagatcgc gcagggtgat gcccggctgc attttgcctt
    89281 tgaagcgcac cagaacggat tccggcatat ccagcggcat tacgcctgtt gcggctgcaa
    89341 atgccaccag accagaaccc gccgggaaag agataccgat cgggaaacgg gtatgggagt
    89401 caccaccggt accgacggta tccggcagca gcatacggtt cagccaggag tggattacgc
    89461 cgtcacccgg acgcagcgac acaccgccac ggttcataat gaagtccggc agcgtgtggt
    89521 gcgtgttcac gtcaactggc ttcggatacg ccgcggtgtg gcagaaagac tgcatcacca
    89581 ggtcagccga gaagcccagg cacgccaggt ctttcagttc atcacgggtc atcgggcctg
    89641 tggtgtcctg agagcctaca gaagtcattt tcggttcaca gtacgcgccc ggacgaatgc
    89701 ctttcacgcc acaggcacgg cctaccattt tctgcgccag cgagaagccg cgatcgctct
    89761 cagcgacatc tttcgcctga cggaacacat cactgtgcgg cagaccaagt gcttcacgcg
    89821 ctttggtggt caggccacga ccgataatca gcggaatacg gccgccagca cgcacttcat
    89881 caatcagcac gtcggttttc agttcgaagg tcgccagcag ttcgccggtt tcgtggttac
    89941 gtacttcacc tttgtacggg taaacgtcaa ttacgtcgcc catgttcagg ttagagacat
    90001 ccacttcgat tggcagcgca cccgcatctt ccatcgtgtt aaagaagatg ggtgcaattt
    90061 taccgccgag gcacaaacca ccgccgcgtt tgttcggcac atgtggaata tcatcgccca
    90121 taaaccacag aacggagttc gtggcggatt tacgcgatga acccgtaccc acaacgtcac
    90181 cgacgtacgc cagcgggaaa cctttctgtt gcagagcttc aatctgtttg atcgggccaa
    90241 caacacctgg ctggtctggc tcaatacctt cacgggcgtt tttcagcatc gccagcgcgt
    90301 gcaatgggat atccgggcgt gaccacgcat ccggtgccgg agaaaggtca tcggtgttgg
    90361 tttcgccagt gactttgaag acggtaacgg tcagtttttc agccagcgcc gggcgattca
    90421 ggaaccattc ggcatcagcc caggactgca taacctgctt cgcatattcg ttgcctgctt
    90481 tcgctttctc ttctacgtca tagaagttat cgaacatcag tagtgtgtga gaaagtgctt
    90541 tggcagcgat cggtgccagt ttggcatcat ccagcgcatc gatcagcgga tgaatgttgt
    90601 aaccaccctg catagtaccc agcagttcga tggctttttc cggagtcagc agtggggatt
    90661 tggcttcgcc tttggcgaca gcagccagga agcctgcttt aacataggcg gcttcatcga
    90721 cgccaggagg aacacggttg gttaacagat ctaacaggaa ttcttcttcg cccgcgggcg
    90781 ggtttttcag cagctctaca agcgcggcca tttggtttgc atccaggggt ttgggcgcaa
    90841 tcccctcagc ggcacgctca gctacgtgct tacggtattc ttctagcacg acggttctcc
    90901 tcgctctcat tgtcatagtg cggcaggcga ttctcttcac gctcctgtga gacagcagtt
    90961 tgtagggtaa atgcccagta ccgcaacggg cagaatagca ggattttgac gacgtgttaa
    91021 tctgtttaca aaaaagcaac ataaaaaagt ggctgaatcg ttaaggatgg tctagagatt
    91081 gtttccctcc gtaaaaactt cgttaattcg catggtgata atcacctttc tcaacgaatc
    91141 ccaagattag tcaaaattta aactaccgcc tctttatact cggattcaca gcacctgcgg
    91201 gtggcagttc gccgaccatt gcgatttcct tgagatccga attatgaaaa tgactttgcc
    91261 gtttaaaccc catgtgctgg cgctaatttg cagtgccggg ctttgtgccg cctctgccgg
    91321 gctatatata aaaagccgca cagtggaagc gcctgtggaa acgcaatcga cacaactggc
    91381 tgtgtctgac gctgccgcag ttacgcttcc tgcaacggtt tccgcacctc ccgtaacacc
    91441 cgccgtcgtc aaatccgcat tcagcactgc acaaatagat caatgggtcg cgcccgtcgc
    91501 gctgtatccc gacgccctac tttcgcaggt gctgatggca tcaacctatc cgacaaacgt
    91561 tgctcaagca gtgcaatggt cgcacgataa tccacttaaa caaggcgatg ctgctattca
    91621 ggcggtatct gaccagccgt gggacgccag cgttaaatca ctggtggcct ttccacaatt
    91681 gatggcattg atgggcgaaa acccgcaatg ggtgcaaaac ctgggcgatg cttttctggc
    91741 ccagccgcag gacgtgatgg actcggtaca acgattgcgg caactggcac aacaaaccgg
    91801 ctcgctgaag tcatcaaccg aacagaaagt tattaccaca acgaagaaaa ctgtaccggt
    91861 aacacagaca gtcacggctc ccgtcatacc atccaatacc gtttcaactg ccaaccctgt
    91921 cattacagag cctgcaacaa ccgtcatttc cattgagccc ggcaatcctg atgtggtcta
    91981 tattcccaac tacaacccaa ccgtggttta cgggaactgg gccaatactg cgtatccgcc
    92041 ggtttatctg ccaccaccag ccggagaacc gtttgttgac agctttgtac gcggattcgg
    92101 ctatagcatg ggcgttgcta ccacgtacgc actattcagc agcatcgact gggatgacga
    92161 cgatcatgac catcatcatc atgacgatga taattatcat caccacgatg gcggtcatcg
    92221 tgacggtaat ggctggcaac ataacggcga caacatcaat atcgacgtca acaatttcaa
    92281 ccgtatcacc ggtgagcatc ttactgataa gaatatggca tggcggcaca atccaaacta
    92341 ccgtaatggt gtgccctatc atgatcagga tatggcaaag cggtttcatc aaactgatgt
    92401 caacggcgga atgagtgcca cgcagctacc tgctccaaca cgcgacagcc agcgtcaggc
    92461 ggcagcaagt cagtttcagc aacgaacaca cgccgccccc gtcattacac gagataccca
    92521 acgtcaggca gcggcacagc ggtttaatga agctgaacac tatgggagct atgacgactt
    92581 ccgcgacttc agccgtcgcc aaccactgac ccagcaacaa aaggacgccg ctcgtcagcg
    92641 ttatcagtca gcttctcctg agcagcgcca ggcagttcac gagaaaatgc agactaaccc
    92701 gcagaaccag cagcgaagag aggcagcgcg tgagcgcatt cagcccgcct cgcctgagca
    92761 gcgccaggca gtccgcgaga aaatgcagac taacccacag atccagcagc gaagagacgc
    92821 agcgcgtgag cgtattcagt cagcctcgcc tgagcagcgc caggtgttta aggaaaaagt
    92881 acagcagcgc ccactgaacc aacagcaacg tgataacgcc cgccagcgtg ttcaatcagc
    92941 atcacctgaa caacgtcagg tttttcggga gaaagttcag gagagccgcc cacaacgtct
    93001 aaacgacagt aaccatactg ccaggctgaa taacgagcaa cggtcagcag tacgcgaacg
    93061 tctctctgag cgcggagcaa ggcgactgga aaggtaaatt acaggcgtaa aaaaagcggc
    93121 gtggttagcc gcttttttaa ttgccggatg ttccggcaaa cgaaaaatta cttcttcttc
    93181 gctttcgggt tcggcaggtc agtaatgcta ccttcgaaca cttctgccgc caggcccaca
    93241 gactcgtgca gagtcgggtg cgcgtggatg gtcagtgcga tgtcttcagc atcacaaccc
    93301 atttcaattg ccaggccgat ttcacccagc agctcgccgc cgttagtacc gacaatcgca
    93361 ccaccgatca cacggtgaga ttctttgtcg aaaatcagct tggtcatacc gtctgcgcag
    93421 tcggaagcga tagcacgacc agaagcagcc cacgggaagg tggcggtttc atagctgatg
    93481 cctttctctt tcgcttcttt ctcagtcaga cctacccatg caacttctgg ttcggtatag
    93541 gcgatggacg ggataacttt cggatcgaag tagtgtttct taccggcgat aacttcagcg
    93601 gcaacgtgac cttcgtgaac acctttgtgt gccagcatcg gctgaccgac gatatcgccg
    93661 atagcaaaga tgtgcggtac gttggtacgc agctgtttgt caacgcggat gaaaccacgg
    93721 tcgtcaactt ccacgccagc tttgcctgcg tcgaggtttt taccgttcgg cacacgacca
    93781 atcgctacca gcacggcgtc gtaacgctgc ggttcagctg gtgctttttt gccttccatc
    93841 gtcacataaa taccgtcttc tttcgcttca acggcggtaa ctttggtttc cagcatcagg
    93901 ttgaatttct tgctgatacg cttggtgaag actttaacga tgtctttgtc agctgccggg
    93961 ataacctggt cgaacatttc aaccacgtca atctgtgaac ccagcgcgtg gtaaacggtg
    94021 cccatttcca gaccgatgat accgccaccc attaccagca ggcgttctgg tacttctttc
    94081 agttccagcg cgtcagtaga gtcccagata cgcggatctt catgcggaat aaacggcagt
    94141 tgaatcgggc gagaacccgc tgcaatgatc gcgttgtcga agttgatcac ggttttaccg
    94201 ttctcacctt caacttccag ggtgttagcc ccggtaaatt tacccagacc gttgaccact
    94261 ttgactttgc ggcctttcgc cataccagcc agaccaccag tcagctgatt gatcactttc
    94321 tctttccagg tacgaatctt gtcgatatcg gttttcggtt cgccgaagac gataccgtgt
    94381 tcagccagcg ctttggcttc ttcgataact tttgctacgt gcagcagtgc tttagaaggg
    94441 atacagccga cgttcaggca aacaccgcca agggtgttgt aacgttctac gattacggtt
    94501 tccagaccta aatcagcgca acggaaggca gcggagtaac ctgcggggcc tgccccaagt
    94561 accacgacct gagttttgat ttcagtactc atcatgacct ctatatattt atctccggcg
    94621 ggtctgacgt tttttttatg acgcccatta tccaccgggt cgttctatcc gtcggtattt
    94681 tacaaaattg ttaacaattt ttaaacaaca aacggcaacc gatttgtcta ttcgctaata
    94741 acctcaatac attcatgaga ttaccagaaa aaagccggcc gttgggccgg ctcttttact
    94801 tacatcacca gacggcgaat gtcagacagc gtgttgttaa tgatggtaat gaaacgggca
    94861 ccatcagcac cgtcgatcac gcggtggtcg aaggagagag aaatcggcag catcagacgc
    94921 ggcacgaact ctttaccatt ccacaccggc tccatcgcgg acttggaaac gccgaggata
    94981 gccacttccg gcgcgttcac aatcggcgcg aagtgggtag tacccaggcc gccgatgctg
    95041 gagatggtga agcaaccgcc ctgcatttcg cccgcagtca gcttaccgtc acgcgctttc
    95101 ttagaaatag tcatcagctc gcgagacagt tcgatgatgc ctttcttgtt gacgtctttg
    95161 aataccggaa caaccagacc gttcggggta tccaccgcca caccgatgtt gatgtatttc
    95221 ttcagggtca gacgctgacc gtcttccgac agcgaactgt tgaagcgagg catctgctca
    95281 agcgctgcag caacggcttt catgatgaag acaaccgggg tgatcttcac atccagctta
    95341 cgtttcgccg cttcttcgtt ctgctgttta cggaacgctt ccagctcggt gatatcggtt
    95401 ttgtcgaagt gagtaacatg cgggatcatc acccagttac ggctcaggtt cgcaccagaa
    95461 attttctgga tacggcccag ttccacttct tcgatttcac caaacttgct gaagtccacc
    95521 ttcggccacg gcagcatgcc agggatacca ccgccagtcg ctgccggagc tgcttctgca
    95581 cgtttgatag cttctttcac gtaagcctga acgtcttcgc gcaggatacg acctttacgg
    95641 ccagtgccct tcactttcgc aaggttaacg ccaaactcgc gtgccagacg gcggatcagc
    95701 ggggtcgcgt gaacgtaagc gtcattttca gcaaattcag atttgccttc cgctttcgca
    95761 gccggtgctg ctgccggggc ttcagctttt gctgccggag ccggcgctgc tgcttcctgt
    95821 ttcgcaggag ctgccgcagg cgctgcgcct tcaacttcga agatcataat cagcgagcca
    95881 gttttcactt tatcgccaac gttgactttc agttccttca cgacgcctgc aaacggagcc
    95941 ggaacttcca tagaagcttt atcgccttct acggtaatca gtgactgttc agcggcaact
    96001 ttgtcgccca ctttcaccat cacttcggtc acttcaactt cgtcaccgcc gatatccgga
    96061 acgttaactt ctttcacgcc agccgctggt gcaggggccg ctgccggagc cgcttcctgt
    96121 ttagccgccg gagctgccgc gcctgcttca cccgccactt cgaagaccat aatcagcgag
    96181 ccggtagaca ctttgtcgcc ggtgttcact ttgatttctt tcacggtgcc tgcaaacgga
    96241 gccggaactt ccatagaagc tttatcgcct tctacggtga tcagcgactg ctcagcttca
    96301 actttgtcgc caactttcac caggatttcg gtcacttcaa cttcgtcgct gccgatatcc
    96361 ggaacgttaa cgtcttttgc cgcggcagcc gcggggactg ctgccggagc tgcttctttc
    96421 ttctcttctg cctgagcagg tgcagcgtct gctgcaccgt cggcggaatc gaaaatcata
    96481 atcagtgcgc cggtctgagt tttatcgcca acagagactt tgatctcttt aacgataccc
    96541 gcctgcggag acggaacttc catagaagct ttgtcgcctt ctacggtgat cagcgactgt
    96601 tcggcttcaa ctttgtcgcc cactttgacc aggatctcgg tgatttcaac ttcatcagcc
    96661 ccgatgtccg gtactttgat ttcgatagcc attattcttt tacctcttac gccagacgcg
    96721 ggttaacttt atctgcatcg atgttgaatt tggcgattgc gtcagcaacc actttcttat
    96781 cgatttcgcc acgtttagcc agttcgccca gcgccgcaac cacgacataa gaagcatcaa
    96841 cttcgaagtg gtgacgcagg ttctcacggc tgtcggaacg accgaagcca tcagtaccca
    96901 gtacgcggta gtcgtcagcc ggtacgtaag tacggacctg ctcagcgaac agtttcatat
    96961 agtcggtaga tgccacagcc ggagcgtcgt tcatcacctg agcgatatac ggaacgcgcg
    97021 gagtttccag cggatgcagc atgttccagc gttcacaatc ctgaccatca cgcgccagtt
    97081 cggtgaagga ggtcacgcta taaacgtcag aacctacgcc gtaatctttc gccaggatct
    97141 ctgccgcttc acggacgtga cgcaggatag aaccggagcc cagcagctga actttacctt
    97201 tgctaccttc aatggtttcg agtttgtaga tacctttacg gataccttcc tcagcacctt
    97261 ccggcattgc cggcatgtgg tagttttcgt tcagcgtggt gatgtagtag taaacgttct
    97321 cttgtttttc accgtacata cgctccagac cgtcatgcat aatgacagca acttcgtaag
    97381 cgtaagccgg gtcgtaagag atacagttcg gaatagtcag cgactgaatg tggctgtgac
    97441 catcttcgtg ctgcagacct tcaccgttca gggtggtacg accggaagta ccgccgatca
    97501 ggaagccacg cgcttgctgg tcgccagccg cccagcacag atcgccgata cgctggaagc
    97561 cgaacatcga gtaatagatg tagaacggaa tcatcggcag attgttggtg ctgtaagagg
    97621 tcgccgctgc cagccaggaa caacctgcgc ccagctcgtt gatcccttcc tgcagaatct
    97681 gacctttctc gtcttcttta tagtaagcaa cctgctcgcg gtcctgcggg gtgtactgct
    97741 gaccgttcgg gctgtaaata ccaatctgac ggaacagacc ttccataccg aaagtacgcg
    97801 cttcgtcggc gatgatcggt accagacgat ctttgatcga cttgttcttc agcatcacgt
    97861 tcagggcacg aacgaaagcg atagtggtag agatctcttt gctctgctct tccagcagcg
    97921 cgccgaagtc ttgcaggctt ggcagctcaa gcttctcggt gaagttcggc tgacggcttg
    97981 gcagataacc gtgcagtttc tgacgctgtg cgtgcagata ggtatgctct tcagaacctt
    98041 ccgggaaggt gatgtacggc agtttttcga tatctgcatc agacaccggc acattgaaac
    98101 ggtcgcggat gtgacgcaca ccgtccatgt tcattttctt aacctggtgc gcgatgtttt
    98161 taccttcagc cgcgtcgccc atgccgtaac ctttaatggt atgagcaagg attaccgtcg
    98221 ctttgccttt ggtttcctgc gctttcttga atgcagcgta gattttcttc ggatcgtgac
    98281 caccacggtt cagtgcccag atctgctcgt cagtccagtc tgcaaccagt gctgcggttt
    98341 caggatattt accgaagaag tgttcacgaa cgtacgcacc atctttcgat ttgaaggtct
    98401 ggtagtcgcc gtcaacggtt tcgttcatca gctggatcag tttaccgctg gtatctttac
    98461 gcagcagttc atcccaacgg ctaccccaca tcactttgat cacgttccag ccagcacctt
    98521 cgaagatacc ttccagttcg ttgatgatct tgccgttacc ggtgaccggg ccgtcaagac
    98581 gctgcaggtt acagttgata acgaagacca ggttatccag tttttcacgg gtagcgatgg
    98641 tgatcgcacc tttggattcc ggttcgtcca tttcaccgtc gccgaggaac gcgtaaacgg
    98701 tctgtttaga ggtatctttc aggccacggt gttccagata tttcaggaat ttagcctggt
    98761 aaatagcacc aatcggaccc agacccatag atacggtcgg gaactgccag aattccggca
    98821 tcaatttcgg gtgcggatag gaagagaggc cattgccgtg aacttcctga cggaagttat
    98881 ccagctgctc ttgagtcaga cgaccttcca ggaaagcacg agcgtacacg cccggggaga
    98941 tgtggccctg gaagtaaacc aggtcgccgc catcctgctc gttgcgtgca cggaagaagt
    99001 ggttaaagca cacatcataa atggttgcgg aagactggaa ggacgccatg tggccgccca
    99061 gttcgaggtc ttttttcgac gcacgcagaa ccgtcatgat ggcgttccag cggatagctg
    99121 aacgaatacg gcgttccagt tccagattac ccggatactc cggttgttct tcaacgggga
    99181 tggtgttgat gtagttgctg ataccggtgc ctgcggctac gttgacaccg cctttgcggg
    99241 cttcagcaag cagttggtcg atcagatact gagcacgctc aacaccttct tcacggatga
    99301 ccgattcgat cgcctggagc cagtcgcgag tttcgatcgg atccacgtca tttgggaaac
    99361 gttctgacat gggttattcc ttatctatct aataacgttg agttttctgg aacctgtccc
    99421 attgaactct cgccggaaag ctcaataaga caggttctac gtttagttgc cgcgctttta
    99481 tatgcgcttg atttacaaca tcttctggat aatttttacc agaaaaatca ctaattcttt
    99541 cgttgctcca gacgacgcag agaacgctca cggcggcttt cttcacggct tctgtcgagc
    99601 aaaatttctt cgataaaggc cagatggcga tgcgatgctt cgcgcgcttc ttccggctta
    99661 ccggccataa tcgcttcaaa tatgcgggtg cggtgactac tcaccagcgg cagcatctcg
    99721 cgacgcgaat agagcaattc gaagttctgg cggacattct gggccaacat cggctccata
    99781 caccttagca gatgaagcag aaccacattg tgggccgctt cggtgacggc aatctgatac
    99841 tggagtacgg cgtttgattc cgcgtccaga tcgccagact gctgcgccag ttctatggcg
    99901 tggtggagtt cacggatgcg ttccttgtct tcatcggtac tacgcagcgc ggcgtaataa
    99961 gcggcgatac cttccagggc gtgtcgtgtt tcgagcaagt catactgtga ttcaggatgg
   100021 tcggagagca gctccaccag cggatcgctg aagctttgcc ataggctgct ctggacaaaa
   100081 gtgccgccac cctggcgacg aagcaacaag cccttcgctt cgagacgttg aatcgcctca
   100141 cgcaatgagg gacgggagac gtcaaactgt tttgccagtt cgcgttccgg tgggagtttt
   100201 tcgcccgggc ggagagtgcc ttcgaggatc aaaaactcca gttgctgctc aatcacatcg
   100261 gagagttttg gttggcggat tttgctgtag gccatgagtt cctgtcttaa gccacttgcc
   100321 gaagtcaatt ggtcttacca atttcatgtc tgtgacgcta aagtaacaaa gtattcacct
   100381 tatgtccata caggttttga ttgaaatcat gaaactgtgc acattttaac aacttgacat
   100441 atataacgtt tcaaagttgt aactatgcac aaatgtagac tttacgtagg aaagggattt
   100501 tttaacgatt attaaactat aaaaatccga attgaaccga ttcact
//
LOCUS       DAZFAB010000004        97943 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZFAB010000004 DAZFAB010000000
VERSION     DAZFAB010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 97943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 97943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..97943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.004"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            10..678
                     /gene="ydhX"
                     /locus_tag="ACHJWA_000317"
     CDS             10..678
                     /gene="ydhX"
                     /locus_tag="ACHJWA_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310405.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO4306994.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHAPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            675..1460
                     /gene="phsC"
                     /locus_tag="ACHJWA_000318"
     CDS             675..1460
                     /gene="phsC"
                     /locus_tag="ACHJWA_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFO4306995.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGERIYLYSKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINGVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            1464..2276
                     /gene="ydhT"
                     /locus_tag="ACHJWA_000319"
     CDS             1464..2276
                     /gene="ydhT"
                     /locus_tag="ACHJWA_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFO4306996.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGERAFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGKRCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRAGVRYRLNEQHQFVEC"
     gene            complement(2288..3892)
                     /gene="ydhS"
                     /locus_tag="ACHJWA_000320"
     CDS             complement(2288..3892)
                     /gene="ydhS"
                     /locus_tag="ACHJWA_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFO4306997.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPIYCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARKQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EYGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDQFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(4018..4323)
                     /gene="ydhR"
                     /locus_tag="ACHJWA_000321"
     CDS             complement(4018..4323)
                     /gene="ydhR"
                     /locus_tag="ACHJWA_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFO4306998.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTAQLKKLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(4431..4507)
                     /locus_tag="ACHJWA_000322"
     tRNA            complement(4431..4507)
                     /locus_tag="ACHJWA_000322"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4471..4473),aa:Val,seq:gac)
     gene            complement(4512..4588)
                     /locus_tag="ACHJWA_000323"
     tRNA            complement(4512..4588)
                     /locus_tag="ACHJWA_000323"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4552..4554),aa:Val,seq:gac)
     gene            4896..6152
                     /gene="ydhQ"
                     /locus_tag="ACHJWA_000324"
     CDS             4896..6152
                     /gene="ydhQ"
                     /locus_tag="ACHJWA_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFO4306999.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKANDTLLADGTMNNLGGEDSDTIVENGAIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDSVTFSIGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVQITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(6193..7566)
                     /gene="mdtK"
                     /locus_tag="ACHJWA_000325"
     CDS             complement(6193..7566)
                     /gene="mdtK"
                     /locus_tag="ACHJWA_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFO4307000.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRYLQRLPSAI
                     ILQRASR"
     gene            7781..8422
                     /gene="ribE"
                     /locus_tag="ACHJWA_000326"
     CDS             7781..8422
                     /gene="ribE"
                     /locus_tag="ACHJWA_000326"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFO4307001.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(8462..9610)
                     /gene="cfa"
                     /locus_tag="ACHJWA_000327"
     CDS             complement(8462..9610)
                     /gene="cfa"
                     /locus_tag="ACHJWA_000327"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFO4307002.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFTVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFVAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(9901..11112)
                     /gene="punC"
                     /locus_tag="ACHJWA_000328"
     CDS             complement(9901..11112)
                     /gene="punC"
                     /locus_tag="ACHJWA_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFO4307003.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAAMLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            11225..12157
                     /gene="punR"
                     /locus_tag="ACHJWA_000329"
     CDS             11225..12157
                     /gene="punR"
                     /locus_tag="ACHJWA_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFO4307004.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(12154..13179)
                     /gene="purR"
                     /locus_tag="ACHJWA_000330"
     CDS             complement(12154..13179)
                     /gene="purR"
                     /locus_tag="ACHJWA_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFO4307005.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            13478..13567
                     /gene="cydH"
                     /locus_tag="ACHJWA_000331"
                     /gene_synonym="ynhF"
     CDS             13478..13567
                     /gene="cydH"
                     /locus_tag="ACHJWA_000331"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFO4307006.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            13733..14902
                     /gene="ydhP"
                     /locus_tag="ACHJWA_000332"
     CDS             13733..14902
                     /gene="ydhP"
                     /locus_tag="ACHJWA_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4307007.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNKFGAAISMAVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(15048..15629)
                     /gene="sodB"
                     /locus_tag="ACHJWA_000333"
     CDS             complement(15048..15629)
                     /gene="sodB"
                     /locus_tag="ACHJWA_000333"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFO4307008.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(15757..16572)
                     /gene="mepH"
                     /locus_tag="ACHJWA_000334"
     CDS             complement(15757..16572)
                     /gene="mepH"
                     /locus_tag="ACHJWA_000334"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFO4307009.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHTVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            16907..17254
                     /gene="grxD"
                     /locus_tag="ACHJWA_000335"
     CDS             16907..17254
                     /gene="grxD"
                     /locus_tag="ACHJWA_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFO4307010.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(17305..21921)
                     /gene="lhr"
                     /locus_tag="ACHJWA_000336"
     CDS             complement(17305..21921)
                     /gene="lhr"
                     /locus_tag="ACHJWA_000336"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFO4307011.1"
                     /translation="MADNPNPSSPLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLKGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAADAAHFE
                     STSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVELVIQVATPLSVASGLQRIGRAGHQIGGISKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQHTVAAAAMDALQVDEWYSRVRRAAPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEQA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTIANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARALLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPAILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEIASYLE
                     NLLTVKRIFPTMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVHDYSPEVLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEADQANRSALQQAIVAVLADGGAWFAQQISQRIRDKIGESVDPSALQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPRVSYNTPN
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQAAQTRHYTPVALSANDPANVWGNL
                     LPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFHALTT
                     ALRREPRLRFTLTEVNDLPVWQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(22014..22661)
                     /gene="rnt"
                     /locus_tag="ACHJWA_000337"
     CDS             complement(22014..22661)
                     /gene="rnt"
                     /locus_tag="ACHJWA_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFO4307012.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(22764..23171)
                     /gene="gloA"
                     /locus_tag="ACHJWA_000338"
     CDS             complement(22764..23171)
                     /gene="gloA"
                     /locus_tag="ACHJWA_000338"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFO4307013.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(23252..24349)
                     /gene="nemA"
                     /locus_tag="ACHJWA_000339"
     CDS             complement(23252..24349)
                     /gene="nemA"
                     /locus_tag="ACHJWA_000339"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFO4307014.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPSI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(24386..24985)
                     /gene="nemR"
                     /locus_tag="ACHJWA_000340"
     CDS             complement(24386..24985)
                     /gene="nemR"
                     /locus_tag="ACHJWA_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFO4307015.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYATYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRDSHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRNSEPLENALAHVKTIIATPAV"
     gene            25088..25327
                     /gene="ydhL"
                     /locus_tag="ACHJWA_000341"
     CDS             25088..25327
                     /gene="ydhL"
                     /locus_tag="ACHJWA_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFO4307016.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            25376..26272
                     /gene="ydhF"
                     /locus_tag="ACHJWA_000342"
                     /pseudo
     CDS             25376..26272
                     /gene="ydhF"
                     /locus_tag="ACHJWA_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
     gene            26353..26874
                     /gene="sodC"
                     /locus_tag="ACHJWA_000343"
     CDS             26353..26874
                     /gene="sodC"
                     /locus_tag="ACHJWA_000343"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFO4307017.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSLGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(26875..28887)
                     /gene="ydhK"
                     /locus_tag="ACHJWA_000344"
     CDS             complement(26875..28887)
                     /gene="ydhK"
                     /locus_tag="ACHJWA_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFO4307018.1"
                     /translation="MNASSWSLRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETTDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTALASSQTDVYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSDVDDHARLPRTSGLARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYNFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGIVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(28887..29744)
                     /gene="ydhJ"
                     /locus_tag="ACHJWA_000345"
     CDS             complement(28887..29744)
                     /gene="ydhJ"
                     /locus_tag="ACHJWA_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFO4307019.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVDAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(29747..29983)
                     /gene="ydhI"
                     /locus_tag="ACHJWA_000346"
     CDS             complement(29747..29983)
                     /gene="ydhI"
                     /locus_tag="ACHJWA_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFO4307020.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            30184..30618
                     /gene="slyA"
                     /locus_tag="ACHJWA_000347"
     CDS             30184..30618
                     /gene="slyA"
                     /locus_tag="ACHJWA_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFO4307021.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(30665..31132)
                     /gene="slyB"
                     /locus_tag="ACHJWA_000348"
     CDS             complement(30665..31132)
                     /gene="slyB"
                     /locus_tag="ACHJWA_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFO4307022.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            31405..32514
                     /gene="anmK"
                     /locus_tag="ACHJWA_000349"
     CDS             31405..32514
                     /gene="anmK"
                     /locus_tag="ACHJWA_000349"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFO4307023.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVKALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            32612..32941
                     /gene="mliC"
                     /locus_tag="ACHJWA_000350"
     CDS             32612..32941
                     /gene="mliC"
                     /locus_tag="ACHJWA_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFO4307024.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGLSASGARYSDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            33000..33656
                     /gene="pdxH"
                     /locus_tag="ACHJWA_000351"
     CDS             33000..33656
                     /gene="pdxH"
                     /locus_tag="ACHJWA_000351"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFO4307025.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGSWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            33785..35059
                     /gene="tyrS"
                     /locus_tag="ACHJWA_000352"
     CDS             33785..35059
                     /gene="tyrS"
                     /locus_tag="ACHJWA_000352"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFO4307026.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            35121..35981
                     /gene="pdxY"
                     /locus_tag="ACHJWA_000353"
     CDS             35121..35981
                     /gene="pdxY"
                     /locus_tag="ACHJWA_000353"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HFO4307027.1"
                     /translation="MKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNHT
                     QYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKAA
                     NPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHPVNNV
                     EEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQP
                     VGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIAK
                     PEHYFSATKL"
     gene            complement(36025..36630)
                     /gene="gstA"
                     /locus_tag="ACHJWA_000354"
     CDS             complement(36025..36630)
                     /gene="gstA"
                     /locus_tag="ACHJWA_000354"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFO4307028.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(36736..38238)
                     /gene="dtpA"
                     /locus_tag="ACHJWA_000355"
     CDS             complement(36736..38238)
                     /gene="dtpA"
                     /locus_tag="ACHJWA_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFO4307029.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHTILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(38849..39484)
                     /gene="nth"
                     /locus_tag="ACHJWA_000356"
     CDS             complement(38849..39484)
                     /gene="nth"
                     /locus_tag="ACHJWA_000356"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFO4307030.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNS
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(39484..40179)
                     /gene="rsxE"
                     /locus_tag="ACHJWA_000357"
     CDS             complement(39484..40179)
                     /gene="rsxE"
                     /locus_tag="ACHJWA_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFO4307031.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(40183..40803)
                     /gene="rsxG"
                     /locus_tag="ACHJWA_000358"
     CDS             complement(40183..40803)
                     /gene="rsxG"
                     /locus_tag="ACHJWA_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFO4307032.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGNFDQFTGATITPRAVVNAVKRAGLYAQTLPEQLSQLPACGE"
     gene            complement(40807..41865)
                     /gene="rsxD"
                     /locus_tag="ACHJWA_000359"
     CDS             complement(40807..41865)
                     /gene="rsxD"
                     /locus_tag="ACHJWA_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFO4307033.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(41866..44184)
                     /gene="rsxC"
                     /locus_tag="ACHJWA_000360"
     CDS             complement(41866..44184)
                     /gene="rsxC"
                     /locus_tag="ACHJWA_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFO4307034.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRSRRREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSHDISMRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVTDPRKTAVEAAIARA
                     KARKLEQQQANAEPEQQVDPRKAAVEAAIARAKARKLEQQQANAEPEQQVDPRKAAVE
                     AAIARAKARKLEQQQANAEPEEPVDPRKAAVEAAIARAKARKLEQQQANAEPEEPVDP
                     RKAAVEAAITRAKARKLEQQQANAEPEEQVDPRKAAVAAAIARAKARKLEQQQANAEP
                     EEQVDPRKAAVAAAIARVQAKKAVQQKVVNED"
     gene            complement(44177..44755)
                     /gene="rsxB"
                     /locus_tag="ACHJWA_000361"
     CDS             complement(44177..44755)
                     /gene="rsxB"
                     /locus_tag="ACHJWA_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFO4307035.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(44755..45336)
                     /gene="rsxA"
                     /locus_tag="ACHJWA_000362"
     CDS             complement(44755..45336)
                     /gene="rsxA"
                     /locus_tag="ACHJWA_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFO4307036.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(45413..45853)
                     /gene="ydgK"
                     /locus_tag="ACHJWA_000363"
     CDS             complement(45413..45853)
                     /gene="ydgK"
                     /locus_tag="ACHJWA_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFO4307037.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGAQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(45939..46154)
                     /gene="ydgT"
                     /locus_tag="ACHJWA_000364"
     CDS             complement(45939..46154)
                     /gene="ydgT"
                     /locus_tag="ACHJWA_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFO4307038.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(46427..46552)
                     /gene="blr"
                     /locus_tag="ACHJWA_000365"
     CDS             complement(46427..46552)
                     /gene="blr"
                     /locus_tag="ACHJWA_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFO4307039.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSTTVQHK"
     gene            46795..47835
                     /gene="ydgJ"
                     /locus_tag="ACHJWA_000366"
     CDS             46795..47835
                     /gene="ydgJ"
                     /locus_tag="ACHJWA_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO4307040.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(47871..48872)
                     /gene="add"
                     /locus_tag="ACHJWA_000367"
     CDS             complement(47871..48872)
                     /gene="add"
                     /locus_tag="ACHJWA_000367"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFO4307041.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLNAEEKRALREKVAAK"
     gene            complement(48976..50148)
                     /gene="malY"
                     /locus_tag="ACHJWA_000368"
     CDS             complement(48976..50148)
                     /gene="malY"
                     /locus_tag="ACHJWA_000368"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFO4307042.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDPQSVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(50158..51750)
                     /gene="malX"
                     /locus_tag="ACHJWA_000369"
     CDS             complement(50158..51750)
                     /gene="malX"
                     /locus_tag="ACHJWA_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFO4307043.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLLPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVVAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            51925..52953
                     /gene="malI"
                     /locus_tag="ACHJWA_000370"
     CDS             51925..52953
                     /gene="malI"
                     /locus_tag="ACHJWA_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFO4307044.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            53065..53832
                     /gene="hdhA"
                     /locus_tag="ACHJWA_000371"
     CDS             53065..53832
                     /gene="hdhA"
                     /locus_tag="ACHJWA_000371"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFO4307045.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            54061..54651
                     /gene="uidR"
                     /locus_tag="ACHJWA_000372"
     CDS             54061..54651
                     /gene="uidR"
                     /locus_tag="ACHJWA_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFO4307046.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            55042..56855
                     /gene="uidA"
                     /locus_tag="ACHJWA_000373"
                     /pseudo
     CDS             55042..56855
                     /gene="uidA"
                     /locus_tag="ACHJWA_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
     gene            56852..58225
                     /gene="uidB"
                     /locus_tag="ACHJWA_000374"
     CDS             56852..58225
                     /gene="uidB"
                     /locus_tag="ACHJWA_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFO4307047.1"
                     /translation="MNQQLSWRTIVGYSLGDIANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHGSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            58264..59528
                     /gene="uidC"
                     /locus_tag="ACHJWA_000375"
                     /pseudo
     CDS             58264..59528
                     /gene="uidC"
                     /locus_tag="ACHJWA_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
     gene            complement(59573..61081)
                     /gene="ydgA"
                     /locus_tag="ACHJWA_000376"
     CDS             complement(59573..61081)
                     /gene="ydgA"
                     /locus_tag="ACHJWA_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFO4307048.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPVVPAIPQQ"
     gene            complement(61182..62357)
                     /gene="manA"
                     /locus_tag="ACHJWA_000377"
     CDS             complement(61182..62357)
                     /gene="manA"
                     /locus_tag="ACHJWA_000377"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFO4307049.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HKSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPDDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            62556..64202
                     /gene="fumB"
                     /locus_tag="ACHJWA_000378"
     CDS             62556..64202
                     /gene="fumB"
                     /locus_tag="ACHJWA_000378"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO4307050.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSLSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQALGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            64345..65748
                     /gene="fumC"
                     /locus_tag="ACHJWA_000379"
     CDS             64345..65748
                     /gene="fumC"
                     /locus_tag="ACHJWA_000379"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFO4307051.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(65745..66674)
                     /gene="tus"
                     /locus_tag="ACHJWA_000380"
     CDS             complement(65745..66674)
                     /gene="tus"
                     /locus_tag="ACHJWA_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFO4307052.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSQALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(66750..68051)
                     /gene="rstB"
                     /locus_tag="ACHJWA_000381"
     CDS             complement(66750..68051)
                     /gene="rstB"
                     /locus_tag="ACHJWA_000381"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFO4307053.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(68055..68774)
                     /gene="rstA"
                     /locus_tag="ACHJWA_000382"
     CDS             complement(68055..68774)
                     /gene="rstA"
                     /locus_tag="ACHJWA_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFO4307054.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDIQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            68903..69238
                     /gene="ydgC"
                     /locus_tag="ACHJWA_000383"
     CDS             68903..69238
                     /gene="ydgC"
                     /locus_tag="ACHJWA_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFO4307055.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(69235..69957)
                     /gene="folM"
                     /locus_tag="ACHJWA_000384"
     CDS             complement(69235..69957)
                     /gene="folM"
                     /locus_tag="ACHJWA_000384"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFO4307056.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(69994..71376)
                     /gene="ydgI"
                     /locus_tag="ACHJWA_000385"
     CDS             complement(69994..71376)
                     /gene="ydgI"
                     /locus_tag="ACHJWA_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO4307057.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDIFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(71562..72506)
                     /gene="ydgH"
                     /locus_tag="ACHJWA_000386"
     CDS             complement(71562..72506)
                     /gene="ydgH"
                     /locus_tag="ACHJWA_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFO4307058.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            73030..74562
                     /gene="pntA"
                     /locus_tag="ACHJWA_000387"
     CDS             73030..74562
                     /gene="pntA"
                     /locus_tag="ACHJWA_000387"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFO4307059.1"
                     /translation="MRIGIPRERLTNEPRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGCFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKNGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQVAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            74573..75961
                     /gene="pntB"
                     /locus_tag="ACHJWA_000388"
     CDS             74573..75961
                     /gene="pntB"
                     /locus_tag="ACHJWA_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFO4307060.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(75986..77020)
                     /gene="tqsA"
                     /locus_tag="ACHJWA_000389"
     CDS             complement(75986..77020)
                     /gene="tqsA"
                     /locus_tag="ACHJWA_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFO4307061.1"
                     /translation="MAKPLITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSTLNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFKQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            77432..77797
                     /gene="mdtJ"
                     /locus_tag="ACHJWA_000390"
     CDS             77432..77797
                     /gene="mdtJ"
                     /locus_tag="ACHJWA_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFO4307062.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            77784..78113
                     /gene="mdtI"
                     /locus_tag="ACHJWA_000391"
     CDS             77784..78113
                     /gene="mdtI"
                     /locus_tag="ACHJWA_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFO4307063.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(78152..78973)
                     /gene="ydgD"
                     /locus_tag="ACHJWA_000392"
     CDS             complement(78152..78973)
                     /gene="ydgD"
                     /locus_tag="ACHJWA_000392"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFO4307064.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYDIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKAAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(79073..79156)
                     /locus_tag="ACHJWA_000393"
     CDS             complement(79073..79156)
                     /locus_tag="ACHJWA_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4307065.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(79249..79557)
                     /gene="asr"
                     /locus_tag="ACHJWA_000394"
     CDS             complement(79249..79557)
                     /gene="asr"
                     /locus_tag="ACHJWA_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFO4307066.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAKKHSHQQPAKPAAQPAA
                     "
     gene            79828..79932
                     /locus_tag="ACHJWA_000395"
     CDS             79828..79932
                     /locus_tag="ACHJWA_000395"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFO4307067.1"
                     /translation="MNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            complement(79981..81234)
                     /gene="ynfM"
                     /locus_tag="ACHJWA_000396"
     CDS             complement(79981..81234)
                     /gene="ynfM"
                     /locus_tag="ACHJWA_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4307068.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKSLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            81341..82234
                     /gene="ynfL"
                     /locus_tag="ACHJWA_000397"
     CDS             81341..82234
                     /gene="ynfL"
                     /locus_tag="ACHJWA_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4307069.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            82369..83589
                     /gene="mlc"
                     /locus_tag="ACHJWA_000398"
                     /gene_synonym="dgsA"
     CDS             82369..83589
                     /gene="mlc"
                     /locus_tag="ACHJWA_000398"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFO4307070.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            83714..84409
                     /gene="bioD"
                     /locus_tag="ACHJWA_000399"
     CDS             83714..84409
                     /gene="bioD"
                     /locus_tag="ACHJWA_000399"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO4307071.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TEKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(84362..85654)
                     /gene="clcB"
                     /locus_tag="ACHJWA_000400"
     CDS             complement(84362..85654)
                     /gene="clcB"
                     /locus_tag="ACHJWA_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFO4307072.1"
                     /translation="MHHLHTYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(85812..86426)
                     /gene="dmsD"
                     /locus_tag="ACHJWA_000401"
     CDS             complement(85812..86426)
                     /gene="dmsD"
                     /locus_tag="ACHJWA_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFO4307073.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(86469..87323)
                     /gene="ynfH"
                     /locus_tag="ACHJWA_000402"
     CDS             complement(86469..87323)
                     /gene="ynfH"
                     /locus_tag="ACHJWA_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFO4307074.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKTDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFVTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLSSIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(87325..87942)
                     /gene="ynfG"
                     /locus_tag="ACHJWA_000403"
     CDS             complement(87325..87942)
                     /gene="ynfG"
                     /locus_tag="ACHJWA_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFO4307075.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(87953..90376)
                     /gene="ynfF"
                     /locus_tag="ACHJWA_000404"
     CDS             complement(87953..90376)
                     /gene="ynfF"
                     /locus_tag="ACHJWA_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HFO4307076.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLISENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTNEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            complement(90437..92863)
                     /gene="ynfE"
                     /locus_tag="ACHJWA_000405"
     CDS             complement(90437..92863)
                     /gene="ynfE"
                     /locus_tag="ACHJWA_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfE"
                     /protein_id="HFO4307077.1"
                     /translation="MSKNERMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQASEQVVWGACSVNCGSRCALRLHVKDNEVAWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNITRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKSREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDKTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPER
                     STFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAQKRGIANGDMVRVYNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            complement(93062..93367)
                     /gene="ynfD"
                     /locus_tag="ACHJWA_000406"
     CDS             complement(93062..93367)
                     /gene="ynfD"
                     /locus_tag="ACHJWA_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFO4307078.1"
                     /translation="MKLSTCYAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            93475..94185
                     /gene="ynfC"
                     /locus_tag="ACHJWA_000407"
     CDS             93475..94185
                     /gene="ynfC"
                     /locus_tag="ACHJWA_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFO4307079.1"
                     /translation="MKYKLLPCLLAILLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVGCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            complement(94188..94748)
                     /gene="speG"
                     /locus_tag="ACHJWA_000408"
     CDS             complement(94188..94748)
                     /gene="speG"
                     /locus_tag="ACHJWA_000408"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HFO4307080.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFSVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(94783..95124)
                     /gene="ynfB"
                     /locus_tag="ACHJWA_000409"
     CDS             complement(94783..95124)
                     /gene="ynfB"
                     /locus_tag="ACHJWA_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFO4307081.1"
                     /translation="MKITLSKRIDLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTGNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            95259..95585
                     /gene="ynfA"
                     /locus_tag="ACHJWA_000410"
     CDS             95259..95585
                     /gene="ynfA"
                     /locus_tag="ACHJWA_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFO4307082.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGPLIALCGMLIIV
                     VGWGRT"
     gene            95791..95877
                     /locus_tag="ACHJWA_000411"
                     /pseudo
     CDS             95791..95877
                     /locus_tag="ACHJWA_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(96352..96539)
                     /locus_tag="ACHJWA_000412"
                     /pseudo
     CDS             complement(96352..96539)
                     /locus_tag="ACHJWA_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686441.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
     gene            complement(96574..96825)
                     /locus_tag="ACHJWA_000413"
     CDS             complement(96574..96825)
                     /locus_tag="ACHJWA_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO4307083.1"
                     /translation="MSEVIMIVSPGKWVSEEQLIALKGIKKGTLKKAREKSFMEGREY
                     KHVAHDGMPWDNSPCFYNLEEIDRWIERQASARPRRHLT"
     gene            complement(96898..>97943)
                     /locus_tag="ACHJWA_000414"
     CDS             complement(96898..>97943)
                     /locus_tag="ACHJWA_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO4307084.1"
                     /translation="ELLAVSRGEFVEGISDPNDPKWVKGIQTRDSVNQNQQETEQNDQ
                     KAEQNSPNTQQNEPETKQPEPVVQQEPEKICTACGQSGGGNCPDCGAVMGDATYQEIF
                     DGENQPEVQENDPEEMEGTAHQHKENTGGNQHHASDSETGEASDPLIKANGHHNLTST
                     SRAGIHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTIDLDTAGGVIDRDT
                     MKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVHVWGNGANFDNTILRR
                     SYERQGSPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNALDDARYQAKYVS
                     AIWQKLIPSQADS"
BASE COUNT        24455 a        24221 c        25285 g        23982 t
ORIGIN      
        1 gataatcaga tgtccttcac tcgacgcaaa tttgttctgg ggatgggaac agtaatcttt
       61 tttactggtt ctgcgtcttc tctgttagcg aacacgaggc aagaaaagga agttcggtat
      121 gccatgattc atgacgagtc acggtgcaat ggctgtaata tttgcgcccg cgcatgccgt
      181 aaaactaacc acgcccccgc tcagggaagc cgtttatcga tagcacacat tccagtcacc
      241 gataacgaca acgaaacgca gtatcacttt tttcgccagt catgtcagca ctgcgaagat
      301 gctccgtgca tcgacgtatg cccgacaggg gcgtcgtggc gtgatgagca ggggatcgtg
      361 cgggtagaaa aatcgcagtg catcggctgc agttattgca ttggcgcttg tccgtaccag
      421 gttcgctacc ttaaccctgt cactaaagtg gcagataaat gtgatttctg cgctgagtcc
      481 cgactggcaa aaggctttcc gcccatttgc gtcagcgctt gcccggaaca cgcattgatc
      541 tttggtcgcg aagatagccc tgaaattcag gcgtggttgc aggataataa atactatcaa
      601 tatcagctac ctggtgccgg gaaaccgcat ctgtatcgcc ggttcggtca acatttgatt
      661 aaaaaggaaa atgtatgaac ccgtcgcaac atgctgaaca gtttcagagc cagttagcga
      721 actatgtgcc acagttcact cccgaatttt ggccggtgtg gttgatcatt gccggagtat
      781 tgctggttgg gatgtggttg gtgctggggc tgcacgcctt gcttcgtgct cgtggcgtga
      841 agaaatcagc caccgatcat ggtgagagga tttatcttta cagcaaagcg gtcagattat
      901 ggcactggtc gaatgcgtta ctctttgtat tgttgctggc aagtgggctg ataaatcact
      961 ttgcgctggt gggcgcaact gcggttaaaa gtctggttgc ggtgcatgaa gtttgcggat
     1021 ttttgttact ggcatgctgg ctcggctttg ttctgatcaa tggcgttggg ggtaatggcc
     1081 accactatcg cattcgtcgt cagggttggc tggagcgggc ggcaaaacaa acgcgatttt
     1141 atttgtttgg cattatgcag ggggaagaac atcctttccc ggcaacaacc cagtctaaat
     1201 ttaatccctt acagcaggtt gcctatgttg gtgtcatgta tggattgctg ccgttgttac
     1261 tattgacggg gctgctgtgt ctctatccgc aagccgtggg agatgtgttt cctggcgtaa
     1321 gatactggtt attgcaggcg cattttgctc tggcatttat aagcctcttt tttatcttcg
     1381 gtcatcttta tctttgcacc acggggcgta cgccacacga aacctttaaa agcatggtcg
     1441 atggctatca ccggcactaa cgaatgatca ttacacgagc agacctgcga gaatggcgta
     1501 tcggagcggt gatgtatcgc tggttcctgc gccatttccc acgcggcggt agttatgccg
     1561 atattcatca tgcactgatt gaggaaggat atacggactg ggcggagagt ctggttgagt
     1621 atgcgtggaa aaaatggctg gcggatgaaa acttcgcgca tcaggaagtt tcctctatgc
     1681 aaaaactggc cacagacccc ggagagagag cattttgcag tcagtttgcc cgcagcgacg
     1741 atcatgctcg catcggctgt tgtgaggata acgccagaat cgcaaccgct gggtatgctg
     1801 cacaaattgc cagtatgggt tatagcgtgc ggattggaag tgtcggcttt aacagtcaca
     1861 ttggcagctc cggtgagcgc gcacgggttg ctgtaaccgg caattcttcg cggataagca
     1921 gcgctggaga tagcagtcgg attgcgaata ccggaatgcg agtacgcgtt tgtacattgg
     1981 gtaaacggtg tcatgtcgcc agtaatggtg acctggtgca aatcgccagc tttggcgcaa
     2041 acgccagaat tgccaacagc ggcgacaatg tacacatcat tgccagcggt gaaaattcaa
     2101 cggtagtgag cacgggcgtg gtcgactcaa ttattctcgg gccagggggc agcgccgcac
     2161 tggcatatca tgacggcgaa agagttcgct tcgccgtcgc cattgaaggt gaaaacaata
     2221 ttcgagccgg agtccgctat cgtcttaatg aacagcatca gtttgttgag tgctgatagc
     2281 tttaccttta atcaaacgaa agacgccgac gagcacggga tgcaggcttt acgaccgccc
     2341 gagccatcgc ctcaccaatt tctgcacatg ccgtaagtcc ctgaacgaaa ggctggtcgt
     2401 gcatcaacca gggcaacgcg ccgaacgcta cgcgcccacg aatatcttcg ggttgctgta
     2461 acgtataatc ttcaccaaca tcagggattt catcccctgt tttctgtaat tgttcgcgta
     2521 gtccagggaa aggaatatct ttcactttaa gcggacgctg cccgcgggca tcaataaaaa
     2581 cgtcaaacga gtagctgttg tcttccgttt tcaggacggt gcgcgactcg ttaatttcca
     2641 ttttgtagtc ttcaccgaga gcgagaatat gaatgattcc cgcttcacgt aaggcaagta
     2701 ggcgacgaat agactctgaa gggattgccg cataattatc gatgaaaacc cgggcaaggc
     2761 ctttactgaa ccgtttatgg tcgtgttcat tcagatgtgg aacaatttcc tgtacggctt
     2821 catgcaggcg cagaatgaca taacgccagg gaacagtatg tttttctcgt ttattgcgtt
     2881 cgacttcctg gagatttttt tctgcccagt caaattggtc gcgttgtttg cgctcggcaa
     2941 accaggcttg agcaaaggaa tcgacattca ggctctctaa ggctatgcgt tgactccagt
     3001 ctggatcagc aaacttgatt tcctctacta tcaatctaaa tacccgatcc aaaaggccat
     3061 attcgccttt ttgaatctca gcatttaatg cctgatcagt gacgatgtgt aagggctcgt
     3121 agggaatagg gcaatagaaa tcggcttcgg gtaaaatacc cgtgcgcgac attaacgtaa
     3181 tatttagctt ttcacttgcg ttatcgcggt gaaaaacgac gtgttgttta tcatcttcaa
     3241 tgaacgaacc atgctgaata gccactgcca ttgccgcatc cagtccgctc aaggatgttc
     3301 ccataatacc cacgttacac gcatcgacct ttgcttccat caagcctgac cacgggcttg
     3361 gaaaatacgt tcgggttgct tcttcttcat caggccagac gtgacccgtg gcgatcaccg
     3421 ctaaatcaaa cgtctcgctg ggtaaatcct gatttgtagc gagcatgacg ccagcatttg
     3481 taatttgcag atcggtaacc tggcatgatt cataaacagc cactgcaaat ttttgctttc
     3541 gtgcctggtc tactaatcgt aaaaattgat cgcggaaata ttcgcccagc agaattcgcg
     3601 gtaaaaactg acgatcgtgc aaggtttctt ttttaacgcc ataacgctgg agatgactgg
     3661 cttcttgctt ttgtagccat tcgagatacg tacaataaat cggcggtatt tcaatactgg
     3721 caatatttgc cagcatcatt tttgagtttt cctcatcact gtatggcatc ccgacaccgg
     3781 cctcgtcagc ctgctcgaag atagaaatag aaagtggagt ttgttgctgt agaagcgaga
     3841 ataaggtgta gatccccgta ggcccggcac ccacaattgc aatttttttc attgttactt
     3901 acctgtacgc attctttcga atttagtgtt atcagataag tttggttgag atttttagaa
     3961 tttgagaaat ttcagacagg gatgaaaagg gcgctaaccg cccttgatta aattctgtca
     4021 ggcgagtttt gcctgattga tttgactcag tggctcattg acatcgaaaa ctttggcgac
     4081 aacttcctca acgccgagtt ttttcagttg ggcagtgtgt ttttccagat aggcaagggc
     4141 gcttttttca tcagtgaaaa ggtagatccc accggcttcg tggttctttt cactttctgt
     4201 ccataccttc cacagaaaac caggttcctg attaatcgac tctgcaagtg gtttaagctg
     4261 ctcagccatt gcgtcgccaa atgggccatt aaaagcaaaa tgaagttgta acaaagttgc
     4321 catagcgatt ctccttctga taacgcatac gaagacagtg taaatctttt aacgtattgg
     4381 atacagagaa gaagcagagg gaaaaaggag aagaccagat taagaaaatc tggtgcgtcc
     4441 gagtggactc gaaccaccga cccccaccat gtcaaggtgg tgctctaacc aactgagcta
     4501 cggacgcagg atggtgcgtt caattggact cgaaccaacg acccccacca tgtcaaggtg
     4561 gtgctctaac caactgagct atgaacgcaa cgttgtaggt gacaacgggg acgaatatta
     4621 gcggcagagt gggaaggtgg caagaggcaa aacgtaattt tctgcgctat ttcgaccgtt
     4681 tgcagagctt ttaagcaaat tggctatatt ttgttgattt gcaagggcga tttttattca
     4741 ggatcgcatt tacatctgat acaacccaat aattcgtgta tcagagttta aacagagttg
     4801 tttgccacca tctcaaatct gtcgtggata aatcctccgt agcctttaca cttaggtagc
     4861 aataaaggca cacagatgca gcaaaggaat aaatcatggg atctgatgcg aaaaacttga
     4921 tgagcgacgg gaatgtgcaa attgttaaga ccggcgaggt cattggcgcg acgcaactta
     4981 ctgaaggcga gttgattgtt gaagctggcg gaagagccga aaataccgtg gtcacggggg
     5041 ctggctggtt gaaagtggca accggtggga tcgccaaatg cacacagtac ggtaacaatg
     5101 gcacgctatc ggtcagcgac ggtgccattg ccacagatat tgttcagtcc gagggaggcg
     5161 caattagtct ctctacgctc gctacggtta atggccgcca tcccgaaggt gaattcagcg
     5221 ttgatcaggg ttatgcctgc ggtttgttgc tggaaaatgg cggtaacctg cgtgtactgg
     5281 aaggacatcg cgcggaaaaa atcattctcg atcaagaggg cggcctgttg gttaatggga
     5341 caacctcagc ggtcgtggta gatgaaggtg gtgaattgtt ggtgtatcca ggtggggaag
     5401 ccagcaattg tgagattaat cagggcggcg tttttatgct ggcggggaaa gccaatgata
     5461 cgttgcttgc tgatggcacc atgaataatc tcggtggtga agactctgac actattgttg
     5521 agaatggagc catctatcgt ctggggacgg atggtcttca gctctacagt tccggtaaga
     5581 cgcaaaacct gtccgttaat gtgggtggtc gggctgaagt gcatgccggt acgctggaaa
     5641 atgcggtaat acaaggtgga acagtgatcc tgttgtcacc caccagcgcg gacgaaaatt
     5701 ttgtcgtaga ggaagatcgc gcaccggttg aactgaccgg gagtgttgca ttactggacg
     5761 gcgcttcaat gattattggc tatggcgcag atctgcaaca atcaacgatt actgtacagc
     5821 agggcggtgt attgattctc gacggcagta cggtaaaagg tgacagtgtc actttcagta
     5881 ttggtaacat caatctgaat ggcggaaaac tgtggctgat cactggtgcg gcaacgcatg
     5941 tgcagctgaa agtgaaacgc ctgcgcggag agggagcgat ttgcctgcaa accagtgcga
     6001 aagaaatttc acctgacttc atcaatgtga aaggggaagt taacggggat atacgcgttc
     6061 agataacaga tgccagtcgg caaactctgt gtaacgcact gaaactacag ccagacgaag
     6121 acgggattgg cgcaacgctc cagcctgcgt aaaaacacag gcgcaccccg tgaaggttgc
     6181 gccttgtctt tattagcggg atgctcgttg cagaatgatg gctgacggca gacgttgcag
     6241 gtaccgcata cgcagcatca tcataatggc tgccgacgtc aggccaataa taaagcctat
     6301 ccagaagcct gctggcccca taggttcaac gaccagatcg gtcagtgcca gaatatagcc
     6361 gcttggcaag cccagcaccc agtaagccgt aaaggtgata tagaaaatgg aacgcgtatc
     6421 tttataacca cgcaaaatcc cactgccaat cacctggatt gagtcagaaa tctgatatac
     6481 cgccgccagc aacatcaaat gcgccgccag cgttacaacc tcgggattgt cgttgtacaa
     6541 cagggcgatt tgctcccgca gtgaaaccgt gaatatggcc gtcagggttg ccatacagac
     6601 acccaccata agcccggtcc gcgcagcggt ttgcgcatcc agcgttgagc cctgacccag
     6661 acgataacct acgcggatag ttaccgctgc cgccagcgac attggaagca cgaacattag
     6721 tgaactaaag ttcagggcaa tctggtgtcc tgcgacatca acaataccga gcggagacac
     6781 taacagagcc acgacggcaa acagtgtcac ttcaaagaat agtgccagcg caatcggcaa
     6841 accgagttga atcagtcgtt tcataaccgc aggatcgggt ttcgcggtgc ctttttcgtt
     6901 acgaatatcg cgcatcgagc gggcgcgttt aatgtaagaa accatggcaa ggaacatgac
     6961 ccaatacacc gccgcagtag ccacgccaca accaacgcca ccgagctcag gcataccgaa
     7021 atgaccataa ataaagatat agttcaccgg aatgttcacc agcaggccga taaagcccat
     7081 taccataccc ggcttggttt ttgccagacc ttcacattgg ttacgggcaa cctggaagaa
     7141 aagatagccc ggcgcgcccc acagcaacgc acgcagataa cccacggctt tgtccgccag
     7201 agccggatcg atgttttcca tggagcggat aatgtaacct gcattccaca gcaccagcat
     7261 aatgagaacg gaaacaaaac ccgccagcca gaagccctgt cgcacctgat gcgcaatgcg
     7321 ctcacgtcga ccggaaccat ttaattgcgc gataaccggc gttaatgcca gcagcagtcc
     7381 gtgaccaaag aggatcgccg gaagccagat agaagtaccg atagcgaccg ccgccatgtc
     7441 ggtggcacta tagccgcccg ccatcacggt atcgacaaaa cccatcgcag tttgggcgat
     7501 ttgcgcgaga atcaccggga ttgctaatgc taataacaga cgcgcttcac tgatatactt
     7561 ctgcacgtga acacctttta tttgtagtta tatgagagac taaaaagccg cctgaatagg
     7621 cagcaaaaag aagaagcagg ggaaattcag tctattgtag cgaggattta ttaattctcc
     7681 agtgaaaaaa tagccattct taccccttca ctggtaaggg cgttttccac ctgctattgt
     7741 gctgaacaga atgtcttaac tgatttcagg agttgtaagt atgtttacgg ggattgtaca
     7801 gggcaccgca aaactggtgt cgattgacga gaaaccaaat tttcgcacac atgtggtgga
     7861 gttacccgac cacatgctgg acggtctgga aaccggtgcc tccgtggcgc ataacggttg
     7921 ctgcctgacc gtgacggaaa ttaacggcaa ccatgtcagt tttgacctga tgaaagaaac
     7981 gttgcgcatt accaatcttg gcgatttaaa agtgggggat tgggtaaacg ttgagcgtgc
     8041 ggcgaaattc agtgatgaaa ttggcggaca cttaatgtca ggtcatatta tgaccaccgc
     8101 tgaagtggcg aaaatattaa cctcggaaaa taatcgccag atctggttta aagtccagga
     8161 tagtcagttg atgaaatata tcctgtacaa aggatttatt ggcatcgacg gtattagcct
     8221 gaccgtcggc gaagtcacgc caacgcgttt ttgcgtccat ttaattccgg aaactctgga
     8281 acgcacgact cttgggaaga aaaaacttgg cgcacgcgtc aacattgaaa tcgatccaca
     8341 aactcaggca gtggtagata cggtagaacg tgtgctggcg gcacgagaaa atgccatgaa
     8401 tcaaccaggt acagaagcct gatctgaaat cgcccccgga gagggggcga tagaatagcc
     8461 tttagcgagc gactcgaagg ccgttttcaa caccgcgtga gaacaccacc tgccagagct
     8521 gaatatcacg ggcgcggaaa gcacctgcgc aggcattcag ataataggta aacatccgtt
     8581 taaagcgttc actgtagtta tccgcaattt ctggccatgc ggcgacgaat cgttcatacc
     8641 acgccatcaa cgtagtatcg taatcagcac cgaagttatg ccagtcttcc atcacaaagt
     8701 ggggttcgct ggactgagca atctggcgta cagagggcag gcaaccgttc gggaaaatat
     8761 atttattaat ccagggatca acattcagat cggttttttt cgaaccgata gtatggagca
     8821 ggaatatgcc ttccggtttc aaattacgat ccaccaccgt aaaataggta tcgtaatttt
     8881 tcggtccgac gtgctcgaac atccccacag aaacaatacg atcaaactgg tcgttcaggt
     8941 cacgataatc ttgcagcaaa atggtgacat ccaggccttc acagcgttcc tgagccattt
     9001 tttgctgttc ggcagaaatg gtgacgccca ccacgcttac gtcataatta gatgccatgt
     9061 agtgtgccag tccgccccag ccgcagccaa tatccagtac gcgcatccca ggttttaact
     9121 gcaatttttc acaaatcatt ttgagcttcg cctgctgggc agattccaga ttatcggcat
     9181 ctttccagta agcgcaggaa tattgcatga agggatcaag catgcggctg aacaagtcat
     9241 tacccaaatc gtaatgctct ttgccgacta tccaggcacg ttttttactc tgcagattga
     9301 agagacgggc gccggcaata cgcagcgtgt ctttgaaatg atgggggagt tggttctcga
     9361 gacctgcgcg taagactttg ctaaaaaaca tatccagtcg gtcacattcc caccagccat
     9421 ccatataact ttcgcctaac cccaaagagc cttcttgcag aacgcgttta aaaaaatcgg
     9481 ggtttttcac acgaatatcc gccggggcag aaccgttaat ggctataccg gcacggctaa
     9541 gtaattcgtt ggcgatacgg taccagttgt catccggtac actgacttct tctatacacg
     9601 atgaactcat agtttctcca tcactggtcg gtgatcagaa ccttaaaaca gcgtagacgc
     9661 tttttgggct ttgtgagaaa tctcgcagaa aaaaccgcga gtctataatc cgacgtagaa
     9721 cagagggaaa ggaggaaccc ttgccgaaat gaccatccat agtgagtcgg gaacgatcct
     9781 gtccccgtaa aaacataata tttatcgtag ctttaacaac tcgaaattag tataggccgc
     9841 taatttcaat catacaacaa aattttgtta gcagcctaag tataagtata tcgatataga
     9901 tcagtgtgat tcgctatgag cgacttcggc attgccatga ttctggcagc caacttcttc
     9961 acaacgttgc atcatgtaac ccagcgcgac cagcactact gttgataaca tcacgctggt
    10021 ggtggtgagc aatggcgtgc tgatactgat cagccaggaa actaccagac ttgcgaggaa
    10081 gcacagaccc agttgcagag tgttctgcaa cgctgcggcg cgaccagttg cgtgtgggaa
    10141 gggacgcaga gcctgggcga caacaatagg gtagatcgcg ccattagcaa tcgccatcac
    10201 acagaatggg atcaggattt cgaccagcga cacatggcta atgaagcccg cagcccaggt
    10261 cgcaatgacg ctgacagcaa acagcaccag caaccacggt aataactgct tgccttgcca
    10321 tttctgcagc gcggcgcgac agccataacc accaatcaga aacgcgatag tttgcgggac
    10381 ataacttaaa ccaataactg ccgggctgta gcccatttca ctaaggatga acggtgaacc
    10441 ggtcagccat gcaaaaaaac tggctgaaca ggccgcgtat atcagcacat tgccgcgata
    10501 ggttttagaa cgtagcaggt cggtaaaggt cagaccatcc tgactattgt tacgggcctt
    10561 cgtcgtgggt ttgagccaga aaataggcag aatcagcacc acggtaatgg caaaaagggt
    10621 ggcgaaaatc gcctgccagg aaaaatggac cagcagccag cttcctaaca gaggagccag
    10681 ggccggagat agacccacca gcggcatgat ggtcgcaaaa attcggttaa ctttctgtga
    10741 aggataataa tccgtcacta acgcttgcca gataaccgcc gcggcgcaga cacccacagc
    10801 ctgtacaaaa cgcaatacca gcagcatagc ggcgttttct acccacagca tccccagact
    10861 acctaacgca aaaattgtca ggccgattaa taataccggt ttacgaccat aacgatcgga
    10921 gagcggcccc cacagaagct gggctgcggc aaaaccggca aggaacagac taaggctggc
    10981 actgacagca gacgcaggcg tttgcaggtc ggcctgtatg gcggcgaaag caggcagata
    11041 catatcggtt gccagaaaac cgagtacgct caaacccgcc agccagacta aaaatctttt
    11101 cccaggttgc atgtatattc tctaaatctc tcaattaaac accgccgcaa agtgtaggga
    11161 gtgaaattag tgttgtgaaa cggtaatatt tgatgactgg tttcaaaaat tttgcaggca
    11221 gactatgtgg tcagaatatt cactcgaagt tgttgatgcg gtagcgcgta atggtagttt
    11281 tagcgctgcg gcacaggagc tgcatcgcgt tccttctgcg gtcagctata ccgtgcgtca
    11341 gctggaagag tggctggcgg tgccgctgtt tgaacgacgg caccgtgatg tggagctgac
    11401 cgctgccgga gcgtggtttc tcaaagaagg gcgctctgtt gtcaaaaaaa tgcagatcac
    11461 ccgccagcaa tgtcagcaga tagcgaacgg ctggcgcggt cagttagcta tcgcagtgga
    11521 taatattgtc aggccagaac gtacacggca gatgatcgtt gatttttatc gccattttga
    11581 tgatgtcgaa cttcttgtct ttcaggaagt gttcaacggt gtctgggatg cgctttccga
    11641 cgggcgcgtg gaactggcga ttggcgcaac acgcgcgatt ccggtaggcg gtcgttatgc
    11701 cttccgggat atggggatgc taagctggag ttgcgttgtt gccagccatc acccgctggc
    11761 gttgatggat ggcccgttca gcgatgatac gttgcgcaac tggccgtcgt tggtgcgcga
    11821 agacacctcg cgaacgctac ctaagcgtat tacctggttg ctggataacc aaaaaagagt
    11881 tgtcgtgcct gactgggaat catcggcaac ctgtatctcg gcaggattat gcatagggat
    11941 ggtgccaaca catttcgcca aaccgtggct taatgagggg aagtgggtag cattagaact
    12001 ggaaaacccc tttccggatt cggcatgttg tctgacatgg cagcaaaatg atatgtcgcc
    12061 tgcgttaacc tggttgcttg agtatctcgg cgatagtgag acgctgaata aggagtggct
    12121 gcgggagccg gaagagactc ccgcaacggg tgattaacga cgatagtcgc ggaacgggcc
    12181 gtcagccacg gagcggcgtt caatcaagcg cggatgcact tcaatggact gcggttcttc
    12241 acgtttgttg actatacgat ccaacaacat gttgaacgct gtttcaccca acgaatcttt
    12301 tggctgatgg attgttgtca gcgccggcgt aaaatagcgg gcgttgcgca cgttatcata
    12361 accgatcagc gaaacatcct gcgggacgcg cagccccatt tcatcagcag cacaaagtgc
    12421 gcccattgcc atgatatcgc caccacagaa gacggcagta gggcgatgcg gctgcgacag
    12481 gatttgctgc atggcgcgat aaccggattc aggttcaaag tcaccctgca caatccagct
    12541 ttccggcacc ttgatcattg cttcttccat cgccttcata aaaccggcaa ggcggcctgc
    12601 gccagtgtta cgttccagcg ggccggggat aacgccgatt tcgcggtgac cgcgttcaat
    12661 cagataacgc ccggccatgt agccgccttc gaacgcgtta tcaatgaccg catcggtgaa
    12721 gtcagctttt gcttcacccc agtccatcac caccattggg atatggcgat actcttccag
    12781 catcgccagc aacggctctg ggtactcaga acacatcacc agcagaccat cgacgcgttt
    12841 ttgcgccatc atcgacagat aagcccgctg tttctcaaga ttgttccacg cattacccag
    12901 aatcagggtg taacctttct ggaagcaatt tttttcaact gcttcaatga tctcggcaaa
    12961 ataggccgct tcgctgctgg tcgctagcaa accgatagac ttggtgtggt taaccttcag
    13021 gctacgcgcc accgcgctag gggagtagtg taattctttg atcgctgccc acacggcgtt
    13081 gcgcgtttct tcagcgacga aacgtgtttt gttgatcacg tgtgacacag ttgtagtgga
    13141 aacgtttgct cgtttcgcta catcttttat tgttgccatt ccatttcact ccagacccta
    13201 acttcagctc ctgcaaaatc gcaaggtaaa cgtttgcctt tacacacctt acgcacaaca
    13261 aaagtgttgc ggtacgccgg aaaaacgaca cggagcgtca ggaaggggtc aatggccagt
    13321 acgcaaatag tgtaagcgtg gaattttggc cgatcttgac gaaaagggga agtggtaaaa
    13381 acacttatca ttataaatga cgggagattt tttcatcaca gtgtgtaaaa atgcgtaata
    13441 tcactttttg tgtggtaata aaaaaggaga aaacttgatg agcaccgacc ttaaattttc
    13501 actggtaaca acgattatcg tcctcggttt gatcgtggcc gtgggtttga ctgccgcgct
    13561 gcactgattt cctctcgagg gagcgcgttc tccctcgcat ctccttcctg tttgttagca
    13621 aaatggcaaa agtgttttct ctttttgtta ttttcatttt tttgtgattg ctgtcatgct
    13681 ttcgccactt taatgaatat cgcgtaatga cgcgaagagg tggagataag gcatgaaaat
    13741 taactatccg ttgctggcgc tggcgattgg cgcgtttggt atcgggacaa cggagttctc
    13801 gccaatgggc ttgttgcccg tcattgcgcg cggtgtggat gtctcgattc ccgctgccgg
    13861 aatgttaatc agtgcctatg cagttggcgt aatggttggc gcgccgctga tgacgcttct
    13921 actttctcat cgtgcccgcc gcagtgcgtt gattttcctg atggcaattt tcacgctcgg
    13981 caacgtactt tccgccatcg cgccggatta tatgaccctg atgctttcac gcattttgac
    14041 cagcctgaat cacggagcat tttttggttt gggttcagtc gtggccgcaa gcgtggtgcc
    14101 aaaacataaa caggccagcg cagttgccac tatgtttatg gggttaaccc tggcaaatat
    14161 cggtggcgtg ccggcggcga cctggttggg tgaaaccatc ggctggcgga tgtcatttct
    14221 ggcaacggcg gggctgggag tgatttcaat ggtaagtctg ttcttctcat tacctaaagg
    14281 tggtgcaggg gcacgacctg aagtgaaaaa agagctggcg gtattaatgc gtccgcaggt
    14341 gctgtctgca ttgctgacga cggtactggg agctggtgca atgtttactc tctacaccta
    14401 tatctctccg gtactgcaaa gtattaccca cgcaacaccg gtgttcgtca cggcaatgct
    14461 ggtgctgatt ggtgtcggat tctctatcgg taactatctc ggcggcaaac tggcagatcg
    14521 ttcagttaac ggcacgttga aaggcttttt gttgctgctg atggtgatta tgctggcaat
    14581 cccgttcctg gcccgcaata agttcggcgc agctattagc atggcggtgt ggggcgctgc
    14641 aacctttgcg gtcgtaccgc cgttacagat gcgcgtgatg cgtgtcgcca gtgaagcgcc
    14701 aggtctgtct tcatcagtca atattggtgc ctttaatctt ggaaatgcgc tgggagcagc
    14761 tgctggtggt gcggtaattt ccgctgggct gggatacagc tttgtgccgg tgatgggggc
    14821 gattgtcgcg ggactggcat tattgctggt gtttatgtca gccagaaaac aacctgaaac
    14881 agtttgcgtt gctaacagct aattaaacaa aaacgcagaa ggataacctt ctgcgttaca
    14941 gttgctgaca acgtgcgatt tatcttgccg aatgcgctgt taacgcctca tccgcgttgg
    15001 catggtaagc gaagcgcatc aggcaatact gcatttgcca tcagttatta tgcagcgaga
    15061 tttttcgcta cgaattccca gttcaccagc gcccagaagt gctcaagata gccaggacgt
    15121 gcattgcgat agtcgatgta atatgcgtgt tcccagacat caacggttag cagcggagtc
    15181 gcatcggtgg tcagcggagt acccgcgtta gaggttgaaa cgatagccag tttgccatcg
    15241 ctgtttttca ccagccaggt ccagccagaa ccaaagtttt tgatcgctgc atcagtaaac
    15301 tgagctttga aatcggcaaa gctgccaaaa gatgcggcga tagcttcagc gacttttcca
    15361 gtcggttcgc caccggcgtt cggtgccagg cagttccagt agaaagtatg gttccagacc
    15421 tgagctgcgt tgttgaatac gccaccttca gagctgcgaa taatctcttc cagtgattta
    15481 ccttcaaacg cggtaccttt aatcaggttg ttcaggttag tgacataagt ctgatgatgc
    15541 ttgccgtagt gatactcgat agtttccgca gaaatgtgcg gtgccagagc atctttagca
    15601 tatggtagtg caggtaattc gaatgacatt gctactctcc tttattatta atttgcatac
    15661 gtacaatagc cttattgtgc gtatggatag ggtagcaaat taaaaggtga gacaaaagat
    15721 gaacttaccc tgttgccgta acaacagggt aaagttttag cgaagtgttt ttggggtcat
    15781 tacccggcga gcgccaacat agtggcgctg ccagtagtct tcactgagag aagtgatttg
    15841 aatttcctga cctgtgcgcg gtgactgaat aaatttgccg ttgccgacat acacgccgac
    15901 atgatcggct gtaccgcgtc cctgagtacg gaaaaagacc aggtcgccgt ttttcagttc
    15961 actacgttcg attggcgctg catcgcgcag gtgatacatt tcattcgccg tacgcggaat
    16021 acgaattttc accaaatctt tataagcgta ataaaccagg ccgctgcaat caaaaccggt
    16081 acgcggtgag ctgccacccc aacgatatgg cttaccaatt tgctgcatca gtttattcat
    16141 tgccactttt gtggcttttt gcaccttcgc tttgtgtgcg tcggcaaggg ttcctgacgc
    16201 tgcatttttg actttcacac aatgcgattt atagccctta cgtttggtac acttttcggt
    16261 aaagctggct gaggccgttt tattgacggt atgtttgctg cgagttttgg cgtttttaga
    16321 agcggtaatg gaagattttt taacggaaga agcggttttg cttttggtcg tacttttact
    16381 ggcggctttt ttcgctgttt tctgggtctt tttggtggtt gccgtgcttt tctttttatc
    16441 tgcttttttg gtgatatgag tggtagcaga actctccctg gcttgctttg aagcatgggc
    16501 cataggcgtt aaaggcaggg tggtaaaaag taaagcacag agcgtgatcg aaatacggtt
    16561 tatccgcgcc actgagcgtc cctctgttaa ggcaggtcta tcatgcctgc gtctgattta
    16621 caaaacttga tgattctaat gcatagaaga agagaaagtt aatagctttt attgttaaaa
    16681 aatacgtttc gttggcgagc gcaaaaaaat gcagcagtga aagctaaaaa tggtcataaa
    16741 tacgccagtt tgccgtgcat cagggaagaa acgaatattt gaaatgcaac aaatagagca
    16801 aagggataaa atagcaaaag cgtgatgaaa atgttatttt ccgatgccgc actgacccgc
    16861 tacaatgtca gattactgcc gtaaagaagt ttgaggaagc aagacaatga gcaccactat
    16921 cgaaaaaatc caacgccaga ttgctgaaaa cccgatcctg ctgtacatga aaggttcacc
    16981 gaaactgccg agctgcggtt tctctgccca ggcagtccag gcgcttgccg catgtggcga
    17041 acgttttgcc tatgttgata ttctgcagaa tccggacatt cgtgcggagc tgccgaaata
    17101 tgctaactgg ccgaccttcc cgcaactgtg ggttgacggc gagctggtag gcggttgtga
    17161 tatcgtgatc gaaatgtatc agcgtggcga actgcagcag ctgatcaaag aaactgccgc
    17221 taaatacaag tctgaagagc cggacgcgga ataatccgtc ctgcaatact gacggatgcg
    17281 aacgggcatc cgtcagtcct ttctctatcc ccaatccagc ccttgtggcg aacttgaaaa
    17341 tcccgcttca cgcagcagcg taaacatcgg cgtttgccag accggtagat cattcacttc
    17401 tgttagcgta aagcgcagcc gtggttcgcg acgcagtgcg gtagtcagcg cgtggaaaac
    17461 ctctggggcg agtaattcct ctttttcctg ccagaccagc atttttttgc caccttgcgc
    17521 cagatagagt aacaatttgc cgccagaaac gaccaccagc gcacccgccc gacgcgttgg
    17581 taccagcgtt gccggatgtg caggccaggg cagaagattt ccccacacat tagccggatc
    17641 gttggcggag agcgccactg gtgtatagtg gcgcgtttgc gcggcttgtg tcgccaggtc
    17701 gcgcaagcgg tcaatagtca gacgttcagc gaattgcgcg ccacccagac cttctacaaa
    17761 acgacctcgc ataattcgcc cggaatcttc catacttcga caaagcgttt gcatcgatgg
    17821 aaacccgcca gggatatttt cggctatcac cgcctgacga ctgatgatgc cgtagcggtc
    17881 gagcatattt tccgccagcg ccagcatcct ttcggtatcg tttagtggct ccacctgcaa
    17941 taacgaccag cgtccagcca gatttggtgt gttgtaagat acccgcggcg agacgggttg
    18001 tgcatagaca ggacgcccac gacgagcccg gtgactgcgg cgagttgagg tgcgtgcgtt
    18061 ggaactgctg cgggtgaggg cgcgtaacgg tgcccaaata tcgctggtga tgacgccttg
    18121 ccagaccagc gcccataacg cctcttgcag tgccgaggga tcaaccgatt cgccgatttt
    18181 gtcgcgtatc cgttggctga tttgttgtgc aaaccaggct cctccgtcag ccagaacagc
    18241 gactatcgct tgttgcagcg ccgaacgatt cgcctgatcc gcttcggcgg gagtgaacga
    18301 ttctgcagca tattcctgta gatgcagtgc caccaggccg tcatcttcac ccagtttttt
    18361 ttgccccgac cagataaccg caccggttgc cagtaattca tcgagcactt ctggcgaata
    18421 gtcgtgtacg cgggcaggca agatctggct ttcccagagt gaggcgggta agccgactcc
    18481 ggcaagctgt tcgatcaccc gcatcacgcc atccaccccc tcataaacgc ctggcgatgt
    18541 tgaggcaaag agcgccgggc taccatcggt ggcgggtaat acgccctgac gttccagcag
    18601 caatcgcgca taggtcgtgg ctgcaacggg acgcgtcgct tctctggcgg cttgcagcga
    18661 gcgcaaacgc agacgacgaa atacttcatc gctcacccag atgtcttgtt gcagattcat
    18721 caccagaccc tgttcgcgca gttgctgaag ctgctcttcg acaatggcaa taccgagact
    18781 aaactcatga gccagttgtt cagccgtgac cagagcatga gcccggagat agcgtagaaa
    18841 gaggtcgcga agcgggtaac tgactctatg caaataaatc tctggcagtg actctggtaa
    18901 tcgtacgccg agggcatcgc gcagcctggc ggcatcatcc atacaggcaa gacgctcctg
    18961 tccactaatc atcgtcggga agatgcgttt tactgtaaga agattttcca gatacgaggc
    19021 aatctcttca ctgctgcctg tatgccgttg cgccaggtct tcaacggtca ttggccccag
    19081 ttcgcgcagc aggtcgaaca atccttcttc acctttcgct cttctgccag gagccagtcg
    19141 ttgcaactct tcttccacct ggcgaatgac ctgcgggtcg agtaattccc ccggatcgac
    19201 ctgtccgagt agattgcgca gtaactcgct gtccagcgac agtacggatg cccggcgttc
    19261 tgccagcggg gcgtcgctct ggtacataaa ttccgcgaca tagccgaaca ataaacttgt
    19321 ggcgaaaggc gagggcgtag tagtcgttac atcggatatt tgaatttcgc ctccgttcag
    19381 gcgacgcatc aaacgttcca gtgcgggaag atcataaaca tcttgcagac attcgcgtag
    19441 ggtttcgaga atggccggaa aatccggata tccctgagcg atttccagca actgactggc
    19501 gcgcagccgt tgttgccaaa gcggggtgcg atggcccggc gtgcggcccg gcattaataa
    19561 tgcccgcgcg gcgcattcgc gaaaacgggc ggcgaaaagt gccgaactgc ctaccgcctc
    19621 gcggacaatt tgcagcaact tttctggttc aaacaaaaaa atcgcggcgt cgggcaattt
    19681 accatcagtg tcaggaatgc gcgcaacaat gccgtcatca ctggcgacca cagacgcgtc
    19741 agcgccccaa agcgcatgta ttcgcccggc aatcgccagc gcccagggtt catgcacccg
    19801 tcttccatag ggagagtgca aaataatacg ccagtcgcca atttcatcac ggcaccgttc
    19861 gaggaccaga tgacgactcc ccggaacaat acccgtcgcg ttgcgctgct cgtcaatcaa
    19921 cccctgaata ttggcgatcg tattttcttc agccagccac gggggaatag tcccggaaaa
    19981 gaacgctcca tccgccagca aatgaagaaa atcgccgatc atctcgccta attcagccgg
    20041 acgtccgtta ccttcaccac gccagaaggg gagccgggca gaacgacccg gagcaggagt
    20101 cacaatcacc tgatcgcggg tgatttgctg gatccgccat gaggttgcgc cgagagtgat
    20161 aatatcgttc acccgcgact catataccat ctcctcatcc agttcaccca cccgtcgcga
    20221 accggcctgt tcttcccctt cgggtaataa cacgctatac atgccacgat ccggaatggt
    20281 gccgccgctg gtaaccgcca acagctgagc gccaggtcgg gcggtcaata tcccggtctc
    20341 cctgttccag accagtttgg ggcgaaaagc agaaaaatcg ccagagggat agcgtccgga
    20401 aagcatatcc agcgtggcgt caaaaacacg tcttggcaga tctttccacg gtgcggcacg
    20461 gcgtacgcgg gagtaccatt cgtctacctg taatgcatcc atcgccgcgg cggcaacggt
    20521 gtgctgcgca aggacgtcga gtggattatg cggaggtgtc aggttttcca gcctgcctgc
    20581 gaacatacac tctacaatga ctgcggaatc gactaagtca cgcctggttc gggggaaaaa
    20641 aagccctttg gagatacctc ctatctggtg tccggcacgt ccaatgcgtt gtaacccact
    20701 ggcaacagaa agcggcgttg ccacctgaat caccagctcc acagcgccca tatcaatccc
    20761 cagttcgaga ctggaagttg cgaccacgca gcgtaactca cccgatttca gcgcctgctc
    20821 ggtgattgct cgttgttctt tagagacgga gccgtggtgt gagcgggcaa taaaaacgtc
    20881 gctactttgt acacggttag aggttgcgcc agaggtcgac tcgaaatggg ccgcatcagc
    20941 ggcgatagac ggggaacgct gtaagcgtgc ggcgtaaagc tcatttaatc gtgccgtcag
    21001 tttttccgcc agcccccgcg aattagtaaa gacaatggtc gagcgatggc gcaacacttc
    21061 atcaaggata cccgtttcaa tatatggcca gatggagcct tcccggccgg catggctgtc
    21121 ttcgccagtg ccgctggcga ccgatgagac atcatccata ttggcgactg gtacgacaat
    21181 ccgtatctgc ggatgacgca ttgcgggcgg gttgactacc gtaaccgggc gatcgccacc
    21241 aagaaatgct gccacatcgc tggctgagcg cacagtggca gaaaggccaa ttcgctgtgc
    21301 tgaggtgtgg agcagcgcat cgagccgctc cagacttaac gccagatgcg caccacgttt
    21361 acttcccgct accgcgtgga cttcatcaat aattaccgtt tcgacgccgc gtagcgtttc
    21421 gcgcgcgcgg gaggtcagca tcagatagag tgattcgggt gtggtgatca gaatatccgg
    21481 cggattacgg gtgagtttgc tgcgttcctg tgcaggcgta tcgccagtac ggatccccac
    21541 gcgaagattg acttccgttt cgccgcgccg ccgccgttca tcggcaatac ccttcaacgg
    21601 gatctgcaag ttgcgctgaa cgtcggtgcc cagggctttt atcggtgaaa tatagaggat
    21661 gcgtgaggtt ttacgtttat gcgcctcgcg ggtatcttcg ccgccttcgc ggaagagtcg
    21721 atcgagggcg tagagaaatg ctgccagcgt tttcccggag ccagtcggtg caatcaccag
    21781 cgcatgttcg cctcgcgccg ccacatgcca ggtttgcgac tggacagcgg tcggctgttt
    21841 aaaggcgcga agaaaccagt cgcgggtcgc cggtgaaaac acgtccggca ggggcgatga
    21901 agggtttgga ttatctgcca ttaacgctat tccccgtgaa tatcatgcat gatgcgtgga
    21961 atagcctcac tatagatgcc tgaatcattg catgtcatca ggcatcgact cgattacacc
    22021 tcttcggcgg caggtagcgg ccagcctccc agacgtttcc agcggttgac gatttcacaa
    22081 aacagcaccg cagtgcgttc ggtgtcgtac agcgcggagt gcgcctgggt gctgtcgaag
    22141 tccatgccag cggtctggca agcctttgac aatacggttt gtccgagtgc cagcccggcc
    22201 agtgcagcag tgtcaaaagt ggcgaaaggg tggaacgggt tacgtttcag tgaggcgcgt
    22261 tctgcggcgg ccatcataaa gctgtgatcg aaattggcgt tgtgcgccac cataatggcg
    22321 cggttacagc cgctcgcttt aataccttta cgtacaactt taaaaatttc gtgcagcgcc
    22381 tcgtattcgc tgaccgcgcc gcgatcggga tcgttcgggt caatgccgtt gaaagcaagg
    22441 gcttctggtt gcaaatttgc gccgacaaat ggttcgacgt ggaaatgtaa tgtggtgtcc
    22501 ggcatcagcc agccttgttc atccattttc agggtgatgg cggcaatctc aagcagcgca
    22561 tcggttttgg cgttaaatcc agctgtttca acatcgatca caacaggata aaagccacga
    22621 aaacggtcgc acagaccggt aagttgagcg ttatcggaca tctgattctc ttaatacgga
    22681 taaattgcag cgcgcattat gacaaatatt acttgtcgat gcagtaaaga tgcgggcgcg
    22741 atgagttcgc gcccggcagg agattagttg cccagaccgc gaccggcgtc tttctcttcg
    22801 attaactcaa ttttgtaacc gtccggatct tccacaaacg cgataaccgt agtaccgcct
    22861 tttaccggac ccgcttcacg ggtcacgtta cccccgtttt gacggatttt ttcgcacgct
    22921 tcagcggcat tatctacgct aagcgcgatg tgaccataag cagtgccgag ttcgtattta
    22981 tccacgcccc agttgtaggt cagttcaatc accgcttctt cggtttccgg gccgtagcca
    23041 acaaacgcca gtgagtattt gtattccggg ttttcgctgg tacgcagcag tttcatgccc
    23101 agcactttgg tataaaaatc gatggagcgt tgcagatcgc caacgcgcag catggtatga
    23161 agaagacgca tctttttatc ctcaaaatgg ctaacagatt caaaatgttg ttttagtata
    23221 gcggcggctt tacgccgctc gcaatgttgg attacaacgt cgggtaatca gtgtagcctt
    23281 ccgcaccgcc accatagaaa ctttcagcac gctgtgggtt aagctcagct ttgcgctgca
    23341 agcgggcgac cagatccggg ttcgcaatcc agtcacgacc aaatgccacc gcatcaatta
    23401 accctttgcc gatcagcgtt tcagcttttt ctactgtgta tgcacctgcg ccgataatcg
    23461 gaccgtggaa acgggcgcgt actttttcgc ggaacgcatc agtatacggt tcaccccccg
    23521 cccaatctgg ttctgacata tgcagataag caatgccgcg tttacccagt tgttcaatca
    23581 gatacagtgc atcggcttct tcattcgggc cgttatccgt gttctggaaa gtaccgattg
    23641 gcgaaacgcg aatgccaatg cgatcggcac cccattcttc aatcccggca tcgaccactt
    23701 ccagtaccaa acgtgcgcga ttttccacgc tgccgccgta ctgatcggta cgatggtttg
    23761 aagaaggaga aaggaactga tgcagcaaat aaccatgagc agagtggagc tctaccagat
    23821 caaaaccggc ttcacgcgcg ttagcaatag cctgacggaa atcattgacg atacttggaa
    23881 tctcttccag ttcaagcgca cgcggcatgg atgtttcaac acggatcgcc tgaccatttt
    23941 catcgcgcag agaagtacgt gttcccgcgc taagtgctga aggcgctact ggtgcctggc
    24001 cgccaggttg caggctggcg tgagaaatgc gtccggtgtg ccacagctgt acggccatat
    24061 gcccattttc agcatgaacg ccagcggtaa tttttttcca tgcggcaatt tgttccggac
    24121 tatggatgcc aggtgcacca gcataccctt ttgcctgggc agaaatttgc gtggcttcgc
    24181 taataatcaa accggcactg gcacgttggc gatagtattc cgccatcaac ggggtaggga
    24241 tgtcacccgg ttcaatactg cgcagacgcg tcagcggtgc cataaaaata cggtttgccg
    24301 cagttattgc gcccactttc agtggggaat acagtttttc agatgacata aaggctcccg
    24361 gtcgtctggt gataaaaaat gcctgctaaa cggcaggcgt cgcaataatg gtttttacat
    24421 gtgccagcgc gttttccagt ggctcggaat tgcgcgaaat tttggcctgc agattcgcac
    24481 ccagccatag tgcgtaaagc acttgtgcct gttgcagcgg ttcgccacaa aaggttaaac
    24541 aatggctatc acggccattt tccagagcct gcgagagcag ggcgatcacg ccgcgagcgc
    24601 ctttatccat cgcgctacgc atatcttctg acagatcgca cacttcggca gagagtttta
    24661 ctgtcaggca accactgatg gttccatgtt ggcaaaactg gttcagtgtt tgctggtaat
    24721 aagccagtat gcggtcgcgg tagttacctt cgccggattg cagcaactca gtcagtcgtt
    24781 ggtgatatgt ggcgtaatgg cgctcaagca tggcaacgcc aaacgcttct ttagagcgaa
    24841 agtagtgata gaaggaccct ttcggcactt cagcggtttt tagtaattcg cttagcccca
    24901 tcccggtgaa tccacgttgc aggcaaagtt gctcgcccgt cgccaggaga tgttcgcgag
    24961 tatcatgttc ggtgtgtttg ttcatgcgtt ggagtgtagt agaccagtcg gtctagtgca
    25021 agcaaagttg ctactttggg cattaacagc ttcaataatg gtaataatct tatttgagga
    25081 gggcgaagtg gcggagcaat tagagttctt tcccgtccag agcccgtgcc ggggaatttg
    25141 ccagtctgac gaacgcgggt tttgtcgcgg ctgttttcga agccgggatg agcgttttaa
    25201 ctggaataaa atgagcgatg gcgaaaaaca agaggtgctg cgcctttgtc ggcagcgatt
    25261 aatgcgcaaa ttacgtgcaa ataagccagc gtcatcagac gaaccggagc aaccatcact
    25321 cttttgagcc taatcttgcg tatactcctc tcatatctga taagaggaag cgattatggt
    25381 tcagcgtatt actattgcgc cgcaaggccc ggagttttcc cgttttgtga tgggctactg
    25441 gcgattgatg gactggaaca tgtccgcccg ccagctggtc agttttattg aagagcatct
    25501 ggatctcggc gtgaccaccg tggaccatgc tgatatttat ggtggctatc agtgcgaagc
    25561 ggcgtttggc gaggcgctga aactggcacc tcacctgcgt gagcggatgg aaatcgtcag
    25621 taaatgcggt atcgcgacga ccgcgcgtga agaaaacgtc attggtcatt acatcactga
    25681 ccgcgatcac atcattaaga gcgccgaaca gtcgctaatt aatcttgcga ccgatcatct
    25741 ggatctgctg ttaatccacc gcccggaccc gttaatggat gccgatgaag tggcggacgc
    25801 gttcaaacat ctgcatcaga gcggcaaagt gcgtcatttt ggcgtatcca acttcacgcc
    25861 tgcgcaattt gccctgttgc aatcacgtct gccgtttacc cttgccacta atcaggtgga
    25921 aatatccccg gtacatcagc cgttactgct ggatggcacg ctcgaccaac tgcaacaact
    25981 gcgtgttcgt ccgatggcgt ggtcctgcct tggtggtggt cgtctgttta atgatgatta
    26041 tttccagccg ctgcgtgatg aactggctgt ggtggcggag gagttaaacg cgggctcgat
    26101 tgaacaggtg gtttacgcct gggtattacg tttaccgtcg cagccgctgc caattatcgg
    26161 ctgaggtaaa attgagcgcg tacgggcagc tgtcgaagca gaaacactga aaatgacccg
    26221 tcaacaatgg tttcgtatcc gtaaagcggc actggggtac gacgtaccgt aagcactttt
    26281 aggaatagcc gccgttcaaa aatgtgtcac tggtttacac ttattcagga atgcacaatg
    26341 aacggaggtc ctatgaaacg ttttagtctg gccattctgg cgctggttgt tgcgaccggc
    26401 gcacaagctg ccagtgaaaa agtcgagatg aacctcgtca cgtcgctagg ggttgggcag
    26461 tcaattggta gcgtcaccat tactgaaacc gataaaggtc tggagttttc gcccgatctg
    26521 aaagcattac ctcccggtga acatggcttc catattcatg ccaaaggaag ctgccagcca
    26581 gccaccaaag atggcaaagc cagcgccgcg gaatccgcag gcgggcatct tgatccacaa
    26641 aataccggta aacatgaagg gccagaaggt gccgggcatt taggcgatct gcctgcactg
    26701 gtcgtcaata atgacggcaa agctaccgat gccgtcatcg cgcctcgtct gaaatcactg
    26761 gatgaaatca aagacaaagc gctgatggtc cacgttggcg gcgataatat gtccgatcaa
    26821 cctaaaccgc tgggcggtgg cggtgaacgc tatgcctgtg gtgtaattaa gtaattaaga
    26881 ggccagcgta ccttgcggtg gtgcttgctc aagttgcgaa agcgagcagt acagccgcca
    26941 gacaattgcc gccagctcac gggcggcagg ttgatgatgg cgggcaaggc tgtcgcaaat
    27001 ccgctgtaat tcttcaagtg tggccgccag tgatttttgc tgaacgccac gctcactcat
    27061 cactccccgc aacagtgaaa tacagttatc ccgtactcgc gataacggat cggaacgcga
    27121 ttcccagtcg cgtaattgcc agacaacatg agaacagttc agcagcacta taccccagcg
    27181 taataaccaa cggcgagcca gcgcatcctg gctgttactc aactgactga cgtgatgata
    27241 agtgagcgat tcaaattcgc tttcactcag tgttggatgg cggcttagtt gatcgacaaa
    27301 atcccggcgc agcgcgcgaa tatggcggcg gcttttgcga gcatccgatc ctggacgcag
    27361 aatggcgaac gctaaccacg ccaacgcgac gccaacgatt tttgccagat tatcgttaag
    27421 aaaatcagca aaattataca ccgggggatt agtgacggcg ataaaagaac ccataaaaac
    27481 aatcagttgc ccccacaatg cggcaaattt gggcatctgc aatttaagaa gctgcattgt
    27541 cgccagcagt ggaaagagaa acagtaaaaa ttgccacaga tcgctaatct ggaccatcag
    27601 accaaatttg accacaaagc tgaatagcga aagtaacacc agcgtgcgca tcagcagtga
    27661 caacgactta aacggtgctg cgacggcgga gtagagtacg cagctaatcg ctgccagcgt
    27721 taatgcattg gcaccggcat cccattgcga agcaatactc catgcgccaa tcatcatcaa
    27781 cgtacaaaat gtacgcagtc cgctccacat agcttcggcg ttatcggtat gacgagccag
    27841 gccggatgtc cgtgggagtc tggcatgatc atctacatcg ctttgcagac gatgtaattc
    27901 ctgactactt tgcagataaa ggcggcaaaa ataacgtagt cgctgccaga aggcgacgtg
    27961 ccgatagtcg gcgacgttgg tcgggcgtag cggggcgata atacgtgcga cggtgtaaac
    28021 atctgtttgc gaactggcga gcgccgtcag caactgttcg agaatttctc gcgtggcacc
    28081 tggcggtgag ggccagttga gcaacatacg tcgcaggctg gagatgacac tggtcatacg
    28141 taattgctgg tggagcagcg cattaaggcg cgcgttttgc tggcgaaaac gatagtggct
    28201 ccagaaagcc tggatacgca gcaaattcat ggtcagtatc tgcccaatca ccccttcatg
    28261 tgctgcacga atggcatcgg ttgtttcagg ctgccagagt aaactggcat gttccagtaa
    28321 tcgggcgtgc atgtttttca atgcggttaa aagggctgta gcatcggaac tgctaggcag
    28381 gatcatcatc atcatgccgc cgcacaaaat accgacaatc acctcgcaaa cgcgcgcctg
    28441 agcgatatcc cacagctggc tggcctcagt aatattaacc atcggaaagg cgatgatggc
    28501 agccgtgtag cccgccagtt gaaatgcata cgcgacgtta ttcgtgaagt gcgcacaggc
    28561 ccaggtacaa aagccaagcc acgccgacat gctcaataga aaaaaccacg gctcattgag
    28621 cgtatgcccg gcaagaagca gtgccgcaat ggctcccagt aaactgccag cgatgcgtcc
    28681 gaggcttttg ctgatgacac cgccaacggt gggaaagcta accactgcag ccgaggtcat
    28741 cgcccaatag ggttcatcca gattcaaata ataggcaacc gtcagcgcca gacacatggc
    28801 aatggtattg cgtaacgcat aacgccattg cgccagcgtg gccctgaacc agggcaaatt
    28861 gcgcaaggac catgacgatg cgttcattat tgacctacgg caatgctaca ggtggtgccc
    28921 gacaccagcg tgacatcgcc tggaacttta tccagcgcaa agcggacggg aacgcgttgg
    28981 gcgagacgaa cccaggggac gttaggtttc acatccggaa taaggcttga gtcactttca
    29041 acgctctgat cataaatcgc ccgaccgata ctggaaacgt gaccctgtaa cgttttgtta
    29101 tcactataga gggtaatttg tgctggtgca ccttcgcgga tatggcgcaa cttggtttct
    29161 tcaaaataac caatgacgta aaacgagtgg ctgtcgacaa gggcaaacag cggtttcccg
    29221 gtgtcagcgt aatcgccgat gcgggtagtg agattagtca cccatccact caccggggcg
    29281 cgaatttccg tttgcgccag ttgccattgt gtctgtttca gcgtcgcctg tgcggcatca
    29341 acactggcct gcatcgcttt aacattgagg ttagcggtat cgagttcttc ggcagaaata
    29401 aaattttgtg agagatgacg acgacgattc gcttcgttat tggcttttgc gaggtcagat
    29461 tgcgctttcg ccagctgagc ctgtgcattc agctcggcga tctgaaaggg cgttttatcg
    29521 atcgtgagca gaagatcccc cgcgttaacc agctggttgt cttttatatt cagctcaaca
    29581 atacgtcccg acacctgtgg ggtgatagaa acctgttctg cgcgtatttt cccgtcgcgt
    29641 gtccacggag attgcatgta ataattccac agccaccatc cggcaagaac cgcaactacc
    29701 gctacaatga ttgttgagaa atacttaatt gttttaattg acatagttac cacgcaatca
    29761 gtattgccag agcaaggcaa acgcaaatcg caaacagcga taaatccatt aacaagggat
    29821 gccagatgtc accggcgtag atccagccac gaagcaggcg gtgtacgaca agccagatga
    29881 cgaatccaaa cgcgaaggct ttgaaaaacg gaggaaagta gacggacgca ccgaacacca
    29941 gatcttgtaa gggcaatcct gttgcattga gcataaactt cacaaagtag attcctttac
    30001 gaccgaatat attgcgttag attaataaat attctttaac tgcgaaaaat ttacgcgcaa
    30061 tttctgaaaa atatgtctga cggtaaccaa atgcagcaat acatttgttt tagcaataca
    30121 attgctgcac actattctaa aagccgcata atatcttagc aagctaatta taaggagatg
    30181 aaattggaat cgccactagg ttctgatctg gcacggttgg tgcgcatatg gcgtgctctg
    30241 atagaccatc gcctgaaacc gctggagtta acacaaaccc attgggttac gttacacaat
    30301 atccatcagt tacctccaga ccagtcgcaa attcaactgg caaaagcgat tggcatcgag
    30361 cagccatcac tggtccgtac tctggaccaa ctggaagaaa aagggttaat ttcgcgtcaa
    30421 acttgtgcca gcgatcgtcg ggctaaacgt attaaactga cggaaaaggc agagccgctg
    30481 atcagcgaaa tggaagctgt tattaacaaa acccgcgcgg aaattttaca tggcatctcc
    30541 gcagaggaac tggaacaact gattacgctc atcgcaaaac ttgagcataa tatcattgag
    30601 ttacaggcca aagggtgaaa tgaagggggc gtgtggtcag taacctgacc acacgcaaac
    30661 ttatttagcg cggagaaacg gtcacctgac tgccattgct ggccagtacg acacgttggc
    30721 ccggagagaa acgagtgttg ccttgtttct gtaccaccat gatggtatta ccatcgtctt
    30781 tacgaatttc cagctcgaca ccttgcgttt tgttcattgc actctgtacg ccctgaccag
    30841 ctacgccacc tgcaacagcg cctgctgcag tagccagaga acgcccggtt ccgccaccaa
    30901 cagtatttcc caggaaacca ccaagaacag caccgccaat tgcaccgata acgttggaat
    30961 catcaccgcc ctgaatctgt accggacgta cgttaacgat ggttccatag ctgacattct
    31021 gtacttgttt cgcttcagaa gcggtataaa catcccctga cagggtgtcg ttattaacac
    31081 aaccgacaag agacagacct accattgaaa caaccaatac gcgtttaatc attgaaaaat
    31141 ctcctgttca ccatgaaacg ccacgcaagc atccctcatg gtcaaagtat atggcatatg
    31201 tgaatccatg gttacatgtt ctaccaaata tgagcaaatc ataatgaaag taggatcaac
    31261 caattataaa caaaacattc atgatgatta ttgatttgaa gataatgttt tactaccaac
    31321 ggtaaaaatc ggtcattccg catggtattc acgaaaagtt tatgtttgag tgtgcgcaaa
    31381 cgtcagattg aaaaaggatg aactatgaaa tcgggccgct ttattggcgt tatgtcaggc
    31441 accagccttg atggtgttga tgttgtgttg gcgacaattg atgaacaccg ggtcgcacag
    31501 ctggcaagtt tgagctggcc gatcccggtg tctctgaaac aggctgtact ggatatttgc
    31561 cagggccagc agcttacact ttcgcagttt ggacagcttg atactcaact cgggcgactt
    31621 tttgctgatg cggtcaaggc cttgcttaag gaacaaaacc tgcaggcgag agatatagtt
    31681 gcgatcggct gtcacggtca aaccgtctgg catgaaccga cgggcgtggc accacacact
    31741 ttacagattg gcgataacaa tcaaattgtg gcacgcaccg gaattacggt tgtcggtgat
    31801 tttcgccgtc gcgatattgc cttgggagga caaggcgcac cgctggtacc tgcgttccat
    31861 catgccttgc tggctcaccc aaccgagcga cgaatggtgc tcaatattgg cggcatcgcc
    31921 aatctgtcac tgctcattcc tgggcagccg gttggcggct acgataccgg tcctggtaac
    31981 atgctgatgg atgcctggat ctggcgtcag gccggtaaac cttacgataa agatgccgag
    32041 tgggcacggg cgggtaaagt tattctccca ctgctgcaaa atatgctcag cgacccatat
    32101 ttctcgcaac ctgcaccgaa aagcaccgga cgcgaatact ttaactacgg ctggctggag
    32161 cgccatttac gccattttcc gggtgttgat ccccgtgatg ttcaggccac actggcagaa
    32221 ctcaccgccg tgaccatttc tgaacaagtt ttgttgagcg gtggctgcga acgattgatg
    32281 gtatgtggtg gaggtagtcg taatccgcta ctcatggcgc gtctggcggc attactgcca
    32341 ggcacagaag tcaccaccac cgatgccgtt ggcattagtg gcgatgacat ggaagcattg
    32401 gctttcgctt ggcttgcctg gcggacgctg gcgggattac caggaaatct gccttccgtc
    32461 actggcgcaa gccaggagac ggttctgggg gctattttcc ccgccaactc gtgatatcag
    32521 aattaactga attttttctt atcgtgtctg ccgttacact tggaagatta aggaggatat
    32581 ttgccctccg gaccaggaaa gtcttcggga tatgactatg aaaaaactgt taattatcat
    32641 tttgccggtc cttctctccg gctgtagcgc ctttaatcaa ctggttgagc gaatgcaaac
    32701 cgatacgctg gaataccagt gtgatgaaaa accgttgacg gtcaaactga ataatccgcg
    32761 ccaggaagtc agttttgttt acgataatca actactgcat ctcaaacagg gcctttcagc
    32821 ctctggcgcg cgttactccg acgggatcta tgttttctgg tcgaaaggtg aggaagcgac
    32881 tgtctataaa cgcgaccgca tcgtcttgaa taactgtcag ttacaaaatc cacagcgttg
    32941 agatttttcc aggggcggcg cacaatagcg tcacccacag acaatccgta aagaaaacca
    33001 tgtctgataa cgacgaattg cagcaaatcg cgcatctgcg ccgtgaatac accaaaggcg
    33061 ggttacgccg ccgcgatctt cccgccgatc cattaaccct ttttgaacgt tggctctctc
    33121 aggcttgtga agccaaactg gcggacccta ccgcgatggt ggtcgctacc gtggatgaac
    33181 atggtcagcc ttatcagcgc atcgttttac tcaaacatta cgacgaaaaa ggtatggtgt
    33241 tttacaccaa cctcggtagc cgtaaagcac atcaaattga aaataatccg cgcgttagcc
    33301 tgctgttccc gtggcatacc cttgaacgcc aggtgatggt gatcggtaaa gcagaacgac
    33361 tttcgactct cgaagtgatg aaatattttc atagccgccc gcgtgatagc cagattggtt
    33421 catgggtttc gaagcagtcc agtcgcattt ctgcccgcgg tatccttgaa agtaaattcc
    33481 tggagctgaa gcagaagttt caacaaggcg aagtgccatt gccgagcttt tggggcggtt
    33541 ttcgcgtcag ccttgaacag attgagttct ggcagggcgg tgagcatcgc ctgcatgacc
    33601 gctttttgta ccagcgtgaa aatgatgcgt ggaagattga tcgtcttgca ccctgaaaag
    33661 atgcaaaaat cttgctttaa tcgctggtac tcctgattct ggcactttat tctatgtctc
    33721 tttcgcatct ggcgaaaagt cgtgtaccgg caaaggtgca gtcgttatat acatggagat
    33781 tttgatggca agcagtaact tgattaaaca attgcaagag cgggggctgg tagcccaggt
    33841 gacggacgag gaagcgttag cagagcgact ggcgcaaggc ccgatcgcgc tctattgcgg
    33901 cttcgatcct accgctgaca gcttgcattt ggggcatctt gttccattgt tatgcctgaa
    33961 acgcttccag caggcgggcc acaagccggt tgcgctggta ggcggcgcga cgggtctgat
    34021 tggcgacccg agcttcaaag ctgccgagcg taagctgaac accgaagaaa ctgttcagga
    34081 gtgggtggac aaaatccgca agcaggttgc cccgttcctc gatttcgact gtggagaaaa
    34141 ctctgctatc gcggcgaaca actatgactg gttcggcaat atgaatgtgc tgaccttcct
    34201 gcgcgatatt ggcaaacact tctccgttaa ccagatgatc aacaaagaag cggttaagca
    34261 gcgtctcaac cgtgaagatc aggggatttc gttcactgag ttttcctaca acctgttgca
    34321 gggttatgac ttcgcctgtc tgaacaaaca gtacggtgtg gtgctgcaaa ttggtggttc
    34381 tgatcagtgg ggtaacatca cttctggtat cgacctgacc cgtcgtctgc atcagaatca
    34441 ggtgtttggc ctgaccgttc cgctgatcac caaagcagat ggcaccaaat ttggtaaaac
    34501 tgaaggcggc gcagtctggt tggatccgaa gaaaaccagc ccgtacaaat tctaccagtt
    34561 ctggatcaac actgcggatg ccgacgttta ccgcttcctg aagttcttca cctttatgag
    34621 cattgaagag atcaacgccc tggaagaaga agataaaaac agcggtaaag caccgcgcgc
    34681 ccagtatgta ctggcggagc aggtgactcg tctggttcac ggtgaagaag gtttacaggc
    34741 ggcaaaacgt attaccgaat gcctgttcag cggttctttg agtgcgctga gtgaagcgga
    34801 cttcgaacag ctggcgcagg acggcgtacc gatggttgag atggaaaagg gcgcagacct
    34861 gatgcaggcg ctggtcgatt ctgaactgca accttcccgt ggtcaggcgc gtaaaactat
    34921 cgcctccaat gccatcacca ttaacggtga aaaacagtcc gatcctgaat acttctttaa
    34981 agaagaagat cgtctgtttg gtcgttttac cttactgcgt cgcggcaaaa agaattactg
    35041 tctgatttgc tggaaataat gcattaagtg gaaaggggga gtgagaaatc actccccctg
    35101 gtttttatac agggaacatg atgaaaaata ttctcgctat ccagtctcac gttgtttatg
    35161 gtcatgcggg taacagtgcg gcagagtttc cgatgcgccg cctgggcgcg aacgtctggc
    35221 cgctgaacac cgttcaattt tctaatcaca cccaatacgg caaatggact ggctgcgtga
    35281 tgccgcccag ccatttaacc gaaattgtgc aaggcattgc cgccattgat aaattacaca
    35341 cctgtgatgc cgtattaagt ggctatctgg gatcggcgga gcagggtgaa catatcctcg
    35401 gtatcgtccg tcaggtgaaa gcagcgaatc cgcaggcgaa atatttttgc gatccggtaa
    35461 tgggtcatcc agaaaaaggc tgtatcgttg caccgggtgt cgcagagttt catgtgcggc
    35521 acggtttgcc tgccagcgat atcattgcgc cgaatcttgt tgagctggaa atactctgtg
    35581 agcatccggt aaataacgtc gaagaagcgg ttctggcagc gcgcgaactc attgcgcaag
    35641 ggccacaaat tgtattggtt aaacacctgg cgcgagctgg ctacagccgt gaccgttttg
    35701 aaatgttgct ggtcaccgcc gatgaagcct ggcatatcag ccgtccactg gtggattttg
    35761 gtatgcgcca gccggtaggt gttggtgatg tgacgagcgg tttactgctg gtgaaactgc
    35821 ttcagggggc aacgctgcag gaggccctgg aacatgtgac cgctgcagtc tacgaaatca
    35881 tggtgaccac caaagcaatg caggaatatg agttgcaagt ggttgctgct caggatcgta
    35941 ttgccaaacc agaacattac ttcagcgcaa caaagctctg aaatatctca tttcggccca
    36001 caacgaactg tgggccgacg gctattactt taagccttcc gctgacagcg cgtcttgtac
    36061 ttccggacgt tcagccatac gttgcataaa tgccgcaatg tgctctaacc cttccagatt
    36121 cagtttcacc gcgtatgccc agcgcagtac cgtaaacaga taggcatcag caattgtaaa
    36181 tctttgcccg cagatccagt gctcatcctt cagtgcctcg ttcacatatt gcagtttctt
    36241 ctccagctgc gcgcgaactg tcggtttgta ctcttccggt gtatcagggc gaaacagagg
    36301 tgtgaaacct ttatgcagct cggtggcgat gtaattcagc cattcgatgg ttttatagcg
    36361 ggaaatactg tttaccggtg ccagcaactg gcggtcgggg acgctgtcgg caagatactg
    36421 cataatcgct acaccttccg tcagcaaagt accgtcatcc agcagcaatg caggtacctg
    36481 tcccttaggg ttaacggcaa agtaatcgtc accgttttcg agacgttttt tcattaaatc
    36541 cacactgacg agcgtaaaat cctttccgct ctcacgcagg gtgatatggg aagcgagaga
    36601 gcaggcgccc ggtttgtaga acaatttcat cggtaactcc ttttgtctgt ggtttaaagt
    36661 atggtagtgc gttgctgaac aaaaaaaaag ccgctaacga aaagttagcg gcttgtaaaa
    36721 gagtttccct gaattttacg ctacggctgc tttcgccgct ttgtctgcag cgtcatcctg
    36781 cgtcatgcgg tgcagtttcg gcgcggtcag cagcatcagt actgcaataa cggcagtagc
    36841 gacaccaatc tgcaagaata cgcgaccata gacttccagt gacatcagcg gatcggtaac
    36901 gttatccggc acagccatca tacccgcaac ataaccacca atcaggtttg caccggcagt
    36961 ggtcaggaac cagctaccca taatgaagcc catcagacgc tgcggaacga gttgtgcaac
    37021 cattgccaga cccagaccag agatcatcag ttccccgata ctctgcaggc catagctcgc
    37081 gaccagccag cttacagaca cgatacctgc gtcagacgcg aatttcgcac ccagcggcag
    37141 aatcaggaac gcaccagaac acatcaccat accgattgca aacttggttg gcatcggcag
    37201 ggtatcgccc atcttgttat agatagcggc cagaatcggg ctaccaatga tgatccagaa
    37261 cgggttcagt gcctgatact gctcaggttc tacggccagg cccagaatgg tatgttcaac
    37321 gttacgaatc gcaaagaagt tcagtgacgt tggcatctgg ctgtacagca cgaagaagat
    37381 aatggcttcg agcatcagga tgaaggcaac gatcatttta cgacgcgcag cacctttcat
    37441 cgcgaaggct tctttaccga agataaccac gataccgaag gcaacaacgc ccagcgccat
    37501 acgcgcaact tcctgattgt gcagcagcca ggtggcgata gcgatcagtg ccacaacacc
    37561 aataatggtc agcagcaggt tacggtagtt gataggctcg aagtctggtt ttgaaccgta
    37621 ctgtttaacc cagcgttggc agaaggcgaa gttaacgata gtgatcagca ggcctacaac
    37681 gctcaacgca aacgcaacac tccagccgta tttcgcggcc agccacgggg tggcgatcat
    37741 agagaagaaa gagccgatgt tgacggacat gtagtacatg gtgaatgcac cgtccagacg
    37801 cgggtcgttt ttctcatagc atgtagaaag cagagaagac gggttagctt taaacaggcc
    37861 gttaccgacc gcaatagccg ccatacccat ataaacgata ccggcgtcgt gaccagacca
    37921 ggcaaccaga gcataaccaa tcgccagcac aatagcgccg agcataatta cgcgtttagt
    37981 acccagtacc ttgtcaccta accagccgcc gatagcgacc agaccataaa ccagggcact
    38041 aaaggaagag aaaagggtga ttgaatccgc ttcagacata cccagttgtt taaccaggta
    38101 aacagccata attccttgta ggccgtaata accaaaacgt tcccataact caatcgagaa
    38161 gatgagatag aacgccttcg gttgtttgaa agcgttcaaa ctgacgcttt cagttggttt
    38221 ttggtttgca gtggacactt ttacctcttt ttttacatcc catattaacg ggggtgcttt
    38281 ctcgcgttat gtctaaaaaa catacgcgca attgttatag tgggagggga aacggcaggt
    38341 aatgttcact atcttgcact ttcagacaag tcctttgtaa taatctgtta catataactg
    38401 aggcggggaa ttcaaccatt ccgggaaatg ttatccagcg ttaaattttt caaaaaacgt
    38461 aacggcagat tttttcactg cattgagagg ggggtgatgg cttgacggcg atatgttctg
    38521 ctatttagcg tttttcctag tgagatagtc cagtttctta aaaatagcca gtgtaatgtt
    38581 ttgtaggtca atatttgttg cgtgtgtaaa tttaaatgat tttaacctga ttcttgcaat
    38641 gtttttacat tgccacagca tcgtgatctt gatcactaat gaatagaaaa tttcgctatt
    38701 aaaaaaacaa ttaacctgca tttttggtta tccgcaaacg gattacctga tgataacgct
    38761 aagcaatggc atcattgacg ttaaaaagat gttttaaaag aggataaaga aaggttatca
    38821 atggggtaat cggtgttacc ccttttcttc agatgtcaac tttctctttg tattcacaaa
    38881 gatcttcaat aatacaagag ccacagcggg gcttgcgggc aatgcaggta taacgcccgt
    38941 gcaggatcaa ccaatggtgg cagtcgactt taaactctgc cggaaccact ttcagtagct
    39001 tttcttctac ctgttcgacg tttttccccg gcgcaaattg agtacgatta caaacgcgga
    39061 aaatgtgcgt gtcgacagca atagtcggcc agccgaatgc agtgtttaat acgacgttgg
    39121 ctgttttacg acctacgccg ggcagggctt caagcgcagc acgatcttcc ggaacctcgc
    39181 tattatgctg ctccagtaag atacggcagg ttttgatgat attttctgct ttgctgttat
    39241 aaagcccaat cgttttgata taggttttca ccccttcaac gcccagttca agcatcgctg
    39301 caggcgtatt cgccaccggg tagagtttcg ccgtcgcctt attaacactg acatcggttg
    39361 cctgagcgga aagcagtacg gcaatcagca attcaaaagg cgaactgaaa ttaagctcag
    39421 tggtgggatg aggattgttc tcacgcaggc gagtgaggat ctccaggcgt tttgctttgt
    39481 tcatcagaca ttccctgttt caccgtttgg caatgcacgt tcggcagctg cttcagtacg
    39541 gcgctttttc attctttcat caatcaggta ttttcctgcc agcatcagtc ccaggccaat
    39601 aaatgcacct ggtggcagca tcgccagcag gaaaggggag tcggtgtgga aaatctccac
    39661 gcgtaatact tttgcccagc tacctaacag cgcatctgca ccgtcaaaca atgtgccatt
    39721 gccgataatt tcgcgtagtg aacccagcac gaacatggcg caggttgcgc ccataccaat
    39781 tgaaaagccg tccagtgccg aaagcgccgg accttttttg gcagcgaagg cttcagcgcg
    39841 gcccacaacg atacagttag tgacaatcag cggaataaaa atccctaatg attgatacag
    39901 gccaaaggcg taggcgttga tcagcatctg tacagcgctg accaccgagg cgatgatcat
    39961 cacgtaaatg ggaatgcgga tctcggctgg cgtccagtga cgcagcgtcg aaatggtcag
    40021 gttggtcagc gtcagtacca gcgtagtcgc aagtcctaaa cccagagcgt tagtggcagt
    40081 agacgtgaca gccaacagag gacaaaggcc gagcaactgg accagcgcag agttgttttt
    40141 ccacaacccc tgaacaataa cgtctttaat ttcgctcacg ctttattctc cacaggcagg
    40201 aagttgagaa agttgttccg gtaacgtctg agcgtacaat ccggcgcgtt ttaccgcatt
    40261 aaccaccgcg cggggagtaa tcgtcgcgcc ggtgaactgg tcgaaattac caccatcttt
    40321 cttcaccgcc cagtgcgcat catccgcacc actgattttt ttacccgcaa aatgggtgat
    40381 ccagtcagaa aggcgcagtt cgattttatc gccaagccct ggcgtttcgt ggtgctctgt
    40441 cacgcgcgtg ccaagtaccg tgccgttaaa atcggctccc accagcagct gaatcgcacc
    40501 ggaatagcca tctggcgcgg ttgcttccag aacggcggct accggtttgt catcctgttt
    40561 ggcgatgtaa acccgatgct cacctttacc taactctggc gcagttacca gatagcaact
    40621 ctgtgccagc gcattgttat agcgttcggc tggcagcacc tgatcaaata aagccttttg
    40681 ttgcagactg gcctgttcag caatcgtcgt tttggtcatc tggttgatgg ccgcagttaa
    40741 ccctgttgaa cccgctgcaa atagcgccaa cgtaatgccg tgttttcgga tagttttcag
    40801 catggtttac cctttgcgat ggccgtagac gcgcggacgc gtgtagtaat cgatcagagg
    40861 aaccgtgatg ttcgccagca ggacggcaaa agccacgccg tcaggatagc cgccgaaact
    40921 gcggatcaac cagactaata agcccgcaag cgcgccgaaa ataagacgac cacgattggt
    40981 cgtagaagcg gtaaccgggt cagtcaaaat aaagaatgcg ccgagcatgg tcgctccaga
    41041 cagcagatga atttgcggtg ctgccagtgt ttctggtgag aacaaccagc ccaacgttgc
    41101 gcataacgcc agcgttacta agaagctgag gggaatatgc cagcgaatcg ctttctgcca
    41161 tagcaaccat acgccgccag ccagccaggc gagatttacc cattgccaac cagcgcccgc
    41221 cagaataccg ctgtagatcg gatattgcat aatctgttca accgaatgac cggcacggac
    41281 agaggtttta aatgtatcca gcggtgtcgc ctgactaatg ccatcaatac ctaagcgtag
    41341 tgtgttcata tcaccaccac tggcggtatg cccgctgaaa ataacctgta tggcgtcgat
    41401 aaaaccaggg atgttgaccg caatttcatg tggcggtaac cagctggtca tctgcaccgg
    41461 gaaggagatc agtaagacca cataaccaat cattgccgga ttaaacgggt tttgccccag
    41521 accgccatac aactgtttag cgatgataac cgcaaacacc gtacccagca cgaccatcca
    41581 ccatggcgcg agggggggaa tacttaccgc cagcaataag cctgtcagca atgctgagtt
    41641 atctttcaac gtggcggcta ccgactgctt gcgtagtttg agtacgagag cttcggctaa
    41701 cagagcacta accgatgcca acaggatctg aacgagagta ccccaaccaa aaaaccacag
    41761 ttgcgctgcg attcctggca cggctgcgag caacaccaac agcataatgc gcgatgtctg
    41821 gcgctggtta tgggtataag gggagctagc tattctgaat accatttagt cctcgtttac
    41881 aaccttttgc tggacggctt ttttggcctg aacgcgggca atagccgcgg caactgccgc
    41941 tttgcgcgga tcaacctgtt cttctggttc tgcattagcc tgttgctgtt ccagcttgcg
    42001 tgctttggcg cgtgcaatag ccgctgcgac ggcagctttg cgcggatcaa cctgttcttc
    42061 tggctccgca ttagcctgtt gctgttccag tttgcgcgct ttggcacggg taatagcggc
    42121 ttcgacggcg gctttgcgcg gatcgaccgg ttcttctggt tccgcattag cctgttgctg
    42181 ttccagcttg cgtgctttgg cacgggcaat agcggcttcg acggcggctt tgcgcggatc
    42241 gaccggttct tctggttccg cattagcctg ttgctgttcc agcttgcgcg ctttggcgcg
    42301 ggcgatagcg gcttcgacgg cggctttgcg cggatcgacc tgttgttctg gttccgcatt
    42361 agcctgttgc tgttccagct tgcgtgcttt ggcacgggca atagcggctt cgacggcagc
    42421 tttgcgcgga tcgacctgtt gttctggttc cgcatttgcc tgttgctgtt ccagcttgcg
    42481 cgctttggcg cgggcgatag ctgcttcaac ggcagtttta cgtggatcag taacggttgc
    42541 tgcgtcgtta gtttgctgca gttctgcctg tttcgctctg gcttgcgctt tacgggcttc
    42601 ccgtgctgca ataattgcac tgttatccgg gcgttcgccc gctttaatca caataggctg
    42661 tgtagcctgg gcctgtttct ctttcacccg cgccagagcg gcagcaatcg catctttatc
    42721 tttggctgca ggttgaacgg ctgcgctctt atgtcgttca aggcgagccg ctttttcgcg
    42781 ctccagacga gcctggcgcg cttcgaaacg cgctttggct tctgcggcgc gcttttcttc
    42841 ctgacgaata gccgcaattt cagctttttc ctgacggaaa tattgcacca ggggaatatt
    42901 gctcgggcaa acccacgcgc aagccccaca ttcaatgcag tcggcaatgt tatgcgtggt
    42961 agctttatcg tgttgctgac ctttgctgaa ccagtacaac tgttgcggca aaagatccgc
    43021 agggcaggcg tcagcacagg cgctacaccg gatgcagctt tgttcttcct gtggttcgcc
    43081 aagctcattg gcagagggag ccaacagaca gttggtaatc tttacgaccg ggacatccag
    43141 ccatggcaag gtaaagccca ttagcgggcc gcccataatc accatttgat cggcagaggg
    43201 gcagaatccg gcgtcattca ataaatgacg cactggcgtc cccagccgtg cccagacgtt
    43261 gcccgggcga gcgattgctt cgccagtcag ggttacgaca cgctcggtaa tcggctcgcc
    43321 atcaataacg gcacgtttca ctgcataagc agtgccgacg ttttgcatta atacgccgat
    43381 atcggatgaa cgcccgccat gtggaacctg cttcccggtc agaatgtagg ttaattgttt
    43441 agcaccgcca gaaggatatt tggttggaat cacccgcata gaaatatcgt gagagtccgc
    43501 cagcaccgcg cgcagcatgg aaatcgcctg cggtttgtta tcttcaatgc cgataagaat
    43561 ttcgcgtggc tgcagaatat gcgcaagaat gcgaataccc tctacgacct gagccgcgca
    43621 atcctgcatc aaacggtcat cggcggtaat gtatggctcg cactcagcag cgttgataat
    43681 caacgtctca atcttatctc cgccgccctg caatttaacg cctgtcggga atcctgcacc
    43741 acccagcccg gcaacaccaa actgatgaat gcgctcgatt aactcttcgc gacggcgaga
    43801 gcgataatcg gcccagccgt ctcgtgggat ccagcagtct tcaccatcgg catcaataat
    43861 cacgcttaat tcagctaaag ctgaaggatg agccgtagag tggggcgcaa tagccgtaac
    43921 ggtacccgag gtgggcgcgt gaacaggcag cattttgccg cgaccacggg taagcggctg
    43981 gccgcgcaat actttatcgc cgacgctaac gcacaactca ccttcagcgc caatatgctg
    44041 tttcagtgga atgacaaaac gctgcgccag tggtacctgg cgcaggggtg taccgttgga
    44101 ctgggttttc atctccggtg gatggatgcc gccgttgaaa tcccagattt tattttttct
    44161 gaatgcagag aataacttaa gcatggtgtt ccacgggaat gatacgcacg ggaatggtgt
    44221 tcagatccca tttccaggag tcaggtgttt ctgcgaccgg ttgcaacgag atgcagtgcg
    44281 tcgggcacgg atcaacacat aaattgcagc ccgtacagag atcactcatt accgtatgca
    44341 tggcacgggt agcgccaacg atggcgtcta ccggacacgc ctgaatacat ttagtgcagc
    44401 caatacagtt attttcatca ataaccgcca ccatccgcgc aggcgttagc tcttgcgctt
    44461 cgccatccag cggctgcggc tcgacattaa gcaactcggc aatttttagc atcacagctt
    44521 cgccaccagg ggcgcaacgg ttgatttttt caccgttaca gctgatggct tccgcgtagg
    44581 ggcgacagcc gggataaccg cactgaccac actggctctg cggtaagatt tcgtcaattt
    44641 tctcaacgac cggatcgtct tccaccgcaa aacggcggga ggcataaccc agaatggcgc
    44701 caaacgccag gcccagcagg ctcacggcgg caacggcaat ccagatagca ttcattacaa
    44761 cttcaccaaa ccactaaagc ccataaaggc cagagacata agacctgcgg taattaacgc
    44821 aatggcatta ccgcgaaaag gtgccgggac atcagccaca gcaaggcgtt cgcggatggc
    44881 tgcgaagaga accatcacca gcgaaaaacc gacagcggcg gaaaaaccgt acagcgccga
    44941 ctgcaagaaa ttgtgcccga gattgatatt cagcaacgcc acgccgagca cggcacagtt
    45001 ggtggtgata agcggcaaaa aaatccccag caaacggtaa agcaccgggc tggttttgcg
    45061 caccaccatc tcggtgaact gcacgaccac agcaatcacc agaataaatg ccagggtgcg
    45121 caggtaaatc agattaagtg ggatcaaaat ccacgtatcg ataagccagg cgcaaataga
    45181 cgccagcgtc atcacaaacg ttgttgccag ccccatgccc atcgcggttt ccagcttttt
    45241 ggaaaccccc ataaacggac agagaccgag aaacttgacc agtacaaagt tattgaccag
    45301 tacagttccg acaaacagta acaggtagtc agtcattttt cggcctgaaa taaaaaagcc
    45361 gcctattatc cgttaatcca gagcaggcga caacaggtta actgtaaggt tattacggat
    45421 tcacaaacgt cgctttcacc cgcgacgagc gtttgaaata ggggacgatc aatgcggttg
    45481 ccaacaaagt aaacagcaac tggcgaacgg ctatgccatc ttccacgggt gaaaaagcga
    45541 acgcttttac cgccagcaat acggaaataa gcagccagat aatatagtgt ttaggaacac
    45601 aacgccgacg tttaaagaaa gcgatggtca accacagtgt gtaataccac atggcgatag
    45661 cggtaataaa agaaacgccc cataagattt gtgtcgtcag tgcttgtgcg ccgagcgttt
    45721 gaaatgtttg cggagaagat aacgcagcgg tatacagcaa caacgccagc gtcgtactta
    45781 acagagcgac taaaagccag gccaacgggc caagcaacca gcctccaata cgttgtggcg
    45841 ttgtggtggt cataggttct ccagaatagc gacgcaaaca ggcaaatcta attttagggg
    45901 cgttattata aggcttttat aaccgatcgc tactttcttt attgaacata gtgccagacg
    45961 gacttcggta cctggccgag gtcaaacaaa cgcccgccag aaaccagctc tgcgcgacgg
    46021 tgatcggcag cacgatacat attgatcagt tcctgatcgt ccgtcagggt gtaattaaga
    46081 tggtcgtaga gtttttccag actttcgagt gaactgattt tgcgaaattt taataagtag
    46141 tcctgaacag tcataaaagg tccataaaat aattaatacc aaaggggtag gtattattag
    46201 tgatgataaa atttcaccaa aagtgaacgc ttgtgttatt acaataataa gaatgcgctg
    46261 atggcaaata ttcagggatg aaaaactgct atgaaaagag taacgcgctt tactatccct
    46321 gacaatcacc aacaacaccg aacaagataa taaattcctg gtttaatatc cgacaagtga
    46381 aaacatgcac cctgacgggc agcatgtcgc tctacaagcg cagggcttac ttgtgttgta
    46441 ccgtagtact ctgttcaggt ggctgatagt tgtcaatgtg actcgccacg ccaagaagaa
    46501 tgactgaaac gacaagaacg atccaacctg ttaattcaat aagacgattc attacaactc
    46561 acatctctgt tgattgatcc attaacttca gggggtaaat gttacttagc aataatagct
    46621 ccagcaagat ttttactgag gtttttgcga tatttagctt ttgtcgttgg aaaaatcgct
    46681 atttttttga cttaagttaa acaacatccc ttattgctgg caggttatta aactgttgag
    46741 cgagggtaaa ggatagtgtc aaatagccat catacttcaa tgagaggcaa tgacatgagc
    46801 gacaacatcc gtgttgggtt gattgggtat ggttatgcga gcaaaacctt ccatgcgccc
    46861 ctgattgcgg gcacacccgg gctggaactg gcggtaatct ccagcagtga tgaaacaaaa
    46921 gtaaaagccg actggccaac ggttacggtt gtctctgagc cgaagcatct gtttaacgat
    46981 cccaacatag acctgattgt cattcctaca cccaacgata cccatttccc gttagccaaa
    47041 gcagcgcttg aggcgggtaa acatgtggtc gttgataaac cctttaccgt gacactgtca
    47101 caagcgcgag agctggatgc gctggcaaaa agcctggggc gtgttctgtc tgtattccat
    47161 aaccgtcgtt gggatagcga tttcttgacg ctaaaaggtt tgctcgcgga aggcgtgctg
    47221 ggtgaagttg cttactttga gtctcatttt gaccgcttcc gtccgcaggt gcgcgatcgt
    47281 tggcgtgaac agggcggccc aggcagcggt atctggtacg atttagcacc acatcttctt
    47341 gatcaggcca ttacgctatt tggtttaccg gtcagcatga cggtagattt ggcacagtta
    47401 cggcccggag cgcagtcgac cgattatttc cacgccatct tgtcctatcc acagcggcga
    47461 gtcattttac acggtaccat gctggcagcc gctgagtcag cacggtatat cgtgcatgga
    47521 tcccgaggca gttatgtgaa atatggcctc gatccacagg aagaacgtct gaaaaatggc
    47581 gagcgtctgc cgcaggaaga ctggggctac gatatgcgtg atggcgtact tacccgcgtg
    47641 gaaggtgagg aacgtgtcga agaaacgctg ttgacggtgc ctgggaatta tccggcttac
    47701 tatgcggcga ttcgtgatgc gttaaatggc gatggtgaaa atccggttcc ggcaagccag
    47761 gcaatccagg taatggagtt gattgagctg ggcatcgaat ccgccaaaca tcgcgcgact
    47821 ttgtgccttg catgaaaaat ggatggtgca atttgcacca tccatttttg ttacttcgct
    47881 gcgacttttt ctcgcagtgc gcgtttttcc tctgcgttga ggaaagccat ttccagacca
    47941 ttaatctgtg cctggcggat ttgctcgcgg gataacccag cagctggcgc ggcaacggta
    48001 tattcgtgaa tgatatccac tccctgtacg ccgggatcgt cagtgttaat gctggcacga
    48061 atgccatgct caaggaacgt tttcagcgga tgtgcagcca gctctgccac ggtgctggtc
    48121 tgaatattgg aggtcagaca ggattcaata ccaatttgtt gctcggcgag aaaatccatc
    48181 agcgcccgat cttcaatggc ttttacgcca tgtccaatgc gctctgcccc cagttcacga
    48241 atcgcctgcc agatgctttc cggcccggca gcttcgcctg catggacggt aatatgccag
    48301 cccgcatcac gcgcgcggtt gaagtgagaa agaaacagac ttcccgggaa accaagttca
    48361 tcaccggcta aatcaagtgc ggtaatctgg tcacggtggg ctaaaaaggc ctccagctct
    48421 tgctgacagg cggcttcgcc gaaggttcgg ctcataatgc cgataagctt cgcctgcaca
    48481 ccaaaggtgc ggcaaccttc acgtacgcca tcgatcaccg cttcaacaac acctgcaaca
    48541 ggcagctgat gtgccattgc catgtagcct ggtgaaaaac gcagctcgac atagtgcagg
    48601 ccgttacggg ctgcatcttc aatgttttca aatgccacgc ggcgacaagc atcaagagag
    48661 gcgagaactt taacgcccca gtcaagtttg gtcagaaagc tcaccagatc gggttcgttg
    48721 gcaatgacct gaacgtgggg aatcagtgtt tccagggatt gtgcaggaag cgagatatta
    48781 tactggcggc caagttcaag aatggtctgg ggacgaatgt tgccatcaag gtggcgatgg
    48841 atatcagtta atggcagggt ggtatcaatc atggtcgcac tctttttctg gatataaaag
    48901 tgcgcaccat tataaaaaca aagcagggta aaaatccacg attgcgcaac gattttttac
    48961 gttgcgcaat tggggttagc gaacagcgcg gatggcgtta attaatccag ccacaccttt
    49021 ttccagtttc gaacgtgggc agccggcatt gagtcggaca aaaccacgac cttcttcacc
    49081 gtaggtatac cccggcatga tcgcgacttt ttcttgttcg ataagtgctt tttgcaacgc
    49141 gttgtcgtca atattcaacg gacgtaaatc aagccaggcc agataagtgg attgcgggat
    49201 ctgccagttg agttcaggaa acgcggcgtt cattttatct gcgatatacg tcaggttatc
    49261 tttcagatag acgcgtaagg catccagcca cggtgcacct tgctgatagg cggcgatatg
    49321 ggcagttaac gccagtaccg aaggggaaga aagcccatca cgtcctttca gtgccgataa
    49381 ataggcatca cggctgctgc tattttctat aatcccgtaa gcaccggtca gcgcgggaat
    49441 attgaaactt ttcgatcccg acgtcagcaa cgcccagtct ccccgcgcca cattactcca
    49501 gggaatatgc ggctgctcgc cccaaaccat atccatatgg atttcatcgg aaataacccg
    49561 cacaccatga cgctcgcaca ggtcagccat gatctccagc tcatcgcacg tccacacttt
    49621 tccggtagga ttctgtgggc tacacaggag cataatttta cattctggtt tcgccaacac
    49681 ggcttccagc ttgcccatat cgcaaaacca gccgtcagcc tgcttctcta aagcaacggg
    49741 cattactgtg cgctgattac cttcaatggc cttgtaaaat gcgtcatagg cgggtgtgtg
    49801 gatcaccacg ccttcacctg tttcagacca ctgacgaatc agttctgaga ccatatagat
    49861 aacagaaggg ccatacacca ccgactgagg atcgatggcg gtgtaatgct gggtggaaaa
    49921 ccagtgggca atagccgcga gaaactcatc gttcttccag cggctgtagc caaacacgcc
    49981 gtgcatcagg cgctgattca gcgcctcgat aatgcagggg gcagtggcaa aatccatgtc
    50041 tgaaatcgtg aaaggtaaca ggtcagcagt gccgaaacgg tcagcgacat aatcccactg
    50101 cgtacaccat gtgccatgac gatccacgac ctttgaaaaa tcgaacatat cttatcctta
    50161 tgcctggaca gtatgcatca gaccggccat ttcatctttt actgactgaa cttgtggccc
    50221 gataacaacc tgcaggttat gttgattaag ttgtactacg ccaattgccc gattgtcctt
    50281 cagtgcctgc acattaacaa gcgacatatc tttcacagac aaacgcagac gggtaatgca
    50341 gttatcgagg ctaacaatat tgtcggcacc gcctaaggct tcgaggattg ccggaacgtt
    50401 gtaacctgat ttacccggcg caccggcaac ggctttttcg attgaactgg ccacttcgct
    50461 atcgcgcccc ggggttttca gattgaagcg ggtgatagcg aaacggaaga tgacgtagta
    50521 aacgacaaac cagattgcca ccaccactgg caccatgtac cacttggttg acagaccatg
    50581 caaaataccg aacaccacga agtcgatgat attgccgtcg gtattaccga tggtgacgcc
    50641 gagcacagac atgacggtga agccgaggcc ggttaacagc gcgtggatga catacagaac
    50701 tggcgctacg aacaggaaca ggaattccag cggttcggtc gtgccgccaa cgacacaggc
    50761 gatcaggcca gaaatcagca gccctttaat tttatggcga ttttccgggc gcgcgcagtg
    50821 atacatcgct aacgctgcac ctggcagacc gccgagaaac gcaggcattt tgccttgcga
    50881 aaggaaacgc gtggcgcttt cagaaaaacc gtgagtggtc gggcaactca attgcgcctg
    50941 gaagatggtc aatgcgccgc tgacggtttg accgcagact tcctgcgtgc cacctgcgtc
    51001 ggtaaagcga attaatgcca ccagaatgtg atgcagacca aacggcaaca gcagacgttc
    51061 accggtacca aacagcatcg gtccgaaatc acccgcgcta ttgatcatat ggcctaagcc
    51121 gctaataccc atggcgaaaa tcggccagac taatggaatc accaggccga caaggcccat
    51181 caccagcgag gagatgattg gtacgaagcg cgtaccgccg aagaatgcca gcgcatccgg
    51241 caggcggata ttatggaatc gctcatgcag catccagacg ataataccgg cgatcaccgc
    51301 accgaggatc ccggtgtcga tcgactggat cccaagaatg ctctggatgt tattggcttt
    51361 cagaacggcg gcatccgtgg ttggcagaat gcctttattg gtcaaccaga agtttaccgc
    51421 gaggttcatt accgcataac cgacgaagcc agcgaatgcc gctacgcctt tgttttctcg
    51481 tgccagaccc agcgggatgg cgatacagaa catgactggc aggaaactaa aagcaaacga
    51541 gccaatctta ctcatccagg taaagatagc ctgcaacacg gggttgccca ggaccgggag
    51601 cagggtgatg acatcgtggc tactaaggga actaccaatg ccgagcataa tgccgcagaa
    51661 cgacaataat gccacgggta acatgaaggt tttgcctaac tgctggaaga actcccacag
    51721 cgtgactttc ggtgctgttt tcgccgtcat aaaacgactc ctcgtagaga acaatctgga
    51781 ttcagtaaat tgcgcgagaa gataaaacgt tttatcaaat tttagtgagg cataaatcac
    51841 attacgcaac gataatagcg ggtataagat aaataaaagg taaaacgttt tatctgtcac
    51901 aaaatcaggg agtaggtcat ctgcatggct accgccaaaa aaataaccat tcatgatgtt
    51961 gcgctggctg cgggcgtgtc ggtaagtacc gtttcgctgg tgcttagtgg caaagggcga
    52021 atctctaccg ccacaggaga acgcgttaac gccgccattg aagagctggg atttgtgcgc
    52081 aatcgccagg cgtcggcgct gcgcggtggg caaagcggcg tcattggttt gatcgtccgt
    52141 gatttatctg cgccgtttta cgccgaattg acggccggat tgacggaagc tctggaagcg
    52201 cagggacgga tggttttttt gcttcacggc ggtaaagacg gcgagcagct ggcacagcgg
    52261 ttttcactgt tactgaatca gggtgtcgat ggtgtggtaa ttgccggggc tgcaggaagc
    52321 agcgatgacc tgcgacggat ggcagaagaa aaagctatcc cggtgatttt cgcttcccgt
    52381 gccagttatc ttgatgatgt tgatacggtt cgcccggaca acatgcaggc tgcacagttg
    52441 ttgacggagc atctcattcg caatgggcat cagcggatcg cctggctggg agggcaaagt
    52501 tcctcattaa cccgtgcaga acgggtgggg ggctattgtg caactctact aaaatttggc
    52561 ctgccgtttc acagcgattg ggtgttggag tgcacttcca gccagaagca agccgcggaa
    52621 gctatcacgg cgcttttacg tcataacccg accatcagtg ccgtggtttg ctataacgaa
    52681 actattgcga tgggggcatg gtttggtttg ctgaaagcag ggcggcaaag cggggaaagc
    52741 ggagtcgatc gttactttga gcaacaggtt tcgctggcgg catttaccga tgcgacacca
    52801 accacacttg atgatatacc cgttacctgg gccagcacgc ctgcgcggga acttggtacc
    52861 acacttgcgg atcgcatgat gcaaaaaatc acccatgaag agacgcattc acgcaatctt
    52921 attattcccg cccggctcat tgcggcgaaa taatcctctc tttatctgct atacctggta
    52981 gtgtcctttc ctcaagttta atgataaaaa atgtggcaca caggcggtgc cttttatttt
    53041 tactgaagtt cacaggaggt ttatgtgttt aattctgaca acctgagact cgacggaaaa
    53101 tgcgccatca tcacaggtgc gggtgcaggt attggtaaag aaatcgccat tacattcgcg
    53161 acagctggcg catctgtggt ggtcagtgat attaacgccg acgcagctaa ccatgttgta
    53221 gacgaaattc aacaactggg tggtcaggca tttgcctgcc gttgtgatat tacttccgaa
    53281 caggaactct ctgcactggc agactttgct atcagtaagc tgggtaaagt tgatattctg
    53341 gttaacaacg ccggtggcgg tggacctaaa ccgtttgata tgccaatggc ggattttcgc
    53401 cgtgcttatg aactgaatgt gttttctttt ttccatctgt cacaacttgt tgcgccagaa
    53461 atggaaaaaa atggcggtgg cgttattctg accatcactt ctatggcggc agaaaataaa
    53521 aatataaaca tgacttccta tgcatcatct aaagctgcgg ccagtcatct ggtcagaaat
    53581 atggcatttg acctgggtga aaaaaatatt cgggtgaatg gcattgcgcc gggggcaata
    53641 ttaaccgatg ccctgaaatc cgttattaca ccagaaattg aacaaaaaat gttacagcac
    53701 acgccgatca gacgtctggg ccaaccgcaa gatattgcta acgcagcgct gttcctttgc
    53761 tcgcctgcag ccagctgggt aagcggacaa attctcaccg tctccggtgg tggggtacag
    53821 gagctcaatt aatacactaa cgaaccggta aacagccgta tgtgttgttt accgggatag
    53881 cgtgtgcaat tcatcaacgt ctctgctaaa caactggcag ccaaatcacg gccattggtt
    53941 aaccaatagc tggatgaaaa gcatacgaat agagtgtgcc ttcgcactat tcaacagcaa
    54001 tgataggcgc tcacctgaca acgcggtaaa ctagttattc acgctaacta taatggttta
    54061 atgatggata acatgcagac tgaagcacaa ccgacacgga cccggatcct caatgctgcc
    54121 agagagattt tttcagaaaa tggatttcac agtgcctcga tgaaagccat ctgtaaatcc
    54181 tgcgccatta gtcccgggac gctctatcac catttcatct ccaaagaagc cttgattcag
    54241 gccattatct tacaggacca ggagagggcg ctggcccgtt tccgggaacc gattgaaggg
    54301 attcattttg ttgactatat ggtcgagtct attgtctctc tcacccatga agcctttggg
    54361 caacgggcgc tggtggtcga aattatggcg gaagggatgc gtaacccaca ggtcgccgcc
    54421 atgcttaaaa ataagcatat gacgatcact gaatttgttg ctcagcggat gcgtgatgcc
    54481 cagcaaaaag gcgagataag cccagacatc aacacggcaa tgacttcacg tttactgctg
    54541 gatctgacct acggtgtact ggccgatatc gaagcggaag acctggcgcg tgaagcgtcg
    54601 tttgctcagg gattacgcgc gatgattggc ggtatcttaa ccgcatcctg attctctctc
    54661 tctttccgcg ggctggtgac aactgagccc gcgtttcata ctttaatttc cctgtacaaa
    54721 atacaatctc gccggagcgg ggaattcctc cctaaaatat tcaccgtagc catatgtcat
    54781 gagagtttat cgttcccaac atgctcgaac gaacgttcgg ttgcttattt tatggcttct
    54841 gtcaacgccg ttttaaagat taatgcgatc tatatcacgc tgtgggtatt gcagtttttg
    54901 gttttttgat cgcggtgtca gttcttttta tttccatttc tcttccatgg gtttctcaca
    54961 gataactgtg tgcaacacag aattggttaa ctaatcagat taaaggttga ccagtatttt
    55021 tatcttaatg aggagtccct tatgttacgt cctgtagaaa ccccaacccg tgaaatcaaa
    55081 aaactcgacg gcctgtgggc attcagtctg gatcgcgaaa actgtggaat tgagcagcgt
    55141 tggtgggaaa gcgcgttaca agaaagccgg gcaattgctg tgccaggcag ttttaacgat
    55201 cagttcgccg atgcagatat tcgtaattat gtgggcaacg tctggtatca gcgcgaagtc
    55261 tttataccga aaggttgggc aggccagcgt atcgtgctgc gtttcgatgc ggtcactcat
    55321 tacggcaaag tgtgggtcaa taatcaggaa gtgatggagc atcagggcgg ctatacgcca
    55381 tttgaagccg atgtcacgcc gtatgttatt gccgggaaaa gtgtacgtat cacagtttgt
    55441 gtgaacaacg aactgaactg gcagactatc ccgccgggaa tggtgattac cgacgaaaac
    55501 ggcaagaaaa agcagtctta cttccatgat ttctttaact acgccgggat ccatcgcagc
    55561 gtaatgctct acaccacgcc gaacacctgg gtggacgata tcaccgtggt gacgcatgtc
    55621 gcgcaagact gtaaccacgc gtctgttgac tggcaggtgg tggccaatgg tgatgtcagc
    55681 gttgaactgc gtgatgcgga tcaacaggtg gttgcaactg gacaaggggc accagcggga
    55741 ctttgcaagt ggtgaatccg cacctctggc aaccgggtga aggttatctc tatgaactgt
    55801 acgtcacagc caaaagccgg acagagtgtg atatctaccc gctgcgcgtc ggcatccggt
    55861 cagtggcagt gaagggcgaa cagttcctga tcaaccacaa accgttctac tttactggct
    55921 ttggccgtca tgaagatgcg gatttgcgcg gcaaaggatt cgataacgtg ctgatggtgc
    55981 acgatcacgc attaatggac tggattgggg ccaactccta ccgtacctcg cattaccctt
    56041 acgctgaaga gatgctcgac tgggcagatg aacatggcat cgtggtgatt gatgaaactg
    56101 cagctgtcgg ctttaacctc tctttaggca ttggtttcga agcgggcaac aagccgaaag
    56161 aactgtacag cgaagaggca gtcaacgggg aaactcagca ggcgcactta caggcgatta
    56221 aagagctgat agcgcgtgac aaaaaccacc caagcgtggt gatgtggagt attgccaacg
    56281 aaccggatac ccgtccgcaa ggtgcacggg aatatttcgc gccactggcg gaagcaacgc
    56341 gtaaactcga tccgacgcgt ccgatcacct gcgtcaatgt aatgttctgc gacgctcaca
    56401 ccgataccat cagcgatctc tttgatgtgc tgtgcctgaa ccgttattac ggttggtatg
    56461 tccaaagcgg cgatttggaa acggcagaga aggtactgga aaaagaactt ctggcctggc
    56521 aggagaaact gcatcagccg attatcatca ccgaatacgg cgtggatacg ttagccgggc
    56581 tgcactcaat gtacaccgac atgtggagtg aagagtatca gtgtgcatgg ctggatatgt
    56641 atcaccgcgt ctttgatcgc gtcagcgccg tcgtcggtga acaggtatgg aatttcgccg
    56701 attttgcgac ctcgcaaggc atattgcgcg ttggcggtaa caagaagggg atcttcaccc
    56761 gcgaccgcaa accgaagtcg gcggcttttc tgctgcaaaa acgctggact ggcatgaact
    56821 tcggtgaaaa accgcagcag ggaggcaaac aatgaatcaa caactctcct ggcgcaccat
    56881 cgtcggctac agccttggtg acatcgccaa taacttcgcc ttcgcaatgg gggcgctctt
    56941 cctgttgagt tactacaccg acgtcgctgg cgtcggtgcc gctgcggcgg gcaccatgct
    57001 gttactggtg cgggtattcg atgccttcgc cgacgtcttt gccggacgag tggtggacag
    57061 tgtgaatacc cgctggggaa aattccgccc gtttttactc ttcggtactg cgccgttaat
    57121 gatcttcagc gtgctggtat tctgggtgcc gaccgactgg agccatggta gcaaagtggt
    57181 gtatgcatat ttgacctaca tgggcctcgg gctttgctac agcctggtga atattcctta
    57241 tggttcactt gctaccgcga tgacccaaca accacaatcc cgcgcccgtc tgggcgcggc
    57301 tcgtgggatt gccgcttcat tgacctttgt ctgcctggca tttctgatag ggccgagcat
    57361 taagaactcc agcccggaag agatggtgtc ggtataccat ttctggacga ttgtgctggc
    57421 gattgccgga atggtgcttt acttcatctg cttcaaatcg acgcgtgaga atgtggtacg
    57481 tatcgtggcg cagccgtcat tgaagatcag tctgcaaacc ctgaaacgga atcgcccgct
    57541 gtttatgttg tgcatcggtg cgctgtgtgt gctgatttcg accttcgcgg tcagcgcctc
    57601 gtcgttgttc tacgtgcgct atgtgttaaa tgataccggg ctgttcactg tgctggtact
    57661 ggtgcaaaac ctggttggta ctgtggcatc ggcaccgttg gtgccgggga tggtcgcgag
    57721 gatcggtaaa aagaatacct tcctgattgg cgctttgctg ggaacctgcg gttatctgct
    57781 gttcttctgg gtttccgtct ggtcgctgcc ggtggcgttg gttgcgttag ccattgcctc
    57841 aattggtcag ggcgttacca tgaccgtgat gtgggcgctg gaagctgata ccgtagaata
    57901 cggtgaatac ctgaccggcg tgcgaattga agggctcacc tattcactat tctcatttac
    57961 ccgtaaatgc ggtcaggcaa tcggtggttc aattcctgcc tttattttgg gattaagcgg
    58021 atatatcgcc aatcaggtgc aaacgccgga agtaattatg ggcatccgca catcaattgc
    58081 cttagtacct tgcggattta tgctactggc attcgttatt atctggtttt atccgctcac
    58141 ggataaaaaa ttcaaagaaa tcgtggttga aattgataat cgtaaaaaag tgcagcagca
    58201 attaatcagc gatatcacta attaatattc aataaaaata atcagaacat caaaggtgca
    58261 actatgagaa aaatagtggc catggccgtt atttgcctga cggctgcctc tggccttacc
    58321 tctgcttacg cggcgcaact ggctgacgat gaagcgggac tacgcatcag actgaaaaac
    58381 gaattgcgca gggctgataa gcccagtgct ggcgcgggaa gagatattta cgcatgggta
    58441 cagggaggat tgctcgattt caatagtggt tattattcca atattattgg cgttgaaggc
    58501 ggagcgtatt atgtttataa attaggtgct cgtgctgata tgagtacccg gtggtatctt
    58561 gatggtgata aaagttttgg ctttgccctc ggggcagtaa aaataaaacc cagtgaaaat
    58621 agcctgctta aattaggtcg cttcgggacg gattatagtt atggtagctt accttatcgt
    58681 attccgttaa tggctggcag ttcgcaacgt acattaccga cagtttctga aggagcatta
    58741 ggttattggg ctttaacgcc aaatattgat ctgtggggaa tgtggcgttc acgagtattt
    58801 ttatggactg attcaacaac cggtattcgt gatgaagggg tgtataacag ccagacggga
    58861 aaatacgata aacatcgcgc tcgttctttt ttagccgcca gttggcatga tgataccagt
    58921 cgctattctc tgggggcatc ggtacagaaa gatgtttcca atcagataca aagtattctc
    58981 gagaaaagca taccgctcga cccgaattat acgttgaaag gggagttgct cggcttttac
    59041 gcgcaactcg aaggtttaag tcgtaatacc agccagccca atgaaacggc gttggttagt
    59101 ggacaattga cctggaatgc gccgtgggga agcgtatttg gcagtggtgg ttatttgcgc
    59161 catgcaatga atggtgccgt ggtggatacc gacattggct atcccttttc attaagtctt
    59221 gatcgtaacc gtgaaggaat gcagtcctgg caattgggcg tcaactatcg tttaactccc
    59281 aatttacact gacatttgca ccgattgtga ctcgcggcta tgaatccagt aaacgagatg
    59341 tgcggattga aggcgcgggt atcttaggtg gtatgaacta tcgggtcagc gaagggccgt
    59401 tacaagggat gaatttcttt cttgctgccg ataaagggcg ggaaaagcgc gatggcagta
    59461 cgctgggcga tcgcctgaat tactgggatg tgaaaatgag tattcagtat gactttatgc
    59521 tgaagtaaaa aataacgccg gagagaaaaa tctccggcgt tttggctgtg aattactgct
    59581 gcggaatagc gggtacgacc ggaacattta atgccggcat tgcaaacata ccaacgaaat
    59641 cttccagtgg cattttctgc ccgtttaacg ttatctgacc gttagtatat tgcaggctgg
    59701 tggtgatggt attgtcctgc aaggtggtca gacggaacat ctgccccatt gctgatgcac
    59761 cttcaacttg ctgtttcgcc agttttttcg cttgatcttc ctgataacct tccagcttcg
    59821 ctacctgagt catcaactca gttgccatat ccaccggaat ggtcagtttc gcatccagag
    59881 atttaaccga acgatctact tcctgcgcca gcgtttgcgg cgcttcttta gtcgttgccg
    59941 gatctttcag gaacagcgac agattcagcg cactttcacc ctgactgttt ttccagctta
    60001 gcggcgcgat agtaatcacc ggatcgcctt tcaacatcag cggcagggcg ctaaagaagg
    60061 cttccgtcac tttctcctga taaagttcgg gattgttggc aatttctggc tgtgccagca
    60121 gcgcctgagt ttgcgcgtta tattgctggc taaactgatg ccaggcttcg ccatcaattt
    60181 ggccgacttt taaagtcagc ttgccgctgc ccagatcctg attctgtacc ttcaggctgt
    60241 ttagcgagta atccagttgg ctattgatcg ttttaccgtc attgaccaga tccgatttac
    60301 cgctgatctc catgccttcc agcagtgcca gttctttgcc ttccactgaa atggtcattt
    60361 tttccagtga cagtttttgg tttcctacgc gctcaccaaa acttgccagc gtgctggaac
    60421 cgtcggtttt cagattatta aaggtcaact gtactttctg gttgtattcg ttaaccgcgt
    60481 ctatccgacc actttgcgcc tccccggaaa gggagatagc tttgccgtct ctgtccgcat
    60541 ttaactggaa ctcgccgccg ctaaaggcga ctttttcatc cttttgctcg taatttagtg
    60601 gcttgagcga aatatcggaa ctggaatcac cgctgtaacc aatgcgcgag ttaatctcaa
    60661 aaggcgtttc accttttgcc atatcaaaca gtggtttgct tacttcgtta ttaaccagcg
    60721 tggtttgaat tgatgccatc gacgggatca ggttcagttt tttaagctgg gcaaggggga
    60781 agggaccatg atcaaccgat tcgttgaaga tgacgctctg accgctttta atccacggat
    60841 tttcttttcc ggcaatgggt ttcaccaaca gttgcagctg actgctgaat acgccgcgat
    60901 gatagttttg ataactcact tccaggttgg attcaggagc ggtcagtttg agttgcgcgt
    60961 tcgcctgcgc gaccatgtct tcgagatggg tttcaatctt cttgcctgta taccatgcgc
    61021 cgcctgtcca gactacgcct agcgcaacaa tgacgcctac cgctaccagc gatttattca
    61081 taatgattat ccataaaatg aaatcaggcg gactggccgc ctgaaggtgt tataagcctt
    61141 taataagctt agcaagagat gttaattttt tcagtaagct cttacagctt gttgtaaacc
    61201 cgtgctaaac ggccgtggcc tttgacagtc accggtgatt cgttggcggc aataaacgct
    61261 gattcacccg gtttaagctg taactgctga gaacctttcc acaacgttgc atcgccttcg
    61321 acgcagaaca aaatggcggc actctgctgg ctaatggtgg tttctttatc actaaggtca
    61381 tgcagcgaga aggcaaaatc atcaacagga atcgggaagt ccagttctgc accttgtttc
    61441 accggctggg tcaacaactg gttagccggt ttggcttcga acttcacatt ggcaaccagt
    61501 tccggaatat caatgtattt aggcgtcaga cccgcacgca gcacgttatc ggagtttgcc
    61561 atcacttcca gcgccacgcc ttgcaggtaa gcgtgcggtg tttcagcgaa caggaacatc
    61621 gcttcgccag gatttaattt caccacattc agcaacagcg gagagaacag gccgctgtca
    61681 tccgggtaaa attcagaaat taaacgaatc gtttgccacg gttcaccctg ctgactgtcg
    61741 agcgccgatt ttaaaatcgc cagcgcgcgg gatttttctt caccctgcat attcaacagg
    61801 ctggcgaaca gttcgcttaa acgttcggca tcaggctgtt gtaaaaagtg agcaatcgcc
    61861 ggatgtgcac ctgcgaccgg ctggagtagg gagacaatct cggaaaattc acgaaacgcg
    61921 ttcatcgcaa ggaaaggcgt cagcgcaaaa accagctccg gcttgtggtt aggatcttta
    61981 tagttacgct cggcggcatc catcgggata cctgcggcat tttctttggc aaaaccgatt
    62041 tcagacttgt gtttgtttgg atgaacctga atggagagtg gctgtgctgc gcataatact
    62101 ttgaacagga aaggcagttc gccaaagcgt ttggcaacgg cctctccgag cagagtcgat
    62161 ttatcactct caatcacatc acgcagtgaa acgatatctc cggcggcatt ctgcactcgt
    62221 gaactgcttt tcggatgtgc gcccatccac agctcggcca tcggctggct ggacggattt
    62281 tccataccat aaagttcagt caacgccgtt ttgctgcccc aggcatagtt ttgcactgag
    62341 ttaatgagtt tttgcatgat caatccctgt tttaatgtgg aaattaatcc cgctattaaa
    62401 gcaagaatcc tacgggaagt aacctggagc cgcaaaaagt cgtactagtc tcagtttttg
    62461 ttaaaaaagt gcgtaggata ttgttactcg cttttaacag ggcaacggaa cacccgccca
    62521 gagcataacc aaaccaggca gtaagtgaga aaacaatgtc aaacaaaccc tttcattatc
    62581 aggctccttt tccactcaaa aaagatgata ctgagtatta cctgctaacc agcgaacacg
    62641 ttagcttatc tgaatttgaa gggcaggaga ttttgaaagt cgcacccgaa gcgttaactc
    62701 tgttggcgcg ccaggcgttt catgatgcgt cgttcatgct gcgtccggcg caccaacaac
    62761 aggtggccga cattctgcgt gacccggagg ccagcgaaaa tgataaatat gtggcgctgc
    62821 aattcctgcg taactccgac atcgcggcga aaggcgttct gccaacctgt caggataccg
    62881 gcaccgcgat tattgttggt aaaaaagggc agcgtgtatg gaccggtggt ggtgatgaag
    62941 cggcgctggc gcgcggtgtc tataacactt atatcgaaga taatctgcgc tactcgcaaa
    63001 acgcgccgct ggatatgtat aaagaagtga ataccggcac caatctgcca gcgcagatcg
    63061 atctttatgc cgttgatggc gacgaataca aattcctctg tatcgccaaa ggtggtggtt
    63121 cggcaaacaa gacgtatctc tatcaggaaa ccaaagcgtt actgacgccg gggaaactga
    63181 aaaattacct ggttgagaag atgcgcacgc tgggtacggc ggcctgtcct ccgtatcata
    63241 ttgcgttcgt tattggtgga acttctgcag aaacgaacct taaaacggtg aaactggctt
    63301 ccgcgaaata ctatgatgaa ctgccaacgg aagggaatga gcacggtcag gcgttccgcg
    63361 atgtggaatt ggaaaaagaa ttgctgatcg aagcgcaaaa tcttggtctg ggtgcgcagt
    63421 ttggcggtaa atacttcgct cacgacatcc gcgtgattcg cctgccacgt cacggcgcat
    63481 cctgcccggt cggtatgggc gtctcctgtt ctgctgaccg taatatcaaa gcgaagatca
    63541 accgtcaggg gatctggatc gaaaaactgg aacataatcc aggcaaatat atcccggaag
    63601 agctgcgcaa agcgggagaa ggcgaagcgg tgcgcgttga ccttaaccgt ccgatgaaag
    63661 agatcctcgc acagttgtcg cagtatcccg tttctacacg cttatcgctt aacggcacga
    63721 ttatcgtcgg tcgtgatatt gctcacgcca aactgaaaga gcggatggat aacggtgaag
    63781 ggctgccgca gtacatcaaa gatcatccga tttactacgc gggtccggcc aaaacgccgg
    63841 aaggttatgc ctccggttct cttggcccaa cgaccgccgg acggatggat tcttatgtcg
    63901 atcaactgca agcgctgggc ggaagtatga tcatgctggc gaaaggcaac cgcagccagc
    63961 aggtgacgga tgcctgtaaa aaacacggtg gcttctacct tggcagtatc ggtggtccgg
    64021 ccgctgtatt ggcgcaggga agtattaaga gcctggaatg tgttgaatat ccggaactgg
    64081 gaatggaagc catctggaaa attgaagtgg aagatttccc ggcgtttatc cttgtggatg
    64141 ataaaggaaa tgacttcttc cagcagatac aactcacaca atgcacccgc tgtgtgaaat
    64201 aaacagagcc gcccttcggg gcggtttttt tacatggcac gaaagaccaa acatttgtta
    64261 tcaaatagta aataatgagt gagctaaaag ttacttaacg aaagcaaaac agaaagaaaa
    64321 aattaatcag gtgaggagta ggccatgaat acagtacgca gcgaaaaaga ttcgatgggg
    64381 gcgattgatg tcccggcaga taagctgtgg ggcgcacaaa ctcaacgctc gctggagcat
    64441 ttccgcattt cgacggagaa aatgcccacc tcactgattc atgcgctggc gctaaccaag
    64501 cgcgcagcgg caaaagttaa tgaagattta ggcttgttgt ctgaagagaa agcgagcgcc
    64561 attcggcagg cggcggatga agtactggca ggacagcatg acgacgaatt cccgctggct
    64621 atctggcaga ccggctccgg cacgcaaagt aacatgaaca tgaatgaagt gctggctaac
    64681 cgggccagtg aattactcgg cggcgtgcgc gggatggaac gtaaagttca ccctaacgac
    64741 gacgtgaaca aaagccaaag ttccaacgat gtctttccga cggcgatgca cgttgcggcg
    64801 ctgctggcgc tgcgcaagca actcattccg cagcttaaaa ccctgacaca gacgctgagt
    64861 gaaaaatcgc gtgcatttgc cgatatcgta aaaatcggtc gaacccactt gcaggacgcc
    64921 accccgctaa cactggggca ggagatttcc ggctgggtag cgatgctcga gcataatctc
    64981 aaacatatcg aatacagcct gcctcacgta gcggaactgg ctcttggcgg tacagcggtg
    65041 ggtactggac taaataccca tccggagtat gcgcgtcgcg tagcagatga actggcagtc
    65101 attacctgtg caccgtttgt taccgcgccg aacaaatttg aagcgctggc gacctgtgat
    65161 gccctggttc aggcgcacgg cgcgttgaaa gggttggctg cgtcactgat gaaaatcgcc
    65221 aatgatgtcc gctggctggc ctctggcccg cgctgcggaa ttggtgaaat ctcaatcccg
    65281 gaaaatgagc cgggcagctc aatcatgccg gggaaagtga acccaacaca gtgtgaggca
    65341 ttaaccatgc tctgctgtca ggtgatgggg aacgacgtgg cgatcaacat ggggggcgct
    65401 tccggtaact ttgaactgaa cgtcttccgt ccaatggtga tccacaattt cctgcaatcg
    65461 gtgcgcttgc tggcagatgg catggaaagt tttaacaaac actgcgcagt gggtattgaa
    65521 ccgaatcgtg agcgaatcaa tcaattactc aatgaatcgc tgatgctggt gactgcgctt
    65581 aacacccaca ttggttatga caaagccgcg gagatcgcca aaaaagcgca taaagaaggg
    65641 ctgaccttaa aagctgcggc ccttgcgctg gggtatctta gcgaagccga gtttgacagc
    65701 tgggtacggc cagaacagat ggtcggcagt atgaaagccg ggcgttaatc tgcaacatac
    65761 aggtgcagcc gtggaatgat caaacgaagc ggctgcgcct gaggtttata acggtgctgc
    65821 acattgtcgg catcgtaatt taacaactca ccaacgtccg gcacgcccgc accattatcc
    65881 cgattaatca gtgcaatcag cggtgtaggg caggcgtgtt ggacttgttt ttgatctcct
    65941 ttgtaccaga cgcgggcaat cggctgcacc ttcaccggac gtttgatttt taacttcgca
    66001 ttctgtggca gggcagcgat atcctgatac tctcgctcca gttttctttg ccactcctcg
    66061 cgcgtccacg gtgcgacact gcgtggtgat ttcaggcttt tttccagctg tgccaggact
    66121 tcatcacgat gcaaattctt aatgatatgt ttattagccc aaccaaagcg taaagtggcg
    66181 ggatcgtgca gaacggtgag cgtgcggtaa gcattaaggg tgatcagccc cggcaaatga
    66241 cgatgcaccc attcaaaacg tgccgcggtg gggagttctg attcaaccgt gacgatatgc
    66301 tcgaacgtgg tcttgagttt attgatgtgc tgaatatgac tgaccaacgc tgcttgcgaa
    66361 aggttatcga cctggtaaca caacacgcca ggcagacgga cagctgcttt gctgctgcga
    66421 ttttcggact gttgttgaat aaataaatga cggaaatgac gcaacgcctg cgactgcgcg
    66481 tcgttgccga gatgttgttt cacctcaata cgattaagcg gattatgctc atcctctttt
    66541 tttacctccg gcaaagagaa cacgcgggca accaatagct tgtgttgctc aagatgagcg
    66601 gcaaatgcag ccagctcttg ttccatctgg cgaaaagtag tgttgagtcg gtctacgaga
    66661 tcgtaacgcg ccataatatt aaccacttta gttacaacat ccttaattta tagcacagtg
    66721 gtggtgactt gaccaccgtg cgcgtagtgt caagcagagg taaattgcgg gatgttatgc
    66781 cataacggcc agctaaacga gaagcgggca ccacccagtt cgctggtgtc acaattaacc
    66841 gtaccgccca ttgccagtgc tatagagtgg acaattgcca gccccagccc gcagccgccg
    66901 gttgagcgat cccggctggg atcgaggcga acaaaaggtt caaagatatg ttcgcggttt
    66961 tctggggcaa tccctgggcc atcatcctca acaattaatg tcgctctatt ccccgacagt
    67021 agcaggctgg tttcaaccgt tgaatggcag tagcgcaggg cattattgag caaattatcc
    67081 agcacgcgct ccattaagcg catatccaac gccgcataat ggccttgcat gagcgttttt
    67141 atccgtaccg ttttatcggg cgttactgcc tgaatatctg ccagatgcgt tgacagccac
    67201 aacggcaggt ctggttcgct aagatgaagc tcgttttgtg ggcgatcgag tcgggcataa
    67261 gtcagcagct cttcaattaa agcttcaagt tgactgatat cacgattcaa cgcctgggat
    67321 tcggcggcgc tcaggttatc gctcatctcc agtcgataac gcaggcgcac taacggtgtt
    67381 cgcagttcgt gagcgatacc gtcaataagc tgttttttgc tggcaatcaa ggcgttgata
    67441 ttgtccgcca tctggttaaa tgcgatacca agtcgttcaa agctcgaacc ctcatcaaag
    67501 tggatacgtt cattgagatg cccatcgcca aatcgttgcg ccgctgcttc cagttttaac
    67561 atatcctgcc agtgcggacg catccagata aacaccggaa aggcgaggga aatagcaata
    67621 aaagcgatca gggcgatatc cagcaatcgc atctgatgga gataataaag ataaggaaca
    67681 ggaccaactg ccagcacgta gtggctgcgc gggatacgct gcaaaaaagt gtactgatcg
    67741 tccagggcga caatttcgcc gccacgcagc cggtgcatgg aaatatcatc aagatggtat
    67801 ttactcagtg gctcgactcg cagatcgaaa gagagattta aatccatctc tttcagcgtc
    67861 ttaccccagt catgtggggg gatctcacgc aattcgctgc gcatcagata cagcgaactg
    67921 ttcatcaaat catccagcga ctgtttgccc gcgcgttcgg cggtaaattt gtacaccaac
    67981 ccaaccagca gagacatcac aaggaagcag acaaacaata acaggtaaaa ctggataaac
    68041 agttttttca tcgcttattc ccatgcatga ggcgcaaaaa gatagccttt gttacgcaca
    68101 gttttaatgc gataaggttc agcggcgtta tcgagcagtt tttttcttaa ccgcgaaata
    68161 gccacgtcca cgctacgatc caggccgtca taactgacgc cgcgtaaatt tttcagcaat
    68221 gcatcgcggt ccatgatttg cccggcatgg gtagctaatt cccacaataa ttcgaaatca
    68281 gctgtcgaga gcgagatttc agtgttagcc agggtgacta cgcggttgat aggatcgatg
    68341 gtcaacgtgc cgaaatgcag ggctttgtag ggagtcagtg acgtttcctg aagacctttg
    68401 gtcagcgtgg cttgctcatt ctgacgcaaa tgcaaacgta aacgcgctaa caaaacagca
    68461 gggggcgtcg ttttgagaat atagtcgcag gcacccattt ccagtgccag gatgtggttc
    68521 atatcgctat cgagagaggt tagaagaaca atcggtccgg accactttgc gcgtaaatca
    68581 cgacaaatgg tcatgccgtc cttgcctggt agcatgatgt cgagtaacac caaatccgga
    68641 ttttctcgca aaatggtttc ttcggcctgg tcgccgcgcg gctctacggt aacctgtata
    68701 tcatgttttg ccaggtacgc ggcaatcagt gaaccgactt ccgcatcatc ttccacaaat
    68761 acgatagtgt tcataacatt cacggtagat ataaaaacgt cacaatacac cgcgctgttt
    68821 ttacttacta tcaatcgttt ctaaacaagt tttcatcagg tattctactg atgacttgtt
    68881 atggaagtgt taaggtaaaa agatggggct ggtaatcaaa gccgcgctcg gtgcgctggt
    68941 agtgctgttg attggtgttt tagcaaaaac gaaaaattat tatatcgccg ggctgattcc
    69001 acttttcccg acctttgcgc ttatcgcgca ttatattgtt gccagcgaac gcggcattga
    69061 agccttacgc gcaaccatca tttttagtat gtggtcgatt attccctatt ttgtttacct
    69121 ggtgtcgctg tggtatttca ccggaatgat gcgcttgccc gccgcttttg ttggttctgt
    69181 tgcgtgttgg gggataagcg catgggtgtt gattatatgc tggataaagc tgcattaacg
    69241 cagatgacga ccgccatcaa gtgggaaact gcgtccggtg acaaagcaac tggtaagtaa
    69301 gtaatcgacc aggtcgatca cttctttctc gccaggcgcg gttttcatca atgatttatt
    69361 cagcgcctgt tgtcgatatt cggcatcatc atgttcatta aacaggatca gcgatggcgc
    69421 aatagaattc actttcactt ccggtgccag cttgcgggca aacgagcggg tcatattatc
    69481 cagtgccgct ttgcttgcag catacgcaat atgtttgtcg ctaccgcgct ccaccacata
    69541 atcggtaaag tgaatgatat cgctggcggc gtgtccgtgc ccacgcagta atctttccag
    69601 cgcatggttg agcaggtatg gggtattaac gtggatctgc atcatgcaag ccagtacgtc
    69661 ggccagtggc gcacccggtt tttccgccat ccacgcactg gcgttatgca aaatagcacg
    69721 cagaccatgg gtgcttttta gtacttcatc ggcaaacgcc atcacaccgt cgttggtcga
    69781 aaaatcagcc tgaatacact gcgcacctgc attaatcagt ccatcaatgg ctggatagtg
    69841 tgtccgatag ctgacaatca ccggttgctt ttgattaatg aaatgccatg cgagggcgag
    69901 gccgatgcga cgacctccgc cagtaattaa tattggcaag ggctgggttt tacccatcgt
    69961 tatctccttt gctatccaac gatgggatga acgttatccc accagcatcc aggtcgccgg
    70021 aactgaggca attaacagca tgccaatcag caccatttcc tgacggttca gtacattatc
    70081 atgtgtatgc gttttgcgcg catacaggaa aaccagcagc cccggtgcat acagaacaac
    70141 ggataacagc aagtgcattg gaccagaggc gtacaataac cataagccat aaatgcaggc
    70201 accgacacct accgctttat gcagtggacg ggttgcgatt ttcagcaaga acgcgccgac
    70261 caggaaatag ggcaccagaa tcatttctga ggcgatggtc agcaacgtgt tgtaatcgga
    70321 accggtcagc cagatgagta ccagacaaat ttgcacgcag atgtttgtca accacagtga
    70381 ggcggatggc gcagcttgtg cgttctggcg cgcaaaaatg cgtgggaatg ctttatgagt
    70441 ggcagccagg aacggaactt ctgccgccat gatggtccag cttaagtacg cgccgcaaac
    70501 ggaaacgatc aaaccggcag cgatgatgat ttcgccccac ggtcccatca tttccaccat
    70561 cagaccggcc attgacgggt tacgaatttc agccagttca ggacgtgcca ccactcccag
    70621 cgaaagcagc gttaccagca agtaaacgcc cagagcggag agaaccgcca gcagtgtcgc
    70681 tttgccaaca tcacgtttat tacgcgcacg cgcagaaaca accacggcac cttccacacc
    70741 aatgaaaacc cacagggtga tcagcatggt gtttttcacc tgttcccaaa caggtacgcc
    70801 aagtgcaagt ccggtgaagt cgagcttgaa gatatccagt ttgaacatca tcatcgccag
    70861 cacaacaaac agacccagcg gcaacaattt tgccagtgtc gccaccaggt taatgctggc
    70921 agcggtttgc accccgcgca ggatcaaaaa gtgaacaatc cacaataaag ccgatgcacc
    70981 gactatcgat tgccaggtat taccatcgcc aaataggcgc aattccggcg tgtccgtaaa
    71041 aaagcttaac gcggaaaaaa cgatcaccag ataggagacg ttggcgatga ctgcgcacag
    71101 ccagtatccc catgcggaac aaaagccgat tagctcgcca aacccttcgc gggcataggt
    71161 aaagataccg ccgtcaagtt cgggacgaat gcgcgtgagg atcagcatgg caaaggccag
    71221 caataaaatg ccagcgccag taatacccca gccgatgagc agtgctgccg ggctggcaac
    71281 tgccgccata ttttgcggca gactgaaaac acccgcgccc agcattgagc ttaataccag
    71341 cgcggtgagt gcgctcagtc ccagtttctt ttccatcgat atccagtgat taaaacgtga
    71401 taagaagaat aaatattagg gcaaaacgca taaaaatgga gtttgccact gaagcgcggg
    71461 attttacgaa gagatgtgtc tgcatgcaat ggtcgcgggg aaatttgtga ctaaaaatgc
    71521 gaaaggcggc atcaagccgc cttataagag taactgaata gttatttata cagatctgca
    71581 ctgacggtca ggttattacc acgttcctgc cactggcggg tgatgtggta atatttagca
    71641 cctttcttcg cggctcgttt cgcaacctga taggagactt cggtcatgtt gccgtagttg
    71701 ccagagaatt tgatgctgtc gaacgggacc atcatcgctg ctgtcgcttt gttcagttct
    71761 tcgactttag tgccatccgg aagcgtgaca gtgtaacgcc cgccttttga tgactgggtt
    71821 tcaaagaagc gaccgacttc agaacttggt gatgcggtag tcgcaacacc cgggatctca
    71881 actttcttcg cggcttcgcc accggcagcc agagctgcac gtcctgcttc ggaatctgcc
    71941 gggatcacat ccgggctctg gacgatacgt ttcttagcat cttttttata gatgaatgca
    72001 gtaatacgct ggttgccgcc ctggttggca tcgatttgac gaacgatgta gaaagagtag
    72061 gcacctttcg cttttgccgc tttggtgatg gcatcattga cttctggctg gctacgatag
    72121 aagccctgga cggtgaccgt gtcaaacggt tcaatcagaa cagcctgatc tttcggcagt
    72181 tcgacaacac cgttaattac gcgattactt gtttcttctg ctttttcagc atcggcttta
    72241 tagaggtcag cgaccacacg ccagttaccg ctgttcccaa aatcagaagt gtcgacaaca
    72301 taaaaagagg cggcaccttc tttatctgcg cgacgagaaa cggctttcac cgcttcgcca
    72361 atagcattaa aacgaccggt aaccactaca cggtcaaaag gtttaaccgc tgccgcttgc
    72421 tccggtgtca gttctgttgc tgcgttaacg gagaatgccg tagcagaaag cagtgccgac
    72481 gccaggaggg tgttcttaag cttcataaaa ataatccttc gccttgcgca aaccaggtac
    72541 tggtattgtt attaacgaga aacgtggctg attattgcat ttaaacggtg taactgtctg
    72601 cgtcattttt catatcacat tccttaagcc aattttaatc ctgctcaaat gaccgtctat
    72661 gcttaaaaaa cagccgtatc agcatcatta ctactgaagc aactgaattg tataagttaa
    72721 tttaatgtta agtagtgatt cgtgccgggg cgatgtctcg ttttacccga ccgtcgaaga
    72781 caattatcag tctttatccg gcgttctaag gtgtttatcc cactatcacg gctgaatcgt
    72841 taatattttg cgagttcacg ccgaaatact gatttttggc gctagatcac aggcataatt
    72901 ttcagtacgt tatagggcgt ttgttactaa tttattttaa cggagtaaca tttagctcgt
    72961 acatgagcgg cttgtgtggc tcctgacaca ggcaaaccat catcaataaa accgatggaa
    73021 gggaatatca tgcgaattgg cataccaaga gaacggttaa ccaatgaacc ccgtgttgca
    73081 gcaacgccaa aaacagtgga acagctgctg aaactggggt ttaccgtcgc ggtagagagc
    73141 ggcgcgggtc aactggcaag ttttgacgat aaagcgtttg tgcaagcggg cgctgaaatt
    73201 gtagaaggga atagcgtctg gcagtcagag atcattctga aggtcaatgc gccgttagat
    73261 gatgaaattg cgttactgaa tcctgggaca acgctggtga gttttatctg gcctgcgcag
    73321 aatccggaat taatgcaaaa acttgcggaa cgtaacgtga ccgtgatggc gatggactct
    73381 gtgccgcgta tctcacgcgc acaatcgctg gacgcactaa gctcgatggc gaatatcgcc
    73441 ggttatcgcg ccattgttga agcggcacat gaatttgggt gcttctttac cgggcaaatt
    73501 actgcggccg ggaaagtgcc accggcaaaa gtgatggtga ttggtgcggg tgttgcaggt
    73561 ctggccgcca ttggcgcagc aaacagtctc ggcgcgattg tgcgtgcatt cgacacccgc
    73621 ccggaagtga aagaacaagt tcaaagtatg ggcgcggaat tcctcgagct ggattttaaa
    73681 gaggaagcgg gcagcggcga tggctatgcc aaagtgatgt cggacgcgtt catcaaagcg
    73741 gaaatggaac tctttgccgc ccaggcaaaa gaggtcgata tcattgtcac caccgcgctt
    73801 attccaggca aaccagcgcc gaagctaatt acccgtgaaa tggttgactc catgaaggcg
    73861 ggcagtgtga ttgtcgacct ggcagcccaa aacggcggca actgtgaata caccgtgccg
    73921 ggtgaaatct tcactacgga aaatggtgtc aaagtgattg gctataccga tcttccgggc
    73981 cgtctgccga cgcaatcctc acagctttac ggcactaacc tcgttaatct gctgaaactg
    74041 ttgtgcaaag agaaaaacgg taacatcact gttgattttg atgatgtggt gattcgcggc
    74101 gtgactgtga tccgtgcggg cgaaattacc tggccggcac cgccgattca ggtatcagct
    74161 cagccgcagg tggcacaaaa agcggcaccg gaagtgaaaa ctgaggaaaa atgtgcctgt
    74221 tcaccgtggc gtaaatacgc gttgatggcg ctggcaatca tccttttcgg ctggctggca
    74281 agcgttgcgc cgaaagagtt tcttgggcac ttcaccgttt tcgcgctggc ctgcgttgtc
    74341 ggttattacg tggtgtggaa cgtatcgcac gcgctgcata caccgttgat gtcggtcacc
    74401 aacgcgattt cagggattat tgttgtcgga gcactgttgc agattggcca gggcggctgg
    74461 gttagcttcc ttagttttat cgcggtgctt atagccagca ttaatatttt cggtggcttc
    74521 accgtgactc agcgcatgct gaaaatgttc cgcaaaaatt aaggggtaac atatgtctgg
    74581 aggattagtt acagctgcat acattgttgc cgcgatcctg tttatcttca gtctggccgg
    74641 gctttcgaaa catgaaacgt ctcgccaggg taacaacttc ggtatcgccg ggatggcgat
    74701 tgcgctaatc gccaccattt ttggaccgga tacgggtaac gttggctgga tcttgctggc
    74761 gatggtcatt ggtggggcaa ttggtatccg tctggcgaag aaagttgaaa tgaccgaaat
    74821 gccagaactg gtggcgatcc tgcatagctt cgtgggtctg gcggcagtgc tggttggctt
    74881 taacagctat ctgcatcatg acgcgggaat ggcaccgatt ctggtcaata ttcacctgac
    74941 ggaagtgttt ctcggtatct tcatcggggc ggtaacgttc acgggttcgg tggtggcgtt
    75001 cggcaaactg tgtggcaaaa tttcgtctaa accgttgatg ctgccaaacc gtcacaaaat
    75061 gaacctggca gctctggtcg tttccttcct gctgctgatt gtatttgttc gcacggatag
    75121 cgtcggcctg caagtgctgg cattgctaat aatgaccgcc attgcgctgg tatttggctg
    75181 gcatttagtc gcctccatcg gtggtgcaga tatgccggtg gtggtgtcga tgctgaactc
    75241 gtactccggc tgggcggctg cggctgcggg ctttatgctc agcaacgacc tgctgattgt
    75301 gaccggtgcg ctggtcggtt cctcgggggc tatcctttct tacattatgt gtaaggcgat
    75361 gaaccgttcc tttatcagcg ttattgcggg tggtttcggc accgacggct cttctactgg
    75421 cgatgatcag gaagtgggtg agcaccgcga aatcaccgca gaagagacag cggaactgtt
    75481 gaaaaactcc cattcagtga tcattactcc ggggtacggc atggcagtcg cgcaggcgca
    75541 atatcctgtc gctgaaatta ccgagaaact gcgcgctcgt ggtatcaacg tgcgtttcgg
    75601 tatccacccg gttgcggggc gtttgcctgg acatatgaac gtattgctgg ctgaagcaaa
    75661 agtaccgtat gacatcgtgc tggaaatgga cgagatcaac gatgactttg ctgataccga
    75721 taccgtactg gtgattggtg ctaacgatac ggttaacccg gcggcgcagg atgatccgaa
    75781 gagtccgatt gctggtatgc ctgtgctgga agtgtggaaa gcgcagaacg tgattgtctt
    75841 taaacgttcg atgaacactg gctatgctgg tgtgcaaaac ccgctgttct tcaaggaaaa
    75901 cacccacatg ctgtttggtg acgccaaagc cagcgtggat gcaatcctga aagctctgta
    75961 accctgacgg cctctgcgga ggccgtcact ctttattgag atcgcttaac agaacggcga
    76021 tgctttgacc tcccgctgtt tgttcaagcg caattttgac aataattgtt aacggcacgg
    76081 aaagcagcat acccaccggt cctaacaacc atccccaaaa aatcaacgac aaaaatacca
    76141 ccaatgtgga aagtcccagc ccacgcccca tgatgcgtgg ctccagaata ttgccgaaga
    76201 ccagattaat cagcagatat cccgccagca ccaacaacgc ttcgtagaag ccattaaaca
    76261 ccagtacctg agcaataggg gggattgccg cgagaactga accaatatta gggatgtaat
    76321 taagcgcaaa ggccagcaat ccccagacaa aagcgaagcg aacatcgagg gcggcgagca
    76381 tcgcccatgc gaccaggccg gtgatgatgc tgatggctgt tttcagcacc agataatgag
    76441 acacactgtc aatcgcacgc tgaatcgccg ccatcccttc aaccggacgc gccatcattt
    76501 gcttaaattt tccgggcaat tgtggcactt cgagcagcat aaacagcacc gtcagcagca
    76561 ataaaaatat tgatgacatg gcattagata actgcgtcaa taagttggtg agcaacgtca
    76621 tcgccgcgtt cggatcaatg taatgcgcca gttggtcaac tgagacgtca atccctacgc
    76681 gttgcaacaa cggctcaaga gcttgcagcg gcgtcataat agagttgcga tattgcggta
    76741 acgtccgcgt caactcgttg agcgtggaac ccagataagc taacagcaac accatagcca
    76801 tcacgatgat ggtcatcaaa atcgacactg ccagtacacg cggcacacgc cagcggacca
    76861 tgtgttgcac cagcgggtta agaataacag caataaataa tgcgagaata aacggcacga
    76921 tgatctcggc ggcaaaacgg ataccgcaga gaataatgac cagcattccc aacataatga
    76981 cgatttttag gccattgagc gtgatgagcg gctttgccat gcttgtcccc gttttttctt
    77041 cctttgctaa taataatgtc tttttcgctc cgtcatcata acgaaaatca aaccgctcag
    77101 gtaaagaagt gaaaatattt tgagttaatt cttaagctat gatacaaatc aggcgtgttc
    77161 aactaccgag gacaattatc atccgcgatg acgagaagca acactgcgga taattgtaat
    77221 attatggaca atttgttcag gacgttattt tctacattta cccatcttcg tacctcatcc
    77281 actatcttgc tcgtaggtga gcagcactgg cgcaacgcgc tttagtcctt tcttctgcct
    77341 aagcttgccc tatacgcggg gcttgaccac tttactttgg tttcgctgaa ttaagcgaaa
    77401 attaaaataa ttctcttgca ggagaaggac aatgtatatt tattggattt tattaggtct
    77461 ggctattgct acagaaatta ccggtacgct gtctatgaaa tgggcgagcg tcagtgaggg
    77521 aaatggcggc tttattttaa tgctggtgat gatttctctg tcgtatatat ttctctcttt
    77581 cgctgttaaa aaaatagcct taggtgtggc ttatgcgctg tgggaaggta tcggtatttt
    77641 atttattacc ttgtttagcg ttttgttatt cgacgaaagt ttatcgctga tgaaaattgc
    77701 cgggttaacc accctggtcg ccgggatagt gttgatcaaa tcaggtaccc gtaaagcacg
    77761 taaacctgaa ctggaggtga accatggcgc agtttgaatg ggttcacgcc gcctggctgg
    77821 cattggcaat cgtgctggaa atcgttgcta acgtcttttt gaaattttct gacggctttc
    77881 gtcgcaaaat atttggcttg ctctccctgg cggcggtgct ggctgccttt agtgcgcttt
    77941 ctcaggccgt taaagggatc gacttgtctg tcgcttatgc attgtggggc gggtttggta
    78001 ttgccgccac gttagccgca ggttggatct tgtttggtca acggttaaat cgtaaaggct
    78061 ggattggcct ggtcttgctg ttggctggaa tgatcatggt gaaacttgcc tgatgaagac
    78121 gctgcccgcg ctgtcgggca gcgtttgaac attatttttg cgacagttga tccagcttgt
    78181 cgcgaaaacc ggtaacagaa atggcccggt tatcggcgcg ccagcgatct ttcgcggcag
    78241 gagccgaact ttgcacccca attaattgcc agccgtcatc ggtatgcaac atcagaggcg
    78301 aaccgctgtc acccggcaag gtatcgcact gatgtgacat caccgacgtt tgcgcccagc
    78361 cagtcacttc acagttttga tgactgtaca acgtatcgag atgatcttca gggtatcctg
    78421 cctgagtcac tttacgacct gccgctttta atgcggcggt aagcgcggct ttatctccct
    78481 caaataacgg caacggcgta atgccagaag ggggattacg tagcacaatc aatccgaagt
    78541 cccacggcgc ggctgcggga ggtacaatcc aaccatcccc atctgctttt aaccgctttc
    78601 ccagtgtcgg atcaacgcgg ccttctatgt cgtggatgtc atagcgccaa agacctttat
    78661 ttgacacaaa acgcagcgcc actgctttat cggctttacc ctttggtggc gtcaataaac
    78721 agtgtcctgc cgttaatgcc agattgggtg caatcagcgt cgccgtacat aaattgccgc
    78781 tggccgtttc cagttgccca accgcatccc acggtgattg ggtcgtgtca ttcactggca
    78841 cacgatcatc atgaccaaaa aacagggtgc tgacctcatc gtttgccgat ttggcaacgt
    78901 ctggtttatc tgcaaacaca aaagcagacg tcaaactaat tgcacccaac actacagcaa
    78961 tggttgtacg catatcacac tctggtgggt aattatgatt attaaaagca aaccctcata
    79021 aatactatag acgggacggg atgaaagtgg gagtaaaatc agataactac gatcaggaaa
    79081 gataaaaacg ggcggcaatc agtgcgcata aaataaggat gataagaatg aactcaaagc
    79141 gataacggcc caccatacta ccctccgcta aaggcggtgc taaacgttac ccgcttagca
    79201 ccgtaatttt caggcgcgag ggggcgcgcc agcattactg ttgaaaactt acgctgcggg
    79261 ttgtgcagca ggttttgccg gttgctgatg gctgtgtttc ttggcgtgtt tcttcgctgc
    79321 ctgcgctttt tgttctgggg ctttctgttc agctttcgta tttttatgat gctttttagc
    79381 cgcctgcgct ttctgggcag gtgctgcttt atgctgtttt ttatgatgtg tagttttcgc
    79441 cggcgctgct ttggtggtcg tcgcagtcgg agcaggtgtg gtcgcagtct ctgcagcaaa
    79501 ggcggcagaa gacagaccca tagcagcggc aacaaccaga gctaatactt ttttcattgt
    79561 cataccctca atttgttttt tcatttaacc ccactgcggg gccgttgaaa gaactatatc
    79621 cctgggaatg gcagacttcc gtgagtggtt ggtttcagcg tgtaacgata tgtacaaacg
    79681 ctgaataaat tacagcgttg ataataacgt attgtgactt aaggaaaatt tagctaccaa
    79741 taatagtagt cttgatcggg ttaactattt tactacttac aaagcggtag aataactgcg
    79801 catcactaat aaatgacaca cagcaaaatg aatccgttta tttgggtact tataatcctg
    79861 atgacgctag acgcgctgcg ggaattggct ggcgcttcgt ctattttagg atggctatta
    79921 acgttggttt gagcaggcaa taagtccgga cgggtattta ccgcagtccg gacttatttt
    79981 tcaggcgtgc agacgacgat gcaaacgcgt cccgaccagc agagcaatga ccagcatcag
    80041 agcaataaat gcgccgacgc cgttccagcc atagttatgc cagaaaacac cacccagcgt
    80101 cccggcaata ctcgacccca gatagtaact gaacagatac agcgaggagg cctggccttt
    80161 agcgcgtttt gcgcgggggc cgatccagct gctggcaact gagtgggctg cgaagaatcc
    80221 tgctgagaag agtaacattc cggcaaagat cagccacagc gagctgaata aggtcatcag
    80281 taaaccaaac agcataaccc ccgtcgaaaa caacatcact ggaccacgcc catagcgggt
    80341 ggtcatggtt ccggctttgg gtgagctcca tgtaccggtc aaataagcca gcgataataa
    80401 gccaaccacg gcctgactga catgccaggg ggagagcatc aaccgatagc cgatgtaatt
    80461 aaacagcgtg acgaacgacc ccatcagcaa aaagccttct gcgaacaata acggtaatcc
    80521 ccggtcacgc cagtgcagac gaaagttgat aaacaacgac ttaggtcgca gcgaagtcgg
    80581 gcgaaaatgg cgtgattccg ggaggatttt ccagaacatc aacgccgagg ctagcgcgaa
    80641 acaaccgata gccgccagag caattcgcca gttgaaaaag tccgtgaaga caccactaat
    80701 taagcgtccg ctcatgccgc caattgagtt gccgctgata tacaacccca tcgaaaaggc
    80761 cacgaaactg gggtggattt cctcgctaag ataagtcatg ccaacagctg ccacgccact
    80821 taacgaaagc ccaatcaagg cgcgcataat caaaatgccg tgccagctgg tcatcattgt
    80881 cgaaagtaac gtacaaatgg aggccaatag tagcgccgtt accatcactg gtttgcgacc
    80941 aatggcatcg gatagggggc cagtaaacag caaaccaata gccaacatcg ccgtggaaat
    81001 ggacagtgaa atactactgt tcgcgggcgt taagccaaac tcctgcgaaa gcaccggaag
    81061 gataggttgc acacaataga gaagtgcaaa tgttgccagt ccggcagaga acagcgccag
    81121 ggtgacgcgc ataaattgcg gcgtaccgcg tttaataaat tgatttggct gagaaatgct
    81181 ttgcttgtca gtgtcgcttg ccggagcgcc atcaacagtt gtagtacggc tcacttgaaa
    81241 tccttgctaa atatgcctgt ggatcaggct tatacatagg ttaggaaaat cgaattgttc
    81301 tgtctaatat attaataatc tcaaataaga tgttttaaat atgaatattg aacttcgtca
    81361 tctgcgttac tttgttgctg ttgcggaaga gctgcatttc gggcgcgccg ctgcccgcct
    81421 gaatatttcg caaccgccgc taagtcagca gattcaggcg ctggagcaac aaattggtgc
    81481 ccgactgctg gcacgaacca atcgcagtgt attgctgacg gcagcaggaa aacagtttct
    81541 tgcagatagt cggcaaatcc tgtctatggt ggatgacgct gccgctcgcg ctgaaagact
    81601 gcatcagggt gaagcggggg agttgcgcat tggttttact tcgtcggctc cttttattcg
    81661 ggcggtgtcc gatacgttat cgctgtttcg ccgagattat cccgatgtcc atttacaaac
    81721 ccgcgaaatg aacactcgcg agcaaatcgc tccgctcatt gaaggaacgc tggatatggg
    81781 attgctgcgt aacacagcgt taccggagac gcttgaacac gcagtcatcg tccatgaacc
    81841 gcttatggcg atgatcccgc acgatcatcc cctggcaaat aacccgaatg taacgctggc
    81901 tgaactggcg aaagaaccct ttgtcttttt tgatccgcac gtcgggacag ggctgtatga
    81961 cgatattctc gggctgatgc gacgttacca tttgacgccc gtcatcactc aggaggtggg
    82021 cgaggcaatg accatcatcg gtctggtttc cgccggtctg ggtgtttcaa ttttgcctgc
    82081 gtcatttaaa cgtgttcagc tcaacgaaat gcgctgggtg ccgattgctg aagaggatgc
    82141 ggtttctgaa atgtggttgg tctggccgaa acatcatgaa caaagtccag ctgcgcgtaa
    82201 ctttcgtatt catctgctga atgctctcag gtgagggaaa tttcagcgaa aaagcccgaa
    82261 aaatgtgctg ttaatcacat gcctaagtaa aaatttgacg acacgtattg aagtgcttca
    82321 ccatagccta cagattattt cggagcgcga aaatataggg agtatgcggt ggttgctgaa
    82381 aaccagcctg ggcacattga tcaaataaag cagaccaacg cgggcgcggt ttatcgcctg
    82441 attgatcagc ttggtccagt ctcgcgtatc gatctttccc gtctggcgca gctggctcct
    82501 gccagtatca ctaaaattgt ccgtgagatg ctcgaagcac acctggtgca agagctggaa
    82561 atcaaagaag cggggaaccg tggccgtccg gcggtggggc tggtggttga aactgaagcc
    82621 tggcactatc tttctctgcg cattagtcgc ggggagattt tccttgctct gcgcgatctg
    82681 agcagcaaac tggtggtgga agagtcgcag gaactggcgt taaaagatga ctcgccattg
    82741 ctggatcgta tcatttccca tatcgatcag ttttttatcc gccaccagaa aaaacttgag
    82801 cgtctaactt cgattgccat aaccctgccg ggaattattg atacggaaaa tggcattgta
    82861 catcgcatgc cgttctacga ggatgtaaaa gagatgccgc tcggcgaggc tctggagcag
    82921 cataccggcg ttccggttta tattcagcat gatatcagcg catggacgat ggcagaggcc
    82981 ttgtttggtg cctcacgcgg ggcgcgcgat gtgattcagg tggttatcga tcacaacgtg
    83041 ggggcgggcg tcattaccga tggtcatctg ctacacgcag gcagcagtag cctcgtggaa
    83101 ataggtcaca cacaggtaga cccatatggt aaacgctgtt attgcgggaa ccacggctgc
    83161 ctcgaaacca tcgccagtgt ggacagtatt cttgagctgg cacagctgcg tctcaatcaa
    83221 tccatgagct cgatgttaca tgggcaaccg ttaaccgtgg actcattgtg tcaggcggca
    83281 ttgcgcggcg atctgctggc aaaagacatc attaccgggg tgggcgcgca tgtagggcgc
    83341 attcttgcca tcatggtgaa tttatttaac ccacaaaaaa tactgattgg ctcaccgtta
    83401 agtaaagcgg cagatatcct cttcccggtc atctcagata gcatccgtca gcaggccctt
    83461 cctgcgtata gtcagcacat tagcgttgag agtactcagt tttctaacca gggcacgatg
    83521 gcaggggctg cgctggtaaa agacgcgatg tataacggtt ctttgttgat tcgtctgttg
    83581 cagggttaac attttttaac tgttctacca aaatttgcgc tatctcaatt tgggccagga
    83641 aagcataact tagactttca aggttaatta ttttcctggt ttatatttgt gaagcataac
    83701 ggtggagtta gtgatgctga agcgtttctt tattaccggt acagacactt ctgtagggaa
    83761 aacggtggtt tcccgcgcat tgctacaagc gttagcctcc cagggaaaaa cggttgcggg
    83821 atataaaccc gtagcgaagg ggagcaaaga gacacccgaa gggctgcgta ataaagatgc
    83881 cctggtgttg cagagtgttt caaccatcga actgccttat gaagcagtta atcctatcgc
    83941 gttaagcgaa gaagaaagta gcgtggcgca cagttgccca atcaattaca ccctcatttc
    84001 aaacggcctg gcaaacctga ccgaaaaagt cgatcatgtc gtggtagaag ggactggcgg
    84061 ctggcgtagt ctgatgaatg atttgcgtcc actctctgaa tgggtggtgc aggaacaact
    84121 gccggtgttg atggttgtcg gtattcagga aggttgcatt aaccatgcac tgctaacagc
    84181 tcaggcgatt gccaacgacg ggctgccgct cattggctgg gtggctaacc gaatcaaccc
    84241 aggactggcg cattatgcgg aaatcattga tgtgctcggt aaaaaacttc cggcaccgct
    84301 cattggtgaa ctgccttatc tgccgcgcgc cgaacagcgt gaactggggc aatacatccg
    84361 cttagctatg ctgcgcagtg tgctggcggt agatagagtc acggtgtaac gtccgcgaga
    84421 ttaccgacgc aattacgcag gcaatcaata aaccggggag tagctgatac tccccggtca
    84481 tttcacatat catcaacgtc gacataatcg gcgcgtgcgt ggttgccgcc aacagtgtcg
    84541 ccatcccggt caatccgagt aaaagcgtaa tttcttcgcc atcagggaac cataatccca
    84601 gactacgacc atacaacatg ccaatggcaa gaccgataaa tagcgtcggt gtaaagacac
    84661 caccgggtgc gccggaaccg ctactcgcca gcacggcaca cagtttacaa aggaagatcc
    84721 cggcaatgat cattaacagt ggcggggcgg ttaaaaagga ttgtacggtg ctatagccgt
    84781 tgccccacac tgcaggtgtg aacagggaaa gcagacccac gatcaacccg cctagtgcca
    84841 gttgccaggg cggcgcaagt ttgagactca caaatccacg atgacaggcg ttcattaacg
    84901 ttaacaacag tggtccgcac agacctgcca gcacacctgt actgataatc agcgcatagt
    84961 cacgagcctg aaccgtcact gagagttgta cgctgtagag taacgcgtcg ctatgattaa
    85021 tcagattgct aaccagcaat gccacgacgg cggaaataat caccgggccg agagaggcca
    85081 acatcatagt gccaaacagc acttcggcta taaataaact gccagcaagc ggggcacgat
    85141 aggccgcagc cattcccgcc gcggccccac aggcgatcca taatttccac tcctggcgtg
    85201 gcgtaaaacg ttgggcaaaa caggaggcgg caagggcagc taaaagaatc atcgcacctt
    85261 cgcgaccaat tgcactgccg ctggttacta ccagcagaga ggcaagcgat ttaaccaggc
    85321 ttgctgcgta atcaaactga ccatcggttt gcaacgcttc catgtaatcg gtcggcgcat
    85381 gagggcgttg ctgggtaaat ttctgccagc ccatcaacaa caaacccgcc gccagtccgc
    85441 cgagcgccgg agttagcagc cgtcgccagg gggaaaggtt tgtcgctgca ttgaccagac
    85501 tgccggagtc attattgagg aacaaccact ccagtagcag catcgcatga cgaaacccgg
    85561 caacggcaaa ggccgcgaga ataccgacga ctgttgcgat aagcagacgg cggaacatag
    85621 tgcgcagatc cggataggtg tgtagatgat gcatgagcag aaatgggcaa tgaaacacgg
    85681 gcgactattt tgcggcaaaa gaggccagct gcaaaagcag tgcgtgcttt aagagacaaa
    85741 aaacgtgaca ctgtaacgta ttgttgccga atcgcgcgta atcgtcacca tccggcaata
    85801 ttacggtgat cctatcgaaa gagtggttta accgcgacag gaattaacag ttgtgattgc
    85861 cattgcgcca gcgttaagcg agccagttca cccagtgcac ggtagaaagg gtgttctgct
    85921 ttttcgataa aaacgtcaag aaaacgtgtt gaccagggaa aaaggtgcca tgccagcaat
    85981 tcttcgcatt ctgtctggcg accattctcc gccaaccacg cagccatcaa cagcagtgaa
    86041 ccaaaatgat cttccggttc gttttgcttc atttcaaact gaatgccttt ctcgcgcatc
    86101 cactgacgaa gtgccaatgt tgaatcgcca aacagcacag attcgcgatc cagccagacc
    86161 gacccccacg gcggagacgg cagtgcccac gggccgacaa acaaacgctg ccaggcctgg
    86221 gcgtgagtct cttcacactg tgtctgaaaa gccgtcacca gcggcgctaa tgacgcttct
    86281 ggtagaggcc actgagtttc ccagccgtca ctggtgagta ccgcaaccag aggtgcggct
    86341 tcagcactct ctggagcgta ataaaacagc gcgcccaaca cgcgcgccgc gacagaaaaa
    86401 ttatcttgct gtgaaaaatg ggtcattcct aacatcctga aatgtgcggg ttgccccgca
    86461 ccaatttgtt aacctgcaat agccattcct accgtcatgt gcaggccata aaagagcaca
    86521 cgcccgatca tctcaccgcc aagaatgagg attaacccca gaataagtcc ggcaacgtga
    86581 ggttcacggc gacgtatgag cggacaaagc cagcatccta gacctgcgca taacaatacc
    86641 acacgccaga cctgtaagga ggcgtaatct ggtaccagtg cgctggcttg ttgcacggat
    86701 gagtgaatag acgacagaga caaaccttgc agcacaatca ccccggcgca agcgatcagc
    86761 gccagaacgc taatgatggc aaatggtgtg gtattaaatg taacgcgggc tgcccgcaga
    86821 attgcggcag caagtattgg accactcaac aataccgtca cgaagaaagc cagcgtagtg
    86881 taaccgttat gccaggttgg cacagtgtcg atttgataca cgcaggtcat catccagacg
    86941 aaaatgacgc cgagcgccat gctgacaagt aaccagagtt tccccaacgc ttgcggcatt
    87001 ttaccgatga ccgccaccag ccaccatagt ccgccaacgg caaaaaatat ggaaccggca
    87061 gcaatctcgt tactcagacc agaagctccg attcgattca gcgagttaaa cgcccgcagt
    87121 ggcgatccca ggtgcatgac agaggcaata aacccgatgc ccatcaacag ccagaggaaa
    87181 aacatgccac gaacaatgtg ctgacggtct gcgtcagttt tggcggcgaa ccagccaatt
    87241 ccgctaacaa tcagcgcgcc tactacgcat tgacccaata cggtaaagat aaccagtggc
    87301 cattcatgcc atccatttcc cattttacac ttcctccgga ttagccagat aaccagtggt
    87361 atcgccggtc gggcggctgt tggcgttagg tttgataacg atattgggtt ttgtgaaatg
    87421 cgcgcgcggc agtggcgcga cggcagcaag agtgccgtgc ttctggcgca gctcttcaat
    87481 tggaccgaac tcaagtgcac gcagcgggca ggattcgaca catatgggtt gtttcccctc
    87541 ggcgacgcgc gaataacaac catcgcactt cgtcatgtgc cctttttcag cattgtactg
    87601 tggcgcgccg tacggacaag ccatgtggca gtagcggcag ccgatacaaa catcttcatc
    87661 aaccaccaca aagccatctt cacgcttatg cattgcgccg ctggggcaga cttttgtaca
    87721 tgcggggtca tcgcaatggt tacaggagat ggagagataa taagcgaaca cgttctggtg
    87781 ccagacgcca ttatcttcct gccagtcgcc gccagcatat tcataaatac ggcggaaact
    87841 gacttccggg ccaagatctt taaaatcttt gcacgccagt tcgcaggttt tacagccagt
    87901 acagcggctg gaatcgataa aaaatccata ttgtgtggtc atgggctact ccttaaacct
    87961 tttcgatctg gacaagattg ctgtgcgacg ggtttccctt tgccagcggt gaagggcggt
    88021 gagaggtcag aatattgata ctgccgccgt gatcgacccg gtcaccaaac atatccgctt
    88081 taagccacgc accttgcccg atggcggtaa cgccaggcag aatacgcgga gtcacttttg
    88141 cggcaatcag catttctcca ttattgttaa atacccgcac ggtatcgcca tgacggatac
    88201 cgcgagcctg agcatcaatg gggttgatcc acacctcttg tgggcaggcc tgctgtaaca
    88261 catcaatatt gccgtagctg gagtgggtac gcgctttgta gtggaagccc gttaactgca
    88321 gtggataggt tttccgcagg ggatcgtccc agccatcaaa acctggggta tacgcaggaa
    88381 ggggatgaat aatttcatct tttttcaatt cccaggtatc tgcaatcttc gccagtcgtt
    88441 cagaataaat ttcgattttc cccgaaggtg ttttcaacgg gtttgcctgt ggatcttcac
    88501 ggaatgcgcg gaaagcgacg tagtgttctt ccgggcattt tttcttaaag atcccggtcg
    88561 ttttcatctc ctcgtagtcg ggcatctcag ggttacgttc cttcgttttc gcatggagat
    88621 atttgatcca ttcatgctga ctgcgacctt cagtaaaggt ttgataaacg tccggtccta
    88681 agcgtttggc gacttcactc agcatccagt agatgggttt gcgttcaaat tttgctgagg
    88741 ttgcgggttg ggcgaggatc acatagccca tattccctgc agattcatga gagataaggt
    88801 cttcttgctc tgttggcatc aggtcgggca acaggatatc gcaatactta gccgaggccg
    88861 tcatgaagtg gtcaatgcca acaatcatct cgcacttgct gtcatcctga agcacctcat
    88921 tggtgtgatt gatgtcgcca tgttgattga tcaatgtgtt actggcgtag caccataaaa
    88981 acttgatggg gacatccagt ttttcttttc cacgaacacc atcacgggtc gcggtcattt
    89041 ccgtaccatg gtcgatggca tctgtccatg taaagacgga aatctgcgtt ttaacaggat
    89101 tctcgagcat cgggaaccat tctaccccca gatcccagct accttcgcgt acacctgagt
    89161 tgccgccgtt tatgccgacg ttaccggtga gaacggaaag catggcaata gcgcgggatg
    89221 tttgctcgcc gttggaatgt cgttgtggcc cccaaccctg acaaatataa gcaggttttg
    89281 ctgaaccgat ctctcgtgcc aactggataa ttttttctgc cgggatgctg gtgatttttg
    89341 ctgcccattc cggcgtttta gctatgccgt caggcccttc gcccagaata taggctttat
    89401 aatgcgcgtt acgtggtgcg ttggcgggca gcgttttttc atcgtaacca acacaatatt
    89461 tgtcgagaaa tggctgatcg accatgtttt cactaatcag tacccaggca atcgcacagg
    89521 ccagtgcgcc atcggtgcca gggcgaatgg gcagccattc atcttcacgc ccggcagctg
    89581 tgtcgttata acgtggatcg atgacgatca tgcgtgcgtt tgaacgttcg cgggcttgct
    89641 cgacgtagta agtgacacca ccaccgctca tccgcgtttc tgccgggtta tttccgaaca
    89701 taacgaccag tttcgtattg gcgatatcat ccgggctgtt gccatcattg gcaccgaaca
    89761 tataactcat tgcggcactg atctgtgcgg tactgtagct gccatagcga ctgagaaaac
    89821 caccgcaaga gttcatcaga cggtacggga cgtttgagtt ggtgatgttt ccgccatcta
    89881 cgcctgttcc gtacaggaca tgtacagcct cattgccgta atctttcagg atccgccgaa
    89941 gattatcact gatggtatcc agggcttcgt cccaacttat ccgttcaaat ttaccttcac
    90001 cgcgcttgcc gacgcgcttc atgggatatt tcaacctatc aggatgattc atccgtcggc
    90061 ggatagagcg cccgcgtaaa cacgcacgaa cctgatgatt accgtagacg tcgtcacctg
    90121 tcgtatcaga ctccacccag tacacggtgt catctttcac atgcaaacgt aacagacagc
    90181 ggctcccgca gttaacggtg caggaactcc agaccgcttt ctcttctacc ggtgcctctg
    90241 ccgctcggac catttgggaa aatggcagag tgaaagcact gcttgccagc gcaagactgc
    90301 caagtgcgga ggttttcatc agacttctac ggctgatttc agccttcatg agcgcctctg
    90361 tggtatggat tttcatcatt actcacttat tgcttttcaa acaaaatgtc atgccagaat
    90421 ttatggttgt agtgggttat attttttcga tctcgaccag attcgtgtgc tgcgggtttc
    90481 ccttcgccag tggtgaaggg cgcagagtgg ttagcgtatt cacacatccg ccatggtcga
    90541 ttttatcgcc agacatattg gcctcgtgcc aggctccctg gcccatagcg ctaaccccag
    90601 ggagaatgcg tggtgtcact ttggctggta gccgaacttc gccacgatgg ttatacaccc
    90661 gcaccatatc gccgttggca atcccacgtt tctgtgcgtc tatagggttg atccacacct
    90721 cctgacggca ggcagccttc aggacatcaa tgttgccgta ggtcgagtga gtacgggatt
    90781 tgtaatggaa accaaacagt tgcagtggaa aggtactacg ttcaggggag tcccagcctt
    90841 caaaggttga ggcataaact ggcagtgggc ttatcacttc atctttttcc agttcccagg
    90901 tacgggcaat ttccgccagc ttgctggaat aaatttcaat cttaccggaa ggtgttttaa
    90961 gtggatttgc ctcggggtcg tcacgaaatg ctttgtaggc gacaaaatga ccattgggat
    91021 ctttacgctt atagataccc atttttttca gttcgtcgta agacggtaac gccggatctt
    91081 tggcaagcat tttggcgtac agatgttgta accattgttc ctgcgtgcga ccttcagtga
    91141 acttttgata gacgtcaggt ccaagacgtt tcgcgacttc actcaggatc cagtaaatcg
    91201 gtttgcgttc gaatttttcg ctggtgacag gctggaggaa aatgagatat cccatgttac
    91261 cggcgtagtc gttaggaata atatcttcct gctcaacggt catcaggtct ggcagcagaa
    91321 tgtcggcata ttttgccgat gaggtcataa agttttcgat gaccacaatc atttcgcatt
    91381 tcgattcgtc ctgcagaatt tcatgcgttt tgttgatgtc agaatgctga ttaaccaggg
    91441 tatttcccgc gtagttccag atgaacttaa tgggcacatc cagtttatct ttgccccgga
    91501 caccgtcgcg gattgccgtc atttgtgggc catgatcgat agcatctgtc cagctgaagc
    91561 aggaaattga cgttttgacc ggattatcca gcaccggcag gcgctctatg gtaatggtat
    91621 aggtcgattc acgcgcgcca ctatttccgc cgctgatgcc gacattgccc gtcagaatag
    91681 gcaacatagc aatagcgcgt gcggtcagct cgccgtttgc ctggcgttgc ggcccccagc
    91741 cctggcagat ataagcgggt tttgctgtgc caatttcacg cgccagtttg atgatacggt
    91801 ccaccgggat accggtaatt tgcgaagccc actgcggcgt tttcgctgtt ttatcgtcac
    91861 cttcaccaag aatataggct ttatagtgac catttttggg tgcatctgcg ggtaaggttt
    91921 tttcgtcata gccgacgcag tatttatcga gaaaaggttg atcaacgaga ttttcgttaa
    91981 tcaataccca ggcaataccc gcaaccagcg cggcatcggt gcccgggcga ataggaagcc
    92041 attcatcttc acgaccagca gccgtatcgg tatatcgcgg atcgataaca atcatttttg
    92101 cgttcgattt ttcgcgggat ttttcaagaa gataagtgat gccaccgccg ctcatgcggg
    92161 tttctgccgg gttgttacca aacatcacga ccagcttgct gttttcaata tccgtggtgc
    92221 tgttgccatc attactgccg taggtgtagg gcatggcaca ggaaatttgc gcagtgctgt
    92281 aggagccata ctggttgagt gaaccgccgt agcagttcat caggcgtttg accgccgagg
    92341 ctgatggcga agagcgggtt atattgccgc caacgatccc cgaagagtac tgaatatata
    92401 cagcctcatt gccatattgt tcgacggttt tcttcaggct actggcgata gtatccaggg
    92461 cttcatccca gctaatccgt tcgaatttgc cttcaccgcg tttacccacg cgtttcattg
    92521 ggtaattcaa gcgatcggga tgattaatac gccggcggat ggagcgaccg cgcaaacagg
    92581 cgcgtacctg atggttgcca tactcatcgc tgccggtgtt gtcagtttcc acccaggcca
    92641 cttcattatc tttaacatgt agacgaagtg cacagcggct accacagttg acggaacagg
    92701 caccccagac cacttgttcg ctggcctgtt gtactgctgc cgctgcactg cgcagggtga
    92761 acggcaaaga aaaaccgcct gcagccagcg ccagagaacc tatcgcggta gatttaacga
    92821 gtgttctgcg gctgatgccc accattcgtt catttttgga cataactcac tccctgttct
    92881 ttatcgttat ataaaagttt atatattgaa tatttagcgc gctaacaata gagggagtct
    92941 acccatttgg ggttaataat tattaatcca tatcaataga agggtatgag taatatggtg
    93001 ggattatgtt gtatgtccaa atcgccggat gtgtcgtatc cggcgtgtag tcgataatgt
    93061 attactgcgg ttcggcaggc gcgccatcct gggtagactg cgcgggagca gagacgttac
    93121 cgctggtggt gcgggtgtag agaattttat gcgtatcatt agcgcaatgc ccgacgacct
    93181 gggaatcagg ctgatcaact tggtcattgg gtacaatact taacgtgaag ctgctttcgg
    93241 gtacgccatt attgataatg cgctgtgata tatcgctttg tatgcgctca caggatcccg
    93301 gcgcggcgag taccgcgggt gaggcgaggg cgagcagaag cgcggcataa caggttgaga
    93361 gtttcatcat aagctcctaa cgcgaagata acttctttaa gcatagcatt tcacgtgtaa
    93421 agtactgtat ttgctactat gattgagaat catctctact ctctggtgac tgatgtgaaa
    93481 tacaaattac tgccatgctt actcgcgata ttactcacag gatgtgaccg cacagaggta
    93541 acactttcat ttactcctga gatggccagt ttctctaatg aattcgattt tgatccgctg
    93601 cgtggtccgg taaaagattt cactcagaca ttaatggatg agcaaggtga agtgacgaaa
    93661 cgtgtttcgg ggactttgtc ggaagaaggc tgttttgatt cactcgaatt actggatctg
    93721 gaaaataata ccgtggtcgc tctggtactg gacgccaatt attaccgtga tgccgagacg
    93781 ctggagaaga gagtacgttt acagggaaaa tgccagctag cagaattacc ttctgccggg
    93841 gtgagttggg aaaccgatga taatggcttc gtgattaaag ccagcagcaa acaaatgcag
    93901 atggaatatc gctatgatga tcagggttat ccactgggta aaaccacgaa aagtaacgac
    93961 aaaacattat ctgtcagcgc cacgccatca acggacccga tcaaaaaatt agattacaca
    94021 gcggttactt tactgaataa tcaacgagtt ggtaatgtaa aacagagctg tgaatatgac
    94081 agtcacgcca atccggtggg ctgtcagctg atcattgttg atgaaggagt aaaacccgcc
    94141 gtcgaacggg tttacaccat caaaaatacg atcgattatt attaatgcta ttgtgcggtc
    94201 ggcttcagga gagtctgacc cggtgttttg tgctctgcca gatactgatg ctggaatata
    94261 cacatgcgaa tggcattacg atattgacca ttaataaaga actcgtgcat caattcacct
    94321 tcaaccgaaa agccaagctt gcggtaaatg tgaatcgctt tttcattctc tttatcaacg
    94381 atcagataca gcttatagag attgagaacg gtgaagccat agtccattgc taatttggcg
    94441 gcacgggttg ccagaccttt cccctgatac tccggggaga taattatctg aaattctgcg
    94501 cggcgatgta catggttaat ttccaccaac tccaccagac cggctttttc gccgtcacat
    94561 tccaccacaa agcgtcgttc gctctgatcg tgaatatgct tatcatacag atcagagagt
    94621 tcaacaaagg cttcgtaggg ttcctcaaac cagtaacgca tcacactggc gttattgtcg
    94681 agttgatgta catagcgtaa atcttcacgc tccagcgggc gtagcttaac actgtgggcg
    94741 cttggcataa cgtgtcctta cattccttaa atcaataaca ggttaggggg taataacgcg
    94801 gccagttcga cggtccaggc agcgcaaagt atttggctcc cagtaggcat tgatgttggc
    94861 gctttgctcg catttatcgc ggttatcaaa agcggcgtcg gctttatccc actctttttc
    94921 agtgcgttta ttcactttct ggcgcagatt gcccgtgtca ttccattgct ctttttccat
    94981 agcggcgtgc tggcggcttt gtgcactgtc gccagactca atcaccagtt tgttagtttc
    95041 ggcatgaaca gttgtgctca atgccagtgc gcaaggcagc agaaaagcga gcaggtcgat
    95101 tcgtttgctg agagtgattt tcataattca ttccctgtat gaatgattaa agatgattct
    95161 acaccatcca ctgcgaacgc aaaacgtacc aggagggtgt ttatattgat gatattatgt
    95221 cgccctataa ctatacatga tgtcaataag tgacaaagat gattaaaaca acgttactat
    95281 tttttgctac tgcgctgtgt gaaattattg gatgctttct gccctggttg tggttaaaac
    95341 gaaacgccag tatctggctg ttgcttccgg cggggatttc actggcgctg tttgtctggt
    95401 tgttaacgtt gcatcctgcg gcgagtgggc gtgtttacgc ggcttatggt ggcgtttatg
    95461 tctgcacggc gttgatgtgg ctgcgcgttg tggatggcgt gaaactgact ctttatgact
    95521 ggacggggcc gttgattgcg ctttgcggca tgttgatcat tgttgtgggc tgggggcgca
    95581 cgtaggaaca taaatccatt ttatcaataa gatacgagga agtgtcagct gacaaaaggt
    95641 attctatttc atcttttgtc aaccattcac agcgcaaata tacgcctttt tttgtgatca
    95701 ctccggcttt tttcgatctt catacttata tggtagtagc tcagttgcgt agatttcatg
    95761 catcacgaca agcgatgcaa ggaatcgaac atgaagatcg taaaggctga agtttttgtt
    95821 acctgtccgg ggcgtaattt cgtcacatta aaaatcacca ctgaggacgg tattacacca
    95881 taaatttttg gcttgctgat ttctgcacgc atcgataaga cggatgaatt cctcccgcga
    95941 aagaggatcc ggaatggttc ttgattcctt taatggcgag atccccttaa acgggttatc
    96001 tgccaggtaa ccgttatcaa caccaaactg gaacacggcg ttaagatttg tcatgtaatt
    96061 atttacagtt acagccgatc tccctggttg tgtaacaata tagttacttt tggggatctg
    96121 gtatccagtc agtagctctt tacgaacctc cagtaatttt tctttattaa tcgatgaggc
    96181 aagatttttt tcaccgatta tgctcaggat atttttgatg acggcacggt atgtgttgag
    96241 tgatgttttt gcgacttcag tttctttcag tgccagaaat ttttcagcca gttcttttat
    96301 ggttaaatct tgtcgggcct caccaaattt ttccagattg cgtgaggagg gaaactgttt
    96361 tgcatagtcg aaaacaccag tttttattgc gtaacaaaca gaggcgcgta gctcacctgc
    96421 aacgcgcctg ttttttgctg tgtcaggaac cccaggtttt ccctgactct tacgccttta
    96481 taaacaaacc agatacgtaa tttccctcca tggttttcca cgcctgtcgg atatttcatt
    96541 tcaacttctc tcattagtta gtgtggcttt tagtcaagta agatgacgtc ttggtcttgc
    96601 tgatgcctgg cgctcaatcc agcgatcaat ttcttccagg ttgtaaaagc atggactgtt
    96661 atcccatggc ataccgtcat gagcgacatg cttatattcc cttccttcca taaacgattt
    96721 ttcccgggcc ttttttaacg tacctttttt tattcctttc agcgcaatta actgctcttc
    96781 ggatacccat ttgccgggag agacaatcat gattacttcg ctcatcgatt tctttatctc
    96841 ttacatcaga cgagcgccgg ttgcagaata ccagtcacaa ccggcgacag ttgaacatta
    96901 agaatcagcc tgactcggga tcagtttttg ccagatagct gaaacgtatt ttgcctggta
    96961 acgggcgtca tcaagtgcat tatggcgctc accttcgaat ggaatagccg ttctggcatc
    97021 gaagtctatg gctttcccca gctcaacgat tgtgcgtaca tcgcgatcgt tgtagtaacg
    97081 ccacgggcag gggctcccct gccgttcgta tgaacggcgc aaaatcgtgt tgtcgaagtt
    97141 ggctccattt ccccagacat gaacaaaaaa ttcaccggag ttttcgtcga taaattcccg
    97201 caattgtaac agtgcatcat ctaacgggat ttcatcggtc ataatggcag attgcgcttc
    97261 gcgtgattgt ttaagccacc atttcatggt gtcccgatca atgactccgc cagcagtatc
    97321 cagatcgata gtcttactaa attccggtcc catatctccg gtttgcggat cgaaaaatat
    97381 tgcacctatt gagataatcg gggcatcggg attttttccc atggtttcaa gatcgatcat
    97441 cagatgaatc cccgctctgc tggtggatgt gagattatga tgaccgttcg ccttaattaa
    97501 gggatctgac gcctcgccag tttcactatc gctggcatga tgctgattgc cgccagtgtt
    97561 ctccttgtgc tgatgtgcag taccttccat ttcctccgga tcattttcct gaacttcagg
    97621 ctgattctct ccatcgaata tttcctggta tgttgcgtca cccatcaccg caccacaatc
    97681 agggcagttg ccgccaccgc tctgaccgca ggcggtgcag atcttttccg gttcctgttg
    97741 cactactggt tcaggttgtt tcgtttctgg ctcgttttgt tgcgtatttg ggctgttttg
    97801 ttccgctttc tggtcgttct gttccgtttc ttgctggttc tggttcacag aatcgcgggt
    97861 ctggatcccc ttaacccatt tcggatcatt cgggtcgcta atcccttcaa caaattcacc
    97921 acgtgatacg gcaagcagtt cct
//
LOCUS       DAZFAB010000005        90347 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZFAB010000005 DAZFAB010000000
VERSION     DAZFAB010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 90347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 90347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..90347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.005"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            19..603
                     /gene="yraH"
                     /locus_tag="ACHJWA_000415"
     CDS             19..603
                     /gene="yraH"
                     /locus_tag="ACHJWA_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4307085.1"
                     /translation="MNKVTKTAIAGLLALFAGNAAATDGEIVFDGEILKSACEINDSD
                     KKIEVALGHYNAEQFRSVGDRSPKIPFTIPLVNCPVTGWEHDNGNVEASFRLWLETRD
                     NGTVPNFPNLAKVGSFAGTAATGVGIRIDDAESGNLMPLNAMGNDNTVYQIPADSAGI
                     VNVDLIAYYVSTVEASEITPGEADAVVNVTLDYR"
     gene            683..1378
                     /gene="yraI"
                     /locus_tag="ACHJWA_000416"
     CDS             683..1378
                     /gene="yraI"
                     /locus_tag="ACHJWA_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO4307086.1"
                     /translation="MSKRTFAMIFTLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGTSRAPFIITPPLIRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVSDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKADLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1407..3923
                     /gene="yraJ"
                     /locus_tag="ACHJWA_000417"
     CDS             1407..3923
                     /gene="yraJ"
                     /locus_tag="ACHJWA_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO4307087.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYNFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWDYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHN
                     SSVNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            3934..4667
                     /locus_tag="ACHJWA_000418"
                     /pseudo
     CDS             3934..4667
                     /locus_tag="ACHJWA_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            complement(4710..5570)
                     /gene="rsmI"
                     /locus_tag="ACHJWA_000419"
     CDS             complement(4710..5570)
                     /gene="rsmI"
                     /locus_tag="ACHJWA_000419"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFO4307088.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            5635..7671
                     /gene="lpoA"
                     /locus_tag="ACHJWA_000420"
     CDS             5635..7671
                     /gene="lpoA"
                     /locus_tag="ACHJWA_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFO4307089.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFVGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            7629..8024
                     /gene="yraN"
                     /locus_tag="ACHJWA_000421"
     CDS             7629..8024
                     /gene="yraN"
                     /locus_tag="ACHJWA_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFO4307090.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDTFNDHS"
     gene            8044..8634
                     /gene="diaA"
                     /locus_tag="ACHJWA_000422"
     CDS             8044..8634
                     /gene="diaA"
                     /locus_tag="ACHJWA_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFO4307091.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            8644..9219
                     /gene="dolP"
                     /locus_tag="ACHJWA_000423"
     CDS             8644..9219
                     /gene="dolP"
                     /locus_tag="ACHJWA_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFO4307092.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(9333..10373)
                     /gene="yraQ"
                     /locus_tag="ACHJWA_000424"
     CDS             complement(9333..10373)
                     /gene="yraQ"
                     /locus_tag="ACHJWA_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFO4307093.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(10446..11081)
                     /gene="yraR"
                     /locus_tag="ACHJWA_000425"
     CDS             complement(10446..11081)
                     /gene="yraR"
                     /locus_tag="ACHJWA_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFO4307094.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            11209..11727
                     /gene="yhbO"
                     /locus_tag="ACHJWA_000426"
     CDS             11209..11727
                     /gene="yhbO"
                     /locus_tag="ACHJWA_000426"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFO4307095.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(11707..12150)
                     /gene="yhbP"
                     /locus_tag="ACHJWA_000427"
     CDS             complement(11707..12150)
                     /gene="yhbP"
                     /locus_tag="ACHJWA_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFO4307096.1"
                     /translation="METLTAISRWLAKQHVVTWCVQQEGELWCANAFYLFDVQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRPDEIKFTDNTLGFGKKMIWLRGSGTEQA"
     gene            12201..12503
                     /gene="yhbQ"
                     /locus_tag="ACHJWA_000428"
     CDS             12201..12503
                     /gene="yhbQ"
                     /locus_tag="ACHJWA_000428"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFO4307097.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAKLLSSLQTPEIKSD"
     gene            complement(12490..12993)
                     /gene="yhbS"
                     /locus_tag="ACHJWA_000429"
     CDS             complement(12490..12993)
                     /gene="yhbS"
                     /locus_tag="ACHJWA_000429"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO4307098.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(12987..13511)
                     /gene="ubiT"
                     /locus_tag="ACHJWA_000430"
     CDS             complement(12987..13511)
                     /gene="ubiT"
                     /locus_tag="ACHJWA_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFO4307099.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            13720..14715
                     /gene="ubiU"
                     /locus_tag="ACHJWA_000431"
     CDS             13720..14715
                     /gene="ubiU"
                     /locus_tag="ACHJWA_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFO4307100.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            14724..15602
                     /gene="ubiV"
                     /locus_tag="ACHJWA_000432"
     CDS             14724..15602
                     /gene="ubiV"
                     /locus_tag="ACHJWA_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFO4307101.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            15683..16690
                     /gene="yhbW"
                     /locus_tag="ACHJWA_000433"
     CDS             15683..16690
                     /gene="yhbW"
                     /locus_tag="ACHJWA_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFO4307102.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSARDAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVAWFDARDPNPHVRPVPG
                     YGEQIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(16807..18051)
                     /gene="mtr"
                     /locus_tag="ACHJWA_000434"
     CDS             complement(16807..18051)
                     /gene="mtr"
                     /locus_tag="ACHJWA_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFO4307103.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIDIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(18205..20094)
                     /gene="deaD"
                     /locus_tag="ACHJWA_000435"
     CDS             complement(18205..20094)
                     /gene="deaD"
                     /locus_tag="ACHJWA_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFO4307104.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFSGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(20087..20167)
                     /gene="yrbN"
                     /locus_tag="ACHJWA_000436"
     CDS             complement(20087..20167)
                     /gene="yrbN"
                     /locus_tag="ACHJWA_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFO4307105.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(20274..21158)
                     /gene="nlpI"
                     /locus_tag="ACHJWA_000437"
     CDS             complement(20274..21158)
                     /gene="nlpI"
                     /locus_tag="ACHJWA_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFO4307106.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(21267..23402)
                     /gene="pnp"
                     /locus_tag="ACHJWA_000438"
     CDS             complement(21267..23402)
                     /gene="pnp"
                     /locus_tag="ACHJWA_000438"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFO4307107.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(23649..23918)
                     /gene="rpsO"
                     /locus_tag="ACHJWA_000439"
     CDS             complement(23649..23918)
                     /gene="rpsO"
                     /locus_tag="ACHJWA_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFO4307108.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTRLIERLGLRR"
     gene            complement(24067..25011)
                     /gene="truB"
                     /locus_tag="ACHJWA_000440"
     CDS             complement(24067..25011)
                     /gene="truB"
                     /locus_tag="ACHJWA_000440"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFO4307109.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(25011..25412)
                     /gene="rbfA"
                     /locus_tag="ACHJWA_000441"
     CDS             complement(25011..25412)
                     /gene="rbfA"
                     /locus_tag="ACHJWA_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFO4307110.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(25577..28249)
                     /gene="infB"
                     /locus_tag="ACHJWA_000442"
     CDS             complement(25577..28249)
                     /gene="infB"
                     /locus_tag="ACHJWA_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFO4307111.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(28274..29761)
                     /gene="nusA"
                     /locus_tag="ACHJWA_000443"
     CDS             complement(28274..29761)
                     /gene="nusA"
                     /locus_tag="ACHJWA_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFO4307112.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(29789..30241)
                     /gene="rimP"
                     /locus_tag="ACHJWA_000444"
     CDS             complement(29789..30241)
                     /gene="rimP"
                     /locus_tag="ACHJWA_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFO4307113.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(30448..30524)
                     /locus_tag="ACHJWA_000445"
     tRNA            complement(30448..30524)
                     /locus_tag="ACHJWA_000445"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30488..30490),aa:Met,seq:cat)
     gene            30872..32215
                     /gene="argG"
                     /locus_tag="ACHJWA_000446"
     CDS             30872..32215
                     /gene="argG"
                     /locus_tag="ACHJWA_000446"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFO4307114.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSAASGVPQMENLENKGQ"
     gene            complement(32223..33848)
                     /gene="yhbX"
                     /locus_tag="ACHJWA_000447"
     CDS             complement(32223..33848)
                     /gene="yhbX"
                     /locus_tag="ACHJWA_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO4307115.1"
                     /translation="MTVFNKFARSFKSHWLLYLSVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVAKMLGMYSPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKNAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIVHYKGDS
                     LVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(34307..34393)
                     /locus_tag="ACHJWA_000448"
     tRNA            complement(34307..34393)
                     /locus_tag="ACHJWA_000448"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34357..34359),aa:Leu,seq:gag)
     gene            complement(34408..34740)
                     /gene="secG"
                     /locus_tag="ACHJWA_000449"
     CDS             complement(34408..34740)
                     /gene="secG"
                     /locus_tag="ACHJWA_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFO4307116.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(34968..36305)
                     /gene="glmM"
                     /locus_tag="ACHJWA_000450"
     CDS             complement(34968..36305)
                     /gene="glmM"
                     /locus_tag="ACHJWA_000450"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFO4307117.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVETALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(36298..37146)
                     /gene="folP"
                     /locus_tag="ACHJWA_000451"
     CDS             complement(36298..37146)
                     /gene="folP"
                     /locus_tag="ACHJWA_000451"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFO4307118.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(37236..39170)
                     /gene="ftsH"
                     /locus_tag="ACHJWA_000452"
     CDS             complement(37236..39170)
                     /gene="ftsH"
                     /locus_tag="ACHJWA_000452"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFO4307119.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNAGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(39270..39899)
                     /gene="rlmE"
                     /locus_tag="ACHJWA_000453"
     CDS             complement(39270..39899)
                     /gene="rlmE"
                     /locus_tag="ACHJWA_000453"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFO4307120.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            40025..40318
                     /gene="yhbY"
                     /locus_tag="ACHJWA_000454"
     CDS             40025..40318
                     /gene="yhbY"
                     /locus_tag="ACHJWA_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFO4307121.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(40474..40950)
                     /gene="greA"
                     /locus_tag="ACHJWA_000455"
     CDS             complement(40474..40950)
                     /gene="greA"
                     /locus_tag="ACHJWA_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFO4307122.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            41198..42631
                     /gene="dacB"
                     /locus_tag="ACHJWA_000456"
     CDS             41198..42631
                     /gene="dacB"
                     /locus_tag="ACHJWA_000456"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO4307123.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAVAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(42671..43843)
                     /gene="cgtA"
                     /locus_tag="ACHJWA_000457"
     CDS             complement(42671..43843)
                     /gene="cgtA"
                     /locus_tag="ACHJWA_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFO4307124.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKVEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(43859..44824)
                     /gene="yhbE"
                     /locus_tag="ACHJWA_000458"
     CDS             complement(43859..44824)
                     /gene="yhbE"
                     /locus_tag="ACHJWA_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO4307125.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(44951..45208)
                     /gene="rpmA"
                     /locus_tag="ACHJWA_000459"
     CDS             complement(44951..45208)
                     /gene="rpmA"
                     /locus_tag="ACHJWA_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFO4307126.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(45229..45540)
                     /gene="rplU"
                     /locus_tag="ACHJWA_000460"
     CDS             complement(45229..45540)
                     /gene="rplU"
                     /locus_tag="ACHJWA_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFO4307127.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            45799..46770
                     /gene="ispB"
                     /locus_tag="ACHJWA_000461"
     CDS             45799..46770
                     /gene="ispB"
                     /locus_tag="ACHJWA_000461"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFO4307128.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLKPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            46999..47277
                     /gene="sfsB"
                     /locus_tag="ACHJWA_000462"
     CDS             46999..47277
                     /gene="sfsB"
                     /locus_tag="ACHJWA_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFO4307129.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(47325..48584)
                     /gene="murA"
                     /locus_tag="ACHJWA_000463"
     CDS             complement(47325..48584)
                     /gene="murA"
                     /locus_tag="ACHJWA_000463"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFO4307130.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNTQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(48639..48893)
                     /gene="ibaG"
                     /locus_tag="ACHJWA_000464"
     CDS             complement(48639..48893)
                     /gene="ibaG"
                     /locus_tag="ACHJWA_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFO4307131.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(49053..49346)
                     /gene="mlaB"
                     /locus_tag="ACHJWA_000465"
     CDS             complement(49053..49346)
                     /gene="mlaB"
                     /locus_tag="ACHJWA_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFO4307132.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(49346..49981)
                     /gene="mlaC"
                     /locus_tag="ACHJWA_000466"
     CDS             complement(49346..49981)
                     /gene="mlaC"
                     /locus_tag="ACHJWA_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFO4307133.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQRDAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(50000..50551)
                     /gene="mlaD"
                     /locus_tag="ACHJWA_000467"
     CDS             complement(50000..50551)
                     /gene="mlaD"
                     /locus_tag="ACHJWA_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFO4307134.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(50556..51338)
                     /gene="mlaE"
                     /locus_tag="ACHJWA_000468"
     CDS             complement(50556..51338)
                     /gene="mlaE"
                     /locus_tag="ACHJWA_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFO4307135.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(51346..52155)
                     /gene="mlaF"
                     /locus_tag="ACHJWA_000469"
     CDS             complement(51346..52155)
                     /gene="mlaF"
                     /locus_tag="ACHJWA_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFO4307136.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            52365..53342
                     /gene="yrbG"
                     /locus_tag="ACHJWA_000470"
     CDS             52365..53342
                     /gene="yrbG"
                     /locus_tag="ACHJWA_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFO4307137.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            53356..54342
                     /gene="kdsD"
                     /locus_tag="ACHJWA_000471"
     CDS             53356..54342
                     /gene="kdsD"
                     /locus_tag="ACHJWA_000471"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFO4307138.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHIPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            54363..54929
                     /gene="kdsC"
                     /locus_tag="ACHJWA_000472"
     CDS             54363..54929
                     /gene="kdsC"
                     /locus_tag="ACHJWA_000472"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFO4307139.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            54926..55501
                     /gene="lptC"
                     /locus_tag="ACHJWA_000473"
     CDS             54926..55501
                     /gene="lptC"
                     /locus_tag="ACHJWA_000473"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFO4307140.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            55470..56027
                     /gene="lptA"
                     /locus_tag="ACHJWA_000474"
     CDS             55470..56027
                     /gene="lptA"
                     /locus_tag="ACHJWA_000474"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFO4307141.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            56034..56759
                     /gene="lptB"
                     /locus_tag="ACHJWA_000475"
     CDS             56034..56759
                     /gene="lptB"
                     /locus_tag="ACHJWA_000475"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFO4307142.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            56807..58240
                     /gene="rpoN"
                     /locus_tag="ACHJWA_000476"
     CDS             56807..58240
                     /gene="rpoN"
                     /locus_tag="ACHJWA_000476"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFO4307143.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            58263..58550
                     /gene="hpf"
                     /locus_tag="ACHJWA_000477"
     CDS             58263..58550
                     /gene="hpf"
                     /locus_tag="ACHJWA_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFO4307144.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            58668..59159
                     /gene="ptsN"
                     /locus_tag="ACHJWA_000478"
     CDS             58668..59159
                     /gene="ptsN"
                     /locus_tag="ACHJWA_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFO4307145.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTKGTP
                     DEA"
     gene            59205..60059
                     /gene="rapZ"
                     /locus_tag="ACHJWA_000479"
     CDS             59205..60059
                     /gene="rapZ"
                     /locus_tag="ACHJWA_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFO4307146.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            60056..60328
                     /gene="npr"
                     /locus_tag="ACHJWA_000480"
     CDS             60056..60328
                     /gene="npr"
                     /locus_tag="ACHJWA_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFO4307147.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            60542..61174
                     /gene="yrbL"
                     /locus_tag="ACHJWA_000481"
     CDS             60542..61174
                     /gene="yrbL"
                     /locus_tag="ACHJWA_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFO4307148.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDVAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALASREA"
     gene            complement(61171..61899)
                     /gene="mtgA"
                     /locus_tag="ACHJWA_000482"
     CDS             complement(61171..61899)
                     /gene="mtgA"
                     /locus_tag="ACHJWA_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFO4307149.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDRVSMDQISPWMGLAVIAAEDQTFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSAPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(61896..62549)
                     /gene="elbB"
                     /locus_tag="ACHJWA_000483"
     CDS             complement(61896..62549)
                     /gene="elbB"
                     /locus_tag="ACHJWA_000483"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFO4307150.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGRECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(62779..65115)
                     /gene="arcB"
                     /locus_tag="ACHJWA_000484"
     CDS             complement(62779..65115)
                     /gene="arcB"
                     /locus_tag="ACHJWA_000484"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFO4307151.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVNVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(65211..66140)
                     /gene="yhcC"
                     /locus_tag="ACHJWA_000485"
     CDS             complement(65211..66140)
                     /gene="yhcC"
                     /locus_tag="ACHJWA_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFO4307152.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            66814..71274
                     /gene="gltB"
                     /locus_tag="ACHJWA_000486"
     CDS             66814..71274
                     /gene="gltB"
                     /locus_tag="ACHJWA_000486"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFO4307153.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNEI
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHNDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTET
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            71287..72705
                     /gene="gltD"
                     /locus_tag="ACHJWA_000487"
     CDS             71287..72705
                     /gene="gltD"
                     /locus_tag="ACHJWA_000487"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFO4307154.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVQAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TREEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHSMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            72889..73936
                     /gene="yhcG"
                     /locus_tag="ACHJWA_000488"
                     /pseudo
     CDS             72889..73936
                     /gene="yhcG"
                     /locus_tag="ACHJWA_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhcG family protein"
     gene            complement(73996..74460)
                     /gene="nanQ"
                     /locus_tag="ACHJWA_000489"
     CDS             complement(73996..74460)
                     /gene="nanQ"
                     /locus_tag="ACHJWA_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFO4307155.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLKGEERILFGTAGTARQCEEFHHEDDYQ
                     LCSAIENEQTINLKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(74457..75332)
                     /gene="nanK"
                     /locus_tag="ACHJWA_000490"
     CDS             complement(74457..75332)
                     /gene="nanK"
                     /locus_tag="ACHJWA_000490"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFO4307156.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLGQLTDLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADAKTIFMRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(75329..76018)
                     /gene="nanE"
                     /locus_tag="ACHJWA_000491"
     CDS             complement(75329..76018)
                     /gene="nanE"
                     /locus_tag="ACHJWA_000491"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFO4307157.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLTLVKRLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(76066..77556)
                     /gene="nanT"
                     /locus_tag="ACHJWA_000492"
     CDS             complement(76066..77556)
                     /gene="nanT"
                     /locus_tag="ACHJWA_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFO4307158.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCTAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(77665..78558)
                     /gene="nanA"
                     /locus_tag="ACHJWA_000493"
     CDS             complement(77665..78558)
                     /gene="nanA"
                     /locus_tag="ACHJWA_000493"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFO4307159.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(78680..79471)
                     /gene="nanR"
                     /locus_tag="ACHJWA_000494"
     CDS             complement(78680..79471)
                     /gene="nanR"
                     /locus_tag="ACHJWA_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFO4307160.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            79851..81207
                     /gene="dcuC"
                     /locus_tag="ACHJWA_000495"
                     /pseudo
     CDS             79851..81207
                     /gene="dcuC"
                     /locus_tag="ACHJWA_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
     gene            complement(81250..81747)
                     /gene="sspB"
                     /locus_tag="ACHJWA_000496"
     CDS             complement(81250..81747)
                     /gene="sspB"
                     /locus_tag="ACHJWA_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFO4307161.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(81753..82391)
                     /gene="sspA"
                     /locus_tag="ACHJWA_000497"
     CDS             complement(81753..82391)
                     /gene="sspA"
                     /locus_tag="ACHJWA_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFO4307162.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(82786..83178)
                     /gene="rpsI"
                     /locus_tag="ACHJWA_000498"
     CDS             complement(82786..83178)
                     /gene="rpsI"
                     /locus_tag="ACHJWA_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFO4307163.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(83194..83622)
                     /gene="rplM"
                     /locus_tag="ACHJWA_000499"
     CDS             complement(83194..83622)
                     /gene="rplM"
                     /locus_tag="ACHJWA_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFO4307164.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(83841..84968)
                     /gene="zapE"
                     /locus_tag="ACHJWA_000500"
     CDS             complement(83841..84968)
                     /gene="zapE"
                     /locus_tag="ACHJWA_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFO4307165.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGGKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            85162..85560
                     /gene="zapG"
                     /locus_tag="ACHJWA_000501"
     CDS             85162..85560
                     /gene="zapG"
                     /locus_tag="ACHJWA_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFO4307166.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            85714..87081
                     /gene="degQ"
                     /locus_tag="ACHJWA_000502"
     CDS             85714..87081
                     /gene="degQ"
                     /locus_tag="ACHJWA_000502"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFO4307167.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKTGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            87171..88238
                     /gene="degS"
                     /locus_tag="ACHJWA_000503"
     CDS             87171..88238
                     /gene="degS"
                     /locus_tag="ACHJWA_000503"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFO4307168.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(88302..89240)
                     /gene="mdh"
                     /locus_tag="ACHJWA_000504"
     CDS             complement(88302..89240)
                     /gene="mdh"
                     /locus_tag="ACHJWA_000504"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO4307169.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            89675..90145
                     /gene="argR"
                     /locus_tag="ACHJWA_000505"
     CDS             89675..90145
                     /gene="argR"
                     /locus_tag="ACHJWA_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFO4307170.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT        22301 a        24240 c        22410 g        21396 t
ORIGIN      
        1 acaaggataa ggttataaat gaataaagtt acaaaaacag ctattgcggg cttacttgcg
       61 cttttcgcag gtaatgccgc tgcaaccgat ggtgaaatag tttttgatgg cgagatttta
      121 aaatccgctt gtgaaatcaa tgactctgat aagaaaattg aagttgctct tggtcactat
      181 aatgccgaac agtttcgttc tgtaggcgat cgtagcccaa aaattccatt cactattcct
      241 ttagttaact gcccagtgac tgggtgggag cacgataacg gcaacgttga agcgtctttc
      301 cgtttatggc tggaaacgcg ggataatggt accgtcccta acttccctaa cttggcaaaa
      361 gtgggctctt ttgctggcac agcagcaaca ggtgtgggta tccgtattga cgatgcggaa
      421 agcggaaacc ttatgccact aaatgctatg ggcaatgata acacagttta tcagatccct
      481 gcggactccg ctggaattgt caatgttgac ctcatcgctt actacgtatc aaccgttgaa
      541 gcatccgaaa tcaccccagg ggaagcagac gctgtcgtta acgtaacgct ggattaccgt
      601 taatacgtta cggcgttatc tgacctttca gataacgccc ttttccttcc tatttctcgt
      661 tgtatcaggt tgaaaaataa ctatgtcaaa acgaacattc gcgatgatat ttaccttgtt
      721 gtgtagcttc tgtatcggcc aggcgcttgc agggggtatc gttttacagc gaacgcgagt
      781 gatctatgat gccagccgca aagaggctgc gttacctgtc gcaaacaaag gcgcagaaac
      841 accttattta ctgcaatcat gggtagataa tatagatggt acaagccgtg ccccatttat
      901 tataacccca ccgctaattc gtcttgaagc tggcgatgat tcatcactgc gaattattaa
      961 aacagctgat aacctgcctg aaaataaaga gtcgctgttc tacattaatg ttcgtgccat
     1021 tccagcaaag aaaaaatcag atgatgttaa tgctaacgag ttgacgctgg tatttaaaac
     1081 acggatcaaa atgttttatc gccccgcaca cctgaaggga cgggtaagcg atgcgtggaa
     1141 atcactggaa tttaaacgta gtgatcattc actcaatata tataacccaa ctgaatatta
     1201 cgtcgtattt gccggactag cagtcgataa agccgatctc acaagcaaaa ttgaatatat
     1261 cgcgcccgga gaacataaac agttaccact tccagcatct ggcggaaaga acgtgaaatg
     1321 ggctgcgatc aatgattatg gcggcagttc cgggacagaa actcgtccac tgcaataaca
     1381 aatataaaaa cacaggtcat cagggaatgc cacaacgaca ccaccaggga cataaacgca
     1441 caccgaaaca gttggcgctc attatcaaac gctgtttgcc gatggtgctc actggcagcg
     1501 gcatgctttg cactaccgct aacgccgaag agtattattt cgaccccatt atgctggaaa
     1561 ccacaaaaag tggtatgcaa acaaccgatc tgtcacgttt ttcaaaaaaa tacgcacaac
     1621 taccaggaac ttatcaggtt gatatctggc tgaataaaaa gaaggtttca cagaaaaaaa
     1681 ttacatttac cgccaatgca gagcaacttc tgcagccaca gtttacggta gaacaactac
     1741 gtgagctggg tattaaggtg gatgaaatcc cggcgctggc tgaaaaagat gacgatagcg
     1801 tgatcaactc gcttgaacaa atcattcccg gtacagctgc tgaatttgat ttcaatcatc
     1861 agcgacttaa tttgagcatt ccccaaattg cactgtaccg tgatgcaaga ggttacgtct
     1921 ccccttctcg ttgggacgat ggtataccaa cgctgtttac caactactcg tttacaggtt
     1981 ctgataaccg ttaccgccag ggcaatcgta gccaacgaca gtacctaaat atgcaaaatg
     2041 gtgccaattt tggcccctgg cgattacgta actattctac gtggacacgc aacgatcagg
     2101 cgtcaagctg gaacactatc agtagttatt tacaacgtga tatcaaggcg ttgaagtctc
     2161 agttgcttct gggagaaagc gccaccagcg gcagtatttt ttccagctac aactttactg
     2221 gcgtgcaact cgcttccgac gataatatgt tgccaaacag ccagcgcgga tttgccccaa
     2281 cggtacgcgg tatcgcaaac agtagtgcaa tcgtgactat caggcaaaat ggttatgtga
     2341 tctatcaaag caacgtgcca gcgggtgcct ttgaaattaa cgatctctac ccctcttcca
     2401 acagcggcga tttagaagtc acgattgaag aaagtgacgg tacgcaacgt cgctttatcc
     2461 agccttattc ttcattaccc atgatgcagc gacctgggca tctaaaatat agcgcgaccg
     2521 ctggacgcta tcgcgctgat gcaaacagtg atagcaagga acccgaattt gctgaagcca
     2581 cggcaatata tggtttgaat aatactttta cgctgtatgg cggcctgctc ggttctgaag
     2641 attattatgc gctggggatc ggtatcggcg gcacacttgg cgcactgggc gcgttgtcga
     2701 tggatatcaa cagagctgac acccaattcg ataaccagca ctcttttcat ggctatcaat
     2761 ggcgtacgca gtacatcaaa gatatcccgg aaaccaacac caatatcgct gtcagctact
     2821 atcgctatac caacgatggc tattttagtt ttgatgaagc caatacccgc aattgggact
     2881 ataacagtcg ccaaaaaagt gaaattcaat tcaacatcag ccagacaata tttgatgggg
     2941 taagtctgta tgcctccggt tcacagcaag actattgggg caataacgag aaaaacagga
     3001 atatctctgt tggggtttcc ggccagcaat ggggaattgg ttacagcctg aattatcaat
     3061 acagccgcta cactgatcaa aataatgacc gcgcactctc tttgaatctc agtattccgt
     3121 tagaacgctg gttaccgcgt agccgggttt cctatcagat gaccagccag aaagatcgcc
     3181 caacccaaca tgaaatgcgt cttgatggct cactgctgga tgatggtcgc ctgagctata
     3241 gtctggaaca aagtctggat gacgataaca accataacag tagcgtgaac gccagttacc
     3301 gttcacctta tggaaccttc agtgccggat acagttacgg taatgacagt agccaataca
     3361 attacggcgt taccggcggc gtggttatcc atcctcatgg tgtgacgctc tcgcaatatc
     3421 tgggcaacgc ttttgcgctt attgatgcta acggggcatc tggcgtgagg atacaaaact
     3481 atccggggat tgctactgat ccctttggct atgcagtggt tccttatctc acaacttatc
     3541 aggaaaaccg tctctcggta gatactacgc agctgcccga taacgtcgat cttgaacaaa
     3601 caacacagtt tgtggtgccc aacagaggtg caatggtagc ggcgcgtttc aacgccaata
     3661 tcggttatcg cgtacttgtt acagtcagcg atcgcaacgg taaaccgttg ccctttggcg
     3721 ctcttgccag caacgatgat acggggcaac aaagtatcgt cgatgagggc ggcatactat
     3781 atctctctgg gatatcgagt aaatcacaaa gctggactgt acgctgggga aatcaggcag
     3841 atcaacaatg tcagtttgct tttagtacac cggattcaga accaacaacc tctgtattac
     3901 aaggcacagc gcagtgccat taaggataaa aaaatgaaaa gagcgcctct tataacagga
     3961 cttttgttga tatccacatc ctgcgcttat gcctcctcag aagggtgtgg agctgacagc
     4021 actagcggtg cgacaaatta cagcagtgtg gttgatgatg ttacggtgaa ccagacagat
     4081 aacgtgacag gacgggagtt tacctctgca acgctaagta gcactaactg gcaatacgcc
     4141 tgttcctgct ctgcgggtaa ggcagttaaa cttgtctata tggtcagccc cgtacttacc
     4201 accactggac atcagacagg atattacaaa ctcaatgaca gcctggatat taaaaccatg
     4261 ttattaaggt taattttgga ttcatcaatg gtcagaagtt taccacccgc aatgccatgc
     4321 cagacggttt tactccagta gactttgata tcacttatga ctgtggtgat acttcaaaga
     4381 ttaaaaactc gttgcaaatg cgcatcgacg gtacaactgg ggtagtagac cagtacaacc
     4441 tggtcgccag acgaagaagt tcagacaatg cgcccgatgt cggtattcgt attgaaaatc
     4501 tcggcggcgg agttgcaaat attccttttc agaacggtat ccttcccgtt gatccttccg
     4561 ggcatggcac cgtcaatatg cgcgcctggc cagttaatct ggtcggtggt gagctggaaa
     4621 caggaaaatt tcagggcaca gccaccatta ccgtcatcgt gcggtaaaag aagacgttgc
     4681 gttttgatta acgcaacgct tcggcactct tacccctgct gctccagcgc atacttatac
     4741 agcgcatttt tcttcacgcc gtgaatttct gcggccagcg ccgccgcttt tttcagcggc
     4801 agttctgcct gtagcagcgc cagcgtgcgc agggcatcgg cgggtaagtc ttcttcctgt
     4861 gctttatgac cttcgacaat cagcaccatt tcgcctttgc gacggttttc atcttccttt
     4921 acccacgcca gcagctcgcc aacgggcgcg ccgtgaatgg tttcccaggt tttggtcagc
     4981 tcacgcgcca gaaccacgta gcgggattcg cctaataccg caacgatatc ttccaggcta
     5041 tctaacagac ggtgggtaga ttcataaaaa atcagcgtgc gcggctccgc ttcaatggct
     5101 tttagcgcat cacggcggcc ttttgatttg gcaggtaaaa agccttcgta acagaaacgg
     5161 tcagagggta aacccgcggc gcttaacgca gtgatagcag cacacggtcc gggtagcggc
     5221 accacacgaa tccccgcttc acggcaggta cgcaccagat ggtagccagg atcgttaatt
     5281 agcggcgttc cggcatcgga aaccagcgca atgttttgcc cctcttgcag cttcgccagc
     5341 agcgtttcgg ctttttgttg ttcgttatgg tcgtgcagcg caaacaaccg ggcattaatc
     5401 ccaaaatgtt gcagcaataa accggtgtga cgagtatcct cggcggcaat cagatcaacg
     5461 gcctgtaata cctctaacgc acgctgggtg atatccgcca gattgccgat tggcgtcggt
     5521 acaatgtaaa gctggccctg agaattatcc gccgattggt gttgtttcat tgtgtcgtcc
     5581 gtattgccga tttaatattg agcattgcgt aaaaaaaata tcactggata cattatggta
     5641 ccctcaacat tttctcgttt gaaagccgcg cgttgtctgc ctgttgttct ggcagccctg
     5701 attttcgtcg gttgtggcac ccatactccc gatcagtcca ctgcttatat gcagggcacg
     5761 gcgcaggctg attctgcctt ttatcttcag cagatgcagc aaagctctga tgataccagg
     5821 atcaactggc aattactcgc cattcgtgca ctggtgaaag aaggtaaaac cgggcaggcg
     5881 gttgagttgt ttaaccaact accgcaagaa ctgaacgatt ctcagcgtcg cgagaaaaca
     5941 ctgctggcgg tagagattaa actggcgcag aaagattttg ctggcgcgca aaacttgctg
     6001 gcgaaaatta cacccgccga tttagaacaa aaccagcaag cccgttactg gcaggcaaaa
     6061 atcgatgcca gccaggggcg tccttccatt gatttactgc gcgcgttaat tgctcaggaa
     6121 ccgctgcttg gcgcgaaaga aaaacagcag aatattgatg ccacctggca ggcgctctcc
     6181 tccatgactc aggaacaggc gaatacgctg gtgatcaacg ccgacgaaaa tattctgcaa
     6241 ggctggctgg atctgcagcg cgtctggttt gataaccgta acgatcccga catgatgaaa
     6301 gccgggatcg ccgactggca gaaacgttat ccgaacaatc cgggcgcgaa aatgctgcca
     6361 acgcagttgg ttaacgtaaa agcgtttaaa ccagcctcga ccaacaaaat cgccctgctg
     6421 ttgccactga atggccaggc agcggtattt ggtcgcacta ttcagcaagg ctttgaagcg
     6481 gcgaaaaata tcggcactca gccagtggcg gctcaggtag ctgccgcacc tgccgcagac
     6541 gtagcagaac aacctcagcc gcaaactgcg gatggcgttg ccagcccggc acaagcctcg
     6601 gttagcgatc tgaccggtga tcagcctgcg gcccagccgg tgcctgtaag cgccccggcg
     6661 acaagcaccg cagcggtaag cgcacccgca aatccatccg cagagctgaa aatctacgat
     6721 acctcatcac aaccacttag ccagatctta agccaggttc agcaggatgg cgcgagtatt
     6781 gtggttggtc cgttgctgaa aaataacgtt gaagagttgc tgaagagcaa cactccgctg
     6841 aacgtactag cactgaacca gccggagaat atcgaaaatc gcgtcaatat ttgttacttc
     6901 gcgctttcac cggaagacga agcgcgcgat gcagcgcgtc atattcgtga ccagggtaaa
     6961 caagcgccgc tggtgctgat cccacgcagt tcattgggcg atcgcgtagc caatgcgttt
     7021 gcgcaagagt ggcagaaatt gggcggcggc accgttctgc aacaaaaatt tggttccacc
     7081 agcgaattac gcgcgggtgt taacggcggt tctggtattg ctttaacggg taccccgatt
     7141 actcccagag cgacaaccga ctccggcatg acgaccaaca atccaacgct gcaaaccacg
     7201 ccaaccgatg accagttcac caataatggc ggtcgtgtcg atgcggtgta cattgtggca
     7261 acgccgggtg aaatcgcttt tattaaaccg atgatcgcca tgcgtaacgg tagccagagc
     7321 ggtgcaacgc tgtacgccag ctcccgcagt gcgcaaggga ccgctggccc ggatttccgt
     7381 ctggagatgg aaggcttgca gtacagcgaa atcccgatgc tggcgggcgg taatctgccg
     7441 ttaatgcagc aggcactcag cgcggtgaat aacgattatt cactggctcg catgtatgcg
     7501 atgggcgtcg atgcctggtc gctggcaaat catttctcac aaatgcgcca ggttcagggt
     7561 tttgaaatca acggtaatac cggaagcctg acggctaacc cggattgcgt gattaacagg
     7621 aagttatcat ggctacagta ccaacaaggt caggtagtcc ccgccagtta accaccaaac
     7681 agaccggcga tgcgtgggaa gtacaagcgc gtcgctggct ggaaggcaaa ggactgcgat
     7741 ttgtcgccgc taacgtgaac gagcgtggcg gcgagatcga tctgataatg cgtgaaggcc
     7801 ggaccaccgt ttttgtcgag gtgcgttacc gccgttctgc gctttatggc ggcgcggcag
     7861 ccagtgtgac ccgcagcaaa caacacaaat tattacagac tgcccgcttg tggctcgcgc
     7921 gtcataatgg gagttttgat actgtggatt gccggttcga tgtggtagcc ttcaccggga
     7981 atgaggttga gtggattaag gataccttta atgaccactc ataattaagg tttaaggatt
     8041 agcgtgcaag aaagaattaa agcttgcttc actgaaagca ttcaaactca aattgcggcg
     8101 gcagaggcgc ttccggatgc catctcccgt gcggccatga cgctggttca gtctctgctc
     8161 aatggcaaca aaatcctctg ttgtggtaat ggaacttccg ctgccaatgc acagcatttt
     8221 gctgccagca tgatcaaccg tttcgaaacg gagcggccca gcttacctgc cattgcacta
     8281 aataccgata atgttgtctt aacggcgatt gccaacgatc gcttacatga tgaagtgtat
     8341 gcaaaacagg tgcgggcgct ggggcatgcg ggagatgtat tgttagccat ttccacccgt
     8401 ggcaacagcc gcgatatcgt aaaagcagta gaagctgccg ttacgcgtga tatgaccatt
     8461 gtggcattga ccggttatga cggcggtgaa cttgcaggtt tgttagggcc acaggatgtg
     8521 gagatccgca tcccttcgca tcgcagtgct cgcattcagg aaatgcatat gctgacggta
     8581 aattgcctgt gcgatctgat cgataacacg cttttccctc accaggatga ttaaggagaa
     8641 tacatgaagg cattatcgcc aatcgcagtc cttatttccg cgctgctgtt gcaaggttgt
     8701 gttgccgctg ccgtagtggg tactgctgct gtgggtacca aagccgcaac tgacccacgc
     8761 agtgtcggca cccaggtgga cgatggtacc ctggaagtgc gcgtgaacag cgcattgtcg
     8821 aaagacgaac agattaagaa agaagcgcgc attaatgtaa cggcctatca gggcaaagtg
     8881 ctgctggttg ggcagtcacc aaacgctgaa ctttcggctc gcgccaaaca gattgctatg
     8941 ggcgtagacg gtgccaacga agtgtataac gagattcgtc agggccagcc gattggtctg
     9001 ggcgaagcgt ctaacgatac gtggatcacc accaaagtgc gttcgcagct cttaaccagc
     9061 gacctggtga aatcgtccaa cgtgaaagtg accaccgaaa acggtgaagt gttcctgatg
     9121 gggctggtga ctgaacgtga agcgaaagcg gcggcagata ttgccagccg ggtgagcggc
     9181 gtgaagcggg taactacggc gtttacgttt attaaatagc agccccttgt aatgcctgat
     9241 gcgacgcttg ccgcgtctta tcaggcctac aacgatgcaa accgtaggtc ggataaggcg
     9301 tttacgccgc atccgacatg ttttccctca aatcagaata acagcgccag cccgccgaca
     9361 atcacgcctg acactgccac catcgccccc gtcagccata aggctttcgc cgggaacgct
     9421 ttgcgcagca taatcagtga cggcaaactc accgccggga gcgtcatcaa caatgccagc
     9481 gccggagcag ttcccatacc tgccagcatc atcgtttgta caatcggaat ttctgctgcc
     9541 gtgggaatga caaacaagca ccctgctacc gccatcgcca ccacccacat caggctgtta
     9601 tcgacagcac catcggcatg ggggaataac cagacgcgag cggcacccaa caccagtact
     9661 gcaaggatgt aaaccgggat cgtactccag aaaagcgtcc atagcgccct gccccagcga
     9721 ctaaaaaatc cgccctgtgc ttccggtatg tcaatttcga ccggtgcttg cgtttgcggt
     9781 gtttcacgca cccatttttg caccagcgtc gcaatcagca acaccatcac cagcccggcc
     9841 accagacgaa tcgccgcaaa accccagccg aggacaaacc ccataaacac cagcgtcgcc
     9901 gggtttaaca ccggattgcc catccagaat gccagcgcac cgcccatcga cacctgttgg
     9961 cgacgcattc ccgccgcgac cggagccgca cagcaggtac acatcatgcc tggcagcgaa
    10021 aacagcgttc ccagcagcgt gccgcgaaag cgcgattgcc caagcgtacg caacaaccag
    10081 tcacgcggga tcagcacctg aatcaacgaa ccgaggatca cccccagcac cgccgctttc
    10141 cataccgcga ggaaatagat catcgcgtaa tccaacgccg cctgccatgg attagcatcc
    10201 gcctgcgcaa ggatagattt accgatactg tgggtttcgg cagcagtaaa cgctttgccg
    10261 tagtaaggtt gccatttcac ataccagaga ccggcaatga caacgagaaa gaaaagcgcg
    10321 ggtttccacc actgaatggg cgttgccgcc tgagatgaag actgaccagt catagcattc
    10381 cccaggaata agttatgata attgagcgcg tgaatattac gctcactatc aattcttggg
    10441 aataattatt cagctctttt gcgtaattct gaagagctta aaatcgtcac accttcatgc
    10501 tcgggccgca tcgcctctgc cagcatgacc cgcgccacgt ctctcgcatc aatggatttc
    10561 cagttaccgg gtaacaaacg gaacagcggc gcaaaaagcg tttcgttcat ccgctgtttg
    10621 ctacgatcgc ccagtaacat cgacgggcga gcaatggtca atttcggcca gttctgggcg
    10681 attaatgctt cttccatctc ccctttgacg cggttataga aaaacggcga gtgggcattg
    10741 gcacccatag cactgaccac cagcatatgc tgcgcgccca gtcgccgccc ggttaatgcg
    10801 gtatccacta ccagcgtgta atcggcatga ataaacgcct ctttgctccc cgcttctcgc
    10861 cgcgtggtgc cgagacaaca aaacacaatg tcgatgggat cggtgacctg cgccagcgcg
    10921 tcagtcagtt gcggatcatg gggattaaat accccgggca tatcgcccaa cggacgtcgc
    10981 gtcggcgcgg ctatggcgtt aactttcggt tcgttaatca acatccgcag caggtgaccg
    11041 cccaccagcc ccgttgcgcc tgtaatcagt acctgactca tcttcgctcc tttacagaat
    11101 tgtctgcctt gcgctccacg gctcatgcac caggcttaat agaccatgag gtaattatcc
    11161 ccgattgtgg gaaattcgcc tcatccaatg caacaacgcg gaggaagcat gagtaagaaa
    11221 attgccgttt taatcactga tgaatttgag gattcagaat ttacttcacc cgcagacgag
    11281 ttccgtaaag ccggacacga agtgattacc attgaaaaac aagcgggtaa aacggtgaaa
    11341 ggcaaaaaag gagaagccag cgtgaccatc gataaatcca tcgatgaagt gacgccagcg
    11401 gagtttgatg ccctgttgct accgggcggt cattcaccgg attatctgcg tggcgacaac
    11461 cgttttgtca cctttacccg tgattttgtg aatagcggca aacctgtttt cgccatctgt
    11521 cacggtccgc agctgctgat cagcgccgat gtgattcggg ggcgcaaact gaccgcggtt
    11581 aaaccgatca ttattgatgt taaaaacgcg ggcgcggaat tttacgatca ggaagtcgtg
    11641 gtcgataaag atcagctggt taccagccgg acaccggacg atctgccagc gtttaaccgc
    11701 gaggcgttac gcctgctcgg tgcctgagcc gcgtagccaa atcatttttt taccaaagcc
    11761 cagcgtgttg tcggtgaatt tgatttcatc gggccggatt tcccacaccg gggccgacag
    11821 cattctggca accggaaagc gacgattgta cgccttgcgc gcgaggtcgc tttcctcacc
    11881 ttccagcctg cggatctcac ctttaaactg cacgccgcga attaacgcta ccgttttcgg
    11941 ctgaccgttt accgttccgg caaccgcagc ctgcggccca ctcatctgcg cgtggcgcgt
    12001 tttttcttcc gtcaaaatgt agaaggcgac tttctgcaca tcaaacagat aaaaggcatt
    12061 ggcgcaccag agttcccctt cctgctgcac acaccaggtg acaacatgtt gttttgccag
    12121 ccaacggctg atggcggtga gtgtttccat tgctgttctc ttttatactg tgggcctgac
    12181 cttaacatgc gacgcagacg atgacaccct ggtttcttta cttgatccgt accgccgaca
    12241 ataagcttta taccgggatc accacggatg tcgaacgccg ctatcagcag caccaaagcg
    12301 gcaaaggggc gaaagcactg cgcgggaaag gagaactgac gctggcgttt tctgcgccag
    12361 tgggcgatcg ttcgctggcg ttacgggcgg aatatcgcgt taagcaactg accaaacggc
    12421 aaaaagagcg tctggtagcg gaaggcgcag ggtttgcaaa gctgttaagc agtctgcaaa
    12481 ccccggagat taaaagcgat tgaaatgctc gtgatactca accagaccag ttacgccatt
    12541 caacgcgtca tccgctaaac gatgtacctg gaaggcgctt tcagtgcccg gccagcggca
    12601 acgcagatcg tgatgcgccg ccagttcaaa gccgaaacgg ctgtatagcg ccggatcgcc
    12661 cagcgtcacc actgcggcat agccgaactc attaagcgaa tcgagtcctt catagaccag
    12721 ttggcgtgcc agcccctgtc cacggtattt ttcatctacc gccagcggtg ccatgccgac
    12781 ccattgcagg tcttcgccct gcacatcaac cggactaaat gccacatagc caatgacctg
    12841 accttcgtca tctgtcgcca ccagccccag cgtcagaaag ccatcttcac gcagatcgtg
    12901 aaccagcttc gcttccgcat cactttcgaa tgaacgacgc agcagggcat caatacccgg
    12961 cgcatcaatg ggaatttcta ctcgaattag catggttcac ctaccgatgt ctgtttggtt
    13021 tcaggcgcgg ttttcattcc cgcctcaaca aaatccgcca gttgcagcag catcatgcgt
    13081 aaggctttcg gcatttgctc cagttcaatg gcgtccatca ggtttttcac atacagcccc
    13141 agctccgtat cgccttcaat caccagccga cgctggaaga agagcgtatc cggatcttgt
    13201 ttacgcgccg caatcatcag cagatcgctg gcgtcggcac taaaactcac atcagcttgc
    13261 gcgttctggc tgaccaccag tttgccattc accaccgagg taaaccattg caggtcaata
    13321 tcacgcacat gaatacttaa ccagcggcct tcaagaaact ccagctcacc atcatccagc
    13381 gcctggcgga attgccagct taagacctgc tcaagaacct ggcgttttag cgcaaatggc
    13441 gtcagtttta ccggtacact caacagagat ggccccaaat gcacaatacg ggaacgcagt
    13501 ttatccaaca cgagttttac tccctgtttc aacaatcatc ctattttgcc atatcagaaa
    13561 aataacatag cggtataaat caacaattcc atatgaaatt gctgctacca ccaatacaac
    13621 tttaactgcc ttaaatcaaa aattgtcgca gcaaggttaa ctaaaatccc agttcgttaa
    13681 catttttgcg ttttgatagc gcaaccttca ggaaaaatta tggagctgct ctgccctgcc
    13741 ggaaatctcc cggcgcttaa ggcggccatc gaaaacggcg cagatgctgt ttatatcggg
    13801 ctaaaagatg ataccaatgc ccgtcacttc gccggcctta actttaccga gaaaaaattg
    13861 caggaagcgg tgagttttgt ccatcaacat cgccgcaaac ttcacatcgc gattaacact
    13921 tttgcgcatc cggacggtta cgcccgttgg cagcgtgccg tggatatggc ggcgcagctg
    13981 ggtgccgacg cgctgatcct cgccgacctc gccatgctag agtacgccgc cgagcgttac
    14041 ccgcatattg agcgtcatgt gtcagtgcag gcttcggcga ccaatgaaga ggcgattaac
    14101 ttttatcatc gccattttga cgttgctcgc gtggtgctgc cgcgcgtgtt gtcgattcat
    14161 caggtgaaac aactggcacg ggtcacacct gtcccgctgg aagtctttgc tttcggcagc
    14221 ctgtgcatta tgtcggaagg tcgttgctat ctgtcgtcgt atctgacggg tgagtcgccc
    14281 aacaccgtgg gcgcgtgttc tccggcccgt ttcgtgcgct ggcagcaaac gccgcagggg
    14341 ctggaatccc gcctgaacga agtgctgatc gaccgttatc aggacggcga aaacgcaggt
    14401 tatccgacgc tatgtaaagg gcgttatctg gtggacggcg agcgctatca cgcgctggaa
    14461 gaaccaacca gtctcaatac cctggaactg ctgccggagt taatggcggc gaatattgct
    14521 tcggtgaaaa ttgaaggccg ccaacgtagc ccggcgtatg tcagccaggt ggcgaaagtc
    14581 tggcgtcagg ctatcgaccg ttgtaaggcc gatccgcaaa acttcgtacc gcaaagcgcg
    14641 tggatggaga cgctcgggtc gatgtccgaa ggcacgcaga ccacccttgg cgcgtatcac
    14701 cgtaaatggc agtgagaaga gcaatgaaat attccttagg gccagtgctg tggtactggc
    14761 caaaagagac gctggaagat ttttatcagc aggccgccac cagcagcgcc gacgtgattt
    14821 atcttggcga agcggtttgc agcaagcgcc gggcaaccaa agttggcgac tggctggaga
    14881 tggcaaaatc gctcgccgga agtggtaagc agattgtgct ctccacgctg gcgctggtgc
    14941 aggcatcatc tgaactgggc gaactgaaac gctatgttga gaacggtgag tttctgattg
    15001 aagccagcga tctcggcgtg gtgaatatgt gcgctgaacg caaactgccg ttcgtcgccg
    15061 gacacgcgct gaactgctat aacgcggtga cgctgaaaat attgctcaaa cagggcatga
    15121 tgcgctggtg tatgccggtg gagctttccc gcgactggct ggtgaatctg cttaatcagt
    15181 gcgatgagct gggcattcgc aaccagtttg aagttgaagt cctgagctac ggtcatctac
    15241 cgctggccta ctccgctcgc tgctttaccg cgcgttcgga agaccgcccg aaagatgagt
    15301 gtgaaacctg ctgcattaag tatccgaacg ggcgcaacgt gctgtcgcag gaaaaccaac
    15361 aagtgtttgt actcaatggc attcagacca tgagcggcta cgtttacaac ctcggtaacg
    15421 agctggcatc catgcagggc ctggttgatg tggtccgcct gtcaccgcag ggtactgaca
    15481 ctttcgcgat gctcgacgcc ttccgcgcta atgaaaatgg cgctgcacca ctgccgttga
    15541 cggcgaacag cgactgtaac ggctactggc ggcggctggc aggactggag ctgcaagcct
    15601 aagtaaatag ctcactttgt taacaacttt aactactctt taatgcagta ttaaagatta
    15661 atcggtaaca aagtgagctg ttatgactga taaaaccatt gcgttttcgc tactcgatct
    15721 ggcccccatt cccgaaggtt cttcagcgcg agatgcattc tcccactctc tcgatctcgc
    15781 ccgtctggct gaaaagcgcg gctatcatcg ctactggctg gcagaacacc acaatatgac
    15841 tggcattgcc agtgctgcca cgtcggtatt gattggctat ctggcggcga ataccaccac
    15901 gctgcatctg gggtctggcg gcgtgatgtt gcctaaccac tcaccgttgg tcattgcaga
    15961 acagttcggc acgctcaata cactctatcc ggggcgaatc gatttggggc tgggtcgtgc
    16021 tccgggtagt gaccagcgaa ccatgatggc gctacgtcgt catatgagcg gggatattga
    16081 taatttcccc cgcgatgtcg cggagctggt ggcctggttt gacgcccgcg atcccaatcc
    16141 gcatgtgcgc ccggtaccag gctacggcga gcaaatcccc gtgtggttgt taggctccag
    16201 cctttacagc gcgcaactgg cggcgcagct tggtctgccg tttgcgtttg cctcacactt
    16261 cgcgccggat atgttgttcc aggcgctgca tctttatcgc agcaacttca aaccgtcggc
    16321 acgactggaa aaaccatacg cgatggtgtg catcaatatt atcgctgccg acagcaaccg
    16381 cgacgctgaa tttctgttta cctcaatgca gcaagccttt gtgaagctgc gccgtggcga
    16441 aaccgggcaa ctgccgccgc cgattcaaaa tatggatcag ttctggtcac cgtctgagca
    16501 gtatggcgtg caacaggcgc tgagtatgtc gctggtgggc gataaagcga aagtgcgtca
    16561 tggtttgcag tcgatcctgc gcgaaaccga cgccgatgag attatggtta acgggcagat
    16621 tttcgaccac caggcgcgcc tgcattcgtt tgagctggcg atggatgtta aggaagagtt
    16681 gttgggatag tgtgtcttaa cgcgggaagc cttatccgag ctggcaacgc tgtcccacgt
    16741 aggcctgata agcgaagcgc atcaggcatt gtgtaggcag cagaaatgtc ggataaggcc
    16801 cgctgattac tgatacaccg gcagcaaatt aaagctcgat aagatatgga ccagtgcgtt
    16861 gccaacgcca aataccagaa tcagcgtaat catcggcttg ccgccccaga cgcggaattt
    16921 cgggctgcca aagcgtttac gcgatgcgcg ggccagcagt gccggaacaa ttgccgccca
    16981 gatggtagct gctaaaccag cataaccaat ggcgtacagg aatccgttcg ggaacagcag
    17041 accacccaca acaggagggg caaaggtcag caatgccgtt ttcaagcggc ccacagccga
    17101 gtcgtcgaaa ccaaacagat ctgccagata gtcaaacaaa cccagcgtta cgccgaggaa
    17161 cgaactcgct accgcaaagt ttgagaacac gaccagcagc agatccagac tacggctgtt
    17221 cagtacgccg cttaacgcct gtaccagcac atcaatatta ccgcccttct ctgcaatatc
    17281 gataaactcc ggacgcggga tgttacccat cgtcgccagc aaccagatgg tatacagcgc
    17341 cagcgccatc agcgtaccgt acaccagaca tttcacgatg gttttcggat ctttgccgta
    17401 atacttcatc aggcttggca cgttaccgtg ataaccaaac gatgccagac agaacggcaa
    17461 ggtcatcaac agatacggtg cataagacgc attgctttca gcgacgttga acaatgtcgc
    17521 aggctgcaca tgccccagca ggctaccgaa ggtgaggaag aaggtaatga ctttcgcccc
    17581 caatacaatc gctgtcatgc gactgacggc tttagtgctc aaccacacca caaacgctac
    17641 cagcaatgca aaaccaaaac ccgccgcccg tgccgggacg tttagtgaca tctctgcgaa
    17701 ggtgtgatgc agaatcgaac cactggcaga aatataggca taggtcagga tatagagcac
    17761 aaaggcaatg gaaatgccgt tgaccacgtt ccagcctttg cccagcaaat ctttggtgat
    17821 ggtgtcaaaa ctcgaaccga ttctgtaatt caggttagct tccagaatca tcaagccgga
    17881 atgcagcata cagaaccagg taaagatcag cgccgccatt gaccagaaaa accacgcccc
    17941 ggacatgacc actggcagag aaaacatccc tgcgccaata atggtgccgc cgataatcac
    18001 cacgccgcca agcagcgacg gtgacgtttg ggtggtggtt agtgttgcca tgagggcttc
    18061 tctccagtga aaaatagtgc gactgcgttg ttatgtattg cactgtacca gtacgcgagt
    18121 acaaaagaca gaaaaaaagc cccgatggtt caaatcgggg ctgtatatat tattttacag
    18181 actgtgttcg ctgttcagcg atgattacgc atcaccaccg aaacgacgac gaccggtaga
    18241 gtcatcacga cgcggagcgc ggccttcacg acgttcgccg ctaaaacgac gaccatcacc
    18301 acgaccacct tcacggcgtt caccgctgaa gttacgacca ccttcacgac gctcgccact
    18361 gaaaccacga ccaccgccac gacgctctcc gccagtatgc ggctgtgcat cgcccagtaa
    18421 ttgcatattc atcggcttgt tcagaatgcg agtacgtgta aagtgttgca gcacttcacc
    18481 cggcatacct ttcggcagtt cgatggtgga gtgagaagca aacagcttaa tgttaccgat
    18541 gtaacggctg ctgatgtcgc cttcgttagc aatcgcacca acgatatgac gaacttcaac
    18601 accatcatcg cggcccactt caatacggta caactgcatg tcaccaacat cacgacgttc
    18661 acgacgcgga cgatcttcac ggtcgccacg cggaccacgg tcgttacgat cgcgcggacc
    18721 acggtcatca cggtcacgga attcacgttt cggacgcatc ggcgcatctg gcggaacaat
    18781 cagagtacgt tcaccctgtg ccattttcag cagtgcggcg gccagagttt cgagatccag
    18841 ctcttcacct tctgcagtcg gctgaatttt gctcagcagt gcgcggtatt gatccagatc
    18901 gctgctttcc agctgctgct gtactttagc ggcgaatttt tccagacggc gtttgcccag
    18961 cagttctgcg ttcggcagtt ctacttccgg aatagtcagc ttcatagtac gttcaatgtt
    19021 gcgcagcaga cgacgctcgc ggttctcaac gaacagcagc gcgcggccag cacgacccgc
    19081 acgaccggta cgaccgatac ggtgaacgta agactcagaa tccatcggga tatcgtagtt
    19141 aaccaccagg ctgatacgct caacgtccag gccacgggct gcaacgtcgg tcgcaatcag
    19201 gatgtccaga cgaccatctt tcaggcgttc cagtgtctgc tcacgcagcg cctggttcat
    19261 gtcaccgttc agcgcggcgc tgttgtagcc gttacgctca agagcttcag ccacttccag
    19321 agtcgcattt ttggtacgaa cgaagataat cgccgcatca aaatcttccg cttccaggaa
    19381 acgtaccagt gcttcgtttt tacgcatacc ccagacagtc caatagctct ggctgatgtc
    19441 aggacgggta gtcacgctgg actgaatgcg cacttcctgc ggctctttca taaagcggcg
    19501 ggtaatgcga cgaatcgctt ccggcatggt tgcagagaac agagcggtct gatgaccttc
    19561 cgggatctgc gccataatag tttcaacgtc ttcgatgaag cccatgcgca gcatttcgtc
    19621 agcttcatcc agaaccagac cgctcagttt agagaggtcc agagtgccac gtttcaggtg
    19681 gtccagcaga cggcccggag taccgacaac gatctgcggc ccctgacgca gggcgcgtaa
    19741 ttgcacgtca taacgctggc cgccgtacag ggcaaccaca tttacgccgc gcatgtgttt
    19801 agagaaatcc gtcattgctt cagcaacctg taccgccagt tcgcgggtcg gtgctagcac
    19861 cagaatctgt ggtgctttca gctcaggatc aagattttgc agcagaggta aagagaatgc
    19921 tgcagttttt ccgctccccg tctgggccat acccagtacg tcgcggccat tcagcagatg
    19981 tggaatacac tccgcctgaa ttggagatgg tttttcgtaa cccagatcgt taagggcttc
    20041 aaggatagga gccttcaggc ccagatctgc aaaagtggtt tcgaattcag ccatgtagta
    20101 cgtgtgcctc aaaattaatg gcggccagtc tacataactc atcatgaaat tgatcagcaa
    20161 ttttcattga aaagtgtgaa ccggctcaaa gtaggtgtat taacgaacaa caacgccctc
    20221 acccgttaag gtgatggcaa tcaaaaaaga ttacgggctg atgtgtacgt cagctattgc
    20281 tggtccgatt ctgccaggtc atcttggtcc tggcccagga gcgataattc caacaatgcg
    20341 tatcggtgct caacaaagtt atgaacgttg ttggcaaccg ccagtttgaa cagtgccgtg
    20401 gcgctgtcca aatcccccag acttaggtag tacttaccta aatagaagtt ggtttcactg
    20461 agatgctcag cgagcgaggt gttatccgtt gcgtccgcct tgagcctttc cattaacgtt
    20521 tgttcgctaa tgttgcccag gtagaactcg acaatgttcc atccccactg ttccttatcc
    20581 gatttttcga agtgctgttt caacacttct ttagcctgct tctcatcgag cttctgctcg
    20641 gcgagataaa gccacagact acggaaagga tcattgggat cgtcttgata aaacgccagc
    20701 agatcatctt gcgctaactt gtcacgaccg ccgtaatata atgcgatccc gcgattcaag
    20761 tgcgcgtagt tgtaagttgg atcaagctca agtacagaat caaacgcttc ataggcagca
    20821 tcaaaattgc ctgcctgcgt taaatatatg cctaagtaat tgaatacttc aggcatatcc
    20881 ggtcggattg ccagcgcttg cgaaaaatcg ttacgcgcta atgccctcag accgagacta
    20941 tcatacaaca ctccgcgctc atataaaagc tgtgcgcgtt cgtcatcggt taaagcccga
    21001 ctggcaagga tttgttccat acgtgccaga atcacttcct gctgtaaagt cggttgcaat
    21061 ggtaccgcga ggacttcact tttacgccag gaagtattac tgcatcctgc aagcgtaagt
    21121 gctgtcgcaa cgaaacacca gcgcaaaaaa ggcttcattt cccactcccg aagaccacgg
    21181 ttgaatgaac gtcctgttcc cggttgctaa caaggcgtcc tgcccggtta aaagcccccc
    21241 gccgcagcgg agggcaaatg gcaaccttac tcgccctgtt cagcagccgg agcttccggt
    21301 gctgcagcag gttgagactg ctcagtcgct tctttaatgc tcagacggat acggccctgg
    21361 cgatcaactt ccagaacttt caccggtact tcctgaccca tctgcaggta atcggtcact
    21421 ttctcaacgc gtttgtcagc gatttgagag atgtggacca gaccttcttt accgccgccg
    21481 atggcaacaa atgcgccgaa gtcaacgata cgggtcactt taccagtgta gacgcggccc
    21541 acttcaattt ctgcagtgat ctcttcgata cgacgaatag cgtgtttcgc tttctcgcca
    21601 tcggtcgctg cgatcttcac agtaccgtca tcttcgattt cgatggtggt gccagtttct
    21661 tcggtcaggg cacggatcac agagccgcct ttaccgataa cgtctttgat cttgtccggg
    21721 ttgatcttga tggtatggat acgcggagcg aactcagaga tatcgccacg tggcgcgttg
    21781 atcgcctgtt ccattacacc caggatgtgc agacgcgcac ctttagcctg gttcagtgca
    21841 acctgcatga tctctttggt gataccttca attttgatat ccatctgcag tgcagagata
    21901 ccgtcgcggg aacccgcaac tttgaagtcc atatcgccca ggtgatcttc gtcgcccaaa
    21961 atgtcagaca gtacaacgta gttgtcgcct tctttcacca gacccattgc gatacccgca
    22021 acggcagctt tgattggcac acctgcgtcc atcagcgcca gagacgcacc gcacacggaa
    22081 gccatagaag aagaaccgtt ggattcagtg atttcagaca caacacgtac ggtgtacggg
    22141 aatttgtcca tatccggcat tactgccagc acgccgcgct tcgccagacg accgtgacca
    22201 atttcacgac gcttcggaga accgaccatg ccggtttcgc ctacggagta cggagggaag
    22261 ttgtagtgga acaggaaggt atcggtacgt tcgcccatca gttcatcaag aacctgcgcg
    22321 tcacgagcag tacccagcgt tgcggtaacc agcgcctgcg tttcaccacg ggtgaacagc
    22381 gcagaaccgt gagtacgcgg cagcacgcca gtacgcacat ccagaccacg gatcatatct
    22441 ttttcacgac catcgatacg cggttcgcct gccagtacgc ggctacgaac aacgtttttc
    22501 tcgatcgcgt gcagaatttc acccagttcg ttttcgtcca gggtttcgtc ttcagcaagc
    22561 agcgtcgcga tggtttcaga tttgatgaca tcaacctgcg cataacgctc ttgtttgtcg
    22621 gtgatgcggt aagcatcgct cagacgagct tcagccagtg cagcaacgcg cgcgttcagc
    22681 gcttcgttta ccggctccgg ctgccagtcc caacgcggtt taccggcttc tttcaccagt
    22741 tcattgatgt tctgaataac aacctgctgt tgttcatgac cgaacactac tgcgcccagc
    22801 atctggtctt cgctcagcag ttcagcttca gattcaacca tcagtacagc ggcttcagta
    22861 cccgcaacaa ccagatccag tttgctctct ttcagctcgt cctgagtcgg gttcagtacg
    22921 tactggtcat tgatgtaacc tacgcgggca gcaccaatcg ggccattgaa cggaatacca
    22981 gacagagaca gtgctgcgga agcaccaatc atcgcgacga tatccgggtt aacctgcggg
    23041 ttaacagaaa ccacggtggc gataacctga acttcgttga cgaagccttc cgggaacagc
    23101 gggcgaatcg ggcggtcaat cagacgcgcg atcagggttt cgccttcgct tggacgacct
    23161 tcacgacgga agaagctacc cgggatacga ccagcagcgt aggtacgctc ctgatagtta
    23221 acggtcagtg ggaagaagtc ctgacctggt ttggcttttt tctggccaac aacggtaaca
    23281 aataccgcgg tgtcatccat gctaaccata acagcggcag tagcctgacg agccatcatg
    23341 ccggtttcca gagtcacggt gtgttggccg tactggaatt tacgaacgat cggattaagc
    23401 aatgtaatat cctttctctt tcttagacag taccttacgg cactggtgtt aatacccgat
    23461 cttctgcgca tcctcgcgac taatgacaac cctaacccag ctctatgtgg gtaaagcctc
    23521 tcattagccg cgcgaacctc tgcaacggaa gatcattcat agcaacaata cattagtttc
    23581 cagtgaattg ctgccgtcag cttgaaaaaa ggggccactc aggccccctt ttctgaaact
    23641 cgcaagaatt agcgacgcag acccaggcgc tcgatgagcc gggtgtaacg tgctacgtct
    23701 ttacgtttca ggtagtcgag cagtttacga cgctgagaaa ccatgcgcag cagaccacga
    23761 cggctgtggt gatctttttt gtgctctgca aagtggccct gcaggtggtt gatctgtgca
    23821 gtcagcagtg ctacctgaac ttcggtagaa ccggtgtcgt ttgcgtcacg accaaactca
    23881 gaaacgattt tagctgttgc ttcagtactt agagacattt taaaactcca aagtatatag
    23941 aatgaaagga cgccgatctc taattcagcg atcccagtgt acgttacgca aagtgttaaa
    24001 caatttacgc gacgttaagc ggcagtattc tactcgtagc gacctgttat cgcaagacgg
    24061 ttaacattac gccgggtatt caaccaccag acgacgaggc gcaacgcggc cttcatcgtc
    24121 aatttcgccc ataccgataa acttgccgtt ctcaccttcc gtaacgcgaa ccagtccttc
    24181 cagcggcgcg ccagatgtac gaaccgggtt accatttttg aagtaaacag aagacgttaa
    24241 cggaagattc accaccgggt agtccgaagc tggactgtcc attggcatca gtaatggatc
    24301 aagcaactcc gcagccggaa tatcctgctg ttcggcttgt tcgaccagtt cacgcaggtg
    24361 ctccagggtc accatccgtt caaccggata tttacttacc gccagacgac gcaggtaaat
    24421 cacatgcgcg ccacagccga gtttttcacc caggtcatca atgatggtgc ggatataagt
    24481 gccttttgag cagtgaattt ccagctccag ctcattgcct tcatggcgaa taaacagcaa
    24541 ttcataaacg gtaattggac gcgcttcacg cggaacttca atgccctgac gcgcatattc
    24601 gtacagtttt ttgccctggt atttcagtgc tgaatacatc gatgggatct gttcgatatc
    24661 gccacggaaa gtatccagtg ccgccgccag ttgttctgca ctaaaggtta ccggacgctc
    24721 ttcaacgatt tgtccgtcgg cgtcagaagt atcggtacgc tgtccaagac gcgcaatgac
    24781 ccgatagcgt ttgtcggagt ccagcagata ctgggaaaac ttcgtcgctt ccccgaggca
    24841 aatcggcaac atgccggtcg ccagcgggtc cagcgcaccg gtatgcccgg cacgattggc
    24901 gttatatata cgtttcactt tttgcagcgc atcgttgctg gacataccct gcggtttatc
    24961 cagcaacaaa acgccgttaa tgtcgcgacc gcgacgacga ggacgactca ttagtcctcc
    25021 ttgctgtcgt ccgggttaac acgacgttct tcgtcatgtt tgaccacgct ggtcaccagg
    25081 tttgacatac gcatcccttc aaccagagag ttgtcgtaga agaaggtcag ttccggcacg
    25141 atacgcaggc gcatcgcttt acccagcagg ctgcggatga aaccagaagc ttcctgcaac
    25201 gctttgatgc ccgctttaac cgcgtcttca tctttgtcgt tgaggaaagt cacatacact
    25261 ttggcatacg ccaggtcgcg agacatttcg acaccggaaa cggtggtcat catgcccaga
    25321 cgaggatctt taatttcacg ctgcaggatg agagcgatct ctttttgcat ttcctgcgct
    25381 acgcgctgcg ggcgaccaaa ttctttcgcc ataataaatt ctcctgacaa aaaaggggct
    25441 gttagcccct ttttaaaaat taatttcagg tggaagggct gttcacgttg gcctgataag
    25501 acgcgccagc gtcacatcag gcaatccatg ccggatgcgg cgtaaacgcc ttatcccgca
    25561 tggaacccta aaaaccttaa gcgatggtac gttggatctc gatgatttcg aatacttcga
    25621 tcacatcgcc agtgcggacg tcgttgtagt tcttaacgcc gataccacat tccataccgt
    25681 tacggacttc gttaacgtca tctttgaagc ggcgcaggga ctccagctcg ccttcataga
    25741 taaccacgtt gtcacgcaga acgcggatcg ggttgtgacg tttaaccaca ccttcggtaa
    25801 ccatacagcc tgcgatggca ccaaatttcg gcgatttgaa cacgtcacga acttccgcca
    25861 gaccgataat ctgctgtttc agttccggag acagcatacc gctcatcgcc gctttcactt
    25921 cgtcgatcag gttatagatg acggagtagt aacgcagatc caggctttcc gcttcaatca
    25981 ctttacgtgc agaggcatca gcacgtacgt tgaagccaac caggatggcg ttggacgccg
    26041 cagccaaggt ggcgtcggtt tcggtgatac cacctacgcc agaaccgatg atcttcactt
    26101 taacttcgtc agtagacagt ttcagcaagg agtcggagat cgcttcgaca gaaccctgta
    26161 cgtctgcctt caggacgata ttcacttcgt gaacttcgcc ttcggtcatg ttggcgaaca
    26221 tgttctcgag tttagatttc tgctgacgcg ccagtttaac ttcgcggaat ttaccctgac
    26281 gatagagtgc aacttcacgc gctttcttct cgtcacgtac aacggtaact tcatcacccg
    26341 cagccggaac accggacaag ccgaggattt ccaccggaat ggacggaccc gcttccagca
    26401 cttcctgacc cagttcgtta cgcatcgcac gaacacgacc gtattcgaag ccacacagaa
    26461 cgatatcgcc cttgtgcaga gtaccttcac gtaccagaac ggtagcaacc ggaccacgac
    26521 ctttatcgag gaaggattcg ataaccgcac cgctcgccat acctttacgt accgctttca
    26581 gctccagaac ttccgcctgc agcaggatag cgtccagcag ttcatcgata ccggtacccg
    26641 ctttcgcaga tacgtgtacg aactggcttt caccgcccca ctcttccggc aggatgccgt
    26701 actgggagag ttcgttctta acgcgatccg gatcggcttc tggtttatcg atcttgttca
    26761 ctgcaaccac caccggtacc tgcgccgctt tcgcgtgctg gattgcttcg atggtctgcg
    26821 gcattacgcc gtcgtcggca gcaacaacca ggactacgat gtcagttgcc tgcgcaccac
    26881 gtgcacgcat tgaagtaaac gcggcgtgcc ccggggtgtc caggaaggtg atcatgccgt
    26941 tttcagtttc aacgtggtat gcaccaatgt gctgggtaat gccgcccgct tcgccagagg
    27001 ccactttcgt tgaacgaatg tagtccagca gagaggtttt accgtggtca acgtgaccca
    27061 tgatggtcac aaccggagcg cgcggttcag ccgcagctcc cgtgtcacgg tcgctcatta
    27121 ccgcctcttc cagctcgttt tcacgacgca ggataacttt gtggcccatc tcttcagcaa
    27181 ccagctgtgc ggtttcctga tcgataacct ggttgatggt tgccattgcg cccagtttca
    27241 tcatcgcttt gatgacctga gagcctttaa ccgccatctt gttcgccagt tcgccaacgg
    27301 tgatagtttc gccgatcaca acgtcacggt taacggcctg agcaggcttc tggaagcctt
    27361 gctgcagcga agaaccttta cgttttccgc ccttaccgcc acgtactgct gcgcgtgctt
    27421 cttcacgatc agcttttgat tcagcgtgtt tgttgccttt cttcggacgc gctgctttcg
    27481 cgttacgacc acggccacgg ccgccttcga cttcacgatc gctttcgtct tctgcctggc
    27541 gagcatgttg agaagtagtg acgtgataat cgctggaatc ctcagtcggt tccgcgttat
    27601 cagtccattt gttttcttcc gccatacgac gtgcttcttc agcaacgcga cgtgcttctt
    27661 cttcgagttt acgacgcgct tcttcttcgg ctttacgctt gagctctgca gcttcctgct
    27721 cacggcgggc tttttcagcc tgggcgtttt tagtcatatc gtcttgttga ttgctcactt
    27781 tgtctttttc cgcagcttca cgtttcgctt gttcagcagc ttcacgctta gcttgttctg
    27841 cggcctcacg ttcagctttt tgttgcgcct cgcgtttagc cgattcttct gcctcacgac
    27901 gggcttgctc ttccgcttca cgctgcgctt gctcttccgc tgcaaggcgt tcagcctctt
    27961 gcggatcgcg tttcacaaag gtgcgtttct tgcggacttc gatttgtacc gatttgcttt
    28021 ttccaccggt accaggaatg ttaagggtgc tgcgtgtttt acgttgcagc gtcaatttgt
    28081 ccgggcctga atttttctga ttcaggtggt caatcaaagt ctgtttctct tgtgcagaca
    28141 cagagtcgtc agcagacttc cggatacctg catcagcaaa ttgctgtacc aggcgttcca
    28201 cggaggtctg tcgctctgcg gccagcgttt taatcgttac atctgtcatg ctgttccttc
    28261 ctgctacagt ttattacgct tcgtcaccga accagcaaat attacgggca gccataatca
    28321 gtgctccggc tttttcgtcg gtcaaccctt cgatatcagc cagatcatca atgccctgtt
    28381 cggcgagatc ttccagcgta caaacgccac gggcggccag tttgaatgcc aaatcacgat
    28441 ctaccccttc aaggttcagc agatcgtcag ccggtttgtt atcaccgagg ctttcttcct
    28501 gggcctgtgc aatggtggcc agtgcatttt tagcacgctc gcgcagtgct tcaacggtcg
    28561 gctcatcaag gccttcgatt tccaacagct ctttcatcgg cacataggcc agttcttcca
    28621 gcgtcgagaa gccttcctct accagaacag tcgcgaagtc ttcgtcgatg tcgagatatt
    28681 tggtgaaggt gtcgatcgct gcgtgcgctt ccgcctgatg cttagcctgc aggtcgtcaa
    28741 cggtcatcac gttgagttcc caaccgctca gctgcgaagc cagacgcacg ttctgaccgt
    28801 tacggccaat cgcctgcgcc aggttaccgg cttcaacggc gatatccatg gtgtgtttat
    28861 cttcatccac cacgatagaa gcaacgtctg ccggtgccat tgcgttaatc acaaactgcg
    28921 ccgggttatc atcccacagg acgatatcga tacgctcgcc gcccagttca gtagaaaccg
    28981 cctgaacacg cgcgccacgc atacctacgc aagcacctac cggatcgata cgtttatcgt
    29041 tggttttcac cgcgattttc gcacgagaac ccgggtcgcg agccgctgct ttaatttcaa
    29101 tcacttcttc gccgatttct ggcacttcaa tacggaacag ttcgatcagc atttccggct
    29161 tggaacgagt gacgaacagt tgcgcgccac gcgcttccgg gcgaacggaa taaagcacgc
    29221 cacgaacgcg gtcgccaggg cggaagtttt cacgcggcag catatcttcg cgcaggatca
    29281 cggcttcagc gttgttgccc agatccagag agatgttgtc gcggtttact tttttcacca
    29341 cgccggtgat gatttcacct tcgtgttcac ggaactgatc aaccaccatc gcacgttcgg
    29401 cttcacgcac tttctgcacg ataacctgtt ttgccgtctg ggtggtgata cggtcaaagg
    29461 taacagactc aatctgatct tcaacgtaat cgcccaggtt caggctttca tcttcataac
    29521 gtgcggcttc aagggtgatt tccttggtcg gctgggtgac ttcatcaaca actaaccagc
    29581 gacggaaggt gtcaaaatca ccgcttttgc gatcgatctg tacgcggacg tcgatctctt
    29641 gttcatattt tttctttgtt gctgtcgcca gcgcgctttc caatgcttcg aaaatcttct
    29701 cgcgaggtag cgccttttca ttggatacgg cttcaactac agccaaaatt tctttgttca
    29761 tcgcgggctt ttcacctcat ccagactatt aaaagtgggg aaccaagttc gccttctgga
    29821 tattactcag cgcgaacact tcatctttac cttcgacggt aactgtgatc atttcaccgt
    29881 ctaccgcttt gataacgccc tgccatttac gacggttttg taccgccata cggagaacca
    29941 gagtcacctc ttctccgaca aaacgggcgt agtgttcagc cgtgaacagt gggcgatcga
    30001 gacccggtga ggagacttcc aggttataag caacggtgat gggatcttca acatccagca
    30061 cagcacttac ctggtggctc acatcagcac aatcatcaac attgatgcca tcttcactat
    30121 caatatagat gcgcagtgtg gatgtgcgac cgcgaataaa ttcgatgcca accagttcaa
    30181 aacccagggc ctcaactggc gcagtaatca tctctgttaa tttttgctct aatgtggaca
    30241 agcccacccc caagacataa aaaaagggcc taaagcccag ttattctgta gtcagataac
    30301 aaaaaacccc gataaatcgg ggctttatat aactgaaccc tataaccgca actgcggtct
    30361 ggagcacttt ccagaaggat tttttcaaat cccactacga aggccgaagt cttcacagta
    30421 tatttgaaaa aggactctaa gggaaagtgg ttgcgggggc cggatttgaa ccgacgacct
    30481 tcgggttatg agcccgacga gctaccaggc tgctccaccc cgcgcctgaa acgtggcaaa
    30541 ttctactcgt tttgggtaaa aaatgcaaat actgctggga tttggtgtac cgagacggga
    30601 cgtaaaatct gcaggcatta tagtgatcca cgccacattt tgtcaacgtt tattgctaat
    30661 cacgtgaatg aatatccagt tcactttcat ttgttgaata cttttgcctt ctcctgctct
    30721 cccttaagcg cattatttta caaaaaacac actaaactct tcctgtctcc gataaaagat
    30781 gattaaatga aaactcattt attttgcata aaaattcagt gagagcggaa atccaggctc
    30841 atcatcagtt aattaagcag ggtgttattt tatgacgacg attctcaagc atctcccggt
    30901 aggtcaacgt attggtatcg ctttttccgg cggtctggac accagtgccg cactgctgtg
    30961 gatgcgacaa aagggagcgg ttccttatgc atatactgca aacctgggcc agccagacga
    31021 agaggattat gatgcgatcc ctcgtcgtgc catggaatac ggcgcggaga acgcacgtct
    31081 gatcgactgc cgcaaacaac tggtggccga aggtattgcc gctattcagt gtggcgcatt
    31141 tcataacacc accggcggcc tgacctattt caacacgacg ccgctgggcc gcgccgtgac
    31201 tggtaccatg ctggttgctg caatgaaaga agatggcgtg aatatctggg gtgacggtag
    31261 cacctataaa ggaaacgata tcgaacgttt ctatcgttat ggtctgctga ccaatgctga
    31321 actgcagatt tacaaaccgt ggcttgatac tgactttatt gatgaactgg gcggtcgtca
    31381 tgaaatgtct gaatttatga ttgcctgcgg tttcgactac aaaatgtctg tcgaaaaagc
    31441 ttactccacg gactccaaca tgcttggtgc aacgcatgaa gcgaaggatc tggaatacct
    31501 caactccagc gtcaaaatcg tcaacccgat tatgggcgtg aaattctggg atgagagcgt
    31561 gaagatcccg gcagaagaag tcacagtacg ctttgagcaa ggtcatccgg tggcgttgaa
    31621 cggtaaaacc tttagcgacg acgtagaaat gatgctggaa gctaaccgca tcggcggtcg
    31681 tcacggcctg ggcatgagcg accagattga aaaccgtatc atcgaagcga aaagccgtgg
    31741 tatttacgaa gctccgggga tggcactgct gcacattgcg tatgaacgcc tgttgaccgg
    31801 tattcacaac gaagacacca ttgagcagta tcacgcgcat ggccgtcagt tgggccgtct
    31861 gctgtaccag gggcgttggt ttgattccca ggcgctgatg ctgcgtgact ctctgcaacg
    31921 ctgggttgcc agccagatca ctggtgaagt taccctggag ctgcgccgtg ggaacgatta
    31981 ttcaatcctg aataccgtct cagagaacct gacctacaag ccagagcgtc tgacgatgga
    32041 aaaaggcgac tcggtgttct cgccagatga tcgtatcggt caactgacca tgcgcaacct
    32101 ggatatcacc gatacccgcg agaaactttt cggttatgcc aaaactggcc tgctttcctc
    32161 ctctgccgct tcaggcgtgc cgcagatgga gaatctggaa aacaaaggcc agtaattcga
    32221 catcaaccct gcccttcagg ggtggggtta ccttgcttat cctctgtaaa ctcctttctg
    32281 agcatcacat aatcgaaaac atcatggttt gcatccacaa ccagtgagtc tcctttatag
    32341 tgtacaatca cttcctcaag cgtttgcggc tgttccggct ttgttacccc catccacgca
    32401 ttaataaggt aattattgta agctgtcgaa aagatgttgt tttccgttgt gcgatccaca
    32461 ccatccccaa gaacggggct ataccagata aacatcggaa catgatatgc ctgctggctg
    32521 gcctccctgc ctccatgaaa atagacgttc ttcttcgtag ggtcacgttc caggccgtgg
    32581 tcggcaaaat acatgaccga ggcgcggcga tcttttaata attcaaaaac ctgacctagc
    32641 aaactatctg tgtaatgaat ggagttgtca tagcaggcat cctgatcgtc ctgcggttga
    32701 aacacggcgc tggattgcgg ataggcgcta caagccggtt catggcttcc atttaaatga
    32761 agaacaatca gtttcttttg ctgcgtattt tgctgtaatg cttgcgataa atgcggcaac
    32821 aacaattcat caaatcctct gacataaact gtttccatgg cacgcatggc gatactggta
    32881 actgctgtac cattctgccg aaaagcggat tgcgagctta gccagaaagt ctgaaatcct
    32941 gcttgattag ccatattaat aatgttgtcg gggtaattat gaatgtcatg gctcaaaaca
    33001 gaatcagcag ttaaagaaag gggaaccgac agcgcagtgt aaggtgcacc gcttattgct
    33061 tgattaaaca gtttgatctg ttttctttgt gcttcgactt gcggtgtcgt agagcgtgta
    33121 tatccataca aagacatatt gtcgacacgt acagactccc ccacaatcaa cacgtaggta
    33181 tcaatacctg tatccctgac tgataattga aaatacggca ctgtgtttgc aattgagagt
    33241 aatctttgat gctctttcgc tgctaaagca aaataattga gattgaaaaa cggcgtatag
    33301 gtagcaaatc gcgacgctag tatatatgga ctgaacgcat ttttattttt ggcatcttta
    33361 taagcaaatt gacaagcgga aaataaactt cccgaaatga caatcagcaa taatattcct
    33421 gtcacttttt ttgtcggcaa ggaaacatca tattttatta ttacagccaa aaaaagaagg
    33481 gataaaaagg caaaagcaca tagataaggg ctatacattc ccagcatttt ggcaacttca
    33541 tccggatcac tctgcagcac actaatcgca aatccatcat tgaaagttgt accaaaggta
    33601 caccatgaat agaggctaat actcatgtct gccgcagtca gtaacacaaa tggtgcggca
    33661 acaagcaggc gaagcggaag ggatgatata cgtttaacaa ccaggatggt cagaacgaag
    33721 aacagcaatc gctgaaccat atgcgctccg gaagaggcga ctaagttcgt aataccaaaa
    33781 acaataacac taagatacaa caaccaatga gatttaaaac ttctagcaaa tttgttgaat
    33841 actgtcatcc agaaatccaa gttcacaacc aatataaaca attatcaaca atgtgcaatt
    33901 ttatatcaga catgtttttc aactctgatc catatcagaa cgaaacactg ttagtttttt
    33961 ctttttaaat gatgcggcca ttgcgttctc agtaattaaa taacattatc aatgcgtctc
    34021 taaacattac gcagaacgta gccatcagta gttatactga caggagcagg atggtttatc
    34081 gagaagtaaa catatccgta ttcgtaccgg aactggagtt gacacaataa agtgccaatt
    34141 atgtcagcag tagggaaaag tttataacga aggcgtaccg gcaccatcgt ttcaaggtac
    34201 cagctacgag taaagcaact ggacgagata cagatacctg acaaccattc ctcagaccag
    34261 gaccaaaacg aaaaaagacg cttttcagcg tctcttttct ggaatttggt accgaggacg
    34321 ggacttgaac ccgtaagccc tattgggcac taccacctca aggtagcgtg tctaccaatt
    34381 ccaccacctc ggcacggaaa ctacttttta gttcgggata tcgctggtcg gcttagccgg
    34441 agcagctggc tgagtttgtt cggttttcgc cggcgcactc aggttttccc attcgctacc
    34501 tttattggtc ttgttgctat tgatgttacc cagcaccaga ctgatgatga agaataacgt
    34561 tgccagcaac gccgtcatgc gggtcatgaa gttaccagaa ccacttgaac caaacagcgt
    34621 agcggaagcg cctgctccga aggaggctcc catatcagcg cctttacctt gctgtagcat
    34681 gatcagacca acaaggccaa ttgccacaat aaggaaaact accaaaagag cttcgtacat
    34741 aatcaacctg ttccttgcgg agttgccgcg taccaatgct tcaaccaata aagcgggagt
    34801 ttataatctt tcccactgaa gcgggtgtga atactaacca aaacgaatga ccttcgcaag
    34861 ggcaatttta tcgcattgta tcaactgcgg aaaaaaacag caaaaccgat gtgttcgccg
    34921 agaaaaaagg cgacaatcgc cgccttttta gccagttatc taacgcttta aacggctttt
    34981 actgcatcgg cgatgcggtg tgcaaattca gtcacctgcg cttcgtcttc gccttccacc
    35041 atcacgcgaa ttaacggttc ggtgccggat ttacgcaaca gcacacgtcc acggctaccc
    35101 agcgccgttt caacttctgc ggtcaccgct ttgactgatt catgctccag cggatcgccg
    35161 ctacctgcgg tgtaacgcac gtttaccagg atttgcggga acattttcat gccgctgcaa
    35221 aggtcgtgca ggctcatatg gttacgcgcc atcgccgcca gcacctgcaa accagcaacg
    35281 atgccgtcac cggtagtggt tttatccagc aggatcacat gaccggaatt ctctgcaccg
    35341 atacgccagc ctttctcctg cattttttcc agtacgtagc ggtcacccac tttcgcgcgc
    35401 gcaaatggaa ttcctaactg tttcagcgcc agttcaagcc ccatgttgct catcaatgta
    35461 cccacagcgc caccacgcag ctggccctga cgaagacctt cacgcgcgat gatatacatg
    35521 atctgatcgc catcgacttt attgccttca tggtcaacca taatcacgcg atcgccgtcg
    35581 ccgtcgaagg caataccgag atccgctttt tcagccagca cacgagcttg gagcgcgcga
    35641 acgtcggtag cccccacttc ggcattgatg tttacaccgt ttggctcaca accgatagcg
    35701 ataacattcg ctcccagttc gcgcagcacg ttcggcgcga tgtgataagt cgcaccgttt
    35761 gcgcaatcca ccacaatctt cagttcactg aggctaagtt cgttcgggaa cgtggctttg
    35821 caaaactcga tatagcgacc cgcagcatca acgatacggc tggctttacc cagttctgcc
    35881 gaatcaacac agctgatctc tttttccatt tccgcttcga tggcttcttc aactgcatcc
    35941 ggcagcttag taccgtcaat agaaaagaat ttaatgccat tatcgtagaa cgggttatgc
    36001 gatgcagata tcacaattcc ggcctctgcg cggaaggtac gcgtcagata agccacggcc
    36061 ggtgttggca tcgggccagt gaagagtgcg gaaaggcccg cagccgccag ccccgcttcc
    36121 agtgctgact ccagcatata gccagaaata cgcgtgtctt taccaataat aatcttacgg
    36181 gagccgtggc gcgccagcac tttacccgcg gcccaaccca gcttaagcac aaaatcaggt
    36241 gtgatcggcg catcccctac acgaccacga atcccatcgg taccgaaata tttacgatta
    36301 ctcatagcgt ttgttttcct ttgcagacag agtagcttcc accactcgca tcgcttctac
    36361 ggtttctttg acgtcatgaa cacgaataat gtgcgcgcct tgcattgcgg caatgaccgc
    36421 acaggccaga ctaccgctca ggcgctcgga cggccccacg ttcagcagct gcccaatcat
    36481 cgattttcgt gacataccca ccaacagcgg caggttgaaa tggtgaaatt cagccaggcg
    36541 cgccagtaat gaatagttat gggagagatt tttaccgaaa ccgaatccgg ggtcgagcaa
    36601 caatttctct tttgcgatac ccgcctgctc gcaacgtgct atttgctcaa taaagtagcg
    36661 attcacttct gcaaagacat cgtcatactt cggggcttcc tgcatggttt ttggatttcc
    36721 ctgcatatgc atcagacaaa ccggtaaacc ggtttctgca gccgcctcca gagcgccagg
    36781 ttcggaaagg gagcggatat cgttaataat gtgagcgcca actttcgctg actcacggat
    36841 gacttctggt ttggatgtat cgaccgagat ccagacttcg aagcgttgag caattgcctc
    36901 aaccacagga ataacacgtt gcaactcttc ttcaacgcta acttccgccg cccctggccg
    36961 cgtggactcg ccaccaacgt caatgatcgt cgcgccagcg ttgatcatca gattcgcatg
    37021 tttcaccgca tctatcagcg agttatgcgt gccaccatcc gaaaaggaat caggcgtgac
    37081 gttgaggatc cccattacgt gaggatggct aaggtccagt gaagtaccct gggcaaagag
    37141 tttcatgatg ttatccctgg tatgaattga taagaaaaaa ccccggagca cgccccgggg
    37201 ttttcggtac aaatacagtc atctgatgcg ggaacttact tgtcgcctaa ctgctctgac
    37261 atggtgttac ccgggttcgg cgtacgcggt tcatcaaccg gacgaggagc ctttggacta
    37321 ccattgtcgc cagcattgtt agaagcgcct ggttcttccc agcccgctgg cggacgtaca
    37381 tcgcgacgtg ccatcaggtc atcaatctgc ggtgcgtcga tagtctcata tttcatgaga
    37441 gcatctttca tcgcatgcag aatatccata ttgtcggtca gaagctgacg cgcacgatta
    37501 tagttacgct caatcagtgc tttcacttcc tggtcgatga tacgtgcagt ttcatcggac
    37561 atatgtttcg ctttcgctac gctacggccg aggaacactt caccttcttc ttccgcgtac
    37621 agcagtggac ccaatttctc agagaagccc cactgagtca ccatgttacg tgccaggttg
    37681 gtcgcaactt taatatcgtt ggacgcaccg gtagaaacat gttccggccc gtagatgatc
    37741 tcttctgcca gacgaccacc gtacagcgta gaaatctggc tttccagttt ctgacggctg
    37801 gcgctgattg cgtcgccctc aggcaagaag aaagtcacac ccagcgcacg accgcgtggg
    37861 ataatcgtca ctttgtgcac cggatcgtgc tccggcacca ggcgaccgat aatcgcatga
    37921 cccgcttcgt ggtaagccgt cgattctttc tgcgcttccg tcatcaccat ggagcgacgt
    37981 tccgcaccca tcatgatttt gtctttcgct ttctcgaact caaccatcga cacaacgcgt
    38041 ttgttgccac gagcagcgaa cagtgccgct tcgttcacca ggttcgccag gtcagcaccg
    38101 gagaaaccag gagtaccacg ggcaatgatt gccgcgtcga tatcgggtgc caatggtacg
    38161 cgacgcatgt gaaccttcag gatctgctca cgaccgcgaa catctggcaa gccaaccaca
    38221 acctgacggt cgaaacggcc aggacgcagc agcgctgggt cgagaacgtc cggacggtta
    38281 gtcgcggcga taacgatgat accttcgtta ccttcgaagc catccatctc aaccagcatc
    38341 tggttcagag tctgttcacg ttcatcgtga ccaccgccca gacctgcgcc acgctggcgg
    38401 cctacggcgt cgatttcatc gataaagatg atgcacggtg ccgctttctt cgcctgttcg
    38461 aacatgtcac gaacacggga tgcacccaca ccgacgaaca tttctacgaa gtcagaaccg
    38521 gagatagtaa agaacggaac tttcgcttcg cctgcaatcg ctttcgccag cagcgtttta
    38581 ccggtacccg gaggaccgac catcaggacg cctttcggga tcttaccgcc gagtttctgg
    38641 aagcggctcg gctcgcgcag atactcaacc agttcagcaa cttcttcttt tgcttcgtcg
    38701 cagcccgcaa cgtcagcaaa ggtcgttttg atctgatctt ccgtcagcat gcgcgcttta
    38761 ctcttaccaa acgacatggc acctttgcca ccgccgccct gcatttgacg catgaagaag
    38821 atccagacac caatcagcaa cagcatcggg aaccaggaga tgaagataga agccagcagg
    38881 ctgggttctt caggcggttc accgacaacc ttgacgttct tggtcaacag gttatccagt
    38941 aatttcggat cctgaaccgg aatgtaagtg gtataacggt tactatcttt cttggtaacg
    39001 ttgatttcac gtccgttgat acgcgcttca cgaacctggt cgttattgac ctcttgtagg
    39061 aaggtagagt aatccacctt acggccatta gactcgctgg gcccaaagct ctggaatact
    39121 gacatcagca caacggcaat gaccagccag agtattaggt ttttcgccat gtcactcaag
    39181 ggattaacct cttattacaa ctgtgttaaa aacagcgtca ggatactcta tatccagcat
    39241 ctttcaaact ttcgtctgaa atctcccggt tagggtttac gcccggtcgc tacaatatac
    39301 acttcccgcg aacgtgcacg agaagagtcc ggcttacgaa ctttgacctt cgtaaacagg
    39361 gagcgaattt cccttagata ctcatcgaaa ccttcgccct ggaacacctt cactacaaaa
    39421 ctgccacctg gcgctaatac atcacgacac atttctagcg ccagttccac cagatacatg
    39481 gcacggggga tatccaccgc cggtgttccg ctcatgtttg gtgccatatc ggacatgaca
    39541 acctggactt tgctgtcgcc aacgcgctcc agcagtgctt tcatcaccag ttcatcacga
    39601 aaatcgccct gaagaaagtc cacaccaacg ataggatcca taggtaaaag atcgcaagcg
    39661 atgatgcggc ctttgccgcc aatttgggtg accacatatt gtgaccaacc acccggagca
    39721 gcaccaaggt cgacaaccgt cattcccggt ttaaagagtt tgtcactttg ctgtatttca
    39781 tcaagtttaa accaggcacg ggaacgtaac ccctttttct gtgcctgttg aacatattta
    39841 tcgctaaagt gttcctgaag ccagcggctg gagctggcag aacgcttctt acctgtcatt
    39901 taactttccc atggggataa ctcatcgtaa ccaattgcgt aaatttttac gcgcctattt
    39961 ggtgatatat gggagatggc ggtagaatga cccgttttca atcccaacgt aagcaaaaaa
    40021 tacgatgaat ctgagtacta aacaaaaaca gcacctgaaa ggtctggcac atccgctcaa
    40081 gccagttgtt ctgcttggca gtaatggttt gaccgaaggg gtgctggccg agattgaaca
    40141 agcgttagag caccatgaac tcatcaaggt gaaaatcgcc accgaagatc gcgaaactaa
    40201 aaccttgatc gtggaagcta tcgtgcgcga aaccggcgcc tgtaatgtac aggtcatcgg
    40261 taaaacgctg gtgctttatc gcccaactaa agaacgtaaa atctcgctgc cacgctaaga
    40321 ttatcctaaa gttacacaca tttgctgtgt aaaacgaggg gttttccgca ggcaggagag
    40381 caaaatgcca cgctctgttc gttgataaaa ggccgctatg cggccttttt cctttcttta
    40441 caatacatca acatcttgag tattgggtaa ttcttacagg tattccacct taattacttc
    40501 aaattctact tcgccgcccg gcgttttgat gaccacaaca tcatcttctt ctttgccgat
    40561 caggccacga gcaataggtg agtttacaga aatcaggttt tgtttaaagt cagcttcgtc
    40621 atcgccaacg atgcgataag tctgttcttc gtcagaatcg agattcagca ccgttacggt
    40681 agcaccaaaa ataacgcgcc cattgttggg cattttggtg acatcaatca cctgcgcgtt
    40741 cgacagcttg gcttcgatgt ctttgatacg gccttcgcag aagccctgct gttcacgagc
    40801 tgcgtggtat tcggcgtttt ctttcaggtc gccatgctca cgcgcttccg cgatagcagc
    40861 aatgatttca ggacggcgca cagatttcag aaaatccagc tcttcgcgta atttttcagc
    40921 gccgcgtaag gtcatcggaa tagcttgcat ttgttatacc tcttgaatat tcctgatggg
    40981 gcaagtgttc acccaccccg gctgttcggc ctgcccggca taactcctca ccgggaccag
    41041 aagcaaaaaa ataccgaccc gggtacaagt cccaggtcag ctacaattca cattttgata
    41101 gtcattttac cctgaagttc ccgaagggtc atcgtttact ttatagggcg ttgcgccgta
    41161 gtatgacggc tcgattccag gttgttagcg cgagattatg cgattttcca gatttatcat
    41221 cggattgacc agctgtatag cgttcagtgt tcaggccgca aatgttgatg agtacattac
    41281 tcaactcccc gctggtgcca accttgccct gatggtgcaa aaagtcggcg cgtcggcccc
    41341 cgctattgat taccacagtc agcagatggc gctgcccgcc agtacccaga aagtgattac
    41401 tgcgctggcg gcgttgattc aactcggccc cgattttcgt ttcaccacga cgcttgaaac
    41461 caaaggcaat gtggaaaacg gcgtacttaa gggtgactta gtggcgcgat ttggtgccga
    41521 tccgacgtta aaacgtcaag atattcgcaa tatggtcgcg actttgaaaa aatctggcgt
    41581 caaccaaatc gatggcaatg tgttgataga tacctccatt ttcgccagcc acgataaagc
    41641 ccccggctgg ccatggaatg acatgacaca atgctttagc gctccgcctg ccgtcgccat
    41701 agttgaccgc aactgtttct ccgtctcgct ctacagtgcc ccaaaacctg gtgatatggc
    41761 ttttatacgc gtggcatctt attaccccgt tacgatgttc agccaggtac gcaccctccc
    41821 ccgtggttct gccgaagcgc aatactgcga actggatgtg gtgccaggcg acctgaaccg
    41881 ctttacgctg acgggatgcc tgccacaacg ttctgagccg ctcccgttgg cttttgccgt
    41941 gcaggatgga gccagctatg ccggtgcaat tctgaaagat gagttaaaac aggcgggtat
    42001 cacctggagc ggaacactgc tgcgccagac tcaggttaac gaacctggaa cggtagttgc
    42061 cagtaaacag tcggccccgc tgcacgatct gcttaagatt atgctgaaaa agtcggacaa
    42121 catgatcgcc gatacggttt tccgcatgat aggccatgcg cgcttcaatg tgcctggaac
    42181 atggcgggcc gggtcggacg ctgtgcgtca gatcctgcgc cagcaagccg gtgtcgatat
    42241 tggaaacacc attattgccg atggttcagg gctttcgcgg cataacctga ttgcccccgc
    42301 caccatgatg caggtgctgc aatacattgc ccaacacgac aatgaactta actttatctc
    42361 catgctgccg ctagcgggct atgacggctc tttgcagtac cgtgcaggtc tgcatcaggc
    42421 gggcgtggat ggaaaagtct cagcgaaaac cggttcgttg cagggggtat ataacctggc
    42481 gggattcatt accacagcga gcgggcaacg aatggcgttt gtacaatatc tttctggcta
    42541 tgcagtagaa ccggcggatc agcgtaatcg ccgtattccg ttagtgcgtt ttgaaagccg
    42601 tttgtataaa gatatttatc agaacaatta gtcaaaaaga aaccccggca catggccggg
    42661 gttttgctga ttaacgcttg taaatgaact caacgccttc ttcgtcgtct tcgtcccagt
    42721 cgtcatccca gtcctcatca tcctcttcag caatctcttc aagctgctgg cgatgataat
    42781 catcccacat gaattcgact ttctctggct gtttcgcttc ttcagcctgc acgaccgggt
    42841 tttcaatgat aaaggtcatc acatcccagc agagatcttt cacgcccagt ccactcgccg
    42901 cagagatcag ataatattta tcttcccagc ccagcgcctc tgcgatcgct ttcgctttct
    42961 cttcggcttc taccttatcc agcagatcga tcttgttgaa gactaaccaa cgcggtttcg
    43021 ccgccagatc ctggctgtat ttttccagct cgctgataat aatacgcgcg ttttcaaccg
    43081 gatcggtgcc gtcaatcgga tcgatatcga tgaggtgcaa caggacgcgg caacgttcca
    43141 agtgcttcag gaagcgaatg cccagacctg cgccttccgc agcgccttca atcagtcctg
    43201 gaatatcggc aacaacgaag ctcttttcgt tgtccattcg taccacaccc agacttggca
    43261 ccagagtggt aaacggataa tccgccactt tcggtttagc cgccgatacc gcacgaataa
    43321 aggtcgattt acccgcgttt ggcatcccca acataccgac gtcagccagc agcatcagct
    43381 ccagcagcag ctcacgctta tcgcccggcg taccgttagt tttctgccgt ggtgtacggt
    43441 taactgacga tttgaaacgg gtattgccca gaccgtgcca gccgccctta gcaaccagca
    43501 gacgctgacc gtgtttggtc atatcgccca tggtttcacc agtaccctgg tcgattacac
    43561 gcgtacctac cggcacttta attgtgacgt ctttaccgcg cttaccggta cagtcgcggc
    43621 ttgcgccatt ctgaccgcgc tctgcacgga aagatttttc aaaacgataa tcgataagcg
    43681 tgttcaggtt ctcgtcggct tccatccata cgtcaccacc atcaccgccg tcgccgccat
    43741 ccgggccgcc tttcggaata tacttttcgc ggcggaagct cacgcaacca ttaccgccat
    43801 cacctgcaac gaccagaatc gatgcttcat caacaaactt cattttattc tccgtaaatc
    43861 attcgcctgc gcggggttgc gataccaccg ttgtatgctt acgtaatccg ccccaaatac
    43921 gatgaccaat ggcggaatac atcgcgcccg caaccacgac aaacgcaccg agataaccta
    43981 aaaggtttaa catcggtctg gcgaagaaat cgggccaggc cagtgataaa agatctgaaa
    44041 aaaacagcgt aaacagtggg gtgagcgtga tgatcgcgct cacctgcgct gcctgccagc
    44101 gagccatcgc ttccgccagg gcgccatatc ctaccaaggt attcagtccg caaaaaatta
    44161 aacatgcgag ctgccagtgg ctaagctgcg ctatcactcc aggctttgcc agagggaaga
    44221 gcgcaattgt acataaagtg tacagtaaaa acaggatctg cggtgaggcc agccgacgca
    44281 ataaaacctt ttgcgccacg ccataactca cccaaaccgt cgcagcaccg accccaaaga
    44341 taactcccca ggtgtaatcg gttagctttg taaatatctc gaccagactg gtgttaaaaa
    44401 acatcaccag gccgctcagg agcatcaatg cccctacaac ctgagtgctg cgcattttct
    44461 ctttcaggat aaatacgctg gcaaccatca tgccaactgg cgagagttgc ccaatcacct
    44521 gcgaagcggt cggactcagg tattgcaagg atgagctgaa caggatgaag ttcccaaaca
    44581 gcccggcggt cgccactgcc aacaaaatca accagcgtgg cttacgaaac acgcgtaatg
    44641 gcggcaacct cttcttcacc gcaagaatgg cccccaggcc aatactcgcc atcaagaaac
    44701 ggtaaaacac gattgtcgga ggttccatca cctccagcac ctgcttcatt gcaattggca
    44761 acgcccccca gcaaattgct gtggtgagcg ccaaaagaat gccaatgcct gcctgctgct
    44821 tcataccgta ttccctgcaa agcgcatttg cggattaacg tcgcaccatg cgcattttta
    44881 ccggttatcg aatgtaaaaa gccccgcaac gtgttgcggg gctttcatcc gttaccggga
    44941 cgcgaaaaac ttattcagct tcgatgctga taaatttacg gtttttcggg cctttaactt
    45001 cgaatttcac tttaccgtct gctttagcaa acagagtgtg gtcacgaccg caacctacgt
    45061 tagcgccagc gtggaatttg gtaccacgtt gacgaacgat gatgctaccc gccagaacgg
    45121 attcgccacc gaaacgctta acgcccaggc gtttagcttc tgaatcgcga ccgttacgtg
    45181 tggagccgcc agccttttta tgtgccattt gaaatctctc ctcaggtctt aggcgctgat
    45241 gccagtaatt ttcacatcag tgaaccactg acgatggccc tgctgcttac gatagtgttt
    45301 acgacgacga aacttaacga ttttaacttt ctcgccacga ccgtgagcaa caacttcagc
    45361 tttgattacg ccgccatcaa cgaaaggaac gccgattttg acttcttcac cgtttgcgat
    45421 catcagcact tcagcgaact caacagtttc gccagttgcg atgtccagct tttccaggcg
    45481 aacggtctga ccttcgctta ctcggtgttg tttaccacca ctttggaaaa ccgcgtacat
    45541 ataaaactcc gcttccgcgc acaccttttc aatgattcag agtgcgctat aaatattcac
    45601 aatagggcgc gaatattacg caaaacgcac gcctttgaca agtgctacag tcaatacacg
    45661 aagaaaaaaa acacaacttg tacggtaacg tttatctgtg ccattttttc agtacaatca
    45721 ccctatattc ctaaccataa accctaagtt gcctttgttc acagtaaggt aatcggggcg
    45781 aaaagcccgg cttttgcgat gaatttagaa aaaatcaatg agttaaccgc gcaagatatg
    45841 gcgggtgtta atgcggcaat ccttgagcag cttaattccg acgtccaact gatcaatcag
    45901 ttaggctatt acatcgtcag cggcggcggt aaacgtattc gtccgatgat tgctgtactg
    45961 gctgcacgag ctgttggcta tgagggaaat gcgcatgtca ccattgctgc cctgatcgag
    46021 tttatccaca cggcgactct gctgcacgac gacgttgtgg atgaatcaga tatgcgcagg
    46081 ggtaaagcta ccgccaacgc cgcatttggc aatgccgcca gcgtgctggt aggcgatttt
    46141 atttataccc gcgcgttcca gatgatgacc agcctcggtt cactcaaagt gctggaagtc
    46201 atgtcagaag ccgtaaacgt catcgcagaa ggtgaagttc tgcaactgat gaatgttaac
    46261 gatccggaca tcactgaaga aaactacatg cgcgttatct atagcaaaac cgcgcgtctg
    46321 tttgaggctg ccgcgcagtg ttccggaatt ctggcgggct gtacgccgga ggaggagaaa
    46381 ggcctgcagg attatgggcg ctatctcggc actgctttcc agttgatcga cgatttactc
    46441 gattacaatg ccgatggcga acagttaggt aaaaacgtcg gcgacgatct gaacgaaggt
    46501 aaaccgacgc tgccgctgct gcatgcgatg catcatggca caccagaaca ggcacagatg
    46561 atccgtaccg ccatcgaaca gggtaacggt cgccatcttc tgaaaccggt tctggaagca
    46621 atgaacgctt gtggatctct tgaatggacg cgtcagcgcg ccgaggaaga agcagacaaa
    46681 gccatcgcag cgttacaggt gctcccggac accccttggc gagaagcact catcggcctc
    46741 gcgcacatcg ctgttcaacg cgatcgttaa tcccctcccc tcatcccgcg cagtgcgcgg
    46801 gatgattcca gtaaactcca taagaatacc tattcagcgt aataatgcac tgaaaaacca
    46861 gtgcatacat ctttatatat tctgaatatt cacactcttt ataggaactt tttagagcaa
    46921 tcggccatca ggagtatagt gatgctcgac agaagaagtg ttctgaatga aagcgactaa
    46981 cttaaggagt gaggaaagat ggaaagtaat ttcattgact ggcatcccgc tgacatcatt
    47041 gcgggtttgc gcaagaaggg aacatcaatg gcggcggagt ctcgcagaaa tggtttgagt
    47101 tcctcaacgc tggcgaatgc attatcgcgc ccatggccga aaggagagat gattattgcg
    47161 aaagccctgg gaactgaccc ctgggttatc tggccatcac gctaccatga tccgcagacc
    47221 catgagttta tcgacagaac gcagttgatg cgtagctaca ctaaaccgaa aaaatgagtg
    47281 gtctggcggt agccccgcga acggggctgc cagctctcag acgattattc gcctttcaca
    47341 cgctcaatat ttgcacctaa agcgcgcagt ttgtcttcaa tgcgttcgta tccacgatcg
    47401 atgtgataaa tacgatcaac caccgtcgtc ccttccgcaa tacagccagc cagcaccagg
    47461 cttgctgatg cacgcagatc ggttgccata acctgtgcgc cagaaagttt ttcaacaccg
    47521 tgacaaataa cggtattgct ttcgatttcg gcgtgcgcgc ccatacggct cagctctggc
    47581 acatgcataa agcggttttc aaagaccgtt tcggtgatga atccagtccc ttctgccacc
    47641 aggttcaaca gcgtgaactg ggcctgcata tcggtcggga atgccggatg cggcgcggta
    47701 cgtacgttaa cagccttcgg acgtttgcca tgcatatcca ggctaatcca gtcttcgccg
    47761 acctcgatgt ccgctccagc gtcacgcagt ttcgccagca cggcgtcgag agtatctggc
    47821 tgcgtgttac ggcagataat tttgccgcga gaaatcgccg ccgccaccag gaaagtaccg
    47881 gtttcgatac gatccggtag cacgcgatag acaccgccgc ctaaacgttc cacaccttcg
    47941 atgacgatac gatcggtgcc ctgaccgcta attttcgcac ccagcgtaat caggaagttc
    48001 gcggtatcga cgatttccgg ttcacgcgct gcgttttcaa taatcgtggt gccttccgcc
    48061 agggttgcag cacacatgat ggtcaccgtt gcgccaacgc tgactttatc catcacgata
    48121 tgtgcgcctt tcagacgacc atcgacggaa gctttaacgt aaccttcttc cagtttgatg
    48181 gtcgcgccta attgttcgag gccagaaatg tgtagatcaa ccggacgcgc accgatcgta
    48241 caaccgccag gtagtgaaac ttgcccctga ccaaagcgcg ctaccagcgg ccccagtgcc
    48301 cagatagaag cacgcatggt tttaaccaga tcgtaaggtg cgcagaatac attaacgtcg
    48361 cgggcatcaa tatgcacaga accattacgt tctactttcg cacccagctg gcttagcagc
    48421 ttcattgatg tatcgacgtc tttcagtttc gggacgttct ggatctctac cggttcttcc
    48481 gccagtagag cggcaaaaag gataggcaga gcagcatttt tagcgccgga aattgtgact
    48541 tcgccctgga gcttcgttgg cccctgaaca cgaaatttat ccatttagtt tgttctcagt
    48601 taacaattca tatccgctac cggcgaatcg cccatagctc aaaagccgtt cagtttgcga
    48661 tcgcgcgccc actccgcagg ggtatacgct ttgatcgaca cagcatgaat gcggttatcc
    48721 gcaatatatt ccatcagcgg accatagacc gtctgctgtt ttttaacccg actcatgccg
    48781 tcaaacaact cacccacggc aataacctga aagtggctgc catcgccgga aacgtggact
    48841 tcctggaggg agagagcgtt catcaacacg ctctgaattt cattattttc catgggatct
    48901 tcaatcatca gttaataaac cagcgaaaca tcttagagca aagttgcgct ggcataaata
    48961 agcaaaaagc ctcgctgata aatcagacaa ggctcgactt gcaggcaggt ttgccggaca
    49021 ggcggttaac gccatatccg gcctgaaaaa atttaacgag gcagaacatc agcaggcaaa
    49081 ttatacaatt tcgccagggt atacactttg tcgtttaccc cctgaagcgt cacgttgttg
    49141 ccctgctttt tcgccagatc gataagatgg agcagcagtg ccagtccacc cgtatccacg
    49201 cgggacacac ggctaagatc gatgcaggta atccccttca ccgcttcctc acgcatttcc
    49261 caaagcggta gcagaacgtc ctgatccagc tctccggata acgccagtgt gtcacccgtc
    49321 tgcatccagc tcagtgactc gctcattatt ttttctcttc cagagtgatt ttctgttgag
    49381 aaatcgattt cagttgcgca gtcaggccgt cgataccttt ggtacgtagc agcgttcccc
    49441 actcgttttg tttggtggtg atcatactga cgccttcagc aatcatgtcg taagcctgcc
    49501 agttgcccgt ctgggagttt ttacgccact ggaagtccag acgcaccggc gggcggccat
    49561 tcgggtcaat aatggtaacg cgaataggca caatggtttt atcacccagc ggctgttctg
    49621 gcgcaatctg ataggtttga ccgtgataca tcgccagcgc ctgaccgtaa gcctgcttca
    49681 ggtactcacg gaaagcggca aagtaagcat cacgttgagc tggggtcgca ctcttgtaat
    49741 actggcccag caccagcgca ccggcgtatt tcacctgtac gtatggcagc agttcctgat
    49801 caacaatggt acgcagataa tccgggttgg cccgaatttg cggttgctca ttcttcaggc
    49861 gatcgaacgt tttctgcgcc gcctcgtcca tcagcttata cggattggtc tggtctgccg
    49921 cggttgccgc actcagcggt gcaatcacca gcaaagcgac catcattaaa cgtttaaaca
    49981 tgcgtcggtt ctcctgaaat tatttcgttg tacccacagg ttcagtggtt tcattattac
    50041 ctggcgcagc agctggcgca tcgccactat tcttattgtc atcgccttta ctaccgtaaa
    50101 ggaactgacc aatgagatct tccagcacca tcgcagactt ggtgtcctga attgtatcgc
    50161 catccttcag gatagcagtc cccagttccg ggtcttcaaa accgacgttt aatgccagat
    50221 attgttcccc cagcaggccg gaagtacgaa tgctcagcga actggtatca ggaatatggt
    50281 tataacgttg ttcaatttcc agcgttacgc gcggcagata ggttttcggg tccagcgtaa
    50341 tatccgccac ccggcccaca acaacgccac caatactgac cggagagcgc gctttcaggc
    50401 cgccaatgtt atcgaacgtc gcataaagcg tgtaggtcgg ttcagtacgt attgacgtca
    50461 cgttcgccgc cttcaggcaa acaaacagcg ccgccagcag tgctgctaat aaaaagatac
    50521 ccacccaaat ttcatttttt ttcgtttgca tgaactcaat tcccaaacat caatgcggtc
    50581 agcacaaaat ccagccccag aacagccaga gacgagtgga caacggtgcg agtggttgcc
    50641 cggctaatcc cggcagacgt cgggatggcg tcgtagccgt taaacaacga aatccacgtc
    50701 accgtgatgg cgaacaccac gctcttaatc agacagttga ccagatccat acgccagtcg
    50761 acggcatttt gcattgccga ccagaagaac ccgctatcaa tgcctttcca actgacgccg
    50821 accagcgatc cgccccagat ccccacggcg acgaagataa ccgtcaacag tggtaatgaa
    50881 ataaccccag cccagaaacg gggagaaata acccgacgca gcggatccac cgccatcatc
    50941 tccatactgg agagttgctc tgtagcgcgc atcaggccga tttctgcggt tagcgccgaa
    51001 ccagcacgcc cggcaaacaa caacgcggca accaccggcc ccagttcacg cagtagcgat
    51061 aacgccacca gcatacccag actggtttcc gcactataag tggtcagaac cagataacct
    51121 tgcagcccca acaccattcc gatgaacacg ccagaaacta caataatcag catcgacagg
    51181 acgccgacat tatagagctg gcgcaccagc agcggcgcat gtttgcgaaa ttccggtttg
    51241 ccgaccagcg cattgaataa cattaacccg gcccgcccaa acgttctcag ggttttaatc
    51301 cctttatgtc cgagcgacgc cagcgcattt aacagcatga gtggcttaac tccctggtaa
    51361 aagatcagcg tgataatcgc cggcaggata gcggaacgga accggcccgt cagctatccc
    51421 gtccagaaac tgacgtacgc gcggatcagg attcgcctgc aacgcctggg cactgccatg
    51481 agcgacaatt tttttgtccg ccaggatcca ggcgtgatcc gcaatactta acacttccgg
    51541 cacatcgtga gaaaccacca cacaagtcac gcccagcgcg ctgttcagct cagaaatcag
    51601 cttcaccagt acgcccatgg tgatgggatc ttgcccaaca aaaggttcat caaacatgat
    51661 gagatccggc tccagcgcaa tcgcacgcgc cagcgctgca cgccgcgcca tcccaccgga
    51721 aagttcagaa ggcattagtt tagccgctcc acgcagcccc acggcctcca gcttcatcat
    51781 caccgtacta tgcaacaatg gcgcgggaag ttgggtatgt tcgcgcagtg gataggcgac
    51841 gttgtcaaat acgttcatat cagtgaacaa cgccccggac tgaaataaca tgctcatccg
    51901 tttgcgcact gtatacaggc gcgaacgaga catcgccgga atattctcac catcgaaaag
    51961 gatctcacca tgatctggtg cgatttgccc gccaatcaga cggagtagcg tcgttttacc
    52021 gatgcccgat ggccccatga tcgccgtgat cttccctcgc ggcacggtca gggaaatatt
    52081 atcgaagatg cagcgattgc cacgcgtaaa actgacatcg cgcatatcga ctaaattcgc
    52141 cacagactgc tccataattc acccttcgtc ttgcgttgat tttctaagca tggcgctcaa
    52201 tttaaccttg aacccaacat atttacagaa tattacccgc cgtggttagc gaaagctggc
    52261 atttgtttta ctttttagcc gcataaagtc aaaattaaga atccgttacg gctttctgaa
    52321 aatcttcagc ggaccggcga gtatacctga agaaaggacg ttagatgctt ttagctacgg
    52381 cactgttaat tgttggttta cttttggtcg tttacagtgc cgaccgcctg gtttttgccg
    52441 cgtctattct ttgccgaacc tttggcatcc cgccgctgat catcggcatg acggtggtca
    52501 gtattggtac atcgttacca gaaatcatcg tctcgcttgc cgcgtctctg cacgaacaac
    52561 gcgatttagc cgttggtaca gccctcggct caaacattat caatatattg ctgatcctcg
    52621 gtctggctgc gctggttcgt ccttttaccg tccattccga tgttctacgc cgtgaattac
    52681 ccttaatgtt gttggtcagc gtggtggccg gttccgtact ctatgacgga caacttagtc
    52741 gcagcgatgg tatctttctc ctctttctgg ctgtgctatg gctgctgttc attgttaaac
    52801 ttgcacgtca ggctgaacgt caggggactg acagcctgac cagagagcag cttgcagagc
    52861 tgccgcgtga cggcggattg cccgtcgcgt ttttatggct cggcattgcg cttatcatca
    52921 tgccagtggc cacgcggatg gtggttgata acgccacggt gctggcgaat tactttgcca
    52981 tcagcgagtt gacgatgggt ctgacggcaa ttgctatcgg aaccagcctg ccggaactgg
    53041 caaccgcaat agcgggcgtt cgcaaaggtg aaaacgacat tgctgtcgga aatatcattg
    53101 gcgcaaacat ttttaatatt gtcatcgtgt tgggtttacc cgcgctgata acgccaggag
    53161 agattgatcc actggcgtac agtcgtgact acagcgtgat gttgctggtg agcattattt
    53221 ttgcgttgct gtgctggcgg cgctccccgc aaccgggccg tggtgtaggg gtattattaa
    53281 ctggcggatt tatcgtatgg ctggcgatgt tgtactggtt atcgccaata ctcgttgaat
    53341 aactggaaac gcattatgtc gcacgtagag ttacaaccgg gttttgactt tcagcaagca
    53401 ggtaaagaag tcctggcgat tgaacgtgaa tgcctggcgg agcttgatca atacatcaat
    53461 cagaatttca cgcttgcctg tgaaaagatg ttctggtgta aagggaaagt tgtcgtcatg
    53521 gggatgggga aatcggggca catcgggcgc aaaatggccg caacgtttgc cagcaccggt
    53581 acaccttcat ttttcgtcca tcctggtgaa gccgcgcatg gtgatttagg catggtcacc
    53641 ccacaggatg tggtgattgc tatctctaac tcaggtgaat ccagcgaaat cactgcctta
    53701 attccagtgc ttaagcgtct tcacataccg ttaatctgca tcaccggtcg cccggagagc
    53761 agcatggcgc gcgccgcaga tgtgcatctg tgtgttaaag tagcgaaaga agcctgtccg
    53821 ttagggctgg caccgaccag cagcaccacc gccacgctgg ttatgggcga tgccctcgct
    53881 gtcgcgctgt taaaagcacg cggctttact gctgaagatt ttgcgctctc acacccaggc
    53941 ggcgcactgg gtcgtaaact tctgctgcgc gtaaacgata ttatgcatac gggcgatgag
    54001 atcccgcatg ttaagaaaac ggccagtctg cgtgacgcat tgctggaagt tacccgcaaa
    54061 aatcttggta tgactgtcat ttgcgatgac aatatgatga ttgaaggcat ctttaccgac
    54121 ggtgatttac gccgtgtctt cgatatgggc gtggatgttc gtcagttaag tattgccgat
    54181 gtgatgacgc cggggggaat acgcgtgcgc cctggcattc tggccgttga ggcactgaac
    54241 ttaatgcagt cccgccatat cacctccgtg atggttgccg atggcgacca tttactcggt
    54301 gtgttacata tgcatgattt actgcgtgca ggcgtagtgt aaagattcaa ggataaacaa
    54361 caatgagcaa agcaggtgcg tcgcttgcga cctgttacgg ccctgtcagc gccgacgtta
    54421 tagcaaaagc agagaacatt cgtctgctaa tcctcgacgt tgatggcgta ctgtcagatg
    54481 gcctgattta tatgggcaat aacggcgaag agctgaaagc gttcaatgtt cgtgacggtt
    54541 atggcattcg ttgtgcgctc acctctgata ttgaagtcgc tatcattacc gggcgaaagg
    54601 ctaaactggt agaagatcgt tgtgccacat tggggatcac tcacttgtat caggggcagt
    54661 caaacaaact gatcgccttt agcgatctgc tggaaaaatt ggcgattgcc ccggaaaatg
    54721 tggcttatgt cggcgatgat ctcatcgatt ggccggtaat ggaaaaagtg ggtttaagcg
    54781 tcgccgtggc cgatgcgcat ccactgttga tcccgcgcgc cgattacgtg acgcgcattg
    54841 ctggcggtcg tggcgcagtg cgagaagttt gcgacttatt actcctggcg cagggcaaac
    54901 tggatgaagc caaagggcaa tcgatatgag taaagccaga cgttgggtta tcattgtgct
    54961 atcactggcg gttctggtga tgatcggtat taatatggcc gaaaaagacg ataccgccca
    55021 ggtggtcgtc aacaacaatg atcccaccta taaaagcgag catacggaca cgctcgtcta
    55081 taacccagaa ggggcgctaa gctatcgatt gattgctcaa cacgttgaat attattccga
    55141 tcaggccgtt tcgtggttta cgcagccggt acttaccacg tttgataagg ataaaatccc
    55201 gacatggtcc gtaaaagcag ataaagccaa gctgaccaat gaccggatgc tctatttata
    55261 tggacacgtt gaagtcaacg cacttgtgcc ggactctcaa cttcgcagaa tcacgacgga
    55321 taacgcgcag atcaatctgg tgacgcagga tgttacctct gaagacctcg tcacgttata
    55381 cggaacaaca tttaactcca gcggtctgaa aatgcgcggc aacttacgca gcaagaacgc
    55441 cgagctgatt gaaaaggtta gaacatccta tgaaattcaa aacaaacaaa ctcagcctta
    55501 atcttgtgct tgccagctca cttctggccg ccagcattcc ggcatttgcc gtaaccggag
    55561 acactgatca gccgatacac attgaatcgg accagcaatc tcttgatatg caaggcaacg
    55621 tggttacctt taccggtaat gtcatcgtca cccagggcac cataaaaatt aatgccgaca
    55681 aagtggtcgt tacccgtccg ggtggcgaac aaggtaaaga agtgattgac ggctacggta
    55741 aaccggcaac gttctaccag atgcaggaca acggtaaacc cgttgaaggt cacgcttccc
    55801 agatgcacta cgaactggca aaagattttg tcgttctgac gggtaatgct tatctgcagc
    55861 aggtcgatag caacattaag ggcgataaga tcacttacct ggtgaaagag cagaaaatgc
    55921 aggctttcag cgacaaaggc aagcgtgtaa ccaccgttct ggtgccgtcg cagctgcagg
    55981 acaaaaacaa caaaggccag accccggcac agaagaaggg taattaattc gttatggcaa
    56041 cattaactgc aaagaacctt gcaaaagcct ataaaggccg tcgcgtggta gaagacgtca
    56101 gcctgaccgt caactccggg gaaattgtcg gtctgctggg gccaaacggt gccggtaaga
    56161 ccaccacttt ctacatggtt gtaggcattg tgccgcgcga tgcgggcaac atcattattg
    56221 atgatgacga tatcagtctg ctacctctgc atgcacgcgc gcgccgcggt atcggctatc
    56281 tgccacagga agcctccatt ttccgtcgcc tcagcgttta cgataacctg atggcggtac
    56341 tgcaaattcg tgacgacttg tctgctgaac agcgtgaaga ccgcgcgaac gagctgatgg
    56401 aagagtttca cattgagcac ctgcgtgaca gcatggggca gtcactctcc gggggtgaac
    56461 gtcgccgtgt agaaattgcc cgcgcactgg ctgcgaatcc gaaatttatt ctgctcgacg
    56521 aaccgtttgc cggggttgac ccgatctcag ttatcgacat taaacgcatc attgagcacc
    56581 tgcgcgacag cggcctgggc gtgctgatca ctgaccacaa cgtgcgtgaa acactggcgg
    56641 tttgtgaacg cgcttatatc gtcagtcagg ggcatttgat cgcccacggc acgcctacag
    56701 aaatcttaca agacgaacac gttaagcgtg tataccttgg ggaagacttc agactctgat
    56761 agggtagaag tttgcgacgt tttagcagga gagtacgatt ctgaacatga agcaaggttt
    56821 gcaactcagg cttagccaac aactggcgat gacgccacag ctccaacagg caattcgtct
    56881 gttgcagttg tcgacgctgg aacttcagca ggagctacag caggcgctgg agagtaatcc
    56941 gctgcttgag caaatcgaca ctcatgaaga aatcgacacc cgcgaaacgc aagacagtga
    57001 aacgctggac accgccgacg cgctcgaaca aaaagagatg ccggaagagc tgccgctcga
    57061 tgccagttgg gacaccattt acaccgctgg tacaccatcc ggcaccagcg gtgactacat
    57121 tgacgacgag ctgccggtct atcagggcga aacgacgcag accttgcagg attacctgat
    57181 gtggcaggtc gagctgacac cgttttccga cactgaccgc gctattgcta cctctatcgt
    57241 cgatgccgtt gatgacaccg gttatctgac tgtcccgctg gaagatattc tcgaaagtat
    57301 gggcgatgaa gagatcgaca tcgacgaagt tgaagccgtc cttaagcgga tccaacggtt
    57361 tgatccggtc ggtgtggcgg caaaagatct gcgtgactgc ctgctgatcc aactctccca
    57421 attcgataaa accacgccgt ggctggaaga ggccagactg atcattagcg atcatctcga
    57481 tctgttagcc aatcacgact tccgcacttt aatgcgcgtc acgcgtctga aagaagatgt
    57541 gctgaaagaa gccgtcaatc tgatccagtc gctcgatccg cgccccgggc aatcgatcca
    57601 gactggcgaa cctgagtatg tcattccaga tgtgctggtg cgtaagcata acggtcactg
    57661 gacggtagaa ctcaacagtg acagcattcc tcgtctgcaa atcaaccagc actacgcctc
    57721 gatgtgcaat aacgcgcgca atgatggtga cagccagttt atccgcagca atctgcaaga
    57781 tgccaaatgg ttgatcaaga gtctggaaag ccgtaacgat acgctactgc gcgtgagtcg
    57841 ctgtatcgtt gaacagcagc aagccttctt tgagcaaggc gaagaatata tgaaaccgat
    57901 ggtactggcc gatatcgccc aggctgtcga aatgcatgaa tcgacgatat ctcgcgtgac
    57961 cacgcaaaaa tacctgcata gtccacgagg catttttgaa ctgaagtatt tcttttccag
    58021 tcacgtcaat accgagggcg gcggcgaagc ttcctccacg gcgattcgtg cgctggtgaa
    58081 gaaattaatc gcggcggaaa acccagcgaa accgttgagc gacagcaagt taacctcttt
    58141 gctgtcggaa caaggtatca tggtggcacg ccgcactgtt gcgaagtacc gagagtcttt
    58201 atccattccg ccgtcaaacc agcgtaaaca gctcgtttga cccaaccgat aaggaagaca
    58261 ctatgcagct caacattacc ggaaataacg tcgagatcac cgaggcactg cgcgaatttg
    58321 ttacagccaa atttgccaaa cttgagcaat attttgaccg aatcaaccag gtctatgttg
    58381 ttctgaaagt ggagaaagtc acccacacct cagatgcaac actgcatgta aacggtggtg
    58441 aaattcatgc cagcgcggaa ggtcaggaca tgtacgctgc cattgatggc ttaattgata
    58501 agctggcacg ccagttgacc aaacataaag ataaactgaa acaacactaa ttgtccgggc
    58561 aattagcatg tgcatggcgg cctgttgtgc ggcacaacgg gccatttgta cggttaatgc
    58621 tccgagcctg ttccactgtt tgagtgggca ggttcttagg tgaaattatg acaaataatg
    58681 atacaactct acagcttagc agtgttctta acagggaatg tacgcgaagc cgcgtccact
    58741 gtcagagcaa aaaacgcgcc ctggaaatca tcagcgagct ggcggcgaaa caacttagcc
    58801 taccgcctca ggtggttttt gaagctatcc tgacgcggga aaaaatgggc agtaccggta
    58861 tcggcaatgg tattgccatt ccgcatggca aactggaaga agatactctg cgtgccgttg
    58921 gcgttttcgt tcagctcgaa acgcctatag ctttcgatgc catcgacaac caaccggtgg
    58981 acttactttt tgccctgctg gtgccggcag accaaactaa aacgcacctg catactctgt
    59041 cgctggtggc gaaacgtctg gcggacaaaa ccatctgccg ccgtttacgc gcagcccaga
    59101 gcgatgaaga gctgtatcaa atcattacgg ataccaaagg tactccggat gaagcgtagt
    59161 tattcggtaa tgtctctttt agacgttgtg aggagaaaca gtacatggta ctgatgatcg
    59221 tcagcggacg ttcaggttca ggtaaatctg tcgccctgcg tgcgctggaa gatatgggtt
    59281 tttactgcgt ggataacctt cccgtagtgt tgttacccga tctggctcga accctggccg
    59341 atcgcgagat ttctgccgcc gtcagcattg atgttcgtaa tatgccggag tcaccagaaa
    59401 tattcgaaca ggcgatgagt aacctgcctg acgctttctc accgcaacta ctgttcctgg
    59461 atgccgaccg taatacctta attcgtcgtt acagtgacac gcgccgactg catccgcttt
    59521 ccagcaaaaa cctgtcgctg gaaagtgcta tcgacaaaga aagcgatctg ctggagcctc
    59581 tgcgttcgcg agcggatctg attgtcgata cctcagaaat gtccgttcac gagctggcag
    59641 aaatgctgcg tacccgtctg ctgggtaaac gtgaacgcga actgacaatg gtctttgagt
    59701 ctttcggctt caaacacggt atccctatcg atgcagatta cgtctttgac gtgcgcttct
    59761 tgccgaaccc gcactgggat ccgaaactgc gtccaatgac aggtcttgat aagcctgtcg
    59821 ccgcgttcct cgaccgccac acagaagtac acaattttat ctaccagacg cgaagctatc
    59881 ttgagctatg gttacctatg ctggaaacca acaaccgtag ctacttgacg gtcgccattg
    59941 gttgtaccgg cgggaagcac cgttcggtgt atattgcaga gcaactggca gactacttcc
    60001 gctcgcgcgg taaaaacgtc cagtcacgcc atcgtacgct ggaaaaacgt aaaccatgac
    60061 tgtcaagcaa actgttgaaa tcacaaacaa gctgggcatg catgcccggc ctgcaatgaa
    60121 gctgtttgaa ttaatgcagg gttttgacgc tgaagtgctg ttacgtaatg acgaaggcac
    60181 cgaggctgaa gccaacagcg ttattgcgct gctgatgttg gattctgcca aaggacggca
    60241 gattgaagtt gaagcgaccg gtccacagga agaggaagca ctggccgccg ttatcgccct
    60301 ctttaattct ggttttgatg aagattaatc ttcatcactt tgacatacaa ctcccttcaa
    60361 actccccccg acaataagaa aatcacgtac tgaaatcgtt ctcaatcaac gtcaattgta
    60421 catttcgtgc gctttttact ttcagaagaa ccttaagaaa accttaagaa gcattgttta
    60481 ggtttcgttt aagttaatcg accatactgg agatcgtcag aaaatatttc caggagatgg
    60541 catgattcgc ttatctgaac aaagtcccct gggcaccgga agacatcgga aatgttatgc
    60601 gcatccggaa gatgcccaac gctgtatcaa gattgtctac catcgtggcg atggtggcga
    60661 taaagagatc cgtcgtgagt taaagtacta cgcgcatctt ggtcgccggt taaaagactg
    60721 gagtggaata ccgcgctatc acggtaccgt cgagacggat tgcggaaccg gttatgttta
    60781 tgatgtgatt gctgattttg acggcaagcc ctccattacg ctgaccgaat ttgctgaaca
    60841 atgccgttac gaagaagacg ttgcacaact gcgccaatta ctgaaacagc tgaagcgtta
    60901 tttgcaggat aaccgtatcg tgacgatgtc gctgaagccg cagaatatcc tctgtcaccg
    60961 cattagtgag tcagaggtta tccccgtcgt ctgcgacaac attggtgaaa gcacattgat
    61021 tccactggcg acctggtcaa agtggtgttg tctgcgtaag caagagcggt tgtggaaacg
    61081 atttatcgcc caaccggcgc tggctattgc cctgcagaaa gatctgcaac ctcgtgagag
    61141 caaaacgctg gccctcgctt cgcgcgaagc ttaatccagc tggtgttgtt gcataaacgg
    61201 ctcaccgcct aactgataca tctgccgtaa aatccacgcc tgacggctgc gcacgtagcc
    61261 cgatggcgcg gagactttga aacgaagtgg attaggtaat acagctgcca gtaaggcagc
    61321 ttccgatcgg gtaagtttgc tcgcgggttt gtggaaataa cgttgtgccg cagcttcgac
    61381 gccaaacaca ccgtcgccaa attcggcgat attcaggtaa accgtcagga tacgcttttt
    61441 gctccagaca gtttctatcc ccagcgttaa tccggcttcc agcccttttc gaacccagct
    61501 acgcccatcc cataaaaaga gatttttggc tgtctgttga gaaatcgttg aagcaccgcg
    61561 aatacggttt tcattgcgct cattgtgcgc cagggctttc tcaatggaag cgacatcaaa
    61621 gccccagtgc tcaggaaatg tctgatcttc tgcggcaatc accgccagtc ccatccacgg
    61681 cgagatttga tccatactga ccctgtcaga atgtgccacg taacgaaaat taccatgcag
    61741 ccaggcgctg acctgtcgct cgaccattac cgcagagaag ggaacaggtg caacgctaaa
    61801 caacgcgatg cccccgcccc agaaaacggc gaggacaacc attaaccgca gaagaaaacg
    61861 gcgaacgaaa ctaaacaccg ttaagcggct tttactcatt cagccagaac cagcacgcgg
    61921 gaaaccagct tatcaatgcc gctcgccgct tctgcaatgt tctgcgccag catatatgcc
    61981 ggggtggtga caatcttatt gtcttcatca accacgatat catcgacagg acacggcaca
    62041 tgctccgcgc ccatctcttc cagcacttct gcggtatcga tatcagtacc gatggtcaaa
    62101 cgcagcggga aatcgaaaat tttcggcagc atcgccgggg caatacacat aaaaccaagc
    62161 ggttttccgg cctgatgcat cgcttgtgcc agcgccttta attcacggtc aacggtgcat
    62221 tcgcgaccaa gactggcaaa attgcttaaa ttctttgccg cgccaaaccc ccccggcaca
    62281 atcaacgcat ccagttcagc ggcatcggcc tgggccagag gacggatttc accgcgcgtt
    62341 attcgtgccg cttcaatcag cacattgcgc gtttccgtca tcgcttcgcc agttaaatga
    62401 ttgataacat caacctgctg cttatccggt gcaaagcaga ccgcctgcgc accgctgcgt
    62461 gaaatagcta acaacgtcaa caccgcttca tgaatttcag aaccgtcata gacgccgcat
    62521 ccgctcagaa ttacgccaat tttcttcatt atgatcatcc tttcgcaatt gactgaaaca
    62581 cattaatttt tttaataaaa atggtacgca tcacacattt aactgattca tgtaacaaat
    62641 catttaagtt ttgctatctt aactgcgtgc ggcctgaaaa acagtgcagt gcccttgtaa
    62701 ctcatcataa taatttacgg cgcagccaag atttccctgg tgttggcgca gtattcgcgc
    62761 accccggtct agccggggtc attttttagt ggcttttgcc acccacgctt tcagcacttc
    62821 tacgtcgtga cgccactctt ctttcatctc ttcaatccat tcaccgacgt tatcttccca
    62881 ggcgggaagg tcaggagact gaatttgctg acccagctgt tgcagatggc gcaaccccac
    62941 tgaccccgcc gcacctttaa ttttatgtcc ttcctcaaca atgccttttt tatcctgcgc
    63001 cgtcagattc gactccagca cgttgacata gcctggcatc atcttctcaa acaccgctaa
    63061 cccgtcggtg atcagcttcg gtcctacaag ttcgagatac tgttccagca tgggaatatc
    63121 gagcaatgct tctgatttac tgttctcttc tgtcgtcacc gtactctcct catcatcctg
    63181 ggtatcccag aatttcttga tcatcgcggt tagcgccgga acagaaagcg gcttactcag
    63241 cacatcatcc attccagcat tgaggtactc ttgtttgtct ttcagcacgt tagcggttaa
    63301 ggccaccagc ggcggtaaat cctctcgcgg ataacgcttc gtcagttcac gagagatatc
    63361 cagcccggtc atatctggca actgaatatc cagcaacacc aggtcgtatt cgcccggttt
    63421 aaacatctcc agcgccgcct tgccggtcat ggcgacatca acgctgttac ctaatttttc
    63481 cagcacagaa cgcgcgacaa tcacgttcag ttcaatgtct tccaccagca gcacattcag
    63541 cgccggtaaa ggcatatcgt cttcatcaaa cgcatcatcg acctcttctg ctaccgacgg
    63601 tgcgtggatc gtcaacgtaa aggttgaacc tttaccctgt tcgctggtaa ccgtaatatc
    63661 gccgcccata tttttcgcca gacgacgaga aacggccaga ccaataccgg tgccggtggc
    63721 aggtttaccg ccatgactgt ctttcacctg gtaatacatg gcgaagattt tatccagctc
    63781 atcctgcgga atgccgatac cagaatcttc cacttcaaaa tgcagcatat cgccttcatc
    63841 gtagcgcacg cgcacggtaa cctggccttg ctgggtgaat ttgacggcgt tactgatgag
    63901 gttccacagg atctgccgta aacgcgtccc gtcggtaatg acctgatgcg gtaatggcag
    63961 cgtcggctcc aggttaaagc gcaatccttt ttgttgcgcc tgcaatgcgg agagattttc
    64021 cagatcggca aggaagctgg tgaaatcaac cggttgatta tcaagctgga ccttgcgccg
    64081 ttccatctta tccatgtcga taatatcgtt aaagatattc cccagcgtga cggccgaaac
    64141 atggatggtc ttgagatatt tttcctgctc ggcggtgagt tcggtatcca gcagaatgcg
    64201 gctcaggccg acgataccgt tcagcggtgt acgcaattcg tggctgatgg tggaaataaa
    64261 cgtcgttttg tcgcggctgg cgcgttcaag cgcatcctga taccgcttac gctcggtaat
    64321 gtcgcgacca aagcccatca aaccgtgacg tttacccacg cggtcgtagt acggcacttt
    64381 acgaatttca aagcaggctt tgcgtccgtc gggataatcc agccactgct cataggtcag
    64441 tgacacatta tgacggaaca ctttttcatc ggtttcaatg actttcgcag cggcttccgg
    64501 tgagtaaaca tcagcaggtt tcaggtgaac cagttgtttt tcgctttttc cggtcagcag
    64561 ctccatcgcg cggttacagc cggaaaactc tttatcttcg ttacgataaa aaaccaggtc
    64621 gggtgaagca tcaaggaagg aacgtaaaaa tgaggattgc tgctcgagct gaatttgtgt
    64681 ctcttcgcgc tctttgattt caattttcag ttggccgaag gtttcctgca gttctgcttc
    64741 cgctttttca cgaacggcaa tttcctgatt tagctgggca atattatctt ttaactgaac
    64801 gttgaggctc aaatcgcgct cgcgcatctc ctccagtttt tgcaccagcc gtgacagacg
    64861 ttgtcgtgac tcctccagtt gctcgacgac caccgataga aagtagaccg cccacggcgt
    64921 aatcagcaaa ccaaagaaga tagaacgaat aacatcaatg ctttcgacct gaccatgcag
    64981 caccatggtt accgccattt gtaccacaat ggcaagaacg acgagggcca gcgccagcaa
    65041 cattgagaag cgcaccagac ctaacttcat catcaggtca acataatact gcgccagcag
    65101 acgaatttgc ttcattaggg aattccttca cgacaacctg ccccaataat acccaattct
    65161 gacagttacg ttataaattg tgtgagaaat gcggaattcc cctcaacgac ctactccgtt
    65221 ggaggtagcc acggacgtcc cagcgccgat ccctgtacgc catgttcatt cagatagcga
    65281 tccagctcga ccattcccgt ccatcgattt tcgcaccaca gaggagcaag cagcgtcggg
    65341 cgacgggcgc tggcggaaat gcggtggtag atcacttccg gtggcgtatg gcgaatcatt
    65401 tctccggcag tgagcgtgta atcctccagt tcaataccgt tcaaacgccc cgcttcccag
    65461 gctttcgcca taatgctgcc tttcacaata tgcagcggat gcagctttat gccgtccacg
    65521 ccggtttcaa ccactcgctc cagcgtttgc aaacattcag cctgcccttc gccaggcagg
    65581 ccgacaatta agtgagaaca caccttcagc ccgcgctgac gtgccagctg ggttgtacgc
    65641 tgataacagg caaaatcatg gccgcgatta atgcgatgca gggttttgtc gtgggcggtt
    65701 tgtagcccca gctccagcca cacttcgtag ccctggtcct tatattcgca aagcagatcc
    65761 agcaccgcat ccggcacgca gtccgggcga gtaccaacac acaaaccgac aatattggcc
    65821 tggctcaccg cctgctgata catagaacgc agcacctgaa cttccgcaaa ggtgctggta
    65881 tacgcctgaa agtaggccag atagcgttta gcgcggttaa ctaaattcgc ctggtgcgcc
    65941 agttgctcgg caatggaacg atgttgctgc gcttcatcgg caaatgaggc aacattacag
    66001 aatgtgcagc cgccacgccc gatggtaccg tcacggttag ggcagctaaa accgccgtgc
    66061 agcgtcagct tatgcacctt ttgcccataa cgacgggtga gatcaccacc aaacatattg
    66121 actaatttct gtaactgcat aatctgatag accgcgcctt gaaaagaggc caaagcctgc
    66181 catttttagc tcaattcggc gatgacttgg atcaatcgtc ccggcctgct tttatcaact
    66241 gcataaccaa tcaaaattac cgaaatttca tgcataatca cataaatcac ttttgcttat
    66301 cttgtgtcag atttttttat ctccttatgg attttaggca aaacagtagc atgaaacgtc
    66361 attaccaatt aaggcagtat aaaatgctgg ttttctcgtc agttcaaggc aggataaggg
    66421 ttaacacgcc tttatgacag tcaggaattg gctgtttctc taacgacgcc ccttttagcc
    66481 ttaattagaa aatccatttt aatttcagtc aattaataaa gaattttgcg ctaaagcaca
    66541 tttctgtacc aataagcttg ccatttgacc tgtatcagct ttcccgataa gttggaaatc
    66601 cgctggaagc tttctggatg agcagcctgc tcatcatatt tatgcagtaa ttgagatccc
    66661 ctcttcaccg tattaaccga tgcgaaaagg acaacaaggg ggcgaatgcg aggcgcgcgt
    66721 atgacacgca aaccccgtcg ccacgctctt tctgtgcccg tgcgcagcgg ttcggaagtg
    66781 gggttcccgc agagcctggg ggaggttcac gatatgttgt acgataaatc ccttgagagg
    66841 gataactgtg gtttcggcct gatcgcccac atagaaggcg aacctagcca caaggtagtg
    66901 cgtactgcaa tacacgcact ggcccgcatg cagcaccgtg gcgcgattct cgccgatggt
    66961 aaaaccggtg acggttgcgg cttgctgtta caaaaaccgg atcgcttttt tcgcatcgtt
    67021 gcgcaggagc gcggctggcg tttagcgaaa aactacgccg tcgggatgct cttcctgaat
    67081 aaagatcctg aactcgccgc tgccgcacgc cgcatcgttg aagaagagct gcaacgcgaa
    67141 accttgtcga ttgtgggctg gcgtgatgtc cccactaacg aaggcgtgct gggtgaaatc
    67201 gccctctcct ctctgccacg cattgagcaa atttttgtga acgccccggc aggctggcgt
    67261 ccgcgcgata tggagcgccg tctgtttatc gcccgccgcc gcattgaaaa gcgtctcgaa
    67321 gccgacaaag acttctacgt ctgtagcctg tcgaatctgg tgaacatcta taaaggtctg
    67381 tgtatgccgg cggatctgcc gcgcttttat ctagatcttg cggacctgcg tctggaatcg
    67441 gccatttgcc tgttccacca gcgcttctcc actaacaccg taccgcgctg gccgctggcg
    67501 caaccgttcc gctacctggc gcataacggt gaaatcaaca ccatcaccgg taaccgccaa
    67561 tgggcgcgtg cgcgtactta taaattccag acaccgctta tccctgacct gcacgacgcc
    67621 gcaccgttcg tcaacgaaat cggctctgac tccagttcga tggataacat gctggaactg
    67681 ctgctggcag gcgggatgga tatcatccgc gccatgcgtc tattagtacc acccgcctgg
    67741 cagaacaacc cggatatgga cccggaactg cgtgccttct ttgactttaa ctccatgcat
    67801 atggagccgt gggatggccc ggcgggcatc gtgatgtctg acggtcgttt tgctgcctgt
    67861 aacctcgacc gtaacggtct gcgtccggcg cgctacgtca tcaccaaaga taagctcatc
    67921 acctgcgcct ctgaagtcgg tatctgggat taccagcctg acgaagtggt cgaaaaaggc
    67981 cgcgtcgggc caggcgaact gatggtaatc gacactcgca gtgggcgtat tctgcactcg
    68041 gcagaaaccg atgacgatct gaaaagccgc cacccttata aagagtggat ggagaaaaac
    68101 gtccgccgac tggtaccgtt tgaagatctg cccgatgaag aagtgggtag ccgcgaactg
    68161 gacgacgaca cgcttgccag ctaccagaaa cagtttaact acagcgcgga agagctggac
    68221 tccgtaattc gcgtactggg cgaaaacggt caggaagcgg tcggttcgat gggcgatgat
    68281 accccattcg ccgtgctctc cagtcagccg cgcattattt acgactactt ccgccagcag
    68341 tttgcccagg tgactaaccc gccaatcgac ccgctgcgtg aagcgcatgt tatgtcgctc
    68401 gccaccagta tcggtcgtga aatgaacgtc ttttgcgaag cagagggcca ggcgcaccgt
    68461 ttaagcttta aatcgccgat tctgctctac tccgatttca aacagctcac gacgatgaaa
    68521 gaggagcact accgcgcaga tacgctggat atcacctttg acgtcaccaa aaccacgctc
    68581 gaagcgacgg tcaaagagct gtgcgacaaa gccgaaaaaa tggtacgtag cggcaccgtg
    68641 ctgctggtgc tctccgaccg gaatatcgct aaagatcgcc tgccggttcc ggctccaatg
    68701 gcggttggcg cgatccagac ccgtctggtc gatcaaagcc tgcgttgcga tgccaacatc
    68761 atcgtcgaaa ccgccagcgc ccgcgatccg caccacttcg ccgtgttgct gggtttcggc
    68821 gcgacggcta tttatccgta ccttgcctat gaaacgctgg gccgcctggt agacacccac
    68881 gcgattgcca aagattatcg taccgtgatg ctcaactacc gtaacggcat caacaaaggc
    68941 ttgtacaaaa tcatgtccaa aatgggcatc tccaccatcg cctcttaccg ctgctcgaaa
    69001 ctgtttgaag cggtcggtct gcacaatgat gtagtgggcc tgtgcttcca gggggcggtc
    69061 agccgcattg ggggggcgag ctttgaagac ttccagcagg atctgctgaa tctctcgaaa
    69121 cgagcctggc tggcgcgcaa gcccatcagc cagggcggcc tgctgaaata cgtccacggc
    69181 ggcgaatacc acgcctacaa cccggacgtg gtgcgcacgc tgcaacaggc ggtacagagc
    69241 ggcgagtata gcgactatca ggaatacgcg aagctggtta atgagcgtcc ggcaaccacg
    69301 ctgcgcgatc tgctggcaat tacgccgggt gaaaacgcgg tcaacattgc cgatgttgaa
    69361 ccggcaagcg aactgtttaa acgcttcgat accgcggcga tgtctatcgg cgcgttaagc
    69421 ccggaagccc acgaggcgct ggcggaagcg atgaacagca tcggcggaaa ctcgaactcc
    69481 ggtgaaggtg gtgaagaccc ggcgcgctac ggcaccaaca aagtgtcgcg catcaagcag
    69541 gtggcttccg gtcgctttgg ggttactccg gcgtatctgg tcaatgccga cgtcattcag
    69601 attaaagtcg cccagggcgc gaagccgggc gaaggcggtc agttgccggg cgacaaagtc
    69661 actccttaca ttgccaaact gcgctattcg gtgccgggcg tgacgctgat ctccccgccg
    69721 ccgcaccacg atatctactc tatcgaagac ttagcgcagc tcattttcga cctcaagcag
    69781 gttaacccga aagcgatgat ctccgtgaag ctggtttccg aaccgggcgt tggcaccatc
    69841 gccaccggtg tggcgaaagc ttatgccgat ttaatcacca tcgcaggcta tgacggcggc
    69901 accggcgcaa gtccgctttc atcggtgaaa tacgcaggct gtccgtggga gctgggactt
    69961 gttgaaacac aacaggcgct ggttgccaac ggcttgcgtc ataaaatccg tttgcaggtc
    70021 gatggcggcc tgaaaacggg tgtcgatatc atcaaagcgg cgattctcgg cgcagaaagc
    70081 ttcggcttcg gcactggccc aatggtcgcg ctcggctgta aatatctgcg tatttgccac
    70141 ctgaacaact gcgcaacggg tgtggcaact caggatgaca aactgcgtaa gaatcactat
    70201 cacggcctgc cgttcaaggt gacgaattac tttgagttta tcgcccgtga aacccgcgag
    70261 ctgatggcac agcttggcgt aacacgtctg gtggatctga ttggtcgcac cgacctgctg
    70321 aaagagctgg acggtttcac cgccaaacag cagaaactgg cgctgtcgaa gctgctggag
    70381 accgccgaac cgcatccagg taaggcactc tactgcaccg aaaccaaccc gccgtttgat
    70441 aacggcctgc tgaacgcgca gttgctgcaa caggcgaaac cgtttgtcga tgagcgccag
    70501 agcaaaacct tctggttcga tatccgcaac accgaccgct ctgtcggcgc gtcgctttca
    70561 ggctatatcg cccagacgca cggcgatcag gggctggcag ccgatccgat taaagcgtac
    70621 ttcaacggca ccgcaggcca gagcttcggc gtgtggaacg cgggcggcgt ggaactgtac
    70681 ctgaccggtg atgccaacga ctatgtcggt aaaggcatgg cgggcggctt aatcgccatt
    70741 cgtcctccgg ttggttccgc cttccgcagc catgaagcaa gtattatcgg caacacctgc
    70801 ctgtatggag cgacgggtgg tcgtctgtat gccgcaggcc gcgcgggtga acgtttcggc
    70861 gtgcgtaact ccggtgcaat caccgtggta gaaggcattg gcgacaacgg ctgtgaatat
    70921 atgacgggcg gtatcgtctg tattctgggt aaaaccggcg ttaacttcgg tgcgggtatg
    70981 accggcggct tcgcttacgt tctcgatgaa agcggcgatt tccgcaaacg cgttaacccg
    71041 gaactggtgg aagtattaag cgttgacgat ttggcgatcc atgaagagca tctgcgtggt
    71101 cttatcaccg agcatgtgca gcataccggt tcccagcgtg gtgaagagat tctggcgaac
    71161 tggtcaacct tcgccactaa atttgcgctg gttaaaccga agtccagtga tgtcaaagca
    71221 ctgctgggtc atcgtagtcg tagcgcagca gagttgcgcg tgcaggcgca gtaaggggta
    71281 gcaacaatga gtcagaatgt ttatcaattt atcgacctgc agcgcgttga tccgccaaag
    71341 aaaccgctga agatccgcaa aattgagttt gttgaaattt acgagccgtt ttccgaaggc
    71401 caggccaaag cgcaggctga ccgctgcctg tcgtgcggca acccatactg cgagtggaaa
    71461 tgcccggtac acaactacat cccgaactgg ctgaaactcg ccaacgaggg gcgtattttt
    71521 gaagcggcag aactgtcgca ccagaccaac actctgccgg aagtatgcgg acgtgtctgc
    71581 ccacaagacc gtctgtgcga aggttcctgc accctgaacg atgagtttgg cgcggtgacc
    71641 atcggcaaca ttgagcgtta tatcaatgat aaagcgttcg aaatgggctg gcgtccggat
    71701 atgtccggcg tgaaacagac cggtaaaaaa gtggcgatta tcggcgcagg tccggcaggt
    71761 ctggcgtgtg cagatgtcct gacgcgcaac ggcgtacaag cggtcgtctt cgaccgtcac
    71821 ccggaaattg gcggcttgct gaccttcggt attccggctt tcaaactgga aaaagaggta
    71881 atgacgcgcc gccgtgaaat cttcaccggc atgggtattg aattcaaact caataccgaa
    71941 gtgggccgcg acgtacagct ggacgatctg ctgagtgatt acgatgccgt gttccttggc
    72001 gtcgggactt atcagtcaat gcgcggcggg ctggaaaacg aagacgccga tggcgtgtac
    72061 gcagcgctgc cgttccttat cgccaacacc aaacagttaa tgggctttgg cgaaactcgt
    72121 gaagaaccgt tcgtcagcat ggaaggcaaa cgcgtggtag tacttggcgg tggcgatacc
    72181 gcaatggact gcgtgcgcac gtctgtacgc cagggggcaa agcacgttac ctgtgcctat
    72241 cgtcgtgatg aagagaacat gccgggttcc cgccgcgaag tgaaaaacgc gcgggaagaa
    72301 ggtgtagagt tcaaattcaa tgtccagcca ctgggaattg aagtgaacgg taacggcaaa
    72361 gtcagcggcg taaaaatggt gcgtactgaa atgggcgaac cggatgccaa aggccgtcgc
    72421 cgcgcggaga tcgtggcagg ttccgaacat atcgttccag cagatgcggt gatcatggcg
    72481 tttggtttcc gtccacacag catggaatgg ctggcaaaac acagcgtcga gctggattcg
    72541 caaggccgca tcatcgcccc ggaaggcagc gacaacgctt tccagaccag caacccgaaa
    72601 atctttgctg gcggcgatat cgtccgtggt tccgatctgg tcgttaccgc tattgccgaa
    72661 ggtcgtaagg cggcagacgg cattatgaac tggctggaag tttaagctct gtcccagcgc
    72721 cgcctcctca gaaaagagtc ggcgctgaaa ttctcctttc gttctggaaa gcgcctcgca
    72781 taatctgaag aaaaccaatg taatacgcac atctggattg ttaccggcag atgcccggcg
    72841 tatagctgat tccattattc aacactgcgc catataaagg gaaagataat ggaatcgctc
    72901 tcagagagaa cctcaacagg ctaccagcaa atccacgacg gtattattca cctggtcgat
    72961 agcgcccgga cggaaacggt acgtagcgtt aacgcgctga tgaccgcgac atactgggaa
    73021 attggccgac gaattgtcga atttgaacaa ggtggcgagg ccagggctgc gtatggtgcg
    73081 cagctaatca agcgactatc aaaggattta agtctaaggt ataagcgtgg gttctctgca
    73141 aaaaacttac gccaaatgag gcttttttac ctcttttttc aacatgttga aattcgccag
    73201 acagtgtctg gcgaattaac acctgccctg gtccacttac gtccgtttac tttccgttaa
    73261 aaacgctgac acccgcagct tttatgaaaa agagacgctc cgctgtggct ggtctgttcg
    73321 ccagctagaa cggcaaattg cgacccaatt ttatgagcga acactgctgt cacatgacaa
    73381 atcagccatg ctgcaacaac acgctcctgc cgagacgcat attcttccgc aacaggcgat
    73441 acgcgatccc tttgtgctcg aatttctgga attgaaagat gaatactcag aatccgattt
    73501 tgaggaggcg ctgatcaacc acctgatgga tttcatgctg gaacttgggg atgattttgc
    73561 ctttgttggt cggcagcgaa ggttacgcat tgatgacaac tggtttcgtg tcgatctgct
    73621 gtttttccac cgccgtttac gctgcctgct aatcgtcgat ctaaaagtgg gcaaattcag
    73681 ctatagcgat gccggacaga tgaatatgta tctcaactac gccaaagagc actggacgct
    73741 accggatgaa aatccgccca tcggtctggt tctctgtgca gagaaaggag tcggagaagc
    73801 gcattatgca ctggctggtt tgcctaacac cgttctggcg agtgaatata agatgcaact
    73861 acctgatgaa aaacgactcg cggatgaact cgttcgaaca caggcggtgc tagaagaagg
    73921 ctatagactc cgttaatttc agcggtttcg aattctgcag acactgtctg cagaatcttt
    73981 aaaacgaact aagttttaag ccattaaatc agccttaacc ttcaccacca cctttttaat
    74041 ctcaccaggt tcgccgacaa cgcatcctgg tttatgcggt tctcccggca taaacacggc
    74101 gaacattccc ggttttaagt tgatggtttg ctcgttttca atggcgctgc aaagctggta
    74161 atcatcttca tggtggaact cttcacactg gcgcgcagtg cctgccgtgc caaacagaat
    74221 ccgttcctca ccttttaata acagctggat atcaatgtat tgctcgtgca attccgcttt
    74281 tttctcgacg ggcgattgag tgttaaacgt catgacattc ataaaaatat tgtcgccctg
    74341 taattcgtaa cgacccggcg ctttttcttg cggtctggca gctaatgcca gcgttaacgc
    74401 gtcctgtaac gcaggatgta acccagcaga cggtaatgac tgtacttcac ccatcatcat
    74461 aatttttctc cctgggccaa cagcgcagcc ccaagtaaac ctgcatcatg gcggtagtgc
    74521 gccgccagta aatcaacatg aaatgccgct ggctcctgcg ccagatacgt ttccaccagc
    74581 gccagatacc cttctgccag accaacgctg ccaccgacca ccacgcactg acaatcagtt
    74641 gtggctttaa tatcagcgat cagccttgcc agcacatgtg cggagcggtg aatcagctgc
    74701 tgcgcctgct cgtcgccctg cccggcgcgc atgaaaatag ttttcgcatc cgcgccagcc
    74761 aactccccct gcgctgccgc tgcaataccg cgaccagagg caatcgcttc cacgcaacct
    74821 gtgcgtccac agccgcagac tgggccgtgt ggatcggcaa gcgtatgccc gatgtgcccc
    74881 gccagaccgc cagggccggt aagcagtttg ccgccgctca ctacaccgcc gccgacgcca
    74941 gtggaaacgg tgataaacac catatcggtt atatcgcctt ccagcgcctg atactctgcc
    75001 catgctgcgg cctgggcgtc gttaatggca atggtcggca aatcggtaag ttgccccagc
    75061 gttttgacta acggaaagtg tagcaatcca ccaagattat gcggattcag cgccagcaaa
    75121 ctgccgtcgc gaataattcc ggttgaagcg atggcaaccc gctgcgcatg agcttgcaac
    75181 ggagagacta atgcggataa ggcatcacgc aaggcttctg gtgtctggct ggctggcgta
    75241 ggaagttcac gacgatcgcg gatctgcccg tcagcgccaa tcagcgcggc ggcaagttta
    75301 gtaccgccga tatcaatcgc cagtgtggtc atagcaccgc ctttttcatc gctgtgttgt
    75361 accactgaca aatgtgttca agacgcgtaa tggcagaacc gaccgtcacc gcccacgcgc
    75421 cgtggcgcat cgcatcagcc gcctgagcag gcgtgttgta acgcccttcg gcaatcaccc
    75481 gacatccggc gtcgctcaac cttttcacca gcgtcagatc cggctcttct ggcgtttcag
    75541 gcgtggtata gccagaaagc gtagtgccaa taatttcggc tcccagcttt tggcatgcca
    75601 ggccgtcttc cggcgttgag cagtcggtca tcgccagtaa accgtgatga tgaatacgtg
    75661 ccagcagcgt ttcaacaggc accggacgcg ggcggtcggt gccgtcaatg gcgataatgt
    75721 ccgcgcccgc ctgcgccagc gcatcgacat cttcaatata ggccgtgatg cgtaccggag
    75781 aatcctccag atcgcgtttc acaattccaa taatcggcac gctcaccacc gcacgcgtgg
    75841 cttgcagatt tgccacacct tcgatgcgaa tggcaaccgc gcccgcctgc tctgccgcta
    75901 atgccatggc tgcgacgatt tcgggtttat cgagcgggct gtccggaacc ggctggcagg
    75961 agacaatcag gccaccgtta gcagcgattt tttgatccag ttgtgcaagt aacgacatac
    76021 atcttccctt agcgaaaggc ccgatacaga gaccgggcaa caggattaac ttttggtttt
    76081 gactaaatcg tttttggcgc tgccaaacgg cacggcaccg ctaaatggtt tgccgtcgat
    76141 agcgtcatga gtacgcaacg cttccgggcg cagccaacgc tgaacacgag aaggcatatc
    76201 cagaccaatc agcaggatca ccacaaatgt cagactgaac gagagcgatg ccagtgcagt
    76261 acccagatcc agacgttgag cgatcaacgc gccgatgatt ggggccagtg cgccacccaa
    76321 tgcgccaacg ttataggtaa agcccaggcc cgctgcacgc tggtcggtat cgaaataacc
    76381 gccaatcagt ttcggtaaga tcccggcgat cccttgtcca agcatttgct ggaagaacag
    76441 taacagaccg agcacccaga cgtttgcacc gccaatagca aataccggaa taatcagcag
    76501 ctgcgaggcc aacagactac aaacgtacgc tttgcgggtt cccagccagt caccgaggaa
    76561 gccaccgacg cagcacccca ccgccgcgcc aaagccacta aagaacagca cattggcgac
    76621 agtatgaggg ttataagcca gatcggtttt cagatacgtt ggcagcagcg cctgaatcgg
    76681 ccatgagtag aggaaagcaa acaacacgac caccatcagc attacgccag ttggccagcg
    76741 tttgcccgta ctctgcacca taaagctgat aaagattgcg gtgcataaca gcccaagaac
    76801 agcgacgatc gcggcatttt gcaggttacc ggcgaagcag aaccacagcg cagtagccgc
    76861 cgccagtgtc attacgatat tggcaatgcg atgttcacca cggtagagaa tatccaccat
    76921 tgtgcgtact ggtgctttac ctgcgtgttt ctctttccag tcttctgctt ccgggatgtt
    76981 tttacgcagc cagagagcaa agatgattgg caaaatgccg ataaagaaca gcgcacgcca
    77041 gccccagacc ggaaccacca ggctgtagac ctgagcggca acgacggccc ctacagagaa
    77101 gcctgaaatc agaaagccac tggctttgtt acgcagatgt tttggccagc tttcaatgac
    77161 ataggtggcg ctggaaccgt attcacccgc catccccatg ccgatgacca gacgagcgat
    77221 aaacatggtg atgtagcctg gcgcaaagcc gcatgccagc gtcccggccg agaagagaac
    77281 gatgctggtg accattgcca gacgacgccc gtagcggtca cccatagcgc cgagcatcag
    77341 gccgccgaac cagcgagaga taaaggctgc ggagatcaga cttgccgcct gcaccgtcgt
    77401 cagcccgaat tcaccttgta cttcggtgag taccagggcg attaaaacga aatcaaaacc
    77461 gtcaagcaga tatcccaacc aggcagcgga aaatgcccgc cattgtgcac gattgagatg
    77521 gcgataccac gggatattct gggttgtagt actcattgtg agtctcccgc ggtgggcgat
    77581 gcccacacgc tttggtatga aaattgtagg gtacagatgc gtttatttcc cctcacccgg
    77641 taggggcgag cgaggggaaa caactcaccc gcgctcttgc atcaactgct gggccagcgc
    77701 cttcagttct ggcagatatt tttcatctac cggaccaaac ggtttgcggc acagcggcac
    77761 agaaacgaca tccatataat gcagaacagt tttcaggccg cggaatacac ccgttttgat
    77821 cagtaaatca atgactttgt tgcattcagt ttgcagtttc tgcgcggtct ggatatcgcc
    77881 ttctttcagc gccttaacga tcccctgata gcgccagccc atgatgttgt aggtactacc
    77941 gataccacca tcagcgcccg ccagcaggcc agaggcgaag atttcgtcgt aaccgttata
    78001 gagcaccaga tcaggatgtt cacgacggat ctgctccatc tgatagagat cgccagaggt
    78061 ctgtttcagc gcacctacgc caggcaatgt aacaagtgtg ttgatctgat ccagggtcag
    78121 ttttacccca ctcagggctg gaatgttgta caccaccatc ggcaaaccat ccgccgaatc
    78181 aataattgcc cgatagtgat cgcagtgttc ttcaaagctg aaaggatagt agaacggcgt
    78241 gacggcggag acggcatcga agccataacg tttagccgat gccgcaagtt gttggctttc
    78301 ggcggtgctg acgcaaccga cgtgggcgat gagtttaatc ttacctttcg cctcttcggc
    78361 gacgatttcc agtacctgtt cacgctcgga aaggctttgt acaaaggctt cgccggtcga
    78421 accacccacg tataaaccgt caatgccctg ctgaatattg aactgaacca ggcggcgcag
    78481 actcgcttta tccagtgctt gttgttgatc aaaaggagtc aggagtgcag ccattacgcc
    78541 acgtaaattc gttgccataa atacctctga agtgatgctt gtctgataaa cgatatacct
    78601 ttatacctgt tataccagat caattaagca acaccccata cagaaagctt ataatgcgat
    78661 ctgcttcact aaagtggcat tatttctttt tgttggtggt ctgaccgaag gcgtgccagg
    78721 tagcagagac gctgttgaga tgcgattgca acgcacgatc ggcttcgtca ggatcgtggc
    78781 ggcggatcgc gtcaacgatc gcaatatgct gttgatagct aacgttgtta tgctcgtgca
    78841 gagcctgatc agccacggtt ggacgtgcgg cgatcagcca gtcgagcagg gcaacgtgga
    78901 tcgccatgaa gattgggtta ccggggatct ccgccagcac gcggtggaaa tcaacgtctg
    78961 agcgaatgaa tgccgcgttg ttatccagcg actgactgtt gatttccagt gcttttgcca
    79021 gcaaatcgat ttgctcatcg gtggcgtgtt cagccgcata gcgcaccagg ctggattcaa
    79081 agaacagacg taattgttcg aaatgggcaa tcccaccagg atgagaaagg aaatctttcg
    79141 ccatgccgga aagctcgccg atgatggtgt ccgcagaagg acgtgagacg cgggcacgtt
    79201 cgccgttgtt tatttgcacc agacctttgc gttttaacgc tgccagcgct tcacgcaccg
    79261 aaggacgccc gacgttaaag aacgccatca gttcgcgttc agacggtaat tgttcacctt
    79321 cgccaaattc acgacggcgg atcatctgtt ccagctcttc ttccaccatt tcggagagtt
    79381 ttttacgcgc cagcgggcgg ctacgcaagt tgcgaccaat tgcaggtgaa gaatcttcgg
    79441 tttgcgaatc aaatgcgttc ataaggccca ttctgtaagg tcagtgtgat taacatcatc
    79501 agtgacatcc tatcacagga ttgaaagtag gggaaaatgg cagggttttc tctttgtgcc
    79561 tcatcattac cataattaac ggaataatta actattgcga aaaattaatg taacgcagat
    79621 aaaaacatcc cgtttgaatt atttataaga ctattcacga gcattatgaa tattatgaat
    79681 gtgttcttac aaaataatca taagcgcata ttttttaatg aaaaatcacc tcacctacaa
    79741 ttaaaaacac gacatccgca ccataaatag acttgcaaaa aatataacat cattgttttc
    79801 aatctgccgt ttatgggatt gacccttttc ttttgacacg gagttcaaca atgttcggca
    79861 taattatatc tgtcatcgta ttaattacga tgggctattt gatcctgaaa aactacaaac
    79921 ctcaggtggt gctggctgca ggtatcttcc tgatgatgtg cggtgtctgg ttagggttag
    79981 gtggtgtact cgatcccgcc aaaagcagcg gctacttgat cgtcgatatt tataatgaaa
    80041 tcctgcgcat gctgtccaac cgcattgccg gattggggct gtcgattatg gcggtgggcg
    80101 gttatgcccg ctacatggag cgcacaggag ccagtcgcgc gatggtgagc ttgttaagcc
    80161 gcccgttaaa actcattcgc tcgccgtata ttattctgtc ggcaacttac gtcatcggcc
    80221 aaatcatggc gcagtttatt accagcgcct ccggcctggg tatgttgctg atggtcacct
    80281 tatttccgac actggtgagt ctgggagtaa gccgtctctc tgcggtggcg gttatcgcaa
    80341 ccacaatgtc cattgagtgg gggattctgg aaacgaactc catttttgca gcccaggtag
    80401 cgggaatgaa aattgccact tacttcttcc actaccagct tccggtcgcc tcttgcgtca
    80461 ttatctcggt ggcgatctcc cactttttcg tgcaacgcgc ttttgacaaa aaagataaaa
    80521 atatcaatca cgaacaggca gagcaaaaag ctctcgataa tgtcccgccg ctctattacg
    80581 ccattttacc tgtgatgccg ttaatcctga tgctcggctc gctattcctt gcccacatcg
    80641 ggctgatgca gtcagaactg catctggtgg tggtgatgtt actgagtttg acgatgtttg
    80701 ttgagttctt ccgcaagcat aacttgcgcg aaacaatgga cgatgtgcag gcgttttttg
    80761 acggcatggg tacgcagttt gccaacgtgg taacgctggt ggtcgcgggt gaaatatttg
    80821 cgaaaggctt aacgacgatt ggcactgtcg atgcggttat caggggtgcg gagcattctg
    80881 gtctgggcgg tattggcgtg atgattatta tggcgctggt cattgccatt tgtgccattg
    80941 tgatgggctc tggcaatgcg ccgtttatgt catttgccag tcttattccg aatatcgcag
    81001 ccggactaca tgtaccagcg gttgtaatga ttatgccgat gcattttgcc acgacgctag
    81061 cgcgcggttt cgccgattac tgcggtggtg gtcgttacgt caggaattgc aggcgtttcg
    81121 ccttttgcgg tggtgaagcg gacagcgatc cccatggcag tcggtttcgt ggtgaatatg
    81181 attgccacaa tcacgctatt ttattaagtc attaaaagac aaaacaggcc gccagggcct
    81241 gtttttgtat tacttcacaa cgcgtaatgc cggtcgacca ccgcgtggtg gctgcggtgg
    81301 ttcatcgtca ggatgagtgt catcatcgtg atctggcttg tcgccatcaa taaccgacat
    81361 aacggtttcg ttgtctgccg atgcctcttc atcattcatg atgctggtat cttcatcgta
    81421 ggcagcttca ggctcaaaca tcgtgcctgc gccattttca cgggcgtaga tagccagcac
    81481 ggcagccagc ggcacagaaa cctgacgcgg aatgccacca aagcgcgcgt taaagcgcac
    81541 ctcatcattc gccagttcca gattgccgac agcacgcggc gcaatgttga gtacgatttg
    81601 cccgtcacgc gcatattcca taggaacctg cacgccaggg agcgtcacat ccaccaccag
    81661 gtgcggcgtg agctggttat ccagcaacca ctcatagaat gcacgcagca gatagggacg
    81721 acgtggtgtt agctgtgaca aatccataca gattaactcc ggcccagacg catttcacgt
    81781 tctgcttcag ttaaagaagc aaggaaagag tcacgctcaa agacgcgggt catatagcct
    81841 ttcagctctt tcgcacccgg gccgctgaac tcgataccca gttgcggcag acgccacagc
    81901 agcggagcaa ggtagcaatc gaccaggctg aactcatcgc tcaggaagta cggcttctga
    81961 ccgaagaccg gcgcaatcgc cagcagttct tcgcgcagtt gcttacgtgc ggcatctgct
    82021 tcagaagctg aaccgttgat gatggtgttc atcagcgtgt accagtcttt ttcgatgcga
    82081 tgcatgtaca aacggctttc accgcgagct accgggtaaa caggcatcag tggcggatgc
    82141 gggaaacgct catccagata ttccataatg atgcgagatt cccacagggt cagctcacga
    82201 tccaccaggg tcggaacgct ctgattcggg ttgaggtcaa tcagatcctg aggcggattg
    82261 tccttttcca cgtgttcgat ctcgaaactt acacctttct cagccagcac aatgcggacc
    82321 tgatggctat agatgtcagt aggaccggaa aacagcgtca ttaccgaacg tttgttggca
    82381 gcgacagcca tgaaaacctc caggtatagt cagaattttt actgctacca gccaccaggt
    82441 ggccagtcag aagttgtgtt acccagtaag gaacgactct ctttgttcga aaatcaaaca
    82501 aaaaatgagc aatacccgac atttgggcag aaaattggat gatagtttac cagattttgc
    82561 gacctttgtg gtgagtcgat gccggaaatg gggaaaaaga gatgcgcttt agtctgaaat
    82621 agttgactta gtcccttatt ggcgatgtgg tttttgtttt gcctgtctgt cagacggcag
    82681 caaaaagcaa ctttccattt tttacgctga ttcagaattt aaccataaaa aaacccgccg
    82741 aagcgggttt tttcgcaaat tgttttctgc cggagcagaa gccaattaac gtttggagaa
    82801 ctgcggacga cgacgtgctt tacgcagacc gactttctta cgttcaacct gacgagcgtc
    82861 acgagtaacg aagccagctt tacgcagttc agaacgcagg gactcgtcgt attccatcag
    82921 agcgcgggtg ataccgtgac ggatcgcacc agcctgacca gagataccac cacctttaac
    82981 ggtgatgtac aggtccagtt tctcaaccat atcgaccagt tccagcggct gacgaactac
    83041 catgcgagca gtttcacgac cgaagtactg ttccagagaa cgttggttga ttacgatttt
    83101 accgttgccc ggtttgatga aaacgcgagc tgcggaactt ttgcggcgac cagtgccgta
    83161 gtattgattt tcagccattg cctataatcc cgattagatg tcaagaactt gcggttgctg
    83221 tgccgcgtgg ttgtgctcgt tacccgcgta aactttcagt ttacggaaca tagcacgacc
    83281 cagcgggcct tttggcaaca tgcctttaac cgcgatttca atcacacgct caggacggcg
    83341 agcaatcatc tcttcaaagg tcgcttgttt gataccaccg atgtggccgg tgtggtgata
    83401 gtacactttg tcagtacgct tgttgccggt tacagcaact ttgtcagcgt tcagaacgat
    83461 gatgtaatca ccggtatcta cgtgcggagt gtattccgct ttgtgcttac cgcgcaggcg
    83521 acgagccagt tcagtagcca gacggcccag agttttaccg gtcgcgtcaa caacatacca
    83581 gtcgcgtttt acggtttctg gtttagctgt aaaagttttc attaaaagct tacccaataa
    83641 atagttacac gttggtgaac acccaaacgt cttcaattgt tgaggttcac acgacaaagt
    83701 ccggcaaacc taccccttcg aatagcctat gccagcacac aaaaagtttt gggaaaaaaa
    83761 ctttcttgta acgtggggtc gcaggattat agagaagtcg gggtcaaaga tcgacccctt
    83821 tttgtgattt gtggcaggtt ttaacccgct aaatgctcgc gcttcagata ctcttcgctt
    83881 tgcatctctt gcagacgtga caggcaacgc tggaactcaa acttcagccg atcgccctga
    83941 taaatttcat acagcggcac ttctgcactc accactaatt tgacatggcg ctcgtaaaac
    84001 tcatccacca gcgcaataaa gcgccgcgct tcgctctcca tcaaccgcgt cataactggt
    84061 acatcaaaca acatgaccgt atgaaagaga cgtgagagcg caatatagtc atgctgactg
    84121 cgggcgtcga cgcacagcgt agtaaaagag accgccagcg tctggttctc gacgcccatt
    84181 gtcgctaatg gccgatggtt gatttctaac gtcggtgaat tttctcgttt cccccccgcc
    84241 agcgccaacc atagtttatc catttgcgcc cgggtttcat cgtggagtgg cgaaagccac
    84301 agatgcgcct gagtgagtgt acgcagacga taatcaacac cagcgtccac gttcattaca
    84361 tcacaatgct gtttaatggc atcgattgca ggcagaaaac gcgcacgttg caagccattt
    84421 cgataaagtt cgtccggcgg aatatttgac gtcgctacca gggtaatacc gcgggcgaac
    84481 agggctttca tcaggccgcc aagtagcatg gcatcggtaa tatcagagac aaaaaattcg
    84541 tcaaaacaga gcacgtcagt ttctgcttta aaacgatcag caataatttc cagcggatcg
    84601 gtctgcccct gtaaggcagt tagctcttca tgcacccgca gcataaaacg gtgaaagtgc
    84661 aggcgctgtt tccgctctcc cggcaggctt tgatagaaaa ggtccatcag ccaggttttc
    84721 ccgcgtccta caccgcccca catatataag ccacgcactg gcgtatgctt tgtgtcttcc
    84781 cgtttacccc acagcttacc gacccgcgcc attagcccac tcgtcctggg ggctggtggc
    84841 gtgctattga tgagttcctg ataaataatt tccaagcggc tgacggcctc tttttgaacg
    84901 tcgtcgggtt gatggctgcc ttcattgagc gccttcaggt attgcgatgt tggagtaacg
    84961 ctttgcatga tcttattgtt attccttgaa taatcggtgc gccgttgttc acggttgacg
    85021 aaaaaaaggc cgttctacac tacgcgatat gcagtcggga ttccacttct gtggaattaa
    85081 cggttatagt ggcataatca gccgcaggca tggagcctga agccaacacc ctacggaaac
    85141 aaaagacaac gggagatgtt catgacctgg gaatatgcgc taattgggtt agtcgtcggc
    85201 atcattattg gtgctgtggc catgcgtttt ggtaatcgta aactacgcca gcaacaggcg
    85261 ttgcagtacg aactggaaaa gaataaagct gaactggacg agtatcgcga agagctggtt
    85321 agccactttg cccgcagcgc ggaattactg gataccatgg cgcacgacta tcgccagctg
    85381 tatcagcaca tggcgaaaag ctccagcagc ctgctgccgg aactgtctgc tgaagcaaac
    85441 ccgttccgta atcgtctggc agagtctgaa gccagcaacg atcaggcacc ggtgcagatg
    85501 cctcgcgact attctgaagg cgcatccggc ctgctgcgta ctggcgcgaa gcgcgactaa
    85561 tttatttttc gggcgcagcc attgcgccct cctcttctct ccctccccga ctatcattta
    85621 atctggtgtc tcattgttag ccgtctgaaa attcaataac atcaaactgt tttgaatctc
    85681 ttttcttatc attcaggtac gagagcagga ataatgaaaa aacaaaccca gctgttgagt
    85741 gcattagcgt taagtgtcgg gttaactctc tcggcgtcat ttcaggccgt tgcgtcgatc
    85801 ccaggccagg ttgccgatca ggcccccctc cccagtctgg ctccaatgct ggaaaaagtg
    85861 cttccggcag tggtgagcgt acgggtggaa ggaacggcca gtcagggaca gaaaatcccg
    85921 gaagaattca aaaagttttt tggtgatgat ttaccggatc aacctgcaca acccttcgaa
    85981 ggtttaggct ccggtgtcat catcaacgcc agtaaaggct atgtgctgac caacaaccat
    86041 gtgattaatc aggcacagaa aatcagtatt cagctcaatg atgggcgcga gtttgatgca
    86101 aaactgattg gtagcgatga ccagagcgat atcgccctgt tgcaaattca aaacccgagc
    86161 aaattaacgc aaatcgctat tgccgactcc gataaattgc gcgtcggtga ttttgccgtg
    86221 gcggtcggta acccatttgg ccttgggcaa accgccacct ctggcattgt ttccgcatta
    86281 ggacgcagcg ggttgaatct tgaaggtctg gaaaacttta tccagacaga tgcttccatt
    86341 aaccgcggta actccggcgg tgcactgtta aaccttaacg gtgagttaat tggcatcaac
    86401 actgcaatcc ttgcgcctgg cggcgggagc gtcggaattg gatttgccat ccccagtaat
    86461 atggcgcgaa cactggcgca gcagcttatc gactttggtg aaatcaaacg cggtttgtta
    86521 ggcatcaaag gcaccgagat gagtgccgat atcgccaaag ccttcaacct tgacgtgcag
    86581 cgtggcgcgt ttgtcagcga agtgttgcca ggttctggct cggcgaaagc gggcgtcaaa
    86641 acgggcgata ttattaccag cctcaacggc aaaccgctga atagctttgc tgagttgcgc
    86701 tctcgtatcg cgaccaccga gccgggcacg aaagtgaagc ttggcctgct gcgtaacggc
    86761 aaaccactgg aagtagaagt gacgctcgat accagcacct cttcgtcggc cagcgctgaa
    86821 atgatcacgc cagcgctgga aggtgcaacg ttgagcgatg gtcagctaaa agatggcggc
    86881 aaaggtatta aaatcgatga agttgtcaaa ggaagcccag ctgctcaggc tggcttgcaa
    86941 aaagacgatg tgatcattgg cgtcaaccgc gatcgggtga actcgattgc tgaaatgcgt
    87001 aaagtgctgg cggcaaaacc ggccatcatc gccctgcaaa ttgtacgcgg caatgaaagc
    87061 atctatctgc tgatgcgtta atgtcgtaaa ccgggcatca ggctgacgtg tgatgtccgg
    87121 ttaactcgtg gtatgctgct gccgttccct tttttaatga cgcctccatc atgtttgtga
    87181 agctcttacg ttccgttgcg attggattaa ttgtcggcgc tattctgctg gttgccatgc
    87241 cttcgctgcg cagccttaac ccgctttcca ctccgcaatt tgacagtacc gatgagacgc
    87301 ctgccagtta taatctggcg gttcgccgcg ccgcgccagc ggtggttaac gtttacaacc
    87361 gtggtttgaa caccaactct cacaaccagc ttgagatccg caccctggga tccggtgtaa
    87421 tcatggatca acgcggttat atcatcacca ataaacacgt catcaacgac gccgatcaga
    87481 tcatcgtcgc cttacaggat ggacgtgtat ttgaagcatt gctggtggga tctgactctc
    87541 taaccgatct ggcggtactt aaaattaatg ccactggcgg tttacctacc attccaatta
    87601 atgcacgtcg cgtaccgcac attggcgacg tggtactggc gatcggtaac ccgtacaacc
    87661 tcgggcagac cattacccag gggattatta gtgctacggg tcgaatcggt ctgaacccga
    87721 ccgggcggca aaacttccta caaaccgatg cttccattaa ccacggtaac tctggcggcg
    87781 cgctggtgaa ctcgctgggc gaactgatgg gcattaacac gctgtcgttt gataagagta
    87841 acgatggcga aacgccggaa ggtatcggct ttgcgattcc tttccagtta gcaaccaaaa
    87901 ttatggataa gctgatccgc gatggtcgcg tgatccgcgg ctacattggt atcggcggac
    87961 gcgagatcgc accactgcac gcgcagggcg gtggtataga tcaactgcaa gggatcgtgg
    88021 ttaatgaagt gtcacctgac ggcccggcgg cgaatgcggg tattcaggtc aacgatctga
    88081 ttatttcggt ggataacaaa ccggccatct ctgctctgga gacgatggat caggtggcag
    88141 aaattcgccc tggttcggtg atcccggttg tagtgatgcg tgatgataag cagttaacgc
    88201 tgcaggtcac cattcaggaa tatccggcaa ccaattaagg ccagcgtgaa agacaaaaaa
    88261 accggagtct gtgctccggt tttttattat ccgctaatca attacttatt aacgaactct
    88321 tcgcccaggg cgatatcttt cttgagcgta tccagcatac cttccagcgc gttctgttca
    88381 aatgcgctca gggtaccgat agatttacgc tcttccacgc cgtttttacc cagcagaagc
    88441 ggctgagaga agaaacgggc gtactgaccg tcgccttcaa cgtaggcaca ttcgacaacg
    88501 ccttgttcgc cctgcagtgc gcgaaccaga gacaggccaa aacgtgcagc agcctggccc
    88561 atagacaggg ttgccgaccc gccaccggct ttcgcttcaa ccacctcagt acccgcgttc
    88621 tggatacgtt tggtcagatc agccacttcc tgctcggtaa aactaacgcc aggaacctgt
    88681 gacaacagcg gcagaatggt aacaccagag tgaccgccaa taaccggcac ttcaacttcg
    88741 cctggctgtt tgcctttcag ttccgcaaca aaggtgttgg aacgaatgat atccagcgtg
    88801 gtaacgccga acagtttgtt tttgtcataa acaccggctt ttttcagcac ttcagcagca
    88861 atcgcaactg tggtgttaac cgggttagtg ataataccaa tgcacgcttt cgggcaggtt
    88921 ttcgcaactt gctgtaccag gtttttcacg atgccggcgt taacattaaa caggtcggaa
    88981 cgatccatac ccggtttacg cgctacacct gcagagataa gaacgacatc tgcgccttcc
    89041 agcgccggag tcgcatcttc accagaaaaa cctttgattt tcacagcagt agggatatgg
    89101 ctcagatcga cggccacacc gggagtcact ggagcgatat catacagaga gagttctgaa
    89161 cctgaaggca gttgggtttt taacagtagt gcaagcgcct ggccaatacc gccagcagcg
    89221 ccgaggactg cgactttcat cctaaactcc ttattatatt gataaactaa gatatgttgc
    89281 tccgctgccg cgaccttaat ccacaaaatt gccgtttaca atgaccacat ctcaagaatg
    89341 tgtagtcacg caagtttagc gtttatgcat ttaattgccg taatcaggaa cctaagttac
    89401 gtaattaaca atcgcttatg cattagcgca acattccaac aggtggtgac aatataccct
    89461 accgttcagc caaaacaaca tcaatttgat aacaattaat ttacttttaa gcagaatttg
    89521 catgccgtga cgcaggcata tttctcaata acgaaatttg ataaaatccc gctctttcat
    89581 aacattattt cagccttctt cagggctgac tgtttgcata aaaattcatc tgtatgcaca
    89641 ataatgttgt atcaaccacc atatcgggtg acttatgcga agctcggcta agcaagaaga
    89701 actagttaaa gcatttaaag cattacttaa agaagagaaa tttagctccc agggcgaaat
    89761 cgtcgccgcg ttgcaggagc aaggctttga caatattaat cagtctaaag tctcgcggat
    89821 gttgaccaag tttggtgctg tacgtacacg caatgccaaa atggaaatgg tttactgcct
    89881 gccagctgaa ctgggtgtac caaccacctc cagtccattg aaaaatctgg tgctggatat
    89941 cgactacaac gatgcagttg tcgtgattca taccagccct ggcgcggcgc agttaattgc
    90001 tcgcctgctg gactcactgg gcaaagcaga aggtattctg ggcaccatcg ctggcgatga
    90061 caccatcttt accacccctg ctaacggttt caccgtcaaa gacctgtatg aagcgatttt
    90121 agagctgttc gaccaggagc tttaatctct gccccgtcgc ttctggcggc ggggaaaatg
    90181 ttgcttatcc ctctcaaccc cctgctttcc cctgcgatta atttaacgaa tagtgcgttt
    90241 tactgcgaca tgtcattcac acaatgaata cataaggtaa aaaaagcaca ttatgcaaaa
    90301 ttcattatct aattgaaaaa actagaatta acgataaata accgtat
//
LOCUS       DAZFAB010000006        88963 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZFAB010000006 DAZFAB010000000
VERSION     DAZFAB010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 88963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 88963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..88963
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.006"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            37..768
                     /locus_tag="ACHJWA_000506"
     CDS             37..768
                     /locus_tag="ACHJWA_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO4307171.1"
                     /translation="MKKWKVRSALVALIVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     TVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGQAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            904..1824
                     /gene="speB"
                     /locus_tag="ACHJWA_000507"
     CDS             904..1824
                     /gene="speB"
                     /locus_tag="ACHJWA_000507"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFO4307172.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(2030..2788)
                     /gene="loiP"
                     /locus_tag="ACHJWA_000508"
                     /gene_synonym="yggG"
     CDS             complement(2030..2788)
                     /gene="loiP"
                     /locus_tag="ACHJWA_000508"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFO4307173.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            3066..5057
                     /gene="tkt"
                     /locus_tag="ACHJWA_000509"
     CDS             3066..5057
                     /gene="tkt"
                     /locus_tag="ACHJWA_000509"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO4307174.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            5371..5814
                     /gene="cmtB"
                     /locus_tag="ACHJWA_000510"
     CDS             5371..5814
                     /gene="cmtB"
                     /locus_tag="ACHJWA_000510"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFO4307175.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            5842..7230
                     /gene="cmtA"
                     /locus_tag="ACHJWA_000511"
     CDS             5842..7230
                     /gene="cmtA"
                     /locus_tag="ACHJWA_000511"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFO4307176.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            7245..8522
                     /gene="yggP"
                     /locus_tag="ACHJWA_000512"
     CDS             7245..8522
                     /gene="yggP"
                     /locus_tag="ACHJWA_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO4307177.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVELAASKGIELVYVNTKGMSDPVQMLRALTGDAGFDDIFVYAAVSAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            8519..9484
                     /gene="glpX"
                     /locus_tag="ACHJWA_000513"
     CDS             8519..9484
                     /gene="glpX"
                     /locus_tag="ACHJWA_000513"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFO4307178.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRDVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGNMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDVLVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLY"
     gene            9506..10015
                     /gene="fumE"
                     /locus_tag="ACHJWA_000514"
     CDS             9506..10015
                     /gene="fumE"
                     /locus_tag="ACHJWA_000514"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFO4307179.1"
                     /translation="MATLSEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEESVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLKEQD
                     ETLGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            10012..10725
                     /locus_tag="ACHJWA_000515"
     CDS             10012..10725
                     /locus_tag="ACHJWA_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFO4307180.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWAYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPIIIVEGNWLLLDDDN
                     WRQLAQFCDFSIFINAPATALRERLVGRKLAGGLSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(10697..11374)
                     /locus_tag="ACHJWA_000516"
     CDS             complement(10697..11374)
                     /locus_tag="ACHJWA_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO4307181.1"
                     /translation="MLTLNQISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKIGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTASAHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGLIRNIN
                     PPDDIHP"
     gene            complement(11368..12045)
                     /locus_tag="ACHJWA_000517"
     CDS             complement(11368..12045)
                     /locus_tag="ACHJWA_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO4307182.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(12033..12740)
                     /locus_tag="ACHJWA_000518"
     CDS             complement(12033..12740)
                     /locus_tag="ACHJWA_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFO4307183.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWTHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALTIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            complement(12741..13319)
                     /locus_tag="ACHJWA_000519"
     CDS             complement(12741..13319)
                     /locus_tag="ACHJWA_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFO4307184.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKTLTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(13342..13773)
                     /locus_tag="ACHJWA_000520"
     CDS             complement(13342..13773)
                     /locus_tag="ACHJWA_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HFO4307185.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQQQLHAAWIA
                     GCTGSLTDIALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            14145..15164
                     /gene="epd"
                     /locus_tag="ACHJWA_000521"
     CDS             14145..15164
                     /gene="epd"
                     /locus_tag="ACHJWA_000521"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFO4307186.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            15214..16377
                     /gene="pgk"
                     /locus_tag="ACHJWA_000522"
     CDS             15214..16377
                     /gene="pgk"
                     /locus_tag="ACHJWA_000522"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFO4307187.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDEILSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            16592..17671
                     /gene="fbaA"
                     /locus_tag="ACHJWA_000523"
     CDS             16592..17671
                     /gene="fbaA"
                     /locus_tag="ACHJWA_000523"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFO4307188.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(17735..17833)
                     /locus_tag="ACHJWA_000524"
                     /pseudo
     CDS             complement(17735..17833)
                     /locus_tag="ACHJWA_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            18029..18889
                     /gene="mscS"
                     /locus_tag="ACHJWA_000525"
     CDS             18029..18889
                     /gene="mscS"
                     /locus_tag="ACHJWA_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFO4307189.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            19028..19663
                     /gene="argO"
                     /locus_tag="ACHJWA_000526"
     CDS             19028..19663
                     /gene="argO"
                     /locus_tag="ACHJWA_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFO4307190.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            19756..20496
                     /gene="yggE"
                     /locus_tag="ACHJWA_000527"
     CDS             19756..20496
                     /gene="yggE"
                     /locus_tag="ACHJWA_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFO4307191.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            20663..21559
                     /gene="ygfI"
                     /locus_tag="ACHJWA_000528"
                     /pseudo
     CDS             20663..21559
                     /gene="ygfI"
                     /locus_tag="ACHJWA_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(21556..23034)
                     /gene="scpC"
                     /locus_tag="ACHJWA_000529"
     CDS             complement(21556..23034)
                     /gene="scpC"
                     /locus_tag="ACHJWA_000529"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFO4307192.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHNEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(23058..23843)
                     /gene="scpB"
                     /locus_tag="ACHJWA_000530"
     CDS             complement(23058..23843)
                     /gene="scpB"
                     /locus_tag="ACHJWA_000530"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFO4307193.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(23854..24849)
                     /gene="meaB"
                     /locus_tag="ACHJWA_000531"
     CDS             complement(23854..24849)
                     /gene="meaB"
                     /locus_tag="ACHJWA_000531"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFO4307194.1"
                     /translation="MINEATLAESIRRLRQGEHATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNTLRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(24842..26986)
                     /gene="scpA"
                     /locus_tag="ACHJWA_000532"
     CDS             complement(24842..26986)
                     /gene="scpA"
                     /locus_tag="ACHJWA_000532"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFO4307195.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKTGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIIAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEAAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGLDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(27190..28083)
                     /gene="argP"
                     /locus_tag="ACHJWA_000533"
     CDS             complement(27190..28083)
                     /gene="argP"
                     /locus_tag="ACHJWA_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFO4307196.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            28466..29125
                     /gene="rpiA"
                     /locus_tag="ACHJWA_000534"
     CDS             28466..29125
                     /gene="rpiA"
                     /locus_tag="ACHJWA_000534"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFO4307197.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            29380..30612
                     /gene="serA"
                     /locus_tag="ACHJWA_000535"
     CDS             29380..30612
                     /gene="serA"
                     /locus_tag="ACHJWA_000535"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFO4307198.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            30841..30900
                     /gene="ibsC"
                     /locus_tag="ACHJWA_000536"
     CDS             30841..30900
                     /gene="ibsC"
                     /locus_tag="ACHJWA_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFO4307199.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            31234..31293
                     /locus_tag="ACHJWA_000537"
     CDS             31234..31293
                     /locus_tag="ACHJWA_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO4307200.1"
                     /translation="MMRLVIILIVLLLISLPAY"
     gene            complement(31394..31996)
                     /gene="fau"
                     /locus_tag="ACHJWA_000538"
     CDS             complement(31394..31996)
                     /gene="fau"
                     /locus_tag="ACHJWA_000538"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFO4307201.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(32017..32200)
                     /gene="ssrS"
                     /locus_tag="ACHJWA_000539"
     ncRNA           complement(32017..32200)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHJWA_000539"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(32242..32571)
                     /gene="zapA"
                     /locus_tag="ACHJWA_000540"
     CDS             complement(32242..32571)
                     /gene="zapA"
                     /locus_tag="ACHJWA_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO4307202.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            32739..33317
                     /gene="ygfB"
                     /locus_tag="ACHJWA_000541"
     CDS             32739..33317
                     /gene="ygfB"
                     /locus_tag="ACHJWA_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFO4307203.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            33343..34668
                     /gene="pepP"
                     /locus_tag="ACHJWA_000542"
     CDS             33343..34668
                     /gene="pepP"
                     /locus_tag="ACHJWA_000542"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFO4307204.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECEMRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DIDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            34665..35843
                     /gene="ubiH"
                     /locus_tag="ACHJWA_000543"
                     /gene_synonym="visB"
     CDS             34665..35843
                     /gene="ubiH"
                     /locus_tag="ACHJWA_000543"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFO4307205.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLEGGETLTGRVLVA
                     ADGTHSALATVCGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTHAARS
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERGEDMGDYGVLCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWTPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            35867..37069
                     /gene="ubiI"
                     /locus_tag="ACHJWA_000544"
                     /gene_synonym="visC"
     CDS             35867..37069
                     /gene="ubiI"
                     /locus_tag="ACHJWA_000544"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFO4307206.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPVKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(37143..37226)
                     /locus_tag="ACHJWA_000545"
     CDS             complement(37143..37226)
                     /locus_tag="ACHJWA_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4307207.1"
                     /translation="MSKNTKSKIRDIRKYNAKTEVKLVYFK"
     gene            37517..38611
                     /gene="gcvT"
                     /locus_tag="ACHJWA_000546"
     CDS             37517..38611
                     /gene="gcvT"
                     /locus_tag="ACHJWA_000546"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFO4307208.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHEVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEGGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPKGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            38635..39024
                     /gene="gcvH"
                     /locus_tag="ACHJWA_000547"
     CDS             38635..39024
                     /gene="gcvH"
                     /locus_tag="ACHJWA_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFO4307209.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            39142..42015
                     /gene="gcvP"
                     /locus_tag="ACHJWA_000548"
     CDS             39142..42015
                     /gene="gcvP"
                     /locus_tag="ACHJWA_000548"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFO4307210.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDIFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPIGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(42181..43614)
                     /gene="bglA"
                     /locus_tag="ACHJWA_000549"
     CDS             complement(42181..43614)
                     /gene="bglA"
                     /locus_tag="ACHJWA_000549"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFO4307211.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINLEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDSDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQKPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGENL"
     gene            43659..43970
                     /gene="yqfB"
                     /locus_tag="ACHJWA_000550"
     CDS             43659..43970
                     /gene="yqfB"
                     /locus_tag="ACHJWA_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFO4307212.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELKKVIADIYPGQTQFYVIEFKC
                     L"
     gene            44134..44793
                     /gene="yqfA"
                     /locus_tag="ACHJWA_000551"
     CDS             44134..44793
                     /gene="yqfA"
                     /locus_tag="ACHJWA_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFO4307213.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(44870..45850)
                     /gene="ygfZ"
                     /locus_tag="ACHJWA_000552"
     CDS             complement(44870..45850)
                     /gene="ygfZ"
                     /locus_tag="ACHJWA_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFO4307214.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYLQGQ
                     VTADVSQMTDDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLAELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANIFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLTGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLCIEPLPYSLEE"
     gene            46093..46359
                     /gene="sdhE"
                     /locus_tag="ACHJWA_000553"
     CDS             46093..46359
                     /gene="sdhE"
                     /locus_tag="ACHJWA_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFO4307215.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            46340..46747
                     /gene="ygfX"
                     /locus_tag="ACHJWA_000554"
     CDS             46340..46747
                     /gene="ygfX"
                     /locus_tag="ACHJWA_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFO4307216.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     SGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(46787..47308)
                     /gene="fldB"
                     /locus_tag="ACHJWA_000555"
     CDS             complement(46787..47308)
                     /gene="fldB"
                     /locus_tag="ACHJWA_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFO4307217.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            47420..48316
                     /gene="xerD"
                     /locus_tag="ACHJWA_000556"
     CDS             47420..48316
                     /gene="xerD"
                     /locus_tag="ACHJWA_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFO4307218.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            48341..49051
                     /gene="dsbC"
                     /locus_tag="ACHJWA_000557"
     CDS             48341..49051
                     /gene="dsbC"
                     /locus_tag="ACHJWA_000557"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFO4307219.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            49057..50790
                     /gene="recJ"
                     /locus_tag="ACHJWA_000558"
     CDS             49057..50790
                     /gene="recJ"
                     /locus_tag="ACHJWA_000558"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFO4307220.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQIEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            50881..51979
                     /gene="prfB"
                     /locus_tag="ACHJWA_000559"
     CDS             join(50881..50955,50957..51979)
                     /gene="prfB"
                     /locus_tag="ACHJWA_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFO4307221.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            51989..53506
                     /gene="lysS"
                     /locus_tag="ACHJWA_000560"
     CDS             51989..53506
                     /gene="lysS"
                     /locus_tag="ACHJWA_000560"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO4307222.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIVESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(53549..54097)
                     /gene="idi"
                     /locus_tag="ACHJWA_000561"
     CDS             complement(53549..54097)
                     /gene="idi"
                     /locus_tag="ACHJWA_000561"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFO4307223.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            54152..54223
                     /locus_tag="ACHJWA_000562"
     CDS             54152..54223
                     /locus_tag="ACHJWA_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4307224.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(54220..54345)
                     /gene="yqfG"
                     /locus_tag="ACHJWA_000563"
     CDS             complement(54220..54345)
                     /gene="yqfG"
                     /locus_tag="ACHJWA_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFO4307225.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(54347..55795)
                     /gene="uacT"
                     /locus_tag="ACHJWA_000564"
     CDS             complement(54347..55795)
                     /gene="uacT"
                     /locus_tag="ACHJWA_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFO4307226.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAQLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            56231..58150
                     /gene="ygfT"
                     /locus_tag="ACHJWA_000565"
     CDS             56231..58150
                     /gene="ygfT"
                     /locus_tag="ACHJWA_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFO4307227.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGLAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKNHFGEIYCGLDPQQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            58150..58638
                     /gene="ygfS"
                     /locus_tag="ACHJWA_000566"
                     /pseudo
     CDS             58150..58638
                     /gene="ygfS"
                     /locus_tag="ACHJWA_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
     gene            complement(58674..60041)
                     /gene="ghxQ"
                     /locus_tag="ACHJWA_000567"
                     /gene_synonym="ygfQ"
     CDS             complement(58674..60041)
                     /gene="ghxQ"
                     /locus_tag="ACHJWA_000567"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HFO4307228.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(60077..61393)
                     /gene="guaD"
                     /locus_tag="ACHJWA_000568"
     CDS             complement(60077..61393)
                     /gene="guaD"
                     /locus_tag="ACHJWA_000568"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HFO4307229.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLKEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(61411..62811)
                     /gene="xanQ"
                     /locus_tag="ACHJWA_000569"
     CDS             complement(61411..62811)
                     /gene="xanQ"
                     /locus_tag="ACHJWA_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HFO4307230.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGALLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(62976..65846)
                     /gene="xdhD"
                     /locus_tag="ACHJWA_000570"
     CDS             complement(62976..65846)
                     /gene="xdhD"
                     /locus_tag="ACHJWA_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFO4307231.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIEVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVDMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(65843..66622)
                     /gene="ygfM"
                     /locus_tag="ACHJWA_000571"
     CDS             complement(65843..66622)
                     /gene="ygfM"
                     /locus_tag="ACHJWA_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFO4307232.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(66672..68000)
                     /gene="ssnA"
                     /locus_tag="ACHJWA_000572"
     CDS             complement(66672..68000)
                     /gene="ssnA"
                     /locus_tag="ACHJWA_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFO4307233.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPSPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVN
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(68003..71101)
                     /gene="ygfK"
                     /locus_tag="ACHJWA_000573"
     CDS             complement(68003..71101)
                     /gene="ygfK"
                     /locus_tag="ACHJWA_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFO4307234.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVHGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQDFHYVLIATGTDKNSGVKLAGDNQNVLKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATVVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLAQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(71423..72001)
                     /gene="mocA"
                     /locus_tag="ACHJWA_000574"
     CDS             complement(71423..72001)
                     /gene="mocA"
                     /locus_tag="ACHJWA_000574"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFO4307235.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIKRPNITNMRQALLMG
                     AHYSVEVENAEIILDIDTPDDFITAQKRYTKI"
     gene            72104..72874
                     /gene="yqeC"
                     /locus_tag="ACHJWA_000575"
     CDS             72104..72874
                     /gene="yqeC"
                     /locus_tag="ACHJWA_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFO4307236.1"
                     /translation="MKSIVDPSALFIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANLGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRAMPLKAPDEHEPCIPKSSCCVIAVMGGHILGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            72922..74547
                     /gene="yqeB"
                     /locus_tag="ACHJWA_000576"
     CDS             72922..74547
                     /gene="yqeB"
                     /locus_tag="ACHJWA_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFO4307237.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIIDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFA
                     AGKDCHAVIETNRGHWLGQVIYCGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(74588..75520)
                     /gene="arcC"
                     /locus_tag="ACHJWA_000577"
     CDS             complement(74588..75520)
                     /gene="arcC"
                     /locus_tag="ACHJWA_000577"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO4307238.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVVTLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVEAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(75568..76953)
                     /gene="hyuA"
                     /locus_tag="ACHJWA_000578"
     CDS             complement(75568..76953)
                     /gene="hyuA"
                     /locus_tag="ACHJWA_000578"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HFO4307239.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(77006..78217)
                     /gene="ygeY"
                     /locus_tag="ACHJWA_000579"
     CDS             complement(77006..78217)
                     /gene="ygeY"
                     /locus_tag="ACHJWA_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFO4307240.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(78275..79471)
                     /gene="dpaL"
                     /locus_tag="ACHJWA_000580"
     CDS             complement(78275..79471)
                     /gene="dpaL"
                     /locus_tag="ACHJWA_000580"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFO4307241.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(79529..80716)
                     /gene="ygeW"
                     /locus_tag="ACHJWA_000581"
     CDS             complement(79529..80716)
                     /gene="ygeW"
                     /locus_tag="ACHJWA_000581"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFO4307242.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEQRTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            81195..82973
                     /gene="ygeV"
                     /locus_tag="ACHJWA_000582"
     CDS             81195..82973
                     /gene="ygeV"
                     /locus_tag="ACHJWA_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFO4307243.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLYRVAG
                     TGAYGKFLGRQLSGNSRLLCHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVTEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(83013..83492)
                     /gene="xdhC"
                     /locus_tag="ACHJWA_000583"
     CDS             complement(83013..83492)
                     /gene="xdhC"
                     /locus_tag="ACHJWA_000583"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFO4307244.1"
                     /translation="MNHSETITIECTINGMPFQLHAVPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(83489..84367)
                     /gene="xdhB"
                     /locus_tag="ACHJWA_000584"
     CDS             complement(83489..84367)
                     /gene="xdhB"
                     /locus_tag="ACHJWA_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFO4307245.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDSITQRHLPALCAAASSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHVGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(84378..86675)
                     /gene="xdhA"
                     /locus_tag="ACHJWA_000585"
     CDS             complement(84378..86675)
                     /gene="xdhA"
                     /locus_tag="ACHJWA_000585"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFO4307246.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWEELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYVSHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNASREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            87091..87846
                     /gene="actS"
                     /locus_tag="ACHJWA_000586"
     CDS             87091..87846
                     /gene="actS"
                     /locus_tag="ACHJWA_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFO4307247.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            87925..87998
                     /locus_tag="ACHJWA_000587"
     tRNA            87925..87998
                     /locus_tag="ACHJWA_000587"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:87957..87959,aa:Gly,seq:ccc)
     gene            88232..88963
                     /gene="ygeQ"
                     /locus_tag="ACHJWA_000588"
                     /pseudo
     CDS             88232..88963
                     /gene="ygeQ"
                     /locus_tag="ACHJWA_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgeQ"
BASE COUNT        21540 a        22160 c        23481 g        21782 t
ORIGIN      
        1 caatcgctgc attaattaaa tgcagaggga acggttatga aaaaatggaa agtacgcagc
       61 gcgttggtgg cattaatcgt gctgctggcc ggttgttcca gtaatgcgca gtataatagc
      121 agtgcatcgg gcaatgtcgg caccgcctgg ggtggggatg tgcactcaac ggttcagggc
      181 gtatctgctg agcgagcctg gcgcgatccg gcggagatga tcgttattag ttattcgact
      241 aatgttcctt ccggctacga tcgcgtttat agcattcgta ttaacgaact ggaatatgct
      301 attcgcgatg gtaattttaa ttctctacct attacgcgtg tttatgattc atctaataat
      361 gaaccacgct atattgtcca tgccagagtc ggaatgaatt atcaattata tgtccgtaat
      421 tacagtcgta acaccaacta cgagatcgtt gctacggttg atggcctgga tgtgctgaac
      481 ggcaagcaag gttcgctgaa taacaacggc tatattgtga atgccggaga ttcgttagcg
      541 attaaaggat tccgcaaaga taaacatacc gaagcggctt ttcagttcgc caacgttgct
      601 gattcctacg cagccaattc ggcgcaaggc gatgtgcgta ataccggtgt aatcggcttt
      661 gccgcctttg aattacaagg ccaggcgcaa aacgcgctgc caccgtgctc tggacaagcc
      721 tttccggcag ataacaatgg ctatgcgccg ccaccgtgtc ggaaatagca acctcgcttg
      781 atgcctcgcc catttaaggc gataattagc acaaacttgt gtttcgcaaa tccaaccctt
      841 cctcgtcggg cctaacgacg cggaagggtt tttttatatc gactttgtaa taggagtcca
      901 tccatgagca ccttaggtca tcaatacgat aactcactgg tttccaacgc ctttggtttt
      961 ttgcgcctgc cgatgaactt ccagccgtat gacagcgatg ctgactgggt gattactggt
     1021 gtgccgttcg atatggccac ttctggtcgt gcggggggac gccacggtcc ggcagctatc
     1081 cgtcaggttt cgacgaatct ggcctgggaa cacaaccgct tcccgtggaa tttcgacatg
     1141 cgtgagcgtc tgaacgtcgt ggactgtggt gatctggtat atgctttcgg cgatgcccgt
     1201 gagatgagcg agaagctgca ggcgcacgcc gagaagctgc tggctgccgg taagcgtatg
     1261 ctctccttcg gtggtgacca ctttgttacg ctgccgctgc tgcgtgctca tgcgaagcat
     1321 ttcggcaaaa tggcgctggt acactttgac gcccacaccg atacctacgc gaacggttgt
     1381 gaatttgacc acggcaccat gttctatacc gcgccgaaag aaggtctgat cgacccgaat
     1441 cattccgtgc agattggtat tcgtaccgaa tttgataaag acaacggctt taccgtgctg
     1501 gacgcctgcc aggtgaacga tcgcagcgtg gatgacgtta tcgcccaggt gaaacaaatt
     1561 gtgggtgata tgccggttta cctgaccttt gatatcgact gcctggatcc tgcatttgcg
     1621 ccgggaaccg gtacgccggt aattggcggt ctgacctccg atcgcgctat taaactggta
     1681 cgcggcctga aagatctcaa catcgttggg atggacgtag tggaagtggc tccggcatac
     1741 gatcagtcgg aaatcaccgc tctggcagcg gcaacgctgg cgctggaaat gctgtatatt
     1801 caggcggcga aaaagggcga gtaagcacca gatgcgatgc gcacgggtaa aacgtgccat
     1861 taatgtcgga tgcggcgtga acgccttatc cgacctacgt tcggcacccg taggccggat
     1921 aagatgcgcc agcatcgcat ccggcaatgc gcacaaggta acaaatgtgc cattcatgtc
     1981 agatgcggcg tgaacgcctt atctgaccta cgttcgacac caccaggctt tacttaatcc
     2041 catccgcgct catgcgatcg cgaatatgct gggcgcgttc ggcggatgca ggatggtcgt
     2101 caaacattga gctttggcga ccttcttcca gttttgccag tttttcaaag ctggtggcaa
     2161 gacctgccgg gctgatgccg cgttggcgca gaagatcgta agaataatca tcggcttctg
     2221 cttcctggcg ctgggagaat tgcgaattaa ctaatttctc gcccagatca ccgagttgtg
     2281 attgagataa actgccgaca atcccgcccg ccgaggccgc agctactcgt acggcatttg
     2341 taccaagtgc cacctgcatt ccttttttca catggcctaa tgccacgtgc cccatttcgt
     2401 gaccgatcac cgcttcgact tcgttatccg tcatcatatc catcagcccg ctatagacac
     2461 ggatacagcc gttagccatt gcaaaggcgt tcacatcctt cgccatatac actttgtaat
     2521 ttaccggctg accgttgata ttgttgccta gcgcattggc aatagttgtc agacgtttag
     2581 cgtattcgct attggctggc gcaatcgtcg ccttgctgtc catctcctga catgcctgat
     2641 cgctcagggt tttcacctgc gcatcactca aactgtaagc ctgaaaagct tccgctcctg
     2701 atgagagcag tccgttggag tccatattct ggcaaccggt cagtaccgtt gccacgctca
     2761 ttgctaccaa taaggcgcga attttcatgt tttttcttcc gcacaaggtc aggattattc
     2821 taaaaatgcg ccgtttgcag gtgaaccgac gctcagtctc agtataagga aaagcgcagc
     2881 ggacgggcga gtagattgcg caacatgcga gcatgatcca gagatttctg aagcggcaaa
     2941 aggatgttcc atgtacatga cgcgcggctt gcggtaaatt gttggcaaat tttccggcgt
     3001 agcccaaaac gcgctgtcgt caagtcgtta agggcgtgcc cttcatcatc cgatctggag
     3061 tcaaaatgtc ctcacgtaaa gagcttgcca atgctattcg tgcgctgagc atggacgcag
     3121 tacagaaagc caaatccggt cacccgggtg cccctatggg tatggctgac attgccgaag
     3181 tcctgtggcg tgatttcctg aaacataacc cgcagaatcc gtcctgggct gaccgtgacc
     3241 gcttcgtgct gtccaacggc cacggctcca tgctgatcta cagcctgctg cacctcaccg
     3301 gttacgatct gccgatggaa gaactgaaaa acttccgtca gctgcactct aaaactccgg
     3361 gccacccgga agtgggttac accgctggtg tggaaaccac caccggtccg ctggggcagg
     3421 gtattgccaa cgcagtaggt atggcgattg cagaaaaaac gctggcggcg cagtttaacc
     3481 gtccgggcca cgacattgtc gaccactaca cctacgcctt catgggcgac ggctgcatga
     3541 tggaaggcat ctcccacgaa gtttgctctc tggcgggtac gctgaagctg ggtaaactga
     3601 ttgcgttcta cgatgacaac ggtatttcta tcgatggtca cgttgaaggc tggttcaccg
     3661 acgacaccgc aatgcgtttc gaagcttacg gctggcacgt tattcgcgac atcgacggtc
     3721 atgacgcagc atccatcaaa cgcgcagtag aagaagcgcg cgcagtgact gacaaaccgt
     3781 ccctgctgat gtgcaaaacc atcatcggtt tcggttcccc gaacaaagcc ggtactcacg
     3841 actcccacgg tgcgccgctg ggtgatgcag aaatcgctct gacccgcgaa cagctgggct
     3901 ggaaatacgc accgttcgaa atcccgtctg aaatctatgc gcagtgggat gcgaaagaag
     3961 caggccaggc gaaagaatct gcatggaatg agaagtttgc ggcttacgcg aaagcttatc
     4021 cgcaggaagc ggctgaattt acccgccgta tgaaaggcga aatgccgtct gacttcgacg
     4081 ctaaagcgaa agagtttatc gctaaactgc aggctaatcc ggcgaaaatc gccagccgta
     4141 aagcgtctca gaatgctatc gaagcgttcg gcccgctgtt gccggaattc ctcggcggct
     4201 ccgctgacct ggcaccgtct aacctgaccc tgtggtctgg ttctaaagca atcaacgaag
     4261 atgctgcggg taactacatc cactacggtg ttcgcgagtt cggtatgacc gcgattgcta
     4321 acggtatctc cctgcacggt ggcttcctgc cgtacacctc caccttcctg atgtttgtcg
     4381 aatacgcacg taacgccgta cgtatggctg cgctgatgaa acagcgtcag gttatggttt
     4441 acacccacga ctccatcggt ctgggcgaag atggcccgac tcaccagccg gttgagcagg
     4501 ttgcttctct gcgcgtgacc ccgaacatgt ctacatggcg tccgtgtgac caggttgaat
     4561 ccgcggtcgc gtggaaatac ggtgttgagc gtcaggacgg tccgaccgcg cttatcctct
     4621 cccgtcagaa cctggcgcag caggaacgta ctgaagagca actggcaaac atcgcgcgcg
     4681 gtggttatgt gctgaaagac tgtgctggtc agccggaact gatcttcatc gcgaccggtt
     4741 cggaagttga actggctgtt gccgcctacg aaaaactgac tgccgaaggc gtgaaggcgc
     4801 gcgtggtttc catgccgtct accgatgcat tcgacaagca ggatgcggct taccgtgaat
     4861 ccgtactgcc gaaagcggtt actgcacgcg ttgcggtaga agcgggtatt gctgactact
     4921 ggtacaagta tgttggcctg aatggcgcta tcatcggtat gaccaccttc ggtgagtcag
     4981 ctccggcaga gttgctgttc gaagagttcg gcttcaccgt cgacaacgtc gttgcgaaag
     5041 caaaagaact gctgtaacta gcatttcggg taaaaaggtc gcttcggcga ccttttttat
     5101 taccttgata tgtccgtttg cggacaagca atagataaag cgtgttgtag atcgcaaata
     5161 tttatatgca ataaatatca attatgtaat atgcatcacg atatgcgtgt tgacatttgt
     5221 tgttatatct ataactcaat gttatataag aaattaataa ttcactgttt tcaaaacacc
     5281 ggtttccctg ctcaattgct ttcattaaac cgcacgagta ttttattttt accgccggac
     5341 ggtaccctaa aaataaaata aggataattt atgcggctta gtgactattt tccagaatca
     5401 tcaatctcag ttatacattc agcaaaagat tggcaggaag ctatcgattt ctcgatggta
     5461 tcattgctgg ataaaaacta tatcagcgag aattacattc aggcaattaa agattccacc
     5521 attaacaatg gcccttatta tattctcgca ccaggcgtgg caatgcctca tgcgcgaccg
     5581 gaatgtgggg cgcttaaaac cgggatgtct ttgacattac ttgaacaagg tgtttatttt
     5641 ccggggaatg acgaaccaat taaattactc atcggactct ctgctgccga tgccgattcg
     5701 cacattggcg ctattcaggc gttaagtgaa ttactgtgcg aagaagaaat actcgaacaa
     5761 ctcttaacag catcatcaga aaaacaatta gcggacatta tcagccgcgg ataattactc
     5821 tctcctctac aggataatat tatggaaaac aagtctgctc gtgcaaaggt ccaggctttt
     5881 gggggctttt tgactgcaat ggtcatcccc aatattggtg cttttattgc ctggggtttt
     5941 attactgcgt tatttattcc caccggttgg ctgcctaacg aacatttcgc caaaattgtc
     6001 ggcccgatga ttacctattt attgcccgtg atgattggtt ctaccggtgg tcatctggtc
     6061 ggcggtaaac gcggcgcggt catgggcgga ataggtacta ttggtgtgat cgtcggcgca
     6121 gagatcccga tgttccttgg ctcaatgatt atggggccgc tcggtggtct ggtcataaaa
     6181 tatgttgata aggcactgga aaaacgcata cctgccggtt ttgagatggt tatcaataac
     6241 ttctcattag gtatcgcggg gatgctcctt tgcctgctgg gttttgaagt gatcggcccg
     6301 gcggtgttaa ttgccaatac tttcgtcaaa gagtgtattg aggcgctggt acatgcgggt
     6361 tatctgcctc tgctgtcagt catcaatgaa ccggcgaaag tgcttttcct taataatgcg
     6421 atcgatcagg gcgtctatta tccgctggga atgcaacagg cttcggttaa cggtaaatcc
     6481 atcttcttta tggtggcctc taacccaggt ccgggcctgg ggctgctgct ggcgtttacc
     6541 ttgtttggta aagggatgag taaacgttct gcgcccgggg cgatgattat tcacttcctc
     6601 ggtgggatcc acgaactgta tttcccatat gtgctgatga agccgctgac tattattgcc
     6661 atgattgcgg gcggtatgtc tggcacctgg atgtttaact tactggacgg tggtctggtg
     6721 gctggcccaa gtccggggtc tatctttgct tacctggcgc tgacgccgaa aggttcgttc
     6781 ctggcgacaa ttgccggtgt tacggtaggt accctggtgt cctttgctat cacttcgctg
     6841 atactgaaga tggaaaaaac ggtggaaacg gagagcgaag atgagtttgc tcagtcagcc
     6901 aatgcggtta aggcgatgaa acaagagggt gcattctcgt taagcagggt taagcgtatc
     6961 gcctttgttt gcgatgcggg gatgggctcc agtgcgatgg gcgcgaccac cttccgtaaa
     7021 cgcctggaaa aagcggggct ggcaattgaa gtaaaacatt acgccataga aaacgtgcct
     7081 gcggatgcgg atatcgtcgt tactcatgcc agtctggaag ggcgcgtgaa acgtgtgacg
     7141 gataaaccac tgatattgat taataactat attggcgatc caaaactcga cactttattt
     7201 aatcaattaa ccgccgaaca taaacactga ttacagaggt aaaaatgaaa accaaagttg
     7261 ctgctattta tggcaagcgg gatgtccgtc tgcgcgaatt tgaactgcca gaaattaccg
     7321 ataatgaatt actggtgagt gtgatttctg acagcgtctg tttatccacc tggaaagcgg
     7381 cgttactcgg tagtgaacat aaacgcgtac ccgacgattt agaaaatcat ccggtcatta
     7441 ccgggcatga atgtgccggg gttattgtcg aagtgggtaa aaatctcact ggcaaatata
     7501 aaaaaggcca gcgttttgta ttgcaaccgg cgatggggtt accaagcgga tattcagcgg
     7561 gctacagcta cgaatatttt ggcggcaatg ccacttatat gattattccc gaaatcgcca
     7621 ttaatttggg ctgcgtatta ccgtatcacg gctcttattt tgctgcggcg tcgctggcag
     7681 agcctatgtg ctgcattatt ggcgcttatc atgccaatta tcacaccacg caatatgttt
     7741 atgagcatcg catgggcgtc aaacctggcg gcaatattgc actgctggcg tgtgcaggtc
     7801 cgatgggcat tggcgctatc gattacgcca ttaacggcgg catacaaccg tcgcgggtgg
     7861 tggtggtcga tatcgacgac aaacgtctgg cgcaggtaca gaagctgctg ccggtggaac
     7921 tggcggccag taaaggcatt gagctggtgt atgtgaatac caaagggatg agcgatcctg
     7981 tccagatgct gcgggcgctg acaggagatg ccgggttcga tgacattttt gtttatgcgg
     8041 cggtgtctgc tgtcgttgag atggctgatg aattactggc ggaagatggc tgtctgaact
     8101 tctttgccgg gccgacggat aaaaacttca aagtgccgtt taatttctac aacgttcatt
     8161 acaacagcac gcacgtcgtc ggtacatccg gcggttcaac ggacgacatg aaagaggcga
     8221 ttgcccttag cgccacaggg cagttacagc cctcgtttat ggtgacccat atcggtggcc
     8281 tggatgcggt gccagaaacc gtgctcaatc tgccggatat ccctggcggt aaaaaactca
     8341 tttataacgg cgtgaccatg ccgctcactg ccattgccga ttttgccgaa aaaggcaaaa
     8401 ccgatccgct gtttaaagag ttggcgcggc tggttgagga aacgcacggc atctggaatg
     8461 aacaggccga gaaatatctg ctggcacaat ttggcgttga tatcggggag gccgcgcaat
     8521 gatgtccctg gcgtggccat tatttcgcgt tacggaacag gcagcgttgg ctgcctggcc
     8581 gcaaaccgga tgtggcgaca aaaataaaat tgatggcctg gcggtcaccg cgatgcgcca
     8641 ggcattaaac gacgtcgctt ttcgtggacg agtggttatc ggcgaaggag aaattgacca
     8701 tgcgccgatg ctgtggattg gcgaagaggt gggcaaaggc gatgggccag aagtcgatat
     8761 cgcggttgat cccattgaag gcacgcggat ggtggcgatg gggcagagca atgcgctggc
     8821 ggtcatggct ttcgcccctc gagatagcct gttacatgcg cccgatatgt atatgaaaaa
     8881 gctggttgtt aatcgactgg ctgccggggc gattgatctg tctttgcctc tggcggacaa
     8941 cttgcgtgac gtggcgcggg cgttaggtaa accgctggac aagctgcgca tggttacgct
     9001 ggataaaccg cgtctgagtg ccgcgattga agaagcaaca cagctgggcg tgaaggtttt
     9061 tgccctgccg gatggcgatg tcgccgccag cgtgctgacc tgctggcagg ataatcctta
     9121 cgatgtgatg tacaccatcg gcggtgcgcc ggaaggcgtg atttctgcct gtgccgttaa
     9181 agcattaggc gggaatatgc aggccgagct gattgacttt tgtcaggcca aaggggatta
     9241 cacggaaaat cggcagattg cagagcagga gcgcaagcgt tgcaaggcaa tgggtgttga
     9301 cgttaaccgt gtgtactcgc tcgatgtact ggtaaggggg aacgatatcc tctttagcgc
     9361 tacgggcgtc acgggcggcg agttggtgaa cggtatccaa cagacggcga atggagtgcg
     9421 gacgcagaca ttactgatcg gcggcgcgga ccaaacgtgt aatataatag actccctgta
     9481 ttgatggtga tctatcggaa gaccaatggc gacgctgtca gaagatgatg tgcttgagca
     9541 actggatgca caggacaatt tattttcatt tatgaaaact gcgcattcta ttttgctcca
     9601 ggggatacgc cagtttctgc cgtcgctgtt tgtcgataac gatgaagaga gcgtcgaata
     9661 tgcagtgaag ccgttactcg cccaaagcgg cccgcttgac gatattgatg ttgcactgcg
     9721 tttgatttat gcgctgggga aaatggataa atggctgtac gccgatatca cgcatttttc
     9781 ccagtactgg cattacctga aagaacagga tgaaacgctc ggatttgccg atgacatgac
     9841 ttgggatttt atcagcaacg tcaatagcat aacctgcaat gcgacgctct atgacgcgtt
     9901 gaaagcgatg aagttcgccg atttcgccgt ctggtcagag gcgcgtttta gcggaatggt
     9961 caaaacggcg ctgacgctgg cagtaacgac caccttaaag gaattaacgc cgtgaaaatt
    10021 gaattaacgg tgaatgggct gaacgtgcaa gcgcagtacc atgatgaaga gatagaacgc
    10081 gtgcataaac cgttgctgcg tatgctggcg gccttacaga cggtaaatcc gcagcgccga
    10141 accgtggttt ttctttgcgc tccgccgggg acgggtaaat ctaccctaac caccttctgg
    10201 gcatatctcg cgcaacaaga cccggagctg cccgctattc agacgctccc gatggatggt
    10261 tttcaccatt acaatagctg gctggatgcg catcaattgc gccccttcaa aggcgcacca
    10321 gagacatttg acgttgcgaa actggcggaa aatctgcgcc aggtcgtgga aggggattgt
    10381 acgtggccgc agtacgatcg acaaaagcat gatcctgttg aagatgcgtt gcacgttacc
    10441 gcaccaataa tcatcgtcga aggaaactgg ctattgctgg atgacgataa ctggcgccag
    10501 ctagcgcaat tttgtgattt ctcgatcttt atcaatgcgc cagctactgc gttgcgggaa
    10561 cgactggtgg ggcgcaagct ggcgggaggg ttatcgctgg cagacgcaga agctttttat
    10621 gaccgtaccg acgggccgaa tgttcgccgg gtgctggaag agagtctgcc tgcaaatttg
    10681 accttaatga tgaccgctac gggtgaatat cgtctggtgg attaatgttc ctgattagac
    10741 catcttccag tcgcaccaca cgagagaaat gacgccgggt aaatgcggcg tcatgactga
    10801 ttgctacgac gctcgtgcct cgctggcggc acttctccag ccagctttca aacacgctca
    10861 accaattttc gtcaaaatcc cgactcggct cgtccagcag caatagtggc ggagagagtg
    10921 cttccaggca ggcgacggcg accatccgtc gctgcgcact atgcagatcc aacgggtgcg
    10981 cgctggcagt gtctgctaac tgacaacatt gcagggcagc atgggtacgt tgagtgattt
    11041 catccgccgg gcatttttgc agtttcaaac caaatgcgat ctcgtcagcg acggtgctgt
    11101 gaaaaagttg gttttccgct tcctgaaaca gtacgccaat ctttgccgcg cgctggcggt
    11161 ttttcaggtt tttcatcgcc tgttgttgca gcataaccgt accggctgtg ggcgtaagaa
    11221 gacctgccat tactcgtaac agcgtcgatt tccctgcgcc attgtcgccg gtcaacgcca
    11281 accattcacc ttgtttgagt tgcagagaaa tgtcgcaaag acaatccgtt gccgcgccag
    11341 gccagcgata agatatctga tttaacgtta acatagtggc tggagcgctc cgttttgcaa
    11401 ttgccacgct tgctggcagt agttcaggaa aggtgtgcga tgacgttcaa aaagaataat
    11461 cagcgaacca cgttcaaacg cccagtgttg taagcgctgc aacagcattt cgctggcctg
    11521 cggcgtcagg cggctaaagg cttcatcaag aattaatagc tttggctgca tcgcgatggc
    11581 gcaggcgatg accacgcgct gtgtctctcc accggagagt gttgccgggt gacgatggcg
    11641 caacggctgg cactcggtca gagtcagggc ggcatcaatc cgcgccataa tctctgcttc
    11701 agcaagacac agattttctg gcccaaaggc aacttcttct tccacgctaa aggtacagcc
    11761 ggagagctgc aaatagggcg attgctggac gagctggatg gtggcggatt gttcattaag
    11821 cgggagatgc ccaatgggcg taccgaggag cgtccccgtg cctgttatct ctcctggcag
    11881 aaagtccgga taccagcccg ccatcagctg tgccagcgta cttttgccac tgccgttgtc
    11941 accaaagatg gcgaccatcc ccggtttgac atagtgaaag tcataacaga acggaggatg
    12001 cgttgagtgt gtcgggcaat aacggaactg ttctaacgta accatatcca cgctccgaat
    12061 tctgtcagca tcagcaagat catggtgtaa cgcgctacgc gttgtagggt gctatcggct
    12121 ggtgcccaca acgttgttcg tcggttgtgg atccgaaatg cccgcatatc cagcgccgca
    12181 ccacggacag caagatcgtt tagcgcattg tgtgttagcg gaatagttaa cgcgggcatc
    12241 gcacgtaaac gctgatacca gccctcatcc agcggcacgc cgcgggcacg ctgcgcttca
    12301 tgaataatcg ctaattgccg ttttaactgc tcaacgacca ataacggccc ggcaaacaga
    12361 taagcgacgc ccggcggcag gcgagaagca aacagagcgc ggataaaaca ttgcacgggg
    12421 acgtactgca tccacaactg tgaggttgaa acaatcgcca gaatccgcaa ccagagcgta
    12481 atagcatgcg tccaacgctc cggcgaacgc ggcgtgccgc ttagccattc tgttagccag
    12541 ccaccgtgta ccagccacag cccggctccc agcgagaaca tcagccagac gacatatttt
    12601 gcccgccgcc gcgtggcctt tagcgcaata aggcaaaaaa aggtcgccgc gctatagatc
    12661 ggcaaaatgg tctgcgcggg caggataagc gtggtgcagg ccgccaatgc ccatagtgtt
    12721 aatgaggtaa atggatgcat ttagcgcacg gcagccattg ccggaaaatg gcgcgtggtg
    12781 cgaatcggca gctggcgcag taaaagccag acaatcaccg ctgtaagagt tttatcgacc
    12841 aggttcgcgc ctatcaccgt aatcgccacc gactccacca ggttttgccc catcgagtgc
    12901 atccaggcaa cgaacaaatc ggctccgcta ccggtgacac cgccgaacag cgcagtacgc
    12961 agcggtacag cgacgaccgt taccgcaagc gtaataatca cgccgctgac aacgactttt
    13021 ggcagggtgc gaaaccaacc cgctcgcgcc agccaaccgg cgaccaggcc gatgaccatc
    13081 gcgacggggg caaaagcggc agcgatcggg tcggtcagta atccccagag taaattggtc
    13141 agtaagccgg tcagcatccc gatcaccgga ccaagcagta ccgcgctaat aagtgtgccg
    13201 atggagtcga gaaatatagg taatttgacc atgctggcca gttgcccgcc gatcatattg
    13261 atggcgatag atataacgat caacaccagt gcctgactgg aaaagtggct acgcgccata
    13321 agaaaacctc tctgaaagta gttatttaac gggggaaata tgcaactcgt cgtaaccaga
    13381 aagcgcgcaa ggttggcggc taaacgccag ttcctcaagg ctaccgatca ccagttcaag
    13441 cgtggtccgc acggtacagc cgggcatcat atgaccgcca agcatcgtgc cgtgtggatc
    13501 ggaaacgcag agatgaagat gctcaccact ctgttccaac gtgccgttta gtgagatcac
    13561 ttcgaatgta ccgttcagga gcgtggtgcc ttcttgcccg gcatagcgca aggcgatgtc
    13621 agtcaggcta ccggtacagc ctgcaatcca ggcggcatga agttgctgct gctgtgcaaa
    13681 agcgtgtagc tgggaaaaaa cttcctgccc ggggaggagg cgcagtgcgt aaaagcgtgc
    13741 ggaggaagaa tatgggtagg gcaatgtcat cattggagta ctccgaaact cggacgtatt
    13801 acggataata tcgacgctgc atcagtcata ttctgatata agtcagggtt tgtgacggca
    13861 caacgaaaag attttgctac gcttattcgt gattctgcaa agtggaataa attccacagg
    13921 ggcggggctg aaaacgtgac gtaagtcaga taattgtttt ctttgactgg acaatcattc
    13981 cttttattcc acgtttcgct tatcctagct gaagcgtttc agtcgattaa atgttcgaca
    14041 attaaccaat cagtcgcagt ttgcgacagg taaggtttcc ccggacgatt tgctggatta
    14101 ctctgtctgg caaatttgct cagggaaaac cttgcaggag atctatgacc gtacgcgtag
    14161 cgataaatgg cttcggtcgc atcgggcgta atgtggttcg tgctttgtat gaatccggac
    14221 gccgggcgga aattaccgtg gtggcaatca acgaactggc ggatgctgcg ggcatggcgc
    14281 atttgttgaa atacgacacc agccatggcc gttttgcatg ggaagtacga caggaacgcg
    14341 atcaactttt tgttggtgat gacgccatcc gcgtattgca tgaacgttca ctgcaatcgc
    14401 tcccctggcg tgaacttggc gttgatgtag tcctcgactg caccggcgta tatggctccc
    14461 gcgagcatgg cgaagcgcac attgccgccg gggctaaaaa agtgctcttt tcacatcctg
    14521 gcagtaacga tctcgacgcg accgttgttt acggcgtcaa tcaggatcaa cttcgtgcgg
    14581 agcaccgcat cgtttctaac gcttcctgta ccacgaattg cataattccc gtcatcaaat
    14641 tgttagatga tgcgtacggt attgagtccg gcactgtgac cacaattcac tccgccatgc
    14701 acgatcaaca ggttattgat gcataccatc ctgacctgcg tcgcacccgg gcagccagcc
    14761 agtcgatcat tccggtcgat actaaactgg ccgccggtat cacacgattt tttccgcaat
    14821 ttaacgatcg ctttgaagcg attgcggtac gtgtgccaac cataaatgtg acggcaatcg
    14881 atttaagcgt gacggtgaag aaacctgtaa aagccaatga agtcaacctg ttgctgcaaa
    14941 aagcagcaca aggtgcattt catggtatag ttgactatac ggaattgccg ttggtctctg
    15001 tagattttaa ccacgatccg cacagtgcca ttgtcgatgg cacccaaacc cgggtcagtg
    15061 gcgcacacct gatcaaaacg ttggtctggt gcgataacga atggggcttt gctaaccgaa
    15121 tgctcgacac gacgttagct atggctactg ttgctttcag gtaagacgca agcagcgtct
    15181 gcaaaacttt tagaatcaac gagaggattc accatgtctg taattaagat gaccgatctg
    15241 gatcttgctg ggaaacgtgt atttatccgt gcggatctga acgtaccagt aaaagacggg
    15301 aaagtaacca gcgacgcgcg tatccgtgct tctctgccga ccatcgaact ggccctgaaa
    15361 caaggcgcaa aagtgatggt aacttcccac ctgggtcgtc ctaccgaagg cgagtacaac
    15421 gaagaattct ctctgctgcc ggttgttaac tacctgaaag acaaactgtc taacccggtt
    15481 cgtctggtta aagattacct cgacggcgtt gacgttgctg aaggtgaact ggttgttctg
    15541 gaaaacgttc gcttcaacaa aggcgagaag aaagacgacg aaatcctgtc caaaaaatac
    15601 gctgcactgt gtgacgtgtt cgtaatggac gcattcggta ctgctcaccg cgcgcaggct
    15661 tctactcacg gtatcggtaa attcgctgac gttgcgtgcg caggcccgct gctggcagct
    15721 gaactggacg cgctgggtaa ggcactgaaa gaacctgctc gcccgatggt ggctatcgtt
    15781 ggtggttcta aagtatctac caaactgacc gttctggact ccctgtctaa aatcgctgac
    15841 cagctgattg ttggtggtgg tatcgctaac acctttatcg cggcacaagg ccacgatgtg
    15901 ggtaaatccc tgtacgaagc tgacctggtt gacgaagcaa aacgtctgct gaccacctgc
    15961 aacatcccgg ttccgtctga tgttcgcgta gcaaccgagt tctctgaaac cgctccggct
    16021 accctgaaat ctgttaacga cgtgaaagct gacgagcaga tcctggatat cggtgatgct
    16081 tccgctcagg aactggctga aatcctgaag aatgcgaaaa ccattctgtg gaacggtccg
    16141 gttggcgtgt tcgaattccc gaacttccgc aaaggtactg aaatcgtggc taacgctatc
    16201 gcagacagcg aagcgttctc catcgctggc ggcggcgaca ctctggcagc aatcgacctg
    16261 ttcggcattg ctgacaaaat ctcctacatc tccactggcg gcggcgcatt cctcgaattc
    16321 gtggaaggta aagtactgcc tgcagtagcg atgctcgaag agcgcgctaa gaagtaaaaa
    16381 atcacagggc agggaaacct gcccttgttt cagcgcgctt ttagagcacg cattttttca
    16441 tactctaaat aattcgagtt gcaggaaggc gacaagagag tgaatcccca ggagcttaca
    16501 taagtaagtg actggggtga gcgaacgcag acgcagcaca tgcaacttga agtatgacga
    16561 gtataaggcc cgacgataca ggacaagaga catgtctaag atttttgatt tcgtaaaacc
    16621 tggcgtaatc actggtgatg acgtacagaa agttttccag gtagcaaaag aaaacaactt
    16681 cgcactgcca gcagtaaact gcgtcggtac tgactccatc aacgccgtac tggaaactgc
    16741 tgctaaagtt aaagcgccgg ttatcgttca gttctccaac ggtggtgctt cctttatcgc
    16801 tggtaaaggc gtgaaatctg acgttccgca gggtgctgct atcctgggcg cgatctctgg
    16861 tgcgcatcac gttcaccaga tggctgaaca ttatggtgtt ccggttatcc tgcacactga
    16921 ccactgcgcg aagaaactgc tgccgtggat cgacggtctg ctggacgcgg gtgaaaaaca
    16981 cttcgcagct accggtaagc cgctgttctc ttctcacatg atcgacctgt ctgaagaatc
    17041 tctgcaagag aacatcgaaa tctgctctaa atacctggag cgcatgtcca aaatcggcat
    17101 gactctggaa atcgaactgg gttgcaccgg tggtgaagaa gacggcgtgg acaacagcca
    17161 catggacgct tctgcactgt acacccagcc ggaagacgtt gattacgcat acaccgaact
    17221 gagcaaaatc agcccgcgtt tcaccatcgc agcgtccttc ggtaacgtac acggtgttta
    17281 caagccgggt aacgtggttc tgactccgac catcctgcgt gattctcagg aatatgtttc
    17341 taagaaacac aacctgccgc acaacagcct gaacttcgta ttccacggtg gttccggttc
    17401 tactgctcag gaaatcaaag actccgtaag ctacggcgta gtgaaaatga acatcgatac
    17461 cgatacccaa tgggcaacct gggaaggcgt tctgaactac tacaaagcga acgaagctta
    17521 tctgcagggt cagctgggta acccgaaagg cgaagatcag ccgaacaaga aatactacga
    17581 tccgcgcgta tggctgcgtg ccggtcagac ttcgatgatc gctcgtctgg aaaaagcatt
    17641 ccaggaactg aacgcgatcg acgttctgta agtcattcct ttctgcttat ctcaaggccc
    17701 gctctgcggg tctttttttc gccaaaagca aaattgcctg atgcgctatg cttatcaggc
    17761 ctacacgtat cctgcaatat attgaatttg caggtttttg taggccggat aaggcgttca
    17821 cgccgcatcc ggcatgacaa agcgcacatt gatatctgtc tgagccccgc tctgcgggga
    17881 tttttttgcc aaaagcaagg tgattcagat gagaaatctg tgatctattt ggcaaaatta
    17941 tgctttattg tttacccttg tcagactgcc cgtcataagg cggcggagtg tatttctcca
    18001 ttttgagtca gttgaaaagg aatattgaat ggaagatttg aatgttgtcg atagcataaa
    18061 cggcgcggga agctggctgg tagctaacca ggcgctgctg ctaagttatg cagtaaacat
    18121 cgtggcggca ctcgcgatca tcatcgttgg tttgattatc gcgcggatga tttccaacgc
    18181 ggtgaatcgc ctgatgatct cccgtaaaat cgatgccact gttgctgatt ttctttctgc
    18241 attagtccgt tacggtatta tcgcctttac gctaatcgca gccctgggac gcgtgggtgt
    18301 acaaacagcg tcagtcattg ctgtactcgg tgccgcaggc ttagctgttg gtctggcttt
    18361 gcaggggtca ctttctaacc tggccgctgg cgtgttactt gtcatgttcc gcccgttccg
    18421 tgccggagaa tatgttgacc tcggcggcgt agccggtact gtgctgagtg tgcagatttt
    18481 ctccaccacc atgcgtactg cagacggtaa aattatcgtt attccgaacg gtaaaattat
    18541 tgccggaaat attattaact tctcccgcga gccagttcgc cgtaacgaat ttattattgg
    18601 cgtggcgtat gattccgata tcgatcaggt taagcagatc ctgaccaata ttatccagtc
    18661 tgaagatcgc attttgaaag atcgcgaaat gactgtgcgc ctgaacgaac ttggtgcatc
    18721 gtcgattaat ttcgtggtcc gcgtctggag caacagcggc gatctgcaaa acgtgtactg
    18781 ggatgtgctg gagcgtatta aacgtgaatt tgatgccgcc ggtatcagct tcccgtaccc
    18841 gcaaatggat gtgaacttta agcgggtgaa agaagacaaa gctgcgtaat caacgctgag
    18901 gccagataat actctctggc ctctctctta ttagttattc tgattgccaa ttaatattat
    18961 caatttccgc taataacaat cccgcgatat agtctctgca tcagatactt aattcggaat
    19021 atccaacgtg ttttcttatt actttcaagg tcttgctctt ggggcagcta tgattctgcc
    19081 gctcggccca caaaatgctt ttgtgatgaa tcagggcatt cgtcgtcagt accacattat
    19141 gattgcctta ctttgcgcta tcagcgattt ggtcctgatt tgcgcgggga tttttggtgg
    19201 tagcgcgtta ttgatgcagt cgccgtggtt gctggcgctg gtcacctggg gcggcgtagt
    19261 cttcttgctg tggtatggtt ttggcgcttt taaaacagcg atgagtagta atattgagtt
    19321 agctagtgcc gaagtcctga agcaaggcag atggaaaatt atcgccacca tgttggcagt
    19381 gacctggctg aatccgcatg tttacctaga tacttttgtt gtactgggca gccttggcgg
    19441 gcagcttgac gtcgaaccaa aacgttggtt tgcgctcggg acaattagcg cctctttcct
    19501 gtggttcttt ggtctggcta ttctcgcagc ctggctggca ccgcgcctgc gcacggcaaa
    19561 atcacagcgc attatcaatc tggtagtggg atgtgtcatg tggtttatag ccttgcagct
    19621 ggcgagagac ggtattgctc atgcacaagc cttgttcagt taggcgctat ctgatggaaa
    19681 aataaaacag aggcgctaag cttgcctcca gagatcctga attttgggca agtcaattaa
    19741 cacggagaga ctaacgtgaa gttcaaagtt atcgccctgg cggcattaat gggtattagc
    19801 gggatggcag cgcaggctaa cgaattgccg gatggaccgc atattgtcac ctccggtacg
    19861 gcaagtgtgg atgcggtgcc agacattgcc actcttgcga ttgaagttaa cgtggccgcg
    19921 aaggatgccg ctacagccaa gaaacaggca gatgagcgcg tcgcacaata catttccttc
    19981 cttgaactca atcagatcgc gaaaaaagat atcagctcag cgaatttacg cacccagcca
    20041 gattatgatt atcaggatgg taaaagtatc ctgaaaggct accgcgctgt gagaacggtg
    20101 gaagtcacgc tccgtcagtt agacaaactg aattccttgc tggatggcgc gctgaaggcg
    20161 ggtcttaacg aaattcgttc agtgtcgctg ggcgtggcgc agccggatgc ctataaagac
    20221 aaagcgcgta aggcagcgat tgataacgcg attcatcagg cgcaggaact ggcgaacggc
    20281 tttcatcgta aactggggcc ggtatatagc gtgcgctacc atgtttccaa ctatcagccc
    20341 agcccaatgg tgcggatgat gaaagccgat gccgcgccgg tgtctgccca ggaaacttac
    20401 gagcaggccg ctattcagtt tgatgatcag gtcgatgtgg tcttccagtt agaacctgtg
    20461 gatcaacaac ccgctaaaac acctgcagca caataatact tgtagcccga cgtattcgtc
    20521 gggctaatgc ctttacccga tattgcctcc tttttctgta attttctgta aaaccatgac
    20581 gtaatcacct atctttgctc gcggtttcat caaggatagg ctgactgaaa cggttaagcg
    20641 gacaaggatg cggggagggg ggatggacat ttttatttcg aaaaagatgc gcaatttcat
    20701 tttattagcg caaaccaata atattgcccg ggcggcagaa aaaatccata tgacggcttc
    20761 gccatttggt aaaagcattg ccgcgctgga agaacaaatt ggctatacgc tatttacccg
    20821 caaagataat aatatcagcc tcaataaagc cggtcaggaa ctgtatcaaa aactgttccc
    20881 ggtttatcaa cgactttctg cgattgataa tgaaatccat aactcagggc gtcgttcacg
    20941 agaaattgtg attggcatag ataatactta tccgacaatt attttcgatc agttgattag
    21001 tctcggagat aagtatgaag gcgtgaccgc ccagccagtt gagttcagcg aaaacggcgt
    21061 tatcgataat ctttttgacc gtcagttaga ttttattatc tccccacagc atgtatctgc
    21121 acgagttcag gaactggaaa acctgactat cagtgaatta ccaccgctgc gattgaactt
    21181 tctcgtttct cgccgttatg aagagcgtca agagcaagag ctgcttcagg agctgcccta
    21241 gctacaaatg cgctttcaaa accgggctaa cttcgaagca atgattgatg cgaatatgcg
    21301 accttgtggg atcaatccca ccattattta tcgtccctac agttttatgg ccaaaatcag
    21361 cgccgtggag cgcggacatt ttctgacggt gatcccgcat tttgcctggc gactggtgaa
    21421 tccggcaacg ctgaaatatt ttgatgcgcc gcacaggccg atgtatatgc aggaatatct
    21481 ttattcaatc agaaatcatc gttatactgc cacgatgctt cagcatattg ctgaagatcg
    21541 tgacgggaca aaccattaac ccagcatcga gccggttgca attaaattac ggtgtaagtc
    21601 gaagacgtgg ctaagatcgt ggtgaatatg tccgccgggc gcattttcca gatagcgatg
    21661 cagataatcc cgatacatag gatgtgcaca attatcaatg atagtgcggg cgcgttgaag
    21721 cggggaaaga ccgcgcagat cggcgatccc ttgttcggtg atgatcactt tgacgctgtg
    21781 ttcgttgtga tcaacatggc tgcacattgg cacgacggtt gagatcttgc cttctttagc
    21841 aatcgacgga gccataaaga tcgacagata cgcgttgcgt tcaaaatcac cgctgccgcc
    21901 gatgccgttc atcagatcga ccccggctac gtgcgttgag ttggcatgcc cgtaaatatc
    21961 aaactccagg ccgacgttca gagcgatgac gcccagacga cggatgattt ccgggttatt
    22021 ggaaatctcc tgcggacgca acacaatgcg gctggcaaag taatccatat tgtcgtaaat
    22081 cttgcgcagg gaatcggccg agattgtcag gctggaggcg ctggccccgc tgattttgcc
    22141 ggtttccagt aaatgcacca ccgattcctg tagcacttcc gaatacatca taaacggagg
    22201 aatttccggg ttttccccca gacgcgccat taccgcatta ttgatattgc ccacgccact
    22261 ttgcagcggc agaaattccg gcggaatacg cccatgcgcc atttcctgca ataagaacgt
    22321 gaccacgtta tcggcaatct gctggcacat gggattttgc ttatccagca tattaccggc
    22381 gtcgggcaag ttggtttcca cgacggcgac aatctttttc ggatcgattt gcacatagcg
    22441 ggtaccgacg cgatccattg catggaagat cgacacgcta ttgcgccgcg gtggcgcgcc
    22501 aggaatcaca atatccgcca gttctgcaac gcgcggatcg tgatagtgat tgagttcaat
    22561 gatcactttc ttcgctcgca gcagccaggt cggcgcatta ccgatcccgc tggttaacca
    22621 gactcgacca tctggtgcca gtgccgatgc ttcaatgacg gcaacatcaa tgtcgccgaa
    22681 gaaaccgtaa ttgaccattt gcgccacttc gctcaaatgc aggtcaacga aactcaccgc
    22741 gccctgattg atctttttac gtaaaccgga cgatgtttga tatggcgcac gccaggaaac
    22801 agcatcggcg tcagaaagta catcgtcagc ggcggcgctg attgacgccc ccgtcagcag
    22861 gcgaatttga tacggctttt tggcctcatg ctgttcgtta gctctgcggg caatcgcggt
    22921 gggtagggct ttcggcgaac ccgctggggt aaagccgcta aatgccacca tgtcgttatg
    22981 ctggataatt tctgccgctt catcggcggt catccttgtc cactgagttt ccatttttac
    23041 tccttcgttc gcagggatta atgaccaacg aaattaggtt tacgtttttc gaggaaagcg
    23101 ttcatccctt cctggtaatc ttcgctgtca taaaccgcgc ggcgcatccc ctgaatacgt
    23161 tcaaattcat cggagttcat ggtgtgtgct tcgcccagta cacgcagctc ttctttgata
    23221 acggcaatgg ctaacggcgc tttctcagag atgtggtgcg ccatttgtaa ggtgaaatct
    23281 tccagttctt ccacttccac aacatggttg aggatgccga cagccagcgc gcgctgggcg
    23341 gtgatgggcg aagcggtaaa aatcagctct ttgacaatgt ggaagcccgc gtcgcgggtc
    23401 aggttgtgaa tgccgaccag gttatacggg acgccgaggt ttacaggcgt cattgagaag
    23461 gttgaggtac tggcggcgat aatcagatcg gaactcatga tcatttcaaa tgcgccaccc
    23521 caaacactac cttccaccat cgaaataatc ggtttcggga atttttggat catgcgggtg
    23581 atttgacgca atggatcatc ataggagagc ggatcgcgac cgccagacgg cagttcgtga
    23641 atatcgtgac ctgcggagaa gactttggat ccactcggcg cgcgcaaaat gatacagcga
    23701 atttccggcc ggttgagatc gcttaacgcc tgcataagat catcaataaa gactttactt
    23761 aaggcattaa gttttcggcc atagttaaac tcaatgaccg ccactttgtt gatagtgaca
    23821 acgttaacat actgataaga cataaaaatt cctttaatca aaatattgcg tctggataaa
    23881 ttcactgagc tgccgcaggc cggtgcgcgg tgagagcgta ttgtttttga ccgctaaaag
    23941 cgtctggcgg taatagcgat cgaaatcttc attcgcgaac aggtgattca gtacttcttc
    24001 ttcggtctgc ttacgcagcc attccaccga ttgttgttgc cgcacttgtt gtaaacgacc
    24061 actggcagtt agcgcggttt tgaagtcgat gatggcgtgc cagatctcat cgattccacg
    24121 tttttccagt gcgctacaag tcagaacccg tggctgccat tcgtcgtatt tacgtcgcag
    24181 aatatgcagg gcactctcgt acatatgccg ggcaatggcg acattggtat ggttatcgcc
    24241 atcgtctttg ttgataacga tcagatcagc cacttccatc agcccttttt taatgccctg
    24301 cagatcatcg ccgccaccgg caatttgcaa cgagataaaa cagtccacca tgcgggcgac
    24361 ttctgtttcc gactgcccga cgccaaccgt ttcgacaatc actacgtcat aacccgctgc
    24421 ttcgcataac agcattaatt cccgcgctcg ctgactggca ccgcccagat gaccggagga
    24481 tggtaccggg cgaataaacg ccgcttcggc acgcgccagg tcattcatgc gggttttatc
    24541 cccgagaatg ctaccgccag tgaccgggct gctgggatcg accgcaataa ccgcgacctt
    24601 taatccctct cgaatcaaca acatgccaaa ggcctcaaga aaggtacttt tccccgcgcc
    24661 gggggtgccg gtaacgccca gtcgcagggt gttaccgcag tacggcataa tggcatcaag
    24721 cagctgcgta cttagtgcct gatgacgcgg gtgacggctt tccaccagcg tcatggcctg
    24781 ggcgagtgtg gcatgctcac cctgacgtaa gcggcgaata ctttctgcca gcgtggcttc
    24841 attaatcatg atgctggctt atcagattca gtacgtcgcg cacactgtcg agcataggtg
    24901 tacctggacc ataaatcgcc gccacgccgc gctcttgcag gaaggcgtaa tcctgcggtg
    24961 gaatgacgcc accggcgact acgcagatat cttcgcgtcc ccattttttc agcgcttcga
    25021 ccagttccgg gatcagcgtt ttatgaccgg cagccagtga ggatgcgccc actacgtgaa
    25081 cgtcattttc tactgccagg cgggcgatct cttcaggtgt agagaacatc gggcttaaat
    25141 ctacgtcgaa accgagatcg gaataggcgc tggcgatcac tttcgcgccg cgatcgtgtc
    25201 catccaggcc cattttggcg atcagaatgc gcgggcgacg accattgtcg gcaaggaact
    25261 gctccgtttg cgcaacaatg gcatcgaact cggaggccga tttctcagat tgatgatagc
    25321 tttgcgcaat cacgccggta acacactggc ttggcaccag ataacggtcg aaagcggctt
    25381 ccagcgcatc ggaaatttca cccagggtgg cgcgaacgcg agcggcatta acagcggcag
    25441 ccagcaggtt ttcgttatgc tgtgcggcgt gagtcagggc gttcaacgcg gcggttacgg
    25501 cggcatcatc acgggtggcg cgaatgcgtt ccagcgaagc aatttgctcg ttacgcacca
    25561 tcacgttgtc gatctcaagt acatcggttt cgtcttcgtg atccagtttg tacttgttga
    25621 caccaacgat gacacgcttg ccctggtcga tcagcgactg ttcgcgcgct gaggcctctt
    25681 cgatcattcg ttttggcaga cctgcttcga tcgctttcgc catgccaccg gcttcgtcga
    25741 tctgttggat aatagctctg gcttgtttga cgatttgatc ggtcagcgac tcaatgtaat
    25801 aggatccggc cagtggatcg acggtgcggc agagttctga ttcttcctgg atgatgatct
    25861 gggtgttgcg ggcaatgcgt gctgagaaat cggtaggcaa accaagcgct tcgtcaaagg
    25921 cgttggtatg cagtgactga gtaccgccca gcgtcgcagc cagcgcttca atggtggtgc
    25981 ggataacgtt gttatacgga tcctgttcag tcaggctcca gcctgaggtc tggcagtggg
    26041 tacgcagcgc cagtgatttc gggtcctgtg cgccaaatcc actgaccgct tcgctccata
    26101 aataacgtgc cgcacgcaac atggcgacgt tcataaacag atccatgccg atgccgaaga
    26161 agaacgacag gcgaggagcg aagtcatcaa ttttcagtcc ggcagagatt gctgctttga
    26221 tgtactcaat cccatcagcg agcgtaaatg ctacctgctg cacgcagttg gcacccgctt
    26281 cacccatgtg gtaaccgctg atactgatgg tattaaatcg cggcatgttg ccggaacacc
    26341 aggcgatgat gtcggcgata atgcgcattg acggttttgg cgggtaaata taggtgttgc
    26401 ggcagaggta ctctttgaga atatcgtttt gaatggtgcc ggtcagttta tcaggtgtaa
    26461 caccttgctc ttccgcggcg atgatataaa acgccagtac tggtagcact gcgccattca
    26521 tggtcatcga aaccgacatt ttatccagcg ggatctggtc gaacaggact ttcatatctt
    26581 ccacggtgtc gatagcgacg cccgttttgc cgacgtcacc cgccacgcgt gggttatcgg
    26641 agtcgtagcc acggtgggtg gcaaggtcaa acgcaacgga aagacctttt tgcccggcgg
    26701 ccaggttacg gcgataaaaa gcgttggact cttttgctgt tgaaaaacca gcatactgac
    26761 ggatggtcca cggttgggcg gtatacatag tggcacgcgg gccacgaacg tagggcggca
    26821 aaccaggaag ggtacctgtc acctccagat tatcgagatc ggcttcggta tacagcggct
    26881 tgatggcgat cccttccgcg gtttgctgaa ccagcgagtc gacagttttc tcccgacggc
    26941 tcaattcctt gttggcaagc tgttgccact cctgctcgtt agacattatt tgctccgtaa
    27001 aacgcaatca aaatgagggt atttgcgctt acggtgagct ttttacgtgg gtttgtcgcc
    27061 agcaaaaaaa atgcattcag acgtcgtttt ttgccgcctg agatttgtct attttgtgat
    27121 gtgattcaag gaatacaaaa agaagtgagg ataatgcctg atatgtgcta tcaggcatta
    27181 tttgatggat taatcctgac gaaggacttt gtgaccataa tcgagcaacg catcagtgac
    27241 tttacgcatc atgcggcttt caggagcaaa gcggtgccag tagagcatcc ggcgttgaaa
    27301 tagccctggc gttaagtcaa tcagttcacc gctggctagt tctttctcga tttgcaggtg
    27361 cgggatcata cagcaggtgg tgccctggcg agcaagttgc acgaacgctt ctgaagaatt
    27421 aacgatatgg cagggcacgc tgcctggagg cagatcgaag ttttgctgca aaaaggcctg
    27481 gtgcatatcg tcaagatggt caaacgcgac cactggcgct ttcagtaatg ccgaacgcgt
    27541 tacgccgtta gggaaatatt tttcggcaaa gggttttgag ctgacgaaca gatagtcgag
    27601 cgcaccaagt ttatcgacaa gacaactcgg cagcgcctga tgttgaatac tcaccgcgcc
    27661 gaccacttcg ccgcgacgca gacgttcctg agtgcgggtt tcatcttcta cctgcaagtt
    27721 gaggcggata ggcgaatcag ccaatacagg agccagtgcc ggaagcaacc acgtcgccag
    27781 actgtcggca ttgaccgcca gtgaaagcag cagcggagtc gaaccggttt gttcatcgcc
    27841 cagccactct tcttccagca actccacctg gcgcagcagt gccagcagtt tttgcccttg
    27901 ttccgtcggg cgcggcggta cggtacgcac caacagcggc tgcccgaaca tattttccag
    27961 ttgcttaatg cgctgtgaga cggctgattg tgtaatgcac agcttttgtg ccgcgcgctc
    28021 aaatcctcgt tcacgtatca ccgcatccag tgcctgtaat gttctgtagt ccgggcgttt
    28081 cattgctgtt gtctgctcct ggtaaatgct ttttcctgca ctatgacaca attttgtgtc
    28141 aggttgcata taccgcagcc gtcggcgggt aacatttgta tcaatggtta aacgcgcagc
    28201 ttaaaagatc agggatttgc gctattgcat ttcgcttagc aacttcgcgc cctggttatg
    28261 ttgaatggcg tggcgttatt gcctctgttc gcaccgtgga gcggcatacc cagaccgttg
    28321 tatgctttat ttgcggggcg aaaggggatg cccgccattg cgcgatattt tatggatgag
    28381 ttaaccacgc ggcttgccaa cggggtctga atcgcttttt ttgtatataa tgcgtgtgaa
    28441 atttcatacc acaggcgaaa cgatcatgac gcaggatgaa ttgaaaaaag cagtaggatg
    28501 ggcggcactt cagtatgttc agcccggcac cattgttggt gtaggtacag gttccaccgc
    28561 cgcacacttt attgacgcgc tcggtacaat gaaaggccag attgaagggg ccgtttccag
    28621 ttcagatgct tccactgaaa aactgaaaag cctcggcatt cacgtttttg atctcaacga
    28681 agtcgacagc cttggcatct acgttgatgg cgcagatgaa atcaacggcc acatgcaaat
    28741 gatcaaaggc ggcggcgcgg cgctgacccg tgaaaaaatc attgcttcgg ttgcagaaaa
    28801 atttatctgt attgcagacg cttccaagca ggttgatatt ctgggtaaat tcccgctgcc
    28861 agtagaagtt atcccgatgg cacgtagtgc agtggcgcgc cagctggtga aactgggcgg
    28921 tcgtccggaa taccgtcagg gcgtagtgac cgataacggc aacgtgatcc tcgacgtcca
    28981 cggcatggaa atccttgacc cgatagcgat ggaaaacgcc ataaatgcga ttcctggcgt
    29041 ggtgactgtt ggcttgtttg ctaaccgtgg cgcggacgtt gcgctgattg gcacacctga
    29101 cggtgtcaaa accattgtga aatgatctga cgggggaacc tcccccgtta aaaaaattct
    29161 cttcattaaa tttggtgaca tgtgtcacgt ttttacctgg atattgtcga ttgctctaaa
    29221 taaatcctct aaaccagcat attcaaccaa gaaatacctt tgcgtgatat ttcctcaaca
    29281 tcgcgacgca aacgttcata ttgccgcaat attatttttt gatatgttga aaggcggatg
    29341 caaatccgca cacaacattt caaagacagg attgggtaaa tggcaaaggt atcgctggag
    29401 aaagacaaga ttaagtttct gctggtagaa ggcgtgcacc aaaaggcgct ggaaagcctt
    29461 cgtgcagctg gttacaccaa catcgaattt cacaaaggcg cgctggatga tgaacaatta
    29521 aaagaatcca tccgcgatgc ccacttcatc ggcctgcgat cccgtaccca tctgactgaa
    29581 gacgtgatca acgccgcaga aaagctggtc gctattggct gtttctgtat aggaacaaac
    29641 caggttgatc tggatgcggc ggcaaagcgc gggatcccgg tatttaacgc accgttctca
    29701 aatacgcgct ctgttgcgga gctggtgatt ggcgaactgc tgctgctatt gcgcggcgtg
    29761 ccggaagcca atgctaaagc gcaccgtggc gtgtggaaca aactggcggc gggttctttt
    29821 gaagcgcgcg gcaaaaagct gggtatcatc ggctacggtc atattggtac gcaattgggc
    29881 attctggctg aatcgctggg aatgtatgtt tacttttatg atattgaaaa caaactgccg
    29941 ctgggcaacg ccactcaggt acagcatctt tctgacctgc tgaatatgag cgatgtggtg
    30001 agtctgcatg taccagagaa tccgtccacc aaaaatatga tgggcgcgaa agagatttcg
    30061 ctaatgaagc ccggctcgct gctgattaat gcctcgcgcg gtactgtggt ggatattccg
    30121 gcgctgtgtg atgcgctggc gagcaaacat ctggcggggg cggcaatcga cgtattcccg
    30181 acggaaccgg cgaccaatag cgatccattt acctctccac tgtgtgaatt cgacaacgtc
    30241 cttctgacgc cacacattgg cggttcgact caggaagcgc aggagaatat cggcctggaa
    30301 gttgcgggta aattgatcaa gtattctgac aatggctcaa cgctctctgc ggtgaacttc
    30361 ccggaagtct cgctgccact gcatggtggt cgtcgtctga tgcacatcca cgaaaaccgt
    30421 ccgggcgtgc taactgcgct gaacaaaatc ttcgccgagc agggcgtcaa catcgccgcg
    30481 caatatctac aaacttccgc ccagatgggt tatgtagtta ttgatattga agctgacgaa
    30541 gacgttgccg aaaaagcgct gcaggcaatg aaagctattc cgggtaccat tcgcgcccgt
    30601 ctgctgtact aattcccctt ctctgaaaat taacgggcag gtcactgact tgcccgtttt
    30661 tttatccctt ctccacaccg ttgccgcgtt gcgcattcta ctttgcgagt ctgctccaga
    30721 aaataaatca aagtcagatc aatgcgttgt gtttatttaa tggggattta cgatggcggg
    30781 acagcatggg gctgtaacgg tgaagccctc accgaagcga gggcttaaag gagaagggtt
    30841 atgatgcgac ttgtcatcat actgattgta ctgttactga taagtttcgg agcttattaa
    30901 cagtcagtct caggggagga gtcatcctcc cttaccctta ctcactaaat taggtcaaag
    30961 aatcaacgat gtcaatcagg gcgatgcggg gtgcatcgcc ctgaaccgaa ctttttgata
    31021 gccacttcat cgcccacgtt tactgccgta attaatccct tattcctgca atgtatgtct
    31081 gccttacatg acgttttacg agtgcgctcg aagaaaagat ttaaaggtag atcaatgcgt
    31141 tgtggttatt taaagagagt ttacgatggc agggcagcat ggggctgtaa cggtaaagcc
    31201 ctcaccgaag cgagggcttg aaggagaagg gttatgatgc gacttgtcat catactgatt
    31261 gtactgttac tgataagttt gccagcttat taacagtcag tctcagggga ggagtcatcc
    31321 tcccttaccc ttactcacta aattaggtta aagaatcaac gatgtcaatc agggcgatgc
    31381 aggtgtatcg cccttaccac tcccagactt tcgacggtgt aaccaccgca ggaagaggga
    31441 tatcccactc ttcaacgggg agtttttcca ccaactgaca atcatgcgca taacccaccg
    31501 gttgcgtttt atagtgctgc cagttttgta aggtccgatc ataaaaacca ccgcccattc
    31561 ccaggcgctg accgtactca tcaaaggcga ccagcggtgt gatcagcacg tctaatcggg
    31621 aaagggggag cacgtcacgc acatccagtt ttggctcatg gattttcaac ctgttcatca
    31681 ccagttcgct ttgcggatgg taattcagga acagcaaatt accggcacta aagggatgca
    31741 aaactggaag atatacgcgc ttaccggcgc gccagagttg ttctataagc ggctgggtgt
    31801 cgagttcacc atcaaaagag aggaatacag cgaccgtatg cgccatcacc accgggggat
    31861 aagtcatcat ccgggtagcg gcttgttgac ccatttcctg ctgttgttcc ggcgttaacg
    31921 cacgacgacg ttgccgaatc attttgcgga tttcttgtcg ggataatgtc agtgggagtt
    31981 ctggtagttg cgtcatggct ggtgccagat aagaagggaa tctccgagat gccgccgcag
    32041 gctgtaaccc ttgaaccctt ggttcaaggt gaatgtgtcg tcgcagtttt aaggcttctc
    32101 ggacggaccg agcatgctca ccaaccgcgg agcgccacat tcttgtggta tgaaatatcg
    32161 gctcagggga ctggcccgct tgcgaacatc tcagagaaat tttgtcttca cggttactct
    32221 accacagtaa accgaaaagt gtcattcaaa gttttggtta gttttttcgg tgatgcgacc
    32281 ttgttcaagt aacgcttgtt ctatggtctg ctgcagcatc cgaatacgct gttccatact
    32341 tgccgcgtag tcacgagtct tcgctttttc ttgcgctaac tcatagctga tattcagtgc
    32401 ggcaatgaag accaactgtt cagtatttgt gactctagtg cgttctttca gatcttgcaa
    32461 ccgttggttc agatcgtccg ctgcctgatt caacgcatcc ctttggtcag gcgggcagtt
    32521 cacacgcagt gaacggccaa aaatttggat atcgacgggt tgtgcagaca tgccaccttc
    32581 ctgctgattg actgcgctgc cttcgtcttt agacccatgg tctgcgaagg ggcgacacta
    32641 tagctaccct gatgagaaga gacaagccct tttctggtcc accagggccc aaagtggtag
    32701 catatcatga atattcctcc ctttgacgac gaatgcttat gtctatacag aacgaaatgc
    32761 ctggttacaa cgaaatgaac cagtatctga accaacaagg gacgggtctg acccctgctg
    32821 agatgcatgg tttaatcagt gggatgatat gtggcggtaa cgatgacagc tcatggctac
    32881 cgctacttca cgacctgacg aacgaaggca tggctttcgg tcatgagctg gcacaggcat
    32941 tgcgtaaaat gcactctgcc accagcgatg ccctgcagga tgacggcttc ctttttcagc
    33001 tttatctgcc tgatggcgat gatgtcagcg ttttcgatcg ggctgatgca ttggcaggtt
    33061 gggtcaatca cttcctgctt ggtcttggtg ttacgcaacc gaagctggat aaagtgaccg
    33121 gcgaaaccgg tgaagctatc gacgatctgc gtaacattgc gcaactgggt tacgacgaag
    33181 acgaagatca ggaagagctt gaaatgtcgc ttgaagagat catcgaatac gttcgtgttg
    33241 ccgcgctgtt atgccacgac acctttactc atccgcaacc gaccgcgcca gaagtacaaa
    33301 aaccgactct acactaaaaa caaaaaacgt aaggagagtg ttatgagtga gatatcccgg
    33361 caagagtttc agcgtcgccg tcaggccctg gtggagcaaa tgcaacccgg cagcgccgcg
    33421 ctgatttttg ctgcaccaga agtaacacgt agcgccgaca gcgaataccc ctatcgtcag
    33481 aacagtgact tctggtactt caccggcttt aacgaaccgg aagcggtgtt ggtgctgatt
    33541 aaaagcgatg acactcataa ccacagcgtt ctgtttaacc gcgttcgcga cctgacggcg
    33601 gagatctggt ttggccgtcg cttaggccag gatgccgcgc cagagaaact gggcgttgac
    33661 cgcgcactgg cattcagcga aattaatcag caactttatc aactacttaa tggcctggat
    33721 gtggtttacc atgctcaggg cgaatatgca tatgctgatg aaatcgtgaa cagtgcgctg
    33781 gaaaaactgc gtaaaggttc gcggcaaaat ctcaccgcac cggcaacgat gatcgactgg
    33841 cgtcctgttg ttcatgaaat gcgcctgttc aaatcgccag aagagattgc cgtactccgc
    33901 cgcgcgggag aaatcaccgc catggcacat acacgggcga tggaaaaatg ccgtccggga
    33961 atgttcgagt accatctgga aggcgaaatt caccacgaat ttaaccgcca cggtgcgcgc
    34021 tatccgtcct ataacaccat tgtaggcagc ggtgaaaacg gctgcatttt acactacacc
    34081 gaaaacgagt gtgaaatgcg cgacggcgac ctggtgttga ttgacgcagg ctgcgaatac
    34141 aaaggttacg caggcgatat cacccgtacc ttcccggtca acggcaaatt cacccaggcc
    34201 cagcgcgaaa tctacgacat tgtgctggag tctcttgaaa ccagcctgcg cctgtatcgt
    34261 ccgggaacct ccattctgga agtcaccggt gaagtggtgc gcattatggt tagcggcctg
    34321 gtaaaactcg gcatcctgaa aggtgatatt gatgaactga tcgctcagaa cgcccatcgt
    34381 cctttcttta tgcatggcct tagccactgg ttaggactgg atgtccatga cgttggcgtt
    34441 tatggtcagg atcgctcgcg cattctggaa ccggggatgg tactgaccgt agagcctggt
    34501 ctgtatatcg cgccggatgc ggatgtgcca gaacaatatc gcggtatcgg cattcgtatt
    34561 gaagacgaca ttgtgattac cgaaaccggc aacgaaaacc tcaccgccag cgtggtgaaa
    34621 aagccggaag aaatcgaagc gttgatggct gctgcgagaa agcaatgagc gtaatcatcg
    34681 tcggtggcgg catggcgggc gcgacgctgg cgctggctat ttcccggtta agtcacgggg
    34741 cgctgccggt acatttgatt gaagcgactg cgccagagtc acatgctcat ccgggctttg
    34801 atggacgagc gatagcgctg gcggcgggta cctgtcagca actggcgcgc atcggcgttt
    34861 ggcaatctct ggcggattgc gcaactgcca tcaccaccgt gcatgtcagc gatcgaggtc
    34921 acgctggatt tgtcaccctc gccgcagaag attaccaact ggcggcgctg ggacaggttg
    34981 tcgaattgca caatgtcggg caacggctgt ttgcattgct gcgtaaagca cctggcgtaa
    35041 cgctgcattg ccctgatcgc gtggctaacg ttgcccgtac tcagagtcac gttgaagtga
    35101 cgctggaggg tggcgagacg ctgacgggcc gcgtgctggt agccgctgat ggcacccatt
    35161 cagcgttagc cactgtgtgc ggcgttgact ggcagcagga gccttacgaa caactggctg
    35221 tgattgccaa cgttgcgact tccgttgcgc atgaagggcg cgcttttgaa cgctttacgc
    35281 aacatggccc gctggcgatg ttgccgatgt ctgacggacg ctgttcgctg gtctggtgtc
    35341 atccactgga acggcgcgaa gaggtgctgt cgtggagcga cgagaagttt tgccgtgaac
    35401 tccagtcggc ctttggctgg cgacttggga aaattaccca cgctggtaaa cgcagtgctt
    35461 atccgctggc gttaacccac gccgccagat ctattaccca tcgtaccgtg ctggtgggca
    35521 atgcggcgca aaccctgcac ccgattgccg ggcaagggtt caacctcggt atgcgagatg
    35581 tcatgagtct tgcggaaacc ctgactcagg cgcaggagcg cggagaagac atgggtgatt
    35641 acggcgtatt gtgtcgttat cagcagcgtc gacagagcga tcgcgaagca accattggcg
    35701 tcacggacag ccttgtacat ctttttgcca accgttggac gccactggtt gtcgggcgca
    35761 acatcgggct gatgacgatg gaattattca ccccggcacg cgatgtgctg gcgcagcgca
    35821 ccctcggttg ggtggcgcgt tgaaggcggt tattaaggaa taaacaatgc aaagtgttga
    35881 tgtagccatt gttggcggcg gcatggtggg gctggcggtt gcctgcggct tacagggtag
    35941 cggcttacgc gttgccgtac tggagcagcg cgtaccggaa cctctggcgg cggatgcacc
    36001 accacaactg cgcgtttcgg ctatcaatgc cgccagcgaa aaattactca cccgtcttgg
    36061 cgtctggcag gacattctct ctcgccgagc cagctgttat cacggtatgg aagtgtggga
    36121 caaagacagt tttggtcaca tctcgtttga cgatcaaagc atgggctata gccatcttgg
    36181 gcacatcgtt gaaaactcag tgattcacta cgcgctgtgg aacaaagcgc agcagtcgtc
    36241 agatatcact cttttggccc ccgcagaatt acagcaggtc gcctggggag aaaatgaaac
    36301 cttcctgacg ctgaaagatg gcagtatgtt aacggcgcgt ctggtgattg gcgcggacgg
    36361 cgctaattcc tggttgcgca acaaagccga tattccgctg actttctggg attatcagca
    36421 tcacgcgctg gtagcgacca tacgcacgga agaaccgcat gatgcggtgg cgcggcaggt
    36481 tttccatggc gaaggcattc tggccttttt accgcttagc gatccgcatc tttgctcgat
    36541 tgtctggtca ctgtcgccag aggaagcgca gcggatgcag caggcaagtg aagacgaatt
    36601 taatcgcgcg ttaaatatcg cttttgataa tcgcctgggc ttatgcaagg ttgagagcgc
    36661 gcgtcaggtg ttcccactga cggggcgtta tgcgcgccag tttgccgcgc accgtctggc
    36721 gctggtgggc gacgccgcgc ataccattca cccgctggcg gggcaggggg taaatcttgg
    36781 ctttatggat gctgcagagc tgattgctga actgaaacgg ttgcatcgtc agggtaaaga
    36841 catcgggcag tacatttatc tgcgtcgcta tgagcgtagc cgcaagcaca gtgcggcgct
    36901 gatgctggct ggtatgcagg gattccgcga tctgttttct ggtaccaatc cggtgaaaaa
    36961 actgctgcgt gatattggtc tgaaactggc cgacacgctt cctggcgtta aaccacaact
    37021 tatccgccag gcaatgggat taaacgattt gcctgaatgg ctgcgttaaa aatttctctt
    37081 ctgctgttta tttgataccc atcacacttt catctcccgg ttttttcgcc gggagatttt
    37141 cctcatttga aataaactaa tttcacctcc gttttcgcat tatattttct aatgtcacga
    37201 atttttgatt tagtgttttt tgacattttt tcagctctta atattgcctt attcaaatta
    37261 actttctcat cacatcatct ttatatagaa acttgtgtat tttttggttt tttattctgt
    37321 tgcgattttg gcgtttttta accataagct aatgtgatga tcaattttac cttatggtta
    37381 acagtctgtt tcggtggtaa gttcaggcaa aagagaacga ttgcgttggg gaccgggagt
    37441 ggctccgatg ctgggtttcg tggtgataat ttcaccatga aaaagttgtc agccccgctt
    37501 attcaatgag gacaagatgg cacaacagac tcctttgtac gaacaacaca cgctttgcgg
    37561 cgctcgcatg gtggatttcc acggctggat gatgccgctg cattacggtt cgcaaatcga
    37621 cgaacatcat gaggtacgta ccgatgccgg aatgtttgat gtgtcacata tgaccatcgt
    37681 cgatcttcgc ggcagccgca cccgggagtt tctgcgttat ctgctggcga acgatgtggc
    37741 gaagctcacc aaaagcggca aagcccttta ctcggggatg ttgaatgcct ctggcggtgt
    37801 gatagatgac ctcatcgtct actactttac tgaagatttc ttccgcctcg ttgttaactc
    37861 cgccacccgc gaaaaagacc tctcctggat tacccaacac gctgaacctt tcggcatcga
    37921 aattaccgtt cgtgatgacc tttccatgat cgccgtacaa gggccgaatg cgcaggcaaa
    37981 agctgccaca ctgtttaatg atgctcagcg tcaggcggtg gaagggatga aaccgttctt
    38041 tggcgtgcag gcgggcgatc tgtttattgc caccactggt tataccggtg aagcgggcta
    38101 tgaaattgcg ctgcccaatg aaaaagcggc cgatttctgg cgtgcgctgg tggaaggggg
    38161 cgttaagcca tgcggtctgg gcgcgcgtga cacgctgcgt ctggaagcgg gcatgaatct
    38221 ttatggtcag gagatggacg aaaccatctc tccgttagcc gccaacatgg gctggactat
    38281 cgcctgggaa ccggcagatc gtgacttcat cggtcgtgaa gccctggaag tgcagcgtga
    38341 gcatggcaca gaaaaactgg ttggtctggt gatgaccgaa aaaggcgtgc tgcgtaatga
    38401 actgccggta cgttttaccg atgcgcaggg caaccagcat gaaggcatta tcaccagcgg
    38461 gactttctcc ccgacgctgg gttacagcat tgcgctggca cgcgtgccga aaggtattgg
    38521 cgaaacggcg attgtgcaaa ttcgcaaccg tgaaatgccg gttaaagtga caaaacctgt
    38581 ttttgtgcgt aacggcaaag ccgtcgcgtg atttactttt ttggagattg attgatgagc
    38641 aacgtaccag cagaactgaa atacagcaaa gaacacgaat ggctgcgtaa agaagccgac
    38701 ggcacttaca ccgttggtat taccgaacat gctcaggagc tgttaggcga tatggtgttt
    38761 gttgacctgc cggaagtggg cgcaacggtt agcgcgggcg atgactgcgc ggttgccgaa
    38821 tcagtaaaag cggcgtcaga catttatgcg ccagtaagcg gtgaaatcgt ggcggtaaac
    38881 gacgcactga gcgactcccc ggaactggtg aacagcgaac cgtatgcagg tggctggatc
    38941 tttaaaatca aagccagcga tgaaagcgaa ctggaatcac tgctggatgc gaccgcatac
    39001 gaagcattgt tagaagacga gtaacggctt tattcctctt ctgcgggaga ggatcagggt
    39061 gaggaaattt acacctcacc ctcactctct tcgcaaggag agaggttcac aattcactgc
    39121 acgtttcagg aaccatcgct catgacacag acgttaagcc agcttgaaaa tagcggcgct
    39181 tttattgaac gccatatcgg accggacgcc gcgcaacagc aagaaatgct gaatgccgtt
    39241 ggcgcacaat cgttaaacgc gctgaccggc cagattgtac cgaaagatat tcagcttgcg
    39301 accccgccgc aggttggcgc accggcgacc gaatacgccg cgctggcgga actcaaggct
    39361 attgccagtc gcaataaacg cttcacgtct tacatcggca tgggttacac cgccgtgcag
    39421 ttaccgccgg ttatcctgcg taacatgctg gaaaatccgg gctggtatac cgcgtatact
    39481 ccgtatcaac ctgaagtctc gcagggccgc cttgaggcac tgctcaattt ccagcaggta
    39541 acgctggatt taaccgggct ggatatggcc tctgcttctc ttcttgacga agccaccgct
    39601 gccgccgaag caatggcgat ggcgaaacgc gtcagcaaac tgaaaaatgc caaccgcttc
    39661 ttcgtggctt ccgatgtgca tccgcaaacg ctggatgtgg tccgtactcg tgccgaaacc
    39721 tttggttttg aagtgattgt cgatgatgcg caaaaagtgc tcgaccatca ggacatcttc
    39781 ggcgtgctgt tacagcaggt tggcactacc ggtgaaattc acgactacac tgcgcttatc
    39841 agcgaactga aatcacgcaa aattgtggtc agcgttgccg ctgatattat ggcgctggtg
    39901 ctgttaactg cgccgggtaa acagggcgcg gatattgttt ttggttcggc gcaacgcttt
    39961 ggcgtaccga tgggttacgg tggcccacac gcggcattct ttgcggcgaa agacgaatac
    40021 aaacgctcaa tgccaggccg tattatcggt gtatcgaaag atgcagctgg caataccgcg
    40081 ctgcgcatgg cgatgcagac tcgcgagcaa catattcgtc gtgagaaagc gaactccaac
    40141 atttgtactt ctcaggtgct gctggcaaac atcgccagcc tgtatgccgt ttatcacggc
    40201 ccgattggcc tgaaacgtat cgctaaccgc attcaccgtc tgaccgatat cctggcggca
    40261 ggcctgcaac aaaaaggtct gaagctgcgc catgcgcact atttcgacac tctgtgtgtg
    40321 gaagtggccg acaaggcggg cgtactggcg cgtgccgaag cggctgaaat caacctgcgt
    40381 agcgatatcc tgaacgcggt tgggatcacc cttgatgaaa ccaccacgcg cgaaaacgtg
    40441 atgcagcttt tcagcgtact actgggggat aaccacgggc tggacatcga cacgctggac
    40501 aaagacgtgg ctcacgacag ccgctctatc cagcctgcga tgctgcgcga cgacgaaatc
    40561 ctcacccatc cggtgtttaa tcgctaccac agcgaaaccg aaatgatgcg ctatatgcac
    40621 tcgctggagc gtaaagatct ggcgctgaat caggcgatga tcccgctggg ttcctgcacc
    40681 atgaaactga acgccgccgc cgagatgatc ccaatcacct ggccggaatt tgccgaactg
    40741 cacccgttct gcccgccgga gcaggccgaa ggttatcagc agatgatcgc gcagctggct
    40801 gactggctgg tgaaactgac cggttacgac gccgtttgta tgcagccgaa ctctggcgca
    40861 cagggcgaat acgcgggcct gctggcgatt cgtcattatc atgaaagccg caacgaaggg
    40921 catcgcgata tctgcctgat cccggcttct gcgcacggca ctaaccccgc ttctgcacat
    40981 atggcaggaa tgcaggtggt ggttgtggcg tgtgataaaa acggaaatat cgatctgact
    41041 gatctgcgcg cgaaagccga acaggcaggt gataatctct cctgcatcat ggtgacctat
    41101 ccttccaccc acggtgtgta tgaagaaacg atccgtgaag tgtgtgaagt cgtgcatcag
    41161 ttcggcggtc aggtttacct tgatggcgcg aacatgaacg cccaggttgg catcacctcg
    41221 ccgggcttta ttggcgcaga cgtttcacac cttaacctgc ataaaacttt ctgcattccg
    41281 cacggcggtg gtggtccggg tatgggaccg atcggcgtga aagcgcatct ggctccgttt
    41341 gtaccgggtc atagcgtggt gcaaatcgaa ggcatgttaa cccgtcaggg cgcggtttct
    41401 gcggcaccgt tcggtagcgc ctctatcctg ccaatcagct ggatgtacat ccgcatgatg
    41461 ggcgcagaag ggctgaaaaa agcaagccag gtggcaatcc tcaacgccaa ctatattgcc
    41521 agccgcctgc aggatgcctt cccggtgctg tataccggtc gtgacggtcg cgtggcgcac
    41581 gaatgtattc tcgatattcg tccgttgaaa gaagaaaccg gcatcagcga gctggatatt
    41641 gccaagcgcc tgatcgacta cggtttccac gcgccgacga tgtcgttccc ggtggcaggt
    41701 acgctgatgg ttgaaccgac tgaatctgaa agcaaagtgg aactggatcg ctttatcgac
    41761 gcgatgctgg ctatccgcgc agaaattgac caggtgaaag ccggtgtctg gccgctggaa
    41821 gataacccgc tggtgaacgc gccgcacatt cagagtgaac tggtcgccga gtgggcgcat
    41881 ccgtacagcc gtgaagttgc ggtattcccg gcaggcgtgg cagacaaata ctggccgaca
    41941 gtgaaacgtt tggatgatgt ttacggcgac cgtaacctgt tctgctcctg cgtaccgatt
    42001 agcgaatacc agtaattcac tgattcgact atcttctaaa ggcgcttcgg cgccttttta
    42061 gattgttaat aaagtgcgct ttgtgtgttt cggctgaggc gtaatataaa atattgcgag
    42121 ctcaatatat taaaagagtg ccaggcctga tgagcgtagc gcatcaggca catcggctaa
    42181 ttacagattc tcgccgttgc tggcaatcac ttctttgtac cagttaaagc tcttcttacg
    42241 tgaacgcgac atatcgccag taccgtcgtc atgtttattc acatagataa agccgtagcg
    42301 tttgctgtac tgcccggtag tgaacgacac gcagtcgatg cagccccacg gtgtgtagcc
    42361 catcagatcc acgccatcgt aagttactgc ttttttcatc tcttcaatat gggcgcgcag
    42421 gtagtcaatg cggtagtcgt cgttgatgct gccatcttct tccactttgt cgtaagcgcc
    42481 aaaaccgttt tcgacaataa acagcggctt ctggtaacgc tcatacagtt cgcaaagtgc
    42541 gtaacgcaga cccaccggat caatctgcca gccccagtca gatgctttaa catacgggtt
    42601 tggtacgctg ccttcaaaac cagagatcgc atcgccggtg ccgccttcgg ccttcactgc
    42661 gttggtcatg taatagctga aaccaagata atcgcaggtg ccttcgcgca gtacatcgag
    42721 atcgctatct tccattttga tgttaaatcc gcgacgctcc cactcgttca acacatacga
    42781 cgggtaatag ccgcgtagct gcacatcggt aaagacgtag cgttcgcgca tcgactcctg
    42841 agcgaacatc acatcgtccg ggttacatga gtagggatag agcggcacca tcgccagcat
    42901 acagccgact ttcatctcca ggttaatgcg acgcgcggct ttcactgcca gggcgctggc
    42961 gacaaactgg tgatgcagca cctgatacat cgtctcttcc gggttttcat gctcggtata
    43021 caccacgccg gagcagcagt aaccgaacag cggtgcacgc cagttacgct ggttgttaat
    43081 ttcgttgaag gtcatccagt atttgacttt gtgcttatag cgttcaaata ccacttccgc
    43141 gaaacgtaca aagaaatcaa ccactttacg gttggtccag ctaccgtatt gctgcaccag
    43201 atgcagcggc atttcaaagt gggagagggt gatcaccggt tcgatgttgt atttcagcag
    43261 ttcatcgaac agagagtcgt agaacttcag cccttcttcg tttggctgag cttcatcgcc
    43321 ttttggaaaa atgcgcgtcc aggcaatgga tgtacgaaaa catttgaagc ccatttcggc
    43381 aaatagcttg atgtcttcct tatagtgacc ataaaaatca acggcttcat ggtttggata
    43441 gtatttgcct ggcaagactt ctttggttat ttcgcgcggc acgccgtgtg cgccaccggt
    43501 cagaacgtca caaatgctcg gcccttttcc gcctttgttc cagccgcctt cgacctgatg
    43561 agcggcaact gcgccgcccc ataagaaatc tttcggtaag gtgagttttt tcactatcat
    43621 aggctccaga tgagtcaatt cttacaggaa atagtaccat gcagccaaac gacatcactt
    43681 tttttcaacg tttccaggat gacattctgg ctgggcgtaa aaccatcacc atccgcgacg
    43741 agtctgaatc gcacttcaaa acgggtgatg tgcttcgtgt cggacgtttt gaagatgacg
    43801 gttatttttg cacgattgaa gtcaccgcaa cctcaaccgt aacgctggat acgctgacgg
    43861 aaaaacatgc agagcaggaa aatatgaccc tgactgaact gaaaaaggtc attgccgaca
    43921 tctatcccgg ccagacacag ttttatgtga ttgaatttaa atgcctttaa attgaactca
    43981 cgcctgttag ttgaaatttc gcatttaatt tcatgatttt taggagatta gtctcaaatc
    44041 agaatttatt ttagctaaca ggtgttcact ggaactattc tcagttacgc tggagaggta
    44101 tacatgtgcg tgtacgtttc tccggagtaa gttatggttc agaagcccct cattaagcag
    44161 ggatattcgc tggcagagga aattgccaac agcgtcagtc acggcattgg gttggtgttt
    44221 ggtatcgttg ggctggtgtt gctactggtt caggcggtgg atctcaatgc cagcgccaca
    44281 gcgataacca gctacagcct ctatggcggc agtatgatcc tgctgttcct cgcttcgacg
    44341 ctctatcacg ccattcctca tcaacgggca aaaatgtggc tgaagaaatt tgaccattgc
    44401 gctatttacc tgttgattgc cggaacctac acgccgtttt tgctggtggg gctggattct
    44461 ccgttagcgc gcgggttgat gattgttatc tggagcctgg cattgctggg gattctgttc
    44521 aaactgacca tcgcgcaccg attcaaaatt ttatctctgg tgacttatct ggcgatgggc
    44581 tggctgtcgc tggtggtaat ttatgaaatg gcagttaagc tcgcggcggg cagcgttacc
    44641 ttactggcgg taggcggcgt ggtttattcg ctcggggtga ttttctacgt ctgcaaacgc
    44701 attccataca accatgccat ctggcacggc ttcgtgctcg gcggtagtgt gtgccacttt
    44761 ctggcgatct atttgtatat tgggcaggcg taatgggtat gcctttgtag gcctgataag
    44821 acgcgccagc gtcgcatcag gcgataaatg cctgatgcga cacagagatt tactcttcga
    44881 gcgaatacgg cagtggctcg atacacaatg tattcgcatc gtcgcgcacg cggaagatgc
    44941 tgtccggttc catatcgtta ttcatcacta cctgcaccac gacctgacca tcttccagtt
    45001 ttaccgcagc cagcaccgta ccagtacgac gccagttctc gcccattttc agctctaagt
    45061 cttcaccagc ttccggcagt cggctggcac tacctgtcag caaccagagc gcgcgtttgt
    45121 tggcaccacg gaattttgct cgcgccacca tctcttgtcc ggtatagcag cctttcttaa
    45181 agctgatacc gcccagcgcc tggaggttgg tcgcctgtgg gataaactgc ccgctgttcg
    45241 cggcatcaat caccgggaaa cccgcttcaa tgtttaatgc cagccactgt tggctattgt
    45301 tcagttccgc ttcaccgcgc agtttatcgg tcagcatatt ggcagtagct tcatcggtta
    45361 cgatcaggaa acgttctgcc gggtgttcaa accatagcaa agtggtcgcg ccttctttaa
    45421 ctacctgttt ttctttcgaa ggcagttcac taaagatatt tgccagcgcg gcgcgcgcct
    45481 gaaaaccggc aacaccaagc agcacacgct cgtcgtctgg cgcgatggtc actttagaga
    45541 acaccgcata ttttttcagt tcagccagct gcggttcacg cacgctgcgc cgttcaatcc
    45601 atgcaaagcc atcgccgtcg cggaacagac gtaaattgct ccacatttta cctttggcgt
    45661 cgcaatgggc ggcgagcagg tgctgatcat ctgtcatctg gctgacatct gctgtcacct
    45721 ggccctgcaa atatttttcg ctgtccgcgc cagtaatggt ggcaagcgcc cagtcatcaa
    45781 gcgtcatcag cgtcagtggc aaacgggcag aagccgtagg ctgacgggga ggaaaaggtg
    45841 taaaagccat aatagagtcc tgagttgctt aacggcgtat taataactaa tggtaaaaga
    45901 gctatgaggc aatgcaagtg ctttcactac gcgatctgtg ccttctgcga ggcgacttcc
    45961 caaaaaatga agcccttaaa ttgttgtgaa ttaatttcgc gatcgcgatt gtgtttactg
    46021 atattcccgt aaaagaagtc gaaaaacacg ttacaatagc cgggtactgg attttcgtga
    46081 gacaggaaga agatggacat taacaacaaa gcccgcattc attgggcatg ccgccgtggt
    46141 atgcgcgaac tcgatatttc aatcatgccg tttttcgaac atgagtatga cagcttaagc
    46201 gatgacgaaa aacgcatctt tattcgtctg ctggaatgtg acgatcccga tctgtttaac
    46261 tggctgatga atcacggtaa accggccgat gcagaactgg aaatgatggt ccgactcatc
    46321 cagacacgga accgggaacg tggtcctgtg gcaatctgat ttgcgcgtct cctggcgcgc
    46381 acagtggctt tccttgctga ttcatgggct ggttgccgct gttattttac tcatgccctg
    46441 gccgctcagt tacaccccgt tatggatggt gttactttcg ctggtggtgt ttgattgcgt
    46501 tcgcagccag cggcgtatta atgctcgcca gggggaaatt cgcttgttga tggacgggcg
    46561 tttgcgttgg caagggcagg agtggagcat cgtcaaagcg ccgtggatga ttaagagcgg
    46621 catgatgctg cgtttacgtt ctgatagcgg taaacggcaa catttatggc tggcagccga
    46681 cagcatggac gaagccgaat ggcgggattt acggcggatt ttgttgcaac aagagacgca
    46741 aagataaaga tgacggcaaa gcatatgcgc taagccgtca gtgatatcag gcgtaatgct
    46801 ctgccatttc gttgaggatt tgctcgcacc agctctgaat acgctcgtcg ctaaggtcat
    46861 actggttagt ttcgtccagc gccagcccca cgaacagttg cccgtcagca atcaccggtt
    46921 tcgggctggt aaattcatac ccttccgttg gccagtagcc gacgaacttc acgcctttgg
    46981 tcgagagttt gtcatgcagc ataccgagcg catcgaggaa ccactcgccg tatcccagtt
    47041 gatcgccaag cccatacagc gcaacaattt taccttcgag gttcaggtcg tcgagctgat
    47101 cccagacggc ttcccagtct tcctggattt cgccaaaatc ccaggtcggg atacccagaa
    47161 tgagcacatc gtactgctcc attaatttcg gggagtcgtc cttgaggtta tgtaaggtca
    47221 ccagttctgg gccgataata tcgcggattt tttctgccgc catttcggtg taacaggtgc
    47281 tggaaccgta aaaaagaccc atattcataa cgtaagcttc tcgattctgc tgtggctttt
    47341 gcggctagtg tatcagaatc gctttagtca cggcacaata aggcataatg gatcaaatga
    47401 gtgaccataa ggggcgcaag tgaaacagga tctggcacgc atcgagcagt ttcttgatgc
    47461 tctgtggctg gaaaaaaatc tggctgaaaa tacgttgaac gcttaccgtc gcgatctgtc
    47521 aatgatggtg gagtggttgc atcaccgcgg gttgacgctg gcgacggcgc aaagtgacga
    47581 tttacaggca ttactggcag aacggctgga gggcgggtat aaagccacca gctcagcgcg
    47641 tttgctgagt gcagtgcgcc gattgttcca gtatctttat cgcgaaaagt ttcgtgaaga
    47701 tgatcccagt gcgcatctcg cttcaccgaa attgccccag cgtttgccaa aagatttaag
    47761 cgaagcgcag gtcgaacgtt tattacaggc accattaatt gatcagccac tggagctacg
    47821 cgataaagct atgcttgaag tgttgtatgc taccgggctg cgtgtctctg aactggtcgg
    47881 gctgacaatg agtgatatca gcctgcgtca gggcgtagtg cgggtcattg gtaaaggcaa
    47941 caaagagcgc ctggtgccgt taggtgaaga ggcggtttac tggctggaaa cctatctgga
    48001 acatgggcgt ccgtggctac tgaatggtgt gtcaattgac gtgttgtttc ccagccagcg
    48061 tgcgcagcag atgacgcgac agaccttctg gcaccgcatt aaacattatg ccgtgctggc
    48121 gggtatcgac agcgaaaagc tgtcaccgca tgtgttgcgt cacgcttttg ccactcactt
    48181 attaaatcat ggtgcggatt tacgcgtggt gcagatgcta ctgggccaca gcgatctctc
    48241 caccacgcaa atttatacgc atgtcgctac cgagcgtctg cggcaacttc atcaacagca
    48301 tcacccgcgg gcgtgatgtc tgaaaagaac gggaagattt atgaagaaag gttttatgtt
    48361 gtttactttg ttagcggcgt tttcaggctt cgctcaggct gatgacgcgg caattcaaca
    48421 aacattagcc aaaatgggca tcaaaagcag cgatattcag cccgcgcccg tagctggcat
    48481 gaagactgtg ctgactaaca gcggtgtgtt gtacatcacc gatgatggta aacatatcat
    48541 tcaggggcca atgtatgacg ttagcggcac ggctccggtc aatgtcacca ataagatgct
    48601 gttaaagcag ttgaatgcgc ttgaaaaaga gatgatcgtt tataaagcac cgcaggaaaa
    48661 acacgtcatc accgtgttta ctgatattac ctgtggttac tgccacaaac tgcatgaaca
    48721 aatggcagac tataacgcgc tggggatcac cgtgcgttat cttgctttcc cacgccaggg
    48781 gctggacagc gatgctgaga aagaaatgaa agctatctgg tgtgcgaaag ataaaaacaa
    48841 agcgtttgat gatgtgatgg caggtaaaag cgtcgcacca gccagctgcg acgtggatat
    48901 tgccgaccat tacgcacttg gcgtccagct tggcgttagc ggtactccgg cagttgtgct
    48961 gagcaatggc acacttgttc cgggttacca gccgccgaaa gagatgaaag aattcctcga
    49021 cgaacaccaa aaaatgacca gcggtaaata attcgcgtga aacaacagat acaacttcgt
    49081 cgccgtgaag tcgatgaaac ggcagacttg cccgcggaat tgcctccctt gctgcgccgt
    49141 ttatacgcca gccggggagt acgcagtgcg caagaactgg aacgcagtgt taaaggtatg
    49201 ctgccctggc agcaactgag cggcgtcgaa aaggccgttg agatccttta caacgctttt
    49261 cgcgaaggaa cgcggattat tgtggtcggt gatttcgacg ccgacggtgc caccagcacg
    49321 gctctaagcg tgctggcgat gcgctcgctt ggttgcagca atatcgacta cctggtacca
    49381 aaccgtttcg aagacggtta cggcttaagc ccggaagtgg tcgatcaggc ccatgcccgt
    49441 ggcgcgcagt taattgtcac ggtggataac ggtatttcct cccatgcggg agttgagcac
    49501 gctcgctcgt tgggcatccc ggttattgtt accgatcacc atttgccggg cgacacatta
    49561 cccgcagcgg aagcgatcat taaccctaac ttgcgcgact gtaatttccc gtcgaaatca
    49621 ctggcaggcg tgggtgtggc gttttatctg atgctggcgc tgcgcacctt tttgcgcgat
    49681 cagggctggt ttgatgagcg cggcatcgca attcctaacc tggcagaact gctggatctg
    49741 gtcgcgctgg ggacagtggc ggacgtcgtg ccgctggacg ctaataatcg cattctgacc
    49801 tggcagggga tgagtcgcat ccgtgccgga aagtgccgtc cagggattaa agcgctgctg
    49861 gaagtggcaa accgtgatgc acaaaaactc gccgccagcg atttaggttt tgcgctgggg
    49921 ccacgtctca atgctgccgg gcgactggac gatatgtccg tcggtgtggc gctgttgctg
    49981 tgcgacaaca tcggcgaagc gcgcgtgctg gcaaatgaac tcgatgcgct aaatcagacg
    50041 cgaaaagaga tcgaacaagg aatgcagatt gaagccttga ccctgtgcga gaaactggag
    50101 cgaagtcgcg acacgctacc cggcgggctg gcaatgtatc accccgaatg gcatcagggc
    50161 gttgtcggta ttctggcttc gcgcatcaaa gagcgttttc accgtccggt tatcgccttt
    50221 gcgccagcag gtgatggtac gctgaaaggt tccggtcgct ccattcaggg gctgcatatg
    50281 cgtgatgcgc tggagcgatt agacacactc taccccggta tgatgctgaa gtttggcggt
    50341 catgcgatgg cggcgggttt gtcgctggaa gaggataaat tcgaactctt tcaacaacgg
    50401 tttggcgagt tggttaccga gtggctggac ccttcgctat tgcaaggcga agtggtgtca
    50461 gacggcccgt taagcccggc cgaaatgacc atggaagtgg cgcagctgct gcgcgatgct
    50521 ggcccgtggg ggcagatgtt cccggagccg ctgtttgatg gtcatttccg tctgctgcaa
    50581 cagcggctgg tgggcgaacg tcatttgaaa gtcatggtcg aaccggtcgg cggcggtccg
    50641 ctgctggatg gtattgcttt taatgtcgat accgccctct ggccggataa cggcgtgcgc
    50701 gaagtgcaac tggcttacaa gctcgatatc aacgagtttc gcggcaaccg cagcctgcaa
    50761 attatcatcg acaatatctg gccaatttag cgtcatcttc tctataaaaa agagcgtgga
    50821 ttgggtacaa tcccgctctt atcaccgcat tttgactagc tcaataaaag aaatcagacc
    50881 atgtttgaaa ttaatccggt aaataatcgc attcaggacc tcacggaacg ctccgacgtt
    50941 cttagggggt atctttgact acgacgccaa gaaagagcgt ctggaagaag taaacgccga
    51001 gctggaacag ccggatgtct ggaacgaacc cgaacgcgca caggcgctgg gtaaagagcg
    51061 ttcctccctc gaagccgttg tcgacaccct cgaccaaatg aaacaggggc tggaagatgt
    51121 ttctggtctg ctggaactgg ctgtagaagc tgacgacgaa gaaaccttta acgaagccgt
    51181 tgctgaactc gacgccctgg aagaaaaact ggcgcagctt gagttccgcc gtatgttctc
    51241 tggcgaatat gacagcgccg actgctacct cgatattcag gcggggtctg gcggtacgga
    51301 agcacaggac tgggcgagca tgcttgagcg tatgtatttg cgctgggcag aatcgcgtgg
    51361 tttcaaaact gaaatcatcg aagagtcgga aggtgaagtg gcgggtatta aatccgtgac
    51421 gatcaaaatc tccggcgatt acgcttacgg ctggctgcgt acagaaactg gcgttcaccg
    51481 cctggtgcgt aagagcccgt ttgactccgg cggtcgtcgc cacacgtcgt tcagctccgc
    51541 gtttgtttat ccggaagttg atgatgatat tgatatcgaa atcaacccgg cggatctgcg
    51601 cattgacgtt tatcgcgcgt ccggcgcggg cggtcagcac gttaaccgta ccgaatctgc
    51661 ggtgcgtatt acccacatcc cgaccgggat cgtgacccag tgccagaacg accgttccca
    51721 gcacaagaac aaagaccagg ccatgaagca gatgaaagcg aagctttatg aactggagat
    51781 gcagaagaaa aatgccgaga aacaggcgat ggaagataac aaatccgaca tcggctgggg
    51841 cagccagatt cgttcttatg tccttgatga ctcccgcatt aaagatctgc gcaccggggt
    51901 tgaaacccgc aatacgcagg ccgtgctgga cggcagcctg gatcaattta tcgaagcaag
    51961 tttgaaagca gggttatgag gaaccaacat gtctgaacaa cacgcacagg gcgctgacgc
    52021 ggtagtcgat cttaacaatg aactgaaaac gcgtcgtgag aagctggcga acctgcgtga
    52081 gcagggaatt gccttcccga acgatttccg tcgcgatcat acctctgacc aattgcacgc
    52141 agaattcgac ggtaaagaga acgaagaact tgaagcgctg aacatcgaag tcgccgttgc
    52201 tggccgcatg atgacccgcc gtattatggg taaagcctct tttgttaccc tgcaggacgt
    52261 tggcggtcgt attcagctgt acgttgcccg tgacgatctt ccggaaggcg tttacaacga
    52321 gcagttcaaa aaatgggacc tcggcgacat cctcggcgcg aaaggtaaac tgttcaaaac
    52381 caaaaccggc gaactgtcta tccactgtac cgagttgcgt ctgctgacca aagcactgcg
    52441 tccgctgccg gataaattcc acggcttgca ggatcaggaa gcgcgctatc gtcagcgtta
    52501 tctcgatctc atctccaacg atgaatcccg caacaccttt aaagtgcgct cgcagatcct
    52561 ctctggtatt cgccagttca tggtgaaccg cggctttatg gaagttgaaa cgccgatgat
    52621 gcaggtgatc cctggcggtg ccgctgcgcg tccgtttatc acccaccata acgcgctgga
    52681 tctcgacatg tacctgcgta tcgcgccgga actgtacctc aagcgtctgg tggttggtgg
    52741 cttcgagcgt gtattcgaaa tcaaccgtaa cttccgtaac gaaggtattt ccgtacgtca
    52801 taacccagag ttcaccatga tggaactcta tatggcttac gcagattaca aagatctgat
    52861 cgagctgacc gaatcgctgt tccgtactct ggcacaggat attctcggta agacggaagt
    52921 gacctacggc gacgtgacgc tggatttcgg taaaccgttc gaaaaactga ccatgcgtga
    52981 agcgattaag aaatatcgcc cggaaaccga catggcggat ctggacaact tcgactctgc
    53041 aaaagcgatt gttgaatcta tcggtatcca cgttgagaag agctggggtc tgggccgtat
    53101 cgttaccgag atcttcgaag aagtggcaga agcacatctg attcagccga ccttcattac
    53161 tgaatatccg gcagaagttt ctccgctggc gcgtcgtaac gacgttaacc cggaaatcac
    53221 agaccgcttt gagttcttca ttggtggtcg tgaaatcggt aacggcttta gcgagctgaa
    53281 tgatgcggaa gatcaggcgc aacgcttcct ggatcaggtt gccgcgaaag atgcaggtga
    53341 cgacgaagcg atgttctacg acgaagatta cgtcaccgca ctggaacatg gcttaccgcc
    53401 gacagcaggt ctggggattg gtatcgaccg tatggtaatg ctgttcacca acagccatac
    53461 catccgcgac gttattctgt tcccggcgat gcgtccggtg aaataagcat tacgttatgc
    53521 gcataacccc ggcagatgtc ggggtttttt aattgtgctg cgcgaaagca gacaacaatt
    53581 ttcttgcttc actattggct gcctgcatca ccatccacgg actgaacgcc cacggcgtgg
    53641 catcaatacc gtgtaataca tctgctaaat cacaccattg ataatccatc acttcatcat
    53701 cgttgatctg taacgcactg gtggtgcgtg cggcaaatac cggacacact tcattttcca
    53761 caatgccatt cggatcggtg gcgcgatagc gaaagtcagg atagatagat tcaggaggcg
    53821 taatttccac gccaagctca taacggcaac ggcggatcac cgcgtcttcg ttgctttctc
    53881 ccagttgtgg gtgcccacaa accgagttag tccacacgcc aggccatgct tttttgctca
    53941 gtgcgcggcg ggtaactaat aattgtcctt tggcattaaa cagccaactg gagaacgcga
    54001 gatgtaagcg ggtgtctgcc gtgtgtgcgg catacttttc cagcgtaccc gtgggaactc
    54061 cctgtgcatt caataaaatg acgtgttccg tttgcataat ttctcacatg taattctgat
    54121 cactcatcat tatagataat tgaagagtga aatgattaac caagtgagcg tttatcgaca
    54181 accgcccgtt ttgagcggat gccgacaggt aaaaaccatt taaaaaagta gcatcatata
    54241 tttgaagcga ctttcaatgg ccattgcaac acagtcagaa atgacaggaa tagagagagt
    54301 aaaaagcaac agcagactga atatagcgcg cattaaaaaa ttcatattat tctccatgct
    54361 catttttctt cagcttgcgc atcagaagcc acatacgtac tgtcctgact tttaaatctt
    54421 tatcagagac ggattccttc accaggtcag catgatgctg atagccatta aagaagacgt
    54481 tcagcacaac ggcgctaagg gttgcgagca taatgccgct atgtagcagc ggttgcagta
    54541 cggccggtaa cttagaaaag aaatcgtgag agagcgttgg agtcatgcca acgccgagac
    54601 tgatcgccac aatatagagg ttgtaacggt tggtggtgta gttacagcgc gacagaattc
    54661 gaatccctgt cgccagtacc atgccgaaca tcaccagacc agcgccgccc agcacaaatt
    54721 gcggaatgga ggctaccagc accgccattt ttggcaccat gccaaacagg attaatataa
    54781 ttcccgaaga aatacacacc cagcggctat gaacgcgcgt cacgctaacc aggccaacgt
    54841 tttgagaaaa tgacgtgtgg gggaagctgt taaacgtgcc accaatcatt gtccctacgc
    54901 catcgacacg cagcccgcga ataatatcgt gcgaagagag tttacgaccg actatttcac
    54961 ccagtgccag gaacatcccc atcgactcga taaacacgat gattaacacg gcagtcatgg
    55021 tcagaatgga aacgggatcg aaaatcggca taccaaacga catcggggtg acaatcgcaa
    55081 accacgaggc atcatgtagc ccggataaat tgacttcatt catcatccac gaaagtaaaa
    55141 agccaaatac aatccccagt aatacggcga cgttggacat aaatcctttc gcatagcgag
    55201 taatgagcaa gataaaaatt aagacggcaa aggagatacc taaataaacg ggattaccat
    55261 attgcggatt ccctttacct ccggcggccc agtcaatacc cacctgaatg atgctaagcc
    55321 cgatagaagt aataaccaca ccggtaacca gtggcgggaa taaaggcatc aagcgaccga
    55381 taagttgcgc taataatgtg gtgataaaac ccgcggcgat agtggcacca aatataccca
    55441 gcaggccgat atccgggttc atcccaatgg ctatcattgg tgttacagca gcaaaggtca
    55501 ccgacataat caccggcagg cggatcccca taaagcggcc gataccgata cattgcaata
    55561 atgtgacgat cccgcagcaa aagagatccg agctaatgag catcgcaata gcttcttttg
    55621 agaggcccag tcggtcacca atcataagag gaacagcgac tgcacctgcg tacatgacaa
    55681 ggacgtgttg cagaccgagt atgatcagct ttcctggtga taatatgcgg tcaacctcat
    55741 cagttgggcg gtcttgccca gaagatgagg gaagttggga atctatggcg ctcatcagtg
    55801 tttctccttt cgcccgccag agtaaaaagc aaccgtttac gttaatgaaa ctgtctaaac
    55861 acatgctcgt ggagtgccag tttttgcgag gaaggggagt tagagacagg aattgcggag
    55921 gcaatcacat aatcgtgctt aaactacgaa aatgacaaga gaacctgaca atggaattat
    55981 ctgtaatttt ctcatattga tatttcatta atcattctgg catgtcgcac tctcgcattt
    56041 aatcgttttt atctggatag cgctatttgg ggcggcaatg ttgaaataat gaaagcggca
    56101 tagattgagg ggcaataatt tgaactcagt cgcaaaatta taattaaaac ggtcatgcta
    56161 cctaatatat gcccgtttat tgcatcgcga aatttgagaa gacataactc attaaatgaa
    56221 agggatgcaa atgaataagt ttatcgctgc tgaagccgcg gaatgtatag gctgtcatgc
    56281 ttgtgaaatt gcctgtgcgg tggcacacaa tcaagaaaac tggccgctga gtcacagtga
    56341 ctttcgacca cgtatccacg ttgtagggaa aggcctggct gcgaatccgg tggcctgcca
    56401 tcactgcaac aatgcccctt gcgttacggc ttgtccggtt aatgctctga ctttccagtc
    56461 cgatagcgta caactggacg agcaaaaatg tattggttgt aaaagatgcg caatcgcttg
    56521 cccctttggc gtcgttgaga tggtcgatac gattgcgcag aaatgcgacc tttgtaacca
    56581 gcgcagttcc ggcacgcaag cctgtattga agtttgccca acacaggcgt tacgactgat
    56641 ggacgataaa gggttacagc agataaaggt ggcccgccag cgcaaaacgg cagcaggaaa
    56701 agcgtcatca gacgctcagc catctcgcag tgcagcgttg ctccccgtta actcgcgtaa
    56761 aggcgcagat aaaatttcag caagtgaacg gaaaaaccac tttggcgaaa tctattgcgg
    56821 gctggatcca caacaagcga cttatgagag tgaccgctgt gtttattgtg ccgaaaaagc
    56881 taactgcaac tggcattgcc cgctgcataa cgctattccg gattacatcc gtctggtaca
    56941 ggagggaaag attattgaag cggcagaact ttgccaccag accagttcct taccagaaat
    57001 ctgcggcagg gtatgtccac aggaccgtct ttgtgaaggc gcatgtactt tgaaagatca
    57061 ctctggcgca gtctctatcg gtaatctgga acgctacatc accgataccg cgctggcgat
    57121 gggctggcgt cccgatgtca gcaaagttgt tccccgtatc gagaaagtgg cggtgattgg
    57181 cgctgggcct gcagggttag ggtgtgctga tattctggcg cgcgcaggag ttcaggtcga
    57241 tgtctttgat cgccatccag aaattggcgg tatgctgact tttggcattc ctcctttcaa
    57301 actcgataaa acggtattaa gccagcggcg agagatattc accgcaatgg gaatcgactt
    57361 tcatcttaac tgtgaaattg gccgcgatat tacctttagc gatttaactt ctgaatatga
    57421 tgcagttttc atcggcgtgg ggacttacgg gatgatgcga gcagatctgc cgcatgaaga
    57481 tgcgcccggt gtcattcagg ctctaccgtt cctgactgcc catacccgcc agctcatggg
    57541 attgccggag tctgaagagt atccgctgac ggacgtggaa ggtaagcgag tcgtggtatt
    57601 gggcggtggc gatacgacaa tggattgttt gcggacttcc attcgcctca atgctgccag
    57661 cgtgacctgc gcgtatcgtc gtgatgaagt cagtatgccg ggctcgcgca aagaggtggt
    57721 caatgcgcgc gaggaaggtg tcgagtttca attcaatgtt cagccgcaat atatcgcttg
    57781 tgacgaagat gggcgtttaa ctgcggtggg cctgattcgt accgccatgg gtgagccggg
    57841 gccggatggt cgccgtcgtc ctcgtccggt tgcgggttca gagtttgaat tgcccgccga
    57901 tgttctcatt atggcctttg gtttccaggc gcatgccatg ccgtggttgc agggcagcgg
    57961 aattaaactc gataaatggg gcctgattca aaccggtgac gtcgggtatt tacctaccca
    58021 gacgcatctg aaaaaagtct ttgctggtgg tgatgcagtt catggcgcgg atctggttgt
    58081 cactgcaatg gccgcaggaa ggcaggcggc gcgcgatatg ttaactctgt ttgatacgaa
    58141 ggcatcgtga tgaaatcgtt aattatcgtt aatccggctg actgtattgg ctgccgtact
    58201 tgtgaagtgg cctgtgtggt cgctcaccct tcataacagg agttgaatgc cgatatcttt
    58261 ttaccccggc tgaaggtaca gcgactggac tgcatcagtg cgccggtgat gtgccatcag
    58321 tgtgaaaacg ccccttgtgt tggcgcttgc cccgtggggg cgctgacgat gggcgagcag
    58381 gtggtgcaga ccaattctgc ccgttgtatt ggctgtcaga gttgcgtcag tgcgtgcccg
    58441 tttggaatga tcaccattca gtcgttgccg ggggataccc ggcaacaaat cgtgaaatgc
    58501 gatctctgtg aacagcggga agaggggcct gcctgcgttg aatcttgccc aacacaggcg
    58561 ctgcagttgc ttactgaaag agaactcagg cgagtccgcc agcagcgtat tgttgccagc
    58621 ggtgagaatc ctctgtgaaa gaaatctccg cgctgaaagg cgcggagaat ctattagatt
    58681 gcccaaccac ccgcgtaaaa tgcgaccagt gcggcagtaa tgatcaccgt accaatattc
    58741 agcttttgcc attcgcgtgc aaagacgcgg cctacgacca gtgtcacaaa gcccagcata
    58801 ataccggtaa cgatattaca agtcagaacg atgaacacgg cacacaccag gccagccata
    58861 gcgtcaataa aatcattgaa atccagcttc gagacgttac ttaacatcag caaacctacg
    58921 tacatcagag cgggtgcagt ggcgtaacca gggatcaaaa atgacagcgg tgataagaac
    58981 agaatcagca ggaataacgc ccccactacg gttgcggtta accctgtttt acccccggcg
    59041 gcggttcctg ccgctgattc gatataaacc gctgcgggcg ctgcgcccac caggccggag
    59101 aatattgaac ttactgagtc actggtcagg gctttgccgc cgttgatgat ctggttgtct
    59161 ttatccaaca aattcgcctg accggcgacg gcacggatgg tgccagtagc gtcgaacact
    59221 gcggtcatca ccaatgccag tacactcgga agtacagttg gctggagtgc gcccataata
    59281 tcgaggctga aaatcagaga cttaccatct tcgccagtca ggcttggcat cgccaccaga
    59341 ccgtggtatt tcaccgctgg gtcaaagatt aagccgatga tcgaaattgc aataatcacc
    59401 aacaagatac cgccgggtac gcgacacttc tccagaccga agatgaccgc cagccccagc
    59461 aagctcatca tcaccgggaa ggaggtaaac gcaccgagcg ccactggcaa gccttcaatc
    59521 gggtttttga taaccatacc cacaccgtta gcagcaatca gcagcagaaa cagcccgata
    59581 ccgatacctg taccgtgagc gatacccatc ggcaaattac gtaagatcca ggtacgcaca
    59641 ccggttacgg aaatggcggt gaagatgacg cccatcagaa ataccgcgcc cagtgcgaca
    59701 ggaacgctaa tttgttgccc gagtaccaga ctgaatgcgg taaacgccgt caaggaaatc
    59761 gcgcaaccaa tcgccattgg caaattagcc cataacccca tcagcaacga gccgaagccc
    59821 gcgaccagac aggtggcaac aaacacagct gcgggaggaa aacctgcttt gcccagcatt
    59881 cccggaacga cgataacgga ataaaccatg gccagaaagg tcgttaagcc agccagtact
    59941 tcctgacgaa cggtactgcc acgagcggta attttaaaat aattatccag cgcgccctgt
    60001 ggctttggtg cgtccggtgt ttgtaggatg tctccagaca taatgatgtc ctcacgagga
    60061 tgtaaaagtt ttattattag ttgcgttcgt acaccagacg accatcaacg taggtgcggt
    60121 agatcgaacg gtcatcgccc aacgtcatca tcacgaacaa tttgtcgact aaagaaacag
    60181 agttgtcata gcgcagctgc tgtagcggag tggcggtggg ttccatcacc acgaaatcag
    60241 cctctttgcc aggtaaaaag ttgccaatca aatcgtcaag gcccagagat ttcgctccgc
    60301 cgagcgtggc caggtaaaac gcttcatatg ccgagaggcg atagccttgt aattgcaaca
    60361 ctttgtaggc ttcgttcagc gtttgcagca tgttgaaagt ggttccggca ccgatatccg
    60421 ttcccatgcc cactttaact ttcttctgcc atgctttttt caagttgaat aacccgctgc
    60481 cgaggtaaag gttggaggtc ggacagaaag caatgctgga tttggtttcg ctgagacgat
    60541 cccactcttt ttctttgaga tggacgcagt gagcaaagac acagttttta ccggtcaggc
    60601 cgtactgatg gtaaacatcc agataaccat catggtcagg ataaagcgat ttcacccagg
    60661 caatttcatc tttgttttca cagagatggg tatgtaccca cgtatccgga tattcctctt
    60721 tcaggcgttg cgccatcgcc atctgttcag gagaagaagt aggggcgaag cgtggcgtaa
    60781 tcgcatatag cagacgacca tttttgtgcc agcgttcaat cagttctttg ctttggtgat
    60841 agctgctttc ggcagtgtct agcagataat ccggtgcgtt gcggtccatc atcaccttac
    60901 cggcaatcat acgcatattg atatgactgg cggcttcaaa cagcgcatca acggattgcg
    60961 gatgaacagt gccaaacacc agcgccgtgg tggttccgtt acgtaaaagc tgcttgatga
    61021 agaacgccga catttcgcgg gcgtactcta aatcctcata acgacgttca gtagggaagg
    61081 tgtgtttatt caaccactcc agcaattgtt ccccataggc ccccaccatt tcactttgcg
    61141 gataatggat atgtgtatcg acaaagcccg gtactatcag tttgccgcga tagtcgcgca
    61201 cgcgaatagt gtcaggaatt tgatgctttc cgtcttccca ttcgccaaac cattccactt
    61261 ttccctgttt aatgagtaat aaaccatcct caataaaccg cagcgcagag gcaatctctt
    61321 ccgggttatc gaccgtacgg gtgacatcaa taaaactgcc tcgtaccgct tttaacgtgt
    61381 gttctcctga catcattgac tcctttactg ttaatccatc tcttccgctg aggtaatacc
    61441 aggcagaacg ttttcctgtc ggtagccacc agggaggata atatttaata aaatcgcagt
    61501 taacccgcca gcacaaatag ggttttcaac taatacataa atagaggctg gcaatatttt
    61561 aaaaatttcg ggatcgtagg agacgccaag tcctaaacct aaagaagtgg cgacaataag
    61621 tgtttctcgg cgctttaaac cgttggtgat gatgatgcga atcccagcga tggcaatcat
    61681 ggaaaacatc aacgtcattg cgcctcccag aactgccgag ggaatggtcg tgaagaagcc
    61741 gccaatcatc ggaaacaaac cgaggataac cagcattacc gcgatggttc gcccgacata
    61801 acgtgaagcg acgccagtca tctgaataac cccattattt tgcgcaaacg tggttaaggg
    61861 taatgacccg acagcggagg cgataacaga aaccagaccg tctgccagca cgccgccttt
    61921 cagtcgggac tgatactctt ctccctgaat gggtcggcgg gaaaccattg ccgtggcggt
    61981 gatatcgccg acagcttcca gcacgctaag cagataaatc gtgccaacca ccaggaactg
    62041 atggaagcta aaactaaagc cgtatttgaa cggatgtggg atggtgatta acggcaaatt
    62101 gcgcatactg ctgaaatcca ccatgcccag gcataacgac gcgatataac cgacgcatag
    62161 cccaatggcg atccctccca tgcgtagcaa cggactgcga cagcagttaa agccgatcac
    62221 cacaatcaaa accaataaac caacgccgag atgttcgtaa ttgccgaagg taccgctgct
    62281 tttggcagca aatcctccac caaaatcgat aatgccgact ttaatcaggc ttaagccgat
    62341 catcagtacc acaataccgc tgacagtagg cgtaataacc cgacgtaaat agggcaagat
    62401 aaatgaagat ccgacaacca gtaatgcgcc aacgaaggag acgccgagga gcgacgacat
    62461 gattaactct tcgtgaaaac cgtcgctttt catgctgctg cccagcgcaa tcatgaccgt
    62521 aacaaatgaa aaattgactg actgaattga gagtaggcca gaaccgacga tgccatagcg
    62581 gtttacttgt aaccaggtac caataccaga ggcgatcatc gccattgaaa caagataggc
    62641 agttgtttca gcggaaagct gtaaggccgc ccccacgatt aacgcggggg ttaccatcgg
    62701 aacgaaaatt gccaacagat gggtaatggc accaacgaga gcctgatgaa agggagggcg
    62761 atcctccagt tcaaatataa ggtcagaacc tgcatggttt atatcagaca tcccttcact
    62821 ccttttaaag taaatttgat catctgaatt agtaaaataa attcccacga atttggggcg
    62881 caggacgcct tgagaacggc ttatctcatt ttcgcagtca ctatttagtc atcgtccaaa
    62941 cagagaaaca ggccgccgaa gcggccattt attatttata tcttttccag cgcggtgagt
    63001 attttctccg gtgtgaaatg ccattcgcgt aaccagatgc cgcatgcatc gtgaattgcg
    63061 gtagcaatcg ccggagctgc gccatttaca ccgatttccg agatcgattt cgccccgaac
    63121 gggccgactt tatcgtcgct cggcaccagc actgcgcgga aatcacgcgg aatgtcacca
    63181 attttcggtg cgccgtagct gcgtaaatca cgcgttaacg ggtgaccttc ggcgtcataa
    63241 atgatctctt cgctcatact gtggccgata gctcgcaggg tagcaccgta gatttgtccc
    63301 aacgctaact ctggattgac cggtgtaccg cagtccagca aggcgtagaa tttatccagg
    63361 cggatttcac ccgtacgcgt gttgacggca acttcagcga agtttgcgcc atacgggaag
    63421 gcgaaatcag gcgtgatata actgccagtt cccaccagtg aaccaaagcc ggtgccggtt
    63481 tcgcctttat gggcaatatc cccgaaacta acttcgcctt tcttgccgcg cacgacgccc
    63541 ggcgttgcta gttgaacatc tgccactggc tcacccaaca tttgcgcgcc gtggaacaga
    63601 attttctccc gcagattttc cgctgccaaa cgcgccgcgt tacccgagaa gcaagtaccg
    63661 gacgaggcat atgcgccttt atcaaacaac gcatgatcgg tatcaccgga gataacatgc
    63721 acgtcctgcg gtgggcagtg cagcacttct gctgccagtt tcgtcaccac ggtatccaga
    63781 ccagtaccaa tatccgcacc gccagaatga acgataaagg taccgtctga ttccagtttg
    63841 atcatgcagt tagcctgatc gatatccggg atccctgatt tctgcatgat aatggcgaca
    63901 ccacgaccga tgtgccagtc accattttgt ggttttggtg aagaccattg gatcatctcg
    63961 cgcccctgac gcaggatctc ttccagtgcg cagctggcgg cggaaggaac ggaggtcggc
    64021 gctttacctt caccgattgc accgagaatt ttcagctctt gcccttcgtg tacccggtta
    64081 cgttcgataa tttccagttg gtcgatctgt aactgttcag ccagttccgc taatgccatg
    64141 gtgatagcga agttaccttt cggtgcgcca taaccctgat aagcaccatt tgggcaaatg
    64201 ttgctgtagt aggtggtgac ctggaaatcg acgttatcgc acggatataa cggcagcgac
    64261 agcgccggtc cgttacacgg tacggtgagt gagtggttgc cgtaagggcc agtgttggcg
    64321 cggaaatcca tcttcactgc cgtcaggcga ccatcttttt tcgctcccag tttgacggtg
    64381 actttcgcga cgtgacgaga ggtgttagca ataaactctt cttcacgggt gtagcggaac
    64441 agtaccggac gcccggtcac gcaggttgcc caggcgcaca cttcttccag caggatgtcc
    64501 tgtttggaac caaaaccgcc gccaactcgc tctttaatga catgaacttt atgctgtttc
    64561 atgtccacga ggcgcgcgac ctggcggcgt aagtgccatg gtacctgggt ggaggcgtgg
    64621 ataaccagac gatcgccgtc catccgggta aagcagatat gtgtttcagt cgggcactgc
    64681 tgcgcctgcg ttgagttata ggttcgctca atgatcacat cggcatcggc aaagcctttg
    64741 tccatatcgc caatatgacc atgaatacta gcggcgatat ttttgcgagg gcgagaaccg
    64801 atcgggaagt tgatgatcat atgctcgccg cgctgggctg catggctgtt atcgtcttcc
    64861 agagtatctg gcgcaccagc aacatacacc accggttcat cgtgcacgac aggcgcatct
    64921 tccgccattg cttcgtcgat cgacattacc ggcttaagca cttcatattc aacctcgatg
    64981 agtttcaatg cttcgagcgc aatttcttca ctttcagcga ccaccgcagc aacgcgatcg
    65041 ccgacgtgac gcattttttt gccgaacata cggcggtcaa gcggtgacgg ttccggtgcg
    65101 ctctgacccc ccggggtata gtagatatca gggcaattca ggtgagtaat aacgtgaacg
    65161 acgcccggta aggcttcagc tttgctgaca tccagatgag taatcagtgc gtgagcgtgt
    65221 gggctacgta acattttaat gacgcaggcg tcagccgtta cgcggtcttc aacatagcag
    65281 ggtttcgcct gcaccatttt cgcggcatca gttttaggat aatgcttgcc aatgacttca
    65341 aggtcgtcac ggaaagtcgg agcgatatca atggtggcct gcggattatt tttacgtgca
    65401 accgccagtt caatgacctg atagtattgc tgccagccag catcgcggct gaacaaacca
    65461 gaaagcgcgt cgtcgatctc ttcgcgggta ggtgcggcga tgcgatcgag aagatcggtg
    65521 ataatcagag ctgcagctgg atcgttataa ccagactgca ccacgccaac atcaaccatt
    65581 gcctgttgaa ccagacttaa ctcgttccat ttgcccagag attctgcggt acgaatatct
    65641 gccttctcta actgtgcggc aataagcaag gacgcgttaa cgatattacc gttaaagatg
    65701 attgcgtcag aaccggcaaa cccgaaacca tcatcactgt tgcgtacaga gtgcattccc
    65761 atgttaaaca acagcttttg cacgttttcg cctggattaa cggttagctc ctgaggcgcg
    65821 ccatttaaag taaagtggat gatcatacgg cttcctcccc agcctgttgg cagtcggcat
    65881 acaggtcggc taccagaact cccgtgatat agcgtttata ggccacgctg ccccgcaaat
    65941 cttcctgcgg gaaaatggcg ttggcgacag cctgttcaag tgcattgcct tccagatttt
    66001 gtttttcgac atcatgcaga cgcagtgctt tactggcgac gccatccagc gcaattcgca
    66061 taccgtcgtg gtctgtcatt gcaacggctg ccgtcacgac ggttaaacct gcctgagaac
    66121 ggctaatttt gcgggtcgca caggtgcgat acggatcttt aatgataatt tcggttagca
    66181 ggcgatcgca tgggcaggcc aggtagtcct cgattgacag cgtttcgccg ttgccaaaaa
    66241 ccagttcagc atccagtgcc agcaggacgg gaagcagcac tgactcttcc tgccgggcgg
    66301 cgatttcacc accaatggtc gactgattac gaacatggcg tgagtaaaca aaaccgaggg
    66361 cttcacgcag tgctgcagga ataaatcgcg catcacgcag tggctgcaag cgagacattg
    66421 cgccaatccg cagtgcaccg ttatcccagt caacccagtc cagttccaga tcctgtaagg
    66481 aaatggcaat ctttttatcg gtacgggttg gtgtagcatt gagtttgctg cccccggcga
    66541 accagacggc ttcatcctgg tagcggcgct tcagttccag cgcctgttcg acagagtcgg
    66601 gcctgaaaaa ttgttcaatc atcttctttc cttatttaac cctcttcctg cgggaagagg
    66661 gcatctgtca tttatgccag cgcatccatc cgacgccaca tactggcagc ggcttttctg
    66721 gcttgtgcat aaatggaatc gcaatcgaag ttaaactgac ggtcttcata gaccatcaca
    66781 ccattgacca tcacgctgtg aacgctgcct gagcccatac cgaaagcgat atgcccggcg
    66841 atattgtctg ccagcagcgg cgtcggcgag ttgtaatcgc aaatggttaa atcagctttg
    66901 taaccggctt ccagaagccc aaatttcgcg ccaaagttgc ggctcatcag ttcgttaccg
    66961 ttagtcaggg ctttggcaaa actgtcaggc cacagcggac cacccgcatc gcgatgttta
    67021 aagaaggcaa atttcatctc ttcaaacatg tccgaaccaa tgccgtccgt tcccaacgcc
    67081 agattgcgga tgtcgctaag gtgatggttg tagccgacat ggttgttcat gtttgaacgg
    67141 gcgttatgca ccaggaacgc atcgcgctga ttgagtaggg cgatgtcatc tttcgacaag
    67201 tacagcccat gagcgaccag cgttttgcta tcgatgagat cgaattgcgc cagtcgtgcc
    67261 agcaggtctt tgccgtacca gtggtgactg taggaaacgt cgtaaaggtc ttccgcagcg
    67321 tgaatatgca aaccacggcc tgtggcgttc acggcttcac gtagcatctc cagaccggca
    67381 tctggcacgg taaacggcgc gtgagcaccg atatgtgctt ccaccagata cggctcggtt
    67441 gctgctttct tcgcctcatc aatctgacgg gcgaagcgga tgttttcttc tacaccttcc
    67501 tgcaactctt tgatgccgtt gttacggtca gtagtttcaa aacaggtcat cgcgcgcagg
    67561 ccaactttta aaaatgcgtc gcgcaatgtg gagagcgacc cgccgatata cgccggagag
    67621 gcatggtgat cgataaccga tgtacatccg ctcttaatcg cttccaggga acaaatcagc
    67681 ccgctgtaat agagcgactc ttcatcaagg gcgcgatcga gccgccacca gagatttttc
    67741 agcgttgaga tgaaatctgg gctgggggcg atgtttgcca taattccgcg ggaaagcccc
    67801 gagtaaaaat ggttgtgcga gcagacaatt cccggcatca caatccgacc atgcatctct
    67861 ttgtagctgg catcggggta gcgttgcgtc agggcatcgc cgatagcgac aatcacatca
    67921 ttttcgatag cgatatcaac gccttcctgc actttcgccg ggtgtaactg cactgcggtg
    67981 acattcttca gaatcaacat gattacacct ccacgcggcc cagcagataa tgatgatgct
    68041 ggtggacatg gctgatgatg cggcacatat cgttcagctc cggtggtacg ttgttgaact
    68101 gaccttcgct gtcgatgttt aacacccagc tttggttatt cagtcgtact cgtacccggc
    68161 aatcttccac aaggaagcct gggttgctgc tgttatcaaa gtcttgcgcc aggctgaaaa
    68221 cggtgatttt gtctttgtat ggtttaccgt tccacggaca gaactgagcg cagttgccgc
    68281 attcgttaca gtaagcgtcg aggtgcagcg tctggaatct attctggaag cctgggaccg
    68341 caatggagac gttggcgcgg ttcgggcaga catccacaca cttgctgcaa acgtagttac
    68401 attcaaggca gcgagcagct tcctgcgcga caaacgcatc acgatcgtca ctgttcacca
    68461 gcgtgataga gatatcgcct ttacgttgat agatttccgc cggattgacg ttgttccagt
    68521 atttatcgtt ctggtgggaa cggatatttt cccgactaag gatggcatcg gtcgcccgac
    68581 gcgcggttcc gacagcagcg acaatggagg atggtccgcg ctgtacgtcg ccgatcataa
    68641 agacgtcagt cagacgagtt tcgccattat ggtcgacgtc tggccagccg tttttgtcca
    68701 gcggcacgcc catcgcattc agggcttcag tatcctgctg ttcaccaatg gcggtgatca
    68761 ggctgtctac atgcagtgtt actgtttcgt tggtttcaac cggacgacga cgacctttct
    68821 catcgggttc gccaagcgac ataacgcgca aggttaaggt gccatcagca tcgaaacgtt
    68881 ccggattatt caggaaacgg aactccacgc cgtcgtgcaa cgcttcttca tactcttcgc
    68941 gccatgcggg catctcttgc agtgaacgac ggtaaacgac cgttgctttt tctacgcctg
    69001 gaacgcgtaa cgccgcacga gcgcagtcca ttgcggtgtt acccgccccg acaacgacca
    69061 catgtttgcc cagcttgagc gctgtaccct tgttgtattc acgcaggaag gggagtgatt
    69121 tcaagacatt ttggttgtcg cccgccagtt tcacaccgct atttttatct gtgccggtgg
    69181 caatcagaac atagtggaag tcctgatttt ttaactgctc aacggttaaa tcgggtgagc
    69241 agccatactc aaatttcacg ccgtgagcgg caacaaaatc gatatcgtgc tgaattaact
    69301 cagccggaat tcggaactga ggaatgatat ttttcaccac gccgcccgca ttggcttcgc
    69361 gttcaaacag cgtaaccgga tggcccgctc tggcaaggaa gtaacctgct gccagacccg
    69421 ccggacctgc accaatcacg gcaaccggat ggcgtgaacc agaaccggct ggtttgtgcc
    69481 agcgttgctt atattcatcc caaccttttt ccagcgcgac ttttttcagt tcgcggatat
    69541 tcagcgcact gtcgtaatcc aggcgggtac agttgtattg gcactggtga tcgcaaatat
    69601 gaccggtaat ggcgggcaga gcgttgcgtt ggtagatgag ttccagcgcg tcggcatagc
    69661 ggtgttcgcc aagcagacgg atgtattccg gaatatcttg cttgatagcg caggcagtaa
    69721 cacagggggc aacgtagcag tcggtcagcg gcaggtcttc tgccacttca atacgctctt
    69781 ctggcttcca gtgtttctgg gtgtattcca tggttaacgc atctgctgcc agtctgttca
    69841 gtcgttcgac gtcaacatgg tcaagtcccc aggcgtcgga gccttccagc tcgcgcatgc
    69901 aggcacttaa gcgcaaatag ccgccaggtt tcagcaggtc ggttgccata gtaatagggc
    69961 gaatacctgt atcaaaaata tcgcggatag tcagctgact ggcaccaccg gaataagaaa
    70021 tgggcagttt gccgtcaaag gcgcgagaga gaactgctgc aacattgatg gagagcggga
    70081 acagcgcacg gcctgacata tacatctctt caccaggcag tgcgccttta ttgttgatgg
    70141 tgccgagagt gttagtcagt tttacgccaa agccgagtga tttttctttt gccagtgcca
    70201 tcaggcgttc cagcatttcc agtgcttgcg tcagcttgag gtcgtgatca aatgactctt
    70261 cttttaagcc tatgtaaccg aaaccgcaga catcgaggat ctcacgaaca cgcgcgtacc
    70321 ccagtaaggt cgggttaagt ttcacaaagg tgttgagccc tttttcttcc agcatgtagc
    70381 ggcaaatggc ttcgatttca tgcggaggac agccgtgcat ggtggagagg gtgacgccat
    70441 gcaccatact ggtggggatg cgagcgggta aggcttgcaa gctttcgcgt ttttcctgca
    70501 atccgtggcg ggctaaaaat gcgtcatcct ggagtaattt attcagcgta tcgcgatatt
    70561 gcgcgaattt cggatggtca gatgcgtcca tcatattgtc gatgaactgt tgcattggcg
    70621 gttgcttaat accttcgagg ttgtaaccga cgctcatatt aaagatgaac gatttaccgg
    70681 aatcagaagg ttggaacatc gcttcgagaa ggtgcagggc aaaccaggct ttgaggtatt
    70741 catcccaggc tttaagcagg gtaaattcgg tagaccattc ggtgttaaag cactcgtctt
    70801 cggcatcaat acagggcttt tccagctcca ggcggtcaag aatttggacg gtttttagtt
    70861 cgatgaaacg tccgccagtc agccaggaag tgacaatatt ttgcgcgagc tgcgtgtgcg
    70921 ggccagcggc agggccgacg ggagtggcac aggtttcacc gaagacgcta acagttttac
    70981 cttttacagg tgagtaaaac tgttgctcgg gaataccaaa gattgagcgt tgttgttggt
    71041 attcatcaaa tatgcgcgtc aaaagttcct caaacggaat gggacgcata atatccccca
    71101 taaccctctc cttagcttat acagatatca ttgagtgacc ggtgttgaga aatccggtgg
    71161 tttaggggga tagagcaaca atcgcaccac tcttgccagg tttggcggga aatataaaaa
    71221 aatgtgaggc ggctcgaaac ttaagggctt tttgtgaatg aactcacagg tgctaaatga
    71281 gaattaaaat cactcctgaa aattattatt gccatcccat atatcacttt ttctcttttc
    71341 tcaaaatcag tgattcctta tcattatgat aaagagtgct ggagggatta aatatttgaa
    71401 cgcaatcgtt taactacgtt ttttaaattt tagtatacct tttttgcgct gtaataaagt
    71461 cgtccggagt atcaatatct aaaattattt ctgcattttc tacttcgacg gaataatgag
    71521 ctcccataag gagtgcctga cgcatattgg tgatattggg gcgcttgatt gcctgcatca
    71581 ggcatggttt tgacactaaa atcggatggc cggggatgcc attatggagt ggcagtattg
    71641 cgccatcatt tcgtaacgac cagatttttc tgaaaatatc gatggtgagg gttggcatgt
    71701 caccgtgggt gagaaaacaa tgttctgttt gcactgcggg tactgcggcc tttactgatg
    71761 ttagtaaacc ctgcgcataa tctgggttgt ggataatagt gatattgctc tggttcgcat
    71821 aacgttcgtg cagttcatta ccacgatagc cggtgactaa aataattcgg ctacaaaact
    71881 gcaacgcatt tttgatactt gtatcaagaa ttgtcccctg ttgccagggt aacatcattt
    71941 tccattgccc cattcttgat gataatccgg cagcggtaat tatacagtcg atggctgaca
    72001 tagtggttcc attatttata ggggctaata acgcctgata attgcgttac ttcctgatgg
    72061 attaagttgg ggataattta tcccagagag gtcataaaga ctcgtgaaaa gtatagttga
    72121 cccatcggca ttattcattg atttaggtgc gcagaaacgt cccactgtga tttctgttgt
    72181 tggtgctggg gggaaaacca gcttgctttt ttggctggca gaactgctcc aggctagcgg
    72241 caggcgtgtg ttaatcacga cgacaacaca tatgtttatg ccaacgtctc actggcccgt
    72301 ggttttctgt cgtgatcccg ccatgcttcc tcatgcgtct cttacatccc ccatttcatt
    72361 ttgttttcac agctggaaag cgaacctggg aaaagtgcag ggatttacgc cagaagcgat
    72421 tgatgcactg gtgcaacgac cagagtgtga cgtaattctc attgaagcag atggctcgcg
    72481 tgcaatgccg ttaaaagcgc ctgatgagca cgaaccttgc atacccaaaa gcagttgctg
    72541 cgtgattgct gtgatgggag gacatatttt gggcgcgaaa gtgagcacgg aaaatgtcca
    72601 tcgctggtcg cagtttgctg atattaccgg gttaacacct gatgcaacct tgcaactgag
    72661 cgatctcgtt gcgctggttc gccaccctca gggggcgttt aaaaacgtac cgcaaggttg
    72721 tcggcgagtc tggttcatta accgtttttc tcaatgtgag aatgcgattg cgcaaagcga
    72781 gctccttcaa ccgctgcaac aacacgacgt agaggcaatc tggctgggcg atatacaaga
    72841 acatcctgca atcgcgcgca gatttgtgaa ttagcggccc gggtattccg ggttactgaa
    72901 aggatatcca ttgaggttgg tatgaatatt ttcacagagg ctgcaaaact cgaagagcaa
    72961 aattgtccgt ttgctatggc gcaaattatt gatagccgag gctcgactcc ccgccattct
    73021 gcacaaatgt tagtgcgcgc cgatggctct atcgtcggta caattggtgg cggaatggtt
    73081 gagcggaagg tgattgaaga gtcgcttcag gcattgcagg aacgtaagcc gcgattattc
    73141 catggacgta tggctcgtaa cggtgcggat gctgtcgggt cagattgtgg aggtgcgatg
    73201 tcagtgttta tcagcgtcca tggtatgcgt ccacgtctgg tgttgattgg cgcggggcat
    73261 gtcaaccggg cgatagccca gagtgcggcg ctattaggat ttgatattgc cgttgccgat
    73321 atttatcgcg aaagcctcaa tcctgaacta ttccctccat caaccacgct tctccatgct
    73381 gagtcgtttg gtgcggcagt ggaagcactg gatattcgcc ctgataattt tgtcctgatt
    73441 gccacgaata atcaggatcg tgaagccctc gacaaactca ttgaacagcc cattgcatgg
    73501 ttggggttgc tggcaagtcg tcgcaaggtt cagcttttcc tgcgtcaatt gcgtgagaaa
    73561 ggcgtggctg aagaacatat tgcccgttta catgcgcccg ttggttacaa cataggtgcg
    73621 gaaacgccgc aggagatcgc catcagcgtg ctggcagaaa tattacaggt gaaaaataac
    73681 gcgccgggtg ggctgatgat gaaaccttct catccttccg gacaccagct ggtggtgatt
    73741 cgcggtgcgg gagatatcgc cagtggtgtg gcgctacgtc tgtatcatgc gggttttaaa
    73801 gtgatcatgc tggaagtgga aaaaccgaca gtgattcgtt gtaccgtggc gtttgcccag
    73861 gccgtgttcg atggcgaaat gacggtcgaa ggcgtcactg ctcgcctggc aaccagctct
    73921 gcggaagcga tgaaacttac cgaacgcgga ttcatccctg tgatggtaga tcccgcctgt
    73981 tcattgcttg atgaactgaa accgctttgc gtggtggacg ctattctggc gaaacagaat
    74041 ttgggaacac gggcagatat ggcaccagta acaatcgcgc ttgggccggg ctttgctgca
    74101 gggaaggatt gtcatgcggt aattgaaaca aatcgcgggc actggctcgg tcaggtgatt
    74161 tactgtggtt gtgcgcagga gaataccggt gttcctggca atattatggg gcataccacc
    74221 cgacgggtga tccgtgctcc tgctgcaggc attatgcgat ccaacgtgaa attaggcgat
    74281 ctggtgaaag agggcgatgt gattgcctgg attggtgagc atgaaattaa agcaccgttg
    74341 acggggatgg tgcgtggctt gttgaacgac ggcctggcag tggttggtgg ttttaaaatt
    74401 ggtgatatcg atcctcgtgg tgaaaccgct gatttcacca gcgtttctga taaagcccgg
    74461 gcgattggcg gcggcgtact tgaggcgtta atgatgttga tgcatcaggg cgtgaaagcg
    74521 acaaaagaag tgctggaagt ggcttaaatg aaaatggccg gatggtatta cccacccggc
    74581 cttaaaatta ctgacaaatc accgttccgg ttttgccttc aatcccttct ttcgctttgc
    74641 tcaatacggt aatcagtgct tcacgacctg cgcgagagcg agcaaacgag gcggcagctt
    74701 ccactttagg cagcatagaa cctttcgcaa aatgaccttc ttcgataaag cgttcagcat
    74761 cacttaatga caggcgatcg agccactgtt cattctcttt accgaagtta atggccactt
    74821 tttctaccgc agtcagaatg atcagcatat cggcatcaat catttctgct aaacgggcgc
    74881 tggcccagtc tttatcgata accgcgctgg caccacgcag atggttacct tcacgaataa
    74941 caggaatacc accaccgcca acggtgatca ccacctggcc tgcttctacc agcgctttta
    75001 ccgtctcttt ttcaatgata tcaaccggtt ttggcgaggc aacaacacgg cgatagccgc
    75061 gaccagcatc ttctttcaga gtgtaaccct gttttgtcag ttgctctgct tcttgctcgg
    75121 taaagaacga accgatcggc ttggtcgggt tgaggaatgc cggatcgtta gcgtcgactt
    75181 cgacctgagt aaccagcgtt actacaggtt tattaatgcc ccgagaaagc agttcttctc
    75241 ttaaggcgtt ttgtagatcg taaccaatat aaccctggct taaggcaaca cagacagaca
    75301 tcggcagcat tggcgaatgc gcttcagttt ttgcggcagc ttcaaaggcc tggttaatca
    75361 tgcccacctg cggcccgtta ccatgagtga cgataacttc atgtccctgg gcaattaaat
    75421 caacaattgc ctgagaagta atttttaccg ctttcatttg tccggccagg tcatcgccca
    75481 gcgcattccc gcccagggcg agaacaattt tcttactcat ttttcctcac tcatcattct
    75541 tgattgtgta tagatccctg gcagtggtta gagcacggga gggacaaacg gtttgcgtcg
    75601 caggaaacga cctcgcccgg ctttgcctgt aaaggtgccg tcacagaaaa tcgtttcacc
    75661 acgggataag gttctgacaa tcgcgccctg acaggtaaaa ccctcccatg gcgagtagtc
    75721 ggcgttgtcg tggagatggc gatgctgaat ttgttggctc tgacgtgggt cgataatcac
    75781 cacgtcgcca tcggaaccgg gcgctaataa tcctttttgc ggccacaggc caaacagcct
    75841 ggcgggcatt gcgctggtta attcaacaaa gcgttccggt gttatacgtc ccgtcatcac
    75901 gccactggaa aacagtaact gcatgcggtt ttccacaccg ggtaagccat ttgggcagcg
    75961 actgaaatcg cctttagaaa tttgcaggcg ttgagccatc gagaaggtgc agtgatcggt
    76021 cgccaccacg tcaatcgcac catcgctgat gccacaccac agtttgtcct gctcgcgtac
    76081 gttacgcagc ggtgggctaa gaatgaactt catgccgtct tctgtatcgt aactgcgttc
    76141 gtccaacagg agatattgtg ggcaggtttc aacccagact ggctggtgat tcgcacgggc
    76201 aagacgcaga taatccagac ctaagccgtt agacaggtgc acgatataga gcggggcgtt
    76261 accggcaatt tgtgccaggt taatcatgcg ggcgatggct tccgcttcgc attccagagg
    76321 gcgactcaag gcatgatagc gcggcgcggt taaccctgcg gcgataaatt ccgcccgctt
    76381 gctggcgata gccgcatcat tttccgggtg cacggtggtc agcgcgccgg attcatgcag
    76441 acggcgtaat gcctgcaaaa cctcgtcatc gttgagtttg tattgatagg ttaaatagag
    76501 tttaaaactg ctcagccctt cctcgaccat catcgggatt tcgtcgagga tggcgtgatt
    76561 aatgtgctgg atcacaccgt gaaagctgta atcgatgacc gctttatggg cggcataacc
    76621 acgataaacc tccagttgat ggcgtaaccg acagccgttt gggccaaatc ccatatggtc
    76681 aataatggtt gttgtaccgc cacacgcagc tgcgcgggta ccggtaaaaa aatcatcaca
    76741 actgcgcgcg atgccgacat caatattgaa atgcgtatgg acatccacgc cgccagggaa
    76801 aacgtaacag ccagtggcat caatttcttc atacgggagc tgcggcgaaa tattgttgcc
    76861 caactggcga acaatcccgc tttcaatcag caaatcctgt ttggcttgtc catctgcgtt
    76921 aacgacagtg ccgtttttga tcaatacgcg catagcaaac tccaaaaacc ctccggcgaa
    76981 ccggagggga ataagtgaag agtaattatt cggttgccag ccagcttaac gggattgcag
    77041 cgtacatcgc ggcacaggtc accaggtgag atttccaggt tttttcgtta ggtgcatgcg
    77101 cttcaggttc tttacccggg ccaaagccga tcaccggaat gccgtgacgg cccatgatag
    77161 atacgccgtt agttgagaag gtccacttat caacaaccgg cgctttgcca aacagccctt
    77221 cgtaggcatt caccagtgct ttaacggtga agtgatcttc ttccactttc caggtcggga
    77281 agtagcattc ggttgggtaa accaggccag tccaggacgg acggtcgtag ttgtacatag
    77341 aaacaaccgc gttagctttc tgtactgcag gcagggcgcg gatttcgtcc agcgcgcctt
    77401 cccaggtttc gccccaggtc agacggcggt caatagagac ggcgcagcta tctgctacag
    77461 cgcaacggct tggggatgtg aagaagattt cagaaacggt gagggtgcct ttgccgagga
    77521 attcgtcata acccagacgt tgggagagtt cttgtaattc gccaagaatc ggacccattt
    77581 tgaaaatggc gttgtcaccg cgttctggcg cagaaccgtg gcagctaaca ccctgaacat
    77641 caatacgaat ttccatacga ccgcgctgac cacggtatac ctggcagtcg gttggttcgg
    77701 tactgaccac aaattccggg cgaatgccgg attgttcaat aatgtactgc cagcacagac
    77761 cgtcgcagtc ttcttcctgc acagtaccgg taaccagcag ggtatattca tcttccagac
    77821 cgaggtcttt aatgatttta ccggcataaa ccatagatgc catgccgcct tcctggtcgg
    77881 aagtaccgcg accaccgatc agctcatcag tttccatgcc ttcgtacgga tcgaagtccc
    77941 agtttttgat gttgccaatg ccgacggtat cgatatgagc gtccattgcc accagacgcg
    78001 ggccgtggcc gatataaccg agaacgttac ccatcgggtc gatttcaact ttatcgaagc
    78061 cgactttttc catctcttct ttaatacgat gtactacccg tttctcgtcg cagctttcac
    78121 tgggaatagc aaccatgtcg cgcaggaagc gagtcatatc cgcctggtaa tcttttgctt
    78181 tttcaagaat cagtttgaat ggaatattct tagccattat ttttctccag attttttact
    78241 ccccgttttg cagggagcag ttccaatagg gtgattaagg tgctacagcg tgtttgcctt
    78301 cccagacaac ttcgcggtag tgcttcacgt cggtgtcgcc ttcagtgctg ataaccagca
    78361 ctacggcatc tttgttcagc gccagttttt ccatcaggct ttgacgttgc gggtgataat
    78421 gaaccgctgc gagaacgccc aaaccgacag cgccggattc accggagatg atgcgcgggt
    78481 cgttgccgta cggattaccc agcacgcgca tacctaatgc ggcaacgctg tcctggcagg
    78541 agatgaattg ggtggcgcag ttacgtagga tttcccagcc cagcgggtta ggttcgccac
    78601 aggccaggcc tgccatgatg gtggccatat caccgccaac gttgacgatg tcgcctttga
    78661 cgccggagcg ataaatacag tcagctttgt caggttcaac aataatgctg tgcagatttt
    78721 gcgggctata gacgtcgacc agataaccca gcacaccacc ggccattgct ccgacaccgg
    78781 cttgcagcag aacgtgcgtc ggggttacgc ccatttcacg catttgctcg acggcttcat
    78841 ctgccagggt tgcgtagcct tgcatgatcc aggttgggat tttggtgtaa ccttcccatg
    78901 ccgtgtcctg taccacttcc cagccgtgct gctgcgcgtg ttgcatggtc aggcgaacgg
    78961 tatcgtcata gttcatatct gtgacgatgc actcggcacc gaggttcaga atggcgtcaa
    79021 cgcgttcctg agcagaacct ttcggcatgt aaatcaccgc attctgtccg agttgctgcg
    79081 ctgcccacgc cacaccgcgc ccgtggttgc cgtcggtggt ggtcgcgaaa gtcatttttt
    79141 cgccgatggc atttttcagg tgctcaaatg acagcgtttc gatatcaaga tgatattttt
    79201 cacacaataa ttgagcgata gcgtacgcac cgccgagcat tttgaaggcg ttcagaccga
    79261 atcgttttga ttcgtctttg acaagaattt tcttcacccc aaaaaggttt gcgagatcgt
    79321 ccagcgcaca aagcggtgtt gggcgataac cagctatttt ctggtggaac tggcgcgcca
    79381 gtttggcctg gctttgcgaa aagagcggtg atgtttcgcc gttgaaaaat ttgttatcgg
    79441 cgatatcaat cttcaatgag aaaacggaca tatcctatcc ttttggcctc tggaaataga
    79501 gggccgacca gctgatcggc ccgaggggtt atttcacgcg ttcttgcgct tctttcagca
    79561 gttgctcgag cagtgcacct ggtttggcgt atttacggga caggatcatc gcggcgatga
    79621 tataaggctt ccagctggct tctttgtagg tagcgatacg gtatttttcg aatacgcctt
    79681 cagtcacttc cccttctttg caggatacgc cgctgatatc agctggcagg cagtgcatgt
    79741 acagggcttc gccatcacgg gtcagttcca tcatctcttc agtacaatgc cagtctttgt
    79801 gttgcgcgtt ctgtgccaga cactgttttt ccagtgcttt taagccttcg tgatcgttag
    79861 cgcgcagcaa ttcggtacgc tgttccatca ctttgtaagg tgcccatgac ttcggataaa
    79921 cgatgtctgc atctttgaac gcttcttcca tgctggtgac ctgacggaag ctaccgccag
    79981 atgctttagc attgtttttc gcgacttcta ctacatccgg gatcaggtcg tagccttccg
    80041 gatgggccag ggtgacatcc ataccgaagc gagtcatcag accgatgatg ccttgtggta
    80101 cagagagcgg tttgccatag cttggagagt aggcccaggt catggcgatt tttttacctt
    80161 tcaggttttc cagtgaacca aagtgttcac gtaaccatgc caggtcagcc attgactgag
    80221 tcgggtggtc aatatcgcat tgcaggttca ctaaagccgg acgctgtggc agtacaccct
    80281 gcttgtaacc gtcatcaagt gcagcgccaa cttcacgcat ataggcgttg cctgcgccca
    80341 gatacatatc gtcgcgaata ccaatagcgt cggcgcagaa ggagatcata ttggcggttt
    80401 cacgcacggt ttcgccgtga gcgatttgtg atttgccttc atcgagatct tgctgtgcaa
    80461 ggccgagcag gttaagcgcg gaagcataag agaagcgggt acgagtggag ttgtcgcgga
    80521 atacggaaat acctaatcca ctattaaaga ctttggttga gatgttttca gcacgcagtg
    80581 cttttaatgc tgcggcaacg tccagtactt gtttcagttc atctggagtc tgttcccacg
    80641 ttaacaaaaa atctttctcg tgaaggtgag aggtcagcga attgatatcc ttaatcagct
    80701 cattaacagt tttcatcata aagtcccggt agtggaatga caggcaaagt ggtttatttc
    80761 actgtgactt gccaaagtga ttggtgtttt cacagctata cctgggacta acaataagtg
    80821 tgccagaatt atccacgggg ctgcaaaaga ctaatttgac ggataatgtg tggggttgat
    80881 cacaaattga tgccagcttc cggttgtttg taaaatacga aaacgtttgg ctttttttat
    80941 cgcgttattt atttgctgct aagtatcaga aataagataa agggacaggc tatccctcgt
    81001 cagtttgata aactcaccgc cataattctc atattgatat agaaatgtga tatctcttcc
    81061 ataatttcat tagaaagtga taaaaatctt atgtttttat caaagccaat ttcgcctgag
    81121 ttaatatgat catgatctgt gaaccatcaa cgtcttcaat gatcgacttt taagtaagtc
    81181 tggaaggtaa agctatggag cttgctacta cgcagtcagt attgatgcaa attcaaccga
    81241 caattcagcg ttttgccaga atgcttgcca gcgttttgca gcttgaggtt gagatcgttg
    81301 atgaaaactt gtatcgcgtc gccggaacgg gcgcgtatgg gaagtttctt ggtcgccagt
    81361 tgagcggcaa ctcacgcctg ctctgccacg tcctggaaac gaaaactgaa aaagttgtga
    81421 cacagtctcg cttcgatccc ctttgcgaag gttgcgatag taaagaaaat tgccgcgaaa
    81481 aagcatttct gggtacgcct gtcattttac aggatcgttg tgttggggtg ataagtttga
    81541 ttgccgttac ccacgagcaa caagagcata tcagtgataa tttacgcgaa ttttctgatt
    81601 atgttcgcca tatatccacc atttttgttt cgaaacttct ggaggatcag gggccaggag
    81661 ataacatcag taaaatattc gcgaccatga tcgataatat ggatcagggc gtattagttg
    81721 ttgatgatga aagtcgggtt cagtttgtta atcagactgc cttaaaaaca cttggtgttg
    81781 tgcaaaataa tattattggg aaacctatcc gtttcagacc attaacattt gagagtaatt
    81841 ttacccatgg acatatgcag catattgttt cgtgggacga taaaagtgaa ttaatcattg
    81901 gtcaattgca taacattcag ggccgacaat tatttttaat ggcgtttcac caatcgcata
    81961 ccagtttttc tgtagcaaat gcacctgatg aaccgcatat tgaacaattg gttggcgagt
    82021 gccgtgttat gcggcaatta aaacgactca ttagccgtat tgcacccagc ccatccagcg
    82081 ttatggtggt tggtgaaagc ggcacgggta aagaagttgt cgcccgtgca atccataagt
    82141 tgagcggaag acggaataaa ccctttattg ctatcaactg tgccgcgatt ccggagcagc
    82201 ttctggagag cgaactgttc ggttatgtta aaggcgcatt tactggcgct tctgccaacg
    82261 gtaaaacagg gttgattcag gcggcgaata cgggcacgct gtttctcgat gaaataggtg
    82321 atatgccatt aatgttgcag gctaaattac tgcgcgctat tgaggcacgt gaaattctgc
    82381 cgattggtgc cagtagccca atacaagtcg acattcgcat catttctgca actaaccaga
    82441 atttggccca gttcattgcc gaaggtaaat tccgcgaaga tctcttctac cgacttaatg
    82501 ttatcccgat aactctgcca ccgctgcgtg aacgtcagga agatattgaa ctactggtgc
    82561 attacttttt acatctgcat acccgtcgtc tgggatcggt ttatcctggc attgctcccg
    82621 atgtcgtcga aatattgcgt aagcatcgtt ggcccggaaa cctgcgcgag ttaagcaatt
    82681 tgatggaata tctggttaac gttgttcctt caggtgaagt tatcgacagc acgctattgc
    82741 cgccaaatct gctgaataat ggcacaacgg agcaaagtga tgtaacagag gttactgagg
    82801 cacacctgtc actcgatgat gcgggcggca cggcgctgga ggagatggaa aagcaaatga
    82861 tccgcgaggc gctttcacgt cataacagca agaagcaagt tgctgatgaa ctgggcatcg
    82921 gcattgctac gctctatcgc aagattaaga aatatgagtt gttaaacaca taacatcagt
    82981 gaatccggcc tgaattcagg ccggatatcc ttttacttcg ttttctcgca atccagaact
    83041 gtatttacaa tcatctgata ccccgtgcag cgacaaagat ttcccgccag tccgcgacga
    83101 atttccgtaa tggttaatgg cttctcgcgt ggtttcgcca gcattgccgt ggtagccata
    83161 atcaggccag gcgtacaaaa cccgcactgc actgcgccgg atttcgcata agcctgctga
    83221 acatgagaaa gttttccgcc tttcgcttca ccttccagcg tgcggatctc ttttccttca
    83281 gcccaggcgg caaggtataa gcaactgtct attgctgtgc cgtcgaccag caccgtacag
    83341 gcaccacatt cacccacgca gcacccttgt ttgacactta gcagcccttg ttcgcggagt
    83401 aattccgaga gcggcgtgcc tggtacggca tgaagctgaa aaggcatccc gttaatggtg
    83461 cattcgatgg taattgtttc gctgtgattc attgcaattt tccccccgcc gcggcgacgg
    83521 cttcgctaat cacttttttg gtcatcgtct ggatgagatg cagacgaaac tctttactgg
    83581 cccgccatga agaacgcggg gcgacatctt gcaggacaga ttcgctgata gcttccagcg
    83641 tttgcaggtt taatggcgca ttttgtgcag tctgttcggc atgttggcag cgaatcggcg
    83701 ttggcgcggc aacaccaaat gccaggcgta attcgctgaa attgccgtta tccagtcggc
    83761 aatgtgcggc acagccaatc gttgaaatat ccattgcgtc gcgcatggca tatttaaaat
    83821 gcgcgctgcc cacgtgttct ttcggctggg gcggaaaatg aaaggcgaca aggatttcgt
    83881 catgctcaag agacactttg cccggcccgg tgtgaaagcc attaatcggg acgaaacgaa
    83941 caccgcgtgg ggagtggatc tccagtttcg cgtcataaat gagcgttggc gtggcagaat
    84001 ctgcgctggt ggcaccgttg caaatatttc caccgtaggt agcgacgtta cggatctgcg
    84061 gtccagcaat ggacgaggcc gcagcacata acgccgggag atgacgttga gttatagaat
    84121 cttctattag ctgggtaaat gtcgttgcag agccgatacg tagcgagcca tcttccgcca
    84181 gcgtaattcc ccgcagctcc gccagattat gaatatcaac aatatggcga taacggtcat
    84241 tgtgatggtg gagctgaatc agtacgtcag tgccaccggc gagcagtttg gcctgcgggt
    84301 tgtcagccag caggttgatg gcatcggcaa gggttgctgc gcgatggtaa gaagcaaaat
    84361 caaacatgat gttatcctca aatcaatcct gccagatgga actcttcata taaccgtttt
    84421 ggcgtcagcg gcagtgtatt gattgcaaca ccggtagcca tcttcaccgc gttacgaata
    84481 gcagcggcaa caggaattat tggtggctca cccagtgact tatgtccgta tgcggattgc
    84541 ggctcattga tttcgacgaa cgcgctttcc agttgtggca gatccggcat ggtcggcatt
    84601 ttgtaatcca gcagattggg gttacggacc acgccgcttt tcgcatcgat gatcatctct
    84661 tcaaatagcg cccagccaat gcccattccc attccgccgt gtacctgacc ttctgccagt
    84721 agcggattaa gaatatggcc cgaatcatga acgttgagga tgcggttgat ggtgactttg
    84781 cacagcgcaa tatcgaccgt cagatcaaca aaggtacagc cgaacgccgg tgggttagtg
    84841 gtggttttga tggagctttc agcagagagc tgcccgccgc gttcagggtg gtagaaagcg
    84901 tccatcgcca aatcttttaa cgacattaac ggctcttccg gtcgttcaac cagcacgata
    84961 tggcctttta tcagggtcag attcatcgct gactgatgta gcatgactgc ggcgtgagcg
    85021 atgattttct cttttaataa cagtgccgca ctgcgcagcg caggcgcggc aacatagctc
    85081 tggcgtgagg caaatgcgcc gggatcgaac ggcgtaacgt cggtatcttg tgttgaaata
    85141 acgcgaacgt cgctgaccgg aacccccacg gtttctgcca ccatttgcga gaagacggtg
    85201 tcggcaccct gaccgatttc cgtcgcgccg ctttgcacgt tgatggttcc atcctgattc
    85261 atcagaaggc gcgcgcctgc tatttctacg ccgacaggcc aggtgttaga ggtgtagcta
    85321 aaacaggcga cgccaacgcc gcggcgtaaa ttgccttgct ggttctggca ctctgcacgg
    85381 cgtttttccc attcaaagat tttccggcct ttttcaagac actccggcaa ccctgcgctg
    85441 taaatacgtt tgcccgtgag cggattagca tctccttcgc gtgaggcgtt gcgtaaacga
    85501 atttcaacag gatcaatacc taacgctgtc gcggcgtcat caagcataga ctcaacggca
    85561 aatacgactt gtggcgcgcc ataaccacgc atcgcaccag ccgaggggag gttggtatag
    85621 caggtctttg aactgtaagc gtaggcacaa cgaggataaa ggtaagcgac tttatttccc
    85681 cctgcagaag cgatggagtg cccgtgagat acataagcgc cggtgttaga cagaacatcc
    85741 agactataac ctttcaatgt tccgtcgcgg ttcacgccca tttgcccgtc aatagtaaaa
    85801 gcgtggcggg tacgggttgc gaggaaacac tcttcacggc taagggaaac tttcaccgga
    85861 atgccgccaa gcttgctggt caggaatgcc gccattggct cttccagtac atcctgttta
    85921 ttaccaaaac cgccaccgac aaatggtttg atgactcgta cgcatgacca gggaatatcc
    85981 agcgcctgac caaccacgcg gcgaacaatg tgcgggatct gggtgctgga aacgatggta
    86041 attcgcgagt catcctccat ccacgccagc gatgttacgc tttccatgtg acaatgttga
    86101 ataacggggg tctgatagtg cccctgtacc tggtagtcgg cggcatcgat tgtttgttgg
    86161 acattgcccg tcgacatcgt gctttgtttc agtaaattgc cgccgttatg gattggtgca
    86221 gcgtcctctg ccagcgccgc ttctggcgtg gtgataacgg gtaattcttc ccactcaatg
    86281 ctgaccaatt gcgccgcttt ttctgccgtg agttcatcgc gggccacgac gatggcaacg
    86341 gcgtcaccat gatgacgaac atggcgagtt agcagtgcgc gatcggcggt atcgcgcttg
    86401 ttttcgtcaa gtgtccatgc atgcccagct gtagcgaatg ggatatcagg cacatcttcc
    86461 caggtaaaaa tcgccagtac gcctggtaaa cttctggctt gttcatcatt aatacttacg
    86521 gcataaccat gtgcgatagg gctacgtaca tatttcgcat aacacatgcc cgccataaca
    86581 taatcgtcag tatatcgtgc ccgcccggtg accttagcaa tggcatcaac gcgcatgcat
    86641 gattcacccg tagcggttgc ttcccgcgct tccatagaaa tcccctcgat ttgaacattt
    86701 ggttcttatg gcaaagattt agtctaagca ggcaagattt acgccagaaa cggcgataag
    86761 cgttaaggga ggaaggaagt gtgatgatgt taaaaaaaca cagatatgat acgaaacagg
    86821 accctgaata ggggtagtga tacgtatcaa tatgagtggc gataaaatca atgtgataaa
    86881 ttaggattat tacgcatcag gatgggaaat agggtaaaaa agtcaggaac ttcgatacct
    86941 ctacagtgaa tattttgctg atgatacttc cctttgccaa atgaaattaa cctctttctc
    87001 tattacatcg tttttccaga agcaagagat tgggtatcaa gaggctggct gctatgatat
    87061 ggcgtcttgt tttttaagcg aggaaagatt ttgagtgcgg gacgcctgaa taaaaaatct
    87121 ctgggtatcg tgatgttgtt atcggttgga ctgcttttgg cgggctgttc gggtagcaaa
    87181 tcatccgata caggaacgta ttccggctcc gtttacaccg tgaaacgggg ggatacgcta
    87241 tatcgtattt cgcgcaccac gggaaccagc gtaaaagagc tggcgcgact gaacggcatt
    87301 tccccccctt acaccattga agttggtcag aaactaaaac tgggtggggc gaaaagtagc
    87361 agtagtacac gtaaatcaac cgccaaatca acgaccaaaa ccgcatcggt tacaccgtca
    87421 tcagcggtac cgaaatcttc ctggccgcca gtagggcaac gttgttggtt atggccaacg
    87481 acagggaaag ttatcatgcc gtattcgaca gcagatggcg gcaataaagg gattgatatc
    87541 tcagctccac ggggtacacc tatttacgcc gcgggtgcag gaaaggtggt gtatgtgggc
    87601 aaccagctgc gtggctacgg taatctcatc atgattaaac acagtgaaga ttacattacg
    87661 gcttacgccc ataatgacac gatgctggta aataatgggc aaagcgtgaa ggctgggcaa
    87721 aaaatcgcca ccatggggag cacggatgcg gcatctgttc gcctgcattt ccagattcgt
    87781 taccgtgcaa cggcaattga tccgctacgt tacttgccgc ctcagggcag caagccaaaa
    87841 tgctgatggc gaattaatca gcagtcagca gcgcggcact tgctaagaag agcgtaaggt
    87901 ttataatgcc ttacgcatct cgaagcgggc gtagttcaat ggtagaacga gagcttccca
    87961 agctctatac gagggttcga ttcccttcgc ccgctccaat ttatcttcac ctcatcaatc
    88021 tgccattttt gctgtatctg attttctata ttatcttaat tcaatcgctt cagacttttc
    88081 gcaatctctg taacactccc ccattttttt gtaaataaaa agcgtttacg caatgcaatc
    88141 cttttgtaac aacccaggta aaatacctta ttttcaagtt gttaaataag tggttatcta
    88201 cgcttttaac aaacgattga tggaatattt tatgaaaggg aaatctgcat taactcttct
    88261 gctcgctggt atattttcgt gcggcacatg ccaggcgaca ggggcagaag tgacgagtga
    88321 atctgtattt aacattctta attccacggg ggcagcaaca gataaaagtt atttatcgtt
    88381 aaatccagat aagtatccta attatcgtct tctgattcac tcagccaaat tacaaaatga
    88441 aataaagagc cactatacca aagacgaaat tcaaggattg ctaacattaa cagaaaacac
    88501 acgaaagtta acgttgacgg agaaaccctg gggaacgttt attcttgctt ctactttcga
    88561 agatgacaaa acagcggcag aaacccacta tgatgctgtc tggctgaggg atagtttatg
    88621 gggctatatg gcattagtat cagaccaggg aaatagtgta gccgcaaaaa aggttctgct
    88681 caccctgtgg gattatatgt ctacgcccga ccagattaag cggatgcagg acatcattag
    88741 caacccaaaa cgactggatg gtattcccgg gcaaatgaat gctgtgcata tttgacagca
    88801 actcccccgt gatggcagat gtgcaggaag aaggcaagcc gcagctgtgg aaccataaac
    88861 aaaatgatgc gttaggtctt tatcttgatc tccttataca agcaatcaat actggtacta
    88921 ttaacgctga agattggcaa aaaggtgacc gactaaaatc ggt
//
LOCUS       DAZFAB010000007        85171 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZFAB010000007 DAZFAB010000000
VERSION     DAZFAB010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 85171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 85171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..85171
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.007"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(171..842)
                     /gene="queE"
                     /locus_tag="ACHJWA_000589"
     CDS             complement(171..842)
                     /gene="queE"
                     /locus_tag="ACHJWA_000589"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFO4307248.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     gene            997..1617
                     /locus_tag="ACHJWA_000590"
     CDS             997..1617
                     /locus_tag="ACHJWA_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HFO4307249.1"
                     /translation="MKRDLNTLRIFIVIIFIFNLSGCGYNDIQTYDEQVNASWSEVLN
                     QYQRRADLIPNLVASIKGYSSHERDVLEAVTLARSQANRASSDLQQTPGDEQKLQAWQ
                     QAQAQVTRTLGQLTIISERYPELKSQELYQNLMVQLEGSENRIAVARGRYIKAIEQYN
                     VTIRKFPAVLTAKVMDYTPKKNYLPDDVAAVSKAPTIDFSQNANAH"
     gene            1631..2305
                     /locus_tag="ACHJWA_000591"
     CDS             1631..2305
                     /locus_tag="ACHJWA_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFO4307250.1"
                     /translation="MRNFIFLMAFFCSSVFATQIPVPESPKYVNDLTGTLTNSEVNTL
                     TNQIKALTQKNYAQLVVLVVETTGDETIEQYATRVFDSWKPGDKDRDDGVLLLVAWQD
                     HTVRIEIGYGLEGIITDAQSGKIIRNSIIPAFKKGDLAGGLQKGISDIESRLTGNNLA
                     TITPTDHPLPFSGWWALLVWAIVLTFISARGYIKTLGVICFAAIVLAFVLPIAGFSGS
                     WGVLAT"
     gene            2539..3687
                     /locus_tag="ACHJWA_000592"
     CDS             2539..3687
                     /locus_tag="ACHJWA_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFO4307251.1"
                     /translation="MRIFLLLLMLCGFQVFAERLPSVTDLTGTLTTEEQTALTQQLQT
                     LEQQNHFQVAVLVTPTTGGKNIKLAASQRYGVYHWKPVGEKRYGEGILILVVWPEGLA
                     SMKIGHGLEEMLPPEQAAQIVRYHMQPEFEKNNLFAGLTGGIESIAQFTHIKANLSPL
                     DALANHLFANPQRSLPCLAWTLLMIVAIVVLWLFASRPRRGIWMISMITPMVWFFSFQ
                     DDVIIRRVSVVCFSLLLATTCKQRLAVVFNMCRVFPMMLTPKNKNAKVKKQKKTPQVR
                     ESGRNSLFVVMLIIIVGWCLVFASNKDIAFISTIIGLIDHFIGPITIAGIVLLIIVAK
                     FTGNLKLGSGEKSNRKTGNRNAHSRSSSRNSFRGGGGSSGGGGSSVRW"
     gene            3684..4598
                     /locus_tag="ACHJWA_000593"
     CDS             3684..4598
                     /locus_tag="ACHJWA_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311665.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFO4307252.1"
                     /translation="MIVMRLIFILFTLWCLPGLAQQIAVPELRQQVTDITGTLSTSEQ
                     QSLTQQLQDITHKTRAQVAVLVVPSTGDDSIEQYATRVFDSWKLGDKQRNDGILLLVA
                     WEDHAVRIEVGYGLEGVVTDLQAAKIIRDILIPAFKSDDLMGGLTLASENIGALLLNG
                     ELPEDRGDYYSINPPIPLSLAVIILLAVLSYFIVFTDPSNLPWITLTGAIYGMVFLYV
                     AEPGPWTNLIVACGMLTPFAIVPLVIFWLIVNKKLRAKYKKLSKDRASRKGSSSSSSG
                     GGSSGGGSGGGFSGGGGSSGGGGASGRW"
     gene            complement(4658..5956)
                     /gene="eno"
                     /locus_tag="ACHJWA_000594"
     CDS             complement(4658..5956)
                     /gene="eno"
                     /locus_tag="ACHJWA_000594"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFO4307253.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(6044..7681)
                     /gene="pyrG"
                     /locus_tag="ACHJWA_000595"
     CDS             complement(6044..7681)
                     /gene="pyrG"
                     /locus_tag="ACHJWA_000595"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFO4307254.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(7909..8700)
                     /gene="mazG"
                     /locus_tag="ACHJWA_000596"
     CDS             complement(7909..8700)
                     /gene="mazG"
                     /locus_tag="ACHJWA_000596"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFO4307255.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(8771..9106)
                     /gene="mazF"
                     /locus_tag="ACHJWA_000597"
     CDS             complement(8771..9106)
                     /gene="mazF"
                     /locus_tag="ACHJWA_000597"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HFO4307256.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(9106..9354)
                     /gene="mazE"
                     /locus_tag="ACHJWA_000598"
     CDS             complement(9106..9354)
                     /gene="mazE"
                     /locus_tag="ACHJWA_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HFO4307257.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(9432..11666)
                     /gene="relA"
                     /locus_tag="ACHJWA_000599"
     CDS             complement(9432..11666)
                     /gene="relA"
                     /locus_tag="ACHJWA_000599"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFO4307258.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(11714..13015)
                     /gene="rlmD"
                     /locus_tag="ACHJWA_000600"
     CDS             complement(11714..13015)
                     /gene="rlmD"
                     /locus_tag="ACHJWA_000600"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFO4307259.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLEVEPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNCLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            13072..15828
                     /gene="barA"
                     /locus_tag="ACHJWA_000601"
     CDS             13072..15828
                     /gene="barA"
                     /locus_tag="ACHJWA_000601"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFO4307260.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGCGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(16061..17401)
                     /gene="gudD"
                     /locus_tag="ACHJWA_000602"
     CDS             complement(16061..17401)
                     /gene="gudD"
                     /locus_tag="ACHJWA_000602"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFO4307261.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLLTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(17422..18762)
                     /gene="gudX"
                     /locus_tag="ACHJWA_000603"
                     /pseudo
     CDS             complement(17422..18762)
                     /gene="gudX"
                     /locus_tag="ACHJWA_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
     gene            complement(18764..20116)
                     /gene="gudP"
                     /locus_tag="ACHJWA_000604"
     CDS             complement(18764..20116)
                     /gene="gudP"
                     /locus_tag="ACHJWA_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFO4307262.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIAISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVVGQ
                     "
     gene            complement(20551..21000)
                     /gene="yqcA"
                     /locus_tag="ACHJWA_000605"
     CDS             complement(20551..21000)
                     /gene="yqcA"
                     /locus_tag="ACHJWA_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFO4307263.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(21018..21800)
                     /gene="truC"
                     /locus_tag="ACHJWA_000606"
     CDS             complement(21018..21800)
                     /gene="truC"
                     /locus_tag="ACHJWA_000606"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFO4307264.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGERSS"
     gene            complement(21800..22129)
                     /gene="yqcC"
                     /locus_tag="ACHJWA_000607"
     CDS             complement(21800..22129)
                     /gene="yqcC"
                     /locus_tag="ACHJWA_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFO4307265.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(22751..23296)
                     /gene="syd"
                     /locus_tag="ACHJWA_000608"
     CDS             complement(22751..23296)
                     /gene="syd"
                     /locus_tag="ACHJWA_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFO4307266.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLAPNLAEFLNQLKPLL"
     gene            23364..24212
                     /gene="queF"
                     /locus_tag="ACHJWA_000609"
     CDS             23364..24212
                     /gene="queF"
                     /locus_tag="ACHJWA_000609"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311681.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFO4307267.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFQGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGEVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            24324..25688
                     /gene="ppnN"
                     /locus_tag="ACHJWA_000610"
     CDS             24324..25688
                     /gene="ppnN"
                     /locus_tag="ACHJWA_000610"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFO4307268.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            26245..27534
                     /gene="sdaC"
                     /locus_tag="ACHJWA_000611"
     CDS             26245..27534
                     /gene="sdaC"
                     /locus_tag="ACHJWA_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFO4307269.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            27592..28959
                     /gene="sdaB"
                     /locus_tag="ACHJWA_000612"
     CDS             27592..28959
                     /gene="sdaB"
                     /locus_tag="ACHJWA_000612"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFO4307270.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMCG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            29071..29826
                     /gene="xni"
                     /locus_tag="ACHJWA_000613"
     CDS             29071..29826
                     /gene="xni"
                     /locus_tag="ACHJWA_000613"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFO4307271.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(29881..31029)
                     /gene="fucO"
                     /locus_tag="ACHJWA_000614"
     CDS             complement(29881..31029)
                     /gene="fucO"
                     /locus_tag="ACHJWA_000614"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFO4307272.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(31057..31704)
                     /gene="fucA"
                     /locus_tag="ACHJWA_000615"
     CDS             complement(31057..31704)
                     /gene="fucA"
                     /locus_tag="ACHJWA_000615"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFO4307273.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            32251..33567
                     /gene="fucP"
                     /locus_tag="ACHJWA_000616"
     CDS             32251..33567
                     /gene="fucP"
                     /locus_tag="ACHJWA_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFO4307274.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGSHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            33600..35375
                     /gene="fucI"
                     /locus_tag="ACHJWA_000617"
     CDS             33600..35375
                     /gene="fucI"
                     /locus_tag="ACHJWA_000617"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFO4307275.1"
                     /translation="MNKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            35484..36902
                     /gene="fucK"
                     /locus_tag="ACHJWA_000618"
     CDS             35484..36902
                     /gene="fucK"
                     /locus_tag="ACHJWA_000618"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFO4307276.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIERLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGAEQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPLKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            36904..37326
                     /gene="fucU"
                     /locus_tag="ACHJWA_000619"
     CDS             36904..37326
                     /gene="fucU"
                     /locus_tag="ACHJWA_000619"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFO4307277.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            37384..38115
                     /gene="fucR"
                     /locus_tag="ACHJWA_000620"
     CDS             37384..38115
                     /gene="fucR"
                     /locus_tag="ACHJWA_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFO4307278.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTG"
     gene            complement(38159..39259)
                     /gene="rlmM"
                     /locus_tag="ACHJWA_000621"
     CDS             complement(38159..39259)
                     /gene="rlmM"
                     /locus_tag="ACHJWA_000621"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFO4307279.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(39252..39647)
                     /gene="ygdD"
                     /locus_tag="ACHJWA_000622"
     CDS             complement(39252..39647)
                     /gene="ygdD"
                     /locus_tag="ACHJWA_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFO4307280.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(39666..40583)
                     /gene="gcvA"
                     /locus_tag="ACHJWA_000623"
     CDS             complement(39666..40583)
                     /gene="gcvA"
                     /locus_tag="ACHJWA_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFO4307281.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(40934..41161)
                     /gene="ygdI"
                     /locus_tag="ACHJWA_000624"
     CDS             complement(40934..41161)
                     /gene="ygdI"
                     /locus_tag="ACHJWA_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFO4307282.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            41353..42558
                     /gene="csdA"
                     /locus_tag="ACHJWA_000625"
     CDS             41353..42558
                     /gene="csdA"
                     /locus_tag="ACHJWA_000625"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFO4307283.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGIVVIVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAENWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            42558..43001
                     /gene="csdE"
                     /locus_tag="ACHJWA_000626"
     CDS             42558..43001
                     /gene="csdE"
                     /locus_tag="ACHJWA_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFO4307284.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(43052..43858)
                     /gene="tcdA"
                     /locus_tag="ACHJWA_000627"
     CDS             complement(43052..43858)
                     /gene="tcdA"
                     /locus_tag="ACHJWA_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFO4307285.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINSECCV
                     TVVDDFVTPDNVAQHMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(44097..45194)
                     /gene="mltA"
                     /locus_tag="ACHJWA_000628"
     CDS             complement(44097..45194)
                     /gene="mltA"
                     /locus_tag="ACHJWA_000628"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFO4307286.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            45403..45479
                     /locus_tag="ACHJWA_000629"
     tRNA            45403..45479
                     /locus_tag="ACHJWA_000629"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45437..45439,aa:Met,seq:cat)
     gene            complement(45553..46806)
                     /gene="amiC"
                     /locus_tag="ACHJWA_000630"
     CDS             complement(45553..46806)
                     /gene="amiC"
                     /locus_tag="ACHJWA_000630"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFO4307287.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            47038..48369
                     /gene="argA"
                     /locus_tag="ACHJWA_000631"
     CDS             47038..48369
                     /gene="argA"
                     /locus_tag="ACHJWA_000631"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFO4307288.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(48431..50257)
                     /gene="recD"
                     /locus_tag="ACHJWA_000632"
     CDS             complement(48431..50257)
                     /gene="recD"
                     /locus_tag="ACHJWA_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFO4307289.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEESHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPTGDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRNPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(50257..53799)
                     /gene="recB"
                     /locus_tag="ACHJWA_000633"
     CDS             complement(50257..53799)
                     /gene="recB"
                     /locus_tag="ACHJWA_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFO4307290.1"
                     /translation="MSDVAETLDPLRLPFQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAKWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     RNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVSAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMMRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLNFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQARGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYERHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIDLMDEMFAGMTLEEA"
     gene            complement(53792..56680)
                     /gene="ptrA"
                     /locus_tag="ACHJWA_000634"
     CDS             complement(53792..56680)
                     /gene="ptrA"
                     /locus_tag="ACHJWA_000634"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFO4307291.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEALKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(56856..60224)
                     /gene="recC"
                     /locus_tag="ACHJWA_000635"
     CDS             complement(56856..60224)
                     /gene="recC"
                     /locus_tag="ACHJWA_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFO4307292.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNALFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVSGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(60237..60560)
                     /gene="ppdC"
                     /locus_tag="ACHJWA_000636"
     CDS             complement(60237..60560)
                     /gene="ppdC"
                     /locus_tag="ACHJWA_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFO4307293.1"
                     /translation="MSASLKNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLTSR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGRAGEMTRLH
                     CPNRQ"
     gene            complement(60545..60952)
                     /gene="ygdB"
                     /locus_tag="ACHJWA_000637"
     CDS             complement(60545..60952)
                     /gene="ygdB"
                     /locus_tag="ACHJWA_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFO4307294.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCLLYAATGARVCLRLLEDNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(60949..61512)
                     /gene="ppdB"
                     /locus_tag="ACHJWA_000638"
     CDS             complement(60949..61512)
                     /gene="ppdB"
                     /locus_tag="ACHJWA_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO4307295.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGICTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEQVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(61503..61973)
                     /gene="ppdA"
                     /locus_tag="ACHJWA_000639"
     CDS             complement(61503..61973)
                     /gene="ppdA"
                     /locus_tag="ACHJWA_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO4307296.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISFIREGTLWCLVSSVAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIGFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(62157..62951)
                     /gene="thyA"
                     /locus_tag="ACHJWA_000640"
     CDS             complement(62157..62951)
                     /gene="thyA"
                     /locus_tag="ACHJWA_000640"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFO4307297.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(62958..63833)
                     /gene="lgt"
                     /locus_tag="ACHJWA_000641"
     CDS             complement(62958..63833)
                     /gene="lgt"
                     /locus_tag="ACHJWA_000641"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFO4307298.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(63984..66230)
                     /gene="ptsP"
                     /locus_tag="ACHJWA_000642"
     CDS             complement(63984..66230)
                     /gene="ptsP"
                     /locus_tag="ACHJWA_000642"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO4307299.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(66243..66773)
                     /gene="rppH"
                     /locus_tag="ACHJWA_000643"
     CDS             complement(66243..66773)
                     /gene="rppH"
                     /locus_tag="ACHJWA_000643"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFO4307300.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(67126..67272)
                     /gene="ygdT"
                     /locus_tag="ACHJWA_000644"
     CDS             complement(67126..67272)
                     /gene="ygdT"
                     /locus_tag="ACHJWA_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFO4307301.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            67458..68147
                     /gene="mutH"
                     /locus_tag="ACHJWA_000645"
     CDS             67458..68147
                     /gene="mutH"
                     /locus_tag="ACHJWA_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFO4307302.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            68216..68929
                     /gene="ygdQ"
                     /locus_tag="ACHJWA_000646"
     CDS             68216..68929
                     /gene="ygdQ"
                     /locus_tag="ACHJWA_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO4307303.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            69067..69285
                     /gene="ygdR"
                     /locus_tag="ACHJWA_000647"
     CDS             69067..69285
                     /gene="ygdR"
                     /locus_tag="ACHJWA_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFO4307304.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            69393..70433
                     /gene="tas"
                     /locus_tag="ACHJWA_000648"
     CDS             69393..70433
                     /gene="tas"
                     /locus_tag="ACHJWA_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFO4307305.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLAPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(70465..71658)
                     /gene="lplT"
                     /locus_tag="ACHJWA_000649"
     CDS             complement(70465..71658)
                     /gene="lplT"
                     /locus_tag="ACHJWA_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFO4307306.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHEQLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(71651..73810)
                     /gene="aas"
                     /locus_tag="ACHJWA_000650"
     CDS             complement(71651..73810)
                     /gene="aas"
                     /locus_tag="ACHJWA_000650"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFO4307307.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDPQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEVEQHDE"
     gene            74396..75427
                     /gene="galR"
                     /locus_tag="ACHJWA_000651"
     CDS             74396..75427
                     /gene="galR"
                     /locus_tag="ACHJWA_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFO4307308.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLLKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(75434..76696)
                     /gene="lysA"
                     /locus_tag="ACHJWA_000652"
     CDS             complement(75434..76696)
                     /gene="lysA"
                     /locus_tag="ACHJWA_000652"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFO4307309.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMFDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVEPRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            76818..77753
                     /gene="lysR"
                     /locus_tag="ACHJWA_000653"
     CDS             76818..77753
                     /gene="lysR"
                     /locus_tag="ACHJWA_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFO4307310.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLVRYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDELCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(77740..78432)
                     /gene="ygeA"
                     /locus_tag="ACHJWA_000654"
     CDS             complement(77740..78432)
                     /gene="ygeA"
                     /locus_tag="ACHJWA_000654"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFO4307311.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(78561..79979)
                     /gene="araE"
                     /locus_tag="ACHJWA_000655"
     CDS             complement(78561..79979)
                     /gene="araE"
                     /locus_tag="ACHJWA_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFO4307312.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(80294..81055)
                     /gene="kduD"
                     /locus_tag="ACHJWA_000656"
     CDS             complement(80294..81055)
                     /gene="kduD"
                     /locus_tag="ACHJWA_000656"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFO4307313.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(81085..81921)
                     /gene="kduI"
                     /locus_tag="ACHJWA_000657"
     CDS             complement(81085..81921)
                     /gene="kduI"
                     /locus_tag="ACHJWA_000657"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFO4307314.1"
                     /translation="MDVRQSIHSAHAKTLETQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIIGGIMPVTKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTGTPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(82208..83389)
                     /gene="yqeF"
                     /locus_tag="ACHJWA_000658"
     CDS             complement(82208..83389)
                     /gene="yqeF"
                     /locus_tag="ACHJWA_000658"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFO4307315.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMTQSNGQTLVVDTDEQ
                     PHTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            83644..84873
                     /gene="yqeG"
                     /locus_tag="ACHJWA_000659"
     CDS             83644..84873
                     /gene="yqeG"
                     /locus_tag="ACHJWA_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO4307316.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT        21024 a        22716 c        20655 g        20776 t
ORIGIN      
        1 accgcaataa ttttcactcc agcgaaaaat taacgccaca gaatttgtag agaatgataa
       61 tgattagtcc gggataggca agatggatga gttttcagag acgacaaaga atataaatga
      121 gtagaagtct ttacaggttc cacccattta taacgcttat aaatgtttaa tcaggcaata
      181 tttaaatatt tatgggtttg catcgacaaa cgccaattac gcgcaatgca ggtttcaatg
      241 cacaaacgtg tggcatcatc cttttggcta atcggctgca gtgcaatgac tcgcggttta
      301 tcatcggtca gcgtcgccaa aagttcatcc agtgcttcaa tatcacgtac gcgtcccact
      361 ggatgcttga tttcgttggc tcgctccagt gcctgtgaca acacttcata gccgccgcgc
      421 atgttcagct ttggcgatac ggtaacccag gtattcggtg tacagcgtac ttcatgagta
      481 ccactggttt cgatctggca gctaaaaccg ttcttttcga gcaggtcagt cagtggcagc
      541 aaatcatgaa tgcaaggctc accacccgta atcaccacat gccgcgcggt atatccctgg
      601 cgaccaatga cagccagcaa gtcttcactg ctcgccgccc cccacttatc actctctttg
      661 gtcttcgcca gaatgctgaa aagggagact tcccgatcct caagcttttc ccaggtgtgt
      721 ttggtgtcgc accaggcaca gccaaccggg catccctgta aacgaataaa aatggcgggg
      781 acgccggtaa agtaaccctc accttgcagg gtctggaaca tctcgttaat cgggtactgc
      841 atagcattct ctgtgaagtg gataattgtt aattattgca gatcctgcca caacaatcat
      901 gtcttattaa cattctgtta caggcaggtt tatggcaaaa agtggattag atcataaaga
      961 ataaaatgaa gcagagtatt atgttttaag gaattaatta aaagggattt aaacacgctc
     1021 agaatattca tcgtaattat atttattttc aatttatcag gctgtggata taacgatatt
     1081 caaacctatg atgagcaggt taacgcctcc tggtctgagg tattgaatca atatcaacgt
     1141 cgcgccgatc ttattcctaa tcttgtcgcc agcattaaag gttattccag ccatgaacga
     1201 gatgtactgg aagccgtaac attggctcgt agccaggcta atcgcgccag tagcgatctt
     1261 cagcaaactc cgggagatga acaaaaatta caagcctggc aacaggctca agcgcaggtg
     1321 actcgcactc tcggtcagtt aacgataatt agtgagcgtt accctgagtt aaaatctcag
     1381 gaactctatc aaaatttaat ggtacagctt gaaggaagtg aaaatcggat tgccgttgcg
     1441 cgaggaagat atattaaagc cattgaacaa tataatgtca ctatccgcaa attccccgct
     1501 gtattaaccg caaaggtcat ggattacaca ccaaagaaaa attatttgcc ggatgatgtc
     1561 gccgcagtaa gtaaagcccc aacaatagat tttagccaga atgctaatgc tcattaagcg
     1621 gggaagttct atgcgaaatt ttatcttcct tatggctttc ttctgttcat ctgtgtttgc
     1681 cacacaaatt ccagtacctg aatcacccaa gtatgtgaat gacttaaccg gcacattaac
     1741 aaacagcgaa gtaaacaccc tgaccaacca gattaaagcc ctgacgcaaa aaaactacgc
     1801 acagttggtc gtgttagtgg ttgaaacaac aggcgatgaa accatcgagc agtacgcgac
     1861 gcgggtattc gatagctgga aaccgggtga taaagaccgc gatgatggcg ttctactgtt
     1921 agtcgcctgg caggatcata ccgtgcgtat tgaaatcggt tacggactgg aaggtattat
     1981 tacggatgcg cagtccggga aaattattcg caacagtatt attcctgcat ttaaaaaagg
     2041 cgacctggct ggtggtttgc aaaagggaat cagcgatatt gaaagtcgtc tgacggggaa
     2101 taatttagca acgataacac cgaccgatca ccctttgccc tttagcggtt ggtgggcatt
     2161 gttagtctgg gcgatagtac tgacgtttat ttctgccaga ggatatatca aaacgctggg
     2221 agtaatttgc ttcgcagcta ttgtactggc ctttgtttta cctatagcag gcttcagcgg
     2281 aagctgggga gtactcgcaa catgactctg cttcgccaca ccgttcctgg ctgtcgccat
     2341 cgcttttacc cccttcggca aaaaagttcg ggatttgatg cgtgatgcca accagccatc
     2401 acgccataca agaagcaatt cttcctccca ctattcatca tcgtcatcct cttcattcga
     2461 caatgacaat tttagcggtg gtggcggttc atctggtggc ggtggcgctt ccgggcgctg
     2521 gtaacaaaag gaattgatat gcgtattttt ctcttacttc tgatgctatg cggttttcag
     2581 gtattcgctg aacgattgcc ttcagtgact gacttaaccg ggacattaac gacagaagaa
     2641 caaactgctt taactcaaca attacagact ctcgagcagc aaaaccactt ccaggtggcc
     2701 gtgttggtta cgccgaccac agggggcaaa aacattaaac ttgcagcctc gcagagatac
     2761 ggggtatatc actggaaacc cgttggagag aagcgatatg gcgaaggtat cttaattctc
     2821 gtcgtatggc cggaagggct ggcgtcaatg aagattggac atggactgga agagatgttg
     2881 ccgccggaac aagctgcgca aattgttcgt taccatatgc agccagaatt cgaaaaaaac
     2941 aatctctttg ctggattaac gggcggaatt gaaagcatcg cacagtttac ccatatcaaa
     3001 gcgaacctta gccctttgga tgctttagcc aatcatctgt ttgctaatcc gcaacgttcg
     3061 ctgccatgtc ttgcctggac actgctgatg atcgttgcca tcgttgtcct ctggctattt
     3121 gccagccgcc cacggcgtgg catatggatg atatcgatga ttacccctat ggtctggttt
     3181 ttctcctttc aggacgatgt catcattcgg cgggtatcgg tcgtttgttt tagtttattg
     3241 ttagcaacaa cctgcaagca gcgtctcgcg gtagttttta atatgtgccg cgtatttcct
     3301 atgatgctga ccccaaaaaa taaaaacgct aaagtgaaaa aacaaaagaa gactccgcag
     3361 gttcgtgaat caggacgaaa ttcattgttt gtcgtgatgc tgataattat tgtgggatgg
     3421 tgcctggtgt tcgccagtaa taaagatatt gcattcatct caacaattat tgggttaatt
     3481 gatcatttta tcggcccaat aaccattgct ggcatcgttc tgctgatcat tgtggccaaa
     3541 tttactggca atcttaagct ggggtcagga gaaaaaagca acaggaaaac ggggaatcgt
     3601 aatgctcatt ctcgctcttc gtcgagaaat tctttccgcg gtggtggcgg ttcttcagga
     3661 ggcggcggtt catccgtccg ttggtgatcg taatgcgctt aatctttatt ctattcactt
     3721 tgtggtgcct tcctgggctt gcccagcaaa ttgctgtgcc ggaactgcgc caacaggtga
     3781 ccgatattac tggtacgtta agcacttctg aacaacaatc actgacacag caactgcagg
     3841 atattacaca taaaacacgg gcgcaggtcg ctgtattagt agtgccgtcc accggagacg
     3901 attccattga acaatatgct acaagggttt ttgatagctg gaaactggga gataagcaga
     3961 gaaacgatgg tattttactt ctggttgcct gggaggatca tgctgttcgt attgaggttg
     4021 gctacgggct ggaaggggtg gttaccgacc tgcaagcggc gaagattatc agggatatat
     4081 tgatccctgc cttcaaaagt gatgacctga tgggtggatt aacactggca agcgaaaata
     4141 tcggcgcgct tctgttgaat ggtgaattac cggaagacag gggggattat tacagtatca
     4201 atccccctat tccattatcg cttgctgtaa ttatattgct ggcggtactt tcatatttta
     4261 ttgtttttac cgatccgtcg aatttaccct ggatcactct cactggcgcc atttacggga
     4321 tggtattcct ctatgttgcc gagccaggac catggaccaa tttaatcgtt gcctgcggta
     4381 tgttaacgcc ttttgcgatc gtaccactgg ttattttttg gcttatcgtc aataaaaagc
     4441 tacgcgccaa atacaaaaaa ctcagtaaag atagagcttc aagaaaaggt tcttcttcat
     4501 cctcttccgg tggcggctca agtggcggag gctcaggcgg cggatttagc ggcgggggcg
     4561 gttcttctgg cggtggcggc gcgtccggtc gctggtaact caaccataaa aaacgccagc
     4621 ccgaaggctg gcatttttaa atcagataaa gtcagactta tgcctggcct ttgatctctt
     4681 tacgaccgtt gtacggtgct ttttcgccca gagcttcttc gatacgaatc agctggttgt
     4741 atttagcaac gcggtcagaa cggctcatag aaccagtttt gatctggcct gcagcagtac
     4801 caacagccag gtcagcgatg gtagcgtctt cagtttcgcc agaacggtga gagataactg
     4861 cagtgtagcc agcgtctttc gccatcttga ttgcagccag agtttcggtc agagaaccga
     4921 tctggttgaa tttgatcagg atggagttag cgataccttt ttcgatacct tctttcagga
     4981 tcttggtgtt ggttacgaac aggtcgtcac caaccagctg gattttgtcg cccagaactt
     5041 tggtctggta tgcgaaacca tcccagtcag attcgtccag accgtcttcg atggaaacga
     5101 tcgggtactg tttggtcagt tcttccagga agtgagtgaa ttcttcagag gtgaatgctt
     5161 tgttgccttc gccagccaga acgtatttac cgtctttgta gaattcagaa gctgcgcagt
     5221 ccatcgccag agtgatgtct ttgcccagtt cataaccagc agctttaaca gcttcagcga
     5281 taacagccag agcttcagcg ttggaaccca ggttcggcgc atagccacct tcgtcaccaa
     5341 cagcagtgtt catgcctttc gctttcagaa cttttgccag gtgatggaaa acttcagaac
     5401 ccatgcggat ggcttctttc acagttttcg cgccaaccgg ctgaatcatg aattcctgga
     5461 tatcaacgtt gttgtcagcg tgctcaccac cgttgatgat gttcatcatc ggaaccggca
     5521 tggagtattt gcccggagtg ccgttcagtt cagcgatgtg ctcgtacagc ggcatacctt
     5581 tagcagctgc agcagctttg gcgttagcca gagatacagc caggattgcg ttcgcgccga
     5641 atttggattt gttttcggtg ccgtccaggt cgatcatgat cttgtcaatg ccagcctgat
     5701 ctttagcatc tttgccaatc agcgcctgag cgatcgggcc gtttaccgca gcaacagctt
     5761 tggttacgcc tttacccagg aaacgggatt tgtcgccatc gcgcagttcc agagcttcac
     5821 gggaaccagt agaagcacct gacggagcag ctgccatacc gacgaaacca ccctccagat
     5881 gtacttcggc ttcaacagtc gggttaccac gggagtcgat gatttcacga ccgatgattt
     5941 ttacgatttt ggacattagg ttttcctcaa gtcactagtt aaactgaaac tccagacaaa
     6001 caacgcgtac ccagggtacg cgttgccgtt ctaacttttt tacttacttc gcctgacgtt
     6061 tctggaactc gctggcggct ttcacaaagc ctgcaaacag cgggtgacca tcacgtggag
     6121 tagaagtaaa ctccggatgg aactggcaag ccacgaacca cgggtgattc ggaacttcga
     6181 tgatctcgac caactgatca tccccggaac ggcccgcaac gcgcagacct gcatcttcaa
     6241 tctgtttcaa cagcatgttg ttgacttcgt aacggtgacg atgacgctca acaattgtcg
     6301 gcgcattgta cagctggcga accaggctat cgtcaaccaa ctggcactgc tgtgcgccga
     6361 gacgcatggt accgccgaga tcgctcttct cgctacgaac ttcaacgttg ccgttttcat
     6421 cgcgccactc ggtaatcagc gccacaaccg ggtacttaca gtctggcaca aattccgtag
     6481 agttggcgtt ctccatgttg gcaacatggc gagcgtaatc aattaacgcc acctgcatac
     6541 ccaggcaaat gcccagataa ggaatattgt tctcacgcgc aaaacgcgcg gtcgtaatca
     6601 tgccttctac gccacgatag ccgaaaccgc caggtacgag gattgcatcc agacctttaa
     6661 ggatttcaac gccgcgcgtt tcaacatctt gtgaatcgat cagtttgatg ttgacgctga
     6721 cacgattctt cagcccaccg tgtttcagtg cttcaatcac ggatttgtaa gcgtccggca
     6781 gttcaatgta cttgccgacc ataccaatgg tgacttcact taccggattc gcttcttcga
     6841 agataacctg ttcccattcg gacagatttg cttccgggca gtttaagctg aatcgtttac
     6901 aaatataatc gtccagcccc tgagatttca acaggcccgg aattttatag atggaatcga
     6961 cgtctttcag agaaataacc gctttttccg gaacattaca gaacaatgca atcttcgcac
     7021 gttcgttcgc cggaacggcg cgatctgaac gacaaatcag gatgtcaggc tggataccga
     7081 tggagagcag ctcttttaca gagtgctgag tcggtttggt tttgacttca ccagacgctg
     7141 ccatgtacgg caccagcgtc aggtgcataa acagagtgtg ctcacggcca atttcaacag
     7201 ccatctggcg aatcgcttcg aggaacggca gggattcgat atcacctact gtaccgccga
     7261 tttcgaccag tactacgtca tgaccttcgc caccttccag cacgcgctct ttgattgcgt
     7321 tagtgatgtg cggaataacc tgtacggttg cgccgaggta gtcaccgcgg cgttctttac
     7381 gcagaacgtc agagtagata cgacccgtgg tgaagttgtt gcggcggctc attttggtac
     7441 ggatgaaacg ctcgtagtgc cccaggtcca ggtcggtttc agcgccgtct tcagtaacga
     7501 acacttcccc gtgttggatt gggctcatag tacctggatc gacgttgatg tacggatcca
     7561 gtttcatgat ggtcacattg aggccacggg cttcaagaat ggctgcgagg gaggctgcgg
     7621 caatgccttt acccagagag gatacgaccc cgccggtcac aaaaatatag ttcgttgtca
     7681 tgctgaacct gagaagttag gttgaaaaga cgatggaata accaggacgg gaaagcagta
     7741 tacccgaaca tgacctgtgc cacaaacttt cattatccct cctcttcgcc agcgcactat
     7801 tgaaatcagg agtgagaaaa tagccccttt ggggtaaatg tttttgacgc aaatcaagcg
     7861 cttgtcattt aaaaaatgac acaaatggcg cttgaccgcg taattccctt agagatcaat
     7921 ttcctgccgt tttacctgtt gccagacttc ttccattgtt tcgaggtcaa cacctgtcat
     7981 ttccagtcca cgcgcggcaa caatacgctc cacttcgcga aaacgacgct cgaatttttc
     8041 gttcgctttt tgcaatgcga tttccgcttt tgtccctaaa tgacgagcca gattgaccgt
     8101 ggcaaacagc aggtccccca tttcctcctc cagtttagcc tggtcgacaa cagcctgccg
     8161 cgcttcgtac atcacctcgt cgatctcttc gtagacttta tcgactaccg gaccaagcgt
     8221 cgtccaatcg aagccaacgt tggcgcaacg tttctggatt ttttgcgcac gcattaaagc
     8281 cggtaaacta cgaggaatat cgtccagcgc cgaatgctgc gctttctgcg cgcgctcttc
     8341 ggttttgatt tgctcccaac gggcaagcac ttcactactg ttttcggcag aactgtcagc
     8401 aaaaacatgc ggatggcgac gctctaattt atcgctaatc gcagcgcaaa tatcattaaa
     8461 gtcaaagcgc ccttcttcct gagccatttg ggcgtaaaac accacctgga acagcagatc
     8521 gccgagttca ccgcgcagat cgtcaaaatc ttcacgggcg atggcgtcca gcacttcgta
     8581 ggtttcttca agggtgtaag gcgcaatggt ggcaaatgtc tgctctttat cccacgggca
     8641 gccgttttcc gggtcgcgca ggcgctgcat aatagtgagc aaacggtcga tttgattcat
     8701 tagaatgtcc tgaaaattgc gggtccgtca ggtggaaacc tgtgaccaga atagaagtga
     8761 gttagtaaca ctacccaatc agtacgttaa ttttggcttt aatgagttgt aattcctctg
     8821 gggcaaccgt tcctttcttc gttgctcctc ttgcccgcca ggcgatactt tttacctgat
     8881 cagctaacgc tacgccatca cgttcctgac cggataaaac aacttcgaac ggatatcctt
     8941 ttgattgcgt tgtacaagga acacacagac acatacctgt tttgttgttg tacatgaacg
     9001 gactcaggac aacagccgga cgatgtccgg cttgctcgct accttttgtc gggtcaaaat
     9061 caacccaaat cagatcgccc atatcgggta cgtatcggct taccattacc agacttcctt
     9121 atctttcggc tctccccagt cgatattctc gtggaggttt tccggcgtga tgtcgttgac
     9181 cagttcagca agcgtaaata cgggctcttt acgcactggc tcaataatta atttgccatc
     9241 caccaggtca atcttcactt catcatcaat attcagattg agcgcctgca ttaacgtagc
     9301 cgggatccgc accgccggtg aatttcccca acgctttacg ctactgtgga tcataaccct
     9361 ttcctcaaac cactatcata tgtagataca gtatatatca atctacattg tagatacgag
     9421 caaatttcgg cctaactccc gtgcaaccga cgcgcgtcga taacatccgg cacctggttg
     9481 agtttaccca gcacgcgccc cagcacttgc aggttgtaaa tctcaatggt catgtcgatg
     9541 gtcgccagtt gctgtttggt gtcgctacgg ctggcaacgc caagcacgtt caccttctcg
     9601 ttggcgagaa tggtcgtgat atcacgtaac aacccactac gatcattagc caccacgcgg
     9661 accaccagcg aatatccggc ggagtagctc tcaccccata ccgcgtcaac aatgcgttct
     9721 ggcgcatggg agcgcagttc cgccagttgt tcgcaatcgg cgcggtgtac tgaaataccg
     9781 cgtccctggg taatgaagcc gacaatctca tctccaggaa tcggctggca gcagcgcgcg
     9841 atgtggtgca tcaggttacc aacaccttca accactacac gaccgttatc tttactgcgg
     9901 ttttgcggcg tgtagctttt ttgctgaagc tgtttcagcg cggcggcgtc ctgctcttcg
     9961 gcactcggct tattaaattg cgattgcagg aagttcacca tctgattgag acggatatcc
    10021 ccgccaccaa tcgccgccag caactcgtcg acatcattga agttgtaacg cggcagcaga
    10081 tgtttttctg cttctttcag gctgatcccc agatgttcca gctcgtcgtc aaggatttgc
    10141 cgcccagcca gaatgttttt gtcacggtcc tgtttacgga accaggcgtg aattttcgaa
    10201 cgcccacggc tggttgtgac gtaaccgagg tttgggttta accagtcacg gctggggttc
    10261 ggctgtttct gggtgataat ttcaatctgg tcgcccattt gcagctggta ggtgaacggc
    10321 acaatgcgcc cgccaatttt tgccccgata cagcggtgtc cgacatcact gtggatgtgg
    10381 taagcgaagt ccagcggcgt tgatcccgca ggcaaatcaa cgacatcacc tttaggcgta
    10441 aagacgtaca cccggtcgtc aaagacctgg ctgcgtactt cgtcgagcat ttcgccggaa
    10501 tcagccatct cttcctgcca cgcaatcagt ttacgcagcc aggcaatccg gtcttcatgt
    10561 cccgaacgtg cgccgccagc agccgcgccc tctttatatt tccagtgcgc agcaacaccc
    10621 aactctgcat cttcatgcat ctgtttggtg cggatttgga tctcaacggt ttttccaccc
    10681 ggccccagaa ccacggtatg aatagactga taaccgtttg gtttcgggtt agcgacgtaa
    10741 tcgtcaaact catccggcag gtggcgatag tgagtgtgca ctatccccag tgcggcataa
    10801 caatcctgta aacgctcggc gacaatacgt accgcacgca catcaaacag ctcatcgaag
    10861 gcgaggttct ttttctgcat tttgcgccag atgctgtaga tgtgtttcgg acgaccatac
    10921 acttcagctt taacgccttc agctttcatc tcagcgcgca gatgaccgac aaactcttcg
    10981 atatagtgtt cgcggtcgag acggcgttca tgcaacagtt ttgcgatgcg tttgtattcg
    11041 gtcgggtgga gataacggaa gcagtaatct tccagctccc atttcagttg cccaatccca
    11101 agacggtttg ccaacggcgc gtagatattg gtgcactctt ttgccgccag tacgcgttca
    11161 tcttccggcg catcttttac ttcacgcaga tgagcaatac gctccgccag tttgatgacc
    11221 acgcagcgaa aatcatcgac catcgccaat aacatccggc gaacgttatc gacctgttcg
    11281 gaggaaacag aatcagtgtg cgtcgctttc agctggcgga tcgccgccat atcacgcacg
    11341 ccgtgaataa ggttaacgac cgacttaccg acgctctcac gcagcacatc ttcgctgact
    11401 acgttggcat cagccagagg gaacagcagc gccgcccgca gcgtgtcaat gtccatactt
    11461 aatgtcgaga ggatctccac catctcaaca ccacgccaca ataacagact ggcatccgga
    11521 tgcccctgcg tctgttgcag acaatacgcc caggtttcgg ctaagcactc acacgacttc
    11581 tggctggtaa tacccagact tgcgatccat ttttccggat caaattcacc agccttattg
    11641 atatgtgcac ttcttaccgc aaccatcgtc ctctccttta gggaccagac ctgccgaaat
    11701 cggcaaatcg caactattta acgcgcgaga aaagtaccat cgattccaga tgtcccgtgt
    11761 gtgggaacat atccagcatc gccagtcgcg caatggtata tcctgctttt aataacgctt
    11821 cgctatcccg agccagcgtt gcagggttac aggatacata aactatacga ataggttcca
    11881 gttttataat ttgctgcata acacctgcgg cacctgctcg cgccgggtcc agcaacactt
    11941 tatcgaagcc gtttttcgcc cacggctgct ttgtgacatc ttcttcaaga ttttcgtgat
    12001 aaaacgtcac attctgtaag cagttaagac gcgcattctg ctggcctttt tccaccagcg
    12061 ccggaacacc ttctacaccc accacactgg cagcttgtgt cgccaatggt agtgtaaagt
    12121 tgcccatacc gcagaacaga tccagtacgc gatcttcagg ttccacctcc agccattcca
    12181 acgcacgcgc taccattttt tggttcacac ccgcattaac ctgaataaaa tcgcgcgggc
    12241 taaaagttaa gcgcaacccg tttgagtcat accagggcat ctcaccagag acggtttcga
    12301 gtatctcact atcgggggcg agatacagat ccaggccttc agaatgcgaa aagcgttcca
    12361 gtttttcgcg atctgccgaa cttagcggtg cggtatggcg caaaatcatc agcgtgccgc
    12421 tggttgcctg taccagttca acatgaccaa gatggcgcat agcttgtaag ctgcccaggc
    12481 atgccctgac tttgggcagc aatgcttcaa gttggggcac taaaatggga cattgtttaa
    12541 cgtcgacaat gtcactggag cccgctttgc gaaaccccat ctgaagttgc tgtgttttcg
    12601 gtaagtagtt cagacttaaa cgcgcgcgac ggcgatagcc ccagggaaca tcggcgatca
    12661 cttcagagac ttcgtgtttc attaatcggg cgagtgccgc acttttgctt cgctgctgta
    12721 aatccacgct ggcgtgttgt tgctgacagc cgccgcatac gccaaaatga ggacagcgtg
    12781 gcgtttcgcg ttccgggcta tcgcttaacc ggcgtacgac tttagcgcgg gcatactgtt
    12841 ttttatcctc agtaacagta acttccgcgt tttcctgcgg caataatccg gggataaata
    12901 gcgttttgcc gttatgtcgc gccacgccct gaccaaaaga gtcgaggtcg ttgactgaaa
    12961 cggttatgat ctgacgcgtc gtcgtgcgtc gttttgcaga gtagaattgc gccattggcg
    13021 agactttctc aatttaacag tgtgacctta attgtcccat aacggaactc catgaccaac
    13081 tacagcctgc gcgcacgcat gatgattctg atcctggcac cgaccgtcct tattggttta
    13141 ttgctgagta tctttttcgt cgtgcatcgc tataacgact tgcagcgtca actggaagat
    13201 gccggtgcca gcattattga gccgcttgca gtttctactg aatatggcat gagcctgcaa
    13261 aatcgcgaat ctatcggtca gttaataagc gtactgcatc gtcgccattc cgatattgtt
    13321 cgcgcgattt cggtttatga tgaaaataac cgactctttg tcacctccaa ttttcatctt
    13381 gatccctcat caatgcagct cggcagcaac gtgccgtttc ctcgccagct cactgtcact
    13441 cgtgacggcg atattatgat cctccgcacg ccgattattt ctgagagtta ctcccccgac
    13501 gaatcgccca gtagcgatgc caaaaatagt caaaatatgt tgggatatat tgcgcttgag
    13561 ctggatctta aatcggttcg cttgcagcaa tataaagaga tctttatttc cagcgtgatg
    13621 atgctgtttt gtatcggtat tgcgcttatt tttggctggc gcttaatgcg cgatgtaacc
    13681 ggtccgattc gcaacatggt gaatactgtc gaccgcatcc gtcgcgggca actcgacagc
    13741 cgagtggaag gatttatgct cggcgagctg gatatgctga aaaacggtat caactcgatg
    13801 gcaatgtcgc tggctgctta tcacgaagag atgcagcaca atatcgacca ggcgacgtcc
    13861 gatctgcgtg aaacgctgga gcagatggaa attcagaacg ttgagttgga tctggcgaaa
    13921 aagcgcgccc aggaagcggc gcgtattaaa tccgagtttc tggcaaatat gtcacacgag
    13981 ctgcgtacac cactgaatgg tgttattggt tttacccgcc tgacgctgaa aacagaatta
    14041 acaccaacgc agcgcgatca cctgaatacg attgaacgtt cggcaaataa tttgctggca
    14101 attattaatg atgttctcga cttctcgaaa ctggaagcag gtaagctgat tctggaaagt
    14161 attccattcc cactacgcag cacgctggat gaagtcgtta ctctgctggc acattcttct
    14221 cacgataaag ggttggaact gacgctcaat attaaaagcg acgtgcctga taacgtgatc
    14281 ggcgacccac tgcgattaca gcaaatcatc actaacctgg tggggaatgc aattaaattc
    14341 accgagaatg gcaacattga tattctggta gaaaaacgtg cgctgagtaa taccaaagtg
    14401 cagattgaag tgcagattcg ggataccggc attggtattc ctgaacgcga tcaatcgcgc
    14461 ttattccagg ccttccgaca ggctgatgcc agtatttccc gccgtcatgg tggcaccggg
    14521 ctggggctgg tgattacaca aaaactggtt aatgaaatgg gcggcgatat ttcgttccat
    14581 agccagccga atcgcggttc aactttctgg ttccacatta atctcgatct gaacccgaac
    14641 attattattg aagggccatc cacccagtgc ctcgccggta aacgcctggc ctatgtcgaa
    14701 ccaaactccg cagcagcgca atgcacgctg gatattttaa gtgaaacgcc gctggaagtg
    14761 gtttatagcc caacgttctc cgcgctgcct cccgcgcatt acgacatgat gttgttaggc
    14821 atcgcggtga ccttccgcga gccgctaaca atgcaacatg agcgattagc gaaagcggta
    14881 tcgatgaccg atttcctgat gctggcactt ccttgccatg cacaagtcaa tgctgaaaaa
    14941 ctcaagcaag atggtatcgg cgcgtgtctg ctgaaaccat taacacctac gcgcctgttg
    15001 cctgccctga cggaattttg tcatcacaaa caaaacacgc ttttgcctgt aaccgatgaa
    15061 agtaagctgg caatgacagt catggcggtt gatgacaacc ccgctaacct gaaacttatc
    15121 ggcgcattgc tggaagatat ggtgcaacat gtggaacttt gcgatagcgg gcatcaggcg
    15181 gttgaacggg cgaaacagat gccgttcgat ttgatcttaa tggatattca aatgcctgac
    15241 atggatggca ttcgggcctg cgagctcatc caccagctcc cccatcagca acaaacgccg
    15301 gttatcgcgg taacggcgca tgcaatggcc gggcaaaaag agaagctgct tggcgcaggg
    15361 atgagcgatt atctggcgaa accgattgaa gaagagcgat tgcataattt gttgttgcgc
    15421 tacaagcctg gctgcggtat ttcgtctcgc gtcgtgacgc ccgaagtcaa cgaaattgtg
    15481 gtgaacccga atgcgaccct cgactggcaa ctggcactac gccaggcagc aggaaaaacc
    15541 gatttagcgc gcgatatgct gcaaatgtta ctcgatttcc tgcctgaagt tcgcaacaaa
    15601 gttgaggaac agctggttgg agaaaacccg gaaggcctgg ttgatttgat tcataaactg
    15661 catggcagtt gcggctatag cggtgtgccg cgtatgaaga atctctgcca acttatcgaa
    15721 caacagctac gtagcggtac taaagaagaa gatttggaac cggagctgct ggaactgttg
    15781 gacgagatgg ataatgtcgc gcgcgaagcc agcaaaattc tcgggtaacg gacgtcttgt
    15841 cgagtagcaa tcggcttttt atgacgctgt tttcagattg gtgacaaagt gcctgtgttt
    15901 atgccggatg cggcgttaac gccttatccg gcctacaaaa ccaattaaat tcaataaatt
    15961 aaaaagcatg taggcctgat aagcgtagcg catcaggcaa ttttgtgttt ggcatcagtc
    16021 tcaaaccggc tccagataga gccggttttg gttttttgtc ttaacgcacc atgcacgggc
    16081 gcttgttatc gaacgtccag ccaggaatca gatactgcat tcccatcgca tcgtcacgcg
    16141 cgccaagccc gtgtttctga tacagctcat gggctttcat cacttgatcc atatcgattt
    16201 ctacacccag ccccggtttt tctggcacct gtaccagccc gcctttgatc tcaaacggtt
    16261 ctttggtcag gcgctgatta ccttcctgcc agatccagtg cgtatcaata gcagtaattt
    16321 tacccggtgc agcggcggca acatgggtaa acatcgccag ggaaatatcg aagtggttgt
    16381 tagagtgtga accccaggtc aggccaaatt catggcacat ttgcgccaca cgtaccgaac
    16441 cttgcattgt ccagaaatgc ggatccgcca gcgggatatc aacggattgt aacgagagcg
    16501 tatggcccat ctgccgccag tcggtggcga tcatattggt tgcagtcagc aggccagtcg
    16561 cgcgacggaa ctctgccatc acttcacgcc cggagaaacc ttgctccgca ccacacggat
    16621 cttctgcata agccagcgaa cctttcaggt atttgccgat tttaatcgct tcgttcagcg
    16681 accaggcacc gttaggatcg agcgtaatac gcgcctgcgg gaagcgttgc gccagtgcca
    16741 caatagactc ggcctcttct tccccggcca gtacaccgcc tttcagtttg aaatcgttga
    16801 agccatattt ttcatacgcc gcttccgcca ggcgcaccac cgcatccggc gtcatcgctt
    16861 cttcatgacg caggcgatac cagtcgcatg agtcatccgg ctggctttga tacggcagag
    16921 gtgtggcttt gcgattaccg acgaagaaca gataaccgag catttcgact tcgctacgct
    16981 gttgaccatc gcccagcagg gatgccacgt taacccccag atgctgcccc agcagatcca
    17041 gcattgccgc ttctatcccg gtaactacat gaatagtggt acgcaaatca aatgtctgca
    17101 aaccgcgccc gccggcatca cgatcggcaa aagtattacg caccagcgtc agaacgtttt
    17161 tgtattcacc cagcgtttta cccactacca gcggaattgc atcttccagc gttttacgga
    17221 ttttctcgcc gccgggaatt tcccctacgc cagtgtgacc agaattatct ttgataatca
    17281 caatattacg cgtgaagaac ggtgcgtgtg caccactcag attcatcagc atactgtcat
    17341 ggcccgccac cgggatgacc tgcatttcag taacaacagg cgtcgtaaat tgagaactca
    17401 tatttatgtc ctttttcaga atcaatgacg gccgaaaacg ggacgtttac ggtcaaaggt
    17461 ccagccgggg atcaggtact gcatcgggcc tgcgtcgtta cgcgcaccgc caggcagacg
    17521 tttataggcc tcatgtgcct tttgtacctg ttcccagtcc agttccacgc ccagaccggg
    17581 cgcatcagga acggcaattt ttccgttttt aatctccagc ggattttggg tcaggcgaca
    17641 atcgccctcc tgccaaatcc agtgggtatc gatagcggta ggattacccg gtgccgccgc
    17701 gcccacatgg gtaaacatcg ccagagagat atcgaaatgg ttgttggaat ggcagcccca
    17761 ggtcagcccc cagtcgtcgc aaagctgcgc cactcggact gcaccggaaa gcgtccagaa
    17821 gtgcggatcg gcaagtggaa tatctaccgc attgagcatc accgcatgac ccatttcgcg
    17881 ccagttggtg gcgatcatat tagtcgcgac gggcaagccg gtcgcccgcc gaaactccgc
    17941 catcacttca cgtccggaga agccctgttc tgcgccgcat ggatcttcgg cataggtaag
    18001 aacatcattc agccctttgc acagagaaat ggcttcatca agcagccatg caccgttggg
    18061 atcaacggta atccgcgcat ccgggaagcg tttcttcaat gcacgaacag tgtcgatttc
    18121 ttgctcgcca ggtaacacgc cgcccttaag tttgaaatct ttaaagccgt agcgatcctg
    18181 tgaggcttcc gccagacgca caacggcttc gctgttcatc gctttctgat ggcgcaactg
    18241 ataccactca tggttgcccg gcgtattttc cagataagga agatcggttt tggtccgatc
    18301 accgatataa aacagataac cgaggacggt aatagcatcg cgttgcttgc ctggccctaa
    18361 cagttcgcag accggaacat tcagcgcctt acctagcagg tcaagcaagg cggcttccag
    18421 cgccgccacg gcgttaacgc gcaattcaaa ggtccaggcc cctttaccga aggtatcaaa
    18481 atcggctgct tgattacctt tatgcacctg ctgaaccact ttattcaggc gcgcaacttc
    18541 ctagcccagc accatcggaa tagcatcgac caatgtctga taaatcacct ctccgcctgg
    18601 cgcttcacca atgccggtat gcccggcgtt atcggtgagt accacaatat tgcgagtgaa
    18661 atatgcgtta tgcgccccac caatattaag caacatgctg tcgtacccgg cgaccggaat
    18721 gactttcata tcagtaataa caggactgga ttgtgtcgtc atcttattgc cccacgacag
    18781 gtttcagctc gatacgcttg atatcgccca ccagcaccag gtagctcagt accgcgatta
    18841 aggcatgaac accaacataa atcagcgccc cattaaacga gccagtcgtg ccaacgatat
    18901 aaccaattgc gattggcgtg acgatgccag aaatgttacc gaacatgttg aacaggccac
    18961 cggaaagacc gctgatctct tttggcgcgg tatctgccat tactgcccaa cccagcgccc
    19021 caatgccctt accgaagaag gccagcgcca taaagccgat gatcatccac tcaacgttga
    19081 cgtagttgca gaacaccatc accatcgaca gcaacatgcc cattacgatc ggtgttttac
    19141 gcgcaatgtt cagcgatccc gtgcggcgca tcagccagtc ggaaataatc ccacccagca
    19201 caccaccgat aaaaccacaa accgccggaa cggaagccac aaagcccgct ttcagaatcg
    19261 acatcccacg cgcctgcacc agataaaccg gaaaccaggt aatgaaaaag taagtcaggg
    19321 cgttgataca gtactgaccg atataaacgc cgatcatcat ccgtgacccg agcagttgtt
    19381 tgatctgccc ccacttcacg ctgaacggca ctttaacttt ggtgttttgc tgatccatat
    19441 tgatcagcgc gccacccgcg gcgatgtact ccagctcttt ctgatttacc cccggatgtt
    19501 gatttggctc gtggatgact ttcaaccaga taaagctgat ggcaatgccc agaccgccca
    19561 taaagaagaa gacgtgtgac cagcccactt catgcgtcag ccagcccata atcggcgcga
    19621 agatcaccgt tgcgaagtat tgagcggagt taaaaatcga caccgccgtt cccctttcct
    19681 gcgccggaaa ccaggccgca acaatgcgac tgttgccggg gaaagatggc gcttcagcaa
    19741 gcccgaccag gaagcgcaga gtaaacaggg caacgataat gccgaatcca ctaaagatat
    19801 cgacgaagcc ttgcagcaag gtaaacatcg accagataaa gatcgaccag aagtagacgc
    19861 gttttgaacc aaaacgatcc agcaaccaac caccagggat ctgcccgata acataagccc
    19921 atgagaaggc agagaacaca tagcccattc ccacgggatc aaggccgata tctttggcca
    19981 tttccgaacc ggcaatagaa agcgtagcgc ggtcgccgta gttgaaggat gtgacgataa
    20041 acaacatcac cactatccag taacgagcat tcgtgcgttt ttccacactg ctcgcagcct
    20101 gacttaaaga actcattgtt gcactcctga aaattcgcgt tagccacgct cactctggac
    20161 tgcgacatcg ccaggaaatc agaggtgacg tagggtgttt tttgccgttt ttataggtcg
    20221 ttcgccgaat acggcgcgtg tttatatctg gcaatagcag tataaaaagc gcgccatagc
    20281 ggctcaccgt gcaacaacac aacattaatg cgttcaatga ggcccgattt tggcattagc
    20341 cctggacggt ggaatccact tcacggaaat gaaaacaaga acaagaaagg aagggttaaa
    20401 acgaagaaat aaagaagagt atgaaatgga tcgcttgact ccaggcaaac gccagtaaaa
    20461 atccgcgcta tgaagcagtt tttactggca tttgcctgaa aagattcgat tcagcaccgc
    20521 taaaacgaca tttaccgctc gctgaacata tcaggacaac agcgtgcccc actgttcgac
    20581 ccacggattt gattccgttt ccggttccgg gttctcactg gcgtcaatca acagcatttc
    20641 gccaacccgc tgagcgctct gttcctgcag caaggcatcg aactgtttgc cgccattgca
    20701 gaaattcaca taactactgt cgccgagcgc aatcacgcca taacgcagat tcggctggaa
    20761 gcccagacta tctttgattc cctgaaagag cggcacaatg ctatcaggaa ggtcgccctg
    20821 cccggtcgtg gacgtaacca ccagaacata cttatcctga tagggcagcc agtcgcttaa
    20881 ttcaggatct tcaaataccg ttgctttgtg gccctgtgcg gtcagaattg cttctgcttc
    20941 ttccgccact aacagcgaat ttccgtacat ggtgccgaca aaaataccaa tttccgccat
    21001 accgttttct ccctggatta ggaacttctc tctccatcct gacccgatgg cgcactgaac
    21061 tcaacccttt cattttcagg aagcagaccg cgccagccaa attgtgataa cgcctgcatc
    21121 caggtgtcgt ccaggcccgc gtgaatagtc agcggctcac cagtaaaagg atgcgtcaat
    21181 gacaactgac tggcatgcag cattaaccgc tgaagaccaa aatgctcagc accgctgcga
    21241 ttctggcgta aatcgccatg tttgctatcg ccaataatcg gatgacgcaa atgggcaaga
    21301 tgtcggcgaa gctgatgttt gcgtccggtt ttcggctcca gttccaccag gccgtagcgc
    21361 gtggtcgggt aacgtccggt cgctaccggc atttctacgg tcgccagacc gcgataatgc
    21421 gtcactgctg gctgcgggcc tttatcttcg cgggcaaatt tatcagcgat tttgtccagt
    21481 tcttccacca gtggataatc cagcaccgct tcttccatca accagccgcg cacaatcgca
    21541 tggtaacgtt tctggatttg gtgctgttca aactgttgtg ccagcagccg tccggcctcg
    21601 ctggataatc ccatcaacaa cacaccagaa gtgggtcggt ccagacgatg agcagtaaaa
    21661 acatgctggc ctatctggtc acgcacggtt tgcatgacca ctactttctc gtcgcgatcc
    21721 agccagctac ggtgaaccag ccagccggag ggtttattta ccgcaaccag ccattcatcc
    21781 tgatagagta tttccagcat tagctcgcat catccgcaaa aagagcatcc agtttttcca
    21841 actcagccag aataagcgcg cgttgcggat ggtccgtcgc cagcgccatt tcataatagg
    21901 gtgcaacggc aaaagcgccc ggtaacggct ggttgttatt taacaaatcg tgcattcgcg
    21961 ggatcagcac ccactgcaac cactccagtg gttccatggt gtccataaag aacggttggg
    22021 tactattaaa ttgatgcggc tggggttcat cgtttcgcca gtgctgatgt tcacgcagta
    22081 acgcttcaag cgcctgcaac tggagacgaa cgcggtcatg agtggtcata aagcaacctc
    22141 aataagaaaa actgccgcga aggatagcag gaataaaaaa agggagcact gtattcacag
    22201 tgctcccggt tcgtttcgca gcattccagc tacttttgtt gctccctgct catccttgac
    22261 aacttttcct ctggccatgc ggccaatcgt tcatcctgaa ctattgcttc ctgctcacac
    22321 caccccgatg tgatacttca tcctgaagtg tcccaggcct tcctgaccca ccgaatcatc
    22381 ctgaccggtt ctcattctcc atcctggagg tgtcctttaa cgcgtcctgc gtcatcctct
    22441 tcgcttcatc cagaagcctt tccctgaaac accatcctgg tgtgtccttc agaagtgtca
    22501 tcatcctgat gttcacttcg ttgtctgact ccctgtcgac gaagatagaa tcgtcttttt
    22561 cgcgaactct tacaaggcgc ttacagacaa tgccttaagg aaaatttcat atgaaagtta
    22621 ggaattaaca agcaacatgt tgtttttaaa tgaaaataac gattcttatc tttttaagta
    22681 ttcgcttatt tcctggcgat ctcctacaga gtctgtaaga gatctctcac aaccagatgg
    22741 ggtatttgga ttacagaaga ggcttaagtt gattaaggaa ttccgcaaga tttggggcta
    22801 aatgcgtccg tttacgtgtt cccagcgtct ctttacacac ttcaccggag agattacaca
    22861 ccgagatgac ctctaactct tcttccagcg tcgcgataaa tagcgtgggt ggcagtttta
    22921 gccttttctg agtcaccaga tgaccaatca gattttcctg cacccgacgg aagtcgtctt
    22981 cactccaggt ttgcagcaat gtcagtttga tatcgccaaa ctgcgcgtgc atatccccgg
    23041 caaactgagt cgtataaaag gtatgaattg tgggttgtat cacaatatca aaagcgcgtt
    23101 caaccgcgtt tacattttgt tccccggtaa acggctgggg ttgccagtat acggcatctt
    23161 cagtagttga aataatgcac ggtgaaggaa cgccgtacag ttcctcactt aacggccagc
    23221 ttttatgctc ttcatgccac gcatcgcagt agcgtgcagt aaaatctttc agggcttgtg
    23281 cggtcaaatc gtccactaat ttctctcttc acttaaacca gatacacttg tcgtttagtt
    23341 tatctggttt atgacggtga aacatgtctt cttatgcaaa ccatcaggca cttgcgggcc
    23401 tgactcttgg aaaatcaacc gattaccggg atacctatga cgccagccta ctgcaaggcg
    23461 ttccacgcag cctgaatcgc gacccgctgg gtctgaaagc ggataacctg ccttttcaag
    23521 gtacggatat ctggacgctg tatgaacttt cctggctgaa tgcgaaaggt ttgccgcagg
    23581 tcgctgtcgg tcatgttgaa cttgattaca ccagcgtaaa tctgattgag tcgaagagtt
    23641 ttaagctcta tctcaacagt tttaaccaga cgcgttttaa taactgggat gaggtgcgcc
    23701 agacgctgga gcgcgactta agcacttgcg ctcagggtga ggttagcgtg gcgttatatc
    23761 gtcttgatga actggaaggc cagccgatag gtcatttcaa tggcacttgc attgatgacc
    23821 aggatatcac tatcgataac tatgaattca ctactgacta tctggagaat gccaccagtg
    23881 gtgaaaaagt agtggaagag acgctcgtca gccacctgct gaaatcaaac tgcctgatca
    23941 cccatcaacc agattggggt tcgatccaaa ttcagtatcg tggacgccaa attgacagag
    24001 aaaaactgct gcgttacctg gtctcattcc gtcatcacaa cgagttccac gaacagtgcg
    24061 tggaacgcat ctttaatgac ctgttacgct tctgccagcc agagaaattg agcgtttacg
    24121 cacgttatac ccgtcgtggc ggtctggaca ttaacccgtg gcgcagtaat agcgattttg
    24181 tcccatcgac cacaagactg gttcggcaat aaattttttc tcaattttgc gtgctggatt
    24241 cacgcagaag gttgtgaaag gtcatcaggc agggctattg taatcaaagg gaatgacgat
    24301 attcgtccca taaggagttt ttcttgatta cacatattag cccgcttggc tccatggata
    24361 tgttgtcgca gctggaagtg gatatgctta aacgcaccgc cagcagcgac ctctatcaac
    24421 tgtttcgcaa ctgttcactt gccgtactga actccggtag tttgaccgat aacagcaaag
    24481 aattgctgtc tcgttttgaa aatttcgata ttaacgtctt gcgccgtgaa cgcggcgtaa
    24541 agctggaact gattaatccc ccggaagagg cttttgtcga tgggcgaatt attcgcgctt
    24601 tgcaggccaa cttgttcgcg gttctgcgag acattctctt cgtttacggg caaatccata
    24661 ataccgttcg ttttcccaac ctgaatctcg acaactccgt ccacatcact aacctggtct
    24721 tttccatctt gcgtaacgct cgcgcgctgc atgtgggtga agcgccaaat atggtggtct
    24781 gctggggcgg tcactcaatt aacgaaaatg agtatttgta tgcccgtcgc gtcggaaacc
    24841 agctgggcct gcgtgagctg aatatctgca ccggctgtgg tccgggagcg atggaagcgc
    24901 cgatgaaagg tgctgcggtc ggacacgcgc agcagcgtta caaagacagt cgttttattg
    24961 gtatgacaga gccgtcgatt atcgccgctg aaccgcctaa cccgctggtc aacgaattga
    25021 tcatcatgcc ggatatcgaa aaacgtctgg aagcgtttgt ccgtatcgct cacggcatca
    25081 ttatcttccc tggcggtgtg ggtacggcag aagagttgct gtatttgctg ggaattttaa
    25141 tgaacccggc caacaaagat caggttttac cattgatcct caccggcccg aaagagagcg
    25201 ccgactactt ccgcgtactg gacgagtttg tcgtacatac gctgggcgaa aacgcgcgcc
    25261 gccattaccg cataatcatt gatgacgccg ctgaagtcgc ccgtcagatg aaaaaatcga
    25321 tgccgctggt gaaagaaaat cgccgtgata caggcgatgc ctacagcttt aactggtcaa
    25381 tgcgcattgc gccagatttg caaatgccat ttgagccgtc tcacgagaat atggctaatc
    25441 tgaagcttta cccggatcaa cctgttgaag tgctggctgc cgatctgcgc cgtgcgttct
    25501 ccggtattgt ggcgggtaac gtaaaagaag tcggtattcg cgccattgaa gagtttggtc
    25561 cttacaaaat caacggcgat aaagagatta tgcgtcgtat ggacgacctg ctacagggtt
    25621 ttgttgccca gcatcgtatg aagttgccag gctcagccta catcccttgc tacgaaatct
    25681 gcacgtaatc tcccctcccc cggtgacgtt tgttgccggg ttttctcgtt tttggtcact
    25741 tactcatcaa ctcatttcat ttgttatatg aatgtttctt accaccctca cgcgacaaat
    25801 atcatcacag ttaatatgtc atacaattta tattgcaacg caaacgtttc cctattttca
    25861 taaaccgtat tttatcgcta tgaaaaagaa attatcgcca tgattaacta aaagtattga
    25921 ttttttcagt tcaacctaca tatattgcgc gccccggaag aagtcagatg tcgtttaatg
    25981 ggcaaatatt gcccttaaat tctcttttac ttttgattta cagagtaaag cgttgggata
    26041 atctatcttc caagtagatt attgtatttg ggatcaagat cactgataga tacataactt
    26101 gtgtgtatct ttccgccctc aaattattac ggcggtaaat gattaagcca tcgccgatag
    26161 acagatttca tttttacggt caggcacctt cccgggctga actggctaaa agctgaatta
    26221 tttgcattcc tccaggagaa atagatggaa acgactcaaa ccagcacgat tgcgtcgaaa
    26281 gactctcgta gtgcctggcg caagacagac accatgtgga tgctgggcct ttacggcacg
    26341 gcaatcggcg cgggcgtgct gttcctgcca atcaacgccg gtgttggcgg tatgatcccg
    26401 ctgatcatca tggctatcct tgcgttcccg atgacgtttt ttgctcaccg cggcctgact
    26461 cgcttcgtac tgtctggtaa aaacccgggc gaagacatta ccgaggttgt agaagaacac
    26521 tttggtattg gcgcaggtaa actgattacc ctgctctact tcttcgctat ctacccgatc
    26581 ctgctggttt atagcgtggc aatcaccaat acagttgaaa gcttcatgtc tcaccagctg
    26641 ggtatgacac caccgccgcg tgcgattctg tcgctgatcc tgatcgtggg tatgatgacc
    26701 atcgttcgct ttggtgaaca gatgatcgtt aaagcgatga gtattctggt attcccgttt
    26761 gttggcgtac tgatgctgct ggctctgtac ctgatcccgc agtggaacgg cgcagcactg
    26821 gaaacgctgt ctctggacac tgcatctgca accggaaacg gtctgtggat gaccctgtgg
    26881 ctggcaattc cggtaatggt gttctcgttc aaccactctc cgatcatctc ttctttcgcc
    26941 gttgcgaagc gtgaagagta cggcgatatg gcagaacaga aatgctcgaa gatcctggca
    27001 ttcgcacaca tcatgatggt gctgaccgta atgttcttcg tcttcagctg cgtactgagc
    27061 ctgactccgg cagacctggc tgcggctaaa gagcagaaca tctcgattct gtcttacctg
    27121 gctaaccact ttaacgcacc ggttatcgcg tggatggctc cgattatcgc gattatcgct
    27181 atcaccaaat ccttcctcgg ccactacctg ggcgcacgtg aaggcttcaa cggtatggtg
    27241 attaaatctc tgcgtggtaa aggtaagtct atcgaaatca acaagctgaa ccgtatcact
    27301 gcgctgttca tgctggtaac gacctggatt gttgccaccc tgaacccgag catcctgggt
    27361 atgattgaaa ccctgggcgg cccaatcatc gcgatgatcc tgttcctgat gccgatgtac
    27421 gcaattcaga aagtaccggc aatgcgtaag tacagcggtc acatcagcaa cgtattcgtt
    27481 gtcgtgatgg gtctgattgc aatctccgca atcttctact ctctgttcag ctaagtcctt
    27541 tcgcgccgct ttcgggcggc gcttcctccg ttttaacgcg atgtatttcc tatgattagc
    27601 gtattcgata ttttcaaaat cggcattggc ccttccagtt ctcataccgt tggaccaatg
    27661 aaagcgggta aacaatttac cgacgatctg attgcccgta acctgcttaa agacgtgacc
    27721 cgcgtggtgg ttgacgtgta cggctcgctc tctctgactg gtaaaggcca ccacactgat
    27781 atcgccatta ttatgggcct ggcgggtaac ctgccggata ccgtggatat cgattccatc
    27841 cctggtttta ttcaggatgt gaatactcat ggtcgcctga tgctggcaaa cggtcagcat
    27901 gaagtggagt tcccggttga tcagtgcatg aacttccatg ccgacaacct ttctctgcat
    27961 gaaaacggta tgcgcattac cgcgctggcg ggtgataaag tcgtttacag tcagacttac
    28021 tactctattg gcggtggctt tatcgttgat gaagagcatt ttggccagca ggatagcgca
    28081 ccggttgaag ttccttatcc gtacagttca gcagccgatc tgcaaaaaca ttgtcaggaa
    28141 accgggctgt cactctctgg cctgatgatg aaaaacgagc tggcgctgca cagcaaagaa
    28201 gagctggaac agcacctggc gaacgtctgg gaagtgatgt gtggcggtat tgagcgcggt
    28261 atttccaccg aaggtgtgtt acctggcaaa ctgcgtgttc cacgccgtgc tgcggcacta
    28321 cgccggatgc tggtcagcca ggataaaacc accactgacc cgatggcggt tgttgactgg
    28381 atcaacatgt ttgcactggc agtgaacgaa gagaacgctg ctggcggtcg cgtggtgact
    28441 gcgccgacta acggtgcgtg cgggattatc ccggcagtgc tggcgtacta cgataagttt
    28501 atccgcgaag tgaacgctaa ctcactggct cgttacctgc tggtagccag cgccattggt
    28561 tctctttata agatgaacgc gtcgatttct ggtgccgaag tgggttgcca gggtgaagtt
    28621 ggtgtggcgt gctcaatggc ggcggctggt ctggcagaac tgttaggtgc aagcccggcg
    28681 caggtgtgca tcgcggcgga aatcgccatg gagcacaacc tcggtctgac gtgtgacccg
    28741 gtcgccggac aggtacaggt gccatgcatc gagcgtaacg ccattgcggc agtaaaagcg
    28801 gtgaacgccg cacgtatggc gctgcgccgt accagcgagc cgcgcgtctg cctcgataaa
    28861 gttatcgaaa ccatgtacga aacaggtaaa gatatgaacg ccaagtaccg cgaaacctct
    28921 cgcggcggcc tggcaatgaa gatcgttgcc tgcgattaat cgctctccag aggcctcgtt
    28981 ttgcgaggcc tcttcccgat ttctcatcca gccatagcct gttccagtat cgaatgttac
    29041 ccttatcgcc tgatctttaa gggggttatc gtggctgttc atttgcttat tgtcgatgca
    29101 ctgaatctta ttcgtcgcat tcatgccgtt caggggtcgc cctgtgtcga aacctgccag
    29161 catgcgctcg atcagctcat tatgcacagc cagccaaccc acgcggtcgc cgtttttgac
    29221 gatgaaaacc gcagtagcgg ctggcgtcat cagcgtttac cagattacaa agcgggtcga
    29281 ccgccaatgc cggaagagtt gcacgacgag atgcctgcat tacgcgccgc ctttgagcaa
    29341 cgcggcgtcc cgtgctggtc aaccagcggc aacgaagccg atgacttagc cgccacgctg
    29401 gcggtcaaag tgacacaggc cgggcatcag gcaacgattg tttcgacaga taaaggctac
    29461 tgtcagttac tttcaccgac attacgtatt cgtgattact tccagaaacg ttggctggat
    29521 gcgccattta tcgataaaga atttggcgtt caaccgcagc agttgcccga ttactgggga
    29581 cttgcgggga tcagcagttc aaaggtaccg ggtgttgcgg gaatcggacc aaaaagcgcc
    29641 acgcagctgc tggtcgagtt tcagagtctg gaagggatat atgagaatct ggatgcggtt
    29701 gccgaaaagt ggcgcaaaaa attagaaacc cataaagaga tggcgtttct gtgccgcgat
    29761 attgcccgct tacaaaccga tttgcatatc gacggcaatt tacagcaatt gcggttggta
    29821 cggtaacggc gtgccggata cgccgcaaac gtcgcatccg gcattatcac atcagcgcat
    29881 ttaccaggcg gtatggtaaa gctctacaat atcctcaagc gttgcttcac gcgggttgcc
    29941 accggtacaa acatcatcca gtgccgcctg cgccagtgcc ggaatgtctt ccttgcgtac
    30001 accaacatca cgcaaatgtg gcggaatacc gacatcacgg ttgagagcaa acaccgcttc
    30061 aacagcggca ttacgcgcct cttccaggct cataccttcc actttcacgc ccataacgcg
    30121 cgcgatatcg cggtacttct caccggtaaa gtcagcgtta taacgcatga catgcggtaa
    30181 caggatggcg tttgcaacac cgtgtggagt gttataaaac gcgcccagtg gatgcgccat
    30241 accatgcacc aaccctaacc ccacattcga gaagcccata cccgcaacat actgcccgag
    30301 cgccatttct tctccggcat ccttatcacc agcaaccgat cctcgcagcg ccccagcaat
    30361 gatttcaatc gctttaatgt gcagtgcatc ggttagcgcc cacgcgccac gggtaatata
    30421 cccctcaata gcatgagtga gcgcatcgac acccgtcgca gctttcagcg ctggaggcat
    30481 accatccatc atgtcagcgt caataaacgc cacctgcggg atatcatgcg gatcaacgca
    30541 aacaaacttg cgccgttttt cttcgtcagt gatcacgtag ttaatggtca cttctgccgc
    30601 agtgcctgct gtggtgggga ttgccagaat cggtacactg ggtttattgg tcggggaaag
    30661 tccttccagg ctacgcacat cggcaaactc cgggttgttg ctgataatgc caatcgcttt
    30721 acaagtatcc tgtggagaac caccaccaat agcgatcagg taatccgcgc cgctattctg
    30781 gaatacaccg agcccttctt tgacgacagt aattgttggg ttgggcacta cgccgtcgta
    30841 aatcgcccat gccagccctg cagcatccat cttatcggtc actttcgcca ccacgccgca
    30901 ttgcaccagc gttttatcgg tgacgatcag cgccttctga taaccacggc gtttcacctc
    30961 atcggttaaa gccccaacag caccccgacc aaaccatgcc gtttcgttca gaatcattct
    31021 gttagccatc atccttctcc ttgttgcttt acgaaattac tcttcaatcc gtaacccata
    31081 ggttttgaat ttctccagca ctacggcaat ctcttcatcg ctcagcactg gcaccgggtc
    31141 cgtaatcgcc agggtcgtca ggtaaagttg cgccagcact tcaacttcat gcgccagcca
    31201 taacgctttt tccagattca cctcacaagc gataagccca tgatgttgta acaaagttgc
    31261 cttacgattt ttgagagcca gcgcaacatg ttcagaaagt tcgcgtgttc caaaggtcgc
    31321 ataaggcgcg caaggaatag aattaccgcc agccgccgca atcatgtagt gaatagcggg
    31381 gatcggtcgg ttaagaatgg aaactgctgt gcaatgaacg gcatgattgt gaacaaccgc
    31441 gttggcatcc ggtctgcttt gataggccgc catatggaaa cgccactcgc ttgaggggag
    31501 ctttccttcc tcatgtttac cgttgccatc aataaagaca atatgcgact ccgtcagttt
    31561 ttcatatgga atgcctgtag gcgtaatcag catcccatcc tgataacgta cactgacgtt
    31621 ccccgctgtc ccctggttca gtcccaggcg ggtcatttcc aggcaagtgt caataatctg
    31681 acgagcaagt ttatttcgtt ccattagcta cctctctctg attcaaaaca gggcaataat
    31741 gttgttcctt tcacactatt gaattagccg tttaattacc caccatcttc ttcctgatta
    31801 acaagaaaga aattcacaag cttatatttt gtgacctagt tcaactaatc acagtaaata
    31861 actgcaagtt ctctttttat aaccccatta aaaatgaccg ctcttaaaaa tatttatcaa
    31921 aacggtcatt tttctattca cccaagccag gaatgcccgt tttcggcata aacaattaat
    31981 acggtcatct ggtttgtgtt ttttatgatt tattttctga aacgggcatg aaatttcgat
    32041 tattaaagtg atggtagtca cataaagtca ccttccagct aataagtgtg accgccgtca
    32101 tattacagag cgttttttat ttgaaaatga atccatgagt tcatttcaga caggcaaata
    32161 ttcactgata tgaagcccga actcgctagt tttgcacttt tgaaaacata accgattacg
    32221 tgcttaagct tctgaaccta agaggatgct atgggaaaca catcaataca aacgcagagt
    32281 taccgtgcgg tagataaaga tgcagggcaa agcagaagtt acattattcc attcgcgctg
    32341 ctgtgctcac tgttttttct ttgggcggta gccaataacc ttaacgacat tttattacct
    32401 caattccagc aggcttttac actgacaaat ttccaggctg gcctgatcca atcggccttt
    32461 tactttggtt atttcattat cccaattcct gctgggatat tgatgaaaaa actcagttat
    32521 aaagcaggaa ttattaccgg attattttta tatgccttgg gtgctgcatt attctggccc
    32581 gccgcagaaa taatgaacta caccttattt ttagttggcc tatttattat tgcagccgga
    32641 ttaggttgtc tggaaactgc cgcaaaccct tttgttacgg tattagggcc ggaaagcagt
    32701 ggtcacttcc gcttaaatct tgcgcaaaca tttaactcat ttggcgcaat tatcgcggtt
    32761 gtctttgggc aaagtcttat tttgtctaac gtgccacatc aatcgcaaga cgttctcgat
    32821 aaaatgtctc cagagcaatt gagtgcgtat aaacacagcc tggtattatc ggtacagaca
    32881 ccttatatga tcatcgtggc tatcgtgtta ctggtcgccc tgctgatcat gctgacgaaa
    32941 ttcccggcat tgcagagtga taatcacagt gacgccaaac aaggatcgtt ctccgcatcg
    33001 ctttctcgcc tggcgcgtat tcgccactgg cgctgggcag tattagcgca attctgctac
    33061 gttggcgcac aaacggcctg ctggagctat ttgattcgct acgctgtaga agaaattcca
    33121 ggcatgactg caggctttgc cgctaactat ttaaccggaa ccatggtgtg cttctttatt
    33181 ggtcgtttca ccggtacctg gctcatcagt cgcttcgcac cacacaaagt cctggccgcc
    33241 tacgcattaa tcgctatggc actgtgcctg atctcagcct tcgctggcag tcatgtgggc
    33301 ttaatagccc tgactttatg cagcgccttt atgtcgattc agtacccaac aatcttctcg
    33361 ctgggcatta agaatctcgg ccaggacacc aaatacggtt cgtccttcat cgttatgacc
    33421 atcattggcg gcggtattgt cactccggtc atgggttttg tcagtgacgc ggcgggcaac
    33481 atccccactg ctgaactgat ccccgcactc tgcttcgcgg tcatctttat ctttgcccgt
    33541 ttccgttctc aaacggcaac taactgaaca tattttccga ataaagtgag gaatctgtaa
    33601 tgaacaaaat cagcttaccg aaaattggta tccgcccggt tattgacggt cgtcgcatgg
    33661 gtgttcgtga gtcgcttgaa gaacaaacaa tgaatatggc gaaagctacg gccgcactgc
    33721 tgaccgagaa actgcgccat gcctgcggag ctgccgtcga gtgtgtcatt tccgatacct
    33781 gtatcgcggg tatggctgaa gccgctgctt gcgaagaaaa attcagcagc cagaatgtag
    33841 gcctcaccat tacggtaacg ccttgctggt gctatggcag tgaaaccatc gacatggatc
    33901 caacccgccc gaaagccatt tggggcttta acggcactga acgtcccggc gctgtttacc
    33961 tggcagcggc tctggcagct cacagccaga aaggcatccc ggcattctcc atttacggtc
    34021 atgacgttca ggatgccgat gacacatcga ttcctgccga tgttgaagaa aaactgctgc
    34081 gctttgcccg cgccggtttg gccgtcgcca gcatgaaagg taaaagctat ctgtcgctgg
    34141 gcggcgtttc gatgggtatc gccggttcta ttgttgatca caacttcttt gaatcctggc
    34201 tgggaatgaa agtccaggcg gtggatatga ccgaactgcg tcgccgtatc gatcagaaga
    34261 tttacgacga agccgaactg gaaatggcac tggcctgggc tgataaaaac ttccgctatg
    34321 gcgaagatga aaataacaaa cagtatcaac gcaatgccga gcaaagccgc gcagttctgc
    34381 gcgaaagttt actgatggcg atgtgtatcc gcgacatgat gcaaggcaac agcaaactgg
    34441 ccgatattgg tcgcgtggaa gaatcacttg gctacaacgc catcgctgcg ggcttccagg
    34501 ggcaacgtca ctggaccgat caatatccca atggtgacac cgccgaagcg atcctcaaca
    34561 gttcatttga ctggaatggc gtgcgcgaac cctttgtcgt ggcgaccgaa aacgacagtc
    34621 ttaacggcgt ggcaatgcta atgggtcacc agctcaccgg caccgctcag gtatttgccg
    34681 atgtgcgtac ctactggtca ccagaagcaa ttgagcgtgt aacggggcat aaactggatg
    34741 gactggcaga acacggcatc atccatttga tcaactccgg ttctgctgcg ctggacggtt
    34801 cctgtaaaca acgcgacagc gaaggtaacc cgacgatgaa gccgcactgg gaaatctctc
    34861 agcaagaggc tgacgcttgc ctcgccgcta ccgaatggtg cccggcgatc cacgaatact
    34921 tccgtggcgg cggttactct tcccgcttcc ttaccgaagg cggcgtcccg ttcaccatga
    34981 ctcgtgtcaa catcatcaaa ggcctgggac cggtactgca aatcgcggaa ggctggagcg
    35041 tggaattgcc gaaggatgtg catgacatcc tcaacaaacg caccaactca acctggccaa
    35101 ccacctggtt tgcaccgcgc ctcaccggta aagggccgtt tacggatgtg tactcggtaa
    35161 tggcgaactg gggcgctaac catggggttc tgaccatcgg ccacgttggc gcagacttta
    35221 tcactctcgc ctccatgctg cgtatcccgg tatgtatgca caacgttgaa gagaccaaag
    35281 tgtatcgtcc ttctgcctgg gctgcgcacg gcatggatat tgaaggccag gattaccgcg
    35341 cttgccagaa ctacggtccg ttgtacaagc gttaatacat tactccctga tgtgatgccc
    35401 ggtcgctccg gctgccgggc ctgaacaagc aagagtggtt agccggataa gcaatgttat
    35461 ccggctatat tgcaggagcg atcatgaaac aagaagtgat cctggtactc gactgtggcg
    35521 cgaccaatgt cagggccatc gcggttaatc ggcagggaaa aattgttgcc cgcgcctcaa
    35581 cgcctaatgc cagcgatatc gcgatggaaa acaacacctg gcaccagtgg tctttagacg
    35641 ccattttgca acgctttgct gattgctgtc ggcaaatcaa tagtgaactg actgaatgcc
    35701 acatccgcgg tatcgccgtc accacctttg gtgtggatgg cgctctggta gataagcaag
    35761 gcaatctgct ctatccgatc attagctgga aatgtccgcg aacagcagcg gttatggaca
    35821 atattgaacg gttaatctcc gcacagcggt tgcaggctat ttctggcgtc ggagccttta
    35881 gtttcaatac gttatataag ttggtgtggt tgaaagaaaa tcatccacaa ctgctggaac
    35941 gcgcgcacgc ctggctcttt atttcgtcgc tgattaacca ccgtttaacc ggcgaattca
    36001 ctactgatat cacgatggcc ggaaccagcc agatgctgga tatccagcaa cgcgatttca
    36061 gtccgcaaat tttacaagcc accggtattc cacgccgact cttccctcgt ctggtggaag
    36121 cgggtgaaca gattggtacg ctacagaaca gcgccgcagc aatgctcggc ttacccgttg
    36181 gcataccggt gatttccgca ggtcacgata cccagttcgc cctttttggc gctggtgctg
    36241 aacaaaatga acccgtgctc tcttccggta catgggaaat tttaatggtt cgcagcgccc
    36301 aggttgatac ttcgctgtta agtcagtacg ccggttccac ctgcgaactg gatagccagg
    36361 cagggttgta taacccaggt atgcaatggc tggcatccgg tgtgctggaa tgggtgagaa
    36421 aactgttctg gacggctgaa acaccctggc aaatgttgat tgaagaggct cgtctgatcg
    36481 cgcctggcgc ggatggcgtg aaaatgcagt gtgatttatt gtcgtgtcag aacgctggct
    36541 ggcaaggagt gacgcttaat accacgcggg ggcatttcta tcgcgcggcg ctggaagggt
    36601 taactgcgca attacagcgc aatctacaga tgctggaaaa aatcgggcac tttaaggcct
    36661 ctgaattatt gttagtcggt ggaggaagtc gcaacacatt gtggaatcag attaaagcca
    36721 atatgcttga tattccgtta aaagttctcg acgacgccga aacgaccgtc gcaggagctg
    36781 cgctgttcgg ttggtatggc gtaggggaat ttaacagccc ggaagaagcc cgtgcacaga
    36841 ttcattatca gtaccgttat ttctacccgc aaactgaacc tgaatttata gaggaagtgt
    36901 gaaatgctga aaacaatttc gccgttaatt tctcccgaac tattgaaagt gctggcagag
    36961 atgggacatg gagatgaaat tattttttcc gatgctcact ttcccgccca ttcgatggga
    37021 ccgcaggtga tccgcgctga tggcctgttg gtgagcgact tgctccaggc gattatcccg
    37081 ttatttgagc tggacagtta tgcaccgccg ctggtgatga tggcagcggt agaaggtgac
    37141 actctcgatc ctgaagtaga acgacgttac cgtaatgcgc tttcactaca agccccgtgt
    37201 cctgacatca tccgcatcaa tcgttttgcg ttttatgaac gggcgcaaaa agcctttgcg
    37261 atcgttatca caggcgaacg agcgaagtac gggaatattc ttttaaaaaa aggggtaaca
    37321 ccgtaatctc ataccggtac gcccgcatga cgcgggcggt tatcgaatga tggggtgaaa
    37381 aatatgaaag cggcacgcca gcaagcgata gtcgacctgc tgctgaacca taccagcctg
    37441 accacggaag ctctctctga acagctaaag gtcagtaaag aaaccattcg tcgcgatctc
    37501 aatgaattac agacgcaggg taaaattctg cgcaatcatg gacgcgctaa atatatccac
    37561 cgtcaaaatc aagacagtgg cgatcccttt cacatcaggc tgaaaagcca ttatgcgcat
    37621 aaagcagata tcgcgcgcga ggcgctcgcg tggattgaag aagggatggt gatagcctta
    37681 gacgccagtt caacttgctg gtatctggca cgccagttgc ctgacatcaa cattcaggtc
    37741 ttcaccaata gccatccgat ttgccatgaa ctcggtaaac gcgaacgcat tcaactgatc
    37801 agttccggcg gcacacttga gcgcaaatat ggctgttacg tcaatccctc gctgatttcc
    37861 caacttaaat cgctggaaat cgatctgttt attttttctt gtgaagggat cgatagcagc
    37921 ggcgcactgt gggactccaa tgctatcaac gctgattaca aatcaatgct attaaaacgc
    37981 gccgcgcaat cgttgttatt gattgataaa agtaaattta atcgttcagg ggaagcccgc
    38041 atcgggcatc tggatgaggt aacgcacatt atttctgatg aacgccaggt tgcaacttct
    38101 ttggtaacag gctgacggaa agggtagcag gccggagacg acgccccggc cttgcctgtt
    38161 atcgctcgtc gcgacgacca cccaccgcag cccagatacg gcggacgtgc accgtcactt
    38221 cttcgcgatc gtgatacaac tgccgcgcct gaatctgagc atttatgcca tgttcatcaa
    38281 gctgtgcctg aatatacgcc agattgtgtg acacttcttc gtagcgtttt ttcatcggca
    38341 gtttgaggtt gaaaatggtt tcacgacacc agccattaac cagccactgc gccatcaatg
    38401 ccgcaacttt cgccggtttt tcaaccatat cgcataccat ccaggagata ttgctgcgcg
    38461 tcggacggaa tttgaaaccg tcttcccgca gccacgtcac ctgtccggta tccatcagac
    38521 tttgcgccat cgggccgttg tcgacggaat aaacccacat gttgcgcttc accagttggt
    38581 aggtccagcc gccagggcaa gcgcctaaat ccaccgccca catcccgttc gccaggcgtt
    38641 catcccactc atccgcagga ataaacacat gaaatgcctc ttccagtttg agcgtggaac
    38701 gactcggcgc atctgccgga aatttcaggc gcggaatgcc catatagaac ggcgaattat
    38761 tgttgctgta tgagtaaccg gtatagcagc agcctggtgc aataaagaat acatgcacaa
    38821 ccggacgctt cggcgtttca tagttcgcca gcaccccggc atcgcgcaag gcagcgcgta
    38881 gcggcacggt gaatttacgg cagaatttca gcaactcttt gctttcgttg gtatcggcaa
    38941 cttcaacacg cagttcaccg cccttctcta ctacgccctg caacatgccg acaatggggg
    39001 taatacgatc ttctggcggc aaatgctgca agagttcccc caccacaaac cactggcggg
    39061 caaaaattaa tgaactgaac ggcagctcac ggattaactt atcgccatca tcaggttgat
    39121 aacattcata aatgacataa cccgcattct ctttcacgcg ggcaaaaccg aaaatctccc
    39181 gctggccggc tttatcggta atttctgcgg cgcactcttt ttcaaagccc ggacggcaca
    39241 gcaatacaac cttattcatg acttacgccc ttgcgcttta aacggatagc accaactaac
    39301 attaacgccc agcccgcaag gaagctcacg ccgccaaccg gagtgacaaa cgcccacaaa
    39361 cgcagatggg acagtgccag gcaataaagg ctgccgctga acagcaccgt gcctaacgcg
    39421 aggaaaacgc tactccagta aaaccagata ctgatgcgac gctgcattgc caccgccaga
    39481 cctaagatcg ccagcgtatg aaacgcctgg tattcgaggc cggtctggat ccagcccatc
    39541 tcaacggccc ccatggtttt acttaacaca tgcgcgccaa aagcgcccag tgccacaaaa
    39601 atgaagccgc taatggcggc gaaaatcagc ataaaacggc tggtcatggt cgtaccctac
    39661 gtaaattatt gttcataacg aaagcggaat ttttcttgtt cagcagcggc tttcgccagg
    39721 atccattggc gaaaggcggc tattttaccc agttctgcct ggctgtcatg acaaaccaga
    39781 taaaaagcgt ttttactgac cagaacatca ttaaacgggc aaacaagacg tccggcttca
    39841 atttcagatt gcgccatcac gttatttgcc agcgccactc cctgcccgtg aatagccgct
    39901 tgcagcacca tggcgctatg gctaaaaatt ggcccttgct gaacgttgat atgatttaac
    39961 cccaattgac gagtatatgt ctgccagtca cggcgtgacg catcatgtaa taacgtatgt
    40021 ttagccagat cttccggggt cttcaagggt ttttcgccag tcagcagtag cggcgaacac
    40081 accggcaata aatattcggc gtacagtttt tccacccgta gccccggcca gttgccccga
    40141 ccataaaata tcgccacatc aacatcatcc gccagcttat cttcctgacg atcaaccgcc
    40201 tggattcgaa cgtcaattcc cggataagct gaattaaagc tggaaagtcg cggaaccaac
    40261 caatgaatgg cgaaactggg gagtaaactg accgtcaacg cccccttggc gctacgggcc
    40321 tggagtttac gcgtcgcttc ggttaattgc gaaaatatct ctttgatatc gaggaaatag
    40381 ctttgccctt cctcggtcag caggagtgaa cgattacggc ggcggaacag ttttagcccc
    40441 aaaaaatcct caagagactt gatttgatga cttactgcgg cctgggtcac aaaaagctct
    40501 tctgctgcgc gagtgaaact taaatggcgt gctgcggcat caaaaactcg taaggcattt
    40561 agcggtggta atcgtttaga catggctttt aaactttgat gttaaatgaa tttaacaatt
    40621 agatcacact gtgtaaccta ttagtttttt taatctgagc cattataaat tgtccgttga
    40681 gcttctacca gcaaatacct atagtggcgg cacttcctga gccggaacga aaagttttat
    40741 cggaatgcgt gttctggtga acttttggct tacggttgtg atgttgtgtt gttgtgtttg
    40801 caattggtct gcgattcaga ccatggtagc aaagctacct tttttcactt cctgtacatt
    40861 taccctgtct gtccatagtg attaatgtag caccgcctaa ttgcggtgct tttttttacc
    40921 ttgcgatcgc gaattactga tccagttcga ccatctcttt cacgtcggta cggttgatct
    40981 gctgtttgtt gccattagcg tctttatacg aaatcatacc ggtatcgtta tcagtctgtg
    41041 gtttgccgtc agagacgatg gtacgtccgt cattggtgtg catcacatag ttcgaaccgg
    41101 aacaggcgct cagggcaaaa gtcagcatac aggcagaaat aattgcggca gtctttttca
    41161 tcatcttctc cttaaagcga ttaataaatc ttaatagtat tgattaacag gctaaaatta
    41221 acgcctaaca ctattcagca tatgttactg acgcggcttc gccaggatat ccagataatt
    41281 ctgatggtta gcactctcct tgtatcaaag tgaattttgc gtcacgatcg gtgcatcaag
    41341 ccgaggagta ccatgaacgt ttttaatccc gcgcagtttc gcgcccagtt tcccgctcta
    41401 caggatgcgg gcgtctatct cgacagcgcc gcgaccgcgc ttaaacctga agccgtggtt
    41461 gaagccaccc gacagtttta cagtctgagc gccggaaacg tccatcgcag ccagtttgcc
    41521 gaagcccaac gcctgaccgc gcgttatgaa gctgcacgag agaaagtggc gcaattactg
    41581 aatgcaccgg atgataaaac tatcgtctgg acgcgcggca ccactgaatc catcaacatg
    41641 gtggcacaat gctatgcgcg tccgcgtctg caaccgggcg atgagattat tgtcagcgtg
    41701 gcagaacacc acgccaacct cgtcccctgg ctgatggtcg cccaacaaac tggagccaaa
    41761 gtggtcaaat tgccgcttaa tgcgcagcga ctgccagatg tcgatttgct gccggaactg
    41821 attactcccc gtagtcggat tctggcgttg ggtcagatgt cgaacgtaac tggcggttgc
    41881 ccggatctgg cgcgagcgat tacctttgct cattcagccg ggatagtggt gatagttgat
    41941 ggtgctcagg gggcggtgca tttccccgcg gatgttcagc aactggatat cgatttctat
    42001 gctttctctg gtcacaaact gtatggcccg acgggtatcg gcgtgctgta tggcaaatca
    42061 gaactgctgg aagcgatgtc gccctggctg ggcggcggca aaatggttca cgaagtgagt
    42121 tttgacggct tcacgactca atctgcgccg tggaaactgg aagcaggaac gccaaatgtg
    42181 gctggtgtca taggattaag cgcggcgctg gaatggctgg cagattacga tatcaaccag
    42241 gccgaaaact ggagccgtag cttagcaacg ctggcggaag atgcgctggc gaaacgtcca
    42301 ggctttcgtt cattccgctg ccaggattcc agcctgctgg cctttgattt tgctggcgtt
    42361 catcacagcg atatggtgac gctgctggcg gagtacggta ttgccttgcg ggccgggcaa
    42421 cattgcgctc agccgctact ggcagaatta ggcgtgaccg gcacactgcg cgcctctttt
    42481 gcgccatata atacaaagag tgatgtggat gcgctggtga atgccgttga ccgcgcgctg
    42541 gaattattgg tggattaatg acgaacccgc aattcgccgg acatccgttc ggcacaaccg
    42601 taaccgcaga aacgttacgc aataccttcg caccgttgac gcaatgggaa gataaatatc
    42661 gccagttgat catgctgggg aaacagcttc cggctttgcc agacgagtta aaagcgcagg
    42721 ctaaagagat tgccggatgc gaaaaccgcg tctggctggg atataccgtg gctgaaaacg
    42781 gtaaaatgca cttctttggc gacagcgaag gacgtatagt gcgcggcctc ctggcggtgc
    42841 tgttgactgc cgttgagggg aaaaccgccg ccgaattgca ggcacagtcg ccgctggcat
    42901 tgtttgatga gctgggattg cgtgcgcaac ttagcgcctc acgcagccag gggttaaatg
    42961 cgttaagcga ggcgattatc gctgcgacga agcaggttta agcacagaac cgggtcggat
    43021 aagacgtttg cgtcgcatcc gacctgattg tttacccctg acgcgccgct ttcgccatca
    43081 tcttcttcag cgcatgagaa accgcaacaa aaccaaaggt ggcggtcacc atcgttgccg
    43141 cgccaaatcc agatgcacaa tccatccgct ttggtccttc cgccgtggct ttcatcgcac
    43201 acaccgtacc gtctgactgc ggatacacca atgcttcggt agagaacacg caatccacac
    43261 cgagcttacc tttactgttt ttcactacgc caaaatcgct tttcaggcgc tcacgcaact
    43321 tcgccgccag cgggtcctga atcgttttcg ccagatcggt aacctgaatc tgcgttgggt
    43381 caatctgccc acccgcgcca ccggtcgtga ccagcgggat tttattgcgc cgacaataag
    43441 caatcagcgc cgctttgggc cgtacactat caatggcatc aatcacgtac gaataaccca
    43501 cgctcatatg ctgcgctacg ttatccggcg tcacgaaatc atccaccacc gttacacagc
    43561 actccgagtt aatctggcga atacgttccg ccatgacttc cgcttttgcc agcccaacgt
    43621 tatcgcgcag ggcatgaatt tgccgattgg tattggtgac gcacacatca tccatatcga
    43681 taagcgtgat tgcgccaatc cccgtgcgcg ccagcgcttc cgccgcccag gaaccgacac
    43741 cgccaatacc gaccacacaa atatgcgcgt cagcaaacag ttgcaacgct ttttcaccat
    43801 acagacgcgc tgtgccacca aaacgctgac gccatgcatc actaattacc acagacatag
    43861 cacctcagaa ttaaaaaggg cgaggttatc cctcacccta aacgcataaa tgcctgatgc
    43921 gctacgctta tcaggcctac ataactcctg caatattttg gatttgcatg atttgtaggc
    43981 cggataaggc gttcacgccg catccggcat gaacaacgcg cacttagtca ataatcagga
    44041 tgtgaggtta cccctcaccc tgtcatatcc gtaaaaacgg catacagaat atcacatcag
    44101 ccgctaaaga cgttacctgc gcccggggcg gttttcagca cccagacacg tccatagtgg
    44161 ttgtaccaac ctgcgcggtg tccggcttcc ggcccgatcc cttgataaat atcgaagtgt
    44221 tggcctttga ttgcgccacc gacatccaac gccaccatca gacgcaattc gtactgaccg
    44281 ttaaatttgc cgttattatc cagcagcggc acttctgcca gcaaggttgt acctggtggg
    44341 ataatggaac gatcagaggc aacagaagca cggccaacca gcggcaccgc acttgcccct
    44401 ttcaccggtg caaaagattg cggtttaaag aaaacgaaag acgggttctg ttccagcagc
    44461 tcgcgaacct cggcttcact gtgtgtttcg ccccaatgac gaatcgcctg catcgacata
    44521 tcttcttttt tcacttcgcc acggtcgatc agcaccttac caatgctgcg ataggcatga
    44581 ccgtttttcc ctgcatagct gaaaaagtta agcggactgc catcaccaaa gtcgatatac
    44641 ccactaccct gcacatccat aatgaaatta tccatcagag agttgctgta agcgaggatg
    44701 tacttatcgc tcaatgcacc cgcgtagatc tccgcacgag acggcagacg accacgtttt
    44761 ggcggcatac ggtaaatagg atactggaac tcgccctggc gggtatggcg cgcctgaatt
    44821 accggcgtgt aatagcctgt aaactgcacg ttaccatagt tgtcggcacc ttccatctgc
    44881 caggcatcaa tgccgaactg gcgcatattg cgggtatcac cgcctgcgcg cagccactct
    44941 tgcaccgcgt tataaacatt actctggttg ccatacagac gcggtgacga attacggata
    45001 tggttaattt gctcggcaaa atcaccggcg ttaatcggcg cgccaacggc atctggctgg
    45061 ttcaccagag agaaaggctg ggtaaatttc ccgtctttat attgctgtcc gcgatcggtt
    45121 ggtttggaag agcaggcggc aagcattgcc acaaccgtgc ccataagaag gtactttacc
    45181 caacgtcctt tcattgttct cttcttcagg ttaaaaaata aggcgcaacg aagataacaa
    45241 accaccgcgt ttaatgaaat gagcagcctc tccctgacgc aaattttgca caaaaaatag
    45301 gctttagtga tttgtttttg ttcaaaatca tgccagatcc gtgatcgggg taaaaaaaag
    45361 gttgcatgaa aacgcgagcg gagtatagtg cgcatccacg gacgcggggt ggagcagcct
    45421 ggtagctcgt cgggctcata acccgaaggt cgtcggttca aatccggccc ccgcaaccaa
    45481 ttattgaaca ccctaacggg tgtttttttg tttctggtct cctataaaaa agcgccattc
    45541 agcgcctttt tatcatcccc ttctcgccag ggtcgcccca tcggcaaaat acgctttaat
    45601 tcccgcaaga atagactccg caacttcctg ctggaaggtc gccgttttca gtttacgctc
    45661 ttcctcaacg ttactgataa acgccgtttc gaccagaatg gagggaatat ctggtgcctt
    45721 tagtacggca aacccggcct gttcaacttg atttttatgc agcttgttga ttttaccgag
    45781 cttattcagc accgctttac caaacttaag gctgtcagca atggtcagcg actgcaccat
    45841 atcgaacatg gtgtggtcga catagcggtc accgcttttg ctcacgccac caatcaagtc
    45901 ggaggcgttc tgggtttgtg ccagatattt tgccgcagta ctggttgcac ctttggttga
    45961 gagcgcaaac accgaggaac cgctcggctg gcgactggta aaggcgtcgg catggataga
    46021 gacaaacagg tcagcacgct gtttctgagc ttttgctacg cgcacttgca acggaatgaa
    46081 gatgtcttca ttgcgcgtca tgtacacctt catattgccc tctttctcga tcagagagcg
    46141 caaacggcga gctatttgca ataccacatc tttttcgcgc gttttgtatt tccccaccgc
    46201 accggagtct tcgccaccgt ggccagggtc aagcataatg acaatcggac gatcgcgccc
    46261 tgctttacct ggttgtggac cactttgcgc tggcggcacc tgcttttcga ggtcgccttt
    46321 gttgtaatcc tccagcagcg ccagcagcgg gtcctgcata tcctgtgcat tggccggata
    46381 gaggtccatc accagacgct ctttaaaccc ggcgaccggc gcaagggcaa acagctgcgg
    46441 ttttacgttt tgctttaatt caaaaaccat ccgtacggtt tgcgggtcaa attgcccgac
    46501 gcgcgccgac ttgatgaacg ggtcgtctgc acgaatttgt gccgccatcc ccttgagtac
    46561 cgagttcagg tttacatctt cgatatccac caccacgcgt tccgggttac tcaacgcgaa
    46621 ctgcttatat ttcagctgac gatttgattc taccgtcacg cgggtgtagc tggacgcagg
    46681 ccagacgcgc accgccacga cctggctgac cgcagccaga ctgacctgac ttacgctcaa
    46741 tagccacata gcacccgcgc cttgcagtaa acgacgacgg ctgattgcag tgttggatcc
    46801 tgacatgcct ctcccgagca aaagaaatct aagctgtgta acaagtaaac gactaatttg
    46861 accggaaact ttagcgaaag acgcataatc tgtcatctaa taaacggtaa acattctttt
    46921 tatattcacg ccattactga taaaaaagtc gctctcgcat aaaatttaca cttgcaccct
    46981 gcgaaaaaac agaataaaaa tacaataaat tcgaataata atgcaaagag gtgtgccgtg
    47041 gtaaaggaac gtaaaaccga gctggtcgag ggattccgcc attcggttcc ctatatcaat
    47101 acccaccggg gaaaaacgtt tgtcatcatg ctcggcggtg aagccattga gcatgagaat
    47161 ttctccagta tcgttaatga tatcgggttg ttgcacagcc tcggcatccg tctggtggtg
    47221 gtctatggcg cacgtccgca gatcgacgca aatctggctg cgcatcacca cgaaccgctg
    47281 tatcacaaga atatacgtgt gaccgacgcc aaaacactgg aactggtgaa gcaggctgcg
    47341 ggaacattgc aactggatat tactgctcgc ctgtcgatga gtctcaataa cacgccgcta
    47401 cagggcgcgc atatcaacgt cgtcagtggc aattttatta ttgcccagcc gctgggcgtc
    47461 gatgacggcg tggattactg ccatagcggg cgtatccggc ggattgatga agacgcgatc
    47521 catcgtcaac tggacagcgg tgcaatagtg ctaatggggc cggtcgctgt ttcagtcact
    47581 ggcgagagct ttaatctgac ctcggaagag attgccactc aactggccat caaactgaaa
    47641 gctgagaaga tgattggttt ttgctcttcc cagggcgtca ctaatgacga cggtgatatt
    47701 gtctccgaac ttttccctaa cgaagcgcaa gcgcgggtag aagcccagga agagaaaggc
    47761 gattacaact ccggtacggt gcgctttttg cgtggcgcag tgaaagcctg ccgcagcggc
    47821 gtgcgtcgct gtcatttaat cagttatcag gaagatggcg cgctgttgca agagttgttc
    47881 tcacgcgacg gtatcggtac gcagattgtg atggaaagcg ccgagcagat tcgtcgcgca
    47941 acaatcaacg atatcggcgg tattctggag ttgattcgcc cgctggagca gcaaggtatt
    48001 ctggtacgcc gttctcgcga gcagctggag atggaaatcg acaaattcac cattattcag
    48061 cgcgataaca cgactattgc ctgcgccgcg ctctatccgt tcccggaaga gaagattggg
    48121 gaaatggcct gtgtggcagt tcacccggat taccgcagct catcacgggg cgaggttctg
    48181 ctggaacgca ttgccgctca ggcgaagcag agcggcttaa gcaaattgtt tgtgctgacc
    48241 acgcgcagta ttcactggtt ccaggaacgt ggatttaccc cggtggatat tgatttattg
    48301 cccgaaagca aaaagcagtt gtacaactac cagcgtaaat ccaaagtgtt gatggcggat
    48361 ttagggtaag cgatgaaaat cgtcggatgc gacatgcgta acactcgtac gtcgcatccg
    48421 gcaattacgt ttattcccgt gaactaaaca acgccgccag accactgcgc cgctcagtac
    48481 gagtggcgat tgccgcactt aatatgcgct catcggcata cagcgacaga cggcgacgcg
    48541 cgcgggtcac cgcggtataa accagctctc gcgttactac cggcgtgcgt tggctcggta
    48601 aaatcaacgc cgcatggtcg aactccgatc cctgcgattt atgtaccgtc atcgcccacg
    48661 tagtttcgtg ctctggcaag cgactcggtt gcacagactt aatattgccg tccggcatcg
    48721 caaaccagac gcgcgtcccc tgcccgcgat ccagcgcaat accgatatcg ccattaaaca
    48781 acccaagcgc gctgtcatta cgggcaatca tcaccggtcg gccttcgtac caacgagagt
    48841 gcggattacg atgaattttg cgcttctgtt gcataaactg ctcaattcgc tcattcagtc
    48901 cagccacgcc aaatggccct tcccgcaggg cgcacaaaag ttggtactca ttgaacgcct
    48961 gaatgattaa atccggctcg gcacgcgctt gcagcagatc cagataacgt ccataacccg
    49021 caagagcttc ctcaagcatc gcaatataat cttcgccgct ctgtaaaagc cgtttttcaa
    49081 tatcagtaaa atcctgctga aaaacggttt tcactgccgt tttatcacca cggttgatcg
    49141 ccgcagctaa ctgaccaatg ccagaatcgc tgccgaaacg atagcttttt tgcagcaggc
    49201 agagactgtc gcgcaaagat gccgcttctg tgccagttcc tgccggaacg tgagttcccg
    49261 tcaggcggct tagctgcccg gcacgctcgg cggtaaagcc ggcattggca taagcgcaga
    49321 tatcgcccag cacagcccca gcctcaaccg aggccagttg atcacgatcg ccgagaaaga
    49381 tcactcgcgc atgatcgggc aaggcgtcga tcagtctcga catcataggc agatcgatca
    49441 ttgacgcttc atctaccacc agcacatcaa gatgcagcgg gttaccggca tgatgacgta
    49501 aacgctggct acccggctgc gcacccagca atcggtgcaa agtactggca tcttccggaa
    49561 tacgtttctt ttgttcatcg gtcagcggta actgtcgcaa agccttgccg agagattcgg
    49621 ttaagcgcgc ggcagcttta cccgttggtg cagccagacg gatacggcag cgttcgccgt
    49681 cggccatttg aattaacgct gccagcaact tcgctacggt ggtcgtttta ccggtgccag
    49741 ggccgccgga aatcaccgag atccgccgcg tcagcgccac tgctgccgcc actttttgcc
    49801 agttaatttc atcaccagtc ggaaagagtt tatccagggt ttgcgccaac aaggcttcat
    49861 ccacctcgat ggcatgattc acctcattga agaagcgcgc taccgtccgc tcgttacacc
    49921 acatgcgatt caaataaaga cgatcgccac agaggatcat cggcgtgggt tcatctcccc
    49981 ggctgaccgc ttgtgaagcc agcaagcatt cttcccaatt ttgtagctca ccgatttcac
    50041 tgacacaggt cgccaacagc ggatgcgact cctcattatt ttccagtcgt gaaagcggca
    50101 aacaaacgtg cccctctccg gcatcatgac ttaacagtgc cgccgcgagg gtgacggcag
    50161 gatgttcatc tcccgccacg gtcagggcaa actgcacgtc cagcgggcgt agctgtttgt
    50221 gctccacagc ttccagtaat tgcttttgca atttcattac gcctcctcca gagtcatacc
    50281 ggcaaacatc tcatccatca ggtcaatcaa cccggcgttg gggcgggttg tgtaaatccc
    50341 ttgttgcgga tgttctttat caacaccacg caggaacaga taaataacgc cgccaaagtg
    50401 gcgctcatag tcgtaatcag caatgcgatg gcgcagataa cggtgcagcg ccagggtata
    50461 aagctgatat tgcagatcat agcggtgtgc ctgcattgcc gctgccatag cctgttgggt
    50521 gtaagccgaa ctatcttcac ccaaccagtt ggatttatag tcgagcaggt aataacgccc
    50581 ttcgtggcgg aacaccaggt cgataaagcc ttttaacatg ccacgtgcct gcatgaactc
    50641 cagcggcggg cagccagcgg atagcgggtc aaactggcga attagtgcat caagctggct
    50701 ggcgatgagc ggttcgctaa tcggcaggta aaactccatc tccacctgtt tatcacggtc
    50761 ggaaagctga ctcaggctaa cacccgtttc attgagaggt gcctggagga cagccgtgat
    50821 ccactcggtc aacaccggtt cccactgcga ttcaaagccg ccgagttcca gtttttcctg
    50881 tacccagttc gggtcaaccg gctgggtaaa attgaggtct tcaaacaaac tgtgcaagaa
    50941 tgtccccggt gacgcaccgc gcgggaactg atgcggtgtt aacgtcggtt cttcaacgac
    51001 gctgaccacg cctgcggcat caacatccag ccgtggcatc agatcctgag cgataccgtg
    51061 accacgctgc tgcaagccgg agtagctggt gacacgccag ttatcgccgg gcaatcgttg
    51121 tagcgtcctc gcattcagtt ctgcagttaa agcatcatta acctgccagg gctggttatc
    51181 accagtttgt gccgtttgcc aggcaatatc atcatcgcat aacgcttcaa tacaggtgcg
    51241 aagccctgcc gcatcttgcg gttccccttt ttgcagcaaa cgcccgaggg cactttggtg
    51301 gacgtcggtg tcaccttttt tatcgccacg acggcgcacc agcggtgcaa cgccgagact
    51361 gcaatgccaa accgaacgcg tcagcgccac gtaaagcaaa cgcagatctt ccgccagacg
    51421 ttcgacctcc gcaagatcga cgctttctgg cgcagcgtta agatccagaa cagcctcaaa
    51481 cgagtggcga tcgtgataaa acgcctgatc ctggacgcgg aaattggtaa taaatggcaa
    51541 ccagaccagc ggatattcca gcccttttga tttatggatc gtgacaatct gtaccagatg
    51601 tttatcactt tcgaggcgca tttgttggct ggaggcatta ctgtctggct cgaggatatg
    51661 ttgcgataac cagcgtacca gcgcatgttc actttccagc tgcgttccgg cttcttgtag
    51721 cagttcgctg atatgcaaga tatcggtaag acgccgctca ccgcctgccg ttgccagcaa
    51781 gttttctgca atgttacgcg ccgacatcag cgcccgcatc atcggcataa cgccacgttt
    51841 gcgccagatt tgccgataac catcgaactc ttcgaccacc gcgtcccacg catgttcgtc
    51901 attattcagc gtttcgatat ccagcgcgtt cagccccatc attgacgttg ccagcgcact
    51961 acgcagggtg ttctcacgtt cgggcgtcat caccgcctgc aacaaccaaa gcatttcctg
    52021 cgcttccaga gtttcaaaaa cactgtcgcg gttcgaaagg taaacggaag ggatttccag
    52081 caacgttaag gcatcgcgca cctgggcggc ctcctggcgg ctgcgcacca gcacactgat
    52141 gtccgaagca cgcaccggac gcgcgtcgtc gccgttcatc agcaacgctt cgccccgctg
    52201 tccggcctgt agccagtcgc gaatttgcgc agcacttacc tgcgccatgg tactttgata
    52261 gtcgccaacg ccgcaacttt cgccttccat cagccacatt ttcatcgcag gctgcgtttc
    52321 acctttaaag acaaaacgta acgcctgatt tttcccggct gatttcactg gaataaacgg
    52381 tatttcgcga aacatgaacg cgtcatcagt ctggctgaaa agcttgttca cactgttcac
    52441 cattccaggt gcggagcgcc agttggtgtc taaagtgtag tgggcgtgaa cttcactacg
    52501 cgccttcata taagtgaaga tatccgcacc ccggaatgca tatatggcct gcttcgggtc
    52561 gccaatcagc aacaatgcgg tttccggctg atggtgccag atacggcgaa aaattcggta
    52621 ctgctggggg tcggtatcct gaaattcatc gatcattgcc accgggaatc gcgtacggat
    52681 cgccgctgcc aacacctcac cgctttcgct acgcagcgcg gaatcgagcc gacttaacat
    52741 gtcatcaaaa cccaattcgc cacggcggcg tttttcacgc gctactgttt cgcggatctc
    52801 agccaatgcg cgggtgatca ccagatcgcg gatcgacaat ggttctgcaa gcagttgatc
    52861 gatcgcctca aacagtggat gtcgcggggt ttcccccccg gccttcgtgc gatcttctaa
    52921 gaaacgctgg gagaattttt ccagcgactc cggcaactga taactgtttc tctcttcttc
    52981 tgcccaggcg ctgatcttgt cgatccattt agcctgattg ctacggttaa acttgcgtcg
    53041 atcaatacca gaagattcga tcagcgcatc cagttcaccc actgcgtcgc gccactgctg
    53101 ttttaccgta tcaatacgcg ccacaatttg cgcgtgacgg gaagccagcg tttcatcatc
    53161 gggcggcggt gctttgataa ccggcgcttc accttgcaga taacgattaa tatcgcgcag
    53221 caacgcctgc ggccctttcc aggtttcaaa gacgacctgg gctatttcac gcggcagcgg
    53281 gtagcagtgg cgacgccaga aatcggcgca ggcctggtaa cgtagcagag actcatcttc
    53341 aatcagctgc tgctcaaaca gcatgccgga ttcaaaggca ttcaggttga gcatgcgctg
    53401 gcaaaaaccg tgaatagtaa agactgccgc ttcatccatc tgccgttcgg ctaacaacaa
    53461 ccacttcgcg gcttgcgctt tatcgtcgat ctcttccagc agacgttcgt acagtggatt
    53521 gtcggtggtt tcacgcagac aggcgatgcg caactcatgg atattgctac ggatacgacc
    53581 acgcaattct gccgtggcag cctcggtaaa ggtgaccacc agcagttctt caacggtcag
    53641 cgggcgggga aaggcggcgg aaccgcctag tccaagtaac aggcgcaaat agagcgccgc
    53701 aatcgtaaag gttttgcctg tgccggcaga ggcttcaatc aggcgctcgc cctgaaaggg
    53761 caagcgcaaa ggatctagtg tctcggcgac atcactcatt cttttcactc atcaggggca
    53821 ttgtttgctg caacgcgctg acgttctccc acactttcca gccttcagga tgcacatatt
    53881 cggctttccc gttctggctg ccggaaatct gcgacagaat agccatgcct tgcggctcga
    53941 ccaccgcctg atggaagaaa tcagcaagtt tttgcggcgt cagcagtttt atctgggcca
    54001 cgattttatc acgcgaatcg aagcgcatat tgccgcgatc gaaatcttta cttaacttca
    54061 atgcttcttc gccgagcgtt tgcggtgcct gcagcatctg ggtaattacc gcctgctgga
    54121 tttgcgcaaa ctcatctggc ttcatcgctc gcaattttgc ctctgcggtt gggaaaaacg
    54181 ccttgtaacg ctcccacaag aatgaaggct gtttatcatt gctttgcaaa aggaagccca
    54241 tgccccactg acgccccacg ctcattggaa acgcaaacac ggcatagccc aattgttctt
    54301 cggtacgcaa ctgattgtag aaccacggct gtacgatctg ccccaacaga gagctatagg
    54361 ctgagcttgt gtattcatcg tagccagtcg gtacaaatac cgctgccagt gcggagtcgg
    54421 tgctgttacc ggctttttca aagatgacgg attgtttttt atcgaccact acatctttgt
    54481 ttcgacacca ctctgaacca tcagcgccca actgtttttg cacatcgcgt gccagcgttg
    54541 ttgcctgggc ctcggtcatg ttgccgataa ccataaactc tggtcgagcc cctgatttta
    54601 aggcgtcacg ataagccagc acctctttca acgtaatgga gggcaaaatt ttacgccgtt
    54661 catctcgcga gaagtacggc acttgcgaga gcatctgcgc gggcataatc gcctgctcaa
    54721 acgctttacc cttttctgcg gaatccatca tctggttata ccaggacttc gcctgctcaa
    54781 gctgatcttc cgtagcggta tagctaaagt atccctcgag caatgcctgg aacagctgcg
    54841 gcagacgctg ggtgtaacca ttagcattaa ccataaggcc gttgttagcg ttggtggaaa
    54901 aacttatgcc accaaccgac gcctggttgc ttaactgatc aagcgccagc cctgcgagat
    54961 aatcattgag cgcaaacatc acctgattgc gggcgctgtc catggctttc ggattacgca
    55021 aaatcaggct gacatcagct ttgggttcgc tggaaaaata acggcttggc gcatacacca
    55081 cgcgcagatt cgactcatca acaatcagct ctggatggtc atatttcttc tctgacttaa
    55141 tcagcgagaa atcgtcagga atataggggt taagctccgg taaggagagc gcaatattgg
    55201 cagctttttg ctgccagtca gcgaaagttt gttcgctgat tttatcgacc tgatacggcg
    55261 catcgacaaa ataagccgtt ttattgtgcg gctctttcgg gctgatatac cagatacgcg
    55321 cattctgcgg cgtcatcatc gccagacgtt cttttactgc tttagcatcg taccgatcgg
    55381 caatattgac tgcatccagc gtatgctcaa caggaacgcg aatcatggta tctgccagcc
    55441 attcgacgta atccatatca cgggtgattg acggataacg gaagtcgata tccagcacat
    55501 tcgccagttc atcgaagtat tgtttatcga tccctttttc acgtaacaaa ttgaggtagc
    55561 taaaaatagc cgccacaacc tgatcgcgat tcgccagacc tttatcggtt aaagacgcag
    55621 agatcgctaa tacgccgctg ttgccgttga cgataggatc ggagttggcg ctaatgccct
    55681 caactaaccc ctgcttttgc agccagtcag acagtgtacc tgggctgcga ttaccaatca
    55741 gataggtaat caattcatcc gttttactgc ggaacttcgc tgagttgtta tcgatgcgaa
    55801 actcaacgcg cagaacttta cgcggcagcg ctgggacgta atgaatgata atgccctttt
    55861 gcgcgtcggt gactaccggc acggtgattt ccggtttttt gctctctttg ttcggcacgc
    55921 gaccaaaagt gtccgccgcc atttttgcca actccggcag cggtttatta ctgtaaataa
    55981 ccgccttcat caaattggcg gaatagtact tctcgtggaa atctttcagc gcctgctgca
    56041 ccggattacc aggtttgtcg cttaaagttt cgaggttacc accagaaaac tttgaaccgg
    56101 ggtgtgccgg gttaatggtt tctgcgctga cctgtgccat gcgcatcccg tcacgtgtac
    56161 gcgccatggt taattcagcg ttcactgcat tacgctcacg ttcggcgtat ttcttgtcga
    56221 gcaagggttc tgcaatagca tcggccaggc ggtctaccgc accgggcaag gcgtcgttct
    56281 caacttccag atagaaagcc gtgcgatacg gcgcagtgct ggcattgtga ctaccgccgt
    56341 gcattttgag atattcggcc agactgtcag cctgcgggta ctttttcgac cccatcagac
    56401 tcatatgttc aaggtaatgt gccagcccct ggtacgcctc gggatcttcc agcgacccaa
    56461 cgggcaccac cagcgccgat agcgatttaa ctgcctgcgg atcagaaacc agcaagacca
    56521 ccataccgtt atccagacgt atagcctgat actggcggtt atctttatca cttttacgga
    56581 tggtttcctg aatcggctgc catcccgttt ctgcctgact taagggtgcc caaagggcaa
    56641 ctaacaacaa taatgctttg aaccaggtgc tgcggggcat tcacggacct cataatcaac
    56701 tttattttct ggccagattc aacaggttaa cgcaaatcat ctgccagaat ttaatcttgt
    56761 gctgcacgag tcagcctatg tttatataac catcagtccg tgactggtgc gcatcataaa
    56821 gtaagcggat agattgcgca atttttatac agcgctcatg actgattaaa gcgaaacagc
    56881 ggtaacagga aacgttgcga ctgttcaacg attgcctcca ttgtctctgg tgttaattgc
    56941 cgccagagcc tttgatacca gatatcatca ccttcgccac tcaccatcat gttgccttcg
    57001 taagcctgaa ggaatttcgt acgggctttt tgcaacgtgg aatcgtcatc cagcatggca
    57061 tcgttttgcg cgtcataaca ggtttttagc cacgcgccgc cactttcagg taacaccagc
    57121 aatggcgcgg acatcccttc acgatacccc tcaatcagtt gtgagaggta atgcaaagcc
    57181 tgttcggctg caagcggcgg aaaacgccac tcgccgtctt tgcgtagaaa aaggcgactt
    57241 tcaccattac caccgctggc acagtagaca aggtgttcca gccaaagttg cattccctgc
    57301 gccacactta ataaagaggg acgccagcgc aacaagccat ccggctgcac ctgcggcaac
    57361 cagccagtta tctgcacacc gttgcaagcg agatcaattt ccatactctg ccccggctgg
    57421 cgacaggcaa tgactctgtc ggcaagctgc tgcatctcct ggcactgtgt ttcccagaaa
    57481 atttcaccaa aagcgccata cggtaaatcc cctgccgccc ggaagcggcg gaacaagcgt
    57541 tcggcatcat cctgctcaac cagtgcattc aataactgct gattgatttg ataacggcta
    57601 agtccttcca gaataaatgg ctcggtgtcg gggatttcgc tgtcttcagt acggaagttc
    57661 acctgcaaac gcatctggaa gaacgcccgc accggatgtg cccagaatcg ttgtagcgtt
    57721 tccagcggca cggtttccgg taaggtaaac ggcagcggct gaacaaattc agaatgtgct
    57781 ttaccagcct ggctggccgc aggtagccat tcacgagcat agctttgtcg ttcgcctggc
    57841 tggtagtttt gcggatcaaa cggcatccgg gtatggaggc aagtaagatg cgcttttacc
    57901 cttgcctcgc tttcatcaca gttgagcgct tcatcgcccg gtagataatg actttgcccg
    57961 atgtagtcga tcagttcctg caccagtacc gacgggaaac gttcactgtt atcctgaatg
    58021 gaacgcccga tatagctgat atagagtttt tgctgcgcgg aaattaacgc ttccaggaac
    58081 aggtagcggt cgtcatcgcg acggctacgg tcgccacgct tcggtttctg gctcatcagg
    58141 tcaaagccca atggcgcaag ctgacgtgga taaacgccgt cgttcattcc cagcaggcaa
    58201 accactttga acggaattga acgcattggc atcagagtac aaatgttaac cggcccggcg
    58261 agaaaacgct ggctgatacg ttcttgatcc aggcgctgtg ccagttcatc acgcaatagt
    58321 gacagcggca ccgcatcgcc atactgcgca cctaaacctt cggcgataat cgcctgccat
    58381 tgttgttcga tcagcgtcat cgccgcttcg gtttccgcat ccggcaggaa gagggcgttg
    58441 agcatatcgc gacaaaccgg caaccactct tccagcggac gctcctgtgc cagcccgcga
    58501 cgccagatgt ttagctgcat tagcagtgaa gccagatgcc ccaccagttc tgcaattaag
    58561 ccgctcgatt catcataagg tagaaccgat tgccactcgc cctgcgcgct ctccatcgcg
    58621 tagcccaaca acatacgcgt caggccaaat cgccaggtgt gttgtccggt ggcggggagt
    58681 tccagctcgc gaacgttgtc gtcatctatc ccccaacgaa tgccggattc gttgacccac
    58741 tgacgtaaat aacgcagccc ttcttcggtg atgtcaaacc gcgccgccag caccggcaca
    58801 tccagtaatg ccagcacgtc ttccgacaca aagcggctgt ctggcaacga taacaggctg
    58861 ataaacgctt caagtacagg atgcgactgc cgcgcccgac ggtcggaaat ggcgtaaggc
    58921 aggtaacgat ccgcaggtgc gctaccaaac acagcctgaa taaacggact gtagctgtcg
    58981 atatcagcca ccatcacgat gatgtcgcgc ggagtaagtg tcgggtcttc ttccagcatc
    59041 gccagcaggc gatcgtgtaa aacttcaact tcacgctgcg ggctatggca aacgtggaag
    59101 gtgatactgc tatccagtgg atcaagcgga cgtttgttat cgctacggga aaactcttcg
    59161 atgttcacac cagcaacggc gcggttttcc agttccagaa tgtcagactg aatgttatgc
    59221 agcaggttat ctggcgtcac atcgacaaag gcgtccagct cctggctgct ctccaggtca
    59281 gaaaggagat aaatgtagtc gcgcccaagc ttgccccatg aggccagcag cgggttgccg
    59341 acatcctgtt caccatcgct gttaaagagc tgcccggcat tttcgctgtc gcgaaatagc
    59401 ggtaattcgc gatcttcaaa actgtgtcgg cgctggcgag tcagtagttt cgccagataa
    59461 gcaggatctt taatgtcgcc ccagtaataa cggcaggggt tggtaaacag gagatggatt
    59521 tcaatatgtt tacccagcgc ctggagcgcc tggagataaa caggcggtaa cgcggaaata
    59581 ccgcatataa agacgcgcga aggtaacccc ggcgggcagg tcgtcgcgga ctccagcgtt
    59641 tcgataaagc gctgatagag attggcgcgg tgccagcgcg gttgtccgag ttcatgggta
    59701 tattccacca gcgccttcca caacggcgct tgccaggcct gtgcctctcc cagcccttca
    59761 accaaatgtc ctgtttccca ctgtgccagc cagtccggac gatagaccag atactggtca
    59821 aacaggtccg ccgcttttga ggaaagctgg aacagctttc gcttgtcgct atcgtcagtc
    59881 agataatgcc gcaacagggt aaagtcttcg cgctccagca attgcggcag cagagtcatc
    59941 agtttccagc tcatgctctg tttgttaaag gcgctctctt tggggatctc cggtaacacc
    60001 cggacgaaca tatcccagat aaagctcgca ggcagcggaa aatcaatgtt tgccgcaata
    60061 ccaaactttt gcgacagggt catttgcagc cactgtgcca taccggtact ttgcaccaga
    60121 atcatctctg gttcgaaagg atcgtccagc cgttcgcgtt cgacaataaa ctccatcaac
    60181 gcttccagca cgtccagacg attggaatgg tagaccctta acatagcggc tcctgactac
    60241 tgacgattcg ggcaatgcag gcgggtcatc tcgcctgctc tgcccccggg tgaaactagc
    60301 gtaacgctga tgctgacaca tcccgcctgc gatgtctgca ttcggttgac ctgccagttg
    60361 gcaggtggcg aaatcgcgcg caattgcgtt tgctgccaac catgccgcca gagctgttgg
    60421 tactggtttc tgctggtaag actgttcatt aatgttcgct ggaaacccga taacgcagtg
    60481 acaatcatca ccatcaacac catcgccaac attacctccg gcaggctaaa gccttgttga
    60541 ttcttcaggg aagctgacat aacgccccct ctttcagcgg acaaaaatcg ctccagccgc
    60601 gtggcgaaaa aacaattttt ccatcaatga cttcgcctgt tcgccacaac gaaacgcctt
    60661 cataaccggc aattaataag gcttcattat cttccagtaa acgcaaacaa acccgggcac
    60721 cagtggcagc gtacagtaag cactgaactg cgggctgcgt ctgccaggag tgcattttcc
    60781 cccacgccaa cgccgactga acgatggcct ggcggcgcag tgactgactt tccatgctca
    60841 cgcgagaggc aaaactgcga tcctgctggc tcattccttg taatagcaag ctacccaaaa
    60901 ccagcagcat caggaccaga gccagtgacg aaacaccctt ttcgcggttc acaggttgaa
    60961 tcctgtcacg ctatagctgg catccaccac ggtttgcggt tcagacttac tggcagcacg
    61021 catattaacc gtcaacaccg gcgagaagcc gctgacatcc tgacgtacga cctgaaaagt
    61081 gtcgataatg atggcatccg gattagtgac tttatcccag cccttacctt cacaggatgt
    61141 cgcaccgcgt agcgtttcca acacttgctc cttcagacga aatccaatct ggtcggactc
    61201 ttttaccggt tcgcgatccc agataccgtt actgttcgca tcccactgca caatgataca
    61261 gtcaccctgt ccgacaattt ccagcccttc gccggtacag atgccatgac aataacccgc
    61321 cctctggaga tgcttcgcga cggtaaatac ccgcagccag atttcatctt ccagtgccag
    61381 cttacgggtg ctcgttaaac tttcacgctg taacgcaggc agaaagcgtg cagcccccaa
    61441 caacaatacg ctactgatcg ccatagcaat caacacttcc agcagagaaa aaccttgctc
    61501 ttttacaggc atccttctgt ttctccttgc tgacaaagcc ggagtcttcc ccacggcgaa
    61561 accaccagcc accactcgcc cgttgagttt ttgaagccaa tatgcccggc ccatgcggta
    61621 ttgcgcaggc caaagaaagc aagcgaaggt gtcaggtcgc tcatttcgac ttcgggccag
    61681 cgtggcacaa agaccaatgg tgaactgcca tgacaggtat tcgccccagc aacggaactc
    61741 acaaggcacc ataacgtccc ctccctgata aaactgatac tgtggtcgcg gttatgccag
    61801 ttggcatctt cacgtaaata gagcaaatag tcccgcgcct ggctggcggt ttgccaaagc
    61861 cgttgcgact gctgccagta ttgccagcca tagagtccac ttgcgcttag catgaccaaa
    61921 atcagcatcg cgaccagcgt ttcaatcagc gtataaccac gttgtgtttt catgccggca
    61981 gtatggagcg aggagaaaaa aagacgaggg ccagtttcta tttcttcggc gcatcttccg
    62041 gactatttac gccgttgcag gacgttgcaa aatttcggga aggcgtctcg aagaatttaa
    62101 cggagggtaa aaaaaccgac gcacactggc gtcggctctg gcaggatgtt tcgtaattag
    62161 atagccaccg gcgctttaat gcccggatgt ggatcgtagc cttcaatctc aaagtcttcg
    62221 aaacggtagt cgaagatgga ttcgggttta cgtttgataa tcaacttcgg cagcggacgc
    62281 ggttcacggc ttaattgcag atgagtttga tccatatgat tactgtacag gtgcgtatcg
    62341 ccaccggtcc agacaaaatc acccacttcc agatcgcact gctgcgccat catatgcacc
    62401 agtaacgcgt agctggcaat gttgaacggc aggccgagga agacgtcaca ggagcgctga
    62461 taaagctggc aagagagttt gccgtctgcc acatagaact ggaagaatgc atggcacggt
    62521 gccagcgcca ttttatccag ttcgcctacg ttccacgctg aaacaataat gcggcgggaa
    62581 tccgggtcgt ttttcagctg gttcagtacc gtagtgatct ggtcaatatg acgaccatct
    62641 ggcgttggcc aggcgcgcca ctgtttacca tacactggtc cgaggtcgcc gttttcatcg
    62701 gcccattcgt cccagatggt gacattgttt tcgtgtagat aagcaatgtt agtgtcgccc
    62761 tgcagaaacc acagcagttc atggatgatg gaacgcaggt ggcaacgttt agttgtcacc
    62821 agcgggaatc catcctgcag gttaaaacgc atctgatgac caaaaatgga aagcgttccg
    62881 gttccggtac ggtcgttttt ctgtgtgcct tcgtcgagca ctttttgcat cagttctaaa
    62941 tactgtttca tggttcctca ggaaacgtgt tgctgtgggc tgcgacgata tgcccagacc
    63001 atcatgatca cacccgcgac aatcatcggg atggaaagaa tttgccccat gctgatgtac
    63061 tgcacccagg caccggtaaa ctgcgcgtcg ggctggcgga aaaactcaac aatgatgcga
    63121 aacgcgccgt agccaatcag gaacaagcca gacacggctc ccatcgggcg cggtttacga
    63181 atatacaggt tgaggataat aaacagcacc acaccttcca gcagcagctc gtaaagctgt
    63241 gatgggtggc gcggcagcac accgtaagtg tcgaaaatgg attgccactg cgggttggtt
    63301 tgcagcagca aaatatcttc tgtacgggag cctgggaaca gcatggcaaa cgggaagttc
    63361 gggtcaacgc ggccccacaa ttcaccgtta ataaagttgc ccaaacgccc ggcaccaaga
    63421 ccaaacggaa tgagtggtgc gataaaatca gaaacctgga agaaggaacg tttagtacgg
    63481 cgggcaaaga taatcatcac cacgataacg ccaatcaggc cgccgtggaa agacatgccg
    63541 ccgtcccaga cacggaacag atacagcgga tcggccataa actgcgggaa attgtagaac
    63601 agaacataac caatacgtcc cccgaggaag acgccgagga agcccgcata gagtaagttt
    63661 tcaacttcat ttttggtcca gccgctgccc ggacgattcg cccgtcgtgt tgccagccac
    63721 attgcaaaaa tgaaacccac cagatacatc aggccgtacc agtgaagcgc cacgggtcct
    63781 attgagaaaa tgaccggatc aaactccgga aaatgcagat agctactggt catctgtcac
    63841 cacaagttct tgttatttcg ctgaaagaga acagcgattg aaatgcgcgc cgcaggtttc
    63901 aggcgctcca aaggtgcgaa taatagcaca aggggacctg gctggttgcc ggataccgtt
    63961 aaaagatatg tatatgatcc gcgctataac cctccgcgaa tcagcccgcc catgccgcga
    64021 cgctccataa aggctgcaac ctgatggcga acttcggtcg ccagttgcgc ttccagacta
    64081 cgctgcgcaa gattttctgc ttcggcataa tcaatgcgcc gcagcaggta ttttacccgc
    64141 gctacagaac gtccgttcat agacagatgg cgatacccaa gcccaatgag gattgccacg
    64201 cacatgggat cgcccgccat ttcaccgcac aaacggagat cgattccatg tatttccgct
    64261 tcccgggcga tcatcgccag agctcgtaac attgcaggat gaagactgtc ataaatgttc
    64321 gccacccggg tattgttgcg atcaacggcc agaatgtatt gagtcagatc gttggtgcca
    64381 acagagatga aatcgacccg ctttgccaga tgcggcagca taaataccat tgacggcact
    64441 tccagcatga tgccgatacg tggtttggga atttcgtaac cgatcatctc ctcgacttca
    64501 cgtccggcac gttcaatcag gcggcgtgct tcgtcaactt catcgaggct tgtgaccatc
    64561 ggcaacagaa tattcaggtt gcccgtagcg gcattagcac gcagcatcgc ccgcacctgg
    64621 atcaagaaga tctccggctg atcgagcgta atgcgaatcc cacgccaacc caggcatgga
    64681 ttctcttcgc taatcggcat gtaaggcagc tgcttatctg ctccgacatc cagcgtacgc
    64741 aaggtgacgg gtttatcatt gaacatttgc agcatcccct gatactgcgc cacctgttct
    64801 tcttccgacg gaaaaccact ttgcagcatg aatgggattt cagtgcgata aagaccgatg
    64861 ccatcaatac ggctgcccag tttttcttca tgttccgggc ttaaaccagc attgagcatg
    64921 acttttatac gctcaccgct ttttaactgc gcgggtaaat tgacgtcatc ttccgccaga
    64981 cggctaagct caatctcttc actaattagc cgctgatatt cttgcagcag taccggctcc
    65041 ggatcgacca gcaattcacc gcgatagcca tcgacgatca gcgtccgacg atgcagcacc
    65101 gaaggctgaa tatccgcgcc catcacggta gggatcccca gcgcacgtac catgatcgca
    65161 gcatgggagt tggctgcgcc atctcgcacg acaacaccga ctaagcgatc ctggggcagc
    65221 tcagcaagag ttgtcgctga cagttcatct gccaccagaa tgaaacgttc cggccaggcg
    65281 ttcggccctt gattagcgtc atcaagatga aacagcaatc gctgacccag cgcacgtaaa
    65341 tcgccagccc gctctttgag atagttatcg cttagcgcgg caaactgttc ggcaaatttt
    65401 tcaatgaccg tttttaccgc ccattctgcc accgagcctt tatcaacctc ggcaaacaat
    65461 tcgcgacgca gccgggtgtc cgaaagcagg tgcgagtaaa gatcgaaaat agccgccgtt
    65521 tctttttgtg caccggcggc aaagcgtttg ctgtagcggc gaaactcgtt tgccgcttct
    65581 tccagcgccc cggtcagtcg ttcgcgttcc agagccggat ccagcgttga tgcctgatac
    65641 acctgttcca ttaaaggtaa cgtggcatcc tgccagcctt cggcaatcgc tacaccaggt
    65701 gctgccggta atgcgcggat tcgcgtctgg cgatattgcc caaacaaggc agtcaactgc
    65761 gactgagaaa gaatcgctgc catctgggtg gcaagcgtca ccaggaagga ttcttcactt
    65821 tcgtcatact ggcgcaactc tcgttgctgt accaccagta caccaagcaa ctggcgacgt
    65881 tgaataattg gtacgcctaa aaacgcgcgg aaacgttctt cttttacgga ggggatgtat
    65941 ttgaagctgg ggtgcttttg cgcatctgca aggtttatcg gttccgccag cctgccaacc
    66001 aggccgacga tcccttcatc aaaggcgagc gttacagtgc gaccgcgtgg ttttttcagc
    66061 ccccgggtcg ccatcaggta gtaacaacgt cgatcatgat cggccaggta gaccgaacag
    66121 acctcggtat ccatcgcaag acagatgtcg gtaaccagaa tatttaacgc ctcattcagg
    66181 cgtggtgcgc tggctacctt ttcgactatt tcgcgcaggc gagtgagcat aattggcgtg
    66241 acttaacctc ttttacgtcg ataagcagat gcgttttgtg gttttggcgt attttcctgc
    66301 agtgacatca ccacactcgc gaactctttc attaccctac ggtagacatc acgtttaaat
    66361 gacaccacct gtctgaccgg ataccagtaa cttacccatc gccagccgtc aaactctggt
    66421 gtactgctgg tttgcatatt gatttctgca tcgccgctca ccagctgcaa gagaaaccat
    66481 ttttgttttt ggccgataca aaccggcttc gtgtcccaac gcaccaaacg tttcggtaat
    66541 ttgtagcgca accagttacg cgttgaagca aggattcgaa cgtctttgcg gcttaatcct
    66601 acttcttcaa acaattcacg gtacatcgcc tgctctgcgg attctccggg gttgattccg
    66661 ccttgcggaa attgccagga gtgctgacca aatcgccggg cccacattac ctgcccctgg
    66721 cgattacaaa tcacgatacc tacgtttggg cggtagccat cgtcatcaat caccggacta
    66781 cctcaaaata aagctttata tacgaatgat tgtttcatac tccaggaaga cggtaaacca
    66841 ctctctgcag ggcattacac actaacaaca attgaataac tcacagttat gtgcagagtt
    66901 ataaacagag gaaggggtgg atagccgttt ttattcacct tttctgtgga tagagttgtg
    66961 aagaactacg gaattactac gggaaaaccc ggagcattct gaataagccc gatatagaca
    67021 ttttaaaata ttcattatta gtcaatgcat tacatgtaat ttaagtaatg aaaaagagta
    67081 attcgtgacc caggtcacac ctctcattta cgggttggcg aaagatcaac caatgccgta
    67141 tttatccaca gaatgtgcca ctaagttaag cactgaacca ctaaaaactg gagtttcgtc
    67201 gcacgtcaag gctgtaaatg gaaacagtag tggaggtttt tcacagttat cccagctttc
    67261 tgtggataac atggtgtaag atcctgttta ttttcagtga ccagatttgg aaaacccgtt
    67321 gcagtgttgc gcaactcgat taccggcaac ctaaaaagca atataaatca gataattgaa
    67381 caacgcatgt ggaaaaagtt acactgcgaa tattcggcac ataattgctg tttgtttttt
    67441 aaccaaggta tcatgacatg tcccaacctc gcccactgct ctctcctccc gaaactgaag
    67501 aacagttgtt agcgcaagca cagcaacttt ctggttatac attgggagaa ctggcggcac
    67561 ttgccgggct ggttacgcca gagaatttaa aacgcgataa gggctggatt ggcgtgttac
    67621 tggagatctg gctaggtgcc agcgcaggga gtaaacctga acaagatttt gctgctctgg
    67681 gcgtggaact taaaactatc cctgtggata gtcttggtcg tccgctggaa acaacattcg
    67741 tttgtgttgc cccgttaacg ggcaatagcg gagtgacctg ggaaaccagc cacgtgcgcc
    67801 acaaactcaa acgcgtactg tggataccgg ttgaaggcga gcgcagcatc ccgctggcgc
    67861 agcgtcgcgt tggatcaccg ttgctgtgga gcccgaatga agaggaagac cggcagctac
    67921 gcgaagactg ggaagaatta atggatatga ttgttctcgg tcaggttgag cggattaccg
    67981 ctcgtcacgg ggagtattta cagatacgac cgaaagcagc gaatgcgaaa gcgcttaccg
    68041 aagccattgg tgcacggggc gaacggattc tgacgctgcc gcgcggcttt tatttgaaga
    68101 agaatttcac cagtgcacta ctggcccgtc attttctgat ccagtagcca tcgctttgac
    68161 ctgccgcttt ccgggcatat aattaccgct tcattttttt ggcagggctt tttagatgtt
    68221 atttgcatgg ataaccgatc ctaacgcctg gcttgcgctc ggtacgctga cgctgctgga
    68281 gatcgttctt gggatcgaca atattatttt cctttctctg gtggtggcaa agctccccac
    68341 agcacaacgt gctcatgcgc gccgtctggg gttggcggga gccatggtta tgcgtctggc
    68401 gctgctggca tcaatcgcct gggttacgcg cctgacgaat ccgcttttta caatattcag
    68461 tcaggaaatt tctgcccgtg acttgattct gcttctgggt ggcttgttcc ttatctggaa
    68521 agccagcaag gaaatccacg aatccattga aggtgaagaa gaagggctga aaacacgcgt
    68581 ttcatcattc ctcggcgcta tcgtgcagat tatgctgctg gatattatct tcagcctcga
    68641 ctcggtgatt accgctgtgg gtctgtcaga tcacctgttt attatgatgg ccgccgtggt
    68701 gattgccgta ggcgtgatga tgttcgccgc gcgctcgatt ggtgattttg tcgagcgcca
    68761 tccttcggta aaaatgctgg cgctctcttt cctgattctg gtgggcttta ccctgattct
    68821 ggaaagtttc gacatccacg taccgaaagg ttacatctac ttcgcgatgt tcttctctat
    68881 tgcggttgaa agcctcaacc tgattcgcaa caaaaagaac ccgctctgat actccgtacg
    68941 ctctcctgcg ggagggcgta ctcacttctg ttaccctcac agtttaagat tttcctgctt
    69001 tcaagatata taacgtcagt ttataaacag actattatca taggtgggcc aaatgaggat
    69061 aaccggatga aaaaatgggc agtaataatt tctgcagtcg gactggcgtt tgctgtttcc
    69121 gggtgttcca gtgattacgt catggcgacc aaagatggcc gtatgatttt gaccgatgga
    69181 aaacctgaaa ttgatgatga taccgggctg gtgagttatc acgatcagca aggtaacgcg
    69241 atgcaaatta accgtgatga tgtttcgcaa ataattgaac gttaacaaat aaggtcagca
    69301 tccggctggc cttaagattt ttctctttcc cttttccctt ccctctgcca tttttatatt
    69361 ccttatgtcg tgattataaa aaggaaacgg ctatgcaata tcaccgtata ccccacagtt
    69421 cgctggaagt cagcacgctg gggcttggca cgatgacgtt tggtgaacag aacagcgaag
    69481 ccgacgccca cgcacaactc gactatgccg tcgctcaggg cattaacctt atcgacgttg
    69541 ccgaaatgta cccagtacct ccgcgccccg aaacgcaagg gttaaccgaa acctacgtcg
    69601 gcaactggct ggcgaaacat ggcagccgcg aaaagttaat tatcgcctcc aaagtgagcg
    69661 gaccgtcgcg caataatgac aagggcatcc gcccggatca ggcgctggat cggaagaata
    69721 tccgcgaagc gctgcatgac agcctcaagc gtctgcagac tgattacctc gatctttatc
    69781 aggtgcactg gccgcagcgc ccaaccaact gcttcggcaa actcggttat agctggacag
    69841 attctgcgcc tgcggtttcg ctgctggata cgctggacgc actggcagag taccaacgag
    69901 cgggaaaaat tcgttatatc ggcgtgtcga acgaaactgc atttggcgta atgcgctacc
    69961 tgcatctggc ggacaaacac gatctgccgc gtattgtcac cattcagaac ccttacagtc
    70021 tgttaaaccg cagttttgaa gtaggtctgg cagaagtcag ccagtatgaa ggggtcgaac
    70081 tgctggccta ttcgtgcctg ggtttcggca cgctgaccgg gaaatatctc aatggtgcaa
    70141 aacccgctgg cgcacgtaat acgctcttta gtcggttcac ccgctatagc ggtgagcaaa
    70201 cgcaaaaagc cgtcgcggcg tatgttgata tcgccagacg tcatggcctg gcccctgctc
    70261 agatggcgct cgcgtttgta cgccgtcaac cgtttgttgc cagcactctg ctgggcgcaa
    70321 ccacgatgga tcagctgaaa actaacatcg aaagtttgca tctggagtta agcgaagacg
    70381 tattagctga aattgaagcg gtgcatcagg tttatactta tccggcacca taagattaac
    70441 gccggttaaa accggcgtta aatattaatg acggcgctgc cagatccaca gcgccgttat
    70501 tgccagcgca aatagcgcac cgaagccaat gccaatgggc acgaccggga tgcctaccat
    70561 taccgccagc gagtaaatgc ccagcatcaa caacatggcg ctgttttcgc caaggttttg
    70621 tactgcaatc gcattccccg ccccgacgct ttttttaccc cgctcctgta gcaacgcatt
    70681 gagcggaacg acaaaaaagc cccccaacac gccaatcagc atcaacaagg cataggctgg
    70741 cagctgctcg tgttgcaggg agaaaatcag taccaccacg ccaatcaaaa ttccggctgg
    70801 catacagcgt gacacggttt ccagcgtaac cagcttcgct gccgcacctg cgccaaccac
    70861 gataccaatc gctaccatcg cgttgagata ggtcggcgta gcgttatcgg taatgcccag
    70921 cgccaccggt acccacagca ccaacaggaa acgcagcgtg acacccgctc cccagaataa
    70981 actggtgccc accagcgaaa aacgcgtttc accattgcgc catagcgagg tgcaggcatt
    71041 caggaaactg cgggtcatgt tgatgagatt ccaggactgc cccggacgcg ccgccgccag
    71101 tttgggaatg tagatattgg caacgaccgc accaccgtag gccagtgcgc atgcggccag
    71161 ggcgaccagg acatgccagt cagccagcac accaccggct acggaaccga gcaaaatcgc
    71221 cgctatggta gaagcttcca ttaaaccgtt agcttttact aacttactac ccgtggttaa
    71281 ttcgccgaga ataccgtatt tcgccggtga ataggctgca gcaccaacac ccaccagcgt
    71341 atagccgaga aacggattga taccaaagca gatactggct gcgcccagca gcttcaggcc
    71401 gttggcaaac atcatcaccc ggcctttggc gaagctatcc gccacctgcc cgacaaacgg
    71461 cgcaaaaaga atgtaagcac ctacaaacac catttgcagg atgggctggc tccactccgg
    71521 atagaactgc gctttcagta acgccagagt ggcaaacagt agggcattat cgccaaacgc
    71581 agagagaaac tgcgccacga taaccgcttt catccccttc gaccacaacg aagtgttagt
    71641 gtgcactgac tcactcatcg tgttgttcca cttcgtctac ccagcttttc aacgtgacaa
    71701 agtcaggttt gccgctgcca agtaatggca tctgtttcaa atagcgaata tcacgcggta
    71761 cagcaagctc cggcacgccg tgctcacggg catactgttg caacttatcg cgcgtcagtt
    71821 cgttatctgt ggtgaaaagc accagtgcct cgcctttgct ggcatcgctc ttaatcgcag
    71881 tggcatggac tttatctggc gaaacaccaa gtgccagttg ttccaccatt tccagcgaca
    71941 ccatttcgcc tgcaattttg gcaaagcgtt ttgcgcggcc ctgaatctgc acaaagccct
    72001 gctcgtcaaa acgcacaata tcgccagtgt cataccagcc gcgctccatt tcgccgcgaa
    72061 cattctcggc ggtgggcact tccagtacac ctggcttctc cacccgcaga tagccgttca
    72121 ttatgttcgg ccctttcagt tgcaggcgtc cgccctcttc gataccaggg accgacaaca
    72181 ggcgcgcatc cattcctggt agaatacgcc ctaccgtacc gggtttcgcc gccatcggta
    72241 cgttgataga aacgacaggc gcgcattcgg tcacaccgta gccttcaagg atgcgcaggc
    72301 caaatttatc ctgccaaagc tgtttggtac tttcttgtag tttttctgcg cctgccacca
    72361 catagcgtag acgatagaag tcatacgggt tggcgaaacg cgcgtagtga ccgaggaaag
    72421 tcgaagtgcc gaacaacacg gtgcaactgc ggtcatacac caactccggc acaatacggt
    72481 aatgcagcgg gcttggataa agaaacactt ctgcacctgt gagcagtggc gtaaacaggc
    72541 ctaccgtcag cccaaaggag tgaaacagcg gtaacgccga cataaagcga tcgttggtgg
    72601 tgaagtcggc aatcgtttta atctgctcga cattcgccag aatgctttta tggctatgga
    72661 cgacgccttt tggatggcct tcagaaccgg aggtaaaaag gatcagcgcc tcttcttccg
    72721 gctgctgttt aacctgtgcc agacgcggca tcagcaaatg agcgaagatc catactttgt
    72781 cggcagtggt gacatctgct tttaaatctt ccagatagac ccagcgcacc tgagtaagtt
    72841 gctccggcag atgccagagt ttgcctttat cgagaaactg gcgggaagtg aagatggttt
    72901 tgatttcagc cgccgtaata gcactggtca gcccttttac cccagcagtg tagttcatca
    72961 ttgcgggaat gcggcgacgg gcgatggccc caaaaatcac tgccgcactg atgcctgcat
    73021 tgggcagcat taagccgatg cgttcgcctt caacactgta tttttcaagg atgcgtccaa
    73081 caaacagcgt tttcgtaagc aatttgcgat aggagtctgg ggtaaaattg acgtcttcga
    73141 cacatttctt ccctgctccg aagcggtaca ttgcactcag taaagattcg tacagcgttt
    73201 cacgcgggcg caccgccatt cgcgcttcca tcattatttg atgcagcatt tcgccagcga
    73261 ttttgcgacg gtcacgggca cgcggcgcat ccggcatctc cacctgcgtt ggtggcaaaa
    73321 tatgcagagt aatttgcggg aacaagcgac gtttaaccag acctttcagg cggctgaagt
    73381 gcgtaagttc cgccccttca atacgcacag gaataaccgt tgcaccagac ttcgccgcga
    73441 caaaacccgc gccatcgtag attttcatca gcgagcctgt ggtggtgatg cgtccttcag
    73501 ggaaaatcac cactggtcgg ccctgttcca ccagacgtac cagatgttta atcgccatag
    73561 gttgcgtcgg gtcgagagga acaaagtcga taaatgattt cagccaacgc atataccact
    73621 gttggcttat tgaggtgtaa acggcaaaca ctggacgcac aggtaaaaac agtccaagca
    73681 aaatgccatc aataaaagag acgtgattag gcgtaattag aacgcgctcg cccttcagtg
    73741 cctgggggtc acccgtaacg cgaacgcgat acaaaacacg gcacaaattt cgaaaaaagc
    73801 taaaaagcat tccaactccc tttgctctga ttcagtaaaa gcgaatggag ggagattaca
    73861 cgagataaag aacgcgagcg acagtaaatt aggtgcgaaa aaaaacctgc gcatccgcgc
    73921 aggttggtgc aagagacagg gtacgaagag cgtaccgaat aatctcacca atcaatacct
    73981 ctgggatctt gattgtggtc tgcacgacga ctcttcgcca gcgagaaaac gcaaaggaat
    74041 gaagggaaat gcaacgaggt gtgtaaattg tcggttactg ttacagattg atgaccggca
    74101 aaaaaaaacc tgcgcatctg cgcaggctgg tgtaattcat gtgctcaacc cgaagttgac
    74161 ttcacctatc aatacctctg ggatcaccac tttagcaacc tgaagccaaa cgccaccagc
    74221 ggtcaatcgc aacagcgttt cgcaaagtgt aaccaaaggt ttgcaatctc tttttttgtc
    74281 ttacttattt gcgtttggct cacattccca cgatgaaaac acgccgcccc ttgaaccaac
    74341 gggcgttttc cgtaacactg aaacaatgta agcgtttacc cactaaggta ttttcatggc
    74401 gaccataaag gatgtagccc gactggcagg cgtttcagtc gccaccgttt cccgcgtcat
    74461 taataattca cccaaagcca gcgaagcttc ccggctggct gtgcatagtg caatggagtc
    74521 tcttagctat cacccgaacg ccaacgcccg tgcgctggcg cagcagacca ctgaaacggt
    74581 cggtctggtc gttggtgatg tttccgatcc gtttttcggt gcaatggtga aagcggtcga
    74641 acaggtggct tatcacaccg gtaatttctt attgattggc aatggttacc acaacgaaca
    74701 aaaagagcgt caggccattg agcaactgat ccgccatcgc tgtgctgcgt tggtcgtcca
    74761 tgccaaaatg atcccggatg ccgatttagc ctcattattg aaacaaatgc ccggtatggt
    74821 gctgatcaac cgtatcctgc ctggctttga aaaccgttgt attgctctgg acgatcgtta
    74881 cggtgcctgg ctggctacgc gtcatttaat tcagcaaggt catacccgca ttggttatct
    74941 gtgctctaac cactctattt ctgacgccga agatcgtctg caagggtatt acgatgccct
    75001 tgctgaaagt ggtattgcgg ccaatgaccg gctggtgaca tttggcgaac cagacgaaag
    75061 cggcggcgaa caggcaatga ccgagctttt gggacgagga agaaatttca ctgcggtagc
    75121 ctgttataac gattcaatgg cggcgggtgc gatgggcgtt ctcaatgata atggtattga
    75181 tgtaccgggt gagatttcgt taattggctt tgatgatgtg ctggtgtcac gctatgtgcg
    75241 tccgcgcctg accaccgtgc gttacccaat cgtgacgatg gcgacccagg ctgccgaact
    75301 ggctttggcg ctggcggata atcgccctct cccggaaatc actaatgtct ttagtccgac
    75361 gctggtacgt cgtcattcag tgtcaactcc gtcgctggag gcaagtcatc atgcaaccag
    75421 cgactaaccg cagttaaagc aattccagcg ccagtaattc ttcgatggtc tggcgacggc
    75481 gaatcaaccg cgcctgacca ttatcaaaca gaacttctgg taacagcgga cggctattgt
    75541 agttggatga cattgatgcg ccatatgccc ctgtatcatg cagtaccaga taatcacctg
    75601 ccttcacttc cggcaaggcg cggggttcaa catttccccc ttcctgctgg gtaaagacat
    75661 cgcccgattc acataacggt ccggcgacga cggtttccac cgttggtgcg tgttccagag
    75721 aacgaccatc agctgccagg gcactgatat ggtggtaact accgtacatt gccgggcgca
    75781 tcagatcgtt aaacccggca tcaaccagca caaagtggcg gctacccatt tgtttgacgc
    75841 tccgcacctg agtaattaac acgccagact gcgctaccag gaagcgaccc ggttcaattt
    75901 ccagtttcac agggtggccc aaatggcggg cgatttgctc acgcgcggca ttccacagac
    75961 cataataatg ttcggtatca accgcctctt caccctgttg ataaggaata gaaagcccac
    76021 cgcccgcaga aatagcctgt aaatcctgac cgaattcgat gacctgacgc accatagcac
    76081 cacacacctg ttccagatgg gcataatcaa cgccagaacc aatgtgcatg tgaatgccga
    76141 ccagctgcag atgatgacgt tgtatcacgt ccagtgcggc gggcagatcg gtgtaccaga
    76201 taccgtgctt gctgttttcg ccaccggtat tggttttttg gctatgtccg tgaccaaacc
    76261 ccggattaac gcgcagccat acccgatgcc ctggcgaaac ctggcccagt tggtcgaaca
    76321 tatcaacaga acccgcattc accggaattt gcaattcact gacgcgttca agcgtcgcct
    76381 gatcgataac atctgccgta aaaacaatat catcggggtg cgtttgcgga ttgtaacccg
    76441 ccgccaacgc acgctctatt tcgcctaacg agacggaatc cactttcacg ccctgctcac
    76501 gcattaagcg caaaatatga atattggaac aggctttctg tgcaaagcgc accacatcaa
    76561 actgtttcag cgctgcaatc tgccgacgaa taatttgcgc atcgtagacc cacaccgggc
    76621 agccaaattc agcgggcaaa cgcagcagat tttcggcggt gagatcggta tcggtgctga
    76681 acagtgaatg tggcataaca aactccagat gagtgctttt ttatgattac gccacatcat
    76741 aaaaagaata aaaaatatcg atttatgtcg agtctatgca aaaatgatat ggattaccgg
    76801 attgcgagag agcgctaatg gccgccgtta acttacgtca tattgaaatt tttcatgcgg
    76861 taatgaccgc cggaagcctg actgaggcag cacacctgct acacacctca cagccaaccg
    76921 tcagccgcga actggcgcgc tttgagaagg tgatcgggct gaaattgttt gagcgcgtac
    76981 gtggacgatt acatcctacc gtgcaaggac tgcgtctgtt tgaagaagtg caacgatcct
    77041 ggtacggact ggatcgcatt gtcagcgccg cagaaagtct gcgcgagttt cgccagggag
    77101 aactgtctat tgcctgcctg ccggtctttt cgcaatcttt tttaccgcag ctcctgcaac
    77161 cctttctggt acgttatccc gatgtcagct taaatatcgt gccccaggaa tcaccgctac
    77221 ttgaagagtg gctctcggcc cagcgtcatg atttaggact cactgagaca ctccatacgc
    77281 ctgcgggaac agaacgtacc gaattactct ctttagatga attgtgtgtg ttacctccgg
    77341 gtcatccgct ggcggtaaaa aaggtattaa cgccggatga ttttcagggt gagaactaca
    77401 tcagcctttc ccgtactgac agctatcgcc agttgctgga tcagctattt actgaacatc
    77461 aggttaaacg acgcatgatc gtagaaaccc acagcgccgc gtcagtctgc gcaatggtac
    77521 gggcgggggt aggtgtttcg gtggttaacc cgctcaccgc actggattat gcggcaagcg
    77581 gtttagtggt gcggcggttc agtattgcgg ttccgttcac cgtcagcctg atccgccccc
    77641 tgcaccgccc gtcatcagcg ctggtgcagg cgtttagtga gcatttacaa gcggggttac
    77701 cgaaactggt cacttctctt gacgctattt tgtcgtcagc tacgacagca taaaagcgac
    77761 agcatcctcg gcatggatcg ccgcagtatc aaacacaggc aaaacactgc gctcttctgg
    77821 caccagtaaa ccaatttctg tgcagccaaa aatgacgccc tgtgcgccct gttctgcaag
    77881 gcgagcaatc acttgcgcat aataagcgcg tgacgcttcg gtaaattgcc ccagacacag
    77941 ttcttcaaaa ataatctgat taattttcgc ccgttcatcc gcttcaggaa taagacaatt
    78001 gatggaaaat tgttccgtca gccgcccgcg ataaaaatcc tgttccatgg tgtaacgcgt
    78061 acccagcagc gccacacgag tcattccggc cccggtaatt gcacgtccgg tggcatccgc
    78121 aatgtgtaag aaaggcagag tgcaacgtga ctcaatggca tccgccactt tatgcatcgt
    78181 attggtacat aacacaatac cttctgcgcc cgcccgctgt aagccaagcg ccgcctcagc
    78241 cagaatgtcc ccggttttat cccattcccc gcgacgctgg cactcttcta tttcatgaaa
    78301 atcgacgcta tgtagcagca cttgcgcaga gtgaagccca ccaagccgct gtttaatgcc
    78361 ttcatttatc agacgatagt aaggaatggt ggattcccag ctcattcctc ccagcaaacc
    78421 aattgttttc attcctctct cctgatgtgt gttaccgcaa caaggctgaa taaggaacga
    78481 gacaaacgcc tcaacggcca ggtgcccgat ctctattaac gaaaaaaggg ccggatgtac
    78541 agcacatccg gcccgtgaaa tcagacgccg atatttctca acttctcgcc tgccatcagt
    78601 ttgcgttcga tatgttccag cgtgacattt ttggtttccg gaatgagcca gaaagtgatg
    78661 cccacaaacg caatgttcag cgcagtgtag agccagaacg taccggcagc gccaatgcta
    78721 tcaagcagtg tcaggaaggt cgcgccgata atcatattcg acacccagtt tgtcgtcgtc
    78781 gaacaggtaa taccgaaatc gcggcatttc agcggctgaa tttcagagca caggatccac
    78841 accactggcg cggcgctcat cgcataaccg gcaatacaca tcatcgtcat gccaacagag
    78901 agccaggaca agccactgga agccgtaccg ttatcaaact gcatcaggca atagcccagc
    78961 accagagtgc ctaacgccat cacgctgaaa ccaattttca gagccggttt acgacctgct
    79021 ttatctaccg taaagaccgc aatgaaggtc gcgaacataa aggtcagtcc cacgaccaga
    79081 gtcgcaatca tctgttgttc tgtggtcgta aagcccgcca ttttgaagat acgcggcgca
    79141 tagtacatga tgatgttcat accggtaaac tgctgcatcg cctgcaacaa cataccgaga
    79201 aacacagcac gacggacgtt acggttgatc ttaaacagtg cccaaccgcc ttgttttaac
    79261 ttcaggcttt cacgaatttc gttgagttct tctcgcgctt tttccgacgt atcgcgcagc
    79321 atacgcaaca cttcttccgc ctcaatatga cgcccctttt ccgccagcca gcgcgggcta
    79381 tttggcagga agaccaccag aataatcagc aggactgctg gtaaagcaag aacccccaac
    79441 attgcgcgcc agttaccgct ataactgaac gctgtatcgg ataaaaacgc cagcacgatg
    79501 ccgagtgtga ccatcaactg gtacatactg atcatcttac cgcgaacgtt ttcacttgcc
    79561 atttcagaaa gatacagagg agcggtgtaa gacgcgatcc cgacagcaat gcccagcacc
    79621 acacgagcgg cgattaacat ctctacgctg gtcgcaaaag cggaccctat agaaccgagt
    79681 acaaacagga tggcccccgc catcaggctg tatttacgcc ccaggcggaa cgacagccaa
    79741 ccattaaaca gcgcaccaat tgctgcgccg agcatcatgc tactaaccac ccattcctgc
    79801 aaacgactgg tcagcacaaa gtgatcggta atgaacggca acgctccggc gattacgccg
    79861 atatcaagac caaataacaa tcctgcgacc gcagcagcta ccgaaacaaa catattcata
    79921 cgccgcgtat cacgcaaaga acgtggcgtt aaagcagatt ccgtattgat agtaaccatt
    79981 ttttcctgcc agcagagagt aagacatagt gaaaaaatac gtgaacaatt cacacaggtg
    80041 tcaggtcgga aacagcataa atatggatta aattgctgcg acatgccgtt atgtgatgga
    80101 tattccaatt ttaaaattaa gttgaattat tgagattatt attaaccacc taattttaaa
    80161 gcagataaaa ttcataaagt tcattaattg ataattgata tggattattt cataaccatg
    80221 atatggatta tgatgatcta gagatataaa aaaccctgcc atgcggcagg gtcataaaag
    80281 taagaggaat gaattaacgc gccagccaac cgccatccac agcaatggta taaccattta
    80341 cataatctga agcgctggag gcaagaaaca ctaccggccc catcaggtca ctcggcagtc
    80401 cccaacggcc agctggaatg cggtcgagaa tttccgcgct acgttgttca tctgcccgca
    80461 gttgttgggt attgttggtc gccatgtaac ccggagctat cgcattaaca ttaatgttgt
    80521 gtttagccca ttcattcgcc atcaatcgcg tcacgcccat cacgccgctt tttgatgcgg
    80581 tataagaagg cacacggatc ccgccctgga aggagagcat tgacgcgata ttgataatct
    80641 tgccgccatt gccttgcgcg atgaagtgtt tcgccgctgc ctgagacatg aagaatacgc
    80701 tcttgatatt caggttcatg acatcgtccc agtccttttc gctgaactcg agagcatctt
    80761 cgcggcgaat caatccggcg ttattcacca ggatatcaat atgaccaaac tcagctaccg
    80821 cgcgatccag cagtccagga ataccatcaa tctttcgcag atcggcggtc aggcttaaaa
    80881 aacgacgccc cagcgccgtg acctgcttga tggtttcagt cggttcaacg atgttaatgc
    80941 caacaatgtc acagcccgct tgcgccagcc ccaacgccat cccctggccc agcccagtat
    81001 cacaaccagt gacgaccgca actttacctt cgagagaaaa tgcacttaaa atcataacaa
    81061 taccttattc gtttatgccc acaactagcg caaatcttta acggccacat ggtccatatc
    81121 atcaaagacc tggttttcac cgaccatgcc ccagataaag gtataagctt tggttccgac
    81181 gccggaatga atcgaccagc tcggggagat caccgcctgc tcgttatgca tcacaatgtg
    81241 acgcgtttct tgcggctgcc ccatcatgtg gaaaacgcag gcgtcatcat ccatattgaa
    81301 atagaaataa acttccatcc ggcgctcgtg ggtgtgacac ggcatggtgt tccacaagtt
    81361 acccggagcc agctccgtca gccccatact caattggcag gtttccagca catccgggac
    81421 aaaatatttg ttaatcgtgc gacggttact ggtgaggtta tcgcctaacg tgactggaga
    81481 tacttcgtcc ggtgtgactt ttttggtagg atacgtcgta tgcgcgggtg cgcaattgta
    81541 ataaaacttc gccggagtgc cggtatcaat actagcaaag acaacttctt ttgcgccttt
    81601 acccacatac agggcgtcgc ggtgaccgat ttcatagcat tgaccatcga cagtaatcgt
    81661 accggcaccg ccaatattga taacacctaa ctcgcgacgt tcaaggaaat aacttacgcc
    81721 gagttgttta cccacttccc cgccaacgga aaccgtttta gttaccggca taatgccgcc
    81781 aataataata cggtcaatgt ggctgtaaac catggtgtac tcatcggcga caaatacctt
    81841 ttcaaccaaa aattcattgc gcagcccctg ggtttccagc gtttttgcgt gcgcactgtg
    81901 gatgctctgt cttacgtcca catcaacctc cgataatcag tgaagcgtga aaatagaaaa
    81961 cgatagtgaa acactgtttc gttttctcta taaggggatg atatgcccat gggatcgggt
    82021 tttcaattaa aaataaaaca atgtttcatt tttaagttaa ggattaaaaa aagtgcagtg
    82081 tcgatcacga atctgtcatg agaatggaaa aatcagggag cccggaaaat gtatgtcgac
    82141 ttgccagcca tcattattca acagattaat gacggcagat atttatcggt aaggaggaaa
    82201 tgcagagcta ttcgtcacgt tcaatggtca atgccacacc ctggcccccg ccgatacaaa
    82261 gcgttgccag tcctttgcgg gcattacgtt tcaccatttc atgaaccaga gaaaccagga
    82321 ttcggcaacc ggaagcgcct atcgggtgac cgagtgcgat cgcgccacca ttgacattga
    82381 cccgacgctc atcccactca agcatcttgc caaccgaaag cgcctgtgca gcaaacgctt
    82441 cattagcctc gataagatca acatcagcca actgccagcc tacacgctcc aggcaacggc
    82501 gggtcgcata caccggcgca attcccatca atgccggatc tacaccaacg ctggcaaatg
    82561 cgcggatgcg ggccagcacg ggtaaattca acgctcgtgc tttggcttcg ctcatcatca
    82621 ttaccgcagc tgcgccatcg tttatggatg atgcattacc cgctgtcaca gaaccgagac
    82681 tatcaaatga aggatttaaa cgggctaaac cttctgcact ggcgtcagtg tgtggctgtt
    82741 catcggtatc aacaaccaac gtctgaccgt tactttgggt cattaccggg acgatctcat
    82801 ctttaaatcg tccggcgtca atcgccgctc gcgctttttg ttgcgagcta agtgcgtaag
    82861 catcctgcaa ttgacggctg atgccatatt cgcgagccag attttcggcg gtgacaccaa
    82921 tatgataatc attgaaggca tcccataacc catcatgcac aagactgtca accagctggc
    82981 tattgccaag ctgtgcaccg gtgcggctat cagtcagaac atgtggtgcg cggctcatgt
    83041 tttcctggcc accggcgatg acaatatcag cctcgccaca ctgtatcgcc tgagtagcca
    83101 gatgcagtgc tttaagcccg gaaccgcaga cgtcattaat cgtgattgca gaaacgctat
    83161 taggcagacc acctttaata gccgattgcc ttgccggatt ctgccctgcc cctgcagtca
    83221 acacctgacc aagaattact tcatccaccg catatgcagg aacgccggta cgttctatta
    83281 acgctttcac gaccagacta ccaagttcca cggcggaatg acccgctaac gcaccgcgaa
    83341 agcagccgat aggtgtccgt aacgccccga caatcacaac gtctttcatc acaacctcgc
    83401 accaattaac agtgcaatag taaatgattg ttaattacag ttatcttaat tgtttaaaaa
    83461 aagtgatttt tatcacaaag gaaatatacc tgagcagtag tcagagtcat aactggcacg
    83521 taaggtttgc aaccactacc ccaccaatag aggggtagat agggcgttaa tctcccatac
    83581 ttaacctggt ttatggtaaa ttgccctcca ttttgtttaa tttgtagatg atacgttcag
    83641 ataatgtcta atatttggtc taaagaagaa actctgtgga gtttcgcgct ctacggcaca
    83701 gccgttggtg caggcacgct cttccttcct attcagttag gttcggcagg ggctgtggtc
    83761 ctgtttatta ctgctctggt cgcctggcct ttaacatact ggccacataa agctttatgc
    83821 cagttcatcc tctcatcgaa aacatcagca ggtgaaggga taacgggcgc ggtaacacac
    83881 tactatggca agaagattgg taatctgatt accacgctgt acttcatcgc cttttttgtc
    83941 gtcgtgttga tatatgcagt ggcaattacc aactcactta cggaacagct ggcaaagcat
    84001 atggttattg atcttcgcat ccgtatgttg gtgagtctgg gtgttgtatt aattctgaat
    84061 ctcatttttc tgatgggacg tcatgccact attcgggtaa tgggattttt ggtattccca
    84121 ttgattgcct atttcttatt tctttccatt taccttgtag gtagttggca acctgatcta
    84181 ttaacaaccc aggtagagtt caatcagaat acccttcacc agatatggat atcgattccc
    84241 gtgatggttt tcgcctttag ccatacgccc attatttcta cgtttgccat agacagacgt
    84301 gaaaaatatg gcgaacacgc tatggataaa tgcaaaaaaa ttatgaaagt cgcttatctc
    84361 atcatctgca taagtgtact gttctttgtc tttagctgcc tgctttctat tccaccttcg
    84421 tatattgaag cggctaaaga agaaggggtc accattttat cggcgctttc tatgctgccg
    84481 aacgccccag catggttgtc aatttccggg attattgtcg cagtagttgc gatgtcgaaa
    84541 tcattcctgg gtacgtactt tggcgttatt gaaggtgcca cggaggtcgt caaaacaaca
    84601 ctacagcagg ttggtgtaaa gaaaagtcgt gcatttaacc gcgcactatc aattatgttg
    84661 gtatcgctga ttaccttcat tgtttgttgc attaacccga acgcgatttc gatgatttat
    84721 gcgatcagcg gcccgctcat tgccatgata cttttcatca tgcctacgct atcaacatat
    84781 ctcatcccgg cgcttaaacc ctggcgctcc atcggaaatc tgattacgct gatcgtgggt
    84841 atcctgtgcg tatcggtaat gttctttagc taatatccat tgtgaatggt tcaaaattgc
    84901 tacaattttg aaccattcca gttttcataa tgtgcatttg ttattattcc gctctatcac
    84961 ttctctcgtc atatcctagt attatcagtt agttaaacac acaaagagat ctgaacatct
    85021 taaataagac tatttaagat gcataactta gattcgcaag atatcttctg gtcattcaaa
    85081 aacaatttgc gatatagata ttctcatacc atgagatagt tatctgaacc accctcaacc
    85141 aggtaagatt aaatcagtga aacataatca t
//
LOCUS       DAZFAB010000008        85158 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZFAB010000008 DAZFAB010000000
VERSION     DAZFAB010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 85158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 85158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..85158
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.008"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..576)
                     /gene="yjgL"
                     /locus_tag="ACHJWA_000660"
     CDS             complement(<1..576)
                     /gene="yjgL"
                     /locus_tag="ACHJWA_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjgL"
                     /protein_id="HFO4307317.1"
                     /translation="MSKISDLNYSQHITLADNFKQKSEVLNTWRVGMNNFARNAGGQD
                     NTRNILNPKTFFEFLVKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSEIMKF
                     VMNNPEGERADSPKVIIEISLSTITTMGTRQGHTAIIFPQPDGSTNRYERKSFERKDE
                     SSLHLITNKVLACYQREANKEIARLLNNHQKL"
     gene            complement(699..1151)
                     /gene="tabA"
                     /locus_tag="ACHJWA_000661"
     CDS             complement(699..1151)
                     /gene="tabA"
                     /locus_tag="ACHJWA_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFO4307318.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            complement(1296..1889)
                     /gene="bdcR"
                     /locus_tag="ACHJWA_000662"
     CDS             complement(1296..1889)
                     /gene="bdcR"
                     /locus_tag="ACHJWA_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO4307319.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLVDILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETAIYDYIARLHPQSAQCVTDFM
                     STVMSGLSAKAREGHSLEQLCATAALAGEAIKTILKE"
     gene            1960..2673
                     /gene="bdcA"
                     /locus_tag="ACHJWA_000663"
     CDS             1960..2673
                     /gene="bdcA"
                     /locus_tag="ACHJWA_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO4307320.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            2804..3199
                     /gene="yjgH"
                     /locus_tag="ACHJWA_000664"
     CDS             2804..3199
                     /gene="yjgH"
                     /locus_tag="ACHJWA_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO4307321.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPTWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            3480..3614
                     /gene="pyrL"
                     /locus_tag="ACHJWA_000665"
     CDS             3480..3614
                     /gene="pyrL"
                     /locus_tag="ACHJWA_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFO4307322.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            3618..4553
                     /gene="pyrB"
                     /locus_tag="ACHJWA_000666"
     CDS             3618..4553
                     /gene="pyrB"
                     /locus_tag="ACHJWA_000666"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFO4307323.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            4566..5027
                     /gene="pyrI"
                     /locus_tag="ACHJWA_000667"
     CDS             4566..5027
                     /gene="pyrI"
                     /locus_tag="ACHJWA_000667"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFO4307324.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            5100..5486
                     /gene="ridA"
                     /locus_tag="ACHJWA_000668"
     CDS             5100..5486
                     /gene="ridA"
                     /locus_tag="ACHJWA_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFO4307325.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(5692..8388)
                     /gene="mgtA"
                     /locus_tag="ACHJWA_000669"
     CDS             complement(5692..8388)
                     /gene="mgtA"
                     /locus_tag="ACHJWA_000669"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFO4307326.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTVVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(8529..8582)
                     /gene="mgtL"
                     /locus_tag="ACHJWA_000670"
     CDS             complement(8529..8582)
                     /gene="mgtL"
                     /locus_tag="ACHJWA_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFO4307327.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            8767..9714
                     /gene="treR"
                     /locus_tag="ACHJWA_000671"
     CDS             8767..9714
                     /gene="treR"
                     /locus_tag="ACHJWA_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFO4307328.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPANLS"
     gene            9833..11254
                     /gene="treB"
                     /locus_tag="ACHJWA_000672"
     CDS             9833..11254
                     /gene="treB"
                     /locus_tag="ACHJWA_000672"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFO4307329.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            11304..12959
                     /gene="treC"
                     /locus_tag="ACHJWA_000673"
     CDS             11304..12959
                     /gene="treC"
                     /locus_tag="ACHJWA_000673"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFO4307330.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPDDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVALNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DESSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMTLRPFEAVWWLQK"
     gene            13353..15491
                     /gene="nrdD"
                     /locus_tag="ACHJWA_000674"
     CDS             13353..15491
                     /gene="nrdD"
                     /locus_tag="ACHJWA_000674"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFO4307331.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDISEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            15681..16145
                     /gene="nrdG"
                     /locus_tag="ACHJWA_000675"
     CDS             15681..16145
                     /gene="nrdG"
                     /locus_tag="ACHJWA_000675"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFO4307332.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(16190..16576)
                     /gene="cybC"
                     /locus_tag="ACHJWA_000676"
     CDS             complement(16190..16576)
                     /gene="cybC"
                     /locus_tag="ACHJWA_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFO4307333.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNSYHKKYR"
     gene            complement(16760..18112)
                     /gene="pmbA"
                     /locus_tag="ACHJWA_000677"
     CDS             complement(16760..18112)
                     /gene="pmbA"
                     /locus_tag="ACHJWA_000677"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFO4307334.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            18206..18757
                     /gene="yjgA"
                     /locus_tag="ACHJWA_000678"
     CDS             18206..18757
                     /gene="yjgA"
                     /locus_tag="ACHJWA_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFO4307335.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(18913..20286)
                     /gene="mpl"
                     /locus_tag="ACHJWA_000679"
     CDS             complement(18913..20286)
                     /gene="mpl"
                     /locus_tag="ACHJWA_000679"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFO4307336.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            20462..21460
                     /gene="fbp"
                     /locus_tag="ACHJWA_000680"
     CDS             20462..21460
                     /gene="fbp"
                     /locus_tag="ACHJWA_000680"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFO4307337.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYSTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFICEFPDA"
     gene            complement(21493..22488)
                     /gene="yjfF"
                     /locus_tag="ACHJWA_000681"
     CDS             complement(21493..22488)
                     /gene="yjfF"
                     /locus_tag="ACHJWA_000681"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFO4307338.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPVYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(22475..23497)
                     /gene="ytfT"
                     /locus_tag="ACHJWA_000682"
     CDS             complement(22475..23497)
                     /gene="ytfT"
                     /locus_tag="ACHJWA_000682"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFO4307339.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIIT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLILFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            complement(23511..25013)
                     /gene="ytfR"
                     /locus_tag="ACHJWA_000683"
     CDS             complement(23511..25013)
                     /gene="ytfR"
                     /locus_tag="ACHJWA_000683"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFO4307340.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFDLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETCELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQNLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            complement(25153..26109)
                     /gene="ytfQ"
                     /locus_tag="ACHJWA_000684"
     CDS             complement(25153..26109)
                     /gene="ytfQ"
                     /locus_tag="ACHJWA_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFO4307341.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMMDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            26419..26949
                     /gene="ppa"
                     /locus_tag="ACHJWA_000685"
     CDS             26419..26949
                     /gene="ppa"
                     /locus_tag="ACHJWA_000685"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFO4307342.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(27029..27379)
                     /gene="chpB"
                     /locus_tag="ACHJWA_000686"
     CDS             complement(27029..27379)
                     /gene="chpB"
                     /locus_tag="ACHJWA_000686"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HFO4307343.1"
                     /translation="MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAADE
                     VVEEALLRLQAVVE"
     gene            complement(27373..27624)
                     /gene="chpS"
                     /locus_tag="ACHJWA_000687"
     CDS             complement(27373..27624)
                     /gene="chpS"
                     /locus_tag="ACHJWA_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin ChpS"
                     /protein_id="HFO4307344.1"
                     /translation="MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEVQVSNNQLILT
                     PISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGDEIW"
     gene            complement(27836..28177)
                     /gene="ytfP"
                     /locus_tag="ACHJWA_000688"
     CDS             complement(27836..28177)
                     /gene="ytfP"
                     /locus_tag="ACHJWA_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFO4307345.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(28180..31959)
                     /gene="tamB"
                     /locus_tag="ACHJWA_000689"
     CDS             complement(28180..31959)
                     /gene="tamB"
                     /locus_tag="ACHJWA_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFO4307346.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGILNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQIYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGLEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEIPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELTVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTLVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTEEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(31956..33689)
                     /gene="tamA"
                     /locus_tag="ACHJWA_000690"
     CDS             complement(31956..33689)
                     /gene="tamA"
                     /locus_tag="ACHJWA_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFO4307347.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            33895..34533
                     /gene="msrA"
                     /locus_tag="ACHJWA_000691"
     CDS             33895..34533
                     /gene="msrA"
                     /locus_tag="ACHJWA_000691"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFO4307348.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLHGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            34856..36199
                     /gene="paeA"
                     /locus_tag="ACHJWA_000692"
     CDS             34856..36199
                     /gene="paeA"
                     /locus_tag="ACHJWA_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFO4307349.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANI
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(36278..36484)
                     /gene="ytfK"
                     /locus_tag="ACHJWA_000693"
     CDS             complement(36278..36484)
                     /gene="ytfK"
                     /locus_tag="ACHJWA_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFO4307350.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            36809..37363
                     /gene="ytfJ"
                     /locus_tag="ACHJWA_000694"
     CDS             36809..37363
                     /gene="ytfJ"
                     /locus_tag="ACHJWA_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFO4307351.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
     gene            complement(37426..38364)
                     /gene="ytfI"
                     /locus_tag="ACHJWA_000695"
     CDS             complement(37426..38364)
                     /gene="ytfI"
                     /locus_tag="ACHJWA_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtfI"
                     /protein_id="HFO4307352.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSYTKTHSNNFFNNIHHAFKKKDWI
                     SNYDSLLTLREFFRCATQIDKSSYQVLSSKNETVNAMDKFLISFSLKDNGAEYTMTLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYAWDILQKKQSDIPKRIKGYIHQ
                     AIGDVSLGYALLDDIVSKLKRGKFELQGPGGGIKQCDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     DITKEKICEKYKSTIEAFKKEQLALPVLVRRKNSET"
     gene            complement(38576..39316)
                     /gene="cysQ"
                     /locus_tag="ACHJWA_000696"
     CDS             complement(38576..39316)
                     /gene="cysQ"
                     /locus_tag="ACHJWA_000696"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFO4307353.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGAKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIERNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            39506..41449
                     /gene="cpdB"
                     /locus_tag="ACHJWA_000697"
     CDS             39506..41449
                     /gene="cpdB"
                     /locus_tag="ACHJWA_000697"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFO4307354.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINNARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYEPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(41567..41947)
                     /gene="ytfH"
                     /locus_tag="ACHJWA_000698"
     CDS             complement(41567..41947)
                     /gene="ytfH"
                     /locus_tag="ACHJWA_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFO4307355.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            42036..42896
                     /gene="qorB"
                     /locus_tag="ACHJWA_000699"
     CDS             42036..42896
                     /gene="qorB"
                     /locus_tag="ACHJWA_000699"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313217.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO4307356.1"
                     /translation="MIAITGATGQLGQYVIESLMKTVPASQIVAIVRNPAKAQALTAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKTAGVKFIAYT
                     SLLHADKSPLGLADEHIETEKMLADSGIVYTLLRNGWYTENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDDSKTLSKLIGHPTTTLAE
                     SVSHLFNVNN"
     gene            43004..43969
                     /gene="ytfF"
                     /locus_tag="ACHJWA_000700"
     CDS             43004..43969
                     /gene="ytfF"
                     /locus_tag="ACHJWA_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO4307357.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLACVNIAELNHGLPNFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPDFSLPFGPRPLVFISLMIAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            44077..44739
                     /gene="ytfE"
                     /locus_tag="ACHJWA_000701"
     CDS             44077..44739
                     /gene="ytfE"
                     /locus_tag="ACHJWA_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFO4307358.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHARHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHNTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(44784..46196)
                     /gene="cycA"
                     /locus_tag="ACHJWA_000702"
                     /gene_synonym="dagA"
     CDS             complement(44784..46196)
                     /gene="cycA"
                     /locus_tag="ACHJWA_000702"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFO4307359.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(46505..47125)
                     /gene="fklB"
                     /locus_tag="ACHJWA_000703"
     CDS             complement(46505..47125)
                     /gene="fklB"
                     /locus_tag="ACHJWA_000703"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO4307360.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            47343..47981
                     /gene="ytfB"
                     /locus_tag="ACHJWA_000704"
     CDS             47343..47981
                     /gene="ytfB"
                     /locus_tag="ACHJWA_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFO4307361.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNYPDQVAPVAPEPIQ
                     EGQPEEQPKTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            complement(47965..48072)
                     /locus_tag="ACHJWA_000705"
                     /pseudo
     CDS             complement(47965..48072)
                     /locus_tag="ACHJWA_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(48116..49324)
                     /locus_tag="ACHJWA_000706"
     CDS             complement(48116..49324)
                     /locus_tag="ACHJWA_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFO4307362.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTASQSCGKWY
                     ISIQTENEVSTPVHPSALMVGLDAGVAKLATLSDGTVFGPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQACGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQ
                     EPTEMIQATA"
     gene            49332..49763
                     /gene="tnpA"
                     /locus_tag="ACHJWA_000707"
     CDS             49332..49763
                     /gene="tnpA"
                     /locus_tag="ACHJWA_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element IS609 family
                     transposase"
                     /protein_id="HFO4307363.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTRYRRQIFDHDATEK
                     LRTYFSKVCADFEAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGVSGRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCGGAPISAIRQYIEQQQTPG"
     gene            complement(49821..50114)
                     /locus_tag="ACHJWA_000708"
                     /pseudo
     CDS             complement(49821..50114)
                     /locus_tag="ACHJWA_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            50386..51180
                     /gene="yjfZ"
                     /locus_tag="ACHJWA_000709"
     CDS             50386..51180
                     /gene="yjfZ"
                     /locus_tag="ACHJWA_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFO4307364.1"
                     /translation="MTLPTTIYSFPAYLSRFSSTDKPVKLKFHQYARATLLSNRGRDH
                     NCDGRRTVEIHKLDLSDWQAFNKLATKCNAYDGVTMNGDNSFGWNHEATLDNIHAQKY
                     NKAYAGARLTAELKYLLQDVESFEPNSKYTIHEVVLGPGYGTPDYTGQTIGYVVTLPA
                     QMPNCWSSELPTIDLYIDQLRTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSSS
                     AAIYSGLGFERVPQERDISCARMYLTPANHPDLWTQENGEWIYLRN"
     gene            complement(51251..51700)
                     /gene="rplI"
                     /locus_tag="ACHJWA_000710"
     CDS             complement(51251..51700)
                     /gene="rplI"
                     /locus_tag="ACHJWA_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFO4307365.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(51742..51969)
                     /gene="rpsR"
                     /locus_tag="ACHJWA_000711"
     CDS             complement(51742..51969)
                     /gene="rpsR"
                     /locus_tag="ACHJWA_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFO4307366.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(51974..52288)
                     /gene="priB"
                     /locus_tag="ACHJWA_000712"
     CDS             complement(51974..52288)
                     /gene="priB"
                     /locus_tag="ACHJWA_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFO4307367.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(52295..52690)
                     /gene="rpsF"
                     /locus_tag="ACHJWA_000713"
     CDS             complement(52295..52690)
                     /gene="rpsF"
                     /locus_tag="ACHJWA_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFO4307368.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            53017..53292
                     /gene="yjfY"
                     /locus_tag="ACHJWA_000714"
     CDS             53017..53292
                     /gene="yjfY"
                     /locus_tag="ACHJWA_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFO4307369.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(53421..54107)
                     /gene="ulaF"
                     /locus_tag="ACHJWA_000715"
     CDS             complement(53421..54107)
                     /gene="ulaF"
                     /locus_tag="ACHJWA_000715"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFO4307370.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(54107..54961)
                     /gene="ulaE"
                     /locus_tag="ACHJWA_000716"
     CDS             complement(54107..54961)
                     /gene="ulaE"
                     /locus_tag="ACHJWA_000716"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFO4307371.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            complement(54971..55621)
                     /gene="ulaD"
                     /locus_tag="ACHJWA_000717"
     CDS             complement(54971..55621)
                     /gene="ulaD"
                     /locus_tag="ACHJWA_000717"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFO4307372.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(55635..56099)
                     /gene="ulaC"
                     /locus_tag="ACHJWA_000718"
     CDS             complement(55635..56099)
                     /gene="ulaC"
                     /locus_tag="ACHJWA_000718"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFO4307373.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQEAVKIGVDLLVAADVVEPRYY
                     QAILDGVEQFGPYFVIAPGLAMPHGRPEEGVKKTGFSLVTLKKPLEFNHDDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(56109..56414)
                     /gene="ulaB"
                     /locus_tag="ACHJWA_000719"
     CDS             complement(56109..56414)
                     /gene="ulaB"
                     /locus_tag="ACHJWA_000719"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFO4307374.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            complement(56430..57827)
                     /gene="ulaA"
                     /locus_tag="ACHJWA_000720"
     CDS             complement(56430..57827)
                     /gene="ulaA"
                     /locus_tag="ACHJWA_000720"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFO4307375.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIILGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            58182..59246
                     /gene="ulaG"
                     /locus_tag="ACHJWA_000721"
                     /pseudo
     CDS             58182..59246
                     /gene="ulaG"
                     /locus_tag="ACHJWA_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
     gene            59354..60109
                     /gene="ulaR"
                     /locus_tag="ACHJWA_000722"
     CDS             59354..60109
                     /gene="ulaR"
                     /locus_tag="ACHJWA_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFO4307376.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(60106..60855)
                     /gene="yjfP"
                     /locus_tag="ACHJWA_000723"
     CDS             complement(60106..60855)
                     /gene="yjfP"
                     /locus_tag="ACHJWA_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO4307377.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEK
                     WLLDDRLAVGGASMGAMTALGITARHPTVRCTASMMGSGYFTSLARLLFPPLIPETVA
                     QQNEFNNIVAPLAEWEATNHLEQLSDRPLLLWYGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            61037..61366
                     /gene="bsmA"
                     /locus_tag="ACHJWA_000724"
     CDS             61037..61366
                     /gene="bsmA"
                     /locus_tag="ACHJWA_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFO4307378.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            61515..61790
                     /gene="yjfN"
                     /locus_tag="ACHJWA_000725"
     CDS             61515..61790
                     /gene="yjfN"
                     /locus_tag="ACHJWA_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFO4307379.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(61907..63532)
                     /gene="aidB"
                     /locus_tag="ACHJWA_000726"
     CDS             complement(61907..63532)
                     /gene="aidB"
                     /locus_tag="ACHJWA_000726"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFO4307380.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDCAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(63616..64779)
                     /gene="yjfC"
                     /locus_tag="ACHJWA_000727"
     CDS             complement(63616..64779)
                     /gene="yjfC"
                     /locus_tag="ACHJWA_000727"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO4307381.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(64782..65420)
                     /gene="yjfM"
                     /locus_tag="ACHJWA_000728"
     CDS             complement(64782..65420)
                     /gene="yjfM"
                     /locus_tag="ACHJWA_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFO4307382.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(65430..65828)
                     /gene="yjfL"
                     /locus_tag="ACHJWA_000729"
     CDS             complement(65430..65828)
                     /gene="yjfL"
                     /locus_tag="ACHJWA_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFO4307383.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(65846..66505)
                     /gene="yjfK"
                     /locus_tag="ACHJWA_000730"
     CDS             complement(65846..66505)
                     /gene="yjfK"
                     /locus_tag="ACHJWA_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HFO4307384.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVVAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKENHWREAINAKTMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(66556..67254)
                     /gene="yjfJ"
                     /locus_tag="ACHJWA_000731"
     CDS             complement(66556..67254)
                     /gene="yjfJ"
                     /locus_tag="ACHJWA_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HFO4307385.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARTLEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVAATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(67273..67674)
                     /gene="yjfI"
                     /locus_tag="ACHJWA_000732"
     CDS             complement(67273..67674)
                     /gene="yjfI"
                     /locus_tag="ACHJWA_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HFO4307386.1"
                     /translation="MTWNPLALATALQTVPEQNIDVTNSENALIIKMNDYGDLQINIL
                     FTSRQMIIETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGISSVQQEEYYIVFGALS
                     LKSSLEDILLEITSLVDNALDLAEITEEYSH"
     gene            complement(67801..68532)
                     /gene="rlmB"
                     /locus_tag="ACHJWA_000733"
     CDS             complement(67801..68532)
                     /gene="rlmB"
                     /locus_tag="ACHJWA_000733"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFO4307387.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(68713..71154)
                     /gene="rnr"
                     /locus_tag="ACHJWA_000734"
     CDS             complement(68713..71154)
                     /gene="rnr"
                     /locus_tag="ACHJWA_000734"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFO4307388.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLMLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVSDWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(71193..71618)
                     /gene="nsrR"
                     /locus_tag="ACHJWA_000735"
     CDS             complement(71193..71618)
                     /gene="nsrR"
                     /locus_tag="ACHJWA_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFO4307389.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(71823..73121)
                     /gene="purA"
                     /locus_tag="ACHJWA_000736"
     CDS             complement(71823..73121)
                     /gene="purA"
                     /locus_tag="ACHJWA_000736"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFO4307390.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(73225..73422)
                     /gene="yjeT"
                     /locus_tag="ACHJWA_000737"
     CDS             complement(73225..73422)
                     /gene="yjeT"
                     /locus_tag="ACHJWA_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFO4307391.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(73504..74508)
                     /gene="hflC"
                     /locus_tag="ACHJWA_000738"
     CDS             complement(73504..74508)
                     /gene="hflC"
                     /locus_tag="ACHJWA_000738"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFO4307392.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(74511..75770)
                     /gene="hflK"
                     /locus_tag="ACHJWA_000739"
     CDS             complement(74511..75770)
                     /gene="hflK"
                     /locus_tag="ACHJWA_000739"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFO4307393.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(75856..77136)
                     /gene="hflX"
                     /locus_tag="ACHJWA_000740"
     CDS             complement(75856..77136)
                     /gene="hflX"
                     /locus_tag="ACHJWA_000740"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFO4307394.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(77213..77521)
                     /gene="hfq"
                     /locus_tag="ACHJWA_000741"
     CDS             complement(77213..77521)
                     /gene="hfq"
                     /locus_tag="ACHJWA_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFO4307395.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(77607..78557)
                     /gene="miaA"
                     /locus_tag="ACHJWA_000742"
     CDS             complement(77607..78557)
                     /gene="miaA"
                     /locus_tag="ACHJWA_000742"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFO4307396.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEIDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLGGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(78550..80397)
                     /gene="mutL"
                     /locus_tag="ACHJWA_000743"
     CDS             complement(78550..80397)
                     /gene="mutL"
                     /locus_tag="ACHJWA_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFO4307397.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRPIPENRVAAGRNHFAEPAVREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(80407..81744)
                     /gene="amiB"
                     /locus_tag="ACHJWA_000744"
     CDS             complement(80407..81744)
                     /gene="amiB"
                     /locus_tag="ACHJWA_000744"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFO4307398.1"
                     /translation="MMYRIRNWLVATLLLLCAQVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVVKRVETPTVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANNGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(81763..82224)
                     /gene="tsaE"
                     /locus_tag="ACHJWA_000745"
     CDS             complement(81763..82224)
                     /gene="tsaE"
                     /locus_tag="ACHJWA_000745"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFO4307399.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(82196..83743)
                     /gene="nnr"
                     /locus_tag="ACHJWA_000746"
     CDS             complement(82196..83743)
                     /gene="nnr"
                     /locus_tag="ACHJWA_000746"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFO4307400.1"
                     /translation="MTDHTMKKNPVSIPHTVWHADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVALIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            83742..84881
                     /gene="queG"
                     /locus_tag="ACHJWA_000747"
     CDS             83742..84881
                     /gene="queG"
                     /locus_tag="ACHJWA_000747"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFO4307401.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAMAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(84864..84917)
                     /locus_tag="ACHJWA_000748"
     CDS             complement(84864..84917)
                     /locus_tag="ACHJWA_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4307402.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT        20760 a        22939 c        20956 g        20503 t
ORIGIN      
        1 taacttctga tgattattta atagacgagc tatttccttg ttagcttcgc gttggtaaca
       61 cgccagaacc ttgttagtaa tcaggtgtaa tgaactctca tcttttcttt caaaggactt
      121 tctttcataa cggttagtcg aaccatcagg ttgtggaaat ataatggctg tatgtccttg
      181 acgagtcccc atagtagtaa tagtggaaag tgaaatttct ataatcacct tgggtgaatc
      241 cgctcgttcc ccttcaggat tattcataac aaacttcatt atctcactgc catcattatt
      301 tttgatataa gatgaggact caatatgagc catcatattt cttcccgctt cgttggagcg
      361 tttgctaaaa tccacataac ccagggtaaa tatttttacc aaaaactcaa aaaatgtctt
      421 aggattaagg atatttcttg tgttatcctg ccccccggca ttacgggcaa aattattcat
      481 tccaacacgc caggtattta aaacttcact tttttgttta aaattgtcgg ctaatgtaat
      541 gtgttgagaa taattcaaat ctgatatttt gctcatgacg aactccttga gttaataaga
      601 acgtctaagc attacaaatt ttactcacaa aaaaatatca aaaaacgctc ttttcacaaa
      661 ctcgccgtaa acctggagtt atttacggcg aaaagtcatc acgccatcag catcttcact
      721 actgctttgc gaacctgggc tggtgcaccc actgcgcaca gcggtttatg cacttccccc
      781 ggataaaaca caacaaaatc accttcatta aggataactg ttttctcatc aacgccttcc
      841 ggcaaaaatg cgatgtcttt atcagctaac cagtcggtat ccggcgtacc tgccggttgt
      901 gtgctgaagg tcatgccttc ctgacctttt aacacaatct gaatgtcgag atagcgggca
      961 tggtactccg cacggcgagc ttcgtacggc tcggtcatat cttccgagat aagataaaac
     1021 agacgattac cttcgatatc gtgcttgccc tttggcgttt ctgccgtaac gtgtgctttg
     1081 atatgctcaa tcgcctggcg taactcctgg ggtagccacg gctgaagatt atgaatattt
     1141 ccgatgatca tcttttccac tccttaatta aaacaatgtt tcatttccac cgttatacga
     1201 aaagatgatc aattcagcca gccttaacgc gtgaattatt gtcttatcac aacttatcgg
     1261 caggcgcgtt cccgcctttc ggatcaaggc cagcatcact ccttgagaat agtttttatc
     1321 gcttcccccg ccagtgcagc agttgcacag agttgttcga gggagtgccc ctctcgtgcc
     1381 ttcgcagaaa gccctgacat cacggtactc ataaaatcag tcacacattg tgcgctttgt
     1441 ggatgcagcc tggcgatata gtcataaatg gccgtttctg cggcgtgata atattgaacg
     1501 gcaatatcac gcgcttgtgg atcatgacta tgaatgcctt caagaaccat acagccagcg
     1561 cagccgccgt tttggctata tctgcgcgcc gcttctttta atacctcagc caggcactcc
     1621 cctaccggac gatcatcacg aagaatatcg acaagcggaa tagcttccgt accgacatat
     1681 tcattgagta cacggctaaa taacccagcc ttactgccaa aagccgcgta gaggctcggg
     1741 gggttaatac caagataatc agtaacttca gcaacactga cagcatcgaa acctttttgg
     1801 tgaaaaagca cttttgccgc agagactgcc tgctcaggag cgaaacgtct gggacgacct
     1861 ggaacgcgag attgtttttt agtgaccatt acaaaacttg ttgacagaaa gttaaaacag
     1921 ttttgtaatg catgttacat aataaatcaa ggagtcctta tgggcgcttt tacaggtaag
     1981 acagttctca tcctcggtgg cagtcgtgga atcggtgccg ctatcgtacg tcgtttcgtc
     2041 accgatgggg ccaatgtacg attcacctat gcggggtcga aagatgccgc tgaacgcctg
     2101 gcacaagaga cgggagcgac agcagtattt acagatagtg ctgacagaga cgctgtcatt
     2161 gatgtcgttc gtaagagcgg cgcattggat atcctggtgg taaatgcagg tattggcgtc
     2221 tttggcgatg ccctggaatt aaatgccgac gatattgatc gccttttcaa aatcaatatt
     2281 catgctcctt accatgcctc tgttgaagcc gcccggcaga tgcccgaagg tgggcgcatc
     2341 ttaatcatcg gctccgtgaa tggcgatcgt atgcctgttg caggcatggc tgcttatgcc
     2401 gccagtaaat ctgccctaca aggcatggcg cgcgggctgg cccgtgattt tggaccgcgt
     2461 gggatcacca tcaacgtcgt ccagccaggg ccaattgata ccgacgctaa tcccgccaac
     2521 gggccaatgc gcgatatgtt gcatagtttt atggctatca aaagacatgg gcaaccggaa
     2581 gaggtcgctg gtatggtcgc atggttagca gggccagaag ccagttttgt taccggcgcg
     2641 atgcatacca ttgatggcgc gtttggcgca taaccgacta cgctcaatta agcccagcca
     2701 tttcccatga tgtctgggtt ttgtttactc acgtcgtccg ctaaaagcgg ctcctggtaa
     2761 atataaatct tctgttcatg tcgtaaatct tgaggataac caaatggtag aaagaaccgc
     2821 tgttttccct gctggccgac attcactgta tgcagaacat cgttattctg cggctattcg
     2881 ttccggcgat ttgctgtttg tttccgggca agtaggaagt cgagaggacg gaacaccaga
     2941 acccgatttt cagcaacaag tcagactggc atttgataat ttgcatgcga ccctggcagc
     3001 tgcgggatgc acttttgacg atatcattga tgttacgagc tttcataccg atccagaaaa
     3061 acaatttgaa gacatcatga cggtgaaaaa tgaaatattt agcgcgccac cttatccaac
     3121 ctggacggcg gtgggcgtta catggctggc aggctttgat tttgaaatta aagtgatagc
     3181 gcgcatccct gagctgtaag caatagtgtt agccgttcgt tttcacactc cgccctataa
     3241 gtcggatgaa tggaataaaa tgcatatctg attgcgtgaa agtgaaaaag aaaaaagcag
     3301 gaaatgtctg caattattga taccgaacga cagttcccct ccagaatcac agtaaataaa
     3361 aatgcatata ttttgactta taattcaaat aaaccgtttg cgctgacaaa atattgcatc
     3421 aaatgcttgc accgcttctg acgatgagta taatgccgga caatttgccg ggaggatgta
     3481 tggttcagtg tgttcgacat tttgtcttac cgcgtctgaa aaaagacgct ggcctgccgt
     3541 ttttcttccc gttgatcacc cattcccagc ccctcaatcg aggggctttt ttttgcccag
     3601 gcgtcaggag ataaaagatg gctaatccgc tatatcagaa acatatcatt tccataaacg
     3661 accttagtcg cgatgacctt aatctggtgc tggcgacagc ggcgaaactg aaagcaaacc
     3721 cgcaaccaga gctgttgaag cacaaagtca ttgccagctg tttcttcgaa gcctctaccc
     3781 gtacccgcct ctctttcgaa acttccatgc accgcctggg tgccagcgtg gtgggcttct
     3841 ccgacagcgc caatacatca ctgggtaaaa agggcgaaac gctggccgat accatttcgg
     3901 ttatcagcac ttacgtcgat gcgatagtga tgcgtcatcc gcaggaaggt gcggcgcgcc
     3961 tggccaccga gttttccggc aatgtaccgg tactgaatgc cggtgatggc tccaaccaac
     4021 atccgacgca aaccttgctg gacttattca ccattcagga aacccagggg cgtctggaca
     4081 atctccacgt cgcaatggtt ggtgacctga aatatggccg caccgttcac tccctgactc
     4141 aggcgttagc gaagttcgac ggcaaccgtt tttacttcat cgcgccggac gcgctggcaa
     4201 tgccgcaata cattctggat atgcttgatg aaaaagggat cgcatggagt ctgcacagct
     4261 ctattgaaga agtgatggcg gaagttgaca tcctgtacat gacccgcgtg caaaaagagc
     4321 gtctggaccc gtccgagtac gccaacgtga aagcgcagtt tgttcttcgc gccagcgatc
     4381 tccacaacgc caaagccaat atgaaagtac ttcatccgct gccgcgtgtt gatgagattg
     4441 cgacggatgt tgataaaacg ccacacgcct ggtacttcca gcaggcaggc aacgggattt
     4501 tcgctcgcca ggcgttactg gcactggttc tgaatcgcga tctggtactg taaggggaaa
     4561 tagagatgac acacgataat aaattgcagg ttgaagctat taaacgcggc acggtaattg
     4621 accatatccc cgcccagatc ggttttaagc tgttgagtct gttcaagctg accgaaacgg
     4681 atcagcgcat caccatcggt ctgaacctgc cttctggcga gatgggccgc aaagatctga
     4741 tcaaaatcga aaataccttt ttgagtgaag accaggtaga tcaactggca ttgtatgcgc
     4801 cgcaagccac ggttaaccgt atcgacaact atgaagtggt gggtaaatcg cgcccaagtc
     4861 tgccggagcg catcgacaat gtgctggtct gcccgaacag caactgtatc agccatgccg
     4921 aaccggtttc ttccagcttt gccgtgcgaa aacgcgccaa tgatatcgcg ctcaaatgca
     4981 aatactgtga aaaagagttt tcccataatg tggtgctggc caattaattg cggttggtaa
     5041 taaaagtctg gctccctata atgagccaga ctttttaccg ctgtaataaa ggagaaatca
     5101 tgagcaaaac tatcgcgacg gaaaatgcac cggcagctat cggtccttac gtacagggcg
     5161 ttgatctggg caatatgatc atcacttccg gtcagatccc ggtaaatccg aaaacgggcg
     5221 aagtaccggc agacgtcgct gcacaggcac gtcagtcgct ggataacgta aaagcgatcg
     5281 tcgaagccgc tggtctgaaa gtgggcgaca tcgttaaaac taccgtgttt gtaaaagatc
     5341 tgaacgactt cgcaaccgta aacgccactt acgaagcctt cttcaccgaa cacaacgcca
     5401 ccttcccggc acgttcttgc gttgaagttg cccgtctgcc gaaagacgtg aagattgaga
     5461 tcgaagcgat cgctgttcgt cgctaatctt gatggaaatc cgggctatca tgcccggatt
     5521 aagtctgatg acaaacgcaa aatcgcctga tgcgctacgc ttatcaggcc tacgtgattc
     5581 ctgcaattta ttgaatttgt tggccggata aggcatttac gccgcatccg gcatgaacaa
     5641 agcttacttt gtctgcaatc tgaatcgggg ctatcgtgcc cagtttattc tttattgcca
     5701 gccgtaacga cggctataga accctttcac caactgggtt aatgtcatat accctgccag
     5761 aatcgcaacc agccacggga aatagcttaa cggcagcgcc tgtaattgca gataactggc
     5821 cagcggtgaa aacggcaatg cgatcccgac aatcatcacg atcacggtca tgatcattaa
     5881 cggccacgat gcacagctct gaataaacgg cacacggcgg gtgcggatca tatgcacaat
     5941 cagcgtttgc gacagtaagc ccaccacaaa ccatcccgac tggaacagcg tttgcgtttc
     6001 cggcgtgttg gcatggaata cccaccacat caggcaaaac gtcagaatat cgaagatcga
     6061 gctgatcggt ccgaagaaga tcataaagcg ccccagatcc gccggattcc aacgctgcgg
     6121 cttttgaatt tgctcgtcgt cgacgttatc aaacgggatc gccacctgtg acacatcgta
     6181 cagcaggttc tgaataagca agtgtaacgg caacatcggc aggaagggca agaaagcact
     6241 cgccaccagc acgctgaaca cattaccgaa gttagaactc gccgtcattt tgatgtattt
     6301 cagcatgttg gcgaaagtgc gacgtccctc aataaccccc tcttccagca ccatcaggct
     6361 tttttccagc aggatgatat cagccgcttc acgggcaata tctaccgcgc cgtccacaga
     6421 aatgccgata tccgcagcgc gtaaggcggg cgcatcatta ataccatcgc ccataaagcc
     6481 aaccacatgc ccttcgcgct tcagtaaggt cacaatgcgt tctttatgca tcggcgtcag
     6541 gcgggcaaac agcgtagtgc gctgtgcaag atttgccagt tcgtcgtcag atagcgtttc
     6601 aatatcacta ccaatgacca cctctcccgc gtccagcccc acttcatggc acactttcgc
     6661 tgcaactaac tcactgtcgc cagtaaggat ttttacggta attccactcg cttttaatgc
     6721 cttcagtgcc ggagcggttg tctcttttgg cggatcaagg aaagcaatat atccttcgag
     6781 gatcaggtcg gattcatccg cccgctggta atctccttca cgcgctggca ggtatttcgt
     6841 cgccaccgca accacgcgca gcccctgacg attcagcgta tcagtaaccc gcttaatctt
     6901 acgcagcatg atgtcatcga gcggcacaat ctcgccattg tgacgcacct gcgaacacac
     6961 attgaggatt tcctgcaatg cacctttgca aaccagctga tggtgctcgg tattttctgc
     7021 cactaccacc gacatccggc gacgctcgaa atcaaacgga atctcatcga ttttttgcca
     7081 acgactggcc agcgagcgcg ctgactcttc atccgtacct tcgagcaccg ctgtatcgag
     7141 caggttttta agtccggtct gataatgact attcaaccac gcgctatgca gcacgcgttc
     7201 gctggtttta ccggagatat cggtatgatt ctccagcaca attttatcct gtgtcagggt
     7261 gccggtttta tcagtgcaca gaatatccat tgcgccaaag ttctgaatag catccagatg
     7321 tttgacgatc actttctgtt tcgacagctt tactgcgccg cgcgccagcg tcgaggtaac
     7381 aatcatcggc aacatttccg gcgttaggcc taccgcgacc gaaagcgcaa acagcgccgc
     7441 ttcccaccag tcgcctttgg tgtagccatt gattaacagc accaccggtg ccatcaccag
     7501 cataaagcga atcagcagca tactgacgcg gctgatccct tgctgaaagg cattcggctc
     7561 gctttcctgc tcactaacac gccccgccag ttgaccaaac caggtattgg cacctgtagc
     7621 aatcaccatt gcttgtgccg tgccgctcac cacggtggtg cccataaaac acagcgtgtc
     7681 gcactccagc ggattgctgt gctccggctg gcgagtggta gcggcttttt ccacgggcag
     7741 agactcaccg gttaacgacg cctgagcgac gaacagatcc cgcgcctgca agatacgtaa
     7801 atctgccggg atcatatctc ccgccgccag ttttataata tcgccgggca ccagctggtc
     7861 gatcgggatc tccagccagc cattttcgcc tttgtcgtta attacgcgca gcaccgtcgc
     7921 ggtattgctg accatcgctt tcagggcatc tgccgcttta gtggaacgtg cttcctgaat
     7981 aaagttcagc aacgttgaaa tagcgaccat tagcgcgata acgcccgcgg caaataaatc
     8041 ttccgtggcg taagaaatag cgccgagaat ggtgagtaaa atattaaagg ggttgcgata
     8101 gcagacccat aaatgtaccc accacggcga cggttgttgt gcgggtaatt tattttcacc
     8161 atgttgttcg cgggcagatt ccacttccgc ctgatttaac ccttccggat gggtgtcgaa
     8221 cgttttccac aattcttctt cgggcatcac cgccattttc aggcaatgcg cacttaagga
     8281 cggcgggacc accgtattca ctgtctgctg cgcgccaggc aatggatcac gatgaaccag
     8341 acgggaaggt aaatggcgaa tgagccgggt aaaaatttct ttaaacataa ggagtccctc
     8401 cgcactgtct gaaacagtac agaaatgtct tcaggcgtgg cgaagcctta cctgaaggta
     8461 ataaagctga gcagggacgt taaaacgtca cggccttacg catccggtaa cgccgtgaaa
     8521 agacgggctt accggaaaag ttttaatctc cgtcgaggga gaggcgtggg atcaggttcc
     8581 atataacctc cggtaagtga aaaaagcgcc gcaaataata aggagaatcc aataatattc
     8641 gtagcatcta tggcgatata atacctgctg gcaactaaac caacgataaa ccagacttta
     8701 cccattgctg aatgcacggg taaagttagg ctcaaataat taaacaacac gttacaggac
     8761 aacaggatgc aaaatcggct gaccatcaaa gacatcgcgc gcttaagcgg cgtggggaaa
     8821 tctacagttt cccgggtgct gaataacgaa agcggcgtga gccagcgcac ccgcgagcgt
     8881 gttgaagcag tgatgaatca gcatggattt tccccttccc gttctgcgcg cgctatgcgt
     8941 gggcaaagcg ataaagtggt cgccatcatt gttacccgtc tggattcgtt gtcagaaaat
     9001 ctcgccgttc aaaccatgct gccagcgttc tatgaacaag gttacgaccc aatcatgatg
     9061 gaaagtcagt tttccccgca attagttgcc gaacatttgg gggtgctgaa acggcgtaat
     9121 atcgacggcg tagtgctgtt cggttttact ggcataacag aagaaatgtt agcccactgg
     9181 cagtcatcgc tggttctgct ggcgcgtgac gcaaaaggct ttgcttcggt ctgttatgac
     9241 gacgaagggg caatcaagat cctgatgcaa cggctgtatg accaggggca tcgtaatatc
     9301 agttatctcg gcgtgccgca cagtgacgtg acaaccggta agcgacgtca cgaagcctac
     9361 ctggcgttct gcaaagcgca taaactgcat cccgttgccg ccctgccagg gcttgctatg
     9421 aagcaaggct atgagaacgt agcaaaagtg attacgcctg aaactaccgc cttactgtgc
     9481 gcaaccgaca cgctggcact tggcgcaagt aaatacctgc aagagcaacg catcgacacc
     9541 ttgcaactgg cgagcgtcgg taatacgccg ttaatgaaat tccttcatcc ggagatcgtc
     9601 accgtagatc ctggttacgc cgaagctgga cgccaggcgg cttgccagtt gatcgcgcag
     9661 gtaaccggac gcagcgaacc gcaacaaatc atcatccccg ccaacctgtc ctgatcgttt
     9721 cctgaacgat aaattgtgat cttcgctgcg tttcgggaac gttcccgttt ttaaattttt
     9781 ccgcgcaata tattctgcag ccaaccaaaa atgtcatctg ccatggggct ttatgatgag
     9841 caaaataaac caaacggata tcgatcggtt gattgaactg gtcggcgggc gcggcaatat
     9901 tgcgacggtg agccactgta ttactcgcct gcgctttgtc ctcaaccaac cggccaatgc
     9961 cagaccgaaa gaaattgagc aactccccat ggtgaaaggc tgtttcacca atgccgggca
    10021 atttcaggtg gtgattggca ccaacgtggg tgattactat caagcactga tagcgtcaac
    10081 cggacaggcg caggttgata aagagcaggt aaaaaaagcc gcccggcaga atatgaaatg
    10141 gcatgagcag ttgatctctc atttcgcaga gatcttcttc ccgttgctgc ccgcgttgat
    10201 tagcggcggt ttgatcctcg gttttcgcaa tgtgatcggc gatttgccca tgagcaacgg
    10261 tcaaacgctg gcgcaaatgt acccttccct gcaaacgatc tacgattttc tgtggttgat
    10321 cggtgaagcg atcttcttct acctgccggt cgggatttgc tggtcagcgg tgaaaaaaat
    10381 gggcggcacg ccgatccttg gtatcgtgct tggcgtgaca ctggtttccc cccaactgat
    10441 gaacgcttat ctgctcgggc agcagctgcc ggaagtgtgg gactttggca tgttcagcat
    10501 cgccaaagtg ggctatcagg cgcaggtgat cccggcactg ttagccgggc tggcgctggg
    10561 cgttattgaa actcgcctta aacgcatcgt gccggattac ctctatctgg tggtggtgcc
    10621 cgtctgttcg ctgatcctcg cggtgttcct cgcccatgcg ctgattggtc cgtttggtcg
    10681 catgattggc gatggcgttg cctttgcggt acgtcacctg atgaccggca gctttgctcc
    10741 gattggcgcg gcattgtttg gcttcctgta cgccccgctg gtgatcaccg gtgtacacca
    10801 gaccacgctt gctattgatt tgcagatgat tcaaagcatg ggtggtacgc cagtgtggcc
    10861 gctgattgcg ctgtcgaata tcgctcaggg ctccgccgtg ataggcatta tcatttccag
    10921 ccgcaagcac aatgaacgcg agatctccgt gcctgcggcg atctccgcct ggcttggggt
    10981 cactgagcct gcaatgtacg gcatcaacct gaaatatcgc ttcccgatgc tgtgcgcgat
    11041 gattggttct ggtctggcag gattgctatg cggcctgaac ggcgttatgg cgaatggcat
    11101 cggcgtaggc ggcctgccgg gaattctctc gattcaaccg agctactggc aggtgtttgc
    11161 gctggcaatg gctatcgcca tcatcatccc gattgtactc acctcgttta tctatcagcg
    11221 gaaataccgc ctgggcacgc tggacattgt ttaattttct tcggggcgca attgcgcccc
    11281 ctcgcatttg caggaataac gtaatgacta atcttcccca ctggtggcaa aacggcgtta
    11341 tctaccagat ttatccaaag agttttcagg acaccacggg tagcggcacc ggcgatttac
    11401 gtggcgttat ccaacgcctg gactatctgc ataaactggg cgttgatgcc atctggctaa
    11461 ccccctttta tgtctctccg caggtcgata acggttacga tgtagcgaac tatacggcga
    11521 ttgatcccac ctacggcacg ctggacgatt ttgacgaact ggtgacgcag gcaaaatcgc
    11581 gcgggattcg tatcattctc gatatggtgt ttaaccatac ctctacccaa catgcctggt
    11641 ttcgcgaggc gctgaacaaa gaaagccctt accgccagtt ttatatctgg cgcgatggcg
    11701 aaccagaaac gccaccgaac aactggcgtt caaaatttgg cggtagtgcg tggcgctggc
    11761 atgcggaaag cgaacagtac tatttgcatc tctttgcacc agaacaggcg gatctcaact
    11821 gggagaatcc agcggtacgc gcagagctga aaaaagtctg tgagttctgg gccgatcgtg
    11881 gggtcgacgg gttgcgcctg gatgtggtga atctgatctc caaagacccg cgtttccctg
    11941 atgacctgga tggcgacggg cgtcgcttct acaccgacgg gccacgagca cacgagtttt
    12001 tgcacgagat gaaccgcgat gtgtttacgc cacgcgggtt aatgaccgta ggtgaaatgt
    12061 cctccaccag ccttgagcat tgccagcgat acgcggcact gacaggcagt gaattgtcga
    12121 tgacctttaa ttttcatcac ctgaaggtcg attatcccgg tggtgaaaaa tggacgctgg
    12181 ctaaacctga ctttgtggcg ttgaaaacat tgttccgcca ctggcaacaa ggaatgcaca
    12241 acgtagcatt gaatgccttg ttctggtgta accacgatca gccgcgcatt gtttctcgct
    12301 ttggtgatga aggtgaatac cgcgtgcctg cggcaaaaat gctggcgatg gtgctgcatg
    12361 gcatgcaggg aacgccgtat atctaccagg gcgaagagat tggcatgacc aacccgcatt
    12421 tcacgcgcat tactgactat cgcgacgtag agagcctcaa tatgtttgcc gagctgcgca
    12481 acgatgggcg tgatgccgac gagttattgg caatcctcgc cagtaaatcc cgtgacaaca
    12541 gccgcacgcc catgcaatgg agcaacggcg ataatgccgg gtttacggct ggcgaaccgt
    12601 ggattggcct gggcgataac tatcaacaaa tcaacgtaga agccgcgctg gccgatgagt
    12661 cctcggtgtt ttacacctac caaaagttaa tcgcactgcg taagcaggaa gccatcctga
    12721 catggggcaa ttaccaggat ctgctgccaa acagccctgt attgtggtgc tatcgccgtg
    12781 aatggaaggg gcaaaccttg ctggtcattg ccaaccttag ccgtgagatc caaccctggc
    12841 agccagggca aatgcgcggc aactggcagc ttgtgatgca taactacgaa gaagcctcac
    12901 cacaaccctg tgccatgact ttacggcctt ttgaggctgt ctggtggtta cagaagtaaa
    12961 tcttccctaa gccccggtaa tgccggtcat tccggggttt tgtctgtcag catgttgttt
    13021 ttgttgattt ttcaaccagc aaattcatta aaaaatttac ataacgctgt agcgcccgtc
    13081 atccgtacgc tctgcttttt actttgagct acatcaaaaa aagttcaaac atccttgatg
    13141 caaagcacta tatatagact ttaaaatgcg tcccaaccca atatgttgta tcaatcgact
    13201 ataattgcta ctacagctcc ccacgaaaaa ggtgcggcgt tgtggataag cggatggcga
    13261 ttgcggaaag caccggaaaa cgaaacgaaa aaaccggaaa acgcctttcc caatttctgt
    13321 ggataacctg ttcttaaaaa tatggagcga tcatgacacc gcatgtgatg aaacgagacg
    13381 gctgcaaagt gccgtttaaa tcagagcgca tcaaagaagc gattctgcgt gcagctaaag
    13441 cagcggaagt cgatgatgcc gattattgcg ccactgttgc tgcagttgtc agcgagcaga
    13501 tgcagggccg caaccaggtg gatatcaatg agatccagac cgctgttgaa aaccagctga
    13561 tgtcaggtcc atacaaacaa ctggctcgtg cttacatcga gtaccgtcac gatcgcgaca
    13621 ttgaacgtga aaaacgcggt cgcctgaatc aggagatccg tggtctggtc gagcagacca
    13681 acgcctcgtt actcaacgaa aacgccaaca aagacagcaa ggtgattcca acccagcgcg
    13741 acctgctggc cgggatcgtg gctaaacact atgcacgtca gcacctgctg ccgcgtgacg
    13801 tggtgcaggc acatgagcgt ggcgatattc actatcacga tctcgattac tcaccgtttt
    13861 tcccgatgtt caactgcatg ttgatcgacc tgaaaggcat gctgacccag ggctttaaga
    13921 tggggaacgc cgagattgaa ccgccgaagt cgatctctac ggcaaccgcg gtaactgcgc
    13981 agattattgc tcaggttgcc agccatattt atggcggcac caccattaac cgtatcgatg
    14041 aagtgctggc accgtttgtc accgccagct acaacaaaca tcgcaagacc gcagaagagt
    14101 ggaacatccc ggacgccgaa ggctacgcta actctcgtac cattaaagag tgctacgatg
    14161 ccttccagtc gctggagtac gaagtaaaca ccctgcacac cgccaacggt cagacgccgt
    14221 ttgtaacctt tggttttggt ctgggcacca gctgggagtc gcgcctgatt caggaatcta
    14281 tcctgcgtaa ccgtatcgca ggcctcggta aaaaccgtaa aactgcggtg ttcccgaaac
    14341 tggtgtttgc gattcgcgat ggtctgaacc ataaaaaagg cgatccgaac tacgacatca
    14401 aacagctggc gctggagtgc gcaagcaagc gcatgtatcc ggatatcctg aactacgatc
    14461 aggtagtgaa agtcaccggt tcgtttaaaa ccccgatggg ctgccgcagc ttcctcggcg
    14521 tgtgggaaaa tgaaaacggc gagcagatcc acgatggtcg taacaacctc ggcgtgatca
    14581 gcctgaacct gccgcgtatt gctctggaag caaaaggcga tgaagccacc ttctggaagc
    14641 tgctggatga acgtctggtg ctggcacgta aggctctgat gacccgtatc gctcgtctcg
    14701 aaggcgtgaa agcgcgcgtg gccccgatcc tctatatgga aggtgcttgt ggcgtgcgtc
    14761 tcaatgctga cgatgatatt tctgaaatct tcaaaaacgg tcgtgcgtct atttcgctgg
    14821 gttacatcgg catccacgaa accattaacg cgctgttcgg cggcgaacat gtttacgaca
    14881 acgagcagct ccgcgcgaaa ggtatcgcca ttgttgaacg tctgcgtcag gcagtggatc
    14941 agtggaaaga agaaacgggt tatggtttca gtctctacag cacgccgagt gaaaacctgt
    15001 gcgaccgctt ctgccgtctc gatactgctg agtttggcgt ggtgccgggc gtgaccgata
    15061 aaggttacta caccaacagt ttccacctcg atgtggagaa gaaggtgaac ccgtacgaca
    15121 agatcgactt tgaagcgcct tacccgccgc tggcgaacgg tggtttcatt tgctacggcg
    15181 agtatccaaa cattcagcac aacctgaagg cgctggaaga tgtctgggat tacagctatc
    15241 agcatgtacc gtattacggc accaatacac cgattgatga gtgctacgag tgtggcttta
    15301 ccggtgagtt cgagtgcacc agcaaaggct tcacctgccc gaaatgtggt aaccatgacg
    15361 cctcccgtgt gtcggtaact cgccgcgtgt gcggatattt aggtagcccg gatgcacgtc
    15421 cgtttaacgc cggcaagcag gaagaagtta agcgccgcgt taaacatttg ggaaatgggc
    15481 agataggttg atctgcacta agcaaatgcc ggatgtgatg tgaaagcgtc gcatcccgcg
    15541 ttgattgccg gatgcggcgt aaacgcctta tccggcctac atttggcaac ggctgtaggc
    15601 ctgataagac gcgacagcgt cgcatcaggc gttgattgcc tgatacagcc tacacgcgga
    15661 ctgataagcc aggcgaaata atgaattatc atcagtacta tcctgtcgac atcgtcaacg
    15721 gtcccggcac tcgctgcacc ctgtttgtct ccgggtgtgt tcatgaatgc cccggttgct
    15781 ataacaaaag cacatggcgg gtaaattccg gtcagccatt tactaaagca atggaagacc
    15841 agatcattaa cgatctgaat gacactcgta tcaaacgcca gggtatttcc ctctccggcg
    15901 gcgatccgct gcatccgcaa aacgtgccgg atattctgaa actggtacaa cgcatccgcg
    15961 ccgagtgtcc gggtaaagac atctgggtgt ggacaggcta taaactcgac gaactcaacg
    16021 ctgcgcaaat gcaggttgtt gatttgatta acgtgctggt cgacggcaaa tttgtgcagg
    16081 atttaaaaga cccttccctg atctggcgcg gcagcagcaa tcaggtggtg catcatttgc
    16141 gatgataagt tccctgttac ctgagtgcag gcaacaggga aatgaggaat taacgatact
    16201 tcttgtgata tgaattgcgg gtcgttttca gttgctctgc agcagcctgc gcttctttta
    16261 ctttaccttc atttgccagc ttcagcgcgt cgtcaatctg accgaccaga atgtcgaaac
    16321 cgtggcggaa atctttcatt tccgggctgt ccggtgattt atcttcgagc ttcggcggcg
    16381 ttgctttttg cgcatccagc gctgcggcgc gcatcttcgt taacgcgtct ttgacttgcg
    16441 ccgcgttatc ggctttttcg accactttta aattgtcgtt gagggtttcc atattgtctt
    16501 caagatcagc agcaaacgac gccgaactga ataccaacga ggagactgca agaatagcta
    16561 acaggctttt tcgcattgct cacttccttt tttatttaca cttcatcctt cacactgcct
    16621 catcgacgac tacgctcatt cgaccgggtc acgtactgcg agtacgctcc ccggccctca
    16681 ttcactttcc gtctcgatgc agtgcgaatg attttgtgta attttttagt ttatagcgcg
    16741 gcaggtcgcg ccagtttttt tactgtccgg cgattttcat ctccggcaac agcacagaac
    16801 cacactgtat attactgcgt gtttcaatat cgttaccgac ggtgacaata ttgcgccaca
    16861 tatcttttaa attacctgcg atggtgattt cgctaaccgg atactgaatt tcgccgtttt
    16921 ctacccagaa gcccgccgca ccacgggaat aatcaccggt aattgcactc acgccctggc
    16981 ccatcaattc cgtcaccacc agcccggtgc ccatctcttt gagcatctgc tcgaagctta
    17041 gaccttgtcc ggcaatccgc cagttgtgaa taccgcccgc atgtccggtg cttttcagcc
    17101 ccagtttccg cgccgagtag ctggtcagca gccactgagt caggatgcca tctttaataa
    17161 tatcgcgacg ctcggtgcgc acaccttcgc tgtcaaatgg cgtcgatgcc agccctttca
    17221 gcagatgcgg atgctcttca atggtcagcc agtccggcag aatttgttta cccagcgaat
    17281 ccagcaggaa ggtagattta cggtaaaccg atccacccgc tatcgccccc accagatgcc
    17341 caaaaagccc ggttgccact tcattggcaa aaatgaccgg cgctttcatg gtggagagtt
    17401 tacgcggtga cagacgcgat aaagtacggc gagcgcaatc ggccccgacc cactctggcg
    17461 tctgcaaatc gctcatcgcg cggccaatgg tgtaggcgta atcacgctcc atatcgccat
    17521 tttcttcggc aattacacag ctggagagcg aatgacgcgt tgagcagtaa ccctgcaaca
    17581 tgccgtggct gttgccaaaa actttgacac cgtagtggct gttaaagctg ccaccttcgg
    17641 tattggtgat gcgtttgtcc gcctgcaatg ccgcctgttc tgcgcgggcc gccagttcaa
    17701 tggcttcatc cggggaaact tccgcagggt ggaacaagtc gagatccggt gcgtcaaagg
    17761 ccagcagctc tttgtctgcc acgccggcac agggatctgg cgaggtataa cgggcaatat
    17821 ccagcgccgc ctgtacagta cgggcaatgg cctgcgggct taaatcggtg gatgatgcgc
    17881 tacctttgcg gttctggtga taaacagtga tccccagcgc gccatcgcta ttgaattcaa
    17941 cattctccac ttcaccataa cgcgtgctta cgctaatgcc ggtggtcttg ctgacggcaa
    18001 cttccgcacc gtccgatttg cctgaggcca actccagcgc agtggaaact gcttcttcca
    18061 gaatcttgcg ctgcgcttca acttgagaga ttactttcat tgcaagtgcc ataatgtaga
    18121 gagtttctct gaagtctaac agagaaccgt ttttcagtgc gcatcttaac tggtaacatt
    18181 agcctctttt tttaaggagc ctgagatgac taagcagccc gaagactggc tcgacgacgt
    18241 tcccggtgat gacatcgaag acgaagacga tgaaattatc tgggtcagta aaagtgaaat
    18301 taaacgtgat gccgaggagc taaaacgcct tggcgcggaa attgttgatc tggggaaaaa
    18361 cgcgctggat aagatcccgt tagatgcgga tctgcgcgcg gctattgagc tcgcccagcg
    18421 tattaagatg gaaggtcgcc gccgccagtt gcagctcatc ggtaagatgc tgcgccagcg
    18481 cgatgtagaa ccgatccgtc aggcgctgga taagctgaaa aaccgtcaca accagcaggt
    18541 ggtgctgttc cataaactgg aaaacctgcg cgatcgtctg atcgatcaag gtgatgacgc
    18601 catcgctgag gtattaaatc tgtggcccga tgccgatcgt cagcaactgc gtactctgat
    18661 ccgcaacgcg aagaaagaga aagaagggaa taagccgccg aaatccgcac gccagatttt
    18721 ccagtatctg cgcgaactgg cggagaatga agggtaattt tcgttgttga tgtgagtatg
    18781 gtttgccgga tgcggtgcac ctgtaggcct gataagacgc atcaagcgtc gcatcaggca
    18841 ctatgctcaa atgccggatg cggcgtgaac gccttatccg gcctacgcac actatctcag
    18901 gctgaggccg aattactgcg cagcttccgc cttcttcgcc agaccatcca gtagtttctg
    18961 atggatccca ccaaaaccgc cgttgctcat caccagaata tggtcgccag gctgagcggt
    19021 tttcaccacc atatctgcca gcgtatccac atcgccactc cagtgtgcag gctgaacgca
    19081 ggcttctgcc acttctgcca cctgccacgg aatatgcgcc ggttgcagca ggaagacttc
    19141 atcggcacga cctaatgaag gtgccagatc gtctttgcag atccccattt tcatggtatt
    19201 cgagcgcggc tccagcacag caataatgcg cgccgtacca ccaactttgc cacgcagcgc
    19261 cgccagcgtt gccagaatcg ccgtcggatg atgggcaaaa tcgtcatata ccgtgacgcc
    19321 attcgcttca ccacgcaact ccagacggcg gcgagcgtta ataaacgaac ccagcgcgtt
    19381 agcggcatct gccggcgcta caccaacatg gcgagccgct gcaatcgcca tcaggccatt
    19441 gtgcatatta tgttcgccta ccagcgacca tttcacttcg cccacttttt cgccatccag
    19501 caaaacttcc cattcggaag catcggtggt cagctttttc gcctgccagt gaccctgctc
    19561 acccaccagc tcctgctcgc tccagcagcc catcgccatg gtctgtttca ggttgatgtc
    19621 attttccggc cagataatac gaccctgccc cggaacgata cgcaccagat ggtggaactg
    19681 tttctggatc gctttcaggt cgtcaaagat atcggcgtga tcgaactcaa ggttgttgag
    19741 gatcagcgta cgcgggcagt aatggacaaa tttagagcgt ttgtcgaaga aggcgcagtc
    19801 gtactcatcc gcttcgataa caaagaagtc gctttcgccc agacgcgccg aaacctcaaa
    19861 gttccccggc acaccgccga ttacaaagcc cggtttgtaa ccacactgtt ccagaatcca
    19921 agtcgccatt cccgcggtgg tggttttgcc gtgtgtaccg gcaacggcca gcacccagcg
    19981 gtcgcgcagc acaaaatcgt gcagccactg tggacctgac atataaggga tgtttttttc
    20041 cagtaccgct tccacacacg gatttccacg ggtcatggcg ttgccaataa tcaccagatc
    20101 cggctgcggg tcgagctggc tggcatcgta accctgaatc agctcaatgc cttgcttctc
    20161 aagtaaggtg ctcatcggcg gatacacatt ggcgtccgaa cccgttactt catggcctaa
    20221 ctggcgcgcc agcatcgcca gaccgcccat aaacgtgcca caaattccta aaatatgaat
    20281 gcgcatgcgt cactatcctt ttttaatctg ccgctcattt tacgcatatg tcgggcaagt
    20341 gagaaacgca tttcaggata atccgtaatt tgctggcgcg attcacctga gcgcaacatt
    20401 gagattattt gttaagattg ttgcggtcgc tttactccat aaacattgca gggaaagttt
    20461 tatgaaaacg ttaggtgaat ttattgtcga aaagcagcac gagttttctc atgctaccgg
    20521 tgagctcact gctttgctgt cggcaataaa actgggcgcc aagattatcc atcgcgatat
    20581 caacaaagca ggactggttg atatcctggg tgccagcggt gctgagaacg tgcagggcga
    20641 ggttcagcaa aaactcgact tgttcgctaa tgaaaaactg aaagccgcac tgaaagcacg
    20701 cgatatcgtt gcaggcattg cctctgaaga agaagatgag attgttgtct ttgaaggctg
    20761 tgaacatgca aaatacgtgg tgctgatgga cccactggat ggctcgtcca acatcgatgt
    20821 taacgtctct gtcggtacca ttttctccat ctaccgccga gttacgcccg tcggcacgcc
    20881 ggtaacggaa gaagatttcc tccagcctgg taacaaacag gttgcggcag gttacgtggt
    20941 atacggctcc tctaccatgc tggtttacag taccggatgc ggtgttcacg cttttactta
    21001 cgatccttcg ctcggcgttt tctgcctgtg ccaggaacgg atgcgcttcc cggagaaagg
    21061 caaaacctac tccatcaacg aaggaaacta cattaagttt ccgaacgggg tgaagaagta
    21121 cattaaattc tgccaggaag aagataaatc caccaaccgc ccgtatacct cacgttatat
    21181 cggctcactg gttgcggatt tccaccgtaa cctactgaaa ggcggtattt atctctaccc
    21241 aagcaccgcc agccacccgg acggcaaact gcgtttgctg tatgagtgca acccgatggc
    21301 attcctggcg gaacaagcgg gcggtaaagc gagcgatggc aaagagcgta ttctggatat
    21361 tatcccggaa accctgcacc agcgccgttc attctttgtc ggcaacgacc atatggttga
    21421 agatgtcgaa cgctttatct gtgagttccc ggacgcgtaa tctcctgcgc ctctccacac
    21481 ggagaggcga ttttattgct gcgcaatgtt gactcttgtc acaggcgaac tctgacgatt
    21541 ctcccacagc accgttaatc cacgctgtaa tgctataaaa ataaacaaca aaataccgat
    21601 ggcgattttc gtccaccatg agctcagcgt accatcaaag tttatgtaag tctgaatcag
    21661 tccctgaatc gccacgccaa aaagcgtccc taataccgtt ccaacgccac cgctcaaaag
    21721 cgtaccgcca atcaccactg aggcgatagc gtccagttcc acacctacgc ccgccagcgc
    21781 gtatccggcc tgggtataaa tcgagaagac aatccccgcc agtgtcgcca gtccggtaga
    21841 gagcatataa atgcgaatag ttgtgctgcg agtggaaatc cccatcaggt ttgccgacgt
    21901 cgcattgccg ccaatggcgt acacctgatt accaaagcgg gtacgatgcg cgaggaatat
    21961 gccgataacc accaccgcca gcatcagcag ccccatcgca cttaagcgac cgccgccagg
    22021 aattttccat gcaaggcttg agagcgtgtc ataaacagga tggtttatcg ggatcgactc
    22081 ttccgaaacg aggtagctga cgccgcgcaa aaagaacatt cctgccaaag taatgataaa
    22141 tgccgggatc ttcagggcgt cgatcaaaag ccccataaat gcgccaaagg cacagcccat
    22201 taccagcacc agcggaaatg ccagcaacgg cgagaggccg aaatcgccaa tcacttttgc
    22261 cagaaacacg ccggtaaagg cgatcaccga accaacggag agatcgatcc caccggagag
    22321 gatcacaaag gtcatgccaa cggcgattat cccaagaaag gcattatcgg tcaggatatt
    22381 gcagatcact cttgtggaag caaaaccggg aaactgggtc aggcagtaga gataacccag
    22441 cacaaagacg ccgatggtga tcatcagtgg caaattacgt tttatcacgg ctacgcactc
    22501 ctttaatcag actgataaag cgttgcgact ggacaatcag cacgcaaagc accaccaccg
    22561 ctttcaccac ctggttcatc tctggcggaa agcccgaaag caaaattccg gtgttcatcc
    22621 cctgaataat cagcgccccc accaccgaaa gcagtaggtt aaaacgtccg cccatcagcg
    22681 aaccaccgcc aatcaccacc gcgagaatgg cgtccagctc cagccataat ccggcgttgt
    22741 tggcatcggc accgcgaata tccgccgcca cgataatgcc cgcaatcgcc gcacacagcc
    22801 cgctcaacac gtaggtaagc atgacgatga tccgcgtgtt taccccggca ttttttgccg
    22861 cccgaatgtt gataccaacg gcttcgataa acatccccag cgccgttttg cgggtcaaca
    22921 gccagaacag gataagcgtc agcaccgcga taatgaccgg cgttggcagg aacaacagcg
    22981 atccactgcc gaaccatgag agatccggcg agttaaacgt gatgatctgt ccggaggtga
    23041 tcagttgcgc cacgccgcgc ccggcgacca tcaggatcag agtggcgaca aacggctgaa
    23101 ttttgaggat cgctaccagt atgccgttcc acaatcccgc caggatgccg gtgcccaggg
    23161 cgcttaacaa aacaatcggc aggctgaatc ccgcgaccgt catcgcagcc gttgtggctc
    23221 cggcgatagc catcaccgcc cctacggaga gatcaatccc acctgttgcg atcaccagcg
    23281 tcataccaat cgccagtagc gcaacggggg cggcgcgatt aagaatgtcg atagggctac
    23341 cgaacaaacg cccatcctga agcaccacct gccagaaatg cggggccacc aggctatcga
    23401 ccagcagcac cagcaacagt gccgccagct gcggcattcc cgttggccag cgaaagcgcc
    23461 tttttggcgg cgtagtgtcc gggagagatt gaggcatcac actgttctcc ttacgccgca
    23521 atggcattca tgatcgccgg aacggaaagc tctgccagcg ggatctccgc cacctgtttg
    23581 cgatcgcgca tgatgatcac ccggtcggca tagcccacca gttcttccag ttcggaggag
    23641 atcaccagca gcgccagacc atcggcgcat agcgtttcaa tcaggcggat gatctcggcg
    23701 tgggcaccaa catcaatgcc gcgggttggc tcatcgagga tcagaaattg cggtcgggtc
    23761 agtagccaac gtgaaagcaa cactttttgc tgattgccgc cggagagaaa ttcaatcggt
    23821 tgttcagttg aaggtgtgcg aatgccaagc tggcggataa agcgttcggc aatctcttgc
    23881 tgttctttgc gggaaatggg acgtagccag ccgcgctggg cctggagagc gaggatgata
    23941 ttttcccgca ccgaggcggc agcgatgatg ccatcggttt tcctgtcttc cgggcagaag
    24001 ccaatgccaa gtaccgaagc ctgatgtggc gatcgcaggt tttgcggttt gcctttgatc
    24061 aacgccgtgc cgctgtcagc aggtttgata ccgaagatca cttcggcggt ttcggtacgt
    24121 cctgatccca gcaatccagc cagaccgacg atctcgccgg ggcgtacttc gagatcaaac
    24181 ggtgcgatcg ttcctttttt gccgtaattt ttgaacgcgg caacgggttt gtcgctcaac
    24241 aatgttcgcc cggcacgctg tagcgcgtgg gtatccagct cgcgccccag catcattttt
    24301 accagttcga tctgcggtag ctcgcacgtt tcccgacagc ctacgaaact gccgttgcgt
    24361 aagacggtga tccgatcgct gacctgatag acctgatcga gaaagtgagt gacaaagatc
    24421 aggctgacgc cgcgatcgcg caactgacgc atcaggtcaa acagtaactc cacttcctgg
    24481 gtgtcgagac tggcggtggg ttcatcgagg atcagcactt tggcagagag atcgatagcc
    24541 cggcaaatag cgacgatttg ctgcatcgcg actgaaaagc ggttgagcgg ttcgcgcacg
    24601 tcgagggaga aaccgtaaga tgccatcagt tcggtggcgc gcttttccat ctctttgcgg
    24661 cgtagaaggc cgaagcgttt gggttcgcgg cctataaata gattatcagc gaccgacata
    24721 ttgggtagca ggttgacttc ctgatagacg gtgccaatgc cgagttgttg cgcgtgggcg
    24781 gtatttttcg gtgagatagc ctggccttcc agccagatgg tgccgcgatc agcgtggtat
    24841 acaccagtta atgctttgat tagcgttgat tttcccgccc cgttttcacc gagcagcgcc
    24901 atgatttcgc cacggcgcag gctgaaatca acgttgtcta acgctttgac gccggggaaa
    24961 aatttactta atccttcggt gcggaggatc tcctggtgtt ggtcggtcgt catggtttcc
    25021 cccggtgtaa agcgtgctcc cagtaggcat gataagacgc ggtaagcgtc gcatcaggct
    25081 tcagtgcacc atcgccggat gcggcgcgag cgccttatcc ggcctacgaa tcggcatcag
    25141 gcatagcaac cctcaatacc ccatattttt cttcttctct aactcttctt ttgcggtatc
    25201 aggcaggtaa agggtggatt tggttaacgt cagcttttca ggcatggtgc cgtctttttt
    25261 gtatttctcc agcgcatcga aagcgggtcc cgccatgttc ggcgtcagtt caacactggc
    25321 gttcgcttcg ccatccatca tcgctttgta gatgtccggt acaccgtcaa tggaacccgt
    25381 gaggatatct ttgcccggtt tcaggcccgc ttctttaatt gcctgaattg caccaatcac
    25441 catgtcgtcg ttatgggcgt aaaccatgca gatgtttttg ccgttgtttt ccgctttgat
    25501 aaagctctcc atgacttctt tgcctttact gcgggtgaag tcacctgact gcgagcggat
    25561 gattttgata tttggcgcat tcttaatggc ttcggcaaag cctttcttac ggtcaatggc
    25621 gacgctggcc ccaacggtgc cctgcagctc caccacgttg catggtttgc cattcacttc
    25681 ttttaccagc cagtcaccga tcaacttacc ttcgaggatg ttgtcggcag taacggtggt
    25741 catatagaga gatttgtctt tcacatcgat ggaacggtcg agcaagaaga ccgggatttc
    25801 ggcatctttc gcctctttta ataccggttc ccaacctgtc gcgaccaccg gagcgataaa
    25861 gatcgcatcc accccttgtg caacgaagga acgtaccgct ttaatctggt tttcctgctt
    25921 ttgctgacca tcggcaattt tcagcgtaat tccgcgtttt tcggcctcac ttttcgccac
    25981 attggtttct gcggcgcgcc agccggattc cgatccgacc tgcgaaaatc ctacggttaa
    26041 tggagcggcc aacgccatag acgacatggc tgccgagact gcagagacta caagtaagcg
    26101 tttccacata gggttgtcct cgtcggagta attattattg gtgaaaagat gttcgcgaaa
    26161 aaactataga caattcgtta tgtaacggat tgcgttacat cacacttcaa aaaagtaaat
    26221 aaaacgttaa tcacaagttt gtaatcgctt tcatctcact atgaaaaatg cggctacggt
    26281 tatggatttt cctgctctgc ataccgtctt aaaactggcg aaaaaggaaa atgaagacga
    26341 aaacaagcga agacattcgg cgcgagttgg ctataatact cggcacttgt ttgccacata
    26401 tttttaaagg aaacagacat gagcttactc aacgtccctg cgggtaaaga tctgccggaa
    26461 gacatctacg ttgttattga gatcccggct aacgcagatc cgatcaaata cgaaatcgac
    26521 aaagagagcg gcgcactgtt cgttgaccgc ttcatgtcca ccgcgatgtt ctatccgtgc
    26581 aactacggtt acatcaacca caccctgtct ctggacggtg acccagttga cgtactggtc
    26641 ccaactccgt acccgctgca gccgggttct gtgatccgtt gccgtccggt tggcgttctg
    26701 aaaatgaccg acgaagccgg tgaagatgcg aaactggttg cggttccgca cagcaagctg
    26761 agcaaagaat acgatcacat taaagacgtt aacgatctgc ctgaactgct gaaagcgcaa
    26821 atcgctcact tcttcgagca ctacaaagac ctcgaaaaag gcaagtgggt gaaagttgaa
    26881 ggttgggaaa acgcagaagc cgctaaagct gaaatcgttg cctccttcga gcgcgcaaag
    26941 aataaataag ttcttctggc gtaataaccc tgaacgccgg gcttcggtta gtaagggttt
    27001 ttttatgccc gcgataaata aacacacctt attccaccac cgcctgcaag cgtaataacg
    27061 cctcttccac cacctcatcc gcagccagac caatacgctt tgccagccgg gcgcgtagat
    27121 ccatcatccg cacctgatta accagcacca cgccgtgcac atcgccttct tcgcaatgta
    27181 aaggaacgct aaatccggca taacgggcaa aatttccacc ctgcgtaatg ggggccacca
    27241 gcgtcattcc cagttgatta aaggcttgaa cggagagcac aagcgcaggt cgaccagcac
    27301 cttgctgttc atggccgctt gctggatcaa agccaaccag cacaatgtct ccccgttcaa
    27361 attcactttt ctttaccata tttcgtcacc cgcaggggtg gatttacccc agacatcctg
    27421 ctcgctaagt tccgcggcgt tcatgtcaca ctgtgccagc agttcatcaa gcgagtagcg
    27481 cctggagatg ggtgtcagaa tcagttggtt gttgctcacc tgcacttcca cgctctgccc
    27541 cggctgtaag ttaagttctt tcattacgat attgggaatg accatacctg cactgttccc
    27601 ccatcttttt atggtaatac gcatctgcac ctccttaagt tatacaaagt ataactcacg
    27661 gtgctacagg aaacgtttta cccctgcgca tttgtgaatg ccagcagagt tttttatgcg
    27721 ttaatagata tggaaagcgg ctcggagaga aaagcaaaag gtgagggaat tacaaaacag
    27781 aatgcgagtc gtctcgcaaa aaacaacgcc acccgaaggt ggcgtatgca tagggttact
    27841 tatccctgtc taaccagtcg ccgctttcaa ttagctttaa tccatcgacg ggtcgttgat
    27901 aaacgtacat ccatgcactc ccgtacggcg tctgaatcaa ctggcgcgcg tattcaccgc
    27961 ccctggtgcg caaggcatca agttcggcca gcgtggcgtt gtcaatacga taaacttcac
    28021 cgtgtaccgt tccgttcccc ggaactgcgc ctggatagtg gcccaggcta tacaactggt
    28081 agttatcgat actgaaatcg cccagtaact gggcattggt catccagtga ctgttgcctt
    28141 gtttgtggcg taaactgccg tagacaaata ttcgcattgc taaaactcga actgatagag
    28201 caaatccagt gcctggtcta caccagacac ggcttccaga tatagcttag gcatcaggcg
    28261 ataacgtaac gtgagtgttg ctatagagtc aaatataccc acgccgtatt tcacttgcag
    28321 acctggcaat acatagccgc tgaccactac ctgggaggag tcgcctactc cctgggtgtc
    28381 gagcgctaaa ttgcttacgc caaacgtctc gccgatttta cctacaatct ggccactttg
    28441 cgcaaccccc agaccaatca gcatcgaggt cattgccgca ctgtcgctct gatcgctctc
    28501 cagcccttgt ccacgtagca aataagacaa ggcagcttgt tgcgacatcg ccgggtcaga
    28561 gaagatctcc gctttcggtt cgtccgccag accagtgacg cgaactccgg cgattacgtc
    28621 gtcttctgta gcatccgggt tacgaatagc ttcaatatta agatacggtt gatccggcgg
    28681 gccagagaac agtaactcac ctttacgcac aatcagatcc tgaccatagg catggaagcg
    28741 cccttcaggg atgttgatct gcccgttcag gcccagccct tgtttgtcct gaacgacgtt
    28801 aagatcgccc gtcagccgcg ctttcaggcc aaaggcgtca atgcgcacat tgttgccaac
    28861 gtggacaatc aggttactgt taatcggaat cgacgccgtt ttcggctctt ccggttgcag
    28921 gttatcgtta agcatcacca catcgctgga gacgcctact gcgctttccg gcagatcgtg
    28981 caccacgatg cgcgcccaag ggacatccac gcgaccatcg agggtaaaca ggtttggtgt
    29041 agcctcgaat acaacatctg gcgatacatc cattcgtacc atcggcggca cggtgatccg
    29101 cactttactg cctttcgccg ttacccgcgc ccgccagttt tcaatttggc tccagtcggc
    29161 atcaccgttc aggtagattt caccctgctg ggttcgtact gtaccggcaa gcgtcgagcg
    29221 cataccgtta aagttgaccg caagctggct cggttgcata tcaaacggca taaagttgcc
    29281 atcgatatcc acacccgtaa cctgaagctg accaaacaac tgcgggcttt gcacatcacc
    29341 acccagacgc aagttggcac tcaccatccc cgctgctttt tccccacggg taaagatggg
    29401 gtttatcatc gcaaggttga agttacggat attgacgttg ccaccaagat tacggcggcc
    29461 ttgcggatcg gtcacctgca cctgtccatc aaactggccg ttattggtca ggcggatggt
    29521 ccagcccaat tcggcacggt tgttacgcaa ttccgccgtc agattcagtg tctgaaacgc
    29581 cacgggcagc gccgcatcgt tgacggtttg cgttacctgc acgttacgcc ccgaaagggt
    29641 gatactgccc tgcggcagcc cctcttcggt ggtgtcccag gcaacgtccg ctttacccgt
    29701 gaagataccg ctggcctgag tggtttctgg cataaacggt ttcagcatgg caaggtcgaa
    29761 gcggttgaga ttcaccaccg cacgcccttc ggcccccgca tcgatagttt gcggcacgca
    29821 cagttccgca ttcgggttaa gccagcagtg tggcccgatg ctgatttttt gctccttatt
    29881 gcggtaatcc agcgcaatat cgcgggtcag cgaccacggg ccgaccagcg tctggaagcg
    29941 ggtattgcta agagttccct tccagcgttc ttctttgcga tcaaaacttc ctgccagatt
    30001 aagctgcccg gaaaccggct cgccctgaat ccgcaactgt aactcgtgct gtttttcgct
    30061 gcctttggca ttcagggtga cgaggttgat atttacatcc ggctgtgaaa tttgctcaac
    30121 gcgcacgtcg agtttacccg caatctgatc ggtggatttg atatcgcctt ccacgcgaac
    30181 ctgcgccacg gttagctcct gccagcgcag gccgcgcgcg gtgatgtccg ccagtagttg
    30241 tggcgcttcc accgtgccgc gaactttcac cagccctttc gctgtaccgc caagccccgg
    30301 cagcgcgtta tccagccccg gcgcgttgat ggtggcatca agattgagat cttttacccc
    30361 cagctcgcct ttcacttcgg cactgtttgg ccccagttcc agatgaagcc ctgggatcat
    30421 ccactgcata taactgttgc ctttcagcgt gccgtcaacg ttcactttgt tctgtttgac
    30481 gttaccggta agcttcagtt ctggcacctc catctgccag gtgccgccgt acaggctacc
    30541 gcgggtttta atcaagccat tgagtttcga cggccactct gggatctctt tggcggtgtt
    30601 aatgccgtta agcgttagct caccgcgcca actaatggcc tgctgccagt caagcaacgc
    30661 cttgagttca gttttccctt ccagcgccgc gacggtgagt ttgtcgagat tgacctgctg
    30721 ttcattacct ttggcatcaa gggtaatggt ggctggcggg atctccagtc ccttcactgc
    30781 cgtacgcata gagagcgtgt aatcggtcat tttgccggta agtttcagtt tcagatcatc
    30841 cgcctgatac tgcttctcac cagtgaacgg ccagtaaatc tgtttgctgt tcacttccac
    30901 gttgagcggc aatccggctt ccgccagtcg cgtctgggcg cgtaaatcca tatccaccgg
    30961 accggaaaga ttaacgccaa tctccagctg ttcgcgcagc gcgccgccca ctttcagctt
    31021 caccttttca cctttcaacg gctccacgtt cagtgtgctg ttgagggtga tatccaccgg
    31081 ccagttgtct gacagctgcg ccgtgccgct ggcgttgagg atcccttgat tggaatcgat
    31141 atccagggcg tccagtttag tattgccgtc aatgctgctc actttcagca gcatggtgcg
    31201 cacggtaatg tccgtatcgc ccgtcacgcg cagttgctct cccttaaact cttcaatgtt
    31261 caggttgagt ggcagatgca cgtcggtcat ttccggcaat accgggcgag aaaagagatc
    31321 tttgagtgtt tcgccgagag gcttttcctc cggctgcgga ttttcaattt tcggttcgac
    31381 cacttcttcc tgcgccactt ccgccacttt cggcagagca atcagcaggc ctttcagcga
    31441 cgtcggtttc agggtcaggg ttttctcctg ccagttcagg ccggaggtga agtccatcac
    31501 cgataccgtg gtgtcatcaa tcttgatgtt gacgttgtcc agtgccaccc gtgtcagggt
    31561 gatgggatac ggcgtggaga gatccagcgg accgctatct tcctcttctt caacctgttc
    31621 agaaggaggc atttttttac tgtcgatgtt gacctgaatg tctttcagcg ccaagtcatt
    31681 aatacaaaca ctgctgttcc acaggcactc aagaccgacc gccagatgca gattacccgc
    31741 ttttaccgcc acgcctggct gctcataacg aacgtcagac aaggtgagat cgcgccagcc
    31801 gccggtgacc ttgccaatat ccagtcctgg cacccagcga tccgccgctt taaataccag
    31861 atgcaggccg ctggtggtgc ccaccagaaa cgccaccgat cccagcaaca gtaagataac
    31921 gataaccacg ccgaggctga tttttttcca taaactcata attctggccc cagaccgatg
    31981 taaaactgta acccgtgttc gtctttatcc gcgaccggta cggcaaaatc gagtttgatt
    32041 ggcccgaccg gcgattccca gcgtacgccg accccggtac cggttttaaa gtcgctgcgg
    32101 cgaatatcgc ttaccgcttc gccactatcg acaaacaccg cgccccacca ttttccggtc
    32161 acgttgtact ggtattccag cgatccggtt atcaacttcg aggccccttt caggtcaccg
    32221 ttagcgtatt tcggggcgat agatttgtat ttgtagccgc gaatactgcg atcgcccccg
    32281 gcgaagaaac gcagatccgg cggtactttg tcgaaatcac cggtttcaat ccagcccagc
    32341 gtgccgcgtg tcacaaaacg atggcgatcg tacagtgtgc ggatccagac gttctgcgcc
    32401 tggaaaacgg agaaatcgac atctgaaccc caggccgtgt tggagtagtc gatagagtag
    32461 cgttgcgagt cgccccaggt tggcatcagg ccaccacgag aacgcgtgcg gctaatcatc
    32521 accccaggat aaaacagcat cgtggtgttg gtaatttcac cctgagtaaa gtggtcgaga
    32581 ctccagcgca ggttaatggc acgctgccag ccgctggaga gatcccagta gcgagaagcc
    32641 accagcgtag tggagtcaga ctcggtatcg ttcaggtcag tgcgcttaaa accgccctgc
    32701 accaaataat attgttccag tggattcttc agcagcggca ttttatagct gaagtcgagg
    32761 gtctgttccg gcgcggaaat actggtactg gtggtcagac tgtgaccgta tgagttcatc
    32821 cacggttttt tccacgtcgc tttcacgcgc ggtcccacgt ccgtagagta accgaccccg
    32881 gtttcgatag tgttttctgt tcgcggcgaa accacgcccg tcaatggtaa tactttcgtt
    32941 tcgcgcgctt tatcaaattg tggagccacc accaccgagt taaaccagcc ggtagcagaa
    33001 agtcggcggt tcagttctgc cagatctttc gattcgtact catcgccctc tttaaacggc
    33061 accagatttt gcaggtattc atcgcggatt tgtgatcctt caaaggtcac atgcccaaag
    33121 cggtaacgtt cgccactgtt ataatcaata tcccagaagg ctttatgcag gccgagcgca
    33181 atgcccagct gcgctttggt aaattcgcta tcgaaataac ctttacgcaa cgcaatgctg
    33241 gttaaggact ttttgaaatt ttcataatcg ccctggttca gtaccgtgcc aatagccggg
    33301 cgagtatcga gcaatttcaa atagtcttta tcggtccgcg cgcccccgcg caataccaca
    33361 tcggtgccgc caattaacac cggcacgcct ggcgtgactt tggcgatcaa tacctgccgc
    33421 cctttctttg gcggtggacg gagatcaaat tcaatggtcg gctggtaata acccagcgct
    33481 ttcagacctt cgcggatagc atcatcgacg cgtgcgcgaa agcgacggtc tggcgtcact
    33541 tcatcacttt caatcgtaga aagctgcgca cgaacgttct tttccagctg tcccgataac
    33601 ccctcgacct gtagacggac gttcgcggcg acggcagatc cgcttaagca gagtaagctt
    33661 acacagcata actgtcggat atagcgcaca ttttctcctg aatatccttt tttcctgccc
    33721 ctggaacgcc gttaaacggc ttaacaaaaa tccagtaata tggattaaaa agcagactaa
    33781 accccaaata tttcttatgt tttactttag acctattcac ggtggttatt gtgtgcaaat
    33841 acgcctcttg ttacaacctt aaccccaatg accgattttc gggagagcga caccatgagt
    33901 ttatttgata aaaagcatct ggtttccccc gccgatgccc tgcctggacg taacaccccg
    33961 atgcccgtag ccacgctgca tgcggttaac ggtcactcaa tgaccaatgt acctgacgga
    34021 atggagattg ccatttttgc gatgggttgt ttctggggtg tggagcgtct gttctggcag
    34081 ttacacggcg tttacagcac cgccgcaggt tataccggcg gctatacgcc aaatccgact
    34141 tatcgggaag tgtgctccgg tgatactggt catgccgaag cggtacgcat tgtttacgat
    34201 ccttccgtca tcagctatga gcagttgcta caggtatttt gggagaatca cgatcccgcc
    34261 cagggaatgc gtcagggcaa tgaccacggc acgcagtatc gttcagcgat ttatccgctg
    34321 accccagaac aggatgccgc agctcgcgcc agtctggaac gttttcaggc ggcgatgctt
    34381 gccgccgatg acgatcgtca catcaccacg gaaatcgcta acgccacacc gttttattat
    34441 gccgaagatg accaccagca atatctgcat aaaaacccgt acggttactg tggaattggc
    34501 ggaattggcg tctgtctgcc accggaagca tagcgttacg ggtacaaatg tagattgttg
    34561 ataaagtgcg ttttgtttat gccggatgcg gcgtaaacgc cttgccaggc ctacaaaatc
    34621 gtccaaattc aatatattgc aaggactgcg taggcctgat cggcatagcg catcaggcag
    34681 ttttgcattt ttcagctgtc tgaattttaa ccctctggcg gctttacagt accttacgct
    34741 atactagcca ctgaaaatgc cggttcactt tcttcgaatc ggctttcaat gtgtatttca
    34801 cacaaattaa tcaacttccc cttccgagga tctggcctga aaggtcggat aagatatgtt
    34861 aaacagtatt ttagtcatac tctgcttgat cgctgtaagt gcgttcttct cgatgtctga
    34921 gatctcactt gccgcctcac gcaaaatcaa acttaaactg ctggctgatg aaggcaatat
    34981 aaatgcccaa cgcgttctga atatgcagga aaatcccggc atgttcttta ccgtggtcca
    35041 aatcggtctg aacgcagtgg cgattctcgg cggtatcgtc ggtgatgcgg cattttctcc
    35101 agcttttcac agcctgttct cccgctatat gtcggcagag ctctctgagc aactgagctt
    35161 tattctctct ttctcgttag tgactggcat gtttatcctg tttgcggatt taaccccgaa
    35221 acgcatcggt atgattgcgc cagaagctgt ggctttgcgt atcatcaacc cgatgcgctt
    35281 ctgcctgtac gtttgcaccc cgctggtgtg gttcttcaac ggcctggcga acataatctt
    35341 ccgtattttc aaactgccaa tggtacgtaa agatgacatc acttctgatg acatctacgc
    35401 ggtagtggaa gccggtgcgc tggcgggcgt gttacgtaaa caggaacacg agctgattga
    35461 aaacgtcttt gagctggaat cccgtaccgt tccgtcttca atgacaccgc gtgaaaacgt
    35521 gatttggttt gatctccacg aagatgagca aagtctgaaa aataaggtgg cggaacatcc
    35581 gcactctaag ttcctcgtct gtaatgaaga tattgaccac atcatcggct atgtcgattc
    35641 taaagacctg ctgaaccgcg tgctggctaa ccaaagcctg gcactgaaca gcggcgtaca
    35701 aattcgcaac acgctgattg tgccggatac gttaaccctt tcagaggcgt tggaaagttt
    35761 taaaaccgca ggagaagact tcgcggtgat catgaacgag tacgcgctgg tagtggggat
    35821 catcaccctc aacgacgtga tgaccacgct gatgggcgat ctggtcggtc agggactgga
    35881 agagcagatt gtcgcccgtg atgagaactc atggctgatt gacggcggca ctccgattga
    35941 cgacgtcatg cgcgtgctgg atattgacga gttcccgcag tcgggcaact acgaaaccat
    36001 cggcggcttt atgatgttta tgctgcgtaa gatcccgaaa cgcaccgatt cggtgaaatt
    36061 cgccggctac aaatttgaag tggtggatat cgataactac cgcatcgacc agctgctggt
    36121 gacccggatc gacagcaagg ccacagccct ttcgccaaaa ctgcctgacg ctaaagataa
    36181 agaagaaagc gtcgcgtaac ccccataaac atcaacggct cctgaatcag gagccgtctt
    36241 attactgcat agcacttttt actgcatagc actttggtta agccatctct gtttgcagac
    36301 gcataacctg acggttaact tcggacatca ctgacaaatg cagcttatcc ttcacttttg
    36361 ggataagaat cttaccctta tcgaattcaa aagcgccaac gtccttgatg tataatcgtc
    36421 cacggaacag gatctttacg tacttcgcca cctgaagtgg gttgtaccgt tggaaaattt
    36481 tcattgttat ctcctgctga gtattacgcc tgtgcggtgc cacaatcggc ccaactatta
    36541 tgaggcgcaa attttaatgc ttagtgacta tagactatcc gggcaatgtt tccaccgcgt
    36601 ataacttttt ttacctaaag gttacaatta ttcagaatta tctttttacc gaagcgcggt
    36661 cttcagtata agcattcatt tttcatatga tttgtgcgct tgaccgcaaa ctggcatcac
    36721 acttgcggga aattcgataa atagcacata tgattaaaac tcagacccaa gtggtcggat
    36781 cacctgcata tcataagaag gaaacaccat gaccctacgc aagattctgg cactcacctg
    36841 cctgctgttg ccgatgatgg cttccgcaca tcagttcgaa accggtcagc gagtgccgcc
    36901 gattggcatt accgatcggg gcgagttggt gcttgataaa gatcagttta gctacaaaac
    36961 ctggaacagc gcgcagttag tgggaaaagt gcgagtactg caacatattg ctggtcgcac
    37021 ctctgcaaaa gagaaaaacg cgacgctgat tgaagcgatt aaatcagcga agttaccgca
    37081 cgatcgttac cagaccacca ccattgttaa caccgacgac gcgattccgg gttccggcat
    37141 gtttgtgcgc agcagtctgg agagtaataa aaagctttat ccctggtcgc agtttattgt
    37201 cgatagcaat ggcgtcgcgc gcggtgcctg gcagctggat gaagaaagtt ccgctgtagt
    37261 ggtgctggat aaagacggtc gcgtgcaatg ggccaaagac ggggcgctca ctcaggaaga
    37321 ggtgcagcaa gtgatggacc tgctgcaaaa gttgctcaaa taatggcaat aacataaatt
    37381 ctgcaaaaat aattttcccc tatcgttatc tatgggatag gggaattaag tctctgagtt
    37441 cttacgccga actaatactg gtaacgctag ttgctctttt ttaaacgcct caatggtcga
    37501 tttatatttc tcgcaaatct tttctttggt aatatcacta tctgcggcca gattcgcact
    37561 tactatcgca tccacttgtt cggctaattc gacttgttgg caacccgcat taaccagaaa
    37621 attataaata agagagtaat cttcatcctc caagacaaca gaggaaccat tacgaaaacg
    37681 ttcatcccct ccatagattt taagcgcaaa gcccaaagat tctatgacta tggcaaaatt
    37741 atcatcaatt attttttcgt aaatatacca tccatcgcac tgtttaattc cacctcctgg
    37801 cccttgtagt tcaaatttac cccgttttaa tttgcttact atatcatcca ataaagcata
    37861 acctaaagac acgtcaccaa tcgcctggtg aatataacct ttaatccttt tcggtatgtc
    37921 agactgtttt ttctgcagaa tatcccaggc atacaatcgt ctgttttgtt ctaacggaaa
    37981 ttcacggttc ttgaaatcca tgaatgagtt atactcattt attgttatgg ggatttcttc
    38041 gtattcaaat ccactgcctc gcaatgtcat tgtatattcg gcaccgttat ctttaaggct
    38101 gaaactgata agaaacttat ccatagcgtt aacagtttca ttttttgatg aaagcacctg
    38161 ataactactt ttatcaatct gagttgcgca acgaaagaac tccctcaaag ttaacaggct
    38221 atcataattc gaaatccaat cttttttctt aaatgcatga tggatgttgt taaaaaaatt
    38281 gtttgaatgt gtttttgtat acgaagactt tacaaataac tccaccgcac caataaaatt
    38341 attgtgtctg atcctgggta acatatgaca accttatttt atttggttac tttatatcgc
    38401 cattaaatat ctcactatag aagtaaacta tcaaaaaacg ctcattatta aaatgtaact
    38461 tattagcaat tgaatttttg cactttaaat acaatctctt tttatttata actttttagg
    38521 ttgggagaaa tcaggatggc agaatcagga aatacactgt ttctgccatc tgaatttagt
    38581 aaatagacac tctgaacccc ggattcagga acgactcgcg cggagtgtaa tccagcggtt
    38641 taccctgcca gtcgtgaacg tgcgctccgg cagctgcagc cacagcatgt ccagcggcgg
    38701 tgtcccaaat attcgttggt ccgaaacgcg ggtacagctg cgcctgtcct tccgccacca
    38761 ggcagaactt cagcgaagag ccgatggacg tggtctgatg ttcgccaagc tgttgcagat
    38821 actctttcag ctccgcatcc gcatgagagc ggctgatcac caccagcggc gggcgcgcat
    38881 cgcggacctg aatctgcttg cgcacgccgc actcttcttt ccaggccttg ccttctgccg
    38941 cgctgtacat tacgttcatt accggcgcat acaccacacc taaaatcggt ttgccatggt
    39001 caatgagcgc aatgttaacg gtgaattcgc cattacgttc aataaactct ttcgtaccat
    39061 ccagcgggtc taccagccag taacgctgcc agtgctgacg gacttcccaa ccgggaggat
    39121 cttcttcaga aaggaccgga atatccggtg tcagcgtacg taaaccgtcc atgataacgg
    39181 tgtgagcggc aatatccgct gccgttaccg gagaattgtc cgctttgctg acgacgtcca
    39241 tcggtttcgc cccgtcgtag acctgcataa tggcatcgcc tgcattccgt gcaagctggc
    39301 atacttgatc taacatttct ccacctcgtc tctgtgagcg gtgttaactt attgttttac
    39361 ttatacccta tcattaatga atgcgccaac tgtgatagtg tcatcatttt caaagcgtaa
    39421 aattgtggca ttcttcactg ttctataagt aagacgttta ttctcccttt tctttcgtat
    39481 tcccgatgat aaaaggatgt ccctgatgat taagtttagc gcaacgctcc tggccacgct
    39541 gattgccgcc agtgtgaatg cagcgacggt cgatctgcgt atcatggaaa ccactgatct
    39601 gcatagcaac atgatggatt tcgattatta caaagacacc gccacggaaa aattcggact
    39661 ggtacgtacg gcaagcctga ttaacaatgc ccgcaatgaa gtgaaaaaca gcgtactggt
    39721 cgataacggc gatttgattc aggggagtcc gctggccgat tacatgtcgg cgaaaggatt
    39781 aaaagcaggt gatattcacc cggtctataa ggcattaaat acgctggact ataccgtcgg
    39841 aacgcttggc aaccacgagt ttaactacgg tctggattac ctgaaaaatg cgctggcagg
    39901 agcgaaattc ccttatgtaa atgccaacgt cattgacgcc agaaccaaac agccaatgtt
    39961 tacaccgtat ttaattaaag acaccgaagt ggtcgataaa gacggaaaaa aacagacgct
    40021 gaagattggc tatattggcg tcgtgccgcc gcaaatcatg ggctgggata aagctaattt
    40081 atccggaaaa gtgacggtga atgatattac cgaaaccgtg cgcaaatacg agcctgaaat
    40141 gcgcgagaaa ggtgccgatg tcgttgtcgt tctggcgcat tccgggctgt ctgccgatcc
    40201 gtataaagta atggcggaaa actcagttta ttacctcagt gaaattccgg gcgttaacgc
    40261 cattatgttt ggccatgctc acgccgtttt cccgggtaaa gattttgctg atatcgaagg
    40321 ggctgatatc accaaaggca cgctgaatgg tgttccggcg gtaatgccgg gcatgtgggg
    40381 cgatcatctt ggggtggtcg acttacaact cagtaatgac agcggtaaat ggcaggtgac
    40441 gcaggcgaaa gcggaagcac ggccgattta cgacatcgcc aataaaaaat ccctcgcggc
    40501 ggaagacagc aagctggtag aaacactcaa agccgatcac gatgccacac gccagttcgt
    40561 cagcaagcca atcggtaaat ctgccgacaa tatgtatagc tatctggcgc tggtgcagga
    40621 cgatccgacc gtgcaagtag tgaacaacgc gcaaaaagcg tatgtcgagc attacattca
    40681 gggcgatccg gatctggcaa aactgccggt gctttcagct gccgcaccgt ttaaagtcgg
    40741 tggtcgcaaa aatgacccgg caagctatgt ggaggtggaa aaaggccagt tgaccttccg
    40801 taatgccgcc gatctttatc tctatcccaa tacgctgatt gtggtgaaag ccagcggtaa
    40861 agaggtgaaa gagtggctgg agtgctccgc cggacagttt aaccagattg atcccaacag
    40921 cacgaaacca cagtcactca tcaactggga tggtttccgc acttataact ttgatgttat
    40981 tgatggtgtg aattatcaga ttgatgttac ccagcccgcc cgttatgacg gcgagtgcca
    41041 gatgattaat gccaatgcgg aaaggattaa gaacctgacc tttaatggca agccgattga
    41101 tccgaacgct atgttcctcg ttgccaccaa taactatcgc gcttacggcg gcaaatttgc
    41161 cgggacgggc gacagccata tcgcttttgc ttcaccggat gagaaccgct cggtgctggc
    41221 agcgtggatt gctgatgagt cgaaacgtgc gggggaaatt cacccggcgg cagataacaa
    41281 ctggcgttta gcaccgatag ctgccgataa gaaactggat atccgtttcg aaacttcccc
    41341 gtcagataaa gccgcagcgt ttattaaaga gaaagggcag tatccgatga ataaagtcgc
    41401 gaccgatgat atcgggtttg cgatttatca ggtggatttg agtaagtaaa acacttcttt
    41461 ttcggcctat aaatcatcaa tcgcatccgg catttattgg cggatgcgat gctggcgcat
    41521 cttatccgcc ctacaagtca tacaccgtag gccgcgcatc cggcagttat gccgcacgtt
    41581 catcccgcac cgccaacacc tcgggcaaat tcaactcaat ccagtccgcc agtgcagcaa
    41641 ccttttcgct cacctgctcg cccagcggcg tgaggctgta ttcaacatgc ggcggcacca
    41701 ccggatacgc gatacggtta agaaaaccat cctgttctaa cgcctgtaac gactgcgcaa
    41761 gcatcttttc gctcaccccg ccgattttgc gccgcaggtc gctaaagcga tgagtacctt
    41821 cgcgtagcgc caccagaatc aacacccccc aacggctggt gacgtgtttc aacacctcgc
    41881 gcgacgggca ctgttccgca aagagattac cctctttcag ttgttgcgac aggctaacct
    41941 gactcatttc atacttacct ttttgtgcgt acttactaaa agtaagttta ggtgttagcg
    42001 tatttaaaca caagacaaaa cgatggagac ttcccatgat cgctattact ggtgccactg
    42061 gccaacttgg tcagtatgtt attgaatcct tgatgaaaac ggttcctgcc agccaaatag
    42121 tggctatcgt tcgtaatccg gcaaaagccc aggcactgac agcacaaggc attaccgtgc
    42181 gtcaggctga ctacggcgat gaagccgcac tgacatctgc acttcaggga gtggaaaaac
    42241 tactgctgat ctcttccagc gaagtgggtc aacgtgcccc gcagcatcgt aatgttatta
    42301 atgccgcaaa gacggctggc gtgaaattta tcgcttatac cagcctgctt cacgcggata
    42361 aatctccgct cggcctcgcc gatgagcaca tcgagacgga gaaaatgctg gctgattctg
    42421 gcatcgttta caccctactg cgcaacggct ggtacaccga aaactacctc gccagcgccc
    42481 cggcagcact ggaacacggc gtatttatcg gtgcggcggg cgatggcaaa atcgcctcag
    42541 caacgcgggc agattatgcg gcagctgcgg cacgcgtgat tagcgaagcc ggtcacgaag
    42601 gcaaggttta cgaactggcg ggcgatagtg cctggacgtt gacacagtta gcggcagagc
    42661 tgaccaaaca gagcggcaaa caggttacct atcaaaatct gagcgaagcc gatttcgccg
    42721 cggcactgaa aagcgtcgga ctgcccgacg gactggcgga tatgctggcg gattctgacg
    42781 ttggcgcatc gaaaggcggt ctgtttgatg acagcaaaac acttagcaaa ttgattggcc
    42841 acccaacgac aacgttagcc gaaagcgtaa gccatctttt taatgttaat aactagttaa
    42901 ttaaagtggc atcctcccgc atcctctctg ataatgacgg gatgccggga gcaatcatgt
    42961 ctgcttcctg aactttcttc tgacagatca atggatgcca gtaatgatta gcggcgtgct
    43021 gtacgccctg ttagcagggt tgatgtgggg gcttattttt gtcgggccgt tgatcgtgcc
    43081 ggaatacccg gcgatgttgc agtcgatggg gcgttatctg gcgttagggt taattgcgct
    43141 gcccattgcc tggctgggac gcgtgcgtct gcgtcagttg gcgcgtcgcg actggcttac
    43201 cgccttgatg ctcactatga tgggcaacct catctattac ttctgccttg ccagtgccat
    43261 tcaacgtact ggtgcgcctg tttccacgat gattatcggc accctgccgg tggtgattcc
    43321 cgtcttcgcc aatctgcttt atagccagcg cgacggcaaa ctcgcgtggg gaaaactcgc
    43381 cccggcactg atttgtattg gcatcggcct ggcgtgtgtg aatattgctg agttaaacca
    43441 cggactcccc aattttgact gggcacgtta tacctctggc atcgtgctgg cgttagtttc
    43501 cgtggtctgc tgggcgtggt atgccctgcg caacgcccgc tggctgcggg aaaatcccga
    43561 caaacatccg atgatgtggg caacagcgca ggcgctggtc acactgccgg tttcacttat
    43621 cggctatctc gtcgcttgtt actggctgaa tacgcaaacg ccggacttct ccctaccctt
    43681 tggcccccgt ccgctggtgt ttattagtct gatgattgcg atagccgtgc tttgctcatg
    43741 ggttggcgca ctctgctgga acgtcgccag ccagcgatta ccgacagtga ttctcgggcc
    43801 gctgattgtt ttcgaaacgc tggcaggttt gctgtacacc tttttactcc gccagcaaat
    43861 gccgccgcta atgacgctga gcggtatcgc gctgttagtg attggcgtgg tcatcgcggt
    43921 tagagcaaaa ccagaaaaac ctttaactga atctgtctca gaaagttgac acgctggcag
    43981 tgagttaaat aagcctctgc tacgtaaggg ttatagcttt tgccttaaag atgcatttaa
    44041 aatacatctt atcttattaa gaatgaggta tcagctatgg cttatcgcga ccaaccttta
    44101 ggtgaactgg cgctctctat tcctcgcgct tcggctctgt ttcgtaaata tgatatggat
    44161 tactgctgcg gcggtaagca aacgctggcg cgtgcggcgg cacgtaaaga actggatgtt
    44221 gaggtcattg aagctgagct ggcaaagctt gctgaacaac cgattgagaa agactggcgt
    44281 agcgccccgc tggcagaaat catcgaccat atcatcgtgc gctaccacgc tcgtcaccgc
    44341 gagcaactgc cggagctgat tctgcaagcg actaaagtcg agcgcgttca cgccgacaaa
    44401 ccgagcgtgc caaaagggct gacaaaatac ctgaccatgc tgcatgaaga gctttccagc
    44461 cacatgatga aagaagagca gatcctcttc ccgatgatca aacaaggcat gggcagccag
    44521 gcaatggggc caatcagcgt aatggaaagc gagcacgatg aagcgggtga actgctggaa
    44581 gtgatcaaac acaataccaa taacgtcaca ccgccgccgg aagcctgcac cacctggaaa
    44641 gcgatgtata acggcattaa tgaactgatt gatgacctga tggatcacat cagcctggaa
    44701 aacaatgtac tgttcccaag ggcgctggcg ggtgagtgat ctaaaagctg gatggcattg
    44761 cgccatccag catgataatg cggttatttc cgcagttcag cagcccgctt cttaccaata
    44821 aacaaccagc ccagccccag cgcgataaac cacagcgggg taaccagcag cgcctggcga
    44881 gtgtcatctt ccagtgtcag caacacgacc acgaacacaa agaacgccat acatacccag
    44941 cacatcagct tgccgagcgg catcttgtag atcgacttct catgtagatg aggacgctgt
    45001 ttgcgataca caaggtacga gcaaaggata atcgtccaga cgaacataaa cagaatcgcg
    45061 gaaacggttg taatcatcgt gaacgcgcca atcacgctag gattcacata caacatcacc
    45121 acgccgccga gcagacagat acacgagaaa gtcaaccctt tcgcgggtac tgcgcgctta
    45181 gaaagtttag cgaacgcttt cggtgccaca ccttcctgcg ccagaccaaa cagcatacgg
    45241 ctggtagaga agacgccact gttagcggaa gacgccgcag aggtcagcac cacaaagttg
    45301 atcacgctgg cagccgcagg caaacctacc aacacaaaca gttcaacaaa cgggcttttc
    45361 tccgggacta ccgaactcca cggcgtcacg gacataataa caatcagcgc gaagacgtag
    45421 aacataatga tacggatcgg aatggagtta atcgcgcgtg gcagtgattt ctctggatct
    45481 ttggtttccg cagctgttgt acctaccagc tcaatcccca cgaaagcgaa aaccgctatc
    45541 tggaatccgg caaagaagcc acttaaacct ttcgggaacc agccgccgtc attccacaaa
    45601 tgcgcgaatg acgcttccac accagtcggt gactgaaagt gcatcgccac catgaccagg
    45661 ccgacgacaa tcagcgacac aatggcgacg attttgatca tcgcaaacca gaactccatc
    45721 tcaccgaaca ttttcacagt ggcgaggtta agcgtcagca gcagcactat caccgccagc
    45781 gaggcgaccc agtcggagag atcggggaac cagaactgag cataagccgt gatcgccacc
    45841 acgtctgcca taccggttac aacccagcag aaccagtaag tccagccggt gaaatatcct
    45901 gcccacggcc cgagtaaatc ggaagcgaag tcactaaaag atttgtattc cagattcgaa
    45961 agcagcaatt cccccattgc ccgcatcacg aaaaagagca taaaaccaat gatcatataa
    46021 acgaaaatga tcgacggccc ggcaaggcta atcgttttgc cagaccccat aaacaacccc
    46081 gtaccaatgg caccaccaat ggcaataagc tgaatatgtc ggtttgtgag attgcgccgt
    46141 agcgactgtt cagccggagc ctgatcatcg gcaacgactt ttacctgatc taccatgttt
    46201 ttttcttcct gtacctgtct gtgttgttca ggctctacgg cctttagtca gtctatgata
    46261 cgacgaatct gtatcgggac tcatcgatat taggtaacaa tacgcgggat gaatactaag
    46321 atttagatct aatgttaatt ttatgtttaa agtgagtgtc atatcacggt gataacgcga
    46381 aacagctcac aaaaatacac gcttacgggg tctttgcaac acaaaatagc gccgtttggc
    46441 aaactgaagg gtttattgct gaatgcctgc tcccctctgg ttcgagggga acagaatgcg
    46501 ctgcttagag gatttccagc agttcgactt caaacaccag ggtgctgaac ggagggatgg
    46561 atgcgcctgc gccgcgctcg ccatatgcca gttcctgcgg gatagtcagt tcccatttag
    46621 aacctaccgg catcagagtc agtgcttcaa tccagccagg gatcacgcca ttaaccggaa
    46681 attcagcggg ttcaccacga gcaacggagc tgtcaaacac ggtgccgtcg atcagtttac
    46741 cggtgtaatg aacacgaacg cggtcggtac gtgccggaat tgcgccttca ccctggttga
    46801 tcacgcggaa ttgcaggcca gattcggtgc tattcacacc ttcttttttg gcgttttctt
    46861 ccaggtattt cacaccttca gcagccatcg cctggaaacg ctgacgacga acggcatcgg
    46921 cgcgctcgtg gatttcacgc agcgcgcgat gcaccacatc aaccggaaca gccggatgtt
    46981 tgccttccag cgcatcggca atacctgcaa ccagtgcttc tggcagcagc ccttccaggc
    47041 cagattcact cagttgttgc cctacctgca aaccaatgcc gtagcttgct tgcgcttcga
    47101 tggtgtcaaa agttggggtg gtcatcgtat ttcctttctt agggtataaa gtagcgggca
    47161 gcatatcagc cacacttctt tgggtaaaac tttgtcgcgg gaatgatgac aaatcccagg
    47221 gaaaaagaaa caatagcaat atcccgtatg aatataataa aggcgggttt accacggaca
    47281 tcatagcgtt agcttattca taggctatga ttgaggaaca agacgcggag caggaggaaa
    47341 ccatgcccgg gcgctttgaa ctaaaaccaa ccctggagaa agtctggcac gcaccggaca
    47401 attttcgctt catggacccg ctgccgccga tgcatcgtcg ggggatcatt attgccgcca
    47461 ttgtattggt ggtcggattt ctgcttccat ctgatgatac gcccaacgcg ccggtggtga
    47521 cgcgtgaagc gcagttagat attcagtcgc aatcacaacc gccgacagaa gaacagctgc
    47581 gggcgcaatt agtcacccca caaaattatc ctgatcaggt ggctccggtc gctccagaac
    47641 ctatacagga aggccagcca gaagaacagc cgaaaactac ccaaacgcaa ccattccagc
    47701 cagatagcgg aattgataat caatggcgct cttaccgtgt cgaacccggt aaaacgatgg
    47761 cgcagctgtt ccgcgaccac ggactgcccg ctaccgatgt ctatgcaatg gcgcaggtag
    47821 aaggcgcggg taagccgctg agtaatttgc aaaacgggca gatggtgaaa atccgccaga
    47881 acgccagcgg cgtggtgacg ggcttaacca ttgatacggg gaataatcaa caggtcctgt
    47941 tcacccgcca gccggatggc agttttattc gtgcgcggta gatgaagggt caaccaaaag
    48001 ccagctaagg attgtctctt tgatgacctt acgtctggct tccagtgcct ggggtgaaac
    48061 catactgatg tgttgacatc ctccaagccc tgaaggacgt ggatccctgc tacgttcagg
    48121 ctgtcgcctg aatcatttcg gtgggttcct gcttcaacgg gcggcctgac tgcaccatct
    48181 ccccacaggc aagaacggcg tgccccgccg ctaaaatgtt acgagcgcca tttacatcag
    48241 cgttcgctgt atatccacat gcctggcatc tgaatttact ttgtgacagg cgattttctt
    48301 tcgctgtatg accacagcac gcgcaacgct ggcttgtgta cgctggcgga acagcaagca
    48361 cctgaccgcc acgccagagc tgcttgtact caagctggcg gcgcatttca taccagccct
    48421 gatccagtat cgaacggttt aaacctgatt ttgcccggac attgcgcccc ggctggctga
    48481 ccgtacccgc tgctgacttt gacatgttgc tgaccttcaa atcctcaatg actatcattg
    48541 cgtggttttt gctgacggtc gttgtgactt tgtgaaggta gtccctgcgg atatttgcga
    48601 tacaggaatg cagtcgctgt attttgcgtt tctgcttctg ccagttgttg ctgaatttga
    48661 ccttgcggct taactgtcgc tgaagtctcg ccagcgtctt ctggtttttc tgaaaactgt
    48721 ttacaggtcc aaagactgtg ccatctgaca gcgtggcgag tttagccacg ccagcatcca
    48781 gtccgaccat taatgctgaa gggtgaaccg gagttgatac ttcattttca gtctgaatac
    48841 tgatgtacca cttaccgcag gactggctgg cagtgacatt tttcacaaca cccgtgacct
    48901 gacggctatt ccggtagcgc atccagccaa gtttcggcag aaaaatacgg ctgttttcct
    48961 gatcgagctt aacgccctgc gggtagcgga atgcatcatt ctgtccccgc tttttgaatc
    49021 ggggaaaagc agcccgcttc cggaagaagt ttttgtaagc ccgctcaagg tctttcagtg
    49081 actgttgcaa tggctgtgac ggggcatctt taagccattg cgtttcagtg gcatttttcc
    49141 actcaaccag ccaggaagcc attttcccgt aagggatgta tttattaccg gcctcatgat
    49201 tctcattctg aagcgccaga gcacgattga aaacgaaacg acatgcgcct gcgaagcgcc
    49261 tcatttcacg ctcctgttga ccaccgggtc ttaactggaa tttaaatgcc tgtagtcgtt
    49321 tcacaggcca aatgatactt tggtctgtga aaaaagaaac cgatatccgg cgtggaaggc
    49381 attgtgtttt cctgatgcat gttcacctgg tctttgtcac cagataccga cgccagattt
    49441 ttgaccatga cgcgacagaa aaactacgca cttacttttc aaaggtatgt gctgattttg
    49501 aagctgaact ggttgaaatg gatggcgaac cagatcacgt ccatttgtta attaactatc
    49561 ctcccaaact ggcgatatcc agtctggtaa acagcctcaa aggcgtatcg ggtaggttac
    49621 tgcgacgaga tcgaccagat attgcagtca ggtattacta caaaggcgtt ttgtggagtc
    49681 ctggctattt tgccagtagc tgcggaggtg cgccaatatc cgccatccgt caatacattg
    49741 aacaacagca aacacctggt taggtggaaa accgcgccgt atatccccgc cctgaaggac
    49801 ggggttttac ggcgcaccgg ataaaccagg ccaagcgtat gctggttgat caaataatat
    49861 cccccaagcg cagcaatatt aatattcacc tgcaatgggt cgatatctgg cttaaaaata
    49921 ttgtatttct tcccttcatc caataaggat tccattaact gcaagtgcgc atggttgatt
    49981 tctaagagac gctgtgattt tgcataatgc acacctttac tttgattttc actatgcaca
    50041 atttttaaaa accaggggtt agcaatataa taatcccatg taaaatcaat aattttctca
    50101 atggcttttt gtggctggtt aacctgctga ttaacgaata caccgatacc gacagcgtag
    50161 aataattaca taccgcacaa caagaatgta cggtgtaagg cgtggaagtc attttctccc
    50221 cgagtgcatc gttatatcca taatgagcga tttttgatat taatatcttt caccatccgc
    50281 tattttcacc acttcagaaa acaaacttat ttttattaaa atcgattttt ttctgtgaac
    50341 attcagctgc gatatttata aaaacattaa acaggtcaca ttacaatgac attaccaacc
    50401 actatttatt catttcctgc atatttatct cgtttttcgt ccaccgacaa accagtaaaa
    50461 ttaaaatttc atcaatacgc acgcgctaca ttactttcaa atcgtggaag agatcataat
    50521 tgcgatggca gacgtactgt tgagatccac aaactagatc tctcagactg gcaagcattc
    50581 aataagttag caacgaagtg taatgcttac gatggagtaa caatgaatgg tgataattct
    50641 tttggatgga accatgaagc tacattagat aatattcacg cccagaaata caataaagct
    50701 tacgcgggtg cgaggttaac cgcagaactg aaatatttat tgcaagatgt cgaatccttc
    50761 gaacccaaca gtaaatacac aatacacgaa gtggttttgg gacctggtta tggtactcca
    50821 gactataccg gccaaacgat aggatacgtt gtaacccttc ctgcccaaat gcctaattgc
    50881 tggtcatctg aactaccaac aatcgatctt tatatcgacc aacttcgcac tgttacaggc
    50941 gtttctaatg ccctcggatt catcattgct gcattgctaa acgcgtattc tgatttaccc
    51001 catgatttga aaattggtct gcgttcctta tcaagttcag ccgcaatcta ctcgggtttg
    51061 ggtttcgagc gagtgccgca ggagcgagac atcagttgcg ctcgtatgta tctaacgcca
    51121 gcaaaccatc ctgacttatg gacacaagag aatggtgaat ggatctactt gcgaaactaa
    51181 tacctgacga aaaatagaaa gcaaaacgcc gaccagtggt cggcgttttt acgtctcgtt
    51241 gaataacgaa ttattcagct actacgttta cgatcacttt cgcgaatact tcgctgtgaa
    51301 cctggaagct cacttcgtgt tcgccagtgg tacgcagaac gccgttcggc agacgaactt
    51361 cgctcttagc cacttcaacg ccagctgcag ttacagcgtc agcgatgtcg cgagtaccga
    51421 tggaaccgaa cagtttacct tcgtcgccag ctttagacgc gatggtaaca gtttccagtg
    51481 cattgatttt ctcagcgcga gcattagcag ctgccagaac ttcagccagt ttagcttcca
    51541 gttcagcgcg acgtgcttcg aagaattcaa tgtttttctt ggtagctgga acagctttac
    51601 cctgtggtac caggaagtta cgagcatagc ccgctttaac gtttacctga tcacccaggc
    51661 tacccaggtt tgctacttta tcaagcagaa taacttgcat taccttatcc tctcaaagtc
    51721 gtattaatgg accgtgaccg attactgatg gcgatcagtg tacggcagca gggacaggta
    51781 gcgagcgcgt ttgatagcgc gagccagctg acgctggtat tttgcacggg taccggtgat
    51841 acggcttggg acaatcttac cgctttcggt gatgtagttt ttcagcgtag cgatatcttt
    51901 atagtcgatc tcttgaacgc cttccgcggt gaaacggcag aacttgcgac gacggaaata
    51961 acgtgccata tggctagtct ccagaatcta tcaattcaat ctgctcggca tgcaaaacca
    52021 ttttgctcag tccgttcttt gccttgtggc atgaaatgaa cccctgaacg gttatgcgac
    52081 tgccgaccgt tatactgtga gtaatggcct ggttttcgtg tccgctaaca ataacgggca
    52141 tttgacacca tgcctgccgg tgaaagccgg cttcctcctg cacagaacga tgctcaagca
    52201 cgaactggca gtgaggaatt cctgatggac tgacctttcg aaggggagtc ctgcacacgg
    52261 tgccggacaa caccagacgg ttggtcatca gaaattactc ttcagaatcc ccagcttcag
    52321 catcatcagc ggtttcgttt gcgaaatcat cgcgacgctc acggcgctcg tctttcgctt
    52381 taaccatcgg agatgcttcg gtaacagcgt gcttggtacg cataaccatg ctgcggataa
    52441 cggcatcgtt gaagcggaag gtagtttcca gctcatcgat cacttcctgc ggagcttcaa
    52501 cgttcatcag aacgtagtga gctttgtgca gtttgttgat cgggtaagcc agctgacggc
    52561 ggccccagtc ttccagacgg tggatcttgc cttctgcacc agtgatggca gcagtgtagc
    52621 gctcgatcat gcccggaacc tgttcgctct ggtcaggatg gaccataaaa acgatttcgt
    52681 aatgacgcat cgaattgctc cttacggatt attcagcctc ctgtctgggt cagccgaatc
    52741 ccggggaggc aaggaacgtg ttaaaggtcg gctgaaaaat gacgcgttat agtactgaca
    52801 taccccgcta aactcaaggc cagccgacaa ataaatctca tctttcacca aagtggacta
    52861 acacgctgat ttgaagttca tcacacttca tcatggtgtt ttttcgaaca actgcatcag
    52921 aaatggtcac catcaacttc tttatcattg attaaaatta aatcagaaga tcatcacaga
    52981 ggagagcaca caaagtcagc cgtcgaggag gtatctatgt tcagtcgagt tttagccctt
    53041 ctggctgtgc ttttgctaag tgcaaataca tgggcagcca ttgaaattaa taaccaccag
    53101 gccagaaata tggacgatgt gcaaagctta ggcgtgattt atatcaatca taatttcgcc
    53161 actgaaagcg aagcacgtca ggcattaaat gaagaaacag atgcgcaagg cgcaacgtac
    53221 taccacgtaa ttctgatgcg ggaaccgggg agtaacggca atatgcacgc cagcgcggat
    53281 atttatcgct agcaccaggt ataaccaacg aaacattgcc atagtttgct ttgccccctt
    53341 cgcagggggc tttttttgaa aaacggctgc ggaattagac cagttatctc ccgaggaagg
    53401 aaatttccgc agcgcgtgtt ctacttctgc ccgtaataag cgttggggcc gtgcttacgc
    53461 atgaagtgtt tattcatcag gaagctgtcg atgtgattga gttgtggatt aatactgcgc
    53521 gcaatccacg ccattttcgc cacttcttcc atcactaccg cgttatgcac cgcgtcgtgg
    53581 gcatctttcc cccaggcgaa cggtccgtgc tgatacacca caattcccgg cgtatgcagc
    53641 ggctcggcgt tgcccagcgt ttcgataatc actttgccag tgttcagttc atactcaccc
    53701 tgcacctctt cttcgcttaa tccacgcgta cacgggatat caccaaagaa gtagtcagcg
    53761 tgcgtggtgc ctaacgccgg gatcgccagc cccgcctgcg cccatgcggt agcatgagtg
    53821 gagtgggtat ggacaatgcc gccaagcgac ggataacgac ggtagagttc gagatgcgtc
    53881 gcggtgtcgg aagacggacg atattcccct tccaccacct taccgctcat atcgaccacc
    53941 accatatcgt ctgctttcat ggtttcgtag gcgacgccgc tgggcttgat caccaccagc
    54001 ccgcgttcgc ggtcgatagc gctgacgttg ccccaggtaa aggtcaccag cccatagcgc
    54061 ggcagatcca tgttggcttc aaacacctgc tgttttagct tttgcattat gccgcctcca
    54121 ccatgcccgc tttcgccatg cgcgctttca cccaatcacg cgctttcgcc acttctgccg
    54181 ccgggtcttc cgccgtttcg ctccacatct caatcaggta cggcccgcaa tagccactct
    54241 gtttgagcgt ttcgaagcaa cgttcgaaat ccaccacacc ttcgccaaat ggcacgtttt
    54301 tgaagacgcc cggtttggtg tctttcacat gtaccgcgac gatatgcccg attccggcct
    54361 gcaactccat ctgcacatcg ttgtcccacg ccgacaggtt gccgatatcc gggtagagct
    54421 ggaaccacgg attgttgaga tagtgcgcgt agcccagcgc cttgctgatg gagttcatca
    54481 acggataatc catgatctcc atcgccagcg tcacctgcgc gcggctcgcc atctcaacgc
    54541 tctctttcag gccgtcacgg aaacgacgac gcgtttcgtt attggcttcc tgatagtaaa
    54601 cgtcatagcc cgccagttgg atcacgcgaa taccaacgtc ctgggcgaac tggatagctt
    54661 tacgcataat ctccagcccc tgcgcccgca ccgcgtcatc ttcactgccc agcgggaaac
    54721 gacgatgagc agaaaggcac atggacggca cgcgcacgcc ggtttcaaca atcgcattca
    54781 ccagcgccag acgctgctcg cggctccagt cgaggcgcga caggcgctcg tccgtttcat
    54841 ctaccgacat ctcgacaaaa tcgaagccca acgtttttgc cagttgcagg cgttccagcc
    54901 agcactcccc ggcggggagc gctttttcat agatgccaag cgggatttgt ttggacaaca
    54961 tatccgctcc ttagccccac agttcggcga tggaacgttt gaactgacgt gcggcttcca
    55021 ccggagaagc ggcatcacgg atactacggc ccgcgataaa gacgtgaatc ggaatgcctt
    55081 tgaacagcgg cagatcttcc agcgccaggc caccagtgac ggtgactttg aagcccatat
    55141 cggaaagacg tttgatcgcg gtgatgtccg cttcgcccca cgccacgcct gcggcctgcg
    55201 cgtcacggct gcggtgataa accacctgct gaatacctgc gtcgcgccac tgttgcgcct
    55261 gttcccaggt ccagtaaccg gtcagttcga tctgcacgtc gccgttaaac tcttttgcca
    55321 cgtccagcgc gcctttggcg gtgttgatat ccgcacagca aattaccgtc acccagtcag
    55381 cgttggcttc gaagcacata cgcgaaagga ttttgcctgc atcggcaatt ttggcgtctg
    55441 ccagtacgat tttgtgcggg tagagcgctt tcaggtcacg aaccgcacgc acgccttcgc
    55501 ccacgcacag aatggtgccc acttcgataa tgtcgacttc ttcggcaatc aggcgagtgg
    55561 tttcgtaggc gctatccata gtctggttgt ccagcgcgac ttgcaacatc ggtaatgaca
    55621 tgttcaattc cttcttaagc tgccgcgttg gtgcggtcaa tgagatccag tacttcctgc
    55681 tcggtacggc aggcgcgtaa acggtcaaaa ttctcttcat cttcaaacag gttgacgatc
    55741 tgcatgatgc ccacttcctg gtgagtattg gcatcgaccg ccgccatggt gatgaggata
    55801 tccaccggat cgttgtcatc gtggttgaac tccagcggct ttttcagcgt taccagagag
    55861 aaaccggttt tcttaacgcc ctcttccgga cgcccgtgcg gcattgccag gcccggagcg
    55921 ataacgaaat aaggaccgaa ctgttcaacg ccatccagaa tcgcctggta gtaacgcggc
    55981 tctaccacat ctgccgccac cagcaggtca acgccgattt tcaccgcttc ctgccatgtc
    56041 tctgcttcag cctgcagacg gatggattta ttttcagcca gcgaatcacg taatttcatg
    56101 gcgcgtcctt acttcacgtc ctgcgggaaa tgctctttga tcacttccag cagtttcggg
    56161 ccaaagtcgg caggagagag catgttgcgc acgccaacca cgtatttgtt gccggtcacg
    56221 gtgatttcgc ccgcaatgtg cgtagaagcg atgatgatat ccgcgccact caactcgctt
    56281 ttgtactcgc caaccgcgca gctgtttacc gtatggtcaa tgtttgattg ggttaaaaac
    56341 tggtccactt tcatcttcat gatcatggaa ctgccttgtc cgttgccaca cacagccaga
    56401 atacgtacgg tcataatcaa aactccttat taagcagact gttctgccag ttgtttttct
    56461 gcatcttctt ctgcgcgcag cgcgcggcca gcgaagaaca tataagccag tgcaattaca
    56521 atgatgacgg ccataaaggc gataccgatg gagaagaagc cttgcatcat cggcggtgcc
    56581 agaatcgacc agtccgccat gcccatccag gcactcatac cggtgagttt caccgcccag
    56641 acgcaaccaa agatttcgat cattcccatc accagacaaa tcttcagcgc tgcacgccag
    56701 ccgccgaagt ggttagcgaa cacgccgatg gtggcgttgg agaagaacat cgggataaag
    56761 cctagaataa tcagaatgga agagccgcag gcgaccagga tgccaactgc aatcagctga
    56821 ccgatggtgc cccacataaa gccccagacc acggcgttcg gcgcgaagct atagatagcc
    56881 gcacagtcaa tcgccagaac cgcacctggg atcaggcgct gggagatacc gttaaatgct
    56941 tcagagagtt ccgccacaaa catgcgcacg ccctgagtga tgatgaagat cgccaccgcg
    57001 aaggagaaac cggtttgcag gatgtagacc gtccagtgca ctttgcccgc catcgcctgc
    57061 acggtgtcga taccgaagga gagcagaatg gcgccaaaga agatggtcat cacaatcgcc
    57121 gtggagacga tgttgtcgtg gaaaatgttc agccagcccg gcagtttgag gtcttcaacg
    57181 ctctcctctt ttttgccgag gaacggcgcg actttatagg caatccatga tgcaaactgc
    57241 tgctggtgac cgatggagaa accacagcca tccgtcactt cctgagtcgg cttgtacatc
    57301 atgttggaag tgatgcccca gtagagcgaa accagaatcg cggtacagat aatggtggtc
    57361 cacatggagt agccgaagat aaacagcgta acggcaatca acccggcctg ctggaacatg
    57421 atgtggccgg tcaacatgat tgtgcgaatg ccggtaatgc gacgcaacag cacgtaacag
    57481 atgttcagtg ccagcgctaa caatacggcg taacccaccc agctataggc atcgcccatg
    57541 cggtcgatgg ttgccatcat tgaagcgtag gtatcggaaa ttgcgccgtt aatgccgtag
    57601 acttcggaca ttttcgccac caccggtttg aaggtgctgg tgaggatgcc ggaccctgcc
    57661 tgcaacaaca tgaaaccaat tatggtttta atcgtgcctt taataataac gctgacactt
    57721 ttgcgcagta ggatgtagcc cagacaggtc acaataccca gcaacaacgg ggcattggtc
    57781 atgacctggt taaaaaatac ggtaaagatg ttgtagagga tctccataac gatctcctga
    57841 agaagaggta accgggtatt ccacacaccc ggaatgttgt gcactcactc taattttcaa
    57901 aagtaatcac aacaagatta tttatgatta aatgtgacgc gacccgcaaa taattccaca
    57961 tggatagagc gggattcacg caacgaaaca attattcatt taaattcatt tagttaattg
    58021 aattatacat tccattaaat ctttccatac tcaaaatcaa caacaacttc cctggcaaaa
    58081 agtcaaaaaa tggtcattgc cttccagaaa aaatagtgtc aggattaatc aaaaccaatc
    58141 acatattgat tcgaattgaa ctatcatgaa ggtaaatggc gatgagtaaa gtgaaaagta
    58201 tcacccgtga atcctggatc ctgagcactt tcccggagtg gggtagctgg ttgaatgaag
    58261 aaattgaaca agaacaggtc gctcctggca catttgcgat gtggtggctt ggctgcaccg
    58321 ggatctggtt gaaatcggaa ggtggcacca acgtttgcgt tgatttctgg tgcggcactg
    58381 gcaaacaaag tcacggtaac ccgttaatga aacagggtca ccagatgcag cgcatggctg
    58441 gcgtgaaaaa actgcagcca aacctgcgta ccaccccgtt tgttcttgat ccgtttgcga
    58501 ttcgccagat cgacgcggta ctggcgactc acgatcacaa cgatcatatc gacgttaacg
    58561 tcgctgctgc cgtgatgcag aactgtgctg atgacgtacc gtttatcgga ccgaaaacct
    58621 gtgtggattt gtggattggc tggggcgtac cgaaagagcg ttgcatcgtg gtcaaaccgg
    58681 gcgatgtagt aaaagtgaaa gacattgaaa ttcatgcgct tgatgctttc gaccgtactg
    58741 cactgatcac cctgcctgcc gatcaaaaag cggctggcgt actgccagat ggcatggacg
    58801 atcgcgcggt gaactacctg ttcaaaacgc ctggcggctc cctgtatcac agcggcgact
    58861 cccactactc taactattat gcgaagcacg gtaacgaaca tcagatcgac gtggcgttag
    58921 gatcgtacgg cgaaaacccg cgcggtatca ccgacaagat gaccagcgcc gatatgctgc
    58981 gtatgggtga agcgctgaat gcgaaagtgg ttatcccgtt ccaccacgat atctggtcaa
    59041 acttccaggc cgatccgcaa gagatccgcg tgctgtggga gatgaaaaaa gatcgtctga
    59101 agtatggctt caagccgttt atctggcagg tgggcggcaa atttacctag ccgctggata
    59161 aagacaactt cgagtaccac tatccgcgcg gtttcgatga ttgcttcact attgaaccgg
    59221 atctgccgtt caagtcattc ctgtaatctg tcgtttatac cggatagcgt ggagaagttc
    59281 gcgctatccg gcgtagtatt tcctggctat ttcaaatatc atctaaaaaa atcaaatttt
    59341 atcggaatag ctcatgactg aagcacaaag acatcaaatc ctcctggaaa tgctcgcaca
    59401 attgggcttt gtgaccgttg agaaagtcgt tgagcgtctg ggaatttcgc ctgccactgc
    59461 gcgacgcgat atcaataaac ttgacgaaag cggcaaactg aaaaaagtgc gcaatggtgc
    59521 agaagctatt acccaacagc gcccgcgctg gacgccgatg aatctgcatc aggcgcagaa
    59581 tcacgatgaa aaagtacgta tcgctaaagc ggcctcgcag ctggttaatc cgggcgaaag
    59641 cgtagtcatc aactgcggct ccaccgcgtt tctgcttggg cgggaaatgt gtggcaagcc
    59701 agtgcaaatc atcactaatt atctaccgct ggcaaattac ctgatcgatc aagaacatga
    59761 cagcgtgatc attatgggcg gacagtacaa caaaagtcag tccatcactt taagcccgca
    59821 gggcagcgaa aacagtctct atgccgggca ctggatgttt accagcggaa aagggctgac
    59881 cgcagaaggg ttgtataaaa ccgatatgct gacagcaatg gcagagcaga agatgctgag
    59941 cgtggtaggg aaactggtgg tactggttga tagcagtaag attggcgaac gcgcgggaat
    60001 gctttttagc cgtgccgatc aaatcgatat gcttatcacc ggcaaaaatg ctaacccgga
    60061 aatcctgcaa caactggaag cgcaaggggt cagcattctg cgtgtttaaa gatgctggcg
    60121 gaaaaatgtc acggcagcat ctaacgcctc aggcgtaatg cggtgacgca cgcctggctg
    60181 ccatgaacag gttagcagtt tatcccgtcc cgtctcgctt aaggcctgct gcaaacgtag
    60241 tgattcgtcg gcaggcacaa cgtcatcgtc gaggccatac cacagcagta gaggtctgtc
    60301 actaagttgt tccaggtggt ttgtcgcttc ccactctgcc agtggcgcga caatgttatt
    60361 gaattcattc tgctgtgcta ccgtttcagg tatcagcggt ggaaacagta aacgggcgag
    60421 tgatgtaaaa tagcccgatc ccatcatgct ggcggtacat ctcaccgtgg ggtggcgagc
    60481 ggtaatcccc agtgccgtca tcgcgcccat cgacgcacca ccgactgcca gacggtcatc
    60541 aagcagccac ttttcttcgg ctattgccgc acgtaaagta gtgaattcct gcatactttg
    60601 tagcaagatt tgccagaatt gatttaaccg ccgcgctgcg tcaccactaa aacggctacc
    60661 gtgatcgggc gcatccggca tgatcacccg caaaccagcc tgcgccagcg caacggcaaa
    60721 atagctatac accagactgg acgaagtaaa gccgtgataa aaaatcacgc acggtaacgg
    60781 ggtatctttt tgtccgacag gataagcatg aagaacggga atatctgcca gctcgcgtga
    60841 ttctatttca atcatctgtc tctccttttt gtctggtatg acatgaagaa atccttagca
    60901 caagtttctg ctggcggtat tagttcataa tgttgagatg tgggttacgc tttcgtcacg
    60961 ttttcattcg aaaatcgcgt accaggtaac aattcgggaa cattccccca aagttaaatt
    61021 aataaggcac tacactatgg ttagcaggaa acgtaatagc gttatttacc ggtttgccag
    61081 tttattattg gtgttgatgt taagtgcctg tagcgcactg caaggtacgc cacagccagc
    61141 accgccagta acggaccatc cgcaagagat tcgccgcgac cagacgcaag ggttacagcg
    61201 aataggtagc gtaagcacaa tggttcgggg ttctccggat gacgcattag cagaaatcag
    61261 agcaaaagct gtcgctgcaa aagctgatta ttacgttgtc gtcatggtgg acgaaaccat
    61321 cgtgacagga cagtggtatt cacaagccat tttgtaccgt aaataaatca gacaacttac
    61381 atagtcagct ttacattgct ttgcgtccct atgcgtttac ctgtgcacaa taaattaccg
    61441 gctggaagcc tgggcgttta ttaccgcgat ggaatgccct gcaacatgtg gggaaacgaa
    61501 aaatggagct gacgatgaaa caattacttg cctcaccctc gctgcaatta gtgacttatc
    61561 ctgcgagcgc cacggcacag tctgcagaat tcgccagtgc tgactgcgtg acgggcctga
    61621 atgaaatagg tcagatctca gttagcaata tttcagggga tccgcaggac gtggaacgta
    61681 tcgtggcgtt aaaggccgat gaacaaggtg cgtcatggta ccgcattatt accatgtacg
    61741 aagaccagca gcctgataac tggcgcgtac aggcgatact gtatgcatga gtgtcatcta
    61801 cagcaaatga acaatgccgg atgcggcgcg agcgccttgt ccggcctaca attttcacat
    61861 atttcattta gttaatcgaa accagcgtcg catcagtcgt atacgcttac acacacactc
    61921 cccccgtcgc ccgcagcaat aaatcattct ggatctgctc tgacaaccgt acgccgccgc
    61981 gcgtatctaa catcacctga caccacgcct gcgccattgg cggagaagca tatttcaaca
    62041 tttgcgcacc gcagcccagc aggaatagct gatgagtaat ctctcgcccc agttcttcag
    62101 ctggcttacg cagctgctgc tgtaaacgac gaaccgcgca atcaaaatag cgatcctgcc
    62161 ctttcacttc tacaaatgct tccgacaata agtcgtatac gcccgcttgc ttattgagaa
    62221 cgcgcagcac atccaggcac ataatattgc cggaaccttc ccaaatactg tttaccggca
    62281 tctcccggta aagccgcggc agctcgctct cctcgcaata accaatgccg cccagcacct
    62341 ccatcgcttc ggccacaaac ggcatacctc gtttgcagat cacaaatttt gccgcaggcg
    62401 taaataaacg cgcccacaga gcttctttgg catcggcgcg ccggtcccac gctcgcgcaa
    62461 gacgaaacag caacgccgtt tgcccttcaa gctgaagtgc catgcgactt aagacatgac
    62521 gcataagggg ctgttggatc aatggattac caaaaacatg gcgttgatgc gcatgataaa
    62581 tcgccagcga gaacgcacgg cgcatcatgg catggctacc cagggcgcaa tcaaaacgcg
    62641 tcatcccccc cattttcagg atcagacgaa ttccttcccc ttcctgcccc aataaccagc
    62701 caatggcatc ctgaaactcc acttcacaac tggcgttaga gcgattaccc agcttatctt
    62761 tcagccgctc gaggcgaatc gcgttgcgtt gcccgtcagg caaaaagcgc ggcacaaaaa
    62821 agcaggacaa accgcccgcg gtctgcgcca gcaccagatg cgcatcgctt tgcggaactg
    62881 agaaaaacca tttatgcccc accagccgat aagagccatc ctccagacgc tctgcgcggg
    62941 tggtgttgct catgacatcg gaaccgccct gcttttccgt cattcccatg ccaatcaaca
    63001 aaccgcgttt ttgcccacct ggcaataagt gagaatcgta gcgatcgctc aacagcggtg
    63061 tggtccagtc ctgaaacggc gcgggtaaca tctgtaacaa caatggcgtg gcggcaaagg
    63121 tcatggttat cggacataac gaccccgcct caacctgtgc gtgcaacata aaacgcgccg
    63181 cgcgcgccac aaatgcgccg gagcgagcgt cttcttccca ggcaagattg tgcacccgat
    63241 tggtacatag tgcctgcatc agcaggtgcc aggcggggtg aaaacgcaca tcgtccagac
    63301 ggcgtccttg cgcatcgtag cgcaatagtt caggcggatt cacattcgcc agccgcccca
    63361 gttcaaggga ttcagccgtt cctaactgct gaccaatact ggctagaaaa tcgctatccc
    63421 agccagcacc ttcacgcgtt accgcttcgc aaagcgcgcc atcagacagg tataagttgc
    63481 tgttatttaa tggtataggt tgattaaaaa cggtgtgagt ttgccagtgc actgtgtctc
    63541 cctccatcaa tggcagtcac cattagtatg gtcactgcca tggattcatg acagaaagag
    63601 attgctaaaa cattcttatc cagcaatgta atgcggaacg aaacgtgagg tgtctttggt
    63661 gatcagtgtg ttatcttcac ggatccccat tccgcacgct tcatcatcga caatccagct
    63721 accgatgagt gtgtagctat cgccaaaccg cggcagaggt tgaaacgcct ggtagatcat
    63781 cggttcatcg gcgtaatcac catcagcgtg gtcaacgacg ttattctggc cgtcgaaaat
    63841 ggtgacattt ccgccttcgc gcgagtagat cggtttacgc acatagcttt cgccagcggc
    63901 aatctgcggt ttttcaccct caaaccagga tgcaagaagg ttaggatgac cagggaagaa
    63961 gcgccaaagc agcggcatta gccctttatt actcaagata cttttccata acggctccac
    64021 ccattgctcg cgacgcttgc gcagcagcgg accgttatcg tcacgcatca tccactccag
    64081 cggatacagc ttaaatgcac gctggatgac attatcatca agatcggtca gtacgccgcc
    64141 gacgcccaaa ccgagatctt caatgtagat aaaccgcgac tcctgccctg cctgctgggc
    64201 gcagtcctgc aagtacagca cggtactcct gtcttcatcg gtgtcctgac agcagcaaaa
    64261 ataaaacggt tcccgactgt aaagctcgct aaagcgtgaa atcaggcgtt cctgaatagc
    64321 attgtactga tcggcatcac gcggaataat gccgctgcgc cgggcatcct ccagccacag
    64381 ccactggaaa taagccgact cgtacaatga agttggcgta tcggcgttgt actccagcag
    64441 cttcaccggc gcattgccac accaggcaaa atccatccgt ccatacagcg aaggatcgcg
    64501 ggcgcgccag ctttcagcga tcacatccca gtacaacggc gggatcgcca gttgcgtcag
    64561 gatctcttca tcttttaccg cgcgatccac cacctcaagg cacatctgat gcagttccgc
    64621 agtcggtttt tcgatctgct cttcaatctg gcgcagagta aaacggtaag cccgactctc
    64681 atcccaatag atttcattgt cgatgatatg aaagtcgaaa ccgttgtcag cggcgatctg
    64741 gtccagatcc cgtcgcacag gaacgttgtg tctcagcatg attagcctcc ccaatgccca
    64801 cgggcgctgg aagaacgacc atagccgccg cgagaaacgg ttgacgcttt tttggtggta
    64861 aagccgcctg aagagtaaga ctcttttttg ccagaaccgg agcgccagga gtaatcaccg
    64921 gaagtgttgc gccagacggg gcgggaagca aatgaggatc cgccgctgtt atagacaaac
    64981 ggctcatcgc gatctttacg aataacccgg cttaacaaaa atccagaaac gaccgggatc
    65041 caactctgtt caacattgtc gtagtagcaa ttttcgtact tagactgaca gttctgctga
    65101 gtcatattct tcggaacatc ggcataaaat gccgctttgg cgttgttcca gccacgcgcg
    65161 caaatgtcgg cattattacc gtcatcaata caatcctgca ctgtcgaata aaacgttccg
    65221 tcgccgtcgt tatcgacatc gccactatcg ccacaacctt tcaatacaaa aaaagcggca
    65281 ccgcccatta gcgccaacgt taagtatttt tgtgcgtagc gattgcgacg cggttcaaaa
    65341 ggattattcg gtctaccaat gcgactgatc gctccgtgac caattttact gttatttctt
    65401 gatttgcgtt ttctggccat gatcgtccat taccatgtca tacaagctgc gttaaaaata
    65461 ccgccagcca gcgcggcggt tcccataaac attcctgctg cggtattgtg attaataatt
    65521 ttttcgctta atgcgggcat ataaagtctg acaccagcaa aaacgagtaa ctgaatcacc
    65581 agtgcaatcc cgccccaggc gaaatagtct ggaatactca ccgcattgat tgccgcactg
    65641 gataagggga tcacgtaacc caacaatgta ccgctgaacg ccagtgacgc tgcggtatta
    65701 ttgtttttga ttaattgcca ttcattgtgc ggtgtaattt tggagtagat aaacagaaaa
    65761 ataatcacca tagccacgcc aataaaaaaa taggcactaa aggcaagaag tgaatccagt
    65821 atatgcattg taaacgtcct tgtaattaac caataatatg aagtgatgtc agcgggatat
    65881 cgacacccaa cgcgcgcgaa aataaccact ctggctcgcc gagatcgtta aaagattcct
    65941 caccatttag cagcaaatat tcgtaagtat cttcggtcac ttgccgctgg tagcccatgg
    66001 taaaattatg gacttcccat ttagcatgat tttgattttc tactttttcc agcatgtaga
    66061 cgggttcgat atttcccggt tcttcgctgt taaaaaatcg ctgccagcgt ttttcctgcc
    66121 agtttaaggt cattgccccc atcgttttgg cgttgatcgc ctcgcgccag tgattctctt
    66181 tactgatacc gtagctctct tcatagacaa agagcttgat atcatcaatg tcatcaatat
    66241 cttcgccgcc ggtggtattg atttgcagaa actcatcgcc tgaagtgtag tagcggaaaa
    66301 tctgactacc gccgcccaga tcgatgtggc tgacagcgac taccgtaaat tcctcacccg
    66361 gcagcgcaat cagcaattca tcttccagca aacgaaacgc taacgtgtcg agcgtaaagc
    66421 cactattgag atgaagtccc agcggaccgc gagcgatagc aggcttatta tccttgccaa
    66481 acagacgctg gaaaaaacca gacatatttt atccttaaaa tagttgccag ccttgcagtg
    66541 aggctggcaa aaaaattact cgccctgttg gcgttgcagt cttgctaata catcctgggc
    66601 gctactttta ttgctaccgc caattccggc ttccgccagc ttttcgtcaa ggtcgcgacc
    66661 gtctgcgact ttttctaact gtgcggcagc atccaggcga gcctgacgtt cggcctgacg
    66721 cgtttgcagg cgttttaagg attctgctgc tgtcgaaacg ctggaagatg cgccaacggt
    66781 agaagtcgtt acagcctgtt gtgcacgctg catggcttca gtggctttaa cgacttccat
    66841 ttgctgctca aactgagcga tacgctgcgc tgtcgctgca actgcttttt ccacgccatc
    66901 acgggagact tccagattcg acaacacttg ctcttcagcg gtaatgagat tctcaaggcg
    66961 cgcgatttct tcagcaactt cgttaatcaa cgacggatta acgttcttgc ttaacgcttc
    67021 cagcgtacgg gcttccagac tggctttgcg ctcacgtaaa tctttcagct tatcgtgact
    67081 taatttcacc cgcgccagca gatcaacgcg agattttccg gctttatcga gttcagcttt
    67141 agcgtcacga atatgctgtt ccagcatgcg cacaccctgg gtttcttcaa tggattcttc
    67201 cgcctgggag ataaacgatt tccccagcgt aaataaactt tttaaaattc ccataaatac
    67261 ccgtccttgt aattagtgtg aatattcttc tgtaatttca gccagatcca atgcgttatc
    67321 taccagcgag gttatctcaa gcaggatatc ttcaagagaa gatttaagcg ataacgcccc
    67381 gaaaacaatg taatactctt cttgttgtac gctggagatt cctaccgatg acaacggcat
    67441 catcttctga tttcttaata agaaggtatt aaattcatct ggattgctga tactactcac
    67501 cggacaaata aaggtttcga taatcatttg gcgggaagta aaaagaatat tgatttgcaa
    67561 atcgccataa tcattcattt taataattaa tgcgttctcg ctatttgtta catcaatatt
    67621 ttgttcaggt acagtttgca gcgccgtcgc cagcgccaac ggattccatg tcatatatta
    67681 ttccatatag attaagttta aatattaata aaatgaatat ttgcaatacg taattatctt
    67741 accagctata gacaaaaaaa aaccatccaa atctggatgg cttttcataa ttcagaggaa
    67801 ctagctgcgc tgacgaaccg cttcaaataa gcaaattccg gttgcaaccg aaacgttcag
    67861 ggaagaaaca cttcctgcca ttgggatgct gatcaactca tcgcaatgtt cacgggtcag
    67921 gcgacgcata ccttcacctt ccgcgcccat caccagcgcc aggcgtccgg tcattttgct
    67981 ttgatagagc gtatgatccg cctcacctgc cgtaccgacg atccagatat tctcttcctg
    68041 caacatacgc atggtgcgcg caaggttagt cacccgaatc agtggaacgc tttctgccgc
    68101 gccgcaggct acttttttcg ccgtggcgtt gagctgtgcg gagcgatctt tcggcacaat
    68161 caccgcgtga acgccagcag cgtccgcgct acgcaggcac gcgccgaggt tgtgcggatc
    68221 ggttacaccg tcgaggatca gcaggaacgg ttgatcgagc gaagcgatca gatccggcag
    68281 atcgttttcc tggtactgac gtcctggctt cacgcgggcg ataatgccct gatgcacggc
    68341 accgtcgctt ttctcgtcga gatattggcg gtttgccaac tggataacca cgccctggga
    68401 ctcaagggcg tggatcagcg gtaacagacg tttatcttca cggcctttta aaataaagac
    68461 ttcctgaaaa cgttcagggg cgcgctccag cagggcctgc actgcatgga tgccgtaaat
    68521 catttcgctc attaatgtac tcgttgttta cgttttgcct gatgcactac gtttatccgg
    68581 tctacatgat ccctgcaata tattgaattt gcgtgattat gtaggccgga taaggcgttc
    68641 acgccgcatc cggcatgaac aaagcgcatt ttgtcagcaa tctaaccctc ttctgtttga
    68701 agagggtaat gatcactctg ccactttttt cttcgccgca cgcttcgctt tggtcgctgc
    68761 agctattttc tgcgttttcg ccgatggctt tttcgctttt ctcgcgtctt tcttcgctgc
    68821 tttcggcttc gtttttttct caccgcggaa ggcgctgtct ggctcaaagt ttaccttttt
    68881 accgacctga cgacgcttgc cgcctttttt acctgcatcg ccttttttcg ctttctcgcg
    68941 cgccgtttta ccgacgttgc gcggtgcgcg ttcgctggag atcaggctaa agtcgatttt
    69001 gcgctcgtcc atattaaccg cttcgacgcg aacttccacg cgatcgccca ggcgataagt
    69061 ctggccgctg gattcaccca tcaggcgttg ccctacctgg tcaaagcgat agtagtcatt
    69121 gtccagcgaa gagacatgga ccagcccatc aatgaacaag tcgtccagac ggacgaagaa
    69181 gccaaagcca gtgacgctgg aaattacgcc tttaaagacg ttacctacct ggtcgagcat
    69241 gaagtcacac ttcagccagt cagacacatc gcgcgttgct tcgtcggcac gacgttccgc
    69301 catcgaacag tgctgaccca attgcagcat ctcttccatc gaataatggt agccaccggt
    69361 ttcagtggtg ttgccctgat gcccctgctc tttcgccagc agatacttaa tggcgcggtg
    69421 cagcatcagg tcaggataac gacgaatcgg cgaagtaaag tgcgcatagg actgcaatgc
    69481 caggccaaag tgaccacggt tttccggatc gtaaatcgcc tgtttcatcg agcgcagcag
    69541 catggtttgc agcatttctg catcaggacg gtcggcaacc gactccagca gctccgcgta
    69601 gtcgcgcggt tccggcttgt taccgcccgg cagttccagc cccagctctg ccagcactga
    69661 acggaaagag gtaatcgctt cggtgctcgg cttgtcgtga atacggaaca gtgccggttc
    69721 tttcgctttc tcaacgaaac gcgccgccga gatattcgcc agaatcatgc actcttcaat
    69781 taacttgtgt gcgtcgttac gctgagtctg ttcgatacgt tcaatacggc gttcagcgtt
    69841 gaaaatgaac ttcgcttctt cgctctcaaa tgagatcccg ccgcgttctt cacgggcttt
    69901 atccagcact ttatagaggt tatgcaactc ttcgagatgc ttaaccagcg gggcgtactg
    69961 ctcgcgcaga tcctgatcgc cctgcagaat atgccagact ttggtgtagg tcagacgcgc
    70021 gtgagagctc atcaccgctt cgtagaattt gtagccagtc aggcggcctt tcgacgaaac
    70081 cgtcatctcg cacaccatac acaggcggtc tacctgcgga ttgagcgagc acagaccgtt
    70141 agagagcact tccggcagca tcgggataac ctgcgaaggg aagtacaccg acgtaccacg
    70201 gttacgcgct tctctgtcca gcggcgttgg cggacgcaca tagtagctga cgtcagcaat
    70261 cgcgacccat aaacgccagc cgccgccgcg ttttttctcg cagtaaaccg catcgtcaaa
    70321 gtcacgggcg tcttcgccat caatggtgac cagcggtaaa tcgcgcaaat cgacacggcc
    70381 cgcttttgct tcttccggca cttcttcttt cagcccagca acctgttgct caacagcctg
    70441 cggccagatg tacggaattt catgggtacg cagagcgata tcaaccgcca tgcccgtgcc
    70501 catattgtcg cccagcactt cgacgatttt acccaccgct ttggtacggc gagtcgggcg
    70561 ttgggtcagt tcaacaacca ccacaaagcc catccgcgcg cccatgatct gatcgggcgg
    70621 gattaagata tcgaagctca gacggctatc gtcaggaacc acaaagccga cgcccgcttc
    70681 ggtaaagtag cgaccaacaa tctggctggt ttttggcacc agtacgcgga caatacgcgc
    70741 ttcacgacga cctttacggt cagcacccag cggctgagcc agcacctgat cgccatgaat
    70801 gcaggttttc atctgctcgc tggagagata caaatcatct ttacgccctt caacccgcag
    70861 aaagccgtag ccatcacggt ggccaataac ggtacctttc accaggtcga ggcgttccgg
    70921 cagcgcatag cactgacgac gagtaaagac cagttgacca tcgcgctcca tcgcgcgcag
    70981 gcggcgacgc aggccttcaa gctgctcttc gccttcaatg tgcagttcta ccgccagctc
    71041 atcacggctg gccggttttt cacgtttggt taaatgttcg aggataaatt cccgactagg
    71101 gatgggattc gcgtattttt cagcttcgcg ttcctggaaa ggatcttgtg acatctcggt
    71161 tcctccgttg tcatctctga tgaagatttt cgtcactcca ccagcaataa tttataaagc
    71221 ggttgattct cttcaaccaa atcggcaagc gtgtagttat ccagttccgt aagaaaactt
    71281 tgcacggcct tagaaagcgc ctgtttcaac cgacaggcag gtgtaatgtg gcaaaactca
    71341 ctgctgcaat tcaccagcga taagggctcc agctcgcgca ccacatcacc aatacgtatc
    71401 gcactcgccg gtttacccag gcgaatgccg ccattttttc cacgaacagc agtcacgtag
    71461 ccggcacgac taagttgatt gattattttg accatatgat tacgggagac gccgtagacg
    71521 tcagtcactt cagaaatact ggtcatccgc ccttctggca atgacgccat gtagatcagc
    71581 gcacgtaatc cgtaatcagt gaaactcgtt aactgcacat caacctcaaa agggaaatcg
    71641 ggaaaaaata catttatatt gatgataaac cagccacaag ctgtgtcgct aatttatttc
    71701 agttcgggga aggaaaaaag cgaggattta acactgtgtc ggatagcgta atggcttaac
    71761 cgacacagca atgtatcaca ctgttagccc ggcaggcaaa atatctgcca ggcgtaccag
    71821 aattacgcgt cgaacgggtc gcgcagaatc atggtttcag tacgatccgg accggtagag
    71881 atgatatcga tcggtacacc ggtcagctct tcaatgcgct tgatgtagtt cagcgccgcc
    71941 tgcggcaggc cgctacgatc tttcacgccg aaggtggatt cagaccagcc cggcatggtt
    72001 tcgtaaatcg gctctacacc tttccagtcg tcagctgcca gcggagtggt agtcacttca
    72061 cgaccatccg gcatacggta agccacacag agtttcacct ctttcaggcc atccagaacg
    72121 tccagtttag tcaggcagaa gccagacagg gagttcagct gtaccgcacg acgaacggca
    72181 acggtgtcca gccagccggt acgacgacga cgacccgtag ttgcgccgaa ttcgttaccc
    72241 tgcttgcaga ggaactcgcc agtttcatca aacagttcag tcgggaacgg acctgcaccc
    72301 acacgagtgg agtaagcttt gaggataccc agaacgtaat caacataacg cgggcccagg
    72361 ccggaaccgg tcgccacgcc accagcagtg gtgttggaag aagttacgta cggataagta
    72421 ccgtggtcga tatccagcag cgtaccctgc gcaccttcga acatgacgaa atcgccacgc
    72481 tgacgcgcct ggtcgagcag gtcagaaacg tcaaccacca tagaagtcag gatgtcggca
    72541 acagccatcg tatcatccag aactttctgg taatcaaccg cttcagcttt gtagtagtta
    72601 accaactgga agttgtgata ttccatcact tctttcagtt tttcagcgaa ggtttctttg
    72661 tcgaaaaggt cgccaacacg cagaccgcga cgtgccactt tatcttcata agcaggcccg
    72721 ataccacgac cggtggtgcc gatcgctttc gcgccacgcg ctttctcacg cgcgttatcc
    72781 agcgcaacat gataatcaag gatcagcgga catgcttcag acagcagcag acgctcacga
    72841 acggggatgc cacggtcttc cagttctttc atctctttca tcagcgcggc cggagacagc
    72901 acaacaccgt taccgatgat gctagttaca ttctcgcgga gaatacctga tggaataaga
    72961 tggagaacgg ttttttcacc gttgattacg agagtatggc ctgcgttgtg accgccctgg
    73021 tagcgtacaa catatttagc ccgttcagtc agaagatcga cgatcttacc tttaccttcg
    73081 tcaccccatt gggtgcccag tacgacgacg ttgttaccca tttttcaaaa tcaccgtttg
    73141 cttaaaaatg gattctacca tcgctttttc agagttacag cactttttgc actcaaaaat
    73201 gacctcgatc agtctgtttt ttgttcagcc aatcgttttc ctcaacatgt agtagaccac
    73261 aacgcccgca accacaagtc caccgccaaa acgacgtaaa atattatcgg gcaaattggt
    73321 catcgcagag atcatcttct tccatgcctt cgggtaaagc atcggcccta aaccttccag
    73381 taccaaaacc agggcaagcg ccagccagat tgtcgaattc attcattatc cttatagaaa
    73441 aagaaaacca ccgacatccc tgaggatgcg gtggctttat tgacctgtac cgcagtcgtt
    73501 atattaacgc gttgcggaag tcggcgtctt catgtagcgg aagaaatcgc tatccgggct
    73561 catgaccatc acgtcctgat tgccagagaa gctgttctca taagcacgca ggctacggat
    73621 gaatgcgtag aagtccggat ctttactgaa tgcatcagca aacagtttgg ctgcttcggc
    73681 atcgccttca ccgcgcatga tgcggccctg acgctcagct tctgccagcg ttctggtcac
    73741 ttcatagtcg gcagtcgcgc gcagtttttc cgcttcttcc tgaccttgtg aacggtgacg
    73801 acgcgctacc gcttcacgct cggcgcgcat acggttgtag atcgcttcag acacttcggt
    73861 cggcaggttg atctgcttaa ttcgcacatc gacaacttca atacccagcg ccgccatact
    73921 gttcgggttg atgaccggaa ctttgccctt cgtctcagcc gttacgcgct ctgccgcttc
    73981 ggcaatggcg ttatctgccg ccggggtagt aacttcatct tctgtacccg cagaaccgga
    74041 gttcagcgcg tcacgtactt cgagggtcag acgaccacgg gaatcggtga cgatgtcttt
    74101 cacgtccagg cgaccaattt cagaacgcag acggtcagag aacttacgtt tcaacagcac
    74161 ttccgcttgc gaaatgtcgc ccccccccgt tgccaggtag taacggctga aatcgctgat
    74221 gcgccatttg atgtaagagt cgacgatcag gtctttcttc tctttggtca caaagcggtc
    74281 ggcctggttg tccatggtct gaatacgtgc gtcgagcatt ttcaccgttt caatgaacgg
    74341 tatcttgaaa tgcagacccg gctcataaac cagaggtttg ttgtcatcgt cacgcagtac
    74401 cttaccaaaa cgcagcgtaa taccgcgctc accttctttg acgacaaaga cagacatgta
    74461 aagcactacc agcacgatga tgataatcgc gataactgac ttacgcatcg ttattccccc
    74521 tgacgctggt agtcgttacg ctgcgcattg gcgcggcgtt ggtccataat atcgccctga
    74581 ctggtggacg acgtgttgct tgctccactg gttgtggagg aagaggctgg cggcagacgc
    74641 agcagattgc tggcaccgtt atcgctcttc gccgcagggg cgttaccacc tttcagcatc
    74701 tggtctaacg gcagaaccat caggttgcca cctttatcgt taaccagcac tttgcgggtg
    74761 ttacccaaca ctttttccat cgtctcgata tacagacgct cgcgagtaat ttccggcgcg
    74821 gctttatatt ccggcagaag tttagcaaaa cgcgccactt caccctgagc ttccaggatg
    74881 gtctgggcct tgtacgcacg cgcctcttcg aggatacgtt gcgcctgacc gttcgcacgc
    74941 ggctgaactt cgttggtata cgcttctgct tcacgaatgt attgctgttc gttttcacgc
    75001 gcggcaatcg catcgtcaaa cgccgctttt acttcttccg gcggacgagc agcctggaag
    75061 ttgacgtcca gcagcgtgat acccatgtca tacggacgaa tcgtctcttc cagttcgcgc
    75121 tgagtatcgc tacgaatcac ggtacgacct tccgtcagaa tgcggtccat ggtgtattta
    75181 ccgataactc cacgcagggc gctgtcggta gcctgacgca ggctgtcatc cgggctggtc
    75241 acgctataca gatatttttc cggattggtg acgcggtact gcacgttcat ctcaacgcgc
    75301 actacgttct cgtccgacgt cagcatcaca ccagaagctg ccagttcacg cacggcttcc
    75361 acgttcaccg gtttgacttc gtcgataaac gtcggtttcc agttcagacc tggctcaacc
    75421 agatggctga atttaccaaa gcgtgttacc acgccgcgtt cggcttcttt aatggtatag
    75481 aaaccactgg ccgcccagat aatgacaatc gctgccgctg cgatggtaac gacacgaccg
    75541 ccaagctgcg ggcgcgggcc ttgcgatgaa ctgccaccgc cagatccggt gcctttaccg
    75601 ccgcccagac caccgagctt tttgctcagt ttgcggaaga tatcatctaa atcaggtggc
    75661 ccttgatcgc gaccgccttt gtttccattt ccctcagagt tgccgccagg tttgctgctt
    75721 ccccacgggt cgcggtcttg tccgttatta ccgggctgat tccacgccat gtttgtgctc
    75781 catatttgtt atgcggtgat ccctgttgtc tttcgacgcc agaggatatg actccacgct
    75841 tcagacgcta cgccgttaga tcaggtaatc gatcaacgcc ggttcttgtt tacagaggcg
    75901 acgccagtca acgatcggca tacgaacttg cagacttacg ctgccgtcct cctccatcca
    75961 ctctttttct attgcctgaa gctgataaaa acgacttctc agacgccctt cctgcggtgg
    76021 cagacgcaat gtatgctgcg ccacctcgcc ggaaagccgc tccgtcaaag cctgaaaaag
    76081 ctgtggtatc cccgctccgg tctgtgcgga aagccagaca cggatcggtt tgttctcttc
    76141 gtcccgatca atacgcggtt cgaaatcttc cagcatatcg atcttgttca tcaccagcag
    76201 ggttgggatc tcgtgagcgt cgatctcttc aagaaccgta ttcaccgctt cgatgttttc
    76261 ttgtacacgc acatccgccg catcaatcac atgcagcagc aatgtggctt gccgcgtctc
    76321 ttgtaacgtg gctttaaatg ctgccaccag atcgtgcggc aggtggcgaa taaaccctac
    76381 ggtatctgca agtacggttt caccgacatc tgcaacgtca atacgccgca acgtcgggtc
    76441 gagggtggca aacaactggt ctgccgcgta gacccgcgct tcggtgatgc gattgaaaag
    76501 ggtagattta ccggcgttgg tatatcccac cagcgaaaca gtaggaacgt cggctttgat
    76561 acgcgattgc cgcccctgct cacgctgctt ttcaactctt tccaggcgcg actgtatctg
    76621 cacgatgcga ttacgcaaca aacgacggtc ggtttcgagc tgggtttcac ccggaccacg
    76681 caaaccaatc ccgcctttct gtctttcaag gtgggtccag ccacgcacca ggcgtgtagc
    76741 cagatggcgc agctgcgcca gctcaacctg caacttaccc tcatgggtac gcgcacgttg
    76801 ggcgaaaata tctaaaataa ggccggtgcg gtcgataaca cgacactcgc acaaacgctc
    76861 caggttacgc tcttgcgccg ggctcagggc atggtcaaaa agaacgaccg aagcacccgt
    76921 cgctttgaca gcttccgcaa tttcaactgc tttaccttca cctacaaaat actttgggtg
    76981 cggcgcttta cggctaccgg taatcacctg caatgcttcg acaccggcgg aagagaccag
    77041 agattcaaac tcctggaggt cttccatatc tttgtcttgc gtaaaataga tgtgtaccag
    77101 taccgcctgc tcaccagcat cataacggtc aaacaagcgt ataaccctct aaatagatca
    77161 gcggggaacg caggatcgct ggctccccgt gtaaaaaaaa cagcccgaaa ccttattcgg
    77221 tttcttcgct gtcctgttgc gcggaagtat tctgcgcgct gctaccatga tggtagttac
    77281 tgctggtacc gccaccggcg ttgttactgt gatgagaaac cgggcgagac gggacaacag
    77341 tagaaatcgc gtgcttgtaa accatctggc tgaccgtgtt tttcaacagg atcacgaact
    77401 gatcaaaaga ctcgatttgc ccttgcagct taataccatt caccaaataa atagaaactg
    77461 gaacacgttc ccgacgcagt gcgttcagga acggatcttg taaagattgc cccttagcca
    77521 ttctctcttt tccttatatg cttatttgta ctttgaacct ttcgattctg aaaaaattgc
    77581 gcacgatacg tctcaattgt acacattcag cctgcgatag caccaacaac ctgtaatact
    77641 tcgtcacgcg cctgttctgg tttttcactg tcaagccagt gaaccccttc ccaaccacgc
    77701 agccaggtta tctgccgctt cgccaactgt ctcgtggcgc aaacacctcg ataaaccatt
    77761 tcatcgtatg agatttcgcc tccaaggtaa gaccacatct ggcgataacc cacgcaacga
    77821 atggaaggca agtccgtatg caaatctcct cgggcaaaaa gcgcccggac ttctgcttca
    77881 aaacctgaag ccaacatctg atgaaaacgc tgctcaattc gttgatggag cagttcacgg
    77941 ctcgccgggg cgatggcgaa ctgatgcacc tgatacggta gagcgtctcc tgacgtttgc
    78001 gtcagttccg ttaaagtttt acccgaaatg aaaaaaactt ccagtgcccg ggaaagcctt
    78061 tgtggatcat ttggatgaat ccttgctgcc gcaaccggat cgatctcctg aagttgacga
    78121 tgcaatgact cccaaccttg ctctgccgcc tgttgctcaa ttctggcccg tacttccggg
    78181 tctgccgacg gtagcggcga caacccttcc agcaatgcct tgaaatacaa catcgtaccg
    78241 cccactaaca gtgggatccg ccccgccgcg gtgatatcgg ccatttccgc cagcgcatcg
    78301 cggcgaaaat cagcagccga gtaagcctgc gacggatcgc gaatatccag caatcggtgc
    78361 ggcgcggcga gtaactcttc agcgttcggc ttcgccgtcc cgatatccat ccctttgtaa
    78421 ataagggcag aatcaacgct tatcaactct actggtaaaa ttttacgcag ctcaatggct
    78481 aacgccgttt taccggaggc cgtcggcccc atcaaaaaaa tcgccttagg caggctcgcc
    78541 ttactgatat cactcatctt tcagggcttt tatcgccgga tgtaaatcaa cagattgtaa
    78601 cagaccaccc ggcggcgttt tcacaagttg cggacataac cgttccacgt ccgccagcag
    78661 ggttatggcc tgtgccattg accactgcgc atgttcgctc atcagatttc gtgcaatcca
    78721 ctgcgcaata ttgccaggtt cgaatacgga ctgcttcgct aggtagccta tcagttcagg
    78781 aatcaagatt tgtaaatttt gttggcgtaa gggtaaaggc acggccctga tggtcacatg
    78841 ctgtgcatct gactggaaat caatacccaa ttccgccagg gcagactgcg ctttttctaa
    78901 tgccgatttt tcttcgccag aaacttttag ccgcaacgga atcagcaacg gctgggcgca
    78961 aacgggcgct tcacccggcg tcagttgtac ctgacgcagc caacgttctg ccactggcaa
    79021 ggctaaaagt gaaatgttgc cgtcgcgctc cagcaacgca cagtcggaat ggacgatagt
    79081 cagtacccga ccaaaactct gactgttcgc cgcaagtgca ggttcctgcg gttccggcgc
    79141 ttttggtttt tgcatcggcg cgggcgtttg caaaagctgg cgatacactt caccttgctg
    79201 tttctggtaa cctggctgcg cattcggcca gggggcagcc ggacgactac ctgatgctgg
    79261 cgcaggagtg tagcgcggag ctactggctc acgaactgcc ggttcagcaa agtgattgcg
    79321 ccccgccgcc acgcggtttt ccggaatggg acgcggtgca ggttggggtt catcgtccag
    79381 cggtagcggc gtttccagtt gctgttgtag cacgctcagc acgccctgat agataaagtc
    79441 atgcaccaga cgcgactgat gaaaacgcac ttcgtgtttg gcggggtgca cgttgacgtc
    79501 cacctgatgc gggtcgatct ccagatacaa cacaaatgca ggttgctgat cggcccccag
    79561 tttgtcttca caggcctggc ggatcgcgtg attgatcagg cgatcgcgca tcatgcgacc
    79621 attcacgtag caatactgaa tttccgccag tgcgggcgtg gtgtgatttg gatcggccac
    79681 ccagccgcgc agcgtgagat cgccgtgttg ccattcaatc gccagcgctt gttcgagaaa
    79741 agcggtgcca caaatcgcgc ctaagcgccg ttctttttgc ccgccttccg gcaccgcgcg
    79801 gtactggcgt acgattttgc cgttatgcga caggttgatc gtgacgtcga aacgcgccag
    79861 tgcgatgcgg cggatgattt catcgatatg gttaaattcg gttttctcgg tgcgcaggaa
    79921 tttgcgccgc gccggggtgt tgtagaacag gtccagtacc tccagcgtcg ttcccaccgg
    79981 atgcgccgcc ggtttaaccg tcacgtccat atcgcgccct tcggcatagg cctgccaggc
    80041 ttcctgctgt tctgcggtgc gtgaagtgag cgtcaggcgg gaaaccgaac tgatactcgc
    80101 cagcgcctca ccgcgaaagc ccaggctgat aatggcttcg agatcgtcca gagaggcgat
    80161 tttactggtg gcatgacgcg ccagcgccag cgccagctcg tcttttttga taccacagcc
    80221 gttatcacga atgcggataa gtttcgcccc accgcgttcg atatcaatat cgatacgcgt
    80281 cgcacctgca tcgaggctgt tttccaccag ttctttgact accgacgcag gtcgctcgac
    80341 cacctcacct gcggcaatct ggttcgccag ttgtggcggt aagacctgaa ttggcatcaa
    80401 tcgtccttag tttggcagcg tgcgatctgg cgtcgtcacc gtactggcag tttgtgccgt
    80461 tgcaccctgc ggcgcagatt gcatcggatg cgcaaggaaa taattgcgca ggcctttgta
    80521 aatggcttct gccagctgtt gttggtaatc gtcgctcgcc agcaaacgtt cttcgctgtt
    80581 gttgctgata aaaccggttt cgaccagtac tgatgggata tccggcgagc gcagaacgcc
    80641 aaggctggcg tgttctggtc gacgtttatg tatttcgcca atgcgttgca actgactgat
    80701 catactggtc gctacatcat accctacccg ctgggaatga ccgaactgta aatccagcac
    80761 cgcctggctt aaataggggt cagactgact gttcgccagc acatcacccg ccccgcccag
    80821 cagctccgac tgtttctcgt gctgctccag ccagctggcc atttcgctgt tggcgcgacg
    80881 gttagagagc acccatacgg aagcgccagt cgcactgcgg ttcggtgcgg catcagcgtg
    80941 aatcgacacg aggaaattgg cgttttgctt acgtgccaca tcgctgcgcc ccatcaccga
    81001 gataaagtaa tccccgtcac gggttaaaac gcctttaaac atcggatcgt cattgagcaa
    81061 agtacgcaat ttacgcgcga tggcgatggt gacatttttc tcccgcgtac caccggggcc
    81121 gatagcgcca gggtcctgac cgccgtgtcc ggcatcaata gcgatgataa ttttatcgcc
    81181 attgttagcc gtcgcgcgcg ctgccggacg cgttaccgta ttactgctga taacacccgt
    81241 agtgcggtta ctttccgttt taaacggatt gcgcgccggt tcgctgacgc gcggtgcgac
    81301 aaccgtaggc gtttcaacgc gtttcacaac cacaggcggc ggaggaggcg gtggcggcgc
    81361 atcggcgtta atcgtaaaga cgacagtgta attgctgcca ttctgccgct tcaccgcttc
    81421 ggttttacca ttttcggtaa gatcgaccac cagccgtagc gtttgtgcat ctttaggcgt
    81481 tccggagcga atcgccttca ccagattatt accgctgaac aacaacggca gtccctgaat
    81541 cacgcccgtt tgtttgatat cgagcgccac ggtgcgtttg ctttgatggc taaacgcata
    81601 atcaggatcg ccaataaaac tcaacgttat ccgcgcctgt tggttgccgt tagaaacctg
    81661 aatatcagag agcgtcgcgg cacccacctg cgcgcacagc agcagcaacg tcgctaccaa
    81721 ccaatttctg atgcgataca tcatcccacc acctttcaaa ggttaaccgg ctaaacgtgc
    81781 cagcaacaat tcacccgcag aggaaaccgc actcacgcgc gcctcacggc cttgtgcctg
    81841 ataatcaatg tgtatttcga catccgggtc aggaagaaca cctgtacctt gttgtggcca
    81901 ctccaccagg cagatggcat cgttggcaaa ataatcgcgg atccccataa actccagctc
    81961 ctcgggatcg gcaaggcggt acaaatcaaa gtgataaacc attaaattgt cgagcgtata
    82021 gggttcgacc agcgtataag tggggctttt gacattaccc tgatggccca aagcctgtaa
    82081 aaagccccgg ctaaaggtgg ttttacctgc gcctaaatcg ccatacagat agattacggt
    82141 tgcgccatcg caggctttcg ctacccgctc gcccaggtct aatgttgcct gctcatcagg
    82201 gagcggaatt actcgattca tcatggtttt tatcagtcac ttccgggtta acaatacgct
    82261 gtagcgtgga aaagagatcg gttgccagca tcccgcgcgt tccaaaacgc gccgccagta
    82321 cgtcagctgc cgcaccgtgc gcgacacagc ctgcacaggc tgcatcatac ggcgacagtt
    82381 tttgcccaag caatgcgcca ataataccag agagcacatc gcccatgccg ccgcttgcca
    82441 tgcctgcatt tccggcatca ataatgccta aagcgtcagg atgggcggcg accacggttc
    82501 cggcaccttt cagcaccgct acgccgccat aacgttgtac cagacgtttg gcgcaatgta
    82561 agcggtcact ttcaatttca gcgacggaac agcctaacaa ccgtgcggcc tcgccaggat
    82621 gcggcgtgat cacgcgattg tgacgcttat cgggattgat tgccagcagg ttcaatgcat
    82681 cggcatccca caacatcggt ttgcgaaaat tctcaacttt ttgcagggct tttttccccc
    82741 actcttgctg gcccagaccg ggaccaatca ccaccacatc ggcccattcc aggctttcgg
    82801 taagagagtc catagtcagt tcatgcacca tcaattctgg tcgtgcagtc agcagcggcg
    82861 caatgttctc actgcgggtc agtactcgga ctaaaccagc accagcacgt agcgccgctt
    82921 cccccgtcat acgaatagcc cccgccgtgc cgtgatcgcc accgataatc accagccgcc
    82981 cgtgatcgcc tttatgcgaa gtcgggcgac gcggtttcag ccagtgagaa agttgttctg
    83041 ccgaaaaccg ctgaattttc gtctcctgac ctgccagcca gctatccagc cccagtgagt
    83101 caaaatgcag ttgtcctgta acatcccgcg cttttccagt gagcaagcct ggtttcagcg
    83161 caataaaagt gatggtgtga tcggcgttga tcactgcgcc tggcgtagcg ccggtttcag
    83221 ccagcaggcc ggaagggata tcaaccgcca caatcggcgc aggatgggaa ttagcgtggt
    83281 cgattaactg gctaatggat tcgcggggtg cttgctgcaa gccggtaccg agcagcgcat
    83341 caacaatcag agctaccgac tcgggccaga caatattcga agcatggatc tcgcctcccg
    83401 cgtttaacca tgcctcgcgt gccagcgcgg cctcttccgg caacggttta tcgctctcct
    83461 gggccaacaa cgtgacctca atgccgaccg ctttggccag tcgcgcgacc acgtagccat
    83521 cgccgccgtt attaccatga ccgcacaaca ccagccagtg gcgggcgtca ggatacgccg
    83581 aacgacacac ctggaatgcg gcctcgccag cgcgaagcat cagctcatag agtgtgagcc
    83641 ccagcgcatc tgccgcctcg cgttctccgc ggcggatatc gtcggcgtgc cagacggtgt
    83701 gtggtatact tacggggttt ttcttcattg tatggtccgt catgtcagag cccctcgatc
    83761 tcaatcagtt agcgcaaaaa attaaacagt gggggctgga actgggcttt cagcaggtag
    83821 gtattaccga taccgatctc agcgagtccg agcccaaact gcaagcatgg ctggacaaac
    83881 aataccacgg cgaaatggac tggatggcac gtcacggtat gctgcgcgct cgcccccatg
    83941 agttattgcc cggtacgctg cgcgtgatca gcgtgcggat gaattacctt cctgctaacg
    84001 ccgcatttgc cagcacgctg aaaaacccca aactcggcta tgttagccgt tatgcgctgg
    84061 gccgtgacta tcacaaactt ctgcgcaacc gactcaaaaa gctgggcgag atgattcagc
    84121 aacattgtgt ttcgctgaat tttagaccgt ttgtcgattc tgcgcctatt ctcgagcgcc
    84181 cgttagctga aaaagctggg ctcggctgga caggtaagca ctcacttatc ctcaatcgcg
    84241 aggccggttc gttcttcttt ttaggcgaat tgctggtcga tattccgctg cccgtggatc
    84301 aaccagtcga ggaaggatgc ggcaaatgcg tggcctgtat gacgatttgc ccgaccggtg
    84361 ccatcgtcga gccatatacc gtcgatgctc gccgctgtat ctcttatctc accatcgaac
    84421 tggaaggggc gatcccggaa gagttgcgac cgttaatggg aaaccgtatt tacggttgcg
    84481 atgactgcca gcttatctgc ccgtggaatc gctattcaca actcactaca gaagacgatt
    84541 tcagcccgcg taagccgcta cacgcaccgg aactcattga gttattcgcc tggagcgaag
    84601 agaagttttt aaaagtcacg gaaggttcgg cgattcgccg tataggtcac ctgcgctggc
    84661 tgcgtaatat cgccgtggca ttaggcaatg cgccctggga tgaaacgatt ctggcggcgc
    84721 tcgagagtcg caaaggtgag cacccacttc ttgatgagca catagcgtgg gcgatggcgc
    84781 agcaaatcga gaggcgaaat gcgtgcatag tcgaagtgca attgccgaaa aaacagcgtc
    84841 tggttcgggt aattgaaaaa gggttaccgc gtgacgcctg attcatccac agcttgtgaa
    84901 taaaaataaa aacgcattgc aattcaagac gaataaaatc gtcaagtgat cgactcgaca
    84961 aaacgaaatc ttaattttta aattatctat caataggtta aaaaaatact attcaccgtg
    85021 cgaagtttct ttgaaattaa gattacggcc tgaggctgtg gataactctg tttacaagag
    85081 tttgtcagag acaacaaaaa ccagcaataa cgcatcgcgt cgctgtggat attttattga
    85141 gagaagaatt tggagcgg
//
LOCUS       DAZFAB010000009        81871 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZFAB010000009 DAZFAB010000000
VERSION     DAZFAB010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 81871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 81871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..81871
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.009"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            10..177
                     /gene="yhfL"
                     /locus_tag="ACHJWA_000749"
     CDS             10..177
                     /gene="yhfL"
                     /locus_tag="ACHJWA_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFO4307403.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            473..1810
                     /gene="frlA"
                     /locus_tag="ACHJWA_000750"
     CDS             473..1810
                     /gene="frlA"
                     /locus_tag="ACHJWA_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HFO4307404.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFAAPATTAIGATGSFMALLAGISATSWSYTGMASICYMT
                     GEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPAL
                     GSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSI
                     ILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGL
                     MTPLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
     gene            1831..2853
                     /gene="frlB"
                     /locus_tag="ACHJWA_000751"
     CDS             1831..2853
                     /gene="frlB"
                     /locus_tag="ACHJWA_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFO4307405.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHTIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDARCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPHAEIGKIKNDLKQLPNALGYLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAAISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            2904..3731
                     /gene="frlC"
                     /locus_tag="ACHJWA_000752"
     CDS             2904..3731
                     /gene="frlC"
                     /locus_tag="ACHJWA_000752"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HFO4307406.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYALQALERFRALLPEDE"
     gene            3731..4516
                     /gene="frlD"
                     /locus_tag="ACHJWA_000753"
                     /pseudo
     CDS             3731..4516
                     /gene="frlD"
                     /locus_tag="ACHJWA_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
     gene            4616..5347
                     /gene="frlR"
                     /locus_tag="ACHJWA_000754"
                     /gene_synonym="yhfR"
     CDS             4616..5347
                     /gene="frlR"
                     /locus_tag="ACHJWA_000754"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFO4307407.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERISAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(5478..6482)
                     /gene="trpS"
                     /locus_tag="ACHJWA_000755"
     CDS             complement(5478..6482)
                     /gene="trpS"
                     /locus_tag="ACHJWA_000755"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFO4307408.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGDIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASAHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(6475..7233)
                     /gene="gph"
                     /locus_tag="ACHJWA_000756"
     CDS             complement(6475..7233)
                     /gene="gph"
                     /locus_tag="ACHJWA_000756"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFO4307409.1"
                     /translation="MSKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(7226..7903)
                     /gene="rpe"
                     /locus_tag="ACHJWA_000757"
     CDS             complement(7226..7903)
                     /gene="rpe"
                     /locus_tag="ACHJWA_000757"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFO4307410.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(7921..8757)
                     /gene="dam"
                     /locus_tag="ACHJWA_000758"
     CDS             complement(7921..8757)
                     /gene="dam"
                     /locus_tag="ACHJWA_000758"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO4307411.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(8864..10150)
                     /gene="damX"
                     /locus_tag="ACHJWA_000759"
     CDS             complement(8864..10150)
                     /gene="damX"
                     /locus_tag="ACHJWA_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFO4307412.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(10242..11330)
                     /gene="aroB"
                     /locus_tag="ACHJWA_000760"
     CDS             complement(10242..11330)
                     /gene="aroB"
                     /locus_tag="ACHJWA_000760"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFO4307413.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGTFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(11387..11908)
                     /gene="aroK"
                     /locus_tag="ACHJWA_000761"
     CDS             complement(11387..11908)
                     /gene="aroK"
                     /locus_tag="ACHJWA_000761"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFO4307414.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(12309..13547)
                     /gene="hofQ"
                     /locus_tag="ACHJWA_000762"
     CDS             complement(12309..13547)
                     /gene="hofQ"
                     /locus_tag="ACHJWA_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFO4307415.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRNITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(13459..13863)
                     /gene="hofP"
                     /locus_tag="ACHJWA_000763"
     CDS             complement(13459..13863)
                     /gene="hofP"
                     /locus_tag="ACHJWA_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFO4307416.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(13853..14293)
                     /gene="hofO"
                     /locus_tag="ACHJWA_000764"
     CDS             complement(13853..14293)
                     /gene="hofO"
                     /locus_tag="ACHJWA_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFO4307417.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQHWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVFWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(14277..14816)
                     /gene="hofN"
                     /locus_tag="ACHJWA_000765"
     CDS             complement(14277..14816)
                     /gene="hofN"
                     /locus_tag="ACHJWA_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFO4307418.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTS
                     SAEARVDAVLLQGEQQLARSLQITKPRLLERQQIREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(14800..15594)
                     /gene="hofM"
                     /locus_tag="ACHJWA_000766"
     CDS             complement(14800..15594)
                     /gene="hofM"
                     /locus_tag="ACHJWA_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFO4307419.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDARRLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGWRLGRRTDEPAN"
     gene            15714..18266
                     /gene="mrcA"
                     /locus_tag="ACHJWA_000767"
     CDS             15714..18266
                     /gene="mrcA"
                     /locus_tag="ACHJWA_000767"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFO4307420.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(18431..18991)
                     /gene="nudE"
                     /locus_tag="ACHJWA_000768"
     CDS             complement(18431..18991)
                     /gene="nudE"
                     /locus_tag="ACHJWA_000768"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFO4307421.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVFEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            19311..21446
                     /gene="igaA"
                     /locus_tag="ACHJWA_000769"
     CDS             19311..21446
                     /gene="igaA"
                     /locus_tag="ACHJWA_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFO4307422.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHLVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            21511..22179
                     /gene="yrfG"
                     /locus_tag="ACHJWA_000770"
     CDS             21511..22179
                     /gene="yrfG"
                     /locus_tag="ACHJWA_000770"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFO4307423.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            22190..22591
                     /gene="hslR"
                     /locus_tag="ACHJWA_000771"
     CDS             22190..22591
                     /gene="hslR"
                     /locus_tag="ACHJWA_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFO4307424.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            22616..23494
                     /gene="hslO"
                     /locus_tag="ACHJWA_000772"
     CDS             22616..23494
                     /gene="hslO"
                     /locus_tag="ACHJWA_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFO4307425.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(23557..25281)
                     /gene="yhgE"
                     /locus_tag="ACHJWA_000773"
     CDS             complement(23557..25281)
                     /gene="yhgE"
                     /locus_tag="ACHJWA_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HFO4307426.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLSGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQTDGMTPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWSELFKLVGITFFKTLFFATDWFIYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFIAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRKGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEHSGRYGRAALESLKSDAEYMKDPKRARDLLMALDGEQHLQQQISEKV
                     LAENVLIAPGSGKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGRAERILFAF
                     DDERYIVYGFDPDKKEWQELTMSLLPRDITKEKLLTAAKDGKLGTKPKAWRDLVVDGE
                     RLDVNLNE"
     gene            25660..27282
                     /gene="pckA"
                     /locus_tag="ACHJWA_000774"
     CDS             25660..27282
                     /gene="pckA"
                     /locus_tag="ACHJWA_000774"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFO4307427.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTKQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVSEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(27358..28710)
                     /gene="envZ"
                     /locus_tag="ACHJWA_000775"
     CDS             complement(27358..28710)
                     /gene="envZ"
                     /locus_tag="ACHJWA_000775"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFO4307428.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQSTTKEG
                     "
     gene            complement(28707..29426)
                     /gene="ompR"
                     /locus_tag="ACHJWA_000776"
     CDS             complement(28707..29426)
                     /gene="ompR"
                     /locus_tag="ACHJWA_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFO4307429.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            29654..30130
                     /gene="greB"
                     /locus_tag="ACHJWA_000777"
     CDS             29654..30130
                     /gene="greB"
                     /locus_tag="ACHJWA_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFO4307430.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            30227..32548
                     /gene="yhgF"
                     /locus_tag="ACHJWA_000778"
     CDS             30227..32548
                     /gene="yhgF"
                     /locus_tag="ACHJWA_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFO4307431.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLGYLRELEERRQAILKSISEQGKLTDDLANAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAENEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNDRPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            32986..33213
                     /gene="feoA"
                     /locus_tag="ACHJWA_000779"
     CDS             32986..33213
                     /gene="feoA"
                     /locus_tag="ACHJWA_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFO4307432.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            33230..35551
                     /gene="feoB"
                     /locus_tag="ACHJWA_000780"
     CDS             33230..35551
                     /gene="feoB"
                     /locus_tag="ACHJWA_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFO4307433.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            35551..35787
                     /gene="feoC"
                     /locus_tag="ACHJWA_000781"
     CDS             35551..35787
                     /gene="feoC"
                     /locus_tag="ACHJWA_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFO4307434.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            35990..36868
                     /gene="rpnA"
                     /locus_tag="ACHJWA_000782"
     CDS             35990..36868
                     /gene="rpnA"
                     /locus_tag="ACHJWA_000782"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFO4307435.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDIT
                     VMPDDEIMQHRRMTLLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASQ"
     gene            complement(36895..37665)
                     /gene="bioH"
                     /locus_tag="ACHJWA_000783"
     CDS             complement(36895..37665)
                     /gene="bioH"
                     /locus_tag="ACHJWA_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312282.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFO4307436.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLAEMAEAVLRQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRLPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFRHVLVALKQRV"
     gene            37703..38386
                     /gene="gntX"
                     /locus_tag="ACHJWA_000784"
     CDS             37703..38386
                     /gene="gntX"
                     /locus_tag="ACHJWA_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFO4307437.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSLLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAITRTRAT
                     ATQHFLSARLRRRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            38445..39020
                     /gene="nfuA"
                     /locus_tag="ACHJWA_000785"
     CDS             38445..39020
                     /gene="nfuA"
                     /locus_tag="ACHJWA_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFO4307438.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            39381..40697
                     /gene="gntT"
                     /locus_tag="ACHJWA_000786"
     CDS             39381..40697
                     /gene="gntT"
                     /locus_tag="ACHJWA_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO4307439.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(40742..42826)
                     /gene="malQ"
                     /locus_tag="ACHJWA_000787"
     CDS             complement(40742..42826)
                     /gene="malQ"
                     /locus_tag="ACHJWA_000787"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFO4307440.1"
                     /translation="MESKRLDNAALAAGISPHYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHITGGKAFNLPT
                     KLPEGYHTLTLTQEDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVADQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWQAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLETMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(42836..45229)
                     /gene="malP"
                     /locus_tag="ACHJWA_000788"
     CDS             complement(42836..45229)
                     /gene="malP"
                     /locus_tag="ACHJWA_000788"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFO4307441.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLKKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            45841..48546
                     /gene="malT"
                     /locus_tag="ACHJWA_000789"
     CDS             45841..48546
                     /gene="malT"
                     /locus_tag="ACHJWA_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFO4307442.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            48613..48891
                     /locus_tag="ACHJWA_000790"
     CDS             48613..48891
                     /locus_tag="ACHJWA_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFO4307443.1"
                     /translation="MKPESKEAPINIRAKASQRDLIDMAANLVAKSRTDFMLDAACRE
                     AQDILLDQRLFILDDEQYDAFLAALDAPITAERQAKINALMNRKSPWE"
     gene            48882..49367
                     /locus_tag="ACHJWA_000791"
     CDS             48882..49367
                     /locus_tag="ACHJWA_000791"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO4307444.1"
                     /translation="MGITAPTPLTSEHNLADFCCSDHGMNEWLKKKALKNHSSGLSRV
                     YVICIANTRQVIGYYCLSTGSIQRNLAPGAMRRNAPESLPVVVLGRLAIDQAWAGKGL
                     GVALLKDAVYRTMSIAQQVGVRALIVHALDDSVRNFYLKYAFVPSPFQSLTLLYPITL
                     E"
     gene            complement(49417..50445)
                     /gene="rtcA"
                     /locus_tag="ACHJWA_000792"
     CDS             complement(49417..50445)
                     /gene="rtcA"
                     /locus_tag="ACHJWA_000792"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFO4307445.1"
                     /translation="MKRMIALDGAQGEGGGQIMRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAATEICGATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAASFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGQFTVAHPSCHLLTNIAVVERFLPVRFTLAETDGVTR
                     VMITKLTD"
     gene            complement(50449..51675)
                     /gene="rtcB"
                     /locus_tag="ACHJWA_000793"
     CDS             complement(50449..51675)
                     /gene="rtcB"
                     /locus_tag="ACHJWA_000793"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFO4307446.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDVMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKMFSVEDQIRATVHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            51863..53461
                     /gene="rtcR"
                     /locus_tag="ACHJWA_000794"
     CDS             51863..53461
                     /gene="rtcR"
                     /locus_tag="ACHJWA_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFO4307447.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCICQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVSVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSLYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVADGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHASLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFASSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(53443..54201)
                     /locus_tag="ACHJWA_000795"
     CDS             complement(53443..54201)
                     /locus_tag="ACHJWA_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFO4307448.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDTPPPVSVMQVLTDHHIQLELC"
     gene            complement(54218..55048)
                     /gene="glpG"
                     /locus_tag="ACHJWA_000796"
     CDS             complement(54218..55048)
                     /gene="glpG"
                     /locus_tag="ACHJWA_000796"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFO4307449.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPALKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(55093..55419)
                     /gene="glpE"
                     /locus_tag="ACHJWA_000797"
     CDS             complement(55093..55419)
                     /gene="glpE"
                     /locus_tag="ACHJWA_000797"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFO4307450.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRNNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            55609..57114
                     /gene="glpD"
                     /locus_tag="ACHJWA_000798"
     CDS             55609..57114
                     /gene="glpD"
                     /locus_tag="ACHJWA_000798"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFO4307451.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAVSDLGEDFGHEFYETE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(57329..57931)
                     /locus_tag="ACHJWA_000799"
     CDS             complement(57329..57931)
                     /locus_tag="ACHJWA_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4307452.1"
                     /translation="MMNIIRLYIYPLFLGFVIVPLLVWPTVVALAACVITLSFLGEIL
                     FSIPLMHRRISLLQLQLWLSAEYALFFCAMVGVGWQFARRTPPELKSRLHCWLVFAPV
                     YFWLLLWNIIFYVAPAQIALLENMRSFFLTVVWLPLNFSPFWSQQWIDFVGPISAQLG
                     FALGYYCQWRSKNRSQIRKWSEWGTCLSFVTLGMMAWALR"
     gene            complement(57931..58923)
                     /locus_tag="ACHJWA_000800"
     CDS             complement(57931..58923)
                     /locus_tag="ACHJWA_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO4307453.1"
                     /translation="MNKFTGVLLLGTALLAGCVDREGYYNSVREEESHGLTSLRGQPA
                     LRYSDDWSRWPRVYGATALYPLYASAYYKLVPEPKDKDRTSLAWQAYGLQQTRTAEAY
                     DSLIKGSATVIFVAQPSEGQKKRAEEAGVKLKYTAFAREAFVFIVDINNPVNSLSEHQ
                     VKDIFSGKTSRWNKVGGSDEHIKVWQRPEDSGSQTIMKGLVMQDTPMLPAKKSTVIDL
                     MGGLITEVADYQNTPSSIGYTFHYYVTRMNDNMLKMRKQIKLLAINGVAPTEENIRNG
                     TYPYIVDAYMVTRENPTPETQKFVDWFISQQGQQLVEDVGYVPLYEASPESSGQ"
     gene            complement(58949..60457)
                     /locus_tag="ACHJWA_000801"
     CDS             complement(58949..60457)
                     /locus_tag="ACHJWA_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO4307454.1"
                     /translation="MMPNRKWILTSLIMTFFGIPILAQFLAAVVAMLGVGLTGIIEVC
                     NILITPTIYLLLNVFMLTLGAIIIFFSGRVWAGDSAPENREIAVWRQCFFLLPALLTL
                     VGWIITLHLADYQFRQMGAGWLANLMLPWLGVFLVSLVGGEYWWMVIIPVGAHISFSL
                     GYAWPTRYPLSGTSGLRCRNLLLFLLLLLGIVAGYQAHLYKQQNPGVGVRENIDIRAW
                     RPDKLNNRLTPLRGKPQIQFRQNWPRIDGATAAYPIYASAFYALSVIPEDFHVWEYLE
                     NSRTPDAYNRIVKGDADIIFVAQPSGGQKKRAEESGVTLLYTPFAREAFVFIVNADNP
                     VNSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKKVRMISP
                     QETEVASVMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIRLLAINGITPTAENI
                     RNGKYAYIVDAFMVTRENTTSETQKLVEWFLTPQGQSLVEDVGYVPLYLTME"
     gene            complement(60582..63029)
                     /gene="glgP"
                     /locus_tag="ACHJWA_000802"
     CDS             complement(60582..63029)
                     /gene="glgP"
                     /locus_tag="ACHJWA_000802"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFO4307455.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADLDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(63048..64481)
                     /gene="glgA"
                     /locus_tag="ACHJWA_000803"
     CDS             complement(63048..64481)
                     /gene="glgA"
                     /locus_tag="ACHJWA_000803"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFO4307456.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(64481..65776)
                     /gene="glgC"
                     /locus_tag="ACHJWA_000804"
     CDS             complement(64481..65776)
                     /gene="glgC"
                     /locus_tag="ACHJWA_000804"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFO4307457.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(65794..67767)
                     /gene="glgX"
                     /locus_tag="ACHJWA_000805"
     CDS             complement(65794..67767)
                     /gene="glgX"
                     /locus_tag="ACHJWA_000805"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFO4307458.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALHEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGVGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(67764..69950)
                     /gene="glgB"
                     /locus_tag="ACHJWA_000806"
     CDS             complement(67764..69950)
                     /gene="glgB"
                     /locus_tag="ACHJWA_000806"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFO4307459.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPLNEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(70223..71326)
                     /gene="asd"
                     /locus_tag="ACHJWA_000807"
     CDS             complement(70223..71326)
                     /gene="asd"
                     /locus_tag="ACHJWA_000807"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO4307460.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            71518..72111
                     /gene="yhgN"
                     /locus_tag="ACHJWA_000808"
     CDS             71518..72111
                     /gene="yhgN"
                     /locus_tag="ACHJWA_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFO4307461.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(72157..73452)
                     /locus_tag="ACHJWA_000809"
     CDS             complement(72157..73452)
                     /locus_tag="ACHJWA_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFO4307462.1"
                     /translation="MDADSSNSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLL
                     TSDFSRAAASKYKPLSNSEWGKFALWLKHQRISPAELLVPQPQEKLTGWSDPRISQER
                     ILGLLARGHSLALAVDKWQRAGLWILTRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
                     QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEAGGTA
                     VGVLADSLLKTSTLVKWREGLIAGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAM
                     VVRAGMTGGTITGAMEALKHQWLPVQVKPNQDMQSANSRLVENGASWSAEQAENVTIR
                     LPDVPGLMYDRALRNAQPELFSLHEDDANYAVMPAYTPVDFYQLFVAELAILAKESIS
                     IERLASCTGLTIEQISVWLNRAEEEGRVIRLGEGHYQFR"
     gene            complement(73359..75483)
                     /locus_tag="ACHJWA_000810"
                     /pseudo
     CDS             complement(73359..75483)
                     /locus_tag="ACHJWA_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
     gene            complement(75623..76963)
                     /gene="gntU"
                     /locus_tag="ACHJWA_000811"
     CDS             complement(75623..76963)
                     /gene="gntU"
                     /locus_tag="ACHJWA_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO4307463.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHLFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(76967..77494)
                     /gene="gntK"
                     /locus_tag="ACHJWA_000812"
     CDS             complement(76967..77494)
                     /gene="gntK"
                     /locus_tag="ACHJWA_000812"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFO4307464.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(77633..78628)
                     /gene="gntR"
                     /locus_tag="ACHJWA_000813"
     CDS             complement(77633..78628)
                     /gene="gntR"
                     /locus_tag="ACHJWA_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFO4307465.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(78852..79547)
                     /gene="yhhW"
                     /locus_tag="ACHJWA_000814"
     CDS             complement(78852..79547)
                     /gene="yhhW"
                     /locus_tag="ACHJWA_000814"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFO4307466.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASISDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(79670..80707)
                     /gene="yhhX"
                     /locus_tag="ACHJWA_000815"
     CDS             complement(79670..80707)
                     /gene="yhhX"
                     /locus_tag="ACHJWA_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO4307467.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLNEVLNDPDVKLVVVCTHADSHFEYAKRAMEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            81041..81529
                     /gene="yhhY"
                     /locus_tag="ACHJWA_000816"
     CDS             81041..81529
                     /gene="yhhY"
                     /locus_tag="ACHJWA_000816"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO4307468.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYYNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDARWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYFMAR
                     VK"
     gene            81740..>81871
                     /locus_tag="ACHJWA_000817"
     CDS             81740..>81871
                     /locus_tag="ACHJWA_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4307469.1"
                     /translation="MGTAAISHLRYDLNKYALSLRKTATLASTFFIESPLVRFEYLQE
                     "
BASE COUNT        19774 a        22215 c        20507 g        19375 t
ORIGIN      
        1 ggaatgtaaa tgaacaaatt tattaaagtt gcactggtag gtgcagtact ggctacgtta
       61 actgcatgta ctggtcatat tgaaaaccgt gataagaact gctcttacga ctacctgctg
      121 cacccagcaa tttctatttc taaaatcatt ggcggttgcg gtcctactgc acagtaagtc
      181 tctgacagaa atcagctaac gctgatttaa ttgcctgacg ccctcgggat tacccgaggg
      241 cgtatttgtt tctatatcct tcttgctccc cacccgtaaa aatattttca ctttaatttc
      301 aattggataa ccacattcat cagccacagc gcgatcctgt gcgaaattat gtgatcttcc
      361 tccacattac ataacatcat atgttgttat attcatcatg cattgtcatg tcacctttta
      421 aatgactgca aactctcccc tacaacgtgt tggacaagaa aaaggtatcg ctatgggaag
      481 ccaggaactc caacgcaagc tcggattttg ggccgttctt gcaatcgccg tcgggacaac
      541 cgtcggctcc ggtatttttg tatctgtggg tgaagtggca aaagcggcgg gcacgccgtg
      601 gctgacggtg ctcgcatttg tcatcggcgg gttaattgtg atcccgcaaa tgtgcgtata
      661 tgcggaacta tccaccgctt atccggaaaa tggcgcagat tatgtttatc tgaaaaatgc
      721 cggaagccga ccgctggctt tcctctccgg ctgggccagc ttctgggcca acgatgcgcc
      781 gtcattgtcg attatggcgc tggcgattgt cagcaatctt ggctttttaa cgcctatcga
      841 tccgttgctc ggtaaattta tcgccgccgg attaattatc gcctttatgc tgctgcacct
      901 gcgctccgtt gaaggcggcg cagcgtttca gacgctaatt accatcgcca aaattatccc
      961 gttcactatc gtcattggtc ttgggatctt ctggtttaaa gcggagaatt ttgccgcccc
     1021 tgctaccact gcgattggcg caacgggcag ctttatggcg ctgctggcgg ggatctctgc
     1081 caccagttgg tcgtataccg gcatggcctc tatctgttat atgaccggcg aaattaaaaa
     1141 ccccggcaaa accatgccac gagcgctgat tggttcctgt ctgctggttc tggtgctcta
     1201 caccctgctg gcgctggtga tttccggcct gatgcccttc gacaaactcg ccaattctga
     1261 aacgccgatt tccgacgccc tgacctggat ccccgcactc ggcagcaccg ctgggatctt
     1321 tgttgccatc acggcgatga tcgtcattct tggttcgctt tccagctgcg tgatgtacca
     1381 gccgcggctg gaatacgcga tggcgaaaga caacctgttc ttcaaatgct tcggccatgt
     1441 gcatccgaaa tacaacacgc cggatgtctc catcattttg caaggggcgc tggggatctt
     1501 cttcatcttc gtttccgatc tcaccagcct gctgggctat ttcaccctgg taatgtgttt
     1561 caaaaatacc ctcaccttcg gctccatcat ctggtgtcgt aaacgcgacg attacaaacc
     1621 gctgtggcgt actccggctt tcgggctgat gacccccctc gccattgcgt caagcctcat
     1681 tctggtcgcc tcaacctttg tctgggcacc gattcccggc cttatctgcg ccgtcatcgt
     1741 tattgccact ggtctgcctg cttacgcctt ctgggcgaag cgtagccgcc agctcaacgc
     1801 tttgtcgtaa tgttatctgg agaaaataaa atgttggata ttgataaaag cactgtggac
     1861 tttctggtca ccgaaaatat ggttcaggaa gtggaaaaag ttctcagcca tgacgttccg
     1921 ctggtgcaca ccatcgtgga agagatggtg aagcgcgaca tcgatcgtat ttatttcgtt
     1981 gcctgcggat cgccactcaa cgcggcgcaa acggcgaaac atctggcaga tcgcttttcc
     2041 gatcttcagg tttacgccat ttccggctgg gagttctgcg ataacacccc atatcgcctc
     2101 gacgctcgtt gcgcggtgat tggcgtttct gactacggta aaaccgaaga ggtaatcaaa
     2161 gcgctggagc tgggccgggc ctgcggcgca ctcactgcgg cgttcaccaa acgcgcggat
     2221 agcccgatta cctcggcggc ggaatttagc attgattatc aggccgactg tatctgggaa
     2281 attcatctgc tgctctgcta cagcgtggtg ctggagatga tcacccgcct cgcgccgcac
     2341 gcggaaatcg gcaagatcaa aaacgatctc aaacagctgc cgaatgcgct cggttatctg
     2401 gtacgcactt gggaagaaaa aggccgccag cttggtgaac tggccagcca gtggccgatg
     2461 atttacacgg ttgctgcagg cccgctgcgc ccgctgggtt acaaagaagg catcgtgacg
     2521 ctgatggaat ttacctggac gcacggctgt gtgattgaga gcggtgagtt ccgtcacggc
     2581 ccgctggaga ttgtcgaacc gggcgttccg ttcctgttcc tgctcggcaa tgatgaaagt
     2641 cgccacacca ccgaacgcgc cattaacttt gttaaacagc gtactgacaa cgtgatcgtc
     2701 atcgattacg ccgcaatttc gcaaggactg cacccgtggc tggcaccgtt cctgatgttc
     2761 gtgccaatgg agtggctctg ctactacctg tctatttaca aagatcacaa cccggatgaa
     2821 cgccgctatt acggtggtct ggtggaatat taatcctctc tcccggcccg gcaacgggcc
     2881 ggatttacgc aaggagttac ccgatgaaaa caggtatgtt tacctgcggc caccagcggc
     2941 tgcctattga acatgcattc cgtgatgcaa gcgagctggg ttacgatggc atcgaaattt
     3001 ggggcggccg cccgcacgcg ttcgcgccgg acttaaaagc gggcggcatc aaacaaatca
     3061 aggcgctggc gcagacgtat cagatgccga ttatcggtta cacgccagaa accaacggct
     3121 atccgtataa catgatgctg ggcgatgaac atatgcgtcg tgaaagcctg gacatgatca
     3181 agctggcgat ggatatggca aaagagatga acgcgggtta tacgctgatt tctgcggccc
     3241 acgcgggcta tctcacgccg cctaacgtta tctgggggcg gctggcagag aacttaagcg
     3301 aactgtgtga gtacgcggaa aacatcggca tggatctgat cctcgaaccg ttaacgccgt
     3361 atgaatcgaa cgtcgtgtgt aatgctaatg atgtgcttca tgcgctggcg ctggtgcctt
     3421 cgccgcgcct gttcagcatg gtcgacattt gcgcgccgta tgtccaggcg gaaccggtga
     3481 tgagttattt cgacaaactg ggcgataaat tacgtcatct gcatattgtc gacagcgacg
     3541 gggccagcga cacgcattac attcccggcg aaggcaaaat gccgctgcgg gaactgatgc
     3601 gcgatattat tgatcggggc tatgagggat actgcacagt ggaactggta acgatgtata
     3661 tgaacgagcc cagactttac gccctccagg cgctggaacg ctttcgcgcg ctgctgccgg
     3721 aggatgagtg atgaaaaccc tggcgacaat cggcgataac tgcgtcgata tctacccgca
     3781 actgaataaa gcgttttctg gcggtaatgc ggtgaacgtg gcggtgtact gcactcgcta
     3841 cggtataaag ccgggatgca ttacctgggt tggtgacgat gactacggca caaagcttaa
     3901 gcaggatctc gccagcatgg gcgtcgatat cagccatgtc catacgaaac acggcgttac
     3961 cgcacaaact caggtggaac tgcacgacaa tgatcgcgtt tttggcgact acaccgaagg
     4021 cgtgatggcc gactttgccc tgagtgaaga ggattacgcc tagctggcgc agtatgacat
     4081 tgtgcacgcg gcaatctggg gacatgcgga agacgcattc ccacagctgc acgctgcggg
     4141 caaacttacc gctttcgacg tctccgacaa gtgggacagc ccgctctggc agacactggt
     4201 gccgcatctc gattttgcct ttgcctccgc accgcaagaa gacgaagcgc tgcgtctgaa
     4261 gatgaaagcg attgttgccc gtggcgcagg cgtggtgatt gtcacgttgg gtgaaaacgg
     4321 cagtattgcc tgggatggcg tgcagttctg gcgtcaggct cctgaacagg tgacggttat
     4381 cgacaccatg ggtgccggag attcgttcat tgccggattc ctttgcggct ggtctgcggg
     4441 gatgacatta ccgcaggcga tggcgcaggg aacggcgtgc gcggcacaaa ctattcagta
     4501 ccacggtgcc tggtaggcat aacgttggcg tgagcatctt cacgccaacg tgctgttact
     4561 tgccggaaaa cgaccctata atccgtgtaa ttcattcttt atttcagggt cgattatgtc
     4621 agctacggac cgctactctc atcaactcct ctacgctacc gtccgccagc ggctgctgga
     4681 tgatatcgcg cagggggttt accaggccgg gcaacagatc cctaccgaaa atgagctttg
     4741 tacacaatat aacgtcagcc gcattaccat tcgcaaagcc atcagcgact tagtggcaga
     4801 cggcgtactg atccgctggc agggcaaagg cacctttgta caaagccaga aggtcgaaaa
     4861 cgccctgctg accgtcagtg gttttaccga ttttggcgtc tcacaaggca aggcgacgaa
     4921 agagaaagtg atcgagcaag aacggatcag cgccgcgccg ttttgcgaaa agctcaatat
     4981 ccccggtaac agcgaagtgt tccatctttg ccgggtgatg tatctcgata aagagccgct
     5041 atttattgat agttcgtgga tcccgctgtc gcgttatcct gactttgatg agatttacgt
     5101 cgaaggaagc tccacctatc agttatttca ggagcgtttt gacacgcgag tggtcagcga
     5161 caaaaagacc atcgatatct ttgccgccac ccgcccgcag gcaaaatggc tgaaatgcga
     5221 actgggcgaa ccgttgtttc gcatcagcaa aatcgccttt gatcagaatg acaaaccggt
     5281 gcacgtctcc gaactcttct gccgcgccaa ccgcatcacc ttaacaattg ataataaaag
     5341 acattaaccg taggccggat aagatgcacc agcatcgcat ccggcgatgc cggcgcactg
     5401 aattttacat cccgcacgtt cccctcaccc taaccctctc cccaaagggg cgaggggacc
     5461 gtccgagcgt tttgatatta cggcttcgcc acaaaaccaa tcgcttcgta caccgctttt
     5521 agcgtacggg aagcgtgcgc gctggctttt tctgcgccgt ctttcatcac ctgttgcagg
     5581 aaggcttcat cgttgcggaa acggtggtaa cgttcctgca attcagtcag cataccggaa
     5641 acggcatcag ccacttcgcc tttcagatga ccatacatct tgccttcgaa ctgtttttcc
     5701 agttccggga tgctctggcc cgttaccgca gaaaggatat ccagcaggtt ggaaacgccc
     5761 gctttgttct gcacatcgta gcgaactacc ggcggctcgt cggagtcggt gaccgcgcgt
     5821 ttgattttct tcactaccga tttcggatct tccagcaggc caataacgtt attgcggtta
     5881 tcgtcagact tggacatctt cttggtcggc tccagcagcg acattacgcg cgcgccggat
     5941 ttcggaataa acggctccgg caccttaaag atatcgccgt acagcgcgtt gaaacgctgg
     6001 gcgatatcac ggctcagttc caggtgctgt ttctggtctt cacccaccgg taccagatta
     6061 gtttgataca gcaggatgtc cgctgccatc agcaccggat agtcaaacag accagcgttg
     6121 atgttctcgg cataacgcgc agatttatct ttaaactgcg tcatgcggct cagttcgccg
     6181 aagtaggtat agcagttcag tgcccagcct aactgcgcat gttccggtac gtgggactga
     6241 acaaaaatgg tgcttttctc aggatcgata ccacaagcca gatacaaggc cagcgtatcc
     6301 agcgtcgctt tacgcagctt ctgtgcatcc tggcgcacgg tgatcgcgtg ttggtcaacg
     6361 atacagtaaa tgcaatggta gtcatcctgc atgtttaccc actgacgcag cgcacccatg
     6421 tagttaccaa tagtcaattc acctgagggc tgtgcgccac taaaaacgat gggcttagtc
     6481 atttttcgat tcctgatttt cgctatgcgg aagccctaat gcgggcagaa ggtcatttat
     6541 agactgataa attacatcag gctggctgag atcgatagcc tcgccgtagt tatatccgta
     6601 ggttaagcca actgatgggc aacctgccgc ttttgctgcc tgaatatcat tgcgtgagtc
     6661 gccaacaaac agcatctgtt gtggagcaat tcccatccgc tcagccacca gtaacagcgg
     6721 gtccggatgc ggttttttgt tttgcacatc atcaccacca atcaccacgc tgaagtattt
     6781 ggcgatatct aaggcttcga gcagcggcgc gacgaacggc gtcggtttgt tggtgaccag
     6841 gcctagcggc aggcctttag cctgcaacgc gcccaacgta tcggcaacgt gcgggaacaa
     6901 aaacgtcccc tcttcggcaa cctcgccata gtaacgatcg aacagtttac gcagaatacg
     6961 tacctgttct tctgccggaa tgtcgtcatc aacgggcggt ttacccattg ttttacgcag
     7021 agtcgcacgt tcctgacgcg cccaggtcaa tgcgcgctcc atcagaacat ctgcgccgtt
     7081 accaatccag gtaataacgc gttcttcacc tgcgacgggc aactccagcg catacagcgc
     7141 catatctacc gcagcagcaa gaccgggagc actgtccacc agcgtaccgt cgagatcaaa
     7201 agcgacgccg cgaatatctt caaacttact catgacttac ctttgccagt tcactgcgca
     7261 tttcatcaat gacttttttg tagtctggct ggtcgaagat tgccgaaccg gcaacgaaca
     7321 tatccgcgcc cgccgcggcg atttcgccaa tgttgttcac cttcacgcca ccgtccactt
     7381 ccagacgaat gtcaaagcca gactcgtcga tacgacgacg tacttcgcgc agtttatcca
     7441 gtgtttgagg aatgaaagat tgaccgccga aaccagggtt gacggacatc agcaggatca
     7501 catccagctt atccatcacg taatccagat agctcagagg tgtcgccggg ttaaatacca
     7561 gacccgcttt acagccattt tctttaatca gttgcagcgt gcggtcaaca tgctcggagg
     7621 cttctggatg aaaggtaatg atgctggcac cagcggcagc gaaatcaggc acaatgcgat
     7681 cgaccggttt caccatcagg tgtacgtcga taggggcggt aatgccgtag ttacgcaagg
     7741 atttcagcac cattggccca atcgtcagat tgggaacgta gtggttatcc atgacgtcaa
     7801 aatgcacgac atcagcgcca gctgccaggg cttttgcggt atcttcaccc aggcgggcaa
     7861 aatcagccga cagaattgag ggggcaatca aatactgttt catccgcttc tccttgagaa
     7921 ttattttttc gcgggtgaaa cgactcctgg tttgtacaaa gccagcagtt cgtccacctt
     7981 tttacgtgtg ccgccgttgc tgcttatact gcgtcgaact ttgacgacat gcaattttgc
     8041 gcgctgatac cactcacgcg ttaacatcgt atcgtgattg gagatcagca caggaatatg
     8101 gcgctcaacc agaccttcgg cgatctccgc cagatgcgct tgttgttcaa gcgtaaaact
     8161 gtttgtgtga tacgccgtaa agttggcggt cgcagacagc ggtgcataag gtggatcgca
     8221 atagacgaca gatgcatcat ctgcgcgcgc catgctatcg gcgtaagact cacaatagaa
     8281 aaaggcattc tgcgcttttt cagcgaagtg atacaactct gcttccggga aatagggttt
     8341 tttgtagcgg ccaaacggca cattaaactc accgcgcaga ttgtaacgac acaggccgtt
     8401 gtaaccgtag cggttcaaat ataaaaacag taccgcccga cggaacggat cctggctttt
     8461 gttgaactct tcgcggaact gatagtaaac ctcggcgcaa tttgtttcgg gaacaaacag
     8521 ctcgcgtgcg gcctgtacgt actcatcagt acgcatcttc acaatgttat agagactgat
     8581 caggtcgcta ttgatatcgg caaggatata acgagaaaag tcggtgttga gaaacaccga
     8641 cccggcacct acaaaaggct caaccagaca ttcgcccttg ggcaaatgcc gtttaatatc
     8701 atcaagcagg ggatacttgc cccctgccca cttcaaaaaa gcgcgatttt tcttcatgct
     8761 gactaactaa ttacaccttc tccggctgtg gagaaagctc cgacagcatc cagcgcttca
     8821 ggcaactccc gcaacgataa accattgcgg gagataacct tgattacttc agatcggcct
     8881 gtacctgacg cagcggtttc gcccacgggt ttttggcctg aacatctgct ggcaatgtag
     8941 ataccgcttt tttcgcctct tctttcgaag cgtacacgcc agaaaccagg acataccacg
     9001 gctgaccatt acgcgtcgtt tcatagacaa cgtagttttt cagattctct ttcttcgccc
     9061 aaccgttcag gttgtcgtag ttagaggaac tgctcagctg cagagtgtaa tggctggacg
     9121 gtgccgattt caacgaaccc acattacctg cggtcttccc tccagcggct ggtgttgccg
     9181 tggtttgcgc cggggatgcc gtctgtactg gagccgtagt cgccgtctct tttggtgctg
     9241 gcgtagaagt cgcagccggt gccttcgtgc tcgccacagg agcagctggt tcagtacgct
     9301 tcggcgtctg tgctaccggc ttcggttccg ttttcacggt tgcttgcggt tttttcggtt
     9361 caatcaccgc ctgctgacga gctggacgcg tagtggccgg acgttcagca gtttgcgttt
     9421 tcgccgtgtc acgcgatgca ttgccattgc gtactggcgc gacagttgct ggttcagtcg
     9481 gcaatgtaga attgaccgcc acattgttca actgttgctg attttgtggc tgggtcagcg
     9541 cattgttcag gtcaccctgc acttcaacac gttgttgacc atccgttgcc gccggggttt
     9601 gcccttgagt cggcgtagaa gagatcggtg gcagagaaac atcctgctga gtattttccg
     9661 cagacgtggt tcccggcgct ggctgcacgc cattcgcctg atcggtcgca ttgccagcaa
     9721 gatcaatact cttctcgcca gacgcggttt gatcgctgga agtggtcgag ggggctttta
     9781 gcgcagaacc gataccgatg atcaacagca gtagaaccag aatgccgacg cccatcatca
     9841 tatactgacg agaagcgggt ttactggctg cttttttgcg cttacgcgga cgacgctcta
     9901 cgcgctcttc atccacggtt tcatcttcgg attcgtcaat ttcttcttcg atttccggtt
     9961 cctcattgcg ctctttttgc gcacgagtcg gacggcgatc gtcagtgtca tcaagttcaa
    10021 tatcatcaaa attgatctgc ggttcgccac gttcagtacg ctcagaacgt tcagaagatt
    10081 gacgagaacg accagtacga cgatcgctgg gatcgggttt cagctcgtct tctggtttga
    10141 attcatccat ttaacacccc actaaaaggt taatgcttac cacgttgcaa ttaacctgaa
    10201 gctaatagac cgcttgataa gcggcctgac ctttcttgtt gttacgctga ttgacaatcg
    10261 gcaatggcgt taagaacaag ctcgtgcgaa acgccgctgc gaacttcact cttaccaatt
    10321 gccaacggaa gaattaagcg catctctccc gcaaggactt tcttgtcacg cagcatatgc
    10381 ggtaaatacg cctgcgcgga catttcgcgc ggcccattga ccggtaaccc agcccgcgtg
    10441 agcagagtta taatacgctg cgtttcggca gaactaaact gcccgagacg ttccgacgtc
    10501 cgcgccgcca tcaccatacc cgcagcgacc gcttcaccat gtaaccaatt gccatacccc
    10561 atttcagctt caatggcatg accaaaggtg tgtcccagat tcagtaaagc acgtaacccg
    10621 gtttcgcgct cgtcggcggc gacaacttct gccttcagtt cacaacaacg gcgaatacag
    10681 tacgccattg ccggaccgtc cagacgcaac aacgcatcca gattctcttc cagccagtta
    10741 aaaaacgtac cgtcaagaat aatgccgtat ttgatgactt ctgccagccc cgacgctaac
    10801 tcacgcgggg gaagcgtttt cagacagtcg agatccacca ccactgaagc aggctggtag
    10861 aacgcgccaa tcatgttttt accgagggga tggttgaccg cagttttgcc gccaacggag
    10921 gaatcgacct gcgacagtaa cgtcgtcggg acttgaatga aacgaacacc gcgctgataa
    10981 ctcgccgccg cgaagccggt cagatcgccc actacgccgc cgccaagcgc caccagcgta
    11041 gtatcgcgac catgcggctt ttgtaacaac gccgtaaaga cggtatcgag tacagccagg
    11101 cttttatact gctcgccgtc aggaaggata acgctatcga cgttaacacc cgcctgttca
    11161 agtacgccgc ggaccttatc gagatacaga ggagccaggg tttcgttggt gaccaacatg
    11221 acctgctcgc ccgatttcag cggtaagaat gaagctggtt cattaaacaa accagatgcg
    11281 atggtaattg ggtaactacg ttccccgaga gtaacgacaa tcctctccat aacgcgacat
    11341 ccaccttaat tactgtaccc gcagacgagt gtatataaag ccagaattag ttgctttcca
    11401 gcatgtgaat aatctggttt gcaaccactt tagcgctttg atcatcagta cgaatggtca
    11461 cgtcggcaat ctcttcatac agcggattgc gttcattggc caacgcttcc agaacttcac
    11521 gcggcggtgt ttcaacgtgc agcaacgggc gttttttatc acgctgcgtg cgtgcaagtt
    11581 gcttttcgat cgtcgtttca agataaacga caacgccacg agcggaaaga cggttacgcg
    11641 tttcacggga tttcacagag ccgccgccag tagccagcac aataccctgt ttctcggtca
    11701 actcattgat gaccttttct tcgcgatcgc ggaagccttc ttcgccttct aaatcgaaaa
    11761 cccagcccac atcagctccg gttcgtttct caatctcttg atcggaatcg taaaattcca
    11821 tattgagttg ttgagctaac tggcgcccaa tagtgctttt tccggcaccc ataggcccaa
    11881 ccagaaagat attgcgtttc tctgccattt tttcggtact actaagacta ttcgttaatg
    11941 ataaacccgc ttcgctcaga gagctccgca ggacatgaac tgaaacctca taagatattg
    12001 cgagagtcag actgaaaatt atctcaatac tccagcgggt ttggcaactg aataaatcac
    12061 ccatccctct gcatatctgg tcgctgcaag cgcgctgcct tgctaccacc gctctggcga
    12121 taaatcaccg ggtaagatta gcgtaaaaaa gacagcaaaa tgccgcctga atgataaatc
    12181 atcatcatgg aaggcaggca gcgaagagca ccaactctca aatcggtact ccttgtatgc
    12241 taaatacctg cgcgcgtcaa atagatgaaa cacgttcagc gtaaaaacat taccgctttt
    12301 acggctgttt actcactgga aaccagtcgt ggcgtgataa acaccactaa ctcgcgtcgt
    12361 tcatcttctt ttccgtcatg acgaaataat tgcccgaacc agggaatgtc gccaagcaac
    12421 ggcacgctat cctgacccga tttattttta cgggtaaaaa tgccgcccag cgccaacgtt
    12481 tctccgcttt tgacctcgac ctgcgtttcg atctcctgct tatcaatcgc cagcacttcg
    12541 ccatcggcct gctgtagcac ctgccccgga acgttctggc tgatgtgtaa tttcagccgg
    12601 atgcgacctt tttgtaacac cgtgggcgtg acctccatcc ccaggacggc ctctttaaat
    12661 tccaccgacg tcgcgccact ttccccgctg gaaacctgat atggaatttc gctcccctgt
    12721 ttaatgctgg caggctgaag atgtgaggcc agcagacgcg gactggcgat aatatccagc
    12781 tgctgttttt gttcgagcgc ggaaagctca agatccagca aacgtccgtt gatgcgccca
    12841 atgttaaaac cgacatgcgt tgtcgccgtc gctacggaga ggtcgctacc aagcgtggtg
    12901 acttgcccaa cgccaccagc gtgttgcgca tcggccagcg tccatttcac gcctaactca
    12961 cgcaaacttt tttcattaat ggtgacaata tgcgccgaca gctcaacctg cccgaccggc
    13021 agatccattt gcgctaccca ctgttcaagg gcgcttaacg ccgttttgtt atctcgtagc
    13081 aaaaggcgat tggtgcgttt atcgacggtc atactccctt tggcactcag tagcttctcc
    13141 ccagctttcg ccagctctcc ggcgtcggcg tattgcagag taatattgcg attttccagc
    13201 ggcagatttg cctgcgcccg cgcctgctcc gcttcctgac gggcgatatt gtcattctgc
    13261 caggcaatgg aatgcactga gagaatgttg ccctcctggc gcgttatcag tccggcgctt
    13321 ttcactacag tttgtagtgc ctgcttccag ggaacatctg ttagatgtaa cgacaccgta
    13381 ccgctgacgt ctggcgacac caccaggttc aacttctcct gttcagccag cgcctgcaac
    13441 acctgagcta ccggaacgtc atccaccatc agcgtcactt tttgcggctt tgccgcctgt
    13501 acgccgggta tcagcatcaa cagtagtgcg gctatccatt gcttcatttg tatctccttg
    13561 ccgttgccac aaccattgtg gcggttcgca gtttgtcccg gtacccagcg ttagtacgtc
    13621 tggcgtcagc tgtaaaattg tccagccgtt ttccagcaca tcgttttgct gcacccgtcg
    13681 ccatttcttt tgcccgtctt ttattacacc gatgatgcgc tcgcctcgcc ctaccatccc
    13741 ctgatagcgc cactggctaa gttcgctaat ccggcataga tcttccggcg gtttaaaagg
    13801 gtcacgcata ccggttaaaa ggcacaatgc aatacctgcc aacaaccagc gtttaaccct
    13861 catgcggcgt ctccagttgt agggtgaaca aaagatcatc accttccacg cttaatgaaa
    13921 aacggctcac gctgacgttg cgctctgcca gccgtgtaaa tgccgacggc actgcttccc
    13981 acagcgtttt caacgccaac tcgcctccct gcgcggatgg atgccagaaa accagttgtg
    14041 cgccgggcaa ctgaaaatcc agtggcgaaa agggcagcgt tttttcttcg ctgaagggag
    14101 tggtgtcaac caggcgatac agtgtggccc aatgctgatg atgacttgcc cgtagtcgaa
    14161 ttaatgcgtc gcgctcttca tggtgtgtcg atgacagaaa aatgagcgta actaacatca
    14221 gcaaccagaa tgcccagcaa aactggcgga ggcggggtga tgtggcgaac caccagtcaa
    14281 agaacatgtt catcgctaac cttccttgtt aactgatact caaattgcca gcgtccctgc
    14341 gcatcctgct gcgtggcccc ccgctgattg agatgaaaag atgcatcctg gcggagtgac
    14401 gtttctagtg cgtttaacgc ggtaatgctt gttgtaagcc ccttgatctc cagcgttccc
    14461 tgctgccagc ttatcgttgt cagccaggcg tgctcgggta aaagcgccgc cagtgcttcc
    14521 agcgcagatt gccagtcgcg ggtaaattgt cgctggcgct gcctttgcaa acgctgctca
    14581 cgaatttgtt ggcgctccag caaacgtggt ttggttatct gtaagctgcg ggcgagttgt
    14641 tgttccccct gaagcaaaac ggcgtctacg cgagcttcgg cgctgcttgt cagacgcagt
    14701 attagcgtta tcccgacggc cagcagcaga ggcgcaacga acatcagcaa ccagaaacga
    14761 agaaaagcgg tccggcgttg ctgtcgccag ggcaaaaaat taattggcgg gttcatcagt
    14821 acgccttccc aagcgccagc ccagcgcgat ggcaaagtct ccacctggcg gcggtaacgg
    14881 cggctgacga acagaaacgg cctcccaagg atcaaatccg ccttcgccac atatcgcgac
    14941 gctttcggga tcaacggata acgctgccgc cagctccttc gcgctagtca tccccaccgc
    15001 cagtttgcgc ccccagcgat agcgcgtcgc ccacagccac tgttcgttat cacgccaggc
    15061 cagacattgc tgatgagaag gtaaaaaagg caggaatcgc tgtaatgcac tggcatccgg
    15121 ggtgatcgca ctaacatgaa cacgcaacct ttctgccaaa gtaagcagcg ttgccagctc
    15181 tttgctttgc gcggcagtca cgttataggc gggactgagt gagtcttcgc tgtaatcgaa
    15241 gcgcagggag tccggatcca tatccagctc gcgagccatc gtcccggaca gccaggccgt
    15301 ttgctcccgc tcaccgaggg acatcgacgg gcgcggaaat gaccgctgta atgtgcgact
    15361 ggcgggaaac gccaacatga tgtgatggcg ctgcggcagt tcgcgactcc acggtaacaa
    15421 cgttttagcc agccgccgcg catcaacaat ccgcccatct ttgataatgt cgtgcgccag
    15481 cggcaaccgc caccagcgtt gcaaaaagca ttcttttgcg ccccgcacaa tagcaaccgc
    15541 taccgcttct tgctgttgta aatgcaaacc aatttgccag atcttaaatg ccattgtgat
    15601 gatctcctta tcacccgtca ctctgacggg tatatcaatg cgtctggctt gcctttatac
    15661 taccgcgcgt ttgtttataa actgcccaaa tgaaactaaa tgggaaattt ccagtgaagt
    15721 tcgtaaagta ttttttgatc cttgcagtct gttgcattct gctgggagca ggctcgattt
    15781 atggcctata ccgctacatc gagccacaac tgccggatgt ggcgacatta aaagatgttc
    15841 gcctgcaaat tccgatgcag atttacagcg ccgatggcga gctgattgct caatacggtg
    15901 agaaacgtcg tattccggtt acgttggatc aaatcccgcc ggagatggtg aaagccttta
    15961 tcgcgacgga agacagccgc ttctacgagc atcacggcgt tgacccggtg gggatcttcc
    16021 gtgcggcaag tgtggcgctg ttctccggcc acgcgtcaca aggggcaagt accattaccc
    16081 agcagctggc gagaaacttc ttcctcagtc cagaacgcac gctgatgcgt aagattaagg
    16141 aagtcttcct cgcgattcgc attgaacagt tgctgacgaa agacgagatc ctcgagcttt
    16201 atctgaacaa gatttacctt ggttaccgcg cctatggtgt cggtgctgcg gcacaagtct
    16261 atttcggaaa aacggtcgac caactgacgc tgaacgaaat ggcggtgata gccgggctgc
    16321 cgaaagcgcc ttccaccttc aacccgctct actcgatgga tcgtgccgtc gcgcggcgta
    16381 acgtcgtgct atcgcggatg ctggatgaag gatatatcac ccagcaacag ttcgatcaga
    16441 cacgcactga ggcgattaac gctaactatc acgcgccgga gattgctttt tctgcgccgt
    16501 acctgagcga aatggtgcgc caggagatgt ataaccgtta tggcgaaagt gcctatgaag
    16561 acggttatcg catttacacc accatcaccc gcaaagtgca gcaggccgcg cagcaggcgg
    16621 tacgtaataa cgtgctggac tacgacatgc gccacggcta tcgcggcccg gcaaatgtgc
    16681 tgtggaaagt gggcgagtcg gcgtgggata acaacaagat taccgatacg ctgaaggcgc
    16741 tgccgaccta tggtccgctg ctgcctgccg cagtcaccag cgccaatcct caggaagcga
    16801 cggcaatgct ggcggacggg tcgaccgtcg cattgagtat ggaaggcgtt cgctgggcgc
    16861 gtccttaccg ttcggatact cagcagggac cgacgccgcg taaagtgacc gatgttctgc
    16921 aaacgggtca gcaaatctgg gttcgtcagg ttggcgatgc atggtggctg gcacaagtgc
    16981 cggaagtgaa ctcggcactg gtgtcgatca atccgcaaaa tggtgccgtt atggcgctgg
    17041 tcggtggctt tgatttcaat cagagcaagt ttaaccgcgc cacccaggca ctgcgtcagg
    17101 tgggttccaa catcaaaccg ttcctctaca ccgcggcgat ggataaaggt ctgacgctgg
    17161 caagtatgtt gaacgatgtg ccaatttctc gctgggacgc aggtgccggt tctgactggc
    17221 agccgaaaaa ctcaccaccg cagtatgccg gtccaattcg cttacgtcag gggctgggtc
    17281 agtcgaaaaa cgtggtgatg gtacgcgcaa tgcgggcgat gggcgtcgac tacgctgcag
    17341 aatatctgca acgcttcggc ttcccggcgc aaaacattgt ccacaccgaa tcgctggcgc
    17401 tgggttcagc gtccttcacc ccaatgcagg tggcgcgcgg ctacgcggtc atggcgaacg
    17461 gcggcttcct ggtggacccg tggtttatca gcaaaattga aaacgatcag ggcggcgtga
    17521 ttttcgaagc gaaaccgaaa gtagcctgcc cggaatgcga tattccggtg atttacggtg
    17581 atacgcagaa atcgaacgtg ctggaaaata acgatgttga agatgtcgct atctcccgcg
    17641 agcagcagaa tgtttctgta ccaatgccgc agctggagca ggcaaatcag gcgttagtgg
    17701 cgaagactgg cgcgcaggag tacgcaccgc acgtcatcaa cactccgctg gcattcctga
    17761 ttaagagtgc tttgaacacc aatatctttg gtgagccagg ctggcagggt accggctggc
    17821 gtgcaggtcg tgatttgcag cgtcgcgata tcggcgggaa aaccggtacc actaacagtt
    17881 cgaaagatgc gtggttctcg ggttacggac cgggcgttgt gacctcggtc tggattggct
    17941 ttgatgatca ccgtcgtaat ctcggtcata caacggcttc cggagcgatt aaagatcaga
    18001 tctcaggtta cgaaggcggt gccaagagtg cccagcctgc atgggacgct tatatgaaag
    18061 ccgttcttga aggtgtgccg gagcagccgc tgacgccgcc gccgggtatt gtgacggtga
    18121 atatcgatcg cagcaccggg cagttagcta atggtggcaa cagccgcgaa gagtatttca
    18181 tcgaaggtac gcagccgaca caacaggcgg tgcacgaggt gggaacgacc attatcgata
    18241 atggcgaggc acaggaattg ttctgattaa aaaggcgctt cggcgccttt tcagtttgct
    18301 gacaaagtgc acttgtttat gccggatacg ggtgaacgcg ttatccggct aacaaaatcg
    18361 tgaaaactca atatattgca ggaaccccat aagcctgata agcattgtgc atcaggcaaa
    18421 cttcacgcat ttacactcgc ccctgccctt tcaaccattc gcgcacgagg aacagcgcgc
    18481 tgacattacg cgcttcgttg aagtcagggt cttccagcaa atccatcata tgcgccagcg
    18541 gccagcgcac ctgtggtagc ggctctggct catcgccttc cagcgattcc gggtagagat
    18601 cttgcgctac cacgatattc attttgctgg aaaagtaaga cggtgccatg ctgagcttct
    18661 tcaaaaaagt cagatcgttc gcaccaaatc caacctcttc ttttagctcg cggttagcgg
    18721 cttcgaagac gctttcaccc ggatcaatta atcctttcga aaaacctaat tcgtaggatt
    18781 cagttcccac tgcatattcg cggatcagga tcaggtgatc gtccacaatc ggcacaatca
    18841 tcactgcttc ccggttggtt ggacgcattc gttcataaac acgccgcacg ccattgctga
    18901 actctagatc cacgctctcg acggtaaaca gtcgggaacg ggctacagtt tcaacattca
    18961 gaatggtggg tttttgtaat gatttgctca tcgtgggatc tatgctgtga aatcagcggt
    19021 tattgtgcga tatcggacac gctttcggca atgtgaattg catgttattt acatttatgt
    19081 aacttaataa ataatcgtcc tcaaatcaaa ttaaaagtca ataggttgaa ataactccag
    19141 gaatttgctg atattccgcc ttcagagggt ttgctatgat cagcggttac tgtgatgtgc
    19201 ttaatgatgc tcaagttaaa ctccacgctt gccgatagcc aaccgcagaa tcatgtattg
    19261 tgtccggtgc gactgaccac gcctgacaga ctaagtaaga tggggaaagc atgagcacca
    19321 ttgtgatttt tttagctgct ttgctggcct gctcactact tgcgggatgg ctgataaaag
    19381 tgcgatccag acggcgtcag ctgccctgga ccaacgcctt cgcggatgcg caaacgcgta
    19441 aactcacacc tgaagaacgt agcgccgttg aaaattatct tgagagcctg acgcaggtat
    19501 tacaggtgcc tggcccaacg ggagccagcg cggcaccgat ctctctggcg ctgaatgccg
    19561 aaagcaacaa cgtcatgatg ctgacacacg ctatcacgcg ttacggcatc tctaccgacg
    19621 atccgaataa atggcgttac tacctcgatt cggtagaagt ccacctgccc cctttctggg
    19681 agcagtacat caacgatgag aataccgttg aactgattca taccgattcg ctgccgctgg
    19741 ttatttcact caacggtcat acgctgcagg agtacatgca ggaaactcgc ggctatgcct
    19801 tgcaacctgt tccgtccacg caggcgtcga ttcgcgggga agagagtgag caaatcgagc
    19861 tactcaatat tcgcaaagaa acgcatgaag aatatgcgct gagtcgtccg cgcgggctgc
    19921 gtgaagcgtt gctgatcgtc gcctccttcc tgatgttctt tttctgcctg attaccccgg
    19981 atgtatttgt tccgtggctg gcaggcggcg cgttactgct gctgggcgca ggtctgtggg
    20041 ggctattcgc ccccccggca aaatcctccc tgcgggaaat tcattgtctg cgcggtacac
    20101 cccgtcgttg gggattgttt ggcgaaaacg atcaggaaca gatcaacaat atttcactcg
    20161 gtattatcga cctggtctat cccgcacact ggcagccata cattgctcag gatctcggtc
    20221 aacaaaccga tattgatatc tatctcgacc gccacctagt gcgtcagggg cgatatcttt
    20281 cgctgcatga tgaagtaaaa aactttccgt tacagcactg gctgcgcagt acgattatcg
    20341 ctgcgggctc gctgctggtg ctgtttatgc tgttattctg gatcccgctg gatatgccgc
    20401 tgaaattcac tctctcatgg atgaaaggtg cgcagaccat tgaagccacc agcgtaaaac
    20461 aactggctga cgctggcgtg cgggtaggcg atacattgcg tattagcggt acgggaatgt
    20521 gtaatattcg cacttccgga acctggagcg cgaaaaccaa ttcacctttt ttaccgtttg
    20581 actgctcgca gatcatctgg aacgacgccc gctcattacc attaccagaa tctgaactgg
    20641 tcaacaaagc gacggcattg actgaagcag ttaatcgcca gctgcaccct aaaccggaag
    20701 atgaatctcg cgtcagcgcc tcattacgtt cagcaattca aaaatccggc atggtattgc
    20761 ttgatgattt tggcgacatt gtactgaaga cagcggatct gtgttctgcc aaagatgact
    20821 gtgtgcgact gaaaaatgcg ctggtcaatc tcggcaacag taaagactgg gacgcgctgg
    20881 taaaacgcgc caacgccggg aagctcgatg gcgtgaatgt gttattacgc ccggtgagtg
    20941 cggaatcgct ggataacctg gtggcaacct ccaccgcgcc gtttatcacg catgaaacgg
    21001 cgcgagcggc acaatcacta aacagtccgg cccccggcgg attcctgatt gtcagcgacg
    21061 aaggcagcga ttttgttgat cagccctggc cttcggcatc actttacgac tacccgccgc
    21121 aagaacagtg gaacgctttc cagaaactgg cacaaatgct gatgcatacg ccgtttaacg
    21181 ccgagggtat cgtcacaaaa atcttcactg acgccaatgg tacgcagcat ataggccttc
    21241 atccgatccc ggatcgttcc ggcctgtggc gctatctcag caccacgctg ctgctactga
    21301 cgatgctggg tagcgccatt tataatggcg tacaggcctg gcggcgttac cagcgtcatc
    21361 gcactcgcat gatgaagatt caggcctatt atgaaagctg cctgaacccg caactgatca
    21421 ccccttcaga aagccttatc gaataacacg tttgcgcggc aggttatgct accctgtcgc
    21481 gcaaattgct tcactctgga gattttcctc atgcatatca acattgcctg gcaggacgta
    21541 gataccgttc tgctggatat ggacggcacg ttgctcgacc tcgccttcga taactatttc
    21601 tggcaaaagc tggtgcctga aacatggggc gcgaaaaacg gggttacgcc acaggaagcg
    21661 atggaatata tgcgccagca atatcacgac gtacagcata cgctaaactg gtactgtctt
    21721 gattactgga gtgagcaact gggtctggat atctgtgcga tgaccaccga gatgggaccg
    21781 cgtgccgtac tgcgtgaaga taccattccg tttcttgagg cactgaaagc cagcggtaag
    21841 cagcgaattt tgctcaccaa tgcgcatccg cacaacctgg cggtaaaact tgagcatacc
    21901 ggtctggacg cacaccttga tttattactt tccacccaca catttggtta tccgaaagag
    21961 gatcagcggt tatggcatgc ggtggccgaa gctacgggtc tgaaagctga aagaacgctg
    22021 tttattgatg acagcgaagc gattctcgat gctgccgcgc aatttggtat tcgttactgc
    22081 ctcggcgtga ctaatcctga ctcagggatt gccgagaaac agtatcaacg ccatccgtca
    22141 ctgaatgact accgccgcct gatcccctcg ctaatgtgaa ggagacgcca tgaaagaaaa
    22201 acctgctgtt gaggttcgac tggataaatg gctatgggct gcccgttttt ataaaacccg
    22261 cgcgctggcc cgtgaaatga ttgaaggcgg taaggtgcat tacaacgggc agcgcagcaa
    22321 gccgagcaaa atcgtcgagc tgaatgccac gctcactctg cgccagggaa atgacgaacg
    22381 cacggtgatt gtaaaggcga ttactgaaca gcgtcgcccc gccagcgagg cagccttgct
    22441 gtatgaagag actgcggaaa gtgtagagaa acgcgaaaaa atggcgctgg cacgtaaact
    22501 taatgcctta accatgccgc acccggaccg acgcccggac aaaaaagagc gccgcgacct
    22561 gttacgattt aaacacggcg acagtgaata actgtcacct gcaagagaga tgattatgcc
    22621 gcaacatgac caattacatc gctatctgtt tgaaaacttt gccgtgcgcg gcgaactggt
    22681 aaccgtttcg gaaaccctgc aacagatcct tgagaatcac gattatccgc agcccgttaa
    22741 aaacgtgctg gcagaactgc tggttgcgac cagcctgtta accgctacgc tgaagtttga
    22801 tggtgatatc accgtacagc tgcaaggcga cggtccgatg aatctggcgg ttattaacgg
    22861 taacaacaac cagcagatgc gcggtgtggc gcgcgtgcag ggcgaaattc cagaaaatgc
    22921 cgacctgaaa acgctggtcg gcaatggtta cgtggtgatc accattaccc cgagcgaagg
    22981 tgaacgctat cagggcgtgg tgggtctgga aggtgatacc ctggcggcct gcctggaaga
    23041 ttactttatg cgttctgaac agctgccgac gcgcctgttt attcgcaccg gcgacgtaga
    23101 cggcaaaccg gctgcaggcg gtatgttgtt gcaggtaatg cctgcgcaaa atgcccagca
    23161 ggacgacttt gaccacctgg cgacgctaac cgaaaccatc aaaaccgaag aactgctgac
    23221 cttaccggca aacgaagtgt tgtggcgttt gtatcacgaa gaagaggtga cggtttacga
    23281 tccgcaggat gtggagttca aatgcacctg ctcgcgtgaa cgttgcgccg atgcgctgaa
    23341 aacgctgcct gatgaagaag tcgatagcat cctggcggaa gatggcgaaa ttgacatgca
    23401 ttgtgattac tgcggtaacc actatctgtt caatgcgatg gatattgctg aaatccgcaa
    23461 caacgcgtct ccggcagatc cgcaagttca ttaatgagta tgtccggcag agagagctct
    23521 gccggatttt acgactaata cacctgatgc gctttgtcac tcattcagat tcacatccag
    23581 cctttcacca tccactacaa gatcgcgcca cgctttaggc ttcgttccca gttttccatc
    23641 cttcgcagct gtgagtaatt tttctttcgt tatatcacgc ggaagtaaac tcatcgtaag
    23701 ctcttgccat tctttcttgt caggatcaaa gccatagaca atgtatcttt catcatcaaa
    23761 ggcaaagagg atccgctctg ccctgccatc actattcaga tcttgctcga ccagtacgca
    23821 ggcgtctttt tcaatacagg tcatcacgtt atagcgatcc tggattaagg ccgaccagaa
    23881 tgtcgcatcc ggtttaccag aaccaggggc aattaacaca ttttcggcta atactttttc
    23941 cgatatctgt tgctgaagat gttgctctcc atctaacgcc atcagtagat cccgcgcgcg
    24001 tttcgggtct ttcatatact cggcatcgct tttcagcgac tcaagcgccg cacgtccata
    24061 gcgaccgctg tgttcgagca tgtagatagt tacctggtct ggcgtgtttt tgccgctctg
    24121 ataacgcgcc atatgactgt tcacactaat gcgcatactg tccagcaccg gcgaattaag
    24181 aagcaccagt attaccaaca ccaataacga aaccgcaagg ttcactttgc cctgaagaac
    24241 aaggggattt tgccctttac gccagacgat actgacaaaa taccccagcg accataccag
    24301 taacaccagc accgccagcg cgccctgcaa gcggtcgaca gtccagccgt actgagcgac
    24361 ccgcagccat aacgcccagg cggcgatgaa cacatacagc ggagcaacca gcagagcggt
    24421 tttaatcagg caacgcaacg gccccgtcca gggaagtgac gctttttgcg gatcgcggac
    24481 aatagccatt aagatcaatt gcaaaaaggc cagcgttaac agcaacccgg cggcggagat
    24541 gtggcgagaa atcgcactca ggcctgtaaa cggcagggtg atgataaaca tcagggttag
    24601 caatgatact aacggcagca accccgtggc gattaacgtg aacaactttt gaatagagtc
    24661 gattaaacgt gactgtgttc gcgcgaggat caccgccagc gcggtaacca gacctaacgt
    24721 gagataaata aaccagtcgg ttgcaaaaaa gagcgttttg aaaaacgtga tgccaacaag
    24781 tttaaacaac tcactccaca gtaaaagcac cagccacgtc agaccattgg cgagaaaaat
    24841 caccagtaat ataagtacgt tatgccatac tgactggtaa aaatagcgat agcggctact
    24901 gtcattgcgg atgcgaaggc tttgttgtat ccacggtagc aacaacattg ccatcagcag
    24961 cagatagcaa ccaaaatccc aaagcgcctt ttcagctctc cagggggtca tgccatcagt
    25021 ttgccacttc agccagccgc tcatccccag cgtggcaata aacaccagcg ccagccatcc
    25081 ccaaaggcgc ttctgtttaa aagagatcac ggagaagagt aaaacggatg aaaacgcgac
    25141 ggtcgcaggc acgccataaa caatccaact gtgatttttt ccggaaagcc aggcgatcag
    25201 caggtagcaa accagaccct gaagtaatcc ggtagcaatc atcccccagc gtgttgcggg
    25261 tgaaagttca acgttatcca tgttggttat ccagaatcaa aaggtgggtt aattatcgca
    25321 tccgggcagt agtattttgc ttttttcaga aaataatcaa aaaaagttag cgtggtgaat
    25381 cgatacttta ccggttgaat ttgcatcaat ttcattcagg aatgcgattc cactcacaat
    25441 attcccgcca tataaaccaa gatttaacct tttgagaaca ttttccacac ctaaaatgct
    25501 atttctgcga taatagcaac cgtttcgtga caggaatcac ggagtttttt gtcaaatatg
    25561 aatttctcca gatacgtaaa tctatgagcc ttgtcgcggt taacaccccc aaaaagactt
    25621 tactattcag gcaatacata ttggctaagg agcagtgaaa tgcgcgttaa caatggtttg
    25681 accccgcaag aactcgaggc ttatggtatc agtgacgtac atgatatcgt ttacaaccca
    25741 agctacgacc tgctgtatca ggaagagctc gatccgagcc tgacaggtta tgagcgcggg
    25801 gtgttaacta atctgggtgc cgttgccgtc gataccggga tcttcaccgg tcgttcacca
    25861 aaagataagt atatcgtccg tgacgatacc actcgcgata ctttctggtg ggcagacaaa
    25921 ggcaaaggta agaacgataa caaacctctc tctccggaaa cctggcaaca tctgaaaggc
    25981 ctggtgacca aacagctttc cggcaaacgt ctgttcgttg tcgacgcctt ctgtggtgcg
    26041 aacccggata ctcgtctttc cgtccgtttc atcaccgaag tggcctggca ggcgcatttt
    26101 gtcaaaaaca tgtttattcg cccgagcgat gaagagttgg caggtttcaa accagacttt
    26161 atcgttatga acggcgcgaa gtgcactaac ccgcagtgga aagaacaggg tctgaattcc
    26221 gaaaacttcg tggcttttaa cctgaccgag cgcatgcagc tgattggcgg cacctggtat
    26281 ggcggcgaaa tgaagaaagg gatgttctcg atgatgaact acctgctgcc gctgaaaggt
    26341 atcgcttcta tgcactgctc cgccaacgtt agcgagaaag gcgatgttgc ggtgttcttc
    26401 ggcctttccg gcaccggtaa aaccaccctt tccaccgacc cgaaacgtcg cctgattggc
    26461 gatgacgaac acggctggga cgatgacggc gtgtttaact tcgaaggcgg ctgctacgca
    26521 aaaactatca agctgtcgaa agaagcggaa cctgaaatct acaacgctat ccgtcgcgat
    26581 gcgctgctgg aaaacgtcac cgtgcgtgaa gatggcacta tcgactttga tgatggttca
    26641 aaaaccgaga acacccgcgt ttcttatccg atctatcaca tcgataacat cgttaagccg
    26701 gtttcaaaag cgggtcacgc aacgaaagtt atcttcctga ccgcagacgc ctttggtgtg
    26761 ctgccaccgg tttctcgtct gactgccgat caaacccagt accacttcct ctctggcttc
    26821 accgccaaac tggccggtac tgagcgtggc atcaccgaac cgacgccaac cttctccgct
    26881 tgcttcggcg cggcattcct gtcgctgcac ccgactcagt acgcagaagt gctggtgaaa
    26941 cgtatgcagg cggcgggcgc gcaggcttat ctggttaaca ctggctggaa cggcactggc
    27001 aaacgtatct cgattaaaga tacccgcgcc attatcgacg ccatcctcaa cggttcgctg
    27061 gataatgcag aaaccttcac tctgccgatg tttaacctgg cgatcccaac cgaactgccg
    27121 ggcgtagaca cgaagattct cgatccgcgt aacacctacg cttccccgga acagtggcag
    27181 gaaaaagccg aaaccctggc gaaactgttt atcgacaact ttgataaata caccgacacc
    27241 cctgcgggtg ccgcgctggt agcggctggt ccgaaactgt aatgatttga agctggagaa
    27301 tatctatcca gtatcttata gaaagcaaaa cgggaggcac cttcgcctcc cgtttattta
    27361 cccttctttt gtcgtgctct gcgcccgcgt taccggcaca ggcagccagg cgcgaatgga
    27421 aagcccgccc cgctcgctgg tgccaagctc cagcatcccg ttatggttat ccacgatacg
    27481 ctgcacaatc gccagcccta atcccgtgcc gctaatggtg cgtgcactgt cgccgcgaac
    27541 aaacggctgg aaaaggtgct tacgttgttc cggcgcaatt cccggaccgt catcttccac
    27601 ctggaaccag gcgcgattcg gctccgtccc gctgctgact ttgatccagc cattgccgta
    27661 acgggcggcg ttgaccacca tattcgccac cgcgcgtttg atcgacagcg ggtgcatttt
    27721 cacttcaatg ctgccggggt aaagcgcggt ttcaatttcc cgctcatagc cactttcggc
    27781 agcaataacc tcaccgagta ctgcgttgag atccgccatt tccatcggca tctcctgtcc
    27841 ggtgcgcagg tagtcgataa actgctcaat gatggcattg cactcctcga tatctttatt
    27901 gatcgattct gccaggtagc catcctgctc gctcatcatc tcggtcgcca ggcgaatacg
    27961 cgtcagcggt gtacgcaagt cgtgacttac ccccgccatc agcagcgtgc ggtcatccgc
    28021 cagttgctta acaccagccg ccatatggtt aaaggcgcgg gtaacggaac gcacctcgga
    28081 cgcgccatac tcacgcagcg gcggcggaat aatcccttta ccaacctgca aggctgcgtg
    28141 ttcgagatcg accaacggtc gattctggat acgaataaac agccacgccc cgcctatcgc
    28201 caatagcata atcgccaacg tatagcggaa cagcggagag aaatcgccct gatgaatttc
    28261 ggtcagcggc acgcgtaccc agatattggg cgacagccag gttttcagcc agacgacagg
    28321 cgaacttttg ttgacctcaa cgcgcacttc cgtcgggccg cccagttgct gtgccatctg
    28381 atggcttaag aattcatagt gttgcgccca acgcagacct gcctcttcgg cagcctcgtt
    28441 ggagtagaga gagatcccca gctcacggta gatctcccga cggaaagcgg gaggcacaac
    28501 caactgcgtg ccgtcctcca gttgcagttt gtcggtcatc aacatacgca cttcgtacgc
    28561 gaggacttta ttaaactgct ggaggctcgg caaaatcgcg aagttcagca ccaccagata
    28621 agtcgtcacc aggctggcga acagcaaggt gacgatgagc aataacgtac gggcaaatga
    28681 acttcgtggc gagaagcgca atcgcctcat gctttagagc cgtccggtac aaagacgtag
    28741 cccaggcccc aaacggtctg aatgtaacgc ggatgcgctg gatcttcttc caccatgcgg
    28801 cgcagacgag aaatctgcac gtcgatggag cgttccattg cggaatattc acgaccacgg
    28861 gcaaggttca tcagcttatc gcgggagagc ggctcacgcg gatggctgac cagtgccttc
    28921 agtaccgcaa actcaccgct ggtgagcggc atcggctcgt cttcgcggaa catttcgcgc
    28981 gtaccgaggt taagtttgaa cttaccgaaa gcaattaccg cctcttcctg tgacggtgcg
    29041 cctggtagct cgttcgcctg acgacgcagc accgcacgga tacgggccag cagttcacgc
    29101 gggttaaacg gttttggaat gtaatcgtca gcgccaatct ccaggccaac gatacggtcc
    29161 acttcttccc ctttcgccgt caccataatg atcggcatcg ggttgctctg actacgaaga
    29221 cgtcggcaaa tcgacaagcc atcttcacca ggtaacatta aatccagtac cataagatgg
    29281 aaagattcac gagtcagcag gcgatccatc tgttctgcat tagcgacgct tcgaacctgg
    29341 aagccttgtt cggtgagata acgttccagc agcgcacgca ggcgcatgtc gtcatcgacc
    29401 accagaatct tgtagttctc ttgcattgtt tgtactccca aaggttcgca acaatttgta
    29461 agcgtgtatt cttaaaaaag ctcacgttcg tcaccagcta aatctggtat gaatttcagc
    29521 ctaaattgtt acaaagcata ttaaacagca gcttaagtat acaatttatt cggcgaaaca
    29581 ttattgattc tgttgatatg atcacgttat acccaatgtg cgcattatca aacagacaaa
    29641 gggaatcaac gagatgaaaa cgcccctggt tacccgggaa gggtatgaaa aactcaaaca
    29701 agagcttaat tatctctggc gtgaagaacg cccggaggtc acaaaaaagg tgacctgggc
    29761 cgcaagtctg ggcgaccgca gcgaaaatgc tgactatcag tataataaaa agcgcctgcg
    29821 tgaaatcgac cgtcgcgtgc gctatctcac taaatgcatg gaaaatctca aaatcgtcga
    29881 ttactccccg cagcaggaag gcaaagtctt ttttggcgcg tgggtggaga ttgaaaacga
    29941 cgatggcgtg actcaccgtt tccgtattgt cggctacgat gaaatttttg gccgtaaaga
    30001 ttacatctct atcgattccc cgatggcccg cgcattgctg aaaaaagaag tcggcgatct
    30061 ggctgtggtg aatactcctg ccggggaagc gagctggtat gttaatgcta tcgagtacgt
    30121 gaaaccgtaa ggaagagtct taacctcctg ccgatggctg gcatttttgc cagccagtcc
    30181 gtataactat cccctgattt ttgatccgaa aagatgaact caaaccatga tgaatgattc
    30241 gttctgccgc attattgcgg gtgaaattca ggcgcgcccg gaacaggttg acgctgccgt
    30301 tcgcctgctt gacgaaggga ataccgtgcc ttttattgca cgttatcgta aggaaatcac
    30361 cggcggtctg gatgacacgc agctgcgtaa tctggaaacg cgtctgggat acctgcgcga
    30421 gctggaagag agacgtcagg cgatcctcaa gtccatttcc gagcaaggca aactcaccga
    30481 tgatctggcg aatgccatca acgccaccct aagcaaaacc gaactcgaag acctctacct
    30541 gccctacaaa cctaaacgcc gcacccgcgg gcaaatcgcc attgaagcag ggcttgagcc
    30601 gttggctgac ctgctgtgga gcgatccgtc acacacgcca gaagtcgccg ctgcgcaata
    30661 tattgatgcc gataaaggcg tggcagatac caaagccgcg ctggacggcg cgcgctatat
    30721 cctgatggaa cggtttgccg aagatgccgc gctgctggcg aaagtgcgtg attatctgtg
    30781 gaagaacgcg catttggttt ctacggtggt gagcggtaaa gaagaggaag gggcgaaatt
    30841 ccgcgactat ttcgatcatc acgaaccgtt gtccacggtg ccttctcacc gcgcgctggc
    30901 gatgttccgt gggcgtaacg aaggcgtact tcagctttcg ctgaatgccg atccacagtt
    30961 cgatgagccg cccaaagaga gctattgcga gcaaatcatc atggatcacc ttggcctgcg
    31021 cctgaacaat gccccggcgg atagctggcg caaaggcgtg gtgagctgga cgtggcgcat
    31081 caaggtgctg atgcatctgg aaaccgaact gatgggcacc gtgcgcgaac gtgcggaaaa
    31141 tgaagcaatc aacgtctttg cccgtaacct gcacgatctg ctgatggctg cccctgccgg
    31201 actgcgtgca acgatgggcc tcgatccggg tctgcgtacc ggggtaaaag tggcggtggt
    31261 cgatgccact ggcaaactgg tagcgaccga taccatttac ccgcacaccg gacaagccgc
    31321 aaaagcagcg atgactgttg ctgcgctgtg tgaaaagcat aacgttgaac tggtggcgat
    31381 cggcaacggt acagcttccc gcgaaaccga acgtttctat ctcgacgtgc agaagcagtt
    31441 cccgaaagtg accgcacaga aagtgatcgt cagcgaagct ggcgcgtcgg tttactcggc
    31501 ttccgagctg gcagcgcagg agttcccgga tctcgacgtt tcgctgcgtg gcgcggtttc
    31561 tatcgcccgc cgtttgcagg atccgctggc ggagctggtg aaaatcgatc cgaaatctat
    31621 cggcgtaggt cagtatcagc atgacgtcag ccagacgcaa ctggcccgca aactggatgc
    31681 agtagtagaa gactgcgtaa acgccgttgg cgtcgatctc aacaccgctt ctgttccgct
    31741 attaactcgc gtggcgggcc tgacgcgaat gatggcgcaa aacatcgtgg cctggcgcga
    31801 tgagaacggc cagttccaga accgtcagca actgctgaaa gtcagccgtc tggggccgaa
    31861 agccttcgag cagtgcgcgg gcttcctgcg cattaaccac ggtgacaacc cgctggacgc
    31921 ctctaccgtt cacccggaag cctatccggt ggtggaacgc attctggcag caacacagca
    31981 ggcactgaaa gatctgatgg gtaacagcag cgaactgcgt aacctgaaag cgtctgactt
    32041 tactgatgaa aaattcggtg tgccgacagt aactgacatc atcaaagagc tggaaaaacc
    32101 gggtcgcgat ccgcgtccgg aatttaaaac cgctcagttt gccgatggcg tcgagacaat
    32161 gaacgacctg caaccgggta tgatcctcga aggcgctgtc accaacgtca ccaactttgg
    32221 cgcgtttgtc gatatcggcg tgcatcagga cggcctggtt cacatctctt cattgtcgaa
    32281 caagtttgtg gaagatccgc ataccgtggt gaaagcgggc gacattgtga aggtgaaagt
    32341 gctggaagtg gatcttcagc gtaaacgtat cgccctgact atgcgtctgg acgagcagcc
    32401 tggcgaaacc aactctcgtc gcggcggcgg taacgatcgc ccgcaaaaca accgcccggc
    32461 agccaaacca cgcggtcgtg aagcgcagcc tgccggtaac agcgcgatga tggacgcgct
    32521 ggcggcagca atgggcaaaa aacgttaaac gcccggactg gcccctaaac ctgcgcgata
    32581 aggcgttcac gccgcatctg gcaacgaacg ccttgcctga cataaaagtg ccggagaata
    32641 tctccggcat atttattcca cagccaaact cacaatatat ttcgttaata tttatcactt
    32701 cattaacaac tgaaacctta attaaacatt agccagtcct ggtaattcgc tattcgaatt
    32761 atattttcgc tgcgatataa ccttgagcca catcaacatt gagtcagatt attattcaaa
    32821 ccaacattcg cacacatttt aagtattgct gatagaaacc attctcatta tcattgtact
    32881 attgattatt taatctctcc ttcgttggca aatcatctgg tctcatgtcg ctgtcaaacg
    32941 ccccatgagg tagttatcca gttaatgaga aacaagtagg cacctatgca atacactcca
    33001 gatactgcgt ggaaaatcac tggcttttcc cgtgaaatca gcccggcata tcgccaaaaa
    33061 ctgctttctc ttggcatgtt acctggctcc tcttttaatg tggtgcgcgt cgctccactc
    33121 ggcgacccca ttcatatcga aacccgtcgt gtgagcctgg tattacgcaa aaaagatctg
    33181 gccttattag aagtggaagc ggtttcctgt taatacggtg ataacaacaa tgaaaaaatt
    33241 aaccataggc ttaattggta atccaaattc tggcaagaca accttattta accagctcac
    33301 tggcgcacgt cagcgtgtag gtaactgggc tggcgttacc gtcgaacgta aagaagggca
    33361 attctccacc accgatcatc aggtcacgct ggtggacctg cccggcacct attctctgac
    33421 caccatctca tcgcagacct cgctcgatga gcaaatcgcc tgtcactaca ttttgagtgg
    33481 cgacgccgac ctgctgatta acgtggtgga tgcgtctaac cttgagcgta acctgtacct
    33541 gacgctacaa ctgctggaac tcggcattcc ctgcatcgtg gcactgaaca tgctcgacat
    33601 tgccgagaag caaaatattc gtattgaaat tgatgctctg tcggcgcgtc ttggctgccc
    33661 agtgatcccg ctggtttcaa cccgtggtcg cggtattgaa gcgctcaagc tggcgattga
    33721 tcgctataaa gctaacgaga atgtggaact ggtgcattac gcacagccgc tactcaacga
    33781 agcagattca ctggcaaaag tgatgccttc cgacatcccg ctgaaacaac gtcgctggct
    33841 gggcctgcaa atgctggaag gcgatatcta cagccgcgcc tacgccggtg aagcgtcgca
    33901 gcatcttgat gccgccctcg cccgtctgcg taatgagatg gacgatccgg cgctgcacat
    33961 tgccgatgcg cgttaccagt gcattgctgc catctgtgat gtggtaagca acaccctgac
    34021 ggcagaaccc agccgtttca ccactgcggt agataaaatc gtgctcaacc gtttcctcgg
    34081 tctgccgatt ttcctctttg tgatgtacct gatgttcctg ctggctatca acatcggcgg
    34141 ggcgttacag ccgctgtttg acgtcggctc cgtggctcta tttgtgcatg gtattcaatg
    34201 gattggctac acgctccact tcccggactg gctgactatc ttcctcgccc agggcctggg
    34261 tggcggtatt aacaccgtgc tgccactggt gccgcagatc ggcatgatgt acctgttcct
    34321 ctccttcctt gaggactccg gctatatggc gcgtgcggcg tttgtgatgg accgcctgat
    34381 gcaggcgctg ggcttgccgg ggaaatcctt tgtgccgctg atcgtcggtt tcggttgtaa
    34441 cgtaccgtcg gtaatgggtg cacgtacgct tgatgcaccg cgtgaacgtc tgatgaccat
    34501 catgatggca ccgtttatgt cctgcggcgc gcgtctggct atcttcgcag tattcgcggc
    34561 tgccttcttc gggcagaacg gtgcgctggc ggtcttctcg ctgtatatgc tcggtatcgt
    34621 gatggcggtg ctgactggcc tgatgctcaa gtacaccatc atgcgcggtg aagcgacgcc
    34681 gtttgtcatg gagctgccgg tctaccatgt accacacgtt aaaagcctga ttatccagac
    34741 ctggcagcgt ctgaaaggct tcgttctgcg tgctggtaaa gtgattatca tcgtcagcat
    34801 tttcctgagc gctttcaaca gcttctcgtt gagcgggaaa atcgtcgata acatcaacga
    34861 ctcggcgctg gcttccgtca gccgggtgat caccccggtc ttcaagccga ttggcgtgca
    34921 tgaagataac tggcaggcaa cagttggcct gtttacgggt gcaatggcga aagaagtggt
    34981 ggtgggtacg ctcaacaccc tctacaccgc agaaaatatt caggacgaag agttcaatcc
    35041 ggcggaattt aacctcggtg aagagttgtt cagcgcggta gatgaaacct ggcagagcct
    35101 gaaagacacc ttcagcctta gcgtactgat gaaccccatt gaagctagca aaggcgacgg
    35161 cgaaatgggt accggggcga tgggcgtgat ggatcagaaa ttcggtagcg cagctgccgc
    35221 atacagctac ctgattttcg tcctgctgta tgtaccatgt atctcggtga tgggagccat
    35281 cgcccgtgaa tcaagccgtg gctggatggg cttctccatc ctgtgggggc tgaatatcgc
    35341 ttactcactg gcaacattgt tctatcaagt cgccagctac agtcagcacc caacttacag
    35401 cctggtgtgc attctggcgg ttatcctgtt taacatcgtg gttatcggtc tgctgcgccg
    35461 cgcgcgtagc cgggtggata tcgaactgct ggcaacccgc aagtcggtaa gtagttgctg
    35521 cgcagccagc accaccggtg attgccatta atggcttcac ttattcaggt gcgtgatttg
    35581 ctggcgttac ggggccgtat ggaagcggcc cagataagcc agacattgaa cactccacag
    35641 ccaatgatta acgccatgct gcaacaactg gaaagtatgg gcaaagccgt gcggattcag
    35701 gaagaacctg acggctgcct ctccggcagt tgtaaaagct gcccggaagg aaaagcctgt
    35761 ctgcgcgagt ggtgggcgct gcgttaacca tactccatcg cctgttttga aaagcccggt
    35821 atgcgtttgc atccgggctt ttttgcgtgc ggctttccat aaaaatgcaa ttcttgcagc
    35881 gcgacgtaag ttcctctgaa agcgtctcgc agggatgaaa actggctaat gcacaggtgt
    35941 ggagtggcgc gtagagtcgc ggcattcaaa caacaggtga aggaacgcca tgagcaaaaa
    36001 gcagagttcc accccacacg atgcgctgtt caaactcttt ttacgccaac cggagacggc
    36061 acgcgatttt cttgcgtttc atttaccagc acccattcac gcgctctgtg atatgaaaac
    36121 cctcaagctg gagtcgagca gctttattga tgacgatctg cgtgaaagct attccgatgt
    36181 gctgtggtcg gtgaaaacag aacaaggacc aggatacatc tattgtctga ttgaacatca
    36241 aagcacctca aacaaactga tcgcatttcg catgatgcgt tacgctattg ccgcaatgca
    36301 aaatcacctt gatgccggat acaaaacgtt gccgatggtg gtgccattgt tgttttacca
    36361 cggtattgaa agcccctatc cctattcgct gtgttggctg gattgtttcg ccgatcccaa
    36421 actggcaagg cagctttatg cctccgcatt tccgctgatt gatatcaccg tcatgcctga
    36481 tgatgaaatc atgcagcacc gacgcatgac gctgctggag ttaattcaaa aacatattcg
    36541 tcaacgcgac ctgatggggc tggtagagca aatggcctgc ttattaagta gtggatacgc
    36601 taatgacaga caaatcaaag ggctgtttaa ttacatactg caaaccggcg atgcggtacg
    36661 ttttaacgat tttatcgacg gcgttgccga acgatcaccg aaacacaagg agagtttaat
    36721 gactattgcg gaaagattgc ggcaggaggg ggaacaatcc aaagccctgc atatagccaa
    36781 aataatgctt gaatccggag tccctcttgc agacatcatg cgctttaccg ggctgtcaga
    36841 agaagagttg gctgcggcga gtcagtaaag ttctgtctcg ccattcaaaa gtgcctacac
    36901 cctctgcttc aacgccacca gcacgtgacg aaactcgacc ggatgcgaaa taaacggcgc
    36961 atgagccgct ttggcgaaga tgtatgactc gctgtgtggc cagagtttat ccagcatcgg
    37021 caccactttg cgcggcacca gaccatcgag atagccatac agtcgcaaaa acggcatggg
    37081 tacgttttgc agcggcaagc ggagatcaac cgttttgagg atttccagcc caccattgag
    37141 cacgtcaacc tccggcatcg gtaacgccag aacggttttc ttcaacgccc gcgcatcctg
    37201 acgcgccgtt tcagtaccca tggtttgtaa cgcaaggaac cgttccaccg tacgctgaaa
    37261 atcatcactg agttgctgct gaaatcccgc cagcacgtcc ggttttatcc ccggccactc
    37321 gtcacgagca ctaaaacaag gtgacgacgc cacggtgacc agcgcctgaa cacgctcggg
    37381 atgggttaac gcaatctggc ttgccaccag cccgcccaga ctccagccta accaaatggc
    37441 tttatcaggt gcctgtcgca gcacggcttc ggccatctcc gcaagtgaca gcgcaccaaa
    37501 tccccggcta cgcccgaagc cgggcaggtc aacaagatgc agcgtaaaat gcgagctaag
    37561 ttcctcgtca atgcaacgcc acacttcagc attcagtccc catccgtgca gcagcacaag
    37621 atgaacattc ccctgacctt tggtctgcca ccagatgtta ttcatccgct attgttctct
    37681 tttgacttac aaggatgaac atatgctaac agtaccggga ttatgctggc tatgccgaat
    37741 gccactggca ttaggtcatt gggggatttg ttcggtctgc tcacgcgccg cccgtacaga
    37801 taaaacgtta tgcccacaat gtggattacc cgccacacac tcccatcttc cctgcggtcg
    37861 ctgcctgcaa aaaccgccgc cctggcaaag actggtcacg gttgccgact atgcgccgcc
    37921 gttaagtctg ctcatccacc agcttaagtt ttcccggcgc agcgaaatcg ccagcgccct
    37981 gtcacgtctg ttactattgg aagtcttaca cgctcgtcgc accaccgggt tgcaattgcc
    38041 ggatcgtatc atcagcgttc cgttatggca gcggcgtcac tggcgtcggg gatttaatca
    38101 gagcgatttg ctgtgtcagc cgttatcacg ctggttgcac tgccaatggg atagcgaagc
    38161 catcacacgt acacgggcca ctgcgaccca gcattttctt agcgcccggc tacgcaggcg
    38221 caacctgaaa aatgcctttc gccttgaatt gcctgtgcaa ggtcgccata tggtgattgt
    38281 ggatgatgtc gttaccaccg gaagtaccgt cgcagagatt gcgcagttgc ttttacgcaa
    38341 tggtgcggcg actgtccagg tctggtgcct ttgtcgaacc ttgtagagcc tcgatgatgg
    38401 gcgtattata accaactaaa atagtcaact attaggccat tactatgatc cgtatttccg
    38461 atgctgcaca agcgcacttt gccaaactgc tggcaaatca ggaagaaggg acacaaatcc
    38521 gcgtatttgt gattaaccct ggcacgccta acgctgaatg tggtgtttct tattgtccgc
    38581 cggatgccgt ggaagccacc gacaccgccc tgaaatttga cctgctgacc gcgtatgttg
    38641 atgagttaag cgcaccatac ctggaagatg cagagatcga ttttgttacc gaccagttgg
    38701 gttctcagct aacactgaaa gcgcctaacg ccaaaatgcg taaagtggca gacgatgcac
    38761 cactgatgga gcgcgtggag tatatgctgc agtcgcagat caacccacag cttgctggtc
    38821 acggtggccg cgtttcactg atggaaatca ccgaagacgg ttacgccatt ctgcaatttg
    38881 gcggcggctg taacggttgt tccatggtcg atgtgacgct gaaagaaggg atcgagaagc
    38941 agctactaaa cgaattcccg gagctgaaag gtgtgcgcga tctcaccgaa caccagcgcg
    39001 gcgaacactc ctactactaa gttatcccct cttttgtaga ttgcccgatg cgacgctaaa
    39061 gcgtcatttc gggcctacgt tgaacatgtg ccagacgttg gcgcagcatt gctttggcaa
    39121 atccctccct tcgtttcccc gcgataatat gaccaacctc tcataattta aatttacccc
    39181 gctctggtga ttctcaaacg ccagatgtta cccgtatcat tcacgtgggt accaaacata
    39241 ctcctgacat ctgactacaa taattagttt tagtgggtat cagtcgtggt gccgcaatat
    39301 ctctgttccc gattgggata attagagctt gtcgtcagaa aattgacgtt acccataaca
    39361 aatgaaaggc caggtaaatc atgccattag tcattgttgc tatcggtgta atcttgttat
    39421 tactcctgat gatccgcttc aaaatgaacg gcttcatcgc tctcgtcctc gtggcgcttg
    39481 cggttggatt aatgcaaggg atgccgctgg ataaagttat tggctccatc aaagccggtg
    39541 tcggcgggac gctcggtagc cttgccctga tcatgggttt tggcgcaatg ctgggcaaaa
    39601 tgctggcaga ctgcggtggc gcacaacgta tcgccaccac gctgattgcc aaatttggta
    39661 aaaaacacat ccagtgggcg gtggtactga ccggttttac cgttggtttt gccctgttct
    39721 atgaagtggg ctttgtgctg atgctgccgc tggtgtttac catcgcggct tctgcgaata
    39781 ttccactgct gtatgttggt gtaccaatgg cagctgcact gtctgtgacc cacggcttcc
    39841 tgccaccgca tccgggtccg actgcgattg ccaccatttt caatgccgat atgggtaaaa
    39901 ccctgctgta cggtactatt ctggcaatcc cgaccgtgat tctcgccggt ccggtttacg
    39961 ctcgcgtgtt gaaaggtatc gataagccaa ttccggaagg tctctacagc gcgaaaacct
    40021 tcagcgaaga agagatgccg agctttggcg tcagcgtctg gacctctctg gtgccggtag
    40081 tactgatggc gatgcgtgcg attgccgaaa tgatcctgcc gaaaggtcac gctttcctgc
    40141 cggtagcgga gttcctcggt gacccggtaa tggctacgct gattgccgtg ctgattgcga
    40201 tgttcacctt tggtctgaac cgtggtcgtt caatggatca gattaacgac acgctggttt
    40261 cttccatcaa aatcattgcg atgatgctgt tgatcatcgg tggtggcggt gcgttcaagc
    40321 aggtgctggt agacagcggc gtggacaaat acattgcttc catgatgcac gaaaccaaca
    40381 tttctccgct gctgatggcc tggtcgattg ctgccgtact gcgtatcgcg ctgggttccg
    40441 caaccgttgc ggcaatcact gcgggtggta tcgcggcacc gctgattgca acgacgggtg
    40501 ttagcccgga gctgatggtt attgcggtgg gttccggtag tgtgattttc tctcatgtga
    40561 acgatccggg cttctggctg ttcaaagagt actttaacct gactatcggc gagaccatca
    40621 aatcctggtc gatgctggaa accattatct cggtgtgcgg tctggtaggc tgtctgctgc
    40681 tgaatatggt gatttgataa cactgactgc cggatgcatc gcatccggca tcaaccgcac
    40741 tctacttctt cttcgctgca gctctgcgcc gtctgtccaa atccttcagc aacttgttca
    40801 cgccatcatc ggcaaacatc gtctcaagcg ttgcggaaag cttgcgtcgc cagtttttat
    40861 actggtaact ggtgccagga atattcaccg gttcggccat atccagccag tcttccggct
    40921 gtagtcctaa cagagcactg ttactgtcgg caatgtagcg ctgcaaacca cgattaagcg
    40981 tcggcgtcat cgacatcaac gatgccttat gcccggcacg ttttggcaga caaccatatt
    41041 tatgcagtgc atccagcagc ccttgcttcg ccagttcgcg atcctgatac agaccgcgca
    41101 gtaccacttc atccggatac agccccaggg ttttgcccag cgttagatcc ccgctctccc
    41161 agtagccgcg tagcgtaggc aggtcatgtg tcgccgcaac cgccatcgac tgctccggat
    41221 acgctttcgg tgcacggaac gtcttctcgt ggtcgttttc gaaatagagc actttgtagg
    41281 agtacacacc gctgctgcgc agcttaccga caatctctac cggtacggta ccgagatctt
    41341 caccaatcac catacagcga tgacgtttac tttcgagtgc cagaatcgag agcagatcat
    41401 ccaccggata gtgaacatac gcgccctgat ctgccgtctc gccatacggt atccaccaca
    41461 aacgcagcat cgacatcaca tggtcaattc gtaatgcgcc gcagttttgc atattggcac
    41521 gcaacagctc gataaacggt tcataagcac gcgcggtgat gatatgcggg tccattggcg
    41581 gcaatcccca gttctgcccc aacgggccga ggatatccgg cggcgcgcca accgacgctt
    41641 tcaggcaata cagttcacgg tcgcaccagg tttccgcccc accttccgct acgccaaccg
    41701 ccagatcacg atacaagcca atcggcattt catagccctg gcttatctcc cagcaggcgg
    41761 caaactggct gtaagcctgc cactgcaacc agagataaaa atcaacgtca tcgcgatgtt
    41821 cttcgcagaa ctgacgcact tctggtgaat ccacgttctg atacatctct ggccatgcag
    41881 gccagcccca gcgcatttcg tcctctttca cttgctgggc atgtagcgca tcaaaggctg
    41941 cctgccagaa caggctgtcg ccttgatctg caacaaactg gcgaaacgcg gccatctgct
    42001 catcatcacg ttgcgcgaaa cctttccacg ccattcgtaa tgctgtcatt tttagggcgg
    42061 taaccgtgga gtaatcgacc cagtcggcat cccgcgcctg ttgcagcgtc tgttgcgtgg
    42121 tcggcaactg ccaccaggcc tgagcctctt cgctaagatg gaaatcttcg acggcgttaa
    42181 cgtcgatata aatcacattc agccagcggc gagaagacgg gctatacggg ctggcgctct
    42241 ccggatttgc cggatagagc gcatgaatcg ggttcagacc aatgaacgat ccgccacgtt
    42301 ttgccacatc cactagcatc gctttgagat cgccaaaatc cccaataccc cagttttttt
    42361 ccgatcgcag cgtataaagc tggacgcagg caccccacag cttttgttta ttcagcaacg
    42421 cctgcggttc gtaacagcgt ttcggggcga caatcaccct gcaatgcgcg cgctggtctt
    42481 cctgggtaag cgtcagcgtg tgataacctt ccggcagctt cgtcggtaga ttgaacgctt
    42541 tgccccccgt tatatggcct ttgtactgcg ttccttcttc ggtggtcagc agccagctat
    42601 attcgccgct gccctccacc accatcggca tttttttgcc gctggtataa accatgacat
    42661 tcgggactgg cgttaccgcc actttcgtgg cggtacgttg atgcatcgcg tcaagcaaac
    42721 gccgtttggt ttcggcgcta atcgactgcg gtttaccgtg ggcattgatg taatgagggc
    42781 taatccccgc cgccagcgcg gcattatcca gacgtttgct ttccatcgag cttccttagc
    42841 gttttgcctg ccagatacga gcctgataat cgcgaataga gcgatccgag ctaaacatac
    42901 cgcagcgggc ggtattgagg atcgccgcgc gagtccaggc ctcctggtcg cggtacagca
    42961 catccacctg cttttgtgcc tctacatagg ctgcgaaatc cgccatcacc agatacggat
    43021 cgccgccctg tttgccgata ctgtgcagca tctggtcgaa ggcatgctta tcgccgtcgc
    43081 tgtatttacc gctttccagc tctttcaata ctgcgtccag caccttatct ttcttccgcc
    43141 atttcaccgg gtcgtagcct ttggccagaa ttgccttcac ttgttccacg gtatgaccaa
    43201 aaataaagat attttcttca ccgactttct cggcgatttc aacgttcgcc ccatccagcg
    43261 taccgacagt aagcgcacca ttgagcgcca gtttcatatt gccggtaccg gaagcttctt
    43321 tacctgcagt cgaaatttgt tcggagatat ccgccgccgg gatcagtttt tccgccgccg
    43381 aaacgcaata atccggcagg aacaccacct tcaacttatc gccaaccagc ggatcgttgt
    43441 tgatcacgtc agccactttg ttgatcgcaa agataatatt tttcgccagg tagtagcccg
    43501 gtgccgcttt cgcgccgaag aggaagacgc gcggtacgcg atcagcctgt gggttttcac
    43561 gaatttcttt gtacaacgcc agaatatgca gcagattcag gtgctggcgt ttgtactcat
    43621 gcagacgttt gatctgaata tcgaaaatcg cctgtggatt gatctcaata ccggtacgaa
    43681 ctttcacaaa ctccgccaga cggactttat tcgcctgctt gatctcgcga tattgctgac
    43741 ggaatttcgc atcatcagcg aatttttcca gattgatcag ctgatcgaga tcgttagccc
    43801 actctttttt cagtgattta tccaacagag ccgccagtgc cgggttgcac tgtttgatcc
    43861 agcgacgtgg ggtaataccg ttggtgacgt tatggaattt gttcggccat agctggtgat
    43921 attccgggaa cagatctttc accaccagat ccgagtgcag cgccgcaaca ccgttcaccg
    43981 cgaaaccgcc aaccacacac aggttcgcca tatgcacttg tttgtcgtgc accaccgcca
    44041 gtttagccca cactttttca tcgcccggcc aggttttctc caccagcgtt ttaaagcgag
    44101 tattaatttc gttaataatc tgcatgtggc gcggcagtaa gcctttcacc agtttcacat
    44161 cccagcgttc cagcgcttct ggcatcaggg tatggttggt gtaagcgaaa gttttgctgg
    44221 taatggccca ggcgtcatcc cagctcatct ggtgctcatc gatcagcacg cgcaacagtt
    44281 ctggaatcgc gatagttggg tgggtatcgt tcagctgaat aacttcgtaa tccgccagtt
    44341 cgtgcagttt acgccccgcc agatgatggc gacgcaaaat atccgctacc gaacaggcac
    44401 actggaagta ctgctgcatc aagcgcagct ttttaccggc agtatggttg tcgtttggat
    44461 agagaacttt ggtcagtttt tccgcattga tgccctgctg ttcggcacgc aggaaatcac
    44521 cgtcgttaaa tttagtcaga tcaaacggat gcgcgtgcgt cgcctgccac agacgcagcg
    44581 gctgcgccac gccattacga tagccgacaa cggggagatc ccacgcttga ccggtaatgg
    44641 taaactccgg ctcccagcgt ccgtctttcg tcactttacc gccaatccct acctgcacat
    44701 ccagtgcttc gttgtggcgg aaccacgggt agttactgcg atgccagtca tccggcgctt
    44761 caacctgttt gccatccaca aaagactggc ggaacaaacc atattgatag ttcagaccgt
    44821 aacccgtcgc agactgaccg acagttgcca ttgaatcgag gaagcacgcc gccagacgtc
    44881 ccagaccacc gttacccagc gcagggtcga tctcttcttc cagcaggtca gtcagattga
    44941 tgtcataagc cttcaacgaa tcctgtacat cctgatacca gccgagattc aacaggttgt
    45001 tgcccgtcag acgaccaatc aaaaactcca ttgagatgta gttaacatgt cgctgattcg
    45061 ccaccggctt ggcgaatggc tgagcacgca gcatttcggc cagtgcttca ctcactgcca
    45121 gccaccactg gcgaggagtc atttcagccg cagaatttaa gccataacgc tgccactgac
    45181 gtgaaagcgc ttcctgaaat tgcttatcgt taaaaatagg ttgtgacata ggagttccac
    45241 ttttcttaga ttttcaacac aacgttatcg ctagtttgcc aggctcgatg ttgaccttcc
    45301 tcatcctgcg ggggattagg cagggaggag ttgcggggat gagcaaggaa atgtgatctc
    45361 gaccacttaa agctagtgca aaccacagga ttagcagcaa atcaatgcca tacagcgcag
    45421 aaaatctgta tctaagtgca aaaaatggcc gttgcgtatt ttcaaaaagc gaaaggtaac
    45481 tctataaatt aagtaaagga gtgaaacagt ttcacaagta aaatatccag tgtgctccat
    45541 ctcattctta atagatttat taagatcatc tttttagata gcactttcat caggaatgaa
    45601 gaagaaaccc ttgcttaaat gaatctgatg aacataaggg aaaccagtat tcacgctgga
    45661 tcagcgtcgt tttaggtgag ttgttaataa agatttggaa ttgtgacaca gtgcaaattc
    45721 agacacataa aaaaacgtca tcgcttgcat tagaaaggtt tctggccgac cttataacca
    45781 ttaattacga agcgcaaaaa aaataatatt tcctcatttt ccacagtgaa gtgattaact
    45841 atgctgattc cgtcaaaatt aagtcgtccg gttcgactcg accataccgt ggttcgtgag
    45901 cgcctgctgg ctaaactttc cggcgcgaac aacttccggc tggcgctgat cacaagtcct
    45961 gcgggctacg gtaagaccac gctcatttcc cagtgggcgg caggcaaaaa cgatatcggc
    46021 tggtactcgc tggacgaagg tgataaccag caagagcgtt tcgccagcta tctcattgcc
    46081 gccgtgcaac aggcaaccaa cggtcactgt gcgatatgtg agacgatggc gcaaaaacgg
    46141 caatatgcca gcctgacgtc actcttcgcc cagcttttca ttgagctggc ggaatggcat
    46201 agcccacttt atctggtcat cgatgactat catctgatca ctaatccagt gatccacgag
    46261 tcaatgcgct tctttattcg ccatcaacca gaaaatctca ccctggtggt gttgtcacgt
    46321 aaccttccgc aactgggcat tgccaatctg cgtgttcgtg atcaactgct ggaaattggc
    46381 agtcagcaac tggcatttac ccatcaggaa gcgaagcagt tttttgattg ccgtctgtca
    46441 tcgccgattg aagccgcaga aagcagtcgg atttgcgatg acgtttctgg ttgggcgacg
    46501 gcactacagc taatcgccct ctccgcccgg cagaataccc actcagccca taagtcggca
    46561 cgccgcctgg cgggaatcaa tgccagccat ctttcggatt atctggtcga tgaggttttg
    46621 gataacgtcg atctcgcaac gcgccatttt ctgttgaaaa gcgccatttt gcgctcaatg
    46681 aacgatgcac tcatcacccg tgtgaccggc gaagaaaacg ggcaaatgcg cctcgaagag
    46741 attgagcgtc aggggctgtt tttacagcgg atggatgata ccggcgagtg gttctgctat
    46801 cacccgctgt ttggtaactt cctgcgccag cgctgccagt gggaactggc ggcggagctg
    46861 ccggaaatcc accgtgccgc cgcagaaagc tggatggccc agggattccc cagcgaagcg
    46921 attcatcatg cgctggcggc aggcgatgcg ctgatgctgc gcgatattct gcttaatcac
    46981 gcctggagtt tgttcaacca tagcgaactg tcgctgctgg aagagtcgct taaggccctg
    47041 ccgtgggaca gtttgctgga aaatccgcag ttggtgttat tgcaggcgtg gctgatgcaa
    47101 agccaacatc gctacggcga agttaacacc ctgctggccc gtgctgaaca tgaaatcaag
    47161 gacatcagag aaggcaccat gcacgcagaa tttaacgctc tgcgcgccca ggtggcgatt
    47221 aacgatggta atccggatga agcggaacgg ctggcaaaac tggcactgga agagctaccg
    47281 ccgggctggt tctatagccg cattgtggca acctcggtgc tgggtgaagt gctgcactgc
    47341 aaaggcgaat tgacccgctc actggcgcta atgcagcaaa ccgaacagat ggcacgccag
    47401 cacgacgtct ggcactacgc cttatggagt ttaattcagc aaagtgaaat tctgtttgcc
    47461 caagggttcc tgcaaaccgc gtgggaaacg caggaaaaag cgttccagct gatcaacgag
    47521 cagcatctgg aacagttgcc gatgcatgag tttctggtgc gcatccgagc acaactgtta
    47581 tgggcctggg cacggctgga tgaagccgaa gcgtcggcgc gtagcgggat tgaagtctta
    47641 tcgtcttatc agccacagca acagcttcag tgcctggcaa tgttgattca atgctcgctg
    47701 gcccgtggtg atttagataa cgcccgtagc cagctgaacc gtctggaaaa cctgctgggg
    47761 aatggcaaat atcacagcga ctggatctct aacgccaaca aagtccgggt gatttactgg
    47821 caaatgaccg gcgataaagc cgccgctgcc aactggttgc gtcatacggc taaaccggag
    47881 tttgcgaaca accacttcct gcaaggtcaa tggcgcaaca ttgcccgcgc acaaatcttg
    47941 ctgggcgagt ttgaacctgc ggaaattgtc ctcgaagaac tcaatgaaaa tgcccgcagt
    48001 ctgcggttga tgagcgatct caaccgtaac ctgttgctgc taaatcaact gtactggcag
    48061 gccggacgta aaagtgacgc ccagcgcgtg ttgctggacg cattaaaact ggcgaatcgc
    48121 accggattta tcagtcattt tgtcatcgaa ggcgaagcga tggcgcaaca actgcgccag
    48181 ctgattcaac tcaatacgct gccggaactg gaacagcatc gcgcacagcg tattctacga
    48241 gaaatcaatc aacatcatcg gcataaattc gcccatttcg atgagaattt cgttgaacgt
    48301 ctgctaaatc atccggaagt gccagaactg atccgcacca gcccgttgac ccaacgtgaa
    48361 tggcaggtcc tggggctgat ttactccggt tacagcaacg aacaaatagc gggtgaactg
    48421 gaagtggcgg caaccaccat caagacgcat atccgcaatc tgtaccaaaa actcggcgtc
    48481 gcccaccgtc aggctgccgt gcagcacgcg cagaaactgc tgaagatgat gggttatggg
    48541 gtgtgagttt gtatagcgtt tttatgtaca ctctttatac tgtatataaa atcgcactac
    48601 aggctcatga atatgaaacc ggaaagcaaa gaagcgccaa tcaatatcag agcgaaagcc
    48661 tcacagcgtg acctgattga tatggcagcg aatctggtcg caaaatcgcg cactgatttt
    48721 atgctggatg ccgcatgccg tgaagcgcaa gacatcttat tagatcaacg actattcatc
    48781 cttgatgacg agcaatacga tgcgtttctc gctgccctgg acgcccccat cactgctgag
    48841 cgtcaggcca aaattaacgc gttgatgaat cgtaaatcgc catgggaata acggctccta
    48901 ctcccctaac gtctgaacat aatctggctg acttttgctg ttccgatcac gggatgaatg
    48961 aatggctaaa aaagaaagcg ttaaaaaatc acagctccgg tctgtctcgt gtttacgtta
    49021 tctgtattgc caatacccgc caggtaatcg gctattactg cctttctaca ggcagtattc
    49081 aacgtaattt ggcccctgga gccatgcgac gtaatgctcc cgaatcgttg cctgttgtcg
    49141 tattaggcag actggcaatt gatcaagcct gggcaggtaa agggttgggc gtcgcattgt
    49201 tgaaagatgc agtctatcgc acaatgtcca tagctcaaca ggtaggggtt cgtgcgctca
    49261 ttgttcatgc cttagacgat tcagtacgca atttctatct gaagtatgcc tttgtacctt
    49321 caccattcca gtcacttacc ttactgtatc ccattactct ggagtaattt acctctaccg
    49381 gatagtcaca ctgatgagtc tgatccggga gggatttcaa tcagtgagtt tggttatcat
    49441 cacccgcgtt acgccatctg tttcagccag agtaaaccgc accggcaaga aacgctccac
    49501 cacggcgata ttggtcagca gatggcatga gggatgggcg accgtaaatt gtcccgcgcc
    49561 cgccagcgcc attggcaaca ctagctggtc agcaagatat tcccccactg ccgccgggct
    49621 tgccaggtag cgtttcacct ctttcaccaa ctgtgccgca accacctcgg cactgacgcg
    49681 cttttcaccg acgacaaaaa agcgttcggt gatattttca ctttcgactt caagcgagac
    49741 ggtattaccc ggcccctggt cgcgcggcag gttatgaata ttctgctcat gcagggaaaa
    49801 acttgccgcc agcgtagcga tttcacgctc agcgacatgc cgcggcacgc cagctaataa
    49861 cacctctcca cgcatctgca caatgttccc gcgctcgcca agttgcaagg tgttaaacga
    49921 tgccaccggc gagacttccg ttgccaccac gccgcctccg gcaggataaa aaccgtgacg
    49981 taacagcgtg gtttgctgat gaattcctat tttcgccagc agcggctcca acacccggcg
    50041 gataaaatcc gcaggcgggg ccgacgggtt atcggtaccg ccgctcactt caacacgcga
    50101 aggtccatcg gcaaaccaca gcgcgggcag caccgtttgc agcaccagcg tacaacttcc
    50161 tgcgctaccg atagcaaagc gataatcgcc gccacgcacg gtgccgggcc ggaagaccag
    50221 acgctgcgac cccagctccg cgccttccac cgtcgccccg caaatttccg tcgccgcctt
    50281 taccgccgtc agatgctggc gcaacagtcc cggttttgcc cgcccggcac gaatgccggt
    50341 gatggtaaat ggctggccgg ttatcatcga cagactcagc gccgagcgca tgatctgccc
    50401 gccgccttcg ccctgtgcgc catccagcgc aatcatcctt ttcatcattt atccttttac
    50461 gcacaccacc tgacgtaggg tgtagataac ttccaccaga tcgctttgtg ccgccatcac
    50521 cgcatcaata tctttatacg ccatcgggat ttcgtcgatc acttcggcat ctttacggca
    50581 ttccacatgc acggtggcac gaatttgatc ttccacgctg aacatttttt tcgctttagt
    50641 acggctcata acccgcccgg caccgtggct gcacgaacag aacgactctt catttcccag
    50701 cccacggacg ataaagcttt tcgctcccat cgaccctgga ataattccat attgaccagc
    50761 acgcgcagac accgcgcctt tacgcgtcac gtagatctct tcaccaaagt gctgttcttt
    50821 ttgcacatag ttgtggtgac agttgatctc ttccatcgcc agcgtttgtg gctgtctgac
    50881 cgttttctgc gtaatgctct gcaacgccgt tactacgttt tccatcatca catcgcggtt
    50941 aaggctggca aaaagctgcg cccaggccac ggctttcagg taatcatcaa agtattccgt
    51001 accttccata aagtacgcca gatcacgcga cggcaatgtt tcaagcgttt cctgcatctc
    51061 tttttgcgcc agatcgataa agtaagtccc gatggcatta ccaattccgc gtgaaccgga
    51121 gtgcagcata atccacacct ggtccgactc atcaaggcag atttcaataa agtggttacc
    51181 cgttcccagc gttcccaggt gtttatagtt gttagtgtta aggaaacgcg gatatttttg
    51241 cgttaaccac tgataaccgg cctccagctc agcccatttc gcatcgacgt taacaggcgg
    51301 gttttcccat gcgcctttat cacgtttaca acggccagtg gtacgcccgt gcggcaccgc
    51361 agtttcaatc gcctgacgca gctccgccag gttttccggc aggtcagccg ccgttaaagc
    51421 ggtacgtagt gcgttcattc cacagccaat atccacgccc accgccgccg gaataatcgc
    51481 ccctttggtc gggatcacgc taccaatggt ggaaccttta cccaggtgta catcaggcat
    51541 taccgcaata tgtttgaaaa taaacggcat cttcgccgta ttaataagtt gctgacgcgc
    51601 atcggcttct accggcacgc ctttggtcca catttttacc ggggcatttt cagtggtcag
    51661 taattcgtaa ttcattttgt ttttctcttt tcgttgtttg cggtcctgat aattgcaacc
    51721 gtcgtgccag aaaattaaat aagcagatta attttttaat tcattgtttt aaaaagatta
    51781 tatctttacg tccgtaaacg gagatttccc gcaaagccaa tttaccgata atgaaatatc
    51841 gtcttttata aggatatcta agatgcgtaa aacagtggct tttggctttg tcggaacagt
    51901 gctggattat gccgggcgcg gcagtcagcg ctggtcgaaa tggcgtccgt cactctgtat
    51961 atgccagcaa gaatcgttag tcatcgatcg actggaatta ttgcatgacg cccgctcgcg
    52021 ctcgctattt gaaacgctta aacgcgatat cgccagcgtt tcgccagaaa ccgaagtggt
    52081 gagcgttgag attgaactgc ataacccgtg ggatttcgaa gaggtttacg cctgcctgca
    52141 tgatttcgcc cgtggttacg agtttcagcc agaaaaagaa gactatttaa ttcacatcac
    52201 caccggcacc cacgtcgcgc agatttgctg gtttctgctg gctgaagcgc gttacctgcc
    52261 cgcccggttg atacaatctt cacctccgcg caaaaaagaa cagccgcgcg gcgcaggtga
    52321 agtgacgatt atcgatctcg atttaagcct ttataacgcc atcgccagcc gctttgccga
    52381 ggaacgccag caaacgcttg attttcttaa gtccggtatt gccacgcgta acccccactt
    52441 caaccgcatg attgagcaga ttgaaaaagt agcgattaaa tcccgcgcac cgatcctgct
    52501 aaacggccca accggtgcgg gcaagtcatt tctggcgcga cgtatatttg aattaaaaca
    52561 ggcgcggcat cagtttagcg gcgcgtttgt ggaagtgaac tgcgcaaccc tgcgcggcga
    52621 taccgccatg tcgacgctgt ttggtcatgt gaaaggcgcg tttaccgggg cacgggaatc
    52681 tcgtgaagga ttattacgca gcgccaacgg cggaatgttg tttcttgatg agattggcga
    52741 actgggcgca gacgaacagg caatgctgct gaaagccatt gaagaaaaaa ccttttatcc
    52801 gtttggcagc gatcgccagg tgagcagtga ttttcagctt atcgccggaa cggtgcgtga
    52861 tttgcgccag ctggttgccg atggcaaatt ccgcgaagat ctatacgcgc ggatcaatct
    52921 ctggaccttc accctgccgg ggctacgcca gcgtcaggaa gatattgaac ctaacctgga
    52981 ttatgaagtg gagcgccacg cttcactcac cggcgacagc gtgcgtttta acaccgaagc
    53041 gcggcgcgcc tggctggcct ttgcgacatc tccccaggca acatggcgcg gtaactttcg
    53101 cgagctttct gccagcgtca cgcgaatggc aacctttgcc tctagcggac gcattactct
    53161 ggacgtggtt gaagatgaga taaaccgtct gcgctataac tggcaggaga gccgcccctc
    53221 cgcgcttacg gcgttgctgg gcgcagaggc agaaaacatc gatctcttcg accgtatgca
    53281 actggaacac gttatcgcaa tctgccgcca ggcaaagtcg ctttccgcag ccggacgcca
    53341 gctttttgac gtttcgcgcc agggcaaagc cagcgtcaat gacgcggatc ggctacgcaa
    53401 atacctggcg cgttttggtc tgacgtggga agccgtgcag gatcagcaca gctccagttg
    53461 aatatggtgg tccgtcagca cctgcatcac gcttactggc ggcggggtgt cggtgtagac
    53521 ggcatctacc atgctgatgc tacccatatt gaccatcgcg ttacggccaa atttcgagtg
    53581 atcgacaacc agcataacgt ggcgcgagtt ctcaataatg gcgcgtttgg tgcgaacttc
    53641 gtggtaatcg aactccagca gcgagccgtc gctatcgatg ccgcttatcc ccagaatgcc
    53701 gaaatcaagg cggaactggg agataaaatc gagcgtcgct tcgccaatga tcccgccatc
    53761 gcggctgcgt aattcgccac cggcgagaat aatacggaaa tcctctttta ccatcagcgt
    53821 gttagcaacg ttaagattgt tggtgacaat gcgcaaattg ctgtgattga gcagcgcgtg
    53881 cgccaccgct tccggcgtgg tgccgatatc gataaacagc gtcgagccat tggggatttg
    53941 ctccgccact ttgcgggcga tgcgctcttt ttcttcggtc tgggtggctt tacgatcgtg
    54001 ccacggcgtg ttaaccgaac tggaaggcag cgccgcaccg ccatgatggc gcaggatcag
    54061 gttttgctcc gccagctcat tgaggtcgcg gcgaatagtc tgcgggctga cggagaagtg
    54121 ctccaccagc tcctcggtac tgacataacc ctgctgttta accagttcga taataccgtt
    54181 gtgacgttgt gtttgtttca tttctaaatc cctggaatta ttttcgtttt cgcgcattga
    54241 gcgaatcaac aaaagccatc gctaaaccca cggctaaccc ggcgatgtgt gctccgttcg
    54301 ccatcgacat cccaaacaaa tcaaaccatc cggcgacaat ccagatcagc gcaaagataa
    54361 ttaacccacg ttgcaggtaa atgccacttt gcggatcgcg ttcgccacgt agccagacgt
    54421 agcccatcag cgcatacacc acgccagaaa gcccgccaaa ccacggcccg ctgaattttt
    54481 gctgcacata gccgcttaac agggcgctga taagcgtaat gacaattagc ttaccgctac
    54541 cgaggcgttt ttccaccgca ccgccgagat accaccacca gagcaggtta aagaggatat
    54601 gcatcagcga gaagtgcatt aacgcgtggg tgaagtaacg ccagaactca aatttcagtg
    54661 ctggatcgaa tggccaagcc agccataaca tcacttcctg atcgccgaga atttgcatgg
    54721 caataaacac caccacgcag gcgatcatca tcacccaggt caccggacct gcgcgttcac
    54781 gcaaggcggc aaagaaagga taacggcgat aatgcaggcc actgccggta tggcctgcct
    54841 gccagctcgc cgccagataa cgcggatctg ccgggttttc gagaaaacgc gccagctccg
    54901 cccgtacgcg ctcggcctgg gactcatccg ccagccagac atcgctttgg ttatgttgtt
    54961 gaatcgtgag gataacaccc tgcgtcgcca tgtaatcaac aaacgcctgc gccacgcggg
    55021 ggttagcaaa agaggtaatc atcaacatcg ttgctgtcgc ttattccaca caaaagggga
    55081 cagtataaag cgttacgcgc cgtacgccac ctctgcggga aactgacgtt gccaggcttc
    55141 aaagccgccg tcaatgctat agaccacatc gtagccctgt tgcagcagat actgcgccgc
    55201 gcctttgctg ctattgccgt gataacacat caccatcacc ggagtgtcaa agtcgttatt
    55261 acgcataaaa gcgcccagcg tgtcgttggt taaatggaaa gcctgcaccg catgtcccat
    55321 tgcgaaactc tgtggatcgc gaatatcgac cagcaccgcc tctttttcct gcaacttctg
    55381 gtgcgcgtcg gcaacgttaa tacattcgaa ctgatccatg cgtctctctt tctttacaaa
    55441 caagtgggca aatttaccgc acagtttacg tcgaagcggc agataaacgc cataatgtta
    55501 tacatatcac tctaaaatgt tttttcaatg ttacctaaag cgcgattctt tgctaatatg
    55561 ttcgataacg aacatttatg aactttaacg aaagtgaatg agggcagcat ggaaaccaaa
    55621 gatctgattg tgataggtgg cggcatcaat ggtgctggta tcgcggcaga cgccgctgga
    55681 cgcggtttat ccgtgctgat gctggaggcg caggatctcg cttgcgcgac ctcttccgcc
    55741 agttcaaaac tcattcacgg tggcctgcgc taccttgagc actatgaatt ccgcctggtc
    55801 agcgaggcgc tggctgaacg tgaagtgctg ctgaaaatgg ccccgcatat cgccttcccg
    55861 atgcgttttc gcctgccaca tcgtccgcat ctgcgcccgg cgtggatgat tcgcattggt
    55921 ctgtttatgt acgatcatct gggtaaacgc accagcctgc cgggatcaac tggtttgcgt
    55981 tttggcgcaa attcagtgtt aaaaccggaa attaagcgcg gattcgaata ttccgactgt
    56041 tgggtagacg atgcccgtct ggtgctcgcc aacgcccaga tggtggtgcg taaaggcggc
    56101 gaagtgctta ctcgtactcg tgctacttct gctcgtcgcg aaaacggcct gtggattgtg
    56161 gaagcggaag atatcgatac cggcaaaaaa tatagctggc aagcgcgcgg cctggttaac
    56221 gccaccggcc cgtgggtgaa acagttcttc gacgacggga tgcatctgcc ttcgccttat
    56281 ggcattcgcc tgatcaaagg cagccatatt gtggtgccgc gcgtgcatac ccaaaagcag
    56341 gcctacattc tgcaaaacga agataaacgt attgtgttcg tgatcccgtg gatggacgag
    56401 ttttccatca tcggcaccac cgatgtggaa tacaaaggcg atccgaaagc agtgaagatt
    56461 gaagagagtg aaatcaatta cctgctgaaa gtgtataacg cgcactttaa aaaacagctt
    56521 agccgtgacg atatcgtctg gacctactcc ggtgtgcgtc cgctgtgtga tgatgagtcc
    56581 gactcgccgc aggctattac ccgtgattac acccttgata ttcatgatga aaatggcaaa
    56641 gcaccgctgc tgtcggtatt cggcggtaag ctgaccacct accgaaaact ggcggaacat
    56701 gcgctggaaa aactaacgcc gtattatcag ggtattggcc cggcatggac gaaagagagc
    56761 gtgctaccgg gtggcgccat cgagggcgac cgcgacgatt atgccgctcg cttacgccgc
    56821 cgttatccgt tcctgaccga atcgctggca cgtcattacg cgcgtactta tggcagcaac
    56881 agcgagctgc tgctcggcaa tgcgggagcg gtaagcgatc ttggggaaga tttcggtcat
    56941 gagttctacg aaacggagct gaaatatctg gtcgaccacg agtgggtccg ccgcgccgac
    57001 gatgccctgt ggcgtcgcac aaaacaaggc atgtggctga atgcggatca acaatctcgt
    57061 gtgagtcagt ggctggtgga atatacgcag cagaagttgt cgctggcgtc gtaaattaac
    57121 gtaaggtgat caggtcagat ttcaatctgg cctgagactg atgacaaaca caaaattgcc
    57181 tgatgcgcta cgcttatcag gcctacgtgg tttatgcaat atgttgaatt tgcatgattt
    57241 tgtaggcctg ataaggcgtt cacgccgcat caggcatgaa caaagcgcca cgttgtcaac
    57301 aatctgacct actttttgat tcagggaatt accgcaaagc ccacgccatc atacccaacg
    57361 taacaaaact taagcacgtt ccccactcac tccacttccg tatctggctt ctatttttgc
    57421 tacgccactg gcaataatat cccaacgcaa aaccaagctg tgcacttatc ggaccgacaa
    57481 agtcgatcca ctgttgagac caaaatggcg aaaaattcag tgggagccag acaactgtca
    57541 gaaagaaact gcgcatattt tccagcaaag caatttgcgc tggcgcgaca taaaaaatga
    57601 tattccacag gagcaaccag aaataaactg gtgcaaatac cagccaacaa tgcagtctgc
    57661 ttttcaattc aggtggcgtt ctgcgcgcaa attgccaacc aacgcccacc attgcacaga
    57721 agaaaagcgc atattcagcc gataaccata gttgtaattg cagaagggaa atacgcctgt
    57781 gcatgagcgg gattgagaac agtatttctc ccagaaaact cagcgtgatt acacaggcag
    57841 ccagcgcaac caccgtcggc cacaccaaca acggtacaat aacaaagccg agaaataacg
    57901 gataaatata aagtctgatt atattcatca ttattgtcct gatgattcgg gggatgcttc
    57961 atacagcggc acatacccca catcctctac caactgttgc ccctgctgac ttataaacca
    58021 gtcaacaaat ttctgcgttt ccggcgtggg attttcacgc gtcaccatat aggcatccac
    58081 aatgtatgga taagtgccgt tgcggatatt ttcctcggta ggcgcaacgc catttatagc
    58141 caaaagttta atctgcttgc gcattttgag catattgtca ttcatacgag tgacgtaata
    58201 gtggaaggtg tacccaatgg aagatggcgt gttttgatag tcggcaactt cagtaattaa
    58261 accgcccata agatcaatca cagtggattt tttagctggc agcattgggg tgtcttgcat
    58321 aaccaacccc ttcataatcg tttggcttcc agaatcttca gggcgctgcc agacttttat
    58381 atgttcgtca ctaccaccta ctttattcca gcggctagtt ttgccgctaa aaatatcttt
    58441 aacctggtgc tcagagagag aatttaccgg gttattaata tcaacgataa agacaaaggc
    58501 ttcgcgggcg aaagcggtat atttcagttt aacacccgct tcttctgcac gttttttctg
    58561 tccttccgac ggttgtgcaa caaaaataac cgtcgcggaa cctttaatca gactatcgta
    58621 ggcttcagct gttcgggttt gctgcaaacc atacgcctgc caggccagcg aggttcgatc
    58681 cttatctttt ggctcgggta ctaatttata atacgcggag gcatacagcg ggtataaggc
    58741 tgtagcgccg tacactctcg gccatcttga ccaatcatcg ctgtaacgta atgcaggttg
    58801 cccccgcaga gacgtcagtc catggctctc ttcttccctg acgctgttat agtacccttc
    58861 ccggtcgaca catcccgcca gtaacgccgt gcctaatagt aaaacaccgg tgaatttatt
    58921 catctcgctg attcctttct ctcgagcgtt attccattgt tagatacagc ggcacatatc
    58981 ccacatcttc taccagactc tgcccctgcg gcgttaaaaa ccattcgacc agtttttgtg
    59041 tttctgacgt tgtattttct ctcgtcacca taaatgcatc gacgatgtac gcatatttgc
    59101 cgttgcgaat gttttccgcc gtcggtgtaa taccgttaat cgctagcaat cttatatttt
    59161 tatcagcatt catttgcgtc gcgtaatagc ggaaggtata gcctattgcg ttgtttgtat
    59221 tacggtattc ggcaacgact ttaatcattc cctccatcac gcttgccact tccgtttcct
    59281 gcggcgagat catgcggact tttttcatga cctgtgattg catcactgtc tggctgccag
    59341 agtcttccgg gcgctgccag gtctggatct cctgatcgtt accgccaaca gtacgccaat
    59401 tggtaattgc accactgaaa atgtcacgca cctgttgttc agtcagggaa ttaaccggat
    59461 tatccgcatt gacgatgaaa acaaatgctt cacgggcaaa tggcgtgtat agcaaagtga
    59521 cgcccgattc ctcagcgcgt tttttctgcc cgccggaggg ttgcgccaca aaaataatat
    59581 cggcatcacc tttaacaatc cggttatatg catcgggggt acgagagttc tccagatatt
    59641 cccaaacgtg aaaatcctct ggtattacac ttaatgcata aaatgcagaa gcataaattg
    59701 ggtacgcagc cgtggcgcca tcgattcgcg gccagttttg cctaaactga atttgcggtt
    59761 tgccacgcag cggcgtcagt cgattattga gtttatcggg tcgccaggcc ctgatatcaa
    59821 tattttcgcg tacaccgaca ccaggatttt gctgcttata taaatgggcc tgatacccgg
    59881 cgacaatacc aagtaagaga agtagaaaca ggagtaagtt acggcaacgt agtccggacg
    59941 tgccggataa aggatatctg gtcggccagg cgtatcccag cgaaaaactg atatgcgccc
    60001 caacgggaat aatgaccatc caccagtact caccacccac taatgagact aaaaaaacgc
    60061 ccaaccaggg aagcataagg tttgccaacc aaccagctcc catctggcga aattgataat
    60121 ctgccagatg tagcgtgatt atccagccaa ccagggttaa tagcgcgggt aagagaaaaa
    60181 agcattgccg ccagacggct atttctctgt tttctggcgc gctatcaccg gcccacacgc
    60241 gaccagagaa aaatattatt atcgccccca gcgtcagcat aaagacgttg agtagaagat
    60301 aaattgttgg cgtgataagg atattacaaa cttcaataat accggtaagc ccgacaccca
    60361 gcatggcaac aaccgctgcc aaaaattgcg ccagtatggg gatgccgaaa aaagtcatta
    60421 tcagcgaggt caaaatccat tttctatttg gcatcattct gtccattctt cctgaatgac
    60481 ggaattatac tccccgagaa ccctcgcccc ctctggacac ttttccgaaa taaaggcgaa
    60541 aaaaacggga ccttgtggcc ccgttctgtt ttttagcgaa cttacaatct caccggatcg
    60601 atatgccaga tatgatcagc gtactctttg atagtacggt cagaagagaa gtagcccata
    60661 ttggcaatgt tcagcatcgc ttttgcggtc cactcttcct gaagctcgta gagttcatcg
    60721 actttatcct gacaatcgac atagctgcga taatccgcca gtacctggta gtgatcgccg
    60781 aagttgatca gcgaatcaac cagatcgcga tagcgacccg gatcttccgg actgaataca
    60841 ccgctgccga tttgcgtcag cacctgatgc agctcctcat ctttctcgta gtattcacgc
    60901 ggtttgtagc cctgacgacg cagttcttcc acttcttccg ctgtgttacc aaaaataaag
    60961 atattgtcag caccgacatg atccagcatc tcgacattcg caccgtccaa cgtaccgata
    61021 gtcagcgcac cgttaagcgc aaacttcatg ttactagtgc cggaagcttc cgtccctgcc
    61081 agcgaaatct gttcagacag atctgccgcc ggaatgatca actgcgccag gctaacgctg
    61141 tagttcggga tgaacacgac tttcagctta tcgccaatct gcggatcgtt gttgatcact
    61201 ttcgctacgt cattgatcaa atggataata tgcttcgcca tgtaataggc cgaagccgcc
    61261 ttaccgccaa aaatattcac gcgcggtacc cacttcgcat ccaggtcggc cttgatgcgg
    61321 ttatagcggg taatcacatg caacacattc atcaattgac gtttgtattc gtgaatacgt
    61381 ttgatttgca catcgaacaa cgcctttgga ttcaccacca cattcagctg ctgggcgata
    61441 tactccgcca gacgcttttt attctccagc ttcgcctgat gcacagcgtg attaaccatt
    61501 gggaaatcac agtgttgttg cagctcatta agcaggctaa ggtcggtgcg ccagttacgg
    61561 cccaggtgtt cgtccagcac ggctgaaagc gatgggttcg ctaccgccag ccagcgacgc
    61621 ggcgtcacac cgttggtgac gttggtgaaa cgacccggga agattttcgc aaagtcggca
    61681 aacaacgatt gcaccatcag attagagtgc agttccgata caccgttaac tttgtggctc
    61741 acaacaaccg ccagccaggc catacgcaca cgacgaccgt tggattcatc aatgatcgac
    61801 gcccgtccca gcagatcggt atcgttcgga tactgttcct gcaaggtttt caggaaatag
    61861 tcgttgattt caaagatgat ctgcaggtga cgcggcagaa ttttacccag catatcaacc
    61921 ggccaggttt ccagcgcctc gctcatcagc gtgtggttag tgtaggagaa gacctgacaa
    61981 cacacctcaa acgcgtcgtc ccagctaaat tggtgctcat cgatcagcag acgcatcatc
    62041 tcaggaatcg acagtaccgg atgggtatca ttgagatgaa tcgcgatttt atccgccagg
    62101 ttatcgtagg ttttatgcaa ctgataatgg cggcttaaaa tgtcctgaat ggtcgaggaa
    62161 accaggaagt attcctgacg caggcgcagc tcacgcccgg agtaggtgga gtcatccgga
    62221 tacagtacgc gagatacgtt ctcggagtgg tttttatctt ccactgccgc gaagtagtca
    62281 ccctggttga atttaccgag gttaatttcg ctactggctt gcgcactcca caaacgcagc
    62341 gtgttggtcg cgtcggtgtc gtaaccaggg attatctgat cgtaagcgac tcccagaatc
    62401 tcttcggttt caatccagcg cgttttttta ccttcctgct gaatgcgacc accaaaacgg
    62461 actttatagc gcgtgttgtg gcgtttgaat tcccacgggt taccatattc cagccagtag
    62521 tcaggcgact ctttctggct accgttaacg atgttctgct tgaacatacc gtagtcatag
    62581 cggatgccgt aaccgcgccc cggcaaccct aacgtcgcca gagaatcgag gaagcaagcc
    62641 gccagacgtc ccaggccacc gttaccgagg cccgggtcat tttcttcatc aatcagctct
    62701 tcgagattta accccatcgc ttccagtgcg ccctgtacat cttcgtaaat tcctagcgac
    62761 aacatggcgt tggagagcgt acggccaatc aaaaactcca tcgacaggta gtaaacctga
    62821 cgagtttctt gcgacaactg ggcacggttt gaacgtaacc agcgctccac gagacgatcg
    62881 cgcacagcaa ataacgttgc gttcagccat tcatgtttat tggcgacgac cgggtccttt
    62941 ccaatcgtaa acatcagctt gtaagcgata gagtgcttaa gagcttctac gctaagcgtg
    63001 ggcgatgaat atgtaaacgg agcattcata taggcgtttc ctgaaaacta tttcaagcga
    63061 tagtaaagct cacggtacga cttcgccgcg acctgccagc taaaatccat tgccatagcc
    63121 tgacgttgca caaaccgcca cagagaagga cgggaccaca gtacaaaagc acgtcgaata
    63181 gcccgtaaca gcgaccaggc attactatct tcaaagacaa acccactggc gacgccatct
    63241 gcaaggttct caagagaaca gtcagaaacc gtatcagcaa gcccaccggt gcgccgcact
    63301 aacggcagcg taccgtactt caatccataa agttgcgtta agccgcacgg ttcaaaacgg
    63361 ctgggcacca gaatgacgtc cgcaccgccc ataatgcgat gcgaaaatgc ttcgtgatag
    63421 ccaatctgaa cgcccacctg gccggggtat tccgctgccg ccgcaaggaa accttcctgc
    63481 agcaccggat cgcccgcgcc gagtagcgcc agctgcccgc cctgctccag aagacccggt
    63541 aaggcctcca gcaccaggtc gaggcctttc tggctggtca gacggctcac cactgcaaaa
    63601 agcggcactt tatcgtcaac cttaagcccc attgcgattt gtaactggcg cttattttcc
    63661 gctttatctt ccaacgtatc gcgggtgtaa cgcgaggcca atagtaagtc cgtctctgga
    63721 ctccagattt tctcgtccac gccgttcagt acgccggaaa gacgcccttc acgatgacgc
    63781 tgttgcaaca aaccttccat accgtaggca aactgcggtt cggtgatctc gcgagcgtag
    63841 gttggactga ccgccgtaat gtgatcggca tagtacagac cggccttcag gaaagagatt
    63901 tgtccgttga attccagccc atgaatatta aagaatgacc atggcaattg gatgtcattc
    63961 atgtgatgtg cataaaacat gccttgatag gccaggttgt gcacagtaaa caccgacttc
    64021 gccggacgcc cgcgcgccgc cagatacgca ggcgcaaggc ctgcatgcca gtcgtgcgca
    64081 tgcaccacat caggacgcca gaatgggtca agcccgctgg ccatttctgc cccaacccac
    64141 cccagcagcg caaaacgcaa tacgttgtcg gtataggcaa ataagttggt atcgtgatac
    64201 gggcttcccg gacgatcata gagatgcggc gcgtcaatca ggtatatgcc aaccccgttg
    64261 taatgaccga acaacagcgt gatatgcccg gcgaaggtat cacgacggga tactacctgc
    64321 gcatcggtca cgccacggcg aatatcggga aatgcaggca acagtacgcg agcgtcaacg
    64381 ccgtctgcga tttgtgctgc gggtaatgcc ccaataacat cagccagacc gccggtttta
    64441 agcagcggga acatctctga acatacatgt aaaacctgca ttatcgctcc tgtttatgcc
    64501 ctaacttccg tagcatttcg cgcgttacca gcacgatgcc ttcttctgaa cgatagaaac
    64561 gacgtgcatc ttcctctgcg ttttcaccaa tcaccatgcc ttccggaata acacaagcac
    64621 gatcgatgac gcagcggcgc agacggcacg agcgacctac ccatacttcc ggtaacaata
    64681 cggcggaatc aatgttgcag aatgaattca cgcgaacgcg cgagaacaga acggactgca
    64741 ccaccaccga accggagatc acacaaccgc cggaaaccag tgagttaagg gtcatcccgt
    64801 ggctaccgga gcgatcctgc acgaatttcg ctggcggtaa tgattcattg taggtgcgaa
    64861 ttggccaatt gcgatcgtac atatccagtt ccggcaccac agaggccaga tcgaggttcg
    64921 ctttccagta agcttccagc gtacccacat cgcgccagta cggctcggca tccggatcgg
    64981 attgtacgca agagagcggg aacgggtgcg cataggccag accggcttcg gtgatcttgg
    65041 gaatcaaatc tttgccaaag tcgtggctgg agttctcatc gcgatcgtct tcttccagca
    65101 gttcatacag atagtcggcg tcaaagacgt agatacccat actcgccaga gatttgctcg
    65161 gatcgttcgg cattgacggc gggttagcag gtttttcaac gaattcgata attttatcgt
    65221 tctcatcaac cgccataacg ccaaatgcgg aggcttcttc aatcggtact ggcatacaag
    65281 caacggtgca acgtgcgcct ttttcgacgt gatcgataag catacgcgag tagtcttgct
    65341 tgtagatatg gtcgcccgcc aggatcacca cgtattccgc tttataacgg cgaataatat
    65401 cgaggttttg ggtgaccgca tctgcggtgc cgcgatacca gttttcccct ttcattctct
    65461 gctgtgctgg cagcagatcg acaaactcgt tcatttcttc attgaagaat gaccagccgc
    65521 gctgaatgtg ctgcaccaga gtgtgggact ggtactgggt gatcacgccc atacgacgga
    65581 tcccggagtt gatgcagtta gacagcgcaa agtcgataat gcggaactta ccgccgaagt
    65641 gtacggccgg ttttgctcgc ttattggtta aatccttcag gcgggtacca cgtcctcccg
    65701 ccagtatcag ggcaacagat ttcaatggca gctggcgcgc caacattaag tgatcgttct
    65761 tctctaaact aaccatgact aactcctttt ttatcatctc tggaacacac acaatccgtg
    65821 tgcaggtccc tgccagacag ccgtaatcac tgggttatcc tctccagcga atgggggaat
    65881 ggcgtgccac tccccagcag gtaaaacaat ctctgttacc tcaagcgtgg cgttaattgc
    65941 gatcaaaaag cgatccgaga gcagaatttg cagctgtttc ggcccgtttt gccactcatc
    66001 cgtgcttaaa ggttgagcat atcgatttag ccaacggaca ttgccgtcgc cttcttccca
    66061 ccagcgattc tccaccaaag cgggaatacg cttgcgcaga tggattaacg cggcggtaaa
    66121 tgcggttaaa ccactgcttg cctgcgacca gtccaaccag gttaattggt tatcctgaca
    66181 gtaggcattg ttattgccat gctggctgtg accatgttcg tcaccggcca gtaacatcgg
    66241 cgtaccctgg gagagcaaca acgttgttaa cagggcgtga atgctgtcgc gccgccgttc
    66301 aaccaggtca agggtaccgc ctaccccttc tttaccatga ttgttactgt aattgttgtt
    66361 ggtcccgtcg cgattttctt ctccgtttgc ttcattgtgt ttatggttga aacaaacgca
    66421 gtcgcgaagc gtaaaaccgt catgcgcggt gacgagatta atcgcggcac tcggcagacg
    66481 accattacgt ttaaaaacat cgctggaggc agcaaaacgc ccggcaaacg cccccagagg
    66541 caaatcataa tgtagccaga aacgacgggc agcatcgcgg aaatgatcgt tccactcggc
    66601 aaacagcggc gggaaatttc ccacctgata accaccagga gcgatatccc acggttcagc
    66661 aattaacttc acctgcgaga gcaccgggca gttctggata gcggtaaaca acggcgcatc
    66721 ctgacggaac tctggcgtac ggcccatgac tgccgccaga tcaaagcgga aaccatcgac
    66781 gtggcaggtt tctacccaat aacgcaggca ggcgctggca taatccacca ccgccgggtg
    66841 actcaaattg agcgtgttgc cacaaccggt ccagttgtga taatcgccgt cttctcttat
    66901 ccaataatag ctacggttgt cgatcccacg cagcgagaat aacgggccgt cgaggtccag
    66961 ttccgcacta tggttgagca cgatatcaag aatgacttcg atacccgctt tatgcagtgc
    67021 tttgattgca tcgcgaaact catgcagcgc cgtttctggc gagcaggcat acgccggatg
    67081 cagcgcaaac atcgccaccg ggttgtaacc ccagtagtta cttagcccca tgcgttgcag
    67141 acgtggttca ctggcaaact gcgccactgg cagcagttcc agcgcggtaa tgcccaattg
    67201 tttcaaatag ttgatcatca ccggatgccc gagggcttta taagtgccac ggatctcgac
    67261 cgggatctcc gggtgcaggt acgttaatcc tttaacatgg gcttcataaa tgatggtgct
    67321 gccccacggc gtgcgcggcg gggcatcatc ttcccagtca tagtgatcaa ccaccactac
    67381 gcatttcggc gcaatggcgg cgttgtcgcg atagtcaggt tcattatgac cggcgtgcag
    67441 cagcgggtta tctttaaact ccccgtcaat ttgccgcgcg caaggatcaa tcaacaactt
    67501 cgccgggtta aagcgatgcc cctcggcggg ttgccagggg ccatgaacgc gataaccata
    67561 acgcaaaccc gggcgcgcat ccggcagata accgtgccaa atgtcgccac tgtgccctgg
    67621 caagtcatag cgatgttcct ggccattggc gtcaaagaca cacagttcta cccgctcggc
    67681 atgagcggag aaaagtgtga agttgacgcc ctgaccgtcg taatgcgcgc cgaggggagc
    67741 gggtttgcca atggcgagtt gtgtcattct gcctcccgaa ccagccagat agtggccagc
    67801 ggtggtagcg tcaggcttag tgaatgctga cgaccgtggc tggcaatctc atcgctgtgt
    67861 accgtgccgc cattacctgc attactgccg tgatagtgca tggaatcggt attgaggatc
    67921 tcacgccatt tgcctggctg attaatgccg aagcgataat catgacgcgg caccggcgta
    67981 aagttactgg caacgatgat ttcgttaccc tctttatcgc gacgcacaaa gatcagcacc
    68041 gagcgttctt tgtcatccac caccagccat tcaaagccgt acgggtcaaa atccagttca
    68101 tgcatcgctt tatggtggcg gtaggtgagg ttcagatcgc gcaccagacg ctggacaccg
    68161 tggtgccagt tatcgccgcc ttccaacaga tgccagtcga ggctggcgtc atggttccac
    68221 tcgcggccct gggcaaattc gttccccatg aacagcagtt tcttgcccgg aaatgcccac
    68281 atccagccat agtaagcgcg caggttagcg aatttctgcc atgcgtcgcc cggcatgcgg
    68341 tcgagaatcg attttttacc gtggaccact tcatcatgcg acaacggcag gacgaagttt
    68401 tcagtgtagt tgtagagcat cccgaaggtc agtttatcgt gatgatactg acgataaatc
    68461 gggtcgagct tcatgtagtc cagggtgtca tgcatccagc cgaggttcca cttgtaccag
    68521 aagcccagac cgcccatatc ctgcggacga gaaacgccag ggaaatcggt agactcctca
    68581 gccattgtca ccgcaccgga aacctgctca ccaagaatac ggttggtatt acgcaagaat
    68641 tcaatcgctt caagattctc gcgcccgcca aattcgttcg ggatccactc cccctcttta
    68701 cggctgtagt cacgataaat catcgacgcc accgcatcga cgcgcagcgc atcaatacca
    68761 aaacgttcaa tccagtaaag cgcgttaccg acgaggaagt tactgacttc acggcgacca
    68821 tagttgtaaa tcagcgtgtt ccagtcctga tgatagcctt cgcgcggatc gctgtgttca
    68881 tacaagttcg taccatcaaa ttcggcaagc gcaaagtcat cagtcgggaa gtggcctggc
    68941 acccagtcga gaatcacgtt cagaccagct gcgtgtgcag catcaatgaa ataacggaag
    69001 tcgtcgcgag taccaaaacg gcgggttggt gcatacaggc cggttggctg ataaccccaa
    69061 ctgccatcga agggatgctc gttaaggggc agtagttcga ggtgggtaaa gcccatccat
    69121 ttagcataag gcaccagttg atcggccagc tcgcggtagc tcaaccagaa attgttgtcg
    69181 gtgtggcgac gccaggagcc caggtgaact tcataaatag agattggcgc atcaaactga
    69241 ttcgcttttt tgcgctcttc agtctgtaca accttttccg gcagcccgca aataagagac
    69301 gcggtttccg ggcgcatttg cgcttcgaag gcataagggt cggacttcag acgcaagttg
    69361 ccattggcat caatcatctc gtatttatag agctgaccgt tatgcgcccc agggataaac
    69421 agttcccaga tgccgctctc tttacgcagg cgcatcgggt gacggcgacc gtcccagtag
    69481 ttgaattgcc caaccaccga gacccgacgg gcgtttggag cccagacaga gaaacgcgta
    69541 cctgtgacgc catccatagt atctgcatgc gcgcctaaag tttcatacgg gcgcaggtga
    69601 gtaccttcag ataatagcca ggcatccatt tcctggatta gcggaccaaa acggtaagga
    69661 tcatcaatca ggttttgctg cccatgccag acaacagcca actgatagcg gaaaaaattc
    69721 ttacgtcgcg gaatgacgcc actaaagaat ccccgtgagt cgagacactc cagttttgcg
    69781 agtttgcgcc cggttttcgg ttcaatcacc cacacatcgg tagcgtcggg taaaagggca
    69841 cggacttcca gtcccgcggt ggttttatgc attcccagta cggaaaaagg atccgcaaaa
    69901 tggcctgcaa ttagcgcgtt aatcacgtct ctatcgatac gatcggacat gcttgtcttc
    69961 ctgttttatt gtgtcacccc atccaattgg atttttgact tctggttgtg acattttttt
    70021 gacctgaacg gcgcagcact ctgtgcatcc tctctgcgtc gtcctcactt caggtaaggc
    70081 tgtgaatact catgtattca gccaccctta aagaatagcc aatgctctat ttaactcccg
    70141 gtaaatcatg aaacatctgc gcttactcct gtattacgca ctaacagggg cggcatcgcg
    70201 ccccagattt aatgaataaa gattacgcca gttgacgaag catccgacgc agcggctccg
    70261 cggcccccca cagcagctgg tcgcccacgg taaaggctga caggaactct ggtcccatat
    70321 tcagcttacg caggcggcct accggcgtgg tcagcgtgcc ggtaacggca gctggggtta
    70381 gctcacgcat agtgatttcc cgatcgttcg gaacgacttt cgcccacgga ttgtgcgcag
    70441 ccagcagttc ttccacggtc ggaatcgaca catctttttt caatttaata gtgaatgcct
    70501 ggctgtggca gcgcaatgcc ccgacacgca cacataaacc atctaccgga attacggaag
    70561 atgtgttgag gatcttgttg gtttccgcct gccctttcca ctcttcgcgg ctctgaccgt
    70621 tatcgagctg tttgtcgatc cacggaatca ggctacccgc cagcggcacg ccaaagttat
    70681 ccaccggcag ctcaccgcta cgggttaagg ttgtgacttt gcgttcgata tcgagaatag
    70741 cagaggacgg ggtcgcgagt tcatctgcta catggccata cagatggccc atctgggtta
    70801 ataactcacg catatgccgc gcaccaccgc cggaagcggc ctggtaggtt gcaacggaca
    70861 cccaatcaac aagatcattg gcgaataaac cacccaacga catcaacatc aggcttacgg
    70921 tacagttacc gccaacaaaa gtcctgatgc cattatttaa tccgtcggta atgacgtcct
    70981 gattgacggg gtcaagaatg atgatggcgt catctttcat gcgcagagac gatgctgcgt
    71041 caatccagta accctgccat ccgctttcac gaagctttgg atagatttcg ttggtataat
    71101 cgccgccctg acaggtcaca atgatatcga gggcctttag cgcctccaga tcaaaggcat
    71161 cctgaagtgt gccagtggtt ccgccaaaag acggcgcagc ctgaccaagc tgagaagtag
    71221 aaaagaagac agggcgaatg gcgtcgaagt cgcgctcttc aaccatgcgt tgcatgagaa
    71281 cggagccgac cataccgcgc cagccgataa aaccaacatt tttcataagc gttttttcct
    71341 gcaaagatgt gtgctgtata aatgtgccgg tctcctcttg gcacatcttt caccatacaa
    71401 aaagcagcca aagtcgcaag tgaaattaat caatgatagc gaagccatca gtaatgcgac
    71461 ttatcctgct ttgttagcac gcagaaagtc cgcggcaatt atcagggaat ttgagttatg
    71521 aatgaaatca tttctgcagc agttttattg atcctgatta tggatccgct cggaaaccta
    71581 cctattttca tgtccgtact gaaacatact gaaccgaaaa gacggcgggc aatcatggtg
    71641 cgagagttgc ttattgctct cctggtgatg ctggtgttcc tgtttgcggg tgagaaaatt
    71701 ctggcatttc tcagcctgcg agcagaaacc gtctccattt ctggcggcat cattctgttt
    71761 ctaattgcca ttaagatgat tttccccagc gcttcaggaa atagcagcgg gcttccggca
    71821 ggtgaagagc catttatcgt gccgctggca attccgctgg tcgccgggcc aactattctc
    71881 gccacgctga tgttgttgtc tcatcagtac ccgaatcaga tgggacatct ggtgattgct
    71941 ctgctgctgg cctggggcgg cacctttgtc atcctgctac agtcttcgct atttttacgt
    72001 ctgctgggcg agaaaggggt gaacgcactt gaacgcctga tgggattgat tctggtgatg
    72061 atggcaaccc agatgttcct cgacggcatt cgaatgtgga tgaaggggta aggttcaaac
    72121 aggtgctgca cacaaccaga cgcggcacct gttttattac ctgaactgat aatgaccttc
    72181 gcccaatcgg ataacccttc cctcttcttc tgcgcggttc agccacacac taatttgttc
    72241 gatggttaaa ccagtacaag acgccagcct ttcaatactt atcgattcct ttgcaaggat
    72301 cgccagttcc gccacaaaga gttgataaaa atcgacaggc gtatacgcgg gcattactgc
    72361 gtaatttgcg tcatcttcat gcagcgaaaa cagttctggt tgagcgttac ggagtgctct
    72421 gtcatacatc agcccaggaa cgtctggcag tctgatcgtc acattctcag cctgttcagc
    72481 actccatgac gccccatttt ctactaatcg tgaattggct gattgcatat cctgatttgg
    72541 tttaacctgc acaggcagcc actgatgttt taatgcctcc atcgccccgg ttatcgttcc
    72601 accggtcatt cccgcacgta caaccattgc gctttcagca aggcaataaa tatatttatt
    72661 tcgcgccatc gcattgccga cggtgaaacg tacctctggg taaaacggcg aaatcaacac
    72721 caggttgcct gctataagcc cttcacgcca tttcactaac gtactcgttt ttaacaagct
    72781 atcagctaat acgccaacgg cagttccccc ggcctccagt gccgacgcca ttgcacattc
    72841 atcaatacct cgcgcaccac cagagataac gcaaatcccc tgttgggcca gtttcgcggc
    72901 cagttgttgg gtatagcgca aatcgtcagt cggagcatct cgcgagccaa caatcgccat
    72961 accttccgct tgcagtaatg ctttattccc gcagccaaat aaaacgggag gtgcatccgt
    73021 tcgcaatcgg tttttcaagc gaacgggata atcagcatct ccgcgggtta agatccataa
    73081 accggcgcgt tgccacttat ctaccgccaa cgccagacta tgaccacgcg ccagcaagcc
    73141 aagaatacgc tcctgagaaa tacgcggatc gctccagcct gtaagtttct cttgcggttg
    73201 cggcaccaga agctcggcgg gactgatacg ttggtgcttc agccataatg caaacttccc
    73261 ccattcacta ttactaagag gtttatactt actcgccgcc gcgcgagaaa aatcgctggt
    73321 tagcaggaga gttgcttgtg cattggctga aagattcatc aattttttac cgaggtagaa
    73381 gcaagggcaa gaggataaac cgtcgggcaa cctgcctggc gtaacagtgc tgttagaact
    73441 gtcagcgtcc atgcagaatc cacgatatcg tcaaccagca acgccggacc aggcatcaaa
    73501 ggaggggtaa tcacaaacgc cccgtcgaga ttttgacact ggtggaaacg gttttgctgc
    73561 attttctgcg gtggattgtc cacgactttt tcaatggcat caataaaagg taagccaagt
    73621 ttcgccgcca gtcgccgggc aaaatcagga accaggtcga ggtgctttca aaatcaggaa
    73681 ccaggtcgag gtgctttaat gaaggaacgc agcataccca ggttggttca ggatgcggat
    73741 tccagcgttg gcgaaccatt tccgcacatg cctccaccag ttcatcactg aatcgccccg
    73801 tttttttctc ctgtgctacc tgctttcccc acccggaata tccccaacga gaaaggattc
    73861 gccccgtaga tccttgtaaa ctcgcaggca agttcccttt aaatccgtat tgcgtaaaag
    73921 ccccagctgc cacctgttta ttgagattta atgacaggtc agcgtgttta ataaataagt
    73981 ttgccgcatg gagtaagccg ctatcaatgt cgggcgacaa caacggatgc tgaagacaac
    74041 tgctacattt accacaatgt tcggcacctg gctcatcgag tgcgcggcgc agaaattgca
    74101 ttttgcactc cttggtcgtg atatagcttt caacgtctgc cagctcactt tttctctgat
    74161 tcatcaaatg gttaattcgt tcatggtcag gagaaaaact gacaattgtt ctgcgccata
    74221 atttctcggt atacaccaca ggcgatggat tttcagctac caataatttc agtgcttttt
    74281 ctatttgccc ataacggaga ttcgtccgtt gttcaatgcc tcgtaaggta agaccgtcat
    74341 tcacgctaag tacgttgagt atttcatgaa tttgcgcctc cgcaggaaag gcgctttcac
    74401 ggaagaattt atgaatagca cggtcttcac tgccgcaaag cagtatgcca acagcagaat
    74461 cgataccgcg accggcacgc cccacctgtt agtagtaacc aactatcgaa cctggcatct
    74521 ggtaatgaat aacaaacccc aaatccggtt tgtcaaagcc cattcccagc gccgttgtcg
    74581 caaccagcac tttaatttta tttgccagca acgcctgctc cagatattcc ctggcagtat
    74641 tagaatcttc cgcgccttca caagtcacgc cgctgtagta agcaaatgca ctgataccat
    74701 ttttccttaa ccactcggcg acaagttcgg catcgcgagt agtcagggta taaacaattc
    74761 ccgatttgga aaactgaggg ataaccgttg ctaaccatgc cagacgggat gagtgttcac
    74821 ctaagactaa ggcatcaagc gccaaacttt cgcgagccag cgttccacgc tgaatcacaa
    74881 tgtcacccaa ttgctgacgg atatcctcaa cgacacggtt attcgctgtc gcggttgtac
    74941 ccagaacagg ggtattcgca ggtagttgcg caaaatatct aatatacgtc ggtaatccgg
    75001 gcgaaaatcg tggccccaat cagagatgca gtgcgcctcg tcgaccacaa gcaagccaat
    75061 atgatcggct ataggatata aaaccgtttc gataaagtcc tggtttgcca aacgttccgg
    75121 ggaaaccagc aagcaatcga cctcaccttg cagcaactta tcgctaatgc gctgccattc
    75181 ttctctgttt gtagaattca acgtttcagc cgtaatacct aaccgttcag cggcggcaac
    75241 ctggttacgc atcagggcaa gcaaaggaga gatgataatt gtcgggccag cgccgcgatc
    75301 gcggaagatt ttactggcga tgaagtacac agcacttttc ccccagccag tgcgttggac
    75361 aaccagcagt tttcgccgtt ggttcaccac cgcgtctatg gcttcccatt gcccttccct
    75421 gaaagtagcg gtggtatttc ctaacatccg ctgtaaaagc aattcagctg cgtgcttttc
    75481 catctttatc tcctgcgatc tttaggcagt gattagaacg gattatttgc aggtaaattt
    75541 gcgagagaga tcaaaaagat gaagagagga gaaaagagaa atgaattact gcgagcaagc
    75601 tcgcagtaat ttgtcgcagt aattaactca acagctggaa cgcaatcatc ccaacgatgg
    75661 caccgacagt gccgaggatg gtttccatca tggtccaggt tttcagcgtt tcggcttcgg
    75721 tcgcgccggt aaatttaccg aacaaccaga aaccagcgtc gttaacgtgg ctgacaacaa
    75781 tcgaaccacc ggcgatacaa atcgacagcg ccgccatttg cgcaccggag tagttcagtt
    75841 gttcaataac cggcatcacc agtcccaccg ccgttaaaca ggcaacggtt gcagaaccct
    75901 gaatgatgcg cactgcagcg gccagcacga agcaggtgat ggcaatcggc aggcccatgc
    75961 cggttaacgc ttcgcccagt gccggaccta cgccagagtc aaccagcacc tgtttgaaca
    76021 cgccgcccgc accaatcacc agcagaatga tccccgccgg ttgcagcgcg tgaccgcaaa
    76081 tctccatcac tttgtctttt ggcatgccct gacgcattgc caggccataa atcgctacca
    76141 gacaagcaac cagaatcgcg gtaaacagat gaccaataaa ctcgaaccat tcgtaagcgg
    76201 tagagccttc cggcacaaaa cgcgcggcaa tggttttcag ccctaccagc accagcggca
    76261 gcaggatcag cgacaggctg aatccgaagg atggcatttt gccttcgccg agatgcggtt
    76321 cgctgatgtc gtcaggaata tgcagctcaa cgtaacggct gatgaagtta ccccacagcg
    76381 gcccggcaat aatcattccc ggaattgccg cacacaggcc aatcaggatc atccagccaa
    76441 aatcggcgtt catctgcgat gccagcagca ttggcgctgg ccctggcacc aggaacgccg
    76501 ccgcggccgc cacgcctgcg aataatggga ttaccagctt caccaggttc gtaccggtgt
    76561 ggcgcgccat tgagaaagca acgctaatca gcagaacaat cgccacttca aagaacagcg
    76621 gtagcgcaca gaccagcccc gcaaggccga tggcataatg cgcgcggctg tgaccgaagg
    76681 atttaagcat tttgacggca atctgatcga ctgcgccggt ttcatgtaag atcttgccaa
    76741 acatagctcc cagggcgaca accaccgcca ggaagccgag ggtgcctccc atcccttttt
    76801 ccatcgtcgc tgcgatttta tcgagcggca taccggaaaa aaggccagcc cccatggaca
    76861 ccaccattaa agccaggaaa gcgtgcatac gcgccttcat gactaaaaac agcagcagta
    76921 aaacagaccc tactgctgtt aaaacaagcg ttaatgtagt cactacttat ttgccttttt
    76981 taataacctc aatggtgctt gccacaacac cttccagcgg ttgatcgata tccaccacca
    77041 gtacatcggt ttcgtccgca cccggctcct gcagcgtttc aaactgcgtc accaacattt
    77101 gggttttaaa gaaatggcct ttgcgcgctt tcaggcggct ttcaatcaca tcaaaatcgc
    77161 ctttcaggta gatgaaagag agattcggat taccttcacg cagcaagtca cgatagtgtt
    77221 ttttcaatgc agaacagacg atcagcgaca ctttattagt gcgctgcata gcaaacgcgg
    77281 cgtcgttcag cgcctgcaac cacggtttgc gatcgtcgtc attcagcggt tcgccagacg
    77341 ccattttttc gatattgcgc cgtggatgga ggaaatcgcc atcaagaaac gcggcatgaa
    77401 gttgatgcgc cacttcactt gcgaccgcag atttgccgct gcccgatacg cccatcaaga
    77461 cgtaaatgtg gtgatcatgg ttagtcgtgc tcaaagtggt gcccccacaa tacaagaatt
    77521 aaaaatgtta cgggtaactg ttatcggtaa cattgtccag ccggacaata tcagaagcaa
    77581 tatccatccg tgccttaagt gtataagtgt gagctacttc aaatttgtgg gcttaaatag
    77641 atccgcccgg tgacaaggtg aaacctaaat ctaacatttt cggtgtcaca gattcgccac
    77701 gaatacgcgc cagcaggcgt tcagcgccaa tactgcccat ccgctcacgc ggcgtcagca
    77761 cgctcgcaag tcgtggctcc atcacctgac caatgtcatg accgtggaaa ccggcaatcg
    77821 ccatatcgtc aggaactttt aaccccagac gctgacattc aaacgccgcg ccgaccgcca
    77881 gatcatcatt agtacagaac acgccatcca gctgcggata ttcccgccgt gcctggcgaa
    77941 tcagttcaat accggaagag taagaagaag attgctcaac catcacgcta tatggcacca
    78001 ggcctgcatc cagcatcgcc tgttcgtatc ccttctgttt gatgatagta cgttcgtcga
    78061 gacgtgcgcc gagataggca atgtggcgat gcccgcgagc aataatggct gtggtcatct
    78121 ggcgtgccgc ttcaaagtta tcaaaaccga cggcgatatc gagacacggc gactggctgt
    78181 ccatcaactc caccaccggg atccccgcca cttcaatcat ctttaaggtg cgcggcgtgt
    78241 gggtacgttc ggtgaggatc agtccgtcga tattccatga gagcattgat tcgagacgtt
    78301 cttgctccat ttccggttta taaccgtagt gggccagcat ggtctgataa ccgtgcgcgt
    78361 cggtgacgct ttcgattccg cgtaatactt ccgcgaaaac ctggttggtg agagaaggta
    78421 acaggacgcc aatcgcccgg ctggtggcgt tagagaggat atcgggcgca cgattgggaa
    78481 tatagcccag ttcatcaaga gccgcggcaa tcttgccgcg tagagcgacg gaaacctgct
    78541 ccgggttgcg taaaaaacgg ctgaccgtca ttttggtcac gcctacacgg tcagccacat
    78601 cctgaagtac gggtcttttc tttttcatcg tcctgaaggt acaaaagaga tagattctct
    78661 tagtttaaca cggacgcatc agaaccttcc cggggaaaac agtgcttgtt tacaattatt
    78721 agatatacac taaatcaatt gcatcgcatt gtgctaacga cgctgcaaaa ccccggaagc
    78781 gtacatcagc acgcgaccgg agcgagcgag gaagcctgca aaaaggcagg cttcaaagat
    78841 gccgttgagc tttaaaccgg cggcagatcg aacagtaaca cttcgctgtc gctatccgca
    78901 tggatggaga ttgcctgctc atcccagatt gccagaccat cgctgatcga ggctttcacg
    78961 ccgttaatgg tgacattgcc tttcaccacc tggatccaga cgcggcgttc agcggcaatc
    79021 tgatgcaccg actgctcatc tttcagcaac gcccagcggt acagttccat atcctgatgc
    79081 actttcaatg aaccatcacg cgcatctggc gaaagcacca gttgtttgcc ctgcactgca
    79141 tcgaagcgac gctgttcata acgcggcgta atgccgtttt cttccggcat gatccagatc
    79201 tgatacaggt gcagacgctc ggtgctgctt gggttgtact ctgagtgacg aatacccgta
    79261 cccgcactca taatctggaa ctcgcccgcc ggaacctgct ctttattgcc catgctgtcc
    79321 tggtgctcaa cagtaccttc cagcacgtag gtcaaaattt ccatatcttt atgcgggtga
    79381 gtgccgaagc cctgccctgc ttcaatcacg tcgtcgttaa tcacgcgcag cgcggagaag
    79441 cccataaagt tcggatcgta atagttggca aaagagaaag tatgccagga gtccagccag
    79501 ccatgatttg catgaccacg ttcatttgct ttgcgtaagt agatcatttg tattcgcccc
    79561 ctgaatgatt tcgatgggct tagtgtggac ccgatctgcc tgggatgata gagggtgaaa
    79621 attgacccct ctgttcaaaa aaattgaact attcgagggg ccatcaaact tacttcgcga
    79681 gagtcactgt ggagggagag gcttgctcga atccacgttc aagtatttcc agattcgtaa
    79741 gaacttcaga ttccttgacg taatttggcg caccgtgggt gatggtttga tacaacgcat
    79801 cataaacgcg cccgtaatcg cccatctccg gcttcatctc ttctctgacc gtcacgccct
    79861 cgtcattgac atactccagc acaccgaccg aatcatccgc tgcgaatccc ggttcgcccg
    79921 gcataatatt agccttcagg ctggtttcct gctggtcgat accatattta ataaacgaac
    79981 ctttcttacc gtgaacgata aatttcggat aatcgatttt caccagatgg ctggttttga
    80041 cgatggcttt tagatcgcca taaaacagtt gcgcttcgaa agtatcgtca ggattggctt
    80101 tattgcgcag gctgcggatg tcataagcga cgtgatccgg gcgaccgaac agagaaataa
    80161 tctggtccat cgtatgcaca ccaaggccat agaacgcgcc atcctgcggc agcccaggtt
    80221 tggtttctgc caccgggcgg taatagtcaa aatggctttc cacttcgaca atctctccca
    80281 gcttgccgct ttcaatcgct tttttcgccg tcaggaagca ggaatcaaag cgacgattct
    80341 gatatggcgt gacggtcagc cctttgcttt tcgccagtgc aaacagctct ttcgcctgcg
    80401 caagtgtcgg agtgaacggt ttttcgacca gcacattttt cccggcttcc atcgcgcgct
    80461 tcgcgtactc gaagtggctg tccgcatggg tgcagacaac aaccagctta acatcgggat
    80521 cgtttagcac ttcgttgaga tcgctggtga aatggatatg agagtaaatg ggagcttgtt
    80581 cttccggctt tgcatggcgg cgaaaaatat gcgcgacatg ccagctatcc ttgcggttaa
    80641 gtacatacgg cagatggtaa cgggtggtgc ttttgccgaa gccaataaag gcgcagttga
    80701 tgaccatgat ttcgtccttt ttaaggtggt tatttacacc ttagcgcaaa gcggacgtgg
    80761 ttcctactgg agtgcgcata acgaacacaa gcacttcccg aggataaatt gagaacgaaa
    80821 gatcaaaaaa aaagccagca cccggctggc taagtaatac tggaagcaat gtgagcaatg
    80881 tcgtgctttc aggttctccg cgagggtctt cctgatcgcg agacaataat aatcattctc
    80941 attcgcactt gtccaacact ttttgcaaaa aaatgcattt gactcgcatt cgaaagtcaa
    81001 tgatgttgaa agggacattt accccaaaga ggacaaagga atgagtgaga tagtgatacg
    81061 ccatgctgaa acacgggatt acgaggccat caggcagatt cacgcccagc cggaggtgta
    81121 ttacaacaca ctacaggtgc ctcatccttc cgatcatatg tggcaggagc gactcgccga
    81181 tcgtcccggc atcaagcaac tcgtcgcctg tattgatgga gacgtcgtgg gccatctcac
    81241 cattgacgtg caacagcgcc cacgccgcag tcatgttgcc gattttggta tctgtgtcga
    81301 cgcccgctgg aagaatcgcg gcgtcgccag cgccctgatg cgagagatga ttgaaatgtg
    81361 cgacaactgg ttgcgggtag atcgtattga gctaacggtg ttcgtcgata acgcgccagc
    81421 aattaaggtc tataaaaaat tcggctttga gattgaaggg actggcaaga agtacgcatt
    81481 acgtaatggt gaatatgttg atgcgtattt tatggcgcgg gtgaagtaaa gaatattctc
    81541 aaattgcctg ttaccaatgt gaataattag ttttgttcat ctaatttgag tttttcctta
    81601 tattaccacc atcattttga aacaatttgt aacataaaga gacctttgaa tcattactcg
    81661 ttaatatttc ttattaaaga gaacatattt tcgcaagcaa tagttattac tgtttgttta
    81721 tggatagaga gtctacttaa tgggtacagc agctataagt catttacgat atgatcttaa
    81781 caaatatgcc ctttctttac gcaaaaccgc aacacttgct tcaacatttt ttattgagtc
    81841 cccattagtt agattcgagt atctgcaaga a
//
LOCUS       DAZFAB010000010        79029 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZFAB010000010 DAZFAB010000000
VERSION     DAZFAB010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 79029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79029
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.010"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(90..1040)
                     /gene="dusC"
                     /locus_tag="ACHJWA_000818"
     CDS             complement(90..1040)
                     /gene="dusC"
                     /locus_tag="ACHJWA_000818"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFO4307470.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQECMAISGC
                     DSVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIGKLR"
     gene            complement(1200..2393)
                     /locus_tag="ACHJWA_000819"
     CDS             complement(1200..2393)
                     /locus_tag="ACHJWA_000819"
                     /EC_number="1.14.13.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybenzoate 6-monooxygenase"
                     /protein_id="HFO4307471.1"
                     /translation="MTKVTRAVIVGGGIGGAATALSLARLGIKVVLLEKAHEIGEIGA
                     GIQLGPNAFSALDSLGVGEVARQRAVFTDHITMMDAVNAEEVVCIETGQAFRDHFGGP
                     YAVIHRVDIHASVWEAVLTHPDVEYHTSTNVVDIRETEDDVTVFDDRGNSWTGDILIG
                     CDGVKSVVRQSLLGDAPRVTGHVVYRAVIDRADMPEDLRINAPVLWAGPHCHLVHYPL
                     RGGKQYNLVVTFHSRQQEEWGVKDGSKEEVLSYFEGIHPRPRQMLDKPTSWRRWSTAD
                     REPVAKWGTKRITLVGDSAHPVAQYMAQGACMALEDAVTLGKALAQCEGDAAQAFALY
                     ESVRIPRTARIVWSTREMGRVYHAAGVERQVRNLLWKGKSQAEFYRGMEWLYGWKEDN
                     CLQPR"
     gene            complement(2408..3052)
                     /gene="maiA"
                     /locus_tag="ACHJWA_000820"
     CDS             complement(2408..3052)
                     /gene="maiA"
                     /locus_tag="ACHJWA_000820"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HFO4307472.1"
                     /translation="MKLYSFFNSSASYRVRIALALKGINYQTEGVNIRIGQQNELAYR
                     RMNPVGLVPTLLTDEGESLGQSLAIIDWLERHYPQVPLVPQEEPARNKVLEIVYAIAC
                     DIHPLNNLRVLRYLTEELNVSEEEKKRWYAHWIQQGLSAVEQLLRQNQSGQFCVGETP
                     TLADCCLVPQWANALRMNCDLSGYPRCKAVYDACTQLPAFIAAAPENQQDKISA"
     gene            complement(3061..3762)
                     /locus_tag="ACHJWA_000821"
     CDS             complement(3061..3762)
                     /locus_tag="ACHJWA_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFO4307473.1"
                     /translation="MTQYVFEPQAPVTVPVVGSDEQFPVRRVYCVGRNYAAHAREMGF
                     DPDREPPFFFCKPADAVVPVAAGETLALPYPAQTDNYHYEIELVVAIGKKGSDIPLEH
                     AHEYVWGYATGLDMTRRDRQMEMRQMGRPWEIGKAFDLSAPIAPLHKARDIGGIDNAP
                     IWLQVNGEDHQRSDIRHLIWSVNETICYLSGFFELQPGDLIFTGTPEGVGPVVKGDVI
                     TGNVEGLTPIAVKIV"
     gene            complement(3777..4805)
                     /gene="gtdA"
                     /locus_tag="ACHJWA_000822"
     CDS             complement(3777..4805)
                     /gene="gtdA"
                     /locus_tag="ACHJWA_000822"
                     /EC_number="1.13.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047922 - gentisate 1,2-dioxygenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gentisate 1,2-dioxygenase"
                     /protein_id="HFO4307474.1"
                     /translation="MTDNNQNSREQFYQHISGQNLTPLWESLHHLVPKTPNANCAPAY
                     WNYQEIRPLLLESGGLIGAKEAVRRVLVLENPALRGQSSITATLYAGLQLIMPGEVAP
                     SHRHNQSALRFIVEGKGAFTAVDGERTPMNEGDFILTPQWRWHDHGNPGDEPVIWLDG
                     LDLPLVNILGCGFAEDYPEEQQPVTRKEGDYLPRYAANMLPLRHQTGNSSPIFNYRYD
                     RSREVLHDLTRLGDADEWDGYKMRYVNPVTGGYPMPSMGAFLQLLPKGFASRVARTTD
                     STIYHVVEGSGQVIIGNETFSFSAKDIFVVPTWHGVSFQTTQDSVLFSFSDRPVQEAL
                     GLFREARY"
     gene            complement(4817..6175)
                     /locus_tag="ACHJWA_000823"
     CDS             complement(4817..6175)
                     /locus_tag="ACHJWA_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4307475.1"
                     /translation="MTQRRDLQALIDAAPVGKMQWRVIICCFLVVMLDGFDTAAIGFI
                     APDIRTHWQLTAGDLAPLFGAGLLGLTAGALLCGPLSDRFGRKRVIELCVFLFGALSL
                     ASAFSPDLQTLVFLRFLTGLGLGGAMPNTITMTSEYLPARRRGALVTLMFCGFTLGSA
                     FGGIVSAQLVPVIGWHGILVLGGVLPLMLFVALLVVLPESPRWQVRRQLPQAVIAKTV
                     SAITRERYVDTHFYLIESASVTKGSIRQLFMGRQLPITLMLWVVFFMSLLIIYLLSSW
                     MPTLLNHRGIDLQHASWVTAAFQIGGTLGALALGVLMDKFNPFRVLTLSYAIGAICIV
                     MIGLSQDGLWLMALAIFGTGIGISGSQVGLNALTATLYPTQSRATGVSWSNAIGRCGA
                     IVGSLSGGVMMAMNFSFDTLFFIIAVPAAISAVMLTLLITVVRQSTSVPDSLPRAGVV
                     NE"
     gene            6302..7210
                     /locus_tag="ACHJWA_000824"
     CDS             6302..7210
                     /locus_tag="ACHJWA_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO4307476.1"
                     /translation="MANWAQKLKLHHLEMLVALGEQGNLTHVARMMNITQPALSKWLS
                     QLEDEIGITLFERHSKGLRPTEGGKLLLQHAQRLINDMERSQHELARFKQGGLVGSLK
                     IGCSPVATDCVSQAILHLLTKMPTLHLNIEEKVMTPLLQDLLAGSVDVVVGRIGGRAL
                     QLPLNYQVLYTEPVCFVARPEHPLAKYATLSWNDLANWRWIVWPTGTPIRISIDNALV
                     DHGVMLPENTIESASMNVSTNLLQSSDMISILSLRLAQRYASQGQLVILNLPKIEQKG
                     SVGVFWRNNETPSYALSQFLHFLQQN"
     gene            7341..7739
                     /locus_tag="ACHJWA_000825"
     CDS             7341..7739
                     /locus_tag="ACHJWA_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFO4307477.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAI
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            7736..8431
                     /locus_tag="ACHJWA_000826"
     CDS             7736..8431
                     /locus_tag="ACHJWA_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFO4307478.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APILFPIILAVMG"
     gene            8561..9445
                     /gene="cdd"
                     /locus_tag="ACHJWA_000827"
     CDS             8561..9445
                     /gene="cdd"
                     /locus_tag="ACHJWA_000827"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFO4307479.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LADAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHNIDRVLLA"
     gene            9595..10314
                     /gene="sanA"
                     /locus_tag="ACHJWA_000828"
     CDS             9595..10314
                     /gene="sanA"
                     /locus_tag="ACHJWA_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFO4307480.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            10317..10556
                     /locus_tag="ACHJWA_000829"
     CDS             10317..10556
                     /locus_tag="ACHJWA_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFO4307481.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            10750..11988
                     /gene="preT"
                     /locus_tag="ACHJWA_000830"
     CDS             10750..11988
                     /gene="preT"
                     /locus_tag="ACHJWA_000830"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFO4307482.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLLRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDDFAKLEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            11982..13217
                     /gene="preA"
                     /locus_tag="ACHJWA_000831"
     CDS             11982..13217
                     /gene="preA"
                     /locus_tag="ACHJWA_000831"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFO4307483.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(13460..14470)
                     /gene="mglC"
                     /locus_tag="ACHJWA_000832"
     CDS             complement(13460..14470)
                     /gene="mglC"
                     /locus_tag="ACHJWA_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFO4307484.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQAPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(14486..16006)
                     /gene="mglA"
                     /locus_tag="ACHJWA_000833"
     CDS             complement(14486..16006)
                     /gene="mglA"
                     /locus_tag="ACHJWA_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFO4307485.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYKKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKKINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(16067..17065)
                     /gene="mglB"
                     /locus_tag="ACHJWA_000834"
     CDS             complement(16067..17065)
                     /gene="mglB"
                     /locus_tag="ACHJWA_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFO4307486.1"
                     /translation="MNKKVLTLSAVMASMLFGATAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(17344..18384)
                     /gene="galS"
                     /locus_tag="ACHJWA_000835"
     CDS             complement(17344..18384)
                     /gene="galS"
                     /locus_tag="ACHJWA_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFO4307487.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYTHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            complement(18526..19683)
                     /gene="yeiB"
                     /locus_tag="ACHJWA_000836"
     CDS             complement(18526..19683)
                     /gene="yeiB"
                     /locus_tag="ACHJWA_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFO4307488.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVIINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMQFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGPAISKTSR"
     gene            complement(19700..20368)
                     /gene="folE"
                     /locus_tag="ACHJWA_000837"
     CDS             complement(19700..20368)
                     /gene="folE"
                     /locus_tag="ACHJWA_000837"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFO4307489.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            20626..21462
                     /gene="yeiG"
                     /locus_tag="ACHJWA_000838"
     CDS             20626..21462
                     /gene="yeiG"
                     /locus_tag="ACHJWA_000838"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO4307490.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSRYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(21494..23473)
                     /gene="cirA"
                     /locus_tag="ACHJWA_000839"
     CDS             complement(21494..23473)
                     /gene="cirA"
                     /locus_tag="ACHJWA_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFO4307491.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRNGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFTA
                     NGVPVFSYYNVNKARIQGVETELKIPFNDEWKLSLNYTYNDGRDVSNGENKPLSDLPF
                     HTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVTKDVK
                     LRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(23765..25234)
                     /gene="lysP"
                     /locus_tag="ACHJWA_000840"
     CDS             complement(23765..25234)
                     /gene="lysP"
                     /locus_tag="ACHJWA_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFO4307492.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPGKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(25439..26320)
                     /gene="yieE"
                     /locus_tag="ACHJWA_000841"
     CDS             complement(25439..26320)
                     /gene="yieE"
                     /locus_tag="ACHJWA_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFO4307493.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            26419..27468
                     /locus_tag="ACHJWA_000842"
     CDS             26419..27468
                     /locus_tag="ACHJWA_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFO4307494.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            27542..28399
                     /gene="nfo"
                     /locus_tag="ACHJWA_000843"
     CDS             27542..28399
                     /gene="nfo"
                     /locus_tag="ACHJWA_000843"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFO4307495.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARTVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            28402..29490
                     /locus_tag="ACHJWA_000844"
     CDS             28402..29490
                     /locus_tag="ACHJWA_000844"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO4307496.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGMADIRYPQSASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDET
                     VLAINDTHLLEQLSPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLAGEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(29546..30796)
                     /locus_tag="ACHJWA_000845"
     CDS             complement(29546..30796)
                     /locus_tag="ACHJWA_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFO4307497.1"
                     /translation="MDVMRSVLGMVVLLTIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESIVGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(30896..31837)
                     /gene="rihB"
                     /locus_tag="ACHJWA_000846"
     CDS             complement(30896..31837)
                     /gene="rihB"
                     /locus_tag="ACHJWA_000846"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFO4307498.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGDTGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDSDWFWGLVEECVRGYIKTH"
     gene            31967..32665
                     /locus_tag="ACHJWA_000847"
     CDS             31967..32665
                     /locus_tag="ACHJWA_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFO4307499.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(32736..33986)
                     /locus_tag="ACHJWA_000848"
     CDS             complement(32736..33986)
                     /locus_tag="ACHJWA_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFO4307500.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVAAALLLQIALGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATELSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(34080..34997)
                     /gene="psuG"
                     /locus_tag="ACHJWA_000849"
     CDS             complement(34080..34997)
                     /gene="psuG"
                     /locus_tag="ACHJWA_000849"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFO4307501.1"
                     /translation="MSELKISPEVQDALKNKKPVVALESTIISHGMPFPQNAQTAIEV
                     EETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFVVAAGKNG
                     ATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTVVCAGAKS
                     ILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFDVSIRLDSASEIARAMAVKWQS
                     GLNGGLVVANPIPEQFAMPEHTINAVIDQAVAEAEAQGVIGKESTPFLLARVAELTGG
                     DSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            complement(34985..35926)
                     /locus_tag="ACHJWA_000850"
     CDS             complement(34985..35926)
                     /locus_tag="ACHJWA_000850"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFO4307502.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRERLSQIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            complement(36350..38041)
                     /gene="fruA"
                     /locus_tag="ACHJWA_000851"
     CDS             complement(36350..38041)
                     /gene="fruA"
                     /locus_tag="ACHJWA_000851"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFO4307503.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATSE
                     GKNESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(38058..38996)
                     /gene="fruK"
                     /locus_tag="ACHJWA_000852"
     CDS             complement(38058..38996)
                     /gene="fruK"
                     /locus_tag="ACHJWA_000852"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFO4307504.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWSAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(38996..40126)
                     /gene="fruB"
                     /locus_tag="ACHJWA_000853"
     CDS             complement(38996..40126)
                     /gene="fruB"
                     /locus_tag="ACHJWA_000853"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFO4307505.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVVEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            40494..41675
                     /gene="setB"
                     /locus_tag="ACHJWA_000854"
     CDS             40494..41675
                     /gene="setB"
                     /locus_tag="ACHJWA_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFO4307506.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGVLVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(41672..41926)
                     /locus_tag="ACHJWA_000855"
     CDS             complement(41672..41926)
                     /locus_tag="ACHJWA_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO4307507.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            42081..42653
                     /gene="yeiP"
                     /locus_tag="ACHJWA_000856"
     CDS             42081..42653
                     /gene="yeiP"
                     /locus_tag="ACHJWA_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFO4307508.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            42876..44342
                     /locus_tag="ACHJWA_000857"
     CDS             42876..44342
                     /locus_tag="ACHJWA_000857"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFO4307509.1"
                     /translation="MNTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQAIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFSHISDCMQDRAFRHAARTLMLDE
                     QAPTLRIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRDLVAGSISEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQARQQIAQFGAHQALLNTLKI"
     gene            44460..45446
                     /gene="yeiR"
                     /locus_tag="ACHJWA_000858"
     CDS             44460..45446
                     /gene="yeiR"
                     /locus_tag="ACHJWA_000858"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFO4307510.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKNPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVAGHLLDLPRHNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGHQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            45485..46198
                     /gene="lpxT"
                     /locus_tag="ACHJWA_000859"
     CDS             45485..46198
                     /gene="lpxT"
                     /locus_tag="ACHJWA_000859"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFO4307511.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            46611..47177
                     /gene="mepS"
                     /locus_tag="ACHJWA_000860"
     CDS             46611..47177
                     /gene="mepS"
                     /locus_tag="ACHJWA_000860"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFO4307512.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            47358..48914
                     /locus_tag="ACHJWA_000861"
     CDS             47358..48914
                     /locus_tag="ACHJWA_000861"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO4307513.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFADLKSTTDRLQPLTLDTCKQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKANIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            48996..50810
                     /locus_tag="ACHJWA_000862"
     CDS             48996..50810
                     /locus_tag="ACHJWA_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFO4307514.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYSGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDHSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            50811..51905
                     /locus_tag="ACHJWA_000863"
     CDS             50811..51905
                     /locus_tag="ACHJWA_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFO4307515.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            51905..52930
                     /locus_tag="ACHJWA_000864"
     CDS             51905..52930
                     /locus_tag="ACHJWA_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4307516.1"
                     /translation="MSRLNPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            52932..54521
                     /gene="yejF"
                     /locus_tag="ACHJWA_000865"
     CDS             52932..54521
                     /gene="yejF"
                     /locus_tag="ACHJWA_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFO4307517.1"
                     /translation="MTQTLLAIEDLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNYAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIDVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(54525..54869)
                     /locus_tag="ACHJWA_000866"
     CDS             complement(54525..54869)
                     /locus_tag="ACHJWA_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFO4307518.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALGDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(55202..56392)
                     /gene="bcr"
                     /locus_tag="ACHJWA_000867"
     CDS             complement(55202..56392)
                     /gene="bcr"
                     /locus_tag="ACHJWA_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFO4307519.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(56420..57115)
                     /gene="rsuA"
                     /locus_tag="ACHJWA_000868"
     CDS             complement(56420..57115)
                     /gene="rsuA"
                     /locus_tag="ACHJWA_000868"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFO4307520.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            57264..59024
                     /locus_tag="ACHJWA_000869"
     CDS             57264..59024
                     /locus_tag="ACHJWA_000869"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO4307521.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAATRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            59149..59433
                     /gene="rplY"
                     /locus_tag="ACHJWA_000870"
     CDS             59149..59433
                     /gene="rplY"
                     /locus_tag="ACHJWA_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFO4307522.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNIQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(59572..60579)
                     /gene="yejK"
                     /locus_tag="ACHJWA_000871"
     CDS             complement(59572..60579)
                     /gene="yejK"
                     /locus_tag="ACHJWA_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFO4307523.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            60761..60988
                     /locus_tag="ACHJWA_000872"
     CDS             60761..60988
                     /locus_tag="ACHJWA_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFO4307524.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            61008..62768
                     /gene="yejM"
                     /locus_tag="ACHJWA_000873"
     CDS             61008..62768
                     /gene="yejM"
                     /locus_tag="ACHJWA_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFO4307525.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            62843..62919
                     /locus_tag="ACHJWA_000874"
     tRNA            62843..62919
                     /locus_tag="ACHJWA_000874"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:62877..62879,aa:Pro,seq:ggg)
     gene            complement(63020..65611)
                     /gene="yejO"
                     /locus_tag="ACHJWA_000875"
     CDS             complement(63020..65611)
                     /gene="yejO"
                     /locus_tag="ACHJWA_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HFO4307526.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVVLE
                     KDIQLVYGTANNTKINPGGEQHIKEFGISSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGAANDPTIKGGRLIVEKDGITVLAAIEKGGLLEVKEGG
                     LAIAVDQKAGGAIKASTRVMEVFGTNRLGQFEIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTRVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     LRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMDESVTRS
                     SVTASAENFTTLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTAMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNVDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTRGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            65931..66578
                     /gene="narP"
                     /locus_tag="ACHJWA_000876"
     CDS             65931..66578
                     /gene="narP"
                     /locus_tag="ACHJWA_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFO4307527.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLHEREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(66613..67665)
                     /gene="ccmH"
                     /locus_tag="ACHJWA_000877"
     CDS             complement(66613..67665)
                     /gene="ccmH"
                     /locus_tag="ACHJWA_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFO4307528.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDATLGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(67662..68219)
                     /gene="dsbE"
                     /locus_tag="ACHJWA_000878"
     CDS             complement(67662..68219)
                     /gene="dsbE"
                     /locus_tag="ACHJWA_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFO4307529.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(68216..70159)
                     /gene="ccmF"
                     /locus_tag="ACHJWA_000879"
     CDS             complement(68216..70159)
                     /gene="ccmF"
                     /locus_tag="ACHJWA_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFO4307530.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(70156..70635)
                     /gene="ccmE"
                     /locus_tag="ACHJWA_000880"
     CDS             complement(70156..70635)
                     /gene="ccmE"
                     /locus_tag="ACHJWA_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFO4307531.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(70632..70841)
                     /gene="ccmD"
                     /locus_tag="ACHJWA_000881"
     CDS             complement(70632..70841)
                     /gene="ccmD"
                     /locus_tag="ACHJWA_000881"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFO4307532.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(70838..71575)
                     /gene="ccmC"
                     /locus_tag="ACHJWA_000882"
     CDS             complement(70838..71575)
                     /gene="ccmC"
                     /locus_tag="ACHJWA_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFO4307533.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(71617..72279)
                     /gene="ccmB"
                     /locus_tag="ACHJWA_000883"
     CDS             complement(71617..72279)
                     /gene="ccmB"
                     /locus_tag="ACHJWA_000883"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFO4307534.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(72276..72899)
                     /gene="ccmA"
                     /locus_tag="ACHJWA_000884"
     CDS             complement(72276..72899)
                     /gene="ccmA"
                     /locus_tag="ACHJWA_000884"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFO4307535.1"
                     /translation="MGMLEVRELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAV"
     gene            complement(72912..73514)
                     /gene="napC"
                     /locus_tag="ACHJWA_000885"
     CDS             complement(72912..73514)
                     /gene="napC"
                     /locus_tag="ACHJWA_000885"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFO4307536.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(73524..73973)
                     /gene="napB"
                     /locus_tag="ACHJWA_000886"
     CDS             complement(73524..73973)
                     /gene="napB"
                     /locus_tag="ACHJWA_000886"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFO4307537.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(73970..74833)
                     /gene="napH"
                     /locus_tag="ACHJWA_000887"
     CDS             complement(73970..74833)
                     /gene="napH"
                     /locus_tag="ACHJWA_000887"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFO4307538.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(74820..75515)
                     /gene="napG"
                     /locus_tag="ACHJWA_000888"
     CDS             complement(74820..75515)
                     /gene="napG"
                     /locus_tag="ACHJWA_000888"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFO4307539.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLVVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(75522..78008)
                     /gene="napA"
                     /locus_tag="ACHJWA_000889"
     CDS             complement(75522..78008)
                     /gene="napA"
                     /locus_tag="ACHJWA_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFO4307540.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQDVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(78005..78268)
                     /gene="napD"
                     /locus_tag="ACHJWA_000890"
     CDS             complement(78005..78268)
                     /gene="napD"
                     /locus_tag="ACHJWA_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFO4307541.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(78258..78752)
                     /gene="napF"
                     /locus_tag="ACHJWA_000891"
     CDS             complement(78258..78752)
                     /gene="napF"
                     /locus_tag="ACHJWA_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFO4307542.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            78861..79025
                     /gene="yojO"
                     /locus_tag="ACHJWA_000892"
     CDS             78861..79025
                     /gene="yojO"
                     /locus_tag="ACHJWA_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HFO4307543.1"
                     /translation="MHTPIEVKPVAGSKEWREAWQKRAFAHISNGYKYIYIAINSPEI
                     FLLVCSLIRI"
BASE COUNT        19272 a        21331 c        19513 g        18913 t
ORIGIN      
        1 aatatgtggc ttagctcatg aaaggagcgc aacaagatga tgaagctgaa taagcgattc
       61 attgtttctg ataaactgcg attccactat tatcgcagct taccgatatc aattgcctga
      121 atagcccttg caatatcagg ggaattattc aacacacgaa catgctgaaa taattctgtt
      181 gcttcatcgt attctttacg caaataactc aaccactgtt taatccgcgc tacgtgatat
      241 aacccggtat cgccctgctt ttccagacgg gtatattttt gcagcaaagc aaccacctcc
      301 ggccacggca ttcgcggttc gttatatttt actacccggc tcaggttggg aatattgagc
      361 gccccgcgac caatcatcac tgagtcgcag ccgctgatcg ccatgcattc ttgcgcgctc
      421 tgccagtccc agatttcgcc gttggcaatc accggaatat tcagccgctg gcgaatctcg
      481 ccaatcgcct gccagtcaat atgctccgcg cggtaaccct gctcttttgt ccgtccatgc
      541 accaccagct ccgtagcacc agcctgttga accgcatcgg cgatttcaaa tttcttctca
      601 ccgctgtccc agcccagacg cactttcacg ctgacgggca aatgcgccgg tacagcttca
      661 cgcatcgctt ttgcaccctg gtagatgagt tcaggatctt tgagtaacgt cgccccacca
      721 ccgctaccgt taaccgtttt cgacgggcag ccgcaattga gatccacgcc ccaggaacct
      781 aattccaccg cacgggcggc gttctctgcc agccattgtg ggaactgacc caacaactgc
      841 acgcgcacca gcgtaccgga tggtgtccgg ctggcgtttt gtagctcagg gcaaatgcga
      901 tgaaagactt tcaccggcag cagttgatcc accacgcgga cgaactcggt gatgcacaga
      961 tcgtagtcgt taacttcggt cagcaattca cgcaccagag agtcaagcac tccctccatc
     1021 ggagccagta acacacgcat atcatcaccc gcaaaaaaat gaggcgctat gttagcgcct
     1081 cctgtgtttg cagacaaact gacttactga ttaattcggg caagattttt accctcaccc
     1141 taaccctctc ccgaaggggc gaggggaccg cccgtgcgcc tgattgggtg aagaagtgct
     1201 tagcgtggtt gcaaacagtt atcttctttc cagccgtaca accactccat gccgcgataa
     1261 aattctgcct gcgatttccc tttccacagc agattacgta cctggcgttc tacccctgcg
     1321 gcgtggtaaa cccgccccat ttcacgggtc gaccagacga tgcgcgcggt gcgcggaatt
     1381 cgtaccgact catacagcgc aaaggcttgc gccgcatcac cctcacactg cgccagcgcc
     1441 ttacccagcg ttactgcatc ttccagcgcc atacaggctc cctgcgccat atattgcgcc
     1501 accggatggg ccgaatcgcc cacaagcgtt atgcgcttcg ttccccactt cgccaccggt
     1561 tcacggtcag cggttgacca gcgtcgccat gaagtcggtt tatccaacat ctggcgcgga
     1621 cgggggtgaa tcccttcaaa gtaagagagc acttcctctt tactaccatc cttcacgccc
     1681 cactcttcct gctggcggct gtggaatgtt accaccaggt tatactgctt gccgccgcgc
     1741 aaagggtaat gcaccaagtg acaatgcggc cccgcccaca gtaccggtgc gttaatgcgc
     1801 aaatcttccg gcatatcggc acgatcgata accgcacggt agacaacatg tccggtgaca
     1861 cgcggcgcat cgcccaataa gctctggcgc accactgact tcacgccgtc acagccaatc
     1921 agaatatcgc cggtccagct attgccgcga tcgtcaaaca cggtgacatc gtcttccgtc
     1981 tcgcgaatat ccacgacgtt ggtggaggtg tgatattcca cgtccggatg cgtcagtacc
     2041 gcttcccaca ccgaggcgtg aatatcaacc cggtggatca ccgcgtaagg gccgccgaaa
     2101 tggtcacgaa aggcctgccc ggtttcaatg cacaccactt cttcggcgtt caccgcatcc
     2161 atcatggtaa tgtgatcggt aaacaccgcg cgctggcggg caacttcgcc gacgccgagg
     2221 ctgtccagcg ccgaaaacgc atttggcccc aactggatgc ccgcgccaat ctcaccgatc
     2281 tcatgcgcct tttccagcag caccactttg atccccaggc gggccagtga cagcgcagtt
     2341 gccgcaccgc cgatcccgcc tcccacaatt actgcgcgtg tcactttcgt cattatagtt
     2401 ctcctggtca ggctgaaatt ttatcttgtt gattttctgg cgctgcggca atgaacgctg
     2461 gcagctgtgt gcaggcgtcg tacaccgctt tgcagcgtgg atacccgctc aaatcgcagt
     2521 tcatgcgtag cgcgttcgcc cactgcggga caaggcagca atctgccagc gtcggcgtct
     2581 cgcccacaca aaattgcccc gactggttct ggcgcaacag ttgttccacc gcgcttaacc
     2641 cttgctggat ccagtgcgca taccaacgtt ttttttcttc ttcactgacg ttcagttctt
     2701 cggtcaggta gcgcagcacg cgcaagttat tcaatggatg aatatcacaa gcgatggcat
     2761 agacaatttc cagcaccttg ttgcgggccg gctcttcctg cggcaccagc ggaacttgcg
     2821 ggtagtgcct ctccagccag tcaataatcg ccagcgattg tccgagtgat tcgccctcgt
     2881 cagtcagcag cgtcggcacc agcccgaccg gattcatccg ccgataggcc agttcatttt
     2941 gctgaccaat acggatattc accccttcgg tctggtaatt aatgcctttc agcgccagcg
     3001 caatgcgtac ccggtacgac gctgaactat taaaaaaact gtacagcttc atcatgcacc
     3061 tcagacaatc ttcaccgcga taggcgttaa gccctcgacg ttgcctgtga tcacatcacc
     3121 tttcactacc gggccaaccc cttccggcgt accggtgaag atcagatcgc ccggttgcag
     3181 ctcaaagaag cctgacagat agcagatggt ttcattcacc gaccagatga gatggcgaat
     3241 atcgctgcgc tgatgatctt cgccgttaac ctgtaaccag attggcgcat tatcaatacc
     3301 gccgatatcg cgggctttgt gcagcggcgc gatgggcgct gagagatcaa acgctttacc
     3361 gatttcccac gggcggccca tctggcgcat ttccatttga cggtcacgcc gagtcatatc
     3421 aagacccgtg gcataacccc agacatattc atgggcgtgt tccagcggga tatcgctgcc
     3481 ttttttacca atcgccacca ccagttcaat ttcgtaatgg tagttatcgg tctgtgccgg
     3541 atacggcagc gccagcgttt caccagcggc aaccggaaca acggcatccg ccggtttgca
     3601 gaagaagaat ggcggttcac gatccggatc aaagcccatt tcgcgggcgt gagcggcata
     3661 gttacgtccg acgcagtaaa cgcggcgcac ggggaattgt tcatcactgc caacaaccgg
     3721 tacggtaacg ggcgcttgcg gttcgaatac gtattgagtc atttttctct cctaaattaa
     3781 taacgcgctt cgcggaacag ccccagggct tcttgtaccg gtctgtccga aaaactaaat
     3841 aaaacggaat cttgcgtggt ctggaacgac acgccatgcc aggtcggcac cacgaagata
     3901 tcttttgcac taaagctgaa ggtttcattg ccgatgatga cctgcccgct accttccacc
     3961 acatggtaga tggtgctgtc agtggtacgc gcaacgcgtg aggcaaaccc tttcggcagc
     4021 agttgcagga aagcgcccat cgacggcatc ggatagccgc cggtgaccgg gttgacgtag
     4081 cgcattttgt agccatccca ctcgtcggca tcgcccagtc gggttaaatc gtgcagcact
     4141 tcgcggctgc ggtcataacg atagttaaag atcggcgagg agttccccgt ctgatgccgc
     4201 agcggcaaca tattggcagc gtaacgcggc agataatctc cctctttacg cgttaccggt
     4261 tgttgctctt ccggataatc ttcggcaaaa ccgcagccca gaatattcac taatggcaga
     4321 tccagcccat cgagccagat aaccggttcg tcgccagggt taccgtgatc gtgccagcgc
     4381 cactgcgggg tcaggataaa atcgccttca ttcattggcg tgcgttcgcc atcaacggcg
     4441 gtaaatgccc ctttgccttc aacaataaaa cgcagcgccg actgattatg acgatgactc
     4501 ggtgccactt cgcccggcat gatcagttgc aaaccggcat ataaagtcgc ggtaatggaa
     4561 gactgcccgc gcagcgccgg gttttccagc accagcacgc ggcgcacggc ctctttcgca
     4621 ccaatcaggc cgccgctctc cagtagcagc gggcggatct cctgataatt ccagtacgct
     4681 ggcgcacagt tagcgttggg cgttttcggc accagatggt gcagtgactc ccacagcggg
     4741 gtcaggtttt gcccggaaat atgctgataa aattgctcgc ggctattttg attgttatca
     4801 gtcattgaga tgctccttat tcgttcacaa cgcctgcgcg aggcagtgag tcagggacag
     4861 aggtcgactg gcgaaccacg gtaatcagca gggttaacat caccgcactg atcgccgcag
     4921 gcacagcgat aatgaaaaac agggtgtcga aagagaaatt catcgccatc atcacgccgc
     4981 cagacagcga accaacaatc gcgccgcagc ggccgatagc atttgaccag ctcacgcccg
     5041 tggctcgact ttgtgttgga taaagtgttg cggtcagcgc attcagcccc acctgcgatc
     5101 cgctaatgcc aatgccagtg ccgaaaatcg ccagcgccat caaccataat ccatcctggc
     5161 ttaagccaat catcacgatg catatagcgc ctatggcata gctcagcgtc agcacgcgga
     5221 aaggattgaa cttatccatc agcacaccca gcgccagcgc gcctaacgta ccaccaatct
     5281 ggaacgcggc ggtgacccag gacgcgtgtt gcaggtcgat gccgcgatgg ttcagtagcg
     5341 tcggcatcca gcttgagagc aggtaaataa tcagcaggct cataaagaac actacccaca
     5401 gcatcaacgt gatgggtaac tgacgtccca taaacagttg acgaatgctg ccttttgtaa
     5461 cggaggctga ttcaatgaga taaaagtggg tatcgacata acgctcgcgg gtgatggcgc
     5521 tgacggtttt cgcaatcacc gcctgcggta gctggcggcg cacctgccag cgtggcgatt
     5581 ccggcagaac taccagcagt gcgacaaaca gcatcagcgg taacacgccc cccaacacca
     5641 gaatgccgtg ccagccaatc accggcacca gctgcgcact gacaatcccg ccaaacgccg
     5701 aacccagcgt aaagccgcaa aacatcagcg tcaccagcgc gccgcggcgg cgggcaggca
     5761 gatactccga ggtcatggtg atggtgttcg gcatcgcgcc gcccagaccc aggccggtga
     5821 gaaagcgcag gaagaccagc gtttgcagat ccggtgagaa agccgaggcg aggcttaacg
     5881 cgccaaacaa aaagacgcac agctcaatca cccgcttgcg accaaagcga tcggacaacg
     5941 ggccacaaag cagtgcgcct gcggttaatc ctagcagacc cgcgccaaaa agcggggcga
     6001 gatcgcctgc ggtgagttgc cagtgagtgc gaatatcagg ggcgataaat ccgatagccg
     6061 cggtgtcaaa accatcgagc attaccacca gaaaacagca gatgataacg cgccactgca
     6121 tcttgccgac cggagcagca tcgataagtg cttgtaaatc acgtcgttga gtcatagtaa
     6181 atgcctcagt gcaggtaagg tagatgtttc ttttgttttg tatatgttgc gatattgtga
     6241 ttaaagagat gagatgtaca atggcatttc gggcagagag ataaccttga ggttatggca
     6301 aatggcgaac tgggcgcaaa aattaaaact acatcacctt gaaatgctgg tggcgctggg
     6361 tgaacagggc aatctaaccc atgtcgcacg aatgatgaat atcacccaac ctgcgctttc
     6421 gaaatggttg tcacaactcg aagatgagat tggaatcaca ctttttgagc ggcacagcaa
     6481 aggattacgt ccgactgaag gcggcaaatt gttattgcaa catgcccaac ggttaatcaa
     6541 tgacatggag cgatcgcaac atgaactggc ccgttttaaa caaggtggac tggtcggcag
     6601 tctgaaaatt ggctgttctc ccgtcgccac tgactgcgta tcccaggcga ttttgcattt
     6661 gcttacgaag atgccaactc tacacctgaa tatcgaagag aaggtgatga ccccattgct
     6721 gcaagatcta ttggccggtt ctgtcgatgt ggtcgttggg cgaattggcg gtcgcgcatt
     6781 gcagctgcca ctgaattatc aggtgctgta caccgagcca gtatgctttg tcgcccgccc
     6841 tgagcaccca ctggcgaaat acgccaccct cagctggaat gatcttgcca actggcgctg
     6901 gatcgtctgg ccgacgggta cccccattcg cattagtatt gataatgcgc tggtagacca
     6961 cggcgtaatg ctgccagaaa acacgattga gtcggcgtcg atgaacgtca gtaccaacct
     7021 gttacaaagc agcgatatga tctctatttt gtcattacgc ctggcgcaac gttatgccag
     7081 ccagggacaa ctggtgatac tcaacctgcc aaagatagaa cagaaaggta gcgtcggtgt
     7141 gttctggcgc aataacgaaa cgccttctta tgctttaagt cagtttctgc actttttaca
     7201 gcagaattag tgtgctgaac ggggcgctcg cccgaggtta cctccctaaa attcaactat
     7261 gctgagaatt cttaatgtga tcggtagcac gttttacgaa ttaattgtat gatgaatcca
     7321 tctcatctgg ggtgttgatt atgagtaaga cactgaacat tatctggcaa tatttacgcg
     7381 ctttcgtcct gatttatgcc tgcctgtatg caggcatttt cattgcttcc ctgctaccgg
     7441 taaccattcc gggcagcatc atcgggatgc tgatcctgtt tgtcctgctg gccttgcaaa
     7501 ttcttccggc gaaatgggtc aatccggggt gctacgtact gattcgctat atggcgctat
     7561 tgtttgtgcc gattggcgta ggcgtcatgc aatattttga tttgctccgc gcacagtttg
     7621 gcccggtagt ggtttcctgt gcaatcagta cgctggtggt ttttctggtg gtgagctgga
     7681 gttcgcaact ggtacacggt gaacgtaaag tcgtaggtca gaaaggatca gaagaatgat
     7741 ggcgaatatc tggtggtcat taccgctgac gttgattgtc ttttttgccg cccgcaaact
     7801 ggcggcacgg tataagtttc ccctgcttaa cccgttgctg gtagcaatgg tggtgatcat
     7861 tcctttttta atgctgaccg gcatctctta cgacagttac tttaaaggta gcgaagtgct
     7921 aaacgatctg ctgcaaccgg cggtcgtcgc gctggcctat cctttatatg agcagctaca
     7981 ccagatccgc gcgcgctgga aatcgatcat caccatctgt tttatcggca gcgtggttgc
     8041 gatggtgacg gggacttccg tggcattgtt gatgggcgct tcaccggaaa tcgccgcgtc
     8101 aatcctgccg aaatcagtca ccacgcctat tgcaatggcg gttggcggca gtattggcgg
     8161 tattccggca atcagcgcag tttgcgtgat tttcgtcggc atcctcggcg cggtatttgg
     8221 ccatacattg cttaatgcga tgcgtattcg taccaaagct gcgcgcggtc tggcaatggg
     8281 gactgcctcg cacgccctcg gtacggcgcg ctgcgccgag ctggattatc aggaaggtgc
     8341 atttagttcg ctagcgctgg tgttatgcgg gataattact tcgctgatcg caccgatcct
     8401 tttcccgatt attctggcag taatgggcta aaatttgcga tgcgtcgcgc atttttgatg
     8461 taagtttcac gcgttgcata attaatgaga ttcagatcac atataaagcc ataacgggtt
     8521 cgtaaactgt tatcccatta catgattatg aggcaacgcc atgcatccac gttttcaaac
     8581 cgcttttgcc caacttgcgg ataacttgca atctgcactg gaacctattc tggcagacaa
     8641 gtacttcccc gctttgttga ccggggagca agtctcatcg ctgaagagcg caacggggct
     8701 ggacgaagac gcgctggcat tcgcactact tccgctggcg gcggcctgtg cgcgtacgcc
     8761 attgtcgaat tttaatgttg gcgcaattgc gcgcggtgtg agcggaacct ggtattttgg
     8821 tgccaatatg gaatttattg gtgcgacaat gcagcaaacc gttcatgccg aacaaagcgc
     8881 gatcagccac gcctggttga gtggtgaaaa agcgcttgca gccatcaccg ttaactacac
     8941 gccttgtggt cactgccgtc agtttatgaa tgaactgaac agcggtctgg atctgcgtat
     9001 tcatctgccg ggccgcgagg cacacgcgct gcgtgactat ctggcggatg cctttgggcc
     9061 gaaagatctg gagattaaaa cgctgctgat ggacgagcag gatcacggct atgcgctgac
     9121 tggtgatgcg ctttctcagg cggcgattgc ggcggcaaac cgttcgcaca tgccttacag
     9181 taagtcgcca agcggtgtcg cgctggaatg taaagacggt cgtattttca gcggcagcta
     9241 cgctgaaaac gccgcattca acccgactct gccaccgttg cagggagcgt taattctatt
     9301 gaatctcaag ggttatgatt acccggatat ccagcgcgca gttctggcag agaaagccga
     9361 tgcgccgttg attcagtggg atgccacctc cgcaacactg aaagctctcg gctgtcacaa
     9421 tatcgaccgc gtgcttctcg cttaagcctg gtgccggatg cggcgtgaac gccttgtccg
     9481 gcttgccagc cctctcctgg tgtcgaaaat cccggcaaac agtttgtcgt ttcttgcgca
     9541 aaaccagcag gtaaagtagc ctgatggaaa ttttccttag atcgagtctc ctgcatgtta
     9601 aagcgcgtgt tcctcagcct gttagtcctg atcggcttgc tgctgttgac tgtgctcggc
     9661 ctcgatcgct ggatgagctg gaaaaccgcg ccttatatct acgacgaatt gcaggatctc
     9721 ccctaccgcc aggtcggtgt ggtgctcgga acagcaaaat attatcgtac tggcgtaatt
     9781 aatcagtatt atcgctaccg cattcaagga gcgattaatg cctataacag cggtaaggta
     9841 aattatctat tactgagcgg cgataacgca ttgcaaagtt ataatgagcc gatgaccatg
     9901 cgcaaagatt taatcgctgc tggtgtcgac ccatcagata ttgttctcga ttacgcaggc
     9961 tttcgtacgc tggattccat cgtgcgtaca cgcaaagttt tcgatactaa tgatttcatt
    10021 attatcaccc aacgtttcca ctgtgagcgg gcattattta ttgcgctgca tatggggatt
    10081 caggctcagt gttatgccgt accgtcaccg aaagatatgc tgtcagtacg tattcgtgaa
    10141 tttgccgccc gtttcggtgc gctggctgac ctttatattt ttaaacgtga accgcgtttt
    10201 ttagggccgc tggtccctat tccggctatg caccaggtgc cggaagatgc gcaggggtat
    10261 cccgccgtca cacccgaaca gttacttgaa ttacaaaaga aacaaggaaa gtagttatgg
    10321 atgtacagca gttttttgtc gttgccgttt ttttccttat cccgatattt tgtttccgcg
    10381 aagcatggaa aggctggcgc gcaggcgcga ttgataaacg ggttaaaaat gcaccggaac
    10441 cggtgtatgt ctggcgagca aaaaatcccg gactcttttt cgcttatatg gtggcatata
    10501 tcggcttcgg aattttatct atcggcatga ttgtttatct tattttctat cgttaatatt
    10561 ttctcttttc tgttaattat tgagaataat tattacttca ctcaataagc tgcggatata
    10621 acattctgaa ccgcagctat ttgtgaatcc tttcacagtt taaatttccc cacgcgcata
    10681 gccttaatat cagcacgcaa tatttattta gcgttcgcct taattactca tagcattaag
    10741 gaagatcaca tgccgcaaca aaattatctg gatgaactca ctccggcttt tacgccgtta
    10801 ctggcgatta aagaagcctc tcgctgttta ttatgtcacg acgctccctg tagtcaggct
    10861 tgcccggcac agaccgatcc ggggaaattt attcgctcaa tctactttcg taattttaaa
    10921 ggcgctgccg agacaattcg cgaaaataat gccctcggtg ccgtttgtgc cagagtgtgc
    10981 ccgacggaga aattatgcca aagcggttgt acccgtgccg gtgttgatgc tcccattgat
    11041 atcggccgct tacagcgttt tgttactgat tttgaacaac aaaccgggat ggaaatttat
    11101 cagcccggta ctaaaacgct cggcaaagtc gcgattattg gcgcaggtcc tgccggattg
    11161 caggccagtg tgacgctaac aaaccagggt tatgacgtca cgatttatga gaaagaagcg
    11221 caccccggtg gctggttgcg taacggtatt ccgcaatttc ggttaccgca atcagtgctg
    11281 gatgcagaaa tcgcccgtat tgaaaaaatg ggcgtgacca ttaagtgcaa taacgaaatc
    11341 ggtaaaacac tcaccctgga gcaactgaaa gcggaaaacc gcgcggtact ggtcaccgtg
    11401 gggttatcaa gcggttccgg gctaccgctg tttgagcata gcgacgttga gattgccgtc
    11461 gacttcctgc tacgtgcacg acaggcgcaa ggcgatatca gcattccaca aagcgcatta
    11521 attatcggcg gcggtgatgt cgcgatggac gtagccagca cgctgaaagt tctcggctgt
    11581 caggcggtaa cttgcgtagc gcgtgaagag ttagatgagt ttccggcaag cgaaaaagag
    11641 tttgccagcg cccgggagct gggcgtttcg atcatcgatg gattcacgcc agtagccgtg
    11701 gaaggcaata aagtcacgtt taagcatgta cggttatcgg gcgaactgac gatggcggca
    11761 gataaaatta ttctcgccgt cggtcagcat gccagactgg atgactttgc gaagttagag
    11821 ccgcagcgta acaccatcaa aacacaaaat tatcagaccc gcgatccgca agtctttgct
    11881 gctggcgata ttgttgaggg tgacaaaacc gtggtctatg ccgtgaaaac cgggaaagaa
    11941 gccgccgagg cgattcatca ctatttagag ggagcttgct catgttaacg aaagatcttt
    12001 cgattacttt ttgcggcgtg aagtttccca acccgttctg cctctcttct tcgccggtag
    12061 gcaactgcta tgagatgtgt gccaaagcct acgacacagg ttggggcggc gtggtgttta
    12121 aaacgatcgg cttttttatc gccaacgaag tctcgccgcg ttttgatcat ctggtgaaag
    12181 aagataccgg ttttatcggc ttcaaaaata tggaacagat tgctgaacat ccgttggaag
    12241 agaatctggc cgccctgcgt cggctgaagg aagattaccc ggacaaagta ttgatcgctt
    12301 cgatcatggg ggaaaatgag cagcaatggg aggagctggc gcgcctggtg caagaagctg
    12361 gcgcggatat gatcgagtgt aacttctcct gtccgcaaat gacttctcat gcgatgggta
    12421 gcgatgtcgg gcaaagcccg gagctggtag aaaaatattg tcgggcagtg aaacggggtt
    12481 ccacgctgcc aatgctggcg aagatgacgc cgaatatcgg tgatatgtgc gaagtggcgc
    12541 tggcggcgaa gcgcggcggc gcagatggca ttgcggcgat taacaccgtt aaatccatca
    12601 ccaatatcga tcttaatcag aaaatcggta tgccgatcgt taacggaaaa tcgagtattt
    12661 ccggatattc cggtaaagcg gtaaaaccga tcgccctgcg cttcattcag caaatgcgca
    12721 cccatccaga actgcgcgat ttcccaatca gcggtatcgg cggcattgaa acctgggagg
    12781 atgcggcgga gtttttattg ctcggcgcag caacgttaca ggtcaccacc ggcattatgc
    12841 agtacggcta tcgcatcgta gaagatatgg cgagcgggtt atcgcattat ctcgccgatc
    12901 agggatttga ttcgttgcag gagatggtag gtctggcgaa taacaatatt gtcccggcgg
    12961 aagatttaga ccgcagttat attgtctatc cccgtatcaa tcttgataaa tgtgttggct
    13021 gtggacgctg ttatatttcc tgttacgacg gcggtcacca ggcgatggaa tggagcgaga
    13081 aaacccgcac accgcattgt aataccgaga aatgtgtggg ttgtctgctt tgtggtcacg
    13141 tctgcccggt gggttgtatt gagctcgggg aagtgaagtt taagaaaggc gagaaagaac
    13201 acccggtaac gttgtaaaac cagagacgca tccggcattt ggttcctgtc gccggatgcg
    13261 gcttgaacgc cttatccggc ctacaaaacc atacaaatca gtatgttgag ctcattgcag
    13321 gccggataaa acgcgccagc gttgcatccg gcatttggct cctgtcgccg gatgcggcgt
    13381 gaacgcctta tccggcctac aaaaccacac agatcatgat gttatgtgcg tttatgaccg
    13441 aatgcggacc acaatcattt cacttcttac gcgcgtactt cagtgaatcc agcgctacgg
    13501 cgaagataat aatcgcccct ttgatgatgt actgccagta tgggtttacg ccgatgtacg
    13561 tcaggccata gttgatgacg gtaaaaataa ttaccccggt caccacgcca atcaccgtcc
    13621 ccacaccgcc gctgaacgat acaccgccta ccacgcacgc cgcgatagca tccagttcat
    13681 acataaagcc gaggttgttg gtggcagagc cgatacgtcc ggcttctaac atcccgccaa
    13741 aggcatagaa cacgccagac aacgcgtaga tcatcagcag gttcaggccg acgttgacac
    13801 cagatacttt tgccgcttcc gggttaccgc caatggcaaa aatgttctta ccgaagcggg
    13861 ttttgttcca caacacccag acgaacgcca ccgcaatcaa cgcgtagaag gtgatgtaag
    13921 agagacggaa actccccagc gcgacaaagc cctgagcaaa ggtagagaag ccactgtcaa
    13981 aaccagaaat tggcgacgcc ccgacaaagt cgtaataaag cgagttgatg ccatagacga
    14041 tgatcatcgt gccgagcgtg gtaatgaacg gcgtcacgtt gagataagcg ataatcagac
    14101 cgttgatcaa accgatcacc gcaccaatgg cacagacaat cagaataacc agcgcaatcg
    14161 gcatcgtcgc catttccggg aacactttgt tggcgttatc catggactgc aataatgtcg
    14221 ctgccaccac tgccgccagc cctacttgac gccccgcaga aaggtcggtc ccctgggtga
    14281 caattaaccc tgccacaccg agcgcgataa taatacgcac cgatgactgg gtgagaatat
    14341 tacttaagtt caacagactt aaaaatgttg gggcctggaa aataataatc gcaagcaaaa
    14401 ctaaaagaac gacgtaaata ccgccctctt tcaggtaagt aagaaaactt ttcttattta
    14461 acgcactcat gggaagcccc taatcttaaa ggtgcaaaga cgcaagacgt agaatttcgt
    14521 tttgcgttgt tgtttttgta tcgacaattc cggaaacgag gccattgctc ataaccagaa
    14581 tacggtctgt tatccctaac aactctggca tttcagagga gataataata atccccttgc
    14641 ctttcttcgc cagttcggca attaactgat aaatctcaaa cttcgcccca acatcaatac
    14701 cgcgcgtcgg ttcatcgagc attaatattt ctggttgcgt taacagccag cgcccaataa
    14761 tcaccttttg ctgattacca cctgagagcg aaccaatttg cgtccgatgc cccggtgttt
    14821 ttacccgcat cgaatcaatc acccactggg tatcgctttt catccgcgag ttatccagta
    14881 aaccaacttt atttttgtag ttgcgaatat tggaaattaa cgagttaaaa ccaatatcca
    14941 gataggcata aattcccgtt gagcggcgct cctcagttac cagtgcaaat ccgtggttta
    15001 tggcttcgtt ggcattatgg ttattgatct ttttgccgtg caacgtaatg gtgccagccg
    15061 atttctcgcg aataccaaat aacgtctcaa caatatcggt acgtttcgcc cccaccagac
    15121 cggcaatacc gaggatctcc cctttatgca gatcaaacga gacatcgcga atcgacggct
    15181 ggcgcagtga cgtcaggtta cgtacctcga ggatgacttc gcccggcttg ttttctttgt
    15241 caggaaaacg ctggttaaga gaacgcccaa ccatcatggc gataatcttg tccatcgtca
    15301 gccctgccag cggttcggtg gcgatccact gaccgtcgcg caatacggta acttcatcac
    15361 ataactggaa gatttcctcc attttatgcg agatataaac aataccgcag ccgcgctctt
    15421 ttaatttacg aataatagtg aacagatgat tgacctcttt ttcggttaac gaagaagtcg
    15481 gttcatccat aatcacaatt ttcgcgttat aggaaaacgc tttggcgatt tcgatcatct
    15541 gcatttgcga aacagataat gtgccgacgc gcgcacgcgg atcgatatca atatccagtt
    15601 catcaaaaat cgctttggtt tcgcggtaca ttttgtcctg atcgacaaac atgcctttgg
    15661 tggggtatcg cccaagccac atgttgtcca tcaccgaacg ttgtaatacc aggtttaact
    15721 cctggtgtac catcgaaata ccattttcca gtgcttcttt ggccgaatgg aaatcgatct
    15781 ctttaccctg gaataaaatg gtgccggagt cttttttata aataccaaac aggcatttta
    15841 ataatgttga ttttcctgcg ccgttttccc ccattaatgc atggatagaa tgtggccgga
    15901 cttttaaatt aacgttatca agtgccttaa caccaggaaa agacttgttg ataccgctca
    15961 tttccaacaa gtattccccg gaagacggag tcgttgagct gaccatataa ttttaccttg
    16021 ttggccatac aataagggcg cagtaataga ctgcgcccaa tcagtcttat ttcttgctga
    16081 attcagccag gttgtcttta tctacgccaa cataaggtac gcggaccact ttgttgtcga
    16141 ttttccagtt ggtgccatca gccgcacctt taccatcggc caggtttttc gccagatcaa
    16201 aggtcgcttt cgcctggttg ttagcatcgt tcagtacggt gcccgccagt gcaccggatt
    16261 tcaccagcgc cagcgcttct ggcagagcat cgacgccaaa caccggaatg ctggacttgt
    16321 tgtgtgcttt cagtgcttct accgcgccca ttgccatcgc atcgttgttg gcgataacca
    16381 cttcgatttt gttggcgttc gggccagaca gccaggcgtc catcttatct ttcgcctgag
    16441 cggtatccca catagcggta tctaactgta actgttcagt tttaatgcct ttgtcgttca
    16501 gctctttaat cacgtaagtg gtacgtgctt ctgcatccgg atggcccggt tcacctttca
    16561 gcagtacgaa ttgaatctga ccgtctttat tcagatccca accctgattc gccgcccagt
    16621 gtttagcaat caaatcgcct tgaataatgc cggactcttt ggagtcagtg ccaacgtagt
    16681 aggctttgtc gtagctatcc agcgccttac gagacggttc tttgttaaag aataccaccg
    16741 gcacattttg cccacgcgct ttctcaatca ccgtacccgc agctgccggg tcaaccaggt
    16801 tgattgccag tgccttcacc cctttcgcca gcaatacgtc gatctgatcg ttctgcttgg
    16861 actggtcatt ctgtgaatca ttcatcagca gctgaacatc tggcgcggct ttcgcatctt
    16921 gctcaatagc cttgcgcact acagacataa agttatcgtc gtacttatag attgttacac
    16981 caatgcgagt atcagcagcg tgtgcagtgg caccgaataa catgctggcc atcacagcag
    17041 acagggttaa caccttctta ttcatggtat ctccggtttt tcttatgcag ggtagtgctt
    17101 gagataaatg ctcggcgggg cagtagagtt aatgaagtgt tactgaacgc cgaagctcac
    17161 tttttaaaat tcgttcttcc atgcccggta acgctccaga aaacggcttt aattgttgtt
    17221 atgacgcatt acctcggtga aagtgattat tcactgttac atacgggtta caacgttaaa
    17281 acggtgcaat catagctatc acattgttaa gatactgtga aatcactcac agattgaaag
    17341 cggttacatc gcctgatttg ttgagttagt gatcgccgcc gcattctggc gtgttgcgac
    17401 agaatggcga cgcactaacg tcggcataaa acagtggctg gcacgaggat caatattgcc
    17461 tgctgccccc tgcaaggcca gttcggtggc taatttcgcc attgaagcaa tgggataacg
    17521 cacggtcgtt aattgcgggt cggtgtaacg ggcgatggga atatcatcga aaccgatgat
    17581 tgagagatgt aacggaatcg caatgccatt atcttttaat gctgtcagtg cgccagcagc
    17641 catattgtcg ttataagcaa atacagcggt aagttgtaga ttgcgcccca gcagttcaac
    17701 catcgccgcc tcaccgcccg gcatgtccgg cgtaccagtg ccaatccagc tttccggcgg
    17761 aataatatcc tgctctttca acgcactcat ccagcctgct ttacgcatgg cgtcatcttc
    17821 aatgccgtgg ctggaagaaa gataaccaat acgttgatga ccgttattca gcaacatgcg
    17881 cgtcgccatt cgggcaccgc tgagattatc caggcaaacg caacgatggg tgtaccccgg
    17941 cacaacgcgg ttgattaaca ccataccggg aatgttatcc ataaattgcg ccagttcatc
    18001 gtcactcaat gcttttgagt gaacaatcaa cgcattacaa cgctggcgaa ttaacacctc
    18061 aatggcgtga cgctcttttt ccgcttcatg atagctattg ccgattagca cgtatttctg
    18121 atgctgctga gcgaccagat ccaccgcttt taccagcgcg ccgaaaaacg catcagaaac
    18181 gtccatcacc accacgccaa tggtgtcgct aacctgagtt gccagcgcct gggcattggc
    18241 gtttggccga taatccagct cactcacggc tttcattact gcttcacgcg tgtcggcact
    18301 gaccagcgtg ctgttattga gcacccggga aaccgttgcc acagagacgc cagcctgacg
    18361 cgctacatca cgaatggtga tcatattcac tacctgttat gagattgcag taaatgactg
    18421 cttgctgccg gctattttgt cagcactgaa tacaggactt cgtgaatcga gtcacagcaa
    18481 tggaaacggt tacagccgtt ttgttaacga atgtgatcca gatcgttatc ttgatgtttt
    18541 agatattgcc ggtccggcag cacgcaaagt taactgacgc cagagccatt ccaccggccc
    18601 ctggcggaag taacgcagcc agataacaga gaagagaata ttcgccagcc ataccggaat
    18661 aacaaacgcc agcaattcca gacggtcaaa ctgcataaac agaccgaggt ggtaaaaaag
    18721 cgtggtacaa atcagcgttt gcaatagata gttggtcagc gccatccgtc cgacgcaggc
    18781 gatcgccagc accagcttaa agcggctcaa ttgcggccag aagccataaa acagcgacgc
    18841 atagccaatc gcctgaaacg gcgcactcag ttcccgcggc atttgaagta agaaggcaca
    18901 ccagcgatac gcccagtcca gctgccattg cagggcgatg gcaggaaggt taatgatcac
    18961 cccaatcgcc actagcacaa aaccagtacg acgatagtga cgtaagctga actgcccttt
    19021 cagccagcca ctgcgcatca atgcggcacc aatgagcatc atcccagcca gttgccagcc
    19081 atactgtgcg cccagtgcca ataaactgtt cccaacacca tcggcacgat tactgatcgc
    19141 ttcaacgccg ccgtgaagct tccagtattt ttcatataaa atagccgatg catccggcgt
    19201 ccaggcgcgg ctagtctggc tatcggaaat caaccccaat aacagcaaaa cgccaagccc
    19261 caccagataa agcatgacgc cggtattaaa aaggcttttt accgatggcg catcgcgcac
    19321 cagccgccag cagattaagc ccaccagccc gtaagccagc agaatatcgc cgtcccagaa
    19381 caataaaccg tgaataaagc ccagcaagac taacagcgtt aaccgcgact ggatccagcg
    19441 tctgccacgg ggcagcaaca tttgcaggcc agcaccaaac agcagcgcaa aaagcgtgag
    19501 gaatttcacc tggccgatga gatcgagaaa tgcccaggtc catgcatcct gcggcgtaat
    19561 agcgccgtac caggcgggat taagataagc cgcctttggt agcccaaagg cgctgatgtt
    19621 taatagcagg atccccagaa tggcgacgcc gcgaacaaag tcgagcgtga cgttgcgctc
    19681 catggttcct gcctttaaat cagttgtgat gacgcacagc gcgcagaaac tcgtggcgcg
    19741 tattctgact ggatttgaac aatccaccaa gagaggtcgt tgtcgtggca ctggttgcat
    19801 cgcggatgcc acgcgccttc acgcaataat gcaccgcgtc gatagagaca gccacgttat
    19861 tggtgcccag cagcgtttgt agcgcaatga gaatttgctg cgtcagacgt tcctgcacct
    19921 gcggacgctg ggcaaagaac tgcacaatac ggttaatttt tgacagaccg atcaccgaat
    19981 ctttcgggat ataagccacc gtcgctttgc catcgatggt aacaaaatgg tgttcacagg
    20041 tactggtcag agtgatatcg cgcacggtga ccatttcatc gaccttcatt ttgttttcaa
    20101 tgagggtgat tttcgggaag ttggcgtaat ccagaccgga gaaaatttca tcgacataca
    20161 ttttagcgat gcgatgcggc gtttccatca aactgtcatc agccaggtcg agattcagca
    20221 gctgcatgat ttcggtcata tgaccagcaa taaggctttt gcgcgtttcg ttatccattt
    20281 catgcacggg cgggcgcagc ggtgtttcca gtcctcgcgc aactaacgct tcatgaacca
    20341 gggccgcttc tttactgagt gatggcattt atgatttctc ctgcaggtgt gacgcctccg
    20401 ccctgcgtgg gggcaaagtt attaagctga tttacagcct gattattgtg cgtgaggcgg
    20461 cgcacataat ccagtattca cagcgataat tattgtaatt gccgctgcct ttcatcagca
    20521 gatgttaaaa catcgttatg caaatacgga agtgaaagtt actcacagca cattgaataa
    20581 acggtatgat gaagaaattg caaacaacac aacaaggagc cacgcatgga aatgctcgaa
    20641 gagcaccgct gttttgaagg ctggcagcaa cgctggcgac acgactccag taccttaaac
    20701 tgcccgatga cgttcagtat ctttctccca ccccctcgtg atcacacccc gccaccagtg
    20761 ctgtactggc tttccggatt aacctgcaat gacgagaact tcaccaccaa ggcgggtgcc
    20821 cagcgcgtgg cggcggaact ggggattgta ctggttatgc cagacaccag cccgcgcggc
    20881 gaaaaggttg ccaacgacga tggctacgat ttaggccagg gcgcaggctt ttatcttaat
    20941 gccacgcaac cgccgtgggc gacgcattac cggatgtatg attatctgcg tgatgaatta
    21001 ccggcgctgg ttcagtcgca atttaatgtc agcgaccgct gcgctattag cggtcattca
    21061 atgggtggtc acggtgcgct gattatggcg ctgaaaaacc cgggcaaata caccagcgtt
    21121 tcggcctttg cgccaattgt gaatccgtgc agcgtcccgt ggggaatcaa agcgtttagc
    21181 cgctatttag gtgaggacaa aaatgcatgg ctggaatggg acagttgcgc gctgatgtat
    21241 gccagtaacg cgcaggatgc gatcccgacg cttatcgatc agggtgataa cgatcagttt
    21301 cttgctgacc aactgcaacc tgcagtactt gcagaagccg cgcgccagaa agcatggccg
    21361 atgacgctgc gtattcagcc gggatatgat cacagttact acttcatcgc ctcttttata
    21421 gaggatcacc tgcgcttcca tgcgcagtat ttactgaagt gaaagtccgc ccggttcgcc
    21481 gggcatcttc tcatcagaag cgataatcca ctgccataaa gtaacgacgt ccgtcttcgt
    21541 tatagctgta gtcgtcacga ctgagatcct tgtcgccaag gttcagcacg cctgcgcgca
    21601 gtttgacgtc tttagtcacc tgccaggccg cgccggtatt ccagatggta taaccgcccg
    21661 gtgttttagc cgtcgcgctg tcggctcgtt tctgcccggt atagtgacca gaaacataga
    21721 atgaccagtc ttccagcgcc agcggtttcc agtccagcgt accgttagcg gtatggaacg
    21781 gcagatcgga tagcggtttg ttttcgccgt tgctgacatc acgaccatcg ttgtaggtgt
    21841 agttgagtga cagtttccat tcatcgttga acggaatttt cagctcggtt tccacgccct
    21901 gaatacgtgc tttgttaacg ttgtagtagc taaataccgg cacaccgttc gctgtaaaac
    21961 caacaaagtt ttggtagccc ggtgcagcat tgacgtcaga cgtacggctg atgctgatac
    22021 gatctttcac atcgttacgg aaaacggtaa cgctggattc aaccccttcc agccagcctt
    22081 cttcacccat gtagtaaagc cccagctccc aactttcgct ggtttctggt ttcagatccg
    22141 ggctacccac aatcttacat gcgccacggc aggaattgct cgtccagtca gggctaagtt
    22201 gcaacagaga aggtgcttta aatgccgtcg cccagccccc tttcaccgtt acggtgtcgg
    22261 tggcgttata aaccaggtag gcacgcggac tccagtgttc accgtaggtt tcgtgatcgt
    22321 ccatacgcac gccggtcgtc agcgccagcg gctcgaagat ccgccattca tcttccacaa
    22381 acagcgcgta ctggctagca gacgttttgg agctggttcc cccggtcagg ttcaccgcat
    22441 cgctaagttt gtcgtgacgc cattcaccgc caaccgtgag aaactgatta atcgccgtca
    22501 gcggcaacgt gtatttgccg tcgaccgtat tgctttcgga agttatcggg ctgctgttgc
    22561 cagggttttt gttctcgact ttctcaccgt agtatttcag ttcgctggtg ccgtaatccc
    22621 aacgcccatt atggctgacg gagtagttct ggcgttccag gcggtttttg tccagcgagt
    22681 cggaatcacg atcctgacgg tcgaaaccgt atccggcagt aaaatcgtga ttttgattcg
    22741 gtgtccaggc aaattcgaca ttgccgttgc ggctggagaa tccttcaata cgcggcgttt
    22801 ctccggtatc ggtggtcgtt gagttttgcg ggtcatcctt ttcacgtttt gccaggctgc
    22861 cgtaagcttt cattcccagc acaccatcaa ttaatggtcc actggtaaag aactgaccgt
    22921 tataggtatc accgcgatcg cgatgttcct gaatggtggt atcgacggta acggtacccg
    22981 accatttctg accgattttt ttggtgatga tattcactac accgccgagc gcatcggaac
    23041 cgtacagcga cgacatcggg ccacggacca cttcaatacg ttcgatggaa tcgaccggga
    23101 tccagttcag atcgaaatca ttgtggcgga agacggcatt gcgggagtta acgcgtttac
    23161 cgtcgacaag aatcagggtg tagctgctgt ccagaccacg aatactaacg cccttacggt
    23221 tatccccttc gttcgtcagt tgtacgccag gcacttcttt gaggacatcc ttcagattct
    23281 gtaccggttt tcgctgcagg tcttcctggg taatgacgct gatactggcg ggtgcatctt
    23341 taaggttttg ttcaacggaa gatgcagtga caaccatcgt ttcgccatcg tcatcgaccg
    23401 ctaacacagg ccatgcacaa gaaatagcgg acaaacacag cccgacccgt acgaaagggt
    23461 tcaacctaaa cattccatat ctccatgagg taactacgaa aataaaatgg gttatcgctc
    23521 acatcttctt cacgtcccct tgcgtacggc agcatcgcgg tggacttatt tgtatgcagg
    23581 tgatcatccc agaaagccgg accagcctct gatttgtcag ccttttttga ttgcggcgta
    23641 acgataatgc aaacgataac aattatcaat ccaaatgtta aattttatat ccgcatgatt
    23701 tgtcagggaa atggaattga aaaagccctc tcggttgaga gggcttagca aggaagggag
    23761 aaacttattt cttatcgttc tgcgggaact tcatttcgct gtagcgtacg aagtgggttc
    23821 ctttaatcag cttgtagccg aaccaaataa tcaggaacag cgggatacca atatacgttg
    23881 ccgctacgcc gccccagtca atggtatctt tcaggaacgc ttcgtagttc tggcccaaag
    23941 tgataatcag acacagaatg aatgcgaaga tcggccccaa tgggaagaaa cctgaacggt
    24001 acggcagatc gttaatgtcg tgtccctgca atacgtaacc gcgacggaag cgatagtggc
    24061 taatggcaat ccccagccag gcgataaaac cggtcatccc ggaggtattc agcagccaca
    24121 ggtataccgt ctggttgcca aacatggagg tcaggaagca cagaccggca atcaccgtcg
    24181 tcgcatacag cgcattacgc ggcacgccac cacgcgacag tttagcgaaa atgcgcggcg
    24241 ctttaccgtc acacgccagg gtgtacagca tacgagtaga cgcatacata ccggagttac
    24301 ccgctgacag caccgccgtc agaataactg cgttcatcac cgccgcagca gagagcagac
    24361 ccgcgtgctg gaacaccagg gtgaacggac taacgctgat gtctttaaca tcgttacgca
    24421 gcaggctcgg atcggtgtac ggaataatca ggctgataat caggatcgcg aacacataga
    24481 acaacaggat tcgccagaac acctgacgta ccgcgcgtgg aatgtttttc cccggatctt
    24541 cggactcgcc tgcagcaata ccgatcagct cggttccctg gaaagagaag ccgacaatca
    24601 tagctacgcc gatcatcgcc gcaaaaccac cagcaaacgg cgcttcgccg attgtccagt
    24661 tgctccagcc cgcaggctgc gcgcctttga agataccgat aatcatcagc acgccaacga
    24721 tgataaagac aataactgtc gtgactttga tcagtgagaa ccagtattcc gcttcaccaa
    24781 agccgcgaac agagatgtag ttcagcagga agataacgcc gaggaacaac gcactccaga
    24841 tccagcccgg tgtatccggg aaccaccagc tcatgaccag ctgagctgca accaggtcaa
    24901 cggcaatagt caccgcccag ttgtaccagt agttccagcc cagcgcgaag ccaaagccct
    24961 cttcaacata gttctgaccg taagtggcaa acgaaccgga aaccggcata tatgcagcca
    25021 gttcaccgag actggtcatc aggaagtaaa ccatcaggcc aatcaacata tacgagagca
    25081 atgccccgcc cggacctgcc tgagaaatcg ttgcgccaga ggcaacaaaa agacctgtac
    25141 cgatggaacc gccaatggca atcatcgtca ggtgacgcgc ctttaattca cggcgtaagc
    25201 ccggcgcttc tgtggtttta gtttcggaaa ccatacaaaa atgctatcca tctaaaaaat
    25261 gaggcgcgat tgtagcagac gattggcaat ccttccggca gaaatacacg gttataagac
    25321 accttcatga tcgcccaggg attataagta aagcagccaa tctcttttct ggcgaatgca
    25381 tgctgaaggc gctgcgtcac gaaatcgaca cagcaccagc atgttcttgt acagcaactt
    25441 aacgcggcac atttgcggga tcgcaatagt ccagaaagcg ccgtagcgcg ttggaaaggt
    25501 gtttttgccg atgatgtata cgccacaacg tacgcatcag gcgcggcaga gggaccgcaa
    25561 cttcacttaa tgtgcctgcc tgcaattgat cttcaatcac acgtcgcgac aggcaactaa
    25621 ttcccaaccc atgacgcacc gcatgtttga ttgcctcgga gttacctaat tccatcgcca
    25681 tctcaaactt cggtaaatgt gacagcaaca gataatcgac aatctcccgc gtgccggaac
    25741 cgcgttcacg caggatccac ggcgcagcgg ccagctgttc taaggtgacc ggaccacggg
    25801 ccaacggcga agtcggcgcg gcgaaaacca ccagctcgtc ttccagccac ggttcagaaa
    25861 tgatttcagt gctgtggcac ggtccttcaa taaagccaat atcaacgcgg aaatccagca
    25921 ccgcttgcat cacgtcctgg ctattcccaa cgctaagttc aatcggcaac tgcggataat
    25981 catggcgata acgggcgata actgcaggca gaatgtagtt accgatggta ctactggcat
    26041 agatacgaat cgcgccgttg tcttcgcgaa acagttgttc gatttcaacc gcctgttcaa
    26101 gcaatgccaa cgcacgcgga tagagcagcc gcccgtgttc attaacaacc agtcttttcc
    26161 ccacgcgatc aaacagttgc acgccaagct gcccttccag gtcggtcaag gctgcgctca
    26221 ctgctgattg cgacaacgcc agcatcaccg acgcttgggt ggttgatcca cttttcaata
    26281 cttctgcaaa aacttccaac tgccggaggg tgatgtgcat agtcgcttac cacttataaa
    26341 gattaattat aaatatataa tcaattttat ttttaaacca gttagtcgta accttatgcc
    26401 cggttaaagg agagggttat gacgaatatc accttacaga aacaacatcg tacactgtgg
    26461 cattttattc cggggttagc cctgagtgca gttatcaccg gggtcgccct gtggggtggt
    26521 tccattcccg cggttgcggg tgccgggttt agtgccctca ccctcgcaat cttgttgggg
    26581 atggttttag gcaacaccat ctatccgcac atctggaaaa gctgtgacgg tggtgtgctg
    26641 tttgccaagc aatatttatt acgtctgggt atcattcttt atggcttccg tctgacgttc
    26701 tcgcaaattg ccgatgtcgg tatcagtggg atcatcattg acgtgctgac gctgtccagt
    26761 accttcctgc tggcttgctt cctggggcag aaagtgtttg gtctggataa gcacaccagc
    26821 tggttgatcg gtgccggtag cagtatctgt ggtgctgccg cggtactggc gactgagccg
    26881 gtagtgaaag cggaagccag taaagtaacc gtggctgttg caaccgttgt tatcttcggg
    26941 accgtcgcga ttttcctcta cccggcgata tatccgctga tgtctcaatg gtttagtccg
    27001 gaaactttcg gtatctacat tggttctact gtgcacgaag tggcgcaggt ggtggcggca
    27061 ggtcatgcca tcagcccgga tgcggaaaac gcagcagtta tttccaaaat gctgcgcgtg
    27121 atgatgctgg ctccgttcct catcctgctg gcggcgcgtg ttaaacagct gtctggggcg
    27181 aacagcggcg agaaaagcaa aatcactatt ccgtggtttg ccatcttgtt catcgtagtt
    27241 gccatcttta actcgttcca cctgttaccg cagagcgtgg tgaacatgct ggtaacgctg
    27301 gataccttcc tgctggcaat ggcgatggcg gcgctgggtc tgaccactca cgtcagcgcg
    27361 ctgaaaaaag ctggggcgaa acctctgctg atggcactgg tactgtttgc ctggctgatt
    27421 gttggtggtg gtgcgattaa ctatgtgatt caaagcgtca tcgcataaac cactacatct
    27481 tgctcctgtt aacccgctat cattaccgtt ttcctccagc gggtttaaca ggagtcctcg
    27541 catgaaatac attggagcgc acgttagtgc tgctggcggt ctggcaaatg ccgcaattcg
    27601 cgccgccgaa atcgacgcaa ccgcgtttgc cttgttcacc aaaaaccaac gtcagtggcg
    27661 tgccgcaccg ctcacgacgc aaaccatcga tgaattcaaa gccgcctgtg aaaaatatca
    27721 ctacacatcg gcgcaaattc ttccccacga cagttatctg attaacctcg gacatccggt
    27781 cactgaagct ctggaaaaat cgcgcgatgc ctttatagat gaaatgcagc gttgcgaaca
    27841 gctggggctt tctttgctca acttccaccc tggcagccat ctgatgcaga tttcagaaga
    27901 ggattgcctt gcgcgtattg ccgaatccat caacattgcg ctggataaaa ctcaaggtgt
    27961 gacagcggtg atagaaaaca ccgccggtca gggcagtaac ttagggttta aattcgaaca
    28021 tctcgcggcg attatcgacg gcgtggaaga taaatcccgc gtcggcgtct gcattgatac
    28081 ctgccatgct ttcgctgccg ggtatgattt gcgtactcca gccgaatgcg agaaaacatt
    28141 cgcggatttt gcccgtactg tcggctttaa gtatctgcgc gggatgcacc ttaacgatgc
    28201 gaaaagcacc tttggcagcc gcgttgaccg ccatcatagc ctcggtgaag gcaatatcgg
    28261 tcatgatgcg ttccgctgga tcatgcagga cgaccgtttc gacggcattc cgctgatcct
    28321 cgaaaccatc aacccggata tctgggcaga agagatcgcc tggctgaaag cgcaacaaac
    28381 tgaaaaagcg gtagcctgaa gatgaataac cgggaaaagg agatccttgc aattttacgg
    28441 cgtaacccgc tgattcagca gaacgaaatt gcggacatgc tgcaaatcag ccgttcgcgc
    28501 gttgcggcgc atattatgga tttaatgcgc aaaggccgga ttaaaggcaa aggttacatt
    28561 ctcaccgagc aggaatactg cgtagtggtg gggacaatca atatggatat tcgcgggatg
    28621 gcggatatcc gttacccgca atcggcttct catcccggta caattcattg ctcagcgggc
    28681 ggcgtgggac gcaacatcgc ccacaatctg gcgctgttag gccgtgacgt ccatttgctt
    28741 tcagtgattg gcgatgactt ttatggcgaa atgctcctgg aagaaacgcg ccgcgccggc
    28801 gtgaatgtct ccggctgcgt tcgtttacat ggtcaaagca catcgacgta tctggcaatt
    28861 gccaatcgag acgatgaaac cgtgctggcg attaacgata cccatctgct ggaacagtta
    28921 tcgccgcaat tattgaacgg gtcgcgcgat ttacttcgtc atgcgggcgt ggtactggct
    28981 gattgcaacc tgacagccga ggcgctggaa tgggtcttta ccctcgctgg tgagatcccg
    29041 gtgtttgtcg ataccgtttc agaattcaaa gcgggcaaaa tcaaacactg gctggcgcat
    29101 attcacaccc tgaaacccac tttaccggag ctggaaattt tatggggaca ggcgatcacc
    29161 agcgatgctg accgtaatgc tgcagtgaat gcgttgcatc agcaaggtgt tcagcaactg
    29221 tttgtttatt tgcccgatga gtcagtttat tgcagcgaaa aggatggaga acaatttttg
    29281 ctgaccgcac cagcgcatac gacagtagac agttttggtg ctgacgatgg ttttatggcg
    29341 ggcctggtat atagctttct ggaaggaaac aatttccgtg acagcgcccg ttttgcgatg
    29401 gcctgcgcgg caatttcacg cgccagcggc agcttaaaca accctaccct gtctgccgat
    29461 aacgcgcttt ctttagtgcc aatggtgtaa caatgttgcc ggattcaacg cttgaccgca
    29521 tcttatccgg ctcataaatc gcacactacg ctaaaccaat aaagaacccg gcaatggtgg
    29581 cactcatcag gttagaaagt gtcgccgccg ccagcgcgcg taaaccaagc tgggcgattt
    29641 ccggcgcacg gtgtggcgca accgcagaaa acgcccccac caccaccccg atagaaccaa
    29701 agttagcgaa accgcacaac gcgaaggaaa taatcgccac ggttttagca tccagagtgc
    29761 cagccgtttg cagatagggt gagaaattga gataagcgac aaattcattt attgccagtt
    29821 tctgtccaat caaactcccg gcaagattcg catcactcca gtcaacaccc attacccacg
    29881 ccagtggggc cagcagataa cctacaatgg actccagcga ggcatgttca aaaccaaacc
    29941 agccgccaac gccgccgata ataccgttaa ttaacgcaat gatggcgacg aatgccatca
    30001 ctactgtcgc cacacctgcg gcgattttca gcccggtcat tgcccctgtc gcggcggctt
    30061 caataatgct ttttggcggt gtttcggtga aagagaggtt attaaaagaa acctgcgaag
    30121 attccgtagc cgggcttagc aggcgggcaa acaagatccc cccggggatc gccattaacg
    30181 atgccgccag caaatattcc acaggcacgc ccagtgcggc ataaccaatc attgtcgaac
    30241 cagcaatcga ggccatgccg ctacaaatcg ctgtaaataa ttcattgcga ttcagccgat
    30301 cgataaaggg cttcacaatc gccggaattt cgttttgccc gaggaaaatg gtagtgaccg
    30361 cgacgaatga ctcaatcttg ctgatattta atgctttctg gaatataccg ccgagaatgc
    30421 gaattaaaat ccccatcaca ccgatgtagt agagaatact caccagtgca gtgacgaaga
    30481 taattgccgg taataccctg aaaccaaaga taaatcctgc gccatcaaat aaggtgtcca
    30541 ttttcggtcc gaccagagaa ccgaagataa atgcgctacc cgcgtcgctg tacgccatca
    30601 ctttatgcac accaaaagcg actttttcag cgacccaacg ccctggcggt aaccaaagca
    30661 taatgccgcc aatcacgacc tgtaacacta acgccgcgcc aacggtacgc aggctgatct
    30721 tcttcttgtt tactgacagt aaaaacgcaa tcgtcagcaa taccaccatt cccagaacac
    30781 ttctcatgac atccatagtg attatctctt catgtcatat tgggcgacgg ctttctgccc
    30841 atcagaaata gccaggcctg catcttgcga taccggcccg gcttgttatc aacacttaat
    30901 gggttttgat gtagccgcgc acgcactctt cgactaagcc ccagaaccag tcgctatcaa
    30961 tagtgatgcc gactttggta ttagcgggct tgccaagaac gcccagctcg tcgcagacgg
    31021 tacgcccata acaagggcca ctgttgacgt ccacttcgac gtacatctcc tgggttttaa
    31081 tgccatcagg gttaatcaga taaccgatgc aggtggcgtc gtgcaccggg ccgccagcca
    31141 ggccgtagtt ttcgaactgg gttttgagag tgaagttcat gatgtcgctg aacagctctc
    31201 cggcagggcc gcctgccctt tccattcgtg caatcacgtc cggggtgcaa acggtctggt
    31261 tggtgagatc gaggcccatc atcactaatg gaacgccaga ggtgaacacc acgcgtgcgg
    31321 cttccgggtc agcaaagatg ttgaactcgg cagatggcgt gaagttgcct gtaccgtaag
    31381 cgccgcccat tagcacaatt tcacggattt tgggcaggat cgcgggttgc atacgcattg
    31441 ccaccgcgat atttgaaagc ggaccaaccg gcaccagagt gatatcgcca tcgcttgcca
    31501 tcagggtatc aatgatgtat ttcaccgcat gagtgctttc tgcctggcgg gtcagcggct
    31561 cgaataccgg gccatccagt ccggtatcac cgtgaatatt atcggcaacg atttgttgcc
    31621 gcataatggg ttgtggcatc cccgcataaa ccggaacatt aatctccagt ttctggcaaa
    31681 cattcaggcc attaattaat gttttatcaa gcgtctgatt acccgctaca atagtgatgc
    31741 ctaataaatc tattgccgga tgtttcgccg ccatcattat agcaatagca tcatcatgac
    31801 ccggatcaca atccagaata atttttctct tttccattgt ttatttcctc tgtttccagt
    31861 tgcgttattt tttctacagc aaagaaattt cgatcgggcc gatgattgaa tcttaacaac
    31921 agcgtacgta tgctaaatat gagaaatctc atagcggata aacatcgtga aagaaatcca
    31981 caataatgat cttaagcagc aattgatgag tgaatccgcg tttaaggatt gctttttaac
    32041 ggatgtttca gccgatacgc ggctgtttca ttttttagcg cgtgactaca ttgtgcagga
    32101 agggcaacag ccgtcctggc tgttttacct gacgcgaggc cgcgccaggc tttacgccac
    32161 gctagctaat ggtcgcgtgt cgctgatcga tttctttgcc gccccctgtt ttattggtga
    32221 gattgagtta atcgataaag accatgaacc gcgtgcggta caggctattg aagagtgttg
    32281 gtgccttgcg ctccctatga aacattaccg tccgctgtta ttaaacgaca ccctattttt
    32341 acgaaaactt tgcgtcacct taagtcataa aaattatcgt aatattgttt ctttaactca
    32401 gaatcaatca tttccgttag tgaatcgcct ggcagcattt atattactct cacaagaagg
    32461 tgatctttat cacgaaaagc atacgcaagc ggcagagtat ttaggcgttt cttatcgaca
    32521 tcttttatat gttctcgcgc aattcattca cgacggttta ttaattaaaa gcaagaaagg
    32581 atatctaatt aaaaacagaa agcagttgtc agggctggca ctggagatgg acccggagaa
    32641 taaattctcc gggatgatgc agtaaaaatt atttgcaata gcgcgattgc cggatgcaac
    32701 gcttgatgcg ttttatccgg tctacaaatt gagcattacg ccagaccaat aaagaacccg
    32761 gcaatggtcg cgctcatcag gttggaaagc gttgctgctg ccagcgcccg taaaccaagc
    32821 tgagcgattt ccggtgcgcg ttttggcgaa atagccgaaa atgccccaac gacaacaccg
    32881 atagaaccaa agttagcaaa accacaaagc gcaaaggaga taatcgcaat ggttttcact
    32941 tccagcgtgc cgcccgtttg caggtatggg gagaaactca ggtaagcgac gaattcgtta
    33001 atcgccagtt tctgcccaat caggctaccc gcaaggttgg catcactcca gtccacaccc
    33061 atgatccacg ccagcggtgc cagcacatag ccaaaaatac tttccagaga agcattggcg
    33121 aagccaaacc agccgccaat tccgccgata atgccgttga tcagcgcaat aattgccaca
    33181 aatgccatta ccaccgtcgc cacaccagcg gcgattttca gcccggtcat cgcaccgctc
    33241 gccgctgctt cgataaagct ttttggcggc gtttcgctga acgacagatt ttcaaacgtg
    33301 acctgcgaaa gctcggttgc tggactaaga atacgtgcaa acaaaatacc gccggggatc
    33361 gccatcagcg atgccgccaa caggtagtca attggtacgc ccattccggc ataaccaatc
    33421 atcatcgaac cagcaatgga cgccatcccg ctacaaatag ccgtaaacaa ctcgtttcga
    33481 ttcatgcgat cgataaacgg tttaacgatc gccgggatct cattttgccc gaggaaaata
    33541 gtggtgactg cgacaaaaga ttcgattttg ctgatgttga gggctttctg gaaaatgctg
    33601 ccaaggatgc gaatcagcag ccccatcacg ccaatgtagt acagcagact aatgagcgca
    33661 gtgacgaaga taatcgccgg aagtacgcga aaggcgaaga taaaacccgc accgtcaaag
    33721 aggacatcca ttttcggccc gaccagcgaa ccaaaaatga aggcgctacc agcatcactg
    33781 taagacatca ctttatgaac gcctaatgcc gcctgttcta cagcccactt tcccggcgga
    33841 aaatagagca tgatgccacc aagagcaatt tgcagcagta gtgcggctgc aaccgtacgc
    33901 aaactgatgc tctttttatt cactgacaac agaaatgcta ttgccagtaa caccaccatc
    33961 cccacaacac ttctcattat gtccataatg attttccctt catgccagta aacccggcgt
    34021 cagcgccagg tttcggtatg tttgatgagt acgggcgacg gctttctgcc cgtcagaaat
    34081 taacccgcga gacgctgata ttctttggca atttcgctcg ccagaatggc gttgttgaac
    34141 acaagctgga tgttggattt caggctgtca ccgccggtca gctcagcaac gcgcgccagc
    34201 aggaatggcg tactttcttt accaataaca ccctgcgctt cagcttcagc taccgcctga
    34261 tcgatcaccg cattgatagt gtgttctggc atcgcaaact gttccgggat cgggttcgct
    34321 accacgaggc caccgttcag cccgctttgc catttcaccg ccattgcacg ggcaatttca
    34381 ctggcgctgt cgagacgaat gctgacgtca aacgggctgg tacggcagaa aaacgcaggc
    34441 agcgctttag tctgatagcc aattaacggc acaccgaagg tttctaaata ctcagtggtt
    34501 aatcctaaat cgagaataga tttcgccccg gcacaaacaa cggtgacatt agtatttgcc
    34561 agttcttgca aatcggcaga aatatcgaag gtatgttccg cccctcgatg aacaccacca
    34621 attccgccgg tggcaaatac tttaattccg gcaagcgccg caataatcat cgttgaagcc
    34681 acggtggttg caccattttt tcctgcggca acgacaaaag gtaaatcgcg acgactaact
    34741 ttagtcacgt tatgcccttc acgacccagt aattcaattt cttctttgct taaacccact
    34801 ttcatcacgc cgccaataat ggcgatagtg gcaggtactg cgccctgttt acgaatagtt
    34861 tcttctactt caattgcggt ctgggcattt tgtgggaacg gcatcccgtg agaaataatg
    34921 gtcgattcca gcgccacaac cggttttttg ttttttaaag cgtcctgcac ttccggggaa
    34981 atttttaatt cagacattct gcgttctcca ctaacgatat aacgttggca atcgataaat
    35041 cggggttatt ggtgtattca caggagagcg ccattgacga acatccctgt gcgaaacgaa
    35101 cagattcggc aaacggcatt ccgtctaccc aacacgaagc aagtcccgcc atcatggcat
    35161 cgcccgctcc ggtaacatta ataacattgg ttttgatcgg cgcagaccag ccactttcac
    35221 cgctgatatc gctgtaataa acgccgtcgc cgcccatgct caataccagt cggttcaggc
    35281 catgttgatg gaaccaggca gcaacttttg ccacatcttc acgccctgac agcgcaatcc
    35341 cactcagggt ttccgcttca aggcggtttg gcttgagagt atggatttga cttaatcgct
    35401 cgcgtacttt gacgcatttc cacgcggaaa ccggatcgac aaatacgggt acgttggcag
    35461 cattatccag aatccatgcc agtgcctctt cactgatatt acagtccgcg acaatgacct
    35521 ttgccctctg aataaattca cggtgctgtg cgagatattc agctgtaata gcgttgctaa
    35581 tattcatgtc atttatagca accagcattt caccggtatt atcgagtaat gataaatagc
    35641 tcgacgtatt ttctcccggc acaatcaggc atttatcgac ataaacgcca gattgattgg
    35701 tttgcgttag cagcgattgg ccataaaaat cactgcctac ggcgctcagt agccaggctt
    35761 tattacccag caacgccagg ttttgtgcaa tattgcgccc taccccacca ggcgtaaatt
    35821 ttattttacc tggatttgaa tccgcataat ttaatgattc atgtgaatat ccggcgacat
    35881 caatattcgc cgagcctata attacgacat aatccttttc gcgcatagca cgtcctctgg
    35941 taaaaaatta ccaaccctgt caatccacaa ggatatagat actgcaaaga cggtttaaac
    36001 atgtgttcat atttaaacat gtgctcatag tatgcatgtt ttacgtaaag taaattgccg
    36061 ccagagaggc aaagcgggca gatttgagca ggctcacaaa atgcgattaa atgcctgaat
    36121 tacaaaagcg aggaatttgt gacgggatgc acagaacatg aacactaaaa aagggcagaa
    36181 aagaatctgc ccttgaggaa ataaccttag tgtttgatga acgtttcagg tttctactga
    36241 aagataaaac catttaaatt cataaaatta cattcacaaa ccggtcattc tctttgcgct
    36301 catccaatga tacggggcag taaatttctg ccctgtaaca caccttttat tacgcggctt
    36361 tcgctactgc gtccacttcc ggacgtttca ggaaggcata ggccaaaccc gccaccagcg
    36421 taccggcaat aattgctacc aggtaaccca gtaccggcgt aatagcgcca gggatcagca
    36481 gaacaaacag accaccgtgc ggtgccatca gtttcgcacc aatcgccatt gagattgcgc
    36541 cagtcagcgc cccaccaacg atacaacacg gcagcacacg catcggatca cgagcagcaa
    36601 aaggaattgc accttccgaa ataaagcaca gaccaagaac cagagcggct ttgccacctt
    36661 cctgctgcgc tttgtcaaat ttgcgacgcg ccaccattgt tgccagaccc attgccagcg
    36721 gtggcaccat acctgccgcc ataatcgccg ccatcgggcc ataggtttga gtactcagca
    36781 gacccacacc gaatgcgtac gctgctttat ttaccggacc gcccatgtca gtacacatca
    36841 tgccaccgag gatcgccccc agcagaaccg cattcgcagt ccccatggtc tgcagccagt
    36901 gagtcaaccc ttcgagaatg ccagcaaccg gtttaccgat caggtagatc atcgccagac
    36961 cgaccaccag actggaaatt agcggaatga tcaggatcgg tttcagcgcc tccatactct
    37021 gtggcagttt cagttgcgta ctgattaact tcgcaatgta accagccagg aagcccgcaa
    37081 taataccgcc aatgaagcca gaaccggtgc tgaccgccag cataccgcca atcagacccg
    37141 gcgtgagacc cggacgatcg gcaatggaga aggcaatata acctgccagt accggcacca
    37201 tcagcgcaaa ggctgaacca ccaccaatct gcatcagcgc cgctgccaac gtacccggct
    37261 ctttaaacgc ttcgatacca aaagcaaaag aaagcgcgat acacagacca cctgcaacga
    37321 ccatcggcag catgtaagag acgcccgtca gcaagtgacg gtatgcgcct gcactctcgt
    37381 tcttaccttc agaggtcgcc gtttgagctt tgcccgccgg ttcatacggc gttgcttcag
    37441 caaccgcttt atccagttcc tgcgcggttt tcttcagcgc cagaccggta gaggtgcgat
    37501 acatcggttt accagcaaat ttcgccagat ccacttcgat atctgccgcc acaatcacca
    37561 gatccgctgc ggctacttct tcgggagtga ttgcattacc cgcgccaaca gaaccacggg
    37621 tttcaacttt cacccaccag ccacgttttt tcgcttcggt ttcaatggct tcagccgcca
    37681 taaaggtgtg tgctacgcca gtcgggcaag cagtcaccgc aactacgcgt ttcggaccgc
    37741 tggcggcaac cggtgctgtc gcagcgaccg gcgcagtgta aggtttcgca tggcctttgg
    37801 cttcactcag gaacagctca gggtgcgcaa ctgcccggga aatatcgccc agccagacat
    37861 ttttaccgtt cagcgcgctg tcattcggga tggaatcacc gagaacaatc gccatttctg
    37921 cgtcgttcgg attgtcgatg atttccagtt ttgcttttcg cgccgccgcg cccagcaggg
    37981 tcttcgccat ataggcgcgt gcctgaccga gattagcgtc aataatcagc agcgttttca
    38041 ttatgcctct cctgctgtca gttaaaaggt tgtaagtcga cgcgcgccat cattgcggcc
    38101 aactgcggac gatcggtaat acccacattg ctttgactta ccgccagggc tgcaacagca
    38161 gtcgccagac gcagtgtgtg ttcactggat tcacgcatta gcaagccata aatcaggcca
    38221 ccaaccatag aatcccctgc gccaacggtg cttacgacat cgactgacgg tggtttggcg
    38281 ctccattcgc cggaggcatt aacccaaagc gcgccttcgg cacccagtga aataacgaca
    38341 tgcgcaatac cttgttcacg cagcgcatgc gcagcttcaa tcacatcttt catttcaggc
    38401 agtttacggc ctgcccagat ttccagctcg cggcggttag gtttcaccag ccacggtgcc
    38461 gctttcaaac ctgctactaa cgcttcacgg ctactatcaa agataatgca agggcactga
    38521 ctacgcaggc gagtcatcca gtcggtgaac gcttccgggc tgacgcctga cggtaagctt
    38581 ccgctgacac agaccatatc gaactgaccg agccagctca gagaatcagt cacaaagcgt
    38641 tcccagtcgg cgggggtgac ttcaaaaccc gagaagttga agtcggtcac ttcgccgtct
    38701 ttttccgtca gcttaacgtt aattcgggtg cgcccctgta caacctggaa acggttggca
    38761 atgcccagct cgctgaacag ttgctgaaaa ccatcctgat tgtctttacc gaggaagccg
    38821 ccaacggtga catcaattcc caggtctttt aatactttag ccacgttgat gcctttaccc
    38881 gccgcatgca gaccggtggt tttcaccagg ttcacttcgc cgcgttcaat ttccgggcag
    38941 aaaccaacaa ggtcataagc cggattaagg gtgatagtag caacacgtct gctcattatg
    39001 cgccctcccc aagaccagca gcgatagcgt cgccgattgc tttcagcgcc tgttcagcat
    39061 ctgcaccctg ggcggtaaag cgtaggcgat gacctttctt aacgccaagt gccacaactt
    39121 tcatcagact acgtccgttt gccggtttgc cggtgccatc aaggtttgtc acggtaatat
    39181 cactgttaaa ttgtttaatg gtgttgacca gcatggtacc tggacgagca tgcaggccgt
    39241 gttcattgcg caccacaaac tccgcgctta acacgtcgtc ggtcggcgca tcatcgctgg
    39301 tcagcagcgc cagcaacgtt gccgcatccg ctttcagcaa gcggtcagct ttattgtcga
    39361 gcaacaaatc agcgagacgc ttaagaaccg caatgggctg atcgtcattc atcgccacac
    39421 tcaccagcat ggctgccgtt tcgccgtcca catcaaaagc atttgccgca cggcttaccg
    39481 caattgcgct acgcagattg ccttcggcgc tatcgctcag ccagataccc tgtccgaggt
    39541 tcagcggttg ttcattgatg gctttggtga cgaaagtggc gtcaactgcc cccgcctctt
    39601 tcagacgcgc agcgttcagc gcctgaagag tcagcagatc gctggcgacg atatccagcg
    39661 tcagcatttc gttgtcgagc ttcagctgct cactctgctt ttcgcccatc agcaatgcgc
    39721 gaagttcttc tgctgttgtt gctgacttca gttgttcaac aacggaatca tcgctcagta
    39781 cgtgggtcag ctggcgtagc aggcccagat gctcatccga gctggcagca ataccgatcg
    39841 ctacgtacgc tacctgaccg tcaccccagg tgacgccttc cgggaactga aatacctgaa
    39901 cgccggtttt cagcacctga tcgcgggtgt cggtagtgcc gtgtggaata gcaataccat
    39961 tgccgaggaa cgttgaggtt tgctgttcgc gcgccagcat gccattgacg tagccttctg
    40021 ctacattacc ggcctgcacc agcgccgcag cgacctggcg aatcgcctct tctttgtctc
    40081 cggccttttc gcccggatgg atgtcctgta cggataactg gaacatagtt ctcctctctt
    40141 gctgaattga aacgattcag cttctatgag aaaaaaagcg ccaacctggc ttagggttaa
    40201 agacaagatc gcgctgaaac gtttcaagaa agcataatac ttctgtttca gcacgcaagg
    40261 aaagccgtaa agttatgagc aaaagtttga tgtgctgcac aattttttgt catttttcct
    40321 aattgctgat gggaaaatcc gttatcagca atttcatttc agcagcgtca gcagccaatc
    40381 agcaacagtg aaatggcatt ttgattattc gtggcgcttt tgatttcgct gtctgtttat
    40441 tttctgacaa gcagcgtaaa ctccgcgtct tcctcttcca gtgatcgacc agcatgcata
    40501 actcccccgc agtctccagc gcgaaatcgt ttgacctgac ctcgacggcg tttttaatcg
    40561 ttgcctttct caccggtatt gcgggcgctc tgcaaacccc gacactcagt atttttctta
    40621 ccgatgaagt acatgcccgt ccggcgatgg tgggattctt ctttaccggc agcgctgtca
    40681 ttggggttct ggtgagtcag tttctcgccg ggcgctctga taagcgcggc gatcgcaaat
    40741 cgctgattgt cttttgctgc ctgttaggcg tgctggcctg cacccttttt gcctggaatc
    40801 gcaactactt tgttttgcta ttcgttggcg tctttcttag cagctttggc tcgaccgcta
    40861 acccgcaaat gtttgccctt gcccgtgaac atgccgacaa aaccggacgt gaggcggtga
    40921 tgttcagctc ttttttacgc gctcaggttt cactggcatg ggtcattggc ccaccgctgg
    40981 cttatgcctt agcgatgggt ttcagcttta cggtaatgta tctgagcgca gcggtagcat
    41041 ttattgtttg cggcgtgatg gtgtggctgt ttttaccgtc gatgcaaaaa gagcttccgc
    41101 tggcgaccgg cacggttgaa gcgccgcgcc gtaaccgtcg cgatacgctg ctgctgtttg
    41161 tcatttgtac attgatgtgg ggctcgaaca gcctgtacat catcaacatg ccgctattta
    41221 ttatcaacga actccatctt cccgagaaac tggccggtgt gatgatgggg accgccgccg
    41281 ggctggaaat cccgaccatg ttgattgccg gatatttcgc caaacgtctg ggtaagcgtt
    41341 tcttaatgcg cgttgctgcc gtgggtggcg tctgttttta cgcaggaatg ctgatggcgc
    41401 attctcctgt cattctgttg ggcttgcagc tgctaaatgc tatttttatt ggcattctgg
    41461 gcggtatcgg gatgctctat tttcaggatc tgatgcccgg tcaggcaggt tcagccacca
    41521 cgctctatac caacacgtcg cgcgtgggct ggatcatcgc gggatcagtg gctggcatcg
    41581 tcgccgagat ctggaattat cacgctgtgt tctggtttgc gatggtgatg attatcgcca
    41641 ctctgttttg cttactgcgg attaaagatg tttaaggcgc ggtcagcatt tccagatcga
    41701 taagccaggt cattgcctgc tgacgcgagt taccgcacat ttcagcgctg gcttgtaagc
    41761 ctgcacagac tttcggccgc agtggcgagg tgaaaatttt acagcgttgc tgttcatcaa
    41821 gctgaatgca gggcgtattg gcgggcttgc catctggcat accgggaata gggctggaaa
    41881 ttgaaggggc agtgcaacac gccccgcaac ccggacggca ttccatacgt tgttctctta
    41941 aggttaacca ccggattgat gagcgcgcac agtaccacct tttttgcacc agcaaaagtg
    42001 cgaataccac ttgcccgaaa ggctcgtcgc gagtactttg tcgcgatatt tttgacattt
    42061 tcgactacag gaatttttcg atgccaagag cgaacgaaat taaaaaaggt atggtactga
    42121 attacaacgg caaactgctg ctggttaagg acattgatat tcagtcgccc actgcccgcg
    42181 gtgccgctac gctgtacaaa atgcgttttt ctgatgtccg taccgggctg aaagtagaag
    42241 agcgtttcaa aggagatgat atcgttgaca ccgtgacgct gacccgccgt tacgttgact
    42301 tctcctatgt cgatggcaac gaatatgtct ttatggataa agaagactat accccgtata
    42361 ccttcaccaa agatcagatt gaagaagagt tgctgtttat gccagagggc ggcatgccgg
    42421 acatgcaggt gctgacctgg gacggtcaac tgctggcgct tgagctgccg cagaccgttg
    42481 atctggaaat cgttgaaacc gcaccaggca tcaaaggggc atctgccagc gcccgtaaca
    42541 aaccggcgac attgagcact ggtctggtga ttcaggtacc agaatactta agcccgggcg
    42601 aaaaaattcg tatccatatc gaagaacgcc gttatatggg gcgtgctgac taacttcagc
    42661 cgcatgcaga aaagggatag ctcaggctgt ccctttttta atttattata ccaatcttct
    42721 attttgcgct tcctgaacac cttacgccct caattttcac tcgttgattg atcgccctca
    42781 cacttcatcg cattaacaat ccagaccaat ttcaattgct gtcatataac tttacactgt
    42841 cattgttaat taatcgttac taagacatga ctcctatgaa tacaatcgcc tccgttacgc
    42901 tcccgcatca tgtacacgct ccacgctacg atcgccagca gttgcaatca cgtatcgttc
    42961 attttggctt tggagccttt caccgcgcac atcaggcgtt actgaccgat cgtgtgctga
    43021 atgcccaggg cggcgactgg gggatctgtg aaatcagctt gttcagcggt gatcaactga
    43081 tgagccagct ccgcgcacag aaccatttat ataccgtgct ggagaaaggg gcggacggca
    43141 atcaggcgat aattgtcggt gccgttcacg aatgcctgaa tgcaaaactg gattccttag
    43201 cggcaattat tgagaaattt tgcgagccac aggtggcaat tgtttccctg acgattaccg
    43261 aaaaaggcta ttgtattgac ccggccaccg gtgcactcga caccagcaat ccgcggatta
    43321 ttcacgatct acaaacccct gaagaacctc actccgcacc gggtattctc gtcgaagcac
    43381 tgaaacgccg ccgtgagcgc ggccttacac cgtttaccgt gctctcctgc gacaatattc
    43441 ccgacaatgg ccatgtggtg aaaaacgcgg tgctgggaat ggcagaaaaa cgttcgccag
    43501 aactcgccgg gtggataaaa gaacacgtca gttttccggg aaccatggtc gaccgcattg
    43561 ttccggctgc aaccgacgaa tcactggcag aaatcagcca gcatctgggg gtaaatgatc
    43621 cctgcgcgat tagctgcgaa ccgtttatcc agtgggtggt ggaagataac ttcgtcgctg
    43681 ggcgtcctgc ctgggaagtc gcaggtgtac aaatggtgaa tgatgtcctg ccatgggaag
    43741 agatgaaact gcggatgctt aatggcagcc actcttttct cgcttatctg ggttacctct
    43801 caggattctc ccatatcagt gattgcatgc aggatcgcgc atttcgccat gccgccagaa
    43861 cattaatgct ggatgagcaa gcgccgacac tgcggattaa agatgtcgat ttaacacaat
    43921 atgcggataa gttaattgca cgttttgcta atccggcgct gaaacataag acctggcaaa
    43981 tcgcgatgga tggcagccag aaattaccgc aacgcatgct ggcaggtatt cgcatacatc
    44041 tggggcgcga aacggactgg tcgttgctgg cattaggcgt tgcaggctgg atgcgttacg
    44101 tcagcggcgt tgatgatgcc ggaaatgcca ttgatgttcg cgatccgctt agcgataaaa
    44161 ttcgcgatct tgttgcgggc agcatcagtg agcaacgcgt caccgccctg ctttccctgc
    44221 gtgaagtttt cggtgatgat ctgccagata acccgcattt tgtgcaggcc atcgaacaag
    44281 cccggcaaca aatcgcacaa ttcggcgcgc atcaggcgct attaaacacc ctcaaaattt
    44341 aacgatttct gcggttaaag cggatgaagc tcgccttcgt ccgctctccc cttctctttt
    44401 ctgccgtttt tagccaggat taacgctcag ttaacttacc agagtattgc ggagctgttg
    44461 tgaccaggac caacctcatc accggttttc tcggcagcgg gaaaaccacg tcgattcttc
    44521 atctgttagc ccataaaaat cccaacgaaa aatgggcggt actggttaat gaatttgggg
    44581 aagtcggaat tgatggtgct ttgctcgccg atagcggcgc actgctgaaa gagatccccg
    44641 gcggctgcat gtgctgcgtt aatggtttac ccatgcaggt agggttgaat accttactgc
    44701 gtcagggaaa accagaccgc ttgttgatag agccgactgg gctgggccat ccgaaacaga
    44761 tcctcgatct gttaaccgca ccagtctatg aaccgtggat agatctgcgc gccacgttgt
    44821 gcattctcga tccacgcctg ctgctggacg aaaaaagcgc cagcaatgaa aacttccgtg
    44881 accagctggc tgccgcagac atcattgtcg ccaataaatc cgaccgtgcg acacctgaaa
    44941 gcgagcaagc gctacagcgc tggtggcagc aaaatggtgg cgatcggcaa ttaattcaca
    45001 gtgagcatgg gaaagttgcc ggtcatcttc tggatttgcc gcgtcacaat ttagccgagt
    45061 tgcccgccag cgccgcgcat tctcatcagc atgtcgtgaa aaaagggtta gcagcgttaa
    45121 gcctgccaga gcatcaacgc tggcgtcgca gtctgaacag tggacaagga catcaggcct
    45181 gcggctggat attcgacgct gatacggtat tcgacaccat tggcattctg gaatgggcgc
    45241 gacttgcacc ggtggaacgc gtcaaaggcg tgctgcgtat tcccgaaggg ctggtgcgaa
    45301 tcaaccgtca gggcgatgac ctgcacattg aaacgcaaaa cgttgcgcca ccggacagcc
    45361 gtattgagct gatttccagc agcgaagctg actggaatgc cttgcagagc gcgctgttga
    45421 agcttcgttt agcgactacc gcgtaaggtt gcctgcgttt ttcagtaaga taattagaga
    45481 aaatatgatt aaaaatttgc cgcaaatagt gttgttgaat attgtcggcc tcgcgctgtt
    45541 tctttcctgg tatattcccg ttaatcatgg attctggttg ccgattgatg cggatatttt
    45601 ttatttcttt aatcagaaac tggtcgaaag taaggccttt ttgtggctgg ttgcattgac
    45661 caacaatcgc gctttcgacg gttgttcact gctggcaatg ggaatgttga tgctgagttt
    45721 ctggctgaaa gaaaacgccc caggcagacg acgtatcgtg attattggtc tggtcatgct
    45781 attaactgca gtggtattaa accagctggg tcaggcatta attcctgtaa aacgggccag
    45841 cccaacattg acttttaccg atattaaccg cgtcagcgaa ctgctctctg tccccacgaa
    45901 agatgcctca cgagatagct ttcccggcga tcacggcatg atgctgctta ttttttcggc
    45961 attcatgtgg cgttatttcg gcaaagttgc aggtcttatc gcccttatta tttttgtggt
    46021 ttttgcgttt cccagagtaa tgattggcgc acactggttt actgacatca ttgtcggttc
    46081 gatgaccgtg atattgatcg gtttgccctg ggtgttgctg acgccattaa gtgatcgatt
    46141 aatcaccttt tttgacaaat cactaccggg aaaaaacaaa catttccaaa acaaataact
    46201 cacagtactt aacatcatca ggtttatttt tatagtgagg ataatcctga tgatgcgcac
    46261 cgtgctttca tccatcgaaa gcaaaaatca ttctctaagt aaatgaatgg attgcatgca
    46321 tttcactcaa tcgtacttta attgaccaat cccgcttatt aactttctgt atcacttttt
    46381 cttataaaaa atcatgtaaa accgctcgcc aagaccgcac caatcgggta atctcgaact
    46441 cgttttgcct cggcggtaga ttatcctcac agcatataat tttgtgcgtt agtccacaga
    46501 tttggcctta aggaattgtt tcaacatgcc ctggtaatta gtctcgtgtc gcttggcatt
    46561 ttttttataa cgatatttgt cgttaaggac ttcaagggaa aacaaacaac atggtcaaat
    46621 ctcaaccgat tttgagatat atcttgcgcg ggatccccgc gattgcagta gcggttctgc
    46681 tttctgcatg tagtgcaaat aacaccgcaa agaatatgca tcctgagaca cgtgcagtgg
    46741 gtagtgaaac atcatcactg caagcttctc aggatgaatt tgaaaacctg gttcgtaatg
    46801 tcgacgtaaa atcgcgaatt atggatcagt atgctgactg gaaaggcgta cgttatcgtc
    46861 tgggcggcag cactaaaaaa ggtatcgatt gttctggttt cgtacagcgt acattccgtg
    46921 agcaatttgg cttagaactt ccgcgttcga cttacgaaca gcaggaaatg ggtaaatctg
    46981 tttcccgcag taatttgcgt acgggtgatt tagttctgtt ccgtgccggt tcaacgggac
    47041 gccatgtcgg tatttatatc ggcaacaatc agtttgtcca tgcttccacc agcagtggtg
    47101 ttattatttc cagcatgaat gaaccgtact ggaagaagcg ttacaacgaa gcacgccggg
    47161 ttctcagccg cagctaataa accgtttgga tgcaatccct tggctatcct gacgagttaa
    47221 ctgaaagcac tgtttaggca gtgctttttt gttttcattc atcagagaaa atgatgtttc
    47281 cgcgtcttga tccaggctat agtccggtca ttgttatctt ttaaatgttg tcgtaatttc
    47341 aggaaattaa cggaatcatg ttcatacgcg ctcccaattc tggacgtaag ctcctgctta
    47401 cctgcattgt tgcaggcgta atgattgcga tactggtgag ctgccttcag tttttagtgg
    47461 cctggcataa gcacgaagtc aaatacgaca cactgattac cgacgtacaa aagtatctcg
    47521 atacctattt tgccgacctg aaatccacta ctgaccggct ccagccgctg accttagata
    47581 catgcaagca agctaacccc gaactgaccg cccgcgcggc gtttagcatg aatgtccgaa
    47641 cgtttgtgct ggtgaaagat aaaaaaacat tctgttcatc tgcgaccggt gagatggaca
    47701 ttccactcaa tgaattgatt cctgcgctcg acattaataa aaatgtcgat atggcgatct
    47761 tacccggcac gccgatggtg ccgaacaaac ccgcaatcgt catctggtat cgcaaccctt
    47821 tgctgaaaaa tagcggcgtc tttgccgctc tgaatctcaa cctgacgcct tctctctttt
    47881 atagctcacg gcaggaagat tacgatggcg tcgccctcat tattggcaat actgcgctat
    47941 ctaccttttc ttcacgtttg atgaacgtta acgaattaac cgacatgcca gtccgtgaaa
    48001 ctaaaattgc gggcattcct ctgaccgttc ggctttatgc agatgactgg acatggaacg
    48061 atgtgtggta cgcattttta ctgggcggca tgagtggaac tgtcgttggc ctgctctgct
    48121 attacctgat gagcgtacgt atgcgccccg gcagagaaat catgaccgcc atcaaacgcg
    48181 aacaatttta cgtggcgtat caaccggtgg tggatacaca agctttgcga gtaacgggcc
    48241 tggaagtact gctacgctgg cggcatcctg tcgcgggaga aattcccccg gatgccttca
    48301 ttaactttgc cgaatcgcaa aagatgattg tgccgctgac tcagcacctg tttgagttaa
    48361 ttgcccgcga tgccgcagaa ttagaaaaag tgctgccggt aggcgtcaaa tttggcatta
    48421 acattgcgcc ggaccatctg cacagcgaaa gctttaaagc aaatatccag aaactgctca
    48481 cttccctacc cgcacaccat ttccagattg tgctggaaat taccgagcgc gatatgctga
    48541 aagagcaaga agccacacaa ctcttcgcct ggctgcactc ggtcggcgta gaaattgcta
    48601 ttgatgactt cggcaccggg cacagcgcgc ttatctatct tgagcgtttt acgctcgatt
    48661 atctgaaaat tgaccgtgga tttatcaacg ccatcggtac ggaaacgatc acttcacccg
    48721 tacttgacgc ggtgctgacg ctggcgaaac gcctcaatat gctgacggtt gctgaggggg
    48781 tcgaaacgcc ggaacaggcg cgatggctaa gcgaacgcgg cgttaatttc atgcaaggct
    48841 actggattag tcgcccgtta ccgctggacg attttgtccg ctggctgaag aaaccgtata
    48901 cgccgcagtg gtaaggtgtg cttacgtccc ttattattca tagtgaaagc atgccggatt
    48961 gcggctaatg atgagtaaaa ggaaatccgt tgcagatgat tgtgcgcata ctgctgctgt
    49021 ttatcgctct gttcaccttt ggtgcgcagg cgcaggctat caaggaaagc tatgcctttg
    49081 ccgtgctggg cgaaccccgg tacgcgttta atttcaacca ttttgattat gtgaaccccg
    49141 ccgcgccaaa aggtgggcag ataacgttgt cagccctcgg taccttcgat aatttcaacc
    49201 gctatgcact gcgcggcaac ccgggcgcac gcaccgagca gctgtacgac acgctattta
    49261 cgacttccga tgacgaacca ggcagttatt acccgctgat tgctgaaagc gcacgctatg
    49321 ctgacgatta ttcctgggtg gaggtcgcta ttaatcctcg cgcccgtttt catgatggtt
    49381 cgcccattac tgcccgcgat gtagagttta cttttcaaaa atttatgact gaaggcgtgc
    49441 cgcaatttcg tctggtctac aaaggcacca ccgtcaaagc cattgcgccg ttaaccgtgc
    49501 gcattgagtt agctaaaccc ggcaaagaag atatgttgag tctgttttcg ctgccggtat
    49561 ttccagaaaa gtactggaag gatcacaaac ttagcgatcc gctcgccacg cctccgcttg
    49621 ccagtggtcc gtaccgaatt acgtcctgga aaatggggca aaatattgtc tattcccgcg
    49681 tgaaagatta ctgggcagca aacttaccgg taaaccgtgg acgctggaat ttcgacacca
    49741 ttcgctacga ttattacctc gatgataatg tcgcctttga agcgtttaaa gcaggtgcct
    49801 ttgatttgcg tatggaaaac gacgccaaaa actgggccac acgctatagc ggtaaaaatt
    49861 tcgataaaaa atacatcatc aaagatgagc aaaagaacga atcagcccag gatacgcgct
    49921 ggctggcgtt taatatccaa cgtccggtat tcagcgatcg ccgggtccgg gaagcaatca
    49981 ccctcgcctt tgactttgaa tggatgaaca aggcgttgtt ttacaatgcc tggagtcgca
    50041 cgaacagtta ttttcagaat accgaatacg cggccagaaa ttaccccgac gccgcggagc
    50101 tggtgcttct ggcaccaatg aaaaaagatt taccgccaga agtcttcaca caaatctacc
    50161 agccgccggt atcgaaaggc gatggctacg atcgtgacaa cctgttaaaa gccgacaaac
    50221 ttctcaacga agcgggctgg gtgctgaaag gtcagcaacg cgttaatgcc actacgggtc
    50281 agccgctcag ctttgaatta ttgcttcctg caagcagcaa tagtcagtgg gtattgccgt
    50341 tccagcacag cctgcaacgg ctgggtatca acatggacat tcgcaaggtg gatcactcgc
    50401 aaatcaccaa ccgcatgcgc agtcgcgact atgacatgat gccgcgccta tggcgggcga
    50461 tgccgtggcc cagttccgat ttacagattt cctggtcatc ggaatatatc aattccactt
    50521 ataatgcccc cggcgtgcaa agcccggtta tcgactcgct gatcaaccaa attattgccg
    50581 cgcagggaaa taaagaaaaa ttactgccgt tagggcgagc actggatcgc gtattaacgt
    50641 ggaattatta catgctgcca atgtggtaca tggcggaaga ccgtctcgcc tggtgggata
    50701 aattctccca acccgctgtg cgccctgttt acagtctggg tatcgatacc tggtggtatg
    50761 atgttaataa agcggccaaa ctgccgtcag ccagacaaca gggagagtag atgggcgctt
    50821 atctgattcg ccgtctgttg ctggtgatcc caacgttatg ggcgattatc actatcaact
    50881 ttttcatcgt gcaaattgcg cctggcggtc cggtcgacca ggccatcgcc gccattgagt
    50941 ttggtaatgc cggagtatta cccggcgcag gcggtgaagg cgttcgcgcc agccatgcgc
    51001 aaacgggcgt cggtaatatc agcgacagta attaccgtgg cggacgcgga ttagatccag
    51061 aagtgatcgc cgagatcact catcgctacg gtttcgataa gccgatccac gaacgttact
    51121 tcaaaatgct ctgggactac atccgctttg attttggcga tagcctgttt cgcagcgcct
    51181 cggtgctgac gctgattaaa gacagtctgc cggtttccat caccctcgga ttgtggagca
    51241 cgctgattat ctatctggtg tcgattccgt taggcattcg caaagctgtt tataatggga
    51301 gccgctttga cgtctggagt agcgcattta tcatcatcgg ctacgccatt ccggcctttt
    51361 tgtttgccat cctgctgatt gtcttcttcg caggcggcag ctatttcgac ctgttccctc
    51421 tacgcggcct ggtttccgct aactttgatt cgctgccgtg gtatcagaaa atcaccgatt
    51481 atctgtggca tatcacgctg ccggtgctgg cgacggtgat tggtggcttt gcggcgctga
    51541 ctatgctgac aaaaaactca ttccttgatg aagtacgcaa gcaatacgtg gtgaccgcgc
    51601 gtgcgaaagg ggtaagtgaa aaaaatattc tctggaaaca tgtgttccgc aacgccatgc
    51661 tgctggtgat tgccggtttt ccggcgacgt ttatcagcat gttttttacc ggctcgctgc
    51721 tgattgaggt gatgttttca ctcaatggtc ttggcttact gggctacgaa gcgaccgtct
    51781 cgcgcgatta tcctgtaatg tttggtacct tgtatatttt caccctgatt ggcctgctgc
    51841 tgaatattgt cagtgatatc agctatacgc tggtcgatcc gcgtatagat tttgagggac
    51901 gttaatgtcg cgactcaacc ccgtcaatca ggcccgttgg gcgcgttttc gccataaccg
    51961 tcgcggctac tggtcgttat ggattttcct cgttttgttt ggtttgagtt tgtgttctga
    52021 acttatcgcc aacgataaac cgttgctggt gcgttatgac ggcagttggt atttcccgtt
    52081 gttgaaaaac tacagcgaaa gcgattttgg cggcccgctg gcaagtcagg ctgattatca
    52141 ggacccgtgg ctgaaacaac ggctggaaaa taatggctgg gtactgtggg caccgattcg
    52201 ctttggtgct accagtatca actttgctac cgataagccc ttcccttctc ccccctcccg
    52261 gcaaaactgg ctgggaacgg atgccaacgg cggcgatgtg ctggcacgta ttctctatgg
    52321 cacgcggatc tcggttctgt ttggcctgat gctgactctc tgttccagcg tgatgggcgt
    52381 gctggcgggg gcgctacaag gctattacgg cggtaaggtc gatctctggg ggcagcgctt
    52441 tattgaagta tggtcgggga tgccaacgct gtttttgatt attctgcttt ccagcgttgt
    52501 gcaacctaac ttctggtggc tgctggcaat tactgtctta tttggctgga tgagtctggt
    52561 cggcgtggtg cgggcggagt ttttacgtac ccgcaatttc gactacatcc gcgcggcaca
    52621 ggcgcttggc gtcagcgatc gcagtattat tctgcgtcat atgttgccga atgcaatggt
    52681 cgctaccctc acctttttac cgtttatttt atgtagttcg atcaccaccc tgacctcact
    52741 cgatttcctc ggcttcggtc tgccgctcgg ttcaccgtca ctcggtgaac tgctgttaca
    52801 agggaaaaat aaccttcagg ctccgtggct tgggatcacc gccttcttgt cggtggcgat
    52861 attattgtct ttgctgatct ttattggtga agccgtccgc gacgcatttg atcctaataa
    52921 ggcggtgtag catgacgcaa actctgttag cgattgaaga tttgtcggtg ggttttcgcc
    52981 atcagcaaac cgtacgtaca gtagtcaatg atgtttcact acagattgag gctggcgaaa
    53041 cgctggcgct ggtgggtgag tcaggttcag gcaaaagcgt taccgcgctg tcaattttac
    53101 gcctgctccc ttccccgccg gttgaatatc tctccggcga tattcgtttt catggcgaat
    53161 cgctgcttca tgccagcgac caaacgttgc gcggtgtacg cggtaataag atcgccatga
    53221 tttttcaaga gccgatggtg tcgttaaatc cattgcatac cctggaaaaa cagctttatg
    53281 aagtgctttc actccaccgc gggatgcgtc gggaagcggc tcgtggcgaa attcttaact
    53341 gccttgatcg cgttggtatc cgccaggcgg caaaacggct gacagattat ccgcatcagc
    53401 tctccggcgg cgaacgccag cgggtgatga ttgcgatggc gctgttaacg cgaccggaat
    53461 tattaattgc cgatgaaccg accaccgcac tggacgtctc tgtccaggcg cagattttac
    53521 agctgttgcg cgaactgcaa ggcgagttga atatgggcat gctgtttatt actcataacc
    53581 tcagcattgt cagaaaactg gcccaccgcg tggcggtaat gcaaaacggt cgctgtgtcg
    53641 agcaaaatta cgccgctacg ctatttgcct cacccactca tccttacaca caaaagctac
    53701 tcaacagtga accgtcaggc gatccagtgc cgttgccaga acctgcctca acgttgctgg
    53761 atgttgaaca gcttcaggtt gccttcccca ttcgcaaagg gattttgaag cgcattgtgg
    53821 atcataatgt ggtggtgaaa aacatcagtt ttacgctacg agcgggtgaa acactgggtt
    53881 tagtgggcga gtccggttcc gggaaaagca cgacgggact ggcgctgctg cgactgatta
    53941 attctcaggg cagcatcatc tttgacggtc agccactgca aaatttaaat cgccgccagc
    54001 tgttacctat tcgtcatcgc attcaggtgg tatttcagga tccaaactcc tcgctcaacc
    54061 cacgactcaa cgttttgcag attattgagg aaggcttacg ggttcaccag ccgacgcttt
    54121 ctgccgcaca acgcgaacaa caagtgatag acgtgatgca tgaagtggga ttagatcctg
    54181 aaacacgcca ccgttatccg gcggagttct ctggtggtca gcgacaacgt attgcgattg
    54241 ccagggcatt aattcttaag ccctcgctga tcatacttga tgaaccgaca tcatcactcg
    54301 acaaaacggt acaggcgcaa atattgacgc tattgaaatc attgcaacaa aagcatcaac
    54361 tggcctattt gtttatcagc cacgatttgc acgttgtccg cgcgttatgt catcaggtta
    54421 tcgtactgcg acaaggggaa gtagtggaac aaggaccgtg cgcgcgcgtg tttgccacac
    54481 cgcagcagga gtatacgcgt cagctactgg cgttgagctg acgcttaaaa aggattgtag
    54541 tttgaaaagg gttcggcaat tgccacacca aaatttttca atcggcatgt tgcagcaaac
    54601 tcgtcctggc gatttacaaa caggcacggc tcaccttcac actccagcac cgaacatgga
    54661 acttcgatgt cgcctaacgc ctggcttagt ttatacatca ccgaccatgc attatcacca
    54721 tccgggctaa gcaatttaag cgccaccagg ctgttgtcac cgcacggtcc attttgcgga
    54781 atcggttcaa ccttcgaacc tgcgaaacca gcagaccagc gatagttctg cggcagtcga
    54841 tggacgattg agagttgtaa tgaagtcact tattttcccc ggaagcacat ttacttcaca
    54901 atttgtttac atcaatttta acacatcatc aacaatccgt ctttatacag atcgtaaaga
    54961 ttgatgcctc gcctgcgtgg cctctatggc tctgatttaa gtaatatctg cgggcgtggt
    55021 cacgtttgcg gggctatatg tacccgtttc tgcgatctaa ctcaaccttt ttaactacaa
    55081 tgatgtgact ttttacataa attgatttta cataaaataa acatatatcg ggggaatgat
    55141 agatttgtgg ttgacatgca tcaacaaagg aagcctttta gcttcctcgt tgtgcaatag
    55201 atcaccgttt tttcggccga ctggcgtaca gacagaagag aatggagctg gttgcgcaga
    55261 atgcaattga ccaaatcatc ggccatgcag agttaaaggt cgcaagagaa agcaatgcgc
    55321 caacaattgc ccctatgcca aaacggaagg ttcctgccag cgaagatgcc gttcccgcca
    55381 tatggggaaa ctcatcaaga atgaccgcca tcgcattgga tgacaccatc gacacgcagc
    55441 ccacaaacgc cgcaacgcca accaccagcg accaaaatcc cagacccagc agcgcactga
    55501 tgaccatcca cgctgccata ataaattgta tccacaaccc cgagcggaac atatttaacg
    55561 cgccaatacg gcggacgaag cggctattaa agatggtcat cacgaacagg aaaacaatgt
    55621 ttagtgcaaa gtaataacca aagttttccg gcgcgacgtg gttaatttca atgtagacaa
    55681 acggtccggc gcttaagaac gagaacatcc cggcaaagct gaaaccactg gccagcatat
    55741 aactcaggac acgcttatga cggaacagcg ccgcaaagtt accaatagtg gtacgaatgt
    55801 gaaaaggctg gcgacgctcc ggtggtaagg tttctttgat cagaaagaag atcattgccg
    55861 aagccagaat cgccgccacg gcgaggatcc agaagatgta atgccagctc agccacacca
    55921 gcacccagcc gccaactatc ggtgccatca gcggtgcaat ggtcgtcacc agcatgacaa
    55981 acgacatcat ccgcgagaac tcttctttcg ggtagatatc acgcatcaaa gcgttgataa
    56041 ccacgctggc cgcagccgcc gccagcccgt ggaagaaacg catcacaatc agctgatcga
    56101 tggtttgtgc caacgcacac gccaccgcgg cggcggcaaa caccagcgta ccgccaagaa
    56161 ccaccggctt acgcccgaag ctgtctgcca tcggcccgta gattaactgc cccaacgcaa
    56221 agcccagaat ataagtactg agggtcatct gcgtactgcc cgccggtacg ccaaactgcg
    56281 ctgaaattac cggtagcgcg ggcagataca tatcaatcga cagcggcatc aacatggcca
    56341 gcaggccaag gataaaaaca atagcaaacg acgaatgctg tcgggtggtc acaacgggct
    56401 cctgaaagtc attgaaaagt tagacgacgc tggcaatttc ttcttcagtt aacggacgat
    56461 attcaccggg ggctaaatca gcatccagcg taatgccgcc aatacgttca cgatgcagct
    56521 caaccacatg gttacccacg gcggcgaaca tgcgtttcac ctgatgataa cgcccttcgc
    56581 tgatggtcag acgaacctgc gttggggtaa tcacttcaag caccgctggc ttagtgagat
    56641 ctttttcgtt atgcagctgc acgcctttag caaattgctc tgccgtatcg tcagctacgg
    56701 gtgattccag cgtcaccaga taggtcttct cgcaatgatg gcgcggagaa gtaatgcggt
    56761 gcgaccactg accatcatct gtcattagta ccagaccggt ggtatcgata tccagccgcc
    56821 ctgcggcgtg cagtttccac gccactggtt catctagaaa atagagcacc gttgggtgat
    56881 cagggtcgtc cgtggagcaa acatagccct gaggcttatt gagcatgaag taacgtggac
    56941 cgtgttgctg cgccagcggg ttgccatcgt aagcgacatc atgttcagga agcagtttga
    57001 acgctgcatt acggacgatt tcgccatcga cggtgacacg attgccgcgg atttcacgcc
    57061 cggcaatagc acggctaacg ccgagttgct gtgcgataaa tttatcaagt cgcatgtgtg
    57121 tgattttgcc tgtaaaaacg gaggtcgggc attgacccga aaatctgaac tgttgtctgc
    57181 ccagtatagc ggtctaataa cttccctcaa gggaaaaaga accgtggcat actattagca
    57241 gaataatcta cctacgcgag accatgattt ttacactccg cccatatcag caagaagccg
    57301 tggatgccac gctcaaccat tttcgtcgtc ataaaacccc tgccgttatc gtgctgccca
    57361 ccggtgcagg taaaagcctg gtgatagcgg aacttgcgcg gctggctcgt ggtcgcgtgc
    57421 tggtgctggc acacgttaaa gaactggtgg cgcaaaacca tgcaaagtat caggcgctgg
    57481 ggctggaagc cgatattttt gccgccgggc taaagcgcaa agagagccac ggtaaagtgg
    57541 tatttggcag cgtgcagtcg gtcgcccgta atcttgatgc ctttcagggt gaattttcgc
    57601 tgttgattgt cgatgaatgt caccgtattg gtgacgatga agagagccag tatcagcaaa
    57661 tcctcactca cctgacaaaa gtgaatcccc acttacgcct gctggggctg actgccacgc
    57721 cttttcgact gggcaaaggc tggatctacc agttccatta tcacggcatg gtacgcggcg
    57781 atgagaaagc ccttttccgt gactgcattt atgaactgcc gctgcgttat atgattaaac
    57841 acggctatct gacgccgcca gaacgactgg atatgccagt agtgcaatac gatttcagcc
    57901 gcttgcaggc acagagtaac gggctattca gcgaagccga tctcaaccgc gagctaaaaa
    57961 aacaacaacg tattaccccg cacatcatca gccagattat ggagtttgct gcaacgcgca
    58021 aaggggtgat gattttcgcc gccaccgttg aacacgcaaa agagattgtg ggattactac
    58081 ccgccgaaga tgctgcactg attactggcg acacccccgg cgctgagcgc gatgtgttaa
    58141 ttgaagattt taaagcccag cgttttcgct atctggtcaa cgtcgcggta ctgaccaccg
    58201 gatttgacgc cccgcacgtc gatcttatcg ccattctgcg ccctaccgaa tcagtgagtc
    58261 tttaccaaca aattgtcggg cgcggtctgc gtctcgctcc tggcaagact gattgcttaa
    58321 ttcttgatta tgcgggtaat cctcacgatc tctacgcgcc ggaagttggt acaccgaaag
    58381 gcaaaagtga caacgttccg gtacaggttt tctgccctgc ctgcggtttt gccaacacct
    58441 tttgggggaa aacgaccgcc gacgggacat tgattgaaca ctttggtcgc cgctgtcagg
    58501 gatggtttga agatgacgac ggtcatcgcg aacagtgtga cttccgtttc cgttttaaaa
    58561 attgcccgca atgtaacgca gaaaatgata ttgccgctcg ccgctgccgc gaatgtgaca
    58621 cggtactggt tgatccggat gatatgttaa aagcggcgct acgactgaaa gatgcgctgg
    58681 tattacgctg tagcggcatg tctttgcagc atgggcacga cgagaaaggc gaatggttga
    58741 aaatcaccta ttacgatgaa gacggcgcgg atgtgagtga gcgtttccgt ctgcaaacgc
    58801 ccgcccagcg aactgccttc gagcagcttt ttatccgccc gcatacgcgc acaccgggca
    58861 tccctctgcg ctggatcacc gccgccgata tcctcgccca gcaagcctta ttgcgacacc
    58921 cggattttgt cgttgcccgc atgaaaggtc agtactggca agtgcgtgaa aaagtgttcg
    58981 attacgaagg tcgttttcgt cgggcgcacg aattacgcgg ttaatggtag ttttgattga
    59041 tgtataaggc gattgagtat agaatctcgc ccgcttttgc atacgcaaag cagatcactt
    59101 acctgttgct gggtcgcctg tagcaggatt aatttaagag agaaagaaat gtttactatc
    59161 aacgcagaag tacgtaaaga gcagggtaag ggtgcgagcc gccgcctgcg tgccgctaac
    59221 aagttcccgg caatcatcta cggtggcaaa gaagcaccgc tggctatcga gctggatcac
    59281 gacaaagtca tgaacattca agctaaagct gaattctaca gcgaagttct gaccatcgtt
    59341 gttgacggta aagaaatcaa agttaaagct caggacgtac agcgtcaccc gtacaaaccg
    59401 aagctgcagc acatcgactt cgttcgcgct taattgctga ataagttgta aaaaaccccg
    59461 ctccggcggg gttttttgta tctgcagatt atgcctgatt acgatattgc tattttttgc
    59521 aggccagata aggcgttcac gccgcatctg gcattcataa cacaaaaccg attaattgcc
    59581 gccggacgtc cggcgctgca attgatcgcg caaattcggc ggcgtgcctt taatagtcag
    59641 ggtatcggtc gccggatccc agaaaatccg ttcgcccaac agcatcgcat caaaattaat
    59701 agtcaagcca ccgccgctac cggcaaactt cgtcaactga cgcagtgtgc tacggtctgc
    59761 cgggaagctc tcttccagtt cgtagccttt ttcagccgca aactccgtga agctcacttc
    59821 gctgacgcct gccagctctt tcgacagcga ttccagttcg atctcttcgc ctgcctgcag
    59881 ctgctcatta cagtagctat aaacctgctg gcgcacgttc tgtcgctctg ctttatccag
    59941 ttgtgcttcg gcagtgaaat catcaaccgc ttgcaacagc ccacggtttt gcgctttcgc
    60001 gttcaatcct tcgctggccc cgaggaaatc cataaagaaa tccgccactt tgcgccctac
    60061 tcgccctttc aggaaagtga gatagcgggt cgactctgga ttggtttccc attcggttaa
    60121 atcgatacgc gcaacaatat ccgcatgatt gatatcaaga taatgggttg ggttgatgtc
    60181 gagattttcg ttaacgcgca tactgctcag gttgctcagg actgccacca gcagatactc
    60241 caccgccaga taacggtagt ggcaaaacag cacgaatcca ccgtcagcaa aaggatattt
    60301 cgctaattcg tcacgcaggc gaccggttgc cgcgcggcta aatgccagaa agtcctcttc
    60361 accctgacgc tgtaagcgca gcgtctgcgc cagttcactc tcttcgctaa acaagccgta
    60421 agctttattt ttggcgctat agacccgatg cagttctgct accatctcaa cgacggtttc
    60481 tgtcggttcc agcaatgaat cgcgcaacac cagctcaagg ttctgctcat cacgcttgat
    60541 aagctggtgc agagcaatct ggttgatatc cagactcatg ataaactctc ctttaagacc
    60601 gggcggtatt caaccaccgc cggttaagcg acgcaataaa agatggcggt cattttacag
    60661 aaggcggcgt aacgagaatg atttaacgca taaaaaagca gaaaaaaaac cgttgctacg
    60721 gtaatatgtt gccctttcat caactaactg atttcgattt atgccacaaa tttcccgcta
    60781 cagtgatgaa caggttgaac aattgctcgc agagctgctc aacgtactgg aaaaacataa
    60841 ggctccgacc gacctttccc tgatggtgtt aggcaacatg gtcactaacc ttatcaacac
    60901 cagcattgcc ccggcccaac gccaggcaat tgccaactct tttgcccgcg ccttacagtc
    60961 ctctatcaac gaagacaaag cgcactaagg gaaacagata acaggttatg gtaactcatc
    61021 gtcagcgcta ccgtgaaaaa gtctcccaga tggtcagttg ggggcactgg tttgcactgt
    61081 tcaatattct gctttcgctc gtcattggca gccgttacct gtttatcgcc gactggccga
    61141 ccacgcttgc tggtcgcatt tattcctacg taagcattat cggccatttc agcttcctgg
    61201 tgttcgccac ctacttgctg attctcttcc cgctgacctt tatcgtcggc tcccagaggc
    61261 tgatgaggtt tttgtccgtc attctggcaa cggcgggaat gacgctatta ctgatcgata
    61321 gcgaagtctt tactcgtttc catctccatc ttaatcccat cgtctggcaa ctggttatta
    61381 acccagacga aaatgagatg gcgcgcgact ggcagctgat gttcatcagc gtgccggtta
    61441 ttttattgct tgaactggtg tttgcgacgt ggagctggca aaagctgcgc agcctgacgc
    61501 gtcgtcggcg cttcgcgcgt ccgctggccg cattcttatt tatcgccttt atcgcctcgc
    61561 atgtggtgta tatctgggcc gatgccaact tctatcgccc tatcaccatg cagcgcgcta
    61621 acctgccgct ttcgtacccg atgacggcgc gacgttttct tgagaagcat ggcctgcttg
    61681 atgcgcagga gtatcaacgc cgtcttattg agcaaggtaa tccagacgcc gtttccgttc
    61741 agtatccgtt aagcgaactg cgctatcgcg atatgggcac cgggcagaat gtcttgttga
    61801 ttactgtcga tggcctgaac tactcacgct tcgagaagca gatgcctgcg ctggcaggtt
    61861 ttgctgagca aaatatttcg ttcacgcgcc atatgagctc cggcaacact acagacaacg
    61921 gcatctttgg cctgttctat ggcatctcgc cgagctatat ggacggcatt ctgtcgaccc
    61981 gtacgcctgc ggcattaatt actgcgctta atcagcaagg ctatcagctg gggttattct
    62041 catcagatgg ctttaccagc ccgctgtatc gccaggcatt gttgtcagat ttctcgatgc
    62101 cgagcgtacg cacccaatcc gacgagcaga ccgccacgca gtggatcaac tggctgggac
    62161 gctacgcaca agaagataac cgctggttct cgtgggtctc tttcaatggt actaacattg
    62221 acgacagcaa tcagcaggca tttgcacgga aatatagccg ggcggcaggc aatgtcgatg
    62281 accagatcaa ccgcgtgctc aatgcactgc gtgattctgg caaactggac aatacggtgg
    62341 tgattatcac tgccggtcgg ggtattccac tgagcgaaga ggaagaaacc tttgactggt
    62401 cccacggtca tctgcaggtg ccattagtga ttcactggcc aggcacgccg gcgcagcgta
    62461 ttaatgcgct gactgatcat accgatctga tgacgacgct gatgcaacgc ctgctacatg
    62521 tcagcacacc tgccagcgaa tattcgcaag gtcaggattt gttcaaccct caacgccgtc
    62581 attactgggt taccgcagcg gataacgata cgctggcaat taccaccccg aaaaagacgc
    62641 tggtgctgaa caataacggt aaataccgca cttacaactt acgtggtgaa agagtgaaag
    62701 atgaaaaacc acagttaagt ttgttattgc aagtactgac agacgagaag cgttttatcg
    62761 ctaactgatt aattataaat cagttagcga aatatcttac ttgcaatcgg tgtggaaaac
    62821 ggtagtatta acaaccacga gtcggcacgt agcgcagcct ggtagcgcac cgtcatgggg
    62881 tgtcgggggt cggaggttca aatcctctcg tgccgaccaa aaatcccaaa aaaaccaacc
    62941 gtaaaggttg gtttttttat atctgcattt aattcgataa acagaccgcg acacatcagg
    63001 gcctgtttat tgtctgttat cagaacgtcc agaccacacc cgcctgggta ttccacggcg
    63061 attctacccc tgcgccattg ccatagctga ctgacaaatg accgcttaac gtcggggtaa
    63121 acgatgacct tatcccagcc tgataaacgc cacgggttcc agccacatca ttattaaatt
    63181 tgccatcgtc attaactttc acctggttag aatcggcgta ttcctgacgc acggcggctt
    63241 tcagccaggg ttccagcgtc gtaccgtttt gcaggtccat gtgatagctt accgccgttc
    63301 ccgcttcagc gcgtaatatc cgggtatttc ccacatccgc gcgcatgccg tttgataacg
    63361 tgtagtcctg accatctgtg gtaaagccgg taaaggccag atagggtcta acactccaca
    63421 atccgtcaac ccaacggaac ccgctctcga catgagcacc cgcgccgtta ctattgtaat
    63481 cgccaaacgc tgttgcccca ttactcatct tgccatggat ggtgttggca aaacggtcaa
    63541 ctttcaccac tccatcaaca taggcaccgt tctgatgctc ccaaccggca taagccccca
    63601 gggtatagct atcgacattg cctttgccgc cgcgatcaaa accaatatca gaatgagagt
    63661 aaccaaagaa caagccgcga attgtgctgc tttcttcacg ggagaaacgg ctatcgatac
    63721 cgagcgtcag gcccgtcaat gtttgctcaa aaccagctcc cgcatcagtg gtcacgttgt
    63781 tgcgggtgtt aattgccgaa ctccacatcg ccgtatcgta actaacgcct tttacgctac
    63841 caagacgctc acgcacggtg tccagttctg catcaaatac cagcggttgt gcggccgcca
    63901 tattcagcac atcagtggtt gaaggggtaa tttgcgcgcg attctctgcc agactccagc
    63961 tatggttacc attatccagc aaggtatatt catacgtacc gatatcaaca acgcctccgg
    64021 cattgcccaa cgtaaatgca gcatcaccgc cgcccgttgt taccagtgta aggctatctc
    64081 ctgctgccgg gctggcaccg gtgtccgtca cgaatatttt gaaatcacct gttgcctgac
    64141 cggtgacgtt gagctgatcg ctctgatgat tcgccatatc ggtacgcata taaaaattgc
    64201 cgtttcccga cagggtattg gtggtcaacg tagtgaaatt ttccgcactt gccgtcacag
    64261 atgaacgagt cactgactca tccatcataa tcacatttgc gccagacatc gcaaggttgg
    64321 ctaagttaag gcgggtgttt tggttcgtcg tagtgatatc ggcaatgatg gtccatgcgc
    64381 tattttcgag cgaaacatcc gctttgctgg tatccacggc accatgcgtt cttaaagaag
    64441 cgccctcaga aaccactacc ttacccacca acattgcggg tgcataattt atttgcggct
    64501 tcataacctc aagaatgcca tcatttcctc tgactgacac gttaaccatt gtgccagccc
    64561 agacgttagc gcggccattg ttgataacca tattttcaga gatggcttta gaggaatacg
    64621 ttatacttcc attctgatct gatcgaccga gatcatatac cgcatttgcc tgcactctcg
    64681 taccagtatc ctttcccagc gattgcatag tggcatgctc atcgaggata gtgtcaatgg
    64741 cctgcgtgtc ctccataaca ataagcccgg aaccatcatc cagttcataa ttttttgaca
    64801 caccatcttt tatactaaat tggcctttac tgtttgtacc actcacttcc agcgcattcg
    64861 ttgagacaat taattttccg cctgcttttt tatcaacgcc agttgcagtc ccgccatcca
    64921 taacctccag taagccgcca ctatctacag tagtattata agcgaagtca ttttcttcaa
    64981 ctcgcaaact tccgccgttt tccaacagca tattgttagc aataccattc ttgatttcga
    65041 actgaccgag acggtttgtt ccgaatacct ccatgacccg cgtgcttgct ttaatagcac
    65101 cgcctgcttt ctgatctacc gcaatcgcta atcccccctc tttaacctcc agtaatcctc
    65161 ccttttcgat agcggcaagg acggtaatcc catctttttc aacgattaag cgcccgcctt
    65221 taatcgtggg atcattcgct gcgccataaa cctgtagcac accattattg agcgtagtct
    65281 ggtttgccgc gccacccatt tgaacaattt gataaccatc atttaatact gagtattctg
    65341 cggtgccatt catttcaatg tactgatacc cgccgttaat ttcagtatta ctacttatac
    65401 caaattcttt aatatgctgt tctccgccag gattgatttt cgtattattg gcggtcccat
    65461 aaaccagctg gatatctttt tccaggacaa caccatcaac tgtctcacca tactcaactg
    65521 ttgatgccaa tgctatgggt gaatataacg ctgtagctac cagaacagat attgcggaac
    65581 gacaaagaga aacagaacca gattgatgca ttgagctttc atcctatgaa attaattgca
    65641 gttaaaagca ttgggtacag aaaataccca tagctccata cccggagtca gtttttaaaa
    65701 actgtttaaa gaaatgcaca agtattgtga ttgatttttt agttgttttt cttgatgaga
    65761 agctgatgca aaattccgtc tttataatga aaatgatgcc aaagcgaacg acaaggttgt
    65821 agttttcgct acatgtccat acataaaatg gggtaacatt cacgcgcctg gtagcgttac
    65881 caacgctacg ctcaaacata atgattctaa taaaacctca ggagactact atgcctgaag
    65941 caacgccttt tcaggtgatg attgtggatg atcatccact tatgcgacgc ggtgttcgtc
    66001 agttactgga gcttgatcct ggctttgaag tggtcgccga agcgggcgac ggcgcgagtg
    66061 ctatcgatct ggcgaataga ctggatatcg acgtgatctt gctggatctc aatatgaaag
    66121 gtatgagtgg cctggatacc ctcaatgcct tgcgcaggga tggcgttacc gcgcaaatta
    66181 ttatcctgac cgtatcagat gcctccagcg atgtctttgc actgatagac gcaggcgcag
    66241 acggttatct gttgaaagac agcgacccgg aagtgttgct ggaagcgatt cgtgccggag
    66301 cgaaaggcag caaagtcttt agcgaacgcg tcaatcagta cttacatgaa cgtgaaatgt
    66361 ttggcgcgga agaagatccc ttcagcgtgc tgacggagcg cgagctggat gttctgcacg
    66421 agctggcaca ggggctgtca aataaacaga ttgcctcggt gttgaatatt tccgagcaga
    66481 cagtaaaagt acatattcgc aatctactgc gcaaactcaa tgtccgctca cgcgtggcag
    66541 ccaccattct gttcctgcaa caacgcgggg cacaataaaa atagcccgat ggcatcacca
    66601 tcgggcctct ttttatttac tctcctgcgg cgacaaatgt tgcatcgcct gcgcgatact
    66661 acgttcaatc accgcacggc gagtatcgtt ggcaggtaag agtttcaaca tcatctccca
    66721 cgcggcaacg gcttcgccaa atcgctgctg ctcaaaggca ttaaacgcat acatgcttaa
    66781 cacacgaata ttgctatggt ccgttctcac caactggcgc agcagttcgc cgccgaggcg
    66841 gttgtcgttg ggatcagatg aacgtgtcaa cgcttcagca taacccagtg tggcatcgct
    66901 gtttttcgga tcgaggcgat acgcagtagc gtatgcatcg gtggcgatac tggcgttacc
    66961 cagcgccatg ccaacgcgac ccaacataat ccaaccttct atatctcccg gatttttttg
    67021 cagttgagta cgcatcccca gcgcaaggcg cgacatctct tcttcgttga gcggatcggc
    67081 tttcggatcc agcgccctgt ccagcaacgc cggagcctgt gccgtggcct gctgccagat
    67141 tttcacctgc tgataattgc cagtctggta gtagctgacg ccagccacaa ttaacgccac
    67201 cacaataccc ggcagataaa caacatatcc ggcacgctta ccttccggca cgctttgttc
    67261 aggaaacgct tccggtacca cgcgtacccg acgccgcgaa cgggcgtata tgacccaacc
    67321 gccaatacca atagccacta ccggaagcac ccacagcagc acggtcagcg gcgttaacgg
    67381 cggatcgtaa gtgacgaagt tgccgtaacg cgccaccata taatcgacaa tctctttctt
    67441 acttttgcct tcctgcatca gttcatacac tttctggcgc aggtcggtgg caatcatcga
    67501 gttggaatcg gcaatgctgt tattctggca tttcgggcag cgcagttctt cagtgagctg
    67561 acggaactgt tgttcctgtg cttcatcttt aaactgcaac acgtcgatgg tcgccagcgc
    67621 tgagccggag atcatcagca tcagcacgcc caataaaaac ctcattgtgc ggcctcctta
    67681 ctgtatttct cccacagcgg cttgatctct tcttcccaga cgcgaggatt gagatcgccc
    67741 gcatggcgat agcgaatgat gccgttgccg tcaataagaa acgtttctgg cgcgccatag
    67801 acaccgagat ccagccctaa catgccatcg ccatcaaaaa ggcttagcgc gtaaggattg
    67861 cccagctctt tcagccagct gattgccttc tggcgatcgt ctttatagtt catgccgacc
    67921 acgcggatgc cctgcgcaga aagctgattc agatattgat gttccgcacg gcaggtcgga
    67981 caccaggtcg cccagacgtt aagcagcact ggtttgccct gagtcagcac atccgcctga
    68041 taaaactgcc ccggattgtc cagtgattcg agacgaaact tcggcacagg cttgccaatg
    68101 agcgccgatt ccagattggt cggatcatcc ccttcggcat tacgcgccag ctgccacagc
    68161 agcgccgcgg caatcgccag gaagataatc aacggaatta acaatacttt gcgcttcata
    68221 cagcctccgg cgcagttttt tgcgggctca cgcgcttacg atagcgagga tcaaacagac
    68281 acagcagtcc gcccaacgcc atcatcagcc cgcccgccca aatccagcga acaaatggtt
    68341 tgtagtaaag acgcacagcc cacgcgccgt tttccagctc ttcaccgagc gcggcgtaca
    68401 ggtcacgcgt gatgccgccg tcaattgccg cttcggtcat catcgacccg gcagtgttgt
    68461 aataacgttt ttccgcatac agcaccgttt ccggtttacc ctctcgcgtt acgccgatag
    68521 tcgccacacc gccacgccag ttcgggccag tcacctcttt gacatcacgg aaggtgaagc
    68581 gatattcatg aatatcgacg ctatcgccgg acttcatgcg cacatcacgc tcaacgctat
    68641 agttctggct aaaggcaatg ccaacaattg tcactgccag ccccaggtga gccgccacca
    68701 tcccccaata actgaaggtg gttttcgtgc cgcgtgaaat acgtagcgca gcttccgcaa
    68761 ttgccagcac cgcaatccag caggccattg ccaggccgag caccgtcatc gccacaactt
    68821 tgctttcgaa cagccacggc aacagcagag acagcaccag cgtagagatg aaggcgataa
    68881 tcaataaatt gcggatttta cgtgggcgat cgcgccccca gcgcaccaga ggaccgacac
    68941 ccagcagtag cgcaaacggc accatcagcc aggtaaacat ggtgttgaag aacggttcgc
    69001 caatcgaaat actgcccagt cccagttgct tgtgcaccag cggcagcagc gtccccagca
    69061 acaccaccag catcgcggcg accagcaaaa cgttgttcgc taacagcaaa gattcccgcg
    69121 accacagcgc attgtttacg cgtgagcgaa ctttgtgtcc acgcgcggca aacagcagca
    69181 gcgaaccgcc aatcaccagc accataaagg cgaggataaa cataccgcgc gccggatcag
    69241 acgcaaacgc gtgtaccgat accagcacgc cggaacgcac gaggaaagtc cccaacagac
    69301 acaacgagaa ggcactgatt gccagcagta atgtccacgc tttgaagctg gcgcgttgtt
    69361 cagtgaccgc cagtgagtgc atcagcgcag tccccaccag ccacggcata aacgaggcgt
    69421 tttctaccgg atcccagaac caccagccac cccagccgag ttcgtaatag gcccatgcgg
    69481 aaccgagcac gatgccgagc gtcaggaaga tccacgctgc cagcgtccac ggacgagtaa
    69541 aacgcgcata agtgctgtcc agacgcccgc tcagcaaaga agcaatggca aaagcaaacg
    69601 ccaccgagaa acccacgtac cccatataaa gcagaggcgg atggaagatc agccccggat
    69661 cctgtaacag cgggttaaga tcgcgccctt caatcgggaa gttcggcagc gtgcgtgaga
    69721 atgggttaga ggtaaagaga atgaacagca aaaagccgac actgaccatc cccattatcg
    69781 ccagtacgcg ggccacaata tccagcggaa tacgctgact aaaaatcgcc accgcaaagg
    69841 tccagccgct catcagcagc acccacagca gcagtgagcc ttcatgtgcg ccccaggtag
    69901 ccgccacgcg ataccacacc ggaagctggg tattggagtt gctggcaaca taggtgacgg
    69961 taaagtcgtt gaccacgaaa gcattgacca gcaccagaaa tgcgccagcc acagacataa
    70021 acagcagcca ggcaaacaag cgggaagacg ccatcatgcg cgcatctccg cgcgccacgc
    70081 cccatagcgg atacacggac agcagcagcg caattcccag cgccaggcac agcagcccgt
    70141 taccaatttc cggcatcatg atgctgggtc cttataaaca ctcgccgggc gacggtggtt
    70201 agcttccatc gctttctcaa cttctggcgg cgtgtagttt tcatcatgtt tcgccagcac
    70261 ttcttttgcg aggatatgat tgcctttttc cagctcgccc tgcaccacaa cgccctgccc
    70321 ttcacggaac agatccggca aaatgccttc gtaagagaca tccactgagc cttcagcgtc
    70381 gtaaatggtg aaggtcactt tcagcgaatt gggatcgcgc tgcacactac ccggcatcac
    70441 catcccgcca acgcgcagac gctgaccgac ttccggcatc tgctgagttt cacgcttgcc
    70501 gtagagaatt tcccccggcg tataaaagag atcgatattc gagcgcagcg catatagcac
    70561 cagaccgata gtcagcgcca gccctgccaa cacggcacag gcaatccaca agcggttttt
    70621 acggcgaata ttcatgcagc ctcctgctgt tgcgcagcac gtaaacgcgc ctcacgcgcc
    70681 cgctgttgcg ccacaccacg cagaattgcg cgatgctgca tcaccgagtg cacgaccaaa
    70741 accaccagcg gaataacggt catcaccacc gccagccaga caaaaaaggc gtaaccgccc
    70801 attgcgaaaa attcattcca ggaagcaaat gcaggggtca tttacggcct cttttcagta
    70861 tcagttcact cacccacgga cggcgttttt ccatcagcaa aatcaaatta cgcatccgca
    70921 tcagcgtcag cgtggcagag aggagcagga agccaaaaat cgaccagcgc agcggcgaac
    70981 gcatcgccgg atcgatactt tgctgcatcc gcgttgatcc ctgatgcagg gtgttccacc
    71041 actccacgga gtaatgaata atcggcagat tcaccacgcc aatcagcacc aggatacctg
    71101 ccgcacggcc cgccagacgg cggtcgtcga aggcgtgcca cagggcaatc acacccacat
    71161 acaaaaacag cagcaccagt tcagaagtca gacgtgcatc ccatacccac caggtgcccc
    71221 acatcggttt tccccatgca gagccggtaa ccagggcaat aaaggtaaac acggcaccaa
    71281 tgggggccat cgccgccacc gccaggttgg ccattttcat ctgccagaca aggccaataa
    71341 acgccgccac cgccattgat gcataaatgc ccatcgacca gatcgccgca ggcacatgca
    71401 ggtagataat gcggtagcta tttccctgct gataatcagc cggagcaaag ccgaatcccc
    71461 agatccagcc gacggtaagc acgactacac tggcaattgc cagccacggt ataaaccagc
    71521 cacagatttg atacagccgt ggtgggatcg ccagttgatg cagtgttttc cacatagttt
    71581 cgataccaga ctcgaacaaa aatcagtaat ccagcgttat tgaatgctga ttcgtaacgc
    71641 tgccgccgtc gcaaaaggac ttaatgtcgc ggtgcctgcc agcaacgcgc ctaaaattgc
    71701 cagatacccg tcaacgggca aatgcataga agccgcgtcc atcgcggcgg tggcaaagat
    71761 gagtaatggg atagtcagcg gtaacaccag tatgctgagc agcacaccac cgcgcttaag
    71821 tcccactgtc agcgccacgc ccggtgcacc gagaaagcca agcgtaggcg ttcccagcag
    71881 tagcgttaac gccatcactt gccagccata aacatccatt cccagtagca ttgctaccag
    71941 tggcgaaagg atgagtaacg gcagaccggt taccatccag tgcgccatca ccttcgccag
    72001 cacaacggcg ggcaagggta acggcaacaa catcaattgt tcaagactgc cgtcctgcaa
    72061 atcgtcacgg aacagtcgtt ccagcgccag caaggatgaa agcagcgcgg caacccagat
    72121 aatgcccggt gcaatacgtg ccagcagttg cggctccgga ccgatactga gcggaaaaag
    72181 ggtaattaca atcaggaaga accacagcgg gttggcgatt tcagcgctat ggcgaaacgc
    72241 tacgcgcagc tcaagacgga aaatgcgcca gaacatcata cggccctcgt ttgcgtcagt
    72301 gaaatgcggc gaattttact ttcagcaacg ttgagcggct ggtgggtagt gagaatcaca
    72361 atccccccct gctctgtatg ctgcgccata cgctgggtca gacgatcgac gccgttaacg
    72421 tcaatcgcgg taaaaggctc gtcgaggatc cataacgtgg cacgggtcag ccacagacgc
    72481 gctaaagcga cgcggcgttg ttgcccggca gaaagttgat ttaccggaat atcttcgaat
    72541 ccggcaagcc cggcctgcgc cagggcttcc agacattgtg cggtatcgcc atcgcgatga
    72601 taaaagtgca gattttctaa cgccgtcagc cgggttttga tccccggctg atggcctatc
    72661 cataacaggt tttgatggta gctgtcgcgt acctgatgca agggctgccc ttgccagaga
    72721 acctcgcctg cgtcagggcg agacaacccc gtcagcaaac ggagaagcgt tgtcttcccc
    72781 gcgccgttgc taccggtgat ttgtacccac tctcctgcgt tcagcgtaaa tgacaagcca
    72841 ctaaataagg ttcgttcatc ccgctcacaa agtaactctc tgacttcaag catacccacg
    72901 caataaccct gttaaaaacc tggctcgact tcacgcatat cgggcagctt gtgcgctatc
    72961 cctttatggc aatcaataca ggtttgccca tctttcaccg cctggtcatg catcttcgcg
    73021 gcaaccgatt tctgggcggt tgtatccata tactcgaagt tgtgacagtt acggcactcc
    73081 tgcgagttat tgtccttcat gcgccgccac tcattctgtg ccatcgtcag acgatgagct
    73141 tcaaatttct gcggcgtgtc aataacgcca aaaattttac catacagctc tttacttgct
    73201 ttgagcttgc gtatcatctt cggcacaaac tcgtgcggaa cgtgacaatc cggacaggtc
    73261 gcacggacgc cgctacggtt gttgtagtgc acggaatcca tgtattcctg atacaccgtg
    73321 ttacgcattt cgtggcagct aatgcagaac tcttcggtat tggctttttc catcccagtg
    73381 ttaaagccgc cccagaagac gatgccgccg acaaaaccga tcaacagcag cgtccccagc
    73441 gccagacggc tgggggtacg ccaccatttc cacaggcgct taatcagacc aggcttacgg
    73501 tcagaatttc ccataataac ctcttatttc ccgtaacctt ttgatggggt aaaggtattc
    73561 cccacgattg gcgcggtatc ggcctgcggt acgtgacatt gcagacagaa ataacgacgc
    73621 ggagccactt ccgcgcccac tttgccgtcg ctgtccataa agtgagtagg actgatacgc
    73681 ggcgcgccag tggtgcgata gctttcgaca ccgtggcact gcaagcagcg gttggtattg
    73741 gtcgttacct gataaccttc aacgctatgc gggatcattg gcggctgatt cacatagttc
    73801 agcggcatcc gatcctgctc ttttggcatc cgaatggccc cttcctgcgt cccggatact
    73861 tccggcgact ggctaaaatc gacgccatta gccgcccaaa ccgcgccgct taccaccagg
    73921 gccagcatcg ccgtccattg acacagcgct ttcttcaggt catggctttt catgatttcg
    73981 ctcccgaact ccatcgtgta gttattgtaa atacatcctc agaacagaca tccacgcagc
    74041 gaccgcacgt catgcaatcg cggctggtga cctgcaccgg gctttgttca tccagcaccg
    74101 gggcacgtag cacatgcggt tccgggcaaa catgaaaaca atccatacag cggttacatt
    74161 tctgccgatc gctggccgca acggtaatca cacctttgct acccagcacg ccatacagcg
    74221 cgccaaccgg acaaatgtgc ccgcaccagc cgtgttcaac gaccagtaga tcaaataaaa
    74281 acagcgcgag aataagcagc gcgccgctgc cgaagcccat taccaggcta cgccccatca
    74341 gggaaacggg gttgatccat tcccagatga gcgtgccagt taaggctgat cccaccagaa
    74401 tgaccaccaa cagcacgtag cgaatgtggc gagggatcgt cgcagactga ttgaggtcaa
    74461 agcgcctgcg taaccagttc gctaagtcgg taatcgggtt cagcgggcag acccagctgc
    74521 aaaataatcg ctttcctgcc agggcgtaga gcacggtgat aatcaccgca ccggtcaacg
    74581 ccaccgtggc aggcagatga ccactggcaa gactttgcag cgtcatcagc ggatcggtca
    74641 gcggaacggt gtcgaataac aggctactgc tatagttgcc gtgcaagatc cacacaccaa
    74701 accacggacc gctcaaaaac atccccagca cgaagaactg acaaaggcga cgtaacacca
    74761 gccaacggtg actgcgccac cagccttttt tctccagcgc ctcgcgcccg gcgtcacgtt
    74821 tacgatttgc cattgttccc ctccagccag ccgaagcggt aatggtgacc taactcccct
    74881 ttcgccagtg acagcggtaa caccttgatt gccggttgtt ccagcacgca tactttttcg
    74941 cacttaccgc aaccggtaca ggcgtcgctg tgaaccgtcg gcagaaagcg ggcgtgctta
    75001 ccggtacgcg tgttgcgctc cagctccagg gtgatggcct catcaatttt cgggcattcg
    75061 cgataacaaa catcgcagcg cagcccctga aagttgagac agttttcctg gtccaccagt
    75121 accaccagcc ccatccgcgc gtcgtcgatc gattcaatct cacgatccag cgcaccgctc
    75181 gggcacactt tggcgcacgg aatgtcctca cacatttcgc aaggaatatc ccgtgcgaca
    75241 aaatatggcg tgcccgccga caggccagag gccagcgtcg ccagttttaa ggtgtcgtaa
    75301 gggcaagcct gaacacactg accgcaacgc acacaggcac tggcaaaggc gttctcgttt
    75361 atggctccgg gcgggcgcaa ccgcacgcca gatgcgcgtg cggtttgctg ttgtaacccc
    75421 agcgccacac ccacggcagc cagcccgcct gctgtgcgaa cgacatcgcg cagaaagcgg
    75481 cggcgaccat tttgaggttt cgctgaccgg gacataatgg cttacacctt ctccagtttg
    75541 accgcgcact tcttgaagtc cgtctctttc gagagcggat cggtcgcgtc cagcgttagt
    75601 ttgttaacca gctgtgcggc gtcgaagaac ggcatgtaca ccaggccctg cggcggacgg
    75661 ttacgaccgc gcgtttcgac aatcgagatc acttcgccac ggcgagaaac cactttcact
    75721 ttgtcgccac ggcgcagatc gcgcgctttc gcatccagcg ggtgaataaa caggaccgct
    75781 tccgggaagg cacggtgcag ttccggcaca cggcgagtca tactgccggt gtgccagtgc
    75841 tccaggacgc gtccggtaga gagccacagg tcgtactctt catccggtgc ttccgccgcc
    75901 ggttcgaacg gcagcgcgaa gatcaccgct ttaccatccg gtttaccgta gaacttatag
    75961 ccttcgcccg ctttcacgta cgggtcgtta ccttcgctgt aacgccactg cgtttcttta
    76021 ccgttcacca ccggccagcg cagaccgcgc gctttgtggt agtcatcgaa cggcgcgaga
    76081 tcgtgaccgt gaccgcgacc aaaccatgcg tactcttcga acagcccttt ttgcagatag
    76141 aagcccagct cgcgggattc atcgttcagc tgatcttccg ccagttcgga taccgggaat
    76201 ttgctcactt cgggggtggc atacagaact tcgtacagcg ttttgccacg cagttccggt
    76261 ttcttcgcca gcagctcttc cggccatact tcttcagttt tgaagcggcg ggagaactgg
    76321 actaactgcc agaggtccga tttcgcttcg cctggcgcct gtacctgctg acgccagaac
    76381 tgagtacggc gttcggcgtt accgtaagcc ccctcttttt ctacccacat tgcggtcggc
    76441 aggatcaagt cggcggccag cgcactgact gtcggatacg gatcggagac gatgatgaag
    76501 ttgcgcggat cgcgccagcc cggcatacgc tcttcattaa tgttcggccc ggcctgcatg
    76561 ttgttggtac acatggtcca gtaaacattc agcttgccgt ctttcagcgc acggtcttgt
    76621 gctaccgcat gcagaccgat tttcgccgga atggtgccgc tcgggatatt ccacttcttc
    76681 tcgcagatat cacgatgttt ctcgttagtt accaccatgt ccgcaggcag acggtgagca
    76741 aaggtgccca cttcacgcgc agtaccacac gcggaaggct gcccggtcag ggagaacgga
    76801 ccgcaacccg gctgggaaat tttgccggtc agcaggtgca ggttgtagac caggttgttg
    76861 gcccacacgc cacgagtatg ctggttgaag cccatcgtcc agtaggagat gactttcttg
    76921 ttcggatcgg catacagctg cgccagttgt tccagctgat cttttggtac gccagtcatt
    76981 tcagcggttt tttccagcgt atactcagca acgaaggctt tgtaatcttc aaagctcatc
    77041 ggttcggagg cgtcagaacc cggattcttc gctgcttttt ccagcggatg ggtcggacgt
    77101 aaaccgtagc cgatgtccgt cgcccctttg cgcaggttaa cgtgcttgct gaagaagtcc
    77161 tgatttatcg cattgttttg aatgatatag ttggcgatgt agttcaggat caccaggtca
    77221 gattgcggcg taaagatgat gccgttatcc gccagctcga agctacgatg ctggtaggtt
    77281 gaaagcaccg ccacggtgac gtcctggtta gagagacgac ggttagtgat gcgtgaccag
    77341 aggatcgggt gcatctccgc catgtttgcg ccccacagca caaacgcgtc agcctgctcg
    77401 atgtcgtcat agcagcccat cggctcatcc ataccaaagg ttcgcataaa gccaactact
    77461 gccgacgcca tacagtgacg cgcattcggg tcgatgttgt tcgaacggaa gcccgcttta
    77521 aacagcttgg acgcggcata accttcccag atagtccact gaccagaacc gaacataccg
    77581 atcgattccg gccctttttc tttcagggcg gttttgaatt tctcttccat cacatcgaag
    77641 gcctgatccc aggtgattgg ggtaaattcg ccttctttgt catatttacc gtttttcata
    77701 cgcagcagcg gctgcgtcaa acggtcttta ccgtacatga tcttgggcag gaaatagccc
    77761 ttaatgcagt tcaggccacg gttaaccggt gcgtccgggt cgccctgaca ggccaccaca
    77821 cgcccctgct gcgttccgac cagaacgccg caaccagtac cgcagaaacg gcacggcgct
    77881 ttatcccatt taatggcttc ctgctgacca acaacggcgc gggcaacgcc cggcacgctg
    77941 agaccggcag ccgccgcagc ggccgcaacg gcgttagctt tcataaagct acgacgactg
    78001 agtttcatgg tgtttcctca ccttgctctt cctgctggtg ataaaccagc gacaccgcca
    78061 gcacgccctc tacgttgcgt actgactcaa tggtttggat cagcgtttcg ctgtcttctg
    78121 cttccaccac cacaatcaac tgaccgctcg gcgcgtcgct gacagcaact tcacagccgg
    78181 gaaaggcgtt cagttgggtg ctgatgtctg aaattcgttc gcttttggcc tgcacaacca
    78241 ggctgcaaac ttgccagtta gtgtgcatgg agatactccg cagttatggc tgataccgga
    78301 cagctggctg cacatgctcc acatccgttg caaagttggc tattaagttg cggctggtag
    78361 atcccggaga gcgtcgggcg aaagataatt gccattggct cacagctatc ctgacagcgg
    78421 cggcattcaa ctgactgata cgccaggcac gcgtccccaa tggtgaactg caaatcccag
    78481 gccctggtgt ggcgcggaga aaatagcgat tcggggcaag cctgcgcgca ggcgtaacag
    78541 aagctgcact cgttattttt gaaattaacg ctcggatagc cgcccgcgcc gcgttgcaga
    78601 atgttgtttt cacaagcatt aatacaggcg tcgcagcggg tgcaatgggt cagaaaatga
    78661 gattcatcac cggaccaggg cggacggata ccgttactgg ctttgcgcca gcgaccagtg
    78721 agtatgcccc gacgggatgc atcaatcttc acattgacct tccatcatta acgcgctctg
    78781 aaaattgaga gcgaccaaat aaaccgcata attaataagc catttttata gccgctaaga
    78841 tattaaagga tgtgtcaaag atgcataccc cgatcgaggt aaaacctgta gcaggatcaa
    78901 aagagtggcg ggaagcgtgg caaaaacggg cttttgctca catttcaaat ggttataaat
    78961 atatttatat agcgattaat tcaccagaga tatttctgct ggtttgctct ctcatcagaa
    79021 tttaacact
//
LOCUS       DAZFAB010000011        71228 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZFAB010000011 DAZFAB010000000
VERSION     DAZFAB010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 71228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71228
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.011"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            60..584
                     /gene="lpfA1"
                     /locus_tag="ACHJWA_000893"
     CDS             60..584
                     /gene="lpfA1"
                     /locus_tag="ACHJWA_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA1"
                     /protein_id="HFO4307544.1"
                     /translation="MKKVVFALTALALTSGTVFAAESGDGTVKFTGEIVDSPCVLSVD
                     SQNQEVVLGQVQKSVFAAVGDKSPAKPFEIKLEDCDTTTMKKANVSFSGVGDADKSDL
                     ISVSTEAGAAKGVGIGIYDNSNTLVALNGGKASVDLSKGQTVLYFTANYVSTLATVTT
                     GYGNAQVDFNLSYE"
     gene            649..1335
                     /gene="lpfB"
                     /locus_tag="ACHJWA_000894"
     CDS             649..1335
                     /gene="lpfB"
                     /locus_tag="ACHJWA_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312457.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial biogenesis chaperone LpfB"
                     /protein_id="HFO4307545.1"
                     /translation="MKSKFVALALSLFLSQSVLAGGVGLSSTRVIYDGSKKEASLTVQ
                     NKSKTEEFLIQSWVDDAAGSKKTPFIITPPLFKLDPEKNNILRIVNITPGLPQDRESV
                     YWVNVKAIPSKSDDSENKNVLQIAVRTRIKLFYRPAGLKGDVKTAPNELRFTRNGNQL
                     RVDNPTVFNITFNQFFANDKEIEKAGMVPAKGALNITLPAGVGSVSKIKYNTINDFGS
                     SAEMITKNVD"
     gene            1364..3937
                     /gene="lpfC"
                     /locus_tag="ACHJWA_000895"
                     /pseudo
     CDS             1364..3937
                     /gene="lpfC"
                     /locus_tag="ACHJWA_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088424.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein LpfC"
     gene            3952..5007
                     /locus_tag="ACHJWA_000896"
     CDS             3952..5007
                     /locus_tag="ACHJWA_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4307546.1"
                     /translation="MKAAIALSLLGCVFGFSGKAFAGDAWGPCTPADGTTYHYNVDVD
                     VGIPDAAKNVAGTVLPDVLNWSNGQNVSLICECPDSYKNEKDTLVQGVSMLPPSGRTV
                     DSMKYYTLTEELEVATNIRISTSVYGFVPFKNQQALQTTGCNKVITTPYMGGAGLLSF
                     AITKPFIGDSVIPLTLIAELYASKTNKDYGTIPISSVSIQGRVTVTQDCEIKPGTVLD
                     VPFGEFPSSAFKNRQGQMPEGATEQEINLSFDCNNISDGIKVALRLEGATNADDPRAV
                     DMGNPDIGVLVKDSSGKILVPNDSSSTTLLNLSSLDSKTHRNAAIRLLALPISTTGKA
                     PKGGTFEGVTTIYLEME"
     gene            5012..5542
                     /gene="lpfE"
                     /locus_tag="ACHJWA_000897"
     CDS             5012..5542
                     /gene="lpfE"
                     /locus_tag="ACHJWA_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HFO4307547.1"
                     /translation="MKFKRLLHSGIASLSLVACGVNAATDLGPAGDIHFSITITTKAC
                     EMEKSDLEVDMGTMTLQKPAAVGTVLSKKDFTIELKECDGISKATVEMDSQSDSDDDS
                     MFALEAGGATGVALKIEDDKGTQQVPKGSSGTPIEWAIDGETTSLHYQASYVVVNTQA
                     TGGTANALVNFSITYE"
     gene            5797..7488
                     /gene="eptB"
                     /locus_tag="ACHJWA_000898"
     CDS             5797..7488
                     /gene="eptB"
                     /locus_tag="ACHJWA_000898"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312452.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFO4307548.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GISAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVNDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPVNAQ
                     AFAQLKKAADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKEAAAN"
     gene            7580..7656
                     /locus_tag="ACHJWA_000899"
     tRNA            7580..7656
                     /locus_tag="ACHJWA_000899"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7614..7616,aa:Pro,seq:cgg)
     gene            8401..10008
                     /gene="dppA"
                     /locus_tag="ACHJWA_000900"
     CDS             8401..10008
                     /gene="dppA"
                     /locus_tag="ACHJWA_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFO4307549.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            10159..11178
                     /gene="dppB"
                     /locus_tag="ACHJWA_000901"
     CDS             10159..11178
                     /gene="dppB"
                     /locus_tag="ACHJWA_000901"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFO4307550.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            11188..12090
                     /gene="dppC"
                     /locus_tag="ACHJWA_000902"
     CDS             11188..12090
                     /gene="dppC"
                     /locus_tag="ACHJWA_000902"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFO4307551.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            12101..13084
                     /gene="dppD"
                     /locus_tag="ACHJWA_000903"
     CDS             12101..13084
                     /gene="dppD"
                     /locus_tag="ACHJWA_000903"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFO4307552.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            13081..14085
                     /gene="dppF"
                     /locus_tag="ACHJWA_000904"
     CDS             13081..14085
                     /gene="dppF"
                     /locus_tag="ACHJWA_000904"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFO4307553.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKIGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(14115..15386)
                     /gene="yhjV"
                     /locus_tag="ACHJWA_000905"
     CDS             complement(14115..15386)
                     /gene="yhjV"
                     /locus_tag="ACHJWA_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFO4307554.1"
                     /translation="MQHNTLPKHDQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFTMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKTWLILLYGILLCLSPLLKLIE"
     gene            15862..15969
                     /gene="ldrD"
                     /locus_tag="ACHJWA_000906"
     CDS             15862..15969
                     /gene="ldrD"
                     /locus_tag="ACHJWA_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO4307555.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(16056..17735)
                     /gene="bcsG"
                     /locus_tag="ACHJWA_000907"
     CDS             complement(16056..17735)
                     /gene="bcsG"
                     /locus_tag="ACHJWA_000907"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFO4307556.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(17732..17923)
                     /gene="bcsF"
                     /locus_tag="ACHJWA_000908"
     CDS             complement(17732..17923)
                     /gene="bcsF"
                     /locus_tag="ACHJWA_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFO4307557.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(17920..19491)
                     /gene="bcsE"
                     /locus_tag="ACHJWA_000909"
     CDS             complement(17920..19491)
                     /gene="bcsE"
                     /locus_tag="ACHJWA_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFO4307558.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQYS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            19764..19952
                     /gene="bcsR"
                     /locus_tag="ACHJWA_000910"
     CDS             19764..19952
                     /gene="bcsR"
                     /locus_tag="ACHJWA_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFO4307559.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDILALKQAFSLPGIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            19964..20716
                     /gene="bcsQ"
                     /locus_tag="ACHJWA_000911"
                     /pseudo
     CDS             19964..20716
                     /gene="bcsQ"
                     /locus_tag="ACHJWA_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
     gene            20713..23337
                     /gene="bcsA"
                     /locus_tag="ACHJWA_000912"
     CDS             20713..23337
                     /gene="bcsA"
                     /locus_tag="ACHJWA_000912"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HFO4307560.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPRPLDPVRYLIQTCWLLIGASRKETPK
                     PRRRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTF
                     SLILALICVTQPFNPLAQFIFLMLLWGGALIVRRMPGRFSALMLIVLSLTVSCRYIWW
                     RYTSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPS
                     VDIFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIA
                     RTTHEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTVGWFLKEKQLAMMQTPH
                     HFFSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGG
                     IAVETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFR
                     LDNPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIAL
                     FVLPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAK
                     GGLVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLI
                     VLGGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQ
                     ILEGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTW
                     ALWQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPR
                     RPERSETAQPSDQALAQQ"
     gene            23348..25714
                     /gene="bcsB"
                     /locus_tag="ACHJWA_000913"
     CDS             23348..25714
                     /gene="bcsB"
                     /locus_tag="ACHJWA_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFO4307561.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAVLAVLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            25721..26827
                     /gene="bcsZ"
                     /locus_tag="ACHJWA_000914"
     CDS             25721..26827
                     /gene="bcsZ"
                     /locus_tag="ACHJWA_000914"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFO4307562.1"
                     /translation="MNVLRSGLVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            26809..30282
                     /gene="bcsC"
                     /locus_tag="ACHJWA_000915"
                     /pseudo
     CDS             26809..30282
                     /gene="bcsC"
                     /locus_tag="ACHJWA_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
     gene            30364..32352
                     /gene="hmsP"
                     /locus_tag="ACHJWA_000916"
     CDS             30364..32352
                     /gene="hmsP"
                     /locus_tag="ACHJWA_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFO4307563.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVHEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSISVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSVLQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            32535..33821
                     /gene="dctA"
                     /locus_tag="ACHJWA_000917"
     CDS             32535..33821
                     /gene="dctA"
                     /locus_tag="ACHJWA_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFO4307564.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDALNNRAPDGKTHELSS"
     gene            34042..35538
                     /gene="yhjJ"
                     /locus_tag="ACHJWA_000918"
     CDS             34042..35538
                     /gene="yhjJ"
                     /locus_tag="ACHJWA_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO4307565.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(35634..36563)
                     /gene="kdgK"
                     /locus_tag="ACHJWA_000919"
     CDS             complement(35634..36563)
                     /gene="kdgK"
                     /locus_tag="ACHJWA_000919"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFO4307566.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESDQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            36795..37562
                     /gene="pdeH"
                     /locus_tag="ACHJWA_000920"
     CDS             36795..37562
                     /gene="pdeH"
                     /locus_tag="ACHJWA_000920"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFO4307567.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTAVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            37632..39692
                     /gene="yhjG"
                     /locus_tag="ACHJWA_000921"
     CDS             37632..39692
                     /gene="yhjG"
                     /locus_tag="ACHJWA_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO4307568.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGIAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(39874..41196)
                     /gene="yhjE"
                     /locus_tag="ACHJWA_000922"
     CDS             complement(39874..41196)
                     /gene="yhjE"
                     /locus_tag="ACHJWA_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4307569.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLSAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(41608..42621)
                     /gene="yhjD"
                     /locus_tag="ACHJWA_000923"
     CDS             complement(41608..42621)
                     /gene="yhjD"
                     /locus_tag="ACHJWA_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFO4307570.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(42670..43569)
                     /gene="rcdB"
                     /locus_tag="ACHJWA_000924"
     CDS             complement(42670..43569)
                     /gene="rcdB"
                     /locus_tag="ACHJWA_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4307571.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLNELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPHLSEFLYQHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQNPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEYLD"
     gene            44089..44691
                     /gene="yhjB"
                     /locus_tag="ACHJWA_000925"
     CDS             44089..44691
                     /gene="yhjB"
                     /locus_tag="ACHJWA_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFO4307572.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGIMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            complement(44742..46391)
                     /gene="treF"
                     /locus_tag="ACHJWA_000926"
     CDS             complement(44742..46391)
                     /gene="treF"
                     /locus_tag="ACHJWA_000926"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFO4307573.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            46796..48193
                     /gene="ccp"
                     /locus_tag="ACHJWA_000927"
     CDS             46796..48193
                     /gene="ccp"
                     /locus_tag="ACHJWA_000927"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFO4307574.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLDVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            48404..49804
                     /gene="gadA"
                     /locus_tag="ACHJWA_000928"
     CDS             48404..49804
                     /gene="gadA"
                     /locus_tag="ACHJWA_000928"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFO4307575.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKEGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSD
                     HPKLQGIAQQNSFKHT"
     gene            50175..50999
                     /gene="gadX"
                     /locus_tag="ACHJWA_000929"
     CDS             50175..50999
                     /gene="gadX"
                     /locus_tag="ACHJWA_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFO4307576.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKVSSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDDHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDCSAEGIRL"
     gene            51367..52095
                     /gene="gadW"
                     /locus_tag="ACHJWA_000930"
     CDS             51367..52095
                     /gene="gadW"
                     /locus_tag="ACHJWA_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFO4307577.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(52458..55571)
                     /gene="mdtF"
                     /locus_tag="ACHJWA_000931"
     CDS             complement(52458..55571)
                     /gene="mdtF"
                     /locus_tag="ACHJWA_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFO4307578.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVIYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPNQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(55596..56705)
                     /gene="mdtE"
                     /locus_tag="ACHJWA_000932"
     CDS             complement(55596..56705)
                     /gene="mdtE"
                     /locus_tag="ACHJWA_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFO4307579.1"
                     /translation="MLTACDDKSAENTAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVKLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            56813..57091
                     /locus_tag="ACHJWA_000933"
     CDS             56813..57091
                     /locus_tag="ACHJWA_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4307580.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(57092..57619)
                     /gene="gadE"
                     /locus_tag="ACHJWA_000934"
     CDS             complement(57092..57619)
                     /gene="gadE"
                     /locus_tag="ACHJWA_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFO4307581.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(58418..58990)
                     /gene="hdeD"
                     /locus_tag="ACHJWA_000935"
     CDS             complement(58418..58990)
                     /gene="hdeD"
                     /locus_tag="ACHJWA_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFO4307582.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            59245..59577
                     /gene="hdeA"
                     /locus_tag="ACHJWA_000936"
     CDS             59245..59577
                     /gene="hdeA"
                     /locus_tag="ACHJWA_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFO4307583.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            59693..60019
                     /gene="hdeB"
                     /locus_tag="ACHJWA_000937"
     CDS             59693..60019
                     /gene="hdeB"
                     /locus_tag="ACHJWA_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFO4307584.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            60083..60730
                     /gene="yhiD"
                     /locus_tag="ACHJWA_000938"
     CDS             60083..60730
                     /gene="yhiD"
                     /locus_tag="ACHJWA_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFO4307585.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(60782..61552)
                     /locus_tag="ACHJWA_000939"
     CDS             complement(60782..61552)
                     /locus_tag="ACHJWA_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HFO4307586.1"
                     /translation="MISAQNLVYSLQGRRLTDNVSLTFPGGEIVAILGPNGAGKSTLL
                     RQLTGYLQPDSGECRLFNKPLNEWSITELAKHRAVMRQNSHMAFPFSVQEVIQMGRHP
                     HRTGNQDNETAQIMALCDCQALANRDYRQLSGGEQQRVQLARLLVQLWEPTPSPKWLF
                     LDEPTSALDIHHQQHLFRLLRQLVHERQFNVCCILHDLNLAAHYADRVVLMQKGKVIA
                     NGKPQDVLTQQALTMLYGADITVLKDPANHSPLIVLDH"
     gene            complement(61549..62541)
                     /gene="shuU"
                     /locus_tag="ACHJWA_000940"
                     /gene_synonym="chuU"
     CDS             complement(61549..62541)
                     /gene="shuU"
                     /locus_tag="ACHJWA_000940"
                     /gene_synonym="chuU"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease ShuU/ChuU"
                     /protein_id="HFO4307587.1"
                     /translation="MLKDSFSSARVFMGLSLLLLALVLFGASQGALKISFDALFDEEY
                     RDIWLNIRLPRVLLAVLVGAALATAGVIMQGLFRNPMADPGLLGVSSGSALMVGVAIV
                     LPFSFPVVLVLYEQMVFAIAGSLVVCAIIFLITQRHRDGSMMQLLLAGIAINALCGAA
                     IGILSYIGDEQQLRQLTLWMMGNLGQAQWPTLLVASSFILPAIIATTCLAGTLNLLQL
                     GDEEAHYLGVNVKRKRQQLLLVSSLLVGAAVSVSGIIGFIGLVIPHLIRMTTGANHRW
                     LIPCSALAGACLLLMADTLARTLVQPAEMPVGLLTSLLGGPYFMWLILRNRRIT"
     gene            complement(62590..63213)
                     /gene="chuY"
                     /locus_tag="ACHJWA_000941"
     CDS             complement(62590..63213)
                     /gene="chuY"
                     /locus_tag="ACHJWA_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobilin reductase"
                     /protein_id="HFO4307588.1"
                     /translation="MTPWLLFGAGGKGVGARTLELALAEQRPVVAVIRHADAATKLAQ
                     QGVQVFTGDACDASVVAAACRAAGPDALIISTMGGAQDYLAHRTVIDEAEKAGISRMI
                     LVTSLGCGDSWPFLSERAKAAFGQAVREKTLAESWLQTSQLDYAILRPGGLLDGAATG
                     KAQRIQNQECHGFVRRADVAAHIHELANAPALNQQVYSLIEPDLKPA"
     gene            complement(63213..63707)
                     /gene="hutX"
                     /locus_tag="ACHJWA_000942"
     CDS             complement(63213..63707)
                     /gene="hutX"
                     /locus_tag="ACHJWA_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HFO4307589.1"
                     /translation="MSHVSLQEFLKTEPDGTLEVVAEQYNTTLLEVVRNLPSSTVVPG
                     DKFDTVWDTVCEWGNVTTLVHTADVILEFSGELPSGFHRHGYFNLRGKHGMSGHIKAE
                     NCTHIALIERKFMGMDTASILFFNKEGSAMLKIFLGRDDHRQLLSEQVSAFHTLAASL
                     KEHA"
     gene            complement(63720..65057)
                     /gene="hutW"
                     /locus_tag="ACHJWA_000943"
     CDS             complement(63720..65057)
                     /gene="hutW"
                     /locus_tag="ACHJWA_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HFO4307590.1"
                     /translation="MNANNTLDLTPHFALDGDQPFKDRRAMMPFRGAIPVAKEQLAQT
                     WQEMINQTASPRKRLVYLHIPFCATHCTFCGFYQNRFNEDACAHYTDALIREIEMEAD
                     SVLHQSAPIHAVYFGGGTPSALSAHDLARIITTLREKLPLAPDCEITIEGRVLNFDAE
                     RIDACLDAGANRFSIGIQSFNSKIRKKMARTSDGPTAIAFMESLVKRDRAAVVCDLLF
                     GLPGQDAQTWGEDLAIARDIGLDGVDLYALNVLSNTPLGKAVENGRTTVPSPAERRDL
                     YLQGCDFMDDAGWRCISNSHWGRTTRERNLYNLLIKQGADCLAFGSGAGGSINGYSWM
                     NERNLQTWHESVAAGKKPLMLIMRNAERNAQWRHTLQSGVETARVPLDELTPHAEKLA
                     PLLAQWHQKGLSRDASTCLRLTNEGRFWASNILQSLNELIQVLNAPAIMREKP"
     gene            complement(65077..65991)
                     /gene="shuT"
                     /locus_tag="ACHJWA_000944"
     CDS             complement(65077..65991)
                     /gene="shuT"
                     /locus_tag="ACHJWA_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     ShuT"
                     /protein_id="HFO4307591.1"
                     /translation="MPRIITRPFLFSPLTLCISAVASAAKTAVKRKKLFTAVLALSWA
                     FSVTAAERIVVAGGSLTELIYAMGAGERVVGVDETTSYPPETAKLPHIGYWKQLSSEG
                     ILSLRPDSVITWQDAGPQIVLDQLRAQKVNVVTLPRVPATLQQMYANIRQLAKTLQVP
                     EQGEALVTQISQRLERVQQNVATKKAPVKAMFILSAGGSAPQVAGKGSVADAILSLAG
                     AENVATHQQYKSYSAESLIAANPEVIVVTSQMVDGDINRLRSIAGITHTAAWKNQRII
                     TVDQNLILGMGPRIADVVESLHQQLWPQ"
     gene            66674..68656
                     /gene="chuA"
                     /locus_tag="ACHJWA_000945"
                     /gene_synonym="shuA"
     CDS             66674..68656
                     /gene="chuA"
                     /locus_tag="ACHJWA_000945"
                     /gene_synonym="shuA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent heme/hemoglobin receptor
                     ChuA/ShuA"
                     /protein_id="HFO4307592.1"
                     /translation="MSRPQFTSLRLSLLALAVSATLPTFAFATETMTVTATGNARSSF
                     EAPMMVSVIDTSAPENQTATSATDLLRHVPGITLDGTGRTNGQDVNMRGYDHRGVLVL
                     VDGVRQGTDTGHLNGTFLDPALIKRVEIVRGPSALLYGSGALGGVISYDTVDAKDLLQ
                     EGQSSGFRVFGTGGTGDHSLGLGASAFGRTENLDGIVAWSSRDRGDLRQSNGETAPND
                     ESINNMLAKGTWQIDSAQSLSGLVRYYNNDAREPKNPQTVEASESSNPMVDRSTIQRD
                     AQLSYKLAPQGNDWLNADAKIYWSEVRINAQNTGSSGEYREQITKGARLENRSTLFAD
                     SFASHLLTYGGEYYRQEQHPGGATTGFPQAKIDFSSGWLQDEITLRDLPITLLGGTRY
                     DSYRGSSDGYKDVDADKWSSRAGMTINPTNWLMLFGSYAQAFRAPTMGEMYNDSKHFS
                     IGRFYTNYWVPNPNLRPETNETQEYGFGLRFDDLMLSNDALEFKASYFDTKAKDYIST
                     TVDFAAATTMSYNVPNAKIWGWDVMTKYTTDLFSLDVAYNRTRGKDTDTGEYISSINP
                     DTVTSTLNIPIAHSGFSVGWVGTFADRSTHISSSYSKQPGYGVNDFYVSYQGQQALKG
                     MTTTLVLGNAFDKEYWSPQGIPQDGRNGKIFVSYQW"
     gene            68705..69733
                     /gene="chuS"
                     /locus_tag="ACHJWA_000946"
     CDS             68705..69733
                     /gene="chuS"
                     /locus_tag="ACHJWA_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hematinate-forming heme oxygenase ChuS"
                     /protein_id="HFO4307593.1"
                     /translation="MNHYTRWLELKEQNPGKYARDIAGLMNIREAELAFARVTHDAWR
                     MHGDIREILAALESVGETKCICRNEYAVHEQVGTFTNQHLNGHAGLILNPRALDLRLF
                     LNQWASVFHIKENTARGERQSIQFFDHQGDALLKVYATDNTDMAAWSELLARFITDEN
                     TPLELKAVDAPVVQTRADATVVEQEWRAMTDVHQFFTLLKRHNLTRQQAFNLVADDLA
                     CKVSNSALAQILESAQQDGNEIMVFVGNRGCVQIFTGVVEKVVPMKGWLNIFNPTFTL
                     HLLEESIAEAWVTRKPTSDGYVTSLELFAHDGTQIAQLYGQRTEGEQEQAQWRKQIAS
                     LIPEGVAA"
     gene            complement(69794..70324)
                     /gene="dctR"
                     /locus_tag="ACHJWA_000947"
     CDS             complement(69794..70324)
                     /gene="dctR"
                     /locus_tag="ACHJWA_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFO4307594.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(70480..71046)
                     /gene="slp"
                     /locus_tag="ACHJWA_000948"
     CDS             complement(70480..71046)
                     /gene="slp"
                     /locus_tag="ACHJWA_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFO4307595.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT        17684 a        17351 c        18639 g        17554 t
ORIGIN      
        1 tgtcgatttt aatacatcaa gattttcttt ttaatgtaat tttttaaatg gagtttttca
       61 tgaaaaaggt cgtttttgcc ttaaccgcac tggcgttaac ttctggcact gtattcgcag
      121 cagagtctgg tgacggcacg gttaaattta caggcgagat cgtggattca ccttgcgtac
      181 tgtccgttga ctctcagaac caggaagttg tgttgggtca ggtacaaaaa tctgtatttg
      241 ctgcggttgg tgacaaatcc ccggcaaaac ctttcgaaat caaactggaa gattgcgata
      301 ccaccacgat gaaaaaagcc aatgtgagct ttagtggtgt tggtgatgca gataaatctg
      361 atctgatttc tgttagcact gaagcgggcg cggccaaagg tgttggtatc ggtatttatg
      421 ataacagcaa taccctggtc gctcttaacg gtggtaaagc aagtgttgat ctgagtaaag
      481 gccagacggt tctctacttt accgctaact acgtttctac tctggcaacg gtaacgaccg
      541 gttacggcaa tgcgcaggtc gatttcaacc tgtcttacga gtaatcgtcc gttttacaaa
      601 tggcggcgta attgccgcca ttagtcgtca aagttcatgg atcgaatgat gaaaagtaaa
      661 tttgttgcgt tggctctctc tctgttcctt tcacagtcag ttctggcggg tggcgtcgga
      721 cttagctcta ctcgcgtaat ttatgatggc agtaagaaag aagcctcgct gaccgtacag
      781 aataaaagta agacggaaga gtttcttatc caatcctggg tggatgatgc cgcgggcagc
      841 aaaaaaacac cgtttattat tacgccccca ctattcaagc ttgatccaga aaagaacaat
      901 attttgcgta ttgtgaatat tacgcccggc ctgcctcagg atcgcgaatc ggtctactgg
      961 gttaatgtga aagccattcc atctaaaagt gacgacagcg aaaacaaaaa tgttttgcag
     1021 attgccgttc gcacccgaat caaacttttt tatcgcccgg caggtttaaa gggtgatgta
     1081 aaaaccgcgc caaatgaact gcgatttacg cgtaacggta atcagctccg cgttgataat
     1141 ccgacagttt ttaatattac ctttaatcaa ttttttgcga atgacaaaga aattgaaaaa
     1201 gcaggcatgg tacccgctaa aggggcatta aatatcacat tacccgccgg ggtaggtagc
     1261 gtttctaaaa ttaaatataa caccatcaat gactttggat cgtcagcgga aatgatcact
     1321 aaaaatgttg attgattaat atcttttcag gagaaatagc tcaatgtcac ggaagaccgt
     1381 ttcacgtact ttttcttcct tttctatctc tgtggtggcg gttgctgttg ccagcacgtt
     1441 ttctgcacat gccggaaagt ttaaccccaa attcctggag gacgttcagg gtgtgggcca
     1501 gcatgtcgat ttaacgatgt tcgaaaaagg ccaggaacag cagttacccg gcatctatcg
     1561 tgtctctgtt tacgtcaatg aacagagaat ggaaacccgc acactggaat ttaaagaggc
     1621 cacagaagca cagcgcaaag cgatggggga atcccttgtg ccttgcctta gccggacgca
     1681 actggcagag atgggcgtgc gcgttgaaag ctttccggcc ctcaatctgg tgtctgccga
     1741 agcctgcgta ccgtttgacg aaatcattcc cctggcctca agccatttcg acttcagtga
     1801 acaaaagctg gtattaagtt tccctcaggc ggcgatgcac caggtggcgc gcggcacggt
     1861 gccggaatct ttatgggacg aaggtatccc tgcgttgctg ctggattaca gttttagcgg
     1921 gagcaacagt gaatatgact cgacggggtc ttccagcagt tatgtcgatg ataatggcac
     1981 tgttcatcat gatgatggta aagatacgct aaagagcgac agctactacc tcaacttacg
     2041 cagcgggctg aaccttggcg cttggcggtt acggaattac agtacctgga gccattccgg
     2101 tggcaaagcg cagtgggata atatcggtac gtctttgagt agggccatca tccctttcaa
     2161 agcgcaatta acaatggggg atacggcgac cgcgggggac atttttgaca gcgttcagat
     2221 gcgtggcgcg atgttagcct ctgatgaaga gatgctgccc gacagccagc gaggctttgc
     2281 acccatcgtt cgagggatcg ccaaaagcaa cgccgaagtc agtattgaac aaaacggcta
     2341 cgttatttat cgcacctatg tgcagcctgg tgcctttgaa ataaacgatc tttaccccac
     2401 tgcaaacagc ggtgatctca cggtcattat taaagaagcg gatggcagtg aacaacgctt
     2461 tatttagcca ttctcatcgg tgccaatttt ccagcgtgaa ggccatctca aatatagctt
     2521 tgccgcaggt gaatatcagg ccgggaatta tgacagcgcc tcgccgcgtt tcgggcagct
     2581 tgatctgatc tacggtttac cgtgggggat gacggcctac ggcggcgtat taatctctaa
     2641 taattacaat gcatttacat tagggatagg gaaaaacttt ggttatatcg gggcgatttc
     2701 cattgatgtg acgcaggcta aaagcgaact gaataacgat cgcgatagcc agggacaatc
     2761 ttatcgtttc ttatattcca agagcttcga aagcggcacc gatttccgcc ttgcgggcta
     2821 tcggtactct accagcggtt tctatacctt ccaggaagcc accgatgtgc gcagtgacgc
     2881 tgacagcgac tataaccgtt atcacaagcg cagcgaaata cagggtaacc tgacgtagca
     2941 attaggggcc tatggctctg tttatttaaa tttaacgcag caggattact ggaacgacgc
     3001 aggtaaacag aacacggtat cggcgggtta caacggacgt attggcaagg tcagttacag
     3061 tattgcatat agctggaata aaagccctga atgggatgaa agcgatcgct tgtggtcttt
     3121 caatatttcc gttccactag gccgggcctg gagtaactat cgcgtcacga ccgaccagga
     3181 tggtcgtacc aatcaacagg ttggggtcag cggaacgctg cttgaggatc gcaacctgag
     3241 ctacagtgtc caggaaggct acgccagcaa cggtgtgggt aacagcggta acgctaacgt
     3301 tggctatcag ggtgggtccg gtaatgtcaa cgtaggctat agctacggga aagattaccg
     3361 gcagctcaac tacagcgttc gcggcggcgt gatagttcat agcgaaggcg tgacgctttc
     3421 ccaaccgcta ggcgaaacca tgacgctcat ctccgtaccc ggtgcgcgca atgcccgcgt
     3481 ggtgaataac ggcggcgttc aggttgactg gatgggtaac gcgatcgtgc cttatgccat
     3541 gccgtatcgt gaaaacgaaa tctcactgcg tagcgattcg ttgggtgacg atgttgacgt
     3601 tgaaaatgcg ttccagaaag tggtgccaac gcgtggagcg attgtcagag cgcgttttga
     3661 tacccgcgtt ggttaccgcg tattaatgac gctgcttcgt tccgcgggca gcccggtgcc
     3721 ctttggagca acggcaacgc taatcaccga taaacaaaac gaggtgagca gtatcgttgg
     3781 tgaagaagga cagctctata ttagcggaat gccagaggaa ggacgggtat tgattaaatg
     3841 gggtaatgac gcgtcgcagc aatgcgtggc gccttataaa ttatccctgg aattaaaaca
     3901 gggcggaatt attcctgttt cggccaattg ccagtaagca aggagagcga aatgaaagcg
     3961 gcaatagcat tatccttatt aggatgcgta ttcggatttt ctggcaaggc atttgccgga
     4021 gatgcgtggg ggccatgtac ccccgcggat ggcacgactt atcactataa tgtcgatgtg
     4081 gatgttggta tacctgatgc ggcaaaaaac gtcgcaggaa cggttttacc ggatgtcctt
     4141 aactggtcta atggacaaaa cgtatcgcta atttgtgaat gtcctgattc ttataagaat
     4201 gaaaaagata ctctagtgca gggggtgagt atgcttcccc cttctggccg tacggttgac
     4261 agtatgaaat attatacttt gacggaggaa ctggaggttg cgacgaacat tcgcatcagt
     4321 accagtgtgt atgggtttgt tcctttcaaa aaccagcagg cgctgcaaac aaccggatgc
     4381 aacaaagtca ttactacgcc ctatatgggc ggcgcaggtc tactttcctt tgctattact
     4441 aaacccttta tcggcgattc cgtcatccct ctgacgttaa ttgccgaact gtatgcctcg
     4501 aaaacaaata aagattacgg aacaataccc atatcttccg tatctattca ggggcgtgtc
     4561 accgtaaccc aggattgcga aattaaaccc ggcacggtgc tggatgtgcc atttggtgaa
     4621 ttcccctcct cggcgtttaa aaacaggcaa gggcaaatgc ctgaaggtgc gacagagcaa
     4681 gaaattaacc tcagcttcga ttgcaacaat atctctgatg gtatcaaggt tgcgttgcgc
     4741 cttgaggggg caaccaatgc tgacgatccc cgagcggtgg atatgggcaa ccccgatatt
     4801 ggcgtactgg tgaaagattc cagcggcaaa attttggtac cgaatgattc aagcagcacc
     4861 acgttattaa atttgagcag cctggattct aaaactcacc gcaatgccgc gattcgactg
     4921 ctggctttac cgattagcac gacgggtaaa gcgcctaaag gcgggacgtt tgaaggcgta
     4981 acgaccatat atcttgaaat ggagtgatgc catgaagttc aaacgattgc tgcatagcgg
     5041 catcgccagt ttgagtctgg ttgcctgcgg ggtgaatgcg gcgacggatc ttggcccggc
     5101 aggggatatt catttctcca tcactatcac cactaaagct tgcgagatgg aaaaaagcga
     5161 tctcgaagtc gatatgggaa caatgacgct gcaaaaacct gcggcagtcg gtacggtgtt
     5221 gagcaagaaa gatttcacca ttgaactcaa agagtgcgat gggatatcca aagcgaccgt
     5281 tgagatggac agtcagtcgg acagcgatga tgattccatg tttgcccttg aggctggtgg
     5341 cgcaacgggt gttgcgttga agatagagga cgataaagga acgcagcaag ttcccaaagg
     5401 ctccagcgga acgccgattg aatgggcgat tgatggcgaa accacgtcgc ttcactacca
     5461 ggcgagttat gtggtcgtca acactcaggc cactggtggc acagcgaatg cccttgtcaa
     5521 tttttccatc acctatgagt aagttactgg gccagcatcc tggcccaacc tatctctgaa
     5581 tagtcgttaa tccgttaaaa tggtctattc cggacttgca cttttgtaaa tttgtgcttc
     5641 atgcacactc tttccccaca ctttttccct ttgctgtggt ctacttattc gcgcgtgtag
     5701 attttactta tctgactacc tccgcacttt tcccctgctg ggcctgaaaa gccgctaagc
     5761 agggtgttat cacctgtttg tccagggttt gtttgcatga gatacatcaa atcgattaca
     5821 cagcagaagc tgagcttttt gcttgcaatc tatattggcc tttttatgaa tggcgcggtt
     5881 ttttaccgcc gcttcggcag ctatgcgcac gattttaccg tctggaaagg catttctgct
     5941 gttgttgaac tggccgccac cgtactggtg accttctttt tactacgtct tctttcgctg
     6001 tttggccgcc gcagctggcg tattctggca tcgctggttg tgctcttttc cgcaggtgcc
     6061 agctattaca tgaccttcct taatgtggtc attggttatg gcatcatcgc ttccgtcatg
     6121 accaccgata tcgacctgtc aaaagaagtt gttggtctga actttattct ctggttaatc
     6181 gccgttagcg cattgcctct tatccttatc tggaataacc gctgtcgcta caccttgctc
     6241 cgacaactgc gaaccccagg gcagcgtatt cgcagcctgg cggtcgtcgt actggcggga
     6301 attatggttt gggcaccgat tcgtttgctg gatatccagc agaagaaagt ggagagggcg
     6361 accggcgttg atttgccgag ttatggcggt gtcgtagcga actcttatct gccatcaaac
     6421 tggctttctg cgttggggct gtacgcctgg gcgcgggtcg atgaatcttc cgataataat
     6481 tcattgctta acccggcgaa gaaattcact tatcaggcac cgcaaaatgt taatgatact
     6541 tatgtcgtgt ttatcatcgg tgaaaccacg cgttgggacc atatgggcat tttcggctat
     6601 gagcgtaata ccacgccgaa gctggcccag gagaaaaatc tggcggcgtt ccgtggttac
     6661 tcctgtgata ccgcaaccaa actctctctg cgttgcatgt ttgtacgtca ggggggcgcg
     6721 gaagataatc cgcagcgcac attaaaagaa cagaacattt tcgcggtact gaagcagtta
     6781 ggattcagtt ctgacctcta cgctatgcag agcgaaatgt ggttctacag caatacgatg
     6841 gcggacaata ttgcttaccg tgaacagatt ggtgcggagc cacgtaaccg tggcaagccg
     6901 gtagatgata tgttgctggt agacgaaatg cagcaatcgc tggggcgcaa cccggatggt
     6961 aagcatctaa tcattctgca taccaaaggt tcgcacttta actacaccca gcgttacccg
     7021 cgcagctttg cgcagtggaa gccggaatgt attggtgttg atagcggatg taccaaagcg
     7081 cagatgatca actcctatga caactcggtg acctatgtgg atcactttat ctccagtgtg
     7141 attgatcagg ttcgcgataa gaaagcgatt gtgttctacg cagctgacca cggcgagtca
     7201 attaatgaac gcgagcacct gcacggcacg ccgcgtgaac tggcaccgcc ggagcagttc
     7261 cgcgtaccga tgatggtctg gatgtcagat aaatatctgg aaaatccggt caatgcgcag
     7321 gcgtttgcgc agctgaaaaa agcagccgac atgaaagtgc cacgccgtca cgtagagctg
     7381 tacgacacca tcatgggttg tcttggctat acttcaccgg atggtggaat taacgaaaac
     7441 aacaactggt gtcacatccc gcagacaaaa gaggcagcgg ctaactaaga ggcctgctga
     7501 ctttctcgcc gatcaaaagg cattttgcta ttaagggatt gacgagggcg tatctgcgca
     7561 gtaagatgcg ccccgcattc ggtgattggc gcagcctggt agcgcacttc gttcgggacg
     7621 aaggggtcgg aggttcgaat cctctatcac cgaccaaatt cgaaaagcct gctcaacgag
     7681 caggcttttt tgcatctgca gcccatgagg aagagaacct ccgggggcag ggaggttcga
     7741 ctcgacgtag gcctgataag acgcgtcagc gtcgcatcag gcaacggcac cgcacttatt
     7801 gtcggatgcg gcgtgaacgc cttattcgac ctacacgcca cacatttccc tgccaaattt
     7861 gcgccccata tctcactatc cattaacgga tttgtgacag aaaccattgt ctttttttat
     7921 atttgcttaa atcttttttt gcgttactta tggctggcgc tggcattttc cgctgtgcgt
     7981 tttagcgttc atggtattaa tcgctcagat atccaccttt tcctcagatt tttttcacta
     8041 aatgcataaa acttaatcac caaatgttgc taaatattaa gctgattgtt ctggtcatgt
     8101 tggagtagca caaatttctc tgctgcaaat agctgtttga agttttttta atctttgttt
     8161 gtggattctc accgttggtg taaatcccgg ttggctgtat tgacgttttc acattctgtt
     8221 gacagattgt aggtcacgag gggcatttta tggaggatcc gcactgttac actgatgtta
     8281 attagtacgg catccccacc tcataacgtt gacccgaccg ggcaaaaaac aaaaaaggtc
     8341 aggcagcgac aacccactgc aaagggttaa aacaacaaac atcacaattg gagcagaata
     8401 atgcgtattt ccttgaaaaa gtcagggatg ctgaagcttg gtctcagcct ggtggctatg
     8461 accgtcgcag caagtgttca ggctaaaact ctggtttatt gctcagaagg atctccggaa
     8521 gggtttaacc cgcagctgtt tacctccggt accacctatg acgcctcttc cgtaccgctt
     8581 tataaccgcc tagttgaatt taaaatcggc accaccgaag tgattccggg cctcgctgaa
     8641 aagtgggaag tcagcgaaga cggtaaaacc tataccttcc atctgcgtaa aggtgtgaag
     8701 tggcacgaca ataaagaatt caaaccgacg cgcgaaatga atgccgatga cgtggtgttc
     8761 tcgttcgatc gtcagaaaaa cgcgcaaaac ccgtaccata aagtttctgg cggcagctac
     8821 gaatacttcg aaggcatggg cttgccggag ctgatcagcg aagtgaaaaa ggtggacgac
     8881 aacaccgttc agtttgtgct gactcgcccg gaagcgccgt tcctcgctga cctggcaatg
     8941 gacttcgcct ctattctgtc aaaagaatat gctgatgcga tgatgaaagc cggtacaccg
     9001 gaaaaactgg acctcaaccc aatcggcacc ggtccgttcc agttacagca gtatcaaaaa
     9061 gattcccgta tccgctacaa agcgtttgat ggctactggg gcaccaaacc gcagatcgat
     9121 acgctggttt tctctattac ccctgacgct tccgtgcgtt acgctaaatt gcagaaaaat
     9181 gaatgccagg tgatgccgta cccgaacccg gcagatatcg cccgcatgaa gcaggataaa
     9241 tctatcaacc tgatggaaat gccggggctg aacgtcggtt atctctcgta taacgtgcag
     9301 aaaaaaccac tcgatgacgt gaaagttcgc caggctctga cctacgcggt gaacaaagac
     9361 gcgatcatca aagcggttta tcagggcgcg ggtgtatcag cgaaaaacct gatcccgcca
     9421 accatgtggg gctataacga cgacgttcag gactacacct acgatcctga aaaagcgaaa
     9481 gccttgctga aagaagcggg tctggaaaaa ggtttctcca tcgacctgtg ggcgatgccg
     9541 gtacaacgtc cgtataaccc gaacgctcgc cgcatggcgg agatgattca ggcagactgg
     9601 gcgaaagtcg gcgtgcaggc caaaattgtc acctacgaat ggggtgagta cctcaagcgt
     9661 gcgaaagatg gcgagcacca gacggtaatg atgggctgga ctggcgataa cggggatccg
     9721 gataacttct tcgccactct gttcagctgc gccgcctctg aacaaggctc caactactca
     9781 aaatggtgct acaaaccgtt tgaagatctg attcaaccgg cgcgtgctac cgacgaccat
     9841 aacaaacgcg ttgaactgta caagcaagcg caggtggtga tgcacgatca ggctccggca
     9901 ctgatcatcg ctcactccac cgtgtttgaa ccggtacgca aagaagttaa aggctatgtg
     9961 gttgatccat taggcaaaca tcacttcgaa aacgtctcta tcgaataatt aaaagccata
    10021 cccggtggtg ctgtgcctct gcgtgcagcg ccatccggca gcaatacttc attccctgcc
    10081 cgacctgggc gggaattgat ttgtgagcaa tacagacacg cagttccagg ctgcgggtca
    10141 ctacagagaa tccgggttat gttgcagttt attctccgac gtttgggact cgtcatcccc
    10201 acgtttatcg gtattaccct tctcacattt gcctttgtcc acatgatccc gggcgatcct
    10261 gtgatgatca tggcgggcga acgtgggatc tccccagagc gtcacgcgca gttgctggct
    10321 gaactcggct tagataaacc gatgtggcag cagtatctcc attacatttg gggcgttatg
    10381 catggcgatc taggcatttc aatgaaaagc cgaattccgg tatgggaaga gttcgtgccg
    10441 cgctttcagg ccacgctgga acttggcgtc tgcgcgatga tttttgcgac cgcggtcggc
    10501 attccggttg gtgtgctggc cgcggttaaa cgcggttcca ttttcgatca cacagcggtt
    10561 ggcctggcgc tgacaggtta ttcaatgcct atcttctggt ggggcatgat gctgatcatg
    10621 ctggtttcgg tgcactggaa cctgacaccc gtctccggtc gcgtgagcga tatggtgttc
    10681 ctcgatgatt ccaatccgtt aaccggtttt atgctgatcg acaccgccat ctggggtgaa
    10741 gacggtaact ttatcgatgc cgtcgcccat atgatcttgc ccgccattgt gctgggtact
    10801 attccgctgg cggtcattgt gcgtatgaca cgctcctcga tgctggaagt gctgggtgaa
    10861 gattacatcc gcaccgcgcg cgccaaaggg ttgacccgca tgcgggtgat tatcgtccat
    10921 gcgctgcgta acgcgatgct gccggtagtg accgttatcg gcctccaggt gggaacattg
    10981 ctggcggggg cgattctgac cgaaaccatc ttctcgtggc ccggtctggg gcgctggttg
    11041 attgacgcac tgcaacggcg cgattatccg gtggtgcagg gcggcgtatt gctggtggcg
    11101 acgatgatta tcctcgtcaa cctgctggtc gatctgctgt atggcgtggt gaacccgcgt
    11161 attcgtcata agaagtaagg ggtcatcatg tcacaggtta ctgaaaataa agtgattagc
    11221 gcgccggtac cgatgacccc tttacaggag ttctggcact attttaaacg caacaaaggc
    11281 gcggtcgtcg ggctggttta cgtcgtcatc gtgctgttta tcgcgatctt tgccaactgg
    11341 attgctccct ataacccggc ggaacagttc cgcgatgcac tgctcgcccc gccagcctgg
    11401 caggaaggcg gcagcatggc gcacttgctg ggtaccgatg atgtaggccg tgatgtgctg
    11461 tcgcggctga tgtacggcgc gcgcttgtcg ctgctggttg gctgtctggt ggtggtgtta
    11521 tcgctgatta tgggcgttat tctcggcctg atcgccggtt acttcggcgg cctggtcgat
    11581 aacatcatta tgcgcgtggt cgatatcatg ctggcgctgc caagtctgct gttggcactg
    11641 gtgctggtgg cgattttcgg cccgtcgatt ggtaacgccg cgctggcgct gacctttgtt
    11701 gccttgccgc actatgtgcg cttaacccgc gccgccgtgc tggtggaggt gaatcgcgat
    11761 tacgtcaccg cttctcgcgt ggcaggagcc ggggcgatgc gccagatgtt tattaacatc
    11821 ttcccgaact gccttgcacc gctgattgtt caggcgtcgc tcggtttctc taacgccatt
    11881 ctcgatatgg ctgctcttgg tttcctcggc atgggggcgc agccgccaac gccggagtgg
    11941 ggcaccatgc tctccgacgt gttgcagttc gcgcaaagcg cctggtgggt cgtgaccttc
    12001 ccgggtctgg cgatcctgct gacggtgctg gcatttaacc tgatgggtga cggtctgcgt
    12061 gacgcgctcg atcccaaact gaagcagtaa gaggttcgag atggcgttat taaatgtaga
    12121 taaattatcg gtgcatttcg gcgacgaaag cgcaccgttc cgcgccgtag accgcattag
    12181 ctacagcgta aaacagggcg aagtagtcgg gattgtgggt gagtctggct ccggtaagtc
    12241 ggtcagttcg ctggcgatta tggggctgat tgattatccg ggccgcgtga tggcggaaaa
    12301 gctggagttt aacggtcagg atttgcagcg tatctcggaa aaagagcgcc gcaacctggt
    12361 gggtgccgaa gtggcgatga tcttccagga cccgatgacc agccttaacc cgtgctacac
    12421 cgtgggtttc cagattatgg aagcgattaa ggtgcatcag ggcggtaata agagcactcg
    12481 tcgtcagcgg gcgattgact tgttgaatca ggtcggtatt cccgatccgg cttcgcgtct
    12541 ggatgtttac ccgcatcagc tttccggcgg catgagccag cgcgtgatga ttgcgatggc
    12601 cattgcctgt cggccaaaac tgctgattgc cgatgaacca accactgcgc tggacgtgac
    12661 cattcaggcg caaatcattg aactactgct ggagctacag cagaaagaga acatggcgct
    12721 ggtgttaatt acccatgacc tggcgctggt ggcggaagcg gcacataaaa tcatcgtgat
    12781 gtatgccggt caggtggtgg aaacgggcga tgcgcacgcc atcttccatg cgccgcgtca
    12841 cccgtatact caggcattgc tgcgtgcgct gccggaattt gctcaggaca aagaacgtct
    12901 ggcatcgttg cctggcgttg ttcccggcaa gtacgaccgc ccgaacggct gcttgcttaa
    12961 cccgcgctgc ccctatgcca ctgacagatg tcgcgctgaa gaaccggcgc tgaatatgct
    13021 cgctgacggg cgtcagtcca aatgccatta cccacttgat gatgccggga ggccgacact
    13081 atgagtacgc aagaggccac ctcgcaacaa ccgctgttgc aggctatcga cctgaaaaaa
    13141 cattatccgg tgaagaaagg tatgttcgct ccggaacgtc tggtgaaggc gctggacggc
    13201 gtttcgttta accttgaacg tggcaaaacg ctggcggtag taggtgaatc tggctgcggt
    13261 aaatcgaccc tcggtcggtt gctgacgatg attgaaacgc ccaccggcgg cgagctgtat
    13321 taccaggggc aggatctgct taagcacgat ccgcaggcgc agaagctgcg tcggcagaaa
    13381 atccagattg tcttccagaa cccttacggt tcgctgaatc cgcgtaaaaa aatcgggcaa
    13441 attcttgaag agccgctgct gatcaacacc agcttaagca aagaacagcg tcgggaaaaa
    13501 gccctgtcga tgatggcgaa agtcggcctg aaaaccgagc actatgaccg ctatccgcat
    13561 atgttctccg gcggtcagcg tcagcgtatc gccattgccc gaggtctgat gctcgacccg
    13621 gacgtggtga ttgccgatga accggtctcc gcgctggacg tctcggtgcg tgcacaggtg
    13681 ctcaacctga tgatggattt gcagcaggaa ctggggcttt cttatgtctt catctcccac
    13741 gacctgtcgg tggtggagca cattgctgat gaagtgatgg tgatgtacct gggccgctgc
    13801 gtggagaagg gaacgaaaga ccaaatcttc aataacccgc gccatccgta cactcaggcg
    13861 ctactttccg cgacgccgcg cctgaatccg gacgatcgcc gcgagcgcat caaactcacc
    13921 ggtgaactgc caagcccgct gaatccaccg ccgggctgcg cgtttaacgc ccgctgtcgt
    13981 cggcgcttcg gcccctgcac ccagttgcag ccgcagctaa aagactacgg cggtcaactg
    14041 gtagcttgtt ttgctgttga tcaggatgaa aatccgcagc gttaactcac aaaaccgggg
    14101 ccatgcgctc cggtttactc aatcagcttc aacagtggcg acaagcatag caaaatgccg
    14161 taaagcagaa tcagccaggt tttaaatccg cgaagttttt ccagttgtgc gactttatag
    14221 atcaggaaaa acggaatgag gcacgacaca atgccatata acgggcttcc caactgaaag
    14281 aacaccagca ccgatacacg aaacgaaacc caaatcgtca acgtaatgac gataaaagcg
    14341 cagatcgcca gagtcagcac gcgtgagtta attttcttgg tatcaataat tcggcttaac
    14401 agattgagaa taatgccttt aatggcctcg tggaaaccga gataaatgcc aaagaatgcg
    14461 gtcagtacgg caaagatatt cagcaccgta gaggtgatat gaatgatatg cccagggatc
    14521 acctgcgcgg ccagtgccag tgccgagata ttttgttcaa aggcagaaac ggcttcttcg
    14581 tggctaattg agaaggtaaa cgaaaaggca aaaaacagta tcaccgcgat gagcgtgata
    14641 taactaattc ggtgggtacg cagcgcgagc cgggttgcca gtactttatc cgcttcccgt
    14701 ttacgatagg caatattcat tgggttaagt acctgaataa atactgcaga aaagaagcaa
    14761 aatggaatgg taagcaggac atcgcgaaaa aagacggagg cttgcgggaa ggcggttata
    14821 ttggcgaaat tccagtgcgg gatcatcgta aaaccgaaca cgacaataat ccctactttg
    14881 accaccacca ttggcccaga aatcttaaac agtaatcgtt caccaccaga cgcaatcgct
    14941 accagtacgg cgaaaatagc gactttataa agtagagatt gtgaaagatc ggcatcggtt
    15001 aaaccgaagg ttttcaggta cgaggcgctg tcgaaaacca cggagagaga gtagataaaa
    15061 atcccgtgga taatcatcaa aaagtagata accccgagga aaattcccca gttcttcccc
    15121 aggtaatgac tgataatatc ggtgtagtca ttacaggaat cgctttcaga aagggttttt
    15181 aaataaatgt cctgcactac ccaggtggca ggataagcaa tgatcgctgc ggtaataaat
    15241 acccaaatgc ccttcaagcc aatttgtact ggcatcagca cggttccggc accaatcgcc
    15301 atgcctatgc ataataaaac ccagccgaag tcgtagcgtg taaacggcaa tttctgatcg
    15361 tgtttcggta gtgtgttgtg ctgcataaag tcctgtccag aaagaagtcg gcctcgcaaa
    15421 aatgaataaa cccagtttct cttgcgcaac aggtggagaa gtcgaatacg cagtatgtaa
    15481 aaaatgaggc aacagattca ctggggaggt cgcgaggagg cgcaattcta caaaattgcg
    15541 catttcacta ccagtctgga gtcagcccgg atatgaactg caactattgc aaattaagaa
    15601 attaagctgg aaggcatcac gcaatgagga agattgccga gtgctgacag taaaaagcaa
    15661 tcgtaaaatt agcggcaagc cggaagggtt ccggtgaggc gcaatgttgc gggggcttaa
    15721 tccctggtgg cattggttgc tggagagaga aaacccccgc acgttgcagg tatgcacctg
    15781 acaacaccac gggggctaat cttgaaccta gaccactcaa gaatagccgc gaaacgttgt
    15841 cattacaaca caggcggcta tatgacgctc gcagagctgg gcatggcctt ctggcatgat
    15901 ttagcggctc cggtcattgc tggcattctt gccagtatga tcgtgaactg gctgaacaag
    15961 cggaagtaac gtgtcatgcg ggcgtcaggc tgccgtaatg gcaatttgcg cccggaccag
    16021 accgcagggg ggaaactctg cggccttttt cgtgcttact gcgggtaagg cacccagtcg
    16081 ccgccgttca ggcgaacgta cggtttatcc tggtattgaa taactactgc atttgagttc
    16141 tctgagaccg gtgctgtttg tggcaaccca ctggtgagtt ttttccagtc aacattgtct
    16201 tcggtgaaaa tcttgccatc aagaacgcga accaccagat cggagatagc caggaagctg
    16261 ctcggttggt cgatgacaat cggtgccccc tgatgtggtg ccttcatgcc gaagaatttc
    16321 accccaacgg ggacgtcggt gatagacggg ctagggatat cacgtaggcc agatacctgc
    16381 attctgtcgc ccttcagcgc gccgccgtgt tccggcacca cgaccaccat cactttacga
    16441 cccgatttct ccagttcagt aaagaaggcg tccagttcat caaagaattt ctgcgcccgc
    16501 gctttgtaat ccgctgtttt gctgacgccc ggataatggt tgccgtcatg cagtggaagc
    16561 gtgttgtaga acgtggcact acggctgttt ttatcttttt cggtaacgtc cagccagcgg
    16621 ttaagcacgg cggtatcgtc ataaaccggc gaaccatcaa agcccagcaa aataaccggc
    16681 agatttgttt gatccatcaa ttcagtctgc atgccgccat tttcgcgaac ttctttcaaa
    16741 aaaccgccga actggccgtt atgtcccatc atcaggtgct gggtaaagcc cagtttcgac
    16801 aggttatcaa acagatagca gtcgttattt gccggttgat acagattagt gtgcgaagtc
    16861 tgcccgcagc tggcgcgcag taaacggatc gccgccgggc cactgtagga ggtggcggag
    16921 ttaaagttct tgaactcaat atcgaaatgc gaccacagtg gatgcgacat caacccagcg
    16981 gcttctatat ccgaccagga aagcgaacag atgttaatca ccagtagttc aaatggctga
    17041 gcatcagcgg gcagcgaaga cgggaaggtc gatttacgtt tcgcctccgc gttatagaaa
    17101 ttattcagcc aggcgttaag gttcgccgtt gttggcggtg cagtttgtgc gggcatatca
    17161 cccactaccg gtgcgccacc cgtcgccgca acggttgccg ctgcgttacc acccgtcgtt
    17221 gttacagtgg tcgtcggttg tccggctggc cacaaggaga aacttggtcc cgccagggta
    17281 agtacgttca gccatagcag tatggcaacc acaaaaacgg taatgcgaat ccattgtgac
    17341 aggaataacc aggccactaa taaaacaaaa atggccccaa tcatctgcca gttaataaag
    17401 cgtgtgacaa ggtcgattaa ataatcggta ctgaaccccg ccacctgcga accctggctc
    17461 attatgcttt ccgggccagg caaccaggtg tcatgccaga acaaagcaaa gccgatcggc
    17521 agggcaatcc agtggcgcaa gcgatgcagg ctgtagcgcg gaatgggcat cagcagaaac
    17581 gcggcaaaca ccaaattgag gagcggatgg aagttaagat atcccgccca caacaggccg
    17641 aacttaacca gaaaatagaa gttccagccg gaaaggccgc gccagtattg ccagagggaa
    17701 gaaggcatgg cggtattttg cgtaaattga gtcatttttt ggttgccctg gctttttccg
    17761 tgcggcgtaa cgtcccagcc ggtttaacat aacgaggttt agcaaagaac aaacgtaagg
    17821 tgtcgcgaat gcgtcgcaaa gagtgccgcg ccagatagcc cagcgggaaa aatatcagtg
    17881 cgcaaacgac aataatttca atgatatcgc tgatggtcat catgatgagc gctccacagc
    17941 atcatctaac agtcgcatgg gttctggtat tcgtcgccag tggcgaccat cgtgctcggc
    18001 attgatgacc ggtttagaac tttgcgttaa aggcagcggc atgccccatt gttctggggc
    18061 aagcaggcgc atctgcacca gctcggcact gatttgatca tcttcaaacc agaccatacg
    18121 gtttgagaaa atgtcgccag taggcaatgg gaaaatatga ttcaacgcgg tatccagatc
    18181 gttaatccga cagaatgaga gaaacagcac cagccgatta ccgccaatgg tcatgatatc
    18241 gccggtacgg ttagggcgac acagcgtcag ggcttgttca acgcggatac ccggtaccgg
    18301 acgtagggca accagaacgc ctttaccgtg ggcaggtaat agagggttat tcatcatgtt
    18361 gttgacggca ttacagaaca catcccactt ctggaaacca cgcagtttga gcggctgggt
    18421 cattgacagc aaggtagtga tatcttccgg cacatagcga ctaaacttct gcccttgcac
    18481 gctttcgatc atcgtcagac aacgggagag tggcgcattc cacggaataa ccatatttgc
    18541 accgcaggcc aataacaaac gttcatcggt ggcgcgtagg ctagcggtat tttcccgcac
    18601 gaggattttc atcgcactac cgcgctggcg acgcagggta tgaatgctgc gggccagtgg
    18661 ctcgatttgc gcattttgct gtaaagaaaa gaccaccgtc gccgcctgag cggtacgggc
    18721 ttcattgaac aggacttcgt tattgttgaa cagttgccag tgttccgata gcggcggcgc
    18781 accttccagt acagcaacat tactcagaat gcgtttttcg tcgctgcgtg gttggatctc
    18841 cgcctcttcg ctttgaacta atgtccagat accattttgt tgctgaacgc taagctgctg
    18901 acgggcgctg accccttttt cgttgcacca gaaggcaata tccagcaaat gttggtcgcc
    18961 ctgaaaacgc aaactggcaa gaccaaaaag tgaacggtat tcctcaagca acaatgaaaa
    19021 ttgtttatcg ttattatttc cgggattaat taccaaaagc gaacaatggt ttaacctgct
    19081 ccatttattc attttatcca accatgagcg cagccgctcg gcaggaatgt tttgccatgc
    19141 gttatttgcg caaacaagaa taaaaaggta attatgggga tcaatagaat actgcaaatc
    19201 acgggccaaa tagtatagac ctttttcatg atttagcatt gaaaataatt ttattttttc
    19261 cggcccttga gaatcatcta attgaaagat tttcgccgga tcgctgtcca tgctaatgac
    19321 cgcgacgtgt gcggtttcag cctgggatgc aattgtttga ttcgccagac tgatagcatc
    19381 ttcatggcga tcgacgttaa accaccagac gccgcctgct ggcatatgtc gcagctcatc
    19441 ccataatgat gagataccga tagagaatac agggtccaca atgtccctca tgtcaccatt
    19501 ttgtctgatt atcagtttac tagcgaaagc taagaaataa acctaccatt gaaattaaaa
    19561 cttatcagat ttagcatgtg aatcaattta gcaggtcaca agacgtgtga ttttagtctt
    19621 ttcgttttta actttttctg ttgcgtaacg gaaagtcaaa aagtgagcaa attcccgtct
    19681 cgccgtaaac aataaaccgg cgaaatgctc acggttagaa ttgtctcatg aacggtacgg
    19741 ttatttcata gggatcaagc aaaatgaata acaatgaacc agatactctg cctgatcccg
    19801 cgataggcta tatcttccag aatgatattt tggcgttaaa gcaggcattt tcactgcctg
    19861 gtattgatta tgccgatatt tcccaacgcg aacagttggc cgcggcatta aaacgctggc
    19921 cgttgctggc agagtttgcg caacaaaagt aggggattgg tgaatggccg tactgggatt
    19981 gcagggggtg cggggaggcg tggggacaac aaccatcacc gccgcattag cctggtcatt
    20041 acaaatgttg ggagaaaatg tcctggtggt cgatgcctgc ccggacaact tgttgcgcct
    20101 gtcatttaat gttgatttta cccaccgtca gggctgggcc agagctatgc tggatggcta
    20161 ggactggcgc gacgctgggt tgcgctacac ctcgcagctc gatttgctgc cttttggtca
    20221 gttatccatt gaagaacaag aaaatccaca gcactggcaa acccggctga gcgatatttg
    20281 ctccggctta cagcaactaa aagccagcgg gcgttaccag tggattttaa tcgacttacc
    20341 gcgtgatgcc tcgcagataa cccaccagct gctgagtttg tgcgatcact cgctggcaat
    20401 cgtcaatgtg gatgccaact gccatatccg actgcatcag caagcgctgc cggatggcgc
    20461 acatattttg attaataact tccgtattgg cagtcaggtt caggacgata tttaccagct
    20521 ttggttgcaa agccagcgcc gattactgcc gatgctcatt catcgtgatg aagcgatggc
    20581 tgaatgcctg gcggctaagc aaccagtagg tgaatatcgc agtgatgcgc tggcggctga
    20641 agagatattg acgctggcga actggtgcct gttgaactat tccgggctga aaacgccagt
    20701 cgggagtgca tcatgagtat cctgacccgg tggttgctta tcccgccggt caacgcgcgg
    20761 cttatcgggc gttatcgcga ttatcgtcgt cacggtgcgt cggctttcag cgcgacgctc
    20821 ggctgtttct ggatgatcct ggcctggatt tttattccac tggagcaccc gcgctggcag
    20881 cgtattcgcg cagaacataa aaacctgtat ccgcatatca acgcctcgcg tccgcgtccg
    20941 cgtccgctgg acccggtccg ttatctcatt caaacatgct ggttactgat cggtgcatcg
    21001 cgcaaagaaa cgccgaaacc gcgcaggcgg gcattttcag gtctgcagaa tattcgtgga
    21061 cgttaccatc aatggatgaa cgagctgcct gagcgcgtta gccataaaac acagcatctt
    21121 gatgagaaaa aagagctcgg tcatttgagt gccggggcgc ggcggttgat cctcggtatc
    21181 atcgtcacct tctcgctgat tctggcgtta atctgcgtta ctcagccgtt taacccgctg
    21241 gcgcagttta tcttcctgat gctgctgtgg gggggagcgc tgatcgtacg gcggatgccg
    21301 gggcgcttct cagcgctaat gttgattgtg ctgtcgctga ctgtttcttg ccgttatatc
    21361 tggtggcgat atacctctac gctgaactgg gacgatccgg tcagcctggt gtgcgggctt
    21421 attctgctct tcgctgaaac gtacgcgtgg attgtgctgg tgctcggcta cttccaggta
    21481 gtatggccgc tgaatcgtca gccggtgcca ttgccgaaag atatgtcgct gtggccgtcg
    21541 gtggatatct ttgtcccgac ttacaacgaa gatctcaacg tggtgaaaaa taccatttac
    21601 gcctcgctgg gtatcgactg gccgaaagac aagctgaaca tctggatcct cgatgatggc
    21661 ggcagggaag agtttcgcca gtttgcgcaa aacgtggggg tgaagtatat cgcccgtacc
    21721 actcatgaac atgcgaaagc gggcaacatc aacaatgcgc tgaaatatgc caaaggcgag
    21781 ttcgtgtcga ttttcgactg cgaccacgta ccaacgcgat cgttcctgca aatgaccgtg
    21841 ggctggttcc tgaaagagaa acagctggcg atgatgcaga ccccacacca tttcttctcg
    21901 ccggacccgt ttgaacgcaa cctggggcgt tttcgtaaaa caccgaacga aggcacgctg
    21961 ttctatggtc tggtgcagga tggcaacgat atgtgggacg ccactttctt ctgcggttcc
    22021 tgtgcggtga ttcgccgtaa gccgctggat gaaattggcg gcattgctgt cgaaactgtg
    22081 actgaagatg cgcatacttc tctgcgtttg caccgtcgtg gctatacctc cgcgtatatg
    22141 cgtattccgc aggcggcggg gctggcgacc gaaagtctgt cggcgcatat cggtcagcgt
    22201 attcgctggg cgcgcgggat ggtgcaaatc ttccgtctcg ataacccgct caccggtaaa
    22261 gggctgaagt ttgctcagcg gctgtgctac gtcaacgcca tgttccactt cttgtcgggc
    22321 attccacggc tgatcttcct gactgcgccg ctggcgttcc tgctgcttca tgcctacatc
    22381 atctatgcgc cagcgttgat gatcgccctg ttcgtgctgc cgcatatgat ccatgccagc
    22441 ctgaccaact ccaagatcca gggcaaatat cgccactctt tctggagtga aatctacgaa
    22501 acggtgctgg cgtggtatat cgcaccaccg acgctggtgg cgctgattaa cccgcacaaa
    22561 ggcaaattta acgtcaccgc caaaggtgga ctggtggaag aagagtacgt cgactgggtg
    22621 atctcgcggc cctacatctt ccttgttctg ctcaacctgg tgggcgttgc ggtaggcatc
    22681 tggcgctact tctatggccc gccaaccgag atgctcaccg tggtcgtcag tatggtgtgg
    22741 gtattctaca acctgattgt tcttggcggc gcagttgcgg tatcggtaga aagcaaacag
    22801 gtacgccgat cgcaccgcgt ggagatgacg atgcccgcgg caattgcccg cgaagatggt
    22861 cacctcttct cgtgtaccgt tcaggatttc tccgacggtg gtttggggat caagatcaac
    22921 ggtcaggcgc agattctgga agggcagaaa gtgaatctgt tgcttaaacg cggtcagcag
    22981 gaatacgtct tcccgaccca ggtggcgcgc gtgatgggta atgaagttgg gctgaaatta
    23041 atgccgctca ccacccagca acatatcgat tttgtgcagt gtacgtttgc ccgtgcggat
    23101 acatgggcgc tctggcagga cagctacccg gaagataagc cgctggaaag tctgctggat
    23161 attctgaagc tcggcttccg tggctaccgc catctggcgg agtttgcgcc ttcttcggtg
    23221 aagggcatat tccgtgtgct gacttctctg gtttcctggg ttgtatcgtt tattccgcgc
    23281 cgcccggagc ggagcgaaac ggcacaacca tcggatcagg ctttggctca acaatgatga
    23341 taacgcgatg aaaagaaaac tattctggat ttgtgcagtg gctatgggga tgagtgcgtt
    23401 cccctctttc atgacgcagg cgacgccagc aacgcaacca ctgatcaatg ctgagccagc
    23461 tgtagccgcc cagacggaac aaaatccgca ggtggggcaa gtgatgccgg gcgtgcaggg
    23521 cgctgatgcg ccagtcgtgg cgcagaacgg tccttcgcgt gatgtgaagc tgacctttgc
    23581 gcaaattgcg ccgccgccgg gcagcatggt gctacgtggc attaacccga acggcagcat
    23641 tgagtttggt atgcgcagcg atgaagtggt gacgaaggcg atgctcaacc tcgaatacac
    23701 gccatcgcca tcgttactgc ctgtccagtc gcagttaaag gtttatctca atgatgagct
    23761 gatgggcgtg ctgccagtga ccaaagaaca gttgggtaaa aaaacgctgg cgcaaatgcc
    23821 cattaatcca ctgtttatta ccgacttcaa ccgtgtgcgg ctggagtttg tcggccatta
    23881 tcaggacgtg tgcgaaaacc cggccagcac cacgctttgg ctggatgttg ggcggagcag
    23941 tggactggat ctgacctatc agaccctgaa tgtgaagaat gacctgtcac acttcccggt
    24001 gccattcttt gacccgcgtg ataaccgcac caacaccttg ccgatggtct ttgcgggtgc
    24061 gccggatgtt gggctgcaac aagcatctgc cattgtcgcc tcgtggtttg gttcgcgttc
    24121 tggctggcgt gggcagaact tcccggtgct ctataaccaa ctgccggatc gtaatgccat
    24181 tgtctttgca accaacgaca aacggccgga cttcctgcgc gatcatccgg cggtaaaagc
    24241 cccggtgatt gagatgatta accatccgca gaatccttac gtcaaactgc tggtggtgtt
    24301 tggtcgtgac gacaaagacc tgttgcaggc agcgaaaggt atcgctcagg gtaacattct
    24361 gttccgtggt gaaagcgtgg tagtgaatga agtgaaaccg ctgctaccgc gtaagccgta
    24421 cgatgcgccg aactgggtgc gtaccgatcg tccggtcacc tttggtgaac tgaaaaccta
    24481 tgaagaacag ttacaatcca gcggtcttga gccagcagcg attaatgttt cgctaaacct
    24541 gccgccagat ctctacctga tgcgcagtac cggcattgat atggatatta attaccgcta
    24601 caccatgccg ccggtgaaag acagttcgcg gatggatatc agcctgaata accagttcct
    24661 gcaatccttc aacctgagca gcaaacagga ggcgaaccgc ctgctgctgc ggattccggt
    24721 attacaaggt ttgctggatg gcaaaacgga tgtctctatt ccggcgctga aactgggcgc
    24781 gaccaaccag ctgcgcttcg actttgagta tatgaacccg atgcctggcg gttcggtgga
    24841 taactgtatt accttccagc cggtgcagaa tcatgtggtg attggtgacg actccaccat
    24901 cgacttctcg aagtattacc acttcatccc gatgccggat ctacgcgcct ttgctaacgc
    24961 gggcttcccg ttcagccgga tggcggatct gtcgcaaacc atcaccgtga tgccgaaaac
    25021 gcctaacgaa gcacagatgg aaacgttgct gaatactgtt ggttttatcg gcgcacagac
    25081 gggcttcccg gcgattaatc tgacggtgac cgatgatggc agcaccattc agggcaaaga
    25141 tgccgacatc atgatcatcg gtggtatccc ggacaaactg aaagacgata agcagatcga
    25201 cctattggtg caggcgaccg aaagctgggt gaaaacaccg atgcgccaga ccccgttccc
    25261 cggcattgtg ccggacgaga gcgatcgcgc ggcagaaacc cagtcaacgc tgacctcttc
    25321 cggtgcgatg gcggcggtga ttggcttcca gtcgccgtat aacgaccagc gcagcgtgat
    25381 tgcgctgctg gcagatagcc cacgcggtta tgaaatgctt aacgatgcgg tgaacgatag
    25441 cggcaaacgc gccaccatgt tcggttcggt cgcggtgatc cgcgagtccg gtatcaacag
    25501 cctacgtgtt ggcgacgttt attacgtagg tcatctgccg tggttcgagc gcttgtggta
    25561 tgcgctggca aaccatccga ttctgctggc ggtgctggcg gtgctggcgg tgctggcggt
    25621 gctggcggct atcagtgtga tattgctggc atgggtactg tggcgtttgc tgcgtattat
    25681 cagccgtcgt cgtcttaacc cggataacga gtaattgaag atgaatgtgt tgcgtagtgg
    25741 actcgtgacg atgctgctgt tggctgcctt tagtgttcag gcagcctgta cctggcctgc
    25801 ctgggagcag tttaaaaagg attacatcag tcaggaaggg cgcgtcatcg accccagcga
    25861 cgcgcgcaaa atcaccacct ctgaagggca aagttacggc atgttctttg ccctggcggc
    25921 taacgaccgt gcagctttcg ataatattct cgactggacg cagaacaatc tcgctcaggg
    25981 ttctttaaaa gaacgtttgc ccgcctggct gtggggcaag aaagagaaca gtaagtggga
    26041 agtgctggac agcaattcgg cctccgatgg tgatgtctgg atggcttggt cgttgctgga
    26101 ggcggggcgt ttgtggaaag agcagcgtta taccgacatc ggcagcgcgt tgctaaaacg
    26161 tattgcgcgg gaggaagtgg tgacggtgcc tgggctgggt tccatgttgt taccgggcaa
    26221 agtgggtttt gctgaggata acagctggcg ttttaacccc agctacctgc cgccgacgct
    26281 ggcgcagtat ttcacccgct ttggcgcgcc gtggactacg ctgcgcgaaa ccaatcaacg
    26341 tttattgctg gaaaccgccc cgaaaggctt ttcgccagac tgggtgcgct atgagaaaga
    26401 caaaggctgg cagctaaaag ccgaaaaaac attgatcagc agctacgacg ctatccgcgt
    26461 ttacatgtgg gtaggcatga tgcctgacag cgatccgcag aaagcgcgga tgctcaaccg
    26521 gtttaaaccg atggcgacat tcactgagaa aaacggttat ccgccggaaa aagtggatgt
    26581 ggctacgggg aaagcgcagg gtaaaggacc ggtcggtttt tctgccgcca tgctgccctt
    26641 tttacaaaac cgtgatgcgc aggccgttca gcgccagcgc gtggccgata actttcccgg
    26701 cagcgatgcc tattacaact atgtgctgac cctgtttgga caaggctggg atcaacaccg
    26761 tttccgcttc tcgacaaaag gtgagttatt acctgactgg ggccaggaat gcgcaaattc
    26821 acactaaaca tattcacgct ttccctcggt ctggccgtca tgccgatggt cgaggcagca
    26881 ccaaccgctc agcaacagtt gctggagcaa gttcggttag gcgaagcgac ccatcgtgaa
    26941 gacctggtgc agcagtcgtt gtatcggctg gaacttattg atccgaataa cccggacgtc
    27001 gttgccgccc gtttccgttc tttgttacgt cagggcgata ttgatggcgc gcaaaaacag
    27061 ctcgatcggc tgtcgcagtt agcgccgagt tcaaatgcgt ataaatcgtc gcggactacg
    27121 atgctacttt ccacgccgga tggtcgtcag gcactgcaac aggcaagatt gcaggcgact
    27181 accggtcatg cggaagaagc tgtggcgagt tacaacaaac tgttcaacgg tgcgccgccg
    27241 gaaggtgaca ttgctgtcga gtactggagt acggtggcga aaattccggc tcgccgtggc
    27301 gaagcgatta atcagttaaa acgcatcaat gcggatgcac cgggcaatac gggcctgcaa
    27361 aacaatctgg cgctattgct gtttagtagc gatcgccgtg acgaaggttt tgccgtcctg
    27421 gaacagatgg cgaaatcgaa cgccgggcgc gaaggggcct ctaaaatctg gtacgggcag
    27481 attaaagaca tgcccgtcag tgatgccagt gtgtcggcgc tgaaaaaata tctttctatc
    27541 tttagtgatg gcgatagcgt ggcggctgcg caatcgcaac tggcagaaca gcaaaaacag
    27601 ctggccgatc ctgctttccg cgctcgtgcg caaggtttag cggcggtgga ctctggtatg
    27661 gcgggtaaag ccattcccga actacaacag gcagtgcggg caaacccgaa agacagtgaa
    27721 gctctggggg cgctgggcca ggcatattca cagaaaggcg atcgcgccaa tgcagtggcg
    27781 aatctggaaa aagccctcgc actggatccg cacagcagca acaacgacaa atggaacagt
    27841 ctgctgaaag taaaccgcta ctggctggcg atccagcagg gcgatgctgc gctgaaagcc
    27901 aataatcctg accgggcaga acgcctgttc cagcaggcgc gtaatgtcga taacaccgac
    27961 agttatgcag tgctggggct gggcgatgtg gcgatggcgc gaaaagatta tcccgccgcc
    28021 gaacgttatt atcagcagac cttgcgtatg gacagcggca acactaacgc cgtgcgcggg
    28081 ctggcaaata tttaccgcca gcaatcgcca gaaaaagctg aagcgtttat cgcctcactc
    28141 tctgccagtc agcggcgtag cattgatgat atcgaacgca gcctgcaaaa cgaccgtctg
    28201 gcacagcagg cagaggcact ggaaaaccag ggcaaatggg cgcaggcggc agcacttcag
    28261 cggcaacgac tggcgctgga ccccggcagc gtatggatta cttaccgact ttcgcaggat
    28321 ctctggcagg ccggacaacg cagccaggcc gatacgttaa tgcgcaatct ggcgcagcag
    28381 aagccgaacg acccggagca ggtttacgct tacgggctgt acctctctgg tcatgaccag
    28441 gacagagcgg cgctggcgca tatcaacagc ctgccgcgtg cgcagtggag cagcaatatt
    28501 caggagctgg ttaatcgact gcaaagcgat caggtgctgg aaaccgctaa tcgcctgcga
    28561 gaaagcggca aagaagctga agcggaagcg atgctgcgtc agcaaccacc ttccacgcgt
    28621 attgacctca cgctggctga ctgggcgctg cagcgacgtg attacaccgc cgcccgcgct
    28681 gcatatcaga atgtcctgac gcgggagcca actaacgccg atgccattct tggtctgacg
    28741 gaagtggata ttgctgccgg tgacacagcg gcggcacgta gccagctggc gaaactgccc
    28801 gctactgata acgcctcgct gaacacacag cggcgcgtgg cgctggcgca ggcgcagctt
    28861 ggagataccg cagcggcgca gcagacgttt aataagttga tcccgcaggc aaaatctcag
    28921 ccaccgtcga tggaaagcgc gatggtgctg cgcgacggtg cgaagtttga agcgcaggcg
    28981 ggcgatccaa agcaggcgct ggaaacctac aaagacgcca tggtcgtatc cggtgtgact
    29041 acgacgcgtc cgcaggataa cgacaccttt acccgactga cccgtaacga cgagaaagat
    29101 gactggctga aacgcggcgt gcgcagcgat gcggcggacc tctatcgcca gcaggatctt
    29161 aacgtcaccc tcgagcatga ttattggggt tcgagcggca ccggtggtta ctccgatctg
    29221 aaagcgcaca ctaccatgtt gcaggtggat gcgccgtatt ctgacgggcg gatgttcttt
    29281 cgcagtgatt tcgtcaatat gaacgtcggc agtttctcca ctaatgccga tggcaaatgg
    29341 gatgacaact ggggcacctg tacattacgg gactgtagcg gcaaccgcag ccagtcggat
    29401 tccggtgcca gcgtggcggt cggctggcga aatgacgtct ggagctggga tatcggcacc
    29461 acgccgatgg gcttcaacgt ggtggatgtg gtcggcggca tcagttacag cgatgatatc
    29521 gggccgctgg gttacaccgt taacgcccac cgtcgtccca tctccagttc tttgctggcc
    29581 tttggtgggc aaaaagactc cccgagcaat accgggaaaa aatggggcgg cgtgcgtgcc
    29641 gacggcgtgg ggctaagcct gagctacgat aaaggtgaag caaacggcgt ctgggcatcg
    29701 cttagtggcg accagttaac cggtaaaaat gtcgaagata actggcgtgt gcgctggatg
    29761 acgggctatt actataaggt catcaaccag aacaatcgcc gcgtcacaat cggcctgaac
    29821 aacatgatct ggcattacga caaagacctg agtggctact cactcggtta gggcggttac
    29881 tacagcccgc aggaatacct gttgtttgcc ataccggtga tgtggcggga gcgcacggaa
    29941 aactggtcgt gggagctggg ggcgtctggc tcgtggtcgc attcacgcac caaaaccatg
    30001 ccgcgttatc cgctgatgaa tctgatcccg accgactggc aggaagaagc tgcgcggcaa
    30061 tccaacgatg gcggcagcag tcagggcttc ggctatacgg cgcgggcatt acttgaacga
    30121 cgtgttactt ccaactggtt tgtcggcacg gcaattgata tccagcaggc gaaagattac
    30181 gcccccagcc atttcctgct ctacgtacgt tattccgccg ccggatggca gggtgacatg
    30241 gatttaccgc cgcagccgct gataccttac gccgactggt aagttttcag atagcgcctc
    30301 tcttaatgcc gctgcgatcg ggtatactcg ggcggcaatc tgggatttcc ggggggagac
    30361 aatttgcgcg taagtcgctc gttaacaatc aagcagatgg caatggtggc agccgttgtc
    30421 ctggtgttcg tttttatttt ttgcaccgtt ttgctgttcc atctggtcca gcagaatcgc
    30481 tataacacgg ctacgcaact ggaaagcatt gctcgctctg tccgcgaacc tttatcttca
    30541 gccattttga aaggtgatat tcccgaagcg gaagctattc ttgccagcat taaaccggca
    30601 ggcgtggtca gccgtgccga tgtagtgctg cctaaccagt tccaggcgct gcgtaaaagt
    30661 tttattccag agcgtccggt gccggtaatg gttactcgcc tgtttgagct accggttcaa
    30721 atctcgctgg gcgtctactc gctcgaacgt ccggcaaatc cgcagccaat tgcctatctg
    30781 gtgctacagg cggattcctt ccgtatgtat aagttcgtga tgagcaccct ctcaacgtta
    30841 gtgaccattt acttactttt gtcgttaata ttgacggtgg cgattagctg gtgcattaac
    30901 cgcctgattt tgcatccgtt acgcaatatt gctcgcgaac ttaacgccat cccagcccag
    30961 gagcttgttg gtcaccaact ggcattaccg cgtctgcatc aggacgatga aatcggtatg
    31021 ttggtgcgca gttacaacct caaccagcaa ttgctgcagc gccattatga agaacagaac
    31081 gaaaatgcga tgcgcttccc ggtgtcggat ttgccgaaca aagccttgct gatggagatg
    31141 ctggagcagg ttgtcgcgcg taaacaaacc accgcgctga tgatcatcac ctgtgaaact
    31201 ctgcgtgata ctgcgggcgt gctgaaagag gcgcaacgag aaattctgct gctgacgctg
    31261 gtggaaaaac tcaaatcggt actgtcgcca cgtatgatcc tcgcgcagat tagcggttat
    31321 gactttgctg tcattgccaa cggtgtacat gaaccgtggc acgcaatcac tttgggtcag
    31381 caagtgctca ctatcatgag cgagcgcctg ccgattgaac gtattcaact ccgtccgcac
    31441 tgtagcatta gcgtggcgat gttctacggc gatctcaccg ccgaacagct ttacagtcgc
    31501 gctatttctg cggcatttac cgctcgccat aaaggcaaga atcagattca gttctttgat
    31561 ccgcagcaga tggaagccgc ccagaagcgg ttgacggaag agagcgatat ccttaatgca
    31621 ctggaaaatc atcagtttgc tatttggtta cagccacagg tcgagatgac cagcggtaaa
    31681 ctggtcagtg cggaagtgtt actgcgtatc cagcaaccgg atggcagttg ggacctgccg
    31741 gatggcttaa tcgatcgcat tgagtgctgt gggctgatgg ttaccgtcgg tcactgggtg
    31801 ctggaagagt cctgtcgatt gcttgcagcc tggcaagagc gcggcattat gctgcccttg
    31861 tcggtaaacc tctctgtgct gcaactgatg cacccgaata tggtggcgga tatgctggaa
    31921 ctgttaaccc gctatcgcat tcagccggga acactgattc tggaagtgac agaaagccga
    31981 cgtattgacg accctcatgc tgcggtggca atcctccgtc cgctgcgtaa tgccggagtt
    32041 cgggtggcgc tggatgattt cggcatgggc tacgcagggc tgcgtcagct gcagcatatg
    32101 aaatcgttgc caatcgacgt tctgaaaatc gacaaaatgt ttgttgaagg cttgccggaa
    32161 gatagcagca tgattgctgc aattatcatg ctggcgcaga gcctgaactt acaaatgatt
    32221 gccgaaggcg tggagactga agcacaacgc gactggctgg caaaagcggg cgttggtatt
    32281 gcccagggct tcctttttgc tcgtccactc cctattgaaa tcttcgaaga gagttacctg
    32341 gaagaaaagt agctacccca aactgattac aaaactttaa aaagtgctgg tttgtgcgag
    32401 ccagctcaaa ctttttaacc tttttgtttc aattatgatc caggtacatt tctgtgatgt
    32461 tgtctgggtg ttattttaag gccgcaggta ccccataacc ttacaagacc tgtggtttta
    32521 ctaaaggaca ccctatgaaa acctctctgt ttaaaagcct ttactttcag gtcctgacag
    32581 cgatagccat tggtattctc cttggccatt tctatcctga aataggcgag caaatgaaac
    32641 cgcttggcga cggcttcgtt aagctcatta agatgatcat cgctcctgtc atcttttgta
    32701 ccgtcgtaac gggcattgcg ggcatggaaa gcatgaaggc ggtcggtcgt accggcgcag
    32761 tcgcactgct ttactttgaa attgtcagta ccatcgcgct gattattggt cttatcatcg
    32821 ttaacgtcgt gcagcctggt gccggaatga acgtcgatcc ggcaacgctt gatgcgaaag
    32881 cggtagcggt ttacgccgat caggcgaaag accagggcat tgtcgccttc attatggatg
    32941 tcatcccggc gagcgtcatt ggcgcatttg ccagcggtaa cattctgcag gtgctgctgt
    33001 ttgccgtact gtttggtttt gcgctccacc gtctgggcag caaaggccaa ctgattttta
    33061 acgtcatcga aagtttctcg caggtcatct tcggcatcat caatatgatc atgcgtctgg
    33121 cacctattgg tgcgttcggg gcaatggcgt ttaccatcgg taaatacggc gtcggcacac
    33181 tggtgcaact ggggcagctg attatctgtt tctacattac ctgtatcctg tttgtggtgc
    33241 tggtattggg ttcaatcgct aaagcgactg gtttcagtat cttcaaattt atccgctaca
    33301 tccgtgaaga actgctgatt gtactgggga cttcatcttc cgagtcggcg ctgccgcgta
    33361 tgctcgacaa gatggagaaa ctcggctgcc gtaaatcggt ggtggggctg gtcatcccga
    33421 caggctactc gtttaacctt gatggcacat cgatatacct gacaatggcg gcggtgttta
    33481 tcgcccaggc cactaacagt cagatggata tcgtccacca aatcacgctg ttaatcgtgt
    33541 tgctgctttc ttctaaaggg gcggcagggg taacgggtag tggctttatc gtgctggcgg
    33601 cgacgctctc tgcggtgggc catttgccgg tagcgggtct ggcgctgatc ctcggtatcg
    33661 accgctttat gtcagaagct cgtgcgctga ctaacctggt cggtaacggc gtagcgacca
    33721 ttgtcgttgc taagtgggtg aaagaactgg accacaaaaa actggacgat gcgctgaata
    33781 atcgtgcgcc ggatggcaaa acgcacgaat tatcctctta atctcatcac ttatgcccgc
    33841 actcccttcc ccgagtgcgg gtgtttgcgc ataattgacc cctgtgcctg aacttaccac
    33901 ctgggtgagt tcaggtttat ttcacttctt ccgtgacatt ttcatgttct tgcggtctaa
    33961 cacgaagtgt ttttaacgtc atttagactg accgacaacg ggcagttttt ttattaccag
    34021 gatagttgat caggggttca catgcagggc acaaaaattc gacttttagc gggcggtttg
    34081 ctgatgatgg ccactgctgg ctatgtgcag gcagatgcgc tccagcctga tccagcatgg
    34141 caacagggga cgctttccaa cggtttacag tggcaagtgc tgaccacccc ccagcgtccc
    34201 agcgatcgtg ttgaaattcg cctgctggtt aataccggtt cgctcgccga aagtacacaa
    34261 cagagcggtt acagtcacgc catccctcgt attgcgctaa cgcaaagcgg tggccttgac
    34321 gcagcacagg cgcgttcatt gtggcagcag gggatcgacc ctaaacgccc gatgccgccg
    34381 gtaattgtct cttatgacac cacgctgttt aatctgagtt tgcccaataa ccgtaacgat
    34441 ttgctgaaag aggcgctctc ttatctggca aatgccactg gcaaactgac tatcacgcca
    34501 gaaaccatca accacgcgct gcaaagtcag gacatggtgg caacctggcc tgccgatact
    34561 aaagagggct ggtggcgcta tcgtctgaaa gggtcaacct tgttaggtca cgatcctgcc
    34621 gatccgctga aacaacccgt tgaagcggaa aaaattaaag atttctatca gaaatggtac
    34681 accccggatg caatgacgct gctggtggtg ggaaacgtgg atgcgcgctc ggtcgtcgac
    34741 caaatcaaca aaacgtttgg cgaactgaaa ggcaaacgtg aaacaccggc tccggtgccg
    34801 acgctttctc cgctgcgtgc ggaagcggtg agtattatga ctgacgcggt gcgtcaggac
    34861 cggttatcta tcatgtggga tacgccgtgg cagccgattc gtgaatcagc cgcactgctg
    34921 cgctactggc gtgcggacct ggcccgtgag gcgctgttct ggcatgttca gcaagcgtta
    34981 agcgccagta acagcaaaga catcggtctt ggatttgact gccgtgtgct gtatctgcgt
    35041 gcgcagtgtg ccatcaacat cgaatcacca aacgacaagc tgaacagcaa ccttaatctg
    35101 gtggcgcgtg aactggcgaa ggttcgcgat aaaggtctgc cggaagaaga gttcaatgcg
    35161 ttagtggcgc aaaagaaact ggagctgcag aaactgtttg ccgcctatgc acgggctgat
    35221 accgatattc tgatgggcca gcggatgcgt tcgttgcaaa atcaggtcgt cgatatcgcg
    35281 ccggagcagt atcagaaact gcggcaggat ttccttaaca gcctgactgt ggagatgtta
    35341 aatcaggatc tgcgtcagca gttgtcgaat gatatggcgt taatactgct gcagccgaaa
    35401 ggcgagccgg aatttaacat gaaagcgttg caggcggcct gggatcaaat catggcccca
    35461 tctaccgccg ctgcgaccac ctctgtcgcc acggatgacg tacatcctga agtgacggat
    35521 attccacctg cacagtaatg cctgatggtg atgagttagc acaaccatca ggtatttatg
    35581 gatgagctgg atagaggggt aactctttat ccagcctttt gcatatgctg cgtttacgct
    35641 ggcatcgcct cacgcgggat aatcgcgccg cgatactgaa taacggtact tgcggtcagg
    35701 tgcccgcgtt tcgctgcgtt ttccgcactg ccgcccgtca aacgtaccgc cagataaccg
    35761 gcactgaaag agtcgccagc tgcggtggta tcgatcactt tttctttcgg cagtttcacc
    35821 gccggaacat ccactaaccc ttcgccagca atggacacca ggcaagaatc cgccccgcgt
    35881 ttcaccacca cttctttcac gcccgcgtta tgggtgcgcg caatgacgtc ttccaccggc
    35941 tgttgacccc acagcgcgtc ttcatcgtcc agcgtcagga aggcgatatc cgtgcattcc
    36001 agcatttgtt ggtacacctg ctgtgtctct tctttgctgg cccacaggcg cggacgatag
    36061 ttattgtcga aaatcacttt tccgccgttg gcgcggcatt cgcgcagcag ggaaagcagc
    36121 ttttcgcggc tggtcgggct taagatcgcc aggctaatcc cgctcaggta gagataatcg
    36181 aaattcgcca gctcttcgca aatcgccgcg gattgatcgc tctccagcca gaatttggcg
    36241 gcggcttcgt tacgccagta gtagaacgta cgttcgccgg tgctgtcggt ttcaatgtag
    36301 taaaggcccg gcagacggtt ttccatccgt tgggtcaggg aagtatcaac gttctcgccg
    36361 tgccaggcgt ccagcatctg ctggctaaaa ctgtccgttc ccagcgccgt tacgtaatga
    36421 acggttaatg ccgcaggatc gacctgacgg gcgatataga cggaagtgtt cagggtatcg
    36481 ccgccgaaac cgcgcttaac gtccgcgcct ttctcggaaa gctcaatcat gcattcgcca
    36541 atcacggcaa tctttttgga catagtcgtg aacctgatct gtaaaaaatt agcgttagtt
    36601 tgcgctgtgg tggtttgctg gtcaaccata ttaaaacagt gttccattat ttttttgagc
    36661 cagaacgtat tcctgaaaga ttccgttgtg gagtggggaa atttaccccg gccaggcggt
    36721 aaagttctgc ccttgcgcgc cgataatctt tgtcgagtcc gggctgcatc acttttaaac
    36781 acaggacatc tttgatgata aggcaggtta tccagcgaat aagcaaccct gaagcaagca
    36841 tcgagagctt gcaggaacgg cgtttttggt tgcagtgtga gcgtgcttac acctggcagc
    36901 cgatctatca aacatgcggg cggttaatgg ccgtggagct attaacggcg gtcacgcatc
    36961 ccttgaaccc ttcgcaacgc ctgccgccgg atcgctattt tactgaaatc accgtcagcc
    37021 atcggatgga ggttgtgaaa gagcagattg atttgctggc gcaaaaagcc gacttcttta
    37081 tagagcacgg cctgctggca tcggtcaata ttgatggtcc tacgctcatc gccctgcgtc
    37141 agcaaccaaa aatcctgcgc cagattgagc gtcttccctg gctgcgtttc gaactggtgg
    37201 agcatatccg tctgccgaaa gattcaacct ttgcctcgat gtgtgaattt ggcccgctgt
    37261 ggctggatga ttttggtacc gggatggcaa atttctctgc actaagtgaa gtgcgttatg
    37321 actacatcaa aatcgcgcga gaactgtttg tgatgctgcg tcagtcgccg gaaggacgca
    37381 cactcttttc tcagctttta catctaatga atcgctattg tcgcggggtg attgtcgagg
    37441 gcgtagaaac gccggaagag tggcgtgatg tacagaactc gcccgcattc gccgcacaag
    37501 gctggtttct ttcacgcccg gcaccgatag aaacgctgaa tacggcggtt ctggcgctat
    37561 aagctgcctc attttccgcc tggctggact atctttagga ctggcacagg aagaatatga
    37621 ggaagcgaac gatgagcaag gcaggcaaaa taaccgctgc gatttcaggg gctttcttgt
    37681 tgttgattgt cgtggcgatc attttgattg caacttttga ctggaatcga ctcaaaccga
    37741 ccatcaacca gaaagtctct gcggagttga atcgtccgtt cgctatccgt ggcgatctgg
    37801 gcgtggtgtg ggagcggcaa aagcaagaaa ctggctggcg cagctgggtg ccgtggcccc
    37861 atgtacacgc ggaagacatc attcttggca atccaccgga tattcccgaa gtcacgatgg
    37921 tgcatttgcc acgcgtagaa gcaacgctgg ccccgctggc gctgctgacc aaaacggtct
    37981 ggctgccgtg gatcaagctc gaaaagcccg acgcgcgcct gattcgcctc tctgaaaaga
    38041 acaataactg gacgtttaat ctcgccaacg atgataacaa agacgcgaat gcaaagccgt
    38101 cggcatggtc gtttcggctg gataatattc ttttcgatca agggcggatc gccattgatg
    38161 acaaagtaag caaagcggat ctggagattt ttgttgatcc cttaggcaag ccgctgccgt
    38221 tcagcgaagt tactggatcg aaaggtaaag cggataaaga aaaggtgggc gattacgttt
    38281 ttggcctgaa ggcgcaggga cgttataacg gtgaaccgct cacgggtacg ggaaaaatag
    38341 gcggtatgct ggcgctgcgt ggcgaaggga cgccgtttcc ggtacaggct gatttccgtt
    38401 caggtaatac ccgtgttgct tttgatggcg tcgtgaatga cccaatgaag atgggcggtg
    38461 tcgatttacg gcttaaattt tctggcgatt cactgggtga tctctatgaa ctgacgggcg
    38521 ttctgctgcc cgataccccg ccgtttgaaa cggatggtcg gctggtagcg aaaatcgaca
    38581 ctgaaaaatc gtcggtcttt gattatcgcg gctttaatgg gcgaattggc gatagcgata
    38641 tccacggttc tctggtctac accaccggca agccacgacc aaaactggaa ggtgatgtcg
    38701 agtcgcggca attgcggctg gcggacctgg gaccgttgat tggcgttgat tccgggaaag
    38761 gggcagaaaa gtcgaaacgg tctgaacaga agaagggcga aaaaagcgtt cagcctgcgg
    38821 gcaaagtgct gccttatgac cgcttcgaaa ccgataaatg ggacgttatg gatgccgatg
    38881 ttcgcttcaa agggcggcgc attgagcatg gcagtagcct gccgattagc gatctttcta
    38941 ctcatatcat cctcaaaaat gctgacttgc gcctgcaacc gctgaaattt ggcattgcgg
    39001 gtggcagcat tgcggcgaat attcatctgg aaggcgataa aaagccgatg caggggcggg
    39061 cagatattca ggctcgtcga ctgaaactga aagaactgat gcccgatgtg gaactgatgc
    39121 agaagacgct gggggaaatg aacggtgacg cggaactgcg cggtagcggt aactcggtgg
    39181 cggcactttt aggcaacagt aacggcaacc tgaaactgtt gatgaatgac gggctggtga
    39241 gccgcaacct gatggagatt gttgggctga atgtcggcaa ctacattgtc ggtgcgatat
    39301 ttggtgacga tgaggtgcgg gtgaactgcg cggcggcgaa tctgaatatt gccaacggcg
    39361 tggcacgccc gcagattttt gctttcgata ctgagaacgc gttgattaac gttaccggca
    39421 cggcaagttt tgcttcggaa cagctggatt tgactattga tccggagagt aaaggtattc
    39481 ggattatcac actgcgttcg ccgctgtatg tgcgtgggac gtttaaaaat cctcaggctg
    39541 gggtgaaagc cgggccgttg attgcccgtg gtgctgttgc tgcggcactg gcaacgctgg
    39601 ttacgccagc ggcagcgttg ctggcactga tctcaccttc cgaaggggag gctaatcagt
    39661 gccggacgat tttgtcgcag atgaagaagt gaagataatt gccggatgtc tgacatccgg
    39721 caattaaaca gatttagatt gttggcaaag tgtgctttgt tcatgccgga tgcggcgtga
    39781 acgccttatc cggcctacaa atccttgcga attcaatata ttgtatggaa attcaggcct
    39841 gataagcgta gcgcatcagg cttttcactc ttattacaac gactgatgtc gcgtctcatg
    39901 ggtcagcagc agggcgatta acgtcaagcc agccatcgcc gccagatata accccaccgc
    39961 acttagcccg tagttagtct gcaaccaggc tgcgatatat ggcgcaacgg aagccccgag
    40021 aatcgacgct acgttgtaag agaacgatgc tccggtgtaa cgcacttctg tcggaaacag
    40081 ctctggtaac agcgcaccca ttggcccgaa ggtcagaccc atcagactta accccagcag
    40141 caggaaggca aaaaccagaa tcgggttgcc agaaccgagc agtgggttaa aggcgaacag
    40201 cgcgaacagg atgatcagcg tggtgatgat taccatgctt ttacggcgac caaaggcatc
    40261 agccagtaat ccagcgactg gcaccatcac gccaaaacca ataactgcca tcatcaacat
    40321 ccacaacact tcgttacgcg gcaggccaag cccaactggc gcggcggcgg tactaaaggt
    40381 catagagtag accgtcatga tgtaaaacag cgtataggtt gccagcataa tgaacgtacc
    40441 cagtacggtt acgcgaacat gtttggtcag cagcgtaccc agcgggatct tcacctgttt
    40501 tttcgcttta gcgactttct caaacaccgg cgactcatgc agcgacacgc gaacatacag
    40561 gccgataatg accagcaccg ccgagaagat aaatggcaca cgccagcccc agctcataaa
    40621 ctgctcgtcg gtcagtagcc aggaaagcag caagaaagtg ccgttggcaa agaagaagcc
    40681 aatcggtgcg cccagctgcg ggaaggagcc gtacaatgca cgtttgcgcg gtggggcgtt
    40741 ttcagtcgcc agcagcgccg cgccgcccca ttcaccacct aagcccagac cctgaccaaa
    40801 tcgagccagc gccagcagca gcggggcgaa aataccaatc gtggcatagc ctggcagcag
    40861 accaatcacc acggtcgaaa tccccatcgt tagcaacgag gcgaccagcg tcgctttacg
    40921 cccaacgcga tcgccaaaat gaccaaaaac ggcagagcca atggggcgcg cgacgaaggc
    40981 gatggcgaag gtggcgagcg actgtagcgt tgctgccgta ggatcgccct gcgggaagaa
    41041 gatatgcgga aacacaataa cggccgcagt ggcgtaaatg taaaagtcga agaactcaat
    41101 ggccgtgcca atgagagagg cgacaaggac tttattacgc gagttgatcg gcgtgtattc
    41161 ttgctcgtgg tcgagtgttg tggctgttgc ttgcataatc ttttcttatt ttttggctaa
    41221 cgaatagcca tattacgcac agcaaaagtg atatttcaat gtgtaacaaa tcgcggaaat
    41281 ggtcaaaaac caggcaaaaa ggggataatg tttaggccag tgatttttct tctatgaaat
    41341 gcttcacata attttataaa tagtggataa aactggtttc aggttaaata aaagttacca
    41401 actggattta tatgctgggg ttatgagttg gcatgaaatt gtttgctcct gaatagatgg
    41461 ctgaagaggc tctgggaaag agtttcaaga ggttatgtgc gcattatagc cggatgcgat
    41521 gctggcgcat cttattcggc ctacggggag ccaggccgaa taaattcgta tgccggataa
    41581 ggcgttttga actgcatccg gcctatttta aggctgcgtt ttccccggca ttcgcgggtc
    41641 gtctttatat tcggcggtgg caatccacgc cgcgcaaaac agcgtcaaac gggcgaagaa
    41701 gtagaaaaat gccatcagcc ccagcacgga accaaatgct gcgccagagg gggatttcat
    41761 caacgatggc agggtgtagg tcatcacgat tttaatcact tcaaaaccaa tagcagccag
    41821 aaatgttccg cgaatcaggg cttttttgcg cggacggtgg cgcggcaacc gccagaagat
    41881 ccagaagaaa agcagatagt tggcgaagat ggaaatcgcc aggccaatca accgccacgt
    41941 cggtttcagc cactcaatgc tgttcaggtg cagggcgcta ataatcattt gctgcgccga
    42001 accggcaacc gaggtgatcg aaagcgtcac aatcagcgca atcaacaaac caatcagcga
    42061 aataaaatca cgcagatatt ttacccagaa cttctcctga tcttgcggcg agcgttccca
    42121 gacatcgcgc gactgggcac gaatcgcttc acgcaggtta cccatccagt tgatgccgga
    42181 ataaagcgcc accgccaggc cgacaagccc tacagtcgta cgctgctgaa cggcggtgtt
    42241 gatggtgttt ttcaacgtag cggctagcgt cggatcgctg atgttttgca gaattttgtc
    42301 gaagatatcc tgtagcaaca tcggatggga ggccagcaca aagccccctg cggcaaacga
    42361 caccatcaaa atcgggatca tcgacaaaaa tgagaaatag gtgatagccg caccaaactg
    42421 gttacccagc cgatcgttaa agcgttctgt cgcgcgaatc aggtgcgcaa taaccggttg
    42481 gcgttggact ttttcggcag tggtggtgac ggtttccagc gcctggctaa ctttgccgcc
    42541 tttctcgcta ttatccaact gcttaatcgg ctcgtgctcc agatcctggg tgggacgttt
    42601 gatctcgttt tcctgcgtca tccgctcttt tgtccttctg tcgtggtcat tcttaaaaag
    42661 tatagtcggt cagtccaggt actctttcat tacgccagcc agccactgca taaacaggtt
    42721 tacacgccgg gaaagctccc gacgctgcgg ataaaccagc gaaagggaga gcggctcggc
    42781 acggtagcct ggcaacactt caataagccg cccggcacgc agggcttcgc gcacggcaat
    42841 gcgcgggatc tgaataatcc ccagaccggc aagaccggcg gcgagatagt tttcgctact
    42901 gtttaccgtc aacatgccgc cagacttaaa ccactggacg ccattaacgc tggcaacctc
    42961 aaaacctaac ggatgtacac ccaggtgcgg tgtataacgc actattgcgt gtgaagtgag
    43021 atcgtcgggg ttttgcggat aaccaaagcg cgtcagatag tgcggactgg cacagttgac
    43081 catggtcagt ttgccgaggg gacgggcgat aacgccatct tccggtaacg cgccagtgcg
    43141 tatcacgcaa tcaaaaccat catgaagaat atctaccgga cggtcatggc tactcagttc
    43201 cagctcaatt cccggatgct gatagagaaa ttccgacagg tgcggcagta acaggctttt
    43261 tgcgattcct ggcgggatgt cgatgcgtaa tttaccgctg atactggtgg catcctgctg
    43321 aaacagaccg tccagttcgt tgagattact caacacatct tttgctcgtt gataataggt
    43381 catgccttct ggcgtgagtt tgacccgtcg cgtggtgcgc tggagaagct gggtgccaag
    43441 ttgatgttcc agtgcctgta tctggcgcga aacacttcct tttggcaaag caaagaaatc
    43501 cgctgcgcgg gaaaaacttt ccagctccgc gactttgatg aacaactgca ttgcgtgaat
    43561 tttatccatt tctttgaccg attgttgttc acagcgaaac agtaacgcgc atatcgcatt
    43621 gtttattatt ttttctaaca tctaatgccg ggtttcacaa tgttgtcatg acgaatgtgg
    43681 cgatttctca gcaggaaaag ggcggaatag ctttctgaga aaggtgaatc cccttaaaca
    43741 gagcgtattt ttcagcgatt ccctttaaag tgaatagagg acatattggc tatttgtgct
    43801 tatttttgtc tcattgccat ttgttatttt ggcttgtggt cgcggaattt catttttaat
    43861 tgcatgcgta ctaaccacca atataaggaa atcctcaaaa ttgttttaaa tagtgccctc
    43921 gacatgtaat ttcaggcgtt tagctgtaac tatttatatc ggtaactatt ttctacgcgc
    43981 catctctgta gaatggccac cgccaaatgg cgaattctta ttttttctcg cgggtaatac
    44041 ccttccacta cttaagtctt tttaacgtat tcaggtggag attcagttat gcaaatagtc
    44101 atgtttgaca ggcagtcaat atttattcat ggaatgaaaa tcagtttaca gcagcgtatt
    44161 ccaggagtga gtattcaggg ggccagtcag gcagacgagt tatggcaaaa gctggaaagt
    44221 taccctgaag ccttagttat gctcgatggc gatcaggatg gtgagttttg ctactggttg
    44281 ctgcaaaaaa ccgtggtgca atttcctgag gttaaggtgt taattacggc gacggactgc
    44341 aataaacggt ggttacagga agttatccat tttaatgtgc tggccattgt gcctcgtgat
    44401 tcaaccgtcg agacatttgc gttggcggta aatagtgcgg cgatggggat aatgtttctg
    44461 cctggagact ggcggacgac gccggaaaag gacattaaag atctcaaatc gttaagcgcc
    44521 cgccagcggg agattttaac catgttagcg gcgggcgaat caaataagga gatcggcaga
    44581 gcgctgaata tcagtaccgg aacggtaaaa gcgcatctgg aatccctgta tcgccgtctg
    44641 gaagtgaaaa atcgcaccca ggcggcaatg atgttaaata tctcctcctg aagaggataa
    44701 ttttacagcc agcaggaagt tagcggctgg ctgtaaaaat attatggttc gccgtacaaa
    44761 ccaattaaac ggcgtaccac accgttagtc cagccaaacc catcctgcaa cggatactcg
    44821 ccaccgccgc cttcgcgggg aacaccatcg gcaatatggt atttttcgat cagtttgtgc
    44881 tgttccagat aaaactgatt caccgtcttc agccagctgc gcgcgatttc atcgcccaga
    44941 aggtcatcgc catacatttt aaatccctga attgccatcc attgtaacgg tgcccagccg
    45001 ttgggtttat cccactgttc accggtttcg tactcgcttg ccagaatccc gccaggtgtc
    45061 agtaaccgac tgcgcacggc gtttgccaga cgatcggcct gttcatggtt cgccataccg
    45121 acatagagcg gcacaatggc ggcagcggaa aacagcgcca gttgttcgcg tcgccagtcg
    45181 taatcgcggt agatgccgtt ttcatcatcc cagaggtaac ggtttaccgc atcgcgacgg
    45241 gcactggcct tctggcggaa cagtgcttct gtctctttct cacctttcag cgccgagatg
    45301 ttggcgatgg cgctctccag tttaaacagg aaggcattca gatcgatggg gatgaactgg
    45361 gtggtacgaa tgctcgccag acgaccagta tcacggagcc aacgggaaga gtaatcccaa
    45421 ccggaggccg cccccgcgcg taaatcgcgg tacacctcgt tgggcgggcg accagaatgt
    45481 ttcgcggttt caacgtcctc aagccaggat tcgtcacgcg gcgtgtcgcg atcgtcccag
    45541 taacggttga gcagcgatcc gtccggcatc cgcacaacat ggcgataggc ctgattaggg
    45601 atcaacgatt ctgcaccgtc catccagaag gcatattcca ttttcaggtg gtcgagatag
    45661 cggcgcgcac cgcgtacacc atcttcttca aacaactcca ccatcagcgc aaaaaccggt
    45721 ggttgcgagc ggctcaaata ataggtgcgg ttgccgttgg ggatgtgacc gtagttttcg
    45781 atcatccagg cgaagttatc ggccatgcat ttcagcaaat cttcccgacc actttccgcc
    45841 agccccagca tggtgaaata ggaatcccaa tagtaggttt cgctaaaacg gccgcccggg
    45901 acaatatatg actgcggcag cgccagcaga gaagaccacg gaatgtgatc ctgtggttcg
    45961 cgggttagca ccggccacag ctggtcgata tgctctttca gggaattttg cgggtccgat
    46021 acatactcgc tggagtagac ctccggcagc cagaagtgat tttcaacaaa cttgcgcaag
    46081 tcaaaatcac gatgacggcg cactttacgg tagcggatta aaatatccag cgggtccatt
    46141 ttgggtgcgc agtcgggaaa ggttttactg tcggggaaaa ttttcgccga ctgaacatgc
    46201 tcgaacagtt cgagatagcg atcggcaggc gtcagcgcat cagaggcggg cagcccttca
    46261 atcatctcgg gttccggttc tgcctcgatc atctcatcca gttttaattc atacgggtcc
    46321 agttcatagc agagatcgac ctcgatcatc agttcgtctg gattagggtt ttgaattttc
    46381 tgattgagca taaggatcca acctccgaaa ttcgtcgcta attatcgggc ttttctttaa
    46441 gggtagtcat tcgctccgcc tggtgggcga caaagcgtgc ggtggatcac gtttattaac
    46501 ctgtcaaaaa atataacgca ctaatatcaa tggaatttat aaaaatacag atttcgtgtt
    46561 gtttttcatt ttcagaacat tcaatcctgc tcttgccgca atcatgatat gccattcctg
    46621 atctgggtaa taggcacagg ctatcttatt gatagtttat attcatgtaa ttgattgatt
    46681 cctatcaaaa agcgtatctc cctgtttttt atgatggctt tgtctcgcag agacgtttgc
    46741 agcattctgg aaaattttct gggtcacaat aactcaccac cgtgagggaa gaataatgaa
    46801 aatggtctca cgtattaccg cgatcggcct ggctggcgtc gcgatttgct atttagggtt
    46861 atctggttat gtgtggtacc acgataataa acgcagtaaa caggccgatg ttcaggcatc
    46921 tgctgtcagt gaaaataata aggttttagg ctttctccgc gaaaaaggat gcgactattg
    46981 ccacacgcct tcggcagaat tacccgccta ttattatatt cctggcgcga aacagttgat
    47041 ggattacgac attaagcttg gatataaatc ttttaacctc gaggccgtgc gtgctgctct
    47101 gctggctgat aaacccgttt cgcaaagcga tctgaataag attgaatggg tgatgcagta
    47161 tgaaactatg ccgccaacgc gttataccgc gctacactgg gcgggtaagg tgagtgatga
    47221 agagcgggcg gaaatactgg cctggattgc aaaacagcgc gcggaatatt acgccagcaa
    47281 tgatactgct ccggagcatc gcaatgaacc ggtgcagccc atcccgcaaa aactgcctac
    47341 cgatgcgcaa aaagtggcgt tgggctttgc gctgtatcac gatccccgtt tatcggctga
    47401 tagcaccatt tcatgcgctc attgccatgc gttgaatgcg gggggcgtcg atggcagaaa
    47461 aacatcgatt ggtgttggtg gcgcagttgg gccgattaac gcgccgacgg tatttaactc
    47521 agtatttaac gttgagcagt tctgggatgg tcgtgcggca acattgcagg atcaggctgg
    47581 tggaccgccg ttgaacccga ttgaaatggc gtcaaaatcc tgggacgaaa ttattgctaa
    47641 gctggaaaaa gatccgcagc ttaaagcgca gttcctcgac gtctatccgc aaggtttcag
    47701 tggcgaaaat attactgatg ccattgctga atttgagaaa acattaatta cgccggattc
    47761 cccatttgat aaatggttgc gtggggatga aaatgcgctg acggcgcaac agaaaaaagg
    47821 ctatcaatta tttaaagata ataaatgtgc aacttgtcat ggtggaatta ttctcggcgg
    47881 acgttccttc gaaccgttgg ggctgaaaaa agactttaac tttggggaaa ttacggcggc
    47941 ggatattggt cgtatgaatg tgactaaaga agagcgtgat aaattgcgtc agaaagtacc
    48001 cggtttacgt aacgttgctt taacggcacc gtacttccat cgcggtgacg tgccgacgct
    48061 ggacggggcg gtgaaactga tgttgcgcta tcaggtaggc aaagagctgc cgcaggagga
    48121 tgtggatgat atcgtagctt tcctgcacag tctgaacggg gtttacacgc cgtatatgca
    48181 ggataaacaa taattaattt gatcgcccga acaggaatgt ttgggcgatt tttattacga
    48241 taataaagtc tgtttttaat attatcatat taaatgttta tattataaaa gatcattttt
    48301 ctgcttagga ttttgttatt taaattaagc ctgtaatgcc ttgcttccat tgcggataaa
    48361 tcctactttt ttattgcctt caaataaatt taaggagttc gaaatggacc agaagctgtt
    48421 aacggatttc cgctcagaac tactcgattc acgttttggc gcaaaggcca tttctactat
    48481 cgcggagtca aaacgatttc cgctgcacga aatgcgcgat gatgtcgcat tccagattat
    48541 caatgatgaa ttatatcttg atggcaacgc tcgtcagaac ctggccactt tctgccagac
    48601 ctgggacgac gaaaacgtcc ataaattgat ggatttgtcg atcaataaaa actggatcga
    48661 caaagaagaa tatccgcaat ccgcagccat cgacctgcgt tgcgtaaaca tggttgccga
    48721 tctgtggcat gcgcctgcgc cgaaaaatgg tcaggccgtt ggcaccaaca ccattggttc
    48781 ttccgaggct tgtatgcttg gcgggatggc gatgaaatgg cgttggcgca agcgtatgga
    48841 agctgcaggc aaaccaacgg ataaaccaaa cctggtgtgc ggtccggtgc aaatctgctg
    48901 gcataaattc gctcgctact gggatgtgga gctgcgcgag atccctatgc gccccggtca
    48961 gttgtttatg gacccgaaac gcatgattga agcctgcgac gaaaacacca tcggcgtggt
    49021 gccgactttc ggcgtgacct acaccggtaa ctatgagttc ccgcaaccgc tgcacgatgc
    49081 actggataaa ttccaggccg acaccggtat cgacatcgac atgcacatcg acgctgccag
    49141 cggtggcttc ctggcaccgt tcgtcgcccc ggatatcgtc tgggacttcc gcctgccgcg
    49201 tgtgaaatcg atcagtgctt caggccataa attcggtctg gctccgctgg gctgcggctg
    49261 ggtgatctgg cgtgatgaag aagcgctgcc gcaggaactg gtgttcaacg ttgactacct
    49321 cggtggtcag attggtactt tcgccatcaa cttctcccgc ccggcgggtc aggtgattgc
    49381 gcagtattat gaattcctgc gcctcggtcg tgaaggctat accaaagtac agaacgcctc
    49441 ttaccaggtt gccgcttatc tggcggatga aatcgccaaa ctggggccgt atgagttcat
    49501 ctgtacgggt cgtccggacg aaggcatccc ggcggtttgc ttcaaactga aagagggtga
    49561 agatccggga tacaccctgt acgacctctc tgaacgtctg cgtctgcgcg gctggcaggt
    49621 tccggccttc actctcggcg gtgaagccac cgacatcgtg gtgatgcgca ttatgtgtcg
    49681 tcgcggcttc gaaatggact ttgctgaact gttgctggaa gactacaaag cctccctgaa
    49741 atatctcagc gatcacccga aactgcaggg tattgcccag cagaacagct ttaaacacac
    49801 ctgataacat aacgttgtaa aaaccgaatg cccagccttt aaaaaaaaca gctgggcatt
    49861 cggttgctta ttaatcgcaa taatatattg gctgtctatt catcgtgttg ataagatata
    49921 tgttatatga atgtttatta aatgtagcga ttacctttaa tgtcacgaca agggattatt
    49981 tgcttactat taatttccct gcgttcttaa acacgttttt gtattataaa tccagtcatc
    50041 ctgcccgtag aacatcacac attatcatcc tgttctcccg cttcgtttaa atttatttat
    50101 caatcaattt gacttaagag ggcggcgtgc tacattaata aacagtaata tgtttatgta
    50161 atattaagtc aactatgcaa tcattacatg ggaattgtct aattgcgtac gcaagacata
    50221 aatatattct caccatggtt aatggtgaat atcgctattt taatggcggt gacctggttt
    50281 ttgcggatgc aagccaaatt cgagtagata agtgtgttga aaattttgta ttagtatcaa
    50341 gggatacgct ttcattattt ctcccgatgc tcaaggagga ggcattaaat cttcatgcac
    50401 ataaaaaagt ttcttcatta ctcgttcatc actgtagcag agatattcct gtttttcagg
    50461 aagttgcgca actatcgcag aataagaatc ttcgctatgc agaaatgcta cgtaaaagag
    50521 cattaatctt tgcgttgtta tctgtttttc ttgaggatga tcactttata ccgctgcttc
    50581 tgaacgtttt acaaccgaac atgcgaacac gagtttgtac ggttatcaat aataatatcg
    50641 ctcatgagtg gacactagcc cgaatcgcca gcgagctgtt gatgagtcca agtctgttaa
    50701 agaaaaaatt acgcgaagaa gagacatcat attcacagtt gcttactgag tgtagaatgc
    50761 aacgtgcttt gcaacttatt gttatacatg gtttttcaat taagcgagtc gcagtgtcct
    50821 gtggatatca cagcgtgtcg tatttcattt acgtctttcg aaattattat gggatgacgc
    50881 ccacagagta tcaggagcga tcggcgcagg gattgccgaa ccgtgactcg gcggcaagta
    50941 ttgttgcgca agggaatttt tacggcactg actgttctgc ggaaggaata agattataga
    51001 gttttactca gacataaaaa aaacccggca taggggaccg ggaagaggat agtctgccgt
    51061 ctccagacta ataaaccgtt ataacactcc ctgttggcac gggaaacttt gtgctctcag
    51121 taagttaaat ataactttta ctggaaataa gatcagccat ttttttataa acataagcta
    51181 tacgctgtgc gaaaatatat tcgtgttgca tttacttatt atcaattaac tgttatgcaa
    51241 aactactttg tggataaatt ttggtcctac caaatttggc agtttttgcg ctaagaaaca
    51301 gtctggcatc atttcattag tatactgaaa ttgaaataat cgcagtatga aatataaggg
    51361 ataatcatga ctcatgtctg ctcggtgatc ctcattcgtc gttcattcga tatttatcat
    51421 gaacagcaaa aaatatcgct gcataacgag agtatcctgc tgctggagaa aaatttggca
    51481 gacgattttg cgttttgttc accggatacg cgacgactgg atatcgatga gctgacagtt
    51541 tgccattact tacaaaatat tcgtcagcta ccacgcaatt tagggttaca tagcaaagac
    51601 cgtctgttaa ttaaccagtc accccccatg ccgctggtga cggcgatttt tgatagcttc
    51661 aatgaatccg gggtaaattc accgatactg agcaatatgc tctacctttc ctgtttatcg
    51721 atgttttctc ataagaaaga actgatcccc ttacttttca atagcatcag cactgtttca
    51781 ggaaaagttg aacgccttat tagctttgat atcgccaaac gttggtatct gcgcgatatc
    51841 gcggaaagaa tgtataccag cgagagtctc atcaaaaaaa agttgcagga tgaaaacacc
    51901 tgtttcagta aaatattact cgcctccagg atgtcgatgg ccagacgatt actcgagtta
    51961 cgtcaaattc ctctgcatac tattgcggaa aaatgtggct atagcagtac gtcgtacttt
    52021 ataaacacat ttcgacaata ttatggtgta acgccacatc agtttgcgca acattcgcca
    52081 ggtacctttt cctgacatat tttgcatttg aatattggtc aggatctcac acctgcttca
    52141 tgtgaaactc ttccctgatg atttctgccg ggctaccggc tagttctctt tcgcagtttg
    52201 ccaggcggta taggtatttt caggaggaaa agactcggca tgtttgggat tattaagctg
    52261 acaattcata cgattacggg aatgtgggtc agtattgtgc tgttcaaatt gatgacgaat
    52321 ggctggagtg gtttctactt tcagtgctgt gtactaagcc tggtattcct gacggttagc
    52381 tggttgttga gtggtgaatg gttagcagga aagagtaagg ctgaaccttc acgttcaacc
    52441 ttactctcat ttacacgtta cgctttttta aagcgggcaa agagatgttc cactacaacg
    52501 aaaaagaccg gaacaaagta aattgccagc actgttgcgg caaacatccc gcccatcacg
    52561 ccggtaccta ccgcgttttg cgcgccagaa ccggcaccat gactgataac cagcggcagc
    52621 acgccgagaa taaaggccag agaggtcatc aggattgggc gtaaacgcat ccgcgccgct
    52681 tcgatgattg cctctatcgg cgttttccct tctttctgca tcatctcaac ggcaaattcg
    52741 acaatcagga tggcgttctt ggcggaaagc ccgatggtgg tcagcaaacc aacctggaag
    52801 tagacgtcat tacttaagcc gcgcagatcg gtggccagta atgcgccaac gacgcctaac
    52861 ggaacaacca acatcaccga gaacggaatt gaccagctct cataaagtgc ggcgagggcg
    52921 aggaacacca cgaccagtga aatcgcatac agcgcaggag cctgatttga ggataacgct
    52981 tcctgatacg acagtccggt ccatgagtag ccgacgcctg ccggaagttt agcgaccagg
    53041 tctgccataa atttcatggc gtcaccggta cttttcccgg ccgccgcttc acctaaaatc
    53101 tccattgacg ggatgccgtt gtagcgttcc agtcgcggtg aaccataggt ccattcggta
    53161 gacgagtagg cagaaagcgg tgccatcgtg ccagaggcgt tgcgtacata ccattggttg
    53221 atgttatccg gcaacatacg gaacggcgtg cctgcctgga catacacttt tttcacccgc
    53281 ccctggttga ggaagtcgtt cacgtagctg ctgccgaagg cggtggaaat tgtctggttg
    53341 atatcagaca gcgccacacc cattgcctca gctttcgcgg cgttgacgtt cactttgaac
    53401 atcggcgtat cttccaggcc gttcgggcgt accccggtga cctgattcgg tgattgcgct
    53461 gccagtgata acagctcgtt tcgtgcctgg gttagttttt cgtgccccag gttaccgttg
    53521 tccagcagtt ccatatcaaa acctgacgcg gtacccagtt cagccaccgc gggtaagttg
    53581 aacgggaaga cgacggcttt attgatactg cttagcgcaa tcattgcccg ctgaatgatc
    53641 gcggtaaccg agttttcctc accgacacgt tcagaccacg gcttgagact gataaacgcc
    53701 agaccgttgt tttgcccctg gccgctgaag ccaaagccgc caacggtaaa caccgactga
    53761 acattatctt tctcttttgt aagataataa tccgtcacct gttgcagcac tttcgtggtg
    53821 ttaaccatgg tggcaccgga aggtaactgc gcggtggtca taaatacccc ctgatcctct
    53881 tctggtaaga aagaggtcgg cgtgcgcagg aacagcaccg ccatcccggc gcaaatcagc
    53941 aggtagatca ccatgtagcg accggtacaa cgcaacagtg agcgggtgct atcggtatag
    54001 tgctgagttg atttttcaaa cagcgtgttg aagcgtgcga acagggcatt aggtttgtga
    54061 ccgccttccg gcgcggcttt cagaatggtg gcgcacaggg caggggtcag gctcattgcc
    54121 acaaatactg aaagcagcat ggaggagatc agcgtgatgg agaactggcg gtagatctcc
    54181 ccggttgcac cgctcataaa ggccatcggc ataaacactg cggaaagaac aacggcaata
    54241 ccgaccagcg cacgttggat ctgccccatc gatttatgcg tcgcttcctt cggtggtagc
    54301 ttatcttccg caatgacacg ctcgacgttc tccaccacca cgatggcgtc atccaccagt
    54361 aaccctatcg ccagcaccat cccgaacata gtcaacgtgt tgatggtgaa accgaccgcc
    54421 gacaagatcg caaacgtccc gagaataacc accggtacgg caatcgtcgg gatgattgtg
    54481 gcacggaaat tctgcaaaaa cagatacatg accaggaaga cgaggatgat agcctcaacc
    54541 agtgttttga aaacttcctg aatagaaatt tcgataaacg gcgtggtgtc gtaaggataa
    54601 accgtcttca gacttgccgg gaaataggct gataagcggt tcagttcctc tttgactgcc
    54661 cgcgaggtat ccagggcgtt tgctccggta gccagtttga tggcgatccc ggcagcaggt
    54721 ttgccgttat agcgcgccac ggtggaataa tcttccgccc caagttcgac gcgggcgaca
    54781 tcacgcagca gcacttgcga accatcttgc tgaactttca acaggatttt gccaaattct
    54841 tccggcgttt gcagacgcgt ctgcacaatg atcgaggcgt ttagttgctg gtctgccgcc
    54901 tgtggcatgc cacccagttg accaccagaa atctggttgt tttgcacctt aatctgggaa
    54961 ataacatcgg aaggtaccag attgtattta ttgagttttt gcgggtccag ccagatacgc
    55021 atggcgtact cggaaccaaa gagctgtacg ctaccaacgc ccgcggtacg gcttagcggg
    55081 tctttgatat tagacgctac atagtccgcg atatcgtact ggttgaggct gccgttatca
    55141 gaaataaacg ccgctaccat caggatatta ctgctcgatt tatcgacgct aatcccctgc
    55201 tgctgcactg cttcaggtaa tgaaggcata gcgagttgca gtttattttg cacctgaacc
    55261 tgtgcgatat caggagatgt cccagtctcg aaggtcagag tgatagaggc attacccgcc
    55321 gcatcactgg ttgaagacat gtacatcagg ccatcaagcc cattcatatt ttgctcaatc
    55381 acctgagtga ccgagtcttc taccgtttgc gcatcggcac ccggataggt agcgctgacg
    55441 gtaatggtcg gtggcgcaat ctgcggatac tgcgcaaccg gtaagttcat gatcgccaga
    55501 ccacctgcaa gcatcataat aatggcaagt acccaggcaa aaaccgggcg atcaataaaa
    55561 tagttagcca tgaaagtccc cttaagcctg caacgttatt gtttcgattc ggtgctggcg
    55621 ttttcctggc tggaggaaat tgctcgtgct ttgatacccg gacgaatgcg ttgcaaaccg
    55681 gaaacgatca cccgatcgcc agcctgcaag ccagaggtga caacccactg gtcgccgatg
    55741 gctttgctgg cttcaatttc gcgtagcttc acgacatcgt ctttatccag aatgagcgcc
    55801 gttgctttac cttgggcgtt gtgggtgacg ccttcctgcg gcaccagtaa tacattctgg
    55861 cggctacctt catccactaa tgccgtgaca tacatgccag gcagcaagtc accatttggg
    55921 ttggggaaaa tcgcccgtaa cgtcacggag cccgtggttt catcaaccgt cgggtcggag
    55981 aatttcagcg tgccggtctg gctgtaacgt ttaccatttt ccagattgag ctgtactggc
    56041 gtactgccct gaacctgttt gatttgccca ctggcgacct cttctttcat gcgtaagaaa
    56101 tcttgcaccg actgcgtgag atcgacataa atcgggtcca gacgttgtac ggtaaccagc
    56161 gaatctgcct gattagcggt aacgagtgcg ccgacggtca ccgacgattt cccgctgacg
    56221 cccgtaatcg gcgaggtgac attcgcgtat tgcagattga tcgtcgcctg ttcaacagcc
    56281 gctttggcga cggtgacatt ggcttctgct tcattcaact gggtgcgcgc ggtgtcgtaa
    56341 tcctgacggc taacgtagtt ggtcttcagc aacgatgcct ggcggttaaa ggtgatgcgg
    56401 gcattgctgg cggtagagag cgctttcgcc agcgagcctt tggcggagtt tagctcggcc
    56461 tgtaaaggtg caggatcaat ctgatacagc gaatcgccct ggttcacttt atcgccttcg
    56521 ataaagttgc gtttaatgat aataccgccc acctggggac gtatctcggc aacttcataa
    56581 ggaacggttc taccgggcaa ttcgctcaac acattgaccg acccagggga gagtgtgacg
    56641 acaccgacct caggcgtcat ggcggcggtg ttttccgccg atttgtcatc gcaggcggtg
    56701 agcatcgcgc cgcagaataa caacggtatt aacagctttc ttcttctgtt cattttagtc
    56761 cctgaaaatt cttgagaaat aacggttctt taaattgcgc gcgaggagag atatgaggga
    56821 tttacagacc agcggcatag tggggttgtc agccagtaag gtgggataca ggcatagtga
    56881 tcgacatggt gaggtcaacg acaaaaaaaa tgttgccgtt ctgccaacag ttcctgccag
    56941 cattcgggca acgaagggtt ctactggtgg atacacatac caggggaata aagtcgtttt
    57001 agcctctcct ttagtaatca ccgggggtaa tgaaatcagc atctgcatcc ctcgtcatgc
    57061 cagccatcaa tttcagttgc ttatgtcctg actaaaaata agatgtgata cccagggtga
    57121 cgatgtcgct catacgtctt agtttcagct tgtacataat gttgaatttc tgaatcttta
    57181 atgtttgttg cgaaatattg agcgtcgacg tgatattgct ttcattttta tttttcagaa
    57241 tcagttgcat aattttgaac tgattatgtg aaaaacataa ttgtgaattc ttatggggca
    57301 agtgtttacc ataagtaatc tcaagaagat ttttgatact ttctttgcgg ctaacaaaat
    57361 aaataggcgc tttagctttt agtttactga tgtgataagg tgccaaaaca ataattctct
    57421 cggcatctaa tttctccaga aatttaatcg cttcttcatc aaggatatga ttgtgatagg
    57481 tatcaatgat aaccttgaag ggtttattgc ctactttttt gatctctgac agggaattga
    57541 tttttatcat ttcgtgatta tctttcaact gccaaaagcc ctgtaaaaga aaagaatctt
    57601 tcgtcatgag aaaaatcata acttgctcct tagccgttat cgtttcgaac atgtcatcct
    57661 tttcattatt tacatccttg tccgaatcgt tgttcaatat agtaaacgcc tataagaact
    57721 ttgcccttcg tgaactaata cgtctattaa cactaatcaa tacttcagat ttatccatca
    57781 cgcactccat atattgatcc taagtaagat tcctggttgt tatcagcttg taaatagtaa
    57841 acaaaaagtt aacgtttgcg gattactgcc caagaataag ttatcaataa ccagtttgtg
    57901 atctctgaag aatattacta aagttaaaaa gtaatccgat ttaaatatcg agtctccttg
    57961 tttcgactta agctggcaat tggattgcca gctttctttt aacgcattga aattacctga
    58021 gtaaataacg cattcatgtc ttcaggtttt tatatagcgt ctggagacat ggatatttat
    58081 gcaatgtaat caacgccagt tgcaaaaaaa tgaacccgga ttacttattt aaccgcccat
    58141 agcatcaacg cttacggaac tctttgttca ctttcccaca gcacttttaa aacattaaac
    58201 ctgactacgg aaaatatcag ccatgataat gtttgaaatg gctaatttgc catggagtga
    58261 aaaaaattag atgaaattca gtaggttgaa ataatcacta gcaggtaatt atttcaatga
    58321 tagtgcgcaa ttgatctaca acactgcgta gcggagagag tattaatcgg atcatagtca
    58381 catcaagtga ctatgatccg ggtgacaacc gggataatta ttgctgctta acgaacaaac
    58441 tggcgaagct gaacaggctg gcggcgctaa atatcagttc aattcccacc agtgtggaaa
    58501 ccagcgttac agacaccatc ggcgttgcac caaggaatat ccaggcaatg acgatatcca
    58561 gcacaccaat aacgagctgt agccagctgc ctttcattga acgctgacga taccaactca
    58621 tcaggcgaat aacccctgca acacagaaca aaccggcaat aaatgccgca atggcaaaaa
    58681 tgcccagctc cggtgcgcgg atgaagaaat agccgatcaa taaataggcg actgcgacga
    58741 ggaaaccgga taataccggc cagaaattat gactgcggtt gctgaataat ccaacaataa
    58801 gcgcaatgcc cgaacagatt aataatgcac ctactactgt gcttaaaata tcgccagaaa
    58861 cgaacgggaa actgatacac agcaacccga cgataaacag cagcacggca ataaactgga
    58921 ttgctctgcg atgctttttc agcatctcca gatcaaactt caaaattgtt gccttatcta
    58981 tatataacat agaaccaccc tataaaatta agaagaaaat cccctgctat caatctatgc
    59041 caaaaacgcg tctaagaatg cagtcgattt aataaaaatt tcctaattgc agtatctgat
    59101 gcatctgtaa ctcattgtat tgaaataaaa atatctgatt ttgatatttt ccatcaacat
    59161 gacatataca gaaaaccagg ttataacctc agtgtcgaaa ttgattcgtg acggctcttt
    59221 cactttatag ttgaggatat tacgatgaaa aaagtattag gcgttattct tggtggtctg
    59281 cttcttctgc cagttgtgag caatgcagcg gatgcgcaaa aagcagctga taacaaaaaa
    59341 ccggtcaact cctggacctg tgaagatttc ctggctgtgg acgaatcctt ccagccaact
    59401 gcagttggtt ttgctgaagc gctgaacaac aaagataaac cagaagatgc ggttttagat
    59461 gttcagggta ttgcaaccgt aaccccagct atcgttcagg cttgtactca ggataaacaa
    59521 gccaacttta aagataaagt taaaggcgaa tgggacaaaa ttaagaaaga tatgtaattc
    59581 cgggaatgcg ttacatcgta cttccttgca tattgaacag gccggaatat cttctttaaa
    59641 agcagctatt cctcctgttc atatataatc tctatattga atgggttaca aaataaatat
    59701 ttcatctctc cgtaaagcgt ttatttttat gggcgctgta gcggctttgt cactggtgaa
    59761 cgcacaatct gcgctggcag ccaatgaatc cgctaaagat atgacctgcc aggaatttat
    59821 tgatctgaat ccaaaagcaa tgaccccggt tgcatggtgg atgctgcatg aagaaacagt
    59881 atataaaggt ggcgatacag ttactttaaa tgaaaccgat ctcactcaaa ttcctaaagt
    59941 gatcgaatac tgtaagaaaa acccgcagaa aaatttgtat accttcaaaa atcaagcatc
    60001 taatgacttg ccgaattaat gaggtacaag taaaaaggag tggcaagttg agccatcttg
    60061 ctgctccttt ttgcattttt atatgacagc agaatttatt atacgtctta tactcgcggc
    60121 aattgcctgt ggcgctattg gcatggaaag gcaaatgcgc ggcaaaggag cagggttacg
    60181 cactcatgta ttaattggca tgggaagcgc actgtttatg attgtttcga aatatggttt
    60241 tgctgacgtg ctgtctttag atcacgtcgg actggacccc agccgtattg ctgctcaggt
    60301 ggtgacgggc gtcgggttta tcggtgcagg taacattctc gttcgtaacc aaaatattgt
    60361 aggtttgacg acggcagcgg atatctgggt gaccgccgcc ataggtatgg ttattggcag
    60421 tggtatgtac gaactgggta tttatggttc agtgatgacc ttgctggtgc tggaagtctt
    60481 ccatcaatta accttccgcc tgatgaacaa aaattaccat ttacagctga cattagtgaa
    60541 cggtaatacg gtatcaatgc tcgactggtt taagcagcaa aaaataaaaa ctgatttagt
    60601 ttcgttgcag gaaaatgaag atcatgaagt ggtcgccatt gatatccagc ttcatgccac
    60661 aacgtcgata gaagatctgt tgcgattatt aaaaggtatg gcaggggtga aaggcgtttc
    60721 aattagctaa tctgtatagt tatttattcg caggagtacc agtgcggtac tcctgttgct
    60781 tttaatgatc gaggacgatc agtggtgaat ggttggcagg atctttaagt acggtaatat
    60841 ccgcgccgta gagcattgtc agcgcctgct gtgtcaatac gtcctgtggt ttaccatttg
    60901 cgataacctt tcctttttgc attaatacaa cacggtctgc ataatgcgcg gcaagattta
    60961 gatcgtgcaa tatgcagcag acattgaact gccgctcgtg aactaactgg cgtaataaac
    61021 gaaacaagtg ctgctgatgg tgaatatcca gcgccgaagt tggttcatca agaaataacc
    61081 atttcggtga gggagttggt tcccagagtt gcaccagcag gcgcgctaat tgcacgcgct
    61141 gttgttcacc accggataat tgccgatagt cgcggtttgc taacgcctga cagtcacata
    61201 acgccattat ttgtgctgtt tcattatcct gattaccagt acgatgcgga tgccgaccca
    61261 tctggataac ttcttgtacg ctgaatggaa acgccatgtg gctattttga cgcatcacgg
    61321 cccggtgttt cgccagttca gtaatcgacc attcatttaa tggcttatta aacaaccgac
    61381 attcgccgct atcaggttga agatagccgg ttaactggcg aagcagcgta gattttcccg
    61441 caccattagg gccgagaatg gcaacaattt ccccaccggg aaaagtcagc gagacattat
    61501 cagtcagacg ccgcccctga aggctataaa ccagattctg ggcgctgatc atgtgatcct
    61561 ccgatttcgc agaatcaacc acataaaata agggccacca agcaggctgg ttaataatcc
    61621 cacgggcatt tctgctggct gtaccagcgt gcgggcaagc gtgtctgcca tcagcaataa
    61681 acaggctccg gcgagggcgg aacaagggat gagccagcgg tgatttgctc cggtagtcat
    61741 gcgaatcaga tgcgggatca ccaggccaat aaagccgata atgccgctta ccgatacggc
    61801 agcaccaacg agcagtgagc tcactaatag taattgctgg cgtttacgct taacgttcac
    61861 gccgaggtaa tgggcttctt catcaccgag ctgcagtaaa ttcagcgttc cggcgagaca
    61921 agttgttgcg ataatggccg gaaggatgaa tgaactggca accaataacg tcggccattg
    61981 cgcctgtcca agattgccca tcatccacaa tgtgagttgt cgtagctgct gctcatcgcc
    62041 aatatagctc aggatgccga tcgctgcgcc gcacagggca ttgatggcga taccggcgag
    62101 taataattgc atcatgctgc catcgcgatg gcgctgcgtg atgagaaaaa tgatggcgca
    62161 gactactaaa cttccggcaa tggcaaacac catttgctca tagagcacca gcaccactgg
    62221 gaaggagaag ggcagcacaa ttgcaacgcc aaccattaat gcagaaccgc tactgacgcc
    62281 gagtaatcct gggtcagcca ttgggttgcg aaaaagcccc tgcataatta cccctgcggt
    62341 tgccaacgct gcacctacca gcaccgccag caaaacccgt ggtagacgaa tattgagcca
    62401 gatatcgcgg tattcctcat caaaaagggc atcaaaactg atctttaacg ctccctgact
    62461 agcaccgaac agcaccaggg caagcaataa tagtgatagc cccataaaga ccctggcgga
    62521 tgaaaatgaa tccttgagca cgcaaaactc cgttacgaca ttacggcgcg gacatgccgc
    62581 gccggggtat cacgccggtt tcaggtccgg ttcaatcaga ctgtagacct gctggttaag
    62641 tgctggcgca tttgccagtt catggatatg tgcggcgaca tcggcacggc ggacaaaacc
    62701 atgacactcc tgattttgta tccgttgtgc tttgcctgtt gctgcgccgt caagcagacc
    62761 accagggcgg agaatggcgt agtctagctg gctggtttgc agccagcttt ccgccagcgt
    62821 tttttcccgt accgcctgac caaacgcagc ctttgcccgc tccgataaaa acggccagct
    62881 atcgccgcat cccaaagagg tcaccagaat catgcgactg atgccggcct tttcggcctc
    62941 atcaatcact gttctgtgcg ccagataatc ctgagctcct cccattgttg agatgataag
    63001 cgcatccggg ccagctgcgc ggcaagcagc cgcgactacg ctggcatcac aggcgtcgcc
    63061 tgtaaaaacc tgtacgcctt gttgcgccag tttcgtggcg gcatcggcat gacgaatgac
    63121 agcaacgacc ggacgttgtt ccgccagcgc cagttcaagc gttctggcac ccacaccttt
    63181 tccgcctgcg ccaaagagta gccacggcgt catcaggcgt gttcctttaa cgatgccgcc
    63241 agagtatgga aggcgctgac ttgttcgctc aggagctgac gatggtcatc cctgccgagg
    63301 aagattttca gcatggcgct gccttctttg ttgaaaaaga gaatggacgc ggtgtccatg
    63361 cccataaatt tacgttcgat taaggcaata tgcgtgcagt tttctgcttt gatatgcccg
    63421 gacatgccgt gctttccgcg taggttgaaa tagccgtggc gatggaaacc cgaaggcagt
    63481 tcgccgctaa attcgaggat tacatcggca gtatgtacta acgtggtgac gttgccccat
    63541 tcgcagacgg tatcccagac agtatcgaat ttatcgcctg gcactacggt agaggagggt
    63601 agatttctca ctacctccag cagtgtggtg ttgtattgtt cggctaccac ctccagcgta
    63661 ccgtccggct ccgttttcaa aaattcttgt aacgagacat ggctcataaa tcactcctgt
    63721 tatggttttt cacgcataat cgctggcgca ttaagtacct gaattagttc attaagagac
    63781 tgcaaaatat tgcttgccca gaaacgacct tcattagtca gccgcaggca agttgaagca
    63841 tcgcgactta agcctttttg gtgccattga gccagtaacg gcgcgagttt ttcggcatgt
    63901 ggcgtaagtt catccagcgg tacgcgcgct gtttcaacac ctgactgcaa ggtatgacgc
    63961 cattgcgcat tacgttcggc gttacgcatg atcaacatca gcggtttttt gcctgccgcg
    64021 acggattcat gccaggtctg aagattgcgt tcgttcatcc aggagtaacc attaatcgaa
    64081 ccgccggctc cggaaccaaa ggccagacaa tcggcacctt gtttaatcag caggttatag
    64141 agattgcgtt cgcgtgtggt acggccccag tggctgttac tgatgcagcg ccaaccggca
    64201 tcgtccataa aatcacatcc ttgcagataa agatcgcgac gttctgccgg tgagggtacg
    64261 gtagtacgcc cattttccac ggccttgccc agcggtgtat tggacaggac attgagcgca
    64321 tagagatcga cgccgtcgag accgatatcg cgggcaatag ccagatcttc tccccaggtt
    64381 tgcgcatcct gacccggcag gccaaacagc aggtcacaga ccaccgcagc acggtcacgt
    64441 ttgaccaggc tttccataaa cgcaatagcg gttgggccat ccgaggtgcg ggccattttc
    64501 ttgcggattt tgctgttaaa cgactggata ccaattgaga agcggtttgc tcctgcatca
    64561 aggcaagcat cgattcgctc agcgtcaaaa ttcaataccc gtccttcgat agtgatttca
    64621 caatcagggg ccagtggcag cttttctcgt aatgtggtga tgattctggc caaatcgtgt
    64681 gccgaaagtg cggaaggcgt accgccaccg aaatagaccg cgtggatggg ggcagactga
    64741 tgcaatacgc tgtccgcttc catttctatt tcacgaatca gggcgtcggt ataatgagca
    64801 cacgcatctt cattaaaacg gttctgataa aaaccacaaa acgtgcagtg tgtcgcacaa
    64861 aaaggaatgt gtaggtaaac cagtcgctta cgaggtgatg ccgtttgatt gatcatctct
    64921 tgccaggtct gtgccagttg ctctttggca accggaatgg ctccacgaaa cggcatcatg
    64981 gcgcgcctgt ctttaaatgg ctgatcaccg tctaaagcaa aatgtggcgt gagatccagg
    65041 gtgttatttg cgttcattcg tctgatttaa atccatttat tgcggccaaa gctgctggtg
    65101 taaagactca acgacatcag caatacgcgg acccattccc agaatcaggt tttgatcaac
    65161 ggtgataatg cgctggtttt tccatgcggc agtgtgggta attccagcaa tagaacgtag
    65221 ccgattaata tcgccatcga ccatttgtga ggttacgaca atcacttctg gattagccgc
    65281 aatcagtgat tctgcactgt agcttttgta ctgctggtgg gtagcgacgt tttctgctcc
    65341 ggcaagcgaa agaatggcgt ccgcgacgct gcctttaccg gcaacttgtg gcgcacttcc
    65401 gcctgcggag agaataaaca tggctttaac cggggctttt ttggtcgcca cgttttgctg
    65461 tactcgctcc aggcgttggc tgatttgtgt caccagcgcc tcgccttgtt caggaacctg
    65521 tagcgttttt gccagttggc gaatgttggc gtacatctgc tgaagggtgg caggcacacg
    65581 cggcagggtg acgacgttga ctttctgcgc tcgtagttgg tcgagcacaa tttgtggccc
    65641 ggcatcctgc caggtaatta cgctatccgg gcgaagtgac aaaatgcctt cgctgctgag
    65701 ttgtttccag tagccaatat gcggcagttt ggcggtttct ggtggataag atgtcgtttc
    65761 atcgacacca accacgcgtt cgccagcgcc catcgcgtag attagctccg tcagcgatcc
    65821 tcctgcgacc acgatacgtt cggcagccgt tacgctaaaa gcccagctaa gagccagtac
    65881 tgccgtaaac aattttttac gttttactgc ggtcttcgcg gcagaggcca cggctgaaat
    65941 acataacgtc agtggggaaa aaagaaacgg cctggtgatg atccttggca taatatgaga
    66001 actcttatca aattgagaat gatcatgatt atcatttgag ttatatatga aatacaaccg
    66061 tcgattcaat acgcaaggcg ttacaagcgt atttattttt gatttttatc aagatgcttc
    66121 gtcagctaac gttcttgttt ccgactaaat ttcatcgcgg gaaaaagctg agaacccaga
    66181 gatatcgagg cttgcagagc aaagagaaga aaaaattatc actgttaacg acaaaattta
    66241 gtgatgaggc atggggggat aaccataaaa acaaggtggt ttacagggcg caggataggt
    66301 ttcataaccc cctgtttatt gtgttaatgg tggttaaaaa aaaacttgat cataaaaaat
    66361 tgatcgataa taattctcat tatcacgttt gcatttaaca aaacacgacc atatggataa
    66421 caaaaccata gcatttgcaa gggataacgc acagctctct tcactcctga tggggcatat
    66481 cgttttgcct gacaaagagg gcgtgctttt gcgtaattgc cgagttatca ggcaatttca
    66541 tgggatataa acgcttgtaa actcacccgg cgaatattat cgctctgaat tttaaataaa
    66601 tttatttttc gccagccacc ccggtattaa cccaggcagc cagcattttt tattttcctc
    66661 atggagaatc gctatgtcac gtccgcaatt tacctcgttg cgtttgagtt tattggcctt
    66721 agctgtttct gccaccttgc caacgtttgc ttttgctact gaaaccatga ccgttacggc
    66781 aacggggaat gcccgtagtt ccttcgaagc gcctatgatg gtcagcgtca tcgacacttc
    66841 cgctcctgaa aatcaaacgg ctacttcagc caccgatctg ctgcgtcatg ttcctggaat
    66901 tactctggat ggtaccggac gaaccaacgg tcaggatgta aatatgcgtg gctatgatca
    66961 tcgcggcgtg ctggttcttg tcgatggtgt tcgtcaggga acggataccg gacacctgaa
    67021 tggcactttt ctcgatccgg cgctgatcaa gcgtgttgag attgttcgtg gaccttcagc
    67081 attactgtat ggcagtggcg cgctgggtgg agtgatctcc tacgatacgg tcgatgcaaa
    67141 agatttattg caggaaggac aaagcagtgg ttttcgtgtc tttggtactg gcggcacggg
    67201 ggaccatagc ctgggattag gcgcgagcgc gtttgggcga actgaaaatc tggatggtat
    67261 tgtggcctgg tccagtcgcg atcggggtga tttacgccag agcaatggtg aaaccgcgcc
    67321 gaatgacgag tccattaata acatgctggc gaaagggacc tggcaaattg attcagccca
    67381 gtctctgagc ggtttagtgc gttactacaa caacgacgcg cgtgaaccaa aaaatccgca
    67441 gaccgttgag gcttctgaaa gcagcaaccc gatggttgat cgttcaacaa ttcaacgcga
    67501 tgcgcagctt tcttataaac tcgccccgca gggcaacgac tggttaaatg cagatgcaaa
    67561 aatttattgg tcggaagtcc gtattaatgc gcaaaacacg gggagttccg gcgagtatcg
    67621 tgaacagata acaaaaggag ccaggctgga gaaccgttcc actctctttg ccgacagttt
    67681 cgcttctcac ttactgacat atggcggtga gtattatcgt caggaacaac atccgggcgg
    67741 cgcgacgacg ggcttcccgc aagcaaaaat cgattttagc tccggctggc tacaggatga
    67801 gatcacctta cgcgatctgc cgattaccct gcttggcgga acccgctatg acagttatcg
    67861 cggtagcagt gacggttaca aagatgttga tgccgacaaa tggtcatctc gtgcggggat
    67921 gactatcaat ccgactaact ggctgatgtt atttggctca tatgcccagg cattccgcgc
    67981 cccgacgatg ggcgaaatgt ataacgattc taagcacttc tcgattggtc gcttctatac
    68041 caactattgg gtgccaaacc cgaacttacg tccggaaact aacgaaactc aggagtacgg
    68101 ttttgggctg cgttttgatg acctgatgtt gtccaatgat gctctggaat ttaaagccag
    68161 ctactttgat accaaagcga aggattacat ctccacgacc gtcgatttcg cggcggcgac
    68221 gactatgtcg tataacgtcc cgaacgccaa aatctggggc tgggatgtga tgacgaaata
    68281 taccactgat ctgtttagcc ttgatgtggc ctataaccgt acccgcggca aagacaccga
    68341 taccggcgaa tacatctcca gcattaaccc ggatactgtt accagcactc tgaatattcc
    68401 gatcgctcac agtggcttct ctgttgggtg ggttggtacg tttgccgatc gctcaacaca
    68461 tatcagcagc agttacagca aacaaccagg ctatggcgtg aatgatttct acgtcagtta
    68521 tcaaggacaa caggcgctca aaggtatgac cactactttg gtgttgggta acgctttcga
    68581 caaagagtac tggtcgccgc aaggcatccc acaggatggt cgtaacggaa aaattttcgt
    68641 gagttatcaa tggtaatcat ctgccccgat atttcggggc atttatctgg aaggaagaga
    68701 gacaatgaac cactacacac gctggcttga gttaaaagaa caaaatcccg gaaagtacgc
    68761 gcgtgacatc gcagggttaa tgaatattag agaagcagaa ctggcatttg cacgcgtcac
    68821 gcacgatgcg tggcggatgc acggcgatat ccgtgaaatt ctggcggcgc tcgaaagtgt
    68881 tggcgaaacc aaatgtattt gtcgtaatga atatgcagtc catgagcaag ttggtacgtt
    68941 cacaaaccag catttgaacg gacatgccgg attgatcctc aatccgcgcg cgctggattt
    69001 acgtctgttt ctcaatcaat gggccagtgt tttccacatc aaagaaaaca cggctcgtgg
    69061 cgaacgccag agtattcagt tctttgatca tcagggcgat gcattactaa aagtttatgc
    69121 caccgacaat accgatatgg cggcatggag tgagcttctg gcacggttta tcaccgatga
    69181 gaatacgccg cttgagttaa aagccgttga tgcgccagtt gttcaaacgc gagccgatgc
    69241 cactgtggtc gagcaagagt ggcgggcgat gaccgacgtt catcagtttt ttacgttgct
    69301 caagcgccac aacctgacgc gccaacaggc gttcaatctg gtggcagacg atttggcctg
    69361 caaagtatcc aacagtgcgt tggcgcaaat tcttgaatct gcacagcagg atggtaatga
    69421 aatcatggtg tttgttggca accgtggctg cgtacagatt ttcaccggtg tggtagaaaa
    69481 agtggtgcca atgaaaggtt ggctgaatat tttcaacccg acgtttactc ttcatctatt
    69541 agaagagagc attgctgaag cctgggttac ccgtaaaccg accagcgatg gctacgtaac
    69601 cagtctggaa ttgtttgccc atgatggtac gcagatagcg caactttatg gtcaacgtac
    69661 agaaggcgaa caggagcaag cgcaatggcg taagcaaatt gcttcgctga taccggaagg
    69721 cgttgctgca taacgtataa ttaacgggag gtctggctat ttattgctgg gcctctttat
    69781 attgatcaat cgttcacacc agataatcaa tatgctgatg gcgtaccagc tcattgatcc
    69841 gcttaagatt gaggatcatc atgatattgt gtttatggca gtagaacgtt tttagcgata
    69901 ttttcagttt ctccagaata tcttccgttg tcatttgatt cgcaatgcag ctcagaacct
    69961 gatgttgctt gcgcgaaagt gacacattcg cttcaggcac agagcagtaa ctgccattga
    70021 tgagtgcgac aaaatcaatg atagtgatag tccgcggaac aaaggtcacc ggcactttcc
    70081 ccaggcagcg tttaatatta aagggggaga aaataatgac atggacaggt tttaatcgtt
    70141 caatttgagt gagaaaatta gaatgaaata cattattcat taatgtatca ataatgacga
    70201 aggcattctg atgcaacatt acgtcgatct cttcttcgtt ctgtatatga acgacattac
    70261 ctttactcag aatgtttttc atcgcggtga agaacatcgt atccctggta attataagaa
    70321 acataaccga ctcctggtgc ggacattaac tttataatca gtttgccaaa ggtaattaac
    70381 aggacagggc ttataataat aatagttagc ttactgtgta ttaatttgat gtaatgcatg
    70441 atgcagatgc tgagaaagca tctgcatctt tcggtggtgt tatttgacca gctcaggtgt
    70501 tacctgactc accgcattgg tgtagtaagg cgcaccccag cccggttccg gccagaatgc
    70561 accatagccg taatcccaca ggttataggt ggtattaacc acttctctca aatgccacac
    70621 ctggatgccc tgcatattca cttccaggaa gttatacggg actttattga taaagccagg
    70681 ttgttcaccc tgaatggtgc cgaggatggt aacaaagtga ttacgatagt tcactggatc
    70741 aaggaagccg ctttgtctgg cgagcagtcg gccctgatag ttggcttcaa tatcaggctt
    70801 cgcatagcta tccaacggta atacagcgat ttctaacaac gtatccgttt tgccattgat
    70861 aacgttgata accttcccac caaagcgcgc ttgttgacca acatataacc ccggctggtt
    70921 atgaacagca acaaaacttt tttgaatatc aggttggtta tttcctttga tattttgcgg
    70981 aattgaacta catgctgcaa gcaaaaatga aaggctgagt atgagtgcac cttttgtcat
    71041 gttcatgtta ctatccttat caacaaatat taaaaccata atagatattt ctttatagtt
    71101 ttcatctgat tctgaggcga tatgcctgat agtaacgtaa acctgtctga tgtttatatc
    71161 ccttctgttt agttcaattc cgttattttg gggcgtggca tgagtatcca tcgccccata
    71221 atataacc
//
LOCUS       DAZFAB010000012        69272 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZFAB010000012 DAZFAB010000000
VERSION     DAZFAB010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 69272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.012"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(156..2570)
                     /gene="ybbP"
                     /locus_tag="ACHJWA_000949"
     CDS             complement(156..2570)
                     /gene="ybbP"
                     /locus_tag="ACHJWA_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFO4307596.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGSENQLDSYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSVFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVTPLKAF
                     LAEHQIVPESFYPVVRARLTAINGKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVTLGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
     gene            complement(2567..3253)
                     /gene="ybbA"
                     /locus_tag="ACHJWA_000950"
     CDS             complement(2567..3253)
                     /gene="ybbA"
                     /locus_tag="ACHJWA_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFO4307597.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            3224..3847
                     /gene="tesA"
                     /locus_tag="ACHJWA_000951"
     CDS             3224..3847
                     /gene="tesA"
                     /locus_tag="ACHJWA_000951"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFO4307598.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFLMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            3837..4646
                     /gene="ybbO"
                     /locus_tag="ACHJWA_000952"
     CDS             3837..4646
                     /gene="ybbO"
                     /locus_tag="ACHJWA_000952"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO4307599.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRRSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            4707..5561
                     /gene="cnoX"
                     /locus_tag="ACHJWA_000953"
     CDS             4707..5561
                     /gene="cnoX"
                     /locus_tag="ACHJWA_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFO4307600.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(5624..6403)
                     /gene="fetB"
                     /locus_tag="ACHJWA_000954"
     CDS             complement(5624..6403)
                     /gene="fetB"
                     /locus_tag="ACHJWA_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFO4307601.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(6390..7067)
                     /gene="fetA"
                     /locus_tag="ACHJWA_000955"
     CDS             complement(6390..7067)
                     /gene="fetA"
                     /locus_tag="ACHJWA_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFO4307602.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            7213..8130
                     /gene="qmcA"
                     /locus_tag="ACHJWA_000956"
     CDS             7213..8130
                     /gene="qmcA"
                     /locus_tag="ACHJWA_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFO4307603.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            8127..8585
                     /gene="ybbJ"
                     /locus_tag="ACHJWA_000957"
     CDS             8127..8585
                     /gene="ybbJ"
                     /locus_tag="ACHJWA_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFO4307604.1"
                     /translation="MMELIVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAVLTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLIIRAVIA"
     gene            complement(8764..9126)
                     /locus_tag="ACHJWA_000958"
     CDS             complement(8764..9126)
                     /locus_tag="ACHJWA_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4307605.1"
                     /translation="MNEFEKIFNEMNLDRALLPILFRSNRSTVWKYLSGDSTAPASAM
                     SLIMLLQLIQKRNPDLLAEWLTLSDFTIPPEVYLDQPDYWKGWVYTQHKVNKNVLEYL
                     KKHYPDEDQKSMSKGREE"
     gene            9493..10509
                     /locus_tag="ACHJWA_000959"
     CDS             9493..10509
                     /locus_tag="ACHJWA_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO4307606.1"
                     /translation="MKTVNVALLALIISATSSPVVLAGDTIEAAATELSAINSGMSQS
                     EIEQKITRFLERTDNSPAAYTYLTEHHYIPSETPDTTQTPTVQTDPDAGQKTVAATGD
                     VQTTARYQSMINARQSAVTDAQQTQITEQQAQIVATQKTLAATGDTQNTAHYQEMINA
                     RLAAQNEANQRTATEQGQKMNALTTDVAVQQQNERTQYDKQMQSLAQESAQAHEQIDS
                     LSQDVTQTHQQLTNTQKRVADNSQQINTLNNHFSSLKNEVDDNRKEANAGTASAIAIA
                     SQPQVKTGDVMMVSAGAGTFNGESAVSVGTSFNAGTHTVLKAGISADTQSDFGAGVGV
                     GYSF"
     gene            10594..11016
                     /locus_tag="ACHJWA_000960"
     CDS             10594..11016
                     /locus_tag="ACHJWA_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO4307607.1"
                     /translation="MMNSSIKSFSLLAVILLAGCSSPTSRIADCQAQGVSHDTCYLAE
                     QQRQAAILSASEAQAFKNAEAAQHAQAAKKAIYKGFGMTFRMSSKNFAYLNDSLCAID
                     EDNKDATVYQSGLYNVIVYHHTGKVALMKEGQFVGYLK"
     gene            11013..11216
                     /locus_tag="ACHJWA_000961"
     CDS             11013..11216
                     /locus_tag="ACHJWA_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4307608.1"
                     /translation="MKEQRKIPLTHIMIIGAFIFAFLQVVLLASLVHAVNVNNEIQEG
                     LFQSGRIMVESLQHILSVQTGIH"
     gene            complement(11220..11627)
                     /gene="cueR"
                     /locus_tag="ACHJWA_000962"
     CDS             complement(11220..11627)
                     /gene="cueR"
                     /locus_tag="ACHJWA_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFO4307609.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     NQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(11624..12799)
                     /locus_tag="ACHJWA_000963"
     CDS             complement(11624..12799)
                     /locus_tag="ACHJWA_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HFO4307610.1"
                     /translation="MNGQLQYNRGLTEPRLPRSALAVRVTAVMLLCFLGWAWYFQLDE
                     VTTGSGTVEPSGREQVVQSLEGGILYHLDVKVGDIVEQGQPLAQLNRTKTESDVQEAM
                     SRLYAALATSARLRAEVSNKPLVFPDELNKFPELIESETALYNTRRDGLNKATTGLTQ
                     GISLVNRELAMTQPLVKQGAASSVEVLRLQRQANELENKLSDVRTQYYVQAREELAKA
                     NAEVETQRSVIRGREDSLTRLNFTAPVRGIVQDIDVTTVGGVIAPGGKLMTIVPLDEQ
                     LLIEAKISPRDVAFIHPGQKSLVKITAYDYSIYGGLPGEVAVISPDTVQDEVRRDVYY
                     YRVYIRTFSNHLENKSKQQFPIFPGMVATVDIRTGKKSVLDYLLKPFNKAQEALRER"
     gene            complement(12796..14958)
                     /locus_tag="ACHJWA_000964"
     CDS             complement(12796..14958)
                     /locus_tag="ACHJWA_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HFO4307611.1"
                     /translation="MKKNAQSVEAWLEAMIAVARYYRLDFSQENVRATVNWERDSKRE
                     ELLTDMARQLGMGLRLVEFSADSLNPWRLPLIAVFDNQQIGVITRRDNHDNISVQFSG
                     DEGLETTLNVADIEDKIVELALLRPLSAIPDARVDDYIRPYQANWFWSLSLKDWRRYG
                     DIMLASLVANVLALAAMIFSMQVYDRVVPAQSYPTLWVLFAGVMMAILFEFCMRMVRT
                     HLSDVIGKRADLRISDRVFGHALRLKNNVRSKSTGSFISQIRELESVRELITSTTIGA
                     VADLPFFLLFVFILWMIGGWLVLVVLLALPLLVIPGLLVQRPLARLANEGMRESAVRN
                     ATLVEAVQSIEDIKLLRAEQRFQNQWNHTNDVASSISMKQRFLTGLLLTWTQEVQSIV
                     YVVVLLVGCFMVMNGDMTTGALVGTTILASRTIAPLSQISGVLSRWQQAKVARNGLDE
                     LMKRPVDQPEHGKLVHKAVLHGNYQFSNAVFYYDEEEKIADVAIGKLNIQAGEKIAIL
                     GRNGAGKSTLLQMLAGMRIAQQGQVLLDNISIGQLDPADLRRDMGLLSQTGRLFFGSL
                     RENLTMGMPEASDEDIERALTLSGALPFVQKQKNGLNYMIQEGGFGLSGGQRQTLLLA
                     RLLISQPNIVLLDEPSASLDEMAEAYLIEQLKQWIGHRTLIIATHRTAMLQLVDRIIV
                     MDQGRIVMDGAKEAILREQGEPTARRVVLQEKNKGSAA"
     gene            complement(14962..30699)
                     /locus_tag="ACHJWA_000965"
     CDS             complement(14962..30699)
                     /locus_tag="ACHJWA_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFO4307612.1"
                     /translation="MDINTIATDDIINASEKGADLQISGTSNQPAGTTITLALNGQNY
                     TATTDAAGNWSTTVPASAVGALGEASYTVTANVTDSAGNSNSASHNVQVNTALPGVTI
                     NPVATDDIINAAESGNAQTISGQVTGAAAGDTVTVTLGGKTYTATVQGNLSWSVDVPA
                     ADIQAIGNGNLTVNASVTNGVGNTGSGSRDITIDANLPGLRVDTVAGDDVVNSIEHAQ
                     ALVITGSSSGLAAGAALTVVINTVTYAATVLADGTWSVGVPAADVSNWPAGTVNITVS
                     GTNTAGTTSTITHPVTVDLAAVAISINTVSGDDVINAAEKGADLTLSGSTSGVEVGQT
                     VTVTFGGKTYTATVAGDGSWTTTVPAADLSVLRDGDATVQASVSTINGNTASATHAYS
                     VDATAPTLAINTIATDDILNAAEAGNPLTISGSSTAEAGQTVTVTLNGVTYSGSVQAD
                     GSWSVSLPTADLSNLTASQYTVSASVSDKAGNPASANHGLAVDLTVPVLTINTVSGDD
                     IINAAEHGQALVISGSSTGGEAGDVITVTLNSKTYTTMLDASGNWSVGVPAADVTALG
                     SGPQTITAAITDAAGNSDDASRTVTVNLAAPTIGINTIATDDVIKATEKGADLQITGT
                     SNQPAGTTITVTLNGQNYTATTDSNGNWSATVPASAVSALGEANYTVTANVTDTAGNS
                     NSASHNVLVNSALPAVTINAVATDDIINAAESGNAQTISGQVTGAAQGDTVTVTLGGN
                     TYTATVQSNLSWSVDVPAADIQALGNGDLTVNASVTNGVGNTGSGSRDITIDANLPGL
                     RVDTVAGDDVINSIEHNQALVITGSSSGLTAGTALTVEINNVTYGATVLADGTWSLGV
                     PAVDVSNWPAGTVNITVSGTNSAGTTSTITHPVTVDLAGVAITINTLSGDDVINAVEK
                     GETLVVSGSTSGVEAGQTVTVTFGGKNYTTTVEANGSWTVNVPPADLAALPDGAGNVQ
                     ASVSNINGNSAQADRAYSVDATAPLVTINTIASDDILNVSEAGAGITISGTTTAQAGQ
                     TLTVTLNNNTYQTTVLADGTWSVNVPAADLSGLTASSYTVTATVSDKAGNPASADHAL
                     VVDITAPDLTINTVAGDDIINAIEHGQALVVSGTSTGAAAGDVVTVTLNGKNYTTTLD
                     ASGNWSVGIPAADVTALATGSQTITASLSDRAGNSDSTTHDVTVDLSGPTLTINTVSG
                     DDIINAAEIVVAQTISGQVTGTAVAGNTVIVTIGGNQYNATVQSDLSWSVSVPANVLQ
                     ALGNGELTISASLTNSANNTGTATHDIVIDANLPGLRVDTVAGDDVINSIEHTQALVI
                     TGSSSGLAAGAALTVVINSVTYGATVLADGSWSVGVPVADVTNWPAGTVNIAVSGTNT
                     AGTTTSISHPVTVDLAAVAITINTLSTDDVINAAEKGSDLQLSGTTSGVEAGQTITVI
                     FGGKSYTTTVAADNTWGLTIPAVDVATLPDGAANVQASVSNVAGNSTQATHAYSVDAT
                     APSVTINTIATDDILNAAEAGSALTISGTSTAEAGQTVTVTLNGVNYSGNVQADGSWS
                     VSVPTGDLASLTASSYTVNASVSDKARNSASATHNLTVDLAAPVVTINTVAGDDIINA
                     TEHGQAQIISGSATGATTGNTVSVTIGTTTYTTVLDANGNWSIGVPASVISALAQGDV
                     TITATVTDSAGNSGTASHTVTVALGAPVLAINTIAVDDIINAAEKGADLAITGTSNQP
                     AGTQITVTLNGQNYTTTADASGNWSVTVPASRVSALGEATYTVTAAATDADGNSGSAS
                     HNVQVNTALPGVTINVVATDDIINAAEAGVEQTISGQVTGAAAGDTVTVTLGGATYTA
                     TVQANLSWSVDVPASALQELGNGELTISASVTNSVGNTGNGTREITIDANLPGLRVDT
                     VAGDDVVNIIEHGQALVITGSSSGLAAGSNVTLTINGQTYVAAVLADGTWSVGVPAVD
                     VSAWPAGSVTIAASGSTSAGNPVSVTHPVTVDLSAVAVSINAITADDVINAAEKGAAL
                     TLSGSTSGVEAGQTVTVTFGGKTYSATVAANGSWSTSVPAADMAALRDGDASAQASVS
                     NVNGNSATTTHAYSVDASAPTVTINTIAGDDILNAAEAGAALTITGSSTAEAGQTVTV
                     TLNGTNYTGTVQTDGSWSVSVPSADLSTLTASNYTVNAAVSDKAGNPASVNHNLTVDT
                     SVPVVTINTVAGDDVINATEHAQAQIISGSATGAATGSTVTVTIGTNTFTTVLDASGN
                     WSVGVPASVVSALANGTVTINASVTDAGGNSGSATHQVTVNTGLPTITFNAISGDNIL
                     NADEKGQPLTISGGSTGLATGAQVTVTLNGHNYSATTDASGNWTLTVPVSDLAALGQA
                     NYTVSASATSAAGNTASSQANLLVDSGLPDVTINTVAGDDIINAAEAGADQTISGVVT
                     RAAAGDTVTVTLGGNTYTATVQSNLSWSVSVPTADLQALGNGDLTITASVTNANGNTG
                     SGTRDITIDANLPGLRVDTVAGDDIVNSIEHGQALVITGGSSGLNAGAVLTVTINSVA
                     YSATVQADGSWSVGIPAANVSAWPAGPLTVEVDGQSSANNPVSVSHPFTVDLTAVAIS
                     INTVASDDVINAAEKGTNLTLSGSTSGIESGQTVTVTFGGKTYTASVAANGSWSVNVP
                     AADLATLPEGAANVQASVSSASGNSASATHAYSVDASAPTLTINTIASDDILNAAEAG
                     SPLTISGTSTAETGQTVTVTLNGATYTGTVQADGSWSVSVPTSALGALNASNYTVSAT
                     VNDKAGNPGSASHNLAVDTTAPVLTINTVAGDDIINDAEHAQALVISGTSSGGEAGDV
                     VSVVLNGKTYTTTLDASGNWSVGVPAADVTALGSGAQTITASVSDRAGNSDDASRTVT
                     VSLSAPVISINTIAGDDVINATEKGSDLALSGTSDQPAGTAITVTLNGQNYSATTDAS
                     GNWSVTVPASAVSALGEATYSVTASVTNAQGNSSTASHNVQVNTALPGITINPVATDD
                     IINASEAGSAQTISGQVTGAAAGSTVTVELGGKTYTATVQADLSWNVSVPAADWQALG
                     NGELTVNASVTNAVGNTGSGTRDITIDASLPGLRVDTVAGDDVVNIIEHAQAQVITGS
                     SSGFAAGTALTVVINNQTYAATVLANGSWSVGVPATDVSNWPAGTLNITVSGANSAGT
                     QTSITHPLTVDLTAVAISMNSITSDDAINAAEKGAALTLSGSTSGVEAGQTVTVTFGG
                     KTYTTTVAANGSWSTTVPAADLAALRDGDASAQVRVTNVNGNSATATHEYSVDSAAPT
                     VTINTIASDNIINASEAAAGVTVSGTSTAQTGQTLTVTLNGTNYQTTVQTDGSWSLTL
                     PASDLTALANNGYTLTATVSDLAGNLGSASKGVTVDTTAPVISFNTVAGDDVINNVEH
                     IQAQIISGTATGAVAGDRLVVTIAGQQYVTSTDASGNWSVGVPASVISGLADGTVTIS
                     ATITDSAGNSSTQTHNVQVNTAAVSLSVSTISGDNLINAAEAGSALTLSGTGTNFATG
                     TVVTVLLNGKGYSATIQSNGSWSVNVPAADVAALSDGTSYTVSASAQDSAGNSATASR
                     SVAVDLTAPVISINTVSTDDRLNAAEQQQPLTLNGSTSAEVGQTVTVTFGGKTYTATV
                     AANGTWALNVPAVDLAALGQGAQTITASVNDRAGNPGQATHALTVDTVAPTVTIATVA
                     GDDIINNAEQLAGQTISGTTTAEVGQTVTVTFNGQTWSATVGSGGSWSVFIPAQQFAG
                     LSDGSYTISATVSDQAGNPGSASRGVTLNGDVPTVTINTFAGDDVVNAAEHGSSLVIS
                     GTTTAPVGQTLTLTLNGKTYTTTVQTGGSWSYTLGSADVTALADGNAYVINASVSNAI
                     GNTGSSNHTITVDLSAPAMGINIDSLQADTGLSASDFITSVSPVVVNGSLTAALASNE
                     TAQISIDGGTTWTTLTVTGTTWRYNDSRTLTDGNYLYQVRVIDAAGNVGATDSQNVVI
                     DTTAPDPAVKTIAISAITTDMGLITNDFVTSDTTLAVSGTLGATLSAGEFAQISLDGG
                     VTWTTLTVVGTSWSYADGHTLTDGTWNYTVRVVDLAGNVGQTATQNVVVDTTSPEAAK
                     SITITGISDDTGTSSSDFITSDTTLTVRGVLGAALGANEFAQISTDNGATWVNVTVAA
                     DSLNWSYVDGRTLTNGTTTWQVRVVDLAGNVGATSSQSALIDTVNPAQVLTIASISTD
                     TGSSATDFITSDTMLTLTGSLGAGLASGEVAQISLDSGATWTTLTTNGTQWTYTDSRT
                     LTDGSYVYQVRVLDLAGNTGPVVSKTVVVDTINPTATPTIVSYTDDVGQRQGTLSSSQ
                     ATDDTTPLLNGVLSAPLASGEVVYLYRNGLLLGAVTMVGALNWTYSDSGLVSGAYTYS
                     ARVVDLAGNITSSSDFVLTVDTSIPTTLAQITSQTTRDTTPIISGVITAALASGQYVE
                     VVINGKTYTSEPGGAVVVDPAHNTWYVQLPDTDALTVSATAYTVTAQVKSSAGNGNNA
                     NISNGTVTVNAAIDYTPTWTTASKTTAWGLTYGLDSHGMWTVLANQQVMQSTDPLTWS
                     KTALTLYQSGNNYATSSIADYDRNGTGDLFITRDDYGTGYINGFTNNGDGTFSSAIQV
                     TVGTLTWYGSIVAFDKEGDGYLDFWIGDAGGPDSNTFLWNNAGTLVGNSTTSNSGGSA
                     TVGGAVTGYLSLNEGSGVDLNNDGRIDLVQHTYNLNNYYTLSSLINQGNGTFVWGQNT
                     TNTFLSGAGSGAMSSSVSMTWADFDGDGDMDLFLPASQGRANYGSLLFNTNGVLGSPV
                     AVGATATTYASQFSLAVDWNHDGLMDIARIAQTGQSYLYTNVSNASNWTQSALGGSQS
                     GTTSGVAAMDYDWDGAVDVLVSKQSGSVFLSRNTNTVSYGTSLHLRITDPNGINVYYG
                     NTVKLYNSAGVLVATQIINPQSGMGVNDTSALVNFYGLNAGETYNAVLIKSTGTTASN
                     IDQTVNTSWGGLQATDATHAYDLSAEAGTASNNGKFVGTGYNDTFFATAGTDTYDGSG
                     GWVYSSGTGTWLANGGMDVVDFRLSTVGVTANLSSTAAQATGFNTSTFTNIEGISGSN
                     FNDILTGSSGDNQLEGRGGNDTLNIGNGGHDTLLYKLLNASDATGGNGSDVVNGFTVG
                     TWEGTADTDRIDIRELLQGSGYTGNGKASYVNGVATLDAQAGNIGDFVKVTQSGSDTI
                     VQIDRDGTGGTFATTNVVTLTGVHTDLATLLANHQLMVV"
     gene            complement(30736..35121)
                     /locus_tag="ACHJWA_000966"
     CDS             complement(30736..35121)
                     /locus_tag="ACHJWA_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFO4307613.1"
                     /translation="MSLIIDVISRKTSVKQTLINPGDVTVVIYEPSVVQVHAQASAVA
                     RYVREGNDLLIYMQDGTVIRCNGYFLQAANTAEQSELVFADGQQLTHITFADTAAGGL
                     APVELTAQTTAIESIAPFLDTVAQTSAFPWGWLAGAAVGGGALGALLASGGDGDSKTE
                     VINNPTPPAEPGNATPSFLVTDNQGDQRGILATNDITDDTTPTFSGSGQAGATIQIKD
                     SNGNTIASTQVDNNGHWSVSLPTQSAGEHTWSVVQIVGSTITDAGSITLTIDNSQASV
                     QVATTAGDNIINASEQAAGFTLSGTSSHLAQGTELTVTLNGKTYTTSVGANGAWSVQV
                     PTADAQALGEGNQAVLVSGKDATGNTVTGAQLLTVDTQPPTLAINTIAQDNIISAAEH
                     NVALVLSGTSNAEAGQTVTLTVNGKSHTATVGSDGTWQVTLPATEVQALAEGNYAVNA
                     SVSDRAGNTTSHSANFTVDTSAPVVSVNTVAGDDILNNAEQAVAQIISGQVSGASPGD
                     TVTVKLGTHVLTGIVLADGSWNVALDPAVTRTLDRGANTIFVTVTDAAGNTGAASRAI
                     TLVGVSPLITINTVSGDDIISGAEKGAPLTLTGSTQQAETGQTVTVTLAGQSFTTTVQ
                     ADGSWSLTVPAAAMGNLPDGAVAITASVTDLSGNTGNTSRTITVDSQAPALSIDPLTA
                     DNIINAAESGQDLPITGTTDAQPGQTVTVTLNGQTYQGVVQPDGTWSVTVPAANVGAL
                     ADGNATVTASVNDVAGNPSSVSRVALVDATPPVVTINPVATDNVINTPEHAQAQIISG
                     TVTGAQAGDIVTVTLNNVDYTTVVDGSGNWSLGVPASVVSGLADGSYPVSVSVTDKAG
                     NTGSQSLTVTVNTAAPLIGINSIAGDDVINASEKGADLQITGTSDQPVNTAITVTLNG
                     QNYTTTTDASGNWSVTVPASAVTALGQANYTVTAAVTSDIGNSATASHNVLVDSALPG
                     VTINPVATDDIINAAEAGVAQTISGQVTGAEDGDTVTITLGGNTYTATVGSNLTWSVD
                     VPAADIQALGNGDLTVNASVTNQNGNTGSGTRDITIDANLPGLRVDTVAGDDVVNIIE
                     HGQALVVTGSSSGLAESTPLTVTINNVEYTTAVQADGSWSVGVTAAQVSAWPAGTVNI
                     AVSGESSAGNSVSITHPVTVDLTPAAITINTIATDDVINAAEKGADLTLSGTTTNVEP
                     GQTVTVTFGGKNYTASVASDGSWTATVPAADLASLPEGSASALASVSNINGNSASAVH
                     NYSVDSSAPTIIINTVASDNIVNASEADAGVTVSGSTTAEAGQIVTITLNSPTVQTYQ
                     ATVQADGSWSINIPAADLEALTDGSHTLTATVNDKAGNPASTTHNLAVDLTVPVLTIN
                     TIAGDDIINATEHGQALVISGSSTGGEAGDVVTVTLNSKTYTTTLDASGNWSVGVPAA
                     DVTALGSGPQTVTATVTDAAGNSDN"
     gene            complement(35224..36579)
                     /locus_tag="ACHJWA_000967"
     CDS             complement(35224..36579)
                     /locus_tag="ACHJWA_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HFO4307614.1"
                     /translation="MARFQFKNRKNNGLIFFISFMVMGEAAIAAPLPQWANAPAVTPV
                     AQLSLQESILRAFARNPGVTQQAAQIGIGEAQIDEAKSAWYPHVGLTGNAGPSRQTDS
                     SGRLDNNVSYGITLTQLVYDFGKTNNDINLQTAARDSYRFKLMATLTDVAEKTATAYM
                     EVSRYQALCDAAQRNIHSLENVYNMAALRANAGLNSSSDELQAQTRIAGMRSTLEQYQ
                     AQMASAKAQLAVLTGVQPEAIAAPPAELAEQPVSLKNIDYQSIPLVLAAENLRQSAQY
                     GVEKTKAQYWPTLSIQGGKTRYQTSDRSYWDDQLQLNVNAPLYQGGAVSAQVQQAEGQ
                     QKISASQVEQAKLDVLQRASVAYANWTGARGREEAGLAQSESAHKTRDVYQNEYKLGK
                     RSLNDLLTVEQDVFQAQSAEINANYDGWVAAVNYAAAVNNLIPLAGIKQGLYNDLPDL
                     K"
     gene            complement(36917..38209)
                     /gene="ybaT"
                     /locus_tag="ACHJWA_000968"
     CDS             complement(36917..38209)
                     /gene="ybaT"
                     /locus_tag="ACHJWA_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFO4307615.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            complement(38212..39144)
                     /gene="glsA"
                     /locus_tag="ACHJWA_000969"
     CDS             complement(38212..39144)
                     /gene="glsA"
                     /locus_tag="ACHJWA_000969"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFO4307616.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTSDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGELVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            39406..41910
                     /gene="copA"
                     /locus_tag="ACHJWA_000970"
     CDS             39406..41910
                     /gene="copA"
                     /locus_tag="ACHJWA_000970"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFO4307617.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADFDEAQALRLAAALEQGSSHPLAR
                     AILDKASDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            complement(42125..42466)
                     /gene="ybaQ"
                     /locus_tag="ACHJWA_000971"
     CDS             complement(42125..42466)
                     /gene="ybaQ"
                     /locus_tag="ACHJWA_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO4307618.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            42604..43398
                     /gene="ybaP"
                     /locus_tag="ACHJWA_000972"
     CDS             42604..43398
                     /gene="ybaP"
                     /locus_tag="ACHJWA_000972"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFO4307619.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDISLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQKLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            43602..44081
                     /gene="ybaK"
                     /locus_tag="ACHJWA_000973"
     CDS             43602..44081
                     /gene="ybaK"
                     /locus_tag="ACHJWA_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFO4307620.1"
                     /translation="MTPAVKSLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(44118..45770)
                     /gene="ushA"
                     /locus_tag="ACHJWA_000974"
     CDS             complement(44118..45770)
                     /gene="ushA"
                     /locus_tag="ACHJWA_000974"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFO4307621.1"
                     /translation="MKLLQRGVALALLTTFTLASEAALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKLGNPENFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGDHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            45988..47208
                     /gene="fsr"
                     /locus_tag="ACHJWA_000975"
     CDS             45988..47208
                     /gene="fsr"
                     /locus_tag="ACHJWA_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFO4307622.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            47445..49121
                     /gene="ybaL"
                     /locus_tag="ACHJWA_000976"
     CDS             47445..49121
                     /gene="ybaL"
                     /locus_tag="ACHJWA_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFO4307623.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(49254..50558)
                     /gene="gsk"
                     /locus_tag="ACHJWA_000977"
     CDS             complement(49254..50558)
                     /gene="gsk"
                     /locus_tag="ACHJWA_000977"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFO4307624.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            50710..51669
                     /gene="aes"
                     /locus_tag="ACHJWA_000978"
     CDS             50710..51669
                     /gene="aes"
                     /locus_tag="ACHJWA_000978"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFO4307625.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPPWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATKAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQVIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKVAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            complement(51666..52628)
                     /gene="hemH"
                     /locus_tag="ACHJWA_000979"
     CDS             complement(51666..52628)
                     /gene="hemH"
                     /locus_tag="ACHJWA_000979"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFO4307626.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(52760..53404)
                     /gene="adk"
                     /locus_tag="ACHJWA_000980"
     CDS             complement(52760..53404)
                     /gene="adk"
                     /locus_tag="ACHJWA_000980"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFO4307627.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            complement(53585..55459)
                     /gene="htpG"
                     /locus_tag="ACHJWA_000981"
     CDS             complement(53585..55459)
                     /gene="htpG"
                     /locus_tag="ACHJWA_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFO4307628.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            complement(55569..56174)
                     /gene="recR"
                     /locus_tag="ACHJWA_000982"
     CDS             complement(55569..56174)
                     /gene="recR"
                     /locus_tag="ACHJWA_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFO4307629.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            complement(56174..56503)
                     /gene="ybaB"
                     /locus_tag="ACHJWA_000983"
     CDS             complement(56174..56503)
                     /gene="ybaB"
                     /locus_tag="ACHJWA_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFO4307630.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            complement(56556..58487)
                     /gene="dnaX"
                     /locus_tag="ACHJWA_000984"
     CDS             complement(56556..58487)
                     /gene="dnaX"
                     /locus_tag="ACHJWA_000984"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFO4307631.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDAWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEDSIRPI"
     misc_feature    complement(57159..57223)
                     /gene="dnaX"
                     /locus_tag="ACHJWA_000984"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            complement(58616..59167)
                     /gene="apt"
                     /locus_tag="ACHJWA_000985"
     CDS             complement(58616..59167)
                     /gene="apt"
                     /locus_tag="ACHJWA_000985"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFO4307632.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGINKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            complement(59320..59697)
                     /gene="ybaN"
                     /locus_tag="ACHJWA_000986"
     CDS             complement(59320..59697)
                     /gene="ybaN"
                     /locus_tag="ACHJWA_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFO4307633.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            59767..60294
                     /gene="priC"
                     /locus_tag="ACHJWA_000987"
     CDS             59767..60294
                     /gene="priC"
                     /locus_tag="ACHJWA_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFO4307634.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFNRHLFQTRAT
                     TLQACLDKAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREATAWSLREWDSAP
                     PQIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            60308..60472
                     /gene="rsmS"
                     /locus_tag="ACHJWA_000988"
     CDS             60308..60472
                     /gene="rsmS"
                     /locus_tag="ACHJWA_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFO4307635.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LQSDEASQSE"
     gene            complement(60511..63873)
                     /gene="mscK"
                     /locus_tag="ACHJWA_000989"
     CDS             complement(60511..63873)
                     /gene="mscK"
                     /locus_tag="ACHJWA_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFO4307636.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRVKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(64001..64648)
                     /gene="acrR"
                     /locus_tag="ACHJWA_000990"
     CDS             complement(64001..64648)
                     /gene="acrR"
                     /locus_tag="ACHJWA_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFO4307637.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            64790..65983
                     /gene="acrA"
                     /locus_tag="ACHJWA_000991"
     CDS             64790..65983
                     /gene="acrA"
                     /locus_tag="ACHJWA_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFO4307638.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            66006..69155
                     /gene="acrB"
                     /locus_tag="ACHJWA_000992"
     CDS             66006..69155
                     /gene="acrB"
                     /locus_tag="ACHJWA_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFO4307639.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSIEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHNHTVDHH"
BASE COUNT        14901 a        19149 c        18889 g        16333 t
ORIGIN      
        1 tatatttaaa attattaaac tccaccgatt cagattaata tcagcaatag aaagatagga
       61 aatcaccaat tcaataacat catcaatgaa acatattgta gaattaattt aaacaaggcg
      121 ttccattcaa ccagaacgcc ccttatttac tctcatcacc ccgcaaactg cctgaacagc
      181 gccttaccct taaccagtct cgcgcccagc cagccgccga aaagcgacag cagcagcgca
      241 ccgctgcacg gcagaacaat ccacaatcgc cagtctggct cccacggaaa atcaaacact
      301 ttcgcctgca acactgccag tgccgtttcc gcaccaattg cggccaccag gccggaaaca
      361 aacccaagca tggcgaactc acaccacaac gtggtacgca gcagtttttt ccccgcaccg
      421 agtgtgcgcc acaccaccag ctcctgatga cgctgacgca ttcccacctg cacctgtgcc
      481 agcaacagca acataccgca ggcggtgacc agcaccacca tcacctccag cgcccgactt
      541 acctgctcca gcacctgacc gacctgtttt aaaatcgcgc caatgtctaa caggctaatg
      601 gtagggaact ggcggttgag ttgcgtcaac atgccgttgc cattctccca gcggaaactg
      661 gtaagccagc tctgcggttg cccgtctaat gccccttcag ggaaaataaa atagaaatta
      721 ggccgcagac tttcccagtc cactttgcgc aggctggtca ctttagcgcg gaactcctgg
      781 gtatcgccca taaaagtcac ggtatcgccg agggtaacgt ttaagcgttt cgccagcccc
      841 tcttccatcg aaacttcatc agcttttggc ggccagttac cggcgacaat cggattatga
      901 tcgggccgcg tattttgcca ggtaagattg agttcgcggt tgagcgcctc atcttcattg
      961 ccttctgtcg gcttgccgtt aatcgccgtc agccgcgctc gcaccaccgg ataaaacgat
     1021 tccgggacta tctgatgttc cgcgaggaac gcttttagcg gcgtaacctg ttctgtggcg
     1081 atgttgatta aaaagtagtt cgggctttct ggcggtaact gctgctgcca gcggtcgagc
     1141 agatcgccac gcagcaccag tagcagtgcc agcagcataa aggagagcga aaataccgaa
     1201 agctggctta acgttgacca cggctgacgt aacaggcggc taaccgccag gcgcagaggc
     1261 agcgatttca gcgtcatgcg gcgaagtaca ttcagcagca tccagcccag cacaccgcac
     1321 agcaaagcca gtactaccgc gcccgccagc accgcccaaa gcagcatgct gccacccatt
     1381 aatccggcga gcagcagcac aaccaccaca ctgacaatcg gcagataaaa cttcagcggc
     1441 cagacgttcg caaccacatc attacgtaat acgcgtaaag gctgcgttgc cagcaacaag
     1501 cggtatggtc gtagccccac cagcagcgag atgaccgtca tggtgcccag cgcccatagc
     1561 catggccaga ggctggctgg cggtagtgca gcaggcagaa cgggcttgag cagcaccatc
     1621 aacacgtttt cgaacaacag gcctatcgcc ccaccggtaa cggctgaaag cgtcagcacc
     1681 atcaaccact gaccgacgat tagcttacgc agttgcgctc gccctgcccc cagcgttttg
     1741 aggatcgcca ccagatcgta gcgactgcga cagtaatgat tcatcgctac cgccactgct
     1801 gccactgcca gcagcaaggt cagaagtgcc gaaagcagca ggaactgttg cgagcgttcc
     1861 atcgatcgcc ccagcgcgcc ttcgtcctgt tccagaccgt accagcgttg ttcgggttta
     1921 agctgaggta acaaccattt ctcatagctg tcgagctggt tctcgctgcc gccgaattta
     1981 tagcgccagg tgacccgact ccccggctgc acggctccgg ttttatcgac atccgccaga
     2041 ttcatcatca gacgcggagc catctggaag gggttaaaac cggaatccgg ttcctgaatc
     2101 acttctccgg caatccgcaa ggtggcatcg cccacgtcaa tggtgtcgcc cgttttcagg
     2161 ttaagcagtg ccatcaggcg tggggccagc aatacgctgc ccgcctgcgg tttcaggcca
     2221 gggggattgg tttgcagatc gccatacatc gggtagatat catccaccgc tttaacgttc
     2281 gccagctgcg gtgtgtcgcc tgcaaatgtc atggtggcga aagtcagctg cttgccgact
     2341 ttcaggccgc gcttttgcgc ttcctccagc cacgcttgcg gcacttcgcg cgaactgcgc
     2401 aacgcccgat cgcccgccat aaactcccgg ctttgttggc ttaagccctt ctccatgcga
     2461 tcgctgatat tgcccagcgc cagcacgcag gccaccgcca ggcttagcgc cagccagaca
     2521 attaatagcg acggcgagcg ccattcgcgc cagaaccagc gtgcaatcat gcttcctcct
     2581 gcaactgccc gttcaccagc cgtaagcagc ggtcgcagcg tgccgccagt tgcaggtcgt
     2641 gggtcaccat aatcaacgtg gtgccatgct cacggttgag ggaaaacagc aggtcggcaa
     2701 ttttatcgcc cgtctggcgg tcaaggttac cggtgggttc gtcggcaaac agcacatcag
     2761 gtcgaccgtt aaaggctcgt gccagcgcca ctcgttgctg ttcaccgccg gaaagctgtg
     2821 ccggaagatg atccagacgt ttacccagcc ctaactgttc gagcaacgct ttcgctccgt
     2881 tacgactttc cgcgctactc tcaccgcgca gcagcgccgg aagctcgacg ttttccagcg
     2941 cgttaagggt aggaattaac ataaatgact gaaaaacaaa gccgacgtgc ttcgcgcgca
     3001 actttgcccg cgcttcttcg tccatattat gtagcggttg tcccaccaga ctcacttcgc
     3061 cactgctgcc gtcatccagc ccggcgagga tcgccagcaa ggttgactta cccgatcccg
     3121 actcgcccac cagtgcgatg gtctcgccac gtttgacaac cagctcaact ccggtgagga
     3181 tggagagttc atgctccccc tgaccgacgg acttcttaag atgatgaact tcaacaatgt
     3241 tttccgctgg catttgccct tcctgtttct ggtcctgtta accttccgtg ctgccgcagc
     3301 ggacacgtta ttgattctgg gtgatagcct gagcgccggg tatcgaatgt ctgccagcgc
     3361 ggcctggcct gccttgttga atgataagtg gcagagtaaa acgtcggtag tcaatgccag
     3421 catcagcggc gacacctcgc aacaagggct ggcgcgcctt ccggctctgc tgaaacagca
     3481 tcagccgcgt tgggtgctgg ttgaactggg cggcaatgac ggtttgcgtg gttttcagcc
     3541 acagcaaacc gagcaaacgc tgcgccagat tttgcaggat gtcaaagccg ccaacgctga
     3601 accattgtta atgcaaatac gtctgcctgc aaactatggt cgccgttata atgaagcctt
     3661 tagcgccatt taccccaaac tcgccaaaga gtttgatgtt ccgctgctgc cctttttaat
     3721 ggaagaggtc tacctcaagc cacaatggat gcaggatgac ggtattcatc ccaaccgcga
     3781 cgcccagccg tttattgccg actggatggc gaagcagttg cagcctttag taaatcatga
     3841 ctcataaagc aacggagatc ctgacaggta aagttatgca aaaatcggtc ttaattaccg
     3901 gatgttccag tggaattggc ctggaaagcg cgctcgaatt aaaacgccag ggttttcatg
     3961 tgctggcagg ttgccggaaa ccggatgatg ttgagcgcat gaacagcatg ggatttaccg
     4021 gcgtgttgat cgatctggat tcaccagaaa gtgttgatcg cgcagccgac gaggtgatcg
     4081 ccctgaccga taattgtctg tatgggatct ttaacaatgc cggattcggc atgtatggcc
     4141 ccctttccac catcagccgt gcgcagatgg aacagcagtt ttccgccaac tttttcggcg
     4201 cacaccagct caccatgcgc ctgttacccg cgatgttgcc gcacggtgaa gggcgtattg
     4261 tgatgacctc atcggtgatg ggattaatct ccacaccggg tcgtggcgct tacgcggcca
     4321 gtaaatatgc gctggaggcg tggtcagacg cgctgcgcat ggagctgcgc cgcagcggaa
     4381 ttaaagtcag cctgatcgaa cccggtccca ttcgtactcg cttcaccgac aacgtcaacc
     4441 agacgcaaag tgataaacca gtcgaaaatc ccggcatcgc cgcccgcttt acgttgggac
     4501 cggaagcggt ggtggacaaa gtacgccatg cttttattag cgagaagccg aagatgcgct
     4561 atccggtaac gctggtgacc tgggcggtaa tggtgcttaa gcgcctgctg ccggggcgcg
     4621 tgatggacaa aatattgcag gggtgagttg aagcgcgcgc ttaagccccc atgtcaaaga
     4681 aaatatcgac aacagagagc gactccatgt ccgtagaaaa tattgtcaac attaacgaat
     4741 ctaacctgca acaggttctt gaacagtcga tgaccactcc ggtgctgttc tatttttggt
     4801 ctgaacgtag ccagcactgt ttgcagttaa ccccaattct ggaaagcctt gcggcgcagt
     4861 acaacgggca atttattctg gcgaagctgg actgcgacgc ggagcagatg attgccgccc
     4921 agtttggtct gcgcgcgatt ccgaccgtgt atctgttcca gaacgggcaa ccggtagatg
     4981 gcttccaggg gccgcaaccg gaagaggcga tccgcgccct gctggataaa gtgctgccgc
     5041 gcgaagaaga gctgaaagcg cagcaggcga tgcaactgat gcaggaaggc aattacaccg
     5101 atgccctgcc attgctgaaa gacgcctggc agttgtcgaa tcagaacggg gagatcggcc
     5161 tgctgctggc agaaacgctg attgcgctga accgttctga agatgcggaa gcggtgctga
     5221 aaaccattcc gttgcaggat caggacaccc gctaccaggg gctggtggcg caaatcgaac
     5281 tgctgaagca ggcggctgat acgccggaaa ttcaacagtt gcaacagcag gtggcggaga
     5341 atccagaaga tgccgcactg gcgacgcaac tggcgctgca actgcatcag gttgggcgca
     5401 atgaagaggc gctggagttg ctgttcgggc atctgcgtaa agatctcacc gccgcagacg
     5461 gtcagacgcg taaaacgttc caggagatcc tcgctgcgct gggtacgggt gatgcactgg
     5521 cgtcgaagta tcgccgccag ctgtatgcat tgttgtattg atattccgtt gatcggcaca
     5581 tgaaccacaa cgccggatgc gatgctgcgc atccggcatc gcatcatttc ttcttcaatt
     5641 gcgtcaccac caactggtgg cgcgaattat aaaacttacg ataggttaaa tagcaggcaa
     5701 ttatggtcga caagctggcg gttgagagca gcataaaggt caccataatc tgatatttaa
     5761 tcgccttcac cggatcaatc ccggcaaata tcagcccgga catcattcct ggtaaactca
     5821 ctaagccaac cgtttttgct gaatcgaccg tcggaattaa agccgcgcga atactgtcgc
     5881 gaatcaatat cgctgaagcc tgcttcggcg tcgcgccaag actcagtttc tcctgaatct
     5941 gttgctgttc gctaatgacc cgttgcccta aattgttgta acacaacccc accgctacca
     6001 tggcgttacc ggcaatcatc ccggcgatag ggatcacctg catcgggata aattcaatcg
     6061 accctgagag aatcagcacc gccagggtaa ttcccgcccc gaccgtaatg gcaataaacg
     6121 atgagataaa agctttagca atatatttac tgcgtttttg cgcgttccac gccgcattaa
     6181 agcagataaa taacaccatc aataatgtca ggctggcatc atccacgctg aaaatatact
     6241 tcagcacata gccaacaata atcagctgaa ttatcgcgcg cccgacgctc cagagaatat
     6301 ctttctccag cgccagtttt tctttatggc taattaagat tgccaccact accagcatta
     6361 atgccagtgc taatgattcg ttagtaatat tatgcgagtt catagcgtgc ttcctgcatt
     6421 tctccggcat gcggttgcag tgtaatcact ttatccgcat gattaatttc gtctttatcg
     6481 tgtgtcaccc acagcacggc aatattttgc tcgcgcacat aacgatggat catctcatta
     6541 acgttatgtt tattactttc atccagcgca ctggtgattt catccagcaa taaaaccttc
     6601 ggcataaatt gcaggttacg aatcaatgag atgcgttgtt tttcaccacc agatagctcg
     6661 gcgatattct tcgtcaaaat gctgtccggc aaggcgaagc gttcaagaaa atcgagaaaa
     6721 atggccgggt caggctgctg gttacggatc tgccagggaa agatcagatt atcgtatacc
     6781 gtatcgccaa acagcgtcgg cgtctgggcg cagtaagaga cttgctgacg gtagatttct
     6841 ggctttagtg tgctgacatc ctcaccttca aacagtaacg ttccgctggt tgggctgatc
     6901 aatgaagcaa ctatttttag cagcgtactt ttgccacaac cagaaggacc ggtaattaac
     6961 ttaaattcgc cagcacgcag cgaaaaattg atgttattaa gaatcttcgc atcacccgcc
     7021 agatatccta cgttttgtag ctgaagcaaa ggactatttt cctgcatcgc tgttcccttt
     7081 ttctgatttt tactaaaaac agtttatcct tcgcaggaat aagggggaac tctctttcag
     7141 taatcaggta taatttgttt aatcaagcag caattttgct gattatggac ggtacaacag
     7201 gaggtttttc cgatgcttat ctttatcccg attctcattt ttgtcgcgct ggtcattgtc
     7261 ggcgcgggcg tcaaaatcgt gccgcagggc tatcaatgga cggtagaacg ttttggtcgc
     7321 tataccaaaa cgttacagcc ggggctcagt ctggtggtgc cgtttatgga tcgcattggt
     7381 cgcaagatca atatgatgga gcaagtgctc gatatccctt cccaggaagt tatctcgaaa
     7441 gataacgcca acgttaccat cgacgcagtc tgttttattc aggtgattga cgcgccacgc
     7501 gcggcttatg aagtcagcaa tctggagctg gcgatcatca acctgaccat gactaacatc
     7561 cgtaccgtgt tgggttcaat ggaacttgac gaaatgctct ctcagcgcga cagcatcaac
     7621 tcacgcctgc tgcgtattgt cgatgaggcc accaacccgt gggggattaa agtcacccgt
     7681 attgaaattc gcgacgtgcg cccaccggca gagcttatct cttcaatgaa cgcgcagatg
     7741 aaagcggaac gtaccaaacg cgcttacatt cttgaagcgg aagggatccg tcaggcggaa
     7801 atcctcaaag ccgaaggtga aaaacagtcg caaatcctga aagcggaagg cgaacgtcag
     7861 tcggcgtttt tacaggctga agcgcgtgaa cgttccgctg aagcagaagc ccgcgccacc
     7921 aaaatggtgt ctgaagccat cgcctccggt gatattcagg cggtgaacta cttcgtagcg
     7981 cagaaataca ccgaagcgtt acagcagatc ggttcctcca gtaacagcaa agtagtgatg
     8041 atgccattag aggccagcag cctgatgggg tcgattgccg ggattgccga gctggtgaaa
     8101 gacagcgcca acaagcggac tcagccatga tggagttaat agtcgttcat ccacatattt
     8161 tctggctcag tctcggcggt ttgctgctgg cagccgagat gctgggcgga aatggttatt
     8221 tgttgtggag tggcgtggca gcagtgatta ctggcctggt ggtctggctg gtgccgctgg
     8281 gttgggagtg gcaaggggtg atgtttgccg tcctgacgct gctcgccgcc tggctgtggt
     8341 ggaaatggtt gtcgcggcgg gtgcgcgaac aaaagcacag cgacagtcat ttaaaccagc
     8401 gcgggcagca gctgattggc cgacgttttg tgctggaatc tccgctggtc aacgggcgcg
     8461 gtcatatgcg cgtcggtgac agttcatggc ctgtcagcgc cagcgaggat ctcggcgcag
     8521 gtacgcatgt tgaagtcatt gcgatagaag ggataacgct gatcatccgt gcggtcatcg
     8581 cctgatgcga cgctgacgcg tcttatcatg cccggaagtc tgcgcccgaa tcgtaggccg
     8641 gataaggcgt ttacgccgca tccggcagtc gtgcaccgac gcctgatgcg acgcgggcgc
     8701 gtcatatcac gccaaaaccg taggccgcct ccgccatgtt aaatgttaac tggcattggc
     8761 aatttactct tcccggcctt tactcatact tttttggtct tcatccggat agtgtttttt
     8821 tagatattcc aggacgtttt tattgacctt gtgttgcgta tacacccacc ctttccagta
     8881 atcaggctgg tccaggtaaa cttctggcgg aatggtgaaa tcagaaagcg ttaaccattc
     8941 ggctaacaga tcggggtttc gtttctgtat caactgcaac agcataatca gcgacatggc
     9001 agaggcagga gccgtactat cgccgcttaa atacttccac actgtcgacc ggttcgaacg
     9061 aaaaaggatc ggtagcaatg cccggtccag attcatttca ttaaaaatct tctcaaattc
     9121 attcatttaa attttcctgc ctggcgtaaa cctcttaaaa attgagattt atcaaagaaa
     9181 cgcattttag cacacatcag gaaccgcttc acgtttagtc cagaaacaga atttatttcg
     9241 cttatcaaaa caagtcttta ctctttttta cattgaaaga gcacgaaatg atttcctttt
     9301 ttatttatat aagaaaccat ttttgtttct tattgatggt gtttacgctt acaacagaca
     9361 aaaatgcgct ttacatcaca caaatggcgg cgtagatttc gattaaattg caacgcagtt
     9421 tatttcttaa aacaatatta tttgtttctt atagaaacat taatacgact tattttgaac
     9481 aagagaaaat gaatgaaaac tgtaaacgta gctttactgg cactcataat ttcagcaaca
     9541 tccagccctg ttgttttagc tggtgatacc attgaagcgg cggcaacaga gctttcagcc
     9601 attaactctg gcatgtcgca atcggagatt gagcagaaga ttacccgctt tttagaacgc
     9661 acagacaaca gccccgctgc gtatacctat ttgactgaac atcactacat cccttctgaa
     9721 acacctgata ccactcagac tcccactgtc cagacagatc ctgacgcagg acaaaaaacc
     9781 gttgccgcta caggtgatgt acagacaact gcccgttatc agagcatgat caacgcccga
     9841 cagtctgcgg taactgacgc ccagcaaacg caaattacag agcaacaggc gcagatcgta
     9901 gccacacaaa aaacgctcgc cgcgactgga gatacgcaaa ataccgcgca ttatcaggaa
     9961 atgattaatg ccagactggc ggctcaaaat gaggctaatc agcgcaccgc cactgaacaa
    10021 gggcagaaaa tgaatgcgct gacaaccgat gtggcagtac aacagcaaaa tgaaaggact
    10081 caatacgata aacaaatgca aagtctggcg caggagtctg cccaggcaca tgaacaaatt
    10141 gacagcctgt cacaagacgt aacccaaacg caccaacagt taaccaacac ccaaaaacgg
    10201 gttgcagata acagccagca aattaacacg ctcaataacc atttcagttc gctaaaaaac
    10261 gaagttgatg acaatcgtaa agaagccaat gcgggaactg catctgccat cgctatcgcc
    10321 tcacaaccac aggttaaaac cggtgacgtg atgatggtgt cagcgggagc gggaaccttc
    10381 aacggtgaat ctgcggtgtc tgtcggaaca tcatttaatg ccggaacgca tacggtactt
    10441 aaagccggta tttctgcgga tacacaatct gatttcggcg caggtgtcgg cgtgggatat
    10501 tcgttctaat atttcaatcc tcaatataaa taagagcaag gaagcttgcc gggttcatct
    10561 cttcattaat ttgtacatta tttaaggtta acaatgatga atagctccat taaatcgttt
    10621 tccctgctgg cggttatatt actggctggc tgtagttcac ccacttcccg catcgcagat
    10681 tgccaggcgc agggcgtcag tcatgacacc tgttacctcg cagaacagca gcgtcaggcg
    10741 gctattttaa gtgcatccga ggcacaggca tttaaaaatg cagaagccgc acaacacgcc
    10801 caggcggcaa agaaagccat ttataaagga tttggcatga cctttagaat gagcagtaaa
    10861 aactttgctt atctcaatga ttcattatgt gcaattgatg aagacaataa agatgccact
    10921 gtttatcagt caggtctata taacgtcatt gtttatcatc acacaggaaa agtcgcctta
    10981 atgaaagaag gccagtttgt gggttattta aaatgaagga gcaaaggaaa atacccctga
    11041 cgcatattat gattatcggt gcgtttattt ttgccttctt gcaagtagta ttattagcct
    11101 ccctggttca cgctgtgaat gttaacaacg aaatccagga aggcttattt cagtcggggc
    11161 gcattatggt agaaagtttg cagcatattc tttcggtgca aacggggatt cactgatttt
    11221 caccccgccc gatgatgaca gcagccggag agattttcga taatcgggca gtcggcgctg
    11281 tcatcaccgg ggcaggcatt cgccagcgcc agtagctggt tgcgcataga ttgcagttct
    11341 tcgatatgcc gttcaatctc cgccaccttc tccagcgtgc gacgtttgac gtcggcactg
    11401 tgacgctgcg ggtcgttaaa cagattcacc agctcgccgc tctcttccag gttaaagccc
    11461 acctggcgcg cctggcgcag taaggtcaat tcgttgagat gctgctgcgt gtaggttcga
    11521 taaccatttt cgctgcgcat cggcggcgtc accagcccct tctcttcata gaagcgaatg
    11581 gctttgctgg tcaggccggt aatttttgct acatcgctaa tgttcatcgt tcgcgcaacg
    11641 cctcctgtgc tttgttaaag ggtttcaaca gataatccag cacgcttttt ttgccggtgc
    11701 ggatatcgac cgtcgccacc ataccgggga atatcggaaa ctgctgtttg cttttgtttt
    11761 ccagatggtt gctgaaagtg cgaatgtaca cccgatagta ataaacatcg cgccgcactt
    11821 catcctgcac ggtgtctggt gatatcaccg ccacttctcc cggcaggccg ccgtagatgg
    11881 agtaatcgta ggcagttatt ttcaccagcg atttttgccc cgggtggata aacgccacat
    11941 cgcgcgggga gatttttgcc tcaatcagca gttgctcatc cagcggcacg atggtcatca
    12001 gtttaccacc gggggcgatg acgccgccaa ccgtggtgac atcaatatcc tgaacaatgc
    12061 ctcgcaccgg cgcggtaaag ttgagcctgg tgagagagtc ttcccgtcca cggatcaccg
    12121 aacgctgggt ttccacttcc gcattggctt ttgctaactc ttcgcgcgcc tgaacgtagt
    12181 attgcgtgcg cacgtcgctg agtttgtttt ccagttcatt ggcctggcgt tgcaggcgca
    12241 gtacttcgac gctgctcgct gccccctgtt ttaccagcgg ctgggtcatc gccagttcgc
    12301 gattgaccag gctaatcccc tgcgtcagcc cggtggtggc tttgttcaac ccgtcgcggc
    12361 gagtgttgta caacgccgtt tccgactcga taagttcggg gaatttattc agttcgtcag
    12421 ggaagaccag cggtttattg ctcacttccg cccgcagccg cgccgaggtt gccagcgccg
    12481 cgtacagccg cgacatcgct tcctggacgt cggattccgt tttggtgcgg ttaagctgcg
    12541 ccagcggctg gccctgttcg acaatatcgc caactttcac gtcgagatga tacagaattc
    12601 cgccttccag cgactgcacc acctgttcgc gcccggaagg ttctaccgtg ccgctgccgg
    12661 tggtgacttc atcaagctgg aagtaccacg cccagccgag aaaacagagc agcatcacag
    12721 cggtaacgcg taccgccagc gccgaacgcg gcaggcgggg ttcagtcaga ccacggttat
    12781 attgcagttg tccgttcatg ccgcagaccc cttattcttc tcctgcaaca ccacccgtcg
    12841 cgctgtgggc tcgccctgtt cacgcaaaat ggcctctttc gcgccgtcca tcacaatccg
    12901 cccctgatcc atcacgatta tcctatccac cagttgcagc atcgccgtgc ggtgagtggc
    12961 gataatcagc gtgcgatgcc caatccactg tttcagttgt tcgattaaat acgcttctgc
    13021 catttcgtcc agtgaggcgc tgggttcgtc gagcaggacg atattcggct gagagatgag
    13081 tagccgcgcc agcagcagcg tttgccgctg accaccagag aggccaaatc ccccttcctg
    13141 aatcatgtag ttgaggccgt ttttctgctt ctgcacaaag ggcagcgctc cgctgagggt
    13201 taacgcgcgt tcgatatctt catcgctggc ttccggcatc cccatggtga ggttttcccg
    13261 cagcgaacca aagaacagtc gcccggtctg gctgagtagc cccatgtctc gccgcagatc
    13321 tgccggatca agctgcccga tactgatgtt atccagcaac acctgcccct gctgggcgat
    13381 acgcattccg gcgagcattt gcagtagcgt cgatttaccc gcgccgttgc gcccgagaat
    13441 ggcgattttc tcaccagcct gaatgttgag tttgccaatc gctacatcgg caatcttctc
    13501 ttcttcgtcg taataaaaca ccgcgttgct gaactgataa ttaccgtgca gcaccgcttt
    13561 atgcaccagt ttgccgtgtt ccggctgatc caccgggcgc ttcatcagtt catcaaggcc
    13621 gttacgtgct actttcgcct gctgccagcg cgagagcacg ccggatatct gcgacagcgg
    13681 cgcaatggtg cgcgaagcca ggatcgtcgt cccgaccagc gcgccggtgg tcatgtcgcc
    13741 gttcatcacc ataaagcagc ccaccagcag caccaccacg tagacgatgg actgcacttc
    13801 ctgggtccag gtgagcagca gcccggtgag aaaacgttgc ttcatgctga tggacgaggc
    13861 cacgtcgttg gtgtggttcc actgattctg aaaacgctgt tcggcgcgca gcagtttaat
    13921 atcttcaatc gactgcacgg cttccaccag cgtagcgttg cgtaccgctg actcgcgcat
    13981 tccttcgttc gccagccgcg ccagcgggcg ttgcaccagc aggccgggga tcaccagcaa
    14041 cggcagcgcc agcaaaacca ccagcaccag ccagccgccg atcatccaca aaataaagac
    14101 aaacagcagg aagaatggca ggtccgccac cgcgccgatg gtggtggacg taataagctc
    14161 ccgcactgat tccagttcgc ggatctgcga gataaacgat ccggtggatt tcgatcgcac
    14221 gttgtttttc agccgtagcg catgaccaaa aacgcgatcc gaaatacgca ggtctgcccg
    14281 ctttccgatc acatcagata gatgcgtacg caccatgcgc atacagaact caaacaggat
    14341 cgccatcatc accccggcga aaagcaccca tagcgtcggg tacgattgcg ccggaaccac
    14401 ccgatcgtaa acctgcatcg agaaaatcat cgccgccagc gccagcacgt tcgccaccag
    14461 tgacgccagc ataatatcgc cgtagcgccg ccagtctttt agcgaaagac tccagaacca
    14521 gttcgcctga tacgggcgaa tatagtcatc cacccgcgcg tcagggatgg cgctgagagg
    14581 gcgcagcagc gccagttcga cgattttatc ttcaatatcc gcgacgttaa gtgtagtttc
    14641 cagcccttcg tcaccgctga actgaacgct gatattgtca tgattgtcgc gacgggtgat
    14701 cacgccaatc tgctgattat caaacaccgc gatcagcggc agacgccagg ggttaagcga
    14761 atcggcggaa aactccacca accgcaaccc catccctaac tgccgcgcca tgtcggtgag
    14821 taactcctcc cgcttactgt cgcgctccca gttgacggtc gcgcgcacat tctcctgaga
    14881 aaaatcgagc cgataatagc gcgccacggc gatcatcgcc tccagccagg cttccacgga
    14941 ctgggcattt tttttcatta tctacaccac catcaactga tgattcgcca gcaaggtggc
    15001 gaggtcggtg tgcacgcccg tcagcgtgac cacgttagtt gtcgcaaaag tgccgcccgt
    15061 gccgtcgcgg tcgatctgca cgatggtgtc gctgccgctc tgggtgactt tgacaaagtc
    15121 accgatgttt ccggcctgcg catccagcgt tgccacgccg ttgacgtagc tggctttgcc
    15181 gttgccggtg tagccgctgc cctgtaacag ttcacgaata tcaatgcgat cggtgtcggc
    15241 ggtgccttcc catgtcccta ccgtaaagcc attcaccacg tctgagccgt tgccgccagt
    15301 ggcgtcgctg gcgttgagca gtttatagag caaggtgtcg tggccgccgt tgccgatgtt
    15361 gagcgtgtcg ttgccgccgc gcccttccag ttggttatcg ccgctgctgc cggtcagaat
    15421 gtcgttaaaa ttcgagccgg aaatgccttc gatattggta aatgtcgagg tgttaaaacc
    15481 ggtggcctgc gcggcagtgc tgcttaagtt agccgtcacg cccaccgtcg aaagccggaa
    15541 atcaaccacg tccatcccgc cgttcgccag ccacgttccg gtgccggagc tgtacaccca
    15601 gccgccggaa ccgtcgtaag tgtcggtgcc tgcggtggcg aagaaggtgt cgttatagcc
    15661 ggtgccgacg aacttgccgt tgttgctcgc ggtacccgct tcagcgctga gatcgtaagc
    15721 gtgagtggca tcggtggcct gtaaaccgcc ccagctggtg ttgaccgtct ggtcgatatt
    15781 gctggcggtg gtgccggtgg atttgatcag caccgcgttg taggtttctc cggcattcag
    15841 cccgtagaag ttgaccagcg ccgaggtgtc gttaaccccc atacccgact gcgggttgat
    15901 gatttgcgtg gcgaccagca ctcccgccga gttgtacagc ttcacggtat tgccgtaata
    15961 gacgttaatg ccgttgggat cggtgatgcg caggtgtagc gaagtgccgt agctcaccgt
    16021 gttggtgttg cggctcagga acacgctgcc cgactgtttg gacaccagca catccaccgc
    16081 gccgtcccag tcgtagtcca ttgccgccac gccgctggtg gtaccgctct ggctgccgcc
    16141 gagggccgat tgcgtccagt tgctggcgtt gctgacgtta gtataaagat acgactgccc
    16201 ggtctgggcg atacgggcga tatccatcag gccgtcgtgg ttccagtcca ccgccaggct
    16261 aaactggctg gcgtaggtgg ttgccgttgc gcccaccgcc accgggctac ccagtacgcc
    16321 gttggtgttg aataacagcg agccgtagtt agctcttccc tggctggcgg gcaggaagag
    16381 atccatatcg ccgtcaccat cgaaatcggc ccaggtcatg gaaacgctgc tgctcatagc
    16441 gccactgccc gccccgctca ggaaggtatt ggtggtgttc tgcccccaga caaacgtccc
    16501 attcccctgg ttgatgagcg aagacagcgt gtaatagttg ttcaggttat aggtgtgctg
    16561 aaccaggtcg atcctgccgt cattgttcag atcgacgcca gaaccttcgt tgagcgaaag
    16621 ataccccgtc accgccccgc ccaccgtggc gctaccgccg ctgttcgacg tggtggagtt
    16681 gcctaccagc gtgcctgcgt tgttccacag gaaggtgttg gagtccggcc cgccagcgtc
    16741 accaatccag aagtcgagat agccgtcgcc ctctttatca aatgccacaa tcgagccgta
    16801 ccacgtcagg gtgccgacgg tgacctgaat agcgctggaa aaggtgccat cgccgttatt
    16861 ggtaaagccg ttaatatagc ccgtaccgta gtcatcacgg gtgataaaca gatcgcccgt
    16921 gccgttacgg tcgtaatcgg caatggagct ggtggcgtag ttgttgccgc tctgatacag
    16981 cgtcagcgcg gtcttcgacc aggtgagtgg gtcagtcgat tgcattacct gctggtttgc
    17041 cagcaccgtc cacatcccgt gcgagtcgag gccgtaggtc agcccccagg cggtggtttt
    17101 gctggcggta gtccaggtcg gtgtgtaatc aatcgccgcg ttaaccgtca ccgtgccgtt
    17161 gctaatattg gcgttattgc cgttacccgc cgaacttttt acctgcgcag taacggtata
    17221 ggcggtcgcg gaaactgtca gcgcatcggt atccggcaac tgtacatacc aggtgttgtg
    17281 cgccggatcg accactaccg cgccgcccgg ttcagaggtg taggttttgc cgttgatcac
    17341 cacttcaaca tactgcccac tggcaagcgc ggcggtgatc accccgctaa taatcggcgt
    17401 agtatcgcgc gtggtctggc tggtgatttg cgccagcgtg gtcggaatag aggtatcgac
    17461 cgtcaggaca aaatcactgg aggaggtgat attccccgcc aaatccacca ctcgcgcgct
    17521 gtaggtatag gcaccgctca ccagcccgct gtcgctgtag gtccagttca gagcgccgac
    17581 catcgtcacc gcccctaaca gcagcccgtt acggtagagg taaaccactt caccgctggc
    17641 aagcggcgcg gaaagtacac cgttcagcag cggcgtagtg tcgtcggtgg cctgcgaact
    17701 gcttaatgtc ccctgccgct gcccgacatc atcggtatac gacacaatcg ttggtgtggc
    17761 ggtggggtta atcgtatcga ccaccaccgt tttcgacacc accgggccgg tgttccccgc
    17821 cagatccagc acccgcacct gataaacgta gctgccgtcg gtcagcgtgc ggctgtcggt
    17881 gtaagtccac tgtgtaccgt tggtggtgag cgttgtccag gtcgcgccgc tatcaaggct
    17941 aatctgcgcc acttcgccgc tggcaagccc cgcccccagc gaaccggtca gcgtgagcat
    18001 ggtgtcgctg gtgataaagt cagttgccga actccccgtg tcggtgctga tgctggcgat
    18061 ggtgagcacc tgcgccgggt taacggtatc gatcagcgcc gactggctgc tcgttgcgcc
    18121 aacgttgccc gccagatcga ccacccgcac ctgccaggtg gtggtgccgt tggtgagggt
    18181 tcgtccgtca acgtaactcc agttcaggct gtctgcggcg acggtcacgt tcacccaggt
    18241 tgcgccgttg tcggtactga tttgcgcgaa ctcattagcg ccgagcgccg cgcccaatac
    18301 gccgcgcacg gtaagcgtgg tgtcgctggt aataaaatcg ctgctgctgg ttccggtgtc
    18361 atcgctgata ccggtaatgg tgatactttt cgccgcttcc gggctggtgg tgtcgaccac
    18421 cacgttttgc gttgcggtct gcccaacgtt ccccgccaga tccaccaccc gcaccgtgta
    18481 gttccaggtg ccgtcagtaa gcgtgtgacc atcggcatag ctccagctgg tgccaacgac
    18541 ggttaacgta gtccaggtga cgccgccgtc gaggctgatt tgcgcgaact caccggcaga
    18601 aagcgtcgcc cccagcgtgc cgctcaccgc aagcgtcgtg tcgctggtga caaaatcgtt
    18661 agtgatcaag cccatatcgg tggtgatcgc gctgatggcg atggttttca ccgcgggatc
    18721 tggcgcggta gtgtcgatca ccacattctg gctgtcggtc gcgccaacgt tgcccgctgc
    18781 gtcaatcacc cgcacctgat agagatagtt gccatcggtc agcgtgcggc tgtcgttata
    18841 gcgccaggtc gtgccggtaa cggtaagcgt ggtccaggtt gtgccgccgt cgatacttat
    18901 ttgcgccgtc tcgttactgg caagcgcggc ggtgagcgag ccgttgacca ctaccggact
    18961 gacgctggtg ataaagtcgc tggcgctaag gccagtgtcg gcttgcaggg aatcgatatt
    19021 aatgcccatc gccggagcgc tgagatcgac ggtaatggtg tgattactgc taccggtgtt
    19081 gccaatggca ttgctcaccg aggcgttaat cacgtaggcg ttgccgtccg ccagcgcggt
    19141 gacatcggcg ctgccgaggg tgtagctcca gctaccgccc gtctgcaccg tggtggtgta
    19201 ggttttgccg tttaaggtca gggtcagcgt ctgcccgacg ggcgcggtgg tggtgccgct
    19261 gatcaccagc gatgagccgt gttccgctgc attcaccaca tcgtcgcccg caaaggtgtt
    19321 gatggtgaca gtaggtacat cgccgttcag cgtcacgcca cggctggcgc tgccggggtt
    19381 tccggcctga tcgctaaccg tcgcgctaat ggtgtagctg ccgtcgctta atccggcaaa
    19441 ctgctgcgcc ggaataaaca ccgaccagct tccgccgctg ccaaccgttg cgctccaggt
    19501 ttgcccgtta aaggtaacgg tcaccgtctg gcccacttcg gcggtggtgg tgccgctaat
    19561 ggtctgcccg gcaagctgct cggcgttgtt gataatatcg tcacccgcca ccgtggcgat
    19621 ggtgaccgtt ggcgcaacgg tatcgacggt cagggcgtgc gtggcctgtc cggggttacc
    19681 ggcgcgatcg ttcacgctgg cggtaatggt ctgcgcgccc tgcccgagcg cggccagatc
    19741 caccgcaggc acgttcagcg cccaggtacc attggcggca accgtggcgg tataggtttt
    19801 gccgccaaag gtgacggtga ccgtctgccc cacttccgcg ctggtcgagc cgttaagggt
    19861 taacggctgc tgctgttcgg cggcgttgag gcggtcgtcc gtcgaaacgg tgttaatgct
    19921 gataaccggc gcggtgagat ccaccgccac gctgcgcgag gccgtggcgc tgtttccggc
    19981 actgtcctga gcggaggcgc taaccgtgta gctggtgcca tcactgagtg ccgcaacatc
    20041 cgccgcaggc acgttcacgc tccagctccc gttgctctga atggtggcgc tgtagccttt
    20101 gccgttaagc aacacggtca ccactgtacc tgtcgcgaaa ttagtgccag tgccgctcag
    20161 ggtcagcgca ctgcccgctt cggcggcgtt aataaggtta tcgccgctga tagtgctgac
    20221 cgagagcgac actgccgccg tgttcacctg tacgttgtgc gtctgcgtgc tgctgttgcc
    20281 tgcgctatcg gtaatggtgg cgctgatggt caccgtgcca tccgcaaggc cggaaatcac
    20341 gctggcaggc acgccgacgc tccagttgcc gctggcatcg gtgctggtga catactgctg
    20401 cccggcgatg gtcaccacca ggcggtcgcc cgccaccgcg cccgtggcgg tgccgctgat
    20461 aatctgcgcc tgaatgtgtt cgacgttgtt aatcacatca tcgcccgcca cggtgttaaa
    20521 actgatcacc ggcgcagtgg tatcgacggt cacgcctttg ctggcgctgc caaggttacc
    20581 cgccagatcg ctgaccgtgg cggtcagggt gtagccgtta ttagcaagtg cggttaagtc
    20641 gctggcgggc agcgttaaac tccagctgcc gtctgtctgc accgtggtct ggtagttagt
    20701 gccgttaagc gtgacggtga gcgtctgccc ggtttgcgcg gtgctggtgc cggacaccgt
    20761 tacgcccgcc gccgcttcgc tggcgttgat gatgttatcg ctggcaatgg tgttgatggt
    20821 caccgttggc gcggcgctat cgacgctgta ttcgtgcgtt gctgtggcgc tgttgccgtt
    20881 gacgttagtc acccgcacct gggcgctggc atcgccatca cgcagggccg ccaaatccgc
    20941 cgccggaacg gtggtgctcc agctaccgtt tgccgccacc gtggtggtgt aggttttgcc
    21001 gccaaaggtg acggtgacgg tttgccccgc ttcgacgccg gacgtgctgc cggagagcgt
    21061 taacgccgcg cctttttcgg cggcgttaat cgcatcatcg ctggtgatgc tgttcatgct
    21121 gatggcgacg gcggtgagat caacagtcag cggatgggta atgctggttt gcgttccggc
    21181 actgttcgcc ccgctaacgg taatattcag cgttcccgca ggccagttac tgacatccgt
    21241 cgcaggaacg ccgacgctcc agctaccgtt cgccaacacc gtggcggcgt aagtttggtt
    21301 gttaatcacc accgtcaacg ccgtgcctgc ggcaaagccg gagctgctgc cggtgatcac
    21361 ctgcgcctga gcgtgttcga tgatattgac cacatcatca cccgcgacgg tatccacccg
    21421 caggccaggc agactggcat cgatggtgat atcgcgcgtg ccgctaccgg tgttgccaac
    21481 ggcgttggtc accgaggcat taaccgttaa ctcaccgtta cccagagcct gccagtcggc
    21541 ggcaggtacg ctcacattcc agcttaaatc agcctggacg gtggcggtgt aagttttacc
    21601 gcccagttca acggtgacgg tgctgcccgc cgccgcaccg gttacctggc cgctgatagt
    21661 ttgcgcgctg cctgcctctg aagcgttgat aatatcgtcc gtcgccaccg gattaatggt
    21721 gatgcccggc agcgcggtat taacctgcac gttatggctg gcggtgctgc tgttaccctg
    21781 agcattggtg acgctcgccg tcacgctgta ggtcgcttcg cccagcgcac tgaccgccga
    21841 ggcaggcacg gtaacactcc agttgccgga ggcatccgtg gtggcgctgt agttttgtcc
    21901 gttcagggtg acggtgatcg ccgtacccgc aggctgatcg ctggtgccag aaagcgccag
    21961 atcagatcct ttttccgtcg cgttgatcac atcatcgccc gcgatggtgt tgatgctaat
    22021 cactggcgcg ctgaggctga cggtcacggt gcggctggcg tcgtcgctgt tgcctgcccg
    22081 atcgctgacg ctggcggtga tggtctgcgc accgctaccc agcgccgtaa catctgccgc
    22141 cggaacgcca acgctccagt tgccggaggc atccagggtg gtggtgtagg ttttgccgtt
    22201 gagcacaacg ctcaccacat cgcccgcttc cccgccgcta ctggtgccgg agatcaccag
    22261 cgcctgcgca tgttcggcat cgttgatgat gtcatcgccc gccacggtgt taatggtgag
    22321 aaccggcgcg gtggtgtcta ccgccagatt atggctggcg ctgccggggt tgcccgcttt
    22381 gtcattgacc gtggcgctga cggtgtaatt gcttgcgttg agcgcgccca gggctgaagt
    22441 gggaacgctg acgctccagc taccgtccgc ctgcacagtg ccggtgtagg ttgcgccgtt
    22501 aagggtgacg gtcaccgtct gcccggtttc ggcggtgctg gtgccgctga tggtgagcgg
    22561 gcttccggct tctgcggcgt taaggatatc gtcgctggcg atggtgttaa tggtgagcgt
    22621 cggcgcgctg gcgtcaacgc tatacgcatg ggtcgccgag gcactgttac cgctcgcgct
    22681 gctaacgctg gcctgcacat tcgccgcgcc ctctggcaga gttgccagat ctgccgccgg
    22741 aacgtttaca ctccagctcc cgttggcggc gacgcttgca gtgtaggttt taccgccaaa
    22801 agtgacggtg acggtttgcc cgctctcaat cccgctggta ctgccggaaa gagtcagatt
    22861 ggtgcctttt tctgcggcgt taatcacgtc gtcgctggca acggtgttga tgctgattgc
    22921 caccgccgtt aaatcgacgg tgaacggatg gctgacgctg actgggttat tggcgctgct
    22981 ttgcccgtct acctccacgg ttaacggccc cgcaggccag gcgctgacgt ttgccgccgg
    23041 aatgccaacg ctccagcttc cgtccgcctg caccgtggcg gaatacgcca cactgttgat
    23101 ggtaaccgtc agcacagcac ctgcattcag gccgctactg ccgccggtga tcaccagcgc
    23161 ctgcccgtgc tcgatgctgt tgacgatatc atcgcccgcc acggtatcta cgcgcagccc
    23221 cggcaggttg gcatcaatgg tgatatcccg cgtgccgctc ccggtgttgc cattagcgtt
    23281 ggtgaccgag gcggtaatgg tcaaatcacc attgcccaac gcctggagat cggctgtcgg
    23341 aacgctgacg ctccagctta agttgctctg taccgtagcg gtgtaagtgt tcccgcccag
    23401 cgtcacggtg accgtatcgc cagcggcggc gcgagtcacc accccgctga tggtttgatc
    23461 ggcccccgct tcggcggcgt tgataatatc gtcgcctgcc acggtgttga tggtgacgtc
    23521 cggcaggccg ctgtcgacca gtaaattcgc ctggctgctg gcggtgttgc ctgctgcact
    23581 ggtggcgctg gcgctgaccg tatagttggc ctgacctaat gccgccagat cgctcaccgg
    23641 cacggttaag gtccagttgc ccgatgcgtc ggtggtggcg ctgtagttgt gaccgttgag
    23701 cgtgacggtg acctgcgcgc ccgtcgccag ccccgtactg ccgccgctga tggtcaacgg
    23761 ctggcctttt tcatcggcgt tcaggatgtt atcgccgctg atggcgttaa aggtaatggt
    23821 cggcagcccg gtattgaccg tcacctgatg ggtagcgctg ccgctgtttc ctccggcatc
    23881 ggtgacgctg gcattgatgg tcaccgtgcc attcgccagt gccgagacga cgcttgccgg
    23941 aacgccgacg ctccagttgc cgctggcatc cagcaccgtg gtaaaggtat ttgtgccgat
    24001 agtcaccgtc acggtgctac cggttgccgc tccagtggcg gagccgctga tgatctgcgc
    24061 ctgggcgtgt tccgtcgcgt tgatcacatc atcgcctgcc accgtgttga tggtgacgac
    24121 cggaacggac gtatccaccg tcaggttgtg attaaccgag gccgggtttc cggctttgtc
    24181 gctcaccgcc gcgttcacgg tgtagttgct ggcggtcagg gtgcttaagt cggctgacgg
    24241 tacgctgacg ctccagctgc cgtccgtctg tacggtgccg gtgtagtttg tgccattgag
    24301 cgtgacggtc accgtctgcc ccgcttccgc cgtgctgctg ccggtgatgg tcagagccgc
    24361 tccggcttcg gcggcgttaa gaatatcatc gcccgcaatg gtattaatgg tcaccgttgg
    24421 cgcgctggca tcaacgctgt aagcgtgggt cgtggtggcg ctgttgccgt taacattgct
    24481 gacgctggcc tgtgcgctgg catcgccatc acgcagagcc gccatatctg ccgccggaac
    24541 cgaggtgctc caggaaccat tcgcagccac cgtggcgctg taagttttgc cgccaaaggt
    24601 gacggtaacc gtttgtccgg cttcaacgcc agaggtgctg ccggagagcg ttaacgccgc
    24661 gcctttttcg gcagcgttga tcacatcatc ggcggtaatg gcgttgatgc tcaccgccac
    24721 cgccgagaga tcgaccgtca ccggatgcgt aacgcttacc ggatttccgg cagaggtgct
    24781 accgctcgcc gcaatcgtca ccgatcccgc aggccaggcg ctgacatcca ccgccggaac
    24841 gccgacgctc caggtgccat ccgccagcac cgccgcaaca taggtttgcc cgttaatggt
    24901 cagcgtgacg ttgctgcccg ccgccagacc ggagctgctg ccggtaatca ccagcgcctg
    24961 cccgtgctcg ataatattaa ccacatcatc gcccgccacg gtgtcgaccc gcagaccggg
    25021 gagattcgcg tctatggtga tttcgcgcgt gccgttacca gtattgccta cgctgttcgt
    25081 caccgaagcc gaaatggtca gttcgccgtt gcccaactcc tgtagcgcgg aggccggaac
    25141 atcgacgctc cagcttaaat tcgcctgcac cgtggcagtg taagtcgccc cgccgagcgt
    25201 gacggtcacc gtgtcgcctg ccgccgcacc cgtcacctgc ccgctgatgg tctgttccac
    25261 gcccgcttcg gcggcgttaa taatatcgtc cgttgccacc acgttaatgg tgacgcccgg
    25321 cagcgcggta ttaacctgta cgttatggct ggcggaaccg ctgttgccat cggcgtcagt
    25381 ggcggctgcg gtcaccgtgt aggtggcttc accgagggcg ctcacccgtg acgccggaac
    25441 ggtcacgctc cagttaccgg aagcatcggc agtggtggtg taattttgcc cgttgagcgt
    25501 aacggtaatc tgcgtgcccg caggctggtt gctggtgccg gtaatcgcca gatccgcgcc
    25561 tttctccgcg gcgttgatga tgtcatcgac ggcaatggtg ttaatggcga gcaccggagc
    25621 gccgagcgcc acggtgacag tgtgcgaggc cgtgccgctg ttgcctgcgg agtcggtgac
    25681 cgtagcggta atggtcacat cgccctgcgc cagcgcggaa atcacgctgg caggcacgcc
    25741 gatgctccag ttgccgttgg cgtccagcac ggtggtatag gtggtcgtgc caatcgtcac
    25801 ggaaaccgta ttaccggtag tcgcgcccgt tgccgagccg ctgatgatct gcgcctgtcc
    25861 gtgttccgtg gcgttaatga tgtcatcgcc cgccaccgtg ttgatggtga cgaccggagc
    25921 ggcaaggtcc accgtcagat tatgcgttgc cgaagccgaa tttctggctt tgtcgctgac
    25981 cgaggcgtta acggtatacg agctggcggt aaggctagct aaatcgccgg ttggtacgct
    26041 gacgctccag ctcccgtctg cctgcacatt gccgctgtaa ttaacgccgt ttagcgttac
    26101 ggtcaccgtc tgcccggctt ccgccgtgct ggtgccgctg atggtcagcg ccgatcccgc
    26161 ttcggcggcg ttaagaatat cgtccgtggc gatggtgttg atggtaacgg agggcgcagt
    26221 ggcatcaacg ctgtaagcat gcgttgcctg ggtgctgttc cctgcgacat tgctgacgct
    26281 ggcctggacg tttgctgcgc cgtctggcaa ggttgcaaca tcgacggcag ggatcgtcag
    26341 cccccaggta ttatccgcgg caaccgtggt ggtgtagctt ttgccgccga agataacggt
    26401 gatggtttgc cccgcttcca cgccggaggt ggtgccggag agctgcaaat cagagccttt
    26461 ctcggcggcg ttaatcacat cgtcagtgga aagggtgttg atggtgatcg ccacggcagc
    26521 gagatcgacc gtgaccggat ggctaatgct ggttgtggtt ccggcggtgt tggtgccaga
    26581 gacggcaata ttgaccgtcc ccgcaggcca gtttgtgaca tctgccaccg gaacaccaac
    26641 gctccagcta ccatccgcta aaaccgttgc gccgtaggtg acgctattga taaccaccgt
    26701 caacgccgcg cccgccgcca gtccgctact gctaccggtg atcaccaacg cctgagtgtg
    26761 ctcgatgctg ttaatcacat catcgcctgc cacggtatcg acgcgcagac ctggcaggtt
    26821 ggcatctatc acgatatcgt gcgtcgcggt gccggtatta tttgcggaat tggtcaacga
    26881 ggcgctgatg gtcagttcac cgttacccag cgcctgcaaa acgttcgccg gtacgctgac
    26941 gctccagctt aaatctgact gcacggtggc gttatattga ttgccgccaa tggtgacaat
    27001 caccgtattc ccggcaaccg ccgttcccgt gacctgaccg ctgatggtct gcgccacaac
    27061 gatttcagcg gcgttgataa tgtcatcgcc ggagacggtg ttaatggtca gcgtcgggcc
    27121 gctaagatca acggtcacat cgtgagtcgt gctgtcgctg ttgcccgcgc gatcgctcag
    27181 gctggcggtg atggtctggc taccggtcgc cagcgccgtg acatccgccg ccggaatgcc
    27241 cacgctccag ttaccggagg catccagcgt ggtggtgtag tttttaccgt tcagcgtgac
    27301 ggttaccaca tcacccgccg ccgcgcccgt actggtgccg ctgaccacca acgcctgacc
    27361 atgttcgatg gcgttgataa tgtcatcgcc cgcgacggtg ttaatggtga gatccggcgc
    27421 agtgatatct accaccagcg cgtggtcggc gcttgccggg ttacccgctt tgtcgctcac
    27481 cgtggcggtc acggtgtaac tgctggcggt taatccgctt aaatccgctg ccggaacatt
    27541 cacgctccag gtgccatccg ccagaacggt ggtctggtac gtgttgttat tgagcgtgac
    27601 ggtgagcgtc tgcccggcct gcgccgtggt agtgccgctg atggtgatcc ccgcgccagc
    27661 ttcgctcacg ttaaggatat cgtcgctggc gatggtgttg atggtcacaa gcggcgcggt
    27721 ggcatcaacg ctatacgcgc gatcggcctg ggcgctgttg ccgttaatat tactgacact
    27781 cgcctgcacg ttgcccgcgc cgtccggtag cgcagcgaga tcggcaggcg gcacattcac
    27841 cgtccagcta ccgttagctt ccactgtggt ggtgtaattt ttgccgccaa aggtgacggt
    27901 caccgtctgc ccggcttcga caccgctggt gctgccgctt acgaccagcg tttcgccttt
    27961 ttcgacggcg ttgatcacat catcaccaga gagagtgtta atggtgatgg cgaccccagc
    28021 cagatcgacg gtaaccggat gggtaatggt ggaggttgtt ccggcactgt tggtgccgct
    28081 taccgtgata ttcaccgtac ccgctggcca gttgctgacg tctaccgccg gaacaccgag
    28141 gctccacgtg ccgtcggcta ataccgtcgc gccataagta acgttgttaa tctcgaccgt
    28201 taacgccgtt cccgccgtta atccgctgct gctgccggtg atcaccagcg cctgattgtg
    28261 ctcaatgctg ttaataacat catcgcccgc cacggtatcg acccgcaggc caggcagatt
    28321 ggcgtcgatc gtgatatcgc gcgagccgct gccggtgttg ccgacgccat tggtgaccga
    28381 ggcattaacc gtcaggtcgc cgttgcccag cgcctgaata tccgccgccg gaacgtccac
    28441 actccagctt aaattagact gcaccgtggc ggtgtaggtg ttgccgccca gcgtaacggt
    28501 aaccgtatcc ccctgcgctg cgcccgtcac ctgcccgctg atggtttgcg catttcccga
    28561 ttcggcagcg ttaataatat cgtcagtcgc caccgcgtta atggtaacgg caggcaaggc
    28621 gctgttgacc agcacattat gactggcgga attactgttg cctgccgtat cagtgacgtt
    28681 tgccgttacc gtgtagttgg cttcacccaa tgcgctaacc gctgacgctg gcaccgtggc
    28741 gctccagttg ccgttgctat cggtagtagc ggtgtaattt tgcccgttca gcgtcaccgt
    28801 aatggtggtg cccgcaggct gattactggt gccggtgatt tgcaggtccg cgcctttttc
    28861 cgtggcttta atcacgtcat cggtggcgat ggtgttgata ccaatggttg gcgcggcgag
    28921 attcacggtg accgtgcggc tggcgtcatc gctgttgcct gccgcgtcgg taattgccgc
    28981 agtgatagtt tgcgggccgc tgccaagcgc agtgacgtca gccgccggaa cgccgacgct
    29041 ccagttgccg gaagcgtcca gcatggtggt gtaggtttta ctgtttagtg tgacggtgat
    29101 gacatcaccc gcttcgccgc cagtgctgga gccggagatc accagcgcct gtccgtgttc
    29161 ggcggcgtta ataatgtcat cgccggagac ggtgttgatg gtcagcaccg gcacggtgag
    29221 atccaccgcc agcccgtggt tagcggacgc cgggttaccc gctttatcgc ttaccgaggc
    29281 actaacggtg tactggctgg cggtcagatt gctgagatcc gccgtcggta agctgacgct
    29341 ccagctgccg tccgcctgga cggagccgct gtaagtcaca ccattaagcg tgacggttac
    29401 cgtctgcccc gcttcggcgg tgctgctacc gctgatggtt aacggattgc ccgcctcggc
    29461 agcgttcaga atatcgtcgg tggcgatggt gttaatggca agcgtcgggg ccgtggcatc
    29521 gacgctgtag gcgtgggttg ccgaagccgt gttgccgtta atagtgctga cgctggcctg
    29581 cacggtggcg tcgccgtcgc gtaacacgct gagatcggcg gcgggtacgg tggttgtcca
    29641 gctaccatcg cccgctacgg tagcggtgta ggttttcccg ccaaaggtaa cggtgaccgt
    29701 ttgccccact tccacgccgg aggtgctgcc ggaaagggtt aaatctgccc ctttttcggc
    29761 ggcgttaatc acatcgtcgc cggaaacggt gttaatggaa atcgccaccg ccgccagatc
    29821 gacggtgacc ggatgggtga tggtggatgt tgttccggct gtgttagtgc cacttaccgt
    29881 gatattcacc gtacccgcag gccagttact cacgtctgcc gccggaacac caacgctcca
    29941 tgtgccatcg gctaatactg ttgcagcgta agtgaccgtg ttaatcacca ccgtcagcgc
    30001 cgcgcccgcc gccagcccgc tgctgctacc agttatcacc agcgcctgag cgtgctcgat
    30061 gctattgacc acatcatcgc ccgccacggt gtcaacgcgc agacctggca ggttggcgtc
    30121 gatagtaata tctcgcgaac cgctgccagt attgccaacg ccgttggtca ccgaagcgtt
    30181 aaccgtcaga ttgccattgc cgatggcctg aatatccgcc gccggaacgt ccacgctcca
    30241 gcttaaattc ccctgcacgg tagcggtgta agttttcccg ccaagcgtta cggtaaccgt
    30301 atcgcccgcc gccgcccccg tcacctggcc gctgatggtt tgcgcattgc ccgattcggc
    30361 ggcgttaata atatcgtcgg tcgcaactgg gttaatggtg acgccaggca gcgcggtatt
    30421 gacctgcacg ttatggctgg cggaattgct gttgcctgcg ctgtcggtga cgttcgccgt
    30481 caccgtgtag ctggcttcgc ccagcgcacc tactgcggaa gctggcaccg tagtgctcca
    30541 gttgcctgca gcgtccgtcg tcgcggtgta attttgcccg ttaagcgcca gcgtaatcgt
    30601 ggtgcctgct ggctgattac tggtgccgga aatttgcaga tccgcgcctt tttcgctggc
    30661 gttaattatg tcatcggtgg cgatggtgtt gatgtcaatg gttggtgcgg tgaggttaac
    30721 ggtgaccgtg tgtgtctaat tgtcgctgtt gcctgccgca tcggtaaccg tggcggtgac
    30781 agtttgcggg ccgctgccaa gcgccgtgac atccgccgcc ggaacgccga cgctccagtt
    30841 gccggaggcg tccagggtgg tggtgtaggt tttactgttt agcgtgacgg tgacgacatc
    30901 ccccgcttct ccgccggtgc tggaaccgga aatcaccagc gcctgcccgt gttcggtggc
    30961 gttaataatg tcatcgcccg caatggtgtt gatggtcagc accggaacgg tgagatccac
    31021 cgccagatta tgcgtggtgc tcgccggatt gcccgctttg tcattgaccg tggcggtcag
    31081 ggtgtggctg ccatcggtca atgcctcaag atctgccgcc ggaatattga tgctccagct
    31141 gccgtccgcc tgcaccgttg cctgataggt ctgcacggtc gggctgttaa gcgttatcgt
    31201 aacaatctgc cccgcttcgg cggtggtact gccgctcacc gtcacgcccg catcggcttc
    31261 gctggcgttg acgatattgt cgctggcgac ggtattgata atgatggttg gcgcgctgct
    31321 gtcgacgctg tagttgtgca ccgccgaggc gctattgccg ttgatattgc tgacgctggc
    31381 cagtgcggag gcgctgccct cgggtaatga cgccagatcg gcggcgggta cggtggcagt
    31441 ccagctacca tcgctcgcta cgctggcagt gtaatttttc ccgccaaagg tgacggtgac
    31501 ggtttgaccg ggttctacgt tagtggtggt gccggaaagg gttaaatcag cgcctttttc
    31561 tgcggcgtta atcacatcgt ccgtggcgat ggtgttgatg gtgatcgctg ccggagtgag
    31621 atccaccgtc accggatgcg taatgctcac cgagtttccg gcgctacttt cccctgaaac
    31681 ggcaatatta accgtccccg caggccaggc gctaacctgc gccgccgtga cgcccacgct
    31741 ccagctacca tcggcctgca ccgcagtggt gtattccaca ttattaatcg taacggtaag
    31801 cggcgtgctt tcagccagcc ccgagctgct gccggtgacc accagcgcct gcccgtgctc
    31861 gatgatattg accacatcat cgcccgccac cgtatcgacc cgcaggccgg gcagattggc
    31921 gtcgatggtg atatcccgcg tgccgctgcc ggtgttgccg ttttgattgg tgactgaggc
    31981 attaaccgtt aaatcgccat ttcccagcgc ctgaatatct gccgctggaa cgtccacgct
    32041 ccaggtgaga ttgctgccca ccgtcgccgt ataagtatta ccacccaacg taatagttac
    32101 cgtgtcgcca tcttccgcgc cagtcacctg cccgctgatg gtttgcgcca cgcccgcttc
    32161 ggcggcgtta ataatatcgt cggttgccac cggattaatg gtcacaccgg gcagcgcgct
    32221 gtcgaccagc acgttatggc tggcagtggc gctgttgccg atatcgctgg tcaccgccgc
    32281 cgttaccgta tagttggcct ggcctaatgc tgtaaccgcc gatgccggaa cggtgacgct
    32341 ccagttgccg gaggcgtcgg tcgtggtggt gtaattttgc ccgttcagcg tcacggtgat
    32401 ggcggtgtta acaggctgat cgctggtgcc ggtaatctgg agatcggccc ctttttcgct
    32461 ggcgttaatc acatcatcgc ccgcaatgct gttaatgccg ataaggggcg cggcggtatt
    32521 gaccgtgacg gtcaatgact ggctgcccgt gtttccggct ttgtcggtta ccgagacgct
    32581 gacaggataa ctgccgtccg ccagcccact gaccaccgag gccggaacgc ccagactcca
    32641 gttgccggaa ccatccacca ccgtggtgta atccacatta ttcagcgtca cggtgacgat
    32701 atcgcccgcc tgagcgccag taaccgtgcc gctgatgatt tgcgcctgag catgttccgg
    32761 cgtgttgatg acgttatcgg tcgccaccgg attaatggtt accaccggcg gcgtggcatc
    32821 caccagcgcc acgcgtgaaa cgctgctcgg attaccggcg acatcgttca cgctggcggt
    32881 gaccgtagcg ttgccgtcag ccagtgcgcc cacgttggcg gcgggcacag tcacgctcca
    32941 ggtgccgtct ggctgcacga cgccctgata cgtctgccca tttaacgtaa cggtcaccgt
    33001 ctgccccggc tgagcgtcgg tggtgccggt gatgggcaga tcctgcccgc tttcggcggc
    33061 gttaatgatg ttatcagcgg tcagtggatc aatgcttaag gccggggcct ggctatcgac
    33121 ggtaatggtg cgggaagtgt tgccggtatt gccgctgaga tccgtcacag aagcggtaat
    33181 cgccaccgcg ccgtcaggca gatttcccat cgcggcggca ggtaccgtca gactccagga
    33241 gccatcggcc tgcacggtag tggtaaaact ctgtccagcc agggttactg tgacggtttg
    33301 tcctgtctca gcctgttgag tgctaccggt aagggtcagt ggcgcacctt tttctgcgcc
    33361 actgataatg tcatcgccgg agacggtgtt aatggtgatc aacggagaaa caccgaccag
    33421 cgtgattgct cgagacgccg cgccagtatt tcctgcagca tctgtcacgg tgacgaaaat
    33481 cgtattggct ccgcgatcca gcgtgcgggt taccgctggg tccagcgcca cattccagct
    33541 gccatctgcc agcacgatgc ccgtcaggac atgagtgccc aatttcaccg ttaccgtatc
    33601 gcctggagaa gcaccgctga cttgtccgga gatgatctgc gcgacggcct gctcggcatt
    33661 attaagaata tcgtcgcccg ccacggtatt aacactgacc acgggtgctg aggtgtctac
    33721 cgtgaaattc gcgctgtggc tggtggtgtt ccctgcccga tcgctgacac tggcattgac
    33781 agcgtaatta ccctccgcca gtgcctggac ttccgtggca ggcagcgtca cttgccaggt
    33841 tccgtcgcta ccgacggttg ctgtatggct tttcccgttg acggtcagtg ttacggtttg
    33901 ccccgcttct gcattcgacg tgccgctcag taccagcgcg acgttatgtt ccgcagcact
    33961 gataatgttg tcctgagcga tggtgttgat ggcaagcgtt ggcggttggg tatcgaccgt
    34021 tagtagctgc gcgccggtga ccgtattgcc tgtggcgtct ttcccactga ccagcaccgc
    34081 ctgattccct tcgcctaacg cctgtgcatc ggcggtcggc acctgcacgc tccaggcacc
    34141 gttagcgcct acgctggtcg tgtaggtttt gccgtttagc gtaacggtga gttctgttcc
    34201 ctgcgccaga tggctactgg tgccagaaag cgtaaacccg gcggcctgtt cgctggcgtt
    34261 aataatgtta tcgcctgcgg tggtggcaac ctgcacgctg gcctgactat tgtcgatggt
    34321 taacgttatc gaaccggcgt cagtgatggt actgccgaca atttgcacca ctgaccaggt
    34381 atgttcacct gcactttgcg tgggtaacga gacactccag tgaccgttgt tgtctacctg
    34441 agtactggca atagtattgc cgttactgtc tttaatctga atagtcgccc ccgcctgccc
    34501 gctgccgcta aaggttggcg tggtgtcatc ggtgatgtca ttggtggcta gaatgccgcg
    34561 ctgatcgccc tgattatcgg taactaaaaa tgatggtgtg gcgttgccag gctcagcagg
    34621 tggcgtaggg ttattaatca cttctgtttt cgagtcgcca tcgccaccgc ttgccagcag
    34681 tgcaccaagc gcgccaccac ctaccgccgc ccccgccagc caaccccacg ggaaggcgct
    34741 ggtctgagca acggtatcaa gaaatggcgc aatgctttca atcgcagtgg tctgggcagt
    34801 cagttctacg ggggctaatc cacccgcagc agtatcggca aaggtgatat gggttagctg
    34861 ttgaccatcg gcaaacacca gttccgattg ttcagctgta ttcgccgctt gcaggaaata
    34921 accgttgcag cggatcaccg tgccgtcctg catatagatc agcaggtcat ttccttcacg
    34981 gacgtaacgc gcaacggcag aggcctgagc atgaacctgc accacggaag gctcataaat
    35041 aacaaccgtg acgtcgccag gattaatcag cgtttgtttg acggatgttt tacgcgaaat
    35101 aacatcaata atcaggctca taatggcatt cctgataaat ggcgcacatc cttagcgagt
    35161 ccatttatct aaataagaca aaggtaatca cttaacaacg gcccactatt aataatggca
    35221 ggattatttc aagtcgggta agtcgttgta taagccctgt ttaattcccg ctaatggaat
    35281 aaggttattc accgcagcgg cataatttac ggcggcgacc cagccatcat aattggcatt
    35341 tatttcagcc gattgcgcct gaaagacatc ttgttcgacg gttaatagat cattcagact
    35401 gcgtttaccc aatttatatt cattttggta cacatctcgc gttttgtgcg cactttcgga
    35461 ttgcgctaaa ccggcttctt cacgaccccg tgcgccggtc cagttcgcat atgccacaga
    35521 cgctcgttgc agcacatcca gttttgcctg ttcgacctgc gaggcagaga ttttttgttg
    35581 cccttcggcc tgttgcacct gggcagaaac cgcgccgccc tgatacagcg gcgcgttaac
    35641 gttcagttgt agctgatcat cccaatacga gcggtcgctg gtctggtagc gcgttttacc
    35701 cccctgaata ctgagcgttg gccagtattg cgctttcgtt ttttccacgc cgtactgtgc
    35761 tgactggcgt aagttttctg ccgccagcac cagcgggatg gactggtaat caatattctt
    35821 cagcgatacc ggctgctcgg ctaattcagc aggtggcgcg gctatcgcct ccggctgtac
    35881 gccagtgagc accgccagtt gcgctttggc gctcgccatc tgcgcctgat attgctccag
    35941 cgttgagcgc attccggcaa tacgcgtctg ggcctgtaat tcatccgacg atgagttcag
    36001 gcctgcgtta gcgcgcaatg ccgccatgtt gtagacgttt tccagcgagt gaatattgcg
    36061 ttgagccgca tcgcacaaag cctgataacg actgacttcc atgtaggcag tcgccgtttt
    36121 ctctgcaaca tcggttaagg ttgccatcaa tttaaagcgg tagctgtcac gggcggcagt
    36181 ttgcagattg atatcgttgt tagttttacc aaagtcatac accagttgtg tcagggttat
    36241 gccatacgaa acgttgttat caagcctgcc gctggagtcc gtttgtcggg acggccccgc
    36301 gttgccggtt aatccaacgt gcggatacca ggcacttttg gcttcatcaa tttgcgcttc
    36361 gccaatacct atctgcgccg cctgttgagt gacaccggga tttcgcgcaa aggcgcgtaa
    36421 tatactttcc tgtaaagata attgggcaac tggcgttacc gctggggcat tcgcccattg
    36481 tggcagcggt gcagcaattg cagcttctcc catcaccata aaacttataa aaaaaataag
    36541 tccattattt ttacggtttt taaactggaa tcgcgccata gataagtcac ttccttccag
    36601 gtcattattt taactggttt ttataaccag tctcgtttta ttaagaaggg taataacttg
    36661 tgcatctctt ttgtcgatca ttgatatggg caaagaaatg cagctaaaaa gcttatacgt
    36721 tatttattta gaatacacta cgacttttag attaccatca tacacattaa aaataatcca
    36781 cggtaataag tgattagcat cacagattta tcgcgtatat atcatttttt tgtgacttat
    36841 ttattaaagg aataagaaag gtaatttgaa ttaaagacaa aggcgcagta caggcagtac
    36901 tgcgcgcaga gaggtgttat acggttttat tgcgcttcat gaccattgcc acaataaggc
    36961 tgagtaagat tgcaccaata atccatatca acgcacggct gccctgggac cacaggctgt
    37021 agataaagcc aacgatcacc aacaacatca ccaacgtacc tacgataaga atcggcaacg
    37081 aggcgtgaat atcatgacgc aggcggatcg ccaccacaaa caccgccaga tagcaaatca
    37141 aaaaggtggc gctggcaacg ctggcgagtg agcctaaatt tagtgccgcc gtcatcagca
    37201 taatcaacac cacgacaatg atgttgcccc aggtactctg ccgccacagg gatttattca
    37261 ttagcttcgg cagttcgcgt tcgctgccca tgttgtccat gatgttaaac acggcgaaca
    37321 ggttcgcgtt gatggctgaa gccgtcgcca gtaaagcgcc gatgacgacg atcacataac
    37381 ccacatgccc gagcagcgga gaagcagcct gcgctacggc ggtatcggca tatttttcta
    37441 actctaatgc cgatacatcg ctaagcaaaa ccagcgccag cgagatataa agcaacgtgg
    37501 taacgccaat tgccaccaga aacgcccgtg gcataatgac ctgcggatct ttcactttat
    37561 ccgccgcgtt cgccatcatg ccaaagcccg cataggcaag gaaggttatc ccgatacagg
    37621 agaagaacgc accggagctg gggggcgcag agacggaaat atgcgccggt tgcagcgacc
    37681 agacaccggc aataatcagc aataacagga tcatcatttt aatgccgacg aggatcacct
    37741 ccagccgccc taccgcatgg ttgcttaagg agttgaaaag cgtcatcacc gcaatgatcc
    37801 ccaacgcgta gagcaaaata aggtgctcct cctggctgcc ttcatgcaaa aactgcacgg
    37861 cataagcgcc aaaagcacgg gcgaccatgg cgatgctcac cgccagcgtc aacaggtaca
    37921 gtaacgagag cgccagcgaa aagacgccgt tgcctaatcc gcgacgaaag aagtcgataa
    37981 tgccgccatt gctgggatag ctcgccccca gacgcgcata ggcataaccg gaaaacatcg
    38041 ccacaatacc gccaaaagca aaggcgaccc aggtcgaggc ttccattagc aacgcagcct
    38101 gccccagcag cgcgaagatc cccgccccca ccattgcccc aatgccgatg gaaacgacgt
    38161 tccatagacc gaggggtttg ttaccgttat taccttccgt gttcatcatg atcagccctt
    38221 aaacacgtta tagccgagtt gcttagcgac cgatgccacc attttttgac cgcgaacact
    38281 gttgccttct tcgtccagcg gtggtgagaa cgcggcaatt cccatcactc cagggacgac
    38341 cgccagaata ccgccaccta caccgctttt gcccggtaaa ccaacacgat acgcccagtc
    38401 accggagcga ccatacagcc cttccatcat catttcggcc agaatgtacg gcacgttgtc
    38461 ggcctgaaga acgcgtttat gcgtcaacgg attcacacca cccgccgcca gcgtcgcgcc
    38521 aagcgttgcc agttcaacag tattgatgag cgtggagcac tgacgggtgt acacgtcaca
    38581 ggcttccatt gcatcacaat agagatatcc ggcggagtac agcagccagg caatggcccg
    38641 gttatggaag ttggttgttt gttccgactg gttgacttcg tcagagagcg ctaccagctc
    38701 accagccagt tgctgttgga tatgtaaaat tcgctgccag cgttgttcgg cattttcagc
    38761 gttaatcagg ctggtggtgg caatagcgcc agcatttacc agtggcgaca gcggtttgcc
    38821 gccatgcaac tctaaggcga taactgagtt aaagggcaat ccggtcgggt cagcgccaat
    38881 tttgtcctgt accgcctgcg ggccgacatc ttctaacgca agggctaacg tacagacttt
    38941 cgagatggat tccagcgcaa agcggtaatc actgtcaccc gcactataga cgttgccatc
    39001 gctggtcacg atagccactg ctgccagttg accaggcaca ttcgccagaa agggaatgta
    39061 atcggcattt tgtccgccgt taagtgagtg aaattgggtg taagcctgat ccactgcctg
    39121 ctgtaatttg tttgcatcta acatcttttg ttaactcctt tttatagatg cgggaggtaa
    39181 ttcctcaccc cggtgccgat tttcaggcat cctgatttaa cttagcaccc gcaacttaac
    39241 tacaggaaaa caaagagata aatgtctaat cctgatgcaa atcgagccga ttttttaatc
    39301 tttacggact tttactcgcc tgggttatta atttcttgac cttccccttg ctggaaggtt
    39361 taacctttat cacagccagt caaaactgtc ttaaaggagt gttttatgtc acaaactatc
    39421 gacctgaccc tggacggcct gtcctgcggt cactgcgtta aacgcgtgaa agaaagtctc
    39481 gaacagcgcc cggatgtgga acaagcggat gtgtctatca ctgaagcgca cgttaccggg
    39541 actgccagtg cagaacagct gatcgaaacc atcaaacaag cgggttatga cgcatctgta
    39601 agccacccaa aggctaaacc gctggcggag tcatcaatcc cgtcggaagc actgacagcg
    39661 gtttctgagg cgcttccggc agcgaccgcc gatgacgatg acagccagca gttgctgctg
    39721 agcggcatga gctgcgccag ctgtgtcacc cgcgtacaaa atgcgctgca aagcgtaccg
    39781 ggcgtcactc aggcacgggt aaacctggcg gagcgtactg cgctggtgat gggcagtgcc
    39841 tccccacaag atttagtgca ggcggtggaa aaagcgggct acggcgcgga agcgattgaa
    39901 gatgacgcga aacgccgcga gcgccagcaa gaaaccgccg tcgctacgat gaagcgcttc
    39961 cgctggcagg caattgttgc actggctgtg ggtatcccgg taatggtctg ggggatgatc
    40021 ggcgataaca tgatggtcac cgctgacaac cgcagcctgt ggttggttat cggcctgata
    40081 accctggcag tgatggtttt cgccggcggc catttttacc gcagtgcatg gaaaagcctg
    40141 ctgaacggtg cggcgacgat ggatacgctg gtggcgctgg gtacgggtgt ggcgtggctc
    40201 tattcgatga gcgtcaacct gtggccgcag tggttcccga tggaagcgcg gcatctttac
    40261 tatgaagcca gtgcgatgat tatcggtctg attaatctcg gtcatatgct ggaagcgcgc
    40321 gcacgtcagc gttcttcaaa ggcgctggag aaattactcg atttaactcc gccgacggcg
    40381 cgcctggtta ctgacgaagg tgaaaaaagc gtgccgctgg cggaagttca gccaggcatg
    40441 ctactgcgcc tgacgacggg cgaccgcgtg ccagtagatg gcgagattac ccagggcgaa
    40501 gcatggctgg atgaagcgat gctgacgggc gaacctatcc cgcagcaaaa aggcgaaggc
    40561 gatagcgtcc atgccgggac agtggtacag gacggtagcg ttctgtttcg cgccagtgcg
    40621 gttggcagcc ataccacgct atcaaggatt attcgtatgg tgcgccaggc ccagagcagc
    40681 aagccagaaa tcggtcagct ggcggataaa atctcagccg tatttgtgcc ggtagtagtg
    40741 gttattgcgc ttgtcagtgc ggcaatctgg tatttctttg gtccggcacc gcagattgtc
    40801 tataccctgg tgattgccac cacggtgctg attattgcct gtccgtgtgc gctggggctg
    40861 gcgacgccga tgtcgattat ttccggcgtc gggcgggcgg ctgagtttgg cgtgctggtg
    40921 cgggacgccg acgcgctgca acgcgctagt acactcgata ccgtagtgtt cgataaaacc
    40981 gggacgctga ccgaagggaa gccgcaggtt gtcgcagtga aaacatttgc tgattttgac
    41041 gaagcgcagg cgctgcgtct ggcggcggca ctggaacaag gttccagcca tccgctggcg
    41101 cgggcgatcc tcgataaagc aagcgatatg cagctgccgc aggtcaacgg tttccgcaca
    41161 ttgcgcgggc tgggcgtgag cggtgaagct gaaggtcatg cgttattgct gggcaatcag
    41221 gcgctgttaa atgaccagca agttgatact aaagcgattg aagcagatat ttcagctcag
    41281 gcatcgcaag gggcaacgcc tgtgctgctg gcggttgacg ggaaagcggt agccctgctg
    41341 gctgtacgcg atccgttgcg tagtgatagc gtggcggcgc tgcaacgtct gcataaagcg
    41401 ggatatcgtc tggtgatgtt gaccggggat aacccaacca ccgccaatgc gatcgccaaa
    41461 gaagcaggga ttgatgaggt gatcgccggg gtgctgccgg acggtaaagc cgaagcgatt
    41521 aaacgcctgc aaagtgaagg acgtcaggtg gcaatggtgg gcgacggcat taacgatgcg
    41581 ccagcgctgg cccaggcgga tgtcggcatt gcaatgggcg gcggcagtga tgttgccatt
    41641 gaaaccgcgg cgattaccct gatgcgccat agcctgatgg gcgttgcgga tgcgctcgct
    41701 atttcccgcg caacgctgcg caacatgaag cagaacctgc tcggcgcgtt tatctacaac
    41761 agtatcggta ttccggtcgc cgccggtatt ttgtggccgt ttactggaac actgcttaac
    41821 ccggtagttg ccggagcggc aatggcgctc tcgtcgatta ccgtagtgag taacgccaac
    41881 cggttgctgc ggtttaaacc gaaggaataa gtacgtcatt gcggatgcgc tgctttaggg
    41941 tggcgcattt ggcatcgacg catgactgcc ggatgcggcg taaacgcctt atccggccta
    42001 cggttcaggc acaggtttgt aggcctgata agacgcgtaa gcgtcgcatc aggcaatggt
    42061 gctcggatgc cggatgcggc gtgaacgcct tatccggcct acgagatgaa gcgatgtttt
    42121 ggccttacgc gacctttttt gcacgctctt cacggcgtgc caaatattca gccactgttt
    42181 caatccgtcc caattcttcc tgggtgcgca tgttgttttc aacttcccaa agatcaactg
    42241 ccgcctggag gtttagccaa aaatcgactg tggtatcaaa aactttcgcc agacggaatg
    42301 ccatctcagt ggtgagttta cgattgttat tgatcagtgc actgacgcta ttacgatgaa
    42361 catgcagcaa ctctgctaac tcattgattt tcaaatctag cggttccaga tattcataga
    42421 gaagaatatc tccgggtgtc gtcggttttc ttgttgcctg tttcatagtt tttaactgtt
    42481 gcttccctgt aaatagcgat acaagaacat actgaatcat acaaacacat taaacaagtc
    42541 cccaactcca tttgcgccat ccctccccgc gcgctagcat gatatttcac aaagggaggt
    42601 cgtatggatc tgttgtaccg ggtaaaaacg ctttgggccg cgctgcgcgg taatcattac
    42661 acctggcctg ccattgatat ctctcttccc ggcaatcgcc attttcatct gattggcagt
    42721 attcatatgg gtagccacga tatggctccc ctacccaccc gtttgctcaa aaagctcaaa
    42781 aacgccgatg cgctgatcgt cgaggcggat gtttccacca gcgatacgcc ttttgctaat
    42841 ttgcctgcct gcgaggcgct ggaagagcgc attagcgagg agcaactcca aaaactgcag
    42901 cacattagcc aggagatggg catttctccc tcactctttt ctacccaacc gctgtggcaa
    42961 atcgcgatgg ttcttcaggc gacgcaggca caaaaactgg ggctgcgggc agaatacggt
    43021 atcgattatc agctattgca ggcggcgaag caacaacata aacccgtgat tgaactggaa
    43081 ggggcggaaa accagattgc catgttgctc cagctccctg acaaaggact ggcgctgctg
    43141 gacgatacgc tgacccactg gcataccaac gcacggttgc tgcaacaaat gatgagctgg
    43201 tggctgaatg cgccgccgca aaataatgaa ataacgctgc ccaatacgtt cagtcagtcg
    43261 ctgtacgatg tgctgatgca tcagcgaaat ctcgcctggc gggataaatt acgcgccatg
    43321 ccgccggggc gatatgtggt cgcggtcggt gcactacacc tgtatggaga agggaattta
    43381 ccgcaaatgt tgcgctaaaa aaatggccaa tatcgctatt ggcccgtcaa agaggaattt
    43441 cattttttta ttattatgcc gtcactttaa gcgacggtgt aactcgattg tcgctcaatc
    43501 tcaccatcct gccaatactc ttaggcaaga tgactctttt ttttggaccg ccatcaggcg
    43561 tatgcttgct tcgttttttt gtttaaggca ggaaaattac catgacaccc gcagttaaat
    43621 cactcgaaaa aaacaagatt tcgtttcaaa tccataccta cgagcacgat ccggctgaaa
    43681 ccaattttgg cgatgaagtc gtcaaaaaat taggtttgaa tccggatcag gtctacaaaa
    43741 cgctgctggt cgcagtgaac ggtgatatga aacaccttgc cgtggccgtt acgccggtcg
    43801 caggtcaact ggaccttaaa aaagtagcaa aagcgctggg tgccaagaaa gttgagatgg
    43861 ccgatccgat ggtcgcgcag cgttcgacgg gatacctggt tggggggatt agcccactgg
    43921 ggcagaaaaa acgtctgcca acgattatcg acgcccccgc acaagaattt gccactattt
    43981 atgtttccgg cggcaagcgc ggactggata tcgaactggc ggcaggcgat ctggcgaaga
    44041 tcctcgatgc caaatttgct gatatcgccc gccgcgatta agcattgtgc cggatgcgaa
    44101 catccggcac tttcggatta ctgccagctc acctcacctt tcggttcata aacactcaca
    44161 tccagcggcg agcttttctg gatatacgct ttcagcactt cggcatcaat aaagccggta
    44221 ttcacatagc ccggtttgtt atcaaggcgc ggatatccat caccgccggt ggcattgaag
    44281 tttaatgtcg ccatacggta agttttcgct ggatcgaccg gttcgccttt gattttaagg
    44341 tcgttcagtt tgccgtcttt cgccacaaag ctaacgttgg caaattgcgg gtaggcacct
    44401 gaatctggct tcatctgcgc gacggcggtc aggtaatcaa tcacctcttt accggtcatg
    44461 tcggcataca ccaccacatt gccgaatggc tgcactttca gcacgttttt atagctgata
    44521 tcgcctgctt cgatagaatc acgaattccg cctccgctca tcaccgcaaa gtcggcacca
    44581 gtgcgatcca tttgtgctgc cagaatcaac cgccccatat tggtctgtac aaaacgcact
    44641 ttgtcacgat cgccttccag acgaccattg gtttcgccta ttttcacttc cagctgcgct
    44701 ttgcctttgt tctggaacgg cgataacagc gagatcattt gctggttttc agcgatttca
    44761 ggggtgtaca gcacgcgctc gcttttcccg tcttcccagg tcactttctt cttcaggttc
    44821 accggaatca gctggtagtt aaccattttc atttcgccgt tgcgaaactc aaagtcggcc
    44881 cgaccaacgt acttgcccca ctcatgcgcc tgtacaatcc agatgccgtt ttgttgatcc
    44941 ggtttgcatg gcgtacctgg aacgtaatct acctgctttt tattttccgc tgccatacag
    45001 actggatctt gcgagtgacc gccgacaatc atcgccagcg atccggcagg caacgcgcgt
    45061 gccatctcca catcgcccgg tgcgttagaa ccgtgatcgc cgttatcgta atgccccata
    45121 tgtgtcgccg cgataataat gtctggcttt tctgtctgtt gcagctcctg aatcaccagc
    45181 ttcgcttcat cggcgggctt acggaactca atatcggtga agttttctgg attgcctaac
    45241 ttcgctgtgt catccgtcgt cagaccaata acggcaattt tcagatcctg acgcttaaac
    45301 agcgcccatg gtttaaacag gcgctcgccg gtacttttct ggtagatatt ggcagaaagt
    45361 aacgggaact ttgcccactt ttcctgctgg cgtaatacgg tgagcgggtt atcaaattca
    45421 tgattaccga tcgccatcgc gtcatagccc accagattca taccgcgaaa atcaggttcg
    45481 gcatcctgta agtcagactc gggcacgcca gtgttaatgt cgccaccgga aagtagcagc
    45541 acgctaccgc cttcagccgc aacctctttg cggataccat ccaccagcgt tttttgcgcc
    45601 gccagaccat attcgccata ttcattgcgc caaaaatgcc catgatgatc attggtatgc
    45661 agaactgtaa ttttgtaggt tttatcctgc tcatacgcca gagcagcttc actcgccagt
    45721 gtaaatgtgg ttaacagtgc taacgccacg ccccgctgca ataatttcat acttctctcc
    45781 ctgacctgat ttcaacgtgt tgaaaaatag tatgacgcga catcatagac aagtctgcgt
    45841 ccggtgaaga taaatctgca tccagattag aaattctgta ttcagcgttg agacttcctt
    45901 caggcggcgc atacaggtat gttagtcggg taataatcac aaataagatc attcttaaca
    45961 tcgcaaccct gaaaaagtga ctttcctatg gcaatgagtg aacaaaccca gcctgtggcg
    46021 ggcgcggctg cgtcaacgac caaggcccga acatcgtttg gtattttagg tgctatcagc
    46081 ctctcacatc tgctgaacga catgatccaa tcgctgattc tggcgattta tcctctgctt
    46141 cagtcagaat tttctctgac atttatgcag attggcatga taaccctcac cttccagctc
    46201 gcctcttcgc tactgcaacc agtggtcggc tactggaccg ataaatatcc gatgccgtgg
    46261 tcgttgccaa ttggcatgtg ctttacctta agtggtctgg tgctgcttgc gctggcgggc
    46321 agttttggcg cagttctgct ggcggcggcg ctggtcggta ccggttcatc ggtctttcat
    46381 ccggaatctt ctcgcgtggc ccgtatggct tccggcgggc ggcatggcct ggcacaatct
    46441 atctttcagg tcggcggcaa ctttggcagt tccctgggac ccttgctggc ggcggtgatt
    46501 atcgcgcctt atggcaaagg caacgttgcc tggtttgtgc ttgcggcact gctggcgatc
    46561 gtggtgttgg cgcaaatcag ccgttggtac tcggcacagc accgaatgaa taaaggaaaa
    46621 cccaaagcga cgattatcaa tccactgccg cgcaacaaag tggtactggc ggtcagcatt
    46681 ctgttaatcc tcattttctc gaaatatttc tatatggcga gcatcagcag ctattacacc
    46741 ttttatctga tgcaaaaatt cggattatct atccagaatg ctcagcttca tctgtttgcc
    46801 ttcctgtttg ccgttgcggc aggtacggtg atcggcgggc ctgtagggga taaaattggg
    46861 cggaaatatg tgatttgggg ctctatcctc ggcgttgcgc cgtttacgct gattttaccc
    46921 tacgccagcc tgcactggac gggggtttta acggtgatta ttggatttat cctcgcttcg
    46981 gcattctctg ccattctggt ctacgctcag gagctgcttc caggacgtat cggtatggtt
    47041 tctggactct ttttcggttt tgctttcggc atgggaggtc tgggagcggc agttctgggg
    47101 cttatcgccg atcacaccag catcgagtta gtctataaaa tatgtgcttt cctgccacta
    47161 ttggggatgt tgaccatatt cctgcctgat aaccggcata aagactgatt tcattgcagc
    47221 caaaggcaaa ctttggctgc atcgttttac agtcgccata agcctttcct ctgttaaacc
    47281 gccttctgtt ttccgatccg tgcttttttg cagctaattc catttttctg caaaattcaa
    47341 taattattca tgaacatcat caatgaaatt gtcataaact attacctgta attttggttt
    47401 tcccggccaa aaatggacaa taaccaccag gaaaaggaga cggaatgcat cacgccaccc
    47461 cgcttatcac caccattgtt ggcggccttg tgctcgcctt tatcctcggc atgctggcca
    47521 ataaactacg tatttctcct ctggtgggat atctgttagc gggtgtgctg gcaggaccat
    47581 tcactccggg ctttgttgcc gataccaagc ttgcgccgga actggctgaa ctgggcgtca
    47641 ttctgttgat gtttggcgtg ggtctgcact tttcgctcaa ggatttgatg gcggtaaagg
    47701 ccatcgccat tcccggtgcg atcgcccaga tagccgtggc gacgctgctg ggtatggcgc
    47761 tctctgccgt gctgggctgg tcgttaatga ccggtatcgt gttcggttta tgtctttcca
    47821 ccgccagtac cgtggtgtta ctgcgcgcac ttgaagaacg gcaattaatt gacagtcagc
    47881 gtgggcaaat cgccatcggt tggttgattg tggaagacct ggtaatggtt ctgacgctgg
    47941 tgttgctgcc cgcagtggca ggaatgatgg aacagggcga tgtgggcttt gccactcttg
    48001 cagtcgatat ggggatcacc atcggcaaag tgatcgcgtt tatcgccatt atgatgctgg
    48061 taggtcgccg tctggtgccg tggattatgg cacgcagcgc ggcaaccggt tctcgcgagc
    48121 tgtttaccct gtcggtgctg gcgctggcgt tagggattgc ctttggtgcg gtagagctgt
    48181 ttgatgtctc ctttgcactc ggtgcgttct ttgccgggat ggtactgaac gagtctgaac
    48241 tgagtcaccg tgccgcccac gatacgctgc cattgcgcga cgcgtttgcg gtgctgtttt
    48301 ttgtctccgt cgggatgttg tttgatccgt taattctgat tcagcaaccg ctggcagtgc
    48361 tggcgacgct ggcgattatt ctgtttggta agtcgttagc cgcgtttttc ctggtgcgac
    48421 tgtttggtca ctcccaacgt acggcattaa ctatcgccgc cagcctggcg cagattggtg
    48481 agttcgcgtt tatcctggcg ggactgggaa tggcattgaa tttactgccg caggccggac
    48541 aaaacctggt actggcaggg gcgatcctgt cgattatgct caacccagta ctgttcgcac
    48601 tactggagaa atatctggcg aagaccgaaa cgctggaaga gcagacgctg gaagaggcaa
    48661 tcgaagaaga gaagcagatc ccagtggata tttgcaacca tgcgctattg gtgggttacg
    48721 gtcgtgtagg cagcctgctg ggggagaaat tgctcgcctc tgatattccg ctggtggtga
    48781 ttgagacgtc acgaacccgt gttgatgagc tgcgagagcg cggggtccgc gcagtattgg
    48841 gcaatgcggc gaacgaagaa attatgcaac tggcgcatct ggaatgtgca aaatggctga
    48901 tcctgacgat ccccaacggt tatgaagcgg gtgagattgt ggcatctgcc cgcgcgaaaa
    48961 atccggatat tgagattatt gcccgcgccc attatgacga tgaagtggcg tatatcaccg
    49021 aacgtggtgc gaatcaagta gtgatgggcg agcgtgaaat tgcccgtact atgctggaac
    49081 tgctggaaac gccaccggcg ggtgaagtgg tgacggggta aacggtggtt gccggatgta
    49141 aaatatgaaa aatatgtagg catgataaga cgcgccagcg tcgcatcagg cattgagagc
    49201 aaaatgccag atgcggcgtg aacgccttat ctggcctacg aaccgacgat aacttaacga
    49261 tcccagtaag actcttccag gctgtcttca cgctccggca agccgcgcgt taaacgaggt
    49321 gaatgctggt tcagtacctg atagctcaca cggttagcat atttacacac ctgcgctaac
    49381 gatgaataag ttaaccaggt gaatttatgt ttgctggagt ttggtacgtt gctacgatgg
    49441 tagctgttgg cggtaatgtc atgcagcaac gctgccagtg cgccatcccc tgctccgttg
    49501 gtgttcatga ttttttccgg cccgcccatg tacggcgcaa tgtgcgaata gacacgcagc
    49561 ggattctgac aatccttgtg gcgcatggcg cggctaaact catactggtt gaattcggct
    49621 atagcgcccg gcagcaacgg atgctgggtt ttacgtttcg cttcgtcttc ggtaaagccc
    49681 gccatataca agccgattgg cccggcggtg cacagcacca gatctaccca gtccagcgcc
    49741 ttgtcagatg ccaacaacgg atcgctttct ccggtcaacg cttcggcttc atcttcgttc
    49801 atcgcaagga tagagacgtg gtctttgagg aattgctgcc accactgtgg attctcggca
    49861 atgacaaact tagttcccag cgtcagcacc accggtacgt tatatttctt cgcgtactca
    49921 atggctttca tggttgcttc cggcatgggt tcacccggct tgcaacgcac cagataagag
    49981 gtgagaacca gagccgaggc tccggcaatc acatcttccg gaatactttc agcccgcagc
    50041 tggttcatgt ggccagggct gatagcaaag gtacgttccc cggactcgcc aatcagcgta
    50101 aagcaacgac caatcgggcc atccacgcct tgtagatagt taagatcggt acggctggag
    50161 gtgttacaca ggtaacgata ggcatagctg ccaatttcaa tattgctgca catgacgccc
    50221 agcagcaccg aacggtcgtc cgcgagcacc gagtagttat gcatggtgtt accaatggtg
    50281 ccacccgcaa actgatgggt aatcaggttt ttctgtttta gttcctgata aagcgcttcg
    50341 gctacatcat cctcaatcac cagtgaatgc ccggcgctta atccataacg ctcaatgaat
    50401 tcatcatcca ctttcgcttc aatatcgacc agcgtttgat cgatacccac tacccaggcg
    50461 gcgctggttt cgttttctgg ctggaattgc tgaagcagcg gatcgcgtgc atttacgggg
    50521 aagtaatgtt tggatttacg tttaccggga aatttcatgg ttgtttttac gggtggttaa
    50581 ctgagcggcg aatgttaaca caatctgacg ggagcgcgcg agacgcagcc agtttcaata
    50641 tcagcaggcg ggatcgcatt ttcatggcag tggttcctta caatgacgta atttgaaagg
    50701 agtttttgta tgaagccgga aaacaaatta cctgttctgg accttatttc tgctgaaatg
    50761 aagaccgttg tgaatactct tcagccggat ttaccgccct ggcccgcaac gggaacgatt
    50821 gctgagcaac gacagtatta cacgcttgag cgccgattct ggaatgcggg cgctccagaa
    50881 atggcaacca aagcttacat ggttccaaca aaatatgggc aggtggaaac acgtctcttt
    50941 tgtccgcagc cagatagccc ggcaacgcta ttttatttgc atggaggcgg ttttattctc
    51001 ggcaatctcg atacccacga tcgcatcatg cgcctgctgg caagctacag ccaatgtacg
    51061 gtgattggta ttgattacac cctttcacct gaagcgcgtt ttccgcaagt gatagaggaa
    51121 attgtggctg cttgttgtta tttccaccag caggcggagg attatcaaat caatatgtcc
    51181 cgcattggct ttgccggtga ttccgcaggt gccatgctgg cgctcgccag tgcgttgtgg
    51241 ttgcgtgata aacagatcga ttgcggtaaa gttgcgggcg ttttgctgtg gtatgggctt
    51301 tacggattac gggattccgt gactcgtcgt ctgttgggcg gtgtctggga tggcttaacg
    51361 caacaggatt tgcagatgta tgaagaggcg tatttaagca acgacgcgga ccgcgagtcg
    51421 ccgtattact gtctgtttaa taatgatctc actcgcgaag ttccgccctg ttttattgcc
    51481 ggggcggagt tcgatccgct gctggatgac agccgtctgc tttaccagac gttagcggcg
    51541 catcagcagc cctgtgagtt caaactctac ccaggcacgc tgcacgcctt tttgcattat
    51601 tcacggatga tgaaaaccgc cgacgaggct cttcgcgacg gcgctcagtt ctttaccgct
    51661 cagctttagc gatacgcggc aacaagatta gccatcattt cgatatgttc cggtgtggca
    51721 ttaagcgccg gaatatattc atattttttc ccgccggcac cgaggaagac ctcacggttt
    51781 tgctcggcaa tctcttccag cgtctccaga caatccgcag caaagcccgg gcacatcacc
    51841 tgaatatgac ctacgccttt ttctccgagc attttcagcg tttcgtcggt ataaggcatc
    51901 agccagggtt cccgaccaaa gcgcgactga aaggtcatca tcactttttc cggtgccatc
    51961 cctaatgcgg aagccagttc acgagtcgtt gtgcggcaac gttgcgggta atcatcgcct
    52021 tcatctgcat aacgctgggg aatgccatga taagagagca gcagcagatc cggttcgcca
    52081 tgtttggcaa aagaagcgcg tacgctgttc gccagtgcat taatgtaatc gtggttatcg
    52141 gcgtaatcac gtataaacga tatccccgga atgctacgtt tgcgcgccag aatgcgtgcc
    52201 agttcatccc ataccgcacc gaccgtagaa caggagaatt gcggataaag cggcagcacc
    52261 acaatatgat ctacatgctc tgccaggagt tcatctacgg cgctttccag tgatggcgag
    52321 ccgtagctca ttcccagcgc tacgggcgtc tccggtaaac gttgtgccag cgcctgctgt
    52381 tgctggcggc tgtaaaccat cagcggcgag ccaccttcca tccagacaga ggcatacagc
    52441 ttcgccacac gcggcgagcg cagcggcaaa atcacgccgc gcagcaatgg ccaccataac
    52501 aaccgtgagg tatcaaccac gcgtctgtcg cttaaaaatt gtttcagata gcgttttacc
    52561 gcttcaggtg tgggggcatc gggcgtaccc aggtttgcca gcaggatacc ggttttagtc
    52621 tgacgcatta ccgcctctta tcgattcaac ttgttgataa ttgtagcgga aaagtgagga
    52681 agaagaacta attgctgtga gaggtattgc ctgaggtgga tgagcagacc tgcccgaaag
    52741 cagatctgct gcggcttgat tagccgagga ttttttccag atcagctcga acttcagcaa
    52801 ccggcttggt gccgtcaact ttcgcgtatt tggtgttacc cgcttccgct tctttggagt
    52861 agtagccgat cagcggtgct gtcatctgat ggtattcaac cagacgttta cgtacggttt
    52921 cttcctgatc gtctttacgg gtagtcagtt cttcaccggt aacgtcgtct ttaccttcta
    52981 ctttcggcgg attgaattta acgtgataaa cacgaccaga cggcgcgtga acgcggcgac
    53041 cgacgatacg gtcaacgatc agttcgtccg gtacgtcgaa ttccagaacg taatcaacat
    53101 tgatgcccgc ttctttcatc gcgtctgcct gcggaatggt acgcgggaag ccgtccaaca
    53161 ggaaaccatt acggcagtct tcctgagcaa tgcgctcttt aaccagcgcg atcaccagtt
    53221 cgtcggtgac cagtttgcca gcatccataa tgtcttttgc ttgtttaccc agctcggagc
    53281 cagatttgac cgcagcacgc agcatatcgc cagtggagat ttgcggaata ccatatttct
    53341 ccatgatgaa ctgagcctga gtccctttcc ccgcgcccgg agcgccaagc agaatgatac
    53401 gcattgcgaa aatcccctta aaatgtgtcg aattttttga aaaagcgata aacgatacca
    53461 ccatcaggcg attgcctcaa gaaaggcacc aggggctgaa acggttaatg gcgagataat
    53521 ttgcggaaag tgcagataat gaaaagaaaa atgccggatg acacgaaggt catccggcat
    53581 tacatcagga aaccagcagc tggttcatac gacgaataaa caggttcgga tcttccagcg
    53641 tgccgcgttc tgccagcagt gcctgatcca gcagcagttc tacccactcg ctgaacttcg
    53701 cttcatcttc agtatctgcc gcacgtttca ccagtacgtg atccgggttc agttcgaaga
    53761 tgtatttcac ttctggcact ttctggcccg ccgcagcgaa cagtttcgcc atctgagtgc
    53821 tcatttcgtc cgcgtcggtc gaaacgatcg ctggcgtatc ggtcagacgg tgagtcagac
    53881 ggacatcttt cacgcgctcg ccgagcaggg ctttcacacg gtcgatgaac ggagtcagtg
    53941 ctttctccgc ttctttcgcg ctctcatcaa cttcgtcagc cagtttttcc agcgactcgt
    54001 caactttaga caccgactgg aacggtttac cgtcgaactc agtcagatag ttcatcatcc
    54061 actcatcgat gcggtcggaa agcagcagaa cttcgatgcc tttcttacgc agcagttcca
    54121 ggtgcgggct gctcttcgct gccgcatagc tgtctgcagt gatgtagtag attttctcct
    54181 gcccttcttt catgcgggaa acgtagtctt ccagagacac ggtctgcgca gaagaatcgg
    54241 tatgggtaga agcaaaacgc agcaatttgg cgatcgcttc ctggttagcg aaatcttccg
    54301 ccggaccttc tttcagtacc aggccaaact gttgccagaa ggtctggtat ttttccgcgt
    54361 cgtctttcgc cagtttttcc agcatttgca gcacacgctt ggtcagcgca ttgcgcaggt
    54421 tacgcgttac cgtgctgtcc tggaggattt cacgggaaac gttcagcggc agatcgctgg
    54481 agtcaatcag accacgcacg aagcgcagat agttcggcat gaactgttct gcgtcgtcca
    54541 tgatgaacac acgctgaaca tacagtttca ggccgtgttt atgatcgcgg ttccacatat
    54601 cccacggagc ctgggacgga atgtacagca ggctggtgta ctcctgctta ccttcaacac
    54661 ggttgtggct ccaggtcagc ggatcgttaa aatcgtgggc gatgtgtttg tagaactctt
    54721 tgtactcttc atcggtgatt tccgacttgt tacgagtcca cagcgcctgc gctttgttaa
    54781 ttttctccca ggagataacg gtttcgccgt ctttctcttc gcgtttttcg atctctaccg
    54841 gcagcgcgat atggtcggag tatttgctga tgatggaacg cacgcgccag tcatcgagga
    54901 actcgtcttc gccttcacgc agatgcaggg tgatttcagt accacgatct tctttggtga
    54961 tgtcggcaac ggtatattca ccttcgccag ccgattccca gaagacgccg ttttctggtt
    55021 tttcgcctgc cgcgcgagta cgcacggtca ctttgtcggc cacgataaac gcagagtaga
    55081 aaccaacacc aaactgaccg atcagctggc tgtctttcgc ctggtcagaa cccagggatt
    55141 cgaggaatga tttggtaccg gatttagcga tagtccccag atggtcaatc acttcgtcgc
    55201 gggtcatccc cacgccgtta tcggagatgg tcagcgtgcg cttgtcttta tcgaaagaga
    55261 cacgaacgcg cagttcgcca tcaccttcgt acaggtccgg gttagagagc gcacggaaac
    55321 gcaacttgtc cgccgcatcg gaggcgttag agataagctc acgcaggaag atttctttat
    55381 tggaatagag agaatggatc atcaggtgca gaagctgttt cacttctgac tgaaaaccac
    55441 gagtttcttg tcctttcatg taggtctacc tcaataatgc cattttaagg ttaaaaacag
    55501 gatgagggag agatggggac aagggagaat aatttcaagc gagagcaggt gatcctgctc
    55561 tcgtttgctt aaaaacgaat cttatgacgc ccggcaaggg agtgtgacag cgtggtgcca
    55621 tcgaccattt ccagttcgcc gccaaccggt acgccatgag cgattcggct ggcttccacg
    55681 tcatattgcg cgcaaagctc ggcaatgtag ttagcggtag cttcaccttc aaccgtaggg
    55741 ttggtggcga ggatcacttc agtgattttt tcctctgcca gacgctgttc cagacgatca
    55801 aggccgatat catccggacc gatgccgtcc agcggtgaca gatgtcccat caacacaaaa
    55861 taacgacctg aaaactgccc cgtctgctca atggcgtaaa tgtccgccgg actctccacc
    55921 acgcagattt gaccgttttc ctgacgacgc ggattcgaac agatgttaca gacttcctgt
    55981 tcggtgaagg tgcggcaatc ggcgcagtgg ccgatttccg acatcgcccg ggtgagcgcc
    56041 tgcgccagac gcatcccgcc gctacgatcg cgctgaagca gcgtgaacgc catacgctgc
    56101 gccgacttcg ggccaacgcc cggcagacag cgcagtgctt ccataagctg tgttaacagc
    56161 gggctggttt gcatcagaac ggcatcttaa agccaggcgg cagctgcatt ccggaggata
    56221 cagaagccat tttttctttc tgcgtttctt caatacgacg tgctgcgtcg ttgaatgctg
    56281 cagccaccag gtcttccagc atctctttgt cgtcttccag caggctcggg tcgatctcta
    56341 cgcgacggca gttatgtgca ccgttgatgg tcacttttac cagacctgcg ccagattcgc
    56401 cggtaacttc cagctgcgcg atctcttcct gcattttctg cattttttct tgcatctgct
    56461 gggcttgctt catcaggtta cccagaccgc ctttaccaaa cataggtttc tctctcaatc
    56521 acgttaagga tgacgaacgt aagctgtgct tacgatcaaa tggggcggat actgtcttca
    56581 tccagctccg catcgaagaa ccgacgcagg gtctgaatat tattatccgc aataatggac
    56641 tcgcgcgcct gcgcaagttt ttcttcgtat atcgcctgac gccactccag cggcgtacgc
    56701 accgcgggat tatcatcttc aactatagtc agttcaaccg ttgaaccttt taacatgctc
    56761 aacgcttcag ccagtttttg ctgtgcaccg cggttgttca aatgccgctg agaggagcgc
    56821 aaatgcagac atactgcgtt gtcgctctcc tctttccagg catttaacgc cacctgttcg
    56881 accagttttg gtagcgaaag ttgactcacc tgagccgccc acgcgtcgcg ctcaatggct
    56941 tctgccgcca gcttcgccgc cagttccggc gttttttcat gttccagcgc ttttttcagc
    57001 gccttcggtg tggcgaccac ttctttttgc tgcatcactg gagtggtcgc cttccagcga
    57061 tacgcttctt ttttagctgg cgctttttcc agcgccgatg gcaccggacg cgcctgaacg
    57121 cgatcggtga ccgaagccag tctttccagc gcagcgttat tcaccggccg cgcgcgggta
    57181 gcggctgccg gttcactctt ttttgctttg gttgctccct gcacgcgctg caactgctgg
    57241 cgcgccgcca gcacctggct ggtggtttcc gggagcggta cagtcggtgc ctgctgcggc
    57301 gctgattgcg gttgcggcgg cacctgggtt ggcgtcatta ctgccgttgg cgcgacgggt
    57361 gcaaaggact ggcgtggcac ttctggctca ggcagcggca tacgcggatg gaacgccagc
    57421 gcgcgcagca gcgtcatctc aacgcccatg cggcggtccg gcgcatacgg taattctttg
    57481 cgaccaatca acagcgtctg atagtaaagc tgaatatccg tcggcggtat ggtgcgcgcc
    57541 agttcacgca tccgcagctc gatggcggcc atgtcgttgc caagtgcagc aggcgaaagt
    57601 tgtaccatcg caatacgatg caacaggccg agcatttcca ccagcaacgc ttcccactcg
    57661 ataccacggg cagcggcttc attaatcagc gccattacgc gctcgccgtt ggcctcgacc
    57721 atcgcttcaa ccagcgacag cgcctgatcg tcgtcaagcg tacccagcat cgcactgacc
    57781 gcctgggttg aaacctggcc gtcaccgctg gcaatcgcct ggtcggtcag acttaaggca
    57841 tctcgcaggc tgccttcagc ggcgcgcgcc agcaattgca gcgcccgcgg ctcgtgagcg
    57901 atatgttctt cattgaggat gtgctcaagt tgatgacgaa tttgctcgac atccagcgcc
    57961 ttgagatgaa attgcaaaca gcgcgacaaa atcgtcaccg gcaatttctg tggatcggtc
    58021 gtcgccagca gaaacttaac gtgctccggc ggctcttcaa gggtttttaa cagtgcgtta
    58081 aagctgtggc gcgacagcat atgcacttcg tcgatgagat aaactttgaa acgaccacgc
    58141 gccggagcgt actggacgtt atccagcagg tcgcgggtat cttcaacttt ggtgcgcgag
    58201 gcggcgtcga tttcaatcag atcgacaaag cgcccctgct cgatttcacg acagttatcg
    58261 cacacgccgc acggcgtcgc ggtaatgccg gtttcgcagt ttagcccctt cgccagcagt
    58321 cgggcgatag aggtttttcc gacgccacgg gtgccggaaa aaagataagc atgatgaata
    58381 cgccctaacg acaagccgtt cgccagtgcg gtcagcacat gttcctggcc gacgacgtca
    58441 gcaaaggttt gtgggcgcca ttttcgggct aagacctgat aactcattgg caggctctga
    58501 aacgctggaa ggtggattct cgaaggggta atgctaacac agccccgtca gaacggcgag
    58561 gctgaatgca catcttgtgc tgtccgtagc gtgggcagca caagactggc gataattaat
    58621 ggcccgggaa cgggacaagg ctgtagctgg taatgccctg tttttcgaga cgctgttcgc
    58681 cgccgagatc gaacaggttg ataatgaacg cagcgtcagc cacttcacca cccagacgac
    58741 ggatcagttt aacggtcgct tcgatagtgc cgccggttgc cagcagatcg tccaccacca
    58801 gaactttgtc gcccggtttg atggcatcaa cgtggatctc cagctgatcg gtgccgtatt
    58861 ccagatcgta agtttcactg atggtttcac gcggcagttt gcccggttta cggaccggta
    58921 caaagccaac gcccagaccc agagctaccg gagcgccaaa caagaagcca cgcgcttcgg
    58981 tgccgacaac tttgttaatg cccgcatttt tgtaacgctc aaccagcaag tcgatgctga
    59041 gagcgtaagc tttcgggtct tccagtaagc tggtgacatc gcggaaaaga atgccgggtt
    59101 ttgggtagtc ctgaatgctt ttgatgctat ttttgagata ctcaagctgc tgtgcagtcg
    59161 cggtcataag tgtatgcctg cttgttacgg tggtactcac ggcgcgtttt aaacgtatca
    59221 aaagtaacag ttgtttaacc tttgaccgcc tgcgcctgtg ctcgaaaacg ccagaatgta
    59281 ctggctgtac gcaataattg caactgcgat tgttgtgctt cagtgctttt cttgcttttc
    59341 atcaatcacc ggaattcgcc acatatagaa aagcaaacag gcgagaatta ccagcaacat
    59401 gatgcgcacc catggcatct ggacgaacca cagagaaatg gcaaacgtga gcaaaataag
    59461 caaaatcgcc cgcggtttga cgccgcgcgg catcgcatga tgtttctgcc agaaacgtag
    59521 atagctgcca aaccatgagc ggtacagcaa ccaggcgtga aagcgcgggg aagaacgggc
    59581 aaagcaccag gccgccagca ggataaacgg cgtcgtcggt aataccggta ataccacgcc
    59641 cagcgtgccc agcactaccg ccagccagcc aatgatgatt aaaatgattc gttgcatatt
    59701 gagtgttgtc agcttacaga gtggctactt tagcataaca attatcattt tcattgaggt
    59761 cttatcgtga aaaccgccct gctgctggaa aaactggaag gacagctcgc tacgctgcgt
    59821 cagcgttgtg ccccggtgtc acagttcgcc acgctaagtg ctcgtttcaa caggcatctt
    59881 tttcagactc gtgcgacaac actacaggct tgtctcgaca aggcgggcga taatctggct
    59941 gcgcttcgtc atgcagttga gcaacagcaa ctgccgcaag tcgcctggct ggcagaacat
    60001 ctggcggcgc aactggaggc tatagcgcgt gaagccacag cctggtcgct acgcgagtgg
    60061 gatagcgcac caccccaaat tgcccgctgg cagcgtaaac gtattcagca tcaggatttt
    60121 gagcggcggc tacgtgagat ggttgccgaa cgtagagccc gcctggcgcg ggtgaccgat
    60181 ctcgtggaac agcaaacgct gcatcgtgaa gtggaagcct atgaagcgcg cctggcacgc
    60241 tgccgccatg cgctggaaaa aatcgaaaac aggttagcgc gtttaacccg ctagcaatgg
    60301 agagagtatg tcactggaaa atgcccctga cgatgtcaaa ctggctgtcg atttgattgt
    60361 gctgctggaa gaaaatcaga tcccagcccg caccgtgctg cgcgcgctgg atattgtaaa
    60421 gcgtgattat gaaaagaagt tacagagcga tgaggcttca caaagcgagt aaaaaataaa
    60481 gggcggcatc actgtcgccc tttattgtcc ttaccctacc gctggcgtcg gatcgtcgcc
    60541 tttgtagtcg cgttttactt ccgtcacctc atcgcccttc tcgttgtgca gatgcacttc
    60601 aagctggtta aaggcaatgt tgatgtcgtt ttcacggcac agctgatcga tagtacggtt
    60661 cagctcatcg acagtacgac tacggtcacg cagttcacgc acatacagac gcagctcatg
    60721 atccaacgtg ctggcaccaa atgccgtaaa gaagacttcc ggcattggtt cgtgcatcac
    60781 ccttgggtgc tcagtcgccg ccttcagtaa cactttacgc actttttcca gatcggagcc
    60841 ataggccacg ccgagacgga tcaccagacg cgtagtagtg tcagtcaacg accagttgat
    60901 cagacgctcg gtaacaaacg ctttgttcgg gatgatcact tctttgcgat cgaaatcggt
    60961 aatcgttgtc gcacgaatac ggattttact taccgtcccc gagaagctac caatggttac
    61021 cgtatcgcca atacgcaccg gacgttcgaa cagaatgatc aaaccggaga cgaagttacc
    61081 gaaaatttct tgtaaaccaa aaccaagacc tacggataat gctgcggcca gccactggag
    61141 tttatcccaa gagacgccca gcgatccgaa caccgtcatc gcaccaacag caataatgat
    61201 gtagttaagg atggtagtaa tggcatacga cgcgccctgg cgcatattca gtcgcgagag
    61261 caccagcact tccagcaaac caggcaggtt gcgaatcaac gcccaggcca ccattgaggc
    61321 gataatcgca aacaacagac tacccatggt gacgtttttc accaccgcag caccagcttc
    61381 agtaccgttg taatgccaca gcgtaatgct gtcgagatag ctgaacacgg tgatcaaatc
    61441 ggaccaaatt gcccagaaca tgacaccgaa cagcgcaaac atcagcaaca tggtaatacg
    61501 cagcgtctgc tggttaacct gttccagtgc aatggtgggt tcttccggcg gttcggcacc
    61561 ttctgcgccc tctttcacca gattctgccg acgcgccagc gcacgacgcc aggcgatacg
    61621 ccgcgccgct acgcttaagc cgcgcaatac cgtctggtag agcaagttcc agatgatcac
    61681 cagataaacg gtttcaatcc aacgcccgga gaggcgcagc gtggtgtaga aataacccgt
    61741 ggcagtcagc accatcaatg caatcgggat aatcgacagc acggtaatgg tgaccagtcg
    61801 catggtgtgc gactctttat cacgccagct ttcgcggcac attggccata ccaggaaggc
    61861 aatcagcagc aggttgaaga aaatcatcgc ctgccccagc acatcatcca tcagatgcag
    61921 cggggaaagt tctgccacca cagaccagaa atggataggc agcaacgcga gactgatgcg
    61981 gacaatttgc cgacgccagt ggctggtctg ctgttccggc atgccgaagt gacgcacggc
    62041 aacgccgttc ttctccagca ctttccagca caggccaaac accagccaga aaatcgccag
    62101 ttttttgctg aacgaccata gcagttcgct gatgttgagc tgcatggtca acagaatcag
    62161 gccaaccgcg agaataatca ggcacaccgg cagtgcacgg atcaggtcga taaggatcgc
    62221 ttttggtgtg ttgagctggc tgtcgttacg cagggaaccc acagcggaag ccagtttttg
    62281 ttgatacgct ttcagccacc ccagacgcca gtggatcagc ccggcaatca acagcagcgg
    62341 caaaccagcg aggaaagcga taaaaacggc gggccaggct ttttcccagt taaccgtaat
    62401 tttcatcgac ttaaattcat ctttcaggct ttgcgggaac gctttgatcc agtcccagtc
    62461 cattggacgg ttactgttca cccaaaagat ttgctgagtc aggatggatt tcaggttttt
    62521 cgacacactc attaactgct gctggttgat ttgcaggtta atggccatca tcagctggtt
    62581 acccaactgt ttgttgagtt gatccagcaa ttcgcgacgc atatcaacca cttgcaataa
    62641 cgcatcgtga acttcgctgt tgacttcgtt ggtgtgacct tcttccagtt tgttgacgaa
    62701 cgcatcgctc tggaagagtg catcacgctg ctggttaact tcaaactgtt cgagacgcaa
    62761 atccgcgatg cggttggtca tgttttccag ttcatccgcc gagggcagcg tttgttgttg
    62821 ctggtaaagg atacgagaca acagcaggct gcccttcagg acggcaatct gctctttaat
    62881 attgcgttcc gattgcagcg cccgctccag ccagtttttg actttaatgt tttgctgcat
    62941 caactgatta ccgttttcag tcgcggtaat cagacgctga cttaactgct ggttaatttc
    63001 cagttcctgc ttcaccagcg gattagcctg aatacgcgcg gcttcatccg gggagacggc
    63061 ttcctgcgcc gttttttcgg ttaaagtcag gcgcttgctg tttaccgctt cttgcaacag
    63121 ctgcaactgg tgctccagac gggcgctgtt cgccgtcacg taatcacgtt gcttttgcaa
    63181 ggtatcctgc aagacggtat tcccttccag gcttttacgc tgctggtcaa tctccgcatt
    63241 cagcaacgcc tgctgagcct gcattaacac tttctggctg ggacgtaagg ccgtctcgcc
    63301 gacatcagtc ccatccagac gactgcgaat ttgttgcagc tgctgcgaag cgttatacat
    63361 cgcattttgc acgcgttcgg gctgcgtctg taacgaaacc agctggctgt tataagacgc
    63421 cagatcgttt tgtgcgtttt gcaaatcgtc cagcgcctgg gcaacgcgag tttccagctg
    63481 gcgcaacgaa agcgtgctca gaattttgcg cgtttcttcg tcgttatcga catcgctaag
    63541 tgctgttaac gccgcggtcg cctggcgcat tttttccggc gcttcagcga ctttttgccg
    63601 tagctgaacg gtctcttctt ttacgcgatc gattttatcg agggtggcta atgtatctgt
    63661 cagatcctgc tgcaccagtt tgtcctgagc agaaagatct ttttgtttat ttagtgagtc
    63721 aagttgcgcc tgcaggtccg cttttgtcgg cagatcacca ttcgatgacg cccgcgcaaa
    63781 cgccgtgttc tggcataaca agacaaaaac aaaagcaata aatgctgaaa aaacaaaatg
    63841 ccgtgatcgt ttgtaatact ggaacatagt catgatgaat gaaggtttct gaacctgaag
    63901 aacgacctga aaaagtcaaa ccgcaagaat atcacgacgc agtgaaccag aatagcaacg
    63961 acgaaaatgt ccaggaaaaa tcctggagtc agattcaggg ttattcgtta gtggcaggat
    64021 tacgaagcgt ggggcacagg agatacatct ccagtaagat ggcgacgtaa tcgcgggctt
    64081 cttttttaag atcaaaagat tgcggggcaa agagccagtt ttccatcagg ccggaaatat
    64141 agccgcgcat aataattgct gcccgacgcg tcattaaatc cgcaggcaac attttcgctt
    64201 caatacaatg ttttaacgtt tgttctatac ggtcataact ttccaaacag agattacgtt
    64261 gtgcctgttg cacaacagcc atttctccga caaattcgca tttgtggaat ataatctcca
    64321 tcaataatcg acgccgttct tctgtcaccg tggattcaag aacatgaatt aatatctctc
    64381 ttaatactga gagtggatcg ccagggaatt ttgcctgata ctcaagctct agttcaccaa
    64441 tattggattc tgatagttcc cagatctcac tgaacaaatc cgacttgtct ttaaaatgcc
    64501 agtagattgc accgcgcgta acgccagctg cttttgcaat ctcacccagc gaggtggatg
    64561 ataccccctg ctgtgagaaa agacgtagag ccacatcgag gatgtgttgg cgcgtttctt
    64621 gcgcttcttg tttggttttt cgtgccatat gttcgtgaat ttacaggcgt tagatttaca
    64681 tacatttgtg aatgtatgta ccatagcacg acgataatat aaacgcagca atgggtttat
    64741 taacttttga ccattgacca atttgaaatc ggacactcga ggtttacata tgaacaaaaa
    64801 cagagggttt acgcctctgg cggtcgttct gatgctctca ggcagcttag ccctaacagg
    64861 atgtgacgac aaacaggccc aacaaggtgg ccagcagatg cccgccgttg gcgtagtaac
    64921 agtcaaaact gaacctctgc agatcacaac cgagcttccg ggtcgcacca gtgcctaccg
    64981 gatcgcagaa gttcgtcctc aagttagcgg gattatcctg aagcgtaatt tcaaagaagg
    65041 tagcgacatc gaagcaggtg tctctctcta tcagattgat cctgcgacct atcaggcgac
    65101 atacgacagt gcgaaaggtg atctggcgaa agcccaggct gcagccaata tcgcgcaatt
    65161 gacggtgaat cgttatcaga aactgctcgg tactcagtac atcagtaagc aagagtacga
    65221 tcaggctctg gctgatgcgc aacaggcgaa tgctgcggta actgcggcga aagctgccgt
    65281 tgaaactgcg cggatcaatc tggcttacac caaagtcacc tctccgatta gcggtcgcat
    65341 tggtaagtcg aacgtgacgg aaggcgcatt ggtacagaac ggtcaggcga ctgcgctggc
    65401 aaccgtgcag caacttgatc cgatctacgt tgatgtgacc cagtccagca acgacttcct
    65461 gcgcctgaaa caggaactgg cgaatggcac gctgaaacaa gagaacggca aagccaaagt
    65521 gtcgctgatc accagtgacg gcattaagtt cccgcaggac ggtacgctgg aattctctga
    65581 cgttaccgtt gatcagacca ctgggtctat caccctacgc gctatcttcc cgaacccgga
    65641 tcacactctg ctgccgggta tgttcgtgcg cgcacgtctg gaagaagggc ttaatccaaa
    65701 cgctatttta gtcccgcaac agggcgtaac ccgtacgccg cgtggcgatg ccaccgtact
    65761 ggtggttggc gcggatgaca aagtggaaac ccgtccgatc gttgcaagcc aggctattgg
    65821 cgataagtgg ctggtgacag aaggtctgaa agcaggcgat cgcgtagtaa taagtgggct
    65881 gcagaaagtg cgtcctggtg tccaggtaaa agcacaagaa gttaccgctg ataataacca
    65941 gcaagccgca agcggtgctc agcctgaaca gtccaagtct taacttaaac aggagccgtt
    66001 aagacatgcc taatttcttt atcgatcgcc cgatttttgc gtgggtgatc gccattatca
    66061 tcatgttggc aggggggctg gcgatcctca aactgccggt ggcgcaatat cctacgattg
    66121 caccgccggc agtaacgatc tccgcctcct accccggcgc tgatgcgaaa acagtgcagg
    66181 acacggtgac acaggttatc gaacagaata tgaacggtat cgataacctg atgtacatgt
    66241 cctctaacag tgactccacg ggtaccgtac agatcaccct gacctttgag tctggtactg
    66301 atgcggatat cgcgcaggtt caggtgcaga acaaactgca gctggcgatg ccgttgctgc
    66361 cgcaagaagt tcagcagcaa ggggtgagca ttgagaaatc atccagcagc ttcctgatgg
    66421 ttgtcggcgt tatcaacacc gatggcacca tgacgcagga ggatatctcc gactacgtgg
    66481 cggcgaatat gaaagatgcc atcagccgta cgtctggcgt gggtgacgtt cagttgttcg
    66541 gttcacagta cgcgatgcgt atctggatga acccgaatga actgaacaaa ttccagctaa
    66601 cgccggttga tgttattacc gccatcaaag cgcagaacgc ccaggttgcg gcgggccagc
    66661 tcggtggtac accgccggtg aaaggccaac agcttaacgc ctctattatt gctcagacgc
    66721 gtctgacctc tactgaagag ttcggcaaaa tcctgctgaa agtgaatcag gatggttccc
    66781 gcgtactgct gcgtgatgtg gcgaagattg agctgggtgg tgagaactac gacatcatcg
    66841 cagagtttaa cggccaaccg gcttccggtc tggggatcaa gctggcgacc ggtgcaaacg
    66901 cgctggatac cgctgcggca atccgtgctg aactggcgaa gatggaaccg ttcttcccgt
    66961 cgggtctgaa aattgtttac ccgtacgaca ccacgccgtt cgtgaaaatc tctattcacg
    67021 aagtggttaa aacgctggtc gaagcgatca tcctcgtgtt cctggttatg tatctgttcc
    67081 tgcagaactt ccgcgcgacg ttgattccga ccattgccgt accggtggta ttgctcggga
    67141 cctttgccgt ccttgccgcc tttggcttct cgataaacac gctaacaatg ttcgggatgg
    67201 tgctcgccat cggcctgttg gtggatgacg ctatcgttgt ggtagaaaac gttgagcgtg
    67261 tgatggcgga agaaggtttg ccgccaaaag aagccacccg taagtcgatg gggcagattc
    67321 agggcgctct ggtcggtatc gcgatggtac tgtcggcggt attcgtaccg atggccttct
    67381 ttggcggttc tactggggca atttatcgtc agttctctat taccattgtt tcagcaatgg
    67441 cgctgtcggt actggtggcg ttgatcctga ctccagctct ttgtgccacc atgctgaaac
    67501 cgattgccaa aggcgatcac ggggaaggta aaaaaggctt cttcggctgg tttaaccgca
    67561 tgttcgagaa gagcacgcac cactacaccg acagcgtagg cggtattctg cgcagtacgg
    67621 ggcgttatct ggtgctgtat ctgatcatcg tggtcggcat ggcctatctg tttgtgcgtc
    67681 tgccaagctc cttcttgcca gatgaggacc agggcgtatt tatgaccatg gttcagctgc
    67741 cagcaggtgc aacgcaggaa cgtacgcaga aagtgctcaa tgaggtaacg cattactatc
    67801 tgaccaaaga aaagaacaac gttgagtcgg tgttcgccgt taacggcttc ggctttgcgg
    67861 gacgtggtca gaataccggt attgcgttcg tttccttgaa ggactgggcc gatcgtccgg
    67921 gtgaagaaaa caaagttgaa gcgattacca tgcgtgcaac acgcgctttc tcgcaaatca
    67981 aagatgcgat ggttttcgcc tttaacctgc ccgcaatcgt ggaactgggt accgcaaccg
    68041 gctttgactt tgagctgatt gaccaggctg gccttggtca cgaaaaactg actcaggcgc
    68101 gtaaccagtt gcttgcagaa gcagcgaagc accctgatat gttgaccagc gtacgtccaa
    68161 acggtctgga agataccccg cagtttaaga ttgatatcga ccaggaaaaa gcgcaggcgc
    68221 tgggtgtttc tatcaacgac attaacacca ctctgggcgc tgcatggggc ggcagctatg
    68281 tgaacgactt tatcgaccgc ggtcgtgtga agaaagttta cgtcatgtca gaagcgaaat
    68341 accgtatgct gccggatgat atcggcgact ggtatgttcg tgctgctgat ggtcagatgg
    68401 tgccattctc ggcgttctcc tcttctcgtt gggagtacgg ttcgccgcgt ctggaacgtt
    68461 acaacggcct gccatccatg gaaatcttag gccaggcggc accgggtaaa agtaccggtg
    68521 aagcaatgga gctgatggaa caactggcga gcaaactgcc taccggtgtt ggctatgact
    68581 ggacggggat gtcctatcag gaacgtctct ccggcaacca ggcaccttca ctgtacgcga
    68641 tttcgttgat tgtcgtgttc ctgtgtctgg cggcgctgta cgagagctgg tcgattccgt
    68701 tctccgttat gctggtcgtt ccgctggggg ttatcggtgc gttgctggct gccaccttcc
    68761 gtggcctgac caatgacgtt tacttccagg taggcctgct cacaaccatt gggttgtcgg
    68821 cgaagaacgc gatacttatc gtcgaattcg ccaaagactt gatggataaa gaaggtaaag
    68881 gtctgattga agcgacgctt gatgcggtgc ggatgcgttt acgtccgatc ctgatgacct
    68941 cgctggcgtt tatcctcggc gttatgccgc tggttatcag tactggtgct ggttccggcg
    69001 cgcagaacgc agtaggtacc ggtgtaatgg gcgggatggt gaccgcaacg gtactggcaa
    69061 tcttcttcgt tccggtattc tttgtggtgg ttcgccgccg ctttagccgc aagaatgaag
    69121 atatcgagca caaccatact gtcgatcatc attgatacaa cgtgtaatca ctaaggccgc
    69181 gtaagcggcc ttttttatgc ataacctacg aacattaagg agtaattgaa ccaccaactc
    69241 aggatctcat acgaaaacca gtattaacca cg
//
LOCUS       DAZFAB010000013        67398 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZFAB010000013 DAZFAB010000000
VERSION     DAZFAB010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 67398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.013"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            48..665
                     /locus_tag="ACHJWA_000993"
                     /pseudo
     CDS             48..665
                     /locus_tag="ACHJWA_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            768..1271
                     /gene="yfbM"
                     /locus_tag="ACHJWA_000994"
     CDS             768..1271
                     /gene="yfbM"
                     /locus_tag="ACHJWA_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HFO4307640.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPVKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKKFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(1338..2795)
                     /gene="nuoN"
                     /locus_tag="ACHJWA_000995"
     CDS             complement(1338..2795)
                     /gene="nuoN"
                     /locus_tag="ACHJWA_000995"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFO4307641.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2802..4331)
                     /gene="nuoM"
                     /locus_tag="ACHJWA_000996"
     CDS             complement(2802..4331)
                     /gene="nuoM"
                     /locus_tag="ACHJWA_000996"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFO4307642.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4562..6403)
                     /gene="nuoL"
                     /locus_tag="ACHJWA_000997"
     CDS             complement(4562..6403)
                     /gene="nuoL"
                     /locus_tag="ACHJWA_000997"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFO4307643.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6400..6702)
                     /gene="nuoK"
                     /locus_tag="ACHJWA_000998"
     CDS             complement(6400..6702)
                     /gene="nuoK"
                     /locus_tag="ACHJWA_000998"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFO4307644.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6699..7253)
                     /gene="nuoJ"
                     /locus_tag="ACHJWA_000999"
     CDS             complement(6699..7253)
                     /gene="nuoJ"
                     /locus_tag="ACHJWA_000999"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFO4307645.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7265..7807)
                     /gene="nuoI"
                     /locus_tag="ACHJWA_001000"
     CDS             complement(7265..7807)
                     /gene="nuoI"
                     /locus_tag="ACHJWA_001000"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFO4307646.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7822..8799)
                     /gene="nuoH"
                     /locus_tag="ACHJWA_001001"
     CDS             complement(7822..8799)
                     /gene="nuoH"
                     /locus_tag="ACHJWA_001001"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFO4307647.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8796..11522)
                     /gene="nuoG"
                     /locus_tag="ACHJWA_001002"
     CDS             complement(8796..11522)
                     /gene="nuoG"
                     /locus_tag="ACHJWA_001002"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFO4307648.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSLE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQCRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11575..12912)
                     /gene="nuoF"
                     /locus_tag="ACHJWA_001003"
     CDS             complement(11575..12912)
                     /gene="nuoF"
                     /locus_tag="ACHJWA_001003"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFO4307649.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12909..13409)
                     /gene="nuoE"
                     /locus_tag="ACHJWA_001004"
     CDS             complement(12909..13409)
                     /gene="nuoE"
                     /locus_tag="ACHJWA_001004"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFO4307650.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHANLTPEAIPE
                     LLERYK"
     gene            complement(13412..15214)
                     /gene="nuoC"
                     /locus_tag="ACHJWA_001005"
     CDS             complement(13412..15214)
                     /gene="nuoC"
                     /locus_tag="ACHJWA_001005"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311197.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFO4307651.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPARVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15308..15970)
                     /gene="nuoB"
                     /locus_tag="ACHJWA_001006"
     CDS             complement(15308..15970)
                     /gene="nuoB"
                     /locus_tag="ACHJWA_001006"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFO4307652.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15986..16429)
                     /gene="nuoA"
                     /locus_tag="ACHJWA_001007"
     CDS             complement(15986..16429)
                     /gene="nuoA"
                     /locus_tag="ACHJWA_001007"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFO4307653.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17060..17998)
                     /gene="lrhA"
                     /locus_tag="ACHJWA_001008"
     CDS             complement(17060..17998)
                     /gene="lrhA"
                     /locus_tag="ACHJWA_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFO4307654.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPISAPEGDDSLLIERDIE"
     gene            18918..20135
                     /gene="alaA"
                     /locus_tag="ACHJWA_001009"
     CDS             18918..20135
                     /gene="alaA"
                     /locus_tag="ACHJWA_001009"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFO4307655.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPCGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20219..20818
                     /gene="yfbR"
                     /locus_tag="ACHJWA_001010"
     CDS             20219..20818
                     /gene="yfbR"
                     /locus_tag="ACHJWA_001010"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFO4307656.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(20877..22709)
                     /gene="yfbS"
                     /locus_tag="ACHJWA_001011"
     CDS             complement(20877..22709)
                     /gene="yfbS"
                     /locus_tag="ACHJWA_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFO4307657.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(22796..23446)
                     /gene="hxpA"
                     /locus_tag="ACHJWA_001012"
     CDS             complement(22796..23446)
                     /gene="hxpA"
                     /locus_tag="ACHJWA_001012"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFO4307658.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23457..23951)
                     /gene="yfbU"
                     /locus_tag="ACHJWA_001013"
     CDS             complement(23457..23951)
                     /gene="yfbU"
                     /locus_tag="ACHJWA_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFO4307659.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(24034..24489)
                     /gene="yfbV"
                     /locus_tag="ACHJWA_001014"
     CDS             complement(24034..24489)
                     /gene="yfbV"
                     /locus_tag="ACHJWA_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFO4307660.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            24827..26029
                     /gene="ackA"
                     /locus_tag="ACHJWA_001015"
     CDS             24827..26029
                     /gene="ackA"
                     /locus_tag="ACHJWA_001015"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFO4307661.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26104..28248
                     /gene="pta"
                     /locus_tag="ACHJWA_001016"
     CDS             26104..28248
                     /gene="pta"
                     /locus_tag="ACHJWA_001016"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO4307662.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIDSLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28438..29958
                     /gene="yfcC"
                     /locus_tag="ACHJWA_001017"
     CDS             28438..29958
                     /gene="yfcC"
                     /locus_tag="ACHJWA_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFO4307663.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRLLTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(29991..30533)
                     /gene="yfcD"
                     /locus_tag="ACHJWA_001018"
     CDS             complement(29991..30533)
                     /gene="yfcD"
                     /locus_tag="ACHJWA_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFO4307664.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30591..31142)
                     /gene="yfcE"
                     /locus_tag="ACHJWA_001019"
     CDS             complement(30591..31142)
                     /gene="yfcE"
                     /locus_tag="ACHJWA_001019"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFO4307665.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31198..31842)
                     /gene="yfcF"
                     /locus_tag="ACHJWA_001020"
     CDS             complement(31198..31842)
                     /gene="yfcF"
                     /locus_tag="ACHJWA_001020"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFO4307666.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLLFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLVLGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPECLVDYATFQWQRASVQRFIALSAKQSG"
     gene            31978..32625
                     /gene="yfcG"
                     /locus_tag="ACHJWA_001021"
     CDS             31978..32625
                     /gene="yfcG"
                     /locus_tag="ACHJWA_001021"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFO4307667.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAGLDYRLIKVDLGKGGQFRPEFL
                     LISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            32682..33044
                     /gene="folX"
                     /locus_tag="ACHJWA_001022"
     CDS             32682..33044
                     /gene="folX"
                     /locus_tag="ACHJWA_001022"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFO4307668.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33065..33958
                     /gene="yfcH"
                     /locus_tag="ACHJWA_001023"
     CDS             33065..33958
                     /gene="yfcH"
                     /locus_tag="ACHJWA_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFO4307669.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVMTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGIDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNKFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPIRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(34006..34896)
                     /gene="rpnB"
                     /locus_tag="ACHJWA_001024"
     CDS             complement(34006..34896)
                     /gene="rpnB"
                     /locus_tag="ACHJWA_001024"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFO4307670.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYLYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDVGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLEKGFD
                     HEVILTLTRLSPDDLIAQSH"
     gene            complement(35093..35866)
                     /gene="hisP"
                     /locus_tag="ACHJWA_001025"
     CDS             complement(35093..35866)
                     /gene="hisP"
                     /locus_tag="ACHJWA_001025"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFO4307671.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(35874..36590)
                     /gene="hisM"
                     /locus_tag="ACHJWA_001026"
     CDS             complement(35874..36590)
                     /gene="hisM"
                     /locus_tag="ACHJWA_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4307672.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36587..37273)
                     /gene="hisQ"
                     /locus_tag="ACHJWA_001027"
     CDS             complement(36587..37273)
                     /gene="hisQ"
                     /locus_tag="ACHJWA_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFO4307673.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAARAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37363..38145)
                     /gene="hisJ"
                     /locus_tag="ACHJWA_001028"
     CDS             complement(37363..38145)
                     /gene="hisJ"
                     /locus_tag="ACHJWA_001028"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFO4307674.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38366..39148)
                     /gene="argT"
                     /locus_tag="ACHJWA_001029"
     CDS             complement(38366..39148)
                     /gene="argT"
                     /locus_tag="ACHJWA_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFO4307675.1"
                     /translation="MKKSILALSLLVGISAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39414..39983)
                     /gene="ubiX"
                     /locus_tag="ACHJWA_001030"
     CDS             complement(39414..39983)
                     /gene="ubiX"
                     /locus_tag="ACHJWA_001030"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFO4307676.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(40078..41595)
                     /gene="purF"
                     /locus_tag="ACHJWA_001031"
     CDS             complement(40078..41595)
                     /gene="purF"
                     /locus_tag="ACHJWA_001031"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFO4307677.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIEAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41632..42120)
                     /gene="cvpA"
                     /locus_tag="ACHJWA_001032"
     CDS             complement(41632..42120)
                     /gene="cvpA"
                     /locus_tag="ACHJWA_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFO4307678.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGLLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42652..43314)
                     /gene="dedD"
                     /locus_tag="ACHJWA_001033"
     CDS             complement(42652..43314)
                     /gene="dedD"
                     /locus_tag="ACHJWA_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFO4307679.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVVPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(43304..44572)
                     /gene="folC"
                     /locus_tag="ACHJWA_001034"
     CDS             complement(43304..44572)
                     /gene="folC"
                     /locus_tag="ACHJWA_001034"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFO4307680.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVCYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKTLPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44642..45556)
                     /gene="accD"
                     /locus_tag="ACHJWA_001035"
     CDS             complement(44642..45556)
                     /gene="accD"
                     /locus_tag="ACHJWA_001035"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFO4307681.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45712..46371)
                     /gene="dedA"
                     /locus_tag="ACHJWA_001036"
     CDS             complement(45712..46371)
                     /gene="dedA"
                     /locus_tag="ACHJWA_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO4307682.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(46454..47266)
                     /gene="truA"
                     /locus_tag="ACHJWA_001037"
     CDS             complement(46454..47266)
                     /gene="truA"
                     /locus_tag="ACHJWA_001037"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFO4307683.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(47266..48279)
                     /gene="usg"
                     /locus_tag="ACHJWA_001038"
     CDS             complement(47266..48279)
                     /gene="usg"
                     /locus_tag="ACHJWA_001038"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO4307684.1"
                     /translation="MSEGWNIAVLGTTGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFVQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48345..49481)
                     /gene="pdxB"
                     /locus_tag="ACHJWA_001039"
     CDS             complement(48345..49481)
                     /gene="pdxB"
                     /locus_tag="ACHJWA_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFO4307685.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHERTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADCGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            49580..50575
                     /gene="flk"
                     /locus_tag="ACHJWA_001040"
     CDS             49580..50575
                     /gene="flk"
                     /locus_tag="ACHJWA_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFO4307686.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHCPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMTLKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(50572..51750)
                     /gene="yfcJ"
                     /locus_tag="ACHJWA_001041"
     CDS             complement(50572..51750)
                     /gene="yfcJ"
                     /locus_tag="ACHJWA_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4307687.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALALTTMALPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(52025..53245)
                     /gene="fabB"
                     /locus_tag="ACHJWA_001042"
     CDS             complement(52025..53245)
                     /gene="fabB"
                     /locus_tag="ACHJWA_001042"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFO4307688.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            53404..55410
                     /gene="mnmC"
                     /locus_tag="ACHJWA_001043"
     CDS             53404..55410
                     /gene="mnmC"
                     /locus_tag="ACHJWA_001043"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFO4307689.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEARFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     ISQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNGFTFAR
                     RLYDSLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPEELAVAVEANAVEQITGVT
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQDLSRKDDCWLLTFAGDQ
                     QATHSVVVLANGHQISRFSQTSSLPVYSVAGQVSHIPTTPELAKLKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEETAYSEDNQQQNRQRLIDCFPHAQWAKTVDVSKKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDKAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(55531..55809)
                     /gene="yfcL"
                     /locus_tag="ACHJWA_001044"
     CDS             complement(55531..55809)
                     /gene="yfcL"
                     /locus_tag="ACHJWA_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFO4307690.1"
                     /translation="MIAEFESRILELIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(55843..56391)
                     /gene="epmC"
                     /locus_tag="ACHJWA_001045"
     CDS             complement(55843..56391)
                     /gene="epmC"
                     /locus_tag="ACHJWA_001045"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFO4307691.1"
                     /translation="MNSTHHYEQLIEIFNSCFADDFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARREQVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(56391..57200)
                     /gene="yfcA"
                     /locus_tag="ACHJWA_001046"
     CDS             complement(56391..57200)
                     /gene="yfcA"
                     /locus_tag="ACHJWA_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFO4307692.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(57200..58024)
                     /gene="mepA"
                     /locus_tag="ACHJWA_001047"
     CDS             complement(57200..58024)
                     /gene="mepA"
                     /locus_tag="ACHJWA_001047"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFO4307693.1"
                     /translation="MNKTAIALLALLASSVSLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRRTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(58028..59113)
                     /gene="aroC"
                     /locus_tag="ACHJWA_001048"
     CDS             complement(58028..59113)
                     /gene="aroC"
                     /locus_tag="ACHJWA_001048"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFO4307694.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSQVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(59148..60080)
                     /gene="prmB"
                     /locus_tag="ACHJWA_001049"
     CDS             complement(59148..60080)
                     /gene="prmB"
                     /locus_tag="ACHJWA_001049"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO4307695.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKAQLLAAREHFAIYKD"
     gene            60246..60797
                     /gene="smrB"
                     /locus_tag="ACHJWA_001050"
     CDS             60246..60797
                     /gene="smrB"
                     /locus_tag="ACHJWA_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311241.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFO4307696.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKVSEVPV
                     KRLIQEQVDASHYFSDEFQPLLNADGPVKYVRPGVDHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60968..61810)
                     /locus_tag="ACHJWA_001051"
     CDS             complement(60968..61810)
                     /locus_tag="ACHJWA_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFO4307697.1"
                     /translation="MKKWTIFLTSLILLALSLETPKCYAGDKLMSASFSSTKIYYAMK
                     NVTASGSLYFYVTVVTPGEVSYGQYNSNARKGDTLKLISWSGSGPAPTLVLTDYRRTD
                     TSNCPGINTRVFVCAYMTFNVTVESDNYGCPWIASFYSVSEAFGFGTYTSPTVHDSIC
                     PTIPVASFDISWSDNYVSHNKALRLQSDGSTITTTLSTYLMESGKLCDGSIFDSRGAY
                     CRAVSDLLTFTSYGCDNAKVTVTPSRQPLTDRKLHDIVVQVNTSSREPIDSTCRFQYV
                     LNEL"
     gene            complement(61812..62333)
                     /locus_tag="ACHJWA_001052"
     CDS             complement(61812..62333)
                     /locus_tag="ACHJWA_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4307698.1"
                     /translation="MKNNRAWALISGLILFSGTAPAADNLHFTGNLLGKSCTPVINGN
                     LLAEIHFPTIAASDLMQRGQSDRVPLVFQLKDCKSTTAFNVKVTLMGTEDTDLPGFLS
                     IDSSSSATGVGIGIETAGGAAVPINSTTGASFPLNQGNNSVNFNAWLQTVNGRNVTSG
                     DFTATMTVTFEYF"
     gene            complement(62330..62800)
                     /locus_tag="ACHJWA_001053"
     CDS             complement(62330..62800)
                     /locus_tag="ACHJWA_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4307699.1"
                     /translation="MKRISLLMLWSFSFMALSNVSFHGYLVQPPNCSISNGQNIELTF
                     RDVNIDDINGSNYEQVVPYTITCDTAVRDPQMEMTLTWSGTQSDFDDSAVATDLNGLG
                     IHLKQAGSDFKLHTPLVVNETSLPVLTAVPVKKSGVDLPESDFEAWATLQVDYQ"
     gene            complement(62797..63297)
                     /locus_tag="ACHJWA_001054"
     CDS             complement(62797..63297)
                     /locus_tag="ACHJWA_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4307700.1"
                     /translation="MKGAYSAWAWLLLCSIVSTPVMAGSKSVAMVLRVLVDAPPPCTV
                     TGASVEFGNVFISKIDGVSYKRPIDYSLVCNNLAMDDLRLNMQATTTVINGETVIDTG
                     IAGFGIRIQKVSDHSILDLTPGAWLPFNFSSGALALEAVPVVQSGVSLTAAEFSASAT
                     IVVDYQ"
     gene            complement(63308..64066)
                     /locus_tag="ACHJWA_001055"
     CDS             complement(63308..64066)
                     /locus_tag="ACHJWA_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO4307701.1"
                     /translation="MTNLFCSARTAATTLALLITAASLSVQASVTPDRTRLIFNESDK
                     SISVTLRNNDPKMPYLAQSWMEDGQGNRISSPLTVLPPVQRIDSMMNGQVKVQGMPDI
                     NKLPADRESVFYFNVREIPPKSNKPNTLQIALQTRIKLFWRPKALENVSMKNPWQHKV
                     TLTLSGQEFTVNNPTPYFVVISNASAQKNGNPAAGFSPMVISPKTSVPLKVKMDSVPV
                     LTYVNDYGARLPLFFRCTGNTCQVDEEQNSKGQG"
     gene            complement(64089..66728)
                     /locus_tag="ACHJWA_001056"
     CDS             complement(64089..66728)
                     /locus_tag="ACHJWA_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFO4307702.1"
                     /translation="MPDHSLFRLRVLPCCVALAMSGSYVNAWAENEIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLTEEYDIYWYASENDASKTYACLTPELV
                     AQFGLKEDVAKNLQWIHDGKCLKPGQLEGIDIKADLSQSALVISLPQAYLEYTDINWD
                     PPSRWDDGISGLIADYSITAQTRHEENGGDDSNEISGNGTVGVNLGAWRLRADWQTDY
                     LHSKSNDDDVINGDDTQKNWEWSRYYAWRALPSLKAKLGLGEDYLNSDIFDGFNYVGG
                     SISTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQLGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDINTASMPFLTRPGQVRYKLMMGRPQEWGHHVEGGFFSGG
                     EASWGIANGWSLYGGALADEHYQSAALGVGRDLSVFGAVAFDITHSHTRLDKETAYGK
                     GSLDGNSFRLSYSKDFDELNSRVTFAGYRFSEENFMTMSEYLDASDSEMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDRDEQTNYNVMLSHYFNLGSIRNMSISMTGYR
                     YEYDNQADKGVYISLSMPWGDSSTISYNGNYGSGSDSSQVGYFSRVDDATHYQLNVGT
                     SDNHSSVDGYYSHDGSLAQVDLSANYHEGQYTSAGISLQGGATLTAQGGALHRTQNMG
                     GTRLLIDADGVAGVPVEGNGAAVYTNMFGKAVVADVNNYYRNQAYIDLNNLPENAEAT
                     QSVVQATLTEGAIGYRKFSVISGQKAMAVLRLQDGSYPPFGAEVKNDSAQNVGLVDDD
                     GNVYLAGVKPGEHMIVSWGGVAHCDIHLPDPLPADLFNGLLLPCQQTGAISPSMPHEI
                     KPVIQEQTQQVMPTEAPVSVSAN"
     gene            complement(66810..67373)
                     /gene="yfcV"
                     /locus_tag="ACHJWA_001057"
     CDS             complement(66810..67373)
                     /gene="yfcV"
                     /locus_tag="ACHJWA_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4307703.1"
                     /translation="MSKFVKTAIAATMVMGAFASTSTIAAGNNGTARFYGTIEDSPCS
                     IVPDDHKLEVDMGDIGSGILKNNGTSTPKAFQIHLQDCVFDTQTTMTTTFTGNASSTN
                     SGNYYTIYNTDTGAAFNNVSLAIGDAQGTSYKSGAGIEQKIVNDTATNKGKAKQTLDF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        16636 a        18314 c        16928 g        15520 t
ORIGIN      
        1 acgtctttta ttgataattt acatatgaaa aaaattttcc ttttgttata ttatttctgt
       61 tttcactccc tctgattatt ttttaccaac cctgggtcac tgcattatcg ccaacgcccc
      121 gacacgtaat tcctgagcaa ttagaaaaaa cagttcgtta tcttacacaa actgtgcatc
      181 cacgtaatgc cgacaatatt gataatctga ataggtccgt agagtatata aaaaaaggtc
      241 tttgtcagta gcggtgccag agttaccgcg cagggcgtcc ccaattggct attacgactc
      301 tgcgcctggt agccaggatt acccttaccc gcaatgtcct ggctttatcc cgatcggggg
      361 gactttattg cctggtcggc agaatacagg atatcaacgc cgttcgtcag gtaaaaacgg
      421 cattgttatc atcccgggat ttatctgttt attctatgaa tgccccatgg tttattcacg
      481 gtattgattt ctctgaccac cttaattatt ggcaacacga tattctcgcc gtaatgatta
      541 ctgacaccgc tttttatcgt aataaacaat accacttgcc cggtgataca gcagacagat
      601 tgaattatca gaaaatggct cagatggtgg atggtgttat aactttgtta cacaacagta
      661 aataataaaa atttctcgag gcgggtccag aaacagcgac atgtacattt tattttccct
      721 cgtctgtttt aagatgcgaa acataaaatt caatggagga aaaataaatg ggaatgattg
      781 gttattttgc agaaattgac tcagaaaaga ttaaccaact gcttgagtct actgaaaaac
      841 ctttgatgga taatatacat gacacgcttt caggattacg ccgactggat attgataaac
      901 gctgggattt tttacacttt gggctgacag gcacctctgc gttcgatcca gtgaagaatg
      961 atccgctgag tcgtgccgtg cttggtgaac acagcctcga agatggtata gatggtttcc
     1021 tgggattgac atggaatcag gagttagccg cgacaatcga ccgactggaa tcactggatc
     1081 gaagtgagct gcgtaaaaag ttttccatta agcgactcaa tgaaatggag atttatccgg
     1141 gagtgacctt ttcagaagag ttagaggggc aactctttgc ttcgatcatg cttgatatgg
     1201 aaaagctgat tagcgcgtat cgacgtatgc tgcgacaagg gaatcatgcg ctgacggtta
     1261 ttgttgggtg atggtgtcag ggcaccaaaa aaaaccgccg aacatgtcgg cggctttctg
     1321 acttacaaac tgatagctta catcagcggc attgctaacc gcacaatact aatcagcggt
     1381 tgtggccata cacccagcac cagtaccaac agtgcagaaa tcagcaccac gataccgccc
     1441 gcgctgtact gccagtttga cggtgcatcg cgacccggtt gttccggtgc gtgcaggtac
     1501 aggctcaccg ccacgcgcag gtagtagtag aggccgattg ccgaaccgac aaccacggca
     1561 cccaccagcc accacaggtg tgcctggaca ccaaccgcca gcacgtagaa cttaccgata
     1621 aagcccagcg tcatcgggat accggccaga gacagcatca tcaccgtcat caccgccgcg
     1681 aggatcggac gatgccagaa cagaccgcgg taagagaaca gggaatcagc atccgggcca
     1741 cgatacgggc tggacatcag gctgaccacg ccgaacgcgc cgaggctgct gaacagataa
     1801 ccggccaggt aaacccctac cgcttccatc gacatctcgc cagtttgcag cgcaatcagc
     1861 gccaccagca gatagccgag gtgagagata gatgagtaac cgagcagacg tttgatattg
     1921 gtctggctca gcgccatcag gttaccgaag atgatggagg caaaggcgat aatcgccagc
     1981 accacccgaa tcgcttcgct gtcacccacc ggtgcgtaga ggaacagacg catcaccaca
     2041 ccgaagatag cgattttgct cgccgtcgcc aggaaagtgg aaaccggcgc aggcgcgccc
     2101 tggtatacgt ctggcgtcca caggtggaac ggcaccagag agagtttgaa gccgaggcca
     2161 acaatcatca ggccgaaacc tgccagcaac agcggctcgt ttagcatacc gtcgccgagg
     2221 tttttaccca gcgcgacaaa cgacaggtcg ccagactgcg catacaccag cgccataccg
     2281 aacagcagga aagaagacgc tgcggcagaa aggatggtgt atttgatact ggcttccagc
     2341 gaacgtttct ggcggaaagc gtaaccgacc aggccaaaca gcggcaaaga gatcagttca
     2401 ataccgagga acagagacgc cagatggttt gcattcgcca gcaggatccc gcccagcgcg
     2461 gcaattaaca ccaacaggta gaactcatcc ttgttgtcgt tatagccttc aagccacggg
     2521 taggcgaaag tacaggtggc gaggctcgcc aacaatacca gcccggtgta aagcatggcg
     2581 aaaccatcaa cgcgcatcag cggcgtaacg tccatagcgc ccgcctggcc aacaaaccag
     2641 agcgaaacca gcgccgcgtt aagcccaata accgagagcg tagcgttgag gaaatgattg
     2701 cgtcgccacg caatggagag catcacaacc accaccgtca agccgacgat cagcaacggt
     2761 agcagtgcga tcaggttttg tggagttatt gtcatggcga tttacggcct tgtagtagta
     2821 acggaattaa caaaccactg ctggatattg ccaatcgcgg agtgcgaggt atccagaatc
     2881 ggctgcggat agaagcccag cagtaccagc agcaccacca gcaacaggat cataaacagc
     2941 tcacgcagcg acatccctgg cagttcctgg ctggcaatct ggcttttcgc tttaccgaag
     3001 taagcgcgat gtaacatcgc cagcgaataa acagatgcaa agaccagccc aaaggtagag
     3061 ataacggtaa tcaccgggac aacctggaag ctgccgaaca gaatcataaa ttcgccgacg
     3121 aagttaccgg tgcctggcat cccgagcgtt gccaccgcaa agaacagtga caaggctggc
     3181 agccatttca tcttgctcca cagaccgccc atcatgcgca tgtcgcgggt atggatacgt
     3241 tcataaagct gaccacaaag aataaacaga cccgccgctg acaagccgtg cgcaatcatc
     3301 tggattaccg cgccctggta ggccaactgg ctgccggtgt agatagcaat cagtacgaag
     3361 cccatgtggg aaaccgaggt gtaggcgatc agacgtttga tatcggtctg ggcgaaggcc
     3421 atccacgcac cgtagaagat gccgataaca cccagccaca tggcgattgg cgcgaactct
     3481 gccgacgcgt ttgggaacag cggtagggag aaacgcagca gaccgtaagc ggcggttttc
     3541 agcaagatcc ccgcgaggtc cacggaaccg gcggtcggtg cctgtgagtg cgcatccggc
     3601 agccagccat gcagcggaac caccggcatt ttcaccgcaa acgcgatgaa gaagcccagc
     3661 atcaacagat attccacacc actggacatt ggcgtattca gcagctcttc atagttgaag
     3721 gtccagacgc cggtcgcatt gtagtgaaca aaaaccagcg ccaggatggc gatcaacatc
     3781 accagaccac tcgcctgggt gtaaatgaag aacttggttg ccgccgtgat acgcgtttta
     3841 ccatcagagg ctttatgccc ccacagtgcg atcaggaagt acatcggcac cagcatcatt
     3901 tcccagaaga agaagaacag gaacatgtcg atggcaagga acacgccgat aacgccgccc
     3961 aggatccaca tcaggttgag gtggaagaag ccctgatatt tttcgatctc tttccacgaa
     4021 cagagtaccg ccagcacacc gagcagaccg gtcagcacga ccatcagcag cgacagcccg
     4081 tcaatggcga gatggataga gataccaaaa cgcgggatcc acggcatgtc gaattcagac
     4141 tgccactgcg gaattccggc ggattgcgtc agtgaataac cgccctgcaa ccacagttgc
     4201 agcgacagcg ccagcgtcaa tcccatggtg atcagcgcga tccagcgcgg caccttgacg
     4261 ccaaagcgtt cggtctgcca gcacaggaag ccgccgataa aggggattag tattaaccag
     4321 ggtaataaca tagctaagta tgtcccttat atttactcct gctcaggcta tctcactcgc
     4381 cagcccgaat cccggcagtg ctcgcaatcc tcacgtactt aagtacgctc cggttgctgt
     4441 gcgctggcgg tatccggtct gacttcgccg atgacgcctg tacttcagga acagttacct
     4501 aaaatttatt cttagttcac agacccggcg caacgccggg tatattcact cagccaacaa
     4561 ctcaacgcag taccatcaac agtgccagca ccacgaccgc accgatgctc atggatgcca
     4621 cataccagcg cagataaccg ttctcgctta acagcaggcc tttacctgca aagcgggaaa
     4681 ggacagccgg gatgttcatc attgagttca gcggatcgcg tttcagcaac caggcaatac
     4741 ccaggaacgg cttgacgaac actttgtcgt acagccagtc aaagccccag gcgttgtacc
     4801 accaggtgcc cagcagacgg cccggcgcac tgttggcgat ggaggtcacc agagtacgtt
     4861 tacccagcca cagccaggct gccagcagaa tgccgaccac cgcgaccacg ccagaggtaa
     4921 tttccagggt caacatgctg ccgtgcgcca gttccgtcgt ttgcggaagc acgccctgca
     4981 gcggcggtac aatcagtgcg ccaacgaagg tggaaaggat cagcagcaca atcagcggca
     5041 ggctgtgagt tacccctttc acggcgtgag cgtgaatttg ttcttttccg tggaagacga
     5101 tgaaaatcat acggaaggtg tagagcgagg tcataaacgc accgaccaga cctgccacca
     5161 tcagattgat atgaccattc gccatcgcac ccgcgaggat ctcatcctta ctgaagaagc
     5221 ccgcagtgac tagcggcagt gccgacagtg ctgcgccgcc caccaggaag cagagataaa
     5281 ccagcggaat agatttacgc agaccgccca tcttgaagat gttctgttcg tgatggcagg
     5341 cgagaatgac ggaaccggat gccaggaaca gcagcgcttt aaagaacgcg tgggtcatca
     5401 agtgaaaaat cgccgcatcc catgcctgca cgccaagcgc gaggaacatg tagccaatct
     5461 ggctcatggt agagtaagcg agaacacgtt tgatgtcggt ctgtaccagc gcggcaaaac
     5521 cggccagcag cagcgtaacc gccccaacaa tgcccaccag atgcagaact tccggcgtca
     5581 tcaggaacag gccgtgggta cgggcgatca ggtagacacc cgcggttacc atcgttgcgg
     5641 cgtggatcag cgcggagaca ggcgtcgggc ccgccatcgc gtcggcaagc catgtctgca
     5701 acggcaactg cgcagattta ccgaccgcac cgcccagcag catcagcgtc gcccacatca
     5761 gcatgttatt gccgtcagca aagtgcgctg gtgccagttc caccatttcg cggaagttca
     5821 gggtgcccag ttcgttgtaa agaatgaaca gcgcgaaagc gaggaacacg tcacccacac
     5881 gggtcacgac gaacgctttc attgccgctg cgccattctt cggatcggtg taatagaacc
     5941 cgatcagcag ataggagcac aggcccacgc cttcccagcc gaggtacatc agcagcaggt
     6001 tgtcggcaag caccagaacc accatgctgg cgataaacag gttggtgtaa gcgaagaagc
     6061 gagagtagcc ctcttcaccg cgcatatacc aggaggcgta catgtgaata aggaaaccca
     6121 caccagtgac caccgagagc atggtcagcg acaggccgtc cagcaccagg ttaaaaccga
     6181 tgttaaagtc gcctaccgac atccacgtcc acagcggctg gctgtatgcc tgctcgccgt
     6241 tagcgaagaa gtcaacgcca ataaaggcag ttaccagcgc cgccaggccc acagagccaa
     6301 cgccgacgat cgccgagacg ttttcagacc agcgcccacg ggagaacgcc agcaggacga
     6361 agccaatcaa tggcaaaata atggttaagg caagcatgtt catccgcgca tctcacttac
     6421 tgaatcgatg ttcaggttct ggcgacgacg gtgaagttgc agcagcagcg caaggccgat
     6481 actcgcttct gccgccgcga ggctgatggc gagaatgtac atcacctgac cgtcggtctg
     6541 gccccagtaa cttccggcga ccacaaaggc cagcgcggaa gcgttaatca tgatttccag
     6601 accaatcagc ataaacagca gattgcgacg gataaccaga ccggttaagc caagaacgaa
     6661 taagattgcc gcgaggatca gtccatgttg taaggggatc atgcgtgctc ctccgttttt
     6721 cttttcgcgc tgtcgtcttt acgattgctc agcacttcac ccgcacgctc ttcacgaccg
     6781 acgtggaagg ccacaaccag acccgcgagc agcagcatag aagccagttc caccgccagt
     6841 acgtaaggcc cgaacagcgt aatacccact gctttagcac tgattggcgt accgtcgata
     6901 ccttgatcgt taacaccgag aatggcgtaa acaatcacca ccaacatgat ggctgacaaa
     6961 attgccggac caatccacac ctgcggtttc agccactggc gttcttgttc gatttctgaa
     7021 ccgcccaggt tgagcatcat caccacgaac acgaacagca ccataatggc acccgcgtag
     7081 acgataattt ccagcgcacc ggcgaagtaa gcgcctagtg agaagaacac cccggaaatc
     7141 gccagcagcg aaataatcag gtacagcagt gcgtgtaccg gattggtatg ggtgatcact
     7201 cgcaaggtcg caagtatggc tatcaggcca cagatataaa aagcgaactc cattgcccct
     7261 ctccttacgg taacaggctc ttgacgtcga taggcttggc ttcgttctct gcttcgccct
     7321 tatctttgcc gtcgattgcc atacctgcca tccggtagaa gttatactcc gggtatttgc
     7381 ccggaccgga gatcagcaga tcctctttct cgtaaaccag atcctggcgc ttgtattccc
     7441 ccatttcgaa atccggggtt aactgaatcg ccgtggtcgg acaggcttct tcgcacaaac
     7501 cacagaaaat gcagcgtgag aagttgatgc ggaaaaattc cggataccag cgaccgtctt
     7561 tggtttctgc tttttgcagc gagatacagc cgaccgggca ggctaccgcg cagaggttac
     7621 aggctacgca acgctcttcg ccgtccgggt cgcgggtcag aacgatacga ccacgataac
     7681 ggggcggcag atagaccggc tcttccgggt acattcgcgt ttcgcgtttg gcgaacgcgt
     7741 gcaggccgat catccagata ctacgaacct gggtgccgaa acctactaac aattctttta
     7801 aggtcatggt cttattgccc cttattgcgc ctgccagaga atgacagccg ccgttaccag
     7861 caagttgatc agcgtcagcg gcaggcagat tttccagccg aaggacatta cctggtcata
     7921 acgcggacgc ggtaacgacg cacgaatcaa aatgaacatc atcataaaga acgcggtttt
     7981 cagcgcgaac cagatgaatg gcggtaacaa cgggccttgc cagccaccga agaacagcgt
     8041 caccatcaat gcagagatgg tcacaatccc gatgtactca cccacgaaga acagaccgaa
     8101 cttcataccg gaatattcaa tgtggtaacc atccgccagt tcctgctcgg cttccggctg
     8161 gtcaaacggg tgacggtgac acaccgccac gcccgcgatg gcaaaggtaa taaaaccaaa
     8221 gaattgcggg atgacgttcc acacatgcgc ctggctgttg acgatgtcgg tcatgttgaa
     8281 tgaaccggcc tgcgccacca cgcccatcag ggaaagcccg aggaacactt cgtagctcag
     8341 ggtctgcgca gaagcacgca tcgcaccgag cagcgagtat ttgttgttac ttgaccagcc
     8401 cgcaaacagc accgcgtaaa ccgccagacc ggccatcatc aggaagaaca aaatcccgat
     8461 gttcaggtcg gcaaccaccc aacccggact gactggcaca atcgcaaagg ccagcagcag
     8521 cgaggtaaag gcaatcatcg gtgccagggt aaagatgacg cgatccgaga atttcgggat
     8581 ccagtcttct ttaaagaaca ttttgatcat gtccgcaacc agctggagcg aaccacccca
     8641 gccaacacgg ttaggtccgt aacggttctg gaacagaccc agcaggcgac gttcgccaaa
     8701 gctcatgaat gccccgcagg tgacaaccac cagcaggatc accaccgctt tgaggatggt
     8761 cagcaggatc tcaatcagtt ccggtgatat ccaactcatt gttgtgcctc cttgagatcc
     8821 tcaagatgcg cgccagccag caccggagca atgccggaca tacccatcgg caagcccacc
     8881 tgccctgccg tcagcccttc agcgatttca accggcagag tgaccgtgtt gccatcgtaa
     8941 ctaaaggaga cgcgtgtacc tgcgtttaca cccaacttcg cggcatccgc tgggttgagt
     9001 ttgatgtacg gctgcggcat acgacactgg aagaccggag cacgctgtga caattcatcg
     9061 ctgccaaaca ggtggtagta cggcgcgata cgccatttcc cgtcctgcgg ctggaagcgt
     9121 gccggtacgc tggtgaagta atccagacca ttttcgctgg tttcaaacag acgcacaccc
     9181 ggatcgccaa agcgcagttt gccgcccact tcgtcctgga atttgttcca cgcctgcggg
     9241 gagttccagc ccggagccca ggcaaacggc acttgcgaac ggtgcgcggt cggctggttg
     9301 ttaccttcca tcgagaaggt gaacatggtg tcaatatcct gcggctgacg cggctcatga
     9361 acgctgatat tggcgcgcat ggcggtacga ccgctgtaac ggtgcggttc acgggccagt
     9421 ttctgcccac gaatacggaa tgtcgcatct ggcgcagcat ctttgatacc cgccagttcc
     9481 gggattttcg ccacaacagc gtcaattaca tggtcgagct gcgtccagtc cacttcacgg
     9541 ctcagcaagg tgctgtgcag cgagtgcagc cagcgccaac tttccagcat gacagttttg
     9601 ctgtcgtaat aagccggatc gtaaacctgg aagaaacgtt gggcgcgacc ttcattatta
     9661 attaccgtac cgtcgctttc agcaaagctg gcagcggaaa gcaccagatg ggcgttttcc
     9721 atgatcgctg tgcgttgatg gtcaaccacc atcaccagcg gcgctttagc cagcgcagca
     9781 ttcacgcggg tagcagaagc gtgacgatgc agatcgtttt ccagcaccac caccgcgtcg
     9841 gcgcgtccgg tttccagttc ggttaacgct tcttcaagcg atccaccgcc cataatgccc
     9901 aaccccatgc tgttgacgga acgggcaatc atggtgatac cgacgtcagc gccgcgacct
     9961 ttcagggctt tcgcgacgtt agccgcagcc tgaatcacct ctaagctacc ggcgttcgtc
    10021 ccggagataa tcaacggttt cttcgcaccc gccagtgcct gcacgatgac gtcgattttg
    10081 ctttgcagct caggttcaat accgtcaacc gctggcgcag agttatccag cgcatgggcg
    10141 atggcaaaac ctaaacgcgc ctgatcttca accggtgcgc ggtaagtcca cgccgcgata
    10201 tcatccagac gggtgtcatc aacgttggta acaaacagcg gatgcttagc acgttgaccg
    10261 atattgagga ttgccgcaat ctgccagtca gccactttct gtgctgccgc catttcgcgc
    10321 gctttacctt tcaccgcctg gcgcactgcc agcgcgacgc gcgcgccggt ctgggtaacg
    10381 tcttcgccca gcaccagtac cgcatcgtaa gattcgattt cgcgcagagc cggagtataa
    10441 atgccgcctt cacgcagcac tttcagcgcc agttgcagac gttcctgctc accgtgagcg
    10501 ataccggtgt agaagttttc ttcgcccacc agttcacgca gcgcaaagtt gctttccacg
    10561 ctggcacgcg gagaaccaat accgatcact ttcttcgact gacgcagaat atctgccgcg
    10621 ccctgcattg cctgttcggc gttgagggta atgaaatcat cgccacgacg ctgtactggc
    10681 tgacgcggac gatctttcag gttgacgtaa ccgtaaccga aacgaccacg gtcgcagagg
    10741 aagtagtggt ttaccgtacc gttgtaacgg ttttcgatac gacgcagttc gccataacgc
    10801 tcgccggggc tgatgttaca gccgatggaa cattgctggc agatgctcgg cgcaaactgc
    10861 atatcccatt tacggttgta acgctcggag tgcgttttgt cggtaaatac gccggtcggg
    10921 caaatttcga ccaggttacc ggaaaattcg ctttccagcg tgccgtcttc cgggcgaccg
    10981 aagtagacgt tgtcatgcgc gccataaacg cccagatctg taccgtcagc gtaatctttg
    11041 tagtaacgca cgcagcggta gcaggcgatg cagcggttca tttcgtgaga gatgaacggc
    11101 cccaaatcct gattacggtg ggtacgtttg gtgaaacggt agcgacggaa gctgtgtccg
    11161 gtcatcacgg tcatatcctg aagatggcag ttaccgcctt cttcacatac cggacagtcg
    11221 tgcgggtggt tggtcattaa ccactcgacc acactttcac ggaactgttt cgcttcttcg
    11281 tcgtcaatgg aaataaaggt gccatcggaa gccggtgtca tacaggacat caccaggcga
    11341 ccacgcgtgt cttccgcgtt ttggtattgc ttcaccgcac actggcggca agcaccgacg
    11401 cttcccagcg ccggatgcca gcaaaagtaa ggaatatcaa ggcccagaga cagacaagct
    11461 tccagcaggt tgtccgctcc gttgacctcg tattctttgc cgtctacatg aattgtagcc
    11521 attagcatgc ttccagtttt ctcagtcaga gactgagcgt taatcgaaat tcggttacca
    11581 gcgctctttc agcaggttcg gctgaatccc attaatcaaa tgggtattgc tgaacggctg
    11641 tttgatcccc gcctcaaatt cttcgcggaa atatttgatg gcgctctgta acggctccac
    11701 agcaccaggt gcgtgggcac agaaagtttt acccgggcct aagaatcgac acagttgctc
    11761 aagtgtttcg atatcgcccg gctgaccttc gccacgctcc agcgcacgca gaattttcac
    11821 gctccacggc agaccgtcgc ggcacggcgt acaccagccg caggactcac gggcgaaaaa
    11881 ctcttccagg ttacgcacca gcgacaccat gttgatttca tggtcaaccg ccatcgccag
    11941 cgccgtaccc agacggctgc ccgctttacc gatactttcg aattccatcg gcaggtcaag
    12001 gtgcgcttcg gtcaggaagt cggtgcccgc gccgcctggc tgccaggctt tgaatttcag
    12061 accgtcacgc ataccaccgg cgtaatcttc gaggatctcg cgtgcggtgg tgccgaacgg
    12121 cagttcccac agtcccggat ttttcacccg accggagaag cccatcagct tggtgccagc
    12181 atctttactt ttcgagatgt tctgatacca ctccacgccg ttagcgagga tcgccggaac
    12241 gttacacagg gtttcgacgt tgttgacaca ggtcggtttg ccccatacgc cggaggttgc
    12301 cgggaagggg ggtttcgagc gtgggttagc acgacgccct tccagggagt tgattaacgc
    12361 tgtttcttcc ccgcagatgt agcgccctgc cccggtatgg acgaacagtt cgaagtcgaa
    12421 acctgttccc ataatgtttt tgccaagcaa tcccgcttca gtcgcttcgg caatggcgcg
    12481 gcgcagattt actgccgctt caatatattc gccacgcagg aagatgtagc cacggtaagc
    12541 tttcagcgca aacgcggaga tgagcatacc ttccaccagc aggtgcggca gttgctccat
    12601 caacaggcgg tctttatagg tgcccggctc catttcatcg gcgttacaca gcaggtaacg
    12661 gatgttcatg gattcgtctt tcggcatcag gctccacttc aagccagtcg aaaagcccgc
    12721 gccgccgcgc cctttcagac cagcgtcttt tacctgatta acgatttcgt ccggagacag
    12781 cccggtcagc gccttacgcg ccccttcgta accgtttttg ctgcggtatt cgtccagcca
    12841 cactggctgt ttgtcatcgc gcagacgcca ggtcagcgga tgcgtttcgg gagtacggat
    12901 aatgtttttc atttataccg ctccagcagt tcagggatcg cttccggggt cagattcgcg
    12961 tgagtgtcct catcgatcat catgtttggc cctttatcac agttccccag gcagcaagtt
    13021 ggcagcagcg taaagcggcc atcaaatgtc gtttgccctg gtttgatgtt cagctttttc
    13081 tcgagcgccg cctgaatacc ctgataccca ttgatatgac agaccacgct gtcgcaataa
    13141 cggatcacat ggcggccaac cggctggcgg aagatctggc tgtagaacgt tgccacgcct
    13201 tcgacgtcgc ttgccggaat acccagcaca tcggcgatcg cgtggatcgc accatccggc
    13261 acccagccac gctgcttctg aacgattttc agcgcttcaa tggacgccgc acgcgggtct
    13321 tcgtagtggt gcatctcgtg ctcgatcgct tcacgctctg ccgcactcag ctcaaaagcc
    13381 tcggtttgtg gttgttgatt ctcgtgcata attagcggtc cacatctgac ataacaaaat
    13441 cgatactgcc cagataaaca atcaggtcag acaccaggct gccgcggatc gccgccggaa
    13501 tttgctgcaa atgcgcataa ctcggcgtgc ggatacgagt gcgataactc atggtgctac
    13561 cgtcgctggt caggtagtaa ctgttgatcc ctttggtcgc ctcaatcatc tggaaggatt
    13621 cattggcagg catcaccggc ccccacgaca cttgcaggaa gtgggtgatc agggtttcga
    13681 tatgttgcag cgtgcgctct ttcggcggcg gcgtggtcag cgggtgatcc gctttgaacg
    13741 ggccttccgg catgttgttg aggcactgct caagaatgcg cagactctgg cgcagctctt
    13801 ccactttaag cattacgcgg gtgtagcagt cagaaacacc accaccaacc gggatttcaa
    13861 agtcgaagtt ttcatagcca gaataaggac gcgccttacg cacgtcgaag tcgatcccgg
    13921 tagcacgcag gcccgcgcca gtggtgcccc actccagcgc ctctttcgcg ccataggcgg
    13981 caacgccctg ggaacgacct ttcagaatgg tattttgcag cgccgctttc tcgtaagacg
    14041 ccagacgttt cggcatccag tcgaggaact cacgcagcag gcgatcccag ccgcgcggca
    14101 ggtcgtgcgc tacgccgcca atacggaacc acgccgggtg catacggaaa cccgtgatcg
    14161 cttccaccag atcgtaaatt ttctgacgat cggtaaaggc gaagaacacg ggcgtcattg
    14221 cgccgacgtc ctgaataaag gtcgagatat acagcaggtg gctgttgatg cggaacagtt
    14281 cggagagcat tacgcgaatg acgttaacgc gagccggcac ggtgatcccg gccagtttct
    14341 ctaccgccag cacgtaaggc atttcgttaa cgcagccgcc gaggtattcg atacggtcgg
    14401 tatacggaat gtagctgtgc caggactggc gttcgcccat tttctccgca ccacggtggt
    14461 ggtagccgat gtctggtacg cagtcgacaa tctcttcgcc gtcgagctgc aaaacgatac
    14521 ggaaagcacc gtgcgccgac gggtggttcg gaccgaggtt gaggaacatg aagtcctcgt
    14581 tttcggtgcc gcgcttcatc ccccactctt ccggtttgaa ggtcagggct tccatctcca
    14641 gatcctgttt ggctttggtc agctcaaacg gcgagaattc ggtagcgcgc gccgggtaat
    14701 ctttacgcag cgggtgacct ttccaggttt gcggcatcat gatgcggcgc aggttcgggt
    14761 gaccgtcgaa tgtaatgcca aacagatccc aggtttcacg ctcataccag ttagcgttcg
    14821 ggaacagttt ggtgaaggtc ggtacgtgca ggtcgttttc tgccagcgcc accttcagca
    14881 tgatgtcgcg gttacgatcg atagaaatca gatggtagaa aacggaaaaa tccgcggcag
    14941 gtaacccttc gcggtgcgtg cgcagacgtt cgtccatgcc gtgtaagtca aacagcatga
    15001 cgtaaggttt cggcagtttc tttaagaaat cgccaacttc cagtaattgt tcacgcttga
    15061 tccacacaac gggaaccccg gtgcgagtcg cctgaacagt aaaggcatcc ggcccaaaac
    15121 ggttgcgcag ttcgccaatt accggatcat caagatgatc gcgggtctgc caggcgggtt
    15181 cttgcgcggt taagtcggtc atattgttca ccattgcaaa tggtccgtgg tgactgtcgg
    15241 cgcagcaagc ttcgcgaaat agatttagga ataagcgaag aaaaatccct ttgccgacag
    15301 gcgcaaatta aatctcgtca ggtgtacgca ggttggttac ggcaatgcgt tcaccgcgct
    15361 tgcgttcgcg ctctgattgc atattggcgc gataaacgcc ctgatcgcca accacccagg
    15421 agagcggacg acgttctttg ccgatagatt cctgcaacag catcagtgcc tgcatgtacg
    15481 cttcaggacg cggcgggcag cccgggatat acacatcaac cgggatgaat ttatcgacgc
    15541 cctgcacaac ggaataaata tcgtacatac caccagagtt ggcacaggca cccattgaga
    15601 taacccattt tggttccagc atctggtcat acagacgctg aataaccggt gccattttgg
    15661 taaagcaggt tcctgcaacc accatcaggt cagcctgacg cggcgaagca cgcaatactt
    15721 ctgcgccaaa acgcgccacg tcatgcaccg cggtaaacga agtcaccatc tcaacgtaac
    15781 agcaggaaag accgaagtta tacggccaaa ttgagttttt acgaccccag ttaaccatgt
    15841 cattgagctt gcccataaac acgtttttgt taacttcttg ctccagaggg tcggttacga
    15901 tctcctgctt ttgcaggggg taacggtcgt tctcaccgtt gggatctatg cgggtgagcg
    15961 tataatccat cttaatgcct cgcggttagc gttgacgatt agcgatactg ttcgtttccg
    16021 ggttcatacg ctcgcggcgt gaacgcgcgg gcgtccagtc cagcgcgcca atacgcacca
    16081 gataaaccag acctgccagt aacacaaaaa taaaaattgc agcttccaca aagcctaccc
    16141 agccgctttc gcggatagag gttgaccatg cgaacagata cagcgcttca acgtcgaaga
    16201 taacgaagaa catggccacc agataaaact tggcggacag gcgtaagcgg gcggagccga
    16261 ccgagtcgat accggattca aacggcacgt ttttcgacct cgcgcgtgcg cgaccgccta
    16321 aaaaccaacc gcctaccagc atcaggcaac ataggccaat ggcaacgata agaaagatag
    16381 cgaatgccca gtgatgagcg atgacttcag tggatgttga catactcatt gcttactcat
    16441 caaaagtagc gccagattca ctgctcttca cggcagatgg acgccacatc gattcatggg
    16501 gaggaataaa aaaaacctta caatcactgt agaaattctt ttatacagct aattgatgtg
    16561 gtcttttact cctttctata accttttgtc aactttaaca aaagtttctt cacattagtt
    16621 tacataatat caacaccatt agcatttaat gccctttcac cccagatcct tgacgactcc
    16681 aggataatta gatgttgttg aatcgtgtcc gttgtgaagc aatggaaaaa atacgggtct
    16741 attttgacag gaatttgtgt cgattcctcc ccccaaaaga gagtattttc ttgatctgtg
    16801 acacgctttt gtcattccat aacaaaaacg cagcaacaaa tttacgtatt ttttaacatc
    16861 attgtagcag gtgatttttt tcaggcgatt atttgtgcgt acggggcgtg aatctctggt
    16921 gattgaaaaa tgaacagttt tgtacgttct gcacgatgtg aagaaggttc tttactaaaa
    16981 aaaagccgct ggggtttaaa acacccccag cggctcgttt tttacactat tgtctcagga
    17041 attatctatc gtccgtcgat tactcgatat ccctttcaat caacaatgaa tcatcccctt
    17101 ccggagcaga tattgggctg tattgccacg gattatggta gctctccatc gcctgataga
    17161 tcacctgggc cagctcatta ttactggacg ggtcatagca cagcaaatat tcggtatcag
    17221 gtagcggcgg taagccatct actccactca gcacacgcaa gtccggactc atcatctcga
    17281 ccggccttgc cgtcacgcca agaccggctt tcactgccgc acgaacggcc ggaagcgtcg
    17341 aggcgacata agccagtcgc cagggaatat ctgctttatt aagcgtcgcc agcaccatat
    17401 cgcgaaacgg acttggatca tccagcaata caagagggat cggctcacct ttttgcaata
    17461 cgtattccgc tgcgcagtac cagtgtgttg gcgaggtacg caggttcaat gccttaaacg
    17521 ctgaaggacg atgggtggtt accatcaaat ccacttcctg ggattccagc atttctgcca
    17581 tataggcatt acgtttaacg cggacatcca gcgctaattt cggataaacc gaactcacgc
    17641 gatttaacag gaaaggtaag atcgtatcgg cagattcatc tgaagcaccg atagttaaca
    17701 cgccctgaag attactgaac attaatgatg agcaggcctc atcattaaaa cgcaggattt
    17761 tcctggcgta accaagaagt tgaatgccat gttcagttaa cagtttgttg cgaccgtgac
    17821 gagcgaacag ttctttccca acgagttgtt ccagacgctg catttgctga cttacggcgg
    17881 actgagtacg acacacagcg gcagctgcgg cagcaaaagt gttcagatcg gcaacagcaa
    17941 caaatgttct cagcagatcg aggtcgaggt taattatcgg acgatttgca cttatcatat
    18001 attatcactt actggcggct catactgagc tggttaatgc tgtgcacaca caaacaagca
    18061 attccatttg taatgtgcct ccctggcagt ttcatccgga acccggacga aagtaaaaat
    18121 gcatatgagt tgcactaaaa aagcgactca cattgttccg ttataatgcc tgaagtagat
    18181 cacagaatat atcttcaggg atcgcatatc tattaagtta ctcactcttt tctatttatg
    18241 acatgcgcgt gtttgtataa atgtaaatgt gagtccttgt tccactctcg tgcagcatcg
    18301 ctggtcatac gcgaacacgt acaaacagca atggtgaggc ggcatcaaga gcagggatcc
    18361 gtcatttatg cgagcatttt accccaaaaa ccttttattt ataagggtca ttgcgaatta
    18421 tctgatgcaa agttatgtta tgttaggcaa agtaatcttc tattattaat aagcacatca
    18481 aaaccttttt gaatattaaa taataattaa ttagcatcat cctcattcat taattccgct
    18541 taacaatagt ttcacacctt gcccccattg cgacacctcg gaaatcatca cgctgttaaa
    18601 ctctccgtaa tgcggaaata tcatcatcgc gtttcattag tgaattctta aatgaggcat
    18661 tttcacacaa ttatcttaca gataaaaaaa ccagacttac aattaagaat cagaacaatt
    18721 cactatataa cattgcatgt aaagcatatg cacctcatta ttttgtcatt attaagttat
    18781 taacagcaca atcgagcctt cccctctggc aaaatcttat tctgcagacc ttcaaaacac
    18841 cgtcctgggg gagtacattg ttctaagctg acttccacgg cagggagtgg cgataacagc
    18901 aaaaaaggtc aagattcatg tcccccattg aaaaatccag caaattagag aatgtctgtt
    18961 atgacatccg tggtccggtg ctgaaagaag caaaacgcct ggaagaagaa ggtaacaagg
    19021 tactgaaact gaacatcggc aacccagccc cgttcggttt tgacgcgcca gatgaaatcc
    19081 tcgttgacgt gatacgcaac ctgcctaccg ctcaagggta ttgcgattcc aaaggtcttt
    19141 actccgcgcg taaagccatc atgcagcact atcaggctcg tggcatgcgt gatgttaccg
    19201 tggaagatat ttacatcggc aatggtgtat cggagcttat cgttcaggca atgcaggcat
    19261 tgctgaacag cggggacgaa atgttggttc ctgcaccaga ttacccacta tggaccgcgg
    19321 cggtttcgct ttccagcggt aaagcggtgc attatctttg cgatgaatcc tctgactggt
    19381 tcccggacct cgatgatatt cgcgctaaaa ttacgcctcg tacgcgtggg atcgttatta
    19441 tcaacccaaa taacccaacc ggcgcggtat attccaaaga gcttttaatg gagattgtgg
    19501 agattgcacg tcagcataat ctcattatct tcgccgatga aatttatgac aaaattctct
    19561 acgacgacgc tgagcatcac tcaattgcgc cgctggcacc tgacctgctg accattacct
    19621 ttaatggact gtcgaaaacg taccgcgttg caggcttccg tcaggggtgg atggtgttga
    19681 acgggccgaa aaaacacgcc aaaggctaca tcgaaggtct ggaaatgctg gcttcaatgc
    19741 gcctgtgtgc taacgttcct gcgcaacacg ccattcagac cgcgctgggt ggttatcaga
    19801 gcatcagtga atttattacc cctggcggtc gtctttatga gcagcgtaac cgcgcgtggg
    19861 aattgatcaa cgatattccg ggcgtttcct gcgtgaaacc ttgtggtgcg ctgtacatgt
    19921 tcccgaaaat cgacgccaaa cgctttaaca ttcacgacga tcagaaaatg gtgctggatt
    19981 tcctgttgca ggaaaaagtt ctgttggtgc aagggacggc attcaactgg ccgtggccgg
    20041 atcacttccg cattgtcacg ctaccgcgtg tcgatgatat cgagctgtcg ttgagcaagt
    20101 tcgcgcgttt cctttctggt tatcatcagc tgtaatctta atttcactgc cggagattgc
    20161 atccggcagt gttatcccgc cacaatgacc tgatgatgtc atcatacgta aggtcactat
    20221 gaaacagagc catttttttg cccatctctc ccgcctgaaa ctcattaacc gctggccgct
    20281 catgcgcaac gtgcggacgg aaaatgtgtc cgaacacagt ttgcaggtag cgatggtcgc
    20341 ccatgcgctg gcagctatca aaaatcggaa atttggcggt aatgtcaacg ccgaacgtat
    20401 cgctttactg gcgatgtacc acgatgcctc agaagtgctc accggcgatc tccctactcc
    20461 ggtgaaatac ttcaattcgc aaatcgctca ggagtacaag gctattgaaa aaatcgctca
    20521 gcaaaaactg gtcgatatgg ttccggaaga gctgcgggat atctttgcgc cgttaattga
    20581 cgagcatgca tatagcgatg aagaaaaatc gctggtgaaa caggcagatg cactgtgtgc
    20641 atatctgaaa tgtctggaag aactcgcggc gggaaataat gaattcttgc tggcaaaaac
    20701 gcgactggaa gcgacgcttg aagcgcgccg cagccaggag atggactact tcatggaagt
    20761 atttgttccc agcttccatc tttcgctcga tgagattagc caggattcac cgctgtaagc
    20821 agctggagtc tgcgtcgcat caggcaataa gcgccggatg cgacatcagg ctcttgtcaa
    20881 aacggaaaca gcatcgggat catcaccaca caaaccgcca tcacgataat ggtgaacggt
    20941 acccccagct tcacaaagtc actgaagctg taatttcccg gacctaaaac caatgtgtta
    21001 acaggtgaag aaaccggtgt cataaaggcg gcggatgctg ccatcgccac gaccatcgcg
    21061 aatggataag gcgacacccc catcgttttg gcagcagcca gcgcaatcgg agccatcaac
    21121 accgccgtcg cggtattaga gataaatagc ccaataaccg ccgacaagac aaacaaacaa
    21181 cccagcatca tatgtggccc gtaaccaccg ccaatgtcca tcagcccttt caccgccagc
    21241 gcgacacctc ccgttttctg taatgccaca gcaaacggca tcatcccaac gatcaaaata
    21301 atgctcggcc agtgaatgga tttataggcg ctttcagcat ctatacagcg gaatttcccc
    21361 atcagcaggc aggcgatgat agcggcaaca ggattaggaa tttcatctgt cagcattaac
    21421 gccaccatta gcaccagaca gaaaatggca tggggtgcct ggctgtgcgc gggcgatgct
    21481 tcactcacct cttccggtaa gttcagcgct acgaagtcgc ggccctgttt ggccagcata
    21541 ccgatcagtt tccagttacc cacaaccagg atgatatcgc ccagcagcag aggctcatcc
    21601 gccagcgaac cttccagcgc cacgccattg cgctttagcc ccaccacatt cagtccgtag
    21661 cgggtacgaa aaccaatttc gcgcaccgat ttaccaatca gttctgactc aggaattaat
    21721 gaaatctctg ccatgcccac atcaagggcc tggtcagaaa aatactcgcc gcgcagtacc
    21781 atcggctcca gcaattgctc actacaaaat tgccggagat cgacatcagc cgcagacata
    21841 tcaataagca aaacgtcacg cgcgcgaaat tcagaaaccc cattaacgtt cacgataacg
    21901 cgacgaaaac gccgccagcg ttcaacaccg atgacgttag cgccataacg ctcacgtaat
    21961 ttgagatcat ccagccgttg accaatcatt ggcgatccgg ggcgaatagc cagacgtcgc
    22021 gcacgcccgg tcagtcgata ttcacggata agatcgcgaa aggttcgacg cgtccagcct
    22081 tcgcgctgcg gggtctgggt atcccctttc agcatgaaac gcatcactaa catatacaaa
    22141 atacccagca ccagcacgac caggccaata ggtgttacgc taaagaaact gaagccgtga
    22201 tagccttcac gcagcaattc actgttgact accaggttcg gcggcgtcgc caccagcgtc
    22261 atcatgccgc taatcagccc ggcaaaactt aacggcatca tcagacgtga cggcgaagtt
    22321 tgcatacgca tggcaacgct taacaccacg ggaataaaga tagcgacaac gccggttgaa
    22381 ctcataaacg cgccaagccc cgcgacggtc agcatcaaca aaaccaacat tttgatttca
    22441 ctgttgcccg caactttgac cagccatgtt cccattacgg tggcaacacc ggtacggacc
    22501 aaaccatcgc caataataaa caaggcggca atcaggacaa cgttaggatc agaaaagcca
    22561 gaaaatactt ctgggactgt cagcgttccg cttaatgcaa acgcgacaat aacaaacaaa
    22621 gcgaccgcat ccatacgcac tctgcccgtc gcaaacaaga cgatggcaac ggccagtaat
    22681 gaaagaaccc aaatcaattc accgttcaca acttatcctt gttaattgag ggggatgact
    22741 tgattctgcc ataaaaaagc cccgacgaga cggggctaaa tcatgatcag gtgtttcact
    22801 gaataataac atcgccattt ggctgtttgg tcacagtaat ttgctccaga ctgtggagga
    22861 ccaaatcgac ctcattcagg cgcggggtat ctgccggagc gttaaccgca atgacatggc
    22921 aacccgccgc caggccagaa agcacgccag cgggagcatc ttccaccacc acacactcct
    22981 gcggcgcaag ccccagcagc tgcgcgccta acagatacgc atcaggttct ggttttccgc
    23041 gcttcactcg ctcagcggtt acaaacacct ctggtgcggg aagccccgct attttatggc
    23101 gcgctcgcgc taccggcatg gagccagaag tcacaatggc ccacggaata cctgctttat
    23161 tcaaatgatt gagtaaggcg attgcccccg gaagcgcggt aataccttcg gtttccgtgg
    23221 cctcgatttg ctccaaacgc gtaaactcgg cggcaatatc agcctcggat ttgcccgcca
    23281 taaaatggcg cagagaggtg atcgcctgtt taccgtgaat gaaagccagc acctcttccg
    23341 gcgctaaccc atgacgtctg gcccagttgc tccacgcccg ttctaccgca ggcagggaat
    23401 ccaccagcgt tccatcaaga tcaaacagaa aacctttgca ccgcacgcgg gcctcctcag
    23461 gcgttgataa tttgattaat ttcgttggcg ctcaaatggt actgacgcgg gcaggcatgc
    23521 cacacattaa gcatgcgctg atatttttcc cacattggcg tctgggcgtt aaaaccgtga
    23581 gttccggcgt caaaatgggt atagcgccct tccacattaa ccataaagcg gacataaccg
    23641 aggtaacgtg cttcagtggc ggcgtcaaag ccgaggaagg tgacacgacg ttcatcgatg
    23701 gattgctggt cctgcaaatt agaccaggaa acatgcaacg catgatacat ctccataatg
    23761 tcgatgatag tgcggcaggt ttcttctttc agctcgccaa actcgcgatc cagttcacgc
    23821 atctgtaatc cataaccccg ctcaatgatt gtttgcaggc gacggtaacg ttcagcattt
    23881 gccggatcga gcatagtcat catcttgtac tggttagaca aaataagacg ttgcgcgttg
    23941 gtcatttcca ttgttgactc ctgtatcact ctactacggt gaaaaaaaag aaggctgagt
    24001 atgccttctt ttatatgcgt aatcaggggt caattacaaa tcatcaagga aagttttatc
    24061 cagttgtttg aaggcacgct taagcgtgtc agctaatgcc tggtaatcag gcttgccttc
    24121 aacgggtgcc aacacctgtc cagactcctg caatttaccg cgaacttcat aaaaccagtt
    24181 gaggattgca gggggtaatg gcgtgacaga acgcttgccc agccaccaca atccctgcat
    24241 gggtaaactt aaggcgaaca gggcagtggc aactgccggc ccaagctgac cgcccagggc
    24301 aatctgccag cagagagtaa atacggcgat cggcggcata aaacggatcg cataacgcgt
    24361 catcttgata acgcgatttt cgacaaagac cggggcaagg cgtttttcca gcggccacgt
    24421 ctttgagtaa tgctgtcccc ggcgaaacaa gctaaaaaaa ttaacagaac gattatccgg
    24481 cgttgacatg cttcacctca acttcacata taaagattca aaaatttgtg caaattcaca
    24541 actcagcggg acaacgttca aaacattttg tcttccatac ccactatcag gtatccttta
    24601 gcagcctgaa ggcctaagta gtacatattc attgagtcgt caaattcata tacattatgc
    24661 cattggctga aaattacgca aaatggcata gactcaagat atttcttcca tcatgcaaaa
    24721 aaaatttgca gtgcatgatg ttaatcataa atgtcggtgt catcatgcgc tacgctctat
    24781 ggctccctga cgttttttta gccacgtatc aattataggt acttccatgt cgagtaagtt
    24841 agtactggtt ctgaactgcg gtagttcttc actgaaattt gccatcatcg atgcagtaaa
    24901 tggtgaagag tacctttctg gtttagccga atgtttccac ctgcccgaag cacgtatcaa
    24961 atggaaaatg gacggcaata aacaggaagc ggctttaggt gcaggcgccg ctcacagcga
    25021 agcgctcaac tttatcgtta atactattct ggcacaaaaa ccagaactgt ctgcgcagct
    25081 gactgctatc ggtcaccgta tcgtacacgg cggcgaaaag tataccagct ccgtagtgat
    25141 cgatgagtct gttattcagg gtatcaaaga tgcagcttct tttgcaccgc tgcacaaccc
    25201 ggctcacctg atcggtatcg aagaagctct gaaatctttc ccacagctga aagacaaaaa
    25261 cgttgctgta tttgacaccg cgttccacca gactatgccg gaagagtctt acctctacgc
    25321 cctgccgtac aacctgtaca aagagcacgg catccgtcgt tacggcgcgc acggcaccag
    25381 ccacttctat gtaacccagg aagcggcaaa aatgctgaac aaaccggtag aagaactgaa
    25441 catcatcacc tgccacctgg gcaacggtgg ttccgtttct gctatccgca acggtaaatg
    25501 cgttgacacc tctatgggcc tgaccccgct ggaaggtctg gtcatgggta cccgttctgg
    25561 tgatatcgat ccggcgatca tcttccacct gcacgacacc ctgggcatga gcgttgacgc
    25621 aatcaacaaa ctgctgacca aagagtctgg cctgctgggt ctgaccgaag tgaccagcga
    25681 ctgccgctat gttgaagaca actacgcgac gaaagaagac gcgaagcgcg caatggacgt
    25741 ttactgccac cgcctggcga aatacatcgg tgcctacact gcgctgatgg atggtcgtct
    25801 ggacgctgtt gtattcaccg gtggtatcgg tgaaaatgcc gcgatggtcc gtgaactgtc
    25861 tctgggcaaa ctgggcgtgc tgggctttga agttgatcat gaacgcaatc tggctgcacg
    25921 tttcggcaaa tctggtttca tcaacaaaga aggtacccgt cctgcggtgg ttatcccaac
    25981 caacgaagaa ctggttatcg cgcaagacgc gagccgcctg actgcctgat ttcacaccgc
    26041 cagctcagct ggcggtgctg ttttgtaacc cgccaaatcg gcggtaacga aagaggataa
    26101 accgtgtccc gtattattat gctgatccct accggaacca gcgtcggtct gaccagcgtc
    26161 agccttggcg tgatccgtgc aatggaacgc aaaggcgttc gtctgagcgt tttcaaacct
    26221 atcgctcagc cgcgtaccgg tggcgatgcg cccgatcaga ctacgactat cgtgcgtgcg
    26281 aactcttcca ccacgacggc cgctgaaccg ctgaaaatga gctacgttga aggtctgctt
    26341 tccagcaatc agaaagatgt gctgatggaa gagatcgtcg caaactacca cgctaacacc
    26401 aaagacgctg aagtcgttct ggttgaaggt ctggtcccga cacgtaagca ccaatttgcc
    26461 cagtctctga actacgaaat cgctaaaacg ctgaatgcgg aaatcgtctt cgttatgtct
    26521 cagggcactg acaccccgga acagctgaaa gagcgtatcg aactgacccg caacagcttc
    26581 ggcggtgcca aaaacaccaa catcaccggc gttatcgtta acaaactgaa cgcaccggtt
    26641 gatgaacagg gtcgtactcg cccggatctg tccgagattt tcgacgactc ttccaaagct
    26701 aaagtaaaca atgttgatcc ggcgaagctg caagaatcca gcccgctgcc ggttctcggc
    26761 gctgtgccgt ggagctttga cctgatcgcg actcgtgcga tcgatatggc tcgccacctg
    26821 aatgcgacca tcatcaacga aggcgacatc aatactcgcc gcgttaaatc cgtcactttc
    26881 tgcgcacgca gcattccgca catgctggag cacttccgtg ccggttctct gctggtgact
    26941 tccgcagacc gtcctgacgt gctggttgcc gcttgcctgg ctgccatgaa cggcgtagaa
    27001 atcggtgccc tgctgctgac tggcggctac gaaatggacg cgcgcatttc taaactgtgc
    27061 gaacgtgctt tcgctaccgg cctgccggta tttatggtga acaccaacac ctggcagact
    27121 tctctgagcc tgcagagctt caacctggaa gttccagttg acgatcacga gcgtatcgag
    27181 aaagttcagg aatacgttgc taactacatc aacgctgact ggatcgattc tctgactgcc
    27241 acttctgagc gcagccgtcg tctgtctccg ccagcgttcc gttatcagct gactgaactt
    27301 gcgcgcaaag cgggcaaacg tatcgtactg ccggaaggtg acgaaccgcg taccgttaaa
    27361 gcagccgcta tctgtgctga acgtggtatc gcaacttgcg tactgctggg taatccggca
    27421 gagatcaacc gtgttgcagc gtctcagggt gtagaactgg gtgcaggcat tgaaatcgtt
    27481 gatccagaag tggttcgcga aagctatgtt ggtcgtctgg tcgagctgcg taagaacaaa
    27541 ggcatgaccg aaaccgttgc ccgcgaacag ctggaagaca acgtggtgct cggtacgctg
    27601 atgctggaac aggatgaagt tgatggtctg gtttccggtg ctgttcacac taccgcaaac
    27661 accatccgtc cgccgctgca gctgatcaaa actgcaccgg gcagctccct ggtatcttcc
    27721 gtgttcttca tgctgctgcc ggaacaggtt tacgtttacg gtgactgtgc gatcaacccg
    27781 gatccgaccg cagaacagct ggcagaaatc gcgattcagt ccgctgattc cgctgcagcc
    27841 ttcggtatcg aaccgcgcgt tgctatgctc tcctactcta ccggtacttc tggtgcaggt
    27901 agcgacgtag aaaaagttcg cgaagcaact cgtctggcgc aggaaaaacg tcctgacctg
    27961 atgatcgacg gtccgctgca gtacgacgct gcggtaatgg ctgacgttgc gaaatccaaa
    28021 gcgccgaact ctccggttgc aggtcgcgct accgtgttca tcttcccgga tctgaacacc
    28081 ggtaacacca cctacaaagc ggtacagcgt tctgccgacc tgatctccat cgggccgatg
    28141 ctgcagggta tgcgcaagcc ggttaacgac ctgtcccgtg gcgcactggt tgacgatatc
    28201 gtctacacca tcgcgctgac tgcgattcag tctgcacagc agcagtaacc tcgtcatcat
    28261 ccgcagcttt gcgctgcgga tatctgaacc ggaaatagtc actatttccg gttttttatt
    28321 ctcttaattt gcattaatcc tttctgatta tcttgcttaa ctgcgctgca tcaatgaatt
    28381 gcgccatctc actttgcata cttaccactt tgttttgtgc aagggaatat ttgcgctatg
    28441 tccgcaatca ctgaatccaa accaacaaga agatgggcaa tgcccgatac gttggtgatt
    28501 atcttttttg ttgctatttt aaccagcctt gccacctggg tagttccggt ggggatgttt
    28561 gacagtcagg aagtgcagta tcaggttgat ggtcaaacaa aaacacgcaa agtcgtagat
    28621 ccacactcat ttcgccttct gactaacgaa gcaggcgaac ctgagtatca ccgcgtacag
    28681 ctgttcacga cgggcgatga acgcccgggc ctgatgaact tcccgtttga agggttaacc
    28741 tcaggatcga aatacgggac agccgttggc atcatcatgt ttatgctggt gattggcggc
    28801 gcgtttggca ttgtgatgcg tacaggaacc attgataacg gtatcctggc gcttattcgc
    28861 catacccgtg ggaatgaaat tctctttatt cctgcgctgt ttattctgtt ttcacttggc
    28921 ggcgcggtat ttggtatggg ggaagaggcc gtcgcctttg ccattatcat cgcaccgcta
    28981 atggtccggc tgggctatga cagtattacc accgtcctgg tgacctatat tgccacgcaa
    29041 atcggttttg ccagttcgtg gatgaacccg ttttgtgtgg tcgttgctca ggggattgcc
    29101 ggcgttccgg tgctttctgg ctccgggttg cgcatcgtgg tgtgggttat cgccactctg
    29161 attggcctga tctttaccat ggtgtacgcc tcacgagtga aaaagaatcc tcttctgtca
    29221 cgcgtgcatg agtccgaccg cttctttcgt gaaaagcagg cggatgttga acaacgtccg
    29281 tttacctttg gtgactggct ggtattgatt gtcctgaccg ccgtaatggt ctgggtgatt
    29341 tggggcgtga tcgttaatgc ctggtttatt ccagaaattg ccagccagtt cttcaccatg
    29401 ggtctggtga ttggcatcat cggcgttgtt ttccgcctta acggtatgac ggttaatacc
    29461 atggcttcat ccttcaccga aggggcgcga atgatgatcg cccctgccct gctggtgggt
    29521 ttcgccaaag ggattttgct gctggtcggt aatggtgaag cgggtgatgc cagcgtgtta
    29581 aatactatcc tcaacagcat tgccaatgcc attagcggtc tggacaacgc ggtcgcggcc
    29641 tggtttatgt tgctcttcca ggcggtattt aatttcttcg tgacgtccgg ttctggtcag
    29701 gcggcgttaa ccatgccgtt actggcaccg cttggcgatc tggtcggtgt taaccgtcag
    29761 gttaccgtgc tggctttcca gtttggtgat ggcttcagtc acatcattta cccgacctca
    29821 gcttcgttaa tggcgacgct cggtgtttgc cgggtggact tccgtaactg gctgaaggtg
    29881 ggcgcgaccc tgcttggact gctgtttatt atgtccagcg tcgtggtgat cggcgctcag
    29941 ttgatgggct accactaaaa atgttaagag ccgcaattgc ggctcttttt tcattctgcc
    30001 gtttcagtct ctacggcttc atttttggca ttgcgcttca tccacaacgc tagcgctttc
    30061 agcgagtctg gagtgaactc atcgcagcgt gcggtgattt cttccggcgt cagccagcaa
    30121 acttcactga cttcatcttc ctgtagggcg aagggaccgt gagagacgca gctgaacaat
    30181 gcgccccaga cacggcaatt tttatcttcg aaatagaact gcccgtgctc ggcaaagggg
    30241 acaccggcaa tgcccaactc ttcttccgct tcgcgacgcg cggattccag cagttgctca
    30301 tcggcctgga ctacaccgcc agcggtcgca tctaacatgc cgggtaaaaa gtcttttgtc
    30361 tcggtacgac gctggaccag aattttgccc atgccatcat gcacgacgat gtaagttgca
    30421 cgatgacgca gacactgtgc ccgcatttgc tcccggctgg cttgtgcaat gacttcgttc
    30481 tcttcattga caatatccac ccattcagta cttgccaaac gacgctgttc cattatcagg
    30541 aaaccttctt tttctggcgc tcttacggcg cattttgagt tgtgggtaaa ttacggatta
    30601 atcgcgacct gcgcaatgat actttgatca ttgagtgcga ttacactaag tacgtcatta
    30661 tccagcatgc cataactcgc cggattaccg cctttcggaa tactcaccga gccggggttg
    30721 aagtggaaaa tctccccccg ctgttctgct actggcagat gggtatgacc gtaaaccagc
    30781 acatcgttct ggtttaaagc aggcagattt tccgggccaa aaagatgacc atgcgtcaaa
    30841 aacagacgtt gtttttccag taatacctgt tgccacggcg cggttatcgg gaaatgcagc
    30901 agcatttgat ccacttcgct gtcgcagttg ccgcgcacag cgataacctt atgtgccact
    30961 tcattaagcc gttcagcgac tttggctggc gcgtaaccct ccggtaaagc attacgcggg
    31021 ccgtgattca acacgtcgcc aaggatcacc agccactggg caccgctttg ggcaaacaac
    31081 tccagaacac gttccgtcgc cggtaacgac ccatgaatgt ccgatgcaaa catcagtttc
    31141 atcactcact cctcgtcgaa aaaacgtaag ctatgatact ggattctgtc gttgctatca
    31201 gccagattgc ttcgctgaaa gtgcaataaa acgctggaca gacgctcgct gccactggaa
    31261 tgttgcataa tccaccaggc attctggcac ctcatcgcca tgtagtacca ggcggttaat
    31321 cattagcgcc agatcagtat cagcaatgca ccattcacca aataaattcg gctgacccag
    31381 tactaacaaa tgttctgcca tcgcgaacag tttctctgca ctggcttttc cctcggccgt
    31441 tagtggcgct tttttcgccc ccgcaaagac aacatctgtc ggacgctctt cgcggatggg
    31501 catcagatcg ctgcgcaacc aggcctgaat ctgtcgtgca cgcgcacgat tttctaaatc
    31561 aagcggataa atacgttccc aggtcggtgg cgcaaatcga tcttccagat actccgcaat
    31621 ggcagaagat tcactcaact caaaatcatc gatttgtaat aacggcacac ggcgtgtctg
    31681 accgtaacct tgccacgtcg gttgcaaatg ttcaccgctg tcgaggtcga tggtcttgat
    31741 atgaaacaac aggccttttt cctgcaacgc cacccaagcg gataacacat aaggggagaa
    31801 aaaatgggca tctgaccaaa gcgtgatagc gggtttactc attatgtcct cggcaataaa
    31861 gggggctggc tatcaaacta tagagccttt ttgtcactgt cacgtgacga atactcacgt
    31921 aagtcgccgc gctttctata attgtggtgt tcagatgatt agacaaccgg agctccaatg
    31981 atcgatctct atttcgcccc gacacccaat ggccacaaaa ttacgctgtt tctcgaagaa
    32041 gcagggctgg attatcgctt gataaaggta gacctgggga aaggcggtca gtttcgcccg
    32101 gaatttttgc tcatttcgcc taacaacaaa attccggcaa ttgttgatca ttctccagcc
    32161 gatggcggcg aaccgctaag cctctttgaa tctggtgcca ttttgttgta tctggctgag
    32221 aaaacggggc tctttttgag tcatgaaacg cgtgaacgcg ccgccacatt acagtggtta
    32281 ttctggcagg taggcggact ggggccgatg cttgggcaaa atcatcattt taatcacgca
    32341 gccccccaaa ccattcctta cgctattgaa cgttatcagg ttgaaactca gcgtctgtac
    32401 catgtactga acaagcggct ggaaaactcg ccctggctgg gaggcgagaa ctacagcatt
    32461 gcggatattg cctgctggcc gtgggttaat gcctggactc gccagcgaat agacctcgct
    32521 atgtatccgg cagtcaagaa ctggcatgag cggatccgtt cgcgccctgc caccgggcag
    32581 gcactgctaa aagcacaact cggtgatgag cgttcggata gttaacagaa acaggttctc
    32641 gtgtattatt tcatcctaag taaaacaacg gagaacctgc aatggcacaa cctgccgcta
    32701 ttattcgtat aaagaacctt cgtttacgta catttatcgg aattaaggaa gaagaaatta
    32761 acaaccgtca ggatattgtt atcaatgtga cgatccacta ccccgccgat aaagcgcgca
    32821 ctagcgaaga tatcaacgat gcgctgaatt atcgcaccgt aacgaaaaac attattcagc
    32881 atgtagagaa taaccgtttc tctttgctgg aaaaattaac tcaggatgtg ctcgatatcg
    32941 cacgtgaaca tcactgggtg acgtatgctg aagtggagat cgataaactg cacgcgctgc
    33001 gctacgccga ttcggtatcc atgaccttaa gctggcagcg ttaaccgcca tatcgggagg
    33061 ctgcatgaac atagtgatca ccggagggac gggattaatt ggtcgccatt tgattccacg
    33121 tttgctggag ctgggccatc aaatcacggt aatgacgcgt aacccgcaga aagccagttc
    33181 cgttctcggc cctcgggtga cactatggca agggcttgcc gatcaaagca acctcaacgg
    33241 cattgatgcg gtaatcaacc tggccggaga accgattgct gataaacgtt ggactcacga
    33301 gcaaaaagag cgtctctgcc aaagccgctg gaatatcacg caaaaactgg tcgatttgat
    33361 taatgccagc gacacgccac cgtcggtact catttccggc tcggcaacgg gctattatgg
    33421 cgacttaggt gaagtggtgg ttaccgaaga ggaaccgccg cataacaaat ttacccataa
    33481 actctgcgcc cgctgggaag aaattgcctg tcgggcgcaa agtgacaaaa cgcgagtgtg
    33541 cctgctgcgt accggcgtag tgctggcacc ggatggcggt attctcggta aaatgctgcc
    33601 gccgtttcgt cttggcctgg gcgggccgat tggttccggt cggcagtatc tggcctggat
    33661 tcatatcgat gatatggtca acggcattct ctggctgctg gataacgagc tgcgcgggcc
    33721 atttaatatg gtttcgccct acccgatacg caatgaacaa tttgcccatg cgctcggtca
    33781 tgcgctgcat cgcccggcta ttttgcgcgt ccccgcaacc gccattcggc tgttaatggg
    33841 cgaatcttca gtactggtat taggtggaca acgcgcgctg cctaaacggc tggaagaagc
    33901 gggttttgcg tttcgctggt acgatttaga agaggcgctg gcggatgtcg ttcgctgatg
    33961 tggtttactg caaacatccg ccagttaact cccggtgtta cagggttagt ggctttgcgc
    34021 gataagatcg tctggtgaaa gtcgggtcag cgtcaggatg acttcgtggt caaatccttt
    34081 ttccagcatc tcctgagcaa tacgcagagc ttcttcgtgt ttgccctgca ttgcgccttc
    34141 ttcacgtaat ctgtcagcaa tggtcatcag tttctccttt tcttgtggtg cgcgttctgt
    34201 tatctcacca ataaatgcac ggaaacgctg ggcatctccc gtttgtaata cataattaaa
    34261 cagggctttt agctgtctgt cattagtgtt ccctgtaact agcagcgaaa caatttggtc
    34321 gactaatccc aacagatcgc gctgacgaat atgtttctga attaactcca acagcgccat
    34381 tttacgatgt tgcataatct cgtcatccgg caccacggta atatccacca acggaaaagc
    34441 cgatgaatat attttacggg caatcgcagg ctcggcaaat tcatcaagcc agcagagtga
    34501 ataaggataa ggacttctgc aaccatgata aaacagcatc gggatcacca atggaagctc
    34561 tttatagccc acatcaagat ggttttgcat tgccgcaatg gaataacgca tcatgcgaaa
    34621 agccattaat tcttccggct tactttggtg ctctatcact acataaagat aacccactcc
    34681 ctcctgcgtt ttcacagacc acaagaggtc ggaataatat tgccgcaggt cttcatcaat
    34741 aaaactgttt ggttccagtt taagcgtcgt taaatcacac agtttgcgca gcggcgcggg
    34801 aagatgaata tcaataaaat cccgcgcggt gtctggatgg cgtaaaaaag atttaaatac
    34861 cgcatcatgc ggcgtggaag ttgtcgatat tgtcatggcg ttccgtcacc cctcaaattt
    34921 aagatgacgc gacaataacc acgattaaca cccaatgcag caggcagttt ttatctttgg
    34981 acagcgaatt cagaagtatt tttagcggac tgcaactttt acatttcagc ggaagctgac
    35041 tcgcaaattc cccttgaaaa tactccggca gcgttagcca ccggagaggc aattatttca
    35101 gcgaaccttt aaggaactgt tgcagacgag ggctttgcgg gttgccaaat aactgctccg
    35161 gcgcgccttc ttcctctatt ttcccctgat ggaggaaaat gacgtgagta gatacatggc
    35221 gagcaaagcc catttcgtgg gtcaccacca ccatcgtttt tccctcttcc gccagctgct
    35281 gcataatgcg cagcacttca cccaccagct ctgggtcaag ggccgatgtc ggttcatcaa
    35341 acaacagaac ttccggttcc attgccagcg cccgcgctat ggaaacacgc tgttgctgac
    35401 caccggacag gtgcaccggg tatttcccct gcgcacgttc gtctatcccg acttttgcca
    35461 gatacttcac cgcccgctcg cgcgcttcct gcttgctcag gcccaacacc tgaatcggcg
    35521 cttccatgac gttttccagc accgtcatat ggctccagag attgaagtgc tggaatacca
    35581 tcgtcaggcg tgtgcgcagt aagcgcagtt gatttttatc ggcgactttg agttgaccgt
    35641 ctttgtcgcg caccagattg atcgtctggc cattgaccac gatcgaccct tcactcggtt
    35701 tttcgaggaa gttaatgcag cgcagaaagg tacttttccc cgatcccgac gatccgatga
    35761 tgcttattac atctccggca ttcgcttgca gtgacacccc tttcagcact tcgtgttcgc
    35821 catagcgttt gtgcaaatcg ataacgttta atttattctc ggacatcgta ttctcagtgc
    35881 gttgaagaag gtttcacatg ctgcaaccag cgtttttccg ctctgcgaaa gaggctgatc
    35941 aggacataag agatgattaa atagagcacc gcggcaatgc cgaaggcggt aaaaggttga
    36001 tacgtggcgg cgttaatatc gcgggctatt ttcagcagat ccggcaccgt ggcagtaaat
    36061 gccaacgcag tagagtgcag catcaggatc acttcgttgc tgtatgccgg taacgcaata
    36121 cgcagcgcag aaggcaaaat aatgcagcga tacattttaa aagtcgagaa gccataggct
    36181 ctggcggctt caatttcccc atgcggcacc gaacggattg ccccagcaaa aatctcggta
    36241 gtgtaagcgc aggtgttaag cgtcagcgcc agcacggtac agttcaggcc actgcggaaa
    36301 aaggcgttaa gaaactcggt ccccttaaca atctccagcg tatacatgcc ggaatagaac
    36361 accagcaact gaacatacag cggcgtaccg cgaaaaatat aggtaaataa ccagattgga
    36421 aactggatgt atttattact ggagacacga ccaatcgcca gaaacagcgc caggactccg
    36481 cctatcacta ccgacaaaat aagcagccac agagtgatcg ccacaccagt aaagcgataa
    36541 ccgtcggtcc acagcagcgg tttccagtat tcatgtaaga tttcgatcac aggtcagccc
    36601 tcttcacacc cacagagtag cggcgctcaa ggaacagcag cacaccattg gaaacggtgg
    36661 tgaaaaccag gtaaatcacg ccacagacga tagcgaaata gaacggttcc caggtacttt
    36721 tgcctgccag ctgcgtggct ttgaccacat cttccaggcc gagtaacgaa accagtgcgg
    36781 tagatttgag gatcacctgc cagttgttgc caatgcctgg cagggcgtaa cgcatcatcg
    36841 ccggaaacat gatccgccga aacacttgcc cacgagtaaa accgaacgcc ctcgccgcct
    36901 ctatatgtcc tttcggcact gccataaaag caccacggaa cgtttcggta aaataagcac
    36961 cgtagataaa accgagagtg ataataccag cgaccatcgg atcgatatca atctgcccga
    37021 cgcccatcgc ctccgtcacc gtgtttagcg caatctgcaa accgtagaaa atcagcagca
    37081 tcaacactaa gtccggcacg ccacgaatca gcgtggtgta cccttcgaaa atcagccccg
    37141 aaagccgatt ttgcgagagc ttaccgccag cgccaattaa accgatgatt acagcgagca
    37201 ctacagagct gatagccagc tccagcgtga cgagcgcacc ctgtaaaata acacctgaaa
    37261 acccatacaa catgctgcct gtcctgtcgt gtgtggtgaa ttaccgcctc ttgtctccct
    37321 ccggttaccc gaagggagcg ggctttcttt caccgatggc gattaaccac cataaacatc
    37381 aaaatcgaag tactttttcg ctaatttctc gtaagtaccg tcagcgcgca tttcggcaaa
    37441 ggctttgttc agtgcctcgc gcagttcgtt atcttcttta cgcaggccca tgccggtccc
    37501 tacgccaaac agtttttcat ctttaacaga cgggccaccg aatttgtaat ctttaccgac
    37561 aggttgtttg aggaaacctt cgctggcagc gacctcatcc tggaacgcgg catcaatacg
    37621 tccggcagtc aggtcagaat aaatgttgtc ctgcccctga tacgagacga tttcaatgcc
    37681 ttttggtgcc caatgttcat taccgaacgt ctcctgggta gtgccctgca atacgcctac
    37741 ccgtttgcct ttcagcgact cgactgtcgg ctgaatgtca gaatttttcg ccaccaccag
    37801 acgagaatcg gcagcgtaca gtttgtcggt gaaggctatt tcttgctgac gtttttccgt
    37861 aatggaaagc gatgacatga tggcgtcaat cttcttcgct tttaaggacg ggattaacgc
    37921 atccagcgga ttttcgacaa acgtacattg cgtattgatg cgtttgcata attcctttgc
    37981 cagatcgata tcgaagccaa ccagttcgcc ttgtgaattt tttgattcaa atggcgcata
    38041 ggtcgggtcg gtaccgatgc ggatgttttg cggaatcgca gcaaatgccg cagttgcgct
    38101 ggagaaggcc agaaccagag agagcgatag caccagtttt ttcataatgt cctcaactga
    38161 cagtcttttt atagggaata tttacaggtt tgttacactt atcgtgccat aaaattgacc
    38221 atcaaggcaa aatatcccgc cccgttagac gtattatttt tcattaaaaa tgcttaagta
    38281 tgcatttcaa tgcaccacga tagtgcaaca aacctggcgt gcaccatgat gatcattcca
    38341 tcaggtacag cttccctgcg acgtatcagt caccgtagac attaaagtca aaatactttt
    38401 tcgccatctt gtcgtaggtg ccgtcctgac gcagctcgcc aagcgcctta ttgaaggcag
    38461 ccgtcagttc agcatcatct ttacgtagcc ctacaccggt gccatcaccg aagtattttt
    38521 tgtcttttac tgatgagcca gcaaaggcga aatctttacc agcaggttgc ttgaggaatc
    38581 cttcgctggc agcaacttca tcctgcagtg cagcatccag acgtcctgca gccagatcgg
    38641 aatagaccaa atcctggttg gcataggcca ccacatccac acctttgcta cgccaggtct
    38701 cgttagcgta agcttcctgg gttgatccct gcagcacacc aacatgttta cctttcagtg
    38761 agtccagcgt tggctgaatc ggcgaacctt tggcggcaat cagacgggaa tctgcggcgt
    38821 acagcttgtc ggagaaggca atctcctgct gacgtttatc ggtaatggaa agcgacgaaa
    38881 taatggcgtc gatttttttc gctttcagtg aggggatcag cgcgtcaaag tcactggcaa
    38941 cccaggtaca tttcacctgc atccgtttgc acatctcgtt accgagatcg atatcaaagc
    39001 cgacaaagtc acctttggca tctttcgacg agaacggtgc ataggtggta tcggttccga
    39061 tacgtaccgt ctccggcaat gccgcatagc tggaagccgc tgcggaaata ccgactaaca
    39121 aagacagagc gagaatcgac ttcttcatac ataaccctca agtgaaaatg gcttttttat
    39181 gttgtgtatt gtgttgtgtg tttgcaggct tgttcatgca ggtcttatgc catcttgacg
    39241 acaacagtaa cattcaacgt taaatatgtt aataagacgt tgcattattg tcctaaagtt
    39301 gaagatagca ggtatagcgg ttgaatcgca gcgtttcgat tgaatggcag aaacaaaatg
    39361 tcgaggattt gatcgcggtt gcaaaattgc cctgaaacag ggcaacagcg gagttatgcg
    39421 ccctgccagc gggcaaagag atcttcagga agggttatcg caaactggtc aagaacacga
    39481 ttaaccgtct gatttatcac atcatcaagg gattgcgggc gatgataaaa cgccggaacg
    39541 ggaggcataa tcaccgcacc gatttctgct gcctgcgtca ttaaacgcag atggcctaag
    39601 tgcaatggtg tttcacgcac gcagagcacc agtgggcgac gctctttcag caccacatct
    39661 gccgcacggg tcagtaagcc atcagtatag ctatggacaa tgccggacag ggttttgatt
    39721 gaacagggta aaatcaccat ccccagtgtc tggaaagaac cggaagagat gctggcggca
    39781 atatcgcgcg catcatgcgt gacatcagct aatgcctgca cttcgcgcag ggaaaaatcc
    39841 gtttcgaggg ataaggtctg gcgcgccgcc tggctcatta ccagatgcgt ttcgatatct
    39901 gtgacatcgc gcagaacctg taataagcgc acaccataaa tcgcgccgct ggcaccgctg
    39961 atgcccacaa taagtcgttt cataaatagt tgccctgtca gacttgcagg cagactttgc
    40021 aggatttcgg cgggagttgc aagtcagggt gccagaccgg caccctgagc gaaggcatca
    40081 tccttcgtta tgcatttcga gattttccac ttcgttctga cgttgcactg ctttggcgtc
    40141 gtcattacgt aaagtatcga ggaaatcgag gtagccctga tcaacatctt tggtgacgta
    40201 gacgccgttg aacaccgagc attcaaactg ctggatatcc gggttttcag cgcgaacggc
    40261 ttcgatcaga tcgttcagat cctggaaaat caacccgtca gcaccgatga tctggcgaat
    40321 ttcatctact tcgcgaccgt gagcgatcag ttccgtggcg ctcggcatat caataccata
    40381 aacgttcggg aagcgaattt ccggtgccgc agaagcgagg tacactttct tcgctccggc
    40441 ttcgcgtgcc atctcgataa tctgctcaga agtggtgcca cggacgatag agtcgtcgac
    40501 cagcaggacg tttttatcgc ggaactcggc gcggttggcg ttcagtttac ggcgcacgga
    40561 cttacgacgc agctgctgac ccggcatgat gaaggtgcgg ccaacatagc ggtttttaac
    40621 gaagccctgg cggtacggtt tgcccagaat acgagcaatt tccagcgcga tatcacacga
    40681 ggtttccggg atcgggatca ccacgtcgat atccagatct tcccattcgc gggcaatttt
    40741 ctcgcccagc ttcgtgccca tattcacacg cgcgctgtaa acggaaattt tgtcgatgaa
    40801 cgagtccggg cgagcaaagt atacatactc aaacaggcac ggattgctga ccggattgtc
    40861 agcacattga cgggtaaaca actgcccttc ttcagtgatg taaatcgctt cgccaggcgc
    40921 gacgtcacgc aggaaatcaa agcccagtgt atcgagcgca acgctttcgg aagcgaccat
    40981 atattctgtg cggttctcgt caatatcacg ttttcccaga accagcggac gaataccgtt
    41041 tggatcgcgg aaagcaacca taccgtggcc gataatcatc gccacacagg catacgcgcc
    41101 gcggattaag cggtttgtag cagcgatggc ggcgaaaatg ttgtcggctt caagcgggta
    41161 gtggcggaag ttgtccagct cgctggcgaa gatattaagc agaatttccg agtcggaagt
    41221 ggtgttgatg tggcggcgtt tttcttcaaa cagtttttta cgcaactcgt gagcgttggt
    41281 cagattgccg ttgtgggcaa gcgtaatgcc atacggagag ttaacgtaaa acggctgcgc
    41341 ttcagaggcg ctggagctgc cagccgtagg gtaacgcaca tgaccaatgc ccatattgcc
    41401 ctgcaaacgc tgcatatggc gagcttcaaa tacatcgctc accagcccgt tcgctttacg
    41461 caaacggaag cagttattgg catctatggt gatgatgccg gcggcatcct gaccgcgatg
    41521 ctgaagcacc gttaaggcat cataaatcga ctggttaacc ggcataacac cggcgatacc
    41581 gacaataccg cacatacgtc tttttcctcg ttaagccaca tctcagagca cttacgctct
    41641 gggcaagaaa cttgacgagc tttgcagata atcaaaaaac catctgatga taaaactgaa
    41701 ctgcggaatc agttgtgatt tactccagtc ttcgcttttc gacaccccgg taaaggagtc
    41761 gagaaagaag agaatagcag caacaatcaa cacaccgcgc aatgcaccga aacagacgcc
    41821 cagcacccga tcggtgcctg acaaccccgt tttctccacc agcaggccta tcacgaagtt
    41881 cacgatagcg ccaacgatca gggtggcgat aaacaggacc gcgatggcaa tcccatttcg
    41941 aaccagttcg tcttcaaagc ccgtaaacca gactgacagg taagtgtagt aatgactggc
    42001 aacaaagaaa gcacaacccc atgtcaccag cgataacgct tcacgaacaa agccgcggat
    42061 caggctaacc agagaggaaa aagcaatcac cgcgattatg gcgtaatcaa tccagaccat
    42121 atgtgtccca cgattttacg ccctgtcatc ctgttcgggg cgcattctaa cagaaaaaga
    42181 aaacgtttgc gtagggattt ccttcccgcg catcaataaa aatggcgctg aaaaaatatt
    42241 caacgccatc gactttttat gcctttgcgg catcggacaa tgcgtgtcgg atgcggcgta
    42301 aacgtcttat ccgacctacg gttctacccc tgcgtaggcc tgataagacg caccagcgtc
    42361 gcatcaggca acggctgtcg gatgcggcgt gaacgcctta tccgacctac ggttctaccc
    42421 ctgcgtaggc ctgataagac gcaccagcgt cgcatcaggc aacgcctgtc ggatgcggcg
    42481 tgaatgcctt atccgaccta cggttctacc cctgcgtagg cctgataaga cgcgccagcg
    42541 tcgcatcagg caacggctgt cggatgcggc gtaaacgcct tatccgacct acggttctac
    42601 ccctgcgtag gcctgataag acgcgccagc gtcgcatcag gcaagaccgt attaattcgg
    42661 cgtatagccc attaccacgc cacttaagcc agaaagttgc ttcaactcac ccagcgaacc
    42721 tttcagctta tctttcgagg catccggccc aaccagaata cgggtaattt taccctgcac
    42781 tggcgtggat ggcgacgtat aaacccgata accggcaccg cgcagcttac cgacaatctc
    42841 attcacttta tcggcatttt tcagcgcgcc cagttgcaca acataggctt tacccgttgg
    42901 cgctgctttt tcttccacga ccggcttcgg ttctggcttc ggcgcaggtg gtgcttcaac
    42961 cttcggctta ggcggctcca ccggtttcgg cttcggtggg acgaccggtg caggttccgg
    43021 ttcaaactcg gtgttattgg ctgcaatagt ggccggatcg agcgacggtg ctgcggcatc
    43081 acctgcccgc acctcttccg ctgcgccttc cggcggttgc gtcggcaacg cctgggtggc
    43141 ggctggcatc atatcaggct catcgcgatc gcccgctttt ggcaccagcg ggatagccgc
    43201 aaactcatcc tgataatgtt ttttctgccc gtccagcagc cctggaagta caatcacccc
    43261 cagcgccacc agcacgatcg tgcccactaa ccgattctga aacttacttg ccaccgcttc
    43321 tcctcgcgtc aatcacttcc atgacatgtg cgaccgtgtg gaacgaacca cacaccagca
    43381 cggtgtcttc cgctttagcg tccgccattg cggcatccca tgcctgcgca acgctatcaa
    43441 atgatttgcc gttacccaaa tgctcaagca gttgttctgc cgtggcaccg cgcggccctt
    43501 ccagtggcgc acaataccag tcatcaacca cgcttttcaa ccaggccaga gttccggcaa
    43561 tatctttatc atgtagcata ccgataaccg ccagcacgcg cccgtttttc ggtagcgttt
    43621 tcatacgccc ggtgagatat tccgccgcat gtggattatg cgcgacatca aaaataacgc
    43681 gtggcgactc gctcacaatc tggaaacgtc ccggcaaaat tgcgctggca atcccgtcgc
    43741 gaatggcatt ttcactgacc tccagcccgc tggcacgcag tgccgccagc gccgttgcgg
    43801 cattcggttg cgggacaagc ggcaacggca ggttttccag cgtgccgtga gcatcgctaa
    43861 acgcccagtc atgatcggtg acggaatagt tccactcaac gccccgacgt tgtaacagtg
    43921 cacctttttc ctgcgccaca tcagcaatgg tagaaggcat ttccggctca ccgacaattg
    43981 ccggtttttc gctgcgaaag atgcctgctt tctcgcgacc aatactttcg cgatctggcc
    44041 ccagccagtc ggtatgatcc agcgcaatac tggttactac cgcgacatcg gcgtcgacaa
    44101 tattggttgc gtccagacga ccgcccagcc ctacttccag aatcaccacg tcaagttgtg
    44161 cctgtttgaa cagccacaac gccgacagcg taccgtactc gaaataggtc agggaaatat
    44221 caccgcgtgc cgattcaatc tccgcaaaag aggcggtgtg ggccgattcc ggcaattcct
    44281 gaccctgcac acgtacgcgc tcggtataac acaccagatg aggcgaactg tagacgccca
    44341 ctttgtaccc tgccgccatc agaatcgact ccagcgtacg gcaggtggtg cctttgccat
    44401 tcgtacccgc aacggtaaac acaaatggcg ctggtttcag aacgccaaga cgcgccgcga
    44461 cctggctcac gcgctcaagg ccgagatcga tagttttact gtgcaggttt tccagataag
    44521 aaagccacga agccagaggc gacgcggctt gaggagtgcg tttgataatc atggtatccg
    44581 ctgattcgtt acggtgagaa tagcaaaagg gcagagccag tggccctgcc cttatcagtt
    44641 atcaggcctc aggttcctga tccggtaccg ggggtaccac tacgccttca cgcggcgctt
    44701 caggattcgg cgctggcaga ttcatcaact tcgccagaat gctcgccagt ttcaggcgca
    44761 tttccggacg acggacgatc atgtcgatcg cgcctttctc gatcaggaat tcactgcgct
    44821 ggaatcctgg cggcagtttt tcgcgaacgg tctgttcgat aacacgcgga ccggcaaagc
    44881 cgattaacgc tttcggttca gcgatgttga gatcgcccag catggcgaaa cttgcagaaa
    44941 caccgcccat cgtcgggtcg gtcagcacgg agatgtacgg caagccgcgc tcctgcattt
    45001 ttgccagtgc cgcagaggtt ttcgccatct gcatcagcga catcagtgct tcctgcatac
    45061 gtgcgccacc agaggcggag aagcagatca gcgggcagtt atcttccagc gcctgctcaa
    45121 cggcacgcac gaaacgtgca cccacaacag accccattga accgcccata aaggcgaact
    45181 cgaatgccgc agcgacaacc ggcattccat acagagtgcc tttcatcacc accagcgcat
    45241 ctttttcgcc ggtttctttc tgcgcagatg ccagacggtc tttatacttc ttggagtcac
    45301 gaaacttcag cacatctttc ggctcaagct cgctacccag ctccacaagg cttccttcat
    45361 ctaacaggct atgcaggcga ttacgcgctg tcatacgcat gtgatggtca cacttcggac
    45421 agacctcaag attacgttcc agctcagcgc ggtataaaac ctgaccgcag ctatcacact
    45481 tagtccacac cccttcagga atgctcgcct tgcgggtggg agtaatgttg cttttaattc
    45541 gttcaatcca gctcattagg gacctttctg tctgaacctg gttcgatgcc agttttatct
    45601 ttggggacgc ataatgccat ttttgccccc aacagaccat gaatgttgca cattaaaaca
    45661 taacagcccg aaactttgga taaaaaagtg gtcgaaccgc ggagttactt tttattttgc
    45721 ggcgcgtgcc gcagcgcgtt tgtgacggat tatttcgatg acgcccggca aaatagaaac
    45781 cacaataatc ccgacgatca gcagcttaag gttatcctga accatcggga ttgtaccgaa
    45841 gaaatagcct gcgtaggtaa aaagcagtac ccacaacagt gcgccgatca cgttataagc
    45901 agcgaaatga cggtacgaca tgtggcccat tcccgcaaca aacggggcga acgttctgac
    45961 gatcggcaca aaacgggcga gaataatcgt tttgccgcca tgtttctcat aaaactgatg
    46021 ggttttatcg agataactgc gacggaaaat tttcgaattg ggattactga ataacttttc
    46081 accgaacaag cgcccgatag tgtaattgac cgcgtcaccc acaatcgcgg cgatcagcat
    46141 cagcaccacc atcatatgga cgttgagatc gttggtttcc agtgatgcca acgctccggc
    46201 aacaaacagc aatgaatcac ccggtaaaaa cggcgttacc accagaccgg tttcacagaa
    46261 caaaattaag aacaaaatgg cataaaccca gacgccgtac tccgcgacca gctctgccag
    46321 atgcacgtca atgtgcagga taaaatcgat gaggaaataa atcaggtcca tatttgccta
    46381 tgccttgtac tcgtcatctt tcaggctgta actacgttgg ctacagcctg aaatgttccg
    46441 agcattattc tcgttagtcc gccagaaata gcgggcccat tggcggtttt ggaagatcat
    46501 accggtcagg gtaatccacc gcgaccagat acagcccttc cgcttttgcc gttgctgccg
    46561 ccagcgttct gtcctttgcc gccagcaact ctgctatcca gctctccggc tggttgtggg
    46621 caccgacttc catcaggctg ccgacaatat tcctgaccat atgatgtaca aaggcattcg
    46681 ctttgatatc aaccaccaca taaggaccgt gacgcgtgac attaatatgc ataacgttgc
    46741 gccacggggt tcgggactgg cactgcaccg cacggaacga ggtgaaatca ttctcgccca
    46801 gcaagcactg cgcagcccga tgcatccgtt cagcgtccag cggttcgtaa aaatgggtta
    46861 cccctttact cagtaccgcc gggcgcagcc gatgattgta gatgatgtag cgataacggc
    46921 gagccgtggc gctaaatcgg gcatgaaaat catcaggtac agctttaacc caacgcacgg
    46981 cgatgtcacc aggtaaattc gcatttacgc ccaacgtcca cgcagcgtct ttgcgctgcg
    47041 cggtagtttc gaaatgcaca acctgcccgg taccgtgtac ccctgcgtca gtacgcccgg
    47101 cgcagaagac ggtgatgggt tcgttcgcca cctgggagag cgccttttcc agcttctcct
    47161 gcacactgcg gacttcattc tgccgttgcc agccgtaata cttactgccg tcgtactcaa
    47221 tgcccagcgc aattttataa actggcggtt gttgctggtc ggacattagt acagatactc
    47281 ctgcaccagt ttctcggcga tttttactgc catcagcgcg ccgccaaagc gaacgttatc
    47341 ggccaccgac cagaactgga cttgctccgg cataccgtag tcattacgca cgcagccaac
    47401 agaaagatgc ggcgtacccg aagcatcacc tacctgagtt gggaattcgt tctcttcaga
    47461 gagcacaata tcttcccctt gaacaaacgc atcacgcgct tcttctgctg ccagtggacg
    47521 cagagcttca aagttgacca tctgggcatg accgtagaat accggtgcct ggacgacgct
    47581 tgccgaaatc atcagtcctt cgtcctgcag gattttgcgt acttcgtcaa cgatacgacg
    47641 ttcttcacgc acgctacctt cgctatccgg cagtaacggc agcatgttga acgcgagctg
    47701 acgcccgaag aaatcttctt cgtcaatcgg aatgccgttg agcaatttcg cactctgccc
    47761 cgctaacgca tcgaccgctt ttttgccctg ggcggaggct gaaatcaggc tggtaacact
    47821 gatacgcgac aaaccgccct gatcgattaa cggtttcagt gccgccagca gctggctggt
    47881 cagactgtct ggtacggcga tgacattccg gttgcggtaa tcggtcagta caaacgggtt
    47941 tacttccggc accaccagcg gtacgtcggg ttcaagagca aacaatccgc tgctgtcgat
    48001 caccaggcaa cctgagttgg tcgcttcttc aacccaggct gcggtagctt ctttgcctgc
    48061 gacaaaaaat gccagctgcg cctgcgtcca gtcgaattca gcagcatcct gcacggtgat
    48121 tgtcttacca ccaaagcgca attgttcgcc tgcgctttcg ttacgtgcca gtgcataaat
    48181 ttccccaacc gggaactgac gttcagccag cgtttcaagc agggcttcgc ccacagcgcc
    48241 agttgtgccc aggacggcaa tgttccagcc ttcagacatg gtggtttact ccagaaatag
    48301 caaagctccc tgccaatgtt atagcagaga gcatgaagaa gagattaacg tgccggatga
    48361 tgaacggcgt taaaacccag tttacacagc aatgatgccg cactggcgtc atcacaaatt
    48421 acatacagag atgaccattc acggcgctca agatagtttt tgcgcagttt atcgaactca
    48481 cccggtatcc cggcgacttt acgcagcggt gcgtcatcgc ggcgcacatc atacaccaaa
    48541 tgcaccagcc ttttcagcgt cggttgatcg agcggtccat gcagagtaat gcgaccaaac
    48601 tctggcgcag gcagtaatgt atccagcgca acgtgctgtt catgcccaat aaacttgcta
    48661 taggcttcaa acacttgcgt agtaccgcgt gctttacctt ccagggtata gcctgcaata
    48721 tgcggcgtgc cgatatccac ttttgtcagc aattccacat ttagttccgg ttcgccttcc
    48781 cagacatcca gcactacgct taacttctgg ccttcattca ggcaggtcag cagcgcggta
    48841 ttatcgacga ctgcgccacg gcaggcgtta atcagaatcg ctccgggctt caggctacgg
    48901 atcagttttt catccgccag atgcagcgtt ttgtacggcc cgtctttgaa gagtggcgta
    48961 tggaaagtca gaatatccgc gcgctggact aactcatcca gcgagcggaa atcaccctca
    49021 tccccacagt cggcgcgagg cggatcgcaa agtaaggttt taatccctaa cgcttccagt
    49081 cgtgcctgta aacgccgacc aacgttacca acgcctacga tccccacggt acgctcgtgc
    49141 agtgaaaatc catcgcgttc ggcaagcatc agcagggagg agaaaacata ttccaccacc
    49201 gcaatcgcat tacagccagg tgcagcggaa aaaccaattc ccgcctgctt caaccatgct
    49261 tcatcgacat ggtcggtccc cgcagtggca gtgccaacaa atttaatggg ttttcctgcc
    49321 agcaaagatt cattcacttt cgtgaccgaa cgcaccatca gcgcatccgc gtctgccagt
    49381 tgagcgacgg ggattggacg cccgggaacc gcgatcacct cacccaaacg gctaaataag
    49441 tcgcgggcat aaggcatatt ttcatcaaca aggattttca cgtttgtgtt acctgtatga
    49501 gacgagagtt aaccggacaa gtgtgccata atctcgcggc caggcatact tgcgaagatt
    49561 tcaggtataa ggatacgtaa tgatacaacc tatttccggc cctcctcctg ggcaaccacc
    49621 aggtcaggga gataacctgc cgtctggcgc gggcaatcag cctttatcca gtcagcaacg
    49681 tacttcgctg gaaagcttaa tgacgaaagt gacctcactg acgcaacagc aaagagcaga
    49741 actgtgggcg ggtatcaggc acgatattgg tctgtcggga gattcaccgc tgctttcgcg
    49801 tcactgccct gccgctgagc ataatctggc gcaacgtctg ctggccgcgc aaaaaagcca
    49861 ttctgcccgc cagcttttag cgcaattagg ggagtattta cgtctgggga ataatcgtca
    49921 ggcggtcacg gattatatcc gtcataactt tggtcagacg ccgctgaatc agctctcacc
    49981 ggagcaatta aaaaccattc tcaccctgtt gcaggaaggg aagatggtta ttccgcaacc
    50041 gcagcagcgc gaggcgaccg atcgtcctct attaccggcg gagcacaatg cgctcaaaca
    50101 gctggtgacc aaacttgcgg cggcaacggg ggagcccagc aaacagatct ggcaatcgat
    50161 gctggaactt tccggggtga aagatggcga gttaattcca gcgaaactgt ttaaccatct
    50221 ggtgacctgg ctgcaggcgc gtcagacgct aagccagcaa aatacgccga cgctggaatc
    50281 actacaaatg acgctaaaac aacctttaga tgccagtgaa ctggcggcgt tatcggcata
    50341 tatccagcaa aaatatggcc tttctgcgca atcatcgctt tcttctgccc aggccgagga
    50401 tattcttaat cagctttatc aacggcgggt taaagggatt gatccgcgtg atatgcaacc
    50461 gctgcttaat ccttttccac cgatgatgga cacgttgcaa aatatggcaa cgcgtcccgc
    50521 gctgtggata ctgttagtcg cgattatcct gatgctggtc tggctggttc gttaaccccg
    50581 acgaaacgac agtatcgtga caataattcc cagcaccgca gagatcgccc cggcaagaaa
    50641 taccgaagag taaccaaacg tggtcgccag cattcccgca agcggcccgg agacgccgag
    50701 ggcgatatcc tgaaacgcgg cgtaaccgcc cagtgcggtg ccgcgaactt gtgaggggac
    50761 gcgtttaacc acctccacgc ccagcgcagg aaagataagc gaacatccgg ctccggttaa
    50821 cgccgcgccc gctaatgcga cccacgcccc tggggcttgc cagagcagca acaagcccac
    50881 cgtttctaca agcagagaga caatcgccac tttcacgccg ccaaaacggt ccggcatcca
    50941 gccaaacatg acgcgcatca cgacaaatgc gccgccaaac gcggtaagag taaagcccgc
    51001 catcgcccat cctttgctgg caaagtagag cgaaacgaaa gtcccgataa ccgcaaaacc
    51061 aacgccttgt agtgccagac ctaaccctgg tttccagata agtccgacaa cgctccacag
    51121 agatggacgt tctcccgcca gggctggtac tttgcgcact gtgccgttac aggcccacgc
    51181 cagtaagggt aatgccattg tggtgagcgc cagtgcggca aaaccgtaat ggctatgaat
    51241 caacaggcca agcggagcac caacagcgag ggcaccgtaa atcgccattc cattccatga
    51301 catcactttg ccagagtgtt ttggccctac gatgcctaac ccccaggtca gagcgcctgt
    51361 cagtaactgg ctttcaccaa acccaagaat caaacgcccg atgaccaaca gggcaaattt
    51421 gaacggtgcg gagacaggca aaatcgccgc cagcagcaac gcgccgccag ccagaccaca
    51481 agctaacatc ccctgaagcg ccgaacgttt tgcgccatat tgatcggcca gtcgcccggc
    51541 atagccacgc gtcagcaccg tagccagaaa ctgaatgccg acggcaatgc cgaccatggt
    51601 attgccatag cccagatcat gatgaacaaa caacgggata accggcaacg gcaaccctac
    51661 ggtcatgtag gtgagaaaaa ccgcaaaagc gatgcggaag agcgaaaaat tggcagaaga
    51721 tcgtgtttcg gtttggctta cagcagtcat gcattactcc agaatgcagc gcaaggcgag
    51781 gagtatcccc gtctcatctc tctggtttca gggttacagt gcgttggcag gatttaacgc
    51841 gtacgtcttt tcagaaggaa atcgacaaag cggggagttt gcctggaact ggcggcgatt
    51901 gtcaatgatg tgaaaaaggg aaccatcagg ttcccttttg cgttagtgcc ggaggccgca
    51961 ttggcgcgta acgtcggatg cgacgctggc gcgtcttatc cgacctactc cggcctactg
    52021 cgaattaatc tttcagcttg cgcattacca gcgtggcgtt ggtgccgccg aagccgaagc
    52081 tgttagacat aacagtggtc agttcgcgat cggtcgtttc ggtcacgatg ttcagacccg
    52141 cagcctgctc gtccagctct tcaatgttga tgctcggggc gataaagccg tgttccagca
    52201 tcagcagaga gtagatagct tcctgtacgc cagcagcgcc cagagagtga ccggtcatgg
    52261 ctttggttgc agaaatcgcc gggctcttat cgccgaacac ttcacggata gctgccagct
    52321 ctttcacgtc gccaaccgga gtcgaagtgc cgtgggagtt caggtaatcg attggggtat
    52381 caacgccatg catcgccatc tgcatacaac gtactgcgcc ttcgccagag ggagcaacca
    52441 tgtctgcgcc atcagacgtt gcgccgtagc caacgatttc agcatagatg tgagcaccac
    52501 gcgccagcgc gtgttccagc tcttcaacca ccaccatacc gccgccgcca gcgataacga
    52561 aaccgtcacg gtgagcatcg taagtacggg aggctttttc cggggtgtcg ttgtatttag
    52621 tagacagcgc acccattgcg tcgaattcgc aagccatttc ccagcacagc tcttcgccgc
    52681 cgccagcaaa cacgatgtcc tgtttgccca gttggatctg ctctactgcg ttaccgatac
    52741 agtgtgcgga agtcgcacac gcggagctga tggagtagtt aacgccatga attttaaacg
    52801 gcgtggcgag gcaggcagaa acgccggatg ccatcgcttt ggtgaccaca tacgggccaa
    52861 ccgctttcag gccgcgcggg ccgcgcattg cgtcagcgcc gaacacctgg aaacgcgggg
    52921 aaccgccgcc agaacctgca atcaggccaa cacgcggatt attctggtaa gcttccggag
    52981 agaggcccgc atctgcgatt gcctgctcca tagaaaggaa tgcataaatg gatgcgtcgc
    53041 tcataaagcg cacaactttg cggtcaatga ggccagtggt atccagtttt acgttgcccc
    53101 agacgtggct acgcatgccg gaatccttca gctcctgaga gaaagtgatc cctgaacgtc
    53161 cttcacgcag agatgccagg acttcctgct ggttattacc gatgctggaa acaatgccca
    53221 ggccagtaat cactgcacgt ttcattcaat acctctgtaa gtcgcacata gagtaagttt
    53281 cgaatgcaca atagcgtaca cttgtacgcc gaacaagtcc gatcagccaa ttaatagaga
    53341 aatttgcgca gccttacaca catcgctaag atcgagctac cgcctgtaag acgagtaact
    53401 tacgtgaaac actactccat acaacctgcc aacctcgaat ttaatgctga gggtacacct
    53461 gtttcccgag attttgacga tgtctatttt tccaacgata acgggctgga agagacgcgt
    53521 tatgtttttc tgggaggtaa ccaattagag gcacgctttc ctgaacatcc acatcctctg
    53581 tttgtggtag cagagagcgg cttcggcacc ggattaaact tcctgacgct atggcaggca
    53641 tttgatcagt ttcgcgaagc gcatccgcaa gcgcaattac aacgcttaca tttcattagt
    53701 tttgagaaat ttcccctcac ccgtgcggat ttagccttag cgcatcaaca ctggccggaa
    53761 ctggctccgt gggcagaaca acttcaggcg cagtggccaa tgcccttgcc cggttgccat
    53821 cgtttattac ttgatgaagg ccgcgtgacg ctggatttat ggtttggcga tattaatgaa
    53881 ctgatcagcc aactggacga ttctctaaat caaaaagtag atgcctggtt tctggacggc
    53941 tttgcgccag cgaaaaaccc ggatatgtgg acgcaaaatc tgtttaacgc catggcaagg
    54001 ttggcgcgtc caggcggcac gctggcgaca tttacgtctg ccggttttgt ccgccgcggt
    54061 ttgcaggaag ccggattcac gatgcaaaaa cgtaagggct ttggacgcaa acgggaaatg
    54121 ctttgtgggg tgatggaaca gacattacca ctcccctgct ccacgccgtg gtttaatcgt
    54181 acgggcagca gcaaacggga agtagcgatt atcggcggtg gtattgccag cgcattgttg
    54241 tcgctggcgc tattgcgtcg cggctggcaa gtaacgcttt attgcgcgga tgaagccccc
    54301 gcgctgggtg cttccggcaa tcgccagggg gcgttgtatc cgttattaag caaacacgat
    54361 gaggcgctaa accgcttttt ctctaatggg tttacttttg ctcgtcgact ttatgattca
    54421 ttacccgtta aatttgatca tgactggtgc ggcgtcacgc agttaggctg ggatgagaaa
    54481 agccagcata aaatcgcaca gatgttgtca atggatttac ccgaagaact ggctgtagcc
    54541 gttgaggcaa atgcggttga acaaattacg ggcgttacga caaattgcag cggtattact
    54601 tatccgcaag gtggctggct gtgtccggca gaactgaccc gtaatgtgct ggaactggcg
    54661 caacagcagg gtttgcagat ttattatcaa tatcagttac aggatttatc ccgtaaggat
    54721 gactgttggt tgttgacgtt tgcaggagat cagcaagcga cacacagtgt agtggtactg
    54781 gcgaacgggc atcaaatcag ccgtttcagc caaacatcgt ctctgccagt gtattcggtt
    54841 gccggtcagg tcagccatat tccgacgaca cccgaactgg caaaactgaa acaggtgctg
    54901 tgctatgacg gttatctcac gccgcaaaat ccggcgaatc agcaccattg tattggtgcc
    54961 agttatcatc gcggcagcga agagacggcg tacagtgaag acaatcagca gcagaatcgc
    55021 cagcgattga ttgattgttt cccacacgca caatgggcga agacggttga tgtcagtaaa
    55081 aaagaggcgc gctgcggtgt gcgttgtgcc acccgcgatc atctgccaat ggtaggtaat
    55141 gttcccgatt atgaggcaac actcgtggaa tatgcgtcgc tggcggagca gaaagataag
    55201 gcggtaagcg cgccggtttt tgacgatctc tttatgtttg ctgctttagg ttcgcgcggt
    55261 ttgtgttctg ccccgctgtg tgccgagatt ctggcggcgc agatgagcga cgaaccgatt
    55321 ccgatggatg ccagtacgct ggcggcgtta aatccgaatc ggttatgggt gcggaaattg
    55381 ttgaaaggta aagcggttaa ggccgggtaa tctgctctcg cagtgtttgc cggatgcggc
    55441 gtgaacgcct tatccgacct acgtgtgacg tgtaggcctg ataagacgcg ataagcgtcg
    55501 catcaggcat ggtgctccag acgccgcaac ttactgctgt gacgcctgct gaaacaggtt
    55561 ttcccacata tcggtcacca gcgcctggtc acgcggcgac aattcaccag caccaatggc
    55621 tttttccaga ctctggctaa cggtcgtatg caccgcctga gcggagtggt catcaccact
    55681 ttccagttct gcgatggcta atgtaaggtg gccacgcaaa tacccactgg caaacaactc
    55741 atcatcactg gcatggtcaa ccataccgtc gattaattcc agaatgcgtg attcaaactc
    55801 cgcgatcatc ttctttcctc attgtaaaac gtggcgctgg cttcagttga gcgcttccgg
    55861 ccacgggaac tgttccgccg taagttccgg cgtgtgataa taattttgta gtgctttaat
    55921 gaaacgtgcc gggcgttccg gaatgccatt tgttagataa tccatcacct gcgcatgtac
    55981 tcgccgttga aacaccacgc ggtccggttc gaagtcgcct tccagattgt cgcaactgac
    56041 gttaaacggg tatcccgccg ccacgcagaa caaccagtcg agcgcctgcg gcttcacttc
    56101 aacatcttca aactggcttt gcgtttgggc atcgcgaccg tccgggcaat accagtagcc
    56161 gaaatcaacc tgctcgcggc gcgctttccc ggcaatacac cagtgcgaaa tctcatgaat
    56221 tgcgctggca taaaagccgt gggcaaagac gattcggtta tacggtactt ccgcatcagc
    56281 gggaagatag atcggttcgt cgtcgccttt aatcagacgg gtattaaaat catcggcaaa
    56341 gcagctatta aaaatttcaa tcaactgctc atagtggtgt gtactgttca ttagttcatc
    56401 cccaaccagt ggaggatctc ctgtccgtgg ctatcataaa gtaatttggc actcatcacc
    56461 gccgagacga taacgatcat cgggcggatc agtttttgtc ctttgctcaa caccagtctt
    56521 gaacccatgc gcgcgccaag gaactgcccc accagcatca caaagcccgt cgcccaaatc
    56581 actttgccgc cgagaataaa cagcagtaaa ccgccgatgt ttgacgttgc gttgagtaat
    56641 ttggcgtgag ccgtggcttt ggcgaggttg aatccgcaca gcgtaacaaa ggccagcgcg
    56701 taaaacgatc cggctgccgg accaaagaat ccgtcataaa aaccgacgca gccaccggca
    56761 attaacgcga agggcaaacc gtacatccgg cgctggcgat cttcttcacc cagctttggc
    56821 atcagcaaga aatagaggcc gatacaaatc accagaatgg gcaaaatctg ccgtaagaca
    56881 tcagcctgaa cgtactgtac caacagtgcg ccgctcattg agccgacaaa ggtcatggca
    56941 atattaagtt tctgatcgct taaactaacc actttgcggc gaataaagta gatagtggcg
    57001 gaaatagagc cgccgcaggc ttgcagttta ttggttgcca gagcattagc gggagacatc
    57061 cccgctgcca tcaatgccgg aatggtgagt aacccaccgc caccggcaat cgagtcgata
    57121 aatcccgcca gcatggcgac aaaaaagaga actcccagca acagcgggga aaccataaac
    57181 aggctattaa acgtttccat tagatcacgt gctcatccag tagcgcctgg caggaaggcg
    57241 gcaacggagg cggtgtcttc ttctcaggct ttgttgttcc cggttttgga ggttcaaacc
    57301 agctttgcag ttctgccccg caaccatcgc ctggtggcgg taaaggttga tcttcacact
    57361 ccagactatc ggcaggacaa cgtaatcgta catgcatatg cgcacgatgc tggaaccagg
    57421 gtcgcacttt gcgcaaccag tcgcgatcgg tgcccgcatc aaggcaaagt tgttgtttaa
    57481 tcgccggatt aacaaaaatg cgcgtgacgt ctttgtcctg ggcggcgagt ttgatcaagc
    57541 tgaaaatttc tggcttccac agcgtggaga caacgtgttt accgtcgcgg gaaactaagt
    57601 ccagtgcttg cgggcgcaag agctgcgcgg aggtccggcg tgttttcggc agttgcagaa
    57661 agatatcgac atccagtccg gtctggtggc tggcgtgacc gccgttgaaa cgcccaccag
    57721 caggcattcc catatcgcca atcaacaccg tacccatgcc cagattgctc acctgactac
    57781 tcaggcgctg gataaacatc accaggtccg ggtgaccgaa atagcgacgc tgatcggtac
    57841 gcatgacctg ataatgttcg gactgtatcg gcaacgtgtc agcgccgacg atacaaccat
    57901 tggaaaaact gcctatcgat tgtgcgctac ccggcacagg ttgggttatt ttttgccacg
    57961 gcgtcgctgc caggctgacg ctactggcaa gcagggccag cagcgcaatc gcggttttgt
    58021 tcatttttta ccagcgtgga atatcagtct tcacatcggc attttgcgcc cgctgccgta
    58081 acaggtgatc cattaaaacg atcgccagca tcgcttctgc tatcggcacc gcgcggatcc
    58141 cgacacaggg atcgtgacgg cctttggtga tcatctcaac ttcttcgcca aagcggttaa
    58201 tggtgcgccc cggcacggta atactggagg tcggtttcag tgccatatga gcaatgattt
    58261 gctgcccgct gctaattccg ccgagaatgc cgcccgcatg gttactctgg aaaccgtctt
    58321 tggtgatttc gtcgcggttc tggctgccac gcagcgccac cacgtcaaaa ccatcaccaa
    58381 tttccacgcc tttcaccgcg ttaatgctca ttagcgcatg ggcgatgtcg gcatccaggc
    58441 gatcaaagac cggctcacca agcccggcag gaacgccact ggcaacaacg gtgactttcg
    58501 cgccaataga gtcgccctct tttttcagcg cgcgcatcag ttcatctaac gcgtcgattt
    58561 tgtccgggtc cgggcagaag aatggatttt gctcgacctg cgaccagtct ttgatttcca
    58621 gcggaatgtc gcccatctgg gtcaggcagc cgcgaatttc gatgccaaat ttctcagcga
    58681 gatatttttt ggcaatcgcc cctgctgcaa cgcgcatggc ggtttcacgg gcggaagaac
    58741 gtccaccgcc gcgataatcg cgcagaccgt atttttgttc gtaggtgtaa tcggcatggc
    58801 caggacggaa aacgtcctta atcgcactat aatcctgaga acgctgatcg gtattttcga
    58861 tcaacaaacc aatgctggtg ccggtagtaa caccttcaaa aacaccggag agaattttga
    58921 cctgatctgg ctcacgacgc tgggtggtat agcgcgatgt cccagggcga cgacggtcga
    58981 ggtcatgttg caggtccgct tccgtcagcg gaatgcctgg cggaacacca tcgacgatgc
    59041 agccgagcgc cagcccgtgc gattcgccga aggtggttac gcgaaagagt tgtccaattg
    59101 tgtttccagc catcacggct ccgttattgt tgtgtttgcg tgtttactta atctttataa
    59161 atcgcgaaat gttcacgggc cgccagcagc tgcgctttgg tgagcataaa cacaccatcg
    59221 ccgccgttat caaactccag ccaggtgaac ggaacatcgg gatattgttc cataagatgt
    59281 accatgctgt tgccgacttc acaaatcagt acgccatcat cagcaagata atctgccgcg
    59341 tttccgagaa tgcgacgcgt cagtttcagg ccgtcggtgc cagatgccag gcccagttcc
    59401 ggctcgtggc ggtattcatt tggcaggtcg gacatatctt ccgcatcgac atacggcggg
    59461 ttagtgacaa tcaggtcgta ctgcactttc ggcaaatcac ggaacagatc ggaacgaatc
    59521 ggtatgacgt tgtggatcag accgtgttct tcgatgttct gttcggcaac cgccagcgcg
    59581 tcgggagaga tatctaccgc atcgacttcc gcttccggga aggcataagc acaggcaatg
    59641 gcgatgcagc cgctaccggt acacatatct aaaatatgct gcggttgctt gctgataagt
    59701 ccggcaaatt tattgttgat caattcacca atcggcgagc gcggcaccag cacgcgttca
    59761 tcgacgtaaa attcatggcc gcagaaccac gctttgttgg tcaggtaagc taccggaatg
    59821 cgttcgttga cgcggcggat cacgcgctca acaatacgat gtttttcgct ggaggtcaga
    59881 cgcgcggtgc gcatatcttc cggaatatcc agcggcaggt agagcgacgg caataccagc
    59941 tgtacggctt catcccacgg gttatcggtg ccgtgaccgt accagatatt tgccgcgctg
    60001 aagcggctca ccgaccagcg caacatgtcc tgaatggttt gcagctcatt tactgcttca
    60061 tcaacgaaaa ttttatccac gtattcctcc agggcatgat cgcaataatt tcggcggcta
    60121 gtttgccatg aagatgacga taaatcagca ttcacgcgcg gtgagtgagg aaaaatacgt
    60181 ttaaaacgat cgattgcgct acgagtcggg taaactgtag gaaaattaga aatagagaca
    60241 gataaatgaa aaagaaaaca acacttagcg aggaggacca ggctctgttt cgccagttga
    60301 tggcggggac tcgcaagatt aagcaggaca ccattgtcca tcggccacag cgtaaaaaag
    60361 tcagcgaagt gcccgtcaaa cggttgattc aggagcaggt tgacgccagt cactatttct
    60421 ctgacgaatt tcaaccgtta ttaaatgctg atggcccggt gaagtatgta cgccctggtg
    60481 tagaccattt tgaggcgaaa aagctgcgcc gcggcgatta ttcgccggaa ttgtttttgg
    60541 atttacacgg tctgacgcag ctgcaggcca agcaggaact gggcgcgttg atcgccgcct
    60601 gtcgccgcga acatgtgttt tgcgcctgcg tgatgcatgg tcacgggaaa catatattga
    60661 agcaacaaac accgctgtgg ctggcacaac atccgcatgt catggcgttt caccaggcac
    60721 caaaagagta tggtggtgat gccgcattgc tggtgttgat tgaagtggaa gagtggctac
    60781 cgccggagtt gccatgaata ttagggggcg gatgcgtttt gccatttcgc cctgaatgct
    60841 ggtatcgaat gccggatgcg gcgtaaacgc ctgatccggc ttatcaaaat gtgcaaattc
    60901 aatacgttgt gattcagttg taggcatgat aagacgcgtc agcgtcgcat caggcatcaa
    60961 cgcacaacta cagttcattc agtacatact ggaaacggca tgtagaatca attggctcac
    61021 ggctactggt attcacctgt accacaatgt cgtgtagctt tctatccgtc aatggttgac
    61081 gtgagggggt gactgtcacc tttgcattat cacaaccata cgaggtaaag gttaataagt
    61141 cagaaacagc acgacaatat gcccctctgg aatcaaaaat actgccatcg cagagtttgc
    61201 cgctttccat caggtaggtc gaaagcgtag tcgtgattgt cgaaccatcg gattgcaatc
    61261 gcagcgcttt attatggcta acataattat cactccagga tatatcaaac gaagcgacgg
    61321 gaattgtcgg gcaaatagaa tcatgaactg ttgggcttgt atacgtacca aatccaaatg
    61381 cctcagatac tgaataaaat gaggcaatcc acggacaacc ataattatca gattctacag
    61441 tgacgttaaa agtcatatat gcacagacaa agacacgagt attgatacca gggcaatttg
    61501 aggtgtccgt acgtctataa tcagtcaaaa ccagtgtcgg tgccgggcca gatccgctcc
    61561 aggatattaa cttgagggtg tcgccttttc ttgcattgga gttgtattgt ccgtagctta
    61621 cttctcccgg cgtcactacc gtgacataga aatataaact gccggaagca gtaacgtttt
    61681 tcatcgcata ataaatttta gtactggaaa agcttgcact catgagttta tcaccggcat
    61741 agcactttgg tgtttctaaa ctgagagcaa gtaatatcag acttgtcaaa aatatcgtcc
    61801 attttttcat tttaaaaata ctcaaaagtt accgtcattg tggcggtgaa atcacccgat
    61861 gtaacatttc gtccatttac ggtctgtaac caggcattaa aattgacact gttatttccc
    61921 tgatttaatg gaaatgaggc acctgtggta ctgttaatag gtacagccgc ccctccggca
    61981 gtttcaatgc caatcccaac acccgttgca gaagatgacg aatcaatcga cagaaatcct
    62041 ggtaagtcgg tatcttctgt tcccatcaag gtcaccttga cattaaacgc cgtggtgctt
    62101 ttgcaatctt tcaactgaaa aactaacggt acgcgatctg actgaccacg ttgcattaaa
    62161 tcgctggcag caattgtggg gaaatgaatt tctgcaagta agttgccatt gattacagga
    62221 gtacaggatt taccaagcaa attaccggta aaatgcaggt tatcggcagc tggggccgtt
    62281 ccgctgaaca atatcagacc actgataagc gcccacgcac ggttattctt cattggtaat
    62341 ccacctgtaa cgtagcccat gcttcaaagt ctgactcggg taagtcgaca ccgctctttt
    62401 ttacgggtac tgccgttagt accggaaggg atgtttcatt caccactaag ggcgtatgca
    62461 gcttaaaatc gctcccggct tgtttgaggt gtattcctaa tccattgaga tccgtggcta
    62521 cggccgaatc gtcaaagtca ctctgcgtac cactccaggt caacgtcatt tccatttgag
    62581 ggtcacggac agcggtatcg caggtaatgg tatacggcac gacctgttcg taattgctac
    62641 cgttaatgtc atcaatattc acgtcccgaa acgtcaactc tatattttgc ccattactaa
    62701 tagagcaatt gggtggctgc actaaataac catgaaaaga gacattcgag agcgccataa
    62761 agctaaagct ccacaacatt aagagagaga tccgtttcat tgataatcca ccacaattgt
    62821 ggcactggcg ctaaattccg ccgccgttag cgatacaccg ctctgcacga caggaaccgc
    62881 ttccagagcc agtgcgccag atgaaaagtt aaacggtaac catgcgccag gcgttaaatc
    62941 caaaatggag tgatcgctta ctttctgaat acggatacca aaaccggcaa tacccgtatc
    63001 gattaccgtt tctccgttaa ttacggttgt ggttgcctgc atatttaagc gcaggtcgtc
    63061 cattgccaga ttgttgcaca caagcgagta gtcaatgggg cgtttgtaac ttaccccatc
    63121 gatcttggaa atgaagacat taccaaactc cacgctcgca cccgttaccg tacagggcgg
    63181 cggggcatca acgagcacac gcaagaccat cgcaaccgat ttactccccg ccattacagg
    63241 cgtgctcaca atgctacaca gcaataacca cgcccaggcc gaataagctc ctttcatgct
    63301 attgctccta tccctggcct ttgctgtttt gctcttcgtc cacctgacag gtatttcccg
    63361 tacagcggaa gaacagcggc aaacgcgcac cataatcatt cacataggtc agcaccggga
    63421 cgctatccat tttgactttt aacggcacag aggtttttgg cgatatcacc atgggggaaa
    63481 atcccgctgc cggattgcca tttttctgcg cactggcgtt actgataacc acgaagtacg
    63541 gcgtcgggtt attgacggta aactcctgac cactgagggt taaggtcacc ttatgctgcc
    63601 acgggttttt catgctgacg ttttccagcg cctttggtcg ccagaacaac ttaatgcgcg
    63661 tctgcagcgc tatctgtagc gtgttaggtt tgttcgattt cggcgggatc tcacgcacgt
    63721 tgaaatagaa cacgctctcg cggtcggcag gcagcttgtt gatatccggc atgccctgta
    63781 ccttcacctg accgttcatc atggaatcga tgcgctgcac tggcggcagg accgtcagtg
    63841 gcgagctaat cctgttaccc tgtccatctt ccatccagct ttgtgccaga tacggcattt
    63901 tcgggtcgtt attgcgcagc gtgacgctga ttgatttatc actttcgtta aaaatcaggc
    63961 gtgtacggtc cggcgtgaca gaggcctgta ctgacaggct ggcggcagtt atcagtaacg
    64021 ccagcgtcgt tgcggccgtt ctggcagagc aaaatagatt cgtcatggat cattccttaa
    64081 tcacgttatt aattggctga taccgatact ggcgcttccg ttggcatcac ctgctgggtc
    64141 tgctcctgga tcaccggctt aatttcatga ggcatcgaag gagatatcgc ccctgtttgc
    64201 tggcatggta ataacaggcc attgaacaga tcggctggca gcgggtcagg cagatgaata
    64261 tcgcagtggg ccacaccgcc ccatgaaacg atcatatgct cgccaggttt tacgcccgcg
    64321 aggtagacgt tgccgtcatc gtcaaccaga ccgacgttct gcgcgctgtc gtttttcact
    64381 tccgcgccaa acggcggata actgccatct tgcagacgca gcaccgccat cgctttttgc
    64441 ccgctgatca ccgagaactt acggtagcca atggcccctt cggtaagcgt ggcctgcacc
    64501 acggactggg tggcttcggc gttttccggc aggttgttta ggtcgatata cgcctggttg
    64561 cggtagtagt tgttgacgtc tgccaccact gccttaccga acatattggt gtaaaccgcc
    64621 gcgccatttc cttccaccgg aacaccagcc acaccgtcgg catcaatcag cagacgcgta
    64681 ccgcccatat tctgggtacg gtggagcgcg ccaccttgtg cggtgagcgt cgcgccgccc
    64741 tgtaaggaaa tacccgccga ggtgtactgc ccttcatggt agttagcgct gagatcgacc
    64801 tgcgccagcg atccgtcgtg gctgtaataa ccgtcaacgc tggagtgatt gtcgctggtg
    64861 cctacgttca actggtaatg ggttgcgtca tcgacacggc tgaaataccc cacctggctg
    64921 ctgtccgaac cgctgccgta gttgccgtta tagctgatgg tgctgctgtc accccacggc
    64981 atactgagcg atatgtacac acctttatcg gcctggttgt catactcgta gcggtatccg
    65041 gtcatggaaa tgctcatgtt gcggatactg cccaggttga agtagtgcga gagcatgacg
    65101 ttgtagttgg tctgttcgtc gcgatcccag taggtatggc gggtgtagtt gagataaaca
    65161 gacacaccgg catccctgaa gttctggtta taggtggcgg tgtacatctc tttgtcgttg
    65221 ccggtgcgca ccatttcgct gtcgctggca tcgagatact cgctcatggt catgaagttc
    65281 tcttccgaga agcggtatcc ggcaaaagtg acgcggctgt tcagttcatc gaagtctttg
    65341 gaatagctca ggcgaaacga gttgccgtcc agtgaacctt tcccgtaggc ggtctcttta
    65401 tccagacggg tatgcgagtg ggtgatatca aaggccaccg caccaaacac agacaggtcg
    65461 cgaccgacgc caagcgccgc cgactgatag tgttcatctg ccagcgcccc gccgtatagc
    65521 gaccagccgt tggcaatccc ccaggaagct tcgccgccgg agaagaaacc gccttccacg
    65581 tggtgccccc actcctgcgg gcggcccatc atcagtttat agcgcacctg gccggggcga
    65641 gtcaggaacg gcatcgaggc ggtgttgatg tcatattcct gcacctgacc gttctgttct
    65701 tcaatgcgga tatgcagcgt accggagacc gaatcgccaa gatcctggat gcggaacggc
    65761 cccgccggga cctgggtttc gtagatgacg cggcccaact ggctgacggt cactttcgcg
    65821 gtggtgtgcg ccacgccgga gatatccggc gcatagccgc gcagattcgg cggcaacatt
    65881 tgatcgtcgg tgctgatact gccacccacg tagttaaagc cgtcgaaaat atcagaattc
    65941 aggtagtctt cgccaaggcc aagtttggct tttagcgacg gtaaggctcg ccaggcgtag
    66001 tagcggctcc actcccagtt tttttgcgtg tcatcaccgt tgataacatc gtcatcattg
    66061 cttttactat gcaaataatc agtctgccag tcggcacgaa gacgccatgc gccgaggttc
    66121 accccaaccg tcccgttacc gctaatttca ttgctgtcat ccccgccatt ttcttcatgt
    66181 cgtgtctggg cggtaatact gtagtcagca attaaaccag atataccgtc atcccagcgt
    66241 gaaggcggat cccagttgat gtcggtatat tcaaggtaag cctggggtaa tgaaatgact
    66301 aacgctgact gactcaggtc agctttaata tcaatgcctt ccagttgacc gggtttcagg
    66361 catttgccgt cgtggatcca ttgcaggttt tttgccacat cctcttttaa gccaaactgc
    66421 gcgaccagtt caggcgtcag gcaggcatag gttttactgg catcgttctc agaggcgtac
    66481 cagtaaatat cgtattcttc cgtcagcggc tgtttattta gttgaacctg taaattgtat
    66541 ttcccaggtt cgacataacc ctggctggaa aatcgcttca gatcgatttt tgtgtcgcct
    66601 tttaactcca gaaaacggga atcaaactga atttcatttt cagcccaggc attgacataa
    66661 ctcccggaca ttgccaatgc aacgcagcag ggaagtaccc gcagtcggaa aagagaatgg
    66721 tcaggcatac acgtatccat ccgtaattat tataatttgc gcgaattcca tttaaaaacc
    66781 ccatagcccg aaagctatgg gggtatcact tagagataag taatctggaa ggtcgtgttg
    66841 gcttcaaaat tacctaaatc tggcgcatca gcagcgccca ccagccaggc tttaaagtcc
    66901 agcgtctgct tcgctttgcc tttgttggtc gccgtatcgt ttacgatttt ctgttcgata
    66961 cccgcgccgc ttttataaga ggttccctga gcgtcaccaa tggccaggct gacattgtta
    67021 aatgccgcac cagtatcggt attgtaaatg gtgtagtaat tgccgctgtt ggtagaagac
    67081 gcgttaccgg tgaaggtagt ggtcatcgtt gtctgggtgt caaacacaca gtcttgcaga
    67141 tggatctgga aagctttcgg tgtagaagtc ccgttatttt tcaggatccc tgaaccaatg
    67201 tcacccatat caacttccag tttgtgatca tcaggaacga tagagcacgg ggaatcttca
    67261 atggtgccgt agaaacgtgc tgtaccattg ttgccagcgg cgattgttga agtagaagcg
    67321 aacgcaccca ttaccattgt tgcagcaata gctgttttta caaacttact cattatttgt
    67381 ccttaaacca taaataat
//
LOCUS       DAZFAB010000014        65089 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZFAB010000014 DAZFAB010000000
VERSION     DAZFAB010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65089
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.014"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..295
                     /locus_tag="ACHJWA_001058"
     CDS             <1..295
                     /locus_tag="ACHJWA_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO4307704.1"
                     /translation="HLIRSNTMAPTPVYADYLNNTTDFRPDPEDIFKCGQTLECGDPR
                     KNIRQKYGHHGLGVNQNQQFDNGVMGLNSRMAVFDENKKIIGSSSGLSCFQPH"
     gene            557..2179
                     /gene="yhiJ"
                     /locus_tag="ACHJWA_001059"
     CDS             557..2179
                     /gene="yhiJ"
                     /locus_tag="ACHJWA_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO4307705.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVLEKVDDWQIERFQQSIQDDKISFTIQTDYSEKYSMLSGMRAHILRR
                     NNHYQFIVTINSKNYGCPLDNTDINWCGIVYLLNNMTVNDNANDVAVTESYKPIWNWK
                     ISQYNVSDIKFETIIKPQFADRIYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQVDTNVNTIISILNRRDIILESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINDNVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDSKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAAIINKKHHTIINSKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRECIGETGTVLGLNARDSKHTGRMFSMHNCQINPSA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            2369..2482
                     /gene="yhiY"
                     /locus_tag="ACHJWA_001060"
     CDS             2369..2482
                     /gene="yhiY"
                     /locus_tag="ACHJWA_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFO4307706.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            2545..3612
                     /gene="yhiI"
                     /locus_tag="ACHJWA_001061"
     CDS             2545..3612
                     /gene="yhiI"
                     /locus_tag="ACHJWA_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO4307707.1"
                     /translation="MDKSKRHLAWWGVGLLAVAAIVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            3609..6344
                     /gene="rbbA"
                     /locus_tag="ACHJWA_001062"
     CDS             3609..6344
                     /gene="rbbA"
                     /locus_tag="ACHJWA_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFO4307708.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVIVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVVRDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVIHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPLLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPSSQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            6344..7468
                     /gene="yhhJ"
                     /locus_tag="ACHJWA_001063"
     CDS             6344..7468
                     /gene="yhhJ"
                     /locus_tag="ACHJWA_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4307709.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLIMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            7541..7816
                     /locus_tag="ACHJWA_001064"
     CDS             7541..7816
                     /locus_tag="ACHJWA_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFO4307710.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            7813..8172
                     /locus_tag="ACHJWA_001065"
     CDS             7813..8172
                     /locus_tag="ACHJWA_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFO4307711.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(8222..9082)
                     /locus_tag="ACHJWA_001066"
     CDS             complement(8222..9082)
                     /locus_tag="ACHJWA_001066"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase"
                     /protein_id="HFO4307712.1"
                     /translation="MPLVNGRILLDRIQEKRVLAGAFNTTNLETTISILNAIERSGLP
                     NFIQIAPTNAQLSGYDYIYEIVKRHADKMDVPVSLHLDHGKTQEDVKQAVRAGFTSVM
                     IDGAAFSFEENIAFTQEAVDFCKSYGVPVEAELGAILGKEDDHVSEADCKTEPEKVKT
                     FVERTGCDMLAVSIGNVHGLDDIPRIDIPLLKRIAEVCPVPLVIHGGSGIAPEILRSF
                     VNYRVAKVNIASDLRKAFITAVGKAYVNNHNEANLARVMASAKNAVEEDVYSKILMMN
                     EGHRLVRKAS"
     gene            complement(9114..9383)
                     /locus_tag="ACHJWA_001067"
     CDS             complement(9114..9383)
                     /locus_tag="ACHJWA_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFO4307713.1"
                     /translation="MLNKTVEVRNSTGLHARPAACLAKAAKKYSCKVTLHYEGNDINA
                     TSMMNIMRAGIKGGKTVEIRCDGDDENEAIQTLTDLFRDRFGEAE"
     gene            complement(9373..10881)
                     /locus_tag="ACHJWA_001068"
     CDS             complement(9373..10881)
                     /locus_tag="ACHJWA_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFO4307714.1"
                     /translation="MPDNSAAIVIDIGTTNCKVTCFSCLDATTLGAHKFVTAKQISPQ
                     GDVDFDIDALWQEVRQAIAQLNAASPLPVRRISIASFGESGVFLDEHGEILTPMLAWY
                     DRRGEEYLATLSEADSAALYDICGLPLHSNYSAFKMRWLLEHYPLRNRRGLRWLHAPE
                     VLLWRLTGEQHTDITLASRTLCLDVRKGEWSAKAAALLHVPCTAFAPLVQPGEHAGWV
                     SESLCKTLGFSQPVSVTLAGHDHMVGARALQMMPGDILNSTGTTEGILQLDTQPTLDE
                     QAKRDKLANGCYSLANQFTLFASLPVGGFALEWLRNTFRLTDEEIAASLTRGHADYLA
                     GNWLLDDIPVFIPHLRGSGSPYKNRHTRGLFYGLGDTLSIDMLIASVSLGLTMEFANC
                     FACFNVPGTSALKVIGPATHNPLWLQLKADILQRPVEAIAFNEAVSVGALLTAAPDIP
                     PPPVAIAQRLLPNRARYHQLQRYQHKWKSWYQLKLQQEGVMPLHHREEHYVE"
     gene            complement(10874..12232)
                     /locus_tag="ACHJWA_001069"
     CDS             complement(10874..12232)
                     /locus_tag="ACHJWA_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFO4307715.1"
                     /translation="MNDIAHTLYNIVQYILGFGPTVMLPLVLFILALCFKVKPAKALR
                     SSLTVGIGFVGIYAIFDILTSNVGPAAQAMVERTGINLPVVDLGWPPLSAITWGSPIA
                     PFVIPLTILINVAMLALNKTRTVDVDMWNYWHFALAGTLVYYSTGSLFFGLLAAAIAA
                     VVVLKLADWSAPLVQKYFGLEGISLPTLSSVVFFPVGLLVDKIIDHIPGLNRIHIDPE
                     TVQKKFGIFGEPMMVGTILGILLGVIAGYDFKKVLLLGISIGGVMFILPRMVRILMEG
                     LLPLSEAIKKYLNAKYPDRDDLYIGLDIAVAVGNPAIISTALLLTPISVFIAFVLPGN
                     EVLPLGDLANLAVMASMIALASRGNIFRTVLAAIPVIIADLWIATKIAPFITGMAKDV
                     NFKFAEGSSGQVSSFLDGGNPFRFWLLEIFNGNLIAIGLVPVIALVLYGIFRMTRSTV
                     YA"
     gene            complement(12309..12590)
                     /locus_tag="ACHJWA_001070"
     CDS             complement(12309..12590)
                     /locus_tag="ACHJWA_001070"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO4307716.1"
                     /translation="MSQTILFVCATGIATSTAVTEKVMEYCKEQGLNVNYSQTNVASL
                     PGNTDGVALVVSTTKVPYELDVPVVNGLPIITGIGEEKVLAQIVSILKK"
     gene            complement(12587..13060)
                     /locus_tag="ACHJWA_001071"
     CDS             complement(12587..13060)
                     /locus_tag="ACHJWA_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO4307717.1"
                     /translation="MQGIHFQENYIQRLPAGLSVEQIIRQLAQPLVAAELVVPDFADH
                     VLKREATYPTGLPTEPPCVAIPHTDHKHVRHNAIAVGILPEPVEFADMGGDPDPVPVR
                     VIFLLALSESNKQLNALGWIMEMIQDTPFMQALLAMETTEIHTVILNKMKERGEI"
     gene            complement(13085..13831)
                     /locus_tag="ACHJWA_001072"
     CDS             complement(13085..13831)
                     /locus_tag="ACHJWA_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO4307718.1"
                     /translation="MKSLSKSSQIPLYQQVVEWIRESIYTGDLVEDDRIPSEYQIMDM
                     LDVSRGTVKKAVAQLVKEGVLIQVQGKGTFVKKENVAYPLGEGLLSFAESLESQKIHF
                     TTEVITSRIESANRYVAEKLRITPGQDILYLERLRSIGDEKAMLIENRINIELCPGIA
                     EIDFNQHNLFPTIESLSQRKIRYSESRYAARLIGNERGHFLDISEDAPVLHLEQLVFF
                     SRGLPIEFGNVWLKGNKYYLGTVLQRREVS"
     gene            complement(14030..14431)
                     /gene="nikR"
                     /locus_tag="ACHJWA_001073"
     CDS             complement(14030..14431)
                     /gene="nikR"
                     /locus_tag="ACHJWA_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFO4307719.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(14437..15243)
                     /gene="nikE"
                     /locus_tag="ACHJWA_001074"
     CDS             complement(14437..15243)
                     /gene="nikE"
                     /locus_tag="ACHJWA_001074"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFO4307720.1"
                     /translation="MTLLNVSDLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLSRLLVGLESPSQGNISWRGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLHAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQAVGEKLTFSSDAGRVLQNAILPAFPVRRRATEKV"
     gene            complement(15240..16004)
                     /gene="nikD"
                     /locus_tag="ACHJWA_001075"
     CDS             complement(15240..16004)
                     /gene="nikD"
                     /locus_tag="ACHJWA_001075"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFO4307721.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLKRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            complement(16004..16837)
                     /gene="nikC"
                     /locus_tag="ACHJWA_001076"
     CDS             complement(16004..16837)
                     /gene="nikC"
                     /locus_tag="ACHJWA_001076"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFO4307722.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(16834..17778)
                     /gene="nikB"
                     /locus_tag="ACHJWA_001077"
     CDS             complement(16834..17778)
                     /gene="nikB"
                     /locus_tag="ACHJWA_001077"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFO4307723.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFATQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFTWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(17778..19352)
                     /gene="nikA"
                     /locus_tag="ACHJWA_001078"
     CDS             complement(17778..19352)
                     /gene="nikA"
                     /locus_tag="ACHJWA_001078"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO4307724.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWVLQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLHIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDEAQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(19463..20050)
                     /gene="acpT"
                     /locus_tag="ACHJWA_001079"
     CDS             complement(19463..20050)
                     /gene="acpT"
                     /locus_tag="ACHJWA_001079"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFO4307725.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(20052..21281)
                     /locus_tag="ACHJWA_001080"
     CDS             complement(20052..21281)
                     /locus_tag="ACHJWA_001080"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HFO4307726.1"
                     /translation="MTRRVVITGMGGVTAFGENWQDVSARLLAYENAVRKMPEWQVYD
                     GLHTLLGAPVDDFTLPEHYTRKRIRAMGRVSQMSTRASELALEQAGLIGDPILTSGET
                     GIAYGSSTGSTGPVSEFATMLTEKHTNNITGTTYVQMMPHTTAVNTGLFFGLRGRVIP
                     TSSACTSGSQAIGYAWEAIRHGYQTVMVAGGAEELCPSEAAVFDTLFATSQHNDAPKT
                     TPSPFDENRDGLVIGEGAGTLILEELEHAKARGATIYGEIVGFATNCDAAHITQPQRE
                     TMQYCMEQSLKIAGLSAQDIGYISAHGTATDRGDMAESLATATIYGDNVPLSSLKSYF
                     GHTLGACGALEAWMSLQMMREGWFAPTLNLNKPDPNCGALDYIMHEARKVDCEFLQSN
                     NFAFGGINTSIIIKRWP"
     gene            complement(21278..22009)
                     /locus_tag="ACHJWA_001081"
     CDS             complement(21278..22009)
                     /locus_tag="ACHJWA_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase FabG2"
                     /protein_id="HFO4307727.1"
                     /translation="MSRSVLVTGASKGIGRAIACQLAADGFNIGVHYHRDATGAQETL
                     NAIVANGGNGRLLSFDVANREQCREVLEHEIAQHGAWYGVVSNAGIARDAAFPALSDD
                     DWDAVIHTNLDSFYNVIQPCIMPMIGARQGGRIITLSSVSGVMGNRGQVNYSAAKAGI
                     IGATKALAIELAKRKITVNCIAPGLIDTGMIEMEESALKEAMSMIPMKRMGQAEEVAG
                     LASYLMSDIAGYVTRQVISINGGML"
     gene            complement(22009..22473)
                     /locus_tag="ACHJWA_001082"
     CDS             complement(22009..22473)
                     /locus_tag="ACHJWA_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-fatty acyl-ACP dehydratase"
                     /protein_id="HFO4307728.1"
                     /translation="MSHYLSPGAYLPHDAPMLLLEDVVSVSDDSAVCRVTVSPSGVLA
                     PFLDPDGNLPGWFTLELMAQTVGVWSGWHRHQQGKNSIELGMILGARELLCTTGILPA
                     GQTLTITVKLLMQDDRFGSFECSINDGEATGRINTFQPTAEELTTLFQQGAS"
     gene            complement(22470..23639)
                     /locus_tag="ACHJWA_001083"
     CDS             complement(22470..23639)
                     /locus_tag="ACHJWA_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HFO4307729.1"
                     /translation="MIYISAVGMINALGNNLDEIATNLTRGVAPGMRPRAGWLQGHPQ
                     AVLAGVDGELPLIPEKFAAHRSRNNQILLAALAQIQPQVDDAIAKYGRERIAIVLGTS
                     TSGLHEGDTHVNLRTHGQPSTTWHYAQQELGDPSRFLSHWLALDGPAYTLSTACSSSA
                     RAIISGRRLIEAGLVDAAIVGGADTLSRMPINGFHSLESLSPTLCQPFGRDRAGITIG
                     EGAGLMLLTREPQPIALLGVGESSDAYHISAPHPQGEGAIRAINQALTDAQLTPDDVG
                     YINLHGTATQLNDQIESMVVNALFGERVPCSSTKHLTGHTLGTAGITEAAISMLILQR
                     DLPLPAQDFSLSPRDPTLPPCGIIEKPQPLARPVILSNSFAFGGNNASILLGRVS"
     gene            complement(23641..24225)
                     /locus_tag="ACHJWA_001084"
     CDS             complement(23641..24225)
                     /locus_tag="ACHJWA_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3261 domain-containing protein"
                     /protein_id="HFO4307730.1"
                     /translation="MIKSTFWRAFALTATLILTGCSHSQPEQEGRPQAWLQPGTRITL
                     PAPGISPAVNSQQLLTGSFNGKTQSLLVMLNADDQKITLAGLSSVGIRLFLVTYDAQG
                     LRAEQSIVVPQLPPASQVLADVMLSHWPISAWQPQLPAGWTLRDNGDKRELRNASGKL
                     VTEITYLNRKGKRVPISIEQHVFKYHITIQYLGD"
     gene            complement(24222..26540)
                     /locus_tag="ACHJWA_001085"
     CDS             complement(24222..26540)
                     /locus_tag="ACHJWA_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HFO4307731.1"
                     /translation="MTNANVLPPSKRPALLWGLVCLVMAVALLILLPQSRLNSSVLAM
                     LPKQTMGDIPPALNDGFMQRLDRQLVWLVSPGKEANPLVAQEWLTLLQKSAALGDVKG
                     PMDAASQQAWGAFFWQHRNGLIDPNTRARLQNGGEAQAQWILSQLYSAFSGVSGKELQ
                     NDPLMLMRGSQLAMAKNGQRLRLMDGWLVTQDPQGNYWYLLHGELAGSSFDMQQTHQL
                     ITTLNTLEKDLKTRYPQAQLLSRGTVFYSDYASQQAKQDISTLGVATLLGVILLIVAV
                     FRSLRPLLLCVISIGIGALAGTVATLLIFGELHLMTLVMSMSVIGISADYTLYYLTER
                     MVHGNDVSPWQSLAKVRNALLLALLTTVAAYLIMMLAPFPGIRQMAIFAAVGLSASCL
                     TVLFWHPWLCRGLPVRPVPAMALMLRWLAAWRRNKKLSLGLPVALALFSLAGMSMLRV
                     DDDISQLQALPQHILAQEKAITALTGQSVDQKWFVVYGDSPQQTLRRLEKYTASLEYA
                     KKEGLISNYRTIPLNSLARQEEDLQLLKTAAPTVTKALQNAGLTAVNPDLNAMPVNVD
                     EWLASPASEGWRLLWLTLENGESGVLVPVEGVKSSALMQEIATYYPCGIAWVDRKSTF
                     DELFALYRYVLTGLLLVALAVIACGAVARLGWRKGLISLVPSVLSLGCGLAVLAMSGQ
                     AVNLFSLLALVLVLGIGINYTLFFSNPRGTPLTSLLAIALAMLTTLLTLGMLVFSATQ
                     AISSFGIVLVSGIFTAFLLSPLAMPDKKRTKK"
     gene            complement(26509..27114)
                     /locus_tag="ACHJWA_001086"
     CDS             complement(26509..27114)
                     /locus_tag="ACHJWA_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HFO4307732.1"
                     /translation="MKFLPLLALLISPFVSALTLDDLQQRFTEQPVIRAHFDQTRTIK
                     DLPQPLRSQGQMLIARDQGLLWDQTSPFPMQLLLDDKRMVQVINGQPPQIITAENNPQ
                     MFQFNHLLRALFQADRKVLEQNFRVEFADKGEGRWTLRLTPTTTPLDKIFNTIDLAGK
                     TYLESIQLNDKQGDRTDIALTQHQLTPAQLTDDERQRFAAQ"
     gene            complement(27111..27533)
                     /locus_tag="ACHJWA_001087"
     CDS             complement(27111..27533)
                     /locus_tag="ACHJWA_001087"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFO4307733.1"
                     /translation="MLNDPRFTAEVELTIPFHDVDMMGVAWHGNYFRYFEVAREALLN
                     QFNYGYRQMKESGYLWPVVDARVKYRHALTFEQRIRVRAHIEEFENRLRIGYQIFDAE
                     TGKRATTGYTIQVAVDEQSRELCFVSPDILFERMGVKP"
     gene            complement(27537..29213)
                     /locus_tag="ACHJWA_001088"
     CDS             complement(27537..29213)
                     /locus_tag="ACHJWA_001088"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO4307734.1"
                     /translation="MSVNFSPCVLIPCYNHGAMMPGVLARLKPFNLPCIVVDDGSDAT
                     TQQQLDSLLAEQPGVTLIRLAENAGKGAAVMRGLQAAADAGFSHAVQVDADGQHAIED
                     IPKLLALAEQQPAALISGQPIYDDSIPRSRLYGRWVTHVWVWIETLSLQLKDSMCGFR
                     VYPVAPTLQLAKHATIGKRMDFDTEVMVRLYWQGNTSYFVPTRVTYPLDGLSHFDALK
                     DNVRISLMHTRLFFGMLPRIPSLLMRRSSCHWARQSEVKGLWGMRLMLLVWRLLGRTA
                     FSALLYPVVGVYWLTASRARKASQDWLARVRQHQPQAAKLNSYQHFLRFGNAMLDKIA
                     SWRGELQPGRDVLFAPGAEAALDVRDPRGKLLLASHLGDVEVCRALAKIQGYKTINAL
                     VFSENAQRFKQIMQEMAPQAGINLMPVTDIGPETAILLKEKLDNGEWVAIVGDRIAVN
                     PQRGGDWRVCWSSFMGQPAPFPQGPFILASILRCPVNLIFALRQHGKLHIHCESFADP
                     LQLPRGERQQALQNAIDHYAARLEHYALQSPLDWFNFFDFWQLPEIQDKE"
     gene            complement(29204..29557)
                     /locus_tag="ACHJWA_001089"
     CDS             complement(29204..29557)
                     /locus_tag="ACHJWA_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymyristoyl-ACP dehydratase"
                     /protein_id="HFO4307735.1"
                     /translation="MKPHEIERHQAQTNHLEIVLHLRADLFWFRGHFAVQPLLPGVAQ
                     IDWAMSYALNLLAPGWRFHSIQNIKFQSPLLPENRVTLALNWQEERQILSFSYQRHDG
                     DARHTASSGKIRLCR"
     gene            complement(29544..30905)
                     /locus_tag="ACHJWA_001090"
     CDS             complement(29544..30905)
                     /locus_tag="ACHJWA_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase"
                     /protein_id="HFO4307736.1"
                     /translation="MKQTLPLARWLTAPRPDDTPIAWLNESTWTLGDLRHDVAQLICR
                     LQQQPGERWALCFENSYLFIVALLATLHAGKTPVLPGHNRVIQLNEQRELFDGVLSDS
                     ELNWQGSLLLVASSPQIATQSFTFAAIAPEAFIELFTSGSTGQPKRAIKPVHLLDREA
                     ELLAERLGARLAGCRVVGSVLPQHLYGLTFRVFLPMALGLPLHAAMLWYVEQFAALSH
                     QHRYIFISSPAFLKRLDTQLSPPPVQVLLSAGGELPWQDVQHTASWLRVWPDEIYGST
                     ETGVIAWRYREQEQRRWLPLPGMQFQAEGDAFRLFSPLIAEDNGMLLDDILQFSEDGQ
                     FHLMGRRGRIVKIEEKRISLQEVEQRLLALDGIREAAAVPVTRGGRQSIGVLLVLDDE
                     AHLQWQNGGGHSQEMTWRRLLRPTLEPVAIPRYWRVIDEMPVNSMNKRVYAQLQELFH
                     EAP"
     gene            complement(30902..31483)
                     /locus_tag="ACHJWA_001091"
     CDS             complement(30902..31483)
                     /locus_tag="ACHJWA_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4307737.1"
                     /translation="MSGIRSLPMIKLLTGLLLLAWPFVIWFGLAHNGLHWLLPLMALL
                     LLLRLRQTRRQAGPLQAVTQLVAVVGIALCVASFMLKTHQLLLFYPVVVNAVMLAVFG
                     GSLWSAMPIVERLARLQEPDLPEKGVRYTRHVTQIWCGFFIINGGIALFTALYADMSL
                     WTAWNGMIAYLLMGTLMAGEWLLRRQVMKRDRA"
     gene            complement(31488..31739)
                     /locus_tag="ACHJWA_001092"
     CDS             complement(31488..31739)
                     /locus_tag="ACHJWA_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO4307738.1"
                     /translation="MTEQQTVYQEVSALLVKLFEIDPQDIKPETRLYEDLELDSIDAV
                     DMIVHLQKKTGKKIKPEEFKAVRTVQDVVEAVERLLQEA"
     gene            complement(31751..32008)
                     /locus_tag="ACHJWA_001093"
     CDS             complement(31751..32008)
                     /locus_tag="ACHJWA_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HFO4307739.1"
                     /translation="MQALYLEIKNLIISTLNLDELTPDDIDTDAPLFGDGLGLDSIDA
                     LELGLAVKNEYGIVLSAESEEMRQHFFSVATLASFIAAQRA"
     gene            complement(31983..32804)
                     /locus_tag="ACHJWA_001094"
     CDS             complement(31983..32804)
                     /locus_tag="ACHJWA_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFO4307740.1"
                     /translation="MSKLMSRLEWTWRLVMTGLCFALFGLGGLLLSVVWFNILLVLVW
                     DTSRRRRLARRSIAASFRLFLTVAKGLGVLDYRIDGAEILHQERGCLVVANHPTLIDY
                     VLLASVMPETDCLVKSALLKNPFLGGVVRATDYLINSEAETLLPRCQQRLAQGDTILI
                     FPEGTRTKPGEKMTLQRGAANIAVRCASDLRIVTIGCSEHLLDKQSKWYDVPPDRPFF
                     TVAVHGRVEINKFYDATSQEPALAARQLNRHLLLQLQQGCLPLSGINNASALSGN"
     gene            complement(32801..33523)
                     /locus_tag="ACHJWA_001095"
     CDS             complement(32801..33523)
                     /locus_tag="ACHJWA_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HFO4307741.1"
                     /translation="MKFALNILDWQARAPGLSETLQWQEWSRQPQAIDPTAPLAKLSE
                     LPMMTARRLSSGSKLAVECGLAMLRRHQPDAVLYTSRHGELERNYRIVHALATEQALS
                     PTDFALSVHNSSVGNLTIAAKQPSVSSSLSAGRDSFQQGLCEVLSLLQAGYQRVLMVD
                     FDGFLPEFYHPQLPSEMPTWPYAVALVIEAGDDWQCETQSAIAGNETTLPQSMLFLQH
                     YLQNVDTFSLPGERVQWRWSHR"
     gene            complement(33564..34622)
                     /locus_tag="ACHJWA_001096"
     CDS             complement(33564..34622)
                     /locus_tag="ACHJWA_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFO4307742.1"
                     /translation="MYEQDSLSALDAITEAQRIAFAPMLFQTALCLRNAGVLSYLDRQ
                     GKHGATLADITEHSHINEYATSVLLDMGLSGRIITCKEGIYYLAKIGHYLLHDTMTRV
                     NMDFTQDVCYQGLFFLADSLNEGKPSGLKVFGDWSTIYPALSQLPDAARESWFAFDHY
                     YSDGAFNAALPYVFANNPTTLYDVGGNTGKWALRCCKYNENIAVTLLDLPQQIVLAKE
                     NIANAGFSDRIDFHAVDMLSDAPLPGEADIWWMSQFLDCFSPEQIISILSKIASVMKP
                     GAKVCIMELFWDAQRFEAASFSLNASSLYFTCMANGNSRFYSAEKFYDYLNKAGFQVA
                     ERHDNLGVGHTLLICQKK"
     gene            complement(34691..35068)
                     /locus_tag="ACHJWA_001097"
     CDS             complement(34691..35068)
                     /locus_tag="ACHJWA_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO4307743.1"
                     /translation="MKWFAAIAVVGALAGCARTAPIDQVHSTVSAGHTQEQVKKAILK
                     AGVERKWIMSEAGQGIIKARQQSRDHSAEIRINYTASSYDINYENSQNLQASGGQIHK
                     NYNRWVRNLDKDIQLNLSAGADL"
     gene            complement(35460..36509)
                     /gene="yhhT"
                     /locus_tag="ACHJWA_001098"
     CDS             complement(35460..36509)
                     /gene="yhhT"
                     /locus_tag="ACHJWA_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO4307744.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            36641..37858
                     /gene="yhhS"
                     /locus_tag="ACHJWA_001099"
     CDS             36641..37858
                     /gene="yhhS"
                     /locus_tag="ACHJWA_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4307745.1"
                     /translation="MPEPVAEPALNGLRLNLRILSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDVKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(37862..38419)
                     /gene="dcrB"
                     /locus_tag="ACHJWA_001100"
     CDS             complement(37862..38419)
                     /gene="dcrB"
                     /locus_tag="ACHJWA_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFO4307746.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(38492..39157)
                     /gene="yhhQ"
                     /locus_tag="ACHJWA_001101"
     CDS             complement(38492..39157)
                     /gene="yhhQ"
                     /locus_tag="ACHJWA_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFO4307747.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            39378..39623
                     /gene="tusA"
                     /locus_tag="ACHJWA_001102"
     CDS             39378..39623
                     /gene="tusA"
                     /locus_tag="ACHJWA_001102"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFO4307748.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(39725..41923)
                     /gene="zntA"
                     /locus_tag="ACHJWA_001103"
     CDS             complement(39725..41923)
                     /gene="zntA"
                     /locus_tag="ACHJWA_001103"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFO4307749.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFATSWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAALAIPAAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41997..42623)
                     /gene="yhhN"
                     /locus_tag="ACHJWA_001104"
     CDS             complement(41997..42623)
                     /gene="yhhN"
                     /locus_tag="ACHJWA_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFO4307750.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            42764..43123
                     /gene="yhhM"
                     /locus_tag="ACHJWA_001105"
     CDS             42764..43123
                     /gene="yhhM"
                     /locus_tag="ACHJWA_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFO4307751.1"
                     /translation="MSKPPLFFIVIIGLIIVAASFRFMQQRREKADNDMAPLQQKLVL
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(43126..43395)
                     /gene="yhhL"
                     /locus_tag="ACHJWA_001106"
     CDS             complement(43126..43395)
                     /gene="yhhL"
                     /locus_tag="ACHJWA_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFO4307752.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(43385..43981)
                     /gene="rsmD"
                     /locus_tag="ACHJWA_001107"
     CDS             complement(43385..43981)
                     /gene="rsmD"
                     /locus_tag="ACHJWA_001107"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFO4307753.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADETLIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            44131..45627
                     /gene="ftsY"
                     /locus_tag="ACHJWA_001108"
     CDS             44131..45627
                     /gene="ftsY"
                     /locus_tag="ACHJWA_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFO4307754.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQTVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAENEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            45630..46298
                     /gene="ftsE"
                     /locus_tag="ACHJWA_001109"
     CDS             45630..46298
                     /gene="ftsE"
                     /locus_tag="ACHJWA_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFO4307755.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            46291..47349
                     /gene="ftsX"
                     /locus_tag="ACHJWA_001110"
     CDS             46291..47349
                     /gene="ftsX"
                     /locus_tag="ACHJWA_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFO4307756.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            47594..48448
                     /gene="rpoH"
                     /locus_tag="ACHJWA_001111"
     CDS             47594..48448
                     /gene="rpoH"
                     /locus_tag="ACHJWA_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFO4307757.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            48719..49822
                     /gene="livJ"
                     /locus_tag="ACHJWA_001112"
     CDS             48719..49822
                     /gene="livJ"
                     /locus_tag="ACHJWA_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFO4307758.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            49972..50238
                     /gene="ataR"
                     /locus_tag="ACHJWA_001113"
     CDS             49972..50238
                     /gene="ataR"
                     /locus_tag="ACHJWA_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin AtaR"
                     /protein_id="HFO4307759.1"
                     /translation="MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRA
                     AEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKRAAKRLQGM"
     gene            50245..50772
                     /gene="ataT"
                     /locus_tag="ACHJWA_001114"
     CDS             50245..50772
                     /gene="ataT"
                     /locus_tag="ACHJWA_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin
                     acetyltransferase AtaT"
                     /protein_id="HFO4307760.1"
                     /translation="MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKIL
                     RAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQKKIPYKNIPSVTLGRLAIDRSL
                     QGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
                     FFPTKSIELLFTQSD"
     gene            complement(50769..51152)
                     /gene="panM"
                     /locus_tag="ACHJWA_001115"
     CDS             complement(50769..51152)
                     /gene="panM"
                     /locus_tag="ACHJWA_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFO4307761.1"
                     /translation="MKLTIIRLEKFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            51576..52685
                     /gene="livK"
                     /locus_tag="ACHJWA_001116"
     CDS             51576..52685
                     /gene="livK"
                     /locus_tag="ACHJWA_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFO4307762.1"
                     /translation="MKRNAKTIIAGMIALAISHTALADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            52733..53659
                     /gene="livH"
                     /locus_tag="ACHJWA_001117"
     CDS             52733..53659
                     /gene="livH"
                     /locus_tag="ACHJWA_001117"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFO4307763.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            53656..54933
                     /gene="livM"
                     /locus_tag="ACHJWA_001118"
     CDS             53656..54933
                     /gene="livM"
                     /locus_tag="ACHJWA_001118"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFO4307764.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            54930..55697
                     /gene="livG"
                     /locus_tag="ACHJWA_001119"
     CDS             54930..55697
                     /gene="livG"
                     /locus_tag="ACHJWA_001119"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFO4307765.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            55699..56412
                     /gene="livF"
                     /locus_tag="ACHJWA_001120"
     CDS             55699..56412
                     /gene="livF"
                     /locus_tag="ACHJWA_001120"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFO4307766.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            56531..57277
                     /locus_tag="ACHJWA_001121"
     CDS             56531..57277
                     /locus_tag="ACHJWA_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO4307767.1"
                     /translation="MHYAVVVMLELLCCIHAYRTGQERYWIFIIFCFPVIGCVAYFVI
                     VMLPETGADRHGHTLLMRLQDKLNPERHLRKLTEELAIAETNQNHYALANELARLGRY
                     HEAVPHYQQALSGIFAHEAAMMLSLAQAQFAIQEFAACQQTLEDVMRYNPDFQSADGH
                     LLFARTLAAQEKYADAESEFEVLISYYPGPQARIYYAEMLAKMSRLREANEQYVAVVD
                     TAKRSRPHYRKHHREWIKTANERLKQSVVQ"
     gene            57637..58953
                     /gene="ugpB"
                     /locus_tag="ACHJWA_001122"
     CDS             57637..58953
                     /gene="ugpB"
                     /locus_tag="ACHJWA_001122"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFO4307768.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            59051..59938
                     /gene="ugpA"
                     /locus_tag="ACHJWA_001123"
     CDS             59051..59938
                     /gene="ugpA"
                     /locus_tag="ACHJWA_001123"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFO4307769.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSRFVGLDNFVALFHDSYYIDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVMQFRYVESKVRYQ"
     gene            59935..60780
                     /gene="ugpE"
                     /locus_tag="ACHJWA_001124"
     CDS             59935..60780
                     /gene="ugpE"
                     /locus_tag="ACHJWA_001124"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFO4307770.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            60782..61852
                     /gene="ugpC"
                     /locus_tag="ACHJWA_001125"
     CDS             60782..61852
                     /gene="ugpC"
                     /locus_tag="ACHJWA_001125"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFO4307771.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDICINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIALPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLPENQLHLFDGETGQRV"
     gene            61849..62592
                     /gene="ugpQ"
                     /locus_tag="ACHJWA_001126"
     CDS             61849..62592
                     /gene="ugpQ"
                     /locus_tag="ACHJWA_001126"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO4307772.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYGKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARELWAGMTPPLLSSFEIDALEAAQQAV
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(62579..63019)
                     /gene="yhhA"
                     /locus_tag="ACHJWA_001127"
     CDS             complement(62579..63019)
                     /gene="yhhA"
                     /locus_tag="ACHJWA_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFO4307773.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPGTPQPDIPLKTIGP"
     gene            63139..64884
                     /gene="ggt"
                     /locus_tag="ACHJWA_001128"
     CDS             63139..64884
                     /gene="ggt"
                     /locus_tag="ACHJWA_001128"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFO4307774.1"
                     /translation="MIKPTFLRRVAIAALFTGSCFSTVAAPPAPSPVSYGVEEDVFHP
                     VRAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLI
                     RSKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALD
                     KYGTMPLNKVVQPAFKLARDGFIVNDELADDLKTYGSEVLPNHENSKAIFWKEGEPLK
                     KGDKLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVER
                     TPISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYA
                     YADRSEYLGDPDFVKVPWQALTNKAYAKSIAEQIDINKAKPSSEIRPGKLAPYESNQT
                     THYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGG
                     DANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSLIITTVLQMVVNSIDYGMNVAE
                     ATNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELY
                     GASDPRSVDDLTAGY"
BASE COUNT        15390 a        16814 c        17743 g        15142 t
ORIGIN      
        1 ccatctaatt agaagcaata ctatggcacc cacacctgta tatgctgatt atttaaataa
       61 cactactgat ttcagaccag atcctgaaga tatcttcaaa tgtggacaga cgctcgaatg
      121 tggcgacccc cgcaaaaaca ttagacaaaa atatggccat catgggttag gggtaaatca
      181 gaatcaacaa tttgataatg gtgttatggg gctaaattcc aggatggcag tgtttgatga
      241 aaataaaaaa ataataggtt cttcctcagg actaagttgt tttcaaccac attaaaactc
      301 tatgagtgct gtggtatatc tttttaatcc tgccagtatt tcatagtttt tcttaatcga
      361 ttgtagaggc atgtactgat gacgcctcta caatctttag ggactttctc tactatgaga
      421 taagaaattt catattatgc tttaaggctg aatgatttgt gcaaaatata attatttttc
      481 catcgaacat gaggtgtatg tcataagatt aaatgataac tatgtattcc tcgcggtgaa
      541 ttatatgagg taaaagatga aaattggaac tgtggcaggt actaacgaca gtacaacgac
      601 aatcgcaaca aatgatatgg tgcaagagca tgttactaac ttcactaaag aactgtttgg
      661 gtatattgca aatggtatag gtgatgatat atcaagtatt gccaggacaa tgctcggtga
      721 ggttctggaa aaagtagatg attggcaaat agaacggttc caacaatcta ttcaagatga
      781 caaaatttca tttaccatac aaacggatta ttcagaaaaa tatagtatgc tatcgggcat
      841 gcgtgctcat attctacgta ggaataacca ttatcagttt attgtgacga ttaatagcaa
      901 aaattacggt tgccctcttg ataataccga tattaattgg tgcggcattg tttatttact
      961 taataatatg acagtaaatg ataatgctaa tgatgttgca gttactgagt cgtacaaacc
     1021 catctggaac tggaaaatat cgcaatacaa tgtatctgat attaaattcg aaacaattat
     1081 aaaaccccag ttcgctgaca gaatctattt ctccaattgt tcacctgttg atcccacaag
     1141 taccagacca acctattttg gtgataccga tggctcagtt ggtgcagttt tgtacgcgct
     1201 ttttgcaacg ggacatttgg gaatcatggc tgaaggagaa aatttcctta gccagttgct
     1261 taatatagaa gatgaagttc tcaatgtatt gctaagggag aactttaatg agcaagttga
     1321 tactaatgtt aatacaataa tcagtatcct gaacagaaga gacattattt tagaaagtct
     1381 tcagccttat ttagttatta ataaagacgc agtgacgcca tgtacgtttt tgggtgacca
     1441 aaccggcgat cgatttagta atatctgtgg cgatcaattt atcatcgatt tgttaaaacg
     1501 cataatgagc atcaatgata acgttcatgt attagctgga aatcatgaaa cgaattgcaa
     1561 tggtaactat atgcagaact tcacgcgtat gaaaccactt gatgaggata cctacgacgg
     1621 gataaaagat tatcctgtat gtttctatga ctccaaatac aaaataatgg caaatcatca
     1681 tgggataact tttgatgatc agcgaaaacg ttacattata ggaccgataa cggtctctat
     1741 tgatgaaatg accaatgctc tcgatccggt cgaattagcg gcaattatta ataaaaagca
     1801 tcatactata attaatagta aaaagtttaa aactagccgg gctatctctt gtcgatcctt
     1861 taatcgttat ttttctgttt cgacagatta cagacctaaa cttgaagcac tattagcatg
     1921 ctcacaaatg ttaggtataa atcaagttgt tgcacataac ggtaatggtg gaagagaatg
     1981 cataggtgaa acggggacag ttttaggtct taacgcccgt gacagtaaac atacgggaag
     2041 aatgttcagc atgcataatt gccaaattaa ccctagtgcg gggcctgaga taaccactcc
     2101 ctggaagtct taccaacatg aaaaaaacag aaatggactt atgcctttga ttagaaggcg
     2161 cacaatgtta cagctctgaa ccatagacaa catgaatatt aatgttacta tctggttgag
     2221 actgttgata aaacgtaata aggaatgctc tgttcctgca actgaacagg cgtgaaacgc
     2281 tatccaacag gatggatacc gttttgtcag ccagttttat agatcacact tatgagtatg
     2341 cgtgtttcat ttacggccag gaatgctgat gatgacaaca ttactacccg ttttcactaa
     2401 gccttccccg ttggcgctca atgctctgcg cgctggtcgt atttgtcgtt tccttcttat
     2461 cccggatgga agaatccgct gaacgtcgtt cggcggcagt tgtaacacgc gtcgtttgct
     2521 tacagggatt atggcgggtc gattatggat aagagtaagc gccatctggc gtggtggggt
     2581 gtcgggttac tggcggtggc tgctatcgta gcgtggtggc tgttgcgccc ggcaggtgtg
     2641 ccggaaggct ttgccgtcag caatgggcgc attgaagcga cggaagtgga tatcgccagc
     2701 aaaattgccg ggcgtatcga caccattctg gtgaaagaag gccagtttgt tcgcgaaggc
     2761 gaagtgctgg cgaagatgga tactcgcgtg ttgcaggagc agcgactgga agccatcgcg
     2821 caaatcaaag aggcacaaag cgccgttgcc gccgcgcagg ctttgctgga gcaacgacaa
     2881 agcgaaactc gcgccgcaca gtcgctggtt aatcaacgcc aggccgaact ggactccgtt
     2941 gccaaacgtc atacgcgttc ccgctcgctc gcccaacgag gggctatttc tgcgcaacag
     3001 ctggatgacg atcgcgccgc cgctgagagc gcccgagctg cgctggaatc agcaaaagcc
     3061 caggtttcgg cttctaaagc cgctatagaa gcggcacgca ccaatatcat tcaggcgcaa
     3121 acccgcgtcg aagccgcaca agccactgaa cggcgcattg ccgcagatat cgatgacagc
     3181 gaactgaaag ccccgcgtga cggacgcgtg cagtatcggg ttgccgagcc aggggaagtg
     3241 ctggcggcag gcggtcgggt gctgaatatg gtcgatctca gcgacgtcta tatgaccttc
     3301 ttcctgccaa ccgaacaggc gggcacgctg aaacttggcg gtgaagcccg gctgatcctc
     3361 gatgccgcgc cagatctgcg tattcctgca accatcagtt ttgtcgccag tgtcgcccag
     3421 ttcacgccaa aaaccgtcga aaccagcgat gaacggctga aactgatgtt ccgcgtcaaa
     3481 gcgcgtatcc caccggaatt actccagcag catctggaat atgtcaaaac gggcttgccg
     3541 ggcgtagcgt gggtgcgggt gaatgaagaa cttccgtggc ctgacgacct cgtggtgagg
     3601 ttgccgcaat gacgcatctg gaactggttc ccgtcccgcc tgtcgcgcaa ctggcgggcg
     3661 tgagccagca ttatggaaaa accgttgcgc tgaacaatat cacactcgat attccggccc
     3721 gctgtatggt cgggctgatt ggtccggatg gcgtcgggaa gtcgagttta ttgtcattga
     3781 tttccggtgc tcgtgtcatt gagcagggca atgtgatcgt gctgggcggc gatatgcgcg
     3841 acccgaagca tcgccgcgac gtctgcccgc gcatcgcctg gatgccgcag gggctgggca
     3901 aaaacctcta ccacaccttg tcggtgtatg aaaacgtcga ttttttcgct cgcctgttcg
     3961 gtcacgacaa agcggagcgg gaagtgcgaa tcaatgagct gctgaccagc accgggttag
     4021 caccgtttcg cgatcgtccg gcagggaaac tctccggcgg gatgaagcaa aaactggggc
     4081 tgtgctgtgc gttaatccac gacccggaac tgttgattct tgatgagcct acgacggggg
     4141 ttgacccgct ctcccgcgcc cagttctggg atctgatcga cagtattcgc cagcggcaga
     4201 gcaatatgag cgttctggtc gccacggcct atatggaaga ggcggaacgc ttcgactggt
     4261 tggtggcgat gaatgccgga gaagtgttgg caactggcag cgccgaagag ctgcggcagc
     4321 aaacgcaaag cgccacgctg gaagaagcat ttataaatct gttaccgcaa gcgcaacgcc
     4381 aggcgcatca ggcggtagtg atcccaccat atcaacctga aaacgcagag attgccatcg
     4441 aagcgcgcga tctgaccatg cgttttggtt ccttcgttgc cgttgatcac gtcaatttcc
     4501 gcattccacg cggggagatt tttggttttc ttggttcgaa cggctgcggt aaatccacca
     4561 ccatgaaaat gcttaccgga ttgctgcccg ccagcgaagg tgaggcgtgg ctgttcgggc
     4621 aaccggttga tccaaaagat atcgatactc gccgtcgggt gggctatatg tcgcaggcgt
     4681 tttcgctcta taacgagctc accgtgcggc aaaaccttga gttacatgcc cgtttgtttc
     4741 acatcccgga agcggaaatt cccgcaagag tggctgaaat gagcgagcgt tttaagctca
     4801 acgacgttga agatgttctg ccggagtcat tgccgctcgg cattcgccag cggctttcgc
     4861 tggcggtggc ggtgattcat cgcccggaaa tgttaatcct cgatgaacct acttctggcg
     4921 tcgatccggt ggtgagggat atgttctggc agttgatggt cgatctctcg cgccaggaca
     4981 aagtgactat cttcatctcc acccacttta tgaacgaagc ggaacgctgc gaccgtatct
     5041 ctctgatgca cgccggaaaa gtgctcgcca gcggtacacc gcaggaactg gttgagaaac
     5101 gcggagccgc cagtctggaa gaggcattta tcgcctattt gcaggaagcg gcaggacaga
     5161 gcaacgaagc cgaagcgcct cccgtgatac acgacaccac acacgcgccg cgccagggat
     5221 ttagcctgcg ccgtctgttt agctacagcc gccgcgaagc gctggaactg cgacgcgatc
     5281 cggtacgttc gacgctggcg ctgatgggaa cagtgatcct gatgctgata atgggttacg
     5341 gcatcagtat ggatgtggaa aacctgcgct ttgcggtgct tgaccgcgac cagaccgtca
     5401 gtagccaggc gtggacgctc aatctctccg gttcccgtta ctttatcgaa cagccgctgc
     5461 ttaccagtta tgacgagctt gatcgtcgga tgcgtgcggg cgatatcacg gtggcaattg
     5521 agatcccgcc caatttcggg cgcgatatcg cgcgtggtac gcctgtggaa ctcggtgtct
     5581 ggatcgacgg tgcgatgccg agccgcgccg aaactgtaaa aggttacgtg caggccatgc
     5641 accagagctg gctacaggat gtggcgagcc gacaatccac accctccagc caaagcgggc
     5701 tgatgaatat tgagacgcgt tatcgctata acccggacgt aaaaagcctg ccagcgattg
     5761 ttccggcggt gatcccgctt ctgctgatga tgatcccgtc aatgctaagc gcccttagcg
     5821 tggtgcggga aaaagagcta gggtcgatta tcaaccttta cgtgaccccc accacccgta
     5881 gcgaattttt gcttggcaaa cagctgccgt acatcgcgct ggggatgctg aactttttcc
     5941 tgctctgcgg cctgtcggtg tttgtgtttg gcgtaccgca taaaggcagt ttcctgacgc
     6001 tcaccctggc ggcgctgctg tatatcatca tcgccaccgg aatggggctg ctgatctcca
     6061 cctttatgaa aagccagatt gccgccattt tcggtacggc gattatcacg ttgatcccgg
     6121 cgacacagtt ttccgggatg atcgatccgg tagcttcgct ggaagggccg ggacgttgga
     6181 tcggcgaggt ttacccgacc agtcattttc tgactatcgc ccgcgggacg ttctcgaaag
     6241 cgctggatct gactgatttg tggcaacttt ttatcccgtt gctgatagcc ataccgctgg
     6301 tgatgggctt aagcatcctg ctgctgaaaa aacaggaggg atgatgcgcc atttacgcaa
     6361 tatttttaat ctgggtatca aagagttgcg cagtctactc ggtgataaag cgatgctgac
     6421 attgattgtc ttctcgttta cggtgtcggt gtattcgtca gcgaccgtca cgccaggatc
     6481 gttgaacctg gcaccgatcg ccattgccga tatggatcaa tcgcagttat cgaaccggat
     6541 cgttaacagc ttctatcgcc cgtggttttt gccaccggag atgatcaccg ccgatgagat
     6601 ggatgccgga ctggacgccg ggcgttatac ctttgcgata aatattccgc ctaattttca
     6661 gcgtgatgtc ctcgccggac gccagccaga tattcaggtg aacgtcgatg ccacgcgcat
     6721 gagccaggca tttaccggca atgggtatat ccagaatatt atcaacggtg aagtgaacag
     6781 ctttgtcgcg cgctaccgtg ataacagcga accgttggta tcgctggaaa cccggatgcg
     6841 ctttaacccg aaccttgatc ccgcgtggtt tggcggggtg atggcgatca tcaacaacat
     6901 taccatgctg gcgattgtat tgaccggatc ggcgctgatc cgcgagcgtg aacacggcac
     6961 ggtggaacac ttactggtga tgccgataac gccgtttgag atcatgatgg cgaagatctg
     7021 gtcgatgggg ttggtggtgc tggtggtatc gggattatca ctggtgctga tggtaaaagg
     7081 cgtgttgggc gtaccgattg aaggctcgat cccgctgttt atgctgggcg tggcgcttag
     7141 tctgtttgcc accacgtcaa tcggcatttt tatggggaca atagcgcgtt caatgccgca
     7201 actggggctg ctggtgattc tggtgctgct gccgctgcaa atgctttccg gtggttccac
     7261 gccgcgcgaa agtatgccgc agatggtgca ggacattatg ctgatcatgc cgacgacaca
     7321 ctttgttagc ctcgcgcagg ccattctcta ccgtggtgcc ggattcgaaa tcgtctggcc
     7381 gcagttcctg acgctgatgg caattggcgg cgcgtttttc accattgcgc tgctgcgatt
     7441 caggaagacg attgggacaa tggcgtaagg aagggaaact tgacttcata ctgtgatgtc
     7501 atactattga tgtcataaag tgaagtcata aggacatgcc atgagaacca aggtacaggc
     7561 tttacggaag aaacaaaaaa atacattgga tcaaatattt aaaactcctg ttcctcaagg
     7621 gatcaagtgg tctgatatag agtcactggt taaagcatta ggcggagaaa ttaaggaagg
     7681 aagaggttcg cgttgtaagt tcatactaaa tatgagcgtt gcgtgtttcc atcggcctca
     7741 tccgtcgcca gataccgata aaggcgctgt agaaagcgtg cgtgactggt tactaagtat
     7801 aggggtaaaa ccatgatcaa actgaagacg ccaaactcta tggagattgc cgggcaacct
     7861 gctgttatca cttacgtgcc agaactgaat gcatttcgtg gcaaattcct gggcttgtca
     7921 ggttattgtg attttgtctc tgacagcatt caggggctgc aaaaagaagg ggaactttcc
     7981 ctgcgagagt atcttgagga ttgtaaagct gcgggtattg agccgtatgc gcgtacagaa
     8041 aagattaaga cctttacgtt acgctatcca gaatcgctga gtgaacgcct taataatgcg
     8101 gctgcgcagc aacaagtctc tgttaatacc tatattattg aaacactgaa tgagcgtttg
     8161 aatcatttat aacttatagc ggcccaacaa tttctgttgc gccgctatat ttttgtggct
     8221 cttaagatgc ctttctaacc aaccgatgcc cctcattcat catcaggatt ttgctataca
     8281 catcttcctc gacggcgttt tttgctgatg ccatgactcg tgccagattc gcctcgttgt
     8341 gattattcac atacgctttg ccgacggcgg tgataaacgc tttgcgcaga tcgctggcga
     8401 tatttacttt agcgacgcga taattaacaa agctgcgtaa tatctccggg gcgataccgg
     8461 agccgccgtg aataaccaac ggcaccggac agacttcggc aatgcgcttc agcaggggga
     8521 tatcgatacg cggtatatcg tccaggccat gaacgttgcc aatagaaact gccagcatgt
     8581 cgcaaccggt acgctcgaca aaggtcttca ccttttccgg ttccgttttg caatcggctt
     8641 cgctgacgtg atcatcttct ttgccgagaa tcgcccccag ctccgcttca accggtacgc
     8701 cataggattt acagaaatcg acagcttcct gggtgaaagc gatattctct tcaaaagaga
     8761 atgcggcacc gtcaatcatg accgaggtaa agcccgcgcg caccgcctgt ttaacatcct
     8821 cctgggtttt cccgtgatcc agatgaagac tgaccggaac atccatttta tcggcgtgac
     8881 gtttcacgat ttcataaatg taatcgtatc ccgagagttg tgcattggtt ggcgcaatct
     8941 gaatgaagtt gggtaatccc gaacgctcaa tagcgtttaa aattgaaatt gtcgtctcca
     9001 gattggtggt attaaatgcg cccgctaata cccgtttttc ctgaatgcgg tcgagcagta
     9061 tccgtccatt aactaatggc atattgaaaa cctcatcttt taatatcagt taattattca
     9121 gcttcaccga agcggtcgcg aaataagtcg gtgagagtct gtatggcctc gttttcatca
     9181 tcaccgtcgc agcgtatttc caccgttttt ccgcctttta ttccggcgcg cataatattc
     9241 atcatgctgg tggcattaat atcattaccc tcatagtgca gcgtcacttt acagctatat
     9301 tttttggcgg ctttcgccag acaggccgcc ggacgtgcat gcaggccagt actgtttctg
     9361 acctccacgg ttttattcaa catagtgttc ctctcgatga tgtaatggca tcacgccttc
     9421 ttgttgtaat ttcaactgat accagctttt ccatttgtgc tgataacgct gtaattgatg
     9481 gtatctcgcc cgattcggta acaaacgttg ggctatagcg actggcggcg gtggaatatc
     9541 cggtgcggcg gttaataatg ctccgacaga aaccgcctcg ttaaatgcaa ttgcttcaac
     9601 cggacgctgt aaaatatccg cctttaattg cagccaaaga ggattatggg ttgccggacc
     9661 gatcactttt aacgcgctgg tgccaggcac gttaaaacag gcgaagcagt tggcaaattc
     9721 catggttaat cccagtgata cgctggcaat taacatgtcg atacttaacg tatcgccaag
     9781 cccataaaat aatccacggg tatggcgatt tttatagggc gaacccgaac cgcgaagatg
     9841 tggaataaag acgggaatgt catcgagcaa ccaattcccc gccagataat cagcatgtcc
     9901 gcgagtaagt gatgcggcga tctcctcatc ggttagccgg aacgtgttgc gcagccactc
     9961 cagcgcgaaa ccgcccacgg gcagcgacgc aaacagggtg aactggttgg caagtgagta
    10021 acagccgttt gccagcttgt cacgtttggc ctgttcatcc agcgtcggtt gtgtatccag
    10081 ttgcagaatg ccttccgtgg tccccgtcga gttaaggata tcgcctggca tcatctgcaa
    10141 cgctcgcgca cccaccatat ggtcatgtcc ggccagcgtc acgctgaccg gttgcgagaa
    10201 cccaagcgtc ttgcaaagtg actcgctgac ccatccggcg tgctcgcctg gctgcaccaa
    10261 tggcgcaaat gccgtacagg gaacgtgtaa caacgccgcc gctttcgctg accattcgcc
    10321 tttgcgcacg tccagacaca gcgtgcggct ggctaaggtg atatccgtgt gctgttcgcc
    10381 agtcagccgc cagagcagca cttccggcgc atgtagccag cgcaggccgc ggcgattacg
    10441 cagcgggtaa tgttccagca accagcgcat tttgaaggca gagtaattgc tgtgtagtgg
    10501 tagaccgcag atgtcataaa gtgccgcact gtctgcctcg ctaagcgttg ccagatactc
    10561 ttcaccgcga cggtcatacc atgccagcat tggcgtcagg atctcgccat gctcgtcaag
    10621 gaacacgcct gattcgccaa aactggcaat gctgatccgt ctgactggca gcggcgaagc
    10681 ggcgttcagt tgcgctatcg cctggcggac ctcctgccag agggcgtcga tatcgaaatc
    10741 gacatcgccc tgtggggaga tctgctttgc cgtcacgaat ttatgcgcgc ccaacgtcgt
    10801 tgcgtccagg caggaaaagc aggtgacttt gcaattggtg gtgccaatat cgataacgat
    10861 agctgcgctg ttatcaggca taaaccgtgc tccgcgtcat tcggaaaatg ccatacagta
    10921 ccagggcgat aaccggcacc agaccaatgg cgatgagatt gccgttgaag atttccagca
    10981 gccagaagcg gaacgggtta ccgccatcaa gaaaactgga aacctggccg ctggagcctt
    11041 cggcaaattt gaagttaacg tctttcgcca ttccggtaat aaacggcgcg attttggtag
    11101 caatccatag gtcggcaata atcaccggga tcgccgccag aacggtgcgg aaaatattgc
    11161 cacggctggc taaagcaatc atcgacgcca ttaccgccag gttggcaagg tcgccaagcg
    11221 gcaggacttc attacccgga aggacaaacg cgataaagac cgagattggc gttagcagca
    11281 gggcggtgga gataatcgcc ggattaccta cggcaacggc gatatccagg ccgatataga
    11341 gatcgtcacg gtcagggtat ttggcattga gatacttttt aatagcttca gacagcggta
    11401 ataaaccttc catcaggatg cgtaccatgc gtggcaggat gaacatcaca ccgccaatgc
    11461 tgatgccaag cagcaatact tttttgaaat cgtatccggc aattacgccg agcagaatgc
    11521 ccagaatagt gccaaccatc atcggttcgc cgaagatgcc aaactttttc tgtacggttt
    11581 ccgggtcgat atgaatacga ttgaggccag ggatatggtc gatgattttg tcgaccagca
    11641 gaccgaccgg gaagaacacc accgaagaga gcgtcggcaa tgagatccct tccagtccaa
    11701 agtatttttg taccagtggc gcagaccagt cggcgagttt aagtactacc accgcagcaa
    11761 tcgccgccgc cagcaaacca aagaacaggc tgccggtgct gtaataaacc aacgtaccag
    11821 caagggcaaa atgccagtag ttccacatat ctacgtcaac ggtacgggtt ttatttaacg
    11881 ccagcatcgc cacgttaatc agaatggtca ggggaataac aaacggggca attggcgaac
    11941 cccatgtaat agcggaaagc ggcggccagc ctaaatctac caccggtaaa ttaattccgg
    12001 tgcgttcaac catcgcctgg gccgctggcc cgacattgct ggtcagaata tcgaaaatgg
    12061 cataaatacc gacaaaacca atgccgactg ttaatgacga acgtaaggct ttagcgggtt
    12121 ttactttaaa acagagagca agaataaata acaccaacgg caacattacc gtcgggccaa
    12181 atcccaatat atattgcaca atattataga gggtatgcgc gatatcgttc ataataaacc
    12241 tctttttcag gcgagagcgc acctcgcgtt agcgcctgat ggagtgggtt gaaattaatt
    12301 attattaatt actttttaag gatagaaaca atttgcgcca gtactttttc ttcgccaata
    12361 ccggtaataa tcggcaagcc gttcaccacc gggacgtcga gttcataagg cactttagtt
    12421 gttgaaacta ccagtgctac gccgtcagta tttccaggta aagaggcaac gttggtttgg
    12481 gaataattga cgttaagacc ttgctctttg caatattcca tgactttttc tgttaccgcc
    12541 gtggatgtgg caatacccgt ggcgcagaca aataaaatgg tttgactcat atttcacctc
    12601 gttctttcat tttgtttaag attactgtgt gtatttctgt tgtttccatc gcaagcaggg
    12661 cttgcataaa gggcgtatcc tgaatcattt ccataatcca gcccagggca tttaattgtt
    12721 tattgctttc gcttaaggcg agcaaaaaaa tcaccctgac aggtacgggg tcaggatcgc
    12781 cgcccatatc ggcaaactct accggttccg gcaggatgcc gacggcaatt gcattgtgcc
    12841 tgacatgttt atggtccgtg tgcggaatag cgacgcacgg tggttcggta ggcagccccg
    12901 ttgggtacgt cgcctcacgt ttcagtacgt gatcggcaaa atcagggacg accagctcgg
    12961 cggcaaccaa cggttgcgct aactggcgga tgatctgttc aacgcttaac cctgccggaa
    13021 gtcgttgaat ataattttcc tgaaaatgta tgccttgcat tttattcctc ttaaaaaagt
    13081 gtacttaact cacttcccgc cgttgcagta cggtgccaag ataatattta tttcctttta
    13141 accagacgtt gccaaactca atgggtaatc ctcgggagaa aaagactaac tgctccagat
    13201 gcaaaacggg tgcgtcttca ctgatatcta aaaaatgacc acgttcatta ccaattaatc
    13261 gcgcggcata gcgactttcc gagtaacgaa tttttctttg cgacaaactt tctattgttg
    13321 gaaataaatt gtgttgatta aaatcgattt cagcaatacc ggggcatagc tcaatattga
    13381 tacggttctc tatcagcatc gctttttcat caccaattga gcgtaagcgt tcaaggtaaa
    13441 gaatatcctg accgggcgtt attcttaatt tctctgccac ataacgatta gccgattcaa
    13501 tccgtgacgt aataacttca gtggtaaaat gtattttctg gctttccagc gattccgcga
    13561 atgacaataa tccttcacct aacggatatg ccacgttctc ttttttgaca aatgttccct
    13621 taccctggac ctgtattaac acgccttctt ttaccagttg ggcgaccgct tttttaacgg
    13681 ttccccgact cacgtccagc atatccataa tctggtattc cgaaggaatg cgatcgtctt
    13741 ccaccagatc tccggtataa atactttctc ttatccactc caccacttgt tgatagagcg
    13801 gtatttgtga cgacttactt aatgatttca tggcgcgcac ctgaacattt acagttgtct
    13861 atggacaatc atggacaact tcaagcgtat agtaggtgaa cgggagaaaa agaggatcga
    13921 agcgtctcac attttgaggg accaaatcgt gatcaaaaaa atgtgacgtg agtcgatatt
    13981 tgtcagaagt ttactgcgcc gtaaggaaat aacggcgcgt tggtaggtgt taatcttcct
    14041 tcggcaggca ctgcaaatgc ccgtgccgca cgccgcgctg ggcgataacg tcatcggcaa
    14101 aatgctgcac gtcacccatg tcacctttca acacggcgat ttccagacag tcgtcgtggt
    14161 tgatgtgcac atgtagcgtg gcgacggaga ggtcgtggtg atgatgctgg gtggaaacaa
    14221 tacggctggc taagtcgcgt ttttcgtgtt catacacata cgacagcacc gcgaaacctt
    14281 gtgtgccgtg ctgctgggta gcttcctgcg ccagggcgct acgcagaata tcgcggatag
    14341 cttcggaacg gttgttataa ccacgacgct ggctcaggct gtccagcgtc tccagtaaat
    14401 cgtcatcaag cgtgatggtg actcgttgca tttgcgttaa accttttctg tggcgcgacg
    14461 gcgcacgggg aatgcgggta atatcgcgtt ttgtagcaca cgtccggcgt cagaggaaaa
    14521 ggttaatttc tctcccaccg cctgggtttc gacgatttgt ccgttgtcca tcaccatcac
    14581 tcgctggcaa aagcgttcca ccaggcgtaa gtcatgagtg atgaacaggc aggcagtgcc
    14641 aaactgttgt tgtagctttt tcagcaggcg aatgacgccc gcctgtaaca cgagatcaag
    14701 gttagaaacg gcttcatcca gaatcagcaa tttcggttcg accgccagcg cgcgtgccag
    14761 gcagacgcgc tggagctggc cgccgcttaa ctgcggtggg cgcttgtcga gcacgctgtc
    14821 gtcgagatcg accgcgtgca gcatttcgct ggcacgcgcc agttgttcgg attttttcag
    14881 cgatagcagg tggcgcatcg gttcccgcag gatctcgcgc acggttttgc gcggattcac
    14941 ggcgctgatg gagtcctgaa ataccatctg aatatcgcgg cggaacgctt tacgctgggc
    15001 gcgattgagc tttgccaacg attcgccacg ccagctaata tttccctgtg agggcgattc
    15061 taagcctacc agtagccgcg agagagtgct ttttccgcag ccgctgcgtc ccagcagggc
    15121 gacggtttca ccgcttttca gggtcaggga aacgttattc agcaccgcct ggtgctgatg
    15181 ttttccgcta aacccaccgt gggcatagtg atgggaaagg tcggagacgt taagtaaagt
    15241 catgatgcca gctccatacc gtagagggcg agatgagcgg aaaccaggct gcgcgttacc
    15301 gcatgtttgg gggcgttaaa cagcgtttcg acatcgccct gttcgacaat cttaccgtca
    15361 gacattaccg ctacgtcatc cgccagacgc gccaccacgc ccatatcatg ggtgaccagc
    15421 agcatccccg gcgcctgttt ttgcataatg ctttccagca gatcgagaat gcgtgcctgt
    15481 gctaccacgt cgaggtcggt ggttggttca tcggcgatga taaacggtga ttcgcaaagc
    15541 accgccatcg caatcatcat gcgctgcaac atgccgccgc tcatctcgaa cgggtacagc
    15601 ttcagcacgc gtgcggcgtt ttccagcccc accgcttcta tggcagcggt aagcgtagcg
    15661 tcatcggcgg gtttccccag cgccaggcac gtctcccgcg cgtgggtgtg catggtgtgc
    15721 agtggattaa aggcgctgcg cgggttctgc atgatggtgg caattttgat gccgcgcagg
    15781 gcgcagggag aaaccggttt gccatcggct aaaatttccc ccgccgtctg gcgaacgcct
    15841 gcgggcagaa ttcccagcgt cgcggcgcag gttaatgatt tcccgctgcc gctaccgccg
    15901 actaacgcca gcacgcgccc gcgtttcagg gttaacgaca cgccgtgcac cagcggctgc
    15961 gcggcctgta gcgcgatatt acgtagttca atctgttgcg gcattagtgt gcgtgctccg
    16021 tcaccagatg aggatccaga tgatcgcgca gtgcgtcacc caccaggtta aaggccatca
    16081 cgctgataaa cagcgccagc cccggccaga acatttgcag cggctgggtc cagatatact
    16141 ggcgcgcgtc gttaatcatc acgccccatt cggcggtcgg cgcggtcacg ccgaggccga
    16201 ggaaggacat cccggcaacg tgcagcatca tatggccgat atccagcgtt gccagcacca
    16261 gcagcgaagg gatcaccgca cctgccagat gatcaacaaa cactcgcaca tggcccgcgc
    16321 cagaaagccg tgatgccagc acaaactcgc gctggcgcag tgaaatcacc aggctgcgca
    16381 ccatgcgtgc ataccacgcc cagtgcgaca gggcgatggc gataattacg ttggtcagcc
    16441 cagtgccgag cacgccaacc ataaagaatg acagaatcga ggtcgggaag gtcataaaca
    16501 tatcggcgac gcgcatggta gcctgatcaa cgcgcccgcc aatcagcccg gcgctgccgc
    16561 caataaccag ccctaatgtc agcaccagca gcaggcaggc cattaccgaa ccgagcgaca
    16621 cacgggtcgc tgccatcagc cgcgagaaaa tatcgcgccc taagtgatcg gtgcccaacc
    16681 agtgctgcgc atccggcgaa agcaggcgcg acggcaaatc aatcgcctgg gggtcatacg
    16741 gcagccacca ctggctggtg agcgcaatca gcgccagcag ggcgataatg atcagcgcca
    16801 ggcgtaccga ccagcgggaa gagaggaaaa agttcacgcg tgcgctcctt catgacgacg
    16861 aatgcgtggg tccagtgcgg cgttgagcaa atcgacaatc aaattacaga ccacaaaaac
    16921 caccaccatc atcagcgtaa agcactggat caccggatag tcacggttaa aaatcgccga
    16981 caccgcatag cgcccgacgc cgggccaggt aaagatgttt tcgataatca tcgtcccgcc
    17041 aatcagttcg ccaatgtgca tccccacggc ggtgatcatc ggcagcgagg cattgcgcag
    17101 aatatgacga cgctcggtct gtttgtcatt caggccgcgt agccgcgccc aggtgacgtg
    17161 acgctgaccg gcgacgtcca gcatactggc gcgcagcagc cgtgcgttaa tcgccagcga
    17221 cataaaggca atggaaaccg caggcaaaat gatgtgctgc cagccgccgt agcccatcgc
    17281 gggtagccat tgcagataca ccgaaaacgc catcaccagt aaaaatgcca gccagaagtt
    17341 gggcatcgac acgccgagaa acgcgatgaa acgtacggcg aaatccggca gacggtcacg
    17401 atggcgcgcc gcccagatac cgagcggtac ggaagtgagc agaattaata ccagcgccgc
    17461 acccgccagt tccagcgtgg cgggcaggaa gttcagcata tcgtccagca ccgggcgctg
    17521 cgtggcaaat gagatgccga aatcaagatg cagcgccttc cacaaccagg tgccgtactg
    17581 cacatacagc ggctgatcca gtcccagcat ggtgcgggta gaggccagca tctccggcgt
    17641 cggcggcagg ttagacagac gcaaataatc gagcgccggg tcgccggtac cgaggcgcag
    17701 catcagaaaa atgatcaccg aggcggcgag caccatcggg atcagcagca gaaagcggcg
    17761 taatacgtaa cgcaacatta aggtttcacc ggtttaatct gttcgaacgg aatttcggtg
    17821 gcgatcggcg cgtaggggat gttacccagc tccggttttg ataccaccat cattgagatg
    17881 taactgatag gcagataaac cgcctcgtca tgcagacggg tcagaatgtc gcgatacagt
    17941 gcctgacgtt gtgcttcgtc atgggtcgcc agcacttcgc cgatctcttt atcaatcagc
    18001 ggcttgtcgg ctaatccttg ctgtgcctgg aagtcagcgt gcgacggtac gcgcattgaa
    18061 ctcaggaagg cgtgtggatc gtatggcgcg ccccaggtgc ggtggaaaat catgccaaaa
    18121 cgaccgtcgc gctgacgcgc atagatactg ctctcttctt cgccaatcag cgagacatct
    18181 gcgccaatct ggcgcatatc agcctgaatg atttccgcca tcgatttgct taacgcatcg
    18241 gtgccgatga acgaaagttc aatgtgcagc ggctgaccat ttttctcgcg gatgtctttg
    18301 cccgcaggca gcgtccaacc ggctttttcc agcaacgctt tcgctttctg cggatcgtac
    18361 tggcgcggtt tcaggccgag gttggcgtag ggcacagaag gggcaaacag ggtgtcggcg
    18421 acctgctggg tgccatacaa cgcgttatca atcagcgatt ttttgtttac cgcgtaatta
    18481 agagcttcac gtactgccag ctcattggtg ggggctttgg cggtattaag cgccagcatc
    18541 acggtttcga tcggctgtga cagttgggta tggtaagccg ggttctggct aaagcgggcg
    18601 aaggtatcga gcggtaataa cccttcgttt ccgtacagca ggtcgatatc gccagtttca
    18661 aacgccaccg cgcgggtagt cgggtctggg atgacgttaa aagtgatctt cttaattgcc
    18721 ggcttttcgc cccagtagtt ttcgttacgg acgaagacat cgtactgatt cagtttcgat
    18781 tcctgcaaaa cccacggtcc ggtgccaatc ggcgctttaa tcccgttcat ggtttcatgg
    18841 tttttaaact gcgagggggc gataaagcgg aaagggcgcg gcagggccag ttcttgcagg
    18901 aaaggatagt aggcgctttt cagggtgatt tgcagctctg ttttactgag tgctttaaca
    18961 tcaacaatct ggtttgccag ctccagccag gcgtgacgtt gacggttatc gagcactgcg
    19021 cggaagtttt ctgccgccgc ctctgcatca aacggttcgc cgttagagaa tttcacgtca
    19081 tcacgcaggg tgaaggtcca ggttttacca tcttctgaat gagtccagct ttttgccagc
    19141 cacgggatca ccgaaccgtc tgcctgatat ttcaccaatg gttcataaac catgctctgg
    19201 gcgaacatct ggttaggcgt gtaaaggtgc gggtttagtg gcccgacatt caccggccag
    19261 gcggtggtga tttcatctgg tgcagcggca tggacgataa aagacgcaca agccagcagc
    19321 gcaaatagag tgcggcggag tgtggagagc atggtaaccc caatggatta aaatagatgg
    19381 cggaaataag tatgacgatt ttaagtattc gtcatactga ttacctgttc tggatcaata
    19441 gatgggcagt cgggtgggcg gatcagttaa ctgaatcgat ccattgcaca ctgtcggcgg
    19501 tgagcgtaaa gggagtaggg gtgcagatcg ccaggcttaa attttctagc tgacaatggc
    19561 tgactgacag cgaggagtga taggtgctgt ctacgctgac gatttgccag gcgctgccac
    19621 cacgctgttt aacgatggct tctttgcgcg tccagatgcg ccagaacatt tccagttgct
    19681 gatcaggatg cacggcgtcc atctcagcat gttccccgag gctgaacacg gcgttcgcca
    19741 gccagcgcca gttggcgcgc gggcgaatca cttcgatatc gcagccgact tcgccttcat
    19801 cactcaacag cagggcgata tcgtcaccgc tatggcttaa gttgaaccat agcggcgttt
    19861 ccggcgcaaa tgcaggtttg ccttgttcgc catagatgat ctccggtagc ggggaaagcg
    19921 tgtgcgaaag caatgcacgc cccgccagcc agcgttcgcg tcgtggacct tgcggtgctt
    19981 gctcgcgtaa acccggtggc agtggagctg cgcttaaggt cgaaactttc cccagaacta
    20041 tccgatacat atcagggcca acgtttaatg atgatggatg tattaatgcc gccaaaggca
    20101 aagttgttac tctgcaaaaa ctcacaatca accttgcggg cttcatgcat gatgtaatcc
    20161 agcgcgccac aattcgggtc tggtttattc aggtttagcg taggcgcaaa ccaaccttcg
    20221 cgcatcattt gcagactcat ccaggcttcg agcgcgccac aggcaccaag cgtatgacca
    20281 aaataacttt tcagcgagga gagcggcaca ttatcaccgt aaatggttgc tgtcgccaga
    20341 ctttctgcca tatcaccgcg atcggtcgcc gtaccatgcg ctgaaatata gccgatatct
    20401 tgtgcgctta acccggcgat tttcagcgat tgctccatac aatactgcat ggtttcgcgc
    20461 tgcggctggg taatatgtgc cgcgtcgcag ttagtcgcaa agccgacgat ttcaccgtaa
    20521 atggtcgcgc cgcgcgcttt ggcgtgctcc agttcttcga gaatgagcgt acctgcgcct
    20581 tcaccaatca ccagcccgtc gcggttttca tcgaacggtg aaggtgtggt tttcggcgca
    20641 tcattgtgct ggctggtggc gaaaagcgta tcaaataccg ctgcttctga aggacataac
    20701 tcttcggcac caccagcaac catcaccgtt tgataaccgt ggcgaatggc ttcccacgca
    20761 taaccgattg cctggctgcc ggaagtacag gcgctggagg ttgggatcac ccgcccgcgc
    20821 aggccgaaaa agaggccagt attaacggcg gtggtgtgcg gcatcatctg cacataggtg
    20881 gtgccagtga tgttattggt gtgcttttcc gtcagcatgg tggcgaactc gcttaccggg
    20941 ccggtactgc cggtcgatga accgtacgca atgcccgttt cgccgctggt taatatcgga
    21001 tcgccaatca accctgcctg ctctaacgcc agctcgctgg cacgggtcga catttgcgac
    21061 acgcggccca tcgcacggat acgcttgcgg gtgtagtgct ccggtagcgt gaaatcatca
    21121 acaggcgcgc caagcagggt gtgcagccca tcgtagacct gccactccgg cattttgcgc
    21181 accgcatttt catatgccag taagcgtgca gaaacgtcct gccagttttc accaaaggca
    21241 gtcacgccgc ccattcctgt aatcactacg cgacgtgtca taacatccct ccgttgatgg
    21301 aaataacctg gcgggtgacg taacccgcaa tatcagacat taaatagctg gcaagcccgg
    21361 caacttcttc cgcctggccc atgcgtttca tggggatcat cgacattgcc tctttcaggg
    21421 cgctctcttc catttcgatc atcccggtat caataagccc cggcgcgatg cagttgacgg
    21481 tgattttgcg cttcgcgagt tcgatagcca gcgcttttgt tgcgccgata attccggctt
    21541 tggcagcgct gtaattcacc tggccgcgat tgcccattac gccggaaacc gaggatagcg
    21601 tgatgatgcg cccaccctgg cgtgcgccga tcatcggcat aatgcacggc tgaatgacgt
    21661 tgtagaaact gtcgaggttg gtgtggatca ccgcatccca gtcgtcatcg cttaaagccg
    21721 gaaaagcggc atcgcgggcg atcccggcgt tactgaccac gccgtaccag gcaccgtgtt
    21781 gcgcgatttc gtgctccagc acttcccgac actgttcgcg gtttgcgacg tcgaaactga
    21841 gcaaacgccc gttaccccca ttggcgacaa tggcattcag cgtttcctga gcgcctgtgg
    21901 cgtcacggtg atagtgaacg ccaatgttaa agccgtctgc cgccagttgg caggcgatgg
    21961 cgcgaccaat gcctttgctg gcaccagtaa ccagaactga acgactcatc aggacgctcc
    22021 ttgttgaaac agggtggtaa gttcttccgc ggtcggctgg aaggtgttaa tgcggcccgt
    22081 cgcctcgccg tcgttgatgc tgcactcaaa actgccgaag cgatcgtctt gcatcagcag
    22141 tttgacggta atggtcagtg tctggcctgc gggcagaatg ccagtggtgc aaagcaattc
    22201 acgcgcgccc agtatcatcc ctaactcaat actgtttttg ccctgctggt ggcgatgcca
    22261 gccagaccag acgccgacgg tttgcgccat cagttcaagg gtaaaccagc cggggagatt
    22321 gccgtccggg tcgagaaacg gtgccagtac gccactgggc gaaaccgtca cgcggcacac
    22381 cgcgctgtca tcgctgacgc tcaccacgtc ttccagcagt aacatgggcg cgtcgtgggg
    22441 cagataagcg ccgggggata aatagtggct cacgaaaccc tcccgagcag aatgctggcg
    22501 ttattgccgc caaaggcgaa ggaattggac aggatcaccg gacgtgccag cggctgcggt
    22561 ttttcgataa tgccacacgg tggcagcgtg ggatcgcgcg gcgacaggct gaagtcctga
    22621 gcgggcagcg gtaaatcacg ctgtaaaatc aacatactaa ttgccgcctc ggtaatgccc
    22681 gctgtgccca gcgtgtgtcc ggtcaaatgt ttggtggagc tgcacggtac gcgttcgcca
    22741 aacagggcgt taaccaccat tgattcaatc tgatcgttga gttgtgtggc ggtgccgtgc
    22801 aggttgatat agccgacgtc atctggcgta agctgcgcat cggtcagcgc ctgattaata
    22861 gcgcggatcg ctccttcgcc ctgcggatgc ggggctgaaa tatggtaagc gtcgctcgat
    22921 tcaccaacgc ccagcaacgc gatgggctgc ggttcccggg tcagcagcat tagccccgcc
    22981 ccttcaccaa tggtgatacc tgcgcggtca cgaccaaacg gctgacataa ggttggcgac
    23041 agcgattcga ggctgtgaaa accgttaatc ggcatccggc tcagggtgtc tgcgccaccc
    23101 acaatggcgg catcgaccag ccccgcttcg ataagcctgc gcccgctgat tatcgctctg
    23161 gcgctggaag agcaggcggt tgaaagggta taggccgggc cgtcgagcgc cagccagtgg
    23221 ctgagaaaac gcgacggatc gccgagttcc tgttgcgcgt agtgccatgt ggtgctcggc
    23281 tgcccgtggg tgcgcaggtt aacgtgcgtg tcgccttcat gcaggccgga ggtgctggta
    23341 ccaagcacaa tcgcaatgcg ctcgcggcca tatttcgcaa tggcgtcatc aacctgcggc
    23401 tggatctgcg ccagcgccgc cagcaggatc tgattgttgc gcgagcggtg cgcagcgaat
    23461 ttttccggga taagcggaag ctcgccatcc acgccagcca ggaccgcctg cggatgcccc
    23521 tgcaaccagc ctgcgcgcgg gcgcatgccg ggagcaacac cgcgagtcag gttggttgcg
    23581 atttcgtcaa ggttatttcc cagggcgttg atcatgccga cggcggaaat ataaatcata
    23641 tcagtcacct aagtattgaa tggtgatgtg gtatttaaag acatgctgct caatgctgat
    23701 tggcacgcgt ttgcccttgc gattcagata ggtgatttcc gtgaccagtt tgccgctggc
    23761 gttacgcagc tcgcgtttgt caccgttgtc gcgaagtgtc catcctgcgg gaagttgcgg
    23821 ttgccaggcg ctaatcggcc agtggctgag catcacgtca gccagtacct gacttgcggg
    23881 cggtaactgt gggacgacga tggattgctc ggcgcgtagc ccttgcgcat cgtaggtcac
    23941 cagaaacagg cgaatgccga ccgacgacag cccggcaagg gtgattttct gatcgtcggc
    24001 gttaagcatc actaacagcg actgggtttt gccgttgaag ctgccggtca acagttgctg
    24061 cgaattgact gcgggtgaaa tccctggtgc cggcagcgtg atgcgcgtgc caggttgcag
    24121 ccacgcctgc gggcggcctt cctgttctgg ttgcgagtgg ctacagccgg tgaggataag
    24181 cgtagcggtc agggcgaacg ctcgccagaa tgtggatttg atcatttttt tgttctcttt
    24241 ttatcgggca tagccagcgg cgaaagcagg aaggcggtga aaataccgct caccagcaca
    24301 atgccaaaac tgctgatggc ctgggtggcg ctgaatacca gcatacccag cgtcagcaag
    24361 gtggtgagca ttgccagcgc gatcgccagt agcgaagtta acggtgtacc gcgcggatta
    24421 ctgaaaaaca gcgtgtagtt gataccgatg ccaagcacca gcaccagcgc cagcagcgaa
    24481 aagagattca ccgcctgccc gctcatcgcc agcaccgcca gaccacagcc cagcgaaagc
    24541 accgaaggca ccaggctgat aagccctttg cgccagccga gacgggccac tgcgccgcag
    24601 gcaatcactg ccagcgccac cagcaacaag ccggttaaga cgtagcggta aagtgcgaac
    24661 aattcatcaa aggtgctttt gcgatcaacc caggcaatgc cgcaagggta atatgtggcg
    24721 atttcctgca tcaacgcgct acttttaacc ccttcaaccg gcaccagtac gccgctttcg
    24781 ccgttttcca gcgtcagcca cagcagacgc cagccttcac tggcggggct tgccagccat
    24841 tcatcaacgt tcactggcat ggcgttgaga tccgggttca ctgccgtcag cccggcattt
    24901 tgcagcgctt ttgttactgt cggggccgcc gttttcagca gttgtaaatc ttcctcctgc
    24961 cgcgcaaggg agttcagcgg aatggtgcgg tagttgctga taagcccctc ttttttcgca
    25021 tactcaagtg aggcggtata tttctccagt cgccgcaatg tttgctgtgg cgaatcgccg
    25081 taaaccacaa accatttttg atcgacgctc tgcccggtca gggcggtaat ggctttttcc
    25141 tgcgccagaa tatgctgcgg tagcgcctgt aactgcgaga tatcgtcatc gacgcgtagc
    25201 attgacatcc ccgccagcga aaacagcgcc agcgcgacgg gcagacccag cgacagtttt
    25261 ttattacgcc gccacgctgc cagccagcgt agcatcagcg ccatcgccgg aaccggacgc
    25321 accggcaggc cacggcacag ccacggatgc cagaacagga cggtcagaca ggaggcgctc
    25381 aacccgacgg cggcaaaaat cgccatctgg cgaatgccgg ggaagggggc gagcatcata
    25441 atcagatacg ccgccacggt ggtgagcagc gccagtagca aggcattgcg tactttcgcc
    25501 aggctttgcc acggcgaaac gtcgttgccg tgaaccatcc gctcggtgag ataatagagc
    25561 gtgtagtcag cggaaatgcc gataacgctc atgctcatca ccagcgtcat caggtgtaat
    25621 tcaccgaaaa tcaataaagt ggcgaccgtt cccgccagcg cgccgatgcc gatggaaatc
    25681 acgcaaagca gcaacgggcg taaagagcgg aacaccgcca caatcagcaa tatcaccccc
    25741 agcagcgtag ccacgcccag ggtggagata tcctgcttcg cctgttggct ggcgtaatcg
    25801 ctgtaaaaca ccgtgccgcg cgagagcaac tgtgcctgcg ggtaacgcgt tttcagatcc
    25861 ttttccagcg tattcagggt cgtgatcagc tggtgggttt gctgcatatc aaacgacgat
    25921 cccgccagtt cgccgtgcag cagataccag tagttgccct ggggatcctg cgtcaccagc
    25981 caaccgtcca tcagccgcaa acgctggccg tttttcgcca ttgccagctg cgagccgcgc
    26041 attaacatca gcggatcgtt ttgcagctcc ttgccgctta cgccggagaa tgcggaataa
    26101 agctgggaga ggatccactg tgcctgcgct tcgccgccgt tttgcaggcg ggcgcgggtg
    26161 ttggggtcaa tcaggccgtt gcgatgctgc cagaaaaacg ctccccacgc ctgctggctg
    26221 gcggcatcca ttggcccttt aacgtcgccg agcgcagcgg atttttgcag cagcgtcagc
    26281 cactcctgag cgaccaaagg attagcctct ttaccggggc tgaccagcca caccagttgg
    26341 cggtcaagac gctgcataaa gccgtcattc agcgccggag gaatatcgcc catcgtctgt
    26401 ttgggtaaca tagccagcac gctactgttc agccgtgatt gcggcagcag gatcagcaac
    26461 gccaccgcca tgaccaggca gactagcccc cataacagcg cggggcgttt actgggcggc
    26521 aaaacgttgg cgttcgtcat cggtcagttg cgctggcgtc agttgatgtt gggtaagagc
    26581 aatatcggtg cgatcgccct gtttatcatt aagttgaatg ctctccagat aggttttccc
    26641 ggcgagatcg atggtgttga aaattttatc cagcggcgtg gtggtcggcg tcaggcgcag
    26701 cgtccagcgg ccttcgcctt tgtcagcaaa ttcgacgcgg aagttttgtt ccagcacttt
    26761 gcgatcggcc tggaacaacg cgcgcagcag gtggttaaac tggaacatct gcgggttgtt
    26821 ttctgccgtg atgatttgcg gcggctgacc gttgatcacc tgcaccatgc gtttatcgtc
    26881 cagtaatagt tgcatgggga acggtgaggt ttgatcccac aacagcccct ggtcgcgggc
    26941 gatcaacatc tggccctgag atcgcagcgg ctgcggcaga tctttaatcg tccgggtttg
    27001 atcgaaatgg gcgcggatca ccggttgttc ggtaaagcgt tgctgaagat cgtccagggt
    27061 cagggcgctc acaaacgggc taatcagcag cgccagcagc ggtaaaaatt tcatggtttg
    27121 actcccatgc gttcaaacag aatatcgggg ctgacaaagc acagttcgcg actttgctcg
    27181 tcgaccgcga cctgaatggt gtatccggtg gtggcgcgtt tgcccgtttc ggcatcgaaa
    27241 atctgatagc caatccgcag acggttttcg aactcttcga tatgcgcacg cacgcggatg
    27301 cgttgctcaa aggtgagggc gtggcgatat ttcacccgcg catcgaccac tggccacaaa
    27361 taaccggact ctttcatttg ccgatagccg taattgaact ggttgagcag cgcctcgcga
    27421 gccacttcaa agtagcgaaa atagttaccg tgccaggcca cgcccatcat atcgacgtcg
    27481 tgaaagggaa tggtcagctc gacttctgcg gtaaatcggg gatcgttcag caccctttac
    27541 tccttgtcct gaatttccgg cagttgccag aaatcgaaaa aattaaacca gtcgagaggc
    27601 gactggagcg cgtaatgttc cagacgcgcg gcgtaatgat cgatagcgtt ttgcagcgcc
    27661 tgttggcgtt cgccgcgcgg cagctgcagt gggtcggcaa agctttcgca gtgaatatgc
    27721 agcttgccgt gctggcgcag ggcgaaaatc agattcaccg ggcagcgcaa aatagaggcg
    27781 agaataaacg gcccctgtgg gaaaggcgca ggctggccca taaacgaact ccagcagacg
    27841 cgccagtcgc cgccgcgttg cgggttgacg gcgatgcggt caccgacaat cgccacccat
    27901 tcgccgttat ccagcttctc tttcagcagg atggcggttt ctgggccgat atctgttacc
    27961 ggcatcaggt taatgcctgc ctgaggagcc atctcctgca ttatctgttt aaagcgttgg
    28021 gcgttttcgc taaacaccag cgcgttaatg gttttgtagc cctgaatttt tgccagcgcc
    28081 cggcacactt ccacatcgcc aagatgcgag gccagcagca atttgccgcg cggatcgcgg
    28141 acgtcaagcg ctgcttctgc gcctggcgca aacagcacat cacgccctgg ttgtagctcg
    28201 ccgcgccagc tggcgatttt gtcgagcatg gcattaccga aacgtagaaa gtgctgatag
    28261 ctgttgagtt ttgccgcctg tggttgatgc tgtcgtacac gggcgagcca gtcttgcgac
    28321 gctttgcgcg cacgagaagc agtgagccag tagacgccca ccaccgggta aagcagcgcg
    28381 ctaaacgccg ttcttcccag cagacgccag accagcagca tcaggcgcat tccccataat
    28441 cctttcactt cactctgccg cgcccagtgg caggaagagc ggcgcatcag cagtgaagga
    28501 atacgcggca acatgccgaa aaacagacgc gtgtgcatga gcgagatgcg gacgttatct
    28561 ttcagggcat caaaatgcga aagcccgtcc agtggatagg tgacgcgggt cggcacgaaa
    28621 tagctggtat ttccctgcca gtagaggcgc accatcactt cggtgtcgaa atccatccgc
    28681 ttgccgatgg tggcgtgttt tgccagttgc agcgttggcg caaccggata aacgcgaaaa
    28741 ccgcacatgc tgtctttcag ttgcagggag agcgtttcga tccataccca gacgtgggtg
    28801 acccagcgcc cgtaaagccg tgagcggggg atggagtcat cgtaaattgg ctggccggag
    28861 atcagtgccg caggttgttg ttcagcgaga gccagcagtt tagggatatc ttcaatcgcg
    28921 tgctgaccgt cagcatccac ctgcaccgca tggctgaacc ctgcgtctgc cgctgcctgt
    28981 aagccacgca tcaccgccgc gcctttgcct gcgttttctg ccaggcgaat taaggtcacg
    29041 ccaggctgtt cggcaagcag actgtccagt tgctgttgtg tggtggcatc gctgccgtca
    29101 tccaccacaa tacagggcag attaaatggc ttaagacgcg ccagcacgcc cggcatcatc
    29161 gcgccgtggt tgtagcaggg tatcaacacg cagggagaaa agtttaccga cataggcgga
    29221 tcttcccgct actggcggtg tggcgggcgt caccgtcgtg acgctgatag ctaaagctca
    29281 gaatctggcg ttcttcctgc cagttaagcg cgagcgtgac gcggttttcc ggcagcaacg
    29341 gagactggaa ttttatgttc tgaatggagt gaaaacgcca gccaggcgcg agcagattga
    29401 gcgcgtagct catcgcccag tcgatttgcg caactcctgg gagtaatggt tgcacggcaa
    29461 aatgaccgcg aaaccagaac agatccgccc tgagatgcag aacaatttca aggtgattcg
    29521 tttgtgcctg atggcgctcg atttcatggg gcttcatgaa ataactcctg taattgcgca
    29581 tagacacgct tgttcatact gtttactggc atttcatcaa taacgcgcca gtaacgcgga
    29641 atagcaactg gctccagcgt cgggcgcagt aaacgccgcc aggtcatttc ctggctatgc
    29701 ccgccgccgt tctgccattg cagatgagct tcgtcatcca gcaccagcaa aacgcctatg
    29761 ctctgacgcc cgccgcgcgt aacgggaact gccgccgcct cgcgaatgcc gtccagcgcc
    29821 agtagacgtt gttctacttc ctggagtgaa atacgttttt cttcaatctt aacgatgcgc
    29881 ccacgacgcc ccatcagatg gaactgacca tcttcgctaa actgtaagat atcgtcgagt
    29941 aacatgccgt tatcttccgc tatcagcggt gaaaagaggc gaaaggcatc gccttcggcc
    30001 tggaactgca tacctgggag gggtagccag cggcgttgct cttgttcgcg gtagcgccag
    30061 gcgatgacgc cagtttccgt gctgccgtag atttcatcag gccagacgcg cagccagctc
    30121 gcggtatgtt gtacatcctg ccacggcagc tcaccgccag cagagagaag cacctgaacg
    30181 ggtggcggag aaagctgggt atccaggcgc ttcaaaaatg ccgggctgct gatgaagata
    30241 tagcgatgct gatgactcaa cgcggcaaac tgttcgacat accagagcat ggcggcgtgc
    30301 agcggtaatc ccaacgccat gggcaggaaa acgcgaaaag tcaggccgta caaatgctgc
    30361 ggcaatacgg aaccgacgac acgacaaccc gccagacgtg cgcctaatcg ttcagccagc
    30421 agttctgctt cacgatccag caaatgaaca ggtttaatcg cccgtttcgg ctgtcccgtg
    30481 gaaccggagg taaacagctc gataaaagcc tctggcgcta tagcggcaaa ggtgaaggat
    30541 tgtgtcgcga tttgtggaga acttgctacc aacagcaatg aaccttgcca gttaagttcg
    30601 ctgtcgctaa gcacaccatc aaaaagttca cgttgctcgt taagctggat aacacggtta
    30661 tgccccggaa gcaccggcgt ttttccggcg tgtagcgtgg cgagcagggc aacgataaac
    30721 agatagctgt tctcaaagca cagcgcccag cgttcacccg gctgttgctg caagcggcag
    30781 ataagttgcg cgacatcgtg acgcaaatcg cccagcgtcc aggtactttc gttcagccac
    30841 gcaatgggtg tgtcatcagg gcgtggcgca gtcagccagc gtgcaagcgg taaggtctgc
    30901 ttcatgcgcg atctcttttc atcacctgac ggcgcagcag ccattcacca gccattaacg
    30961 tccccatcaa caaataagca atcatgccgt tccaggcggt ccataacgac atatcggcat
    31021 aaagcgcggt aaagagggca attccaccgt taatgatgaa aaagccgcac cagatttgcg
    31081 tgacgtggcg ggtatagcgc acgccctttt ccgggaggtc tggctcctgc aaccgcgcca
    31141 gccgctcaac tatcggcatt gccgaccaca gcgaaccgcc aaaaaccgcc agcatgaccg
    31201 cgttaaccac taccgggtaa aacagcagta gctggtgcgt tttcaacata aagctggcga
    31261 cacataatgc gatgcccacg acagcgacga gttgcgtgac ggcttgtaaa ggccccgcct
    31321 ggcgacgggt ctggcgcaga cgcaacaaaa gcagcaacgc cattagcggt aacagccagt
    31381 gcaggccgtt atgcgctaag ccaaaccaaa tcacaaacgg ccaggcgagc agcaataatc
    31441 cagtcagcag cttaatcatc ggtagcgagc gaatgcccga catcagatca ggcttcttgt
    31501 agcaggcgtt ctacagcctc gacgacatcc tggactgtac gcactgcttt aaactcttcc
    31561 ggcttgattt tcttaccggt tttcttttgc aggtgcacaa tcatgtcaac ggcgtcgatg
    31621 ctgtccagtt ccagatcttc gtacaggcgc gtttcaggtt tgatgtcctg cggatcgatt
    31681 tcaaacagct taaccagcag ggctgagact tcctgataga cggtttgttg ttctgtcatg
    31741 atggctttcc tcaggcacgt tgcgcagcaa tgaaagaggc cagagtggcg acggagaaaa
    31801 agtgctgacg catctcttcg ctttccgcag agagcacgat gccgtactca ttttttactg
    31861 ccagtcccag ttccagggca tcgatagagt ccagccctaa accgtcgcca aacagcggcg
    31921 cgtcggtatc gatatcatca ggcgtcaact cgtccagatt gagggtgctg atgatgaggt
    31981 ttttaatttc cagataaagc gcttgcatta tttattcctg agagaggtaa acaaccttgt
    32041 tgtaattgaa gcaaaagatg ccggtttaac tgccttgctg ccagcgccgg ttcttgtgaa
    32101 gttgcatcgt aaaatttatt gatttcaacc cgaccatgga ctgcaaccgt aaaaaacggc
    32161 cggtcaggtg gaacgtcata ccatttactt tgtttatcca gcagatgctc gctacagccg
    32221 attgtcacga tgcgtaaatc gctggcgcag cgcacggcaa tattcgccgc cccgcgctgc
    32281 agcgtcattt tttcaccggg cttcgttcgt gtgccttcgg ggaaaatcag gatagtgtca
    32341 ccctgtgcca gtcgctgttg gcagcggggt aacagagttt cagcttcgct gttgattaaa
    32401 taatctgtcg cccgcacgac gccgccaaga aaaggatttt ttaacagcgc gcttttcacc
    32461 aggcagtcag tttctggcat gactgacgcc agcagcacgt aatcgatcag cgtgggatga
    32521 ttggctacga cgaggcaacc gcgttcctga tgtaagattt cagcgccatc aatacgataa
    32581 tccagtacgc caagcccttt cgcgacagtt aaaaacaggc gaaaactggc ggcaatgctg
    32641 cgacgcgcca aacgacggcg gcgggaagta tcccacacca aaactaacaa tatgttaaac
    32701 cacacgacag aaagcagcag gccgccgaga ccaaacaggg caaaacataa cccggtcatg
    32761 accagtcgcc aggtccactc cagacgtgac atcagcttac tcatctgtgg ctccagcgcc
    32821 attgcacgcg ctcgccaggc agtgaaaatg tgtctacatt ttgtaaatag tgctgtaaaa
    32881 acaacatgct ttgcggcagt gttgtttcat tgcccgcaat ggctgactgc gtttcacatt
    32941 gccagtcatc gccagcttca atcaccagcg cgacggcgta cggccaggtc ggcatttctg
    33001 atggcagttg tgggtgataa aactcgggca ggaaaccgtc aaaatcgacc atcagcacgc
    33061 gctggtatcc cgcttgtagc aggctaagca cttcacacaa accttgctgg aaactatcgc
    33121 gaccagccga aagcgatgac gaaacgctcg gctgtttggc cgcaatggtc aggttaccca
    33181 cggaagagtt gtgtacggag agcgcgaagt ccgtcggaga aagcgcctgt tcggttgcca
    33241 gtgcatgaac aatgcggtaa ttgcgctcca gttcgccatg acgactggta tacaaaacgg
    33301 cgtctggttg atggcggcgt agcattgcca gaccgcattc aacagccagt ttgctcccgg
    33361 agctgaggcg gcgggcggtc atcattggca gttcacttaa ttttgccaac ggtgctgtcg
    33421 ggtcaattgc ctgaggctga cgtgaccact cctgccattg cagcgtttca ctaagtccag
    33481 gcgctcttgc ctgccagtcg agaatgttta atgcaaattt catcaacgcg cgcccgcggt
    33541 aaaccaaaat tattaagtga cgattatttc ttttggcata tcaaaagtgt atgcccaacg
    33601 cctaaattat cgtggcgctc tgctacctgg aatcctgctt tattcagata gtcgtaaaat
    33661 ttctctgcac tatagaaacg gctgttaccg tttgccatgc aggtaaaata aagtgaggac
    33721 gcgttcaggc taaacgatgc tgcttcaaac cgctgcgcat cccagaacaa ctccatgatg
    33781 caaacttttg cgccaggctt cattacgctg gcaattttgc tgagtatgct aattatttgt
    33841 tcgggtgaaa aacagtctaa aaattggctc atccaccaga tatcagcctc gcccggtaat
    33901 ggggcatcgc ttagcatatc caccgcatgg aaatctatgc gatcagaaaa acctgcattc
    33961 gcgatatttt ctttcgctag tacgatttgt tgtggcagat ctaataacgt aacggcgatg
    34021 ttttcattgt atttgcagca acgcaaagcc cacttacccg tattgccgcc tacatcgtac
    34081 aatgttgtgg ggttgttggc gaatacataa ggcagtgcgg cgttaaatgc gccatctgaa
    34141 tagtaatgat caaacgcaaa ccagctttca cgagcggcgt ctggtaattg cgaaagtgcc
    34201 ggatatatcg tcgaccagtc gccaaatact tttaacccgg aaggttttcc ttcattgagt
    34261 gaatcggcga ggaagaagag cccctggtaa cagacatcct gggtgaaatc catattcacg
    34321 cgagtcatag tgtcatgcag gaggtaatga ccgattttcg ccaggtaata aattccctct
    34381 ttacaggtaa taattcgccc gcttaagccc atatctaaca gaacgctggt agcatattcg
    34441 ttgatatggc tatgttcggt gatatcagcc agcgttgcgc catgtttgcc ttgccgatcc
    34501 agatatgaaa gtacacccgc attacgtagg cagagtgctg tttgaaaaag cataggggca
    34561 aaggcaatgc gttgagcttc ggtaatcgca tccagtgcgc taagagaatc ctgttcgtac
    34621 atgttgggca caacctgtta agagggaatt aaattattat cggaccgcaa cggcccgact
    34681 ttaggaagaa ttataaatct gcgcccgctg acaaattcag ttgaatatct ttgtcgagat
    34741 tgcggaccca gcgattgtag tttttatgaa tttgtccgcc tgacgcttgt aggttctggc
    34801 tattttcata attgatatcg tagctggatg ccgtgtagtt aatgcgtatt tccgctgaat
    34861 gatcgcgtga ctgttgacgt gctttaatta tgccttgtcc ggcttcggac ataatccatt
    34921 tgcgttcaac gccagctttc agaatcgctt ttttcacttg ttcctgggta tgaccagcgc
    34981 tgacggttga gtgaacctga tcaattggcg cagtacgcgc gcaacctgct aaagcaccca
    35041 caacagcaat agccgcaaac catttcatgt aatgtctcat tagaacccca ttaaagtatt
    35101 gtttttacga gtaaagcaag ttttaactgt aattgctggc atcactattc tttatttaga
    35161 gaaaaaatta cttgtgagat aagtcagtaa gtgcacatca gagtaaaaat ctctgaaatg
    35221 actaaattac tgccagcagg gaccaactat aagtgagtga gaaaaatgag gcaataaaaa
    35281 taacacaatg ttatcagtgc attctttgag aaggatttaa tcgctattcg gcgtttatat
    35341 atatttgact taaggaatgg tttttttaaa ttaattttct gaataatgtt tcatcatttc
    35401 ctcaaatcaa atggccactg ttagataaac ggtggccatt gcctgctcag gaggtgctat
    35461 taacccggta accgactttt cggtctgccc ggccccagta aaatcgccag tttgctgccg
    35521 cctttcgtcg tttccatcca gattttacac acgctggtta atggcaccga aagtagcatc
    35581 cctaccgggc cgagcagcca tccccaaatt aacaatgaaa gaaataccac catggtggac
    35641 atccccaggc gatggcccat catccgtggt tctaaaatat tgccgatgac catatggacc
    35701 actaaaaaca atgcgccgac cagaatacat tcgtaaacac cattaaacag cagcacctga
    35761 atcattggcg gtacggcgga aattaccgcg ccgatattgg gcacgtagtt aagcaaaaac
    35821 gccagtactg cccacatcag cgcaaactgt acccccatca gctccagccc cagccagacg
    35881 atgacacccg tccataaact gagtagcgtc tttaacgcca gataatgcga aacgccttta
    35941 agtgcgcggt gtaatcccgc gatgtgaatc tgtgggttat tcagcgcaaa acgcattttg
    36001 taagggacgt ggcgcacttc aaacagcata aaaactacgg tcatcaccag caaaagcacg
    36061 ctcgccattg ccccggaaag cccggtcatt agcgctgtgg tgaaggtaac cactttttcc
    36121 gagtccatcc gctgcagcat tcgctccggc gacatatgca aattaagaaa aggcaacatc
    36181 tcctgcaatt taaaaagttt gcgcgtcagc tccttattaa acttcggcag catagagata
    36241 aattcgttaa acgatgccgc cagtacgccg accagcgcgg ttagtgcgat cagcatcacc
    36301 accactacaa tcgtaatggc aacggggcgt tgtactcccc gacgaataaa ccaggtgacg
    36361 agcgggttga ggacgatggc aaaaaacagc gccagtaaca gctgcacaat gatatctgcc
    36421 gctgcgtgaa tgcccgcgag gatcactacc agcgaggcca gcttgagcag aatgtgcatg
    36481 cccgttttat cgggttgagg ggtttccatt ggggcttcct tgtgactttt tgtattaagt
    36541 gtagcgggag tcacgacggc aatattctga ttcttactgc tgattttcac gtcagcacgt
    36601 ggtaaaaatg aaacactgtt gtaaaaatgt ggtgatcctc atgcccgaac ccgtagccga
    36661 acccgcgcta aacggattgc gcctgaattt gcgcattctc tccattgtca tgtttaactt
    36721 cgccagctac ctcaccatcg ggttgccgct cgctgtatta ccgggctatg tccatgatgt
    36781 gatgggcttt agcgctttct gggcaggatt ggttatcagc ctgcaatatt tcgccacctt
    36841 gctgagccgt cctcatgccg gacgttacgc cgatttgctg ggccccaaaa agattgtcgt
    36901 cttcggttta tgcggctgct ttttgagcgg tctgggatat ctgacggcag gattaaccgc
    36961 cagtctgccc gtcatcagcc tgttattact ttgcctggga cgcgtcatcc ttgggattgg
    37021 gcaaagtttt gccggaacgg gatcgaccct gtggggtgtt ggcgtggttg gctcgctgca
    37081 tatcgggcgg gtgatttcgt ggaacggcat tgtcacttac ggggcgatgg cgatgggtgc
    37141 gccgttaggc gtcgtgtttt atcactgggg cggcttgcag gcgttagcgt taattattat
    37201 gggcgtggcg ctggtggcca ttttgttggc gatcccgcgt ccgacggtaa aagccagtaa
    37261 aggcaaaccg ctgccgtttc gcgcggtgct tgggcgcgtc tggctgtacg gtatggcgct
    37321 ggcactggct tccgccggat ttggcgtcat cgccaccttt atcacgctgt tttatgacgt
    37381 taaaggttgg gacggtgcgg ctttcgcgct gacgctgttt agctgtgcgt ttgtcggtac
    37441 gcgtttgtta ttccctaacg gcattaaccg tatcggcggc ttaaacgtag cgatgatttg
    37501 ctttagcgtt gagataatcg gcctgctact ggttggcgtg gcgactatgc cgtggatggc
    37561 gaaaatcggc gtcttactgg cgggggccgg gttttcgctg gtgttcccgg cattgggtgt
    37621 agtggcggta aaagcggttc cgcagcaaaa tcagggggcg gcgctggcaa cctacaccgt
    37681 atttatggat ttatcgcttg gcgtgaccgg accactggct gggctggtga tgagttgggc
    37741 gggcgtaccg gtgatttatc tggcggcggc gggactggtc gcaatcgcgt tattactgac
    37801 gtggcgatta aaaaaacggc ctccggaaca cgtccctgag gccgcctcat catcttaaaa
    37861 cttactgaat aaccagcgta ttaatgatgt tttctgcggt ggtctgcgct ttttgctgat
    37921 cgtcagctgg cagagtaatt tgcatggtca gcagttgatt acccacgtta cccagaataa
    37981 cggaagagta cgccgtctgg cctttcgcgg agataatact gtctaactgc tgcattttgt
    38041 gacctttcag ctcaatggct ttattggtga ccacttgcag ctgtggatcg cggctacgtt
    38101 gctgatcttc cagacgcttc gccagcaccg ccagatcttc tttcggatca tcgcccatga
    38161 tgacgatgac tgctttctgc ccggtggcgt cggaccagac atgcatgtta ttggcctgcg
    38221 ttcccagctt accgctctgg tcggtcatat ccgctggcag cgagaaactt aacttgccat
    38281 caagcaggtt gacgggattc ccggtagcgt tactttccgc gaccgaaccc tgcgccgtag
    38341 cgttagtgtc tttatcatca caggccgcaa gccccataac cagcaggcca attccgacat
    38401 atttaaccag attgcgcatt gacttcttcc tttcgataaa cggccataac ggctcattca
    38461 tccatcttat cacaactctg ataacgaacc tttaactcgc ctgcaaagcg ttgatttcgg
    38521 atttatctgc cagtcttttc aacagcatat tgagtaatac gccatacatt ggcaggaaga
    38581 aaacgatact gattaacact ttgaaacagt aatcgaccag cgcgatttcc atccagtgtt
    38641 cagccataaa ggcatccggg ctacgccaga aggcaatgaa gaaaaaggcc agcgtgtcgc
    38701 tgacgttacc gaacagtgtg gacgctgtcg gtgccagcca ccagcggcga ctctgacgca
    38761 ggcggttaaa aacgtgcacg tcgaggatct gtcccagcgc gtaggccatg aaactggcgg
    38821 tggcgatacg ggcgacaaac aggttgaagt gggcgagtgc gccgaatccc tgccatgaac
    38881 ccatatagaa tagcgacgag atgacgtagg agatcagcaa tgccgggatc atcaccgcaa
    38941 agatgatacg acgggccagc ggtgcgccaa aaatacgtac ggtcaggtcg gtagcgagaa
    39001 agataaacgg aaagctaaac gcgccccagg tggtatggaa accaaaaatg gagacgggaa
    39061 gctgaaccag atagttactg gaggtgatca ccagcagatg aaataacgat aaccagaaca
    39121 acgccttata gcgttgagtt tgcgagaaaa cgttcatatt gtaccttttt gattaaccat
    39181 tggggtgagg gaacccaata cgtacgacac gtctgttacc ttaacataat aacgtgctta
    39241 cccttttttc gagccgccgc atgatactgc tttacgttga caatgcaatg gttaattttc
    39301 acgcaatcgt taacctggtt tgcttacgga ctcacagggc gtaaactagc gccgtttttt
    39361 tatgtgatga gaagaaaatg accgatctct tttccagccc tgaccacaca ctcgacgcac
    39421 ttggcctgcg ctgcccggaa ccagtcatga tggtgcgcaa aacagtgcgc aatatgcagc
    39481 caggtgaaac attgctgatt attgctgacg atccggcgac gacccgcgat atccctgggt
    39541 tttgtacctt tatggaacac gaactggttg ccaaagagac ggatggactg ccttatcgtt
    39601 atttgattcg taaaggcggt tgacgggggc agattggctt cgatgccgcc ttttcccctc
    39661 accctaaccc tctccccaga ggagcgaggg gaccgatcgc gctcaatgtt gcgatcggtt
    39721 tgccttatct cctgcgcaac aatcttaacg cattcgctgt caccagcacc gtcgcacccg
    39781 tatctgccag cactgccagc catagtccgg tcatccctaa cagcgtggtg acgaggaaga
    39841 tccctttcag ccccagcgca atagtgatgt tctggcggat attggcgtga gtggcgcgtg
    39901 ccagttcaat catttgcacc agaccgcgca ggtggttatg ggttaatgct gcgtcggcgg
    39961 tttccagcgc cacgtctgtg ccgctgccca ttgcaatccc gatggcggca gctttcatcg
    40021 ctggcgcgtc gttaataccg tcaccgacca tcgccagcgg cgcatgttga ttcagctcgg
    40081 tcaccgcttt gactttatct tccggcaaca ggcccgcttt aaactccagc cccagctccc
    40141 cggcaattgc cgccgctgcg cgtggattat cgccggtgag gatcacccct ttgacgccca
    40201 gcgcgttcag ttcactgatg gcagttgcag catcggcgcg cagggtatcc tgcaatgcaa
    40261 tgatacccag cacgtcatca ttacgtacta ccagcacaac cgtttgcccg gcgctttcca
    40321 gttcattaat caaaccagca aatgcatcag cgggatgttt cccggcagcg catatcaaca
    40381 cgcgctcacc gttaacctgc gcttcaatgc cagacccgac cagcgcccgc tgtgattcgg
    40441 cggcgggaat ggcgagtgca gcaacctgtg cttcgcgcac gatggcctgc gccagtggat
    40501 gcgtcgcgcc ttgctcgacc gccgccgcca gtgtcagcag ttcagattca ctaatacccg
    40561 ttgccggatg aatcgcggta acgcgcggtt taccgacggt cagcgtaccg gttttatcaa
    40621 acgccacctg ggtaacacga cccagctgtt ccagcgccgc tccgccttta atcaacgccc
    40681 cacgacgcgc tgccgccgcc agcccggagg taatcgccgc aggcgttgag ataactaacg
    40741 cacacgggca gccaatcagc agcagcgtca gccctttata aatccactcc tgccagctgg
    40801 tggcaaacag cagcggtggc accagcgtca ccagcagagc gacggccata atcgcgggcg
    40861 tgtaaatacg gctgaaacgg tcgataaacc gctcaatggg tgcgcgacgc tcttcggctt
    40921 cttcgatcag tttcagaatc cggtcaatgg cgctggcacc cggttctgac agcacttcca
    40981 gcgttaccag acggtctacg ctggtggctc ctgcaggaac tttatcgccc gttgcgcgct
    41041 ccaccggaat agattcgccg gtcagggcgc tttcatcaaa actggcaaac ggtgagagca
    41101 gtttaccgtc ggcaggcaaa cgtccacctg cggcgacttc aatcacatcg ccagggcgca
    41161 ggctgttaat cgccacctct tcccgctcac cgttacgcag gcgcgtggcg gtttctggtt
    41221 tcagcgccat taacgcgctg accccttgac gcgcgcggct ggcggcccag ccttccagtc
    41281 gttcaccaat caaaaacagc agcaacacca tcgcagcttc agccgttgcg ccaataaaca
    41341 gtgcaccaat agcggctacg ctcattaagg tttcaatggc gaagtagctg ccggatttga
    41401 tcagccgtaa tgcctgacga gcaatcgggt acagcccaac cagcgtggtc gcgataaacg
    41461 ccagttgccc gaacggatga ttaaattgct ccaaacccca gctgattgcc atcatcacga
    41521 taagcgtaat cagcggcaga ttctctttca ggcgcgatgc ttgtggctcg tcggcggcct
    41581 gctcatcgcg cagggaatag cccgcttttt gcaccgcaga ttcaacttgt gcacggatgt
    41641 cattgtcggc atcaaccacc agtttttcgg tggcgaacaa cacctgtacc tgattcacgc
    41701 ctgcaagctg gcgcacggca ttttctacct tgcgcgcaca ggcggcgcag tccatgccgc
    41761 tgactttcca gctatagcgg gtgccggaga cgttttcaga gagagttggc gtgctggaac
    41821 atgcgccgtc gcagcaacag tcgttggcgt tctgtaccgt ggttagcggt ttgaacgcag
    41881 caaattgagg ggctttcttg ccgtgattgt caggagtcga catggcatcc tccggttaag
    41941 ttttttctca ttaaccgaag gatacactct ggagtcgact ccagagtcaa gttttatcag
    42001 agatacagcg agcggacgat gaggaagtga ccggcaaagt agcaggccgc agcaatcgcg
    42061 ttgtccgcac ggaagcgtcg gcgatagtga ctacccagcc agacaaagtt actgataaac
    42121 agcaacgaag cgccgacaaa cgcagagagt gccggagcgg tcggacggaa gaaccacagt
    42181 tcacctgcca gccacaccat caccagcgtc atgccgataa acgtgcagat aggccaacga
    42241 tactcttcca gccgcgtcca gataatcgcc agtaacagcg cacccagaac cagcaacacc
    42301 agtggcagag gccagaagaa agagagcgtc atctgactgg cgaaatagat ggtgtacagc
    42361 aggtgcgaga ggaaaaacgc gccgatggcg tacatcagac gttgacgtgg caacagggtt
    42421 agcgcatcgc ccagcagtga ggcgcacagc cctgccagca ccagatagct aatggcgtcg
    42481 aacatcggcg cttgccaggc cagtaacagc aggagaagaa gggttaacgg tttaaacacc
    42541 cagcgttgcc aggttggccc acgatacgat gcatccacag atagccatgc ggaaagacag
    42601 acagcgataa acgaccaaag catcttagtt ccttgaattc gttatttttt cgtaagcgga
    42661 atgcctacgg tcttgcttcc agtttagtgg cgagggtgga cggatgacaa caccataact
    42721 ggcagggtat gcttatttcc gcattttccg atgagggata aagatgagca aaccacctct
    42781 tttctttatt gttatcattg gcttaatcat cgtcgccgca tcgtttcgtt ttatgcaaca
    42841 gcgacgggaa aaagctgata atgatatggc accactccag caaaagctgg tgttggtgag
    42901 caacaagcgg gaaaaaccga ttaacgatcg ccgttcgcgc cagcaggaag tgactccggc
    42961 aggcaccagt atgcgctatg aggcaagctt caaaccgcaa agcggaggaa tggagcagac
    43021 gtttcgcctc gacgcccagc agtaccacgc cctgacagtg ggcgataaag gtacgctgag
    43081 ctataaagga acgcgctttg ttagctttgt tggcgaacaa taaggttatt ttttaacttt
    43141 aaatttcttc tgccagacca gcagttcaaa cacgccgaaa aggaaaatcc gcaccttttc
    43201 ggcggtggtc atctgcgggc catctttcgg taaagtggat ttcaatagcg ccagttgcat
    43261 accatgcatc agcacggtaa aaatcagcgc cacgttaacg aaaatattca gcgggcgcgg
    43321 gaagggatgc accaggttga ggattaaaaa tccccaaaca cagagcatta acaaacgacc
    43381 aatattaatc agcatcactt tctccttgtg cttcgcgttg atacagccga taagccacct
    43441 gacccgccac tttttcccga tgtaacgacc agtttgccgg aacggtgggc agaccgtttt
    43501 cgacttcgct ttcgacataa atcagggttt cgtcagccag ccagccgtta tcttccagta
    43561 aatttatcgt ctcttctaac aaaccacggc ggaacggtgg atcgacaaac acgatattat
    43621 gcggtgtgcc tttttgcgcc aggaatgaca ttgcgttgct gttcaccacg cgtgcattgc
    43681 ctgcttttag tgtcgccaga ttcttaatta actgctgaga aactgcacga tccatctcaa
    43741 ttaacgtggc ccccgcagcg tagcgcgata acgcttccag ccccagcgcg ccgctcccgg
    43801 cgaagcaatc cagacattgg gcgtcaacaa tgaccggagc cagccagtta aacaacgttt
    43861 cgcgtacgcg gtcggtggtg gggcgcagac ctgggctatc aggaaccggg agtttacggc
    43921 ctcgccactg cccgccaata atgcggattt ggccgctgcc ggaatgattc ggttttttca
    43981 tgataaattg ctcaatccgc cagataacac atacttgcag gcggtgatgt gaattaagtt
    44041 aagtgataga ctatttcatc atttttttag ctgctatgta catagcgtta acgctgtgcc
    44101 atgaagcaac agcgaggagt gtagtcgcaa atggcgaaag aaaaaaaacg tggctttttt
    44161 tcctggctgg gctttggtca aaaagagcag accccggaaa aagagacaga agttcagaat
    44221 gaacaaacgg ttgtagaaga aatcgttcag gcgcaagagc ctgtgaaggc ttctgaacaa
    44281 gccgtcgaag agcagccgca ggcgcatact gaggccgagg cggaaacttt tgctgccgac
    44341 gttgtggaag tcactgaaca ggttgctgaa aatgaaaaag cgcagcctga agcggaagtc
    44401 gttgcacaac cggaaccggt cgtagaagaa acgccggagc cagtggctat cgaacgtgaa
    44461 gagctgccgt tgccggaaga cgtcaacgcc gaagaggttt cgccagaaga gtggcaggct
    44521 gaagcggaaa ccgtagagat tgtcgaagcg gcggaagaag aagcggctaa agaagaaatc
    44581 accgacgaag agctggaagc acaggcgctg gctgccgaag cagcagaaga ggcggtgatg
    44641 gtggttcctc cggcagaaga agagcagccg gtggaagaaa tcgctcagga gcaggaaaaa
    44701 ccgaccaaag aaggtttttt cgcgcgcctg aaacgcagcc tgttaaaaac caaagaaaat
    44761 ctcggttccg gatttatcag cctgttccgc ggtaaaaaaa tcgacgatga tctgtttgag
    44821 gagctggaag agcagctttt gatcgccgat gtgggcgtgg aaaccacacg taaaattatc
    44881 accaatctga cggaaggcgc atcccgcaag cagcttcgtg atgccgaggc gctctatggc
    44941 ctgctgaaag aagagatggg tgagattctg gcgaaagtcg atgagccgct gaatgttgaa
    45001 ggtaaaacgc cgttcgtgat cctgatggtg ggcgtcaacg gtgtgggtaa aaccacgacg
    45061 attggtaagc tggcacgtca gtttgagcag cagggtaaat cggtgatgct ggcggcgggc
    45121 gatactttcc gtgcagcagc ggttgaacag cttcaggtct gggggcagcg caacaatatt
    45181 ccggtgattg cccagcatac cggggcggat tccgcctctg ttatcttcga cgccattcag
    45241 gcggctaaag cgcgtaatat cgacgtcctg attgccgata ccgccggacg cctgcagaac
    45301 aaatcgcacc tgatggaaga gttgaagaaa atcgtccgcg tgatgaagaa actcgacgtt
    45361 gaagcgccgc atgaagttat gctgactatc gatgccagca ccgggcagaa cgcggtaagc
    45421 caggccaaac tgttccatga agccgttggc ttaaccggca tcacgctaac gaaactggac
    45481 ggcacggcga aaggcggggt aattttctcg gtggctgacc agtttggtat ccctatccgc
    45541 tacattggtg tcggcgaacg tattgaggat ttgcgtccgt ttaaggcgga cgactttata
    45601 gaggcacttt ttgcccgaga ggattaacaa tgattcgctt tgaacatgtc agcaaggctt
    45661 atctcggtgg gagacaggcg ctgcagggcg ttacgttcca tatgcagccg ggtgagatgg
    45721 cgtttctgac cggtcattcc ggcgcaggga aaagtaccct cctgaagctg atctgtggga
    45781 ttgagcggcc cagcgccggg aaaatctggt ttagcggcca tgacatcacg cgtctgaaaa
    45841 accgtgaagt tccgtttctg cgccgccaga ttggcatgat tttccaggat caccatctac
    45901 tgatggaccg tactgtctac gataacgtgg cgatcccgct gattatcgcc ggtgccagcg
    45961 gtgacgatat tcgccgccgg gtgtcggcgg cactggataa agtcgggcta ctggacaaag
    46021 cgaagaactt ccctattcag ctttctggcg gtgaacaaca gcgtgttggc attgcccgcg
    46081 cggtggtgaa caagcccgcg gtactgctgg cggacgaacc gactggtaac ctggacgacg
    46141 cgttgtcgga agggattcta cgtctgtttg aagagtttaa ccgcgtaggg gtaaccgtat
    46201 tgatggcaac gcacgacatc aacctgattt cacggcgttc ctatcgcatg ctcaccctga
    46261 gcgatggtca cttgcatgga ggcgtgggcc atgaataagc gcgatgcaat caatcatatt
    46321 cggcagtttg gcgggcgtct tgatcgcttc cgtaaatcgg tcggcggctc aggcgacggc
    46381 ggtcgtaacg caccgaaacg cgcgaaatcc tcgccaaaac cggtaaatcg caaaaccaac
    46441 gttttcaacg aacaggtgcg ctatgccttc cacggcgcat tgcaggatct gaaaagcaaa
    46501 ccgttcgcca catttttaac ggtgatggtt atcgccatct ccctgacgct gcccagcgtc
    46561 tgttatatgg tgtacaaaaa cgttaaccag gcggcgacgc agtattatcc gtcaccgcaa
    46621 atcactgttt atctgcaaaa aacgctggac gatgacgccg ctgcgggcgt ggtggcacag
    46681 ttgcaggccg agcaaggcgt agagaaagtg aactatcttt ctcgtgaaga cgcactgggc
    46741 gagttccgta actggtctgg ttttggtggc gcgctggata tgctggaaga aaacccgctt
    46801 ccggcagtgg cggtggtgat cccgaagctc gactttcagg ggacggagtc tctgaatacg
    46861 ctgcgtgatc gtatcacgca gattaacggc attgacgaag tgcggatgga tgacagctgg
    46921 tttgcccgtc tggcggcgtt gaccgggctg gtcgggcgcg tttcggcgat gatcggcgtg
    46981 ttgatggtgg cggcagtgtt cctcgtcatc ggtaacagtg tacgcctgag tatctttgct
    47041 cgtcgtgact ccattaacgt gcagaaactg attggtgcaa cggatggttt catcctgcgc
    47101 ccgttcctgt acggtggcgc attgctgggg ttttctggcg cattgttgtc attaatttta
    47161 tcagaaattc tggtactgcg attgtcatcg gcggttgcgg aagtggcaca ggttttcgga
    47221 acgaagtttg atatcaatgg tttatcgttc gatgaatgcc tgctgttgct gctggtatgc
    47281 tcgatgattg gctgggtggc agcgtggctt gccacggtac aacatttacg ccactttacg
    47341 cctgaataat aaaagcgtgt tatactcttt ccctgcaatg ggttccgtag cagggaaaga
    47401 gaccccgttg tctcttcccg gtatttcatc tctatgtcac attttgtgcg taatttattc
    47461 acaagcctgc attgaacttg tggataaaat cacggtctga taaaacagtg aatgataacc
    47521 tcgttgctct taagctctgg cacagttgtt gctaccactg aagcgccaga agatatcgat
    47581 tgagaggatt tgaatgactg acaaaatgca aagtttagct ttagccccag ttggcaacct
    47641 ggattcctac atccgggcag ctaacgcgtg gccgatgttg tcggctgacg aggagcgggc
    47701 gctggctgaa aagctgcatt accatggcga tctggaagca gctaaaacgc tgatcctgtc
    47761 tcacctgcgg tttgttgttc atattgctcg taattatgcg ggctatggcc tgccacaggc
    47821 ggatttgatt caggaaggta acatcggcct gatgaaagca gtgcgccgtt ttaacccgga
    47881 agtgggtgtg cgcctggtct ccttcgccgt ccactggatc aaagcagaga tccacgaata
    47941 cgttctgcgt aactggcgta tcgtcaaagt tgcgaccacc aaagcgcagc gcaaactgtt
    48001 cttcaacctg cgtaaaacca agcagcgtct gggctggttt aaccaggatg aagtcgaaat
    48061 ggtggcccgt gaactgggcg taaccagcaa agacgtacgt gagatggaat cacgtatggc
    48121 ggcacaggac atgacctttg acctgtcttc cgacgacgat tccgacagcc aaccgatggc
    48181 accggtgctc tatctgcagg ataaatcatc taactttgcc gacggcattg aagatgataa
    48241 ctgggaagag caggcagcaa accgtctgac cgacgcgatg cagggtctgg acgaacgcag
    48301 ccaggacatc atccgtgcgc gctggctgga cgaagacaac aagtccacgt tgcaggaact
    48361 ggctgaccgt tacggtgttt ccgctgagcg tgtgcgccag ctggaaaaga acgcgatgaa
    48421 aaaactgcgc gctgctatag aagcgtaatt tccgctatta agcagagaac cctggatgcg
    48481 agtccggggt ttttgttttt tgggcctcta caataatcaa tttcccctcc ggcaaaacgc
    48541 caatccccac gcagattgtt aataaactgt caaaatagct attccaatat cataaaaatc
    48601 gggtatgttt tagcagagta tgctgctaaa gcacgggtag tcatgcataa aacgaaataa
    48661 agtgctgaac aacaacatca caacacacgt aataaccaga agaatgggga ttctcaggat
    48721 gaacataaag ggtaaagcgt tactggcagg atgtatcgcg ctggcattca gcaatatggc
    48781 tctggcagaa gatattaaag tcgcggtcgt gggcgcaatg tccggtccgg ttgcgcagta
    48841 cggtgaccag gagtttaccg gcgcagagca ggcggttgcg gatatcaacg ctaaaggcgg
    48901 cattaaaggc aacaaactgc aaatcgtaaa atatgacgat gcctgtgacc cgaaacaggc
    48961 ggttgcggtg gcgaacaaag tcgttaacga cggcattaaa tacgtgattg gtcacctctg
    49021 ttcttcatca acgcagcctg cgtctgacat ctacgaagac gaaggcattt taatgatcac
    49081 cccagcggca accgcgccgg agctgaccgc ccgtggctat cagctgatcc tgcgcaccac
    49141 cggcctggac tccgaccagg ggccgacggc ggcgaaatat attcttgaga aagtgaaacc
    49201 gcagcgtatt gctatcgttc acgacaaaca gcaatacggc gaaggtctgg cacgagcggt
    49261 gcaggacggc ctgaagaaag gcaatgctaa cgtggtgttc tttgatggta tcaccgccgg
    49321 ggaaaaagat ttctcaacgc tggtggcgcg tctgaaaaaa gagaatatcg acttcgttta
    49381 ctacggcggt tatcacccgg aaatggggca aatcctgcgt caggcacgcg cggcagggtt
    49441 gaaaactcag tttatggggc cggaaggcgt ggctaacgtc tcgctgtcta acattgcggg
    49501 cgaatcagcg gaagggctgc tggtgaccaa gccgaagaac tacgatcagg ttccggcgaa
    49561 caaacccatt gttgatgcga tcaaagcgaa aaaacaggac ccaagcggcg cattcgtttg
    49621 gaccacctac gccgcgctgc aatctttgca ggcgggcctg aatcagtctg acgatccggc
    49681 tgaaatcgcc aaatacctga aagcgaactc cgtggatacc gtaatgggac cgctgacctg
    49741 ggatgagaaa ggcgatctga aaggctttga gttcggtgtg tttgactggc acgccaacgg
    49801 cacggcaacc gatgcgaagt aacagataga taaatgaaaa tgcccgatgc gctgaactgc
    49861 catcgggttt ttttgtgttt gtcgaaatgg ttgtagaaag attaaacaat ccaggatatg
    49921 cttaatgtac ggtgacatac cgtacatatt atacggagca ggagagttca catgtctgct
    49981 gttaaaaagc agcgtatcga tctgcgttta actgacgatg acaaaagtat gattgaggaa
    50041 gcggcagcga tatctaatca gtccgtcagc caatttatgt tgaacagcgc ttcgcagcgg
    50101 gctgcggaag tgattgaaca gcatcggcgg gtgatcctca atgaggaatc ctggacgcgg
    50161 gtgatggatg cgctgagtaa tccaccgtca ccaggtgaaa agctaaaacg tgcggcaaaa
    50221 cgtcttcagg gaatgtaata atttatggat gatctgacga tagagattct gaccgatgat
    50281 gcagattatg atctacagcg attcgactgc ggcgaggaag cgttaaatct ctttctgacg
    50341 acacatctcg ttcgtcaaca tcgcaacaaa attctgcgag cgtatatcct ttgtcgcaac
    50401 actccagaac gtcaggtgct gggatattac acattatgcg gcagttgttt tgaacgagcc
    50461 gcattgccct cgaaatcgaa acagaaaaaa attccctaca aaaatattcc cagcgttact
    50521 cttgggcgtc tggcaattga tcgttcatta caggggcagg gatggggagc aacactggtt
    50581 gctcatgcca tgaaagtcgt ctggtcagcc tctttagcgg taggtattca cggtcttttt
    50641 gtcgaggcgc tgaatgaaaa agcccatacg ttttataaat cgctgggctt tatcccttta
    50701 gtcggggaaa acgaaaatgc gttatttttc ccaaccaaat ccattgaact gctttttaca
    50761 cagagcgatt aacgcttctc ccagccgccc tgctgtgccg taaatcccag tgcctgcata
    50821 aacgccgtca tcacaccgcg atcttccacg cctgcatccg ccatccacca gcatgaaacg
    50881 ccaggattgt tacgcaaaac ctcttccagc agatattgcc ccacaccgcg acgtcgagtg
    50941 acttcccgca cgcgcaggga atccagtgcc ccctcggtgc cgcttaaggt tacccgcacg
    51001 gcagcaagca ggcgctcgtt aaaacgcgcg gcgtagatgc ggtggttatc gtcaacctgt
    51061 aaagaggaag gggaatactc cggccagatc ttttgcaggt caatccggtc ctggtcgcta
    51121 aatttttcta atcgaatgat ggtcagcttc atgggtaacc cgtgtaaatc acaaaagtgg
    51181 aaccagtgta gcgaaataat ttaatcggag gctttctctt ttttatttct tttggcaggt
    51241 gattaatttc ttaacagcaa taattacaaa gttaaaacat tagagaatga aaaataccca
    51301 gcataatccc ctgaatggta gtgaattatt ccgccctttg tgccgttatt ttatgctgac
    51361 aaaggcactt ttttctgttt atctatcaat aaattcagaa tattatctgt tcttaatcga
    51421 ctgaaaaata gggactttaa tcgctattat cacaaaatac tgcgctaaac ccttaatcag
    51481 acaggcaaaa acagtgcagt ataaaaaaag aacagtctga tttgttaaca cataaaaaca
    51541 aagcaacaca acatcacgaa tggggatttt tgactatgaa acggaatgcg aaaactatca
    51601 tcgcagggat gattgcactg gcaatttcac acaccgcttt agccgacgat attaaagttg
    51661 ccgttgtcgg cgcgatgtcc ggcccgattg cccagtgggg cgatatggaa tttaacggcg
    51721 cgcgtcaggc gattaaagac attaatgcca aagggggaat taaaggcgat aagctggttg
    51781 gcgtagaata tgacgacgcc tgcgacccga aacaagccgt tgcggtcgcc aacaaaatcg
    51841 ttaatgacgg cattaaatac gttattggtc atctgtgttc ttcttctacc cagcctgcgt
    51901 cagatatcta tgaagacgaa ggtattctta tgatctcgcc gggagcgacc aacccggagc
    51961 tgacccaacg cggttatcaa cacattatgc gtactgccgg gctggactct tcccaggggc
    52021 caacggcggc aaaatacatt cttgagacgg tgaagcccca gcgcatcgcc atcattcacg
    52081 acaaacaaca gtatggcgaa gggctggcgc gttcggtgca ggacgggctg aaagcggcta
    52141 acgccaacgt cgtcttcttc gacggtatta ccgccgggga gaaagatttc tccgcgctga
    52201 tcgcccgcct gaaaaaagaa aacatcgact tcgtttacta cggcggttac tacccggaaa
    52261 tggggcagat gctgcgccag gcccgttccg ttggcctgaa aacccagttt atggggccgg
    52321 aaggtgtggg taatgcgtcg ttgtcgaaca ttgccggtga tgccgccgaa ggcatgttgg
    52381 tcactatgcc aaaacgctat gaccaggatc cggcaaacca gggcatcgtt gatgcgctga
    52441 aagcagacaa gaaagatccg tccgggcctt atgtctggat cacctacgcg gcggtgcaat
    52501 ctctggcaac tgcccttgaa cgtaccggca gcgatgagcc gctggcgctg gtgaaagatt
    52561 taaaagctaa cggtgcaaac accgtgattg ggccgctgaa ctgggatgaa aaaggcgatc
    52621 ttaagggatt tgattttggt gtcttccagt ggcacgccga cggttcatcc acggcagcca
    52681 agtgaaaatc ccaccgcccg tgaaatgcgg gcgggtttag aaaggttacc ttatgtctga
    52741 gcagtttttg tatttcttgc agcagatgtt taacggcgtc acgctgggca gtacttacgc
    52801 gctgattgcc atcggctaca ccatggttta cggcattatc ggcatgatca acttcgccca
    52861 cggcgaggtt tacatgatcg gcagctacgt ctcatttatg atcatcgccg cactgatgat
    52921 gatgggcatt gataccggct ggctgctggt agcggcggga ttcgtcggcg caatcgtcat
    52981 tgccagcgcc tacggctgga gtatcgaacg ggtggcttac cgcccggtgc gtaactctaa
    53041 gcgcctgatt gcactcatct cggcaatcgg tatgtccatc ttcctgcaaa actacgtcag
    53101 cctgactgaa ggttcgcgcg acgtggcgct gcccagcttg tttaatggtc agtgggtagt
    53161 gggtcatagc gaaaacttct ctgcctctat taccaccatg caggcggtga tctggattgt
    53221 caccttcctc gccatgctgg cgctgacgat tttcattcgt tattcccgta tggggcgcgc
    53281 ctgccgtgcc tgcgccgaag atctgaaaat ggcgagcctg cttggcatta acaccgaccg
    53341 ggtgattgct ttgacctttg tgattggcgc ggcgatggcg gcggtggcgg gcgtactgct
    53401 cggtcagttc tacggcgtca ttaaccccta tatcggcttt atggccggga tgaaagcctt
    53461 taccgctgcg gtgctcggtg ggattggcag cattccggga gcgatgattg gcgggctgat
    53521 tctggggatt gcggaggcgc tctcttctgc ctacctgagt acggaatata aagatgtggt
    53581 ctcattcgcc ctgctgattc tggtgctgct ggtgatgccg accggtattc tgggtcgccc
    53641 ggaggtagag aaagtatgaa accgatgcat attgcaatgg cgttgctctc tgccgcgatg
    53701 ttttttgtcc tggcgggcgt ctttatgggc gtacaactgg agctggatgg caccaaactg
    53761 gtggtcgaca cggcttcaga catccgttgg cagtgggtgt ttatcggcac ggcggtggtc
    53821 tttttcttcc agcttttgcg accggctttc cagaaagggt tgaaaagcgt ttccggaccg
    53881 aagtttattc tgcccgccat tgatggctcc acggtgaagc aaaagctgtt tctcgtggcg
    53941 ctgctggtgc ttgcggtggc gtggccgttt atggtctcac gcgggacggt ggatatcgcc
    54001 accctgacca tgatctacat tatcctcgga ctcgggctga acgtggtggt agggctttct
    54061 ggtctgctgg tgctggggta cggcggcttt tacgccatcg gcgcttatac ctttgcgctg
    54121 ctcaatcact attacggctt gggcttctgg acctgtctgc cgatagccgg gttgatggcg
    54181 gcggcggcgg gcttcctgct cggcttcccg gtgcttcgct tgcgcggtga ctatctggcg
    54241 atcgttaccc tcggtttcgg cgaaattgtg cgcatattgc tactcaataa caccgaaatt
    54301 accggcggtc cgaacggtat cagtcagatc ccgaaaccga cttttttcgg ccttgagttc
    54361 agccgtaccg ctcgtgaagg cggctgggac acgttcagta atttctttgg cctgaaatac
    54421 gatccctccg atcgtgtcat tttcctctac ctggtggcgt tgctgctggt ggtgctaagc
    54481 ctgtttgtca ttaaccgcct gctgcggatg ccgctgggtc gcgcctggga agcgttgcgt
    54541 gaagatgaaa tcgcctgccg ttcgctgggc ttaagcccgc gtcgtatcaa gctgactgcc
    54601 tttaccatca gcgccgcgtt tgccggtttt gccggaacgc tgtttgctgc gcgtcagggc
    54661 tttgtcagcc cggaatcctt cacctttgcc gaatcggcgt ttgtgctggc gatagtggtg
    54721 ctcggcggta tgggctcgca atttgcggta attctggcgg caattttgct ggtggtgtcg
    54781 cgcgagttga tgcgtgattt caacgaatac agcatgttaa tgctcggtgg tttgatggtg
    54841 ctgatgatga tctggcgtcc gcagggcttg ctgcccatga cgcgcccgca actgaagctg
    54901 aaaaacggcg cagcgaaagg agagcaggca tgagtcagcc attattatct gttaacggcc
    54961 tgatgatgcg cttcggcggc ctgctggctg tgaataacgt caatcttgaa ttgtacccgc
    55021 aggagatcgt ctcgttaatc ggtcctaacg gtgccggaaa aaccacggtc tttaactgcc
    55081 tgaccggatt ctacaaaccc accggcggca ctattttact gcgcgatcag catctggagg
    55141 gtttgccggg gcagcaaatc gcccgcatgg gcgtggtgcg taccttccag catgtgcgtc
    55201 tgttccgcga aatgacggta attgaaaacc tgctggtggc gcagcatcag caactgaaaa
    55261 ccgggctgtt ctctggcctg ttgaaaacgc cttctttccg tcgcgcccaa agtgaagcgc
    55321 tcgaccgcgc cgccacctgg cttgagcgca ttggtttgct ggaacacgcc aaccgtcagg
    55381 cgagtaacct ggcctacggt gaccagcgcc gtctggagat tgcccgctgc atggtgacgc
    55441 agccggagat tttaatgctc gacgaacctg cggcaggtct taacccgaaa gaaacgaaag
    55501 agctggatga gctgattgcc gaactgcgca atcatcacaa caccactatc ttgttgattg
    55561 aacacgatat gaagctggtg atgggaattt cggaccgaat ttacgtggtc aatcagggga
    55621 cgccgctggc aaacggtacg ccggagcaaa tccgtaataa cccggacgtg atccgtgcct
    55681 atttaggtga ggcataagat ggaaaaagtc atgttgtcct ttgacaaagt cagcgcccac
    55741 tacggcaaaa tccaggcgct gcatgaggtg agcctgcata tcaatcaggg cgagattgtc
    55801 acgctgattg gcgcgaacgg ggcggggaaa accaccttgc tcggcacgtt atgcggcgat
    55861 ccgcgcgcca ccagcgggcg aattgtgttt gatgataaag acattaccga ctggcagacg
    55921 gcgaaaatca tgcgcgaagc ggtagcgatt gtcccggaag ggcgtcgcgt cttctcgcgg
    55981 atgacggtgg aagagaacct ggcgatgggc ggcttttttg ccgaacgtga tcagtttcag
    56041 gagcgcataa agtgggtgta tgagctgttt ccacgtcttc atgagcgtcg tgttcagcgg
    56101 gcaggcacca tgtccggcgg tgaacagcag atgctggcga ttggtcgcgc gctgatgagc
    56161 aatccgcgtt tgttgttgct tgatgagcca tcgctcggtc ttgcgccgat tatcatccag
    56221 caaattttcg acaccatcga gcagctgcgc gagcagggga tgactatctt cctcgtcgag
    56281 cagaacgcca accaggcgct gaagctcgct gatcgcggct acgtgctgga aaacggccat
    56341 gttgtgctct ccgatactgg tgatgcgctg ctggcgaacg aagcagtgag aagtgcgtat
    56401 ttaggcgggt aaattatcga taacccggca gctaagttct gccgggttat tcgtccgtat
    56461 acagacttgt ttcaactgac cgggaggatt aaaaccagac cacttatttg ccacggaaag
    56521 gttccctccc atgcattatg ccgttgttgt catgcttgaa ctgctttgct gtattcacgc
    56581 ttaccgtacc ggacaagagc ggtactggat ttttatcatt ttctgttttc cggtgattgg
    56641 ctgcgtggcg tatttcgtca tagtgatgct gcctgaaacg ggcgctgacc gtcacgggca
    56701 tactttgctg atgcgcctgc aggacaaact caaccctgag cgccacctgc gcaaattaac
    56761 cgaagaactg gcgattgccg agacgaatca aaaccattat gcgttggcta atgaactggc
    56821 gcgtctgggg cgttatcacg aagctgttcc ccattatcag caggcgttaa gcgggatatt
    56881 cgcccatgaa gcggcaatga tgctaagcct ggcgcaggca cagtttgcta ttcaggagtt
    56941 cgccgcctgt cagcagacgc tggaagatgt gatgcgttat aaccctgatt ttcagtcggc
    57001 agacgggcat ttgttatttg ccagaacgtt agccgcacaa gaaaaatacg ctgatgcgga
    57061 aagcgagttt gaagtattga tttcttatta ccccggcccg caggcgcgta tttactatgc
    57121 ggaaatgttg gcgaaaatga gtcggttacg tgaagccaac gagcagtatg ttgccgtggt
    57181 tgatacggct aaacgctcaa gacctcatta tagaaaacac caccgggaat ggattaagac
    57241 cgccaatgag cggctgaagc agagtgtggt gcaatgaaaa tcttctttgc cgttagacca
    57301 gtgtcgcggc aatcttttgt taaaattgaa aaatgtagct ctcatcgggc agcaaaattg
    57361 gacgcagaat ttgctgccat ttttgctctt catggtaagg agttctggcg attagctggc
    57421 cgttaatcgc ctcttttcag tgcattctcg ggatatccgt gccccatctc ccattttccc
    57481 tcacccgtac cgtcaccgcc ttgtcatctt tctgacacct tactatctta caaatgtaac
    57541 aaaaaagtta tttttctgta attcgagcat gtcatgttac cccgcgagca taaaacgcgt
    57601 gaattcgcgc attcggtaca acaagagaga taaacgatga aaccgttaca ttatacagct
    57661 tcagcactgg cgctcggact ggcgttaatg gggaatgcac aggcagtgac gaccattccg
    57721 ttctggcatt ctatggaagg ggaactgggt aaagaggtgg attctctggc ccaacgtttt
    57781 aacgccgaaa acccggatta caaaattgta ccgacctata aaggcaatta cgaacagaat
    57841 ttaagcgcgg ggattgccgc atttcgtacc ggcaatgctc cggctatttt gcaggtttat
    57901 gaagttggca ccgccaccat gatggcgtcg aaagccatta aaccggttta tgacgtgttt
    57961 aaagaggcgg ggattcaatt cgatgagtcg cagtttgtgc cgacggtttc cggttactac
    58021 tccgacagca aaacgggcca cttactctcc cagccattca acagctcgac tcccgttctc
    58081 tattacaaca aagacgcctt caagaaagcg gggttagacc cggaacaacc gccgaaaacc
    58141 tggcaggatc tggcggacta tgccgcaaaa ctaaaagcct ccggcatgaa gtgcggctac
    58201 gccagcggct ggcagggctg gatccaactg gaaaacttta gcgcctggaa cggtctgccg
    58261 tttgccagca aaaacaacgg ctttgacggc acggatgcgg tgctggagtt caacaagccg
    58321 gagcaggtga aacacatcgc catgctcgaa gagatgaaca agaagggcga tttcagctac
    58381 gtcgggcgta aggatgaatc caccgagaag ttctataacg gtgattgcgc tatgaccacc
    58441 gcctcttccg gttctctcgc taatattcgc gagtacgcca aattcaacta tggcgtaggc
    58501 atgatgcctt acgacgccga tgcgaaagat gcaccgcaaa acgccattat cggcggggcc
    58561 agcctgtggg tgatgcaggg caaagataaa gaaacgtata ccggcgtggc gaagttcctc
    58621 gacttcctcg cgaagccaga aaacgctgcc gagtggcatc agaaaaccgg ttatctgcca
    58681 atcaccaaag cagcgtatga cctgacccgt gagcagggct tttatgagaa aaacccaggg
    58741 gcggataccg cgacgcgtca gatgctgaat aagccgccgt tgccgttcac caaagggctg
    58801 cgtctgggca acatgccgca gatccgcgtg attgtggatg aagagctgga gagcgtgtgg
    58861 accggtaaga agacaccaca gcaggcactg gataccgccg ttgagcgtgg aaatcagttg
    58921 ctgcgccgct ttgagaaatc gacgaagtct taatcagtgt aatgtcggat gcgtttcgct
    58981 tatctgacct ggcatcgcgt gtaggccgga taagcgaagc gcatccggca cagttcagga
    59041 attaaccgta atgtcatcat cccgtccggt gttccgctcg cgctggctgc cttatctgct
    59101 ggtcgcgccg cagctcatca tcaccgttat cttttttatc tggcctgcgg gcgaagcgtt
    59161 gtggtactcg ctgcaaagcg tcgatccgtt tggtttctcc agccggtttg tcggcctgga
    59221 taacttcgtc gcgctgtttc atgacagcta ctatatcgac tccttctgga cgacgataaa
    59281 attcagcacc tttgtcaccg tcagcggttt gctggtatcg ctgttctttg cggcgctggt
    59341 ggagtacatc gtgcgcggca gtcgtttcta tcaaaccttg atgctgctgc cgtatgccgt
    59401 ggctcccgcc gttgccgccg tattgtggat cttcctgttt aaccccggtc gcgggctgat
    59461 cacccatttt ctcgctgagt tcggctacga ctggaaccac gcgcaaaaca gcggtcaggc
    59521 aatgtttctg gtggtgtttg cctcagtatg gaagcaaatc agctacaact tcctgttctt
    59581 ctatgccgcg ctgcaatcca ttccccgttc gttgatcgaa gccgcagcca tcgacggtgc
    59641 agggccgatt cgccgcttct ttaagattgc gctgccgctt atcgccccgg tgagtttctt
    59701 cctgctggta gtgaacctgg tgtatgcctt cttcgacacc ttcccggtga tcgacgccgc
    59761 cacgtccggc gggccggttc aggccaccac tacgctgatt tataagatct accgcgaagg
    59821 ctttaccgga ctggatctgg cttcgtctgc cgcacagtcg gtggtgttga tgttcctcgt
    59881 catcgtgctg acggtgatgc agttccgcta tgttgaaagc aaggtgcgtt accaatgatt
    59941 gagaaccgtc cgtggctgac gatattcagc cataccatgc tgatcctcgg gatcgcggtg
    60001 atcctattcc cgctgtacgt ggcgtttgtc gcggcgacgc tggataaaca ggccgtctac
    60061 gccgcgccga tgacgctcat ccccggcacg catctgctgg aaaacatcca caacatctgg
    60121 gtaaacgggg taggcacaaa tagtgcgccg ttctggcgga tgttgcttaa cagcttcgtg
    60181 atggcgttca gcattacgct cggcaaaatt accgtctcga tgctctcggc attcgccatt
    60241 gtctggtttc gttttccgct gcgtaacctc tttttctgga tgatttttat caccctgatg
    60301 ttgccggttg aagtacgtat cttcccgacg gtggaagtca tcgccaacct gaagatgctc
    60361 gacagctacg caggtttaac gctgccgctg atggcctcgg cgaccgcgac gtttcttttc
    60421 cgccagttct ttatgaccct accggatgaa ctggtggaag ccgcgcggat cgacggcgcg
    60481 tcgccaatgc gcttcttttg cgacatcgtt tttccgttgt ccaaaactaa tctggcggcg
    60541 ttgtttgtga tcacctttat ttacggctgg aaccagtatt tgtggccgtt gttgattatt
    60601 accgacgtgg atctcggcac caccgtggca gggatcaaag ggatgatcgc cactggcgaa
    60661 ggcaccacag aatggaactc agtgatggcg gcgatgttgt taaccctgat cccaccagta
    60721 gtgattgttt tagtgatgca gcgtgccttc gtgcgcggcc tggtcgatag tgagaaataa
    60781 gatggcagga ctgaaattac aggcagtaac caaaagctgg gatggcaaaa cccaggtgat
    60841 taaaccgctg acccttgatg tggcggatgg cgaatttatc gtgatggtcg ggccgtcagg
    60901 ctgcgggaaa tcgacgctgc tgcgcatggt tgccgggctg gagcgggtga cggaaggcga
    60961 tatctgtatc aacgaccagc gggtgaccga aatggagccg aaagatcgcg ggattgcgat
    61021 ggtgttccag aactacgcgc tttatccgca tatgagtgtt gaagaaaaca tggcgtgggg
    61081 gctgaaaatt cgcggcatgg gcaagcagca aattgccgag cgcgttaaag aggcggcgcg
    61141 cattctggaa ctggacggtc tgcttaagcg ccgcccgcgc gagctttccg gcggtcagcg
    61201 tcagcgtgtg gcgatgggcc gggcgattgt gcgcgatccg gcggtgttcc tgtttgatga
    61261 gccactctct aacctcgatg ccaagctgcg cgtacagatg cgtcttgaac tgcaacagct
    61321 gcaccgtcgc ctgaaaacga cttcactcta cgttactcac gatcaggttg aggcgatgac
    61381 cctcgcccag cgagtaatgg tgatgaacgg cggcgttgcc gaacagattg gcacaccagt
    61441 tgaagtctac gaaaagcccg ccagcctgtt tgtggcgagt tttattggca gcccggcgat
    61501 gaatctgctg acaggccgcg tgaataacga aggcacgcac ttcgagctgg acggcggcat
    61561 tgcgctgccg ctaaacggtg gctaccgtca gtatgcaggg cgtaaaatga ctctcggcat
    61621 tcgcccggaa catatcgcgc taagctcgca ggcagaaggc ggcgtgccgc tggtgatgga
    61681 cacgctggag atcctcggcg cagataacct ggcgcacgga cgctggggcg aacagaagct
    61741 ggtggtacgg ctggcgcatc aggagcgccc gacggcaggc agcacgctgt ggctgcatct
    61801 gccggaaaat cagctacatc tttttgatgg tgaaacagga caacgagtat gagtaactgg
    61861 ccttatcccc gcatcgtcgc ccaccgtggc ggcggtaaac tggccccgga aaacaccctg
    61921 gcggcaatcg acgtcggggc aaaatacggt cataagatga tcgaatttga cgcgaagttg
    61981 tcgaaagatg gcgaaatctt cctgctccat gacgacaatc tcgaacgtac cagcaacggc
    62041 tggggcgtcg cgggtgaact gaactggcag gatttactac gtgtggatgc aggcagttgg
    62101 tacggcaaag cgtttaaagg tgagccgctg ccgttgcttt cgcaggtggc ggaacgctgt
    62161 cgcgaacatg ggatgatggc gaatatcgaa atcaaaccca ccactggcac tgggccatta
    62221 acgggcaaaa tggtggcgct ggcggcacgc gagctgtggg ccggtatgac gccgccgcta
    62281 ctgtcatcgt ttgagattga tgctttagaa gccgcacaac aggcagtacc agaactgccg
    62341 cgcggtttgt tgctggatga gtggcgcgac gactggcgag aactgaccgc gcggctgggc
    62401 tgcgtctcta ttcatctcaa tcataagttg ctcgataaag cgcgagtgat gcagttgaaa
    62461 gacgccggat tacggattct ggtttatacc gtcaataaac cccagcgcgc ggcagagttg
    62521 ctgcgctggg gcgtggattg catctgtacc gatgcgattg atgtgattgg tccgaacttt
    62581 acggcccaat agttttcaac ggtatatcag gctgcggcgt gccgggctga ttcagcatgt
    62641 cgccgtttct cctctccggc aacatatgct gctgattcaa cgaactatcc ggattacggt
    62701 tgctgtttaa catcccgcca ttggtgttgg gcagcatttg ctgccgggcg ggatttcgct
    62761 cccccggctg tgactgcaac acccgctgag aattattgtt tatctggttt tctaaatgct
    62821 gttgttgcaa ctgagtttgc gttttcagtt gctgattcag catccctttt tgctggattt
    62881 gctgcgtttg catctgggtt tgcatccgct gctgactggg gatctgatac cccggctggt
    62941 tagggttgtt cagagtatta atgggctgtg caaagccgac aaacggcagg agtgccgtaa
    63001 gaatcagaag tcgtttcatc gcgtatcctc ctctgaagat attctttaag tttactcctt
    63061 tcctgcaatc atcaggatta attcagagtt atataccagg cttagctggg gttgcccctt
    63121 aatctctgga gaataacgat gataaaaccg acgtttttac gccgggtggc cattgctgct
    63181 ctgttcacag gaagttgttt tagtacggta gccgcgccac ctgcgccgtc tcctgtttct
    63241 tacggcgtgg aggaagatgt ctttcatccg gtacgcgcga aacaggggat ggtggcatct
    63301 gtagatgcta ctgccactca ggtgggggtg gatatcctca aggagggcgg gaatgccgtt
    63361 gatgccgccg tggctgtggg ctacgcgctg gcggtaacgc atccgcaggc agggaatctg
    63421 ggcggtggtg gttttatgct gatccgctcg aaaaatggca ataccaccgc tatcgatttc
    63481 cgcgaaatgg cacccgccaa agcgacccgc gatatgttcc ttgatgacca gggcaacccg
    63541 gacagcaaaa aatcactcac ttcgcatctg gcttccggca cgcccggtac ggtcgctggt
    63601 ttctcgctgg cgttggataa atacggcaca atgccgttga acaaagtggt gcagccagcg
    63661 tttaaactgg cacgcgatgg ttttatcgtt aacgacgagc tggctgacga tctcaaaacc
    63721 tacgggagcg aagtgctgcc gaatcacgaa aacagcaagg ctatcttctg gaaagagggc
    63781 gagccgctga aaaagggcga caagctggtg caggcgaacc tggcaaagag cctggagatg
    63841 attgccgaaa acggcccgga cgaattctac aaaggcacga tagcggaaca gattgcccag
    63901 gagatgcaga aaaacggtgg cctgatcact aaagaagatt tagcggcgta taaagcggtc
    63961 gaacgcacac cgataagcgg cgattatcgc gggtatcagg tttactccat gccaccgcca
    64021 tcctccggcg ggatccatat cgtacaaatc ctcaatattc tggaaaactt cgatatgaag
    64081 aaatacggtt ttggcagcgc cgacgcgatg cagatcatgg cagaagcgga gaaatatgcc
    64141 tacgctgacc gctcggaata tcttggcgac ccggattttg tgaaagtccc gtggcaggcg
    64201 ctgaccaata aagcctatgc caaatctatt gccgagcaaa tcgatatcaa taaagcgaaa
    64261 ccgtccagcg agattcgccc cggcaagctt gcgccttatg agagtaatca aactacccat
    64321 tattcggtgg tggataaaga cggtaacgcg gtggcggtga cctatacgct gaacaccacc
    64381 ttcggtacgg gtattgtcgc gggcgagagc ggtattttgc tcaataacca aatggatgat
    64441 ttctctgcca aaccgggcgt accgaacgtt tacgggctgg tgggcggtga tgccaacgcc
    64501 gtcgggccga acaaacgccc gctgtcatcg atgtcaccaa ccattgtggt gaaagacggt
    64561 aaaacctggc tggtcaccgg tagccctggc gggagcctga tcatcactac agtgcttcaa
    64621 atggtggtga atagcatcga ttatggcatg aacgtcgccg aagcgaccaa tgcgccgcgt
    64681 ttccaccatc agtggttgcc ggacgagctg cgtgtcgaaa aagggtttag cccggatacg
    64741 ctcaagctgc tggaagcaaa aggtcagaaa gtggcgctga aagaggcgat gggcagtaca
    64801 caaagcatta tggttgggcc ggacggtgag ttgtacggcg catccgaccc gcgctcggtg
    64861 gatgatttaa cggcggggta ctaaggttag cggccctctt cgtgggaaga gggctgtttt
    64921 cttgaacaag ataaattagt gtctggattg cttttactat tgctccgtaa tatataatca
    64981 taattatcct gaatgctttt aaggggctta ggttatgctt tcttttcaag agtatgcaga
    65041 atagtctttc atttaccgtg aaaataggac gtgcgataat tacttcctg
//
LOCUS       DAZFAB010000015        64715 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZFAB010000015 DAZFAB010000000
VERSION     DAZFAB010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 64715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.015"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            177..458
                     /locus_tag="ACHJWA_001129"
     CDS             177..458
                     /locus_tag="ACHJWA_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4307775.1"
                     /translation="MTETELLRLIRRVAGINQQADEQATQPDSVTAENYVRVVAEVMH
                     RDGIQLNDADMRDIRIRVLEMLAYRRRIQTYREKAKITYQWKKPERLRR"
     gene            complement(582..881)
                     /locus_tag="ACHJWA_001130"
     CDS             complement(582..881)
                     /locus_tag="ACHJWA_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4307776.1"
                     /translation="MRMTRHKKQILELYKPEYRDWVRVEAGDPPFDVRGVTVLLYGSE
                     YQRYHIEATRRTLNAMVNDGILYRVKVREPRFDVRIGSDGAHCTVIRYGLGKSDD"
     gene            994..1296
                     /locus_tag="ACHJWA_001131"
     CDS             994..1296
                     /locus_tag="ACHJWA_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO4307777.1"
                     /translation="MVHDDKAEELEAKGLWRRAANRWGELLKQAASDEARQYVTERRT
                     ACIRKATITREPERDLICAIRTAATKTLTDMGIDLKKEDPLRGIQHGHTKRDKRRK"
     gene            2022..2342
                     /gene="clpS"
                     /locus_tag="ACHJWA_001132"
     CDS             2022..2342
                     /gene="clpS"
                     /locus_tag="ACHJWA_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFO4307778.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            2373..4649
                     /gene="clpA"
                     /locus_tag="ACHJWA_001133"
     CDS             2373..4649
                     /gene="clpA"
                     /locus_tag="ACHJWA_001133"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFO4307779.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(4995..5082)
                     /locus_tag="ACHJWA_001134"
     tRNA            complement(4995..5082)
                     /locus_tag="ACHJWA_001134"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5046..5048),aa:Ser,seq:gga)
     gene            complement(5336..5554)
                     /gene="infA"
                     /locus_tag="ACHJWA_001135"
     CDS             complement(5336..5554)
                     /gene="infA"
                     /locus_tag="ACHJWA_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFO4307780.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(5839..6543)
                     /gene="aat"
                     /locus_tag="ACHJWA_001136"
     CDS             complement(5839..6543)
                     /gene="aat"
                     /locus_tag="ACHJWA_001136"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFO4307781.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRNYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(6585..8306)
                     /gene="cydC"
                     /locus_tag="ACHJWA_001137"
     CDS             complement(6585..8306)
                     /gene="cydC"
                     /locus_tag="ACHJWA_001137"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFO4307782.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDS
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAVIL
                     RRVGLEKQLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(8307..10073)
                     /gene="cydD"
                     /locus_tag="ACHJWA_001138"
     CDS             complement(8307..10073)
                     /gene="cydD"
                     /locus_tag="ACHJWA_001138"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFO4307783.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGETELASTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(10196..11161)
                     /gene="trxB"
                     /locus_tag="ACHJWA_001139"
     CDS             complement(10196..11161)
                     /gene="trxB"
                     /locus_tag="ACHJWA_001139"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFO4307784.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            11706..12200
                     /gene="lrp"
                     /locus_tag="ACHJWA_001140"
     CDS             11706..12200
                     /gene="lrp"
                     /locus_tag="ACHJWA_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFO4307785.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            12335..16363
                     /gene="ftsK"
                     /locus_tag="ACHJWA_001141"
     CDS             12335..16363
                     /gene="ftsK"
                     /locus_tag="ACHJWA_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFO4307786.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDENIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQPQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLSPPPFD"
     gene            16518..17129
                     /gene="lolA"
                     /locus_tag="ACHJWA_001142"
     CDS             16518..17129
                     /gene="lolA"
                     /locus_tag="ACHJWA_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFO4307787.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            17140..18483
                     /gene="rarA"
                     /locus_tag="ACHJWA_001143"
                     /gene_synonym="mgsA"
     CDS             17140..18483
                     /gene="rarA"
                     /locus_tag="ACHJWA_001143"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFO4307788.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            18574..19866
                     /gene="serS"
                     /locus_tag="ACHJWA_001144"
     CDS             18574..19866
                     /gene="serS"
                     /locus_tag="ACHJWA_001144"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFO4307789.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            20105..22549
                     /gene="dmsA"
                     /locus_tag="ACHJWA_001145"
     CDS             20105..22549
                     /gene="dmsA"
                     /locus_tag="ACHJWA_001145"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFO4307790.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSGEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGIHNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            22560..23177
                     /gene="dmsB"
                     /locus_tag="ACHJWA_001146"
     CDS             22560..23177
                     /gene="dmsB"
                     /locus_tag="ACHJWA_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFO4307791.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLMPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            23179..24042
                     /gene="dmsC"
                     /locus_tag="ACHJWA_001147"
     CDS             23179..24042
                     /gene="dmsC"
                     /locus_tag="ACHJWA_001147"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFO4307792.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(24078..24704)
                     /gene="ycaC"
                     /locus_tag="ACHJWA_001148"
     CDS             complement(24078..24704)
                     /gene="ycaC"
                     /locus_tag="ACHJWA_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFO4307793.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            25019..26167
                     /gene="ycaD"
                     /locus_tag="ACHJWA_001149"
     CDS             25019..26167
                     /gene="ycaD"
                     /locus_tag="ACHJWA_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4307794.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            26377..27807
                     /gene="ycaM"
                     /locus_tag="ACHJWA_001150"
     CDS             26377..27807
                     /gene="ycaM"
                     /locus_tag="ACHJWA_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFO4307795.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYTPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(27808..27918)
                     /locus_tag="ACHJWA_001151"
                     /pseudo
     CDS             complement(27808..27918)
                     /locus_tag="ACHJWA_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(28017..28757)
                     /gene="pflA"
                     /locus_tag="ACHJWA_001152"
     CDS             complement(28017..28757)
                     /gene="pflA"
                     /locus_tag="ACHJWA_001152"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFO4307796.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(28949..31231)
                     /gene="pflB"
                     /locus_tag="ACHJWA_001153"
     CDS             complement(28949..31231)
                     /gene="pflB"
                     /locus_tag="ACHJWA_001153"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO4307797.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(31286..32143)
                     /gene="focA"
                     /locus_tag="ACHJWA_001154"
     CDS             complement(31286..32143)
                     /gene="focA"
                     /locus_tag="ACHJWA_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFO4307798.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            complement(32549..34309)
                     /gene="ycaO"
                     /locus_tag="ACHJWA_001155"
     CDS             complement(32549..34309)
                     /gene="ycaO"
                     /locus_tag="ACHJWA_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFO4307799.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDERLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            34439..35131
                     /gene="ycaP"
                     /locus_tag="ACHJWA_001156"
     CDS             34439..35131
                     /gene="ycaP"
                     /locus_tag="ACHJWA_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFO4307800.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKSGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            35330..36418
                     /gene="serC"
                     /locus_tag="ACHJWA_001157"
     CDS             35330..36418
                     /gene="serC"
                     /locus_tag="ACHJWA_001157"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFO4307801.1"
                     /translation="MAQIFNFSSGPAMLPVEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NVACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            36489..37772
                     /gene="aroA"
                     /locus_tag="ACHJWA_001158"
     CDS             36489..37772
                     /gene="aroA"
                     /locus_tag="ACHJWA_001158"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFO4307802.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            37941..38705
                     /gene="ycaL"
                     /locus_tag="ACHJWA_001159"
     CDS             37941..38705
                     /gene="ycaL"
                     /locus_tag="ACHJWA_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFO4307803.1"
                     /translation="MKNTKLLLAIVTSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            38878..39561
                     /gene="cmk"
                     /locus_tag="ACHJWA_001160"
     CDS             38878..39561
                     /gene="cmk"
                     /locus_tag="ACHJWA_001160"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFO4307804.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            39672..41345
                     /gene="rpsA"
                     /locus_tag="ACHJWA_001161"
     CDS             39672..41345
                     /gene="rpsA"
                     /locus_tag="ACHJWA_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFO4307805.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            41505..41789
                     /gene="ihfB"
                     /locus_tag="ACHJWA_001162"
     CDS             41505..41789
                     /gene="ihfB"
                     /locus_tag="ACHJWA_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFO4307806.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            41996..44260
                     /gene="ycaI"
                     /locus_tag="ACHJWA_001163"
     CDS             41996..44260
                     /gene="ycaI"
                     /locus_tag="ACHJWA_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HFO4307807.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILLAGETLTGATQDAIVEITATDGMTTHYGQITYLQ
                     GQRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRSGQIFLPGRWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVVILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILDQGLWFLADRSLALLFLGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSAHWPLQGRNDKGNNHSCVVK
                     VDDGTNSILLTGDIEAPAEQKMLSRYWQQVQATLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYTWLDTPHQGQVTVDFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            44297..46045
                     /gene="msbA"
                     /locus_tag="ACHJWA_001164"
     CDS             44297..46045
                     /gene="msbA"
                     /locus_tag="ACHJWA_001164"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFO4307808.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            46042..47028
                     /gene="lpxK"
                     /locus_tag="ACHJWA_001165"
     CDS             46042..47028
                     /gene="lpxK"
                     /locus_tag="ACHJWA_001165"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFO4307809.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     STGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLAQLTSLASGN"
     gene            47065..48297
                     /gene="ycaQ"
                     /locus_tag="ACHJWA_001166"
     CDS             47065..48297
                     /gene="ycaQ"
                     /locus_tag="ACHJWA_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFO4307810.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEYPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     QGIFREQWLADYYRLKRPALAAWREARAEQRQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTTLQKGLRQAITDFANWQQATRVTLGHC
                     PQGLFTDCRTGWEIDPVA"
     gene            48349..48531
                     /gene="ycaR"
                     /locus_tag="ACHJWA_001167"
     CDS             48349..48531
                     /gene="ycaR"
                     /locus_tag="ACHJWA_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFO4307811.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            48528..49274
                     /gene="kdsB"
                     /locus_tag="ACHJWA_001168"
     CDS             48528..49274
                     /gene="kdsB"
                     /locus_tag="ACHJWA_001168"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFO4307812.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAKGLETVGDNFLRHLGIYGYRAGFIRRYVTWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAHEVPGTGVDTPEDLERVRAEMR"
     gene            49428..50321
                     /gene="ycbJ"
                     /locus_tag="ACHJWA_001169"
     CDS             49428..50321
                     /gene="ycbJ"
                     /locus_tag="ACHJWA_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFO4307813.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(50298..51077)
                     /gene="elyC"
                     /locus_tag="ACHJWA_001170"
     CDS             complement(50298..51077)
                     /gene="elyC"
                     /locus_tag="ACHJWA_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFO4307814.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGSSGEPRQE"
     gene            51213..51998
                     /gene="cmoM"
                     /locus_tag="ACHJWA_001171"
     CDS             51213..51998
                     /gene="cmoM"
                     /locus_tag="ACHJWA_001171"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFO4307815.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPTQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            51995..53317
                     /gene="mukF"
                     /locus_tag="ACHJWA_001172"
     CDS             51995..53317
                     /gene="mukF"
                     /locus_tag="ACHJWA_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFO4307816.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALSYANADRLLDMRDEEMALR
                     DEEVTGELPPDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            53298..54002
                     /gene="mukE"
                     /locus_tag="ACHJWA_001173"
     CDS             53298..54002
                     /gene="mukE"
                     /locus_tag="ACHJWA_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFO4307817.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDIDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            54002..58462
                     /gene="mukB"
                     /locus_tag="ACHJWA_001174"
     CDS             54002..58462
                     /gene="mukB"
                     /locus_tag="ACHJWA_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFO4307818.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            58723..60570
                     /gene="ldtD"
                     /locus_tag="ACHJWA_001175"
     CDS             58723..60570
                     /gene="ldtD"
                     /locus_tag="ACHJWA_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO4307819.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGILPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQQALDKGQLPTFVAGLAPQHPQYA
                     VMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            60751..61299
                     /gene="mepK"
                     /locus_tag="ACHJWA_001176"
     CDS             60751..61299
                     /gene="mepK"
                     /locus_tag="ACHJWA_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFO4307820.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            61326..61973
                     /gene="gloC"
                     /locus_tag="ACHJWA_001177"
     CDS             61326..61973
                     /gene="gloC"
                     /locus_tag="ACHJWA_001177"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFO4307821.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDA
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(62024..63214)
                     /gene="aspC"
                     /locus_tag="ACHJWA_001178"
     CDS             complement(62024..63214)
                     /gene="aspC"
                     /locus_tag="ACHJWA_001178"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFO4307822.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRIAADFLAKNTSVKRVWVSNPSWPNHKSVFNSADLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(63399..64487)
                     /gene="ompF"
                     /locus_tag="ACHJWA_001179"
     CDS             complement(63399..64487)
                     /gene="ompF"
                     /locus_tag="ACHJWA_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFO4307823.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQLLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNISGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT        16010 a        15729 c        17187 g        15789 t
ORIGIN      
        1 tgcgcatttt tgtgatttta tcgtcatcat catcatgtat gtaacctgtt gtttttaatg
       61 taatcgatgc aaaaaagatg atggagaagg ttgttttttg ttcgacatct tttagcgtga
      121 cagattcttt acaaaaaatc tgagcttgtt ttcttaacca gcgcgatggg ggcacaatga
      181 cagaaaccga actactgcga ttaatccgcc gcgtcgccgg aatcaaccag caggctgacg
      241 aacaggccac gcagccggac agcgtgaccg cagaaaatta tgttcgtgtg gttgctgagg
      301 tgatgcaccg tgatggtatc cagcttaatg atgcggatat gcgcgacata cggatccgcg
      361 ttcttgaaat gctggcctac cgtcgccgga tacagacgta cagggaaaaa gccaaaataa
      421 cgtaccagtg gaagaagccg gagcggttac ggcggtaact tgctgaagtt aaagcataat
      481 gcaaatttgc actctggcct aacccgttaa tatttcagca aagtgcaaat ttgcgttctg
      541 tagctggtgg ctggcagaat gtaaatttac attctggtga tttaatcatc tgatttaccc
      601 agcccgtacc ggattaccgt acagtgagct ccatcgctac cgatacgcac atcaaagcgg
      661 ggttcgcgca ctttcacgcg gtacaggatc ccatcattta ccatagcgtt taacgttcgt
      721 cgggttgcct ctatgtgata tcgctgatat tcggaaccat agagcagcac tgtcacacca
      781 cgaacatcaa aaggcggatc tccggcctcc acccgcaccc aatcgcgata ctcaggtttg
      841 taaagttcaa gaatctgttt tttgtgcctg gtcattctca tgtgtgattc cttattgtgg
      901 gacagtactg tcatacaata agctatgagg gaaattatca aaagaacgga aaccaaccat
      961 tcagatttaa caggtatcaa tgggattatg cttatggtgc atgatgacaa agcggaagaa
     1021 ctggaagcga aaggtttatg gcgacgggcg gctaatcgct ggggtgagtt actgaaacag
     1081 gcggcaagtg atgaagcgcg tcagtatgta acggaacgac gaacggcatg tatccggaag
     1141 gcaaccataa cccgggaacc ggaaagagac ctgatttgtg ccataagaac cgccgccaca
     1201 aaaacactaa cggacatggg gatcgacctg aaaaaagaag atccactgcg gggcattcag
     1261 cacggacata caaagcggga taaacgcagg aagtaatttt tttcgtgtaa ccaggcaacc
     1321 gactatctga aagaagtcag aactacgaaa ttttcgtagt ttaatttgca gccaggctat
     1381 cggagatcgt gacggagagt gacaaccagt gacacgacgt gacaaaaaga cggtttaacc
     1441 gttaattaaa aatcgatggg attttgtgct tttcggacga gtggccaacg tcatttttag
     1501 cgaaaagctc tgattaaggt cattggctga atggtcataa tgaccacgca gatcatcaag
     1561 gcaaccataa cgacttccgt tacggttgat gcttttaccc cattggggga aaggtattac
     1621 gataatcata acacctaccg aacaggtaaa gcccaccagc ctgattaaca gttaaccgga
     1681 aaaaaatccg gtatgggggg agtgggtagg tggggatccc catatcgggg aacatccacc
     1741 agcttcataa cggatggccg gaaaatgata acggttaaag cgtgttgatc tttgttcgtg
     1801 gttgttcgat attgttcgtg aaacggtgta gttaatggtg tagttaattt cgctgttttt
     1861 gacagagttg ttatttgtaa cacagtgaaa aataaagact aatcaatgaa atgttataaa
     1921 tgatagttat ctatcatgtg gagtagattg gtcaggcaaa taagctcttg tcagcggcag
     1981 ggcgttctgc cgataaccgt aaccgaagat gataactgac aatgggtaaa acgaacgact
     2041 ggctggactt tgatcaactg gcggaagaaa aagttcgcga cgcgctaaaa ccgccatcta
     2101 tgtataaagt gatattagtc aatgatgatt acactccgat ggagtttgtt attgacgtgt
     2161 tacaaaaatt cttttcttat gatgtagaac gtgcaacgca attgatgctc gctgttcact
     2221 accaggggaa ggccatttgc ggagtcttta ccgccgaggt tgcagaaacc aaagtggcga
     2281 tggtgaacaa gtacgcgagg gagaatgagc atccattgct gtgtacgcta gaaaaagcct
     2341 gaatgcaggc ataaaaattg ggggaggtgc ctatgctcaa tcaagaactg gaactcagtt
     2401 taaatatggc tttcgccaga gcgcgcgagc accgtcatga gtttatgacc gtcgagcact
     2461 tgttactggc gctgctcagt aacccatctg cccgggaggc gctggaagcg tgttctgtgg
     2521 atttggttgc gctccgtcag gaactggaag cctttattga acaaaccaca cccgttctgc
     2581 ctgccagtga agaggagcgc gacacacagc cgacgctgag ttttcagcgt gtactgcaac
     2641 gtgcggtctt ccatgtccag tcctccggtc gcaatgaggt taccggtgca aacgttctgg
     2701 tcgctatctt tagcgaacag gagtcgcagg cggcatatct gttgcgtaaa catgaagtca
     2761 gccgtctcga tgtggtgaac tttatctctc atggcacgcg taaagacgag ccgacacagt
     2821 cttctgatcc tggcagccag ccaaacagcg aagaacaagc tggtggggag gaacgtatgg
     2881 agaatttcac gacgaacctg aatcagcttg cgcgcgtggg cggaatcgac ccactgattg
     2941 gtcgtgagaa ggagctggag cgtgctattc aggttctctg ccgtcgccgt aaaaacaacc
     3001 cgctgctagt gggggaatct ggtgtcggta aaaccgcgat tgcagaaggt cttgcctggc
     3061 gaattgttca gggcgatgtg ccggaagtga tggctgactg tacgatttac tctctcgata
     3121 tcggttctct gttagcgggc acaaaatatc gcggcgactt tgaaaaacgt tttaaagcgt
     3181 tgctcaagca gctggagcag gacactaaca gcatcctgtt tattgatgag atccacacca
     3241 ttatcggtgc gggtgcagcg tctggtggtc aggttgatgc ggctaaccta atcaaaccgt
     3301 tgctctccag cggtaaaatt cgcgtaattg gttcgacaac ctatcaggag ttcagcaaca
     3361 ttttcgagaa agaccgtgct ctggcgcgtc gcttccagaa aattgatatt actgaaccgt
     3421 cgatcgaaga aactgttcaa atcatcaatg gcctgaaacc gaagtatgaa gcacaccacg
     3481 acgtgcgtta taccgcaaaa gcggtgcgtg cggcggtaga gctggcggtg aaatacatta
     3541 acgatcgtca tctgccggat aaagccattg atgttatcga cgaagcgggc gctcgcgcac
     3601 gtctgatgcc ggtaagcaaa cgcaagaaaa ccgttaatgt ggcggatatt gagtccgtgg
     3661 tggcccgtat tgcacgcatt ccagagaaga gtgtttctca gagtgaccgc gataccctga
     3721 aaaacctcgg cgatcgcctg aaaatgctgg tcttcggtca ggataaagcc attgaggcgc
     3781 tgactgaagc cattaagatg gcgcgtgcag gtttaggtca cgaacataaa ccggtcggtt
     3841 cgttcctgtt tgccggccct accggggtcg ggaaaacaga ggtgacggta cagctttcga
     3901 aagctttggg cattgagctt ctgcgctttg atatgtccga gtatatggaa cgccataccg
     3961 tcagccgtct tattggtgcg cctccgggat acgttggttt tgatcagggc ggtttgctga
     4021 ctgatgcggt catcaagcat ccacatgcgg tgctgctgct ggacgaaatc gagaaagcgc
     4081 acccggacgt gttcaatatt ctgttgcagg tgatggacaa cggtacgctg accgataaca
     4141 acggacgcaa agcggacttc cgtaacgtgg tactggtgat gaccactaac gccggggtac
     4201 gggaaactga gcgcaaatcc attggtctta tccaccagga taacagcacc gatgcgatgg
     4261 aagagatcaa gaagatcttt acaccggaat tccgtaaccg tctcgacaac attatctggt
     4321 tcgatcatct gtcaactgac gtgatccatc aggtggtgga taaattcatc gtcgagttgc
     4381 aggttcagct ggatcagaaa ggtgtttctc tggaagtgag ccaggaagcg cgtaactggc
     4441 tggccgagaa aggttacgac cgggcaatgg gcgcacgtcc gatggcgcgt gtcatccagg
     4501 acaacctgaa aaaaccgctc gccaacgaac tgctgtttgg ttcgctggtg gacggcggtc
     4561 aggtcaccgt cgcgctggat aaagagaaaa atgagctgac ttacggattc cagagtgcac
     4621 aaaagcacaa ggcggaagca gcgcattaat ctgattgtca ggtaggttgg tgaagtccgt
     4681 aatctcgaaa gaggttacgg actttttatt tatggggtgg aacggtgaaa accctatttt
     4741 tggaggtgaa ggtaatttgt tgataattag tgctgctgga aaaggtaagg ataaaaaagg
     4801 gtgacagcag gaaaatgaga tggttttgct ttattaacaa cgggctaaac gtgtagtatt
     4861 tgagttcact gccgtacagg cagcttagaa attcactggt aacatactcc acccgcccac
     4921 catgttcact gccgtacagg cagataaaat gcgaaaaaaa agcccgtact ttcgtacgag
     4981 ctcttcttta aatatggcgg tgaggggggg attcgaaccc ccgatacgtt gccgtataca
     5041 cactttccag gcgtgctcct tcagccactc ggacacctca ccaaattgtt ttgctgccaa
     5101 acctcatggg tggcaacggg gcgctactat agggagttgg agtaaaacgg tcaagaagaa
     5161 ttttaatgat aattattgtt tgctcatact gtaaacaact tgtgcagtat atctacatcg
     5221 agacaagtta tggacttata cttccaaagt acttcatgca tatcacaaaa taaaaaggcc
     5281 ggttaaaccg accttttact cgttctttct cttcgcccat caggcggtaa aacaatcagc
     5341 gactacggaa gacaatgcgg cctttgctca ggtcgtacgg ggtcagttca acagtcactt
     5401 tgtcgcccgt caggatgcgg atgtagtttt tgcgcatttt accggagatg tgtgcagtaa
     5461 ccacgtgacc gttttctaac tctacgcgga acatggtatt aggcaacgtt tcaagaacgg
     5521 taccttgcat ttcaatattg tcttctttgg ccatctaatc ctctggggta tcactaccgt
     5581 aatttgaacc ggcaagataa tgccgaagtt ctgtaaataa gtaaagattt gcgcgctaaa
     5641 tcgcaacaaa caggttcggc acattactcc gaaaacacac ggctaagccg caccaaaagc
     5701 gcaacgtata agggagcggt gagataaacg atgggcgtta cctgacgcga aaaattcctt
     5761 atcggcagcg gggtaatgag cgtaaccaac tctgcgaccg caattataac actctgggga
     5821 gaaatgtgcc gaaaacattc attcttgtgg tgaaaacaag catcgtggta cccagaaatt
     5881 attcggcaat cgtccgaggc gcatttgatt gagataatta aggtaattcc ggcggggaat
     5941 ttcgcaggca ccaagcgatg ctgtgtgatc gttaaggacc tggcagtcga taagcttacc
     6001 gccatgaccg ataaattcct cacagaatac cagaagcgcc gttttagacg cattttccat
     6061 ccggctgaac atggactcgc cacaaaatag cgttccctgg gccacgccgt acataccgcc
     6121 gacaagctca tcttcacgcc agacttcaat ggagtgggca tgaccgagtt cgtgaaggcg
     6181 atggtaggct tcgaccacgc cacgcgtgat ccaggttcct tcttcgcgat cgctggcaca
     6241 gccttcaatg acctgaccaa aagcgtaatt catcgtgaca cgatagggcg agcgtttatg
     6301 aaatcgcttc atactacggc tgatatgcag tgattctggc catagcaccg cgcggggatc
     6361 gggcgaccac cagaggatgg ggtcgcctgg agaaaaccac ggaaaaatac cacgctggta
     6421 agccattaac aggcgcgcag ggctaagatc gcccccaagt gccagcaggc cgttaggctc
     6481 acgtaatgcg ccttccgggg aagggaaggc tattgaatgg cgagaaagct gaaccaggcg
     6541 catgaccgca aaactccacg caagtcggat cgttcaataa tagcttacaa accctgcttg
     6601 aactggtaat aacgcccctg tctggcaagc agttctgcgt gagtaccttg ctcaataatt
     6661 tgcccgttgt ccatcactat tatttgttgg aaacgagaga gtccgcgaag tcgatgggtg
     6721 accattaaca ccgttttctc acgcatcatt tctgcaagca attcaaggat ctggctttcg
     6781 gttgtggcat ctaagccttc ggtaggttca tccagcaaca ccagtggcgc atcatgtaac
     6841 agcgcacggg cgatagccag acggcgcagt tcaccaccgg agagctggcg tccgccttca
     6901 cctaaccaac tgttgagacc tgcatcctcg agctgcttct ccagaccaac gcgacgtaag
     6961 atcaccgcca gagcctcatc actactgcca ggcgaggcga gtaacagatt atcacgcagc
     7021 gtggcgctaa acagatgcac tcgctgagga acaacgctga tggtctgtcg tagagccgct
     7081 tcattcaggc tggctatggg gctatcgtta agcaaaatct cgccctgttg cgggtcccat
     7141 gcgcgggtca gcagttgtaa cagtgttgat ttgccgcatc cggttcgccc gagaatcgct
     7201 atatgttccc cggcgtttac ctgaagagaa atccctttaa gtgcctgttg ggattgctcc
     7261 ggataagtga actgaacatc ccgtaatgtc agcgaaacgc gatcggcaac acgagtttgg
     7321 gtatcaggga aggtgacctc cggtttttga tccgttaagt cagagatacg tacggcagag
     7381 gcaatgactt gccccagatg ctgaaatgca cccgttactg gtgccaatgc ttcaaacgcg
     7441 gctaacgcgc aaaagacaaa cagggcaatt aacgcgccgg gttgagcatt gccgccaacg
     7501 ccgccagacg ccatccacag catcaggatc accgctaacg cgccaatgag cagcattatc
     7561 gcttgcgaca atgcggtcag ttcagattga cggcgttgcg cttccagcca ttgaatttct
     7621 gtattctcta gttgcgtgcg ataacgatcg ctggcaccaa aaatggtcag ctcagcttgc
     7681 ccttgcagcc aggccgtcag ttgttggcga tactgtccgc gaagatgagt cagattttgc
     7741 ccggtgcttt ttcccgcacg ataaaacagc ggtggcatca ggaaaagcgt cagtaacata
     7801 atgccgccca gcgtaaaggc gagggtggaa tcaaggaaac ttaacccgat tgtcaccacc
     7861 ataatcacca caaaagcgcc caccagcggc gagataacgc gcaggtaaag atgatcgagc
     7921 gtatcaacat ccgccaccac gcggttgagc aattcgccct gacgatagcg cgccagtccg
     7981 gcaggggaga ggggcaacaa tttgctgaag gtgtaaatgc gcagatgctg caacacgcgg
     8041 aaagtcgcgt cgtgacttac cagacgttca aaataacgcc cggcagtacg ggtgattgct
     8101 gcaccacgca cgcccgcagc gggtagcata tagttgaagc tgtacagtcc ggcaaccccc
     8161 gcaaccgctg aggccgagag gaaccagccg gaaagtgtca acagaccgat actggcgagc
     8221 agcgtcacaa ttgccagcac aataccaaga cttaacatcc atttatgacg tttatacagt
     8281 gccagatagg gtagcaaagc gcgcatttaa atctcctcct gacgatgggc cagtaatgtg
     8341 gcgaatgggc caccagccac acttaattcc gcgtaacgtc cttgctcaat aatccggcca
     8401 tcctgcataa cccaaatgac atcccagtca gcaagatctt ctaactggtg ggtgaccatt
     8461 aacgttgtct ggcgcagaga ggcggcattc agcgcctcca ttacgcgctg ttcactgtga
     8521 gcatcaaggc tggcagcggg ttcatccaac agtaatagcg aacagggatt tagtaacgca
     8581 cgggccaccg ccacgcgctg cgcctgcccc acggaaaggc gggcagcctg gtcgccaaca
     8641 ggcgtatcaa cgccttgtgg caggagcggt agaaactcgc tgacccaggc gttatccagc
     8701 gctgcttgta attcttgttc gctggcatca ggtcgcgcca gtagtacgtt atcccgtaat
     8761 gttgctgccg gtaattgtgg gttttgacca acccaggaga gatgtttacg ccatgattct
     8821 ggtgataaat cgcgtaattc tatcccgttg attcgtaatg atccctgata tgagagaaaa
     8881 ccagaaagcg cgttcagcag tgagctttta cctgaaccgc tgcgaccaac caacactgca
     8941 cgttggcctg ctggcaaagt aaagttcagc ggtccggcca gcgttttacc ttccggcgac
     9001 gtgataaaca gatcctcggc ctcaatggtc agcggatcgg ttgatgctaa ttccgtctca
     9061 ccgcgctgcg gatgggcgag cggggtttcc ataaacgttt tcagactgtc agctgcacca
     9121 acagcctggg ctttagcatg ataaaacgta ccgagatcgc gtaatggctg gaaaaactct
     9181 ggcgcaagga tcagggccag aaaacccgca gccagcgtca cgccagtatc gtagtgacca
     9241 aaatccagct cgccgagata ggaaaaacca aagtagaccg ccaccagagc aattgacagc
     9301 gaggtaaaaa attcgagaat gccggaggat aaaaacgcca gccgtagcac ttccattgtc
     9361 cgttggcgga aatcttccga agcagaacga atactttcaa tttcagcttc accacgacca
     9421 aaaatacgca atgtttccat gccgcgcagg cgatcgagga aatgcccact taagcgagca
     9481 agagcgagaa agttacgtcg gttagcatcg gcagccccca tcccaaccag cgccataaac
     9541 aacggaatta acggtgcagt gcccagcaga atgagcgccg cagcccagtt agaggggaag
     9601 atcgccacca caatcagcaa cggcaccgac actgccagcg ccatttgcgg caggtagcgt
     9661 gcatagtaat catgcatatc gtcaatttgc tcgagtacca gcgtcgccca gctccccgca
     9721 ggtttaccct gaatccacgc tggccctgct tgttgcagac ggtcgagaac ctgacggcgg
     9781 atggcaaagc ggatatgctg cccggcgtga taacccaccc gttcgcgtaa ccagaccacc
     9841 catgcgcgca gtacaaaggt cagaaccagt aacgtaaagg gaagcagcag ggcttcacgg
     9901 ggaatattct ccataatcat atgttgcaga attcgcgcca tgaaccatgc ctgggcaatg
     9961 atcaatatgc cgctcacaaa gcccagcaga cgagaaatat tcagccaacg ttgggagatg
    10021 acgctttgct gttttaacca gcgggttaac tctttttgac gagatttatt cattgcacgc
    10081 ttagcaggtg agttatcaga attatttgca gagcaatgtt acaacgggga aaaaataaag
    10141 gcgacccata gtcgcatggt gtcgccttct ttacttttgt tactgatttg taaaattatt
    10201 ttgcgtcagc taaaccatcg aggtagcgtt ccgcatcaag tgctgccatg cagcctgtac
    10261 cagcagatgt aatggcctgg cgataaatgt gatccatcac gtcgcctgcg gcaaagacgc
    10321 caggaatgct ggtctgggtg gcattaccat gaatacccga ctgcactttg atgtagccgt
    10381 tttccagttc cagctgccct tcgaaaatgg cagtattcgg gctgtgaccg atagcaacaa
    10441 acagaccggc aacgtcgagt gactcgatgt tatcgctgtt ttgcgtatcg cgcagacgaa
    10501 cgccagtgac acccatttga tcgccggtca cttcttccag cgtacggttg gtgtgcagaa
    10561 tgatgttgcc gttctccact ttatccatca gacgcttaat gaggattttt tccgcgcgga
    10621 aaccgtcacg gcggtgaatc agatgcactt ccgaagcgat gttagacaga tacagcgcct
    10681 cttcaaccgc ggtattgccg ccgccgatga ccgcaacttt ctggttgcga tagaagaaac
    10741 cgtcgcaggt tgcacaagca gaaactccac ggcctttaaa ggcttcttca gagggcaggc
    10801 cgagatagcg tgcagaagct ccggtggcaa taatcagcgc gtcgcaagtg tattcgccgt
    10861 tatcgccatt cagacggaac ggacggtttt gcagatccac cttgttgata tgatcaaaaa
    10921 tgatctcagt ttcaaacttg gtggcatgtt cgtgcatgcg ctccattaat aacggaccgg
    10981 tcagatcgtt tggatcgcca ggccagtttt ccacttccgt ggtggtggtc agttggccgc
    11041 ctttttccat gccggtaatc agcacaggtt gcaggttggc gcgcgccgca tagacagcag
    11101 cggtgtatcc cgccgggcct gaacccagga taagcagttt actgtgtttg gtcgtgccca
    11161 tgagatcccc atagttgttg gcagacaatg ggcaggattg tagggaattt acagacgtaa
    11221 aaaaagagta tgacgatttt gttaacaatt tgtgcaatcg gcagcatcga taagcaggtc
    11281 aaattctccc gtcattatca cctctgctac ttaaatttcc cgctttataa gccgattaaa
    11341 tgatgaataa acgcccctgt taatgaatat ctggcatgtt gtactaaaaa tcgatgtttt
    11401 gctttgacaa tcccctggtg ttttgcgaaa acattcgagg aagaaaaaaa acagtattct
    11461 tatatgcgca taaccatgca tgtaaatacc atgtttaccg tgctagtgaa atctacgtat
    11521 ggcgtggaca gacgccattc gtgatgtcga tagctgccac aaggcaacgg tcttctcacc
    11581 gtagacccag gcattgcgcg ccgtgaatct tcatgatttc ggtctatcgt gacgggtagc
    11641 gactctgaac agtgatgttt cagggtcaga caggagtagg gaaggaatac agagagacaa
    11701 taataatggt agatagcaag aagcgccctg gcaaagatct cgaccgtatc gatcgtaaca
    11761 ttcttaatga gttgcaaaag gatgggcgta tttctaacgt cgagctttct aaacgtgtgg
    11821 gactttcccc aacgccgtgc cttgagcgtg tgcgtcggct ggaaagacaa gggtttattc
    11881 agggctatac ggcgctgctt aacccccatt atctggatgc atcacttctg gtattcgttg
    11941 agattactct gaatcgtggc gcaccggatg tgtttgaaca attcaatacc gctgtacaaa
    12001 aacttgaaga aattcaggag tgtcatttag tatctggtga tttcgactac ctgttgaaaa
    12061 cacgcgtgcc ggatatgtca gcctaccgta agttgctggg ggaaaccctg ctgcgtctgc
    12121 ctggcgtcaa tgacacacgg acatacgttg ttatggaaga agtcaagcag agtaatcgtc
    12181 tggttattaa gacgcgctaa cacggaacag gtgcaaaatc ggcgtatttt gattacactc
    12241 ctgttaatcc atacagcaac agtactgggg taacctggta ctgttgtccg ttttagcatc
    12301 gggcaggaaa agcctgtaac ctggagagcc tttcttgagc caggaataca ctgaagacaa
    12361 agaagtcaca ttgacaaagt taagtagcgg ccgccgcctt ctggaggcgt tgctgatcct
    12421 tattgtcctg tttgccgtct ggttgatggc tgccttacta agctttaacc cttcggaccc
    12481 cagctggtcg caaacggcct ggcatgaacc tatccataat ttaggtggga tgcccggtgc
    12541 gtggttggca gatacgctgt tctttatttt tggcgtgatg gcttacacca ttcccgtcat
    12601 tattgtcggc ggttgttggt ttgcctggcg tcatcagtcc agcgacgaaa acattgatta
    12661 ttttgccgtt tcgctacgca tcattggcgt tttggcgctc atccttacct cctgtggtct
    12721 ggcggcaatc aacgctgacg atatctggta ttttgcctcc ggtggcgtca ttggcagctt
    12781 actaagcact acgctacaac cactgctaca cagtagcggg ggaactattg cgctgctctg
    12841 cgtttgggca gcgggcctga cgttgttcac cggttggtca tgggtgacca ttgctgaaaa
    12901 actcggcggc tggattttaa acattctcac cttcgccagt aatcgtaccc gtcgcgatga
    12961 tacctgggtc gatgaagatg agtatgaaga cgacgaagag tatgaagatg aaaaccacgg
    13021 caaacagcat gaatcacgcc gtgcccgtat tcttcgcggc gcgctagcgc gtcgtaaacg
    13081 gttggcggaa aaattcatta atccgatggg gcggcaaaca gacgctgcgt tgttctccgg
    13141 taagcggatg gatgatgacg aagagattac ctacactgca cgcggtgtgg ctgccgaccc
    13201 ggacgacgtc ctattttcgg gcaatcgtgc aacgcagcca gaatatgacg aatacgatcc
    13261 attattaaac ggtgcgccaa ttaccgaacc tgtcgctgta gcagctgctg ctaccacggc
    13321 gacacaaagc tgggctgcgc cggttgaacc tgtgactcag acgccgcctg ttgcctctgt
    13381 tgatgttcca ccttcgcaac ctacagtagc ctggcagcct gtaccgggtc cacaaacggg
    13441 agagccggtt attgctcctg caccggaagg ttacccacag cagtcacaat atgcgcagcc
    13501 tgcagtgcaa tataatgagc cgctgcaaca accagtacag ccgcagcagc cgtattatgc
    13561 acctgcagct gaacaacctg cgcaacagcc gtattatgcc cctgcgccag aacaaccggt
    13621 ggcaggtaac gcctggcaag ccgaagagca gcaatccact tttgctccac agtctacata
    13681 ccagactgag caaacttatc agcagccagc cgctcaggag ccgttgtacc aacagccgca
    13741 acccgttgaa cagcagcctg ttgtggagcc tgaacccgtt gtagaagaga caaaacccgc
    13801 gcgtccgccg ctttactact ttgaagaagt tgaagagaag cgagcccgtg aacgtgaaca
    13861 acttgcggcc tggtatcaac cgattccaga accggttaaa gaaccagaac cgatcaaatc
    13921 ttcgctgaaa gcaccttctg ttgcagcagt acctccagta gaagccgctg ccgctgtttc
    13981 tccgctggca tctggcgtga aaaaagcgac actggcgacg ggggctgccg caaccgttgc
    14041 cgcgccagtc ttcagtctgg caaatagcgg tggaccgcgt ccgcaggtca aagaggggat
    14101 tggcccgcag ttgccacgac cgaaacgtat ccgcgtgcca actcgtcgtg aattggcgtc
    14161 ttacggtatt aagctgccct cacagcgtgc ggcggaagaa aaagcccgtg aagcccagcg
    14221 caatcagtac gattctggcg atcagtacaa cgatgatgaa atcgatgcga tgcagcagga
    14281 tgaactggca cgtcagttcg cccagacaca gcagcaacgc tatggcgaac agtatcaaca
    14341 tgatgtgccc gtaaatgcag aagatgcaga tgctgcggca gaggctgaac tggctcgtca
    14401 gtttgcgcaa actcaacaac aacgttattc cggcgaacaa ccggctgggg cgaatccgtt
    14461 ctcgctggat gattttgaat tttcgccaat gaaagcgttg ctggatgatg gtccacacga
    14521 accgctgttt acgccaattg ttgaacctgt acagcagccg caacaaccgg ttgcaccgca
    14581 gcagcagtat cagcagccac aacagccggt tgcgccacag ccgcagtatc agcagccaca
    14641 acagcaggtt gcgccgcagc cacaatatca gcagccgcaa caaccggttg cgccacagca
    14701 gcaatatcag cagccgcaac aaccagttgc gccgcagccg cagtatcaac agccgcaaca
    14761 accggttgca ccgcagccgc agtatcagca gccacaacag ccagttgcgc cacagccgca
    14821 ggatactctg cttcatccgc tgttgatgcg taatggcgac agccgtccgt tgcataaacc
    14881 gacgacgccg ctgccttctc tggatttgct gacaccgccg ccgagcgaag tggagccggt
    14941 agataccttt gcgcttgaac aaatggctcg cctggtggaa gcgcgtctgg ctgatttccg
    15001 tattaaagcc gatgtcgtca attactctcc ggggccggtt atcactcgct ttgaacttaa
    15061 cctggcaccg ggcgtaaaag cggcgcgcat ttctaacttg tcacgggacc ttgctcgttc
    15121 actttcgacg gtggcggtgc gtgtcgttga agttattcct ggcaaaccct atgtaggtct
    15181 ggagttaccg aataaaaaac gacaaaccgt ttatctgcgc gaagtattgg ataacgccaa
    15241 attccgcgat aatccatcgc cattaaccgt ggtgctgggt aaagatatcg ccggtgagcc
    15301 ggtggttgcc gatctggcga aaatgccgca cttgttagtt gcggggacta ccggttccgg
    15361 taaatctgtc ggtgtgaacg cgatgatcct gagcatgctt tataaagcac agccagaaga
    15421 tgtgcgtttc atcatgatcg acccgaaaat gctggagctt tcggtgtatg aaggcattcc
    15481 gcacctgtta acggaagtcg ttactgatat gaaagatgcc gccaacgcgc tgcgctggtg
    15541 tgttaacgag atggagcgtc gttataaact gatgtctgcg ctgggtgtgc gtaatctggc
    15601 gggttataac gaaaaaattg ctgaagccga tcgcatgatg cgtccgattc cagacccgta
    15661 ctggaagccg ggtgacagta tggatgccca gcatccggtg ctgaaaaaag aaccatacat
    15721 tgtggtgttg gtggacgaat ttgccgacct gatgatgaca gtaggtaaaa aagtggaaga
    15781 gctgatagca cgtctggcgc aaaaagcccg tgccgcgggt atccacctcg tactggcaac
    15841 tcagcgtcca tcggttgatg ttattactgg tctgattaaa gcgaatattc cgacccgtat
    15901 cgcctttacc gtatccagta agattgactc acgtaccatt cttgatcagg ctggcgcgga
    15961 atcactgctg ggtatggggg atatgctcta ctctgggccg aactccacgt tgccggtacg
    16021 tgtccatggt gcttttgttc gcgatcagga agttcatgcc gtggtgcagg actggaaagc
    16081 gcgtggtcgc ccacagtatg ttgatggcat cacctccgac agcgaaagcg aaggtggtgc
    16141 gggtggtttc gatggcgctg aagaactgga tccgttgttc gatcaggcgg tgcagtttgt
    16201 cactgaaaaa cgcaaagcgt caatttctgg cgtacaacgt cagttccgca ttggttataa
    16261 ccgtgcagcg cgtattatcg aacagatgga agcgcagggg attgtcagcg aacaggggca
    16321 caacggtaat cgtgaagtgc tgtccccacc gccgtttgac taattaatgc attgccggat
    16381 aaggcgcggt agcgtcgcat ccggcactct atcaactgaa aattcagtat tttcttcttt
    16441 cctcaagctg attattagcc tggaatagag agtagaggga actcccgatc gggagtgacg
    16501 taatttgagg aataatgatg aaaaaaattg ccatcacctg tgcattactc tcaagcttag
    16561 tagcaagcag cgtttgggct gatgccgcaa gcgatctgaa aagccgcctg gataaagtca
    16621 gcagcttcca cgccagcttc acacaaaaag tgactgacgg tagcggcgcg gcggtgcagg
    16681 aaggtcaggg cgatctgtgg gtgaaacgtc caaacttatt caactggcat atgacacaac
    16741 ctgatgaaag cattctggtt tctgacggta aaacactgtg gttctataac ccgttcgttg
    16801 agcaagctac ggcaacctgg ctgaaagatg ccaccggtaa tacgccgttt atgctgattg
    16861 cccgcaacca gtccagcgac tggcagcagt acaatatcaa acagaatggc gatgactttg
    16921 tcctgacgcc gaaagccagc aatggcaatc tgaagcagtt caccattaac gtgggacgtg
    16981 atggcacaat ccatcagttt agcgcggtgg agcaggacga tcagcgcagc agttatcaac
    17041 tgaaatccca gcaaaatggg gctgtggatg cagcgaaatt taccttcacc ccgccgcaag
    17101 gcgtcacggt agatgatcaa cgtaagtaga ggcacctgag tgagcaatct gtcgctcgat
    17161 ttttcggata atacttttca acctctggcc gcgcgtatgc ggccagaaaa tttagcacag
    17221 tatatcggtc agcaacattt gctggctgcg gggaagccgt tgccgcgcgc tatcgaagcc
    17281 gggcatttac attctatgat cctctggggg ccaccgggta ccggcaaaac aaccctcgct
    17341 gaagtgattg cccgctatgc gaacgctgat gtggaacgta tttctgccgt cacctctggt
    17401 gtgaaagaga ttcgcgaggc gatcgagcgc gcccggcaaa accgcaatgc aggtcgccgc
    17461 actattcttt ttgttgacga agttcaccgt ttcaacaaaa gccagcagga tgcatttctg
    17521 ccacatattg aagacggcac catcactttt attggcgcaa ccactgaaaa cccgtcgttt
    17581 gagcttaatt cggcactgct ttcccgtgcc cgtgtttatc tgttgaaatc cctgagtaca
    17641 gaggatattg agcaagtact aactcaggcg atggaagaca aaacccgtgg ctatggtggt
    17701 caggatattg ttctgccaga tgaaacacga cgtgccattg cagaactggt gaatggcgac
    17761 gcgcgccggg cgttaaatac gctggaaatg atggcggata tggccgaagt cgatgatagc
    17821 ggtaagcggg tcctgaagcc tgaattactg accgaaatcg ccggtgaacg tagcgcccgc
    17881 tttgataaca aaggcgatcg cttttacgat ctgatttccg cactgcataa gtcggtacgt
    17941 ggtagcgcac ccgatgcggc gctgtactgg tatgcgcgaa ttattaccgc tggtggcgat
    18001 ccgttatatg tcgcgcgtcg ctgtctggcg attgcgtctg aagacgtcgg taatgccgat
    18061 ccacgggcga tgcaggtggc aattgcggcc tgggattgct ttactcgcgt tggcccggcg
    18121 gaaggtgaac gcgccattgc tcaggcgatt gtttacctgg cctgcgcgcc aaaaagcaac
    18181 gctgtctaca ctgcgtttaa agccgcgctg gccgatgctc gcgaacgccc ggattatgac
    18241 gtgccggttc atttgcgtaa tgcgccgacg aaattaatga aggaaatggg ctacgggcag
    18301 gaatatcgtt acgctcatga tgaagcaaac gcttatgctg ccggtgaggt ttacttcccg
    18361 ccggaaatag cacaaacacg ctattatttc ccgacaaaca ggggccttga aggcaagatt
    18421 ggcgaaaagc tcgcctggct ggctgaacag gatcaaaata gccccataaa acgctaccgt
    18481 taatgttatc gttgcggtaa tgttgttact gtatccctgt ggtcgcaggc tgtggccaca
    18541 tctcccattt aattcgataa gcacaggata agcatgctcg atcccaatct gctgcgtaat
    18601 gagccagacg cagtcgctga aaaactggca cgccggggct ttaagctgga tgtagataag
    18661 ctgggcgctc ttgaagagcg tcgtaaagta ttgcaggtca aaacggaaaa cctgcaagcg
    18721 gagcgtaact cccgatcgaa atccattggc caggcgaaag cgcgcgggga agatatcgag
    18781 cctttacgtc tggaagtgaa caaactgggc gaagagctgg atgcagcaaa agccgagctg
    18841 gatgctttac aggctgaaat tcgcgatatc gcgctgacca tccctaacct gcctgcagat
    18901 gaagtgccgg taggtaaaga cgaaaatgac aacgttgaag tcagccgctg gggtaccccg
    18961 cgtgagtttg actttgaagt tcgtgaccac gtgacgctgg gtgaaatgca ctctggcctc
    19021 gactttgcag ctgcagttaa gctgactggt tcccgctttg tggtaatgaa agggcagatt
    19081 gctcgcatgc accgcgcact gtcgcagttt atgctggatc tgcataccga acagcatggc
    19141 tacagtgaga actatgttcc gtacctggtt aaccaggaca cgctgtacgg tacgggtcag
    19201 ctgccgaaat ttgctggcga tctgttccat actcgtccgc tggaagaaga agcagacacc
    19261 agtaactatg cgctgatccc aactgcagaa gttccgctga ctaacctggt gcgcggtgaa
    19321 atcatcgatg aagatgatct gccaattaag atgaccgccc acaccccatg cttccgttct
    19381 gaagccggtt catatggtcg tgacacccgt ggtctgatcc gtatgcacca gttcgacaaa
    19441 gttgaaatgg tgcagatcgt gcgcccggaa gactcaatgg cggcgctgga agagatgact
    19501 ggtcatgcag aaaaagtcct gcagttgctg ggcctgccgt accgtaaaat catcctttgc
    19561 actggcgaca tgggctttgg cgcttgcaaa acttacgacc tggaagtatg gatcccggca
    19621 cagaacacct accgtgagat ctcttcctgc tccaacgttt gggatttcca ggcacgtcgt
    19681 atgcaggcac gttgccgcag caagtcggac aagaaaaccc gtctggttca taccctgaac
    19741 ggttctggtc tggctgttgg tcgtacgctg gttgcagtaa tggaaaacta tcagcaagct
    19801 gatggtcgta ttgaagtacc agaagttctg cgtccgtata tgaacggact ggaatatatt
    19861 ggctaatacc caatttttct gaatctaaaa agcgcctacg ggcgcttttt ttgtctccct
    19921 ttgataccga acaataatta ctcctcactt acacgtaata ctactttcga gtgaaaatct
    19981 acctatctct ttgattttca aattattcga tgtatacaag cctatatagc gatctgctat
    20041 agaaataatt acacaatacg gtttgttact ggaatcaatc gtgagcaagc ttgagtgagc
    20101 cattatgaaa acgaaaatcc ctgatgcggt attggctgct gaggtgagtc gccgtggttt
    20161 ggtaaaaacg acagcgatcg gcggcctggc aatggccagc agcgcattaa cattaccttt
    20221 tagtcggatt gcgcacgctg tcgatagcgc cattccaaca aaatcaggcg aaaaggttat
    20281 ctggagcgcc tgtacagtta actgtggtag tcgctgcccg ctacgtatgc acgtcgtgga
    20341 cggtgaaatc aaatatgtcg aaacggacaa taccggcgat gacaattacg acggcctgca
    20401 ccaggttcgc gcctgcctgc gtgggcgttc catgcgtcgc cgtgtctaca atccggaccg
    20461 cctgaaatat ccgatgaaac gagtcggggc gcgcggtgaa ggcaaattcg agcgcattag
    20521 ctgggaagaa gcctacgaca tcatcgcgac caatatgcag cgcctgatca aagagtacgg
    20581 caacgaatcc atctatctga actatggcac cggtacgctg ggtggcacca tgacccgctc
    20641 ctggccgccg ggaaataccc tggtcgcgcg gctgatgaac tgctgcggcg gctatctgaa
    20701 ccattacggc gactactcct ccgcgcaaat tgcggaaggt ctgaactata cctacggcgg
    20761 ctgggcagat ggcaacagcc cgtcggatat cgaaaacagt aagctggtag tgctgtttgg
    20821 taataaccct ggcgaaacgc gaatgagtgg cggtggggtg acttactatc ttgaacaggc
    20881 acgccagaaa tctaatgccc gcatgatcat catcgatccg cgctataccg acaccggtgc
    20941 cgggcgcgaa gatgagtgga tccctattcg tccgggaaca gatgccgcac tggttaacgg
    21001 tctggcgtac gtcatgatca ctgaaaacct ggtggatcag gcattcctcg ataaatattg
    21061 cgttggctac gatgagaaaa ccctgccagc cagtgcgccg aaaaatggcc actataaagc
    21121 ttatattctg ggtgaagggc cagatggcgt ggctaaaaca ccggaatggg cctcgcaaat
    21181 caccggtgtt ccggcagaca aaatcatcaa actggctcgt gaaatcggca gtaccaaacc
    21241 ggcgtttatc agccagggat ggggcccgca gcgtcacgct aacggtgaaa tcgcaacccg
    21301 tgctatctcg atgctggcga ttctgaccgg taacgttggt attaatggag ggaacagcgg
    21361 cgcgcgtgaa ggttcataca gtttaccgtt tgtccgtatg ccgaccttgg aaaacccgat
    21421 ccagaccagc atttcgatgt ttatgtggac cgatgccatt gaacgtggcc cggaaatgac
    21481 ggcgctacgt gatggtgtgc gcgggaaaga taagctggat gtgccgatca aaatgatctg
    21541 gaactatgcc ggtaactgcc tgattaacca gcattctgaa atcaaccgta cccatgaaat
    21601 ccttcaggat gataagaagt gcgagctgat tgtggttatc gactgccaca tgacctcatc
    21661 ggcgaaatat gctgacatcc tgctgcctga ctgcaccgct tccgaacaga tggactttgc
    21721 actggatgca tcctgcggga atatgtctta cgtgattttc aacgatcagg tgattaaacc
    21781 gcgctttgaa tgtaagacca tctatgaaat gaccagcgaa ctggcaaaac gtcttggcgt
    21841 tgagcaacag tttactgaag gccgtaccca ggaagagtgg atgcggcatc tgtatgccca
    21901 gtcgcgggaa gcgattcctg aactgccaac gtttgaagag ttccgcaagc aggggatctt
    21961 taaaaagcgc gacccacaag ggcatcacgt tgcttataaa gccttccgtg aagatccgca
    22021 ggcaaatcca ctgaccacgc catcgggtaa aattgagatt tattcgcagg cgctggctga
    22081 cattgccgct acctgggaat tgccagaagg cgatgtgatc gatccactgc cgatctacac
    22141 gccgggcttt gaaagttatc aggatccgct gaacaaacag tatccgctgc agcttacagg
    22201 tttccactat aaatctcgcg ttcactcaac ttacggcaac gttgatgtgc tgaaagcggc
    22261 ttgccgtcag gaaatgtgga tcaacccgct tgatgcccaa aaacgcggta tccacaacgg
    22321 cgataaagtc aggatcttta acgatcgtgg tgaggttcat attgaggcga aagtgacgcc
    22381 acgaatgatg ccgggtgtgg tcgcactggg tgaaggtgcc tggtatgacc cggatgcaaa
    22441 acgtgtcgat aagggtggtt gtattaacgt actgaccact caacgtccgt ctcctctcgc
    22501 taaggggaat ccgtcacata caaaccttgt tcaggttgaa aaggtgtaag gagtaaccga
    22561 tgacaaccca gtatggattt tttattgatt ccagccgttg caccggttgc aaaacctgcg
    22621 agctggcttg taaagactac aaagatttga tgccagaagt cagcttccgc cgcatttatg
    22681 aatatgctgg tggcgactgg caggaagata acggtgtctg gcaccagaac gtgtttgcct
    22741 actatctgtc gatttcgtgc aaccactgcg aagatccggc ttgtactaaa gtctgcccga
    22801 gcggtgcgat gcataaacgt gaagatggtt ttgttgtggt cgatgaagat gtgtgcattg
    22861 gctgccgcta ctgccatatg gcttgcccgt atggcgcacc gcaatataac gaaacgaaag
    22921 gccatatgac caaatgcgat ggttgttatg accgtgttgc ggagggtaaa aagccgatct
    22981 gtgttgaatc ttgtccgctg cgggcgctgg atttcggccc tatcgacgag ctgcgtaaaa
    23041 aacatggcga tctggcggcc gttgcgccgt tgccgcgagc tcactttacc aaaccgaata
    23101 ttgtgatcaa acccaatgcc aatagccgcc cgaccgggga taccactggc tatctggcaa
    23161 acccgaagga ggtgtgagat gggaagtgga tggcatgaat ggccgctgat gatcttcacg
    23221 gtcttcgggc aatgtgtggc gggtggtttt atcgttctgg ctttggcgct gcttaaaggc
    23281 gacctgcgag cagaagccca gcagcgtgtt atcgcctgca tgtttggctt atgggtgctg
    23341 atgggcattg gctttatcgc ctctatgctc catcttggtt caccgatgcg cgcctttaac
    23401 tcgctcaacc gggtaggggc ttcagcactc agtaacgaaa tcgccagcgg ttcgatcttt
    23461 tttgctgtag gcggcatcgg ctggttgctg gcaatgctga aaaagctgtc acctgcattg
    23521 cgtacgctat ggctggtggt gacgatggtt cttggcgtcg tctttgtctg gatgatggtg
    23581 cgtgtgtata acagcattga taccgttccg acctggtaca gcatctggac gccgatgggc
    23641 ttcttcctga cgatgttcat gggcggcccg ttactgggtt acctgttgct gagtctggct
    23701 ggcgtcgatg gctgggcgat gcgtctgctg ccagccattt ctgtactggc actggtggtg
    23761 agtggcgtga tgtcagtgat gcagggcaca gagctggcaa ctattcatag ttctgtgcag
    23821 caggccgcag cgctggtgcc ggactatggt gcgctgatgt cctggcgaat cgtgcttttg
    23881 gccgttgccc tgtgcttgtg gattgcacca cagctaaaag gttatcagcc tgcggttccg
    23941 ctactatccg tctcattcat tctgcttctg gcaggggaat taatcggtcg cggcgtattc
    24001 tatggtttgc atatgaccgt ggggatggcc gtcgcaagct aatcagtaac aaccggggtt
    24061 tcggccccgg ttctctttta tttctgcttc gttaacgtgt cataactggt catcaaatta
    24121 cgataatcag gaatgtggtt agagaacaac gtcgccagtc cttcaatatc attacgccag
    24181 tcacggtgca gctcgcaagc cacgccgaac catgtcatca attgcgcacc tgcttgcgac
    24241 atgcgatccc atgccgaatg ccgggtaatt tcattaaagg tccctgacgc gtcagtcaca
    24301 acaaagacat caaacccttc ttcaatggct gaaagcgccg ggaatgccac gcaaacttcg
    24361 gttaccacac cggcaataat taactgtttt ttacctgtcg ctttgactgc ttttacaaaa
    24421 tcttcgttat cccaggcgtt aatattgcca gggcgagcaa tgtaaggtgc atcggggaat
    24481 tgtgctttca gctctggaac taatgggccg ttaggaccgg tttcaaaact ggtggtaaga
    24541 atagttggta agttgaaata cttagccagg tcacccagcg ccagcacgtt atttttaaac
    24601 ttatcgggtt cgatatcccg tacaagggaa agtaaaccag cctggtgatc aacaagcaaa
    24661 acggcagcat catttttatc aagacgaaca tacggtttgg tcatcctctt ctcctttcga
    24721 gagtcagtgg tacgtctgaa atcggtcaga cgcgatgcat tgctctgaaa gcatagacgg
    24781 gaaatatgag tttgctgtga ccatgaaatt tttcgactga acgaagcgta tgaaatttgt
    24841 gttagatcaa taaaaacaat cagatgagat taatcatctc ttcagcatta gcgttatgaa
    24901 tgagtaatat aaatcgataa taccgccgct gcgatatgcg cgtattttca cccattgaca
    24961 atgttttttg cggtggcatg atgcgcatga aatttgaact tcctcacggt tttaattcat
    25021 gtccacgtat acccggcctg tcatgctttt gctgtctggc ctgcttttgt tgactctggc
    25081 gatcgcggtg ttaaatacac tcgtgccgct ttggctcgcc caggaacaca tgtccacatg
    25141 gcaggtaggc gttgtcagct catcctattt taccggcaac cttgtcggta cattgctgac
    25201 agggtatgtc attaaacgca ttggctttaa ccgcagctat tatctggcct ccttcatttt
    25261 tgccgctggc tgtgccggcc ttggcctgat gattggtttc tggagctggt tggcgtggcg
    25321 ttttgtcgcg ggcgtcggct gtgccatgat ttgggtagtc gttgagagcg cgctgatgtg
    25381 cagtgggacg tcacgtaacc gtgggcgttt gcttgctgcg tatatgatgg tttattacgt
    25441 gggaacattt ttaggccagt tactggtcag caaagtctca accgagctga tgagcgtatt
    25501 gccgtgggtt acaggtttga cgttggcagg gatcttaccg ctgttgttta cgcgtgtcct
    25561 gaatcagcag gctgaaaacc atgattcgac gtcaattacg tcaatgctaa aactccgtca
    25621 ggcgcggctt ggcgtgaatg gctgcattat ctcaggaatc gtgctgggat ctctatatgg
    25681 cctgatgccg ctgtacctca atcacaaagg ggtgagcaat gccagcattg gtttctggat
    25741 ggcggtactg gtcagtgcgg gtatcctcgg acaatggccg attggacgtc tggcggataa
    25801 gtttggtcga ctgctggtgt tgcgtgttca ggtctttgtc gtcattctcg gcagtatcgc
    25861 gatgcttagc caggcggcga tggccccagc gttattcatc ctcggtgccg ctggctttac
    25921 gctatatccg gtggcgatgg cctgggcttg tgagaaagtt gaacatcatc aactggtggc
    25981 gatgaaccag gccttactgt tgagctatac cgtgggaagt ctgcttggcc cgtcatttac
    26041 cgctatgcta atgcagaatt tctccgataa tttattgttt atcatgatcg ccagcgtatc
    26101 gtttatctat ttgctgatgc tgcttcgcaa cgccggtcat acgccgaaac ccgttgctca
    26161 cgtgtaaatg aattcaagca aagtgtgaac ttactgtttc acactctgct tttttgtttc
    26221 ttctatctga cttgcttttt tccaaatttt attcgtttaa aaataaaatg tgcagcaggt
    26281 tataattttg catttcgcta tttccgcact tcttatttgc cgcgcataat ccctcgtttt
    26341 acagatgccc ctttaatttt ggcgaaggat ttgtctatgg ctgggaatgt tcaggaaaaa
    26401 cagctgcgat ggtacaacat tgcgctgatg tcttttatca ctgtctgggg ttttggcaac
    26461 gttgttaata actatgccaa ccaggggctg gtggttgttt tttcatgggt gtttatcttt
    26521 gcgctctatt tcacacctta tgcactaatt gttggtcagt taggctcgac cttcaaagat
    26581 gggaagggcg gggtcagtac ctggattaaa cacacgatgg gacccggatt ggcttacctc
    26641 gccgcgtgga cctactgggt ggtgcatatt ccctatctgg cacaaaaacc ccaggcaata
    26701 ctgattgcgc tcggttgggc gatgaaaggc gacggttcgc taatcaaaga atattcagtc
    26761 gtagcgttac aggggttaac gctggtgctg tttatcttct ttatgtgggt tgcttcacgc
    26821 ggtatgaaat cgctgaaaat cgtcggttct gtggcaggga ttgcgatgtt tgttatgtcg
    26881 ctcctgtatg tggcgatggc ggtaaccgcg cctgcgatta ctgaagtgca tattgcgacc
    26941 acaaacatta cctgggaaac gttcattcct catatcgact ttacctacat taccactatt
    27001 tcaatgctgg ttttcgcggt tggcggagct gagaagattt ctccttacgt taatcaaacg
    27061 cgcaacccag gaaaagaatt tccaaaaggg atgttatgcc tggcggtgat ggttgcggtt
    27121 tgtgccattc tgggctcact ggcgatgggg atgatgtttg actcgcgtaa tatcccggat
    27181 gacttaatga ctaacggtca gtattacgcc tttcagaagc tgggcgagta ttacaacatg
    27241 ggtaatactt taatggtgat ttacgccatt gcgaataccc tgggacaagt agctgcgctg
    27301 gtattctcga ttgatgcccc gcttaaagtg ctattaggcg atgctgacag caaatatatt
    27361 ccagccagtt tatgtcgtac caacgcttct ggtacgcccg ttaatggcta ttttctgacc
    27421 ctggtgctgg tggcgatcct gattatgctg ccgactctcg gcatcggtga tatgaacaat
    27481 ctctataaat ggctgttgaa tcttaactcg gtagtgatgc cgctgcgtta tttatgggta
    27541 tttgttgcat ttattgcagt cgttcgcctg gcgcagaaat atacaccaga gtatgtcttt
    27601 attcgtaata agcctctggc aatgaccgtc gggatttggt gttttgcctt tactgccttt
    27661 gcctgtttga cggggatctt cccgaaaatg gaagccttca ctgcagagtg gaccttccag
    27721 ttggcgctga atgttgcaac gccgtttgtg ctggtaggat taggactgat attcccgctg
    27781 ctggcgcgta aagcgaatag taaataatta ttgtggtcgg tgacgctgac tctggcagat
    27841 ctgacccgtt tttacataat cgagaaatgc gcgcaagccg caggacatat actggcggtt
    27901 tgggtaatac atttggaagc tgtgctgtaa gggcgttaga aatagagctc tgaattattt
    27961 atgcagcgcg gaataaaaaa gcggcaactc aataaagttg ccgctttacg gggaaattag
    28021 aacattacct tatgaccgta ctgctcaaga atacctttca cgcgttccat ggtttctttc
    28081 ttcggtggtt taacaccatc gagtttgtat tcttcaccca tcgccaccca tttgtgtttg
    28141 cccagttcgt ggtaggggag aagttcgatt ttctcaacgt tacccatatc acgggtaaat
    28201 tcaccgaggc gatgcgctga atcgtcatcg tcagaccagc ctgggacaac aacgtagcgg
    28261 atccacacct tcacattttt attcgccaga tatttggcga actccagcgt gcggtggttg
    28321 gaaactccaa ccagattttg gtggatctcg tcgttcatct gtttgagatc gagcattacc
    28381 aggtcggtta cttccagcag ttcatcaatc accggatcgt aacgacgaac aaaaccgttg
    28441 gtgtccagac aggtatgaat gccttctttt ttgcaggcgc ggaaccagtc acgaacaaac
    28501 tcagcttgca ggattgcttc accgccggat gcggtaacgc cgccgccgga agcgttcata
    28561 aagtggcgat aggtcaccac ttccttcatc aaatcttcaa cggtaacttc tttaccgcca
    28621 tgcgtgtccc aggtgtcgcg gttatgacaa tacaggcagc gcatcaggca gccctggaaa
    28681 aaggtgataa agcgaatacc tgggccgtct acggttccac aggattcaaa ggagtgaatg
    28741 cgaccaataa ctgacattgc ggtgtttctc cagatgtggc ccatctgagg ccgtgttggt
    28801 gcgcagctcg aaggctacgt cgagtctgtt ttggcagtca ccttaaagta tagatagctg
    28861 acaaaaaagg ctcacgtgct aaaaaaggcc ccactttcgt ggagccttta ttgtacgctt
    28921 tttactgtac gatttcagtc aaatctaatt acatagattg agtgaaggta cgagtaataa
    28981 cgtcctgctg ctgttcttta gtcagcgagt tgaaacgtac tgcgtagcca gatacacgga
    29041 tggtcagctg cggatatttt tccggatttt ccatcgcgtc gagcagcatt tcacggttca
    29101 tcacgttaac gttcaggtgc tgaccacctt cgatggatgc ttcgtggtgg aagtaaccat
    29161 ccatcagacc agccaggttg gtcttacgaa cttcgtcgtc tttacccagt gcgttcggaa
    29221 cgatagagaa ggtgtaggag ataccatctt tagcgtaagc aaacggcagt ttagcaacgg
    29281 aagtcagaga cgctacagca cctttctggt cacgaccgtg catcgggtta gcacccggtc
    29341 cgaacggcgc gccagcacga cgaccgtctg gggtgttacc cgttttctta ccatacacaa
    29401 cgttagaagt gatggtcaga acagactgag tcgggatagc gtcacggtag gtgtgcagtt
    29461 tctgaatttt cttcatgaaa cgttctacca ggtcaacagc caggtcatct acacgcggat
    29521 cgttgttacc aaactgcggg tattcgcctt cgatttcgaa gtcgatagcc agaccgtctt
    29581 cgtcacgaat cggtttaact ttcgcatatt tgattgcaga cagggagtca gcagcaacgg
    29641 acagaccagc gataccacac gccatggtgc ggataacgtc acggtcgtgc agcgccatca
    29701 gagaggcttc gtagctgtac ttgtcgtgca tgtagtgaat gatgttcagt gcagtgatgt
    29761 actgtttagc cagccagtcc atgaagtgat ccatgcgctc catcacttca tcgtagttca
    29821 ggagatcgcc tttgatcggt tcagacttcg gaccaacctg cattttcagt ttttcgtcaa
    29881 cgccgccgtt gattgcgtac agcatggttt tcgccaggtt tgcacgcgca ccgaagaact
    29941 gcatttgttt accaacgatc atcgggctta cgcagcaagc gatagcgtag tcatcgttgt
    30001 tgaagtccgg acgcatcagg tcatcgttct catactgcag agaagaggtg tcgatggaca
    30061 ctttagcggc gaatttcttg aagttcagcg gcagtttttc agaccacaga atggtcatgt
    30121 tcggttccgg agacggaccc atggtgtaca gggtgttcag gaaacggaag ctgtttttgg
    30181 taaccagggt acgaccgtcg aggcccatac caccgataga ttcggttgcc cagatcgggt
    30241 cgccagagaa cagttcatcg tactccggag tacgcaggaa gcgaaccata cgcagtttca
    30301 tgaccaggtg gtcaaccatt tcctgcgctt cttgttcggt gatcttgcca gctttcaggt
    30361 cacgttcgat gtacacatcc aggaaggtgg aggtacgacc gaaggacatt gcagcaccgt
    30421 tctgagactt aacagcagcc aggtagccga agtaagtcca ctggatagct tcctgagcgt
    30481 tggtagccgg accagagatg tcgtagccgt atttcgcagc catttctttc atctgaccca
    30541 gagcgcggtg ctgttcagcg atttcttcgc gcagacggat agtctgttcc aggtttacgc
    30601 cgttttccag atcagcctgc agagaggtga actgagcgta tttgtctttc atcaggtagt
    30661 cgataccgta cagcgcaacg cgacggtagt caccgatgat acggccacgg ccataagcat
    30721 ctggcagacc ggtcagaaca ccggatttac ggcaacgcag gatgtccgga gtgtaaacgt
    30781 cgaacacgcc ctggttgtga gttttacggt attcagtgaa gatttttttg atcatcgggt
    30841 ccagttcgcg gttgtacgct ttgcaggaac cttcgatcat tttgatacca ccgaacggga
    30901 taagagcacg tttcagcgga gcttcagtct gcagaccaac aactttttcc aacgctttgt
    30961 tgatgtagcc agcgtcgtga gaggtgatgg tggaagcaac agcggtgtcg aagtcaactg
    31021 gcgcgtgagt gcggttttcc agtttaacgc cttccattac tttgtcccac agggtggtgg
    31081 tcgcttcagt agcgccagcc aggaaggact cgtcaccctc gtacggagtg tagtttttct
    31141 gaatgaagtc acggacgttt acttcattct gccagtcacc tttggtaaaa ccttcccagg
    31201 ctgtggctaa cttttcatta agctcggaca tgtaacacct accttcttaa gtggattttt
    31261 tatttactgc gtacttcgac aaccattaat ggtgatcgtt ttcacgcagg taaatgaccc
    31321 agtatgtcaa cccaaccaac aaaccaccgc cgataatatt accgatcgta accggaatca
    31381 ggttatcagt gatgaagttc atcacggtca ggtgagaaaa attttccggt gcagaaccga
    31441 cagcggtcca gaattccgga gatgcgaagt cgcggattac aatacccatc gggatcataa
    31501 acatgtttgc gatactgtgc tcaaaaccgc tggcaacaaa catcgcgacc ggcagcacca
    31561 tgataaacgc tttgtccatc aggctgcggc cagaataact catccatact gccagacata
    31621 ccatcaggtt tgccaggata ccaaggcaga cggcctcaat aaaagtatgg tgcactttgt
    31681 ggtcggcggt ttgtaggacg tttagtcccc attgaccatt tgcggtcata tactcgccgg
    31741 aaagccacat taaaagtaca aacagcagtg cgccgaccag gttgccaaaa tagacattta
    31801 gccagttttt agccaactga ccccaggtga tgcgcccact cgccttagca acaacaatca
    31861 acactgtgga agtaaagaga tcggctccgc agacaacaca aagaatcagt cccagagaga
    31921 agcaaatgcc gccaaccagt tttgccatgc cgaagggcat tgtgcctgtg ccagtggttg
    31981 ctgtgatata gaagacgaat gcgattgaga tgaaaacacc ggcggtaatc gccagataga
    32041 aagtcttaag cggatgtttc gttgctttat agacacccgc ctcttcggcc actttggcca
    32101 ttgcagcagg aagtaaaaga tcaaaagggt tgtcagcttt cacactaact ctctctttat
    32161 taagtcggcg acgagatact aacaaagcat tatagatgag aaattgatat agatcatatc
    32221 tcgcctggct tataggcccg taactcgcat ggtttttatg caaatacgga gtaaatattt
    32281 gattatccaa ataaaaataa attttaaaaa ttaacaaatg agttgaattt tttccgcatc
    32341 ctccgctaaa acagttaatt aaaagggagc atcaggcgaa taaagtaaca atatcgatcg
    32401 tatttattaa atacaaatta ccgatattta accttataat tacaattatt ttattaatgc
    32461 aaatatatgt aaagcggggc attaaaaaaa cgccccgtaa tataactcag acgaatcatt
    32521 aagcctacat tacgtaggct atttgatttt attttgccca gaatgctgct ttggcgcgct
    32581 gcagcttttc gtaggccttc aacagcgact gatgtgcagc aaacgcgtgc agatcgctat
    32641 ctactggttg caagccataa aacgccgctt cgccgctcat tgccgcactg gcggcttcca
    32701 cggcatctgc gccgtacatg cgaacaaacg cattcagata ttgcagcggc tggcgatctt
    32761 cttcctgcgc cagtaataac aacgtttgca ggcagcgata atagttggcg cgttccgggc
    32821 tgaataccga tgagttaaac tccatcgtcc attcggtcca gaccagagcc tgttccagat
    32881 cgccaccagc cagcgccagc atggctttta attcaccaat acgcagggtg taccaaccgt
    32941 tatccgaccc ggtcgccaga cccaacagct cacgcacgcg ggtaaagtca tcaaaacctt
    33001 cttcatccag ttgctcgatg aggttcaggt aatcttcttt ttcccactcg ctgcctggta
    33061 gcgaaagaat cgtttcacgt aaatggctgc ccatactgtt attcgcgagc cacagatctt
    33121 cagccggata aatatcggac atgccaggca cgataatacg gcaagcataa acgcccagat
    33181 gctcgtaatc ggcaatataa acttctttat cttctttgtt gaagatagcc atcagcgtag
    33241 caaactcttc ttccgtggtg ccggagaaat tccagtccac aaacggataa tcggcatcct
    33301 gcttgaacag gtcccaggag attaaaccgc tggaatcgat aaagtgcgtt tcgaggttgg
    33361 tatgttcagc gacttcttca tcatcgaagg ttggcggagt aaacacatcc aaatctttca
    33421 ggccacgacc ttgcagcagc tcggtcacgg tacgttccag tgctacgcca aaatcaggat
    33481 gcgcaccgaa agaggcaaag caggtgccgt tagcaggatt aaacagtacc acgcaaatca
    33541 ccggatactg gccgccaagg gaaccatcat atgcgaagat cgggaaacct tctgcttcca
    33601 gtgtttcgat cgcttcaacc accgctgggt aacgcgccag cacgtctgcc gggatttccg
    33661 gcaagctgat gctttcagca ataatgcggt ttttcacgta gcgttcgaaa acttcggaca
    33721 acccctgaac gcgtgcttcg ttacgggtat tgccagcaga cataccgtta gaaacgtaca
    33781 ggttaccaat gatattcatc ggaatataaa cggtctgatt atcagactga cgtgtaaacg
    33841 gcagaccgca aataccacga tcttcgttac cggattgtag gtcaatcagc atactgccgg
    33901 tcaattcatt ctccggatcg taaaatgcgc gcagacgttc atcgagcagc ccttctggca
    33961 catcgtcatt ttcggtcagt gggaaccatt tttcgttggg ataatgcaca aatggaccgt
    34021 tggcgatggt ttcgcccagc cagaaatcag caaaaaagta gttggttgag agacgctcga
    34081 aatattcacc gagtgcagaa gccagcgccg ctttcttggt tgcgccttta ccgttggtaa
    34141 aacacagtgc gcactctttg tcgcgaatat gtacagacca gacgttaggc acgggattca
    34201 gccaggaggc ctcttcaatc tgaaagccga ggtctgaaag tttttgctgg aagcgagcga
    34261 tggaatcttc cagtgcggca tctttgccgg ggataaatgt ttgcgtcatg aaaatcactt
    34321 tagtcgtacg gaaagcgcgc aataatacgg gttttatctc aaaggcgcta tcaccgccgc
    34381 catgccgatg aactgttgac tatgctttta gcggataaac cacgttaaga gcataaaaat
    34441 gaaggcgttc gatctccacc gtatggcatt tgataaagtg ccttttgatt tccttggcga
    34501 agttgcacta cgtagtcttt atacctttgt actggtcttt ttgttcctca aaatgaccgg
    34561 aagacgcggt gtgcggcaga tgtcgctgtt tgaagtttta atcattctga cgctgggatc
    34621 agcggcggga gatgtggcgt tttatgatga tgtgccgatg gtcccggtac ttatcgtctt
    34681 tattactctg gcgttgttat accgcctggt aatgtggttg atggcgcaca gtgaaaaact
    34741 ggaagatctt ctggaaggca agccagttgt cattattgaa gatggcgagc tggcctggtc
    34801 gaaactcaat aactccaaca tgacggaatt tgagttcttt atggagctac gattgcgtgg
    34861 cgtggagcag ctggggcagg tacgtctggc gattctcgaa accaacgggc aaatcagtgt
    34921 ctatttcttt gaagatgaca aggtgaaatc gggtttactt attttaccca gtgattgtac
    34981 gcagcgttac aaagtggtgc cggagtcggc ggactatgcc tgtattcgtt gtagtgaaat
    35041 cattcatatg aaagcggggg aaaaacaatt atgtccgcgc tgtgcaaatc cagaatggac
    35101 gaaggcaagt cgggcaaaac gggtgacctg acagtaaaaa catcggcttt ttgctaataa
    35161 tccgagagat tcttttgtgt gatgcaagcc acatttttgc cctcaacggt tttactcatt
    35221 gcgatgtgtg tcactgaatg ataaaaccga tagccacagg aataatgtat tacctgtggt
    35281 cgcaatcgat tgaccgcggg ttaatagcaa cgcaacgtgg tgaggggaaa tggctcaaat
    35341 cttcaatttt agttctggtc cggcaatgct accggtagag gtgcttaaac aggctcaaca
    35401 ggaactgcgc gactggaacg gtcttggtac gtcggtgatg gaagtgagtc accgtggcaa
    35461 agagttcatt caggttgcag aggaagccga gaaggatttt cgcgatcttc ttaatgtccc
    35521 ctccaactac aaggtattat tctgccatgg cggtggtcgc ggtcagtttg ctgcggtacc
    35581 gctgaatatt ctcggtgata aaaccaccgc agattatgtt gatgccggtt actgggcggc
    35641 aagtgccatt aaagaagcga aaaaatactg cacgcctaat gtctttgacg ccaaagtgac
    35701 tgttgatggt ctgcgcgcgg ttaagccaat gcgtgaatgg caactctctg ataatgctgc
    35761 ttatatgcat tactgcccga atgaaaccat cgacggtatc gccatcgacg aaacgccaga
    35821 cttcggcaaa gatgtggtgg tcgccgccga cttctcttca accattcttt cccgtccgat
    35881 tgacgtcagc cgttatggtg taatttacgc tggcgcgcag aaaaatatcg gcccggcagg
    35941 cctgacaatc gtcatcgtcc gtgaagattt gctgggcaaa gcgaatgtcg cgtgtccgtc
    36001 gattctggat tattccatcc tcaacgataa cggctccatg tttaacacgc cgccgacatt
    36061 tgcctggtat ctatctggtc tggtctttaa atggctgaaa gcgaacggcg gtgtagctga
    36121 aatggataaa atcaatcagc aaaaagcaga actgctatat ggggtgattg ataacagcga
    36181 tttctaccgc aatgacgtgg cgaaagctaa ccgttcgcgg atgaacgtgc cgttccagtt
    36241 ggcggacagt gcgcttgaca aattgttcct tgaagagtct tttgctgcag gccttcatgc
    36301 actgaaaggt caccgtgtgg tcggcggaat gcgcgcgtct atttataatg ccatgccgct
    36361 ggaaggcgtt aaagcgctga cagacttcat ggttgagttc gaacgccgtc acggttaatg
    36421 ccgaaatttt gcttaatccc cacagccagc ctgtggggtt tttatttctg ttgtagagag
    36481 ttgagttcat ggaatccctg acgttacaac ccatcgctcg tgtcgatggc actattaatc
    36541 tgcccggttc caagagcgtt tctaaccgcg ctttattgct ggcggcatta gcacacggca
    36601 aaacagtatt aaccaatctg ctggatagcg atgacgtgcg ccatatgctg aatgcattaa
    36661 cagcgttagg ggtaagctat acgctttcag ccgatcgtac acgttgcgaa attatcggta
    36721 acggcggtcc attacacgca gaaggtgccc tggagttgtt cctcggtaac gccggaacgg
    36781 caatgcgtcc gctggcggca gctctttgtc tgggtagcaa tgatattgtg ctgaccggtg
    36841 agccgcgtat gaaagagcgc ccgattggtc atctggtgga tgcgctgcgc ctgggcgggg
    36901 cgaagatcac ttacctggaa caagaaaatt atccgccgtt gcgtttacag ggcggcttta
    36961 ctggcggcaa cgttgacgtt gatggctccg tttccagcca attcctcacc gcactgttaa
    37021 tgactgcgcc tcttgcgccg gaagatacgg tgattcgtat taaaggcgat ctggtttcta
    37081 aaccttatat cgacatcaca ctcaatctga tgaagacgtt tggtgttgaa attgaaaatc
    37141 agcactatca acaatttgtc gtaaaaggcg ggcagtctta tcagtctccg ggtacttatt
    37201 tggtcgaagg cgatgcatct tcggcttctt actttctggc agcagcagca atcaaaggcg
    37261 gcactgtaaa agtgaccggt attggacgta acagtatgca gggtgatatt cgctttgctg
    37321 atgtgctgga aaaaatgggc gcgaccattt gctggggcga tgattatatt tcctgcacgc
    37381 gtggtgaact gaacgctatt gatatggata tgaaccatat tcctgatgcg gcgatgacca
    37441 ttgctacggc ggcgttattt gcaaaaggca ccaccacgct gcgcaatatc tataactggc
    37501 gtgttaaaga aaccgatcgc ctgtttgcga tggcaacaga actgcgtaaa gtcggtgcgg
    37561 aagtagaaga ggggcacgat tacattcgta tcactccacc ggaaaaactg aattttgccg
    37621 agatcgcgac atacaatgat caccggatgg cgatgtgttt ctcgctggtg gcgttgtcag
    37681 atacaccagt gacgattctt gatcccaaat gtacggccaa aacatttccg gattatttcg
    37741 agcagctggc gcggattagc caggcagcct gaatgaataa cgtccaataa atagccaaaa
    37801 ctttctttat caaaacgtcg gcacattgcc ggcgtttttt ttcggacctt gtgagtcatt
    37861 ttgattaatg gtagcgtcgc ttgtcaatgt aagttgttga tacataatgt ttatatatga
    37921 ttaatcaacg gatgattcac atgaagaata ctaaattact gctggcgatt gtgacctctg
    37981 cagcattact gacggggtgt caaaataccc acggtattga taccaatatg gctatcagct
    38041 ccggtttaaa tgcctataaa gcagcaacgt taagcgatgc cgatgcaaaa gcgattgcca
    38101 atcagggctg tgccgaaatg gacagcggca atcaagtcgc aagtaaatcc agcaagtacg
    38161 gtaaacgtct ggcaaaaatc gccaaagcat tgggtaacaa tattaatggc acgccggtca
    38221 actataaggt ttatatgacc agcgacgtca acgcatgggc gatggcgaac ggctgtgttc
    38281 gtgtttacag tggcctgatg gacatgatga acgataacga aattgaaggc gttctgggcc
    38341 atgaactggg ccacgtcgcg ttgggtcact cgctggctga aatgaaagct tcttatgcga
    38401 tcgttgccgc acgcgatgcc atttcagcta ccagcggtgt ggcttcccag ctttcccgct
    38461 cacaattggg tgatatcgca gaaggcgcta tcaatgcgaa atactcccgc gataaagagt
    38521 ccgaagcaga tgatttctcc tttgatctgt tgaagaaacg tggcatcagc acccaggggc
    38581 tggttggcag ctttgaaaaa ctggctagcc tggatggcgg tcgcacccag tccatgtttg
    38641 actctcaccc accatcaaca gagcgtgcgc aacacatccg tgatcgtatc gcctctggta
    38701 agtaaatcat tgtcatcttt cgggctggtc ttctgccagc ccgctataat tgcgcaataa
    38761 atccccatct gaatacagac aaaactggtt tttgcacaca acgttaacga tttgtggcgt
    38821 cggcgcgtat aatgcgcgcg gttatgttaa cggtacgcct gttttaagga gataaagatg
    38881 acggcaattg ccccggttat taccattgat ggcccaagcg gtgcagggaa aggcaccttg
    38941 tgtaaggcta tggcggaagc gttgcaatgg catctgctgg attcgggtgc aatttatcgc
    39001 gtactggcac tagcggcatt acatcaccat gttgatgttg catcggaaga tgcgctggtg
    39061 ccgctggcat cccatctgga tgtacgtttt gtgtcaacca atggcaatct ggaagtgatc
    39121 ctcgaagggg aagatgtcag cggcgaaatt cgtactcagg aagtggcgaa tgcagcttca
    39181 caagtcgcgg cattcccacg cgttcgtgaa gcattattgc gtcgccaacg cgcgtttcgc
    39241 gaattaccag gcctgattgc cgatggccgc gacatgggaa cggtggtatt ccctgatgcg
    39301 ccagtgaaaa ttttccttga cgcctcctcg gaagaacgtg cgcatcgccg catgctacag
    39361 ttgcaggaga agggctttag tgttaacttt gagcgccttt tggccgagat caaagaacgc
    39421 gacgaccgcg atcgtaaccg agcggtagcg ccactggttc cggcagccga tgctttagtg
    39481 ttggattcca ccaccttaag cattgagcaa gtgattgaaa aagcgctaca atacgcgcgc
    39541 cagaaattgg ctctcgcata agcgaccgaa tttgcagtac ccccgttgca atggaatgac
    39601 agcgggtatg ttaaacaacc ccatccggca tggagccagg tggacgttaa atataaacct
    39661 gaagattaaa catgactgaa tcttttgctc aactctttga agagtcctta aaagaaatcg
    39721 aaacccgccc gggttctatc gttcgtggcg ttgttgttgc tatcgacaaa gacgtagtac
    39781 tggttgacgc tggtctgaaa tctgagtccg ccatcccggc tgagcagttc aaaaacgccc
    39841 agggcgagct ggaaatccag gtaggtgacg aagttgacgt tgctctggac gcagtagaag
    39901 acggcttcgg tgaaactctg ctgtcccgtg agaaagctaa acgtcacgaa gcctggatca
    39961 cgctggaaaa agcttacgaa gatgctgaaa ctgttaccgg tgttatcaac ggcaaagtta
    40021 agggcggctt cactgttgag ctgaacggta ttcgtgcgtt cctgccaggt tctctggtag
    40081 acgttcgtcc ggtgcgtgac actctgcacc tggaaggcaa agagcttgaa ttcaaagtaa
    40141 tcaagctgga tcagaagcgc aacaacgttg ttgtttctcg tcgtgccgtt atcgaatccg
    40201 aaaacagcgc agagcgcgat cagctgctgg aaaacctgca ggaaggcatg gaagttaaag
    40261 gtatcgttaa gaacctcact gactacggtg cattcgttga tctgggcggc gttgacggcc
    40321 tgctgcacat cactgacatg gcctggaaac gcgttaagca tccgagcgaa atcgtcaacg
    40381 tgggcgacga aatcactgtt aaagtgctga agttcgaccg cgaacgtacc cgtgtatccc
    40441 tgggcctgaa acagctgggc gaagatccgt gggtagctat cgctaaacgt tatccggaag
    40501 gtaccaaact gactggtcgc gtgaccaacc tgaccgacta cggctgcttc gttgaaatcg
    40561 aagaaggcgt tgaaggcctg gtacacgttt ccgaaatgga ttggaccaac aaaaacatcc
    40621 acccgtccaa agttgttaac gttggcgatg tagtggaagt tatggttctg gatatcgacg
    40681 aagaacgtcg tcgtatctcc ctgggtctga aacagtgcaa agctaacccg tggcagcagt
    40741 tcgcggaaac ccacaacaag ggcgaccgtg ttgaaggtaa aatcaagtct atcactgact
    40801 tcggtatctt catcggcctg gacggcggca tcgacggcct ggttcacctg tctgacatct
    40861 cctggaacgt tgcaggcgaa gaagcagttc gtgaatacaa aaaaggcgac gaaatcgctg
    40921 cagttgttct gcaggttgac gcagaacgtg aacgtatctc cctgggcgtt aaacagctcg
    40981 cagaagatcc gttcaacaac tgggttgctc tgaacaagaa aggcgctatc gtaaccggta
    41041 aagtaactgc agttgacgct aaaggcgcaa ccgtagaact ggctgacggc gttgaaggtt
    41101 acctgcgtgc ttctgaagca tcccgtgacc gcgttgaaga cgctaccctg gttctgagcg
    41161 ttggcgacga agttgaagct aaattcaccg gcgttgatcg taaaaaccgt gcaatcagcc
    41221 tgtctgttcg tgcgaaagac gaagctgacg agaaagatgc aatcgcaact gttaacaaac
    41281 aggaagatgc aaacttctcc aacaacgcaa tggctgaagc tttcaaagca gctaaaggcg
    41341 agtaattctc tgactcttcg ggatttttat tctgaagttt gttgagttta cttgacagat
    41401 tgcaggtttc gtcctgtaat caagcactaa gggcggctac ggccgccctt aatcaatgca
    41461 gcaacagcag ccgcttaatt tgcctttaag gaaccggagg aatcatgacc aagtcagaat
    41521 tgatagaaag acttgccacc cagcaatcgc acattcccgc caagacggtt gaagatgcag
    41581 taaaagagat gctggagcat atggcctcga ctcttgcgca gggcgagcgt attgaaatcc
    41641 gcggtttcgg cagtttctct ttgcactacc gcgcaccacg taccggacgt aatccgaaga
    41701 ctggcgataa agtagaactg gaaggaaaat acgttcctca ctttaaacct ggtaaagaac
    41761 tgcgcgatcg cgccaatatt tacggttaag tttttactca aacttgaacg agagaaaagc
    41821 acctgtcggg tgcttttttc atttatctaa tctggaactg gaagctgcct cgcagagttt
    41881 tgagcagttt tcaccctttc gttaaattct tctgaatgtg cctcggggaa cgcaaaattc
    41941 ccaccagaca accgctcaac aaagttgcac actttccata aacagggagg ggtgcatgaa
    42001 aataacgaca gtcggcgtat gcataatttg cggaattttt ccgttgctga ttttgcccca
    42061 attgcctggg acattaaccc ttgcgtttct gactctcttc gcctgcgtac tggcatttat
    42121 ccctgttaaa accgtccgtt atatcgcgct gacgttgctg tttttcgttt ggggcatatt
    42181 atcagcaaag caaattttgt tggcaggaga aaccttaact ggcgcgacgc aggatgcaat
    42241 tgttgagatc actgctactg acggcatgac cactcattac ggtcaaatta cttatctaca
    42301 aggtcaacgt atattccctg cgccaggcct tgtgctgtat ggcgaatatc ttccgcaagc
    42361 ggtttgtgcc ggacaactgt ggtcaatgaa actcaaagtt cgtgccgttc atggtcaact
    42421 taatgatggc ggctttgata gccagcgtta tgccattgcc cagcatcagc cgctcaccgg
    42481 ccgctttctg caggcaagtg ttattgaacc gaattgtagc ctgcgtgcac agtatctggc
    42541 gtcactacaa acaacgctgc aaccctatcc gtggaatgcg gttattcttg gtttaggtat
    42601 gggggaacgg ttatccgtcc ctaaagaaat caaaaatatc atgcgtgata ctggaacggc
    42661 gcatttaatg gcgatatcgg gattgcacat cgcttttgcg gcgttgctgg ctgccggact
    42721 cattcgcagt ggacaaattt ttctgcctgg gcgctggatc cactggcaaa tgccattaat
    42781 tggcggaatc tgctgtgctg ctttttatgc ctggctgacg ggaatgcaac ctcctgcatt
    42841 gcgtaccgtt gtggcgcttg ctacgtgggg tatgcttaag ttaagtgggc gacagtggag
    42901 tggctgggat gtatggatat gttgtctggc ggcaattttg ctgatggatc ctgttgtcat
    42961 tctctcgcaa agtttatggc tctctgccgc tgcggtcgcg gcattgatat tttggtatca
    43021 gtggtttccc tgtcctgagt ggcaactgcc gccggtattg cgtgcagttg tttccctcat
    43081 ccatctgcaa ctgggaatca cacttctgct tatgcccgtg caaatcgtca tttttcatgg
    43141 cattagtctg acctcgttta ttgcaaatct attagcaatt cccttggtga catttatcac
    43201 ggttccgttg atcctcgccg cgatggttgt gcatttaagc gggccgttaa tcctggatca
    43261 agggttatgg tttcttgccg accggtcttt ggctttactt ttcttggggt taaagagttt
    43321 gccagaaggg tggatcaaca ttgctgaacg ttggcaatgg ctatcatttt ccccatggtt
    43381 cttactggtg gtatggcgat taaacgcctg gcgaacgttg ccagcaatgt gtgtggctgt
    43441 aggcttgctg atgtgctggc cgctgtggca aaaacctcga cctgacgagt ggcaagtgta
    43501 catgcttgat gtcgggcaag ggctggcaat ggtgatagcc agaaacggca aagcgattct
    43561 ctatgacacg ggactggcct ggcctgaagg ggatagtggg caacaactga ttatcccctg
    43621 gctccactgg cataatcttg aaccggaagg cgttatcctg agtcatgaac atctggatca
    43681 ccggggaggg ctggactcaa tattgcatac atggccgatg ttatggatca gaagtccgtt
    43741 aaactgggaa catcatcagc cctgtgtgcg tggcgaagcg tggcaatggc aaggattgcg
    43801 tttcagcgcg cactggcctt tacaaggtcg caacgataaa ggaaataacc attcctgtgt
    43861 ggttaaggtt gatgacggga cgaatagcat tcttctaacc ggtgatattg aagccccagc
    43921 tgaacaaaag atgctaagcc gttactggca gcaagtgcag gcaacattgc ttcaggtacc
    43981 tcaccatggc agtaatacct catcatcatt gccattaatt cagcgagtga atggaaaagt
    44041 ggcactcgca tcggcatcgc gctataacgc atggcgactg ccctctaaca aagttaagca
    44101 tcgctatcaa caacaaggtt atacgtggct tgatactcct catcaggggc aagtaacggt
    44161 cgatttttca gcgcaaggct ggcggattag cagcctcagg gagcaaattt tacctcgttg
    44221 gtatcatcag tggtttggcg tgccagtgga taacgggtag aatatgcggc tatttcaaca
    44281 aatgctggtt ttttgaatgc ataacgacaa agatctctct acgtggcaga cattccgccg
    44341 actgtggcca accattgcgc cttttaaagc gggtctgatc gtggcgggcg tagcgttaat
    44401 cctcaacgca gccagcgata ccttcatgtt atcgctcctt aagccacttc ttgatgatgg
    44461 ctttggtaaa acagatcgct ccgtgctggt gtggatgccg ctggtggtga tcgggctgat
    44521 gattttgcgt ggtatcacca gctatgtctc cagctactgt atctcctggg tatcaggaaa
    44581 ggtggtaatg accatgcgtc gccgcctgtt tggtcacatg atgggaatgc cggtttcatt
    44641 ctttgacaaa cagtcaacgg gtacgctgtt gtcacgtatc acctacgatt ccgaacaggt
    44701 tgcttcttcc tcttccggcg cactgattac tgttgtgcgt gaaggtgcgt cgatcatcgg
    44761 cctgttcatc atgatgttct attacagttg gcaattgtcg atcattttga ttgtgctggc
    44821 accgattgtt tcgattgcga ttcgcgtagt atcgaagcgt tttcgcaaca tcagtaaaaa
    44881 catgcagaac accatggggc aggtgaccac cagcgctgaa caaatgctga aaggccataa
    44941 agaagtattg attttcggtg gtcaggaagt ggaaacgaaa cgcttcgata aagtcagcaa
    45001 ccgaatgcgt cttcagggga tgaaaatggt ttcagcctct tccatctctg atccgatcat
    45061 tcagctgatc gcctctttgg cgctggcgtt tgttctgtat gcggcgagct tcccaagtgt
    45121 catggatagc ctgactgccg gtacgattac cgttgttttc tcttcaatga ttgcactgat
    45181 gcgtccgctg aaatcgctga ctaacgttaa cgcccagttc cagcgcggta tggcggcttg
    45241 tcagacactg tttaccattc tggacagtga gcaggagaaa gacgaaggta agcgcgtgat
    45301 cgagcgtgcg actggcgacg tggaattccg caacgtcacc tttacttatc cgggacgtga
    45361 cgtacccgca ttgcgtaaca tcaacctgaa aattccggca gggaaaacgg tcgctctggt
    45421 tggacgctct ggttcaggta aatcaaccat cgccagcctg atcacgcgtt tttacgatat
    45481 tgatgaaggc gaaatcctga tggatggtca cgatctgcgc gagtataccc tggcgtcgtt
    45541 gcgtaaccag gttgctctgg tgtcgcagaa tgtccatctg tttaacgata cggttgctaa
    45601 caacattgct tacgcacgaa ctgaacagta cagccgtgag caaattgaag aagcggcgcg
    45661 tatggcctac gccatggact tcatcaataa gatggataac ggtctcgata cagtgattgg
    45721 tgaaaacggc gtactgctct ctggcggtca gcgtcagcgt attgctatcg ctcgagcctt
    45781 gttgcgtgat agcccgattc tgattctgga tgaagctacc tcggctctgg ataccgaatc
    45841 cgaacgtgcg attcaggcgg cactggatga gttgcagaaa aaccgtacct ctctggtgat
    45901 tgcccaccgc ttgtctacca ttgaaaaggc agacgaaatc gtggtcgtcg aggatggtgt
    45961 cattgtggaa cgcggtacgc ataacgattt gcttgagcac cgcggcgttt acgcgcaact
    46021 tcacaaaatg cagtttggcc aatgatcgaa aaaatctggt ctggtgaatc ccctttgtgg
    46081 cggctattgc tgccactctc ctggttgtat ggcctggtga gtggcgcgat ccgtctttgc
    46141 tataaactaa aactgaagcg cgcctggcgt gcccccgtac cggttgtcgt ggttggtaat
    46201 ctcaccgcag gcggcaacgg aaaaaccccg gtcgttgtct ggctggtgga acagttgcaa
    46261 cagcgcggta ttcgcgtggg ggtcgtatcg cggggatatg gtggtaaggc tgaatcttat
    46321 ccgctgttat tgtcggcaga taccaccaca gcacaggcgg gtgatgaacc tgtgttgatt
    46381 tatcaacgca ctgatgcgcc tgttgcggtt tctcccgttc gttctgatgc ggtaaaagcc
    46441 attctggcgc aacaccctga tgtgcagatc atcgtaaccg acgacggttt acagcattac
    46501 cgtctggcgc gtgatgtgga aattgtcgtt attgatggtg tgcgtcgctt tggcaatggc
    46561 tggtggttgc cggcggggcc aatgcgtgag cgagcggggc gcttaaagtc agttgatgcg
    46621 gtaatcgtca acggcggtgt cccccgcagc ggtgaaatcc ccatgcatct gctgccgggt
    46681 caggcggtga atttacgtac cggtacgcgt tgtgacgttg ctcagcttga acatgtggtg
    46741 gcgatggcag ggattgggca tccgccgcgc ttttttgcca cgctgaagat gtgcggcgta
    46801 caaccggaaa aatgtgtacc gctggccgat catcagtctt tgaaccatgc ggatgtcagc
    46861 gcgttggtaa gcaccgggca aacgctggta atgactgaaa aagatgcggt gaaatgccgg
    46921 gcctttgcag aagaaaattg gtggtatttg cccgttgacg cacagctttc aggtgatgaa
    46981 ccagcgaaac tgcttgcgca actaacctcg ctggcttctg gcaactagtt acgccgcggc
    47041 agcgttcgat tgatggagtc atgaatgtcg ctgccgcacc tctcccttgc tgatgcgcgt
    47101 aatcttcacc ttgccgcgca aggcctgtta aacaaacccc gccgtcgagc gtcgttggag
    47161 gatattccgg caacgatctc ccgcatgtcc ttgctgcaaa tcgataccat caatattgtt
    47221 gcccgtagcc catatctggt gcttttcagt cgtctgggaa attatcctgc ccagtggctg
    47281 gatgagtctc tggcgcgtgg cgaattaatg gaatactggg cgcatgaagc ctgctttatg
    47341 ccacgcagtg actttcgtct tattcgccac cgcatgctgg cacctgaaaa aatgggctgg
    47401 aaatacaaag acgcctggat gcaggaacat gaggcggaaa ttgcacagtt aattcagcat
    47461 attcatgata aggggccggt acgttcagcc gattttgagt atcctcgtaa aggtgcaagc
    47521 ggctggtggg aatggaagcc gcataaacgg catctggaag gtttatttac tgccggaaag
    47581 gtgatggtga ttgaacggcg caacttccag cgcgtttatg atttaaccca ccgtgtcatg
    47641 cctgactggg atgatgagcg cgatctcgtt tcgcaaacag aagcagaaat catcatgctg
    47701 gataacagtg cgcgtagcca gggaatattc cgcgaacagt ggctggcaga ttactatcgg
    47761 ctgaaacgtc cggcactggc agcgtggcgc gaagcgaggg ctgaacagcg gcaaatcatt
    47821 gctgtgcatg ttgaaaaatt gggcaatctt tggctgcatg ctgatttgct gccgctactc
    47881 gagcgagcgc tggccggaaa gctcactgca acgcacagcg cggtactttc gccttttgat
    47941 cctgttgtct gggatcgcaa acgcgcagag cagctttttg attttagcta ccggctggag
    48001 tgctataccc cagcgccgaa acgccagtat ggctattttg ttctgccgtt attacatcgt
    48061 gggcaattag ttgggcgaat ggatgccaaa atgcatcgcc agacaggcat ccttgaagtt
    48121 atctctctgt ggttacagga aggtattaaa ccaacgacaa cgctgcaaaa agggttacgt
    48181 caggcgatta ctgatttcgc taactggcag caggcaacgc gggtgacatt aggacactgc
    48241 ccgcaaggtc tctttacgga ttgccgcacc ggctgggaaa tagaccccgt cgcataaatg
    48301 attatgataa gatttatgca ttcataaccc gatccatctg gaggaactat ggatcatcgt
    48361 ctgcttgaaa tcattgcctg cccggtttgc aacggaaaac tttggtataa ccaggaaaaa
    48421 caagaactta tttgcaaact ggacaacctg gctttcccgc tgcgcgatgg cattccagtg
    48481 ttgctggaaa cggaagcccg cgtgctgact gctgatgaga gtaaatcatg agttttgtgg
    48541 tcattattcc cgcgcgctac gcgtccacgc gtctgcccgg taaaccattg gttgatatta
    48601 acggcaaacc catgattgtt catgttcttg aacgcgcgcg cgaatcaggt gccgagcgca
    48661 tcatcgtggc aaccgatcat gaggatgtcg cccgcgccgt tgaagccgct ggcggtgaag
    48721 tatgtatgac gcgcgccgat caccagtcag ggacagaacg cttggcggaa gttgtcgaaa
    48781 aatgcgcgtt cagcgacgac acggtgatcg ttaatgtgca gggtgatgaa ccgatgatcc
    48841 ctgcgacaat cattcgtcag gttgctgata acctcgctca gcgtcaggtg ggtatggcga
    48901 ctctggcggt gccaatccac aatgcggaag aagcgtttaa cccgaatgcg gtgaaagtgg
    48961 ttctcgacgc tgaagggtat gcactgtact tctcacgcgc taccattccg tgggatcgtg
    49021 atcgttttgc aaaaggcctt gaaaccgttg gcgataactt cctgcgtcat cttggtattt
    49081 atggctaccg tgcaggcttt atccgtcgtt acgtcacctg gcagccaagc ccgttagaac
    49141 acatcgaaat gttagagcag cttcgtgttt tgtggtacgg cgaaaaaatc catgttgctg
    49201 tcgcgcatga agttcctggc acaggtgtgg atacccctga agatctcgag cgcgttcgcg
    49261 ctgaaatgcg ctaatttcac ttcacgacac ttcagccaat tttgggagga gtgtcgtacc
    49321 gttacgattt tcctcaattt ttcttttcaa caattgatct cattcaggtg acatctttta
    49381 tattggcgct cattatgaaa gcagtagctt ttatgagggt aatctgaatg gaacagctgc
    49441 gtgccgaatt aagccattta ctgggcgaaa aactcagtcg tattgagtgc gtcaatgaaa
    49501 aagcggatac ggcgttgtgg gctttgtatg acagccaggg aaacccaatg ccgctaatgg
    49561 caagaagctt tagtacgccc ggaaaagccc gacaactggc atggaaaacc accatgctgg
    49621 caagaagtgg gactgtccgt atgccgacta tttatggtgt gatgacgcat gaagaacacc
    49681 ccggccctga tgtcctgcta ctggagcgga tgcgtggtgt ttcggtagag gcaccagccc
    49741 gaacaccaga acgttgggaa cagctcaaag accaaatcgt tgaagcctta ctggcctggc
    49801 accgtcagga cagtcgcggt tgcgtcggcg cggtcgacaa tactcaggaa aatttctggc
    49861 cctcatggta ccggcaacat gttgaagtgc tatggaccac gctcaatcag ttcaataaca
    49921 ccggtctgac gatgcaggat aagcggatcc tgtttcgcac tcgcgaatgt ctcccggcat
    49981 tatttgaagg ttttaacgac aattgtgtgc tgattcacgg taacttctgt ttacgcagca
    50041 tgttgaaaga ttcgcgcagc gatcagttac tggcgatggt cgggccggga ctaatgcttt
    50101 gggcaccgcg agaatacgaa ctgttccgac taatggataa ttctctggcg gaagatttgc
    50161 tctggagtta cctgcaacgc gcgccagtgg cggagtcgtt catctggcgg cgttggttgt
    50221 atgtgttatg ggatgaagtt gcgcaactgg ttaataccgg acgatttagt cggcgcaact
    50281 tcgatctggc atcaaaatca ctcttgccgt ggctcgcctg acgaaccttt tagccactgc
    50341 cagatgcggc caagcgtttc atagccaaca cgatcgctat gcatcaacca gaccggagaa
    50401 gggattgccc gttcccacgg gttaagcggc gagtcgatgg ccaactgatt cgccggggct
    50461 ggtagtggat ttaacccttc ctgctgaaaa aagatcatcg cccgcggcag atgtgatgcg
    50521 gaggtcacca gcaggaaagg ggcgtcacca atcgcctgtt tcactgccgc agcttcttct
    50581 tcggtatctt ttggcaaatc cagggtgata atttgctcgc gcggtacgcc cagcgattgc
    50641 gcaactctgg cacctacttc cgctgtactc accgtattgg tttttgctac gcctcccgtg
    50701 aagatcagtt ttgaacccgg attttcgcgc cataaacgga taccctcatt caggcgtggc
    50761 agactgttat tgattaaatt agagctcggt gcccactgcg ggttccaggt ataaccacct
    50821 ccgagcacca cgatatagtc cactttttgc gaattattcc atgtcggata ggtgctttcg
    50881 atgggacgca gcaggcggtc ggctaccggt tgcaaactca ataacaagag tgccagccac
    50941 ccaatgctaa taaaaatctt cccggttttt tgaaaacggc taaaccacag tagcgccagg
    51001 ccagcgccaa taattaacaa catcagcgga agtggcagca acatattgcc aatcactttt
    51061 ttcagtgtaa aaagcatcct ttctggttcc ttttttaacc atatagcaag ggatcgccag
    51121 cgatattaca ccagacgggt tcattctcgc cgtggctgtg acaaaatagc gtctttatca
    51181 gaaaacgtct tagccaaccg gtggagaaag caatgcagga tcgcaatttt gatgatattg
    51241 cggaaaagtt ttcccgtaac atttacggca ccaccaaagg gcagcttcga caggctattc
    51301 tgtggcagga tctcgatcgc gtgctggcgg aaatgggtcc gcaaaaactg cgtgtgctgg
    51361 atgcgggcgg tggagaaggg cagaccgcaa tcaaaatggc cgagcgcggg catcaggtca
    51421 ttttatgcga tctttctgcg cagatgatcg accgcgcaaa acaggcggca gaagcaaaag
    51481 gtgtgagcga caacatgcaa tttatacatt gcgccgctca ggatgttgct tcgcatttgg
    51541 aaacgcccgt tgatctgata ttgttccatg cggtgctcga gtgggtggct gatccccgca
    51601 gcgtattgca gaccctgtgg tcagtgttgc gtccaggcgg cgtgttgtcg ttaatgttct
    51661 acaatgcgca tggtttgttg atgcataaca tggtcgccgg gaattttgat tacgtgcagg
    51721 cgggaatgcc gaaaaagaaa aaacggacgc tttcgccaga ttatccacgc gacccgacgc
    51781 aggtttatct gtggctggaa gaagctggtt ggcaaattat gggtaagaca ggcgttcgcg
    51841 tgtttcatga ttatctgcgc gagaagcacc agcagcgcga ctgctatgaa gcattacttg
    51901 aattagaaac gcgttattgc cgtcaggaac cgtatattac cctggggcgt tatattcatg
    51961 tcaccgcgcg caaaccgcag agcaaggata aagtatgagt gaattttccc agacagtccc
    52021 cgaactggtt gcctgggcca gaaaaaatga cttctccatt tcgctgccgg tagaccgact
    52081 ctcttttctg ctggcagttg ccacgctgaa cggcgagcgt ctggatggtg agatgagtga
    52141 aggcgagctg gtggatgcat tccgccatgt gagtgatgcg tttgagcaaa ccagcgaaac
    52201 catcggcgtg cgcgccaaca acgcgatcaa cgacatggtg cgtcaacgtc tgctgaaccg
    52261 ctttaccagc gagcaggcag aagggaacgc aatttaccgt ctgacgccgc tcggcatcgg
    52321 cattactgac tattacatcc gtcagcgcga gttttctacg ctgcgccttt ctatgcagtt
    52381 gtcgattgtg gcgggtgagc tcaaacgcgc agcagatgcc gccgaagagg gcggtgatga
    52441 atttcactgg caccgtaatg tctatgcgcc actgaaatat tcggtagcag aaattttcga
    52501 cagtatcgac ctgacgcaac gtctgatgga cgaacagcag cagcaggtga aggacgatat
    52561 cgcccagttg ctgaacaaag actggcgggc ggcgatttcc agctgtgaat tgttgctttc
    52621 ggaaacttcc ggaacgctgc gtgaattgca ggatacactg gaagcggcag gcgacaaatt
    52681 gcaggctaat ctgttgcgca ttcaggatgc gacgatgact catgacgatc tgcatttcgt
    52741 cgatcgtctg gtgttcgatc tgcagagcaa actcgaccgt attatcagtt ggggccagca
    52801 atccatcgat ttgtggattg gctacgaccg ccacgtacat aaatttattc gtaccgcgat
    52861 cgatatggat aaaaaccgcg tctttgctca gcggttacgt cagtcggtac aaacctattt
    52921 tgatgagccg tgggcgctaa gttatgccaa tgccgatcgt ctgctggata tgcgtgacga
    52981 agagatggca ctgcgcgatg aagaagtgac tggggaactt ccgccagatt tggaatacga
    53041 agagtttaac gagatccgcg aacagctggc ggcgatcatc gaagaacaac ttgccgtgta
    53101 caaaaccaga caagtgccgc tggatcttgg tctggtggta cgcgaatatc tgtcacagta
    53161 tccgcgtgca cgtcactttg acgttgcgcg cattgttatt gatcaggcgg tacgtcttgg
    53221 cgtagcgcaa gcagatttca ccggactgcc agcgaaatgg cagccgatta atgattacgg
    53281 agccaaggta caggcgcatg tcatcgacaa atattgaaca agtgatgccg gttaagctgg
    53341 cgcaggcgct ggcgaatccg ttatttccgg cgctggacag cgccttacgt tcaggccgcc
    53401 atattggcct cgacgaactg gataatcatg cattcctgat ggattttcag gaatatctgg
    53461 aagagtttta cgcgcgttat aacgttgagc ttattcgtgc accggaaggg ttcttctatt
    53521 tacgtccacg ttccaccacg ctgatccctc gttccgtctt gtcggaactg gatatgatgg
    53581 tcgggaaaat cctctgttat ctctatctca gcccggaacg gctggcgaat gaggggattt
    53641 tcacccagca ggaactgtac gacgaactgc tcaccctggc cgatgaagcg aaactgctga
    53701 aactggtgaa caaccgttca accggttcag acattgaccg tcagaagttg caggaaaaag
    53761 tacgttcttc gctcaaccgt ctgcgtcgtt taggtatggt gtggtttatg ggccacgaca
    53821 gcagcaagtt ccgcattacc gaatcggtgt tccgcttcgg agccgatgtg cgtgctggcg
    53881 acgatccccg tgaagcacag cgtcgcctga ttcgtgatgg cgaagcaatg ccgattgaaa
    53941 atcatctgca actcaacgat gaaaccgaag agaatcagcc agatagcgga gaggaagaat
    54001 aatgattgaa cgcggtaaat ttcgctcact gacgctgatt aactggaacg gcttctttgc
    54061 ccgaactttt gaccttgacg agctggtcac gacgctttct ggcggtaacg gggcgggtaa
    54121 atccaccacc atggcggcgt tcgttacggc gctgatcccc gacctgaccc tgctgcattt
    54181 ccgtaacact acggaagccg gggccaccag cggttcgcgc gataaaggtc tgcacggtaa
    54241 gctgaaagcg ggtgtctgtt attcgatgct cgacaccatt aactcgcgcc accagcgcgt
    54301 ggtggtcggt gtgcgtctgc aacaggttgc cggacgcgat cgtaaagtgg atatcaaacc
    54361 atttgccatt cagggactgc cgatgtcggt gcagccgaca cagctggtga ccgaaactct
    54421 gaacgaacgc caggcgcgcg tgctgccgct taacgagctg aaagacaagc tcgaggcgat
    54481 ggaaggcgtg cagtttaaac agttcaactc cattaccgat taccactcgc tgatgttcga
    54541 tctgggcatc attgcgcgtc gtctgcgctc cgcatctgac cgtagcaaat tctatcgtct
    54601 gatcgaagct tcgctgtatg gcgggatctc cagtgccatt acccgttctc tgcgcgacta
    54661 cctgttgccg gaaaacagcg gcgtgcgtaa agcgttccag gacatggaag cggcgctgcg
    54721 tgaaaaccgc atgacgctgg aagcgattcg tgtcacccag tcggaccgtg acctgtttaa
    54781 gcatctgatc agcgaagcta ccaactacgt ggcggcggac tacatgcgtc acgccaacga
    54841 gcgccgtgtc catctcgata aggccctgga gtttcgtcgc gaactacata cttcgcgtca
    54901 gcaactggcg gcggagcagt acaaacacgt cgatatggcg cgtgagctgg cagagcacaa
    54961 cggtgccgaa ggcgatctgg aagcggatta tcaggcggcc agcgatcacc tgaacctggt
    55021 gcaaaccgca ctgcgtcagc aggagaagat cgaacgctac gaagcggatc tcgatgagct
    55081 gcagatccgt ctggaagagc aaaacgaagt ggtggcagaa gccatcgaac gccaggaaga
    55141 gaacgaggct cgcgcggaag ctgccgaact ggaagtggac gagctgaaaa gccagcttgc
    55201 tgactaccag caggcgctgg acgtccagca aacgcgcgcg atccagtaca accaggcgat
    55261 tgctgcgctt aatcgtgcca aagaactgtg ccatctgccg gacttaaccg ccgacagcgc
    55321 cgccgaatgg ctggaaacct tccaggcgaa agagctggaa gcgactgaaa aaatgctctc
    55381 tcttgagcag aaaatgagca tggcgcaaac cgcgcacagc cagtttgagc aggcttatca
    55441 gctggtggtg gcaatcaacg gcccgctggc gcgtaacgag gcgtgggatg tcgctcgcga
    55501 actattgcgc gaaggggtcg atcagcgtca cctggcagag caggttcagc cgttgcggat
    55561 gcgcttaagc gaactggaac agcgtctgcg cgaacagcaa gaagctgagc gtctgctggc
    55621 agatttctgc aaacgtcagg gcaagaattt tgacattgac gagctggaag ccctgcatca
    55681 ggagctggaa gcacgcattg cctctctttc cgatagcgtg tctaacgccc gtgaagagcg
    55741 gatggcactg cgccaggagc aggaacagct gcagtctcgc attcagagtt tgatgcagcg
    55801 tgcaccggtt tggctggcag cgcaaaacag tctcaaccag ttgagcgaac agtgcggcga
    55861 agagttcacc tccagccagg acgtcactga atatctgcaa cagttgctgg agcgtgagcg
    55921 cgaggccatt gttgaacgcg atgaagtagg cgcgcgcaaa aacgccgtcg atgaagagat
    55981 cgaacgttta agccagcctg gcggctctga agatcagcgt ctgaacgcgc tagcggagcg
    56041 ttttggtggt gtgctgctgt cagaaattta tgacgacgtt agcctggaag atgcgccgta
    56101 cttctcagcg ctgtatggcc cgtcacgcca cgccatcgtg gtgccagatc tgtcacaggt
    56161 aaccgaacac ctggaaggtt tgaccgattg cccggaagat ctctatttga tcgaagggga
    56221 tccgcagtca ttcgatgaca gcgtgttcag cgttgatgag ctggaaaaag cggtagtggt
    56281 gaaaatcgcc gatcgtcagt ggcgttattc acgtttcccg gaagtgccgc tgtttggtcg
    56341 tgctgcgcgt gaaagccgta ttgaaagcct ccatgccgag cgtgaagtgc tttccgaacg
    56401 cttcgccacg ctctcctttg atgtacagaa aacccagcgt ctgcatcagg cgttcagccg
    56461 ctttatcggc agtcatctgg cggtggcgtt tgagtctgac ccggaagcag aaatccgtca
    56521 actgaacagc cgtcgcgtcg aactggagcg ggcgttgagt aatcatgaaa atgataacca
    56581 gcagcagcgt attcagtttg agcaggcgaa agaaggcgtt acggcgctga accgcattct
    56641 gccgcgtctc aacctgttgg ctgatgacag cctggcggat cgcgtcgatg aaatccgcga
    56701 acgtctggat gaagcccagg aagccgcgcg ttttgttcag cagtttggca atcaactggc
    56761 gaaactggaa ccgatcgttt cggtattgca gagcgacccg gaacagttcg aacagttaaa
    56821 agaagattac gcgtactctc agcagatgca gcgcgatgcc cgtcagcagg cgtttgccct
    56881 gacggaagtg gtgcagcgtc gtgcgcactt tagctactct gactcggcag aaatgcttag
    56941 cggtaacagc gatctcaacg aaaaactgcg tgaacgtctg gaacaggcgg aagcggagcg
    57001 tacccgcgct cgcgaagcgt tgcgcggtca cgcagcgcag ttgagtcagt acaaccaggt
    57061 gctggcttcg ctgaaaagtt cttacgacac caaaaaagag ctactcaatg atctgcaacg
    57121 tgaattgcag gatatcggcg tgcgtgctga tagcggggca gaagagcggg cacgtattcg
    57181 ccgtgacgag ctgcatgcac aactgagcaa taaccgttcg cgccgcaatc aactggaaaa
    57241 agcgctcacc ttctgcgaag cggagatgga taacctgacc cgcaaactgc gcaagctgga
    57301 gcgtgattac tttgagatgc gcgagcaggt agtgaccgcg aaagcgggct ggtgtgcagt
    57361 catgcgcatg gtgaaagata acggcgttga gcgccgctta caccgtcgtg agctggctta
    57421 tctctccgct gatgatttgc gttccatgtc ggataaggcg ttaggtgcgc tgcgtctggc
    57481 ggtggcggat aacgaacatc tgcgcgacgt gctgcgcatg tcggaagatc ccaaacgtcc
    57541 ggagcgtaaa attcagttct tcgtggcggt ttatcagcat ctgcgtgaac gtattcgtca
    57601 ggatattatt cgtaccgatg atccggtgga agctatcgaa cagatggaga tcgaacttag
    57661 ccgtctgacc gaagagttaa cctcccgtga acagaaactg gcgatcagtt cccgcagcgt
    57721 ggcgaacatc attcgcaaaa ccattcagcg cgagcagaac cgtatccgta tgctcaacca
    57781 ggggttgcag aacgtatcgt ttggtcaggt gaacagcgtg cgcctcaacg tgaacgtgcg
    57841 tgaaacgcac gccatgctgc tggatgtgct ctctgaacag catgagcagc atcaggatct
    57901 gtttaacagc aaccgtctga ccttctcgga agcgctggcg aaattgtatc agcgtcttaa
    57961 tccgcagatt gatatggggc agcgcacgcc gcagaccatt ggcgaagaac tgctggatta
    58021 ccgcaactat ctggaaatgg aagttgaggt taaccgtggt tccgatggct ggttgcgcgc
    58081 agagtctggt gcattgtcga ccggtgaggc gattggtacc ggtatgtcga ttctggtgat
    58141 ggtggtacaa agctgggaag atgaatctcg ccgcctgcgc ggtaaagata tctctccttg
    58201 ccgcctgctg ttcctcgatg aagcagcgcg actggatgct cgttctatcg ccacgctgtt
    58261 tgaattgtgt gagcgtttgc aaatgcagct catcatcgca gcgccggaaa atatcagccc
    58321 ggagaaaggc accacctata aactggtgcg taaagtcttc cagaataccg aacatgttca
    58381 tgtcgtcggc ctgcgaggat ttgcgccgca actccctgaa acgcttccag gaagtgacga
    58441 agcgccttct caggcgagtt aaaattaagg cggcagcaat gccgcctttt ctttttccga
    58501 aaactccgtt tctgcactaa aaaagtggca cattacggcg cgcttttctt taaacttctt
    58561 tacattaggt tatgtaaaaa cgtatcggcg tttatatact gaagataagc ctgatgagta
    58621 acaggcttgc tcgtcatact ttcgtgagta ttggcgttgt acaggcaagt cgtaaaataa
    58681 cagcctggct attcagagta tgataaaaac agggggcaag ggatgttgct taatatgatg
    58741 tgtggtcgtc ggctgtcggc aatcagtttg tgcctggccg taacattcgc tccactgttc
    58801 aatgcgcagg ccgatgagcc tgaagtaatc cctggcgaca gcccggtggc tgtcagtgaa
    58861 cagggcgagg cactgccgca ggcgcaagcc acggcaataa tggcggggat cctgccattg
    58921 cctgaaggtg cggcagaaaa agcccgcacg caaatcgaat ctcaattacc cgcaggttac
    58981 aagccggttt atcttaacca gcttcaactg ttgtatgccg cacgcgatat gcaacccatg
    59041 tgggaaaacc gtgatgctgt taaagccttc cagcaacagc tggcagaggt ggcgattgcc
    59101 ggtttccagc cgcagtttaa taaatgggta gagttactga ccgatcctgg tgttaacggg
    59161 atggcacgcg acgtggtgct ctctgatgcg atgatgggct atctccattt cattgcaaat
    59221 attccggtca aaggcactcg ctggctatat agcagtaaac cttatgcgct tgcaacgccg
    59281 ccgctttcgg tgattaacca atggcagcag gcgctggata aaggtcaatt gcctacgttt
    59341 gttgcaggac tggcaccgca gcatccgcaa tatgcggtga tgcatgaatc gttactggcc
    59401 ttactcagtg acaccaaacc gtggccccaa ctgaccggca aagcaacgtt gcgcccaggg
    59461 cagtggagta acgacgtacc ggcgttgcgt gaaatattgc aacgcacagg catgttggac
    59521 ggggggccga aaattactct acctggcgat gacacgccaa ctgacgcggt agtcagccca
    59581 tccgctgtta ctgttgaaac agcagaaact aagccgatgg ataagcaaac gacgtctcgt
    59641 agtaaacctg cgcctgccgt tcgcgccgcc tacgataatg aactggtgga agccgttaaa
    59701 cgttttcagg catggcaagg attgggggca gatggtgcta ttggcccggc aacgcgtgac
    59761 tggttaaacg taacgcccgc ccagcgtgct ggcgtgttgg ctctcaacat ccagcgattg
    59821 cgcttgctgc caacagagct ttctaccggg atcatggtta acattccggc ctattcgctg
    59881 gtctactatc agaacggcaa tcaggtgctg gattcgcgag tcattgtcgg tcgccccgat
    59941 cgcaaaacgc cgatgatgag cagtgccctt aacaatgtag tggtaaaccc gccgtggaac
    60001 gtacctccaa ctctggcacg caaagatatt ctgccgaaag tgcgcaacga tccgggatat
    60061 ctcgaaagcc atggctatac ggtgatgcgc ggctggaaca gcagagaagc gattgaccca
    60121 tggcaggttg actggtctac aatcacggcc tcgaatttac cgtttcgctt ccaacaggct
    60181 ccaggcccac ggaactcgct ggggcgctat aaattcaata tgccgagttc agaggccatt
    60241 tatttgcatg acacgccgaa ccacaatctg ttcaagcgtg atacacgcgc attgagctca
    60301 ggctgtgtac gagtgaataa agcttccgat ctggcgaata tgctgttgca ggatgcaggc
    60361 tggaatgaca aacgtatttc tgatgcgctg aagcagggtg atacacgtta cgtcaatatt
    60421 cggcagtcga ttccggtgaa tctctactac ctgacggcct ttgttggtgc agatggtcgt
    60481 acccagtatc gtacagatat ttacaattat gatctgcctg cgcgatccag ctcgcaaatc
    60541 gtatcgaaag cggaacaatt aatcaggtaa atgaagtagt tcggggaaat cagttgtcgt
    60601 aataattagc atgattgggg gcaattctct gcagcccccg tcactgctgg ggttgagtca
    60661 tcttgacgtc tgctttacgg gcggttaagg tgcctcttgt gcgccagaag tgcatataaa
    60721 cgataacatt gacctgtaga cttgattatc atggacaaat tcgacgctaa tcgccgcaaa
    60781 ttgctggcgc ttggtggcgt tgcactcggt gccgccatcc tgccgacccc tgcgtttgca
    60841 acactctcta ccccacgccc gcgcattttg acactcaata atcttcatac cggagagtca
    60901 atcaaagcgg agtttttcga tggcagaggc tatattcagg aagaattggc aaaacttaac
    60961 cattttttcc gcgattaccg cgcgaacaaa ataaagtcca tcgacccagg attattcgac
    61021 cagttgtatc gcctgcaagg gttattaggc acccgcaaac cggtgcaact catttccggt
    61081 tatcgttcca ttgataccaa caatgaacta cgcgcccgca gccgtggagt agcgaagaaa
    61141 agctatcaca ctaaaggcca ggcgatggat ttccatattg aaggtatcgc gttaagcaat
    61201 attcgcaaag ccgcgttatc tatgcgcgca ggtggtgtag gatattaccc acgtagtaac
    61261 tttgtgcata ttgataccgg gccagcacgg cactggtagt aattgcttaa cgaaacaggg
    61321 gcagtatgaa ctatcgtatt attccggtca ccgcattctc ccagaactgt tcattgatct
    61381 ggtgtgaaca aacccgcctg gccgcactgg tcgatcctgg cggcgatgcg gaaaaaatca
    61441 aacaggaagt tgacgccagc ggcctgacac tgatgcagat cctgctgacg catggtcatc
    61501 tggaccacgt tggcgcagcg gcggaactgg cgcaacatta cggcgtgccg gtttttggcc
    61561 cggaaaaaga agatgagttc tggctgcaag gcttgcctgc gcaaagtcgt atgtttggtc
    61621 tggaagagtg ccagccgctg acgccagatc gttggctgaa cgaaggcgat accatcagca
    61681 tagggaatgt gactttacag gtgttacatt gccctgggca tacgccgggt catgtcgtgt
    61741 tttttgatga tcgggcaaag ctgctgattt ctggcgatgt tattttcaaa ggcggagtag
    61801 ggcgcagtga cttcccgcgt ggcgatcata atcaactgat ttcttcaatc aaagataaat
    61861 tgctgccact gggtgatgac gtgacattta ttccgggtca cggaccatta tccacacttg
    61921 gttatgaacg cctgcataat cccttcctgc aagacgaaat gcccgtctgg taaggcacac
    61981 ataaaaaagc ccgcttttca gcgggcttca ttgtttttaa cgcttacagc actgccacaa
    62041 tcgcttcgca cagcggagcc atgttatctg gtgtcatccc ggccacgttt acgcgaccag
    62101 aagcaacagc atacacgcca aactcttcgc gcagacgcag cacttgttct tttgtcaggc
    62161 cactgaagga gaacatgccg ttctgtttga tgataaagct gaagtcgcgg tttgcgcctt
    62221 tttcctgcag cgtattgacg aacaactgac gcatacgctg aatacgctgg cgcatatcag
    62281 tcagctcttg ttcccaaatc gcacgtaacg catcgttgct caggatggtg gcaacaacag
    62341 aagcgccgtg tgctggtggg ttagagtagt tagcgcgaat cgccgctttc atttggctga
    62401 atgcgcgatc aacggtttca ctgtcggcag caaccagagt acaagcgcca acacgctcgt
    62461 tgtacaggcc aaagttttta gagtaggaac tggcaacaat cagctcttta tgcatagccg
    62521 cgaaagcgcg cagtccttca gcatcttctt ccagaccacg ggcaaaaccc tggtaagcga
    62581 agtcaaacag cggtaaccag cctttctcaa cggagagttg tgccagtgtc tgccattgtt
    62641 ccagcgtagg gtcgataccg gttgggttgt ggcagcagcc atggaacagc actacgtcgc
    62701 cagcctgagc ttcgttcagg ctgttaatca gtgcatcgaa gtcaagggtg tggttttccg
    62761 catcataata agcgtattca cgaacttcca gatctgcaga gttaaagacg ctcttatggt
    62821 tcggccagct tgggttgctc acccacactc gcttaacgct ggtatttttt gccaggaaat
    62881 cggcagctat gcgtagtgcg ccagtgccac ccggagtctg tgccgtgcga gcacgtttgt
    62941 cattgatcag ggcgctacct ttaccaaaca gcagttcctg agtgcagcga ccaaattcag
    63001 ggatgccgtc aatgccgagg taatttttgg tggtttcatt ttcgagcaga tactgttcag
    63061 cctttttcac gctggtcagt accggggttt tgcccgtctc atctttatag acaccaatcc
    63121 cgaggttaat tttgccggga cgttcatcgg cacgaaacag atcggccagg cccagaatcg
    63181 ggtcggcagg agcggcggta atgttctcaa acatgacgag gttccattat ggttacagaa
    63241 gggaagtccg ctatcagggt aacgggagat ttacaaaatt ccaactatta ctgatgaaaa
    63301 cgcaggctgt ttttgcaaga cgtgagattg ctctggaagg cataaaaaaa acaggaccaa
    63361 agtcctgttt tttcggcatt taacaaagag gtgtgctatt agaactggta aacgataccc
    63421 acagcaacgg tgtcgtctga acctacgccc agtttgttgt cagaatcgat ctggttgatg
    63481 atgtagtcaa cataggtgga catgtttttg ttgaagtagt aggttgcgcc cacttcaaag
    63541 tagttcacca gatcaacatc accgatacct tctacgtctt tcgctttaga tttggtgtaa
    63601 gcgatggacg gacgcagacc gaaatcgaac tggtattgcg caactaacag aacgtcttgc
    63661 gttttgttgg cgaagccgct gatgtttgta aatttattag tgatcggcgt agcgttacgc
    63721 gtttcaccgt agttcgctgc caggtagatg ttgttcgcat catacttcag accagtagcc
    63781 cactgttcag cttttttacc gttgccaaga agttgagctt cttgcaggtt ggtacggtca
    63841 gctgcaccat aagcaccaac gataccaaag ccttcgtatt cgtagctgat agaaccgcca
    63901 acaccgtcgc cgttagagcg gcgtgcagtg tcacgctcgt ttttacccag gtactgaaca
    63961 gcgaagttca ggccatcaac cagaccaaag aagttggagt tacgataggt agcaacgccg
    64021 ccaacacgac caacgaagaa gtcatcgctg tatgcagtat caccaccaaa ttctggcagc
    64081 atatcggtgt aacccagtgc atcataaacc acaccgtagt tacggccgta atcgaaagaa
    64141 ccaacgtcag cgtatttaag acccgcgaat gccagacgcg ttttgttacc agtttgagcg
    64201 tcagcgcctt cagagttgtt accctggaag ttatattccc actgaccata accggtcaga
    64261 tcggaattga tttgagtttc ccctttaaaa ccaagacggg cataggtcat gtcgccattg
    64321 ccaccgtaac tgttttcacc gttacccttg gagaaataat gcagaccgac agctttaccg
    64381 tacagatcta ctttgttgcc atctttgtta tagatttctg cagcgtttgc agtacctgct
    64441 actaacagag cagggacgat cactgccaga atattgcgct tcatcattat ttattaccct
    64501 catggttttt tttatgacac ctgccactgc cgtcaataat ttctgtcaat aaaattttac
    64561 ggaactattg atgagagttt ggtgtcttta tgtgtctgca ggcatctttc cattcaaact
    64621 aacgtttcgc taccgtgaaa gtgctacaaa gataaagatt tagtttcaaa aagaaaaaat
    64681 atgtaactaa aagtaaaaat taaggaactt tgtga
//
LOCUS       DAZFAB010000016        63437 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZFAB010000016 DAZFAB010000000
VERSION     DAZFAB010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 63437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.016"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            24..878
                     /gene="gatY"
                     /locus_tag="ACHJWA_001180"
     CDS             24..878
                     /gene="gatY"
                     /locus_tag="ACHJWA_001180"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFO4307824.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKHYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            907..2169
                     /gene="gatZ"
                     /locus_tag="ACHJWA_001181"
     CDS             907..2169
                     /gene="gatZ"
                     /locus_tag="ACHJWA_001181"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFO4307825.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFAIADKVGFARERIILGGDHLGPNCWQQENVDAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            2179..2631
                     /gene="gatA"
                     /locus_tag="ACHJWA_001182"
     CDS             2179..2631
                     /gene="gatA"
                     /locus_tag="ACHJWA_001182"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFO4307826.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            2662..2946
                     /gene="gatB"
                     /locus_tag="ACHJWA_001183"
     CDS             2662..2946
                     /gene="gatB"
                     /locus_tag="ACHJWA_001183"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFO4307827.1"
                     /translation="MKRKIIVACGGEVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            2950..4305
                     /locus_tag="ACHJWA_001184"
     CDS             2950..4305
                     /locus_tag="ACHJWA_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFO4307828.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLA
                     E"
     gene            4353..5393
                     /gene="gatD"
                     /locus_tag="ACHJWA_001185"
     CDS             4353..5393
                     /gene="gatD"
                     /locus_tag="ACHJWA_001185"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFO4307829.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            5499..6272
                     /locus_tag="ACHJWA_001186"
     CDS             5499..6272
                     /locus_tag="ACHJWA_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO4307830.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSDVDKLALQKTRVKLITV"
     gene            complement(6354..7253)
                     /gene="yegS"
                     /locus_tag="ACHJWA_001187"
     CDS             complement(6354..7253)
                     /gene="yegS"
                     /locus_tag="ACHJWA_001187"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFO4307831.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            7659..7976
                     /gene="yegR"
                     /locus_tag="ACHJWA_001188"
     CDS             7659..7976
                     /gene="yegR"
                     /locus_tag="ACHJWA_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFO4307832.1"
                     /translation="MKKIAAISLISVFLMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            complement(8306..9667)
                     /gene="yegQ"
                     /locus_tag="ACHJWA_001189"
     CDS             complement(8306..9667)
                     /gene="yegQ"
                     /locus_tag="ACHJWA_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFO4307833.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(9815..10147)
                     /gene="yegP"
                     /locus_tag="ACHJWA_001190"
     CDS             complement(9815..10147)
                     /gene="yegP"
                     /locus_tag="ACHJWA_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFO4307834.1"
                     /translation="MAGWFELSKSSDSQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            complement(10338..11060)
                     /gene="baeR"
                     /locus_tag="ACHJWA_001191"
     CDS             complement(10338..11060)
                     /gene="baeR"
                     /locus_tag="ACHJWA_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFO4307835.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLSYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            complement(11057..12460)
                     /gene="baeS"
                     /locus_tag="ACHJWA_001192"
     CDS             complement(11057..12460)
                     /gene="baeS"
                     /locus_tag="ACHJWA_001192"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFO4307836.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            complement(12457..13872)
                     /gene="mdtD"
                     /locus_tag="ACHJWA_001193"
     CDS             complement(12457..13872)
                     /gene="mdtD"
                     /locus_tag="ACHJWA_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4307837.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAITGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSSTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            complement(13873..16950)
                     /gene="mdtC"
                     /locus_tag="ACHJWA_001194"
     CDS             complement(13873..16950)
                     /gene="mdtC"
                     /locus_tag="ACHJWA_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFO4307838.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGMVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKSKQTVTE"
     gene            complement(16951..20073)
                     /gene="mdtB"
                     /locus_tag="ACHJWA_001195"
     CDS             complement(16951..20073)
                     /gene="mdtB"
                     /locus_tag="ACHJWA_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFO4307839.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYSGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQISDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            complement(20073..21320)
                     /gene="mdtA"
                     /locus_tag="ACHJWA_001196"
     CDS             complement(20073..21320)
                     /gene="mdtA"
                     /locus_tag="ACHJWA_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFO4307840.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLTNARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLLFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            21600..21656
                     /locus_tag="ACHJWA_001197"
     CDS             21600..21656
                     /locus_tag="ACHJWA_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO4307841.1"
                     /translation="MMKILIIVVLLVISYPAY"
     gene            21928..21984
                     /locus_tag="ACHJWA_001198"
     CDS             21928..21984
                     /locus_tag="ACHJWA_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO4307842.1"
                     /translation="MMKWIIIVVLLVISFPAY"
     gene            22256..22315
                     /gene="ibsA"
                     /locus_tag="ACHJWA_001199"
     CDS             22256..22315
                     /gene="ibsA"
                     /locus_tag="ACHJWA_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFO4307843.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            22536..23195
                     /gene="yegL"
                     /locus_tag="ACHJWA_001200"
     CDS             22536..23195
                     /gene="yegL"
                     /locus_tag="ACHJWA_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO4307844.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMNGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADIKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            23192..23953
                     /gene="pphC"
                     /locus_tag="ACHJWA_001201"
     CDS             23192..23953
                     /gene="pphC"
                     /locus_tag="ACHJWA_001201"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HFO4307845.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAMLAINEAMAYVSQKVQHGEFGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDVVSRLETFTSTERAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLAAATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLTLALWLP"
     gene            23950..25890
                     /gene="yegI"
                     /locus_tag="ACHJWA_001202"
     CDS             23950..25890
                     /gene="yegI"
                     /locus_tag="ACHJWA_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HFO4307846.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQSYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLPSFVGFERTANHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDIAHFRYAYASDNQRRGLKPPPRSIPLSMLPGDVEAMFQQ
                     AFTESGVATARPTAKAWVAALDLLRQQLKKCTVSAMHVYPGHLTDCPWCALDNQGVIY
                     FIDLGEEVITTSGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILIEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKVYKAEIQQRREAFNRA
                     KMDYDHLVSQIQQLGGLEGFIAKRAMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDAASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAVTPAERQAVMAKMAAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(25903..27255)
                     /gene="yegD"
                     /locus_tag="ACHJWA_001203"
     CDS             complement(25903..27255)
                     /gene="yegD"
                     /locus_tag="ACHJWA_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO4307847.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARIQEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            27389..28237
                     /gene="alkA"
                     /locus_tag="ACHJWA_001204"
     CDS             27389..28237
                     /gene="alkA"
                     /locus_tag="ACHJWA_001204"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFO4307848.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADDYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSADLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            complement(28338..31656)
                     /gene="dgcE"
                     /locus_tag="ACHJWA_001205"
                     /pseudo
     CDS             complement(28338..31656)
                     /gene="dgcE"
                     /locus_tag="ACHJWA_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            31974..32615
                     /gene="udk"
                     /locus_tag="ACHJWA_001206"
     CDS             31974..32615
                     /gene="udk"
                     /locus_tag="ACHJWA_001206"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFO4307849.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            32707..33288
                     /gene="dcd"
                     /locus_tag="ACHJWA_001207"
     CDS             32707..33288
                     /gene="dcd"
                     /locus_tag="ACHJWA_001207"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFO4307850.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDESEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            33310..35163
                     /gene="asmA"
                     /locus_tag="ACHJWA_001208"
     CDS             33310..35163
                     /gene="asmA"
                     /locus_tag="ACHJWA_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFO4307851.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESRKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMADTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(35216..35506)
                     /locus_tag="ACHJWA_001209"
     CDS             complement(35216..35506)
                     /locus_tag="ACHJWA_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4307852.1"
                     /translation="MKIETFDSVWDAVSDTPEQAENMRIRAELVTIINNWIEQQGFSQ
                     AQAASALGVTQPRISELARGKIQIFSIDKLITMMAHAGLHIQRIEVQYPHAA"
     gene            complement(35496..35834)
                     /locus_tag="ACHJWA_001210"
     CDS             complement(35496..35834)
                     /locus_tag="ACHJWA_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO4307853.1"
                     /translation="MRKKLAFLDTSLDDLRAFPESSRQEIGYQLDRIQQGLNPYDWKP
                     FSTIGPGVREIRTRDADGIYRVMYVAKFEEAVYVLHCFQKKTQTTSQSDIDLAKRRYK
                     ELVQERKNEN"
     gene            complement(35909..37492)
                     /gene="yegH"
                     /locus_tag="ACHJWA_001211"
     CDS             complement(35909..37492)
                     /gene="yegH"
                     /locus_tag="ACHJWA_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO4307854.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(37693..37842)
                     /locus_tag="ACHJWA_001212"
     CDS             complement(37693..37842)
                     /locus_tag="ACHJWA_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4307855.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            38151..39290
                     /gene="wza"
                     /locus_tag="ACHJWA_001213"
     CDS             38151..39290
                     /gene="wza"
                     /locus_tag="ACHJWA_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFO4307856.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            39296..39739
                     /gene="wzb"
                     /locus_tag="ACHJWA_001214"
     CDS             39296..39739
                     /gene="wzb"
                     /locus_tag="ACHJWA_001214"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFO4307857.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            39742..41904
                     /gene="wzc"
                     /locus_tag="ACHJWA_001215"
     CDS             39742..41904
                     /gene="wzc"
                     /locus_tag="ACHJWA_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFO4307858.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQSSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMEDQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQILKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            41997..42836
                     /gene="wcaA"
                     /locus_tag="ACHJWA_001216"
     CDS             41997..42836
                     /gene="wcaA"
                     /locus_tag="ACHJWA_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFO4307859.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            42839..43327
                     /gene="wcaB"
                     /locus_tag="ACHJWA_001217"
     CDS             42839..43327
                     /gene="wcaB"
                     /locus_tag="ACHJWA_001217"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFO4307860.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            43324..44541
                     /gene="wcaC"
                     /locus_tag="ACHJWA_001218"
     CDS             43324..44541
                     /gene="wcaC"
                     /locus_tag="ACHJWA_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFO4307861.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQALFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            44516..45733
                     /gene="wcaD"
                     /locus_tag="ACHJWA_001219"
     CDS             44516..45733
                     /gene="wcaD"
                     /locus_tag="ACHJWA_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFO4307862.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKITQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGICAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            45744..46490
                     /gene="wcaE"
                     /locus_tag="ACHJWA_001220"
     CDS             45744..46490
                     /gene="wcaE"
                     /locus_tag="ACHJWA_001220"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFO4307863.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGIAMAQGKFALFLNSGDIFHQDAAYF
                     VRKLKMQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKS"
     gene            46506..47054
                     /gene="wcaF"
                     /locus_tag="ACHJWA_001221"
     CDS             46506..47054
                     /gene="wcaF"
                     /locus_tag="ACHJWA_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFO4307864.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYVWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRKRVETE"
     gene            47083..48204
                     /gene="gmd"
                     /locus_tag="ACHJWA_001222"
     CDS             47083..48204
                     /gene="gmd"
                     /locus_tag="ACHJWA_001222"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFO4307865.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            48207..49172
                     /gene="fcl"
                     /locus_tag="ACHJWA_001223"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             48207..49172
                     /gene="fcl"
                     /locus_tag="ACHJWA_001223"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFO4307866.1"
                     /translation="MSRQRIFIAGHRGMVGSAIRRQLEQRGDVEQVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            49175..49654
                     /gene="gmm"
                     /locus_tag="ACHJWA_001224"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             49175..49654
                     /gene="gmm"
                     /locus_tag="ACHJWA_001224"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFO4307867.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            49651..50874
                     /gene="wcaI"
                     /locus_tag="ACHJWA_001225"
     CDS             49651..50874
                     /gene="wcaI"
                     /locus_tag="ACHJWA_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFO4307868.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            50877..52313
                     /gene="cpsB"
                     /locus_tag="ACHJWA_001226"
     CDS             50877..52313
                     /gene="cpsB"
                     /locus_tag="ACHJWA_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFO4307869.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPENDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEDAFLACPEESVDYEVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            52506..53876
                     /gene="cpsG"
                     /locus_tag="ACHJWA_001227"
                     /gene_synonym="manB"
     CDS             52506..53876
                     /gene="cpsG"
                     /locus_tag="ACHJWA_001227"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFO4307870.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDARLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPSSGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            53931..55325
                     /gene="wcaJ"
                     /locus_tag="ACHJWA_001228"
     CDS             53931..55325
                     /gene="wcaJ"
                     /locus_tag="ACHJWA_001228"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFO4307871.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKLGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            55327..56805
                     /gene="wzxC"
                     /locus_tag="ACHJWA_001229"
     CDS             55327..56805
                     /gene="wzxC"
                     /locus_tag="ACHJWA_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFO4307872.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDLI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYVLGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            56985..58265
                     /gene="wcaK"
                     /locus_tag="ACHJWA_001230"
     CDS             56985..58265
                     /gene="wcaK"
                     /locus_tag="ACHJWA_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFO4307873.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQVYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGVMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLSEAVSRERQTGMQMVQSVLERIGEVK"
     gene            58262..59482
                     /gene="wcaL"
                     /locus_tag="ACHJWA_001231"
     CDS             58262..59482
                     /gene="wcaL"
                     /locus_tag="ACHJWA_001231"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFO4307874.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THVAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESW
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGVIHGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            59493..60887
                     /gene="wcaM"
                     /locus_tag="ACHJWA_001232"
     CDS             59493..60887
                     /gene="wcaM"
                     /locus_tag="ACHJWA_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFO4307875.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTI
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITQDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGNYLSIPQNF
                     RVNNIQLDNTHLAYKLRGLQISAGNAVSFVALTNIEMKRASLELHNKPQHLFLRNIKV
                     MQESSVGPALIMNFDMRKDVRGVFMAKEETLLSLANVHAVNEKGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            61045..62040
                     /locus_tag="ACHJWA_001233"
     CDS             61045..62040
                     /locus_tag="ACHJWA_001233"
                     /EC_number="5.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,
                     octacis-undecaprenol 4-epimerase"
                     /protein_id="HFO4307876.1"
                     /translation="MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQ
                     IGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            62283..63176
                     /gene="galF"
                     /locus_tag="ACHJWA_001234"
     CDS             62283..63176
                     /gene="galF"
                     /locus_tag="ACHJWA_001234"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFO4307877.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
BASE COUNT        15469 a        15984 c        16933 g        15051 t
ORIGIN      
        1 aaagcaatcg acatgatgtg aaaatgtacg tggtatcgac aaagcagatg ctgaacaacg
       61 cgcagcgcgg cggttacgcg gttccggcat tcaatattca caatctcgaa acgatgcaag
      121 tggtggtaga aaccgctgcc aacctgcatg cgccggtcat catcgccgga acgcctggca
      181 catttactca tgctggtaca gaaaatctgt tggcgctggt cagcgcgatg gcgaagcatt
      241 atcaccatcc actggcgatt catctcgacc atcacacgaa atttgacgat atcgctcaga
      301 aggttcgttc tggcgtgcgc tcagtcatga ttgacgcctc gcatttgcct tttgcgcaaa
      361 atatttcacg ggtcaaagag gtggtggatt tttgccatcg ctttgatgtc agcgtcgaag
      421 cggagctggg gcaacttggc ggccaggaag atgatgtgca agtcaatgaa gccgatgctt
      481 tttataccaa ccccgctcag gcgcgtgaat ttgccgaggc aaccggaatt gattccctgg
      541 cggtcgccat cggcacggct catggtatgt atgccagcgc accggcgctt gatttttctc
      601 gactggagaa cattcgccag tgggtgaact taccgctggt gctgcatggc gcgtcagggt
      661 tatcgactaa ggatattcag caaaccatca aactggggat atgcaaaatc aacgttgcaa
      721 cggagctgaa aaatgccttc tcgcaggcgt taaaaaatta cctgaccgag caccccgaag
      781 cgaccgatcc ccgggattat ttgcagtcgg ctaaatccgc aatgcgcgat gtggtgagca
      841 aagtgattgc cgattgtggc tgcgagggca gggcataacg cacctgccat ttaacaagga
      901 aaaaacatga aaacgttaat tgcccggcat aaagctggtg aacatatcgg catatgttca
      961 gtctgttctg cccatccgtt ggttatcgaa gcggcgctgg catttgatcg caacagcacg
     1021 cgcaaagtgc tgattgaagc aacgtcaaac caggtcaatc aatttggcgg ttataccgga
     1081 atgacaccgg cagactttcg cgaatttgtt tttgcgattg ccgataaagt cggatttgca
     1141 cgcgagcgta ttattctcgg cggtgaccat ctggggccaa actgctggca gcaagaaaat
     1201 gtggatgcgg cgatggaaaa atccgtcgag ctggtaaagg catatgttcg tgctggcttc
     1261 agtaaaattc atcttgatgc gtcaatgtcc tgcgcggggg atcccatacc gttagcacca
     1321 gaaacggttg cggaacgagc tgctgtgctt tgctttgctg cggaaagtgt ggcgacagat
     1381 tgccagcgtg agcaactgag ctatgtcatt ggcaccgaag ttccggttcc gggcggtgag
     1441 gccagcgcca ttcagtcagt acacatcacc catgttgaag atgccgccaa tactttacgt
     1501 acgcatcaaa aggcctttat tgcccgtggg ctgacagagg cattaacacg cgtgattgcc
     1561 atcgtggtgc agccgggtgt ggaatttgat cacagcaata ttatccatta tcagccgcag
     1621 gaagcgcagg cgctggcgca atggatagaa aatacccgaa tggtttatga agcacattct
     1681 accgattacc agacccggac ggcttattgg gaattagtcc gcgatcactt tgcaatattg
     1741 aaagtcggtc ccgcattaac ctttgcttta cgtgaggcga tatttgcgct ggcgcaaatt
     1801 gagcaggaac ttatcgcccc cgaaaatcgc agcggttgcc tggcggtaat tgaagaagtg
     1861 atgctcgacg aaccgcaata ctggaaaaaa tattatcgca cgggttttaa cgattcatta
     1921 ctggatattc gttacagcct gtcggatcgt attcgttatt actggccgca tagtcggatt
     1981 aaaaatagcg ttgaaacgat gatggtgaat ctgcaaggcg tggacatccc actgggcatg
     2041 attagtcagt atcttcccaa acaatttgaa cgcattcagt ccggggaatt atcagcaata
     2101 ccgcatcagc tgattatgga taaaatttat gatgttttgc gcgcctatcg ctacggctgt
     2161 gcggaataag gacggtatat gactaacctg tttgttcgta gcggaatttc ttttgtcgat
     2221 cgtagcgaag ttttaaccca tatcggtaat gagatgctcg ccaaaggtgt ggttcatgat
     2281 acctggccac aggcattaat tgccagagaa gcagaattcc ctaccgggat aatgcttgag
     2341 cagcacgcca ttgcaatacc gcattgtgag gcgattcatg ctaagtcgtc agccatttat
     2401 ctgttaaggc caacaaataa agttcatttt cagcaagcgg atgatgataa tgacgtggcg
     2461 gtatcgttgg ttattgcgtt gattgtggaa aatccgcagc agcaattgaa acttttacgc
     2521 tgtttatttg gcaagttaca acagcccgat atcgttgaga cactaatcac tcttcctgaa
     2581 actcagttaa aggaatactt cacaaagtat gttttagatt cagacgaata aatccctctg
     2641 taacaataaa aaggactatt tatgaaacgc aagattattg tcgcttgcgg aggcgaggtt
     2701 gcgacctcta cgatggcggc ggaagaaatt aaagagttgt gtcagagtca taatattcct
     2761 gttgaattaa tccagtgtcg ggttaatgaa atagaaacct atatggatgg cgtgcatttg
     2821 atatgcacca ctgccagggt ggatcgtagt tttggcgata ttccgttagt tcacggcatg
     2881 ccttttgttt ctggtgtcgg tatcgaagca ttacaaaata aaattctgac tatcttacag
     2941 gggtgaccta tgttttcaga agtcatgcgt tatattctcg atctcggccc tacggtgatg
     3001 ttgccgattg tcatcattat tttttctaaa attttaggca tgaaggcagg cgattgcttt
     3061 aaagcgggtc tgcatatcgg gattggcttt gttggcattg gccttgtgat tggcttaatg
     3121 ctggattcca ttggcccggc ggcgaaagcg atggcggaaa atttcgacct gaatctgcat
     3181 gtggtcgatg tcggctggcc gggctcttca ccaatgacct gggcgtcgca aattgcgctg
     3241 gtggcgattc cgattgcgat tctggttaac gtggcgatgt tactgacccg tatgacgcgg
     3301 gtggtaaatg ttgatatctg gaatatctgg catatgacct tcaccggcgc gttgctgcat
     3361 ctggcaaccg gttcatggat gatagggatg gcaggtgtgg taattcacgc ggcgtttgtt
     3421 tataagctcg gcgactggtt tgcccgcgat acccgaaatt tctttgagct ggaaggcatt
     3481 gctattccgc acggtacgtc ggcgtatatg gggccgattg cggtgctggt cgatgctatc
     3541 atcgagaaaa tcccaggcgt taaccgtatt aaatttagcg ccgacgatat tcagcgcaaa
     3601 tttggcccgt ttggcgagcc tgtcaccgtg ggttttgtga tggggctgat tatcggcatc
     3661 ctcgcgggtt acgatgtcaa aggtgtattg cagctggcgg taaaaacggc ggcggtgatg
     3721 ctgctaatgc cacgggtgat taaacccatc atggatggtt taacgcccat cgctaagcag
     3781 gcccgtagtc gtttacaggc gaagttcggc ggtcaggagt tcctgattgg cctggatccc
     3841 gcattactgc tggggcatac ggcggtggta tcggcaagcc tgatttttat tccgctcacc
     3901 attttaattg ctgtttgtgt tcctggtaat caggtactgc cgtttggcga tcttgccact
     3961 atcggcttct tcgtggcgat ggcggtcgcg gtgcatcgtg gaaatctgtt ccgcacctta
     4021 atctcgggtg tcatcattat gagcatcacc ctgtggatcg cgacgcaaac tattggtttg
     4081 cacacccaac tggcggctaa tgctggggcg ttaaaagccg ggggtatggt ggcttcaatg
     4141 gatcagggcg gttctcccat tacctggtta ctgattcagg ttttctcccc gcaaaatatt
     4201 cccggtttca ttattatcgg cgcaatttat cttaccggta ttttcatgac ctggcgtaga
     4261 gcgcgtggct ttattaaaca agagaaagtc gttctcgcag aataattttt acctgagggg
     4321 gaggttatcc cccttaacaa ttaggagttt ttatgaaatc agtggtgaat gatactgatg
     4381 gtatcgtgcg cgttgcagaa agcgtcattc ctgaaattaa acatcaggat gaggtgcggg
     4441 taaaaattgc cagctcgggc ttatgtggtt ccgatttacc caggatattt aaaaatggtg
     4501 cacattatta tccaataacg ttaggccatg aatttagcgg ctatattgat gcggtaggat
     4561 ccggtgttga tgatttacac cctggcgatg cggttgcctg tgtgccgtta ttaccctgtt
     4621 ttacttgtcc agagtgtttg aaagggtttt attcccagtg cgcaaaatat gattttattg
     4681 gctcacggcg tgatggtgga tttgccgaat atattgtcgt taagcgaaaa aatgtctttg
     4741 ctctacccac ggatatgcct attgaggatg gggcttttat tgagccgatt accgttggcc
     4801 tgcatgcttt tcatttagcg caaggttgtg agaataaaaa cgttattatt attggtgccg
     4861 gaaccattgg cctgctggct attcagtgcg ctgtcgcgct gggagcaaag agtgtgacgg
     4921 ctatcgacat tagctcagaa aaactggcac tggcaaaatc tttcggtgcg atgcaaacat
     4981 ttaacagtag cgaaatgagc gcgccgcaaa tgcagagcgt tttacgcgaa ctgcgcttta
     5041 atcagcttat cctcgagacg gctggcgtac cgcaaactgt cgaactggcg gtagagattg
     5101 ccggtcctca tgcccaactg gcactggtgg gcacgttgca tcaggatctg catttaacat
     5161 cggcaacgtt tggcaaaata ttgcgtaaag agctgacggt tatcggcagt tggatgaact
     5221 actccagccc ttggccgggg caggagtggg aaacggcgag ccggttgctg acagaacgta
     5281 agttaagcct ggagccatta atcgctcacc gtggaagctt tgaaagcttc gcccaggcgg
     5341 tgcgtgacat cgctcgtaat gctatgccgg gcaaagtgtt gctcattccc tgaaaccgcg
     5401 ggccagcgtg atgctggccc ggtattgtgc aaaacagatc attcaccaat ggtccccctt
     5461 cgtttacact agccacaatt gaatatgggt aaatgacgat gaattcattc gagcgaagga
     5521 ataagatcat ccaattagtg aatgaacagg gaaccgtgct tgttcaggat ctggcgggag
     5581 tatttgctgc ctcggaagcg acaatccgtg ccgatttgcg ctttctcgaa caaaaaggcg
     5641 tggttacgcg ctttcatggc ggtgcggcga aaataatgtc tggtaatagt gaaaccgaga
     5701 cccaggaagt cgggtttaaa gagcgatttc agcttgccag cgcgccaaaa aacagaatag
     5761 cgcaggcggc agtcaaaatg atccacgaag ggatgaccgt tattctcgac agcggaagta
     5821 cgacaatgct tatcgccgaa ggattaatga ccgccaaaaa tatcacggtg attaccaaca
     5881 gtctcccggc agcgtttgcc ctttccgaaa ataaagacat tactctggtc gtctgtggtg
     5941 gcacggtccg ccataaaacg cgctcgatgc atggttccat tgccgagcgt tcattgcagg
     6001 atattaatgc cgatttaatg tttgtcggtg ctgatggtat tgatgcggtt aatggtatta
     6061 cgacctttaa tgaaggttat tcgattagcg gggcgatggt caccgccgca aataaagtga
     6121 ttgccgttct cgattcatcg aaatttaatc gtcgcggttt taatcaggta ctgccaatcg
     6181 aaaaaattga cattattatt accgatgatg ccgtgtcaga tgtggataaa ctggcattgc
     6241 agaagacacg ggtaaaatta attacggtgt agtattgttc tgtatgccgg ataaggtgtt
     6301 tacaccgcat ccggcattac ggattctccc tggttcaaga gagaatagtt tgattaacgc
     6361 aataatggac aatctggtgg taatcgacaa cgcaacgccg ccggaagtat ttcaatatgg
     6421 aagttttgcc cactcaacgg ttcgccatca agattaaaag tgatttcgtg tggggcttgt
     6481 atatcaaacc acgacgaagc gccttcgata atattcgggt tatcttcgtc agattttaag
     6541 gttgatacga gagccggaag aatttcatcg ccggtaaaaa tgcgcagttg cagcaagcca
     6601 tcgttaatta acgcgttcgg gcacaattgt tgaccgccac cggcctgacg cccgttacca
     6661 ataccaatga ccagggcgtc accttgccag tgaaagtttt caccgcggat ttcacaacgg
     6721 tccggttgca gagtatccat gcgcattaag ccatgaatga tgtaagagac gccacccagc
     6781 gcggctttta atttttccgg cgtttctgtg gtaatacgcg tcccaaatcc gcctgtcgcc
     6841 atattaataa aacaggtttg tttgttgacc tgcgccatat ctatcgcaat ggcgttaccg
     6901 gcaattgcca gtttcagcgc cttatccagt gcctcaggaa tccctacact ggtggcaaaa
     6961 tcattggcgg ttcctaatgg caaaattccc agcgcgggta tgtcatcccc ctcacactga
     7021 atcaacgccg tagaaacttc attaatggtg ccatcgccac caccggcaat caccgttgcg
     7081 acgcccaact tccgggcctc ctctacatat cgtgcggcat cgcctttctc ccaggtgacc
     7141 cgcacatgga tcgtcattcc ttcctcacgc aacagcataa ttgcttcgcg caagggtaga
     7201 ttgtcagtac ttttgccatt aagaatcagt aagctggcgg gaaattctgc catgatcgta
     7261 tgtgccttta tgattggtct atagatagtg tagagcagaa aagaaaaggt ggagtcagaa
     7321 caggatgaaa gtcggaggat aaatagtcag ttcaagtatg gaggattcag tggttaccac
     7381 caggagcttg caatggggac agcgaatact aacggcagcc accgataaat ttcaaaaaag
     7441 agcatatacc taatattcaa ctaaacagtg gcttcttcaa tataatatat taaagcctcc
     7501 atggagttac ccggaagggc ctccatgtcc gtaattccta cttatgtagg aaatgtttta
     7561 cagaacattt attataatcc tattcaatta taataatcat gccattatta tatttaaaca
     7621 ctagagagtg tcgttggtat ttaatggggg aaggtgagat gaaaaagata gctgctatat
     7681 cattaattag tgtttttctt atgtctggtt gtgctgtgca taatgatgag acaagtatcg
     7741 gtaaatttgg tcttgcatat aaaagtaata ttcagcgtaa actcgataac caatactaca
     7801 ccgaagccga agcttcttta gccaggggca gaatatctgg tgcagaaaat atagtaaaaa
     7861 atgatgcagc ccatttctgt gttactcagg gcaaaaaaat gcagatagtt gacctgaaga
     7921 cagaaggtgc aggattacat ggcgtcgctc gtctgacatt caaatgtgga gagtgagaat
     7981 attttttggt aagcgtcaaa catgcgcgtt ctggttgtgc ttagccggaa cctgtgcgag
     8041 cacgatgccg ttacgtgaaa ggcatcgtgc tatgaaggga gattctatcg atgtggtcaa
     8101 tggaagacgg ttatcaggga tagggcttat gcataaaaaa taagcccgtg taagggagat
     8161 tacacaggct aaggaggtgg ttcctggtac agctagcatt ttatgggtta tgtttttcag
     8221 cgaaacggat gataacctta ataaatgcag ctgtatgtga tcggtttcta agaattttcc
     8281 atccgggaaa aataatcgaa attaatcact taccgtgggg gttacgcgta gtttccccgg
     8341 agaaattacg catcagcagc gcgtaattta gctcaagatc ctgcggcacc gggagccaca
     8401 cagtataacc atcgcctggt gctaccggca tagcttcgcc tttggcgttt tccatgtgct
     8461 caagggtaaa gttaatgttg ccttgcggcg tcatcagctc aaggctgtcg ccaacggaga
     8521 atttattttt caccgctacc gccgcgaggt cccccttgcg ctcaccggta aactcaccaa
     8581 caaactgctg gcggtcagaa actgaataac cgtattcgta gttctgataa tcgtcgtgag
     8641 tatgacgacg caggaaacct tcggtatagc cacgatgcgc cagaccttcc agagtttcca
     8701 gcaggctggt atcgaacggt ttgcccgcag cggcatcatc gatagcttta cgataaacct
     8761 gtgcggtgcg tgcgcaatag tagaaagatt tagtacggcc ttcgattttc agcgaatgca
     8821 cgcccatttt ggtcaggcgt tctacatggg cgatggcgcg cagatctttc gagttcatga
     8881 tgtaagtgcc gtgctcatct tcaaacgcgg tcatatactc gcccggacgc tgggcttctt
     8941 cgatcataaa cactttgtcg gttggcgcgc cgatacccag cgtcggctca acattttgca
     9001 ccggaatcgg ctcgtacttg tgtacgatgt tgccaacgtc atcttctttc ccttcctgga
     9061 cgttgtactc ccagcggcag gcgttggtgc aggtaccctg gttcggatcg cgcttgttga
     9121 tatagccaga gagcaggcag cgaccggagt aggccatgca cagtgcgccg tgaacgaaga
     9181 tctcgatctc catatccggc acctgattgc ggatctcttc aatctcttcc agcgacagct
     9241 cgcgagagag gatcacgcgg gtcagtccca tttgctgcca gaatttcacc gtcgcccagt
     9301 tcacggcgtt agcctgtacc gagagatgga tcggcatttc agggaagtgc tcacgcacca
     9361 gcataatcag ccctggatcg gacataatca gcgcatccgg ccccatttcc accaccggtt
     9421 tcaggtcacg gataaaggtt ttcagcttgg cgttgtgcgg tgcaatgttg accacgacat
     9481 aaaacttttt ccccagcgcg tgggcttcat tgatgccgag ctgaaggttt tcgtggttga
     9541 attcgttgtt gcgcacacgc agggagtaac gcggctggcc cgcataaaca gcatctgcgc
     9601 cataagcgaa agcgtaacgc atatttttca gcgttcccgc cggggaaagg agttccggtt
     9661 taaacataat tctctcgttc tgatgacagg tcagatccgc ttcacctgat gaagcggtta
     9721 ggggagtgcc cccactttaa gggcgggcat tgtagcgctg taggggggat ctggtaaagc
     9781 gccggacgcc aacaacccac gcccggcatc gtaattacgt attgtctttc accgtctggc
     9841 ttgtgccatt ggctttaacg gaggcaattc cggtttcacg agattgcgcg gtggcataca
     9901 tctggctgga gccgataatt tgatgattgg cggctttcag attgaaatag aatttgccgt
     9961 tgcttgccgt ttttttctca tagcgttctt cttgcgggct gttgctacgc accgacgcga
    10021 tgcccttttc cgcagaggct tttgaggtat acagctcgct ggtgaggatc gtctcaccat
    10081 tgcccgcttt tagcacaaac cggaactgac tatcactgct cttactgagt tcaaaccaac
    10141 cagccataat gactccttga ttgaaattgc gtatcgaaaa cagcatctaa aggtgctgtt
    10201 taccgattaa agtatgagcg attttctgat gcctgccagg gcggagagat aatgtcagtt
    10261 tgctgaccat gcgtgggata atgttgccgg atctggtctg aatgccatct ccggctaaca
    10321 aagtaatgtc gctaaaacta aacgatgcgg caggcgtcgg cttcccagcg gtaaccgacg
    10381 ccgtaaacgg cgcggataaa tgactgttcg gcgtcgagag attccagctt gcggcgcagg
    10441 tttttaatgt ggctgtcgat ggtacggtcg gttactacgc ggtagtcgtc ataaagatga
    10501 ttgagcaatt gctcgcggga gaacactttt cctggttcat gagagagcgt tttcagcaga
    10561 cgaaattccg caggcgtcag gtcaagcatt ttaccgcgcc atgaagcctg aaaacgacct
    10621 tcgtcgataa tcaacgggct ttcagcatcc tgttgctgca actcacgctg cggtttgcaa
    10681 cggcgcaaaa tggttttgac acgcgctacc acttcccgtg ggctgtacgg cttgcaaatg
    10741 tagtcatctg cgccaatctc cagccctagc aggcggtcga tctcttcgat ttttgctgtc
    10801 accatcacga taggaatgtc agaaaaacga cgaatttccc ggcacagcgt caggccatcg
    10861 gtgccaggga gcatcagatc caacaggatc agatccggcg gcgtctggcg cacgtacgac
    10921 agtacctgat cgccgtggct gataagcgtc ggcgcatagc tcgcagcacg cagataatca
    10981 atgagcaact gtcccagctt cggttcatct tccacgatca aaatacgcgg tgtgttttcg
    11041 tcgattggta actcggtcat acttctctct gtaaatcccg ttccagcggt aactctactg
    11101 taatgcttac cccgccaaaa ggcgaatggg cggcaataat gcggccatta tgtgcttcaa
    11161 caatgttcag gcaaatcgcc agccccagcc cggaaccgcc gctggcgcgg ttgcgggaac
    11221 cttcggtgcg ataaaaacgt tcaaacaatt tttgtagctg atcgtcactg acacctggcg
    11281 cactgtcggc aaaggtcagg cgcaccgttt tgtcgtgctg ctcggcagag attttcaggc
    11341 tgccgccgct gtcagtgtag cgcaggctgt tttccagtaa gttattgaat aactgcatta
    11401 aacggtcgcg atcgccaaat acggtgatac tgtctggcag ggaaaattgc agtttcaggc
    11461 cacggctggc aaatcgttcg cgaaatgcgc cgcctgccac ttccagcagt gggatcaaat
    11521 ctaccggtgc tttttgatag gcgagagcgc cttcatcaga catcgacaac tgatgaagat
    11581 cgtcaaccag tttggtcagt gtaccgacct ccgcctgtaa agacgccacc gtctccggcg
    11641 tgaatttgcg cacgccatcc tgaatggctt ccagttcacc gcgcagcacc gctaatggcg
    11701 tacgcagttc gtgagaaata tcggccataa aatcgcgccg catttgctgg tttttctcca
    11761 gcgtgctggc gagctggttg aagtcttgcg ccagtttgcc cagttcatct tcactggtgg
    11821 gcgttacgcg ggtagtgaaa tcgcccgccg ccagtttgtg cgtgccatcg acaagtcgtt
    11881 ttaccggtgc cagcaaaccg cgcgccagca gaaaagtagc gagtgccgcg agtaacgttg
    11941 ccagggcgac aatcagccag ctggtttgcc gctgttgttt atcgaaattg atatcagtat
    12001 tgcgggtcag ccgctcaaca ggggaggcaa tcaccgcgcc cacttccgca ccgttgacca
    12061 gaatgggtcg ccgtgtaccg tcaggtggaa tcggcgctcg cggaccaacc agcaccttgt
    12121 tgttttgatc aaccacccag aactgagtac gccagccgtg tggtggcata cccgggccgg
    12181 gtttatcttc cgaattatcg tgttcaaatg aacgcaggat ctgaaagaca aagcgatcat
    12241 tgttgcgcag gaagcgccag ttgccatgct gcgcatactg ctcgccaagc gcatcactta
    12301 acagttgtaa tcgctgttca ttaccatgct tgatgtaatc aataaagcca cgctcaaaac
    12361 tgatacgcac cgcccagtgc atactgatca gcaagacaat gcaggtggcg aaaatcgcca
    12421 gaaacagttt gccggtaata ccaggtcgcc agaacttcat tgcgcgctcc tttttcgccg
    12481 cgaaatagct acattttgat gcgtatcgtt cggcactctg gcaaagatga acgccggaag
    12541 ggcgatgata aacgccatgc taagccaggt gtacataaag acggtttgtg tggtgctgct
    12601 gtcgacgctg acatgctgcg aaccaaaaag tcccagcaac agcccggcga tggtgacgcc
    12661 gatactcatc gacaattgca taatcatcga cagcagactg ttgccgctgc tcgccagatt
    12721 gtccgggaga tctttcagcg tcagggtgtt catggaggag aaacgggtcg agttgaccat
    12781 cccttgtaaa aacaggacga acggcaaaac gtagtaccag cccaacagag cggtagtcat
    12841 aaacaacagg gtgaccagcg acagacccag cgtggtcgct accagtaccc gacgataacc
    12901 aaagcgattc accacctgta caacaatccg cttcatcccc atgctgccaa gcaccatcgg
    12961 gatcatcatc agtccggcat gaaacggtga gaagccaagg ccaatttgca ggaaaaccgg
    13021 cgtcataaag ggcaacatgc cgctaccgat acgtccggca aagctccccg ccaggcccag
    13081 cgaaaaggta cgagtgcgga acagtttcag actgaaaaga gcgcggttgt tatttctggc
    13141 gtgcagcaga taaagcacca gtgccaccac accaactgcg accaggccag taatcgccag
    13201 cggcgataaa cctgtacctt tactgccgtc cagcgccagg gttaataccg ccatgccaac
    13261 cgccagcaat aaaaagccgg aaagatcaaa gcgccgcgtc tgcatggtgt agttcggcat
    13321 taacatcaat gtggcgatcg caccgataat ccccaccgga atgttgatca aaaagatcca
    13381 gtgccacgac gcgtactcca ccagcagacc gccgagcgcc ggaccgagca gcggaccgac
    13441 ctgaccgggt aacgtgacaa aggtcatcgc cgccatatat tgctcgcgcg gtacgatttt
    13501 catcaccgtc aacctgccga ccggcaccat catcgcaccg ccaacgccct gtaacgcgcg
    13561 tgccagcaac agttcgttca gcgtgccgga aagcgcgcaa aacagtgaac cgagagtaaa
    13621 cagcacgata gcggtaaaga aaatattgcg cacgccgact ttgtccgcca gccagccgct
    13681 ggcgggcagc atcaccgcca cggtcagcac ataagagaca atgaccatgt gcatatgcaa
    13741 cggactttcc ccgaggcttt gcgccattga gggaagggcg gtgtttacga tggtggtgtc
    13801 cagcgactgc ataaagaagc cgaaagccac aatccacaat tgccagcggg tgctgtcggg
    13861 aagatctgtc atttactcgg ttaccgtttg tttagattta cgcgaaaaac gcagccgcag
    13921 acggtcgaaa aagagatata ccaccggcgt ggtatacagc gtcaggagct gactcatcac
    13981 cagaccgccg acaatggtga tccccaaggg ttgccgcagc tccgagccgt caccgcccga
    14041 caacaccagc ggcagcgcgc caaacagcgc cgccagggta gtcatcataa tcgggcgaaa
    14101 acgcagcaga caggcctgga aaatagcttc ctgcggcgtc aggttaccgt gccgttgggc
    14161 ttcaagcgca aaatcgacca tcataatggc gtttttcttc acaatgccga ttaatagcat
    14221 gatccctatc agggcgatta ggctgaacgg ggcattgaac agctccagcg ccagcagcgc
    14281 tccaacgccc gccgagggca gggtggagag aatcgtcagc ggatgtacgt aactctcgta
    14341 aaggataccc agcacgatat acaccgtggc gatggcggcg ataatcagga tcacctgtga
    14401 gttcatcgtc tcctggaaca cctgcgccgt gccggcaaaa ctgccgcgca ccgtcgaagg
    14461 cacaccaagc tgggtcattg cgcgatcgat cgccgcactg gcgtccgaga gcgattttcc
    14521 ggtcggcagg ttgaacgaga tggtcgaggc tgccgataat ccctgatgat tcaccgatag
    14581 tggggcattc gccggttgcc atttagcgaa atacgacagc gggatcgctt ttccttcgtt
    14641 attgattacg aacatttttt ccagcgcgct gatgtcctgg gtatagcgcg gatccacttc
    14701 catcaccact ttatactggt tcatcggctg gtaaatggtc gagatttgcc gctgaccgaa
    14761 ggcgttattt aacaggctgt tggcggcctg tacgtcgatt cccagccgtg ccatggtgtc
    14821 gcgatcgtaa accagattca tctccgcgcc gttatcctgc tgatcggagt tcacgtccgc
    14881 cagttccggc aatgtcgcca gttttttgcg gattttcggc tcccattcgc gcagtgccgc
    14941 caggtcgtcg gataacaacg tgtactggta gctggcgttc gactgacgtc cgccaacgcg
    15001 aatatcctgt accgccatca ggaacagatt cgcccccggt tctttcgcca gcttcacgcg
    15061 tagacggtca ataatttgct gcgctgtttc gctgcgttcg tcgcgtggct tgagggtgat
    15121 aaacatcatc ccgctgttca ctcgcgaacc gccggtaaag ccggtgacat tatccactgc
    15181 cggatcgtca cggataattt tcatgaaatc ctgcaactta ccgcgcatcg cctgaaacga
    15241 aatactctga tccgcctgta tcccgcccat caacacgcca gtgtcctgct ccgggaagaa
    15301 ggttttcgga atcgagatat atagccagat attcaaagca atggtgccaa gcagcaccat
    15361 gcccaccaga cgggtatgat tgagcaccca tttcagtgac ttaccatagc cttgttgcag
    15421 ggctaccaac atgcgaccaa aaccacgcag tcgcttttgc tcgcgcggct tgctggcttt
    15481 cagcatccag ccacacatca ttggcgttaa tgtcagagaa accagcaacg aaataccaat
    15541 ggcgacagaa agcgtcacgg caaactcgcg taacagtcgg cccggcaatc cgcccatcaa
    15601 cagcagcggc aggaacaccg ccaccagcga cagactcatc gacagcaccg taaaaccgac
    15661 ttcgcgagta ccttgcagtg cggcttgcaa cggtttcatt cccgcttcca gatgacgtgc
    15721 aatgttttcc agcaccacga tggcgtcatc caccacgaaa ccagtagcga tggtgagcgc
    15781 cattaacgaa aggttattga gactgaatcc gcacaggtac atcgccgcaa acgtaccaat
    15841 cagcgaaacc ggcaccgcaa cggcgggaat aatagtggcg cgacccgagc gcaggaataa
    15901 aaagaccacc agaatcacca gcgccaccga gataatcagc gtttgctcga cttcttccag
    15961 cgaggcgcga atggtggggg agcgatcctg ggcaatttgc agatcaatcg ccgccggaat
    16021 ggtttcctgc aactccggta attttgcccg gatgctgtct accgtctgga taatattggc
    16081 ttccggcaat ttgcggatca tcagtaaaat agccggtttg gcgttggtca tcccggcgtt
    16141 gcgcacatcc tgcactgagt cggtcaccgt cgccacatcg cccagacgaa ccgcgccgcc
    16201 gttgttgtag tgaataatca gcggctgata ttcagcggcg gttttcagtt catcgttggt
    16261 ctggatctgc cagcggtgag tgccatcttc cagcgccccc tgcggtttac gcacgttggc
    16321 attgctgatg gcagtgcgta cgtcgtccag cgacacgccc tgattaaaca gcgcctgcgg
    16381 gttcagcccg acgcgcacgg cgggcagcga gctgccgccg acatcgacat caccgacacc
    16441 gtcgatttgc gaaatcgtcg gagccagctg cgtcgaggcg aaatcgtaca gttcaccctg
    16501 cgaataagta tcggaggtta gcgtgaggat cataattggc gcatccgacg ggttcgcttt
    16561 gcgataagtc gggcggctgg gcatcccact gggcagcaga ctttgtgcgg cgttgatcgc
    16621 cgcctgcaca tcacgcgccg caccgttgat atcccggtca aaatcaaact gcaaaataat
    16681 acgcgtgctg ccgagcgaac tgctggaggt catttcactg accccggcaa tgcgcccaag
    16741 tgagcgctcc agcggcgtgg caacggaaga cgccattgtc tccggcgacg caccgggcag
    16801 cgaggcgctg accataatca ccggaaaatc gacctgcggc agcggggcga ccggcagcat
    16861 acggaaaccc agtatgccgc acagggtaat ggcgaccgac agtaaaatcg tcgccaccgg
    16921 gcggtaaatg aagagggcaa aaaacttcac ttacgcctcc tcttcatggc gagcaaagcg
    16981 acttttggtc cacaatgcca ggcggtcaaa cagcagataa atcaccggcg tggtgaacag
    17041 cgtcagcacc tggctgacaa tcaggccgcc gaccatgccg atacctaacg gacgacgtag
    17101 ttccgcgccg accccggtac tcaacatcag cggcagcgcg ccaagcagag ccgccagggt
    17161 ggtcatcagg atcggacgaa aacgcaacag acaagcctgg tagattgcat cgcgcggcga
    17221 catgccttgc tcgcgctcag cagccagcgc gaagtcgatc atcatgatgg cgttcttctt
    17281 cacgataccg atcagcaaaa taatgccgat aatcgcaatc acatccagtt cgctaccggc
    17341 aatcattaac gccagcaggg cgccaacacc tgccgtgggt agcgtcgaga gaatggtgat
    17401 cgggtgaata aagctctcgt acagaatgcc gagcacgata tacatcgcca ccaccgccgc
    17461 gacaatcagc cagacagtgc tgcccagcgc cgactggaag gcgagggtgc tgccctggaa
    17521 ttgcgtggtg atatccaccg gcagattcag cgtcttctcg gtgtccataa ttgcctgcac
    17581 cgcatcgccc agcgaatagt tatccggcac gttaaaggag atggtcgtta ccgggaactg
    17641 atccagatgg ttgatggaaa gcggcgcaaa acgctgctca attttggcaa ttgagcttag
    17701 cggcaccacg ccgccgtcgc tgctggtcag gcgaatggta tccagcgccg cgaggcctgg
    17761 ggtattttcg gtattgtgct ccagcactac acgatactgg ttagcctgag tataaatagt
    17821 ggaaatcagt cgctgaccaa acgcgttgta cagggcgtta tcgacatccg ccatgctgat
    17881 ccccagacgg ctggcgctgt cgcgatcaac attgacatac gccaccagcc ctttgtcctg
    17941 ccagtcgctg gagacatcag aaatctgtgg cagctgctgg agtttttcca tcaactgtgg
    18001 cacccaggta ctgagcgcat ccagtgacgt ggcctgcaag gtaaactggt actgggtgcg
    18061 gctgacctga gtatcgatgg tcaggtcctg cgttggttgc aggaagagat cgacgcccgg
    18121 cactttatcc accgccgttt gcagacgggc gatgactttt tgcacccgat catcacgttc
    18181 atccaacggt ttgaggttga tctgcaaacg cgcactgttc agcgacgggt tagtgccatc
    18241 aacgccaaca aatgaggtca gactttgcac tgccggatcc tgcaaaatca cgtccgcgac
    18301 ctggcgttgc cgctgggcca tattggcaaa ggagctggat tgcggtgcct gcaaagtgcc
    18361 ctgaataatg ccgttatcct gtaccgggaa gaaacctttc ggaatgaaca cccacaatag
    18421 aacgctaagc agcagcgtgc tgagcgccac gcttaaggtc agccacggat gattcagcac
    18481 tttcgccagt ccacgaccat aggcggcgat tatcctgtcg aacatttttt ccgaggcacg
    18541 ggagaagcgg ttctgtttac gcaacgactc ctggctgagc atccgtgcgc acatcatcgg
    18601 tgtcagggtt agcgacacca cggctgagat taaaatcgct accgccaggg taatagcaaa
    18661 ttcgcggaac agtcgcccga cgatatcgcc cataaacagc agtgggatca acaccgcaat
    18721 cagcgagaag gtcagcgaga taatggtaaa gccgatttca cctgcgcctt tgagcgccgc
    18781 cgccaacggt ttttcgcctt tttcgatata gcgggaaata ttttcgatca ccacgatggc
    18841 gtcatcgacc acgaacccgg tggcgatagt taacgccatc agcgtcaggt tattgattga
    18901 aaaatcgaga aacaccatga ccgcgaaggt gccgattaac gacagcggca ctgcaacgcc
    18961 gggaatgatg gtcgccggaa tattgcgcaa aaacaggtag ataatcatga ctaccagcgc
    19021 gatagccatc atcaattcaa actgagtatc atcgacggat gcgcggatat tggtggtacg
    19081 gtcagaaagt accgtcacct tcaccgattt cggcagactc tcggtgagct gtggcagcat
    19141 ctgccggatg ctgtcggcgg tggagataat gttagcaccg ggctggcgct gaacattcat
    19201 cacaatggcc tgttctttat tcgcccatgc gccgagccag ctgttttctg caccttgctc
    19261 tacagttgcg acatcgccca gacgaatcgg cgcgccgttc tggtaggcga tgattagctg
    19321 gcgatactct tcggcggatt gcatctggtc gttagcggaa agcgtaaccg cacgggaagg
    19381 gccgtcaagg ctacctttag ccgagttaac gttagcgccg gtaatggcgg tgcgcacggt
    19441 ttcgctggtc aggccgaggg cggcaatcgc ctgagcgtta agtttgacgc gaacagccgg
    19501 acgctggccg ccggaaagcg tcaccaggcc gacgccggaa atctgcgaga ttttctgcgc
    19561 gacgcgggtt tccaccatat cttccacttg cgtcatcggc atggctgttg aggtgacggc
    19621 gagcgtcatg atcggcggat ctgccgggtt cactttgctg taaaccggcg ggttaggcag
    19681 atcgctcggc aacaagttgg tcgcagcgtt aatcgcggcc tgcacttcct gctcggcgac
    19741 atccagcggt aatgttagct ggaactgtaa agtgataact gacgcaccgc cggaactttg
    19801 cgacgacatc tgtttcaggc cagacatctg cccgaactgg cgttcgagcg gcgcggtaac
    19861 ggcagaggtc atgacatccg ggctcgcacc tgagtagagc gtgaccacct gaatggtcgg
    19921 atagtccact tccggcagcg ccgaaacggg cagggcgcga taaccgataa tcccggcgag
    19981 taagatcgcc accatcagca gcgtggtggc cacaggacgc ataataaaca ggcgcgacgg
    20041 gccgcctgtg ctgctcgggg gtaacacttg catcaggagc gagctccttt tttcgcgtat
    20101 tcgcggctgg tggctttctc ttccggagtg gtggcgctct gggcttccac cacttccact
    20161 ttcgcccctt cggtcaggcg atcaatgccg tctgtcacca cgcgatcgcc cgcagaaata
    20221 cctgcgcgga tcaccacttt ctgactgtcc tgaatgcccg gcgtcaccag atgtttgctg
    20281 accttgtttt cgctattcag cacccagaca aaatggcctt cattgcccat ttgcagggcg
    20341 gctgttggga tcactacggc gttttgttcg gtgtcgacta acatgcgcgc gttaacaaac
    20401 tgattgggaa acagcgcatc atcctgatta ttaaagcgtg ctttcacttt aatcgtaccg
    20461 gtagtggcat cgatttggtt atcgagactt aacagcgtgc cttcacttaa tttcttcgag
    20521 ttggtgcgat cccaggcttc taccaccagc ggttttccgg ctttttgcgc ctgcacgacg
    20581 gtagcgatat cgctttccgg cagggtaaag agcaaatcga taggatgcgt ctgggtgatc
    20641 accacgatcc cggtggtatc accactggag atttggttac caacatcaac ctgcttgaga
    20701 ccaacgcgac catcgactgg tgcggtaata cggctccagt cgagttgcag ctgcgcgctg
    20761 gcgacgcttg cttcatcagc cttaatggtg ccttcggttt cactgaccag cgcctgttgg
    20821 gcatccagct cctggcggga gacgagattg gtttttgcca gttgctgata acgcgccaga
    20881 tcacggcggg cgttggtaag cgtggcttta tcttttgcca gttggccctg ggcttgtgct
    20941 aatgcaactt tgaactggct ggggtcaatt tctgccagta aatcgccagc tttgacctgc
    21001 tggccttcct ggaaatgtaa cgccatcagt tgaccgtcca cgcggctacg caccgtaaca
    21061 gtattagcag cggtaatggt gccaagcccg gtgaggtaac gcggaactgc ctgttctacg
    21121 gcggtcgccg cctgaaccgg ggctaatggg ccggaacgca taccacggcg accacccgct
    21181 ggcgattgct gcgcttgttt cgtcgcccct ggggctgcac tctgggagtc attgcggcct
    21241 tgccagaacc agaatgcggc gatggcggcg ataaccacca cgattacgat tacccaacgg
    21301 gatttataac tgcctttcat cgttaagagt ttctcttcct gaaacgtttc gcggaatgat
    21361 actagtgtag ccagccaata agggagaaaa atggaggaat tatgaaacac tatcgggaat
    21421 cgtctgaatg gcgggcacat ttgcgagcac gcatccagta ataacacagg aaactatttt
    21481 atctacgcgt tagcgataga ctgcttgcat ggcgaaagga ggtaagccga cgatttcagc
    21541 gggacgctga aacgggaaag ccccctcccg aggaaggggc cataaataag gaaagggtca
    21601 tgatgaagat actcatcatc gtggtgctct tagtgattag ctacccagca tactaagact
    21661 accagggcgg gggaaacccc gctctaccct cactcctgaa agtatgcctt cacgttcaga
    21721 atgtcaatcc gcaggctttg cagtctgcga tcctgccagc aaatattctt tgcgagacgt
    21781 tacgcaataa tcacagagga aactatttta tccgcaagtc agcgatagac tgaattcagg
    21841 gcgaaaggag gtaagccgac gatttcagcg ggacgctgaa acgggaaagc ccctcccgag
    21901 gaaggggcct taaataagga aagggtcatg atgaagtgga tcatcatcgt ggtgctctta
    21961 gtcataagct tcccggctta ctaagactac cagggcgggg gaaaccccgc tctaccctca
    22021 ctcctgaaag tatgccttca cgataagatt gtcaatctgc aggctttgca gtctgcgatc
    22081 ctgccagcaa atattctttg cgagacgtta cgcaataatc acactggaaa ctattttatc
    22141 cgcaagtcag cgatagactg catgcatggc gaaaggaggt aagccgacga tttcagcggg
    22201 atgctgaaac gggaaagccc ctcccgagga aggggcctta aataagaaaa gggttatgat
    22261 gaagcacgtc atcatactgg tgatactctt agtgattagc ttccaggctt actaagaaca
    22321 ccagggggag ggggaaacct ttccctaacc ctcactcctg aaattatgtc actccgccct
    22381 tagtgtcaat cgacggctct ccgttcttgc gaggcgaata gcaaaatttt ctgcgccgcc
    22441 gggcctaatt ctgaaagttt cttcgtttgc tcttatttca ttattgcaat cggtatgata
    22501 ccggacctca ataattctct aagaaaggat ttcttatgag cgaacaaatc acgtttgcca
    22561 ccagcgattt tgccagtaac ccggaaccac gttgcccctg cattttgctg ctggatgttt
    22621 ctggttcgat gaacggtaga cccattaatg aacttaacgc cggattggtt acctttcgcg
    22681 atgagctgct tgccgacccg ctggccttaa aaagagtgga acttggtatt gtgacgttcg
    22741 gcccggtgca tgtggaacaa ccctttacca gcgccgctaa ttttttcccg cccatcctgt
    22801 ttgcccaggg ggatacacca atgggtgccg ccattaccaa agccctggat atggtagagg
    22861 agcggaaacg tgagtatcgt gcaaatggta tttcgtatta tcgcccgtgg attttcctga
    22921 tcaccgatgg tgcaccaacc gatgagtggc aggctgccgc caacaaagtg tttcaggggg
    22981 aagaagataa gaagtttgcc ttcttttcca tcggtgttca gggggcggat attaagactc
    23041 tggcacaaat cagcgttcgt cagcctttgc cactgcaggg attacaattc cgtgaactgt
    23101 ttagctggtt atccagttcg ctgcgttcgg tttcccgctc cacgccggga acggaagttg
    23161 tgctggaagc gccaaaaggc tggacgtcag tgtgagctgg cgtctggttt atgcatccgc
    23221 tgttggaacg tcgcacatta gcgctgattt accttgtcag gatgcctgcc agatgcaggt
    23281 tgcctggcta aatgaccagc agccattact ggtgatgttc cttgctgatg gcgcaggcag
    23341 tgtctcgcag ggtggcgaag gcgcgatgct tgccatcaat gaagcgatgg cttatgtgtc
    23401 gcaaaaagtg cagcacggtg aatttggact caatgatatt ctcgccacgg atattgtgct
    23461 gaccgttcgt cagcgacttt ttgccgaggc ggaagctaaa gaattagcag ttcgcgattt
    23521 tgcctgcacg tttctgggat tgatttcatc ggctaacggc accttgatta tgcagattgg
    23581 cgatggcggc gtggtggtgg attttggtca cggtctgcaa ttaccgctta cgccgatggt
    23641 gggtgaatat gccaatatga cgcatttcat taccgatgaa gatgtcgttt ccagactgga
    23701 aactttcacc agcactgagc gtgcgcataa agttgcagca tttacggatg gtattcagcg
    23761 actggcgtta aatatgctgg ataattctcc gcatgtgcct ttctttaccc cgtttttcaa
    23821 tggactggcg gcagcaacgc aggaacaact cgatttatta cctgaattgt taaagcagtt
    23881 tttgtcgagc ccagcggtaa acgagcgtac cgatgatgat aaaacgttga cactggcgct
    23941 gtggttacca tgaaacccac tttatatact gctactggtg agtgcgttac gccaggccgt
    24001 gaactgggca aaggtggcga aggcgcggtt tatgatatca atgagtttgt cgatagcgtc
    24061 gccaagattt atcacacgcc gccacccgcc ttaaaacagg acaaacttgc ctttatggct
    24121 gcgacagctg acgcgcagtt gttgaattat gtcgcctggc cgcaggcaac gcttcacggt
    24181 ggacgaggcg gaaaagttat cggttttatg atgccaaaag tttctggtaa agaaccgatt
    24241 catatgatct atagcccggc acatcgtcgc cagagttatc ctcattgtgc gtgggatttt
    24301 ctactctatg ttgcgcgcaa tattgcttca tcttttgcta cggttcacga gcacgggcac
    24361 gtcgtggggg acgtaaacca gaacagcttt atggtaggtc gcgacagcaa agtggtgttg
    24421 atcgatagtg actcctttca gattaacgcc aatggcacac tgcatttatg cgaagtcggc
    24481 gtgtcgcatt ttacgccgcc agagctacaa accttgccat catttgtcgg ttttgaacgt
    24541 accgcgaatc acgataattt tggccttgcg ttgctgattt ttcacgtctt gtttggtggg
    24601 cggcatcctt attctggtgt gccgcttatc tctgatgcgg gtaatgcgct ggagacggat
    24661 attgcccatt tccgttatgc ctacgcgtca gacaaccagc gacgtggttt aaaaccgccg
    24721 ccacgatcga ttccgctgtc gatgttaccg ggcgatgttg aagccatgtt tcagcaggca
    24781 ttcacggaaa gtggcgtggc aaccgcgcgt ccgacggcaa aagcgtgggt agcggcactg
    24841 gatttactac gccaacagtt aaagaaatgt accgtttcgg caatgcatgt ttaccccggt
    24901 catttgactg actgcccgtg gtgtgcgctg gataatcaag gcgttatcta ttttattgat
    24961 ctcggcgaag aggtcattac caccagtggt gattttgtgc tggcgaaagt ctgggcgatg
    25021 gtgatggcgt cagtagcacc gccagcattg caattgccat tacccgatca tttccaaccg
    25081 actggcaggc cgcttccttt aggcctgtta cggcgcgaat acatcattct gattgagatc
    25141 gcactgtcag cgttatcgct gttgctttgc ggccttcagg cagaaccgcg ttatattatt
    25201 ttggttcctg tgctggcggc tatctggatt attggcagtc tgacaagcaa agtttataaa
    25261 gcagaaatcc agcaacgccg tgaggcattt aatcgcgcga aaatggacta tgaccattta
    25321 gtcagccaga tccaacagtt gggcgggctg gaaggtttta tcgccaaacg ggcgatgctc
    25381 gaaaaaatga aggacgaaat tctcgggtta ccggaagagg agaaacgcgc tctggcagca
    25441 cttcatgaca ctgcaaggga acggcagaag cagaagtttc tggagggatt ttttattgat
    25501 gctgcctcta ttcccggcgt tggccctgcg cgtaaagcgg cgttacggtc ttttggtatt
    25561 gaaacagccg cggatgttac ccgtcgtggg gttaagcaag ttaaagggtt tggtgatcat
    25621 ctgacccagg cggttatcga ctggaaagcg agctgtgaac gccgttttgt gttcaggccg
    25681 aacgaagcgg taacgcctgc agaaagacaa gcggtaatgg cgaaaatggc cgccaaacga
    25741 catcggctgg aatcggcgtt gactgtcggc gcgacagagt tgcagcgatt ccgccttcat
    25801 gctccagcac ggaccatgcc gttgatggaa ccgttacgtc aggcggcaga aaaactggct
    25861 caggcgcagg cagatttaag ccgctgctga gccatttttc aattaacgaa acaccacttc
    25921 cgcccagcgt gccagcccgg cggtgacgga gccaaagtca tcgccgcctg caatcggaat
    25981 gcccggcaac tgttctgcca gcgctttttt aatcagcgga gaacgggcgc ttccgccggt
    26041 caggtagata acgtccggtt tttcctgggc gttatccagc gccagttgca cctgttcctg
    26101 aatccgcgcc agtggctggc tgagggcgct ttccagccct tgctggctaa tcagcgtagc
    26161 cagttcatcg ctgataaacg gcagtgaggc gcgggtttcc gctacgcttg aaagcgcaat
    26221 tttgcactct tctgcgctac gcaccaggcg atagcttaaa cgctgacgcc agactttctg
    26281 tagcagggcc actttttccg gttcgcgggc atcgcgtacc agatcgttaa gcagacgacc
    26341 gttggcacta ctgtagaaat cactctgcgc aggtacgtcg ttgatggcaa ccgcattcca
    26401 ccacggcaga atcggcaggg cgatgccttt ttcggtttcg ccacccatac ccagcagtgg
    26461 catcaggttt ttaaacgcca gtgcgatatc cagatcgtta ccgccaatac ggcaaccact
    26521 gtgacccagc aggctggctt cacgatcgag acgcgaacgc cactgcggcc ccatcagcag
    26581 caatgaacag tcagtcgtac caccgccgat atccaccacc agtacccgtt tttcttcctg
    26641 taaggtggct tcgtaatcca gcccagccgc taccggctcg tactggaata ccacgtccct
    26701 gaatccggca cgcttcgccg cgcgttccag aatcccttgc gcctgggcgt ttgcttcatc
    26761 gccgcccagc ccctggaagt tgatcgggcg accaatcacc gcctgagtaa tcgcttctgg
    26821 cagctgcgct tgcgcctgct ggcgaatgtg caacatcatt gcgcagacca gatcctcaaa
    26881 cagcgctacc tgctgcggtt ttaagccgct ggcaccgagg aacgattttg gtgatttcac
    26941 aaaccacact tcttctggat catcaatgta ctgtgccagt gaggaaagac cgaactgcac
    27001 gcttttcgcc gtaacatcga tatcttcttc gcgattataa cgaatcgccc gacgcaacag
    27061 tgcctgcgtt tcatcgtcgt ctgccggaac atcatgatgg cggtacagcc attcgcttac
    27121 cgcttcacgc gttggcgcgc aaagcattga aggcagcagc gtgctgtcgt tttccatttt
    27181 tagcaagtgc ggtttaccgt cacgcatgac cgctactgaa cagtttgctg taccgtaatc
    27241 aaaaccaata aacacgaaat aatccccatg ccggtgaaga aggggcgtga ctttagcgaa
    27301 atgttgccgt cgcgacaacc ggaatatgaa agcaaagcgc agcgtctgaa taacgtttat
    27361 gctgaaagcg gatgaataag gagatgcgat gtataccctg aactggcagc cgccgtatga
    27421 ctggtcgtgg atgttgggat ttctcgccgc ccgtgcagtg agtggtgtgg aaacggtcgc
    27481 tgacgattat tatgcccgta gtctggcggt gggcgaatat cgcggcgtgg tgactgctat
    27541 tccggatata gcccgccata ctctgcacat aaatttaagt gcagatttag aacccgttgc
    27601 cgctgagtgt ctggcgaaaa tgagccgcct gcttgatctg caatgtaacc cgcagattgt
    27661 taacggtgcg ttgggcaagt taggcgcggc gcggcccgga ttgcgtttac ccggcagtgt
    27721 tgatgctttt gagcaaggcg tgcgggcgat tttaggccaa ctggtgagcg tggcgatggc
    27781 ggcaaaattg actgccaaag tggtacagct ttatggcgaa cggctggatg attttccgga
    27841 gtatatctgc ttccccacgc ctcagcggtt ggcagcagca gacccgcagg cattaaaagc
    27901 gttaggtatg ccgttgaaac gggcagaggc gctgattcat ctggcaaatg cggcgctgga
    27961 gggcacctta ccaatgacaa taccgggcga tgtggagcag gcgatgaaaa cgctgcaaac
    28021 ttttccgggt atcgggcgct ggacggcgaa ttattttgct ttgcgtggct ggcaggcgaa
    28081 agatgttttc ctgccggatg attatctgat taaacagcga tttccgggaa tgacaccggc
    28141 gcaaatccgc cgttatgccg agcgctggaa gccctggcgt tcttatgcgc tgttgcatat
    28201 ctggtatacg gaaggctggc aaccagacga agcatgacgt tggtcgccgg atgcggtgcg
    28261 agcgccttgc ggaatgagcg atttgtaggc ctgataagac gcgttagcgt cgcatcaggc
    28321 aatgggcaaa cgctccctca gttaatcgcg aaataactac tattcatcag caaatccagc
    28381 ggttgggcat cggcaatcac ctcaccataa atcagatcga cgccgatgcc agaaagcgta
    28441 tccatcacta agggtaaaac gactggcccg gcgatggttt tcatcccgag tcgctgagcg
    28501 tgcccctgaa taatcgtaat cagcatctca tccatcaaat taccctgcac gttggcgcat
    28561 aactcaccat caagtagcag gtaatctgcc atattcgctt tcagcgagtt gaagatttgc
    28621 agatcgcggc ccacctgact gaatacgatc cggcatcccc ccagccgcag tttttgcacg
    28681 ctttctgcgt gatcgaaaat cgcttctgcc ggaataatca gatgtaataa ccgtggtggt
    28741 agagggctat tttccagctg ctcaagcaga tcattcacca gcgtggcgct actcaaaccg
    28801 gcaacggaaa gggggagggc gatgcttatg cctttactgg caaccgcttt tgcggcctgc
    28861 tggagaaatt cgtggaatac ccggcggtca agagcatggc taagcgccgg atcgctgaag
    28921 ctacgacgaa atgtttgttc atcaataatc tcgccttcgc aactccagag cttaagtgaa
    28981 atcagccaca aattacgcgc ttccgggatc cgtggcgaag cgacaccgtg ggcgagcatc
    29041 atcaactgat tctctttaat catccgccac tgttcatcaa gcgacatcgc cgcccgttcg
    29101 ctatgtgcgg cagcttgctg cggttcgtaa accgtcaccc ggccccggcc accatttttg
    29161 gaggcataac aggcgatatc agcctgcgac atcacttctg ccgcctgatg attgttgtca
    29221 tcgatcaagg taatcccggc gctggcacct acccgatgta cccggccttc ccatataaag
    29281 tgatagtcat tcacagcact gataatacgt gtagcgataa aacgagcgct ttcaacattg
    29341 cagtctggca gcagcagacc aaattcatca ccgccgagcc gcgccagcac gtcactggag
    29401 cgcagcatac tcagcatcaa cgacgccagt tcgcgcagca aagcgtcgcc cgccgcatgc
    29461 ccggcgctgt cattcaccgc tttaaagcga tcaagatcga taaacaccag tgcatgtcgc
    29521 tgatgcgtac tgtttaccgt ttgcagcagg atacgcagtt gtttctcaaa actggcgcgg
    29581 ttggcgagat gcgtcagtgc atcatgggag gcgctgtagc tcagctggcg cagcattttg
    29641 cgtgattcgg taacgtcctg aatcaccagt accgagccta tattgctgcc gtccagagta
    29701 cttaacggcg taatactgta atgcacgtcg tagctgccgc cgctccgaca gtgcaacacc
    29761 acatcttgtt cgatcgcgga acgtgaggtg tcggcactgt aaatgttctc cattaatggt
    29821 ccgttgtcgc caaaagtaat atgcaacacc gtcaggagcg gaacacctaa cgcttcttcc
    29881 tgcgtccagc cgctcatctt ctccgccact ggattcataa aggtaatttt catcgccata
    29941 tcaatacaga ccacggcttc gccgatggag tccagcgtaa tgtgcaggcg ctctttttcc
    30001 tgaaacaatg cctcgttaag ctgtttaacc tcagtcatat ccatattaat gccgagcagg
    30061 cgttcgactt cgccttcttt attcagtacc cggttggcga gggcgcggat atggcggatg
    30121 ccatctttca cggcaatgcg aaattccagt ttaaagggcg agcgtgattg caacgaatca
    30181 cgaatcactt tttcggcgtg ttggcgatct tccggcagca cgcactcgta ccacacctgc
    30241 cagttcggtt tgatatgcgg aggaatttca tacagctcga acatccgctt atcccagcta
    30301 aaaatattcg gcttcagttc ccactcccag atgccaatcc cgcccgcttc gttagccagc
    30361 gtgatgcgtt ccatcagttg ctgattcacc tgttcggtgc gttttagctc gttaatgtct
    30421 tcaatctgag cgataaaata gagcggcgtg ccatccgtgt ggcgcaccag agagacggca
    30481 agcaacgccc agacaacatc gccattgcgg ttgtagtaac gtttttccat tgaataggtg
    30541 tttatttcac cgcttatcag cttttcaacc tgttggagat ctttattgag atcctccggc
    30601 caggtcagtt gctgaaaggt gagtccgcgc agctcttcct gactgtaacc gagaaactgg
    30661 cagagtgctt tgttggattg cagccattgt ccctcggtgc ccactaatgc catgccgatg
    30721 gcggaatatt ccatggcgtt ccgaaaacgg gtttcgcttt cggaaatgtg tttgcgttcc
    30781 gcacgaaagg catacatcac catcgtcatg atgttggcgg gcagcaggat cagcaaaaac
    30841 ggtagccacg gcatatggct catcaggtac gtacgcggcg tagcaagcag ggagggatcc
    30901 gcggccatca tcagcgacac catcatcacc gtggtaagga agatcaaaaa ggcttccatt
    30961 cgtggcaggc gcacggcgct ccacatcaac agcacaataa tgaaagtaaa aggccacggc
    31021 agatacagca tcgaaagcca gcttaacgtc agtgtgatgg ctaacgtgag cagcgactca
    31081 aaaagcaacc gtgggttgcg atggcgcagc agatagtgtg gtttaaataa caatcccagc
    31141 ggcaccagtg ccagagcgcc gatggattct gacagtaccc atatcaaaaa tgccctgatg
    31201 aggatcgtct ccgggcgtca gcaggacaac cagaacaccc cccaacagag gtggaacaat
    31261 ggcgctgccg agtgccagac gcagccagtc agccagattt tgcaaggggt tataccacgg
    31321 cagcaattta cgtagcagca ctgccccgac cacggcttca acaatattga tggtcgtgca
    31381 ggtcatgttc agcgagctgg tggaaaaaag caggatggat gcggcgatat ttcccagcga
    31441 gcagctcagc gcaatccctg gccacatgcg cccggcatgg cgataaaacg ccaccatcat
    31501 gatggacgtc gggaaccaca gtggggcgag ttgggtgcca aactgcgaaa gctcaagcga
    31561 gaaaagggtg aagataaacg agactaaacc taaactgacc aggtgaagca gcgggtgggg
    31621 cagggcaatt aatacatgct gtgattgttt gctcattacc gctttatccg caaggccagg
    31681 tcgccagtat tcgtttctgt gtttatgcta gtacaaaagt tttacatttt atatgcgaat
    31741 tgctcataaa gtgacgtaaa ggcggataat ttgcgcaact gcgtataaca atttttacct
    31801 tgtataaaac tgatcaacgt aatttgccac cgggaattct tcgtcaacaa ttaactctga
    31861 gcgcgcggca acgctattcg actggtatca ggcggatgaa atccctataa ttgccgcgtt
    31921 tggcgcttcg tcgccccctt cctaacatcc aggttaatca ggtcgctaaa tatatgactg
    31981 atcagtctca tcagtgcgtc attatcggta tcgctggcgc atcggcttcc ggcaagagtc
    32041 ttattgccag taccctttat cgtgaattac gtgagcaagt cggtgatgaa cacatcggcg
    32101 taattcccga agactgctat tacaaagatc aaagccatct gtcgatggaa gaacgcgtta
    32161 agaccaacta cgaccatccc agcgcgatgg atcacagcct gctgcttgag catttacaag
    32221 cgttgaaacg cggctcggca attgacctgc cggtttacag ctatgttgaa catacgcgta
    32281 tgaaagaaac ggtgacggtt gagccgaaga aggtcatcat tctcgaaggc attttgttgc
    32341 tgacggatgc acgtttgcgt gacgaactta acttctccat tttcgttgat accccgctgg
    32401 atatctgcct gatgcgccgc atcaagcgtg acgttaacga gcgtgggcgt tcaatggatt
    32461 ccgtgatggc gcaatatcaa aaaaccgtgc gcccgatgtt cctgcaattc attgagcctt
    32521 ctaaacaata tgcggacatt atcgtgccgc gcggcgggaa aaaccgcatc gcgatcgata
    32581 tattgaaagc gaaaataagt cagttctttg aataagcttg ataaattgtg taccgttcag
    32641 tgataacctg gtatgccctt gacgtaattg gcgttaaggg agtgatgcgc gaaaggagaa
    32701 aatgccatgc gtctgtgtga ccgagatatt gaagcctggc ttgatgaagg ccgtttgtcg
    32761 atcaacccac gtccgccagt ggagcgtatt aacggcgcga cggtggatgt acgcctgggc
    32821 aataaatttc gtaccttccg tggtcacacg gcagcgttta tcgatctgag cggtcccaaa
    32881 gatgaagtga gcgccgcgct tgaccgcgtg atgagcgatg agatcgttct cgatgaaagc
    32941 gaggcgttct atcttcaccc aggagagctg gcgctggcgg tgacgctgga gtcggtgacg
    33001 ctgccagccg atctggtggg ctggctggac gggcgttcct cactggcgcg tctggggctg
    33061 atggtgcacg tcaccgcgca ccgtatcgat ccgggctggt ctggttgcat tgtgctggag
    33121 ttctacaact ccggtaagct gccgctggcg ctgcgtccgg gcatgttaat tggtgcgctg
    33181 agctttgagc cgctttccgg cccggcggcg cgaccttaca accgccgtga agatgcgaag
    33241 tatcgcaacc agcagggcgc ggtagccagc cgaatcgata aagactaatt caggcccatt
    33301 gaggatacca tgagacgatt tctgacgacg ctgatgatac tcctggtcgt gctggtggcc
    33361 gggttatctg cgttagtgtt gctggtgaat ccgaatgatt tccgcgacta tatggtcaag
    33421 caagttgctg cacgtagcgg ttatcaattg cagctcgacg ggccactgcg ttggcacgtc
    33481 tggccgcagc ttagtatcct ctccgggcga atgtctctca ccgcccaggg cgcgagccag
    33541 ccactggttc gcgccgacaa catgcgcctg gacgtggcgc ttttaccact actgagtcat
    33601 caactgagcg ttaagcaggt gatgctaaaa ggggcagtga tccaactgac gccgcagacg
    33661 gaagcggtgc gcagtgaaga cgctccggtt gcaccgcgcg acaatacctt gccggatctg
    33721 tcagacgatc gcggatggtc gtttgatata tccagtctta aggtggcgga cagcgtgctg
    33781 gtgttccagc atgaagatga cgagcaggtg acaatccgca atatccgcct gcaaatggaa
    33841 caagatcccc aacatcgtgg ctcatttgag ttctccgggc gggttaatcg cgatcagcgc
    33901 gatctcacga tatcccttaa cggtacggta gatgcttctg attatccgca tgatttaacg
    33961 gcggctattg aacaaattaa ctggcagttg cagggtgccg atttaccaaa acaaggtatt
    34021 caggggcagg ggagtttcca ggcccagtgg caggagtcac gtaaacgcct ttcatttaac
    34081 caaattagtt tgaccgccaa tgatagtacg ctgagcgggc aagcacaggt cacgctgaca
    34141 gagaaaccgg aatggcagct gaggctgcaa ttcccgcaac tgaatcttga caacctcatc
    34201 ccgcttaatg aaaccgcgaa tggtgaaaac ggtgccgcgc agcaggggca gagccaatca
    34261 acgttgccgc gcccggttat ttcttcgcgt attgatgaac cggcctatca gggactgcaa
    34321 ggctttacgg ctgatatttt gttgcaggcc agtaacgtgc gctggcgcgg aatgaatttt
    34381 acagatgttg ccacgcaaat gaccaacaag tcgggtttgc tggaaattac tcaactacag
    34441 ggcaagctta acggtggaca ggtttcactg ccgggcacgc tggacgcgac atcaataaat
    34501 ccgcggataa acttccagcc acggctggaa aacgttgaga ttggcaccat tctgaaggcg
    34561 tttaactatc cgatttcgtt gaccggaaaa atgtcattgg ctggtgattt ctccggtgct
    34621 gacatagatg ccgacgcatt ccgccacaac tggcaaggac aggcacatgt cgaaatggcc
    34681 gatacgcgca tggaagggat gaacttccag cagatgattc agcaagcggt agaacgtaat
    34741 ggtggtgatg tgaaggccgc tgaaaacttc gataacgtaa cgcgtcttga ccgctttacc
    34801 accgatttga cgttgaagga tggcgtcgtg acgttaaacg acatgcaagg tcaatcgcct
    34861 gtgctggcgc ttacagggga aggcatgttg aatctggcag atcaaacctg cgacacccag
    34921 tttgatattc gggtcgtggg tggctggaac ggggaaagca aactgattga tttcctcaaa
    34981 gaaacgccag taccgctgcg ggtttatggc aactggcagc aactcaatta cagtctgcaa
    35041 gtggatcagt tactgcgcaa acatctacag gacgaagcga aacgtcgcct gaatgactgg
    35101 gccgagcgga ataaagattc ccgcaatggc aaagatgtga agaagttgct ggagaagatg
    35161 taagacaagt tatatagatg gcggcatcaa tttgtcgcca tctgtattta tagcactaag
    35221 cagcatgtgg atattgaact tcaatacgtt gaatatgtag tcctgcatga gccatcatgg
    35281 tgatcaattt atcgatacta aagatctgga ttttacctct ggcaagttca gagatgcgag
    35341 gttgagtgac ccctaaagca gaagctgcct gagcctggct aaacccttgt tgttcaatcc
    35401 agttattaat gatcgttaca agctcggcac ggatccgcat attttccgct tgttcagggg
    35461 tgtcactcac cgcatcccac acactatcaa aggtttcaat tttcattttt ccgctcctga
    35521 acaagttctt tatagcgtcg tttggctaaa tcaatatccg attgactggt agtctgtgtt
    35581 ttcttttgaa aacaatgcaa tacgtaaacc gcttcttcaa attttgcgac atacatgacg
    35641 cgatagatcc catcagcatc gcgagtacga atctctcgca caccagggcc gatagttgaa
    35701 aaaggtttcc agtcgtatgg gtttaaccct tgttggatcc tgtcgagttg atagccaatt
    35761 tcctgtcgag acgattctgg aaatgcgcgt aaatcgtcaa gactggtatc aagaaaagcc
    35821 agcttttttc tcattactcc atccttagat tatactaaat tttgtataaa tcaaatgcgc
    35881 agtttgaacc tgcgcatttg atgttttatc acacctcgta ctccatctcg ccatccttac
    35941 gcagcggtat aatctgcacc ttctgcacgc gatggctttc cacctgcaac gttttaagca
    36001 aataatcccc cacctgaact tcttcgccag gttttggaat acgctgcaaa tattccatca
    36061 atagcccggc aatggtgtga tattcacgtt tttcatccag cggcagcggc acatattgca
    36121 ccagatcttc cagcggcata tgaccattcg ccgtccagga accgtccgca ttcttctgaa
    36181 tatcatggcg ggcgtcgatc tcttccactt cgttcggtaa gttaccggca atggtttcag
    36241 tgacgtcact taatgtcaca atcccttcca ccgagccaaa ctcatccacc acaaaagcaa
    36301 agtgtgtgcg ggcattacgg aactgctcca gggcaggtaa caacggcaag gtttccggga
    36361 acaccagcgg ctggcgaatt aacacccgca ggttgagcgg ttcgccgcgc agtgattgtt
    36421 gtaacaggtc gataacgtga acaacaccga gcaaatcttc cgcgtcatcg ccatcagtaa
    36481 ccaccagccg cgtatgctga tttcgctcca gcaactggcg gatctcgtct tccggcgcgt
    36541 tgagatcgat atgctcaata tcgtggcgcg acgtcataat gctgctgacg gtacgctggt
    36601 taagattaag tacccgctca atcatccgcc gttcctgcgg attaaagatc tgctggttac
    36661 catgatccac cagcatggac gcggtttcgg cgtccagttc cgcatcctct ttttgcccgc
    36721 taagcaggcg cattaccgct tcggtagtcc gctggcgcag tgtctggtta gcggaaagaa
    36781 aacgtcgccg gttaaagata gccaactgat tgagcgcctc gatcatcacc gagaaaccaa
    36841 tggcagcgta caggtagcct ttcggaatga cgaagccgaa accttctgcc accaggctaa
    36901 agccaatcat caacaggaag cttaagcaga gaataacgat ggtcgggtga ctgttaacaa
    36961 attgcgttaa cggcttgctg gccatcaaca tcaggctgat agcgataacc acggcggcca
    37021 tcatgaccag taaatggtcg accatcccga cggcggtaat cactgagtcg agtgagaaga
    37081 tggcgtccag taccacaatt tgtgtcacca ccccccagaa cttcgcacct ttgcgttgtg
    37141 tgggattatt gctgtctttc ccttccagcc gttcgttcag ctccatcgtg gctttgaaca
    37201 acaggaagaa accaccaaac agcatgatta agtcgcgggc gctaaaggta aacgagcgga
    37261 agctgaacag cggttgagtc agggtgacca gccaggagat tgacgccagc agtaacaggc
    37321 gcattaacat cgccagcaac agcccggtaa cccgtgcgcg gtcgcgctgc ttcggcggta
    37381 gtttttcggc gaggatggca ataaagacca ggttatcaat gccgaggacc agttcgatca
    37441 caatcagcgt gattaacccg gcccagatag acggatcggc aatccattcc atagtaagta
    37501 caaaacctat tcgttgtatg acaaatgtca caattcgatc atggataaat cggggcaaaa
    37561 atgcaatgtt gcagcactat ttatctcata ttatttgagg gtaataacct cacattatcc
    37621 ctgaattaaa agtggtaata ataaaacatt atttaagaaa tatcgcatgt ggagacgcaa
    37681 tattaaatat tgtcaaccta aagaaactcc taaaaaccat attgaatgac acttaatata
    37741 attcttaaaa atagccaatt gccgaattgt taccttgcct gctattccgt tagctgtaac
    37801 acttcctcct gcattattgg agagccaata ttcaattacc ataatgtcac cttatattcg
    37861 gaagttaatt tccgcaataa ccctttctca tcaacgattg tatgcagaga gggtttgaaa
    37921 aattaagcta aatcagtgta ttggtagcta aaaagccagg ggcggtagcg tgtctggatg
    37981 cctgaaagac cagtctgaat tattctgtca atagcctgcg gatgaaatca atttttttag
    38041 gactgatgcc agttaaattt ttattcaatt aactgcaagg taaatgtgca tggcgacatt
    38101 gctattaata gtgcacagga taattactct gccaaagtga taaataaaca atgatgaaat
    38161 ccaaaatgaa attgatgcca ttattggtgt cagtaacctt gataagcggt tgcacagtac
    38221 ttccgggcag caatatgtcg acgatgggca aagacgtcat caaacagcag gacgctgatt
    38281 tcgatctcga caaaatggtg aatgtttatc cgctgacccc gcgcctgatt gaccaattac
    38341 gcccacgccc gaatgtagcg cgccccaata tgacgctgga aagtgagatc gcgaattacc
    38401 agtatcgcgt cgggccgggg gacgttctta atgtcaccgt ctgggatcac ccggaactca
    38461 ccacgccagc cggtcagtac cgcagctcca gcgacaccgg caactgggta cagcctgacg
    38521 gcactatgtt ttacccgtat atcggcaagg ttcacgtagt cgggaaaacg ctcgctgaaa
    38581 tccgcagtga tattaccggg cgcttagcga cgtacatcgc tgacccgcag gtggacgtta
    38641 atatcgccgc cttccgctca caaaaggcct atatctcagg tcaggtgaat aaatctggtc
    38701 aacaggcgat caccaacgta ccactgacca ttctcgacgc catcaacgcc gcaggtggcc
    38761 tgaccgacac cgctgactgg cgcaacgtgg tgctaacaca caatggtcgt gaagagcgaa
    38821 tttctttgca ggcgctgatg caaaacggcg acctcaatca gaaccgactg ctttaccccg
    38881 gcgatattct ctacgtgccg cgtaatgatg atctgaaagt gtttgtgatg ggtgaagtga
    38941 agaaacagag caccctgaaa atggacttta gcggcatgac cctgaccgaa gccctgggca
    39001 atgctgaagg catcgacatg accacctcca acgccagcgg catctttgtc attcgtccgc
    39061 tgaaaggcga gggcgggcgt aacggcaaaa ttgccaatat ctaccagctg gatatgtccg
    39121 atgccacgtc gctggtgatg gcgacagaat tccgcctgca accttatgac gtggtgtatg
    39181 tcaccaccgc cccggtttcc cgctggaacc gtctgatcaa tcagttgctg ccaactatta
    39241 gcggtgtccg ttacatgacg gatacagcca gcgacattca taactggtaa tcgtcatgtt
    39301 taacaacatc ttagttgtct gtgtcggcaa tatttgccgt tccccgacgg cggaacgctt
    39361 actgcaacgt tatcacccgg agctgaaagt ggagtccgct ggactcggcg cgctggtcgg
    39421 taagggcgct gatcctaccg ctatcagcgt cgccgcagaa catcaactgt ctctggaagg
    39481 ccactgtgca cgacaaatca gccgccgcct gtgtcgcaac tatgacctga ttttgaccat
    39541 ggaaaagcgc catatcgaac gcttatgcga gatggcaccg gagatgcgcg gcaaagtgat
    39601 gctgtttggt cactgggata acgaatgtga aatccccgat ccgtatcgca aaagccggga
    39661 aacgtttgca gcggtgtaca cattacttga acggtctgcc cgccagtggg cgcaggcatt
    39721 gaacgcagag caggtataag aatgacagaa aaagtaaaac aacatgccgc tccggtaacg
    39781 ggcagtgatg aaatcgatat tggtcgcctg gtcggcaccg tcattgaagc gcgctggtgg
    39841 gtgattggca tcaccgctgt attcgccctt tgtgccgtgg tttacacctt cttcgccacg
    39901 ccgatttata gtgccgacgc actggtacaa atcgagcaaa gcagcggcaa ttcgttagtg
    39961 caggatatcg gatcggcgtt agccaacaaa ccgcctgcat cggacgccga gatccagttg
    40021 attcgttcgc gcctggtgct tggtaaaacg gtggatgatc tcgacctcga tattgcggtg
    40081 agcaaaaaca cgttcccgat tttcggtgcg ggctgggatc gcctgatggg acgccagaac
    40141 gagacggtga aagtgactac ctttaaccgt ccgaaagaga tggaggatca ggtgtttacg
    40201 cttaatgtgc tggacaacaa aaactacacc ctgagcagcg atggcggctt tagcgcccgt
    40261 gggcaagcgg gccagatact gaaaaaagaa ggcgtcacgc tgatggttga agccattcac
    40321 gcccgcccgg gcagtgagtt taccgtcacc aaatactcca cgctggggat gatcaatcaa
    40381 ctgcaaaaca gcctgacggt aacggagaac ggcaaagacg caggcgtact gagcctgact
    40441 tataccggtg aagatcgcga acagatccgc gacattctta acagcatcgc ccgtaactat
    40501 caggaacaaa atattgagcg caaatcggcg gaagcgtcga aaagcctcgc tttcctcgcg
    40561 caacagttac cggaagtacg tagccgcctt gatgttgccg aaaacaaact gaatgccttc
    40621 cgtcaggata aagattctgt tgatctgccg ctggaagcga aagcggtgct cgattcgatg
    40681 gtgaacatcg acgcccagtt gaacgaactg acctttaaag aggcggaaat ctccaagctg
    40741 tacaccaaag ttcaccccgc gtaccgcacg ctgctggaga aacgtcaggc gctggaagac
    40801 gaaaaagcca aacttaatgg tcgcgtaacg gcgatgccga aaacccagca ggaaattgtc
    40861 cgtctgaccc gcgatgtcga gtctggtcag caggtctata tgcaactgct gaataaagag
    40921 caggagctga aaatcaccga ggccagcacc gtcggcgatg tgcgcattgt tgacccggca
    40981 atcactcagc ctggtgtgct aaaaccgaag aaagggctga ttatccttgg ggcgattatc
    41041 cttggcctga tgctctctat cgtgggggtg ctgctgcgct cgttgtttaa tcgcggcatc
    41101 gaaagcccgc aggtgctgga agaacacggt atcagcgtct atgccagcat cccgctgtcg
    41161 gagtggcaga aagcgcgcga tagcgtcaaa accatcaaag ggattaaacg ctataaacag
    41221 agccagctac tggcggtggg gaatccaacc gatctggcga ttgaagccat ccgcagcctt
    41281 cgtaccagtt tgcacttcgc gatgatgcag gcgcagaaca atgtgttgat gatgaccggg
    41341 gttagcccgt caatcggtaa aacctttgtc tgcgccaacc tggcggcggt gatcagccag
    41401 accaataaac gcgtgttgtt gatcgactgc gatatgcgca aaggctacac ccatgagctg
    41461 ttgggcacta ataacgttaa tggcctgtcg gaaattctga ttggtcaggg cgatattact
    41521 acagctgcta aaccgacctc tattgccaaa tttgacctga tcccgcgcgg tcaggtaccg
    41581 ccaaatcctt ctgaactgtt gatgagcgaa cgctttgccg aactggtgaa ctgggcgagt
    41641 aaaaactacg acctggtgtt gattgatacg ccgccgattc tggcagtgac cgatgcggca
    41701 attgttggtc gtcatgtcgg aaccacgtta atggtggcgc gttatgcggt caacacattg
    41761 aaagaagtgg aaaccagtct gagccgcttt gagcaaaacg gtattccggt gaaaggggtg
    41821 attctgaact ccatcttccg ccgcgccagc gcgtatcagg attatggcta ttacgaatac
    41881 gaatataagt cggatgcgaa ataacgaggc ctgcattcgc accgccccgt aggccggata
    41941 aggcgctcac gccgcatccg gcaagcaaac cagctcataa gccgggagta caacctatga
    42001 aagacaatcc gctgatctca atctatatgc cgacctggaa ccgccaacaa ctggcgattc
    42061 gggcgataaa atcggtcttg cgccaggact acagcaactg ggagatgatc atcgtggatg
    42121 attgctccac ctcctgggaa caactgcaac agtacgtcac cgccctcaac gatccgcgta
    42181 ttacctacat tcacaacgac ataaacagcg gggcgtgcgc ggtacgtaac caggcgatta
    42241 tgctggcgca aggggaatac attaccggga tcgatgacga tgatgaatgg acgcccaacc
    42301 gcctgagcgt cttcctcgcc cataaacagc aactggtcac tcacgccttt ttgtacgcta
    42361 acgattacgt ttgccagggc gaagtttatt cccaaccggc aagcttacca ctgtatccca
    42421 aatcgccgta ctcacgccgc ctgttctata aacgcaatat cattggcaat caggtcttta
    42481 cctgggcatg gcgtttcaaa gagtgtttgt tcgataccga actgaaagcg gcacaggatt
    42541 acgacatctt cctgcggatg gtggtggagt atggcgaacc gtggaaagtg gaagaggcga
    42601 cgcagatcct gcatatcaat cacggtgaaa tgcagatcac ctcgtcaccg aaaaaattct
    42661 ccggatactt ccacttttac cgcaagcaca aagacaaatt cgaccgtgcc agtaaaaaat
    42721 atcagctgtt taccctctac cagatccgca ataaacgtat gacctggcgc actttgctga
    42781 cgctgctgtc ggtgcgtaac ggcaaacgtc tggctgatgg tattcggggg cgctaaatat
    42841 gctggaagat ctacgcgcca acagctggag tttacgcccg tgctgcatgg ttcttgccta
    42901 tcgcgtcgct catttttgtt cggtgtggcg caaaaagaac gttctcaaca atctttgggc
    42961 ggccccgctg ctggtgctgt atcgcattat caccgaatgc tttttcggtt atgaaatcca
    43021 ggctgccgcg accattggcc gccgctttac tatccatcac ggttacgccg tagtgatcaa
    43081 taaaaacgtg gtagcggggg atgatttcac cattcgccac ggcgtcacta tcggcaatcg
    43141 tggtgccgat aacatggcat gtccacacat tggcaacggc gtcgaactcg gtgccaacgt
    43201 cattattctt ggtgatatca cgcttggtaa caacgtcacc gtgggcgcgg gcagcgtggt
    43261 gctcgactct gtcccggaca acgcgctggt ggtgggagaa aaagcgcgag tgaaggtaat
    43321 taaatgaata ttctgcaatt taatgtgcga ctggcggaag gcggggcggc aggtgtggcg
    43381 ttagatctcc accagcgtgc gctgcaacag gggctggcgt cacattttgt ctacggctac
    43441 ggcaaaggcg gcaaagagag cgtcagccat cagaactatc cgcaggtcat caaacatacg
    43501 ccgcggatga ccgcaatggc gaatattgcc ctgtttcgtc tgtttaatcg cgatctgttt
    43561 ggcaatttca atgagttata tcgcaccatt actcgtacac cgggtccggt ggtcctgcat
    43621 tttcatgtgc tgcacagcta ctggctaaat cttaagagcg tggtgcgctt ttgcgaaaag
    43681 gtgaaaaacc acaaaccgga tgtcactctg gtctggacgc tgcacgacca ctggagcgtt
    43741 accgggcgct gcgcctttac cgacggttgt gaaggctgga aaacgggctg ccagaaatgc
    43801 ccgaccttaa ataactatcc gccggtgaag attgatcggg cgcaccagct ggtggcgggc
    43861 aaacgccagt tattccgtga gatgctggcg ctgggctgtc agtttatttc ccccagccag
    43921 catgtggctg acgctttcaa tagcctgtac ggtccagggc gttgccggat tatcaataac
    43981 ggtattgata tggcaaccga ggcgattctg gcggatttgc ctccggtgcg cgaaactcag
    44041 ggcaagccga aaatcgcggt ggtagcgcat gacctgcgtt acgacggcaa aactaaccag
    44101 caactggtac gcgagatgat ggcgctgggc gacaaaatcg aactgcatac ctttggtaag
    44161 ttctcgccgt tcaccgctgg caacgtggtt aatcacggct ttgaaactga caagcgcaag
    44221 ttgatgagcg cgctcaatca gatggatgcg ctggtgttca gttctcgcgt cgataactac
    44281 ccgctgattt tgtgtgaggc gttatcaatt ggcgtgccgg tgattgccac ccatagcgac
    44341 gcggcgcgtg aagtgttgca aaaatccggc ggtaaaaccg tcagcgaaga agaggtgctg
    44401 caactggtcc agttaagcaa accggaaatc gcgcaggcgt tatttggtac cacgctggct
    44461 gagttcagcc aacgcagccg cgccgcctac agtggacaac agatgctgga ggagtatgtc
    44521 aacttctatc agaatctgta gctacctgct gctgccgctg atctacctgc tggttaacgt
    44581 caaaatcacc cagcttggcg aaagtttccc catcaccatc gtcacttttt tacctgtctt
    44641 gttactgctg tttttagaac gcatcagcgt taaaaaattg atgattgcct tagggatttg
    44701 cgcgggactc acggcgttta actacctgtt tggtcagtcg ctggatgcca gtaaatacgt
    44761 cacttcaact atgctgtttg tctatatcgt gatcattatt ggtatggtgt ggagtattcg
    44821 ctttaaaacg atttcgccac acaaccatcg caagatatta cgtttctttt atctggtggt
    44881 cgggctggtg gtggcgctgg cggcggtgga gatggcgcaa attatcctca ccggcggcag
    44941 cagtattatg gagtcgattt cgaaatatct gatttacagc aacagctatg tgctgaattt
    45001 catcaaattc ggcggcaagc gcacgacagc actttatttc gaaccggcat ttttcgctct
    45061 ggcattaatc tcaatttggc tcagcatcaa acagtttggt atcaaaacgc ctaaaacaga
    45121 tgctatgatt ctcgcaggga taatattatc cggatcgttt tcaggggtta tgacctttat
    45181 cctgttttat ttgctggagt gggcatttca atatctgaat aaagaggcga ttaagaaaaa
    45241 gttaccgtta gcattgattt ctctggctgt attcctggtt ggtgtggtaa ttgcatttcc
    45301 ttatatttcc acccgtctgg gcgatttagg tacggaagga tcgtcatcgt attatcgtat
    45361 tgtcggtccg ctggtgatgg tcggttattc tttgacccat attgacggtg tagtcagatt
    45421 tggctcactt tatgaatatg tcgcatcatt cggaatattt aacggtgcgg atgtcggaaa
    45481 aaccatagac aatggtttgt atctgctgat tatttatttt tcttggttcg cggtgttttt
    45541 atcgctgtgg tacatgggga aagtgataaa aatgatgatc aacgcttttg gtgataaccg
    45601 caattttcgc gtgcaattat atctttttac tccggtatcg ctgtttttta ccggttcgat
    45661 atttagcccg gaatatgcat ttttaatcgt ctgtccgttt attttgcgaa aagcgttaaa
    45721 tattacgagg taagaataag aacatgttgc ttagcataat cactgtcgcg tttcgtaacc
    45781 tcgaagggat agtcaaaaca catgcctcgc tggcgcatct ggcgcaggcg gaagatatca
    45841 gcttcgaatg gattgttgtc gatggcggtt ccaatgacgg cactcgtgag tacctggaaa
    45901 atctcaatgg tatctataac ctacgctttg tcagcgagcc agataacggt atctacgacg
    45961 ccatgaacaa gggcattgcg atggcacaag gcaagttcgc gttgtttctc aactcgggcg
    46021 atatttttca tcaggatgcc gcatattttg tccgtaagtt aaaaatgcaa aaagataacg
    46081 tgatgatcac cggcgatgcg ctgctggatt ttggcgacgg gcataaaatt aaacgtagcg
    46141 ccaaaccggg ctggtatatt tatcacagcc tgcccgccag tcatcaggcg atatttttcc
    46201 cggtatccgg tttgaaaaaa tggcgttatg acctggaata taaagtttct tctgattacg
    46261 cgctggcagc caaaatgtat aaagcaggtt atgcatttaa aaaactcaat ggcctggtgt
    46321 ctgaattttc tatgggtggg gtatctacca ccaataatat ggaattgtgt gctgacgcga
    46381 aaaaagtcca acggcaaata ttacatgtgc ctggcttttg ggctgaatta tcctggcatt
    46441 tacgccagcg tactacctca aagacgaaag ccttatataa caaaagctga atataaggga
    46501 aaacgatgca ggatttaagc ggtttctcgg tgccgaaagg gttccggggc ggcaacgcta
    46561 ttaaagtgca attgtggtgg gcagtacagg caacaatatt tgcctggtcg ccacaagtat
    46621 tgtatcgctg gcgggcattt ttattacgtt tattcggcgc aaaaatagga aaaaacgtag
    46681 ttattcgtcc gtcagtaaaa attacctatc cgtggaaatt aaccttaggt gattacgtgt
    46741 gggtcggcga tgacgtcaat ttatataccc tcggtgaaat aaccattggc gcacattcgg
    46801 tgatatcgca aaaaagttat ttatgcaccg ggagccacga ccatgcaagt caacatttca
    46861 ccattaacgc cacgcctatt gtgattggcg agaaatgctg gctggcaacc gatgtttttg
    46921 ttgccccagg cgtcacgatt ggcgacggca ccgtcgtggg tgcgcgaagc agtgttttta
    46981 aatcgcttcc ggcaaatgtg gtttgccggg ggaatcccgc agtggtgata cgcaaacgcg
    47041 ttgaaactga ataaattcaa aaaaaataca gaggaataag acatgtcaaa agtcgctctc
    47101 atcaccggtg taaccggaca agacggttct tacctggcag agtttctgct ggaaaaaggt
    47161 tacgaggtgc atggtattaa gcgtcgcgca tcgtcattca acaccgagcg cgtggatcac
    47221 atttatcagg atccgcacac ctgcaacccg aaattccatc tgcattatgg cgacctgagt
    47281 gatacctcca acctgacgcg tattttgcgt gaagtgcagc cggatgaagt gtacaacctg
    47341 ggcgcaatga gccacgttgc ggtttctttt gagtcaccgg aatataccgc tgacgtcgac
    47401 gcgatgggta cgctgcgcct gctggaggcg atccgcttcc tcggtctgga aaagaaaacc
    47461 cgtttctatc aggcttccac ctctgaactg tatggtctgg tgcaggaaat tccgcagaaa
    47521 gagaccacgc cgttctatcc gcgctctccg tatgcggtcg ccaaactgta tgcctactgg
    47581 atcaccgtca actaccgtga atcctacggc atgtatgcct gtaacggtat tctcttcaac
    47641 catgaatccc cgcgccgcgg cgaaaccttc gttacccgca aaatcacccg cgcaatcgcc
    47701 aacatcgccc aggggctgga gtcgtgcctg tacctcggca atatggattc cctgcgtgac
    47761 tggggccatg ccaaagacta cgtaaaaatg cagtggatga tgctgcaaca ggaacagcca
    47821 gaagatttcg ttatcgcgac cggcgttcag tactccgtac gtcagttcgt agaaatggcg
    47881 gcagcacagc tgggcatcaa actgcgcttt gaaggcacgg gcgttgaaga gaagggcatt
    47941 gtggtttccg tcaccgggca tgacgcgccg ggtgttaaac cgggtgatgt gattatcgcc
    48001 gttgatccgc gttacttccg tccggctgaa gttgaaacgc tgctcggcga cccgaccaaa
    48061 gcgcacgaaa aactgggctg gaaaccggaa atcaccctca gagagatggt gtctgaaatg
    48121 gtggctaatg acctcgaagc ggcgaaaaaa cactctctgc tgaaatctca cggctacgac
    48181 gtggcgatcg cgctggagtc ataagtatga gtagacaacg catttttatt gctggccatc
    48241 gcgggatggt cggttccgcc atcaggcggc agctcgaaca gcgcggtgat gtggaacagg
    48301 tattacgcac ccgcgacgag ctgaacctgc tggacagccg cgccgtgcat gatttctttg
    48361 ccagcgaacg tattgaccag gtctatctgg cggccgcgaa agtgggcggc attgttgcca
    48421 acaacacgta tccggcggat ttcatctatc agaacatgat gattgagagc aacatcattc
    48481 acgccgcgca tcagaacgac gtgaacaaac tgctgtttct cggatcgtcc tgcatctacc
    48541 cgaaactggc aaaacagccg atggcagaaa gcgagttatt gcagggcacg ctggagccga
    48601 ccaacgagcc ttacgccatt gccaaaatcg ccgggatcaa actgtgcgaa tcatacaacc
    48661 gccagtacgg acgcgattac cgctcagtca tgccgaccaa cctgtatggc ccgcatgaca
    48721 acttccaccc gagtaattcg catgtgatcc ctgcattgct gcgtcgcttc cacgaggcga
    48781 cggcacagaa tgcaccggac gtggtggtgt ggggcagcgg tacgccgatg cgagaatttc
    48841 tgcacgtcga tgatatggcg gcggcgagca ttcatgtcat ggagctggcg catgaagtct
    48901 ggctggagaa cacccagccg atgctgtcgc acattaacgt cggcacgggc gttgactgca
    48961 ctatccgcga gctggcgcaa accatcgcca aagtggtggg ttacaaaggt cgggtggttt
    49021 ttgatgccag caaaccggat ggtacgccgc gcaaactgct ggatgtgacg cgcctgcatc
    49081 agcttggctg gtatcacgaa atctcactgg aagcggggct tgccagcact taccagtggt
    49141 tccttgagaa tcaagaccgc tttcgggggt aatgatgttt ttacgtcagg aagactttgc
    49201 cacggtagtg cgctccacac cgcttgtctc tctcgacttt attgtcgaga acagtcgcgg
    49261 cgagtttctg cttggcaaaa gaaccaaccg cccggcgcag ggttactggt ttgtgccggg
    49321 agggcgcgtg cagaaagacg aaacgctgga agccgcattt gagcggctga cgatggcgga
    49381 actggggctg cgtctgccga taacagcagg ccagttttac ggtgtctggc agcactttta
    49441 tgacgataac ttctccggca cggatttcac cactcactat gtggtgctcg gttttcgctt
    49501 cagagtagcg gaagaagaac tgttactgcc ggatgagcag catgacgatt accgctggct
    49561 gacgccggac gcgctgctcg ccagtaacga tgttcacgct aacagccgcg cctattttct
    49621 cgctgagaag cgtgccggag tacccggatt atgaaaatac tggtctacgg cattaactac
    49681 tcgccggagt taaccggcat cggcaaatac accggcgaga tggtggaatg gctggcggcg
    49741 caaggtcatg aggtgcgggt tattaccgca ccgccttact acccgcagtg gcaagtgggc
    49801 gagaactatt ccgcctggcg gtacaaacga gaagaggggg ccgctacggt gtggcgctgc
    49861 ccgctgtacg tgccaaaaca gccgagcacc ctgaaacgcc tgttgcatct cggcagtttt
    49921 gccgtcagca gttttttccc gttgatggcg caacgtcgct ggaagccgga tcgcattatc
    49981 ggcgtagtgc caacgctgtt ttgcacgccg ggaatgcgcc tgctggcgaa actctctggc
    50041 gcgcgtaccg tgctgcatat tcaggattac gaagtagatg ccatgctggg gctgggcctt
    50101 gccggaaaag gcaaaggcgg caaagtggca cagctggcaa cggcgttcga acgtagcgga
    50161 ctgcataacg tcgataacgt ttccacgatt tcgcgttcga tgatgaataa agccatcgaa
    50221 aaaggcgtgg cggcggaaaa cgtcatcttc ttccccaact ggtcggaaat cgcccgtttt
    50281 cagcatgttg cagacgccga tgttgatgcc cttcgtaacc agcttggcct gccggataac
    50341 aaaaaaatca ttctttactc cggcaatatt ggtgaaaagc aggggctgga aaacgttatt
    50401 gaagcagccg atcgcctgcg cgatgaaccg ctgatttttg ccattgtcgg gcagggcggc
    50461 ggcaaagcgc ggctggaaaa aatggcgcag cagcgtggac tgcgcaacat gcaatttttc
    50521 ccgctgcaat cgtatgacgc tttacccgca ctgctgaaga tgggcgattg ccatctggtg
    50581 gtgcaaaaac gcggcgcggc agatgccgta ttgccgtcga aactgaccaa tattctggca
    50641 gtaggcggta acgcggtgat tactgctgaa gcccacacag aactgggaca gctttgcgaa
    50701 acctttccgg gcattgcggt ttgcgtagaa ccggaatcgg ttgaggcgct ggtggcgggg
    50761 attcgtcagg cgctcctgct gcccaaacac aacacggtgg cacgtgaata tgccgaacgc
    50821 acgctcgata aagagaacgt gttacgtcaa tttataaatg atattcgggg ataattatgg
    50881 cgcagtcgaa actctatcca gttgtgatgg caggtggctc cggtagccgc ttatggccgc
    50941 tttcccgcgt actttatccc aagcagtttt tatgcctgaa aggcgatctc accatgctgc
    51001 aaaccaccat ctgccgcctg aacggcgtgg agtgcgaaag ccctgtggtg atttgcaatg
    51061 agcagcaccg ctttattgtt gcggaacaac tgcgtcaact gaacaaactc actgagaaca
    51121 ttattctcga accggcaggg cgtaacacag caccggccat tgcgctggcg gcgctggcgg
    51181 caaaacgtca tagcccggag aacgacccat taatgctggt gctggcggcg gatcatgtga
    51241 ttgccgatga agacgcattc cgcgccgccg tgcgtaacgc tatgccgtat gccgaagcgg
    51301 gcaagctggt gaccttcggc attgtgccgg atctacctga aaccggatat ggctatattc
    51361 gtcgcggtga agtgtcggcg ggtgagcagg atacggtggc ctttgaagtg gcgcagtttg
    51421 tcgaaaaacc aaatctggaa accgcgcagg cctatgtggc aagcggcgaa tattactgga
    51481 acagcggtat gttcctgttc cgtgccggac gctatctcga agaactgaaa aaatatcgcc
    51541 cggatattct cgacgcctgt gaaaaagcga tgagcgccgt cgatccggat ctcgatttta
    51601 ttcgcgtgga tgaagacgcg tttctggcct gcccggaaga gtcggtggat tacgaggtca
    51661 tggaacgtac ggcagatgcc gttgtggtgc cgatggatgc gggctggagc gatgtcggct
    51721 cctggtcctc attatgggag atcagcgccc acaccgccga gggcaacgtt tgccacggcg
    51781 atgtgattaa tcacaaaact gaaaacagct atgtgtatgc cgaatctggc ctggtcacca
    51841 ccgtcggggt gaaagatttg gtggtagtgc agaccaaaga tgcggtgctg attgccgacc
    51901 gtaacgcggt gcaggatgtg aaaaaagtgg tcgagcagat caaagccgat ggtcgccatg
    51961 agcatcgggt gcatcgcgaa gtctatcgcc cgtggggcaa atatgactct atcgacgcgg
    52021 gcgaccgcta ccaggtgaaa cgcatcaccg tgaaaccggg cgaaggcttg tcggtacaga
    52081 tgcaccatca ccgcgcggaa cactgggtgg ttgtcgcggg aacggcaaaa gtcaccattg
    52141 acggtgatat caaactgctt ggtgaaaacg agtcgattta tattccgctg ggggcgacgc
    52201 actgcctgga aaacccgggg aaaattccgc tcgatttaat tgaagtgcgc tccggctctt
    52261 atctcgaaga ggatgatgtg gtgcgcttcg cggatcgcta cggacgggtg taaacgtcgc
    52321 atcaggcgct gattgtcgga tgcggcgtga acgccttatc cgacctacgg ttcggttttg
    52381 taggcctgat aagacgcggc agcgtcgcat caggcaatga atgcgaaacc gcggtgtaaa
    52441 taacgacaaa aacaaaattg gccgtttcgg tcagggccaa ctattgcctg aaaaagggta
    52501 acgatatgaa aaaattaacc tgctttaaag cctatgatat tcgcgggaaa ttaggcgaag
    52561 aactgaatga agatattgcc tggcgcattg gtcgtgccta tggcgaattt ctcaaaccga
    52621 aaaccattgt gttaggcggt gatgcccgtc tcaccagcga aaccttaaaa ctggcgctgg
    52681 caaaaggttt acaggatgcg ggcgttgacg tgctggatat tggtatgtcc ggcaccgaag
    52741 agatctattt cgccacgttc catctcggcg tggatggcgg cattgaagtt actgccagcc
    52801 ataacccgat ggattacaac ggcatgaaac tggtgcgcga aggggctcgc ccgatcagcg
    52861 gcgataccgg actgcgcgat gtccagcgtc tggcagaagc caacgacttt cctcccgttg
    52921 atgaaaccaa acgcggtcgc tatcagcaaa tcaatctgcg tgacgcttac gttgatcacc
    52981 tgttcggtta tatcaacgtc aaaaacctta cgccgctcaa gctggtgatc aactccggga
    53041 acggcgcggc gggtccggtg gtggacgcca tcgaagcccg ctttaaagcc ctcggcgcac
    53101 cggtggaatt gatcaaagtg cacaacacgc ccgacggcaa tttccccaac ggtattccta
    53161 acccgctgct gccggaatgt cgcgacgaca cccgcaatgc ggtcattaaa cacggcgcgg
    53221 atatgggcat tgcttttgat ggcgattttg accgctgttt cctgtttgac gaaaaagggc
    53281 agtttatcga gggctactac attgtcggtc tgctggcaga agcgttcctc gaaaaaaatc
    53341 ccggcgcgaa gatcatccac gatccacgtc tctcttggaa taccgttgat gtggtgactg
    53401 ccgcaggcgg caccccggta atgtcgaaaa ccggacacgc ctttattaaa gaacggatgc
    53461 gcaaggaaga cgccatctac ggtggcgaaa tgagcgccca ccattacttc cgtgattttg
    53521 cttactgcga cagcggcatg atcccgtggc tgctggtcgc cgaactggtg tgcctgaaag
    53581 gaaagacgct gggcgaactg gtgcgcgacc ggatggcagc gtttccgtca agcggtgaga
    53641 tcaacagcaa actggcgcaa cccgttgagg cgattaaccg cgtggaacag cattttagcc
    53701 gtgaggcgct ggcggtggat cgcaccgatg gcatcagcat gacctttgcc gactggcgct
    53761 ttaatctgcg ctcctccaac accgaaccgg tggtgcggtt gaatgtggaa tcacgcggtg
    53821 atgtgccgct gatggaagcg cgaacgcgaa ctctgctgac gttgctgaac gagtaatgtc
    53881 ggatcttccc ttaccccatt gcgggtaagg ggctaataac aggaacaacg atgacaaatc
    53941 taaaaaagcg cgagcgagcg aaaaccaatg catcgttaat ctctatggtg caacgctttt
    54001 cagatatcac catcatgttt gccggactat ggctggtttg cgaagtgagc ggactgtcat
    54061 tcctctacat gcacctgttg gtggcgctga ttacgctggt ggtgttccag atgctgggcg
    54121 gcatcaccga tttttatcgc tcatggcgcg gtgttcgggc agcgacagaa tttgccctgt
    54181 tgctacaaaa ctggacctta agcgtgattt tcagcgccgg actggtggcg ttcaacaatg
    54241 atttcgacac gcaactgaaa atctggctgg cgtggtatgg gctgaccagt atcggactgg
    54301 tggtttgccg ttcatgtatt cgcattgggg cgggctggct gcgtaatcat ggctataaca
    54361 agcgcatggt cgcggtggcg ggggatttag ccgccgggca aatgctgatg gagagcttcc
    54421 gtaaccagcc gtggttaggg tttgaagtgg tgggcgttta ccacgacccg aaactgggcg
    54481 gcgtttctaa cgactgggcg ggtaacctgc aacagctggt cgaggatgct aaagcaggca
    54541 agattcataa cgtctatatc gcgatgcaaa tgtgcgacgg cgcgcgagtg aaaaaactgg
    54601 tccatcaact ggcggacacc acctgttcgg tgctgctgat ccccgatgtc tttaccttca
    54661 acattctcca ttcacgtctt gaagagatga acggcgttcc ggtggtgccg ctttacgaca
    54721 cgccgctttc cggggttaac cgcctgctca aacgtgcgga agacattgtg ctggcgacgc
    54781 ttatcctgct gctgatctcc ccggtgctgt gctgcattgc gctggcggtg aaactcagtt
    54841 cacctgggcc ggttattttc cgccagactc gctacggcat ggatggcaag ccgatcaaag
    54901 tgtggaagtt ccgttccatg aaagtgatgg agaacgacaa agtggtgacc caggcgacgc
    54961 agaacgatcc gcgcgtcacc aaagtgggga actttctgcg ccgcacctcg ctggatgaat
    55021 tgccgcagtt tatcaatgtg ctgaccgggg ggatgtcgat tgtcggtcca cgtccgcacg
    55081 cggtggcgca taacgaacag tatcgacagc tcattgaagg ctacatgctg cgtcataagg
    55141 tgaaaccggg cattaccggc tgggcgcaga ttaacggctg gcgcggcgaa accgacacgc
    55201 tggagaaaat ggaaaaacgc gtcgagttcg accttgagta catccgcgaa tggagcgtct
    55261 ggttcgatat caaaatcgtt ttcctgacgg tattcaaagg attcgttaac aaagcggcat
    55321 attgatatga gcttacgtga aaaaaccatc agcggcgcga agtggtcggc gattgccacg
    55381 gtgatcatca tcggcctcgg gctggtgcag atgaccgtgc tggcgcggat tatcgacaac
    55441 caccagttcg gtttgcttac cgtgtcgctg gtgatcatcg cgctggcaga tacgctttct
    55501 gacttcggta ttgctaactc gattattcag cgaaaagaaa tcagccacct tgaactcacc
    55561 acgctgtact ggctgaacgt tgggctgggg cttgtggtgt gtgtggcggt gtttttgttg
    55621 agtgatctca ttggtgacgt gctgaacaac ccggacctgg caccgttgat taaaacattg
    55681 tcgctggcgt ttgtggttat cccccacggg caacagttcc gcgcgttgat gcaaaaggag
    55741 ctggagttca acaaaatcgg catgatcgaa accagcgcgg tactggcggg cttcactttt
    55801 acggtggtta gcgcccattt ctggccgctg gcgatgaccg cgatcctcgg ttatctggtc
    55861 aatagtgcgg tgagaacgct gctgtttggc tactttggcc gcaaaattta tcgccccggt
    55921 ctgcatttct cgctggcgtc tgttgcgccg aacttacgct ttggtgcctg gctgacggcg
    55981 gacagcatca tcaactatct caataccaac ctttcaacgc tggtgctggc gcgtattctc
    56041 ggcgcgggcg tggcaggggg atacaacctg gcgtacaacg tggccgttgt gccgccgatg
    56101 aagctgaacc cgatcatcac ccgtgtgttg tttccggcat tcgccaaaat tcaggacgat
    56161 accgaaaagc tgcgcgtcaa cttctacaag ctgctgtcgg tggtggggat tatcaacttt
    56221 ccggcgctgc tcgggctgat ggtggtgtca aataactttg taccgctggt atttggcgaa
    56281 aagtggaaca gcattattcc ggtgctgcaa ttgctgtgtg tggtgggtct gctgcgctcc
    56341 gtaggtaacc cgattggttc gctgctgatg gcgaaagcgc gggtcgatat cagctttaaa
    56401 ttcaacgtat tcaaaacatt tctgtttatt ccggcgattg ttattggtgg gcagatggcg
    56461 ggcgcaatcg gcgtcacgct cggtttcctg ctggtgcaga ttatcaacac cattctgagt
    56521 tacttcgtga tgattaaacc ggttcttggt tccagttatc gccagtacat cctgagtttg
    56581 tggctgccgt tttatctctc gctgccgacg ctggtggtca gttatgtgct gggcattgtg
    56641 ctgaaagggc aactggcgct ggggatgctg ctggcggtgc aaatagccgc gggcgtgctg
    56701 gcgtttgtgg tgatgattgt gctgtcgcgc catccgctgg tggtggaagt gaagcgccag
    56761 ttttgtcgca gcgaaaaaat gaaaatgctt ttacgggcgg ggtgagttgt tgttttcggt
    56821 tccgacaacg gttgccgggt gcagcgtaaa acaccttatc catcctacgg ttctgtgctc
    56881 gggtcttgta ggcctgataa gacgcgccag cgtcgcatca ggcaaaaaac ggcaacgaac
    56941 acaacaaaca cagcctcacc tggcatcaaa tttatgagga aaaaatgaaa ttacttattc
    57001 tgggcaacca cacttgcggc aatcgtggcg acagcgccat cttgcgcggc ttacttgatg
    57061 ccatcaacat cctcaatcca cataccgaag tggacgtgat gagccgctat ccggtcagtt
    57121 cttcctggct gctcaaccgc ccggtaatgg gtgatccgct gttccttcaa atgaaacaac
    57181 acaacagcgc ggcgggcgtt gtcgggcgcg ttaaaaaagt cctccgtcgc cgctatcagc
    57241 accaggtatt gctctcacgc gtcaccgaca ctggcaagct gcgcaatatc gccatcgccc
    57301 agggattcac cgacttcgtg cgcctactgt caggttacga cgccattatt caggtcggcg
    57361 gatcgttttt tgtcgatctc tacggcgtgc cgcagtttga acatgcactt tgcacattta
    57421 tggcgaaaaa gccgctgttt atgattggtc acagcgtcgg tcccttccag gatgagcaat
    57481 ttaaccaact ggcgaactac gtttttggtc actgcgacgc gctgatcttg cgcgaatcgg
    57541 tcagcctcga tctaatgaaa cgcagcaata tcaccactgc aaaagttgaa catggcgtcg
    57601 ataccgcgtg gctggtcgat caccacacag aagacttcac cgccagctat gccgtccaac
    57661 actggctgga cgttgccgca caacagaaaa cggtggcaat taccctgcgc gaactggcac
    57721 cgttcgacaa acgcctcggc accactcagc aagtgtatga aaaagccttt gccggggtgg
    57781 tcaatcgcat tctcgacgaa ggctatcagg tcattgcgct ctccacctgt acgggtatcg
    57841 acagctataa caaagacgac cgcatggtgg cgctcaacct gcgccagcac atcagcgatc
    57901 ctgcccgtta tcacgtagtg atggatgaac ttaacgatct ggaaatgggc aaaattctcg
    57961 gtgcctgtga actcaccgtc ggtacgcgcc tgcactccgc cattatctca atgaactttg
    58021 ctactccggc gattgccatc aactatgaac ataaatccgc cggggttatg cagcagctgg
    58081 gactaccgga gatggcaatt gatatccgtc atttattaga cggcagcctg caagcgatgg
    58141 ttgcggatac cttaggccag cttccggcgc tgaacgcacg acttagcgaa gccgtcagtc
    58201 gtgagcgtca gacgggaatg cagatggtgc agtccgtact ggaacgcatc ggggaggtga
    58261 aatgaaggtc ggcttcttct tattgaaatt tccgctgtcg tcagaaacct tcgttcttaa
    58321 ccaaatcact gcgtttattg atatgggctt tgaagtggag attgtcgcgc tgcaaaaagg
    58381 cgacacacaa aacacccacg tggcatggac taaatacaac cttgccgcca gaacccgctg
    58441 gttacaggac gaacctacgg gcaaagtggc gaaactgcgc caccgagcca gtcagacctt
    58501 gcgcggcatt catcgtaaaa atacctggca ggcgctcaac ctcaaacgct atggtgccga
    58561 gtcgtggaac ctgattttgt ctgccatttg cggccaggtc gcaacaccgt ttcgcgccga
    58621 tgtgttcatc gctcattttg gccctgcggg ggtaaccgca gcaaaactac gcgaactggg
    58681 tgtcattcac ggcaaaattg ccactatttt ccacggcatt gatatttcca gtcgggaagt
    58741 gctcaaccat tacactcccg aatatcaaca actgtttcga cgtggcgacc tgatgctacc
    58801 gataagcgat ctgtgggccg gaaggctgca aaaaatgggc tgcccgaggg aaaaaatcgc
    58861 cgtatcgcgc atgggcgtag atatgacgcg ctttagcccg cgtcccgtga aagcgcccgc
    58921 aacaccgctg gagattattt ccgtcgcacg cttaaccgag aaaaaaggtc tgcatgtggc
    58981 gattgaagcc tgccggcagt tgaaagagca gggcgtggca tttcgctatc gcatcctcgg
    59041 cattggcccg tgggaacgac gcctgcgcac gctcatcgaa caatatcaac tggaagatgt
    59101 ggtggagatg ccgggcttta aaccgagcca tgaagtgaaa gcgatgctcg acgacgcgga
    59161 tgtcttcctg ttgccatcgg ttacaggtgc ggatggcgat atggaaggca ttccggtggc
    59221 gctgatggag gcgatggcgg tcggcattcc ggtggtttct actctgcata gcggaatacc
    59281 ggaactggtg gaggctgaca aatccggctg gctggtgcct gagaacgatg ctcgcgcact
    59341 ggcgcaacga ctggcggcgt ttagccaact ggacaccgac gaattggctc cggtcgtcaa
    59401 acgcgcgcgc gaaaaagttg aacacgattt taaccagcag gtgatcaatc gagaactcgc
    59461 cagcttgctg caggctttat agaggagaac gtatgccatt taaaaaactc tcccgacgca
    59521 ccttcctgac ggcaagctcg gcgcttgcct tccttcatac ccctttcgct cgcgcacttc
    59581 ccgcccgaca aagcgttaat atcaacgact acaaccctca cgactggatc gcctcattta
    59641 aacaagcctt cagcgaaggg caaacggttg tcgtgcctgc tggattcgtt tgtgacaata
    59701 tcaacaccgg catcttcatt cctcctggca aaacgttaca catccttgga agcctgcgcg
    59761 gcaacggcag agggcgattt gtcttacagg acggcagcca ggtgacaggg gaggagggcg
    59821 gcagtatgca taacatcacc ctggatgtgc gtggttctga ctgcaccatc aaagggctgg
    59881 cgatgagcgg ctttggtccg gtaacgcaga tttatatcgg cggcaaaaac aaacgggtca
    59941 tgcgcaacct gaccatcgat aacctgacca ttagccacgc taattacgcc atcttacgcc
    60001 agggatttca taaccagatt atcggtgcca acatcaccaa ttgtaagttc agcgacttac
    60061 agggcgatgc cattgaatgg aacgtggcga ttaacgaccg cgatattttg atctccgacc
    60121 atgtcatcga gcgcatcaac tgtactaacg gcaaaatcaa ctggggcatt ggcataggcc
    60181 ttgcgggaag cacttacgat aataattacc cggaagatca ggcagtgaaa aactttgtcg
    60241 tggcgaatat cacgggatcg gattgtcggc agttgataca tgttgaaaat ggtaaacatt
    60301 ttgttattcg taatatcaaa gcccgcaata tcacgcagga tttcagtaag aaagcaggta
    60361 ttgataacgc cacagtcgct atttacggtt gtgacaattt cgtgattgat aatattgaaa
    60421 tgattaatag cgccgggatg ttaatcggct atggggtaat taaaggcaat tatctctcga
    60481 ttccgcaaaa tttccgagtg aataatattc aactggataa cacccatctt gcttataaat
    60541 tgcgcggcct ccaaatctct gccgggaatg ccgtctcctt tgtggcacta actaacattg
    60601 agatgaagcg tgcgtcgctg gagttacaca acaaaccgca acatcttttt ctgcgtaata
    60661 tcaaagtgat gcaggaatcc tctgttggac ccgcattgat tatgaacttc gacatgcgca
    60721 aagacgttcg aggcgtcttt atggcgaaag aagaaacact gctgtctctt gcaaatgttc
    60781 atgcggtgaa tgagaaagga caaagctccg tcgatatcga caggattaat caccatattg
    60841 ttaatgtgga aaagattaac tttagattgc cggaacggag agagtagatt tgcgaccatt
    60901 cataaactta ctggatataa tgttctaaaa gattataatc ctcacactaa ctgttaaagc
    60961 tggggctggg cagcattgac tactcaggga aggtactcat ctttatcttg tgtttcttac
    61021 gtaacaacca gactaaagtg atttatgaac gataacgttt tgctcatagg agcttccgga
    61081 ttcgtaggaa cccgactact tgaaacggca attgctgact ttaatatcaa gaacctggac
    61141 aaacagcaga gccactttta tccagaaatc acacagattg gtgatgttcg tgatcaacag
    61201 gcactcgacc aggcgttagc cggttttgac actgttgtac tactggcagc ggaacaccgc
    61261 gatgacgtca gccctacttc tctctattat gatgtcaacg ttcagggtac ccgcaatgtg
    61321 ctggcggcca tggaaaaaaa tggcgttaaa aatatcatct ttaccagttc cgttgctgtt
    61381 tatggtttga acaaacacaa ccctgacgaa aaccatccac acgacccttt caaccactac
    61441 ggcaaaagca agtggcaggc ggaggaagtg ctgcgtgaat ggtataacaa agcaccaaca
    61501 gaacgttcat taactatcat ccgtcctacc gttatcttcg gtgaacgcaa ccgcggtaac
    61561 gtctataact tgctgaaaca gatcgctggc ggcaagttta tgatggtggg cgcagggact
    61621 aactataagt ccatggctta tgttggaaac attgttgagt ttatcaagta caaactgaag
    61681 aatgttgccg caggttacga ggtttataac tacgttgata agccagacct gaacatgaac
    61741 cagttggttg ctgaagttga acaaagcctg aacaaaaaga tcccttctat gcacttgcct
    61801 tacccactag gaatgctggg tggatattgc tttgatatcc tgagcaaaat tacgggcaaa
    61861 aaatacgctg tcagctctgt gcgcgtgaaa aaattctgcg caacaacaca gtttgacgca
    61921 acgaaagtgc attcttcagg ttttgtggca ccgtatacgc tgtcgcaagg tctggatcga
    61981 actctgcagt atgaattcgt ccatgccaaa aaagacgaca taacgtttgt ttctgagtaa
    62041 tgcagacgag tcagagaaaa aaggaccagg ttggactacg attattcgga aagaggtact
    62101 gattaaatta tcagtatgtg gatttccgag taatccgagt aacccagatg ctgatggtgt
    62161 gtagtgttat cgtaaggttt aatcaaagtt atgcggaact aagctttggt atatagccat
    62221 aacgtttggc taacctatgg caataattta cgcactatca gccacttaga ggtaggaaaa
    62281 gaatgacgaa tttaaaagca gttataccgg tagcaggtct tgggatgcat atgttgcctg
    62341 ccactaaggc gattcccaaa gagatgctac cgatcgtcga caagccaatg attcagtaca
    62401 ttgttgacga gattgtggct gcagggatca aagaaatcct cctggtaact cacgcgtcca
    62461 agaacgcagt cgaaaaccac ttcgacacct cttatgaatt agaatctctc cttgaacagc
    62521 gcgtgaagcg tcaactactg gcggaagtac aatctatctg tccgccgggc gtgaccatta
    62581 tgaacgtgcg tcagggtgaa cctttaggtt taggccactc cattttatgt gcgcgacctg
    62641 ccattggtga caatccattt gtcgtggtgc tgccagacgt tgtgatcgac gacgccagcg
    62701 ccgacccgct acgctacaac cttgctgcca tgattgcgcg cttcaatgaa acgggacgca
    62761 gccaggtgct ggcaaaacgt atgccgggcg acctctctga atactccgtc atccagacca
    62821 aagaaccgct ggatcgtgaa ggcaaagtca gccgcattgt tgaatttatc gaaaagccgg
    62881 atcagccgca gacgctggac tcagacatca tggccgttgg tcgatatgtg ctttctgccg
    62941 atatttggcc ggaactggaa cgtactcagc ctggtgcatg gggacgtatt cagctgactg
    63001 atgccattgc tgagctggca aaaaaacaat ccgttgatgc aatgctgatg actggcgaca
    63061 gttacgactg cggtaaaaaa atgggctata tgcaggcgtt tgtgaagtat ggactacgca
    63121 acttgaaaga aggggcgaag ttccgcaaag gtatcgagaa gctgttaagc gaataatgaa
    63181 aatctgaccg aatgtaacgg ttgataagga aataatatcg gcagtgaaaa actatagatc
    63241 aataaatatc actgctgttg agcttttata attaactatg ctaccaatga ataaatagtg
    63301 tgcagatatg tattttttgt ttggtagtct tgtttctccc tctgaataat aagataataa
    63361 acgcttggag atgatttttt taacatggat tatcatttcg tacactggta gcgattaagc
    63421 caggggcggt agcgtgc
//
LOCUS       DAZFAB010000017        61220 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZFAB010000017 DAZFAB010000000
VERSION     DAZFAB010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 61220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61220
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.017"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..987
                     /gene="pgaA"
                     /locus_tag="ACHJWA_001235"
     CDS             <1..987
                     /gene="pgaA"
                     /locus_tag="ACHJWA_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:1901515 -
                     poly-beta-1,6-N-acetyl-D-glucosamine transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HFO4307878.1"
                     /translation="LTLQEWQQAAVLTHDVVEREPQDPGVVRLKRAVDVHNLAELRIA
                     GSTGIDAEGPDSGKHDVDLTTIVYSPPLKDNWRGFAGFGYADGQFSEGKGIVRDWLAG
                     VEWRSRNIWLEAEYAERVFNHEHKPGARLSGWYDFNDNWRIGSQLERLSHRVPLRAMK
                     NGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSLEGQERIWSSPYLIVDF
                     LPSLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLLWRSYENSWEQIFSAGVGASWQKH
                     YGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLYVEFDMTFRF"
     gene            996..3014
                     /gene="pgaB"
                     /locus_tag="ACHJWA_001236"
     CDS             996..3014
                     /gene="pgaB"
                     /locus_tag="ACHJWA_001236"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HFO4307879.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRKSLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADKQVKFGDELVD
                     REYFVTWQQVREVARSRLVELASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKVEVNPHVFIWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKSPQRIMHIDLDYVYDENLQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYHRLSPFDDRVRAQVGMLY
                     EDLAGHAAFDGILFHDDALLSDYEDASAPAITAYQQAGFSGSLSEIRQNPEQFKQWAR
                     FKSRALTDFTLELSARVKAIRGPHIKTARNIFALPVIQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYLEGVAEKSADQWLIQLTNQIKNIPQAKDKSILELQAQNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDLIRPEFSTAWYPKND"
     gene            3007..4332
                     /gene="pgaC"
                     /locus_tag="ACHJWA_001237"
     CDS             3007..4332
                     /gene="pgaC"
                     /locus_tag="ACHJWA_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008375 - acetylglucosaminyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042710 - biofilm formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine synthase"
                     /protein_id="HFO4307880.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIVGGVYFWVYRERRWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYSLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLT
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            4334..4747
                     /gene="pgaD"
                     /locus_tag="ACHJWA_001238"
     CDS             4334..4747
                     /gene="pgaD"
                     /locus_tag="ACHJWA_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HFO4307881.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(4797..5585)
                     /gene="phoH"
                     /locus_tag="ACHJWA_001239"
     CDS             complement(4797..5585)
                     /gene="phoH"
                     /locus_tag="ACHJWA_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFO4307882.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(6205..7476)
                     /gene="efeB"
                     /locus_tag="ACHJWA_001240"
     CDS             complement(6205..7476)
                     /gene="efeB"
                     /locus_tag="ACHJWA_001240"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFO4307883.1"
                     /translation="MQYEDKNGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPVARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKGIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(7482..8609)
                     /gene="efeO"
                     /locus_tag="ACHJWA_001241"
     CDS             complement(7482..8609)
                     /gene="efeO"
                     /locus_tag="ACHJWA_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFO4307884.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(8667..9497)
                     /gene="efeU"
                     /locus_tag="ACHJWA_001242"
     CDS             complement(8667..9497)
                     /gene="efeU"
                     /locus_tag="ACHJWA_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFO4307885.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            10679..12022
                     /locus_tag="ACHJWA_001243"
     CDS             10679..12022
                     /locus_tag="ACHJWA_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4307886.1"
                     /translation="MRPISNLNTSAATYIPPQQLPSSCLETLTLLVQMNNYIKRDADY
                     STGMAVAPLVPEEVHLLAAAMTTELHRHQFQPVVSANDLQLPEPFTFDVAGFTITFTK
                     TQEHDNTGNLMKIAVSKSGVCTSTNITLELFHSIVTTLMSRSQYGTFDLWSVRPILTD
                     ESQNRVHEAARYSPAQQYGREETHFTNNGMREFGNMSPLAWRNDVELQQSCNTSNIPP
                     NAANDDINNTRQTIEDQPEADEPQQYVKLTVDDMRKWAAMDQQARNALQGVSGWCTRN
                     HFNIKNARNYLTDHGLNYAGQVKVNRPHEYAKFTLEHIRQWAALNKHVRKPVGYLEKW
                     CKERNLAPTTARNYLKNDGLTALGELKLTGPQKWVTFTFGDIVQWANMTQEERNSAGG
                     AEKWSKKRGFQWSTARSYLKSSGVTKQGARKLAWLKNSGNMSNPFYLAQPTSRRT"
     gene            complement(12171..13679)
                     /gene="putP"
                     /locus_tag="ACHJWA_001244"
     CDS             complement(12171..13679)
                     /gene="putP"
                     /locus_tag="ACHJWA_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFO4307887.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPSVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEG"
     gene            complement(13838..14047)
                     /locus_tag="ACHJWA_001245"
     CDS             complement(13838..14047)
                     /locus_tag="ACHJWA_001245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4307888.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            14102..18064
                     /gene="putA"
                     /locus_tag="ACHJWA_001246"
     CDS             14102..18064
                     /gene="putA"
                     /locus_tag="ACHJWA_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO4307889.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGYVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGRGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVIDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSTVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(18104..18742)
                     /gene="rutR"
                     /locus_tag="ACHJWA_001247"
     CDS             complement(18104..18742)
                     /gene="rutR"
                     /locus_tag="ACHJWA_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFO4307890.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKSLIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            19030..20121
                     /gene="rutA"
                     /locus_tag="ACHJWA_001248"
     CDS             19030..20121
                     /gene="rutA"
                     /locus_tag="ACHJWA_001248"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFO4307891.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPVLTQEVA"
     gene            20121..20813
                     /gene="rutB"
                     /locus_tag="ACHJWA_001249"
     CDS             20121..20813
                     /gene="rutB"
                     /locus_tag="ACHJWA_001249"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFO4307892.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTS
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFVQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            20825..21211
                     /gene="rutC"
                     /locus_tag="ACHJWA_001250"
     CDS             20825..21211
                     /gene="rutC"
                     /locus_tag="ACHJWA_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFO4307893.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINETYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            21219..22019
                     /gene="rutD"
                     /locus_tag="ACHJWA_001251"
     CDS             21219..22019
                     /gene="rutD"
                     /locus_tag="ACHJWA_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFO4307894.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSITQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDHP
                     ASVTVLVCVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            22029..22619
                     /gene="rutE"
                     /locus_tag="ACHJWA_001252"
     CDS             22029..22619
                     /gene="rutE"
                     /locus_tag="ACHJWA_001252"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFO4307895.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIIACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            22630..23124
                     /gene="rutF"
                     /locus_tag="ACHJWA_001253"
     CDS             22630..23124
                     /gene="rutF"
                     /locus_tag="ACHJWA_001253"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFO4307896.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            23145..24473
                     /gene="rutG"
                     /locus_tag="ACHJWA_001254"
     CDS             23145..24473
                     /gene="rutG"
                     /locus_tag="ACHJWA_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFO4307897.1"
                     /translation="MAMFGLPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASGFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGSSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(24556..24663)
                     /locus_tag="ACHJWA_001255"
     CDS             complement(24556..24663)
                     /locus_tag="ACHJWA_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HFO4307898.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            25035..25631
                     /gene="wrbA"
                     /locus_tag="ACHJWA_001256"
     CDS             25035..25631
                     /gene="wrbA"
                     /locus_tag="ACHJWA_001256"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO4307899.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMS
                     PQLFEKAGGKTQTAPVATPQELANYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            25652..25879
                     /gene="yccJ"
                     /locus_tag="ACHJWA_001257"
     CDS             25652..25879
                     /gene="yccJ"
                     /locus_tag="ACHJWA_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFO4307900.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(25917..27158)
                     /gene="agp"
                     /locus_tag="ACHJWA_001258"
     CDS             complement(25917..27158)
                     /gene="agp"
                     /locus_tag="ACHJWA_001258"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFO4307901.1"
                     /translation="MNKTLIAAAVAGVVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWRDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDADGFCPMDKFDSVLNEAVK"
     gene            complement(27449..28706)
                     /locus_tag="ACHJWA_001259"
                     /pseudo
     CDS             complement(27449..28706)
                     /locus_tag="ACHJWA_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
     gene            28966..29886
                     /gene="cbpA"
                     /locus_tag="ACHJWA_001260"
     CDS             28966..29886
                     /gene="cbpA"
                     /locus_tag="ACHJWA_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFO4307902.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFSRQFQHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            29886..30191
                     /gene="cbpM"
                     /locus_tag="ACHJWA_001261"
     CDS             29886..30191
                     /gene="cbpM"
                     /locus_tag="ACHJWA_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFO4307903.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(30345..30944)
                     /gene="torD"
                     /locus_tag="ACHJWA_001262"
     CDS             complement(30345..30944)
                     /gene="torD"
                     /locus_tag="ACHJWA_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFO4307904.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVDELENRVATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPTDHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LRQWLPEFVARCHQYDSFGFYAALSQLLLVLVEGDHQNR"
     gene            complement(30941..33487)
                     /gene="torA"
                     /locus_tag="ACHJWA_001263"
     CDS             complement(30941..33487)
                     /gene="torA"
                     /locus_tag="ACHJWA_001263"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HFO4307905.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTSRRATAAQAAT
                     EAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQTYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWHDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKCNGTVEQVTAFNGPVEMVAQCEYVPASQVKL"
     gene            complement(33487..34659)
                     /gene="torC"
                     /locus_tag="ACHJWA_001264"
     CDS             complement(33487..34659)
                     /gene="torC"
                     /locus_tag="ACHJWA_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFO4307906.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRAGANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEISHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            34789..35481
                     /gene="torR"
                     /locus_tag="ACHJWA_001265"
     CDS             34789..35481
                     /gene="torR"
                     /locus_tag="ACHJWA_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFO4307907.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPYTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            complement(35454..36482)
                     /gene="torT"
                     /locus_tag="ACHJWA_001266"
     CDS             complement(35454..36482)
                     /gene="torT"
                     /locus_tag="ACHJWA_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFO4307908.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            36565..39309
                     /gene="torS"
                     /locus_tag="ACHJWA_001267"
     CDS             36565..39309
                     /gene="torS"
                     /locus_tag="ACHJWA_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HFO4307909.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQQRLSQQIVAAADEIARLAQGQAN
                     NAATSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALF
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDVPTALMGDPRRIRQVI
                     TNLLSNALRFTDEGQIVLRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFIQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAIGNAAQALETLQNSEPFAAALVDFDLPDVN
                     GITLARQLARQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQANNDLPLDVSQLNEDAQLMGTEKIHEWLALFTQHALPLLDEIDIARATQDSEKIK
                     RAAHQLKSSCSSLGMRSASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLNKKDLNAI
                     "
     gene            39381..40454
                     /gene="yccM"
                     /locus_tag="ACHJWA_001268"
     CDS             39381..40454
                     /gene="yccM"
                     /locus_tag="ACHJWA_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFO4307910.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWHNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETAGSTIFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGALGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFTVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(40503..40637)
                     /locus_tag="ACHJWA_001269"
     CDS             complement(40503..40637)
                     /locus_tag="ACHJWA_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFO4307911.1"
                     /translation="MADFIAQKIAELNKNTGKEVSEIRFTAREKMTGLESYDVKIKIM
                     "
     gene            complement(40665..40895)
                     /gene="ymcE"
                     /locus_tag="ACHJWA_001270"
     CDS             complement(40665..40895)
                     /gene="ymcE"
                     /locus_tag="ACHJWA_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFO4307912.1"
                     /translation="MHRWISQNNIRLPCGAFFISVLFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            complement(40870..41058)
                     /gene="ymcF"
                     /locus_tag="ACHJWA_001271"
     CDS             complement(40870..41058)
                     /gene="ymcF"
                     /locus_tag="ACHJWA_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFO4307913.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            complement(41069..41281)
                     /gene="cspG"
                     /locus_tag="ACHJWA_001272"
     CDS             complement(41069..41281)
                     /gene="cspG"
                     /locus_tag="ACHJWA_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFO4307914.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            41567..41779
                     /gene="cspH"
                     /locus_tag="ACHJWA_001273"
     CDS             41567..41779
                     /gene="cspH"
                     /locus_tag="ACHJWA_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFO4307915.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            42221..42526
                     /locus_tag="ACHJWA_001274"
     CDS             42221..42526
                     /locus_tag="ACHJWA_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4307916.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            42633..43277
                     /gene="gfcB"
                     /locus_tag="ACHJWA_001275"
     CDS             42633..43277
                     /gene="gfcB"
                     /locus_tag="ACHJWA_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFO4307917.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLHIAGRDV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            43274..44020
                     /gene="gfcC"
                     /locus_tag="ACHJWA_001276"
     CDS             43274..44020
                     /gene="gfcC"
                     /locus_tag="ACHJWA_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFO4307918.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPVEN
                     VAQLVTQPQLRDRLWWPGALLTDSAVKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGNYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVIVITPEGETVVAPVALWNKRHVEPPLGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            44020..46116
                     /gene="gfcD"
                     /locus_tag="ACHJWA_001277"
     CDS             44020..46116
                     /gene="gfcD"
                     /locus_tag="ACHJWA_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO4307919.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEPLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            46162..47301
                     /gene="gfcE"
                     /locus_tag="ACHJWA_001278"
     CDS             46162..47301
                     /gene="gfcE"
                     /locus_tag="ACHJWA_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFO4307920.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            47289..47735
                     /gene="etp"
                     /locus_tag="ACHJWA_001279"
                     /gene_synonym="yccY"
     CDS             47289..47735
                     /gene="etp"
                     /locus_tag="ACHJWA_001279"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFO4307921.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            47755..49935
                     /gene="etk"
                     /locus_tag="ACHJWA_001280"
     CDS             47755..49935
                     /gene="etk"
                     /locus_tag="ACHJWA_001280"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFO4307922.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDMVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(50055..51359)
                     /gene="appA"
                     /locus_tag="ACHJWA_001281"
     CDS             complement(50055..51359)
                     /gene="appA"
                     /locus_tag="ACHJWA_001281"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFO4307923.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPEPELKLESVVIVSRHGVR
                     APTKATQLMQDVTPDAWPNWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLTKKGCP
                     QPGQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLD
                     NANVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQAL
                     PSELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNA
                     QFYLLQRTPEVARSRATPLLDLIMIALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANL
                     GGALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSL
                     NTPPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(51439..51531)
                     /gene="cbdX"
                     /locus_tag="ACHJWA_001282"
     CDS             complement(51439..51531)
                     /gene="cbdX"
                     /locus_tag="ACHJWA_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFO4307924.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            complement(51544..52680)
                     /gene="appB"
                     /locus_tag="ACHJWA_001283"
     CDS             complement(51544..52680)
                     /gene="appB"
                     /locus_tag="ACHJWA_001283"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO4307925.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARDDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLSTKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            complement(52692..54236)
                     /gene="appC"
                     /locus_tag="ACHJWA_001284"
     CDS             complement(52692..54236)
                     /gene="appC"
                     /locus_tag="ACHJWA_001284"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFO4307926.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPVLLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            complement(54370..55227)
                     /gene="hyaF"
                     /locus_tag="ACHJWA_001285"
     CDS             complement(54370..55227)
                     /gene="hyaF"
                     /locus_tag="ACHJWA_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFO4307927.1"
                     /translation="MSETFFHLLGPRTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSQRQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            complement(55224..55622)
                     /gene="hyaE"
                     /locus_tag="ACHJWA_001286"
     CDS             complement(55224..55622)
                     /gene="hyaE"
                     /locus_tag="ACHJWA_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFO4307928.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSEFRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            complement(55619..56206)
                     /gene="hyaD"
                     /locus_tag="ACHJWA_001287"
     CDS             complement(55619..56206)
                     /gene="hyaD"
                     /locus_tag="ACHJWA_001287"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFO4307929.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPESVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG"
     gene            complement(56203..56910)
                     /gene="hyaC"
                     /locus_tag="ACHJWA_001288"
     CDS             complement(56203..56910)
                     /gene="hyaC"
                     /locus_tag="ACHJWA_001288"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFO4307930.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            complement(56929..58722)
                     /gene="hyaB"
                     /locus_tag="ACHJWA_001289"
     CDS             complement(56929..58722)
                     /gene="hyaB"
                     /locus_tag="ACHJWA_001289"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFO4307931.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFNKLMTNLKNGNLATASTEKWEPTTWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            complement(58719..59837)
                     /gene="hyaA"
                     /locus_tag="ACHJWA_001290"
     CDS             complement(58719..59837)
                     /gene="hyaA"
                     /locus_tag="ACHJWA_001290"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFO4307932.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATSIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            60263..60350
                     /locus_tag="ACHJWA_001291"
     tRNA            60263..60350
                     /locus_tag="ACHJWA_001291"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:60297..60299,aa:Ser,seq:tga)
     gene            60953..61220
                     /gene="tccP2"
                     /locus_tag="ACHJWA_001292"
                     /pseudo
     CDS             60953..61220
                     /gene="tccP2"
                     /locus_tag="ACHJWA_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_107686092.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tir-cytoskeleton coupling protein TccP2"
BASE COUNT        15177 a        15914 c        15372 g        14757 t
ORIGIN      
        1 ttaacgttac aagaatggca gcaggcagct gtcttaacgc acgatgttgt cgaacgtgaa
       61 ccgcaagatc ccggcgttgt acgattaaaa cgtgcggttg atgtacataa tcttgcagag
      121 cttcgtatcg ctggctcaac aggaattgat gccgaaggcc cggatagtgg taaacatgat
      181 gtcgacttaa ccaccatcgt ttattcacca ccgctgaagg ataactggcg cggttttgct
      241 ggattcggtt atgccgatgg acaatttagc gaaggaaaag ggattgttcg cgactggctt
      301 gcgggtgttg agtggcggtc acgtaatatc tggctcgagg cagagtacgc tgaacgcgtt
      361 ttcaatcatg agcataaacc cggcgcgcgc ctgtctggct ggtatgattt taatgataac
      421 tggcgtattg gttcgcaact ggaacgcctc tctcaccgcg ttccattacg ggcaatgaaa
      481 aatggtgtta caggcaacag tgctcaggct tatgttcgct ggtatcaaaa tgagcggcgt
      541 aagtacggtg tctcctgggc tttcactgat ttttccgaca gtaaccagcg tcatgaagtc
      601 tcacttgagg gtcaggaacg catctggtct tcaccatatt tgattgtcga tttcctaccc
      661 agtctgtatt acgaacaaaa tacagaacac gataccccat actacaaccc tataaaaacg
      721 ttcgatattg ttccggcatt tgaggcaagc catttgttat ggcgaagcta tgaaaatagc
      781 tgggagcaaa tattcagcgc aggtgttggt gcctcctggc aaaaacatta tggcacggat
      841 gtcgtcaccc aactcggcta cgggcaacgc attagctgga atgacgtgat tgatgctggc
      901 gcaacgctac gctgggaaaa acgaccttat gacggtgaca gagaacacaa cttatacgtt
      961 gaattcgata tgacattcag attttaagga taaatatgtt acgtaatgga aataaatatc
     1021 tcctgatgct ggtgagtata attatgctca ccgcgtgcat tagccagtca agaacatcat
     1081 ttataccgcc acaggatcgc aaatctttac tcgccgagca accgtggccg cataatggtt
     1141 ttgtagcgat ttcatggcat aacgttgaag acgaagctgc cgaccagcgt tttatgtcag
     1201 tgcggacatc agcactgcgt gaacaatttg cctggctgcg cgagaacggt tatcaaccgg
     1261 tcagtattgc tcaaattcgt gaagcacatc gaggaggaaa accgctaccg gaaaaagctg
     1321 tagtgctgac ttttgatgac ggctaccaga gtttttatac ccgcgtcttc ccaattcttc
     1381 aggccttcca gtggcctgct gtatgggccc ccgtcggcag ttgggtcgat acgccagcgg
     1441 ataaacaagt aaaatttggc gatgagttgg tcgatcgaga atattttgtc acgtggcaac
     1501 aagtgcgaga agttgcgcgt tcccggctcg ttgagctcgc ttctcataca tggaattctc
     1561 actacggtat tcaggctaat gccaccggca gcttattgcc tgtatatgta aatcgtgcat
     1621 attttactga ccacgcacgg tatgaaaccg cagcagaata ccgggaaaga attcgtctgg
     1681 atgctgtaaa aatgacggaa tacctgcgta caaaggttga ggtaaatcca cacgttttta
     1741 tttggcctta tggcgaagcg aatggcatag cgatagagga attaaaaaaa ctcggttatg
     1801 acatgttctt cacccttgaa tcaggtttgg caaatgcgtc gcaattggat tccattccgc
     1861 gggtattaat cgccaataat ccctcattaa aagagtttgc ccagcaaatt attaccgtac
     1921 aggaaaaatc accacaacgg ataatgcata tcgatcttga ttacgtttat gacgaaaacc
     1981 tccagcaaat ggatcgcaat attgatgtgc taattcagcg ggtgaaagat atgcaaatat
     2041 caaccgtgta tttgcaggca tttgctgatc ccgatggtga tgggctggtc aaagaggtct
     2101 ggtttccaaa tcgtttgcta ccaatgaaag cagatatttt tagtcgggtt gcctggcaat
     2161 tacgtacccg ctcaggtgta aacatctatg cgtggatgcc ggtattaagc tgggatttag
     2221 atcccacatt aacgcgagta aaatacttac caacagggga gaaaaaagca caaattcatc
     2281 ctgaacaata tcaccgtctc tctcctttcg atgacagagt cagagcacaa gttggcatgt
     2341 tatatgaaga tcttgccgga catgctgctt ttgatggcat attgttccac gatgatgctt
     2401 tgctttcaga ttatgaagat gccagtgcac cggctatcac ggcttatcag caagcaggct
     2461 ttagcgggag tctgagcgaa attcgacaaa acccggagca atttaaacag tgggcccgct
     2521 ttaaaagtcg tgcgttaact gacttcactt tagaacttag tgcgcgcgta aaagccattc
     2581 gcggtccaca tattaaaact gcacgaaata tttttgcact tccggtaata caacctgaaa
     2641 gtgaagcctg gtttgcacag aattatgctg atttcctaaa aagctatgac tggaccgcta
     2701 ttatggctat gccttatctg gaaggtgtcg cagaaaaatc ggctgaccaa tggttaatac
     2761 aattgaccaa tcaaattaaa aacatccctc aggctaaaga caaatctatt ttagaattac
     2821 aggcacaaaa ctggcagaaa aatggtcagc atcaggctat ttcttcgcaa caactcgctc
     2881 actggatgag cctattacaa ctgaatggag tgaaaaacta tggttattat cccgacaatt
     2941 ttctgcataa ccaacctgaa atagacctta ttcgtcctga gttttcaaca gcctggtatc
     3001 cgaaaaatga ttaatcgcat cgtatcgttt tttatattat gtctggtgtt atgcataccc
     3061 ctatgcgtag cgtactttca ctctggtgaa ctgatgatga ggttcgtttt cttctggccg
     3121 ttttttatgt ccattatgtg gattgttggc ggcgtctatt tctgggtcta tcgtgaacgc
     3181 cgctggccgt ggggagaaaa cgcaccagct ccccagttga aagataatcc gtctatctcc
     3241 attatcattc cctgttttaa tgaggagaaa aacgttgagg aaaccataca cgccgcttta
     3301 gcacagcgtt atgagaacat tgaagttatt gccgtaaatg acggttcaac agataaaacc
     3361 cgtgccatcc tggatcgcat ggctgcacaa attccccatt tgcgggtcat tcatctggcg
     3421 caaaaccagg ggaaagccat tgcgcttaaa accggagctg ccgcggcgaa aagtgaatat
     3481 ctggtgtgca ttgatggcga tgcgttatta gaccgcgatg cggcggcata tattgtggaa
     3541 ccgatgttgt acaacccgcg tgtgggtgcc gtaaccggta atcctcgtat tcgaacacgt
     3601 tctaccctgg tgggtaaaat tcaggttggc gagtattcct caattattgg tttgatcaag
     3661 cgaacccagc gtatctatgg aaacgtattt accgtttccg gtgttattgc cgcatttcgt
     3721 cgcagcgccc tggcagaagt gggttactgg agtgacgata tgatcaccga agatattgat
     3781 attagctgga agctgcagtt gaatcagtgg acgatttttt acgagccacg ggcactgtgc
     3841 tggatattaa tgcctgaaac gttaaaaggg ctgtggaaac agcgcctgcg ctgggctcag
     3901 ggcggtgcag aagtattcct caaaaatatg acaaggttgt ggcgcaaaga aaactttcga
     3961 atgtggccgc tgttttttga atacagcctg acgacaatat gggccttcac ctgcctggtc
     4021 ggtttcatta tttacgcagt ccaacttgcc ggtgtaccgt taaatattga attgacacat
     4081 atcgctgcga cacatactgc cggaatatta ttgtgtacgt tatgtttact gcaatttatt
     4141 gtcagcctga tgatcgagaa tcgctatgag cataatctga cttcatcgct tttctggatt
     4201 atttggttcc cggttatttt ctggatgctg agcctggcaa cgacattggt atcatttaca
     4261 cgagtcatgt tgatgcctaa aaagcaacgc gcccgttggg taagtcccga tcgcgggatt
     4321 ctgagaggtt aatatgaaca atttaattat tacgacccga caatcaccag tacgtttact
     4381 ggttgattat gttgccacaa ccatcttgtg gacattattt gcgttgttca tattcttatt
     4441 cgccatggat ctgctgacgg gttattactg gcaaagcgag gccagaagcc gacttcagtt
     4501 ctattttttg ctggcagtgg cgaatgccgt cgtgttaatt gtctgggcgc tgtacaataa
     4561 gctgcgtttt caaaaacagc agcatcatgc agcctaccaa tatacgccgc aagaatatgc
     4621 agagagctta gcaatacctg atgagctcta tcagcaacta caaaaaagcc acaggatgag
     4681 cgtacacttc accagccagg ggcaaataaa aatggttgtt tcagaaaaag cgctagtcag
     4741 ggcataaaca cccaaaacaa agcccggttc gcccgggctc tgcaccgata atacacttaa
     4801 ctgtatgcat gcagcgtacg ttggcaaagt gccgaacgta cgcaatcctc ttttccgaac
     4861 cggacgatcc caaccatttc atcttcttcg aaacgttcca gcgcgtcact taatccggag
     4921 cacacgccgc gaggcaaatc gcattgcgtg atatcaccgt tgacgataac cgtcacgttc
     4981 tccccgaggc gggttaaaaa cattttcatt tgcgcggcag tcacattctg cgcctcgtca
     5041 agaatgacga ctgcattttc aaaggtacgt ccacgcatat aggcgaacgg cgcaatttcc
     5101 accttcccta tttccggtcg caggcagtac tgcataaagg aagcccctaa gcgccggacc
     5161 agcacgtcgt agaccgggcg aaaataggga gcaaactttt ctgcgatatc tccaggtaag
     5221 aagccaagat cttcatcggc ttgcagaact ggacgggtga cgataatcct gtcgacatcc
     5281 ttatgtatca gggcctctgc cgcttttgct gcgctgatcc aggtttttcc gcacccggct
     5341 tcgcccgtgg cgaatatcag ctgcttactc tcaatagcct tcagatagtg caattgcgct
     5401 tcatttcgcg cgaggatggg cgaagtatcg cgactgtcgc gggccatacc aatggcttct
     5461 acgccgccca tctgcacaag cgaggtgacc gattcttctt cacgctgctt atggctgcgc
     5521 gaatcccgtc tcagcacacg ttttgcctcg cgacgagctt tgatcactgc tttttgtctt
     5581 cccatggaga gcaccttgag ttgtttgtat tcatcacacg cgccgttggc agcgcgatta
     5641 tgcgcacgaa catcagaggg ttggcttcct tgtaagccat agtttgcttt tggataaaat
     5701 gccgaaaaac ggctacgcgc accgtttacg gcgtcggtaa cacatgaaaa gaaaggatga
     5761 ggttgaaaat gcaaagtgac gagatgacta ccggaggaga aaactccgcg agtggtggcg
     5821 cgttgattat ctaaaacatg tccagtacag gacgttacca tccgcgatct ccatagtgac
     5881 tgactatcac tgccgggaac ttccgctgct acttaataag tacaacagtt ctcgcattta
     5941 ttgcaacaat atatttactt atatttaact ataaaacacc atttcagtga cattagtttc
     6001 tactggaaag atgacagagt gatgacagat atgaaaaaag ctgtgtgctt tcagcaggat
     6061 ttgcatcagg catcagtgca cgatcgccgt atgcggcgtg aacgccttat ccgcctaccg
     6121 aacccgcacc gattgtaggc ataagaagac gcgcaagcat cgcatcaggc atctgcgcaa
     6181 gaacgcctta tccgtctaaa aacattaaac ccgcaataac gcgcttccga gataatcgtt
     6241 cgcgtccttc actcccggca gcgcaaaaaa ataaccgccg ccgataggtt taacgtattc
     6301 ctccagcgct tcgccattga gccttttttg tactgtcagg aagccttttt ccagatcgtg
     6361 ttggtaacag acaaacagta gccccatatc cagttgcccg gagttggtga cgcccagtga
     6421 ataactgtag ccacgacgca gcatcaggct ggactcactc tccgccgtgc ggggattcgc
     6481 cagccggata tggctgtcca gcgcaatccc cttcccttcc gggtcgctgg cgtaatcagg
     6541 cacatcatgc tcatgctgca ttcccagcgg cgcaccggtt tgcttatcac ggccaaaaat
     6601 cgtctgctgt tctttcagcg gcgttctgtc ccaaaattcc actcgaaact gaatcaagcg
     6661 tactgcctga tagctgccac cgattgtcca cgcaggctcc tgctgatctg ccgttaccca
     6721 cacaactttt tgcatcaact tatcattctg gctatcggga ttggcagtgc cgtctttgaa
     6781 acccagcaaa ttaatcggcg tctctttgcc tttactacgc gccgcgtgat cggaaataaa
     6841 cccttcccgc ttccagcgca cgctgagcaa atccggcgta tgtttgatga tatcgcgcag
     6901 tgcatggatc accgtgtcct gggtgttggc gcaaatttgt agcaacacat caccatgaca
     6961 taacgccgca tccagcgagt cgttgggaaa acgcgtcatc ttctgcaact ttttcggcat
     7021 ctgtggcgca aggccaaagc gctcatcaaa caatgagtga cccaccgata acgtgatggt
     7081 gagattatcg ggcgcaatgt agccgccaag aatgccggaa tcgagtggtg gcaggcgcgg
     7141 atttggcgtt tctggtgccg ctccgccctg agtcagaaaa gcaaaacgct gagtcaacaa
     7201 gcgaaacaac cgttcaagat cggctttatc gctggcaagc acatcaaacg ccaccagcat
     7261 cattgcggcc tgttgtggcg tcaggatccc tgcctgatgc tcaccataaa acggctgttt
     7321 ctcattgcgt gcaaccggtg aaagcgtacc cggcgcactt tgcgtttttt gtgcatgagc
     7381 gaccggacaa cttcccgcca gcgccagcgc accaatgcct ttcagtaaac gtcggcgtga
     7441 cggttcattc acgccgtttt tatcttcata ctgcataatg cttaatccaa tcccagcaca
     7501 ccgcgaagtt gcgccagatc ttccgccagc gcagtaatcg gtcctttcag tgcattccgg
     7561 tcggcatcgg tcaatttgtc gtaggtttca aaaccgtctt tagtacggta tttcgccaga
     7621 atggtatcga cctttttaaa gttggcatcg acttttgcca acagttccgg gttagctttt
     7681 tgcagttgtg gacgcagcag atcgacaatt ttctgcgagc cttcaacgtt agcctggaaa
     7741 tcccacagat cggtgtggct gtagcgatct tcttcaccgc tgattttgct ggctgccact
     7801 tcctcaatca gtccggctgc accgccgacc acttttgaag gtgggaaagc cagttcactg
     7861 atgcgttttt gcaaatcgac cacatcggta taaagctggt cagcgtactg atccatgcct
     7921 ttggtggtgt tgtcgccaaa caatgctttt tccagacggt ggaaaccggt gaattttgga
     7981 tcagcggctt tttgctcgta atcatcttca cgggcgtcaa tgctgccatc cagatcggag
     8041 aacagttcag caatcggttc aatgcgctca tagtgctggc gcgtcggcgc atacagtgct
     8101 ttcgcttttt cgatatcgcc tgctttaatc gcgtcggtaa aggctttggt gtcggtcacc
     8161 agctgtgtgg tttccgccat gacatacgct ttatatgcag taattgcacc accaagactt
     8221 aacagcgcat cactttgcgc cgcatccgcc gttgcctcac ctttgacgat caacttccct
     8281 ttcgggttag tcagcagacc gcaggtcata tcgtattcgc caggctgtaa attcgccgtc
     8341 attttctggc taaagccagg ggcgatattt tcccgctctt ccaccaccat cacgcctttg
     8401 aggatctccc attccagcgc cttctggctg tggttctgaa taatgaactg tgttttcccg
     8461 gcgttaaccg taatggtcat cggttcgcac tgcttatccg tcacggtcac tttgacctgc
     8521 ggcacatcag cggcgttagc cataaaagca gaagaaaaca gcgcagccac gctcaactgc
     8581 aatgcgttac ggcggaagtt aatggtcatg acgagactat ccctttaaag tatgttgtaa
     8641 ctaagcaaga gtttgcgtcg tatttgttac gcggagcgag acgctgtcgc ccctgcgcgt
     8701 ggtggcagag caaatgccac cagcgccggg atgagataaa taaaccagac ggcgacttcg
     8761 ctgacgctcg gcgcttcctg atagccaaaa atcccttcca tcagcgtgcc aaacagcgag
     8821 tgagttgaga gcaccgcact catatcgaag gcgatttcct gaaagtggtt ccacaatccg
     8881 gcttcatgaa atgcgcgaat ggcaccagct gccagccctg cggcgacgaa gagaataaac
     8941 aggctggtcc atttaaaaaa tgcaccaaga ttgaggcgaa taccgcccca gtagagcagg
     9001 aagcctagca ccacggcagt agcaagaccg agcattgcac ccagcggcgg ccagatcccg
     9061 acatcttgtt gaaatgccgc cagcaggaaa aagaccgact ccagcccttc ccttgcaacg
     9121 gcaaaaaaga ccatcatcac cagcgcccag ccatgatgat ttccacgctg caatgcgcta
     9181 tcgacagcct gctccagttg tactttgacg ttgcgcgaca ctttgcgcat ccagaaaacc
     9241 atccaggtaa ggatcaccac ggcgatcacc gccacgatac cttcaaacag ttcctgctct
     9301 ttttgcggaa attcgccggt ggtttcgtta atgaagatcc ccaagcccag gcacaacgca
     9361 gcggcaagca acacgccaat ccacatcaca ccaatccatc ggcctcgctg ggtacgctta
     9421 agatagctgg caatcaaact gacaatcagc gcggcttcaa gtccttcgcg caacataatg
     9481 agaaacggaa caaacatgcc acccaccctt aaacgttttt ctgagtcaat ctatgcaaag
     9541 aaaagtaaat cgcagtgata gcgattatca ttacgctaaa gaaaaataca agcggaatat
     9601 ttataaaatg atgttggaat gtaaaaatga ggagatcaaa tccggagaaa aaagcgatta
     9661 ttttaaggta tttagctgac gtttcgcccg gcagatgaag cttgtacgaa tgttggtggc
     9721 gacaaactcg ctgcagcgtg cccgaagggt tgaattttca gttagtcgtt tcacaaaaga
     9781 acgttgtttt ttgcttatta atagatagtg ataaggttgt gaatgaaatt atacctcatc
     9841 tcctcatcga attttttgcc cacaacctaa acaggagaaa acgtgtattt aagtctggaa
     9901 tgagcgattt ttgatagtgc ctaattatcc cccaaaatta taattcccct tattatattc
     9961 ccagtgatat cattttatgg ctaatgctac atacaattct aaatcacgat aaaacgaagc
    10021 aggatgcgca ttaataaagt cacacataat aaaaatagaa atcttaacca gaagtaatag
    10081 atcatcgtga aaatcaatca ccaaaaacac caacacacaa aaatcaataa tttaataatt
    10141 gttcagggct tttttctcac gatttttcta ctatgacatt atcacaaacc cactagcatg
    10201 aatacagaaa gagaagtaat aagatgagac gccactgctt tcagatgaac cctgtaatta
    10261 gggtcgagaa tataatttac ccattcccct aacgataaat taaaaaattt gcgagttaat
    10321 ttttctctgg ctgaaggcta acgtcgagtt atcgataaaa ttcacgagta attgtaacta
    10381 tatactttat gaaacacagg aagaaattat tatttaacgc gatgataaaa ggcgtaaaaa
    10441 aaaataataa tcttcatgaa actcaccccg atggcagcat tgtaaattcg ccaataactg
    10501 gcattcctcc tgctttttac gggtgtttcc attaacacca gaatgagcgg attgtttact
    10561 acatcaagaa cgcatccgaa gtgtacatcc tggtgcgccg actgaatcgc ccggagccat
    10621 gcggatggct acgacttaat acgaatcatt aaccctgtaa ggatacggag agcattacat
    10681 gagaccaata tccaacctca atacgagcgc agcaacctat attccaccgc aacaactccc
    10741 gagtagttgt ctggaaactt tgacgcttct ggtgcagatg aataattata tcaaaaggga
    10801 tgcggactac agcacgggca tggctgtagc tccactggtt cctgaagagg ttcacttgct
    10861 ggctgctgcg atgacaaccg aactgcatcg acatcagttc caaccggtgg taagtgcaaa
    10921 tgatctgcaa cttcctgaac cgttcacctt cgatgttgcc ggtttcacca tcaccttcac
    10981 gaagacgcaa gagcatgaca acacgggcaa tctcatgaaa attgcagtca gcaagagcgg
    11041 tgtgtgcacc agtacaaata ttactcttga gctgtttcac tccattgtca caaccctgat
    11101 gtcacgcagt caatatggaa cctttgatct ctggtccgta cggccaatac tcacggatga
    11161 aagtcaaaac agggtgcatg aagcagctcg ctactccccg gcccagcaat acggaagaga
    11221 agaaacacac tttaccaaca atggcatgcg agaatttgga aatatgtctc cgctggcgtg
    11281 gcgtaacgat gtggaattac agcaatcgtg taatacaagc aacatcccac caaatgctgc
    11341 aaatgacgac ataaacaaca ccaggcagac catcgaggac cagccagagg cggacgagcc
    11401 acagcaatat gttaaattga ctgtagacga catgcgaaaa tgggcggcta tggaccagca
    11461 ggcgcgaaac gctctacagg gagtttcagg atggtgtacg cgcaatcatt ttaatatcaa
    11521 aaatgccaga aattacctga ccgatcacgg cctgaactac gcgggacagg taaaggtaaa
    11581 caggccgcat gaatatgcca aattcaccct tgaacatatc cgccagtggg cagccttaaa
    11641 caagcatgtg cgaaaaccgg ttggttattt agaaaagtgg tgtaaggaaa gaaacctcgc
    11701 gccaactact gccaggaatt acctgaagaa cgacgggctt accgccctgg gtgagttgaa
    11761 gctgacaggg ccgcagaaat gggtcacgtt caccttcgga gacatcgtac aatgggcgaa
    11821 tatgacccag gaggagcgta acagcgcagg gggagccgaa aagtggagta aaaaacgcgg
    11881 cttccagtgg tctactgcca gatcctacct gaaatcgagc ggagtgacca agcaaggtgc
    11941 gagaaaactg gcgtggttaa aaaacagcgg gaatatgtcg aatccattct atctcgccca
    12001 accaaccagc cgcaggacat aagctgaagg taccgatagc ggcaattttg ttcatctgtg
    12061 ccgggtgggc aaatagcaat tgcccccgaa cactccctgc caggcaactt ataccgcatg
    12121 gcagggaaca ttcgggttaa aaagcaaaag ataactaatt actcttcagc ttagccttcc
    12181 tgcaaccgtg acggcggagc cgaatgatag tgcgcatcgg cctcggcaaa gcgtttttgc
    12241 atcgccgctg acggtgcttt acccagcaaa ctaaacacta caatcccaat actgccaaag
    12301 ataaagcccg gaataatttc gtacagtccc agccagccga actgtttcca gacgatcacc
    12361 gtcagcgcac cgatgatcat ccccgccagc gcaccgttgc gcgtcatgcg tgaccacatc
    12421 accgaaaaca gcaccaccgg accaaacgcc gcgccaaagc ctgcccacgc gtagctcact
    12481 aagcccagca cgcggttttc cgggttcgct gccaacgcaa tcgccaccag cgccaccacc
    12541 agcaccatca cacgccctac ccacaccagt tctttctggc tggcatgttt acgcagaaac
    12601 gctttgtaca aatcttcggt aatcgcactg gaacatacca gcagctggca acttaacgtt
    12661 gacattaccg ccgccagaat cgccgacagc agaatcccgg caatccacgg gttaaacaga
    12721 atttgcgcca gttcgataaa cacgcgctcg gcgttctggt ttaccgcacc agctaccgac
    12781 gggtgctcgt taaagtaagc gataccaaag aagccgacag ccaccgcccc tgccaggcag
    12841 aggatcatcc aggtcatact gatacgacgc gcatggacga tgctgtggtg agaatccgcc
    12901 gccataaaac gcgccaggat gtgcggttgc ccgaagtaac ccagccccca ccccatcagc
    12961 gagataatgg cgacaaagtt cagccctttg agcatatcca cgttttcgat gctcttttgt
    13021 ttgatcactt ccagcgagtc accaaagcca ccgacactga taatgacgat aaccggcgtc
    13081 aggatcaggg caaaaatcat caggctggcc tgtacagtgt cagtccagct caccgcgagg
    13141 aaaccgccaa taaaggtgta aaggatcgtc gccgcagctc ccgcccacag agccgtttcg
    13201 tagctcatgc caaaggtact ttcaaacaga cgcgcgcctg ccacaatgcc cgaagcgcaa
    13261 taaatggtga agaacagcaa aatcaccagc gcggagataa tgcgcaaaat gcggctttta
    13321 tcttcaaaac gcccggtgaa ataatccggt aatgttaagg cgttattgtt gtattcggta
    13381 tgcacacgca accgcccggc caccagcttc cagttaatcc acgcgcctaa tgtcaggcca
    13441 atggcgatcc aactttcgga aatcccggaa agaaaaaccg cgcccggcaa ccccattaac
    13501 agccagccgc tcatatccga tgcacccgcc gataatgccg tcacgaatgg cccaaggcta
    13561 cgtccgccca gaatatagtc gtcaaagttt ttcgttgatc gccaggcgat aaacccaatc
    13621 aatatcatgc caaagatata gacacaaaat gtcaccaaca tcggtgtgct aatagccatc
    13681 taaagtctcc aaaaaattat tatcggcaat gtcgaaactt gccgttatat ctgccaccgg
    13741 aacggggtaa cagagtttat gttttaccag ggcgaccgta tcctgccgga agcgctggtt
    13801 attcacaatc gatttaacac accatttaca ttaaatttta gtgctcagcg acactatttt
    13861 tcatcaggtt gcactctctc acattttttg cggttgcacc tttcaaaaat gttaactgcc
    13921 gcagagaaaa agtctgagtt atttttttaa tccctgtcat atcgatttct tttattaaca
    13981 tttcattcat ttttaagctt gctacgcagg tcacatttaa catggttgca caaagttgca
    14041 acatcatgga tatttcacga taacgttaag ttgcaccttt cagaacaaca ggagtaatgg
    14101 catgggaacc accaccatgg gggttaagct ggacgacgcg acgcgtgagc gtattaagtc
    14161 tgccgcgaca cgtatcgatc gcacgccaca ctggttaatt aagcaggcga ttttttctta
    14221 tctcgaacaa ctggaaaaca gcgatactct gccagagcta cctgcgctgc tttctggcgc
    14281 tgccaatgag agcgatgaag caccgactcc ggcagaggaa ccacaccagc cattcctcga
    14341 ctttgccgag caaattttgc cccagtcggt ttcccgcgct gcgatcaccg cggcctatcg
    14401 ccgcccggaa accgaagcgg tttctatgct gctggaacaa gcccgcctgc cgcaaccagt
    14461 tgctgaacag gcacacaaac tggcgtatca actggccgat aaactgcgta atcaaaaaaa
    14521 tgccagtggc cgcgcaggta tggtccaggg gttattgcag gagttttcgc tgtcatcgca
    14581 ggaaggcgtg gcgctgatgt gtctggcaga agcgttgttg cgtattcccg acaaagccac
    14641 ccgtgatgcg ttaattcgcg acaaaatcag caacggtaac tggcagtcac acattggtcg
    14701 tagcccgtca ctgtttgtta atgccgctac ctgggggctg ttgtttactg gcaaactggt
    14761 ttccacccat aacgaagcca gcctctcccg ctcgctgaac cgcattatcg gtaagagcgg
    14821 tgaaccgctg atccgcaaag gtgtggatat ggcaatgcgc ctgatgggcg agcagttcgt
    14881 cacgggcgaa accatcgcgg aagcgttagc caatgcccgc aagctggaag agaaaggttt
    14941 ccgttactct tacgatatgc tcggcgaagc tgcactgact gccgcagacg cacaggcgta
    15001 catggtttcc tatcagcagg cgattcacgc cattggtaaa gcgtctaacg gtcgtggcat
    15061 ctatgaaggt ccgggcattt caatcaagct gtcggcgctg catccgcgtt acagccgcgc
    15121 ccagtatgac cgggtaatgg aagagcttta cccgcgtctg aaatcactca ccctgctggc
    15181 gcgtcagtac gatattggta tcaacattga cgccgaagag gccgatcgtc tggagatctc
    15241 cctcgatctg ctggaaaaac tctgtttcga gccagagctg gcaggctgga acggcatcgg
    15301 ttttgttatt caggcttatc aaaaacgctg cccgctggtg attgattacc tgattgatct
    15361 cgccacccgc agccgtcgcc gtcttatgat tcgcctggtg aaaggcgcgt actgggatag
    15421 tgaaatcaag cgcgcgcaga tggacggcct tgaaggttat ccggtttata cccgcaaggt
    15481 atataccgac gtttcttatc tcgcctgtgc gaaaaagctg ctggcggtgc cgaatctaat
    15541 ctacccgcag ttcgcaacgc acaacgccca cacgctggcg gcgatttatc aactggcagg
    15601 tcagaactac tatccgggtc agtacgagtt ccagtgcctg catggtatgg gcgagccgct
    15661 gtatgagcag gtcaccggga aagttgccga cggcaaactt aaccgtccgt gtcgtattta
    15721 tgccccggtc ggcacacatg aaacgctgct ggcgtatctg gtgcgccgcc tgctggaaaa
    15781 cggtgctaac acctcgtttg ttaaccgtat tgccgatacc tctttgccac tggatgaact
    15841 ggtcgccgat ccggtcactg ctgtagaaaa actggcacaa caggaagggc aaactggatt
    15901 accgcatccg aaaattcccc tgccgcgcga tctttacggt cacgggcgcg acaactcggc
    15961 agggctggat ctcgctaacg aacaccgtct ggcatccctc tcttctgccc tgctcaatag
    16021 tgcactgcaa aaatggcagg ccttgccaat gctggaacaa ccggtagcgg caggtgagat
    16081 gtcgcccgtt attaacccgg cggaaccgaa agatattgtg ggctatgtac gtgaagccac
    16141 gccgcgtgaa gtagagcagg cgctggaaag tgcggttaat aacgcgccaa tctggtttgc
    16201 cactcctccg gctgaacgcg cggcgatttt gcatcgcgct gccgtgctga tggaaagcca
    16261 gatgcagcaa ctgattggta ttctggtgcg tgaagccgga aaaaccttca gtaacgccat
    16321 tgccgaagtg cgtgaagcgg tcgattttct ccactactac gccggacagg tgcgggatga
    16381 tttcgctaac gaaacccatc gtccattagg gcctgtggta tgtatcagcc cgtggaactt
    16441 cccgctggcg attttcaccg ggcagatcgc tgccgcactg gcggcaggta acagcgtact
    16501 ggcaaaaccg gcagaacaaa cgccactgat tgccgcgcaa gggatcgcca ttttgctgga
    16561 agcgggcgta ccgccaggcg tggtgcaatt gctgccaggt cggggtgaaa ccgtgggcgc
    16621 gcaactgacg ggtgatgatc gcgtacgcgg ggtgatgttt accggttcaa ccgaagtcgc
    16681 tacgttactg cagcgcaata tcgccagccg cctcgacgct cagggtcgcc ctattccgct
    16741 catcgctgaa accggcggca tgaacgcgat gattgtcgat tcttcagcac tgaccgaaca
    16801 agtcgtaata gatgtgctgg cctcggcgtt cgacagtgcg ggtcagcgtt gttcggcgct
    16861 gcgcgtgctg tgcctgcaag atgagattgc cgaccacacg ttgaaaatgc tgcgcggcgc
    16921 aatggccgaa tgccggatgg gtaatccggg tcgcctgacc accgatatcg gtccggtgat
    16981 tgatagcgaa gcgaaagcca atattgagcg ccatattcag accatgcgta gcaaaggccg
    17041 tccggtgttc caggcggtgc gggaaaacag cgaagatgcc cgtgaatggc aaagcggcac
    17101 ctttgtcgcc ccgacgctga tcgaactgga tgactttgcc gaattgcaaa aagaggtctt
    17161 tggtccggtg ctgcatgtgg tgcgttataa ccgtaatcag ctgccggagc tgatcgagca
    17221 gattaacgct tccggctatg gtctgacgct tggcgttcat acgcgtattg atgaaaccat
    17281 cgcccaggtc actggctcag cccatgttgg taacctgtat gttaaccgta atatggtggg
    17341 cgcagtggtt ggggtgcagc cattcggcgg cgaagggttg tccggtaccg ggccgaaagc
    17401 aggcggtccg ctctatctct accgtctgct ggcgaatcgc ccggaaagtg cgctggcggt
    17461 gacactcgcg cgtcaggatg cagagtatcc ggtcgatgcg cagttgaaag ccgcattgac
    17521 tcagccgcta aatgcactgc gggaatgggc agcaaatcgt ccagaattgc aggcgttatg
    17581 tacgcaatat ggcgagctgg cgcaggcagg aacacaacga ttgctgccag ggccgacggg
    17641 tgagcgcaac acctggacgc tgctgccgcg tgagcgcgta ttgtgtatcg cggatgatga
    17701 acaggatgcg ctgactcagc tcgccgccgt gctggcggtg ggcagccagg tgctgtggcc
    17761 agatgacgcg ctgcatcgtc agttagtgaa ggcattgcca tcgactgtca gcgagcgtat
    17821 tcaactggcg aaagcggaaa atattaccgc tcaaccgttt gatgcggtga tcttccacgg
    17881 tgattcggat cagcttcgcg cattgtgtga agcagttgcc gcgcgggatg gcgcaattgt
    17941 ttcggtgcag ggttttgccc gtggcgaaag caatatcctg ctggaacggc tgtatatcga
    18001 gcgttcgctg agtgtgaata ccgctgccgc tggcggtaac gccagcttaa tgacgatcgg
    18061 ttaaacaggt agccggagga tgttacaccc tcctccggca tcattagcgt ggtcgaatcc
    18121 cctcaataat aatccgctgc acgttttcaa ccgtttgatt gaaaaatacc tcatcgcgca
    18181 acgttgcgcc tgtcaccgcc tccacctgag gggcgaaatc ggcgtaatgt tgagtggaag
    18241 cccaaatcat aaaaatcaaa tgctgcggat caatcggcgc gagtttgccg cttttgaccc
    18301 aaccggcaat cagcgccgat ttctcatcaa ttaatgactt caaatcgccc gtcagttcat
    18361 ccattaacag cggcgcgcct gccagcatct ccatacagaa caggcgcgaa gcctgcggat
    18421 aatcgcgtga gacttccagc ttcagacgga tgtactcttt gatcgccgcc agcggggcga
    18481 aatcttcacg aaacgctttt aacggtgcca gccagatatc gagaatctgc cgcagcacgg
    18541 caatatacag cgcctctttt gacggaaagt aatacagcag attggttttt gaaacacccg
    18601 ccaactctgc aatttgctcc agccgtgtgc cgtgaaaacc gaattgtgaa aaagtgtcca
    18661 gtgctgcgct aagaatcgct tttttcttcg cgcttactgc gcgcgaacgt ttacccgttg
    18721 ttttcactgc gccttgcgtc atgcgctctc ccctcttgta gattggcaac aggatagcaa
    18781 aacccccgcc cggcctcgac cttctgcact ctcatcgcgc acagatgcat gttttatgtg
    18841 caactgtttt gaccgtttag tccacttttt accagacatt ttagataacg cgatgttaac
    18901 acattaataa cactcccttt ttaaaaactg gcatccgctt tgcaaacaag ccaatacgcg
    18961 gctgaaaagg atatgcagaa tgcagcgccc cgtctgactt tcactttacg agacgaagag
    19021 aggttgatga tgaaaattgg cgtattcgta cctattggca acaacggctg gctcatttcg
    19081 acccacgcgc cgcagtacat gccgaccttt gaactgaata aagccatcgt gcaaaaagcg
    19141 gagcactacc atttcgattt cgccctgtcg atgatcaaac tgcgtggctt tggcggcaaa
    19201 actgagttct gggatcacaa ccttgagtcg ttcaccttga tggcggggct ggcggccgtg
    19261 acctcgcgca ttcagattta cgccaccgcc gccaccttaa cgttacctcc ggcaatcgtc
    19321 gcccgtatgg ccgcaaccat cgactccatc tctggcgggc gttttggcgt caacctcgtg
    19381 actggctggc aaaagcccga gtatgagcag atgggtatct ggcctggcga tgactatttc
    19441 tcccgtcgtt acgactatct caccgagtat gttcaggtgc tgcgcgacct gtggggcacg
    19501 ggaaaaagcg attttaaagg cgattttttc accatgaatg attgtcgcgt cagtccgcaa
    19561 ccgagtgtcc ccatgaaagt gatctgcgcc gggcaaagcg acgctggcat ggcgttctcc
    19621 gctcagtatg ccgatttcaa cttctgtttc ggcaaaggcg taaatacacc cacggctttc
    19681 gccccgaccg ctgcgcggat gaaacaggcc gcagagcaaa ccgggcgcga cgttggctct
    19741 tatgtattgt ttatggtgat tgccgatgaa accgacgatg ccgctcgcgc caaatgggaa
    19801 cactacaaag cgggcgcgga tgaagaggcg ttaagctggc taaccgaaca aagtcagaaa
    19861 gatacccgct ccggtactga caccaacgtc cgtcagatgg ccgatcccac ttcggcggta
    19921 aacatcaata tggggacgtt agtcggttct tacgccagtg tcgcgcgcat gttagatgaa
    19981 gtcgcaagcg tgcctggtgc cgaaggcgtg ctgttaacct tcgacgattt tctgtcggga
    20041 atcgaaacct tcggcgagcg cattcaacca ctgatgcagt gccgcgccca tctccctgtg
    20101 ctgactcagg aggtggcatg atgacgacat taaccgctcg accggaagcc attaccttcg
    20161 atccgcagca aagtgcgctg atcgtggtgg atatgcaaaa cgcctatgcc acgccaggcg
    20221 gctacttaga tctcgccggg tttgatgtct caaccactcg cccggtcatt gccaacattc
    20281 aaaccgccgt gaccgcagcg cgagcggcag ggatgttgat catctggttt caaaatggct
    20341 gggatgaaca gtatgtcgag gctggcggcc ctggctcacc gaattttcat aaatcgaacg
    20401 ccctcaaaac catgcgtaaa cagccgcagc tgcaggggaa attgctggcg aaaggctcct
    20461 gggattatca actggtggat gaactggtgc cgcagcctgg cgatattgtg ctgccgaagc
    20521 cgcgctacag cggtttcttc aatacgccgc tggacagcat tttgcgcagc cgcggaatac
    20581 gccatctggt tttcaccagc atcgctacca acgtctgcgt cgaatcgacg ctacgcgatg
    20641 gcttttttct ggagtatttc ggcgtggtgc tggaagacgc aacacaccag gcggggccgg
    20701 aatttgtaca gaaagccgcg ttgttcaata tcgaaacctt ttttggctgg gtcagcgacg
    20761 tcgaaacgtt ctgcgacgcg ctttctccca cgtcctttgc tcgtatcgct taaggagttt
    20821 aacgatgcca aaatccgtaa ttattcccgc tggcagcagc gcaccgctgg cccccttcgt
    20881 tcccggcacg ctggctgatg gcgtggtgta tgtctccggt acgctggctt ttgatcaaca
    20941 caataacgtg ctgtttgccg atgacccaaa ggcgcaaacc cgccacgttc tggaaactat
    21001 ccgcaaggta atcgagacag cgggtggcac gatggcggat gtgaccttca acagcatctt
    21061 tattaccgac tggaaaaatt acgccgcgat taacgaaacc tacgccgagt tttttccagg
    21121 tgataaaccg gcgcgattct gcattcagtg cggactggta aaacctgacg cgctggtgga
    21181 aatcgccaca attgcgcata tcgccaagtg aggccgcgat gaaactttca ctctcacctc
    21241 ccccttatgc tgatgcgccc gtagtggtgt tgatttcggg tcttggcggt agcggcagtt
    21301 actggttacc gcaactggcg gtgctggagc aggagtatca ggtagtctgt tacgaccagc
    21361 gcggcaccgg caataatccc gacacgctgg cagaagatta cagtatcacc cagatggcag
    21421 cggaactgca tcaggcgctg gtagccgcag ggattgagca ttacgcagtg gtcggccatg
    21481 cgctcggtgc gctggtggga atgcagctgg cgctggatca tcccgcgtcg gtaactgtgc
    21541 tggtctgcgt taacggctgg ctacgaataa acgcccatac gcgccgctgt tttcaggttc
    21601 gcgaacgatt actgtatagc ggtggcgcgc aggcatgggt ggaagcgcag ccgttgttcc
    21661 tctatcccgc cgactggatg gcggcccgcg cacctcgcct tgaggcagaa gacgcgctgg
    21721 cactggcgca ttttcagggc aaaaataatt tactgcgtcg acttaacgcc ctcaaacgcg
    21781 ctgactttag tcaccatgcg gatcgcatcc gctgcccggt gcaaatcatc tgcgccagtg
    21841 atgatctgct ggtgccatca gcatgttcca gtgaacttca tgccgccctg cccgatagcc
    21901 agaaaatggt gatgcgctat ggcggacacg cctgcaacgt gaccgatccc gaaacgttta
    21961 atgctctgtt actcaacggg cttgccagcc tgttacatca ccgtgaagcc gccctgtaag
    22021 gaattgctat gaacgaagcc gttagcccag gtgcgcttag caccctgttc accgatgccc
    22081 gcactcacaa cggctggcgg gagacacccg tcagcgatga gacgttacgg gagctttatg
    22141 ccctgatgaa atgggggccg acatcagcta actgttctcc ggcacgtatc gtgtttatcc
    22201 gcactgcaga gggaaaagaa cgtctgcgcc cggcgctttc cagcggcaat ctgcaaaaaa
    22261 ccctgaccgc gcccgtcacc gctatcgtcg catgggacag tgaattttat gaacggttac
    22321 cgcaactgtt tcctcacggc gatgcccgca gttggtttac ctccagccca caacttgccg
    22381 aagaaacagc gtttcgcaac agttccatgc aggcggctta tctgatcatc gcctgccgtg
    22441 cgctggggct ggataccggc ccgatgtcgg gttttgatcg tcaacacgta gacgacgctt
    22501 tttttgcagg cagcacgctg aagagcaatc tgctgattaa tatcggctat ggcgatagca
    22561 gcaaactttt tgcgcgcctg ccacgtctgt cctttgaaga agcctgcggg ctgttgtaag
    22621 gagcaaacta tgaacattgt cgatcaacaa acttttcgcg atgcgatgtc ctgcatgggc
    22681 gcggcggtca atatcatcac cacggacggt ccagccgggc gcgccgggtt caccgccagc
    22741 gccgtctgca gcgtgaccga cacgcctccc acattactgg tgtgcctgaa tcgtggggcg
    22801 tccgtctggc cggtattcaa tgaaaatcga acgctgtgtg taaatacact tagcgccggg
    22861 caggagccgc tttcaaacct ttttggcggc aaaacgccca tggaacaccg ctttgccgcc
    22921 gcccgctggc agactggtgt gaccggatgc ccgcaactgg aagaggcgct ggtttcgttt
    22981 gactgccgta tcagccaggt ggtgagcgtc ggcacccacg acattttgtt ttgcgccatc
    23041 gaagcgattc atcgtcacgc caccccctac gggctggtgt ggttcgatcg cagttatcac
    23101 gcgctgatgc gccctgcttg ttaacctcct taaggagaca gctgatggca atgttcggtc
    23161 ttcctcactg gcagttaaaa tcgacctcta cagagagcgg cgtggtcgcg ccggatgaac
    23221 gattgccgtt tgctcagacg gcagtaatgg gcgttcaaca cgcggtggcg atgtttggcg
    23281 cgacggtgtt aatgccgatt ctgatggggc tggatcccaa tctttccatt ttaatgtcag
    23341 ggatcggcac gttactgttt ttctttatta ccggcgggcg cgtgcccagt tatctcggct
    23401 ccagcgcggc ttttgtcggc gtggtgatcg ccgcaacggg ctttaacggt cagggcatta
    23461 acccgaatat cagcattgcg ctgggcggga ttatcgcctg tgggctggtt tataccgtca
    23521 ttggcctggt ggtgatgaaa atcggtacgc gctggattga acggctaatg ccgccggtgg
    23581 tgacgggtgc ggtggtgatg gcgattggcc tgaacttagc gcctatcgcg gtaaaaagtg
    23641 tatcggcttc aggctttgat agctggatgg cggtaatgac ggtgctgtgt attgggctgg
    23701 tggcggtgtt tacccgtggc atgatccagc ggttattgat tcttgtagga ttaattgtcg
    23761 cctgtctgct gtatggcgtg atgaccaacg ttttagggct gggaaaagcc gtcgatttta
    23821 ctctcgtcag ccatgctgcc tggttcggtc tgccacattt ttccactcct gcttttaatg
    23881 gtcaggcaat gatgctgatt gcgcccgtgg cggtgattct ggtggcggaa aacttaggtc
    23941 atctcaaggc ggtcgccggg atgaccggac gcaatatgga cccgtatatg gggcgagcat
    24001 tcgtaggcga tggtctggca acgatgcttt ctggttctgt cggcagtagc ggcgtcacca
    24061 cctatgctga aaatatcggt gtgatggccg tgacaaaagt ctactcgacg ctggtgtttg
    24121 ttgctgctgc cgtcatcgcc atgctgttgg gcttttcacc gaaatttggc gcgctgattc
    24181 ataccattcc ggccgcggtg attggtggtg catcaattgt tgtgttcggg ctgattgccg
    24241 tcgcaggcgc aaggatatgg gtacaaaacc gtgtcgattt aagccagaac ggtaatttga
    24301 ttatggtcgc agtaacctta gtgctgggcg cgggtgattt tgctctcacg ctgggcggtt
    24361 ttacgttggg aggaatcggt acagcaacct ttggcgcgat tttactcaat gcgttattaa
    24421 gcagaaggtt ggttgacgtt ccaccgccag aagttgttca tcaggagcca taaacagaaa
    24481 accgccgggg ttaccggcgg tgatggcttt ttgacgagct taagaaaaaa atgcattcgt
    24541 cattgattag cccggctagt tgtcgctttt gccgtgactg ctcttgccac cttttttacc
    24601 tgcttctgat gctctttcgc ggtcttctgc aaaattgccg gaaccgcctc gatggtttgc
    24661 cattactgac ctccgtctgg gtatagacat catattgcta gaattaacgc ggcgtatggc
    24721 ggctgccata ttttcgctac gtgggactaa gcttagtgaa ctgacgtgtt tctacccgcg
    24781 tttggtaata tcctgcaaca gcttcttagc gagaatagat atcatctcca ataattatcc
    24841 ataagccgct cttatgatta aaaaaacgcg atgagataaa aatgtgtcat tttgcgacaa
    24901 aattgcgtgc ttgtgaaagt tgttataaat caaataagtg gttgtgaaat ttgcactctg
    24961 aaaaggacgt cttatcttta aataagtggt agcgaatcgc tacggaatag agataacacg
    25021 aggagtggtt agaaatggct aaagttctgg tgctttatta ttccatgtac ggacatattg
    25081 aaacgatggc acgcgcagtc gctgagggtg caagcaaagt ggatggcgct gaagttgtcg
    25141 ttaagcgtgt accggaaacc atgtcgccgc aattatttga aaaagcaggc ggtaaaacgc
    25201 aaactgcacc ggttgcaacc ccgcaagaac tggccaatta cgacgccatt atttttggta
    25261 cacctacccg ctttggcaac atgtccggtc aaatgcgtac cttcctcgac cagacgggcg
    25321 gcctgtgggc ttccggcgca ctatacggaa aactggcgag cgtctttagt tccaccggta
    25381 ctggcggcgg tcaggaacaa accatcacat ccacctggac gacccttgcg catcacggca
    25441 tggtgattgt ccccattggc tacgcagcgc aggaattatt tgacgtttca caggttcgcg
    25501 gcggtacgcc gtacggcgca accaccatcg caggcggtga cggttcacgc cagcccagcc
    25561 aggaagaact gtctattgct cgttatcaag gggaatatgt cgcaggtctg gcagttaaac
    25621 ttaatggcta atcttcaaca ggaggatacg catgccaact caagaagcga aagctcatca
    25681 cgtcggtgaa tgggcatctc tgcgcaatac gtcgccggaa atagccgagg ccattttcga
    25741 agtcgccggg tacgacgaaa aaatggcgga aaagatttgg gaagaaggta gcgatgaagt
    25801 gttagtcaaa gcctttgcta agaccgacaa agattcgctt ttttggggcg aacagaccat
    25861 cgaacgtaaa aacgtttaac cagcgactcc cccgtagcga acgggggagt tttctgttat
    25921 ttcaccgctt cattcaacac gctatcaaac ttatccatcg ggcagaaacc atcagcgtct
    25981 atcgggcaac cgcttaattc cagcgtcacg cgctgcgcag gtgcctgcag ggttaacgca
    26041 tcggcattac gtaactgttc cgcactctgg tacacatatt caattttcat caaatcgcga
    26101 ttggctttgc tgtcacgcca acgctggaaa acgattttgc cgccaatcgg cgtgcgttcg
    26161 ttctggtcat gcaactgata cggtttgaaa tccagcgccg ttaacagaga ggcaatgttg
    26221 gagtcatgcc caactaacac cgtgattttt ggagcactgg tgcgatcggt gaccagggct
    26281 ttgtcgatat aactgaccag cggtttcgca acattgcgcg ccacttccgg tgaggtaaac
    26341 aggctgtcct ggtaaccgtt tttcagcttc gacaacacct tccactgctg gtcagatttg
    26401 atttctcccc aggccacctg atccatcgga aaaccttcgt agtattgcaa agtaaacgca
    26461 tctaccagcg agttgccgac tttcagcggc ccggaaacac ctggttcttg ttgatacttg
    26521 gcgctaaagg tatttttgcc atccaccagc gaacactgtt gtttctcttt acaggcaggg
    26581 gaatctttat agttaacgat tttttccagt agctggtagc tgtcggtaag ctggagtttg
    26641 ctgagctctt tctccattgc cgccaccgcc tgttcactga atgcggcgga atcatcggtg
    26701 atcaccgggt taaaggttgg gtccatagtg cccatttttt cctggtgatg cacaggaata
    26761 tcacaccccg ggaatgcgcc ggtaataaag aactgtgcgg tcgcaacggt acgttgcaga
    26821 ctattggcat aggcataaac ggtgtccggc ggcgggcatt cccccgattt taccatcccc
    26881 tgctctgcca gccattcacg catgtaatgg cccatataca cttcgagtac gccacctttg
    26941 gtggtgagtt gcccaccggg gacgtcccat tcgggccatt tattcggcgt cgactgctcc
    27001 agcacactgc cattgttcgc cagcggcgca cgtaagttat ggcggctcat catgagcact
    27061 tgctgtagct gatagccttc cggtacggtt tgtgcctgag cgtttgaagc gagtaaaact
    27121 acccctgcca cagctgcggc gattagcgtt ttgttcattc ctggcacctc ttttgttatc
    27181 aatcactaaa gagtgtgaca gaaatatgac ttttttccgg gaacaggttc gcaaaatggc
    27241 ttgttgaacg gctgttttta tattcatctt taaaaacagg caaggtttga cgcaaactat
    27301 gcctgattag cccctgcctc gcgtacgccg ctgatgattg cacctgtggc taaacagaat
    27361 atcggcggcg ttaccaatac agaggcaata tttaccaata aaaaagtgtg agactaataa
    27421 caatggtttt tagcatatgc ctctgtcatt aaattgcata acgagttaaa aaaccaattt
    27481 gcgcattcct ttccttattg tttaactcgt aagcctgagc aaaaagttca ggagggaggt
    27541 aaacgtcata atgattgcca gtgtttatta aataaccata ctcaagatct tcatctgttg
    27601 aaaaggattc attccctaag gagattgatg ccacattact attaacaata aataatgagc
    27661 aaatgctatc tgaaagagca gtgaggaaat ttttcacctc attaatcctc ttctctgggt
    27721 cggtgtctaa aacaaggcgc gccttgttat cattaaatat aaactcgggt aaatgcagta
    27781 tgcatttttt gtcattcaag caactatcaa taaattcttt ttttaaaaaa cagaaagttc
    27841 catgacataa gactaccctg cccctgaatt ttgttgtcag attgtagatc aacgccaaat
    27901 aacgtcttta catcttccct gagattatag aatgccgtat caacaagagc ctcgatcgtt
    27961 tgtttatcaa caattttaat aaactcttcg tatacttgct tttgcgtctt atggctgccc
    28021 agcgcccatg aatactcatg ttcttttaaa accagtatgg cccttaacag acaatagcca
    28081 tcgccatcta ctggagcgta ttttaatcca taagttttat taatttcact ctgaataaaa
    28141 gatataccag acaaatcaat accagaatct aaatcagata ttcccggcgt atttatccat
    28201 tctggctgga tactcatatt ttcatcatta tcaaaacaat aatcagaata aacatcatca
    28261 cacgcattaa tgttttcaat atattccata aagccatacc aataataacc tttaaaatct
    28321 gtgatagact ctgaacctct acccggcaag gatactctaa caatatcaac atcttcaaca
    28381 tattgtatag tatatgtaat acctcctgaa acccctgaat aaatttgttt atcattagta
    28441 cgcaatgttt tcataaataa tgataaatca ttatcattct gtatttcctc aaattctttt
    28501 tctagtgaat cgcattccag gcatggaatg tagtcttcag cttgttgacg tctgatttct
    28561 ttatacaacc gcataaaaca caccagtgtg ttttcaaaaa gcgtgattga atatgtttta
    28621 tttttttcgt actcattttt tatatcgttc caggcttgct tcaaataatt atttgcagtg
    28681 caactaattc catgtatatt acccatatat agcgcctata aaatttaata aataataacg
    28741 cactagtaaa acttaaagtg cctgtttcaa ctatcaaaaa tcgctcaccc tttttcacct
    28801 gtttaaaata tgttcagcgc cccaccttga ggcgacctcc tctccgcgat gatttcaata
    28861 acatattctg tgttggcata tgaaattttg aggattaccc tacacttata ggagttacct
    28921 tacaggggtt ccttcaattt gtgttgattt acgcgagata acgctatgga attaaaggat
    28981 tattacgcca tcatgggcgt gaaaccgacg gacgatctca agacaatcaa gaccgcctat
    29041 cgtcgacttg cccgcaaata ccatcctgat gtcagcaaag aaccggatgc cgaagcccgc
    29101 ttcaaagagg tcgctgaagc ctgggaagtg ttaagtgatg aacaacgtcg cgctgagtat
    29161 gatcagatgt ggcaacatcg caacgatccg caatttagcc gtcagttcca gcatggcgac
    29221 ggtcagagtt ttaacgccga agattttgac gatatcttct cgtcaatttt cggtcagcat
    29281 gcccgccaga gccgtcaacg ccccgccgca cgcggccacg atattgaaat cgaagtggcg
    29341 gtattcctcg aagaaacgct tactgagcat aagcgtacca tcagctataa cctgccggtt
    29401 tataacgcct ttggcatgat cgaacaggaa atcccgaaaa cgctgaatgt gaagatcccg
    29461 gcaggcgtcg gcaatggtca acgcatccgc ctgaaaggcc aggggacgcc gggcgaaaac
    29521 ggcggtccaa atggcgattt gtggctggtg attcatattg cgccacatcc gctgtttgat
    29581 attgtcggcc aggatctgga aattgtggtg ccggttagcc cgtgggaagc ggcgctgggt
    29641 gctaaagtca ccgttccaac actgaaagaa agcattttgc tgactatccc gcctggcagc
    29701 caggccgggc aacgattgcg cgttaaaggc aaaggtctgg tgagcaaaaa acagaccggc
    29761 gatctgtatg cggtactgaa aatcgtgatg ccgccgaaac cggatgaaaa cactgccgcg
    29821 ctgtggcagc aactggcaga cgcccagtcg tcttttgatc cacgtaaaga ttgggggaaa
    29881 gcataatggc taatgttacg gtgactttta ctattaccga attttgcctg cataccggca
    29941 tctctgaaga ggaattgaat gaaattgtcg gtttgggggt ggttgaaccg cgtgagatcc
    30001 aggaaacaac ctgggtattt gacgaccatg ccgccatcgt ggtgcaacgc gcggtgcgcc
    30061 tgcgtcatga actggctctg gactggccgg ggatcgcggt ggcgctgacg ttaatggatg
    30121 atattgcgca cctgaagcag gaaaaccgcc tgctgcgcca gcggctttcc cggtttgtag
    30181 ctcatccgtg atgtgtgttt gccggatgtg atgacgcatc ttagcctgcc tgcaaagagt
    30241 acttttcctg tgggccggat aaggcgtgtt cacgccgcat ccggcggcgg tgccctaatg
    30301 cctgatgcga cgctaaacgc gtcttatcag gctaacaaac gacgttatct gttttggtgg
    30361 tcgccctcaa ctaacaccag caataactgg cttagtgccg cgtaaaaacc aaagctgtca
    30421 tactgatggc aacgcgcaac aaactctggt aaccattgcc gcaacgccgt gagtgttttt
    30481 tgccgtaaac tgtcgattct tcgcgcagga accgtcccct ctcccagcga aaaatgcaga
    30541 tgactcaaca actcgagata gatcgccaga tgatctgtcg gttcgttgaa attgccgctg
    30601 gtttccatcc ctgcctcaac taacaagcgt ttaatctctt gctcgtcctg tttgtaggcc
    30661 gatgcatacg gcagcgccgc ttgtttgtca gtcatcagaa acaggccgca aaagtcagcg
    30721 gccagttcca gacgggcatc gtcacgtact gtcagagtgg caacacggtt ttccagctca
    30781 tccaccgccg cagtgagcgg cggttcgctt ttcagcaacg aaaaccattc agccatctgc
    30841 gcactggcga tttgcgtcag ttgttcatcg tccagttcac gggagaacaa ctgcgccagc
    30901 caggcgtaaa cacaggctat ctgttgtgct gtcagcgtgg tcataatttc acctgcgacg
    30961 cgggaacata ttcgcactgc gccaccatct ccacggggcc gttaaacgcc gttacctgct
    31021 ccactgttcc gttgcacttc tcaatttcca ccagcgtagt gtgcgcactg gtcgcctgcg
    31081 ccagctgcga agtaccgatg tcaatggtca acacgttggg gttaccgtat ttgcacagcg
    31141 caccaggctc gccgccttta tctggatcgt gccatgcccc ttcgtgaatt cgcgccacgc
    31201 cgggtgcata gcggtcagaa actactgccc ctgccaacac ctgaccgcga gcgttaaaga
    31261 cgcgtaccac atcaccgtta cgaataccgc gcgcgctggc atcctgcggg ttaatgaaca
    31321 ctggctcttt acccgctacc gtatattgct gacgcagagt ttccgactca cataactgcg
    31381 agtgaagtcg gaaatccgga tgcacagatt gcagatgcaa cggatacgtc tgcgagccgg
    31441 gcccaccgtg ggagcgttcg attttctcaa accacatcgg atgcccctga caatcgtcgt
    31501 agttcatatc ggcgatggtt ttcgaataga tctcaatcag gccactcggc gtgcccagcg
    31561 gttcgagatc tggatcttcg cggaatgcct ggtggcgaac aaacatctgc ggatggtcaa
    31621 actcgacgta ctctttgtta ttccagaagt catcaaacgc tggcaaatga acgccgcgtc
    31681 ctttgccttg ctgaacacct tcctgccaga tgcgtttcag ccagcccatt tcgtccagcc
    31741 cttcggtaaa ggcttcttcg cgattaaagc ggcggcacag ctcgcggaaa atatcaaagt
    31801 cgttgcgcgc ctcgaactgc ggcggcacga cctgtttcat ggcgataatg ccacggttgg
    31861 agtggttgcc gtactggtcg agatcgttac gctcaaactg cgtggtcgca ggcagcacga
    31921 tatcggcaaa gcggcaggtt gaggtccact ggttatctat ggcgataacc gtttccagtt
    31981 tgcgccagcc ttcaataatg cggttgatct gctgatggcg atggaagggg ttagttccgg
    32041 caaaaataca cattttcagc ggcggcagtt ttaccgattt accgttccag ttgattactt
    32101 tccccggttc gaggatcgca tcgataaaac gggcaatcgg aatggtgctg ctgtagcctt
    32161 tgtaatcact gttgtcgtga acaggcggaa tcgacgtaga gccggagaaa ccactcagaa
    32221 taacgccttt acgccccggc gtgcctgcgc cgttatagtg ccagccaaaa ccaaagccac
    32281 cacctggcag gccaatttgc cccagcatcg ccgccagaac cacaatcatc cacgcccact
    32341 gttcaccgtg ctgcatacgc tgtacgcacc agccagcaat aatctgcgtt ctgttcgccg
    32401 ccatctgccg cgccagtcca cgaatggttt cggcatcaat gccggtcagt ttttcagccc
    32461 atgcggcatc tttcggctga ccgtctttct cacccagcag atacggcagg aactgctcaa
    32521 aacccacaca gtagttagcg aggaagtttt tgtcgtacag gttttcactg tacaacgtat
    32581 gcgccagcgc cagttgcagc ggcacgtcag tttgcgggtt aaccgcaatg tgcttcacat
    32641 gctcgcgccc cagatactca tgggtggatg taacaaccgg atcgatgcta atgacctcaa
    32701 tttcaccggc ggcgactttc gctttcaact gcgcgtaata ttcataaaca tcgtgatccg
    32761 ggcaccacca gttcgcttgc tggtttttca gcaaatcgga gccccacagc acaatggttt
    32821 tgctgttctg caataccagc ggccaggagg tttgctgttc atacacttcc atcgaaccga
    32881 ctacgcgcgg caggatcacc tgcgcagcac cagtagagta atctccgccc gtaccaacgc
    32941 tattaccatg caaggcaata gctttcgcca gcatccccga agcgttatgg aacatccccg
    33001 tcgattgcca accactggcg gtcagcaagg cactcggccc gtgagttttc tgtacgcgtt
    33061 ccagttcttc atagaacatg tcgagggctt catcccagct cacacgcaca aaacggttat
    33121 caccgcgctg ggaggtgtcg ctcagatggc gcttacgcag ccagtccacg cgtaccatcg
    33181 gataacgaat acgcgccgcg ttgtgtacat gatccggcaa tccggcaatc attttcgacg
    33241 gatatttatc cagttcgaac ggttttgccg ccacaaagcg accatccttc accgtcgcgc
    33301 ggatagcccc ccagtgcgac ccggtaagaa tgccctcttt cgagatgaca gcctcagtcg
    33361 ccgcttgcgc cgcagtcgca cggcgcgacg ttaacaatga cggccccagc atcccggcga
    33421 cggttaagcc gccgagttgt gccagaaaac gccgacgtga tgcctgaaag agatcgttat
    33481 tgttcattat ttttcttcct tcttatcgcc gtgagcctta cctgcggtgt cagacgcatt
    33541 catttgcaga tatttcaaca aggtgcgttc ttcacgttta tcgaggctgg taaagccgat
    33601 catgccgttg agcgtgccaa tccagccgtt agcgtcaaag tgagagattt ccggtgcgcc
    33661 gtggcactgg ttacaggtgc cgttgtacaa cgaatccgca taagcccaga tcggtttgat
    33721 atcgttcacc atgtcacctt tcttcatcca cgcggtggcc tgcaacttgc tccactcggt
    33781 attggtgtcg gcaacggtgg ttttctccag cgtttttacc tgctgctgca catcaccacg
    33841 aatcgaggca acaaagatgc gtttacctgg gaattgggtg agtacacgct gacgtccggc
    33901 gctttctgtc cagccggtaa tttcaatttg cagccagtcg ccgtcacgtt taaggacttt
    33961 cacttccgag gcaggcagca gagaaccaga ggcttcttta tcgcctttcg ccgcataaat
    34021 tggcttaata tcaatagagt acagcgtgtc accactgtca ttagcaccgg cgcgcagctc
    34081 atcgaactgc ttacggaagc cgctactcat atccggtaac tggtgggcaa tacctttatg
    34141 acagtcgatg caggattgat tatctttcgc tgccaccttc atctgacgtg ccgcttcagg
    34201 atgctgcttc gcatgatcca tcgcatcgta gttatggcag gagcgacagg ttgccgagtt
    34261 gttttctttc attcgcgccc attcacgctc ggcaagttcc gcgcgtttgg cttcaaattt
    34321 ttcaggtgta tcaatggagt gggcaataaa ggtctggtag atatcgttgc tcgcttccag
    34381 tttgcgcttc accatgcctg gaatatccgg cgggatatga cagtcatggc attcagctcg
    34441 cacgccggag gcgttctgga aatgcaccga ctgtttatat tcttcataca ctggttgcat
    34501 actgtggcaa ctgacacaaa attcggttgt gctggtgact ttgatcccaa cgtgtggcaa
    34561 tacaatcagc gcaatgccaa tcacaatccc aatagcgacc agcgccagta ccgaccaacg
    34621 agcactgggt cggcgtagcg cgttccagag tttccgcata atagcccctg taaaattatg
    34681 gtttagtgaa gcgatcttaa tgagtaaata tgaacagcgg cactggtcag gatgaacggc
    34741 ttacggcaga atatgaacag atatgaacag aatgagtaaa accctctgat gccacatcac
    34801 attgttattg ttgaagatga gccggttacc caggcgcgat tacaatccta cttcactcag
    34861 gaggggtata ccgtttccgt tacggcgagc ggtgccgggc tacgggaaat tatgcagaat
    34921 cagccggtgg atttaattct gctggatatc aacttgcccg atgaaaatgg cctgatgtta
    34981 acccgcgccc tgcgagaacg ctcaacggtg gggattattc tggttaccgg acgcagcgat
    35041 cggattgacc gtattgttgg gctggaaatg ggcgcagacg attacgtcac caaaccgctg
    35101 gaactgcgcg aactggtagt acgggtaaaa aatctgctct ggcgaatcga cctcgcgcga
    35161 caagctcaac cgtacactca ggacaactgc tatcgctttg cgggttattg cctgaatgtg
    35221 tcgcgccata cgctggagcg ggatggtgag ccgattaaac tgacccgcgc agagtatgaa
    35281 atgttggtgg catttgtgac caatccgggc gaaattctca gccgtgaacg tctgctgcgt
    35341 atgctctctg cgcgtcgggt ggaaaaccct gacctgcgca ccgtcgatgt gttaattcgt
    35401 cgtttacgcc ataaactcag cgcggattta ctggtgacgc aacatggcga aggttatttc
    35461 ttagccgctg atgtgtgctg ataaaaatag accggacgaa atcccccagg cgacagcgag
    35521 cggcggatat gttcgcggtc ggcatttttc ggcgtcagaa ctaaaatcgg tgggctgaca
    35581 ttatcagaca ccgattgccc ctgtaattgc ctgatggcct gctcaactgc cagttccccc
    35641 tgccagacca tttgatcgct ggcagccata atcactcttc cccgcttcag cccgcgatac
    35701 acctgatgtg aaagataaaa cgacaccacg gtaagcggcg ttttcaggtt acgcccttcc
    35761 cccattgccg cctctgccgc aatggccgtt ccggcaacga cgtcgatttc tggatggcgt
    35821 tccagcatct cctgcaacag gttacgctgg atttcaatat cgttatcacc aagcgcaata
    35881 tcaacaatac gcaccgggct tccggcaatg gctgcgcgaa aaccctcgac catctcctta
    35941 ctgcccccgg cgttatcggg tccgggcatc aacagcacat tcagtggttt accgtgcgcc
    36001 cattgcacca gatatcgccc aggttgatag cccatctgaa accagggcac accaacgcgg
    36061 cttttcacct ggggagcatc aatagcattt accagctcga tcaccggcag atttgctact
    36121 tgcttttgca gatcgggaaa tgacgtcgtg ctactaccga gcaaaatggc ctctgcgccc
    36181 cactgtttac actggtcgat ttgtgcttgc tgggtagcca actggctgta gccgcctgcc
    36241 tccagcactt ttaaatccac accgtagcgg cgagcagcct cctgcatacc atagttcaac
    36301 gataaccaat atgaatcttt caggctggga taaagcgcgc acagtttcca tacgcgtttg
    36361 gcttttagcg gcatagaggc ttgcaccgtg aaatgctgcg catcatgcca gcgcaacagg
    36421 ttatcagccg aaaatgccgg caacatgaaa agggaaagaa gtaaaaatag cagcacgcgc
    36481 atgatagcct catcaataat aaggctttat gctagatgca ttccgctttg cgactcaaca
    36541 tttttcacct taagtgcacc gaccgtgaat ttaaccctga cccgaagact ctggatgggc
    36601 tttgccctga tggcgctgtt aaccctgacc agtaccctgg tgggatggta caacctgcgc
    36661 tttatcagcc aggtggaaaa agacaatact caggcattga ttcctaccat gaatatggcg
    36721 cgccagttga gcgaagccag cgcctgggaa cttttcgccg cgcagaacct gaccagtgcc
    36781 gataacgaaa agatgtggca ggcgcagggg cgaatgctca ccgcacaaag cctgaagatt
    36841 aatgcgttgc tgcaagcgtt acgggaacaa ggttttgata ccaccgctat tgaacaacag
    36901 gagcaggaga tctcccgttc gttacgtcag caaggggaac tggtggggcg gcgtctgcaa
    36961 ctgcgccagc aacaacagcg actcagtcag cagatagtcg ctgccgccga tgagatcgca
    37021 cgcctggcgc aaggtcaggc gaataatgcg gcaacctccg ccggagcgac ccaggccggg
    37081 atttacgatt tgatcgaaca agatcagcgt caggctgctg aaagtgcact cgatcggctg
    37141 attgatatcg atcttgagta tgttaaccag atgaatgaac tgcgccttag cgctctgcgg
    37201 gtgcagcaaa tggtgatgaa tctggggctg gagcagatcc agaaaaatgc gccaacgctg
    37261 gaaaagcagc tcaataatgc ggtgaaaatt ctgcaacgtc ggcaaatacg cattgaagat
    37321 ccgggagttc gtgctcaggt cgcaacaacg ttaactaccg ttagccaata tagcgatttg
    37381 ctggcgctgt tccagcagga cagtgaaatc agcaatcacc tacaaactct cgcacaaaat
    37441 aacatcgccc agttcgcgca gtttagcagc gaagtcagtc agctggtcga caccattgag
    37501 ctgcgtaatc agcacggact ggcgcatctg gaaaaagcca gtgcacgcgg gcaatacagc
    37561 ctgttattgc tggggatggt ttcactttgc gcactgattc tgatcctctg gcgcgtggtt
    37621 tatcgctcag tcacgcgtcc acttgccgaa caaacgcagg cgctgcaacg gctgctggac
    37681 ggtgatattg actccccttt cccggaaacc gctggcgtac gggagctgga caccatcggg
    37741 cggctgatgg atgcgtttcg cagcagtgtt catgcactga atcgccaccg cgaacagctg
    37801 gcggcgcagg tcaaagcgcg tacagctgaa ttgcaggaac tggtgataga acaccgacaa
    37861 gcacgggcgg aagcagaaaa agccagccag gcaaaatcgg cgtttctggc ggcgatgagc
    37921 catgagatcc gcacaccgct gtacggtatt ctcggcaccg cacagctact ggcagataac
    37981 cccgcactta acgcccagcg tgatgatttg cgggcaatta ccgactccgg cgaatcgttg
    38041 ctgaccatcc tcaacgatat tctcgattat tccgctatcg aagcaggtgg caagaatgtt
    38101 tcggtcagcg atgaaccctt tgaaccgcga ccgctgctgg aaagtaccct gcaattaatg
    38161 agtggacggg tgaaaggtcg cccgattcgc ctggcaacgg caattgccga cgatgtaccg
    38221 actgcgttaa tgggcgatcc gcggcgtatt cgtcaggtca ttaccaacct gttgagcaac
    38281 gccctgcgtt ttactgacga agggcagatc gttttacgta gccgtactga tggtgagcaa
    38341 tggctggtcg aagtggaaga cagcggctgc ggtattgatc ccgcgaaact ggcagaaatc
    38401 ttccagccat ttattcaggt aagtggcaaa cgcggcggca ccgggctggg gctgactatc
    38461 agtagccgtc tggcccaggc gatgggcggc gaactgagcg ccaccagcac gccggaggtt
    38521 ggaagctgtt tttgtttacg cttgccgtta cgtgttgcca cggcacccgt gccaaaaaca
    38581 gtcaatcagg cggtgcgtct tgacggttta cgtttgctgt taattgaaga taacccgcta
    38641 acccagcgaa ttaccgttga gatgctgaac accagtggtg cgcaggttgt tgctattggc
    38701 aatgccgcgc aggctttaga gacactgcaa aatagcgaac cgtttgctgc cgcactggtg
    38761 gattttgatc tgccggatgt caacggcatt acgcttgccc gacaactggc acgacaatat
    38821 ccgtcgctgg ttttgattgg ctttagcgcc catgtcattg acgaaacgct gcgccagcgt
    38881 accagttcac tatttcgcgg gattatccct aaaccggtgc cgcgtgaagt gctcggtcag
    38941 ttactggcgc actatctcca actgcaagcc aataacgatc taccactgga tgtatcgcaa
    39001 ctcaatgaag acgctcagtt aatggggacg gagaagatcc acgaatggct ggcattattt
    39061 acacaacatg ccctgccgct tctcgatgaa atcgacattg cccgcgccac tcaggatagc
    39121 gagaaaataa agcgtgccgc gcatcagcta aaaagcagtt gctcgagtct gggaatgcgt
    39181 agcgccagcc agctctgcgc acaactggag cagcagccat tatctgcccc ccttccacac
    39241 gaagaaatta cacgcagtgt tgccgctctg gaagcatggt tgaataagaa agacctgaac
    39301 gcgatttgaa tcaatatcag ccagttaaga ataactagaa tagtggcatc tggtatgcaa
    39361 cttaacggac tggcaaaatc atggcagaga aaaaaagaac ccgctggcag cggcggccag
    39421 gcacgacggg cggcaaatta ccgtggaatg actggcacaa tgccacgacc tggcgtaaag
    39481 cgacgcaatt attgctgttg gcaatgaata tttatattgc catcacgttc tggtattggg
    39541 tgcgctatta cgaaacggca ggtagtacga tatttgtcgc cagaccggga ggtatcgaag
    39601 gctggctacc gattgccggt ctgatgaatc tgaaatatag ccttgcaaca ggtcagttac
    39661 cgtccgtcca cgccgccgcg atgctgttat tggtcgcttt tatcgtcatc agtctattac
    39721 tcaaaaaggc cttttgctca tggttatgcc cggttggtac gctttctgaa ttaatcggcg
    39781 ctctcggtaa caaactgttt ggtcggcaat gtgtccttcc ccgctggctg gatattcctc
    39841 tgcgcggtgt aaagtatttg ctgttgagtt tttttctcta tatcgcgtta ttgatgcctg
    39901 ctcaggcgat tcactatttt atgttgtcgc cctacagcgt ggtgatggac gttaaaatgc
    39961 tcgatttctt tcgtcatatg gggaccgcga cattaatcag cgtgaccgtt ttgctgattg
    40021 ccagcctgtt tattcgccat gcctggtgtc gttatctttg cccatatggc gcgctgatgg
    40081 gcgtggtttc gctattatcg ccgtttaaga ttcgtcgcaa tgccgaaagt tgtatcgatt
    40141 gtggcaaatg cgcaaaaaat tgcccatcac ggatcccggt cgataaatta attcaggtac
    40201 gaacagtgga atgtaccggc tgtatgactt gcgtagagtc atgtccggta gcctcaacat
    40261 tgaccttttc actgcaaaaa cctgcggcaa ataaaaaagc ctttgcgttg tctggctggt
    40321 taatgacgct actggttctg gggattatgt ttacggtgat tggttacgca atgtatgcag
    40381 gagtatggca aagcccggtg ccggaggaat tgtaccgacg cttaattccg caagcgccaa
    40441 tgattggtca ctaaattagc aacaagcgga acatcacctc gcacggggtg atgttcacaa
    40501 aatcacatta ttttgatttt gacatcataa ctttcaagcc cggtcatttt ttctcgcgcg
    40561 gtgaagcgaa tttcagagac ttctttccct gtattcttgt tcagctcggc gattttttgc
    40621 gcgataaagt cggcgatttc cgtttcggct tgttttttaa ctcttcaata ttcactgtta
    40681 acctcttctg gtcttaaaag acagcaacac gccagtaagg tgttggttcc cggtaccctg
    40741 gtcaggtaag aaatgttata agccatctcc ccgaaagatt caataatcaa caggttatcg
    40801 cttactatac atacagcgtt gaaaaaaaat aagacagata taaaaaaggc tccacaaggg
    40861 agcctgatat tattttgtga aatccagcgg tgcatggtca gtgcgtattt gcagcgcgac
    40921 gttatcaaag gtgatcatcg tcgatttaca aaaaatacac ttagcgccca aagggtttcg
    40981 ctccgtcaca tcgaatgccg atgtgcggta ctgtgaacca tgacaacatg gacagcgaaa
    41041 atggagatgt tgagtaataa cggcaacctt agagcgtaac tacgttcgct gccgcggggc
    41101 cacgttgacc ctgctcaata gaaaattcaa ctttctgatt ttcgttcagc gtgcggaatt
    41161 cattgctctg gatggcggtg aaatggacga aaacgtcttt gctgccatca tcaggagtga
    41221 taaagccaaa acctttatct gcgttaaacc attttactaa accagtcatt ttattagaca
    41281 tattttactt ccttcatttt gggcctttcg gcaaacaggg ctttaataca gaaactactt
    41341 agtgttcagg agagagactc aaaagaaggg gataatgcct gataatgaga actgctttag
    41401 taaactactt tgtatgctgt ctgtctttcc aaccgacgca gctattaacg catgataatt
    41461 gagattaagc aaattttatt ttagccgtcc ggcggtctta aatatcgctg gctttatttt
    41521 attaaccccg tatagtgcag cgcagtattt tcagtaagga atttgtttgt cccgtaaaat
    41581 gacaggaatt gtcaaaacct ttgatcgcaa aagcggcaaa ggattcatta tcccctccga
    41641 cggtcgtaaa gaagttcagg tccatatttc cgcattcact ccccgcgacg cagaagtgct
    41701 tataccagga ttacgcgtgg aattttgccg cgtaaatggc ctgcgaggac caacagcggc
    41761 aaatgtttat ctctcttaat atgacgctgc ttgtttaaag ctgactggtt ttattggcat
    41821 agttcaatag tcaacaccat gcattataac acaagttact ttttcaccgg aaaactaatc
    41881 catttaaccg aatatctcta caactaatag ttataaatgc cacttaacag cgagttatag
    41941 taccacttga aactatattg ccaacaatag ttaatagctt tcttaacaat tttcttatct
    42001 ttaatttgta ttttgctaag atttatctta tgctcacagg tcgtatttcc agaggggaat
    42061 agttaacaat cttatatttc cctctgcgcg cctttctcac ccgctgtata tagtgcaagt
    42121 ggtatgagga aagggagatt tcacaaggat gttatactca aagtagacaa tcctgatctc
    42181 aagcgtacgt attgtccatg cgatgaataa aggaaaagtt atgaaacaca agctttctgc
    42241 aatccttatg gccttcatgt tgacgacgcc agctgcgttt gctgcccctg aagcagcaaa
    42301 cgggaccgag gcaacgacgg gtactactgg caccacaacg accactaccg gtgcaaccac
    42361 gacggctgct accactggtg gcgtggctgc tggcgctgtc ggcacggcga cagttgtcgg
    42421 tgtggcaacg gctgttggtg tggcgacgtt ggcagtagtt gcagcaaatg acagtggcga
    42481 tggcggttct cataacacct caaccaccac gtccactacg cgctaattct gcttaaacgc
    42541 gtcgaaatta aagttaaata atacccggtt gcgatattcg taccgggctt tagagatttg
    42601 cattttttta attaaccttt caaggattaa aggtgcgccc tcttatttta tcgattttcg
    42661 cactatttct tgcgggatgt acgcacagcc agcaaagtat ggtcgataca tttcgcgcca
    42721 gccttttcga taatcaggat atcaccgtag cggatcagca gatccaggcg ttgccttatt
    42781 ccacgatgta tttacgcctt aatgaagggc aacgaatctt tgtggtactg ggatatatag
    42841 aacaagaaca aagcaaatgg ttatcccagg ataacgccat gctggttacc cacaatggac
    42901 gtcttttaaa gaccgtcaaa cttaataata atctgctgga agtgacaaat tccgggcagg
    42961 accctctgcg taacgcgctg gcaataaaag acggcagccg ctggacgcgc gatattctct
    43021 ggagtgaaga caaccatttt cgctctgcga ccctgagttc cactttttcc tttgctggat
    43081 tagagacgct gcatattgcg ggtcgcgatg tgctgtgtaa tgtctggcag gaagaggtga
    43141 cttccacgct accagaaaaa cagtggcaaa acacattctg ggtcgattcg gctactggcc
    43201 aggttcgtca aagtcggcaa atgttaggcg caggggttat tcccgtagaa atgacgtttc
    43261 ttaaacccgc accatgaata aattacagtc gtatttcatt gccagcgtac tttacgtaat
    43321 gacaccccat gcctttgcgc aaggaacggt gactatttat ctgcctggcg aacaacagac
    43381 actttccgtt ggccccgtgg agaatgttgc ccagttagtg acacaaccgc aactgcgcga
    43441 tcgcctctgg tggcctggtg ccctgctgac cgactccgcg gtgaaagcta aagcgcttaa
    43501 agactaccaa catgtcatgg cacagcttgc ttcctgggaa gcagaagctg acgacgatgt
    43561 ggcggcaacg attaaatccg tgcgccagca gttactcaat ctcaatatta ctggtcgctt
    43621 accggtcaaa ctggaccctg attttgtacg agtggatgag aacagcaatc ccccgctggt
    43681 cgggaactat acgctctata ccgtacagcg cccggtgact attactcttc tgggtgcggt
    43741 ttctggggct gggcaattgc cctggcaggc aggccgtagc gttaccgatt atctgcaaga
    43801 tcacccccgt cttgcaggcg cggacaagaa taacgttatc gtgattacgc ctgagggtga
    43861 gactgtcgtc gcccctgttg ccctgtggaa taaaagacat gtggaaccac cgcttggcag
    43921 ccagctttgg ttaggctttt ctgcgcatgt actgcccgaa aaatatgctg atttaaatga
    43981 ccagattgtg tctgttttaa cgcaacgtgt tcctgactga tgaagaagaa ttcttatctt
    44041 ttaagctgcc tggccattgc cgtctccagt gcctgtcatg ctgaagtatt aacctacccg
    44101 gatccgctgg gttcgtcgca atcggacttt ggcggcacag gattgttgca gatgccaaat
    44161 gcgcgcatcg caccggaagg tgaattcagc gtcaactacc gggataacga tcaataccgt
    44221 ttctactcca cctccgtggc gctgttccca tggctggaag gcaccattcg ttatacggat
    44281 gtgcgcacac gtaaatatag ccagtgggaa gatttcagcg gcgatcagtc atacaaagac
    44341 aaatcattcg attttaaact tcgcctgtgg gaagaaggtt actggctacc gcaagtggcg
    44401 tttggtaaac gtgatattgc tggtacgggt ctgtttgacg gtgagtatct ggtggccagc
    44461 aagcaagcgg ggccatttga tttcaccctc gggatggcat ggggctacgc cggtaatgcg
    44521 ggcaatatta ccaacccgtt ttgccgggtg agcgataaat attgtcatcg cgcagagtct
    44581 cacgatgcgg gcgatatcag ctttagcgat atctttcgtg gcccggcttc catctttggc
    44641 ggcattgagt atcaaacgcc gtggaatccc ctgcgtctga aactcgaata cgacggcaac
    44701 aattaccaga atgatttcgc tggcaaactg cctcaggcaa gccatttcaa cgtcggtgca
    44761 gtttatcgcg ctgccagctg ggcagacctc aacctgagtt atgaacgcgg taacacgttg
    44821 atgtttggtt tcactttacg gaccaatttc aacgatctgc gccctgccct gcgcgatacg
    44881 ccaaaaccgg catatcaacc tgcgcctgaa tctgaaggat tgcagtacac cacggtagca
    44941 aaccaactta ccgccctgaa gtataacgcg ggctttgacg cgccagaaat tcagctacgc
    45001 gataagacgc tgtatatgtc tggtcagcaa tacaaatacc gtgactctcg tgaagcggtc
    45061 gatcgtgcca accggattct ggtgaataac ctgccgcaag gcgttgagaa gattagcgtg
    45121 acgcaaaagc gcgagcatat ggcgatggtg actaccgaaa ccgacgtagc cagcctgcgc
    45181 aaacagctgg caggtacagc gcctggtcaa tcagagccac tgcaacaaca acgtgttgaa
    45241 gctgaagatc tttctgcctt tggtcggggc taccgtattc gtgaagatcg ctttagctac
    45301 tctttcaacc caacactttc acagtcgctg ggcggcccgg aagatttcta tatgttccag
    45361 ctggggctga tgtccagcgc ccgctactgg tttaccgacc acctgctgct tgatggcggt
    45421 attttcacca atatttacaa caactacgac aagtttaagt cttcgctgtt gcccgcggac
    45481 tctaccctgc cccgcgtgcg cacgcatatc cgtgattacg ttcgcaatga cgtttatctc
    45541 aacaacttgc aagcgaatta ctttgccgac ttaggcaatg gtttctatgg ccaggtgtat
    45601 ggcggttatc tggaaacgat gtatgccggt gtcggttccg agctgcttta tcgcccgcta
    45661 gatgccagct gggcgctggg tgtggacgtt aactacgtga agcagcgtga ctgggacaac
    45721 atgatgcgct tcaccgatta ttccacgcca actggtttcg tgacggctta ctggaacccg
    45781 ccgacgctca atggcgtact gatgaaactt agcgttgggc aatatctggc aaaagataaa
    45841 ggggcaacga tcgacgtcgc caaacgcttt gacagcggcg tggcggtagg ggtatgggcg
    45901 gcaatcagta acgtatctaa agatgactac ggcgaaggcg gctttagtaa aggtttttat
    45961 atctcgattc cgttcgactt gatgaccatt ggacctaacc gcaaccgcgc ggtggtttcg
    46021 tggacaccat tgacgcgtga tggtggacaa atgctgtcac gcaaatacca gctctatcca
    46081 atgacggcag agcgagaagt accggttgga caataacctg acgtaattca taacattaca
    46141 ttgactgata aatataaacc aatgaaaaag aacattttta agttttcggt actgacattg
    46201 gctgttctgt ctttgacagc atgtaccctt gttcctggtc agaatttatc gaccagtaac
    46261 aaggatgtca ttgaactacc tgacaatcag tatgacctgg acaaaatggt caatatctat
    46321 cccgtcacgc caggattgat tgatcagttg cgcgccaaac cgattatgtc gcaggctaac
    46381 ccggagctgg agcagcaaat cgcgaactat gaatatcgta tcggcattgg tgatgtgttg
    46441 atggttacgg tctgggatca cccggaacta accacccccg cagggcaata ccgtagcgcc
    46501 agtgataccg gcaactgggt taacgccgat ggtgcgattt tctaccccta tattggtcgc
    46561 ctgaaagtgg ccgggaaaac acttacccag gtacgcaacg agattacagc gcgtctggat
    46621 tcagtcattg aaagcccgca agtcgatgtc agcgtcgcgg cgttccgctc gcaaaaagcg
    46681 tacgtcaccg gtgaagtcag caaatcgggt cagcagccga ttaccaatat tcctctgacc
    46741 atcatggatg cgattaacgc cgcgggcggt ctgaccgcag atgcagactg gcgcaatgtg
    46801 gtgctcaccc agaatggcgt aaagaccaaa gtgaacctct atgcgctgat gcagcgtggc
    46861 gacttgcgcc agaacaaact gctgcacccg ggagatattt tatttattcc gcgcaacgat
    46921 gatctgaaag tcttcgtgat gggtgaagtg ggtaaacaga gcacgctgaa aatggaccgc
    46981 agcggtatga ccctggcgga agcgctgggt aacgctgagg gtatgaatca ggatgtcgct
    47041 gatgcgacgg ggatttttgt tattcgtgca acgcaaaaca agcagaatgg caaaatcgcc
    47101 aatatttatc agctgaatgc gaaggacgca tcggcaatga tactcggaac ggaattccag
    47161 cttgaacctt acgacattgt ttatgtcact accgcgcctc tggctcgttg gaaccgtgtg
    47221 atttccctgc tggtaccgac gatttccggg gtgcatgacc tgacagagac ttcacggtgg
    47281 attcaaacat ggcccaacta aaatttaact caatcctggt ggtttgtacc ggcaacattt
    47341 gccgttcgcc cattggcgag cgcttgctgc gtaaacgtct gccgggcgta aaagtcaaat
    47401 cggcaggtgt tcatggtctg gtaaaacacc ctgcagatgc gacagcggca gatgttgccg
    47461 ctaatcatgg cgtatctctt gaaggacatg ccggacgtaa gctcactgct gagatggcac
    47521 gaaattacga tctgattctg gcaatggagt cggaacatat tgctcaggtt accgcaatcg
    47581 ctcccgaggt ccgcgggaag acaatgctgt ttgggcaatg gctggaacag aaagagatcc
    47641 cggatcccta tcgtaaaagt caggatgcat ttgaacatgt ctacggaatg ttggagcgcg
    47701 ccagtcagga atgggcgaag cgcctcagcc ggtaatttga gtttataaaa tacgatgaca
    47761 actaaaaata tgaatacgcc accaggcagc actcaggaaa atgagatcga tctgcttcgt
    47821 ctggtcggcg agttatggga tcaccgtaag tttattatca gcgtgaccgc gttattcacg
    47881 ctgatcgctg tcgcttactc gctgttaagc acaccaattt atcaggcaga tactctggtc
    47941 caggttgagc aaaaacaggg caacgccatt ctcagcggcc tgagtgatat gatccctaac
    48001 tcatcgcccg agtctgcacc ggagatccaa ctgctgcaat cgcgcatgat tctcggtaaa
    48061 accattgctg aactgaatct gcgcgacatg gttgagcaga agtattttcc gattgtgggt
    48121 cgcggctggg cgagattaac caaagagaaa ccaggtgagc tggcgatcag ctggatgcat
    48181 attccacaac tgaatggtca ggatcagcaa ctgacactca cggttgggga aaacggccac
    48241 tatacgctgg aaggtgaaga gttcaccgtc aatggtatgg tcggccagcg tctggaaaaa
    48301 gatggcgttg cgctgactat cgcggatatt aaggccaaac caggaacaca gtttgtcctg
    48361 agccaacgta ccgaactgga agcgattaac gcgttgcagg aaacctttac cgttagcgaa
    48421 cgcagtaaag aaagcgggat gctggaactt accatgactg gtgatgatcc ccagttgatt
    48481 actcgtattc tgaacagcat cgctaacaac tatttgcaac agaatatcgc tcgccaggcg
    48541 gcgcaggatt cacaaagcct tgaattctta cagcgccagt tgccagaagt gcgcagcgag
    48601 ctggaccagg cggaagaaaa actcaacgtt tatcgccagc agcgcgattc ggttgacctt
    48661 aacctggaag ccaaagccgt tcttgagcag attgtgaacg ttgataatca actcaatgag
    48721 ctgaccttcc gcgaggcaga gatctcccag ctgtataaga aagatcaccc aacttatcgt
    48781 gcgctgctgg aaaaacgcca gacgctggag caagaacgca aacgcctgaa taagcgggta
    48841 tcggctatgc cttccaccca acaggaagtg ttgcgtttaa gtcgtgacgt agaagcgggc
    48901 cgtgcggtat atctgcaatt acttaaccgc cagcaggagt tgagtatttc gaaatccagt
    48961 gccattggta acgtgcggat tatcgacccg gcagtcactc agccgcagcc agtgaaaccg
    49021 aaaaaagcgt tgaatgtggt gcttggtttt attcttggcc tgtttatttc ggtgggtgcc
    49081 gtgctggcgc gtgcgatgtt gcgtcgtggt gtagaagccc cggaacaact ggaagagcac
    49141 ggcatcagcg tttatgccac catcccgatg tccgagtggc tggataaacg tacccgtctg
    49201 cgtaagaaaa atttattttc taatcagcag cgccatcgta ctaaaaatat ccccttcctg
    49261 gcggtggata acccggcgga ttctgctgtg gaagccgtac gtgcgctacg aaccagtctg
    49321 catttcgcta tgatggagac tgagaataac attctgatga tcaccggtgc gacgccagac
    49381 agtggtaaaa cgtttgtcag ttcaactctg gcagcggtga tcgcccagtc cgatcaaaaa
    49441 gtgttattta ttgatgccga cttacgccgt ggttattcgc ataacctgtt taccgtgagt
    49501 aatgaacatg gcttgtcgga atatctggca ggtaaagatg agctcaacaa agtgatccag
    49561 cattttggca aaggaggctt tgatgtgatt actcgcggtc aggtgccacc taacccatct
    49621 gaactgctga tgcgcgatcg gatgcgtcaa ttactggaat gggcgaacga ccattacgat
    49681 ctggtgattg ttgatacgcc gccgatgctg gcggtgagcg atgctgcggt cgtggggcgc
    49741 tctgtcggca ccagcctgct ggttgcgcgt tttggcttga acaccgccaa agaggtgagc
    49801 ttgtcaatgc agcgtctgga acaggctggc gttaatatta aaggcgcaat cctcaatggt
    49861 gtgattaaac gcgccagcac cgcttacagt tacggctata actattacgg ttatagttac
    49921 tccgagaaag agtaaaactg aaggtaaagt gaccggatgc ggcgtgaacg ccttttccgg
    49981 tctacgcatt agacagttct tcgtttgtca tcagcctcag agcattcagg tagctgaatg
    50041 ctctttttta tacattacaa actgcacgcc ggtatgcgtg cttcattcac gatttgcgta
    50101 aaaccggcca acgaacacat gccctgcgca tttcgctctt cacatcctgc cagggtcagt
    50161 ttcacctctc cgggcggcgt atttaatgac agcggagttt tatcacgcat ctgctgtaaa
    50221 gtctggaaga ccagcgaaac ctgaatccac tggctgttat cgcttagccg acgccagcgt
    50281 tcaaacacca gttcaccacc tggcggcgtg ttatccggct gacctggaag cgtccagttg
    50341 agctccagtg cgccgccgag atttgccaga ttagtatcgt gtccggcgat aaacagcact
    50401 gaagtgggta atgtcacacc atacgcctgt ttttgcggtg gatggggagt caacgctatc
    50461 atgatcaaat ccaacaacgg ggtggcgcgg ctgcgggcaa cctctggcgt gcgttgtagt
    50521 aaataaaatt gcgcgttatg caaacttagc aacgtgttcc actggtgtga atcagtgatc
    50581 cttccccacc ccggctccgg cattccctgt gcctgttgca ggagaaatat ttccgtcagc
    50641 attgatgcga ggcttaccgc accggttaat gagacattgt cggcgctcac cttgagttcc
    50701 gatggtaatg cctgcgttaa tgaacagctt tcgtcctgtt tctcacggtt aaggcacaag
    50761 tttgattgcg gaaaattaag cacccgttcc agttcgcgaa acgccgtttg ccgatgcccg
    50821 gtaaagtcag caattgaccc tcctgccctg ctgaggatcg cgtcagtcac gttcgcgtta
    50881 tccagttggc aaacgccagt ttttagagga ttaaataacg gatcgggact ggacgtatct
    50941 gcctgggtat gtacggttat tgcacagtca ggtgccagcc cggcggcgaa ggcttcgcct
    51001 gttttacggg tacgctcgtc gacatcagca ataatcgcga cctgaccagg ctgcgggcag
    51061 ccctttttcg tcagcaatcc gtcggctacc agacgctggc gttggtaatg tccgagataa
    51121 gcgattagct caccaccgcg cggtgtcagc caacccagtt ttaccggcca gtttggccat
    51181 gcgtctgggg tgacatcctg catcagttgc gtggccttgg ttggggcacg cacaccatga
    51241 cgactgacaa tcaccacact ttccagcttc agctccggct ccggctcact ctgagcgaat
    51301 gcagattgcg gggttaacgg aatcagaaga gataaaaatg ggatcaagat cgctttcatc
    51361 gatatgtttc cgcttttcat atccgacatt attgattgcc tgatacgacg ctaatgcgcc
    51421 gcatcagact tacgttcgtt aacttttcaa tcgcgggtcc agccatacca acacaagggt
    51481 ggagagcgaa cacatcaaca aaatgccgac gaaccaaagt aaataccaca ttgttcctgc
    51541 tccttagtac agctcgtttt cgttacggcg gagagtttct gtcgtcatgc gcccccacat
    51601 tttgtagtag ctccagagag tgtagagcaa cacaatgggc aaaaatatca gcacgattac
    51661 caacataata ctcagcgtca gctgactgga agtgctgtcc cacaacgtca ggctggagat
    51721 tggactcacg cttgacggca tgacaaaggg gaacagcgtg atgcctgccg tgaaaatcac
    51781 gccaaattgc atcaatgacg ccatcaaaaa tccccaaccc gggcggccac gataaatcgc
    51841 catcaccgtc agcaatgggc agaagaaccc cagcagcggg aagatccaca aaacgggcga
    51901 ctcgacaaaa ttattcatcc aggcaccagg tagcactgcc accagtttca ttaacggatt
    51961 ggaaggaccg ttagcatcct gggcgagcag tacaaagcca tcaataccga cccacagcca
    52021 gtaacccgcc agcaaaaagc acagcatcac caacagtgca gcgcgtttag tcgacagctg
    52081 tgaacgcaga tgaatcacac caacagtttt cagttgtaac cagacgccac cttgcaaaat
    52141 caccatccca aggctgagca atccgcacaa taaagggaat ggcgtcagca gttgccagaa
    52201 gctgccgaga tactccacgc gtaattgcgg agtgaaggca aacggcacgc cgagcaacaa
    52261 gttgccgaag gcgataccga agactaccgg cggcaccaga ctgccgatga ccagaccggc
    52321 atcccacatt ttacgccagc gtgcatcggc gatttttccg cgataatcaa aggccagcgg
    52381 gcggaagaac agcgagcaca gcaccaggat catcgccaca taaaagccgg aaaacgccgc
    52441 tgcatacact ctgggccagg cggcaaataa tgccccacct gcgaggatca accagacctg
    52501 attgccttcc cagtgcgcac caacgctatt aatcactatc cggcgttcat catcatcacg
    52561 cgccaccagc ggcagcaaac agccaatccc catgtcaaat ccgtcggaga tcataaagac
    52621 caccaggatc acgccaatca gcagccacca gatgaagcgc aatgtttcat aatcaaacat
    52681 gattttctcc tttacccctg ttgctgcgtc ggttgttcac tctgcatcgc gctcggcccc
    52741 agacgggcat atttctgcat caggtagact tcggcgatta agaacagggt gtaaagccct
    52801 acgatcatga tcagtgagaa agccagttgt cctgtggtta aagcggagtg cgcggagtat
    52861 gtcggtaaga tgtcctgtat cgcccacggc tgacgaccaa actcggtcat aaaccacccg
    52921 gcttcaatcg caatccacgg caacggcaaa ctccagagcg ccattttcag cacccagcga
    52981 tgctggtcga ttttgccacg cagcgtctgg acaagcgcaa tcagcatcac cagtagcagc
    53041 agggaaccac agcccaccat gatgcggaaa ctccagaata ccggcgcaac ctgaggtatc
    53101 gcgccacgca tcgccgcctg gtactgtgcg gctgtgacat gattcatatc cggcgcatag
    53161 cgggagagca acatgccgta gcccaggtcg ccttccagct cccggaatgc ctgcaacaca
    53221 tgcggctcac gattgccttg cgatatttcc tgcattaaca gccaggccat acgtccgcgt
    53281 tgcaagcgtg ggtaggtttc agccatcaaa ttcttcagac ccggcacggg tttatctaat
    53341 gagtgagtgg cgaggatccc taacagcacg ggaattttga tggcaaaggc gttacgctct
    53401 tgatcctgtt ccggccaggc aaccacatgg aacggtgcag gagcaggttc cgtttgccac
    53461 tccccttcca tcgccgccag ttttaccggt tgtacttgcg cgacttcata cgcagaactg
    53521 tctccgagtt gcagggtacc gataatcgcc agagtaccga agacggaacc gatggcaaac
    53581 gagcgtaatg cgacatcgcg ctcccgtccg cgcagtaaat accaggcgct gatcgccata
    53641 ataaacatgg ccccggtcac gtagcccgcc attacggtgt gcacaaattt cacctggctg
    53701 accgggttaa agaccagctc gctgaagctg gtcatctcca tacgcagggt gtcgatatca
    53761 aaatgcgcac cggtcgggta ttgcatccaa ccgttggcat tcaatatcca caatgcagag
    53821 agatttgaac cgaacgccac cagccacgtc accagcaggt gctggtattt attcagccgc
    53881 tgccagccga agaagaaaag cccgacaaag gtggattcga ggaagaaggc cattaatgct
    53941 tccatcgcca gcggtgcgcc aaaaatatcg cccacatagt tggaatagaa tgaccagtta
    54001 gtaccaaact gaaactccat ggtcaggccg gtagccacgc caagagcaaa gttgataccg
    54061 aagagcttac cccagaagcg cgtcatatcg cggtagattg ttttgccggt gaccacgtaa
    54121 atggtttcca tgatagccag caaaaaaatc agccccaggg taaggggtac aaataaaaag
    54181 tgatacagcg cggtcagagc aaactgccag cgcgataaat caatgacatc ccacatgcgc
    54241 actcctgtag gcgaagagag aaaaataacc cgttaatggt agatctccct ctttatcctg
    54301 aaactctacg accagaggta ttcgccgatt gcgatattca taagcctgaa agcgatactt
    54361 accgtctttt tacgtcggtg cagcttccgc cagccactga catacctcac taagccgctg
    54421 cgcagagtcg accaaatctt ctggcgctgc cagcaccact tccggtattg ggcagatttc
    54481 ataactttcc agtaacgggc cttttaaggt gtccgtacag cgtaaatgcc agacatggcg
    54541 taaccccgtg gcgttgatat agctctcgcc atagccaatg gtacgaatct gaatatttcc
    54601 cggcccacag agacgtgaga gaaaaagccg atcagcctcg ctgatgggta attgcgtcag
    54661 attaatgctg tgcggctgcg cgtcggggtt acgtacatgc gccagtaact catgcgccaa
    54721 cggtaggcca ttcatcaggc catcaatggg cggcggtaac agcgaatctg tcggcaagag
    54781 attttgcgtt gccgcctgcc acaacgccag cggcgcgcag ccagcctcca gtttgtcctc
    54841 cagcaacttt tcgccgcgac gtctgcgcac ccgccataat ccgcagaaga tcgcctcctg
    54901 tatttcactt tcgctgtcgt cagcctgctg aatgcgcact gacacttcac cttccccgag
    54961 aagcgtattg ataaagtgac gatcgtcggc gttgagattt aacagatcga ctgccagcac
    55021 ctcgcccaac ggcggctggc gttgacttag ttgatgttgt aactcgttta acagcgcaat
    55081 cacctgcggg ctgtgagcac attgctccag ggccgccata ctcggttcat cattcacctg
    55141 acaggtgatc ggcagtggat tcatgctgaa actgtcatcg ttcggttgcg ttcttggccc
    55201 cagcagatgg aaaaaagttt cgctcatgag gcacgctcct gctgtggttc gacaagccca
    55261 cgcatcaggt ttatcagttc cgcccacggg tgaataccat tcagcacgcc gcgatagttt
    55321 ccgccggtaa acactaatgt ggcgggaaag cgaaagacgc caaagcgatc tccgatggct
    55381 tcgctctgtt caaggtcagc aatcgccacc tgccatgtgt agtcgggaaa ctcgcgcagt
    55441 aattcgccaa tcattaccgg attatcgctg acctctggcg tgcgtttcgg gtcactgctt
    55501 aataacacca cgccgtctgg cgcttgcgta agccagtcgt caagacggaa ttcactaacc
    55561 ggcgtccagc cgcgcgccag cattcgttgc cacaacgcat caaatggcgt gtcgttgctc
    55621 atccctgctc ctccagtgtc atcctgtact ggcgcaagcg aacgccttcg taattttcca
    55681 tcgacagaca gtcataattg agacagcgcg attcattagc cggttgcggc acaattcccc
    55741 atgcagccag ctgcgccagc gccgcctgtt ccgctgcggg cagttgctcc cgtgccagtt
    55801 cgctcaggct gccgccgtag tcgtcgagca ttgcgggttg cagaccgacg agggcaatat
    55861 gtgctggcag atgtccgcgg atatctgcca gcgccagcac ttcggagaaa ctgttctggt
    55921 gcaggctcat tttcttcgcg ctgagataag ccggaatacg ttctccggca taggttcgca
    55981 gcgttcctgg ttccagcccg tagtcaatgg catcgagaat caacagatgg ctggcgcttt
    56041 cgacataccc cagcaagttc agtccctgag taccgccatc gacaatctcc acagactcgg
    56101 gccagtggta atgggcatac aaccgttccg ccacccgcac gccgaaacct tcatcggccc
    56161 acagcaggtt gcccagcccc attaccacca cgcgttgctc gctcatgaac gctccttgtt
    56221 acttattttg ccaaatttgt ggctacggta gccgttgacc atggtggaga tcaccgtgtc
    56281 gtcggacatg atgtcttcac gcagcgccat atagacatga ccgatcacaa acgcgccaat
    56341 cagccacatc cccagccgat gccagctgtg aatgtccatt gagttgcctc ccgtccagta
    56401 gaaaaattcc accacataac ggaacggcgc aaaaatagcg tactggctgt gttcgctgta
    56461 cagcgcaaag ccggtgatga tcataaagac cgacatcagg aaatagccaa acatcgccgc
    56521 ctgggcgatg gggttatggc ctatatcggc actcggacgt tttgccagaa acagatacca
    56581 gcggatttca taccacacgc cctgccacca gcttttacgc catactggca cgataaacag
    56641 ctcgcgggag tatcgattgc caacaaaagc ccagtagatc cgcatcagca agaccacggt
    56701 aaaaaccatc ccggcgctga agtgaattaa cctgatgtag cccatataga acagatacgt
    56761 cgcctcgccg ctgacggaag gtagcggttt gccgataaag taaccggtga ccatcaacac
    56821 cgccatgcat aacaccgtca gccagtgcca gatacgcact ggcgcttcaa agacatagtg
    56881 gctgacaacg ttgtcgcttt tctgttgcat gatgattctc cttcgctgtt aacgcacctg
    56941 cacggagatc agctcgctac cgtcgtcgcc cagcacgtgt gttgaacagg cgaggcacgg
    57001 atcaaagctg tgcagagtac gcaggatctc cagcggttgc tcggggatcg ccattttggt
    57061 gttcatcagc gccgcttcat aagcgccaat ctgccctttg ggatcgcgcg ggctggcgtt
    57121 ccaggtggtc ggcaccacgc actggtagag atcaatcttg ccatcgcgaa tggcggccca
    57181 gtggcctaac gccccgcgcg gcgcttcggt aaaaccgaca ccacggcact ctgtcggcca
    57241 ggttgtgggt tcccattttt ccgtggaggc ggtggcaaga ttgccgtttt tcaggttagt
    57301 catcagcttg ttgaagaaat actgcaactt acctgcggcc cactgtgctt cgtgcgcgcg
    57361 gcacaaaatg cggcccaacg ttgactggat accggaaagc ggcaggttca gcgccgacat
    57421 catgcgatcg accgactcaa cggtcgcagc atcgcctttg tgataagcga ttaacgtgcg
    57481 tgccagcggc cccacttcca tcgcgttacc gcgccagcgc ggcgctttga tccacgagta
    57541 gcgttcctgt tcattcagct gctgaatgtt ggtatcgctg cctttgacat cgccggggtt
    57601 gtaccacggg tcggtgatgc catcgaacgg atgacgcccg acctgatcgt tgggataacg
    57661 ataccaggcg tgatcgacaa actcctgcac ctgctgcgga tcaaccaaat ccactggcag
    57721 cacattgttg aagtcgccgt taatcaccgc gccgccaggc atcagcagac ttttctcacc
    57781 aaagtcgttg gcaatatccg ggaatgcgcc gtagctgagg acacatttat cagaaagacc
    57841 cgtgccgatt tcgctccacg gtttgttgaa ctgaccgatg gctaaggcgt cggggatcat
    57901 cacgttgtta atgaagtctg ccgtgcgggt gataatcgac tgcaccaggt tcaggcgttc
    57961 catattgacc gccccgaccg cgccgctttc gtcaatgtta atggcgcaag gcatcccgcc
    58021 gacaatccag tttggatgcg ggtttttacc gccaaagacc gcgtggattt tgacaatttc
    58081 acgctggaaa tcgagagctt cgagatagtg ggcaaagccc atcaggttgg cttctggcgg
    58141 caacttgtac tgcgggtgcc cccagtagcc attgcggaag atccccaact gcccgccttc
    58201 aacgaatttc ttcaggcggt tttgtacgtc gaagaaatag ccaggggatg atttcggcca
    58261 agaggagaga ctttgcgcca gttcggaggt tttccgcggg tcggctttca gcgcatccaa
    58321 cacatcgatc cagtccatcc cggcaagctg atagaagtgc accagatgat cgtggcacca
    58381 gagcgttgcc agcataatgt tgcggatgat attggcgttg tccggcactt taataccgat
    58441 ggcatcttcg atggcgtaaa ccgaagccag agcgtgtacg ccagtacaga cgccgcagat
    58501 acgttcaacg aacgcccacg catcgcgcgg gtcgcgccct tgcaggatga tctccagccc
    58561 gcgaaacatg gtgccgcagg agacggcatt ggtgattaca ttctgatcgt taatattcac
    58621 ttcgcagcgc atgtggcctt caatgcgcgt aatcgggtcg accaccaggc ggcgtccggc
    58681 attattgatg gtgtatccct gagtttcgta ctgagtgctc atgcctgttt atcctcattg
    58741 cctggctgat gttcggtttc tgtaggttgc tgattatgac gtctgcgctg gtcaacggcg
    58801 ctggcgactg cgtgcacacc aacagccgct gccaccacac caagcgcggt taaaccgacg
    58861 gtatcggcgg tggaatgagt acccatttgt ggaatatcga ccacgcggct gtagaacgaa
    58921 ccgcgatccc agaaaccatt ttccgcacag cccaggcagc cgtgaccaga ctggattggg
    58981 aaagaaacgc catcattcca gcgtgtggag gaacaggcgt tataggtggt aggccctttg
    59041 cagcccattt tgtacaggca ataacctttg cgggcagcgt catcatccca actctggacg
    59101 aactctccgg cgtcgaagtg agcgcggcga tagcatttat cgtggatacg ctgaccataa
    59161 aacatcagtg gacgacccat tctgtcgaca tctggcaagc gatcaaaggt caccatgtaa
    59221 gtaatgatgg cgctcatcac atccgggatc ggcgggcagc caggtacttt gataatgggt
    59281 ttgtcggtga tgactttgtc gatagacgtt gcctgtgtcg gattaggtcg cgcggcctgc
    59341 acgcagcccc aggacgcaca ggttccccag gcgataatcg cgctggctcc ggcagcggca
    59401 cgtttgagtt tctcaataaa cggtcgaccg ctgctgatac agaacatccc ctgctcgccc
    59461 agcggcggat taccttctac tgcgaggata tatttgccat tgtattgcgt gatgatgtct
    59521 tcaaagactt cttccgcctg ggttccggcg gcagccatca aagtatcgtc gtaatcgagg
    59581 gaaatcaggg aaaggatgac gtcctttgcc agcgggtgag cggagcggat aaaagattcg
    59641 gtacagcagg tgcattccag accgtggatc cataccaccg gaatgcgcgg tttgttctcc
    59701 agcgcccagg caatctttgg tgccattccc gcgcctaatc ccagcgacgt ggcagccaga
    59761 ctacaatatt tgagaaagct gcgccgggta acgccctgac gccgcatggc ctggtaaaat
    59821 gtttcctcgt tattcatatc gcacgtctct tctccttgcg acaccggcag gacaccgggc
    59881 gcttatggtc ggcaagcgac gtccctgtag caaggcaaac catcgcttca cgtgagatgc
    59941 tgaaaacgaa agctcatcct tctgcactgg cgcacgtcgc cagaaagtat tgttaataaa
    60001 gcgtagtgaa acttttgcac aaaacaatac aaactgtgtg gatttatctt ttagcgataa
    60061 aaatggacct atttttcttt ttgccgggcg gagggatgtt tagccagttg ctaaacgagt
    60121 aaaagagaag gaatttatgg tgatgaaatg atcaatctgc ttttatttca cgctatcaat
    60181 gcacttttgg ctgtttttca ggcaaacaaa cagattaggg gtttacacgc cgcatcggga
    60241 tgtttatagt gcgcgtcatt ccggaagtgt ggccgagcgg ttgaaggcac cggtcttgaa
    60301 aaccggcgac ccgaaagggt tccagagttc gaatctctgc gcttccgcca aataatcaag
    60361 gggttaccaa atggtagccc ctttgctttt ttggctcttg gtataaactt ggtataccaa
    60421 ttggtatatt tttattcgga tgagattatc ggtcatgagc ggaacctcag gttcctcgtc
    60481 tgatgccgct cttgcaacac gttacgctgc cgagtatttt tgtaaaacat ggactgctcc
    60541 aggcctgaac caggcagaag gatacaaagc catctcagat ctgtcgcatc actattttcg
    60601 tgcagaaggc tctagccctc cccaatcttt tttgtgacac cctgtttcac tgaacatgaa
    60661 gacactcacc ttgtccctga tattcattag catcatcctg attatttaac ttctctattt
    60721 agggaagccc gtctgataca aagcttctat tcctcgttaa atattctcca ttctggcgcc
    60781 aagccctatc tatagctgat tgaaaacaac tctaaaaacg taataatatc aaatagttta
    60841 ttcctacagc cgatcatttt gagtaaaata ttgacgagaa tttcacaaaa ggtagatttc
    60901 atgtaaacgg ataaataaga ctatccaata aagctcttat aaggttatct atatgataaa
    60961 tagcattaat tctttttttc cggccttcct cgcagtatat caagtgctat acgcagcagt
    61021 accttcactg tctcgcagca caaaagcaca cctaacacgg taaaaaccag ctcacctttt
    61081 tctcccagca acagtcctgc cagtgcaaca actattttta aggtaaaaaa ttcttataca
    61141 gaatcaggac ttcaacgtcc cacatcttat actcagtcaa gtatagaaaa aaacgcttta
    61201 catcggcctc tacctgatgt
//
LOCUS       DAZFAB010000018        59773 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZFAB010000018 DAZFAB010000000
VERSION     DAZFAB010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 59773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59773
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.018"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..650
                     /locus_tag="ACHJWA_001293"
     CDS             <1..650
                     /locus_tag="ACHJWA_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFO4307933.1"
                     /translation="GIWIGSAISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFT
                     LTIIQTIAETGKEGMVMPSEIGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAG
                     ISEPALYGVAIRLKRPLIASLISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANP
                     MSIVWVFAVMALAVVLSFILTLLLGFEDIPVEEAAAEARKHQSAQPTVAKEVSLN"
     gene            659..2083
                     /gene="ascB"
                     /locus_tag="ACHJWA_001294"
     CDS             659..2083
                     /gene="ascB"
                     /locus_tag="ACHJWA_001294"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFO4307934.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATGFYHRYKEDIALMAEMGFKVFRTSIAWSRPFPQGD
                     ELTPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMQLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNTNNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVFVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(2207..2677)
                     /gene="hycI"
                     /locus_tag="ACHJWA_001295"
     CDS             complement(2207..2677)
                     /gene="hycI"
                     /locus_tag="ACHJWA_001295"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFO4307935.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(2670..3080)
                     /gene="hycH"
                     /locus_tag="ACHJWA_001296"
     CDS             complement(2670..3080)
                     /gene="hycH"
                     /locus_tag="ACHJWA_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFO4307936.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(3077..3844)
                     /gene="hycG"
                     /locus_tag="ACHJWA_001297"
     CDS             complement(3077..3844)
                     /gene="hycG"
                     /locus_tag="ACHJWA_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFO4307937.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(3844..4386)
                     /gene="hycF"
                     /locus_tag="ACHJWA_001298"
     CDS             complement(3844..4386)
                     /gene="hycF"
                     /locus_tag="ACHJWA_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFO4307938.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(4396..6105)
                     /gene="hycE"
                     /locus_tag="ACHJWA_001299"
     CDS             complement(4396..6105)
                     /gene="hycE"
                     /locus_tag="ACHJWA_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFO4307939.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(6123..7046)
                     /gene="hycD"
                     /locus_tag="ACHJWA_001300"
     CDS             complement(6123..7046)
                     /gene="hycD"
                     /locus_tag="ACHJWA_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFO4307940.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVASSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(7049..8875)
                     /gene="hycC"
                     /locus_tag="ACHJWA_001301"
     CDS             complement(7049..8875)
                     /gene="hycC"
                     /locus_tag="ACHJWA_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFO4307941.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLSVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(8872..9483)
                     /gene="hycB"
                     /locus_tag="ACHJWA_001302"
     CDS             complement(8872..9483)
                     /gene="hycB"
                     /locus_tag="ACHJWA_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFO4307942.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(9608..10069)
                     /gene="hycA"
                     /locus_tag="ACHJWA_001303"
     CDS             complement(9608..10069)
                     /gene="hycA"
                     /locus_tag="ACHJWA_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFO4307943.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            10281..10631
                     /gene="hypA"
                     /locus_tag="ACHJWA_001304"
     CDS             10281..10631
                     /gene="hypA"
                     /locus_tag="ACHJWA_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO4307944.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            10635..11507
                     /gene="hypB"
                     /locus_tag="ACHJWA_001305"
     CDS             10635..11507
                     /gene="hypB"
                     /locus_tag="ACHJWA_001305"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFO4307945.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAC
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            11498..11770
                     /gene="hypC"
                     /locus_tag="ACHJWA_001306"
     CDS             11498..11770
                     /gene="hypC"
                     /locus_tag="ACHJWA_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFO4307946.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            11770..12891
                     /gene="hypD"
                     /locus_tag="ACHJWA_001307"
     CDS             11770..12891
                     /gene="hypD"
                     /locus_tag="ACHJWA_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFO4307947.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     QRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            12888..13898
                     /gene="hypE"
                     /locus_tag="ACHJWA_001308"
     CDS             12888..13898
                     /gene="hypE"
                     /locus_tag="ACHJWA_001308"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFO4307948.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPTNIHWG
                     AQTLTAGDILLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            13972..16050
                     /gene="flhA"
                     /locus_tag="ACHJWA_001309"
     CDS             13972..16050
                     /gene="flhA"
                     /locus_tag="ACHJWA_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFO4307949.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(16087..16440)
                     /gene="ygbA"
                     /locus_tag="ACHJWA_001310"
     CDS             complement(16087..16440)
                     /gene="ygbA"
                     /locus_tag="ACHJWA_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFO4307950.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKCP
                     VPELPEKYRPKKPRE"
     gene            complement(16517..16651)
                     /locus_tag="ACHJWA_001311"
     CDS             complement(16517..16651)
                     /locus_tag="ACHJWA_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4307951.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSVG
                     "
     gene            16727..19288
                     /gene="mutS"
                     /locus_tag="ACHJWA_001312"
     CDS             16727..19288
                     /gene="mutS"
                     /locus_tag="ACHJWA_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFO4307952.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTRLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            19394..20050
                     /gene="pphB"
                     /locus_tag="ACHJWA_001313"
     CDS             19394..20050
                     /gene="pphB"
                     /locus_tag="ACHJWA_001313"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO4307953.1"
                     /translation="MPSTRYQKINTHHYRHIWGVGDIHGEYQLLQSRLHQLSFFPETD
                     LLISVGDNIDRGPESLNVLRLLNQPWFISVKGNHEAMALDAFATGDGNMWLASGGDWF
                     FDLNDSEQKEATDLLLKFHHLPHIIEITNDNIKYVIAHADYPGSEYLFGKEIAESELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIK
                     "
     gene            complement(20091..20327)
                     /locus_tag="ACHJWA_001314"
     CDS             complement(20091..20327)
                     /locus_tag="ACHJWA_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit D"
                     /protein_id="HFO4307954.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWTVYQCQHCLYTWRDTEPLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLAEGKR"
     gene            complement(20338..21765)
                     /locus_tag="ACHJWA_001315"
     CDS             complement(20338..21765)
                     /locus_tag="ACHJWA_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit C"
                     /protein_id="HFO4307955.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFTDARVAMNTIGSWQNHAISLGLPPNTPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGDEINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRTRPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTAMLAARK"
     gene            complement(21765..22358)
                     /locus_tag="ACHJWA_001316"
     CDS             complement(21765..22358)
                     /locus_tag="ACHJWA_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HFO4307956.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAATISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPYARRWQGLPQARNFSQENE"
     gene            22505..22912
                     /locus_tag="ACHJWA_001317"
     CDS             22505..22912
                     /locus_tag="ACHJWA_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFO4307957.1"
                     /translation="MTLRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDAADKRRRFVWLTAEGEKILAAA
                     IPIGDSVDEEFLGRLSAEEQELFVQLVRKMMNT"
     gene            complement(23032..24024)
                     /gene="rpoS"
                     /locus_tag="ACHJWA_001318"
     CDS             complement(23032..24024)
                     /gene="rpoS"
                     /locus_tag="ACHJWA_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFO4307958.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(24087..25181)
                     /gene="nlpD"
                     /locus_tag="ACHJWA_001319"
     CDS             complement(24087..25181)
                     /gene="nlpD"
                     /locus_tag="ACHJWA_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFO4307959.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPAHTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(25366..25992)
                     /gene="pcm"
                     /locus_tag="ACHJWA_001320"
     CDS             complement(25366..25992)
                     /gene="pcm"
                     /locus_tag="ACHJWA_001320"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFO4307960.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(25986..26747)
                     /gene="surE"
                     /locus_tag="ACHJWA_001321"
     CDS             complement(25986..26747)
                     /gene="surE"
                     /locus_tag="ACHJWA_001321"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFO4307961.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(26728..27777)
                     /gene="truD"
                     /locus_tag="ACHJWA_001322"
     CDS             complement(26728..27777)
                     /gene="truD"
                     /locus_tag="ACHJWA_001322"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFO4307962.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELVELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(27774..28253)
                     /gene="ispF"
                     /locus_tag="ACHJWA_001323"
     CDS             complement(27774..28253)
                     /gene="ispF"
                     /locus_tag="ACHJWA_001323"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFO4307963.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(28253..28963)
                     /gene="ispD"
                     /locus_tag="ACHJWA_001324"
     CDS             complement(28253..28963)
                     /gene="ispD"
                     /locus_tag="ACHJWA_001324"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFO4307964.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVYA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGAAITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(28982..29293)
                     /gene="ftsB"
                     /locus_tag="ACHJWA_001325"
     CDS             complement(28982..29293)
                     /gene="ftsB"
                     /locus_tag="ACHJWA_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFO4307965.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(29487..29810)
                     /gene="ygbE"
                     /locus_tag="ACHJWA_001326"
     CDS             complement(29487..29810)
                     /gene="ygbE"
                     /locus_tag="ACHJWA_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFO4307966.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLMTVGIMFGALF
                     MWLLG"
     gene            complement(29860..30465)
                     /gene="cysC"
                     /locus_tag="ACHJWA_001327"
     CDS             complement(29860..30465)
                     /gene="cysC"
                     /locus_tag="ACHJWA_001327"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFO4307967.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(30465..31892)
                     /gene="cysN"
                     /locus_tag="ACHJWA_001328"
     CDS             complement(30465..31892)
                     /gene="cysN"
                     /locus_tag="ACHJWA_001328"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFO4307968.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRSRVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGEK"
     gene            complement(31894..32802)
                     /gene="cysD"
                     /locus_tag="ACHJWA_001329"
     CDS             complement(31894..32802)
                     /gene="cysD"
                     /locus_tag="ACHJWA_001329"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFO4307969.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHQNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            33054..34091
                     /gene="iap"
                     /locus_tag="ACHJWA_001330"
     CDS             33054..34091
                     /gene="iap"
                     /locus_tag="ACHJWA_001330"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFO4307970.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPQSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   34173..34385
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=34234..34263
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaacaca"
     gene            complement(34481..34774)
                     /gene="cas2e"
                     /locus_tag="ACHJWA_001331"
     CDS             complement(34481..34774)
                     /gene="cas2e"
                     /locus_tag="ACHJWA_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFO4307971.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVAFLPVDNQ"
     gene            complement(34771..35694)
                     /gene="cas1e"
                     /locus_tag="ACHJWA_001332"
     CDS             complement(34771..35694)
                     /gene="cas1e"
                     /locus_tag="ACHJWA_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HFO4307972.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPPPARRSVDQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEKVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRGG"
     gene            complement(35691..36341)
                     /gene="cas6e"
                     /locus_tag="ACHJWA_001333"
     CDS             complement(35691..36341)
                     /gene="cas6e"
                     /locus_tag="ACHJWA_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311637.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFO4307973.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGAFRFFVLSQERPAESETFTIECRSFVPELRTGQQLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGQAEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEV"
     gene            complement(36323..37069)
                     /gene="cas5e"
                     /locus_tag="ACHJWA_001334"
     CDS             complement(36323..37069)
                     /gene="cas5e"
                     /locus_tag="ACHJWA_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFO4307974.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSAPD
                     LLSAIISRRDYYTDAWWMVAVATTPDAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNASDVLRNAYQQYQDSFRELKVSLPKLQDECWWEGEHDGLVASKILRRRDV
                     PLNRQQWLFGERTINQGPWLSKEEPCTSQE"
     gene            complement(37080..38135)
                     /gene="cas7e"
                     /locus_tag="ACHJWA_001335"
     CDS             complement(37080..38135)
                     /gene="cas7e"
                     /locus_tag="ACHJWA_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFO4307975.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEEKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRMAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTLRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTEQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(38147..38683)
                     /gene="casB"
                     /locus_tag="ACHJWA_001336"
                     /gene_synonym="cse2"
     CDS             complement(38147..38683)
                     /gene="casB"
                     /locus_tag="ACHJWA_001336"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFO4307976.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAALSANVKAIDERLPFAAQLAAVMSKGRFTRLS
                     AVKTPDELLRQLRRVVRLLNGSVNLDSLAEGVFRWCQESDDLLNHHRRHQRPTEFIRI
                     RWALEYYQAGDVDNEQNQ"
     gene            complement(38680..40242)
                     /gene="casA"
                     /locus_tag="ACHJWA_001337"
                     /gene_synonym="cse1"
     CDS             complement(38680..40242)
                     /gene="casA"
                     /locus_tag="ACHJWA_001337"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HFO4307977.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQSSFAPKDYRRWDDIWEDGLEAEKLREALLSLEHPFQFGPDSPSFM
                     QDFEVLMGDKVQVASLLPEIPGAQTTKFNKDHFIKRGVTEHVCSHCSALALFSLQLNA
                     PSGGKGYRTGLRGGGPMTTLIELQEYQGNQQAPLWRKLWLNVMPQDEADLPLPKKFDD
                     LVFPWLGPTRTSELAGAVVTDDQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQSDA
                     LLSLMTTKNYGANYAMWQHPLTPYRVPLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLNKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDADELLGKWQKEIWLFARQDFDERVFTNPYEPVDLERVMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            complement(40340..43039)
                     /locus_tag="ACHJWA_001338"
     CDS             complement(40340..43039)
                     /locus_tag="ACHJWA_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HFO4307978.1"
                     /translation="MRKYPLSLLKDKNIVTFFDFWGKTRRGEKEGGDGYHLLCWHSLD
                     VAAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLAP
                     ELKIPEGSRKNYEKISHSTLGYWLWNYYLSECEELLPSSSLSSRKLTRVIEMWMSITT
                     GHHGRPPDRIDELDNFLPEDKAAARDFLLEIKALFPLIEIPTFWDDDEGVELLKQLSW
                     YISATVVLADWTGSSTRFFPRVAHPMDIKDYWQKTLVQAQNALTVFPPKAETAPFTGI
                     NTLFPFIEHPTPLQQKVLDLDISQPGPQLFILEDVTGAGKTEAALILAHRLMAARKAQ
                     GLFFGLPTMATANAMYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLV
                     GSEEPDEQTFSQGCAAWFANSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNK
                     ILLADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGQ
                     QEAPFLEKDDYPWLTHVTKSDVNSHRVATRKDVERSVSVGWLHSEQESIARIESAVSQ
                     GKCIAWIRNSVDDAIKVHRQLLARGVIPASSLSLFHSRFAFSDRQRIEMETLARFGKE
                     DGSQRAGKVLICTQVLEQSVDCDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRD
                     GKDERSPPELLILAPVWDDAPGDEWFGSAMRNSAYVYPDHGRIWLTQRVLREQGAIQM
                     PHAARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFKPGYAANINDY
                     LPEKLSTRLAEESVSLWLATCIAGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLL
                     EGEAFRQWCIEQRQDPEMANVILVTDDESCGYSAREGLIGKVD"
     gene            complement(43231..43383)
                     /locus_tag="ACHJWA_001339"
     CDS             complement(43231..43383)
                     /locus_tag="ACHJWA_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO4307979.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(43647..44381)
                     /gene="cysH"
                     /locus_tag="ACHJWA_001340"
     CDS             complement(43647..44381)
                     /gene="cysH"
                     /locus_tag="ACHJWA_001340"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO4307980.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAQLEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKNGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            complement(44455..46167)
                     /gene="cysI"
                     /locus_tag="ACHJWA_001341"
     CDS             complement(44455..46167)
                     /gene="cysI"
                     /locus_tag="ACHJWA_001341"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFO4307981.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(46167..47966)
                     /gene="cysJ"
                     /locus_tag="ACHJWA_001342"
     CDS             complement(46167..47966)
                     /gene="cysJ"
                     /locus_tag="ACHJWA_001342"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFO4307982.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLTAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGVVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQEEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            48285..48647
                     /gene="queD"
                     /locus_tag="ACHJWA_001343"
     CDS             48285..48647
                     /gene="queD"
                     /locus_tag="ACHJWA_001343"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFO4307983.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            48725..49996
                     /gene="ygcN"
                     /locus_tag="ACHJWA_001344"
     CDS             48725..49996
                     /gene="ygcN"
                     /locus_tag="ACHJWA_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO4307984.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDCATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLARFKTHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDTALTGAQAAAQTLISACQHRE
                     PQNLFPLYHHNVERSLLWDVLQRYQHVPVLLQRPGWYRTWPALMQDISRDLWDQGDKP
                     VPPLRQLLWRHLRRHGLWNLAGDVIRSVRCL"
     gene            49987..50247
                     /gene="ygcO"
                     /locus_tag="ACHJWA_001345"
     CDS             49987..50247
                     /gene="ygcO"
                     /locus_tag="ACHJWA_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO4307985.1"
                     /translation="MSVARNLWRAADAPHIVPADSVERQMVQRLINACPVGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            50264..50839
                     /gene="ygcP"
                     /locus_tag="ACHJWA_001346"
     CDS             50264..50839
                     /gene="ygcP"
                     /locus_tag="ACHJWA_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFO4307986.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     QIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(50987..51847)
                     /gene="ygcQ"
                     /locus_tag="ACHJWA_001347"
                     /pseudo
     CDS             complement(50987..51847)
                     /gene="ygcQ"
                     /locus_tag="ACHJWA_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
     gene            complement(51844..52623)
                     /gene="ygcR"
                     /locus_tag="ACHJWA_001348"
     CDS             complement(51844..52623)
                     /gene="ygcR"
                     /locus_tag="ACHJWA_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFO4307987.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAHGNSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFASE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDAPFI
                     TLEQRTEHGLRCCRIRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAELPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(52601..53937)
                     /gene="ygcS"
                     /locus_tag="ACHJWA_001349"
                     /pseudo
     CDS             complement(52601..53937)
                     /gene="ygcS"
                     /locus_tag="ACHJWA_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417251.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(54031..55485)
                     /gene="ygcU"
                     /locus_tag="ACHJWA_001350"
     CDS             complement(54031..55485)
                     /gene="ygcU"
                     /locus_tag="ACHJWA_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFO4307988.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVNCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(55555..56340)
                     /gene="ygcW"
                     /locus_tag="ACHJWA_001351"
     CDS             complement(55555..56340)
                     /gene="ygcW"
                     /locus_tag="ACHJWA_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO4307989.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     VFIPSFVKDNGETKEMIENQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASQASNYVNGHLLVVDGGYLVR"
     gene            56659..57936
                     /gene="yqcE"
                     /locus_tag="ACHJWA_001352"
     CDS             56659..57936
                     /gene="yqcE"
                     /locus_tag="ACHJWA_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFO4307990.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTVGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSTSLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSVLGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTQPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            57963..59441
                     /gene="ygcE"
                     /locus_tag="ACHJWA_001353"
     CDS             57963..59441
                     /gene="ygcE"
                     /locus_tag="ACHJWA_001353"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFO4307991.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSED
                     AAVMEKFNIPRHMLFDVQMPGTVLGQITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASCVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKALTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
BASE COUNT        14189 a        16205 c        15307 g        14072 t
ORIGIN      
        1 ttggtatctg gatcggtagc gccatttcgg cgctggttta caccattcat ggttatctgg
       61 gctggctttc agtcgccatt atgggcgcgc tgtggcctct gctggtaatg accgggatgc
      121 accgcgtctt tacgctaacc atcattcaga ccattgccga aaccggcaaa gaagggatgg
      181 tcatgccgtc agagatcggc gctaacctgt cgctgggcgg ttcatcactg gcggtggcgt
      241 ggaaaacgaa aaacccggaa ctgcgccaga ctgcgctggc tgcggcggca tcagccatta
      301 tggcggggat ttccgaaccg gcgttatacg gcgtggcgat ccgcctgaaa cgtccgctta
      361 tcgccagtct tatcagcggt tttatttgcg gcgcggttgc cggtatggcg gggcttgcca
      421 gccactcaat ggcagcgccg gggctattta ccagcgtgca gttcttcgat ccggcgaatc
      481 caatgagcat cgtctgggtg ttcgcggtca tggcgctggc ggtggtgctg tcgtttatcc
      541 tcacactgtt gctcggcttt gaggatattc ctgttgagga agcggctgcc gaggcgcgaa
      601 agcatcagag cgcacaaccg accgtcgcca aagaagtaag tcttaattga ggatgaaaat
      661 gtcagtattt ccagaaggtt ttttatgggg cggcgcgctt gccgccaacc agtctgaagg
      721 tgcgttccgt gaaggtggca aaggactgac aacggtcgat atgatcccac acggcgagca
      781 tcgaatggcg gtgaaactgg ggctggaaaa acgttttcag ttgcgcgatg acgagtttta
      841 tcccagccat gaggcgacgg gtttttatca tcgttataaa gaagatatcg ccctgatggc
      901 agagatggga ttcaaagtgt tccgtacctc aattgcctgg agccgcccct tcccgcaggg
      961 tgatgaactg acgcccaacc agcagggcat tgctttttac cgttctgtct ttgaagagtg
     1021 taaaaagtac ggtatcgaac cgctggtcac attgtgccac ttcgatgtgc cgatgcagct
     1081 ggtcaccgaa tatggctcct ggcgtaaccg caagctggtg gagtttttca gccgctacgc
     1141 caggacctgc tttgaagcat ttgatggtct ggtgaaatac tggctaacct tcaatgaaat
     1201 caacattatg ttgcatagcc cgttctccgg cgcggggctg gtgtttgaag aaggtgaaaa
     1261 tcaggatcag gtgaaatatc aggccgcgca tcaccagctg gttgccagtg cgctagccac
     1321 caaaatcgcc catgaggtta acccgcaaaa tcaggtgggc tgtatgctgg cgggcggtaa
     1381 cttctaccct tacagttgca agccggaaga tgtctgggcg gcgctggaga aagatcggga
     1441 aaacctgttt tttatcgatg tgcaggcgcg gggcgcgtat ccggcttact ctgctcgcgt
     1501 attccgcgaa aaaggggtaa ccatcgacaa agcaccgggc gatgatgaga tcctgaaaaa
     1561 caccgtcgat tttgtctctt tcagctatta cgcctcgcgc tgcgcctcgg cggagatgaa
     1621 caccaacaat agcagtgcgg cgaacgtagt gaaatcgctg cgtaacccgt atctacaggt
     1681 gagcgactgg ggctggggaa ttgatccact cggtctgcgt atcaccatga acatgatgta
     1741 cgaccgctat cagaagccgc tgtttctggt ggaaaacggc ctcggcgcaa aagatgaact
     1801 tgctgccaat ggcgagatta acgatgacta tcgcatcagc tatttacgcg aacatatccg
     1861 cgcaatgggc gaagcgattg cagatggcat tccgctgatg ggctacacca catggggctg
     1921 tattgattta gtttccgcct ctacgggtga aatgagcaaa cgctacggtt ttgtctttgt
     1981 tgaccgtgac gacgcaggca acggcacgct gacgcgcaca cgtaagaaat cgttctggtg
     2041 gtataaaaaa gtgattgcca gtaatgggga agatttagag taggtaacct actgtcgcac
     2101 taaccaggaa ggcccgataa gcgttgcgca tcgggcaatc tggggtttgc tatcagttct
     2161 gattccctca tcaagaacat ccctgtcctg attacttatt gaaaaactac tcttcttcca
     2221 ccgctaactg cgcgaagccg ccgttccctt cccagccttc cagtcgttga taaacggttt
     2281 ctaccgcatc tttaatcggc tgggtcatcg ggtagtaaaa gccgacgata tccggctgga
     2341 tgccgaggaa aatcacttcg ccaatatctt ctttcaactg atcgataagg tagttcaacg
     2401 gcatgttatg ggtagtcatc ataaacatct cggcgatatc atccgggtcg atgatgcgga
     2461 tttcgccggg gtttagcccc atatccgtgg cgtcgacaat cagcagccgt gtcgggcgca
     2521 gttcgcggat agcgacaatg tcgttttccg gtgcgctacc gccgtcaatc accacccagt
     2581 tacctttcgg cgcggcggcg cacttttccg ccagcagcgg acctgcgcca tcatcaccca
     2641 tcatgctatt gccaacacag agtaaaacgt cagtcacgca gtctcctcac catcaaatag
     2701 atggcgtttt cctgatgaat atcatgcagc atacttaaca gcattgtact ccattcctgc
     2761 tgctgcgaag tttgcgctgc ccgggcttta tcgaatgcat tggccagcat cggcacatgg
     2821 ttaatgtcga tgacgatctc accatatttc ggcacacctt ccattttgcg gcgggcttcg
     2881 ctgcccgcct ccagagtggc aatccatgcc agatattcat cccacgggca ggtgagcgcc
     2941 gcttccaggc aatcgataac cccaaggtgg tgaccaatcg ccaggctgta atagaccacc
     3001 tgctgcgcct cggcgggcgt ggcatcgttc tcatcaataa atttacggct cagttgactg
     3061 aacaccacct tttcactcat cggatacgcg cctcttcaac aacatgattc agatggctga
     3121 caatctcgtt cagacgcgga tcgttttccg cttccagcca gcgtgcaacc tgttcttcgc
     3181 cctgccctaa ctgtgtaagg taatcatcgg caatctgacg accgtaacga taacccgcca
     3241 gtcgacgtgc ttcgcgatcc actttcacac gcagcggctg caccatatcg ccatgcagga
     3301 tctccgccgg ttgttcatcc tgctcacccg gcccacgggc gtgaattttc tgctccagca
     3361 ggccgagcgc cattgcaaag ccgtacagcg tggcggctgg cgttggcggg cagccaggga
     3421 tataaacatc caccgggacg attttatccg taccgcccca cacgcagtag agatcgtgga
     3481 agatcccgcc gctgttaccg caggcaccgt aggagataca aattttcggg tccggcgcgg
     3541 actgccacgc acgcagcgca ggggatcgca ttgcacgggt gaccgcgccg gtaaacagta
     3601 aaatatccgc atgacgcggt gaaggaacga ctttaatgcc gaagcgttct gcatcgaaca
     3661 gcggcgacaa agtggcgaaa atttcgattt cgcagccgtt gcagccgccg cagtccacgc
     3721 ggtaaacgta ggcagaacgt ttgatttttt tcagtaacga cgccttcatg ctggcgatgg
     3781 attcatccac cgtcatcggg accggaatgc cgttggcgtc acgggggcct aataaattgc
     3841 tcatcagatg gcctctttca tatggcgagt cagttcaata cggtcggacg gcaccaggca
     3901 tttctggcgc ttacattccg ggcaagtctc aaagctttcg cggtggtttt ccgcgcggct
     3961 gtcgccattg tgcttaagca gcgcaatggc gtagtcgatc tctttctgga cggcgaaagg
     4021 acgattgcag acgcggcagt tgcacagcgc gaagcgggac tgttgcagga agtcttcttt
     4081 cttccacacc gccagttcgt actcttgcga cagtttgatt gccgccgtcg ggcagacttc
     4141 ttcgcagcgt ccgcagaaga tgcagcgccc gagattgaac tcccacgcca gctcaccggt
     4201 ggcgaggtca gtttcaaccg ttaaggcgtt tgacgggcag gcattgacgc aggccgcgca
     4261 gccgatgcac tgctgcgggt tctgctctgg cttaccacgg aagtttttat caaccgcaat
     4321 cggttccagc ggataagacg aggtcgccgt gccggttttg atgacttttt tgataaaggt
     4381 aaacatggcg attccttatt tcagcggcga gtttttacgc tcaatgctgt aacgctcgag
     4441 ttctttgtac ggcaccactt tgctcttttt cttacgcaca tcgaccacgg tcatgcggtc
     4501 ggtacaggag tagcaagggt ccagactacc gataatcagc ggcgcatcag aaacggtgtt
     4561 gccgcgcagc atgtagcgca gggtcggcca gttcgcgtag gtcgcggcac ggcagcgcca
     4621 gcggtacagc ttctggttgt cgccggtcat gctccagtgg atatcatcgc cgcgcggcgc
     4681 ttcggcaaag cccagcgcaa aacggtgcgg aatgtaggta aagccttcca ccatcagcgg
     4741 gccgcccggc aggttatcca gaccgtagtc gatcatgttc agcgcggtat agacttcgtt
     4801 aatacgcact ttcagacgcg aaataacgtc gcagccctgc tcgctgtgga cttccattgg
     4861 cagcaggcca taaccaacaa acgggtgatc ggcgcgggta tcgcgggcgt gaccgctggc
     4921 acggaccatc gggccgacgt tactgaagtc gcgagcgatt tccgggtcca gacgaccaat
     4981 gccgacagtg cgctgttcca tgttcggcgt actcagcagc acatccacca gctcctgcac
     5041 ttcacgacgc atctgttgcg ccagttgacg ggtctggatc atgtcatctt tcagcagatc
     5101 gcgacgaatc ccgccgatca ggttcaggcc gtaggtttta cgcgcaccgg taaggatctc
     5161 tgccattttc atggaggttt cacgcacgcg gaagaactgc ataaagccgg agtcaaagcc
     5221 ggtaaagtga caggcgaggc cgaggttgag cagatgcgag tgcagacgtt ctacctccag
     5281 cagaatggcg cggatcatct gcgcacgttc tggcacctga atacccatcg cgttttccac
     5341 cgacgtggtg taggcggtgc tgtgagcaaa accgcagatc ccgcacacac ggtcagagag
     5401 gaaggtcact tcgttataac ccatgcgggt ttctgccagt ttttccatgc cgcgatggac
     5461 atagaacagg cggtagtcgg cgtcgataat gttttcgcca tcgacgaaca gacggaagtg
     5521 gcccggttcg tcagaagtga cgtgcagcgg accaatcggc acgacgttgt ttttcttgtc
     5581 gcccagttcg ttgatgaact cgtaggtttc agcatcggtg gtcggtgccg gacgctgacg
     5641 ataatccatg ctgtctttac gcagcggata aagttcatcc ggccagtcat ccggcagcac
     5701 cagacgacgt tcatccggca aaccaaccgg aatcaaaccg tacatatcac gtacttcacg
     5761 ctcgccccac accgcagccg gaacgcgcgg cgtcacggaa ggatactccg gtttgttggc
     5821 atcaacttcg acgcgaaccg ttacccagca cttagtcccc ttctccatcg acagcacgta
     5881 gtaaacggcg taatgaccat tcagtttgcg ttcgtcgtta ccaaaaagca ccgacagcca
     5941 gcccccctgc ttgtagtaga gaaactccac cacttccggc agatagttca cctttacggt
     6001 gacagtcagc tgatctttgg tctgccaggc gtggtccagc acgacgcccg gaaatgcctc
     6061 attcagcgcg gcgagataat gttgacctaa tttttcttca gacatgctca aactctcttt
     6121 aatcacgccg ccagcaagga gacgaacgct aaaaatgcaa agccaaaccc agcccaggta
     6181 atgcgcggag taatatcaag acgcagacgg gccatgctgt tttcgaacag cgcgataacc
     6241 aggacgccga ccaccagttt gacgatggca atcaccaacg ccagcagcag cccaccgacg
     6301 gtgaaggttt ccatttgtcc ccacggaata aacaccccga cgaacatctg cagcaccacc
     6361 aactgtttca ggctgatacc ccatttcatg acgccaaagc cgctgccgct gtactcagag
     6421 agcggacctt cctgcaactc ctgctcggct tccgccaggt cgaacggcag tttgcccatt
     6481 tcgataaaag tggcgaacgc acaggcacaa agcgccagca ccagcgggat gctctggctc
     6541 agcggccagt gataaacggt gtcggtgatg ttgctgatgt tggtggaact ggcaacctgt
     6601 gcggcaaccc acagaccaag cagcagcatc ggttcgacca gcacgccaag catcgcttca
     6661 cggctcgcgc cgatagcggt aaacggacta ccggtatcca gaccagaaat ggcaaagaag
     6721 aaacgcgcga tggcaaagag atacagtaag gtgatcaaat cacccagttg cggcagcgga
     6781 gaaccgacgg tcaccaccgg cagcgcagta gcgatagtca gcatgacgcc caccatcaca
     6841 tacggcgtca ggcggaacac ccagccggag gcatccgggc caacgctctg gcgacccagc
     6901 agtttgataa tgtcgcgata ctcctgcaac acgcccggcc cgcgacggtt atgcaagcgg
     6961 gcgcgcgcca cgcgggtaat accggagagc agcggcgcaa cggcaaatag caccagcgcc
     7021 tgaattaacg gatataaaac actcattctc aggctcctcg tgaaacaata atcaccacca
     7081 gcaccgccag ttcaaccagc gccatccggc ggaacagcaa cgcactgcct tcgcactgcc
     7141 agcccggcac cagcgacacc ggattcagcc atttacgcag tttcagcacc ggcgcaaacg
     7201 cctgtttcac cggcatggca aaaccgtggg cggtaattac cattgatttt tcatggtcgt
     7261 agccgcacac ccaggccgcg ccgcgggaac gcgatggcaa acgatcgcct ttgcaaatcg
     7321 ccataatgat gaatggcagc agcgggcagg caatcagcag caacgtgatc atcggttgag
     7381 aaacggtggt gttagcaggt tccagcggca gaggtacagc agcagagagc atcggcagca
     7441 gccacggcgc agcaacaccg ccaattacgc agcaaatcgc cagtgccact acgcttacgc
     7501 tcatcaggag cggtgcacag gtggcgtttt cggcttcttt ggtacgcggc gcgccgagga
     7561 acgtgacgcc atagactttc gccatacaca tcaccgccag cgcaccggta attgccagac
     7621 ccacagcgag cagcggcccc agcagacggg caacaaacgc gccactattg ctcagtttga
     7681 agaaggattg atagataacc cattccccgg caaaaccgtt cagcggcggc agtgcagcca
     7741 ttgccatcag cccgactaac atagcgatgg agataaccgg cattttcttg ccaataccac
     7801 cgagtttttc gatatcgcga tgaccggtac ggaaccagac gctccccgcc ccgaggaaca
     7861 gtacgctttt gaacaggcta tggttaagca gatggtacag accaccgacc aggccaagag
     7921 caatcagcgc cggttgttcg agcgcgatac ccgttacgcc agcgcccagc cccagcagga
     7981 taatgccgat attttccagg gtgtggtaag ccagtagacg ctggatatta tgttccatca
     8041 acgcatacag accgccgaca aacgcggtga tcataccgag caccagcagc gcgatccccc
     8101 accacagcgg tgcattaccg cccagcagtg acagggttaa aatgcccagc aggccaattt
     8161 tcatgactac cgtagaaaac aacgcggcag ctggcgcaga ggcgttcgca tgtgcctgcg
     8221 gcacccagcc gtgcagcgga ataatcccgg ccagcaggcc aaagccaatc acactgagca
     8281 gccagatatc ggaaccgagc ggcagctgtt gcatacgcat atccagcagg cgcagatcca
     8341 gcgtgccgta acgctgccac agcagccagc aagcaatcgc cagcagcaga gtgccaagac
     8401 gccccagcgc aaaccacagt ttgccctctt tgctgttgct ggtgaggaac accgcacaca
     8461 aggccatgac ttcggccatt accacgaaca tgccgaggtt gctggcaatg acggcgcaga
     8521 cggcggcagc catcaacata ttgatctgca agccgttgca cttcacctgc gcgtggcgat
     8581 gccagtcaat gttgtagagg ctgacaaaca gaccgcacag accgagcgta atcagccaaa
     8641 tcgcgttaag cggagagatt tgcacatcgt agcttaccag cgacagcgca ccgctcacgc
     8701 caaccgcgcc agtcagtaca gtgaagcccg cggctgccgt atacagacta ccaaccgcgc
     8761 cgccaattcc ggctatccag ccgcttagcg ccttttgaaa agaaaagaga aatgccagaa
     8821 cagcggcggc gacaaaccac gccacgccgc tattgatcag ggaaattgcg ctcatttagc
     8881 ctctccactt tgagcctgct gaaacaaggt gagatcgcca aagtccgtgt taaaggtcag
     8941 ctcacgctta cgtttgctga cgcgggcgat atcggtgtta tccaccagat gcagggcttt
     9001 agtcgggcac atccgcacgc aggccggacc ttgttcatca aagctacaaa ggtcacattt
     9061 gacggcgatc gcgcgaatac ccggcaccca gtcaagcagc gtactgatac gcgccggagc
     9121 aggcggtgcc ggtggcgctt tcggggtatt ggcatttgca ggaatatcca gcggacggct
     9181 gccggaaaat tcaattgcgc caaacgggca ggcgatgccg cacagcttgc agcttacgca
     9241 caggctttca ttcaactgca cggccccatc aacgcgggtg atggcgttaa ccgggcagac
     9301 caccgcgcag ggtgcatctt cacagtggtg acagagctgc ggcgcagatt ctttttcatt
     9361 tagcatcact ctcaggcgcg gcattgattg caggccgtgc tggcgatgcg tctctgaaca
     9421 ggcggcctca caagtgtggc aaccgataca gagcgtggag tcagcaatta caaaacgatt
     9481 caccaggcat tcctcaggtg attgtcattt ttgacgaaaa catgccgatg aaatgtcatt
     9541 ttcgacactc atcgacacgc ccatccccaa acaggcgtaa cgcctgcaaa acgggcaaag
     9601 cctcagctca tgctgccggg ctttgtccct ttaccagttg gcttaaattc accggcacat
     9661 tgttttcaat agcggtgtat aagctgtcgt aaacgccagc gattttttcg agatagtgtt
     9721 ccagaacctg ttcgcgatcg cggatgttga ccttgccatc aatcatgccg tcaatgctca
     9781 gttgcgcctg cgcaatccgc tgtttgtctt cgccatcttt tgtttcgacg taatactcga
     9841 tggtgtggct gttaaagcca tccgccaggg tgacgtaaat acgaaaatgt tcaaaaagga
     9901 cgaaacagag ttctttgtcc ggcgcgcagc tcatggcgtg atgcaggcgc gctttcgata
     9961 acgtgatccc ctgaaccagc gaattgcagt agatgtgcca ctggtcctgt aggcgacgat
    10021 gccgctgtgc gatgtaatcg gctttctcgc ttatttccca aatagtcatt gtcaggttac
    10081 ccgtttaaca gagatgccag ctttaagcat tttttgtgcc aacttttaat ttattattat
    10141 taaagcgatt tttaagattt tggtgaattt cgtcgccgtg tcgacgtgtc atttcgacat
    10201 catcgacatt attcactgca gggataatca acactggcac aattattgct tgtagctggc
    10261 aatagttaat gggaggcgat atgcacgaaa taaccctctg ccaacgggca ctggaattga
    10321 tcgaacagca ggccgcaaaa cacggcgcaa aacgcgtaac tggggtctgg ctcaaaattg
    10381 gcgcattttc ttgtgtcgaa accagctctc ttgccttttg ttttgatctg gtttgccgcg
    10441 gcagcgtggc ggaaggttgt aaactgcacc tcgaagaaca agaggccgaa tgctggtgtg
    10501 aaacatgcca acagtatgtg acgctactga cccagcgcgt ccgccgctgt ccacagtgtc
    10561 atggtgacat gctacagatt gtggcagacg acggtttaca gattcggcgg atagaaatag
    10621 accaggagtg agcgatgtgt acaacatgcg gttgcggtga aggcaacctg tatatcgagg
    10681 gtgatgaaca taaccctcat tccgcgtttc gtagcgcgcc atttgccccg gcggcacgcc
    10741 cgaagatgaa aatcaccggc attaaagcgc ctgaatttac ccccagccag actgaagaag
    10801 gcgacctgca ttacggtcat ggcgaagcgg gcactcacgc accgggcatg agccagcgtc
    10861 ggatgctgga agtcgaaatt gacgtgctgg acaaaaataa ccgtctggct gaacgcaacc
    10921 gcgcgcgctt tgctgcctgc aagcaactgg tgctcaacct ggtttccagc ccaggttccg
    10981 gtaaaaccac cctgctgacg gaaaccttaa tgcgcctgaa agacagcgtt ccgtgcgcgg
    11041 ttattgaagg tgaccagcaa accgtgaacg atgccgcacg cattcgcgct accggcacac
    11101 cagcgattca ggtgaacacc ggtaaaggct gccatcttga cgcacagatg attgccgacg
    11161 ccgcaccgcg tctgccactg gacgataacg gtattctgtt tatcgaaaac gttggcaacc
    11221 tcgtatgccc ggccagcttc gatctcggtg aaaaacacaa agtggcggtg ctttccgtta
    11281 ccgaaggtga agacaaaccg ctgaaatatc cgcatatgtt tgccgccgcc tcgctgatgc
    11341 tgctcaacaa agttgacctg ttgccgtatc tcaactttga cgttgagaag tgcatcgcct
    11401 gcgcccgcga agtcaatcca gaaattgaaa tcatccttat ttccgccacc agcggcgaag
    11461 ggatggacca gtggctgaac tggctggaga cacagcgatg tgcataggcg ttcccggcca
    11521 gatccgcacc attgacggta accaggcgaa agtcgacgtc tgcggcattc agcgcgatgt
    11581 cgatttaacg ttagtcggca gctgcgatga aaacggtcag ccgcgcgtgg gccagtgggt
    11641 actggtacac gttggctttg ccatgagcgt aattaatgaa gccgaagcac gcgacactct
    11701 cgacgccttg caaaacatgt ttgacgttga gccggatgtc ggcgcgctgt tgtatggcga
    11761 ggaaaaataa tgcgttttgt tgatgaatat cgcgcgccgg aacaggtgat gcagttaatt
    11821 gagcatctgc gcgaacgtgc ttcacatctc tcttacaccg ccgaacgccc tctgcggatt
    11881 atggaagtgt gcggtggtca tacccacgcc atttttaaat tcggcctcga ccagttactg
    11941 cctgaaaacg ttgagtttat ccacggtccg ggttgcccgg tgtgcgtact gccgatgggc
    12001 agaatcgaca cctgcgtgga gattgccagc catccggaag tcatcttctg tacctttggc
    12061 gacgccatgc gcgtgccggg gaaacaggga tcgctgttgc aggcaaaagc acgcggtgcc
    12121 gatgtgcgca tcgtctattc gccgatggat gcgttgaaac tggcgcagga gaatccaacc
    12181 cgcaaagtgg tgttcttcgg cttaggtttt gaaaccacca tgccgaccac cgccatcact
    12241 ctgcaacagg cgaaagcgcg tgatgtgcag aatttttact tcttctgcca gcatattacg
    12301 cttatcccga cgctgcgcag tttgctggaa cagccggata acggtatcga cgcgttcctc
    12361 gcgccgggcc acgttagtat ggttatcggc actgatgcct ataattttat cgccagcgat
    12421 tttcagcgtc cgctggtggt ggctggtttc gaaccccttg atctactgca aggcgtggtc
    12481 atgctggtgg agcagaaaat agcggcccac agcaaggtag agaatcagta tcgtcgggtg
    12541 gtaccggatg ccggtaacct gctggcgcaa caggcgattg ccgatgtgtt ctgtgtcaac
    12601 ggcgacagcg aatggcgcgg cttaggcgtg attgaatctt ctggcgtgca cctgacgccg
    12661 gattatcaac gattcgatgc cgaagcacat ttccgcccgg caccgcagca ggtctgcgat
    12721 gacccgcgcg cgcgttgtgg cgaagtcttg acgggcaaat gtaagccgca tcaatgcccg
    12781 ctgtttggta acacctgtaa tcctcaaacc gcgtttggtg cgctgatggt ttcctccgaa
    12841 ggagcgtgcg ccgcgtggta tcagtatcgt cagcaggaga gtgaagcgtg aataatatcc
    12901 aactcgccca cggtagcggc ggccaggcga tgcagcaatt aatcaacagc ctgtttatgg
    12961 aagcctttgc caacccgtgg ctggcagagc aggaagatca ggcacgtctt gatctggcgc
    13021 agctggtagc ggaaggcgac cgtctggcgt tctccaccga cagttacgtt attgacccgc
    13081 tgttcttccc tggcggtaat atcggcaagc tggcgatttg cggcaccgcg aatgacgttg
    13141 cggtcagtgg cgctattccg cgctatctct cctgtggctt tatcctcgaa gaaggattgc
    13201 cgatggagac actgaaagcc gtagtgacca gcatggcaga aaccgcccgc acggcaggca
    13261 ttgccatcgt tactggcgat actaaagtgg tgcagcgcgg cgcggcagat aaactgttta
    13321 tcaacaccgc gggcatgggc gcaattccga cgaatattca ctggggcgca cagacgctaa
    13381 ccgcaggcga tatattgctg gttagcggta cactcggcga ccacggggcg actatcctta
    13441 acctgcgtga gcagctgggg ctggatggcg aactggtcag cgactgcgcg gtgctgacgc
    13501 cgcttattca gacgctgcgt gacattcccg gcgtgaaagc gctgcgtgat gccacccgtg
    13561 gtggtgtaaa cgcggtggtt catgagttcg cggcagcctg cggttgcggt attgaaattt
    13621 ctgaatcagc gctgccggtt aaacctgccg tgcgcggcgt ttgcgaattg ctgggactgg
    13681 acgccctgaa ctttgccaac gaaggcaaac tggtgatcgc cgttgaacgc aacgcggcag
    13741 agcaagtgct ggcagcgtta cattcccatc cactggggaa agacgcggcg ctgattggtg
    13801 aagtggtgga acgtaaaggt gttcgtcttg ccggtctgta tggcgtgaaa cgaaccctcg
    13861 atttaccaca cgccgaaccg cttccgcgta tatgctaata aaattctaaa tctcctatag
    13921 ttagtcaatg accttttgca ccgctttgcg gtgctttcct ggaagaacaa aatgtcatat
    13981 acaccgatga gtgatctcgg acagcaaggg ttgttcgaca tcactcggac actattgcag
    14041 cagcccgatc tggcctcgct gtgtgaggct ctttcgcaac tggtaaagcg ttctgcgctc
    14101 gccgacaacg cggctattgt gttgtggcaa gcgcagactc aacgtgcgtc ttattacgca
    14161 tcgcgtgaaa aagacacccc cattaaatat gaagacgaaa ctgttctggc acacggtccg
    14221 gtacgcagca ttttgtcgcg ccctgatacg ttgcattgca gttacgaaga attttgtgaa
    14281 acctggccgc agctggccgc aggtgggcta tacccaaaat ttggtcacta ttgcctgatg
    14341 ccactggcgg cggaagggca tatttttggt ggctgtgaat ttattcgtta tgacgatcgc
    14401 ccctggagcg aaaaagagtt caatcgtctg caaacattta cgcagatcgt ttctgtcgtc
    14461 accgaacaaa tccagagtcg cgtcgttaac aatgtcgact atgagttgtt atgccgggaa
    14521 cgcgataact tccgcatcct ggtcgccatc accaacgcgg tgctttcccg cctggatatg
    14581 gacgaactgg tcagcgaagt cgccaaagaa atccattact atttcgatat tgacgatatc
    14641 agtatcgtct tacgcagcca ccgtaaaaac aaactcaaca tctactccac tcactatctt
    14701 gataaacagc atcccgccca cgaacagagc gaagtcgatg aagccggaac cctcaccgaa
    14761 cgcgtgttca aaagtaaaga gatgctgctg attaatctcc acgagcggga tgatttagcc
    14821 ccctatgaac gcatgttgtt cgatacctgg ggcaaccaga ttcaaacctt gtgcctgtta
    14881 ccgctgatgt ctggcgacac catgctgggc gtgctgaaac tggcgcaatg tgaagagaaa
    14941 gtgtttacca ctaccaatct gaatttactg cgccagattg ccgaacgtgt ggcaatcgct
    15001 gtcgataacg ccctcgccta tcaggaaatc catcgtctga aagaacggct ggttgatgaa
    15061 aacctcgccc tgaccgagca gctcaacaat gttgatagtg aatttggcga gattattggc
    15121 cgcagcgaag ccatgtacag cgtgcttaaa caagttgaaa tggtggcgca aagtgacagt
    15181 accgtgctga tcctcggtga aactggcacg ggtaaagagc tgattgcccg tgctatccat
    15241 aatctcagtg ggcgtaataa tcgccgcatg gtcaaaatga actgcgcggc gatgcctgcc
    15301 ggattgctgg agagcgatct gtttggtcat gagcgtgggg cttttaccgg tgccagcgcc
    15361 cagcgtatcg gtcgttttga actggcggat aaaagctccc tgttcctcga cgaagtgggc
    15421 gatatgccac tggagttaca gccgaagttg ctgcgtgtat tgcaggaaca ggagtttgaa
    15481 cgcctcggca gcaacaaaat cattcagacg gacgtgcgtc taatcgccgc gactaaccgc
    15541 gatctgaaaa aaatggtcgc cgaccgtgag ttccgtagcg atctctatta ccgcctgaac
    15601 gtattcccga ttcacctgcc gccactacgc gagcgtccgg aagatattcc gctgctggcg
    15661 aaagccttta ccttcaaaat tgcccgtcgt ctggggcgca atatcgacag cattcctgcc
    15721 gagacgttgc gcaccttgag caatatggag tggccgggta acgtacgcga actggaaaac
    15781 gtcattgagc gcgcggtatt gctaacacgc ggcaacgtgc tgcagctgtc attgccagat
    15841 attgctttac cggagcctga aacgccgcct gccgcaacgg ttgtcgctca ggagggcgaa
    15901 gatgaatatc agttgattgt tcgcgtgctg aaagaaacta acggcgtggt tgccgggcct
    15961 aaaggcgctg cgcaacgtct ggggctgaaa cgcacgaccc tgctgtcacg gatgaagcga
    16021 ctgggaattg ataaatcggc attgatttaa ctgcaaattg ccggacagat ctgcctgtcc
    16081 ggcatactat tcacgaggtt ttttcggacg atatttttcc ggcagttctg gcaccggaca
    16141 cttgtcatcg atgagatgac gcacggttaa gatcggatga cgccacagca ttctcggccc
    16201 ggcccaacgc ataatctgtt tcatctcttc acgctttgca ggctggtaac agtgcaccgg
    16261 acactgctta caggctggtt tctcttcgcc gaacacacat ttatccagcc gcttttgcgc
    16321 gtaaacaaac aacgcctcgt aatgctccgg ctccgctgac gcctgcgggc atttcgcttg
    16381 ataaagatcg atcatttttt taatcgtcag tttttcacga gagatacgct tgccggacat
    16441 actgcctcca cctcattaag atgcatttat attacaactt aatcttaaag ggcactatga
    16501 ctctaaagaa gaagggttag ccaaccgata caattttgcg tacttgcttc ataagcatca
    16561 cgcaaaagct gcaaaacagc atctttcccg gaaccagcat caagaactcg ccgttcgctt
    16621 cttcccctga aatgattaac tccggtatca tgtgcgcctt atgtgattac aacgaaaata
    16681 aaaaccatca cacctcattt aatatcaggg aaccggacat aaccccatga gtgcaataga
    16741 aaatttcgac gcccatacgc ccatgatgca gcagtatctc aagctgaaag cccagcatcc
    16801 cgagatcctg ctgttttacc ggatgggtga tttttatgaa ctgttttatg acgacgcaaa
    16861 acgcgcgtcg caactgctgg atatttcact gaccaaacgc ggtgcttcgg cgggagagcc
    16921 gatcccgatg gcggggattc cctaccatgc ggtggaaaac tacctcgcca aactggtgaa
    16981 tcagggcgag tccgttgcca tctgcgaaca aattggcgat ccggcgacca gcaaaggtcc
    17041 ggttgagcgc aaagttgtgc gtatcgttac gccaggcacc atcagcgatg aagccctgtt
    17101 gcaggagcgt caggacaacc tgctggcggc tatctggcag gacagcaaag gtttcggcta
    17161 cgcgacgctg gatatcagtt ccggtcgttt tcgcctgagc gaaccggctg accgggaaac
    17221 gatggcggca gaactgcaac gcactaatcc tgcggaactg ctgtatgcag aagattttgc
    17281 tgaaatgtcg ttaattgaag gccgtcgcgg cctgcgccgt cgcccgctgt gggagtttga
    17341 aatcgacacc gcgcgccagc agttgaatct gcaatttggg acccgcgatc tggtcggttt
    17401 tggcgtcgag aacgcgccgc gcggactttg tgctgccggt tgtctgttgc agtatgcgaa
    17461 agatacccaa cgtacgactc tgccgcatat tcgttccatc accatggaac gtgagcagga
    17521 cagcatcatt atggatgccg cgacgcgtcg taatctggaa atcacccaga acctggcggg
    17581 tggtgcggaa aatacgctgg cttctgtgct cgactgcacc gtcacgccga tgggcagccg
    17641 tatgctgaaa cgctggctgc atatgccagt gcgcgatacc cgcgtgttgc ttgagcgcca
    17701 gcaaactatt ggcgcattgc aggatttcac cgccgagttg cagccggtac tacgtcaggt
    17761 cggcgacctg gaacgtattc tggcgcgtct ggcgttgcgt accgctcgcc cacgcgatct
    17821 ggcccgtatg cgtcacgctt tccagcaact gccggagctg cgtgcgcagt tagaaactgt
    17881 tgatagtgca ccagtacagg cgctacgtga gaagatgggc gagtttgccg agctgcgcga
    17941 tctgctggag cgagcaatca tcgacacacc gccggtgctg gtacgcgacg gtggtgttat
    18001 cgcatcaggc tataacgaag agctggatga gtggcgcgcg ctggctgacg gcgcgaccga
    18061 ttatctggag cgtctggaag tccgcgagcg tgaacgtacc ggcctggaca cgctaaaagt
    18121 tggctttaat gcggtgcacg gctactacat tcaaatcagc cgtgggcaaa gccatctggc
    18181 acctatcaac tatatgcgtc gccagacgct gaaaaacgcc gagcgctaca tcattccaga
    18241 gctaaaagag tacgaagata aagtcctcac ttcaaaaggc aaagcactgg ctctggaaaa
    18301 acagctttat gaagagctgt tcgacctgct gttgccgcat ctggaagcgt tgcaacagag
    18361 cgcgagcgcg ctggcggaac tcgacgtgct ggtgaacctg gcggaacggg cctataccct
    18421 gaactacacc tgcccgacct tcattgataa accgggcatt cgcattaccg aaggccgcca
    18481 tccggtggtt gaacaggtgc tgaacgagcc atttatcgcc aacccgctga atctgtcacc
    18541 gcagcgccgg atgttgatta ttaccggtcc gaacatgggc ggtaaaagta cctatatgcg
    18601 ccagaccgca ctgattgcgc tgatggccta tatcggcagc tacgtaccgg cgcaaaaagt
    18661 cgagattggc ccgattgacc gtatctttac ccgcgtaggg gcagcggatg atctggcttc
    18721 cgggcgttca acctttatgg tggagatgac cgaaaccgct aatattctgc ataacgccac
    18781 cgagtacagt ctggtgctga tggatgagat tgggcgcgga acgtccactt acgatggtct
    18841 gtcgctggcg tgggcgtgcg cggaaaatct ggcgaataag attaaggcgt tgacgctgtt
    18901 tgccacccac tatttcgagc tgacccggtt accggagaaa atggaaggcg tcgccaacgt
    18961 gcatctcgat gcactggagc acggcgacac cattgccttt atgcatagcg tgcaggatgg
    19021 cgcggcgagc aaaagctacg gcctggcggt tgcagctctg gccggcgtgc caaaagaggt
    19081 tattaagcgc gcacggcaaa aactgcgtga gctggaaagc atttcgccga acgccgccgc
    19141 tacgcaagtg gatggtacgc aaatgtcttt gctgtccgta ccggaagaaa cctcgcctgc
    19201 agtcgaggca ctggaaaacc tcgatccgga ttcactcacc ccgcgtcagg cgctggaatg
    19261 gatttatcgc ctgaagagtc tggtgtaata ataattcccg atagtctttt gctatcggga
    19321 atattaacga taactgacga atcaaataaa aataccctgt ataataggaa agcttatttt
    19381 acagggtaaa accatgccat ctacacgcta tcaaaaaatc aatacccatc actatcgcca
    19441 tatatggggc gttggtgata ttcatggtga atatcagtta ttacaatccc gcttacatca
    19501 actctctttt ttccccgaaa ccgacttact tatttctgtc ggcgataata ttgatcgtgg
    19561 gccggagagt cttaacgtcc tgcgcctgct aaaccagccc tggtttatct ccgttaaagg
    19621 caaccacgaa gcaatggcgc tggatgcgtt cgcgaccggc gatggcaata tgtggcttgc
    19681 cagcggtggt gactggtttt tcgatttaaa tgattcagag caaaaagaag ctacagatct
    19741 gttgctgaaa ttccatcacc ttccacatat tattgaaatc actaacgaca acataaaata
    19801 tgtcatcgca catgcagatt atccggggag tgaatatctc tttggtaaag aaatagcgga
    19861 gagcgaatta ctctggcctg ttgatcgtgt gcagaaatcg cttaatggcg agttacaaca
    19921 aataaacggc gctgattatt ttatatttgg acatatgatg tttgataaca ttcagacgtt
    19981 cgctaaccag atttatattg ataccggatc gccgaaaagc gggcggctgt cattttataa
    20041 aataaagtaa tgaggccggg taaagcaaag ccactacccg gcacttttta ttagcgctta
    20101 ccttccgcca gcagcggcgg gatgctcggc accattggcg cgtcatcaat atctttctgc
    20161 gtcatgcgga acgcttcggg ataatgttcg cggctggtac ggcgcagcgg ttcggtatcg
    20221 cgccaggtat aaaggcaatg ctggcactga tataccgtcc agacatcttt caccggcgat
    20281 ttcgccatca cttcaatctg ttcatcggca caacgtggac aaatcatctt gttctcctta
    20341 tttacgtgcg gccagcatag cggtcagttt ttcagcccag gctttggttt ccggcaaatc
    20401 caccaccggc tggctgtagt gaccacggtt gtccggggcg acaggcgtgg tggcgtcgat
    20461 aatcagcttg tcggtgatcc ccgccgggct tgagccaggg tcgagttcca gcacggacat
    20521 attcggcaac tgcaccaaat cccctgccgg atttactttc gaggagagcg cccacatcac
    20581 ctgcggcagg ttgaacgggt caacgtcttc atcgaccata atcaccatct ttacgtagcc
    20641 cagaccgtgc ggcgtggtca tcgcacgcag gcccaccgcg cgggcaaagc cgccgtagcg
    20701 ttttttggtg gagataatcg ccagcaggcc gtgggtgtac atggcgttta ccgcctgcac
    20761 ttccgggaac tcggctttca gttgttgata cagcggcaca caggtggctg gccccatcag
    20821 gtagtcgatt tcggtccacg gcatgccgag gtacagcgat tcgaaaatcg gcctggtgcg
    20881 gtaagagact ttatcgatac gcaccacggt catgttacgc ccgccggagt agtgcccggt
    20941 aaactcaccg aacggccctt cgatttcgcg tttgcggctt tcgataaccc cttcgaggat
    21001 cacttctgaa ccccacggca catcaaaacc agtcaatggc gcggtggcga tcgggtacgg
    21061 gctttcgcgc agcgcgcctg ccatttcata ctcagactga tcgtatttca gcggcgtggc
    21121 gcccataagg gtgatgatcg gatcgttacc cagggtgatg gcaatcggca gatcttcacc
    21181 gcgctcttcc gctttatgca gatgcagggc gatatcgtgc atcggcaccg gttgcaggcc
    21241 gagcttacgc ttgcccttca cttccatgcg gtagataccg acgttctgct tgccgaagtt
    21301 atccgggtcg agcggatcgc gggaaaccac gcacgctttg tcgagataga aaccgccatc
    21361 accgtcgttt aaacgaaaca gcggcaggat atcgaacaga ttaatctcgt caccatcgac
    21421 ggtgttctgc gcccaggctg gattggcgcg gcgctccggg gcgatcggga agttatccca
    21481 gcggcggata aactcatcaa tctgtttttt aaccggggtg tttggcggca ggcccaagga
    21541 aatcgcgtgg ttctgccagg aaccgatggt attcatcgcc acgcgggcat cggtaaaacc
    21601 gcgaatatta tcaaaccaca gcgccggtgc accatcgccg atacgcccgg tggcgttggc
    21661 agcggcagcc agatccggtt ccgcgttcac ctcttcgctg attttcagta actggccgtg
    21721 gtcatcaagc gcctgtaaaa agctgcgtaa atcatcaaat gccattattc attctcctga
    21781 gaaaaattcc gggcctgcgg caatccttgc cagcgcctgg cgtagggatg ttcaaggcca
    21841 aattgatcca gcacgcgggc taccacgtgg tggacaatgt catctaccgt ttcgggatgg
    21901 ttataaaagg caggcatcgg cggcaccatc gccacgccca tgcgtgaaag tgcgagcata
    21961 ttttcgagat ggatggtgct aagcggcatt tcacgcggca ccagcaccag tttgcggcct
    22021 tctttaagca cgacgtccgc cgcgcgccct accaggccat cagcgtaacc agcgcggata
    22081 ccggcgagcg ttttcatact gcacggaata acgatcatgc cgtctgtacg aaaggaacct
    22141 gaggagatgg tcgccgcctg atccgccggg ttatggctga agtcagcgag ggcagcaaca
    22201 tcgcgggcgc tgtaaggcgt ttccagttca atggtggttt tcgcccactt cgacatcacc
    22261 agatgagtct cgacattcgg catctcccgc agcgcttgca gtaatgccac accaagaggc
    22321 gcaccggtag cccctgtcat cccgacgatc agtttcattg cttactctct taattgttcg
    22381 tatacgaaca ttattattaa aatacgcctc tgttctcatt gcggcaagca ctgaaaagta
    22441 acaacatccc cttcgtcact gaaaaaccgc tatgatagcg gcagatagtt tgcccggagt
    22501 ttacatgacg ttacgaaata aagcgttcca tcagttacga cagctttttc agcagcacac
    22561 ggctcgctgg cagcacgagt tacctgacct gaccaaacca cagtatgcgg tgatgcgcgc
    22621 cattgctgat aagcctggta tcgaacaggt ggcactgata gaagcagcag tcagcaccaa
    22681 agcaacgttg gcagaaatgt tggcaagaat ggagaatcgt ggcttagtca gacgtgaaca
    22741 tgatgccgct gacaagcggc gtcgctttgt ctggctgact gccgaaggag aaaagatact
    22801 tgctgcggcg ataccgattg gtgacagcgt ggatgaggaa tttttggggc gtttgagtgc
    22861 agaagagcag gagctgtttg tgcagctggt gcgcaagatg atgaacacat aggatgcaaa
    22921 ttgccgggta ggacgctgac gtgtcttatc caggcgacaa aaacacaagc ttcccaaaca
    22981 aaagaaaaag gccagcctcg cttgagactg gcctttctga cagatgctta cttactcgcg
    23041 gaacagcgct tcgatattca gcccctgcgt ttgcaggatt tcgcgcaaac ggcgcaggcc
    23101 ttcaacctga atctggcgaa cacgttcacg ggtgaggcca atttcacgac ctacatcttc
    23161 cagtgttgcc gcttcgtacc ccagcaaacc gaatcgacgt gccagtactt cacgctgttt
    23221 ggcgttcagc tcgaacagcc atttgacgat gctctgcttc atatcgtcat cttgcgtggt
    23281 atcttccgga ccgttctctt tttcatcggc caggatgtcc agcaacgctt tttcggaatc
    23341 accacccagc ggggtgtcta ccgaggtaat gcgctcgtta agacgaagca tacggctgac
    23401 gtcatcaact ggcttatcca gttgctctgc gatctcttcc gcacttggtt catggtccag
    23461 cttatgggac aactcacgtg cggttcgcag gtaaacgttc agctccttta cgatgtgaat
    23521 cggcaaacga atagtacggg tttggttcat aatcgcccgt tcaatcgtct ggcgaatcca
    23581 ccaggttgcg tatgttgaga agcggaaacc acgttccggg tcaaacttct ctaccgcacg
    23641 gatcagcccc aggttgccct cttcgataag gtccagcaac gccagaccac gattgccata
    23701 acggcgggca atttttacca ccagacgcaa gttactctcg atcatccggc ggcgagaggc
    23761 gacatctcca cgcagtgcgc gacgcgcaaa ataaacttct tcttcggccg ttaacagtgg
    23821 tgaataacca atctcaccaa ggtaaagctg agtcgcgtcc aacacacgct gtgtggctcc
    23881 ctgcgataac agttcctctt cggccaaatc gttatcactg ggttcctctt ctactaaggc
    23941 cttttcgtca aaaacctcaa ctccgttctc atcaaattcc gcatcttcat ttaaatcatg
    24001 aactttcagc gtattctgac tcataaggtg gctcctaccc gtgatccctt gacggaacat
    24061 tcaagcaaaa gcctggttcc gccgatttat cgctgcggca aataacgcag cgggtttacg
    24121 gatttcccct tgtaacgaat ttcaaaatgc aagcgtgttg aactggttcc ggtgctaccc
    24181 atggttgcta ttttttgccc cgccttcact tcttgttgtt cccggaccag cattgtgtcg
    24241 ttatgggcgt aggcactcag gtaatcatca ttatgtttga tgataatcag attaccgtag
    24301 ccgcgcagcg cgttaccggc ataaacaaca cggccatctg cggtcgcgat aattgcctgt
    24361 cctttgctgc ctgcgatatc aatccccttg ttgcccccct cagaagcgcc aaaggtttcg
    24421 atcactttgc cctcagtcgg ccagcgccag gtggagatag gcgtactggt tgatgtactg
    24481 ctgacagtcg gctcggttgt gcttgctgtt ggtaccgtta caggcgctgt gaccgtggtc
    24541 gcagttggct tgttgttcgg caacattttg ttagcactct gttcacccga agactcagaa
    24601 tacgtaattg tcggttgcga cgcaacagca acggtggaat tttgtgcagg cttgatcaca
    24661 actccttgct ctgctgcgtc ggcctgggta atggcatttc cgccagtgat tggcgtaccg
    24721 gaagcattac ccacctgtaa ggtctgaccg acgttcagcg cgtatggtgc ctgaatattg
    24781 ttgcgctgag caaggtcacg gaaatcgttg ccagtaatcc aggcgatata gaaaagcgtg
    24841 tcgccttttt tcacggtata ggtactgccg ctataactgc ctttcggaat gttcccatac
    24901 tgacggttat agacgatgcg tccgttttcc atctgtaccg gctgctgagc tactggctgc
    24961 actggctgga tttgcggttg ttgagtagcc tgaatttgtg gctgctgcac cggctgaatt
    25021 tgcggttgct gcgctgtaga cgtcgtcccc attttcggcg gcggcgtaat caacatacca
    25081 gaattggtat gtgcaggcgc attgccatta acggagctga ccggtgccgg tggatttgaa
    25141 gtgtcagaac agcctgccag ccatagcgaa accagtgaca aagccgcaat gcggcgaacg
    25201 gtgaattttg ggcttcccgc gctcatttat cccccaggaa aaattggtta ataaccagtg
    25261 acataattac cgtgcaaggc accatactga acactggaaa agatgttcac gatacgctga
    25321 cctgcggcaa aataaccagg aaaaatccag gtatttcctc acgttttaag ccagctcacc
    25381 cttcactaaa gggacaaagc gcacggcctc cacggtatcg ataataaatt cgcctccccg
    25441 acgacgcacc cgtttcaaat actggtgctc ctcccctacg ggtaagacga gaatcccgcc
    25501 ttcgtccagc tgcgtcatta gcgcagttgg aatttccggc ggtgccgccg taacaatgat
    25561 agcgtcaaac ggcgcacgtg cctgccaacc ttgccatcca tcgccatgac gggttgaaac
    25621 attatgtaaa tcaagatttt tcaggcggcg acgcgcctgc cactgcaagc ctttaatccg
    25681 ttcaaccgag caaacatgct ggacaagatg cgccaggatt gccgtttgat atcccgaacc
    25741 ggtgccaatt tccagcaccc gcgactgcgg cgtcagctcg agtaattcgg tcattcgcgc
    25801 caccatatac ggctgcgaaa tcgtctgccc ctgacctatc ggcaaggcga tattgtccca
    25861 ggctttttgt tcaaacgctt catcaacgaa tttttcacgc ggcacggcgg caagtgcatt
    25921 cagcacctgc tcatcctgaa taccttgcgc acgtaattga tccagaagtg cttgtacgcg
    25981 tctgcttacc attgcgtgcc aactcccacg ctgtttaacc agtctgaaac cacatcttgc
    26041 gcgctatgcg cagttaaatc cacatgcagc ggcgtgatgg agacatagcc ctcatctacc
    26101 gcagcaaaat cggtccccgg accggcatca catttaccgc ccggcgggcc aatccagtac
    26161 agcgtattgc cgcgcggatc ttgctgcggg atcacctgat ctgccggatg tcgtgtaccg
    26221 cagcgcgtca cgcgaatacc tttgatttga tccaagggta aatccggaac attaatatta
    26281 agaatacgcc cggtgcgcag cggctcttta cacagtgcgc gcaaaattga acaggttacc
    26341 gccgcggcag tgtcgtaatg tttatgcccg tcaagcgaga cggcaagcgc cggaaaacct
    26401 aaatgacggc cttccatcgc ggcggctacc gtaccggaat aaataacatc atcccccaga
    26461 ttcggcccgg cgttaattcc ggacacaaca atgtccgggc gcggacgcat cagagcattc
    26521 acgccaagat agacgcaatc ggtcggggtt cccatttgca cagcaatatc accattttca
    26581 aaggtaaacg tgcgcaggga ggattccagt gtcagagaat ttgaagcgcc gctgcggtta
    26641 cgatcggggg cgaccacctg aacgtcagca aactcacgca aggctttcgc cagcgtttgt
    26701 ataccgggtg catgtacccc gtcatcatta ctcagcaata tgcgcataat cacctgttgt
    26761 gttgataagt tccctgacaa cgctggttgc aaaactaccc gccggaagcc agaaacggat
    26821 ttctacggtg acgtcatccc accaattcca gcttaattgt tgcggataca gcagcatcgc
    26881 tctgcgcgcg gcttcaactt tttcgcgcac cagtaaagct tgtaattcag tttctgcggc
    26941 gacagctgcc tgttcgaatg ccagcgcttc acgctgagtt ccccattcac cactgcccgg
    27001 caatgcggcg gttatcatca gctctttatc gttgacgcga cgctgtaatt ccaccagttc
    27061 ttcggtggtt gcaacaaacc agctaccacg tccggctaat tgtagcgcat cgccgtcaac
    27121 aacttgatta acgtctgctt ttttgaggcg ctcagcaaca atctgattaa acaacgcact
    27181 gcgggctgcc gacaaccaaa aactccgttt attgcgatcg cgcaccggag tattggtttg
    27241 cgcccagcgc agcgcgccct gcaagttgct accgccaatc ccaaaacgtt gggcaccgaa
    27301 gtagttcggt acacctttta cgcaaatatc gatcagacgt tgttcaacgt catcgcgatt
    27361 gctcacttcg cgcagaacca gggtaaaggc gttacctttc agcgccccta aacgcagctt
    27421 gcgcttgtgc cgcgcatact ccagcacctg gcagccttcc aattgaaagg cgctcagatc
    27481 gggcatttcc ttgcccggca cgcgagcgca taaccactgt tctgtaacag catgtttgtc
    27541 tttttgccca gcaaagctga cttcacgggc atgaattttc aggaatttcg ccagtgcatc
    27601 cgccacaaaa cgggtattgc agccgttttt gaggattcta accagaatat gctcaccttc
    27661 accatcaggc tcaaagccca aatcttccac caccacaaag tcttccggat tggctttcag
    27721 cagcccggtg ccttgcggtt taccgtggag gtaagtgaga ttatcaaact caatcatttt
    27781 gttgccttaa tgagtagcgc caccgcttca caggcaatcc cttccccacg tccggtaaat
    27841 ccaagttttt ccgtagtagt ggctttcacg ttaacatcat ccatatggca gccgagatct
    27901 tcggcaataa acacgcgcat ttgtggaatg tgcggcaaca tcttcggtgc ctgagcgatg
    27961 atagtgacat cgacgttgcc aagggtataa cccttcgcct gaatacgacg ccaggcttcg
    28021 cgtagcagct cgcggctatc agcaccttta aatgccggat cggtatccgg gaacagtttg
    28081 ccgatatccc ccagcgccgc cgcgccaagc aatgcatcgg tcaacgcatg gagcgccacg
    28141 tcgccatcag aatgcgccag caatcctttt tcgtaaggaa tgcgtacgcc accaatgata
    28201 attgggcctt caccgccaaa ggcgtgtacg tcaaaaccgt gtccaattcg cattatgtat
    28261 tctcctgatg gatggttcgg gtgaggtaaa actcggccag tgccaaatcc tccgggcgcg
    28321 tgactttaat gttatccgca cggccttcga ccaactgagg atggaatccg caatattcca
    28381 gcgccgaggc ttcgtcggta atagccgcgc cttcatttag agcgcgcgtc agacagtcat
    28441 gtaacagctc acgagggaaa aattgcggcg tcagcgcgtg ccataagccg ttgcgatcaa
    28501 cggtatgagc aatggcattt ttgcccggtt cggcacgttt catagtatcg cgcactggtg
    28561 cggctaggat ccctcccgtg cggctggttt cgctcaacgc caacaatcgc gcgaggtcat
    28621 cctgatgcag acaaggacga gcggcgtcat gcaccaatac ccactgcgcg tcgccagcgg
    28681 ctttcaaacc tgccagcacg gaatcggcac gctcatcacc gccatctaca acggtgattt
    28741 gcggatgatt cgccagagga agttgtgcaa aacggctatc gccaggactt atggcaatga
    28801 cgacacgttt cacccgggga tgcgccagca gcgcatacac cgagtgttca agaatggttt
    28861 gattaccgat tgagagatat tgcttaggac attccgtttg cattcgacgg ccaaatccgg
    28921 ccgccggaac cacggcgcaa acatccaaat gagtggttgc catgttaatt cccgggctga
    28981 tttatcgatt gttttgcccc gcagactgtg cgcgcttcga cgcgtcaggc accagacgat
    29041 aaaaagtttc gcccggcctg gtcatgctga gttcattacg cgcacgctct tcgagcgcct
    29101 cctggccgcc attgagatcg tcaatttcgg caaaaagttg atcgtttcgc gctttaagtt
    29161 tcgcgtttgt agcttgctgt gccgccacat catcattgac gcgggtatag tcatgtatac
    29221 cgttcttacc gaaccacagc gaatactgta gccagaccag aatagccagc aacagcagcg
    29281 ttagtttacc catcctgccc cctgaaaaac ggcatcatca tcccatgcat ccgaagacga
    29341 ctctacatcc tctgttgggg ataccgcgac aacgcgggca aatgtaccac atttgtccat
    29401 tgttacgtat acccagggcg tgcagaacat aatctcatta ttagttacgg tttgaattat
    29461 gaacagagga gacaagaaag tacaaattag cccagtagcc acataaacag tgcgccaaac
    29521 ataatgccta ctgtcatcag ggtgaaaaca atactgtagc gtagctttcc gtccatcaat
    29581 gaatgcagcg caatccccac cactaccgca acgggcatca gcgccagaaa gaaaggccag
    29641 gtgtagataa agaagaacag cgtgttagag ccataaatca acatcggcat cgccagcgca
    29701 aataaccagg agataaaacc gaccacggca ccaggcagtg accatgtggt ttcttcatcc
    29761 tcagtaaggc tgtcgttatt tgttagtgta atgttatggc tattacgcat atttgatcct
    29821 gttactttga cgaaccgggc atggaaaccc ggtggtgtct caggatctga taatatcgtt
    29881 ctgtctcaac agatctaata attgctgtac caaatttgtt actaattgtt caccattgag
    29941 atgaatttct gccgattcag gcgcttcgta aacggaatct attcccgtaa agttgcgcag
    30001 ttcaccggca cgcgctttct tatataagcc ttttggatcg cgggcttcgc aaatcgccag
    30061 cggcgtatcg acaaacactt cgataaagcg cccttctcct acgcgttcgc gaaccatctg
    30121 gcgttcggcg cggtgtggcg agataaatgc ggtcagcacc accagtccgg cttcaaccat
    30181 caaattcgcc acttcaccga cgcgacggat attctcttta cgatcggcat cgctaaaacc
    30241 gagatcgctg cataatccgt ggcgaacatt gtcgccatcc agcagatacg tactgacgcc
    30301 gagtttatgt aacgcctcct ccagcgcccc ggcgaccgtt gatttaccgg acccggagag
    30361 gccggtaaac cacagcacta caccacgatg accgtggtgt agctcgcgtt gttgcacagt
    30421 gaccggatgg ctatgccaga cgacgttttc gtcatgcagc gccattattt ctcccccagc
    30481 aaatcgcgcg cgccccagtg tggaaagtgg cggcgaacca gggcattcaa ttccagttcg
    30541 aatgcactaa attcagatgg cgcagcagtt gcctggctaa ctggctcatg caccataccg
    30601 gcacctacgg tcacattgct caggcgatcg ataaaaatca gcccaccggt aaccgggttt
    30661 tgctgataac gatctaacac cagtggctcg tcaaaagtga gatccaccag gccgatgccg
    30721 ttcagcggca ggttttcaac ttcgcgttgg gtaaggttat taatatcaac ctgataacga
    30781 atgccatcaa cacgagaacg cgtcttctta ccggcaattt tgatgtcgta actctggccc
    30841 ggggaaagcg gctgttccgc catccatacc acatccaccg acgcgctctg cacagctggt
    30901 aacgcttcgt ctgccgccag cagcagatcg ccacggctga tgtcgatctc atccgtcagc
    30961 accagggtga tagcttctcc ggcaaaggct tcttcgcgat caccatcaaa agtcacgatc
    31021 cgcgcgacgt ttgattccac accagagggc agcactttta cacgttgccc gacttccacg
    31081 cgaccggatg ccagcgttcc ggcgtagcca cgaaaatcga gatttgggcg gttaacgtac
    31141 tgcaccggga agcgcattgg ctgggcatcc accactcgct gaatctccac ggtttccagc
    31201 acttcgagca gtgtcggacc gctgtaccac ggcatacttt cactttgcga agccacgttg
    31261 tcaccttcca gtgcggagag cggcacaaag cggatatcca gattacccgg cagctgcccg
    31321 gcaaaggtca gataatcttc acgaatacgg gtgaacgtct cttcactgta atccaccaga
    31381 tccattttgt tgatcgccac gaccagatgt ttgatcccca acagtgtgga gataaaactg
    31441 tgacgacggg tttgatcgag cacgccttta cgggcatcga tcagtaagat cgccagttca
    31501 catgtcgatg cgccagtcgc catattgcgg gtgtactgct cgtgccctgg agtgtcggcg
    31561 ataataaatt tacgcttctc ggtagagaaa tagcggtagg ccacgtcaat ggtgatgccc
    31621 tgttcacgct cagcttgcag gccgtccacc agcagagcca gatccagctt ttcgccctgg
    31681 gtgccgtgac gcttactgtc attatgcagc gatgagagct gatcttcata gatttggcgg
    31741 gtatcgtgca gcagacgacc aatcagggta cttttgccgt catcgacgct accacaggtc
    31801 agaaaacgca gcaggctttt atgttgttgc gcaatcatcc aggcttcgac gccgccttca
    31861 ttggcgattt gttgtgcaag tgcggtgttc atcttaaaaa tacccctgac gttttttcag
    31921 ctccatagaa ccagcttggt cgcggtcaat cacgcgcccc tgacgttcac tggtggtgga
    31981 aaccagcatc tcttcgatga tctccggcag cgtttgtgca tttgactcca ccgcaccggt
    32041 cagcggccag cagcccagcg tacggaaacg caccatccgt tttttaatca cttcgcccgg
    32101 ttgcaggtcg atacggttgt catcaatcat catcaacata ccgtcgcgtt ccagaaccgg
    32161 acgttccgca gcgagataca gcggcacaat gtcgatattt tccagccaga tgtattgcca
    32221 gatatcctgc tcggtccagt tagagagcgg gaaaacgcgg atgctttcgc ctttgttaat
    32281 ctgcccgtta tagttgtgcc acagctccgg gcgctgattt ttcgggtccc agcgatggaa
    32341 gcgatcacgg aaagagtaga tacgctcttt agcacgggat ttctcttcgt cgcggcgcgc
    32401 accaccgaag gcggcatcaa aaccgtattt attcagcgcc tgcttcaggc cttcggtctt
    32461 cataatatcg gtatgtttcg cgctgccgtg cacgaatgga ttaatcccca tcgccacgcc
    32521 ttccgggttt tgatgcacca gcagctcgca gccgtaggct ttcgccgtac gatcgcggaa
    32581 ctcatacatc tcacggaatt tccagccggt atcgacatgc agcaacggga aaggcagcgt
    32641 acctggataa aacgccttgc gcgccagatg cagcatgacg ctggaatctt taccgataga
    32701 gtagagcatc accggatttg agaattctgc cgccacctcg cgaataatgt ggatgctttc
    32761 cgcctccagt tgccgcaggt gagtaagtcg tatttgatcc ataaccgttc ctttgcaata
    32821 ccgctatttt cttgccatca gatgtttcga ctatagggag cgtaagagaa cgaatgaaat
    32881 taccaattag aatgagtagt tccttaacgg aataacgatt tggcaaagct aatatcaaaa
    32941 agtgcttaag gcaccggatt tcgggcgttt aggaagattt gaaattgttt tagcgcagcg
    33001 gcagtttcat actatggcgg taaaaaaatt tgcatggtat ttaaggactc actatgtttt
    33061 ccgcattgcg ccaccgtacc gctgccctgg cgctcggcgt atgctttatt ctccccgtac
    33121 acgcctcgtc acctaaacct ggcgattttg ccaatacaca ggcgcgacat attgccactt
    33181 tctttccggg acgaatgacc ggaacacccg cagaaatgtt atctgccgat tatattcggc
    33241 aacagtttca gcaaatgggt taccgcagtg atattcgtac gtttaatagc cgatatattt
    33301 ataccgcccg cgataaccgc aaaaactggc acaacgtgac gggaagtacg gtgattgccg
    33361 ctcatgaagg caaagcgccg cagcagatca ttattatggc gcatctggat acctatgccc
    33421 cgcagagcga cgcagatgca gatgccaatc tcggcgggct gacgttacaa ggaatggatg
    33481 ataacgccgc aggtttaggt gtcatgctgg aactggcaga acgcctgaaa aatacgccta
    33541 ccgagtatgg tattcgattt gtggcgacca gtggagaaga ggaagggaaa ttaggcgctg
    33601 agaatttact caagcggatg agtgacaccg aaaagaaaaa tacgctgctg gtgattaatc
    33661 tcgataactt aattgttggc gataaattgt atttcaacag cggtgtaaaa acccctgaag
    33721 cagtaaggaa attaacgcgc gacagggcgc tggcaattgc gcgtagtcat ggaattgccg
    33781 caacgaccaa tccgggtttg aataaaaatt atccgaaagg cactggatgt tgtaatgacg
    33841 cagaaatatt cgacaaagcg ggcattgctg tactttcggt ggaagcgaca aactggaatc
    33901 ttgggaataa agatggttat cagcaacgcg caaaaacagc cgcattccct gcgggaaata
    33961 gctggcatga cgtaagactg gataatcagc aacatattga taaagcactt cctggaagaa
    34021 tagaacgtcg ctgccgtgac gttatgcgga taatgctacc gctggtgaag gagttggcga
    34081 aggcgtcttg atgggttgga aaatgggagc tgggtgttct accgcagggg cggggaattc
    34141 taagtgatat ccatcatcgc atccagtgcg cccggtttat ccccgctgat gcggggaaca
    34201 cagcggcacg ctggattgaa caaatccctg ggccggttta tccccgctgg cgcggggaac
    34261 acaaaccgaa acacacgatc aatccgaata tgagcggttt atccccgctg gcgcggggaa
    34321 cactttggtg acagtttttg tcactgtttt ggtgacggtt tatccccgct ggcgcgggga
    34381 acacactaag catacatatc tgtttttaaa caaatttatt ccacatcaac aatctaccaa
    34441 ctaaattcaa acatttcctt atttttaaag aacacataac ctattgatta tcaacaggaa
    34501 gaaaagcaac caaacgtaac ccatccaaat ccaccggaat acgtctgttt tctccccagg
    34561 tctgaaattc aaaacccgac tcggtattgg tcgcccaggc catcaccaca tttccgcaac
    34621 cagccagttg ggtaatttgc tgccagatca tctcccgaat acgttttgat gtatcaccaa
    34681 catacacacc ggcacgcact tccagtagcc agattgcgag ccgtccacgt aagcgcggag
    34741 ggacattttc tgtaacaacc acgaccatgc tcatccgccg cgcccccggt gaccactatc
    34801 acccagcgtt tcaggttcag ggatggcagg cggtaacata tccggcgcgg gttgtggtgg
    34861 ttcaatttca cctgcagcaa ggactttctc aattaacggt attaatttgc ccgttaactt
    34921 agtgctacgg aaaatatcgc gacaggctaa tctgacttct ttatcaggtt ctgcgggttg
    34981 cctcgctgct atttcaaatg cctttggcac aaccgaatca aatttaatga tatcggctat
    35041 gtcataaaca aatgaaagcg gtttgccact atgaataaat ccaatagcgg gcgcatatcc
    35101 cgcggctaat actgccgctt cagaaatacc gtacagacat gatgtggcag cactgatgca
    35161 gcgattcaca acatcgcctt tttcccagtc tttaggatcg tatttgcgac cattccattt
    35221 cacaccatat tgtttcgcca gtaatgcata ggtctggcga acgcgggatc cctcaattcc
    35281 ccgtagctga tccactgaac ggcgagctgg cggtggctca cgaaaacgta attcatacat
    35341 tttgcgcacc accttcaggc gtagatcttc cgttaaagcc agctttgcct ggtagagtaa
    35401 tttatctgcc cgcgcccctc cgggttgtcc ggaagagtaa acgcgaacgc ccgcttcacc
    35461 gacccagacc agcagtgttc ccaccgtggc ggccagatgc accgccgcgt gggaaactct
    35521 cgttcccggt tcgagcataa tgcaggcgac cgatcccacc ggaatgtgcg tgcggatccc
    35581 ggttttgtcg atcagcacga aagcgccgtc cagtacgtcg atttgaccgt actggaggaa
    35641 gatcatagag gtgcgatctt ttaacgggat cggactcagt ggtacaaacg tcacacctct
    35701 gctccgggtt tgatcagcat cagaccacag ccgaacgcgc ggcttttgcc atacccctga
    35761 ctgagacgtt gcagaaataa cccgggatcg gtgaccgtaa gcatccccgt ataatccacg
    35821 ctactgaact ggatcagttg ccgggagttt tcccgccgca gttgctgttg tcggtaggca
    35881 tcaaccgagg tatccagcag tgtaaaaccg cttctctccc cctgcgctgc cagccagtcc
    35941 agcgccgcct gttgttgatg caaccagaca tcacttcctt ccgcctgccc cctcacctgc
    36001 cgtttcgcct ccatcagcag atcgtggcgc ttgcccgctt tacagattgt tggattcgcc
    36061 cgcaggttga aacaaagttg ttgtccggta cgcagctcgg gcacaaatga ccggcattcg
    36121 atggtgaacg tttcactttc cgccgggcgt tcctgcgaca gtacaaaaaa gcgaaacgcg
    36181 ccctggagtt cttcccgacg ataaagaaat tgcctttctt tgccgccagg gaagagatcc
    36241 cacagccact gatgcatcac atattccccg cgatccacca aatgcagcaa ctgcgcaggc
    36301 gaaagctggc cggtatgtaa ggttattctt gagaggtaca tggttcctcc ttgctgagcc
    36361 acggcccctg attgatggtg cgttccccaa acagccactg ctgacgattt aaaggaacat
    36421 cccggcgacg taatattttg ctcgcgacca ggccgtcgtg ttccccttcc caccagcatt
    36481 catcctgaag tttcgggagt gagactttca gttcgcggaa actatcctga tactgttggt
    36541 atgcgttacg taagacatca gacgcgttgc cttcgagcag taacggcgca agtggtaacg
    36601 ccagcggatg actttttcgc cccagataaa gcggaaaaac cggatgacgt aaaccgtcct
    36661 gcaactgttc aaggctgtaa ggcgcatcgg gggttgttgc caccgccacc atccaccagg
    36721 catcggtgta gtagtcgcgc cgggagataa tcgcgctcag aagatcaggg gcgctcaact
    36781 cttcgcgacg gctgaaataa cgcgctttac gcacctcttt tggcatctgg accgtgtgat
    36841 aatcccgtgc ccagcgcggg ttacggctgg cgcaaaccac cagtgaatag tggcggttaa
    36901 acgcgtttaa tcgttcggta tcatcacgcc gaatccctac cccggcagcc agcagcccca
    36961 gcaatgctga gcgcgaaggc agttcatggg tatgacgcac ttcgccgggg gcatcgacgc
    37021 cccaggatgc cattggccca tgaagctgaa aaatcaaata ttggctcatt agccgcccct
    37081 tacgcgcaga taaagtccag cacgtccttc atgcttccct gtttgttcat cacgtcaaag
    37141 cttgcgcatt cggtcttctg ttcatagacc gtattcatat tttcgcgaag cgttgtaata
    37201 cgctgcaccg ccacatctaa ctgccgggtg ccattaatgg gttcatagaa agccgccgcc
    37261 agagaacgtg gttgttcggt gcctttttct gccagcgccc aggaggcgta ggcacggctg
    37321 gcaaagctgt tctgtttgcc ggttggggag actttaagtg cggcttccgt aaaggcgcgc
    37381 aaggtctgat tagctaacgc ttcgtcaccg ccgaggtttt cgaccagcag atctttatcg
    37441 atgcagatat aggtgtagaa cagcgcagaa ccgaatccgg tttcaccaag atgcccggca
    37501 ccggcatctt cagaagcctg gcgcaaatca tcaacggcgg tgaaaaaatc atcttcgaca
    37561 atcgtttcac tgacaccaaa tgcatgcgcg acctggcagg cggcttcaac attaaattcg
    37621 ggtttattcg ccagcatacg accaaacata gcgatatcta ctgccatgcg atctttacgt
    37681 aacaaagcga gatcttcctc ttttggcgcg cgcttttctt cggccagttg atgggccagc
    37741 gcttttacgg cgtcaaattc tgccgggctg atatggacta attgttcagt ttcggcgtta
    37801 gtgagcggat cttttggttt tttgtcgttt ttagctttcc caagataatc cgcaattttt
    37861 gccgcccatt cgatggcttt tttctcttcg atgcctttct caatcaggat agttgccgcc
    37921 tcacgcgcaa tacgcccact gcgaatacca atatggcccg ccagtgcctg ttcaaaaagt
    37981 gcagaagtgc gccacgcacg tttcagactt tgcgaggaaa cgcgcagtcg cgttgctcca
    38041 cccaggacca cggttttcgg cgctccggtg tcatcacggt taaggttggc tgcagggtaa
    38101 gcggttaaca aatgaagctg aataaatgtc gtcatgagat agtcctttat tgattttgtt
    38161 cgttatcgac gtcgccagcc tggtaatatt ccagcgccca gcggatacgg ataaattcgg
    38221 ttggccgctg atgacggcgg tgatgattga gcagatcgtc gctctcctgg caccagcgga
    38281 agacaccctc tgccagagag tcaagattaa cggaaccgtt taataatctg actacgcggc
    38341 gtaactggcg cagtaattca tccggtgttt ttactgccga caggcgagta aaacgcccct
    38401 ttgacattac cgccgccagc tgcgcagcaa aaggcagccg ttcatcaatg gctttaacat
    38461 tagcgctaag tgcggctatc agcgccagtg cggtaatacg ccattcaggt tcatcctgcc
    38521 actttatttg tctgttcttc agaaacaggc gaaatccatc cgtcagacaa acatcattaa
    38581 ccgtcgtact acgccgcaga ctggcacgtt cgccgcgttt ctcctgcaat tcctcatgcc
    38641 atttgcgcag cgtggctttg tgctcctctt ttacaatact cattcagcag cctcctgctt
    38701 tttctccctg gcggctttag cactttgctt ctccgccgat gttgtaaaat atttcttgcg
    38761 cgcggtcatg acgcgttcca aatcaacggg ctcataagga ttggtgaata cacgctcgtc
    38821 aaaatcctga cgtgcgaata accaaatttc cttttgccat ttgccgagta attcatccgc
    38881 atcctgacct tcttcaattt ggcgcactaa cctcaggaag cgatgctgag ttttgttcca
    38941 gaagtcgata tccacaaaac tgaaatcacc ccttgcacct tttggatcgg agaaccatgc
    39001 ttctttcaat gcactccgta acagactcag aatccgtgaa gccgtttgcg cagccagccg
    39061 cagcttcggt atctggcctt cttttttatt gagcagcagc gggaaatggt gttcgtacca
    39121 acagcgcgct ttcatgttgt cgaaatcata accaaatccc cacaggccca cttttgcctg
    39181 tttcagactg ctggcattaa agagtttcac caccagcgcg ggaagttccg tattgttttc
    39241 tgacttaccc gtttcgataa ggcctaacca gtcgcgccag attaaaccgc ccggttgtgg
    39301 tttaacggag taaaactcac cgccctcttt aagtggtaca cggtaaggcg ttaagggatg
    39361 ctgccacatg gcataattcg caccgtaatt tttggtagtc atcaaactca gaagcgcgtc
    39421 actctgctca ccgcaaatat cgcagttgcc gactgtcgtg gtattaaaat caatacgaat
    39481 acgccgcggc attccccagt acgcctggag tttattgacc tgatcatcgg ttaccaccgc
    39541 accggccagt tcgctggtac gcgtcgggcc aagccagggg aaaaccagat cgtcaaattt
    39601 tttgggtagc ggtaagtcgg cttcatcctg cggcatcacg ttgagccaca gtttgcgcca
    39661 caagggggct tgttgattgc cctgatactc ctgcaattca atcagagtcg tcatcggccc
    39721 accgccgcgt aaaccggtgc gatagccttt gccacctgac ggcgcattta actgtaggga
    39781 gaacagagct aacgcagaac aatgagagca tacgtgttca gtcacgccac gcttaataaa
    39841 gtggtcttta ttaaacttcg ttgtttgagc gccgggaatc tcaggcagta gcgaagcgac
    39901 ctgaacttta tcgcccatga gcacctcgaa atcctgcata aatgaaggtg aatctgggcc
    39961 aaactggaaa gggtgttcta atgacagcaa tgcttcccgt agcttttcag cttccagccc
    40021 gtcttcccag atatcatccc aacgacgata atcttttggc gcgaaactgc tttgtagtaa
    40081 ccccagcaaa aactgccatg ccgccccctg gagatctgcc cgcggcgcag cgatatcgac
    40141 aacattttca tccgccagat cgactggcgc cagcttgcct gttgttccgt ctttaaaacg
    40201 aacgggcaac cacggggttg tcagaagtga aaacgagttc atatgtatcc tctcgtaaaa
    40261 aagaccatcc ctggtcggtt gtccatcatt ccatcctttt cggtgtatca actattcaga
    40321 gagtcctgtc gcaggtatct caatcaacct tgccaatcaa tccctccctg gccgaatacc
    40381 cgcaactttc gtcatcagtg actaaaatca cgtttgccat ttccggatct tgccgctgtt
    40441 caatgcacca ctgcctgaac gcttcccctt ccagtaaaga aaactcatcc cgatgttttt
    40501 tccaccagct tcgacgcact ctgacaacgc tcatttccca tgcgtgagca ccggtggcat
    40561 aaggcttcac cacaccggca atacaggtag ccagccacag ggaaacagat tcctcagcca
    40621 gacgtgtcga cagcttttcc ggaaggtaat cgttgatatt ggcggcatag ccaggcttga
    40681 agttcaggac aaacttttta gccattgcgc gatcgcagta atatttgccc acttgctcct
    40741 gctcgctgcg ggcaaatcct tccggcatta ccacgtcctc accgtagact gattcaataa
    40801 gaaggcgggc tgcgtgtggc atttgaatag cgccttgctc acgcagtaca cgctgcgtca
    40861 gccagattcg tccatgatcg ggatagacat atgcactgtt acgcatggca ctgccgaacc
    40921 attcgtcacc aggagcgtcg tcccagacgg gggccagaat cagcaattca ggaggggaac
    40981 gctcgtcttt tccgtcacgc tttaactgac cattaatatc gcggatatgc cgctgtaatc
    41041 gccccgctcg ctgaatcagc aaatcaacag gggccaggtc ggagatcatt tcgtccaggt
    41101 cacaatcaac gctctgctct aagacctgag tacaaatgag gacttttccg gcacgctgtg
    41161 aaccgtcttc tttaccaaag cgtgccagcg tctccatttc aattcgctgg cgatcgctaa
    41221 aagcaaagcg gctatgaaag agtgaaaggc tggaagcggg aatgacgccg cgggcaagca
    41281 gctgacgatg aaccttaata gcgtcatcga cagaattccg gatccaggcg atgcattttc
    41341 cctgacttac cgccgattcg atacgcgcaa tactctcttg ttcactatga agccaaccca
    41401 cgctgacgct acgctcaacg tctttgcgcg tcgctacccg gtgtgagttc acatcggatt
    41461 tcgtgacatg cgtcagccag gggtaatcat ccttttcaag gaacggagct tcttgctggc
    41521 cctctgtgcc acgcgcaaag gcggcgacga gtttgtcgcg ctgctgttgg gataacgtag
    41581 cagaaagcaa aatgacgctg tttccgccac gcgcctgccg ctcgatcagc ccttcaagaa
    41641 tgcacgacat gtaagcatca caggcatgga tctcatcagc cagcaggatt ttgttactca
    41701 accccagaag ccgcagatta ttatgtttaa acggcatcac tgccatcatc gcctgatcca
    41761 gcgtgccgac gccaatttca gccagtagcg ccttcttgtt actgttggca aaccaggccg
    41821 cacatccctg actgaatgtt tgttcatccg gttcttctga cccgactaaa tcaccggacc
    41881 agagtgattc attgaagcgg tccattaatg tgcgggcact gtgtgccagc accaagctgg
    41941 ggcgggactc tggcgaatag aaagcaagcc aggttttgac cagccgatcg tacatggcat
    42001 tggccgttgc cattgttggc aggccaaaaa acaaaccctg tgctttcctc gcagccatca
    42061 acctgtgcgc caggataagc gccgcttctg ttttacctgc gccagtcacg tcttccagaa
    42121 taaataactg tggccctggc tggctgatat ccagatccag taccttttgc tgtaatggtg
    42181 tcgggtgctc aataaaagga aacagcgtat taattccggt gaaaggtgcg gtttctgctt
    42241 ttggaggaaa gacggttaag gcgttttgag cctgaactaa agttttctgc cagtaatctt
    42301 taatatccat tgggtgtgcg acgcgtggaa aaaatcgcgt tgacgaaccc gtccagtctg
    42361 cgagtacgac tgttgcagag atataccagg aaagttgttt taaaagttca acgccctcgt
    42421 catcatccca gaatgtggga atctctatga gcggaaacag tgccttgatt tcaaggagaa
    42481 aatctcgcgc ggcagctttg tcttcaggca gaaaattatc cagctcatca atacggtcag
    42541 gtggtcgacc atgatgcccg gtagttatgg acatccacat ctctattaca cgtgtaagtt
    42601 tacgagaaga gagtgaagat gaaggaagca actcctcaca ttcacttaaa taataattcc
    42661 acagccagta acccagcgtt gaatgagaga tcttttcgta attctttctg gaaccttccg
    42721 gaatcttgag ttcaggggcc aggtaaagtt gctgaaaaga gcgggcaaat tttccaatat
    42781 cgtgccagca cagcaaccaa gcgaaaaatt gagccgcctg ttccttgtca gaaatcccta
    42841 attgacgaaa gtaatcagcc agcccgaagc aatttctttt aaccattaaa tagcccattg
    42901 cggccacatc cagcgaatgc cagcaaagaa ggtgatagcc gtcgccaccc tctttctcgc
    42961 cacgtcgggt ttttccccag aaatcaaaga aagtcacaat atttttatcc ttcagtaaac
    43021 ttaaaggata tttacgcata ttttaaaaat tatctgtgat atatatcagc caataaacat
    43081 ttatattatg aataaattaa tgattttcat ttgaaattca taatgataaa tagagactat
    43141 atattcacat aaaaaatata aatttaaaaa agggtgcagg agaccaaacc atcacaattc
    43201 aatacgcggg gttttacccc cgcgtggtgg ctacttcttc gattcgtaag cgagaactgc
    43261 cttaaactcc ataccacgct ctctgatact caactcacat aacgagtcgc ctaccatgag
    43321 cgtgaatccc agtactgtac aacacagtac tatgattgcc acaagggcat attttgttag
    43381 catgtcttgc ctccctgcaa agaagagact aacatccgaa ttgctacgtt cgaacgcgag
    43441 cctcaggttg atagaaatat cgcctggggc ttttgtccat ctggaacctc gcgaatgctt
    43501 aacgccagac agcctcaagc acccgacgcc attctatacc tgataattct tccagcggcg
    43561 ttcacgttta aaatcaggaa tgctgctcgc aaaataaaaa cgcccccatc attactgaca
    43621 gaggcgttta atttatccgg caatatttac ccttcgtgta acccacattc cctttttaag
    43681 ccaaagaaac gcgtttcttc ttccgccatg ccgggttccc atttacgggt tgtatgggta
    43741 tcgcccaccg ataaatatcc ttcgtcccat aatgggtgat atttcaggcc attcttttgc
    43801 agatactgat atatcgttcg gttatcccag tcgataatcg gcagcacttt aaatacgcca
    43861 cgctgaattg ccagcaccgg taaattggcg cgactgccgg attgttcacg gcgcaggcca
    43921 gcaaaccagg tttgcgcgtt aagttctttc agcgcccggt tcatcggttc gactttgttg
    43981 atgtcattgt acttttcaat gccttcaacg ccctgttccc acagttttcc gtagcgtgct
    44041 tcctgccagg ctgcgctttc ggtagcacgg tacactttca ggttgagctt gagtttgtcc
    44101 gttaactcgt caataaagcg gtaggtttcc gggaacaagt aacccgtatc ggtgaggatc
    44161 accggaatat ccgggcgaat ttgattcacc agatgcaggc tcaccgccgc ctgaatgcca
    44221 aagctggaag aaagtacata ttcaccgggc agattatcca gcgcccaggc tacgcggcct
    44281 tcagcgtcca gtttttccag ttgggcgtta gtttccgcca gcgccagaat gcgatctacc
    44341 ttcggcagtt cgttcagggc gtttagatcg agtttggaca tagattcctc actgtttgcc
    44401 ttgcctgatg cgacgctcac gcgtcttatc aggcctacaa gaccgggctg atggttaatc
    44461 ccacaaatcg cgcgccggat cgagcaccgg gcgaatgatg cccgcacgca ccgtaaagtc
    44521 gccgaagcct tcacccgctt cgcgctcttt cgcccagcgc cctatcagtt catcaagcga
    44581 cgccaggatt tccggctcgg tgatgttttc tttatacatc cgtgggatac gtgtcccaat
    44641 gcggttgccg ccaagatgca ggttgtagcg acccggcgct ttacccacca ggcccacttc
    44701 cgccagcatc gcgcgaccac aaccgttcgg gcagcctgtt acacgcatca cgatatgctc
    44761 atcgctgaca ccatgtttcg ccattaaatt atcgatgttg tcgataaaag acggcaggaa
    44821 acgctccgct tccgccatcg ccagcgggca agtcgggaac gagacgcagg ccattgagtt
    44881 ttcacgctgc ggcgtgacgg cattcattaa cccgctctct ttggcgatct tctcgatctt
    44941 cgctttctcg ctttccggca caccggcgat gatcagattc tggttcgccg taatgcggaa
    45001 atcacctttg tggatcttcg cgatctccag caggccggtt ttcagcggac gccccggata
    45061 atcaaggatg cgaccatttt cgataaacag cgtcaggtgc cagttatcat caatgccctt
    45121 aacccagcca atacgatctc cgcgtccggt gaactcatac ggacggatcg gttcaaattt
    45181 gatccccgcg cgacgctcca cttccgcttt aaacgtctca acccccacgc gctccagcgt
    45241 gtatttggtt ttggcatttt tacgatcggt tcggttaccc cagtcacgct gagttgtcac
    45301 gacggcttcc gccactgcca gcgtatgctc cagcggcaga tagccaaact cactcgccgt
    45361 gcgggcgtag gttttcttgt tgccgtgttc gatagaaagt ccaccgccca ccaacaggtt
    45421 aaagcccacc agcttgccgt tttcggcgat tgccacgaag ttcatgtcgt tggcgtgcag
    45481 atcgatatcg ttctgtggcg ggatcactac cgtggttttg aatttacgcg gcaggtaggt
    45541 ctggccgagg atcggttctt catcggtggt ggcgactttt tcctgatcga gccagatctc
    45601 cgcatacgcg cgggtacgag gcagcagatg ttcagagatt ttcttcgccc actcgtacgc
    45661 ttctgcgtgc agctgcgact cgtaagggtt cgaggtgcag agtacgttac ggttcatgtc
    45721 gttagcggtc gccagcgcat caagaccgac cgagtgcagc atctggtgca ccggtttgac
    45781 gttctttttc agaatgccgt ggaactggaa tgtctggcgg ttggtcaggc gaatgctgcc
    45841 atagatggtg ttttcaccgg caaatttatc gatcgcctgc cactgtttgg tggtgataac
    45901 tccacccggc agacggcagc gcaacagcat cgcatggcgc ggctccagct tctgttcagc
    45961 acgttcggcg cggatgtcgc ggtcatcctg ctgatacatg ccgtggaagc ggatcagcag
    46021 gaagttatcg cccttaaagc cgccggtcag gccgtcgttt aaatcttccg caatggtgcc
    46081 gcgcaggtag ttgctttcaa gcttcatgcg ctcggcgtct gtcagttttc cttcgaccac
    46141 taaaggccct ggatgttttt cgctcattag tagacatctc gctgataacg gcgctctacg
    46201 cgcagctcac ttaaaaattc atccgccgct tcggtgtcca tgccaccaaa ttcggcaatc
    46261 acttccagaa gtgcctgctc aacgtctttc gccatgcgat tagcgtcgcc gcagacataa
    46321 atgtgggcac cgtcattgat ccagcgccac agctccgcgc cctgttcgcg cagtttgtct
    46381 tgtacgtaaa ctttttcttt ttgatcgcgc gaccaggcaa gatcgatacg cgtcagcacg
    46441 ccctctttga cgtaacgctg ccactccacc tggtagagga aatcttccgt aaagtgcggg
    46501 ttgccaaaga acagccagtt tttacccggc gcttcgtcag cggcgcgctg ctgcataaag
    46561 gcgcggaacg gcgcgatgcc ggtgcctggg ccaatcataa tcaccggggt ttccgggtta
    46621 gcgggcaggc ggaagttatc gttatgttcg ataaatacgc gaacttcgcc ctcttcttcc
    46681 acgcggtccg cgaggaagct ggaggcacca ccggcacggg cgcggccttc cacgtcgtaa
    46741 cgcaccacac caacggtgac gtgtacttcg ttctcgactt cctcctgcga ggaggcgatg
    46801 gaatacagac gcggcgtcag cgggcgcagc agattaatca gcgcttcggc gtccagttgc
    46861 gccggagaga agcgcaccat gtcgacaatc ggcgtagtcg cggcgtaatg ctgtaacttc
    46921 gctttatcgc ccaccagcgg caacagcgtt tcgctgcggg taagcgtggc atagttctca
    46981 acaatattgg cggtgttgac ggtcagttcg aagtgccact gtagcgcttc gttcagaggc
    47041 aacgttttgc cctcgacggt gacaggttca tcgcctttca gccacagcag ttcgacaagt
    47101 tctttcacca gtgccggatc gttctgatac cagacaccca gcgcgtcacc cggctggtaa
    47161 cgcaggcccg agtcacctaa gtcaatttcg atatggcgaa cgtctttttc agagttacgc
    47221 ccggtaattt tctggttaac cgaaaggctc gctaccagcg gcgcgtcttt gctgtacggg
    47281 ctggtgtgga tttcatttac cacgccagta gcgacggatt gcgaaggtgc cgcgacaggc
    47341 gcacgcgatt taagcgcatc aaccacgcgg gcgcgccact cgctggcagc agcctggtat
    47401 tcaacgtcgg cgtcgacacg gtcgagcagg cgttcaccgc ccagttccgc cagcttgctg
    47461 tcgaaatctt tcccggactg gcagaaaaat tcataagaac tatcgccgag gctaaacacg
    47521 gcaaaagcgg tattttccag cttcggcgct tttttggaga acaggaactt atgcagcgcg
    47581 acggcttctt ccggcggttc cccttctcct tgcgttgacg tcaccacgat aagcagtttt
    47641 tcgctggcga tttgtttgaa tttatagtcg cccgcgttca ccagcttaac gttcagtttt
    47701 gccgtcaata aatcatcgcg taatgcttca gcaacccggc gcgcattgcc ggtttgcgaa
    47761 gcagagataa tagttatacc cggcatttct gcggctggcg ctggcgtcgc tgcaagcgca
    47821 gcaggctgct gattgagcac gccccagaaa tagccagaaa cccaggcaag ctgggtggga
    47881 gttaaatcgg tcgtggccgc ctgaaggcgt gccagttgct ccgggttcaa cggaagcaac
    47941 gcggaaggtg ggacctgtgt cgtcatgcgt cgttatgttc cagtaagcaa agctgtttct
    48001 gcgccctgtc agcgcccata aaacagaaga gaaggtaagg ttaacggggt gaacggtgtg
    48061 gattaaagac gggatagcga taactaataa ccaaatcgac taacctgttt tagcaatagt
    48121 ctttaacaac aaaatagatt aaccaaccta atgaaaaaca aatgaattta gccaatcatt
    48181 aagataaatc agcgattttg cgcaacaaag tcgttttaga taatgcgaaa aaagagcctt
    48241 tccggtactc tacggcggtt ttatcgtcct gtagagaaat tatgatgtcc accacgttat
    48301 ttaaagattt caccttcgaa gccgctcacc gcttaccaca cgtcccggaa gggcataaat
    48361 gtggtcgcct gcacgggcat tcctttatgg tgcgactgga aattaccggg gaagtcgatc
    48421 cgcatacggg ctggattatc gatttcgctg aactaaaagc ggcgtttaag ccaacttatg
    48481 agcgcctcga tcaccattat ctcaatgata ttccaggtct ggaaaaccca actagcgaag
    48541 ttttagcaaa atggatttgg gatcaggtta aacccgttgt gccgctgtta agtgcggtga
    48601 tggtaaaaga aacctgcacc gctggttgta tctatcgcgg cgaatgataa gagtgtggcg
    48661 gcggtcaatt tcccttaagt aacgctatgt tagggtgttg tgttcaggat atctggggca
    48721 tgacatggaa gacgactgcg acattattat tattggtgcc ggtattgcag gcaccgcttg
    48781 cgcgttacgc tgcgcgcgag cgggtttatc cgttttgtta ctggaacgcg ctgaaatccc
    48841 cggcagcaaa aatctttccg gcgggcggtt atatacccat gcactcgcgg aactcctccc
    48901 tcaatttcat ctgaccgcgc ctcttgaacg acgcatcact cacgaaagcc tttccctgtt
    48961 aacgccggat tgcgcaacga cgttttccag cttacagccc ggcggtgaat cctggagtgt
    49021 attacgtgca cggttcgatc cgtggctggt tgccgaagcc gaaaaagaag gtgtcgaatg
    49081 catccctggt gcgacggtgg atgcactgta tgaagaaaac ggcagggtgt gtggtgtcat
    49141 ttgtggtgac gatattctcc gcgcccgtta tgtggtgctg gcagaaggtg ccaacagcgt
    49201 cctggctgaa cgtcacgggt tagtgactcg tcctgctggc gaagcgatgg cgttggggat
    49261 caaagaagtg ctgtcgctgg aaacatccgc tattgaagaa cgttttcatc tggagaataa
    49321 cgaaggcgca gcgttgctgt tcagcggcgg aatctgtgat gacttacccg gcggcgcatt
    49381 tctttatact aatcaacaaa cgctctcgtt agggattgtt tgcccactct cttcccttac
    49441 gcaaagtcgt gttccggcaa gcgagctgct tgctcgcttt aaaacgcatc cggcagtgcg
    49501 cccgcttatc aaaaacacgg aatcactgga gtatggtgcg catctggtgc cagaaggtgg
    49561 cttgcacagt atgccggtac aatacgccgg taacggctgg ctgctggtgg gcgatgcgtt
    49621 gcgcagttgc gtcaataccg gaatttctgt gcgcggcatg gatacggcgc taactggcgc
    49681 gcaggcggcg gcacaaacac tgataagcgc ctgccagcac cgcgagccgc aaaatctgtt
    49741 tccgctttat catcacaacg tagagcgcag cctgctgtgg gatgttctac agcgttatca
    49801 gcatgttccg gtgcttttgc aacgcccggg atggtaccgt acgtggcctg cgttaatgca
    49861 ggatatttcc cgcgatttat gggatcaggg tgataaacct gttccaccgc tgcgccagtt
    49921 actctggcgt catttacgtc gtcatggctt gtggaatctg gcgggcgatg ttatcaggag
    49981 tgttcgatgt ctgtagcccg taatctctgg cgcgctgctg atgcgccgca cattgttccg
    50041 gctgactccg ttgagcgtca gatggtacaa cggttgatta acgcctgtcc ggtaggtctt
    50101 ttttcgctca caccggaagg tgacttacgc gttgattatc acggctgcct ggagtgtggc
    50161 acctgccgtt tgctgtgcga cgaatccaca ctacaacagt ggcgctatcc gccttccgga
    50221 ttcggcatca cctaccgatt tggataaata ataaggataa tttatgcccc tcttacacct
    50281 gctccgccag aatccggtga ttgctgccgt taaagataat gccagcctgc aactggccat
    50341 cgattctgaa tgccagttta tttccgtctt gtacggcaat atctgcacca ttagtcaaat
    50401 cgttaaaaag attaagaacg ctgggaaata tgcttttatt catgttgatt tgctggaagg
    50461 cgcgtcaaat aaagaagtgg tgattcagtt tttgaaactg gtgaccgagg cggatggaat
    50521 tatcagcacc aaagcatcga tgctaaaagc ggcaagagcg gaaggttttt tctgtattca
    50581 tcgcctgttt attgttgatt cgatttcgtt tcacaacatt gataagcaag ttgcgcaatc
    50641 gaatccggat tgtattgaga tcctgccagg ctgcatgccc aaagtgctgg gctgggtgac
    50701 ggaaaaaatc cgccaaccgc tgattgctgg cgggctggta tgcgatgaag aagatgcgcg
    50761 taatgcgatt aacgcgggtg tcgtggcgct ttccaccacg aataccggag tctggacgtt
    50821 agcgaaaaaa ttgctttgac gggataatcg taaccaattg aatttggttt gatttgtagg
    50881 ccgcacgcca catccgacat tcagcgcctg atgcgacgct taacacgtct tatcaggcct
    50941 acaggtcccc agtccagaac cgtaggcagg atcatccggc agcacgttat tggcattctg
    51001 cgtcaatgtt tgtcaccagt gcctccagca tcactttcca gtcatcaacc acgccaacat
    51061 ctgcctgtga aaataccgct gcgctggcgt catgattaat cgccaccaca aacttgctat
    51121 tgcgtacgcc cgccattaac gctgctgcgc cagaagcgcc aacaacaata cacacctcag
    51181 gtgccagcag atgtccggaa ataccaatca ctttttcagc atcaacgcca ccgttcatca
    51241 ctcgcgcccg actgtagccc acttcggctc ccagctttta tgccagcata gcgatttcct
    51301 ggttatctgc ctctccgccc tgccccacca ccaacacccg tctggcctca gctaatgggt
    51361 cgcgggtaac atttttcagg ttctctatgc taaccagcca gtccggaagc gcacctggga
    51421 ctatgttcag ttgctgcata ccagagggca acgtggcatt tttatctgct cccgcctggc
    51481 gcgccaggga aagacatagc gggcgttttt ctgtttgcag cgtcgccgtt agcgcatttc
    51541 cccagtggga tttgcgcacg cttacgattg gaatctctaa cgaagtcacc tggcagatgc
    51601 ttgcaccatg taaacgccag gccagccgag tcgacagttc atcgccaaac gttcccggcg
    51661 gaaagagaac aacgttcgca ggcgttcgct gccactgttc caccagcgca tccagcacct
    51721 gttccaccac tacgggctgt ggttctattt gccagtgcgc cagggtacaa ccgctgaaat
    51781 cctgcgccgc cagccagccc gctaatgcgg cattctcctg atttattgtc acaattgcga
    51841 tattcatggc tgcatccttt ggcgtaaata gtcctgccat agcttttgcg ctttttctgc
    51901 taccgtttgc ccatcaatca gcgtcgctcc gcgtcgttgc tctgctcttg ccagttgcag
    51961 acactgcatc gctggcaatt cggcagcaac agttttacgg ataatttccg ctttccccgc
    52021 agccattcgc tggcgcattc ccggtactgg caaggccact tcaccgcact gacgcacggc
    52081 aatcaccgca ggcaagcgaa tccgacaaca gcgcagccca tgttcagtac gctgttcgag
    52141 ggtgataaac ggcgcgtcga gagtgaaacg ctccacctgg gtgaagcaag gccagcccag
    52201 catttccgcc agcaaaaatg gcgtttgccc attctgccct tcgctgcttt ggcagccggt
    52261 aattatcaga tccagcggat tctgacgctg ccattcggca atatggcgag cgacaaattc
    52321 cgacgcaaag cgcagatctg ccgccgtctc cagcaataca gcttcctcaa acccaagagc
    52381 catgagatag cgcagccagt gcaacgcccg ttcatccccc atgctcaacg cggttaaaga
    52441 catcggcgta ccgttttttc tctgcgccaa cagcagcgcg gcggcagcct gttcatcagc
    52501 accgagtaaa cttcgcagta gcgaaatatc cggtccgcta ttaccatgag ccgccgcctg
    52561 ccactctttt tccgccagca ttccggcatc cggttcggct ttaaacgcta acagaatgtt
    52621 cattcgcacc tcctacattt cccgccgcca ccagcgggag tgctttggtt tctggtgccc
    52681 atagccaggt tacaacaaaa ccagccaaca acactgccgc caggagcaat aaggtgactt
    52741 gcattcccca ctgcgccagc acccacggca gcaggccagt acttaccgcc gcccccaacc
    52801 gactcatggc ggtggcaaaa ccgacgccca gcgagcgaat gtcagtagga aaactttccg
    52861 caggcaaaat gcccaccaga ttactgactg ccgaaatggt ggtgctgaag agaacaaaag
    52921 cagcagcgtt aatgaactgc cggaaggcaa acaggccatc actaccagcg ttgccgccag
    52981 cagcaaaaaa cttcccagca aaaatttgcg atgtgccagc aggtgcgtca gaactaatcc
    53041 cagcagcgcg cccacaatta acaacgcatt aagcatcagg ctggcagtca gcgcatcttc
    53101 cagtccaata gtctgggcga tagttggcag ccaggtataa atcacaaacc atgggattac
    53161 gaggcagaca aagaagacgc tgttaaacgc cgtgcgccgc cagtagcgcg aagagaacaa
    53221 ggttttgatg tgtttatggg tcgccgttgc cacttcatcg cccagtaaaa catggggacc
    53281 aaaatagcga tgcacgatag cgtgagcttc tgcaaaacgc ccctggcgca gtagccagcg
    53341 tggcgattct ggcgttcccc agcgtaataa cgtaatcaac aacgcgggca gagctgccga
    53401 tgccagcagc cagcgccagg cctccgggtt ttcggaaata aagtgatgtc cggcaatact
    53461 tgccaacaca tagcctacgg tccacaccac gctgaatgcg cccagcaaaa caccgcgatg
    53521 gcggcgcggg gaaaattcag ccagcaaggt gtgacctact gagtaatcgc ctcccagacc
    53581 aatgccgatc aaaatgcgca gtccaataag atgctctggc gtggtggcaa aaaattgcaa
    53641 gaacgaagcg agcgtaatca gcaaaaagct gaaggtgaag attttttgcc gaccaatatg
    53701 gtcggagatc cacccaagaa ccaggctgcc aagaaacaaa ccaaggtgcg ccgagccgcc
    53761 gatcattccc gccataaacg gcgtcagttg catggcgggc gtaagttgaa taatggcgta
    53821 accaatgacg ccgagaacat aaccgtcggt caggtgtgcg ccgaaagtga gcgcagcaat
    53881 tcgacagtga aaacggttaa gcggtaaatc atccattcgc accggtgaag tgttcatctt
    53941 taccccttca ttgtccggta ataagaacgg cttctcgcct gagaagccga aaaggcaacg
    54001 ccccatcatc gcggaacgct gcctgacaca ttatttttca atcggataga tagtacccgt
    54061 attcataatg ccattaggat cgaactgctt tttcagccct tccagcaacg cccacgcgct
    54121 gccgtgttcc agcttgctcc agtgaacgcg atgtttaccg ataccgtggt ggtgcaccat
    54181 cgaaccaccg aggcgaatgg tttcttcgca aatgattttg ttgagcggat tgtggtactt
    54241 gtcgatttcc tcttccggct tacagttgac aacgttgtag tcgtagacga agtacatgtt
    54301 ggtgccgttc tgatagctat gagaggagtg accgcccagc atggtgatgt cgtcggcgtg
    54361 cgggaactcg gtacgaatac ggttaataac gctttcgtag atttcgtgga tgcagctcca
    54421 gcagccggac acttcggtag taaagcccat gttgccggtt ttgaggatct gcacacgttc
    54481 ggcagccact ttatccggtc cccagttcag gttgttaaac caggtttcga tcagcttgct
    54541 gtccacgcgt tggcattgcg ggtagcgggc aacgatttcc gcaatccctt cgcccgtcgc
    54601 cttcgcaatg cgagggttac cttcagccat aaagatcagc acgcattttc cgtcggcaaa
    54661 atgggtgaag tgttgggtgc catcttcagc gtcatacaaa cgagcgatcg acggacgata
    54721 cccttccacc atgatttcac gcaggatgtt gaagccggtt ttcatgtctt ccaggatata
    54781 gccgtagaag aggttgtttt ccggggtgaa tttaaagatt ttcactgtta cttcagtgat
    54841 atagcacaat gcaccttcgt tgccgatgat gatgtgacga atgtccgggc cagccgcgcg
    54901 gcgtggcacg tttttaatgc gtgtgacggt gccatctgcc aacactgcct ccagaccaac
    54961 gaccatatct tcgattgcgc cgtagagtgt ggagaactgc ccgatactgc gggttgctac
    55021 caggccgccc atctgcgcca gcggctttga ctgcggagaa tgccccgtgg tgtaaccttt
    55081 ttcacgcaac gcgttttcca gcacctccag cggaacacca cattgcgccg tcgcctgcat
    55141 attctcaata tcaatattaa tgatttgatt catggcggaa ccgtcgagca ccaccgagtt
    55201 ttctacaaca gtttccagcc caccttcggt ggcggaagca ccggtacgcg gcacaccgtt
    55261 aattttgtgc gcattcataa aattcagcac acgggatact tgctctgtgg aaccgagttt
    55321 tacgaccgct gccggaatcg gcaaagtata aatgccatga atatccggaa atttacgaaa
    55381 acggtcaata ctgttcttct ttaatactgt ttcatcggta attacgcgat ctgcaccaac
    55441 aatttccttt agctggtcga caatcgctgc gcgagataaa gacatagtaa ttccttcctg
    55501 ataataaaaa tggaataatc attaataaga gaaaacagaa accaccgtta tgggttagcg
    55561 cactaaataa ccgccatcga ccactaataa atgcccgttg acataattcg atgcctgact
    55621 tgcgagaaac acggctgcgc ccattaaatc ctgagtatcg ccccaacggt ttgccggaat
    55681 atgatcaaga acgcgctgat tggtttctgg attactgcgt gtcgccagcg taatgtctgt
    55741 tgcataatag ccaggggcga taccatttac ctgaatatta tattgaccta gttcatcaca
    55801 ataagctttg gtgaatccgg caagagcatg tttagtagca gaatatgcag gtgaccattg
    55861 tccacctaag taagagaaca atgaacagat attaatgatt ttaccgcttt tctgcgggat
    55921 cataattttt gccgcttcgt agcttaactc gaatgcggcg gtcaggttca catcaatcat
    55981 cgggtcccag tcggcacgac cgaagtccag caccttattc agcttacaaa taccggcatt
    56041 gttaaccaga atatcaactg taccgaaacg ctcacagcag gcagcgataa tcttctgcgg
    56101 cgcgccttct gcggtgatat ccacctgcat gaagtccacc tcaacaccct gattttcaat
    56161 catttccttt gtttcgccgt tatctttgac aaaacttgga ataaagacat ttgcgcctgc
    56221 tttggccaac gccatggcaa atgcctggcc taaaccgcta ttcccgccgg taacaattgc
    56281 ggttttacct ttcagggaga aaaaatccat tgagaacgca ttgagagatt cgattgacat
    56341 agttagctcc aatttccgta aggcaaaaaa aaagaaaggt aacttccccc gcgagaggga
    56401 agttaccttt ctcattatct ctggtaactg gagataattt ttagcataag gacataactg
    56461 ctaatgtgat cgtaatcaca gtgtgatatt cgtattatca aatagtttta aaaagcagaa
    56521 gcgtgattac tctcacacaa cacctctgct ttccatgtta tttattattt caattactag
    56581 agacctgaga aaagtaatcc acctgaaagg gcggcttact tttctctttt ttttgtctat
    56641 tacaaatggg aatacgatat gcaacacaac tcatatcgcc gttggataac cctcgcgata
    56701 attagtttta gcggcggcgt tagtttcgac ctggcttatt tacgttatat ttatcaaatt
    56761 cccatggcga aatttatggg attcagcaat accgagatag gtttaataat gagtaccgtt
    56821 ggtattgcgg ctattattct ttatgccccc agcggcgtta ttgccgataa attttcacac
    56881 cgcaaaatga ttacttccgc gatgatcatt accggattac tgggtctgtt aatggcaacg
    56941 tatccaccgc tgtgggtaat gctctgtatt caggtcgcct ttgcgataac aacgatttta
    57001 atgctgtggt cggtgtcgat taaagccgca tcgttgcttg gcgatcatag cgagcaaggg
    57061 aaaattatgg gctggatgga agggctgcgc ggcgtcggtg taatgtcgct ggcagtgttt
    57121 accatgtggg ttttttctcg ctttgcaccg gatgacagca ccagcctgaa aacggtcatt
    57181 atcatctata gtgtggttta catcttgttg gggattctgt gctggttttt tgttagcgat
    57241 aacaacaacc tgcgcagtgc caataacgaa gaaaaacagt cattccagct tagcgacatc
    57301 cttgccgttt tgcgtatcag caccacctgg tattgcagca tggtgatttt tggcgtcttc
    57361 accatctacg ccattctgag ttactccacc aactatctga ccgaaatgta tggcatgtcg
    57421 ctggtggcgg cgagctacat ggggattgtg atcaacaaaa tcttccgcgc gctgtgcggc
    57481 ccacttggcg gcattatcac cacctacagt aaagtgaaat cccctacccg cgtgatccaa
    57541 atcctttccg tactcggcct gctggcgtta actgccctgc tcgtcacgaa ctctaacccg
    57601 caatcggtcg cgatggggat tggcctgatt ttactgctgg gattcacctg ttacgcctca
    57661 cgcgggctgt actgggcctg ccctggcgaa gcgagaacac cgtcttacat tatgggcacc
    57721 acggtaggta tttgctcggt gattggattc ctgccggatg tcttcgttta cccaattatc
    57781 ggccactggc aagacaccca gcccgcagca gaagcctacc gcaatatgtg gctgatgggc
    57841 atggcggcgc ttgccatggt gattgtcttt acctttttgc tgttccaaaa aattcgtact
    57901 gctgatagcg cccccgcaat ggccagcagc aagtaagcca ggaaaattac aggagaaaaa
    57961 tcatgtcgaa gaaatacatc atagggattg atggcggaag tcagagcaca aaagtggtga
    58021 tgtacgatct ggaaggtaac gtggtttgcg aaggcaaagg tttattgcag ccgatgcaca
    58081 cgccagatgc cgataccgca gaacatcctg acgacgattt atgggcatca ttatgttttg
    58141 ccggtcacga tttgatgagt cagtttgccg ggaataaaga agatattgtc ggtattggtc
    58201 tgggatccat ccgttgctgc cgtgcgttat tgaaagccga tggcacgcct gctgcgccgt
    58261 tgattagctg gcaggatgca cgcgtaacgc gtccctacga acacactaat cctgacgtgg
    58321 cctatgtcac ctctttttcg ggttatctga cgcatcgctt aaccggcgag tttaaagaca
    58381 atatcgccaa ctattttggt cagtggccgg tggattataa gagctgggca tggagcgaag
    58441 atgctgcggt aatggagaag tttaatatcc cccgtcatat gctgtttgat gtgcaaatgc
    58501 caggcactgt tcttggacag atcacaccac aagccgcgct ggcgacacat ttcccggcag
    58561 ggctgccggt tgtttgtacc accagtgata aaccggtaga agctctggga gccggattac
    58621 tggatgatga aacggcggtg atttcactcg gtacttatat cgcgctgatg atgaacggca
    58681 aagccttgcc gaaagatccg gtggcgtact ggccgattat gtcttctatt ccgcaaacat
    58741 tgctgtatga aggttacggt attcgcaaag gcatgtggac ggtgagctgg ctgcgcgaca
    58801 tgttaggcga gtcgttaatt caggatgcca gggcgcagga tctttcaccg gaagatttac
    58861 tcaacaaaaa agcttcttgt gtgccacctg gctgtaatgg gctgatgacg gtgctggact
    58921 ggctgaccaa tccgtgggaa ccgtacaaac gcgggattat gatcggcttt gattccagca
    58981 tggattacgc atggatatat cgttcgatac tggaaagcgt ggcgctgacg ctgaagaaca
    59041 attacgacaa tatgtgtaat gaaatgaatc actttgcgaa gcatgtgatc attactggtg
    59101 gcggttcgaa cagcgatctg tttatgcaga tttttgccga cgtgttcaac cttccggcac
    59161 gacgtaacgc cattaacggt tgtgcaagtc tgggggcagc gatcaatacg gcggtaggtc
    59221 tggggctata cccggattac gcaacggctg tcgacaaaat ggttcgcgtg aaagatatct
    59281 ttataccgat tgagagcaat gccaaacgct acgacgcgat gaataaaggt attttcaaag
    59341 ccctaaccaa acatactgat gtgatcctga aaaaatcgta tgaagtgatg catggggaat
    59401 tggggaatgc ggattcgatc cagagctggt cgaatgcgta agtgaggaag accgggcggg
    59461 aaactgcccg gcctgaacat acctgaatgg ttatccccgc tggcgcgggg aacataagtt
    59521 cgacaactta aaaaatgata acaagtccgg tttatccccg ctgatgcagg gaacataata
    59581 aaaactttaa ctagttcggt ttatccctgt ttttacaagg aatattaact acactcgatg
    59641 tctttagaat aaaagacaat acgaatcgca ctttatcaat tttcacttaa agtatgaact
    59701 ggatatcttt attcataaga actattcatc aacagcgtat agaggcggtt atgtaaaacc
    59761 actcattagc ctc
//
LOCUS       DAZFAB010000019        57651 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZFAB010000019 DAZFAB010000000
VERSION     DAZFAB010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57651
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.019"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..818
                     /locus_tag="ACHJWA_001354"
     CDS             <1..818
                     /locus_tag="ACHJWA_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YPO3983 family protein"
                     /protein_id="HFO4307992.1"
                     /translation="PCTLFETISLFDNYSADDMQYGDMVEQDFLSLGLSDISAKVDPY
                     RLIKYHFPGPGSINVAFSASSSGTKISQRECTDILFAEMKELAKMFSFFGQYKTLIED
                     LIEHFRYGNGSNFHSQQLNLSFHEKINKYGYNSPIRIIKECIENGINSTPSTGYQPLI
                     LQSIKTKLLSSRLNKFNDFEDSFNGLGISVHDISAQKISLLSFQKYAIGWSATIHFVA
                     QDHFGLDVTDIKNKTYSKYRFFRIWFFLQRHKDFAFKPFFTNFNTIERIENYL"
     gene            815..1306
                     /locus_tag="ACHJWA_001355"
     CDS             815..1306
                     /locus_tag="ACHJWA_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HFO4307993.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCITKNYLMVINEYPYRNTHIGINDFTEYQLTQENKIEQVFNPHNFKK
                     DNF"
     gene            complement(1348..2298)
                     /gene="corA"
                     /locus_tag="ACHJWA_001356"
     CDS             complement(1348..2298)
                     /gene="corA"
                     /locus_tag="ACHJWA_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFO4307994.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(2450..2506)
                     /gene="ysgD"
                     /locus_tag="ACHJWA_001357"
     CDS             complement(2450..2506)
                     /gene="ysgD"
                     /locus_tag="ACHJWA_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFO4307995.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            2668..3432
                     /gene="yigE"
                     /locus_tag="ACHJWA_001358"
     CDS             2668..3432
                     /gene="yigE"
                     /locus_tag="ACHJWA_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFO4307996.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     MLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            3515..4417
                     /locus_tag="ACHJWA_001359"
     CDS             3515..4417
                     /locus_tag="ACHJWA_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HFO4307997.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSIV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVREIDEKANRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSRQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCDK"
     gene            complement(4462..6624)
                     /gene="uvrD"
                     /locus_tag="ACHJWA_001360"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(4462..6624)
                     /gene="uvrD"
                     /locus_tag="ACHJWA_001360"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFO4307998.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(6708..7424)
                     /gene="yigB"
                     /locus_tag="ACHJWA_001361"
     CDS             complement(6708..7424)
                     /gene="yigB"
                     /locus_tag="ACHJWA_001361"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFO4307999.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEQEALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(7424..8320)
                     /gene="xerC"
                     /locus_tag="ACHJWA_001362"
     CDS             complement(7424..8320)
                     /gene="xerC"
                     /locus_tag="ACHJWA_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFO4308000.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQNWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKTPRHLPKNIDVDDINRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNALSWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(8317..9024)
                     /gene="yigA"
                     /locus_tag="ACHJWA_001363"
     CDS             complement(8317..9024)
                     /gene="yigA"
                     /locus_tag="ACHJWA_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HFO4308001.1"
                     /translation="MKQPGEELQETLTELDDRVVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(9021..9845)
                     /gene="dapF"
                     /locus_tag="ACHJWA_001364"
     CDS             complement(9021..9845)
                     /gene="dapF"
                     /locus_tag="ACHJWA_001364"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFO4308002.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(9882..10085)
                     /gene="yifL"
                     /locus_tag="ACHJWA_001365"
     CDS             complement(9882..10085)
                     /gene="yifL"
                     /locus_tag="ACHJWA_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO4308003.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            10244..10386
                     /locus_tag="ACHJWA_001366"
                     /pseudo
     CDS             10244..10386
                     /locus_tag="ACHJWA_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            10548..10868
                     /gene="cyaY"
                     /locus_tag="ACHJWA_001367"
     CDS             10548..10868
                     /gene="cyaY"
                     /locus_tag="ACHJWA_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFO4308004.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFRDLLEQAATQQAGET
                     VSFR"
     gene            complement(10908..13454)
                     /gene="cyaA"
                     /locus_tag="ACHJWA_001368"
     CDS             complement(10908..13454)
                     /gene="cyaA"
                     /locus_tag="ACHJWA_001368"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFO4308005.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            13841..14782
                     /gene="hemC"
                     /locus_tag="ACHJWA_001369"
     CDS             13841..14782
                     /gene="hemC"
                     /locus_tag="ACHJWA_001369"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFO4308006.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            14779..15519
                     /gene="hemD"
                     /locus_tag="ACHJWA_001370"
     CDS             14779..15519
                     /gene="hemD"
                     /locus_tag="ACHJWA_001370"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFO4308007.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPNYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            15541..16746
                     /gene="hemX"
                     /locus_tag="ACHJWA_001371"
     CDS             15541..16746
                     /gene="hemX"
                     /locus_tag="ACHJWA_001371"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFO4308008.1"
                     /translation="MTEQEKTSAVVEETKEAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKVELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQQETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPQAD
                     TPAAAPQGE"
     gene            16749..17945
                     /gene="hemY"
                     /locus_tag="ACHJWA_001372"
     CDS             16749..17945
                     /gene="hemY"
                     /locus_tag="ACHJWA_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFO4308009.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            18624..20279
                     /gene="aslA"
                     /locus_tag="ACHJWA_001373"
     CDS             18624..20279
                     /gene="aslA"
                     /locus_tag="ACHJWA_001373"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase AslA"
                     /protein_id="HFO4308010.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYLVKPATTIADNMMPVMQHPAQDKEPQQKLAELEKKTGKKPNVVVFLLDDVGWMDVG
                     FNGGGVAVGNPTPDIDAVASQGLILTSAYSQPSSSPTRATILTGQYSIHHGILMPPMY
                     GQPGGLQGLTTLPQLLHDQGYVTQAIGKWHMGENKESQPQNVGFDDFRGFNSVSDMYT
                     EWRDVHVNPEVALSPDRSEYIKQLPFSKDDVHAVRGGEQQAIADITPKYMEDLDQRWM
                     EYGVKFLDKMAKSDKPFFLYYGTRGCHFDNYPNAKYAGSSPARTSYGDCMVEMNDVFA
                     NLYKALEKNGQLDNTLIVFTSDNGPEAEVPPHGRTPFRGAKGSTWEGGVRVPTFVYWK
                     GMIQPRKSDGIVDLADLFPTALDLAGHPGAKVANLVPKTTFIDGVDQTSFFLGTNGQS
                     NRKAEHYFLNGKLAAVRMDEFKYHVLIQQPYAYTQSGYQGGFTGTVMQTAGSSVFNLY
                     TDPQESDSIGVRHIPMGVPLQTEMHAYMEILKKYPPRAQIKSD"
     gene            complement(20447..21682)
                     /gene="aslB"
                     /locus_tag="ACHJWA_001374"
     CDS             complement(20447..21682)
                     /gene="aslB"
                     /locus_tag="ACHJWA_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO4308011.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETTAREGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVSK"
     gene            complement(21829..21905)
                     /locus_tag="ACHJWA_001375"
     tRNA            complement(21829..21905)
                     /locus_tag="ACHJWA_001375"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21869..21871),aa:Pro,seq:tgg)
     gene            complement(21948..22034)
                     /locus_tag="ACHJWA_001376"
     tRNA            complement(21948..22034)
                     /locus_tag="ACHJWA_001376"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21998..22000),aa:Leu,seq:cag)
     gene            complement(22055..22130)
                     /locus_tag="ACHJWA_001377"
     tRNA            complement(22055..22130)
                     /locus_tag="ACHJWA_001377"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22095..22097),aa:His,seq:gtg)
     gene            complement(22189..22265)
                     /locus_tag="ACHJWA_001378"
     tRNA            complement(22189..22265)
                     /locus_tag="ACHJWA_001378"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22229..22231),aa:Arg,seq:ccg)
     gene            complement(22368..23753)
                     /gene="yifK"
                     /locus_tag="ACHJWA_001379"
     CDS             complement(22368..23753)
                     /gene="yifK"
                     /locus_tag="ACHJWA_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO4308012.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(23944..24684)
                     /gene="wecG"
                     /locus_tag="ACHJWA_001380"
     CDS             complement(23944..24684)
                     /gene="wecG"
                     /locus_tag="ACHJWA_001380"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFO4308013.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEENAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTTVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(24687..26039)
                     /gene="wzyE"
                     /locus_tag="ACHJWA_001381"
     CDS             complement(24687..26039)
                     /gene="wzyE"
                     /locus_tag="ACHJWA_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFO4308014.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(26036..27115)
                     /gene="rffT"
                     /locus_tag="ACHJWA_001382"
                     /gene_synonym="wecF"
     CDS             complement(26036..27115)
                     /gene="rffT"
                     /locus_tag="ACHJWA_001382"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFO4308015.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFYWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDASLNTMANDRQREGKMTILVGNSGDRSNEHIAALRAVHQQFGDTVKVVVP
                     MGYPPHNEAYIEEVRQAGLELFSEENLQVLSEKLEFDAYLTLLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(27112..28362)
                     /gene="wzxE"
                     /locus_tag="ACHJWA_001383"
     CDS             complement(27112..28362)
                     /gene="wzxE"
                     /locus_tag="ACHJWA_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFO4308016.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISLGLFGNHDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAVMLIKRGAIPLSYLKPSWDNGLAGQLS
                     KFTLMTLITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPTHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(28364..29494)
                     /gene="rffA"
                     /locus_tag="ACHJWA_001384"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(28364..29494)
                     /gene="rffA"
                     /locus_tag="ACHJWA_001384"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFO4308017.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCLQNAHMFYIKLRDIGDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(29499..30173)
                     /gene="rffC"
                     /locus_tag="ACHJWA_001385"
                     /gene_synonym="wecD"
     CDS             complement(29499..30173)
                     /gene="rffC"
                     /locus_tag="ACHJWA_001385"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFO4308018.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPFLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNASDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASSRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(30151..31032)
                     /gene="rfbA"
                     /locus_tag="ACHJWA_001386"
     CDS             complement(30151..31032)
                     /gene="rfbA"
                     /locus_tag="ACHJWA_001386"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFO4308019.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEVATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(31051..32118)
                     /gene="rffG"
                     /locus_tag="ACHJWA_001387"
     CDS             complement(31051..32118)
                     /gene="rffG"
                     /locus_tag="ACHJWA_001387"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFO4308020.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNALTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPQGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWRKQVQDGSYQGERLGLKG"
     gene            complement(32118..33380)
                     /gene="wecC"
                     /locus_tag="ACHJWA_001388"
     CDS             complement(32118..33380)
                     /gene="wecC"
                     /locus_tag="ACHJWA_001388"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFO4308021.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(33377..34507)
                     /gene="wecB"
                     /locus_tag="ACHJWA_001389"
     CDS             complement(33377..34507)
                     /gene="wecB"
                     /locus_tag="ACHJWA_001389"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFO4308022.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(34563..35609)
                     /gene="wzzE"
                     /locus_tag="ACHJWA_001390"
     CDS             complement(34563..35609)
                     /gene="wzzE"
                     /locus_tag="ACHJWA_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFO4308023.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(35621..36724)
                     /gene="wecA"
                     /locus_tag="ACHJWA_001391"
     CDS             complement(35621..36724)
                     /gene="wecA"
                     /locus_tag="ACHJWA_001391"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFO4308024.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGNLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFLLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(36964..38223)
                     /gene="rho"
                     /locus_tag="ACHJWA_001392"
     CDS             complement(36964..38223)
                     /gene="rho"
                     /locus_tag="ACHJWA_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFO4308025.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(38308..38409)
                     /gene="rhoL"
                     /locus_tag="ACHJWA_001393"
     CDS             complement(38308..38409)
                     /gene="rhoL"
                     /locus_tag="ACHJWA_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFO4308026.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(38550..38879)
                     /gene="trxA"
                     /locus_tag="ACHJWA_001394"
     CDS             complement(38550..38879)
                     /gene="trxA"
                     /locus_tag="ACHJWA_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFO4308027.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            39010..40275
                     /gene="rhlB"
                     /locus_tag="ACHJWA_001395"
     CDS             39010..40275
                     /gene="rhlB"
                     /locus_tag="ACHJWA_001395"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFO4308028.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHCLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPVNQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            40411..41895
                     /gene="gppA"
                     /locus_tag="ACHJWA_001396"
     CDS             40411..41895
                     /gene="gppA"
                     /locus_tag="ACHJWA_001396"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFO4308029.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(41942..43963)
                     /gene="rep"
                     /locus_tag="ACHJWA_001397"
     CDS             complement(41942..43963)
                     /gene="rep"
                     /locus_tag="ACHJWA_001397"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFO4308030.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            44180..44629
                     /locus_tag="ACHJWA_001398"
     CDS             44180..44629
                     /locus_tag="ACHJWA_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFO4308031.1"
                     /translation="MAINFSPKVGEILECNFGNYPVSQNGPFSTTYYDGRIPPEMIKN
                     RLVVVLNGKINGNAFIVVPLSTTRDHDKLKRGMHVEIASNVINDLQFFDQQIRWAKTD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            44828..45109
                     /gene="ppiC"
                     /locus_tag="ACHJWA_001399"
     CDS             44828..45109
                     /gene="ppiC"
                     /locus_tag="ACHJWA_001399"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFO4308032.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(45155..46630)
                     /gene="ilvC"
                     /locus_tag="ACHJWA_001400"
     CDS             complement(45155..46630)
                     /gene="ilvC"
                     /locus_tag="ACHJWA_001400"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFO4308033.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVHTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDLNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            46780..47673
                     /gene="ilvY"
                     /locus_tag="ACHJWA_001401"
     CDS             46780..47673
                     /gene="ilvY"
                     /locus_tag="ACHJWA_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFO4308034.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(47725..49269)
                     /gene="ilvA"
                     /locus_tag="ACHJWA_001402"
     CDS             complement(47725..49269)
                     /gene="ilvA"
                     /locus_tag="ACHJWA_001402"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFO4308035.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(49272..51122)
                     /gene="ilvD"
                     /locus_tag="ACHJWA_001403"
     CDS             complement(49272..51122)
                     /gene="ilvD"
                     /locus_tag="ACHJWA_001403"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFO4308036.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHSDRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDTLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQISFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(51187..52116)
                     /gene="ilvE"
                     /locus_tag="ACHJWA_001404"
     CDS             complement(51187..52116)
                     /gene="ilvE"
                     /locus_tag="ACHJWA_001404"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFO4308037.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(52136..52399)
                     /gene="ilvM"
                     /locus_tag="ACHJWA_001405"
     CDS             complement(52136..52399)
                     /gene="ilvM"
                     /locus_tag="ACHJWA_001405"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFO4308038.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(52396..54042)
                     /gene="ilvG"
                     /locus_tag="ACHJWA_001406"
     CDS             complement(52396..54042)
                     /gene="ilvG"
                     /locus_tag="ACHJWA_001406"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFO4308039.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLKLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(54045..54095)
                     /gene="ilvX"
                     /locus_tag="ACHJWA_001407"
     CDS             complement(54045..54095)
                     /gene="ilvX"
                     /locus_tag="ACHJWA_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFO4308040.1"
                     /translation="MNNSTKFCFPRFRTGN"
     gene            complement(54182..54280)
                     /gene="ilvL"
                     /locus_tag="ACHJWA_001408"
     CDS             complement(54182..54280)
                     /gene="ilvL"
                     /locus_tag="ACHJWA_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFO4308041.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            54633..56153
                     /gene="yifB"
                     /locus_tag="ACHJWA_001409"
     CDS             54633..56153
                     /gene="yifB"
                     /locus_tag="ACHJWA_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFO4308042.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLVADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSTTVKQRVMAARERQFKRQNKLNAWLDSPEIRKFCKLESEDAQWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(56178..56516)
                     /gene="maoP"
                     /locus_tag="ACHJWA_001410"
     CDS             complement(56178..56516)
                     /gene="maoP"
                     /locus_tag="ACHJWA_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFO4308043.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            56635..57474
                     /gene="hdfR"
                     /locus_tag="ACHJWA_001411"
     CDS             56635..57474
                     /gene="hdfR"
                     /locus_tag="ACHJWA_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFO4308044.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNTLIRDLLKINVLD
                     EVY"
     gene            complement(57570..57645)
                     /locus_tag="ACHJWA_001412"
     tRNA            complement(57570..57645)
                     /locus_tag="ACHJWA_001412"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(57610..57612),aa:Trp,seq:cca)
BASE COUNT        14241 a        15470 c        14553 g        13387 t
ORIGIN      
        1 ttccctgtac acttttcgaa actattagtt tatttgataa ttattctgct gatgatatgc
       61 aatatggtga tatggtagaa caagattttc tctcactcgg cctaagcgat atctcagcaa
      121 aagttgatcc ttacagactt attaaatacc atttccctgg cccaggttct attaatgttg
      181 cttttagtgc ctcaagttcc ggaacaaaaa tatcacaacg tgaatgcact gatattttat
      241 ttgcagaaat gaaagaacta gcaaaaatgt tttccttttt tggacaatat aaaacactta
      301 tcgaagatct gattgaacat tttagatatg gaaatggcag caattttcat agccaacaat
      361 taaatttatc ttttcatgaa aaaataaata aatatggtta taatagtcca atacgaataa
      421 tcaaagaatg tatcgaaaat ggcattaatt caactccctc gacaggatac caacctctta
      481 tcctgcaaag cattaaaacc aaattacttt catcaagact aaataaattt aatgacttcg
      541 aggacagttt taatggctta ggaatcagtg ttcatgatat ttcagctcaa aaaatatctc
      601 ttttaagttt tcaaaaatac gctataggat ggagtgcaac aattcatttt gtagcacagg
      661 accacttcgg tcttgatgta actgatatca aaaacaagac ttacagcaaa tatagatttt
      721 ttcgcatatg gttcttttta caacggcata aagactttgc tttcaaacct tttttcacca
      781 attttaacac tatcgaaaga atcgaaaatt atctatgaaa agcaaactcc aaaaaattat
      841 attatgttta tttcttctat gctgcatcta taatttatgg acattgcgtc ctgtacagat
      901 tttgtataca tactctgatg ctggcaattc agtctttctg gtcgttgatc atctgccatg
      961 gactgacagc gataaaataa attggtatct gaagcatcag aatgaaatta aaaatcaaca
     1021 tcccctacca gaaggtagtt ggcatacctg gtatgtcatt gatatcggta atggcttcac
     1081 tgactataaa aaatatatcg aagggccgta tgaagatctc tactgtttcc cgacaattaa
     1141 gagcaatgat aattgcatca cgaaaaatta cttaatggtt attaatgaat acccttatcg
     1201 taacactcat attggtatta acgattttac tgagtatcaa ttaacacaag aaaataaaat
     1261 agagcaagtt ttcaatccgc ataactttaa aaaagacaat ttttagaaat aagccggata
     1321 aaaaaatagc tttatccggc actcatatta caaccagttc ttccgcttaa agtacagata
     1381 cggtgccagg cccgcgagga tcataaagat aatcgcgcct gggtagccga aactccactt
     1441 cagttctggc ataaactcaa agttcatccc gtagctggaa gcaaccagcg ttggcggcag
     1501 gaacacgacg gataccaccg agaaaatttt gatgatgcgg ttctgctcga tgttgataaa
     1561 acccattgcc gcctgcatca ggaagttcac cttctggaac agggattcgt tatgcggcag
     1621 cagggattcg atatctcgca ggatttcacg cgcctgctcc agttgcccac ccggtaagcg
     1681 tgctttacgt accaggaagt tgagcgcgcg ctgggtatcc atcagacaca ggcgcacttt
     1741 ccagccgata tcttccagtt ccgccagagt ggagagcgcc tcgtcgtact catcgccctg
     1801 atgcccttcc ataatcaccc ggctcaattg ctccaggtcg ctatagatat tttcaatttc
     1861 atctgccaac tgttcgattt tggtttcgaa cagatccagc agcaactcgt aggcgttacc
     1921 gtctaccatc gactggctac gggcacgcat acgatacaga cgaaaagcgg gcagttcacg
     1981 ctcacgcaga gtaaacagac gaccatcacg gatggtaaat gccacagtgg agttaccggc
     2041 gtgatcttcc gcatcttcaa agaagaagaa ggagtgaata tgcaggccgt catcgtcttc
     2101 aaagaaacgt gccgatgctt cgatgtcttc cagttccggg cgggttgcca ggctctggcc
     2161 aagttcagat tgtacgcgca gtcgctcgtc gtcgtccggt tcgacaagat caatccatac
     2221 tgcatttaca aggggttgtg actcttcgac ttccagccgg gtcagtcggt tattttccag
     2281 ttgaaatgcg ctcagcatga ccgggactcc caatgcttaa aatatcggac agttcggtgg
     2341 gcacacagaa acaaattggg tttcagacca ttaaacagcc tgactcagcg cgacgggaaa
     2401 aattgaggtc gctgacaacc gctaaggcta tcagcaaaaa gggatagcct taggagttga
     2461 tcctggatga caggatagtg agccagtatc tactgggtgt gtccaaggcg aatgtcctct
     2521 tagagtgatc gtgcgcgcat gttacgccag caaaattttg ccgtcaacac gcaacagaac
     2581 accacagaac aatagttgaa cttcgcgctt tttggtactc gtcaaggtcg ctaacaggat
     2641 aaaaaaatcc cacatccagg acctataatg gcgcatcggc tactcattgg taaaggaatg
     2701 atcaccttga atctcaaacg gatttttctc gccctcacct tacttccctt gtttgctgtt
     2761 gccgctgatg attgcgcact ctcagatcca acgctgaccg tacaggcgta taccgttaat
     2821 cctcaaacag agcgggtgaa aatgtactgg caaaaagcca atggcgaagc gtggggaact
     2881 ttacatgctc tgctggcgga tatgaatagt cagggtcagg tgcagatggc gatgaacggc
     2941 ggcatctatg atgaaagcta tgcgccgctc ggtttgtaca tcgaaaacgg tcagcagaag
     3001 gtggcgttaa atctcgcttc aggtgaaggg aatttcttta tccgtcctgg cggcgtgttt
     3061 tatgtcgcgg gagataaagt cggcatcgtt cgtctggatg ccttcaaaac cagtaaagag
     3121 attcagtttg cggtgcagtc agggccaatg ttgatggaaa acagtgtaat taatccgcgt
     3181 attcatccca acgtcgcctc acgtaaaatt cgtaacggtg ttggaattaa taaacatggg
     3241 aatgccgtgt ttttgttgag ccagcaggcg acaaattttt atgattttgc ctgttatgcc
     3301 aaagcgaaac tgaacgttga gcagctgctt tatcttgatg gcacgatttc acatatgtat
     3361 atgaaaggcg gggcaatccc gtggcaacgc tatccttttg tcaccatgat ttccgtcgag
     3421 cggaaaggtt aagcattgta cactcgagga ggccagacaa actggggctg gctacacagc
     3481 tggaaaatca tcagataaat gaagggaaac aaaaatgaaa aaaacgctcg caacgttatt
     3541 tttattaacg tgtctgggat catcatccgc ttatgcggat aacgctttga ttttacaaac
     3601 tgattttagc ctgaaagatg gtgccgtctc ggcgatgaaa ggggtcgcat tcggtgttga
     3661 ccataatctt aaaatatttg atttaacaca cgaaattcca ccgtataaca tctgggaagg
     3721 cgcttaccgc ctgtatcaga ccgccagtta ctggccgcaa ggttcggtat ttgtcagtgt
     3781 ggtcgatccg ggcgtaggca ctgaccgtaa atcgatagtg ctgaaaacca aaaacggtca
     3841 atacttcgtt tcaccggata acggtacgct cactttggtg gcggaatcgc tgggtattga
     3901 gagcgtccgg gaaatcgacg aaaaagctaa ccgcctgaaa ggttctgaaa agtcatatac
     3961 cttccacggt cgtgacgtgt atgcctacac cggtgcacgt ctggcttccg gggcgatcac
     4021 gttcgagcag gtggggcctg agcttcctgc aaaggttgtc gaactttctt atcagaaagc
     4081 aaaagcaaca aaaggtgaag tgaagggcaa tatcccgatt ctcgatattc agtacggcaa
     4141 cgtctggagc aatatcagcg acgagttgct gaatcaggcg gggatcaaac tcaacgacac
     4201 gttgtgtgtg accatttccg aaggttcccg gcaaaaatac gcagggaaaa tgccgtatgt
     4261 cgcaagcttc ggcgatgtgc cggaaggaca gccgatggtg tatctgaaca gcttattgaa
     4321 tgtttccgtg gcgctgaata tggataattt cgcgcagaaa catcaggttg cgtctggtgc
     4381 tgactggaat attgatgtga agaaatgtga taagtaaggt gctgatgccg gatgcggtgt
     4441 ggcgcaccgc atcccgcaac gttataccgt ctccagccgg gcgtatgccg ccaccagcca
     4501 tttaataccc tggccctgga atgccacctg caaacggcta tgctcgccgc tgccttccat
     4561 attgacgata gtgccttcac caaacttagc gtggcgtaca cgctggccga gcttgtagcc
     4621 gctgtcgttc tcgaccatcg gcgtacccat acgctgatgg ctgaccgggc ggcttaccgt
     4681 ggcgcgcagg cgcacctctt ccacacactc ttccggcaac tcaccgataa agcgcgacgg
     4741 gcgatggtaa acctctttgc catacagacg gcgagtttcc gcgtaggtca gcgtcagttt
     4801 ctgcatcgcg cgggttacac caacgtaggc cagacggcgt tcttcttcca gacgtccgcc
     4861 ttcatccagc gacatctggc ttgggaacat gccctcttcc ataccgacga taaacacctg
     4921 cgggaactcc aggcctttcg ccgagtgcag cgtcatcaac tgcactgcat cctgccaggt
     4981 atccgcctgc ccctcgcccg cttccagcgc cgcatgggag aggaacgcct gcagcggcat
     5041 taaatcttcg tcttcttcgt tgtagctgaa ttggcgcgtt gccgtcacta gttcctctaa
     5101 gttttcgata cgcgtctgac ctttttcgcc cttctcctgc tcgtacatgg tacgcaggcc
     5161 ggagtcttta attacccggt cagtctgtac atgcagcggc atatcggcag tttcctgcgc
     5221 taaggcgtcg atcagttcca taaaccgctg taaggcgctg gcagcacgtc cggcgagggc
     5281 tttttcctgc aacagttcac gacatgcctg ccagagtgtt aactggcgat cgcgcgatgt
     5341 ctgacgtacc acgtccagcg tccggtcacc aataccccgc gttggcgtat tcaccacacg
     5401 ctcaaaggcc gcgtcgtcgt tgcggttggc aatcaggcgc agatacgaga gcgcatcttt
     5461 gatttcctgg cgttcgaaga agcgcatccc gccgtaaata cggtacggca tactggcctg
     5521 caataacgcc tcttcgagca cccgcgactg ggcgttgctg cggtagagaa tggcgcactc
     5581 ggcgagcgcc ccaccgttgt cctgccaggt tttgatgcgg ttaacgacaa aacgcgcttc
     5641 atcgagttcg ttaaaagcgc aatagaggga aataggctca ccgtccgcgc catcggtcca
     5701 cagtttttta cccagacgcc cgttgttgtt ttcaatcagg gcgttcgcgg cgctcagaat
     5761 gttactggta gaacgatagt tctgctccag acggatagtt tcggcaccgg ggaaatcatt
     5821 gaggaaacgc tgaatattct ccacctgcgc cccgcgccag ccgtagattg actggtcgtc
     5881 atcaccgacg atcatcactt tgccggtgtc gcccgccagc aggcggatcc acgcgtactg
     5941 aatgttgttg gtatcctgga attcgtccac caggatgttg gtaaaacgtt cgcggtagtg
     6001 ttgcaggata tgcggcttgt taagccacaa ctcgtgagcg cgcagcagca gctcggcgaa
     6061 gtccaccagg cctgcacgat cgcacgcttc ctgatacgcc tgatacacct tctgccaggt
     6121 ctgctccacc ggattaccgt agctttgaat atgatgcgga cgcaggcctt catctttctg
     6181 gctgttgatg taccacattg cctggcgcgg cggccactgc ttctcgtcga ggttcatggc
     6241 tttgatcaga cgcttaagca ggcgtagctg gtcttcgcta tcgaggatct ggaaatcctg
     6301 cggcagattg gcgtccatat ggtgcgcacg cagcaaacgg tgcgccagcc cgtggaaggt
     6361 gccgacccac atgccgccct ggctggtgcc catcagttgc ccgatacgat gacgcatctc
     6421 cgccgccgct ttgttggtaa acgtcacagc cataatcgag tatggcgagc agttttccac
     6481 gctcatcagc caggcgatac gatgcaccag tacgcgcgtc ttaccactgc ccgcgcccgc
     6541 cagcaccaga aggttgctgc gcggcgcggc caccgcttcg cgctgtttgt cattaaggct
     6601 gtcgagcagg taagaaacgt ccattggcac cgccgcgtaa aaataggttg ggcggagaag
     6661 cgcaacgccc tccgccaaaa agctgggtat atatacagat ttgctgatta tatcagcgag
     6721 gtcagagatg ccaaccggga aatttccaga tgcgggagta aacggctatc ccaggtttgc
     6781 atcagatcgc cattttccgg tctgatccaa caggcctgca ttccgctgcg aattgcccca
     6841 cccacgtcag tggtgagatc gtcacctaca tgtaagatct cgccgatcgg cacgttgagt
     6901 ttttccgccg ccaggaagta catatcgctg aacggtttcg agcgcccgtg cgggccagcg
     6961 cgcagcacaa actcaaaata atcccccaaa ccaaacagtt ccggctgagc gttaccgttg
     7021 gtgatcgcca ccagcggcca tttcttcgcc agctgtttta aggtgtcgtg ggtttgctgc
     7081 gggacgtcga tcctgctgcg ccatttagca aagttgatca ttgccgcgtg tgcgcctgca
     7141 ctggcttctt ccgcgctcaa tccggcgtca agcatcgctt gttctattga gcgaaaacgc
     7201 cagcgcgtca cgtcgtgata aatctcgggt tccgcttccc gtaccgcctg gcgcaggcgt
     7261 tgcagatctt cattctggaa gctgcgcagc gccggatggt aattttgcac aaaggtaagc
     7321 gcctcctgct cggtgcgcaa aatcaccgga cggttatcgt aaagggtatc atccaggtca
     7381 aaggtgagcg ccgagatgcg ccccaaaggc cggtaaaaac gcattatttc ccccgtttgg
     7441 cgcgtggatg cgccgcatcg tacaccgagg caaggtgttg aaaatcaaga tgagtataga
     7501 tttgcgtggt ggagaggttg gcatgaccca gcagctcctg cacaccacga agatcgccgc
     7561 tcgactccag catatgcgta gcgaacgagt gacgtaattt atgcgggtga acgtgattat
     7621 tcagcccttg ttttatgccc cattcggcaa agcgtttctg cacattacgc gcggagatac
     7681 gcttgcccag tttcgacaga aaaagcgcgt cgtcttcgct accaaacagg tcgcgcaaat
     7741 caagccagtg ctcaatccat gacaaagcgt tacgaccaat cggcaggcgg cgctctttgc
     7801 tgcctttccc catcacccac acttcgccgg actccaggtc gaggtgtttg atgtccagcc
     7861 ccaccagctc ggaaagacgc aggcccgcgc cgtacatcac ttccagcatt gcacggtcgc
     7921 gcacagcgag gggatcgttg atatcaatat ccaacaggcg atttatatca tcgacgtcga
     7981 tatttttcgg cagatgacgt ggcgttttcg gtgccgaaac acctttcgcc gggttagctt
     8041 tgagttcgtt ctggctgacc agccagtcaa aaaagctacg tagcgcagag aggcgtaatg
     8101 cgaggcttgc cgcgcccagc cctttacggc gactgcgtac agcaaaattg cgcaccatcg
     8161 ctgcatcgca ttgctgccag ttttgcaggc cgttttcgct ggcaaaattg atgatcgcct
     8221 caagctgtcg ctggtagtta agcagagtta tcggactaag ctgccgctcc acgctcagat
     8281 aacgtaggta gcgttctaca tcggtgtgta aatcggtcat acgcgttcaa tccaacgctc
     8341 cagaagctcc ggcaacatca gcgcaatttc atgaagtaac tgcgttccct gcccttgttg
     8401 atagtgactg gcatcgcggc tggtgaacag caccacgcct aaatcagcat cgcttcccag
     8461 catcgacatc gccaccgatc ccaccgcttt cgcttccggt agtaccacca gcagctccgg
     8521 tccgttaagc ggccccagat aatgctgctc ctgccccaga cgctgaatac gcagcggttc
     8581 gaaagactga cggcttaatg ccagatgagt gtggttcgac ggcgcaccta agcgccagcg
     8641 atccggaaac aggcgcagac tcgcacctgc caggccgaga tcacgcgccc agcggtgaaa
     8701 gcgcatcagc atatcatcaa gactgctggc ggcggtgaga ctgcgctgca ggtagagtaa
     8761 gcgataaaac aggccttcgt tggcgatagc ctgttccatc aacagcgcca tgttctcttc
     8821 cagaacatga atatgattac gtgcgcgggc catgtgccac tcgaccaacg aaacggtacc
     8881 gcgtaccgga tgcggcacac gtatcgcttc tactgcgcgc gcattgcgga taaaaaactc
     8941 aggattttta atcagataat cgacaaccac ccggtcatca agctccgtga gtgtttcctg
     9001 cagttcttcc cctggttgct tcatagatga ataaatccgt cgtagacatg taccgccggg
     9061 ccagtcatat ataacgggtg acccggacct ttccaggcga tatcaagacg accgcctggg
     9121 agttccacgc gtacttcttc ggccagcaaa ccttgctgaa tccctactgc aaccgccgca
     9181 catgcgccgc tgccgcaggc ctgggtttct cctgccccac gctcataaac gcgtaaacga
     9241 atatgctcgc gcttaaccac ttgcataaaa ccgatattgg cgcgctccgg aaaacgctcg
     9301 tggctttcca gaacaggccc cagtgtttct accgccgcag tatcgacatc atcgacctga
     9361 atcacgcaat gtggatttcc catcgacacc acgccgcata agattgtctg ctcggcggcg
     9421 cgcataatat aggtcttttc cgctttgtta gcgcgaaacg gcacggcaga aggttcgaag
     9481 ttgggttcgc ccatatttac gcggaccaga tcatcatcgg tgacggtaag aaccatccgc
     9541 ccgttggcgg tgctgacgcg gatatcgcgc ttattggtca gccctttcag acgcacaaaa
     9601 cgggcaaagc agcgtgcgcc gttgccgcac tgcgccactt cactgccatc agcattgaaa
     9661 atgcgatagt gaaaatccag ttcaggatca tacggcggct caaccaccag cagttggtca
     9721 aaccctaccc ccaggtgccg atcagctagg cgacgaatca gctccggtga aaaaaagaca
     9781 ttctgcgtta ccgcgtcgac gaccataaaa tcgttgccaa ggccatgcat tttcgagaac
     9841 tgcatcattt actccaatca cgcgggtaca gaaactgact tttagtaatt cacctgggat
     9901 ggaccatcgc cagtggcgcg atcgttttta tccggcaccg tggattgcgt ttgcgtctct
     9961 accggtttgg tcggcggcgg tgcattttta tctgcaggcg ggaaatagag cggacctttc
    10021 agaccgcagc ccgtcaggct gaagagagta agtaatacag tgagtgcctt aaacacgttt
    10081 ttcattatgg attgcctgta aagttcatcg ctttctgctt tctatcatcg caggagaagg
    10141 cgtaaaagca agcgtttagc gactctttat gaagtctgaa aagtgaggaa atgctgcgag
    10201 tataacgctt actcgtgagg atttatgttc tgctggcagt ttcgtgatca acacctcacg
    10261 ccaggatggc tttatgcact atcgcaatgt ggtttgtttg ttggcttttt tagttccgtc
    10321 agcgtgagga tgctcgctcg gtaaacctga tcataatgtt taccagaaac agggaaaggc
    10381 gtggtgtaac tgcgccctaa tgaagagaca aatctttcgc tacacagaga catcattatt
    10441 ccggtgacgg atgaatcatg ccgtaacgca gccgcagagg tgcaaacttt gaagccctga
    10501 ccccatgccg ctatactgcc gccatcactt aaaaacagga tacaacaatg aacgacagtg
    10561 aatttcatcg cctggctgat caactgtggc tgaccattga agagcgtctg gacgactggg
    10621 atggcgacag cgatatcgac tgcgaaatca acggcggcgt gctgaccatt acctttgaga
    10681 atggcagcaa aatcattatc aaccgccagg aaccgctgca ccaggtatgg ctggcaacca
    10741 aacaaggcgg ctaccatttt gatctgaaag gcgatgagtg gatttgcgat cgcagcggcg
    10801 aaaccttccg ggatttgctg gaacaggcgg cgacgcagca ggcgggtgaa acagtcagtt
    10861 tccgctaaga ttgcatgccg gataagcctc gctttccggc acgttcatca cgaaaaatac
    10921 tgctgtaata gcggcgtatc gtgatcctga ttggcaggcg gcatgttacc gatagatttt
    10981 gtgcggaacg gaatcacctg ttcacgacca tcaaccttca caatctgata gaactgcggc
    11041 aggttgaagt tgatgaagct tgagccgtag gtaaaacggt catgcgatga cgagtagaag
    11101 cgactgacgt cacgtaccag ctcctctttg ctgccttcgc agtggtgata cacctcaacc
    11161 cgattgcttt cgtcgagaat gtagatatta aagccattct catcttgcgt ttcttcgaaa
    11221 aagaactgga tgatcccttc gctggcaaag ccgtccacca ccgccggtaa tttgacgtga
    11281 ttggtttcaa cctgcactga caggccgtgc agtttgttat gcgaaatagc gccataaaac
    11341 tcgatggcgt tctccagttt ctgtaccgat acattcaggc gttcgaagaa caagccccag
    11401 gtttgaccag aaacgcgcag cgccttgaaa cgcccggttt cctggcgggt gctggaaaga
    11461 cgcaattcaa tacactcaga aaccagttgc tgcacgcgag tacgaattaa gccgcgcaga
    11521 tgctggctat aacagaagac ttccacgcta tctggcggtg cggcgtcctg atgcattttg
    11581 ccgagaatag ttttcagggc ttcgatcatc gattgctcgc cgttgaagtg cagcgtacgc
    11641 acttcattcc acgagttgcg atacagcagg tcaacgctac ctaccaggca attttgattc
    11701 tcgccaaagc tgaagacatc cagcttacgg aaatcaaaat gcaccacctg attgcggaac
    11761 gcagctgtcg ggtcatattc caggttaacg ataatcgcca gatggcggat ctcacacggg
    11821 ctgtagagcg ccttcggtgt cggtgcaggt aagcgcaacg ggaaatggtg cgacacgtcg
    11881 gcgaccatct cctgcaactt aggcaaatcg acaatgccgt tacctttaat atacaagcgg
    11941 gtgcgcgagg tcagcaggcc gttaaaccat gcccacgcca ccagtttatt caggtaacgg
    12001 ttatattcca gcggctgatg gctgatgatc gactcaatat ttggcgcgcg gttatacaga
    12061 taccaacctg aacggttagc ccggcccggc ggcacataaa taaaggtcag attcggttcc
    12121 gagagatcgg gtgaaatctg cgggtttacc agcgtcactt tacctggtaa tgcttcaaac
    12181 gcggcataca gcttacgcgt cagcacgccg atatcctgcg gactggcgga gacgctaagg
    12241 ttattgcgac gcgcaaagcg gatcagatta cggtagctct gcatcatcgc gtcgagcaac
    12301 tcgttgtgcg cctcacgcac ctgatcaatc ttccagttag cgcggttatc gagcattgcc
    12361 agacgagctt cgtcccaccc ccactcgctc actaactggc tcaacactgc gcgacgccag
    12421 cctacgcagg cgcgttcacg gctgagcttt tcgcacactt ttaaatagaa gcagcgacgt
    12481 actaaatcca gacgggtgaa atcttcaatc gccgtcaggt attcagtaac acgctccagc
    12541 atcatgcagt aaggatcgag gccaaacgat acaatctcgc cgtcgtgcaa acgctgtttg
    12601 atatctttcg ccagcaggcg tgggttcggg tattcccagg aataggcttc cagcagcagt
    12661 gtcttcagca ccgctttgta tggagaatcg atactcttgt agagttgcca aaggctggca
    12721 ccgaagtatt cttcagcgga aagcgagctt aagccgccga gatccagcca ttcatttggc
    12781 gtcagcacgc cttgtgcgta gagcgtcatt acgtagtcat cgtaatgctc ttcttcgtcg
    12841 cacggcacca tattccacag aatacgctta ccggcgagac gcacggcggt acgataaaat
    12901 tcatcaagca gcagtatatg ctgggtggag ccacaatctt cgccccccag actgccactc
    12961 tcgttgtggc gaaaacggtt ttcatcaatc aggaagaagc taacttccac acccagcgag
    13021 gcggcccagc tttccagcag gctgcattta cgttgtagca actggcgctc ttcgctatcg
    13081 agccaggatt gatgacagac ccagatatcc aggtcagagg aacaactttg ccctacggac
    13141 gaggtgctgc ccatggtgta tacaccagta attggaagct cacctttcgg cgggtcctgt
    13201 actgacattc cacgatacag ttcaagctcg ttcaggtagt ggcgttgagt ttcatcaggc
    13261 gtgtaaaggc aaatgccttt gggaacgtta ccatcaaggt aacccggcat tagcggatgg
    13321 tgatagtgca acaatgtcgg cagtagactg tagacctgtt ggaatgcagg ccccatagca
    13381 gcaagcgcgc gatccacacg caattgattt atggcatcca gtctctgttt cagagtctca
    13441 atatagaggt acaagacgta tcgcctgatt tgctacccgt catgactgtg attccgccaa
    13501 catcaacggt aacacgcggc attcgggata tttcgtatgt caaaggtaac cgttaccact
    13561 tttcgcgcct ggttttttta gtttcacgac gaaaaaatgg tctaaaacgt gatcaattta
    13621 acaccttgct gattgaccgt aaagaaagat gcgctacata caagtgtagc accgtttatt
    13681 ctctgtaaat tccttattac gacggcgtga aacgcctgtc aggatccact gccagacctc
    13741 attttacggt ttgcgcaggc gtctacgttt caccacaaca ctgacatcac tctggcaagg
    13801 atgttaggat ggaccacgga tgataatgac ggtaacaagc atgttagaca atgttttaag
    13861 aattgccaca cgccaaagcc cacttgcact ctggcaggca cactatgtca aagacaagtt
    13921 gatggcgagc catccgggcc tggtcgttga actggtaccg atggtgacgc gcggcgatgt
    13981 gattcttgat acgccgctgg cgaaagtcgg cggaaaaggc ttatttgtaa aagagctgga
    14041 agtcgcgctc ctcgaaaatc gcgccgatat cgccgtacat tcaatgaaag atgtgccggt
    14101 tgaattcccg caaggtctgg gcctggtcac tatttgtgag cgtgaagatc ctcgcgatgc
    14161 ctttgtgtcc aataactatg acagtctgga tgcgttaccg gcaggcagta tcgtcgggac
    14221 gtccagttta cgtcgccagt gccaactggc tgaacgccgc ccggatctga ttatccgctc
    14281 cctgcgcggc aacgtcggca ctcgcctgag caaactggat aacggcgaat acgatgccat
    14341 cattcttgcc gtagccggac taaaacgttt aggtctggag tcccgcattc gcgccgcgtt
    14401 gccacccgag atttctcttc cggcggtagg acaaggtgca gtcggtattg aatgccgcct
    14461 tgatgataca cgcactcgcg agctgcttgc cgcgctgaat caccacgaaa ctgcactgcg
    14521 cgttaccgca gaacgcgcca tgaatacccg tctcgaaggt ggatgtcagg tgccaattgg
    14581 tagctacgcc gagcttattg atggcgaaat ctggctgcgt gcgctggtcg gcgcgccgga
    14641 cggttcgcag attattcgcg gtgaacgccg cggtgcgccg caagatgccg aacaaatggg
    14701 gatttcgctg gcagaagagc tactgaataa cggcgcgcgc gagatcctcg ctgaagtcta
    14761 taacggagac gccccggcat gagtatcctt gtcacccgcc cgtctcccgc tggagaagag
    14821 ttagtgagcc gtctgcgcac actggggcag gtggcctggc attttccgct gattgagttt
    14881 tctccgggtc gacaattacc gcaacttgct gatcaactgg cagcgctggg ggagagcgat
    14941 ctgttgtttg ccctctcgca acacgcggtt gcttttgccc aatcacagct acatcagcaa
    15001 gatcgtaaat ggccccgact acctaattat ttcgccattg gacgcaccac cgcactggca
    15061 ctacataccg taagcggaca gaagattctc tacccgcagg atcgggaaat cagcgaagtc
    15121 ttgctacaat tacctgaatt acaaaatatt gcgggcaaac gtgcgctgat attacgtggc
    15181 aatggcggtc gtgagctaat tggggatacc ctgacggcgc gcggtgctga ggtcactttt
    15241 tgtgaatgtt atcaacgatg cgcaatccat tacgatggtg cagaagaagc gatgcgctgg
    15301 caatcccgcg aggtgacgac ggtcgttgtt accagcggtg aaatgttgca gcaactctgg
    15361 tcgctgatcc cacaatggta tcgtgagcac tggttactac actgtcgact attggtcgtc
    15421 agtgagcgtt tggcgaaact cgcccgggaa ctgggctggc aagacattaa ggtcgccgat
    15481 aacgctgaca acgatgcgct tttacgggca ttacaataac tctcataaca ggaagccata
    15541 atgacggaac aagaaaaaac ctccgccgtg gttgaagaga ccaaggaggc cgtggacacc
    15601 acgtcacaac ctgtcgcaac agaaaaaaag agtaagaaca ataccgcatt gattctcagc
    15661 gcggtggcta tcgctattgc tctggcggcg ggcgtcggtt tgtatggctg gggtaaacaa
    15721 caggccgtca atcagaccgc caccagcgat gccctggcta accaactgac ggcattgcaa
    15781 aaagcccagg agagccaaaa agtcgagctg gaaggcatta ttaagcaaca agctgcacag
    15841 cttaagcagg cgaatcgtca gcaagaaacg ctggcaaaac agttggatga agtccaacaa
    15901 aaggtcgcca ccatttccgg cagcgatgct aaaacctggc tgctggctca ggccgatttt
    15961 ctggtgaaac tcgccggacg gaagctgtgg agcgatcagg acgtcacgac cgctgcagcg
    16021 ttgctgaaaa gtgcagacgc cagcctggcg gatatgaatg acccgagtct gattaccgtt
    16081 cgtcgggcaa ttaccgatga tatcgccagc ctttctgcag tatcgcaggt ggattatgac
    16141 ggcatcatcc ttaagcttaa tcagctttca aatcaggtag ataacctgcg tctggccgat
    16201 aatgacagcg atggttcgcc gatggattcc gacggtgaag agctttccag ttccatcagc
    16261 gaatggcgta tcaatctgca aaaaagctgg cagaacttta tggacaactt cattacgatt
    16321 cgccgtcgtg atgacaccgc cgtaccgctg ttagcgccaa atcaggatat ctatctgcgc
    16381 gaaaatattc gctctcgcct gctggtcgca gcacaagctg taccgcgtca ccagcaagag
    16441 acttatcgcc aggcgctgga gaacgtctcc acctgggtac gtgcttacta cgatactgat
    16501 gatgccacca ccaaagcgtt cctcgacgag gtagaccagt taagccagca aaatatctcg
    16561 atggatcttc cggaaaccct gcaaagccag gcgatgctgg aaaaactgat gcagactcgc
    16621 gtgcgtaacc tgctggcaca accggcagcg gggacaacgg aagctaaacc tgcacctgca
    16681 cctgcacctg cacctgcacc tgcaccgcaa gctgatactc cggcagccgc gccgcaagga
    16741 gaataaccat gctaaaagtg ttattgctct ttgtgttgct gattgcgggg atcgtggttg
    16801 gcccgatgat tgccggtcat cagggttatg tgctgatcca gaccgacaac tacaatatcg
    16861 aaaccagcgt cacgggcctg gcgatcatat tgattctggc gatggtagtg ctgtttgcca
    16921 ttgagtggct actgcggcgg atcttccgca ctggcgcgca cacccgtggg tggtttgtcg
    16981 ggcgtaagcg tcgccgtgca cgtaagcaga ccgaacaggc gctgctgaaa ctggcggaag
    17041 gcgattatca gcaagttgaa aagctgatgg cgaaaaatgc cgatcacgct gaacaaccgg
    17101 tggtgaacta tctactggct gccgaagccg cgcaacaacg tggtgatgaa gcgcgcgcca
    17161 accaacatct ggaacgcgca gcggagctgg ccggcaatga caccattccg gtagaaatca
    17221 cccgtgtacg tctgcaactg gcccgtaatg aaaaccatgc tgcacgccac ggcgtggata
    17281 agctgctgga agttacgcca cgccatccgg aagtattacg tctggcggaa caggcgtata
    17341 tccgcacagg tgcatggagt tcgctgctgg atattatccc atcaatggcg aaagcccatg
    17401 ttggtgatga agaacatcgt gcaatgctgg aacaacaggc atggattggc ctgatggatc
    17461 aggcgcgtgc cgataacggt agcgaaggtt tgcgtaactg gtggaaaaac caaagccgga
    17521 aaacgcgtca tcaggtagcg ttgcaggtgg caatggcgga acatcttatt gaatgtgacg
    17581 atcatgatac tgcccagcaa attatcatcg atggcctgaa acgccagtac gacgatcgcc
    17641 tactgctgcc gattcctcga ctgaaaacaa acaatccgga acagctggaa aaagtgctgc
    17701 gccagcaaat caaaaacgtc ggcgatcgcc cgctgttgtg gagcacactg ggccagtcac
    17761 tgatgaagca cggagaatgg caggaagcat cgctcgcctt ccgcgcagcg ctgaaacaac
    17821 gtccggacgc ctacgattac gcatggcttg ccgacgcgct ggacagactg cacaagccgg
    17881 aagaagctgc agcgatgcgt cgcgacggtt tgatgttaac gttgcagaac aacccaccac
    17941 agtagttcct tctcacccgg aggcaagcac ctccgggacc ttcctgacac ataaaaaaac
    18001 gcctgctctt attacggagc aggcgttaaa acaggtctgt atgacaacaa gtgggtgctt
    18061 cactcaacgt tgtgtccatg gtgtctgatg aggcataagc gacatctgtc agtggacgat
    18121 aagcaccgta aacggctctg cgtcattccg gagtttatga ggcactaagg cgaacataag
    18181 agatggaatg agcatctact cgtttattat gccacagaga atcgggaaat aacatccctt
    18241 aacacttgtt atgagataat tctgtaatcc tctttgcttc ctgagtaata acttcctgag
    18301 tgaatattta acctgagctt gatcctacac atacttatta tgaatgataa aattcattca
    18361 attaataaca catatattaa ttgccgttaa aactaaaaac agcatcaata atcaacgcga
    18421 tataataaac ctgccttaca tatcaactgc gccagaggta ggattgaaaa cgctctcctg
    18481 atttttaaat tcatttcttg gataaataaa taatttattt ttatcactat tatttatgta
    18541 atcatcctgt cagggagagg gatctcaatt atcaatgctt aattacgtca tcattttgtt
    18601 gacatgaaac cggagcattt tgcatggaat tttcgttttc acccaaactt cttgttgttg
    18661 ctgtcgccgc cgctcttcct ctcatggcca gcgcagcaga taccccgtca actgccaccg
    18721 cgcgcaaagg ctttgccgga tacgatcacc caaaccagta tctggttaaa ccggcgacca
    18781 ctattgccga caacatgatg ccagtaatgc agcatccggc gcaggataaa gaaccccagc
    18841 agaagctggc agaacttgag aaaaaaaccg gtaagaaacc gaatgtggtt gttttcttgc
    18901 tggacgatgt gggctggatg gacgtcggtt ttaacggtgg cggcgtggcg gtgggtaacc
    18961 ctacaccaga tatcgacgcc gttgccagcc aggggctgat tttaacttcg gcgtattctc
    19021 aaccaagctc ttccccaacc cgcgccacca ttctcaccgg acaatactcc atccaccacg
    19081 gcattctgat gccgccaatg tacgggcaac cgggcgggct gcaagggtta accacgctgc
    19141 cgcagttgct gcacgatcag ggctacgtca ctcaggccat cgggaaatgg catatggggg
    19201 aaaacaaaga gtcgcagccg cagaacgttg gctttgatga tttccgtggc tttaactcgg
    19261 tgtctgatat gtacaccgaa tggcgcgacg ttcacgtcaa tccggaagtg gccctgagtc
    19321 cggaccgttc tgaatacatc aagcaattac catttagcaa agatgacgtt catgcggtgc
    19381 gcggcggcga acaacaggcc attgccgaca ttacgccgaa atatatggaa gatctggatc
    19441 aacgctggat ggaatatggc gttaagttcc tcgacaagat ggcgaagagt gataagcctt
    19501 tcttcctcta ttacggcact cgcggctgtc acttcgataa ctacccgaac gccaaatatg
    19561 cgggtagctc tccggcacgc acctcgtatg gcgactgcat ggtggaaatg aacgatgtgt
    19621 tcgctaatct gtataaagca ctggagaaaa acggtcagct tgataacacg ctgattgtct
    19681 ttacctccga taacggaccg gaagccgaag taccgccgca cggacgcaca ccgttccgtg
    19741 gtgcgaaagg atctacctgg gaaggcggcg ttcgcgtacc gactttcgtt tactggaaag
    19801 gcatgatcca accgcgtaaa tctgacggta ttgttgatct ggcagatctc ttcccaacag
    19861 cactggatct ggcggggcat cctggtgcga aagtggcgaa tttagtgccg aaaaccacct
    19921 ttatcgatgg tgtggaccag acatctttct tcctgggaac aaatggtcag tctaaccgta
    19981 aggccgagca ctacttcctc aacggtaaac tcgctgctgt gcgtatggat gaattcaagt
    20041 atcacgtcct gattcagcaa ccttacgctt atacccagag cggatatcag ggtggattca
    20101 ccggcacagt aatgcaaaca gccggatcgt cggtgtttaa cctctacacc gatccgcagg
    20161 agagcgactc tatcggcgtg cgccatattc cgatgggtgt accgctacag accgaaatgc
    20221 acgcgtatat ggagatcctg aaaaagtatc caccacgcgc gcagattaaa tctgattaag
    20281 ccggcgctat cgctgagatc tgcctttgcc ggatgcgatg ctgacgcatc ttatccagcc
    20341 tacagaacgc tgcaatttat tgaatttgca cgatcatgta ggccggataa ggcgtttacg
    20401 ccgcatccgg caatcaaccg caggcgacca caaaatggcc gcttttttac ttactcacca
    20461 ccagcaaatg cgcctgcata atgtcgctgg ccgggcgacc gtgcgccagc aaatcagcca
    20521 ttgctttaag atatggcggt agatggcgga aataacgctg atacccggca cacaaataat
    20581 tcagtcccgg tttgccgctg gcatcgagca tgaagcggtg tttcgggcag cctccccaac
    20641 atgcttttaa cacgttacaa ctgcgacact gcgccggtaa ttgcttaaat ttatcttcac
    20701 caaacacctg ctgttgcggg gaatcgatca tttctgcaat tgtttgctgg tgcatattcc
    20761 ccagtcgata ttgcggataa acatagtgat cgcaggcgta aacgtcgcca ttgtgctcaa
    20821 caatcaccga gcgcccacag gttggctgat gatggcaaac cgcacccggc gcaccgacaa
    20881 aattggcaaa cgcccattcg atattcatca cgaaaatctt gccgacgtcg cgtttaatcc
    20941 agtggtcgaa tatcgccacc agaaattcac caaattcatc ggggcgcaca gaccattccg
    21001 tcagttcccc ctgaatatca ccaggcgcat gtagtttcag tccttcgcgg gcagtcgttt
    21061 catcagccag gcgctcgacc accggaataa actggatgaa ttcgactccc gcatcgcaca
    21121 aaaaatcata aacctgtaac ggttgctgcg cgctggtgcg gttgacgcag accagcacgt
    21181 tatagtcgac atgatgtttt tgcaggagcg tcagggcacg catcaccagt ttatgcgtgg
    21241 gtctgccgcc ttttgtcacg cgatattgat tgtggatctc agccggacca tccagcgata
    21301 acccaacaag aaaatgattt tctgccagaa atgcacacca ttcatcatcg agcagtacgc
    21361 cgttagtctg gaagctgtta cttatcttcc tgccagcacc atatttcgcc tgtagcgcca
    21421 cggcacggcg gtaaaactcc agcccgagta gcgttggttc gccgccctgc caggtaaaag
    21481 ccacttcgtt ttgcggttcg ctggcagcga tatagtgacg gacatacgct tccagcgtgt
    21541 catcgtccat atgcgtgact ggcttttcgc ggtaaaggga ttgtttttcg agataaaaac
    21601 agtagtcaca gttcagatta caatcggaac cgctcggttt cgccatcaca tgaaaagcac
    21661 gcgttggaac ctgttgcagc atggtcgctc cttgcctgaa gtcatccttc aatacgttaa
    21721 gggcgaaatc ataccgtggg gcaatgagtt attacgggcg tttattgcga agggagggtg
    21781 acgaaatgca cagaaaacaa aaaaccccgc cgaagcgggg ttcaaaattg gtcggcgaga
    21841 gaggattcga acctccgacc cactggtccc aaaccagttg cgctaccaag ctgcgctact
    21901 cgccgaaata ctgctttttg aatttttagt tcaattcttt aaagtcgtgg tgcgaggggg
    21961 gggacttgaa cccccacgtc cgtaaggaca ctaacacctg aagctagcgc gtctaccaat
    22021 tccgccacct tcgcattgtc acaacttcta ataatggggt ggctaatggg attcgaaccc
    22081 acgacaactg gaatcacaat ccagggctct accaactgag ctatagccac cactacaaat
    22141 cttgttacgc ggtattacta ccaccgcagc tcaagcgccg ggactaaatg gcgcgcccga
    22201 caggattcga acctgagacc tctgcctccg gagggcagcg ctctatccag ctgagctacg
    22261 ggcgcttagc gccgttgcgg gggtggataa tacggacttc ccccccctct gtctagtccc
    22321 tttttaaata aattgcgcgt ttggttacgg tttgtgcgtt ttgctgctta ttcctccagt
    22381 ttatgcgctt tcccgtggcg attaaggcca aaaactttat aaatcgccgt caccgccagc
    22441 ataaagatga tgccaacaaa cagcgacata cgcgtatctt cattaaagta catgccgatc
    22501 aaaacgcaaa tcaggaatgc cattgttacg tagttggccc acgggaacag gattgagcgg
    22561 aacggatggc tggcaatcgc cgctttatgt gcacgccgaa aacgcagctg gcttatcaat
    22621 atcacaaacc atggcaccat ccccggaagc acgctggcac tgtagacgta gacaaacaca
    22681 cgctgcggat tgggaatgat gtagttcagg catgagccaa ttagcagaat agcaatagat
    22741 actgccacac ccgcaaccgg tacgccgtga cgggaaactt tcgccatcgc cgccggtaac
    22801 tgacggtttt tcgccagtgc gtagagcata cgtccgcaac tgtacatgcc gctgttacag
    22861 ccagagagcg cagccgtcag caccacaaag ttgataatgc ccgccgctgc ggtaataccg
    22921 attttggcaa aagtcagtac gaacgggctg ccgttgctgc ctatttcatt ccacgggaag
    22981 atggtgacga taacgaaaat cgcgccaacg tagaaaatca ggatccgcca cagcaccttg
    23041 cctacggcac tgcgcagcgt cacctgcgga ttcttcgctt caccggcagt aatgccaatc
    23101 agctccacgc cctggtagga cgccaccaca atacacagcg cggtcaggaa ccctttccag
    23161 ccacccgcaa agaaaccgcc atgctctgtg agattgctaa aaccaatcga ctgcccgcca
    23221 ttgccaaagc caaagaaaat cacgcccagg ccaatgacaa tcatcacgat aatcgtggtg
    23281 actttgatca tcgcgaacca gaactcgatt tcgccgtaca accgcaccgc tgccagattc
    23341 gccaacgcca ccagcgccac tgcgatcaat gcgggtatcc actgcgccat ctccgggaac
    23401 cagaactgga cataaacgcc aatggcggtg atttcagaga tccccaccgc catccacata
    23461 aaccagtaag accaggcggt gagatagcca aagaacgggc tcatataacg atgcgcataa
    23521 acggcgaacg aaccggtaac cggttcgagg aacaacattt cgcccattga acgcatgatg
    23581 aaaaagacga acagcccggc gatgatatag gccaacaata cggatggccc ggcccatttc
    23641 agggtactgg cggcccccat aaacaggccg acgccaatgg tgccccccag ggcgatgagt
    23701 tcgatatgtc gagcttccag cccacgctgt agctctggtt tgttatctgc cataaatcct
    23761 cgtgttgtgt ttgcatgctt tccggtgtta ccggttatcg ttatgggtac atcgagtgtt
    23821 gcgaatgttt tcgtaattca ggagaaatgg caaataaagc attaaaaatt tgaatgcttt
    23881 gtgtaataaa aaagcagaca ggcgacggag tgaccactcc gtcgctttac aaagagagga
    23941 aaatcagagg ttgccggtgt aatgccagcg taaataacgc agcaaacgaa gctgacgctt
    24001 aatgcggctc ggctgcgaaa gcaggcggta gagccactcc agccccagcg tttgccagat
    24061 tttcggtgcg cgttttacgt gaccggtgaa aacatcgtaa gtcccgccaa cgcccatata
    24121 cagcgcatct ggatgcacca gacggcagtc gcgcatgaag atctcctgct ttggcgatcc
    24181 catcgcaacg gtgacgattt gcgctccgct ggcatgaatg cgttcaaaca gcgcctgacg
    24241 ctgctcgggt ttaaaataac catcctgact gccaacgata ttcacattcc actggttgcg
    24301 cagtttagct tcagtttgcg ccagcacttc aggtttaccg cccacaagaa ataccgtcgt
    24361 cccttctttg cctgcgcgcg ccatcagctc ttcccagaga tcggcaccgg caacgcggga
    24421 aacctgcgcc tgcgggtact ttttacgtac tgaacgtaca acgctgatgc catccgcata
    24481 tttaaattcg gcagcgttaa ttaactccct gacctcggcg ttctcttcaa tggtcagcat
    24541 tttttcagca ttaatggcaa ccagcgttcc ctgcttaagc tgcccgtcag caaacagata
    24601 atcgagggca tgctgcatat cacgccaacc aatcaactgt aagccacgca gcgtataggt
    24661 cggtgccgtg gtgttgttat tcattgttat ccttcaacct gcgtccggag cgatgatttt
    24721 gtacgtttat gaatgagtcc ggcgctttca aaaagccagt acaacagttt tgcgatcatc
    24781 agacatgcgc cgaagaccac gataaaaaag accacgcgtg agacaaacga atccagccct
    24841 tcacgcgcca gcacgatcat attgaagatc gccccaaagc agaaactgtg caatatcgca
    24901 gccttatagc gattagtttc gcggttgccc agctcataca gccagtcgaa ccatttgatg
    24961 atcagtccaa ccacaatcgc cccgagcggg atgaacaacg cgccgcccat caccaccagt
    25021 gagcctataa gcgtaggcga gatcgccagt ccggagtggt tattcagcac ttcccaggta
    25081 aagtagttgg ctgagttcag caccatactc gggcgacccg gccacagcca ggaagggata
    25141 aagacataga aatcgcggac aattggcgcc aggccctgga agtcgatgtt gtcgtagttc
    25201 tgcaacagca acgccagatt ctcccacggc gagaaggtgt cgcgagtgag atagagaaac
    25261 gtatagaacg cttcatcgcc gctcacattc attccatagc gttttagcgc cagccagaac
    25321 atgccaacaa tccccagcac gcccgccgcc gccagcatcc acaacgaaat ccagccgcga
    25381 ataatgccaa taaacaggaa gatagcgaat gcgatgatga tattggcgcg agtgccgccg
    25441 acaatcatat aagtcagcaa gccaaaggcg accgtgctga cgaggaaaaa cagccacgct
    25501 ttgctgtcct ggcgcagaaa gtagaccacc agcatcgccg ggatgaaaaa gtaaaagaag
    25561 cgttttaacg ccacgccgga gacttcactg gaaaagatct gactgtagga gttaagccgg
    25621 aacagcaaaa agccgttgtg catgaagaag atgccgacgc ttaccagcgc gatacccatc
    25681 aggatcaccc acgtaagatt ggtttccacg cggttcatgg taaacagcgg acggcgcggt
    25741 acatcagcaa cgcgtttgcg taggcgggtt ttgtaggtga cgtagtaaac cgcgtagaag
    25801 cagcccgcag aaagcaacgc ctgcaacagg atttctggcg gcgcgacacc aacatcaaag
    25861 cgaaacacca gcacgctggt cagcgggaag ccaaagaaaa aggtgagcag aaacagcaat
    25921 gaaaagaata cattgaagtt aaagcgcaca cgacgaaact caaaccaggt cagcgtggca
    25981 ataaacagcg tgcagagcag ccagacaaca aacaggccac tgaattgcag cagactcatg
    26041 cgacctcccc ggcggcaatc gccaacgccc gctgccagcc ttgtagatag ttagggctaa
    26101 agaaggcaat ggtgttttta tccaccgacg ccaactggcg ctgcgcttca cgcacaatat
    26161 cctcgttgag atcgtcggta gtaaacagca ccggtaaatg ctgttccgtc atatcctgcc
    26221 agaacggatt ttcccggtta agcacacaag gaatgcccgc ctgaatcagt aagcacagcg
    26281 taccaatgcc ctgctggcgg gcaaaaataa agtaaccaag atcgcactga cgaagtagcg
    26341 tcagataggc gtcaaattcc agtttttcac tcagaacttg cagattttct tcgctgaata
    26401 actccagccc cgcctgacga acttcctcga tgtacgcttc gttatgaggc ggatatccca
    26461 tcggcaccac cacttttacc gtatcgccaa attgctgatg aacggcgcgc aaggcagcaa
    26521 tatgctcatt gctgcggtcg ccggagttcc ccaccagaat ggtcattttc ccttcacgtt
    26581 gccgatcgtt cgccatcgta ttgagagaag catccatccg cgtcgggaag tacagcagtt
    26641 cgccccgcac ctttgggtgc gttttggcaa aaaagctcaa atcaccacgg gtggcaaata
    26701 cacagccgac tcgcttttgc gccaggcgac gtagtgggta aaaaagctta tatctcaagc
    26761 cactggaaag ctcgtacagg tctgcccccc agatatgcca gtaaaactgg ctgggcttaa
    26821 tgccaccact cagcagagcc agccacagtg tgggattgaa ctgaccgtgg aagaaaaaac
    26881 gctgctgacg gttagctttt gctttcgcga tgaccgcttc cgccagcgat tttttcccag
    26941 ggaaaaattg cacagaaagc gccggacagc tatcacttaa gccgtcgtcc ttgccaacaa
    27001 ccataaactc gcgcgcgtgc tcgctcgtcg cggccagcgc gtcattgaaa aaccgcaaaa
    27061 cggttcggtt atggtgaggg atatccgatc ccagtacgtg aatcagtaca gtcatgcccg
    27121 cctacgccag agtaaaaaca cgccacaaca aagagaaaaa tagacgatat aggttgccat
    27181 atatgcctgt gccgcgccca gcgcgccgtg cgtagggatt agccagtggg caaataccat
    27241 caataaagtg aactggctga cttccgccag aatataaaac cgcagtgaag ctttggcgat
    27301 caccagataa ccaaagacat aagcgcccac ttttaacaca tcacccacca actgccaggc
    27361 aaagagatcg cgcatagcgg taaatttatt cgacaacagc agccagatag caaaatcacg
    27421 cagcaaccag acggtaaaac tcgccgccgc cactgccggt aagacgaatt tcagcgattt
    27481 aaccacttcc cgggtgatat cgcgcttttc cgttagccgc gacaacgtgg gcagcaaata
    27541 tacgctgaac gatgccgtaa taaattgcag gtaggcatcg gaaatactgc tcaccccttg
    27601 ccagatcccc acctcatccc agctatactg cgccgccagc agtttacgca tcatgatgta
    27661 agcaacaggc aaggtcaccg acgtaatcaa cgtcatgagc gtaaatttgc tcaactgccc
    27721 tgccagaccg ttatcccagc tgggtttcag atagcttaac gggatagcac cacgcttaat
    27781 taacatgacg gcggcaggaa tcaccaccag cgcgggaatc aacgccagac ccagcaacgc
    27841 cccttcatat ccgcccaaac ggtaactgac gtagtacgca agaacgccaa tcaagctgcc
    27901 aacaatcaga gataacgcat tacctgcggc atcgcgaaag cctttcatca acgccagtaa
    27961 caggttgccc caggcgatcc ccatttgcac cagcgccact aaacgcacca gcccctgata
    28021 gtcgtgatta ccaaacaatc ccaagctgat tggcgcagct gccagcacaa aaaccagcgc
    28081 catcagcgta gagaagccaa gtaccatcgc tgatgaagtg ccgaccacgc ggcgcagctg
    28141 ttgcggatta tcatggtact gggcaacgta tttggttaca ccgttaaaga tgccagcccc
    28201 ggcaagcacg ccgagcacgg taatcaactg gcggaaattt gccgccagcc caagccccgc
    28261 cggaccaaat gacaccgcca gcaacttacc gaccagtaac ccggcaccaa tcttgaccag
    28321 tgtactggcc gccgtccaca aggacgcttt tgccaacgac atatcaggag aagtagttca
    28381 acaaagtcgc aattaccgta cgctgattga cgggcgacag gttgtagaac agcggcaggc
    28441 gcagcaggcg ctcgctctct ttggtggtgt agcgatcttc accgtggaac tcaccaaagc
    28501 gttcccccgc agggcaaccg tgcagcggaa tgtaatggaa caccgccatg atttccgctt
    28561 ctttcagaaa gttaatcaac gcgctccggt caccaatatc ccgcagttta atgtaaaaca
    28621 tatgcgcgtt ctgcaagcag ccatcgggaa tcgacggcag ctcgatacgc ccggctttcg
    28681 ccagaggtgc taacgcatcg tagtagtttt gccacagcgc cagacgttgc tggttgatac
    28741 gatccgctgc ttccagttgc gcccacaggt acgcagcttg cagatcggac atcaaatagc
    28801 tggagccgat atcgcgccag gtatatttat cgacctgacc acggaagaac tggctgcggt
    28861 ttgtgccttt ttcacggatg atctcggctc gttcgatcaa cgctttatcg ttaatcagcg
    28921 tcgcgccgcc ttcaccgccc gccgtgtagt ttttggtttc atggaagcta aagcagccaa
    28981 tatgaccaat ggttcccagt gcacgccctt tgtaagtgga catcacgccc tgagcagcat
    29041 cttccaccac aaacagatta tgctttttcg ccaacgccat aatggtgtcc atttcgcagg
    29101 ccacgcccgc gtaatgcacc ggcacgataa cgcgcgtttt gtcggtgatc gccgcttcaa
    29161 tcaacgtttc gtcgatgttc atggtgtccg ggcgaacatc cacaaaaacg atttttgcgc
    29221 cacgcagcac aaaggcattg gcggtggaga caaaggtgta gctcggcatg atcacttcat
    29281 cgccaggctg gatatcgagc agcagcgccg ccatctccag cgaagcggtg caggacggcg
    29341 tcagtaacac tttggcgctg ccaaaacgtt gctccagcca ctgctggcag cgacgggtaa
    29401 aaccgccatc gccacacagt ttgccgctac ccattgccga ctgcatatag tcgagttcgg
    29461 ttcccaccac cggcggtgcg ttaaatggaa tcatgtgatc acctgtataa ccagtacgcg
    29521 gtgctttcta cattcgcacc actttgtatg tatcgtttaa gcgcggcggt gttgcccatt
    29581 tgggtcgcca cccgcaaagt tgttttaccg cgagcatacg cccagtttag cgccgtttgc
    29641 atcagctcag cacctgcacc gcgtccagcc agcaggccaa ttcgcgcatc tgtagcattg
    29701 agttcccgta aagagacata accgcgaata tcgccggacg ccgcacgtaa aatcagacat
    29761 tgatgatcaa aggtgccgcg cacggcattt tcaatccact gtgcataaaa gcgactgctg
    29821 gcgtcaggcg cataccacgg cgcacgaaaa cggctttgcg caaatgcggc gctggctaac
    29881 tgacgtaatg cgggaatatc ggtctcttgt gccactacag caccgctatc actggcattg
    29941 ttcacgggta gcgccaaatc aacttcacct tctaccaggg agaatcccag ctgttgcagg
    30001 gcatccagtt cccccgtatt tgatgccgca attttggcct gcacccgtga ccacggcgct
    30061 aacgcgtctg gcgtcaggaa cggtgcttca gacgtaatgc gcacgatggc gctgttaaca
    30121 ccaaagaagg cgttttccca ggttagtggc tcaatactgg cgcggacggg cacgaagtaa
    30181 ctccagcaga tattggccgt agccagtttt cgctaatgaa ctggcagcac gcttcacacc
    30241 ctcgtcatcg agccagccgt tacgccaggc gatctcttcc aggcaggcaa tcttaaagcc
    30301 ctggcgtttt tccaccgtct gtacaaaggt gctggcttca atcaggctgt cgtgagtgcc
    30361 agtgtccagc caggcaaatc cgcgcccgag cagttcaacg gtcaggttgc ccgcctcgag
    30421 gtacatctgg ttgatggagg taatctccag ttcaccacgc tccgacggct tcacctgctt
    30481 tgcgtactcc acgactttac tgtcgtagaa ataaagcccg gtcaccgccc agtttgattt
    30541 cggctgtttt ggcttttctt ccagcgagat agcgcggaaa ttatcgtcaa actccaccac
    30601 gccaaagcgt tccgggtcca tcacctgata gccaaaaacc gtcgccactt ccgtgcgcgc
    30661 cgcaacatga cgcagcttcg gactgaagcc ctgaccgaag aagatgttat cgcccagcac
    30721 cagacaagaa ggttcaccat taaggaaggt ttcaccgatg ataaaggcct gcgccagacc
    30781 gtccgggctg ggctgttcgg catattccag ctggataccg aactcactac catcgcccag
    30841 caggcgctgg aaataacctt tatcttccgg cgtagtgatg atgagaattt cgcggatacc
    30901 ggccagcatc agcaccgaca gcggatagta aatcattggc ttatcgtaaa tcggcaacag
    30961 ttgcttcgat acgccgcgcg taatcggatg caatcgggta ccggaaccgc ccgccaggat
    31021 aatacctttc atgtttgcct ccgctgaaaa ttagcctttc agacctaaac gctcgccctg
    31081 atagctgccg tcctgcacct gcttcctcca gctttcatta gccagatacc actgcaccgt
    31141 tttacgcatt ccactttcaa aggtttcctg cggcagccag ccaagttcac gggcaatttt
    31201 cgaagcatca atggcatagc gcagatcatg ccccggacgg tcagcgacaa aggtgatcaa
    31261 gtcacgataa tgcaccacgc cttgcggctt gttcggagcc agttcttcca gcagctcgca
    31321 aatggtttcc acaacatcga gattcttacg ctcgttgtga ccaccaatat tataggtttc
    31381 accgactttc ccggtggtcg ccacgcaata cagcgcgcgg gcgtgatctt caacatacag
    31441 ccagtcacgg atttgctgcc cgttgccata taccggtagc ggtttacccg ccagcgcgtt
    31501 gaggatcatc agcgggatca gtttttccgg aaagtggtaa ggaccgtagt tattcgagca
    31561 gttggtgata agcgttggca gaccataggt tcgcagccag gcgcgcacca gatggtcgct
    31621 gctggctttt gacgcggaat aagggctgct cggcgcatac ggcgtggttt cggtgaagaa
    31681 atcatccgtc gagtgcaggt caccatatac ttcgtcagtg gagatatgat gaaaacggaa
    31741 cgctgatttt ttatcttccg tcagcgcatt ccagtaagcc cgcgccgctt caagcaatgt
    31801 ataagtcccg acaatgttgg tttcaataaa cgctgccggg ccgtcaatag aacggtcaac
    31861 atggctttcg gctgccagat gcatgacaca gtctggctga tgctcagtga atacgcgcgc
    31921 cagttctgcc cgatcgcaga tatcaacttt ctcaaaggca aagcgctcgc tttgcgcgac
    31981 cggtgccagc gacatcaggt ttccggcgta ggtcagctta tcgaccacta ccaccgcgtc
    32041 gctcgtttcg ttgatgatat aacgcaccag cgccgagcca ataaacccgg caccgcctgt
    32101 tatcagaatc tttctcatca gcgccagact cctttagcat cgacgacata ctgctgatgg
    32161 acattgtcgc cattgataac tttgaactga ctatgatcga ccagcatcac cagcacgtct
    32221 gccgttgcca gcgcctcgtc aagctgcgcc agagtacaaa gcccggtcag ttttttcggc
    32281 aactggtgga tgttaggctc aacaaccaga gtttcgccgc tatgccactg ggcgatcagt
    32341 tcagcgattt ccatcgccgg gctttcgcgc aggtcatcaa tattcggttt aaacgccaga
    32401 ccaaagcagg cgattttcag ttcactggcg cgtttatcgg tagccgccag gcaatcagcc
    32461 accgccgctt tcacctgatc gataacccag aacggtttgt gatcgttcac ttcgcgcgcg
    32521 gtacggataa gccgcgcctg ctgggggttc tgtgccacga taaaccacgg atcaacagca
    32581 atgcagtgac cgcccacgcc agggccaggc tgaagaatat taacgcgagg gtgacgattc
    32641 gccaggcgaa tcagttccca gacgttaatc ccctgatcgg cacaaatcag cgacaattca
    32701 ttagcaaaag cgatattcac atcgcggaag ctgttttcgg tgagcttaca catttccgcc
    32761 gtccgcgagt tagtgacgac acactcacct tcgaggaaaa ttttgtacag ttcgctggcg
    32821 cgggccgaac aaaccggcgt cataccacca atcacgcgat cgtttttaat cagctcgacc
    32881 ataacctggc ccggtaacac gcgttccggg cagtaagcaa tgttgacgtc cgcctgctcg
    32941 cccacctgct gtgggaaagt gagatccgga cgcatctctg ctaaccattc tgccatcttc
    33001 tcggttgacc ccaccggcga ggtggattca aggatcacca gcgcgccttt tttcagcact
    33061 ggcgcaatgg agcgagcagc cgattcaacg taggtcatat ctggctcatg atcgccttta
    33121 aacggcgtgg gtacagcaat cagccaggca tccgcttcaa ctggcgtcgt gctcgctcgt
    33181 aaaaaaccgc cttctacggc agtttttact acgctcgcca aatcaggctc gacgatatgg
    33241 atttcgccac gattgatggt atcaaccgcg tgttggttga tatcgacacc aatcacctgt
    33301 ttttgccgtg aggcaaacgc tgctgctgta ggcagcccga tgtaacccag tccgataaca
    33361 gaaatggtcg caaaactcat agtgatatcc gattattttt taacgcttcc agaatgcgag
    33421 agcatgcctg accatcacca tacgggttat gggcgcggct catagtttga tattcgtttt
    33481 cgtcttttaa aagacgcgtc acttcctcga caattcgctg cttatccgtg cctaccagac
    33541 gcaccgtacc cgccgtcacc gcttccggac gctcagtggt atcgcgcatc accagcaccg
    33601 gtttccccag cgaaggcgct tcttcctgaa tgccgcctga gtcggtcaaa atcagccagg
    33661 cgtggttcat cagccagaca aacggtaaat actcctgagg atcgatcaga atgacatttt
    33721 tcacatgccc cagaatgcga ttgaccggct ctctgacgtt cgggttgaga tgcaccggat
    33781 agacaatctg gatgtcctgg tgcgtggtgg cgatgtctgc cagcgcgtgg cagatttctt
    33841 caaagccacg accgaagctt tcacgcctgt gaccggtcac cagaatcatc tttttatcgg
    33901 ggtcgataaa cgggtaattt gccgcaagtt ctgaacgcag cgtgtcgctg ctcatcacct
    33961 ggtcacgcac ccataacagt gcatcaatga ctgtattacc ggtaatgaag attcggctat
    34021 ctgcaacgtt ttcacgcagc aagttttgcc gggaagtttc ggttggagag aaatgataca
    34081 tcgccagatg cccggtcaat gtacggttag cctcttccgg ccacggcgaa tagagatcgc
    34141 ccgtgcgtaa accagcctca acgtgaccaa caggaatacg ctgataaaac gccgccaggc
    34201 tggttgccag cgtcgtcgtt gtgtcgccgt gaaccagcac gacgtctggt ttgaactcgg
    34261 caagaatagg ttttagccct tccagaatcc gacaggttat ctctgtcagg ccctgtcctg
    34321 gctgcattat gttgagatcg tagtcaggta caatggaaaa gagtttcagc acctgatcga
    34381 gcatctcccg atgctgcgca gtgacgcaaa ctttagcctc aaaaaaagga tcttttgcca
    34441 acgcatgcac caacggagcc atcttgatgg cttccgggcg cgtaccaaat acagtcagta
    34501 ctttcacatc gattctcttc gaataagcgg cgagcgcctt tgcgctcacc gcagcagtgt
    34561 tgctatttcg agcaacggcg ggttaatgcg acaccagccc cgatcagccc cccgacaatg
    34621 ccccacataa tcatcaggaa ggcacgacgt gggctatcgc gttttaccgg ttcttccggc
    34681 gtacgcaaat agcgataggt ctgaaaacgc ggatccaggg ttggaccaac attcagggtg
    34741 tttaacatgg cccgattctg atcatagtcg agatcaaagg ccggaccgac ggcctgtaaa
    34801 ttttccagtc gagcctggag cattggacgc ccaagcagga acatttctga atcaggtaat
    34861 tcctcggcag gcacatctgt cgcactgcgc gaaatattat gctgctcagc aattttcagc
    34921 gcctgctcaa tgctgttcat ccggcggtcg tagatggctt tcgccacctc ttcctgacgc
    34981 ttcacctgtg ctttcatctg gatggtacgc gccgcccatg cgcctttcag ctcatcattc
    35041 agatggctgg ctgcacgctg gctggcaaaa gcaacatact gacgtaacag gttattagcg
    35101 tcaggcgcgg tttcggcaat aagcttcacg ctgtcattga ccgcgcgggt aaagtctccg
    35161 gggataaact ggatgttgtt aatcatttca tccagcaacg ccgcatcggc tttgctgttg
    35221 cccaccatcc gctgcttgta atagtcggtt tgcagccaga actctctgcg ggtatcccac
    35281 gaggccagtt gcataacaaa ctctttatag gcttcgtcca tgaccgatgg ttggtcggca
    35341 gaagccatgt ttgaacggac atccaggtta cgcaaaaatt gctgctgcga gtaatatccc
    35401 cccagcatat tcaccgttgg acgatcggta atcgccgtcg agctccactc ctgacgagca
    35461 aaaaacgtat acgccagcgc gattaacgca aacgccagcc ccatgccaat aatccatagc
    35521 ttcccagccc acaaggtacg aaacaaccca cgaatatcca gttcattttc agcgtcttcg
    35581 gccggtttcc caggcattgg ttgtgtcatc acatcctcat ttatttggtt aaattggggc
    35641 tgccaccacg atttctacgc agtctgcgtt ttacgcgctt aataaagcga gcaactttcc
    35701 aggcacgctt aatgcaatat ccatagagga ggaatgctag caaaaagagc accaacatga
    35761 cccactccgg gacaaaatga gaatattctg ccagcacgcc aatggaagcg agcagtgctg
    35821 cggcaagggt aatcagcaca aacgcctggc gggaagtaaa cccggcacgc atgatcaaat
    35881 ggtgaatatg ctgacggtca ggagagaatg ggctcatgcc tttacgcagg cgacggtaca
    35941 taatcgccac catatccatt agcggaatgg cgattatcca caaagcggta accgggctga
    36001 tgggatgggt tttgccttgg gtcgtttcga gcaggatcca gatcacggta aaaccaatca
    36061 gcgtactgcc cgcatcaccc ataaagactt tgtagcggcg acccaggata ccaaggttaa
    36121 gcatgatata tggcaggatg gcggcgatca tcgcaaagca ccagattgcg aggctggttt
    36181 gcccgtcgaa ccacaaaatc ataccgattg ctgcaaacga gacgcaggac aacccgccca
    36241 gcaagccatc aatgccatca accatgttga acgcattaat ggccgcccag acggcaaata
    36301 gcgtcaggaa gtaaccaaac ggtccgagca ccatctccca ggagccaaag atataaccca
    36361 ggctactgag ataaaggttg ccaaacacca tcataacaat gccaacagcg gcctgtatgg
    36421 tggcacggat ttttacgctg atatcaaaac ggtcatccag cgcgccaatg aaaacaagca
    36481 caccggcaca agcgagatag agagatgcat gcggaatata gtaatcgaca attccgaacg
    36541 tgaagcaaat ccctgcgtaa accgaaatac ccccaacgag tggtatcaac ccctggtgac
    36601 gtttgcggaa gtttggttta tccactaaac cgactttttt tgccacctta cgggcaaaaa
    36661 acagaaacag tgtcgtgaat aaaaaaatac tgatgagatc agtactcact gtcagtaaat
    36721 tcacaatgca tgctctcaga gaaaattatt agcagaagta taaccacgaa gacctttatt
    36781 cagaagggaa agtccgttcc gaacccacca gtcctgtgca aaaattaacc atttgatatg
    36841 caaattgcat aacatgccag caaattccag gaattttcct aacatcgcca gcgcggcatt
    36901 aagattacag atataaaagc aaaacgccac gtaaacacgt ggcgtttttg gcataagaca
    36961 aatttatgag cgtttcatca tttcgaagaa atcgtcattg gtcttggtca ttgccagttt
    37021 attaatgagg aattccattg catcgatttc gcccatcggg tgaatgattt tgcgcaggat
    37081 ccacattttc tgcagttctt cctgagtcgt gagcagctct tctttacggg taccggaacg
    37141 gttgtagtcg atagccggga agacgcgttt ttcagcgatc ttacgagaga ggtgcagttc
    37201 catgttgcct gtacctttaa actcttcgta aataacttcg tccattttag aaccggtatc
    37261 gataagcgcc gtcgcgataa tggtcaggct gccgccctct tccacgttac gcgccgcacc
    37321 gaagaagcgt ttcggacgat gcagggcgtt ggcatccaca ccaccggtca acactttacc
    37381 tgacgccgga acaacggtgt tgtaagcgcg cgccagacga gtgatggagt cgagcagaat
    37441 gataacgtct ttcttgtgct caaccaggcg tttcgccttc tcgatcacca tttccgcaac
    37501 ctgaacgtgg cgagatgcgg gttcgtcaaa ggtagaagca acaacttcac cttttaccag
    37561 tcgctgcatc tcggttactt cttccggacg ttcgtcgatc agcagaacca tcagcacaca
    37621 atccgggtgg ttgtaagcaa tgctctgagc aatgttctgc agcagcatgg ttttaccggc
    37681 tttcggcggt gccacaatca gaccacgctg accacgaccg ataggtgatg ccagatccag
    37741 tacgcgagcg gttaaatctt cagtagaacc gttaccacgt tccatacgca gacgagagtt
    37801 tgcgtgcagc ggggttaagt tctcaaagag gattttgttg cgggcgtttt caggtttgtc
    37861 gaagttaact tcgttaactt tcagcagcgc aaaatagcgt tcaccttctt tcggcgggcg
    37921 aatcttacca gagatggtat caccagtgcg gaggttgaaa cggcggattt ggctagggga
    37981 aacgtagatg tcatcaggac cggcgaggta ggagctgtct gcggaacgga ggaaaccaaa
    38041 tccatcctgc aatatctcca gtacgccatc accaaagata tcttcgccac tctttgcgtg
    38101 ctgcttcagg atggcaaaaa taatgtcctg cttacgcata cgagccaggt tttccagccc
    38161 catattttcg ccgagagtga tcagctcaga aaccggcgta ttctttaatt cggtaagatt
    38221 catagtggtg tgagttctta aacttggggt aaatctcgaa cttaatgttg tgaatggtat
    38281 ggcaggatca tccatgcctg tttacggtca tcgactcatg tcttttcgct gcctggtcac
    38341 aggagagtac gcagaactga aacgacaaga cggattgagt gacgagccag aaatctgttc
    38401 acttcgcatt taagataaaa cgggaagcgt tggaaattac aagattcaaa cttaataagg
    38461 tatgtttaat acgaagtcaa cactaagtta gcatgactca cgccgggcgt ccagttttta
    38521 gcgacggggc acccgaacat gaaattccct tacgccaggt tagcgtcgag gaactctttc
    38581 aactgacctt tagacagtgc gcccactttg gttgccgcca cttcaccgtt tttgaacagc
    38641 agcagagtcg ggataccacg gatgccatat ttcggcgcag tgccagggtt ttgatcgatg
    38701 ttcagttttg caacggtcag tttgccctga tattcgtcag cgatttcatc cagaatcggg
    38761 gcgatcattt tgcacggacc gcaccactct gcccagaaat cgacgaggat cgccccgtcc
    38821 gctttgagta catccgtgtc aaaactgtcg tcagtcaggt gaataatttt atcgctcata
    38881 tataactcca caggaataag cctggcgtgt tggtttcgtt gttggtgtaa cattaaccaa
    38941 ctaaaggttg actttatttc accggatacg ctttcgtaaa gcaatagtaa gctgatattc
    39001 taccacacta tgagcaaaac acatttaaca gaacagaagt tttccgactt cgccctgcat
    39061 ccgaaggttg tagaagccct tgaaaaaaaa gggtttcata actgtacgcc cattcaggca
    39121 ctggcccttc cgctgacgct ggcgggtcgt gacgtagccg ggcaggcgca aaccggtacc
    39181 gggaaaacga tggcgtttct tacgtcaacg tttcattgtc ttctctctca tcccgcgatt
    39241 gccgatcgca aggtgaatca gccgcgtgcc ttaattatgg caccgacgcg tgaacttgcc
    39301 gtgcagatcc atgccgatgc ggaaccgctg gcggaagcta ccggcctgaa gctgggtctg
    39361 gcttacggtg gtgatggtta cgacaaacag ctgaaagtgc tggaaagcgg cgttgacatt
    39421 ctgattggca ccacggggcg tttaattgac tacgccaagc agaaccacat taacctcggt
    39481 gccattcagg tagtggtact ggacgaagcc gatcgcatgt acgatctggg ctttattaaa
    39541 gatatccgct ggctgttccg ccgtatgccg cctgtaaacc agcgcctcaa catgctgttc
    39601 tccgccacgc tttcgtaccg ggtacgtgaa ctggcgttcg agcagatgaa caatgccgaa
    39661 tatattgaag tggaaccgga acagaaaacg ggccaccgta ttaaagaaga gcttttctac
    39721 ccttctaacg aagaaaaaat gcgtttgctg caaacgctga tcgaagaaga gtggccagac
    39781 agagcgatta ttttcgccaa taccaaacac cgttgtgaag agatctgggg ccacctggcg
    39841 gcagatggtc atcgtgtcgg tttgttgaca ggcgatgtcg cgcagaaaaa acgtctgcgt
    39901 attcttgatg aatttacccg tggcgatctg gatattctgg ttgccaccga cgttgccgcg
    39961 cgtggtttgc atattccggc agtgacgcac gtctttaact acgatttacc cgatgactgt
    40021 gaagattacg ttcaccgtat tggtcgtaca ggtcgcgcag gcgcaagcgg tcactctatt
    40081 agcctggcgt gtgaagagta tgcattgaat ttgcctgcta ttgagaccta tattggtcac
    40141 tcaattccgg taagcaaata caatccggac gcattgatga ccgatctgcc aaaaccgctg
    40201 cgcctcacgc gcccgcgcac aggcaatggt ccgcgtcgta ctggcgctcc gcgtaatcgt
    40261 cgtcgttcag gttaaataaa aaatgtcata ctcaaaatca ttcaaactgt atcaagatgg
    40321 caagagaatg aatcccgatg agcttactgt agtaagtgat tcaggtgaaa gaatgcagcc
    40381 aacacagaga cagattgaag gatgaagagt atgggttcca cctcatcgct gtatgcagcc
    40441 attgatctcg gttcgaatag ttttcatatg ctggttgtgc gcgaggtggc tggaagcatc
    40501 cagacgctga cgcgaataaa acgcaaagtg cgtctggctg ctggcctgaa tagcgaaaat
    40561 gccctctcca atgaagcaat ggagcgcggt tggcaatgtt tgcgcctgtt tgctgaacgt
    40621 ctgcaagata ttcctccctc gcaaattcgc gttgtcgcta cggcgacgtt acgccttgcc
    40681 gtcaatgcgg gtgattttat tgccaaagcg caggaaatcc tcggttgtcc ggtacaggtg
    40741 atcagcggtg aagaggaagc acgtctgatt tatcagggtg ttgctcacac cactggcggt
    40801 gccgatcagc gcctggtagt ggatatcggc ggtgccagta ctgaactggt aaccggcacg
    40861 ggtgcacaaa ccacctcgtt gttcagcctg tcgatgggct gcgtcacctg gctggaacgc
    40921 tattttgccg atcgtaatct ggggcaggaa aattttgatg ctgcagaaaa agcggcacgc
    40981 gaagtgttac gtccggttgc cgatgaatta cggtatcacg gctggaaagt gtgcgttggt
    41041 gcttccggca ccgtgcaggc gttacaggaa atcatgatgg cacaggggat ggatgaacgc
    41101 attaccctgg aaaagttgca gcaattgaaa cagcgagcca ttcattgcgg tcggctggag
    41161 gaactggaga ttgacgggct gacgctggaa cgtgcgttag tgttcccgag tggtctggcg
    41221 atcctgatcg ccatttttac cgaactgaat attcagtgta tgaccctggc gggcggtgcg
    41281 ctgcgtgaag gcctggtcta cggcatgttg catctggccg tcgagcagga tattcgcagc
    41341 cgtacgctgc gtaatattca acgccgcttt atgatcgata ttgatcaggc acagcgcgta
    41401 gccaaagttg cggctaactt cttcgatcag gtggaaaacg aatggcatct tgaagcaata
    41461 agccgcgatt tgctcatcag cgcctgtcag cttcatgaaa tcggcctgag cgttgacttc
    41521 aaacaagcgc cgcaacacgc tgcttatctg gtgcgtaatc tggatcttcc cggttttacc
    41581 cccgcacaga aaaaattgct ggcgacgcta ctgctcaacc agactaatcc ggtcgatctc
    41641 tcatcgctgc atcagcaaaa tgccgtaccg ccgcgcgtcg cagaacaact ctgccgttta
    41701 ctacgcctgg ccatcatttt tgccagccgt cgccgtgacg atctcgtgcc agagatgaca
    41761 ttacaggcta accatgaact gttgaccttg acgcttccgc aaggttggct aacccaacat
    41821 ccgctgggta aagagattat tgctcaggaa agccagtggc agagctatgt ccactggccg
    41881 ctggaagtgc attaatgagt aagtgccgga tgcgatgctg acgcatcttt tccggccttg
    41941 attatttccc tcgttttgcc gccatcatcg ctttcagatt cgccagatgg ctttgccctt
    42001 tctgcatccg ttcttcggcg ctgaccactt tgcgctcctg ttcccaaatc agatcatcct
    42061 gcggcagctc cagcaaaaag cggctcggct ccgggcgcac cagttcgccg tactgacggc
    42121 gttctttaca cagcgtaaag gtcaattcct tctgggcgcg ggtaatgccg acataggcca
    42181 gccgccgctc ctcatcgata ttatcttcat cgatactgct ctggtgcggc aaaaaccctt
    42241 cttccatacc gaccatgtag acataaggaa actccagtcc ttttgacgcg tggagagtca
    42301 tcagttgcac ctgatccagc tcttcttcac tctcaccacg ctccatcatg tcgcgcaaag
    42361 taaagcgcgt caccacctgg gtgagcgtca tcggctcatc cagttcgctg ccttccagca
    42421 tctccgtcat ccagctaaac agttggttga cgttcttcat gcgcatttcg gcggctttcg
    42481 ggctgggcga tgtttcgtac agccaggatt cataatccat gccatggatc agatcacgca
    42541 ccgcagcaat cggctcccgc tccgccaggc gctggatttc tgccaaccag tgagtgaagc
    42601 gggtcaatgc ttcataacca cgtccgctaa gcgtctgact caggcccata tcaaagctgg
    42661 cggtaaacat gcttttattg cgcgtcatcg cccactcacc cagctttttc agcgtagccg
    42721 ggccaatctc tcgcttcggc gtgttaacga tacgcagaaa tgcgctgtca tcgtccgggt
    42781 tagtcagcac gcgcagataa gccagcaagt ccttgatttc aggacgagag aaaaacgacg
    42841 taccaccgga tattttgtac gggatgcggt tttgcatcag gaatttttca aatacccgcg
    42901 actgatggtt gccgcgataa agaatggcgt aatctttgta ctgcgtttta ttgacgaagt
    42961 gatgggcgat cagctcgcca gtaacgcgct cggcttcatg ttcctcgtta ttcgcgctta
    43021 aaacttttag ctctgtgccg taacccagtt cggagaacag acgcttttca aagacgtgcg
    43081 ggttattggc gatcaggatg ttcgccgctt tcagaatacg cccggaagag cgatagttct
    43141 gctcaagctt aatcaccttc agcgccggaa aatcctgact cagcagcacc aggttttgcg
    43201 gacgtgcacc gcgccaggag tagatcgact ggtcatcgtc acccaccacg gtaaagcgcg
    43261 cgcggctgcc caccagcagt ttcaccagtt catactggct ggtgttggtg tcctgatact
    43321 catccaccag caggtagcga attttgttct gccagcgttc gcgtacttct tcattgcgtt
    43381 gcagcagcaa cgtcggcaat aaaatcaggt catcgaagtc gagaacgtta caggctttca
    43441 ggtgtgcatc atacagccca taacaatggg caaaaatacg atcccgctcg ccctttgcct
    43501 ctgccgccgc ctgcgccgga gttttgagat cgttcttcca gttagagatg gtcgaaatca
    43561 gttgttgcag gagaactttg tcatcttcaa tcagcccctc ggtcaactct ttaagcaaag
    43621 caagctgatc ggtatcgtca aataacgaga agttcgcttt catcccaagc gccgcatact
    43681 cgcgtttgat gatatccagc cccaacgtat ggaaagtgga gatcatcagc ccacgcgcct
    43741 ctttacgccc cagcgtctgc cctacacgct ctttcatctc gcgcgctgct ttattagtaa
    43801 aggtcaccgc cgcaatgtgc cgcgcctgat aaccgcaacc gcggatcaga tgggcgattt
    43861 tattggtgat aacacgagtt ttaccggaac ccgcgcccgc cagcaccagg caggggccgg
    43921 taacgaattc gacagcttgt tgttggccgg ggtttagacg cataggtgta ttgctcaatc
    43981 ttcgaacggg gggaggattg tagcagaatc gcatttgaat gtgaccgcgt gtaagccgct
    44041 aaccggttgg ctgcggatgt ttaaatcaga gagagaagac cgaaatcgga gttcatcgtg
    44101 aatccattag acttagaaaa tatcgggtcg taacgttgtg ttattacttt ttcagagttt
    44161 actaaggaga ggtttactcg tggcgattaa tttcagccct aaggttggtg aaatactgga
    44221 atgcaacttt ggtaactatc ctgttagcca aaatggaccg ttcagcacga cgtattacga
    44281 tggccgcatt cctcctgaaa tgataaaaaa caggttagtt gtcgtgctca acggaaaaat
    44341 taatggcaac gcattcatcg tcgttccatt atcaaccact cgcgaccatg acaagttaaa
    44401 acgtggaatg catgttgaaa tagccagtaa tgttatcaat gacctgcaat tctttgatca
    44461 gcagattcgt tgggccaaaa ccgacctggt acaacaagtc agtcgaaaca gattaaacag
    44521 agcaagaact tacagagggt atcttaatca atgtttgcca catgagttgg ttgcagacat
    44581 acagagggcg gtcatcaagt caatcaatgc catatcgctc ataaattgac aataaaatca
    44641 ccatccccga taatgacttt gtagcccata gaggccctaa gttttctccc cttgagggac
    44701 ctgcgaagcc cggattttcc gggcttttgc catatatgta ccagcccctc cctgactgac
    44761 aattacgtta gcatcggcgg atcatgctaa aatcgccgcc tgacaattat tcactcaagg
    44821 cacgatcatg gcaaaaacag cagcagcact gcatatcctt gtaaaagaag agaaactggc
    44881 tctggatctt ctcgagcaga ttaagaacgg ggccgatttt ggcaagctgg cgaagaaaca
    44941 ctccatttgc ccatcaggca aacgcggcgg tgatttaggt gaattccgcc agggtcagat
    45001 ggttccggcg ttcgataaag ttgtgttctc ttgtccggta ctggagccaa ccggcccgct
    45061 gcacacccag ttcggttacc acatcattaa ggtgctgtac cgtaactaat agcaaggcct
    45121 tctccgggag aaggccttga actctgaatc gctattaacc cgcaacagca atacgtttca
    45181 tatcagtcat gtagccgcgc agtttcttac ctacctgctc aatcgcatgg ctgcgaatcg
    45241 cttcgttcaa atcacgcagt tgcgcgttat ctaccgcacc ttccggaata gctttaccca
    45301 aatcgcccgg ttgcagctct gccataaacg gtttcagcaa cggcacacaa gcgtaagaga
    45361 acagatagtt accgtactca gcggtatcag agataaccac gttcatttcg tacagacgct
    45421 tacgggcgat ggtgttggca atcagcggca gttcgtgcag tgattcatag taagcagatt
    45481 cttcgatgat gccggaatca accatggttt caaacgccag ctcaacgccc gctttcacca
    45541 tcgcgatcat cagtacgcct ttatcgaagt actcctgctc gccgattttg ccttcatact
    45601 gcggcgcggt ttcaaacgcg gttttgccgg tctcttcacg ccaggtcagc agtttcttat
    45661 catcgttggc ccagtccgcc atcatgccgg aggagaattc gccggagatg atgtcgtcca
    45721 tatgtttctg gaacagcggt gccatgatct ctttcagctg ttctgacaac gcgtaagcgc
    45781 gcagtttcgc cgggttagag agacggtcca tcatcagggt gatgccgccc tgtttcagcg
    45841 cttcggtgat ggtttcccag ccgaactgaa tcagtttttc tgcgtatgcc ggatcggtgc
    45901 cttcttccac cagcttgtcg aagcacagca gagagccggc ctgcaacata ccgcacagaa
    45961 tggtttgctc gcccatcagg tcagatttca cttccgcaac gaaagaggat tccagcacgc
    46021 ccgcacgatg accaccggtt gccgccgccc atgctttagc gatcgccatg ccttcgcctt
    46081 tcggatcgtt ttccgggtga acggcaatca gcgtcggtac gccgaatcca cgtttgtact
    46141 cttcacgtac ttcggtgcct gggcatttcg gcgcaaccat tactacggtg atgtctttac
    46201 ggatctgctc gcccacttcg acgatgttga aaccatgcga gtaacccagc gccgcgccgt
    46261 ctttcatcag tggctgtacg gtgtgcacta catcagagtg ctgcttgtcc ggcgtcaggt
    46321 taaccaccag atccgcctgt gggatcagtt cttcgtaagt acccacttta aaaccatttt
    46381 cggtcgcttt acgccaggac gcgcgcttct cggcaatcgc ttctttacgc agagcgtagg
    46441 agatatcgag accagaatca cgcatgttca ggccctggtt cagaccctgt gcgccacagc
    46501 cgacgatgac cactttttta ccctgaaggt agctcgcgcc atcggcgaat tcgtcgcggc
    46561 ccataaagcg acatttgccc aactgtgcca gttgctggcg caggttcagt gtattgaagt
    46621 agttagccat ggtgattcct cgtgatgttg tgcttcttat tgttcggttc gcgtttgcaa
    46681 gattgaattc actatatgac aggaaattta ttgcggaaat tgatatattc acaacatcat
    46741 attgcaattt ttgcaacgtc aacatcgagg gctgtccctg tggatttacg cgatctgaaa
    46801 accttcctgc atctggcgga aagccgccat tttggccgca gcgcgcgggc gatgcacgtt
    46861 agcccatcca cgctctcacg gcagattcag cgcctggaag aagatctcgg tcagccgctg
    46921 tttgtgcgcg ataaccgcac ggtgacgctg actgaagcgg gcgaagaact gcgcgttttc
    46981 gcccagcaaa cgctgttgca gtatcagcag ctgcgccaca ccatcgatca gcaagggccg
    47041 tcgctctctg gcgaattaca tatcttctgc tcggtgaccg ctgcctacag ccatctgccg
    47101 ccgattctgg atcgcttccg cgcggaacac ccgtcggtgg agattaaact cactactggt
    47161 gatgcggcag atgcaatgga aaaagtggtc acgggagaag cggatctggc gattgccggg
    47221 aaaccggaga cgctacccgg cgctgtagcg ttttcgatgc tggagaatct ggcggtagtg
    47281 ctgattgcgc ccgcgctgcc ctgcccggtg cgtaatcagg tctcggtaga gaagccggac
    47341 tggtcaacgg taccgtttat catggccgat caggggccag tacgccgccg cattgaactg
    47401 tggtttcgac gcaataaaat cagcaaccca atgatttacg ccacggttgg cgggcatgaa
    47461 gcgatggtat cgatggtggc gctcggctgt ggtgtggcgc tgttgccgga agtggtgctg
    47521 gaaaacagcc cggagcctgt acgcaaccgg gtaatgattt tagatcgcag cgatgagaaa
    47581 acgccgtttg aactgggcgt gtgtgcacaa aaaaagcggc tgcatgagcc gctgattgag
    47641 gcgttctgga ggattttgcc gaaccacaaa tgacgttgtc gcgcgagtag gcctgataag
    47701 cgaagcgcta tcaggcattt ttccctaacc cgccaaaaag aacctgaacg ccgggttatt
    47761 ggtttcgtcg tggcaatcgt agcccagctc attcagccgg gtttcgaaat ccggttcatg
    47821 gtcgccaagt tcgaacgccg ccagtacgcg cccgtagtcg gtgccatggc tgcgatagtg
    47881 gaacaaagaa atgttccagt gcgtacccag cgtgttgagg aagcgcagca gcgcgcccgg
    47941 tgattccggg aattcgaagc tgtagaggcg ttcctgcaac ggatgtgacg gacgcccgcc
    48001 gaccatatag cgcacatgca acttcgccat ttcgtcgtcg gagagatcca ccacgctgta
    48061 gccgccgtcg ttgagcatct gcaaaatttc tttgcgctct tcgaggccgc ggctcaggcg
    48121 cacgccgaca aagatgcagg cgtttttggc atcggcaaaa cggtagttga actcggtgac
    48181 cgatcgcccg ccaagcagtt ggcagaattt gaggaagctg cctttttctt ccggaatggt
    48241 caccgccaac aacgcttcac gctgttcgcc cagttcgcag cgttccgaga cgtagcgcag
    48301 gccgtggaag ttcacgttgg caccggaaag aatatgcgcc agccgttcgc cgcgaatgtt
    48361 gtgctgggcg atatattttt tcattcccgc cagcgccagc gcgccagagg gttccgccac
    48421 cgcgcgcaca tcttcgaata aatccttcat cgccgcacag atcgcatcac tatcgacggt
    48481 gatgatatcg tcgagatact cctggcacaa acggaaggtt tcgtcgccga tgcgttttac
    48541 cgcaacgcct tcagcaaaca gccccacgcg cggcagatca accggatgac ccgcatccag
    48601 cgctgctttc aggcaggcgg agtcttccgc ttctacggcg attactttga tttgcggcat
    48661 cagttgtttg atcagcaccg ccacgccagc agccagaccg ccgccgccga ctggcacaaa
    48721 tacgcggtcg agatgggcgt cctgctggag cagttccagc gccagcgtgc cttgcccggc
    48781 aatcaccatc ggatggtcga acggcggcac ccaggtgaac ccctgctgct gtgacagttc
    48841 gatcgctttg gctttcgctt catcaaagtt cgcgccgtgg agcagcactt cgccgccgaa
    48901 gccgcgcacc gcgtcgactt tgatgtcggc ggtggcggtt ggcataacga tcagggcctt
    48961 cacgcctaac cgcgcagaag aaaacgcgac gccctgcgcg tggttacccg cagaagcagt
    49021 gatcacgccg tgcgcttttt gttcttccgt caggcccgcc atcatggcgt atgcgccgcg
    49081 cagcttaaag ctgtgcactg gctggcgatc ttcgcgcttc accagaatca cgttatcaag
    49141 acgagacgag agcttttcca ttttttgtag cggcgtgacc tgcgccgctt cgtagaccgg
    49201 cgcgcgcagc accgctctta aatattcagc accttccggg gcaccggaca ggggttgcga
    49261 gtcagccatt attaaccccc cagtttcgat ttatcgcgca ccgcgccttt gtcggcgctg
    49321 gttgccaggc tggcataagc acgcagggca aaggagatct gacgttcacg atttttcggc
    49381 gtccaggctt tgtcaccccg agcttcctgc gcttcacgac gcgccgccag ttcggcatcg
    49441 cttacctgta actgaatgcc acggttcgga atgtcgatgg cgatcaggtc gccgtcttca
    49501 atcaggccaa tgctgccgcc gcttgccgct tccggtgaga cgtggccgat agaaaggcca
    49561 gaggtgccgc cagagaaacg accgtcggtg atcagcgcac aggctttacc gagccccatt
    49621 gatttcagga agctggttgg gtagagcatt tcctgcatcc ccggaccacc ttttgggcct
    49681 tcatagcgaa ttactaccac atctccggcg acaactttac cgccgagaat cgcttctacc
    49741 gcgtcgtcct ggctttcgta cactttcgcc gggccggtga atttgaggat gctgtcatcg
    49801 acgcccgcag ttttaacgat gcagccgttt tccgcgaaat taccgtagag caccgccagg
    49861 ccgccgtctt tgctataggc gtgttccagc gagcggatac agccattggc gcgatcgtcg
    49921 tccagcgtat cccaacggca atcttgcgag aatgcctggg tggtacggat acccgcaggg
    49981 ccggcgcgga acatattttt taccgcgtca tcctgggtca gcataacgtc gtattgttcc
    50041 agcgtttgcg gcaacgtcag gccaagtacg tttttcacat cacggttcag taaccccgca
    50101 cgatccagct cgccgagaat accgataaca ccaccagcac ggtgaacatc ttccatatgg
    50161 tatttttggg tgctcggcgc gactttgcat aactgcggaa ccttgcggga aagcttatcg
    50221 atatcactca tggtgaagtc gatttccgct tcctgcgccg ccgccagcag gtgaagtacg
    50281 gtgttagtcg atccacccat cgcgatatcc agcgtcatgg cattttcaaa cgccgctttg
    50341 ctagcaatat tgcggggcag tgcactttcg tcgttttgct cgtagtaacg tttggtcaat
    50401 tcaacaatgc gtttaccagc attgaggaac agctgcttac ggtcagagtg ggttgccagc
    50461 agcgagccgt tgcccggctg cgacagaccc agcgcttcgg tcaggcagtt cattgagtta
    50521 gcggtaaaca tcccggagca ggaaccgcag gttgggcatg cggaacgttc aacctgatcg
    50581 ctctgggagt cagatacttt cgggtctgcg ccctggatca tcgcatcaac cagatcgagc
    50641 ttgatgatct gatcggacag tttggttttc ccggcctcca tcgggccgcc ggaaacaaag
    50701 atcaccggaa tattcagacg cagggaagcc atcagcatcc ccggggtgat tttgtcacag
    50761 ttagagatgc agaccatggc gtcggcgcag tgggcgttga ccatatactc aacggaatca
    50821 gcgatcagtt cgcgagatgg cagtgaataa agcatccccc cgtggcccat ggcaatccca
    50881 tcatccaccg caatggtgtt gaactctttg gcaacgccgc cagccgcttc aatttgttcg
    50941 gcgaccagtt taccgagatc gcgcagatgg acgtgacccg gtacaaattg ggtgaacgag
    51001 ttcacaaccg cgataatcgg cttaccgaaa tcggcgtcgg tcattccggt ggcgcgccac
    51061 agcgcacgag cacccgccat attacgacca tgagtggtgg tggcggaacg gtacttaggc
    51121 atactttatt tactcccagt gtctgtctcg taaatgggac ggtgcgtgcc gtcccatttt
    51181 ttgtatttat tgattaactt gatctaacca gccccattta tcttcggttt cgccggtaaa
    51241 gaggccgaag aaggcttgct gaatgcgttt ggtgaccggg ccacaacggc cttcgccaac
    51301 ctgaataccg tctacactgc gcactggcgt gatttctgcc gccgtaccgg acataaagac
    51361 ttcatccgcc aggtacaagg attcgcgcga cagcacctgc tcacgcactt caattcccag
    51421 ctctttcgcc agtttgatga tggcatcacg ggtaataccc ggcagcgcgg aggaggtgaa
    51481 cggtggggtg aacagcacac catctttcac ttcaaacagg ttttcgcctg cgccttcaga
    51541 gatataaccg ttcacatcca gcgcgatacc ttcctgataa ccgtggcggc gcgcttcgct
    51601 acccaccagc agggaagaga gatagttacc accggctttt gccgcagttg ggatggtgtt
    51661 tggtgctgcg cggttccagg aggaaaccat cgcatcgatc ccctgctcca gcgcttctgc
    51721 gcccagatac gctccccacg ggaaagcggc gataatcaca tcggttgagt atcccgctgg
    51781 cgggtttacg cccatgccaa catcaccaac gaagatcagc ggacggatat aggcgctggt
    51841 gagattgttt ttgcggatca cgtcacggca agcttccatc agctcatcaa tgctctgcga
    51901 aaccgggaag cgatagattt tggcggagtc atgcagacgc tgcatatgct cacgatggcg
    51961 gaatacaacc ggtcctttgt gcgagtcgta gcaacggatg ccttcaaaaa ccgaggtgcc
    52021 atagtgcagc gcgtgcgaca tcacatgcac cttcgcgtct tcccagcgaa ccatctcccc
    52081 attgaaccaa atgtaatcag ctttcttcgt ggtcattttt atattccttt tgcgctcagg
    52141 cgcggatttg ttgtgatgtg gttgtgctct ggcagatggc aacgtgtgcg acgtccacca
    52201 gtttatttaa ctgactaaac agtaagtcga ccgaccgtgg gctggcaacg gtcaattcga
    52261 tatttatatt ttgtgcatcg ctggcggcgg ccatattcat tgagcagacg tggaaaccac
    52321 gatgacgcac cacgcgtaaa acacgttcta aggtttccgg attgaagcga gccgatacat
    52381 tgacctgatg ttgcatcatg ataatttctc caacatttct gaattactgg caccaggcgg
    52441 caccagcggc cagacgttct caagttcgtc gattgagaca tgaagcaggt atggcccatc
    52501 actgttcagc atggtgtcga gtgccgcttc aacctggtct ttacgggtga tgtgttggcc
    52561 agggatgccg aaggcgctgg ctaacttgag gaaatcgggg ttatcggtaa gggtggtttc
    52621 gctgtatcgt tcctggaaaa acagttgctg ccattgtcga accatcccta accgttggtt
    52681 atcgagtaag acgattttca acggtaactg cttgcgtttt acggtgccca gctcttgtac
    52741 attcatcatg aaagagccgt caccggagat acagacgacg gtatcgtttg gtcgcgcgac
    52801 ttgcgcgcca accgccgccg gtaaaccaaa acccatggtg cctaagccgc tggaggtaat
    52861 gaaattttcc gggcgagtgt gtgcgatgtg ctgcgcggcc cacatctgat gctgccccac
    52921 atctgtggtc acaacgcaat ccgcaggttt acgatccgac agttgtttta acaacaacgg
    52981 cgcgtagata gcgtcaccgg gatggtcgta acgccaggca tgttcatcac gcagctgcgc
    53041 gcagtattgc tgccagtcat tgatatttaa tggctgctgt aatgctggta acagcgcatt
    53101 taaatcaccc tgtaatgcca catgtgcctg acgcagcttg ttcatttctg ccgggtcgat
    53161 atccatatgg ataacactgg cgtgtggtgc gaaggtgttc aatttgccgg tcacccggtc
    53221 atcaaaacgt gcacccacgg cgatcagcaa gtcgcactcc tgcaccgcga agttcgccgc
    53281 tttggtgcca tgcattccca gcatgcccag atagtacgga taatctgctt caactgcgcc
    53341 cagccctttc agcgtgcagg tggcaggcat ttttgtggta gcgagaaatt ctcgtaaagc
    53401 aggaactgcc tgcgccatac ccacgccacc accaacgtac agcatcggtt tttgcgcttt
    53461 tgccagcatc tggcgcgctt gctcaacttc ggcatgtggg aaagtcactt cgttttcaac
    53521 ggtggtgaac cacggttcca ggtcgccgct ggctaactgg atatcttttg ggatatcgac
    53581 cagaaccgga ccaggacgac ctgagctggc aacgtcgaat gcttcagcca taatgcgcgg
    53641 caactcttcc agcgactgca ccagaaagct gtgcttggta caggctaacg acaatcccag
    53701 gacatccact tcctgaaatg cgtcagtacc gataaacggt gcggacactt gaccggtgat
    53761 ggcaacaaca gggatagaat ctaacagtgc gtccgcaagc ccggttatca ggttggttgc
    53821 gcccggacca gacgtggcga tacatacgcc agttttgccg gtagcacggg cataaccgat
    53881 agccgccatt gccgcaccct gctcatgtcg gcacagcaag tgctccacgc cgccgtcata
    53941 caatgcatcg taaaccggca taattgcgcc acccggataa ccgaaaacgg tgttcacacc
    54001 ctgtgcccgc aacgcatgta ccacccactg tgcgccattc atagttagtt ccccgtcctg
    54061 aatcttggga aacagaattt tgtgctgtta ttcattgtct gctcctcggt tatgttttta
    54121 aggtcaaaaa aaaaccccgg acctttcggt gcgggggtct tagttcgtta aggcttgatc
    54181 tctaagcctt tcctcgtcca agtgcagccc cgcacggtgg gataataatc accaccacgc
    54241 taatcacgac caggctaatc actcgtagaa gggctgtcat tttgtctttt cttgcatctt
    54301 gttcgaagga atgcctaaag agttaccata gattttgcga atagcacaag atattttttg
    54361 gccgtttttt atgatggctt taataatttc aatagaaatt gttgtttttt aaaaagaaaa
    54421 tagtgagtga aaaaaattca atttcagtac gaatgttgtt ccctccctcg tagttcatcc
    54481 tttgtccgct gcatttttgc gagcatcatt ccaccgccag aacaactcaa cttcacttgc
    54541 acaaataaac aggaatcagc cactaaaatt cacgaattag cgggctacag cagcgatatc
    54601 ttatgcacat aatggtgact caaggagggc ttatgtcact gtcaattgtt catacccgcg
    54661 cagccctggg agtaaatgcg cccccaatca ctgttgaggt acatatcagt aaaggtttgc
    54721 ccggcttaac gatggtgggc ttaccagaaa caacggtaaa agaagctcgc gatcgcgtgc
    54781 gcagcgccat tatcaatagc ggatatgaat atccggcgaa aaaaatcacc attaaccttg
    54841 ctccagccga tctaccaaaa gaagggggac gatatgattt acctatcgcc attgcgttgc
    54901 tggcggcctc agaacagctt acagccaata agttagatga atatgaatta gtcggagaac
    54961 tggcgcttac aggcgctctg cgtggcgttc ccggtgcaat ctccagtgca actgaagcta
    55021 ttaagtcggg cagaaaaatt atcgtcgcga aagataacga agatgaagtg gggctaatta
    55081 acggtgaagg atgcctggta gccgatcatc tgcaagctgt ctgtgcgttt ctggaaggta
    55141 agcacgctct cgaacgcccg aaaccaactg atgcagtatc ccgggcgcta caacatgatc
    55201 tcagtgatgt tgtcggtcag gagcaaggaa agcgaggact ggaaattacc gccgctggcg
    55261 ggcacaacct tttactgatt gggccgccgg gaacaggtaa aacaatgctc gccagccgta
    55321 ttaatggtct tttgccagat ttaagcaatg aagaggcact ggagagcgct gcgatattaa
    55381 gtctggtaaa tgctgaatca gtacaaaaac aatggcggca gcgcccgttc cgctcacctc
    55441 atcacagtgc gtcgttaacg gcgatggtgg gcggtggtgc aattccaggg ccaggtgaaa
    55501 tttcgttggc gcataacggc gtgctttttc ttgatgagct acctgaattt gaacggcgta
    55561 cactggatgc tttgcgagag ccgattgaat ccgggcagat ccatctttca cgcacacgag
    55621 caaaaataac ctatccagcc cgtttccagc ttgttgcggc gatgaatccc agccctaccg
    55681 gacattatca gggaaaccat aaccgctgca cgccagaaca gacattacgt tatctcaacc
    55741 ggctctcggg gccctttctc gaccgcttcg atctctcact ggagatccca ttaccacccc
    55801 ccggcatttt gagtaaaacg gtagtgccgg gagaaagcag caccaccgtt aaacaacgtg
    55861 taatggctgc cagagagcgc caatttaagc ggcagaataa gttgaacgcc tggctggata
    55921 gtccggaaat acgcaaattc tgcaagcttg agagcgaaga tgcgcagtgg ctggaagaaa
    55981 cgctgatcca tctggggtta tcgattcgtg cctggcagcg gttattgaaa gttgcacgaa
    56041 ccattgctga tattgatcag tctgacatta tcacacgtca gcatttgcag gaggcagtta
    56101 gctatcgtgc gattgaccgt ttgctcatcc atctgcaaaa actactgaca taaaaaaagg
    56161 gcatttcgcc ctttttatta atcgtcagaa tcggtgtagt cttcagcacc ttcaacctgc
    56221 ggtttaccgc cggaaagggt gtgaaaacgt tttggacgct tgatacgcgt catatacttg
    56281 gaccacacgc gttctgcttc tgtcactggc tcacgttcgc cacggcatac tgctacgaag
    56341 agtttctctt cctcggtaac cggctcgcgt ttgccaagat ccaactcatt gaaggcataa
    56401 ccatgacgct caagcagttg tgcctctttg atggtgaaat caccatggcg agagaatcca
    56461 cgtggataat gtttattgtc gaaatatcga ttagtcgtcg taaagctttc cgccatcctg
    56521 cacgctccta attctttgac cgagctagtt atggcgcgga gtattagtta cgcttgacag
    56581 agtgtaaaac aaaacattta aatcataacg acaaataatt ttgcggagag cactgtggat
    56641 acggaattgt taaaaacttt cctggaagtt agccgaacgc gtcactttgg tcgagcggct
    56701 gaatcgctct atctgaccca gtcagcagtg agctttcgaa tcagacaact ggaaaatcaa
    56761 ctgggtgtga accttttcac ccgccacaga aacaatatcc gtttaaccgc tgccggtgaa
    56821 aaactactgc cttatgcaga aacgctcatg agcacctggc aggccgcccg taaggaggtg
    56881 gcgcatacct cacgacataa cgagttttct atcggtgcca gcgcctcatt gtgggaatgt
    56941 atgcttaatc agtggctggg acgtttgtat caaaatcagg atgcccatac aggcttacag
    57001 ttcgaagcgc gaattgccca acggcagtct ctggtaaaac agctgcatga acgccagctt
    57061 gatcttctta ttaccactga agcgcccaaa atggacgaat ttagtagtca gttgctgggg
    57121 tatttcactt tagcgcttta taccagtgcc ccttctaaac taaagggaga tcttaactat
    57181 ctgcgactgg agtgggggcc agattttcaa cagcatgagg caggtttgat cggtgctgac
    57241 gaagtgccca ttctgacaac cagttctgct gaactggcac agcaacagat tgcgatgctt
    57301 aatggttgca cctggctacc cgtcagctgg gcgcgtaaaa aaggcggcct gcataccgtt
    57361 gtcgatagca caacactttc acggccgctt tatgccatat ggctgcaaaa tagcgataaa
    57421 aatacgttga ttcgcgatct attgaaaatt aacgtgctgg atgaagtgta ttaatctgaa
    57481 tggctggcaa ggatgccggt agaaggattt acttcagaga gggttatttc agataaaaaa
    57541 aatccttagc tttcgctaag gatgatttct ggcaggggcg gagagactcg aactcccaac
    57601 acccggtttt ggagaccggt gctctaccaa ttgaactacg cccctaatta g
//
LOCUS       DAZFAB010000020        56108 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZFAB010000020 DAZFAB010000000
VERSION     DAZFAB010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 56108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56108
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.020"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            181..2322
                     /locus_tag="ACHJWA_001413"
     CDS             181..2322
                     /locus_tag="ACHJWA_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HFO4308045.1"
                     /translation="MAKFTPSFSGIKGRALFSLLFAAPMIHATDTATTKDGETITVTA
                     DANTATEATDGYQPLSTSTATLTDMPMLDIPQVVNTVSDQVLENQNATTLDEALYNVS
                     NVVQTNTLGGTQDAFVRRGFGANRDGSIMTNGLRTVLPRSFNAATERVEVLKGPASTL
                     YGILDPGGLINVVTKRPEKTFHGSVSATSSSFGGGTGQLDITGPIEGTQLAYRLTGEV
                     QDEDYWRNFGKERSTFIAPSLTWFGDNATVTMLYSHRDYKTPFDRGTIFDLTTKQPVN
                     VDRKIRFDEPFNITDGQSDLAQLNAEYHLNSQWTARFDYSYSQDKYSDNQARVTAYDA
                     TTGTLTRRVDATQGSTQRMHSTRADLQGNVDIAGFYNEILGGVSYEYYDLLRTDMIRC
                     KNAKDFNIYNPVYGNTSKCTTVSASDSDQTIKQESYSAYAQDALYLTDNWIAVAGIRY
                     QYYTQYAGKGRPFNVNTDSRDEQWTPKLGLVYKLTPSVSLFANYSQTFMPQSSIASYI
                     GDLPPESSNAYEVGAKFELFDGITADIALFDIHKRNVLYTESIGDETIAKTAGRVRSR
                     GVEVDLAGALTENINIIASYGYTDAKVLEDPDYAGKPLPNVPRHTGSLFLTYDIHNMP
                     GNNTLTFGGGGHGVSRRSATNGADYYLPGYFVADAFAAYKMKLQYPVTLQLNVKNLFD
                     KTYYTSSIATNNLGNQIGDPREVQFTVKMEF"
     gene            complement(2386..4278)
                     /gene="parE"
                     /locus_tag="ACHJWA_001414"
     CDS             complement(2386..4278)
                     /gene="parE"
                     /locus_tag="ACHJWA_001414"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFO4308046.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGNCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(4307..4888)
                     /gene="yqiA"
                     /locus_tag="ACHJWA_001415"
     CDS             complement(4307..4888)
                     /gene="yqiA"
                     /locus_tag="ACHJWA_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFO4308047.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(4888..5715)
                     /gene="cpdA"
                     /locus_tag="ACHJWA_001416"
     CDS             complement(4888..5715)
                     /gene="cpdA"
                     /locus_tag="ACHJWA_001416"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFO4308048.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(5740..6162)
                     /gene="yqiB"
                     /locus_tag="ACHJWA_001417"
     CDS             complement(5740..6162)
                     /gene="yqiB"
                     /locus_tag="ACHJWA_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFO4308049.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(6163..6792)
                     /gene="nudF"
                     /locus_tag="ACHJWA_001418"
     CDS             complement(6163..6792)
                     /gene="nudF"
                     /locus_tag="ACHJWA_001418"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFO4308050.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            6997..8478
                     /gene="tolC"
                     /locus_tag="ACHJWA_001419"
     CDS             6997..8478
                     /gene="tolC"
                     /locus_tag="ACHJWA_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFO4308051.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTASS
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            8626..9297
                     /gene="ygiB"
                     /locus_tag="ACHJWA_001420"
     CDS             8626..9297
                     /gene="ygiB"
                     /locus_tag="ACHJWA_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFO4308052.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            9303..10463
                     /gene="ygiC"
                     /locus_tag="ACHJWA_001421"
     CDS             9303..10463
                     /gene="ygiC"
                     /locus_tag="ACHJWA_001421"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO4308053.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(10501..11289)
                     /gene="ygiD"
                     /locus_tag="ACHJWA_001422"
     CDS             complement(10501..11289)
                     /gene="ygiD"
                     /locus_tag="ACHJWA_001422"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFO4308054.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQTLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            11432..12205
                     /gene="zupT"
                     /locus_tag="ACHJWA_001423"
     CDS             11432..12205
                     /gene="zupT"
                     /locus_tag="ACHJWA_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFO4308055.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(12263..12433)
                     /gene="yqiD"
                     /locus_tag="ACHJWA_001424"
     CDS             complement(12263..12433)
                     /gene="yqiD"
                     /locus_tag="ACHJWA_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFO4308056.1"
                     /translation="MFIAWYWIVLIVLVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(12695..13348)
                     /gene="ribB"
                     /locus_tag="ACHJWA_001425"
     CDS             complement(12695..13348)
                     /gene="ribB"
                     /locus_tag="ACHJWA_001425"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFO4308057.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(13452..13600)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            13722..14021
                     /gene="ubiK"
                     /locus_tag="ACHJWA_001426"
     CDS             13722..14021
                     /gene="ubiK"
                     /locus_tag="ACHJWA_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311957.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFO4308058.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRSSELEARNNSVADLQSPPAIPPIDKAE"
     gene            complement(14056..14256)
                     /gene="glgS"
                     /locus_tag="ACHJWA_001427"
     CDS             complement(14056..14256)
                     /gene="glgS"
                     /locus_tag="ACHJWA_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFO4308059.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQTRELELEH"
     gene            14525..15139
                     /gene="yqiJ"
                     /locus_tag="ACHJWA_001428"
     CDS             14525..15139
                     /gene="yqiJ"
                     /locus_tag="ACHJWA_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFO4308060.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEIFALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHTCIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDHFGQIHYLLLEPEEGKIFTKGDKVLIICRLSATRYLAENNP"
     gene            15181..16842
                     /gene="yqiK"
                     /locus_tag="ACHJWA_001429"
     CDS             15181..16842
                     /gene="yqiK"
                     /locus_tag="ACHJWA_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFO4308061.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNAKIAAFEA
                     ERRREAEQTRILAERQIQETEIDREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGGTAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSLLTPTTPVAENVE"
     gene            17081..17140
                     /locus_tag="ACHJWA_001430"
     CDS             17081..17140
                     /locus_tag="ACHJWA_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO4308062.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            17457..17516
                     /gene="ibsE"
                     /locus_tag="ACHJWA_001431"
     CDS             17457..17516
                     /gene="ibsE"
                     /locus_tag="ACHJWA_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HFO4308063.1"
                     /translation="MMKLVIILVVLLLLSFPTY"
     gene            complement(17636..19069)
                     /gene="hldE"
                     /locus_tag="ACHJWA_001432"
     CDS             complement(17636..19069)
                     /gene="hldE"
                     /locus_tag="ACHJWA_001432"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFO4308064.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPSSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVVA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(19117..21957)
                     /gene="glnE"
                     /locus_tag="ACHJWA_001433"
     CDS             complement(19117..21957)
                     /gene="glnE"
                     /locus_tag="ACHJWA_001433"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFO4308065.1"
                     /translation="MKPLSSPLQQYWQTIVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SISAHPEWLTELESQPPQADEWQHYVAWLQEALINVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLLRITALLAGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSTEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVPEDCFTAERELVRASWQKWLVEE"
     gene            complement(21980..23281)
                     /gene="ygiF"
                     /locus_tag="ACHJWA_001434"
     CDS             complement(21980..23281)
                     /gene="ygiF"
                     /locus_tag="ACHJWA_001434"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFO4308066.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLHHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            23523..24143
                     /gene="ygiM"
                     /locus_tag="ACHJWA_001435"
     CDS             23523..24143
                     /gene="ygiM"
                     /locus_tag="ACHJWA_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFO4308067.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            24207..25445
                     /gene="cca"
                     /locus_tag="ACHJWA_001436"
     CDS             24207..25445
                     /gene="cca"
                     /locus_tag="ACHJWA_001436"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFO4308068.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETENALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFTTLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPE"
     gene            complement(25626..26447)
                     /gene="bacA"
                     /locus_tag="ACHJWA_001437"
     CDS             complement(25626..26447)
                     /gene="bacA"
                     /locus_tag="ACHJWA_001437"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO4308069.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTSGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(26538..26906)
                     /gene="folB"
                     /locus_tag="ACHJWA_001438"
     CDS             complement(26538..26906)
                     /gene="folB"
                     /locus_tag="ACHJWA_001438"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFO4308070.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DNVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            27012..27629
                     /gene="plsY"
                     /locus_tag="ACHJWA_001439"
     CDS             27012..27629
                     /gene="plsY"
                     /locus_tag="ACHJWA_001439"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFO4308071.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(27642..28574)
                     /gene="ttdR"
                     /locus_tag="ACHJWA_001440"
                     /gene_synonym="dan"
     CDS             complement(27642..28574)
                     /gene="ttdR"
                     /locus_tag="ACHJWA_001440"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFO4308072.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVYFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            28781..29692
                     /gene="ttdA"
                     /locus_tag="ACHJWA_001441"
     CDS             28781..29692
                     /gene="ttdA"
                     /locus_tag="ACHJWA_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFO4308073.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATIKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIVPDGDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            29689..30294
                     /gene="ttdB"
                     /locus_tag="ACHJWA_001442"
     CDS             29689..30294
                     /gene="ttdB"
                     /locus_tag="ACHJWA_001442"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFO4308074.1"
                     /translation="MKKILTTPIKAEDLQDIHVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPLPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            30343..31806
                     /gene="ttdT"
                     /locus_tag="ACHJWA_001443"
     CDS             30343..31806
                     /gene="ttdT"
                     /locus_tag="ACHJWA_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFO4308075.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(31849..32862)
                     /gene="tsaD"
                     /locus_tag="ACHJWA_001444"
     CDS             complement(31849..32862)
                     /gene="tsaD"
                     /locus_tag="ACHJWA_001444"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFO4308076.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            33100..33315
                     /gene="rpsU"
                     /locus_tag="ACHJWA_001445"
     CDS             33100..33315
                     /gene="rpsU"
                     /locus_tag="ACHJWA_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFO4308077.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            33426..35171
                     /gene="dnaG"
                     /locus_tag="ACHJWA_001446"
     CDS             33426..35171
                     /gene="dnaG"
                     /locus_tag="ACHJWA_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFO4308078.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            35366..37207
                     /gene="rpoD"
                     /locus_tag="ACHJWA_001447"
     CDS             35366..37207
                     /gene="rpoD"
                     /locus_tag="ACHJWA_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFO4308079.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(37286..37792)
                     /gene="mug"
                     /locus_tag="ACHJWA_001448"
     CDS             complement(37286..37792)
                     /gene="mug"
                     /locus_tag="ACHJWA_001448"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFO4308080.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDHQLKPQEAQHLLDYRCGVTKLVDRPTVQANEISKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLSIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            37917..37992
                     /locus_tag="ACHJWA_001449"
     tRNA            37917..37992
                     /locus_tag="ACHJWA_001449"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37950..37952,aa:Ile,seq:cat)
     gene            complement(38046..38810)
                     /gene="nfeF"
                     /locus_tag="ACHJWA_001450"
                     /gene_synonym="yqjH"
     CDS             complement(38046..38810)
                     /gene="nfeF"
                     /locus_tag="ACHJWA_001450"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFO4308081.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            39098..39721
                     /gene="nfeR"
                     /locus_tag="ACHJWA_001451"
                     /gene_synonym="yqjI"
     CDS             39098..39721
                     /gene="nfeR"
                     /locus_tag="ACHJWA_001451"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFO4308082.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLNLRVNQSDITDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(39875..41395)
                     /gene="aer"
                     /locus_tag="ACHJWA_001452"
     CDS             complement(39875..41395)
                     /gene="aer"
                     /locus_tag="ACHJWA_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFO4308083.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTGKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYILCALVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            41813..43192
                     /gene="ygjG"
                     /locus_tag="ACHJWA_001453"
     CDS             41813..43192
                     /gene="ygjG"
                     /locus_tag="ACHJWA_001453"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFO4308084.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(43234..43566)
                     /gene="ygjH"
                     /locus_tag="ACHJWA_001454"
     CDS             complement(43234..43566)
                     /gene="ygjH"
                     /locus_tag="ACHJWA_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFO4308085.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRIV"
     gene            43785..44768
                     /gene="ebgR"
                     /locus_tag="ACHJWA_001455"
     CDS             43785..44768
                     /gene="ebgR"
                     /locus_tag="ACHJWA_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFO4308086.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            44952..48044
                     /gene="ebgA"
                     /locus_tag="ACHJWA_001456"
     CDS             44952..48044
                     /gene="ebgA"
                     /locus_tag="ACHJWA_001456"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFO4308087.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKT
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAIFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            48041..48490
                     /gene="ebgC"
                     /locus_tag="ACHJWA_001457"
     CDS             48041..48490
                     /gene="ebgC"
                     /locus_tag="ACHJWA_001457"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFO4308088.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVDEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            48553..49986
                     /gene="ygjI"
                     /locus_tag="ACHJWA_001458"
     CDS             48553..49986
                     /gene="ygjI"
                     /locus_tag="ACHJWA_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO4308089.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            50120..51190
                     /gene="ygjJ"
                     /locus_tag="ACHJWA_001459"
     CDS             50120..51190
                     /gene="ygjJ"
                     /locus_tag="ACHJWA_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HFO4308090.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASNDPSDQIFLMQYFYW"
     gene            51207..53558
                     /gene="ygjK"
                     /locus_tag="ACHJWA_001460"
     CDS             51207..53558
                     /gene="ygjK"
                     /locus_tag="ACHJWA_001460"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFO4308091.1"
                     /translation="MKIKTILTPVTCALLISFSAHATNADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYNIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTSQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKTWVAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGNKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVASGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPATQ
                     YFYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHFYMLYNDFFRKQ"
     gene            54084..56102
                     /gene="fadH"
                     /locus_tag="ACHJWA_001461"
     CDS             54084..56102
                     /gene="fadH"
                     /locus_tag="ACHJWA_001461"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFO4308092.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYHNRMRFAVEVVSAVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAMQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRTPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIAGFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
BASE COUNT        13735 a        15090 c        14258 g        13025 t
ORIGIN      
        1 tctcataaaa acacatgtag caatttttta ttatttctca tttactacta cattaccgca
       61 tgattgaatg attctcaatt acatatccga tggtaaaata acaaacggat agtgcttagt
      121 gagcaacaag aaacaatttt cactcacgac aatccgtgag tttttgggat aacagactaa
      181 atggctaagt tcacaccttc attctcagga atcaaaggtc gggcgctctt ttcactgctc
      241 tttgcagcac caatgattca tgcaaccgac actgcaacga ccaaagatgg cgaaacaatc
      301 actgttacag cggatgcaaa taccgcaact gaggcgaccg atggttatca acctctgagc
      361 acttccacgg cgacattaac cgatatgccg atgctggata tcccgcaggt ggtcaatacg
      421 gttagcgatc aggttctgga aaatcagaat gcaacgacgc tggatgaagc gctttataac
      481 gtcagtaacg tggtacagac caatacatta ggcggaactc aggacgcctt tgtacgtcgt
      541 ggttttggtg ctaaccggga tggctccatc atgaccaacg gcctgcgaac tgtacttcct
      601 cgcagtttca acgccgccac agaacgtgtg gaagtgctaa aaggtccggc ctccacgctg
      661 tatggcattc tcgatcctgg tggattgatt aacgtcgtga ccaagcgccc ggaaaaaaca
      721 ttccatggtt ctgtctcagc cacgtcctcc agttttggcg gaggcactgg gcaacttgat
      781 atcacaggtc ccattgaagg cactcagctg gcataccgcc tgacggggga agtgcaggat
      841 gaagattact ggcgaaactt cggtaaagag cgcagtacat ttattgcccc gtcactcacc
      901 tggtttggtg ataatgcaac agtaaccatg ctctattccc atcgggacta taaaactccg
      961 ttcgatcgtg gaacgatttt cgaccttacg acgaaacagc ccgtaaacgt tgatcgaaaa
     1021 atacgttttg acgaaccgtt taatattaca gatggtcagt ccgatctggc gcaactcaac
     1081 gcagaatatc atctcaatag ccagtggaca gcgcgctttg attacagcta cagccaggat
     1141 aaatacagcg acaatcaggc tcgcgttacc gcgtatgatg caacgacagg aacgctgaca
     1201 cggcgtgttg atgcaactca gggatctacc cagcgtatgc attctactcg tgcggatctg
     1261 caagggaatg ttgatattgc tgggttctat aatgagattc tgggtggggt gtcatatgaa
     1321 tattatgatc ttctgcgcac agatatgatt cgctgtaaaa acgctaaaga tttcaatatc
     1381 tacaaccccg tttatggcaa taccagcaaa tgcacaacgg tttcggcgtc ggacagtgat
     1441 cagacgatca aacaggagag ctactcagct tatgcacagg atgcgctcta tctgaccgat
     1501 aactggattg ccgtcgccgg gatccgctat cagtattaca cgcagtatgc gggtaaaggc
     1561 cgtcctttta atgtcaatac tgacagccgc gatgaacaat ggacgcccaa actggggtta
     1621 gtctacaaac tgacgccatc ggtatcctta tttgctaatt attcgcaaac atttatgccg
     1681 caatcgtcaa ttgccagcta catcggagat cttccaccgg aatcatctaa tgcttacgaa
     1741 gtcggggcaa aattcgagct gttcgatggt ataaccgcag atattgcgct gtttgatatc
     1801 cataaacgta acgtgttgta taccgaaagt attggtgatg aaaccatcgc caaaacggca
     1861 ggccgcgttc gttcaagagg ggtagaagtc gaccttgcgg gagcattaac tgaaaacatt
     1921 aatatcattg ccagctacgg ctataccgat gctaaggttc tggaagatcc tgattatgca
     1981 gggaaaccat tgccgaatgt tcctcgtcat accggttcgc tattcctgac ctatgacatt
     2041 cataacatgc caggcaataa cacactgacg tttggcggtg gtggacatgg cgtaagccgt
     2101 cgttcggcaa ccaatggggc tgactattat ctgccaggct atttcgttgc cgatgccttc
     2161 gccgcataca aaatgaaatt gcagtatccg gtcacactgc aattaaacgt caaaaacctg
     2221 tttgataaaa cgtattacac ctcttccatc gccacaaata atctggggaa tcagattggc
     2281 gatccgcgtg aagtgcaatt cacggtgaaa atggaatttt gatcaaaaaa gcccggtata
     2341 cacttgtata ccgggctatg ttttattctt ctggcgggag gattatcaga cctcaatctc
     2401 cgccatgtcg cctttctctt gcaaccagtt gcggcgatct tccgagcgtt tcttcgccag
     2461 cagcatatcc atcatcgcgt cagtacgctg atcgtcttca tcatcgatag tcagctgcac
     2521 cagacggcga gtgttcggat caagcgtggt ttcgcgcaat tgcatcgggt tcatttcccc
     2581 cagaccttta aaacgctgga cgttcggctt gcctttcttg cgttttaatt gctcaagtac
     2641 gccctctttc tcttcttccg tcagcgcgta ataaacctct ttcccgagat caatacggta
     2701 gagcggtggc agtgcgacgt aaacgtgacc gtgtttcacc aacgcgcgga agtgttttac
     2761 gaacaaagcg cagagcagtg tagcaatgtg caaaccatca gagtccgcat ccgcgaggat
     2821 acagattttg ccataacgaa gctggctcag atcgtcgctg tcaggatcga taccgatcgc
     2881 taccgaaata tcatgcactt cctgcgaggc cagcacttcg tcggaagaaa cttcccaggt
     2941 gttaaggatc ttacctttca gcggcatgat cgcctgatat tcgcgatcgc gcgcctgctt
     3001 ggcagatccg cctgcggagt caccttccac gaggaacagt tcggtacggt taaggtcctg
     3061 cgcggtacaa tcagccagtt tgccaggcag cgccgggccg ctggtcagct ttttacgcac
     3121 cactttttta gccgcacgca tacgacgctg ggcgctggaa atcgccatct ctgccagcaa
     3181 ctccgccgcc tgaacgttct ggttcagcca caggatgaag gcatctttca ccacgcccga
     3241 aacgaatgcc gcacactgac gcgaagagag acgctctttg gtctgcccgg caaactgtgg
     3301 atcctgcatt tttactgaca gcacataggc gcagcgatcc cagatatctt ccgccgacaa
     3361 ctttacaccg cgcggcaaaa tgttgcggta ttcacagaac tcacgcatcg cgtccagcag
     3421 gccctgacgc aggccattaa catgggtacc gccctgcatc gttgggatca ggttgacgta
     3481 gctttcggtc agcagttcac cgccttccgg cagccacagt agcgcccagt ccaccgcttc
     3541 agtatcgcca gcgaaattac cgataaacgg tttttctggc agcgtcggta aaccgtttac
     3601 cgcttccgcc aggtaatcat tcagaccgtc ctgatagcac cagcgctgtt cggtgttgtt
     3661 gatctcatct ttaaaggtaa tctcaacgcc ggggcacagt actgctttgg ctttcagcac
     3721 atgcgtcagg cgtgaaacag aaaaacgcgg gctatcgaag aaggtttcat ccggccagaa
     3781 atggacgctg gtgccggtat tgcgtttacc gcaattgccg acaacctgta aatcctgcac
     3841 cttttcgcca ttttcaaagg cgatgttata aacctgacca tcgcggcgca cgttaacttc
     3901 tacgcgcttc gacagggcgt taaccaccga aatccccacg ccatgcaggc cgccagagaa
     3961 ctggtaattt ttgttagaga atttaccgcc tgcatgcaga cggcaaagaa tcagttcaac
     4021 cgccggtaca ccctcttccg ggtgaatatc caccggcatc ccgcgcccat cgtcaataac
     4081 ttctaacgac tggtcagcat gtaaaataac gtccacgcgt tttgcgtgac ccgccagtgc
     4141 ttcatccaca ctgttatcaa tgacttcttg ccccaaatgg ttagggcgag tggtatcggt
     4201 atacatcccc ggacggcggc gaaccggctc aagcccggtg agtacctcaa tggcatcagc
     4261 gttataagtt tgcgtcatgg tttaagttag caattcgagt tgatcgtcag agatggtgca
     4321 gaccaagaaa atcgacgatc gggttgaaat aatcttcgaa gcccgtgaat gcgtggttgc
     4381 cgccttctat gacagtctgg cggcaggaag cgtagtacgc caccgcctgg cggtaatcca
     4441 gcacttcatc tcccgtctgt tgcagcagcc agatcaaatc cggcgcttcc agcgggtcaa
     4501 tctgcatgac tttaagatcg taaatatggc gtgactctag cacatattgc tgcccggtgt
     4561 aggggttctc gttctgaccg agatagtccg tcagcagttc aaacgggcgc accgccgggt
     4621 ttaccaccac tgcgggcagc ataaaacatt gtgacaacca ggtggcgtaa tatcccccca
     4681 gtgacgaacc gacaataccc agcgaatcgc cgccatgttc caggacaatg gattccagca
     4741 gctctgccgc gtcggaagga tacggcggca actgcggaat gatcatctca acgtcagggt
     4801 gatgttccgc cagccagttt tttaacaagc tcgcttttgc agagcgcggc gagctgttga
     4861 aaccgtgtaa ataaagaagc gtagacatca gtagccttct gaagcggtat caggttggaa
     4921 acgtgtgtcc gccaggcgat gcacctcggt ggtcagcgtg ccatcagcat gtaactcgag
     4981 agtacgccag ccgggcgcga tagtatccag cgtaaagttg gaacagtgcg gcttaaactg
     5041 cacacaggtc gacggtgttg ccagcaggcg gcgaccgttc cagtcgagat ccagctcctg
     5101 atgaatatga ccgcacagca agtatttgac gtgcggaaac ttcgccagca cggtatccag
     5161 ttcgcccgcg ttacgcaggc tgtgttgatc aagccaacta caacccgcag gtagcggatg
     5221 atgatgcagc agcagcaacg tatggcgctc tggcgcatca gccagtttac gttccagcca
     5281 ctcaagctga aactcgctta gctcaccgtg cggcacgcca aacacctggc tatccagcaa
     5341 caggatttgc cattgctcac caataaacac ccgcttcgcc ggggagatac ccgcatcctg
     5401 taacgcgctg tacatcgcgg gctggaaatc gtggttgccc ggcagccaga cgcagggcgc
     5461 acgaaaactt gcgatgcctt cagcgaaatg ctgataggcc gcagaggatt gatcctgcgc
     5521 taaatcacct gtcgcgacaa tcaggtcgaa ttcgtgctgg tgtggacgaa tcgcctccag
     5581 caccgcctgg tagctttccc aggtgtttac ccctaagagg gcttcgtgct tttgtgcaaa
     5641 caggtgagta tcggtaattt gtaaaatcct gactctggcc tcaccagcca gaggaagggt
     5701 taacaggctt tccaaatggt gtccttaggt ttcacgacgc taataaaccg gaatcgccat
     5761 cgctccatgt gctaaacagt atcgcaacca gtccgctaaa aactggttaa tttgatgctt
     5821 ttcgtcgcgt tgatgcaact ttttattagg ataatcatac cgcgctttga agcgaaaaat
     5881 ctgctggctt gaacacactt cagccaccat cgcgtcatga tacagacgca ccgtcattga
     5941 cggaaggctc cagtaactga tcgcgggcgc agtctgttct attgtcacca gggtagtgta
     6001 tcgggtcgat tccacaatcg tcagccgata ttgtgcgttt gccacctgat agcttacagt
     6061 ttcgccgggt gcgtcattgc gcggtaacaa acggcgcaat tgtgaaaagt tcatctcgca
     6121 caggcgcatc atttcaggaa agtcaggtgt gtaacgcttc atttatgccc actcattttt
     6181 taacgcttga tgatgcagct gcagccattg caaagcgatg accgacgctg cgttgtcgat
     6241 tttcccctct tctacccact ggtatgcctg ttcccggctt accacatgaa cgcgaatatc
     6301 ttcgttttca tcagccagac cgtgaatacc gcttgcggtc gtggcgtcca cttcgcccac
     6361 cataattgac gaacgctcac tggtgccccc cgggcttgcc aggaaactta acaccggttt
     6421 ggtccgtttg actatcagcc ccgcctcttc aatcgcttcg cgacgggcaa catcttccac
     6481 actttcaccc tcttcaatca ttccggcaac catctccagt agccaagggg tttcgctagt
     6541 gtcgtatgcg gcaattcgaa tctgctcaat cagcacaact tcatcacgca ctgggtcaaa
     6601 gggtagcaag actgcggcgt gaccgcgctc aaaaatttcc cgccgtacct catgactcat
     6661 ttgcccgttg aatagacgat gacgaaatct ataaagatct aatgaaaaaa agccgcgata
     6721 aagtgtttct cgtgcaataa tttctacatc gtttttgcca aatgtaacgg gcaggttgtc
     6781 tggcttaagc attgttaatg tcctggcact aatagtgaat taaatgtgaa tttcagcgac
     6841 gtttgactgc cgtttgagca gtcatgtgtt aaattgaggc acattaacgc cctatggcac
     6901 gtaacgccaa ccttttgcgg tagcggcttc tgctagaatc cgcaataatt ttacagtttg
     6961 atcgcgctaa atactgcttc accacaagga atgcaaatga agaaattgct ccccattctt
     7021 atcggcctga gcctttctgg gttcagttcg ttgagccagg ccgagaacct gatgcaagtt
     7081 tatcagcaag cacgccttag taacccggaa ttgcgtaagt ctgccgccga tcgtgatgct
     7141 gcctttgaaa aaattaatga agcgcgcagt ccattactgc cacagctagg tttaggtgca
     7201 gattacacct atagcaacgg ctaccgcgac gcgaacggca tcaactctaa cgcgaccagt
     7261 gcgtccctgc agttaactca atccattttt gatatgtcga aatggcgtgc gttaacgctg
     7321 caggaaaaag cagcagggat tcaggacgtc acgtatcaga ccgatcagca aaccttgatc
     7381 ctcaacaccg cgaccgctta tttcaacgtg ttgaatgcta ttgacgttct ttcctataca
     7441 caggcgcaaa aagaagcgat ctaccgtcaa ttagatcaaa ccacccaacg ttttaacgtg
     7501 ggcctggtag cgatcaccga cgtgcagaac gcccgcgcgc agtacgatac cgtgctggcg
     7561 aacgaagtga ccgcacgtaa taaccttgat aacgcggtag agcagctgcg ccagatcacc
     7621 ggtaactact atccggaact ggcggcgctg aatgtcgaaa actttaaaac cgacaaacca
     7681 cagccggtta acgcgctgct gaaagaagcc gaaaaacgca acctgtcgct gttacaggca
     7741 cgcttgagcc aggacctggc gcgcgagcaa attcgccagg cgcaggatgg tcacttaccg
     7801 actctggatt taacggcttc tagcgggatt tctgacacct cttatagcgg ttcgaaaacc
     7861 cgtggtgccg ctggtaccca gtatgacgat agcaatatgg gccagaacaa agttggcctg
     7921 agcttctcgc tgccgattta tcagggcgga atggttaact cgcaggtgaa acaggcacag
     7981 tacaactttg ttggtgccag cgagcaactg gaaagcgcgc atcgtagcgt cgtgcagacc
     8041 gtacgttcct ctttcaacaa cattaatgct tctatcagta gtattaacgc ctacaaacaa
     8101 gccgtagttt ccgctcaaag ctcattagac gcgatggaag cgggctactc ggtcggtacg
     8161 cgtaccattg ttgatgtgtt ggatgcgacc accacgctgt acaacgccaa gcaagagctg
     8221 gcgaatgcgc gttataacta cctgattaat cagctgaata ttaagtcagc cctgggtacg
     8281 ttgaacgagc aggatctgct ggcactgaac aatgcgctga gcaaaccggt ttccactaat
     8341 ccggaaaacg ttgccccgca aacgccggaa cagaatgcta ttgctgatgg ttatgcgcct
     8401 gatagcccgg cacccgtcgt tcagcaaaca tccgcacgca ctaccaccag taacggtcat
     8461 aaccctttcc gtaactgatg acgacgacgg ggcttcggcc ccgtctgaac gtaaggcaac
     8521 gtaaagatac gggttatctg ccgcattctt cccccttctc gcttcaattt cgaccagcca
     8581 tcctctattc tgatgggtat ttaccacagg tcccggaaga caaaaatgaa acggacaaaa
     8641 tccatacgcc acgcatcgtt ccgcaaaaac tggagcgcac gccatctgac accagtcgct
     8701 ctcgcggttg ccactgtttt tatgctggct ggctgtgaaa agagtgatga aacagtgtct
     8761 ctctatcaaa atgctgacga ctgttcagct gcaaacccag gcaaaagcgc cgaatgtacc
     8821 accgcgtaca acaatgcgct gaaagaagcc gaacgtactg cgccgaaata cgccacccgt
     8881 gaagactgtg ttgctgaatt tggtgaaggt cagtgccagc aggcaccagc ccaggctggc
     8941 atggcaccag aaaaccaggc gcaggcccag caatccagcg ggagtttctg gatgccgctg
     9001 atggccggtt acatgatggg tcgtctgatg ggcggcggtg cgggatttgc acagcagccg
     9061 ctgttctcct cgaaaaaccc agccagcccg gcttacggta aatataccga cgcgacgggt
     9121 aaaaactatg gcgcagccca gccaggccgc accatgaccg taccgaagac agccatggca
     9181 ccaaaaccgg cgaccaccac taccgttacc cgtggcggtt ttggtgaatc tgttgccaaa
     9241 caaagcacta tgcagcgtag cgcaaccggt acctcttctc gttcaatggg tggctgatac
     9301 cgatggaaag agtcagtatt accgagcgcc cggactggcg tgagaaagcc cacgaatacg
     9361 gtttcaattt tcacaccatg tacggcgagc cgtactggtg tgaagatgct tactacaagt
     9421 tgaccctcgc ccaggttgaa aagctggaag aagtcaccgc cgaactgcac cagatgtgcc
     9481 tgaaagtggt ggaaaaagtg atcgccagcg atgagctgat gaccaaattc cgcattccaa
     9541 aacacacctg gagttttgtg cgccagtcat ggctgacgca ccagccatcg ctttattcgc
     9601 gtcttgatct ggcgtgggat ggcactggcg aacctaaact tctggaaaat aacgccgata
     9661 cgccaacgtc actatacgag gcggcgttct ttcagtggat ctggctggaa gatcagctta
     9721 acgccggtaa cttgccggag ggcagcgacc agtttaacag tctgcaagaa aaactgatcg
     9781 atcgcttcgt tgagctgcgt gaacagtatg gcttccagtt gctgcatctc acctgctgtc
     9841 gcgacacggt ggaagatcgc ggtaccattc agtatttgca ggactgcgcg acggaagctg
     9901 aaatcgctac cgagttcctc tacattgacg atatcgggtt aggtgaaaaa ggtcagttca
     9961 cggatttaca ggatcaggta atttccaacc tgttcaagct gtatccgtgg gaatttatgt
    10021 tgcgtgagat gttttccacc aagctggagg atgcaggcgt acgctggctg gaaccggcgt
    10081 ggaagagcat tatctccaac aaggcacttc taccgctact gtgggagatg ttcccgaatc
    10141 acccgaacct gctgcccgct tattttgcgg aagatgatca tccgcaaatg gaaaaatatg
    10201 tggttaaacc gatcttctcc cgtgaaggcg caaacgtgtc gatcattgag aacggcaaaa
    10261 ccattgaagc agcggaaggt ccgtatggcg aagaagggat gattgttcag caattccacc
    10321 cgttaccgaa attcggcgac agctatatgc tgattggtag ctggctggtg aacgatcaac
    10381 ccgccggaat tggcattcgt gaagaccgtg cattgatcac ccaggatatg tctcggtttt
    10441 atccacatat ttttgttgaa taatcaatga tgccggatgg cattcgccat ccggtaattg
    10501 ttaacctatc tgcaccgaca gcatacttaa actgcccatt tctataccat cgaccggaat
    10561 ggtaattggc tcctgcccat cccacgcacc taagacatac agcaacggca gatagtgctc
    10621 tggcgttggg ttcgataacg cgccaccttc atggtcgaga taattcacca gaggatgctg
    10681 ctctaccggc ccttgccacg tcagattcgc tttcacatac tcattaaacg acatcgccca
    10741 aggatacggt gaactatcac cgtgccactt cactgtacgc aggttatgca ccacgttgcc
    10801 gctggcgacc aacattattc cttcatcacg cagcgccgcg agttttcgcc ccatttcgaa
    10861 atgccaggcg gcaggtttgc tactgtcgat actcaactgc accatcggga tatccgcgtc
    10921 aggatacatc ttaatcagca cgccccagga accgtggtca aagccccagg cttctttatc
    10981 cagcgtcacc gggatcggcg ctaacagctc aaccagacgc tgtgccagcg caggcgaacc
    11041 cggagcagga taatgcgtat cgtacagcgc ctgcgggaag ccaccaaagt catgaatcgt
    11101 gggcggcgtc tccatcgcgg tcactcctgt tccacgggta aaccagtgag ccgaaactac
    11161 cacaatcgct tgcgggcgtg gcaatgtcat ccccaacgtc tgccagctgc gggtatacaa
    11221 attatcttcc agcacgttca tcggactacc gtgacctaaa aacaatgctg gcatacgtgt
    11281 tgaagacatg atgatatcct taactaaagg tgtcattttg atatcctcac gatacgcttg
    11341 ttcggcggag taagaacccg gataacaatg atgatgatca tcagttattt tgacgatctg
    11401 cctgaaggtg aagatttata aggagttgtc gatgtcagta cctctcattc tgaccatact
    11461 ggcgggggca gccacgttta ttggcgcgtt tctcggcgtt ctcgggcaaa aaccctcgaa
    11521 ccgcttactg gcgttttcgc taggatttgc ggcggggatc atgttgctca tctcattaat
    11581 ggaaatgctt cctgccgcac tagcagctga aggaatgtcg cctgtgttgg gttatggaat
    11641 gtttatcttc ggtctgcttg gctattttgg cctggaccgc atgttgccgc atgcgcatcc
    11701 gcaggattta atgcaaaaat cggtgcagcc gttgccaaaa tcgatcaagc gcacagccat
    11761 tctgctcact ctcggcatca gtctgcataa cttcccggaa gggattgcca cctttgtcac
    11821 ggcgagcagc aacctggagc tgggatttgg catcgcactg gccgtcgcgt tgcacaatat
    11881 tcctgaaggt ctggcggttg caggcccggt ttatgcagca acgggttcta aacgcacggc
    11941 aattctgtgg gcggggattt ctggactggc agaaattctt ggtggtgtgc tggcgtggtt
    12001 aatcctcggt agcatgattt ccccggtggt catggcagca atcatggcgg cggtcgcagg
    12061 aattatggtg gcgctctcgg ttgatgaatt aatgccgctc gccaaagaga ttgaccctaa
    12121 taataacccc agctatggcg tactatgtgg aatgtcagtg atgggattca gtttggtgct
    12181 gctacaaacg gcgggaattg gttaaaagca acatcagaag ttgtccagcg cagggacaac
    12241 ttctgctagt ttcactaaag ccttattcta ccttttcggc tgtctcttct ctcgtactgt
    12301 ttaagtattt gttccgcgct tcaagcagtg cgtctctgtc ctggcgaaat gcccgacaat
    12361 aacgtttcaa atgcaggaaa taacccacca caaccagaac aatcaataca atccagtacc
    12421 aggcgataaa catcctttta cccttaatat ttaacaatac taaacattta gcgcataaat
    12481 ttcacatatc ctttttcgga tatattcata tggtcgcata ataacggaac aattatatga
    12541 ttttgatcat aatgaaaaag aatgcctgat attaatgaaa ttttctcata gaaagagaag
    12601 cggaattaac atcttgcata gtagggaaaa ataaaaaccg aagcctgcta caacttcggt
    12661 ttcttgatta aggcagtaaa ttaagcagcg gttttcagct ggctttacgc tcatgtgcct
    12721 gacggtatgc caccaggtct tcaatagtca cgagcgccat gttgtgttta ttggcaaact
    12781 caatgcactc tggtgcacgc gccatcgtgc catcgtcatt agtcagctca cacagcacac
    12841 cagccggttt gaagcctgcc agcgtcatta gatcaatagt tgcttcagta tgaccgccac
    12901 gcgtcagtac accaccagcc tgagcgcgga gtgggaaaac gtggcccggg cgattcagat
    12961 cagaaggttt tgcgccatcg gcaatcgctg cgcgaacggt cgtaatacgg tcagcggcag
    13021 aaacaccggt agtcacacct tcagccgctt caatggtcac ggtaaaaccg gtgccatagg
    13081 cgctggtgtt attttctacc atcattggca gatcgagttg tttacggcga tcttcagtaa
    13141 tgcacaggca aacaatacca ctaccgtggc gaatggtcag cgccatctgc tcaacagtca
    13201 tggtttctgc cgggaagatc atatcacctt cgttttcacg gtcttcatca tcaagcacca
    13261 ttacaccgcg tccttcacgc agcgcagcca gtgcattttc aacacgttcg aaaggcgtac
    13321 caaaagagga aagtagcgtc tgattcatgg taaaaaaacc tcactaaaat tatggttacc
    13381 agaatcaggg cagtcttagg agtggcggca tatagccaaa ataacgtgag cgggtccatg
    13441 cccgacagaa tcgttactct ctcccatccg gactctaacc gtcggccccg gaattacacc
    13501 ggatctgctg tcctttgagt tcgcacccaa agcgctcgcg ggctttcaac tgagttgatt
    13561 taccgccggt ggggaatttc gccccgccct gagaataagc ggattcacta taacgctaat
    13621 gattagcggc agcaacgcat agcttcacat aattctggtt tatgacttac ccttatcgca
    13681 ctacaatggc actcaacacc tatcagtaca gggaaactac aatgattgac ccgaaaaaaa
    13741 ttgagcaaat cgctcgccag gttcacgaat caatgcctaa aggaatcagg gagttcgggg
    13801 aagatgtgga gaaaaaaatc cgccaaaccc tacaagcgca gctgacgcgt ctcgatctgg
    13861 taagccgcga agaattcgac gttcagacgc aagtcctgtt gcggactcgt gaaaaactgg
    13921 cgttactgga gcagcgcagc agtgaactgg aagcgcgcaa taattcggta gcagatcttc
    13981 aatctcctcc ggctatcccg cctattgata aagcagaata aagcaacaca aaaacgggcc
    14041 agacggcccg taatatcagt gttctaattc aagttctctg gtttgcatca tctgttggca
    14101 ataccaggca taacgtgcgc aaaaccaggt tctatgttct tcatccatgt taccagtaac
    14161 ggccagaata tcgaccgggc gaccttctgc gtgcattctg gcaaaactac gcgctaggaa
    14221 atcgaaatta tttaaagaat taagactatg atccataagc acatcctccg gttatcgcat
    14281 accttttaag gccatatgct ttttctgatt tcagtattcc ccaatcagtg tagtgccttt
    14341 aaacataacg tgcgtaaata tattgtcccc gatcacactt tttagtgaaa aataaaaaac
    14401 catattaaaa gactttctct tccagaacct ctctaacata tgttattatt aacaaagaca
    14461 aatgtaagca aggaaactat cacaaagtaa atattgcgtc actaaatgga cattggagtg
    14521 acatatgatt ttattcgccg actataacac cccttatctt ttcgctattt cctttgtact
    14581 gttgatcggc cttttagaaa tttttgcgct tatctgcggt cacatgctct ccggtgctct
    14641 tgatgcacat cttgaccatt atgattccat aaccacaggt catattagcc aggcacttca
    14701 ttatctcaat atcggaagat tgccagctct cgtcgttctt tgcttactgg ctggtttttt
    14761 tggtcttatt ggcatcctgt tgcagcatac ctgcatcatg gtctggcaat caccactctc
    14821 gaacctgttt gtggttcctg tcagtttact atttacgatt attgcggtgc attataccgg
    14881 taaaatagtt gcgccctgga ttccacgcga tcatagctca gctattacag aagaagaata
    14941 tattggtagc atggcgttaa ttaccggtca tcaagccact tcaggtaatc cctgcgaagg
    15001 aaaactcacc gatcattttg gccaaataca ttatttacta ctggagcctg aagaaggaaa
    15061 aattttcact aaaggagaca aagtgttaat tatttgccga ctttctgcaa cgcgatattt
    15121 ggcggaaaac aatccctagc cccaaatatt gtaatttatt aatcaaaagg aaatttaata
    15181 atggatgata ttgttaattc cgtgccctcc tggatgttta ccgcgattat tgccgtatgc
    15241 attctgttta ttattggaat tattttcgcc aggctctatc gtcgcgcttc ggcagagcaa
    15301 gcatttgtcc gtaccggctt aggggggcaa aaagtggtaa tgagcggtgg cgcaatcgtg
    15361 atgccgatct ttcatgaaat aatccccatc aatatgaata ctctgaagct ggaagtcagc
    15421 cgctcaacca ttgatagcct gattacgaaa gatcgtatgc gcgtcgatgt agtagtcgct
    15481 ttctttgtgc gggtaaaacc ttcagtagaa gggattgcca ccgctgccca gacgctgggg
    15541 caacgcaccc tgtcgcctga agacttacgt atgttggttg aagataaatt tgtcgatgcc
    15601 ctccgtgcaa cagctgcgca aatgaccatg catgagttac aggatacccg cgagaacttc
    15661 gtgcagggag tacaaaatac cgtagctgaa gatctgtcga aaaacggcct ggaactggaa
    15721 agcgtttcac ttaccaactt taaccagacc tcgaaagaac atttcaatcc taacaatgcc
    15781 tttgacgccg aaggtttaac caaactgact caggagacgg agcgccgtcg ccgcgagcgt
    15841 aacgaagttg aacaggatgt agaagttgcg gtgcgtgaga aaaaccgtga tgcactttcg
    15901 cgcaagttgg agattgaaca acaagaagcg tttatgacgc ttgagcagga gcagcaggtt
    15961 aaaacccgta ccgctgagca gaatgcgaaa attgcggctt ttgaagctga acgtcgtcgt
    16021 gaagcagagc agacgcgaat tctggctgaa cgacagattc aggaaacaga aatcgaccgc
    16081 gaacaggccg tccgctcaag aaaagttgaa gctgaacgtg aagttcgcat taaagagatc
    16141 gaacagcagc aggtcaccga aatcgctaac cagacgaaat cgatcgctat tgccgccaaa
    16201 tcggaacaac agtcccaggc agaagcgcgt gctaaccttg cactcgcaga agcagtaagc
    16261 gcccaacaaa acgtagaaac cactcgccag acagccgaag ccgatcgtgc caagcaggtg
    16321 gcgctgattg ccgcagcaca ggatgcagaa accaaagcgg ttgaactgac cgtgcgggcg
    16381 aaagcagaaa aagaagccgc agagatgcag gcggcggcga tcgttgagtt agccgaagct
    16441 acgcgtaaaa aaggcctggc ggaagcagaa gcacaacgtg cgctgaacga tgctatcaac
    16501 gtactttctg acgaacaaac cagccttaaa ttcaaactgg ccttgttgca ggcgctacct
    16561 gcggtaatag agaaatccgt ggaaccaatg aagtccattg acggtatcaa gattattcag
    16621 gtcgatggtc tgaatcgtgg tggtactgcg ggggatgcaa acacgggtaa tgtggggggc
    16681 ggaaacctgg cggaacaagc attatcagcc gcactctctt accgcacaca ggcaccactg
    16741 attgactcct tgctcaatga aattggcgtt tcaggcggct cactggctgc attgacttca
    16801 ctcttaaccc caacaactcc cgtcgccgaa aacgtagaat aaattttcag ctatgaacat
    16861 ccctggctca aaaagccggg gattttttat atctgcgttc cgctaaaagg tgcaaatgat
    16921 caggctgttg cagcgttttg cgtgattgct cggggaaggc aaaactgctt cagggaaagc
    16981 gttgcgcggg gtccggcgct catcaacaat cggggagtaa gcaaagggct gaaacgggaa
    17041 agcccctccc gaggaagggg ccttacagaa ggaaagggtt atgatgaagc tcgtcatcat
    17101 actgattgtg ttgttactcg taagtttcgc agcttattaa cagccaatca gaggggggag
    17161 aaatcctccc tcacccttgt tcctttactt taggttgaaa aaaaatcagc gtcaagaagg
    17221 tttcattaac acatatccct ggcccaaaaa gccggggatt ttttatatct gcgttccgct
    17281 aaaaggtgca aatgctcagg acgttgcagc gttttgcgtg accgctcggg gaaggcaaaa
    17341 ctgcttcagg gaaagcgttg cgcggggtcc ggcgctcatc aacaatcggg gagtcagcaa
    17401 ggggctgaaa cgggaaagcc cctcccgagg aaggggcctt atagaaggaa agggttatga
    17461 tgaagctcgt catcatactg gttgtgttgt tactgttaag tttcccgact tactaacaac
    17521 tcatcagagg ggggagaaat cctcccttac ccttgttcct ttactctagg ttgaaaagac
    17581 aacagcgtca ataggcctgc catgtacgaa gcgagatctg tgaaccgctt tccggttagc
    17641 cttttttatc ctgttggatc ttcttgataa tgttagtggt cgagcaaccg tcttcaaagt
    17701 tgagcaccaa cacttcgccg ccgttggccc agacttcttt actcccggcg atctcttccg
    17761 gtttgtagtc accgcctttc accagcaggt ctggcaggat cccggcgatc agacgctgcg
    17821 gcgtgtcttc ttcaaacgac actacccagt cgaccgcttc cagtgcgccc agcacaatca
    17881 tacgctgttc gagtgggttt accgggcggg aatccccttt cagccgtttg gtggaggcat
    17941 cgctgttgac ggcaacaatc aagcggtcac ccagcttgcg ggcatttgcc agataagaga
    18001 cgtgcccggc atgtaggata tcgaagacac cgttggtcat taccactttt tcaccacgtt
    18061 tacgcgctgc cactacagcc agcttcagtt cctcttcggt catcacgcca aagccggtat
    18121 ctgcgcgtcc acgtacagcg ttttccagct cgatcggcga aaccgtggac gtccccagtt
    18181 tgccgaccac cacaccagcc gccgcattgg caaagaagca ggcttcttcc agcgaattac
    18241 ccgctgccag cgttgccgcc aggacgccaa tcaccgtgtc gcccgcaccg gtaacgtcat
    18301 acacttcctg cgcctgagtt ggcatatgca gcggcgcttt acccggttgc agcagcgaca
    18361 taccctgttc ggaacgggtc actaacagag ccgagagttc gtaatcggca atcagtttca
    18421 tgccgcgctc aacaatctct tcttcggtct tacatttacc gacaacagct tcaaattccg
    18481 agagattcgg cgttaacagc gtagcgccgc ggtagcgctc aaaatcggta ccttttggat
    18541 caatcagcac cggaacaccc gctttacgcg ccagttggat catctgctgt acgcttgcca
    18601 gcgcaccttt ggcgtagtca gaaagcacca gcgcgccaat cgaactcagc gcctgattaa
    18661 tccgctcgtg cagcggctgc ggatcaacac cttcgaaacc ttcttcaaaa tccagacgga
    18721 tcagctgttg gttgcgggaa agtacccgta atttggtaat ggtcggatgc gtcggtacag
    18781 aaacgaagtc gcatttgacg ttgacgtcgg ccagagattt actcagcgcg cgcgctgcat
    18841 cgtcaatgcc cgtcaacccg accaggcgtg cattagcacc gagagaagcg atattcatcg
    18901 ccacgttagc cgcgccgccc ggacgttctt cgatggtatt cactttaacc acgggcaccg
    18961 gcgcttccgg cgagatacga ctggaggggc cgtaccagta acgatccagc atcacatcac
    19021 caaccaccat cactcctgca cgttcaaact ctggcagcgt tactttcatt cctgtctcct
    19081 gagagattca aaatttgcgc gcgataatac catacttcat tcttccacca gccacttctg
    19141 ccagcttgcc cgcaccagtt cacgctctgc ggtgaagcaa tcctccggca catggcccgg
    19201 caattcctgt aatgccagat gatgaagttc atcgcgaagc gtagtgtaag cacgggtcag
    19261 cgccatcgct tcctgctctt ccataatgtc gttttgcgcc agtagttcca gaatgcgcac
    19321 gttgtctgac cagcgcgtta acttcggttt ttcatgagcg tagcgcaaca ccagatattg
    19381 ggtaataaat tcgatatcgg taattccccc ttcatcagct ttgatatcaa agcgatcgcg
    19441 atgtttattg ccgagatgag cgcgcatttt ctcgcgcatt tcccgcactt ccgtttgcag
    19501 agttttacct tcacgcggca gcgtcataat ctcgcgacgc actgcgtcaa agtgcgcggt
    19561 gagctgcgga tcgccgtaca ctacacgcgc acgcaccagc gcctgatgct cccacgtcca
    19621 ggcctcgttt ttctgataat cggcaaatgc ttctgtggag gtaaccagca ttcccgccgc
    19681 cccggacggg cgcagccgcg catccacttc gtacaaaatg ccggaagagg ttcgcgtgct
    19741 gaacaggtgc ataatgcgtt gcgccagacg cagataaaac tgtcgtccgt cgatttcccg
    19801 ctcgccgtca gtcatcgcat ccattgggca atcatggagg aagatcaggt caagatcgga
    19861 actgtagcct aactcccagc cgcccagctt gccgtagccg accactgcaa aaccacgccc
    19921 ttcgcgttcg ttcaggtgat tcggcttacc gtagcgggca accatttgaa cccacgcctg
    19981 ctgaacgacg gcatctatca tggcttccgc cagccaggtt aagtgatcgc tcactttcat
    20041 caccggtagc gtaccggcga tatccgctgc ggcgatgcgt aacagctgcg cctgtttgaa
    20101 ctgacgcagc gcctcaagct gttgctcttc gtcatcttcc ggcacgcgca gcaaatactg
    20161 gcgcaactca tcgcggtagg catcggtcgc cgtcggctgg taaagggtgt ttggatcgag
    20221 caattcatcc agcaataatg gataacgcgc cagctggctg gcaatcatcg gcgacgctgc
    20281 acacagagaa atcaaatgtt taagcgccgc ggggaattca ctgagcaatt ctaaataggt
    20341 ggtgcgggta acaatccccg ccagcaaggc ggtaatgcgc aacagcgtaa cggcagcgtc
    20401 ttcacgcgca cagacatcac ttagcagatg cggcatcaga tggtcgagca cctgacgccc
    20461 gcgtggcccg attgtgcgct tatccagctc tttgcggaaa tcggcaatca gcgtcagcac
    20521 ctgtttgcga tcgtcctcgc taagatgcgc cagcactggt gtagtgtcat cttcctgcaa
    20581 cgcatcctgc cacagctcgc gccactgttc cgacagcgac tcttcctgag tttcactttc
    20641 gtcgtcgcca atcaattcat taaacacccg gcgcacattg gtcatatgtg cggtcagcgc
    20701 cccggtcaat tgcggccagt cagcaaagtc catcgcccac gccagccgcg cacggttaag
    20761 ctcatcagaa ggaagcgtct gggtttgttc gtcgttaatg ctttgcagca ggttttccag
    20821 acgccgcagg aacagatacg ccactcgcaa ttgttcagca tcgttttcag aaagcagatg
    20881 cagcgcggca atgacgctga gcgttggcag taaagagcgc gactgcagcg acggttcgcg
    20941 cccgccgcga atgagctgga acacctgaac gataaattca atttcgcgaa tgccgcctgc
    21001 gccgagtttg atattgtcgg tcaaaccacg tcgacgcact tcacgggcaa tcattccttt
    21061 catgttgcgc agcgactgaa tcacgctgaa atcgatgtaa cgacggaaaa caaacgggcg
    21121 cagcatcgca cgcaactcgt tagcatagac gccttcgcta tcgcccataa tccgcgcctt
    21181 gaccatcgcg taacgctccc agtcgcgccc ctgctcctgg taataatctt ccaacgcggc
    21241 aaagctcagc accagcgggc cactttcgcc aaacggacgc agccgcatat ccacgcgata
    21301 gacgaagcca tcctgcgttg gttgatccag cactttaatc agccgctgcc ccatgcgggt
    21361 aaaaaactgc gcgttatcca gttcccggcg tccaccctgc gtacaaccat gttccggcca
    21421 ggcaaaaatc agatcgatat cagaggagaa attcagttcc ccgccgccca gcttacccat
    21481 gcctaaaatc agcagcggtt gcgcttcgcc ctgcgcattg cacggcgttc cccactcgcg
    21541 gcagcaggcg tcatatagcc agtcacgcgc cgcaacaatc agcgtctccg ccagatagct
    21601 gagctgctgc aatatgctct cttcagtaac cagtgccagc gtttgcgccc aggcgatacg
    21661 taccatgatg cgccgacgga acagccgcag ctcgcgcatt aacccggctt cgtcactcac
    21721 attaatgagc gcctcctgca accatgcaac gtaatgctgc cattcgtcgg cctgcggcgg
    21781 ttggctttcc agttccgtca gccactctgg atgcgcactt atgctgtcct gcacaaaatc
    21841 actgaaagta agtactgact tcgcctgtgc actaagtgat tcctcggcta aaggctctgg
    21901 cagccgctca acaatggtct gccagtactg ctgtaacggt gaagagagcg gcttcataag
    21961 cgattttatc cttgcctgat taacgttttc cgctgtgcaa ccagaacggt tcctgattgt
    22021 ttgcctcatt acggaaatgt tcaatttcga tgcgttgccc ggtcgcaata gcgtgatgca
    22081 gcccctgcca gttctccagc caggcttgcg cgacgacagg atcatagtaa cccgccagca
    22141 acagtattga gtcaatatca cgcgtcaggc gtggcaactg gtcacggtag cgatcgccta
    22201 acggctggca gaaaacgctt ttcagttcag cggcatggcg ggaaagatgg atatcggcaa
    22261 agcgtttgaa ggagtcgctg attttgccct gcgctttggc atctaaaaat ggctgccatg
    22321 ctttgcttac caaccattcg gtcaacgcca gcttcgccat tgccgtttcg gtagagtaga
    22381 ccgccgtcac ggcagaaacc gcagaagcaa tggtcgcttc gcattgggtc agcaaatcac
    22441 gtaagtgagt gctcgcttta cgcggcacaa taccaccgaa cagcatcagc gtatgacgga
    22501 ccaagctaat ggctgccagc acctgttctt tcgccgcatc gttgccgcgt acccacagtt
    22561 cttcatgata ctgccattgc gctaacgcca gctcgagcgc cgcttccagc ccctgttcca
    22621 catcggcttt tgccgcaaca tgcaaaatgg tggtcggttt gatttcacgc gccggattgc
    22681 cctgcgccag atgatagcca cgcgccgctt tgctcaggct gccctggcgt aatccggttt
    22741 gcgataccag ttggttcgcc agtttcagca ccgcgcgcgt gtcgccgcta agcagctcca
    22801 gttccagctc acagataggt tcagcaaatt cccccgcttt cacctcaccc tgatcgagag
    22861 cgatttcaat tcggctgcca tcgaccgcca ccagccattt ttcgcgataa aaatcggtgc
    22921 tgaacagcgg ttgtacgcgg gaagcgagat cggcgggcaa ttcgccgttc ggccagactt
    22981 ccgtcggtaa ctgcgccagg tcgagcgtcg gttcgctcaa cgccacgtta tattccgggc
    23041 gctggtgtaa gccgcctgtc actcttcctg caactttcat ggtcatctca tagcgaccgt
    23101 tttcgccacg aatacgtaag cccatatcgt gcccacgcag ccagttatcc ggcgtttcgt
    23161 agtaaatatt cagtaactgc acggggtcat ggtgctcgcc gcccagcgta ttgagatggt
    23221 cacgcaacgc ctcaacggcg ctgtgattaa caataaactt taattcgatt tcctgagcca
    23281 tggcctttac ttatcggtta tgtcacataa gtgacgatga acggcgaact taatgcgatc
    23341 ttttttgtca gtagatagta ttttgcgcca aattgccatg caacgggcaa tttgacgggc
    23401 gtaaaagttt gaagcagtgg caagacctga cacagaggat tccgattgat gtcgaatgct
    23461 ttgcgtagcg acactgatgc cactactatc gttccacttt ccatgacaat aacgacagcc
    23521 tgatgccaaa attacgcctg atcggattaa ctttactcgc acttagcgcg actgccgtct
    23581 cacacgctga agaaacgcgc tatgtttctg acgaactgaa tacctgggtc cgtagcggtc
    23641 cgggagatca ttatcgcctc gtgggcacgg ttaacgccgg cgaggaagtg accttattac
    23701 aaactgacgc caacaccaat tatgcccagg tgaaagacag ctctggccgt accgcctgga
    23761 tcccgttgaa acaacttagc actgagccaa gcctgcgctc ccgtgtgcca gatctggaaa
    23821 atcaggtcaa aaccctgacc gataaactca ccaatatcga taacacctgg aatcagcgca
    23881 ccgcagaaat gcagcaaaaa gtggcgcaga gcgacagcgt gatcaacggg ttaaaagaag
    23941 aaaatcagaa actgaaaaac gagctgattg tcgcgcagaa aaaggtcgat gccgccagcg
    24001 tgcagctgga tgacaaacag cgcaccatca tcatgcaatg gtttatgtat ggcggcggcg
    24061 tgctggggct tggcttgctg ctcggtctgg tactgccgca cctgatccca agccgcaaac
    24121 gcaaagatcg ctggatgaac taaatcgcct tcttcgttgc actgtcttat tattaggaat
    24181 tgtgtatttg acgaagggat gatggcgtga agatttatct ggtcggtggt gctgttcggg
    24241 atgcattgtt agggctaccg gtcaaagaca gagattgggt agtggtcggc agtacgccac
    24301 aggagatgct cgacgcgggc taccagcagg taggccgcga ttttcctgtg tttctgcatc
    24361 cgcaaacgca tgaagagtat gcgctggcac gtaccgaacg gaaatccggc tccggttaca
    24421 ccggttttac ctgctatgcc gcaccggatg tcacgctgga agatgatctt aagcgtcgcg
    24481 atctgaccat taatgcgctg gcccaggacg ataacggcga gattatcgac ccgtacaacg
    24541 gtctgggcga tctgcaaaat cgtctgttgc gccatgtttc ccccgctttt ggcgaagatc
    24601 cgttacgcgt actgcgcgtg gcgcgttttg ctgcgcgtta tgcccacctc ggttttcgta
    24661 ttgccgatga aactctgacg ctgatgcgcg agatgaccca tgcgggtgaa ctggaacacc
    24721 tgacgcctga acgggtatgg aaagagacag aaaacgccct caccacccgc aatccacagg
    24781 tgttcttcca ggtactgcgc gattgcggcg cactgcgcgt tttattcccg gaaattgacg
    24841 ccctgtttgg tgttccggcc cctgccaggt ggcatccgga aatcgacacg ggtattcata
    24901 ccttaatgac gctctcaatg gcggcgatgc tgagtccgca ggtcgatgtc cgtttcacaa
    24961 ctttatgcca cgatctcggt aaaggactga cgccgccaga actctggccg cgtcatcatg
    25021 gtcatggccc ggcgggtgtt aagttagtgg aacaactatg ccagcgtctg cgcgtgccaa
    25081 atgaaattcg cgatttagcc agactggtgg ctgagtttca cgatctcatc cacaccttcc
    25141 caatgctgaa cccaaaaacc atcgtcaaat tatttgattc catcgatgcc tggcgtaaac
    25201 cgcagcgtgt cgagcaactg gcgctgacca gcgaggctga cgtgcgcggc agaaccggtt
    25261 ttgaatcagc ggactacccg caaggccgct ggttgcgcga agcctgggaa gtggcgcagt
    25321 cagtgccgac aaaagccgtc gttgaagcgg gatttaaagg tgtggagatt cgcgaggagc
    25381 tgacccgacg gcggattgcg gcggtagcca gctggaagga acaacgttgc ccaaagcctg
    25441 agtgagtatt gggcattttg ccggatgcgg cgtaaacgcc ttatccggct acatgtcaac
    25501 gacagttgta ggcctgataa gacgcgcaag cgtcgcatca agcgttgatt gccggacgcg
    25561 gcgtgaacgc cttattcggc ctacgcaatc gtggacaacg ccaggcatcc gacactattc
    25621 ctcaattaaa agaacacgac atacaccgca gccgccacaa taaagcgata aatggcgaac
    25681 gggataaacg aaatgcgctt aatcaattgc aggaaggttt taatcgctat cagcgccacc
    25741 acaaaagcgg tgataaaccc aacggcaaac atcgggatat cgccgcttgt caggaagccc
    25801 cagcttttgt agagatcgag cgccgttgcg cccatcatca tcggcaccgc cagcaggaac
    25861 gaaaactcgg aagcagcgta acggctcacc cccatcagca tcccacctga aatggtcgcc
    25921 ccggaacggg agaaacccgg ccacagcgcc agacactgga aacaaccaat cataaacgcc
    25981 tgacgatagg tcatatcatc aagacccggc gcacgcggct ctttcggctt caggcattcg
    26041 gcggcaatca gcaacaaacc gccaacgacc agcgcataca tcacatttat cgggttaaac
    26101 aatgacttaa tcgtgtcgtg gaacaacagc cccagtacca ccgccggaat catccccagc
    26161 aaaatgtgga tcagcgttaa acgacctttg ctttctcctt cgtgctgcaa cgggcggcca
    26221 aagtggatgc caatcaggcc aaacagacgc cgccagaaca tcactactac cgccagaatt
    26281 gatcctaact ggatcacaac ttcaaaggtt ttcgccgtgt cgccctcaaa ccccaacaag
    26341 tgaccgacaa taatcatatg gcccgtgctg gataccggca gaaattctgt caatccttcg
    26401 accacaccca atattgccgc tatcagcagc gagtgcatat cgctcatcaa taaaccccta
    26461 aattataaaa atgtaacgct tgtccgaact actgcagtat gaccaggtta taaccgtttg
    26521 gtttaacagc tgtaaaatta attattttct ttcagattat tgccacgctc aatgattacg
    26581 ccaacattcg ccgcccgcgc cactgcgcct ggcttgctga gtttgatacg cacccacggc
    26641 gagttgaagc gtgctaacag cagctctgcc acctcttccg ccacgcgctc caccagcgca
    26701 aaacgtgccc cctcgacgtg gctgaccacc gtttctgcaa tgtcagcgta gctgaggcaa
    26761 tccgccacat tatcgctttt tgccgcttta cggttatccc acgccatttc gatatcgaac
    26821 actaatttct gttcgatggt ctgttcccag tcgtaaacac caatagtggt gattaccgaa
    26881 agttgctcta taaatacaat atccatcacg acctgctcgc tttttggcta acccggatac
    26941 cacttccggc gaaatgtgcg tattatccac agattcatcg tttaacacga attttcaaaa
    27001 cggaacagct tatgagtgca atcgcgcctg gaatgatcct catcgcgtac ctctgcggct
    27061 ccatttccag tgccattctg gtttgccgcc tgtgtgggct gcccgatccg cgaaccagcg
    27121 gctccggtaa tcctggcgca actaacgtgt tacgtatagg tggcaaagga gcagccgtag
    27181 cagtactgat tttcgacgtt ctgaaaggaa tgttgcccgt ctggggcgcg tatgaattag
    27241 gtgtcagccc cttctggcta ggcttaattg ccatcgccgc ctgtcttgga cacatctggc
    27301 ccgttttctt cggatttaaa ggaggaaaag gcgttgctac cgcttttggt gccatcgcac
    27361 ccattggctg ggatctcacc ggagtaatgg cgggaacctg gttactgacc gtgctattga
    27421 gcggatactc gtcgctggga gcgattgtca gtgcactgat tgctccgttt tatgtctggt
    27481 ggtttaagcc acaattcacc ttcccggttt cgatgctctc ttgcctgatc ctgctgcgtc
    27541 atcatgacaa catccagcgt ctgtggcgtc gtcaggagac aaaaatctgg acgaaattca
    27601 aaagaaagcg cgaaaaggat cccgagtgat ttctggtgga tctacatgac ctgatagcct
    27661 tcatcgggtt tgcccagccg ttgctggcac catgccgcca gaaattccac gcagacacgt
    27721 aatttcatgc tgcgatagag cggctcccgg taaacagccc agatattggc gctttgtgca
    27781 tactccggca atacttgcac cagtttgcca ctctccagaa acggcaacac atcccactcg
    27841 gaacgcagca taatcccttt gccctccagc gcccattgca gcacaatctc gccgctattg
    27901 gaggaaagat gcccgctaac cttcaccgat tttttctcct gaccgttccc caactcccat
    27961 attccgtggg tcatatcgcg ctctttggtc accagacagt catgacgact taattcctgt
    28021 agggattgtg gctgcggata tttttgcaga tactcaggtg ctgcgcataa tattctttta
    28081 tttttcgtta acagatgcgc aatataataa tcaggaattt cgtcattaat acgaatatcc
    28141 agatcaatat tatcctgcac taaatcaatt tgccgatcga acaattcaaa atacacctgt
    28201 aactcaggat aattgcgcat aagttcggta atggctggcg caatatggct gcgcccaaaa
    28261 ccaaaactgc aaccaatacg aatcatcccc tccgggcgcg ttttgatttg cgtgacgtca
    28321 tcgaccagtc gctgatattg ggtgaggatt tccagcgcat gttcgtaaca acgttggcca
    28381 ctttcagtca gcgccacgcc gcgagcggag cggttcagca gcgttgttgc cagcgtattt
    28441 tcaaggattt ggatccgctt ggtgacaaat gcaggcgttt gcccaagcgt cgccgctgca
    28501 gcactaaaac tcccgctgtg gacgatttct accagtacct gcaagtcttt ggctaaaggc
    28561 cagctattca gcatcgttaa ttatccgcag ttgtgataag cgcagtgtat ttcgcaaaac
    28621 attgccattc cctgctctgg cgcaatattc attcttattt tcgcgttgat tcacgaactg
    28681 ttaattacat attcacggtg gcaaaaaata taaaaccaca tttttagtgg tagtttgtgg
    28741 cggtgaattt ttccagacaa atacaaaaac tggagttgcc atgatgagcg aaagtaataa
    28801 gcaacaggca gtgaataagt tgacggagat tgtcgctaac tttaccgcca tgatttctac
    28861 ccgaatgcct gatgacgtgg tggataaact aaaacagcta aaggatgccg aaacgtcgtc
    28921 gatggggaaa attatctacc atacgatgtt cgacaacatg caaaaagcga ttgacctgaa
    28981 tcgtcctgcc tgtcaggaca ccggggagat tatgttcttc gttaaagtcg gttcccgctt
    29041 cccactgctt ggcgagctgc aaagcatact caaacaagcc gtggaagagg cgaccatcaa
    29101 agcgccgctg cgtcacaatg cggtagaaat ttttgacgaa gtaaacaccg gcaaaaatac
    29161 cggtagcggc gtaccgtggg tcacctggga tatcgtcccc gacggtgacg atgcggaaat
    29221 cgaagtttac atggcaggcg gcggctgcac gctaccaggc cgctcgaaag tgttaatgcc
    29281 gtcagaaggc tacgaaggcg tggtgaaatt cgtcttcgaa aatatctcca ccctcgcagt
    29341 aaacgcctgt ccaccggtac tggtgggcgt gggcatcgcc acctcggtgg aaaccgccgc
    29401 cgtactctcg cgtaaagcca ttttgcgccc gattggcagc cgccacccca atccaaaagc
    29461 ggcagagctg gagctacgcc tggaagaagg actcaaccgt ctggggattg gtccacaagg
    29521 gctgactggc aacagttcag tgatgggcgt gcatatcgaa tctgccgccc gccatccgtc
    29581 aaccatcggc gttgctgttt ctaccggttg ctgggcgcat cgtcgcggca cactgctggt
    29641 tcatgccgat ctcacctttg aaaatctgtc tcacacccgg agcgcgttat gaaaaagatc
    29701 ctgacaaccc cgatcaaagc tgaagatctg caagatattc acgtcggcga tgtgatctat
    29761 ctgaccggta cgctggtgac ctgccgtgac gtttgtcacc gccgattgat cgaactgaaa
    29821 cgtccgctcc cttacgatct caacggcaaa gcgattttcc acgctggccc catcgtgcgc
    29881 aaaaacggcg acaaatggga gatggtctcc gtcggtccga caaccagtat gcgtatggaa
    29941 agttttgaac gtgaatttat cgagcagacc ggcgtgaaac tggtggtcgg caaaggcgga
    30001 atggggccgc tgaccgaaga aggctgtcag aaattcaagg cgctacatgt aattttcccg
    30061 gcaggctgcg cggtgctggc ggcaacccag gtggaagaga ttgaagaagt gcactggaca
    30121 gaactcggga tgccggagtc actgtgggtc tgccgggtca aagagttcgg cccgctgatt
    30181 gtctctattg atacccacgg caacaacctg atagccgaaa acaaaaagct gttcgccgaa
    30241 cgccgcgatc ccatcgtgga agagatctgc gagcacgtcc attacatcaa ataaccctcc
    30301 cggagaggct cgccccctct cctttttcgc aggcataaca cgatgaaacc ttccactgaa
    30361 tggtggcgat accttgcgcc gctggcggtc atcgccatta ttgctctaat tccggttccc
    30421 gcagggctgg agagtcatac ctggctctac tttgccgttt ttactggcgt aatcgttgga
    30481 ctgatcctcg aacccgtgcc gggtgccgtg gtggcgatgg tgggtatctc catcatcgcc
    30541 atactctctc cctggctgct gttcagcccg gagcagctcg ctcagccagg ctttaaattc
    30601 actgcaaaat ccctctcgtg ggccgtttcc ggtttttcta attcggttat ctggctgatt
    30661 ttcgccgcct ttatgtttgg cacaggctat gaaaaaaccg ggctggggcg acgtatcgcg
    30721 ctgattctgg tgaaaaagat ggggcatcgc acgctgtttc ttggctatgc ggtgatgttc
    30781 tccgagctga tcctggcacc tgtcacaccg tccaactcgg cgcgtggtgc ggggattatc
    30841 tatcccatca tccgtaacct gccgccgctc tatcaatcac aaccaaacga cagcagttcg
    30901 cgcagtattg gctcgtacat catgtggatg gggattgttg ccgactgcgt gaccagcgcc
    30961 attttcctga cggcgatggc tcctaacttg ctgttaattg gactgatgaa aagcgcatct
    31021 cacgccacgc tgagttgggg cgactggttc ctcgggatgt tgccgctcag tattttactg
    31081 gtcctgctgg ttccctggct ggcttacgtg ctgtacccgc ctgtactgaa gtcaggcgat
    31141 caggtgccgc gctgggcaga gacggaactg caggcaatgg gcccgctctg ttcgcgtgaa
    31201 aaacggatgc tggggctgat ggtaggcgcg ctggtgctgt ggattttcgg cggtgattat
    31261 atcgatgccg cgatggtcgg ttacagcgtg gtggcgctga tgctgcttct gcgcattatc
    31321 agttgggacg atattgtcag taataaagcg gcgtggaacg ttttcttctg gctggcctcg
    31381 cttatcaccc tcgcgaccgg actcaacaac accggtttta ttagctggtt tggcaaactg
    31441 ttagcaggca gcttaagcgg ttattcgcca acgatggtga tggtggcgtt gattgtggtg
    31501 ttttatctac tgcgctactt ttttgccagc gccacggcgt atacctccgc tctcgcaccg
    31561 atgatgattg ccgctgcgct ggcgatgccg gaaatcccgc tgccggtgtt ctgcctgatg
    31621 gttggtgcgg caattggtct ggggagcatt cttacaccat acgccaccgg ccccagtccg
    31681 atttactacg gcagtggtta tctgccaacg gcggattact ggcgactggg ggcgattttt
    31741 gggctgatat tcctcgtatt gctggtgatt accggcttac tgtggatgcc cgtggtgttg
    31801 ctttaagtaa ggacggtgcc ggaagtaact ctccggcacc cgatagtttt acgcagccgg
    31861 taactccgcc agcggccagc gcggacgcac gctaacgccg agatccgccg tcgcgcctgc
    31921 tttaaaccgc accattccgg cataggcgat catcgcgccg ttatcagtac aaaattccgg
    31981 acgcgcgtag aacacttcgc cgcggcgttt tttcatcatt tcagccagct tcgcccgtaa
    32041 cgtgcggtta gcactcacgc cgcccgccat gaccagtcgc ttaaagcccg tctgatccag
    32101 cgcacgctta cacttaatca tcaacgtatc gaccaccgca tcttcaaagg cgcgggcgat
    32161 gtcagcacgc gtctggtcgt cggtgccgtt gtcacgaatg gtatttgccg cgaaggtttt
    32221 caggccggag aagctgaaat ccagccccgg acggtcggtc atcggacgcg ggaaaacaaa
    32281 gcgcccggca gtaccctgcg ccgccatttt cgacagtaac ggtccgccag gataatccag
    32341 ccccagcagc ttcgcggttt tatcaaacgc ttcaccggcg gcatcatcga tagactcgcc
    32401 gagcagctcg tactgaccaa tgccagtcac gctgattaac tgcgtatgac cgccggaaac
    32461 cagcagcgca acaaacggaa attccggcgg gttatcttcc agcatcggcg ctaacagatg
    32521 cccttccata tggtgtacag ggatcgccgg aacgttccag gcaaacgcca gagaacgccc
    32581 cacggtcgcg ccaaccagca gtgcgccgac taatccaggg cctgcggtat aggccacagc
    32641 atcaatgtct tttgccgtta aaccagactc ctttagcgcc gcctggatca acggtacggt
    32701 tttacgcaca tgatcgcggg aggccagttc aggcacaacg cccccgtagt cagcgtgcaa
    32761 tttcacctga ctatacaatt ggttggctaa caaacctttt tcatcgtcgt aaatggcgat
    32821 gccggtttca tcgcaggaag tttcaatacc cagtacacgc atgacttgtt ttacctcgct
    32881 ttattaccgc gcagtgtagg accaatgcgg gttgatgtaa aactttgttc gcccctggag
    32941 aaagcctcgt gtatactcct cacccttata aaagtccctt tcaaaaaagg ccgcggtgct
    33001 ttacaaagca gcagcaattg cagtaaaatt ccgcaccatt ttgaaataag ctggcgttga
    33061 tgccagcggc aaaccgaatt aatcaaaggt gagaggcaca tgccggtaat taaagtacgt
    33121 gaaaacgagc cgttcgacgt agctctgcgt cgcttcaagc gttcctgcga aaaagcaggt
    33181 gttctggcgg aagttcgtcg tcgtgagttc tatgaaaaac cgactaccga acgtaagcgc
    33241 gctaaagctt ctgcagtgaa acgtcacgcg aagaaactgg ctcgcgaaaa cgcacgccgc
    33301 actcgtctgt actaattccc cgagagcgtt gctctccgat cagaccgagt tgtagttgta
    33361 aggccgtgct tccgaaagga atgcgcggct tattttcgtt tatgaattgc taaaaatcgg
    33421 ggcctatggc tggacgaatc ccacgcgtat tcattaatga tctgctggca cgcactgaca
    33481 tcgtcgatct gatcgatgcc cgtgtgaagc tgaaaaagca gggcaagaat ttccacgcgt
    33541 gttgtccatt ccacaacgag aaaaccccgt ccttcaccgt taacggtgag aaacagtttt
    33601 accactgctt tggatgtggc gcgcacggca acgcgatcga cttcctgatg aactacgaca
    33661 agcttgagtt cgtcgaaacg gtcgaagagc tggcggcaat gcacaatctt gaagtgccat
    33721 ttgaagcagg tagcggcccc agccagatcg agcgccatca gcggcaaacg ctttatcagt
    33781 tgatggacgg tctgaatacg ttttaccaac aatctttaca acaacctgtt gccacgtctg
    33841 cgcgccagta tctggaaaaa cgcggattaa gccacgaggt tatcgctcgc tttgcgattg
    33901 gttttgcgcc tcccggctgg gacaacgtcc tgaagcggtt tggcggcaat ccagaaaatc
    33961 gccagtcatt gattgatgcg gggatgttgg tcactaacga tcagggacgc agttacgatc
    34021 gtttccgcga gcgggtgatg ttccccattc gcgataaacg cggtcgggtg attggttttg
    34081 gcgggcgtgt gctgggcaac gataccccca aatacctgaa ctcgccggaa acagacattt
    34141 tccataaagg ccgccagctt tacggtcttt atgaagcgca gcaggataac gctgaaccca
    34201 atcgtctgct tgtggtcgaa ggctatatgg acgtggtggc gctggcgcaa tacggcatta
    34261 attacgccgt tgcgtcgtta ggtacgtcaa ccaccgccga tcacatacaa ctgttgttcc
    34321 gcgcgaccaa caatgtcatt tgctgttatg acggcgaccg tgcaggccgt gatgccgcat
    34381 ggcgagcgct ggaaacggcg ctgccttaca tgacagacgg ccgtcagcta cgctttatgt
    34441 ttttgcctga tggcgaagac cctgacacgc tggtacgaaa agaaggtaaa gaagcgtttg
    34501 aagcgcggat ggagcaggcg atgccgcttt ccgcatttct gtttaacagc ctgatgccgc
    34561 aagttgatct gagtacccct gacgggcgcg cacgtttgag tacgctggca ctgccattga
    34621 tatcgcaagt gccgggcgaa acgctgcgaa tatatcttcg tcaggaatta ggcaacaaat
    34681 taggcatact tgatgacagc cagcttgaac gattaatgcc aaaagcggca gagagcggcg
    34741 tttctcgccc tgttccgcag ctaaaacgca cgaccatgcg tatacttata gggttgctgg
    34801 tgcaaaatcc agaattagcg acgttggtcc cgccgcttga gaatctggat gaaaataagc
    34861 tccctggact tggcttattc agagaactgg tcaacacttg tctctcccag ccaggtctga
    34921 ccaccgggca acttttagag cactatcgtg gtacaaataa tgctgccacc cttgaaaaac
    34981 tgtcgatgtg ggacgatata gcagataaga atattgctga gcaaaccttc accgactcac
    35041 tcaaccatat gtttgattcg ctgcttgaac tgcgccagga agagttaatc gctcgtgagc
    35101 gcacgcatgg tttaagcaac gaagaacgcc tggagctctg gacattaaac caggagctgg
    35161 cgaaaaagtg atttaacggc ttaagtgccg aagagcgatc gggaagcccc cgacagccgc
    35221 actgagaggc agcggcaaat atataagtac gccctcgtaa ttatcgttgg cggtaaacaa
    35281 ccgttggatt tcagcgttaa cggctgaagg acatcgggtc aatcgcccaa caccaacctc
    35341 atgaaataag tgtggatacc gtcttatgga gcaaaacccg cagtcacagc tgaaacttct
    35401 tgtcacccgt ggtaaggagc aaggctatct gacctatgcc gaggtcaatg accatctgcc
    35461 ggaagatatc gtcgattcag atcagatcga agacatcatc caaatgatca acgacatggg
    35521 cattcaggtg atggaagaag caccggatgc cgatgatctg atgctggctg aaaacaccgc
    35581 ggacgaagat gctgccgaag ccgccgcgca ggtgctttcc agcgtggaat ctgaaatcgg
    35641 gcgcacgact gacccggtac gcatgtacat gcgtgaaatg ggcaccgttg aactgttgac
    35701 ccgcgaaggc gaaattgaca tcgctaagcg tattgaagac gggatcaacc aggttcaatg
    35761 ctccgttgct gaatatccgg aagcgatcac ctatctgctg gaacagtacg atcgtgttga
    35821 agcagaagaa gcgcgtctgt ccgatctgat caccggcttt gttgacccga acgcagaaga
    35881 agatctggca cctaccgcca ctcacgtcgg ttctgagctt tcccaggaag atctggacga
    35941 tgacgaagat gaagacgaag aagatggcga tgacgacagc gccgatgatg acaacagcat
    36001 cgacccggaa ctggctcgcg aaaaatttgc ggaactgcgc gctcagtacg ttgtaacgcg
    36061 tgacaccatc aaagcgaaag gtcgcagtca cgctgccgct caggaagaga tcctgaaact
    36121 gtctgaagta ttcaaacagt tccgcctggt gccgaagcag tttgactacc tggtcaacag
    36181 tatgcgcgtc atgatggacc gcgttcgtac gcaagaacgt ctgatcatga agctctgcgt
    36241 tgagcagtgc aaaatgccga agaaaaactt cattactctg tttaccggca acgaaaccag
    36301 cgatacctgg ttcaacgcgg caattgcgat gaacaagccg tggtcggaaa aactgcacga
    36361 tgtctctgaa gaagtgcatc gcgccctgca gaaactgcag cagattgaag aagaaaccgg
    36421 cctgaccatc gagcaggtta aagatatcaa ccgtcgtatg tccatcggtg aagcgaaagc
    36481 ccgccgtgcg aagaaagaga tggttgaagc gaacttacgt ctggttattt ctatcgccaa
    36541 gaaatacacc aaccgtggct tgcagttcct tgacctgatt caggaaggca acatcggtct
    36601 gatgaaagcg gttgataaat tcgaataccg ccgtggttac aagttctcca cctacgcaac
    36661 ctggtggatc cgtcaggcga tcacccgctc tatcgcggat caggcgcgca ccatccgtat
    36721 tccggtgcat atgattgaga ctatcaacaa actcaaccgt atttctcgcc agatgctgca
    36781 agagatgggc cgtgaaccga cgccggaaga actggctgaa cgtatgctga tgccggaaga
    36841 caagatccgc aaagtgctga agatcgccaa agagccaatc tccatggaaa cgccgatcgg
    36901 tgatgatgaa gattcgcatc tgggggattt catcgaggat accaccctcg agctgccgct
    36961 ggattctgcg accaccgaaa gcctgcgtgc ggcaacacac gacgtgctgg ctggcctgac
    37021 cgcgcgtgaa gcgaaagttc tgcgtatgcg tttcggtatc gatatgaaca ccgatcacac
    37081 gctggaagaa gtgggtaaac agttcgacgt tacccgtgaa cgtatccgtc agatcgaagc
    37141 gaaggcgctg cgcaaactgc gtcacccgag ccgttctgaa gtgctgcgta gcttcctgga
    37201 cgattaatcg gtaggccgga tcaggcgtta cgccgcaccc ggcacctgtc cctttgcaca
    37261 aacgccacct tttcggtggc gttttttatc gcccacgcac caccagcgcc tggtccagct
    37321 cgcgatacgc ttcaaccagt ttctccagtg aaacgcgact taaaccgctg ggatttggca
    37381 gcacccaaat ctgcgtcgaa ccaatgctga gcgtttgttt cccccactgt gcaccacgct
    37441 ggctgaatcc ctgttcatat gcttgtttgc ccagaatcgc caacgcttgc ggctgataat
    37501 cttcaatttt ttcaatcagc ttacgcccac ctgcgtgtag ctcttgcttt gaaatttcat
    37561 tggcttgcac cgttggcctg tctaccagtt tggtgacgcc acaacgataa tccagcagat
    37621 gctgtgcctc ctgcggcttc aactgatggt cggtaaaccc ggcctgatat atcaccttcc
    37681 agaagcgatt tgccggatga gcaaagggaa aaccagtccc ggcggatgaa agcccagggt
    37741 tgataccgca aaacacgacc cgtaaccctg gagccaaaat atcctcaacc atatttactc
    37801 ctttctaaca tcaccaggaa aagtataacg gattgaaaat acattgttta taaaaacagc
    37861 aggcgcgcgg taatggctgg attgcgacac ggagttactt tataatccgc taccatggcc
    37921 ccttagctca gtggttagag caggcgactc ataatcgctt ggtcgctggt tcaagtccag
    37981 caggggccac cagatatagc aaaggctgac gagaaatcgt cagccttttt ctttttatat
    38041 atcagttact ttgcgtgcca gtaggccgct gcacgtaccc gctgtgggtc atactgttcc
    38101 gcttcaaagc ggcggcttaa attcttaacg actttacctt cgccggttat ccagatgaag
    38161 taatcatcgg cagggatttg catctgcgcc agacgcgcat ctaccgcctg ctcatcatgt
    38221 gccagccatt cgatattaaa accatcaagg tgcgcgagat aatcctgaca ggcgttatcc
    38281 cgcacgctaa ccagcgcact aacttgcggt ttaacggcaa gtttgctcaa cgtttccagg
    38341 cggcggcgca atgcgggcat tccggattca tcgcagacat acagctgata cgcgtaatct
    38401 tccggcacca ccagcgaacc gcgcggacct gccaccgtaa gtttatcgcc cggttgcgcc
    38461 tgcatcgccc agccgctggc gaccccaccg tcgtgaataa agaaatcgat cgccagttca
    38521 tggcgtagtt cgtcatacag cggcgtatag tcacgcgacg gtgggcgtgg tccttccggc
    38581 cagacgatgc cctcttccgt taccgttggc ggcacaaagt gagcgtcagg ttgaggaaag
    38641 aagagtttgc tgtgatcgtc aaagccgcgc gatgtaaaac cgtccagcgc ctcgccgcca
    38701 aggacaatgc gctgaaaacc ggcgctgata cgctcaacgc gtagcacagt caattcacgg
    38761 aagcgcagat cattgcgaac gcgctgcggg tagcgggggg agttattcat tgttatcgcc
    38821 ttcgtgatgg taatcagata tatctaaata attttcgcaa atgataatga ttgttaatca
    38881 tgataaatgc aagcgatttg tagaactgat atgtctatag tctgacaaga cgaaccgcct
    38941 cttctcaggc atcattactc aacgccggat gcggcgtgaa cgccttatcc ggcctacgtg
    39001 tgagatgagt ccacatttaa aaatcatttt tattcttgca agagtgctca catcagatat
    39061 aaattagata tatctaatta agcaaaagga ggctgatatg agccatcatc acgaagggtg
    39121 ttgtaaacat gaaggccagc cacgccatga gggctgctgc aaaggtgaga agtcagaaca
    39181 cgagcactgc ggacacggtc accagcatga acacggtcaa tgttgcggtg gtcgccacgg
    39241 tcgcggcggc ggtcgtcggc aacgtttctt tggtcacggt gaattgcgtc tggtgattct
    39301 ggatattctc tcgcgcgatg acagccacgg ttacgaattg attaaagcga ttgagaatct
    39361 aacccaaggg aattacaccc caagcccggg cgtcatctac ccgacgctgg attttctgca
    39421 ggagcagtcg ctgattacca tccgcgaaga ggaaggtggt aagaagcaga ttgcgctgac
    39481 cgaacaaggc gcgcagtggc tggaagaaaa ccgcgaacag gtggagatga ttgaagaacg
    39541 catcaaagcg cgttgcgttg gcgctgcgct gcgccagaac ccgcaaatga agcgggcgct
    39601 agataatttt aaagcggtgc tgaatttacg cgtcaaccag agtgatatca ctgatgcaca
    39661 aataaaaaag atcattgcgg tgatcgaccg cgccgctttt gatattacgc aactggatta
    39721 atcgccgcat ccgccagtgg cgcggtgcaa ttgccggatg cgacgcttga cgcaccttat
    39781 ccggcctaca cccgctacac accccgtagg cctgataaga tgcgccagca tcgcatcagg
    39841 catcgtgtat caaataccgg atccggcata ccgattaatg cagcaccgtc accgcgtctt
    39901 ccagtcggct ggcgcggtgt ttcaccatcg ccgacacctg cgcactctct tccaccagct
    39961 cggcattttt ctgggtgatg aggttaagct catccactgc acgggtcagg ctggaaagac
    40021 catcggcctg ttccagcgtt gaatggctaa tctgggcaat caactgggtg acgtttttca
    40081 cctgtgccac aatatcttcc atcgtacgtc cggcggcgtg tacctgctgc gaaccggatt
    40141 gcaccttatc agcactggca tcaatcagct tgcgaatatc gttggcagca ttggcgctgc
    40201 ggctggctaa atgacgcact tcccccgcca ccaccgcaaa acctttgccc tgttcgccgg
    40261 cacgcgccgc ttccaccgcc gcattcaggg ccagaatatt ggtctgaaac gcaatatcgt
    40321 taatcagcga agtaatggtg ccaatgcgct gggtactgtc ggcgatatcg tccattgtct
    40381 tgatcaccgt ggtcatcgcc tccccgccct gcactgccgc attactggcg gtgattgaca
    40441 gtttatcggc agccgacgcc gtggcagagt tctgtttcac cgacgccgcc atttggttca
    40501 tggtcgccac cgtttgctga acgttatcaa ctgtctgctg ggtatgttcg ttcagttcat
    40561 cggtgccttt cgccagcgtc tcactgccgt ttctgacact ggacacctgg cttgagacat
    40621 cgttaattaa ccaacggcac atcaggccaa gctgccctac cgcgcgtaat gtcagcccca
    40681 gctcatcgct gcgattcaga tgctcaacac tattacgctc tccggtcgcc accttcagtg
    40741 cctgacgggc gacattttct atcgggcgga caatctgcca ttcaaaacag gcgcttgcca
    40801 acaataccac taacgcacag aggatatacg tcaccaccgg ggcagcaaca aaccaaagca
    40861 tggccgccag caagataaac atcagggtca tcactccacg cgcccgccag cgaagcggta
    40921 atgaaggcag tttacccagc cagcctttac gcaccaccag gcctttatga atacgcttac
    40981 cggtacgtcc ggcgttcagc gctttgtaca gcggctccac cgccgcgatc tcttcatccg
    41041 tcgcccgggt acgaatcgac atatagccac tgatttttcc ctcgcgcacc atcggtaccg
    41101 cattggcccg cacccaataa tggtcaccat ttttgcggcg atttttcacg atgccgctcc
    41161 agggctcccc ttttttcagg gtgaaccaca tatccgcaaa cgccgctttt ggcatatccg
    41221 ggtgacgcac catgttgtgc ggctgccctt gtaactcttg caaggtaaag ccgctcacct
    41281 gcacaaaagt gtcattagca tgagtgatat agctttgcag atcggtagtg gacatcagag
    41341 tggtatcgtc cgccagcggg gtattttgct gggtgacata cggatgagaa gacatgttcg
    41401 cgtcctgtgc aggttatatg gttgttaact tcttgtcaga gtttatgtcg gccccgctgc
    41461 ggttatcttt aaccgattaa tttgatttag atcgcaattt gcgattaaag cacaaatcta
    41521 attcgttgat ttaaaatact ttcactctgt tactatacga aaacgttaat tatcttgccc
    41581 aaaaatcagg cagttaatgc cctgaaagcg tgcatttgcg cagcaattat caaatccata
    41641 cccgacaaaa accgtgcaaa ataacaacag atgttaacaa atagcattaa atattgcgca
    41701 aatgataacc acatttgtgt ttatcccgat tttcgcgatc gcagccggag tggcgcaatc
    41761 cctgcaatac ttaaatcggt atcatgtgat acgcgagcct ccggagcata ttttgaacag
    41821 gttaccttcg agcgcatcgg ctttagcgtg cagcgcccac gccctgaatc tcattgaaaa
    41881 gcgaacgctg gatcatgagg agatgaaagc acttaaccga gaggtgattg aatacttcaa
    41941 agagcatgtc aatccggggt ttttagagta tcgcaaatct gttaccgccg gcggggatta
    42001 cggagccgta gagtggcaag cgggaggttt aaatacgctt gtcgacaccc agggacagga
    42061 gtttatcgac tgcctgggag gttttggaat tttcaacgtg gggcaccgta atccagttgt
    42121 ggtttccgcc gtacagaatc aacttgcgaa acaaccactg cacagccagg agctactcga
    42181 tccgttacgg gctatgctgg cgaaaaccct tgctgcgcta acgccaggta aactgaaata
    42241 cagcttcttc tgtaatagcg gcaccgagtc cgttgaagcg gcgctgaagc tggcgaaggc
    42301 ttatcagtcg ccgcgcggca aatttacttt tattgccacc agcggcgcgt tccacggtaa
    42361 atcacttggc gcgctgtcgg ccacggcgaa atcgaccttc cgcaaaccgt ttatgccact
    42421 gctgccaggg ttccgtcatg tgccgtttgg caatatcgaa gccatgcgca cggctcttaa
    42481 tgagtgcaaa aaaaccggtg atgatgtggc cgcggtgatc ctcgaaccga ttcagggcga
    42541 aggtggcgta attctgccgc cgccgggcta tctcaccgcc gtgcgtaagc tgtgcgatga
    42601 gttcggcgca ctgatgatcc tcgatgaagt acaaacgggc atggggcgca cgggcaagat
    42661 gttcgcctgc gaacatgaga acgtacagcc ggacatcctg tgcctggcaa aagccctcgg
    42721 cggcggtgtg atgccgattg gcgcaaccat cgccactgaa gaggtgttct cagttctgtt
    42781 cgacaacccg ttcctgcata ccaccacctt tggcggcaac ccgctggcct gtgcggcggc
    42841 gctggcgacc atcaatgtgt tgctggagca gaacttaccg gctcaggctg agcaaaaagg
    42901 cgatatgttg ctggacggtt tccgtcaact ggcgcgggaa tatcccgatc tggtacagga
    42961 agcgcgtggt aaagggatgt taatggcgat tgagtttgtt gataacgaaa tcggctataa
    43021 ctttgccagc gagatgttcc gccagcgcgt actggtggcc ggaacgctca ataacgccaa
    43081 aacgatccgc attgaaccgc cactgacact gaccattgaa cagtgtgaac tcgtgatcaa
    43141 agcggcgcgt aaggcgctgg cggccatgcg agtaagtgtc gaagaagcgt agtatcaaat
    43201 cggatggcaa tacgacgtcg ccatccgatc tttttatacg atacgtacgc ccgcaggcat
    43261 catccgctcc ggcgttaaca gcacgctttc actgccgtca tcggtttccg cacacaacag
    43321 catgcattcc gacgtttcac cacgcatttt cgctttttgc agattgcata agaccaccac
    43381 cgtttttccc atcaactctt cttcgctgta gtacggcacc aggctggtca cggtttgcag
    43441 tgttttttcc cccacatcaa cctgtacgat gtacagcttg tcggcgtttt catggcgttt
    43501 cacttccaca atctttccga cgcgcatttc cagacgtgca aaatcagcgt aagccacggt
    43561 ttccattgct tcctcccttc gagtaaaatt ttactaaact atagcaaaag tttttctcaa
    43621 tcctgtaggc taaaaatgga gaatgcaggc gtgatcacat tcgtgagccg ctgtgttacc
    43681 gttacagcgt caaagaaacg cgctttattt actgaaaaca ggtgacccga taagcacttc
    43741 ctctacaatg ggggcgcaca tcagggaaag taaaaaaggt aaacatggca acactaaaag
    43801 acatcgcaat cgaagctggc gtatccctgg cgacagtatc cagggtctta aatgacgatc
    43861 cgacattgaa tgtgaaagaa gagacgaaac atcgcattct cgagatcgcc gaaaagctgg
    43921 agtacaagac cagtagcgcc cgtaaactac agacaggcgc agtcaaccaa caccatattc
    43981 tggctatcta cagctaccag caggagctgg agatcaacga tccttactat ctggcgatcc
    44041 gccacggcat tgaaactcag tgcgaaaagc tggcgatcga actgaccaac tgttatgaac
    44101 acagcggatt accagacatt aaaaacgtca ccggtatttt aattgtcggc aaacccacgc
    44161 ccgccctgcg cgccgctgcc agcgcgttga ccgacaatat ctgttttatc gactttcacg
    44221 aacccggcag cggttacgat gcggtggata tcgatctggc acgcatcagt aaagaaatca
    44281 tcgacttcta tatcaaccag ggcgttaatc gtattggttt tattggcggt gaagatgagc
    44341 ctggcaaggc ggatattcgt gaggtcgcct ttgcggaata tggccgactg aaacaagtgg
    44401 tacgcgaaga ggatatctgg cgcggcggtt tttccagttc gtcgggttat gaactggcaa
    44461 aacaaatgct ggcgcgggaa gactatccga aggcactgtt tgttgcttcc gattccattg
    44521 ctatcggcgt actgcgggcg attcatgaac gtggtctgaa catcccacag gatatttcgc
    44581 ttatcagcgt taacgatatc cccaccgcgc gatttacctt tccgccgctc tccaccgtgc
    44641 gcatccattc cgaaatgatg ggaagtcagg gcgtaaacct ggtgtatgaa aaagcccgcg
    44701 atggtcgcgc gctgccgctg ttagtcttcg ttcccagcaa attaaaactg cgcggcacga
    44761 cccgttaaat ccccttacac actgtccggc aatcgttttt gccgggcagt gctgccgttt
    44821 attttcgtga tccagttaaa gtaaatgcat ttacccgcta ctttttagta aaaattttac
    44881 taaactcccc agcaattaca caaactacca tcaccatgaa tggttccgat ttctctctac
    44941 cgggaggccc tatgaatcgc tgggaaaaca ttcagctcac ccacgaaaac cgacttgcgc
    45001 cgcgtgcgta ctttttttca tatgattctg ttgcgcaagc gcgtaccttt gcccgcgaaa
    45061 cgagcagcct gtttctgccc ttaagcggtc agtggaattt ccactttttt gaccatccgc
    45121 tgcaagtgcc agaagccttc acctctgagt taatggctga ctgggggcat attaccgtcc
    45181 ccgccatgtg gcaaatggaa ggtcacggca aactgcaata taccgacgaa ggttttccgt
    45241 tccccatcga tgtgccgttt gttcccagcg ataacccaac cggtgcctat caacgtattt
    45301 tcaccctcag tgacggctgg cagggtaaac agacgctgat taaatttgac ggcgtcgaaa
    45361 cctattttga agtctacgtt aacggtcagt atgtgggttt cagcaagggc agtcgcctga
    45421 ccgcagagtt tgacatcagc gcgatggtta aaaccggcga caacctgttg tgtgtgcgcg
    45481 tgatgcagtg ggcggactct acctacgtgg aagaccagga tatgtggtgg tcagcgggga
    45541 tcttccgcga tgtttatctg gtcggaaaac acctaacgca tattaacgat ttcactgtgc
    45601 gtaccgactt tgacgaagcc tattgcgatg ccacgctttc ctgcgaagtg gtgctggaaa
    45661 atctcgccgc ctcccctgtc gtcacgacgc tggaatatac cctgtttgat ggcgaacgcg
    45721 tggtgcacag cagcgccatt gatcatttgg caattgaaaa actgaccagc gccagctttg
    45781 cttttactgt cgaacagccg cagcaatggt cagcagaatc cccttatctt taccatctgg
    45841 tcatgacgct gaaagacgcc aacggcaacg ttctggaagt ggtgccacaa cgcgttggct
    45901 tccgtgatat caaagtgcgc gacggtctgt tctggatcaa taaccgttat gtgatgctgc
    45961 acggcgtcaa ccgtcacgac aacgatcatc gcaaaggccg cgccgttgga atggatcgcg
    46021 tcgagaaaga tctccagttg atgaagcagc acaacatcaa ctccgtgcgt accgctcact
    46081 acccgaacga tccgcgtttt tacgaactgt gtgatatcta cggcctgttt gtgatggcgg
    46141 aaaccgacgt cgaatcgcac ggctttgcta atgtcggcga tatcagccgt attaccgacg
    46201 atccgcagtg ggaaaaagtc tacgtcgagc gcattgttcg ccatattcac gcgcagaaaa
    46261 accatccgtc gatcatcatc tggtcgctgg gcaatgaatc cggctatggc tgtaacatcc
    46321 gcgcgatgta ccacgcagcg aaggcgctgg atgacacgcg actggtgcat tacgaagaag
    46381 atcgcgatgc tgaagtggtc gatattattt ccaccatgta cacccgcgtg ccgctgatga
    46441 atgagtttgg tgaatacccg catccgaagc cgcgcatcat ctgtgaatat gctcatgcga
    46501 tggggaacgg accgggcggg ctgacggagt accagaacgt cttctataag cacgattgta
    46561 ttcagggaca ttatgtttgg gagtggtgcg accacgggat ccaggcgcag gatgacaacg
    46621 gcaatgtctg gtataaattc ggcggcgact acggcgacta tcccaacaac tataacttct
    46681 gtcttgatgg tttgatctat tccgatcaga cgccgggacc aggcctgaaa gagtacaaac
    46741 aggttatcgc gccggtaaaa atccacgcgc tggatctgac tcgcggcgag ctgaaagtcg
    46801 aaaataaact gtggtttacc acgctggatg actacaccct gcacgcagag gtgcgcgccg
    46861 aaggtgaaac gctcgcgacg caacagatta aactgcgcga cgttgcgccg aacagcgaag
    46921 cccccttgca gatcacgctg ccgcagctgg acgcccgcga agcgttcctc aacattacgg
    46981 tgaccaaaga ttcccgcacc cgctacagcg aagccgggca ttctatcgcc acttatcagt
    47041 tcccgctgaa ggaaaacacc gcgcagcctg tacctttcgc accgaataac gcccgtccgc
    47101 tgacgctgga agacgatcgt ttgagctgta ctgttcgcgg ctacaacttt gcgatcacct
    47161 tctcaaaaac gagtggtaaa ccgacatcct ggcaggtaaa tggcgaatcg ctgctgaccc
    47221 gcgagccaaa gatcaacttc ttcaagccaa tgatcgacaa ccacaagcag gagtacgaag
    47281 ggttgtggca gccgaatcat ttgcagatca tgcaggaaca tctgcgcgac tttgccgtag
    47341 aacagagcga tggtgaagtg ttgatcatca gccgcacggt tatagcaccg ccggtgtttg
    47401 acttcgggat gcgctgtacc tacatctggc gcatcgctgc agatggccag gttaacgtgg
    47461 cgctttccgg cgagcgttac ggcgactatc cgcacatcat tccgtgcatc ggtttcacca
    47521 tggggattaa cggcgaatac gatcaggtgg cgtattacgg tcgtggaccg ggcgaaaact
    47581 acgccgacag ccagcaggct aacatcatcg atatctggcg cagcaccgtc gatgccatat
    47641 tcgagaacta tcccttcccg cagaacaacg gcaaccgtca gcatgtccgc tggacggcac
    47701 tgactaaccg ccacggcaac ggtctgctgg tggttccgca gcgcccaatt aacttcagcg
    47761 cctggcacta tacccaggaa aacatccacg ctgcccagca ctgtaacgag ctgcagcgca
    47821 gtgatgacat caccctgaat ctcgaccacc agctgcttgg cctcggctcc aattcctggg
    47881 gcagcgaggt gctggactcc tggcgcgtct ggttccgtga cttcagctac ggctttacgt
    47941 tgctgccggt ttctggcgga gaagctaccg cgcaaagcct ggcgtcgtat gagttcggcg
    48001 cagggttctt ttccacaaat ttgcacagcg agaataagca atgaggatca tcgataactt
    48061 agaacagttc cgccagattt acgcctctgg taagaagtgg caacgctgcg ttgaagcgat
    48121 tgaaaatatc gacaacattc agcctggcgt cgcccactcc atcggtgact cattgactta
    48181 ccgcgtggag acagactccg cgaccgatgc gctatttacc gggcatcgac gctattttga
    48241 agtgcattac tacctgcaag ggcagcaaaa aattgaatat gcgccgaaag agacattaca
    48301 ggtcgtggaa tattatcgtg atgaaactga ccgtgaatat ttaaaaggct gcggagaaac
    48361 cgttgaggtc gacgaagggc aaatcgttat ttgcgatatc catgaagcat atcggtttat
    48421 ctgcaataac gcggtcaaaa aagtagttct caaagtcacc atcgaagatg gttatttcca
    48481 taacaaataa caactacggc ggcaaaagga gtttgccgcc accgctaccc tactcatttt
    48541 cggagatgtg ttatgtctga taccaaacgt aatacaatcg gcaaattcgg cttgctctcg
    48601 ctgacttttg ccgccgttta cagctttaac aacgttatca ataataatat tgagcttgga
    48661 ctggcctcgg caccgatgtt tttcctcgcg acgatttttt attttattcc cttctgtctg
    48721 atcatcgcag aatttgtttc gttaaataaa aactcagaag ccggtgtcta cgcgtgggta
    48781 aaaagttcgc tgggcggacg ttgggcattt attactgcct atacctactg gttcgtaaac
    48841 ctgttctttt tcacctcgct gttgccgcgc gttattgctt atgcttcgta tgccttcctc
    48901 ggctatgaat atattatgac gccggttgcc accaccatta tcagtatggt gctgttcgcc
    48961 ttctccacct gggtttccac caacggggcg aaaatgttgg ggccaattac ctccgtcact
    49021 tcaacgctga tgctgctgtt aacgctctcc tacattttac tggcaggtac ggcgctggtt
    49081 ggcggcgtac agcctgctga ccccatcacc gttgacgcga tgatcccgaa cttcaactgg
    49141 gcgttcctcg gcgttaccac ctggatcttt atggccgcag gtggcgcgga gtccgtcgct
    49201 gtgtacgtta acgacgtcaa aggcggttcg aaatcgttcg ttaaagtgat catcctcgcc
    49261 gggattttta tcggtgtact atattccgtc tcctcggtgc tgattaacgt cttcgtcagc
    49321 agcaaagagt tgaaatttac tggcggatcg gtgcaggtat tccacggcat ggcggcgtat
    49381 tttggtctgc cggaagcgtt gatgaatcgc tttgtcggtc tggtgtcctt taccgcaatg
    49441 ttcggttccc tgctgatgtg gaccgcaacg ccggtgaaaa ttttcttctc cgaaatcccg
    49501 gaaggcatct ttggtaagaa aaccgtcgaa cttaacgaaa acggcgttcc ggcgcgcgca
    49561 gcgtggatcc agttcctgat cgtcatcccg ctgatgatta tcccgatgct cggttccaat
    49621 actgtgcagg atctgatgaa tactattatt aatatgaccg ccgcagcgtc catgcttccg
    49681 ccgttattca tcatgctggc ttacctgaat ttacgcgcca aattagatca cctgccacgc
    49741 gatttccgta tgggctcccg ccgcaccggt attatcgttg tttcaatgct gattgcgata
    49801 tttgccgtag ggtttgtcgc ttcgacattc ccgactggcg cgaatattct gaccatcatt
    49861 ttttataacg tcggcggtat tgttatattc cttggctttg cgtggtggaa atacagtaaa
    49921 tatataaagg gattaacggc tgaagagcgc catattgaag cgacgccagc cagcaatgtt
    49981 gattaaacag actaataaaa aatgagcagg cattttccct cttcctggat gaggagaggg
    50041 aaaatagttt ctgcctttat atttttataa ttacaacgat aaaaggctgt actttttctt
    50101 tagctcatgg attaacacaa tgaaattaat cactgcacca tgcagagcat tacttgctct
    50161 gccgttttgc tacgcctttt ctgcggcagg agaagaagca cgtccggcag aacatgacga
    50221 cacaaaaacc cccgcaatta cctcgacatc ttctccttca tttcgttttt acggcgaatt
    50281 aggggttggt ggatatatgg atttagaggg tgagaataaa cataaataca gcgacggcac
    50341 ctatattgaa ggtggcctgg agatgaagta cggctcctgg ttcggcctga tttacggcga
    50401 aggctggacc gtgcaggccg accacgacgg caatgcctgg gtgcctgacc atagctgggg
    50461 cggtttcgaa ggtgggatta accgtttcta tggcggttat cgaaccaatg atggcaccga
    50521 aatcatgctc agtctgcgtc aggattcctc gctggatgac ctgcaatggt ggggcgactt
    50581 cacacccgat ctgggctacg tcattcccaa tacccgcgac attatgaccg cgctgaaggt
    50641 gcagaactta agcggcaact ttcgttatag cgtcaccgcc acacctgccg gacatcatga
    50701 cgaaagcaaa gcatggctac attttggcaa gtacgatcgc tacgacgaca aatacaccta
    50761 tccggcaatg atgaacggtt acatccagta tgaccttgcc gaaggcatca cctggatgaa
    50821 tggtctggaa attaccgacg gcacaggaca actctatctc acgggactgc ttacccctaa
    50881 ctttgccgct cgcgcctggc accataccgg acgtgccgac gggctggacg taccgggaag
    50941 tgaaagcggg atgatggtga gcgccatgta tgaagcgtta aagggcgttt atctctccac
    51001 cgcttacacc tacgccaaac atcgccctga ccacgctgac gatgaaacca cctctttcat
    51061 gcagtttggt atctggtacg aatatggcgg cggacgtttc gcaacggctt ttgatagccg
    51121 cttctacatg aaaaatgcct ctaacgatcc cagcgaccaa atcttcctga tgcaatattt
    51181 ctactggtaa taaggactgt aatttcatga aaatcaaaac tattttaacg ccagtaacct
    51241 gcgctctgct gataagtttt tccgcccatg ccactaacgc cgacaattat aaaaacgtga
    51301 ttaaccgtac tggtgcgccg cagtacatga aggattacga ttacgacgat caccagcgtt
    51361 ttaatccgtt tttcgatctc ggagcctggc atggtcatct gttgccagac ggcccgaaca
    51421 ccatgggcgg ctttccgggc gttgcgctgc tgacggaaga atacatcaac tttatggcca
    51481 gcaatttcga ccgcctgacc gtctggcagg acggcaagaa agtcaacttc acgctggagg
    51541 catacaatat tcccggcgcg ctggtgcaaa aactgacagc aaaagatgtg caggtcgaaa
    51601 tgactctgcg cttcgccacg ccgcgcacgt cactactgga aactaaaatc atcagcaata
    51661 aaccgctgga tctggtgtgg gatggcgaac tgctggaaaa actggaagcg aaagaaggga
    51721 aaccgctttc cgataaaacc attgctggcg aataccctga ctaccagcgc aaaatcagcg
    51781 ccacccgtga tggcctgaaa gtcacctttg gcaaagtgcg cgccacctgg gatctgctga
    51841 cctccggtga atcagaatat caggtgcata aatccctgcc ggtgcagact gaaatcaacg
    51901 gcaatcgctt taccagtaag gcgcatatca acggttcgac gacgctctat accacctatt
    51961 cccatctgct gacctctcag gaagttagca aagagcaaat gcagatccgc gatattctgg
    52021 cgcgtccggc gttttatctc accgcctcgc agcaacgctg ggaagagtat ctgaagaaag
    52081 ggttaaccaa tccggatgcg acgccggaac agacacgcgt cgcggtgaaa gcgatcgaaa
    52141 cgctcaacgg taactggcgc tcgcctgggg gtgcggtgaa atataacacc gtcacgccgt
    52201 cggtgaccgg gcgctggttc tccggcaatc agacctggcc gtgggatacc tggaagcagg
    52261 cgtttgcgat ggcgcatttc aatccggaca tcgccaaaga gaatatccgc gcggtcttct
    52321 cctggcagat ccagcctggc gacagcgtgc gtccgcagga tgtgggcttt gtccccgacc
    52381 tgatagcgtg gaatcttagc cccgagcgtg gtggcgatgg tggcaactgg aacgaacgaa
    52441 ataccaaacc cagccttgcc gcctggtcgg tgatggaagt gtacaacgtc acccaggata
    52501 aaacctgggt ggcagagatg tacccgaaac tggtggccta tcacgactgg tggttacgta
    52561 accgcgatca caacggcaac ggcgtgccgg aatatggcgc gacccgcgac aaagcccaca
    52621 acactgagag cggcgagatg ctgtttacgg tgaagaaagg caacaaagaa gagacgcagt
    52681 ctgggctgaa caactacgcc cgcgtggtgg agaaaggcca gtacgacagt ctggaaattc
    52741 cggcacaggt tgctgcatcg tgggaatcgg ggcgtgatga tgccgccgtc tttgggttta
    52801 tcgacaaaga acagctggat aaatatgtcg caagcggcgg caaacgtagc gactggacag
    52861 tgaaattcgc cgaaaaccgc agtcaggacg gaacgttgct ggggtactcg ctattgcagg
    52921 agtcggtgga tcaggccagc tatatgtaca gcgataacca ttatctggcg gagatggcga
    52981 cgattctcgg taaaccggaa gaggctaagc gctatcgcca gttggcacag cagctcgcgg
    53041 actacatcaa cacctgtatg ttcgacccgg ctacgcagta cttctatgac gtgcgtattg
    53101 aagataaacc gctggcgaac ggctgcgcgg gcaaaccgat tgttgagcgc ggtaaagggc
    53161 cggaaggctg gtcgccgctg tttaacggtg cggcaacgca ggccaatgcc gacgcggtgg
    53221 tgaaggtgat gctcgatcct aaagagttca atacctttgt tccgctggga acggcggcgt
    53281 taaccaatcc ggcctttggc gctgatatct actggcgcgg gcgcgtatgg gtggatcagt
    53341 tctggtttgg tctgaaaggg atggagcgtt acggttatcg cgatgatgcc ctgaaactgg
    53401 cggatacgtt cttccggcac gccaaagggt taaccgccga tggcccaatt caggaaaatt
    53461 acaacccgct gacaggcgca cagcaaggcg caccaaattt ctcctggagt gccgcacatt
    53521 tttatatgtt gtataacgat tttttccgta agcaataatt tgcaacggct ggcggatgcg
    53581 gcgtgaacgc cttatccgcc ctacatattc gttcccgtag gctggataag acgcgacaag
    53641 cgtcgcatcc ggcatctgca cctcgccacc ggcggatgct gcgtgaacgc cttatccgcc
    53701 ctacatgttc gttcccgtag gctggataag acgcgacaag cgtcgcatcc ggcatctgca
    53761 cctcgccact ggcggatgcg gcgtgaacgc cttatccgcc ctacatgttc gttcccgtag
    53821 gctggataag acccgacaag cgtcgcatcc ggcgtctgca ccgcgccact ggcggatgcg
    53881 gcgtgaacgc cttatccgcc ctacatgtgc aatcccccag gtcggatagg gcccggcaag
    53941 cgtcgcatcc ggcgttacca ccgggcgtat tctttttgaa tcccatcaca aaccccgcac
    54001 tccccttttc ccttttctcc ggcgacggct aaattagaac tcatccgacc acataacaat
    54061 tattttacat actggacaat tttatgagct acccgtcgct gttcgccccg ctggatttag
    54121 gttttaccac gttaaaaaac cgcgtgttga tgggttcaat gcacaccggg ctggaggaat
    54181 acccggacgg tgctgaacgg ctggcagcgt tttatgccga acgcgcccgt cacggcgtgg
    54241 cgctgattgt tagcggcggc attgcaccag atttaacagg cgttggcatg gaaggcggcg
    54301 caatgctcaa tgacgccagc cagatcccac accatcgcac cattaccgaa gcggtacatc
    54361 aggaaggcgg caaaatagcc ctgcaaattt tgcataccgg gcgctacagc taccaaccgc
    54421 atctggtcgc accgtccgca ttgcaggccc ccatcaaccg tttcgttcct catgaactca
    54481 cccatgaaga gatcctgcaa ctgatcgacg atttcgcccg ctgcgcgcaa ctggcacggg
    54541 aggcaggata cgacggtgta gaggtaatgg gttccgaagg gtatttgatc aacgaatttc
    54601 tgacgctgcg caccaatcag cgcagtgacc agtggggcgg cgattaccac aaccggatgc
    54661 ggtttgccgt tgaagtggtg agtgcggtac gcgaacgcgt tggcaacgac ttcattatta
    54721 tctaccgact gtcgatgctc gacctggtcg aaggcggcgg gacttttgcc gaaacggtag
    54781 agctggcgca ggccattgaa gcggcgggcg cgaccattat caacaccggc attggctggc
    54841 atgaagcgcg tattccgacc atcgccacgc ccgtgccacg cggcgcattt agctgggtca
    54901 cgcgcaaact gaaaggtcac gtctcgctgc cgctggtgac caccaaccgg attaacgatc
    54961 cgcaggttgc cgacgatatt ctctcgcgcg gcgatgccga tatggtatcg atggcgcgac
    55021 cgtttcttgc ggatgcggaa ctgctgtcaa aagcgcaatc agggcgagcc gatgagatca
    55081 acacctgcat tggctgcaac caggcgtgtc tcgatcagat cttcgttggc aaggtcacct
    55141 cgtgtctggt gaatcctcgc gcctgccatg aaaccaaaat gccgatcctt cccgccatgc
    55201 agaaaaaaaa tctggcggtg gtcggtgcgg gacctgctgg gctggcgttt gccattaacg
    55261 cggcggcgcg tggtcatcag gtaacgttgt ttgacgcgca cagtgagatc ggcgggcagt
    55321 ttaatatcgc caaacagatc cccggcaaag aggagtttta cgaaacgctg cgctattacc
    55381 gccggatgat cgaagtgacg ggcgtgacgc taaaactcaa tcacaccgtg acggcggatc
    55441 agttacaggc gttcgatgaa acgatcctcg ccagtgggat cgtgccgcgc actccgccca
    55501 tcgacgggat cgatcatccg aaggtattga gttatctcga tgtactgcgc gacaaagcgc
    55561 cggtgggcaa caaagttgcc atcatcggtt gtggcgggat tggttttgat acggcgatgt
    55621 atttaagtca gccgggcgaa tccaccagcc agaatatcgc cgggttctgt aatgaatggg
    55681 ggatcgacag cagcctacaa caggctggtg gcttaagccc gcagggaatg cagatcccgc
    55741 gtagcccacg gcagattgtg atgctccagc gcaaagccag caaaccggga caggggttag
    55801 gcaaaaccac cggctggatc catcgcacca ccctgctctc gcggggtgtg aaaatgatcc
    55861 caggcgtaag ttatcagaag attgacgatg acgggctgca tgtggtgatc aacggcgaaa
    55921 cgcaggtatt agcagtggac aatgtggtga tctgcgcagg gcaagagcca aaccgcgcat
    55981 tggcgcagcc gctgattgat agcgggaaaa cggtgcattt aattggcggc tgcgatgtgg
    56041 ctatggagct ggacgcacga cgggcaattg cccagggaac acggctggcg ctggagattt
    56101 aaatcgtg
//
LOCUS       DAZFAB010000021        54433 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZFAB010000021 DAZFAB010000000
VERSION     DAZFAB010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 54433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54433
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.021"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(83..2395)
                     /gene="pbpC"
                     /locus_tag="ACHJWA_001462"
     CDS             complement(83..2395)
                     /gene="pbpC"
                     /locus_tag="ACHJWA_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311412.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFO4308093.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEVA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEALPLPDGALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGEIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIASVHFSFQ"
     gene            complement(2396..7357)
                     /gene="yfhM"
                     /locus_tag="ACHJWA_001463"
     CDS             complement(2396..7357)
                     /gene="yfhM"
                     /locus_tag="ACHJWA_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFO4308094.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSAPERQKLAQQSAGKALTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPNQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKAGYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADEFGSNESKVIVAAPVIAELNMPRF
                     MASGDNSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRDEEAIALALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYSLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKAQNSNLNSDQLATLQVTNSGEQPLWLRVDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            7564..8409
                     /gene="sseA"
                     /locus_tag="ACHJWA_001464"
     CDS             7564..8409
                     /gene="sseA"
                     /locus_tag="ACHJWA_001464"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFO4308095.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPLK"
     gene            8752..8877
                     /gene="timP"
                     /locus_tag="ACHJWA_001465"
     CDS             8752..8877
                     /gene="timP"
                     /locus_tag="ACHJWA_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFO4308096.1"
                     /translation="MKIRCFCIVLIVSGALLTEVNNNRSLSGDNLLVINNLQSSR"
     gene            complement(8909..9685)
                     /gene="sseB"
                     /locus_tag="ACHJWA_001466"
     CDS             complement(8909..9685)
                     /gene="sseB"
                     /locus_tag="ACHJWA_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFO4308097.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQVAGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(9828..11111)
                     /gene="pepB"
                     /locus_tag="ACHJWA_001467"
     CDS             complement(9828..11111)
                     /gene="pepB"
                     /locus_tag="ACHJWA_001467"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFO4308098.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVASDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(11289..11489)
                     /gene="iscX"
                     /locus_tag="ACHJWA_001468"
     CDS             complement(11289..11489)
                     /gene="iscX"
                     /locus_tag="ACHJWA_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFO4308099.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(11501..11836)
                     /gene="fdx"
                     /locus_tag="ACHJWA_001469"
     CDS             complement(11501..11836)
                     /gene="fdx"
                     /locus_tag="ACHJWA_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFO4308100.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(11838..13688)
                     /gene="hscA"
                     /locus_tag="ACHJWA_001470"
     CDS             complement(11838..13688)
                     /gene="hscA"
                     /locus_tag="ACHJWA_001470"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFO4308101.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(13705..14220)
                     /gene="hscB"
                     /locus_tag="ACHJWA_001471"
     CDS             complement(13705..14220)
                     /gene="hscB"
                     /locus_tag="ACHJWA_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFO4308102.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(14316..14639)
                     /gene="iscA"
                     /locus_tag="ACHJWA_001472"
     CDS             complement(14316..14639)
                     /gene="iscA"
                     /locus_tag="ACHJWA_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFO4308103.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(14656..15042)
                     /gene="iscU"
                     /locus_tag="ACHJWA_001473"
     CDS             complement(14656..15042)
                     /gene="iscU"
                     /locus_tag="ACHJWA_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFO4308104.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(15070..16284)
                     /gene="iscS"
                     /locus_tag="ACHJWA_001474"
     CDS             complement(15070..16284)
                     /gene="iscS"
                     /locus_tag="ACHJWA_001474"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFO4308105.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(16396..16884)
                     /gene="iscR"
                     /locus_tag="ACHJWA_001475"
     CDS             complement(16396..16884)
                     /gene="iscR"
                     /locus_tag="ACHJWA_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFO4308106.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(17185..17922)
                     /gene="trmJ"
                     /locus_tag="ACHJWA_001476"
     CDS             complement(17185..17922)
                     /gene="trmJ"
                     /locus_tag="ACHJWA_001476"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFO4308107.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            18041..18844
                     /gene="suhB"
                     /locus_tag="ACHJWA_001477"
     CDS             18041..18844
                     /gene="suhB"
                     /locus_tag="ACHJWA_001477"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFO4308108.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            18989..19843
                     /locus_tag="ACHJWA_001478"
     CDS             18989..19843
                     /locus_tag="ACHJWA_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO4308109.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVNFI
                     LSALNPQN"
     gene            20034..21314
                     /gene="csiE"
                     /locus_tag="ACHJWA_001479"
     CDS             20034..21314
                     /gene="csiE"
                     /locus_tag="ACHJWA_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFO4308110.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(21306..22445)
                     /locus_tag="ACHJWA_001480"
     CDS             complement(21306..22445)
                     /locus_tag="ACHJWA_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFO4308111.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(22605..23495)
                     /gene="hcaR"
                     /locus_tag="ACHJWA_001481"
     CDS             complement(22605..23495)
                     /gene="hcaR"
                     /locus_tag="ACHJWA_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFO4308112.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            23631..24992
                     /gene="hcaE"
                     /locus_tag="ACHJWA_001482"
     CDS             23631..24992
                     /gene="hcaE"
                     /locus_tag="ACHJWA_001482"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFO4308113.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            24989..25507
                     /gene="hcaF"
                     /locus_tag="ACHJWA_001483"
     CDS             24989..25507
                     /gene="hcaF"
                     /locus_tag="ACHJWA_001483"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFO4308114.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEICYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            25507..25827
                     /locus_tag="ACHJWA_001484"
     CDS             25507..25827
                     /locus_tag="ACHJWA_001484"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFO4308115.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            25824..26636
                     /gene="hcaB"
                     /locus_tag="ACHJWA_001485"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             25824..26636
                     /gene="hcaB"
                     /locus_tag="ACHJWA_001485"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFO4308116.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIAGEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            26646..27848
                     /gene="hcaD"
                     /locus_tag="ACHJWA_001486"
     CDS             26646..27848
                     /gene="hcaD"
                     /locus_tag="ACHJWA_001486"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFO4308117.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDEQHLPYER
                     PPLSKSMLLEDSPQLQSVLPAHWWQENNVHLHSGVTIKTLGRDTRELVLANGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANDQLAREANLDTTNGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNHAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRSIRKWIQSGKTFDAKQLTD
                     ENIALKSL"
     gene            27945..28367
                     /locus_tag="ACHJWA_001487"
     CDS             27945..28367
                     /locus_tag="ACHJWA_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO4308118.1"
                     /translation="MNSLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(28415..29287)
                     /locus_tag="ACHJWA_001488"
     CDS             complement(28415..29287)
                     /locus_tag="ACHJWA_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFO4308119.1"
                     /translation="MTIYTLSHGSLKLGVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIIETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(29299..30393)
                     /locus_tag="ACHJWA_001489"
                     /pseudo
     CDS             complement(29299..30393)
                     /locus_tag="ACHJWA_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
     gene            complement(30426..31424)
                     /locus_tag="ACHJWA_001490"
     CDS             complement(30426..31424)
                     /locus_tag="ACHJWA_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4308120.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSHHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(31449..32960)
                     /locus_tag="ACHJWA_001491"
     CDS             complement(31449..32960)
                     /locus_tag="ACHJWA_001491"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO4308121.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGEIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAHKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVIRAVKKMSALGVAVIYVSHRMEEIRRIASS
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHAPVNVDELMSAILSVH"
     gene            complement(32983..33966)
                     /locus_tag="ACHJWA_001492"
     CDS             complement(32983..33966)
                     /locus_tag="ACHJWA_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFO4308122.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(34063..37344)
                     /locus_tag="ACHJWA_001493"
     CDS             complement(34063..37344)
                     /locus_tag="ACHJWA_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HFO4308123.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTRLDAYEE
                     KRFEQFFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCD
                     ALLDDAVALTPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDVAKA
                     PLSAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPINQEVLCLHNLLLVLSGRQDNARL
                     QREKLLRDYPLNATLWCLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNEAIALWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGTTPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICAKAQGDETEATRCLHLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQHTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLAAAGLGEIAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            37462..38655
                     /locus_tag="ACHJWA_001494"
     CDS             37462..38655
                     /locus_tag="ACHJWA_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFO4308124.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGEHWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVET
                     QLRQI"
     gene            complement(38718..39971)
                     /gene="glyA"
                     /locus_tag="ACHJWA_001495"
     CDS             complement(38718..39971)
                     /gene="glyA"
                     /locus_tag="ACHJWA_001495"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFO4308125.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEVEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            40299..41489
                     /gene="hmpA"
                     /locus_tag="ACHJWA_001496"
     CDS             40299..41489
                     /gene="hmpA"
                     /locus_tag="ACHJWA_001496"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFO4308126.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFAAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(41534..41872)
                     /gene="glnB"
                     /locus_tag="ACHJWA_001497"
     CDS             complement(41534..41872)
                     /gene="glnB"
                     /locus_tag="ACHJWA_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFO4308127.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(41933..43267)
                     /gene="glrR"
                     /locus_tag="ACHJWA_001498"
     CDS             complement(41933..43267)
                     /gene="glrR"
                     /locus_tag="ACHJWA_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFO4308128.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(43257..43970)
                     /gene="qseG"
                     /locus_tag="ACHJWA_001499"
     CDS             complement(43257..43970)
                     /gene="qseG"
                     /locus_tag="ACHJWA_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFO4308129.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(44135..45562)
                     /gene="qseE"
                     /locus_tag="ACHJWA_001500"
     CDS             complement(44135..45562)
                     /gene="qseE"
                     /locus_tag="ACHJWA_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFO4308130.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELDNVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(46138..50025)
                     /gene="purL"
                     /locus_tag="ACHJWA_001501"
                     /gene_synonym="purI"
     CDS             complement(46138..50025)
                     /gene="purL"
                     /locus_tag="ACHJWA_001501"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFO4308131.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIATLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            50282..51838
                     /gene="mltF"
                     /locus_tag="ACHJWA_001502"
     CDS             50282..51838
                     /gene="mltF"
                     /locus_tag="ACHJWA_001502"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFO4308132.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHMQ
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(51835..52338)
                     /gene="tadA"
                     /locus_tag="ACHJWA_001503"
     CDS             complement(51835..52338)
                     /gene="tadA"
                     /locus_tag="ACHJWA_001503"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFO4308133.1"
                     /translation="MSEVEFSHEYWMRHAMTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(52396..53031)
                     /gene="yfhb"
                     /locus_tag="ACHJWA_001504"
     CDS             complement(52396..53031)
                     /gene="yfhb"
                     /locus_tag="ACHJWA_001504"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFO4308134.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQALQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            53240..54088
                     /locus_tag="ACHJWA_001505"
     CDS             53240..54088
                     /locus_tag="ACHJWA_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFO4308135.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            54144..54404
                     /gene="yfhL"
                     /locus_tag="ACHJWA_001506"
     CDS             54144..54404
                     /gene="yfhL"
                     /locus_tag="ACHJWA_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFO4308136.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPQHVETEEQLWDKFVLMHHADKI"
BASE COUNT        12854 a        15041 c        13760 g        12778 t
ORIGIN      
        1 ttaaaaaaac gtgagattat taaattatta atgcattcca aatataaata ccataattag
       61 attaataaat attttgtata tttcactgaa atgaaaaatg tactgacgcg atttgcccta
      121 cgtcatccat caccagcaat tgataatcac ctttatccgt caaatgcagg gtcacgttgc
      181 gcccgcgttc agttaacggt tcgccattca gaaaccacca gcgttcacct gcccctccac
      241 ttgattgcaa cggcaaagtc gcttccgctg cgcccggtaa acgtttaata atctcgccat
      301 cgcgaacgcc tgtcagctgc agcgggagct gggcatcgtg accgtatggc gggcaaattg
      361 tcgaggccgg tggtaagcgc acagcgcgac gttctgatgc gggcaaccag ggttccagcg
      421 gtagcggcca gacatttatc atttcttgtc gcgcttgcgg gcaatcagcg gcaacacgtt
      481 tgccattttc atccagccag atggggaaac gaatgccatt aatgccttcc tgctccggca
      541 gtaatagagt tggcggctga ctcccgtcca gcagccaggt tgccaggcgg cggcgacagt
      601 taccgtcacc ttccggcaaa gactgtccgc ccggccagca aataacgcca cgagtgactg
      661 agttcgggcg cgggtcttcc ggcagattag cactgcgcga cagtaagata ttattgacct
      721 gattcaacaa tggtacggcg ctggcaaaac caaactgacc aacaacgggc gtgccgtccg
      781 gtctgccagt ccagatccca atgacatagc gagcgttaac cccaatcgcc caggcgtcac
      841 gatagccata gctggtgccc gttttccatg ccagtggggc gacgcgcggc aaggcaccat
      901 ccggcaaggg tagcgcttca tcagccataa tccggcgaat gatccacgcc gctcccgacg
      961 acattaaagg ccgttcaagc agcggatcgt caggctgtaa gcgcaatttg cctgccttgc
     1021 cgtggcgagc aaacgcggta tacgctgccg ccatatcttc cagtttcgca ccagcgccgc
     1081 cgagaatgag cgaaagattc ggcgcagcac cgttgggcaa atataacggc aatccaacat
     1141 tgcgtaactt tgccgcaaac cgtttcggtc cataggcttc cagcacctgc acagcaggta
     1201 aattcagcga gcgcaccagc gcctcgctca tgctgatcgg gccatgaaaa ccgctgtcaa
     1261 agttgccagg tcggtaatca ccggtacgcc gggggacgtc ttgcagcagt gatgccgggt
     1321 ggatcaatcc ttcatccagc gccagaccgt aaacaaacgg tttgagcacc gatcctggcg
     1381 atcggatcgc attgaccata tcaacatgac caaagcgtga atcatcgttg agatcaaccg
     1441 aacccaccca gccgcgaaca cgcatatcgg tatgatcaac cacgatcatc gccagtgagc
     1501 tgcgcggtgg taatcgtcct ttccagtttt gcgccagttc ttccagacgt cgttgaagac
     1561 cggcatccag cgtagtaacg attttgtcgc ttttgctttt accgagcatc atgcgcgaaa
     1621 acagcggtgc cagttgcggc atttgtcggg gtgccagcca gatgggttct tcccttgact
     1681 cttttacctg ctcacgggac cacacacctt gcacggccat ccgttcgagc actttattac
     1741 gcgcgacttc ggcacgctcc ggccaacgat ccgggcgaag acggctgggc gcttgcggca
     1801 aaaccgccag cattgccgcc tcggaatagc ttaaattcgc aggcgacttt ccgagataag
     1861 cccaacttgc cgcaccgatc ccctgcaacg taccgccaaa cggagcgcgg ttaagataca
     1921 aggtcagaat ttcacgctta gacagatgcc attccagttg caacgcgcgc cagagctggc
     1981 gaattttgcc gccaaatgtt ttggggtgag gatcaagcag acgagcaacc tgcatagtga
     2041 gcgtgctgcc accggaaata acccgtcccg aagtgagatc ttgccatgct gcgcgcgcca
     2101 ccgagaatgg attcacccca ggatgcttcc agaaccagcg atcttcataa ttgatcagcg
     2161 cttcaaggta acgtggagaa acatcttcga ttgttacagg ataacgccag atgccgtcag
     2221 catcggcgaa gcgccagagc ggcgtaccat cctgcgccac gaccactcgt gcgggattga
     2281 cttcatgcag cggtagaggc cagagtttat ctgctcccca cgctgcaaga aaaaggagaa
     2341 atggcgcagc tgccagcgtt atccagcagc cgcgtttggt taacaagcga ggcatttacg
     2401 gtctgacaat cagcaaatct tcagccgcgc cggtcgcccg ccattgggga acatacattg
     2461 attccaccat cggttgcggt acctgatacg ttcccggcgt caccgcccgc gccagataca
     2521 ccagcgttac cggttggtat tcatcaacgg caaccgccgc cacaaagcga tcgtcacgga
     2581 actcaatgtg cttaatactc gcctgctgca tctggttcag taagttctgc acttcgccac
     2641 cactttgctc caggctggcg ctaccgttcg ccagattctg gttttccagt tccagacccg
     2701 caggcagcag atccacgact aacgcatccg gcacgctgtt actagctttt acctgcaacc
     2761 agaccagcac cagatcgccg ctacgtaacg agtccagcga tttgctctta ccatcagtac
     2821 caagaatatg acgctcaatt tgcagcacat tgttcgccgg taaaggtgcg gattgcggat
     2881 aaccgctggc atccacacgc agccataacg gctgctcgcc actgttggtc acctgcaagg
     2941 tggcaagttg atcgctattc agattgctgt tttgcgcttt ctcgcctgtc agcggctcag
     3001 ctgagaaaga ggtttgcgcc tgccatttac ctggtaaatc ctgaatcgta cgggcagcca
     3061 ggaacaacgc gttactttcc tgcgtcgata gccagcgttc accaaacgcc tgctgcgaaa
     3121 gtgtgttcag cagggagtac tgctcatcgg gtagcagttt gttctcttcc agcaaggaga
     3181 gcatcaacgc gttgtcgcgc agtggactac cgtaatcacc cagccatatc cgctcatcac
     3241 tattacgcgg cgtttttagc gccagcgcaa tcgcttcttc atcacgcgtc gcatcaccca
     3301 tggttttcag cgcaacgcca agttgcagca gcggtaaacc agaagcggca tctgcacgat
     3361 gctcccagat ttcacgcagc gcacccagcg gagccttttg ctgacgggcc aacaccagcg
     3421 cagcgtaaga ctgtacggcg aatttactgg ctttgagatt atccgcgtac gggatcgaca
     3481 tcatgcccgg atcttgtaaa tagcgcagca gacgctcatt accccggtta atggcgtctg
     3541 tcggcacgct gtaaccctgt tcgcctgcgc ggaccaggaa atccatcacg taagccgtca
     3601 gccagtactc ttcgtcaccg tttttatccc acagcgcaaa gccgccgtta tcacgttgca
     3661 tttgcagcaa acgggaaatg ccgatatcga ccgatgcacg gcgtttctca tcactgtcgc
     3721 ctttgatgcc caacgcctgc agttgggctg cgttggtata aagtgacggg aacaggccgc
     3781 tggcggtttg ctcaagacag ccgtacggat acgcttttaa ctctttgata taacgtgcga
     3841 tgttcagcgg tggtttgccg ctcaacaaca attgcccttc cagcgtaaca ggcgagaagt
     3901 tttgcaatcc atccgccgga atcgcccatg tctcaccagg ctgtaacgcc gtaccgtaat
     3961 taaccgtttg tgccgggaac gccggacgga cgccgatttt ccactgctta tgctgatcgg
     4021 caacggtttc acccggtaac gctaacccgc taatggtggc ctgaatttct ccatcgccat
     4081 aacccggcaa tgctcgcacc gggataaaca gcgtagtacg cacacctggc gctaattcaa
     4141 cggctgcggg tgaatcgctg accagttcaa gcaaaccact ggcggtcagg gcaacgttca
     4201 gtttttgcgg tttatcggta agattagtga tatccagcgt cagacgcgag ttatcgccac
     4261 tcgccataaa gcgcggcatg ttcagttcag caatcaccgg tgcggcaact atcactttgc
     4321 tttcgttgct accgaattca tctgccgtcc aggcttgcgc catgacgcgc aattcaccgt
     4381 taaaatcgcc aatcggcagt gtaaccgagc cttcgccctg ttcgttgagc gttaccggca
     4441 gcgcctgctg cgcgacaata ttgacgtgat tgaccggcgg tttaccacca cgtttcagct
     4501 catcaccatc gccaccgaaa cgcagagctg ccagacgccc ctgaccttca ataacctgac
     4561 cgtaaatatc gtaaatgtct gcgccatagc gtttctgacc aaagaacgcc tgccacggat
     4621 ctggcgtgac gtagtcagta atattcagca caccactatc aacggcagac accagcacat
     4681 tcacctgttt aggcttctcg ccatttttag tgctggcttt aattttcacg gttaatggtt
     4741 gattgggacg catttttgct ggtgtttcca gcgccagatc gaggcgacgg ttttcatcac
     4801 caagcggcag atgcaacaca ccaaccgcgc gttttggcgt cgcggagcga gatttatcgc
     4861 caggacgcac caccagcgta ctcaaataaa gatcatgacg attccaggtt ttatcgaccg
     4921 gaatcgtcag atccagccct tgagccggaa catcaatctc ttgccaccac agcggccctt
     4981 cactggactc gaccatcgca taacctttac ccgccgttgg cgcggcgata tgcaacttaa
     5041 tggtatcgcc agggcgataa ccggctttat ccagtttcag cgtgacacgg tcgggtcgca
     5101 ctgcgccgct accgtcgcta ttatcctgcc agctatagcc agcccaaaaa cgaacactac
     5161 tgaccgcttc attcggcgct ttgacttcca gacgataagc gccccactct accgggaaac
     5221 tgaccttgcc ggtttcgtcc gctttcagat ccagagtttg ttcattttca atcagatctt
     5281 tttgatcaaa ctgtgattgc cagccttcat cttctgacca gttccagtag taatcgcggc
     5341 gttcgcgaat caggcgcacc tgcaagcccg acacggcttt tttcacgcct tgcgcatcgc
     5401 tataaacgat gtcaaaagcg gcgttactgc cttcatcaac aatgggctgt ttcaccgtgc
     5461 tgtcagtgcg ataatcgtaa accgatttcg aggcgaactg cggacggatc cccggcaatg
     5521 catcggcagg ccagatagcc tgctcagcgc ggcgcgtcac cgggcgaccg cccgattcca
     5581 gcaaactgcc ctggaaaata acctgcaatg gggaatgcgt ttccttccac tggctttctg
     5641 tagaaacttc gccgcgccct ttatcatcca gcgtcaactg aacttcatcc agcgtgcggg
     5701 aaaggttttc ggcagctata tcgccgaatt caaaaccagg taaggctgac acagcctcac
     5761 gcagtgggcg caggaaaagt tgcccttgca aagtattacc attagcaggc gcaccgtaca
     5821 ggtagtatcc caccacggag aatttcactt catcattcgg cgtaagcggg gttttctcac
     5881 cggtcagatt cagcgccata cgctctggca taaaatcttc gacgtggaaa tcccacatcc
     5941 gatactggtt atcgcccgtg ttagcgcgaa tatgccacat accggttgcc gcattgctat
     6001 cgagtggcca ggtaaagtgg tagaggccat tttccggctg actaacgacg ctcctgagta
     6061 cctgcccatc gggtttaatc acgtctaact tgatgggttg attgggcaac gctttaccgt
     6121 ctgcatcacg cagcaaacca ttgaggatta ccgtttcacc cgggcgataa agatcgcgcg
     6181 ggccaaacat gaaaaactgt ttgctatagc ctggcgcgcc agcaatgtta aattctgcta
     6241 agtccagcgc cggaagtttt aaatcgagta gcgttgtctg accgtcttta cgtgccagca
     6301 ataatgcggc gtttttatca ttttccagct gcacatgccc ctgagcgtca ctggttgcct
     6361 gagtcagagt ctgccctttc tcatttaata aagagacttc aattccttgc tgggccgcgc
     6421 cgttttccag actttgggta aagatatcca gacgattgtg ataacggtga gctgaaacgc
     6481 cgatatcact taacgtaaac agcgtcgcgg gattactgta atcgtaacgt ccagcctgat
     6541 tcatcacagc cagatacacg cccgcctgct gaagcggttt gatatcgccc agcggcagca
     6601 ataatttttc acgggtgtta cgcgcaggat tgagatcaaa ccgtccggtg tagaccagat
     6661 ccgccatctg cagcagtttg tctgactgcc agttcgccag cgaattgcgg tattcccatt
     6721 ggctaatgaa tgctggcaga gattctggct taacgcggaa gaagttaaca tcgacattat
     6781 taacgttgag cgccattacc ggcagccctt caacgacttt accaggcagc agcgaaccac
     6841 ggctggcaaa accgacactg ggttggatgt cgcgggtagt tatagttttt tcgtaatctt
     6901 tactgaaggt tgcgttgttg agtgctttga cctccttgcc aattgtaacg atcaaatcac
     6961 gtttcggttc gaggtggcgt aaacgcagct ctttaagatt atctgatagc tcccaggcac
     7021 catccacttt gccgcttttt ttatcgacga catgaataac gcgtgagaaa tcctgatccg
     7081 ggtcgagagg gatggagaac gtcagcacca gcgtggctgc accatcaagt tggacttcag
     7141 agagatccag caatgtcagc gccttaccgg cactctgttg ggccagtttt tgtcgctctg
     7201 gcgcggagag ttttgctgaa ctcgcgtttt ctgaagaggc cgctttagta acttcagaag
     7261 gcgcatcttt tttcaccgct gttggcgcgt tatcgttgtt gtcgcaccct gccagcgcca
     7321 gcattagcat gcaggcggct acgcgtaact ttttcatttt tcatccctgc cacaatggcc
     7381 cgttagcaac gtcgaataat tattatgcgt gagaatttac gttatgtaat tcagtatcac
     7441 gcccatacaa tccgaatttt agtgagaatt gtggctcaga cagccaaatc agcctctttt
     7501 gcttgtcgct tttcactaaa catccgacaa tttagcctaa ccccggcaaa aatggagatg
     7561 cctatgtcca cgacatggtt tgtaggagcc gactggctcg ccgaacatat tgatgacccg
     7621 gaaattcaga ttatcgatgc ccgcatggcg tcgcctggac aggaagatcg taacgttgct
     7681 caggagtatc tgaatggaca tattcccggc gcagtgtttt ttgatatcga agcgctttct
     7741 gatcacactt ccccgcttcc gcacatgctg ccgcgcccgg aaacgttcgc cgtggcgatg
     7801 cgtgaattag gcgttaacca ggataagcac ctgattgtct atgacgaagg taatcttttc
     7861 tcagccccac gagcatggtg gatgctgcgc acctttggtg tagagaaagt gtcgattctg
     7921 gggggtggac ttgcaggctg gcagcgcgat gatctgttgt tagaagaagg tgcagtagag
     7981 ctgccggaag gagagtttaa cgccgcgttt aatcctgaag cagtggtgaa agtaaccgat
     8041 gtgttattgg caagccatga aaatacggcg caaattattg atgcccgccc ggctgcacgt
     8101 tttaacgcag aagttgatga acctcgccca ggtttacgtc gcggacatat tcccggtgca
     8161 ttgaacgttc cgtggacgga actggtgcgc gaaggcgaac taaaaacgac cgatgaactg
     8221 gatgcgatat tttttggtcg cggcgtcagc tatgacaaac caattatcgt cagctgcggc
     8281 tctggtgtaa cagcagccgt ggttttgttg gcactcgcga cgctggatgt gccaaacgtg
     8341 aaactgtacg acggcgcatg gagtgaatgg ggcgcgcggg cagatttacc ggttgagcca
     8401 ttgaaataag ctttttacag gcaataaaaa accgccgaat ttggcggttt tttattgctg
     8461 gtctggttcg cggcctttcc agcaggttga cttgtgttgc cggagcaacg caggcacccc
     8521 acagtgttga aatactctct tttatgcctg gctgtgtcaa gataataaac agagtaatta
     8581 ttcaaacatg agagtattat tcttaatggc ccttttcgcc gtctcgcaaa cgggcgctgg
     8641 ctttaggaaa gaatgttcca tgaccgtaaa tacaggcacc ccacagtgct tgctatcgcg
     8701 gcaatatcgc cagtggtgct gtcgtgatgc ggtcttcgca tggaccgcac aatgaagata
     8761 cggtgcttct gtatcgtact tattgtttct ggtgcgctgt taaccgaggt aaataataac
     8821 cggagtctct ccggcgacaa tttactggtg attaacaacc ttcagagcag caggtaagcc
     8881 cgaatgccgc cctttggggc ggcatatttt agattatccg attctgttta aagtcacgca
     8941 aaaaaccacc ccagcgacgt tcatagaatg gcgcaatatg ttcggtaata aagtggctaa
     9001 ttcctttttc cccttttttc acctgacaaa tatcgattgg ttcatcgcca ggtaatgtat
     9061 cggtcgctac acttcccgcc acctgaataa tttcttcgat atcaccatcg gcttcaatgc
     9121 caataagtaa attaggctgt gcctcttcgt tctctttaat tgaacaaata aaagcacgct
     9181 tcacaggctt aatggtttta aataaggtgg tgagtgaatc aatcatttgt gctggcggct
     9241 ctgcgacttc cgataatatc agcgattcac cgccttccag gatttcctgg ctgctcagcg
     9301 gatttccctc ttcaccaatc aacaaactga tttcacgcgg cataaattct ttaccggtcg
     9361 gcagtttggc attaaggaag agcgtttcgc caagggtcat ctcaaacaat gtgcgaacgg
     9421 gcattacgac aaatgcctgt tcgtcttcaa ccgcctgttg aagtgcttct aacgaggtaa
     9481 aaaaaggaat gacgctggtg ccgtcttctt tttcccagtg ctgtaaatca agcgcgctat
     9541 cttcaaccac agcctcgccc tgcgccgccg taccaggcac ccagacggtg gattccagta
     9601 gagtacggaa aaaggccggg cggtgcgccg gttcggttgc tgctttttcc agcaggtctt
     9661 caagttcatt ttttgtttcg gacataagaa ccacaattca ttcaacgttc ggggcacaag
     9721 tgcctgatgc gccatactta tcaggcctac aagggattca caatttgttg aatttgcaat
     9781 actttgtagg ccggataagg cgtttacgcc gcatccggca tattagttta cgccgttaac
     9841 agattagcaa tcgtgcgcac accaagtccc gtagcacccg cagaccactg ttcaaccggc
     9901 gctttacggt aagtcgccga gcagtcgata tgcagccagc cttgctgata gttctcaaca
     9961 aagtgcgaca ggaagcccgc tgccgtgctc gcgcctgccg gatacgccgc gcttccggta
    10021 ttgttcagtt cggcaaagtt agacggcagc tggctgcggt ggaattccgc cagcggcaga
    10081 cgccagaatg gttcgttctc ttgtgaggca ctcgccagca aacgaccggc aagcgcatcg
    10141 tcaaaactga acagcgcgtg ataatcatta cccagcgcag ttttcgccgc cccggtgagg
    10201 gtcgccgcat caatgatcat ttccggtttc tgcgcactgg catcaatcag accatcggca
    10261 agcaccaggc gcccttcggc atcagtgttc atcacttcaa cttttttacc gttgcgataa
    10321 gtgatgatat cgcccagctt gaacgcattg ccgctaatca ggttatccgc acagcagagg
    10381 aacagcttca cgcgcttgtt cagtccgcgc gtaatggcaa atgccagcgc cccggtaacc
    10441 gttgccgcgc cgcccatgtc cgacttcatc gagtccataa acgcagtctg tttgatgctg
    10501 tagccgccgg agtcaaaagt gataccttta cctaccaggc acgcgtacac tggcgcttct
    10561 ttatcgccag ttgggttgta atccagcgcc agcaataccg gagaacgttc tgaaccgcgt
    10621 ccgacggtgt gcagccccat ataaccttgc tcacgcagat cttcgccttt ggtgatccga
    10681 taagtcacac gatcgctcgc gacgttgctg atcagatcaa cagcacgctg tgccagttgc
    10741 gatggtccca attcttctgc cggtgcgttg atggtgtcac gcacccagtc gatgatcatc
    10801 aggcggttat ccagttcctg gcgctgggca tcgtccagat ccggccactc cactttacgc
    10861 gtgcctttcg gggctttgta accttgccag aatgcccagc atcgatccgc atcccagcct
    10921 tcaccgctta actgaacatg cttgatgccc agaccgtcaa tcttgcgcgc cgcacgctgg
    10981 atcagcccca gatcgtctgc cccgttcaaa tgcagggtaa tgccgtcatt attaatgctg
    11041 taagttgctt tttctcccca gcgcgcgtcg gcaggttggg tagagagggt aatcttcatc
    11101 gcttctgtca ttttagttat ccttctttgt aaaagggccg ccagttggca gccccaggat
    11161 ttttccgcta aatgattcgc gttgcaggaa ggcggcaagt gagtgaagcc ccaggagcat
    11221 agataactat gtgactgggg tgaacgagcg cagccaacgc atctgcggcg tgaagcatga
    11281 cgcggaaatt attcggcctc gtccagccag actaacaaaa tcgcttcgag gattttctcg
    11341 ttggatgcct gcgggtcgtc gtcgaaatct tccagatcgc aaatccactg atgcatatcg
    11401 gtgaatcgaa ccgttttcgg atcaagatcg ggatacgcat cgtacagtgc ttcgccaatt
    11461 tcgcggctat cggtccactt aagtcccata ctaacctctg ttaatgctca cgcgcatggt
    11521 tgatcgtgta acgcgggatt tcgaccacta aatcttcgtc ggtgacgcgc gcctggcagc
    11581 ttaaacggct ttccggctcc agtccccagg ctttgtccag catgtcgtct tcctgctctg
    11641 agctttctgg aagcgagtca aaaccttcac gaacgatgca gtggcaggtg gtgcaagcac
    11701 aggatttttc acaggcgtgt tcaatctcga taccgttacg cagagctgcg tcgagaatgg
    11761 tttcaccgct attagcttcc agaacagcgc catcagggca gagatcctga tgaggcaaaa
    11821 taacaatctt tggcatatta aacctcgtcc acggaatggc ctttcagcgc acgacgaacc
    11881 gactggtcca tgcggcgagc ggcgaaatcc tgggtttgtt tgtctacgtt tttaatcgct
    11941 tgttcgatgg cgtcaacgtc atcgccctgc gccacttcac tcaggtgagc ggcagcatcg
    12001 tcaatgacct gacgttctgc ggcgcttaac agcgcggcat cagcagccag cgcgccgtgc
    12061 agactttcca gcacacgcgc cgcttctact ttttgttctg ccagcattcg ggcttttacg
    12121 tcctgctcgg catagctcat tgagtctttg atcatcgaag cgatttcgct atcggtcaga
    12181 ccgtaagacg gtttgacctg aatagacgcc tcaacgccgg tggatttctc catcgccgtc
    12241 acgctcaaaa gaccgtcggc atcgacctgg aacgtcacgc gaatatgcgc accgccagcc
    12301 ggtagcgccg gaataccacg cagcgcaaaa cgcgccagtg agcggcagtc ctgcaccagt
    12361 tcgcgctcac cctgcattac atggatagac atcgccgtct gaccatcttt aaaggtggtg
    12421 aaatcctgag cgcgggccac tggaatagtg gtattacgcg gaatcacttt ctccaccagg
    12481 ccgcccatcg tttcgaggcc cagcgacagt gggatcacat caagcaacag catttcgctg
    12541 tctggcttgt tacccaccag aatatccgcc tgaatcgccg cgccaatagc gacgacttta
    12601 tccgggtcga tggaagtcag cggtggacga ccgaaaaatt cgcctacccg ttcacgcacc
    12661 agcggcacgc gagtagaacc gcccaccatc accacttcca gcacttcatc agcttctaca
    12721 cccgcgtctt tcagcgcgcg acgacaagcc aataaggttc gttttaccag tggcgcgatc
    12781 agttcattga attgttcacg gctgatttcg ccctgccagc ccgcaacgtt aacggtcacg
    12841 gagtccgcat cgctcagcgc gattttggct gcaatggcgg catccagcag ttcacgctga
    12901 acgcggttat cgctacgatc aggaatgccc gcctgctcgc gaatgtaatc cgccagcaga
    12961 tggtcgaaat catcgccgcc gagcgcggaa tcaccgccgg ttgccagcac ttcaaacacg
    13021 ccgcgactta agcgcagaat ggaaatatca aacgtcccgc caccgaggtc ataaacggcg
    13081 atcacgcctt cctgaccgga atccagcccg taggcgatag ccgcagcggt cggttcgtta
    13141 agtaagcgca ggacgtgaag gcccgccaga cgcgccgcgt ctttggtgcc ctgacgctgg
    13201 gcatcgtcaa agtacgccgg aacggtgata actacaccat ccagctcgcc tgccagggct
    13261 tcagttgccc gcgccgccag tgctttgagg atgtccgcag aaacgcgcac cgggttcagc
    13321 agccccgccg ccgtttcaat catcggcagg ccgttttcgc tggcctggaa ttgataaggc
    13381 agatgcggat agcgttgctg gatatcagcc agcgagcgtc ccatcaggcg tttaacagaa
    13441 ctaattgtgt tggcggtatc gagcgctgca ttagtacgcg cgtcataacc caccgaatgc
    13501 ccttgctgtt gatagtgaac aacagatggc agcaggtgac ggccttcatg gtcggctaac
    13561 gtttcggcct gaccgctgcg cactgtcgcc accagcgagt tggttgtgcc caggtcaata
    13621 ccggccgcca gacgacgctg atgcggcgca gcactcaaac caggttcact aatttgtaat
    13681 aaggccatgt ttagcttcca gaaattaaaa atcgagcagt ttttcttcga gttgttcggc
    13741 actgcttcgc agtttatcga gaaaacgcag cttacgcacg gtatccgccg ccgcgtccca
    13801 ggtctcgttg tctaactgtt caaccatcaa ctgatggcgg gtatcaaaca tctttttcac
    13861 acgtttgata aagctttcca gccgcgcttc atctttcgcc tgttcgatct cgtccagctc
    13921 ttcgcgcagc tccaactgtt ccatcaggaa cgcggtgtcg cgcacagtat gctgctcgct
    13981 ggcgagatca aagccgtgca aagaaagcaa atattccgcg cgcattaacg gatgacgcag
    14041 cgtttgccag gcctgattaa tggttgcaga ttgctgtacg gcggcgagtt gttccgcctg
    14101 gcttccgctg gcgaatttat caggatgata ctgacgttgt agatcctgaa aacgcaggct
    14161 cagcgcctgg gtatcgagtt gataacgggc aggcaagcca aagagggtga agtaatccat
    14221 aacattctca gggctgcgat ccgccgcgat aacggcgaat caggtaaaac aacccccacg
    14281 cgcaggcgac cacggtgggg ttatcggtat gcgcatcaaa cgtggaagct ttcgccgcaa
    14341 ccacactcat ctttaacgtt cgggttggtg aatttaaacc cttcgttcag gccttctttt
    14401 acgaagtcca gctgcgtacc gtccagaaat tgcaggcttt tgccatcgac cacgactttc
    14461 acgcctttgt cttcaaacac gatgtcttcc ggcgtcggtt cgtcaacaaa ttccagtaca
    14521 taagccatac ctgaacatcc ggaggttctc acgcccagac gtaggccaaa ccctttgcca
    14581 cggttagcca gaaaggtatt tactcgcgct gctgcactgt cgctaagtgt aatcgacata
    14641 accaaacctc aactcttatt ttgcttcacg tttgctttta tagtccgcaa tggcggcttt
    14701 gatcgcgtct tctgccagaa tagaacagtg aattttcacc ggcggcagtt caagttcttc
    14761 agcaatatcg gtgtttttga tagcctgcgc ttcgtcgaga gacttccctt tcacccattc
    14821 ggtgaccagg gagctggaag cgatagcaga accgcagccg taagttttaa aacgcgcgtc
    14881 ttcaatgata ccttcatcgt tgactttaat ctgcaacttc attacgtcgc cacaggccgg
    14941 tgcacccacc atgccgctgc cgacgttctc gtcgttgttg tcaaaggaac ccacgttacg
    15001 cggattctcg taatggtcga taactttttc gctgtaagcc attataaatt ctcctgattc
    15061 cgataccgat taatgatgag cccattcgat gctgttcaga tccacgccct gcttgtacat
    15121 ttcccacagc ggagaaaggt cacgcagacg accgatggat ttacgaacta actcgatggt
    15181 gtagtcgatc tcttcttcag tagtaaaacg acctaaagag aaacggatag agctatgtgc
    15241 cagctcgtcg ttcagcccca gcgcgcgcag cacgtaggac ggttcgaggc ttgctgacgt
    15301 acaggcggaa ccggaagaaa ccgcgagatc tttcagcgcc ataatcagcg actcaccttc
    15361 aacgtagttg aagctgacgt tgagaatgtt cggcgcaccg tgttccaggt caccgttcag
    15421 gtaaacttct tcgatatctt tgatgccgtt ccacagacgg ttacgcaggc cgcgcagacg
    15481 ttccatctcg gtcgccatct cttcttttgc gatgcgatag gcttcgccca ttccgacgat
    15541 ctggtgaaca ggcagagtgc cggaacgcat accgcgctcg tgaccgccgc cgtgcatttg
    15601 cgcttcgatg cgtacgcgcg gtttacgacg cacatacagc gcaccgatac ctttcgggcc
    15661 atagattttg tgaccggaga aagacatcag gtcaactttc aactggctca ggtcgatagg
    15721 cagtttgccc acgctctggg ttgcatcaac gtgatagata atgccacgag cacggcacat
    15781 ttcgccgata gccgcgatat cctgcaccac gccgatttcg ttatttacgt gcatgataga
    15841 aacaaggatg gtgtcgtcac gcatcgctgc ttcgagttct ttcaggtcga taataccgtt
    15901 acgctgcggt gcgaggtagg tgacttcgaa accttcgcgc tccagctgac ggcaggtatc
    15961 caataccgct ttgtgttcgg ttttgctggt gattatgtgc ttgccttttt tctgataaaa
    16021 gttggctgca cctttgatcg ccaggttgtc agactcggtt gcaccagagg taaagacgat
    16081 ttcacgcgga tcagcgccga ccagatcggc aatctgatta cgggcgatat ctaccgcttc
    16141 ttcagcctgc cagccgaaac ggtgagaacg ggaggccggg ttaccaaagg ttccgtccat
    16201 cgtcataaac tgcatcattt tctcggcaac acgcgggtcc accggcgtgg ttgcggagta
    16261 gtcgagataa atcggtaatt tcattgctct ataaactccg tacatcactc aatgcaagga
    16321 accaggctac cggctggatg tacgaccgtg tttacggagc gcgatacgcc ccggcctgat
    16381 tctgaatact ttttattaag cgcgtaactt aacgtcgatc gcgtcttgtg tgcgggtgcg
    16441 tggcgcgtcg tgagtatgct gacgaccaga cacatccagc acttcctggt tgttaaccag
    16501 ttcgcctaaa gtaatgttat tgagaaaacc ggtaagacgg tcgctcaaat cacgccacag
    16561 cgcgtgggtc aggcatttat cgccgccctg gcagccgcct ttaccctgac aacgggtggc
    16621 atctacagat tcgtcaacgg cgctaatcac ttcgccaacg gcgatgctgc tggcatcttt
    16681 gcctaacaga taaccaccgc ctggtccacg tacgctggaa accagaccat ttttacgcag
    16741 acgagaaaac agttgttcca gataagaaag ggaaattccc tgacgttcgg aaatatcagc
    16801 caacggtacc gggcccgctt cagagttgag cgcaacgtca agcattgcgg tcacggcata
    16861 gcgcccttta gatgtcagtc tcatgtctta cttcacctca aactcgcccc tgcccggggt
    16921 tttttattgt aaaagtgggg gtattgcata gcagggtcaa gtctgacatt cccgagtaaa
    16981 ttggtcaact atttacttga ctgatttagt cgggtattta accttcagtg ccattttttt
    17041 atcgtggcgt ttgtacctgt tatccttcca gaccatgggc gggttgaggg caattattcg
    17101 cattagcgca ggcctgttcc cctcacccta accctctccc cgaaggggcg aggggaccgt
    17161 ccgtgcactt atctggctta gccctcaggc cttattacct ttattctgct gctctataga
    17221 agccagaatc ccgcgcagga tattcaattc ctggctttcc gggcgcgcac gggtaaacag
    17281 acggcgcaat ttattcatca cctgccccgg atggttttca cggataaaac cggttgccag
    17341 cagcgtttgt tccagatgac cataaaaacg ctccagatca tcgaccagcg gatacggcgt
    17401 ctcttcatgc tcgacctgct cgccattttc ctgagttgcc agccaggcca tacgcacttc
    17461 ataggcaata acctgaaccg ccatcgccag gttcagcgag ctgtattcag ggttagcggc
    17521 aatcgcgaca tgataatgac acttctgcaa ctcttcattg gtcaggccga cacgttcgcg
    17581 accaaacacc agcgccaccg gggtatttgc cgcttcagca acgcttttca gaccgcattc
    17641 gcgcgggtcg agcatcggcc acggcagcgt gcgggaacgt gcgctggtgc ccaccaccag
    17701 gctacaaccg gctaacgctt cgtcgagcgt atcgacgatg tgagcattac caatcacatc
    17761 gctggccccc gccgccaggg caatcgcctg ggagtcaggt ttcaccagtg gattaaccag
    17821 ccacagattg gttaatccca ttgttttcat ggcacgggca acagaaccca tattgccggt
    17881 gtgtgacgtc tccaccagca caattcgaat attttgcagc attgtctttc ttcgtctaaa
    17941 gattattcgc gcatcttatc ataaaacgaa gacagatgcc gatctcgctg ctatactctg
    18001 cgccgttttc ccgttcttta acatccagtg agagagaccg atgcatccga tgctgaacat
    18061 cgccgtgcgc gcagcgcgca aggcgggtaa tttgattgcc aaaaactatg aaaccccgga
    18121 cgctgtagaa gcgagccaga aaggcagtaa cgacttcgtg accaacgtag ataaagctgc
    18181 cgaagcggtg attatcgaca cgattcgtaa atcttaccca cagcacacca tcatcaccga
    18241 agaaagcggt gaacttgaag gtactgatca ggatgttcaa tgggttatcg atccactgga
    18301 tggcactacc aactttatca aacgtctgcc gcacttcgcg gtatctatcg ccgttcgtat
    18361 caaaggccgc accgaagttg ctgtggtata cgatcctatg cgtaacgaac tgttcaccgc
    18421 cactcgcggt cagggcgcac agctgaacgg ctaccgtctg cgcggcagca ccgctcgcga
    18481 tctcgacggt actattctgg cgaccggctt cccgttcaaa gcaaaacagt acgccactac
    18541 ctacatcaac atcgtcggca aactgttcaa cgaatgtgca gacttccgtc gtaccggttc
    18601 tgcggcgctg gatctggctt acgtcgctgc gggtcgtgtt gacggtttct ttgaaatcgg
    18661 tctgcgcccg tgggacttcg ctgcaggcga gctgcttgtt cgtgaagcgg gcggcatcgt
    18721 cagcgacttc accggtggtc ataactatat gctgaccggt aacatcgttg ctggtaaccc
    18781 gcgtgttgtt aaagccatgc tggcgaacat gcgtgacgag ttaagcgacg ctctgaagcg
    18841 ttaatgactc aggcgggtga tatcactcac ccgccctcgc ctttcaggcg ctattccgaa
    18901 atacttcctc accgctttac tttctttcgt cactctccca ccatctttcc ccgattaatg
    18961 gatgataaag aagtcgcaag gaaaggttat ggcactgcca gtgaacaaac gcgttcccaa
    19021 aattctgttt attctctttg ttgttgcctt ctgcgtttat ttagtgccgc gcgttgccat
    19081 caacttcttc tattatcctg acgataaaat ttacggtccc gacccctggt cggcggaatc
    19141 cgtcgaattt acggctaagg acggtactcg tctgcaaggc tggtttattc cttcttcgac
    19201 gggccctgct gacaacgcca tcgcaaccat cattcatgct cacggcaatg ccggaaatat
    19261 gtccgcccac tggccgctgg tcagttggtt acccgagcgt aatttcaacg tttttatgtt
    19321 tgattatcgc gggtttggta aatcaaaagg cacgccgtcc caggccggat tgctggacga
    19381 tacgcaaagc gccatcaatg tggtgcgcca tcgcagtgat gtaaacccac aacggctggt
    19441 gctgttcggg cagagtattg gcggggcgaa tattcttgct gttattggtc agggtgatcg
    19501 tgaaggcata cgtgcagtga tcctcgactc cacatttgcc tcttatgcaa ccatcgccaa
    19561 ccagatgatc cccggcagtg gctacttact tgatgagagt tacagcggcg aaaattatat
    19621 cgccagcgtc agcccgatcc cgcttttact cattcacggt aaagctgatc acgttatccc
    19681 atggcagcac agcgaaaagt tgtatagcct ggcaaaagag ccaaaacggt tgatcctaat
    19741 cccggatggc gaacacattg atgctttttc cgatcgtcac ggcgacgttt atcgcgaaca
    19801 gatggtgaac tttatcctta gcgcgttgaa tccgcagaac taacccatga tcgctagcac
    19861 gataatcatt cacaaaatca ccttaagaca tgctaatcca ctggtcagga cagtttaaga
    19921 tgagaaaaat tctgtgacgc ttgccaacat ttctgatgat tagcattccc ttcgccattt
    19981 ccctgagcaa actttagcta ttcttatcaa ttatgcttat gggagatcta cagatgatgc
    20041 ctacgcttgc tccaccatct gtcctttcgg ctccccagcg ccgctgccag atcttgctga
    20101 cgctctttca gccggggttg accgccacca tggcaacctt cagcgagctt aatggtgtgg
    20161 atgatgatat tgccagtctt gatatcagcg aaacaggacg ggagatcctg cgctatcatc
    20221 aactcacact gacgactggt tatgacggta gctaccgggt tgaaggtaca gtgcttaacc
    20281 aacgtttgtg tttatttcac tggctacgac gtggtttccg tctgtgtccg tcatttatta
    20341 ccagccaatt cacccccgcc ctgaagagtg aactgaagcg gcgcggaatt gcgcgtaact
    20401 tttacgacga taccaatcta caagcgttag tgaatctctg ctcccgacgg ctgcaaaaac
    20461 gctttgaatc gcgcgatatt catttcctgt gtctgtatct gcaatattgt ttgctgcagc
    20521 accacgctgg aattacgcct cagtttaatc cgctccaacg tcgctgggcg gaatcctgcc
    20581 ttgaatttca ggtagcgcag gaaattggac gccactggca gcgtcgggcg ctccagcctg
    20641 taccacctga tgagccactg tttatggcac tacttttttc catgttgcgg gttcccgatc
    20701 cattgcggga tgcgcatcag cgggacagac aattgcgtca gtctatcaaa cgtctggtaa
    20761 accattttcg tgagctggga aatgttcgtt tttatgatga acaggggtta tgtgatcagc
    20821 tttataccca cctcgcccag gcgttaaatc gcagtttgtt tgccatcggt attgataata
    20881 ccctgccgga agagttcgcc agactgtatc cacgcctggt acgcaccacc cgcgcggcgc
    20941 tggccggatt tgaaagtgaa tacggcgtcc atctttctga tgaggaaagt ggtctggtcg
    21001 cggtgatttt cggtgcctgg ctaatgcagg aaaacgatct gcatgaaaaa cagattattc
    21061 tactgaccgg gaatgatagc gagcgagaag cgcagattga gcaacagtta cgcgaactaa
    21121 cgttactgcc gctcaacatt aaacatatgt cggtaaaggc atttttgcag acaggcgctc
    21181 cgcgtggcgc ggcactgatt attgctcctt ataccatgcc gttaccgctc ttttcaccac
    21241 cgctgatcta tacggacctg acactgacaa cacatcaaca ggagcagatc cgcaaaatgc
    21301 tcgaatcagc atgagggaac aactttcggg cgcaaaaaca ttgccggaag cgccaccagc
    21361 gccatcaccc agaacacgcc gtggcccaga tattgataca ggaaaccggc gaaaacggtc
    21421 atgatagcga tactgccgcc catcgcgacg gcagagtaaa ccgcctgtaa acggatgact
    21481 tcgctaccct ggcgagcagc aatatagcgc atggcggcca ggtggcagac cgtgaaggtg
    21541 ccgcaatgca gaatttgcac cactatcaac cacggcaacg ccgtagttgc tcccataatg
    21601 ccccagcgca ctacgccgca aatcgctgag atcaacagca tatcgcgtgc actacaacgg
    21661 cggaaaagtt tattactcag tgcaaagata atgacttccg ccaccacgcc cagcgaccac
    21721 aaatacccca ccgccgaggc cgagtagcca gctgcctgcc agtaaatggc gctaaaaccg
    21781 taataggccg catgtgcccc ctgcaataaa caaacgcagg ccagaaagcg ccagttctgg
    21841 cgaaccagcg ccaaccacgc tgaccaaccg gtgctctcct gctggcggct tgccccttgt
    21901 ggctgaatcg tcggacggat gagaaagccg agcagcatgg atgccacgcc caacgtcaac
    21961 agcacgagga tcacccgata atcaaacata gtgaccagtt tgcccgtcag cgccgagcca
    22021 atgacaaacg ccaccgagcc ccacagtcgc actttgccgt aatcaagcgg gaactgcttt
    22081 tgccacgtat tcgccagtgc atcggtcaac ggtaccagcg gtgagaaaaa gaggttaaag
    22141 ccaatcatca ccagcatcag ccacgctacg tgcgcccccg cccagaaggc gacagcaaag
    22201 agaagtgtca gcagtgccag cacgcgcaag gcggaaatca ggcgagaagg atcgctgacg
    22261 cggggcgcga tgagcaaact cccgagaaaa cgggcaacca gacctgcccc caataacagg
    22321 ccgatggttt ctggcgttaa accaatccct ttaagccaga cgctccagaa aggtagaaaa
    22381 atgccgtaac taaaaaagta tgtgaaatag ccgagcgcca accagcgcgt ggattgcaaa
    22441 accatgagtc cctcccgtca ggaggcgtta gtctggagtt aatctaatgt tggtgcaagt
    22501 tgaaaaataa gaatattcga gcctgcttcc cgatgtctgt aacgccagtt cacaagcact
    22561 caaaaactgc gatccgtgac gcatgttgca aagataatgc gtaattatgc cgttacgctt
    22621 gccaaacgtt cctgcacaat ggcgatgaaa tcgcgtaatg ccggcttcat ctcccctttc
    22681 ttccacgcca tcagtaaagc aatggaaggc acatttccgg caatgggacg aaaaacaacc
    22741 tgcccggtat taaaattatt catataaccg ggtatcaaag tgacgcccag ccccatgccc
    22801 accagattca tggtcaccag aatattcgtt gccacctgga cgatatttgg ctggctgttt
    22861 tcttgtgcaa accacgcttt aacgatcggc gcaagcgaac cggaatacgc cggatcggta
    22921 ctgacgaaat tcacgccatc cagttgcgcg gcggtgatct ctttttcatg tgctaaaggg
    22981 tgatcaaccg gtaacaccac cactaatggt tcgtcaaaaa gctccagata atcaatctcc
    23041 gggctataaa caggatggcg cattaagccg acgtcgagtt caccacggcg aattttttcc
    23101 tcctgttgcg tggtgattaa actcaccagc tcaatcaagg tgtctggctg tctgagacga
    23161 aacatcggta atacttttgg cagtaaattc acttccgccg atggcacaaa gccaatggtt
    23221 aattgtctgt cttcctgaac aattttccgc gcccgtaatt tggcattttc cgcttgttcg
    23281 aggattgcca gcgcgtcctg gagaaaacat tctcccgccg ccgtcaacgc gactttgcgc
    23341 ttatccctca ccagtaacgg aacaccgaca cagttttcaa gatcgcggat ctggctgctt
    23401 aacgaaggct gtgaggtatg cagtttttcc gccgcacggg taaagttcag tgcctgcgcc
    23461 actgcgacga aatagcgtaa atgccgtagt tccatcacct tccccttgtt atcgaaaaaa
    23521 cgtctcagct ggtagaaatt aactatttca catattagca accaaccagc aacatcctta
    23581 tgacacaaaa atagaaggtc aatacatctt atctttcagg attaaaaaat atgaccacac
    23641 cctcagattt gaacatttac caactgattg acacccaaaa cggtcgggtc actccgcgta
    23701 tttataccga cccggacatt taccaactgg agcttgagcg tattttcggt cgttgctggc
    23761 tatttctcgc ccacgaaagc cagatcccaa aacccggtga tttctttaac acctacatgg
    23821 gagaagatgc agttgtcgtg gtgcgccaga aagacggcag catcaaggcg tttctcaacc
    23881 aatgccgcca ccgggccatg cgtgtgagtt atgcagattg cggcaacact cgcgccttta
    23941 cctgcccgta tcacggctgg tcttatggca ttaacggcga gttgatcgat gtaccgctgg
    24001 aacctcgcgc ctacccacaa gggttgtgta aatcccactg ggggctaaac gaagttcctt
    24061 gtgtggagag ttataaaggg ctgatttttg gcaactggga taccagcgca ccgggcctgc
    24121 gtgattacct gggtgacatt gcctggtatc tggatggcat gctggatcgt cgcgaaggcg
    24181 gcaccgaaat tgtcggcggc gtacaaaagt gggtgatcaa ctgcaactgg aaattcccgg
    24241 cagagcagtt cgccagtgac cagtatcatg ctctgttcag ccatgcttct gccgttcagg
    24301 tattaggggc gaaagatgat ggcagcgata agcgcctcgg tgatggacaa accgcccgcc
    24361 cggtgtggga aaccgccaaa gatgccctgc aatttggtca ggatggtcac ggcagcggct
    24421 tcttctttac tgaaaaacca gatgctaatg tctgggtcga tggcgcagtt tccagctatt
    24481 accgcgaaac ctatgccgaa gctgaacaac gtttaggtga agttcgcgcc ctgcgcctgg
    24541 cgggtcataa caatattttc ccgacacttt cctggctcaa cggcactgcc acactccgcg
    24601 tctggcatcc gcgcggccct gatcaagttg aagtgtgggc gttctgtatt actgacaaag
    24661 ccgcctccga tgaagttaaa gccgcttttg aaaacagcgc cactcgtgct tttggtcctg
    24721 ctggttttct cgagcaggat gactcggaga actggtgtga aatccagaaa ttgcttaaag
    24781 gccaccgcgc ccgcaacagc aaactgtgtc tggaaatggg gcttggtcag gaaaagcgcc
    24841 gcgacgacgg cattcctggc attactaact atatcttttc agaaactgct gctcgtggaa
    24901 tgtaccaacg ctgggccgat cttctgagta gcgaaagctg gcaggaagtg ctcgataaaa
    24961 ccgccgctta ccagcaggag gtgatgaaat gagtgcgcaa gtttcactag agttacatca
    25021 ccgcattagc cagtttctct ttcacgaagc cagcttactg gacgactgga aatttcgtga
    25081 ctggctggca cagctcgacg aagagatttg ttacaccatg cgcaccacgg tgaacgcgca
    25141 aacgcgcgac cgccgcaaag gtgttcagcc gccgacaacc tggattttta atgacaccaa
    25201 agaccagctg gagcggcgaa tcgcccgtct ggaaacgggc atggcatggg cagaagagcc
    25261 gccgtcacgc acccgccact taatcagcaa ctgccagata agcgaaaccg acatcccaaa
    25321 cgtatttgct gttcgggtga attatctgct ttatcgggca caaaaagagc gcgatgaaac
    25381 gttctatgtc gggactcgtt ttgacaaagt tcgccgtctg gaagatgaca actggcgctt
    25441 gctggaacgg gatatcgtcc tggatcaagc ggtaatcact tcccataacc tgagtgtact
    25501 gttctgatga atcgaattta tgcgtgtccc gttgcggatg tgccggaggg tgaggctctc
    25561 cggatcgata cctcgcccgt catcgccttg ttcaacgttg gcggcgagtt ttatgccatt
    25621 aacgatcgtt gcagccatgg taatgcgtcg atgtcagaag ggtatctgga agatgacgcc
    25681 acggtggagt gcccgctaca cgccgccagt ttttgcctga aaacggggaa agcgttatgc
    25741 ctgcccgcca ccgatccgct caccacttat ccagtacacg ttgaaggtgg tgacattttc
    25801 atcgacttac cggaggcgca gccatgagcg atctgcataa cgagtccatt tttattaccg
    25861 gcggcggatc gggattaggg ctggcgctgg tcgagcgatt tatcgaagaa ggcgcgcagg
    25921 ttgccacgct ggaactgtcg gcggcgaaag tcgccagtct gcgtcagcga tttggtgaac
    25981 atattctggc ggtggaaggc aacgtgacct gttatgccga ttatcaacgc gcggtcgatc
    26041 agatcctgac tcgttccggc aagctggatt gttttatcgg caatgcgggc atctgggatc
    26101 acaatgcctc actggttaat actcccgcag agacgctcga aaccggcttc cacgagctgt
    26161 ttaacgtcaa cgtactcggt tacctgctgg gtgcaaaagc ctgcgctccg gcgttaatcg
    26221 ccggtgaagg cagcatgatt ttcacactat caaatgccgc ctggtatccc ggcggcggtg
    26281 gcccgctgta caccgccagt aaacatgccg caaccgggct tattcgccaa ctggcttatg
    26341 aactggctcc gaaagtacgg gtgaatggcg tcggcccttg tggtatggcc agcgacctgc
    26401 gcggcccaca ggcgctcggg caaagtgaaa cctcaataat gcagtctctg acgccggaga
    26461 aaattgccgc cattttaccg ctgcaatttt tcccgcaacc ggctgatttt acgggtccgt
    26521 atgtgatgtt ggcatcgcgg cgcaataatc gcgcattaag cggtgtgatg atcaacgctg
    26581 atgcgggttt agcaattcgc ggcattcgcc acgtagcggc tgggttggat ctttaaggaa
    26641 gtacgatgaa agaaaaaacg atcattattg tcgggggcgg gcaagcggcg gcaatggctg
    26701 cggcctcgct acgccagcaa gggttcaccg gtgagctgca tctgttttcc gatgagcaac
    26761 atcttcctta tgaacgccct ccgctctcga aatccatgtt gctggaagat tccccacagt
    26821 tgcagtctgt gttacccgct cactggtggc aggaaaacaa tgttcatctg cattccggtg
    26881 taaccatcaa aacattgggc cgcgacacac gagagttagt gttagccaac ggcgaaagct
    26941 ggcactggga tcagcttttt atagcaaccg gcgcggcagc tcgaccgctg ccgttgcttg
    27001 atgcactggg agaacgctgc tttaccctgc gccatgccgg tgatgccgcc agactgcgag
    27061 aagttctgca gcccgaacgg tcagtcgtga ttgtcggtgc cggaactatt ggtctggaac
    27121 tggctgccag cgccacgcag cgcagatgta aggtgacagt gattgaactg gcggcaaccg
    27181 tcatgggccg taatgcacca ccgcccgtgc aacgctatct tttacagcgc catcagcagg
    27241 ctggtgtgcg cattctgctc aataatgcca ttgaacatgt ggtcgatggt gaaaaagtag
    27301 aactgacgct gcaaagtggc gagacgcttc aggccgatgt ggtgatttac ggtattggta
    27361 tcagcgccaa cgaccaactg gctcgcgagg ccaaccttga tactaccaat ggcattgtca
    27421 ttgatgaggc ttgccgcacc tgcgatcccg cgatctttgc cggtggcgat gtggcaatca
    27481 ctcgtcttga taatggtgca ctacaccgct gcgaaagctg ggaaaacgcc aataaccatg
    27541 cgcaaattgc cgctgccgca atgttagggc taccgcttcc gctactgccg ccgccgtggt
    27601 tctggagtga tcagtacagt gataacttac agtttattgg cgatatgcgt ggcgatgact
    27661 ggctttgtcg tggcaacccg gaaacccaga aagcgatttg gtttaatctg caaaacggcg
    27721 tgcttatcgg tgcggtaacg ctgaatcagg gacgtgagat tcgctcaatc cgcaaatgga
    27781 tccagagcgg caaaacgttt gatgcgaaac agctgacaga tgagaacatc gcgcttaaat
    27841 cactgtaacc aggataatta gcgaatatct caacgcctgg ggcgtggcga ggtaccagag
    27901 tgtgtattac gtttaaatca cattatcttg caaagggatt ggttatgaac tcactacgtt
    27961 attttgattt tggtgctgcc cgccccgttt tgttattaat tgcccgtatc gccgtggtcc
    28021 taattttcat tatttttggt tttcccaaaa tgatgggctt tgacggtacg gtccaatata
    28081 tggcctcgct gggcgcaccg atgccgatgc tggcagcgat tattgcggta gttatggaag
    28141 tgcccgccgc gatattaatc gtgcttggct ttttcacccg tccgctggcg gtactgttta
    28201 ttttctacac gctgggtacg gcggtgattg gtcaccatta ctgggatatg accggcgatg
    28261 ccgtcgggcc aaatatgatt aatttctgga agaatgtcag tatcgctggc gcgttcttgc
    28321 tattggcaat taccgggccg ggggcaattt ctctcgatcg ccgttaggaa agatgccgga
    28381 tgcggcgtga acgccttatc cggcatttta taaattacag ccactctacc cgcaacgaca
    28441 tctctgaggt tgttgattcc cccggcgcaa gcgcaatgag gtcaccccct tccgggcgat
    28501 gatggtcatc cggcgcatgg ctcatcggct ccagacagaa gaaatcaaac gcatatcctt
    28561 tatcaaacgc cgggtcggaa acaaagatga aataacacgg tgcaggtggc gtcgtttcta
    28621 tgatgatggc ataaccctcc tgcggctgct cgatgcgggc ctgaccattc catccggcaa
    28681 aaccattgtt cacccactgg cgcggcaacg gcgctagctg gttaaaatcc agttcttgcg
    28741 gtagctgctc gcaaaactca cccgccagcc actgctcccg ctccagccag taaccgctcg
    28801 cctgtgcctg aatccgcgtt tgcggcgaca acggaaaata aggatgccag ccggtaccaa
    28861 atggcagcgt ctctgcccct tgattggtga cagagagcgt caccgtcagc gtatccgccg
    28921 ttaaatgaaa cgcctgactt acccgatagt gatagacacc gctgcgatgt tcatacacca
    28981 gacacaaact atcatcgctg tgcgagacac attgccactc gcccagccag ccatcgccgt
    29041 gtagatagtg cgcatcccac tcaacattcg gttgcagttg atactcacgc ccctgccaga
    29101 caaaacggtt gccgctcacc cggtttgcaa atggcaccag cggaaaacaa gaggcatcgg
    29161 ttgccacacc gcttttttta ccaggacgta agagcggcgt cgtatcgcgc cagaagcctt
    29221 cgattacgcc gccctggtca gaaacgccca gctttagcga cccgtgggat aaggtataga
    29281 tggtcatccg ctaactcctt aatccgggaa gttaatcaca actttcccgc atttgccgct
    29341 cgccatcagc gcataggcat cacctgcctg ttccagcgag aagcgatggg taatggcgtt
    29401 acgcggccac agcttccagt cagtcaaatc gtgggcgcat ttttccatat ggaacagact
    29461 ggtcacccag gagccaataa tccgccgttg atggtgcatc agatcggcgc tgacctcgaa
    29521 ttccactttt ccggtttcac caatgtaaac cacccgcccc cagtcagcgg tggattgcag
    29581 tgccagtaag cgacctgcgg cattaccgga acaatcaagc gcaacatccg cgccgccgtg
    29641 agtgagttcg gcgattatct gcggcagacc ttcagtggtc gctaaatagc cgtgatccat
    29701 cactcctaac tgttttgcca tcgccagacg ttccggcagc atatcaacgc cgataatccg
    29761 ttttgcaccg cgacctttcg ccagcatcat ccccatcatg ccgactggcc ccagaccgac
    29821 caccagcacg ttatcactgc cggaaacttc gccgcgcaaa atcccttcat aggccgtgcc
    29881 aacgccgcaa ctgataaacg caccatcttc gtagctcagc gcatccggca ggaggatcag
    29941 atctttttct tccgccagca agtattcggc atgaccgccg tcacgctgcc agccgtaagc
    30001 cgcttttcct tcgccagtac aagaaatagg aaaaccgcga cggcagttcg ggcaaaaacc
    30061 acagccagaa atgtgataca ccagcacgcg gtcgccctct ttaaaatggc ggcagccttg
    30121 ccccatcgcc acaatctgcc cgcacggttc atgaccgttg ataaatccct agtacaacgg
    30181 tttatcggat gccgccgccg tagcgcggtg ctggtggtag atatagtgga catcgcttcc
    30241 gcaaattccg gaggatttca ttttgatcag tacctggtta atccccggcg tcggcactgc
    30301 aacttcccgc agatcgacgg tcgaatttcc tggtaaataa gctgccagca tcgttttcat
    30361 aaatccctca ttaacaatac gattaatttt catccctgcc gcacccgcgc caggggcgtt
    30421 aaggtttagc gtttcgcttt actgctacgc tgggtcagca agatattcgc cagcaccgcc
    30481 accacgatga tgacgccgcg caccacctgc tggaaaaagg agttaatccc gagcagcacc
    30541 agaccgttac cgattagcgt aatcaccagt acgccaagca gcgtaccgaa caacgagccg
    30601 cgaccaccgg aaagcgccgt accgccgacc acgaccgcgg cgatgacgtc aaactccaga
    30661 ccgtttgcgg cacctgcgtt accggaaccg aggcgcgccg ccaacaaaat gccggtcacc
    30721 gccgctaata atcccgaaag ggtaaagata agaatgcgta cccgacgaac gttaataccg
    30781 cacaactgcg ccgccgtggc attaccgcca acggcaaaaa ccgagcgccc gaaggcggtt
    30841 ttgcggctaa tgaacacaaa cagcgcaaac agcaccatca tgatcagcgc ggataccggc
    30901 acaccgagaa attgtccgcc cagccagtcc agcacctcgt tttcgtcaat cggcactggc
    30961 agagcgttgg tcataaacaa gcccattccg cgcagggcgc tccacagccc cagcgtggcc
    31021 acgaaacttg gcacgttaaa cacgccacgc agcaccccgg cgagcgttcc catcagcgcg
    31081 cctaacagca acaccagcag acaagcaatc gccaacggaa cttcaaattg cagcaaaaat
    31141 gccaggcata ccgagacaaa agccaccatc ggcccaacgc tgacatcaat ttcaccggag
    31201 ataataatca gcgtcatcgc ccaggcggca atcccaatgg tggcggcatc gcgcagcacg
    31261 ttcatctggt tattcaatga gataaagcca ggcgcgttca gggagaagac cagataaaga
    31321 atggcaatca ccaccagcaa accgatctca ttaatatggt gactgacaaa ttgtttgagc
    31381 gagacgctct tgccctgcgg caatggtaat gacgaagcag acatcttcag gttcctcggt
    31441 aaaattcatc agtgcacaga cagaatggcg gacatcagct catccacatt gaccggagcg
    31501 tgaaactcct gcgagaacgt gccgtgctgt aataacagga tgcggtcaca caccaacggc
    31561 aactcctcca cttcactgga gataaacacc acgctttttc cttcggcagc cagctcacgg
    31621 acaatacggt aaatctgctg tttggcttcg atatcgacgc cgcgcgttgg ctcgtcgagc
    31681 aacaaaatct ggctggcggc atagacccaa cgaccgatca ctactttttg ctgattgccg
    31741 ccagaaagcg tgccgatggg cgtttcgcta ctggcggcct tgaccgtcat ccgctgcatc
    31801 acttcttcgg tcaggcggcg gatggtggac cattgcagca ctccgttggc gctgattttt
    31861 tgccgattgg tcagcactgt attttcgtca acgcccaacc agggaatgat ccccgcttct
    31921 ttgcggtttt ctggcgtata gccaatgccg cgtttcagca tgtcgccgta atcggggcgc
    31981 gtgattttct cgccgttgat aacaatttcg ccctgttcat acgtctccag cccaacaatc
    32041 gccttcagca attcactgcg ccctgccccc agcagaccag caatgccgag cacttcgcca
    32101 cgacgtagcg taaagctgat atcctccagc ttgggcttat ggcgtaacgc acggacttcc
    32161 agcacggcct gatccacaat ttcctgaggt gcaaccggcg caatatcaac gtgatcgcgc
    32221 cctagcatta gcgacacaat atgatgcgtg gaagtgtttt cgagcatcac atcgccagcc
    32281 acctgcccgt cgcgcataac ggtggcactg gaggcaatgc ggcgaatttc ttccatccgg
    32341 tggctgacat aaatcaccgc cacgcccagt gctgacatct ttttcaccgc tctgatcacc
    32401 agttcaactt ccgcactcgc aagcgaactg gtaggttcat caagaatgac cacgcgcggc
    32461 tcgcccttca tcacccgcgc aatttccacc agctgcttat gcgccgggct tagtgttgaa
    32521 acaagttgtt caggactaac gtcaacgccc agcgcctgta agcaacgttg ggcatcctgc
    32581 gccatttgca ggtaatcaat catgccgttg cggcggggcc actgaccgag gcagaggttt
    32641 tccgccaccg tcagcccttc caccagactt aactcctgat aaaccgcgcg tacccccagt
    32701 tcagcggcac ggcgagtcag cgtagcttcg tcaccttcca gtcgcgtctc gccaatccag
    32761 atttcaccgc tatccggacg ttcactgccg gtaagcattc gaatgagggt cgatttgccc
    32821 gcgccgtttt tgcctaacag cgcacgaacc tcgcctttat tgagcgtgaa gttaacgtta
    32881 tccaacgcaa cgacgccggg ataacgctta tttcctgcca ccacttttgc taccgggact
    32941 gcctctgttg ccgtgaacat agggacctct gcgaatcagc gattagggca gaccatcaac
    33001 gtgcgttgcc agccactgtt taccgtcttc cgttttggta tagagatcga taggcacctg
    33061 aatcactttt tcaccgtcgg cttgtttatt gataaccttc aatgtttgcg cgaaaacagc
    33121 attgcccatt ttcttaccgg aaatatccac taccgctttc agcacctgat tgttttccag
    33181 ctcctgagca atttcggttg tcatatccga accgaaaaca gcaatttttc cggcctgatt
    33241 ttgattacgt accgctttta ccgcgccgag tgtcgcaccg cccgattccc ccataatggc
    33301 gttgagatcc ggcgtggaga taatcagttt ttcaccaacg gaaatcgctt tatctaaaac
    33361 agtcccttcc tgattagcga caatttgcgc gccgggaacg cgggatttta atacttcttc
    33421 aaatccttta cgtcgctgca cacaaacttc aaaggcttcg caattgatga cggcaatttt
    33481 cggctggtca attttattgg caataaaata atcggcggca gcgttaccca gttttttacc
    33541 aaattccagc gggtcaccca ccagatacgc cgagacatat ttatcgaccc ccttttgatt
    33601 aatacaggtg ttgtagcaaa tcaccggaat acccgcttca ctggcgcgac gaacggtacg
    33661 gctactgcca ttttcagaca ctgccgataa aataatagca tcgacattac gcgccacgag
    33721 ggtatcaaca aaggtacttt ctttcgaaat atcaccctgg gcgttagttt caattaactg
    33781 tacctgtact gaagaatctt ttgccgcatc ctgaactccc tgacgcactc cagcgtaata
    33841 tccctgagta tcaaggtata ttgcgccaat ggtcatttct ttatccgcag ctctggcaaa
    33901 tagtgcgcta cctaatagcg tagccattaa taatagatta cgggttgttc tcattttttt
    33961 aggcatagag cctcctgtag ggtttttatt aacaacggct tattctaatt attttgtgat
    34021 gagcggcagc gcgtgccgct caaatattac agttaatagg gtttagtgaa cgtagttaaa
    34081 aataaaaggc atcacggtgg tgaataatgc cgctttcggc caggccggat taatcgccgt
    34141 caattcagcg cgagcagatt cgtattgcgc aatctcgcct aatcccgcgg ccgccagcat
    34201 cgccaccatc aggcattttt ctttatgctg ctgttgtaaa tcgccataca gcgacaacaa
    34261 gtcaggctgc gagacggcaa agaaatccgc ttcgatactg gttttcgcca tctcttgcgc
    34321 ccactgtttc atttcactaa acagttgctg tgcggtgtgt tgttcgccca gtaatcgcag
    34381 cgccattcct tgccagaaga ggtaatcaac cggctgatcg ttgtaatagc tgtggatgtt
    34441 aatggtgcga tcgccggtcg ccgccagatg taaacagcgc gtcgcttcag tttcatcgcc
    34501 ctgggctttg gcgcatatcg cctgccagaa ccagatgtcg ttatcagttt gccccggtaa
    34561 acggccttcg cttaaattct ccggataatg cagcgcggca tgaagcagtt cgctggcctg
    34621 ctgcggctct ctggcatcaa gatgctgcca ggcgcgtaat aactggttga ggataaactg
    34681 actggtgacc ttcccttccc cgccttccca cgggtggaat ttgcgcgtgg cgagaatgtc
    34741 cgccgctttg tctgcctgcc ccgtgagatg ccacaaattg agcagttctg cggtcatgtc
    34801 gtcgcgtttc agcgcaattt ccagattatt ttccagacgc gccagtcgtt tctccggtgt
    34861 ggttccactt agcttatcaa gcaaatcccg ttcgaaaagc agacgtgcat cctgcggcgc
    34921 aagctgataa gcattatcaa gataacgcgc ggccagctca taatcgtgtt gcttattcca
    34981 cgcatggatc gctaacccgc gccagccgtc ggcaaactcc ggcgacatct ctacgcaacg
    35041 ttgccataag gcaatggctt cgttgtagct acgtttgttg tagtagaagc aggccagtaa
    35101 atggcgagca aaccagcact cttcaatact ctccagcgcc gccacttctt ccagcgtatt
    35161 cgggaaacgg acaaactgcg ggaagacatc aatggctttt gcgaccagtt cgccacgttc
    35221 ggctttcggc agcaagctgg cttgcaggta aagcggcagc gtgcgctggc agtccagtgc
    35281 attcagcatc tctgccgcga gggtgggcat tccccagtta atcagttgcc cggcggtcat
    35341 cagggcgtta acgtcgcgtc cctgacacag accgcgccac tgcgcgagag ctgattcgct
    35401 acgaccatcg aaccagttca ggcaccacag agtggcgttc agcggataat cgcgcagcag
    35461 tttctcgcgc tgcaaacgcg cgttgtcctg acgaccactt aacaccagca gcagattatg
    35521 caggcaaagc acttcctgat tgattgggca ggcgcgaaga ctttgttggc aaaaatccag
    35581 acccgcgtcg aagttaccat tacgcgccgc cagtcgtgcc agaccataat agcctccggc
    35641 tttactgttg ccgctccaga ccgcacgcca gaaatcctct tcggcttgtt gatattgtcc
    35701 ctgacgttcg taagcactgg cgcgaatcaa actcgcctgt ccgcactgcg gatttttgtt
    35761 cagcgcatgt gcgcgtttca gagcctgagt ggcatacgcc accgcttgcg ggaaatctgc
    35821 gcggttatac tccagcatcg ccagcgccag attacagcgg taatccagcg gatccagcgc
    35881 cacgccgcgc aggtagtaat cgaacggtga acggctggcg tgatgatatt gctccagatg
    35941 ctgaccgata aaccaggctt catctgtact ggtaatgtct tgtgctgaca gtggcgcttt
    36001 ggcgacgtcc ggcaacggca actcttgcgg ctgatgttcc tgataactca gtacaatatt
    36061 gccgtcggca tcagagagct caatcgtcag tctttcagga ttgataccgt gtaacacgcc
    36121 ctggatggcg gtcgctggtg tcagggccac ggcatcatcg agcaacgcgt cgcatttgcc
    36181 gatttcgcgg atcgccaggc ggtatccgtt caacggagag atggcataca gcccccactc
    36241 aatcccccgc tcactacgct ggagtttgat taccgcatcg cgggaggcgt tttgcaccat
    36301 gcccagagaa tggtaaggca ggaaaaactg ctcaaaacgc ttctcttcgt aagcatcaag
    36361 ccgggtaaaa tcaggctggt tatcggcaaa aataccggtc atcagttcga tatacgggcc
    36421 gttattatca gttaggctct tatcccacgc ctggccaaat tcactgtgtc cccagctcca
    36481 ctgcttttta cctggcgcaa tatggtggtt ggcaacgtgt agcaaaccgc catcttcatc
    36541 gtgacaccac gcgccaacaa aatcgtactg tgatttttca gccatatatg aggttggaac
    36601 gggcacattt ttatagcgag aaatgtccac tccagcggag tagtccactt tgtagtaagt
    36661 gccggtggcg atagggaaag cggagacggc ccgtttgccg tgatcaaaca ccgccgttac
    36721 atccggcgga aagacgctct gatgcccttc accccctttc actgccgggt tggcccacca
    36781 caagaaatga cgcggcgtgg cgttgccgtt atagacgcgg ctggcgattt ccagcgccgc
    36841 ccggtcaggg cgcagggtga aacctgtcat cacctgtaaa ccatgcatcg gctccgtttc
    36901 gcctacccac accgtctgtg cgccgtcttc atgggcttcg agggtgaaat caacgggcat
    36961 aaaggtggtc gggcgatggt gttgcggcca gttaaactca atcccgccgg agatccacgg
    37021 tcccagcagc cccaccagcg caggtttaat gacttcattg tgatagacaa aatcgcgctg
    37081 tttcacttta tcccatgcgc gatgcacccg accgcccaat tccggcagga tcatcacttt
    37141 gatgtagtcg ttttccagcc acaccgcctg ccaggatttc agggttttct gctcgctcag
    37201 cgtatcggtc acgccgtagg gataaaccgc gccggacgat ccctgataaa cgcgattttc
    37261 caggaacatg ggatgtatat cctgcggccc ggtttcatag gtcgggatct caacgcgctc
    37321 ttgccacact tttactggag tcatggtgcc ctcaatatta acaagacata ctgaattaaa
    37381 agatttgtgg cagtgtattg aacaatctgg caatgttttc gcggaataat cacgcaatta
    37441 actaaacaag gtttagtgaa gatgagagcc tgcattaaca atcaacagat tcgccaccat
    37501 aacaaatgcg tgattctgga actgctgtac cggcaaaagc gcgccaataa atcaacgctg
    37561 gcccggctgg cgcaaatttc gattccggca gtcagtaata ttttgcagga actggaaagc
    37621 gaaaaacggg tggtgaatat cgacgatgaa agccagacgc gcgggcatag tagcggtaca
    37681 tggctgattg cgccggaagg tgactggacg ctgtgtctga acgtgacgcc caccagtatt
    37741 gagtgtcagg ttgctaatgc ttgtttaagt ccgaaaggtg aatttgagta tttacagatt
    37801 gatgcaccga cgccgcaggc gctgctgtcc gaaatcgaaa aatgctggca tcgccaccgt
    37861 aaattgtggc cggaccgcac catcaatctg gcgctggcaa tccacggtca ggttgatcca
    37921 gtgactggag tgtcgcaaac catgccgcaa gcgccgtgga caacgccggt tgaggtgaag
    37981 tatctgctgg aagagaagct gggcattcgg gtgatggtcg ataatgactg cgtgatgctg
    38041 gcgctggcgg aaaaatggca aaataattcg caggaacggg atttctgcgt gatcaacgtt
    38101 gattacggca ttggctcgtc gttcgtgatt aacgagcaaa tttatcgcgg cagtttgtat
    38161 ggtagcggac agattggtca caccatcgtt aatccggatg gcgtcgtctg cgactgtgga
    38221 cgttacggct gcctggaaac cgtcgcctcg ttaagtgcat taaaaaaaca ggcgcgggta
    38281 tggctaaaat cacaaccggt taatactcag cttgatcctg aaaaactgac tacagcgcag
    38341 ttaatcgctg cctggcaaag tggagaaccg tggatcacca gctgggttga tcgctctgcc
    38401 aatgccattg gtttgagtct gtataacttc ctcaacatcc tcaatattaa tcagatttgg
    38461 ttgtacggtc gcagttgtgc ctttggtgag cactggctta atactattat tcgccagaca
    38521 ggatttaacc cgttcgaccg cgacgaagga ccgagcgtga aagcgacgca aattggcttc
    38581 gggcaattaa gccgcgcaca acaggtgctg ggaattggct atttgtatgt tgagacgcag
    38641 ttacgacaga tttgatggcg cgataacgta gaaaggcttc ccgaaggaag cctttttcaa
    38701 cagattaacc gtttcgctta tgcgtaaacc gggtaacgtg cgcagatgtc gagaacttta
    38761 cctttgatgc gctcgataac ggcttcatca ttgatgctgt ccagcacgtc acacatccag
    38821 ccagccagtt ctttcgcttc tacttctttg aagccgcgac gcgtaattgc cggagtaccc
    38881 acgcgaatac cggaggtcac aaacgggctc ttcggatcgt tcggtacgct gtttttgttg
    38941 acggtgatgt tagcacggcc cagagcggcg tctgcttctt taccggtcag gtttttatcg
    39001 accagatcaa ccaggaacag atggttatca gtaccgccgg aaaccacttt gtagccgcgt
    39061 tccaggaaca cttctaccat cgctttagcg tttttagcga cctgctgctg gtaagttttg
    39121 aactcaggct ccatcgcttc tttcagagca accgctttac cggcgattac gtgcatcaac
    39181 ggaccgccct gaccaccagg gaaaacggca gagttcagtt ttttgtacag ctcttcgcta
    39241 ccacctttcg ccaggatcag accgccgcgc ggacccgcca gggttttgtg agtggtggta
    39301 gtaacaacgt gagcatgagg aaccgggttc gggtagacgc cagcagcaac caggcccgca
    39361 acgtgcgcca tatcaacgaa caggtaagcg ccgatgctgt cagcgatttc acgcattttc
    39421 gcccagtcca ccacgccgga atatgcagag aagccaccga taatcatttt cggtttgtgt
    39481 tctttggctt gtttttccag atcggcgtag tcgatatgac cggtagcatc aataccgtaa
    39541 ggaacaatgt tgtacagttt accggagaag ttaaccggag aaccgtgagt caggtgaccg
    39601 ccatgcgcca ggttcatacc cagaacggta tcacctggtt ccagcagcgc ggtgtagacc
    39661 gcaaagttag cctgggagcc ggagtgcggc tggacgttag cgtagtcagc gccgaacagt
    39721 tctttcgcac gatcgatcgc cagttgttca acgatatcaa catactcgca accaccgtag
    39781 tagcgtttgc ccggataacc ttcagcatat ttgttggtca gctgagaacc ctgcgcctgc
    39841 attacgcgcg ggctggtgta gttttcggag gcgatcagtt cgatgtgctc ttcctgacgt
    39901 actttttcct gctccatagc ctgccacagt tcggcatcat aatcggcaat gttcatttca
    39961 cgctttaaca tccgcatctc ctgactcagc taacaataaa atttttggcc tttataggcg
    40021 gtcctgttgg acaacggcga acagtataac cgaatcattg tgcgataaca ggtcttgaca
    40081 aaggaattta cgcaaacgat taccttcagg ctacgcaagg ctttggagaa taaagagctt
    40141 gcaaccggaa acggatttct tttcaggttt gtgatgcaaa tttttcactt catcacattc
    40201 tttctgaaaa acaccaaaga accatttaca ttgcagggct attttttata agatgcattt
    40261 gagatacatc aattaagatg caaaaaaagg aagaccatat gcttgacgct caaaccatcg
    40321 ctacagtaaa agccaccatc cctttactgg tggaaacggg gccaaagtta accgcccatt
    40381 tctacgaccg tatgtttact cataacccag aactcaaaga aatttttaac atgagtaacc
    40441 agcgtaatgg cgatcaacgt gaagccctgt ttaacgctat tgccgcctac gccagtaata
    40501 ttgaaaacct gcctgcgctg ctgccagcgg tagaaaaaat cgcgcagaag cacaccagtt
    40561 tccagatcaa accggaacag tacaacatcg tcggtgaaca cctgttggca acgctggacg
    40621 aaatgttcag cccggggcag gaagtgctgg acgcgtgggg taaagcctat ggtgtactgg
    40681 ctaatgtatt tatcaatcgc gaggcggaaa tctataacga aaacgccagc aaagccggtg
    40741 gctgggaagg tactcgcgat ttccgcattg tggctaaaac acctcgcagc gcgcttatca
    40801 ccagcttcga actggagccg gtcgacggtg gcgcagtggc agaataccgt ccggggcaat
    40861 atctcggcgt ctggctgaag ccggaaggtt tcccgcatca ggaaattcgt cagtactctt
    40921 tgactcgcaa accggatggc aaaggctatc gtattgcggt gaaacgcgaa gagggtgggc
    40981 aggtatccaa ctggttgcac aatcacgcca atgttggcga tgtcgtgaaa ctggtcgctc
    41041 cggcaggtga tttctttatg gctgtcgcag atgacacacc agtgacgtta atctctgccg
    41101 gtgttggtca aacgccaatg ctggcaatgc tcgacacgct ggcaaaagca ggccacacag
    41161 cacaagtgaa ctggttccat gcggcagaaa atggcgatgt tcacgccttt gccgatgaag
    41221 ttaaggaact ggggcagtca ctgccgcgct ttaccgcgca cacctggtat cgtcagccga
    41281 gcgaagccga tcgcgctaaa ggtcagtttg atagcgaagg tctgatggat ttgagcaaac
    41341 tggaaggtgc gttcagcgat ccgacaatgc agttctatct ctgcggcccg gttggcttca
    41401 tgcagtttgc cgcgaaacag ttagtggatc tgggcgtgaa gcaggaaaac attcattacg
    41461 aatgctttgg cccgcataag gtgctgtgat ttgatgttgc cggatggaaa catccggcaa
    41521 cccttgacgc ggtttaaatt gccgcgtcgt cctcttcacc ggtacggatg cgaatgaccc
    41581 gtgccacgtc aaagacgaag attttaccgt caccgatttt gccggtttgc gccgtgcgaa
    41641 taatggtatc gacacaggta tcgacaatgt cgtccggtac gacaatctca attttcactt
    41701 tcggcagaaa atccaccata tactccgcgc cgcggtacag ctcggtatgg cctttctggc
    41761 ggccaaaacc tttcacctca gtgaccgtca tgccggtaat gccgacttca gccagtgctt
    41821 cgcggacatc gtccagcttg aagggtttaa taatcgcatc aatctttttc atgctattcc
    41881 ttgaaaaggt cgcctgtctt ttgatctgct aaacgtaaca cataacgcca attcattcct
    41941 tgaaatcgtt tgcatccagc tcgtgtcggg aaagcagttt ataaaattct gtccggttgc
    42001 gccccgccat tctcgccgcg tgggtgacgt tgcctttggt aatttgcagc agcttacgca
    42061 aatagttgag ttcaaactga ttacgtgcct caacaaaggt tggcagcgcc gtattttcac
    42121 cctccagcgc ctgctctact aatgcatcgc taatcaccgg agatgaggtc agcgccacgc
    42181 actgttcaat cacgttgacc aactggcgca cattacccgg ccagctcgcg gtcatcaggc
    42241 gtttcatcgc atcggtggaa aacgcgcgga caaacggttt atgtcgctct gccgcctggc
    42301 gcaacaggtg atttgccagt agcggaatgt cttctgtgcg ctccgccagt gccggaattt
    42361 tcaggctgac aacgttgagg cggtaataga gatcttcgcg gaattccccg cgcgccatcg
    42421 cttttggcag atcacggtga gtggcagaaa taatccgcac attgatatca atatcgcggt
    42481 tactgcccag cgggcgcact ttacgctcct gcaacacgcg caacagtttg acctgcaacg
    42541 gcgcaggcat atcgccaatc tcatcgagaa atagcgttcc gccttccgcc gcctggaata
    42601 aaccttcgcg attgctgaca gcgccagtaa acgcgccacg cgcatgacca aacagctccg
    42661 actccagcaa ttgctcaggc aatgcaccac agttaatagc aataaatggt ttgctattgc
    42721 gcgggctggc gttgtggata gcctgggcga aaatctcttt cccggtgccg ctctggccat
    42781 taatcaaaac gctgacgtct gattgcgcca ccagccgcgc ctgttccagc aaacgcagca
    42841 tcagcgggct gcgggtgaca atcgcctcgc gccagcgttc gtcggtggct ggcgcggatt
    42901 gttccagcgc atcatcaatt gcctgatata gcgcgtcttt atcaacaggc ttggtgagga
    42961 aactaaaaac gccctgctgt gttgcagcaa cggcatcggg aatagaacca tgcgcggtaa
    43021 gaataattac cggcattccc ggctgcactt tctggatttc agcaaacagc tgcataccgt
    43081 ccatttcatc catccgcagg tcgctgatga ctaaatctac tttttcgcga ttcagtaccc
    43141 gtaatccttc agcgccactt tccgccgtga ccacgctgta gccttcgctg gtcaggcgca
    43201 ggccaagcag tttcagcaat cccggatcgt catcgaccaa taataaatgc gcaggtttat
    43261 ggctcatcag gagtgacctc atgggtggat ggcgcgggct tttcactctc gtgtggcgta
    43321 tccggcgaga aatttccagc cggtttgcgg gtcgagagct ggcgttcaat atcagtcagg
    43381 ttttccagct tgcgggtggt gagttccagt tgctgctgta gaacgtgatg ttgctggcgc
    43441 aatgtatcca gctcgctgtc gctggactgc tggagtttac tgtagcgttg gcgctcttcc
    43501 gccagttgca gttgcagcgc ctgaccgtcg cgccagagtt gatacagtgg gcgaacctgt
    43561 gccgggatct cggtacttaa cgcttcaata cgcgcgacca gctggcggcg ctcatatggc
    43621 gtaattttgg cgtcggcgag caaaatccct tgtttaaagg tattttgcca gctgccgtcg
    43681 tcatattggc gagcttgctg acgcgactgc gcaggcatta aacgatcagc acaatccatc
    43741 gcccgcagcc agtaaagcgg attggtttcg gttgatttac cttgcagcgc ccagatgtcg
    43801 ctacattcag tagaaagata gtcagccagt tgataaaccg gaattttttc ttctgctggc
    43861 gtatcaatgg ctggcttatt gtgattctgc acgcaaccta gcaatgccag acatggcagc
    43921 cctgccagcc acagccgtcg gggcaataat cgttgaaaaa tgtgtcgcat attcaccaga
    43981 cttaaagcct atcccagtgg gcgtaattgt tgcagacagt ttggacatgg acagcgcgga
    44041 gaaaccggag cgtacatgtc gtacgtgagg atttcgagca ctgcccgggg ccgaactgac
    44101 aaataaaata gcctgatggg ataggctgtt agcattattt cgtgtttttc gacgacggta
    44161 attcaatgcg gaaacaaacg tcttgcccgc tctcgtcgac cagatacagt tccccttgca
    44221 tacggcgaat acaatccctg gcaatgctta atcccagacc gctgcccttc accgcccctt
    44281 ttcgctggtg gcttccctga aaaaagggtt cgaagatcat ggcgcgttcc tcttgcggaa
    44341 tgggcgtgcc tgtattgatg acatcaatat aaacccgcgc accatgtaaa ctgctgcgaa
    44401 ggcaaatgtt accggattca gccccgtagt gcaccgcatt ggagtaaaga ttatccagta
    44461 cgctcatcag cagcattggc tccgccaggc aagctgttgc tttgagatcg acgtcggtat
    44521 gcatcatttt agcccgtgcg ggcaggctat gagcagaaac tactgtctcc accagcggtg
    44581 ctaactcaac attatctagt tccaccgcac tgtccgcctg tttacggttg taatcaagca
    44641 gttgttcgat cagtttttgc aaattgcggc tgctgctatc aagaatgctc accacctctt
    44701 tttgctctgg cgtaagcggc ccgacaacct gatcagccag taattcagtg ccctcgcgca
    44761 tactcgccag tggcgttttt aattcatgag ataaatgtct taaaaattga tggcgttggg
    44821 attccagcca tgacaggcgc tcacttaacc aaagaatacg ttgcccaacc gagcgtaact
    44881 cgctcggtcc actgaacgaa acgctattgc ccagagaacg cccttccccc aaccgattga
    44941 tcatccgttc aatatttttc accggcccga taatcatccg cgtgaaaagc agtaccatta
    45001 ccagactcac cagaaatagc accagcgatt gccaaccaaa atattgccca cgttcggcga
    45061 tttcacgctg aagttgctgc ccacgagaga acactactgt gcgcgtggcc tgtaccattt
    45121 cggtattggc actggcaaag gcttccagac gcgcggcagc agcagcatcg ggaccactgt
    45181 tgttgcactg aagttgggcc agattgttca agtcctgacg caatgcctgg taaagtttat
    45241 cgtctggcag tacgcctgcg tgggcatcga gcatttcgct gtaacgcttg cgctggcttt
    45301 gataaacctt cgcaagcgta gggtcatcca gcacgcaata ctgacggtag ctacgctcca
    45361 tctccagcgc cgcgttggtc attgcttcgc tgcgccgggc atcgataagc gtggtgcggt
    45421 tgaccagcgc cgcctggtcg ctaagcgcat tcaggctttg ccaggcttgc catgccagca
    45481 ccaacagggg cagcagaatc agcaaaaatg ccagcattac cagttgtcgt aatgagcggg
    45541 gaaaaacggg ccagcgtttc aaggtgttac tctcgtcaga cgcgaatagc ctgatgctaa
    45601 ccgaggggaa gttcagatac aacaaagccg ggaattaccc ggctttgtta tggaataagg
    45661 cggtgcctaa ctcgacgttt cgcccgatgg ttgatatagc tacgctgata tcagaagttg
    45721 gacggcaggc accttgttgt gcgtcattcg aattttatgt agcacgtccc gaaggggctg
    45781 acataagtcg gtgaatgagc cactgattac tattatgcag taactgtgcc aataaagaaa
    45841 atagtttggt aacgtactga ttatacgttg ctttgagggg tttatggctt ccccgctatt
    45901 tgaatgtaaa tcagccactg tgtcgctaaa aagagacaac ttaagaaaaa cttattagat
    45961 tacaataaaa tcaagcattt aagtgtcgcc agaaagcgac acggtaaacc acccattgtc
    46021 gtgatttaca gacacaaaaa agctcccgaa gttgggagct tataatagcg gttgtattta
    46081 atgccggatg cggcgtgaat gcctcatccg ggctgcaaaa ccaatgagtt tgacggctta
    46141 ccccaactgc ttacgtgcat tgcggaaaat gcgcatccat gggccatcct cgccccagtt
    46201 ttcaggatgc caggagttgc tgacagtacg gaaaacacgt tccgggtgcg gcatcataat
    46261 ggtgactcga ccgctttcag tcgtgaccgc cgtaataccg ttcggagaac cgttcgggtt
    46321 agccgggtag gtttcagtga ctttgccgaa gttatcgaca tagcgcagtg ccaccagccc
    46381 tttgctttcc agtgccgcca gatgcgccgc atcacgcact tccacgcgcc cttcaccatg
    46441 agagacagca atcggcattt gcgagcccac catcccctgc aacagcagcg acgggctttg
    46501 ggtcacttca accaagctga aacgcgcttc aaagcgatcg gaggtattgc gcacaaaacg
    46561 tggccacaac tcactacctg ggatcagttc acgcagatta gacatcatct ggcaaccgtt
    46621 acataccccc agcgccagcg tttgcggacg gtggaagaag gttgcaaact catcgcgtac
    46681 acggtcattg aacaggatag acttcgccca accttcaccg gcacccagca catcaccgta
    46741 ggagaaacca ccgcacgcga ccagggcgtg gaaatcctcc aggcccgtgc gtccagccag
    46801 caggtcgctc atatgcacgt cgatagcatc gaagcctgca cggtggaaag ctgccgccat
    46861 ttcaacatgc gagttcacgc cctgctcacg cagcacagca actttcggac gtgcgccagt
    46921 ggcaatatac ggtgctgcca catcttcgtt gatatcaaac gacagtttca cattcaggcc
    46981 cggatcggcg tcgtttgatt tcgcctgatg ctcctgatcg gcacactccg ggttgtcacg
    47041 caggcgctgc atctgccagg tagtttctgc ccaccagaca cgcaacgtag tgcggctttc
    47101 gctgaataca gtctgcccgt tggcggtaat cacaaaacgg tcaccggaaa ccgcctgccc
    47161 aacataatgg acgcaatcag caagcccatg ctgtgccagt acggactcga ccgcttcacg
    47221 gtcagcggca cgaacctgaa tgaccgcacc cagttcttcg ttaaacaacg ccgccaggcg
    47281 atcgtcaccc agagtggcga tatccgcgtc aatgccgcaa tgaccagcaa aggccatttc
    47341 cgccagcgtt accagcagac cgccatcaga gcggtcgtga tacgccagca acttacgctg
    47401 tgcaaccagc gcctgaatcg cgtcatagaa gcctttcagt tgcgcgacat cgcgtacatc
    47461 tgccggtttg tcgccaagct gacgataaac ctgcgccagc gccgttgcgc ccagcgcgtt
    47521 attgcctttg cccaaatcaa tcagcagcag tgcgttatct tcggtagaaa gctgcggcgt
    47581 gatggtgtga cgtacatctt ccacgcgggc aaatgcagaa atcaccagcg acagcggcga
    47641 cgtcatttcg cgctcttcgt taccttcctg ccagcgggtt ttcatcgaca tagagtcttt
    47701 acccaccggg atcgtcaggc ccagcgccgg acaaagctct tcgcccacgg ctttaacggc
    47761 ttcatacagg cccgcatctt caccagggtg gcctgccgcc gccatccagt tggcggaaag
    47821 tttaatgcgt ttgatatcgc caatctgtgt tgcggcaata ttggttaacg cttcaccgac
    47881 cgccagacgg gcagaggcgg cgaaatccag cagcgcaacc ggcgcacgct cgccaatcgc
    47941 catcgcttca ccgtagtagc tgtcgaggct ggcggtagtg accgcgcagt tagcgaccgg
    48001 cacctgccac ggccccacca tctgatcgcg cgccaccatg ccggttacgc tgcggtcacc
    48061 gatggtcacc aggaaagttt tctccgccac ggtcggcaga tgcagtacgc gtttcaccgc
    48121 gtctgcaatg gtgatccctt cacggaccag cgcatcgccc ttcgctttca gcgtttgtac
    48181 atcgcgggtc atcttcggcg ttttaccaag caggacgtcc agcggcagat cgatcggctg
    48241 attatcaaaa tgacgatcgt gcagagaaag atgcagttct tcggtcgctt caccaatcac
    48301 cgcgtagggt gcgcgctcac gcttacacag ttcgtcaaac agcggtaatt gatcggcagc
    48361 aaccgccagc acgtagcgtt cctgggattc gttacaccag atttccagcg ggctcatgcc
    48421 cggttcgtcg cttagaatat cgcgcagttc aaatttaccg ccgcgcccgc cgtcgctcac
    48481 cagttccggc atcgcgttag aaagaccgcc agcgccgacg tcgtggataa acaggattgg
    48541 gttggcatca ccaagctgcc agcaacggtc gatcacttcc tggcagcgac gctccatctc
    48601 cgggttgtcg cgctgtacgg aagcaaagtc gaggtcggca tcagactgac ctgacgccat
    48661 ggaagacgcc gctccgccgc caagaccgat gttcattgcc gggccgccga gaacgaccag
    48721 tttcgcaccg acgttgatct cgcctttttg tacgtgatcg gcgcgaatgt tgccgatccc
    48781 gcccgccagc atgatcggtt tgtgataacc gcgcagctct tcgccgttgt ggctgttcac
    48841 tttttcttca taagtacgga agtagccatt cagtgccgga cgaccaaatt cgttgttaaa
    48901 cgccgcgccg cccagcgggc cttcggtcat gatgtccagc gcggtgacaa tgcgctcagg
    48961 cttaccgaaa tcttcttccc acggctgttc gaagccagga attcgcaggt tggaaacgga
    49021 gaaaccaacc aaacccgctt tcggcttcgc gccgcgcccg gtggcacctt catcgcggat
    49081 ttcaccgccg gaaccggtcg ccgcgcccgg ccacggagaa atcgccgtcg ggtggttgtg
    49141 agtttcgact ttcatcagaa tatgcgccgg ttcctgatgg aaatcgtagc ggcccgtttc
    49201 gtggtcagca aagtagcggc ccacttcaga accttccatt acggcggcgt tatctttata
    49261 agcagagaga acgtgatctg gcgtggtttc gaaagtattt ttgatcatct tgaacagcga
    49321 tttcggctgc tgttcaccgt cgataatcca gtcggcatta aaaattttgt ggcggcagtg
    49381 ctcagagttc gcctgagcga acatatacag ctcgatgtcg ttcgggttac gaccaagctt
    49441 tgtgaaagca tcctgcagat agtcaatttc atcttccgcc agagccaggc caagacgcag
    49501 gttagcgtcg atcagcgcct gacggccctg ccccagcaaa tcaacgctgg taaccggagt
    49561 cggttgatgg tgggcaaaca actgctctgc atcatctaaa gcaaaaaaga ccgtttccat
    49621 catgcggtcg tgcagttcag cggtaacctg ctgccattgt tcattggtca gcgtaccggc
    49681 ttctatatag taagcaacgc cgcgctcaag gcggtttacc tgttgtagcc cgcagttatg
    49741 agcaatatcg gtcgctttcg aagaccaggg agagatggtg ccaggacgcg gggtcaccag
    49801 caggagtttg ccttgcgggg cgtggctggc gagtgccggg ccatatttca gcaggcgttc
    49861 aagttgtgcg tgctcatcat cgtttaacgg cgcattgagg tcagcaaaat ggacatactc
    49921 ggcgtaaata ttgtgaaccg ggagcctggc agcctgaaaa cgtgccagca gtttgttgat
    49981 tcggaatgcc gacagtgcag gcgaaccacg cagaatttcc atcataagtc tctcgtcttc
    50041 taagctttcg gtgtacccaa ggggggaaac gggcgtcatt ataaagaatc tgatgcgctg
    50101 acgaaaccgt ttgcgtggaa ataaaatcac catcgtgaat tagcaacgcg tgccgccaat
    50161 ggctgtaata agttgccatc tggcgcaggt ttacgcaaaa tgccgctcat ttatgagtaa
    50221 acctttcact attattacat tttttcaagc tgggacgcgc acgacacaga gaattaacta
    50281 attgaaaaaa ttaaagatta attatctgtt catcggcatt ctggcactgc tgctcgcggt
    50341 cgctctctgg ccatccattc cctggtttgg taaagccgac aaccgtatcg ccgccattca
    50401 agcgcgggga gagttgcgtg tgagcaccat tcatactccc ctgacgtata acgaaatcaa
    50461 cgggaaacct tttggcctgg attacgaact ggcgaaacag tttgccgatt acctcggcgt
    50521 aaaactgaaa gtgaccgtgc ggcagaatat cagccagctg tttgacgacc tcgataatgg
    50581 taacgccgac ctgctggcgg caggacttgt ctataacagt gagcgggtaa aaaattatca
    50641 gcctggccct acctattatt ccgtgtcaca acaactggtt tataaagtgg gtcagtatcg
    50701 cccacgtacg ctgggcaacc tgacggcgga gcaactcacc gttgcaccgg gtcatgtggt
    50761 ggttaacgat ctccagaccc tgaaagaaac aaaattcccg gaattaagct ggaaggtaga
    50821 cgacaaaaaa ggctctgcgg aattaatgga agatgtcatc gaaggaaaac tcgattacac
    50881 cattgctgat tctgtcgcca tcagcctgtt tcagcgcgtt cacccggagc tcgccgtagc
    50941 gctcgatatc accgatgaac aaccggtgac ctggtttagc ccgttagatg gcgataatac
    51001 cctttccgcc gccctgctcg acttcttcaa cgaaatgaat gaagacggta cgctggcacg
    51061 cattgaagag aaatacctgg ggcatggcga tgattttgat tacgtcgata cgcgcacatt
    51121 tttacgcgcc gtcgatgcgg tactgccgca gttaaagccc ctgtttgaga aatacgccga
    51181 agaaattgac tggcgtttgc tggccgctat tgcttatcag gaatcgcact gggatgcaca
    51241 ggccacttca ccgacgggtg tgcgcggcat gatgatgtta accaaaaata ccgcgcaaag
    51301 cctcggcatt acggatcgta ccgatgccga acagagcatc agcggtggcg tgcgttattt
    51361 gcaggatatg atgagtaaag tgccggaaag tgtgccggag aacgagcgga tctggtttgc
    51421 cctcgctgcg tacaatatgg gctatgcgca tatgcaggat gcccgcgccc tgacggcaaa
    51481 aaccaaaggg aatcctgaca gttgggctga cgtaaaacag cgtctgcctt tacttagcca
    51541 gaaaccctat tacagcaagc tgacttacgg ctacgctcgt gggcatgaag cctacgctta
    51601 tgtcgaaaat attcgtaagt atcagattag cctggtgggt tatctgcaag agaaagagaa
    51661 gcaggctaca gaagcggcga tgcaactggc gcaggattat ccggcggtat cgcctacgga
    51721 gttgggcaaa gagaaatttc cttttctctc gtttctttcc cagtcgtcat caaactattt
    51781 gacccattct ccctctctgc tgttttccag gaaagggagt gaagagaaac aaaattaatc
    51841 cgtcgaggat tgcgcttttt tctgcgcttt aatttcctgg cggcgcatgc gaaagaagtc
    51901 actgagcaac gccgcgcact catccgccag tattccttcc gtaatttcca ctcggtgatt
    51961 catacccgga tgatgcagca catccattaa agatcccgca gcgccagttt tcgcgtcacg
    52021 cgcaccaaag accacgcgac caatgcgact gtggatcatc gctccggcac acattacaca
    52081 tggttcaagc gtgacataca acgtggcgtc gatcagacga taattttgca tcaccagacc
    52141 gccctgcctc agggccatga tttctgcatg tgcggtggga tcatgtcgac caatcggacg
    52201 gttccagcct tcgccaataa cacgattgtt atgcactaat accgcgccga ccggtacttc
    52261 ccgctcatcc caggcacgct tcgccagcgt cattgcatga cgcatccagt attcgtggct
    52321 aaattcgact tcagacaaaa agaaaactcc ggttataaaa gcgcggcgca ttatacacgg
    52381 acgctatgct ttactctatt ccagttgctg gagttcaccg cgcggggtta ctcgccagcg
    52441 atgctggcaa aagtaaagca gcgggttgtc ctgattactg tcgctatagc cactgtacag
    52501 ccgcagcggc gtgccgattt tgcgctccag ttgcgcgacc ttttcatgcc cgaggcaacg
    52561 catcgtcaat acccaaccac catagccacg ctgaatttgg ctggcgataa gattcacccg
    52621 cggcagccag ggtgtatcga aatagaccgc ttcaaccagc ggctgtggag aaccggtaat
    52681 caaccagata tcagcatcgg aacttaacag gtaggtggtt aatcgctcct gaaccagcgg
    52741 aaaggcggta acattgtcac gaaaccagcg tacgaaatcg gcctgcaacg cctgtaaacg
    52801 tgcttcgctg tgaccaaagg tgcatcccca cagaagcaga ctcatcggcc agcgtgccgc
    52861 acgacctttt atcaataacg caatggctat aatcggcaac aacggcagga caagtaacgc
    52921 attcagcggt tggcgacgca gtaaatagcg cagaaaactg ccgaacatat cctgctgatg
    52981 caatgttcca tctaagtcaa aaaacaccac acgacgctcg tgagttgcca aaccatactc
    53041 ctctggactg aaacatcctg attaattttc gctactacat agcctaacag atagatcatc
    53101 acttttccgg caacaatcga ccgcatcatg gctaactgga atttttaatt catggcaatt
    53161 agcggcaatg gaatataaaa ttcactcgcg tgtgtctcat attgcctgtt gtcgccacta
    53221 tttcatcgcg aggaaagata tgaacggctt acttcgtatc cgtcagcgtt accaggggct
    53281 tgcccagagt gataaaaaac tggcggatta tctgctgcta caacctgata cggcgcgcca
    53341 tttaagctct cagcaactgg ccaacgaagc cggagtcagt cagtccagcg tcgtgaagtt
    53401 cgcgcaaaaa ctcggctata aaggttttcc ggcgcttaag ttggcgttga gtgaagcgct
    53461 ggcaagccag ccggaatcgc cctcagtgcc cattcataac caaatccgcg gtgatgatcc
    53521 gttacggctg gtcggcgaaa aactgattaa agaaaatact gccgctatgt acgcaacgct
    53581 aaacgttaat agtgaagaga aactgcatga atgcgtaaca atgttgcgct ctgcgcggcg
    53641 gataattctg accggtattg gcgcttcggg tctggtggcg caaaactttg cctggaagct
    53701 gatgaagatt ggcttcaatg ctgccgcagt gcgcgatatg catgcgctac tcgctaccgt
    53761 acaggcgtcg tcccctgacg atctgttatt agccatttct tacaccggtg tacgacgcga
    53821 gttaaacctg gcggcagatg agatgctgcg agtgggcgga aaagtgctgg cgattaccgg
    53881 ctttactccc aatgccctgc aacagcgtgc ttctcattgc ctgtatacca ttgccgaaga
    53941 acaagcgaca aacagtgctt caatctctgc ttgtcacgcg cagggaatgt taacggattt
    54001 gctgttcatt gcgctgattc agcaggatct ggaactggca ccagaacgta ttcgtcatag
    54061 tgaagcgctg gtgaaaaaac tggtctgagt aaagaatgcg cgtataatgc ccgcccggtt
    54121 tgtgttgttt tgagagtttc cttatggcgc tgttaatcac taaaaaatgc atcaattgtg
    54181 atatgtgtga acccgaatgc ccgaatgagg cgatttcaat gggagatcat atctatgaga
    54241 ttaacagcga taagtgtacc gaatgcgtag ggcactacga gacaccaacc tgccagaagg
    54301 tgtgcccgat ccccaatact attgtgaaag atccgcaaca tgtcgagaca gaagaacagt
    54361 tgtgggataa atttgtgctg atgcaccacg cggataaaat ttaatattct actctggaag
    54421 tagagtatta att
//
LOCUS       DAZFAB010000022        53538 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZFAB010000022 DAZFAB010000000
VERSION     DAZFAB010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 53538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53538
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.022"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(142..2409)
                     /gene="adiA"
                     /locus_tag="ACHJWA_001507"
     CDS             complement(142..2409)
                     /gene="adiA"
                     /locus_tag="ACHJWA_001507"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFO4308137.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFAADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(2608..3516)
                     /gene="melR"
                     /locus_tag="ACHJWA_001508"
     CDS             complement(2608..3516)
                     /gene="melR"
                     /locus_tag="ACHJWA_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFO4308138.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACIPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3799..5154
                     /gene="melA"
                     /locus_tag="ACHJWA_001509"
     CDS             3799..5154
                     /gene="melA"
                     /locus_tag="ACHJWA_001509"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFO4308139.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYAPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYDAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            5257..6678
                     /gene="melB"
                     /locus_tag="ACHJWA_001510"
     CDS             5257..6678
                     /gene="melB"
                     /locus_tag="ACHJWA_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFO4308140.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIAFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKIPPEPVHADIPVGAVSDVKA"
     gene            complement(6817..7446)
                     /gene="yjdF"
                     /locus_tag="ACHJWA_001511"
     CDS             complement(6817..7446)
                     /gene="yjdF"
                     /locus_tag="ACHJWA_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFO4308141.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(7569..9215)
                     /gene="fumB"
                     /locus_tag="ACHJWA_001512"
     CDS             complement(7569..9215)
                     /gene="fumB"
                     /locus_tag="ACHJWA_001512"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO4308142.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSVETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHHPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIINKQCANCTK"
     gene            complement(9293..10633)
                     /gene="dcuB"
                     /locus_tag="ACHJWA_001513"
     CDS             complement(9293..10633)
                     /gene="dcuB"
                     /locus_tag="ACHJWA_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFO4308143.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(11204..11923)
                     /gene="dcuR"
                     /locus_tag="ACHJWA_001514"
     CDS             complement(11204..11923)
                     /gene="dcuR"
                     /locus_tag="ACHJWA_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFO4308144.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(11920..13551)
                     /gene="dcuS"
                     /locus_tag="ACHJWA_001515"
     CDS             complement(11920..13551)
                     /gene="dcuS"
                     /locus_tag="ACHJWA_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFO4308145.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            13732..13962
                     /gene="yjdI"
                     /locus_tag="ACHJWA_001516"
     CDS             13732..13962
                     /gene="yjdI"
                     /locus_tag="ACHJWA_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFO4308146.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            13974..14246
                     /gene="yjdJ"
                     /locus_tag="ACHJWA_001517"
     CDS             13974..14246
                     /gene="yjdJ"
                     /locus_tag="ACHJWA_001517"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO4308147.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            14473..14769
                     /gene="ghoS"
                     /locus_tag="ACHJWA_001518"
     CDS             14473..14769
                     /gene="ghoS"
                     /locus_tag="ACHJWA_001518"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO4308148.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            14797..14970
                     /gene="ghoT"
                     /locus_tag="ACHJWA_001519"
     CDS             14797..14970
                     /gene="ghoT"
                     /locus_tag="ACHJWA_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFO4308149.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(15089..16606)
                     /gene="lysS"
                     /locus_tag="ACHJWA_001520"
     CDS             complement(15089..16606)
                     /gene="lysS"
                     /locus_tag="ACHJWA_001520"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO4308150.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(16843..18300)
                     /gene="dtpC"
                     /locus_tag="ACHJWA_001521"
     CDS             complement(16843..18300)
                     /gene="dtpC"
                     /locus_tag="ACHJWA_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFO4308151.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESH
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMVSGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMVQESND"
     gene            complement(18359..20506)
                     /gene="cadA"
                     /locus_tag="ACHJWA_001522"
     CDS             complement(18359..20506)
                     /gene="cadA"
                     /locus_tag="ACHJWA_001522"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFO4308152.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(20586..21920)
                     /gene="cadB"
                     /locus_tag="ACHJWA_001523"
     CDS             complement(20586..21920)
                     /gene="cadB"
                     /locus_tag="ACHJWA_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFO4308153.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            complement(22286..23824)
                     /gene="cadC"
                     /locus_tag="ACHJWA_001524"
     CDS             complement(22286..23824)
                     /gene="cadC"
                     /locus_tag="ACHJWA_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HFO4308154.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSSLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSI
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            complement(24084..24243)
                     /locus_tag="ACHJWA_001525"
                     /pseudo
     CDS             complement(24084..24243)
                     /locus_tag="ACHJWA_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(24441..24516)
                     /locus_tag="ACHJWA_001526"
     tRNA            complement(24441..24516)
                     /locus_tag="ACHJWA_001526"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24481..24483),aa:Phe,seq:gaa)
     gene            complement(24623..25198)
                     /gene="yjdC"
                     /locus_tag="ACHJWA_001527"
     CDS             complement(24623..25198)
                     /gene="yjdC"
                     /locus_tag="ACHJWA_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO4308155.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            complement(25235..26932)
                     /gene="dsbD"
                     /locus_tag="ACHJWA_001528"
     CDS             complement(25235..26932)
                     /gene="dsbD"
                     /locus_tag="ACHJWA_001528"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFO4308156.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPVDQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQPT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLLTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFIMGTIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            complement(26908..27246)
                     /gene="cutA"
                     /locus_tag="ACHJWA_001529"
     CDS             complement(26908..27246)
                     /gene="cutA"
                     /locus_tag="ACHJWA_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFO4308157.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            complement(27362..28663)
                     /gene="dcuA"
                     /locus_tag="ACHJWA_001530"
     CDS             complement(27362..28663)
                     /gene="dcuA"
                     /locus_tag="ACHJWA_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFO4308158.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            complement(28781..30217)
                     /gene="aspA"
                     /locus_tag="ACHJWA_001531"
     CDS             complement(28781..30217)
                     /gene="aspA"
                     /locus_tag="ACHJWA_001531"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFO4308159.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            30554..31030
                     /gene="fxsA"
                     /locus_tag="ACHJWA_001532"
     CDS             30554..31030
                     /gene="fxsA"
                     /locus_tag="ACHJWA_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFO4308160.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            complement(31046..32302)
                     /gene="yjeH"
                     /locus_tag="ACHJWA_001533"
     CDS             complement(31046..32302)
                     /gene="yjeH"
                     /locus_tag="ACHJWA_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFO4308161.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPILIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            32578..32871
                     /gene="groS"
                     /locus_tag="ACHJWA_001534"
     CDS             32578..32871
                     /gene="groS"
                     /locus_tag="ACHJWA_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFO4308162.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            32915..34561
                     /gene="groL"
                     /locus_tag="ACHJWA_001535"
     CDS             32915..34561
                     /gene="groL"
                     /locus_tag="ACHJWA_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFO4308163.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            34699..35052
                     /gene="yjeI"
                     /locus_tag="ACHJWA_001536"
     CDS             34699..35052
                     /gene="yjeI"
                     /locus_tag="ACHJWA_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFO4308164.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(35244..36113)
                     /gene="yjeJ"
                     /locus_tag="ACHJWA_001537"
     CDS             complement(35244..36113)
                     /gene="yjeJ"
                     /locus_tag="ACHJWA_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFO4308165.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSP
                     LDFLPLYDVDCQQNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLVGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(36348..37376)
                     /gene="epmB"
                     /locus_tag="ACHJWA_001538"
     CDS             complement(36348..37376)
                     /gene="epmB"
                     /locus_tag="ACHJWA_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFO4308166.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RNAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRAGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            37418..37984
                     /gene="efp"
                     /locus_tag="ACHJWA_001539"
     CDS             37418..37984
                     /gene="efp"
                     /locus_tag="ACHJWA_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFO4308167.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            38036..38161
                     /gene="ecnA"
                     /locus_tag="ACHJWA_001540"
     CDS             38036..38161
                     /gene="ecnA"
                     /locus_tag="ACHJWA_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFO4308168.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            38272..38418
                     /gene="ecnB"
                     /locus_tag="ACHJWA_001541"
     CDS             38272..38418
                     /gene="ecnB"
                     /locus_tag="ACHJWA_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFO4308169.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            38594..38911
                     /gene="sugE"
                     /locus_tag="ACHJWA_001542"
     CDS             38594..38911
                     /gene="sugE"
                     /locus_tag="ACHJWA_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO4308170.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(38908..39441)
                     /gene="blc"
                     /locus_tag="ACHJWA_001543"
     CDS             complement(38908..39441)
                     /gene="blc"
                     /locus_tag="ACHJWA_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO4308171.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(39529..40662)
                     /gene="ampC"
                     /locus_tag="ACHJWA_001544"
     CDS             complement(39529..40662)
                     /gene="ampC"
                     /locus_tag="ACHJWA_001544"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO4308172.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIVNGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            complement(40725..41084)
                     /gene="frdD"
                     /locus_tag="ACHJWA_001545"
     CDS             complement(40725..41084)
                     /gene="frdD"
                     /locus_tag="ACHJWA_001545"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO4308173.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            complement(41095..41490)
                     /gene="frdC"
                     /locus_tag="ACHJWA_001546"
     CDS             complement(41095..41490)
                     /gene="frdC"
                     /locus_tag="ACHJWA_001546"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO4308174.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(41501..42235)
                     /gene="frdB"
                     /locus_tag="ACHJWA_001547"
     CDS             complement(41501..42235)
                     /gene="frdB"
                     /locus_tag="ACHJWA_001547"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO4308175.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(42228..44036)
                     /gene="frdA"
                     /locus_tag="ACHJWA_001548"
     CDS             complement(42228..44036)
                     /gene="frdA"
                     /locus_tag="ACHJWA_001548"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFO4308176.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTKRDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            44361..45338
                     /gene="epmA"
                     /locus_tag="ACHJWA_001549"
     CDS             44361..45338
                     /gene="epmA"
                     /locus_tag="ACHJWA_001549"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFO4308177.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            45557..47059
                     /gene="yjeM"
                     /locus_tag="ACHJWA_001550"
     CDS             45557..47059
                     /gene="yjeM"
                     /locus_tag="ACHJWA_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFO4308178.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(47210..50533)
                     /gene="mscM"
                     /locus_tag="ACHJWA_001551"
     CDS             complement(47210..50533)
                     /gene="mscM"
                     /locus_tag="ACHJWA_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFO4308179.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(50555..51523)
                     /gene="asd"
                     /locus_tag="ACHJWA_001552"
     CDS             complement(50555..51523)
                     /gene="asd"
                     /locus_tag="ACHJWA_001552"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFO4308180.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(51620..52672)
                     /gene="rsgA"
                     /locus_tag="ACHJWA_001553"
     CDS             complement(51620..52672)
                     /gene="rsgA"
                     /locus_tag="ACHJWA_001553"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFO4308181.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            52767..53312
                     /gene="orn"
                     /locus_tag="ACHJWA_001554"
     CDS             52767..53312
                     /gene="orn"
                     /locus_tag="ACHJWA_001554"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFO4308182.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDGWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
BASE COUNT        13288 a        13150 c        13632 g        13468 t
ORIGIN      
        1 aaaaagcgcg gggtggggcc gcgcaaaaag tatgacattt ttgtttgcag caatttttat
       61 gaaaacgttg ccatactcaa aaggcctaag gccaggcggc gtaatgttat ttaaacaatt
      121 acgccttcag cggaatagtg gttacgcttt cacacacata acgtggtaaa taccgtcaat
      181 aatttcagtc ccttcagttt cgtgttcaaa tccagggaaa tgatggtccc aggattgcag
      241 cgagcgtaaa taacttactt gcggactgtt tttatcgccg aagttttcac cagacagcag
      301 catcgggatt cctggcggat acgggataac tgagtttgcc gcgatacgtc ctggcagatt
      361 ttcaatggat accagttcga cattgttgtc gacaatcgcg ttgtacgctt cacgcggggt
      421 gatttccgcc accggcaggc cggaataggc ttcgttcaac cgtgcgccag ggttgttttc
      481 tttcagccag gcaaacatgg tgtcacccag atcgtgaatc cccatgttcg cgtaagtgtc
      541 aggatattgt tcaacaagtt ccggcatcac ctgcgccagc ggtgtgttgg cgtcatagtg
      601 gcgtttgaag gagcaaaggg tgttaaccag agtcccccat ttcccacggg ttacgcccat
      661 agagaacagg aacataattt ggaagtcagt ggtgcgggta ggcacaatgc cgtggcgacc
      721 aagccaggca gtgaccagcg ccgccggaac accggtttct tccagttcac catcttcacc
      781 cattcccgga gcaaggatgc tgactttaat cgggtcgagc atactccagt tatccggaat
      841 atctttgaag ccgtgccagc tttcgcccgg atgcattacc cagcagtcct gaacggtggt
      901 cagcagtttg gttggtgcgt cagcaaagtc ataggttttg ccggtttgtg ggtcggtgac
      961 gacttctttg ttccacggtt tgaagaacca gctaccgtca gcggcgaact ctttatacag
     1021 ccgcgccatc gcctgacgga aatcaaccgc ttcgtcgatc acttcctgcg tcagtgacag
     1081 gccgctgttg ccgtccatca tcgacaccgc cacgtcgttg gatgcgcaga tggcatacag
     1141 cggggaggtg gtggcatgca tcatgtaggc ctggttgaag cgggagaagt taatcgcccc
     1201 acgaccttca cgtacatgaa tataagaggc ctgtgaaagc gcattcagca gtttgtgggt
     1261 ggagtgggtg gcgaaaacgg taggaccgtt gtgatcgcca ggttcgccgc gcatggcata
     1321 gtgatcggca tagatcgggt tgaaacgtgc atagccgtac caggcttcgt caaagtgcag
     1381 acgatcggag gttttttcca gcagatcctg cgcttcttta gcgttataac acacgccgtc
     1441 ataggtgcag ttggtcacca cgcagtaaga cggtttttgc ccggctttgt ctttagtcag
     1501 cgggctttca ctgattttct tctgcaaggt ttcaggttgc atttcctgcg gatagattgg
     1561 cccgataatg ccgtagcggt tgcggcttgg caccatgtag accggtttcg cgcctgtcag
     1621 catcaaacct tgttcgatgg atttatggca gttacggtca acgaccacga catcgttatc
     1681 ggtcatgcaa gcctgcatga tggtgcggtt agagccggaa gtaccgacga ctaccgacca
     1741 ggagcgatcg gcaccaaata cgcgtgcggc atatttttcg ctttcgccaa atgcgccagt
     1801 atggtcaagc aaagaaccga gggaagttcg ttcgatgccc atgtcggtgc ggaacagatt
     1861 ttcaccgtag tagtcatggt agaaacgtcc ggcgggtgtt ttggtaaaac caacgccgcc
     1921 ctggtggcct ggcgctgccc aggaatattc atggatgtcg ctatatttca tcagcgcgct
     1981 gaacagtggc ggcaacagct gctggcggta gcgggtcatc gcggcaacgg cgcgtccggc
     2041 gataaagtcg gcggtatctt ccagaatcca ggcgaattca tcgacaagct ccagcagatc
     2101 ccggtccatt gcggcgaggg ctttttcccg atcgcccaac aggaagaccg gcacgttttg
     2161 ttggcgctca tgaagcttac cgatcaattg tctgacgttt tgatgttcgt ccggatgttc
     2221 catttgatag ctgaacatca ggcagtcaat ggcttcgttt gaagagagaa tggcaaaacc
     2281 atcatcaaag gaggtggatt taatcacggt aacattttgc tggcttaaag catctgccag
     2341 acgctcaaca gcgttaccga cccaggtgtc ttgatggaga aactcgcttt caacaattaa
     2401 tactttcatc attgcttacc cggttatgaa ggaatcttcg ttgcgggcaa gtatcttccg
     2461 gccttttcgg gctgtaaagc gcgtgaaaaa caaacatttt tgaaatattt gtttttataa
     2521 acaatatgtt atgtgattat tttaaattat tttcactgct tattatcgac ggctaaacta
     2581 tttttttggc tgatactgat atcgtcttta gccgggaaac gtctggcggc gctgttggct
     2641 aagtttgcgg tattgttgcg gcgacatgcc gacatatttg ccgaacgtgc tgtaaaaacg
     2701 actacttgaa cgaaagcctg ccgtcagggc aatatcgaga atacttttat cggtatcgct
     2761 cagtaacgcg cgaacgtggt tgatgcgcat cgcggtaatg tactgtttca tcgtcaattg
     2821 catgacccgc tggaatatcc ccattgcata gttggcgtta agtttgacgt gctcagccac
     2881 atcgttgatg gtcagcgcct ggtcatagtt ttcggcaata aagcccagca tctggctaac
     2941 ataaaattgc gcatggcgcg agacgctgtt tttgtgtgtg cgcgaggttt tattgaccag
     3001 aatcggttcc cagccagaga ggctaaatcg cttgagcatc aggccaattt catcaatggc
     3061 gagctggcga atttgctcat tcggactgtt taattcctgc tgccagcggc gcacttcaaa
     3121 cgggctaagt tgctgtgtag caagtgattt gatcaccatg ccgtgagtga cgtggttaat
     3181 caggtcttta tccagcggcc aggagagaaa cagatgcatc ggcagattaa aaatcgccat
     3241 gctctgacag gttccggtat ctgttagttg gtgcggtata caggcccaga acagcgtgat
     3301 atgtccctga ttgatattca ctttttcatt gttgatcagg tattccacat cgccatcgaa
     3361 aggcacattc acttcgacct gaccatgcca gtggctggtg ggcatgatat gcggtgcgcg
     3421 aaactcaatc tccatccgct ggtattccga atacagcgac agcgggctgc gggtctgttt
     3481 ttcgtcgctg ctgcacataa acgtatctgt attcatggat ggctctcttt cctggaatat
     3541 cagaattatg gcaggagtga gggaggatga ctgcgagtgg gagcacggtt ttcaccctct
     3601 tcccagaggg gcgaggggag tgtccgagca tcatgaggcc gaaaactctg cttttcaggt
     3661 aaattattcc cataaactca gatttactgc tgcttcacgc aggatctgag tttatgggaa
     3721 tgctcaacct ggaagccgga ggttttctgc agattcgcct gccatgatga agttattcaa
     3781 gcaagccagg agatctgcat gatgtctgca cccaaaatta catttatcgg cgctggttcg
     3841 acgattttcg ttaaaaatat tcttggtgat gtgttccatc gcgaggcgct gaaaacggcg
     3901 catattgccc tgatggacat tgaccccacc cgcctggaag agtcgcatat tgtggtgcgt
     3961 aagctgatgg attcagcagg ggccagcggc aaaatcacct gccacaccca acagaaagaa
     4021 gccttacagg atgccgattt tgtcgtggtg gcatttcaga ttggcggtta tgcaccttgc
     4081 acggtgactg atttcgaggt ctgtaagcgg catggtctgg aacaaaccat tgccgatacg
     4141 ttggggccgg gcggtattat gcgcgcgcta cgtaccattc cgcatctgtg gcaaatttgc
     4201 gaggacatga cggaagtctg ccccgatgcc accatgctca actatgttaa cccaatggcg
     4261 atgaatacct gggcgatgta tgcccgctat ccgcatatca aacaggtcgg gctgtgccat
     4321 tcggtgcagg gaacggcgga agagctggcg cgtgacctca atatcgaccc ggctacgctg
     4381 cgttaccgtt gcgcaggtat caaccatatg gcgttttacc tggagctgga gcgcaaaacc
     4441 gccgacggca gttacgtgaa tctctacccg gaactgctgg cggcttatga cgcagggcag
     4501 gcaccgaagc cgaatattca cggcaatact cgctgccaga atattgtgcg ctatgaaatg
     4561 ttcaaaaagc tgggctattt cgtcacggaa tcgtcagaac attttgctga gtacacaccg
     4621 tggtttatta agccaggtcg tgaggatttg attgagcgtt ataaagtacc gctggatgag
     4681 tacccgaaac gctgcgtcga gcagctggcg aactggcata aagagctgga ggagtataaa
     4741 aacgcctccc ggattgatat taaaccgtca cgggaatatg ccagcacaat catgaacgct
     4801 atctggacgg gcgagccgag tgtgatttac ggcaacgtcc gtaacgatgg tttgattgat
     4861 aacctgccac aaggatgttg cgtggaagtg gcctgtctgg ttgatgctaa tggcattcag
     4921 ccgaccaaag tcggtacgct accttcgcat ctggccgccc tgatgcaaac caacatcaac
     4981 gtacagacgc tgctgaccga agctattctt acggaaaatc gcgaccgtgt ttaccacgct
     5041 gcgatgatgg acccgcatac tgccgccgtg ctgggcattg acgaaatata tgctcttgtt
     5101 gacgacctga ttgccgccca cggcgactgg ctgccaggct ggttgcaccg ttaaaacgcg
     5161 actaaacgct actgcgccgg gggatttatt ccggcgcaca cctctgacga taccaataac
     5221 agaaggcggg cgttggtaac agcgacccga taccctatga gcatttcaat gactacaaaa
     5281 ctcagttatg gatttggagc gttcgggaag gattttgcga tcggcattgt gtatatgtac
     5341 ctcatgtatt actacaccga tgtcgtcggg ctgtctgtgg gtttggtcgg tactttgttt
     5401 ctggtggcga ggatctggga tgctattaac gatccgatta tgggatggat tgtaaatgct
     5461 acgcgatcgc gatggggtaa gttcaaaccc tggatcctga tcggtacgtt ggcaaactct
     5521 gtaatcttat ttctcctctt tagtgcgcat ctgtttgaag gtactactca gattgccttt
     5581 gtttgcgtga cctacatcct ctggggcatg acttacacca ttatggatat tcccttctgg
     5641 tcgctggttc caaccatcac gctcgataaa cgtgagcgtg aacaactggt tccttatccg
     5701 cgtttttttg ccagtctggc gggctttgtt acggcaggtg tgacgctacc atttgttaat
     5761 tatgtcggcg gtggcgatcg gggatttggc tttcagatgt tcacactggt actgatcgcc
     5821 ttttttattg tttcaaccat catcactctg cgcaatgtgc atgaagtctt ttcgtcagac
     5881 aatcaaccgt ctgctgaagg aagccatctg acacttaaag ccatcgttgc gcttatttat
     5941 aaaaacgatc agctttcatg cctcttgggt atggctcttg cttataatgt agccagcaac
     6001 attattaccg gctttgctat ctattatttc tcatatgtta tcggtgatgc ggatttgttc
     6061 ccctattatc tgtcgtatgc gggagctgct aacctggtga cgttagtatt cttcccacgc
     6121 ttagttaaat cattatcccg acgcatttta tgggccggag catctattct tccggtgtta
     6181 agctgtggtg ttctcctgtt aatggcatta atgagctatc acaacgtcgt cctcattgtg
     6241 attgcgggta ttttgctgaa tgtgggaacg gcgcttttct gggtattaca ggtcatcatg
     6301 gtggcagata ccgttgatta cggtgaatat aaactgcacg tacgctgtga aagcatcgct
     6361 tactccgtgc agactatggt ggtgaagggc ggttcagcct ttgcggcttt tttcattgcg
     6421 gttgtgttag ggatgattgg ctatgtaccg aatgttgaac agtctacgca agccctatta
     6481 ggtatgcagt ttattatgat tgctctaccg actctgtttt tcatggtaac gctgattctc
     6541 tacttccgtt tctatcgcct caatggcgac acgctgcgca ggatccagat tcatctgctg
     6601 gataaatatc gcaaaatacc gcccgagcct gttcatgctg atattccggt cggtgcagtg
     6661 agtgatgtga aagcctgacg tggacaatag ctaacgaaaa tggcctgatg cgatatgttt
     6721 atcaggccac gtcttaatgg aatgctttgg atttgcaaat tttgtagacc ggataaggaa
     6781 ttcacgccgc atccggcatc aacaaagtgc acgttgttat ccggttatca agccaaagcg
     6841 ccgtagctga cggcaatgaa agcgagcgag gaatatcacc gtggttaatg cgccaagcag
     6901 cgcgcagaac atatcggatt gcgtgtccca ctggtcgccc tgggtgccga gaaaatcatc
     6961 cgctccctgg cccattgcca gcgccgccca ccactcgatt aattcataca tcgcgcttat
     7021 cgccagcgcc acgcaacaga ccagaaacgc caccatttta cgtccgcgaa cgtacatccc
     7081 gcgcacaagg atctctcgtg ccaccagtgc aggcaccagc ccctggaaaa aatgccccag
     7141 cttgtcatac ggattacggc tcaaccccag ccattcctgc acctcaaaac cgatggggac
     7201 tttcgcgtag gtgtattgcc cgccgaccat caggatgatg gcgtgaaaga aaatgagcgt
     7261 atagagcagc ggcgttaacg gataacgttt agcggtggca agcagtagcg gcacgacaat
     7321 aatcaccggt gtcacttcca tcagccaggt gagtttgtca tgggccgaaa tgccggtata
     7381 aatcaggatt aacgttagcg ccagtgcgcc ggtgttaaga attaacggct tgagtgtgcg
     7441 ggtcatggtg agttcgcaag tcagggaaaa tactgactat tcaccagcaa gcgtgaaatt
     7501 acaatcgcaa aagaggtggc ccagggggat cacctggcag catgctgcca ggcgctgggc
     7561 cgaagaggtt acttagtgca gttcgcgcac tgtttgttga tgatttgctg gaagaagtcg
     7621 ttacctttgt catcgaccag gataaacgcc gggaaatctt ctacttcgat tttccagata
     7681 gcttccatac ccagctccgg ataagcgacg cattccagat gcttgatgct ctgctgcgcc
     7741 agtaccgccg ccggaccgcc gatgctaccg aggtagaagc cgccgtgttt atgacacgcg
     7801 tcggtaacct gctgactgcg gttacctttc gctagcatga tcatgctgcc gccgtgggat
     7861 tgcagcagat ccacatagga gtccatacga cctgcggtgg ttgggccaag tgagcctgat
     7921 ggataaccgg caggggtttt cgccggaccc gcgtagtaga tcgggtgatc tttgatgtac
     7981 tgcggaagtt ctttaccggc gtcaatcagc tctttcagct tggcgtgtgc aatatctcgg
     8041 cccacgataa tggtgccggt gagcgacaaa cgagtggaaa ccggatattg cgaaagctgg
     8101 gcgaggatct ctttcatcgg gcggttaagg tcgaccttca ccgcttcgcc ttcaccggcc
     8161 tggcgcagtt cttgtggaat gtactgacct ggatggtgtt ccagtttttc gatccagata
     8221 ccttcgcggt tgattttcgc tttaatgtta cggtcagcgg agcaggagac gcccatgcct
     8281 accgggcagg atgcgccgtg acgtggcaga cggataacgc gaatgtcgtg cgcgaagtat
     8341 ttaccgccaa actgcgcgcc aagaccgagt ttctgggcct cttccagcag ttcctgttcc
     8401 agctggacat cgcggaacgc ctgaccatgt tcgttccctt ccgtcggcag ttcatcgtaa
     8461 tagtgagcgc ttgctaactt aacggttttc aggttggttt ccacagacgt accgccaatc
     8521 acaaacgcga tatggtacgg cgggcaggct gcagtaccga gggtacgcat tttctcgacg
     8581 aggaagtttt tcagtttgcc gggagtcagc agggctttgg tttcctggta gagataggtt
     8641 ttgttggcag agccgccgcc tttcgcaacg caaaggaatt tgtactcatc gccatctacc
     8701 gcgtacaggt cgatttgcgc aggcaggtta gtgccggtgt tgacctcttt gtacatgtcc
     8761 agcgccgcgt tttgcgagta gcgcaggtta tcttcgatat aggtgttata gacgcctttc
     8821 gacagcgctt cttcatcacc gccaccggtc cacacgcgct ggcctttttt accgacgatg
     8881 atcgcggtgc cggtatcctg gcaggtcggc agcacgcctt tggcggcgat ttcggagttt
     8941 cttaagaatt gcagtgccac gtacttgtcg ttttcgctgg cttctggatc gtgaagaata
     9001 gccgcaacct gtttctggtg tgccggacgg agcatgaaag aagcgtcgtg gaaggcttgc
     9061 tgcgccagca gggtcagggc ttctggttcc actttcagga tggtttcgcc gtcgaagtcg
     9121 gcaacgctaa cgtaatcgga agtgagtaga tagtattcgg tattgtcttt ccccatcggg
     9181 aaaggtgcct ggtagataaa gggtttgttt gacatagctt ccagcctgta actctgtatt
     9241 tgttattcga aaatggcgtg ccgcgtagta acgggcggca cgcaaagtgc atttataaga
     9301 acccgtacat cgcggcgaag atccagccga agacgcacga tacgctcaca ccaatcaacc
     9361 ccggcagaat aaagctgtgg ttgatgacga agcgaccgat gtgggtggtg ccggaacggt
     9421 caaactgaat cgctgccaga tcgctcggat aagtcggcag gatgtaataa ccgtagcaag
     9481 ccggtgctga agccacgatg tatgccggat caacgccgat cgctagcgcg accggaacaa
     9541 tcgccgccag cgccgcagcc tgagagttta caaacttgga aaccagcagc agaacaatgg
     9601 cataggccca cggatactct ttcaccattt cacccagtac gccctgaatt tcagacatat
     9661 gcgcaccgaa catggtttct gccatccatg cgataccgta caccgccacg atggcgatca
     9721 taccggaacg gaagacttcg ttttttgaga tagacgcggg attggttttg gtcaggataa
     9781 taatcagcgc cccggtcagc agcataaaca tctgaataac cagtaccatc gacagcggtt
     9841 tgccgccgaa ggatggacgc aggtccgaat cagcaccaag aagtgcgact acagcgattg
     9901 ccccgaggaa aatccacatt gccagccagt tgcttttcgg cagtttttta tccagcagcg
     9961 tcgcggtatc accgtaaaca tactcacggt tttccggtac ggagatgaat ttctggaact
    10021 cttcgtcttt atccagatct ttaccgcgga accagctgaa gataccgatc gccaggatac
    10081 cgattaacgt cgatggaatg gtgattgcca gcaggtcgag gaactcaaga tggcgaccat
    10141 caaaggtgac attacccagc attgcaacca gagacacgac cgcaaccgac accggactgg
    10201 cgataatccc catctgtgca ccgatagaac ttgccgccat cggacgttcc ggacggatgt
    10261 tgttcttaat ggcgacgtcg tagatgatcg gcagaatggt gtaaaccaca tgacccgtac
    10321 cgcaaagaat ggtcagcgta caggtcacaa acggcgcgac aattgagaca tatttcgggt
    10381 tgcggcgcag cagcttctcg gcaatttgca gcatgacatc aagaccgccc gaagcttgca
    10441 aggtcgccga tgccgccacc accgcaatga taaccagcat gacatcaact ggtggtttac
    10501 ctggctgaag gtggaagacg aagaccagaa tgaccagacc gataccgcct aataaaccca
    10561 gcgcgatacc accctttctg gcaccataaa acagacatat cagtattatg ataagttgga
    10621 tagtaaataa catgtgtgaa ccctcgcgat aatcctattt aaatttttgc tgaatagatc
    10681 acagtcacgt tctgttttgt atgaactgtt ttcagagcct gactaaatat ccgtttggtc
    10741 gttaccggct ttagcaaata cctcacagtg aatattggct gattaaagca gcggcaaatt
    10801 tatgcactgt gataagcggc tttttcagag agaaaaactc tctggcgatg ggtttaaagt
    10861 ttgtactgcg cagatgttaa tgttttgttt gctgttatct tattgatatt tatcggtcta
    10921 ttttttgttc tcagggcaga tatcattagg tttatagatt aatctgatct acccatttgt
    10981 gggtaaaaat acacataatg cgggtgacat aatagttaat taacttttgt tagcgttttg
    11041 aaattaaaaa caccgttcac ctgaagagat attaattttt agcgatgatg gagggataat
    11101 tatatttgat ctggcacaag ttttactgat gaaggatgta acttgtacca ggggtatttt
    11161 gcattacggt aataattatt acttacagat aaccagcgat ccgttattgg caatattgtt
    11221 tcagtagtga gtagtgttct gcctgaatac ggtaacggta aactggacgc cccgtgacgc
    11281 cataatggat actggtgaac aagatgtggc agttgaccag ccagataagg tatttacggc
    11341 aggaaacacg cgaaatgtta acctcgttgg ctagctcgtc ggttgaaaac tcatagtcct
    11401 gatgcgcgtc aatccactgg cacagtgtgc gtaacgtctg cggcgttaag ccttttggca
    11461 agcgacgagg atcctgctcg ttggagctgc tgccgtggat tagctgatca agctcggcct
    11521 ggtcataata ctgatgtttt tccagcgcca ttttcttttg ccgccagccg gtgagcgcct
    11581 cttcaaagcg ggaagcctgg aagggtttga tcaggtaatc cacgacaccg taatgcagcg
    11641 aatctttaat ggttgccgca tcggctgcgg aggagatgac aatcacatca cttttgcaac
    11701 gcgcgttatg caggacaggc agtaaatcga gcccgttctc tttttgcata tagatatcga
    11761 gcaatatcag gtcgataggc gtatcgctat tgaagataat ctctttggct ttctccagcg
    11821 tcgaggctgt tccacagcat tgaaagcctg ggatttgtgc tacgtatcgg cgattcagct
    11881 ccgcgaccat tgcgtcgtca tcgataatta atacattgat catctgttcg acctctcccc
    11941 gtcccagggt atctggacaa aaaattgtgt gaaaatcccg ggttccgatt ccacggcgat
    12001 gctgccgccg agattttcta cctgttgttt gacaagtgct aaaccgacgc ctcgctcgct
    12061 tccttttgtc gagacacctt tgtcaaaaat gtgatcgatt ttgtcgggtg cgatccccgg
    12121 tccatcatcg ttaacttcgc agtgcagcca gccgtgacgg tagtgcaatg ttacgctaat
    12181 ttcgccaccg ggttccggcc ctaatgcttc cagcgcgttt tctatcagat ttcccaatgt
    12241 ggtaatcagc gtcgcgacct ggtcctcact gccgctgtct ggcagctggc tttcactgtt
    12301 taaaatcagc gtatggccta aatcggtcgc gcggttaatc ttgctgatta aaaaaccagc
    12361 gataaccgga gatttgatct tacccagcag agagccaatc tcttcctgat agttattggc
    12421 tgttttgaga atgtaatctt ccaactgctt ataactcttc agatgcaata atccgagaat
    12481 cacatgcaat ttattcataa attcgtggga tcgttcacga agtgcatcag catagttgac
    12541 cagaccgtcg agtcgctgca tcagtttacg aacttcagtt ttgtccctga aggttgaaat
    12601 ggcaccgatg ataacgccat tactgcgcac cggaacggtg ttgatcagta atagccggtc
    12661 tttaatcgta atctcttcgt cgcggcgcgg cgtaccgtcg cgtaacactt ccgagacatc
    12721 taccacctgt gaccatgagt ggctgagcgt cgacagtttc tcatcgtcct gcgacttacg
    12781 gtaattcagc aattcttgtg cggcatcgtt gatcagcgtg acctcgccgc gatcgtccac
    12841 ggcaacgacg ccttctttga tagactgcaa catggcctgg cgttgctcaa acagcgtgga
    12901 gatttcgtag ggttccaagc cgaaaaggat ttttttcagt accttaacca gaatgcaggt
    12961 gccaatcagt ccgaccagca tgccaaataa taccgaccag ataatgctcc agcgactgtc
    13021 attgatctgt tgggtcacgc ggcttaactc aaggccgatc gccaccacgc caatttgttt
    13081 atgattttca tcgtagatgg gggtaaatac gcgtaaagcc tgcgccagaa aaccgcgatt
    13141 gatagcgaca ttttcttcgc cattcagcgc tttaaggatg tcatcacctt taaatggctg
    13201 accaatacgc tgggcttcag gatgcgagta gcgaagactt tgcatatcgg taacgacaat
    13261 aaacagcaga tcgttgcgtt tgcgcacggc ttccgcgatg gcctggatgc cgctctcctg
    13321 cggttttttc tgcaagccct gacggatttc cggcgagtcg gcgagagtac gcgccactgc
    13381 cagtgccttg ttggctagcc cgtctcgcgt catatcactg atttgcgaga agtaaatcag
    13441 atgcaccacc aatagcaccg agaacagtac cgcgctgacc attaagatca ctgtggtact
    13501 caatttcatc ggacgtttgc gtaacatgcg gtagggcaat gaatgtctca tcagcttcct
    13561 tgtgtgacaa atttcttaag cattatctct gatgaggcgg gtaattcaaa gggagtaaga
    13621 atgattggct atatagggga agagactctg gcaacggaaa ctgccagtgc tgtatgaaga
    13681 ttccagggct atgcttatag tgaaaaccat actgatgaga gagggaaggt catggatcag
    13741 gcgctactgg acgggggtta tcgctgttat accggcgaaa agatcgatgt ctatttcaac
    13801 actgcgatat gtcagcattc tggcaattgc gtacgtggca acggcaagtt atttaatctc
    13861 aaacgaaagc cgtggatcat gccggatgaa gtcgacgttg ccactgtggt taaagtgatt
    13921 gatacgtgcc cgagtggtgc gctgaaatac cgtcataaat aagcgagggt aaaatggaaa
    13981 tacgcgaagg ccacaataaa ttttacatta atgacgaaca aggcaagcaa atcgctgaaa
    14041 ttgtctttgt gccgaccgga gagaatttag cgattatcga acataccgat gtcgatgaaa
    14101 gcctgaaagg gcaagggatt ggtaaacagc tggttgcgaa agtcgtggaa aaaatgcgtc
    14161 gggaaaaacg aaaaattatc ccattatgtc catttgcgaa acacgaattt gataaaacgc
    14221 gggagtatga tgatattcgc agttgatgga agagtacaga ttcacgatat ttttcatgct
    14281 ctccgcggtg tgatgcagga gagcatctga agggtagggg gatgcacaaa gaatgggcag
    14341 agagcgcgtt tttttgtccc aagtcatccc ctttactgag caaaaaaaag aatatctcct
    14401 atatgagaat catcaatcgg ggttaataaa ttttgcgtcc ccagagtgtt caatatttat
    14461 aggagtcata ttatggaagg taaaaacaag ttcaatactt atgttgtttc ttttgattat
    14521 ccatcatctt attcctcagt gttcttaagg ttaagatcat tgatgtatga tatgaatttc
    14581 tcttctatcg tggctgatga atatgggata ccacgacaat tgaatgaaaa ttccttcgca
    14641 ataacgacat cgttagccgc aagtgaaatc gaagatttaa tcaggcttaa atgcttagac
    14701 ttaccggata ttgactttga cctcaacatt atgacagttg atgactattt ccgtcagttt
    14761 tacaagtagc cagtaaggat aagaagaaca taaaaaatgg cactattctc taaaatatta
    14821 attttttatg tgattggtgt gaacatatcc tttgtcatta tctggtttat ctcacatgag
    14881 aaaacacata ttcgtttact tagtgcattc ctggtcggaa taacctggcc aatgagtctg
    14941 cctgtggcat tacttttttc tctcttttag gagctgtagt tgagtggacc cgctggttat
    15001 cgtgaaacga agcgtctctc ctcaaaactg gatttgcgga acgatgaacg aagcagtcgc
    15061 ctgactgctt cgttcattaa agtgaaattt atttctgtgg gcgcatcgcc gggaagagaa
    15121 taacgtcgcg aatagtatgg ctgttagtaa acagcataat cattcggtcg ataccaatac
    15181 ccagaccagc ggttggcggc agaccatatt ccagcgcagt cacgtaatct tcgtcataga
    15241 acatggcttc gtcgtcacct gcggctttag cgttaacctg ttcctggaaa cgttcagcct
    15301 gatcttctgc gtcgtttaat tcgctaaaac cattaccgat ttcacgacca ccgatgaaga
    15361 attcaaaacg gtcggtgatt tccgggttaa catcattacg gcgtgccagc ggtgacactt
    15421 ctgccggata ttccgtaata aaggttggct gaatcagatg tgcttctgcc acttcatcaa
    15481 agatctccgt gacaatacgt cccagccccc agcttttctc taccgtaata ccgatagatt
    15541 cagctaatgc tttagcagca tcaaaattat ccaggtcggc catatcggtt tctggacgat
    15601 attttttgat tgcttcgcgc atggtgagtt tttcaaacgg tttgccgaaa tcaaacacat
    15661 gctcgccata agtgacttta gtggtaccca gaacctcttg tgccagggtg cggaacaggg
    15721 actctgtcag ttcaatcaaa tcgtggtaat ccgcatacgc catgtagagt tccatcattg
    15781 tgaactcagg attatggcga acagaaatac cttcattacg gaagttacgg ttgatttcga
    15841 atacccgttc aaaaccgcct acaaccagac gtttcagata cagctccggc gcgatacgca
    15901 gatacatatc taaatccaga gcattatgat gggtaataaa cgggcgagca gatgccccac
    15961 ctggaattac ctgcatcatc ggggtttcta cttccataaa gccgcgcgcg accatgaact
    16021 ggcggatagc ggccagaatt tttgaacgga caacaaacgt ttgacgggat ttatcgttag
    16081 cgatgaggtc cagataacgt tgacgataac ggacttcctg atcctgcaga ccatggaatt
    16141 tatctggtaa aggacgtagt gctttagtca gcaggcgcaa ctcagtacag tgaatggaaa
    16201 gctcacccgt ttgcgtctta aacagcgtac cgcgggcacc gataatgtca cccagatccc
    16261 attttttaaa ctgatcgtta taaacacctt ctggcaggct atctcgtgcg acgtacagtt
    16321 gaatacggcc accgacatcc tgcaacgtta caaaggaggc tttccccatg atacgacggg
    16381 tcatcattcg gccagcaacc gagacttcaa tgtttaagga ttccagttcc tggttatcct
    16441 tcgcatcaaa ctcttcgtgc aactggtcag aggtatggtc gcggcgaaaa tcattgggaa
    16501 acgccacacc ttgctgacgt agtgccgcca gtttttcgcg gcgatttctc agttcatcgt
    16561 taaaatcaat agcctcattg gctccccgtg tttcttgttc agacattttg gttcctctaa
    16621 atccagcttt caatttagcg tagataaaga gacagatcgg tacactgtat tatctgcctc
    16681 gactattaat aactcagttt atcttatcga aactaacgct tacctcaagt tgatatcact
    16741 aataaataac catcaaaatg gtttaaatga cttattaatc tcgatttact agtgtgataa
    16801 actgaaagtt aatttattca taaccaattg tttttacgac agttaatcgt tgctctcctg
    16861 taccatattc gtcggcgtgc taaacaggaa acgggtagca aaggccaata ccacgatgat
    16921 cgcgacacaa gccaacgtcc actctcccat ctgagaaaag aatcgctggt aagctgcaat
    16981 tgccataccg ctaatttgcg actctgtcgt ctgctgtgcc actacgcctg ccagccagtt
    17041 ggcgaccgcg cctgttgcca gcatataaat cccggttaat acgccagaca ttttcagacg
    17101 cgtgatttgc gcaatcgcca ccgggtcaat aaagagttcg gcaaagccca ttagcgccag
    17161 cccggatacc atcacgccca ttgacgcttg accgtcagct gctgcatgtc gggcatcaaa
    17221 tgccagcaac ataaagccac aagccatcag cagtaagcca aaggcaaact tcagccagac
    17281 gcgcaatgtt gagttgccgt ggctttctgg cgacgccagc caggccagaa caaccccagc
    17341 gagcatcacc gcaattgcat tcaccgactg gaatagtgca gtaggtactt caatattgaa
    17401 tgcctgacga ttcacaaagc gatcgataaa caagctgatg gtactgccgc cctgttgtgc
    17461 cagtacccag aacaatgtcc cgacaaacat caacaataca atttgccaaa gagcacggcg
    17521 gtgttcgggg aatttgatca tcatgcgggc aatgatttgt gcggcaatga ggcaaacgat
    17581 cgccagcaaa tatccggacc agtcgttctc cagcagcagg gtaaaaaata ctggggctaa
    17641 acagagcatc accactaacc agctccatac cggtaaggca aatttgacgc tagtgagcgc
    17701 ttttttatcc atactacgcg tggactggaa atgacgatgc ccgcttaaga aaatcaacaa
    17761 accgataaac atgccgccac ccgcaagggc aaagccaaca tgccatccat accactgagc
    17821 agccaggccg caggcgattg gggcggcgat agaaccgata ttgcccgcag catacagcag
    17881 cgaaaaaccg ccatcacgtc gatgatcgtt ctcgtcgtag agctcgccaa gcaaacagct
    17941 gatgtttgat ttgaataaac cgtagccaca aataatgatt gccagcgcca gatacaggct
    18001 aaaggttgaa tttgtatcaa tacccagcac cacatggcca agggtcatta acagcgcgcc
    18061 ggcaatcact gcagtgcggt tgccgagcag gcggtcggca agccagccgc cgagaatagg
    18121 ggtaacgtaa accagagaag catatgcgct gaacaggctg atggcatggt tatcatcaaa
    18181 accaagctga tgggtgagat agagaatgag taaggcacgc atgccgtaaa aactgaagta
    18241 ctcccagatt tggatcgcca cgatatagta tatcgcgcgc ggctgtgagg gtgttttcat
    18301 gtgttctcct tatgagcaaa aaagggaagt ggcaagccac ttcccttgta cgagctaatt
    18361 attttttgct ttcttctttc aataccttaa cggtatagcg gccatcagcc tgacggtatg
    18421 caccgtgaat atcggtttca aagcccggat agtgagcgcc gatttcacac agcatctgca
    18481 ggaactccag aaccggacgg ctttcttcgg tgatcatttc acccggcatt accagaggaa
    18541 ctcccggcgg atacggaagg atcatattgg cgttaatacg accgaccatt tcgtcgaggt
    18601 aaacttcttc ggtcataccg tgcagctctt tctggaatgc agcatacgga gtcattacca
    18661 tcgtcggcag cacttcaaat gcgcgataca tcagatccgg cagattgtgg tgaacaatca
    18721 gtttgtggat attctgagcc agttcctgaa tacgcatgtt ttcatagaat tcaggatctt
    18781 cacgatacag agacggcagc atgtttttca cacgcaggtt caggtcgaac gcacgtttga
    18841 agtcagtcag agcacgcagc aggctcagtg ctttggtctt atcgataccg atgctgaaca
    18901 ggaacagcag gttatacgga ccggttttct caacaacgat gccatgttcg tcgaggtatt
    18961 tcgccacgat gctggccgga ataccaaagt cgctcatggt gccgtctttt tccatccccg
    19021 gagtcagcag ggtgactttg atcgggtcaa gatacatgtg ctcgttatcg atgtttttga
    19081 agccgtgcca ggtgctgtca gaacgcagcg gccagcattc agtcgtatcg atatgatccg
    19141 gctgccatac atcaaagaac cagccatcag attccgttct cagacgtttg atctctttac
    19201 ggaatttgat cgcacgttca atggaaccgt tgatcagacg cttacctgca ttgcctttca
    19261 tcatcgccgc agcggtttca gtggacgcca cgataccgta gtgcggagaa gtggtggtgt
    19321 gcatcatgta ggcttcgtta aaggtttctt cgtttacgtc acctttaacg tggatcatgg
    19381 aagcctgaga gaacgccgcc agcagtttgt gagtggactg ggtttcgtaa atcactttcc
    19441 cttctacacg gccaccgctc ataccgcatt taccttcgta aatcggtgag aagttggtgt
    19501 aaggcaccca cgcggagtca aagtggatgg atttcacatc cagtgttttc ttgatgaagt
    19561 cggtgttgta cagcagacca tcataggtag agttggtaat tacagcatgt accggccagg
    19621 ttgcgtttgg tgtttctttc acgcgcttag caatggtagc gtgctggaat tcactctgtg
    19681 ggataccacc aagaataccg taagcgttac gggtcgggcg gaaatagatt ggcgtaacat
    19741 cgctcatcat catcaggtgg gtcagcgatt tgtggcagtt acggtcaatc agaatggtgc
    19801 tgcctgccgg agcagagtac ataccaacaa ttttgttcgc agtggaagta ccgttggtca
    19861 ccatgtagct gcggtctgcg ttaaagacgc gagcgatata ctgttctgct tctttgtgtg
    19921 gaccactgtg atccagcaga gaacccagtt cagatactga aatggaaata tcagatttca
    19981 tggtattcgg accaaagaaa tcatagaaca ggctacctac cgggcttttc tggaatgcag
    20041 taccacccat gtgaccagga gtacagaaag tatatttacc ttcacgaaca tatttaaaca
    20101 gtgctttagt cagcggaggc agaatagtgt tgatatattc gtcagtggtc tgcttgatct
    20161 tgttagcaat atcttcagca gcacccagcg catattcaaa gaagctaatc tgtaaacgca
    20221 ggtcattcag gcttacatcg agagtggaat acgtattagc gaacgcgtac aacggcaggt
    20281 tctcgttcat tttgctaatt tcttcgcaca gctcgagatt atatttatcc cagtcaaaaa
    20341 taacgccgca cagacgcgca ttgttttcga tcagttttaa taagtcgtca cggtcgttcg
    20401 ggtaaacaat ctggaagttc agacgttcaa gcgcgcgatg aagttcacgg atgggttctt
    20461 ctttaaaata aacccccatg tgattcaata ttgcaataac gttcatagtc atatctccag
    20521 gtaaaaaagg cccctcccaa cacatgggac aaaatgaaag gaggagcctc ggaaaatact
    20581 tttaattaat gtgcgttaga cgcggtgtgg ttatccattg agtggctctg gcgctcgtgc
    20641 attttgcgag cgtagaacat caggataatc aggctgacga tgaaggtacc tgccagctcg
    20701 aaggagcttg cgcccatcag cgcgatgaag cagaacacgc aacccagtac agagcagatc
    20761 aggctgacaa agttgcggat gttaacgcct tcaaaacgaa tcaggtcaac gcaagagtag
    20821 aagtacggca gcatagtcag cagtactgcg ataccggtca gttcaccgaa caggtcagat
    20881 gctttaccac cggcagagtt catcagagtg atgaggatca tcagggcagt cattttcact
    20941 gcagccagca gcagaccttt tttcggaata ccgttgctgt cgacttcacc ataaactttc
    21001 gggaagttac cgtcgttagc ggcacgtaca cctgcctggc ctaccaacat catccaggag
    21061 cccagagaag tcaggcacgc aaaggcggtg aatgcagaaa ccagcggcgc agcccagtta
    21121 ccgaggatag ttgaagcact gattgcaaac ggagcaccgg aagccgccat tacagaagac
    21181 ggatacatac cggaaagcac ctgagtcgca gcgatgtaaa caatacctgc taaaccagta
    21241 cccagcatgg ttgccagcgg aacggtacgt ttcgggtttt taaccatacc agtacttaca
    21301 gctgcggatt caacacccac gaaggcccac aggcagagca gaatactttt aatgatcgca
    21361 tgaccatcag tggtatccgc agtattccag ttagctgcat aagttgccgc atcaaaccaa
    21421 tgccagccaa caatagcagt catcaccaca ggaataagaa ccagcaccag accaatagtg
    21481 gttaaacggc ttacccaggt accgccgagc atatttacaa aggtaaatac ccagacgata
    21541 gcaatacagg cgatacccgc cggaacagga tcatttaata ctgggaagaa ggtggaaaga
    21601 taagatacag cggtaatacc aatcgccagg ttaccaatcc agttagcatg gtaataaaga
    21661 acacctgtct gaaaaccaaa tgcaggagaa atttctccgg cataagcaat tgggccacct
    21721 tgttgcgggt tttttgttgc cagtcgggca tatacatacg ccagcgacat tgcaccaata
    21781 atagagataa tccaacccca gatagcaata ccaccgatac ttgctaggtt cgcaggtaat
    21841 aatgcaatac cgctccccat catattaccg gcaacaacac cggtacaggc aaatagcccg
    21901 atcttcttgg cagaactcat gctcttctcc taatttcatt tttgaatttg gagtccgggt
    21961 catgatgtat aactatttcc tgaccagacc aaactggcgc taagattact cacgaaaaaa
    22021 ggattaatcc taaagattag gtgaaataac acaaaagttt ctgtaagtga gaacttgagg
    22081 ttttttatta acacatcagg atcgcaagtt gatatcatga aaagataaac attcaatgtt
    22141 tacaatagat tgcgtgacgt cttctggtta aatttatgta ataaaaatta tgcggcaaat
    22201 aaattgccgc aacatattat accaacagga acatacaaaa actcaacaac aaatattccc
    22261 gagcatgaat caacccgggg gttacttatt ctgaagcgag aaatttgtcg agataaggta
    22321 caacataagg aacagaagtc tggaatatac cattttcaat ccagtaaagg gtgtttgccc
    22381 ctgggcgtaa attaaaggcg gtgagatatg catcagccgc ttcccggttc atccccttca
    22441 tttcataaac cttgccaagc aatacataat ttagccagga catttcaaga tcaatgccag
    22501 tatttatcgc ctggtaagac tcatctgttt taccttttac cagagcactg accgctttta
    22561 tttgatatat aatggacagg ttattcaatt ccggtagtgt aacaatgtta tctatttctg
    22621 tgttcagtgc tgctaattgt ttttcatcta aaggatgttg agaatggcgc acgatatcaa
    22681 ctaatgcttt ttctgctctc gcgtaggtaa attctgggga tgattgaaca atctcaccta
    22741 ataattcact ggcacggttc aatgatttat catcgccatg cagtaaataa tcatgtgcct
    22801 gataaaaatt agttaataac gcaccacgat gaggcaaaat ttgctggagc gtctcctgca
    22861 ttcgttgtgg ccacggttgg tttaacgctt ttgataaact ctccagtaaa tcattttgaa
    22921 tcgccagctg attaccgtta gtgatgacat aacgtttatc cagcatggtt gaaccatctg
    22981 cattgtctac caattttatc gacataaagc attgttgagc acggtattgg cgctgattaa
    23041 caaacgcaat agataatgtt ttaccggaac tgctcggttc atcaatgttg tagttgattt
    23101 tgtcatgcac cataaaggtg gagaaggtgt taagtgatgt cgccaccaaa tcacccacgc
    23161 ctatcgcgta agagagctga tacggggaac tccagctgtt acaactttta tttaccatat
    23221 taatgtcaat atcgcgtgga ttgagcaaaa tacgcgattt gctcatcgga agacgtgtat
    23281 caagacttga aaacgctacc agtgctacac agatacctaa ggacaacagg aaaaaaaacc
    23341 atacccaaaa ggtagtgaat cgtttgcttt taactgggga ttgttcaggt ggcgttgcgg
    23401 tgttttgaat gttaagactg tgggagggag aatctgtggc aggaaccgcc tctggtatag
    23461 ggggaggcga agatagcatt atttcctctc cctcttcttc gctgtaccag ataaccggca
    23521 ccattaattt atagccgcgc tttggtacag tagcgatata gacaggacta tcttcatcat
    23581 tatcttttaa tgacttacgt agttctgaga tactctgcgt cacaacgtga ttggtgacaa
    23641 tacttctctt ccagacatta tcgataagtt catccctgct aagtacttcg ccactgtgtt
    23701 gagcaaagaa aaccagaagg tcgattaatc tcggctcaag ggtaagttga cgcccattgc
    23761 ggctaatttg gtttatggac ggagtaacaa gccattcgcc aacgcgaact acaggttgtt
    23821 gcataataga aactcattcg aaaagggaat gatgcaatga taattgccac aacctatttt
    23881 taccatctat agatgggttt atttacatat tattggtgaa tgcaagacgt tatttttacc
    23941 agccataaac ttcttgatta catggtatta cgaaaggatt ttactgagaa ccagaagtaa
    24001 tattccttac catcaaaatt catcatcttt gccaaagaaa aatgttcaga aaataatcca
    24061 tggaaaattg tccggagcgc ttactatttt aatggattgt tagtctttgc atgagcaagc
    24121 ggactgatac atttctcttt gttctcattc agaaaatctc atcagtcggc gttcagaccg
    24181 atgttgcgct ttatggatgc gttacgctcc tgatgacgtc atttgacgtt caacagcatc
    24241 acggggccgc acgacatttc acgttagtta gtgctatagc tcaggaacaa attttcccga
    24301 attgggatat gcccgcaaat tgctggtgat gtggggagaa tcgggttgag ttcggtagaa
    24361 ttgatttgga ggcagaacac ttaaatcgtg gcgtcctgaa acgaaaaacg gacctccgtg
    24421 gaggtccgtt tatatgaatt tggtgcccgg actcggaatt gaaccaagga cacggggatt
    24481 ttcaatcccc tgctctaccg actgagctat ccgggcaacg gggcgcatta aaccgtaatc
    24541 tgcacatctc gtcaacctaa tttcagtaaa agcgattcaa ctgcttaaga ttgcggcaaa
    24601 tcgcttcttt tctgtgtttc tgtcaggtca gtgcaccacc ctggcggcag cgggcgaagc
    24661 gcaggatatc ttccgccagc cgatgtgcgg tgtcgacatc cgcctggcta cgattcacca
    24721 gcattcggct taaacagcct tccagcacca gttccatctg ctttgctacc atcgccggat
    24781 cgtcaacttc cagcgtggtt aacagttcgt gggtgaaatc gtaggccgcg cttttttgct
    24841 gatcggccag ttgatgaata gggtggccag gatcgggata aaacgtacag gcagcgataa
    24901 acagacagcc cggatagcgg ttgtttttaa cgcactccga taacgcctga taacgtgcca
    24961 gcagcttttg ttcggcggtt tgcgtttcgt ccagcatcag ctgacgacgc cagacatcta
    25021 tctgttggct aagataacgc agcgcatcgt agaggattgc ctctttgtct ggccagaagc
    25081 ggcgtagctc gtccagtgga taatccacac gttcagcaac catctccagc gtggtgttgg
    25141 caatcccttg taattctaat aatttcaggg cttctcccag tacatcttca cgttgcacgc
    25201 tattttcctc cgtctttccc actgcaagtg tcgttcacgg ttggcgatcg cgcaaatgtg
    25261 cgctgaaggt ttcagcatcc ataaagcccg tgacgcgtgc ttgtggatgc tcctggcctt
    25321 gtccgtcaaa aaagagaatt gtcggtaggc caaggacatt aagatgcttt aacagcgcca
    25381 catcttgtgc gtcgttggca gtgacgttgg cctgaagtaa taccgtgtct gctaacgctt
    25441 tttgcacctg cgggtcgctg aaggtgtatt tctcaaactc tttacaggcg acgcaccagt
    25501 cggcataaag atctaacatc accggtttgc ctttggcttc aacgagcgcc tgatttaact
    25561 catctaccgt tttgatttgt gtaaagttga gatgcgtctg agtttgcgcg gtatgcgtcg
    25621 caccaaatgc ccaatcctga agtgggcgca cgctaaccaa tgccgctgca agcaggatta
    25681 tttgcaccac gcgcatccag ccgcgtttgg cctgtaggct ggtgataaag gcccagccaa
    25741 agaatgcgac acccagcgcc gaccacaagc gtaatcccca tacatcacca atcactcgct
    25801 ccagcaggaa gaccggcagt gcgaggatca caaaaccaaa cgcggttttg acttgttcca
    25861 tccacgggcc gcttttcggc agcaagcggt taccaaagac ggtaattagc atcagcggca
    25921 ggcccatgcc caacgcatag agataaagcg tgccgccgcc cagccacatg ttcccgcttt
    25981 gggcgatata cagcagaatc gcgctaagcg gtgcggtggt gcatggtgaa cagatcagtc
    26041 cggcaatcgt ccccataata aacactccgc cgggtgagcc gccctgttgg cgattgctca
    26101 tcagcgtgag gcgcgtttgc agcgaggagg ggagttgtag agtaagcaag ccaaacattg
    26161 acatcgccag caaggtaaag acgatggcga ggccaatgag cacgtatggg tgctgtagcg
    26221 ccgcctggaa ctgtaaccct gcggcggcaa ctaccagacc cagcgccgtg taagtcagtg
    26281 ccatcccctg cacataaata aaggtcagca acaatgctct ggcagtggag agccgctgtt
    26341 taccacccag cacgatgcca gaaatcagtg ggtacattgg cagcacgcat ggcgtaaagg
    26401 cgataccaat accgatcaac aacgcccaga gcgcggaaaa gggcaattgc gcggtgggct
    26461 gctcttgctg cggaacagac acaggctgtg acgctgcgtt gttggcgacc acttcgctta
    26521 acggaacggt tttggtttct ggcggataac agaaaccggc atcagcacag ccctggtagg
    26581 tgacagttaa cgtcgctccc gcactcgcct ggttgatggt tacgggaagc gtcagccgat
    26641 cgcggtaaat ctcgcttttg ccgtaaaact catcttcatg ccagacgcct tgcggcagct
    26701 gcacgtcggc aattttcgcg tgttccggcg taatgcggat ctgtttacgg tagaggtagt
    26761 aaccgtcttt gatctgccag gtcagattaa ggtcatgttg gttttgctga aaatcaaaag
    26821 caaaggcttg atccacgggg acaaattgtg aacgtcccgg cgcgtcgaat aatccggcaa
    26881 aaacggaagt gctgcaaagt agcaggatca gcgtaaagat gcgttgagcc atgagaggta
    26941 atctgtgtct ccgtgtgtaa caggtaaaac cagaagttcc ggggtttgat atggatgatg
    27001 agacttcagg cattccagca gtgcctgctg gtgagatacg gtagttttta aaatcatctg
    27061 cacttcgtat tcttgctcca gcttaccttc ccagtaatag agagaggtag cgccggggat
    27121 caaggtcgcg caggccgcca gtttttccgc cagcactttg gcggctaaat cctgggctgt
    27181 cgcttcatcc ggtgcggtac atagcaccac gacagacgcg gtattcgaac ttttttcatc
    27241 aagcataaac acctcgcaag aacagatgga accgcaagag agaggtcact atacaacgga
    27301 cggggaaagg atgttagtcg gcgagaaaga aagcggggcg tgaacgcccc gcgattggtc
    27361 attacagcat gaagctaccc agcacgaagc cgaagcaaac ggccagggca acacccagag
    27421 tacccgggat gaagaacgga tggttgaaga cgaatttacc gatacgggta gtacccgtgt
    27481 catccatctg taccgcagca accagcgtcg ggtaggtcgg cagaatgaac agaccagaca
    27541 ccgcagcgaa agaagcaaca gcggtcagcg gagaaacgtt cagtgccaga gccatcggca
    27601 tcagtgcttt tgcggttgca gcctgagagt acagcagagc agaagcaaag aagaagatga
    27661 cggccagcag ccacggatga ccctgaatca cttcaccagc ggtatctttg atccagtcga
    27721 tgttgttgga aacgaaagta tcgcccagcc acgcaacacc caggatacaa atacaggcgc
    27781 tcatacctgc tttgaaggtg ctggagttga ggatgttgtc ggtatcgact ttacagataa
    27841 cggtggtcag agttgcaaca ctgagcatga tgatcaggat tgcgttggtg gtgttcatca
    27901 gcggtttttc aaccagaccc atgcttgggc tgttgatgat tgcatagata accacgccaa
    27961 ctacgcccag caggaacagc cagacggacg tttttgcacc ggatttgatt tcaatctgct
    28021 tttcaccgcg cagttcaacc aggccctctt ccagacgctt gcgataaatc ggatcgtcag
    28081 agagtttgga gttgaacagc atagtgacca ggaaggacat caccagaacc gccagcaggg
    28141 tggacgggat gaccacggag agcagatgga ggtagctgat gccatgacct tccatcacgg
    28201 aagacatgta aaccactgcc gctgagattg gcgatgcggt gatcgcaatc tgcgcggata
    28261 ccactgcagt agacagcgga cggcaaggtt taacgccttg ttccttcgca acttcagcga
    28321 taactggcag tgtcgccaga gagatgttgc cagtaccagc aaagatagtc aggaaatagg
    28381 tcacgatcgg tgcgaggatc gtgatgtatt tcgggttacg gcgcagcagc ttttctgtct
    28441 gatgaaccag atagtccaga ccgccagcaa cctgcatggc agaaatagcg gcgataaccg
    28501 ccatgataat ggaaatgaca tcgaacggga tgttaccggg tttaacgcca atagcggcaa
    28561 gaaccagcac ccccaatccg cctgcaaaac caatacctat tccccccaat ctggcgccca
    28621 agaagatcgc cagcaaaact atgatgagtt ctacaactag catattagcc ttccttgttt
    28681 tttaacaagt tgatattaga ttgttatttt taagttactg ctcacaagaa aaaaggcacg
    28741 tcatctgacg tgcctttttt atttgtacta ccctgtacga ttactgttcg ctttcatcag
    28801 tatagcgttt tgctttgtaa gccgggtgca tcagattctg tacggagaaa atatcgtcaa
    28861 gttccgcttc agtcaacaga ccgcgttcca gaacgacttc acgtacactc ttaccggttt
    28921 cggcacagat tttacccacg atgtcaccgt tgtggtgacc gatgaacggg ttcaggtaag
    28981 taacgatacc gatagagttg taaacgtaac cttcgcacac ttctttgtta gcagtgatgc
    29041 cgttaatgca tttttccagc aggttgtagc aagcgttggt cagaatgtga acggattcga
    29101 acatagcctg gccaatgacc ggctccataa cgttcaactg cagctgacct gcttctgctg
    29161 ccatggtaac agtggtgtcg ttaccgatga ctttgaagca tacctggtta accacttccg
    29221 gaacaaccgg gtttacttta gctggcatga tggaagagcc cgcctgcagt tccggcaggt
    29281 tgatctcgtt caggccggca cgtggaccag aagagagcaa gcgcaggtcg ttacagattt
    29341 tggacatctt cacagccagg cgtttcagcg ctccgtgaac cataacataa gcgccgcagt
    29401 cagaggtcgc ttcgatcagg tcttcagccg gtacgcatgg gaagccagta acttcagcca
    29461 gttttttcac tgccagcgga gagtactctt tcggcgtgtt cagaccagta ccgattgctg
    29521 ttgcgccaag gttaacttcc agcagcagtt cagcggtacg ttggatgttt ttcacttctt
    29581 ctttcagcag gatgctgaaa gcgcggaatt cctgaccgag ggtcatcggt actgcgtcct
    29641 gcagctgggt acgacccatt ttcaggatgt cctggaattc gaccgcttta cgttcaaagc
    29701 cttcacgcaa ttggttaatc gcatctacca gcttaatcag ggaagagtaa actgcgatac
    29761 ggaaaccggt cgggtaggcg tcgttagtgg actgacattt gttaacatgg tcgttcgggt
    29821 tcaggtactg atattcacct ttctggtgac ccatcagttc cagaccgata ttggccagca
    29881 cttcgttggt gttcatgttt acggaagtac ctgcgccgcc ctggtagacg tctaccggga
    29941 actgatccat gcattttccg ttgttcagga cttcatcaca tgcggcaatg atggcattcg
    30001 ctacactttt aggaatggtt tgcagctctt tgtttgccat agctgcggct tttttaacca
    30061 ttaccatacc gcgaacaaat tcaggaatat cactgatttt gttgttgctg atatagaagt
    30121 tttcaatcgc tctcagagtg tgaacaccat agtaggcatc agctggaact tccctggtac
    30181 ccaacagatc ttcttcgata cgaatgttgt ttgacatgtg aaccttcttt ttcaagctgc
    30241 caatgatttg ctttaaacac acagaatata tgtggtttcg aatgtttttc gaccgacgat
    30301 tatcccctgc atcgaccgaa tatccgagat catatgctgc ttgaggattt ctaccgtaat
    30361 ctggatcact ttaagtgtcg gtttttaccc cttaattatt aatttgtgaa atagatcacc
    30421 gctttgggat tactaccaaa aatagttgcg caaatatctt gaaattttgc taatgaccac
    30481 aatataagct gaacgcgatt cgcaacccat tcaggtagcc gggtttaacc ggctgctatt
    30541 acaggagaaa cctttgcgct ggttaccttt tattgccatt ttcctttatg tctatattga
    30601 gatttcaatc tttattcagg ttgcccatgt attgggggta ttgctgactc tcgtgctggt
    30661 tatatttacg tcagttatcg gtatgtcact ggtacgtaac cagggcttta agaatttcgt
    30721 gctgatgcag caaaaaatgg cggcgggtga aaacccagcg gcggagatga ttaaaagtgt
    30781 ttcgctgatc attgccggtt tgctgctttt attaccgggc tttttcaccg acttcctcgg
    30841 tcttctgctt ttattgccgc cggtgcaaaa gcatctgaca gtgaagttga tgccgcattt
    30901 gcgcttttct cgcatgccag gcggcggttt tagcgccggg accggtggcg gtaatacttt
    30961 tgatggtgag taccagcgaa aggatgatga ccgcgaccgc cttgatcata aagacgatcg
    31021 ccaggattaa tgtcgaaacg ccggattatg tggttatgcc attttccggc gtttttcgtt
    31081 ttggcagaaa cagccataac cccgccagca tgatcagcgc atagagactt ttccagccga
    31141 ccattgccag taacagaacg cataacagcc cgccaaccac cgccagtagt cgataacgac
    31201 cttgcaataa tttacagcct gccagcatgc ataacagata aatcataata aagatgccat
    31261 tggcataaat aataagagcg tccagattga tctctaaagc atgaatcacc aacgtgctca
    31321 ccacgcagca gccgagcacc gcattgaggg cattattcgg gatatggcga gaagagaggc
    31381 gtgccaggta gtggtcagga ttatgttgcg cctgcgacca gaccaggcgg gcgaagctct
    31441 gtatataaat gttgagactg gcaaagcagg ccagatagcc aattacgcag gcaatccata
    31501 acgctcctac accgaacagt tgtactacaa tttttggaag cgatgctgcc gcggccattt
    31561 gttcaccata ggcgtcgaag tgtaagacga ctaccgtaca gccccagtag actaatcctg
    31621 ccagcagcag accaatcatc aaagcacgag gaaaatcacg ttctggattt ttaaattccg
    31681 aggcaagatg ggcaaatgct tccagaccga caaaacacca gaacatcact gataacgcag
    31741 caaataaccc ggtaagttcg atatttcctg gcgcggggaa ggggatactc gcaggtttga
    31801 tatcgcccgc ccaccagata gcgacaatca gtgcgacgat aagtccggca ataactgttt
    31861 gtagatttgc actggaactg gcaccgcgag taccgatata ccacaccaac gccagcgtac
    31921 cgagttctgc caacaacagt tgccagctat gccagccaaa cattgcctgg ccgaatccgg
    31981 cagcaatttg tagcgcggca ggcaaaccca cgggaatgac cgataaaaac agccagccgg
    32041 tgactcgctc aagccgcgaa ccaaacgcca taccgacgaa gtgcgcgacg ccgcctgcgc
    32101 tgggatagtg gcgacccaga atcgcaaaca caatcgcaat cgggaacact aagataatca
    32161 aaatgggcca cgcccacagg ctgttattgc ccgctaccag tgcagctaac gcaggaacgg
    32221 caaacacgcc agtgcctaat aatgacgtcg atagcaggcc aatgccctgg gccagcccca
    32281 gttcttgttt gagtccactc atgggttgat gtccgattgc gcccaaattt tgggcaactg
    32341 cgtagatttt cgatggtagc acaatcagat tcgcttatga cggcgatgaa gaaattgcga
    32401 tgaaatgtga ggtgaatcag ggttttcacc cgattttgtg ctgatcagaa ttttttttct
    32461 ttttccccct tgaaggggcg aagcctcatc cccatttctc tggtcaccag ccgggaaacc
    32521 acgtaagctc cggcgtcacc cataacagat acggactttc tcaaaggaga gttatcaatg
    32581 aatattcgtc cattgcatga tcgcgtgatc gtcaagcgta aagaagttga aactaaatct
    32641 gctggcggca tcgttctgac cggctctgca gcggctaaat ccacccgcgg cgaagtgctg
    32701 gctgtcggca atggccgtat ccttgaaaat ggcgaagtga agccgctgga tgtgaaagtt
    32761 ggcgacatcg ttattttcaa cgatggctac ggtgtgaaat ccgagaagat cgacaatgaa
    32821 gaagtgttga tcatgtccga aagcgacatt ctggcaattg ttgaagcgta atcctcgcac
    32881 gacactgaac atacgaattt aaggaataaa gataatggca gctaaagacg taaaattcgg
    32941 taacgacgct cgtgtgaaaa tgctgcgcgg cgtaaacgta ctggcagatg cagtgaaagt
    33001 taccctcggt ccgaaaggcc gtaacgtagt tctggataaa tctttcggtg caccgaccat
    33061 caccaaagat ggtgtttccg ttgctcgtga aatcgaactg gaagacaagt tcgaaaacat
    33121 gggtgcgcag atggtgaaag aagttgcctc taaagcgaac gacgctgcag gcgacggtac
    33181 caccactgca accgtactgg ctcaggctat catcactgaa ggtctgaaag ctgttgctgc
    33241 gggcatgaac ccgatggacc tgaaacgtgg tatcgacaaa gctgttaccg ctgcagttga
    33301 agaactgaaa gcgctgtccg taccgtgctc tgactctaaa gcgattgctc aggttggtac
    33361 tatctccgct aactccgacg aaaccgtagg taaactgatc gctgaagcga tggacaaagt
    33421 cggtaaagaa ggcgttatca ccgttgaaga cggtaccggt ctgcaggacg aactggacgt
    33481 ggttgaaggt atgcagttcg accgtggcta cctgtctcct tacttcatca acaagccgga
    33541 aactggcgca gtagaactgg aaagcccgtt catcctgctg gctgacaaga aaatctccaa
    33601 catccgcgaa atgctgccgg ttctggaagc cgttgccaaa gcaggcaaac cgctgctgat
    33661 catcgctgaa gatgtagaag gcgaagcgct ggcaactctg gttgttaaca ccatgcgtgg
    33721 catcgtgaaa gttgctgcag ttaaagctcc gggcttcggc gatcgtcgta aagctatgct
    33781 gcaggatatc gcaaccctga ctggcggtac cgtaatctct gaagagatcg gtatggagct
    33841 ggaaaaagca accctggaag acctgggtca ggctaaacgc gttgtgatca acaaagacac
    33901 caccaccatc atcgatggcg tgggcgaaga agctgcaatc cagggccgtg ttgctcagat
    33961 ccgtcagcag attgaagaag caacttctga ctacgaccgt gaaaaactgc aggagcgcgt
    34021 agcgaaactg gcaggcggcg ttgcagttat caaagtaggt gctgctaccg aagttgaaat
    34081 gaaagagaaa aaagcacgcg ttgaagacgc cctgcacgcg acccgtgctg cggtagaaga
    34141 aggcgtggtt gctggtggtg gtgttgcgct gatccgcgta gcgtctaaac tggctgacct
    34201 gcgtggtcag aacgaagacc agaacgtggg tatcaaagtt gcactgcgtg caatggaagc
    34261 tccgctgcgt cagatcgtcc tgaactgcgg cgaagaaccg tctgttgttg ctaacaccgt
    34321 taaaggcggc gacggcaact acggttacaa cgcagcaacc gaagaatacg gcaacatgat
    34381 cgacatgggt atcctggacc caaccaaagt aacccgttct gctctgcagt acgcggcttc
    34441 tgtggctggc ctgatgatca ccaccgaatg catggttacc gacctgccga aaaacgatgc
    34501 agctgactta ggcgctgctg gcggcatggg tggcatgggt ggcatgggcg gcatgatgta
    34561 attgccctgc acctcgcaga aataaacaaa cccccgggca gaaatgtctg ggggtttttc
    34621 ttttggtcat ctttctagta taagattcag acacggacga cgcgagtggc gtccagctca
    34681 ttgattatgg ggaataacat gcacgtaaaa tacttagcag ggattgtcgg tgccgcgcta
    34741 ctgatggcgg gttgtagctc cagcaacgaa ttgagtgctg ccggtcagag tgtacgcatt
    34801 gtggacgagc agccaggcgc agagtgccag ctgattggta ctgcgacagg taagcaaagc
    34861 aactggcttt ccgggcaaca cggagaagag ggcggttcta tgcgcggcgc agcaaacgat
    34921 ctgcgcaacc aggcggctgc aatgggcggt aacgtgattt atggcatcag tagcccgtcg
    34981 cagggaatgt tgtccagttt tgtcccgacg gatagccaga ttatcggcca ggtatataag
    35041 tgcccgaact gatgtgatgg tgacaaacgt ataattgcca gatgcgcttc gctatcaggc
    35101 ctacataagg cattgcaact gattgaatgc ttgagaattt gtaggccgga taaggcgttt
    35161 acgccgcatc cggcatgacc aacgatcacg ttatcagtca tttcaggctg gatggctcca
    35221 gcctcgcatt agcaaagatg agattatttc gcctgtggtg cagttttgct ggtggattga
    35281 acacgtaagt gatgcagcgc gcgtaagcat tgatcctggg ttagcggttg ggcgttattc
    35341 gccgctacgg tgcgaacata gacctgacat ggcacaccca cgagtttttt cagtcgtggg
    35401 ctgaaatcaa gcattcggcg agtgatcgcc tctatttctt tacccggcgt tgcttcacgc
    35461 gttttcacca ctgcgccgct aaattgttct ttgccacttt catctttaaa gcggtaaagc
    35521 accgccagat aatggtcgaa caagttatgt ttccattccg gttgggaata ggtgtcatat
    35581 tccagattgc cattttgctg gcagtcaacg tcatataacg gcaggaaatc taacggtgag
    35641 gtaatacgta atgccagttc acgcatttcc gcattattaa ttgcatgaat gattgctcgc
    35701 gccagcattt caatctgcaa ctcattgacg actaactcac atttgctacc gtcatgtagc
    35761 gttaatacga aggtaagatt ttcgccttga ttatcggtaa gttccagcgt gttaacccga
    35821 cgattaatat cggcattttt aagctcatca accagaattt ctgggatgtt ttctgccatt
    35881 ttcttaatga ctttatcgcg agcttgctca tactgaaggc gtgcagcagc atccagttta
    35941 tgcttttgat gcagacgact ttccagcgca atcagcaggt cgcgcagttc catgacagac
    36001 ataaagaaca acgattcttt attgcgaggt tctttaattt tcagcgccag tgcaatgaaa
    36061 ttattactct tgcgaataac accggtatta acacccttga tgcttatggc catgtaggtt
    36121 ctccctaacc atttctcaat taaataatta attttaattt ataagccaga taaatgggct
    36181 tggtagtaat agttgttaaa ataacataaa tagccgtact cactctatat aaacagaaaa
    36241 gaaaggttaa ttattggtgt tggttatata aagagtaaca acagcaatgt atatgaataa
    36301 ttaaaggtta tgtaacgggc aaagattaaa cttccctgtt taatcagtta ctgctggcgt
    36361 agctggagat ccagcggcgt tttgctgggt tcgccgccaa tttctcgcgc cagtttcggc
    36421 accagatatc ccgacaccag tgtcagcaac tcacgcataa tctgccgtgc ttcgtcatca
    36481 ctcaccataa aatgcgccgc gccctgtact ttatcgagca catgcaggta atacggcatc
    36541 acgcctgcat cgaacaacgc attactcagg tttgccagcg tttgtgcgtt atcgttcaca
    36601 ccacgtaaca gaacgctctg gttcagcaaa gtaacgcccg cccggcgcaa cttagccatc
    36661 gcctgacgga atgtttcatc tatctcattg gcatggttga tgtgattcac cagcaagatt
    36721 tgcagcgtag aatgtgaaaa gcgttcaacc agcgcatctg tgatgcgtgc cgggatcaca
    36781 atcggcagac gactgtgaat ccgcagacgt ttgatatgcg ggatggcttc cagttgtgtg
    36841 agcaaccagt ccagctcgtg atctttcgcc atcagcggat cgccgccgga gaaaatcatc
    36901 tcgtccagtt ccggatgcgc agcaacatac tcaagtgcag tttgccagtt acgcttgttg
    36961 ccctgatttt cggcataggg gaagtggcga cggaagcaat agcggcaatt taccgcgcag
    37021 ccgcctttga ccagcaaaag cgcccggttg tggtatttat gcaacaaacc aggcactacg
    37081 ctgtgctgtt cttccagcgg gtcggtggag aatccgggcg cgacgacaaa ctcatcttgc
    37141 gaggtaagta cctgacgcaa aagcggatcg tccggattgc ctttctccat gcgatcgata
    37201 aatgagcggg gcacacgcag ggcaaacagc tttttggcgt tgcgtccggc caacagtttt
    37261 tcgtccgcgt ctatattcaa aagacgcaga agttcatcag gatcggtcac aacatcggca
    37321 agttgcgtta accaatcttc tctggatggg gtatttaggg ttacaatatg cgccattttg
    37381 tggcttagct accaattaac aaatttcaga gggccttatg gcaacgtact atagcaacga
    37441 ttttcgtgct ggtcttaaaa tcatgttaga cggcgaacct tacgcggttg aagcgagtga
    37501 attcgtaaaa ccgggtaaag gccaggcatt tgctcgcgtt aaactgcgtc gtctgctgac
    37561 cggtactcgc gtagaaaaaa ccttcaaatc tactgattcc gctgaaggcg ctgatgttgt
    37621 cgatatgaac ctgacttacc tgtacaacga cggtgagttc tggcacttca tgaacaacga
    37681 aactttcgag cagctgtctg ctgacgcaaa agcaattggc gacaacgcta aatggctgct
    37741 ggatcaggca gagtgtatcg taactctgtg gaatggtcag ccgatctccg ttactccgcc
    37801 gaacttcgtt gaactggaaa tcgttgatac cgatccgggc ctgaaaggtg ataccgcagg
    37861 tactggcggc aaaccggcta ccctgtctac tggcgctgtg gttaaagttc cgctgttcgt
    37921 acaaatcggc gaagtcatca aagtggatac ccgctctggt gaatacgtct ctcgcgtgaa
    37981 gtaatgcggt tgtggtgcgg cctgcaggct gcaccatcac ttattcaggt cagagatgat
    38041 gaaacgcctt atcgttcttg ttttgcttgc cagcacgctg ctcacgggct gtaacaccgc
    38101 tcgcggtttc ggcgaagaca tcaaacatct cggcaactcc atctcccgcg ctgccagcta
    38161 atttttcttc tcttccgaaa aatcatcaga tttccatcat ttttggcgat gttgtctatt
    38221 attaatttgc tataggcaaa cataaataac attacctaaa aggaagacgt tatggtgaag
    38281 aagacaattg cagcgatctt ttctgttctg gtgctttcaa cagtattaac tgcctgcaac
    38341 accacgcgtg gcgttggtga agacatttct gatggcggta acgcgatttc tggtgcagca
    38401 acgaaagcgc agcaataagc aataacggta cgacagctgt gtcgtgccgt ttgttttttc
    38461 tgcgatagtc acaaaggtaa tagttgaaat tcccccgcca cctggcaaaa tatccgttca
    38521 accatcagct ttgcaggacg acctgcaaac gcgtcttttc accggggacg gccccaattc
    38581 tccggagcct gatatgtcct ggattatctt agttattgct ggtctgctgg aagtggtatg
    38641 ggccgtaggc ctgaaatata cccacggctt tagtcgtttg acgccgagtg ttattaccgt
    38701 gacggcgatg attgtcagta tggcgctact tgcctgggcg atgaaatcgt taccagtagg
    38761 gacggcttat gccgtgtgga cgggtattgg cgcagtcggc gcggccatca ccggcattgt
    38821 gctgctcggt gagtccgcta acccgatgcg tctggcgagt ctggcgttaa tcgtattggg
    38881 gattattggt ctgaaactca gcactcacta actaccgggc tgctgtaccc aaataaactt
    38941 actgacatca aacccttccc gggtcgcgac tgccagcatc tcctgtttca cttcgtcaga
    39001 aatggttggc gtgcgggaga gtatccacag gtagtcgcgg tccggtccgc aaaccagcgc
    39061 atggcggtat tcccgatcga gtgcaataac gttataaccg ccatagaaag gaccaaagaa
    39121 tgacactttc agcgcagcgc gagttggtgc gccggtaaag tacgctttcc cttcactctg
    39181 ctgccacatt cctctgtcag ggttatagcc tttattaatg acattcaggc cgccgtcatc
    39241 acgcaggctg tatgttgcgg tgactttttc cagtccacgt tcaaagcggt gatcaaaacg
    39301 ggcaatctca taccaggtac cgagatagcg tttagcgtcg aaattattca ctacggtcac
    39361 gccacgcggc ggcgtaggag aactgcaggc aacgaccaga aatgcagctg tcgctgcggc
    39421 aacgagaggg agcaggcgca taaatgtttc cttactggtt ttttctaagt gtagatgaca
    39481 gcaaggaaaa gcgcagaaaa ggtccgaaaa ttcggacccg atgaaatttt actgtagagc
    39541 gttgagaatc tgccaggcgg cggcgactct cgctggattg ggatagtttt tgttagccag
    39601 catcacgata cccagctctt tttctggaat aaacgcgaca tagctaccaa atccgccggt
    39661 cgcccccgtt ttatgcaccc atgatgctcg tactgcagga gttggaggcg taatcgcttt
    39721 tacggggcgt gctgccagtg caattttatt gtcactgccg ttaacgatga tgtcaggatt
    39781 taccggccag tccagcattt cccagcccag accctgatac atatcgccgg tttgccagta
    39841 gcgagattgt gccagttgta tcccttgctg aagtgttttg tcgttgatat cacggggatt
    39901 catattgctt cgtacccagc aggccatatc ttcaatggtc gacttcacac cataagtttc
    39961 agcatctaac gcccctggcg aaacatgaac tgccttgcct tcgcgatatc cccaggcgta
    40021 attcttttct tctgcgggcg gtacattaat ccacgtatgg ttgagtttga gtggctggaa
    40081 gacacgagtt tgcatcgcct gctcaaaact caaaccagac ggcttcacag ccagtgcgcc
    40141 gaacaaaccg atactggagt tggcatacag acgttgtgtt cctggagccc atgcaggctg
    40201 ccagttttga tagaagcgca gcaagtcgct tgaggatttc acttcatccg gcacctgcaa
    40261 tggcaggccg ccagcagtgt aggttgcgag atgtaatagt gtgatcccat tccactgttt
    40321 agcggtaagt tcaggccagt attttgttgc gggatcgctt aacttgattt cccctcgagc
    40381 aatagcgtcg ccaccaagca cgcccgtaaa tgttttgctg accgaaccta actcaaacaa
    40441 cgtttgctgt gtgacgggct gctttttggc gatgtccgca tacccccagg taaagtaata
    40501 aggtttaccc tgataaatta ccgccaccgc cataccgggg atcttttgtt gctctataag
    40561 cggggtaatt gtgcgatgca caatatcgtt gatttgttga ggggcagcaa atgtggagca
    40621 agaggcggta attaataagg cgcagagcgt cgttttgaac atagggtctg gtttccatac
    40681 aaaacggccc gccataggcg ggccggattt acattggcga tgcgttagat tgtaacgaca
    40741 ccaatcagcg tgacaactgt caggatagca gccagaccgt agaaaaccca tttgcccgca
    40801 ggtacgtgga ttttcagatc gtgcatcgcg tggtgcatac ggtgtaaacc acaccacagc
    40861 ggcagaacga tcatcaggaa caggaatacg cgaccaatga agctctgcgc gaacgccaga
    40921 acgcgctcgt agctcagtgc atcacccgga aacagcccca gcggcagcag aatacccacc
    40981 agcaggatca tcaccggcgc aataattgcg ctccacatac caccggcccc gaagaggccc
    41041 cagaataccg gttcgtcaga acgctttgga tttggattaa tcatctcagg ctccttacca
    41101 gtacagggca acaaacagga ttacgatggt ggcaaccaca gttaccgccc agagactttt
    41161 gataattggc tctggtccca ttttttcgtc ttttacaatg atattggccg ctttcggtgc
    41221 cagttcaaac caggttttgg tgtgcagcag agctgccgcc agagtgatca ggttaatgat
    41281 cacaataacc gggttttgta aaaagtcgac gaatcccgcc caggcttccg ggccattttt
    41341 cagggcaaac agcccgaaaa tcagttcaat gctgaaccac acagccggaa ccgccgtgcc
    41401 ttcgcgcagc atgtaaaagc gataaaacgg caattttttc caccaggtgg acgtcattgg
    41461 ccgtacatac ggtttacgtt tagtcgtcat gttgcactcc ttagcgtggt ttcagggtcg
    41521 cgataagaaa gtctttcgaa ctttctactt tgccctgttg aatggccgca gccggatcga
    41581 cgtgtttcgg gcagacttcg gagcagtagc ccacgaaagt acagctccat acgccgttct
    41641 ggctgttcaa ctgcgccata cgctccttct taccgtggtc gcggctatct tcgttataac
    41701 gatgcgccag cgtaatggca gccggaccga tgaactctgg gttcaggcca aactgcgggc
    41761 acgcggcgta gcacagacca cagttgatgc aaccggagaa ctggtgatac ttcgccatct
    41821 gcgccggggt ctggatgtta gtaccctgat ccgcggtgcg ggagttgccg atgatgtacg
    41881 gtttgatcgc ttccagactt tcgatgaagt gggtcatatc gaccaccaga tcgcgttcaa
    41941 tcgggaagtt agctaacgct tcaaccttca taccgtcggt gtaatcacgc aggaaggttt
    42001 tacatgccag ttttggcacg ttgttaacca tcatgccgca ggaaccacaa atcgccatac
    42061 ggcaggacca gcggtagctc aggtccggtg ccaggttgtc tttgatgtag cccagcgcat
    42121 ccagtaatga ggtagttgcg tcataaggca cttcatagaa tgcgctatgc ggtgcggtat
    42181 cgacttccgg gttatagcgc accacctcaa ttttcaggtt tttcatctca gccattcgcc
    42241 ttctccttct tattggctgc ttccgcctta tcggctgcat ccgcttcgcc accgtaaacg
    42301 cgtttagctg gcggtagcgt agtaatcttc acgtcgctgt actccaggcg agtcgtgcca
    42361 tcagcatcgc ggaaggcgag ggtgtgtttg aggaagttga cgtcgtcacg cttggtgcaa
    42421 ccttcgtcca gacgctggtg tgcgccgcgg gactctttac gtgccattgc ggagtgcgcc
    42481 atacattcag caacgttcag accgtggccc agttcaatgg tgtagagcag gtcggtgttg
    42541 aacacgctgg aagtgtcggt gatgcgcacg cgcttgaagc gttcctgcag ctctgccagc
    42601 ttgtcgatgg ttttctgcat cagttccggc gtacggtaga taccgcaacc ttcttccata
    42661 gccaggccca tttcgtcgcg gatcttcgcc cagttttcgc cgccatcctg gttaaccaga
    42721 tctttcagac gttgttcaac gccagctgcc tgcgcttcaa ttgccgcttc gttgccatta
    42781 ccggcagttg ctgcacgctc tgtcgcttgt tcaccggcca gacggccgaa gaccaccagt
    42841 tccgccaggg agttggagcc cagacggttt gcaccgtgca gaccaacaga ggaacattca
    42901 cccacggcga acagaccttt aatgcgggtt tcacagttct gatcggtttc gataccgccc
    42961 atggtgtagt gtgcggtcgg acgtaccgga atcggttctt taaccggatc gacgccaacg
    43021 tacgctttcg ccagttcgca gatgaacggc agacgttcat gcagtttttt ctcgccgagg
    43081 tgacgcaggt cgagataaac cacatcacca cgcggcgtgg agatggtgtt gcctttacgc
    43141 cattcgtgcc agaaggcctg agaaactttg tcgcgtggac ccagttccat atatttgttt
    43201 ttcggctcgc ccagcggagt ttccgggccc atgccgtaat cttgcagata acggtagcca
    43261 tttttgttga ccagaatacc gccttcaccg cggcaacctt cggtcatcag gataccggaa
    43321 cctggcagac cggttggatg atactgaacg aattccatgt cacgcagcgg aacgccgtgg
    43381 cttagcgcca tacccatacc gtcaccggta acgatgccgc cgttggtgtt gtaacgataa
    43441 acgcgacccg caccgccagt agccataacg accgcgttag cacggatctg caccagcgtg
    43501 ccttccatca tgttcattgc taccaggccg cgaacatgac catcatcaac cagaatatcc
    43561 agcacgaaat gttcgtcaaa acgctggatc tgcgggaatt gcagagaggt ctggaacagc
    43621 gtgtgcagca tatggaagcc ggtcttatcg gcggcgaacc aggtacgctc gattttcatg
    43681 ccgccgaagc gacgtacgtt gacgctacca tccgggcgac ggctccatgg gcacccccac
    43741 agttccagtt gggtcatttc ggttgggcag tggtggacga aataatccac gacatcctgc
    43801 tcacacaacc agtcgccacc cgctactgta tcgtgaaagt gatattcgaa gctgtcatga
    43861 tcctgcgcga cagcggcgga gcccccttct gcagcaacgg tatggctacg catcgggtat
    43921 acttttgaga ttagtgcgat ttttgcattt ggatttgcct gcgcggcagc aattgcagca
    43981 cgtaatcccg cgccaccggc gcctacaatg gcaagatcgg cttgaaaggt ttgcacgaca
    44041 ttcctccaga ttgtttttat cccacagccc cgtacttcag ggtaagtgcc tgaaagttac
    44101 ggtctgcgaa cgctattcca ctgctccttt ataggtacaa cagtatagtc tgatggataa
    44161 gtctgaaatt tgacgagatc gattttttta gtgcgcaaga gggctaaatt atcactgaag
    44221 atgattaatt taattactaa accatcagat cgtgcttttt ttagtcactg cccaccgttg
    44281 ttttattcct gcgtaaaatt tgtctcgccg tcgcgttgcg agtagacttc gtgcccttgt
    44341 caaaaactgg agatttaact atgagcgaaa cggcatcctg gcagccgagc gcatccattc
    44401 ctaacttatt aaaacgcgcg gcgattatgg cggagatccg tcgtttcttt gccgatcgtg
    44461 gagtgctgga ggtggagacg ccttgtatga gccaggcgac ggtaaccgat attcatttgg
    44521 tcccgtttga gacacgtttc gttggccccg ggcattcgca ggggatgaat ctctggttaa
    44581 tgaccagccc ggaataccat atgaaacgcc tgctggttgc cggttgcggg ccggtattcc
    44641 agctgtgccg cagtttccgt aatgaagaga tggggcgtta tcacaaccct gagttcacta
    44701 tgcttgagtg gtatcgaccg cactatgata tgtaccggtt gatgaacgag gtggacgatc
    44761 tcttacaaca ggtgctggac tgcccggcag cagaaagcct ttcttatcaa caagctttct
    44821 tgcgttatct ggaaattgac ccgctctctg ccgacaaaac gcaactgcgg gaagtggcag
    44881 cgaaactgga tttgagcaat gttgctgata ccgaagaaga ccgcgacacg ttgctacaat
    44941 tgctgtttac ctttggcgta gaaccaaata ttggtaaaga aaaaccgacc tttgtgtacc
    45001 actttccagc cagccaggca tcactggcgc aaatcagtac cgaagatcat cgggtcgctg
    45061 aacgctttga ggtttattat aaaggtattg agctggcgaa tggtttccat gaattgacgg
    45121 atgcccgtga gcagcaacaa cgctttgaac aagataaccg taagcgcgcg gcgcgcggtt
    45181 tgccgcagca ccccattgat cagaatctga ttgaagcctt gaaagtcggt atgcctgact
    45241 gttccggcgt ggcattaggt gttgatcgtc tggtgatgtt ggcgctgggc gcggagacac
    45301 tggctgaagt catcgccttt agcgttgacc gggcataatt ctgaaaaatt actgaattaa
    45361 cagcgaagaa tggcgtgata agtgccattc tgtagcatat gttttcattt ttccgctaaa
    45421 ggccacaatt taagactgga tatttgatat catccaggta tcaattctgt attggttttt
    45481 acgctgcccg ctcaatgttg ggcagtgcag ttcagtgggc gcgttttata cacgcgctga
    45541 aatgaaggat ggtttcatgc ctcacacgat aaaaaagatg agtctgatag gactcatatt
    45601 gatgatcttt acttccgtat ttggatttgc caatagccca tcggcttatt acttaatggg
    45661 ttatagtgcg attccctttt atatattttc tgcattgtta ttctttattc cattcgcctt
    45721 aatgatggct gaaatgggag ctgcttatcg caaagaagaa ggcggtatct attcctggat
    45781 gaataatagt gtcggaccac gttttgcctt cattggtacg tttatgtggt tttcctctta
    45841 tatcatctgg atggtgagca cctccgcgaa agtttgggta ccgttctcaa cattcctcta
    45901 tggtagcgac atgacccagc actggcgtat tgccggactg gagcctacgc aggtggttgg
    45961 tctgctggca gtggcatgga tgattctggt caccgtcgtt gcttcaaagg ggattaataa
    46021 aattgcccgc attactgcgg tgggcggtat tgcagtaatg tgtctgaatt tagtattgct
    46081 gttagtaagc attactattt tgttattaaa tggtgggcat ttcgcgcagg atattaattt
    46141 ccttgcatca ccgaacccag gttatcagtc cggtctggca atgctatcgt ttgtggtatt
    46201 tgctattttt gcctatggcg gaattgaagc ggttggtggt ctggtcgata aaacggaaaa
    46261 tccagaaaag aactttgcca aaggtattgt ttttgccgct attgttattt caatcggtta
    46321 ttcgctggca atatttttat ggggcgtcag cacaaactgg cagcaggtat taagtaatgg
    46381 ttccgttaac ctcggcaata ttacctatgt gctgatgaag agcctcgggg tgacgctggg
    46441 taacgcactg catttgtcac ctgaagcgtc attgtcgctg ggcgtatggt ttgcgcgtat
    46501 taccggactt tcgatgttcc tcgcttatac cggtgcgttc tttacgcttt gctattcacc
    46561 gctgaaagcc atcatccagg ggacgccgaa agcgttgtgg ccggaaccga tgacgcgcct
    46621 gaatgcgatg gggatgccgt ctatcgctat gtggatgcag tgcgggttgg ttactgtctt
    46681 catcctgctg gtttcgtttg gtggcggtac cgcatcggcg ttctttaaca agctgacgct
    46741 gatggcgaac gtgtctatga cgcttcctta cctgttcctc gcgctggctt tcccattctt
    46801 taaagcacgt caggatctcg acagaccatt tgtgattttc aaaacgcgta tgtcggcaat
    46861 gattgcgacg gtggttgtcg ttctggtggt gacatttgcg aacgtcttca ccattattca
    46921 gcctgtggtt gaagccggag actgggacag cacattgtgg atgattggcg gccctgtctt
    46981 cttctcgctg ttagcgatgg cgatttacca gaactattgc agtcgcatgg cgaataaacc
    47041 tgagttagct ctcgactgat catatgaaat cctgcaactc atcgtctctc cggcgatgag
    47101 ttgtcaggaa gtaatgcgta tggatgatac tctccagggc cggtgataac tgaagatgat
    47161 gttaagctgc taaatgagcc acgttgacat catttggcag cttcatgcat tacaaacttc
    47221 ccgcctgacg acctttgccc gcagacgtca gcgttctccc cgtttgttta ccgttgaggc
    47281 tttccagacg catctggaag ggcgggaatg gcatatcgat accgtgggca tggaagccag
    47341 ccagaatcag ctggtggatc tcatggcgta gcggcatacg gtgacccatc tcagcggcgt
    47401 aaatacgcag ctcgaaaatc tgaatcccct gttgcagatc caccaggaag acttccggtg
    47461 ccgggttgtc gatcaccagc gagcagcgac gcgctgcggt gagcaggatt tccgtcactt
    47521 cttcactgtt ggcatcggca ggggccggaa tcgtcaacac cacgcgcgtg accgagtcag
    47581 agagcgacca gttgataaac tgctcggtaa taaacgcctt gttcggcacg attatctctt
    47641 tacggtccca gtcgctgatg gtggttgcgc gggtattaat tttcgtcacg ctaccagtaa
    47701 gatcgcgaat tgtcaccgta tcgccaatgc gaatcggttt ttcgaacagg atgatcaggc
    47761 cagagataaa gttggcgaaa atttcctgca aaccaaaacc gagaccaaca ccgagcgcgg
    47821 cgaccagcca ctgcaatttc gaccactcaa taccaatcat cgagaagccg accagcccgc
    47881 caatcagcat cagcagatat ttggtgatgg tggtgatggc gtagccggta cccggcgtta
    47941 aatccaggtg ctgcaaaatc gccagttcca gcagcgcggg cagattgcgc accagctgcg
    48001 tggtgatgat aaacaccaga atggcaatca gcaccgcacc gagggtaatc ggctccagac
    48061 tttctacgcc ctgtaccgtg gaggtgacat cccacagcga aatattttcg aggaagccga
    48121 aagcggaatg gatttctgac cacagcacaa tgaccgaaag cagggcgatc aacatcaaaa
    48181 ttgagcgcac tagccgcaag gactgcgcac tgatggcatc gagatcgact tcgctttcat
    48241 cgacttcaat tgccccttcc gggctactgt gatgatgtgc ttcctcttcg ccacgcgcac
    48301 gttgcgctaa catctctgcc cggcgatgct tcgcccggtc aaacgccagc ctgcgtcgtt
    48361 ggatcagcat ccagcggcgg ataacgtgat aaaccaccag tagcaggaac cagatggcaa
    48421 ccgaggtttc aagcctcgct aacagcgcct gtgccgttgc cagataaccc accgccgacg
    48481 ccagaatggc aaccagtggc gcgccaatca tcatgttcca caacatatgg ttggtaatat
    48541 tgtcgccgct gccttctttg ttgagataca gcgggatccc ggcctttttc aggctgaggg
    48601 tgaccaccgc cagcgcaccg caaatgagga taaagcaaag ccgtcccagc gaaccggaaa
    48661 attcgcggtc gtcgaggtta tcgaacatca tcagcgccat aatcagtggc acaataagcc
    48721 cgatgctcat caggtagtag cgcatcccac gagaaacacg ttcgcgcggc cagccaaaat
    48781 gagcgataaa caagccgttc gggcgggcaa aggtagcgca aatcatcact acccacagca
    48841 gcggcacggt ggctgtgacg ccatcaccaa tcgcgaccgc cagcggataa ggccacgcct
    48901 cgcgcaagcc gtaacccagc gtcatccaca gcaccggcag cggtgacgcg acgagaatcg
    48961 accagaaaag agtgcgcaac gtcagccaga agtgatcctg agtcactttg ccgactttcg
    49021 ccgccgaacg ttcaagaaaa cgggtgaaat agcggcgcga gtaaatactg caaccgacca
    49081 gaatcaacgc gccaaacagc ggcaaaatcg tctctttgct ggtcagcatc atcacactgg
    49141 ctttgcccaa ctgactgaag gtgtccagcg aaatgagacg acgcagatcc tgggcgattt
    49201 ccagcggcca ggcgatggtc atcgggcgca cgtcagaggt ccagaacaga tagcggtggg
    49261 tcgcttcgtt cacttctttc agcgcatctt ccagttgccc gttggagact ttcagcttgg
    49321 tcagttccag cagtagcgta tcgccaccct gcaacaatga gttcagcaac tcacgctgag
    49381 tgcgtagctg tgcttccaga atacggtttt gctcagcggt cagcggctga ccgtcggcct
    49441 gatgaatttg ccgtagcagc ggctgtttat taagcagatc ctcataacgt aaccgttgca
    49501 cacgcaactg cgccatttcg gtatcaagct gttgtggttt cggcatttcc ggcagccgtg
    49561 ccacctgcgc ccgcaacgct tcaccgagca gattggacga tcccagccat tgcgactgtt
    49621 cacgcagcgt attcaacgcc tgccggacct gtaacgtctg gctggcagcc tgacgctgtt
    49681 gcgaggcaac gagatccatc cgctgcgcct gttgattcag cgccgccgat agttcgcggt
    49741 taattttgaa ttgcgcgacg atatctttcg gcaaatcggc actgttttct gctagtaatt
    49801 cggtactttc cagcgcccgc tctgcctcaa gctggcgttg gctgttcaat tgattacgca
    49861 aggcctgcaa atacgcatcc agttgctggc tctctttttc agccagctca gagcgcaagc
    49921 gcgctaattc ctggcggtta ttggcagaca gctgcgccag ctccagttca tcaacgagcg
    49981 ccttaagacg tgcagagtca gactgcaacg cgaaattttg tgcctgattg agcggagtat
    50041 tgccggtaag cgtccccagg cggcgctcga tctcatttaa ctgacggcgg gcgtcggttt
    50101 gctgttgcgg cagttgattc agcgaatcgg caatctcgcg ggcgcgctcc tgctcttgct
    50161 gggcctgacg gcttttatcc agcaactgac tgctgacctg gagaatttcc tgattcagcg
    50221 cgtcggtaga catccccggc gacacgctgc gcggctcgtc acgcatgttg tttaattgtg
    50281 cgcgcagagt agcggagagt ttcggataat tatcaatgac ttcttgatat tgcttgatgc
    50341 gctcaaggga accttttcgt tcctcaagcg catttaaggc agactggagt gcctctacga
    50401 cttctggctg cgcgggtttc gccgcttttg cctgctccag ttcctgggtg atttgtttgc
    50461 tatcgggggc cgtcgcggcg tacgcccccc aactgaggca ccaggccatc agaaaagtga
    50521 taatcaggcg cacgtcagcg tttcctttga tggattagac ctggtctttt ttatcgtcaa
    50581 ccaatgggct ggcgtcgtgt tctgcttcga tctcttcagc aggaagcggg gcgggttcag
    50641 cgtctggcgt aacaaaggtt tcggtagata ctgccagcgg ctggccaatt ttcgtgacag
    50701 acaggctttc cagttgctca accagattca ctttacccgg tgcaaacagg ttgataacgg
    50761 tagagccaag tttaaagcga cccatttcct ggcctttcag cagcgccaca gaaccgtcgt
    50821 tttccccggc aggccaggtc cagcgcttga tgatgccttc gcgcggcggc gtaatggtgc
    50881 ccgcccagac cgtctcaatg ctgcctacaa tcgtcgcccc gaccagaatc tgcgccattg
    50941 ggccaaattc ggtatcgaac aggcagatca cgcgttcgtt acgggcaaac agattcggca
    51001 cgttctgagc cgtgagatgg ttaacggaga agagatcgcc cggcacgtag atcatctcac
    51061 gcagaatacc gttgcacggc atgtgtacgc ggtggtagtc acgcggggag aggtaagtgg
    51121 tcacaaacgt accgttgcgg aacaggtccg ccatcagata gttgcctgcc agcagggctt
    51181 cgaggctgta gttgtggcct ttggcttgca ggattttatc ttcttcgatt ttacccaact
    51241 ggctgataac gccatcggca ggcatgacca ggacattcgg atcggtatcg attgggcgta
    51301 cttcgtcacg cagcggacgg acaaagaatt cgttaaaggt gcggtagctg gcggtgtccg
    51361 gcttttgcgc ctctttcatg tcgaccttgt agtatttaac gaacagatcg ataaccagtt
    51421 ttgtcagcca tcctgcccgc ttgcttgcgc cccaacccgc caggcgagta agccatagtt
    51481 tcggcagaat gtactgtagc gaaagtttaa atgaatttaa caaggtagcc tccaggccat
    51541 tgttttgtcg ttcctgatcc ggcctaaatg ccggatcctg aaaaaaaggg gacgattcta
    51601 acgacggtta gcttaattgt cagtcatccg tatcagaaaa gtttttacgc gtttttacct
    51661 gcgccatgct ttccagaata cggtgatagt tttcgaaacg ggtttccgcg atttttcctt
    51721 cctcaaccgc ttcccggata gcgcagcccg gatcggtatc gtgtttgcaa tcgcgatatt
    51781 tacacagacc taaatagtca tggaattcga caaagccctg agtgatttgt tccggctcca
    51841 ggtgccagag gccgaactca cgcacaccag gggaatcaat cacatcaccg ccgtgcggga
    51901 agtgatacag gcgtgcggcg gtcgtggtgt gctgaccaag ccccgagttg tcagagacat
    51961 cgttggtcag gatctctttt tgcagcccca gcaacgcgtt cagcaggctg gatttgccga
    52021 cgccagactg cccggcaaaa atgctgatgc gcccggtcaa cgcctcttcc agcggtttta
    52081 gcccatcctg agtatggctg gaaaccatca atacgcgata accgatattg cggtagatat
    52141 ccatctgttc gttgacgaac gccatgcctt cgtcgtccag caggtctatc ttgttgagca
    52201 caataatcgg ctcaatctgc aaggtttcgc aggccaccag gtaacggtcg ataatattga
    52261 gcgacagctc cggcaaaatg gcggagacaa tgacaatctg gtcgatgttg gcggcaatag
    52321 gtttcacgcc gtcgtagaaa tccggacgcg tcaacactga ggtgcgctca tgcaccgctt
    52381 ccacgatccc tttgacattt acgccttccg ccgccggttt acccggacgc cagactacgc
    52441 ggtcgccggt caccagcgaa cggatggtac ggcgaatatt gcagcggtga acgtcgccat
    52501 cggcggattc cacatcagcg tgcataccaa agcggctgat gacgatacct tcatcaggct
    52561 cgccaaacag attgtcgtcg tagtcgggct tctccttaga cgttttaaga cgacgctggt
    52621 gattggcgtt cacgcggcgc tgctggcctt tggagagttt atttttactc aatcgtactg
    52681 actcctggtc gcccctgatg ggcaaaacat ctatgataca cgcaattgtg gatcaatata
    52741 gtcactgtga atgggtggaa aatagcatga gtgccaatga aaacaacctg atttggatcg
    52801 atcttgagat gaccggtctg gatcccgagc gcgatcgcat tattgagatt gccacgctgg
    52861 tgaccgatgc caatctgaat attctggcag aagggccgac cattgcagta catcagtccg
    52921 atgaacagct ggcgctgatg gatggctgga acgtgcgcac ccataccgcc agcgggctgg
    52981 tggagcgcgt gaaagcgagc acgatgggcg atcgtgaagc tgaactggca acgctcgaat
    53041 ttttaaaaca gtgggtgcct gcgggtaaat cgccgatttg cggtaacagc atcggtcagg
    53101 atcgtcgctt cctgtttaaa tacatgccgg agctggaagc ctacttccac taccgttatc
    53161 tcgatgtcag caccctgaaa gagctggcgc gccgctggaa gccggaaatt cttgatggtt
    53221 ttaccaaaca ggggacgcat caggcgatgg atgatatccg tgaatcggtg gcggagctgg
    53281 cttactaccg cgagcatttt atcaagctgt aaaatttttg ctggtcacgt aagcgccgga
    53341 atccacttgc ggcggcgtga ttttgacgct aaattgaacg tcttgctgat aatttcagca
    53401 cttgagataa aaactcaaaa aaaaattttt aggggggttg cagagggaaa gatttctcgt
    53461 ataatgcgcc tcccgtaacg acgcagaaat gcgaaaatta cgaaagcaaa attaagtagt
    53521 acgcgggaat agctcagt
//
LOCUS       DAZFAB010000023        52657 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZFAB010000023 DAZFAB010000000
VERSION     DAZFAB010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 52657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.023"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            61..558
                     /gene="ftnA"
                     /locus_tag="ACHJWA_001555"
     CDS             61..558
                     /gene="ftnA"
                     /locus_tag="ACHJWA_001555"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFO4308183.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(595..834)
                     /gene="yecH"
                     /locus_tag="ACHJWA_001556"
     CDS             complement(595..834)
                     /gene="yecH"
                     /locus_tag="ACHJWA_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFO4308184.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            1026..2237
                     /gene="tyrP"
                     /locus_tag="ACHJWA_001557"
     CDS             1026..2237
                     /gene="tyrP"
                     /locus_tag="ACHJWA_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFO4308185.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(2299..2964)
                     /gene="yecA"
                     /locus_tag="ACHJWA_001558"
     CDS             complement(2299..2964)
                     /gene="yecA"
                     /locus_tag="ACHJWA_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFO4308186.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPAQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(3321..4322)
                     /locus_tag="ACHJWA_001559"
                     /pseudo
     CDS             complement(3321..4322)
                     /locus_tag="ACHJWA_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(4328..4675)
                     /locus_tag="ACHJWA_001560"
     CDS             complement(4328..4675)
                     /locus_tag="ACHJWA_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2511 domain-containing protein"
                     /protein_id="HFO4308187.1"
                     /translation="MKVKKVQLLVTFLSMFSFSAVAMPFKTIERESFNGVWPFNTDEV
                     QLQCLDGNPYVMNFDDNKLYALTGLARIKGKTFGALPLDNNNPFWLDNDAAPGLKKSL
                     GDVTKAAFDLCDK"
     gene            complement(4705..5355)
                     /locus_tag="ACHJWA_001561"
     CDS             complement(4705..5355)
                     /locus_tag="ACHJWA_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4308188.1"
                     /translation="MAVNSNGSNNRVAGRDFHEKNIQIERYDGSHTVNIAIPSNNDDD
                     DRPLLKAQRKELNSLVAAIAEASNTEAFIIWQKVHAEIGVAGIDDMTVNQYKTAESFL
                     HAMLERCKDHDACKALVSLLLRNSEDCGLRQKLLRYCHINFGTGRLNDLTRSQLQSAL
                     SWLEQQSASSHIESSTLPEVRLRASELIRLYPKEIIFFICVGVLVGGVISRAFFNL"
     gene            complement(5371..5775)
                     /locus_tag="ACHJWA_001562"
     CDS             complement(5371..5775)
                     /locus_tag="ACHJWA_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO4308189.1"
                     /translation="MKNDIGRRLREERERLGLSQVAMSDIGGVKKLTQLRYEKGDSFP
                     DAAYLAALSRFGLDVQYVVLGIHSPETYNDDEQELITRFRAASLDVKNAVIGALKGAI
                     SEKETQPSGRELNISGGNNRIAGRDYNETKGR"
     gene            complement(5865..6002)
                     /locus_tag="ACHJWA_001563"
     CDS             complement(5865..6002)
                     /locus_tag="ACHJWA_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4308190.1"
                     /translation="MRAMLEDERCSLAAISSMTARSSRDSRSASLGPVAVLRGMRDLV
                     S"
     gene            6074..6277
                     /locus_tag="ACHJWA_001564"
     CDS             6074..6277
                     /locus_tag="ACHJWA_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO4308191.1"
                     /translation="MSGITININVNAPYVSLQKYAEITGIPLNTCKKMLADGRIIIRP
                     KRAKMEKPEVNLVAMLKDALANS"
     gene            6299..6649
                     /locus_tag="ACHJWA_001565"
     CDS             6299..6649
                     /locus_tag="ACHJWA_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFO4308192.1"
                     /translation="MKKNANNPYSKFRNGVERHVHHVATSASRSNSRYNLNETHATPD
                     GHAVKQIGEHAWLIEKAGIVVHKCPRNPFTGNRIFALNCGDNHFGQDFTLYEALRTVD
                     RLLRGQSFIKQADL"
     gene            6660..6938
                     /locus_tag="ACHJWA_001566"
     CDS             6660..6938
                     /locus_tag="ACHJWA_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4308193.1"
                     /translation="MTKEHAQGVFIRFIDFRGELLLRASAIDGIVPSEKNAATYVYLN
                     GTRLTVELPYQTVREIISEAEKARQVNGDEPYIEIICMDSEAEIQKAD"
     gene            6950..7192
                     /locus_tag="ACHJWA_001567"
     CDS             6950..7192
                     /locus_tag="ACHJWA_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HFO4308194.1"
                     /translation="MGKEYKTLINKAPERFYFRLSASGAHAERAARDSLTRAIRSLYD
                     VAFYADDLDALNELSELICAAECGEHIEPYKLGNIA"
     gene            7189..7302
                     /locus_tag="ACHJWA_001568"
     CDS             7189..7302
                     /locus_tag="ACHJWA_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4308195.1"
                     /translation="MSIFISWLVLIISVACAIGIMRIIHSVKKIERFFTDK"
     gene            7395..7811
                     /locus_tag="ACHJWA_001569"
     CDS             7395..7811
                     /locus_tag="ACHJWA_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4308196.1"
                     /translation="MNAKEKNIINTLKIVSAEQDKLSRAAQKDNQHMAALYALTIAIA
                     TPEAAKVIEEQSKEIDTLKTQSTVAAMNPSSIGRCIYILGSAMMLQYTIIAELHGKYL
                     ITPYHTKESELLTNLRLIERSQAVFIDDAQRAVFNA"
     gene            7835..8038
                     /locus_tag="ACHJWA_001570"
     CDS             7835..8038
                     /locus_tag="ACHJWA_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4308197.1"
                     /translation="MAIKHFPVVRFTSRGREYEVDERLITTIDKHRSEKDAHHIYLTD
                     GTYFCATNVARVNLIRQVQEPRR"
     gene            8035..8301
                     /locus_tag="ACHJWA_001571"
     CDS             8035..8301
                     /locus_tag="ACHJWA_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFO4308198.1"
                     /translation="MTILDYIATHPGCSGGEIAAALNTPTTAINAELRRLWRGGLVIR
                     TNRSTGGRARKTGGQASYHVNPMPFGCSNPLTHMFNQLLKEARA"
     gene            8298..8597
                     /locus_tag="ACHJWA_001572"
     CDS             8298..8597
                     /locus_tag="ACHJWA_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4308199.1"
                     /translation="MSTINHQKLRELAFALQRMATPQKLLAFRAMLSPSAVLALLDQL
                     EHARTTAPAIRLTLHHEIADFCATLGAPGEPETPEAMQQELLQRIDNVFDFFLNQ"
     gene            8920..9150
                     /locus_tag="ACHJWA_001573"
     CDS             8920..9150
                     /locus_tag="ACHJWA_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HFO4308200.1"
                     /translation="MADRKQHRAIAERRHIQTEINRRLSRASRVAQIMHINMLHERSH
                     ALSNIYSASVFSYLADDLHEFQQLIQQQNKLH"
     gene            9223..9588
                     /locus_tag="ACHJWA_001574"
     CDS             9223..9588
                     /locus_tag="ACHJWA_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4308201.1"
                     /translation="MILANDFLEYLLNTERDLAARVRDRYDMYLKSLPVPQLADGKIV
                     IDGRYMIDSHEGNYRLYRIEGGTPSVIGIYQRPSSAIVDVIADSIRITHRHADTEDTV
                     LEIQRLATACRDTLNGMTK"
     gene            9595..12417
                     /locus_tag="ACHJWA_001575"
     CDS             9595..12417
                     /locus_tag="ACHJWA_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO4308202.1"
                     /translation="MTAEYIRDWQQPRHAVGREGTGIPAPESALSSWLDAYRVENERR
                     QEMADAAFSATPLGNLINKSLDAQEKQDKTITLAGDARKQARGAVDEAMASLRLLPSY
                     LRDPLIRHLSFLRKKQEADRQKGKKSWQAERYARGNLRKIFERLERTDHRWLTQGYRS
                     LAGRERLDDLLYLPQLNKHQIQTLATMTAAMFSSTFEKLCDGFGATDGELTMDVTLKA
                     YQMLARMALHLHAMPPHYDALTTDKDRRNEPDTELLPGAILRLTCAEWWKRKLWLLRC
                     EWREEQLRAACLVSRKTSPYLSQDALSEFRAQREKTRDFLKSFMLENEDGFTIDLETV
                     YYAGVSNPVHRKAEMMATMKGLELLAEARGDKAVFLTVTCPSKYHATTENGHPNPKWN
                     GATMRDSSDYLVNTFFAAVRKKLNRDGLRWYGIRTVEPHHDGTVHWHMMVFAHPEEID
                     TIVSHTRDIAIQEDRHELGDDITPRFKAEYVDGSKGTPTSYIATYIGKNLDSRAVDGI
                     DPKTGKPRVDHETGKSMTESVERAIGWARLHRVRQFQFFGIPSRQVWRELRRLASQMA
                     RNPEGPQRLKDDAMDAVLAAADAGCFTTYIEKQGGVLVPRKDYLIRTAYDLADELNDY
                     GEQSVQIYGIWSPLIGESSRVCTHPDNWKLVRRKPGVEDSARENGFDLQGGPAAPWTR
                     GNNCPRVQETDNNGTEQPEERPAPWPQLPDGVDVNEWMRSLKRHERRALMRSLRDKQA
                     KNSSDEMQSWTQSRKQQRPLPDNHELLAKEWRESAESLGLHIGEQQMQHLLRGGSLYV
                     DGSIIAPQGFEIVRKPDTRPDSRITQLWQRLSRNHGVSSTEIRHNPVASYLAQLGASD
                     PEAAARLASTLQQDQNTMKTPVTVLSDMLRAIRDAEHAQRISETTERASRKADLLRGG
                     LTSGNKKQTETGLTNPVNEQKTRRDI"
     gene            12494..13147
                     /gene="parM"
                     /locus_tag="ACHJWA_001576"
                     /pseudo
     CDS             12494..13147
                     /gene="parM"
                     /locus_tag="ACHJWA_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310661.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM
                     domain-containing protein"
     gene            complement(13277..14014)
                     /locus_tag="ACHJWA_001577"
     CDS             complement(13277..14014)
                     /locus_tag="ACHJWA_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mom"
                     /protein_id="HFO4308203.1"
                     /translation="MGNRKQVTSRIISTPELIRYNDNIVGYGSRELRVETISCWLARL
                     VIVNKHYSHRFVNNSYLHLGIFSERELVGVMQWGYALNPNSGARVVTGTQNREYMELN
                     RLWLHDCMPRNSESRAISYALKLIRQLYPQVQWVQSFADERCGCLGVVYQASNFDYVG
                     SHESIFYELDGEWYHEICRNAIKRGGQRGEHLRANIDRASVHKFRQFRYIRFLNKRAR
                     KRLNTKLFKVQPYPKPQTVKTGLKESE"
     gene            complement(13968..14168)
                     /locus_tag="ACHJWA_001578"
     CDS             complement(13968..14168)
                     /locus_tag="ACHJWA_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Com family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO4308204.1"
                     /translation="MYRNIRCRHCNKLLARASFSYLEVKCPRCKTLNQITSPSATEHP
                     TYTRKSCRGEQKASHIPNHQHA"
     gene            14283..14747
                     /locus_tag="ACHJWA_001579"
     CDS             14283..14747
                     /locus_tag="ACHJWA_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4308205.1"
                     /translation="MIQGGDYLLKENEELGGENDHKMIILLTFYTVKVGSRVYVVCFK
                     STGYYIKVKPSIAGTMLGQSVKTCINALLRARGVSYDIPRSPVKQRCIKRRSLSWMKE
                     PVCPACKTPLQLIYRRSRSQNGKTYKPKNASCFRMLICPACSPRRENTQNVH"
     gene            complement(14786..15031)
                     /locus_tag="ACHJWA_001580"
     CDS             complement(14786..15031)
                     /locus_tag="ACHJWA_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO4308206.1"
                     /translation="MPEIKGTVTEELVKQALYSEEVNRVLKAQVRKDFEAQIDAYVDE
                     VLARMVGRSPAENSTENDPQPVEQPEPVQPGTDGTMM"
     gene            complement(15067..15249)
                     /locus_tag="ACHJWA_001581"
     CDS             complement(15067..15249)
                     /locus_tag="ACHJWA_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO4308207.1"
                     /translation="MTFVHTMLLYFCAVVSALYLVSGGYKVIRNYIRRKIDDAAAEKL
                     SKTAQAPSSPNDPTPL"
     gene            complement(15396..17435)
                     /locus_tag="ACHJWA_001582"
     CDS             complement(15396..17435)
                     /locus_tag="ACHJWA_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO4308208.1"
                     /translation="MTSKWVQLSSMPGNFTVKVSGGTAAFLEAPFPPAETKGGMTFAD
                     CLISFNTRDCLWVRPVSGDPSVEITGAGIGAVIPLSADVAGTAEPSDWDNAETHTRPS
                     GNETASSSPSWYYVVVLAGQSNGMAYGEGLPLPETYDRPEPRIMQLARRSTVTPGGKA
                     CQYNDIILADHCLHDVQDMSGKNHPKADVAKGQYGTVGQGLHIAKKLLPFIPADAGIL
                     LVPCCRGGSAFTAGADGTYSDSTGASEDSARWGVDKPLYKDLISRTKAALAKNPKNRL
                     LAVVWMQGEFDIDAKPTEHSALFLAMVEKFRADLAEQAEQCTGGSAAGVPWICGDTTY
                     FWKQKNEPAYQAIYGGYKNKTDKNIHFVPLMTDENDANVPTNNPAEDPDIESIGYYGS
                     TWRNSAATWTSADRASHFSSWARRGIISDRLATAVLTHAGRTTVKADVPSSETEAPVP
                     SPSETEAVTTTLLSYRVSESEGNLKAQGWEPAGGKAEIISDAGGTGGKAMKLTKETGK
                     SSWYLDHDAGTGAELLKNGGLISCRFKVPGDLVANQYVMGLYWPVSSLPQGVTLTGDA
                     GNNLLASFYIQTDAKDLNVMYHNAKVATNNQKLGTFGAFDNEWHTLAFRFAGNNSLQV
                     IPVIDGQDGAAFTLTQSPVGTFPVDKLRVTDITKNATYPVLIDSIVVEVKKAVTE"
     gene            complement(17535..18095)
                     /locus_tag="ACHJWA_001583"
     CDS             complement(17535..18095)
                     /locus_tag="ACHJWA_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO4308209.1"
                     /translation="MLIGYIRVSTNDQNTGLQRNALECAGCEQIFEDKMSGKTTQRPG
                     LKRALKKLKPGDTLMVWKLDRLGRSMRHLVALTDELQQQGINFRSLTDNIDTSTPMGR
                     FFFHIMGALAEMERELIVERTRAGLAAARERGRTGGRPRRLTQEQYEQVGRLLAGGVS
                     RRQVALIFDIGLSTLYRYYPASVSEK"
     gene            complement(18109..18288)
                     /locus_tag="ACHJWA_001584"
     CDS             complement(18109..18288)
                     /locus_tag="ACHJWA_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001449635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4308210.1"
                     /translation="MSCVPSNGTPLIFLGVASLFAATAAPFDGRAFAALFTVSRKPRF
                     QGCRISALKGLSTSF"
     gene            18317..18520
                     /locus_tag="ACHJWA_001585"
     CDS             18317..18520
                     /locus_tag="ACHJWA_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4308211.1"
                     /translation="MADSRYVQSIRRGSRSTIGMQYNIFEVPDGCVLTGLDVAGDGNA
                     TVTAYYRPVQFLIDGSWKTASSA"
     gene            18600..19121
                     /locus_tag="ACHJWA_001586"
     CDS             18600..19121
                     /locus_tag="ACHJWA_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO4308212.1"
                     /translation="MELINVKRYYPEHKPYGEDVQYFQSEDGRDFYESIPLFTKKYKL
                     CISPVTGIICSVAEDVSALYPAGFTVVEVDELPEGVNIDGNWQFSDGLISKVPVNWKT
                     VAEKRRSSLLQEANETVDDWKTELKLDMISDENKLQLTRWMAYIRQLKEMHFNDIASE
                     GHYQAIPWPEKPE"
     gene            complement(19156..20067)
                     /locus_tag="ACHJWA_001587"
     CDS             complement(19156..20067)
                     /locus_tag="ACHJWA_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4308213.1"
                     /translation="MFHVDNNSGVANMPALAPAQSNTTTWFTEGDGQKGISWIGQDWL
                     NILQAELLNILAEASIQPDKAQLNQLTLSIKAIIAANAFSRKNNLKEIADAGAEAQRL
                     ARGYLGLGALATKNSLGPGDVNALAKDQNLADLENAGTARNNLDVYSKSEGDNRYLRR
                     EQNGADIPDKGAFIDNVGLRETVNKAADALPSGGTAVAANRLATPRNINGVPFDGTLD
                     INITSGMTQSTADGRYVQNVQLGAQSYHSPGGNEMSWNYSAPSGCMLSGINVQETGSR
                     SADNIGGVYYRPVQIYINNAWRTVSSV"
     gene            complement(20067..20627)
                     /locus_tag="ACHJWA_001588"
     CDS             complement(20067..20627)
                     /locus_tag="ACHJWA_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HFO4308214.1"
                     /translation="MAVTLTPHQRALLQLLPDGLAWDKRPSSVLAALCLGLSHSTERV
                     SWTGNQMLAERFPDSSRLLLEDWERYLGLPECDMTGATIQERQRYAGNKYRMKPSLNR
                     EFYIRFAAEFGYEIDIQPSPDSQWVSIVTINSETGYRNMNVLDDILTPLRIYEGGALE
                     CILNRYKPAWQTFIYVYANSHEEETI"
     gene            complement(20618..21700)
                     /locus_tag="ACHJWA_001589"
     CDS             complement(20618..21700)
                     /locus_tag="ACHJWA_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFO4308215.1"
                     /translation="MPYQPLPLAQLITQTQQDISQRLPGSQPGVNETTLNAIAYALAG
                     LSAQEHEHLAWISRQIIPTEADEAELLKHCAFWGVIRKPASRADGPVQLMLTTDAGIT
                     EGVLLQRSDGVVYRITGSATGKAGTLNVNVEAESAGRAGNTPTGTRLSFITPQAGINQ
                     TATVTGTGLTGGADVETVPELLSRLVFRVQNPPSGGTQYDFERWAREVPGVTRAWCKP
                     EWPEAGSVGVTFVQDNNPDIFPGEGDVKRVADYIRSHDDPATGQPVGQPLGPTISVFK
                     LTNKPVAFEIRIVPKTPENQAAVKQALTDLLYNESRPGGLVLPSSFWRAVAGVKGLED
                     FEVRSPLKSVMAGDTELLTVGEITWL"
     gene            complement(21700..22137)
                     /locus_tag="ACHJWA_001590"
     CDS             complement(21700..22137)
                     /locus_tag="ACHJWA_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HFO4308216.1"
                     /translation="MADIAVVWDQGCGSLQLNGADLLTDNSLLTAVIISLFTDRRALD
                     SDEIPDGTRDRRGWWGDSFRERPIGSRLWLLSREKTLSSVVSRAQAYADEALAWLHKS
                     GAATSVVCHAMRVGHDRLSLSVKITLPDGSRHPMIFYADMKGE"
     gene            complement(22130..22744)
                     /locus_tag="ACHJWA_001591"
     CDS             complement(22130..22744)
                     /locus_tag="ACHJWA_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFO4308217.1"
                     /translation="MMNDEVISRLLAPVMRGVRLLFGRGVLTGTTDTLKIQNVQITGM
                     DGETFDDVERPQQYGQISVPLPGAEVFLACAGGQRDQAVVLVVEDRRSRPTGLTAGDT
                     GVYHHEGHRIRLTKNGRIIVTCKTLEIYADEGVQVDTPEAHFTGNITVDKNLHVKGNV
                     SIDGTGRSQGAFTMSEAVIAGITYSGHVHHDNGEGSKTGGPENG"
     gene            complement(22734..23858)
                     /locus_tag="ACHJWA_001592"
     CDS             complement(22734..23858)
                     /locus_tag="ACHJWA_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HFO4308218.1"
                     /translation="MSSRVELYLGGEIFSGWLTVSVRRSLEHLAGSFELGVMMPGVRL
                     PSSVRAGQSLELRIDGQPVITGWLDQVRQRISATRFQITLSGRDKTGDLVDCSAIHPG
                     SQWRNRTLEHIASDLCAPFGVTVRWQVNDATAARPFSTFTLENSETVADALTRAARHR
                     GVLVTSNAAGELVFTQAGSQRGDTLTLGENLLDLDHNVDHRLRHSEYRVRGHGRGGGH
                     AGDALTAGTLAAPVGTVTDSAIHRYRPKIVLADHAVDADGARQRAVREMRRAVARSVR
                     LTATVRHWFRENGQLWDINLLTAVTAPRTGVEERDLLVCQVEFSLDANHGETTRLILA
                     PRDGFIVPAEPGNSGSGNAGDVDAFVRAQMKKQGIKFNDE"
     gene            complement(23842..25191)
                     /locus_tag="ACHJWA_001593"
     CDS             complement(23842..25191)
                     /locus_tag="ACHJWA_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HFO4308219.1"
                     /translation="MASGDGRGAFRGVPFLVWREQRERGGRNIVRREYPLRETGGADD
                     LGPKLAEFTFSVLVMGDDVQTQRNRLRDALRAPGAGELLHPDYGTLNVLINSFESRYN
                     AAEQGVVEFTINVTPVSDDTAPAVTQDTAAILEQKSTTALGQVFETLEAGWTVISDGM
                     HDVQAMTETISDKVSALENAVSGMGIVQDISAFTATFTALKGNATALLTAPSRMASSF
                     AGLFSALITLPSLPSLSSGGLNTRPGGSIGRTTSAVSQGMPQLYRTLSSLRYVLDEQD
                     NPQTLIGLTPAAQKNIRLIRAVMQSAAVVAQAQTVGKLLDQVLSQETLPDSDAAHRTW
                     PVWLESSVDLQRINRDLSEALERQVMTLSGQGYTATALTLRDARLALTEDLNTRGVQL
                     PGATVVTVRTTEPALVTLYRATGNSTGWQRFVRRNGIVDPLFIPGGHSVEVISEQQG"
     gene            complement(25178..27253)
                     /locus_tag="ACHJWA_001594"
     CDS             complement(25178..27253)
                     /locus_tag="ACHJWA_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFO4308220.1"
                     /translation="MARNLRASLIVDLLGNISAKSRQWSQELGAFSRSGQAGLGGLGN
                     AARRAGQETDVLGSRMQRTLAGVRGSIRHVTSDFDRLQGSITGTIGRISNLYGMLAGG
                     AAVYGFNRGFIRPAAEMETYMIRLNSLYKGDRAKTDDVRRWAIQNAKETTWGLAGVMQ
                     EYTSSLGFGMSDREARNFVTMLQDQGAVGGWSLSDAQGASLQLKQMYARGSIQAADAN
                     ILATYGINAYRVLADRLGVDQKVVRKLGEKGLLGPDSIRLLFQTLAEQARGAQKDAMN
                     SWSGLTAMMGDVWDQFARDVMDSGPFEKLKGNLRGFLSWVDSAKSDGSYHSLAESTAS
                     AMNQGFEYARDAVTGFYQAIRKVRDTLQALRDAGYGDALDRIGQGAQTAAKYLLYMYL
                     ATRALKMARAVGTGIIRPGAALLGYGLSAAAFLTSPFRRSPPAGTPPGTSQGRGQRFI
                     NFLTGVNPAAVQPVLVTNWPTGGLSGTGATDTSSGGRQGRGRRKRGPGRGRPVTPPLP
                     PSPVPPSPSSGGAGFWERMMGRAGGLLSSVGSRMGLGRFSGFFRSAGGLAGRLGGGAL
                     WAGAMAAPVLLDGSASATDKGEAVGSLAGSIAGGALGAAAGPVGIAIGSTVGSYLGNY
                     LGGWLTEAWQKLRGSDDESSGQAVQKASARVELVAPEGWQARSIDIDDTSGHGLDVNV
                     WNGGNYGLW"
     gene            complement(27380..27856)
                     /locus_tag="ACHJWA_001595"
     CDS             complement(27380..27856)
                     /locus_tag="ACHJWA_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFO4308221.1"
                     /translation="MTTRKKKTAVSEAAVMEAIREALEGADPRTAGLTEQLAKGYVDL
                     LDGLPFGETREYRVTFRELTAKDSIDAEAEAERVVETNNGPMLIASPSLRGVALLRRQ
                     IAAVGDIEGPLSPRQIGQLSERDLSRLMAAVSLLDTALAGKLAADRGRSGAVSGSD"
     gene            complement(27871..28236)
                     /locus_tag="ACHJWA_001596"
     CDS             complement(27871..28236)
                     /locus_tag="ACHJWA_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFO4308222.1"
                     /translation="MSGKQYQGTATIRVNGQEYETLEGATFSPSGFEREVVKGAKVYG
                     YRQKPREATLDCKFPAGGEGSPAADEINNWTAVTIEFVADTGEVHMMTKAWSSEPASL
                     DGGGEISVKFASASSTRVQ"
     gene            complement(28245..29747)
                     /locus_tag="ACHJWA_001597"
     CDS             complement(28245..29747)
                     /locus_tag="ACHJWA_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFO4308223.1"
                     /translation="MINFDGIGNDRRIPLIEVEFNNSMAVTGTPAQRQCVLLFGQARM
                     KENAVDGAGVLDVPVRITRASQATELWGRGSMIALMVAEFIAINPDAELYAIAQGNGT
                     GQATAAAMNLTGTVTRDGIVYACVGGRRYTLPAPKGKKGKELTDELAALINADPDAPF
                     TASSGAGSGDNGAGLKGSLGITARFTGECSVHDVRLNYHDGEATPEGIQVAIAYPKQK
                     AANPDITRSVAGMGDRQYNYVVMPYKDDANLKIISDELLKRWGPAKMSDGVLWLAHTG
                     TFGEVQAFGAKRNDFLATCTSIPKAPEPDYLWAAAVCATCAPSLGADPARPLQTLALP
                     SRMAPAPADRLTREERNSLLWGGIATVSVAAGDVVQIERQVTMYRQNVYGESDPSYLD
                     VETIYTLSWLRYSLRTFITQRFPRHKLADDGTPVRAGQNIVTPEIMKLQLISLGEEWV
                     ELGLVENLDTFKKNLLVERNASDRNRLDVLCTPDLVNQFRFLAAQIRFIL"
     gene            complement(29744..29989)
                     /locus_tag="ACHJWA_001598"
     CDS             complement(29744..29989)
                     /locus_tag="ACHJWA_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HFO4308224.1"
                     /translation="MKTIFIKPAPGRLIRDPDTMRPLAQEGEEKPFTPFWCRRLDDGD
                     VIQAEKAAEEAPAVSADATTATEKPVAQPASDKEKPQ"
     gene            complement(29990..30550)
                     /locus_tag="ACHJWA_001599"
     CDS             complement(29990..30550)
                     /locus_tag="ACHJWA_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HFO4308225.1"
                     /translation="MITETEQAYIARIREYFGNELVSVDTHPGDWSDSVLRTMLINAP
                     AIYVAWLGAGEGRTRGRLVSHWVFYVIGDMLNGREASRPGLYQIVARLIAVLNGFRTE
                     KTSPLYFEKAVNGYTETQADSGAVMYALYFSCEEMIAPLTDISSLDDFLRHYETFVEP
                     PGTPPFEAHIDLPGNTTTAVEPPEEL"
     gene            complement(30547..30966)
                     /locus_tag="ACHJWA_001600"
     CDS             complement(30547..30966)
                     /locus_tag="ACHJWA_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp436 family protein"
                     /protein_id="HFO4308226.1"
                     /translation="MNYATETDMRARYREDLLRPLLAVPRSDEPDTRKLNRALTDASA
                     LIDSYLSARYTLPLEVIPAVLVQHCCAIAFYYLCDQRASDQARDRYREALAWLKDVMN
                     GNVPVGVDTNGAAPESGDLPQVQSDAAVFGRNQKGFI"
     gene            complement(30963..31337)
                     /locus_tag="ACHJWA_001601"
     CDS             complement(30963..31337)
                     /locus_tag="ACHJWA_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1506-related protein"
                     /protein_id="HFO4308227.1"
                     /translation="MNEHHTVVATDDSGLQVSGDNSVTVLAEVRCSRSAFRRAGFLFT
                     RGRQQVEVTPEQLARLEEEPCLTVRILQTSADDAGSVAGVVHAVAGTDLAEAEAEAEA
                     EAEAGQDAPRKKTGNKAEQARA"
     gene            complement(31381..32328)
                     /locus_tag="ACHJWA_001602"
     CDS             complement(31381..32328)
                     /locus_tag="ACHJWA_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HFO4308228.1"
                     /translation="MPTPITPAMITALMTGYRSDFQAGMSMAPSQYKKIAMTVPSTSK
                     SNTYGWLGQFPQFREWIGSRVIEKMKAYGYAIVNKTFEGTVAISRDDFEDDNLGIYSP
                     LFQEMGRAAAVQPDELVFAALRDGISAACYDGQNFFDTEHPVYPKVDGSGDAQMVSNM
                     FVAKTGSVGAQADYSGPAWYLLDCSRAVKPLIYQDRRKAELVAQTKVDEGRAFTDNEF
                     VFGASARRNVGYGFWQMAYMMQSPLTLDALWHGWSAMREFTADGGRKLGIKPTHIVVP
                     TSLEKQAVQLLERELFADGNATVSNEMKGKLELVVADYL"
     gene            complement(32328..33452)
                     /locus_tag="ACHJWA_001603"
     CDS             complement(32328..33452)
                     /locus_tag="ACHJWA_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HFO4308229.1"
                     /translation="MKTKNTPKLAYAILNAISLSADGDGDWCQIMPAGRVKARDGRPE
                     KPAEGWLINRATVERMVSRVVALNQPVKIDYNHQTLIKGHQAPAAGFVMASPENFRFS
                     EERGFEVRPKWNPPALEHLRNNEFPWFSPVIGYDENTGEPVELRMLAITGDPGLTGMN
                     PVAALSADDLYNALNPPLKDTSMNEQLRQLLTALGLTVADGDEFTPELGTAALSALTG
                     IKTRADAHDNLKTQVASLSAELETAKGTPAGGIIDLTKYVPVETYNALRTEYVALSAQ
                     HGSTTLEQVLDKAESEGRIFKSERGYLEQLGGQIGVAALSAQLDARQPVAALTSLQTD
                     TVTVPDKKTATAALSAEDIAAAKLLGKTEAEFLKMKEEMQ"
     gene            complement(33629..34102)
                     /locus_tag="ACHJWA_001604"
     CDS             complement(33629..34102)
                     /locus_tag="ACHJWA_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFO4308230.1"
                     /translation="MSSIDAAVVVDVTRLQRVFARLQFVGGGKDLARSVAASLLSSSE
                     MAFEQEKEPDGERWHDWSDPYRKWRTRKGYMPGKILTLNGDLARRLTTDYGDTWALIG
                     SNEPYAAIHQWGGLPGMPPGPAAIGARPYMGFDQVAEQEIMDEIRKRFKKATETP"
     gene            complement(34224..35555)
                     /locus_tag="ACHJWA_001605"
     CDS             complement(34224..35555)
                     /locus_tag="ACHJWA_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HFO4308231.1"
                     /translation="MAQGIDLGYAATLASKEAVAYFRAKGAHISWNWFETDADVHARS
                     FTAAKVARLDVLTTLQAEVQRAVDEGISQKAFIRTLTPRLQKLGWWGKQIVVDSAGNA
                     EEVQLGSPRRLALIYNVNTRVAYNAGRYTQMMNNTDTHPFWQYVAVMDSRTRPAHSAL
                     NGLVFRYDDPFWNTHYPPNGWNCRCRVRPLSQARLDAMGLSVSSGQDHLSTRNVEAGV
                     DKQTGEVREMPVTTYSDGTRTMTPDVGWSYNPGSAAFGTDQALIRKLIEVKSPALREM
                     VVQEMNNSPERQLAFRIWAKNIMKTRRGGNDIRTLGFMTESIAQAVESRTGTPPARLL
                     AMSGKNVLHADSMKHQNDGIALTPEDFGRLPAMLAKPKAVLWDKRHNNLMYIVESKDS
                     SVQIAVNAPYSLKRQPDKLDVIVNAYRVINMDKLKSDIRGGMLEVLEGDID"
     gene            complement(35539..37128)
                     /locus_tag="ACHJWA_001606"
     CDS             complement(35539..37128)
                     /locus_tag="ACHJWA_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HFO4308232.1"
                     /translation="MGRIIDLDGKPFSFDPEMQSAVLDIPQIASRYIEHPASGITPNR
                     AAQCLRGAERGDLIAQSDLAADIEEKDTHLFAELGKRRLAIQGVPWSIEPPPNASANE
                     KKDAEMLDEYLHSADWFDAMLFDATDAILKGYSCMEIEHGMLGKMHIIRAIRWRDSGN
                     FCLNPDDLSELRLRDGSHAGVAFQPFGWIVHQSRSRTGYGGATGLVRTLIWPFIFKNY
                     SVRDLAEFLEVYGLPMKVGKYPSGATPEQKSALMRAVMDIGRRTGGIIPAGMSLEFQA
                     AANGQADPFETMISWGERSISKAILGGTLTTEAGDKGARSLGEVHNEVRREIRDSDLR
                     QLAATLNRDLVYPLYALNTAHAIDIRRLPRICFQTKEPGDITKITSAVMQLSTGMDIP
                     DPWVREQTGIPQPAPGEAIFRVRQSGNEPAQTDKEMPPEKQEKTEQTALAARLPEAKS
                     SPRDELDDMGDAVPARRLQEAIDPVLEPVIDAIRTRGLAEALADLPALYREMDDSRLM
                     TLLSDAMFAAEMKGMLDGTRD"
     gene            complement(37128..38792)
                     /locus_tag="ACHJWA_001607"
     CDS             complement(37128..38792)
                     /locus_tag="ACHJWA_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4308233.1"
                     /translation="MNNAEELSPLLTNTVSTRKIDLAGEKALLGVDVPDSLDLPGDMP
                     VFLDYQARWFEDESEVCIAEKSRRTGLTWAEAGRNVITAAKPKRRGGRNVFYVGSKQE
                     MALEYISACALFSRAFNQLADTDVYEQTFWDRDKKEEILTYMIRFPNSGFKIQALSSR
                     PSNLRGLQGDVVIDEAAFHESLDELLKAAMALTMWGARVRIISTHNGVDNLFNQYIQE
                     AREGRKDYSVHRITLDDAIADGLYRRICYVTGREWSPESEQKWRDDLYKNAPTREDAD
                     EEYGCIPKKSGGAYIPHALIEMAMIRDIPILTFEAPDDFISRAAWLRESEVLTWCEEH
                     LKPLLEALNPRSRFSFGEDFARTGDLSCFVLLEITESLAKREVFRVELRNLPYAQQEQ
                     VMMYILTRVPALVGAAFDATGNGGYLAEAALLAFGPDIIDCVMLSPKWYGEWMPKLKA
                     EFEDQNILVARHQTTLDDLRHVKVVNGIPQIDKGRTKDQNATAVNARRHGDFAVALCM
                     ANRASYMEGFILDESACQALPERSRAMEGGYRDDDEAYHEFDRGCW"
     gene            complement(38792..39373)
                     /locus_tag="ACHJWA_001608"
     CDS             complement(38792..39373)
                     /locus_tag="ACHJWA_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein Gp27 family protein"
                     /protein_id="HFO4308234.1"
                     /translation="MDKPTRGRVRKVDLLPDSIRKPLLEMLREKRLTQVQIREEINRL
                     IREAGLPEEQQLSPAAISREASRNELIARNLRDLREQTKAMMAELGDKPTGETTALIL
                     EMSRALMYRRLRAATESLNSDSDVDMRLIKDILLSAQRAESAAERSIKREKEIRAAFA
                     EEMANAVTDELRGVDGMSEQLESRIKGILLGKV"
     gene            complement(39376..39666)
                     /locus_tag="ACHJWA_001609"
     CDS             complement(39376..39666)
                     /locus_tag="ACHJWA_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFO4308235.1"
                     /translation="MSDFITEDQRLVILRSLADYNGELGESVLQDCLDDYGHRVSRDT
                     VHTHIAWLAEQGLVRKRVLINGYFIAELTGRGQDVAEGRASVPGVKKPRARG"
     gene            complement(39659..39970)
                     /locus_tag="ACHJWA_001610"
     CDS             complement(39659..39970)
                     /locus_tag="ACHJWA_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2730 family protein"
                     /protein_id="HFO4308236.1"
                     /translation="MWVSIVKDYVVPILSATATAGGIFMALMRKTFVPREAFEKLSDR
                     VEKVETRLSSLPTEAEVNRLNVEIVTLRGELKTTNATLRSVSYQNELLLEQAVRKKTN
                     E"
     gene            complement(39951..40178)
                     /locus_tag="ACHJWA_001611"
     CDS             complement(39951..40178)
                     /locus_tag="ACHJWA_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO4308237.1"
                     /translation="MDDSDRAQAVMERGSERALCNRLTRKRRAMDAPGRRVCADCGGE
                     IPAARLVAVPDAIRCVNCQNIMEARHVGQHR"
     gene            complement(40189..40407)
                     /locus_tag="ACHJWA_001612"
     CDS             complement(40189..40407)
                     /locus_tag="ACHJWA_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4308238.1"
                     /translation="MPIPAEWLADCLVPPVPEPFTFGASVTYNLQLLAVIKNCNVDKA
                     SIRRLEARRQHEFTDMAGTPAVPAGKTK"
     gene            complement(40391..40819)
                     /locus_tag="ACHJWA_001613"
     CDS             complement(40391..40819)
                     /locus_tag="ACHJWA_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4308239.1"
                     /translation="MCGTAVTGCPVAAVNNALPALIAGACMAALGIIAVLVAAIWGMH
                     QKTQRLEDNNQVLVRERDEARQVLANQQHTLQLISQISEAATNEKQQNIQHSEGQQSV
                     VRRSLAAVPAASAPVPDDVADRVRRAVCEIRACEAGADPR"
     gene            complement(40854..41354)
                     /locus_tag="ACHJWA_001614"
     CDS             complement(40854..41354)
                     /locus_tag="ACHJWA_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO4308240.1"
                     /translation="MPKLPAPLRKKLIALVLAGAGTFTIATHYTGYWEGKENSTYIDP
                     TGTPTICYGHTGPDVKPGMTLTDEECLELLEKDMKWAFAAIDRRVQVPLTRGQTVALA
                     SWIFWAGETNFRNSTLLRLINAGQMPASCKQYIRWIYSKGVKLPGLEARRSADEWLCR
                     YDLPKV"
     gene            complement(41426..41851)
                     /locus_tag="ACHJWA_001615"
     CDS             complement(41426..41851)
                     /locus_tag="ACHJWA_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFO4308241.1"
                     /translation="MAETQMSMFGGDSEQLHALIDRLDDIPDDVLKKNWPRTLSELVE
                     VTGAELQRQGIEPVLAGKLARKVAAAQAAYMGGRGYYLPVGESLFAELRNNEIFSRWD
                     RGEKIESLRRHYRMSETQIYTVIREQRRLHLARTQPPLF"
     gene            complement(41921..42433)
                     /locus_tag="ACHJWA_001616"
     CDS             complement(41921..42433)
                     /locus_tag="ACHJWA_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HFO4308242.1"
                     /translation="MNRASLITLIHVAKRDLQLDRETYTSALLAATGKTSCRDMSPDE
                     LSRVLDVLKKRGFKVRQKPVNRALKPGTVTAKIRAIWKVMHRQGFITDGAETALNRWV
                     KSQTAAQNGGEGVANWQWLEQHPALASDVLERLKRWHRRKMLAAMGMPERTLMGYDAV
                     CRQYEKSLPR"
     gene            42504..42743
                     /locus_tag="ACHJWA_001617"
     CDS             42504..42743
                     /locus_tag="ACHJWA_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4308243.1"
                     /translation="MSDIKNALDVAAFTMAGNLMVTLFDKGVISREEATHIINIAQKS
                     AAPQSAEGHQDGSYINTNWHFDNLRACMGITAKKD"
     gene            complement(42724..43011)
                     /locus_tag="ACHJWA_001618"
     CDS             complement(42724..43011)
                     /locus_tag="ACHJWA_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4308244.1"
                     /translation="MEKEIKFAPIDIDEELAKIGMPERMRDIIEYAIKENLGAREALS
                     IMEREINLINEAVSLENKIAREEYVRRRLGVDGSAIITSEHYAKIFNLFSR"
     gene            complement(43149..43481)
                     /locus_tag="ACHJWA_001619"
     CDS             complement(43149..43481)
                     /locus_tag="ACHJWA_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4308245.1"
                     /translation="MITTLFVESDEPLVCAAGMPVCGGTLTGVYFGDLRGYPWHSLDD
                     AFPPDMEAVVLIVQYGDQQELRIGHMGYEGFFIDEETGACLEDEDGQVTHWCHISSLP
                     ELQETPNG"
     gene            complement(43478..44029)
                     /locus_tag="ACHJWA_001620"
     CDS             complement(43478..44029)
                     /locus_tag="ACHJWA_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family protein"
                     /protein_id="HFO4308246.1"
                     /translation="MMILQPMGRKGRAPAHVRAWTPEEDALLIALYPSTPVKDIAVRV
                     KRSYWGVYNRIVLLRGTYPESLKCKRPRFKHDEDKFIRKNARTMTGKQIGEYLGRDRD
                     SVHNRARYIGVSMKKYGELLPFTRIPDDDVHLIRELRDAESPRRLTFREIGEKFELSE
                     GTVSFIYHRRRTAEDVVLRELTP"
     gene            complement(44033..44548)
                     /locus_tag="ACHJWA_001621"
     CDS             complement(44033..44548)
                     /locus_tag="ACHJWA_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4308247.1"
                     /translation="MFFKTSNPSALAAWQKYQQDCQKVKDEAKRLEAVLNVACRSVFV
                     SGISGFCFKGLRFMDDKYPFHRDLWRKPTASNGWSCTPRTSRIPKALRVASDELNSLW
                     REYSPVTYARTDALLFWLGIDFSAILHGPVKWFCVDDVIYLQCGVKPAKQRMTEILPD
                     EFYAAEKRVRG"
     gene            complement(44548..45081)
                     /locus_tag="ACHJWA_001622"
     CDS             complement(44548..45081)
                     /locus_tag="ACHJWA_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4308248.1"
                     /translation="MIDAKVLEGVKNWLRIYGRLTCGVLAEKMNMPPSSMVYFLRDAV
                     DAGVLTECNGFYDIPRPRPVQPVRRKCSQESAADDVQWCSFRKSLPWIEGHDIPSMAW
                     EFAQGILTCETVYVVAEVDEQAMKEGVPQFVMAYIDIRLGVIICGLSGWNITSRVLRY
                     LIVDRTAAPAGISAEVA"
     gene            complement(45085..45663)
                     /locus_tag="ACHJWA_001623"
     CDS             complement(45085..45663)
                     /locus_tag="ACHJWA_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5420 family protein"
                     /protein_id="HFO4308249.1"
                     /translation="MSIKYRYFKMNEHDSRHYHREWLDSVGRERQKLIDDFLSNQNAR
                     GYSYSWCCGDFFVKSILVHEDVDVGRNKRVLSDKFDEDGRTLFSVTPDRRFREGKKLN
                     KALNILNEKLKQLPAFSTWMVNKLDCYFEVFGISNGRTVMACSSAGFYGDKGAVVVRI
                     PVGESDNAFSPEKLHPSLMAIKHSEFIAITEE"
     gene            complement(45748..46278)
                     /locus_tag="ACHJWA_001624"
     CDS             complement(45748..46278)
                     /locus_tag="ACHJWA_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO4308250.1"
                     /translation="MAKRVTKLKAAAEAAPQTREEVSRDIRTLGDIQREALRLETAMN
                     DEVAEITARYTPQIENLKKEIKVLFKGIQDWCKTNRDELTNGGKTKTANLTTGTVSWR
                     LGTPSCSVSRDVEGVIEMLRRMGLERFIRTKEEVNKEAVLAEPDAVKGIAGIKVNKGA
                     ESFYVEPFEQDAGLNK"
     gene            complement(46587..46859)
                     /locus_tag="ACHJWA_001625"
     CDS             complement(46587..46859)
                     /locus_tag="ACHJWA_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4308251.1"
                     /translation="MSKVVRIIFEYKEHVIHKNADGTVRMGVSLDIRSTGIKQKGDGP
                     AMIFGVVMLAESRDFAELVAMKARALMKDMNMSSGVIKGNEFNQQG"
     gene            complement(46856..47137)
                     /locus_tag="ACHJWA_001626"
     CDS             complement(46856..47137)
                     /locus_tag="ACHJWA_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host nuclease inhibitor protein"
                     /protein_id="HFO4308252.1"
                     /translation="MKKNLIAWAWSSGLIEFGYVLPEGALPIVAGKPATVRHVIEVMA
                     RHGRDEQEQLLVPGIPEAVTEEEAFNAMIRFCREVRRRVSYPNRTRTRG"
     gene            complement(47139..47393)
                     /locus_tag="ACHJWA_001627"
     CDS             complement(47139..47393)
                     /locus_tag="ACHJWA_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4308253.1"
                     /translation="MNMQSCGNKMNLFDSLNSARRLTELAGAVLERSKRYPQRFALKT
                     TPPVGNVQGTGEIEITIQTNGLRRRVKATRISGCTVYWEV"
     gene            complement(47406..47627)
                     /locus_tag="ACHJWA_001628"
     CDS             complement(47406..47627)
                     /locus_tag="ACHJWA_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4308254.1"
                     /translation="MAVFNIPDIYGRFYLVNFDNVKVISLAENKECGDLLFEFNDRTR
                     MVISAGLDREGATEVYSGICRSVGAKQVS"
     gene            complement(47630..48562)
                     /locus_tag="ACHJWA_001629"
     CDS             complement(47630..48562)
                     /locus_tag="ACHJWA_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO4308255.1"
                     /translation="MTDINDVIKTIDELIDGGVLTQYAIAREAGISDGTLSAFRKGKY
                     KGDNAAVAASLRSWYENWNKQSALPEPPQFVETQTVQELRALFQAVRLMGCINVIVGV
                     PGVGKTATARNYCQEQPNTWMITLSPAHSSVTECLLELAEELGIDYTRANKGALSRAI
                     RRRLMGTRGLVIVDEADHLGIDGLEQLRAIQDATGIGMVLIGNPRGLFKGGRRAFDDL
                     SRLFSRLARTKQLRKAKKADVLAIARAWGISGEVELAVMQAIAEKPGALRVLTHTLNQ
                     AWLTASGEGAALTEKHINAAFKEVYTNPELLSQV"
     gene            complement(48634..50724)
                     /locus_tag="ACHJWA_001630"
     CDS             complement(48634..50724)
                     /locus_tag="ACHJWA_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HFO4308256.1"
                     /translation="MYVIAKELIGAPGMPATTKGIRQALQRYVQGKSCCSRRRSGSKA
                     TEYSIDCLPEVTQQALRERYALQLMTQKADESPAPVVTKARRSPAVVDAVEAYRGSPQ
                     LMVERLNALTENQRQVADARIAIVSEVMKVAQQPGFSCAKAIRFIVDNLARSQLDERI
                     VAMVETANAKKGNSRALSEITLKRWIAAFNKAQNAAERLLLLAPGKRQEIKAEDISWL
                     PEFLAQYRQSNGRPMTEAYEDFVAEWQHRHADEPYMLDIMPSYDTIRRAMKKLPEVVK
                     QKGRVTGSEYRQLEGFTRRDWSKMPVNYVWIGDGHGMKLKCAHPVHGRPFAPEVTFVI
                     DGGTRFVVGWSLDLAENVFAVAGAIQHGIRHHGKPFLYYSDNGSGETADILDKEVVGI
                     LPRLGINHPTGIAGNPQGRGIIERLNRTLPMRIARKYRTYFGKGADRETLRKTNRDLR
                     SAFTALQQGKRLNARQQSVMRDLPSWSELIDAIRDGVEWYNNRPHDELPMKPNGKHYS
                     PAEFRKKRLAEEDTEIEWLSDVELRDMFRPMVERPVRRCEIRWLNNIYYAPELRDEHG
                     RKVLISYDIHDAERITVRRPDGSVICEAVWDGNKREAFPVSAEYYKQQQRLKGMRKRA
                     EEKIRDAEDEVVNVLEHKPQEPWLENIYRPVGNTVTVQQPVADDEPDEEYERNFQRGL
                     QLLEAKLKENDPLA"
     gene            complement(50726..50974)
                     /locus_tag="ACHJWA_001631"
     CDS             complement(50726..50974)
                     /locus_tag="ACHJWA_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO4308257.1"
                     /translation="MSRREVSDHDWPPEIIKARLHMAGLSLRSLSLKAGYSRDSLKSV
                     LRTPCRPYQQIIADALGVSPEEIWPSRYQVKSYMRKAS"
     gene            51142..51726
                     /locus_tag="ACHJWA_001632"
     CDS             51142..51726
                     /locus_tag="ACHJWA_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001056415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4308258.1"
                     /translation="MNMKNSSFLDEGKEPVIERIFRLAERYKSRSEAARAWGINVQTL
                     QNYYKRRNNQPPPQPRKHHLQNIAEREHVSMEWLVHGYGDEPSMKKEPIKQTKRTKKQ
                     RIDDSDSIIMRAWDSLTSSEQEILSNLLIRKGAELLTVLLDTDIQKLHSLSGVKRALA
                     LSLQELPDDTVREIYEECEAKVNHLNVTQKKASA"
     gene            51996..52307
                     /locus_tag="ACHJWA_001633"
                     /pseudo
     CDS             51996..52307
                     /locus_tag="ACHJWA_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310661.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            52312..52626
                     /locus_tag="ACHJWA_001634"
     CDS             52312..52626
                     /locus_tag="ACHJWA_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFO4308259.1"
                     /translation="MTTPTRRISFYLKPAAVKSEQEACNYLDSLPASERSRAQRAAFL
                     AGLALIKRNPAFAYWMAEWPEDNLPFNTVSIKETYQANQPSGVECNFYKIKKNIQTLF
                     PE"
BASE COUNT        12505 a        14739 c        12788 g        12625 t
ORIGIN      
        1 ataaaataac aaattatagt gacgccacag caacaaatat aacctttgtg gagcactatc
       61 atgctgaaac cagaaatgat tgaaaaactt aatgagcaga tgaacctgga actgtactct
      121 tcactgcttt atcagcaaat gagcgcctgg tgcagctatc ataccttcga aggtgctgcc
      181 gcgttcctgc gccgtcacgc ccaggaagag atgacgcata tgcagcgtct gtttgattac
      241 ctgactgata ccggcaattt accgcgtatt aataccgttg aatctccgtt tgctgaatat
      301 tcctcacttg atgaattatt ccaggaaacc tataaacacg aacaattaat cacccagaaa
      361 attaacgaac tggctcatgc tgcaatgacc aatcaggact acccaacatt taatttcctg
      421 cagtggtatg tttctgagca gcatgaagaa gagaaactgt tcaaatcgat tattgataaa
      481 ttaagcctgg caggcaaaag cggcgaaggt ctgtatttta tcgacaaaga actctctacc
      541 ctcgacacac aaaactaatg ctctccggcg gcaggtttct gccgccattt caaatcagtg
      601 acggcaaatc ttactctgat cggtcgaaaa accttcttct gaaggtataa atttgccttt
      661 tgctgccaga aacgctacca gctctcccgc tgtcatccct tctgccgagc aagtgtgaaa
      721 acgtgcctgt tcaccaaaac gcgctttaat cgcagcttcc agactggcat gcgtatattg
      781 ctcgcctgat tcaatcatca tatttaacac ttcatgaccg tgaatagagt ccatcgttcc
      841 tcctcaaaaa aaagcctagc gtagcgattg ccgcttatga agactttgcg ccagcgcagg
      901 actgaatgct ttttattgta catttatgtt tacaccatat gtaacgtcgg tttgacgaag
      961 cagccgttat gccttaacct gcgccgcaga tatcactcat aaagatcgtc aggacagaag
     1021 aaagcgtgaa aaacagaacc ctgggaagtg tttttatcgt ggcgggaacc acaattggcg
     1081 caggcatgct ggcaatgccg ctggctgcgg ccggtgttgg ttttagcgtt acgttaatct
     1141 tgttgattgg gctttgggcg ttgatgtgct acacggcgct attactgctg gaggtgtacc
     1201 agcatgttcc ggcagatacc ggtctgggca cgctggcaaa acgctatctg ggacgctacg
     1261 gtcaatggct gacgggcttc agtatgatgt tcttaatgta tgctctgact gcggcataca
     1321 tcagcggtgc cggtgaattg ttggcctcca gcatcagcga ctggacaggt atttcaatgt
     1381 cggcaaccgc tggcgtgttg ttgttcactt ttgttgccgg tggcgtggtt tgtgtcggga
     1441 catcgctggt cgatttattt aaccgttttc tgttcagcgc caaaattatt tttctggtgg
     1501 tgatgctggt attgctgcta ccgcatattc acaaagtgaa tcttttaacc ctgccgttgc
     1561 aacaggggct ggcactgtct gcaatcccgg tgatttttac gtcgtttggt tttcacggta
     1621 gcgtgccgag tattgtcagc tatatggatg gcaacattcg taagctacgc tgggtgttta
     1681 taaccggtag tgcgatcccc ctggtggcat atattttctg gcaggtggcg acgcttggca
     1741 gcattgattc aacaaccttt atgggattgc tggctaatca tgccggatta aacgggctgt
     1801 tacaggcgtt acgcgaaatg gtggcctctc cgcatgttga gctggcagtg catttatttg
     1861 ctgatttagc ccttgccacg tcatttctcg gcgttgcgtt aggcttattt gattatctgg
     1921 cagatttgtt tcagcgttca aataccgttg gtggacgatt gcaaactgga gcaattacct
     1981 ttctgccacc gttggcgttt gcactgtttt atccacgagg atttgtgatg gcgctgggtt
     2041 acgccggtgt ggcgctggcg gtactggcat tgattatccc ttcgctgttg acctggcaaa
     2101 gcagaaagca caatcctcaa gcgggttacc gggtcaaagg tggtcgtccg gcgctggtgg
     2161 tggtgtttct ctgtggtatt gctgtgattg gcgtgcaatt tttgattgcg gcagggttgt
     2221 taccagaagt ggggtgatta gatagcctca aattccttat tgggtgccag aattaacgct
     2281 gacacccaat ttgacttctt aatgcaggca gcactgctta aattttttac cactaccgca
     2341 cgggcaagga tcgttacgcc ccggtttctc ttctgctttg atcggttgct gaacagcttt
     2401 ttcctgcgga tgcgccatcc agtacgcatg tagatcaagc gccgccagtc gaatggcatc
     2461 tacgctctct tcaaacgctt ctggcgacat cttctctacc cgctcgaagt tttcctcagt
     2521 accgtgcagc gctatcgcct ccagcgctgg ttttaacgaa tcgggcaatg ttgaccagtc
     2581 agaaagtgcc acgccccgca tatagccaaa gcaccactcc tcaacaatcg tcagctcgct
     2641 gccatcaact tctcgcaagc cgaataacgg ctcaaactgc tccgggaatt cgttcagacg
     2701 ctctgcggta tcggccatat gttgaaaagc caggttcata aagcgcgtca tctctttctc
     2761 tgacgcccag cgcggcacat agtcagcccc accccacacg gcaaccagcc actgtgccgg
     2821 ttcaatctct tgcggagaac tcaacaccgc cgtcaataaa ccgtccagct ccgccacatc
     2881 aaggatggcg tggtcagtgt tgtatttggt cagaatatcg tccagccatt ccaactcact
     2941 ttcgtttaac ggtcccgttt tcatacgctt ttccttgtgg atctcaactc gccagcacct
     3001 atcttacatg ccggtccgta tcagagatac tttttgagtg gctttgccgg tgattaaaaa
     3061 tcaaggaggg tgtaacgaca agttgcaggc acaaaaaaac cacccgaagg tggtttcacg
     3121 acactgctta ttgctttgat tttattctta tctttcccat ggtacccgga gcgggacttg
     3181 aacccgcaca gcgcgaacgc cgagggattt taaaaactat caaccaccat ttataaatca
     3241 taaccttatg attttattaa ctttgaaaat ggctctatac aacgccattt gaatctattg
     3301 tcacttttta tcgccacttt ttaccctcta ttgtcaaaag ggttcaattc aacagcagcc
     3361 tctaaatgac ctggagcaaa atgcgcataa cgcatagtca tttttatatc gctatgcccc
     3421 agtatttttt gcaacacaag aatatttcca ccccgcatca taaaatgact ggcaaacgtg
     3481 tgacgtagca catgagttaa ttgcccatca ggaagctcga tcttcgctct cttaattgca
     3541 gcgtcaaaag cctcataaca tggtgaaaat agcgctcctc gttttttagg aagcatagct
     3601 tgcaattgag gtgaaatcgg tacagtgcgg ttcttctttc ctttagtttt aacaaatgtg
     3661 attcgaccgg gcagtacttg agattgcttt aatccttctg cttcactcca ccgagcacca
     3721 gtcgcaagcc caatacggac aacaaccccc aaatctttat tccgtgactc atcacacgca
     3781 atcagaaggc gttcaatctc atctacatac agaaacgcca gttccttttc ttcctcacga
     3841 aacttgcgaa taccagtcag ggggttttca ccagaccact ccccaaggcg cttcagttcg
     3901 gcaaaaacag catgtagata tgactgctcg cgattaacgg ttgcttcact aagttttttc
     3961 ttccccttgg gattccattc tcctgatagc cttctttccc gataagtagc aaacatattt
     4021 ttgtcaaact gagaagcaaa tggatctccc agcctggaac aaatcgcctc aagtttgact
     4081 ttgcgctctg caccagagga caaggtttta ccgtacatct caaaccaacg agcaatcaac
     4141 tcagaaagac gaggaccaga accatcttga aactcgtctc caactctact attcattaaa
     4201 cggcgctcat aagagagcgc ctcacttttt gtcgcaaact gtttacgaat gcgttttccc
     4261 gatgccccgt agggataaca ttcacaaagc catttacctt atggaatctt acgaaccgac
     4321 attttagtta cttatcacat aaatcaaatg cagccttagt gacatccccc agactctttt
     4381 ttaaccctgg ggcggcatca ttatctagcc aaaatggatt attgttatct aacggtaacg
     4441 caccaaatgt tttacctttt attcgagcca aacctgtaag tgcatataac ttattatcgt
     4501 caaaattcat cacataagga ttaccatcaa gacactgtaa ttgaacctca tcagtattaa
     4561 atggccatac cccattgaaa ctctcacgtt caatagtttt aaaaggcatt gcgacggcgg
     4621 aaaaagaaaa catagataaa aaagtaacta atagttgaac cttttttact ttcatatcat
     4681 tacctcaatt tagctcaagt aagattacaa attaaaaaac gccctagaaa tgacaccgcc
     4741 taccaaaacc cctacgcaga taaagaatat tatttctttt ggataaagtc ggattaattc
     4801 tgaagcacga agtcggactt ctggtaaggt cgaactctct atgtggcttg atgccgattg
     4861 ttgctctaac cacgacaatg cagactgtaa ctgagaacga gtaagatcgt ttaaacgtcc
     4921 tgtaccgaaa ttgatatggc aataccgcag aagtttttgt cgaagtccac agtcttcact
     4981 gttacgtagt aataaactta caagagcctt acaggcatca tgatctttac atcgctcaag
     5041 cattgcatgc agaaaactct ccgctgtttt atattgattt actgtcatat catcaatacc
     5101 agctacacca atctccgcat gtactttttg ccaaataata aacgcttcag tattgctagc
     5161 ttctgcaata gcagcaacca agctatttag ctccttacgc tgagccttaa gcaaagggcg
     5221 atcgtcatca tcattattcg aagggattgc gatattgacg gtatgagaac catcatatcg
     5281 ctctatctga atattctttt cgtgaaaatc acgcccagca acgcgattgt ttgaaccgtt
     5341 tgagttgacg gccatgtcac ctccctacta tcacctaccc ttagtttcgt tatagtcacg
     5401 accagcgata cggttattac caccagaaat atttaactca cgtcctgatg gctgagtttc
     5461 cttttcactg atcgcacctt ttaaagcccc aatcaccgcg tttttcacat ctaacgaagc
     5521 tgctcgaaag cgagtaatca attcctgctc atcatcgtta taagtttcag gtgagtgaat
     5581 tcccaacaca acatactgaa catcaaggcc aaaacgagac agcgctgcca aatacgcagc
     5641 atcaggaaag ctatctcctt tctcatatct aagctgagtt agctttttga ctccaccaat
     5701 gtcgctcatg gcaacttgac taagtcccaa tctttccctt tcctcacgca accgccgacc
     5761 aatatcattt ttcatacaaa aaccttgaca ggtatctttt ttgataccaa aatgatttca
     5821 cgagctatta gatgatcaca atataccact atgaaacaag ttcttcacga taccagatca
     5881 cgcattccgc gtaacaccgc cacaggtcca agactggcac ttcggctgtc cctcgaggag
     5941 cgagccgtca ttgatgaaat ggcagctaaa gaacaacgct catcctctaa catggcgcgc
     6001 atgatctccc ttcgcggcct agagctaacc cagaaagaac aaaacaaatc ttcctgatca
     6061 ggaggctagt gggatgtcag gtataaccat caatatcaat gtgaatgccc cctatgtatc
     6121 cctgcagaaa tatgcagaga taacaggtat ccctcttaat acatgcaaaa agatgttggc
     6181 tgacggtcga attattatcc gccccaaacg cgccaaaatg gaaaagcctg aagtaaacct
     6241 tgtggcgatg ttaaaagacg ctttggctaa cagctaaaac aatgaacaga gcaccatcat
     6301 gaaaaaaaac gctaataatc catactccaa atttcgtaat ggcgtagaac gccatgtaca
     6361 ccacgtcgct accagtgcat cacgtagtaa cagtcgctat aacctgaacg agacgcatgc
     6421 aacaccggat ggccacgctg taaaacaaat cggcgaacat gcctggctga ttgagaaagc
     6481 tggaatcgtg gtccacaaat gcccacgcaa tccgtttacc gggaaccgca tttttgcatt
     6541 gaactgcggc gacaatcact tcgggcagga tttcacatta tacgaagcac ttcgcacggt
     6601 tgatcgtctg cttcgcgggc aaagttttat taaacaggct gatttataac aggtgcttta
     6661 tgaccaaaga gcatgcacaa ggtgtattta tccgttttat tgattttcgc ggtgaactgt
     6721 tattacgcgc atcagccatt gacggaattg ttccatccga aaaaaacgca gctacttacg
     6781 tttatctgaa cggtacgcgc ctgactgtgg agcttccgta ccagaccgta cgagaaatca
     6841 ttagcgaagc tgaaaaggct cgtcaggtta atggcgatga accctatatc gaaattattt
     6901 gtatggattc agaagctgaa attcagaaag cagattaaag ggcgttgcga tgggcaaaga
     6961 atataaaact ctcattaaca aagcacctga acgattttat tttcgcttaa gtgcatcagg
     7021 cgctcatgct gaacgtgcag cccgtgactc attgaccagg gcaatccgga gtttgtatga
     7081 cgttgctttt tacgctgatg atctggatgc acttaacgaa ctttccgagc tgatctgtgc
     7141 cgcagaatgc ggggaacaca ttgaaccata taagctgggg aatatcgcat gagtatattt
     7201 atctcatggc ttgttctgat tatttcggtg gcctgcgcca ttgggattat gcgaattatt
     7261 cattcagtaa aaaagattga acgctttttc actgacaaat aacagcttaa acagaacacc
     7321 agattaaacc cgaaaacctg aaaactatcc gcattcgcgg aggtattcgc acacgtaaat
     7381 aacggagata taaaatgaac gcaaaagaaa aaaatattat caatacatta aaaatagtgt
     7441 cagcagagca agacaagctg tccagggcag cccagaaaga taatcaacat atggcagcgc
     7501 tttacgcact gaccatcgca atagccaccc ctgaagcagc caaagttatt gaggaacaga
     7561 gcaaagaaat cgacactctt aaaacacagt caacagttgc agccatgaat ccgtccagca
     7621 ttggacgctg catttatatt cttggttcag caatgatgct gcaatacacc attattgccg
     7681 aactgcacgg caaatacctg ataacgcctt accacacaaa agagtcagag cttctgacaa
     7741 atctccgcct gatagaacgc tctcaagctg tattcattga tgacgcgcaa cgtgccgtat
     7801 ttaacgcata gggttactgg acaaaggggg cgcaatggca attaagcatt ttcccgtcgt
     7861 tcgctttacc tccagagggc gcgaatacga ggtcgacgaa cgcctgatta ccactatcga
     7921 caaacatcgt tcggaaaagg atgcacacca catctacctc actgacggca cttacttctg
     7981 cgccaccaac gtggcgcgag taaatcttat ccgacaggta caggagccac gcagatgacc
     8041 attctggact acatcgctac tcatccgggg tgtagcggcg gagagatcgc cgcagcactg
     8101 aataccccaa ccacagccat taatgctgag ttacgccgac tttggcgcgg cggcttagtc
     8161 atcagaacaa accgcagcac aggtggtcgc gctcgcaaaa caggaggcca ggcttcttac
     8221 cacgtaaacc cgatgccgtt cgggtgtagc aatccactta ctcacatgtt taaccagcta
     8281 ctgaaggaag ccagagcatg agcaccatca accaccagaa gctacgcgaa ctggcatttg
     8341 ccctgcaacg aatggcaacg cctcaaaaat tactggcatt tcgcgcaatg ctctcgccgt
     8401 ctgctgtgct ggcactgctg gatcagctgg agcacgccag aaccacggct cctgccattc
     8461 gcctcacgct ccatcatgaa atcgctgatt tctgcgcaac gctgggggca cctggcgaac
     8521 cggaaacgcc ggaagcaatg cagcaagagc tgctgcaacg catcgataac gtttttgatt
     8581 tttttctgaa ccagtaagaa accagaacat gcacacacaa aaaaaccgct tgccatgccg
     8641 caatcggtca ggttacattt ccgctgcacc tcataaaacg ggtgccggga ttctcaaccc
     8701 gatacagagc aaagcgcata accgcgctag cggttttttt gtgcgtactg tattgccacg
     8761 tctttttcgc gtcagaatta tggcggggcg tacggggccg acttcggtcg ggccggattc
     8821 tttgctctcc ggtgttgaga accctgtacg tctcgccacc ccgagattct caactctgga
     8881 tggtgagtta tttctatcac cgagcaaaga ggccacacca tggcagaccg caaacagcac
     8941 cgcgctatcg cggagcgtcg tcacatccag actgaaatca accgcagact ttcccgcgca
     9001 tcacgcgtcg cgcaaatcat gcacatcaat atgctgcatg agcgcagcca cgcactatca
     9061 aacatttatt ccgcctctgt tttcagctat ctggcggatg atctgcacga gtttcaacag
     9121 ctcatccagc agcaaaacaa actccattaa ttcctgttcc gggcctttcc tgcaccttgc
     9181 ggcgggaggc cttcgcacat ctgtaacaag aggattgccg caatgattct cgccaacgac
     9241 tttcttgaat acctgctcaa cacagaacgt gatcttgccg ctcgcgtgcg tgatcgttat
     9301 gacatgtacc tgaaatccct gcctgtaccg cagctcgctg acggaaagat tgttattgat
     9361 ggtcgctaca tgattgacag ccacgaggga aattacaggc tttaccgcat tgaaggtggc
     9421 accccgtccg ttattggcat ttaccagcgc ccatcctctg caatcgtcga tgtgattgcc
     9481 gacagcatcc gcatcacaca tcgccatgcc gacacagaag acaccgtgct ggaaattcag
     9541 cggctggcta cagcctgccg cgacaccctg aatggcatga cgaagtaaat cactatgacg
     9601 gcagagtaca tcagggactg gcaacaaccg cgccacgcag tggggcgtga aggaacgggg
     9661 atccccgctc ctgaatccgc gctttcctcc tggctggatg cctaccgggt agagaacgag
     9721 cgccgccagg aaatggctga tgcggcgttc tccgcaacgc cgctgggcaa cctgattaat
     9781 aaaagcctgg acgcacagga aaaacaggac aaaaccatca cactggcagg agacgccaga
     9841 aaacaggcac gcggtgcggt ggatgaagcc atggcctcgc tgcgcctgct gccgtcctat
     9901 ctgcgcgatc cgcttattcg ccacctctcc ttcctgcgca aaaaacagga agccgatcgt
     9961 cagaaaggca aaaagagctg gcaggctgaa cgctacgcgc gcggaaacct gcgcaaaata
    10021 ttcgaacgtc tggagcgcac cgatcaccgc tggctgacac agggttatcg ctcccttgcc
    10081 ggacgcgaac gcctggacga tttgctttac ctgccgcagc tcaacaaaca ccagatacag
    10141 acgctggcca ccatgacggc ggcgatgttc agcagcacct tcgaaaaact ctgcgatggc
    10201 tttggcgcga ccgatggcga actgaccatg gatgtaacgc tgaaggcgta tcagatgctg
    10261 gcccgcatgg cgttacacct gcacgccatg cctccacatt atgacgcact gacaacagac
    10321 aaagaccgga ggaacgaacc ggacacggag ctgctgccgg gcgcaatcct tcgcctgacc
    10381 tgtgcggaat ggtggaaacg caaactgtgg ctgttacgtt gcgagtggag agaagaacaa
    10441 ctccgcgccg cctgtctggt ttccagaaaa acatcgccct atctgagcca ggacgcgtta
    10501 agcgagtttc gcgcacagcg cgagaaaaca cgcgatttcc tgaaaagttt catgctggaa
    10561 aacgaagacg ggttcacgat tgatctcgag acagtgtatt acgcgggagt aagtaacccg
    10621 gttcaccgta aggcagaaat gatggccacc atgaaggggc tggaacttct ggccgaagcc
    10681 cgtggcgaca aagcggtgtt tctgactgtc acctgcccgt caaaatacca cgctacaaca
    10741 gagaacggtc atccgaatcc caaatggaac ggggccacca tgcgcgactc cagcgattac
    10801 ctggttaaca cgttttttgc ggcggtccgc aagaaactga accgcgacgg cctgcgctgg
    10861 tatggcatcc gcacggtgga gcctcaccat gacggcaccg tgcactggca tatgatggtc
    10921 tttgctcatc cggaagaaat cgacaccatt gtgtcccaca cccgcgatat tgccattcag
    10981 gaagatcgtc acgagctggg cgatgatatt actccgcgct ttaaggcgga gtatgtcgac
    11041 ggctcaaaag gcacgccaac cagctacatc gccacctaca tcggaaaaaa cctggacagc
    11101 cgcgccgtgg atggcatcga cccgaaaaca ggcaaaccac gcgttgacca cgaaaccgga
    11161 aaatcaatga ccgagagcgt ggaacgcgcc attggctggg cgcgccttca ccgggtccgc
    11221 cagttccagt tctttggcat cccctcccgt caggtgtggc gtgaactgcg tcgccttgcc
    11281 agccagatgg cacgcaaccc ggaaggcccg caacggctga aggatgacgc aatggatgcg
    11341 gttcttgctg ccgctgatgc cggatgtttt accacctaca ttgagaaaca gggaggcgta
    11401 cttgttccac gcaaggacta cctgattcgc accgcctacg acctcgcaga tgagctgaac
    11461 gattacggcg aacagagcgt acagatttac gggatctggt caccactcat cggggaatcc
    11521 tcccgtgtgt gcacgcaccc ggataactgg aagctggtaa gacgtaaacc gggagtagaa
    11581 gacagcgccc gcgaaaatgg ttttgacctt cagggcggcc ctgccgcccc ttggactcgt
    11641 ggcaataact gtccccgtgt acaggaaacg gacaacaacg ggacagaaca gccggaagaa
    11701 cggccagcac cgtggccgca gcttcctgac ggcgttgacg tgaacgaatg gatgcgctca
    11761 ctgaaacggc acgaacgccg ggcgctgatg cgttcgcttc gtgacaaaca ggcaaaaaac
    11821 agcagtgatg aaatgcagag ctggacacag agccgcaaac agcagcggcc tttgcctgat
    11881 aaccacgaat tactcgctaa agaatggcgg gagtctgctg aatctctcgg cctgcatatc
    11941 ggtgaacaac agatgcagca cctgttacgg ggcggcagtc tgtacgttga cggcagcatc
    12001 attgcaccgc agggatttga aattgtacgc aaaccggata cccgcccgga cagccgaatc
    12061 acgcagctct ggcagcgcct gagccgtaat catggcgtaa gcagcacgga gatccgccat
    12121 aacccggtcg ccagctatct ggcacagctg ggggcatcag accctgaagc cgccgcacgc
    12181 ctggcatcca cacttcagca ggaccagaac accatgaaaa cacccgttac cgtgctttct
    12241 gacatgctgc gcgccatccg cgacgcagag cacgcacaga gaatcagtga aaccactgaa
    12301 cgcgccagcc gcaaagcaga cctgctgcgg ggtggcctga ccagtggaaa caaaaaacag
    12361 acagaaacgg gactcacaaa tcccgtaaat gagcaaaaaa cgcgccgcga tatatgaagc
    12421 gcgcacaaaa caggcaaaag cgggatttaa aaatcctgta accgattaat taatcaacat
    12481 aaggaaaagc gacatgaaaa tttgtatcga cgacggctcc accaacatca agctggcatg
    12541 gactgagaac ggcgaacgcc gcaacgccat cagcccgaac agcttcaagt cggaatggtc
    12601 tgcgccattc ggtggcacgc agcccgcgaa ctacatgctt gatggcgtgc gctatggttt
    12661 tgatccggtc agcgatcgct ttgtccagac gaccgacacg caataccagt acagcgatgt
    12721 gaatgtaatt gccattcatc acgcgctggt caaatcaggc atcacaccac aggaagtgga
    12781 tgtggttgtc accctgccac tgagcgagta tttcgataca aacgcacagc cggacatggc
    12841 caacatcaac cgcaaaaaag cgaacgtcat gcgcccggtg gagtaccaga acggcgaagc
    12901 attcactatc cgtaacgtac gggttatgcc tgaatccatt cctgctggct ttaaggcact
    12961 ggctgacatg agtccgtttg aatccctgct gattgtggat ttgggcggaa ccacgctgga
    13021 tgtggcaaag gttcaggggc aactggcagg tatcagccag gtgttttgcg atccacacgt
    13081 aggcgtttct ctgatggccg atgccgtact gtcggtgatg gccactaacg gtatgcgcac
    13141 cagtcaccta tagcgcggcg atttttttga taccgatttg catttcattt gataccgctg
    13201 aaaaacgccg atacgcaact tatttgatac taaatcggcg cttttctttt gagttttaga
    13261 atgagttttt aagcgctcat tcactctctt tcaaaccagt tttaactgtt tgtggttttg
    13321 ggtaaggctg gactttgaat agctttgtgt tgaggcgttt tctggccctc ttgttcagaa
    13381 accggatgta acgaaactgt cggaacttat gaacactggc tctgtcaata ttagccctca
    13441 gatgttcacc tcgttgtccg cctcgtttaa tggcattcct gcaaatctcg tgataccact
    13501 cgccatcaag ttcatagaat attgattcat ggctgcctac ataatcaaaa ttgcttgcct
    13561 gatacacgac accaagacag ccacaacgct catctgcaaa cgactgaacc cactgtacct
    13621 gcggataaag ctgcctgatt agcttgagcg cgtaactgat tgcccttgat tcggagtttc
    13681 tcggcataca gtcatgcagc catagccggt taagctccat atattctcgg ttctgcgtac
    13741 ccgttacgac gcgtgcacca ctgtttgggt taagggcgta accccattgc attacgccaa
    13801 caagttcccg ttctgaaaat atgcccagat gaaggtagga gttatttacg aaacggtggc
    13861 tgtaatgttt attgacgatg accagtctgg ccagccagca acttattgtc tcaacccgca
    13921 actcacggga gccataacca acgatattgt cgttatagcg aataagttca ggcgtgctga
    13981 tgattcggga tgtgacttgc tttctgttcc ccacgacagg atttccttgt gtatgtgggg
    14041 tgctctgtgg cgctcggaga tgtgatttga ttgagggttt tacagcgcgg acattttact
    14101 tccagataac tgaaactggc ccgtgccagt agcttattgc agtgtcggca ccgtatgttt
    14161 cgatacatga ccgcataacc tcctgctttg ttgttgatgc ctttctcatg caatcgatcg
    14221 acaaaaacga tcgattcgat ttgtttaatt gatacaacaa atagattgaa aagtcatcaa
    14281 caatgattca gggaggtgat tatttactta aagaaaacga agagttaggt ggtgaaaacg
    14341 atcataaaat gatcatttta ttgacttttt atacagtgaa ggtaggatca cgcgtatatg
    14401 tagtatgttt caaatcaaca ggatactaca taaaagtgaa acccagcata gctggaacta
    14461 tgctgggtca atctgtaaaa acttgcatca atgctttact tcgagctcgt ggcgtatcct
    14521 atgatattcc ccggtctcct gtaaagcaga gatgcataaa aaggagatct ttatcatgga
    14581 tgaaagagcc tgtttgccct gcctgtaaga ctcccttaca gctcatctat cgccgttctc
    14641 gatctcaaaa tggcaagaca tacaaaccca aaaatgcttc ctgtttcagg atgctgatat
    14701 gtcctgcctg ttctcccagg agggaaaata cacaaaacgt gcattaacaa actcacccgg
    14761 cagggaaacc tgccggtcgg tattgttaca tcatagtacc gtcagttccg ggctgaaccg
    14821 gctctggttg ctctacgggt tgaggatcat tttccgtgct gttttcagcc ggagaacggc
    14881 caaccattcg ggccagcact tcatcaacat atgcgtcgat ttgtgcctca aaatccttgc
    14941 gaacctgcgc cttcagcacg cggttcactt cttcagaata aagtgcctgt ttgacaagct
    15001 cttcagtaac cgtgccttta atctctggca tgaatattct ccttttgcag gtatattgcc
    15061 ccgttatcag agcggggtcg ggtcgtttgg ggatgacggt gcctgtgctg ttttactgag
    15121 tttttcagct gccgcatcat caattttacg gcgaatataa tttctgatga ccttataacc
    15181 accgctcacc agatataacg cactgaccac agcgcagaaa taaagcagca ttgtatgaac
    15241 gaatgtcata taaccttacc tttgatattg actacataat tcgttatcgg gtaaaaacat
    15301 cgaagcgact gataaccact ccttcgggag tatttttatt ttcctttatg ggttaggtag
    15361 ctcccgccag ccatatcatg accggcggga tttttttatt ctgttaccgc ttttttcact
    15421 tccaccacaa tgctgtcaat cagcaccggg taagttgcat tttttgtgat atccgttacg
    15481 cggagtttgt ccaccgggaa agttcctact ggtgactgcg tcagggtaaa cgcggcacca
    15541 tcctgaccat caataaccgg aataacctga aggctgttat ttcctgcaaa acggaaggcc
    15601 agcgtatgcc attcattatc aaatgcacca aaggttccca gtttctggtt atttgttgcc
    15661 acttttgcat tgtggtacat cacattcagg tcttttgcgt ctgtctggat gtagaacgat
    15721 gccagcagat tattccctgc atcccctgtc agggtgacac cctgcggcag agaggaaacc
    15781 ggccagtaaa gccccatgac atactggttt gccaccaggt cgccaggtac tttaaaacga
    15841 caactgatta aaccaccatt tttcagcagt tcagcaccag taccggcatc atgatccaga
    15901 taccaagatg atttacctgt ttccttggtc agtttcattg ccttaccacc tgtgcctcct
    15961 gcatcactga taatttcagc tttaccgcca gccggctccc agccctgggc tttcagattt
    16021 ccttctgact cactgacacg ataagacagc agtgttgtgg ttactgcttc agtttctgaa
    16081 ggtgacggca ctggtgcttc agtttcagaa gatggaacat cggcttttac tgtggttcgc
    16141 cccgcatgag tcagaactgc cgtggccaga cggtcggaaa taatcccacg acgtgcccat
    16201 gaactgaaat ggctggcccg gtccgcagat gtccaggtgg cggcactgtt acgccacgtt
    16261 gagccgtaat aaccaatgga ttcaatatcc gggtcttctg ccggattgtt ggtgggtaca
    16321 ttcgcgtcat tttcatctgt cattaatgga acgaaatgaa tgtttttatc tgttttgttt
    16381 ttgtagccgc catagattgc ctggtatgcc ggttcatttt tctgtttcca gaaatacgtg
    16441 gtatcgccac aaatccacgg aacgccagca gcactgccac cggtacactg ttccgcctgt
    16501 tcagccaggt ctgcgcggaa tttttccacc atcgccagaa acagcgcgga atgctccgtc
    16561 ggcttcgcat caatatcaaa ctcgccctgc atccacacca cagcaagcag gcggtttttc
    16621 gggttcttcg ccagtgccgc ttttgtccgg ctgattaagt ccttatacag cggcttatcc
    16681 acaccccagc gggctgagtc ctctgaagca cccgtgctgt cactgtacgt gccgtcagca
    16741 cccgccgtga atgcagaacc accacggcag cagggaacca gcagaatccc cgcatctgcc
    16801 ggaataaacg gcagcagttt tttggcaatg tgaagcccct gacccacggt gccatattgc
    16861 cctttagcca catccgcttt cgggtgattt tttccactca tatcctgcac atcatgcaga
    16921 cagtggtcgg ccagaatgat gtcgttatac tgacaggctt tacctcccgg cgtgactgta
    16981 ctgcgacggg ctaactgcat aatacgcggt tcggggcggt catatgtctc cggcagtggc
    17041 agaccttcac cgtaagccat accgtttgac tgaccagcaa gaaccaccac gtaataccag
    17101 gaaggggagc tggaagcagt ttcatttcct gacggacggg tatgtgtttc agcattatcc
    17161 cagtctgacg gttcagcagt accggcaaca tcagcactta acggaatgac cgcaccaata
    17221 cccgctccgg taatttccac actcggatca ccggatactg gccttaccca caggcaatcc
    17281 cgtgtattaa aactgatgag acagtcagca aatgtcattc ctccttttgt ttcggccgga
    17341 ggaaagggag cctcaagaaa tgccgctgta ccaccggaaa ctttaacagt aaagtttccg
    17401 ggcattgatg atagttgaac ccatttggac gtcataataa tcagcctgtt cctgaataaa
    17461 ctgaattatc atatccacag accgataaac gccattttgc gttaattcag taaaggccca
    17521 acaaagggag cgtcttattt ttccgaaacg ctcgcaggat aataacgata aagggttgaa
    17581 aggccgatat caaaaataag tgccacctgt cttcgactga cacccccggc cagtaaacgc
    17641 ccgacctgct cgtattgttc ctgcgtgaga cgacggggtc tgccgccggt tcgtcccctt
    17701 tcacgggcag ctgcaagacc agcccgtgtc cgttcgacaa ttaattcacg ctccatttct
    17761 gccagcgcac ccattatgtg aaaaaagaag cgccccatgg gggttgacgt atcaatgttg
    17821 tcagtaagac tacgaaaatt tatcccctgt tgctgtaatt catctgtcag cgcaacaagg
    17881 tggcgcatac tgcgccccag cctgtccagt ttccagacca tcagtgtgtc accgggtttt
    17941 agtttcttca gtgcccgctt cagtcccggc cgctgtgtcg ttttaccact cattttatct
    18001 tcaaaaatct gctcacatcc tgcacattcc agcgcatttc tttgtaaacc tgtgttctgg
    18061 tcatttgttg atacgcgtat atagccaatc agcatgtatt taccttcctt aaaaagaggt
    18121 ggaaaggcct tttaaagcag atatacggca tccctgaaac ctcggtttac gggatactgt
    18181 aaacagggcc gcgaatgccc tgccatcgaa cggagcggct gttgcagcga ataaactcgc
    18241 cactccaaga aatattaatg gtgtcccatt tgacggaacg caggatataa atattacatc
    18301 ggggatgacg cagtctgtgg ctgactcacg ttatgttcaa agcattcgaa gaggttcaag
    18361 gtcaacgatt ggaatgcaat ataatatttt tgaggttcct gacggatgcg tacttacagg
    18421 gcttgatgtc gctggtgatg gtaatgcaac ggtaaccgca tattatcgac ctgttcagtt
    18481 tctgatagat ggttcgtgga aaacagcttc cagcgcataa aagtgcagta gttcaaaaaa
    18541 cagattatct gaataaatat atgatgattt tgtcttttca acaaaaacaa ggtgaaataa
    18601 tggagcttat taacgtaaaa cgatattacc cggaacacaa accctatggt gaggatgttc
    18661 agtatttcca gagtgaggac ggaagggatt tttatgaatc tatcccgctt ttcacaaaga
    18721 aatacaaact ctgtatttcg cctgtaacgg gtattatctg ctctgttgct gaagatgtct
    18781 ctgctttata tccggcaggt tttactgttg ttgaagtgga cgaactgccg gaaggtgtga
    18841 atattgacgg taactggcag ttttctgatg gcctgatatc aaaggtgccg gtaaactgga
    18901 agacggttgc tgaaaagcgc cgctcatcgc tgctccagga agccaacgaa actgtggatg
    18961 actggaaaac ggaattaaaa ctggacatga ttagcgatga aaataaatta cagctgacgc
    19021 gctggatggc gtatatccga cagcttaaag aaatgcactt taatgatatt gccagtgagg
    19081 ggcattacca ggcgattccc tggccggaaa aaccggaata attcagaggg tgcattcagc
    19141 acccttttct gtggtttaca ctgaacttac cgttctccag gcattattaa tataaatctg
    19201 aaccgggcga taatagaccc caccgatatt gtccgcagac cgggagccgg tttcctgcac
    19261 gttaattccg gaaagcatac aaccggaagg tgcgctgtaa ttccatgaca tttcattgcc
    19321 tccgggtgaa tgatagctct gtgctccaag ctgaacattc tgtacatacc ggccatctgc
    19381 ggttgactgc gtcattcctg acgtgatgtt gatatccagc gttccgtcaa aaggaacacc
    19441 attaatattt cttggggtgg cgagtctgtt tgctgccacg gcggttccac ccgatggcag
    19501 ggcatcagca gccttattta ccgtttcccg taaaccgacg ttatcgataa aagcgccttt
    19561 atccggaatg tctgcgccgt tctgctccct gcgcaggtaa cggttatcac cttcgctttt
    19621 gctgtaaaca tccagattat tacgggcggt tcccgcattc tccaggtcgg caaggttctg
    19681 atccttcgcc agagcattaa cgtcaccggg accaagactg ttttttgtgg caagcgcccc
    19741 aagaccaaga taaccacggg caagacgctg ggcttccgca ccggcatcag caatttcttt
    19801 caggttgttt ttccgggaaa aggcattcgc agcgataatg gctttaatgg acagcgtgag
    19861 ctggtttaac tgcgccttat ccggctgaat actggcttca gccagaatat tcagcagttc
    19921 ggcctggaga atattcagcc agtcctgacc aatccagctg ataccttttt gtccgtcacc
    19981 ttcggtaaac caggtggtgg tattactctg cgccggtgcc agcgcaggca tattcgccac
    20041 gccggaatta ttatcaacat gaaacattaa atagtctcct cttcatggct gtttgcatac
    20101 acgtaaataa acgtctgcca tgcaggctta taacggttca gaatacattc cagcgcaccg
    20161 ccttcataaa tacgcagcgg cgtgagaata tcatccagca cattcatatt ccggtagccg
    20221 gtttcactgt taatcgtgac aatactgacc cactgtgaat ccggtgatgg ctgaatatct
    20281 atttcataac caaactctgc cgcaaaccgg atataaaatt cacggttaag agagggtttc
    20341 atccggtatt tattcccggc ataacgctga cgctcctgaa tggttgcgcc ggtcatatca
    20401 cattccggta accccagata acgctcccag tcttccagca gcagacggga tgaatcagga
    20461 aaacgttctg ccagcatctg gttaccggtc caggaaacac gctccgtgga atgactgagg
    20521 cccaggcaca aagccgcaag aacggatgac ggccgcttat cccatgccag cccgtcaggc
    20581 agcaactgca acagggcacg ctgatgcggg gtcagggtta cagccatgtg atttccccca
    20641 cggtcagcaa ctctgtatct ccggccatca cggacttcag cgggctgcga acttcaaaat
    20701 cctccagtcc tttcacccct gcaacagccc gccagaatga tgaaggcaat acaagtccac
    20761 ccggccgcga ttcgttataa agcaggtccg ttaatgcctg ttttacggca gcctgatttt
    20821 ccggcgtttt gggtacaatc ctgatctcaa acgccaccgg cttattggtc agcttaaaca
    20881 cgctgattgt cggcccaagt ggctgaccaa cgggctggcc cgtcgccgga tcatcatgac
    20941 tgcggatata atccgccacc cgcttcacat caccttctcc ggggaaaatg tcagggttat
    21001 tatcctgaac aaaagtcaca ccaacactac ccgcctcagg ccattcaggc ttacaccatg
    21061 cccgcgtcac gcccggtact tcccgtgccc agcgttcaaa atcatactgt gttccccctg
    21121 atggcgggtt ctgtacccgg aataccagcc tggacagcaa ctccggcacc gtttccacat
    21181 ccgcaccacc ggtgagtccc gtgccggtaa ccgtggctgt ctggttgatg cccgcctgtg
    21241 gcgtgataaa ggacaggcgg gttccggtcg gggtatttcc ggcgcgcccc gcactttccg
    21301 cctccacatt aacattcagt gttccggctt ttccggtcgc agagccggtg atgcggtaca
    21361 caacaccatc gcttcgctga aggagtacgc cttccgtgat ccctgcatcc gtggtcagca
    21421 tcagttgtac cggtccgtca gcgcgggaag ccggtttacg gatgacaccc cagaatgcgc
    21481 agtgtttcag gagttcggct tcatcagctt ctgtcggaat tatctgccgg gagatccagg
    21541 ccagatgttc atgctcctgt gctgacagac ccgccagtgc atacgcaatg gcattcaggg
    21601 tggtttcatt cacgcccggc tgcgaaccgg gcaggcgctg gctgatatcc tgttgtgtct
    21661 gtgtgatcag ttgcgccagt ggtaagggct gataaggcat cattccccct tcatatcagc
    21721 ataaaaaatc atcggatgtc tgcttccgtc cggcagggtg attttcacgg aaagcgaaag
    21781 gcgatcatgc cccacacgca tggcatgaca taccacggat gtggcagcac cgcttttatg
    21841 cagccacgcc agcgcttcat cagcataggc ctgtgcacgg ctgaccacgg aggacagcgt
    21901 cttttcacgg cttaacagcc agagacggga gccaatgggg cgctcccgga aactgtctcc
    21961 ccaccatccc cgacggtcac gggtgccgtc agggatttca tcggaatcca gcgcccgcct
    22021 gtccgtaaac agtgaaataa tgaccgcagt cagcaggctg ttatccgtca gaagatctgc
    22081 gccgttcagt tgcagggaac cacaaccctg atcccagacg acagcaatat cagccattct
    22141 ctggtcctcc cgttttgctg ccttcaccgt tatcgtgatg cacatgacct gaataggtga
    22201 tcccggcaat aaccgcttcg gacatcgtga acgccccctg tgatcttccg gtgccgtcaa
    22261 tggacacatt gcctttgaca tgcaggttct tatccactgt gatattaccg gtaaagtgag
    22321 cctccggtgt atccacctga acaccttcat cggcgtaaat ctccagcgtc ttacaggtca
    22381 caatgatccg gccgttcttt gtcagacgta tacggtgccc ctcatggtga tacacgccgg
    22441 tatctccggc ggtaagtccg gtcgggcgac tgcggcggtc ttccaccaca agcaccacgg
    22501 cctgatcccg ttgtccgcca gcacaggcca gaaaaacttc cgcaccaggc agggggacgc
    22561 tgatttgccc gtactgctgg gggcgttcaa cgtcatcaaa ggtttcacca tccataccgg
    22621 tgatttgcac attctggatt ttcagcgtgt ccgttgtgcc ggtcagtaca ccacgcccga
    22681 acagcagacg aacaccccgc atcacggggg ccagaaggcg gctgatgact tcgtcattca
    22741 tcattgaatt taatcccctg ttttttcatc tgtgcccgca cgaaggcgtc cacgtcaccc
    22801 gcatttccgc ttccgctgtt tcccggctct gccggaacaa taaagccgtc acggggtgcc
    22861 agaatcagac gggtggtttc gccgtgattt gcatccagcg aaaactccac ctgacagaca
    22921 agaagatcac gctcttccac tccggtgcgg ggagccgtga cagccgtcag caggttgata
    22981 tcccacaact ggccgttctc ccgaaaccag tgccgcacgg tggctgtcag gcgcacagag
    23041 cgggcaaccg cccggcgcat ttcccggaca gccctctggc gtgcaccgtc tgcatcaaca
    23101 gcatgatccg ccagcacaat tttcggtctg taacggtgga tggcactgtc tgtcaccgta
    23161 ccaactggtg cggccagcgt tccggctgtc agtgcatccc cggcatgacc accaccacgc
    23221 ccgtgccccc gtacacggta ttcactgtgg cgcagacggt gatccacgtt gtgatccaaa
    23281 tccagcagat tttcgcccag cgtcagcgtg tcgccacgct gactgccggc ctgagtgaaa
    23341 accagttcac cggcggcatt actggttacc aggacgcctc tgtgtctcgc ggcccgtgtc
    23401 agcgcatctg ccacggtttc tgaattttcc agtgtgaagg tggaaaaggg ccgggctgcc
    23461 gttgcatcat ttacctgcca gcgcaccgtg accccgaacg gagcacacaa atctgaagca
    23521 atgtgctcca gcgtgcggtt cctccactgg ctgcccggat gaatggctga acagtccacc
    23581 aggtcaccgg ttttatcccg tccgctgagc gtgatctgaa aacgcgtggc gctgatgcgc
    23641 tgccggacct gatccagcca gccagtgatc acaggctgac cgtcaatgcg caattccaga
    23701 gactgaccgg cacggacgga tgacggaagg cgtacaccgg gcatcattac ccccagttca
    23761 aaggagcccg ccagatgttc aagagagcga cgaacactca ccgtcagcca gccggaaaaa
    23821 atctcaccgc ccagatacag ttcaaccctg ctgctcacta atcacctcca ctgaatggcc
    23881 tccgggaatg aacagcggat caacaatacc gttacggcgc acaaaacgtt gccagccggt
    23941 actgttcccg gtggcacggt acagggtcac cagtgcaggc tcggtggtac gtacagtcac
    24001 tactgttgca ccgggtagct gaactccccg tgtattcaga tcctctgtca gtgccagtct
    24061 ggcatcacga agcgtcagcg ccgtggcggt atacccctgc ccggacagtg tcatcacctg
    24121 ccgttcgagc gcttcgctta agtcacggtt aatgcgctga agatcaactg aactttccag
    24181 ccagacaggc caggtacggt gggctgcgtc actgtccggc agggtttcct gactgagaac
    24241 ctgatccagc agttttccca cggtctgtgc ctgggccacc acggcagcac tctgcattac
    24301 cgcccgtatc agacgaatat tcttttgtgc tgccggtgtc agaccgatga gagtctgcgg
    24361 attatcctgc tcatccagca cgtaacgcag ggaagataat gtcctgtaca gttgcggcat
    24421 tccctgagag acggcggatg tcgtccggcc gatactgcca ccgggccggg tattcaggcc
    24481 tcctgaagac agtgacggca gcgatggcag ggtgatcagg gcactgaaaa gccccgcaaa
    24541 agatgaggcc atgcgggaag gtgctgtcag taatgccgtg gcattccctt tcagcgccgt
    24601 gaacgtggca gtaaaggcac tgatatcctg tacaatcccc atgccggaaa cggcattttc
    24661 cagggcggac accttatcac tgatggtttc ggtcattgcc tggacatcgt gcattccgtc
    24721 agagatcacc gtccagcccg cttccagggt ttcaaaaacc tgcccgagcg cggtggtact
    24781 cttctgctcc agaatggcgg ccgtgtcctg tgtgacagca ggtgcggtat catcacttac
    24841 cggcgtgaca ttaatggtga actcaaccac gccctgttca gcggcgttat aacgactttc
    24901 aaagctgttt atcagcacat ttaacgtgcc gtaatccggg tgaagcagct ccccggcacc
    24961 gggagcacga agggcatcac gcagacgatt tcgctgtgtc tgaacatcat cccccatcac
    25021 aagtacactg aacgtaaatt cagccagttt aggtcccaga tcgtctgcac caccggtttc
    25081 ccgtaacgga tattcccgac ggacaatgtt tcgcccacca cgttcgcgct gctcacgcca
    25141 gacgaggaac ggcacaccac ggaacgcacc acgaccgtca ccagaggcca taattccctc
    25201 cgttccagac attcacatcc agtccgtgac cggacgtgtc atcaatatca atactgcgag
    25261 cctgccaccc ttccggggca accagctcca cacgggcaga ggctttttgt accgcctgtc
    25321 cactgctttc atcatcactg ccccgtaatt tctgccaggc ttcagtcagc catcccccca
    25381 gatagttacc gagatagctg cccaccgtag aaccaatggc aatccccaca ggtccggctg
    25441 ccgcccccag cgcaccaccg gcaatactgc ctgcaagaga gccgaccgct tctcccttgt
    25501 ctgtggcact ggcgctgcca tccagcagaa ccggggctgc catcgcgcca gcccacaggg
    25561 cacctccacc cagccggcca gccagtccac cggcactgcg gaaaaagccg gagaaacgcc
    25621 ccagccccat gcggctaccg acagacgaaa gtaacccacc ggcgcgcccc atcatgcgct
    25681 cccagaaccc tgccccacca gaagagggag acggtggcac tggggacggt ggcaggggag
    25741 gcgtgaccgg tcttccgcgc cccggtccgc gtttcctgcg gccccgcccc tgacgtccgc
    25801 ctgaggatgt atcggtcgcg ccggtgccgg acagtcctcc tgtgggccag tttgttacaa
    25861 gtactggctg aacggctgcc ggatttaccc cggtaagaaa gtttataaaa cgttgccctc
    25921 gtccctgaga tgtgcctggt ggtgttccgg ccggaggaga gcgccggaaa ggtgaggtca
    25981 ggaaggccgc cgctgataat ccgtatccca gcaatgcagc acccggacgt ataatccccg
    26041 ttccgacagc ccgcgccatt ttcagggcac gggtggcgag atacatgtac agcaggtatt
    26101 tcgcggcggt ctgtgccccc tgaccaatgc gatccagcgc atcaccatag cccgcatccc
    26161 gcagagcctg aagcgtgtcg cgtacctttc tgatcgcctg ataaaaccct gtcactgcat
    26221 cacgggcata ctcaaagccc tggttcatgg ctgatgccgt gctttccgcc agggaatggt
    26281 aactgccatc agatttggca ctgtcaaccc atgaaagaaa tcccctgaga ttacccttca
    26341 gtttttcaaa tggcccactg tccatcacat cccgggcaaa ctggtcccag acgtcaccca
    26401 tcatggccgt caggccactc caggaattca ttgcgtcctt ttgtgctccc ctggcctgtt
    26461 ccgccagcgt ctgaaataac aggcgaatac tgtccggccc aagcagccct ttttcaccaa
    26521 gttttctgac cactttctgg tccacaccca gacgatcagc cagcacgcgg taggcattaa
    26581 tgccgtaggt ggcaagaata tttgcatccg ccgcctgaat actgcctctt gcatacatct
    26641 gtttcagttg cagggatgcc ccctgtgcat cggacagtga ccagccgccc acggcaccct
    26701 gatcctgaag catggtgaca aaatttcggg cctccctgtc actcatacca aagcccagac
    26761 tggacgtata ttcctgcatg acgccagcca gcccccaggt ggtttccttc gcattctgta
    26821 ttgcccacct gcggacatca tcagttttcg cccgatcccc tttgtaaagg gagttcaggc
    26881 gaatcatata ggtttccatc tccgctgccg ggcgaataaa tccccggtta aagccatata
    26941 cagcagcacc accggccagc attccgtaaa gattgctgat ccgcccgatg gtgccggtaa
    27001 tactgccctg aagacggtca aaatcagaag tgacatggcg gatactgccc cgcaccccgg
    27061 ccagcgtgcg ctgcatcctg cttccgagta catctgtttc ctgcccggca cggcgggcag
    27121 catttcccag gccacccagc cccgcctggc cggagcgtga gaatgccccc agctcctgtg
    27181 accactggcg ggatttggct gagatattgc cgagcaaatc aactatcaga gatgctctga
    27241 gatttcgggc catacttact gcttcgttct gatgattttt tctgtctgtc ggcagtgccg
    27301 gtaaagttgc gacaggggga ggttaagcgc ccattccgga ccgctttttg tcaccatccc
    27361 caggataatt gccgcttctt caatctgatc ccgacactgc gcctgatcgc ccccggtcag
    27421 ctgccagctt tccggcaagc gcggtatcca gcaggctgac cgctgccatc aggcgggaga
    27481 gatcgcgctc gcttaactgt ccaatctgac ggggtgacaa cggaccttca atgtctccca
    27541 ctgcggcaat ctgacggcgc agcagtgcaa caccgcgtaa cgacggagac gctatcagca
    27601 tcgggccatt gtttgtctcc accacgcgct cggcttctgc ttctgcgtca atactgtctt
    27661 tcgctgtcag ttcccggaac gtgacacgat attcacgggt ctcaccaaac ggcagaccat
    27721 caagcagatc cacatatccc tttgccagtt gctctgtcag cccggcagtg cgtggatcag
    27781 ccccctcaag tgcttcccgg attgcctcca tcacggctgc ctcggaaact gccgtttttt
    27841 tcttacgtgt ggtcatttta tttttcctga tcactgaaca cgggtactgg aggcgctggc
    27901 gaacttcacg gagatctccc cgccaccgtc aagcgaggcc ggttcactgc tccaggcctt
    27961 cgtcatcatg tggacttcgc cggtatccgc cacaaactcg atagtgaccg cagtccagtt
    28021 gttgatttca tcggcggctg gtgaaccttc tccgcctgcc ggaaatttgc agtccagcgt
    28081 cgcttcacgt ggtttctggc ggtaaccata gactttcgcg cctttcacca cttcacgttc
    28141 aaaaccagac ggagaaaatg tagccccctc aagcgtctcg tattcctgac cgttcacacg
    28201 gatagtggcc gtaccctgat actgttttcc gctcatgcct tacctcacaa aatgaaacgg
    28261 atctgtgctg ccaggaagcg gaactggtta accagatccg gcgtgcataa cacgtcaaga
    28321 cggtttcgat ctgacgcatt acgctccaca agcagattct ttttaaaggt gtccagattt
    28381 tcgaccagcc cgagttccac ccactcttca cccagcgaaa ttaactgaag cttcatgatc
    28441 tccggcgtca cgatattctg accggcacga acgggcgtcc cgtcatcagc cagtttatgg
    28501 cgcggaaaac gttgtgtgat aaaagtacgc agggaataac gcagccagga gagggtgtag
    28561 atggtttcca catccagata gctgggatca ctttcgccgt acacgttctg gcgatacatg
    28621 gtcacctgac gttcgatctg aaccacatca ccggcagcca cactgaccgt ggcaatccct
    28681 ccccacagca gactgttacg ctcttcacgc gtcagacgat cggccggagc cggggccatg
    28741 cgtgacggaa gcgccagcgt ctgaagcgga cgcgcaggat ctgctcccag agagggtgca
    28801 caggtggcac agaccgccgc tgcccagagg taatccggtt caggggcttt gggaatggat
    28861 gtgcaggtcg ccagaaaatc attgcgtttt gccccgaatg cctgcacctc accaaacgtg
    28921 ccggtatgtg ccagccacag cacgccgtca gacattttgg ccggtcccca gcgtttcaga
    28981 agctcatcac tgatgatttt caggtttgca tcatccttat agggcatgac gacatagttg
    29041 tactggcgat cgcccatacc cgccacgctt cgggtgatat ccgggttagc cgctttttgt
    29101 ttcggatagg ctatagcgac ctgtattcct tccggggttg cttccccgtc gtgatagttc
    29161 aggcgcacat catgaacgga gcattcaccg gtaaagcggg cggtgatccc cagagaccct
    29221 ttcagacctg cgccattatc accacttcct gcaccagagg aggccgtgaa gggcgcgtca
    29281 ggatcggcat tgatcagcgc ggccagctcg tcggtcagtt ctttgccttt cttacctttt
    29341 ggtgccggga gtgtataacg ccgccccccc acgcaggcat aaacaatccc gtcacgggtg
    29401 accgttccgg tcagattcat ggccgcagcc gttgcctgtc cggtgccgtt cccctgagca
    29461 atggcataca gttcagcatc aggattgatg gcgataaatt ccgccaccat cagggcaatc
    29521 atggagccgc gcccccagag ttctgttgcc tgagacgcac gggtgatcct gaccggaaca
    29581 tccagaacac cggcaccgtc cacggcattc tctttcattc tcgcctgacc aaacagcagc
    29641 acacactgac gctgtgccgg agtgccggtg acagccatgg agttattaaa ctcaacctca
    29701 atcagcggta tacggcggtc attaccgatc ccgtcaaaat taatcattgc ggtttctcct
    29761 tatcggatgc cggttgggcg accggttttt ctgtcgcagt cgttgcatcc gcagatacag
    29821 ccggagcctc ttcagcggct ttttcagcct gaatgacatc gccgtcatca agacgccgac
    29881 accagaacgg cgtaaagggc ttttcctccc cctcctgtgc cagcgggcgc attgtgtcgg
    29941 gatcacgaat caggcgtccc ggcgcgggtt tgataaagat ggttttcatt tacagttcct
    30001 ccggcggttc gaccgccgtt gtcgtgttcc ccggcaaatc aatatgtgcc tcaaacggcg
    30061 gcgtaccggg cggctccaca aaggtttcgt aatgacgcag gaagtcgtcc agcgagctga
    30121 tatcggtcag cggtgcgatc atttcctcgc aggagaaata aagcgcatac atcaccgcac
    30181 cactgtccgc ctgggtttcg gtgtaaccgt tgaccgcctt ttcaaagtaa agcggtgagg
    30241 ttttttctgt tctgaagccg ttaagcacgg caatcagccg ggccacaatc tgatacaatc
    30301 cgggacggct ggcctcacgc ccgttgagca tgtcgccgat gacgtagaac acccagtgac
    30361 tgaccaggcg accacgggta cggccttcac cggcacccag ccaggcaacg tagatcgccg
    30421 gggcgttaat cagcatggtg cgcagtacgc tgtcgctcca gtcgccggga tgcgtgtcaa
    30481 cagacaccag ttcattcccg aaatactcac ggatacgggc gatgtatgcc tgttcggttt
    30541 ccgtaatcat atgaagccct tctggttgcg cccgaatacc gctgcatcag actgaacctg
    30601 tggtaaatcc ccggattcag gggccgcacc gttcgtatcc acaccgaccg gcacattgcc
    30661 gttcatgaca tctttcagcc aggccagcgc ttcacggtaa cggtcacgcg cctgatcgga
    30721 ggctcgctga tcgcacaggt aataaaaggc aatcgcgcaa cagtgctgaa caagaacggc
    30781 cggaataacc tccagcggca acgtgtaacg ggcggacaga tagctgtcga taagggctga
    30841 cgcatctgtc agcgcccggt tcagcttgcg cgtgtccggc tcatccgaac ggggcacagc
    30901 cagtaacggc ctgagcaaat cctcgcggta acgcgcccgc atatcggttt cagtggcgta
    30961 attcatgcac gggcctgttc cgctttgtta ccggtctttt tccggggagc atcctgtccg
    31021 gcttcggctt cggcttcggc ttcggcttcg gcttcggcta aatctgtacc ggccactgca
    31081 tgaaccacac ccgccacgct ccccgcatca tcagcagacg tctgtagtat tcgcacagtg
    31141 aggcatggct cctcctccag tcgggcaagc tgctccgggg tgacctcaac ctgctgacgt
    31201 ccacgcgtga acaggaaccc cgcacgacga aacgccgaac gactgcaacg cacttctgcc
    31261 agcaccgtga cagaattgtc accactgacc tgaagacctg aatcatctgt tgctaccaca
    31321 gtgtgatgtt cattcatatt tgccctccgc aggggccggg ccgtaagccc ggctcaccgt
    31381 ttacagataa tccgcgacaa ccagctccag cttgcccttc atctcgttag agacggttgc
    31441 gtttccgtct gcgaacagtt cacgctccag cagctgcacc gcctgttttt ccagcgaggt
    31501 ggggacaaca atatgggtgg gtttaatgcc gagtttgcgg ccaccgtcag cggtaaattc
    31561 gcgcatggct gaccagccgt gccacagtgc atccagcgtc agcggtgact gcatcatgta
    31621 ggccatctgc cagaagccgt agcccacatt gcgacgggcg gatgcaccaa acacaaattc
    31681 gttatcggtg aatgcgcgac cttcatcgac tttggtctgg gcaaccagtt cagccttacg
    31741 gcgatcctga taaatcagcg gttttaccgc gcgggagcag tcaagcagat accaggccgg
    31801 accgctgtaa tcagcctgtg cgccgacaga gccggttttt gccacaaaca tattgctgac
    31861 catctgcgca tccccggagc catccacttt ggggtaaacc ggatgttcgg tatcaaagaa
    31921 gttctgaccg tcatagcagg ccgcactgat cccgtcacgc agggcagcaa agaccagctc
    31981 atccggttgt accgccgcag cgcgccccat ctcctggaac aacggggaat aaataccgag
    32041 gttgtcgtct tcgaagtcat cgcggctaat ggcgacagtg ccttcaaaag ttttgttcac
    32101 gatggcatag ccataggctt tcatcttctc gatgacgcgg gagccgatcc actcacggaa
    32161 ctgcgggaac tgccccagcc agccgtaggt gttggatttc gatgtggagg gcacggtcat
    32221 cgcaattttt ttatactgcg acggggccat ggacatcccg gcctgaaaat cagaacggta
    32281 gcccgtcatc agggcggtga tcatcgccgg tgtaatcggg gtaggcatta ttgcatttcc
    32341 tctttcattt tcaggaactc ggcttcggtt ttacccagca gtttggcggc cgcgatatct
    32401 tcagccgaca gcgcagcggt ggcagtcttt ttatccggca cggtcacggt gtcggtctga
    32461 agagaggtca gggccgcaac cggctggcgg gcgtcaagct gtgcagaaag tgccgcaacg
    32521 ccaatctgcc cgcccagttg ttccagatag ccgcgttcgc ttttgaagat gcgcccttcg
    32581 gattcagcct tgtccagcac ctgctccagc gtggtgctgc cgtgctgcgc cgacagcgca
    32641 acgtattcgg tacgcagggc gttatacgtt tcaacgggca cgtatttcgt cagatcaata
    32701 atgccgccag ccggtgtgcc ttttgcggtt tccagctctg ccgacagact ggcgacctgt
    32761 gttttcaggt tgtcgtgtgc atccgcacgg gttttgatcc cggtgagggc agacagcgcc
    32821 gccgtgccca gctccggcgt aaattcgtca ccgtcagcca cggtcagacc gagcgccgtc
    32881 agcaactggc gtaattgctc attcatggag gtatccttta aaggagggtt taaggcgtta
    32941 taaaggtcat ccgccgacag cgcggcgaca ggattcatgc cggtcagacc ggggtcaccg
    33001 gtaatagcca gcatccgaag ttcgacaggt tcgccggtgt tctcgtcata gccaatcacc
    33061 ggtgaaaacc aggggaattc gttattacgc agatgttcaa gggccggagg gttccacttc
    33121 gggcgcacct cgaagccacg ctcttcactg aagcgaaaat tctccggtga ggccatcaca
    33181 aaaccggcag ccggtgcctg atgcccctta atcagggtct ggtggttgta gtcaattttc
    33241 accggctgat tgagcgccac aacccgggat accatgcgct caacggtcgc acggttaatc
    33301 agccagccct cggccggttt ttccgggcga ccgtcacggg cctttacccg accggctggc
    33361 atgatctgac accagtcacc gtccccgtct gcggacaggc tgatggcatt cagaatggca
    33421 taagcaagtt tgggcgtgtt tttcgtcttc attccggcag cataagccgg gaattttcag
    33481 ggcgggtttt gcgggatttc aggaagtgct tccaggggaa aaatacaaag gcgcttcacg
    33541 cagattttaa agcccgttaa atacaggtta gaaaatcacg acacgcgcct gaagggggta
    33601 aggtaatgca tttgcatacc ttaaacaatt aaggcgtttc tgtggccttt ttaaagcgtt
    33661 ttctgatttc gtccatgatc tcctgctctg ccacctgatc aaagcccata tacgggcgtg
    33721 cgccaatggc cgccggtccc ggtggcatac caggcaggcc accccactga tgaatggccg
    33781 cataaggctc atttgatcca atcagcgccc aggtatcgcc ataatccgtg gtcaggcgtc
    33841 gggccagatc gccgttcagc gtcaggattt tgccgggcat gtatccctta cgggtacgcc
    33901 acttgcggta aggatctgac cagtcatgcc agcgttcgcc gtccggctct ttttcctgtt
    33961 caaacgccat ttctgacgat gacagaagac tcgccgccac actgcgggcc aggtctttcc
    34021 ctccacccac aaactgaagc cgggcaaaga cccgctggag acgcgtaacg tcaacaacga
    34081 cagcggcatc aatggatgac atattgcctc cgcataaagg atgaatataa aataaacagg
    34141 cggtcagtgt acgcttaact ggtaaagtcg gtgcctgcct ccgggtggat catgtatgcg
    34201 ggttcaaccc ccgccactga ccgttaatca atatctcctt ccagtacctc aagcattcca
    34261 ccccggatat ccgatttcag cttatccata ttgataaccc ggtaagcatt cacaatcaca
    34321 tccagtttat ccggctgacg cttcaggctg tacggcgcat taacggcaat ctgcacactg
    34381 ctatccttac tttccacgat atacatcaga ttgttgtggc gtttgtccca cagcaccgct
    34441 tttggctttg ccagcattgc cggtaatcga ccgaaatcct ccggcgtcag ggcgataccg
    34501 tcattctgat gtttcatgct gtccgcatgg agcacatttt taccgctcat cgccagcagg
    34561 cgggccggtg gcgttcccgt ccggctttcc accgcctgcg caatgctttc ggtcataaag
    34621 cccagcgtgc ggatatcgtt accgcctcgc cgggttttca tgatgttttt tgcccagatg
    34681 cggaacgcca gctgccgctc cgggctgttg ttcatctcct gcactaccat ttcccgcagc
    34741 gccgggcttt tcacctcgat cagtttacgg atcaacgcct ggtctgtgcc gaacgccgcc
    34801 gagccggggt tatatgacca gcccacatcc ggtgtcatgg ttctggtgcc atctgaatat
    34861 gtggtcaccg gcatttctct gacttctccg gtctgcttat ccacgccagc ctcaacattg
    34921 cgggtggaga gatgatcctg accggatgaa acagacagcc ccatcgcatc cagacgggcc
    34981 tgagacaatg gccggacacg gcagcgacag ttccagccat tgggcgggta gtgtgtattc
    35041 cagaacggat cgtcatagcg gaataccagc ccgttaaggg cggaatgtgc cgggcgggtg
    35101 cggctgtcca tcaccgccac atactgccag aacggatgcg tgtccgtgtt gttcatcatc
    35161 tgcgtgtaac gcccggcatt gtaagccaca cgggtgttca cgttgtaaat cagcgccaga
    35221 cggcggggac tgcccagctg cacttcttcc gcgttaccgg cactgtccac cacaatctgt
    35281 tttccccacc atcccagctt ttgcaggcgg ggtgtcagcg tgcgaataaa tgctttctgt
    35341 gaaatcccct catcaacagc acgctgcact tccgcctgta gtgtggtcag tacgtccagg
    35401 cgtgccactt ttgccgccgt gaatgaacgg gcatgaacat ccgcgtctgt ttcgaaccag
    35461 ttccagctga tatgcgcccc tttggcgcgg aagtatgcca ccgcctcttt tgaggcaagt
    35521 gtggcggcat aacctaaatc aatcccttgt gccatccagc atccccttca tttccgcagc
    35581 aaacatggca tcactgagca gcgtcatcag gcgggaatca tccatttcac ggtaaagggc
    35641 aggcaggtct gccagtgcct ccgccagccc ccgcgttctg atggcatcaa tgaccggctc
    35701 cagtaccggg tcgatggcct cctgtaaccg acgggcaggc accgcatccc ccatgtcgtc
    35761 cagttcatct cgggggctgc tcttcgcttc cggcagtctg gcagccagcg ctgtctgctc
    35821 tgtcttctcc tgtttttccg gtggcatctc tttgtctgtc tgagcaggtt cattgccact
    35881 ctgacggaca cggaagatgg cttcaccggg ggcaggctgt ggaataccgg tctgctcccg
    35941 tacccaggga tcaggaatat ccatccccgt actgagctgc atcaccgcac tggtgatttt
    36001 ggtgatatcc cccggctctt ttgtctggaa acagatacgc ggcaggcgac ggatatcaat
    36061 ggcgtgggcg gtgttcaggg catacagcgg atacaccaga tcgcggttca gcgtggcggc
    36121 cagctgacgt aaatcagaat ccctgatttc ccggcgcacc tcgttatgca cttcacccag
    36181 cgaacgtgcg cccttgtctc cggcttccgt ggtcagcgtg ccgccgagta ttgctttgga
    36241 gatggaacgc tccccccacg aaatcatggt ttcaaacgga tcggcctgac cgttcgcggc
    36301 tgcctgaaac tccagagaca tcccggccgg gatgatcccg cctgtacgtc gcccgatatc
    36361 catcaccgcc cgcatcaggg cgcttttctg ctccggcgtt gccccggacg ggtatttacc
    36421 gaccttcatc ggcaggccgt acacctccag aaattcagcc agatcgcgca cggaatagtt
    36481 tttgaaaatg aacggccaga taagcgttct gacaagccct gttgcgccgc cgtaaccggt
    36541 gcgtgaacgt gactgatgca ctatccagcc aaagggctga aacgccaccc cggcatggct
    36601 gccgtcacgc agccgcagtt cgctcaaatc atccgggtta aggcagaaat tcccgctgtc
    36661 acgccagcgg atggcgcgga tgatgtgcat tttaccgagc atcccgtgct caatctccat
    36721 gcaggaatag cctttcagga tggcatcggt ggcatcaaac agcatggcat caaaccagtc
    36781 ggcggaatgc aggtattcgt cgagcatttc cgcgtccttt ttctcattcg cgctggcgtt
    36841 cgggggcggc tcaatactcc agggcacccc ctgaatggca agacgtcgct tgcccagctc
    36901 tgcaaaaaga tgggtatcct tttcttcaat gtcagcggcc agatcggact gggcaatcag
    36961 atcgccacgt tcagccccgc gaaggcactg tgccgcccgg ttcggggtga tacccgaggc
    37021 cggatgttca atataacggc tggcaatctg cggaatatcc agcacggcac tctgcatctc
    37081 cgggtcaaag gagaaaggtt ttccgtcaag atcaattatg cgtcccacta ccagcacccc
    37141 cgatcaaatt catgatatgc ctcatcgtca tcacgataac cgccctccat ggcccgcgac
    37201 cgctccggca gcgcctggca ggctgattca tccaggataa agccctccat gtatgacgcc
    37261 cggtttgcca tacagagcgc cacggcaaaa tcaccatggc ggcgggcgtt cacagccgtt
    37321 gcgttctggt ccttcgtacg ccccttgtcg atttgcggga taccgttcac caccttcaca
    37381 tggcgcaaat catcaagcgt ggtctgatgg cgggcgacga ggatattctg atcctcaaac
    37441 tcggccttca gttttggcat ccactcgccg taccacttcg gcgataacat cacgcagtcg
    37501 atgatgtccg ggccaaaggc cagcagcgcg gcttcggcca gatagccgcc gttaccggtg
    37561 gcgtcgaacg ccgcaccgac cagcgccgga acgcgggtca ggatgtacat catcacctgc
    37621 tcctgctggg catacggcag gttgcgcagc tccacgcgaa acacctcgcg ttttgccagg
    37681 gattcagtga tttccagcag cacaaaacag gacaggtctc cggtgcgggc aaagtcttcc
    37741 ccgaagctga atcgggaacg gggatttaac gcctctaaaa gcggttttaa atgttcttca
    37801 caccaggtta aaacctccga ttcacgcagc catgcggcac ggctgatgaa gtcgtccggg
    37861 gcttcaaacg tcagaatcgg gatgtcgcgg atcatcgcca tttcaataag cgcgtgagga
    37921 atataggcac cgccggattt tttcgggata cagccgtatt cctcgtcggc atcctcacgg
    37981 gtcggggcgt ttttgtagag atcatcacgc cacttctgct cgctttccgg tgaccattcg
    38041 cggccggtga cataacagat acgacggtac agcccgtccg caatggcatc atccagggta
    38101 atgcggtgga cgctgtaatc cttgcgccct tcgcgggctt cctgaatgta ctgattaaac
    38161 agattatcga cgccgttatg cgtggagata atacgcacgc gcgcgcccca catggtaagc
    38221 gccattgccg ccttgagaag ctcatccagt gactcgtgga acgcggcttc atcaatcacc
    38281 acatccccct gaaggccgcg caggtttgac ggacgggaag acagtgcctg aattttgaat
    38341 ccactgttcg gaaagcggat catgtaggtc agaatttctt cttttttatc ccgatcccag
    38401 aaggtctgct catacacatc agtatctgcc agctggttaa aggcacggga gaacagtgca
    38461 caggcagaaa tatattccag cgccatctcc tgctttgacc ccacataaaa cacattgcgg
    38521 ccaccgcgcc gcttcggttt tgcagcagta atgacgttac gcccggcttc cgcccaggtc
    38581 agcccggtgc ggcgggattt ttccgcgatg cagacctcgc tttcatcctc aaaccagcgg
    38641 gcctgataat ccagaaatac cggcatatca ccgggcaaat caaggctgtc cggcacatcc
    38701 acgcccagca gggctttttc accggccaga tcaattttgc gggtggagac ggtattcgtc
    38761 agtaaggggg agagttcttc ggcgttattc atcagacttt acccaacaga atccccttaa
    38821 tacgggactc aagctgttca ctcatcccgt ccacgccgcg cagctcgtcg gtgacggcgt
    38881 ttgccatctc ttcagcaaat gcagccctga tttctttttc acgcttaatg ctgcgctcgg
    38941 cggcactttc cgcacgctgt gctgacagga ggatgtcctt aataagacgc atatccacgt
    39001 cgctgtcact gttcagggat tcggtcgcgg cacgcagacg gcggtacatc agggcgcggg
    39061 acatctccag gatcaatgca gtggtttcac cggttggctt gtcccccagt tcggccatca
    39121 tggcttttgt ctgctcgcgt aaatcacgca ggttacgggc aatcagctca ttacgggagg
    39181 cttcccggct gatggccgcc ggtgaaagct gctgctcttc cggcaggccc gcctcacgga
    39241 tcagacggtt gatttcctcg cgtatctgga cctgcgtcag acgtttttca cgcagcattt
    39301 ccagcagtgg cttacggatg ctgtcgggga gcaaatccac cttgcgtaca cggccacgcg
    39361 tcggcttgtc catcgttacc cccttgcgcg tggtttcttc acacccggaa cactggcgcg
    39421 gccttccgcc acatcctgcc cccggccggt cagttccgcg atgaaataac cgttaatcag
    39481 aacgcgtttg cgcaccagtc cctgctcggc aagccaggca atgtgggtgt gaacggtgtc
    39541 gcgggacacg cggtggccgt aatcatccag acagtcctgg agcacggatt cacccagttc
    39601 accgttgtaa tctgccagcg accgcagaat gaccagacgc tgatcttcag taatgaaatc
    39661 actcattcgt ttttttcctt accgcctgct ccagcagcag ttcgttctga taggagacag
    39721 agcggagcgt ggcgttcgtg gttttcagtt caccccgcag ggtcacgatt tccacattca
    39781 gacggttgac ctcagcttct gtcggcagtg acgacaggcg ggtttccacc ttctcaacgc
    39841 ggtcggagag tttttcaaag gcctcgcggg ggacaaacgt tttgcgcatc agcgccatga
    39901 atatcccgcc agcggttgcc gtcgccgaaa ggatcggcac aacataatct ttaacgatgc
    39961 tgacccacat gacgggcctc catgatgttc tggcagttca cacaacggat ggcatccggc
    40021 acagcaacaa gacgtgcggc ggggatctct ccgccacagt ccgcgcagac acgtctgccc
    40081 ggagcatcca ttgcccggcg cttacgtgtc agccggttac aaagggcgcg ttctgatccc
    40141 cgctccatca cagcctgagc acgatctgaa tcatccatac ctttttcctt acttcgtctt
    40201 tcctgccgga acagcaggcg ttccggccat atcagtaaat tcatgttgtc gccgcgcctc
    40261 cagacgacga atactggcct tgtccacgtt gcagttcttg atcaccgcca gcagttgcag
    40321 gttgtaagtg accgatgccc caaacgtgaa cggctccggc acaggaggga ccaggcagtc
    40381 agccagccat tcagcgggga tcggcaccgg cttcacaggc acgtatttca cagacggccc
    40441 ggcgcacccg atcagccaca tcatcaggaa caggggcaga ggccgcaggc actgccgcca
    40501 gcgaccggcg gacaacgctc tgctgcccct cgctgtgctg aatgttctgt tgtttttcat
    40561 tcgtggccgc ctcactgatt tgtgaaatga gttgcagggt gtgctgctga tttgccagca
    40621 cctgacgcgc ctcatcccgc tcacgcacaa gcacctgatt attgtcttca aggcgctgcg
    40681 tcttctggtg cattccccat atagcggcaa ccagtacagc gataatcccc agcgccgcca
    40741 tgcacgcccc ggcaatcagg gccggtaagg cattattcac cgcagccaca ggacaccccg
    40801 taacagcagt accgcacaca gcagcgctat catgagcggt cgccagaagc ggttcagact
    40861 ttcggcaagt cgtagcgaca taaccattca tccgccgaac ggcgggcctc aaggccgggg
    40921 agtttcaccc cctttgaata aatccagcgg atatactgct tacaggacgc gggcatctgc
    40981 ccggcattga tcaggcgcag aagcgtggag ttgcgaaagt tcgtttcacc ggcccagaag
    41041 atccacgaag ccagcgccac cgtctgacca cgggtaagcg gcacctgaac acgccgatca
    41101 atggccgcaa aggcccattt catgtccttt tccagtagtt ccaggcattc ttcatctgtc
    41161 agggtcatac ccggtttcac atccgggccg gtatggccgt aacagatggt gggtgtaccg
    41221 gtgggatcga tatacgtgga gttttctttc ccttcccagt aaccggtgta atgcgtggca
    41281 atcgtgaacg tcccggcacc ggccagaaca agggcaatca gctttttacg cagtggtgcg
    41341 gggagttttg gcattgttac gctgtctcat gatgttctct gagtcagcgt aacggtgaag
    41401 gagaggcggc aggctttgcg gaatatcaga aaagtggcgg ttgcgttctt gccagatgca
    41461 gacggcgctg ttcgcgtatg acggtataaa tctgggtttc tgacatccgg taatggcggc
    41521 gaagggattc aattttctcg ccccggtccc agcgcgaaaa tatctcattg tttcgcagct
    41581 cggcaaagag agattccccg accggcaggt aataaccccg tccgcccata taggctgcct
    41641 gagccgcagc caccttgcgt gccagtttac cggccagtac cggctcaatc ccctgacgct
    41701 gaagttctgc gccggtgact tcaaccagtt ctgacagggt ccgcggccag ttctttttga
    41761 gaacatcatc agggatgtca tcaaggcggt caataagggc gtgcagttgc tcactgtcac
    41821 caccaaacat gctcatctga gtttctgcca tatcagcctc cggttctcgc gttcccggta
    41881 agtttaaaat aaaaatcccg cgttgtggcg ggatttgggg ttaacgggga agtgattttt
    41941 catactgtct gcaaacggcg tcatacccca tcagcgtgcg ttcgggcatt cccatcgcgg
    42001 ccagcatttt gcggcggtgc catcgcttga gacgctccag cacatctgag gccagggcgg
    42061 ggtgttgctc cagccactgc cagtttgcca caccttcgcc gccgttctgc gcggccgtct
    42121 gcgatttcac ccagcggtta agagcggttt ccgcaccatc ggtgataaag ccctgccggt
    42181 gcatcacctt ccagatggca cgaattttgg cggtcaccgt acccggtttt aaggcccggt
    42241 taaccggctt ctggcgcact ttaaaaccgc gtttttttaa aacatccagc acgcgggata
    42301 actcatccgg tgacatatcc cggcagctgg ttttgccggt ggctgccagc agcgcggatg
    42361 tataggtctc ccggtcaagc tgtagatcgc gttttgcgac atgtattaag gtaatcaggg
    42421 aagcgcgatt cattatcgtg gctccataaa aaatgggcgg tgttaccgca ccgccatgtc
    42481 aattaacata aaaggaaaaa tctatgtcag atataaaaaa tgcccttgat gttgccgcat
    42541 tcacaatggc tggaaatctt atggtaaccc tgttcgacaa gggggttatt tcccgtgagg
    42601 aggcaacaca tattatcaat attgctcaaa aaagtgccgc accccaaagc gcagagggac
    42661 accaggatgg ctcatatatt aatacaaact ggcatttcga caatctccgg gcctgcatgg
    42721 gcattaccgc gaaaaaagat taaaaatttt tgcataatgt tcagatgtaa taattgctga
    42781 accatcaaca ccaagcctgc ggcgaacgta ttcctcgcgg gctattttgt tttccagaga
    42841 aaccgcttca ttaatcagat taatttcccg ctccattatc gacagtgcct ctctggctcc
    42901 aagattttct tttatcgcat attcgataat gtcacgcata cgttcaggca tgccgatttt
    42961 agccagttct tcgtcgatat ctataggcgc aaatttgatt tctttttcca tattattaat
    43021 cctcacaatt catacatgca gtgtcgtcgc ccatatcgca gtgattacat tttacctctc
    43081 cacaatgtgg gcactctttc agatactccg gggcttcatt tccacaggtg ggacattcaa
    43141 ctatttgatc atccattggg cgtctcctgt aattccggta aagatgaaat atggcaccag
    43201 tgcgtcacct gtccgtcttc atcttcaagg caggctccgg tttcttcatc aataaaaaag
    43261 ccttcatacc ccatatggcc gatgcgcagt tcctgttggt caccatactg aacaatcagc
    43321 acgacagcct ccatatcagg cggaaatgcg tcatccagtg aatgccaggg ataaccgcgc
    43381 aaatccccga aataaacacc agtcagcgtt ccgccacaga ccggcattcc ggcagcacac
    43441 acaagaggct cgtctgattc aacaaataat gtcgttatca tggcgttaac tcccgtaata
    43501 caacatcttc ggcagtccgg cgacggtgat aaataaaact caccgtgccc tcggataatt
    43561 caaatttctc gccgatttcc ctaaaggtaa gacgccgtgg tgattcagca tcccgtaatt
    43621 cacgaataag atgaacatca tcgtcaggta tacgggtaaa aggcagtaat tctccgtatt
    43681 ttttcatgct tacgccaata tatcgcgccc gattgtggac agaatcccgg tctcgcccca
    43741 gatattctcc tatttgctta ccggtcattg ttctggcatt ttttcggata aatttatctt
    43801 catcatgttt aaatctggga cgtttgcatt tgagcgattc cgggtaagta ccgcgcaata
    43861 aaacaatccg gttatataca ccccagtaac ttctttttac cctgacagca atatccttaa
    43921 ccggggtgga tggataaagc gcaatcagca gcgcatcttc ttcaggtgtc catgcacgaa
    43981 catgagcggg tgcacggcct tttcgcccca tgggttgtaa aatcatcata catcaccccc
    44041 tgactcgctt ttcggcagca taaaactcat caggcagaat ttcggtcatt ctctgttttg
    44101 cgggttttac gccgcactgg agataaatca catcgtcaac gcagaaccac ttcacgggac
    44161 catgcaatat tgctgagaag tcaatgccca gccagaacaa cagggcatcg gttctggcat
    44221 acgtgacggg cgaatattca cgccacagac tgttaagttc gtcagaggca acgcgcaggg
    44281 ctttgggaat acgtgatgtg cgcggtgtgc agctccagcc attcgacgca gtcggttttc
    44341 gccataagtc gcgatgaaaa ggatatttgt catccataaa gcgcagccct ttaaaacaaa
    44401 agccactgat accggataca aataccgacc ggcacgcaac attcagcacg gcctcaaggc
    44461 gttttgcctc atccttaact ttctggcagt cctgctgata tttttgccac gcggccagcg
    44521 cggaagggtt tgatgtttta aagaacatta cgccacctcc gcagatatcc cggcaggcgc
    44581 ggccgtccgg tcaacaatca ggtaacgcag aacgcgcgag gtgatattcc agccgcttaa
    44641 accgcagata atgacgccca ggcggatgtc gatatacgcc atcacaaact ggggcacgcc
    44701 ttctttcatg gcctgctcat caacttcagc caccacataa acggtttcac aggtcagtat
    44761 gccctgagca aattcccacg ccatcgacgg aatatcatgc ccttcaatcc acggcaggga
    44821 ttttctgaag ctgcaccact gaacatcatc agccgcagat tcctggctgc atttgcgacg
    44881 aaccggctgc accgggcggg gacgcggaat atcataaaaa ccgttacatt ccgtcagcac
    44941 gcctgcatca accgcatcac gcagaaaata aaccatggag gatggcggca tattcatttt
    45001 ttcagccaga acaccgcagg tcagacgtcc gtaaatacgc agccagtttt taaccccttc
    45061 aagcactttt gcatcaatca tgaattattc ctccgttatt gcgatgaatt cggaatgctt
    45121 gattgccatc aatgacggat gcagcttttc aggggaaaag gcattatctg attcaccgac
    45181 aggaatacga accacaacag cccctttgtc gccatagaac ccggcagaag agcacgccat
    45241 aactgtgcgg ccgttgctga taccaaaaac ctcaaaatag cagtccagtt tattaaccat
    45301 ccaggtactg aatgccggaa gctgttttaa cttctcgttt aagatattca gtgctttgtt
    45361 cagttttttt ccttctctga atctgcggtc gggcgtaact gaaaataaag tacggccatc
    45421 ttcatcaaat ttatcagaca acacccgttt atttcttccg acatcaacat cctcatgcac
    45481 cagtattgat ttgacaaaaa aatccccaca acaccaggag taactatatc ctctggcatt
    45541 ctgattactg agaaaatcat caatcaattt ctggcgctcc ctgccaacac tatcaagcca
    45601 ttcccggtga taatgacggg aatcgtgctc attcatttta aaataacgat atttaatact
    45661 catgatatgc ctcattcagg cgcgagcagt cccctgacgc aggcgccata attaaaatga
    45721 agtgatattt aaataattaa tgcggtgtta tttattcagt ccggcatcct gttcaaaagg
    45781 ctcgacataa aaactttcag cgcctttatt caccttaata ccggcaatcc ctttcaccgc
    45841 atccggctcc gccaggacgg cttccttgtt cacttcctct ttcgtgcgga tgaatcgctc
    45901 aagccccata cggcgcagca tttcaatcac gccttccaca tcacggctga cgctgcatga
    45961 tggcgttccc agccgccatg acaccgttcc ggtggtcaga ttggcagttt ttgttttgcc
    46021 gccgttcgtc agctcatcgc ggttggtttt acaccagtcc tgaatcccct taaaaagcac
    46081 tttgatttct tttttaaggt tttcaatctg cggcgtataa cgggcggtga tttctgccac
    46141 ttcatcattc atcgccgttt ccagccgcag cgcctctcgc tgaatatcgc ccagggtgcg
    46201 gatatcgcgg ctgacttctt cgcgggtctg cggtgccgcc tctgctgcgg cctttaattt
    46261 tgtaacgcgt tttgccattt tgtttttcct tattgcaatg tatctggtcc gttctgaaca
    46321 gaacggcgtt caacggagct gcttaccccc atggcctgca tggaaccctg tagttcatct
    46381 gtcagaaact gaataattgt ggggctgtga tatttaagaa tttgtgcata aacatcacag
    46441 ggtcctgccg gtctgttctc tgtatcacgt tcgattttta ccgtggcagt aacccctccg
    46501 tgatttcctt actatcatta ccggctggct ttacttcatg cattacgtgg tcaaattcaa
    46561 aaataacgcg tactttgctc atggaattac ccctgctgat taaattcatt acctttaata
    46621 accccggaac tcatgttcat atctttcatg agagctctgg ctttcattgc cacaagttca
    46681 gcaaagtctc tgctttccgc gagcataacc actccaaaaa tcatggcggg tccatcacct
    46741 ttctgcttta tcccggttga acgtatgtcc agacttaccc ccatgcgcac tgttccatca
    46801 gcgtttttat ggataacgtg ctccttgtat tcgaaaataa tgcgtacgac tttactcacc
    46861 ctctggtcct cgttctgttt ggataactga cccggcgtct gacctcacgg cagaaccgaa
    46921 tcatggcatt aaaggcttct tcctccgtca ccgcttccgg tatccccgga accagtaact
    46981 gctcctgttc atcacgtcca tgacgcgcca taacctcaat cacatgccgt accgtggcag
    47041 gctttccggc aactatcggc aatgcacctt ccggcaggac gtaaccaaac tcaataagac
    47101 cactcgacca cgcccatgca atgagatttt ttttcacctc acacctccca gtaaaccgtg
    47161 cagccgctga tgcgggtggc cttcacacgg cggcgcaggc cgttggtctg tatggtgatt
    47221 tcaatttcac cggttccctg cacgttgcct accggcggcg tggttttcag tgcaaaacgt
    47281 tgcggatagc gcttactgcg ttccagtacc gcaccggcaa gttcggtcag acggcgcgcg
    47341 ctgttcaggg agtcgaataa attcattttg ttaccgcagg attgcatatt catataacac
    47401 ctcatttagc tgacttgttt cgcaccaaca gaacggcata ttccgctgta aacttctgtc
    47461 gcaccttcgc gatcaagtcc ggcagatatc accattcgtg tgcggtcatt aaattcaaaa
    47521 agtaaatcgc cacattcttt attttcggcc agtgaaatca ccttcacgtt atcgaaatta
    47581 accaggtaaa agcgtccgta aatatcagga atattaaata ccgccataat cacacctgtg
    47641 agagtaattc agggttggta taaacctctt taaaggccgc attaatatgt ttttctgtca
    47701 gcgccgcgcc ttcaccgctg gcggtgagcc acgcctggtt aagcgtatgt gtcagaacgc
    47761 gtaacgctcc cggcttttca gcgatagcct gcatgacggc cagctcgacc tcaccactga
    47821 tcccccacgc cctggcaatg gccagcacat ccgccttttt ggccttgcga agttgttttg
    47881 tacgggcaag acggctgaac aggcgcgata aatcatcaaa ggcgcggcgt ccacctttaa
    47941 acaatccgcg cgggttacca ataagcacca tcccgatccc cgtggcgtcc tggattgcac
    48001 gcagttgttc cagaccgtca ataccaagat gatccgcctc atccacaatc accagtccac
    48061 gcgttcccat caggcgacgg cggatggcgc gggataatgc ccctttgttc gcgcgggtgt
    48121 aatcaatccc cagttcctct gccagctcca gcagacactc cgtgacgctg gaatgcgcgg
    48181 gcgacagggt gatcatccag gtgttcggtt gctcctggca gtaattacgg gcggtagccg
    48241 ttttacccac acccggtacg cccacaatga cgttaataca gcccatcagg cgaaccgcct
    48301 gaaacagtgc gcgcagctcc tggactgtct gcgtttccac aaactgcggc ggttccggca
    48361 gtgcgctttg tttattccag ttctcatacc aggaacgcag ggaagcagcc acagcagcgt
    48421 tatcgccttt atattttccc ttacggaaag ccgataatgt gccgtcggaa attcccgcct
    48481 ctctggcgat ggcatactgc gtcagtacgc cgccatcaat aagttcatca atggtcttga
    48541 ttacatcgtt aatatcagtc atattattaa cctctcattt gataccttgt ttaatcaaat
    48601 aacctgagtc gccgctcggg ttattttttt atttcaggcc agcgggtcat tttcttttaa
    48661 tttcgcttcc agcaactgca atccccgctg gaaattacgc tcgtattctt catcaggttc
    48721 atcgtcagcg accggttgct gaacggtcac cgtattaccc acagggcggt atatgttttc
    48781 cagccagggc tcctgcggct tgtgctccag cacgttgaca acctcatcct cggcatcacg
    48841 gattttttcc tctgcgcgtt tacgcatgcc tttaaggcgt tgctgctgtt tgtagtattc
    48901 cgcgctgacc gggaaggctt cgcgtttatt gccgtcccat accgcctcgc aaatcacgct
    48961 gccatcaggg cggcgcacgg taattcgttc ggcatcatga atgtcatagc tgataagcac
    49021 cttgcggcca tgctcatcac gcagctcggg cgcgtagtaa atattattca gccagcgtat
    49081 ttcacagcgt cttacagggc gttccaccat cggccggaac atatcccgca gctcaacatc
    49141 ggacagccat tcaatttccg tgtcctcttc cgccaggcgt ttttttctga actccgccgg
    49201 gctgtaatgt ttaccgttcg gcttcatcgg taattcatcg tgcggtcggt tgttgtacca
    49261 ctcaacacca tcacgaatgg catcaatcag ttcagaccag gacggtaaat cacgcatcac
    49321 tgactgttgc cgggcgttca gccgtttgcc ctgttgcagg gcggtaaatg ccgagcgtaa
    49381 atcgcggttg gttttgcgta acgtctcgcg atctgcacct ttcccgaaat aggtgcggta
    49441 tttacgggct atacgcatcg gtaatgtgcg gttaagccgt tcgataatgc cccgtccctg
    49501 cgggttaccg gcaatcccgg tcgggtgatt aatccccagt cgcggcagta tccccacaac
    49561 ctccttatcc aggatgtcgg cggtttcccc ggagccgtta tccgagtaat acagaaacgg
    49621 tttgccgtga tggcgaatac cgtgctgtat ggcaccggct acggcgaaaa cattttcagc
    49681 caggtcaagg ctccagccca ccacaaagcg cgtgccaccg tcgataacaa aggtcacttc
    49741 cggtgcaaat ggccgcccgt gaaccgggtg cgcacatttc agcttcatgc cgtgaccgtc
    49801 accaatccag acataattca ccggcatttt tgaccagtcg cggcgcgtga atccctcaag
    49861 ctggcggtat tcactgccgg tcacccggcc tttttgtttc accacttccg gcagcttctt
    49921 cattgcgcgg cgaatggtgt cataagaggg catgatatcg agcatataag gctcatcagc
    49981 gtgccggtgc tgccattcag caacaaaatc ctcgtaagcc tcggtcattg gtcggccgtt
    50041 tgactggcga tactgcgcca gaaactcggg caaccagcta atatcttcgg cttttatttc
    50101 ctggcgttta cccggtgcca gtaaaagcag gcgttcagcg gcgttctgcg ccttgttaaa
    50161 ggccgcaatc cagcgtttca gcgtgatttc actcaacgca cggctgtttc cctttttggc
    50221 gttcgccgtc tcaaccattg ccacaatgcg ctcgtccagt tgcgaacgtg ccaggttgtc
    50281 aacgataaac cggatagctt tcgcacagct gaaaccgggt tgttgcgcga ctttcatcac
    50341 ttcgctgacg atcgcgattc gtgcatcagc cacctggcgc tggttttcag tcagggcatt
    50401 gaggcgttcg accatcagtt gtggtgatcc gcgatatgcc tccaccgcat caaccacggc
    50461 aggtgagcgt ctggcctttg tcaccaccgg agccggtgat tcatcggctt tttgcgtcat
    50521 cagttgcagg gcgtagcgtt cacgtaatgc ctgctgcgtc acttcaggta aacagtcgat
    50581 actgtattca gttgctttag agcctgagcg acgacgggaa caacagcttt tcccttgtac
    50641 gtaacgttgt aatgcctggc gaataccttt tgttgtagca ggcattccgg gcgcgccaat
    50701 cagttctttt gcaatgacat acatatcatg acgcctttct catatagctc ttaacctggt
    50761 atcgactggg ccagatttct tcaggcgaca ccccaagagc atcagcaatt atttgctgat
    50821 acggtcggca aggagtacgt aatacgcttt tcagcgaatc cctgctgtaa ccagccttta
    50881 aagataaaga gcgcagtgat aagccagcca tgtgtaagcg cgccttgatg atttcaggcg
    50941 gccaatcgtg atctgaaact tctcttctac tcatacttcc tctattctaa aaagttaccc
    51001 gcgcggatat tcgtgcggat atctgtacgg gtaaataata gaacaaacaa aagaaccaaa
    51061 tcaaggtatt ttgttctttt tattctttgt ggtgtttttg ttattaatat caataagata
    51121 aatacaaagg aacctgagag catgaacatg aaaaacagtt cttttctcga cgaaggaaaa
    51181 gaaccagtga tcgaacggat ttttcgtctt gcagagcgtt acaaatcgag aagtgaggcg
    51241 gctagagcat gggggataaa cgttcagaca ttgcagaact actacaaaag gagaaacaat
    51301 cagccgccac cacaaccaag aaagcaccat ctccagaaca ttgcagaaag agaacatgta
    51361 tctatggagt ggctagtcca tggttatggt gatgagccaa gcatgaaaaa agaaccaatt
    51421 aaacaaacaa aaagaacaaa aaaacaaaga attgatgata gtgattccat cattatgagg
    51481 gcgtgggact cgctaacttc atctgaacaa gagattttgt ccaacttatt gatcagaaaa
    51541 ggggccgaac tactaaccgt tttgcttgat acagatattc aaaaactgca ctcattgagc
    51601 ggagtcaaaa gagcactagc attgagtcta caagagttac ccgatgatac tgtaagagag
    51661 atttatgaag aatgtgaagc taaggttaat catttaaatg taacccagaa aaaggctagt
    51721 gcgtaaggga gtggtgcaat ttttgtgctg tagtacactc ctttaaaggc aatctggaga
    51781 aaaacgtttt aagccttttt aaagccattt taagcgtcaa tccgtagtat caaaagtttt
    51841 gccgatttta gaataaatga ttaattattg cacaatcggt atcaaataga ctttaagcgc
    51901 tcatccagac atcacctgtt ttatttcagt atcttacagg tgatttcacc tcctttcact
    51961 aaaaccctcg ttggtatcaa atgattcacc tggttacaca ccacatcgcc aataccatta
    52021 tcgaacatcg ccatgatgaa gcctggctgc gccagcacat ccacaatgac gcgcattacg
    52081 ccagcctgat ggcggttatt cgtgaaaagg aagaaacact gaaacaacgc gtgatccgcg
    52141 cgctggcggt tttttcgggt tacgggcggg tgatggttgt cggtggcggg gcggagattg
    52201 tggcaaccgc tatccgcgaa gcctgcggag ttaatgcgac tttcatcgcg gacggggtgc
    52261 cacagtttgc tctggttaat gggttgtacg caatgaacaa ggagtaaacc aatgacgaca
    52321 ccaaccagac ggataagttt ctatctgaag cccgccgccg tcaagagtga acaggaggcg
    52381 tgcaattacc tcgatagcct gccagcctcc gaacgcagcc gcgcgcaacg cgcggccttt
    52441 ctggctgggc tagcactcat aaagcgcaac ccagcatttg cctactggat ggccgaatgg
    52501 ccagaggaca acctcccctt caataccgtt agcatcaagg aaacatacca ggctaatcag
    52561 cctagcggag ttgaatgcaa tttttataaa ataaaaaaga atattcagac cttattccca
    52621 gaataatctg ataattgtat gtgaactaat aaaatac
//
LOCUS       DAZFAB010000024        47683 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZFAB010000024 DAZFAB010000000
VERSION     DAZFAB010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47683
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.024"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            54..686
                     /gene="comR"
                     /locus_tag="ACHJWA_001635"
     CDS             54..686
                     /gene="comR"
                     /locus_tag="ACHJWA_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFO4308260.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTGKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(748..1287)
                     /locus_tag="ACHJWA_001636"
     CDS             complement(748..1287)
                     /locus_tag="ACHJWA_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO4308261.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDEHRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1514..2818)
                     /gene="ndh"
                     /locus_tag="ACHJWA_001637"
     CDS             complement(1514..2818)
                     /gene="ndh"
                     /locus_tag="ACHJWA_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFO4308262.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDRDAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVELTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKLLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3218..3760)
                     /gene="ycfP"
                     /locus_tag="ACHJWA_001638"
     CDS             complement(3218..3760)
                     /gene="ycfP"
                     /locus_tag="ACHJWA_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFO4308263.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3783..4808)
                     /gene="nagZ"
                     /locus_tag="ACHJWA_001639"
     CDS             complement(3783..4808)
                     /gene="nagZ"
                     /locus_tag="ACHJWA_001639"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFO4308264.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPEKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSTLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQVSLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISARLNQLHERW
                     QEEKAGH"
     gene            complement(4819..5643)
                     /gene="thiK"
                     /locus_tag="ACHJWA_001640"
     CDS             complement(4819..5643)
                     /gene="thiK"
                     /locus_tag="ACHJWA_001640"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFO4308265.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPACIAPKPHLYLRDWMVVDYLPGEVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5624..6265)
                     /gene="lpoB"
                     /locus_tag="ACHJWA_001641"
     CDS             complement(5624..6265)
                     /gene="lpoB"
                     /locus_tag="ACHJWA_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFO4308266.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6279..6656)
                     /locus_tag="ACHJWA_001642"
     CDS             complement(6279..6656)
                     /locus_tag="ACHJWA_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFO4308267.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6659..7018)
                     /gene="hinT"
                     /locus_tag="ACHJWA_001643"
     CDS             complement(6659..7018)
                     /gene="hinT"
                     /locus_tag="ACHJWA_001643"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFO4308268.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7149..7331
                     /locus_tag="ACHJWA_001644"
     CDS             7149..7331
                     /locus_tag="ACHJWA_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012138106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4308269.1"
                     /translation="MLPYISPLFPFLPANALKRHSLFGLHDKYECVYFSFAFAFAFAS
                     KIIRTLNITAHHPPPA"
     gene            7364..9553
                     /gene="fhuE"
                     /locus_tag="ACHJWA_001645"
     CDS             7364..9553
                     /gene="fhuE"
                     /locus_tag="ACHJWA_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFO4308270.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVNQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(9613..11046)
                     /gene="ptsG"
                     /locus_tag="ACHJWA_001646"
     CDS             complement(9613..11046)
                     /gene="ptsG"
                     /locus_tag="ACHJWA_001646"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFO4308271.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11341..12138)
                     /locus_tag="ACHJWA_001647"
     CDS             complement(11341..12138)
                     /locus_tag="ACHJWA_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO4308272.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(12149..13153)
                     /gene="holB"
                     /locus_tag="ACHJWA_001648"
     CDS             complement(12149..13153)
                     /gene="holB"
                     /locus_tag="ACHJWA_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFO4308273.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13150..13791)
                     /gene="tmk"
                     /locus_tag="ACHJWA_001649"
     CDS             complement(13150..13791)
                     /gene="tmk"
                     /locus_tag="ACHJWA_001649"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO4308274.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEIITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTRWVKELDA"
     gene            complement(13781..14803)
                     /gene="yceG"
                     /locus_tag="ACHJWA_001650"
     CDS             complement(13781..14803)
                     /gene="yceG"
                     /locus_tag="ACHJWA_001650"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFO4308275.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRYLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYITVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAIASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14806..15615)
                     /gene="pabC"
                     /locus_tag="ACHJWA_001651"
     CDS             complement(14806..15615)
                     /gene="pabC"
                     /locus_tag="ACHJWA_001651"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFO4308276.1"
                     /translation="MFLINGYKQESLSVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGITLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGGVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(15735..16976)
                     /gene="fabF"
                     /locus_tag="ACHJWA_001652"
     CDS             complement(15735..16976)
                     /gene="fabF"
                     /locus_tag="ACHJWA_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFO4308277.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(17064..17300)
                     /gene="acpP"
                     /locus_tag="ACHJWA_001653"
     CDS             complement(17064..17300)
                     /gene="acpP"
                     /locus_tag="ACHJWA_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO4308278.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17511..18245)
                     /gene="fabG"
                     /locus_tag="ACHJWA_001654"
     CDS             complement(17511..18245)
                     /gene="fabG"
                     /locus_tag="ACHJWA_001654"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFO4308279.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILVQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18258..19187)
                     /gene="fabD"
                     /locus_tag="ACHJWA_001655"
     CDS             complement(18258..19187)
                     /gene="fabD"
                     /locus_tag="ACHJWA_001655"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFO4308280.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQHGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIGKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19203..20156)
                     /gene="fabH"
                     /locus_tag="ACHJWA_001656"
     CDS             complement(19203..20156)
                     /gene="fabH"
                     /locus_tag="ACHJWA_001656"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFO4308281.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20224..21294)
                     /gene="plsX"
                     /locus_tag="ACHJWA_001657"
     CDS             complement(20224..21294)
                     /gene="plsX"
                     /locus_tag="ACHJWA_001657"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFO4308282.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21375..21548)
                     /gene="rpmF"
                     /locus_tag="ACHJWA_001658"
     CDS             complement(21375..21548)
                     /gene="rpmF"
                     /locus_tag="ACHJWA_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFO4308283.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21600..22121)
                     /gene="yceD"
                     /locus_tag="ACHJWA_001659"
     CDS             complement(21600..22121)
                     /gene="yceD"
                     /locus_tag="ACHJWA_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFO4308284.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            22320..22904
                     /gene="yceF"
                     /locus_tag="ACHJWA_001660"
     CDS             22320..22904
                     /gene="yceF"
                     /locus_tag="ACHJWA_001660"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFO4308285.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPHSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(23005..23964)
                     /gene="rluC"
                     /locus_tag="ACHJWA_001661"
     CDS             complement(23005..23964)
                     /gene="rluC"
                     /locus_tag="ACHJWA_001661"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFO4308286.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDDGLKRCLQKLRNAR"
     gene            24537..27722
                     /gene="rne"
                     /locus_tag="ACHJWA_001662"
     CDS             24537..27722
                     /gene="rne"
                     /locus_tag="ACHJWA_001662"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFO4308287.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPATPA
                     QPGLLSRFFGALKALFSGGEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETREGRQQAEVTEKARTADEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEETV
                     VAPVAEETVAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVTAVKPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(27917..28870)
                     /gene="flgL"
                     /locus_tag="ACHJWA_001663"
     CDS             complement(27917..28870)
                     /gene="flgL"
                     /locus_tag="ACHJWA_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFO4308288.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDNDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEVNGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(28882..30525)
                     /gene="flgK"
                     /locus_tag="ACHJWA_001664"
     CDS             complement(28882..30525)
                     /gene="flgK"
                     /locus_tag="ACHJWA_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFO4308289.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNSQHKAGFDANGDEGEDFFAIGKPA
                     VLQNTKNNGNVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(30591..31532)
                     /gene="flgJ"
                     /locus_tag="ACHJWA_001665"
     CDS             complement(30591..31532)
                     /gene="flgJ"
                     /locus_tag="ACHJWA_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFO4308290.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTTGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(31532..32629)
                     /gene="flgI"
                     /locus_tag="ACHJWA_001666"
     CDS             complement(31532..32629)
                     /gene="flgI"
                     /locus_tag="ACHJWA_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309485.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFO4308291.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAIA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(32641..33339)
                     /gene="flgH"
                     /locus_tag="ACHJWA_001667"
     CDS             complement(32641..33339)
                     /gene="flgH"
                     /locus_tag="ACHJWA_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFO4308292.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(33392..34174)
                     /gene="flgG"
                     /locus_tag="ACHJWA_001668"
     CDS             complement(33392..34174)
                     /gene="flgG"
                     /locus_tag="ACHJWA_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFO4308293.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(34312..35067)
                     /gene="flgF"
                     /locus_tag="ACHJWA_001669"
     CDS             complement(34312..35067)
                     /gene="flgF"
                     /locus_tag="ACHJWA_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFO4308294.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADITPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(35087..36292)
                     /gene="flgE"
                     /locus_tag="ACHJWA_001670"
     CDS             complement(35087..36292)
                     /gene="flgE"
                     /locus_tag="ACHJWA_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFO4308295.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPSVNAFDASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGAEPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(36317..37012)
                     /gene="flgD"
                     /locus_tag="ACHJWA_001671"
     CDS             complement(36317..37012)
                     /gene="flgD"
                     /locus_tag="ACHJWA_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFO4308296.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNNGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(37024..37428)
                     /gene="flgC"
                     /locus_tag="ACHJWA_001672"
     CDS             complement(37024..37428)
                     /gene="flgC"
                     /locus_tag="ACHJWA_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFO4308297.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(37432..37848)
                     /gene="flgB"
                     /locus_tag="ACHJWA_001673"
     CDS             complement(37432..37848)
                     /gene="flgB"
                     /locus_tag="ACHJWA_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFO4308298.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRNATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            38003..38662
                     /gene="flgA"
                     /locus_tag="ACHJWA_001674"
     CDS             38003..38662
                     /gene="flgA"
                     /locus_tag="ACHJWA_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFO4308299.1"
                     /translation="MLAIKRSVAIIAILFSPLSAASNLTSQLQTFFSSQLAGISDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLGAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            38738..39031
                     /gene="flgM"
                     /locus_tag="ACHJWA_001675"
     CDS             38738..39031
                     /gene="flgM"
                     /locus_tag="ACHJWA_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFO4308300.1"
                     /translation="MSIDRTSPLKPVSTIQPRETTDAPVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            39036..39452
                     /gene="flgN"
                     /locus_tag="ACHJWA_001676"
     CDS             39036..39452
                     /gene="flgN"
                     /locus_tag="ACHJWA_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFO4308301.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEIAVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGSKKISI"
     gene            complement(39492..41027)
                     /gene="murJ"
                     /locus_tag="ACHJWA_001677"
     CDS             complement(39492..41027)
                     /gene="murJ"
                     /locus_tag="ACHJWA_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFO4308302.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGVLQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(41137..42060)
                     /locus_tag="ACHJWA_001678"
     CDS             complement(41137..42060)
                     /locus_tag="ACHJWA_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO4308303.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYCELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITEMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(42062..42709)
                     /locus_tag="ACHJWA_001679"
     CDS             complement(42062..42709)
                     /locus_tag="ACHJWA_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFO4308304.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVELTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(42720..43304)
                     /gene="rimJ"
                     /locus_tag="ACHJWA_001680"
     CDS             complement(42720..43304)
                     /gene="rimJ"
                     /locus_tag="ACHJWA_001680"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFO4308305.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            43540..44748
                     /gene="mdtH"
                     /locus_tag="ACHJWA_001681"
     CDS             43540..44748
                     /gene="mdtH"
                     /locus_tag="ACHJWA_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFO4308306.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGDAIGYIGGGWLFDLGKSVHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            44812..45459
                     /gene="grxB"
                     /locus_tag="ACHJWA_001682"
     CDS             44812..45459
                     /gene="grxB"
                     /locus_tag="ACHJWA_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFO4308307.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            45593..46153
                     /locus_tag="ACHJWA_001683"
     CDS             45593..46153
                     /locus_tag="ACHJWA_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO4308308.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVALTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            46259..47305
                     /gene="pyrC"
                     /locus_tag="ACHJWA_001684"
     CDS             46259..47305
                     /gene="pyrC"
                     /locus_tag="ACHJWA_001684"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFO4308309.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILHAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSVDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEHQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            47379..47624
                     /gene="dinI"
                     /locus_tag="ACHJWA_001685"
     CDS             47379..47624
                     /gene="dinI"
                     /locus_tag="ACHJWA_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFO4308310.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
BASE COUNT        11491 a        12893 c        11878 g        11421 t
ORIGIN      
        1 gtgattattt ttataataac ctttcattca cagtcaccag gtacaacggc atcatggcaa
       61 ctgactcaac acaatgtgta aaaaaaagcc gtggccgccc aaaagtgttc gacagggatg
      121 ccgcgcttga taaggccatg aaattgttct ggcaacacgg ttatgaagcg acttctcttg
      181 cggacctcgt cgaagcgacc ggagccaaag cgcccacgct atacgcggaa tttaccaaca
      241 aagaggggtt atttcgtgcc gttctcgacc gctatatcga tcgttttgcc gctaagcatg
      301 aagcacagct gttttgtgaa gagaaaagcg tggagtctgc gctggctgac tattttgctg
      361 ccatcgccaa ctgctttacc ggcaaagaca ccccggcagg ctgcttcatg atcaacaact
      421 gcaccaccct ctccccagat tcaggagata tcgccaatac gttgaaatca cgccatgcga
      481 tgcaagagcg cactttgcag cagtttttat gtcaacgaca agcgcgcggg gaaatcccga
      541 cccactgtga cgtgacacat ctggcagaat tccttaattg tattattcag gggatgtcga
      601 tcagcgcacg cgaaggtgca tcgttggaaa aactgatgca gattgccgga acgactttgc
      661 gtttatggcc cgaactggtg aaataagtcg caaaaaaagc ccctcagcct gagcgaatga
      721 ggggccaaat tgcagagtac agccttgtta tactttgtta ttcaaaatca gttgcccgtt
      781 actatctagc gggatctgcg tacccggatc gcggtccatg cggattttgc cctgctgatc
      841 gccaatctta taggtcacat cataaccgag cattttttct gacttgtcat acaccgtttt
      901 acaacgctgt tgcgtagtcg tgtaagtatc gctttcctgg agagagccct ggatctggtt
      961 accggcatat ccaccaccca gcgcccccac aacagtggcg acatctttac cacgaccacc
     1021 accaaactga tgccctatca cgccgccagc aacagcgccg agcaccgacc cggtaatgcg
     1081 atgttcatcc tgcaccggtc gacgatgggt cactgtgacg ttgcgacact cctgacgcgg
     1141 tgttttaacc gtttccttga ttggggttgc agaaacaacc tgagcgtatt gcgggccacg
     1201 ttcaaacacg ttcagactgg ccactgccgc tacgccaagc gcagctgcga caccaatccc
     1261 gatacccgcc aacattgatt tattcacggg aatacctcct tctttgcagt tatgccaatc
     1321 atgcaagcga aggagaaaag cagctatcag acagaagatg aaaagcgcgg gaatggctgg
     1381 aaatacttaa ggattcggag gagtctgaag gaatgcctga tgcgacgctt aacgcgtctt
     1441 atcaggccta ccgctcgtac catttgtcca gggcggatga ggcgtttatg ccacatccgc
     1501 cagtgtacgt cgattaatgc aacttcaaac gcggacggat aacgcggtta atactcccca
     1561 ccagcatcat taatccggtt ttaaagtaac catgcagcgc aatctgatgc attcggtata
     1621 gcgagatata tacaaagcgc gcaattcgtc cttcaatcat cattgagccg cgcgtcaggt
     1681 tacccatcag gctaccaacg gtggagaagt tcgacagcga taccagcgaa ccatgatctt
     1741 tatactgata atttttcagc agcttaccgt tcatctgcgc cagaatgttg ttcattgcgc
     1801 aagtcgccat ctggtgtgca gcctgagcac gcggcggaac aaagccccct tccggacgcg
     1861 ggcatgacgc gcagtcgcca atagcgtaaa tgtctggatc gcgggtggtt tgcagcgtca
     1921 gttccaccac cagctggttg atacggttag tttcaagacc accgatatct ttcaggaagt
     1981 ctggcgcttt gatcccggct gcccatacca tcagatcagc ctcaatatat tcgccatctt
     2041 tagtgtgcag gccgccttca tcagcactgg tgaccatggt ttgcgtcaga acgcgaacgc
     2101 caagtttcgt tagctcgttg tgggccgcag cagagatacg tggcggtaat gcaggcaaaa
     2161 tacgttctcc cgcttctacc agcgttacgt tcagggcttc gttggtcagg cctttgtaac
     2221 cgtagctgtg cagttgcttg accgcgttgt gcaattcagc ggagagttct acacccgtcg
     2281 cgccgccgcc gacaatcgca atgttcactt tgccatttgc gcccaggttg gcggagtatt
     2341 tcaggaagag attcagcatc tcctggtgaa agcgacgcgc ctggtgcggg ttatcgagga
     2401 aaatgcagtt ctctttgaca cctggtgtat tgaaatcgtt agaggtgcta cccagcgcca
     2461 ttaccagggt gtcataggcg attttacgtt ccggaaccag cagttcacct ttctcatcgc
     2521 gcagttctgc aatagtgatt gttttcgcgt cacgatcaat atcaatgacg gaacccagct
     2581 ggaactggaa accatgattg cgcgcatggg ccagatagct caacgcatcg acgccttcat
     2641 caagcgagcc agtcgctact tcgtgcagca gcggtttcca caggtggctg tggttacgat
     2701 cgaccagcgt aattttggct tttttcttgc gtcccagctt atgccccagc tgtgttgcca
     2761 tttccagccc accagcaccg ccgccgacaa tcacaatttt tttcaatggc gtagtcaacg
     2821 tgaccccctt aaatttatta accaattgtt aattaatggt tattaacata gctcttaatt
     2881 aacaacaggt tacagcaatg aaaatgttct tcgctattga gaataacatg aatggtgcat
     2941 tggtcatacc aaaattgatg ttaatcaagt tttgttgctg aaaagatagg cgactttacc
     3001 gggtgagatc aaaaaacaga gtttattgtt tattatttga gcgaatttgt gagagtgata
     3061 tagcgtgagg ggtgtatgta gggcgttcgg taggtctgat aagaggcgca tcaggcattg
     3121 atgctcgact gtcggatgcg gcgtgaacgc cttatccgac ttacagaatt cggagcgcat
     3181 caggtaccgg caaggttacc tgatgcgacg agggcattta cccgagggtt ttgaacgctt
     3241 taatgcgctg caaatgcggg gagatattct tgaatttgtg cgtctgctct tcgtcccaga
     3301 caatctcgta ataatgatgc aactcttcag atgtccgctg gctgttaagc gcttcatcat
     3361 tacgcgacaa aatcaccagg caacgatcgc gattcttctc gcggaagttg gtcacacact
     3421 tagtggcaat atcggcatac tcttccgggc gatcaatctt gccttccatg ttctcataag
     3481 ggaacaaatt agggttgaag atcacctggc ggatatcgca aagaaaacca atccgttccg
     3541 cccagtatcc gcccagacca acgccgcaaa tcaggggacg ctcgtcgacg ttcagttgca
     3601 acattttgtc cacttctttg agcagatgct gcatatcatg tttcggatgc cgcgtactgt
     3661 agcttatcaa gcgtacatcc gggtcaataa actgcaattg taagactttc tcgtggttac
     3721 ccggactgtt agagtcaaaa ccgtgtaaat agataatcat cgcttcctca cataagccag
     3781 ggttagtgac ctgctttctc ttcctgccag cgttcatgta actgattcag acgggcgctg
     3841 atcgctttcc agcgagccga gtccatcagt tcctgtcgcg aaaatgaacc tttatgatac
     3901 aaacgtgtaa cacgttctgc cttgatcggt gacagattat ctaacacgct gacggcccct
     3961 ttacgattat tgcagaccag gatcatatcg caacccgcat ccagtgatac ctgcccgcgt
     4021 tcggcataac tgcccataat cgcggcacct tccatcgata aatcgtcaga gaaaatcacg
     4081 ccgtcaaaac ccagttcctg acgcaaaacg gttttcagcc agtaggaaga accgctcgcc
     4141 ggacgcggat caacatcact gtagatcaca tgcgcaggca taatggcgtc gagtttattt
     4201 tcgcggatta acgtgctgaa aaccgacata tctttggcac gaatttccgc ttgcgggcgt
     4261 gggtcgcacg gcgtctcttt gtgtgaatct gcagttactg caccgtgtcc tgggaagtgt
     4321 ttcccggtcg ttttcattcc ggcttcatgc atcccatcaa taaaccgact ggcgattgcc
     4381 agggcttttt ctgggtcggc atgataagaa cgctcgccaa tcgccgcgct gatatgtcct
     4441 acatccagca ctggcgcaaa gctgatatca atatccatag cgatcatttc gctggccatc
     4501 agccaacccg cctcttgcgc cagtttgccg ccctcttcca ttcccaacag cgcagcaaag
     4561 gattgtgctg ccggtaagcg ggtaaaacct tcgcgaaaac gctggacgcg tccaccttcc
     4621 tgatctaccg ccaccaccag atgattgcgc gatgctgcgc ggatctggcg caccagttca
     4681 cgcaactggg caggatcatg atagtttcgc gtaaagagaa tcagccctcc caccagcgga
     4741 tgcgccagta tttcacgctc ttccgcgtcc agttcgtaac cttcgacatc caacattact
     4801 ggacccacac tgctctcctt attgttttat taatagctgc cgccaggtgt catcggccag
     4861 cctgataaat tgttgatcgc cggtttgtcg ccagcggtac tcaaaccacc ctgctttgag
     4921 catcagcagc cagggaaacc atcgcctgac ctgacgccat aattgcgccg gataaatctt
     4981 cgcgcgagtg gcatagtcat tgaccaattg ccggtgctgt tcagtatttt ccacccacac
     5041 cgccgccagt tccagcgcga tatcaccatc tcctgcatac tcccagtcga tgagttttaa
     5101 ccctgacgcg ctatgcacta aatttccggc gtggacatcc atatgcaatg gacttaagcg
     5161 taaaggccgt ggttcccgcg ctttgcgcag acgttttaac attcgcagcc aacccactgt
     5221 ccgccgcgcc ggatcgcttt gctgccagta cagttccagt aacggcaaca gcgttattcg
     5281 ccagccaaaa cgtggttgtt gatgtagata atacagcaag cctgccagtt cgttggtatc
     5341 cggcaaatac gtttttacct cgccgggcag atagtcgact accatccagt cacggagata
     5401 taaatgcggc ttcggtgcaa tgcatgcggg tagttgtgat aaagcccgat actggcgcaa
     5461 gaacgcggac tgcggcgcat caggatcgtg cggctgacgc acaacaaaac gctggccctg
     5521 atgttcaatg agaaaactac cgccactaag cccactatta aacgtcgtga cgggatgaaa
     5581 ctgcgggaaa aagcgcgaca gcaattcgtc gcgcgtgatg ggattattgc tgcgaaacgg
     5641 cacctttacc tgaccagata atttcgcccg tctgcaccag catcagctgc atttgtaggg
     5701 tcggagcgtt aacgttgcca gaggcgctgg agtacagcac gtaatgagcg ccgacattgc
     5761 gggcaatgcc tatggcttta ctacgggtgc ctaaactatc ctgcggcgac aaacctaact
     5821 gttgcttagc catcgacagc tgctgggcgg aaaccagggt aaatttcccg ttattagcca
     5881 gcgcatttcg cagcgtttcg gtcgcttctg cggcattcag cgaaccgtta gtacggttgt
     5941 taacgctatc aaccagcagg acgctacccg cagtcacccc gtcagcccca agcatcttac
     6001 tgaccatcgg ctgcattgcg ccattccagt cataatggcg aatatgcggc gcaggcggtg
     6061 cagtttgatc ttcgtgctca attgggcctg gctgctgcgg gatcgtcggc accgagggca
     6121 ctgtggggac aggctgttgt ggctcggctg gttgttccgg cgctggtttc acttcttcta
     6181 ccggtgcagg ttcacgttgc cccacacacc cggcgagaaa catcgccagc gcggtaatca
     6241 aggcgtagcg actcattttt gtcatcaaga ttcacccctt acaaatatag ataaagtctg
     6301 actttgtgcg cccccagaaa attggcgctg ccgtacagcg ttaccgccga atgtgcggga
     6361 atggtaacgc tgcgtggcct ttccagagga tgcatctcca gccctctggc gtcataccag
     6421 taaaaacgat aatgaacggt aacgggttct tgcctttcgt tataaagcgt tgaggatgct
     6481 gaaggttgaa tatcagacgt cgaaaggacg ggcttttctg cactgatgcc cgcagccaat
     6541 aaagatgact ccatcaccag cgattgctca tcattcaccg gaatttccgg atgtgaacga
     6601 cagcccacca gcagtaacaa cgccagagac accagcccaa agcatccttt tctcatcgtt
     6661 acagaccttt atgcgccagc attggtccca gcggacggcc acccaacaag tgcatatgga
     6721 tgtggtaaac ctcttgtccg ccatggcggt tggtgttcat gatcagacga tagccatctt
     6781 cggcaatacc ttcttgctca gcaatttttg ccgctacggt gatcatgcgt cccagcgcct
     6841 gctcatgctc agctgagacg tcgttcacag tcggaatgag gatattcgga atgatcagaa
     6901 tatgcgttgg cgcttgcggc gaaatatcgc gaaacgccgt taccagatca tcctggtaga
     6961 cgatatcgga ggggatctca cgacgaataa ttttgctgaa tatagtttct tctgccacga
     7021 cattttcctt tttcaaatag ccatgcgttg cgccatgcta cgccgcgcca cactgcgagt
     7081 atagagtatg gtcgagttaa acctgctctt tcaaccttaa ctcacgattt cttaagcaaa
     7141 aaatcgtttt gctgccgtat atatctccat tattcccatt tctgcctgcg aatgcgctga
     7201 aaagacattc tctgtttggt ttacacgaca aatatgaatg cgtatatttc tcatttgcat
     7261 ttgcatttgc atttgcaagc aaaattattc gcacactaaa tataaccgct catcaccctc
     7321 ctccggcatg agcctgacga caacataaac caagagattt caaatgcttt caacacaatt
     7381 taacagggat aatcaatatc aagccatcac caaaccgtca ctacttgccg gttgcatagc
     7441 actggcacta ttaccttctg ccgcttttgc tgcaccagcc actgaagaaa cggtgattgt
     7501 tgagggttca gccacagctc cagatgatgg cgaaaatgat tacagcgtaa cgtctacctc
     7561 tgcgggtacc aaaatgcaga tgactcaacg tgatattcct cagtcggtca ctattgttaa
     7621 ccagcagcgg atggaagatc agcagttaca aacgctgggc gaagtgatgg aaaacacgct
     7681 ggggatcagc aaaagtcagg cggattccga tcgtgctctt tattattccc gcggattcca
     7741 gatcgataac tatatggttg atggtatccc cacctatttt gaatcgcgct ggaatctggg
     7801 cgacgcactt tctgatatgg cactgtttga acgcgtagaa gtagtgcgtg gcgcgacagg
     7861 actcatgacc gggacgggta atccatctgc ggcaattaat atggttcgaa aacacgcgac
     7921 cagtcgtgaa tttaaaggcg atgtctcggc ggaatacggt agctggaaca aagaacggta
     7981 tgtggcggat ttacaaagcc cactcaccga agacggtaaa atccgcgcgc gaattgtcgg
     8041 cggctaccag aataacgact catggctgga ccgctacaat agtgaaaaga ccttcttctc
     8101 cggcattgtc gatgctgatt taggcgatct tacgacgctt tcagccggtt acgaatatca
     8161 gcgcattgat gttaatagcc caacctgggg cggtttaccg cgctggaata ctgatggcag
     8221 cagcaacagt tacgatcgcg cacgcagtac cgcacctgac tgggcgtaca acgataaaga
     8281 gatcaacaag gtctttatga ccctgaagca gcggtttgct gatacctggc aagcgacact
     8341 gaatgccacc cactctgaag tcgaatttga cagcaaaatg atgtatgtcg atgcctatgt
     8401 aaacaaagcg gatggtatgc tggttgggcc atacagtaat tatggacctg gctttgatta
     8461 tgtcggcggc accggttgga acagtggcaa acgtaaagtt gatgcgctgg atttgttcgc
     8521 tgacggtagt tatgaattgt ttggtcgtca gcacaatcta atgtttggtg gcagttacag
     8581 caaacaaaac aatcgttact tcagttcatg ggccaacatc ttcccggatg aaattggcag
     8641 tttctacaac tttaatggca atttcccaca aaccgactgg tcaccacaga gcctggcgca
     8701 ggacgatacc acacatatga aatcgttata tgctgccact cgtgtcaccc ttgccgatcc
     8761 gctgcatctg atcctcggcg cacgttatac caactggcgg gttgatacgc tgacttacag
     8821 catggagaaa aaccacacca cgccttacgc tggtctggtg tttgacatca atgacaactg
     8881 gtcgacctac gccagctata cctctatttt ccagccgcaa aatgatcgtg acagttcagg
     8941 caaatatctg gctccaatca ccggtaacaa ctacgagctg ggtctgaaat cggactggat
     9001 gaatagccgt ctgaccacca cgttagccat cttccgtatt gagcaggata atgtcgctca
     9061 gtccaccggt acacctatcc ccggcagcaa cggcgaaacc gcctataaag cggtggatgg
     9121 gacagtcagt aaaggggtgg aatttgaact caacggcgca attaccgaca actggcagct
     9181 gacatttggc gcaacgcgct atattgcaga ggataacgaa ggaaacgccg ttaatcctaa
     9241 tctgccacgc accacggtta aaatgttcac cagctatcgg ttgcctgtca tgccagagtt
     9301 gacagtcggc ggtggtgtta actggcaaaa tcgcgtgtat accgacaccg tgacaccgta
     9361 tggcaccttc cgcgccgagc aaggtagcta cgcgttggtg gatctcttca cccgctacca
     9421 ggtgacgaaa aacttctcgt tacaggggaa cgtcaataac ctgttcgaca aaacctacga
     9481 taccaacgtg gaaggttcta tcgtctacgg cgcaccgcgt aatttcagca ttaccggcac
     9541 gtatcaattc tgaataacac ctgtaaaaaa ggcagccatc tggctgcctt agtctcccca
     9601 acgtcttacg gattagtggt tacggatgta ctcatccatc tcggttttca ggttatcgga
     9661 tttagtaccg aaaatcgcct gaacaccaga accagcaacc actacgcccg ctgcgcccag
     9721 tttcttcagg ccagcctgat ccactttaga cacatcagca acgctgacgc gcagacgggt
     9781 aatacatgcg tcgaggttag taatgttttc tttaccacca aatgcagcaa ccagagccgg
     9841 tgccatttcg ctggtacctg tcgcttttgc atcttcagtc gcgtcttcac gacccggcgt
     9901 tttcagatcc agtgctttaa tcagcacgcg gaagatggtg tagtaaacaa tcgcataacc
     9961 gataccgacg atcgggaaca gccacagttt gctgctgtta ccagacagaa cgatgaagtc
    10021 gatcagaccg tgcgaaaacg acgtaccgtc acgcatcccc agaagaatac agattgggaa
    10081 tgccaggcct gccagaatcg cgtggatgat gtacaggatc ggcgcaacga acatgaagga
    10141 gaactcgatc ggctcggtga taccggtcag gaacgaggtc agcgccgcgg agatcataat
    10201 accgcccact ttcgcgcggt tttctggttt agcagagtgc cagatagcaa ttgcggcagc
    10261 tggcagaccg tacattttga acaggaagcc accagacagt ttacccgcag tcgggtcacc
    10321 cgccatataa cgcggaatgt cgccgtggaa aacctgacct gctgcgttgg tgtattcacc
    10381 aatctgcatc tggaaaggta cgttccagat gtggtgcaga ccaaacggta ccaggcaacg
    10441 ttcgatgaaa ccgtaaatgc caaacgcaac taccgggttc tggtaagcag cccactgaga
    10501 gaaggtctgg attgcagaac caatcggcgg ccaaatgaag gacagcacaa cgccagtaaa
    10561 gatggcagcc aggccagaaa tgatcggcac aaagcgttta ccggcaaaga agccaagata
    10621 ctcaggcagc ttaatacggt agaaacggtt aaacatgtac gctgcgatcg caccggagat
    10681 aatccctccg agtacgccag tatccgccag gtgtttagag gcgatttctt cagcaggtaa
    10741 atgcagtacc agtggcgcaa ccacggccat ggttttaacc atgatgccat aggcaacaac
    10801 tgcagccagc gcggatacgc catcgttatt ggtaaagccg agggcgacac cgatcgcaaa
    10861 aatcagtggc atgtttgcaa agacggaacc gcctgcttct gccataacat gcgatacaac
    10921 ggcgggcagc cagctgaaat tcgcggaacc gacgcccagc agaatacctg cgataggcag
    10981 tacggatacc ggcagcatca gcgatttacc gaccttttgc aggttagcaa atgcattctt
    11041 aaacataatt gagagtgctc ctgagtatgg gtgctttttt tacgttctca cgcgtggcaa
    11101 ggggggagag cctcgccgtg tacagggcat ctaagcgccc tttatttatt acacagagta
    11161 aaataattca gtgccaattt gtttgacggc tatcacgttt caacttcaga tcattggaga
    11221 agttgcacat tttgacaaaa catatgtcac aatatttcaa acgtggttat tcaccgccca
    11281 attttcggcg gtgaacttta ctcgttttag tcattaatta cgagctttaa aaaaactcat
    11341 tcaacggatg gattgaaggc gggaagcgtc gatgtgaaac agacgggcga agttatcggt
    11401 ggttacctgc gccagttctt caacggcaac acctttcaac acagccatgt attctgcaac
    11461 gtcacgaacc atcgcaggtt gattctcttt tcctcgatgc ggcaccggcg caaggtaagg
    11521 tgagtcagtt tccaccagta accgatccag ggggacataa cgcgcagcat cgcgcagttg
    11581 ctccgcatta cggaaggtca caatgccgga aaaggagatg taaaatccga gatccagtaa
    11641 tttgcccgcc gtttctctgt cctctgtaaa acagtgtagt acgccaccgc aatccgtcac
    11701 tttttcttcg cgcagaattg ccagcgtatc ggcgcgggcg tcacgggtat ggacgataac
    11761 cggcttgttc agttcacgac caatctggat atgatggatg aaggactctt gctgacgtac
    11821 tttagtttcc ggtgtgtaat aataatccag cccggtttca cccagcgcta caacaccctc
    11881 ttctgccgcc agacggcgta aatcttctac atcgtaggga tcattctggt tgagtggatg
    11941 cacgccacaa gaaaatacga cgttgtcacg ttcgcctacc agatcccgca tgtgtaaata
    12001 acccggtaat gttgtggcga ctgccagaca aaatttcaca tcgcgtgcgg cggctttcgc
    12061 cagaacgtca tccacgtcct tatgcaaaga ttcataatcc agaccatcga gatggcagtg
    12121 tgagtcgact aaaaacatga tgtctctctt aaagatgagg aaccggtagc acaacgcccg
    12181 gttgcaggta atgctcaata cgcagtaaaa gatcggtgat gagaagctcg cggttgatgc
    12241 ctgtgacaga cattaactgt tcacgaatgt ggcaaacatc ccccagtata gcctgcaggc
    12301 gcgagggaga aagatggttt gccagttcgg cgaccaggcc cggcacatca acattggtca
    12361 cctgcgcagc gccatgatgg cgttttagcg catccatcag caacgttgcc agccagtgta
    12421 aacgcgccgg agcttgttca tgattaaggg ccgccagcag cgaataccag tcgcccgatg
    12481 gcacgctata tgccaacgcc tgacacaatg tttcacgagc ctgccagtta tctccctgaa
    12541 acaacgccag tgccgcgcca ggcgaaccgg cgcttaagcg caatgcggca agtaatgcat
    12601 cctgtgacat tgtcacttcg cgtgaaagcc aggtcacggc gtactgttcc ggcggcggcg
    12661 caaggtaatg taaccgacaa cgactacgta acgttgccag taaacgttca ggctcgcggg
    12721 tagccaggaa aaaccaggtt tctgctggtg gctcttcaag cgttttcagc aatgcgttag
    12781 ccgcggcgtc ggtcagtaag gcagcatcgg ttacccagac aactttcgca ccacctaagc
    12841 gtgcgtgctc attcagcttt tcggtgacct cacgtaccgc atcaatgccc agcgtatttt
    12901 tccctttttc gggagccagg gtgtagtaat cgggatgcgt gccagcctgc atcaactgac
    12961 atccacgaca gtgaccgcaa cttttgtggc cctgcggttg ttggcagagt aaataacggc
    13021 tcagggcgta gattaaagca tcatcgccca tgcccggtaa cgcctgaatg agtagcgcat
    13081 ggtgacctct tccggcctga tagctggcta ccagtttttc gaaatcaggt cgtaaccatg
    13141 gataccatct catgcgtcca actccttcac ccagcgggtc acggtagtgc ggattgcatc
    13201 catcacggcc tccagcggct gggtggcatc aatggtatga atacttttat cttgtgcagc
    13261 cagttccaga tagcgggcgc gggtgcgatt aaagaaatcg aaagactctt gctcaatacg
    13321 atccagctcg ccgcgcgcac gcgcgcgttt taagccaact tccggggtaa catcgagata
    13381 gagcgttaag tcggggcgaa aatccccgag aacagcatca cgcagtgttg ccagcatatg
    13441 ttggtcaata ccacgtcctc cgccctgata cgcctgagtg gagagatcgt ggcgatcgcc
    13501 aataacccag gtgccgttag ccagcgcagg tttgatgacc gtttctacca gttgaacgcg
    13561 cgcggcataa aacatcagaa cttcggcttt atcggtaatg atttcatcgc ctaccgattt
    13621 gatatccagc accaggcttc ttaacttttc ggcaagttgc gtaccaccag gttcccgagt
    13681 gaaaaccatg tcgcggatac ccagttgctc gagcgtctca accaccacat tacgcgcggt
    13741 agttttgcct gcgccttcca gcccctcaat gacgatatac ttactgcgca tttttttcct
    13801 taagcacttt cagataatcc tgcacagact tgttatgact ggcaagattg gtattaaacg
    13861 tgtgaccacc tttaccatcg gccacaaaat agagatacgg cgtttttgcc ggatgcgcag
    13921 cagccttcag cgaatccgcc cccggcgtag ctatcgcacc cggcggcaaa ccggtaatgg
    13981 tataggtgtt atacgctgtc ggcgtttcca ggtctgcacg agaaagtttg ccattataac
    14041 gctctcccat cccgtaaatc acggttgggt cggtctgcag gcgcatacca atgcgtaaac
    14101 ggttgataaa taccgaggca acctgatcgc gttcactggc aatggcggtt tctttttcga
    14161 taattgatgc catcgtcacc agctgatttt tatctttata aggcagaccg tccgcacgcc
    14221 cttcccaggc gctatcgacc gctttcacca ttttcttgtg cgctcgcttg agtaacgcga
    14281 catcggtggt attggcggta tacatccagg tgtctggcca gaaccaacct tcaatccact
    14341 ccgggttttc cagttcaagt gcctgcgcta cggtgatgta cttatcatcg ctcagcgtgt
    14401 gcttgatata cggggcctca cgcaattgct tgaggtagtc gctcagacgc atcccttcta
    14461 ccagtcgcag agggaactgt gcttctttac cgctttccaa caatttcagc atctcgcgca
    14521 cggtcatctg cggtgtaaag cggtaagtcc cggctttaaa gtgagaaaga tccggttcga
    14581 tacgcagcaa ccattgaaaa acccgtgggc gattgatgat cttatcggca taaagctgtt
    14641 caccaagcgc cagacgtccg gtccctggct tcagggtaaa tatcgtctct tctttgataa
    14701 gcaatttgct gtcggcaaga tagcgaacct tccagacgcc cacaccagcg gcgataccca
    14761 gtaccaccag caataacaag attatcaata acactttttt catgactaat tcgggcgctc
    14821 acaaagtggg gctaaatatt catataacgt tgctgacgaa aaggagacat cgccacaggc
    14881 acgtacgggc atcactggca ttaacgcatt acaaataacc atctcatctg cctgcaacgc
    14941 ctcttccaga gaggcttgca cttcgacaag ctgataagag gattgtgcca gcaaacggat
    15001 acagaattgt cgcataatgc cgttaacacc tgcctgatcc agtcgcggcg tataaactac
    15061 gttgcccttc cgccagaaca aattagctgc acagcattcc gtaacccccc cttcgctgtc
    15121 aaggaccagc gcctcatcag cgtttgtctg ctcaagatga gagcggatca atacttgctc
    15181 aagccgatta agatgtttaa tacctgcaag atgaggattg cgccccagcc gcaccgggct
    15241 tagcgccaac gtgatccctt cgttacgcaa acggtcgtaa tgtgcaggat aagccgtaac
    15301 ggagagaatc cgcgttgccg gtccactgtt caatgtgctg taccctcgcc cgccactacc
    15361 gcgactgatc acgactttca gtacaccatt ttgctgttct gctgccagcg ttttcatctc
    15421 ttgttcaagc tgaggccaga agtcacagga aatcatcaac cgctgacaag catcctgtag
    15481 tcgctggata tgcgccgata acaaactgac tttaccgtcg ataactctgg cggtggtaaa
    15541 acaaccatca ccaaactgcg ttgcccgatc gcttactgac agcgattcct gcttataacc
    15601 gttaattaag aacataccgg ctccttatca gagaattgtt agtgtggcag catgttcact
    15661 acggaacaag tcggaataaa agctaagaaa aaaggcccgc aagcggacct tttatatggg
    15721 tggaaaatga caacttagat ctttttaaag atcaaagaac cattagtgcc accgaagccg
    15781 aaggagttac acagcgtgta ttccattccg ctgacctgac gtgcttcatg cggtacgaaa
    15841 tccagatcgc aaccttcatc cgggttatcc aggttgatgg ttggcggaac agcctgatcg
    15901 cgcagcgcca ggatggagta gatagattct actgcacccg ccgcacctaa caggtgaccg
    15961 gtcatagatt tcgtggagct taccaacaca cggcttgcag cttcaccgaa gatggttttc
    16021 accgcctgcg cttcagcttt atcgccagcc ggcgtagaag taccgtgcgc gttaacgtag
    16081 ccaatctgac tcgcttcaat gcctgcatca cgcagagcat ttgccattgc cagagctgcg
    16141 cctgcgccat tttctggcgg tgacgtcata tgataagcat cgctgctcat accaaagccg
    16201 acgagttcag cgtaaatttt cgcaccgcgt tttttcgcgt gttcgtactc ttcaagtacc
    16261 agcataccgg caccatcgcc cagtacgaaa ccatcacgct ctttatccca cgggcggctc
    16321 gccgcttgcg ggttatcatt gcgggtagat aatgcacgtg ccgcgccaaa accaccaaca
    16381 cccagcggcg tactggcttt ctctgcgcca cctgcaacca tcacgtcagc atcgccatac
    16441 gcgataatac gcgcagcatg gccaatgttg tgcacgccgg aagtacaggc ggtcgcgata
    16501 gagatgcttg ggccacgcag gccatacatg atagtcagat gacctgccac catgttcaca
    16561 atcgttgacg gaacgaagaa tgggctgatc ttacgtggac caccgttcat cagagatgtg
    16621 tggttttctt cgatcagtcc gaggccgcca atcccggagc caattgcggc accaatgcgg
    16681 gttgcgttct cttccgttat ttcaaggcca gaatcctgca tggcctgaac gccagcgaca
    16741 attccatatt gaatgaaggc atccatcttg cgctgttctt tgcgcgagat aatgtcctca
    16801 cagttaaaat cctttactaa gccagcaaat ttcgttgcat aggcgctagt atcgaaatgg
    16861 tcgattaggc tgatgccact ctgaccggca agcagagctt tccaggtaga ctctacggta
    16921 ttgccgacag gagacaacat gcccagtccg gtcacaacta cacgacgctt agacacgttt
    16981 gtcctccagg gagggaaaaa atgattctag tgggacaaaa agataaaact caggcggtcg
    17041 aacgaccgcc tggagatgtt cacttacgcc tggtggccgt tgatgtaatc aatggcagcc
    17101 tgaacggtgg tgattttctc agcttcttcg tccggaatct cagtatcaaa ctcttcttcc
    17161 agagccatta ccagctcaac ggtgtcaaga gaatccgcgc ctaggtcttc aacgaaagaa
    17221 gcattgttgg taacttcttc ctgcttaacg cccagctgtt cgccgataat tttcttaacg
    17281 cgttcttcga tagtgctcat actcttaaat ttcctatcaa aactcgcttt cgcgatggtt
    17341 ttcgtagtgt ataaaatgtt gaaaaatttg caactaaatc ccggcaggtc ttaccacgat
    17401 tttacgttat tttgaggcct taccccccaa taacgcaaat cattttgcac taattattac
    17461 tcataaccac gcagactgcg cgcaacatga gcattttgtg caaatcgcgg tcagaccatg
    17521 tacatcccgc cgttcacatg caaagtttca cccgtgatgt aagctgcttc gtcggatgcc
    17581 aggaatgcaa ccgcgttggc gatttcctgt gcgccgccga ggcgacccgc aggaacctgc
    17641 accaggatac ccgcacgctg gtcatcgctc agcgcacgtg tcatgtccgt ttcaataaag
    17701 cccggagcaa caacgtttac agtaataccg cgtgacgcaa cttcgcgcgc cagtgattta
    17761 ctgaagccga tcaagcccgc tttcgccgca gcgtagttgg cctgaccgcc atttcccatg
    17821 gtaccaacca cagaaccgat agtgataata cgaccatgac gctttttcat catagcgcgc
    17881 attaccgctt ttgacagacg gaaaacagat gaaaggttgg tttcgataat atcgttccac
    17941 tcttcatctt tcattcgcat taacaggtta tcacgagtga taccggcatt attgaccagg
    18001 atatccactt caccaaattc tgcgcgaatt ttttccagaa cagattcgat agatgccggg
    18061 tcggtcacat tcaacatcag acctttgccg ttagcaccta aataatcact gatcgcctga
    18121 gcgccatttt cactggtcgc agtgccaata actttcgcgc cacgggctgc gagcgtttca
    18181 gcaattgcgc ggccaattcc gcggcttgca ccggttacca gtgcgatttt tccttcaaaa
    18241 ttcatgatgt tcctctttta aagctcgagc gccgctgcca tcgctgaagg ttcgttcagc
    18301 gccgaggcgg tcagggtgtc gacaatgcgt ttcgtcaggc cagtaagcac tttgcccggg
    18361 ccgacttcat agagatgttc tacgccttgc gctgccatgt actcaacaga cttcgtccac
    18421 tgaaccgggt tatacaactg acgtaccagt gcgtcacgaa tggcatcacc attggtttcg
    18481 catttcacat caacgttatt cacaacagga actgttggtg cgttaaaggt gattttcgct
    18541 aattctactg ccagtttgtc ggctgctggt ttcatcagcg cacagtgaga cggtacgctc
    18601 actggtaacg gcagagcgcg ttttgcgccc gcagctttac aggcagcgcc agcacgctca
    18661 accgcttctt tatgaccggc aataaccacc tgtcccggag agttaaagtt taccggagaa
    18721 acgacctgac cttctgcagc ttcttcacac gcttttccaa tagacgcatc atccagaccg
    18781 atgattgccg ccatagcgcc cgttccttcc ggtacggctt cttgcatgaa cttgccgcgc
    18841 atctcaacca gacgcaccgc atcagcgaaa tcaatcacac cagcgcaaac cagcgcggag
    18901 tattccccca ggctgtgacc ggccatcatt gccggtgctt taccgccatg ctgctgccat
    18961 acgcggtaca gcgcaacaga ggcagtcaac agcgctggct gggtttgcca ggttttattc
    19021 agttcttcag ctggcccctg ctgggtcagc gcccacaggt cgtagcccag cgccgcagaa
    19081 gcttcagcaa acgtttcttc gacaattgga tagctcgccg ccatatcagc cagcattcca
    19141 acggtttgag aaccctgtcc agggaacaca aatgcaaatt gcgtcatgtt ttaatcctta
    19201 tcctagaaac gaaccagcgc ggagccccag gtgaatccac cgccaaaggc ttcaagcaga
    19261 accaactgcc ccggcttgat gcgcccgtcg cgtacagctt catccagcgc gcacgggaca
    19321 gaggccgcag aggtattacc gtggcgatcc agcgtcacca cgacattgtc catagacata
    19381 ccgagttttt tcgccgttgc actgataata cgcaggttag cctgatgcgg aaccagccag
    19441 tccagttgag aacggtcaag attattcgcc gccagcgtct catcaacgat gtgcgccagt
    19501 tccgttaccg caaccttgaa gacttcgttg cccgccatcg tcagatgaat tgaattctct
    19561 ggattcacgc ggtcggcgtt tggcagcgtc agcaattcac cataactacc gtcggcatgc
    19621 agatgggtgg aaatgattcc cggctcttca gaggcagcca gcaccgcagc gcccgcgcca
    19681 tcgccaaaaa taataatagt cccacgatct gttggatcgc aggtgcgcgc cagtacatcg
    19741 gaaccgacga ccagagcata cttcaccgcc ccagatttca cgtattgatc ggctacgctt
    19801 aatgcatagg tgaaacctgc gcaggctgct gcaacgtcaa atgccgggca acctttaatg
    19861 cccaacatgc tttgaatctg acaagctgcg ctcgggaaag cgtgcgtagc agaagtcgtt
    19921 gccacaacga tcaggccaat ctggtctttc tcaatgcccg ccatctcaat tgcgcgtgtc
    19981 gccgcttcaa agcccatggt tgaaacggtt tcgtttggcg cggcaatgtg gcgttcgcgg
    20041 ataccggtac gagtgacaat ccactcgtca gaggtgtcca ccattttttc caaatcggcg
    20101 tttgtccgca cttgttcggg cagatagctg ccagtaccaa taatcttcgt atacatgtac
    20161 gctcagtcac ttttcggtta tataccgtca cttgcaaact gcgagttcgc tggcagcgtc
    20221 ctgctaccgc agagttccgc ttttgccacc gtccagcagc tcaaaaccag ctgggtatac
    20281 agattccagg cgagcggcaa ttcgctgagg aacttgtcgc tgcaccgcct gcactgcctg
    20341 ttcaatcgcg accgcaaaag ctcgctgatt ggctgcacca tgacttttta tcaccgtgcc
    20401 gcgcaatcct aacagacagg cgccgttata ctggtcgggg ttgaggtgac tgaatcgcct
    20461 cgtcaggctc ttttgtagcc aacgctttaa taacagtagc caccacgacc gttttttccc
    20521 ttcaccctga gatttcagca gagaaaggaa catcctgaca acaccttcca tcgtctttaa
    20581 tgtgacattt cctgtaaagc cgtcacaaac cagcacatct gtcttgccag ttaacaactc
    20641 attggcttca agatagccga tataattgat agaagggatt gttttaagca ccgctgaggc
    20701 atcccgaata ctatcgagac cctttacttc ttcttcacca atattgagca acgccacgcg
    20761 aggattggga atttccacca cctcttcagc caggactgag cccataatgg caaattgcac
    20821 caacattgtg ctgtcacaat cgacgttggc ccctaagtca aggaccaccg ttttgccctt
    20881 ttgctgatgt ggtaataccg tcaccagcgc cggacgctca atcccctcca ggggcttgag
    20941 taataatttt gccagcccca tcagtgcccc ggtattaccg gcactgacac aggcttgcgc
    21001 tcgaccttct ttcaccagct ccagggccac gcgcattgaa ctcccacgac tggcgcggat
    21061 agcttgcgaa ggccgggcat cactggcgat aactgactgc gcaggaataa tctgcagacg
    21121 cgaacgttgt tcaaagtcag ctttagcaag taatggcgtg atggcgtcgg gattgccgac
    21181 taaaagaaga gtgagttgcg aattagaatt cagtgcctgc aatgctgcag gcactgtcac
    21241 ggaagggcca aaatcccctc ccatgacatc taacgccagg gttagacgtg tcaaggtatc
    21301 gtcgccgccc ggtttggtct ttccccagtt gcctggggaa aatcctcact aagcttcatc
    21361 acgcagacgc gtgattactt agcgatgacc ttgcggccgc ggtagtaacc gtcggcagtg
    21421 atgtggtgac gcaggtgttt ttcaccagaa gttttgtcta cagacaggct ggtgactgcg
    21481 gtcagcgcgt catgggaacg acgcatgcca cgtttggaac gggttggttt attctgttgt
    21541 acggccatgg accttactcc tcaattacgg tttatccccg atccaacggg gagcaccaat
    21601 tacttacgct ttaagctggc taatacggca aatgggtttg gcttttgcgc ttcttcaggc
    21661 agttcaccaa agaccatgtc cgcttcggac acttcacagt gttcagaatc atgcaccgga
    21721 actaccggca aggcgaggat gatttcatct tcaaccattg caagcagatc gatttcaccg
    21781 aattcgttaa cctcaatcgg ttcatacgct tccggcagtg cttcagcctg ttcgtctgaa
    21841 cgcacaggac taaaacaata cgttgtgtag acctgatgag taaacggctt cccgcaacgc
    21901 tgacactcga gcgttaccgt caccttcgca tcgccgttta acactgcgag acgttggtta
    21961 tcgatagcga acgacatgga gcattccaca tcactgtcca cactgactac ggattcggcg
    22021 acgcgctcaa cctgatcagg agtatagata ccctggtaat caaggcgttt ttgagccgta
    22081 cgaaccggat cgagagtcag gggtaatttt accttttgca tagggcgcgc atattaactt
    22141 tgtaacgtca tagagtcaaa gaaaaaggca gcctgtggtt gccttttgcc aataattcgc
    22201 acacattgcg ggttactgct ttattttcgt tcagtgctga taacgaacat caacctgcaa
    22261 aacgaagcgc atagtttaaa atggctatca tcaatacgct atatctgatg gaaaaaaata
    22321 tgcctaaact tattttagcc tccacctcgc cctggcgtcg cgctcttctg gaaaaactgc
    22381 aaatctcttt tgaatgtgca gcacccgagg tcgacgaaac tccacacagc gacgaatcac
    22441 cgcgacagtt ggtgcttcga ctggcacaag aaaaagcgca atctctggcg tcacgttatc
    22501 cggatcattt aattattggt tcagaccagg tatgtgttct tgatggcgaa atcaccggta
    22561 aaccgttaac ggaagaaaat gcccgtctgc aattacgcaa agccagcggc aatatcgtca
    22621 ctttctatac tggactggcg ttgtttaatt cggcgaatgg gcatctgcaa acagaagtgg
    22681 agccttttga cgtccatttc cgtcacctga gcgaggcgga gattgataat tacgtgcgta
    22741 aagagcatcc cctgcactgc gcgggtagct ttaagagtga aggatttggc attactctgt
    22801 ttgagcgttt agaggggcgt gatcctaaca cgctggttgg tttaccgcta atcgcgctgt
    22861 gtcagatgtt acgtcgggaa gggaaaaacc cgctgatggg atagtttttt gcgccggatg
    22921 cggttacgtc gcatccggca atcggtgctg gcggcctgat gcggcgtaaa cgccttatcc
    22981 cattatcatg cttatatgct cgtattagcg cgcgttacgc agcttttgca aacaacgctt
    23041 caaaccatca tccatcggcg cttcgatacg catcacctca ccggtccccg gatgggtaaa
    23101 cttcaacgct gcggcgtgca ggaacagacg atttaatccc gtgcctgctt cagtgagctg
    23161 tctgtcaaat tcacggtcac cgtagcgatc gtcaaaggca atcggatgac ctgcatactg
    23221 tgtatgcaca cggatctgat gagtgcgccc tgttaccgga ctacaacgca ccagggtggc
    23281 aaatgcatag cgttcttcca ctttaaagcg tgtttccgac ggtttgcctt cctgactcac
    23341 acgcacgata cgttcgccgc tttgcagaat atttttcaat aacggcgcct gaacgctctt
    23401 cacatgcgac tgccactgac cgcgcaccag cgccaggtaa tctttttgca tccctttttc
    23461 acgtaattgc tcatgcagag aacgcaacgc cgagcgtttt ttcgctacca gcaacacacc
    23521 tgaggtgtcc cggtcaagac gatgaaccag ttcaaggaac cgcgcttccg ggcgcaacgc
    23581 ccgcaaacct tcaataacgc cgaagcttaa accactgccg ccatgtaccg ccgtaccgga
    23641 aggtttattc agcaccagga tgtgatcatc ttcatataag atgacgtccg ccagcgccgc
    23701 caccttttgc agatgtggcg aaaccgcctc ttcttcccgc tcagcaacgc gaaccggtgg
    23761 aatacgcacc tcatcacccg cttcgagttt atattcaggc ttaatacgtt ttttgttcac
    23821 ccgcacttcg cctttacgca aaatacggta aatcatactt tttggtacgc ctttcaattg
    23881 ggtacgcaaa aagttgtcga tacgttgccc cgcttcgtcg gcggtgatag caacaatttt
    23941 tacggatgga gtctctgttt tcatggtggg cgattctaat tagccaacag gattcgcgcc
    24001 actcattttt ctatgcttat atttagtttt gcaccttatt acttcactac gtgatcactt
    24061 tattgatggt tattaaacca atcaccagca agaagtgaaa aaactgtgag taagcgggtg
    24121 ataaatggta aaagtcatct tgctataaca aggcttgcag tggaataatg aggccgtttc
    24181 cgtgtccatc cttgttaaaa caagaaattt tacggaataa cccattttgc ccgaccgatc
    24241 atccacgcag caatggcgta agacgtattg atctttcagg cagttagcgg gctgcgggtt
    24301 gcagtcctta ccggtagatg gaaatatttc tggagagtaa tacccagtct gtttctttga
    24361 taattgcgct gtttttccgc atgaaaaacg ggcaaccgac actctgcgcc tctttgagct
    24421 gacgataacc gtgaggttgg cgacgcgact agacacgagg ccatcggttc acacccggaa
    24481 aggcgttact ttgcccgcag cttagtcgtc aatgtaagaa taatgagtaa gttacgatga
    24541 aaagaatgtt aatcaacgca actcagcagg aagagttgcg cgttgccctt gtagatgggc
    24601 agcgtctgta tgacctggat atcgaaagtc cagggcacga gcagaaaaag gcaaacatct
    24661 acaaaggtaa aatcacccgc attgaaccga gtctggaagc tgcttttgtt gattacggcg
    24721 ctgaacgtca cggtttcctc ccactaaaag aaattgcccg cgaatatttc cctgctaact
    24781 acagtgctca tggtcgtccc aacattaaag atgtgttgcg tgaaggtcag gaagtcattg
    24841 ttcagatcga taaagaagag cgcggcaaca aaggcgcggc attaaccacc tttatcagtc
    24901 tggcgggtag ctatctggtt ctgatgccga acaacccgcg cgcgggtggc atttctcgcc
    24961 gtatcgaagg cgacgaccgt accgaattaa aagaagcact ggcaagcctt gaactgccgg
    25021 aaggcatggg gcttatcgtg cgcaccgctg gcgtcggcaa atctgctgag gcgctgcaat
    25081 gggatttaag cttccgtctg aaacactggg aagccatcaa aaaagccgct gaaagccgcc
    25141 cggccccgtt cctgattcat caggagagca acgtaatcgt tcgcgcattc cgcgattact
    25201 tacgtcagga catcggcgaa atccttatcg ataacccgaa agtgctcgaa ctggcacgtc
    25261 agcatatcgc tgcattaggt cgcccggatt tcagcagcaa aatcaaactg tacaccggcg
    25321 agatcccgct gttcagccac taccagatcg agtcgcagat cgagtccgcc ttccagcgtg
    25381 aagtccgtct gccatccggt ggttcgattg ttatcgacag caccgaagcg ttaacggcca
    25441 tcgacatcaa ctccgcacgc gcgacccgcg gcggcgatat cgaagaaacc gcgtttaaca
    25501 ctaacctcga agctgccgat gagattgctc gtcagctgcg cctgcgtgac ctcggcggcc
    25561 tgattgttat cgacttcatc gacatgacgc cagtacgcca ccagcgtgcg gtagaaaacc
    25621 gtctgcgtga agcggtgcgt caggaccgtg cgcgtattca aatcagccat atttctcgct
    25681 ttggcctgct ggaaatgtcc cgccagcgcc tgagcccatc actgggtgaa tccagtcatc
    25741 acgtttgtcc gcgttgttct ggtactggca ccgtgcgtga caacgaatcg ctgtcgctct
    25801 ctattctgcg tctgatcgaa gaagaagcgc tgaaagagaa cacccaggaa gttcacgcca
    25861 ttgttcctgt gccaatcgct tcttacctgc tgaatgaaaa acgttctgcg gtaaatgcca
    25921 ttgaaactcg tcaggacggt gtgcgctgtg taattgtgcc aaacgatcag atggaaaccc
    25981 cgcactacca cgtgctgcgc gtgcgtaaag gggaagaaac cccaacctta agctacatgc
    26041 tgccgaagct gcatgaagaa gcgatggcgc tgccgtctga agaagagttc gctgaacgta
    26101 agcgtccgga acaacctgcg ctggcaacct ttgccatgcc ggatgtgccg ccagcgccaa
    26161 cgccagctga acctgccgcg cctgttgtag ccccagcacc gaaagctgca ccggcaacac
    26221 cagcaactcc tgcacaacct gggctgttga gccgcttctt cggcgcactg aaagcgctgt
    26281 tcagcggtgg tgaagaaacc aaaccgaccg agcaaccagc accgaaagca gaagcgaaac
    26341 cggaacgtca acaggatcgt cgcaagcctc gtcagaacaa ccgccgtgac cgtaatgagc
    26401 gccgcgacac ccgtagtgaa cgtactgaag gcagcgataa tcgcgaagaa aaccgtcgta
    26461 atcgtcgcca agcacagcag cagactgccg agacgcgtga gggccgtcag caggctgagg
    26521 taacggaaaa agcgcgtacc gccgacgagc agcaagcgcc gcgtcgtgaa cgtagccgcc
    26581 gccgtaacga tgataaacgt caggcgcaac aagaagcgaa ggcgctgaat gttgaagagc
    26641 aatctgttca ggaaaccgaa caggaagaac gtgtacgtcc ggttcagccg cgtcgtaaac
    26701 agcgtcagct caatcagaaa gtgcgttacg agcaaagcgt agccgaagaa acggtagtcg
    26761 caccggtagc tgaagaaact gtcgctgccg aaccaattgt tcaggaagcg ccagctccac
    26821 gcacagaact ggtgaaagtc ccgctgccag tcgtagcgca aactgcacca gaacagcaag
    26881 aagagaacaa tgctgataac cgtgacaacg gtggcatgcc gcgtcgttct cgccgctcgc
    26941 ctcgtcacct gcgcgtgagt ggtcagcgtc gtcgtcgcta tcgtgacgag cgttatccaa
    27001 cccaatcgcc aatgccgttg accgtagcgt gcgcgtctcc ggaactggcc tctggcaaag
    27061 tctggatccg ctatccgatt gtacgtccac aagatgtaca ggttgaagag cagcgcgaac
    27121 aggaagaagt gcaagtgcag ccgatggtga ctgaggtccc tgtcgccgcc gctgtcgaac
    27181 cggttgttag cgcgccagtt gttgaagaaa tggctgaagt cgtagaagcc cccgtaccgg
    27241 ttgccgaacc gcaaccggaa gtggttgaaa cgacgcatcc tgaagtgatt gccgccgcgg
    27301 taactgaaca gccgcaggtg attaccgagt ctgatgttgc cgtagcccag gaagttgcag
    27361 aacacgcaga accggtggtt gaaccgcagg aagagacggc agatattgaa gaagttgccg
    27421 aaactgctga ggttgtggtt gctgaacctg aagttgttgc tcaacctgcc gcgccagtcg
    27481 tcgctgaagt cgcagcagaa gttgaaacgg taaccgcggt taaaccagag atcaccgttg
    27541 agcataacca cgctaccgcg ccaatgacgc gcgctccggc accggaatat gttccggagg
    27601 caccgcgtca cagtgactgg cagcgcccta cttttgcctt cgaaggtaaa ggtgccgcag
    27661 gtggtcatac ggcaacgcat catgcctctg ccgctcctgc gcgtccgcaa cctgttgagt
    27721 aataattagc tcaagtaatc aagccctggt aactgccagg gcttttttat ttcatctttg
    27781 aatcaacata ttaaaattca tcgaatggca tccttgctaa ccaacaatgc aaaataggca
    27841 aaaaaatgcc cgccccggcg atccctgagc gggcagacaa aacataccca gtttcatgat
    27901 atgttttaaa gcgagcttat ttgttgagct ggaagagcga caatccctgc atatcggtaa
    27961 atgctttata cgatgcctgc aatgccgttt gctgcatgat gtaagatgaa atagttgcat
    28021 tccagtccac atcaaccaga tcgctcatct gctgcgtttg ccctaatgcg cgatcgctac
    28081 ctaatgaatc cagcgactcc agttcgttca gctgcgtgcc taattccgcg cgaacggtca
    28141 ggacattgtt cagcgagttt ttcagtccgc ggttggtttt atctaacgcc gcagcggcgg
    28201 tttctttatc cgcttcgcta tccgcgaccg gcgttttcag ggctgcgatg gcgctatcca
    28261 gcatggcaaa aagattggtt tcagaagcgc taccgtctgg ttccgctacc gcgttactgg
    28321 taatactgtc gaaaattttg tcgcccgtat gtcctatcac catcgaacgc gaagcatcga
    28381 cctgttgctt aatactttct gttccgccga cataatcacc gttaacctca ctaaacggcg
    28441 cagtctctgt tttataaccg gcaaaaatgt agcgcccgtt accgtcagtg gtgttcgcca
    28501 gattcagcaa ctggtcacga agcccctgaa tatccgtagc cagcgaggcc cggtcattgt
    28561 cactcaaggt gccattgctg gcgtagacaa ttttttcctg agcattctgg atagcggtgg
    28621 tgacctggct aagtacactc tcttccagtg acactttttg agtggcgaaa gtacgtgcca
    28681 gcgtgtactg gctgttttgc gcctgtgcct gggagagaac aacagcttgt gatgcagcaa
    28741 tgggatcgtc tgaagggtta acgactcgct tacccgtcga catctgctcg ccgtacttca
    28801 tccattctgc ctgagaattg gtgatgccgc gcatgttttg ctggtacatc atctgggtac
    28861 tgaaacgcat tttatctccc cttagcgaat gttaatcagc gcatcaaaaa tcgcgtttgc
    28921 cgtctgcaga acctgcgcat tcgccaggta atactgctga aaacgttgca gatttccgta
    28981 ctcctcatcg agattgacac cggaaatcga ctgctgctga ttggaaagct gcgtcaccac
    29041 attaccttgc gtggtgctac tggttttcaa cgtcgcggtt ttattaccga tatcactcac
    29101 taacgaggca taagcgtcgt taaaggattt cgctccgccc accgttttac tgttgctttg
    29161 cagatccagc agggcctgac cgttgcggtt gtcgctatca cccgcatctt cttcgctcgc
    29221 catcgctatt ttcgcttcgt cggtgattaa tacatccatg ttgacgatgg cgtcacttac
    29281 tggtttcagc gtgaagctgt cgttaacggc aggcgttcct gtaaacgtca actccagacc
    29341 atcaaatgcc actttaccgt tggcatctgg cgtcaccgta aaagtggtat tgctggcaag
    29401 gcgggtgacc tgccactgat tattatcgaa cgagattttg taatctgtcg ccagtaccgc
    29461 ggaggcatca gttaccgtgg caccgatcgc aacgttaccg ttgtttttag tgttttgcag
    29521 aaccgcgggc ttaccgatag caaagaaatc ttcaccctca tcgccgttgg catcaaatcc
    29581 ggctttgtgt tggctgttga aagcctcagc aaatgccagc gccagttgtc caagcgtatt
    29641 acgcgtctgg tccagatcct gagaacggaa tgtcagaatg ccgcccagcg acccggtatt
    29701 cagtaatttc tccgggatct caatattgcc tgccgtccca tcaacataag cgacagtcgt
    29761 acgagaaggg tcggcgctgg aaggaactgc cgccagttgc cgcgccgtac ttccctgaac
    29821 cagtgagtaa ccattggcca tcgtgatgtt ataagtgccg ccatcctgaa cgctgacttc
    29881 tacaccaaca atctggttta attcgctcac cagttgatcg cgttgatcca gcagattgtt
    29941 aggtgacgcc cctgccccca cgcctgtcag gcgggagatt tgatcgttca ggctggcgat
    30001 ttgtttagcg taattgttga tctgatcaac gctggcacct atcgcgatat tgacctgttt
    30061 gtcctggtcg cgaagatact gatcggtggt tttaaactga ttcaccaacc cttctgattt
    30121 cccaatcagc gcctggcgcg ctgccgggtc ttccgcgtta ctcaccagcg tttgcaggct
    30181 ggtgaagaaa tcctgcatct gtgttgccag cgaagaggta ctggtggaga gcatattgtc
    30241 gattttcgac atctgctcat agcgggcagt caggccgcta ctttgcgtct gcgccgcacg
    30301 taactggttg gtaataaacg catcatactc acgctgcaca ccagaaacgt agacaccatt
    30361 accaacccag ccgccagcgc ccaacgtgct attggcctgc gccataatag tggtttggcg
    30421 ggtatatccg gcaacgttat agctggagat attattactt gccgtattta acgccgcctg
    30481 ggccgcgttc agtccgctca tggcgttatt gatcaagctg gacatggagg ttccttataa
    30541 gccttcaatt acagagtatt atcggcagcg acccgccgga cttgagttat tcagaacaga
    30601 ttatcaatgt tcatgctgta ggttttgctc accttgtcgc ttatcgattt catctgctga
    30661 atcatgttgg tgagtttacg ggcatagtga ggatcggtgg catagcccgc gtcctgtagg
    30721 gcctgcgccc cctgctccgc actcgcggcg gtcgtcacgg cggcgtagcg cgggttacgt
    30781 gttaacagcc caacgtaatc cgacaatgct tccagatacg agctgtagac ccgaaacttc
    30841 gcttttactt tcttcgcttc gccattttca tattcagtcg tggtgatttc agtgactggc
    30901 cctttccagt tgccagaggc tttgacacca aacaggttat agctcggctc gccgttttcg
    30961 cggcggattt gccgttgccc ccagccagat tccagcgccg cctgagcgag gatcaaatga
    31021 tgtggcacac cgctttgctg gcttgccagt tgggcgggca gcgagagttg cgcgaggaat
    31081 gctttactgt cacctggcag cgaatcatcg tagttacgtg gcacggcctt ttgcaccagc
    31141 tgcgaaagcg cctgattttg ataacgcacc acggtttcga gcgggaattt catcggtgct
    31201 gctggcgtgg actcctctgg caatggttgt tctggcgtca tctgcttaac catcatctct
    31261 gcaagcccca gacctttgcc cgtcgtcatc tgttgggcaa tctgctggtc atacatactg
    31321 gtatacaggc gagtgtgctc gctgctgaac aggccatctt ttggtaacgc gtcgcgcatg
    31381 cttttcaaca tcatctgcac gaacatccct tccacctgac gggccaccgg acggatattt
    31441 gccgccggat cttcgctcgc tttcgccttt agttcgttga gtgattgcgc atcccaggcc
    31501 gcacttgcca gtagtttgct gtcgctgatc atcagatgat ttccagtttt gcccgcagac
    31561 atcccgcact ttgcattgat tgcaaaatag acatcagatc catcggcgta gcgcccagcg
    31621 cattgagcgc gcgcaccacg ttattgaggc tggcgctgga acgtacgctt tgcagcgaac
    31681 cgccgctctg gcgtaagtcg atctgcgttt gtggcgttac cacggtctgt ccgccaccaa
    31741 acggtgtatc tggttggctg acattggcct gacgattgac tgttactgag agatttccct
    31801 gcgctatcgc gcagctgtcg agagtcactt cgcgattcat caccaccgaa ccggtgcgcg
    31861 agttaatcac tactttagcg tcctgcgggg tgacattaac ctgcatattc tggatatcgg
    31921 caaggaaacg gacctgggaa ctgttgccac tcggtacgcg cacctgaata gtccgcgcat
    31981 ccaacgcggt ggcgctgcca tatccacgca cgcggttgat ggtgtcagcg atttgctgcg
    32041 ccatgctgaa atcttcgtcg ttaagttgca aattaagggt attcccgacg ccaaactggc
    32101 tgggtaattc acgttcaata actgcaccat tggtgatccg tccaccgttc agttggttca
    32161 cctgaacact gctaccgcca gcggaggctc ctgcgccgcc aaccagaata ttgccctgcg
    32221 ccagcgcata cacctgactg tcaacgccct taagcggagt catcaacaat gtgccgccac
    32281 gcaggctttt ggcatttccc atggaagaaa ccaccacgtc aatggtttgc ccctgacgtc
    32341 caaacggtgg aagtgacgcc gtcaccatta ccgcagcaac gttttttagc tgcatattgg
    32401 tgcccgtcgg aacggtaatt cccagctgtg agagcatgtt attaagcgtt tgtgtggtaa
    32461 acggcgtctg ggttgtctgg tcaccggtgc catccagccc caccaccaga ccatagccaa
    32521 tcagtgagtt ttgccttacc ccctgaacac tggtgagatc gcgaatacgc tcagcctgag
    32581 ccgccgtcgt aaccagtaga agaattaatg cagagagaaa tttaatcacc acagcctcac
    32641 ttacattggc gacaggttaa ggaagaaacg ctgcaaccag cccatatttt gcgcttcgtt
    32701 aatgtagcca ttgcctacgt attcaatgcg cgcatccgcc acctgagtag acggtacggt
    32761 attgctgccg ctgatagtgc gtggattaac cacgccagag aagcgaataa attcggtccc
    32821 ctgattaatg gcaatctgtt tttcacccac cacatgcagg ttgccgttga ccagtacctg
    32881 gtcaaccgtc accgtcaacg tgccgctaaa ggtattactg gcattggccc cgccttttcc
    32941 gttgaacgtg ttaccaccgg aggcttcgac atcggcacga gcgttaccaa acagcccctg
    33001 caaatagcgc ggcacagtat caaagccaaa attagtttta ccgtcacggc tggcattcgc
    33061 agaggagctt ttgctggcgc taacgttctc ctgcaacacg atggtcagcg tatcgccaat
    33121 attgcgtggt cgacgatctt caaacagcgg ttgatagcca tagttaatcg gctgagcaga
    33181 ctggaaaata gaaccgttgg cgacgggcgt cggaccggga accggttgtg cactggtcgc
    33241 cccctgcacc agcggcgtgg agggtatcca ggcgcagccg gttagtgaaa gcaccaacaa
    33301 gctggaaatg gcataagtat gcgcagcgtt tttttgcatg gctatcatct tcaaaaatca
    33361 ataaaccggt gaacctgcca ccggttatgc cttagagttg cgtcagtttt tgcagcatct
    33421 gatcggtggt ggacaccgct ttactgttga tttcgtaagc gcgttgcacc tgaatcatat
    33481 tgaccagttc ttccgccacg ttgacgttag acgtttcaac atacccttga tacagcagtc
    33541 ccgcgccgtt caggcccggc gtgctttcgt tcggtgcacc ggaggattgc gtttcggtgt
    33601 agaggttttc gccaatgctc tccagcccgg tgtcattcat aaaggtggtg agattgagct
    33661 gcccaacctg aaccggagct gcctggcctt gttgggttac gctgaccacg ccatcacgac
    33721 cgatggtgat acttaacgca ttcgccggaa tggtgatcgc tggctgcacc tgaaaaccac
    33781 cagccgtcac cagctgcccg ttctgatcca cctggaaaga gccgtcacgg gtataggctg
    33841 atgagccatc tggcaacatc acctggaaaa agccctgccc tttaatcgcg acatctttgc
    33901 tgttgttggt ctgcgacagg ttgccttggc tgtgtaagcg ttcagtggcg accgggcgta
    33961 cccccgtgcc gatttgtaat ccggagggta aggtggtttg ttcggaagac tgtgcccccg
    34021 gctggcgaat ggtttgataa agcagatctt caaacaccgc gcgctgacgc ttaaaaccgt
    34081 tagtactgac gtttgccagg ttgttggcaa tgacgtccat attggtttgc tgggcgtcaa
    34141 ggcccgtttt ggcgatccat aatgaactga tcataaaatg tcctgtagtg gattgctgac
    34201 aagcagagca gaagcacgcg gcggtgcgaa tttcccctca ccctaaccct ctccccaaag
    34261 gggcgaggga acagtccgct cgcttttatt gcaatgtcat caatcgttca attaactcat
    34321 cgacagcagt tggttggcac ggcctgcgtt atcatcgacg ctgctgatca ccttcatctg
    34381 catttcaaaa cgccgcgcgc tggcaatcat gtcgctcatt gccgcaacgg cattgacgtt
    34441 actgccttcc agaacccccg acatcacacg caaggttgga tctgcctgca gtaccggtcc
    34501 acgcgtggcc tgggtttctg cgcttaaacg aaaaatgccg tcgtcaccgc gctgcacttc
    34561 gctgcccgtg gctttcacca gtttcagacg ccccactggc gcaaccgtat ttgccggatc
    34621 gcccggattc agcgccgaga ttgttccatc ggcagctatc gtgatttccg ccccttccgg
    34681 cacagcaatt ggccccgcct cgcctatcac cggatgcccc tgaattgtca gttgcccggt
    34741 gggatcaacc tgaatgctgc cattacgcgt atacccttcg ctgccgtcag cggtctgcac
    34801 ggccaaccag ccatcctgct gcaacgcaac gtccagcggg cgcgaggtgt aatccatctt
    34861 gccgggcgtt atgtctgcac ccggtgttga cgccgttacc agcgtgcgcg tgggcagtga
    34921 aagcccttcc accggcactg cgcgcaaggc gtttaactgc gcgcgaaaac cgggcgtaga
    34981 ggcattggcc agattactgg cggttaccgc ctgttgattc agcgtctggc ttgctgctcc
    35041 catcgcggta taaattgcgt gatccattga gctatcccgt cagcgattag cgtaagttaa
    35101 ccagcgtgtt gaggatctgg tcctgggttt tgatggtctg ggcgttagac tgatagttac
    35161 gctgggcaac gatcatattg accagttctt tactgagatc gacgttggac gcttccaacg
    35221 caccgttggt cagggtgcca aagttgcccg tcccggctgt ccccaacagc gccacgccag
    35281 aagattgcgt cgcagaccag acgttgtcgc cttcggatgc cagaccttcg ttgttggcaa
    35341 agttcgccag tacaatctgc cccagcagtt gggtttgttc gttggaatag ttgccgacaa
    35401 ccgtaccgtc atcattgatt tgataactca ccagatcgcc cggtttgtag ccattctggg
    35461 tggttgccac aatgttgtta gcgccggtat tttgctgcat ggagttgagg aagctcaggc
    35521 taaacgtggc gggttctgcg ccgttaattg cgccggtggt aatattctct gttggatttg
    35581 aggtcagaac gccattggca ttaaacacca gcttcattgc aggctcggct gtacctgttg
    35641 gatcactgct atcctgggtg tagacctgcc agttattatc cccggtcttc acaaagtaga
    35701 cgctcatgtc atgagcatta ccctgactgt cgaaaacagt caccgaacct tttttgttat
    35761 agctatccgc attgctggca tcaaatgcgt taacagaggg aagcggatcg ctggaattca
    35821 ggttgatctg catcgacgcc gtggtggtag ttttcgctgc catcagggta ttcgggatcg
    35881 aaatgttagt cggattcgcc ccttgctgaa tagtcggcgg cgtaccggtt gccgggtaac
    35941 ccgtcagctg taaaccttgc atattcacca ggttacgatt ttcatccagc ttaaattgtc
    36001 cgttacggct gtagaacacc gaaccgttgc tatctaccag acggaaaaaa ccgttctggc
    36061 tgatagcaac gtccagacca cgcccggtgt tggtggtcgt gccatcggta aagtcctgag
    36121 tgataccggc aacttttacc cccagtccca ctttcgaacc ggcaaacata tcggcaaaag
    36181 aggccgtgcc tgatttaaag ccgtaggtgg cggagttggc gatattgttg ccaataacat
    36241 cgaggttggt ggcggcagcg tttaatccgc taaccgcttg agaaaaggcc atgactgact
    36301 cctgaaatgt gaaggcttaa attatctgcc gtacttcgtc gagggtggtg gtgccgtaag
    36361 tgccgagatc cagcgtatta ccgttgttgc cgcggatcac accctgcacc agagcaaact
    36421 gcagcggctg ggcaaccagt tgtgtaccac cgttactggc gctaatcgct acgttgtaag
    36481 aaccgttcgg cgcagtgctg ccgtcagtca acgtaccgtc ccaggtgaaa ctgtgaactc
    36541 cggcggtcag ttcaccaata tcaatggtgc gcacaaccgc gccattttta tcggtgatgg
    36601 tggccgtaac tttgtctgcc gcctgttgca gctcaacacc aaacggcgtg gtcgtggtca
    36661 cagccccttc ttcactgccg gttcccgcaa gaacagtggt gccggggatc atcacgccgt
    36721 gaccgatcag gttactggcc tgtaacgact ggctgttatc aatctgtccg gaaatagatc
    36781 cgagcgtggt attgagtttt tcaatcccgc tgaccgtgct gatttgtgcc aattgcgacg
    36841 tcagctcgtt gttttccatt ggattggtcg ggtcctggtt tttcagctgc gccaccaaca
    36901 aagtcagaaa actgctttgt aaatctgcgg cgttgctgcc cgtgagcgaa ctactgctgg
    36961 tggtactgac gccggtattt gtcggatcgg tggtggttac cgcaatggac atagctttct
    37021 cctttattga ccgagcgtaa gggttttcag catcatgctt ttcaccgtgt tgagcacttc
    37081 aacattggcc tgatagctgc gtgacgccga catggtgtta accatctctc cgacaacatc
    37141 aacgttcggc atttttacgt agccctttgc atccgccagc ggattacccg gttcataaac
    37201 cagtttgtcc ggggcctgac tttctataac atcggcaacc tttacgccgc ctgtcgcagc
    37261 acctggtgca gcgttaacct ggaataccac ctgttttgcc cgatatggct gtccatcagg
    37321 accggtcacg ctatcagcat tcgccagatt actggccgcc acgttcaggc gctgggactg
    37381 ggcagttaac gccgacccgg cgatatcaaa aatattcagc agtgccatcc gttaatttcc
    37441 gctctgtaaa acgttcatca tgcctttgat ttgcccgctc aacgcgctaa ggctcatctg
    37501 gtattgcagg ctgttatcgg caaactgggt gcgttcgcga tccatatcga cggtattacc
    37561 gtcaagcgaa ggctggtccg gaatacggta ttgcagttct gcggtaggag gcgtcagcgc
    37621 ctgcgccgga atgtgttgcg ttgaggtcat cgtcagagca accacactgg ttgcattccg
    37681 tccacgttgc atgacttttt taagttcact ggcaaaatcg atatcgcgcg cctgataacc
    37741 aggggtatcg gcattggcga tgtttgctgc cagcacttcc tgacgctggg cgcgcagatt
    37801 gagcgcctct tgttgaaaac gtaaggcggc gtcgagctta tcgagcatat ctcctccgca
    37861 ggtatcaaaa ttctgccatc agcttaaatg cctttacgcg cgcgttatcg gcggaataaa
    37921 cgcaaaatgg gtcgctattt atgccgttga tggtcattgt ggacaggtac aattcacgtt
    37981 gtagaaatgg ctgggggcga aaatgctggc aataaaacgt agcgtggcga tcatcgcgat
    38041 actgttcagt ccgttaagtg cggcgagcaa tctcacatcg caattgcaaa ccttttttag
    38101 ctcccagctc gcagggataa gtgatgaggt tcgtgtttct attcgtacag cgcccaatct
    38161 actaccgccg tgcgagcagc cattgctttc gatgagcaat aattcccgct tgtggggcaa
    38221 tgtgaatgtg ttggcacgct gcggtaacga caaacgatat ttacaggtta atgtgcaggc
    38281 cacaggaaat tatgtggttg ccgcgatgcc cattgtgcga ggaggaaagc tgggagctgg
    38341 caatgtcaaa ctgaaacgcg gacggctgga taccttgcca ccgcgtacgg tgctggatat
    38401 caatcaactt gttgatgccg ttagcctgcg cgatctatca cccgatcaac ctatccagtt
    38461 aacccagttt cgccaggcat ggcgggtaaa agcgggacaa cgcgtcaacg tgatcgccag
    38521 cggtgatggg tttagcgcca acgcagaagg tcaggcgcta aacaatgcag ccgtcgcaca
    38581 gaatgcgcgg gtgcgcatgg tatctggaca ggtagtcagc ggcgttgttg atgcagatgg
    38641 gaatattctt ataaacctgt aagctgttaa agattacccg tcccttgccg ataaataagc
    38701 aacacatgat aaaagcgccc tcaatgagga ataaaccatg agtattgatc gcacttcgcc
    38761 tctgaagccc gtaagcacca ttcaaccgcg cgaaaccact gacgcgccgg taacgaacac
    38821 ccgagcggca aaaacaacag cctccaccag caccagtgtg acgttaagcg acgcgcaagc
    38881 aaaactgatg caacccggca gcagtgatat caatcttgaa cgtgtcgaag cgttaaaact
    38941 ggcgattcgt aacggtgaac taaaaatgga caccggcaaa attgccgatg cgctgatcaa
    39001 cgaagcgcag caagacttgc agagtaatta atcgtatgac gcgtcttgca gagatcctcg
    39061 accagatgtc cgctgtgctt aacgatctca aaacggtaat ggatcaagag cagcaacatc
    39121 tctctatggg gcagatcaac ggcagccagt tgcaatggat tacagaacaa aaaagctcac
    39181 tgctggcaac gttggattac ctcgaacagt tacgcaggaa agaacccaat acagcaaata
    39241 gcgttgatat tagtcaacgc tggcaggaaa ttgctgtgaa aacgcagcaa ctacgccaaa
    39301 tgaatcaaca taacggctgg ttactggaag gacagattga gcgcaatcaa caggcgctgg
    39361 aaatgttgaa accgcatcag gaaccgacgc tatatggggc gaacggtcag acctcaacaa
    39421 cccatcgcgg cagtaaaaag atttcgatct gaagattatc tccggcctgc actgcaggcc
    39481 ggaatgcatt gttacaccgt ccggcgggca aattctttaa ctttgaagcc cagtaccgcc
    39541 agtgcagcga agtacgcggc aatccccgcc agcacgaccg ccattaaacg cagcaaacgc
    39601 cagggcatgg tacccaatga ccactccggc atgatatgta acatacctaa aagcacgcca
    39661 gacatcacca gtaccgccac caccagacgc aacagaaacg ccatccagcc aggttgcggg
    39721 gtaaagattt tctgcttacg caactgccag taaagcagcg aagcattcag acacgccgcc
    39781 agaccaatag aaagtgacag cccggcatgt ttcaacggac cgataaacgc caggttcatc
    39841 aattgcgtta aaatcagcgt aacgatggca attttcactg gcgttttaat gtcctggcgg
    39901 gaataaaagc caggagccaa cactttcact acaatcaggc caatcaaacc caccgagtag
    39961 gcaattaacg cccgctgggt catcagcgca tcaaaggcgg taaatttacc gtactggaac
    40021 agcgaaacgg ttaacggacc ggaaagaatg cccaacgcaa ccgcactcgg caacgccaac
    40081 aggaaacaaa gacgcagtcc ccagtccatc aaacggttgt attcatcatg attgccactg
    40141 gcaaaacttt tcgacagcga cggtagcaaa atggtgccaa gcgccacgcc cagcacaccg
    40201 gacggaaact ccattaagcg gtcggcgtaa tacatccatg acaccgaacc ggaagcaaga
    40261 aacgaggcaa aaatggtgtt gatgattaag gagatctggc tcacagagac gccaaggatt
    40321 gccggtccca tctgtttcac cacacgcatt gctccggcat cgtggaagtt aatgcgtggc
    40381 aaaaccagca tgccgatctt cttcagatgc ggtagctgat acaccagctg caggacgccg
    40441 ccgaccgtca cagcccacgc cagcgccagc accggtgggt taaagtacgg tgcggcaaac
    40501 agcgcgaaac caatcatgct gatgttaagc agtgttggag caaacgccgg aatcgagaag
    40561 cggttccacg tattcagaat cgctcccacc agcgacgcca gggagatcag caagatatag
    40621 ggaaaggtaa tctttagtag ctggctggtc agggcaaatt tgtcagctgt gtcagcgaag
    40681 cctggcgcgg tcaccatgat cacccacggt gcggcgagca tgccagcgac cgtcacaacc
    40741 gccagcgcaa gtgtcagcag gccagaaaca taagagacaa agacccgcgt ggcgtcttca
    40801 ccctgcttac ttttatattc cgccagaatc ggtacaaatg cctgggaaaa tgccccttcg
    40861 gcaaagatac ggcgtaacaa gttaggaagt ttaaaagcga cgaaaaaggc gtcggttgcc
    40921 atccctgcgc caaagattct ggcgacaatt gcgtctcgtg cgaagccaag cacacgcgaa
    40981 aacatggtca tcgagctgac ggcggccagc gattttaata aattcatcgg tgttctaatc
    41041 cagacccacg ggcaaaacgc ctgcaaggca ggcgctgaaa gaggcgaata gtctacctgg
    41101 attatggtga attgctaccg ccagatgtta cagggtttat tcactcatcg catcgcgcca
    41161 gatcttgtca acgatacgtt gcgccagcac tgcctgttcg ccggcggttt gcggaactgt
    41221 ctgattttgc acgcattcaa tgaagtgccg cgcacagccg acaaatccac gttgctcaag
    41281 cgtgctctgc caaccaggaa tcggtttatt cactacaccc tgcccacgct cctcacgcca
    41341 ttcgcgcatt tccgtaatgt cgatgagcgc accgtcagtc acggcctgca cggtttcacg
    41401 ctgacttccg gcacggcgat gcatacaagt ggtgatctgc aaaggaccgg ccgaaaaatg
    41461 gtgctcggca aacagcattt cgccagcgtc gttagtcagt agcgtaccgc catccagaga
    41521 ggctttgccg cccgacaacc acagcgcggt atccaccaca tgcagataat catccagcaa
    41581 cgtgaaataa agatcgtgtg gcccgacgct attgctacga tgtttatcca ttcttagcga
    41641 ggctgcggtg gcgagttgcg tttttaactc acagtagagt ggtgcgaaac gacggttaaa
    41701 accgaccatc aacgtcagtt ttttccgcgc cgccagttcc accagccgtt cagcatcgcg
    41761 cagattttct gccagcggtt tatcgacaca gacatgtacc cccgcattga gtaacgtact
    41821 gaccacgtca aagtgactgg cggtgctgga atgtacaaaa accgcatcgc aactggcggc
    41881 aaggctggat aacgaatcgg cataaggaat gcgccagctt tcacaaattg gcagggcttt
    41941 cgcgcgcgta ggcgaccagg ctccttgtaa cgtccagtca gacgctgccg ccagcaccgg
    42001 taaccacgct ttttgcgcaa tgccgcctaa tcccactacg ccgatacgta attttttcac
    42061 tttaatctcc caggtgggcc agcaacgaat caagacgctg tttcagttct gccacttcga
    42121 tttccagggc ttcgacgcgg gcctgtaaat caccgtcaac cgcgttcgac atatccgtta
    42181 ccgccggctg atcctctacc tcaccactga aaagatgcat gtagcggctt tcgcgtttac
    42241 ccggttcgcg ggccagacgc accacaaaag gaccatcttc gcgattcgcc agttgttcca
    42301 gcgtcaactc cacttccgcc atatcgctga attcatacat tcgcgcggcg cggctgcgca
    42361 gttcacccgg cgtctgggca ccacgcaata acaacgtggt gattaacgcc acttctgcgg
    42421 cgctcagttt cagatcgcca aattctgaat tacaaaaacg ttgctcatat ttggtgaccc
    42481 gattaccaaa accgctcact gtgcgtagat aatgacgttt gaccagatta tccagctgtt
    42541 cctgcacttc agattcactc agattcatga ccggttcacg gttcgttttc tgattacagg
    42601 ccgtgactac accattgact gagagcgggt attgctccgg cgtggtcacc tgcttttcca
    42661 gcaagcaacc aatcacacga gcttccaggg cagtaagttg atatttcatg agattctcct
    42721 tagcggccgg gcgtccagtc tggggtagtt aatgccgtca gtacgtgatc gcgccattgt
    42781 ccatcaatca acagatagtc tttcgcatag ccttcttttt caaaacccag tcgcgccagt
    42841 aaatcaccgc tgcgtttatt gtgcggcata taattagcca taatgcgatg aatatgttgg
    42901 gtgcgctgca tataacgaat ggctgcggtc agggcttcaa acatgagtcc tttgccctgc
    42961 catttttgcc caatcgaata accgagatag caggcatgaa aagagccgcg aacaacattg
    43021 gaaaaattgg caacgccaat aatctctttt tcgtccgggt cgaataagcc aaagtagaaa
    43081 gctgaacctt gtttatgaaa ttcgttaatc atccccagcc tggcctgcca gcctgatgga
    43141 taacagtggc tttcgtcgcg cactggctcc cagggcttga ggaaatggcg attctctgcg
    43201 taataatccg ccagacgcca ggcatcacga tcatgcacca gacgcacgac cagtcggtct
    43261 gtggttaagc gcacttttgg cacgttactg cgatagccaa acattctata cctactcctt
    43321 cccgtaacgt ctttaatacg ctgtaagtat tactatacct gcgcaaccag accctgtgaa
    43381 aagtccaaaa tgccattttc tgcattattc acatatttga taaaaactct ctatcaaagc
    43441 gacgttttga taaaaaaata ttgtccccga agtgatggga aaagtcttag cggcaatgca
    43501 gaatagaaag tgctcatctt ttatttcccg ggaggggaaa tgtcccgcgt gtcgcaggcg
    43561 aggaacctgg gtaaatattt cctgctcatc gataatatgc tggtcgtgct ggggttcttt
    43621 gttgtcttcc cgctgatctc tatccgcttc gttgatcaaa tgggctgggc cgccgtcatg
    43681 gtcggtattg ccctcggcct acgccagttt attcagcaag gtctgggtat tttcggcggc
    43741 gcaattgccg accgctttgg tgccaaaccg atgattgtta ccggtatgct gatgcgtgcc
    43801 gccggattcg ccacaatggg tatcgcccac gaaccgtggc tattgtggtt ttcatgcctg
    43861 ctctcgggac tcggtggcac gttgtttgat ccgccgcgtt cggcgctggt ggtgaaacta
    43921 atccgtccac aacagcgtgg tcgttttttc tcgctgttga tgatgcagga cagtgccggt
    43981 gcggtcattg gcgcattgtt ggggagctgg ctgttgcaat acgactttcg cctggtctgc
    44041 gccacagggg ccgttttgtt tgtgctgtgt gcagcgttca atgcgtggtt gttaccggca
    44101 tggaaactct ccaccgtacg cacgcccgtt cgcgaaggca tgacccgcgt gatgcgcgac
    44161 aagcgttttg tcacctatgt cctgacgctg gcgggttact acatgctggc tgtacaagtg
    44221 atgctgatgc tgccaattat ggtcaacgat gtggctggcg cgccctctgc cgttaaatgg
    44281 atgtatgcca ttgaagcgtg tctgtcgtta acgttgctct accctatcgc ccgctggagt
    44341 gaaaagcatt ttcgtctgga acaccggttg atggctgggc tgttgataat gtcattaagc
    44401 atgatgccgg tgggcatggt cagcggcctg caacaacttt tcaccctgat ttgtctgttt
    44461 tatatcgggt cgatcattgc cgagcctgcg cgtgaaacct taagtgcttc gctggcggac
    44521 gcaagagctc gcggcagcta tatggggttt agccgtctgg gtctggcgat tggcgacgct
    44581 attggttata tcggtggcgg ctggctgttt gacctgggca aatcggtgca ccagccagag
    44641 cttccgtgga tgatgctggg cattattggc atcttcactt tccttgcgct gggttggcag
    44701 tttagccaga aacgcgccgc gcgtcgtttg cttgaacgcg acgcctgatt tgatactccc
    44761 ccctgactcc tccatactga aagtgaactg acagccaaat ggaggagtca cgtgaagcta
    44821 tacatttacg atcactgccc ttactgcctc aaagcccgca tgattttcgg cctgaaaaat
    44881 atccccgtcg aattacatgt tctgctcaac gacgacgcag aaacacccac ccggatggtc
    44941 ggtcaaaaac aggttcccat tctgcaaaaa gatgacagcc gctatatgcc agaaagcatg
    45001 gatatcgttc actatgtcga taaactcgac ggcaaaccgt tactgaccgg caaacgttcc
    45061 cctgccattg aagagtggct gcgcaaggtc aatggctacg ccaacaaact gctgttgccg
    45121 cgttttgcca aatcggcatt tgatgagttt tctactcccg ccgcgcgcaa atatttcgtc
    45181 gacaagaaag aggccagcgc gggtaatttt gccgacctgc tggcccactc tgacggtctg
    45241 attaagaata tcagcgatga tttacgtgcg ctggacaaac tgatcgtcaa accgaacgcc
    45301 gtgaatggcg aactttcgga agatgatatt cagctattcc cgctactgcg taatctgacg
    45361 ctggtagccg gaattaactg gccaagccgc gttgctgatt accgcgataa tatggcgaaa
    45421 cagacacaaa tcaatttgtt atcatcaatg gcgatttaat cctgactcgc cgcggcagcc
    45481 tgtattcgcg ggttgccgcc gttttcagtt ttttctgctg gcgagtttat cgctggtgac
    45541 acatgctgcg ttgtgtcagg atattgacgc agatttcatg tcgaggacta ccatgaacaa
    45601 gtttttattt gccgctgcat tgatcgtcag tggcctgctc gttggctgta atcaactcac
    45661 ccaatacacc atcaccgaac aagaaattaa ccagtcgctt gcgaaacata ataatttctc
    45721 aaaagatatt ggtttacccg gtgtggctga cgcccatatt gttctgacaa acctgaccag
    45781 ccaaattggt cgcgaagagc cgaataaggt tgccctaacc ggagacgcca atctggacat
    45841 gaactccctg ttcggtagcc agaaagcgac catgaaactg aagctgaaag cgctgcccgt
    45901 gtttgataaa gaaaaaggcg cgatcttcct gaaagagatg gaggtggtcg atgcgacggt
    45961 acaaccggaa aaaatgcaaa cggtgatgca aacgttgctt ccctatctga accaggcatt
    46021 acgcaattac tttaaccagc aacctgctta cgtcctgcgc gaagatggca gccagggcga
    46081 agcaatggcg aaaaaactgg cgaaaggcat tgaagtgaag ccaggcgaaa ttgtcattcc
    46141 atttactgat taatcacgag ggcgcattcg cgccctttat ttttcgtgca aaggaaaacg
    46201 tttccgctta tcctttgtgt ccggcaaaaa catcccttca gccggagcat agagattaat
    46261 gactgcacca tcccaggtat taaagatccg ccgcccagac gactggcacc ttcacctccg
    46321 cgatggcgac atgttaaaaa ctgtcgtgcc gtataccagc gaaatttatg gacgggctat
    46381 cgtaatgccc aatctggctc cgcccgtgac caccgttgag gctgccgtgg cgtatcgcca
    46441 acgtattctt cacgccgtac ctgccgggca cgatttcacc ccattgatga cctgttattt
    46501 aacagattcg ctggatccta atgagctgga gcgcggattt aacgaaggcg tgttcaccgc
    46561 tgcaaaactt tacccggcaa acgcaaccac taactccagc cacggcgtga cgtcagttga
    46621 cgcaatcatg ccggtattag agcgcatgga aaaaatcggt atgccgctac tggtgcatgg
    46681 tgaagtgaca catgcagata tcgacatttt tgatcgtgaa gcgcgcttta tagaaagcgt
    46741 gatggaacct ctgcgccagc gcctgaccgc gctgaaagtc gtttttgagc acatcaccac
    46801 caaagacgca gccgactatg tccgtgacgg aaatgaacgg ctggctgcta ccatcactcc
    46861 gcaacatctg atgtttaacc gcaaccatat gctggttggt ggcgtgcgtc cgcacctgta
    46921 ttgtctaccc atcctcaaac gcaatattca ccaacaggca ttgcgtgaac tagtcgccag
    46981 cggttttaat cgagtattcc tcggtacgga ttctgcgcca catgcacgtc atcgcaaaga
    47041 gagcagctgc ggctgcgcgg gctgcttcaa cgccccaacc gcgctgggaa gttacgctac
    47101 cgtctttgaa gagatgaatg ctctgcagca ctttgaagca ttctgttctg taaacggccc
    47161 gcagttctat gggttaccgg tcaacgacac attcatcgaa ctggtacgtg aagagcatca
    47221 ggttgctgaa agcatcgcac tgactgatga cacgctggtg ccattcctcg ccggggaaac
    47281 ggtacgctgg tctgttaaac aataaaaatc gtagcttcct gttgtcatta ggttatttta
    47341 cctgtataaa taaccagtat attcaacagg gggctattat gcgaattgaa gtcaccatag
    47401 cgaaaacttc tccattgcca gctggggcta ttgacgccct ggctggcgaa ctttcccgcc
    47461 gtattcagta tgcgtttcct gataatgaag gccacgtatc agtacgttat gccgcagcga
    47521 ataatttatc ggttattggc gcgacaaaag aagataaaca gcgcattagc gaaattctcc
    47581 aggaaacgtg ggaaagcgcc gatgactggt ttgtcagcga ataatatgca gtgatttttg
    47641 tttttgccgg gtcgccccgg cttttttatt ttacttgcga att
//
LOCUS       DAZFAB010000025        45153 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZFAB010000025 DAZFAB010000000
VERSION     DAZFAB010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.025"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..839
                     /gene="espX2"
                     /locus_tag="ACHJWA_001686"
     CDS             <1..839
                     /gene="espX2"
                     /locus_tag="ACHJWA_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspX2"
                     /protein_id="HFO4308311.1"
                     /translation="LNFNEQQGLLDKDNKCYILLSSDNSGRVMRLSHQALISMLEPEV
                     KKKTIWNNYSIYPSLQDTHEVVRDDPETICMRAFPLFAKGWEYAQKNKKHQLILNALG
                     FKGYIRDIFMSAIMRKTDFVLECNNQPTELNSSFSSLMNDSDQWQQHTLKDKHYANLL
                     TMLDLNDASESDKSKIFFCLSAVFANISHSNVFNGIPDASKTLKRYAFALLAKAHSLD
                     ESMISNQTFNTYKTVLLDFNNLSNEEANQLRISSLYRDMVRYAQYRFSKVLSEWTPDA
                     WL"
     gene            1123..3273
                     /gene="dinG"
                     /locus_tag="ACHJWA_001687"
     CDS             1123..3273
                     /gene="dinG"
                     /locus_tag="ACHJWA_001687"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFO4308312.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDMLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            3301..4263
                     /gene="ybiB"
                     /locus_tag="ACHJWA_001688"
     CDS             3301..4263
                     /gene="ybiB"
                     /locus_tag="ACHJWA_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFO4308313.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREEMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WIQRCLAGREPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            4404..5489
                     /gene="hcxB"
                     /locus_tag="ACHJWA_001689"
     CDS             4404..5489
                     /gene="hcxB"
                     /locus_tag="ACHJWA_001689"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFO4308314.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDDFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            5556..5615
                     /gene="ybiE"
                     /locus_tag="ACHJWA_001690"
     CDS             5556..5615
                     /gene="ybiE"
                     /locus_tag="ACHJWA_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFO4308315.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(5718..5978)
                     /gene="ybiJ"
                     /locus_tag="ACHJWA_001691"
     CDS             complement(5718..5978)
                     /gene="ybiJ"
                     /locus_tag="ACHJWA_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFO4308316.1"
                     /translation="MKTINTVVAAMALSALSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(6243..6509)
                     /gene="ybiI"
                     /locus_tag="ACHJWA_001692"
     CDS             complement(6243..6509)
                     /gene="ybiI"
                     /locus_tag="ACHJWA_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFO4308317.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKESQLR"
     gene            complement(6583..7269)
                     /gene="ybiX"
                     /locus_tag="ACHJWA_001693"
     CDS             complement(6583..7269)
                     /gene="ybiX"
                     /locus_tag="ACHJWA_001693"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFO4308318.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFIWIQSMIRDDKKRAMLFELDKNIQNIQSLKSRYGENEEILSLLNLY
                     HNLLREWSEI"
     gene            complement(7311..9593)
                     /gene="fiu"
                     /locus_tag="ACHJWA_001694"
     CDS             complement(7311..9593)
                     /gene="fiu"
                     /locus_tag="ACHJWA_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HFO4308319.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSIAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTATLNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTASIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            complement(9858..10118)
                     /gene="mcbA"
                     /locus_tag="ACHJWA_001695"
     CDS             complement(9858..10118)
                     /gene="mcbA"
                     /locus_tag="ACHJWA_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFO4308320.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            10394..11320
                     /gene="rlmF"
                     /locus_tag="ACHJWA_001696"
     CDS             10394..11320
                     /gene="rlmF"
                     /locus_tag="ACHJWA_001696"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFO4308321.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELKQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVTFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            complement(11317..13542)
                     /gene="ybiO"
                     /locus_tag="ACHJWA_001697"
     CDS             complement(11317..13542)
                     /gene="ybiO"
                     /locus_tag="ACHJWA_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFO4308322.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEF
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQILSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLGSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGATPAEPLPPGEPT
                     L"
     gene            complement(13659..14381)
                     /gene="glnQ"
                     /locus_tag="ACHJWA_001698"
     CDS             complement(13659..14381)
                     /gene="glnQ"
                     /locus_tag="ACHJWA_001698"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFO4308323.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            complement(14378..15037)
                     /gene="glnP"
                     /locus_tag="ACHJWA_001699"
     CDS             complement(14378..15037)
                     /gene="glnP"
                     /locus_tag="ACHJWA_001699"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFO4308324.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            complement(15175..15921)
                     /gene="glnH"
                     /locus_tag="ACHJWA_001700"
     CDS             complement(15175..15921)
                     /gene="glnH"
                     /locus_tag="ACHJWA_001700"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFO4308325.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            complement(16325..16828)
                     /gene="dps"
                     /locus_tag="ACHJWA_001701"
                     /gene_synonym="pexB"
     CDS             complement(16325..16828)
                     /gene="dps"
                     /locus_tag="ACHJWA_001701"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFO4308326.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            complement(17127..18014)
                     /gene="rhtA"
                     /locus_tag="ACHJWA_001702"
     CDS             complement(17127..18014)
                     /gene="rhtA"
                     /locus_tag="ACHJWA_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFO4308327.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            18249..18314
                     /locus_tag="ACHJWA_001703"
     CDS             18249..18314
                     /locus_tag="ACHJWA_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4308328.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            18367..18882
                     /gene="ompX"
                     /locus_tag="ACHJWA_001704"
     CDS             18367..18882
                     /gene="ompX"
                     /locus_tag="ACHJWA_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFO4308329.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(18931..20514)
                     /gene="opgE"
                     /locus_tag="ACHJWA_001705"
     CDS             complement(18931..20514)
                     /gene="opgE"
                     /locus_tag="ACHJWA_001705"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO4308330.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTKGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(20786..20914)
                     /gene="mntS"
                     /locus_tag="ACHJWA_001706"
     CDS             complement(20786..20914)
                     /gene="mntS"
                     /locus_tag="ACHJWA_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFO4308331.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            21100..21567
                     /gene="mntR"
                     /locus_tag="ACHJWA_001707"
     CDS             21100..21567
                     /gene="mntR"
                     /locus_tag="ACHJWA_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFO4308332.1"
                     /translation="MSRRAGTPTAKKVTQLVNEEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            21564..22682
                     /gene="ybiR"
                     /locus_tag="ACHJWA_001708"
     CDS             21564..22682
                     /gene="ybiR"
                     /locus_tag="ACHJWA_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFO4308333.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVTL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(22740..23660)
                     /gene="ldtB"
                     /locus_tag="ACHJWA_001709"
     CDS             complement(22740..23660)
                     /gene="ldtB"
                     /locus_tag="ACHJWA_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO4308334.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            23879..25471
                     /gene="ybiT"
                     /locus_tag="ACHJWA_001710"
     CDS             23879..25471
                     /gene="ybiT"
                     /locus_tag="ACHJWA_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFO4308335.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            complement(25639..26904)
                     /gene="ybiU"
                     /locus_tag="ACHJWA_001711"
     CDS             complement(25639..26904)
                     /gene="ybiU"
                     /locus_tag="ACHJWA_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFO4308336.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPSQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(27056..27871)
                     /gene="ybiV"
                     /locus_tag="ACHJWA_001712"
     CDS             complement(27056..27871)
                     /gene="ybiV"
                     /locus_tag="ACHJWA_001712"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFO4308337.1"
                     /translation="MSVKVIVTDMDGTFLNDVKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            complement(28017..30448)
                     /gene="ybiW"
                     /locus_tag="ACHJWA_001713"
                     /pseudo
     CDS             complement(28017..30448)
                     /gene="ybiW"
                     /locus_tag="ACHJWA_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
     gene            complement(30454..31353)
                     /gene="ybiY"
                     /locus_tag="ACHJWA_001714"
     CDS             complement(30454..31353)
                     /gene="ybiY"
                     /locus_tag="ACHJWA_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFO4308338.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDPRLCLEGCELCAKTAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQSLTVCGE
                     VKSVEEIMVTVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            31484..32146
                     /gene="fsa"
                     /locus_tag="ACHJWA_001715"
     CDS             31484..32146
                     /gene="fsa"
                     /locus_tag="ACHJWA_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO4308339.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLEVVLP
                     ELHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTTEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPRA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWQGAFGRT
                     SI"
     gene            complement(32325..33074)
                     /gene="moeB"
                     /locus_tag="ACHJWA_001716"
     CDS             complement(32325..33074)
                     /gene="moeB"
                     /locus_tag="ACHJWA_001716"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFO4308340.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDSVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHNLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFSENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(33074..34309)
                     /gene="moeA"
                     /locus_tag="ACHJWA_001717"
     CDS             complement(33074..34309)
                     /gene="moeA"
                     /locus_tag="ACHJWA_001717"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFO4308341.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADITSGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLHRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VEVGEWVEVEPFNALFGGL"
     gene            34513..35478
                     /gene="iaaA"
                     /locus_tag="ACHJWA_001718"
                     /pseudo
     CDS             34513..35478
                     /gene="iaaA"
                     /locus_tag="ACHJWA_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
     gene            35465..37336
                     /gene="gsiA"
                     /locus_tag="ACHJWA_001719"
     CDS             35465..37336
                     /gene="gsiA"
                     /locus_tag="ACHJWA_001719"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFO4308342.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKEAVARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            37356..38894
                     /gene="gsiB"
                     /locus_tag="ACHJWA_001720"
     CDS             37356..38894
                     /gene="gsiB"
                     /locus_tag="ACHJWA_001720"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFO4308343.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            38912..39832
                     /gene="gsiC"
                     /locus_tag="ACHJWA_001721"
     CDS             38912..39832
                     /gene="gsiC"
                     /locus_tag="ACHJWA_001721"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFO4308344.1"
                     /translation="MLNYVIKRLLGLIPTLFIVLVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIARRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            39835..40746
                     /gene="gsiD"
                     /locus_tag="ACHJWA_001722"
     CDS             39835..40746
                     /gene="gsiD"
                     /locus_tag="ACHJWA_001722"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFO4308345.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            40924..43272
                     /gene="pdeI"
                     /locus_tag="ACHJWA_001723"
     CDS             40924..43272
                     /gene="pdeI"
                     /locus_tag="ACHJWA_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO4308346.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGQVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAKNQLAREKLIIYNSEGDYNIIDSVDTEYIEKTSAVPNNALFEIYFYYPG
                     GNLLNASDKLFYLPFAFIIIVLLVVYLMTTRVFRRQFSEMTELINTLAFLPDSTDQIE
                     ALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQ
                     YLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCR
                     ENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDH
                     MFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILGAEALCR
                     WVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINV
                     SPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGLRI
                     SIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAE
                     GVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            43280..44608
                     /gene="dgcI"
                     /locus_tag="ACHJWA_001724"
     CDS             43280..44608
                     /gene="dgcI"
                     /locus_tag="ACHJWA_001724"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO4308347.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(44678..45007)
                     /locus_tag="ACHJWA_001725"
     CDS             complement(44678..45007)
                     /locus_tag="ACHJWA_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4308348.1"
                     /translation="MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAK
                     VKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSKWERNEIKPSGPALRILNTLAIK
                     GPEVFAK"
     gene            complement(44997..>45153)
                     /locus_tag="ACHJWA_001726"
     CDS             complement(44997..>45153)
                     /locus_tag="ACHJWA_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO4308349.1"
                     /translation="KSGLSSKGSKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFL
                     IEVKNER"
BASE COUNT        10931 a        10982 c        11995 g        11245 t
ORIGIN      
        1 aattgaattt caatgagcag caaggattgt tagataaaga taataaatgt tatatcctgt
       61 tatcatccga taactctggg cgagtaatgc gcttatcgca ccaggcgtta atctcaatgc
      121 ttgagccaga ggttaagaaa aagactatct ggaataatta ctctatttat ccatctttgc
      181 aggataccca tgaagttgtc agagatgatc cagaaacgat ttgtatgcgt gcatttcctt
      241 tatttgcaaa aggatgggaa tatgcgcaga aaaataagaa acatcaattg atcctcaatg
      301 ctcttggatt caaaggttat attcgtgata tatttatgtc agcaataatg cgaaaaacag
      361 attttgtgct cgaatgcaat aatcaaccga cagaacttaa ttcatcattt tcctctttga
      421 tgaacgattc agatcaatgg caacagcata ctctgaaaga taaacattac gctaatctat
      481 taacgatgct ggatctcaat gatgccagtg aaagtgataa gtctaaaata tttttctgcc
      541 tgtctgccgt atttgcaaat atttctcaca gtaatgtctt taacggtatt cctgatgcgt
      601 caaaaacttt aaaacgttat gcatttgctt tattggcgaa agctcattct ttagatgaaa
      661 gtatgataag caatcaaact tttaatacat acaaaacggt attgcttgat ttcaataatc
      721 tgtctaatga agaggccaac cagttgcgca taagttcgtt atatcgtgat atggtccgct
      781 atgcacaata tcgtttttct aaagttttat ccgagtggac gccagacgca tggctgtaaa
      841 gatcgcagca gaataatggc attgatggcc ctggctcagg ccagggtcgg caataccggt
      901 tcctgcgaag cgatgacggc ggcaattgta accacgaaat gcggtatcac ttgctgtttt
      961 ctgccctgaa gactggcgct cactgtgccg tcgaattttc aatcaaatat attgcgagag
     1021 ggcgtgtttg cgagccgctt tccagaaaca gaaaaaccat tacccctgaa aaccgaaaaa
     1081 tgccacaata ttggctgttt atacagtatt tcaggttttc tcatggcatt aaccgccgcg
     1141 cttaaagcgc aaattgccgc ctggtataag gcgcttcagg aacagatccc cgactttatt
     1201 ccccgtgcgc cgcagcggca gatgattgcg gacgtcgcca aaacgctggc cggagaagaa
     1261 gggcggcatc tggcgattga agcccccact ggcgttggga aaacgctctc ctatttgatt
     1321 cccggcatcg ccattgcccg cgaagagcaa aaaacgctgg tggtgagtac cgccaacgtg
     1381 gcattgcagg atcagatcta cagcaaagat ttgccactgc tgaaaaagat cattcccgat
     1441 cttaaattca ctgccgcttt tgggcgtggg cgctacgttt gcccgcgtaa tctgacggcg
     1501 cttgccagca ctgagcccac gcaacaggat ctgctggcgt ttcttgacga cgaactgacg
     1561 ccaaataatc aggaagagca aaaacgttgt gcgaagctga agggcgatct cgacacttat
     1621 aaatgggatg gtctgcgcga tcatacggat atcgctatag atgacgatct ctggcgtcgt
     1681 ttaagtaccg acaaagccag ctgcctcaac cgcaactgtt actactatcg cgaatgcccg
     1741 ttttttgtcg ctcgtcggga aattcaggaa gcggaagtgg tggtggcaaa ccatgcgctg
     1801 gtgatggcgg cgatggaaag cgaagccgta ttgcctgacc cgaaaaattt actgctggtg
     1861 ctggacgaag gccatcacct gccagatgtg gcgcgggatg cgctggagat gagtgccgaa
     1921 atcaccgcgc catggtatcg gctacagctg gacttgttca cgaaactggt cgctacctgc
     1981 atggagcagt ttcgcccgaa gaccatcccg ccgttggcga tccctgaacg tttgaatgcg
     2041 cattgtgaag agctgtatga gcttatcgcc tcattaaaca acattctcaa tctctacatg
     2101 cctgccgggc aggaagcaga acaccgtttt gcgatgggcg aactgcctga tgaagtgctg
     2161 gagatctgcc agcggctggc aaaactcacc gagatgctgc gtggtctggc ggagttattt
     2221 cttaacgatt taagtgagaa aaccggcagc catgacattg tacgtctgca tcggttgatt
     2281 ttgcagatga accgcgcgtt ggggatgttc gaggcgcaaa gcaaactctg gcggctggct
     2341 tcgctggcgc aatcttccgg tgcaccggtg accaaatggg cgacgcggga agagcgcgaa
     2401 gggcagctac acctctggtt tcactgcgtg ggaatacgtg tcagcgatca gctggaaagg
     2461 ctgctgtggc gcagtattcc gcacattatt gtcacctccg caaccttgcg ttcgctgaac
     2521 agtttttcgc gtttgcagga gatgagcggg ctgaaagaga aagcgggcga ccgttttgtg
     2581 gcgctggatt ccccctttaa ccactgcgaa cagggcaaaa ttgttattcc ccggatgcgc
     2641 gttgagcctt ccatcgacaa cgaagaacag catattgctg aaatggcggc ctttttccgt
     2701 gagcaggtgg agagcaaaaa acatctcggt atgttggtgc tgtttgccag cgggcgtgcg
     2761 atgcagcgct ttctcgacta tgtgacggat ttacgtctga tgttgctggt gcagggcgat
     2821 cagccgcgtt accgtttagt tgaactgcac cgcaaacgcg tcgccaacgg tgagcgtagt
     2881 gtgctggtgg gcttacagtc atttgccgaa gggcttgatt tgaaaggtga tatgctcagc
     2941 caggtgcata tccacaaaat cgcttttccg cctatcgaca gcccggtggt gatcactgaa
     3001 ggggaatggc tgaaaagcct caaccgctat ccatttgagg tgcaaagcct gccgagcgcc
     3061 tcgtttaacc tgattcagca ggttgggcga ctgattcgaa gtcacggttg ctggggcgaa
     3121 gtggtgattt acgataaacg cttgctgacc aaaaattatg gcaagcgact actggatgca
     3181 ttaccggtat ttccgataga gcaaccggaa gtccctgaag gtatagttaa aaagaaagaa
     3241 aaaacgaaat ccccacgccg tcggcggcgt taatgatgtg agtcaggtaa ggagtcgtaa
     3301 atggactatc gcaaaatcat taaagagatc gggcgcggga aaaaccacgc gcgcgattta
     3361 gaccgggata ctgcgcgcgg tctgtatgcc catatgctca atggtgaagt ccctgacctt
     3421 gagttgggcg gtgtattgat tgcgctgcgt atcaaagggg aaggggaagc agagatgctc
     3481 ggcttttacg aagccatgca aaatcacacc atcaagctga cgccgcctgc aggcaagccg
     3541 atgccgattg tcatccccag ttacaacggc gcgcgtaaac aggccaacct gacgccgttg
     3601 ttggcgattc tcctgcacaa actcggtttt cctgtggtgg ttcacggggt tagcgaagat
     3661 ccaactcgcg tgctgaccga aaccattttc gaactgatgg gtattacgcc aacgttgcac
     3721 ggcgggcagg cgcaggcgaa gctcgacgaa catcaaccgg tgtttatgcc tgtcggcgcg
     3781 ttttgcccgc cgctggaaaa acaactggcg atgcgctggc ggatgggcgt gcgtaacagt
     3841 gcacataccc tggcgaaact ggcgacgcca tttgccgaag gtgaggcact gcgtctttcc
     3901 agcgtttcgc atccggaata catcgggcga gtcgcgaagt tctttagcga tatcggcggg
     3961 cgggcgttac taatgcatgg tactgaaggc gaagtgtatg ctaatccgca gcgctgccca
     4021 cagatcaatc tcattgatcg tgaagagatg cgggtgctgt atgaaaaaca ggaccctgct
     4081 ggaagcgagt tactgccaca agcaaaagat ccggaaacca cggcgcagtg gattcagcgt
     4141 tgccttgctg gccgcgaacc gattcccgaa tcgctgaaaa tccagatggc ttgctgcctg
     4201 gtggctacgg gtgaagcggc aactatcagc gatggcctgg cgcgcgttaa tcaggcattt
     4261 taattctttt ctcagccgga tgagccatgt catccggctt ttaccccacc gcaatatgaa
     4321 attcctgcat ctttattgac cttcccgcgc ccggcgtgca gcataaaaaa acaacaaaca
     4381 cataacataa acaggagtta accatggaaa gtggtcatcg ctttgatgct cagacgctgc
     4441 acagctttat tcaggctgta tttcgtcaga tgggtagcga ggaacaagaa gcgaaattag
     4501 ttgccgatca tttaatcgcg gcaaacctgg cagggcatga ttcacatggt attggcatga
     4561 tcccaagcta tgtacgctcc tggagtcagg ggcacctgca aattaaccat catgccaaaa
     4621 tcgttaaaga ggcgggggcg gcggtcacgc tcgatggcga tcgcgcattt ggtcaggtcg
     4681 cggcacatga agcgatggcg ctggggattg agaaagcgca tcagcacggt attgctgccg
     4741 tggcgctaca taactcgcat catatcggcc gtatcggtta ctgggcggag cagtgtgcag
     4801 cggcggggtt tgtctctatc cactttgtta gcgtggtcgg tattccaatg gtcgcaccgt
     4861 tccacggtcg cgacagccgc tttggcacga atccgttctg tgtggttttc ccacgtaaag
     4921 atgattttcc gctgttgctt gattacgcca ccagcgccat tgcatttggc aaaacccgcg
     4981 tcgcctggca taaaggcgtc cccgtgccgc caggttgcct gattgacgtt aacggcgtgc
     5041 cgacgaccaa tccggcggta atgcaggagt cgccgttggg ttcgctgttg acctttgccg
     5101 aacataaagg ctacgccctg gcggcaatgt gtgaaattct tggcggggcg ctttctggcg
     5161 gtaaaacgac gcatcaggaa acgttacaaa ccagtcccga tgccattctt aactgcatga
     5221 ccactatcat catcaacccg gaactgttcg gcgcgccgga ttgtagcgcg cagaccgaag
     5281 cctttgccga gtgggtgaaa gcctcgccgc atgatgacga taagccgatt ttgctcccgg
     5341 gcgagtggga agtgaacacg cgtcgcgaac ggcaggagca ggggattcca ttggatgcgg
     5401 gaagctggca ggccatttgt gatgcggcgc ggcagattgg tatgccggaa gagacgttgc
     5461 aggctttctg tcagcagtta gccagctaaa aaaaagcccg tccagtggcg gacgggcaaa
     5521 caagggtaac ataggatcaa tgagggttag agcatatgcg tctgtcggca aacagacagg
     5581 gaaatacttg tgcaggacgt agcgtaaacg cctgatccgt ccaacggttc ggattttgta
     5641 ggcctgataa gacgcggtag cgtcgcatca ggcatctgtt gccggatgcg gcgtaaacgc
     5701 cttatccgtc ctacgaatta cttataaatt accgcagtac cgcttaattt attgttgttg
     5761 gtggcggaag tgatgctata tccactggca ccggctgccg ccgctttctc agccagtttc
     5821 gcttccaggg catcgagggt ggatgcgcca tcggcagaaa ccacgccgat tttattcatg
     5881 ttctgtgcct gggatgccgt taccggttcc gcggcaaaca cgccaaatga cagagctgaa
     5941 agagccatag cagcaacaac agtattgata gttttcataa ttaatctctc gcaggtgatt
     6001 tttgtataag tgacgttgtt tcgtcgatgt gatgagtatc acgttttttt acgagagata
     6061 aaatcgaaga gaattgaccg ccttgttcaa ataaattgat tgatatctaa catactgata
     6121 aaccatttat ttgttgtact tatggacttg ttttatgcag cgtggcagtc acgacagcgt
     6181 taaaggcacg gaatgccacg caaaatacaa attatactcg ctatacatct cgccagttgt
     6241 agttaacgta actggctttc tttcgaacct ctgcgattat atcctgtata agctggtttt
     6301 tgtaaatctt tctcctgctg acaatgaatg cataagcgca cgccaggaat ggcttcccga
     6361 cgggcttgcg ggatgggggc accgcactct tcacattcat acaggctttc gccacgcgga
     6421 atttcacccc gagcgcgggc aatcgcatct tcaattgtac tgttgatctg ttcgttgacg
     6481 gcgtcatcgt tagcccaacc ggatgccata tcgtcctccc catatcaatg cttgtatagt
     6541 taagtgtagc taatataggg acgaacctgg gcagttcaag cgtcagatct ccgaccattc
     6601 ccgcagcaga ttatgataaa gattgagcag cgacaggatc tcttcatttt caccatagcg
     6661 gcttttcagc gactgaatat tctgaatatt tttgtccagt tcaaacagca tggcgcgctt
     6721 tttatcatcg cggatcatcg actggatcca tataaatgat gccactcgta cgccgcgggt
     6781 tacgggtgtc acgcaatgca ggctgctgga gggatacaac accagatcac ctgccgggag
     6841 ttttacccga tgttgtccga aggtgtcatt aacgaccagt tcgccgccgt cgtagctttg
     6901 tggatcgctt aaaaacagcg tggcagaaag gtcagtgcgc atccagccgt tttgtggatg
     6961 gctgcgtact gcgccatcca catgaaaacc ataggtttca ttgttctgat agcgattaaa
     7021 cagcggcgtg gaaagggtac gcggcaaggc cgcggcaaag aacaaagcat gttggttaac
     7081 cgcgttcagc acctcatttt gcagggcggc gtataacgtg ctgcgggtgt ctacctgttg
     7141 attgttctta acttgcgcac cttgtgcgcc ggtggtgacg cgtccatcca cccattcggc
     7201 ttgttccagt tgttcgcgaa aacgggcgac gtcctgtggc gataacacgc cgggaatgtg
     7261 gtacatcata caagttctcc aaaaagtggg gcctgcgccc cacatctgaa tcagaaatgc
     7321 atattggctg tgagcaagaa ggttcttggc tcgcccgggt gataacggta gccgctctta
     7381 ttgattgagg cgacgtaatc ggtatcaaac aggttgtaga cgtttagctg gaagtcgaga
     7441 ttgcgattaa ccctataccc cagtttggca tcggcgaccc agtaaccttc ggtaaacgct
     7501 ggcgttccca ccgcgccgtc tgaaccttta tgcatactgc cgatatagcg tgcgcccgcg
     7561 ccaacagaga tatcatcggt tgcctgatat tggctccata aggtgaaggc gtgctccggg
     7621 gtatacggca gcgatgagga accatcctgg gcaacatctt tgccgttttt gatggttgct
     7681 ttttgctggg tatagccgcc aatcacctgc cacgcgggag tgatattccc tgccacggat
     7741 atctcatagc cttcgacgcg tttcttaccg tattgcgagt aagtcccgtc atcattttgc
     7801 tcaacttcat tttcgatatc agtacggaac agcgcggcgg tgagcaacag gcgtttatcc
     7861 agaacctgcc atttggtgcc aatctcgctg gtgttggctt tttgcggttt aaaatcggtg
     7921 cggttggcac tgttaccgct gccagactgc gcaagggcga agttgttgcc gcccggaggc
     7981 tgctgggaaa cggcatagtt aatatagaca ttgccgtttt ccgtcaggtg atacagcgcc
     8041 ccggctttcc agttcaccag attgcccgac ttggcggtgt cgacggtggt gaccggagaa
     8101 ccttttgcca caccagctgg gcaggtgatg gcaccgcgtc cgctgccgcc gcaggcggtg
     8161 gcactgtcat attcagtatg ataattatcc agacggatcc cgccgttcag ctcaaaatca
     8221 cgggtgattt gcagcgtatc gaatgcgtaa attgcgaagg tatccgtctg accattggcg
     8281 tttgcgccgt tgcgcgtcag gccgccggga tgaatgctgc tgtcaggatg ataaatattt
     8341 accgcaggta acgtcaccgg attaacgccg tagttagtct gcgtttcacg ggtaaattcc
     8401 acgccggtac tgacatcatg accgatagaa gcggtataga acgtcgaggt caggttggtc
     8461 tggttggtga gaattttatt actcacatct ttggtattcg ccgtgcgtga ccacgtccag
     8521 ctattcacat cgctggtggg ctgggtaata ttcgacgccc cacccataat cgccgtcatc
     8581 aggtaatcct gcttcacgcg cgaccaacgg gtagtgttgc gaatggtggt gttatcgttg
     8641 atatcgtgct caaaacgcat ggtggcggtg tcggtggtcg aatcgtcgta atcggaatcc
     8701 gtgccgtaaa agttatgcgt atcaactttt ccggaatgat tcagggtcgc cgttcctgca
     8761 gatggggcag aatagcccgg caaaccgatg gttggaatgc cgccgtctgg cgtgttgtgc
     8821 tgggtgacat gcagataatt aagatacaaa cgattcgctg taccaaggcc aaaagcgata
     8881 gaaggggcga cgccgtaacg ctcatttttg actttgtcgc gtccggcatc gtgcgttttt
     8941 tcgcccatca cattcaggcg caccgcagtg gtatcaccaa tgacctgatt gacgtccagc
     9001 gtgccgcggc ggaaccaggc gctgccaata ctggcggagg cgtcaatgcc ggaatcattg
     9061 cgcggctgct tgctgatcat attgatcgag cccgtcggcg cgctgcgccc gtagtcggtg
     9121 ccggaaggcc ctttaatcac ttcgacctgc tcagtattga aggtgtcgcg cgagacgctg
     9181 ccgatatcgc gaatgccatc aatataaata ctgttagagg tatcagcacc gcgcatataa
     9241 atggcgtcgc cagtggtgga gttaccgttc tcacccgcaa aaaacgcacc cacgccgggg
     9301 acgtttttga gcgcatcggt aaggttggtt gcgccctgat ctttaatcac ttgttcagaa
     9361 atcaccgtca tcgtgcgggt agtatccgct accggacgcg agaatttcgg atcggcagat
     9421 tgttgtggcg cataaagcga aggcgttgat gcttcgacaa ccagcgtgtc atccgcgtta
     9481 gtttgccctt cggcggcgag tgcctgagcc acaggcgtga tgccaataca aagaccggca
     9541 aagaacgtga gcgaatgaaa ttgtctggca gggaaattgc gattgttttc cattttgcag
     9601 gtgacttttt cttatatgtt gaaattttca ctgccacgag aaagcctctc tgcggatgcg
     9661 atgccactgt taatctcgtg atagtgttcg tgcaagtgat aataattttg ataagcatta
     9721 tcacttggcg cgattttcta tcacccattg cgcgaaaaag aaatacattt atttacattg
     9781 cgttcatatt tgatgttttt tgattacaaa acaggcgaaa actgcatcca gaggcgaatg
     9841 cagttacgtt ggtggggtta tttgtagatg gttgcagtac cgaacatctg gtcatttccg
     9901 cctgcggaat taatgacata acctttcgcg ccttgctcgc gcgctttttc tgccagttta
     9961 tcctccagat cgcttaggtt tgatgcgcca gttgccgaaa cggtaccggc gggccgtagc
    10021 tgaccgctgt ccaggttact catcggttgc gcggcataag ccgtgagaga gattccgctc
    10081 aggacggtgg caatcagtag tgtgaggcac tttttcataa tgacatcctc ataaatacaa
    10141 cgggtagtgc ctgataagtg taggtcgcct gcgcgatgca aatgacgcaa aaaattgaca
    10201 gagatgtgct tattttttga acgatatatt tttacaaaat aagacaaatc aatgacatgc
    10261 ataatgctga tttttatgat gaattgccat aaaccatagc catgttaagg ttttggggaa
    10321 tgataaacgc agccagatat ttaccctgtc gcgttatgcg ggtatcttac gccgctgctt
    10381 aaaggagaat gctatgtccg cccagaaacc ggggttgcat ccgcgcaacc gtcatcacag
    10441 ccgctacgat ctcgccacgc tttgtcaggt caatcctgaa ctcaagcaat tcctcacgct
    10501 tacacccgcc ggggagcaaa gcgtagactt tgccaatccg ctggcggtga aggcgctcaa
    10561 taaggcgttg ctggcccatt tttacgccgt agcgaactgg gatatcccgg acggttttct
    10621 ctgcccgcca gtaccgggcc gggcggatta tattcatcac cttgccgatt tactggctga
    10681 agcaagcgga acaattccag ctaatgccag cattcttgat atcggcgttg gtgcgaactg
    10741 tatttatccg ctgattggcg tacatgaata tggctggcgt tttaccggta gtgaaaccag
    10801 cagccaggcg ttaagcagcg cgcaggcgat catcagtgct aatccggggc ttaatcgcgc
    10861 cattcgtctg cgtcggcaaa aagagagtgg ggcgattttt aacggcatca tccataaaaa
    10921 cgagcaatac gacgcgacct tgtgtaaccc gccattccac gattccgccg ctgcggcgcg
    10981 ggcaggtagt gagcgtaaac gccgtaacct ggggctgaac aaagacgatg cactgaactt
    11041 tggcggccag caacaggagt tgtggtgtga aggcggtgag gtcaccttta tcaaaaagat
    11101 gattgaagag agcaaaggct tcgcgaagca ggtgatgtgg tttacttcac tggtatctcg
    11161 tggtgaaaac ttaccgccgt tgtatcgtgc cctgacggac gtgggcgcgg tgaaggtggt
    11221 taaaaaagag atggcccagg ggcaaaagca gagtcgcttt attgcctgga cctttatgaa
    11281 cgacgagcag cgccgccgtt ttgtcaatcg ccagcgttaa agcgttggct cccccggcgg
    11341 taacggttca gccggggtcg cgcccggagc aggcagcacc tgataagtct gcaccggcgc
    11401 gcgaacgccc gccaggtcga aatgtttttt cacctggctg tcgagggcaa agcgtaccgt
    11461 ccactgtttg agtggcagcg tggtgaacga aacacgcagt gtaaacgcgg tattgcttaa
    11521 gccaacaatc ccggcaaagt tcggttcacc aataatcagc ccacgaattt cttcgttttc
    11581 cattaattcc gctaccgcat ctttcagtgc ctgattggct ttatcagcat cttcatggcg
    11641 atcaacatcg tagtttgcca ctaccgaacc aatgccgcgg acaaagttgg caaaggtggt
    11701 aatcgaagac cacggaatga tgtggtacgc cccggtatcc tggcgtacgc ccacggagcg
    11761 aatcgacatc cgttccacag tgccggtcaa cggcccaata gtcaccaaat ctccagtgtt
    11821 catgccgttt tcaaactgaa taaatacccc ggtgataata tctttcacca gcgtttgcga
    11881 accaaacgag atagccagac ctaatgcccc ggcacctgcc agcaatggcg cgatattgac
    11941 gccgatttcc gacaacacaa tcatgatggt gatggtactg ataatcaccg ccagcgcgtt
    12001 acgaaacagc gtaagcaggg tacgcgtgcg ggcgctgggt agcgggcggc catgaatatc
    12061 cgaagccagc cggttttcga tcaaactggc gagcaccgtc cagccaaccg ccgagaagaa
    12121 aagaatgagt gcgatacgga tcaggatatc tacggttttc tgccccgcgc cgttttgcag
    12181 ccagttccag aaatcgaaca atccccatgc gctcaacagc agcattaccg ccacgcagac
    12241 ggtcagaata cgcgccgttt tcagcgccgc cgaaagccag ccattcagcc gtttttgcaa
    12301 ctccggatag ttacgctgag tatgtggcga gagagtgatg gtttttgcca gccagcggga
    12361 aaacataccg gaaacaaatg ctgcgatacc aataatcgcc aggctgcgca ctgttgcacc
    12421 catcataaat ttcaggctgt tgcccggatc gaacaacgaa aagaaaaaca gcacgataaa
    12481 ataggcgctt gccagccagt gccacaccag cgcaaaggcg cggataaaca ggctgaaaaa
    12541 ggccagcgaa tgctccgcaa agttgagcaa atgctgggta atctcttttt tattacgaaa
    12601 gatcaggtac aacgcccaga cggtcatgca cagcataatg atgacgttcg ccagcgcacc
    12661 tatctgtaca ttcacctgat tagagataat cggcacggcc acaatcaggc cataacctat
    12721 caaactgcct aaccagctca ggcgacgact ccagtaacgg gcgctctcgt cctgaatcgt
    12781 gaacgggcgc agttccgcca cgtttgggca aaaaatcagg cgtagtacgg ctttgaaaaa
    12841 ttcaatcagg gcaaaggcgt tgagaaacaa actttgttgg aaagcgatgg tgcgactgcc
    12901 tgcattcagg ttgtcgctta atatctggcc gacaaataat gtcagtgcca gtaacagcag
    12961 gtcgataata aacgccccga taatcatcgc cggaagctgc agccagttac tgcgctcacg
    13021 atttttttgc cgcgcccact gacccatttt gcgatacagc ggcagtgcgc acaggcgaat
    13081 cagccagtaa aaaccaaaca ctaaaaccgc taacattgaa aaatgggtca gcgcattgct
    13141 gaaggtttgt ggattaaacg gcttatgcgg ggagccggtg atattgcgat aaagctgccc
    13201 gaagcgggcg gaaagggctt caccataatg gcggctgaat tcggtgacct tttgcagcac
    13261 ggtttgctct tcaaccagcg tcggcggcac gatcttcggg accggttcag caggtggcgt
    13321 cgcggcgacg gtgcgcaact ggtcgatcaa ctctttacgc gaggtgtcat tatccagcac
    13381 atccgccagt gcgccataag ccgctttttt ttgttcgata tccggttccg gggccggttc
    13441 agtcgttgag tccgttgtcg ttgtggttgt aacgccgggt atggataccg cgtgggcagg
    13501 tgcgcccagc aggcagaaga ggatgaacag gatccaccgc atgactcctc cagtgagaaa
    13561 acagagcaaa aagataagta tagatgctgg gggaggggtg attttaaatg agaggaatct
    13621 ggtgtacctc cctttcgggt gaaagggagg cagagaagtt aagagacgtg ctgtaaaaat
    13681 tcctgcaagc gctggctcgg cggattttta atcaacactt gtggatcgcc atcttccgca
    13741 atccggcctt tgtcgataaa gatcaggcgc gaagctactt tctcggcaaa accgatttcg
    13801 tgggtcacga tcaccatcgt catcccttct tcagccagat cttgcataac cttcagcact
    13861 tcatggcgca gttccgggtc aagagcggaa gtcggttcat caaacagcat cattttcggc
    13921 ttcaccgcca gcgcgcgggc aatcgccaca cgctgctgtt gaccaccaga aagttcggaa
    13981 gggtagtgat gtgcacgttc tgccagaccg actttcgcca gcagctcacg tgccagtttt
    14041 tccgcctctt ctttgttcgc gccacgcacg cgtagcgggc caaacatgac gttttccagc
    14101 gccgtcagat gcgggaagag gtaaaactgc tggaacacca tacctgcttc ctggcgaatc
    14161 aggcgctcgt caactttcgg atcgttaacc ttcaggccat cgacaatcag atcgccggag
    14221 gtgatttctt ccagtttgtt gatgcagcgc agcagggtcg atttaccgga accggacggc
    14281 ccgataatca ccacgacttc gccctgggca atgttcaaat cgatattgtg cagcacctgg
    14341 gttgggccaa agtgcttgga gacgttttta aattcaatca caggattttc atccttcttt
    14401 ccagacgacg cagaataaag ctcagcacca gggtaataat cagatagaac accgccacgg
    14461 cgctccagat ctcaagggcg cggaagttac cggcaataat ttcttgcccc tgacgggtca
    14521 gttccgccac gccgatcaca ataaacagcg aggtgtcttt aatgctgatg atccactggt
    14581 tacccagcgg cggcagcata cgacgcagtg ccagcggtaa aatgacgtag cgaatggttt
    14641 cccaacgtga aagaccgagc gccagtcctg cttcacgaaa acctttgtgg atagacagca
    14701 ccgcaccacg cgtgatttcc gcaatgtacg cgccggagtt gatcatgatg gtgaccaccg
    14761 ccgcagtaaa tgggtcgatg cgtaagtcat taaacgccat cggcagggca aaatagataa
    14821 acatcacctg gacgacgata ggtgtgccgc ggatcacttc aataaaaacc agcgcgacgt
    14881 ggttggctat ccaaccaccg aaggtgcgtg caaaacctgc cagcaatccg atcaccaggc
    14941 cgccagccag accgaggacc gatatccaca gggtcatttt ggcaccttca atcagaagcg
    15001 gaatggcagg ccagatagca ctccagtcaa actgcatatg ttgttcctgt taccgtggtg
    15061 taaataatca aattcagggt aattaatggc cccggagcgg gtttgcgccc tcaccctaat
    15121 cctctcccca gaggggagag ggaactgcca gtgcgcttta caggtgtagc gttattattt
    15181 cggttcagta ccgaaccatt ttttgtagat ttcgttgtaa gttccgttct cgcgcagggt
    15241 tttcaacgcg ccgttgactt tgtcacgcag ctcgtcgcta cctttcggga acgcaatacc
    15301 gtattgctgc gcttccagag agtcgcctac cgctttgaac tgaccgttac cggcggtttt
    15361 gatgaagtac agaatgtttg gcgtatcgtg cagaacggcg tctgcgcggt tggtgcccag
    15421 ttccatatag gcgttatcga tgttcgggaa ctgacgcaga tctttagttt tgatgtttgc
    15481 tttcgcgtaa tcaacggagc cagtgccgct cttcaccgca accactttcc cgtcgagatc
    15541 tttcacgctt ttcacatcgt tattgttagc tttcaccatc actaacaggc cgcttttgta
    15601 gtagccgtca gagaaatcga tcgctttttt acgctcgtcg gtgatggtaa tgcccgccag
    15661 cgccagatcg acgtttttgg tttgcagtgc cggaatgatc ccactgaaat ccatcggctt
    15721 cagttcgtaa tccagcttca gctctttagc gatggcagcc cacagatcaa cgtcaaagcc
    15781 cacatattta tcgccctgtt taaattcaaa cggaacgaag gcggtatccg tcgcgacaac
    15841 taatttttta tccgcggcat gagaagaaac cgcaaaagcc agggtcagtg cagccagtga
    15901 aacttttaat acagacttca tagcatttcc ttttttctat ccacgggacg atcccctgcg
    15961 tgacattcac atatatgaaa aaatcgtgcc agttttgcaa ctcattgatt tttcgagatg
    16021 cggcaaaaac gtgatgcacg atttataggg caacaccctg aagatgcacc attctggggc
    16081 accaaactgg tgcgctaaaa ttgtgcactc aacacagtga ttatttagcg taaaacctga
    16141 tgaaaaaaca atctttatgt aacgattgtg tgatgatgtg gatacaaaaa atttacttaa
    16201 tcagctgggg atagcagatg gatgcactaa ataagtgcgt tgaggcgggc tgcaaaacaa
    16261 aacggcctcc tgtcaggaag ccgcttttat cgggtactaa aattctgcac catcagcgat
    16321 ggatttattc gatgttagac tcgataaacc acaggaattt atccaggtcg cgagacgcgg
    16381 cggtcaggat atctgcggtg tcgtcatctt tcgcttcgcc aatcgctttg cgtacgtcat
    16441 tagcgacgat tgcgtaacgg tcagccagtt ctttcaggtg atcctgaacg ttgtggatgt
    16501 ccagcgggta acttttcagc ggggttttgc tgttgataac ttgagtggtc cccagagcta
    16561 caccgcccag ctgcactgca cgttctgcca tggtatccag atgatcgatc agtgcagtgc
    16621 ggaagccatc cagcatttca tgtacggcaa tgaagttagc gccgcgcatg ttccagtgcg
    16681 cttgtttggt aatcaaagaa agatcaataa actggataac ctggcgattc agcaactcta
    16741 ctgttgcttt tttctcgctg tcagagacat cgttgcgggt ataaagcaga ttggtcgctt
    16801 ttgatttaac taatttagcg gtactcataa tttcatatcc tcttgatgtt atgtcccagt
    16861 aattaacgag actaagtata gcaccggcta tgtgttccgc tattctggct gttcctatca
    16921 cactaatagt ggtaacaagc gtgaaaaaca aaactaataa agattcaatg agttagatat
    16981 attgataaga acaattctta tttatttgct cgccaggaat tttcggaata aatatgcttt
    17041 tgcataattc gatatataaa gacggtgtag aggaaaagta gcgagaaatt ctgcatggtt
    17101 atgcataacc atgcagaaat gtaaatttaa ttaatgtcta attcttttat tttgctctct
    17161 ttgcgtactg tcagcgtaga acccattgaa gcggcgataa ttgcgccgag cgccagcagc
    17221 tgtatgggtg tcagtgtttc tccgaggaaa atcatcccgg aaacggcagc cagcgccggt
    17281 tccatgctca tcagcgtacc aaatgtccgt gttggcaaac gggtgagggc aatcatttcc
    17341 agcgaataag gcagagcggt cgagagaata gcgaccgcca gacccaatgg aataaccgac
    17401 cagtgccaga gtgcttcacc agcctgaagc gctccaattg gcacgaaaat taacgctgca
    17461 atcaacgaac caattgccac cgtcgcaggg ccatgttccg ctcctgcgcg ttgcccactt
    17521 aaaatgtaaa tagcccaaca agccccggcc cccagtgcca gcgcacagcc ggttaaatcg
    17581 acatgggaaa cgtcttgccc cagcggtagc aggaaccaca gaccaagaac cgccagcaca
    17641 acccagacga aatctaccgg gcgacgagaa gagaacagcg ccaccgccag tggtccggtg
    17701 aactccagcg ccaccgcaat acccagcggt accgtctgaa tagagagata aaaaagataa
    17761 ttcatcccac ccagcgaaac gccgtaaaac aacagcggta accgttgctc tttggcaaag
    17821 cgcagtcgcc atggcttaaa gaacgcgatg aggatcagcg ttcctaatgc caggcgcagc
    17881 gcagtgacac ccggtgcgcc caccagagga aaaagtgact tagctaacga agctccacct
    17941 tgaatagacg ccatggcaac gagcaatatg actattggta accagaccgg cattttacgt
    18001 aatgaaccag gcatcctttc tcccacaaat atctagactt aagtaaagcg tggagtgtac
    18061 tggatatacc caatgctggt tgagcatttg ttgaaaaaat tttcccccgt tttgactaaa
    18121 atgcgccagg attgatggaa tcattagtct ggtgattagg aataatctgg atgaatgaca
    18181 gggaaaacat gcgtaatact tacgcagttc tctgaaaaag tgatttaaat ttagatggat
    18241 agcggtgtat ggaaacgttc tgttacatga aatggcccgt tagacatcac aaatcgcgaa
    18301 gagtttccca ttaatttttg atatatttaa aacttaggac ttatttgaat cacatttgag
    18361 gtggttatga aaaaaattgc atgtctttca gcactggccg cagttctggc tttcaccgca
    18421 ggtacttccg tagctgcgac ttctaccgta actggcggtt acgcacagag cgacgctcag
    18481 ggccaaatga acaaaatggg cggtttcaac ctgaaatacc gctatgaaga agacaacagc
    18541 ccgctgggtg tgatcggttc attcacttac accgagaaaa gccgtactgc aagctctggt
    18601 gactacaaca aaaaccagta ctacggcatc actgctggtc cggcttaccg cattaacgac
    18661 tgggcaagca tctacggtgt agtgggtgtg ggttatggta aattccagac cactgaatac
    18721 ccgacctaca aacacgacac cagcgactac ggtttctcct acggtgctgg tctgcagttc
    18781 aacccgatgg aaaacgttgc tctggacttc tcttacgagc agagccgtat tcgtagcgtt
    18841 gacgtaggca cctggattgc cggtgttggt taccgcttct aatcactttg gtgatataaa
    18901 aaatccgcct ctcggggcgg atttttgttt ttaaggcttc gggtcgaaaa tatcggttcc
    18961 gagatggttg taatccacct tctgtaactg gaagttggtg atgtatatcg gcccggcttt
    19021 cttctcagat ataaacgggt atttgatgtt aatttccttc gctttaattc ccgtccactg
    19081 cgagaaaaag cctaagaagt cattggctga gcggcgggct ttaatcaccc gatgcgcttt
    19141 atcgtcgctg gaaatgacca taaaaggcac ctggaaattt tgctgatact tatcatcatg
    19201 ggcaaggtat tgcacgtctt tgccgcgctc tttaaaggcc agaccgtggt cagaaaagta
    19261 aaccagcgag aagctgctgc cgctgttgcg taactgatcg tacagcttgc gcagtaaatc
    19321 gtccgtttgc gtcatagtat agagatagca cgacgtttct ttcgattgca caaaggtttc
    19381 gtatttgcct tttgtccggt cgcaggcctg cggatgcgag cccatcagat gcagaacaat
    19441 cagctgcggt tgcgagtgct cttgcgccag cacttgagcg gtcatatcca gtaacgcttc
    19501 gtctttcgtg tttttatctg cttcaaaatt accttctttc aggaagtaca cttcatctgc
    19561 acgtttggcg atgctggcga tagcagtatc gtattcgccg atttggccct ggttggaaaa
    19621 ccaccaggtc tggaagcccg cgcgatttgc cagggtgaca aagttatcct gaaactgtgg
    19681 tttgccatcg actacgcgat tgagcgttaa gccaagcgat ttctgcgtgg agccactggc
    19741 ggcaatgtag tcagcaaata tcaaaccgtt aacgctgctg gcaaacgggg tattgtccca
    19801 gtgaccgcca aaggcaccga gggcatcgcg acgcgcgctt tcaccgatca ccaccacata
    19861 ggtctgatac ttcggcttaa cggcagtgac cgtccaggta tctttcattc cggaaagttt
    19921 tgccatccgt tcttgctctt caatcacctc gttattattg acgatgacat ccttagcaaa
    19981 acgaaatacc ggatagccag tatctttcag tttaaatacg ccaccccagg ccaggttttg
    20041 cacgggagca acaaaaaatg tcgccacact gaataccaga cacaggctgt cgaaggtatt
    20101 ccagcgcgct ttttcattct ctttttttcg cctgattgct attacgccga gcgcaaaaat
    20161 aaataagccg accaggtagc tgtaccacgg aaaaatcgtc aggatttcgg tcgactcttc
    20221 catattggtg gagtgcaatg ccagcaatgt attgaaatta ggcgcgccgt aggcctgagc
    20281 aaaagggaaa taacaagccg ccaccagcga actgacaccg accagaactt tttgtacgcg
    20341 aggcaatgtt cgccatagca aaagcaaaat agccgtaaac gcagcggtgt agagcaaact
    20401 gaaggggtaa cctaagccga ggttaattaa tagcgactgt aaaaagtaga acgcagtcca
    20461 cgggctaaat acccggctac gggtaacaag cgattctttg agggttaaat tcatatgcca
    20521 ctatcgtaac aaacgccatg tgcttaccct ggcgtcaagg gtcaatacct gcaagagtgc
    20581 gtgaagaggg gaaaggggtc cgcatccgcg agccgcaata tacgcagggc tgcaagaaga
    20641 tagagcgagc cgcagttcag gtcaactact gacgaacaaa tcttttgcga aggggattgc
    20701 aaatccgaca aaaagtgggg tttttcagac caaaacgcgt cgtgcctgca tgaaagcagg
    20761 agtgaagcgg cgtaccgcga cgccgctatt tatcggaagg tttatcttgc tgcggtttgt
    20821 tgttgaccat atcgcacaac atagagagca gcattaaccg tactttaaag ggagaatgac
    20881 taaacacgcg catacacctc ttgaactcat tcataagacc tcctgacttg ctaatcccgt
    20941 cgatccttga gggatgattg cattacatac agatatagca caggctatat tatatagcta
    21001 ttgctaaaac gttaattttt tgtaccttcg caactctggt ttacaatgtg cgcacgaaat
    21061 gagaacgcta tgtatgcgtc accataatga ggaagcacaa tgagtcgtcg cgcaggtacg
    21121 ccaacagcaa aaaaagtgac gcagttagtg aacgaggaag agcacgttga agggttccgc
    21181 caggtcagag aggcgcatcg gcgcgagctt attgatgatt acgttgagct gatttctgac
    21241 ttgatcaggg aagtggggga agctcgtcag gtagacatgg ctgctcgttt gggagtttcg
    21301 caaccgacgg tggctaaaat gcttaagcga ctggcaacta tggggctgat tgaaatgatc
    21361 ccctggcgtg gcgtgttttt aacggcagaa ggagagaagc tggcgcagga aagccgcgag
    21421 cgacatcaga tagtcgaaaa tttcttgttg gtgttgggcg tcagtccgga aatcgcccgt
    21481 cgcgacgcgg aaggcatgga gcaccatgtt agtgaagaga cgctggatgc ctttcgtttg
    21541 tttacccaga aacacggtgc caaatgagcc tgcctttttt acgcacgctg caaggcgatc
    21601 gtttttttca gttattaatt cttgttggta tcggattaag ttttttcgtg ccctttgcac
    21661 cgaaatcctg gcctgctgct atcgactggc acaccatcat caccttaagc ggcctgatgc
    21721 tgctgaccaa aggtgtggag ttaagcggtt attttgatgt gctggggcgc aaaatggtgc
    21781 gccgctttgc tacggagcgt cggctggcga tgtttatggt gctggcggcg gcgctgcttt
    21841 ctacctttct gaccaacgat gtcacgctgt ttattgttgt tccgctgact atcacgctaa
    21901 aaaggctgtg tgagatcccg gttaatcggc tgattatttt tgaggcgctg gcagtcaacg
    21961 ctggttcgct actgacgcca attggcaacc cgcaaaatat tcttatctgg ggacgttctg
    22021 gtctttcgtt tgccggattt attgcccaaa tggcaccgct ggctggcgca atgatgctga
    22081 cgctcctgct gttgtgctgg tgttgtttcc ctggaaaggc actccaatac catacggggg
    22141 tgcaaacacc ggagtggaaa ccgcggctgg tgtggagttg tctggggctg tatatcgtct
    22201 ttctgacggc gctggagttc aaacaagagc tgtggggact ggtgattgtg gcggcgggct
    22261 ttgcgctgct ggcgcgtcgc gtggtgttga gtgtggactg gacgctgctg ctggtgttta
    22321 tggcgatgtt tatcgacgtc catttactga cccagcttcc agcgttgcaa ggcgtgttgg
    22381 gtaacgtgag tcatttatct gaacccggat tatggttaac ggcaatcggt ttatcgcagg
    22441 tgatcagtaa cgtgccgagt actatattgt tgctgaacta tgtgccgccg tctttattac
    22501 tggcatgggc ggtaaacgta ggtggctttg ggttattacc cggatcgctg gcaaatttga
    22561 ttgcgctacg tatggcgaac gatcgccgca tctggtggcg tttccatctc tattcaatac
    22621 cgatgctgtt gtgggcggcg ctggtgggat atgttttgtt ggttatgatc ccggcctggt
    22681 aaggtctggc aaataaaaaa ggcggattat gagtccgcct ttgttttacg ttttgaagat
    22741 taattcagac gaaccggcat cccggagcgg tttttaatcg cttcatcaag aacaacctgg
    22801 tcaacatctg gctggccggt cactgtctgc acgctcttcg tcagggtaat tggcacaatt
    22861 tcctgacctt caaattgggc ttcggtggta gacagcgggt tatggacttc aatgtaacgg
    22921 ctgccgtctg gctcggtggt cgcttttacc ggctcatcaa taaactgtac gcgggtaccg
    22981 accggtactt tctcgaacag gaatttgatg tcttcgttac gcagacgcac acaaccatga
    23041 cttacacgca gaccgatacc gaagttagcg ttggtgccat ggatagcgta caggcgaccg
    23101 atgtagagtg catacagccc catcgggtta tccggacctg ccggaacgac agccggaagc
    23161 ggttcgcccg cagcgcggta ctctgcgtgc attttggcgg tcggcgtcca ggtcgggcct
    23221 gctttcttac gctcaacttt ggtggtccag ttgataggcg tatctttgcc taactgacca
    23281 atgccgatcg gcagcacgat aacggtgttg gtccctttcg gatagtaata cagacgcatc
    23341 tctgcactgt taatgacgat gccttcatga acggtatccg gcaggatcag ctgctgcgga
    23401 atgttcagta cagtaccgcc tttcggcagg aaggtatcca cacccgggtt cgcttccatc
    23461 atattggaaa gccccatctg gtactccgcg gcaaaatact ccagcggctg agtgttacct
    23521 tcaggaatgg tgatcacctg attctgacca accaggcgac tcccgtcggt tggcagagga
    23581 taagttaccg cagaggcggt actgcaaaag ccgacaacag cgaaggccgc tgcgaataat
    23641 gttttcaatt tcatattcat gttaagcgag attttgtgcc tggcaggcca ttgggttgag
    23701 aatattagag tattggaagc gcattataag ttcattccag ctcacagtga aatcagatgt
    23761 gtacgaaatc acattttttg cctttggctt gagtgtagat cttaagcgag gagcaggatc
    23821 ttctttcaga cttatggcat aatgcgcggt ttgtcatatc tcttttcagg atacgcctgt
    23881 gttagtttcc agtaacgtca ccatgcagtt cggcagtaag ccgttgtttg aaaacatttc
    23941 cgtcaaattt ggcggcggca accgttacgg cctgattggc gcgaacggta gtggtaaatc
    24001 cacctttatg aagatcctcg gcggcgacct tgagccgacg ctgggtaacg tttccctcga
    24061 tcccaacgag cgcattggta aactgcgtca ggatcagttt gcctttgaag agttcactgt
    24121 gctggatacg gtgatcatgg ggcataaaga gttgtgggaa gtgaagcagg agcgcgaccg
    24181 catctatgct ttgccggaaa tgagtgaaga agacggctat aaagtggccg atctggaagt
    24241 taaatacggc gaaatggacg gttactctgc ggaagctcgt gccggtgaat tgttgcttgg
    24301 cgtgggaatt ccagtggaac agcactacgg cccgatgagt gaagttgctc ctggctggaa
    24361 gctgcgtgtg ttgctggcgc aggcgctgtt tgctgatccg gatattctcc tgctcgacga
    24421 accgaccaac aacctcgaca tcgacaccat tcgctggctg gaacaggtgc tgaacgagcg
    24481 tgacagcacc atgatcatca tctcgcacga ccgtcacttc cttaacatgg tctgtaccca
    24541 catggcggat ctggattacg gcgagctgcg cgtttatccg ggtaactacg atgagtacat
    24601 gacggcggcg acccaggcgc gtgaacgtct gctggccgat aacgccaaga agaaagcgca
    24661 gattgctgag ctgcaatctt tcgttagccg ctttagcgcc aacgcctcga aatctcgcca
    24721 ggcaacttcg cgcgcgcgcc agattgataa aatcaaactg gaagaggtga aagcctccag
    24781 ccgtcagaac ccgttcatcc gttttgaaca ggataagaag ctgttccgta acgcgctgga
    24841 agtggaaggt ctgaccaaag ggtttgataa cggtccgctg tttaaaaatc tcaacctgct
    24901 gctggaagtc ggtgaaaaac tggcggtact gggtaccaac ggcgtcggta aatcaacgct
    24961 gttgaaaacg ctggtgggcg atctgcaacc ggacagcggc accgtaaaat ggtctgaaaa
    25021 cgcgcgcatt ggttactatg ctcaggacca cgaatatgag tttgaaaatg atctgaccgt
    25081 gttcgaatgg atgagccagt ggaagcagga aggcgatgac gagcaggcgg tgcgcagtat
    25141 tctcggtcgt ttgctgttca gccaggacga catcaaaaag cctgctaaag tgctttccgg
    25201 tggagaaaaa gggcggatgc tttttggtaa gttaatgatg cagaagccga acattctgat
    25261 catggacgaa ccgaccaacc acctggatat ggaatccatc gagtcactga acatggcgct
    25321 ggaactgtat cagggcacgc tgatctttgt ttcacacgac cgtgagttcg taagctccct
    25381 ggcgacccgc attctggaaa tcaccccgga acgcgtgatc gactttagcg gtaattacga
    25441 agattacctg cgtagtaaag ggatcgagta agttattatc cgtcaccccg atactgtcgg
    25501 ggtgacggcg gctgatgaca aacgcaagat tgcctgatgc gctatgctta tcaggcatta
    25561 agaaagcgca cttgtcagca atctgagtaa cgtcgaatcg attttcttaa tcccacatca
    25621 attcacttca attaactctc aaacatccat gcccaacgct cgcttaccgt gaatgttgag
    25681 gtcggcaagc gtaaagcgtc cttcccagtt tgtttcatag tcctcgcgcg ggaagtcgcc
    25741 cggcgatgcg cctttttcca gtgcggcctt caccttgtgc gcgtaggcaa gatttttctc
    25801 gcacatcggt gccgcaggaa tgtacatcac gttgccccaa ccctgttgat tttcaacggg
    25861 ggcaacggaa tggatgacgt cgcagtgcca ccagacggag tctccggcct cgagttttgg
    25921 aatgctggtt aacgcctcaa ttaacagtgg atgccattgc tctgataccg gcaacactct
    25981 tccgggcgct acgccgcaca gttcatcctc cggcacatca tcaagcagcg gacgtaacag
    26041 tacgtacgcc atggcttcag gaatgggcac gacgtgcagc aacccctgac taggcagcat
    26101 atcagagagc gctgtccagc cctggaatgt ccgaaacacg gaacatttgg tggtgttgtc
    26161 caccgtgtac tcttcaactt ccgtacgatg tgccgcatgc cagggatcgt atttcgccag
    26221 attgccatta aagacgttag cgaaaacgtg ctgatacgct ggaagcagcc agcgttccag
    26281 cgccccggag tcggtatgcg ctccaagacc tttggaggtc gttccgggcg gacggcggcg
    26341 gatacggtca gggtagatga cgctcacatc cgggttaaac cattgctttc catcactttc
    26401 aaatgtccac agacgattga gaaacgactg cgcattcgcc atttcttcac tctggcgggc
    26461 ctgcatttgc gcctgcgacc agtagatggg gtaaatctcg ggacgtgaag cgctgagcgt
    26521 cccgaagaaa ttatcgccgg ggcctttgta gacctcgtca aagcggttgc ggtccagata
    26581 gtccagcatc gactgatccc agcctagcgc ttgttcgcgg gggaaatggc cttttatcac
    26641 cgcacaaccg cgacgtttaa tctgttcgcg ctgctctgca gtaacatgac ctgctttgat
    26701 atcggcataa gacagcaccg gccagacggc atcgccctgt gctttgagtg cgttgatttc
    26761 agccactcgc gtggcaatgt catcgcttag ctgattaaag atctgctgga cgtcgccaag
    26821 ctgcgcccgc agcgcgtgct tcatctgacg gatagctgct ttgtgatcgg caggcaatgt
    26881 gtcgctggta aaagtagaag ccataaccac ctcgcattaa ctttcattcg aaactatgct
    26941 gatttgcatg tgacaatata agttataaat aagttaatgc aagtttaatg atttgatgtg
    27001 atgtacagga tgtaaaaaga agggataacc ggagcgcatg gctccggtag agaggtcagc
    27061 tgttaaaagg ggatgtgtta tccagtacag cctgaatcac attcagcgcg ccttcatgat
    27121 tattatcatc ggtagcgtaa cgggcgattt gtttaatgtt ttccgcagca ttgcccatcg
    27181 caaaggaata acgcgccatt ttcagcatct ccgcatcgtt accgctgtcg ccaatcgcta
    27241 ccacattttg cggtgacaga tcccagcgtt tcagtaaccg cgaaataccg tttgctttat
    27301 gtagaccggg aataatcagg tcgataaagc caaaaccact ggtgacgggt ttcataatgc
    27361 catcgagcgc tatgtgcagt ttgtcgatca ctaacgggat ttgttcatcc ggcaggttga
    27421 gtgaaaactt gaacagtacg tcgtcaatct cctgataatc ttttacaggt ttcaggcgat
    27481 ggtagtgttt tgccatcagt gcgacaaatg cttcgggggc attttcgctg acatatgcac
    27541 tttgcagacc gcaggcgaca aaattgagtt gcttatcttt tagcaactcg ccaataacaa
    27601 tccgcgattc atgtcgggtc agttcgccgt ggaacagttg cttgccatgt tcgtaaacca
    27661 gtgcgccgtt ttccgcgaca aaagagatct catcctttag ctcaggaaag aatgaaataa
    27721 gctggtaata ctgattaccg ctggcgacaa cgaactcaat gccgcgcttt ttcagttcct
    27781 gatattgcgc cataaaacgt ggttggttgt acgttttgac gtcgttaaga aaagtaccgt
    27841 ccatatctgt gacgataact tttacgctca taggtgtgct cccggctcga aaatgaaacc
    27901 gtaacagtgt aataacaatg tgacgcagag cacaaattat atttcgaatg aaagtaagga
    27961 tgaaattgat gatgtgaatg atttagcccg gcgacgtctc cgccgggctg aggggattac
    28021 agcatatgtt cggtacgggc gatgatatcg tcctgagcgt ctggagagag cgcggtgaag
    28081 aaggcggaat aacccgcgac acgcactacc agatcgcgat actgatcggg atgttttttc
    28141 gcctccagca gcgtttcgcg ggaaacgatg ttgtactgaa tatgccagcc tttatgcact
    28201 tcaaagaagg tgcgcagcag gatcatcagt ttctgcttgt cagattcgtt ctccagtgtt
    28261 gctggattca gtttctggtt gagcaacacc ccgccgagaa tcgctgccgt aggcagttta
    28321 cccaccgagc caatgaccgc agtagggcca agatgatcag taccggaagc cgggcttgcg
    28381 ccttctgcca gcggggtgtg ggctttacgt ccgtctggcg ttgccatagt ttgcgcgcca
    28441 aacggtacgt tagcggagat agacgacgta cccgcgtaat agttgccgcc aaccggacca
    28501 cgaccgtagc gcgggttatg gtactgtttc agttcgtcga tataggtctg ataagcgcga
    28561 gccagcagcg tatcgacagt atcatcgtcg ttgccgtact tcggcgcacc gttaatcagc
    28621 cgctgacgca gctgctcgtg agtcaggccg tcgaagtcat tcgccagtgc ggcagccagc
    28681 tgttgctgac taatcgcgcc ctgttcaaat accagtttct tcacggccgc caggctgttg
    28741 ccgaggttgg caatgccaac ctgcaggcca gaaacccagt catatttcgc gccgccttgc
    28801 ttgatacttt tcgctcgctc aatacagtca tccaccagcg ccgagcagag aatatcgtgc
    28861 acgttctctt ccagcatggt gtcgacgaca tattcgattt cgattgattt acgcgtgtag
    28921 taacggattt gcgtatccca cgcgtccatc acttcatcga agttgttgaa gttacctgcc
    28981 gacaacgctt tttcttgtgg caggaacact ttgccgctgg tggcatcacg cccgccttcc
    29041 agcgccgcca gcatcacgcg ggcgaagttg ataaagctca tgccggtaca gcgatagccc
    29101 catttgccac cgacggcggt ttcgatacaa ccaatcgctg catagttgta agcatcctgc
    29161 ggttcaatac cgagtttaat aaattccggg atgacgattt cgtcgttgtt gaacgccggc
    29221 atcccgaagc cgcaacggat cacctgtacg caggcgtcga ggaaatcgtt gctcattccc
    29281 gcgtggtaac gcacgctgag gttaggctga gtggaacgca ggcgaccgca ggattcgagg
    29341 atcgcgtaag agagtggatt caccgcgtcc attggttgac catcaaccag attttgtccg
    29401 ccaatggtga cgttctgata cagaggactt cccgcagagg cttttgagtg tgagcctgag
    29461 cggattttgt tcacttccag cagtttcagc cagcagctat gcagcaattc gatggcgtgt
    29521 tcgcgatcca gtgtctggtt gagttcaacg tcgcggcgat agtacgggta gagatactgg
    29581 tccatacgac caaacgatac tgagtgaccg ttagattcga tctgcaaaat caactggatg
    29641 aagtaacaca gttgcagcgc ctgccagaaa gtctgcggcg gctggtgggc gataagatcg
    29701 cagttttctg ccatcgccag cagttcatcg cgacggcttt cgcgggtttc ggtcgcggcc
    29761 attgcacgcg ccagggcagc gaaacgttca atgtgttcac tgactgccac cagcacgata
    29821 tcaatcgctt tcaggaactg ttcgccgtgc agatcttcca gtaccgtcag attgatgcgc
    29881 gagcgacgtt ccgctacttt ctcgcgcaga ccatcaagcc tttttccagc agcagcggga
    29941 aattcaccgc caggtgcgca tcgccggagg tcatattgcc ttccgcttta atgattccgg
    30001 tcgccagcag acctttttgc tcatcggtaa acatgccgta gcagcgatcc tgtaccgtct
    30061 gaccgcgcca ccacgggcac acttcatgca gaacgcgttt gttctcttcg ctcaccgcaa
    30121 agccagcacc gggacgatct gccagatcat caatctcttt ttcgatccac gagacagtat
    30181 attccgggaa gatcggcgcg gcgcgaactt cgcttgcctg gttgccgatg atcaactcat
    30241 cgtgcttgat ccagatagtg cgattcgcca ggtgatgtgc cagcgccagc gcgcgacgta
    30301 cagggatcgg cttatcgaga tgttgttgat acatctcggt atagtgctgc gcgcgctcgg
    30361 tacaaaccgg cggtttcaca atatgcacca gcgcattttt gtgcgcttta atgcggtcgc
    30421 tgagcgtgtc cagtttcagt gtggtcatgg ttgttatcct cgtaaggtcg cggttaaccc
    30481 tttctggcag gcatactgct gggcaaagtc gagcagttct ggcgcatcaa gcggtttttc
    30541 cggggcgtca tagggcagat taagtaagtg atatttgttg atgcccagcg tgtggtaggg
    30601 caggaaatga atttcgccaa cgtgcagctc gtcggcggca aaatcggtaa tggctttcac
    30661 agaggtttcg tcggcattaa agccttgaat cagcggcacg cggataatga tttttttgcc
    30721 cgctgcggcg agttttttca ggttatccag caccctggcg gcgttaccgt cggtccactg
    30781 tttaaacggc gcgtcggcaa cgtgttttaa atcggcaaga aacagatcga tatagggcag
    30841 agaaggggcg atatatttcc acggcacatg caggcaggtt tctaccgcag tatgaatgcc
    30901 ggcctcgtgg ctggcttgca gtagcgccgt cgccatttcc ggctgcatga atggttcacc
    30961 gccagaaagt gttaagccgc cgccgctgcg atcgtaaaac ggtttatcgc gcagaacggt
    31021 caccatgatc tcctcaacgc ttttcacttc cccacacacg gttaacgact gtgtcgggca
    31081 gcagtcggtt aacgccgtca gatgctccgg ggttaacttt tcccgatgaa taagcaaacc
    31141 attcagcgcg cgctcaatca cttccggcgc ggtcttagcg cacagctcac agccttccag
    31201 gcacagacgt gggtcataca gcagatcctg cgtgcgggcg cggctttccg ggttctgaca
    31261 ccagcggcag cccagcgaac agcctttaag aaatacgacc gtgcggatac cggggccatc
    31321 atgggtcgag tagcgctgaa tattgaaaat catagttgcc tctctgtttc gttcaaatat
    31381 taaaatactt tcgaatgaaa gttagattga tgtgcgtcaa ctgttcagag agttttcccg
    31441 tgatagtcta cattcagaca aaaagtacat tttgaggatg gttatggaac tgtatctgga
    31501 tacttcagac gttgttgcgg tgaaggcgct gtcacgtatt tttccgctgg cgggtgtgac
    31561 cactaaccca agcattatcg ccgcgggtaa aaaaccgctg gaggttgtgc ttccggaact
    31621 tcatgaagcg atgggcggtc aggggcgtct gtttgcccag gtaatggcta ccactgctga
    31681 agggatggtg aatgacgcac gtaaattgcg ttctattatt gcggatatcg tggtgaaagt
    31741 tccggtgacc accgaggggc tggcagctat taagatgtta aaagcggaag ggattccgac
    31801 gctgggaacc gcggtatatg gcgcagcaca agggctgctg tcggcgctgg caggtgcgga
    31861 atatgttgcg ccttacgtta atcgtattga tgctcagggc ggtagcggta ttcagactgt
    31921 gaccgactta caccagttat tgaaaatgca tgcgccgcgg gcgaaagtgt tagccgcgag
    31981 tttcaaaacc ccgcgtcagg cgctggactg cttactggca ggatgtgaat caattactct
    32041 gccactggat gtggcacaac agatgattag ctatccggcg gttgatgccg ctgtggcgaa
    32101 gtttgagcag gactggcagg gagcgtttgg cagaacgtcg atttaaccgg acgttctgca
    32161 tcctcataaa ttgctgatga cgtggcggag tgcaccgatg cctgccgcgc ttggaaatct
    32221 gtgcccgaac cgtaggccag ataaggcgtt cacgccgcat ctggcgtctg gagcaaatgc
    32281 ctgatgcgac gcttgtcgcg tcttatcagg cctggaggtg gcaattactg cccgcacacc
    32341 tcacaccccg gattgcgcat cagtttcatt tcgcgaaact gacaggtcat cgcatcgtac
    32401 atgacgattt tcccgctggc cggtttgcca taacctgcca gcagtttgat cgcttccatt
    32461 gcctgcaacg aaccaatcac gccgatcaac ggtgccatca cgcctgcttc tacgcaggtt
    32521 aacgcgttct cgctaaacaa acggctaagg cagcgatagc acggttcgcc gtcctgataa
    32581 gtgaagaccg tgatttgccc ttccatacga attgccgcgc cggaaaccag cggcaccttc
    32641 gcggcaaaac agcctgcgtt cagttgatta cgtaccgcaa cgttatccgt acagtcgagc
    32701 acaagattgt gttcagcaat caatgctgca agttctgcgt catccagcag tgcattgact
    32761 ggcgtaatcg cgatatgtgg gttgatccgc gtcagagcgt cacgggcgga ttccaccttc
    32821 ggttgcccga ccgtagcatc actgtgcagc gtctggcgtt gcagattcga gagcgaaacc
    32881 gagtcgaagt cgagcagcgt caggttgccg acaccggcgc ttgccagata ctgcgaagct
    32941 gcgcagccga gtccaccgag gccgacgacc agcacacgag aatctttcag cgcctcctga
    33001 ccgtcaaaat caaaaccgcg cagaatgatt tgccggttgt agcgcagcat ctcttgatcg
    33061 ctgagttccg ccattacagg cctccgaaca acgcgttaaa cggttctact tccacccatt
    33121 cgcccacttc cacattgccg cgatcgcgtt ccagcacgat aaagcagttg ccgaggctaa
    33181 aggagctgaa tatatgtgaa ccctgatgtc cggtggtcgt cacttccagt tcgccatcgg
    33241 cgttgcggtg cagcacgccg cgctggaaat caagacgtcc tggcgttttt ttcaggcggg
    33301 acgctgtgcg tacgcgctgg cgcgcgggca ggccgctggc ggtgttgccg cttagttttg
    33361 ccagcaaagg ctgtaccagt tgatagaagg tcagcgtcgc tgaaaccggg ttgcccggca
    33421 ggccgcagaa ccagctattg ctgagtttac cgaacgcgaa cggtttacct ggtttaatcg
    33481 ccagcttcca gaaggcgatc tcccccagct cttcaagaat cgttttggtg taatccgctt
    33541 cacccactga aacaccgccg gaactgatca ccacatccgc ctggctgtcg gcttcaataa
    33601 atgcggcgcg cagggcatgt ggatcgtcgc ggataatccc taagttgatt acctcgcaac
    33661 ccagctgttc taacatcagg tgtacggcga gacggttggt atcgtagatt tggccgtcgc
    33721 ccagcggctg gcctggcaac tggagttcat caccggtaga aaaaagcgct acacgcactt
    33781 tacgaatcac cggaacttcg gcaatcccca gtgaagcaat tactggcagc tctgcggtag
    33841 tcaggcgagt ccccgccggg aaaacaaccg cacctgcaga gatatcttca ccgcgacggc
    33901 gaatattttg cccgctacgc acttcagcag taaaacgcac gccattgtcc gtttgttcag
    33961 tctgctcctg catcaccacc gcttcgcagc cttccggcac cggcgcaccg gtcataatac
    34021 gaatgcaggt acccgcaggc cattcaccat ggtacggctg accggcaaag gatttaccgg
    34081 caacgggcag cggttgtccg gaggtgatat cggccaaacg taccgcgtaa ccatccattg
    34141 cggagttatc aaaccctggg acatccagcg gcgaaacgac atcgctcgcc agaatacgac
    34201 caaaacactg aaccagtggc agcgtttcct gggcggtcag tggggtgacg cgagaaagca
    34261 tctcattaag cgcggtgtcg agcgacatca atccggtggt aaattccatg aaaacactcc
    34321 tgcggaggca aaatcgaatt tgcctattat gtcagaaaaa cgccacagac tgtatgccac
    34381 ctcgggcgta gcgctgggtc ctgcctttac atgccatatc catctttcta tattcaaaaa
    34441 ttgaatgagt aattcataaa aattctgata tttatagcaa aagtggcgag ccacccttaa
    34501 tggacgaata ctatgggcaa agcagtcatt gcaattcatg gtggcgcagg tgcaattagc
    34561 cgcgcgcaga tgagtctgca acaggaatta cgctacatcg aggcgttgtc tgccattgtt
    34621 gaaaccgggc agaaaatgct ggaagcgggc gaaagtgcgc tggatgtggt gacggaagcg
    34681 gtgcgtctgc tggaagagtg tccactgttt aacgccggaa ttggcgccgt ctttacgcgt
    34741 gatgaaacgc atgaactgga cgcctgtgtg atggatggta acaccctgaa agccggtgcg
    34801 gtggcgggcg ttagtcatct gcgtaatccg gttcttgccg cccggctggt gatggagtaa
    34861 agcccgcatg tgatgatgat tggcgaaggg gcagaaaatt ttgcgtttgc tcatggcatg
    34921 gagcgcgtct caccggagat tttctccacg cctttgcgtt atgaacaact aatggcagcg
    34981 cgcgaggaag gggcaacagt cctcgaccat agcggtgcgc cactggatga aaaacagaaa
    35041 atgggcaccg tgggggccgt ggcgttggat ttagacggca atctggcggc agccacgtcc
    35101 acgggcggaa tgaccaataa attacccgga cgagttggcg atagcccctt agtgggtgcc
    35161 ggatgctacg ccaataacgc cagtgtggcg gtttcttgta ccggcacggg cgaagtcttc
    35221 atccgcgcgc tggcggcata tgacatcgcc gcgttaatgg attacggcgg attaagtctc
    35281 gcggaagcct gcgagcgggt agtaatggaa aaactccctg cgcttggcgg tagcggtggc
    35341 ttaatcgcta tcgaccatga agggaatgtc gcgctaccgt ttaacaccga aggaatgtat
    35401 cgcgcctggg gctacgcagg cgatacgcca accaccggta tctaccgtga aaaaggggac
    35461 accgttgcca cacagtgatg aacttgatgc cggtaatgtg ctggcggttg aaaatcttaa
    35521 tattgccttt atgcaggacc agcagaaaat agctgcggtc cgcaatctct cttttagtct
    35581 gcaacgcggt gagacgctgg caattgttgg cgaatccggc tccggtaagt cagtgactgc
    35641 gctggcattg atgcgtctgt tggaacaggc gggcggttta gtgcagtgcg ataaaatgct
    35701 gttgcggcgg cgcagtcgtg atgtgattga acttagcgag cagagcgctg cacaaatgcg
    35761 ccatgtgcgc ggtgcggata tggcgatgat atttcaggag ccgatgacat cgctgaaccc
    35821 agtatttact gtgggtgaac agattgccga atcaattcgt ctgcatcaga acgccagtcg
    35881 tgaagaagcg atggtcgagg cgaagcggat gctggatcag gtacgcattc cggaggcaca
    35941 aaccattctt tcacgttatc cgcatcaact ctctggcggg atgcgccagc gagtgatgat
    36001 tgcgatggcg ctgtcatgcc gcccggcagt gctgatagcc gatgagccaa ccaccgcgct
    36061 ggatgtcact attcaggcgc agatcctgca attaatcaaa gtattgcaaa aagagatgtc
    36121 gatgggcgtt atctttatca ctcacgatat gggcgtggtg gcagagattg ccgatcgggt
    36181 actggtgatg tatcagggcg aggcggtgga aacgggtacc gtcgaacaga tttttcatgc
    36241 accgcaacat ccctacaccc gtgcgctgtt agctgctgtt ccgcaacttg gtgcgatgaa
    36301 agggttagat tatccccgac gtttcccatt gatatcgctt gaacatccag cgaaacaggc
    36361 cccccccatc gagcagaaaa cggtggtgga tggcgaacct gttttacggg tgcgtaatct
    36421 ggtcacccgt ttccctttgc gcagcggttt gttgaatcgc gtaacgcggg aagtgcatgc
    36481 cgttgagaaa gtcagttttg atctctggcc tggcgaaacg ctatcgctgg tgggcgagtc
    36541 tggcagcggt aaatccacta ccgggcgggc gttgctgcgc ctggtcgaat cgcagggcgg
    36601 cgaaattatc tttaacggtc agcgaatcga taccttgtca cccggcaaac ttcaggcatt
    36661 gcgccgcgat attcagttta tttttcagga cccttacgct tcgctggacc cacgtcagac
    36721 catcggtgat tcgattatcg aaccgctgcg cgtacacggt ttattgccag gtaaagaagc
    36781 ggttgcacgc gttgcgtggt tgctggagcg cgtgggcctg ttacctgaac atgcctggcg
    36841 ttacccgcat gagttttccg gcggtcagcg ccagcgcatc tgcattgctc gcgcgttggc
    36901 attgaatcca aaagtgatca ttgccgacga agccgtttcg gcgctggatg tttcaattcg
    36961 cgggcagatt atcaacttgt tgctcgatct ccagcgtgat ttcggcattg cgtatctgtt
    37021 tatctcccac gatatggctg tggtagagcg gattagtcat cgtgtggcgg tgatgtatct
    37081 cgggcaaatt gttgaaattg gtccacggcg cgcggtcttc gaaaacccgc agcatcctta
    37141 tacgcgtaaa ttactggcgg cagttccggt cgctgaaccg tcccgacaac gaccgcagcg
    37201 tgtactgctg tcggacgatc ttcccagcaa tattcatctg cgtggcgaag aggtggcagc
    37261 cgtctcgttg caatgcgtcg ggccggggca ttacgtcgca caaccacaat cagaatacgc
    37321 attcatgcgt agataacatt caggcggaga ataaaatggc aagagctgta caccgtagtg
    37381 ggttagtggc gctgggcatt gcgacagcgt tgatggcatc ttgtgcattc gctgccaaag
    37441 atgtggtggt ggcggtagga tcgaatttca ccacgctcga tccgtatgac gcgaatgaca
    37501 cgttatctca ggccgtagcg aaatcgtttt accaggggct gttcggtctg gataaagaga
    37561 tgaaactgaa aaacgtgctg gcggagagtt ataccgtttc cgatgacggc attacttaca
    37621 ccgtgaaatt gcgggaagga attaaattcc aggatggcac cgatttcaac gcagcggcgg
    37681 taaaagcgaa tctggaccgg gccagcgatc cggcgaatca tcttaaacgc tataacctgt
    37741 ataagaatat tgctaaaacg gaagcgatcg atccgacaac ggtaaagatt accctcaaac
    37801 agccgttctc agcgtttatt aatattcttg cccatccggc gaccgcgatg atttcaccgg
    37861 cagcgctgga aaaatatggc aaggagattg gttttcatcc ggtgggaacc ggaccgtatg
    37921 aactggatac ctggaatcag accgattttg tgaaggtgaa aaaattcgcg ggttactggc
    37981 agccaggatt gcccaaactg gacagcataa cctggcgtcc ggtggcggat aacaacaccc
    38041 gcgcggcaat gctgcaaacc ggtgaagcgc agtttgcttt ccccattcct tacgagcagg
    38101 ccgcactgct ggagaaaaac aaaaatatcg agttgatggc cagtccgtca attatgcagc
    38161 gttatatcag tatgaacgtg acgcaaaagc cattcgataa cccgaaggtc cgtgaggcgc
    38221 tgaattacgc cattaaccgt ccggcgctgg tgaaagtggc cttcgcgggc tatgcaacgc
    38281 cagctactgg tgtggtaccg ccgagtatcg cctacgcgca aagttataaa ccgtggcctt
    38341 acgatccagt gaaagcgcgc gaattactga aagaggcggg atatcccaac ggtttcagta
    38401 ccacgctgtg gtcgtcacat aaccacagca ccgcgcagaa agtgctgcaa tttacccagc
    38461 agcagttagc gcaggtcggg attaaagccc aggtgactgc gatggatgcc ggacagcggg
    38521 cggcagaagt tgaaggtaaa gggcaaaaag agagcggcgt gcggatgttc tacactggct
    38581 ggtcggcttc aaccggcgaa gcggactggg cactatcgcc gttgtttgcc tcgcaaaact
    38641 ggccaccgac gctgtttaat accgcgtttt acagcaataa acaggtggat gacttcctgg
    38701 ctcaggcact gaaaactaat gatccggcgg aaaagacccg cttatataag gcggcgcagg
    38761 atatcatctg gcaagagtcg ccgtggatcc cgctggtggt agaaaaactg gtgtcggcac
    38821 acagtaaaaa cctgaccggt ttttggatca tgccagatac cggcttcagc tttgaagacg
    38881 cggatttgca ataagcaacg cagggagtgg aatgcttaat tacgttatca aacgattact
    38941 ggggttgatt ccgacgctgt ttatcgtctt ggtgctggtg tttttatttg tgcatatgct
    39001 gcccggcgat ccggcgcgat tgattgccgg gcccgaagct gatgcgcagg ttatagaact
    39061 ggtgcgtcag cagctggggt tggatcagcc gctgtatcac cagttctggc actatatcag
    39121 caatgctgtg cagggggatt ttggcctgtc gatggtgtca cgtcgtccgg ttgccgatga
    39181 gattgccagg cgctttatgc caacgctgtg gctgaccata accagtatgg tctgggcggt
    39241 gatatttggt atggcggcgg gaattatcgc cgccgtctgg cgtaaccgtt ggccggatcg
    39301 attgagtatg accattgcgg tgtcggggat ctcgtttccg gcatttgcgc tggggatgct
    39361 tttaattcag gtattctccg ttgaactggg ctggctgcct accgtgggag cagacagttg
    39421 gcagcactac attttaccct ccctgacgct cggcgcggca gtggccgccg tgatggcgcg
    39481 ctttacccgc gcgtcgtttg tcgacgtttt aagcgaagat tatatgcgta ccgcgagggc
    39541 gaaaggggtg agcgaaacct gggttgtcct caaacacggg ctacgcaacg cgatgatccc
    39601 ggtagtgacc atgatgggct tacagtttgg ctttttgctc ggtggttcca tcgtggtgga
    39661 gaaagttttc aactggcccg gacttggacg cttactcgtt gactccgtag aaatgcgcga
    39721 ttacccggtg attcaggcgg aaattctgct tttctcgctg gaatttattc ttatcaactt
    39781 agtggtggat gtgctttacg ccgccattaa cccggctatc aggtacaagt aaggatgcga
    39841 ctatttaact ggcgacgtca ggcggtgtta aacgccatgc cactggtcaa acctgaccag
    39901 gtacgtacac cgtggcatga attctggcga cgatttcgcc gtcagcatat ggcgatgacc
    39961 gccgcattat tcgttatttt attgattgtg gtggccattt ttgcacgctg gatcgctccc
    40021 tatgacgccg aaaattattt tgattatgac aatctgaata acggaccttc tttgcagcac
    40081 tggtttggcg tcgattcact ggggcgcgac attttcagcc gtgtcctggt tggtgcgcaa
    40141 atctcgctgg cggcgggcgt gtttgccgtg tttatcggtg cggcgatagg gacgttgctg
    40201 ggcttgttgg ctggatatta tgaaggctgg tgggatcggc tgatcatgcg catttgcgat
    40261 gtgctgtttg ccttcccggg tattttactg gcgatcgctg ttgttgcggt gttgggaagc
    40321 ggcattgcta acgtgattat tgcagtcgcc attttttcca tccctgcgtt tgcccgcctg
    40381 gtgcgcggca acacgctggt gttgaaacag caaaccttta ttgagtcagc acgcagtatt
    40441 ggtgccagcg atatgaccat tttgttgcgt catatcctgc ctgggaccgt ctcttctatc
    40501 gtggtgtttt tcaccatgcg cattggtacc tcgattatct ctgccgccag cctgtcattt
    40561 ctcggcctcg gtgcgcagcc gccgacacca gagtggggag caatgctcaa tgaggctcga
    40621 gcggatatgg ttatcgcgcc gcatgtcgct gtttttccgg ccctggctat ttttctgact
    40681 gtactggcgt tcaatttgtt gggcgatggt ttacgcgatg cgctggatcc gaaaattaaa
    40741 ggatagttac gtttgaatat tgcttgaaag agtaatcacc tcacaggaaa ttattgccct
    40801 aagcaagtgt tgtaactttc tgctgatttt gtagaatcgg gtaatttggt taaatagccg
    40861 cagcaaggga ctgtttttgc agcggcacag cgttcagaca gttattttgt taaatgtatt
    40921 aacatgctga gtttatacga aaagataaag ataaggctga taattttatt tttattggca
    40981 gcactgtcat ttattggcct ttttttcatc attaactatc aactggtatc ggaacgcgcg
    41041 gtaaaacgtg ccgatagccg ctttgaactt attcagaaaa acgttggcta tttctttaaa
    41101 gatattgaac gttcggccct gacattaaag gactcactgt atttattaaa aaatacagag
    41161 gagattcaac gcgctgtgat cctgaaaatg gaaatgatgc catttttaga ctcggtggga
    41221 ctggtacttg atgataataa atattatctc ttttcgcgga gggcgaacga taaaatcgtt
    41281 gtttatcatc aggaacaagt aaatggaccg cttgtcgacg agtcagggca ggttattttt
    41341 gccgatttta acccatcgaa acgaccgtgg tcggtggctt cagatgactc taacaacagc
    41401 tggaatccgg cctacaattg ctttgatcgt ccgggtaaaa aatgtatctc ttttacgcta
    41461 cgcatcaacg gcaaagatca cgatttgtta gcggtggata aaattcatgt cgatttaaac
    41521 tggcgatatc tgaacgagta tcttgatcaa atcagcgcta atgatgaagt tctatttttg
    41581 aaacaaggcc atgagatcat tgccaagaat caactcgctc gtgaaaaact gattatttat
    41641 aatagcgaag gtgattataa tattattgat tctgtcgata ctgaatatat cgaaaaaaca
    41701 tcagcggtgc caaataacgc attattcgaa atctattttt attatccggg cggtaattta
    41761 ttgaacgcat cagataaact tttttatctg ccgtttgcgt tcattattat cgtattgctg
    41821 gtggtttatt taatgaccac tcgtgtgttc cgtcggcaat tttctgaaat gacagagctg
    41881 attaatacgc tggcgttttt gcctgactca acggatcaga tcgaggctct gaaaattcgc
    41941 gaaggcgatg cgaaagagat tatcagcatc aaaaattcga tcgcggaaat gaaagatgcc
    42001 gaaattgaac ggtcaaataa attgctctca ctgatctctt acgatcagga aagtggtttt
    42061 attaaaaata tggcgattat tgagtccaac aataatcagt atctggctgt ggggatcatc
    42121 aaactgtgtg gtctggaagc cgtggaagct gtgtttggtg ttgatgaacg caataaaatc
    42181 gtcaggaaat tgtgtcagcg aattgctgag aaatatgcac aatgctgcga tatcgtgaca
    42241 ttcaatgccg atctctattt acttctgtgt cgggaaaatg tacagacatt tacccgtaaa
    42301 atagcgatgg taaacgattt tgacagcagc tttggctacc gcaatctgcg catccataag
    42361 tctgccattt gtgaaccttt gcagggggaa aacgcctgga gttacgcaga aaaactgaaa
    42421 ctggcgattt ccagtatccg tgaccatatg ttctccgagt ttattttctg tgatgacgcg
    42481 aaactcaacg aaatagaaga gaatatctgg attgcgcgta atattcgcca tgcaatggaa
    42541 attggcgaac tattcctcgt ctatcaaccg atcgttgata ttaacacccg cgccattctg
    42601 ggcgcggagg cgttgtgccg ttgggtgtct gcggagcggg ggatcatttc accgctgaag
    42661 ttcattacca ttgctgaaga tatcgggttt atcaatgagc tgggttatca gattattaaa
    42721 acggcaatgg gtgaattcag acattttagt cagcgtgcat cgctgaagga tgatttctta
    42781 ctgcatatta atgtttcgcc ctggcagtta aacgaaccgc actttcatga gcgttttacc
    42841 accatcatga aagaaaatgg cctgaaggcg aacagcctct gtgttgagat cactgaaacc
    42901 gtgatcgagc gaattaatga acatttttat ctcaatattg aacaactgcg taaacaaggg
    42961 ttacggatat cgattgatga ctttggcacc ggtttgtcaa acctgaaacg tttttatgaa
    43021 attaatccag acagcataaa ggtggactcg caatttaccg gcgatatttt cggtactgcg
    43081 ggaaaaattg tgcgcattat tttcgacctg gcacgctata accggatccc ggtgattgcg
    43141 gaaggcgtag agagcgaaga cgttgcgcgc gaattaatca aattaggatg tgttcaggct
    43201 caggggtatc tgtatcagaa acccatgcca ttctccgcct gggataaaag tggaaaatta
    43261 gtaaaagagt agtttacgta tgtccagaat caataagttc gtacttacag tcagtctgct
    43321 gatttttatc atgatttcag cagttgcctg cgggatctac actcaaatgg taaaggaacg
    43381 ggtgtatagc ctgaaacagt ccgttattga tactgctttt gcggtggcaa atattgctga
    43441 atatcggcgt agcgtggcaa ttgatcttat caacacgcta aatcccacgg aggaacagct
    43501 gttggttggc ttacgcacag cttacgccga ctcggtttca ccctcttatt tgtacgatgt
    43561 cggtccttat ctgatttcca gtgacgaatg tattcaggta aaggagttcg agaaaaatta
    43621 ttgtgcagat attatgcagg ttgtgaagta tcgacatgtc aaaaatacag ggtttatctc
    43681 ttttgacggt aaaaccttcg tctattacct ctatccggta actcacaatc gtagtctgat
    43741 atttttgctt ggtctggagc gtttttcttt actgtcaaaa tcgctggcga tggacagcga
    43801 gaacctgatg ttctctctgt ttaagaacgg taaaccggtg accggtgatg aatataatgc
    43861 taaaaacgcc atcttcaccg tttcggaagc gatggagcac ttcgcctatt tgccgaccgg
    43921 attgtacgta tttgcgtata aaaaagatgt ttatttgcgg gtctgtacat tgattatttt
    43981 ctttgccgca ttggtggcag tgatatcggg tgccagttgc ctctatctgg tacgcagagt
    44041 gattaatcgt ggtattgtgg agaaagaagc cattattaat aaccattttg aacgcgtact
    44101 ggatggcggg cttttctttt cggctgccga tgtcaaaaaa ctctacagta tgtataactc
    44161 ggcgttcctg gacgacctga ccaaagcaat gggtagaaaa tcctttgacg aagatttaaa
    44221 agcgctgccg gaaaaaggcg gttatttgtg cctgtttgac gtcgataaat tcaaaaatat
    44281 taacgacacc ttcggtcatt tgctgggcga tgaagtgttg atgaaagtgg tgaaaatcct
    44341 taaatcacag atcccggtag ataaaggtaa agtctaccgc ttcggcggtg acgaatttgc
    44401 ggtgatttat actggcggca cgctggaaga gttactatcg attctaaaag aaatcgttca
    44461 tttccaggtg ggaagcatta atttaagcac cagtatcggt gtagcgcatt caaatgaatg
    44521 tactaccgtc gaacgcttga aaatgctggc ggatgagcgg ctgtataaga gtaagaaaaa
    44581 cggcagggct cagattagct ggcagtagaa ttagaatagt gttagtaccc ctgaatcaag
    44641 gggtactaat gaatgtggta aatcatttgc aaacatttta ctttgcaaaa acttccggcc
    44701 cttttattgc caatgtattc aaaatacgta gtgccgggcc gctcggtttt atttcattac
    44761 gttcccattt cgagacactt tcttttgaca ttcccattgt atgtgccagc acggattgtg
    44821 acatgccgta tcgggtgcgg atggctttga tctcttcacc cgtcattgat ttgactttgg
    44881 ctatacgggc gctgacttca cgggctttga ccatgacggc aatggcatca acctcactat
    44941 cagacatcag gcccagtgca ttcagttcct ttgcatcttc cagcatcatt tgtaatttat
    45001 cgctcatttt tcacctcaat caagaagcca gtttcgatca aatcctctat ttgcttattg
    45061 ctaaaggcca gaaatgcatt tgccattttt ttgtaggctg ccagttcatc atcttcgatt
    45121 tctttgcttc ctttactgct tagaccactc ttg
//
LOCUS       DAZFAB010000026        45035 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZFAB010000026 DAZFAB010000000
VERSION     DAZFAB010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45035
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.026"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            258..593
                     /gene="yjdM"
                     /locus_tag="ACHJWA_001727"
     CDS             258..593
                     /gene="yjdM"
                     /locus_tag="ACHJWA_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFO4308350.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            852..1295
                     /gene="yjdN"
                     /locus_tag="ACHJWA_001728"
     CDS             852..1295
                     /gene="yjdN"
                     /locus_tag="ACHJWA_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFO4308351.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            1428..2216
                     /gene="phnC"
                     /locus_tag="ACHJWA_001729"
     CDS             1428..2216
                     /gene="phnC"
                     /locus_tag="ACHJWA_001729"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFO4308352.1"
                     /translation="MQTIICVEQLSKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMAHFAYQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRVEENAKAA"
     gene            2241..3257
                     /gene="phnD"
                     /locus_tag="ACHJWA_001730"
     CDS             2241..3257
                     /gene="phnD"
                     /locus_tag="ACHJWA_001730"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO4308353.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFSKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAMQ"
     gene            3363..4142
                     /gene="phnE"
                     /locus_tag="ACHJWA_001731"
     CDS             3363..4142
                     /gene="phnE"
                     /locus_tag="ACHJWA_001731"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFO4308354.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMLVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            4163..4888
                     /gene="phnF"
                     /locus_tag="ACHJWA_001732"
     CDS             4163..4888
                     /gene="phnF"
                     /locus_tag="ACHJWA_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFO4308355.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGIALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4889..5341
                     /gene="phnG"
                     /locus_tag="ACHJWA_001733"
     CDS             4889..5341
                     /gene="phnG"
                     /locus_tag="ACHJWA_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFO4308356.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITANYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQTRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5338..5922
                     /gene="phnH"
                     /locus_tag="ACHJWA_001734"
     CDS             5338..5922
                     /gene="phnH"
                     /locus_tag="ACHJWA_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFO4308357.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVSQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAVPRTTHVEVC"
     gene            5922..6986
                     /gene="phnI"
                     /locus_tag="ACHJWA_001735"
     CDS             5922..6986
                     /gene="phnI"
                     /locus_tag="ACHJWA_001735"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFO4308358.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDESPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            6979..7824
                     /gene="phnJ"
                     /locus_tag="ACHJWA_001736"
     CDS             6979..7824
                     /gene="phnJ"
                     /locus_tag="ACHJWA_001736"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFO4308359.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     VIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            7821..8579
                     /gene="phnK"
                     /locus_tag="ACHJWA_001737"
     CDS             7821..8579
                     /gene="phnK"
                     /locus_tag="ACHJWA_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFO4308360.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYGMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8690..9370
                     /gene="phnL"
                     /locus_tag="ACHJWA_001738"
     CDS             8690..9370
                     /gene="phnL"
                     /locus_tag="ACHJWA_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFO4308361.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWRLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            9367..10503
                     /gene="phnM"
                     /locus_tag="ACHJWA_001739"
     CDS             9367..10503
                     /gene="phnM"
                     /locus_tag="ACHJWA_001739"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFO4308362.1"
                     /translation="MIINNVKLVLENEVVNGSLEMQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHNTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAM
                     CRARQIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDQSNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            10503..11060
                     /gene="phnN"
                     /locus_tag="ACHJWA_001740"
     CDS             10503..11060
                     /gene="phnN"
                     /locus_tag="ACHJWA_001740"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFO4308363.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPICLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHHKEKHHACL"
     gene            11047..11482
                     /gene="phnO"
                     /locus_tag="ACHJWA_001741"
                     /pseudo
     CDS             11047..11482
                     /gene="phnO"
                     /locus_tag="ACHJWA_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
     gene            11484..12242
                     /gene="phnP"
                     /locus_tag="ACHJWA_001742"
     CDS             11484..12242
                     /gene="phnP"
                     /locus_tag="ACHJWA_001742"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFO4308364.1"
                     /translation="MSLTLTLTGSGGAQGVPAWGCECAACARARRSPHYRRQPCSGVV
                     KFNEAITLIDAGRHDLADRWSPGSFQQFLLTHYHMDHVQGLFPLHWGVGDPIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPREDAPRNHCDLNTVLALNQVIRSPRV
                     VLTHISHQFDAWLMENALPSGFEAGFDGMEIGVA"
     gene            complement(12319..12648)
                     /gene="yjdP"
                     /locus_tag="ACHJWA_001743"
     CDS             complement(12319..12648)
                     /gene="yjdP"
                     /locus_tag="ACHJWA_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFO4308365.1"
                     /translation="MKRHSTLFLFTLLTLTTVPAQADIIDDTIGNIQQAINDASNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQHQLNQERRQLEDEERR
                     MEDEYGQ"
     gene            complement(12717..14987)
                     /locus_tag="ACHJWA_001744"
     CDS             complement(12717..14987)
                     /locus_tag="ACHJWA_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO4308366.1"
                     /translation="MAHPGRHFFASARGRLLLLNLLVVAVTLMVSGVAVMGFRHASQM
                     QELVQQQTVDDMTGSLNLARDTANVATAAVRLSQVVGALEYKGEAERLQETQRALKSS
                     LAQLANAPLAQQEAGLVTRIITRSNELQTSVGGMLERGQRRHLERNALLSSLYQNLSY
                     LRHLQKVTHAQDDILLNEMNRLIVAAIATPAPQAIIHQLVGVMSALPTHSDTPLVNTL
                     LNDFNDFNDEMRKLAPLSAALEQSDLAISWYMFHIKALVAILNGDINRYAEQVATLSG
                     QRVAQSYQDLRSGERVIMIFALLAVVITAFAGWYIYRNLGTNLTAISRAMSRLAHGEA
                     DVTFPALQRRDELGELARAFNVFARNTASLERTSRLLKEKTTQMEIAQTERQGLEEAL
                     LHSQKLKAVGQLTGGLAHDFNNLLAVIIGSLELVNPDSPDAPRIQRALQAAERGALLT
                     QRLLAFSRKQSLHPQAVELKTLLEELSELMRHSLPATLALEIEAQSPAWPAWIDISQL
                     ENAIINLVMNARDAMEGQTGIIKIRTWNQRVTRSSGQRQDMVALEVIDHGCGMSQAVK
                     SQVFEPFFTTKQTGSGSGLGLSMVYGFVRQSGGRVEIESAPGQGTTVRLQLPRALTAA
                     KNLSPAAVEQASVSGDKLVLVLEDEAAVRQTICEQLHLLGYLTLEASSGEQALDLLAA
                     SAEIDIFISDLMLPGGMSGAEVVNAARKLYPHLTLLLISGQDLRPSHNPALPDVALLR
                     KPFTRAQLAQALGQEN"
     gene            15101..16621
                     /locus_tag="ACHJWA_001745"
     CDS             15101..16621
                     /locus_tag="ACHJWA_001745"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO4308367.1"
                     /translation="MNSTPVLEMRNIAKAFGKFYALKGVDLTVYPGEIHALMGENGAG
                     KSTLMKVLAGAYTATSGEILIDGKPFHIRTPKDALSAGITLIYQEMQLAPNLSVAENI
                     FLGSELSHGGLVQRKEMLVQAQKVIDRLGAQFNASDKVMTLTIAEQQQVEIARALHRN
                     SRILVMDEPTAALSSRETHRLFELIMRLRDEGMAIIYISHRMAEVYELSDRVSVLRDG
                     QYVGSLTRDNLNAGELVRMMVGRPLSDLFNKERDIPLGKARLNVHHLTDGGKVQPSSL
                     LVRSGEIVGLAGLVGAGRSELAQLIFGVRKATGGMIEVDGEPVVIHSPREAIDLGIGF
                     LTENRKEQGLFLEMAAAENITMATLERDARWGMLNRKKAQTISDDAIKLLNIRVPHAQ
                     VRAGGLSGGNQQKLLISRWVAIGPRILLLDEPARGVDVGAKSEIYRIMNEMARKGVAI
                     LMISSELPEIVGMSDRVYVMREGSIAGELNGKNITQENIMTLATGVNDAHSQAVTS"
     gene            16618..17592
                     /locus_tag="ACHJWA_001746"
     CDS             16618..17592
                     /locus_tag="ACHJWA_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFO4308368.1"
                     /translation="MTTSAKTSAKKMLMSDLMQTVGILPILILIVAVFGFITPNFFTE
                     SNLLNITRQASINIVLAAGMTFIILTGGIDLSVGSILGTTAVAAMVVSLSPEFAMLSI
                     PAALMLGLLLGLFNGALVAFAGLPPFIVTLGTYTALRGAAYLLADGTTVINSNINFEW
                     IGNNYLGPVPWLVVIALAVIVVCWFILRRTTLGVHIYAVGGNMQAARLTGIKVWLVLL
                     FVYGMSGLLSGLGGIMSASRLYSANGNLGMGYELDAIAAVILGGTSFVGGIGTITGTL
                     VGALIIATLNNGMTLMGVSYFWQLVIKGAMIIIAVLIDKYRTRHHQSA"
     gene            17611..18552
                     /locus_tag="ACHJWA_001747"
     CDS             17611..18552
                     /locus_tag="ACHJWA_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO4308369.1"
                     /translation="MRLKPLVTALCAGALLAAAPFAQAKELKSIGVTVGDLANPFFVQ
                     ITKGAELEARKLAGDNVKVTLVSSGYDLGQQVSQIDNFIAANVDMIILNAADSKGIGP
                     AVKRAKDAGIVVVAVDVAAEGADATITSDNTQAGEMACKYITDRLKGKGNVVIINGPP
                     VSAVQNRVEGCQTEFKRHPDIKVLSDNQNAKGSREGGLEVMTGLLAANPKIDGVFAIN
                     DPTAIGADLAAKQAQRNEFFIVGVDGSPDAEEALKRENSLFVATPAQDPQVMAAKAVE
                     IGYDILQGKPAPKEPVLIPVTMIDKNNVGNYKGWTVK"
     gene            18607..19290
                     /locus_tag="ACHJWA_001748"
     CDS             18607..19290
                     /locus_tag="ACHJWA_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HFO4308370.1"
                     /translation="MKRSAINDILGHTRQFFSQHDVHLPPFASFSPAQWQQLDTAAWE
                     EVFDLKLGWDVTAFGRNNFAAHGLTLFTLRNGSAKGMPYVKCYAEKIMHVRDAQVTPM
                     HFHWRKREDIINRGGGNLIVELWNADSNEQTADSDITVVIDGCRQKHTAGSQLRLSPG
                     ESICLPPGLYHSFWAEAGFGDVLVGEVSSVNDDDHDNHFLQPLDRYNLIDEDEPAQLV
                     LCNEYRQFR"
     gene            19305..20165
                     /locus_tag="ACHJWA_001749"
     CDS             19305..20165
                     /locus_tag="ACHJWA_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose 1,6-bisphosphate aldolase"
                     /protein_id="HFO4308371.1"
                     /translation="MPLISLAEGLAHAREHHYSLGAFNVLDSHFLRALFAAARQERSP
                     FIINIAEVHFKYISLDSLVETVKFEAARHDIPVVLNLDHGLHFESVVQALRLGFSSVM
                     FDGSTLGYEENVRQTREVVKMCHAVGVSVEAELGAVGGDEGGALYGHADESLFTDPQL
                     ARDFVDRTGIDALAVAIGNAHGKYRGEPKLDFPRLDAIRQQAAIPLVLHGGSGISDAD
                     FRRAIELGIHKINFYTGMSQAALAAVEARMAHRQPMYDEFAELLLAVEAAISDTVAQQ
                     MRIFGSAGQA"
     gene            20162..21163
                     /locus_tag="ACHJWA_001750"
     CDS             20162..21163
                     /locus_tag="ACHJWA_001750"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFO4308372.1"
                     /translation="MMERKGIIAAGNMLVDHVHQIIQWPERGWLAEITHSERSTGGAP
                     LNVLLTLAKMRTGLPLQAVGLIGQDSDGDYIMAMLEQYHVNRQHVQRTTFAPTSMSQV
                     MTDPSGQRTFFHSPGANRLLDLPAFDQLDNTMKIFHLGYLLLLDSLDMPDDVYGTRSA
                     RLLAQMRDTGFETSLDLVSRKGDPRYQPLVLPALRHLDYLVINELEAGEFSGLEIRLP
                     NGEPHIAHIAAAARELLDAGVRQRVVIHYPEGAWGETPEQGGVWIPSQKLEQREIIGS
                     VGAGDAFCAGFLYGCHERWTLTESIKLAHACARASLLCANAIDGAKTLEELQTEMSD"
     gene            complement(21160..21867)
                     /locus_tag="ACHJWA_001751"
     CDS             complement(21160..21867)
                     /locus_tag="ACHJWA_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFO4308373.1"
                     /translation="MKPVVLVVDDDTAICALLQDVLSEHVFTVSVCHTGQEAILRIEG
                     DPDIALVVLDMMLPDTNGLRVLQQIQKLRPTLPVVMLTGMGSESDVVVGLEMGADDYI
                     CKPFTPRVVVARLKAVLRRVGALAVNDEKSAGLSFNGWYLDTMRCQLHNPLQQHVELT
                     QGEYGLLLALAQNARRVLSREQLLAFTHSDSVEVFDRTIDVLIMRLRRKIELNPHQPM
                     LIKTLRGLGYVFAADVH"
     gene            22158..24143
                     /gene="yjcS"
                     /locus_tag="ACHJWA_001752"
     CDS             22158..24143
                     /gene="yjcS"
                     /locus_tag="ACHJWA_001752"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFO4308374.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKVYYRADDYKFDINAAAPETV
                     NPSLWRQSQINGISGLFKVTDKMYQVRGQDISNITFVEGEKGIIVIDPLVTPPAAKAA
                     LDLYFQHRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLPHNAQGNVGNGLGVTLATGDPSIIAPTKTIVRTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTITFIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVKALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMAVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQPQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            24352..24627
                     /gene="ytcA"
                     /locus_tag="ACHJWA_001753"
     CDS             24352..24627
                     /gene="ytcA"
                     /locus_tag="ACHJWA_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFO4308375.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRVIQRANIKLAFVGIIYTALFALYAMLFWLAFF"
     gene            24646..25677
                     /gene="mdtN"
                     /locus_tag="ACHJWA_001754"
     CDS             24646..25677
                     /gene="mdtN"
                     /locus_tag="ACHJWA_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFO4308376.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYV
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKSEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            25677..27728
                     /gene="mdtO"
                     /locus_tag="ACHJWA_001755"
     CDS             25677..27728
                     /gene="mdtO"
                     /locus_tag="ACHJWA_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFO4308377.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAITEGQCWQSDWRISESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIHQGRDLLHAWDATWNSAQALDNALQPDKAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            27725..29191
                     /gene="mdtP"
                     /locus_tag="ACHJWA_001756"
     CDS             27725..29191
                     /gene="mdtP"
                     /locus_tag="ACHJWA_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFO4308378.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVTAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGSGYQAAPVVEKK"
     gene            29389..31536
                     /gene="fdhF"
                     /locus_tag="ACHJWA_001757"
     CDS             29389..31536
                     /gene="fdhF"
                     /locus_tag="ACHJWA_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:29806..29808,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase N subunit alpha,
                     selenocysteine-containing"
                     /protein_id="HFO4308379.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLSIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            31630..32319
                     /gene="yjcO"
                     /locus_tag="ACHJWA_001758"
     CDS             31630..32319
                     /gene="yjcO"
                     /locus_tag="ACHJWA_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFO4308380.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(32497..33810)
                     /gene="gltP"
                     /locus_tag="ACHJWA_001759"
     CDS             complement(32497..33810)
                     /gene="gltP"
                     /locus_tag="ACHJWA_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFO4308381.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(34152..34748)
                     /gene="nrfG"
                     /locus_tag="ACHJWA_001760"
     CDS             complement(34152..34748)
                     /gene="nrfG"
                     /locus_tag="ACHJWA_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFO4308382.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCIGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQNPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(34745..35128)
                     /gene="nrfF"
                     /locus_tag="ACHJWA_001761"
     CDS             complement(34745..35128)
                     /gene="nrfF"
                     /locus_tag="ACHJWA_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFO4308383.1"
                     /translation="MYKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKSEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(35121..36839)
                     /gene="nrfE"
                     /locus_tag="ACHJWA_001762"
     CDS             complement(35121..36839)
                     /gene="nrfE"
                     /locus_tag="ACHJWA_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFO4308384.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWKLKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWQYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRAQLPALLAHAGILLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDV"
     gene            complement(36859..37815)
                     /gene="nrfD"
                     /locus_tag="ACHJWA_001763"
     CDS             complement(36859..37815)
                     /gene="nrfD"
                     /locus_tag="ACHJWA_001763"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFO4308385.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLSLVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAQFVH
                     RMEIPVVWGEIFLLAAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LFKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(37812..38483)
                     /gene="nrfC"
                     /locus_tag="ACHJWA_001764"
     CDS             complement(37812..38483)
                     /gene="nrfC"
                     /locus_tag="ACHJWA_001764"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFO4308386.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(38480..39046)
                     /gene="nrfB"
                     /locus_tag="ACHJWA_001765"
     CDS             complement(38480..39046)
                     /gene="nrfB"
                     /locus_tag="ACHJWA_001765"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFO4308387.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(39091..40527)
                     /gene="nrfA"
                     /locus_tag="ACHJWA_001766"
     CDS             complement(39091..40527)
                     /gene="nrfA"
                     /locus_tag="ACHJWA_001766"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFO4308388.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            40920..42878
                     /gene="acs"
                     /locus_tag="ACHJWA_001767"
     CDS             40920..42878
                     /gene="acs"
                     /locus_tag="ACHJWA_001767"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFO4308389.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINAPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLKLGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDQVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDS
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            43079..43393
                     /gene="yjcH"
                     /locus_tag="ACHJWA_001768"
     CDS             43079..43393
                     /gene="yjcH"
                     /locus_tag="ACHJWA_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFO4308390.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPVGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            43390..>45035
                     /gene="actP"
                     /locus_tag="ACHJWA_001769"
     CDS             43390..>45035
                     /gene="actP"
                     /locus_tag="ACHJWA_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFO4308391.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRNDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSITVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRA"
BASE COUNT        10360 a        12714 c        12273 g         9688 t
ORIGIN      
        1 tacgtttaaa acgtcagaaa gataaaaata tcatgtgaat aaaaaaaaga acaagtagag
       61 cattaacctt atcttaaata ataaatggag gcaaaaagat tattttcttt ttgcgtttcc
      121 tttcaaatga aaacgatcgt cgtctaaaat cagcagtacc cccgacaaac tcagggattt
      181 tgtgtataat tgcggccttt ttcggcaatc tgccgttttt tggcgctttt gccctgctga
      241 cttttgagga aatccacatg tcattaccac actgcccaaa atgcaactcc gaatacactt
      301 acgaagataa cggcatgtac atctgcccgg aatgtgccta cgaatggaac gacgcagaac
      361 ctgcacagga aagcgacgag ctgatcgtta aagatgctaa cggcaatctg ctggctgacg
      421 gcgacagcgt taccatcatt aaagatctga aggtgaaagg tagctcttcg atgctgaaaa
      481 ttggcaccaa agtgaaaaac atccgcctgg ttgaaggcga ccataacatc gattgcaaaa
      541 tcgacggttt tggtccgatg aaactgaaat ctgagtttgt gaaaaagaac tgattgtatt
      601 gtgatcggta agccggataa ggcgtttacg ccgcatccgg caacagtgcc agatgcctga
      661 tgcgacgctc gcgcgtctta tcaggcctac aaattcccgc accctccgta ggccggataa
      721 ggcgtttacg ccgcatccgg caacagtgcc gactgcctga tgcgacgctc gcgcgtctta
      781 tcaggcttcc ctctcatctg tataaatttc gaactacact taactggctt ctcttaactg
      841 aggtcaccat catgccgtta agtccctacc tctcttttgc cggtaactgc tccgacgcga
      901 ttgcctatta tcaacgtacg ttgggcgctg aactgctcta taaaatcagc ttcggcgaaa
      961 tgccaaaatc agcgcaggac agcgccgaga actgcccttc cggaatgcaa tttcccgata
     1021 ccgccatcgc ccacgccaat gtgcgcattg ccggaagcga tatcatgatg agcgatgcta
     1081 taccgtcagg aaaagccagc tactccggtt ttacgctggt gctcgattcg caacaggtcg
     1141 aagaaggaaa acgctggttt gataaccttg ccgctaacgg aaaaatcgaa atggcctggc
     1201 aggaaacttt ctgggcgcat ggctttggca aagtcaccga taaatttggc gtaccgtgga
     1261 tgattaatgt cgtcaaacaa caaccaacgc aataatccgc cgggaggccc gccctcccgc
     1321 actgtcatcg aattcccgtt aactcttcat ctgttagtca cttttaatta accaaatcgt
     1381 cacaataatc cgccacgatg gagccacttt tttagggagg ctgcatcatg caaacgatta
     1441 tctgcgtcga acaactgagc aaaaccttca accagcatca ggcgctgcac gcggttgatc
     1501 tgaatatcca tcatggtgaa atggtggctc tgcttgggcc gtccggttcc ggcaaatcca
     1561 cccttttacg tcacttaagc ggtttgatta ccggcgataa atccgccggt agccatatcg
     1621 agctgctggg ccgcactgtc cagcgcgaag gccgcctggc ccgcgatatc cgcaaaagcc
     1681 gcgccaacac cggctacatc ttccaacaat tcaacctggt gaatcgcctg agcgtactgg
     1741 agaacgtgct gattggcgcg ctcggcagca cgccgttctg gcgcacctgt tttagttggt
     1801 tcacccgcga gcagaaacag cgcgcattac aggcgctgac ccgcgtcggt atggcgcact
     1861 ttgcgtatca gcgtgtttcc acgctctccg gcggccagca gcagcgtgtg gcgattgccc
     1921 gcgcgctgat gcagcaggcg aaagtgattc tcgctgacga accgatcgcc tcgctggacc
     1981 cggaatcggc gcgcatcgtg atggacaccc tgcgcgacat caaccagaac gacggcatca
     2041 ccgtggtcgt cacgctgcat caggtggatt acgccctgcg ctactgcgaa cgcatcgtcg
     2101 ccctgcgcca ggggcacgtc ttctacgacg gcagcagcca acagtttgat aacgaacgtt
     2161 ttgaccatct ctaccgcagc atcaaccgcg tcgaagagaa cgcgaaagct gcctgacatc
     2221 cccatcattg aggaaaacga atgaacgcta agataattgc ctcgctggcc ttcaccagca
     2281 tgttcagcct cagcaccctg ttaagcccgg cacacgccga agagcaggaa aaggcgctga
     2341 atttcggcat tatttcaacg gaatcacagc aaaacctgaa accgcaatgg acgccgtttt
     2401 tacaggatat ggagaagaag ctgggcgtga aggtcaacgc cttctttgcc ccggactatg
     2461 cgggcattat ccagggaatg cgtttcaaca aagtggatat cgcctggtac ggcaacctgt
     2521 cggcgatgga agcagtggat cgcgccaacg gccaagtctt cgctcagacc gtcgcggcgg
     2581 atggatcgcc gggttactgg agcgtgttga tcgtcaacaa agacagcccg atcaacaacc
     2641 tgaacgatct gctggcgaag cggaaagatc tcaccttcgg taatggcgat cctaactcca
     2701 cctctggctt cctcgtcccc ggctactacg tcttctccaa aaacaatatc tccgccagtg
     2761 acttcaagcg caccgtcaac gccgggcatg aaaccaacgc gctggccgtc gccaacaagc
     2821 aggtggatgt ggcgaccaac aacaccgaaa acctcgacaa gctgaaaacc tccgcgccgg
     2881 agaagctgaa agaactgaaa gtgatctgga agtcaccgct gatcccaggc gatccgatcg
     2941 tctggcgtaa aaacctttcc gaaaccacca aagacaagat ctacgacttc tttatgaatt
     3001 acggcaagac gccggaagag aaagcggtgc tggaacgcct gggctgggcg ccgttccgcg
     3061 cctccagcga cctgcaactg gtgccgattc gccagctcgc actgtttaaa gagatgcaaa
     3121 gcgtgaaagg caataaaggg ctgaatgagc aggacaagct ggcaaaaacc accgagattc
     3181 aagcgcagct ggatgacctg gaccgcctga acaacgcgtt aagcgcgatg agttcggtaa
     3241 gtaaagcgat gcagtaaatc gtaggtcgga taagacgccc cggtgtcgca tccgacaatg
     3301 tgcaggcgtt gatgccggat gcggtgcaag caccttatcc ggcctacaga ccggagccaa
     3361 acatgcaaac catcaccatc gccccaccca agcgcagctg gttctcgctt ctgagctggg
     3421 ccgttgtact cgccgtgttg gtcgtctcgt ggcagggcgc ggaaatggcc ccgctcacgc
     3481 tgatcaaaga cggcggcaac atggcgacct tcgccgccga cttcttcccg cccgatttca
     3541 gccagtggca ggattacctc accgaaatgc tcgtcacgct gcaaatcgcc gtctggggca
     3601 ccgcgctggc ggtggttctc tccatcccct tcggcctgat gagcgccgaa aacctggtgc
     3661 cgtggtgggt ttaccagccc gtgcgccgcc tgatggacgc ctgccgcgcc attaacgaaa
     3721 tggtcttcgc catgctgttc gtggtcgccg tcggcctcgg cccattcgct ggtgtgctgg
     3781 cgctgtttat ccacaccacc ggtgtgctct ccaaactgct ttccgaagcg gtggaagcga
     3841 ttgaacccgg cccggtggaa ggcattcgcg ccaccggcgc caacaagctc gaagagatcc
     3901 tctacggcgt gctgccgcag gtgatgccgc tgctgatctc ctactccctc tatcgcttcg
     3961 aatccaacgt ccgctcggcg accgtcgtcg gcatggtcgg cgcgggcggg atcggcgtca
     4021 cgctgtggga agcgattcgc ggtttccagt tccagcaaac ctgcgccctg atggtgctga
     4081 ttatcgtcac ggtcagcctg ctggatttcc tctctcaacg gttgcgtaag cactttatct
     4141 gattagcgag gcattgatag ctatgcactt gtctacacat ccgaccagct acccaacacg
     4201 ctatcaggag atagccgcaa aacttgagca ggagcttcgt caacactacc gctgcggcga
     4261 ctatcttccc gccgagcagc agctggcggc gcgctttgag gtaaatcgcc acaccctgcg
     4321 ccgcgccatc gaccaactgg tggaaaaagg ctgggtacag cgccgtcagg gcgtcggcgt
     4381 gctggtgctg atgcgcccgt tcgattaccc gctcaacgcc caggcgcgtt ttagccagaa
     4441 tctgctggat cagggcagcc atcccaccag cgaaaaactg ctgtctgtgc tgcgcccggc
     4501 ctccggtcac gtcgctgacg cgctggggat aaccgagggg gagaacgtca tccatctgcg
     4561 caccctgcgc cgggtcaacg gcatcgcgct ctgtttaatc gaccactact tcgcggacct
     4621 caccctctgg ccgacgctgc aacgcttcga cagcggctcg ctgcacgatt ttctgcgcga
     4681 gcaaacgggg attgcactgc gccgcagcca gacgcggatc agcgcccgcc gcgcccaggc
     4741 caaagagtgc cagcgtcttg aaattccgaa tatgtccccg ctgctgtgcg tgcgcaccct
     4801 taaccaccgt gacggtgaaa gcagcccggc ggagtactcc gtcagcctga cgcgcgccga
     4861 catgattgaa ttcactatgg agcactgaat gcacgcagat accgcgaccc gccagcactg
     4921 gatgtccgta ctggcacaca gccaaccggc agaactggca gcccgcctga acgcgctgaa
     4981 catcaccgcc aactatgagg tgatccgcgc cgctgaaact ggcctggtac agattcaggc
     5041 gcggatgggc ggcaccggcg aacgcttttt tgccggcgac gccacgctga cccgcgccgc
     5101 cgtacgtctg actgacggca ccctcggcta cagctgggtg ctggggcgcg acaaacagca
     5161 cgccgaacgc tgcgcgctga ttgacgcgct gatgcagcaa acccgccatt ttcaaaactt
     5221 atcagaaacg cttattgccc cgctggacgc tgaccgtatg gcacgcattg ccgcacgcca
     5281 ggccgaagtg aacgccagcc gggtcgactt ctttacgatg gttcgcggag acaacgcatg
     5341 accctggaaa ccgcttttat gcttcccgtg caggatgccc agcacagttt tcgtcgcctg
     5401 ttaaaggcca tgagcgagcc gggcgtgatt gtcgccctgc atcagctcaa acgcggctgg
     5461 cagccgctga atatcgccac caccagcgtg ctgctgacgc tggccgataa cgacacgccg
     5521 gtgtggcttg ccgcgccatt gagcaacgat atcgtcagcc agagcctgcg ttttcatacc
     5581 aacgcgccgc tggtcagcca gccggaacag gcgaccttcg cggtaacgga tgaggcgatt
     5641 tccagcgaac agcttaacgc cctttccacc ggcaccgccg ttgcgccgga agcgggcgca
     5701 acgctgattt tacaggtcgc cagcctgagc ggcgggcgca tgctgcgtct caccggcgcg
     5761 ggtattgccg aagaacgaat gatcgccccg cagctgccgg agtgcattct gcacgaactc
     5821 accgagcgcc cgcacccgtt cccgctcggc atcgacctga tcctgacctg cggcgagcgc
     5881 ctgctggctg ttccgcgaac cactcatgtg gaggtgtgct gatgtacgtt gccgtgaaag
     5941 ggggcgagaa ggcgatcgac gccgcccacg ccctgcaaga gagccgacgc cggggcgata
     6001 ccgatttgcc tgaactgagc gtcgcccaga ttgaacagca gcttaatctt gcggtggatc
     6061 gcgtgatgac cgaaggcggc attgccgacc gcgagctggc ggcgctggcg ctgaaacagg
     6121 ccagcggcga taacgtcgaa gcaattttcc tgctgcgcgc ctaccgcacc acgctggcga
     6181 agctcgcggt aagcgagccg ctcgacacca cggagatgcg tctcgaacgg cgtatttcag
     6241 cggtttataa agatattccc ggcggccagc tgcttggccc aacctacgac tacacccatc
     6301 gcctgctcga tttcaccctg ctggcaaacg gcgaagcgcc gacgctgacc accgccgaca
     6361 gcgaacagca gccgtcgccg cacgttttca gcctgctggc gcgtcagggg ctggcgaagt
     6421 ttgaagagga tagcggcgca cagccggatg acatcacccg cacgccaccg gtttacccct
     6481 gctcacgttc ttcccgcctg caacagctca tgcgcggcga cgaaggctat ttgctggcgc
     6541 tggcctactc cacccaacgc ggttacgggc gcaatcaccc gttcgcgggt gaaatccgca
     6601 gtggttacat cgacgtgtcg attgtgcccg aagagctggg atttgcggtg aacgtcggcg
     6661 aactgctgat gactgagtgt gaaatggtca acggttttat cgacccaccg gatgaatctc
     6721 cgcacttcac gcgcggctac gggctggtgt tcggcatgag cgagcgcaaa gcgatggcga
     6781 tggcgctggt cgaccgcgct ctgcaagccc cggagtacgg cgagcacgcg acaggcccgg
     6841 cgcaggatga agagttcgtg ctggcacatg ccgacaacgt cgaagccgca ggctttgtct
     6901 cgcacctcaa actcccgcac tacgtcgatt tccaggccga actggagcta ctcaaacgtc
     6961 tgcaacagga gcagaaccat ggctaatctg agcggctaca actttgccta cctcgacgag
     7021 cagaccaaac gcatgatccg ccgcgccatc ttaaaagcgg tggcgatccc cggttatcag
     7081 gtgccgtttg gcgggcgcga gatgccaatg ccgtacggct ggggaaccgg cggcattcag
     7141 cttaccgcca gcgtgattgg cgaaagcgac gtgctgaagg tgattgacca gggcgcggat
     7201 gacaccacca acgccgtgtc gattcgcaac ttctttaagc gcgtgaccgg ggtaaacacc
     7261 accgagcgca cggacgatgc aacggtcatc cagacgcgtc accgcatccc cgaaacgccg
     7321 ctgaccgaag atcagatcat tattttccag gtgccgatcc ccgaaccgct gcgctttatc
     7381 gagccgcgcg aaacggaaac ccgcaccatg cacgcgctgg aggaatacgg cgtgatgcag
     7441 gtgaagctgt atgaagatat cgcccgcttc ggtcatatcg ccaccaccta cgcctatccg
     7501 gtgaaggtga acgggcgcta cgtaatggac ccgtcgccga tcccgaaatt cgataacccg
     7561 aaaatggaca tgatgcccgc cctgcaactc ttcggcgccg ggcgtgagaa gcgcatctat
     7621 gcggtgccgc cgtttacccg cgtggaaagc ctcgatttcg acgatcaccc gttcaccgtt
     7681 cagcagtggg atgagccatg cgccatctgc ggatcgaccc acagctatct tgatgaagtg
     7741 gtgctggatg acgccggaaa ccgcatgttt gtctgctccg ataccgatta ttgccgccaa
     7801 cagagcgagg caaaaagcca atgaatcaac cgttactttc ggtcaataac ctgacccacc
     7861 tttacgcgcc gggcaaaggc tttagcgatg tctcttttga tttatggccg ggggaagtgc
     7921 tgggcattgt cggggaatcc ggctccggga agaccacgct gctgaaatca atttccgcgc
     7981 gtctgacgcc gcagcagggg gagattcact atgaaaaccg ttccctctat ggcatgagcg
     8041 aggccgaccg ccgtcgcctg ctgcgcaccg aatggggcgt agtgcatcag catccgctcg
     8101 acggcctgcg ccgccaggtc tcggcaggcg gcaatatcgg cgagcggctg atggcgaccg
     8161 gggcgcgtca ttacggcgat attcgcgcca ccgcgcagaa gtggctggaa gaggtggaaa
     8221 ttcccgccaa ccggatcgac gacctgccga ccaccttttc cggcggtatg cagcagcgtt
     8281 tgcagattgc ccgcaacctg gtgacgcatc cgaagctggt gtttatggat gaaccgaccg
     8341 gcgggctgga tgtgtcggtg caggcccgcc tgctcgacct gctgcgcggc ctggtggtgg
     8401 agctgaacct cgcggtagtg attgtcaccc acgatttagg cgtcgcccgc ctgctggcgg
     8461 accgtttgct ggtgatgaag caggggcaag tggtggagag tgggttaacc gaccgcgtgc
     8521 tcgacgaccc gcatcatccg tatacacagc tgctggtgtc atcggttttg cagaattgag
     8581 ccggtgccgg atgcggcgta aacgccttat ccggcctaca aatgcgctcc ccgtaggtcg
     8641 gataagacgc atcagcgtcg catccgacac ccgaaccacg aggcgaaaaa tgattaacgt
     8701 acaaaacgtc agtaaaacct tcatcctgca ccagcaaaac ggcgtgcgct tgcccgtcct
     8761 caaccgcgcc tcgctcaccg tcaacgcggg cgaatgcgtg gtgctccacg gccactccgg
     8821 cagcggcaaa tcaaccctgc tgcgctcgct gtacgccaac tatctgcccg atgaagggca
     8881 aatccagatc aaacacggtg acgagtgggt agacctggtc actgcgccag cgcgcaaagt
     8941 ggtggaaatc cgcaaaacca ccgttggctg ggtgagccag tttctgcgcg ttatcccgcg
     9001 catttcggcg ctggaagtag taatgcagcc gctgctcgat accggcgtac cgcgtgaagc
     9061 ctgcgccgct aaagccgcgc gtctgctcac ccgtctgaac gtgccggaac gcctgtggcg
     9121 ccttgcgcca tcgaccttct ccggcggcga acagcagcgc gtcaacatcg cccgtggctt
     9181 tatcgtcgac taccccattc tgctgctcga cgaacctacc gcctcgctgg acgccaaaaa
     9241 cagcgccgcc gtagtcgaac tgatccgaga agccaaagcc cgcggcgcag ccatcgtcgg
     9301 cattttccat gacgaagccg tacgtaatga cgtcgccgac cgcctgcacc caatgggagc
     9361 ctcttcatga ttatcaataa cgtcaaactg gtgctggaaa acgaagtggt aaacggttcg
     9421 ctggagatgc aggacggcga aatccgcgcc tttgccgaaa gccagagccg cctgccggag
     9481 gcgatggacg gcgaaggcgg ctggctgctg ccggggctga ttgagctgca tacagacaac
     9541 ctcgataaat tcttcacccc gcgcccgaaa gttgactggc ccgcccactc ggcgatgagc
     9601 agccatgacg cgctgatggt ggcgagcggc atcaccaccg tactggacgc cgtggcgatt
     9661 ggcgacgtgc gagacggcgg cgatcggctg gagaatctgg agaagatgat caacgccatc
     9721 gaagagacgc agaaacgcgg cgtcaaccgc gccgagcacc gcctgcacct gcgctgcgag
     9781 ctgccgcata acaccacgct gccgctgttt gaaaagctgg tgcagcgcga gccggtgacg
     9841 ctggtgtcgc tgatggatca ctcgccgggc cagcgccagt tcgccaaccg cgagaagtat
     9901 cgcgaatatt atcagggcaa atactcgctc accgatgcgc agatgcagca gtatgaagag
     9961 gaacagctgg cgcttgccgc acgctggtcg cagccgaatc gcgaatccat cgccgcaatg
    10021 tgccgcgcgc gacaaattgc gctcgccagc cacgatgacg caacgcacgc ccacgttgcc
    10081 gaatctcacc agcttggcag cgtgatcgcc gaatttccca ccacgtttga agcagcggaa
    10141 gcctcgcgca agcatggcat gaacgtgctg atgggcgcgc caaatatcgt gcgtggcggt
    10201 tcgcactccg gcaacgtggc ggccagtgaa ctggcacagc ttggcctgct ggatatcctc
    10261 tcttccgact actaccccgc cagcctgctg gatgcggcgt tccgcgtcgc cgatgaccag
    10321 agcaaccgct ttacgctgcc gcaggcggtg aagctggtga ccaaaaatcc ggcgcaggcg
    10381 cttaatctcc aggatcgcgg ggtgattggc gagggcaaac gcgccgacct ggtgctggcg
    10441 catcgcaagg gcaatcacat tcatatcgac cacgtctggc gtcagggtaa aagggtgttc
    10501 tgatgatggg aaaactgatt tggttaatgg ggccgtccgg ctccgggaaa gatagcctgc
    10561 tggcggaact ccgcctgcgg gaacaaactc agctactggt ggcgcatcgc tacatcacgc
    10621 gcgacgccag cgccggaagc gaaaaccata tcgccctgag cgagcaggaa ttttttaccc
    10681 gcgcggggca aaatctgctg gccttaagct ggcacgctaa cggtctgtat tatggcgtcg
    10741 gcgtcgagat tgacctctgg ctgcacgctg gattcgacgt gctggtcaac ggctcacgcg
    10801 cccatctgcc gcaggcgcgg gcgcgctatc aatcggcgct gctgcccatt tgcttacagg
    10861 tttcgccaga gatcctgcgc cagcgcctgg aaaaccgtgg ccgcgaaaac gccagtgaaa
    10921 ttaacgcccg gctggcgcgc gccgcccgct atactccaca ggattgccat acgctcaata
    10981 atgacggcag cctgcgccag tcggtcgaca cgctgctgac gctaatccat cataaggaga
    11041 aacaccatgc ctgcttgtga gctttcgccc ggccacgcag tacgacaccg acgcggttta
    11101 cgcgttgatt tgcgagctaa aacaggcgga gtttgaccac cacgcgtttc gcgtgggctt
    11161 taacgccaac ctgcgcgacc cgaacatgcg ctaccatctg gcgctgctgg atggcgaagt
    11221 tgtcggtatg atcggcctgc atttgcagtt tcatctgcat catgtcaact ggatcggcga
    11281 aattcaggag ttggtggtaa tgccgcaggc gcgcggtctg aacgtcggca gtaagttact
    11341 ggcgtgggca gaagaagaag cccgccaggc cggggccgaa atgaccgaac tttcgaccaa
    11401 cgtgaagcgc cacgacgcgc accgtttcta tctgcgcgaa ggctacgagc agagccactt
    11461 ccgcttcacc aaggtgctgt aacatgagcc tgaccctcac gctcaccggc tccggcggcg
    11521 cacagggcgt tccggcatgg ggctgcgagt gtgcggcctg cgccagagcg cggcgctcgc
    11581 cgcactatcg ccgccagcca tgcagcggcg tagtgaagtt taacgaggcg atcaccctga
    11641 tcgacgccgg gcggcacgat ctcgccgatc gctggtcgcc cggatcgttc cagcagtttt
    11701 tgctgacgca ctaccatatg gatcacgtgc aggggctatt cccgctgcac tggggcgtgg
    11761 gcgatccgat cccggtttac ggcccaccgg atgaacaggg ctgcgacgat ctgtttaaac
    11821 atccgggcct gcttgatttc agccacacgg tggaaccgtt tgtggtgttt gatttgcagg
    11881 ggttacaggt cacacccctg ccgctcaacc actcaaaact gaccttcggt tatctgctgg
    11941 aaacggcgca cagccgggtg gcgtggctgt ctgacaccgc tggcttgccg gaaaaaacgc
    12001 tgaaattttt acgcaataat cagccgcagg taatggtgat tgattgcagc cacccgccgc
    12061 gcgaggatgc accgcgtaat cactgtgatt taaataccgt gcttgcgctg aatcaggtta
    12121 tccgctcgcc gcgcgtggtt ctgacccata tcagccacca gtttgatgcg tggctgatgg
    12181 aaaacgcact gccgtcgggg tttgaggcgg ggtttgatgg gatggagatt ggggtggcgt
    12241 gatgagaggg aatgtgcgtg ctggccccct caccctaacc ctctccccag aggggcgatg
    12301 cctgccccat acccaacatc attgcccata ctcatcttcc attctccgct cttcatcttc
    12361 tagctgccga cgctcctgat taagctggtg ctggcgatcg tccagctgcc tgcgacgatc
    12421 ttcaaactgg cgacggcggt cgtcatattg tctgcgccga tcgtcgctca cttcacgctg
    12481 ccagccgtcg tcgcgcgaat cttcataatc tcgcccacgg tcagggttag aagcgtcgtt
    12541 aatcgcctgc tgaatattgc caatggtgtc atcgataata tcggcctggg ccggtacggt
    12601 ggtcagcgtg agcagggtga aaagaaaaag cgtggagtgg cgtttcataa actcggcctc
    12661 gcaacaaagt taatacaagg taggcaaagg tgcctgttac gagtagagga ggattctcaa
    12721 ttctcttgcc ccagcgcctg cgccaactgc gcacgggtaa agggtttgcg caacagcgcc
    12781 acgtctggca gcgcggggtt atggctgggg cgtaaatcct gaccgctgat gagtagcagc
    12841 gtcaggtgcg ggtaaagttt gcgggccgca ttgaccactt ctgcgccgct catgccgccg
    12901 gggagcatta aatcgctgat aaaaatatcg atttctgctg atgccgccag cagatccagc
    12961 gcctgttcgc cgctgcttgc ttccagcgtc aaatagccca gcaggtgcaa ctgttcgcag
    13021 atggtctggc gcaccgcggc ttcatcttcc agcactaaca ccagtttatc gccgctgaca
    13081 gaagcctgtt ctactgccgc tggcgatagg tttttcgccg ccgtcagcgc gcggggaagc
    13141 tgtaatctga ccgttgtccc ctgccccggc gcgctttcaa tttcaacgcg cccaccggac
    13201 tggcgcacaa aaccatagac catcgacaaa cccagcccgc tgccgctgcc cgtctgtttg
    13261 gtggtaaaga atggctcaaa tacctggctt ttcaccgcct gcgacatacc gcagccatga
    13321 tcgatgacct ccagtgccac catgtcctga cgctgcccac tgctgcgagt aacgcgctga
    13381 ttccaggtac gaattttgat gattcccgtc tgcccttcca tcgcgtcacg ggcgttcatc
    13441 accaggttga tgatggcatt ttccagttgg ctgatgtcaa tccacgcggg ccacgccgga
    13501 gactgcgctt caatctccag cgccagcgtt gccggtaagg agtggcgcat taattcgctt
    13561 aactcttcca gcaatgtttt cagttcaacg gcttgcggat gtaaggactg cttgcgggaa
    13621 aacgccagca gtcgttgtgt cagtagcgcc ccacgttcgg cagcttgcag cgcccgctgt
    13681 attcgcggcg cgtctggcga gtccggattc accagctcca ggctgccaat aatcaccgcc
    13741 agcagattat taaaatcatg ggccagcccg cccgtcagtt gcccgacagc tttcagtttc
    13801 tggctgtgta atagcgcctc ttccaacccc tgccgctcag tttgggcaat ctccatctgc
    13861 gtggtcttct cttttagcag ccgtgaggtt cgttcgagtg acgcggtatt gcgggcaaaa
    13921 acgttaaagg cgcgcgccag ttcacccaac tcatcacgcc gctgtaacgc cggaaaagtg
    13981 acgtccgctt cgccgtgcgc caggcggctc atggcgcggg aaatcgccgt caggttagtg
    14041 ccgaggttgc gatagatata ccagccggca aacgcggtga tcaccaccgc taacagggcg
    14101 aaaatcatga tcaccctctc gccggagcgc aaatcctggt aactttgcgc cacacgctga
    14161 ccggagagcg tcgccacctg ttcggcgtaa cggttgatgt cgccattgag gatcgccacc
    14221 agcgctttga tgtgaaacat ataccagctg attgccagat cgctttgctc cagagcggca
    14281 gaaagcggcg cgagtttacg catttcatca ttgaaatcat tgaaatcatt gagtaacgta
    14341 ttaaccaatg gcgtatcgct gtgggtcggc aacgcagaca ttaccccgac cagttgatga
    14401 ataatcgcct gcggcgcggg ggtggcaata gcggcaacaa tcagtcggtt catctcgtta
    14461 agcaaaatat catcttgcgc gtgagtgact ttttgcagat gccgcagata acttaagttt
    14521 tgatacagcg aacttaacag cgcgttgcgc tccagatgcc gcctttgccc gcgctccagc
    14581 ataccgccga cgctggtttg cagttcgttg ctacgcgtga tgattcgcgt caccagcccg
    14641 gcttcctgct gcgctaaagg cgcgttcgcc agttgtgcaa gcgagctttt taatgcccgc
    14701 tgtgtctcct gcaaccgctc ggcttcgcct ttatattcca gcgctcccac cacctgcgac
    14761 aatcgcaccg ccgccgttgc cacgtttgcc gtgtcgcgcg ccagattcag gctgccggtc
    14821 atatcatcca ccgtttgctg ctgcaccagc tcctgcatct ggctggcatg acgaaagccc
    14881 attaccgcca cgccgctgac catcaacgtc accgccacca ccagcaaatt caaaagcagc
    14941 aatcgcccgc gcgcgctggc aaaaaagtga cgtccggggt gcgccatatc acgctccgtg
    15001 aagaaaaatc gcaatatgac aaatatgaac ggcttctgac attttgcatt tactttcctg
    15061 tgatggagtg ctgatatcga caacctcgca ggagtccacc atgaacagca cgccagttct
    15121 ggagatgcgc aatattgcca aagccttcgg caaattttat gcactcaaag gggtggattt
    15181 gacggtctac cctggcgaga tccatgccct gatgggggaa aacggcgcgg gaaaaagcac
    15241 gctgatgaag gtgctggcgg gggcgtatac cgccaccagc ggcgagattc tcatagacgg
    15301 caagcccttt cacattcgca cgccaaaaga tgccttaagc gccggtatta cgctgattta
    15361 tcaggagatg cagctggcac cgaatctgtc ggtggcagaa aatatttttc tcggcagcga
    15421 gctttcccac ggcgggctgg tgcagcgtaa agagatgcta gtgcaggcgc aaaaagtgat
    15481 cgaccgcctc ggcgcgcagt ttaacgccag cgataaggtc atgacgctga ccattgccga
    15541 gcaacagcag gtcgaaatcg cccgcgcact acatcgcaac agccgcattc tggtgatgga
    15601 cgaacccacc gctgccctct cctcccgcga aactcaccgc ctgtttgaac tgattatgcg
    15661 gctgcgcgat gaagggatgg cgattatcta cattagccac cgcatggcgg aagtgtatga
    15721 gctttccgat cgcgtcagcg tgctacgcga cgggcaatac gttggcagcc tgacccgcga
    15781 taacctcaat gccggggagc tggtgcggat gatggtcggc aggccactga gcgatctgtt
    15841 caataaagag cgcgatatcc cgctcggtaa agcccgcctg aatgttcacc acctgaccga
    15901 cggcggcaaa gtccagccga gtagcctgct ggtgcgttcc ggcgaaattg ttggcctcgc
    15961 cggactggtg ggtgccggac gttccgaact ggcgcagttg atcttcggcg tgcggaaagc
    16021 gacaggcgga atgattgaag tcgatggtga accggtggtg atccactccc cgcgcgaagc
    16081 catcgatctt ggcattggtt ttctcaccga aaaccgcaaa gaacaaggct tattccttga
    16141 aatggcagca gccgaaaaca tcaccatggc aaccctggag cgcgatgccc gctggggaat
    16201 gctcaatcgc aaaaaagcgc aaaccatttc cgatgacgcc attaagttgc tcaacattcg
    16261 cgtgcctcat gcccaggtac gcgcgggcgg gctttccggc ggcaatcagc aaaaactgtt
    16321 gatctcccgc tgggtggcga ttggcccgcg cattttactg ctcgatgaac ccgcccgcgg
    16381 cgtggacgtt ggcgccaaaa gtgagatcta ccggatcatg aacgagatgg cgcgcaaggg
    16441 cgtggcgatc ctgatgatct ccagcgaact gccggagata gtgggaatga gcgatcgcgt
    16501 ctatgtgatg cgcgaaggca gcattgccgg tgagttaaac ggcaaaaaca tcacccagga
    16561 aaacattatg accttagcga ctggcgtcaa cgacgcccat tcccaggcgg taacctcatg
    16621 acgacctctg caaaaacctc tgccaaaaaa atgctgatga gcgatctgat gcaaacggtc
    16681 ggcatcttgc ccattttgat tctgatcgtg gcggtatttg gctttatcac gccaaacttt
    16741 tttaccgaaa gcaacctgct gaatattacc cgccaggcat cgatcaacat tgttctggcg
    16801 gcggggatga ccttcatcat tttgaccggc gggatcgatc tttccgtggg ttcgatcctc
    16861 ggcaccacag cggtggcggc gatggtcgtc tcccttagcc ctgagttcgc catgctgtcg
    16921 atcccggcag cgctgatgct cggcctgctg ctggggctgt tcaacggcgc gctggtggcc
    16981 tttgccggat tgccaccgtt tatcgttacg cttggcacct atacggcgct gcgcggggcg
    17041 gcgtatctgc tggcggacgg caccacggtt atcaactcca acattaattt cgagtggata
    17101 ggcaacaact atctcgggcc ggtcccatgg ctggtggtga ttgcgctggc ggtgatcgtg
    17161 gtgtgctggt ttattctgcg ccgcaccacg cttggcgttc atatctacgc cgtcggcggc
    17221 aatatgcagg cggcgcgtct taccggcatt aaggtctggc tggtgctgct gtttgtttat
    17281 ggcatgagcg gcctgctttc tgggcttggt gggattatga gcgcctcacg gctgtacagc
    17341 gccaacggca acttaggcat gggttacgaa ctggatgcca ttgcagcggt gatcctcggc
    17401 ggcaccagtt ttgtcggcgg catcggcacc atcaccggca cgctggtcgg cgcgctaatt
    17461 atcgccacgc tcaacaatgg catgacctta atgggcgtct cttacttctg gcaactggtg
    17521 atcaaagggg cgatgatcat cattgcggtg ctgatcgaca aataccgtac ccgacaccat
    17581 caaagtgcat aaacacatga ggaatacagc atgagattga agcccttagt gaccgcgctc
    17641 tgtgcaggcg cgctgctggc cgcagcaccg ttcgcccagg cgaaagaact gaaatcaata
    17701 ggcgtaacgg tgggcgatct ggcgaacccg ttcttcgtgc agatcaccaa aggcgcggag
    17761 ctggaagcac gcaaactggc aggcgataac gtcaaagtga cgctggtttc cagcggctac
    17821 gatctgggtc agcaggtgtc gcagatagac aactttatcg ccgctaacgt cgatatgatt
    17881 atcctcaacg ctgccgattc aaaagggatc ggcccggcgg tgaaacgggc gaaagatgcc
    17941 gggatcgtgg tcgtcgcggt tgacgtagcg gcagaaggtg ccgatgccac catcacttcc
    18001 gataacaccc aggcaggcga aatggcctgt aaatacatca ccgatcgcct gaaaggcaaa
    18061 ggcaacgtgg tgatcatcaa cggcccgccg gtatccgccg tgcaaaaccg cgtcgaagga
    18121 tgccagactg aatttaaacg ccatcctgac atcaaagtgc tctctgacaa ccagaacgcg
    18181 aaaggcagtc gtgaaggcgg gctggaggta atgacgggac tgctggcggc aaatccgaag
    18241 atcgacggcg tatttgcgat taacgatccc accgcaatcg gtgccgatct ggcagcaaaa
    18301 caggcacaac gtaacgagtt ctttattgtc ggtgtggacg gaagcccgga cgccgaagag
    18361 gcgctcaagc gtgagaactc gttgtttgtc gctactccgg cgcaagatcc ccaggtgatg
    18421 gcagcgaaag cggtggaaat tggctacgac attttgcagg gcaaacctgc gccgaaagag
    18481 ccggtgctga tcccggtgac gatgatcgat aaaaacaacg tcggcaatta caaaggctgg
    18541 acggtgaaat aagtccacac gccgggcggc gcagctgccc ggtcaccgct aaggagaacg
    18601 ctttcgatga aacgctccgc tataaacgac attctcggcc atacacggca attcttttcg
    18661 caacatgacg tacatctgcc gccctttgcc agtttctcgc ctgctcaatg gcagcagctc
    18721 gacaccgccg cgtgggagga ggtttttgat cttaaactcg gctgggatgt taccgctttt
    18781 ggtcgcaaca actttgcagc acacgggctg acgctgttta ccctacgtaa cggctcagca
    18841 aaaggaatgc cgtatgtgaa atgttacgcc gagaagatca tgcatgtgcg cgatgcgcag
    18901 gtcacgccaa tgcattttca ctggcgtaag cgtgaggaca ttatcaatcg cggcggcggt
    18961 aatttaattg ttgaactgtg gaatgccgat agtaacgagc aaacggctga tagtgacatt
    19021 acggtggtga tagacggctg ccgccagaaa catactgcag gctctcagtt gcgcctgtct
    19081 cccggcgaaa gtatctgcct gccgcccggc ctgtatcaca gcttttgggc agaagccggt
    19141 tttggcgatg tgctggttgg cgaagtctct tctgttaatg acgacgacca cgataaccac
    19201 tttttacagc cactggaccg ctacaacctg attgacgaag acgaacctgc acagttggta
    19261 ttgtgtaacg aataccgcca gttccgctga ataaggataa cgttatgcca ttgatttctc
    19321 tcgctgaagg gctggcacat gcccgcgaac atcactattc gttaggtgcg tttaatgtgc
    19381 tggactcgca ctttttacgc gccttgtttg ctgctgccag acaagaacgt tcgcccttta
    19441 ttatcaacat tgccgaagta cattttaaat atatctcgct ggattccctg gtcgagacag
    19501 tgaaatttga agcggcgcgt catgacattc cggtggtgtt aaatctcgat cacggactgc
    19561 attttgaatc ggtggtgcaa gcgttgcggc tgggattcag ctcggtaatg tttgatggct
    19621 ccacgctggg gtatgaagaa aatgtccgcc agacgcggga agtggtgaaa atgtgccatg
    19681 ccgttggcgt atcggtagaa gctgaactgg gcgcggtggg tggcgacgaa ggcggtgcac
    19741 tctatggtca tgccgatgaa tccctgttta ccgatccgca actggcgcgt gattttgtcg
    19801 accgtaccgg cattgacgcg ctggccgtgg cgattggcaa tgcacacggc aaatatcgcg
    19861 gcgaaccgaa actcgatttc ccgcgcctcg acgctatccg ccagcaggct gctatcccgc
    19921 tggtattaca cggcggctca gggattagcg atgccgattt ccgccgcgcc atcgaactgg
    19981 gtatccacaa aatcaatttc tacactggca tgtcgcaagc ggcgctggct gccgttgagg
    20041 cccgaatggc gcaccgtcag ccgatgtatg acgagtttgc cgagttactg ctggcagtgg
    20101 aagccgccat cagcgatacg gttgcacaac agatgcgtat ttttggcagt gcggggcagg
    20161 catgatggag cgtaaaggga ttatcgccgc aggcaatatg ttggttgacc acgtccatca
    20221 gattatccag tggccagagc gcggctggct ggcggagatc acccacagcg aacgctccac
    20281 cggcggtgcg ccgcttaacg tcctgctgac gctggcgaaa atgcgcaccg ggctgccgtt
    20341 acaggcggtt gggctaatcg gtcaggacag cgacggcgat tacatcatgg cgatgcttga
    20401 gcaatatcac gtcaatcgtc agcatgtgca gcgcactacc tttgccccga cgtcgatgtc
    20461 gcaggtgatg acagacccaa gcgggcagcg gacgtttttc cattcgccgg gtgcaaacag
    20521 acttttggat cttccggcgt tcgatcagct cgataacacc atgaagattt tccatctcgg
    20581 ctatttactg ttgctcgaca gcctggatat gccggacgat gtttacggca cccgtagcgc
    20641 acgcctgctg gcgcaaatgc gtgatactgg ctttgaaacc tcactcgacc tggtctcccg
    20701 taagggcgat ccgcgttatc agccgctggt gctgcccgcg ttgcgccatc tggattatct
    20761 ggtaattaac gaactggagg ccggggagtt tagcgggctg gaaattcggt tacccaacgg
    20821 cgaaccgcat atcgcgcata ttgctgccgc cgcgcgtgaa ctgttagatg cgggcgtgcg
    20881 ccagcgggtg gtgatccact acccggaagg ggcgtgggga gagaccccag aacagggcgg
    20941 cgtgtggatc ccttcgcaaa agttagaaca acgggaaatt atcggcagcg tcggcgcggg
    21001 cgatgcattt tgtgccggat ttttatatgg ctgccatgaa cgctggacgc ttaccgagag
    21061 catcaaactg gcacacgcct gcgcgcgggc aagcctgtta tgcgccaatg ccatcgacgg
    21121 ggcgaaaacg ctggaagagc tgcaaacaga aatgtcggat taatgcacgt cggcagcgaa
    21181 gacatacccc agaccgcgca gagttttaat caacatcggc tgatgcgggt tgagttcgat
    21241 tttgcgtcgt aagcgcatga ttaatacgtc gatggtgcga tcaaacacct ccacgctgtc
    21301 gctgtgggtg aacgccagca actgctcgcg gctcaatacc cgacgcgcgt tttgcgccag
    21361 agccagtaac aagccatatt cgccctgagt taactcgaca tgttgctgaa gcggattgtg
    21421 caactggcag cgcatggtat cgagatacca gccattgaat gacagcccgg cgcttttctc
    21481 gtcattaacc gccagcgcgc caacccgtcg caacaccgct ttcagtcgtg cgaccaccac
    21541 ccgcggggtg aacggtttgc agatgtaatc atccgcgccc atttccagcc cgaccaccac
    21601 atcagactca ctgcccatgc cggtcaacat caccaccggc aatgtcggac gcagtttctg
    21661 gatttgctgc aataccctca ggccgttggt atcgggtagc atcatatcca gcaccaccag
    21721 cgcaatgtca ggatcgccct caatacgcaa tatggcctcc tgtccggtat ggcagactga
    21781 aaccgtaaag acatgttcac ttaacacgtc ctgcaacagt gcgcagatag ccgtgtcatc
    21841 atccaccacc agcacgacgg gtttcatggt tacactccgc atcattcatc tgattaatca
    21901 ttgtgcgtac ttcttgtcga agtgaaagct gaaagtaata taaataataa aaatataagt
    21961 caggtcacat tattgatgat agtaataaat atgtcgtact tttccctctt cagcacaaat
    22021 gcctaataaa atggatcgtt ccgctcacac ttccacttat taaagtcgca atttcccaat
    22081 tatgataacg ccagctttaa atgacgtatt ttgcatggat tacatattta cgacataaat
    22141 gataaatgga gatttatatg aataactctc ggttattccg tttgagcagg attgttattg
    22201 cgttaactgc cgccagcggc atgatggtaa ataccgctaa cgcgaaagag gaagcgaaag
    22261 ccgccactca atatacccaa caggttaatc agaattacgc caaatcatta ccgtttagcg
    22321 atcgtcagga ttttgacgat gcccagcgtg gatttatcgc cccgctgctg gatgaaggta
    22381 ttctgcgtga tgcgaacggt aaagtttact accgcgcaga cgattacaaa tttgatatta
    22441 atgccgctgc gccggaaacc gtaaacccca gcctgtggcg tcagtcgcag atcaacggca
    22501 tttctggcct gttcaaagtc accgataaaa tgtatcaggt gcgcggccag gatatctcta
    22561 acattacgtt cgttgagggg gagaaaggca ttattgtcat cgacccgctg gtgacgccgc
    22621 ctgccgcaaa agccgcactt gacctttact tccagcatcg tccgcaaaaa ccgattgttg
    22681 ccgttatcta cactcacagc cacaccgacc actatggtgg cgtgaaaggc attatctctg
    22741 aagccgatgt taaatccggc aaagttcagg tgattgcccc tgctggcttt atggacgaag
    22801 ccatcagcga aaacgtgctg gcgggtaaca tcatgagccg ccgtgcgctc tactcttacg
    22861 gtctgttact gccgcacaac gcgcaaggca acgtgggtaa tggccttggc gtgacgctgg
    22921 caacgggcga cccgagcatt attgcaccga ctaaaactat cgtcagaact ggcgagaaga
    22981 tgattatcga cggcctggag tttgacttcc tgatgacccc aggtagcgaa gcgccagccg
    23041 aaatgcactt ctatattccg gccctgaaag ccctgtgtac cgccgagaac gccacgcata
    23101 ccctgcacaa cttctacact ctgcgcggcg cgaaaacccg cgataccagc aagtggaccg
    23161 agtacctgaa cgaaacgctg gatatgtggg gtaacgacgc ggaagtactg tttatgccgc
    23221 acacatggcc ggtctggggc aataagcata tcaatgatta tattggtaaa tatcgcgata
    23281 ctatcacgtt cattcacgac cagaccctgc acctggcgaa ccagggctac accatgaatg
    23341 aaatcggcga catgattaag ctgccgcctg cacttgccaa taactgggcc agccgtggct
    23401 attacggttc tgtcagccac aacgcccgcg cggtgtataa cttctatctt ggctattacg
    23461 acggtaaccc ggctaacctg catccgtatg gtcaggtaga gatgggcaaa cgttacgtga
    23521 aagcgctggg cggctctgcc cgtgtcatca acctggcgca agaagcgaat aagcaaggtg
    23581 attaccgctg gtcggcagaa ctactgaaac aggtgattgc cgccaacccg ggtgaccagg
    23641 tggcgaagaa tctgcaagcg aataactttg aacagctggg ctatcaggcc gagtccgcca
    23701 catggcgcgg tttctacctg accggcgcga aagagctgcg cgaaggggtg cataagttca
    23761 gccacggcac caccggttcc ccggacacca ttcgcggaat gtcggtcgaa atgctgttcg
    23821 actttatggc cgttcgcctc gatagcgcga aagctgcggg taaaaatatc agcctgaact
    23881 tcaatatgag caacggcgat aacctcaacc tgacgctgaa cgatagcgtg cttaactacc
    23941 gtaaaacact gcaaccgcaa gccgacgcct ctttctacat cagccgtgaa gatctgcacg
    24001 ccgtgctgac cggacaggca aaaatggcgg atctggtgaa agcgaagaaa gccaaaatta
    24061 ttggcaatgg cgcgaaactg gaagaaatta tcgcctgtct ggataatttc gatttgtggg
    24121 tgaatatcgt aaccccaaat taatgtcact cgcttttggg ggcgctgaag cccccaaaag
    24181 catttatttt aaaaatatat acagccaatt aataaataaa atatataagt tatgaatgcg
    24241 gtggctgaat aaagctattt ttattcatca agaaaatagc catactgacg cattcaatgg
    24301 atattgatat tcactacaga tattgttcgc agcagacagg atgtatttaa tatgccaaca
    24361 gttctctctc gcatggcgat gcaactcaaa aaaacagcct ggataattcc cgtcttcatg
    24421 gtttcgggat gctcattatc tccggcaatc ccggtgatcg gcgcttatta tcccagctgg
    24481 tttttctgcg ccattgccag ccttatattg acgctcatca cgaggcgagt tattcagcgg
    24541 gcaaatatca agctggcatt tgtcggaatt atttataccg ccctttttgc tctctacgcc
    24601 atgctgttct ggctggcatt tttctaatct atttgagacg cgctcatgga aagtacgccg
    24661 aaaaaagctc ctcgcagtaa attccctgct ctgttagtgg ttgcgttggc gctggttgcc
    24721 cttgttttcg ttatctggcg cgtagacagt gcgccatcaa ctaatgacgc ttacgtgtca
    24781 gcagatacca ttgatgtggt gccggaagtc agcggccgca ttgtagaact ggcggtcacc
    24841 gacaaccagg cagtcaaaca gggcgatttg ctgttccgca ttgacccgcg cccgtacgaa
    24901 gccaatctgg cgaaatctga agcctccctc gcggcactgg ataagcaaat tatgctcacc
    24961 cagcgtagcg ttgacgcgca acagtttggt gccgactcgg ttaacgccac ggtagaaaaa
    25021 gcccgtgccg ccgctaaaca ggccacagat acattacgcc gcaccgagcc attactgaaa
    25081 gaaggttttg tctcagcgga agacgttgac cgtgcgagga ccgcgcagcg cgctgcagaa
    25141 gccgatctta atgctgtatt gttgcaggcg cagtcagccg ccagcgccgt cagcggcgtg
    25201 gatgcattag ttgcccagcg tgcggcggtc gaagcggata ttgccctgac caaactgcat
    25261 ctggaaatgg cgaccgttcg cgcaccgttt gatggccggg tcatttccct caaaacctcc
    25321 gtcgggcaat ttgcttctgc catgcgccct atttttaccc taatcgacac tcgtcactgg
    25381 tacgtgatcg ccaacttccg cgaaaccgat ctgaaaaata ttcgctcagg tacacctgca
    25441 acgattcgcc tgatgagtga cagcggcaaa actttcgagg gtaaagtgga ttcaattggc
    25501 tacggcgtgc taccggatga cggcggcctg gtgctgggcg gcctgccgaa agtttctcgt
    25561 tctattaact gggtccgtgt tgcccagcgt tttccggtca aaatcatggt tgataaacct
    25621 gacccggaaa tgttccgcat cggcgcttcg gcagtcgcta atcttgagcc gcaataatga
    25681 gcgcgctcaa ctccctgcca ttaccggtgg tcaggctgct ggcgttcttt catgaagagt
    25741 taagcgagcg gcgaccaggt cgcgtgccgc agaccgtgca actctgggta ggctgcctgc
    25801 tggtgattct gatctcgatg acttttgaga tcccttttgt ggcgttatcg ctggcagtgc
    25861 tgttttacgg tattcagtcg aacgcgtttt acaccaaatt tgtcgcgatc ttgtttgtgg
    25921 ttgccacggt gctggagatc ggcagcctgt ttttgatcta caaatggtca tacggcgaac
    25981 cgttgatccg cttgatcatc gccgggccga tcctgatggg ctgcatgttt ttgatgcgca
    26041 cccatcggtt gggactggtc tttttcgctg tcgccattgt cgccatttat gggcaaacct
    26101 tccccgccat gctcgactat ccggaagtgg tcgtgcgctt aacgctgtgg tgtatcgttg
    26161 ttggcctcta cccgacgtta ttaatgacgt taatcggcgt actgtggttt cccagtcgtg
    26221 ccatttcgca aatgcatcaa gcgcttaatg atcggcttga tgatgccatt agccacctga
    26281 cggacagcct cgcaccgcta cccgaaacgc ggattgaaag agaggcgctg gcgctgcaaa
    26341 aactcaatgt cttttgcctc gcggacgatg ccaactggcg aactcaaagc gcatggtggc
    26401 aaagctgcgt ggcaacggta acctacattt actcgacgct gaatcgctac gatcccacct
    26461 cttttgctga ttctcaggca attattgaat tccgacaaaa attagcttca gaaatcaaca
    26521 agctgcagca tgccattacc gaaggtcagt gctggcaaag cgactggcgg atcagtgaaa
    26581 gtgaagcgat ggcggcacgg gaatgtaacc tggagaatat ctgccagacg ttgttacaac
    26641 tgggtcagat ggacccgaat acgccgccaa cgcccgccgc caaaccgcca tcaatggtcg
    26701 ctgatgcttt taccaatcca gactatatgc gctacgcggt aaaaacgctg ctcgcctgtt
    26761 tgatctgtta caccttctac agcggcgtgg actgggaagg cattcacacc tgtatgctga
    26821 cctgcgtgat cgtcgctaat ccgaatgtcg gttcatcgta ccagaagatg gtgctgcgtt
    26881 ttggcggggc cttttgcggc gcgattctgg cgctgttatt cacgctactg gtcatgccct
    26941 ggctggacaa tattgtcgaa ttgctgtttg tgctggcacc gattttcctg ttgggcgcat
    27001 ggattgccac cagctctgaa cgctcttctt atatcggcac acagatggtg gtcaccttcg
    27061 cgctcgccac gctcgaaaac gtttttggtc cggtgtacga cctggtggaa attcgcgatc
    27121 gcgccctggg tatcatcatt ggtaccgtgg tgtctgcggt gatttacacc tttgtctggc
    27181 ctgaaagtga agcgcgcacg ctgccgcaaa aactggctgg cgcgctgggt atgctaagta
    27241 aagtaatgcg gatcccacgc cagcaggaag tcacggctct gcgcacttat ctgcaaattc
    27301 gtataggtct gcatgcggcg tttaatgcct gtgaagagat gtgccaacgc gtggcgctgg
    27361 agcgtcaact ggacagcgaa gaacgcgcct tactgattga acgttcgcaa acggttattc
    27421 atcagggccg cgatcttctt cacgcctggg atgccacctg gaactcggcg caggcgctgg
    27481 ataacgcact acagccggac aaagcaggtc agtttgccga cgccctggaa aaatacgctg
    27541 ccggtctggc aaccgcactc agccgttctc ctcaaataac gcttgaagag acacccgcct
    27601 cgcaggccat cctgcccacc ttattaaaac aggagcaaca cgtctgccag cttttcgccc
    27661 gcttgccaga ctggacagcc ccggcattaa cgcccgccac ggaacaggca caaggagcca
    27721 cgcaatgatc aatcgtcaac tttcacgtct gctgttgtgc agcattctcg gcagcacgac
    27781 gctgatttcc ggctgtgccc tggtacgcaa ggattctgcg cctcatcaac agctcaaacc
    27841 ggaacaaatc aaactggccg atgatattca tcttgccagc tccggctggc cgcaggcgca
    27901 gtggtggaaa caactcaatg acccgcagct ggatgcgctg atccaacgga cgctaagtgg
    27961 ttcacacacc ctcgccgaag cgaaactgcg ggaagaaaaa gcgcagtccc aggccgattt
    28021 gttagatgcc ggttcacaat tacaggtcgc agcgttaggg atgctcaacc gccaacgcgt
    28081 ctcggcgaac ggctttttaa gcccttatgc gatggatgcg ccagcactgg gtatggacgg
    28141 gccgtactat acggaagcca cagtcggttt gtttgccgga ctggatcttg atttgtgggg
    28201 tgtgcatcgc tcagcggtta ccgccgccat tggcgcgcat aatgccgcgc tggcagaaac
    28261 cgcagcagta gagctatcgc tgaccacggg cgtagcgcag ctttattaca gtatgcaggc
    28321 cagctatcag atgctcgatc tgttagaaca aactcgcgat gtgattgatt acgcggtgaa
    28381 agcgcaccaa agtaaagtgg cgcacggtct ggaagcgcaa gtgcctttcc acggcgcgcg
    28441 ggcacagatt ctggcggtcg ataaacaaat tgctgccgtt aaagggcaaa tcaccgaaac
    28501 gcgagaatct ctgcgtgcat tgattggcgc gggagccagc gatatgccgg agatcaaacc
    28561 agtggcatta ccacgagtcc agaccggtat tccggcgaca ctctcttatg agttgctcgc
    28621 cagacgcccg gatctgcaag ccatgcgctg gtatgttcag gcgtcattag atcaggtgga
    28681 ttccgcgcgg gcgctgttct acccaagctt tgatatcaaa gcgtttttcg gtctggactc
    28741 catccacctg gataccttat tcaaaaaaac cagtcgccag ttcaacttca tcccgggtct
    28801 gaaattgccg ctgtttgacg gtggacggtt gaatgccaat ctcgaaggca ctcgcgccgc
    28861 cagcaacatg atgattgaac gttacaacca gtcagtactg aacgcggtgc gtgacgttgc
    28921 cgtcaacggc acgcgtctgc aaacgctcaa cgacgagcga gaaatgcagg ctgaacgcgt
    28981 ggaagcaacg cgcttcaccc agcgcgctgc cgaggccgcc tatcagcgcg gcttaaccag
    29041 ccgcttacag gctaccgaag cccggttgcc ggtgcttgcc gaagagatgt cattactgat
    29101 gctggacagc cgccgggtga tccaaagcat tcagttgatg aaatcgctgg gcagcgggta
    29161 tcaggcggct cccgtcgtcg agaaaaaata aaatgtctgc cgtgtgatgg ctgtcacgcg
    29221 gtatttcgtt tcgtcacgtc aaaactgacg acagcctgtt tttcgtcaga gttttgaata
    29281 aatagtgccc gtaatatcag ggaatgaccc caaataaaat gtggcataaa agatgcatac
    29341 tgtagtcgag agcgcgtatg cgtgatttga ttaactggag cgagaccgat gaaaaaagtc
    29401 gtcacggttt gcccctattg cgcatcaggt tgcaaaatca acctggtcgt cgataacggc
    29461 aaaatcgtcc gggcggaggc agcgcagggg aaaaccaacc agggtaccct gtgtctgaag
    29521 ggttattatg gctgggactt cattaacgat acccagatcc tgaccccgcg cctgaaaacc
    29581 cccatgatcc gtcgccagcg tggcggcaaa ctcgaacctg tttcctggga tgaggcactg
    29641 aattacgttg ccgagcgcct gagcgccatc aaagagaagt acggtccgga tgccatccag
    29701 acgaccggct cctcgcgtgg tacgggtaac gaaaccaact atgtaatgca aaaatttgcg
    29761 cgcgccgtta ttggtaccaa taacgttgac tgctgcgctc gtgtctgaca cggcccatcg
    29821 gttgcaggtc tgcaccaatc ggtcggtaat ggcgcaatga gcaatgctat taacgaaatt
    29881 gataataccg atttagtgtt cgttttcggg tacaacccgg cggattccca cccaatcgtg
    29941 gcgaatcacg taattaacgc taaacgtaac ggggcgaaaa ttatcgtctg cgatccgcgc
    30001 aaaattgaaa ccgcgcgcat tgctgacatg cacattgcac tgaaaaacgg ctcgaacatc
    30061 gcgctgttga atgcgatggg ccatgtcatt attgaagaaa atctgtacga caaagcattc
    30121 gtcgcttccc gtacagaagg ctttgaagag tatcgtaaaa tcgttgaagg ctacacgccg
    30181 gagtcggttg aagatatcac cggcgtcagc gccagtgaga ttcgtcaggc ggcacggatg
    30241 tatgcccagg cgaaaagcgc cgccatcctg tggggcatgg gtgtaaccca gttctaccag
    30301 ggcgtggaaa ccgtgcgttc tctgaccagc ctcgcgatgc tgaccggtaa cctcggtaag
    30361 ccacatgcgg gtgttaaccc ggttcgtggt cagaacaacg tgcagggtgc ctgcgatatg
    30421 ggcgcgctgc cggatacgta tccgggatac cagtacgtaa aagatccggc taaccgcgag
    30481 aaattcgcca aagcctgggg cgtggaaagc ctgcctgctc ataccggtta tcgcatcagc
    30541 gagctgccgc accgcgcagc gcatggcgaa gtgcgtgccg cgtacattat gggcgaagat
    30601 ccgctacaaa ctgacgcgga gctgtcggca gtacgtaaag cctttgaaga tctggaactg
    30661 gttatcgttc aggacatctt tatgaccaaa accgcgtcgg cggcggatgt tattttaccg
    30721 tcaacgtcgt ggggcgagca tgaaggcgtg tttactgcgg ctgaccgtgg cttccagcgt
    30781 ttcttcaaag cagttgaacc gaaatgggat ctgaaaacgg actggcaaat catcagtgaa
    30841 atcgccaccc gtatgggtta tccgatgcac tacaacaaca cccaggagat ctgggatgag
    30901 ttgcgtcatc tgtgcccgga tttctacggt gcgacttacg agaaaatggg cgaactgggc
    30961 ttcattcagt ggccttgccg cgatacttca gatgccgatc aggggacttc ttatctgttt
    31021 aaagagaagt ttgacacccc gaacggtctg gcgcagttct tcacctgcga ctgggtagcg
    31081 ccaatcgaca aactcaccga cgagtacccg atggtactgt caacggtgcg tgaagttggt
    31141 cactactctt gccgttcgat gaccggtaac tgtgcggcac tggcggcgct ggctgatgaa
    31201 cctggctacg cacaaatcaa taccgaagac gccaaacgtc tgagtattga agatgaggca
    31261 ttggtttggg tgcactcgcg taaaggcaaa attatcaccc gtgcgcaggt cagcgatcgt
    31321 ccgaacaaag gggcgattta catgacctac cagtggtgga ttggtgcctg taacgagctg
    31381 gttaccgaaa acttaagccc gattacgaaa acgccggaat acaaatactg cgccgtgcgc
    31441 gtcgagccga tcgccgatca gcgcgccgcc gagcagtacg tgattgacga gtacaacaag
    31501 ttgaaaactc gcctgcgcga agcggcactg gcgtaataca gtcctttcta cagcctcctt
    31561 tcggaggctg tttttttatc cattcgaact ctttatactg gttactccct acccaatcgt
    31621 attatcaaaa tgaaaaaaat tatcgcattg atgttgtttt tgacattctt tgcccacgcc
    31681 aacgactccg agcctggcag ccagtattta aaggcagcag aggccgggga ccgacgcgca
    31741 caatattttc ttgccgacag ctggtttagc tccggcgatt tgagcaaagc cgaatattgg
    31801 gcacagaaag ccgccgacag cggtgatgct gatgcctgcg cgctgttggc gcagatcaaa
    31861 atcaccaatc cggtcagtct ggactatcca caagcaaaag ttcttgcaga gaaagcggcg
    31921 caagcgggca gtaaagaagg tgaagtaacg ctggcgcata ttctggtaaa tactcaagcg
    31981 ggtaaaccgg attatccaaa ggcgatttcg ctgttagaaa acgcctcgga agatctggag
    32041 aacgactctg ccgtcgatgc ccaaatgctg cttggtttga tttacgccaa cggcgtgggc
    32101 attaaggccg acgatgacaa ggcaacctgg tatttcaaac gcagctctgc aatttcccga
    32161 accggttatt ccgagtactg ggcgggaatg atgttcttaa acggtgaaga aggttttatc
    32221 gagaagaaca agcaaaaggc gctgcactgg ttgaacctga gctgtatgga agggtttgat
    32281 accgggtgtg aagagtttga aaaattaact aacggttaag gtaggcctga taagacgcgt
    32341 taagcgtcgc atcaggcgtt gatgtcggat gcagcgtaaa cgccttatca gacctacaaa
    32401 atcacgctag cgctgtctga cgtaggcctg ataagacgca ccagcgtcgc atcaggcatc
    32461 cacacattgc cgggggatat ccccggcaat cttcaattat tgatccgcag ttttatcaaa
    32521 tttgcccagc acttcacgct cataagccag cgcctttttg cggtcaaatt tgtgttccca
    32581 cttggcaatc accagcaccg ccagcgcatt acccaccacg ttcagtgcag tacgcgccat
    32641 gtcgaggata cggtcaacac cagcaataaa cgccagacct tccagcggaa tacctacgct
    32701 acccagcgtt gccagcaaca ccacaaacga cacgcccggc acgccagcaa tccctttcga
    32761 ggtcaccatc agcgtcagca ccagaatgat ttcctgccag atggacaggt caatgccata
    32821 cagctgagcg atgaaaatag cggcaatact ttgatacagc gtcgaaccat caaggttaaa
    32881 agagtaaccg gtcggcacca cgaagctggt gatcgacgcc ggtgctccgt aggcttccat
    32941 cttctcaata attcgcggca gtacgctttc agagctggca gtggagtacg ccagaatcag
    33001 ctcatctttc agaatacgaa tcaggatcca gacgcttaac ccgcataggc gcgccacaat
    33061 tcccagcact accagcgcga agaacagaat ggcgaaatgc accagcagca ccagtttcgc
    33121 cagtggccac agagaagaga aaccaaagtt agccaccgtc actgcaatca gcgcaaacac
    33181 acccaccggc gcataacgca tcaccatgtg agtcacttta aacatggttt cagagatgga
    33241 gcggaacacg gtcaccagcg gttcacgatg cgtcgcgggc agggaggaaa gccccagacc
    33301 aaacagcacc gagaagaaga tgatcggcaa catttcgcct ttcgccatcg acgccacaat
    33361 attcgtcggc accagcgaca aaatcgtgcc cataatgccg tgggaactgc tttgtaccgc
    33421 ttccgtagtg ctctgatatt tcgagatatc gacggtcgcc agctgcgaca tatccacccc
    33481 ggcaccgggc tggaagacgt tcgccagagt gatccccaaa atgatggcaa cggtggtgat
    33541 cacctcgaag tagataatgg ttttcgcgcc aatacgcccg agctgtttgg catcaccaac
    33601 acccgcgata cccaccacca gcgtggagat cacaatcggc acaacaatca ttttgatcag
    33661 atggatgaag atatcacccg ccggagagag caaattgacg accagccagt cgcggctgtc
    33721 gctatggtag tgcaggtagc ttcccaggag aatgcccagc accatagcaa acagaatctg
    33781 ccaggccagg ctgaatttta tatttttcat atgaatgact tcctcaatgg agcgccgttc
    33841 cccgttcaga aacgcgagaa cgtgacatac tgccagggca tgaattgtgt tgcgttggtt
    33901 tgtagatttt tttaacgctt atgatcagta tcatttgcgc ggtatgttgc gcaacccttt
    33961 aacaacagag cttttcgctg cattaatgca taataacgcg agtttatggc aggattaatg
    34021 aataaccatt tgttatggaa agatttttat ttgtttaatc tgcaataata ctgattagtg
    34081 attatttttg gcatatgttc attcgattat tttttatgca gctcaataac ataatcttca
    34141 tcaccagatg attaatccga tctccgctgc aacaatttcg ccatattaat cgactcgacc
    34201 agttgtgtcc ggttgatccg gggtgagttg agatccatca ctttttgcca tagttcgatg
    34261 gcttgcgcgt aattcgcctg cataaacgca tcggaagcca gcagcatcag agcggtgatt
    34321 tcgttagagt ccagcgcgag ggctttgtcg atcattgcgc gagtctgggc ggtcatatgc
    34381 tggctggcct ggtaataaag caccgtcgcc agcgccgcat acagttcagc gttctcacca
    34441 cgtagttgca acgcctgacg gtacgccagc agcgaattgc tgtaatcgtt ttgccacaga
    34501 tagtactcgc ccagtaacgc ccactgttcg ctgttttgtg gattagcgcg gattttatcc
    34561 tgcaatgcct gaagctgcgc ttccgggttt tgctggctgg caaactgatg tagcggatcg
    34621 cgctgacgct gatactccgc acgtaccgcc tgccattttg ggcttaacag ataactaccg
    34681 atacagagga ataccatcaa tatcgtgagt gttgtcagca ttttcaccgg tatttgaggc
    34741 tgtttcatcg cttcgccctc actcgccaga ggatcagtgc catcagcagt aacaacacca
    34801 ctggcagcgc ccacaacacc agcgtctgac ccgttaacgg tgggttatag cgaacaaaat
    34861 ctccgtagcg ttcggtcatc cagccaatga tttcgacttc acttttcccc tccgccacca
    34921 tgctgtaaac ctgatggcgc atactgactg ccaccggcgc gttggattcc agtaagtttt
    34981 gattctggca ctgcggacaa cgtaactggc tggcaatatt taacgcctgt tgctgttgtt
    35041 gcggattggc gaattgccag gtgtctacaa cctgagcgcg cgcaaaacag gtaaataaca
    35101 gcagtagcgt gagaagccct ttatacatcg cgcttcttcc cccgccatcc gcttaacaat
    35161 gcgcccgcaa tcatcaacaa tcctcccccc cagatccagc gcacaccgct ttgtacatac
    35221 aagcgaaaag cgtaacgatc cgctccagtt ttctccccca tgaccgcata ccagtcatgg
    35281 atgccgttcc agcgaattga cggttccatc atttgctgac gacgagcttc gtaaaaacgc
    35341 cgctccggcg ttaattcacc aatgcgttgc tgatggtcaa acagcgccac tatcgctttt
    35401 tcgctggtgt aattgccttt ggcttgcaga tcgaggcgct caaaacggaa ggtgtatcct
    35461 gccagcgtca cctgctgacc cggctgtaaa ttcaggctga tctcctgacg gctgacgctg
    35521 gagacaacga tccctgcggc aaataacagt atgcccgcat gcgccagcag cgccggaagc
    35581 tgcgcgcgtt tgccagagac aaacgtcgcc agcacaatca ccaccaacat caacagacca
    35641 aacggtaacg tcgcgcggtt aaaatacggt gcgccaacgg agagacgtcc ccagcccagc
    35701 aggccgtaaa tcatcggata aagcgttccc accagcacga tcagtagcac tgcgcaaaac
    35761 agcaacagcg tagcgaggat taacatttcc cgcgataacc ccgaaaaacg caccgccggg
    35821 ccaccgtccc gcgctcgcca gccatacaga gccagagacg caaggctaat cagtgcaaac
    35881 aggctgaaca acggcacagc gcgaacgtta tccagagcga acgcatgaac cgaaaccaga
    35941 atgccagaac ggacaattaa ggtgcccagc agcgacagca tcagagtaac tatcgccaac
    36001 aacagcgacc agtggcgaaa aatcccccgc tggcgtgtca gggataaact gtgcagcagc
    36061 gcagtggcag aaagccaggg taataaagag gcgttttcca ccggatccca gaaccaccag
    36121 ccgccccagc cgagttcgca gtaggcccac caggaaccga ggatgatccc cgcagttaat
    36181 gcactccagc caggtaacgc ccagcgccag caaattcggg cgcaggcacc atcaaactcg
    36241 ccgcgcagta gactcgccag cgccacgctc gccgctacca tcaaaccgcc atagccaagg
    36301 taaagcagcg gtggatgaaa gataagaccg ggatgttgca gcatcggatt gagatcgcgg
    36361 ccttcgattg ctggtggaaa tatccgcacg aagggatcgg accacagcac tacaaacagt
    36421 agcagtgcgg cgagcatgag agataaaacg gctagcgtca gcggaaatag cggatcggtt
    36481 tgctgccgat attgccaggc aaacagcgcg ctccaggcgg aaagcagcag cacccaaagc
    36541 agcagcgaac cttcatgacc gccccacacc gccgccagtt tgagtttcca cgacagcaag
    36601 ctgtagctat gttgggcgac ataaatgacc gagaaatcgc tgatgagaaa acaatacgtc
    36661 agtacgccaa aggcgagcag caggagcgcg aactgtgcca gaatgccgat gcaagtgagt
    36721 cgcatcatgg caggccagcg caaccgcact cccgcaaagg ccgtcaacgg ggtcaacacg
    36781 ttgaccccga gacttaacaa cagcgccaga aaaccgactt caggaaggaa tacgtccaga
    36841 aaccacctct gttctggctt acgccaccgt taactgcccg gcgtagagaa tgaaaaatcg
    36901 caacatcaac acgccgacca gactggcccc acacgccgcc agcacggcag gaatgccgga
    36961 actgcgattg acccacggtt tgaacaacat tggcacaatc agccccagcc cggcgacacc
    37021 aagccagaac caccacgtcc agaaaccgcc gcccaatgcc gccaccagcg cacgcacttt
    37081 accgtcatcg cccagcgcca gaccgacaaa aaacgccgcc agcaggaaga tttcacccca
    37141 taccacgggg atttccatac ggtgtacaaa ctgcgcttcc gtggaatgcg ggttactgcg
    37201 ttggcgtatc gccatggcga tcagtgccac cgccgcaccg gacgagatgc cggagaagag
    37261 gaacagcacc ggcaggatcg ggttattaag gaacggatac gatttcagcg ccgacagcag
    37321 aaaaccggta taagccccca acaacaccgc cagcaccagc atcaatgttt ccagtccgcg
    37381 atgaacgggc gtcagcaggc tcagaacctt ttgcacgatc cccagcttcg gcaaccagcg
    37441 ttgttgcagg gcaagcaaat catgttcaaa gattttcgcc agccacagca ccagcaccac
    37501 catgtaaagc tgaaacagca tcacccccat cgacatcacc gaagtaaaac tgtagtggaa
    37561 catcagcttc cagaaggtcc acggtcttgt caggtggaag acgaggctca acagaccgag
    37621 gatcaccgcg ccaggtccga caatcagcgt ggtgcgcagc aacgtgctgt ctgcaccgcc
    37681 cgcctgcggg tagaagcgac gtaacagcac ggccagcgtc accagaccgg cagaaatacc
    37741 aatcaaaaac aggtagatgg caatcggcca gtcccacacc agcgattcaa aatgaaatgc
    37801 ggaagtctgc gtcattggct cacctccccg tatttaaacg gtacgcggta cagcttcggt
    37861 ttggttccca gcgccagctt gtagcggtaa gtgggcttct ggcgcagcag ttgcgaaatc
    37921 tcactgttgg gatcgtccag attgccaaac gtcagcgcct tcgtcgggca agcctcaacg
    37981 cacgcgggaa gcttaccggc ctgcaaattg gtcttacggc agaaatcgca tttgtccgct
    38041 gtcttcgtga ccggatgaat aaagcgcacg cggtacggac aggcggcgat gcagtactga
    38101 caaccgacgc agagatccgg attaacatcg acaatcccac tggcagcatc gcgaaaagat
    38161 gcaccggtcg ggcagacgtc aacgcacggc gcatgatcgc agtgctggca agacttacgg
    38221 aagaagcgat atttcacatc aggaaattcg ccctgcggct cgctacgaat aatcgtcagg
    38281 cgcgagacgc cttccggcac cttgttcact tcccgacaag catccataca ggcggtacag
    38341 ccgatgcata acgactcgtc atgcaccatg ccgtaacgca ccccattgat attcaacgtt
    38401 ttcgccacca cgcgccctgc ggtgccgctg acggctgcca gcacgccgac gccggtgaga
    38461 aactggcgac gagaccaggt catggctgct ccttaagcaa cggaacagac gccgggttaa
    38521 agttcggatt ggtgcgctga tcgctgtggc aatcgacgca aatcttaatc cgtcctttgt
    38581 cacttaacgt ctgcatcgta tcttgctgtg gatgcaggga atggcagctg gcgcacgcca
    38641 ctttggttac gtggacatcg tgcggccaga acgctttttg caactgttcc ggcagatggc
    38701 aggacataca gacgctgttc tgctctccaa ccttgtacat cggctcgtta aagcgcatca
    38761 catctttcac cccttcgcgg tgttgcggtg atggctggcc gtgacagttg gtgcaggtga
    38821 ccggcagttt gttattcggg ttgataacgg aagcatgttt gccatgcata ccttcggtat
    38881 ctggcttatg acagtccaga caggcggcat ccgggttacg ctgctgggta acttcgtagc
    38941 gatcatcaga tgcctgcgcc gcaggggtag cggtaattcc gttcaggctc cacaacaggc
    39001 ccgacgccag caccccggca gttaataacg aacgtaatac gctcatattc actccattgt
    39061 gttttccgcc ccttgcgagg cggaacgggg ttattggctt aacagaccgt ttttacgtgc
    39121 ctgctcttcc cactgcggga tcaccgtttt gatgaagtcc tgcttctcgg ccttgatttg
    39181 ttccatgttc aggccaatgg cctgctgggc tttctctttg gtcgagatat ccgggatctg
    39241 gatttcatgg gtgatgcctt tagtcgccag caggcgcgcc agtttggtgc gtgcatccgc
    39301 cgctttatcc atcgccgtac cgagcatcct cagaccttct tccggtgcgt gcatatgaat
    39361 gccgtgggaa gcgatcgcca gatcccaacg ccactgggca tgacggatat cgtcctgaat
    39421 cggcttcatt tcggcttccg tcgcacctgc gtccagcgcc gctttcgctt cgaagtgagc
    39481 gtgaaccagt tgatcttcaa ccttgatttt caggtcgtta atcgactgct tacgttccgc
    39541 gaccactttt tgcaaggcag ctttgtcctg ggtatggcag ttcgcacaag tctgggcgaa
    39601 gttatcaaac ggattaccaa ttttatggtc ggtgtagagt ttgccttcgg cgttctgcac
    39661 ttttggcata tggcagtcga tacaggtcac gttgttttta ccgtgaatgc ccgctgtcca
    39721 ggtttcatat tccgggtgct gcgctttcag cattggcgtt ttcgacaggg agttagtcca
    39781 gtcagagaag gcaattttgt cgtaatactg ctccatattt tcgactttca tgccgtcatc
    39841 ccacgggaat ttaaccgctt tgtttttgcc gtcgaagtaa tactccacat ggcactgacc
    39901 gcaaaccatc gattgctggt cgaaacgtcc ggctttctca aaaggtttac caatggcttc
    39961 catcgcgcga gccgcatacg gacgggaaag ggttaactcc ggtttgcctt tggcgaactc
    40021 tggagaggcg gtgttatggc aatcggcaca acctaagttg ttgacgattt ccgggccgcc
    40081 gcgcgcccat ttaccgtgga agtagccatc ttcgccgtct ttctggatca gacgcgccac
    40141 atccgggctt ttacaactcc agcacgccat cggtagcgga ccatcttcag cgtttttcgg
    40201 cgcaccggta cgcagggttt cacgcacatc ggtcacagca aaggcatgtc cacgcggctt
    40261 gttgtaatcg cgcgagaagg gataccccgc ccacaggatc accagccgtg gatcttccgc
    40321 cagggcgtca acacgctctg actgttccga ggtggctttc caggagagat attgatcggg
    40381 atgctgcggg gcaaaggttt cattcttcgc ttccacagtt acaggttttg cgggagcagc
    40441 cgtttgttca gcgtgaacag aagtgaaaaa gaaaaaagga atcaataagc tgaagatacg
    40501 gcgtgcgttt atttttatcc ttgtcatagg ggcttcatcc gaattgcgcc attgttgcaa
    40561 tggcggtttt tattgttttt cacgacagta accgcaccta cactgtcatg acattgctcg
    40621 cccctaagtg taacaaataa ccacactgtg aatgtcgtct ttaatcaatt gtaagtgcat
    40681 gtaaaatacc actttagagt tagtcagtat cttcctcttt ttcaacagca tgcataactg
    40741 catgttcctc aaagaattaa tcaacttttg ttgctgacct tcaaagatta ccctgccgtt
    40801 tatttgcaca attctacttt tgcgtgatct gtcgcccaaa tactaaacaa aactgccaat
    40861 acccctacat ttaacgctta tgccacatat tattaacatc ctacaaggag aacaaaagca
    40921 tgagtcaaat tcacaaacac accattcctg ccaacatcgc agaccgttgc ctgataaacc
    40981 ctcagcagta cgaggcgatg tatcaacaat ctattaacgc acctgatacc ttctggggcg
    41041 aacagggaaa aattctcgac tggatcaaac cgtaccagaa ggtgaaaaac acctcctttg
    41101 cccccggtaa tgtgtccatt aaatggtacg aggacggcac gctgaatctg gcggcaaact
    41161 gccttgaccg ccatctgcaa gaaaacggcg atcgtaccgc catcatctgg gaaggcgacg
    41221 acgccagcca gagcaaacat atcagctata aagagctgca ccgcgacgtc tgccgcttcg
    41281 ccaataccct gctcaagctg ggcattaaaa aaggtgatgt ggtggcgatt tatatgccga
    41341 tggtgccgga agccgcggtt gcgatgctgg cctgcgcccg tattggcgcg gtgcattcgg
    41401 taattttcgg tggcttctcg ccggaagcgg ttgccgggcg cattatcgat tccaactcac
    41461 gactggtgat cacttccgac gaaggcgtgc gcgccgggcg tagtattccg ctgaagaaaa
    41521 acgttgatga cgcactaaaa aacccgaacg tcaccagcgt agagcatgtg gtggtactga
    41581 agcgtactgg cgggaaaatt gactggcagg aagggcgcga cctgtggtgg cacgaccagg
    41641 ttgagcaagc cagcgatcag caccaggcgg aagagatgaa cgccgaagat ccgctgttta
    41701 ttctctatac ctccggttct accggaaaac caaaaggcgt actgcacact accggcggtt
    41761 atctggtgta cgcggcgctg acctttaaat atgtctttga ttatcatccg ggcgatatct
    41821 actggtgcac cgccgatgtg ggctgggtga ccggacacag ttatttgctg tacggcccgc
    41881 tggcctgcgg cgcgaccacg ctgatgtttg aaggcgtacc gaactggccg acgcctgccc
    41941 gtatggcaca ggtggtggac aagcatcagg tcaatattct ctataccgcg cccacggcga
    42001 ttcgcgcgct gatggcggaa ggcgataaag cgatcgaagg caccgaccgt tcgtcgctgc
    42061 gcattctcgg ttccgtgggc gagccaatta acccggaagc gtgggagtgg tactggaaaa
    42121 aaatcggcaa cgagaaatgt ccggtggtcg atacctggtg gcagaccgaa accggcggtt
    42181 tcatgatcac cccgctgcct ggcgctaccg agctgaaagc cggttcggca acacgtccgt
    42241 tcttcggcgt gcaaccggcg ctggtcgata acgaaggtaa cccgctggaa ggggctaccg
    42301 aaggtagcct ggtgatcacc gactcctggc cgggtcaggc gcgtacgctg tttggcgatc
    42361 acgaacgttt tgagcagacc tatttttcca ccttcaaaaa tatgtatttc agcggcgacg
    42421 gcgcgcgtcg tgatgaagat agctattact ggatcaccgg gcgtgtggac gatgtgctga
    42481 acgtctccgg tcaccgtctg ggaacggcgg agattgagtc ggcgctggtg gcgcatccga
    42541 aaatcgccga agccgctgtc gtcggtattc cgcacaatat taaaggtcag gcgatctacg
    42601 cctacgtcac gcttaatcac ggggaggaac cgtcaccaga actgtacgca gaagtccgca
    42661 actgggtgcg taaagagatt ggcccgctgg cgacgccaga cgtgctgcac tggaccgact
    42721 ccctgcctaa aacccgctcc ggcaaaatta tgcgccgtat tctgcgcaaa attgcggcgg
    42781 gcgataccag caacctgggc gatacctcga cgcttgccga tcctggcgta gtcgagaagc
    42841 tgcttgaaga gaagcaggct atcgcgatgc catcgtaacc cacaattgcc ggatgcgagt
    42901 cggtaacggt ttgtaggcct gataagacgc gacagcgtcg catcaggcat tgattgccgg
    42961 atgcggcgta taacgcctta tccggcctac attcggcaag ggttacccga gcgttaacct
    43021 tctcccataa gggagcggga attaaaacaa tccctacatt acctctggag aatctgtgat
    43081 gaatggcact atttatcagc ggatagaaga caatgcgcat ttcagggagt tagtcgaaaa
    43141 acggcaacgg tttgccacca tcctgtcgat tattatgctg gcagtttata tcggctttat
    43201 tttactgatc gccttcgcgc ccggctggct gggcaccccg ctgaatccga acaccagcgt
    43261 cacacgcggt attccagtcg gtgttggggt aatagtgatc tcctttgttc tcactggtat
    43321 ctacatctgg cgggcgaacg gcgaattcga ccgtcttaat aatgaagtcc tgcatgaggt
    43381 acaagcatca tgaaaagagt tctgacggcg cttgccgcca cactcccttt cgcagctaac
    43441 gccgcggatg ctattagcgg ggccgtagag cgccagccaa cgaactggca ggcgattatt
    43501 atgttcctga ttttcgtcgt gtttacgctc ggcattacct actgggcgtc aaaacgcgta
    43561 cgttcgcgta atgactatta caccgcaggc ggcaatatca ccggttttca gaacgggctg
    43621 gcgattgccg gtgactatat gtccgccgcc tcatttttgg ggatctccgc actggtgttt
    43681 acctccggct atgacggctt aatttactcg ctgggcttcc tggtgggctg gccgatcatt
    43741 ttgttcctga ttgccgaacg tctgcgtaac ctggggcgtt acacctttgc cgatgtagcc
    43801 tcttatcgtc tgaaacaagg gccgattcgt attctttcgg cctgtggttc tctggtggtg
    43861 gtggcgcttt accttatcgc ccagatggtg ggtgcaggta aactgatcga gctgctgttt
    43921 ggccttaact atcacattgc agtggtgctg gtcggcgtgc tgatgatgat gtacgtcctg
    43981 ttcggcggca tgctggcgac cacctgggta cagattatca aagctgtgct gttgctgttc
    44041 ggtgccagct ttatggcctt tatggtgatg aaacacgtcg gctttagctt caacaatctg
    44101 ttcagcgaag cgatggcggt acacccgaaa ggtgtcgaca tcatgaaacc gggcggactg
    44161 gtgaaagatc cgatctccgc actctctctg gggctaggac tgatgtttgg tacggctggc
    44221 ttgccgcaca ttctgatgcg cttctttacg gtcagcgatg cccgcgaagc gcgtaaaagc
    44281 gtgttctacg ctaccggatt tatgggctac ttctatatcc tgacctttat tatcggcttc
    44341 ggcgcgatca tgctggtcgg tgcgaatccg gaatataaag acgcggcggg ccatctgatt
    44401 ggtggtaaca acatggcagc agttcacctg gccaatgcgg tgggcggcaa cctgttcctc
    44461 ggctttattt cagcggttgc tttcgccact atcctcgcgg tggttgcggg tctgacgctg
    44521 gcgggcgcat cagcggtttc gcatgacctg tacgctaacg tcttcaaaaa aggcgcgacc
    44581 gaacgtgaag agctgcgggt atcgaaaatc accgtcctga ttttgggtgt gattgcgatt
    44641 atcctcggcg tgctgtttga gaatcagaac atcgccttta tggtggggct ggcgtttgcc
    44701 atcgcggcga gctgtaactt cccgatcatt ctgctttcta tgtactggtc gaaactgacc
    44761 acgcgtggcg cgatgatggg tggctggctg gggctgatta ccgcagtggt actgatgatc
    44821 ctcggcccga cgatttgggt acagatcctc ggtcacgaaa aagccatctt cccgtatgaa
    44881 tacccggcgc tgttctctat caccgtggca ttcctcggca tctggttctt ctcggcaacc
    44941 gataactcag cggaaggcgc gcgtgagcgt gaactgttcc gcgcgcagtt tatccgctcc
    45001 cagaccggct ttggcgttga gcaaggccgc gcgca
//
LOCUS       DAZFAB010000027        44358 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZFAB010000027 DAZFAB010000000
VERSION     DAZFAB010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.027"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..185
                     /locus_tag="ACHJWA_001770"
     CDS             <1..185
                     /locus_tag="ACHJWA_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO4308392.1"
                     /translation="TPDEQKEPVAAIAPSLSTSTQTRVSASSCKVEFRHGNMTLENPS
                     PELLTMLIRELSGRGR"
     gene            182..532
                     /gene="tnpB"
                     /locus_tag="ACHJWA_001771"
     CDS             182..532
                     /gene="tnpB"
                     /locus_tag="ACHJWA_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311895.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO4308393.1"
                     /translation="MISLPSGTRIWLVAGVTDMCKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            563..2148
                     /locus_tag="ACHJWA_001772"
                     /pseudo
     CDS             563..2148
                     /locus_tag="ACHJWA_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            2177..2320
                     /locus_tag="ACHJWA_001773"
                     /pseudo
     CDS             2177..2320
                     /locus_tag="ACHJWA_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2431..2738
                     /locus_tag="ACHJWA_001774"
                     /pseudo
     CDS             2431..2738
                     /locus_tag="ACHJWA_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612262.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2735..3970)
                     /gene="pitB"
                     /locus_tag="ACHJWA_001775"
                     /pseudo
     CDS             complement(2735..3970)
                     /gene="pitB"
                     /locus_tag="ACHJWA_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
     gene            complement(4262..6121)
                     /gene="gss"
                     /locus_tag="ACHJWA_001776"
     CDS             complement(4262..6121)
                     /gene="gss"
                     /locus_tag="ACHJWA_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFO4308394.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVVHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKE"
     gene            6326..7192
                     /gene="yghU"
                     /locus_tag="ACHJWA_001777"
     CDS             6326..7192
                     /gene="yghU"
                     /locus_tag="ACHJWA_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFO4308395.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(7315..7563)
                     /gene="hybG"
                     /locus_tag="ACHJWA_001778"
     CDS             complement(7315..7563)
                     /gene="hybG"
                     /locus_tag="ACHJWA_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFO4308396.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(7576..7917)
                     /gene="hypA"
                     /locus_tag="ACHJWA_001779"
     CDS             complement(7576..7917)
                     /gene="hypA"
                     /locus_tag="ACHJWA_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO4308397.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEVHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(7910..8398)
                     /gene="hybE"
                     /locus_tag="ACHJWA_001780"
     CDS             complement(7910..8398)
                     /gene="hybE"
                     /locus_tag="ACHJWA_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFO4308398.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHALSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(8391..8885)
                     /gene="hybD"
                     /locus_tag="ACHJWA_001781"
     CDS             complement(8391..8885)
                     /gene="hybD"
                     /locus_tag="ACHJWA_001781"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFO4308399.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKSAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(8885..10588)
                     /gene="hybC"
                     /locus_tag="ACHJWA_001782"
     CDS             complement(8885..10588)
                     /gene="hybC"
                     /locus_tag="ACHJWA_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFO4308400.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(10585..11763)
                     /gene="hybB"
                     /locus_tag="ACHJWA_001783"
     CDS             complement(10585..11763)
                     /gene="hybB"
                     /locus_tag="ACHJWA_001783"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFO4308401.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(11753..12739)
                     /gene="hybA"
                     /locus_tag="ACHJWA_001784"
     CDS             complement(11753..12739)
                     /gene="hybA"
                     /locus_tag="ACHJWA_001784"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFO4308402.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(12742..13860)
                     /gene="hybO"
                     /locus_tag="ACHJWA_001785"
     CDS             complement(12742..13860)
                     /gene="hybO"
                     /locus_tag="ACHJWA_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFO4308403.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(14049..14336)
                     /gene="yghW"
                     /locus_tag="ACHJWA_001786"
     CDS             complement(14049..14336)
                     /gene="yghW"
                     /locus_tag="ACHJWA_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFO4308404.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(14455..15342)
                     /gene="yghX"
                     /locus_tag="ACHJWA_001787"
     CDS             complement(14455..15342)
                     /gene="yghX"
                     /locus_tag="ACHJWA_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFO4308405.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIFAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLNSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            15499..16539
                     /gene="gpr"
                     /locus_tag="ACHJWA_001788"
     CDS             15499..16539
                     /gene="gpr"
                     /locus_tag="ACHJWA_001788"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFO4308406.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPEDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDERVTSVLVGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(16579..17073)
                     /gene="yqhA"
                     /locus_tag="ACHJWA_001789"
     CDS             complement(16579..17073)
                     /gene="yqhA"
                     /locus_tag="ACHJWA_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFO4308407.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            17264..18148
                     /gene="yghA"
                     /locus_tag="ACHJWA_001790"
     CDS             17264..18148
                     /gene="yghA"
                     /locus_tag="ACHJWA_001790"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO4308408.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(18420..18845)
                     /gene="exbD"
                     /locus_tag="ACHJWA_001791"
     CDS             complement(18420..18845)
                     /gene="exbD"
                     /locus_tag="ACHJWA_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFO4308409.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(18852..19586)
                     /gene="exbB"
                     /locus_tag="ACHJWA_001792"
     CDS             complement(18852..19586)
                     /gene="exbB"
                     /locus_tag="ACHJWA_001792"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO4308410.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            19838..21025
                     /gene="metC"
                     /locus_tag="ACHJWA_001793"
     CDS             19838..21025
                     /gene="metC"
                     /locus_tag="ACHJWA_001793"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFO4308411.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            21165..21824
                     /gene="yghB"
                     /locus_tag="ACHJWA_001794"
     CDS             21165..21824
                     /gene="yghB"
                     /locus_tag="ACHJWA_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFO4308412.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(21864..22763)
                     /gene="yqhC"
                     /locus_tag="ACHJWA_001795"
     CDS             complement(21864..22763)
                     /gene="yqhC"
                     /locus_tag="ACHJWA_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFO4308413.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMARPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            22957..24120
                     /gene="yqhD"
                     /locus_tag="ACHJWA_001796"
     CDS             22957..24120
                     /gene="yqhD"
                     /locus_tag="ACHJWA_001796"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311921.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFO4308414.1"
                     /translation="MNNFNLHTPTRILFGKSAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLGVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            24225..25052
                     /gene="dkgA"
                     /locus_tag="ACHJWA_001797"
     CDS             24225..25052
                     /gene="dkgA"
                     /locus_tag="ACHJWA_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFO4308415.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     FDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHNRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            25252..26174
                     /gene="yqhG"
                     /locus_tag="ACHJWA_001798"
                     /pseudo
     CDS             25252..26174
                     /gene="yqhG"
                     /locus_tag="ACHJWA_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
     gene            26223..26480
                     /gene="yqhH"
                     /locus_tag="ACHJWA_001799"
     CDS             26223..26480
                     /gene="yqhH"
                     /locus_tag="ACHJWA_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFO4308416.1"
                     /translation="MKTIFTVGAVVLATCVLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(26523..28742)
                     /gene="ygiQ"
                     /locus_tag="ACHJWA_001800"
     CDS             complement(26523..28742)
                     /gene="ygiQ"
                     /locus_tag="ACHJWA_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFO4308417.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQCQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(28853..30265)
                     /gene="ftsP"
                     /locus_tag="ACHJWA_001801"
     CDS             complement(28853..30265)
                     /gene="ftsP"
                     /locus_tag="ACHJWA_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFO4308418.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(30340..31077)
                     /gene="plsC"
                     /locus_tag="ACHJWA_001802"
                     /gene_synonym="parF"
     CDS             complement(30340..31077)
                     /gene="plsC"
                     /locus_tag="ACHJWA_001802"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFO4308419.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(31311..33569)
                     /gene="parC"
                     /locus_tag="ACHJWA_001803"
     CDS             complement(31311..33569)
                     /gene="parC"
                     /locus_tag="ACHJWA_001803"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFO4308420.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(33707..35314)
                     /gene="ygiS"
                     /locus_tag="ACHJWA_001804"
     CDS             complement(33707..35314)
                     /gene="ygiS"
                     /locus_tag="ACHJWA_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO4308421.1"
                     /translation="MYTRNLLWLVSLVSAAPLYAADVPTNTQLAPQQVFRYNNHSDPG
                     TLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEILDGGRRYIFHLRSGLQ
                     WSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGVKAT
                     DDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDQWV
                     VNEKITARKNPKYRDAQHTVLQQVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIE
                     KSLPGEIRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATT
                     LTPPEVKGFSATTFDELQKPMSERVAMAKALLKQAGYDASHPLRFELFYNKYDLHEKT
                     AIALSSEWKKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASSFLNTLKS
                     DSEENVGHWKNAQYDALLNQAMQITDATKRNALYQQAEVIVNQQAPLIPIYYQPLIKL
                     LKPYVGGFPLHNPQDYVYSKELYIKAH"
     gene            complement(35423..35905)
                     /gene="ygiV"
                     /locus_tag="ACHJWA_001805"
     CDS             complement(35423..35905)
                     /gene="ygiV"
                     /locus_tag="ACHJWA_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFO4308422.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCRPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETNVYVPLA
                     "
     gene            complement(35958..36350)
                     /gene="ygiW"
                     /locus_tag="ACHJWA_001806"
     CDS             complement(35958..36350)
                     /gene="ygiW"
                     /locus_tag="ACHJWA_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFO4308423.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            36502..37161
                     /gene="qseB"
                     /locus_tag="ACHJWA_001807"
     CDS             36502..37161
                     /gene="qseB"
                     /locus_tag="ACHJWA_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFO4308424.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALEERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            37158..38507
                     /gene="qseC"
                     /locus_tag="ACHJWA_001808"
     CDS             37158..38507
                     /gene="qseC"
                     /locus_tag="ACHJWA_001808"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFO4308425.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGDKDQWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPVMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNVQGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            38617..39199
                     /gene="mdaB"
                     /locus_tag="ACHJWA_001809"
                     /pseudo
     CDS             38617..39199
                     /gene="mdaB"
                     /locus_tag="ACHJWA_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
     gene            39230..39544
                     /gene="ygiN"
                     /locus_tag="ACHJWA_001810"
     CDS             39230..39544
                     /gene="ygiN"
                     /locus_tag="ACHJWA_001810"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFO4308426.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(39589..40476)
                     /locus_tag="ACHJWA_001811"
     CDS             complement(39589..40476)
                     /locus_tag="ACHJWA_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFO4308427.1"
                     /translation="MIQKDKTRVDIYGERFRTRASQLTPGLRAVASYINEHREVVLDQ
                     TAMEIAATLNTSDATVIRAIQALGFAGLRDLKRTLEQWLGPALSSSEKMSTTVSNLTS
                     DVNTAIDFVLEGHLYTCNVLSEPENRHALAQAVALLVQARQVAIFGIGASGILADYTA
                     RLFNRIGLPATALNRTGIGLAEQLIALQRGDVLIMMAQKSAHREGLTTLREARRLGIP
                     VILLTNALDSRFSKDASIVIHVPRGDEKGKTPLHGTVLLCLEMIVWSVASAVPQRAVK
                     TIKRINDFHRGLKTGRKNG"
     gene            complement(40473..41420)
                     /locus_tag="ACHJWA_001812"
     CDS             complement(40473..41420)
                     /locus_tag="ACHJWA_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO4308428.1"
                     /translation="MRLFFSLLILLSFFARATEPVQVFTDDLGRKVTVPAHPKRIVSL
                     HDLDITIPLIELGVPPVASHGRTRPDGSHFIRSGALLTGVDFDNSSIAFIGTADIDIE
                     AIVAAKPDLIITEPTRNTPIERLEKIAPTVSIDHLKGGAPEIYRKLAELTGTQSQLAI
                     LERRYQAQINALKATLDSQKITVSVIQANQGKINVMHSYHSLGRVLRDAGFRFPPLIE
                     SIPEGGRMDVSAERLPELDADFVFATWRGDTGGKPQDELAAMEKVMPGWCQFLTACRS
                     GRYVLISREEAISNSFASLGLMAAQIQSQIAGRPLPEAK"
     gene            complement(41431..42468)
                     /locus_tag="ACHJWA_001813"
     CDS             complement(41431..42468)
                     /locus_tag="ACHJWA_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFO4308429.1"
                     /translation="MNVGLRPLRVGKFSTLVRPKNLVLLGGLFLFAVGILIFGLMHGS
                     FFVPASEVGRALFAPENVSTDARYIVQDIRLPRVIMALLCGAMLGMAGAAMQSIARNG
                     LADPGLIGVKEGCSVAVLWLIFQFPMLGMFWRPVAGLAGGLLVALIVIFCAREISRPR
                     FVLIGIGVSWFFAAGIGVFMTTADVRDVQTALMWLSGSLHAANWMLVGISACWMLPAA
                     LLLLFTARTADIALLGHQVATGLGVNSSRLALLRVAAPIILTAVCVSCVGNIGFVGLI
                     APHISRFILRGGQTTLLLGSAVSGALLVILADSIGRLAFLPLQLPAGIIISLIGGPFF
                     LLLLWQRRNSF"
     gene            complement(42477..43460)
                     /locus_tag="ACHJWA_001814"
     CDS             complement(42477..43460)
                     /locus_tag="ACHJWA_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFO4308430.1"
                     /translation="MMRTGLVTVIFLALLLVGCVVYPGIGARFIAPQTVLQAFLHFEP
                     QNFDHNVIVRLRLPRLAAALLTGASLGVAGALLQAVIRNPLGEPHILGLNAGAALAVV
                     AASALGLAFPVGRPLLASTGGALLFLLILLLSSAGRSGLTPMKVTLCGVALSAFVSSI
                     TAAILILDEQTLLAMRTWLAGDLAGQDWATLGTSAWFSLGGVVLAIYLAPSLNMLALG
                     DRMAQGLGVSVLRTRTFTLLAIALLCGAAVSIAGPIGFVGLLVPQIVRRLVSADLRVL
                     LPLSACVGALLLLLADIIARTLFTPHELATGVMTALVGAPVFVIMATRMFK"
     gene            complement(43457..44266)
                     /locus_tag="ACHJWA_001815"
     CDS             complement(43457..44266)
                     /locus_tag="ACHJWA_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO4308431.1"
                     /translation="MAHNAKGQGLILDNVSAGYHKKIIVDGVSFSVPTEKMTVLVGAN
                     GCGKSTLLSTIARVLQPMGGSILLDGKAIHEQPTKALSRRLGILPQSPLLPEGLTVYE
                     LVSRGRFPWQNFIRQWSDADEQAVEEALKLTGTQEFAHLPVEKLSGGQRQRCWIAMVL
                     AQKTPYILLDEPTTWLDLRYQVEILELLHDLTRHHGRTVVVVLHDLNFAVNYGDTLIF
                     LRQGKVVRVLNEGEHCTPELVKAVFDVDVHASINTLTGKPFFMPFRGVEKV"
BASE COUNT        11137 a        11856 c        11170 g        10195 t
ORIGIN      
        1 taacgccgga tgagcagaaa gaacctgtgg cggccattgc gccgtcttta tccacttcca
       61 ctcagaccag agtcagtgcc agttcctgca aggtggaatt ccgtcacggt aacatgacgc
      121 tggaaaatcc atcgccagag ctgctcacga tgttaatccg cgaactgagc gggaggggaa
      181 gatgatttca ctcccatcag gtacccgtat ctggctcgtt gccggcgtta ccgatatgtg
      241 taaatccttc aacggactgg gagaacaggt acaacatgtg ctgaatgata atcccttctc
      301 cggtcacctg tttatcttcc gtggccgacg gggggacacc gttaaaattc tttgggctga
      361 tgctgatggt ctgtgcctgt tcaccaaacg cctggaggaa ggccagttta tctggcctgc
      421 ggtgcgtgac ggtaaggtat ccattacccg ctcgcaactg gcaatgctcc tcgataagct
      481 ggactggcgt cagccaaaaa catcccgcct taacgccctg acaatgttgt aaaaaacacc
      541 tggccgcatt ataaaaacgg ccatgagtca gaaatacctc attcgcattg cagaactgga
      601 aagccagctc cgtcagaaag accagcaact gagtctggtt gaagagacgg aggccttcct
      661 gcactggcac gtgccgaaga aaagatcgaa gaagatgaac gggaaataga acatctgcgg
      721 gctcagatag aaaaactgcg ccggatgctg ttcggtaccc gttctgaaaa actgcgtcgt
      781 gaagttgaac aggctgaggc cctgctgaaa caacgcgaac aggacagcga tcgttacagt
      841 gggcgggaag acgatccaca ggttccccgc cagttgcgac agtcgcgcca tcgtcgtccg
      901 ttaccggcac accttccccg tgaaatacag cgtctggaat ctgaagaaag ctgttgcccg
      961 gagtgtggcg gtgagctgga ttatctgggg gaagtcagtg cagaacaact ggaactggtg
     1021 agcagcgccc tgaaagtgat ccgcacagaa cgggtaaaaa aagcgtgtac aaaatgtgac
     1081 tgcatcgttg aagcaccggc gccgtcccgc ccgatagagc gtggtatcgc gggccccgga
     1141 ttacttgccc gcgtgttaac gggaaaatac tgcgaacacc tgccactgta tcgtcagagt
     1201 gaaatctttg cccgacaggg tgtcgaactg agccgtgcat tactctccaa ctgggttgac
     1261 gcgtgctgcc agttaatgac gccgctgaat gatgccctgt accgttatgt gatgaatacc
     1321 cgcaagcttc acactgacga cacaccggta aaggtactgg caccgggcct gaaaaagacg
     1381 aaaacagggc gcatctggac gtatgtccgg gatgatcgca atgcgggttc gtcatctcct
     1441 ccggcggtct ggttcgcgta ctcaccgaac cggcagggga aacacccgga gcaacacctc
     1501 cgccccttcc ggggtatcct gcaggcggat gcgttcacag gttatgacag gctgttcagt
     1561 gcagaacgtg aaggtggtgc gctgacagaa gttgcgtgcc gggcccatgc ccggagaaaa
     1621 atccacgatg tatacatcag cagcaaaagt gcgacggcag aagaagccct gaagcgaatc
     1681 agtgaactgt acgccatcga ggatgaaata cggggattac cagagtcaga gcgtcttgca
     1741 gtcagacagc agcgaagcaa agcgttactg acgtcgctgc atgaatggat gatggagaag
     1801 aatggcacgc tgtcgaaaaa atccagactg ggcgaagcgt tcagctatgt actgaatcag
     1861 tgggacgccc tctgttatta cagtgatgac ggactggcgg agacggacaa taacacagcg
     1921 gaaagagcgc ttcgtgcagt ctgtcttgga aagaaaaatt acgtgttctt cggtagcgat
     1981 cacggcggcg agcgtggtgc actgctatac gggctgatcg gcacctgccg tctgaacggt
     2041 atcgatccgg aagcgtatct gcgccatatt ctgagcgtac tgccggaatg gccctccaac
     2101 cgggttgatg aactcctgcc atggaacgta gtactcacca ataaataagc gtcaatacgg
     2161 tgctccgttg acgcttacct tctttcattc gctgaaggtg gaatgtatcc acggggaacg
     2221 ctttagcagc cgggaaataa tgcgggaaac ggtgtttaat tatatcgagt gtgattacaa
     2281 tcgctggcgt cgtcacagtg cctgtggcgg tctcagcccg gactgtcttg cactgcaaat
     2341 attcagaaat gagtctatgc agttcaccag taaaatcagc catcagagag ggaatgtagt
     2401 ggcacagtaa atttggccac ctgattaaag gtgatatcct caccacaaca caaaacaggt
     2461 gacttaatga acaagaaaac caaacgtact ttcacccctg aattcaggct ggaatgtgca
     2521 cagctaattg ttgatgaggg ctactcatat cgacaagcca gtgaagcgat gaatgtcggt
     2581 tctaccacgc ttgagagttg ggtgcgccag ctcaggcgag agcgtcaggg gattgcgccc
     2641 tctgccacac ctattactcc agaccagcaa cgtatccgcg aactggaaaa gcaggttcgc
     2701 cgcctggagg aacacaatac gatattaaaa aaggcttctc accgatggtc atcgcaacac
     2761 gacgccagcc aatcatggtg ccaatgccga gcgccagtgc tactgccatg atgatccaca
     2821 ccggagcgta ctcaatggtg cttaacatat cgctgcgcag ttttttcagc aggttctggt
     2881 cttctttact gacgcctggc agtttcgcca gcttcgcgga agtatcagag atgcacagca
     2941 taatgcggcg cagctggctg cgctgactca cgcttaacgg ctcgtaactt tccatattgc
     3001 ctggcagcat cgttttaacg cgcgcaatcg catcaaaggt atttcccgga tgacagtgaa
     3061 actccgtcac ttgcgcgcca tcagtagatg ccgcaggcaa tggaggttcc atcgcaatca
     3121 acttctgcgg cagttcagga tgctgttgca ggtagtgttc gaagttggta acggcatcgc
     3181 gggtacgggt aatttcatag ccggacgcgt tcatattgac gacgaagcca gcaggggcaa
     3241 tccccaccag taccagcatc accaggccga tccctttttg tccgtcgttc gcgccgtgcg
     3301 aaaacgccac gcccgcagcg gaaacaatca gcgcaatacg cgtccagaat ggcggcttac
     3361 gtttgccttt tttctttttg cgatcttccg gaatgcggtg aatacggtca cgctttttcg
     3421 ttccgctcca gtagcgtcgc agcaggaata tcaggcctcc cgcaatgacc aggccgacga
     3481 taggggaaac aatcagcgag gagaaaattt tggtcacttc acgcaggttt aacgcatcca
     3541 tcaccgatga gccggttaac agcgcgttgg ttaaaccgat gccgataatc gcgccaatca
     3601 aggtatgcga actggaggcc ggtaaaccaa agaaccacgt tcccaggttc cagataatcg
     3661 ccgccagcag catggaaaag accatcgcca ggccgtgggt tgaccccata ttcagcaaca
     3721 aatcggttgg caacatatgg acaatggcat aggcaacgct aagtccgccc aataacacgc
     3781 caaaaaagtt aaaaaatgcc gccatcacca cggcaagctg tggctgcatg gcacgagtat
     3841 aaataacggt tgccaccgca ttcgccgtgt catgaaaacc attgattgct tcgtagaaca
     3901 acacaaatgc cagagcaagt aataacaaaa gccctgtgta tatatcaagg ccaacaaata
     3961 aatttagcat atattagatt acgccatttt gaatttacga acggacgcat tatcagtgac
     4021 tttaacggtg tgggcaaagt gaaatatcat tttttaacga taaaaagagt gttatttctt
     4081 gtgtatcaga caataagcat tcattaaaaa tctttagaaa taattagaac acaggcattc
     4141 tgctggttaa tgtattcaca tatgaaatga ataaatattt gaacatataa atattgaata
     4201 ttttgattaa tccccgtact gactattctt cataatcagt acgggattgc tacaacatgt
     4261 attactcttt caccacaatt aacggttcaa tatcactctc ttttttaata accagtgatt
     4321 catcaccgcg caaacacgtc ccaccgtagt tgccgccaac ggtgaaggta catacctgaa
     4381 tgtatttacc gtccactttc ggcaaacacc acagttgctg atagatgttt ttctgctcgg
     4441 caaatttacc actggttttg tccagcacct cttcatgatg gctgacgagg tcgatgttgc
     4501 tgccacagcg accggcgatc ggtttcactg cgtaacctgt tttcaccaat tcatcattaa
     4561 cagtgaaatc ggtatccagc aggtaacgat ggtgcgggaa cagcgaccag aggatcggca
     4621 gaatcgcttt gttgccgggg atcaccgtcc acagcggctc aaagaccagc acttccgggc
     4681 gcagcaatac gtcgataaga cgcacttcat tttgcggatg accggtacgg atcggcaccg
     4741 cagcaaactc acggtcgcta acttcacgaa tctgatcaaa cgcggtttcc cacgcccagg
     4801 ttttccacac gcagttaacc agtcgccctt ccccatcaat cagttgcccg gcagcatccc
     4861 agcccagttc atccagcccg cgcaagatac gcgtttcaaa gcccgcctgg tgcagcgcct
     4921 gctccataaa ctgcgcgtga tagttttcct cgatatcttt gtcctgcatg atatggacaa
     4981 acggacgtgc acgactgtgt ttccaggcac cagccagttc gttaatcagc ccttccgccg
     5041 gattgaagcc gttgccttta tagccctgct ccgcccaacg ttcgaggatc aagcccgctt
     5101 cggtatgaca ggaggcggag tcggcgttgt actcgtaaac cttcagacca cgctcatcca
     5161 tgcagaaatc catacgaccg gtgatcatat ggtgacggcg acgctgccag gagagacgca
     5221 aacgtggcca gaggattttc ggaatgtcga acagcgccag caggttgtca tctttcagca
     5281 ccttgtcggt tgcgtgaaga tacatcaggt gcagctcgtt ggtggcttta atcagctcct
     5341 gctcggcgct ctcggtaatg gtgtagtaat gatacggatc ctgattgatc acctgaccgt
     5401 tggcctgcac ataggcgttt tgcagcggat ctttttcatc cagccattta ccgtcaaact
     5461 ggcctttgtt ttccagacgc gctccgctga ttttcagcag ctcgcctgca atttccggct
     5521 gcggtaagct gtattcagta tcttccgtct ggatcatcca gcccagaatg gtggtgtcat
     5581 caaaagtgtc tttaagggta tagcagccgt tttcgaccac catctccagt tcgcgcgtcc
     5641 actgttgccc ttgcggcaac ggggaatgaa ccacgttctg ttccgcaata cggactttgt
     5701 tgccatgtaa ttgggtaatg atggcgacat ggccagtgtc tttaaattca ccgcctttat
     5761 cccagataag aagcgcaccc gcgaccggcg cacgcggcga gccgttagga aatgcctgca
     5821 atggcaggat gttgtcatta accacttcac gcaggaagcg cagcgagaaa atctcccacg
     5881 ccatacccac gtcagtaaag accacaccgt agttcagaaa gagaaaacgg cgagcaaatt
     5941 caacgcattg ccatttgtgg cccatatatt cgtcgtcgat atagctacgg aatacggcgt
     6001 catcttcgta ttcctgcgga tcgagagaac tgtaatctga agagtagatt gctaccccac
     6061 ctggggcgta gcccaataat gtcccgaacg gggcatcctg gctggtcgtt cctttgctca
     6121 tcactttacc tttaacaata caacctaagc agttggcgta attgttgtgc tctgattacc
     6181 tgctcagcga cattaaccgg acagaggtca gactaatcat acacctcatc gcagcggctg
     6241 gcgcaaaggg ttaaaaaatt cacattctgt acagcaagtg acctgctaca ctgcttcaac
     6301 actaccactc agaaggcaac tcactatgac agacaatact tatcagcccg cgaaagtctg
     6361 gacgtgggat aaatccgctg gcggcgcgtt cgccaatatc aatcgcccgg tttctggtcc
     6421 gacgcatgaa aaaacgctgc cggtgggcaa acatccatta caactttatt cgctgggaac
     6481 gccgaacggt cagaaagtaa cgattatgct tgaggagctg ctcgcgctgg gcgttactgg
     6541 cgcagagtac gatgcctggc tgatacgcat aggcgatggt gatcaattct ccagcggctt
     6601 tgtcgaagtg aacccaaatt cgaagatccc ggcgctgcgc gatcataccc ataatccgcc
     6661 gatccgcgtg tttgagtcag gatcgattct gctttatctg gcggagaaat ttggctactt
     6721 cctgccgcag gatctggcaa aacggactga aacgatgaac tggctgttct ggttacaggg
     6781 cgcggcaccg ttcctcggcg gtggttttgg tcacttttac cattacgcgc cggtgaaaat
     6841 tgagtacgcc atcaatcgct ttaccatgga agccaagcgc ctgctcgacg tgctggataa
     6901 gcaactggcg cagcataagt ttgttgcggg cgatgagtac accattgcgg atatggcgat
     6961 ttggccgtgg tttggcaacg tggtgctcgg cggcgtgtat gatgccgcag agtttctgga
     7021 tgcgggaagt tataaacatg tgcaacgctg ggcgaaagaa gtgggcgaac gtccggcagt
     7081 gaagcgtggg cgtattgtta accgcaccaa cgggccgctg aacgagcagt tgcatgagcg
     7141 ccatgacgcc agtgattttg agacgaatac ggaagataag cgtcaggggt aaggattggt
     7201 gttcgtcgca gcaagccatc caggccggat aaggcgttca cgccgcatcc ggcaatcgag
     7261 cacaatgcct gatgcgacgc tgccgcgtct tatcagacct acgaaagcta atcatcacgc
     7321 gctggtaatg tcgtaatcca tttggcgcag tgcgtctaat gtggctttgg cttcatcttc
     7381 gtcgatgatg ctcatggcaa atccgacgtg taccagcacc cactggccca gcagatcggc
     7441 agggttacct tcacaaatca gggcgatatt cacgtcgcgc ttgataccac atacttcaac
     7501 ctgcgcaagc tggtgaatat cttcaccgac agccagcacc tggcctggaa cgccaataca
     7561 cattgttaac tccggttatt caacttcaat acttttgacg atcagcgaat cgccggtatc
     7621 gacacgcaac cgctcgccgt gacagagcgg acactgcgca tcgtgctgat gaacctccac
     7681 cacctggctg caatcccagc accaggcctg ggcgggttta tagacgatat gtaaatcgca
     7741 cccttgcgcc accgttccgt ggcagacaat ttcaaaacta aaacggacgg cgctctcctc
     7801 aacgcaggag agcgcgccaa tttccagcca cacggcggtg acgcgcttaa catcgtgctg
     7861 ctccgcctgc cgctggataa tttcaacggc gctctggcaa agcgacaact catgcatttt
     7921 cgccactcct gcgaccaaac agcagggcgc gacgccctgc gtgtggtaca tccggattcg
     7981 tgactggcag cgaaaggatc attcgtgcgc agtcatccgt caggcgttgg ccctcttcaa
     8041 tcgacatact gtgcgaaagc ggtgacatca gcgaacagga gaggtattgc gaaacaccgt
     8101 ccagttcgcc aacggtaaag gtcatggtgc catacggcag ttgcagacca attttttcac
     8161 tcactttgcg cagcggccag agttgatccg ggccggggaa gataactgca ctcagcatcc
     8221 acggggtgat cacacacccc gtccactgac cttcgaacag cgtaaaatca gaaacataca
     8281 ccggcattga aggatgcaga aaagagagag cgtgcatcga acgccgggca atttcttcaa
     8341 acgctgcctg tacttgcgcc ttcggggagg tctggaaacc tgctatctct tcagtcatga
     8401 atcgcctccc gtgggatggc ttccacgcca gattcacgca gcgcagccag aacctgctca
     8461 agcgcaggtt caatcattgc ttcaaccgtc ggcgttaagc cgatgtgtgg ctccagcgat
     8521 tccggaatta cgccgaccag ggtcagcttt ttcggaaact cgccggtgaa gcgcagggcc
     8581 gacaagacgt cggccaggcc aagctgatgc ggagagattt tgttggtaaa caacgccgga
     8641 acttcttcat cccgcaggat catcatcgtt cccggcgcgc tcttcttcga cacaatggca
     8701 tccgcaataa tcagatgatc gcgatttgcc atgtcgccaa gcagctccat tcccgccgtg
     8761 ccgccatcga ggatctcaac ataatccggc agaatgtatc gttgctctaa cgcttcgaca
     8821 atccgcacac cgatggcttc atcggtcagc aaaatattgc cgacccctaa gactaaaata
     8881 cgcattacag aaccttcact gaaaccactt cgttgccgtc agcatccact acgtgtaccg
     8941 cacaggccat gcacgggtcg aaggagtgaa tggtacgcac cacttccagc ggtttattcg
     9001 gatcagcaac cggtgtaccc accagcgact gctcgtaagg accgacgtca tcattgaagt
     9061 tacgcgggcc agagttccag gttgatggaa caactgcctg gtagttgctg atgataccgt
     9121 ctttgatcac catccagtga gagagcatac cgcgcggcgc ttcgaggaag ccaacacctt
     9181 tgaactcacc cgttgccgga atgttcggct tcacaaaggt ggtgtgatcg cctttgccga
     9241 tattggtgat cagtgcactg tattggtttt gcaggatatc ctgcaattca cagcagtgaa
     9301 cggtacgacc aataatacgg cccagcgtgg agtgcagctg tgccacttcc agcgtgttgc
     9361 cagtcagttt ctgataaatc gcaacgattt cattcagttt gttctgggta gattcgcgac
     9421 ctgccgccag tttcaccagc atattagcca gcggacccac ttctaccgtt ttgccgtaga
     9481 aagtcggtga tttcacccag gaatatttcc cgtcgtcaga ccaaccatca taagccggaa
     9541 tggtggtacc ttcccacggt gcctgcggcg cttcgtcttt ataccaggag tgcttcgcgc
     9601 tttcctgaat gcctttgatc aggtattcat cggaatgaga agtgatcgga cgatacgagg
     9661 acagatccgc attctcaatg tagccgcccg ggaacaggaa gctgccgttt ttactgtcgg
     9721 tcgggaattc cggcacgctc aggtagttca ccgcaccttt accgcgcgtc agccattccg
     9781 ggtagaacgc agcgataacc gcagtatcga ccttgtaaac ctgctcaaca aagtcactca
     9841 gcttgtcgat gaaagacttg atgtacatca ggcgctcaag gttcagcacg cccagaccgt
     9901 cgaggttgat tgggttagcg accccaccta ccgccaggtt ctgaatgtgc ggcgttttac
     9961 cgcccagcag cgccacgacg cggttagcgt cacgctggca ctccagcgcc tgcaggtagt
    10021 gcgctaccgc aatcaggttc acttccggcg gcagtttcat cgccgggtga ccccagtagc
    10081 cattggcgaa aatacccaac tgaccgctgg caaccagatc tttgatcttg ttctgaactt
    10141 tggtgaactc ttccggactg ttcaggtgcc aggtcgaaac acctttcagc atttcggagg
    10201 ctttggttgg gtcagcttgc agtgcagaag tgatgtccac ccagtccagc gccgaaagct
    10261 gatagaaatg aacgatatgg tcatgcgtgg tgtgcgcagc cagaatgatg ttacggatgt
    10321 attgcgcgtt aaccggaacg tcgatattca gcgcactttc tgccgcacga acggaagaca
    10381 gcgcgtgagt ggtagtacat acgccacaga tacgttgcac aatcatccat gcatcgcgcg
    10441 gatcgcggtt tttcacgatc tcttccatgc cgcgccacat ggtaccggaa gcccatgctt
    10501 tcgaaacgac gccattttcg atttcgcaat cgatgcgtaa atgcccctca atacgggtta
    10561 ccggatcaat agtaattctc tggctcatgc tttgctcgcc tcatgacgat tatgatcgtt
    10621 ttgttttaaa ggaggaagta tcggcagtag acgaatgagt acgatgtaag cgcaaatctc
    10681 aatagccaca aaaccaatag aaatcaacag ttcttcccag gtcgggaagt aggcgtaacc
    10741 gccgcccggg ttgaatgcca ccagcgaata ggtcagacgc caggttgcac aacctaacag
    10801 cgcgctcagt gctgacagga acagcatgcg ggaatcatta cgcagcttcg ccacacgcag
    10861 aacgaccagc gggaagagca tcagcaggac ttcaatccag aacatcacgg agtagaagtc
    10921 accggcaaac gctaacgaca gcttgtcgcg atagataagc tcgccaaagc gcagcacgat
    10981 gaaaatcgcc agcaacacac tgatggtgtt ggtcagcttg acgaataggc tcttttcatc
    11041 cggaccgttg ccacgcagac ccgcctgcac cagtgaacct tcaaagatga caatcgagaa
    11101 gcccatgatg aacgccgtca gcagcgagaa cagcggcaac atttcatagc tctgccacag
    11161 cggatgcacc ttgtagcccg ccgagatcat cagcgacccc attgaagact ggtgcatggt
    11221 cggcagcagc gcaccgagcg cgatgatgaa gaacatcacc ttgttcagac gctgtagcga
    11281 caccttccag cccagacgtt caaacagtgc cggagcaaac tccagagcca tcacgccgat
    11341 gtagatggtc atacagaccg ccgtctcgaa cagtaccgag ttcacgttga agtgacccgg
    11401 aatgtagaag tacggcaggt tccagtagcg acccacgtcg atagtgatcg acaagccacc
    11461 cagtgagtaa ccaaacagac tcgccaacag cgccggacgc accagcggat ggtattgccc
    11521 acggttaaag acgtataccg cccacgcaag cgcccagccg ccacaggcaa agccggtgcc
    11581 aatcagcagg tcaaacgcga tccacacgcc ccacgggaag ccgccgttca ggtcagagac
    11641 agagcccaga ccgaacacca gacgcttcac aatcaggagc atacagatga cgattaacgg
    11701 tccaaaaatc atgaccggtt tactgatgat tttgccgccc agcggttgtg gatcatgact
    11761 catgatcgtc tcctccgtcg tgatggtcgt ttttggtgtt gcgacgaacc agcacggtca
    11821 agcccgccag cacagccagt ggtagcatca tgcctttata cagggtgtgt tgaatatttt
    11881 cggaacgcgc accggtagaa agatcgtcca gtttcggcag gtcgagattt tcataaggca
    11941 cacccgtcag taccagaacc tgagtaccgc cgccctcttt ctcgccgtac agatgcggat
    12001 aatatttcgg caccgtatgc aggtaagtgt cgccagattt cagcgtctga cgcggatagt
    12061 ggtattcgct gccaggcttc agcgccagac gttttttcgc ctccgccatc agctcttcac
    12121 gcgtaccaaa aatcaccgcg cccgccgggc acacttctac gcagccaggc agaccgcctt
    12181 tatcgagacg ttccacacct ttctggttgc acagctcgca cttatgcagc gcaccaaacg
    12241 ggttgttgta gtcgtacttc ggcacgttgt acggacaggc gaccatgcag taacggcaac
    12301 cggtgcacac gtctttgtcg taatggacaa tgccggtttt cggatctttt ttcagtgcag
    12361 agaccgggca cacagagaca cagttcggat cgacgcagtg catacactgt ttcttaatgt
    12421 acgcgtagcc gttctcctcc tggtctttgt tgacccctgt gccgctggtc cacacttgaa
    12481 tgatgttatt ggtatacggc gacagtttgt cgttgttcga ccaggtctgt tccccttgcg
    12541 ggttacgttc agggaagttg atatcctgac acttggtgac gcaagcctgg cagcctacgc
    12601 acaaggtcga gtcgtacaac atccccagcg atcccggaat tggcgggcgg ttttcagcag
    12661 ccgcatgact gacagacggc agcgcgcccg tcagcaatgc cccgcaggag gctgctttaa
    12721 taaaattacg tctgttcacg gttattctcc ccgtgagtca gcgttatctt tcttttgctg
    12781 acgacccagt tcacgcaccg ccatcacgct gacaccggca accagcccaa ccacaccgcc
    12841 gagcaaacca atagcgcctg cagagacgtt gccgccctct ttagcgttaa catccggttt
    12901 ctgtgaacgc ggagtctgat tttcgacgtt ggcaagctga tggatgcctt tatggaagcc
    12961 gataccttct tcgttacagc catagcacgg gtgaccaatc gccaccggcc acacaccgcc
    13021 aacatcgcag aattgcagcg ttgagcagtt gccgtaagtt tctggccctt tacagccgag
    13081 gtggtacagg caccagcctt cgcggtggcc ttcatcaccg aactctttgg caaaacgacc
    13141 agcatcgaag tgcgggcgac gttcgcagtg ttcgtgaatc agacggccat aggcgaaggt
    13201 cggacggttt ttgtcatcca gtttcggcgg tttgccgtaa gtgatgatgt gcgcaacggt
    13261 cgcgaggaag ttgtgcgggt tcggcgggca gcccggaata ttgataacgg ttttgcctgg
    13321 cagaacttct tgcaagctga ctgcgccagt tgggttaact ccagctgcgg caacaccgcc
    13381 ccacgcagag caggaaccga tagcgataat ggctgctgcg ccttccgccg ctttgcggat
    13441 gtgatccaca atcggctcac cggcaaccat gcaataaata ccgttatctt ttaatgggat
    13501 ggaaccatcc accactaaca catactgccc tttgtacttc tcgagagcgt tatgtttgtt
    13561 ctcttcgacc tgatgaccga aggcggcgga aagcacttcg tgatactcca gagagatagt
    13621 ctccagtacg aggttttcta ccgttggatg cgttgcacga agcagagatt ccgtacaacc
    13681 ggtgcactcc tgcgcgccaa tccagataac tggcggacgc tgcgggttag taaccgattc
    13741 ggccatctct gcagcggctt tgctacttaa ccccatggtg gcggctaatg ctgcacaaag
    13801 cttcatgaaa tcacgacggt taatgccgtg agaatggatg agggtgttat ctccagtcat
    13861 ttatagttat tccgttgcga agacctggca tatattttgc ctcaatcgca aaatcaataa
    13921 tgcgatcgat gcgccattta ccacacattt attatggtta tcgatatcat gatactgcgg
    13981 cggggcgcta aaacgaaggg aagaagcatt agtgtaatta attaaagcaa gataatacgt
    14041 atgtttgatc aattttcgag gcgataaccg gccataaaaa agcgcaacgt agaacaggaa
    14101 ttattctcac gaaagaaatc cattaccatc tctttatcca gtccatagcg gcgcgtcaga
    14161 atacccgctt cccaggcgct aagctggcga tctccggtct tttcgtacat ccggtgtgag
    14221 tagcctaata caaaaccgcg tttataatcg gcacagaatt ttgtcacatt aaccgcactg
    14281 tcggcatgcg ttgcttttaa tcccgccatt aagcctttac caaaatggtt attcatcgtt
    14341 cacctcattc gctatatatc gtaatatata gcgatatttt aggcaaagaa cgacttttgc
    14401 gcacgctatt ttttcatgct tgtttaagac gtaatcccgt aagagataaa cctatcagga
    14461 gagatattta tcgaaccatt tcagtgtcct ttgccaggca agatcggcgg cagatttgtc
    14521 ataacggggc gtggaatcat tatggaatcc gtgattaacc cccggataga tatacgcctc
    14581 ataaacctta ttattggctt tcaacgccgc ctcgtaagca ggccagccct cgttgattcg
    14641 ggtgtccagt tccgcgaagt ggagtagtaa aggcgcttca atcttcgcca catcggcagt
    14701 gggtgcctga cgaccataaa acggcactgc gcaggccagt tccgggtaag cgaccgctgc
    14761 cgcgttcgat acgccaccgc catagcaaaa tccggtaata cccactttgc ctgtcgcttg
    14821 cggatagcgt tgcataaact caattgcggc aaagaaatca ttcatcagtt tggttggatc
    14881 aacctgctgt tgcagctcac gacctttatc atcatttccc ggataacctc caacggaatt
    14941 taagccgtca ggtgccaggg cgatataccc cgccttcgcc actcgccgtg ccacatcttc
    15001 gatatacgga ttcagtccac gattctcatg caccaccacc acggctggcg ttttgccgct
    15061 catttttgcg ggtttaacca gataaccccg tacctcgccg tgaccatttg gcgaaggata
    15121 cgtgatgtac tcagcaaaaa tctccgggtc ggtaaactct acctgagtcg ccagcgcata
    15181 atttggcttg agcaaatcaa acaacgctaa tgccgtcatc ccgcctaccg catacttcgc
    15241 cgcaaggttg aggaactcac gtttcgagat tttcccgtga gcgtaatagt cgtagtaatc
    15301 cagcaactct tgtgggaaat ctttggcggt taaacgcggc atcgttgcac tcctcagttg
    15361 gtgttttttt aagcaaagca taagcacgtg tttttgccca gtttttcgtc actctgtgag
    15421 ccagactacg ggatacgcgc tggcgaatcg ctaaactaga aacattgttt cgaaattgaa
    15481 cggtggaaag gagaggtcat ggtctggtta gcgaatcccg aacgttacgg gcagatgcaa
    15541 taccgctatt gcggaaaaag cggtttacgc ctgcccgcgt tatcgctcgg tttatggcac
    15601 aatttcggtc acgttaacgc gctggaatca cagcgtgcga tcctgcgtaa agcgtttgat
    15661 ttaggcatta cgcactttga tttagccaac aattacgggc cgcctccagg aagcgcagaa
    15721 gagaactttg gtcgcctgct gcgggaggat tttgccgctt atcgcgatga actgattatc
    15781 tctaccaaag ctggctacga tatgtggccc ggcccttacg gctctggcgg ttcacgtaaa
    15841 tacctgctcg ccagcctcga ccaaagcctg aagcgtatgg ggcttgagta tgtcgatatc
    15901 ttttactctc atcgcgtcga tgaaaatacg ccgatggaag aaaccgcctc tgcgctggct
    15961 catgcggtac aaagcggtaa ggcgctgtat gtcgggatct cctcttactc accagagcgg
    16021 acgcaaaaaa tggtcgagtt gctgcacgag tggaaaattc cgctgttaat tcatcaacct
    16081 tcgtacaatt tactaaaccg ctgggtggat aaaagcggcc tgctggatac cctgcaaaat
    16141 aacggcgtgg gctgcattgc ctttactcct ctggctcagg gattgctgac cggaaaatat
    16201 ctcaacggca ttcctgaaga ttcacggatg catcgtgaag ggaataaagt tcgtggtctg
    16261 acgccgaaaa tgctcaccga agccaacctc aacagcctac gcttattgaa tgaaatggca
    16321 cagcagcgtg gacaatcaat ggcgcaaatg gcgttaagct ggttgctgaa agatgagcga
    16381 gtgacgtcgg tattggttgg tgccagccgc gcggagcaac ttgaggagaa cgtgcaggcg
    16441 ctgaataatc tgacatttag caccgaggag ctggcgcaga tcgatcagca tatcgccgat
    16501 ggcgagctga atctgtggca ggcgtcttcc gataaatgac ctgttaataa cgggcgcgag
    16561 aaccgcgccc gaatcagatc agtgattatg acgagtcagt cggtcaagat agcccattac
    16621 aaatgcagag agcacaaacg tcagatggat aatgacgtac cacatcagtt tgttatcagg
    16681 gacatttttc gcatccataa agacgcgcag taagtgaatg gaagaaattg ccacaatcga
    16741 cgctgctact ttgtttttca gcgacgttgc atccattttc cccagccagt tcagcttctc
    16801 tttgttctcg gagatatcga gttgtgagac gaaattctca taaccggaaa acatcaccat
    16861 caccagcaaa ccgccaacca gcgtcatatc caccagcgac agcaacacga ggatcaaatc
    16921 tgattccgcc atcgagaaga tattcggcag tacgtgaata atctcctgga agaacttcag
    16981 cgccagggca actaacgcca gcgaaaggcc aaagtacacg ggggcaagca gccagcgaga
    17041 agcatacatt gcattttcaa gaaaacgttc catacattcc tgtcgtgtag ttaaaacggg
    17101 caacagtata tcgcaattga gtaaacagcg cgcaacagca aatcaacata tccgttaaat
    17161 ctttgtcgca taattctgag ggttagggat cttcctggtt cccttatcaa cgcatcgaga
    17221 gcgtctaccc ttaaaggacg ttgaacctga agggagaaaa acgatgtctc atttaaaaga
    17281 cccgaccacg cagtattaca ctggtgaata tcccaaacag aaacaaccga cgccaggcat
    17341 ccaggcgaag atgacaccgg taccggattg cggcgagaaa acctatgttg gtagcggtcg
    17401 cctgaaagat cgtaaagccc tggtgacagg gggcgattcc ggaatcggtc gcgctgccgc
    17461 catcgcttac gcgcgtgaag gggctgacgt ggcgatcagt tatcttcccg tggaagaaga
    17521 agacgctcag gatgtgaaaa agatcattga agaatgcgga cgcaaagccg ttctgctgcc
    17581 aggcgattta agcgatgaga aatttgcccg ttcgctggtt cacgaagcgc ataaggcctt
    17641 aggtgggctg gatattatgg cgctggtcgc cgggaaacag gttgccattc ctgatattgc
    17701 agacctcacc agcgaacagt ttcaaaagac ctttgccatt aacgttttcg cgctgttctg
    17761 gctaacccag gaagcgatcc ccctgctacc gaaaggtgca agtattatca ccacttcgtc
    17821 aatccaggca taccagccaa gcccgcattt actggactat gcagctacga aggcggcgat
    17881 tctgaactac agccgaggcc tggcaaaaca ggtcgcggag aaaggtattc gggtgaatat
    17941 tgtcgcgcca ggcccgatct ggacagcact gcaaatttcc ggcggacaaa cgcaggataa
    18001 gatcccgcag tttggtcagc aaacgccgat gaaacgtgcg gggcaaccgg cggaactggc
    18061 ccctgtatat gtttatctgg caagtcagga gtcgagctac gtcaccgcag aagtgcacgg
    18121 cgtgtgcggc ggcgagcatt taggttaaaa aaatgcccgg ttgtgaaaag caaccgggca
    18181 tcattgtgaa ttcactctta cctgatgtgg cgaaaacgcc ttatccgcct acaaatgcat
    18241 gaagattcaa catgttgcaa tagattttgt aggcctgata agcgtagcgc atcaggcatt
    18301 gagcacctaa tgccggatgc ggcgtgaacg ccttatccgg cctacaaacg cacgaagatt
    18361 caacatgttg caatagattt tgtaggcctg atgaacgtag cgcatcaggc aattctactt
    18421 tacttcgctt tggcggtttc ttcgccgacc agacctatct tcaggtaacc cgcctgatgc
    18481 agcgtgtcca ttaccttcat caacgtctcg taatcgacag ttttatccgc tcggaagaag
    18541 atggtggtgt ctttcttgcc ttcggttaac gcattcaacg ccgtaatcat tgtttcatcg
    18601 gtgaccggat cgttaccgat aaacatcgag ttgtctgcct tcaccgacag ataaaccggt
    18661 ttttccgggc gcggctgcgg cgtgctggta gaagcaggca agttaacctt cacatctacc
    18721 gtcgctaacg gtgccgccac cataaagata atcagcagga ccaacatcac gtcgataaac
    18781 ggcgtcacgt tgatatcatg catttcgccg ttatcgtcga ggttttcgtt aagatgcatt
    18841 gccatcggac attatcctgc gcgtaatttt tgtgcgacac gaaccggatg cgcagcggcg
    18901 ctggcttcca gatccaggtc acggctttgc agcaacaata cctgcgctgc gacatcaccc
    18961 agcatcgctt taaagccgcc aatctgacgc gcgaatacgt tatagataac gaccgccgga
    19021 atcgctgcca ccagaccgat tgccgttgct aacagagctt ctgcgatacc cggcgcaacg
    19081 actgccaggt tagtggtctg cgtttgcgcg ataccaataa agctgttcat gatgccccag
    19141 accgtaccaa acagcccaac gaacggcgaa atcgcgccga tggttgccag atagccgtta
    19201 ccgcggccca tttgacgacc tactgcggcg acccgacgct ccagacggaa gctggtacgt
    19261 tctttaatac cttcgttatc gtcgctgcct tctgacagtt ccagctcatt ctgcgcttca
    19321 ttgagcaaat gcaggcttaa gcttttgcta ccaaaatcag cggcgatatc gttggcctgg
    19381 tttaaggaac gcgcttcagc cagcagttgc tgctcgcgct taaggcgacg cttctgatta
    19441 aagaactcta cgctcttact gaagaagatt gcccaggtga ctacggaggc caaaataagc
    19501 ccaatcatca cgcacttaac gacaatatcg gcgtgctgat acatacccca gacggaaagg
    19561 tccgtctgca ttaaattatt acccactgtg tatctccagg acgcaagtca caaaatctgc
    19621 gcataataat atcaaaacga cgtcgaattg atagtcgttc tcattactat ttgcatactg
    19681 ccgtaccttt gctttctttt ccttgcgttt acgcagtaaa aaagtcacca gcacgccatt
    19741 tgcgaaaatt ttctgcttta tgccaattct tcaggatgcg cccgcgaata ttcatgctag
    19801 tttagacatc cagacgtata aaaacaggaa tcccgacatg gcggacaaaa agcttgatac
    19861 tcaactggtg aatgcaggac gcagcaaaaa atacactctc ggcgcggtaa atagcgtgat
    19921 tcagcgcgct tcttcgctgg tatttgagag tatggaagcc aaaaagcacg cgacgcgcaa
    19981 tcgcgccaat ggcgagttgt tctatggacg gcgcggaacg ttaacccatt tttccttaca
    20041 acaagcgatg tgtgaactgg aaggtggcgc aggctgcgcg ctatttccct gcggggcggc
    20101 agcggttgct aattcgattc ttgcttttgt cgaacagggc gatcatgtgt tgatgaccaa
    20161 caccgcctat gaaccgagtc aggatttctg tagcaaaatc ctcagcaaac tgggcgtaac
    20221 gacatcgtgg tttgatccgc tgattggtgc cgatatcgtt aagcatctgc agccaaacac
    20281 taaaatcgtg tttctggaat cgccgggttc catcactatg gaagtccacg acgttccggc
    20341 gattgttgcc gccgtgcgca gtgtggtgcc ggatgccatc attatgatcg acaacacctg
    20401 ggcagccggt gtgctgttta aggcgctgga ttttggcatc gatgtttcta ttcaagccgc
    20461 caccaaatat ctggttgggc attcagatgc gatgattggc actgccgtgt gcaatgcccg
    20521 ttgctgggag cagctacggg aaaatgccta tctgatgggc cagatggtcg atgccgatac
    20581 cgcctatata accagccgtg gcctgcgcac attaggtgtg cgtttgcgtc aacatcatga
    20641 aagcagtctg aaagtggccg aatggctggc agaacatccg caagttgcgc gagttaacca
    20701 ccctgccctg cctggcagta aaggacacga attctggaaa cgagacttta caggcagcag
    20761 cgggctattt tcctttgtgc ttaagaaaaa actcaatgat gaagagctgg cgaactatct
    20821 ggataacttc agtttattca gcatggccta ctcgtggggc gggtatgaat cgttgatcct
    20881 ggcaaatcaa ccagaacata tcgcagccat tcgcccacaa ggcgagatcg attttagcgg
    20941 gaccttgatt cgcctgcata ttggtctgga agatgtcgac gatctgattg ccgatctgga
    21001 cgccggtttt gcgcggattg tataacattg ccacttttgg acaattttgc agacatttta
    21061 ttgtgaaaag tcttaaattg ttgcgtccgg gatcaaggcg tcccggacga ttcaggagta
    21121 caataggcag ataaaggctt aaacgctgtt ccacaggaaa gtccatggct gttattcaag
    21181 atatcatcgc tgcgctctgg caacacgact ttgccgcgct ggcggatcct catattgtta
    21241 gcgttgttta ctttgtcatg tttgccacgc tgtttttaga aaacggcctg ctgcccgcct
    21301 catttttgcc aggcgacagc ttgttgatat tggctggcgc attgattgcc cagggggtta
    21361 tggattttct gcctacgatt gcgattctga ccgccgcagc aagtctgggc tgctggctaa
    21421 gttatattca ggggcgctgg ttagggaata ccaaaacggt gaaaggctgg ctggcacagc
    21481 ttcctgctaa atatcaccag cgcgccacct gcatgtttga ccgccacggt ctgctggcgc
    21541 tgctggctgg acgttttctt gcatttgtcc gtacgctgct gccaaccatg gcgggaattt
    21601 ccggtctgcc aaaccgccgt ttccagtttt tcaactggtt aagcggattg ctgtgggtca
    21661 gcgtggtaac cagtttcggc tatgccttaa gtatgattcc gttcgttaaa cgccatgaag
    21721 atcaggtaat gacgttcctg atgatcctgc caattgcctt gttaaccgct ggcttgttag
    21781 gcacgctgtt tgtggtgatt aaaaaaaaat actgtaacgc ctgacgattt tccctgttcc
    21841 cggttgctgt accgggaacg tatttaattc ccctgcatcg cccgcattct tgccgcatct
    21901 tcccccggcg tcacaccgaa gtaacgttta aactcacggc taaattgcga tgcgctttca
    21961 tagccgacgc gcatcgctgc tgcgctggcc ttcatgccgt catggatgat catcatccgc
    22021 gccttatgca gacggtaatt cttcaaatac tgcaacggcg aggtgctggt gacagactta
    22081 aaattatggt ggaacgccga tacgctcata ttagcttctg ccgccagttg ctcgacgctc
    22141 aggttttcgg tgtatttatt ctcaatccgt ttcagcacgc ggctaatcag actgaagtga
    22201 gtctggcgac tgaccagcgc cagtaacgcg ccgccgcaag gtccggtcag cacgtagtac
    22261 agaatttcgc ggatgatctg tttgccgaga atacgcgcat ccagtggtcg cgccatcacg
    22321 tcgagtaacc gctccgccgc gcataaaatc tcttctgata acgtggcgga gttaatcccg
    22381 ctggctgcca tcgacggctg gaaatgctca tcttcgccaa tatccatcaa cagttcctgt
    22441 aactgcaaaa tatcgacatt gagacgcaac cctgccagcg gcacctctga cgtggcatag
    22501 gtttcgcact caaacggcaa cggcaccgtc agcagcaggt attcattggc atcataacga
    22561 aacacgcgtt cattgatata accgatttta tgtccggaaa agagaattat gatgccaggc
    22621 tcgtacatca ccggtgtacg tgcgaaaggc gtctcgccat acaacaaacg cacatcgggc
    22681 aacagttctg acaaactatt ttctttattt ttcagtttat taactttatc cgccagcaag
    22741 cggcaaatct cttcacgttt catatcgcgt aatttcttag gaataatgcg gcaatttgat
    22801 tgtgcgcaat tttgtagcat ttctccagca ctctggagaa ataggcaaga cattggcaga
    22861 aatgagcatt gagagccagg gcgctggcga tcacaatgaa aaacatcagg cagatcgttc
    22921 tctgccctca tattggccca gcaaagggag caagtaatga acaactttaa tctgcacacc
    22981 ccaacccgca ttctgtttgg taaaagcgca atcgctggtt tacgcgaaca aattcctcac
    23041 gatgctcgcg tattgattac ctacggtggc ggcagcgtga aaaaaaccgg cgttctcgat
    23101 caagttctgg atgccctgaa aggcatggac gtgctggaat ttggcggtat tgagccaaac
    23161 ccggcttatg aaacgctgat gaacgccgtg aaactggttc gcgaacagaa agtgactttc
    23221 ctgctggggg ttggcggcgg ttctgtactg gacggcacca aatttatcgc cgcagcggct
    23281 aactatccgg aaaatatcga tccgtggcac attctgcaaa cgggcggtaa agagattaaa
    23341 agcgccatcc cgatgggctg tgtgctgacg ctgccagcaa ccggttcaga atccaacgcc
    23401 ggtgcggtga tctcccgtaa aaccacaggc gataagcagg cgttccattc agcccatgtt
    23461 cagccggtat ttgccgtgct cgatccggtt tatacctaca ccctgccgcc gcgtcaggtg
    23521 gctaacggcg tagtggacgc ctttgtacac accgtggaac agtatgttac caaaccggtt
    23581 gatgccaaaa ttcaggaccg tttcgcagaa ggcattttgc tgacgctgat cgaagatggt
    23641 ccgaaagccc tgaaagagcc agaaaactac gatgtgcgcg ccaacgtcat gtgggcggcg
    23701 acgcaggcgc tgaacggttt gattggcgct ggcgtaccgc aggactgggc aacgcatatg
    23761 ctgggccacg aactgactgc gatgcacggt ctggatcacg cgcaaacact ggctatcgtc
    23821 ctgcctgcac tgtggaatga aaaacgcgat accaagcgcg ctaagctgct gcaatatgct
    23881 gaacgcgtct ggaacatcac tgaaggttcc gacgatgagc gtattgacgc cgcgattgcc
    23941 gcaacccgca atttctttga gcaattaggc gtgcctaccc acctctccga ctacggtctg
    24001 gacggcagct ccatcccggc tttgctgaaa aaactggaag agcacggcat gacccaactg
    24061 ggcgaaaatc atgacattac gctggatgtc agccgccgta tatacgaagc cgcccgctaa
    24121 gctttttacg cctcaaactt tcgttttcgg gcatttcgtc cagacttaag ttcacaacac
    24181 ctcaccggag cctgctccgg tgagttcata taaaggagga acgtatggct aatccaaccg
    24241 ttattaagct acaggatggc aatgtcatgc cccagttggg actgggcgtc tggcaagcaa
    24301 gtaatgagga agtaatcacc gccattcaaa aagcgttaga agtgggttat cgctcgtttg
    24361 ataccgccgc ggcctataag aatgaagaag gtgtcggcaa agccctgaaa aatgcctcag
    24421 tcaacagaga agaactgttc atcaccacta agctgtggaa cgacgaccac aatcgccccc
    24481 gcgaagccct gctcgacagc ctgaaaaaac tccagcttga ttatatcgac ctctacttaa
    24541 tgcactggcc cgttcccgct atcgaccatt atgtcgaagc atggaaaggc atgatcgaat
    24601 tgcaaaaaga gggattaatc aaaagcatcg gcgtgtgcaa cttccagatc catcacctgc
    24661 aacgcctgat tgatgaaact ggcgtgacgc ctgtgataaa ccagatcgaa cttcatccgc
    24721 tgatgcaaca acgccagcta cacgcctgga acgcgacaca caaaatccag accgaatcct
    24781 ggagcccatt agcgcaagga gggaaaggcg ttttcgatca gaaagtcatt cgcgatctgg
    24841 cagataaata cggcaaaacc ccggcgcaga ttgttatccg ctggcatctg gatagcggcc
    24901 tggtggtgat cccgaaatcg gtcacacctt cacgtattgc cgaaaacttt gatgtctggg
    24961 atttccgtct cgacaaagac gaactcggcg aaattgcaaa actcgatcag ggcaagcgtc
    25021 tcggtcccga tcctgaccag ttcggcggct aacgtgcaaa ttctcccggt ggcggtaatg
    25081 ttccgctacc ggacttttca gaaatcattt attcccctcg cgtcccgccc gttgttactc
    25141 ttccttgttc aggaatgcca aatataagga catcatcatg cagagccgga agctcttaaa
    25201 agaacaactc atctatatcc gggataaacg caacggagag gtgaaaaaca gatgaaaata
    25261 atacttctgt ttttagcagc cctggcaagt tttaccgtgc acgcacagcc cccctcacag
    25321 accgtagaac aaacagtccg gcatatttat cagaactata aatcagatgc cactgcccct
    25381 tattttggtg aaaccggaga gcgtgcgata acttctgtgc gtattcaaca ggcgcttacc
    25441 ctgaacgaca atcttacgct gccgggtaat attggctgga ttatgatccg gtttgtgatt
    25501 gtcaggattt tggcgatctg gtgctagaaa gcgtagcgat aaccgaaact gacgccgatc
    25561 atgccgatgc cgttgtgcgc tttcgtatct ttaaagatga taaagaaaag accacgcaga
    25621 cactgaaaat ggtggcggaa aatggtcgtt gggtcattga cgatattgtc agcaatcatg
    25681 gcagcgtctt acaagcagtt aatagcgaga atgaaaaaac gctggccgct ttagcttcgt
    25741 tgcaaaaaga acagccggaa gcctttgttg ccgaactctt tgaacatatt gccgattata
    25801 gctggccgtg gacgtgggtg gtttccgact cttaccgcca ggcggtcaat gccttctata
    25861 aaaccacctt caagacggcc aacaatcccg atgaagatat gcaaatagaa cggcaattta
    25921 tttacgacaa tccgatctgt tttggcgaag agtcgctatt ttcacgcgtt gatgaaattc
    25981 gagtcctgga gaaaaccgcc gattccgccc gcattcatat tcgttttacg ctgaccaatg
    26041 gcaacaacga agagcaagaa ctggttttac aacggcgcga aggtaagtgg gaaatcgctg
    26101 attttatccg cccgaacagc ggcagcctac ttaagcagat tgaggcaaaa accgccgcca
    26161 gattaaagca atgagctgaa ttaaatagca attagccgga acaataaaaa agggaacact
    26221 atatgaaaac gattttcacc gtgggagctg ttgttctggc aacctgcgtg ctcagtggct
    26281 gcgtcaatga gcaaaaggtc aatcagctgg cgagcaatgt gcaaacatta aatgccaaaa
    26341 tcgcccggct tgagcaggat atgaaagcac tacgcccaca aatctatgct gccaaatccg
    26401 aagctaacag agccaatacg cgtcttgatg cacaggacta ttttgattgc ctacgctgct
    26461 tgcgtatgta cgcagaatga taaaaaaatc cccggcagca tgtcagttgc cggggatttt
    26521 ttttaacgtc caaccgccgc tttagggcgt ttcttcgcac cagcattcac cggacgagat
    26581 tgcgtagacg acgctttttt tgccgtagca ggcgtctgac actgggtcgc catcggcgta
    26641 tgtttcgtca acgccggacg ggtattgcgg ttctggcgac gagcctcacg catctcttca
    26701 atggttggcg caggcactaa gcaatcgcga cggctgccaa tcagatgctt tttgcccatc
    26761 gcttccagcg cctggcggat taacggccag tttgccggat cgtggtaacg caacaacgct
    26821 ttatgcaaac gacgctgttt gtcgcccttc ggtacgaaga cgtcttcact cttataacca
    26881 atcttcgcca gcgggttttt cccggtgtaa tacatggtgg tcgagttagc cagtggcgat
    26941 gggtagaagt tctgtacctg gtcgagacgg aaacgatgct ttttcagcca cagcgccaaa
    27001 ttcaccatat cttcatcacg cgtaccgggg tgcgcggaga tgaaataggg gatcagatac
    27061 tgctctttac ctgcctgttt cgagtaggta tcgaacagct ctttaaagcg gtcataactg
    27121 cccatgcccg gcttcatcat cttcgacaac ggcccttctt cggtatgttc cggagcaatc
    27181 ttcagataac cgccgacgtg atgggtcgcc agttctttga tatagcgcgg atcttccacg
    27241 gcgatgtcat aacgtacgcc tgaggcaatc agaatctttt taatgccttt cagatcgcga
    27301 gcgcggcgat agaggttgat cgtcggttcg tggttagtgt ccatatgcgg acaaatatcc
    27361 gggtaaacgc aggacaaacg acggcaggtt tgttcagcgc gtggcgattt gcagcgcaac
    27421 atatacatgt tggcagttgg cccaccgaga tcggaaatta cgcccgtaaa acctggaacg
    27481 gtgtcgcgga tcgcttcgat ctcattaatg atcgaatctt cggaacggct ctgaataatg
    27541 cgcccttcgt gctcggtgat agaacagaaa gagcagccgc caaagcagcc gcgcataatg
    27601 ttgaccgaaa aacggatcat ttcgtaagcc ggaatacggg cattgccata ggccggatgt
    27661 ggcacgcgct tgtacggcag cgcaaaaacg ctgtccatct cttcggtaga aagcgggata
    27721 gcaggcgggt tgatccacac atagcggtca ccgtgttttt gcatcaatgc gcgggcgcaa
    27781 cctgggttag tttcgtggtg cagaatacgc gaagcatggg cgtacagcac tttatcgccc
    27841 ttcactttct cgaaagaagg cagcaacacg taggtttttt cccacggttt cgggcgcggt
    27901 ggctgcacgg ttacggcttt ggcttcctgc tttttcggtg ccaccggttt gttatccgcg
    27961 cacggcaaat cttcaccata cggatgcggg attgggtcga tttttccagg ggtatcaaga
    28021 cgggtggaat ccacgccgct ccagccaggc agcgcctctt tcacgataat cgcggtatta
    28081 cgcacatcgc ggatttcact aatcggctcg cccatcgcca gacggtgcgc cacctccacc
    28141 agcggacgct caccgttacc aaacatcagc atgtcggctt tcgaatccac cagcacggaa
    28201 cggcgcacgg tatcggacca gtaatcataa tgcgcggtac ggcgcagact agcctcaata
    28261 ccaccgagga tcaccggtac atctttccac gcctctttac aacgctgggt ataaaccagt
    28321 gtggcgcgat ccgggcgctt acccgcgacg ttatccggcg tgtaggcatc gtcatgacgt
    28381 aaacggcgat cggcggtata acggttgatc atcgaatcca tgttgccagc agtaacaccg
    28441 aaaaacagat tcggtttacc cagacgcatg aaatcgtctt tgctgctcca gtctggctgg
    28501 gcgatgatcc cgacgcgaaa gccctgtgct tccagcatac gaccgcaaat cgccatcccg
    28561 aagcttgggt gatcgacata cgcgtcgcca gtaaccaaaa tgatgtcgca gctatcccag
    28621 ccaagttgat ccatctcttc acgagacatc ggcaaaaacg gtgccggtcc aaaacaggcg
    28681 gcccagtact gcggccagga gaacaggtcg cgatccggtt ggatcaggga gatagagctc
    28741 attttgcttc cagaaatgat aaaaaaataa tcaaaggccg gggattataa gccggaacga
    28801 aagagaaatc gaaaagtatt ccatactcgc cctcctcggg cgagtatgaa gattacggta
    28861 ccggattgac taacagttgc ccaatcgagc cacggtccgc catttccagc gtctgactgt
    28921 tgaagtagaa cgggaagtgc gcccaggaag gctgaccgaa ataaacaagc agctccactt
    28981 gtccgtcaac ccaaacggta tctttccagc ctctgtcttc cgggaacggc atcgcaccat
    29041 tcacgttacg gatctggaac atcacgcctt caatatggaa cgcttgcggc tcgtccgcgc
    29101 gtaccgtcca gcgttcccac gttccttgct gcgcggtgac atcaatacgg ttgacgtccc
    29161 acagttgtcc attaataccc gggtcatcac ccagactgat atcgcgactg cgaatcggcg
    29221 aaccggccat gatttcagtt ggcagcaagc gcatcggaag actgtctgtg accagcggca
    29281 gaaggccggt tgggcgcagc gttagcacca gggtagaaac cagaatgctg gatggctcaa
    29341 agaagccacg aatacgatca acaatgctcg ccgcttcgcc acaggtgatc gacacttcat
    29401 caccgttgct catatccacc agaatctcgc ggcgctcgcc cggtgccaac gaaagttgct
    29461 tcaccgatac aggagcaggc aggaatccct gatcgccaga aatcacatgt aacgggcgac
    29521 catcgctcat ctgtagttga tagcgacgag agttcgacgc gttcagcagt cgcaagcgca
    29581 cccagccacg cgagacttca acgtacgggc tttgtacacc gttaaccagc agcgtatcac
    29641 caacaaagcc gccgcttccc ggttcgttgt attctggcgt accaaagtta tccagccgtt
    29701 tatcctggat aatgaccgga aaatcatcca caccataatg gttggggata ggcagcgact
    29761 tgctgacttc atcttccacc agccacattc cggcaaggcc gttatagacc tgctgagccg
    29821 tgcggttggg agtattggcg tgataccaca gagtagctgc gttctggcga ataggcagta
    29881 cgggtgccca gtcagcgttt ggcgacatca tccgtgccgg accgcccatc agcgggcctg
    29941 gtacctgtag cccggccacc gtcattgaga cattttctgt caggcggttg ctgtaaataa
    30001 gcttaacatc gtcgcccttc cagacgcgga tagtcggccc caggtaacga ccattgattc
    30061 cccagaccga cgcgcgtgtc cctggcgtaa atgaccagtg cgcacgttgt acagtcataa
    30121 acagcggttg cccacggcga gattcaagca gcggcggaac gggtagcggt tgctgttgcc
    30181 cggctgcgct ggccttcagg ggaacagcgc ctgcacaaag tgcaatcccc gatgcctgaa
    30241 tgaactgacg ccgactgagt gacatatttg ctccatgtaa aactggctaa agtaataccg
    30301 tgcaggaaag tgtttcccgc aaaaatacag attgccgact taaacttttc cggcggcttc
    30361 gcgctctgcg acttctttat cgagctcggc gattttttgt tccattatcg aacgacaatg
    30421 ggcagccagc tcacgaacct gatctttgcc atactgactg acgtcaattg gcggcagcat
    30481 ttcgacaatc accagaccgt tgtgcagtcg attaagatta atcttattcg aagttgtgga
    30541 gacgcacacg ggaataatcg ggacgcccgc cgcaattgcc gcgtgaaatg ccccagtctt
    30601 gaacggtagc aggccgcgac cacggctgcg ggtaccttcc gggaacatcc agatggaaat
    30661 acggcgtttt ttgaaatgat tcactacttc cgcaatagtg ccgtgagctt tagtgcgatt
    30721 gtttctgtcg atcaataagt tgccggttaa ccagtacaac tgcccgaaga aggggatcca
    30781 cagcaagctc tttttaccta ccgtcaccgt cggcggttgc acaatgttcg atgctgtcac
    30841 catgtcatag ttgttctggt ggttagcgat atagatagca ttgccgtagc tttcagcatc
    30901 tgcaggtttg cggcactcaa ctttcaggcc gaacagcggc gcaagacgac caaacatatg
    30961 cccaaaggtg gccacatgtt tcgggttacg cgggctgaaa aggcagtaaa tggagccgaa
    31021 tacacagact aagatgctgt aaatcacggt aataataaga cgaaagatat atagcataac
    31081 accctctgaa ggtctgagag actggcattg tacgtcacct gaaatcaggt tagggcattg
    31141 ttaaaagccg ctctctggaa aagcgtattg ttaatcgcaa cgcatgaata aacaacggtt
    31201 tcaagggaat ttttatcgtt tccgggggtt gaatacgctg ccggatgacg acttaacgtt
    31261 tcatccggcg ttccttgcaa gcgggaggaa acagcgccct ccccggcata ttactcttcg
    31321 ctatcaccgc tgctggcacg gcgaggagag tcgatctcaa cacgatcgat acgctgcaaa
    31381 ccgcgcatca acgtaccgcg gcgtccacgt tcgccagtga ctttctgtag ctcttccgga
    31441 cgcagtttaa ttttgcgttt cccaacatga atggtcagcg tgctttgcgg cggcagaacg
    31501 tacagttgcg ccagaccatc ctcgccacgc gcggcttctg ccgatggaat gttgataatc
    31561 ttgttgcctt tgcccttcga cagctgcggc agatcactta ccgggaacat caacatacgg
    31621 cctgcctgag tgattgccag cagcatatcg gaagcatctt caattaccac cggcggcata
    31681 acatgggcat tttccggtaa ggtgatcaaa gccttacctg cacggttacg cgccaccaga
    31741 tcgttaaagg tgcagacgaa accgtaaccc gcatcggaag ccatcagcag tttctgatcg
    31801 tcgctttcca tcagcatatg gtcaacggtc gccccaggcg gcaacgttaa tttgccggtg
    31861 agcggttcgc cctgaccacg cgccgacggc agcgtaatcg gatcaatggc atagctacga
    31921 ccggtggaat caacaaacac taccggttgg ttgctcttac ctttcaccgc cgctttgaag
    31981 ctatcacccg ctttataatt caggcccggc gcgtcgatat catggccttt agcgctgcgt
    32041 acccagccca tctgcgacag cacaatggtg acaggttcag acggcagcat gtcgtgctcg
    32101 ctcatcgctt tcgcttcttc gcgttcctgc aacggcgaac gacgatcgtc accgtaggct
    32161 tgcgcgtctg cctgcagttc tttcttcagc aggttattca ttttacgctc ggaagccaaa
    32221 atgccctgca actggtcgcg ctctttttcc agctcactct gctcaccgcg aatcttcatc
    32281 tcttccagtt tggcaagatg acgcagtttc agttcgagga tcgcttccgc ctgggtttcc
    32341 gtaaggccaa accgcgacat cagcgccggt ttcggttcat cttcattacg aatgatctca
    32401 atcacttcgt cgatattgag aaacgccacc agcaaacctt cgaggatatg caggcgcttg
    32461 aggactttct ccagacgata gttcagtcgg cggcgcacgg tatcgcggcg gaacaccagc
    32521 cattcggaga gaatttccag caggtttttc accgccggac gaccatccag accgatcata
    32581 ttaaggttaa tgcgatagct cttttccaga tcggtggtag cgaagaggtg gttcatcacc
    32641 tgatccatat ccacgcggtt ggaacgcggc acaatcacca gacgggtcgg gttctcgtgg
    32701 tcagattcat cgcgcagatc gtcaaccatc ggcagctttt tgttgcgcat ttgcgcagca
    32761 atttgctcca gtacgcgcgc acctgaaacc tgatgcggca atgcgctgat aaccaccgcg
    32821 ccatcttctt tcttccacac cgcgcgcata cgcactgaac cacgtccgtt ctcgtagatt
    32881 ttacggatct cggcgcgcga agtgataatt tccgcttcag tcggataatc cggcccctgc
    32941 acgatatcca gcagctgatc gagcgtggtt ttcggctggt cgattaatgc gattgccgcc
    33001 tgagccactt cacgcaggtt atgcggtgga atatcggtcg ccatgccgac ggcaataccg
    33061 gtggtgccgt taagcaaaat gtttggcaga cgggcaggta gcattttcgg ctcctgcaaa
    33121 gtgccgtcga agtttggcac ccagtcagcc gtcccctgcc ccaactcgct caatagcagc
    33181 tcggaatatt tcgacaaccg ggattcggtg taacgcattg ccgcgaacga tttcggatcg
    33241 tccggcgcgc cccagttccc ctgaccatca accagcggat aacggtaaga gaacggctgc
    33301 gccatcagga ccatcgcttc ataacaggcg ctatcgccgt gcggatggta tttacccagt
    33361 acgtcaccga cggtacgggc cgatttttta aatttggcgc tggcattcag gcccagttca
    33421 gacatcgcat acacaatgcg gcgctgaaca ggtttcagac catcaccaat aaacggcaac
    33481 gcacggtcca tgatcacgta catggagtag tttaagtagg cgttttccgt aaattcatgt
    33541 agcgcaaggc gctctgccat atcgctcatt aattctgatt cctcaactta ttcgccagcc
    33601 ttcaaagggg caatattgcc ggagatacta cattatctgc cgcgtcgagt cacaggagat
    33661 cagcgtgaat agcgccgccg gacgtgctcc gacggcgata aaggcatcaa tgtgccttga
    33721 tatacaactc tttgctgtag acataatcct ggggattatg cagcggaaaa ccgccaacgt
    33781 agggtttaag cagtttgatt aacggctgat agtagatagg aatcagcggt gcctgctggt
    33841 tgacgatcac ttctgcctgc tgatacaacg cattacgctt tgtcgcatca gtgatctgca
    33901 tggcctggtt tagtaaggcg tcatactgcg catttttcca gtgaccgacg ttttcttcac
    33961 tatcgctttt gagcgtgttc aggaagctgg aagcatcatt gtacgtcgca tcccacgact
    34021 gccgagacag catgaaatca ccggctcgtc gggcatcaag ataggttttc cactccattg
    34081 tgcgcagcgt cacctgtgca cccagccatt ttttccattc ggaagacaac gctatcgcgg
    34141 tcttttcatg cagatcgtac ttgttgtaga acagctcaaa gcgtagcgga tgagaggcgt
    34201 cgtatcccgc ctgtttcagc aaggcttttg ccatcgcgac gcgctcactc attggctttt
    34261 gcagttcatc gaacgtcgtc gcgctaaagc cttttacctc tggcggcgtc agcgtggttg
    34321 cgggcgttct caaccccagt accttttgcg caataagctg tcgatcaacc gtaagatata
    34381 gcgcccgacg cactcgcaca tcgttaaatg gcggtttctc aaggttgaag ttgtaatatt
    34441 cgctgttcag acgcggaata attcgtatct cgccaggcag tgatttttca atggcgggaa
    34501 tttgctgcgc cggaacccag gtgagatcga cctctcccgc gcgatagcgg ttatagccgg
    34561 tgaccgaatt atccagcgcc agatactcaa cctgttgcaa tactgtatgt tgcgcatcgc
    34621 ggtactttgg atttttgcgt gcagtaatct tttcgttaac tacccactga tcaagcacaa
    34681 aggcaccgtt gtaaaccatg ttctctggct tactccagct atcgccatgt ttagcgatga
    34741 catgatgagg aaccgggaac agcgtcggcc aggcgagcat cgtcgtgaac caaggaaccg
    34801 gctgctcaag cgtaacttca agagtacgat catccgtcgc tttgacaccc agcgatgtaa
    34861 catctgcttt acccgcaaca atagccgcgg cattgttaat gtgcgcctgt gccagatagc
    34921 cagcaaaagg gcttgccgtt ttcgggtcaa ccgcgcgctg ccagccgagg acaaaatcct
    34981 ctgccgtcag aggctgaccg tcggaccact gcaaaccgct acgcagatgg aaaatatagc
    35041 gcctgccgcc gtccagtatc tcccagcgtt cagcctgagc gggctgcacc tggccttcac
    35101 cgtccatcca taccagacct tcaaacagat ccagcacaat ctgcgcggca gtattctcct
    35161 ccaccttttg cgggtcgagc gtacctgggt cgctatgatt gttgtaacga aagacttgtt
    35221 gcggggcgag ctgtgtgttg gtgggaacgt cagcagcgta gagaggagcc gcacttacca
    35281 ggctgaccag ccataataaa tttcgcgtat acatggtatt ccttaccgga gaaattataa
    35341 gcccaacaac aatacgcctg tggcattgtt cgctcaaact tatcgcgagt gatttggctc
    35401 acactccggt aaagattccc catcacgcta acggcacata aacattcgtt tccagtcgct
    35461 gctccgtcac cccttcggca agattggtgt agtgaaacag aatcggtgct ttacgcattt
    35521 tctcgccgct tgcaggcagc cagtggcgaa taatgcccca tatcgtgtgt gaaatatcgt
    35581 ccagctcgcc aacgtggcga gccacggcat aacgtccacc ggtaagttca ccattgctca
    35641 caccataacg attatcggga atcggttcgc taacgctgcc gcagatatca aagcgaaacg
    35701 cttccggtgc ggtggtggca gggtcgtccc aggcgacgcc aaaagtctgg ctttggttaa
    35761 caggagaaag ccccgtctct ttacgccaca tgataaattt cgccacgctg tagttgagca
    35821 actcaggcct acagcggtgc ggcaacatcg ccacagggat tgatgggaaa tcgataatgt
    35881 ttacatccag tgtcaggttt gtcatgttgc ctccctgcga aattattccc gggaacgcat
    35941 cccgggagcg gtaacaatta cggatttact ttgcggatct gtttgacgtc aatttcaaca
    36001 gaattccagt ctttatcgac ttcaccctga atctcaaccg tatctttcgg cgtcaccgtc
    36061 acgccgttcc agcgtttgtg gtcgatatca acattgatag taccgctggc atctttgaac
    36121 acgtagagat cgtcagagat gcgttcaacg atattgccgc gcagggttac ccaggtgtcg
    36181 tcacgcaggg attttgcgct ttctacagtc gttacgctgc cgttcggccc ctggaatcct
    36241 ccggcctgac tttgcgttgc cgatgggcca gaaaaaccgc cctgctctgc tgccatcacc
    36301 ggtgcgctgc acagggccat tactgcgatt actgctgcga attttttcat gtttattact
    36361 ccctttaatg tctgtttccg agcatttaac aagatagtcc ttaacaactt cttaagggaa
    36421 aaaaataaaa tttagtgctg tacagagcgc gttacaacac ggtttactag cagcaaatac
    36481 ggttatcgca gggatgaaaa aatgcgaatt ttactgatag aagatgacat gctgattggc
    36541 gacggcatca aaacgggcct tagtaaaatg ggttttagcg tcgactggtt tacacaaggt
    36601 cgtcagggaa aagaggcgct ttatagcgca ccttatgatg cggtgatcct ggatttaacc
    36661 ttaccaggca tggatggtcg cgatattttg cgcgaatggc gagaaaaagg tcagcgtgag
    36721 ccggtactga tcctgaccgc gcgcgatgcg ctggaggaac gtgtagaagg gctgcgtctg
    36781 ggagctgacg attatctgtg taaacctttt gcgttgatag aagtcgccgc caggctggaa
    36841 gctctgatgc gccgaaccaa cggccaggcc agcaacgagc tgcgccacgg taacgtcatg
    36901 ctcgaccccg gcaaacgtat cgccacgctg gctggcgaac ccttaacact gaaaccaaaa
    36961 gaatttgccc tgctggaatt actgatgcgt aacgctggtc gggtactgcc gcgcaaactg
    37021 attgaagaga aactgtatac ctgggacgaa gaggtcacca gtaatgccgt tgaagtgcat
    37081 gtgcatcatc tgcgacgcaa actcggtagt gattttattc gtaccgtgca tggtattggc
    37141 tacacattag gtgagaaatg aaatttaccc aacgtcttag tctgcgtgtc aggctgacgc
    37201 taatcttttt aattctggcc tcggtgacct ggctgctttc cagctttgtc gcctggaaac
    37261 aaacaacgga taacgtcgat gaattgttcg acacccaact gatgctgttt gccaagcggt
    37321 taagtacgct cgatctcaac gaaatcaacg cggcggatcg catggcacag acgccaaata
    37381 aattaaaaca cggtcatgtt gatgacgatg cgctgacctt tgccatcttt acccacgacg
    37441 gcagaatggt ccttaacgat ggcgataacg gagaagatat tccctatagc tatcaacggg
    37501 aaggttttgc tgacgggcaa ctggtgggcg ataaagatca atggcgtttt gtctggatga
    37561 cctcacctga tggcaaatat cgcatcgttg ttggtcagga atgggaatac cgtgaagaca
    37621 tggcgctggc gattgttgcc gggcaattga tcccgtggtt ggtcgcactg ccggttatgt
    37681 taatcatcat gatggtacta ctgggtcgtg aactcgcgcc gctgaacaaa ctggcgctgg
    37741 cactacgtat gcgtgaccct gactcggaaa aaccactaaa cgcgactggc gtacccagcg
    37801 aagtgcgacc actggttgag tcgctaaatc aactgttcgc ccgcacacat gcgatgatgg
    37861 ttcgtgaacg acgctttacc tccgacgcag ctcacgaact tcgtagcccg ttaacggcgc
    37921 tgaaagtgca aaccgaagtt gcgcagctct ctgacgatga tccgcaggcg cggaaaaaag
    37981 cactgctcca attacattcc gggatcgatc gcgctactcg tctggttgat caactgctca
    38041 cgctatcgcg gctggactca ctggataacc ttcaggacgt cgcggagatc ccgcttgaag
    38101 atctcctgca atcgtcggtg atggatattt accacacggc gcagcaggcg aaaattgacg
    38161 tgcgactgac actcaatgtc cagggcatca aacgcaccgg acaaccgcta ttgctaagtt
    38221 tgttggtgcg aaatttgctg gataacgccg tgcgttacag tccacagggc agcgtggtgg
    38281 acgtcacgct gaatgccgat aatttcatcg taagggataa cggccccggt gtgacaccag
    38341 aggcactggc gcgaattggc gaacgcttct atcgcccacc cggacaaacc gctaccggca
    38401 gtggacttgg gctatcgatt gtccagcgaa tcgccaagct gcatggcatg aatgttgagt
    38461 ttgggaatgc ggaacaaggt ggatttgagg cgaaggtaag ctggtaaggg taagactttt
    38521 gctaaattcg caaaagactt tgcacatttt gctaatttca ccgtaccgct ctgtgacgta
    38581 ctatagtcgg caaacgtctc accttgaggt taaaaaatga gcaacatcct gattatcaac
    38641 ggcgcgaaaa aattcgccca ctccaatggt caactgaacg acaccctgac cgaagtcgcg
    38701 gatggcacac tgcgcgacct cgggcatgat gttcgcatcg ttcgcgccga cagcgactac
    38761 gatgtcaaag cggaagtaca aaactttctc tgggctgatg tggtgatctg gcagatgcca
    38821 ggctggtgga tgggcgcgcc gtggacagtg aaaaaaatac attgatgatg tattcaccga
    38881 aggtcacggg actctgtatg ccagcgatgg tcgtacccgc aaagatccgt cgaaaaaata
    38941 cggttccggc ggcctggtac agggcaaaaa atatatgctt tctctgacct ggaacgcgcc
    39001 aatggaagcc ttcaccgaaa aagatcagtt cttccacggc gttggcgttg acggtgtgta
    39061 tctgccgttc cataaagcaa accaattcct cggtatggaa ccgctgccga catttatcgc
    39121 taatgacgtg ataaaaatgc ctgatgttcc ccgctatact gaagaatatc gcaagcatct
    39181 tgtggaaatt tttggttaac tagagctcag gctttagaag gagttaacca tgcttaccgt
    39241 aatcgcagaa atccgtactc gtcctggtca acatcaccgt caggcggtat tggatcagtt
    39301 tgctaaaatc gttccaaccg tactgaaaga agaaggttgc cacggctatg cgccaatggt
    39361 ggattgcgct gctggcgtga gtttccagtc tatggcaccg gattctatcg tgatgattga
    39421 gcagtgggaa agcatcgcgc atcttgaagc gcatctgcaa accccgcaca tgaaggcgta
    39481 tagcgaagcc gtaaaaggtg acgtgctgga gatgaatatt cgtattctgc agccagggat
    39541 ttaacattcc tgaaaagagg ccggtgttgc taccggcctc aggtcagatt agccattctt
    39601 tcttcccgtt ttcagccccc gatggaaatc gttaattcgc ttaatggttt taaccgcacg
    39661 ctgcggcacc gcggaagcca ccgaccagac aatcatttcc aggcacaaca ataccgtacc
    39721 atgtagtggc gttttcccct tttcatcccc gcgcggaaca tgaatcacga tactggcgtc
    39781 tttgctaaaa cgtgaatcca gcgcgtttgt cagcaaaata acagggatcc ccagccgtct
    39841 cgcttcacgc agcgttgtca gcccttcccg gtgcgcagat ttttgcgcca tcatgatcag
    39901 aacatcgcca cgttgaagcg caatcagttg ctcggcaagg ccaatacccg tacggtttag
    39961 tgccgtggca ggcaaaccta tgcggttgaa tagtcgtgcg gtatagtccg ccagaatacc
    40021 cgaagcgcca atgccaaaaa ttgcgacttg ccgtgcctgt accaacaacg ctaccgcttg
    40081 cgcaagcgcg tggcggtttt caggctctga caggacatta caggtataca aatgcccttc
    40141 aagaacaaag tcgattgcgg tgttaacatc gctcgtcaag ttactcaccg tcgtagacat
    40201 cttttcgctg gaactaaggg ctggaccaag ccactgttcc agagtgcgtt tcagatcacg
    40261 cagcccggca aaccccaggg cctgtatagc cctgataact gtggcatcgg aagtgttcaa
    40321 cgtggccgca atttccatcg ccgtttgatc gagtaccact tcacgatgct cattaatata
    40381 gctggcgaca gctcttaacc caggcgttaa ctgactcgcg cgagtgcgaa aacgctcacc
    40441 ataaatatca acccgcgttt tgtctttctg aatcatttgg cctccggtaa aggtcgcccc
    40501 gcaatttgtg attgtatctg cgcagccatc agccccagag atgcaaagga gttagaaatt
    40561 gcctcttcac gagaaatcag cacataacgc ccggaacgac aggcagtcag aaactgacac
    40621 catcccggca tgactttttc cattgctgcc agctcatcct gaggtttacc accggtgtca
    40681 ccgcgccagg ttgcaaaaac aaaatcggca tccagttcag gtaaacgctc cgcgctcaca
    40741 tccattcgcc caccttcagg aatgctctca atcagcggcg gaaagcgaaa accagcatcg
    40801 cgtaataccc gccccagcga atgatagcta tgcatcacat taatttttcc ctgatttgcc
    40861 tgaatcaccg ataccgttat tttttggctg tccagcgtcg ccttcagcgc attgatttgt
    40921 gcctgatagc ggcgttcaag tatcgccagc tgcgactgcg ttcctgtcag ttctgccagc
    40981 ttgcgataga tttctggcgc gccccctttg agatgatcaa tgctcaccgt gggcgcaatt
    41041 ttttccagtc gttcaatggg tgtattacgg gtcggttctg tgatgatcag gtcaggcttt
    41101 gccgccacaa tcgcttcaat atcaatatca gcggtgccaa taaaagcgat cgatgaatta
    41161 tcaaagtcga cacctgtcaa taacgcgcct gatcgaatga aatggctacc gtcaggccga
    41221 gttcgcccgt ggctggcgac aggaggtaca ccgagctcaa tcagaggaat agtgatatca
    41281 agatcgtgta aagagacaat tctctttgga tgagcaggaa cagtgacttt gcgccctaaa
    41341 tcatcagtaa agacctgaac aggttctgtc gcgcgggcaa agaatgacaa cagtatcagc
    41401 agagaaaaaa ataaacgcat ataactccca ttaaaaactg ttcctgcgct gccagagcag
    41461 tagcaaaaag aatggtccac caatcaatga aataatgatt ccggcaggca gttgcaaagg
    41521 caaaaaagcc aaacgaccaa tgctatccgc caggatgact aacaacgccc ccgatacggc
    41581 gctccccagc agtaatgtcg tctgcccgcc acgtagaata aaacgtgata tatggggtgc
    41641 tatcaatccg acaaaaccga tgttaccaac acacgaaacg cagacggctg tcaggattat
    41701 tggtgcggcg acacgtaaca acgccagacg agaactattc acccctaacc cggttgcaac
    41761 ctgatgacca agcagagcga tatccgccgt cctggcggtg aacagcaata aaagagcggc
    41821 tggcaacatc caacaggctg aaataccgac taacatccag ttagcagcat gcaggcttcc
    41881 cgataaccac atcaatgcag tttgtacatc tcgaacatct gcagtggtca taaatacacc
    41941 gataccggcg gcgaaaaacc aggaaacacc aataccaatc agaacaaatc gcgggcggga
    42001 aatttcgcgt gcacaaaaaa tcacgattaa agccaccaac aagccaccag caagaccagc
    42061 cactggacgc cagaacattc ccagcattgg aaactggaaa atcagccaca gcaccgccac
    42121 gctacatcct tctttgacgc cgattaaacc aggatcggca aggccgttac gcgcaatgga
    42181 ttgcatcgcg gcccccgcca tacctaacat ggctccgcat agcaatgcca tgatgactct
    42241 cggcaagcga atatcctgca caatataacg tgcgtctgtg cttacgtttt ctggagcaaa
    42301 caacgctcgt cctacttccg atgccgggac aaaaaaagaa ccatgcatca gaccgaatat
    42361 cagtattcca accgcaaaca gaaacagccc accaagcaac accagatttt tagggcgtac
    42421 cagtgtcgaa aacttaccaa cacgtagtgg acgtagcccg acgttcatta tccgactcat
    42481 ttgaacattc tcgtcgccat aatgacaaaa acaggcgcac caactaacgc cgtcatgaca
    42541 ccggtcgcta actcatgggg cgtgaaaagc gtccgagcaa taatatctgc cagtaataac
    42601 aaaagcgcgc cgacacaagc tgaaagaggg agcagaacgc gcaaatccgc tgataccaga
    42661 cgcctgacaa tctgcggtac tagcagaccg acaaaaccaa tggggccagc aatcgatacg
    42721 gctgcgccac agagcaacgc gatcgcaagc agagtgaatg ttcgcgtgcg gagtaccgag
    42781 acgcccagcc cctgcgccat gcgatcaccc agtgccaaca tgttaagaga gggcgcaagg
    42841 taaatggcta acacaacccc ccccagagaa aaccaggcgg acgtacccag cgtcgcccaa
    42901 tcctgccccg ctaaatcacc tgctagccat gtccgcatcg ccagcagtgt ttgttcgtca
    42961 agaatcagga ttgcagccgt tattgaggac acgaacgccg atagcgccac gccgcataaa
    43021 gtaactttca ttggcgttag ccctgaacgc cctgcggagg aaagcagcaa tatcaacagg
    43081 aacaataacg cgccgcccgt agatgccagc agtgggcgtc caaccggaaa ggccagccca
    43141 agcgcactgg cagcaacgac agcaagagcg gctccggcat ttaagcctaa aatatgtggc
    43201 tcccccaatg ggttacgaat gacagcctgc aacaaagcac cagcaactcc aagagaagca
    43261 ccggtaagca atgctgccgc taaacgcggc aaacggagtc ttacaattac gttgtgatcg
    43321 aagttttgcg gttcaaaatg aagaaatgcc tgcaacaccg tttgtggtgc aataaatcgg
    43381 gcaccaatcc ctggatatac cacgcatccc acaagcagta aggcgaggaa aattacagta
    43441 accagccctg tgcgcatcat actttttcta caccacgaaa cggcatgaag aacggctttc
    43501 ccgtcagagt atttattgat gcgtgaacat caacgtcaaa gacagctttt accagctcag
    43561 gcgtgcaatg ctccccctca ttcaacacac gcaccacttt cccctggcgt aaaaatatca
    43621 acgtatcacc ataattgacg gcaaaattca gatcatgcaa caccacgacg actgtacgcc
    43681 cgtgatgacg cgttaaatca tgtaataact caagaatttc aacctgataa cgtaaatcaa
    43741 gccaggttgt gggttcatcc agtaggatat aaggcgtctt ttgcgccaac accatcgcaa
    43801 tccagcaacg ctggcgctgc ccaccagaga gtttttcaac aggtaaatga gcaaactcct
    43861 gcgtaccggt taatttcagg gcctcttcta ccgcctgctc gtcggcgtcg ctccattgac
    43921 gtatgaaatt ttgccaggga aaacgcccac gcgacaccag ttcataaacg gttaagccct
    43981 ccggcaataa tggcgactgc ggcaagatcc caagtctacg ggataacgct ttcgttggtt
    44041 gctcgtgaat cgctttacca tccagcaata ttgagccgcc catgggttgc aagacacgtg
    44101 caatggtgct gagcaacgtc gactttccac agccgtttgc cccaaccaga acggtcattt
    44161 tttctgtggg aacggagaaa gaaacaccat caacaatgat tttcttgtga tacccggcag
    44221 aaacattgtc gaggatcagc ccctgccctt ttgcgttatg tgccacgtgt acgccaactt
    44281 aatcaaagaa aaacaaaaat aaaacttatt ctcttttgag agtttgacgc cacaaaatgt
    44341 agtagtcaag aagttaaa
//
LOCUS       DAZFAB010000028        42614 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZFAB010000028 DAZFAB010000000
VERSION     DAZFAB010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42614
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.028"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            493..942
                     /gene="alaE"
                     /locus_tag="ACHJWA_001816"
     CDS             493..942
                     /gene="alaE"
                     /locus_tag="ACHJWA_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFO4308432.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(979..1323)
                     /gene="ygaC"
                     /locus_tag="ACHJWA_001817"
     CDS             complement(979..1323)
                     /gene="ygaC"
                     /locus_tag="ACHJWA_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFO4308433.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            1475..1804
                     /gene="ygaM"
                     /locus_tag="ACHJWA_001818"
     CDS             1475..1804
                     /gene="ygaM"
                     /locus_tag="ACHJWA_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFO4308434.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            complement(1954..3288)
                     /locus_tag="ACHJWA_001819"
     CDS             complement(1954..3288)
                     /locus_tag="ACHJWA_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFO4308435.1"
                     /translation="MPRYQDIARQLKTVIEQGKLKPGARLPSSRTWSQELGVSRSTVE
                     NAYAELVAQGWLVRRGQAGTFVSEQIHPQQSAVDVVAFAGESQQPLPFQMGLPALDLF
                     PRELWARVMGRRLRTQTRFDLALGDVCGEAALREAIVDYLRVSRGIDCQPEQVFITHG
                     YAASMALILHALAQPGDGMWMEDPGFPLIRPIVTRHGVEILPVPVDDNGLDVTSGIQN
                     YPDARFALITPAHQSPLGVALSLARRYQILEWAERSQAWIIEDDYDSEFRYHGKPLPA
                     LKSLDAPQRVIYAGTFSKALFPALRCAWLVVPVEQISQFRQQASLEPCAVPVLWQNTL
                     ADFIREGHFWRHLKKMRQHYAQRRQWIEQALTQQGFQVVPQKGGIQMVIKLAGNDVTF
                     VHKANAAGLAVQALSDWRIRSSEDGGLLLSFTNIVSESMARQVAQQLRSALN"
     gene            3376..3807
                     /locus_tag="ACHJWA_001820"
     CDS             3376..3807
                     /locus_tag="ACHJWA_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFO4308436.1"
                     /translation="MTTLRQPYYELSPAVYNALVQAKTALENSTLDTTLMELIYLRVS
                     QINGCAFCLEMHSKALRKSGVPQHKLDALAGWRVSHHFDERERAALAWAESVTDIART
                     HAEDEVYQPLLEHFSAAEISDLTFAIGLMNCFNRLAVSMRM"
     gene            4016..4261
                     /gene="nrdH"
                     /locus_tag="ACHJWA_001821"
     CDS             4016..4261
                     /gene="nrdH"
                     /locus_tag="ACHJWA_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFO4308437.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            4258..4668
                     /gene="nrdI"
                     /locus_tag="ACHJWA_001822"
     CDS             4258..4668
                     /gene="nrdI"
                     /locus_tag="ACHJWA_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFO4308438.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            4641..6785
                     /gene="nrdE"
                     /locus_tag="ACHJWA_001823"
     CDS             4641..6785
                     /gene="nrdE"
                     /locus_tag="ACHJWA_001823"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO4308439.1"
                     /translation="MATTTAECLTQKTMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADVAISQHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTGKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            6795..7754
                     /gene="nrdF"
                     /locus_tag="ACHJWA_001824"
     CDS             6795..7754
                     /gene="nrdF"
                     /locus_tag="ACHJWA_001824"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO4308440.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEYEDWNF"
     gene            8110..9312
                     /gene="proV"
                     /locus_tag="ACHJWA_001825"
     CDS             8110..9312
                     /gene="proV"
                     /locus_tag="ACHJWA_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFO4308441.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPNELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEIINNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            9305..10369
                     /gene="proW"
                     /locus_tag="ACHJWA_001826"
     CDS             9305..10369
                     /gene="proW"
                     /locus_tag="ACHJWA_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFO4308442.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            10426..11418
                     /gene="proX"
                     /locus_tag="ACHJWA_001827"
     CDS             10426..11418
                     /gene="proX"
                     /locus_tag="ACHJWA_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFO4308443.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            11610..12794
                     /locus_tag="ACHJWA_001828"
     CDS             11610..12794
                     /locus_tag="ACHJWA_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4308444.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISNKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            12918..13655
                     /gene="ygaZ"
                     /locus_tag="ACHJWA_001829"
     CDS             12918..13655
                     /gene="ygaZ"
                     /locus_tag="ACHJWA_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFO4308445.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            13645..13980
                     /gene="ygaH"
                     /locus_tag="ACHJWA_001830"
     CDS             13645..13980
                     /gene="ygaH"
                     /locus_tag="ACHJWA_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFO4308446.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            14071..14601
                     /gene="emrR"
                     /locus_tag="ACHJWA_001831"
     CDS             14071..14601
                     /gene="emrR"
                     /locus_tag="ACHJWA_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFO4308447.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            14728..15900
                     /gene="emrA"
                     /locus_tag="ACHJWA_001832"
     CDS             14728..15900
                     /gene="emrA"
                     /locus_tag="ACHJWA_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFO4308448.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQQMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            15917..17455
                     /gene="emrB"
                     /locus_tag="ACHJWA_001833"
     CDS             15917..17455
                     /gene="emrB"
                     /locus_tag="ACHJWA_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFO4308449.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(17519..18034)
                     /gene="luxS"
                     /locus_tag="ACHJWA_001834"
     CDS             complement(17519..18034)
                     /gene="luxS"
                     /locus_tag="ACHJWA_001834"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFO4308450.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     VWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(18184..19740)
                     /gene="gshA"
                     /locus_tag="ACHJWA_001835"
     CDS             complement(18184..19740)
                     /gene="gshA"
                     /locus_tag="ACHJWA_001835"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFO4308451.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIDKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            complement(19813..20241)
                     /gene="yqaA"
                     /locus_tag="ACHJWA_001836"
     CDS             complement(19813..20241)
                     /gene="yqaA"
                     /locus_tag="ACHJWA_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFO4308452.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFCPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(20238..20804)
                     /gene="yqaB"
                     /locus_tag="ACHJWA_001837"
     CDS             complement(20238..20804)
                     /gene="yqaB"
                     /locus_tag="ACHJWA_001837"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFO4308453.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(21085..21161)
                     /locus_tag="ACHJWA_001838"
     tRNA            complement(21085..21161)
                     /locus_tag="ACHJWA_001838"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21125..21127),aa:Arg,seq:acg)
     gene            complement(21264..21340)
                     /locus_tag="ACHJWA_001839"
     tRNA            complement(21264..21340)
                     /locus_tag="ACHJWA_001839"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21304..21306),aa:Arg,seq:acg)
     gene            complement(21405..21481)
                     /locus_tag="ACHJWA_001840"
     tRNA            complement(21405..21481)
                     /locus_tag="ACHJWA_001840"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21445..21447),aa:Arg,seq:acg)
     gene            complement(21485..21577)
                     /locus_tag="ACHJWA_001841"
     tRNA            complement(21485..21577)
                     /locus_tag="ACHJWA_001841"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21541..21543),aa:Ser,seq:gct)
     gene            complement(21893..22078)
                     /gene="csrA"
                     /locus_tag="ACHJWA_001842"
     CDS             complement(21893..22078)
                     /gene="csrA"
                     /locus_tag="ACHJWA_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFO4308454.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(22313..24943)
                     /gene="alaS"
                     /locus_tag="ACHJWA_001843"
     CDS             complement(22313..24943)
                     /gene="alaS"
                     /locus_tag="ACHJWA_001843"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFO4308455.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFVVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(25071..25571)
                     /gene="recX"
                     /locus_tag="ACHJWA_001844"
     CDS             complement(25071..25571)
                     /gene="recX"
                     /locus_tag="ACHJWA_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFO4308456.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(25639..26700)
                     /gene="recA"
                     /locus_tag="ACHJWA_001845"
     CDS             complement(25639..26700)
                     /gene="recA"
                     /locus_tag="ACHJWA_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFO4308457.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(26780..27277)
                     /gene="pncC"
                     /locus_tag="ACHJWA_001846"
     CDS             complement(26780..27277)
                     /gene="pncC"
                     /locus_tag="ACHJWA_001846"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFO4308458.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITLRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(27422..28507)
                     /gene="mltB"
                     /locus_tag="ACHJWA_001847"
     CDS             complement(27422..28507)
                     /gene="mltB"
                     /locus_tag="ACHJWA_001847"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFO4308459.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDRMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            28762..29324
                     /gene="srlA"
                     /locus_tag="ACHJWA_001848"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             28762..29324
                     /gene="srlA"
                     /locus_tag="ACHJWA_001848"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: protein motif:HMM:NF015565.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            29321..30282
                     /gene="srlE"
                     /locus_tag="ACHJWA_001849"
                     /gene_synonym="gutE"
                     /pseudo
     CDS             29321..30282
                     /gene="srlE"
                     /locus_tag="ACHJWA_001849"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
     gene            30293..30664
                     /gene="srlB"
                     /locus_tag="ACHJWA_001850"
                     /gene_synonym="gutB"
     CDS             30293..30664
                     /gene="srlB"
                     /locus_tag="ACHJWA_001850"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFO4308460.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            30668..31447
                     /gene="srlD"
                     /locus_tag="ACHJWA_001851"
     CDS             30668..31447
                     /gene="srlD"
                     /locus_tag="ACHJWA_001851"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFO4308461.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGTAYGFGADATSEQSVLALSRGVDEIFVRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            31553..31912
                     /gene="gutM"
                     /locus_tag="ACHJWA_001852"
     CDS             31553..31912
                     /gene="gutM"
                     /locus_tag="ACHJWA_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFO4308462.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SRGRFKPRVVVAIALDDQQRIADTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            31979..32752
                     /gene="srlR"
                     /locus_tag="ACHJWA_001853"
                     /gene_synonym="gutR"
     CDS             31979..32752
                     /gene="srlR"
                     /locus_tag="ACHJWA_001853"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFO4308463.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVGFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDADIDPAFRQALEEKGIDVIITGESNE"
     gene            32745..33710
                     /gene="gutQ"
                     /locus_tag="ACHJWA_001854"
     CDS             32745..33710
                     /gene="gutQ"
                     /locus_tag="ACHJWA_001854"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFO4308464.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTVGGTTL
                     QSQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(33707..35221)
                     /gene="norR"
                     /locus_tag="ACHJWA_001855"
     CDS             complement(33707..35221)
                     /gene="norR"
                     /locus_tag="ACHJWA_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFO4308465.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRSGDEVILEAQHFAFPEVTLPPPEVAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            35408..36847
                     /gene="norV"
                     /locus_tag="ACHJWA_001856"
     CDS             35408..36847
                     /gene="norV"
                     /locus_tag="ACHJWA_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFO4308466.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            36844..37977
                     /gene="norW"
                     /locus_tag="ACHJWA_001857"
     CDS             36844..37977
                     /gene="norW"
                     /locus_tag="ACHJWA_001857"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFO4308467.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLLLFPQTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKMVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPV"
     gene            complement(38105..40357)
                     /gene="hypF"
                     /locus_tag="ACHJWA_001858"
     CDS             complement(38105..40357)
                     /gene="hypF"
                     /locus_tag="ACHJWA_001858"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFO4308468.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPYLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALEDLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQVVLSQHLGDLSDDGIQTQWREALRLMQNIYNFTPQYVV
                     HDAHPGYVSCQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(40510..41037)
                     /gene="hydN"
                     /locus_tag="ACHJWA_001859"
     CDS             complement(40510..41037)
                     /gene="hydN"
                     /locus_tag="ACHJWA_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFO4308469.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(41186..42196)
                     /gene="ascG"
                     /locus_tag="ACHJWA_001860"
     CDS             complement(41186..42196)
                     /gene="ascG"
                     /locus_tag="ACHJWA_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFO4308470.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSVERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHKRGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            42456..>42614
                     /locus_tag="ACHJWA_001861"
     CDS             42456..>42614
                     /locus_tag="ACHJWA_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
                     /protein_id="HFO4308471.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDALIDS
                     PTLKTIPGV"
BASE COUNT        10140 a        11452 c        10759 g        10263 t
ORIGIN      
        1 tgagtaattt agattattgg tgaacagaac ggcgctctct gctcgctaat tccgcaagcc
       61 tgacaaaacc aaagtgaata aaaatgcaat ataacagaat aaaataaaac acccctcact
      121 ggtgctttct gctgttaatg tacattttct ggcataaaaa atgctgtaaa tgacccggtc
      181 acgacataca ccgaattctc tacgcttata gcgttacctc acccccaaac tttcattagt
      241 gaaattaaag gaaaggtcat atccaattga tttaattgaa aataaatcat atcggcatgt
      301 aaaaatccac aatgcacaaa aaacgagccg ttacggaata ttttatctac aaaaactgac
      361 taaataaaaa tttttcacta attgattagt catagccagc gatatacgct atgcgaaaat
      421 gcagatggca atgagatcca ctgctttcat ctccattaac atcccattac gcttttatta
      481 aggagcatta gcatgttctc accgcagtca cgcttgcgtc atgcagttgc agatacgttc
      541 gcgatggttg tttactgttc tgtcgtgaac atgtgtattg aagttttcct ctccggaatg
      601 agcttcgaac agtcttttta ttccagattg gtagcgattc cggtgaacat cttaattgca
      661 tggccatacg gtatgtaccg cgatctgttt atgcgcgcgg cacgcaaagt tagcccgtcg
      721 ggctggataa aaaatctggc ggatatcctg gcttatgtga cgttccagtc accggtgtat
      781 gtggcgatct tgttagtggt gggcgcagac tggcatcaga ttatggcggc ggtcagttca
      841 aacatcgttg tttcgatgtt gatgggggcg gtttatggct acttcctcga ttattgccgc
      901 cgactgttta aagtcagccg ttaccagcag gtaaaagcct gactcttctt ttcgcgactg
      961 gcatcgccag tcgcgtcttt aactggcttc gccaaataaa ccattcaaat aacgttcaag
     1021 cgcaacacgc gaactaaagc catgcgggat accgtaatgc tcgtgtcgct cgcctgctaa
     1081 atagagcgga aattgctggt aatgatcgca agttttaata tcggaagcgg gttcagccag
     1141 tgtcgaactg cgttctttta atgttggatg aattaccaga gcggtacgtc ccattcgcgc
     1201 ttcgcgatta acatagacat aattttcccc acggcggtaa ccatacgctt tctgggttac
     1261 cacatcgaca gtaaaaccga ctttttcaag tacgcgcgcc acctcgtctg gtcgtaaata
     1321 catatttgat cctcgttatt atcaaccgcg ggcctacctt acctgattgc gcattcacaa
     1381 cgctttcaga aaagtccata aagccgcgat gaacctcagt taagaaaatt atggtctaca
     1441 ctgaaaatta catcgaattc taatggggga tcatatgttt aaccgaccga accgcaacga
     1501 cgttgatgat ggcgtgcagg atatccagaa tgatgtcaat caattagctg acagcctcga
     1561 atctgtattg aagtcctggg gcagcgacgc caaaggggaa gctgaagccg cacgcagtaa
     1621 agctcaggca ttgctgaaag aaaccagggc gcgaatgcat ggtcgtactc gcgtccagca
     1681 agccgcgcgc gatgccgtag gctgcgcgga ttcttttgtt cgtgaaagac cctggtgtag
     1741 tgtgggaact gctgctgcgg taggtatttt tatcggtgcg ttgttgagca tgcgtaaatc
     1801 gtagtgagac gatgacaagc agtagtctga cttctgaaaa gtactcagtg cgtcaacgcc
     1861 ttgtccgcct acaggcctga aaaacatagc gcatcagggg attttgccct ggtcaccagt
     1921 cttataggac cccgaagcct ggcttcgggg ttattaattt aaagcactgc gcaattgctg
     1981 tgctacctgt cgcgccatac tttcgctaac gatattcgta aacgagagca ataatcctcc
     2041 gtcctcgctt gagcggatac gccagtcgct aagtgcctgt acggcaagac cagcagcatt
     2101 ggctttatgc acgaatgtca cgtcattacc tgctagtttg atcaccatct ggataccgcc
     2161 tttttgcggc acaacctgaa atccctgctg cgtaagtgcc tgctcaatcc actgccgtcg
     2221 ctgggcataa tgctggcgca ttttcttcag atgccgccag aaatgcccct cacggatgaa
     2281 atctgccagt gtgttctgcc acagtacggg tacagcacat ggttccagtg acgcctgctg
     2341 ccggaattgc gaaatctgct ccaccggcac caccagccac gcacagcgca atgcaggaaa
     2401 tagcgctttg ctgaatgttc cggcataaat cacccgctgc ggcgcgtcga gactttttag
     2461 tgccggtaac ggcttaccgt gatagcgaaa ctcactgtcg taatcatctt caataatcca
     2521 tgcctgacta cgctctgccc attccagtat ctgataccta cgcgctaaag agagtgccac
     2581 acccagcggg ctttggtgtg ccggagtaat cagggcaaaa cgtgcatcag gataattttg
     2641 tattccgctt gtgacatcca gtccgttgtc atcaaccggc acaggtaaaa tttccacacc
     2701 gtggcgagtg acaatcgggc gaatcagcgg aaagccggga tcttccatcc acatcccgtc
     2761 tcccggttgc gccagagcgt gcagaattaa agccattgag gccgcataac cgtgagtgat
     2821 aaagacctgc tctggctgac aatcaatccc acgtgaaacg cgtaaataat caacaatagc
     2881 ctcgcgcaag gccgcctcgc cgcagacatc gcctaatgcc aaatcaaagc gcgtctgggt
     2941 acgcagacgg cggcccatca cccgcgccca caattcacgc ggaaaaagat caagtgcagg
     3001 tagccccatc tgaaaaggca gcggctgctg actttcacca gcaaaagcca cgacatccac
     3061 agcagactgt tgcggatgta tctgctcact gacaaaggta cccgcctgtc cccgcctgac
     3121 cagccatccc tgcgccacca gctcagcata agcattttcg acagtggatc gagagacacc
     3181 cagctcctgc gaccaggtac ggctcgaagg tagccttgcg ccaggtttca gttttccttg
     3241 ctcgataacc gtttttaact gacgggcgat atcctgataa cgtggcatat ggtctgcttt
     3301 tttaggtaat ttatggctct ctttagcagt ccattatagt gctacattgc gggcgttaat
     3361 aagaggagaa actgtatgac tacattacgt cagccttact acgaacttag cccggcagtg
     3421 tataacgcac tggtgcaggc caaaacggca ctggaaaata gcacgctgga taccacgttg
     3481 atggagttaa tttatttgcg cgtctcgcaa atcaacggct gcgcattttg tctggagatg
     3541 cacagcaaag cattgcgcaa atccggtgtg ccacaacaca aactggacgc cctggcaggc
     3601 tggcgcgtaa gccatcattt tgatgaacgc gagcgagcgg cgctggcatg ggcggaatct
     3661 gtgaccgata ttgccagaac ccatgcggaa gacgaggttt atcagccttt gcttgagcat
     3721 ttcagcgcag cagaaatcag cgacttaacg tttgccatcg ggctgatgaa ttgttttaac
     3781 cgtctggccg tttccatgcg gatgtaattt gctgttgccg ggtatgcgcc cggcaaaaaa
     3841 acacaaaatc ccctaccccg ttgagccatt caccggtggc gtaatcaatc taaaattacc
     3901 atatcttgtg tggttgaatc ttttttcaac tacatctagt atctctgtat caacagagag
     3961 acaacccgac gcgtatcatc gcgccgtatc ttcattttaa acggaaatac gaatcatgcg
     4021 cattactatt tacactcgta acgattgcgt tcagtgccac gccaccaaac gggcgatgga
     4081 aaaccggggc tttgattttg aaatgattaa tgtcgatcgc gttcctgaag cggcagaagc
     4141 gttgcgtgct cagggctttc gtcagttgcc ggtagtgatt gctggcgatc ttagctggtc
     4201 tggtttccgt ccggacatga ttaaccgtct gcatccagcg ccacacgcgg ccagtgcatg
     4261 agccagctcg tctacttctc cagcagctcc gaaaacacgc agcgttttat cgaacgttta
     4321 ggtctgcccg cggtgcgcat cccgctcaat gagcgggaac ggattcaggt agacgagcct
     4381 tacatcctga tcgtgccctc ttacggcggc ggcggtacgg ctggcgcggt gccacgacag
     4441 gtaattcgct ttttaaacga cgagcacaac cgggcgttgc ttcgcggcgt tattgcttcg
     4501 ggtaatcgca actttggtga ggcgtatggc cgcgccggag atgtgattgc ccggaaatgc
     4561 ggcgtgccgt ggctgtaccg ttttgaactc atgggtacgc aaagcgatat cgaaaacgtt
     4621 cgtaaaggag taaccgaatt ttggcaacga caaccgcaga atgcctgacg cagaaaacga
     4681 tggattacca cgcgctgaat gcgatgctta acctctacga tagcgcaggt cgcattcagt
     4741 tcgataaaga ccgccaggcc gttgacgcct ttatggcgac gcatgtgcgt ccgaacagtg
     4801 tgaccttcag tagccagcag cagcgcctga actggctggt caacgaaggt tactatgatg
     4861 aaagcgttct taatcgctac tctcgcgact ttgtcattac gctgtttgcc cacgcacaca
     4921 ccagcggttt tcgtttccag acattcctcg gggcatggaa gttttacacc agctatacgt
     4981 tgaagacatt cgacggtaaa cgttatctgg aagattttgc cgatcgagtc acgatggtgg
     5041 cgctgacgct ggcacaaggc gatgagacgc tggcgttgca actgacagat gaaatgctgt
     5101 caggacgctt tcagccagcc acgccaacat tcctcaactg cggtaagcag cagcgcggcg
     5161 aactggtttc ctgttttttg ctgcgtattg aagacaatat ggagtcgatt ggtcgggcgg
     5221 taaattccgc actgcaactg tcgaaacgcg gcggcggcgt agcatttttg ctgtcgaatc
     5281 tgcgggaagc gggcgcgcca attaaacgta ttgaaaatca atcttctggc gtaattccgg
     5341 tgatgaaaat gctggaagac gcattttcct atgccaacca actcggcgct cgtcaggggg
     5401 ctggtgcagt ctatttacat gctcatcatc ccgatattct gcgttttctc gacacgaaac
     5461 gggaaaatgc cgacgaaaaa atccgcatta aaacactgtc gcttggcgtg gtgatcccgg
     5521 atatcacttt ccatctggca aaagagaatg cgcagatggc gctgttttcg ccttatgacg
     5581 tagagcgagt ttatggcaag ccgtttgccg atgtggccat cagccaacac tatgacgaac
     5641 tggttgccga tgaacgcatt cgcaaaaaat acctcaacgc ccgtgatttc ttccagcgac
     5701 tggcagaaat ccagtttgag tccggctatc cctacatcat gtatgaagac acggtaaacc
     5761 gtgctaaccc tgtcgccggg cgcataaata tgagtaatct ctgctcagaa attttgcagg
     5821 ttaacagcgc ctcagagtat gacgagaatc tcgactatgc ccgcacaggc catgatatct
     5881 cctgcaattt aggttcgttg aatattgcgc acaccatgga ttcccccgat tttgcccgca
     5941 cggtagagac tgccgtgcgc ggtttaacgg cagtatcaga tatgagtcat atccgcagcg
     6001 tgccgtccat cgaagccgga aatgccgcct cgcacgccat cggactgggg cagatgaatt
     6061 tacacggcta tctggcgcga gaaggcatcg cttatggttc gccggaagca ctggatttca
     6121 ccaatctcta tttctatgcc atcacctggc acgcactgcg tacctcgatg ttgctggcac
     6181 gcgaacgcgg tgaaaccttc gccgggttca aacagtcacg ctatgccagt ggtgaatatt
     6241 ttagccaata tctgcaaggg aactggcagc cgaaaacggg gaaagttggc gaactgtttg
     6301 cccgtagcgg tattacgtta cctacccgtg agatgtgggc gcaactgcgc gacgacgtga
     6361 tgcgctacgg catttacaac cagaatcttc aggcggtgcc gccaacaggt tctatctctt
     6421 atatcaacca tgctacgtcg agtattcatc cgattgtggc gaaagtagag atacgcaaag
     6481 agggtaaaac aggacgcgtt tactaccctg ccccgtttat gactaacgag aatctggcgc
     6541 tgtatcagga cgcttacgaa attggcgcag aaaagatcat cgacacctac gcggaagcga
     6601 ctcgccatgt cgatcagggg ctgtcgctga cgctgttttt ccccgatacc gccaccactc
     6661 gcgatatcaa caaagcgcag atttacgcct ggcgcaaagg tatcaaaacg ctctattaca
     6721 tccggctgcg tcagatggcg ctggaaggca ctgaaattga aggctgcgtc tcctgtgcac
     6781 tttaaggaat atctatgaaa ctctcacgta tcagcgccat caactggaac aagatatctg
     6841 acgataaaga tctggaggtg tggaatcgcc tgaccagcaa tttctggcta ccagaaaagg
     6901 tgccgctgtc gaacgatatt cctgcctggc agacattaac tgtcgtagaa caacaactga
     6961 cgatgcgcgt ttttactggc ctgacgctgc tcgacacgct gcaaaatgtt atcggcgcgc
     7021 cttcgctgat gcccgatgca ctcacgcctc atgaagaagc ggtattatcg aatatcagct
     7081 ttatggaagc ggttcatgcc cgctcttaca gttcgatttt ctcgacgctg tgccagacca
     7141 aagatgtcga tgccgcctac gcctggagtg aagaaaacgc gccgttgcag cgaaaagcac
     7201 agattattca gcaacattac cgcggtgatg atccgctgaa aaagaaaatc gccagtgtgt
     7261 ttcttgaatc ttttttgttc tattccggtt tctggctgcc gatgtatttt tccagccgcg
     7321 gaaagctgac caataccgcg gacctgatcc gtctgattat ccgcgatgaa gcagtccacg
     7381 gttactacat aggctataaa tatcagaaaa acatggaaaa gatatctctg ggacaacgtg
     7441 aagagttgaa gagtttcgcc ttcgatttgt tgctggaact ctacgacaac gagttgcaat
     7501 acaccgatga gctgtacgcc gaaaccccgt gggctgacga tgtgaaagcg tttctctgtt
     7561 acaacgccaa taaggctttg atgaatctgg gctacgaacc gttatttccc gcagaaatgg
     7621 cggaagtgaa tccggcaatc ctcgccgcgc tttcgccgaa tgccgatgaa aatcacgatt
     7681 tcttttccgg ttcaggctcc tcttatgtga tggggaaagc ggttgaaaca gaatatgaag
     7741 actggaattt ctgagggtgt tattttcaaa aatatcacta cccgcgacag ggaaataatt
     7801 cccgccaaat agctttttat cacgcaaata ttttgtggtg atctacactg atactctgtt
     7861 gcattattcg cctgaaacca caatattcag gcgttttttc gctatctttg acaaaaaata
     7921 tcaacttttc tcgatttgct ctcagccctt atatcacggg aaattccggc gatttgctcg
     7981 catcaatatt catgccacat ttgccatcag gggttgcctc agattctcag tatgttaggg
     8041 tagaaaaaag tgactatttc cattgggtaa tatatcgaca taaacaaata acaggaatct
     8101 ttctattgca tggcaattaa attagaaatt aaaaatcttt ataaaatatt tggtgagcat
     8161 ccacagcgag cgttcaaata tatcgaacaa ggactttcaa aagaacaaat tctggaaaaa
     8221 actgggctat cgcttggcgt aaaagacgcc agtctggcca ttgaagaagg cgagatattt
     8281 gtcatcatgg gattatccgg ctcgggtaaa tccacaatgg tacgccttct caatcgcctg
     8341 attgaaccca cccgcgggca agtactgatt gatggtgtgg atattgccaa aatatccgac
     8401 gccgaactcc gtgaggtgcg cagaaaaaag attgcgatgg tcttccagtc ctttgcctta
     8461 atgccgcata tgaccgtgct ggacaatact gcgtttggta tggaattggc cggaattaat
     8521 gccgaagaac gccgggaaaa agcccttgat gcactgcgtc aggtcgggct ggaaaattat
     8581 gcccacagct acccgaatga actctctggc gggatgcgtc aacgtgtggg attagcccgc
     8641 gcgttagcga ttaatccgga tattttatta atggatgaag ccttctcggc gctcgatcca
     8701 ttaattcgca ccgagatgca ggatgagctg gtaaaattac aggcgaaaca tcagcgcacc
     8761 attgtcttta tttcccacga tctcgatgaa gccatgcgta ttggcgaccg aattgccatt
     8821 atgcaaaatg gtgaagtggt acaggtcggc acaccggatg aaattatcaa taatccggcg
     8881 aatgattatg tccgtacctt cttccgtggc gttgatatta gtcaggtatt cagtgcgaaa
     8941 gatattgccc gccggacacc gaatggctta attcgtaaaa cccctggctt cggcccacgt
     9001 tcggcactga aattattgca ggatgaagat cgtgaatatg gctacgttat cgaacgcggt
     9061 aataagtttg tcggcgcagt ctccatcgat tcgcttaaaa ccgcgttaac gcagcagcaa
     9121 ggtcttgatg cggcgctgat tgatgcgccg ttagcagtcg atgcacaaac gcctcttagc
     9181 gagttgctct ctcatgtcgg acaggctccc tgtgcggtgc ccgtggtcga cgaggaccaa
     9241 cagtatgtcg gcatcatttc gaaaggaatg ctgctgcgcg ctttagatcg tgagggggta
     9301 aataatggct gatcaaaata atccgtggga taccacgcca gcggcggaca gtgccgcgca
     9361 atccgcagac gcctggggta caccggcgac tgcaccgact gacggcggtg gtgctgactg
     9421 gctgaccagt acgcctgcgc caaacgtcga gcattttaat attctcgatc cgttccataa
     9481 aacgctgatc ccgctcgaca gttgggtcac tgaagggatc gactgggtcg ttacccattt
     9541 ccgtcccgtc ttccagggcg tgcgcgttcc ggttgattat atcctcaacg gtttccagca
     9601 attgctgctg ggtatgcccg caccggtggc gattatcgtt ttcgctctca tcgcctggca
     9661 gatttccggg gtcggaatgg gcgtggcgac gctggtttcg ctgattgcca tcggcgcaat
     9721 cggtgcctgg tcgcaggcaa tggttactct ggcgctggtg ttaaccgccc tgctgttctg
     9781 tatcgtcatc ggtttgccgt tggggatctg gctggcgaga agtccgcgag cggcgaaaat
     9841 tattcgtcca ctgcttgatg ccatgcagac cacgccagcg tttgtttatc tggtgccaat
     9901 cgtcatgcta tttggtatcg gtaacgtgcc gggcgtggtg gtgacgatca tctttgctct
     9961 gccgccgatt atccgtctga ccattctggg gattaaccag gttccggcgg atctgattga
    10021 agcctcgcgc tcattcggtg ccagcccgcg ccagatgctg ttcaaagttc agttaccgct
    10081 ggcgatgccg accattatgg cgggcgttaa ccagacgctg atgctggccc tttctatggt
    10141 ggtcatcgcc tcgatgattg ccgtcggcgg gttgggtcag atggtacttc gtggtatcgg
    10201 tcgtctggat atggggcttg ccaccgttgg cggcgtcggg attgtgatcc tcgccattat
    10261 cctcgatcgt ctgacgcagg ccgttgggcg cgactcacgc agtcgcggca accgtcgctg
    10321 gtacaccact ggccctgtcg gtctgctgac ccgcccattc attaagtaac tctgcacttg
    10381 cccggtgacg ccgggcatat caccctgcca aaaaaaggaa taacaatgcg acatagcgta
    10441 ctttttgcga cagcgtttgc cacgcttatc tctacacaaa cttttgctgc cgacctgccg
    10501 ggcaaaggca ttactgttaa tccggttcag agcaccatca ccgaagaaac tttccagacg
    10561 ctgctggtca gccgcgcgct ggagaaatta ggttataccg tcaataaacc cagcgaagtg
    10621 gattacaacg ttggctacac ctcgcttgct tccggcgatg caaccttcac cgccgtgaac
    10681 tggacgccac tgcatgacaa catgtacgaa gctgccggtg gcgataagaa attttatcgt
    10741 gaaggggtat ttgttaacgg cgcggcacag ggttacctga tcgataagaa aaccgccgac
    10801 cagtacaaaa tcaccaacat cgcacaactg aaagatccga agatcgccaa actgttcgat
    10861 accaacggcg acggaaaagc ggatttaacc ggttgtaacc ctggctgggg ctgcgaaggt
    10921 gcgatcaacc accagcttgc cgcgtatgaa ctgaccaata ccgtgacgca taatcagggg
    10981 aactacgcgg cgatgatggc cgacaccatc agtcgttaca aagagggcaa accggtgttt
    11041 tactacacct ggacgccgta ctgggtgagt aatgagctga agccaggcaa agatgtggtc
    11101 tggttgcagg tgccgttctc tgcactgccg ggcgataaaa atgccgatac caaactgccg
    11161 aatggcgcga attacggctt cccggtcagc accatgcata tcgttgccaa taaagcctgg
    11221 gccgagaaaa acccggcagc agcgaaactg tttgccatta tgcagttgcc agtggcagat
    11281 attaacgccc agaacgccat tatgcatgac ggcaaagcct cagaaggcga tattcagggc
    11341 cacgttgatg gctggatcaa agcccaccag caacagttcg atggctgggt gaatgaggcg
    11401 ttggcagcgc agaagtaatt tttattcggg cggataaggc gtttacgccg catccgccgt
    11461 tctgtacaca atgcctgatg cgacgctggc gcgtcttatc atgcctacaa accacaccgc
    11521 acatttcaac aatctatctt tcatcccata ttcatcaaca tccgctatta ttgatttcca
    11581 gcttaatcat cacctgatga acaaaaataa tgaccaaacc taatcatgag cttagcccgg
    11641 cgctaatcgt gctgatgtct atcgccaccg gtctggcggt cgccagcaac tattacgccc
    11701 agccattgct cgacaccatc gcgcgtaact tttccctttc cgccagttcg gcaggcttta
    11761 ttgttaccgc cgcgcagttg ggctatgccg ccggtctgct gtttcttgtt cccctcggtg
    11821 atatgtttga acgccgccgc ctgattgtct cgatgacctt actggcagcg ggcggtatgt
    11881 taatcactgc cagcagtcag tcactggcga tgatgatcct cggtacggca ttaaccggtt
    11941 tattctcagt cgtggcacaa attctggttc cgctggcagc gacgctggct tcaccggata
    12001 aacgcggcaa agtggttggc accattatga gcggtctgct gttggggata ttgctggcac
    12061 gaaccgttgc cgggttactg gcaaacctcg gcggctggcg caccgtcttt tgggttgcct
    12121 cggtgttaat ggctctgatg gcgctggcat tatggcgtgg tctgccacaa atgaaatcag
    12181 aaacccacct caactaccca cagttgttgg gttccgtttt cagcatgttt atcagcaata
    12241 agatcctgcg cacccgcgcg ttgctgggct gcctgacctt tgccaacttc agcattctct
    12301 ggacctcaat ggcctttttg cttgccgctc caccttttaa ctacagcgat ggtgtaattg
    12361 gtctgtttgg gcttgcggga gctgccggag cgttgggcgc tcgtccggcg ggcggttttg
    12421 ccgacaaggg caaatcgcac cacaccacaa ctttcggtct gcttctgctg ttactttcat
    12481 ggctggcaat ctggtttggt cacacttccg tactggcgtt gattatcggc attctggtgc
    12541 tggacctcac cgtacagggc gtgcatatca ctaaccagac ggtaatttat cgaatacatc
    12601 ctgatgcgcg taatcgcctg accgcaggtt acatgaccag ctactttatt ggcggtgccg
    12661 ccggttcgct aatttcagcc tcagcctggc aacatggcgg ttgggctggc gtttgtctgg
    12721 ctggcgcgac gattgccctg gttaacttac tggtctggtg gcgaggtttt catcgtcagg
    12781 aagccgcaaa ttaagcaatc agcaaccgtt ttcgtggctt tacacttata agggtgttaa
    12841 gaagcccatc agtctgataa ggttaagata ttcattcagt ctatttataa tattaacaat
    12901 cgttaagcgt acactctatg gaaagcccta ctccacagcc tgctcctggt tcggcgacct
    12961 tcatggaagg atgcaaagac agtttaccga ttgttattag ttatatcccg gtggcctttg
    13021 cgttcggtct gaatgcgacc cgtctgggat tctctcctct cgaaagcgtt tttttctcct
    13081 gcatcattta tgcaggcgcg agccagttcg tcattaccgc gatgctggca gcagggagta
    13141 gtttgtgggt tgctgcactg accgtcatgg caatggatgt tcgccatgtt ttgtatggcc
    13201 cgtcactgcg tagccgtatt attcagcgtc tgcaaaaatc gaaaaccgcc ctatgggcgt
    13261 ttggcctgac ggatgaggtt tttgccgccg ccaccgcaaa actggtacgc aataatcgcc
    13321 gctggagcga gaactggatg atcggcattg ccttcagttc atggtcatcg tgggtatttg
    13381 gtacggtaat aggggcattc tccggcagcg gcttgctgca aggttatccc gccgttgaag
    13441 ctgcattagg ttttatgctt ccggcactct ttatgagttt cctgctcgcc tcttttcagc
    13501 gcaaacaatc tctttgcgtt accgcagcgt tagttggtgc ccttgcaggc gtaacgctat
    13561 tttctattcc cgtcgccatt ctggcaggca ttgtctgtgg ctgcctcact gcgttaatcc
    13621 aggcattctg gcaaggagcg cccgatgagc tatgaggttc tgctgcttgg gttactggtt
    13681 ggcgcggcga attattgctt ccgctatttg ccgctgcgcc tgcgtgtggg taatgcccgc
    13741 ccaaccaaac gtggcgcggt aggtattttg ctcgacacca ttggcatcgc ctcgatatgc
    13801 gctctgctgg ttgtctctac cgcaccagaa gtgatgcacg atacacgccg tttcgtgccc
    13861 acgctggtcg gcttcgcggt actgggtgcc agtttctata aaacacgcag cattatcatc
    13921 ccaacactgc ttagtgcgct ggcctatggg ctcgcctgga aagtgatggc gattatataa
    13981 cccacaagaa tcatttttct aaaacaatac atttacttta tttgtcactg tcgttactat
    14041 atcggctgaa attaatgagg tcatacccaa atggatagtt cgtttacgcc cattgaacaa
    14101 atgctaaaat ttcgcgccag ccgccacgaa gattttcctt atcaggagat ccttctgact
    14161 cgtctttgca tgcatatgca aagcaagctg ctggagaacc gcaataaaat gctgaaggct
    14221 caggggatta acgagacgtt gtttatggcg ttgattacgc tggagtctca ggaaaaccac
    14281 agtattcagc cttctgaatt aagttgtgct cttggatcat cccgtaccaa cgcgacgcgt
    14341 attgccgatg aactggaaaa acgcggttgg atcgaacgtc gtgaaagcga taacgatcgc
    14401 cgctgcctgc atctgcaatt aacggaaaaa ggtcacgagt ttttgcgcga ggttttacca
    14461 ccgcagcata actgcctgca tcaactctgg tccgcgctca gcacaacaga aaaagaccag
    14521 ctcgagcaaa tcacccgcaa attgctctcc cgtctcgacc agatggaaca agacggtgtg
    14581 gttctcgaag cgatgagcta acgcgtcatc tcgctcaaaa atccagattt ataaaagaaa
    14641 aaatgaccgg ccagcatcgc aacatgctgg cctttttggc aagcaggtcg gctcagccga
    14701 tgagttaaga agatcgtgga gaacaatatg agcgcaaatg cggagactca aaccccgcag
    14761 caaccggtaa agaagagcgg caaacgtaag cgtctgctcc tccttctcac cttgctcttt
    14821 ataattattg ccgtagcgat agggatttat tggtttttgg tactgcgtca cttcgaagaa
    14881 accgatgacg catacgtggc agggaatcaa attcaaatta tgtctcaggt gtctggcagc
    14941 gtgacgaaag tctgggccga taacaccgat tttgtaaaag aaggcgacgt gctggtcact
    15001 ctcgacccga cagatgctcg ccaggcgttt gaaaaagcca aaactgcact ggcttccagc
    15061 gttcgccaaa cccaccagca gatgattaac agcaagcagt tgcaggcgaa tattgaggtg
    15121 caaaaaatcg ccctcgcgaa agcacaaagc gactacaacc gccgtgtgcc gctgggcaat
    15181 gccaacctga ttggtcgcga agagctgcaa cacgcccgcg acgccgttac cagtgcccag
    15241 gcgcaactgg acgtcgcgat tcaacaatac aatgccaatc aggcgatgat tctggggact
    15301 aaactggaag atcagcctgc cgtgcaacag gctgccaccg aagtacgtaa cgcctggctg
    15361 gcgctggagc gtactcgtat tgtcagtccg atgaccggtt atgtctcccg ccgcgcggta
    15421 cagcctgggg cgcaaattag cccaacgacg ccgctgatgg cggtcgttcc agccaccaat
    15481 atgtgggtgg atgccaactt taaagagacg cagattgcca atatgcgtat cggtcagccg
    15541 gtcactatca ccaccgatat ttacggcgat gatgtgaaat acaccggtaa agtggttggt
    15601 ctggatatgg gcacaggtag cgcgttctca ctgcttccgg cgcaaaatgc gaccggtaac
    15661 tggatcaaag tcgttcagcg tctgcctgtg cgtatcgaac tggaccagaa acagctggag
    15721 caatacccgc tgcgtatcgg tttatccacg ctggtgagcg tcaataccac taaccgtgac
    15781 ggtcaggtac tggcaaataa agtacgttcc actccggtag cggtaagcac cgcgcgtgaa
    15841 atcagcctgg cacctgtcaa taaactgatc gacgatatcg taaaagctaa cgctggctaa
    15901 tccagaggtg cgtgtgatgc aacagcaaaa accgctggaa ggcgcgcaac tggtcattat
    15961 gacgattgcg ctgtcactag cgacattcat gcaggtgctg gactccacca ttgctaacgt
    16021 ggcgatcccc actatcgccg ggaatcttgg ctcatcgctc agccagggaa cgtgggtaat
    16081 cacttctttc ggggtggcga atgccatctc gatcccgctt accggctggc tggcaaagcg
    16141 cgtcggggaa gtgaaactgt tcctctggtc aaccatcgcc tttgctattg cgtcgtgggc
    16201 gtgtggtgtc tccagcagcc tgaatatgct gatcttcttc cgcgtgattc aggggattgt
    16261 cgccgggccg ttgatcccgc tttcgcaaag tctattgctg aataattacc cgccagccaa
    16321 acgctcaatc gcgctggcgt tgtggtcgat gacggtgatt gtcgcgccaa tttgcggccc
    16381 aatcctcggc ggatatatca gcgataatta ccactggggc tggatattct tcatcaacgt
    16441 gccgattggc gtggcggtgg tgttgatgac actgcaaact ctgcgcggac gtgaaacccg
    16501 caccgaacgg cggcggattg atgccgtggg gctggcactg ctggttattg gtatcggcag
    16561 cctgcagatt atgctcgacc gcggtaaaga gctggactgg ttttcatcac aggaaattat
    16621 catccttacc gtggtggcgg tggtggctat ctgcttcctg atcgtctggg agctgaccga
    16681 cgataacccg atagtcgatc tgtcgttgtt taagtcgcgc aacttcacca tcggctgttt
    16741 gtgtatcagc ctcgcgtata tgctctactt cggcgcgatt gttctgctgc cgcagttgtt
    16801 gcaggaggtc tacggttaca cggcgacctg ggcaggtttg gcctctgcgc cggtagggat
    16861 tattccagtg atcctgtcgc cgattatcgg ccgcttcgcg cataaactgg atatgcgccg
    16921 actggtaacc ttcagcttta ttatgtatgc cgtctgcttc tactggcgtg cctatacctt
    16981 tgaaccaggt atggattttg gcgcgtcggc ctggccgcag tttatccagg ggtttgcggt
    17041 ggcctgcttc tttatgccgc tgaccaccat tacgctgtct ggtttgccac cggaacgact
    17101 ggcggcggca tcgagcctct ccaactttac gcgtacgctg gcggggtcta tcggcacgtc
    17161 gataaccaca accatgtgga ccaaccgcga gtcgatgcac catgcgcagt tgactgagtc
    17221 ggtaaacccg ttcaacccga atgcccaggc gatgtacagt caactggaag ggcttgggat
    17281 gacacaacag caggcatcag gctggattgc ccagcagatc accaatcagg ggctgattat
    17341 ttccgccaat gagatcttct ggatgtcggc cgggatattc ctcgtcttgc tggggctggt
    17401 gtggtttgct aaaccgccct ttggcgcagg tggcggcgga ggcggtgcgc actaagtaca
    17461 actaagccag ttcatttgaa ctggcttttt tcaattaatt gtgaagatag tttactgact
    17521 aaatgtgcag ttcctgcaac ttctctttcg gcagcgccag ttcttcgttg ctgttgatgc
    17581 gcacgtcacg ttccagaatg ctacgcgcaa tatcctgcgc ttcctgcaac gagtgcatct
    17641 ggtaagtgcc acactggtag acgttcagct ccggaatctg attctgatcc tgcactttca
    17701 gcacgtcttc cattgccgct ttccagacat cagcaacacg ctgctcatct ggcgtaccaa
    17761 tcagactcat ataaaaaccg gtgcggcagc ccattggcga gatatcgata atctctacac
    17821 cattaccgtt aagatggtta cgcataaaac cagcaaacag gtgctccagg gtatggatcc
    17881 ctctttctgg catcacttct ttgttcggca cgcagaagcg cagatcgaac acggtgattg
    17941 cgtcgccatg cggggtgttc attgttttcg ccacccgaac tgcaggcgct tccatccggg
    18001 tatgatcgac tgtgaagcta tctaacaacg gcatttagcc acctccggta atttttttaa
    18061 aaattttctg aactctttct tcccaggcga gtctgagtat atgaaagacg cgcatttgtt
    18121 atcatcatcc ctgaattcag agatgaaatt ttggccactc atgagtggcc tttttctttt
    18181 ctgtcaggcg tgtttttcca gccacaccgc aaacggttcg gtatctgcgg cttccatttc
    18241 ctgctgacgg cgttcagatg cctcgcgctc ggctacaaaa tcctcttcgc gcagaatttc
    18301 cagcggctct tcacgcagca gattacggta cgcttcagca aacgctttgc ctgttccgcc
    18361 aataccagta tcaatcatag accttaagat acgggcagag aaagtcagat cgggattatc
    18421 gaagcaggca accagttcat cacacacttt ctgatacgct tcgccgccgt taatgctatc
    18481 cagcgtttgc gcgacgcgtt tcagatcgcg gaacagatct ttacccacct gcggtaacgg
    18541 gaactgtgcg gtttcgcagc cgatacccag cgtcagaccc ggtttgcgac cttcgaggat
    18601 cacccggttc cagttaacgc gtgtacaggc aagttcgcta ctgctcattt ccggtgcatc
    18661 agccagcgca caccagacca taaacaggtc gaggaatcgc acctgctgtt catctacacc
    18721 aatcggcgag aacgggttga tgtccagcga acgcacttca atatattcaa tgccgccacg
    18781 taacagcgca tcagaaggcg actcgccgct gcgggtaacg cgttttggac gaatcggcgc
    18841 gtacagttcg ttttcaatct gcagcacgtt gctgttgatt tgcagcctct taccgtcttt
    18901 atcaatacca atcttcgcgt actcttccga tggcgttttg attgcctgtt tcaggcccgc
    18961 tacgtactcg taaagatcgt tgaaggtaat accaagattg ctttgcgatt tattggtata
    19021 gccgagatcg ctcaaacgaa gagaggtcgc atacggcagg taatacatac cgcactcggt
    19081 tttctcaaac ggcagcgacg ttggtttacc ttgcaggaat gaagagcata ttgccggaga
    19141 cgcgccaaac agataaggaa tgacccaacc gaagcgatag tagttgcgaa taacgcggaa
    19201 atagcccgca gagattttct ctttagcatc agcgcccgaa atatcgccac acttcgcttg
    19261 ccagaatgcc ataggcaaag agaagttgta atgcacgccg gagatggttt gcatcagcgc
    19321 gccgtagcga tttttcagcc cttcacggta cagcgtttta aagcgtccgg tgttagaagt
    19381 gccgtactgc gccagttcga tgtcctgacc ttctgcgatg tagcatggca tacttaacgg
    19441 ccacatccgc tcatcgccca tattgcgcgc cgtataacga tgcagatcgc gcataaaggt
    19501 cagcatatgt tcaatatcac catccactgg tgtaatgaat tccagcaatg cttccgcaaa
    19561 atcggtagta atccatttat gcgtcagtgc ggaacctaat gcttcaggat gacctgttgt
    19621 tgccagtgtg ccatcagcat taacacgcaa agtttcgcgc tccagcccac gctgtatccc
    19681 ctttaacgcc tgaggatgtt tttccagcca ggccagcgcc tgtgatacgt ccgggatcaa
    19741 attgacctcc cgcctgtcaa aatcgtttta attagcataa ctgtaatggt gaccatatgt
    19801 gcaggcctac aattagtgcc accacatcat gccctgaacg gtcgctgctg caactgcaac
    19861 atagcgtaac gctttaccaa ggcacaaaaa aaagattacc ggcccccagg agatgcgcat
    19921 ccatcccgct aacagacaca gtaaatcgcc aaccacgggc atccagctta ataatagcgt
    19981 gactgcacca tagcgtttca gccagccggt agctttctct tgccagcgcg atgttttacg
    20041 caatggacag aaacgcccaa ggataacgtt agttaaccct ccaaggctat tacccattgt
    20101 tgctgttaaa actaaaaccc agggatgact gatcccagaa agcaacattg ccaccagcac
    20161 gacttcggag ttgccgggta atagtgtagc gctgagaaaa ctactggcaa acaacgagaa
    20221 aagcgataac gcttcactca cagcaagcga acatccacag cgtccatgcc tgctgcacgg
    20281 gccgcctgaa taccgaaatc ggcatcttca aagaccacac actgcgtcgg ttgcacgccc
    20341 atacgctgcg cgcacaacaa aaatgtgtct ggcgcgggtt tatggtgttt gacgtgatcg
    20401 gcagcgacga cggcgtcaaa ataacggcgt aatcccaggt gagccagcaa tgcctcagcg
    20461 atggcgcttt cactccccgt tcctacagcc attgggcgac gaccatgcca acttttcacc
    20521 acttcaacaa gaggaagcgg ctcgacacta tccagcagca tacttcttac tgcttctgtt
    20581 ttttcacgcg ctaacgcatg cgggtcgaga tcggcctgat tcagctcaat aattgcctga
    20641 gcaatacgcc aggtgggcga tccattaagc gcaatcatcg cctgaacatc gtactgaaga
    20701 ccgtagtgcc ctaatacttc gcgccacgct ttacggtgcg taggctccgt atccaggatt
    20761 gtgccatcca tatcaaaaat taaacctgca taacgctcgt acatggtctt ctcgcaagtc
    20821 gaaaaatatg acgctacttt agcgtaattg cttgattttg tcgctgatgg cgaaggagaa
    20881 gaaaattcag gaaatggata aagtagttat ttagaaatga gatatttttg aaggaaattt
    20941 tttggagaag atggtgcatc cgggaggatg actcactggc gttcgccctt cgggccgttg
    21001 ctaaagcaac gttatccttc ctggtgcctg cggttaattc cgctacctct tcgatatctt
    21061 cattgctgaa gatttcggag aatatggtgc atccgggagg attcgaacct ccgaccgctc
    21121 ggttcgtagc cgagtactct atccagctga gctacggatg catcgggaaa atttatttta
    21181 ctgctgatat tgatatcgct actaaagcca tatcaaataa gaaaatccat cttaaccatc
    21241 atcactgatg attacggaga atatggtgca tccgggagga ttcgaacctc cgaccgctcg
    21301 gttcgtagcc gagtactcta tccagctgag ctacggatgc atcgggaaac ttactttact
    21361 gcagattttt tgataccgct actaaagccg tatcaagtaa gagatggtgc atccgggagg
    21421 attcgaacct ccgaccgctc ggttcgtagc cgagtactct atccagctga gctacggatg
    21481 caaatggcgg tgaggcgggg attcgaaccc cggatgcagc ttttgaccgc atactccctt
    21541 agcaggggag cgccttcagc ctctcggcca cctcaccaca cgcctcttac gagtgcttcg
    21601 aagaacttgt ttattgctca tcgtcgctgc gtggcgcaca tattactttc tgagacttat
    21661 aagtcaaaca atttttccca cacttttatc gtttgcacac ttcacgctca attagtctgt
    21721 aaaaacgtca aaaagagtgt tttatctaca gaaaaacgga ggtctggcag atagcagtaa
    21781 tgcaaaaaaa tggagactta agttgaatga acgggagtaa agcgaaaaga ctatagagtg
    21841 aaggagaaat tttgagggtg cgtctcaccg ataaagatga gacgcggaaa gattagtaac
    21901 tggactgctg ggatttttca gcctggatac gctggtagat ctcttcacgg tgaacagaaa
    21961 cttccttcgg ggcatttacg ccaatacgta cctggttgcc ctttacccct aaaactgtca
    22021 cggtgacctc atccccaatc atgagggtct caccaactcg acgagtcaga atcagcattc
    22081 tttgctcctt gaaagattaa aagagtcggg tctctctgta tcccggcatt atccatcata
    22141 taacgccaaa aagtaagcaa tgacaaacac attacatgta agcagtcatg gcattacatt
    22201 ctgttaaacc taagtttagc cgatatacac aacttcaacc tgactttatc gttgtcgata
    22261 gcgttaatgc gaatgccgtg aagcgagtcc acggcattgc ctgacgctta tattattgca
    22321 atttcgcgct gacccagcct ttcacactgg ctaacgctgc aggtaaggcc gcagcatccg
    22381 taccaccggc ttgcgccatg tcaggacgtc caccaccctt gccgcccacc tgctgagcga
    22441 ccataccaat cagttcccct gctttcacac gatctgtgac gtccttagat acgcctgcaa
    22501 tcagagaaac cttaccttcg gctaccgttg ccagcacgat aattgtcgac cccagctgat
    22561 tttttaaatc gtcaaccatg gtacgcaaca ttttcggctc aacaccgcta agctcgctaa
    22621 ccaacagctt aacaccatta acatcaattg ccttactgga aagatttgcg ctctcctgtg
    22681 cggcagcttg ttctttaagc tgttgtagtt ctttttctaa ctgacgcgta cgttccagta
    22741 ctgagcgcac tttatccgcc agattattgc tatcgccttt cagcagatgc gcgacttcgc
    22801 ttaagcgatc gctgtctgca tgaacggtgg tgatagcgcc ttctccggtt accgcttcga
    22861 tacgacgaac gcctgcagca gtacccgatt cagagatgat gcggaacaga ccaatatcac
    22921 cagtgcggct ggcgtgagta ccgccacaca gctcggtgga gaaatcgccc atgctcagca
    22981 cgcgtacacg ctcatcatac ttctcgccga acagcgccat cgcacctttc gctttcgccg
    23041 cttcgagatc catgatgttg gtttcgatcg gcaaattgcg acgaatctgt gcgttcacca
    23101 ggtcttcgac cgcacgaatc tcttccggtt tcatcgcttc gttgtgtgag aagtcgaagc
    23161 gcagcacctt gtcgttaacc agtgaacctt tctgcgatac atgagtcccc agaacctggc
    23221 gcagcgcagc gtgcatcagg tgcgttgcgg agtgattcaa acgaatacgg gcgcgacgag
    23281 cctcatcaac atcagcctgc acagcgtcgc ccactttcag agaacccgca gcaagtttac
    23341 cgatgtgacc aatcgcctgg ccgtatttct gcgtatcttc caccacgaag gagaagttag
    23401 cgcctttcag ttcgccttta tcaccaacct gaccgccgga ttccgcatag aatggcgttt
    23461 gatccagcac gaccacagct tcctggcctg cattgatggc atcaaccgct ttaccatcaa
    23521 caaacagcgc ggtcactttg ccgttcagtt ccagatggtc atagccttta aattcagatg
    23581 cactgtcaac acggatcatt gcgttgtaat cggcaccaaa gccgctggct tcgcgcgccc
    23641 gacgacgctg ctcttccatt gctgcttcaa aaccagcttc gtcaactttg atgttgcgct
    23701 cacgacaaac atcagccgtc aggtcaaccg ggaagccata ggtgtcgtac agacggaaag
    23761 cagtttcacc atccagcgta tcaccagaaa gttttgccag ctcttcatcc agcaacgcca
    23821 gaccgcgctc cagagtacga gcaaactgct cttcttcagt cttcagcacc tgctcaacct
    23881 gcgcctgctg gcgtttcagg tcttcacccg cagagcccat aacgtcgatc agcggaccaa
    23941 ccagtttgta gaagaaggtt tctttcgcgc cgagcatatt accgtgacgc actgcgcgac
    24001 gaatgatacg acgcagtaca taaccacggt tttcattgga cggcattacg ccatccgcga
    24061 tcaggaacgc acaagaacga atgtggtcag cgattacgcg cagcgattta ttgctcagat
    24121 cggttgcgcc agtgactttc gctaccgcct ggatcaacgt gcggaacagg tcgatgtcat
    24181 agttagagtt aacgtgttgc agcaccgcag caatacgctc cagacccata ccggtatcta
    24241 cagacggctt cggcagcggt tccatcgtgc catcggcctg gcggttgaac tgcatgaaga
    24301 cgatgttcca gatctcaatg tagcggtcgc cgtcttcttc cggacttccc ggagggccac
    24361 cccaaatgtg gtcgccgtga tcgtagaaga tttcggtgca cgggccgcac ggaccagtgt
    24421 cacccatctg ccagaagttg tcagatgcgt atggcgcacc tttgttatcg ccgatgcgaa
    24481 taatacgttc gcgcgggatc cctacttctt tttcccagat ttcgtaggct tcgtcgtcgc
    24541 tttcatagac ggtaacccac agacgctctt tcggcagggc aaaccatttt tcgctggtca
    24601 gcagttccca tgcaaactga atggcatcgt gtttgaaata gtcgccgaag ctgaagttgc
    24661 ccagcatttc gaagaaggta tggtgacgcg cggtgtaacc gacgttttcc aggtcgttgt
    24721 gtttaccacc cgcacgcacg cagcgttggg aagtggtagc gcgggaataa ttacgcttgt
    24781 cgagcccaag gaacacatcc ttgaactggt tcatcccggc gttggtaaac aacaaagttg
    24841 ggtcgttatg gggtaccagg gagctgctgg caactacctg atgtccctta ctatggaaaa
    24901 agtcgagaaa cgcctgacgg atctcagcgg tgctcttgct cataattatc ctgaaatcaa
    24961 gctaacgaaa tatcgccacc agctccagcg tgtcttaacc gccgggctgg taactgaaaa
    25021 gtgggaataa gataagtttt cttgactggg aagtaaaata ccgtatgcgt tcagtcggca
    25081 aaatttcgcc aaatatcctg gatatcttcc atcagatagc cacgatagag cagaaaacgc
    25141 tggatcttaa ctttttctga aaagacagtt ggcaaaggtt caccatattt tcgcgtcgcc
    25201 tgatcgcgcg ccagtgcgca ccagtcgatg tcacattcac gcatcgcttt ttctgtcgct
    25261 tcgcgggaaa tacctttctg attcagttcc tggcgaatac gcgcaggtcc ataacctttg
    25321 cggctacggc tggcgataaa gcgcgcaaca aatcggctgt catcgagata gccatgttca
    25381 tggcaccagg caataacgcg ctcgtaatct tctgccgtag catcaatctc ttctgggcca
    25441 tttttgccca taatcggtgc cgcgagttta cgtcgcagtt cttgctcact gtgatcgcgc
    25501 accgccagaa tgcgtaccgc acgatccaac aggcgagcat atgccgggcg acgggacgtt
    25561 gattctgtca tggcatatcc ttacaactta aaaagcaaaa gggccgcaga tgcgaccctt
    25621 gtgtatcaaa caagacgatt aaaaatcttc gttagtttct gctacgcctt cgctatcatc
    25681 tacagagaaa tccggcgttg agttcgggtt gctcagcagc aactcacgta ctttcttctc
    25741 gatctctttc gcggtttccg ggttatcttt cagccaggca gttgcattcg ctttaccctg
    25801 accgatcttc tcacctttgt agctgtacca cgcgcctgct ttctcgatca gcttctcttt
    25861 tacgcccagg tcaaccagtt cgccgtagaa gttgatacct tcgccgtaga ggatctggaa
    25921 ttcagcctgt ttaaacggcg cagcgatttt gttcttcacc actttcacgc gggtttcgct
    25981 acccaccacg ttttcgccct ctttcaccgc gccgatacga cggatgtcga gacgaacaga
    26041 ggcgtagaat ttcagcgcgt taccaccggt agtggtttcc gggttaccga acatcacacc
    26101 aattttcata cggatctggt tgatgaagat cagcagcgtg ttggactgct tcaggttacc
    26161 cgccagctta cgcatcgcct ggctcatcat acgtgccgca aggcccatgt gagagtcgcc
    26221 gatttcgcct tcgatttccg ctttcggcgt cagtgccgcc acggagtcaa cgacgataac
    26281 gtctactgcg ccagaacgcg ccagggcgtc acagatttcc agtgcctgct cgccggtgtc
    26341 cggctgggag cacagcaggt tgtcgatatc gacgcccagt ttacgtgcgt agattgggtc
    26401 cagcgcgtgt tcagcatcga taaacgcaca ggttttacct tcacgctgcg ctgcggcaat
    26461 cacctgcaac gtcagcgtgg ttttaccgga agattccggt ccgtagattt cgacgatacg
    26521 gcccatcggc agaccacctg ccccaagcgc gatatccagt gaaagcgaac cggtagagat
    26581 ggtttccaca tccatggaac ggtcttcacc caggcgcatg atggagcctt taccaaattg
    26641 tttctcaatc tggcccagtg ctgccgccaa cgctttctgt ttgttttcgt cgatagccat
    26701 ttttactcct gtcatgccgg gtaataccgg atagtcaata tgttctgttg aagcaattat
    26761 actgtatgct catacagtat caagtgtttt gtagaaattg ttgccacaag gtctgcaatg
    26821 catacgcagt agcctgacga cgcaccgcat cacggtcgcc gctgaagcat tcccgcaggg
    26881 taatgccttc accgcgggca gtggcaaaag caaaccagac ggtgccgaca ggcttctctt
    26941 cactgccgcc atccggcccg gcgataccac taatagacac ggcataatca gcacgagccg
    27001 ctttcagtgc acctatcgcc atttccacca cgacgggttc actcaccgcg ccatgctgcg
    27061 ccagcgtctc ttcgcgtacg ccgatcattt gcgctttggc ttcgttactg taggtgacaa
    27121 atccgcgttc aaaccaggcg gaactgccgg caatatcggt aatcactttc gctacccaac
    27181 cgccggtaca agactcagca gttgttacgg ttgcgccacg ggctttcagc gcctgcccaa
    27241 cctgttcgct taactgcatc agttcactgt cagtcatcac aacctctgtc agtcaaaaat
    27301 ttatgccgga caagatagca ctttcgtcag gaagatggtg ttggcgttgc aattttacga
    27361 ggaggattca gaaaaaagct gattagccag agggaagctc acgcccccct cttgcaaata
    27421 gttactgcac gcgcgccagc gccacggctt gtcctaactg ccagaccgcc attgcgtaat
    27481 gggtactgtg gttataacgg gtaatggtgt agaagttcgg cagaccgtac cagtactggt
    27541 aacctgttcc aacatccaga cgcagcaggc tggcttgttg atggttgccc agcggctgct
    27601 gtggcgttaa acctgccgcg gcaagctgcg aaatgctgta cctggttttg aagccatttg
    27661 gcaagcctgg agcctgaccg tttgccatta ccgcgacctg atcgcctttc acccagccgt
    27721 gcgctttgaa ataattcgcc acgctaccga tcgcatcaac cggatcccac aggttgatat
    27781 gcccgtcgcc gctgaaatct accgcatatt gtttgtaaga cgacggcata aactgtccgt
    27841 agcccatcgc cccggcaaag gaacctttca gattgagcgg atcgtcctgc tcgtcgcgcg
    27901 ccatcagcag gaaggtttcc agctcgccag agaaatactc cgcgcggcgt gggtagttaa
    27961 atgacagcgt tgccagcgca tcgaggatgc gagttttccc catcacgcgc ccccagcggg
    28021 tttcaacgcc gataatcccg acgataattt ccggcggtac gccatacacc tgccacgcgc
    28081 gattcaacgc atcttcatac tgattccaga aaaccacacc gttctgcaca ttgtccggcg
    28141 taataaattt tttgcgataa cgcagccatg cgccgttcgg acctgatggc ggtttcaccg
    28201 atgtggttgg tgcctggtta tccatcagcc gcagtaccga atccagacgt ttcgcctggg
    28261 agagaatttc ctgcaactgc tgacgatcga aaccgtgttt gttcaccatt ctgtcgatga
    28321 actgctgggc attcgggtta ttagcgaaat cgccgcccat ctgcatcaca ttgtgctgcg
    28381 gctcaagcag gaagccgcca gacggcgttc cggtggtcgt ttcagtctca gtaggttttg
    28441 gcttgctgct acaggcggca agcaacacaa aaaggggaag caatgttaca taacgacgct
    28501 tgaacatgag gggtccattt aacagattca accaggggca agtatggtaa agcatcacgc
    28561 cccgcacaag gaagcggtag tcactgcccg atacggactt tacataactc aactcattct
    28621 cctcgctatc cttttactca aactttcaaa ttaaaatatt tatctttcat tttgcgatca
    28681 aaataacact ttaaatcttt caatctgatt agattaggtt gccgtttggt aataaaacaa
    28741 taaatcctga aggagagaac aatgatagaa accattactc atggtgcaga gtggtttatc
    28801 gggctgttcc aaaagggcgg agaggtgttt accgggatgg tgaccggcat tcttccgctg
    28861 ttgattagcc tgctggttat catgaacgca ctgattaatt ttatcggtca gcatcgtatt
    28921 gaacgttttg ctcaacgttg cgccggtaac cctgtttccc gttacctact gttaccgtgc
    28981 attggcacgt ttgtcttttg caatccgatg accttaagcc tgggtcgctt tatgccggaa
    29041 aagtacaaac ccagctacta cgcggcggcc tcttatagct gccactcaat gaatggcctc
    29101 ttcccccata tcaaccctgg cgaactgttt gttatcttgg cattgccagc ggtctgacaa
    29161 cgctgaacct gccacttggc ccactggcgg tgagctatct gctggttggt ctggtcacca
    29221 acttcttccg cggctgggtg accgatctga ccaccgctat ttttgagaaa aagatgggca
    29281 ttcaacttga acaaaaagtt catctggcag gagcaacatc atgacgcata ttcggatcga
    29341 aaaaggaacg gggggctggg gcggcccgct tgagctggaa accacgccgg gcaaaaaaat
    29401 cgtctatatc accccgccgg tacccggcct gcgattgtcg acaaactggc acagcttact
    29461 ggctggcagg ctattgacgg atttaaagaa ggtgaacccg cggaggcgga aattggtgtc
    29521 gcggtaatcg actgtggcgg cacattacgc tgcggcattt atccgaaacg gcgtattccc
    29581 accattaata tccactcgac gggcaagtcc ggtccactgg cgcagtacat tgtggaagat
    29641 atttatgtct ctggcgtaaa agaagaaaac atcactgtag tgggtgatgc gacaccacaa
    29701 ccctcttccg tgggccgtga ctatgacacc agcaagaaaa tcaccgaaca aagcgatggt
    29761 ttactggcga aggtgggaat gggtatgggt tctgccgttg ccgtgttgtt tcaatctggt
    29821 cgtgacacca tcgacactgt attaaaaacc attcttccgt ttatggcatt cgtctcggcg
    29881 ctcattggca tcattatggc ttctggcctt ggtgactgga ttgcccacgg ccttgctccg
    29941 ctggcgagcc atccactggg tctggtcatg ctggcgctca tctgctcctt cccgctgctt
    30001 tcacctttcc tcggcccagg cgcagttatc gcacaggtta tcggcgtatt gattggcgtg
    30061 cagattggtc tcggcaatat tccgccgcat ctggctttac ctgcactgtt tgccatcaac
    30121 gcgcaggcgg cctgcgactt cattccggtc ggtttgtcgc tggcggaagc ccgtcaggac
    30181 acggttcgcg tcggtgtccc ttctgtactg gtgagccgct ttttaaccgg cgcgccgact
    30241 gtactgatcg cctggtttgt ctccggtttt atctatcaat agaggctgaa acatgaccgt
    30301 tatttatcag accaccatca cccgtatcgg cgcgagtgcc actgacgccc tcagcgacca
    30361 gatgctcatc acctttcgtg aaggcgcgcc agcggacctc gaagagtatt gcttcattca
    30421 ttgccacggc gagttgaaag gtgcactcca tcccggtttg caatttacgc tcgggcagca
    30481 tcgctatccg gtgaccgctg ttggcagcgt ggcggaagac aaccttcgcg aactgggtca
    30541 tgtcaccctg cgcttcgatg gtttaaacga agcggaattt ccgggcactg tccatgtggc
    30601 aggccctgta cccgacgata tcgcgccggg atcggttttg aagtttgaat ctgttaagga
    30661 gtaaaaaatg aatcaggttg ccgttgtcat cggtggtggg caaaccttag gcgcgttcct
    30721 gtgccacggt ctggctgccg aggggtatcg cgtcgcggtt gtcgatattc agagcgacaa
    30781 agccgcaaat gtggcacaag aaattaacgc cgaatatggt gaaggaacag cgtatggttt
    30841 tggtgctgac gccactagcg agcaaagcgt tctggcgctc tctcgtgggg tagatgaaat
    30901 ctttgttcgt gtggatttac tggtctacag cgccggaatt gcaaaagcag cctttatcag
    30961 cgacttccag ctcggcgatt ttgaccgttc gctacaggtg aatctggtgg gttatttcct
    31021 gtgtgcgcgt gaattttcgc gtttgatgat ccgcgatggg attcagggac gcattattca
    31081 gatcaactcg aaatccggca aagtgggcag caaacacaat tctggttata gcgccgcgaa
    31141 atttggtggc gtagggctga ctcaatcact ggcgctggat ctggcggagt acggcattac
    31201 ggtgcattca ctgatgctcg gtaacctgct gaaatcgccg atgttccagt cgctgttgcc
    31261 acaatatgcc accaagctgg gtatcaaacc ggatcaagtc gagcagtatt acatcgacaa
    31321 agtaccgctc aaacgcggct gcgattatca ggatgtgctg aatatgctgc tgttctacgc
    31381 cagtcctaag gcgtcgtact gtaccggaca gtcgatcaat gtcaccggcg gtcaggtgat
    31441 gttctgatca acagcggaga tccgttaagg atctccgtga gactaataga atgcctgatg
    31501 cgctacgctc atcaggcata caggacttcc gccactacat taaggaaaag ttatggtatc
    31561 cgcactcatc actgtcgccg ttatcgcctg gtgtgcgcaa ctggccttag gcggctggca
    31621 aatttctcgt tttaaccgtg ccttcgacac actatgccag caagggcggg ttggcgtggg
    31681 ccgttcccgc gggcgcttta aaccgcgggt cgtggtcgcc atcgccctgg acgatcagca
    31741 gcgcatcgcc gacaccttgt ttatgaaagg actgaccgtc ttcgcccgac cgcaaaaaat
    31801 tcccgcaatt accggtatgc atgcgggtga tttacagccc gatgtgatct ttccccatga
    31861 tccactatca cagaatgctc tatcattggc gcttaaactg aaacgtggat aatttcgttg
    31921 tgaatgttac ttgcttgcga agttatcatt ttgaaaccta aatcaggtaa tcacgcccat
    31981 gaaacctcgt cagcgtcaag ccgccattct ggagtatctg caaaagcagg gaaaatgctc
    32041 ggttgaagaa ttggcgcaat attttgacac cacaggcaca actattcgca aagatctggt
    32101 cattctggaa catgccggaa ccgtcattcg tacttatggc ggagtggtgt tgaataaaga
    32161 ggaatccgat ccgcctatcg atcataaaac actcatcaac acccacaaga aagagctgat
    32221 agcagaagct gccgttggtt ttattcatga tggcgattcg atcattcttg atgctggcag
    32281 taccgttttg cagatggtgc ctctgctctc gcgctttaat aacatcacgg tgatgaccaa
    32341 cagcctgcat atcgtcaatg cgttatctga actggataat gaacaaacta tcctgatgcc
    32401 aggcggaacg tttcgtaaaa aatcggcctc atttcacggg cagctggcag agaatgcctt
    32461 cgagcatttc accttcgaca aattattcat gggtaccgac ggcatcgatc tcaatgcggg
    32521 cgtaaccacc ttcaacgagg tctataccgt cagtaaggca atgtgcaatg ccgcgcgcga
    32581 agtgattttg atggcggact catcaaagtt tggccgtaaa agccccaacg tagtttgcag
    32641 tcttgaaagc gtcgataagc tgattaccga cgcagatatc gatccggcgt ttcgtcaggc
    32701 gctggaagag aaagggatcg atgtgatcat aaccggagag agcaatgagt gaagcactac
    32761 tgaacgcggg acgtcagacg ttaatgctgg aattgcagga agcaagccgt ttaccggaac
    32821 gtctgggcga tgattttgtt cgcgccgcca atatcatcct gcactgcgaa ggcaaagtgg
    32881 tggtttcggg aattggcaaa tcgggccaca ttggtaagaa aatcgccgca acgcttgcca
    32941 gtaccggcac accggctttt tttgtccatc cggcagaagc gctgcatggc gatctgggga
    33001 tgatcgaaag ccgcgatgtg atgctgttta tctcttactc cggtggggcg aaggaactgg
    33061 atctgattat tccgcgtctg gaagacaaat ctatcgcgct gctggcgatg accggcaaac
    33121 cgacgtcacc gctgggcctg gcggcgaaag cggtgctgga tatctccgta gaacgcgaag
    33181 cctgcccgat gcaccttgcg ccgacctcca gcaccgtcaa taccctgatg atgggtgacg
    33241 cgctggcgat ggcggtcatg caggcgcgcg gatttaatga agaagatttt gcccgctccc
    33301 accctgctgg agcactgggc gctcgcttgc tgaataaagt gcatcatctg atgcgccgtg
    33361 acgatgccat cccacaggtg gcgttaaccg ccagcgtgat ggatgctatg ctggaactca
    33421 gccgcaccgg tctggggctg gtggcggtat gtgacgctca acaacaggta caaggcgtct
    33481 ttaccgacgg cgatttacgt cgctggctgg ttggcggcgg cgcactcacc acgccagtca
    33541 atgaagcgat gacggtcggc ggcaccacgt tgcaatcgca aagtcgcgcc atcgacgcca
    33601 aagagatcct gatgaagcgc aaaatcactg ccgcaccggt ggtggatgaa aacggcaaac
    33661 tcaccggcgc aataaacctg caggatttct atcaggccgg gattatttaa tccttcaatc
    33721 ccagacgttt cgccagccga tgcaggttgg cgacgtcggt ttccagcatc cgcgcgcagg
    33781 cagcccagtt gtgatgattt tgtgccagtg cctgacgaat agtttcacgc tggaacgctt
    33841 ctgtcgcttc acgcaggttt tgcttaacaa cgggcaccgc cgccacttct ggcggcggca
    33901 acgtcacctc aggaaaagca aaatgttgcg cctcaagaat cacttcatcg ccgctgcggg
    33961 tggctctcgc cagcactacc gcccgatgaa tagcatgttc cagttcgcgc acgtttcccg
    34021 gaaaactgta gtgttgcagt aaatttcgcg ccccggcact taataccacg cgggagagcc
    34081 cctgccgcaa acgacactgc tcgcagaaat accccgccag cagaatgaca tcatcgcccc
    34141 gctcacgcag cggcggcacc gaaagtggaa acacactcag gcgatgaaac aaatcggcgc
    34201 ggaatcgccc tgccagcacc tcttcgcgta agtcgcggtt agtcgccgcc agcacgcgca
    34261 catcgacccg caaactacgg tcatcgccaa cgcgctgaat atcgccatac tgcaacaccc
    34321 tcagcagctt ggcctgcaat gccaacgaca actcgccgat ctcatcgaga aacagcgtgc
    34381 cgttatccgc catttcaaac ttcccactgc ggttactgat agcgccagta aacgctcctt
    34441 tcacatgccc gaacaactca ctttccgcca cactttccgg cagtgcggca cagttgagat
    34501 agaccagcgg attcaccgcc cgtggcgagg cctcatgaat cgctttcgcc accagctcct
    34561 taccggttcc cgtctcaccg ctgatcagga cgttgagatc ggacgccgcc acaatctcaa
    34621 tctctttttt cagttgcgtc atgcctggcg acagaccgat catctgcgtc tgtttcaccg
    34681 cttcaaatgg agcggcatcg cccggcagca tattctggct ttccagttgt tcaatcagca
    34741 acgcattgct taacgctccc gccgccagcg cggcaatcag gcgtaactct tcgtcgctga
    34801 aaacatcgaa ctgatcgggc tgcatcccgt cgagcgtcaa tgcgccgatc aggttttgcc
    34861 cggcaaacag cggcagacca acgcaggcgt gaaccttcag actctcctgc cctggaatca
    34921 aaccgtcata gggatcgggc aattcgctgt ctgcgggaaa gcgcaccaca tcacccgcgc
    34981 gggcaatcgc ttccagccgt ggatgacctt ccagcgcaaa gcgtctaccg agtacatcct
    35041 tcgccagccc gtcgatggca agcggaataa actgccgcga gtcgtaacgt agcaacgcag
    35101 acgcatcgca ctccagcacc tgacgcagcg tggtgatcag gcgctgaaaa cgatcctgat
    35161 gaccaatccc acgctgcaat tcgatggcga tattcgccag cacatcaacg gaaaaactca
    35221 tctttgcctc actgtcaatt tgactataga tattgtcata tcgaccattg gattgatagt
    35281 cattttgact actcattaat gagcataatt ttatttatag agtaaaaaca atcagataaa
    35341 aaactggcac gcaatctgca attagcaaga catcttttta gaacacgctg aataaattga
    35401 ggttgctatg tctattgtgg tgaaaaataa cattcattgg gttggtcaac gtgactggga
    35461 agtacgagat tttcacggca cggaatataa aacgctgcgc ggcagcagct acaacagcta
    35521 cctcatccgc gaagaaaaaa acgtgctgat cgacaccgtc gaccataaat tcagccgcga
    35581 atttgtgcag aacctgcgta atgaaatcga tctggcggat atcgattaca tcgtgattaa
    35641 ccatgcagaa gaggaccacg ccggggcgct gaccgaactg atggcacaaa ttcccgatac
    35701 gccgatctac tgtacagcca acgctatcga ctcgataaat ggtcatcacc atcatccgga
    35761 gtggaatttt aatgtggtga aaactggcga cacgctggat atcggcaacg gcaaacagct
    35821 catttttgtc gaaacaccaa tgctgcactg gccggacagc atgatgactt acctgacagg
    35881 cgacgcggtg ctgttcagta acgatgcttt cggtcagcac tactgcgacg agcatctgtt
    35941 caacgatgaa gtggatcaga cggagctttt tgagcagtgc cagcgttact acgccaatat
    36001 ccttacgccg ttcagccgtc tggtaacgcc gaaaattacc gagatcctgg gctttaactt
    36061 accagtcgat atgatagcga cctctcacgg cgtggtatgg cgcgataacc cgacgcaaat
    36121 tgtcgagctg tacctgaaat gggcggctga ttatcaggaa gacagaatca ccattttcta
    36181 cgacaccatg tcgaataaca cccgcatgat ggctgacgct atcgcccagg ggattgcgga
    36241 aaccgaccca cgcgtggcgg tgaaaatttt caacgtcgcc cgaagcgata aaaacgaaat
    36301 cctgaccaat gtcttccgct caaaaggcgt gctggtcggc acttcgacga tgaataacgt
    36361 gatgatgccg aaaatcgccg ggctggtgga ggagatgacc ggattacgct tccgtaacaa
    36421 acgcgccagt gctttcggct ctcacggctg gagcggcggt gcggtggatc gtctttccac
    36481 gcgcctgcag gatgcgggtt tcgaaatgtc gcttagcctg aaagcgaaat ggcgaccaga
    36541 ccaggacgct ctggagttat gccgtgaaca cggtcgcgaa atcgcccgtc agtgggcgct
    36601 cgcgccgctg ccgcagagca cggtgaatac ggtagttaaa gaagaaacct ctgccaccac
    36661 gacggctgac ctcggcccac ggatgcagtg cagcgtctgc cagtggattt acgatccggc
    36721 aaaaggcgag ccaatgcagg acgttgcgcc aggaacgccg tggagtgaag tcccggataa
    36781 cttcctgtgc ccggaatgct ccctcggcaa agacgtcttt gacgaactgg catcggaggc
    36841 aaaatgagca acggcattgt gatcattggt tcgggcttcg ccgcccgcca actggtgaaa
    36901 aatattcgca aacaggacgc cagtattcca ttaaccctga ttgccgccga cagtatggat
    36961 gagtacaaca aacctgacct cagccatgtt atcagtcagg ggcaacgtgc cgacgacctt
    37021 acccgccaga cggcgggtga atttgccgag cagtttaatc tgctcctgtt tccgcaaacc
    37081 tgggtgacgg atatcgatgc cgaagcccgt gtggtgaaaa gccagaataa tcagtggcaa
    37141 tacgacaagc tggtgctggc aaccggtgcc agcgcctttg tcccgccagt gcctgggcgt
    37201 gagttaatgc tgacgttaaa tagtcagcaa gagtatcgcg cctgtgaaac gcaactgcgg
    37261 gatgcccgac gcgtgttgat tgttggcggt ggtttgattg gtagcgaact ggcgatggat
    37321 ttttgtcgtg caggcaaaat ggttacgcta atcgacaacg ctgccagtat tctggcgtcg
    37381 ttaatgccac cggaagtaag cagtcgcttg cagcatcggt tgacggagat gggcgttcat
    37441 ctgctgttga aatctcagtt gcaggggctg gaaaaaacgg attctggcat tctggcaacg
    37501 ctggaccacc agcgcagcat cgaagtggat gcggtaattg ccgccaccgg actgcgcccg
    37561 gaaaccgccc tggcacgacg cgccgggctg acgattaatc gcggcgtttg cgtcgatagt
    37621 tatctgcaaa ccagtaatgc cgatatttac gcgctgggcg attgcgcgga aattaacggt
    37681 caggtattgc cgttcctgca gccgattcaa cttagcgcga tggtgctggc aaaaaatctt
    37741 ctcggcaata acacgccgct gaaactaccg gcgatgctgg tgaaaatcaa aacgccggaa
    37801 ttaccgctgc atctggcagg cgaaacccag cgtcaggatt tacgctggca gattaatacc
    37861 gaacgccagg gaatggtggc gcgcggtgtt gacgatgcag accaacttcg cgcctttgtg
    37921 gtcagtgagg atcggatgaa agaggcattt ggattgttga aaacgttgcc ggtgtaggtg
    37981 ggctactgtg cctaaaatgt cggatgcgac gctggcgcgt cttatccgac ctacggggac
    38041 gcatgtgtag gccggataag gcgtttacgc cgcatccggc aatggtgtcc aaatgcaaca
    38101 cgttttatcc gttctggact tcacccgcta accaacgcgc cgcagcaata accccctgcc
    38161 ccagagacaa accgccatca cccgccggta aactctgtgg aaacagcaat gtgaaatcag
    38221 cgagataatg cgccagacgt gcacgcagca aacggttatg aataaccccg ccgctaaata
    38281 ccagcgtagt gataccacgc atcgtggcct gctcacgcat caacgcggca aaaccctgcg
    38341 ccagcgcatc atgaaacgcc cacgcgcgtt gattaaccgg tgcctgccag ttcagccact
    38401 gctgccagaa agtggcgaga tccagttgat tgtccacccg cggcatcgtc accggatgcg
    38461 tcactccgtc gcatgaggct gctagcgcct ccagagcaca agccgcttca ccttcataac
    38521 ttaacgtggc tggcgcacag cccagtgccg ccgccacagc atcgaacaaa cgcccacacg
    38581 atgacgccag cggcgcgtta attccacgct caatggcccg cgccagtacg ctccagtttt
    38641 gctgttgcac acttgccgtt tcagggtaat tctgccactc cggcacaaag cgcaggcact
    38701 gcgccagcag gttacgccac ggctgcttcg ccgccaaatc gccacccgga agcgccactg
    38761 caggcaagcc gcccaggtgc tcacattcgc gatagttcac ccgcaggcac tcgccgcccc
    38821 acaaagcgcc gttctccccc ataccgatac cgtcgagcgt caaagcaatg acatcaccgc
    38881 catccagcgg ccactgatgc tctgccagac acgccgctgc gtgggcatga tgatgcagca
    38941 ccgtttgcgt cggcagattc atttcgctcg cccactggca ggagacatag cccggatgtg
    39001 cgtcatgcac aacgtattgc ggagtgaaat tgtagatgtt ttgcatcagg cgtaacgctt
    39061 cgcgccactg cgtctggatg ccatcgtcac ttaaatcgcc cagatgctgg ctcaacacca
    39121 cttgttcacc gcgcaccagg cagaaggtat ttttcagatc cgcgccgaga cacagtacag
    39181 gcggaacatt tttaaagccc ggaggcaaag ccagcgcatc cggcacatac ccccgcgaac
    39241 ggcgcagcat ttcgccgctt tcgcgcacca ccgaatcatc catccgctgc acaatgtcgc
    39301 ggttgtgtat taagaatccg tcggcaatgc cctgcaaatc ctccagcgcc tgttcgttgc
    39361 tgatagccgg tggtttaccg ctcaggttgc cagaggtcat caccagcggg cattgtagtt
    39421 cctgtaacag caaatgctga agcggattcg caggcaacat taccccgact tcgttaaggc
    39481 caggggcgat atcatcacaa agctcaggaa cgtatttttt atccaccagc acaatcggcg
    39541 cggcgggcgt ggtaagcaac tggcgcgcag cgtctggtaa accgtcagcc actggcaaca
    39601 tgactgccag cggtttcgcc gggcgatgtt tgcgcgcccg aagtgtcgcc accgcgttac
    39661 tgttacgtgc atcgcaggca agatgaaatc cgccaatccc tttgatggcg acaatgttgc
    39721 ccatttttaa ctgtgcgata gccgcctgta atgccgcctc ttgttctgca tgttcaccat
    39781 gacttaccca ttcaagatac gggccacact ccgggcaggc caccggctgg gcgtggaagc
    39841 gacgatcgag cgggtcgcgg tactctttat cacaggctgg acacagcgga aacgccgcca
    39901 tcacggtaaa cgggcgatcg taaggcatgg cgcgaataat ggtgaagcgc gggccgcagt
    39961 gggtacagtt gataaatgga taacgataac gccgttcgcc tggggtattc atttcggcaa
    40021 ggcaagcagg gcaagtggcg gcatcgggaa caatttgcgt attcatggcg ccgcccgcgc
    40081 tctggcggat ggtgaactca gtgggcagtt gtgaccagat aaacggttca cgctcaacgc
    40141 tatcaatacg cgccagcggc gggcagtgct gatgcaattg aacaagaaac gtttccgggt
    40201 cttccagcag ccggacttct acgccatcgc cgtcattaca gacatcgccg tgaagattta
    40261 attgctgtgc cagctgccag acaaacggac gaaaaccgac gccctgcact ttgcctcgaa
    40321 tacgcagttg gacaccgcaa gatgtgtttt ttgccattga gttattcccg ccatcatgaa
    40381 ttgcgtaacc cgtccagccg gacacgacag cgtcgcatcc ggcagtcaca ggtcggcgat
    40441 accgcccgct ccgtattcta cgaatatttc cgggaattcc tttgatgcca gaacagttct
    40501 gtaagatttt tagaacatca gcgccgtacg gcggcgtttt tctgcgctca gttgttcaag
    40561 tttattgcga tcgacacaaa ttagcgcatg ggtcgggcaa gccgccatac acgccgggcc
    40621 gtcttcacga tggttgcaca ggtcgcattt attggcttcg gctttgtcag cccgtacatt
    40681 cagccctgcg ccgctgttgc gaatcaccgg acgtaccacc acttccatcg cgccatacgg
    40741 gcaagccaca acgcaggttt tgcaaccaat gcaacgttcc tgcatcacat gaacaaaccc
    40801 tttatcacgg ctgatagcac cattcgggca gacgttagcg cacggtgcat cttcacactg
    40861 acggcaaact gtcgccgtgg aaatgttcac acctttaatg acatggatac gcggtaaaaa
    40921 tgtttccggg gtcagcgatg cacagtcctg attttcctga tgagaaacca cgcacgccac
    40981 ttcacaggta cggcaaccaa tacatttact cgcgtcagca atgatgaaac ggttcatcaa
    41041 attctccagc aatgacagtt aatgcgccga tacattcaca aatcatgcca gtttttaatt
    41101 tgctgttatt taaggaaatt aatttctgta atgcaggaaa aaagatgtca tcgacactag
    41161 tgacgatgac atgtgatgac aatgtttatc gcgaaagagc aatgagtgag tcgcggcgga
    41221 tcagttttcc gctgaaggtt ttcggcggtg agaaatcccc gccatcgagc ataaaaatca
    41281 gccgtccaat aatttcctga atcatctcag tcaccggaat ttttacgctg gagagcgccg
    41341 gaacgatata gggggcaatt gcgatatcat cgaatccgat aactgacacc tgctctggca
    41401 ccgctacgcc gcgcttgtgt aacgctttca tcgcacctat cgccatatcg tcgttactgg
    41461 caactaacgc gctaaattta gccccacgtt cgagcaacgt ttctacccct tcggccccgc
    41521 tggcgggcgt ccatttaccg ttagcgataa gtttttcatt gagcgcaata ccatgctgcg
    41581 ccagcgcgtc tttatatccg gcaagacgtt caacgctggt gggggaatcc atcgagccgg
    41641 taagaaaagc aatctcctga tgcccggcgt ttatcaactc tgccacggcg ttaaagctgg
    41701 tctgtttatg atcgcaccag acgctatggc tgctattttt gcgcaggcgg cgattaagca
    41761 ccattatcgg ctgactgtgc gcgtcaatga tgtcatcgat ctcatccaca cttaaaaaac
    41821 gcgggtaaat cataatggcg tcgcagcgca aatccagcag atactgaatc gcctggcgct
    41881 cttcttcggc gctgtgttta ccatctgcca atagcaactg ccgccctttc tcttccgcca
    41941 ttcgcgcggc atgaaacagt aattcactaa aataaatgcc gtggtaaagc gtgttagtca
    42001 ctaccagccc cagcgtctgg gtacttttcg ccgacagatt gcgcgccagc aagtttggac
    42061 ggtaaccgct ctcttctacc gcctgaaaca cgcgatcttt agtctcctgg ctgacgtagc
    42121 cattacctga aagcacgcgg gaaacggtcg cttttgaaac cccggcgcgc ttcgccactt
    42181 ccagcatcgt cgtcatcatt ttcatccctt tacacgcaat caacgcagtg tactgcaccg
    42241 tttaccgatt gtccttgcgc aatcggcggg aaaaatattc aggtgaccgg tttcacaaat
    42301 ataaaaaatg aacaattaac tctcttgctt atttggttac aactattcat gattttgtga
    42361 aaccggtttc ttaattccgt ttcagcatcg gcatttttcc gtcacgtcga ctgataacaa
    42421 ctacatcttc cctactgata acaggataaa atccgatggc caaaaattat gcggcgctgg
    42481 cacgctcggt gatagcggca ctgggcggcg ttgataacat ctcggcggtc acgcactgta
    42541 tgacgcgttt gcgctttgtt atcaaagatg atgcgcttat cgacagcccg acgttaaaaa
    42601 ccatccccgg cgtg
//
LOCUS       DAZFAB010000029        42125 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZFAB010000029 DAZFAB010000000
VERSION     DAZFAB010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42125
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.029"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            220..753
                     /locus_tag="ACHJWA_001862"
     CDS             220..753
                     /locus_tag="ACHJWA_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4308472.1"
                     /translation="MKKYIIPFVATTLMFSATTFAKDVDQGTLTIRGEVVDTTCYFVN
                     GDSTTDIVVEDVVTSAMNQLINNEIYTLKTGSTSHDLEIQCDGNTAPSIEVLASEFDA
                     NNFTLNKGTAENIGFAIFLDDGTLTRMRPGLKTPLKDNGSGQYFLNLNAQYARTSGNP
                     VTKGSVESTVTIKISAD"
     gene            803..1486
                     /locus_tag="ACHJWA_001863"
     CDS             803..1486
                     /locus_tag="ACHJWA_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO4308473.1"
                     /translation="MRIITALLMSFFILPVHAGVVIYGTRIIYPEKNREVLVQLMNQS
                     KNASLIQAWIDDGNTTIAPEKIQVTFILTPPVSRVAGGSGQQLKIRKMPNNLPHNKES
                     LFYLNVLDIPPNNPQNAGKNKIKLALQNRIKLLWRPSGIAPVDKKSLSQLNIKKKNNA
                     ISINNETANWITVTTIKAQNVKVNNESILLPPFSHNDITLKNNHASEYELTVVDDYGN
                     NIHSKIAAR"
     gene            1499..3949
                     /locus_tag="ACHJWA_001864"
     CDS             1499..3949
                     /locus_tag="ACHJWA_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFO4308474.1"
                     /translation="MLRTTRWVAAIIFLYSFPGYAEETFDTHFMIGGMKGEKVSEYHF
                     DNKQPLPGNYELDFYVNNQWRGKQDITIPESPVKPCLPKVLLTKLGVKTGNLNTEDNC
                     ILLDKAVHGGQYQWDISEHRLNLTVPQAYINELERGYVPPESWDRGIDAFYTSYNLSQ
                     YRSYDSNNNSNTASYGRFNSGLNLFSWQLHSDASYSKPDDMKGTWQSNTLYLEHGWSQ
                     ILSTVQIGENYTSSLIFDSLRFSGIRLFRDMQMLPDSMQSFTPLVQGVAQSNALITVS
                     QNGYIIYQKEVPPGPFTIADLQLSGSGSDLDVSIKEADGSVRSFLVPYSSVPNMLQPG
                     ISNFDFIAGRSKIYGVKNQEDFLEANYIYGLNNLLTLYGGTILSDNYNAITLGNGWNT
                     PLGAISFDATRSSSKLNNDITHEGTSYQVAYNKYLVQTATRFSVAAWRYASQDYRTFS
                     DHLYENDKINHQSDYDDFYDIGRKNSLSANIMQPLSNNLGNVSLSALWRNYWGRSGNA
                     KDYQFSYSNSWQRISYTFSASQSYDENDKEEERFNLFISIPFYWGDDIAKTRHQINLS
                     NSTSFSKDGYSSNNTGITGIAGEHDQLNYGIYVNQQQQNNDTSLGTNLSWRTPIATID
                     GSYSHSKNAWQSGGSISSGLVVWPGGINITNQLSDTFAILDAPGLEGAHINGQKYNRT
                     NSKGQVVYDLMIPHRENHLVLDTANSESETELQGNRQIIAPYRGAVSYVQFTTDQRKP
                     WYIQALRPDGSPLTFGYDVLDLQENNIGVVGQGSRLFIRVDEIPTGIKVALNDEQNLF
                     CTITFQHVIDENKTYICQ"
     gene            3962..4922
                     /locus_tag="ACHJWA_001865"
                     /pseudo
     CDS             3962..4922
                     /locus_tag="ACHJWA_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
     gene            complement(4960..5859)
                     /gene="nhaR"
                     /locus_tag="ACHJWA_001866"
     CDS             complement(4960..5859)
                     /gene="nhaR"
                     /locus_tag="ACHJWA_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFO4308475.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADV
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(5925..7091)
                     /gene="nhaA"
                     /locus_tag="ACHJWA_001867"
     CDS             complement(5925..7091)
                     /gene="nhaA"
                     /locus_tag="ACHJWA_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFO4308476.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            7677..7829
                     /gene="mokC"
                     /locus_tag="ACHJWA_001868"
     CDS             7677..7829
                     /gene="mokC"
                     /locus_tag="ACHJWA_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFO4308477.1"
                     /translation="MKQHKVMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(7933..9063)
                     /gene="dnaJ"
                     /locus_tag="ACHJWA_001869"
     CDS             complement(7933..9063)
                     /gene="dnaJ"
                     /locus_tag="ACHJWA_001869"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFO4308478.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(9152..11068)
                     /gene="dnaK"
                     /locus_tag="ACHJWA_001870"
     CDS             complement(9152..11068)
                     /gene="dnaK"
                     /locus_tag="ACHJWA_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFO4308479.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            11445..11849
                     /gene="yaaI"
                     /locus_tag="ACHJWA_001871"
     CDS             11445..11849
                     /gene="yaaI"
                     /locus_tag="ACHJWA_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFO4308480.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNALAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            11875..12588
                     /gene="msyB"
                     /locus_tag="ACHJWA_001872"
     CDS             11875..12588
                     /gene="msyB"
                     /locus_tag="ACHJWA_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFO4308481.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFVGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSSYRVT
                     IPAVLQIACLRQMVSATQV"
     gene            12737..13303
                     /gene="satP"
                     /locus_tag="ACHJWA_001873"
     CDS             12737..13303
                     /gene="satP"
                     /locus_tag="ACHJWA_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFO4308482.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(13338..13925)
                     /gene="mog"
                     /locus_tag="ACHJWA_001874"
     CDS             complement(13338..13925)
                     /gene="mog"
                     /locus_tag="ACHJWA_001874"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFO4308483.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(14040..14993)
                     /gene="tal"
                     /locus_tag="ACHJWA_001875"
     CDS             complement(14040..14993)
                     /gene="tal"
                     /locus_tag="ACHJWA_001875"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO4308484.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            15272..16702
                     /gene="yaaJ"
                     /locus_tag="ACHJWA_001876"
     CDS             15272..16702
                     /gene="yaaJ"
                     /locus_tag="ACHJWA_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFO4308485.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAIIRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVINIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSRDAWDDVSQE"
     gene            16772..17548
                     /gene="yaaA"
                     /locus_tag="ACHJWA_001877"
     CDS             16772..17548
                     /gene="yaaA"
                     /locus_tag="ACHJWA_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFO4308486.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(17701..17997)
                     /gene="yaaX"
                     /locus_tag="ACHJWA_001878"
     CDS             complement(17701..17997)
                     /gene="yaaX"
                     /locus_tag="ACHJWA_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFO4308487.1"
                     /translation="MKKMQSIVLALSLVLVAPMATQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAHHDHHGGHGPGKHHR"
     gene            complement(18211..19497)
                     /gene="thrC"
                     /locus_tag="ACHJWA_001879"
     CDS             complement(18211..19497)
                     /gene="thrC"
                     /locus_tag="ACHJWA_001879"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFO4308488.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(19498..20430)
                     /gene="thrB"
                     /locus_tag="ACHJWA_001880"
     CDS             complement(19498..20430)
                     /gene="thrB"
                     /locus_tag="ACHJWA_001880"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFO4308489.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVESAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(20432..22894)
                     /gene="thrA"
                     /locus_tag="ACHJWA_001881"
     CDS             complement(20432..22894)
                     /gene="thrA"
                     /locus_tag="ACHJWA_001881"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFO4308490.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(22920..23033)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(22975..23058)
                     /locus_tag="ACHJWA_001882"
     CDS             complement(22975..23058)
                     /locus_tag="ACHJWA_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFO4308491.1"
                     /translation="MKRISTTITTTITTTITITITTGNGAG"
     gene            complement(23272..23958)
                     /gene="yjtD"
                     /locus_tag="ACHJWA_001883"
     CDS             complement(23272..23958)
                     /gene="yjtD"
                     /locus_tag="ACHJWA_001883"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO4308492.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLAILIQ
                     QPTKSDTTADQHQLQALRERAMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(24358..24498)
                     /gene="yjjY"
                     /locus_tag="ACHJWA_001884"
     CDS             complement(24358..24498)
                     /gene="yjjY"
                     /locus_tag="ACHJWA_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFO4308493.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            24594..25310
                     /gene="arcA"
                     /locus_tag="ACHJWA_001885"
     CDS             24594..25310
                     /gene="arcA"
                     /locus_tag="ACHJWA_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFO4308494.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(25370..26722)
                     /gene="creD"
                     /locus_tag="ACHJWA_001886"
     CDS             complement(25370..26722)
                     /gene="creD"
                     /locus_tag="ACHJWA_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFO4308495.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITFGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGIGLEQGGQGVHIPLPEGDWRKQNLKQNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(26780..28204)
                     /gene="creC"
                     /locus_tag="ACHJWA_001887"
     CDS             complement(26780..28204)
                     /gene="creC"
                     /locus_tag="ACHJWA_001887"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFO4308496.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLEQALGNLLDNAIDFTPKSGRITLS
                     AEVDQEHVALKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(28204..28893)
                     /gene="creB"
                     /locus_tag="ACHJWA_001888"
     CDS             complement(28204..28893)
                     /gene="creB"
                     /locus_tag="ACHJWA_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFO4308497.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDIVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRSL"
     gene            complement(28906..29379)
                     /gene="creA"
                     /locus_tag="ACHJWA_001889"
     CDS             complement(28906..29379)
                     /gene="creA"
                     /locus_tag="ACHJWA_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFO4308498.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            29590..30459
                     /gene="robA"
                     /locus_tag="ACHJWA_001890"
     CDS             29590..30459
                     /gene="robA"
                     /locus_tag="ACHJWA_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFO4308499.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(30456..31103)
                     /gene="gpmB"
                     /locus_tag="ACHJWA_001891"
     CDS             complement(30456..31103)
                     /gene="gpmB"
                     /locus_tag="ACHJWA_001891"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFO4308500.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            31155..31667
                     /gene="yjjX"
                     /locus_tag="ACHJWA_001892"
     CDS             31155..31667
                     /gene="yjjX"
                     /locus_tag="ACHJWA_001892"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFO4308501.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASIAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTTQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVY"
     gene            complement(31814..32140)
                     /gene="trpR"
                     /locus_tag="ACHJWA_001893"
     CDS             complement(31814..32140)
                     /gene="trpR"
                     /locus_tag="ACHJWA_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFO4308502.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(32230..34167)
                     /gene="sltY"
                     /locus_tag="ACHJWA_001894"
     CDS             complement(32230..34167)
                     /gene="sltY"
                     /locus_tag="ACHJWA_001894"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFO4308503.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            34378..36045
                     /gene="ettA"
                     /locus_tag="ACHJWA_001895"
     CDS             34378..36045
                     /gene="ettA"
                     /locus_tag="ACHJWA_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFO4308504.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(36152..36319)
                     /gene="nadS"
                     /locus_tag="ACHJWA_001896"
     CDS             complement(36152..36319)
                     /gene="nadS"
                     /locus_tag="ACHJWA_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFO4308505.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVARHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(36353..37585)
                     /gene="nadR"
                     /locus_tag="ACHJWA_001897"
     CDS             complement(36353..37585)
                     /gene="nadR"
                     /locus_tag="ACHJWA_001897"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFO4308506.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVVDGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEEDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(37606..38988)
                     /gene="radA"
                     /locus_tag="ACHJWA_001898"
     CDS             complement(37606..38988)
                     /gene="radA"
                     /locus_tag="ACHJWA_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFO4308507.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(39037..40005)
                     /gene="serB"
                     /locus_tag="ACHJWA_001899"
     CDS             complement(39037..40005)
                     /gene="serB"
                     /locus_tag="ACHJWA_001899"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFO4308508.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            40111..40752
                     /gene="ytjB"
                     /locus_tag="ACHJWA_001900"
                     /pseudo
     CDS             40111..40752
                     /gene="ytjB"
                     /locus_tag="ACHJWA_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
     gene            40783..41799
                     /gene="lplA"
                     /locus_tag="ACHJWA_001901"
     CDS             40783..41799
                     /gene="lplA"
                     /locus_tag="ACHJWA_001901"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFO4308509.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGIPHEQVCEAITEAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            41831..42094
                     /locus_tag="ACHJWA_001902"
     CDS             41831..42094
                     /locus_tag="ACHJWA_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4308510.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
BASE COUNT        10523 a        11042 c        10168 g        10392 t
ORIGIN      
        1 gaagcatgct ctgaaacatc ctactctgtc actctaatga tggcaccttg ctcttcttat
       61 aaagaaggga ataattaaga atattaaatt ttcaaacttt catcaacttc acatcaagaa
      121 attaaaaaat ttaaacatta atctaaataa taacaacctg atcacataat cgggtttgaa
      181 aacgagtgac cttccatacc agttaattaa ggatgattta tgaaaaagta tattattccc
      241 ttcgttgcga caacgttgat gtttagcgca acaaccttcg ccaaggatgt cgatcaagga
      301 acactaacca ttagaggaga agttgttgat acaacgtgtt actttgttaa tggtgattcg
      361 acaacagata tcgttgtcga agatgttgta accagtgcaa tgaatcaact tatcaataat
      421 gaaatctata ctttaaaaac cggttcaacc tctcatgact tagaaattca gtgtgacgga
      481 aatacagccc caagtatcga agtactagca tcagagtttg atgctaataa ctttacgctg
      541 aataaaggaa ctgctgagaa cattggcttt gccattttcc tcgatgatgg gactttgacc
      601 agaatgagac cagggttaaa aacaccgctg aaagataatg gctctggtca atattttctc
      661 aatttaaatg cacaatatgc ccgcacaagt ggcaatcctg ttacaaaagg ctccgtagag
      721 tcaaccgtaa cgattaaaat cagtgcagat taacgttttt tgccggtagc tctgctaccg
      781 gtattattta cggagtgatt acgtgagaat aataacagca ttactgatgt cattttttat
      841 tttacctgtt catgccggtg tggtcatcta tggtacgcgc attatttatc cggaaaaaaa
      901 cagagaagtt ctcgtacagt taatgaatca aagtaaaaat gcctctttga ttcaggcatg
      961 gatcgatgat gggaacacca ctatcgcgcc agaaaaaatt caggtcacat ttattctcac
     1021 accgccagta agtcgtgtag caggtggttc tggccaacaa cttaaaataa gaaaaatgcc
     1081 aaacaatttg cctcacaata aagaaagttt attttatcta aatgttctgg atatccctcc
     1141 aaacaatcca caaaatgcag ggaaaaacaa aattaaactt gcattgcaaa accggataaa
     1201 gttactttgg cgtccaagcg gaatagctcc ggtagataaa aaaagccttt ctcaattaaa
     1261 tatcaaaaag aaaaataacg ccatctcaat aaacaacgaa acagccaact ggataacggt
     1321 tacaactatc aaagcacaaa acgtgaaagt taataatgaa agtatcttac tgcctccctt
     1381 ttcgcataac gacattacat taaaaaataa ccacgcatca gaatatgaac taacggtagt
     1441 tgatgattac gggaataaca tccatagtaa aatcgccgcg agataacagg aaaaagtcat
     1501 gttaaggacg acacgatggg tcgctgctat tatttttttg tattcttttc cgggttatgc
     1561 tgaagaaact tttgatacgc actttatgat cggcggcatg aaaggagaaa aagtttccga
     1621 atatcatttc gataataaac aaccgcttcc aggaaactac gaacttgatt tttatgtaaa
     1681 taaccagtgg cgtggaaaac aagatatcac gatcccagag tcaccagtca aaccatgtct
     1741 gcctaaggtg ctactaacca aactgggggt aaagaccggt aacctcaata cagaagataa
     1801 ttgtatttta ctggataaag cggttcatgg tggacaatat cagtgggata tcagtgaaca
     1861 ccgactaaat ttaacagtac cacaggcgta tatcaatgaa cttgaaagag gttacgttcc
     1921 cccggaaagt tgggatcgcg gcattgatgc tttttatacc tcctataatt taagtcagta
     1981 tcgctcttat gacagcaata ataacagtaa tacagccagt tatggccgat ttaacagtgg
     2041 attaaattta tttagttggc agttacactc tgatgccagc tatagcaaac ccgatgatat
     2101 gaaaggcaca tggcaaagta atactcttta cctggaacat ggctggtcgc agatattaag
     2161 cacagttcag ataggagaaa attacacatc ctcactcata ttcgactctc tgagatttag
     2221 cggaatacgc ttatttcgcg atatgcaaat gctacctgat tctatgcaga gctttacacc
     2281 tttagtacaa ggtgttgcgc aaagtaatgc cttgataacg gtttcgcaaa atggctacat
     2341 catttaccaa aaagaggttc ccccgggacc atttactatc gcagatttgc aactctcggg
     2401 cagcggctct gatctcgatg tcagcataaa ggaagcagat ggcagtgttc gttccttttt
     2461 ggtgccctac tcttccgtgc ctaacatgct gcaacctggt atttcaaatt tcgattttat
     2521 cgccggacgc agtaagatat atggtgtaaa gaatcaggag gactttctcg aggcaaatta
     2581 tatatatggc ctcaacaatc ttttgacact ttatggcggt acgattttat ccgataacta
     2641 taatgctata actctgggaa atggttggaa tacaccgctg ggtgccatct cttttgacgc
     2701 tacacgatcg agtagtaagt taaataatga tataacacac gaaggtacga gctatcaggt
     2761 cgcctataat aaatatctag tacaaacggc cactcgattt agcgtggccg cctggcgtta
     2821 tgcttcgcag gattacagga cattcagcga ccatctttac gaaaatgata aaataaatca
     2881 tcagagtgac tatgacgatt tttatgatat tggcagaaaa aatagccttt ctgccaatat
     2941 catgcaacct ttatccaata atctgggaaa tgtatcatta agtgcgcttt ggcggaatta
     3001 ctgggggcga agtggaaatg ctaaagatta ccaattcagt tattccaata gctggcaacg
     3061 catcagttat actttctctg ccagccaatc ctatgatgaa aacgacaaag aagaggagcg
     3121 ttttaatctg tttatctcca ttcctttcta ctggggggat gatattgcca aaacacgtca
     3181 ccaaattaac ttatcgaatt cgacctcatt ttccaaagat ggctattcct ccaacaatac
     3241 tggaattact ggcatagccg gtgaacatga tcagttaaat tatggtatat atgttaatca
     3301 gcaacaacaa aataatgata catcgcttgg tacgaattta agctggagaa ctcccatcgc
     3361 cacaatagat ggcagctata gccattctaa aaacgcctgg caaagtggtg gaagtattag
     3421 tagtggatta gttgtctggc ccggtggtat taatatcact aaccagctat ccgatacatt
     3481 tgcaattctg gatgcgcctg ggttagaagg cgcgcatatt aatggacaaa aatacaaccg
     3541 aacaaacagc aaaggccagg ttgtttacga cctgatgata cctcatcgtg aaaaccatct
     3601 ggtacttgat acagcaaaca gtgaaagtga aacagaattg cagggcaatc gtcaaattat
     3661 tgcgccttac cgtggagcag tttcttatgt gcagtttaca actgaccaac gtaagccctg
     3721 gtatatacag gcactgcgtc ccgatggttc gccattaact tttggctatg acgtactgga
     3781 tctccaggaa aacaatattg gagtcgttgg ccagggtagt cgccttttta ttcgcgtaga
     3841 tgaaattcca actggcataa aagttgctct caatgatgaa cagaatttat tctgtactat
     3901 tacttttcaa cacgttatcg atgaaaacaa aacatatata tgccagtaaa aggagtttac
     3961 tatgaagtgg cttttattaa tcacattgag tctatattct tttattgtgc aaagtgctcc
     4021 ctgtggattg accaatgtcg gggaacaacg tggaacatat atactcaaac cactgagtat
     4081 gaaaggtaat ctcaccgccg aaaaaattga aaatactggg aatatcattg agtgtgcaga
     4141 cttgcaagag acgaccaacg ccattaaagt tcatttttta acacagaacg ctttaaggtt
     4201 taaagtcgga agtcgtcatt atattattaa tttccccttt gaatctgacg gtaaaaaaaa
     4261 tagcgcttcc agggaagtca tcttataatt tagaagaaat gctatctgcg ataaatttca
     4321 ccattccata ctctatcgca tctgtatcag atagttccaa caccgcagat atttttccag
     4381 gaactccttt tcctttgttg ttcgaaattg aagtgagttc atgtacaggg aataatacta
     4441 attgtaatag cgtccgcttt aattttaatt taaatactat tttacaggta aatttaatga
     4501 cctgtggttt tgaagatcaa tccatcgaca ctggacgatt tgtactgtct cgtatcaata
     4561 atgaaaatta tcagtttcat catataagat ttgactgcat ccagggcgag caaggtgaat
     4621 tagtttttga acccagtgac gttgaatatt actttgaacc agtgacgata caggagtcag
     4681 gaaccagcat attaaaaaat gagttagaaa acagtgagga cggtgcgggt caaataggct
     4741 ttcaactctc caatgatggt acgaatgaaa tccagtatgg taaaagcaat tactactctt
     4801 ttcagcatcc ccatgaagga agtaatcaga ttcctctatt tattcgcccg cgtacatatg
     4861 gaaacaatgt ctcaagcggg caaataatga gccgagtcaa aatcgtggta atgtacaact
     4921 aatctatctc ccccacctta actttggggg agctgccgat taacgcaccg ctggactaaa
     4981 aagcgcagaa taatccgtat tgcagattcg ctgtaccgcc gggtgctgaa tcatccgctc
     5041 agcaaaaata gcatggtact cttccatcac attctcgacg cgaccaattt ctacgacagt
     5101 tttatcggca taaaagtcat atgcataaag cgttggggca acgaagattg cattgtgcat
     5161 cgcaccaaaa gctttcatca aagcggcatc atcaaactcg ccaaggattt ctacgtttaa
     5221 tccctgggag ttaaaccagt taagtaattt gcgccctaac attgaacgtc gcccaggaat
     5281 caaaagccgc cgttcttcaa gacaagctgg gaacggtttt tccggcggtg gatttgtaca
     5341 ccagaaactc acgccacatt cgccaattct cacggagaac aggccttcct gctgcgtaga
     5401 atctatcgga cagtcggaaa tgatcatatc cagtttatgc tggcttaatt gctccagcag
     5461 catttcgtgg gtggattcga agcagcgaag atgaatcggc tcgccttcta ctactgcagc
     5521 attcagcacg ctactgacca gacgtttgga aagtacatca gccacgccaa cgtcaaacaa
     5581 taaattggat tctttgcgat agttcacgat atccagcatt tcctggctca aggtaaacat
     5641 tttatcggca tagcgataga ccagctcccc cagctcgctg ggctcgagac cacgtccctt
     5701 gcgcttaaat aatttgcctt gcaggcgctc ttccagcgcc cgaatctgtc cggtaatggt
     5761 ttgtggtgtt aaataaagcg cctccgctgc gccaaccaca gaaccttctt tataaacatg
     5821 ccagaagtaa tacaagtggt tgtaattgat atgagacatg ctcatttctc tccctgataa
     5881 caatgaaaag ggagccgttt atggctcccc ggtaaaccgt cctgtcaaac tgatggacgc
     5941 aaacgaacgc gtaaccagct gtatccaatt accgccgaag agatagaacc gaccaggata
     6001 ccgagtttcg cccagttaat cagttctgga tctacgctac caaaggccag gctggcaata
     6061 aagatagaca tagtaaaacc gataccgcac aggatcccca ccgccataat ttgctgataa
     6121 gtcgttccct caggcagatg cgccagtttc aaacgcagcg ccaaccagca gaacagacta
     6181 atccccagcg gtttgccaat cagcaagcca gcgatgatcc ccaatggcag aatggaggtc
     6241 aagccatcca gcgtgacgcc ttgcagtgaa acgccagcat tagcaaatgc aaacagtggt
     6301 aaaatcagat acgccaccca cgggtgcaac acatgctcca gtcgcttcgc tggagaacgc
     6361 ccatgcttct ctttcaaagg aataaagaag ccgacaatta cccccgccag ggttgcgtga
     6421 acccccgatt tcaacactgc agtccacaac actacgccaa ccagaatata gacgcccgtg
     6481 cggcgtacac cacacagatt caataccgcg agtacagcaa ttgctacagc cgcgacgcca
     6541 agagaggcca tcgataagtc attagtgtag aacaatgcga tgataatgat ggccccaaga
     6601 tcgtcgataa tagccagagc catcaaaaag atcttcagcg ctaacggaac acgacttccc
     6661 aatagcgcca gcacaccaag tgcaaaggca atgtcagtag ccgccgggat cgcccaccct
     6721 tcgcgggtaa tcggatcggc atagttaaag gccagataga gtaatgccgg cacaatcatc
     6781 ccaccaatag cagcgataac tggaaatgcg gcctggcgta agctggctag cgatccttgc
     6841 atcagttcac gtttaacttc cagaccgact aacaggaaaa ataccgccat cagcgcgtca
     6901 tttatccata acagcatgtt tttgttgatt tcgagtgaac caacccggag ctgaaccggc
     6961 gtctccagaa agtcgtgata ccatccactg gttgcgccgc tgttggccat catcatcgcc
     7021 aggatagcgg caatgataag aataatgcct cccgaggcat cactgctaaa gaatcgatgc
     7081 agatgtttca ctttttattt ctctttcagg tgaatagatc gtttttacta ttttaccccg
     7141 cacgaatcat cgttaaaatc agattatagt gtaggaataa ttcgtttttt acgatctata
     7201 gaagaatttg cgccatcagc ggtgagatgc gtttttgacg gctgtaagcg ctgacgggtg
     7261 accctaagcc ctgataatca tagcttaagg tgaggatttc tgagggatta attaggaaaa
     7321 gtaataatta atttgttgtc agtcggcgat gtttgttagg ccaatatttc aaaaataatc
     7381 cagcttgtcg tcaataattg cgatgaagag ataaattaaa gttaaataat taaatcatta
     7441 aaaaattacg ccataaaaat gcgaggcgtc ttccggatta gaatcgataa aacagaatgg
     7501 gttaaagaga gcgatataat agcggcgggt gcttgaggct gtctgtctca ggcattagct
     7561 gaacagcaga tagagaaaag ccccgagtga tattttacca tcaacccgag gccccctata
     7621 tgctgaacac atgtagagtg cctcttactg accgtaaggt caaggagaag agagcaatga
     7681 agcagcataa ggtgatgatt gtcgccctga tcgtcatctg tatcaccgcc gtagtggcgg
     7741 cgctggtaac gagaaaagac ctctgtgagg ttcacatccg aactggccag acggaggttg
     7801 ctgttttcac ggcttacgaa tccgagtaag agcaacggcg gggagtgatc cccgccactt
     7861 tacaggtgct cgcatatctt caacgcaccc tatttttacc caggcctgcc cacgggcagg
     7921 cttttgggga ggttagcggg tcaggtcgtc aaaaaacttc ttcacaccat caaagaagct
     7981 ctttgagcgc gggctgttgt gctcgccggt tgggccaccg aagctttctt gcagctcttg
     8041 cagcagctgt ttctgcttct cgttcaggcc taccggtgtt tcgacgacaa cgcggcacag
     8101 caaatcaccc tgtgcgccac cgcggacaga cttgacgcct ttaccgcgca tacggaatag
     8161 cttaccggtc tgggtttcgc caggcacttt cagtttgacg cgaccatcaa gggtcggtac
     8221 ttcgatttca ccacccagcg ccgccatagc gaagttgatc gggacttcgc aatacaggtt
     8281 gttgccttca cgctcgaaaa tcgggtgctg tttaacctga acctgaacgt acagatcgcc
     8341 tgccggtgcg ccgtgttcac ccgcttcacc ttcgcccgca agacggatgc ggtctccagt
     8401 gtccacccct gccgggattt taacggacag cgttttgctg cgctcaacac gaccatgacc
     8461 atgacatttg ttgcacggat ctttgatcag cgtaccgcgg ccctgacagt gtggacaggt
     8521 ctgctgcacg gcaaagaaac cctggcgcat ctgcacctga ccagaaccat gacaggtcgg
     8581 acaggtctgc ggctgtgtac ctggttttgc accgctaccg tggcaaacgt cacactcttc
     8641 cagagtcgga atgcggatct ctttggtcac gccacgtaca gcttcttcga gggtgagctc
     8701 catgttatag cgtaaatcag caccgcgcgc cgcacgttga cgaccacgtc cgccgccaaa
     8761 aatatcgccg aaaacgtcac caaaaatatc gctgaagtct gcgccgccgc caaaaccgcc
     8821 gccgcccatg ccaccttgct caaacgcagc atgaccatac tgatcgtatg ccgcacgttt
     8881 ttgcgagtcg gtcagaactt cataagcttc cttgatctct ttaaatttcg cctcggcctc
     8941 tttgtcaccc tggttacggt ccgggtggta tttcatggcc aggcgtttgt aggccttttt
     9001 gatttcacgc tcttccgctg ttttggaaac gcctaaaatc tcgtaataat cttgcttagc
     9061 catctttttt aaattgcccc tagatgaatg cacgggcgga gaggaaattc cccttcgccc
     9121 gtgtcagtat aattacccgt ttatagggcg attatttttt gtctttgact tcttcaaatt
     9181 cagcgtcgac aacatcgtca tctttcgcgt tgtttgcaga agcatcagca ccggcagtct
     9241 gctgctgggc atgttgctgc tgggcgattt ccatcagttt ctgggaaacc tgtgccagtt
     9301 cctgcatttt cgcttcgata gcggctttgt cttcaccttt cagagcagtt tccagtgcag
     9361 tcagcgcaga ctcgatagca gttttgtcgt cagccggcag tttgtcgcct gcttcttcaa
     9421 cctgcttacg ggtgctgtgc agcagatggt cgccctggtt gcgagtctgt accagctctt
     9481 caaacttacg gtcagcttcg gcgttagctt cggcgtcgcg taccattttc tggatttcat
     9541 cttcgttcag accagaagac gccttgatgg tgatcttctg ctctttaccg ctgtttttat
     9601 ctttcgcgga aacgtgcagg ataccgtcag catcgatatc gaaggtaact tcgatctgcg
     9661 gcatgccgcg cggtgccggg ttgataccat ccaggttgaa ctgacccaga gatttgttgt
     9721 cagccgcacg tttacgttca ccctgcagca catggatggt taccgcagac tggttgtctt
     9781 cagcggtaga gaacacctgg ctgtgcttgg tcgggatagt ggtgtttttc gcgatcagcg
     9841 tcgtcatcac accgcccatg gtttcgatac ccagagacag cggggtaaca tccagcagca
     9901 gtacgtcttt tacgtcacca gtcagaacac caccctgaac agcagcaccg attgctacag
     9961 cttcgtccgg gttaacgtct ttacgcggct ctttaccaaa gaactcagca actttcttct
    10021 gaaccattgg catacgagtc tgaccaccaa cgaggataac gtcgtcgata tcagatacgg
    10081 acaggccagc gtcctgcagc gcaactttca gcggctcgat ggaacggtta accagatctt
    10141 caaccaggct ttccagtttc gcacgagtca ctttgatgtt catgtgtttc ggaccggtcg
    10201 cgtctgcagt gatgtacggc aggttaacgt cggtctgctg agcggaagac agttcgattt
    10261 tcgctttttc tgccgcttct ttcaggcgct gcattgccag cggatcgttg cgcaggtcaa
    10321 tgccctgatc tttcttgaat tcttcaacca gatagttgat cagacggctg tcgaagtctt
    10381 caccacccag gtgggtatca ccgttggttg ccagaacttc gaaggttttt tcgccgtcaa
    10441 cttcgtcgat ttcgataata gaaatatcga aagtaccacc acccaagtca taaaccgcga
    10501 tagtacggtt gccagtacct ttgtccagac cgtaagccag cgcagctgcg gtcggttcgt
    10561 tgatgatacg ttttacttcc agaccagcga tacggcctgc gtctttggtt gcctgacgct
    10621 gagcatcgtt aaagtatgcc ggtacggtga taacagcttc agttaccggt tcacccaggt
    10681 aatcttcagc ggttttcttc atttttttca gcacttcagc agaaatctgc ggcggtgcca
    10741 ttttctggcc tttaacttcg acccatgcgt cgccgttatc agcagcaata attttgaacg
    10801 gcatgatgga aacatcacgc tgtacttctt cgtcctggaa gcggcggcca atcaggcgtt
    10861 taatcgcaaa cagggtgttt tgcgggttcg tcactgcctg acgtttagcc ggctgaccaa
    10921 ccagagtttc accatcctgg gtataggcaa tgatagaagg cgtggtgcga tcgccttcgg
    10981 cgttctccag cacacgagga gtggtgccat ccataatcgc tacacaagag ttggtagtac
    11041 ccaggtcgat accaattatt ttacccatct aaacgtctcc actatatatt cggtcatcat
    11101 gtggttgtga gtctgtaata ggggcgaaac gtctggtttc aactgcccaa tttcattttt
    11161 ttgcagactc actcactgcg gttgactact aaatggggtc gtaacccacg tcatcaaggg
    11221 ggagatgcaa aaattttttg tgcttttgcg cagaaatttc gcgatatggc aggacaagac
    11281 gtaaaattga cagcaatttt attcatataa agaatgaatc gcagtcaagg tattcatcaa
    11341 aaaattgtaa taaaaagaaa agattacgtg cctgaatctt ctctttatca gcagtaaact
    11401 agtgggtatt catcccccta cctcttccca ctaagagaat ccttatgaaa tccgttttta
    11461 cgatttccgc cagcctggcg attagcctga tgctgtgctg cacggcgcag gcaaacgacc
    11521 ataaactcct cggcgtcatt gcaatgccgc gtaacgaaac caacgctctg gcgctgaaac
    11581 ttcctgtttg tcgcattgtg aaacgcatac aactctctgc cgaccatggc gatttacagt
    11641 taagcggtgc atcggtttat ttcaaagccg cccgtagcgc cagtcagagc ctgaatattc
    11701 cttcagaaat aaaagaaggg caaaccactg actggatcaa cattaacagc gataacgaca
    11761 ataaacgctg cgtctcaaaa atcacctttt ccggtcatac ggtgaactca tcggatatgg
    11821 ccacgctgaa aattatcggc gacgactaac gccagaatat agaagccaca aaaaatgaat
    11881 gttaattacc tgaatgattc agatctggat tttctccagc attgtagtga ggaacagtta
    11941 gcaaatttcg cccggctgct cacccataat gaaaaaggca aaactcgcct ctccagcgta
    12001 ctgatgcgca acgaactgtt taaatcgatg gaaggacatc ccgagcaaca tcgccgcaac
    12061 tggcagctga ttgctggaga attacagcat tttgttggcg atagtatcgc caacaaactg
    12121 cgcggacacg gtaaattgta tcgggccatt ttgctcgatg tttcaaaacg attgaagctg
    12181 aaagccgaca aagagatgtc tacgtttgaa attgagcaac agttactgga acaatttctg
    12241 cgtaatacct ggaagaaaat ggacgaggaa cataagcagg agtttctgca cgcggtcgat
    12301 gccagggtga atgagctgga agagctgctg ccgctgctga tgaaagacaa attattggca
    12361 aaaggcgtgt cgcatctgct ttccagccaa ctgacccgca ttttacgcac ccacgcagca
    12421 atgagcgtac ttgggcatgg tttgctgcgc ggcgcggggc tgggaggccc tgtaggcgcg
    12481 gcactaaatg gggttaaagc ggtcagcggc agctcctatc gcgtgacgat tccagccgta
    12541 ctgcaaatcg cctgcctgcg ccagatggtt agcgccactc aggtctgata ccggaaaccg
    12601 gggaaaattt tcattttccc ctcaaaagat cgtagacact gccccactgg ctgattatta
    12661 tgccgcgccc tgaaaacact acagttattc agggaaatta tttcaccatt cattcgatga
    12721 tgatttttga ggaattatgg gcaacactaa gttggctaat ccggcaccgc tgggcctgat
    12781 gggcttcggc atgaccacca ttctgcttaa cctgcacaac gtaggttatt tcgctctgga
    12841 cggtattatt cttgccatgg gcattttcta cggcggcatc gcgcaaattt ttgctggtct
    12901 gctggagtac aaaaaaggca acactttcgg tttaaccgca ttcacctctt acggttcttt
    12961 ctggctgacg ctggttgcga ttctgctgat gccgaaactg ggtctgaccg atgcgccaaa
    13021 tgcacagttc cttggtgtct acctgggtct gtggggcgta tttacgctgt ttatgttctt
    13081 cggcacgctg aaaggcgcac gcgttctgca attcgttttc tttagcctga ccgtgctgtt
    13141 tgccctgctg gcgatcggta acattgccgg taacgccgca atcatccact ttgccggctg
    13201 gattggtctg atctgcggtg ccagcgcaat ctatctggcg atgggtgaag tactgaacga
    13261 gcagtttggt cgcaccgttc tgccgattgg tgaatcccac taattgtctt gagatccccc
    13321 cgctcggggg gattttttta ttcgctaacg tcgcgtcttg cactcttcgg tctgaatgca
    13381 gcaaccactt ctggtgccgt ttcaacgtat ggcccttcca gcaactgaat gcagtacggt
    13441 acgctggcaa aaataccgtg taccacaacg ttaccctcag cgtccttcac accttccagc
    13501 gtctctttaa tagacttcgg ctgaccgggt aagttaagga tcagcgcctg tttgcgaatc
    13561 acacccacct gacgcgaaag gatcgcagtt ggtacaaaat gcaggctgat ctggcgcatc
    13621 tgttcaccaa agccaggcat ctcgcggtcc gctactgcca gcgtcgcatc gggcgttacg
    13681 tcacgacgcg ccgggccagt tccgcccgtg gtgagcacca gatggcaact catttcatcc
    13741 accagctcac acaacgtttg ctcgatgatc gcctgctcat cggggattaa gcgggtttcc
    13801 agttcaaacg gcgtggttag cgccgatgtc agccattctt ccagcgcagg gataccttta
    13861 tcctgataaa cgccgctgga tgcgcgatca gagatggaaa ctaagccaat acgtaaagta
    13921 ttcatcaaga tttttccgtt taaacagtct cgttaaacag aatgatacac tgcgaaggga
    13981 gtgacagaca ggcttcagaa gaggtagcgt gaccgacttc ccggtcacgc taagaatgat
    14041 tacagcagat cgccgatcat tttttccagt ttttcctggt caatagcaaa cttacggata
    14101 ccttccgcca gtttatctac tgccattgga tcctggttgt gctgccacag gaactcggac
    14161 tcagtgatac gcgccggacg cgctttcact tcgccggtgt aagacagttt acgttcgata
    14221 gccccttcgc tctcagccag ctctttcagc agtgccggtg cgatggtcag acggtcgcag
    14281 cctgccagtt ccagaatttc gccgatgtta cggaagcttg cgcccataac cacggtttca
    14341 taaccgtgct ctttgtagta ctggtagatt tcagatacag aaaccacgcc cggatcttct
    14401 gccggagcgt actctttctt atcggtattc gctttgtacc agtcaagaat acggccaaca
    14461 aacggcgaga tcaggaacac gcccgcttcc gcacaagcac gagcctgagc gaaggagaac
    14521 agcagggtca ggttacagtt gataccttct ttttccagct gttccgcagc acggataccc
    14581 tgccaggtag aagccagttt gatcagaata cgatcgttgc taatacctgc atcgttgtag
    14641 agtttgatca ggcgttttgc tttcgcaatt gacgcttctg tgtcatagga aagacgcgca
    14701 tcaacttcag ttgagatacg gcccggaacc agtttcagga tttccagacc aatatttact
    14761 gccagtttgt cagtcgcgtc cacgatctgc tgcgcgcgat cgttgctctg ctgtttcgcc
    14821 caggcgacag catcatcaat caacttacgg tattccggaa tctgcgctgc gttaagaatg
    14881 agagaagggt tggttgtggc atcctgcggt tgatacagct tcattgccgc gatgtcccca
    14941 gtgtcggcca ctacggtggt gtactgacga agggaggtca atttgtccgt catgatagta
    15001 tttctcttta aacagcttgt tagggggatg taaccggtct gccctgatga tatcacgacg
    15061 caacgtcagc gcaaccgccg acaagacggt tatcgccagg cgagactgtt tcggatttct
    15121 gacactgcgt tgatatcgcc cgccattttt aaacaaaacc tcatgtatgc tacgcagaag
    15181 ttatcaagta cctcgtagcg tatatacttc ttaaacaatt ggtaacgttt acacaggaaa
    15241 gtcatcgcga ccggcaataa gagggatatg catgccagat tttttctcct ttattaacag
    15301 cgtcctttgg ggatcggtaa tgatttacct gctcttcggc gcaggttgtt ggttcacttt
    15361 tcgcaccgga tttgtgcagt ttcgctacat ccgccagttt ggcaaaagtc ttaaaaatag
    15421 cattcatcca cagccaggcg gtttaacctc atttcagtca ttgtgtacca gtcttgcggc
    15481 gcgcgtgggt agcggcaacc tggccggcgt tgcgctggct attaccgccg gtggacctgg
    15541 agccgtcttt tggatgtggg ttgccgcgtt tatcggcatg gcgacctcgt ttgccgaatg
    15601 ttcccttgca caactttata aagaacgtga cgtcaatggg cagtttcgtg gcggaccggc
    15661 atggtatatg gcgcgcgggc tggggatgcg ctggatgggc gttctgttcg ccgtcttttt
    15721 gctcatcgcc tacggcataa ttttcagcgg agttcaggca aacgctgttg cccgagctct
    15781 gagtttttct tttgattttc ccccgctggt gacaggcatt attcttgctg tctttgctct
    15841 gctggcaatc attcgcggtc ttcatggcgt cgcccggctc atgcaggggt ttgtcccgtt
    15901 gatggcgata atctgggtac tgaccagcct ggtgatttgc gtaataaata tcgggcaact
    15961 tccccacgtc atttggtcta tttttgagag tgcttttggc tggcaggaag cggcaggcgg
    16021 cgcggcggga tataccttaa gccaggcgat tactaacggt tttcagcgca gtatgttttc
    16081 caatgaggcg ggaatggggt cgacgccaaa cgcggcagcg gcagcggcgt cctggcctcc
    16141 gcatccggca gcgcaaggaa ttgtccagat gattggcatt tttatcgaca ccctggtcat
    16201 ctgtacggca agcgccatgc tgatattact ggcgggtaac ggcacaacct acatgccgct
    16261 ggaaggtatt cagcttatcc agaaggcgat gcgggtgcta atgggttcct ggggtgctga
    16321 atttgttacc ctcgtggtta ttctgtttgc cttcagctcc atcgttgcca actacattta
    16381 tgccgaaaac aatctcttct ttttacgcct gaacaaccct aaagcgatct ggtgtttgcg
    16441 gatctgcacc ttcgcaacgg tcatcggcgg caccttgcta agtcttccgc tgatgtggca
    16501 actggcagat atcataatgg cctgcatggc tattaccaat ttgactgcca tattactgct
    16561 ctcgcctgtg gttcatacca ttgccagtga ttatctacgc cagcgtaaac tcggcgtgcg
    16621 cccggtgttt gatccgttgc gttatccgga tatcggtcgc cagctttctc gggacgcgtg
    16681 ggatgatgtt tcgcaggagt aatcacaact atcgatcaac tcattctctt tttttgctaa
    16741 agtcggcata aatttcctgc aaggactgga tatgctgatt cttatttcac ctgcgaaaac
    16801 gcttgattac caaagcccgt tgaccaccac gcgatataca ctgccggagc tgttagacaa
    16861 ttcccagcag ttgatccatg aagcgcggaa actgacgcct ccgcagatta gcacgctgat
    16921 gcgtatcagc gacaaactgg caggtatcaa cgccgctcgc tttcatgact ggcagccaga
    16981 tttcacgccg gagaatgccc gccaggcgat tctggcgttt aaaggtgatg tctacaccgg
    17041 cttgcaggcc gaaaccttca gcgaagacga tttcgatttt gcccaacagc atttgcgaat
    17101 gctttccggc ttgtatggcg tactccgccc gctcgattta atgcagcctt atcgtctgga
    17161 aatggggatc cgtcttgaga atgcccgagg gaaagatctg tatcaattct ggggagatat
    17221 catcaccaac aagctgaacg aggcgctcgc agcacaaggc gataatgtgg tgattaatct
    17281 ggcgtcagat gagtatttta aatcggtgaa gccgaagaaa ttgaatgccg agattatcaa
    17341 gccggtgttc ctcgatgaga agaacggcaa atttaagata atcagcttct acgctaagaa
    17401 agcacgcggt ctgatgagtc gtttcattat tgaaaatcgg ctgaccaaac cagagcaact
    17461 gactggtttt aatagcgaag gttacttctt tgatgaagct tcctccagca atggcgaact
    17521 ggtgtttaaa cgctacgagc agcgttaagt ttgtagtcaa tgccggatgc ggcgtgaacg
    17581 ccttatccgg cctacaaaag catgcagatt caatatattg cagaattcac gtaggcctga
    17641 taagacgcgc cagcgtcgca tcaggcgctg aatgccggat tgttacccgg catttgtcat
    17701 ttagcggtga tgtttgcctg gaccatgacc gccgtgatga tcatgatgag ctttcttatg
    17761 gtgacgcggc ggtggcggcg gtccgtgtgg gtgccagcga ttgcctcgcc attcataatg
    17821 ttgtttccac cagccgtggt cgcgccagtg accgccatcc cagtaataac cgcgattatc
    17881 acgatcgcct atctgtaatt ttactgacgg gactaacgta atttccgcag cctgcgttgc
    17941 catgggagcg accagaacca gggaaagtgc gagtacgata gattgcatct ttttcacagg
    18001 ttactccttt tatcattgcc cgttatgggt cgatatccgg agattacgcg atgagagcgg
    18061 cggttgttat tctccgcaat cctaatctct aagttaccgc atttattgag aatttctcct
    18121 ttttccctgt cgtttctctc catattttct tcacaaaaaa gccgggctgc ataaaagcaa
    18181 acccggcctg attgagataa tgaatagatg ttactgatga ttcatcatca atttacgcaa
    18241 cgcagcaaaa tcggcgggca gattatgcga aagcaagggt aaatcggcac gttctgccag
    18301 ctcttttggc agatccaacg tttcaccgag aatcgcttcc acgctctctt taaatttcgc
    18361 cggatgcgcg gtgccgagga acaagccata ttcgcctgga ttcaactggt cacgcagcgc
    18421 acgatacgct acggcagcgt gcggctccga agtgtagccc agttctttta actcacgcat
    18481 tgtctgttgc gtggtttcat catccacggc ggcataaccc agctctttca gttgccagat
    18541 tttgcggcgg aacaactctt ccacacgcgg ccagttgttc ggctgactca cgtccatcgc
    18601 gttggataac gtcgcctgag tcgctttggg tgaccactga ccgtcatgca ggaaacgtgg
    18661 cacggtatcg ttcacgttgg tcgcagcaat aaaacgtttc accggcagac cgagtgactt
    18721 cgccagcaga cccgccgtca aatcgccgaa gtttccgctt ggcaccgaga caaccagctg
    18781 gttgcgcgct tcctgcggca gctgcgcaac tgcttcaaag tagtagcaaa tctgcgccag
    18841 caaccggcta atgttaatcg agttagctga gtttaacccc agcgccactt tcagctcttc
    18901 atcatcaaac gcctgcttca ccagcgcctg acaggcatcg aaatcgccgt cgatggcaac
    18961 agtttcgata ttgccgccca atgtacagaa cagtttttct tgcagtggac tgattttgcc
    19021 tcgtggataa aggataacca ctttcacatt cggtaaaccg tagaaagcat gagccactgc
    19081 cgctccggta tcaccggagg tcgcggtcag aatggtcact ggcttatcgc ccgcaatatg
    19141 ggtcagcatt tgtgccataa agcgaccgcc gaaatcttta aatgccagcg ttggcccgtg
    19201 gaacaattcc agacaaccga catcgctttc aacattggcg accggagccg ggaaggcaaa
    19261 cgccgcgcgc acgcgctctt ccaggatttc ctgcgggatt tcatcaccaa taaacgccga
    19321 gaggatcttc gcactgcggg tgacaaaatc cagcttcagc atctcatcaa tttcagtcag
    19381 gctgaattcc ggcaggtcgt gcggaaaaaa cagcccctga tttttgccca acccctgggt
    19441 tacggcttgc gcaaagctga cctgctcatt gtgatcttta agattgtaga gtttcattta
    19501 gttttccagt actcgtgcgc ccgccgtatc cagccggcaa atatgaacaa aaccttcctg
    19561 attttgcagg tagttcttac ccaaccagtc ggcaacgcgc tgggcggtat ccggcttgtc
    19621 acacagagca aacaaggtcg ggccggagcc ggagataccg ctcgctaccg cgccgatttc
    19681 cgcgaccgcc tgccgcgcct gccggaagcc aggcagtaac cgttcacggt agggttctgc
    19741 gataacatct ttcatcagct tcgcggcaag ctcaggctga cgggaatagc aggcgtgaat
    19801 gaagcctgcc aagtgtcgcc cgtgcgcaat gcaatcctgg cggcgatact gcgccggtaa
    19861 aatagcccgg gcttctgccg tcgagacttt aatccccgga tacgccagca cccacagcca
    19921 ctcatcaaac cctggcactt gctggctgat gatgtcgttt tcttcgatca tcaactgcat
    19981 accaccaaga aaacacggtg ccacgttgtc gtaatgaatg ctgccggaga ttcgtccttc
    20041 cagctcgccc atcaaagcca gcaaacgggt gtcattaagc ggcttgccgc agtgttcatt
    20101 catcgccatc agagccgcga ccaccgaaca ggcgctggag cctaagcccg aaccgatcgg
    20161 catattcttt tccagagtca tcgccactgg aatttgcttg cccagctcct ggcaaaaacg
    20221 ctcccagcac tgataaacga tattttcccg tggttctgac ggcagcttat cggcaaagcg
    20281 tccgaggttg ttgagactga atgtctctgc cgactcaacc gtgactacat ctccgagcaa
    20341 tgcaccatca acgggtgtca ccgccgcccc gagcacatca aacccgacgc tcatattggc
    20401 actggaagcc ggggcataaa ctttaaccat gtcagactcc taacttccat gagagggtac
    20461 gtagcagatc ggcaaagaca ccggcagcgg taacgtcatt gcccgcacca tatccgcgca
    20521 gcaccaacgg cagcggctga taatagtggc tataaaaggc cagggcgttt tcgccatttt
    20581 tcactttgaa cagcggatca ttaccatcca cttcggcaat cttcacgcgg cagacgccat
    20641 cttcatcaat attgccaaca tagcgcaaaa cttttccttc atcacgggcc ttcgccacgc
    20701 gcgcggcaaa gagatcgtcg agctgtgaca gattcgccat aaaagcggca acatcaccct
    20761 cagcgttaaa ctctgcgggc agcacaggtt caatttcaat atccgccagc tccagttcac
    20821 gtcccgtttc acgagcgaga attaatagtt tacgcgctac atccatacca gaaagatcat
    20881 ctcgcggatc cggttcggta taacccattt cccgcgccag cgtagtcgcc tcggagaaac
    20941 tcatgccttc gtctaacttg ccgaagatat aagaaagcga acctgaaaga atgccggaga
    21001 acttcatcaa ttcatcacca gcattgagca gattttgcag gttctcaata accggtaatc
    21061 cagccccaac gttggtgtca tagaggaatt tacgccgcga tttttcagcc gcatgacgca
    21121 acagatggta gtaatccatc gacgaggtgt tggccttttt gttcggcgtg acaacgtgga
    21181 aaccttcgcg caggaagtcg gcatattgat ccgccactgc ctggctggag gtgcagtcaa
    21241 caatgaccgg gttcagcaga tgatattctt tcacgaggcg aattaagcgc ccgagattaa
    21301 acggctcttt ggcttgcgcc agttcttcct gccagttttc cagatttagg ccatgcacat
    21361 tggtgagcag agccttcgag ttagcaacac cgcagacacg taagtcgata tgtttattct
    21421 tcaaccagct ttgctgacgc ttcagttgct ccagcagcgc accgccaacg ccaccgacgc
    21481 caatcacaaa cacttcgata acctgatcgg tattgaacag catctgatga gtaacgcgca
    21541 cgccagtggt cgcatcatcg ttatttacca cgacagagat tgagcgttca gaagatccct
    21601 gagcaatagc gacaatgttg atattggcgc gggccagcgc ggcaaagaat ttcgccgaga
    21661 tcccacgcaa ggtgcgcata ccatcaccta ccaccgagat aatggccagc cgttccgtca
    21721 ccgccagcgg ctccagtaag ccttctttca gttccaggta gaactcttcc tgcattgccc
    21781 gttcagctcg cacacagtcg ctttgcggaa cgcagaaact gatactgtat tcggaagatg
    21841 attgcgtaat cagcaccacg gaaatacggg cgcgtgacat tgcagcaaag acgcgcgccg
    21901 ccatgccgac cattcctttc atccccgggc cggaaacgct gaacattgcc atattattca
    21961 gattggaaat gcccttgacc ggtaattcgt cttcatcacg actggcacca atgagcgtac
    22021 ctggagcttg aggatttccg gtatttttaa tcaggcaagg gatctggaac tgggcgatgg
    22081 gggtaatggt gcgggggtga agaactttag cgccgaagta ggaaagctcc atcgcttcct
    22141 ggtaggacat cgatttcaac aacctcgcat cgggcacctg acgcgggtcg caggtatata
    22201 ccccgtcaac gtccgtccaa atctcgcaac aatcggcgcg taaacaggca gccagcaccg
    22261 ccgcggagta gtcggaaccg ttgcgtccaa gtaccaccag ttcgcctttt tcattaccgg
    22321 cggtgaaacc tgccatcagc accatgtgat cagccggaat acgacttgcc gcaatacggc
    22381 gggtggactc tgcaatatcg acagtagatt cgaggtaatg ccccactgcc agcagttttt
    22441 cgaccggatc gataacggta acgttgtgac cgcgcgcttc taatacgccg gccataatgg
    22501 cgatcgacat tttctcgcca cggcaaatca gcgcagcgtt aatgctatcc gggcactgcc
    22561 ctaacaaact aatgccatgc aggacatgtt ttatttgggc aaattcctgg tcgacgaaag
    22621 ttttcaattg cgccagcggg aatcccggct gggcggcggc gagtcccgtc agaagttcgg
    22681 caaaaatacg ttcggcatcg ctgatattgg gtaaagcatc ctggccgcta atggtttttt
    22741 caatcatcgc caccaggtgg ttggtgattt tggcgggggc agagaggacg gtggccacct
    22801 gcccctgcct ggcattgctt tccagaatat cggcaacccg cagaaaacgt tctgcatttg
    22861 ccactgatgt accgccgaac ttcaacactc gcatggttgt tacctcgtta cctttggtcg
    22921 aaaaaaaagc ccgcactgtc aggtgcgggc ttttttctgt gtttcctgta cgcgtcagcc
    22981 cgcaccgtta cctgtggtaa tggtaatggt gatggtggtg gtgatggtgg tggtaatggt
    23041 ggtgctaatg cgtttcatgg atgttgtgta ctctgtaatt tttatctgtc tgtgcgctat
    23101 gcctatattg gttaaagtat ttagtgacct aagtcaataa aattttaatt tactcacggc
    23161 aggtaaccag ttcagaagct gctgtcagag actctttttt taatccacac agagacatat
    23221 tgcccgttgc agtcagaatg aaaagctgaa aaatacttac taaggcgttt tttatttggt
    23281 gatatttttt tcaatatcat gcagcaaacg gtgcaacatt gccgtgtctc gttgctctaa
    23341 aagccccagg cgttgttgta accagtcgac cagttttatg tcatctgcca ctgccagagt
    23401 cgtcagcaat gccatggctc gttcgcgtaa agcttgcagt tgatgttggt ctgccgttgt
    23461 gtcacttttc gtcggttgtt gtattaatat tgctaattga tagcaataga ccatcaccgc
    23521 ctgccccaga ttgagcgaag gataatccgc caccatcggc acaccagtaa gaacgtcagc
    23581 caacgctaac tcttcgttag tcagcccgga atcttcgcga ccaaacacca gcgcggcatg
    23641 gctcatccat gaagattttt cctctaacag cggcaccagt tctactggcg tagcgtagta
    23701 atgatatttc gcccgactgc gcgcagtggt ggcgacagtg aaatcgacat cgtgtaacga
    23761 ttcagccagt gtcgggaaaa ctttaatatt atcaataata tcaccagatc catgtgcgac
    23821 ccagcgggtg gctggctcca ggtgtgcctg actatcgaca atccgcagtt cgctaaaccc
    23881 catcgttttc attgcccgcg ccgctgcccc aatattttct gctctggcgg gtgcgaccag
    23941 aataatcgtt atacgcatat tgccactctt cttgaccaaa tgaccgcgaa cagggtgatc
    24001 actgtcaact tattacgcgg tgcgaattta caaattctta acgtaagtcg cagaaaaagc
    24061 cctttactta gcttaaaaaa ggctaaacta tttcctgact gtactaacgg tttaattgtt
    24121 aaaaaatgct acatatcctt ctgtttactt aggataattt tataaaaaat aaatctcgac
    24181 aattggattc accacgttta ttagttgtat gatgcaacta gttggattat taaaataatg
    24241 tgacgaaagc tagcatttag atacgatgat ttcatcaaac tgttaacgtg ctacaattga
    24301 acttgatata tgtcaacgaa gcgtagtttt attgggtgtc cggcccctct tagcctgtta
    24361 tgttgctgtt aaaatggtta ggatgacagc cgtttttgac actgtcgggt cctgagggaa
    24421 agtacccacg accaagctaa tgatgttgtt gacgttgatg gaaagtgcat caagaacgca
    24481 attacgtact ttagtcatgt tacgccgatc atgttaattt gcagcatgca tcaggcaggt
    24541 cagggacttt tgtacttcct gtttcgattt agttggcaat ttaggtagca aacatgcaga
    24601 ccccgcacat tcttatcgtt gaagacgagt tggtaacacg caacacgttg aaaagtattt
    24661 tcgaagcgga aggctatgat gttttcgaag cgacagatgg cgcggaaatg catcagatcc
    24721 tctctgaata tgacatcaac ctggtgatca tggatatcaa tctgccgggt aagaacggtc
    24781 ttctgttagc gcgtgaactg cgcgagcagg cgaatgttgc gttgatgttc ctgactggcc
    24841 gtgacaacga agtcgataaa attctcggcc tcgaaatcgg tgcagatgac tacatcacca
    24901 aaccgttcaa cccgcgtgaa ctgacgattc gtgcacgcaa cctgctgtcc cgtaccatga
    24961 atctgggtac tgtcagcgaa gaacgtcgta gcgttgaaag ctacaagttc aatggttggg
    25021 aactggacat caacagccgt tctttgatcg gccctgatgg cgagcagtac aagctgccgc
    25081 gcagtgagtt ccgcgccatg cttcacttct gtgaaaaccc aggcaaaatt cagtcccgtg
    25141 ctgaactgct gaagaaaatg accggccgtg agctgaaacc gcacgaccgt actgtagacg
    25201 tgacgatccg ccgtattcgt aaacatttcg aatctacgcc ggatacgccg gaaatcatcg
    25261 ccaccattca cggtgaaggt tatcgcttct gcggtgatct ggaagattaa tcggctttac
    25321 caccgtcaaa aaaaacggcg ctttttagcg ccgtttttat ttttcaacct tatttccaga
    25381 tacgtaattc atcgtccgtt gtaacttctt tactggcttt cattttcggc agtgaaaacg
    25441 cataccagtc gatattacga gtcacaaaca tcatgccggc cagcgccacc accagcacac
    25501 tggttcccaa caacagcgcg ctatcggcag agttgaggag tccccacatc acaccatcca
    25561 gcaacaacag cgcgagggta aacaacatgc tgttgcgcca acctttcaat accgcctgca
    25621 aataaatacc gttcattaac gccccaatca gactggcgat tatccatgcc acggtaaaac
    25681 cggtatgttc agaaagcgcc agcaagagca aataaaacat caccaatgaa agccccacca
    25741 gcaaatattg cattgggtgt aaacgttgcg cggtgagcgt ttcaaaaaca aagaacgcca
    25801 taaaagtcag cgcaatcagc agaatggcat acttagtcgc ccggtcagtt aattggtatt
    25861 gatcggcggg cgtcgtcact gcgacgctaa acgcggggaa gttttcccag ccggtatcat
    25921 tgcctgaagc aaaacgctca ccgagattat tagcaaacca gctgctttgc cattgcgcct
    25981 gaaaacctga ctcactaact tcccgtttgg ctggtagaaa atcacctaaa aaactgggat
    26041 gcggccagtt gctggttaag gtcatttcgc tattacgccc cgcaggcacc acagaaagat
    26101 cgccggtacc gcttaaattc agtgccatat tctgcttcag gttctgcttc cgccagtccc
    26161 cttcgggtaa agggatatgc acgccctgcc cgccttgctc taacccgatg ccgggttcaa
    26221 tagtcagcgc cgttccgtta acttcaggcg ctttcaccac accaatacca cgcgcatccc
    26281 cgacgctaat cacaataaat ggcttgccaa aggtgatatt tggcgcgtcg agttcgctaa
    26341 gacgcgaaac atcgaaatcg gcttttagcg ttaaatcact gtgccagacc tgaccggtat
    26401 aaatccctat cttgcgttct tccacgttct gattgccatc aaccatcaat gactcaggta
    26461 accaaaaatg gataaaactt cgtttccgct ccacggtttt atcctcttcc tgcaccgtat
    26521 aaagctcggt cacaggaata gcgataagag gcccaacgag tttttgcggc ccgctggtac
    26581 tttggcgaat cgcatcttcc acatcgctac ggtaatcggc acgttcgaca atcacctgcc
    26641 gaatcagcat tatcggaatc aacaacagca atactgcacc aaacagggtg gtcattttcc
    26701 agaacagggg ggatttcaac atagtcttct cctttgcaat ggcagcagga tacgaaggct
    26761 atgtgggaag aatttgaagc tatgtgaagt gacggtgaag ccgaagcgag gccagcacgc
    26821 caccttcctg cacgttgcgc agcgtgactt cgccgttaaa caaacgggcg acctcactga
    26881 caaacgccaa ccccagaccg ctgctttttt gcccatttgc acgcggcaaa gagtaaaagc
    26941 gttcaaaaat acgtgacagc gcgtagtcag gaataccact accggtatcc agcaccttaa
    27001 gggcgacgtg ttcctgatcc acttcggcgc ttagcgttat gcgaccgctc ttgggggtaa
    27061 aatcgatggc gttatccagt aaattcccca gcgcctgctc cagtaacgcc ggttcagcag
    27121 caacgttaac ctcagtaggc ataacatgca aagtgatgtt tttttctgcc aactgcacgg
    27181 tgcgcgcttc gctgacgcgg cgaaataatg ccgccacatc aacagcagtc agaacgactt
    27241 cctgacgatt ctccagtctt gcctggcgta gtaacgtttc taccaatgcc tgcatacgcg
    27301 cattttgcgt cagaatgttg tcggtaaaac gagccaccac ttccggtggc ggaccttcgc
    27361 gtaaaatttc cgccgcgcca cgaatcgccg ccagtgggct ttttagctca tgggttaacg
    27421 cataaacata ctgctcaata tagtttttcc cttccagctt cacgcgcata ctttccagcg
    27481 cctgcgcaag cttacgcaac tcgctactgc cgagttcagg aagaggaacg ggcttattgt
    27541 cagtgacgga atcggcatag cgagtgagcc gggcaatgga gcggttgatc caccaaacca
    27601 tgcctgcgcc aatcaccagt gcaatcccca gcaaaatggc gctggcccat aaaattcgcc
    27661 gctcgctgcg cttaatgacc ggagccatcg ccgcgttcgg tttgcctacg ctcaaaacgc
    27721 caataagccg cgagccgtcc ataatcggcg cggcaacata catcaccgaa ctttcgggat
    27781 cggcaggatt ttgcaacgtg ctgcgcgcac catactgacc acgcaacgtt agccagacgt
    27841 cattccagcg cgaataatcc tgcccaacgg ctttatttgc cgaatcgaac aatactttgc
    27901 cctgcgcatc ggtcatatag acatgatatt cattgcgcac tttgttaatg ccaccgatat
    27961 tagcgcgaaa cgggcgatgt tgtagctgat taaacgcctg cgccagttgc ccatgcgttg
    28021 ggtccccaga gagcaaatcg ggacgcgcca gctccgccag caacgttgcg gtgtcgatca
    28081 gcgtcccctc cgttgctctt cgcacgcccg gtttaacttc tttgacaaaa atagccagta
    28141 cgaaccaggc cgccaccgcc accagtaaaa aatagcccag caacaaccgc atgccgatac
    28201 gcattacagg ctcctcaggc tgtatcccat gccgcgatga gtattaatcg gtgaaagatc
    28261 ggggttgatg gcgcgcagct tggcacgcag cgttttaatg tgggtatcga cggtgcgatc
    28321 gtaggtgtcc tgcgcatctt cccacacgat atccatcagt tgctggcggg accagacgcg
    28381 gcctggcgac ttgagtaacg tcttcagcaa taaaaactca taccgcgtca gcgttaatgg
    28441 cgtgtcaaac cagctgatct gcgccgcggg ttcattcaat tcaaaatgac caatacggat
    28501 gacgggagac ggcgtcgaga acttcttcac ccgacgcagc aaggtgcgca ccctagcgca
    28561 cacttcgcgg ggtgaaaacg gtttagccac atagtcgtca gcgccaattt ccagcccaag
    28621 caggcgatcg acctcttcac ttcgggccgt caggaacagt acaggtaacg ccggatggag
    28681 cgccagtaac tggcggcaca attcaaagcc actgatatca ggcaaaccaa cgtcgagaat
    28741 catgacatcg ggaacctgct ggcgagcttt atccagcacc ggcaagcctc gctcaaagac
    28801 ctcgacggca aaaccttcct gctgcaacat gtagaccagc gtgtcggcta tcccttgctc
    28861 atcttccact aaccagaccg tttcccgttg cataaatcgc ctctgttatt gtcgccacgg
    28921 catgacagga accgcgctaa tcgcgttttt cggcgaacct tctacaactt tgtcggagta
    28981 agccagataa gcgagcgcgt tgcgtttggc gtcataaaag cgcacgacct gtaacgactt
    29041 aaagaccagg gacgtgcgtt ttttgaatac tacctcgccc tgagctttgc cgtttttaat
    29101 acgatccgac agttcaatcg gcccgacttg ctgacaagaa atagccgcat cggaggtatc
    29161 ttccgccaga cccaatcccc ctttaatacc acctgttttc gcccggctca cataacaggt
    29221 gacatttttc acatcgggat cgtcaaaggc ttccacaaca attttgtgat ccgggccgat
    29281 cattttaaat acggtgtcga ccgaaccaat ctcttctgca tgagccaatg gccccagcat
    29341 tatcaggctt aaagacagga tcaaatgctt gtatttcata ttgttaccat ttttatctat
    29401 tacacgagca attagttcgt cacggtaaat aacatattga ttaagaacaa aatctcaata
    29461 cttttatttc cgtcaagacc taaaacatac tctactaaga aaaaaacact gaatgctaaa
    29521 acagcaaaaa atgctattat ccaattacct gatgtcaggt gctcgttgtt gaaaggatga
    29581 ggatatttta tggatcaggc cggcattatt cgcgaccttt taatctggct ggaaggtcat
    29641 ctggatcagc ccctgtcgct cgacaatgta gcggcgaaag caggttattc caagtggcac
    29701 ttacagagaa tgtttaaaga tgtcactggc catgctattg gcgcgtatat tcgtgctcgt
    29761 cgtttgtcga aatcggcggt cgcactacgc ctgactgcgc gtccgattct ggacatcgcg
    29821 ctgcaatacc gcttcgactc tcaacagaca tttacccgcg cattcaagaa gcagtttgcc
    29881 cagactcctg cactttaccg ccgttctcct gaatggagcg cctttggtat tcgcccgccg
    29941 ctgcgtctgg gtgaattcac tatgccagag cataaatttg tcaccctgga agatacgccg
    30001 ctgattggtg ttacccagag ctactcctgt tcgctggagc aaatctctga tttccgccat
    30061 gaaatgcgtt atcagttctg gcacgatttt ctcggcaacg cgccgaccat tccgccagtg
    30121 ctctacggcc tgaatgaaac gcgtccgagt caggataaag acgacgaaca agaggtattc
    30181 tataccaccg cgttagccca ggatcaggca gatggctatg tactgacggg gcatccggtg
    30241 atgctgcagg gcggcgaata tgtgatgttt acctatgaag gtctgggaac cggcgtgcag
    30301 gagtttatcc tgacggtata cggaacgtgc atgccaatgc tcaacctgac gcgccgtaaa
    30361 ggtcaggata ttgagcgata ctacccggca gaagacgcca aagcgggaga tcgcccaatt
    30421 aatctgcgct gtgaactgct gattcctatc cgccgttaac gctgcagctc atctaatgca
    30481 ggggcgtcca gatgcgagat gtcccctgcg gtttcaacga cccagccaga cgccagccac
    30541 aggctttcct gataatccac gcgcgaaata gaacagttac gcagacgtaa gcggcgctct
    30601 gcccatgctg gtaatccgag aatcgtactc accagacatc ccagtgcaat accatggctc
    30661 accagcagcg gtcggcttcc ctgcggtaag tcccggcaag attccagtgc cgcattgacg
    30721 cgatcgctga gctcctgcat tgactcgcct tcaggaatac gcccatcaac ggtgccattg
    30781 accagctgcc gacgccagtt ctcttcttct tcggtcagag aatcgatatg tcttttttcc
    30841 agcacaccca tgtttaattc acgcaggcga gaatcaaaga tgatgtcaca gccgcaggcc
    30901 tgggcgatga tttccgccgt acgccgggtg cgtcctaaat cgctactgat gatatgcgta
    30961 atgccaagct ctttggcacg cgtcgccacc tgcatcgcct gttgctcacc tttggcggtc
    31021 agcgggctgt cagactggcc ctgaatacgt cgctcggcgt tccactgcgt ttcaccgtgg
    31081 cggactaggt atacctgtaa catgcttttt ttccgtatac tgctgtaacg ttaattttct
    31141 gagtaatgct gattatgcac caagttgtct gtgcgaccac caatcccgct aaaattcagg
    31201 ccattctgca ggcatttcac gagatcttcg gcgaaggatc ctgccatatt gcatccattg
    31261 ccgtcgagag cggtgtaccg gaacagccct ttggcagtga ggaaacgcgc gctggcgcac
    31321 gaaaccgggt agccaatgcc cgccgtttac ttccagaggc tgatttttgg gtggcgattg
    31381 aagctggcat tgatggcgac agcactttca gttgggtggt gattgaaaac accacccagc
    31441 gcggcgaggc gcgttcggca actttgccgt tgcctgcggt gatattggaa aaagtacgtg
    31501 aaggtgaggc gcttggtccc gtgatgtcgc gttataccgg tattgatgag attggtcgta
    31561 aggaaggggc gatcggcgtg tttaccgccg ggaaactcac tcgcgccagc gtctatcacc
    31621 aggcggtgat cctggcgctg agtccgtttc ataatgcggt gtattaagcg cctgatgcga
    31681 cgctgccgcg tcttatcatg cctaccaaac atattgaaat tacgggtatt tgtaggacgg
    31741 ataaggcgtt cacgccgcat ccggcattcg gtgcacgatg cctgatgcgc cacgtcttat
    31801 caggcctaca aaatcaatcg cttttcagca acacctcttc cagccactgg cgcagctcca
    31861 cgggcgcggc tttcaggctg ttagatccac gcgtaatcgt cgcgatgcct gcgccgagtt
    31921 cattttttaa ctcacgctgg ctcatttcgc cgcgcaacag ctcttcgaca atacgcacgc
    31981 gagtccccaa cgcttcgcgc tcatctggcg tcagcatcag gtttaacaac ggtaaatgga
    32041 gatcgttttg gtaggcattc ttaagcaggt cgacaaaacg taaccactcc tggtgacgct
    32101 gttctgccat cgctgctgaa tagggtgatt gttgggccat aatatgtcgc cattgttagc
    32161 gggggaagca aaatgcctcc cccggttgta ctcgctaaag agtacgatag catatcataa
    32221 acgtgcggat cagtaacgac gtccccattc cgtggcgctc atcaacgtcg gtttatcccc
    32281 catgaaatag cggtagtaag cgtcataagc cagcacgttc ttcacataac cgcgcgtctc
    32341 ggagaacgga atactctcga caaatgccac agcgtcgata cgcccggcgc tgttgccaag
    32401 ccaggttcgc acccgtccag gtccggcgtt ataagctgct gaggagaaaa tacggttatt
    32461 accaaattgc tgataaacat attgcaggta actggtgcca atgttgatat tcgtttccgg
    32521 atccagcaat tgcccagggc tgctataacc aggaatagag aacatcttca ccgtatgggt
    32581 cgccgtgccc ggcataatct gcatcaggcc gctggcccct accggtgatt tcactttcgg
    32641 attccaggcg ctctcctgac gggcaatcgc catcgcatag ctttgcggga tctccttccc
    32701 actggtgtaa cgtttgaaaa gatcgttgta agccagcggg aatcgctctt ccagatgatc
    32761 ccaaagcttc ccggcgatcg ttgcctgaac gctaagatcc caccattggt tgttgaaagc
    32821 ataccgcgcc agttgagcct gctctgtttt tgacttgctc ttcaccagat tggcccactc
    32881 gctacgcgcg gtgttatcga gattccagta catcaactcg cgcacgcgcg ccatctccgg
    32941 cccctgagtc agggcgctgt caacattctg cggcgcttta tcaatcttca gctcatactc
    33001 ttcgccgatg cgttgtgcag caaccatcgg gtagaaacca cgctgttgca tgagttgatg
    33061 caaaatctct tttgcttcag cttcacgtcc gcgttccagc aataaatccg cctgccagta
    33121 acgccattca tctttctctt tcgcttccat aggcagacgc gccagccagg tattcaggcc
    33181 gcgacgatcg ccagtgccaa gcgccattcg cacacggcgt tcaataagcg aagtagattg
    33241 cgagcgcata atggcgtcat cgcgccattt cgcctgctcg tcggtgacat cgttgcccat
    33301 caaacgccag gcgacgatat cgcgcagctc ctgaatctga tcttcattaa gctgctgcgc
    33361 ctgggcaagc gatgggatca tcagccgtgc attttcagca tcctgccgcg ccacactggc
    33421 aaacgccacc gccgccattt gacgggtaaa atcggtcgct ccggttgtac gcgcgaaggt
    33481 cagtaccgta ttggggttgt tcgccagtga aatgattgcc gaggcgatag tctggtaatc
    33541 ggcaggcatc tgccctgcca gcactgttac caggccagtg ttacccgctt tcatcgccag
    33601 acggatacgc tctaaatacg ccagcggatc ttgtttacct gacgcacgcc agacgctaaa
    33661 taacttgtca caggcgttag gctggctctt gccggttagc cacagctctt tcgccccttg
    33721 ccaggcttct tcactctgcc cggtgttcca tttcgcatag tagtaattac attgtgcttc
    33781 ggtagttccg ggcttttccg ggctaaaggc taacaggcca cgccagtctt cacgccgcgc
    33841 cagttcattg acgaaacgag attgcagcgt gcgagcggga ggaagcgtgg ggttagcgcg
    33901 aacaaagtta gtgaccgtca ccgccggttg attcatcagg tcatcggtga tctggcggta
    33961 ttccaggtag ggataaagcg gataatcctt cagtccaggc atcatttgtt cgaccacatc
    34021 catttgtcga ttatcccagg cctgcttgat ttgcgcgtaa cgactacgct gctcatccag
    34081 tgagtcggct cgcgccacgc tgctgaccgt cagcagacag acaccggcag ccaacagccg
    34141 ccaggtaact tgtttggctt tttccacaag cgcatcctct aagtgtaaat acatcaatgc
    34201 agcgtcatgc cgcgtaatag atttatgcta accagtcatt gccgtttacg ccacgttacg
    34261 gacacttttt tacttttact gcgagggtga tcgaccggct gggattaggc ggggaaaaag
    34321 gctacacttc gcctttgaaa actcatcatc acgataaaca tagaggcgaa gtccaacgtg
    34381 gctcaattcg tttataccat gcatcgtgtc ggcaaagttg ttccgccgaa acgtcatatt
    34441 ttgaaaaaca tctctctgag tttcttccct ggggcaaaaa ttggtgtcct gggtctgaac
    34501 ggcgcgggta agtccaccct gctgcgcatt atggcgggca ttgataaaga catcgaaggt
    34561 gaagcgcgtc cgcagccaga catcaagatt ggttatctgc cgcaggaacc gcagctgaac
    34621 ccggaacaca ccgtacgtga gtccattgaa gaagcggttt cagaagtggt taacgccctg
    34681 aaacgcctgg atgaagtgta tgcgctgtac gccgatccgg atgccgattt tgacaagctg
    34741 gccgctgaac aaggccgtct ggaagagatc attcaggctc acgacggtca taatctgaac
    34801 gtacagctgg agcgtgcggc ggatgcgcta cgtctgccgg actgggacgc gaaaatcgct
    34861 aacctctccg gtggtgagcg tcgtcgcgtg gcgttgtgcc gcctgctgct ggaaaaacca
    34921 gacatgctgc tgctcgacga accgaccaac cacctggatg ccgaatccgt ggcctggctg
    34981 gaacgcttcc tgcacgactt cgaaggcacc gtggtggcga ttacccacga ccgttacttc
    35041 ctcgataacg ttgcgggctg gatcctcgaa cttgaccgcg gtgaaggtat tccgtgggaa
    35101 ggtaactact cctcctggct ggagcagaaa gatcagcgtc tggcgcagga agcttcacaa
    35161 gaagcggcgc gtcgtaagtc gatcgagaaa gagctggagt gggtacgtca gggaactaaa
    35221 ggccgccagt cgaaaggtaa agcacgtctg gcacgctttg aagaactgaa cagcaccgaa
    35281 tatcagaaac gtaacgaaac caacgaactg tttattccac ctggaccgcg tctgggcgat
    35341 aaagtgctgg aagtcagcaa cctgcgtaaa tcctatggcg atcgtctgct gattgatgac
    35401 ctgagcttct cgatcccgaa aggagcgatc gtcgggatca tcggtccgaa cggtgcgggt
    35461 aaatcgaccc tgttccgtat gatctctggt caggaacagc cggacagcgg caccatcact
    35521 ttgggtgaaa cggtgaaact ggcgtcggtt gatcagttcc gtgactcaat ggataacagc
    35581 aaaaccgttt gggaagaagt ttccggcggg ctggatatca tgaagatcgg caacaccgag
    35641 atgccaagcc gcgcctacgt tggccgcttt aactttaaag gggttgatca gggtaaacgc
    35701 gttggtgaac tctccggtgg tgagcgcggt cgtctgcatc tggcgaagct gctgcaggtt
    35761 ggcggcaaca tgctgctgct cgacgaacca accaacgacc tggatatcga aaccctgcgc
    35821 gcgctggaaa acgccctgct ggagttcccg ggctgtgcga tggttatctc gcacgaccgt
    35881 tggttcctcg accgtatcgc cacgcacatt ctggattacc aggatgaagg taaagttgag
    35941 ttcttcgaag gtaactttac cgagtacgaa gagtacaaga aacgcacgct gggcgcagac
    36001 gcgctggagc cgaagcgtat caagtacaag cgtattgcga agtaatgcgt aaaatgccgg
    36061 atgcggcgcg aacgccttat ccggcctaca aaaccagcaa tttcaatacg ttgtaaaact
    36121 gtagacctga taagacgcgg taagcgtcgc atcaggcatt gagcaccgaa tgccggatgc
    36181 ggcgtgaaca ccttttccgg tctacaacag ccttagcatc ctcaatttcg tggcgggcca
    36241 cccgtgcagg ttttttcaaa ccttgtttaa tctcgacagc ctcttccaga gaggttttca
    36301 actcatcaaa aaaactcatt ttatacctcg cctttgagca gtttcatcac ggttatctct
    36361 gctcccccat catctcccgc accagctcca cgcagcgcag gaaacggctg tcgtaatctt
    36421 cctcttcaac ccgcacaaat tcgatattgt tctcttccag catctccacc agcagattct
    36481 ggaactcttt acgatccacc gaactgccga ggctgcgtaa accatccacc acccacggcg
    36541 tgttgttctc cagcaggatc accagatcga aacggtattc atcaatcagc gcctgcacga
    36601 acggatgttc ccgcccttcg tactttttgc agaacgcctg agtggtgaca aaatcggtat
    36661 cgataaatgc cactttattg gcatatttca ccgcaaaatc aatgtattga gcgtggccca
    36721 gcgcgatttt atcgtagtca gaatactgca atgcgatctc atcaccgccg aggtgtgaaa
    36781 agacataatc gcggccatat tcccacgcac tggtggtgtt gaagatattg gcaagtttgt
    36841 ttaccagggt ggatttaccg ctcgactcgc cgccaaggat cgccacggta cgcacaaaaa
    36901 acggcttcac ttcggtagga atatattccc agtagcggaa cgggttttcg cggatctgcg
    36961 caccgctgat actcataaag gtacgtttcg gatcgaccag caccgtctcg atccccagat
    37021 gttccatata ctgtggcgca tcggcttctt ccgaggtgta gatcagatcc ggctggatcc
    37081 ccttttcagc cataaacttt ttgataccgt tgctccacac atcccagccg tgcggatacg
    37141 gctccatgcc ctcttcgttg aaagcatgaa tgcgaatatt tttctgatat ttaaaagttt
    37201 gcaataacca gcgcagacga tccggcacgg tcggctgctg cgacatggca ctgtcttcga
    37261 acaacgcgcg gtcacgggta tcgtcaaaac ccataatgat atgcagctcg tcaacctggc
    37321 tacaggcgcg ctggataagg tagatatgtc cggtatgcag tgggtagaac ttaccgaata
    37381 cgacaccgat cgttttcttc tgccggggaa actcaagccc caaaaaacgg tgcaacgcct
    37441 ccagcttttg cgcgctgggg cttttgattt tggcattcag taactggctt aaataccctt
    37501 tggtcatacc gctggcatca gctacctgct gtagcgtgca gccctgttgc ttgatggcag
    37561 ttttcaggta atcaaatgac gacatatctc cctccgtatt tctcattata agtcgtcgaa
    37621 cacgctaagc gcgtcggaga gttttttaac gccaaaaatc tgcatccctt ccggcgcttt
    37681 tttcggtacg ttagccgccg gaacaatcgc ccggcgaaaa ccgtgtttcg ccgcttcaga
    37741 gattcgttcc tgaccgctgg gcaccgggcg gatctcccct gccagcccga cttcaccaaa
    37801 caccaccaga tcctgcggta gcggtctgtc acgcaggctg gaaaccatcg ccagcagtaa
    37861 cgctaagtcg gcgctggttt cggttacctt tacgccgccg accacgttca caaacacatc
    37921 ctgatcggcc atttgcagac caccgtgacg gtgcaagaca gccagcagga ttgccagacg
    37981 gttttgttcc agccccactg ccacgcggcg cggattcgcc atcatcgagt gatcgaccag
    38041 cgcctgaatc tccaccagca acggacgcgt tccttcccac accaccatca cggagctgcc
    38101 ggaggtcact tcatctccgc gacttaagaa aattgccgaa gggttgctga cttcacgcag
    38161 cccctgttcg gtcatcgcga agacgcccag ctcattcacc gcgccgaagc ggtttttatg
    38221 gctgcgcaag gtgcgaaaac gggagtcggc atcgccatct aaaagcaccg aacagtcgat
    38281 acagtgttcc agcactttcg ggccagccag cgaaccatct ttggttacgt gccccaccat
    38341 gacaatcgcc acaccgcgcg ttttggcgaa gcgtgtcaaa taagccgccg tttcacgcac
    38401 ctgcgccacg ctaccaggcg atgactgtac atccgccata tgcatcacct ggatcgagtc
    38461 aattaccatc agcttcggtt gctcttcttc ggcaatcagg cagatctgtt cgatgctggt
    38521 ttccgacaac atattgagat tgtcagtcgg caggccaagg cgatgagcgc gcattgccac
    38581 ctgttgcagc gactcttcgc cggtgacata cagcgttttc atctgctggg ccagtttgca
    38641 cagcgtttgc agtagcagcg tggatttccc cgcaccaggg ttaccgccaa tcagaatggc
    38701 acttcctggc accacgccgc cgcctagtac gcggtcgaac tctttaaatc cggtggaaaa
    38761 acgcggcagc tcttcaaggc tgatatcgga gagtttctgg acttttgcca ccccggcgct
    38821 accggcatag ccgctgagac gctcgttacg cgccaccgta ggcgacgcag caagacgcac
    38881 ctcggtgatg gtgttccagg catgacaggc actgcactgc ccctgccagc gcggataatc
    38941 ggccccgcat tcattacaaa caaaggcgcg ttttggagct tttgccacgg tgtacctcgt
    39001 taatgctgtg ccgcccgcag gatggcgggc gagcaattac ttctgattca ggctgcctga
    39061 gaggatgcag aataccccca tcaggtcagc gtgacggatg gtgacttccg ccttttcatt
    39121 cacttttggc ttggcatggt aggcaatccc cagccctgcc gctttgatca tcggcaggtc
    39181 attggctcca tcgccaatcg ccacggtctg cgccagcggg atttcatact cctgcgcgag
    39241 gcgagtcaga gttttcgctt tgtactgcgc gtctacgatg tcgccgatca cattgccggt
    39301 aaatttaccg tccatgatct ccagttcatt ggctaccacg gctgtcaggc gcagcttgtc
    39361 gcgcaggtat tcagcaaaga aagtaaagcc gccggaggca atcgccactt tccagcccag
    39421 cgtttccagc ttgagcacca gttgcgttaa gcctggcatc agcggcagat tttcacgcac
    39481 ctgttgcaga atattggcgt cagcgccttt cagcgtcgcc acgcggctgc gcaggctggc
    39541 ggtaaaatcg agttcgccgc gcatcgcccg ttcggttact tccgccacca tctcgcccgt
    39601 tccggccagt ttggcaattt catcaataca ttcaatctgg atggcggtgg aatccatgtc
    39661 catcaccagc aaacccggcg tgcgcaggtg cgggattttt cccagcggcg cgacatccag
    39721 ctgcgcttcg tgggccaggc gtgtagcccg tgcggtgagt gaacctgcca gacgaatcac
    39781 ctgataatct tccacgcacc aggcggcaac aatcaccatc gccgcaccca gtttgctctg
    39841 gtattgggtc agacgctgtt tatccagccc acgaccatac agcagccagc cgctacgacc
    39901 tgcgtggtaa tccagtggca tcacttcatc accacttaat gaaagaggca gacccggcca
    39961 taaagagaca tcttcaggca ggtcgcacca ggtaatgtta ggcattaagg ctcctgtaaa
    40021 atcgttcgaa gcagggaaaa taacgcatga ggctaccttg tatccattgc ttctggcaac
    40081 attaagtctc aaattttcaa agggtggaag atggctcgca caaaactgaa attccggctg
    40141 catcgggcag tgattgtcct gttctgtctt gccttgttag tggcactgat gcagggggcg
    40201 tcatggttta gtcaaaacca ccagcgacag cgtaatccac agctggaaga actggcccgc
    40261 cccctggcgc gtcaggtgac gctgaacgtt gcaccgctga tgcgtaccga ctcaccggat
    40321 gaaaaacgca ttcaggcgat cctcgatcag ttaacggatg aaagccgtat cctcgacgcg
    40381 ggtgtgtatg acgaacaagg cgatcttatc gcacgttctg gcgaaagcgt cgaagtgcgc
    40441 gatcggctgg cgctcgacgg taaaaaagca ggcggctatt ttaaccagca gattgtcgag
    40501 ccaattgccg ggaaaaacgg accgctcggc tatctgcgcc tgacactcga cacccatacg
    40561 ctcgccaccg aagcccaaca ggtggataac accactaaca ttttacgcct gatgttgctg
    40621 ctctcactgg caatcggcgt agtgctgacc cgcacgctgc tacagggtaa acgcacccgc
    40681 tggcagcaat cgcccttcct gttaaccgcc agcaaaccgg tgccggaaga ggaagaaagc
    40741 gagaaaaaag agtgccccat tactacaaga aaggaaatcg ttatgtccac attacgcctg
    40801 ctcatctctg actcttacga cccgtggttt aacctggcgg tggaagagtg tatttttcgc
    40861 caaatgcccg ccacgcagcg cgttctgttt ctctggcgca atgccgacac ggtagtaatt
    40921 ggtcgcgcgc agaacccgtg gaaagagtgt aatacccggc ggatggaaga agataacgtc
    40981 cgcctggcgc ggcgcagtag cggtggcggc gcggtgttcc acgatctcgg caatacctgc
    41041 tttaccttta tggctggcaa gccggagtac gataaaacca tctccacgtc gattgtgctc
    41101 aatgcactga atgcgctcgg cgtcagtgcc gaagcgtccg gacgtaacga tctggtggtg
    41161 aaaaccgccg aaggcgaccg caaagtctca ggatcggcct atcgcgaaac caaagatcgt
    41221 ggcttccacc acggcacctt gctgctcaat gccgacctta gccgcctggc aaactatctc
    41281 aatccggata aaaagaaact ggcggcgaaa ggcattactt cagtgcgttc ccgcgtgacc
    41341 aacctcaccg agctgctgcc ggggatcccc catgagcagg tttgcgaggc cataaccgag
    41401 gcctttttcg cccattatgg cgagcgcgtg gaagcggaaa tcatctcccc ggacaaaacg
    41461 ccagacttgc caaacttcgc cgaaaccttt gcccgccaga gtagctggga gtggaacttc
    41521 ggtcaggctc cggcattctc gcatctgctg gatgaacgct ttagctgggg cggcgtggaa
    41581 ctgcatttcg acgttgaaaa aggccatatc acccgcgccc aggtgtttac cgacagcctc
    41641 aaccccgcgc cgctggaagc cctcgccggg cgactgcaag gctgcctgta ccgcgcggat
    41701 atgctgcaac aagagtgcga agcgctgttg gttgacttcc cggaccagga aaaagagcta
    41761 cgggagttgt cgacgtggat agcgggggcg gtaaggtaac tcgctgatta aaatatccaa
    41821 ggaaggaaat atgatcgatc tggaaaacca ggaacgggag attatcaatt taatgttcag
    41881 ccagcgtatt tcctggctag ccgccgtgcg tatcaggcat aaattatcgc ttgctgaagt
    41941 aagtaaaatg ttaggtatct cgattaattc tctaaaacag atcgaaaaga cggaacggct
    42001 tagctcaaac atcaaaagca aaatggcaga aatttatggt tgtccaccag agttgctgat
    42061 atgcccttcc tggatgacgg ctgaacataa atgaatagat tcgtgaatag atacttgaat
    42121 ggatt
//
LOCUS       DAZFAB010000030        41151 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZFAB010000030 DAZFAB010000000
VERSION     DAZFAB010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41151
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.030"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(105..368)
                     /gene="cedA"
                     /locus_tag="ACHJWA_001903"
     CDS             complement(105..368)
                     /gene="cedA"
                     /locus_tag="ACHJWA_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFO4308511.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            551..2812
                     /gene="katE"
                     /locus_tag="ACHJWA_001904"
     CDS             551..2812
                     /gene="katE"
                     /locus_tag="ACHJWA_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFO4308512.1"
                     /translation="MSQQNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTASGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGSRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(2859..3617)
                     /gene="chbG"
                     /locus_tag="ACHJWA_001905"
     CDS             complement(2859..3617)
                     /gene="chbG"
                     /locus_tag="ACHJWA_001905"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFO4308513.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMNFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSHGFSSAFYGEEISEALFLQVLDDSSHRGERSLEVMCHPAFVDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(3630..4982)
                     /gene="celF"
                     /locus_tag="ACHJWA_001906"
                     /gene_synonym="chbF"
     CDS             complement(3630..4982)
                     /gene="celF"
                     /locus_tag="ACHJWA_001906"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFO4308514.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCTLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5088..5930)
                     /gene="chbR"
                     /locus_tag="ACHJWA_001907"
     CDS             complement(5088..5930)
                     /gene="chbR"
                     /locus_tag="ACHJWA_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFO4308515.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(5938..6288)
                     /gene="chbA"
                     /locus_tag="ACHJWA_001908"
     CDS             complement(5938..6288)
                     /gene="chbA"
                     /locus_tag="ACHJWA_001908"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFO4308516.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6339..7697)
                     /gene="chbC"
                     /locus_tag="ACHJWA_001909"
     CDS             complement(6339..7697)
                     /gene="chbC"
                     /locus_tag="ACHJWA_001909"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFO4308517.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVVALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
     gene            complement(7782..8102)
                     /gene="chbB"
                     /locus_tag="ACHJWA_001910"
     CDS             complement(7782..8102)
                     /gene="chbB"
                     /locus_tag="ACHJWA_001910"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFO4308518.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(8401..8739)
                     /gene="osmE"
                     /locus_tag="ACHJWA_001911"
     CDS             complement(8401..8739)
                     /gene="osmE"
                     /locus_tag="ACHJWA_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFO4308519.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            8941..9768
                     /gene="nadE"
                     /locus_tag="ACHJWA_001912"
     CDS             8941..9768
                     /gene="nadE"
                     /locus_tag="ACHJWA_001912"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFO4308520.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREACIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPQQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            9998..10885
                     /gene="cho"
                     /locus_tag="ACHJWA_001913"
     CDS             9998..10885
                     /gene="cho"
                     /locus_tag="ACHJWA_001913"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFO4308521.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(10845..11420)
                     /gene="ves"
                     /locus_tag="ACHJWA_001914"
     CDS             complement(10845..11420)
                     /gene="ves"
                     /locus_tag="ACHJWA_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFO4308522.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADHFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(11623..12108)
                     /gene="spy"
                     /locus_tag="ACHJWA_001915"
     CDS             complement(11623..12108)
                     /gene="spy"
                     /locus_tag="ACHJWA_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFO4308523.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(12438..13406)
                     /gene="astE"
                     /locus_tag="ACHJWA_001916"
     CDS             complement(12438..13406)
                     /gene="astE"
                     /locus_tag="ACHJWA_001916"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFO4308524.1"
                     /translation="MDNFLALTLTSKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFKKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(13399..14742)
                     /gene="astB"
                     /locus_tag="ACHJWA_001917"
     CDS             complement(13399..14742)
                     /gene="astB"
                     /locus_tag="ACHJWA_001917"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFO4308525.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNKASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVVRQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTQEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(14739..16217)
                     /gene="astD"
                     /locus_tag="ACHJWA_001918"
     CDS             complement(14739..16217)
                     /gene="astD"
                     /locus_tag="ACHJWA_001918"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFO4308526.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVGQACR
                     AARAAFPRWARLSFAERQAVVERFAGLLERNKGELTAIIARETGKPRWEAATELTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGFPPGVLNLVQGGCETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRVLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVFTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     IRMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(16214..17248)
                     /gene="astA"
                     /locus_tag="ACHJWA_001919"
     CDS             complement(16214..17248)
                     /gene="astA"
                     /locus_tag="ACHJWA_001919"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFO4308527.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLVRTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(17245..18465)
                     /gene="astC"
                     /locus_tag="ACHJWA_001920"
     CDS             complement(17245..18465)
                     /gene="astC"
                     /locus_tag="ACHJWA_001920"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFO4308528.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNESVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECASVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNIINHRYGLFNEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAVACEHFVSRGSS"
     gene            18911..19717
                     /gene="xthA"
                     /locus_tag="ACHJWA_001921"
     CDS             18911..19717
                     /gene="xthA"
                     /locus_tag="ACHJWA_001921"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFO4308529.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDEEEAQRRIIMAEIPSPLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRENPVLIMGDMNISPGDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMERLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            19884..20594
                     /gene="ydjX"
                     /locus_tag="ACHJWA_001922"
     CDS             19884..20594
                     /gene="ydjX"
                     /locus_tag="ACHJWA_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFO4308530.1"
                     /translation="MNAERKFLFACLIFALAIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPFLGTLLSLIAATLASSCSFLLARW
                     MGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRNPLTHPKNEG"
     gene            20599..21276
                     /gene="ydjY"
                     /locus_tag="ACHJWA_001923"
     CDS             20599..21276
                     /gene="ydjY"
                     /locus_tag="ACHJWA_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFO4308531.1"
                     /translation="MLQHYSVSWKKGLTALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMGYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKTYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPANNTLAT
                     VTFKITE"
     gene            21291..21998
                     /gene="ydjZ"
                     /locus_tag="ACHJWA_001924"
     CDS             21291..21998
                     /gene="ydjZ"
                     /locus_tag="ACHJWA_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFO4308532.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGSLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            21998..22546
                     /gene="ynjA"
                     /locus_tag="ACHJWA_001925"
     CDS             21998..22546
                     /gene="ynjA"
                     /locus_tag="ACHJWA_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFO4308533.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSSDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQELTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            22556..23722
                     /gene="ynjB"
                     /locus_tag="ACHJWA_001926"
     CDS             22556..23722
                     /gene="ynjB"
                     /locus_tag="ACHJWA_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO4308534.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTDWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALNEAPNDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTFRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            23695..25230
                     /gene="ynjC"
                     /locus_tag="ACHJWA_001927"
     CDS             23695..25230
                     /gene="ynjC"
                     /locus_tag="ACHJWA_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFO4308535.1"
                     /translation="MATPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCTRLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDKLGIGLGFTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLGYLLWCTWRRTIPRVDGVRKP
                     ATPLLPGITLASFLPLTSVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNMDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRAKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAALALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            25230..25883
                     /gene="ynjD"
                     /locus_tag="ACHJWA_001928"
     CDS             25230..25883
                     /gene="ynjD"
                     /locus_tag="ACHJWA_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO4308536.1"
                     /translation="MLCVKNVSLRLPESCLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            25950..27257
                     /gene="ynjE"
                     /locus_tag="ACHJWA_001929"
     CDS             25950..27257
                     /gene="ynjE"
                     /locus_tag="ACHJWA_001929"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFO4308537.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRSSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNGKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAARGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAAARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWNNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(27266..27886)
                     /gene="ynjF"
                     /locus_tag="ACHJWA_001930"
     CDS             complement(27266..27886)
                     /gene="ynjF"
                     /locus_tag="ACHJWA_001930"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO4308538.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPQQNALAGGWLLFAFIGTGSSFLAFAALAAKYQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            27973..28380
                     /gene="nudG"
                     /locus_tag="ACHJWA_001931"
     CDS             27973..28380
                     /gene="nudG"
                     /locus_tag="ACHJWA_001931"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFO4308539.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            complement(28346..28618)
                     /gene="ynjH"
                     /locus_tag="ACHJWA_001932"
     CDS             complement(28346..28618)
                     /gene="ynjH"
                     /locus_tag="ACHJWA_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFO4308540.1"
                     /translation="MSRALFAVVLAFPLIALANPHYGPDVEVNVPPEVVSSGGQSAQP
                     CTQCCVYQDKNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            28854..30197
                     /gene="gdhA"
                     /locus_tag="ACHJWA_001933"
     CDS             28854..30197
                     /gene="gdhA"
                     /locus_tag="ACHJWA_001933"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFO4308541.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKSSRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(30314..31354)
                     /gene="ynjI"
                     /locus_tag="ACHJWA_001934"
     CDS             complement(30314..31354)
                     /gene="ynjI"
                     /locus_tag="ACHJWA_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO4308542.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMIKENKTMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLEEQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANHSSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(31482..33443)
                     /gene="topB"
                     /locus_tag="ACHJWA_001935"
     CDS             complement(31482..33443)
                     /gene="topB"
                     /locus_tag="ACHJWA_001935"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFO4308543.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRYLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLISRQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLIKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            complement(33448..34491)
                     /gene="selD"
                     /locus_tag="ACHJWA_001936"
     CDS             complement(33448..34491)
                     /gene="selD"
                     /locus_tag="ACHJWA_001936"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFO4308544.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(34608..35159)
                     /gene="ydjA"
                     /locus_tag="ACHJWA_001937"
     CDS             complement(34608..35159)
                     /gene="ydjA"
                     /locus_tag="ACHJWA_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFO4308545.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            35320..37176
                     /gene="sppA"
                     /locus_tag="ACHJWA_001938"
     CDS             35320..37176
                     /gene="sppA"
                     /locus_tag="ACHJWA_001938"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFO4308546.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKADKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKAKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            37292..38002
                     /locus_tag="ACHJWA_001939"
     CDS             37292..38002
                     /locus_tag="ACHJWA_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310500.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HFO4308547.1"
                     /translation="MNKAPSLIAAIVLGLGISACGYFVGDGVKHLKTNNRYVNVRGLS
                     EKEVRADTAELTIAINFKGNVPGELFPKLEEAQKKIVAELNAQGINEKEIILGQWTSK
                     RTDSFYLKDDPTMPRYNADGSVTIKTHNVAAVEKVVAKLNELQVATDGAIAESKVAYR
                     FNGIGALRAEMIAAATKDARNAALQFATDSGSQVGSISDASQGVFQIFASGSDEDDPT
                     AINKTVRVVTTVTYALQD"
     gene            38134..39150
                     /gene="ansA"
                     /locus_tag="ACHJWA_001940"
     CDS             38134..39150
                     /gene="ansA"
                     /locus_tag="ACHJWA_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFO4308548.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            39161..39802
                     /gene="pncA"
                     /locus_tag="ACHJWA_001941"
     CDS             39161..39802
                     /gene="pncA"
                     /locus_tag="ACHJWA_001941"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFO4308549.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(39895..>41151)
                     /gene="ydjE"
                     /locus_tag="ACHJWA_001942"
     CDS             complement(39895..>41151)
                     /gene="ydjE"
                     /locus_tag="ACHJWA_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4308550.1"
                     /translation="GFLSYSGNVVLAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGG
                     FIGDYFGRRRAFRINLLIVGIAATGAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFT
                     EFIPATVRGKWSARLSFVGNWSPMLSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSG
                     KYFIESPRWLAGKGQIAGAECQLREVEQQIEREKSIRLPPLTSYQSNSKVKVIKGTFW
                     LLFKGEMLRRTLVAITVLIAMNISLYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAP
                     VGIFIAALIIDHFPRRLFGSTLLIIIAVLGYIYSIQTTEWAILIYGLVMIFFLYMYVC
                     FASAVYIPELWPTHLRLRGSGFVNAVGRIVAVFTPYGVAALLTHYGSITVFMVLGVML
                     LLCALVLSIFGIETRKVSLEEISEVN"
BASE COUNT        10192 a        10842 c        10143 g         9974 t
ORIGIN      
        1 cgtcaaaggc agctttttgt tacaggtggt ttgaatgaat gtagcaatga aatagagaat
       61 ttcaggccat gtaactcccg gcaaaaccgg gaggtacgta atctttactc agtcacttcc
      121 ccttcctggc ggatctgatt tgcccaacgc tgggcggatt caggcacagt aaacgccggt
      181 gagcgcagaa atgactctcc catcagtaca aacgcaacat atttgccacg cagcatccag
      241 acatcacgaa acgaatccat ctttatcgca tgttctggcg gcgcgggttc cgtgcgtggg
      301 acatagctaa taatctggcg gttttgctgg cggagcggtt tcttcattac tggcttcact
      361 aaacgcatat taaaaatcag aaaaactgta gtttagccga tttagcccct gtacgtcccg
      421 ctttgcgtgt atttcataac accgtttcca gaatagtctc cgaagcgggg tctggctggt
      481 ggtctatagt tagagagttt tttgaccaaa acagcggccc tttcagtaat aaattaagga
      541 gacgagttca atgtcgcaac aaaacgaaaa gaacccacat cagcaccagt caccactaca
      601 cgattccagc gaagcgaaac cggggatgga ctcactggca cctgaggacg gctctcatcg
      661 tccagcggct gaaccaacac cgccaggtgc acaacctacc gcctcaggga gcctgaaagc
      721 ccctgatacg cgtaacgaaa aacttaattc tctggaagac gtacgcaaag gcagtgaaaa
      781 ttatgcgctg accactaatc agggcgtgcg catcgccgac gatcaaaact cactgcgtgc
      841 cggtagccgt ggtccaacgc tgctggaaga ttttattctg cgcgagaaaa tcacccactt
      901 tgaccatgag cgcatcccgg aacgtattgt tcatgcacgt ggatcagccg ctcacggtta
      961 tttccagcca tataaaagct taagcgatat caccaaagcg gatttcctct cagatccgaa
     1021 caaaatcacc ccagtatttg ttaggttctc taccgttcag ggtggtgctg gctctgccga
     1081 taccgtgcgt gatatccgtg gctttgccac caagttctat actgaagagg gtatttttga
     1141 cctcgttggc aataacacgc caatcttctt tatccaggat gcgcataaat tccccgattt
     1201 tgttcatgcg gtaaaaccag aaccacactg ggcaattcca caagggcaaa gcgcccacga
     1261 tactttctgg gattatgttt ctctgcaacc tgaaactctg cacaacgtga tgtgggcgat
     1321 gtcggatcgc ggcatccccc gcagttaccg caccatggaa ggctttggta ttcatacctt
     1381 ccgcctgatt aatgccgaag ggaaggcaac gtttgtacgt ttccactgga aaccactggc
     1441 aggtaaagcc tcactcgttt gggatgaagc acaaaaacta accggacgtg acccggactt
     1501 ccaccgccgc gagttgtggg aagccattga agcaggcgat tttccggaat acgaactggg
     1561 cttccagttg attcctgaag aagatgaatt caagttcgac ttcgatcttc tcgatccaac
     1621 taaacttatc ccggaagaac tggtgcccgt tcagcgtgtc ggcaaaatgg tgctcaatcg
     1681 caatccggat aacttctttg ccgaaaacga acaagcagca ttccatccag gtcatattgt
     1741 tcctggtctg gacttcacca acgatcccct gttgcaggga cgtttgttct cctataccga
     1801 tacacaaatc agtcgtcttg gtgggccgaa tttccatgag attccgatta accgcccaac
     1861 ctgcccttac cataatttcc agcgtgacgg catgcatcgt atggggatcg acactaaccc
     1921 ggcgaattac gaaccgaact cgatcaacga taactggccg cgcgaaacac cgccgggacc
     1981 gaaacgcggc ggttttgaat catatcagga acgcgtggaa ggaaataaag ttcgcgagcg
     2041 cagtccatct tttggcgaat attattccca tccgcgtctg ttctggctaa gtcagacgcc
     2101 attcgagcag cgccatattg tcgatggttt cagttttgag ttaagcaaag tcgttcgtcc
     2161 gtatattcgt gagcgcgttg ttgaccagct ggcgcatatt gatctcactc tggcccaggc
     2221 ggtggcgaaa aatctcggta tcgaactgac tgacgaccag ctgaatatca ccccacctcc
     2281 ggacgtcaac ggtctgaaaa aggatccatc cttaagtttg tacgccattc ctgacggtga
     2341 tgtgaaaggt cgcgtggtag cgattttact taatgatgaa gtgagatcgg cagaccttct
     2401 ggccattctc aaggcgctga aggccaaagg cgttcatgcc aaactgctct actcccgaat
     2461 gggtgaagtg actgcggatg acggtacggt gttgcctata gccgctacct ttgccggtgc
     2521 gccttcgctg acggtcgatg cggtcattgt cccttgcggc aatatcgcgg atatcgctga
     2581 caacggcgat gccaactact acctgatgga agcctacaaa caccttaaac cgattgcgct
     2641 ggcgggagac gcgcgcaagt ttaaagcaac aatcaaggtc gctgaccagg gtgaagaagg
     2701 gattgtggaa gctgacagcg ccgacggtag ttttatggat gaactgttaa cgctgatggc
     2761 agcacaccgc gtgtggtcac gcattcctaa gattgacaaa attccggcgt aaatatggat
     2821 aaggtcacgt cccctgggga gtgaccttac caccttactt acacatcaag ataactaccc
     2881 aaccgataac cacgctcagc aattgcatat ttcaacgacg ctgatgtcag cacttccagc
     2941 tccgttaacc gtgggaaaca gtaagcactc tggcgaatcg tattatcgac aaacgccgga
     3001 tggcacatga cttccagcga gcgttccccg cgatggctgg aatcatcaag cacttgcagg
     3061 aacagtgcct cgctaatctc ttcaccataa aatgcgctac tgaatccgtg cgaactgcgc
     3121 agattagccg gtaaatcacc agcgttagat agcggctgac ggtcgatgcg caacgcaatc
     3181 ccctcttcag ccgcaaacct ggcaacaata gggaaaatct gcgggaacat atgcacatga
     3241 tgatggctat caagatgcgt aggtttgcgt ccaaatagct caatgaaacg caaatactga
     3301 ctggcaagct cctgagtaat ttcttccagc ggtaaagcat cttcttctgc caactgccag
     3361 atccattttc ccagcacacc atcgcgggtt aaccccggca tagctgtcag tggcttgccc
     3421 atagtaagga caaagttcat ccctatggcc agactcggtt catcacgact caactgcacc
     3481 gcatggtcaa tagcctgccc attcaccagc gccgtcgtcg atgtgacaat cccattgcga
     3541 caggcctcga taatgccgta gttctgacct ttgcttaagc caaaatcatc ggcattaaca
     3601 atcagtaagc gttccataat cagcctcggt taatgtgctt ttttaagctc tgcgatgcag
     3661 tcggcaaaat ttggcagcca tttctcgtgc gccagaatca tctcgcgtgc cagcagctca
     3721 gcatcgcgat cggaatgcac caacggacta aggtttagcg ccagtaacac atcgttaaat
     3781 tctccgctaa gtgcggcgtt gctggcagca atctcgaagc ctttaatggt gtgaatcagc
     3841 cccatcactt tatcatcgaa atgcgtaatg cgcggatgtg gcgtcgcgcc atcgcgcccc
     3901 agcgtacagg tcatttctac cgcccagtct gccggaatat tatcaatatg cccatgatgc
     3961 gggatattaa cgtaatgttc tgcttgctta tcgttgtaga tagcgttgat cacttcgcac
     4021 gctgcatcag agtaataagc cccaccgcgc tgttccagtt ctttcggctt aactttcaac
     4081 tccgggtttt tatacagctc aaaaagttgt ttctcgactt tctgtactac ctgcgctcgt
     4141 gcgccgcctt tgtagtattc gcccatttca atagccagca tctctttttg cttgaagtaa
     4201 taaagcaaat aagaacacgg caacagattc agagaacgaa ttaagccttc gctaaatggc
     4261 agatcgaaaa tatttttaac gccagatgct tttaactgcc cggatgccac accatcaagc
     4321 aattcggcaa agcgcgactt gccatttacc agcacatcct taatgaacac catatggttg
     4381 aggccgaaca gatcgataga taaatcatcg ctgtctttca gcatcagaac atcgcgaata
     4441 aacatcttca tgccgatcgg aatattacac acgccgataa agcgtttaaa tccggtatga
     4501 cgataaacgg cttcagtgac cattcccgcc gggttagtga agttaatcac ccatgcattc
     4561 ggacaaagtt cttcgacatc ttttacgatg tcaaaaatca ccggaatggt acgcagacct
     4621 ttaaacagac cgcccgcgcc gttggtttcc tgaccaagat aaccatgact taatggaata
     4681 cgttcatcca gttcacgcgc cggtaattgg ccaacgcgca gttgggtagt aacgaaatca
     4741 gcatctttca atgcttcgcg gcgatccagc gttttataaa gcttcatcgg gacgccagcg
     4801 ttatcaatca tccgttggca gagatcgaaa ataatatcca gtttagcttt gccaccttcg
     4861 acatccacca gccataattc gctgaccggc aattcgtgat aacgcttaat aaatccttcc
     4921 agtaactccg gggtatagct gctcccgcca ccaatagtga cgacttttaa tttctggctc
     4981 ataatttctc ccttcagtac agaatactga tatctggcat atctgccccc cccggacata
     5041 aataatccag caacaggaca gatatgtgaa ttgtcaggta taacgactta ctgattaaat
     5101 tcagtcaatt tcttacgata gctcttaggc gtaaaggacg ttaatttctt aaacgtttta
     5161 ataaacaaac tcgggctact ataaccggcc tcaaacgcaa tatccgtcac tgaatagttg
     5221 gtcatttcca gttgtttttt ggcaaaatta atacggattt cattaataat ctgcattggc
     5281 gttttgccat aatatcgttg agtcgcccgc gtcaaatatt cctgtgattt ggctgacaac
     5341 gtcaccatat tctccagcgc cgattcacta aactgctctt tatcatgcat cttttctacc
     5401 gtacttttca gccactgcgg tacatcatca atcacctgtt cttcgcggta atgacgtaaa
     5461 cggttaatga cataaaaagt aaccatctca acaaactctt ccagccctgt ttcgcggaaa
     5521 ttcaatgaag aaatcactgt ttccacatag gtgagaaaag cgttattggt ccggtatacc
     5581 tgcgaagcta caaagcaata aggcaacaat ggcaggtaat gctgctcaaa aaagcgttta
     5641 ctgatcccaa cgttcaatat gcgcgtggca ccaaactcat aaaaactttg atggtgcgaa
     5701 cctaacggaa taaaaacaaa atcgccccgt tccagtaaca cgcgcttacc gttaatctcc
     5761 tggaaataac gcccggttaa taccagagta aattcataat agtcgtgctg atgcagtccg
     5821 ctgatactct cagttttgtt atagataaac acatggaagt ttttgccatt aaacaactgc
     5881 tgttctcggg cagtggcaat ttccggcgcg ttaatcactg gctgcatcat cgactcctta
     5941 tgccttcagt ttttcatgaa gctcaattaa ttcagtaatc agttcacgcg caagcatgga
     6001 cgtcattaaa tgatcctgag cgtggaccag caccagactc actttcatct taccttcgcc
     6061 cgcatcgcct tcaatcagtt tcgtctgtac cagatgcgct tcattcaatg ccatccgtga
     6121 ctgatccatc atggcttttg ctgcggcaaa atcgccctgc ttcgcctgtt tcagtgcggc
     6181 atacgccagg ctgcgcgctt gtccggagtt gatgataagc cccatcacca cttcttccag
     6241 ctcttcagct tccgtttgcg tatcgggaat attatcgaga tccatcatac atcgttcctc
     6301 tcttttctta ccggcacgat tacccgtacc ggcatcgatt aaaatttcag ggcgttagcg
     6361 atatcttctt cgctctcttc tttatcaatc gcattttgtg ctttgttagc caccacaaca
     6421 aagggcagat aaattaacgt tgcgatacca aggttgaaga gtgcaaccag caatgcgacg
     6481 acgctaccgt tggtgttaaa gaaggctccc agaccggttg gcatggtcca cggtgcaata
     6541 ttggtcaccg gaggaataat gcccatgtag tacgctgcca gggtgattgc cgccagaatc
     6601 ggttgtacca gtacaaacgg gataaacatc accgggttca tgataattgg cagaccaaac
     6661 agaatcggtt cgttaatctg gaagatgccg gacggcagcg ccagttttgc cacctgacga
     6721 taatcagcac ggcgagaggc aataaagata gccaggatca ggcctaaagt cgcaccactg
     6781 cctccaagga aaataaagga gtccagcatc ggcttggccc agatatggaa ggtcttaccg
     6841 gctgccagcg ccgcttcgac ggaaccatat tgctgatagg tcgcgatatt ttccagcgcc
     6901 cacggcgtca taatgccgtt gtccagtgcg gtcagcgcca gcgcgccatg aataccgaag
     6961 aaccagagca gtggaacaaa gatcacatag gcccagccca ccacgctacc caacgatgcc
     7021 agtggggttg agatggtatc cataatgatc tgatggaagt tggtgcccca ggtattcaac
     7081 gcccaggcaa taatccccat cacggaaaga ataataaagc cgggaattaa tgccgagaag
     7141 gaacgcgata ctgaagcagg tacgctgtcg ggcagtttaa tgacccaatt gcggcggaca
     7201 ataaaggtaa acatttctgc caccaccagg ccaataataa tcccggagat gatattcgcc
     7261 ccacctaacc agtttgcgcc aaccgcatag gcctcaccga cactatatgg ggtgacggtc
     7321 ataaatgctg caacggataa caacccagcc gccagcgcat cgactttacg ctcttctgcc
     7381 agcgccatgc caataaagaa cggtgccatt aaagacatta tccctaatgt tccgttatat
     7441 acgttgccgc caataccttt cagaccatta agtgtttcaa tggttgaggc gtcgaggcga
     7501 atacctaagg aataaaaaaa cgacccctcc ccaaagctta gaaaaacgtt gttaattaat
     7561 acaaacatgg cccccgcaag ggttaacggc attaagcgaa taaagccatt tttgattgca
     7621 ttaacgtgtg gctgctttcc tattttaact gcaaaaggga ggagtacctt ttcaagcgat
     7681 gcaataacat tactcataga aaaataccct taaaaaccgc aatttaaata ttgcggtatt
     7741 gatttatgaa ataactcttt gacgggaaaa tttaaaataa attaatttgc tgcggctttt
     7801 ttaatcgctg caaccgcagc cttaagcacg cctaaaccat cgactttgcc ataaagcagc
     7861 gagtcaatta cttcaaccgg tttgttgggt aacaaacgct ggatttcggg caacatataa
     7921 gcaatctgcg gccctaataa cacgacatcg gcattctgac ctttttcacc agccagtgtt
     7981 tccggaaatg cttcaataat gaccggaact tcatattttt ctgcctgtgc gcgcattttt
     8041 gataccagta aagaggtaga catgcccgca gaacaaaaca gataaatgtg tttcttttcc
     8101 ataaaactgc cctcatcgac gattatctgt cagccagaca ctccgcaagc cttaacctgc
     8161 ttccatgctc tgggtaactt gcgaaaccaa catgactttt tttgtggctg aaaggagtat
     8221 acggtataga cctgagcaat agtatttctt tgaccttata aattgtctct ccttgacctg
     8281 cggttatgac cctcttcaca tttcgggcaa ataattcgcg aagataatta agaactcagg
     8341 ccaaaaaaaa ccggcgcaat ggccggtttc cgttgttact caagctttca gacgaagtga
     8401 ttacttcgca gcctgtggat cagtgtcgta ttcagcacag gtctgataac cggagttgat
     8461 gacatgtccg gtatcatcta acgcgacaaa gtaggtttct gctttaccat cacgttgacc
     8521 caggatgtag gtctggcaag taccacgagc atggatcatg ctcacttcag acgaaggttt
     8581 acccgcaatt tgtgcaacct gcgcccggct catgcctttt ttgacgtctt tcaccacagg
     8641 ctgtacaaac tggtctttgg tacgatcata agccgtacaa cccgccagca tggttaatac
     8701 cgccgctgca ctcagaattc ctgccatatt cttgttcata ttccgtccta ttgtttatca
     8761 gcgtgttaga taagcctgga atacattggg cgctttttca agcccatgaa cgaaacggct
     8821 ccgctttcag aggattcctg tatgacgttt taaccaccat tcagcccgct gtcgcttgtc
     8881 gtttcagtag caacgggtta gctttaagga agttttgtct tttctgtctg gaggggttca
     8941 atgacattgc aacaacaaat aataaaggcg ctgggcgcaa aaccgcagat taatgctgaa
     9001 gaggaaattc gtcgtagtgt cgattttctg aaaagctacc tgcaaactta tccgttcatt
     9061 aaatcactgg tgctcgggat cagcggcggt caggactcca cgcttgccgg taagctgtgc
     9121 cagatggcga ttaatgagct gcgccaggaa accggcaacg aatcactgca atttattgcc
     9181 gtacgcctgc cctatggtgt tcaggccgac gaacaagatt gccaggatgc cattgccttt
     9241 attcaaccgg atcgcgtatt aaccgttaat atcaagggcg cggtattggc tagcgagcag
     9301 gcattgcggg aagcatgcat tgaactgagc gattttgtcc gtggcaatga aaaagcgcgt
     9361 gagcggatga aagcacaata tagcattgcg ggtatgacca gcggtgtcgt ggtgggcacc
     9421 gatcatgcag cagaagccat taccggattc ttcactaaat atggtgacgg cggtacggac
     9481 attaatccac tgtatcgtct caacaaacgt cagggtaaac agttactggc agcattaggt
     9541 tgcccggaac acctttataa gaaagcgcca acggccgatc tggaagatga tcgcccttct
     9601 ctgccagatg aagtggcact cggcgtgacc tatgacaata tcgacgacta tctggaaggg
     9661 aaaaacgtac ctcaacaggt cgccagaaca atagagaact ggtatctgaa aaccgaacat
     9721 aaacgccgtc cgccaattac cgttttcgat gatttctgga aaaagtaata atttgcacct
     9781 attggattgt gcaaaaaaga gtaatttgtt cacgccggat gcggcgtgaa cgccttattc
     9841 gacctacaaa actatgcaaa ttcaatacat tgcaggagtc gaataggcct gacaggcgta
     9901 gcacgtcaga cggtgtaacc tttgtcatcg acccgcctct tttttaatcg cttcccggct
     9961 gttacactgg atagataacc agcattcgga gtcaacagtg gtacggcgtt taacttctcc
    10021 gcggctcgaa tttgaagctg cggcaattta tgaatatccc gaacatttac gttcattcct
    10081 taatgactta cccacccgac caggggtgta tctgtttcat ggcgaaagtg acaccatgcc
    10141 gctctatatc ggcaaaagcg ttaatatccg cagtcgggtg ctttctcatt tacgcacccc
    10201 ggatgaagcc gccatgctac ggcaatcccg gcgaattagc tggatctgca ccgcaggcga
    10261 aatcggcgct ctgctccttg aagcgcgatt aatcaaagaa caacagccgc tgtttaataa
    10321 acggttgcgc cgcaatcgcc agctctgtgc cctgcaatta aatgaaaagc gcgtcgatgt
    10381 ggtgtatgcc aaagaggtgg atttttcacg agcccccaac ctgtttggcc tgtttgccaa
    10441 taggcgcgca gctttgcaag cattgcagag catcgctgat gaacaaaaac tttgttatgg
    10501 tctgttggga ctggaaccgt taagtcgcgg tcgtgcgtgt tttcgttcag cgctaaaacg
    10561 ttgcgccgga gcatgctgcg gtaaagagag ccatgaggaa catgcgctac gcttgcgcca
    10621 gtctctggag cgtttgcggg tggtgtgttg gccttggcaa ggggcggtgg cgctgaaaga
    10681 acagcacccg gaaatgactc aatatcatat tattcaaaac tggctgtggc tgggggcggt
    10741 taattcgctg gaagaagcga caacgttaat tcggacaccc gccgggtttg atcacgacgg
    10801 ttataaaatt ctttgtaagc cgctgctttc cggtaactat gaaattactg aacttgatcc
    10861 ggcgaatgac cagcgagcca gttgatttca ctgaacaaca gtttaccttg cggttgtaaa
    10921 agccgcaggg tgtgcctgcc atcgaaccag cacgcgccct ggtcggtagt gagcagtttg
    10981 tcgcccagtt gccacgcccc gttaatcaca aacaccaccc cgccgcgtga gccaaaggta
    11041 gtaaacgtac gttcggcaat tctgactttc gccttacaga catccaggcg cgtcataata
    11101 ttgaaatcca tcgacatctg ccctgccgtc agtttcgctt ttaccacctg gtccgccgca
    11161 aaggcaaaag gctgtaacgg ttttaaggta tggttaaagt ggtctgcgct ttcaaggaac
    11221 atctcgccgc cttccagcaa cgtcactatc ctttccatac caggaaacaa agaaaactcc
    11281 ccattcgccg cgatggatgc aatgctggca cgccagtaaa aatcacgttt tgccggtggg
    11341 aaagtgcaaa tttcgcgcgt ttcaccggca gcgtttcgcc acaaattcac cgacatttta
    11401 cgcatatcaa agtattccat actcgctccc ggcccgttct tttcattgtt atcgttcagg
    11461 taaccgtgga tggttatcgg caactcgggt gatttacgtt agtggtgatc aggctttttt
    11521 tacaactgtt tgagaagaga aaagaaaacc gccgatcctg tccaccgcat tactgcaagg
    11581 tagtggacaa gaccggcggt cttaagtttt ttggctgaaa gattattcag cagttgcagg
    11641 cattttacct tttgccgctg gacgttctgt cagacgcttc tcaaaattag cattaaattg
    11701 ctttttctgt tccggcgtca ggatgttgta aattttgttc tgggtttcca tgtgcgccag
    11761 catgttagct ttgcgctgtt cttccatttt tgcgatctgc gcttcagctt ttgctttatc
    11821 gaaggtatcg ctggcaatga tgtcatgcat tgcgcgacgt tcttccagcg gcggacgttt
    11881 catctggtca cgctggcctt tcatgatttc gcgaatctgc tgtttctgcg cgtcggtcag
    11941 gttcaggtct ttgaacatca tgtcctgatg cggaccgaac ttgcctttgt ggtgcatcat
    12001 cggcttcgcg tcagccggtg ctgcggtagt ggtgtctgcg gcatgggcca ggttagccgc
    12061 gccaagagcc agggtagagg caacaaacag tgcagttaat ttacgcatat tctatatcct
    12121 tcctttcagt tatttattac ggctttctta agtagcgtgc cgtgttgacg agattaactt
    12181 tactgacttt agcgtcaatt aatcagagca acggtaaaac aatgaaagtg taaaaaacac
    12241 ttttgcgcca attatggaga aaaaaagaaa atttgatgga gagtgatgag agaatattac
    12301 aacaccatga ttttgcagag attatgaaga actataccgg atgactggtg ataaataaag
    12361 caaataacca ggattaatct gtattaattt ataagaaggt aacttaatac ccgcagaaag
    12421 atttctgcgg gtaagtatta gcttattttt tcgagcatta atcccgcgcg taatcccagc
    12481 gctaccaacg gattagggaa taacacatac tctacatcat gggttacggt aaaacgttcc
    12541 tcaccgtcct gcgccagcaa cgttcctttc ttaaacggca taaaattcag cgtgtcactt
    12601 gccatatgca tttcgaagga cggcgagtgg cgagtaattt gcgaaaccac ccgataacgg
    12661 agcggcggtg ttctcacgat accgacactc tcaccagata gcagcgcagc gattgcgctg
    12721 gcagttactg caaactggcg aagatcgttt tgcccaaagg gcaacgcttt gccaagttcc
    12781 agcgtacagg ccagcgcgcc aaaatgtctg gcgctgaaat gggtaaacgt accaccaggc
    12841 tcctgatgga acaccagcgc ctccagcccc gccgcaccca gccacgtcag gaatttctcg
    12901 tcccagggaa tgtcgcgttg cggtaataca ccgaactgcg gatgcaagga gccacgaatt
    12961 gcggtatgta gatcaaggtg ccagcgcaca gattctttgc cctggtcata aaaatcttcc
    13021 aggcactgtt ccagttcgcg cgcccgacag gtttccccgc tttcagcaaa cagctgccaa
    13081 cgaccgccaa acattcggtt catatcgcta tggcaataac gtttcccttg cttcagcgca
    13141 ggaggattcc cgaggatcac cagcaaccgc caccgtaacg ggatctcgcc gtgagatatc
    13201 gcgccaagca atgcatcaag catctccaca ggtgccgtct cattaccgtg gatccccgca
    13261 gaaatcacca gagcgccttg cggtggcgtt aatggcgtca gttccagcac accatcgccc
    13321 agccagcgcc agcgaacgcc gttgatttct cgctcggtga tgacaggttt tttactcgtt
    13381 aaggtcagag caagaaaatt atccattgcc ccctccctcg cgctggaacg gataaaccga
    13441 accgagattc agtaattggc tcaatacatc cagtgcttcc cgcccttcgc gcagcaattg
    13501 cgggtcggcc agatcggcag cagtaagacg atcgcggtag taacgatcca cccagtcatt
    13561 gagcgcatta aacaaggtat cgttcatcat caccgccgga ttcaccgccc ggcgttcttc
    13621 ttgtgtcaat accacccgca accgcaggca cgccgggcca ccgccattcg ccatgctttc
    13681 acgtaaatca aagactttta gttcgctaat cgggttgtca gcggcaagga gttcattgag
    13741 ataaccccat actccggcgt gttcccgaca ctcctgaggc agcaccaaca tcatggaacc
    13801 atcatcgcgg ctcaacagtt ggctgttaaa cagatatgta gacaccgcat ccgacacgga
    13861 aacctgagtt gccggaactt ctatcgccat aaaaccgttc acccgcgcac gcaggtttgc
    13921 cagtaactgt gactggcgag cgaacgcctg ttggtggcaa aacagcacct ggcggttact
    13981 cacggcaatc acgtcattat gaaaaacgcc ctggtcgata acgtccgggt tttgctgggc
    14041 gaaaatcacc tgttggggat tcacctgatt cagccttgcc accgcctcgc tggcttcgcg
    14101 agtctgtcgc gccggataac gggaaggccg ggtatcattg ccctcttctc gcccgtagac
    14161 aaaaagttgc atacccggtt caccgtaatg accgccgaga cgattgtggt ttgccgcccc
    14221 ctcatcaccg agcaaggcta cctgcggcaa cgccgaatgg acactaaatt tctcttcgtc
    14281 gttaaaaatc gcttttaaca gcgattcagt gacgggcgct tccagcgaac ggtgaaattt
    14341 attgttcagg ttagcaacgg tgagatgcac tttgccatcc agcgtatcgg cagatggcgc
    14401 gatcgttgcc gcattggcta cccacattgg cgaagcggaa ctgacgctgg aaagccagtg
    14461 cggtgcctgg cgtacaacct tttccagtac ctgctcatcg ctaccactga atcccaactg
    14521 acgcagcacc ggaataaacg gacgctcgtg aggcgggatc acggcctggg ggaatcccgc
    14581 atcggcaagg gttttcattt tcagtaagcc ctgttttgcc gccagtcgcg ggttagagac
    14641 ctgaaaacgg tgacgggtag aggctttatt accaaacgac aggcccgcgt aatgatgcgt
    14701 cagccctacc agcccgtcga aattaacttc ccaggcgttc atcgcaccac ctcatcggaa
    14761 aaatccagcc cggggttaag cgttgcgggc aaagttaacg agtccgactc caggctcgcc
    14821 ataggccatg cgcagtaatc tgcggcatac caggcgctgg ggcgatggtt accggatgca
    14881 ccaatgccgc cgaatggcgc ggtactggca gcaccagtaa gcggtttgtt ccagttaaca
    14941 atccccgccc gcgcctccag caacagttga tcgaattttt cccgctcggg ggaaaccaga
    15001 ccgcaagaga gtccgaagcg agtgttattc gccattcgaa tcgcttcctc gaaagaatca
    15061 taacgccaga cgcgcaataa cggtccgaac acctcttcat ctggtacgcc agcaacgcct
    15121 gtcatttcaa tgatccccgg cgtcagcaac gatgtccctg cttgtaataa gcgcggcgca
    15181 agcagggttc gtccacccat cgcttccagt tgctgccagg cagtaaacac ctgctgtgcg
    15241 gcctgttcag aaatcagccc gccaataaac ggctgcggtt cgtcatccca gttgcccggc
    15301 gttaatcgct ggctgacggc aaccagacga gcaagaaatg catcgccctg cgccccgctt
    15361 ttcagcaata cacggcgggc gcaggtgcag cgttgcccgg ctgtaacaaa cgccgactga
    15421 atggtcagat ggacagccgc gtcgatatcc gccacctcat cgataatcag cgggttattg
    15481 ccgcccatct caagggcgag aattttctcc ggctgaccgg agagctggcg atgcagctgg
    15541 tagccggtat tggcgctgcc ggtaaacagc aagccgtcga gatcctccag cgcactcagc
    15601 gcctgacccg tttcacaccc gccctgcacc aggttcagca cgcctggcgg aaagccagcc
    15661 tgctgccata aacgcattac cgcttcgcca ctccacggtg tcagttcgct gggtttaaag
    15721 ataatggtgt tacctgccag caatgcggga acgatatgtc cgttcggcaa atgaccaggg
    15781 aaattatacg gcccaaacac cgccagcacg ccgtgcgggc gatgtcgcag gctcgccgca
    15841 ccgtccggca tttcactacg ctgctcgccg gtacgaacgt gatacgcctt aattgatatc
    15901 gcgattttat tgatcatcgc cgtcaattcg gttgccgcct cccagcgcgg cttgccggtt
    15961 tctcgggcaa tgattgcggt taattcgcct ttattccttt ccagcaatcc ggcaaagcgt
    16021 tcgacaacgg cctgacgttc agcaaatgag agccgcgccc agcgcggaaa cgccgcacgg
    16081 gctgcccgac aagcctgccc gacctgagcg gcatcggcat cattgccttg ccataacacc
    16141 tcgcccgata ccggattacg cttcacacgc gatgcaccct ggcccgttat ccagtcaccg
    16201 ttaatccata aagtcatgct gttttctcct ctgcgcacag gcgcaccaga cgaacgcgat
    16261 cccctgcatg gcatttgagg gcatccagtt gtgcggcggt taaaatcaaa cgctcggttg
    16321 ccggatcggt acgcaccagc accacgcgga aatggtgata attttcattg gcgaccaggc
    16381 aggccggaaa atcgccctgc gcaggctgcc cttctgccac ttccaccagc cgacttttac
    16441 ggatggcgcg cacgcggtcg atgtcacact caagcgtcgg cccaccgtcg aagatgtcga
    16501 tatagttgcg gtagcgaaaa ccttctttct ccagcaccgc gcgggcaggc gcggtttgcg
    16561 gatgtacctg accgatgacg tcctgggctt cctgggataa aaagtgcgta tagatcggat
    16621 gtttcggcat cagttctgca ataaatgcct tttgcccggt gccgcagaga aaatcggcgc
    16681 ggctaaaatc catcgaaaag aagcgtttac cgaggctttg ccagaacggt gaatagccgt
    16741 gttcgtcaat caccccgcgc atttcagcaa ccactttgtc attaaacttg tcgcgaaaag
    16801 ccgccataaa cataaagcgc gatttcgaca gcaaatagcc gttgccctct ttgcgccagt
    16861 ccgggtcgag aaacagcgtg cacagctcgc tgctgccggt gtgatcgtta ctgagaaaca
    16921 acgtcggcaa tgcgttatag acattcagct cttttgaggc gtgaaccaac gtgccgacgc
    16981 gatagttgta ccagggatcg ttcagcccaa ctgccacctc aatggcgcaa atccccgcca
    17041 cggtgcctgt ctcgctatct tccagtacaa acacataacc ctgctcactt ttgggcagtt
    17101 caccttgcca ggttttgatt gccctttcga tacgcgccga aagcgtggct tcattggcgg
    17161 gaagcgacgt caggccgccg cccgttttgc tggcaagctg catcagcgcc gagacatctg
    17221 agcgctcaac gggacggatg accatcatga tgaacctcgg ctaacaaagt gttcgcaagc
    17281 aactgcaaag cgatccagtc cggtcgtcac ctcttcttcg ctgacattga gcgcaggcgc
    17341 aaaacgcacc acgttgccac ccgcaatcag taccatcacg cctgctttcg ccgcttcctg
    17401 agagatctgt ttcgcttgcc cggcgtaatc ggcattcagt acacagccaa tcagcaaacc
    17461 taagccgcga acttcattga acaaaccata gcggtgatta ataatattaa gacgctcaac
    17521 aaaccagtcg tgacgttgct taaccccgtt aagcatctct ggcgtattga tgagctccag
    17581 cactttgcct gccaccgccg aggccagcgg attgccgcca taggtggtgc catgagtgcc
    17641 aacggtcatc acgctggcgc actcttcggt tgctaacaac gcaccgaccg ggaaaccgcc
    17701 gcccagagct ttggcggtag ttaacagatc aggcgtcacg ccgtagtgca tataggcata
    17761 cagttccccg gtacgcccga cgccggtttg tacttcatca aaaatcaaca gcgcattgtg
    17821 gcgatcacac aattcacgca gaccttgtaa aaatgcgttg ctggctggca ccacaccgcc
    17881 ttcaccctgg atgggttcga caatcaccgc acaggtagag tcgtcaatca gcgcgctggc
    17941 agagttaata tcgttatatg cagcatgacg gatatctggt ggcagtggcg caaaatcctg
    18001 tgaataggct ggctgcccac ccgcactaac agtaaacagc gtgcgaccat gaaacgcatt
    18061 tttaaacgcc acgataccgc tcttatggct gccgtagcgg tcgtgagcga atttacgcgc
    18121 cagttttagc gccgcttcgt tggcttccgc accggagtta caaaagaaaa cgcgatcggc
    18181 aaacgtggcg tcgatcaatt ttttcgccag tcgcagtacc gactcgttgg tgtaaccgtt
    18241 gccggtatgc cagaacttac tcgcctgttc gttcagcgcc tcacgcagtt ccggatgcgc
    18301 atggcccagc gcgttcaccg caatgccacc cgcgaagtcg atatactctt tcccctgctg
    18361 atcccacaag cgcgaacctt cgccacgtac cggtataaac ggtgccggag cgtaaacagg
    18421 tatcatccat tcatcaaagt tttcacgcgt aattggctga gacatagcga cctctacagt
    18481 aaatagctcg ttatttatat gttaataata agtaatgttt tcgctgtaaa tgtagattgc
    18541 agggttcgtg ccagccagtg atgaaagtgc ataaacggcg gaggctaact ggaaatcaag
    18601 gagttataac caaaccatat gcatttaaag tgcatattaa gtgaatacgt ttgcgatgtg
    18661 ggtgaataaa aagaataaaa aacgcaatgt tatgcagaag taaaatataa ttctggaatt
    18721 gtgatcattg acgaaattta ctggaaatta ctgcgccatt ctgacgcagc gcgcaccaaa
    18781 agcgggcatt ttttgcgcca tcgttgacat cattaacaac catcgatcaa atcacttaac
    18841 aacaggcggt aagcaaagcg aaattctgct accatccacg cactctttat ctgaataaat
    18901 ggcagcgact atgaaatttg tctcttttaa tatcaatggc ctgcgcgcca gaccccacca
    18961 gcttgaagcc atcgtcgaaa agcaccaacc ggatgtgatt ggcctgcagg agacaaaagt
    19021 tcatgacgat atgtttcccc tcgaagaggt ggcgaagctc ggctacaacg tgttttatca
    19081 cgggcagaaa ggccattatg gcgtggcgct gctgaccaaa gagacgccga ttgccgtgcg
    19141 tcgcggcttt cccggtgacg aagaagaggc gcagcggcgg attattatgg cggaaatccc
    19201 ctcgccgctg ggcaatgtca ccgtgatcaa cggttacttc ccgcagggtg aaagccgcga
    19261 ccatccgata aaattcccgg caaaggcgca gttttatcag aatctgcaaa actacctgga
    19321 aaccgaactc aagcgtgaaa atccggtgct gattatgggc gatatgaata tcagtcctgg
    19381 cgacctggat attggcattg gcgaagagaa ccgtaagcgc tggctgcgta ccggaaaatg
    19441 ttctttcctt ccggaggaac gcgaatggat ggagagactg atgagctggg gattggttga
    19501 taccttccgc catgcgaatc cgcaaacagc agatcgtttc tcatggtttg attaccgctc
    19561 aaaaggtttt gacgataacc gtggtctgcg catcgacctg ctgctcgcca gccaaccgct
    19621 ggcagaatgt tgcgtagaaa ccggcatcga ctatgaaatc cgcagcatgg aaaaaccgtc
    19681 cgatcacgcc cccgtctggg cgaccttccg ccgctaattt agcagctctc ctggctcaaa
    19741 ctgggtcagg agaattaacc ttgagaaaaa tcaacaaact gtcagtaatg atttgttgcc
    19801 tgccgtcctt tgttataccg tcactgcgtt tttagttgtc tgaccacttc tctattatca
    19861 agtttgatat aggaaactcc acgatgaacg ctgagcgtaa atttcttttt gcctgtctta
    19921 tttttgcgct ggccatttac gctatccacg ctttcggttt attcgatctg ctcaccgatt
    19981 taccccactt acagacactc attcgccaga gcggattgtt cggttatagc ctctatattc
    20041 tgttattcat cattgccacc ctctttctgt taccaggaag catattggtg atcgcgggag
    20101 gaatagtttt tggcccgttc ttagggacac tactctcatt aattgccgcc acgctggcct
    20161 cctcgtgctc attcctgctg gcgcgctgga tgggacgtga tttactgctg aaatacgttg
    20221 gccatagcca taccttccag gccattgaaa aaggcattgc gcgtaacggt attgattttc
    20281 ttattctgac ccgcttaatc ccgttgtttc cttacaatat tcaaaattac gcttacggat
    20341 taaccacaat cgccttctgg ccttataccc ttatttcggc actcacgacc ctgcccggta
    20401 ttgttattta taccgtgatg gcaagcgatc tcgccaatga aggcattacg ctgcgcttta
    20461 ttttacaact ctgtctggcg ggcctggcgc tgtttattct cgtccagctc gcaaaactct
    20521 acgcccgaca caaacatgtg gatctgtctg cttcgcgccg caacccactt actcacccaa
    20581 aaaatgaagg atagaacgat gttgcaacat tattcagtgt catggaaaaa aggactgact
    20641 gcactctgct tactggctgt tgctgggctt agcggctgcg atcaaaaaga gaatgcggcg
    20701 gcaaaagtgg aatacgacgg actttcgaac agccaaccgc tgcgcgtcga tgccaataac
    20761 catacggtaa ccatgctggt gcaaattaat ggtcgtttcc tcaccgacga cactcgtcac
    20821 ggtattgtgt ttaaagatgg ctccaacgga cataaatcgc tgtttatggg ttatgcgacc
    20881 ccgaaagcat tttatgaagc cctgaaagag gcaggtggta caccgggcga aaacatgacg
    20941 atggataata aagaaacgac tcatgtcaca ggcagcaaac tggatatttc ggtcaactgg
    21001 caaggggcgg caaaaacgta ttccttcgat gaagtgattg ttgacagtaa tggcaagaaa
    21061 ctggacatgc gctttggcgg taatttaacg gcagcagaag agaagaaaac gggttgcctg
    21121 gtgtgtctgg atagctgccc ggtcggcatc gtcagcaatg caacatacac ttatggtgcg
    21181 gttgaaaaac gaggtgaagt taaattcaaa ggcaatgcct cggttctccc ggcgaataac
    21241 acgctggcaa cggttacctt taaaatcacc gaataaagcc aggataaagg atgatgaaga
    21301 tgcaatcgcg taaaatctgg tactaccgta taaccctcat catcctgttg ttcgctatgc
    21361 tgctggcatg ggcgctgctt cccggcgtcc atgagtttat caatcgcagc gttgcggcgt
    21421 ttgccgccgt ggaccaacag ggtatagaac gctttattca gtcttacggc gcactggcgg
    21481 cagttgtctc gttcttgttg atgattttac aggccattgc tgcaccgcta cctgcgtttt
    21541 tgatcacctt tgccaatgcg tcgctgtttg gcgcgttctg gggcggctcg ctgtcgtgga
    21601 ccagttcgat ggccggcgcg gcactgtgct tttttatcgc cagagtgatg ggccgcgaag
    21661 tggtggaaaa attaaccggc aaaaccgtac ttgacagtat ggacggcttt ttcactcgct
    21721 acggcaaaca caccatcctg gtctgtcggt tattgccttt tgtccctttc gatccaatca
    21781 gctatgctgc cggtttgact tcaatacgtt ttcgctcgtt ttttatcgcc accgggcttg
    21841 gtcagttacc ggcgactatt gtttattcct gggcgggcag catgttaaca ggcggtacct
    21901 tctggtttgt caccggactg tttattctgt ttgccctgac cgtggtgatt tttatggcga
    21961 agaaaatatg gcttgagcgc cagaagagga atgcctgatg ggtttaccgc cgcttagcaa
    22021 aattcctttc attttacgtc cgcaggcgtg gctgcatcgt cgccattacg gcgaggtgct
    22081 aagccccatt cgctggtggg ggcggatccc gtttatcttt tatctggtgt cgatgtttgt
    22141 tggctggctg gagcgcaaac gctcaccgct cgatccggta gtacgatcgc ttgtcagcgc
    22201 gcgcattgcg caaatgtgcc tgtgtgagtt ttgtgtggat atcaccagta tgaaagtcgc
    22261 cgagcgcacc ggcagcagcg ataaactgct ggcagtggct gactggcggc aaagcccgct
    22321 ctttagcgat gaagaacggc tggcgctgga gtacgccgaa gccgcaagcg taacgccgcc
    22381 aacggtcgat gatgccctgc gtacccgact ggctgcgcat tttgacgctc aggagctcac
    22441 cgaactgacg gcattgatcg gcctgcaaaa tctgtcagcc cgttttaatt ctgccatgga
    22501 cattcccgct caggggctgt gccgtattcc ggaaaaacgt tcttaaggag agatgatgcg
    22561 ccattgtggg tggttgctgg gattgttatc gctgttttct ctggcaacac atgccagtga
    22621 ctggcaagaa attaaaaatg aggccaaagg gcaaactgtc tggtttaacg cctggggcgg
    22681 cgataccgca attaaccgct atctcgactg ggttagcggc gagatgaaaa cccattacgc
    22741 tataaacctg aagattgttc gtctggcgga tgccgcagac gcggtgaagc gcattcagac
    22801 cgaagctgct gccggacgta aaacgggcgg ctcggtggat ctgctctggg tgaacggcga
    22861 aaacttccgc accttaaaag aggccaattt actgcaaacc gactgggcag agactctgcc
    22921 caactggcgc tatgtcgaca cacagctgcc ggtgcgggaa gatttttcag tgcctacaga
    22981 aggggctgaa tcgccctggg ggggcgcaca actgacattt atcgcccgcc gcgatgttac
    23041 gccacagcca ccacaaacgc cgcaagcctt actggagttt gctaaagcca atcccggcac
    23101 ggttacctat ccgcgcccac cggactttac cggcacggcg tttcttgaac agttgctgat
    23161 tatgctgacg cccgatcccg ccgcattaaa tgaagcgccg aacgatgcga ctttcgcccg
    23221 tgtcactgct cccttgtggc aatatcttga tgcgctgcat ccgtatttgt ggcgcgaagg
    23281 aaaggatttc ccgccttcgc ccgcgcggat ggatgctctg ctgaaagccg gaacatttcg
    23341 cctgtcgctg acctttaacc ccgcgcatgc gcagcaaaaa atcgccagcg gcgatttgcc
    23401 tgcaagcagt tacagttttg gctttcgcga ggggatgatt ggcaacgtgc atttcgtcac
    23461 cattcctgcc aacgcgaatg ccagtgctgc ggcgaaggta gttgccaatt tcttgctctc
    23521 acccgatgcg caattgcgta aagcagatcc cgctgtctgg ggcgatcctt ctgttctcga
    23581 tccgcaaaaa ctgcctgatg ggcagcgcga aacattgcaa tcaagaatgc cgcaagatct
    23641 gccgccggta ctggctgaac cgcacgcagg ttgggtaaat gcgctggaac aagaatggct
    23701 acaccgttac ggtacgcatt aatctttttg ctgtgggcga tggtggcggt gatttatgca
    23761 ccgctgatcc cggcagctct cacgctgata tcgcctgctt tgtcgttgac acactggcag
    23821 gcgttatttg ccgatccaca gttaccgcag gcattactgg caacgctggt gtcgacaacc
    23881 atcgcggcgg tcggggcgtt gttgattgcc ctactggtga ttgtggcgct gtggcctggg
    23941 ccgaaatggc agcgtatgtg cacccgtctg ccgtggctgc tcgccattcc ccatgtggct
    24001 tttgccacca gcgcccttct gctctttgct gacggagggc tgctttatga ctatttcccg
    24061 tatttcactc cgccaatgga caaattgggt attgggctgg gcttcacgct ggcggtgaaa
    24121 gaaagcgcat ttctgctgtg gatcttagca gcggtattga gtgaaaaacg gcttttacag
    24181 caagtcatcg tgctggattc gctgggctac agccgctggc aatgcctgaa ctggctgctg
    24241 ttgccctccg tcgcgcctgc gctggcaatg gcgatgctgg cgattgttgc ctggtcgctg
    24301 tcggtcgtag atgtggcgat tattcttggg ccaggtaatc caccgacgct ggcagtaatt
    24361 agctggcagt ggttaactca gggcgacgcc gatcaacaaa cgaaaggcgc actcgccagc
    24421 ctgctgctga tgttgttact cgccgcctac gttttgctgg gctatctgct atggtgtacc
    24481 tggcggcgta ctattccccg cgtagatggc gttcgtaagc ctgccacgcc tttattgcca
    24541 ggcattacgc tggcgagttt tttacccttg accagtgtgc tgtgtgtggt tctgctggcg
    24601 atcctcgcgg atcagtcgac gatcaatagt gaagcgctca tcaacagcct gacaatgggg
    24661 ctggtggcga cattcatcgc tttgctcctg ttactgctgt ggctggaatg ggggccacag
    24721 cgtcgccagt tgtggctatg gttgccgatt ttattaccgg cactgccgct ggtggcgggc
    24781 cagtacacgc tggcgctatg gctgaacatg gatggaagct ggacggcggt ggtctggggg
    24841 catctgctgt gggtaatgcc gtggatgctg tttatcctgc aacccgcctg gcagcgcatt
    24901 gattcacggt taattttgat tgcacaaaca ctgggctggt cgcgggccaa aatcttcttt
    24961 tacgtgaaat gtccgcttat gctacgccct acgctgattg ccttcgcggt gggattttca
    25021 gtcagtattg cgcagtatat gccgacgctg tggctgggcg cggggcgttt tccgacgctc
    25081 accactgagg cggtggcatt aagcagcggc ggcagcaacg gtattctcgc cgccctggct
    25141 ttatggcaac tgctattacc gcttattatt tttgccctga ccgcgttagt cgcaaaatgg
    25201 gtaggttatg tcagacaagg actccgctaa tgctctgcgt gaaaaatgtt tcgctacgtt
    25261 taccagaaag ttgcttgctg acaaacgtta actttacggt ggataaaggt gacattgtca
    25321 cgttaatggg gccgtctggc tgtggaaaat ccactctgtt ttcatggatg attggtgcac
    25381 tggccggaca gttttcttgt acaggtgagc tatggctcaa tgagcaacgg attgacatgc
    25441 tacccaccgc acagcgtcag attggcattc tttttcagga tgcactgtta tttgaccagt
    25501 tcagtgtcgg gcaaaattta ctgctggcgc taccggcgac acttaagggg aatgcccgac
    25561 gtaatgccgt gaatgatgca cttgagcgtt caggccttga cggagctttc catcaggatc
    25621 ctgctacttt gtctggcggt cagcgagcgc gcgttgctct gcttcgcgcc cttctcgctc
    25681 agccaaaagc gttgctactg gatgagccat tcagccgtct tgatgtggct ctgcgcgata
    25741 attttcgcca gtgggtgttc agcgaagttc gcgaactggc gatccccgtc gttcaggtaa
    25801 cgcacgatct ccaggatgtt cctgctgata gttctgttct ggatatggcg cagtggtcag
    25861 aaaattacaa caaactgcga taacgcaaag tttttctcaa tgcgtcagtt cagaatggcg
    25921 cactcaaaac tacaatgtcg ggattttcga tgaaacgtgt ttctcaaatg accgcgctgg
    25981 caatggcttt agggctggct tgcgcttctt cgtgggccgc tgaactggcg aagcctctta
    26041 cacttgacca gcttcaacaa caaaatggca aagcgataga tactcgctcc agcgcgtttt
    26101 ataacggctg gccacaaacc ttaaatggcc cttctggtca tgaacctgcc gccttaaacc
    26161 tctctgctag ctggcttgac aaaatgagca ccgaacagct caacgcgtgg atcaagcaac
    26221 ataacctgaa agccgatgcc ccggtggcgc tgtacggtaa tggcaaagat gtcgacgccg
    26281 tcaaaacgcg actgcaaaaa gcaggtttta cgcatatctc cattctgagt gacgcgctaa
    26341 gcgaaccttc ccgtctacaa aaactgccgc actttgagca gctggtttat ccgcaatggc
    26401 tgcacgacct gcaacaaggt aaagaggtta cggcgaaacc tgccggtgac tggaaagtca
    26461 ttgaagcggc caggggcgct cctaagcttt accttatcag ccatattccc ggtgctgact
    26521 acatcgatac caacgaagtg gaaagcgaac cgctgtggaa caaagtttct gatgaacaac
    26581 taaaagcgat gctggcaaaa cacggcattc gccatgacac cacggtcatt ctgtacgggc
    26641 gtgacgtata cgctgcagcg cgtgtggcgc agattatgct ttatgccggc gtgaaagatg
    26701 tgcgcctgct ggatggcggc tggcaaacct ggtccgacgc gggtctgccc gttgagcgcg
    26761 gtacgccacc gaaagtgaaa gcggaaccgg atttcggcgt gaagatcccg gcacaaccgc
    26821 agttgatgct cgatatggaa caggcccgtg gactgctgca tcgccaggat gcatcgctgg
    26881 tgagcattcg ttcgtggcca gaatttatcg gtacgaccag cggttacagc tatattaaac
    26941 caaaaggtga aatagccgga gcacgttggg gacacgctgg tagcgactcg acgcatatgg
    27001 aagatttcca taatccggat ggcaccatgc gtagcgccga tgatattacc gctatgtgga
    27061 aagcatggaa tatcaaacca gatcagcaag tttcattcta ctgcggcacc ggctggcgcg
    27121 cgtccgaaac ctttatgtac gcacgagcaa tgggctggaa taacgtctcc gtttacgacg
    27181 gcggctggta cgaatggagc agcgatccaa aaaatccggt agcaaccggt gaacgcggcc
    27241 cggacagcag caaataacat tgcaattact gacgctggag cgacttcagc gtcagatacc
    27301 cactccacac ccgcgtaaag gtcgtcatcc agcacaacgc accgaagatc cacgcaaacc
    27361 acgcaaacca tgcagggaat aaacatccca gcacaaacag taagatcgtt tctgtgcctt
    27421 cggttaatcc ccccagataa taaaacgatt tatgcgcata accgggatta tcaatctgat
    27481 atttcgccgc cagcgcagca aaagcgagaa aactgctacc cgtgccgata aacgcaaaca
    27541 gcagccagcc gcctgccagg gcgttttgct gcggtgcggc gaggataaac ccaaaaggta
    27601 ccagcgcgta aaaaagaaaa tcgagagaaa tatcaagaaa gccgcccgcg tcggtaagcc
    27661 ctctgcgccg cgccagcgca ccgtccagac catcaagcag cctgttcaac aaaatgacga
    27721 caagcgccgc cagataccag cccagcgcca gaaaaggcag tgccagcaca ccgatggcaa
    27781 atcccactaa tgttaaacca tctggcgtaa tgcccggttt atcaagaacc cgcacacact
    27841 gatgcaacaa cggtttaatc cggggatgaa gatggcggtc tagcacaggc actccttaaa
    27901 tataaagcct ttctgattga gcaacagtgc ggatattatg gcatttttcg cttatctgcc
    27961 cgtgtgtaat ttatgaaaat gattgaagtt gttgccgcca tcattgaacg tgatggcaaa
    28021 attttactcg cgcaacgccc cgcccagagc gatcaggcgg gattatggga gtttgccggt
    28081 ggtaaagtcg agccggatga aagccagcag caggcgctgg tgcgtgagtt aaacgaagaa
    28141 ttgggcatcg aagcaactgt gggtgagtat gttgccagcc atcagcgaga agtttcgggc
    28201 cggattatcc atcttcatgc ctggcacgta cccgacttcc acgggacgtt acaggcacat
    28261 gaacatcagg cgctggtctg gtgctcacct gaagaggcgc tgcaatatcc gctggctcct
    28321 gctgacattc cattattaga ggcgtttatg gctttacgcg ccgccagacc agcggattag
    28381 tgctaagggt tttgtcatcc cgctggcatt gcagcagtat tccttcggct ttaattaccg
    28441 ccccttcaga ataattttta tcctgataaa cgcagcactg agtacagggc tgcgctgact
    28501 gcccgcctga actgaccact tctggcggta cgtttacctc cacgtccgga ccataatgcg
    28561 ggttagccag tgcaattaat ggaaatgcta atactacggc gaacaatgct cgactcacag
    28621 ggaactcctt aacgttattg tctctgctac tgataacggt agccgggtgg caaaacttta
    28681 gcgtctgagt tatcgcattt ggttatgaga ttactctcgt tattaatttg ctttcctggg
    28741 tcattttttt cttgcttacc gtcacattct tgatggtata gtcgaaaact gcaaaagcac
    28801 atgacataaa caacataagc acaatcgtat taatatataa gggttttata tctatggatc
    28861 agacatattc tctggagtca ttcctcaacc atgtccaaaa gcgcgacccg aatcaaaccg
    28921 agttcgcgca agccgttcgt gaagtaatga ccacactctg gccttttctt gaacaaaatc
    28981 caaaatatcg ccagatgtca ttactggagc gtctggttga accggagcgc gtgatccagt
    29041 ttcgcgtggt atgggttgat gatcgcaatc aggtacaggt caaccgtgca tggcgtgtgc
    29101 agttcagctc tgccatcggc ccgtacaaag gcggtatgcg cttccatccg tcagttaacc
    29161 tttccattct taaattcctc ggcttcgaac aaaccttcaa aaatgccctg actactctgc
    29221 cgatgggcgg tggtaaaggc ggcagcgatt tcgacccgaa agggaaaagc gaaggcgaag
    29281 tgatgcgttt ctgccaggcg ctgatgactg aactgtatcg ccacctgggc gcggataccg
    29341 acgttccggc aggtgatatc ggggttggtg gtcgtgaagt cggctttatg gcggggatga
    29401 tgaaaaagct ctccaacaat accgcctgcg tcttcaccgg taagggcctt tcatttggcg
    29461 gcagtcttat tcgcccggaa gctaccggct acggtctcgt ttatttcaca gaagcaatgc
    29521 taaaacgcca cggtatgggt tttgaaggca tgcgcgtttc cgtttctggc tccggcaacg
    29581 tcgcccagta cgctatcgaa aaagcgatgg aatttggtgc tcgtgtgatc actgcgtcag
    29641 actccagcgg cactgtagtt gatgaaagcg gattcacgaa agagaaactg gcacgtctta
    29701 tcgaaatcaa atccagccgc gatggtcgag tggcagatta cgccaaagaa tttggtctgg
    29761 tctatctcga aggccaacag ccgtggtctg taccggttga tatcgccctg ccttgcgcta
    29821 cccagaacga actggatgtt gacgccgcgc atcagcttat cgctaatggc gttaaagccg
    29881 tcgccgaagg ggcaaatatg ccgaccacca tcgaagcgac tgaactgttc cagcaggcag
    29941 gcgtactatt tgcaccgggt aaagcggcta atgctggtgg cgtcgctaca tcgggcctgg
    30001 aaatggcaca aaacgctgcg cgcctgggct ggaaagccga gaaagttgac gcacgtttgc
    30061 atcacatcat gctggatatc caccatgcct gtgttgagca tggtggtgaa ggtgagcaaa
    30121 ccaactacgt gcagggcgcg aacattgccg gttttgtgaa ggttgccgat gcgatgctgg
    30181 cgcagggtgt gatttaagtt gtaaatgcct gatggcgcta cgcttatcag gcctacaaat
    30241 gggcacaatt cattgcagtt acgctctaat gtaggccggg caagcgcagc gcccccggca
    30301 aaatttcagg cgtttatgag tatttaacgg atgatgctcc ccagggaaca tttcttatgg
    30361 gccaacggca tttcttactg tagtgctccc aaaactgctt gtcgtaacga taacaagctt
    30421 caagttcagc atccgttaac tttctgcgat agcagcagat atgccagtaa agaaatccca
    30481 ttagactatt tttttgataa tcttcttcgc tttcgaacaa ctcgtgcgcc tttcgagaag
    30541 caagcattat ataatgccag gccagttctt cttcaattgt cccgttttga aaagctgtgc
    30601 ttgatatcga gatcatccat gataattccg ccgcccatat tagcttcgcc gaggatttac
    30661 cggagctatg attagcgcaa tcagagatat agtctgaggg aaaaacagca aatttattca
    30721 acaaggcgat aacctgctct ggggcttcct ccatgtttgc tttaaaggta ttggctccat
    30781 ggtcgccaga aagaaaatgc tccattaagg cacaataact ttcgctatct tcgatacccc
    30841 attgttcctc taaagactcg cgtcttttac ttatgatatc gatcgagtca aaaggaagca
    30901 catgatattg gaaggtatct ttgccaggtt caggctttcg cggccagaac tccagcgttt
    30961 cagaccattg cttatgatag aatcgataag gtgcgatcaa ttgtagcgcc tgtaatttct
    31021 cgatactgag cggctcaata cctttagcct gataataatg cagctgttct ttttttgctt
    31081 taaaaccggc ccgaacaata agccccatca taattaatag ataaagaaaa gagcatcccg
    31141 cggtaatcag gcctctttca ttcaaaccgt tggatgttat cgctgcgaat acaaacatta
    31201 cagcgacaac acatgttaaa taaaaccccc acttacaaag cagcatggtc ttattttctt
    31261 taatcatccg ttcaaaatta ctattaaata tttcccagcc attaaaagaa tacttctcgc
    31321 tcccaggatg gttttgtaat aaaacttttt tcatcacatt ccctgttatt acatacttta
    31381 gaggcgataa ttttatccat gcaaaaaaaa tatccaacga attttcttga tccgctggat
    31441 atttcagaat atgactcgaa tagcacgaaa gattcgctcg cttacgctat cgccccgctt
    31501 ccggcttcat ctgctggcgg actttttttc gcactacgtt tacgcggtgc agcctttttc
    31561 ttatcagcac tgccaccact gcccggagcc acaatgccgc gaaactgccg caccggagta
    31621 cgtttggctt gatcaataag ctgatatagc gtccccacca gcggctgcat aaagtcctga
    31681 tagcgacact gcttttcgct gatttgcgtc agcaccgatt cccagtgcgc ggtcatgtcc
    31741 ggtcgcgtag ccatttctgg cagcgaatgg aataacgctt ttccggcgtc ggtggagtgg
    31801 atatagcgcc cttttttgat gaggaaacca cgcttgaaca acagttcaat aatcccggca
    31861 cgcgttgctt ctgtccccag accatcggtc gcacgaagga tctttttcag atctttgtcc
    31921 tgcacaaagc gcgcgatccc ggtcatcgcc gaaagcagtg ttgcatcggt aaaatggcgc
    31981 ggcggctggg tttgccgctc taccacttca cctttttcac acagcaactc atcgcctttc
    32041 gccaccacag gcaatggcgt accgtcgttt tcttcatcgc gctctttgct gcctaacagc
    32101 gtgcgccagc ctgcttcagc aagaaaacgc gctttagcga caaatttgcc tttggcaatg
    32161 tccagttcga taacacactt gcggaacacc gcatccgggc agaactgcat cagatactga
    32221 cgggaaatca ggttatagac cttcgcttcg ttctccgtca ggttgatcgc agaactccgt
    32281 gcggtcggaa tgatggcgtg gtgcgcatcg acctttttgt catcccaaca gcggttgcgt
    32341 atatctggat ctaccactgg ctgcggcaac agatctggtg catgaacgct gatggcattc
    32401 atcaccgcgt ggcgtccggc aaaatgttct tctggcaaat agcgacaatc ggaacgcgga
    32461 taagtgatta gcttgtgcgt ttcgtacagc ttctggcaga tatcaagcac gttctgcgcg
    32521 ctcagaccaa agcgttttgc cgcttcaatc tgcaacgccg aaagcgaaaa cggcagcggc
    32581 gcggattctg attcccgttt atcgttatag ctggtgacaa tagccggttg accactaatg
    32641 cggttaacca catgctccgc cagtggacga tgtaacaagc gcccttcttc atcctggtac
    32701 ggttcacacg cttcgctcgg ttgccagata gcggtaaacc gctcatcggc aggtgtcacg
    32761 atatgtgctt tgacttcaaa gaagtctttc gccacgaagt tttcaatctc ttcatcgcgg
    32821 cgcaccacca gcccaagcac gggcgtctgc acgcgtccca cggaaagcac gccctgataa
    32881 ccggcattgc gaccgagaat ggtatacgca cgggtcatat tgatgccgta cagccagtcg
    32941 gcacgcgctc gcgccagcgc agaaacgcac agcggtacaa actcactgtt ggaacgaaga
    33001 cggtcgatcg cccgctcaac cgcctgcggg ttcaggtcgt ttatcaagca acgctgcacc
    33061 tgctggcgct tttccggtgc cagttgtaga tagtccagca cttcatccac cagcagttgc
    33121 ccttcgcgat ccgggtcccc ggcgtgaacg atttcgctgg cttcatgcag gtaccgtttg
    33181 atgacgttaa gttgtttggt cacggagggt cggggctgta attgccactt ttccgggaca
    33241 atcggcaaat ccgcaagatt ccagcgcgca tagcggctgt cgtaggcgtc tggctgcgcc
    33301 tgctcaagca ggtgaccgat acaccaggtc accacctgac cattaccgca ctcgataaag
    33361 ccatcgcctt tccggtgcgg tttgggcagg acatcagcaa tggcgcgcgc cagactcggt
    33421 ttttcggcaa taaacaaccg cattgaatta acggatctca accatggcac gaccgccgcg
    33481 tgctggcacc agttcgccaa tcgccgtcag ttcaatgcca aactcggcgg ctgtagcttt
    33541 gacctcattt tcggcttccg gcatgaccgc cagcagcaaa ccgccagaag tttgcggatc
    33601 gcacagcaaa tcgcgcactt cacgcggcat ttcacccatc agatgaccgt agctggcaaa
    33661 gttacgttcg gtgccgccag gcactgcacc caacttaatg tactcttcaa cccccggcaa
    33721 tttcgggatc gcgtcatagt caacgcgcgc ctgcacacct gcgccctgac acatttcgct
    33781 caaatggccc agcaggccaa agcccgttac gtcggtcatc gcttttacgc cttcaatgtt
    33841 ggcaaaggac gcgcctgcga tgttcatccg gcacatcact tccgtcgcca gtccctgatg
    33901 ttctggtttc agcagtgatt ttttctcagc cgtggtaaga acgccgatcc ccagcggttt
    33961 cgtcaggaac agtttacatc cggcttgtgc ggtactgttt ttcttcaccc gctcggtcgg
    34021 tacgatcccc gttaccgcca gaccaaaaat cggctccggc gcatcgatgg agtgaccgcc
    34081 agccagcgca atacccgcct gacggcatgc atagcgtcca ccttcggtta cttcgcgggc
    34141 aatttccggg gaaagtttgt taatcggcca gccgaggatc gcaattgcca taatcggttt
    34201 gccccccatc gcgaagatat cgctgatggc gttagtcgcc gcaatgcggc caaaatcgaa
    34261 aggattatca acgatcggca taaagaagtc ggtggtactg ataacgctgg tgccattgcc
    34321 cagatcgtac accgccgcat cgtcgcgggt ttcattaccc acaagcaaat tcggatcaac
    34381 aaacttcgcc tgctcactgt gcaggatggt ttccaacact tttggggaaa ttttacagcc
    34441 gcaaccagct ccgtggctgt attgggtcaa acgaatcgag ttctcgctca tggacatctc
    34501 ctgtcaatgc aatccgggta tggtaaccct cattccgtga agtgataagt gagagtgtct
    34561 gaattcctgc gcctttgctc acaatccaga cagtttcgcg acaattatca gaaataagtg
    34621 acaaacggcg tcgggtccgg gacgtttatc gacgtagatg ctttcagctg cggcgtgccg
    34681 aggtagagaa aaccgacaat tttatcctgc tcacggcaac cgaatgcttc acgcactacc
    34741 ggactttcag ttaatgcgcc actgcgccag atgccgccaa acccctgggc aacggctgcc
    34801 atttgcatcg ccatgaccgc gcatccggca gacatttcct gttcccatcg cgggacttta
    34861 tgattttctt cgcatttcgc caccaccgtg atgatgagcg gtgcgcggaa cggcgcatta
    34921 cgggctttgt cgatagcttt gtcatcacta ccggcagcaa tcgccccctg ttccagtacg
    34981 gcgctgaaac gctcgcgccc ttccccttca atcacaaaaa aatgccacgg ttgcatggac
    35041 ttatggtccg gcgcgcgcat acccgcacgc aggatgtttt gcagttgttc acccgttggt
    35101 gcgggttcag ccaggcggga ggcgctacgg cgattgatca atagttcgag tgcatccatt
    35161 tgattaactc ctgtcgtgat atttattcac aaaattaaca cgagagtgga ttttgttaca
    35221 gcacagtccg caattcctgc tgacaagtgc cggttgggtc attacgataa ccacatctat
    35281 tgcgcctgtg acaggtgtga ccttaagttg ggagaataca tgcgaaccct ttggcgattt
    35341 attgccggat tttttaaatg gacgtggcgt ctgctgaatt tcgtccgtga aatggtactt
    35401 aacctgttct ttatttttct cgtgctggtt ggtgtgggga tctggatgca ggtcagtggt
    35461 ggtgattcga aagaaacggc cagtcgtggt gcactgctgc tggacatttc tggtgtgatc
    35521 gtcgataaac ccgacagttc tcagcggttt agtaaattaa gccgccaact acttggtgcc
    35581 agttccgatc gtctgcagga aaactcactg tttgatatcg tcaataccat tcgccaggcg
    35641 aaggatgacc gcaatatcac cggtatcgtg atggatctga aaaacttcgc aggcggcgac
    35701 caaccgtcta tgcagtacat cggcaaagcc ctgaaagagt ttcgtgacag cgggaaaccg
    35761 gtttatgccg ttggcgagaa ctacagccag gggcaatatt atctcgccag tttcgccaat
    35821 aaaatttggc tgtcaccgca aggcgtggtg gatttgcacg gttttgctac caacggtctg
    35881 tactacaaat cgttgctgga taagctgaaa gtttccaccc atgtgttccg cgtgggtacg
    35941 tataaatctg ccgttgaacc gtttattcgt gatgacatgt caccggcagc ccgcgaagct
    36001 gacagccgct ggattggtga gctgtggcaa aactatctga atactgttgc cgctaaccgg
    36061 cagatccctg ctcagcaggt attccctggc gcgcaagggt tgcttgaggg tttaaccaaa
    36121 accggtggcg ataccgcgaa atatgcactg gaaaacaagc tggtcgatgc actggcatcg
    36181 agtgcggaaa tcgaaaaagc actgaccaaa gagttcggct ggagtaaggc tgataaaaat
    36241 tatcgcgcca tcagttatta cgattacgca ttgaaaacgc cggcagatac cggtgacagc
    36301 atcggtgtcg tcttcgctaa tggcgcaatt atggatggcg aggaaactca ggggaatgtt
    36361 ggcggtgata ccactgcggc acaaatccgc gacgctcgcc ttgacccgaa agtgaaagcg
    36421 attgtcctgc gtgttaatag cccaggcggc agcgttaccg cgtctgaagt gattcgcgct
    36481 gaactggcag cagcccgggc agcgggtaag cctgtggttg tatcgatggg cggcatggcg
    36541 gcatctggtg gttactggat ttccacgcca gctaattaca ttgtggctaa ccccagcacc
    36601 ctgaccggtt ctattggtat cttcggcgtg atcaccaccg tagaaaatag tctggattcg
    36661 attggtgttc atactgatgg tgtctcaact tcaccgctgg cggatgtttc tatcaccagg
    36721 gcactgccgc cggaagcgca gcagatgatg cagttaagca ttgagaatgg ctataaacgc
    36781 tttatcacgc tggttgctga tgcgcgtcat tcgacgccgg agcagattga taaaatcgcc
    36841 cagggccacg tctggaccgg tcaggacgca aaagctaacg ggctggtcga tagtctcggg
    36901 gatttcgatg atgcggtcgc caaagcagca gagctggcaa aagcgaaaca gtggcatctg
    36961 gaatactacg ttgatgaacc gaccttcttc gacaaagtga tggacaacat gtctggttct
    37021 gtccgggcaa tgttgccaga tgcgttccag gccatgttac ctgcaccgct ggcctcggta
    37081 gcctctaccg ttaaaagtga aagcgacaag ctggcggcgt ttaacgaccc acaaaaccgt
    37141 tatgcgtttt gcctgacctg cgccaacgtg cgttaaatcc tgcaaaaatg tggccgatat
    37201 gctgtcggcc acactcctga atcggactgt ctttcactcc ctctctccac tacattccct
    37261 tgctcattta gcatctgata tggagtcatc agtgaacaag gccccctctt taattgccgc
    37321 tatcgtcctc ggactgggga ttagcgcctg tggttatttt gttggcgatg gtgttaaaca
    37381 tttaaaaacc aacaaccgtt atgtcaatgt gcgcgggctt tctgaaaaag aagttcgcgc
    37441 agatacggcg gaattaacaa ttgcgattaa ctttaagggt aatgttcccg gcgaactgtt
    37501 cccgaaactg gaagaagcgc agaaaaaaat tgttgccgaa ctcaatgctc aggggattaa
    37561 cgagaaagag ataattctgg gccaatggac aagcaagcga acagattcct tttatctgaa
    37621 agatgatccc acaatgccac gctataacgc agacgggtca gtgacgataa aaacccataa
    37681 tgttgctgct gtcgaaaaag tcgtcgcgaa attaaacgaa ctgcaagtcg ctacggatgg
    37741 cgctatcgct gagagcaaag tcgcctatcg ctttaatggt attggcgcac tgcgcgctga
    37801 gatgattgct gccgccacca aagatgcacg caacgccgca ctacaatttg caacggactc
    37861 aggtagccag gtggggtcaa ttagcgatgc ctcacaaggc gtattccaga tctttgccag
    37921 tggcagtgat gaagatgatc ctacggcgat taataaaacg gtacgcgtag tcacgaccgt
    37981 aacctatgcg ttgcaggatt aaccactttt ctccggcggg acactaatcc ctccgtcctg
    38041 ccggactttt ctcatatcca cagagtatcc aggtgcgctc gctgcccctc acgtatatac
    38101 ttttgctctt tcgatatcat tcatatcaat atcatgcaaa agaaatcaat ttacgttgcc
    38161 tacacgggcg ggaccatcgg gatgcagcgt tccgagcagg gttatatacc ggtgtcaggt
    38221 catctacaac gccaactggc gctgatgccg gaattccatc gcccggagat gccagatttc
    38281 accattcatg aatatacgcc gctgatggat tcttcagata tgacgccgga agactggcag
    38341 catattgctg aagatattaa agcgcactat gacgactatg atggttttgt cattctgcac
    38401 ggcaccgaca cgatggcgta taccgcctct gcgctgtcgt tcatgctcga aaatctcggt
    38461 aaaccggtca ttgtgacagg gtcacaaatc ccgctggctg agttacgctc tgacggacaa
    38521 attaatctgc tgaatgcgtt gtacgttgcg gcgaattatc cgatcaacga agtaacactc
    38581 ttttttaata accgactgta tcgcggcaac cgcactacca aagcccatgc cgatggtttt
    38641 gatgcgtttg cctctccaaa ccttcctccg ttactggaag caggtatcca tattcgtcgt
    38701 ttgaatacgc cacccgcccc gcacggtgaa ggggaattga tcgtacatcc aatcacccca
    38761 caaccaattg gcgtagtgac gatttatcca gggatttctg ctgacgtcgt gcgcaatttt
    38821 ctgcgccaac cggtgaaagc attgattctg cgctcctatg gcgtgggtaa tgcgccacaa
    38881 aacaaagcct tcctgcagga attacaagaa gccagcgatc gcggtattgt ggtggtcaac
    38941 ctgacacaat gtatgtccgg taaagtgaat atgggtggtt atgccaccgg taacgccctc
    39001 gcccatgctg gcgttattgg cggtgcagat atgactgtag aagccacgct aaccaaactg
    39061 cattacctgc ttagccagga actggatact gaaaccattc gcaaggccat gagccaaaac
    39121 ctgcgcggcg aactgacgcc ggatgattaa ggagactgca atgccccctc gcgccctgtt
    39181 actggtcgat ttacaaaatg atttctgtgc tggtggcgcg ctcgccgtgc cggaaggtga
    39241 cagtacggtg gatgtcgcta accgcctgat tgactggtgc cagtcgcgcg gtgaagcggt
    39301 tatcgccagt caggactggc acccggcgaa tcacggcagt tttgccagcc agcacggtgt
    39361 agagccttat acgccaggcc aactcgacgg tttgccacaa accttctggc cagatcactg
    39421 tgtgcagaac agtgaaggcg cacaattaca tccgttactg aaccaaaaag cgatcgcagc
    39481 ggtgttccat aaaggcgaaa atcctttagt tgacagttac agtgcctttt ttgataacgg
    39541 ccgtcggcag aaaacctctc tcgatgactg gttacgcgat catgaaatcg atgaattgat
    39601 cgttatgggc ctggctactg actattgcgt gaagtttacc gtgctggacg cgttacagtt
    39661 aggttataag gtaaacgtga ttaccgatgg ttgtcgtggc gtgaatatcc agccccagga
    39721 cagtgcgcac gcgtttatgg agatgtcagc agctggggca acgctatata cgctggcaga
    39781 ctgggaagag acgcaggggt aattttacgc tggcctacaa ttctgtactg gcattgtagg
    39841 ccaaataaaa cacgtcagtg gcacatctgg caattgatgc catcaacgaa agatttaatt
    39901 tacctcagaa atctcttcca acgacacctt ccgcgtttcg atgccaaaaa tggagagaac
    39961 cagcgcacag agcaataaca tgacaccaag taccataaac accgtgatcg acccataatg
    40021 tgttaataat gccgcaacgc catagggcgt gaagactgcg acgatccgtc cgacggcatt
    40081 aacgaaaccc gaaccgcgca ggcgtaaatg cgttggccaa agctccggga tataaaccgc
    40141 cgacgcgaag caaacataca tgtataaaaa gaagatcatc accaggccat agattaaaat
    40201 cgcccactct gtagtctgaa ttgaatagat atagcctaac actgcaataa taataagtaa
    40261 ggtggagcca aataaccgac gaggaaaatg atcaataatt aatgccgcaa taaatattcc
    40321 taccggagcg ccaatcataa taacagcggt cattaatatt gatttatcga catcaatgcc
    40381 ggagttaaca aatatggtcg gtatccatac ggtgatggta taaagcgaaa tgttcattgc
    40441 aattaaaaca gtaatcgcga ctaatgtacg tcgtaacatt tcacctttaa acaggagcca
    40501 gaaagtaccc ttgattactt taaccttgct gttgctctga tacgaagtaa gcgggggtaa
    40561 acgaatactc ttctctcttt caatttgctg ctctacttca cgaagttggc attctgcacc
    40621 tgcgatttgc cctttccctg ccagccatcg tggcgactca ataaagtatt tacctgagag
    40681 aaaccaggct aacagtatgc caataccacc caacagaaac attatccgcc aactaaaaaa
    40741 agcgataacc accacaccta tcgccgcaga cagcatgggc gaccagttac caacaaatga
    40801 gagccgcgcg gaccattttc cacgcaccgt cgcggggata aactccgtaa atgaggcata
    40861 gccaaccata atcagcgccc ccattcctgt tcccatcagg aagcgaaaga agataagcca
    40921 gtacatatca gggacaaaag cggcccctgt tgcagcaata ccgacgatga gaagatttat
    40981 gcgaaacgcc ctgcgccgcc caaagtaatc accgatgaac ccaccggtaa gagagccgat
    41041 gaaataacca aacattaatg ccgaggtaaa ggcggcattg aggaaattat ttgaccatcc
    41101 attgcttacc agctttgcta agacgacatt accggagtaa ctcaaaaacc c
//
LOCUS       DAZFAB010000031        40897 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZFAB010000031 DAZFAB010000000
VERSION     DAZFAB010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40897
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.031"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            159..1727
                     /gene="cydA"
                     /locus_tag="ACHJWA_001943"
     CDS             159..1727
                     /gene="cydA"
                     /locus_tag="ACHJWA_001943"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFO4308551.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            1743..2882
                     /gene="cydB"
                     /locus_tag="ACHJWA_001944"
     CDS             1743..2882
                     /gene="cydB"
                     /locus_tag="ACHJWA_001944"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO4308552.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            2897..3010
                     /gene="cydX"
                     /locus_tag="ACHJWA_001945"
     CDS             2897..3010
                     /gene="cydX"
                     /locus_tag="ACHJWA_001945"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFO4308553.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            3010..3303
                     /gene="ybgE"
                     /locus_tag="ACHJWA_001946"
     CDS             3010..3303
                     /gene="ybgE"
                     /locus_tag="ACHJWA_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFO4308554.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            3453..3857
                     /gene="ybgC"
                     /locus_tag="ACHJWA_001947"
     CDS             3453..3857
                     /gene="ybgC"
                     /locus_tag="ACHJWA_001947"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO4308555.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            3854..4546
                     /gene="tolQ"
                     /locus_tag="ACHJWA_001948"
     CDS             3854..4546
                     /gene="tolQ"
                     /locus_tag="ACHJWA_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFO4308556.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            4550..4978
                     /gene="tolR"
                     /locus_tag="ACHJWA_001949"
     CDS             4550..4978
                     /gene="tolR"
                     /locus_tag="ACHJWA_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFO4308557.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            5043..6257
                     /gene="tolA"
                     /locus_tag="ACHJWA_001950"
     CDS             5043..6257
                     /gene="tolA"
                     /locus_tag="ACHJWA_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFO4308558.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADDKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAAAEKAAA
                     DKKAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAAAAKAAAEKAAAAKAAAE
                     ADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAGQIKSAIESK
                     FYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKPPSQAVY
                     EVFKNAPLDFKP"
     gene            6390..7682
                     /gene="tolB"
                     /locus_tag="ACHJWA_001951"
     CDS             6390..7682
                     /gene="tolB"
                     /locus_tag="ACHJWA_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFO4308559.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            7717..8238
                     /gene="pal"
                     /locus_tag="ACHJWA_001952"
     CDS             7717..8238
                     /gene="pal"
                     /locus_tag="ACHJWA_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFO4308560.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            8248..9039
                     /gene="cpoB"
                     /locus_tag="ACHJWA_001953"
     CDS             8248..9039
                     /gene="cpoB"
                     /locus_tag="ACHJWA_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFO4308561.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVIQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSGDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            9204..9279
                     /locus_tag="ACHJWA_001954"
     tRNA            9204..9279
                     /locus_tag="ACHJWA_001954"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9237..9239,aa:Lys,seq:ttt)
     gene            9315..9390
                     /locus_tag="ACHJWA_001955"
     tRNA            9315..9390
                     /locus_tag="ACHJWA_001955"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9348..9350,aa:Val,seq:tac)
     gene            9393..9468
                     /locus_tag="ACHJWA_001956"
     tRNA            9393..9468
                     /locus_tag="ACHJWA_001956"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9426..9428,aa:Lys,seq:ttt)
     gene            9520..9595
                     /locus_tag="ACHJWA_001957"
     tRNA            9520..9595
                     /locus_tag="ACHJWA_001957"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9553..9555,aa:Val,seq:tac)
     gene            9599..9674
                     /locus_tag="ACHJWA_001958"
     tRNA            9599..9674
                     /locus_tag="ACHJWA_001958"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9632..9634,aa:Lys,seq:ttt)
     gene            9821..9896
                     /locus_tag="ACHJWA_001959"
     tRNA            9821..9896
                     /locus_tag="ACHJWA_001959"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9854..9856,aa:Lys,seq:ttt)
     gene            10174..11217
                     /gene="nadA"
                     /locus_tag="ACHJWA_001960"
     CDS             10174..11217
                     /gene="nadA"
                     /locus_tag="ACHJWA_001960"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFO4308562.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKKTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNYEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            11255..11974
                     /gene="pnuC"
                     /locus_tag="ACHJWA_001961"
     CDS             11255..11974
                     /gene="pnuC"
                     /locus_tag="ACHJWA_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFO4308563.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVIIMQALGLQVAMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(11971..12912)
                     /gene="zitB"
                     /locus_tag="ACHJWA_001962"
     CDS             complement(11971..12912)
                     /gene="zitB"
                     /locus_tag="ACHJWA_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFO4308564.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVIGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(13026..13406)
                     /gene="ybgS"
                     /locus_tag="ACHJWA_001963"
     CDS             complement(13026..13406)
                     /gene="ybgS"
                     /locus_tag="ACHJWA_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFO4308565.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHPDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            13722..14774
                     /gene="aroG"
                     /locus_tag="ACHJWA_001964"
     CDS             13722..14774
                     /gene="aroG"
                     /locus_tag="ACHJWA_001964"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFO4308566.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            complement(14940..15692)
                     /gene="gpmA"
                     /locus_tag="ACHJWA_001965"
     CDS             complement(14940..15692)
                     /gene="gpmA"
                     /locus_tag="ACHJWA_001965"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFO4308567.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(15894..16934)
                     /gene="galM"
                     /locus_tag="ACHJWA_001966"
     CDS             complement(15894..16934)
                     /gene="galM"
                     /locus_tag="ACHJWA_001966"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFO4308568.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGKQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNYLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLHPGEE
                     YSSLTEYQFIAE"
     gene            complement(16928..18076)
                     /gene="galK"
                     /locus_tag="ACHJWA_001967"
     CDS             complement(16928..18076)
                     /gene="galK"
                     /locus_tag="ACHJWA_001967"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFO4308569.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            complement(18080..19126)
                     /gene="galT"
                     /locus_tag="ACHJWA_001968"
     CDS             complement(18080..19126)
                     /gene="galT"
                     /locus_tag="ACHJWA_001968"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFO4308570.1"
                     /translation="MTQFNPVDHPHRRYNQLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            complement(19136..20152)
                     /gene="galE"
                     /locus_tag="ACHJWA_001969"
     CDS             complement(19136..20152)
                     /gene="galE"
                     /locus_tag="ACHJWA_001969"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFO4308571.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            complement(20414..21886)
                     /gene="modF"
                     /locus_tag="ACHJWA_001970"
     CDS             complement(20414..21886)
                     /gene="modF"
                     /locus_tag="ACHJWA_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFO4308572.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITPLLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYHYALTKIN"
     gene            complement(21954..22742)
                     /gene="modE"
                     /locus_tag="ACHJWA_001971"
     CDS             complement(21954..22742)
                     /gene="modE"
                     /locus_tag="ACHJWA_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFO4308573.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            22871..23020
                     /gene="acrZ"
                     /locus_tag="ACHJWA_001972"
     CDS             22871..23020
                     /gene="acrZ"
                     /locus_tag="ACHJWA_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFO4308574.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            23187..23960
                     /gene="modA"
                     /locus_tag="ACHJWA_001973"
     CDS             23187..23960
                     /gene="modA"
                     /locus_tag="ACHJWA_001973"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO4308575.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            23960..24649
                     /gene="modB"
                     /locus_tag="ACHJWA_001974"
     CDS             23960..24649
                     /gene="modB"
                     /locus_tag="ACHJWA_001974"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFO4308576.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            24652..25710
                     /gene="modC"
                     /locus_tag="ACHJWA_001975"
     CDS             24652..25710
                     /gene="modC"
                     /locus_tag="ACHJWA_001975"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFO4308577.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(25711..26529)
                     /gene="ybhA"
                     /locus_tag="ACHJWA_001976"
     CDS             complement(25711..26529)
                     /gene="ybhA"
                     /locus_tag="ACHJWA_001976"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFO4308578.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDEMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            26684..27679
                     /gene="pgl"
                     /locus_tag="ACHJWA_001977"
     CDS             26684..27679
                     /gene="pgl"
                     /locus_tag="ACHJWA_001977"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFO4308579.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNIDHRGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(27720..28673)
                     /gene="ybhD"
                     /locus_tag="ACHJWA_001978"
     CDS             complement(27720..28673)
                     /gene="ybhD"
                     /locus_tag="ACHJWA_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4308580.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            28857..29909
                     /gene="ybhH"
                     /locus_tag="ACHJWA_001979"
     CDS             28857..29909
                     /gene="ybhH"
                     /locus_tag="ACHJWA_001979"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFO4308581.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEKRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGGLDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            29985..31418
                     /gene="ybhI"
                     /locus_tag="ACHJWA_001980"
     CDS             29985..31418
                     /gene="ybhI"
                     /locus_tag="ACHJWA_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFO4308582.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESSVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            31601..33862
                     /gene="ybhJ"
                     /locus_tag="ACHJWA_001981"
     CDS             31601..33862
                     /gene="ybhJ"
                     /locus_tag="ACHJWA_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFO4308583.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLINCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVAALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGAAQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     VEAPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(34003..35286)
                     /gene="ybhC"
                     /locus_tag="ACHJWA_001982"
     CDS             complement(34003..35286)
                     /gene="ybhC"
                     /locus_tag="ACHJWA_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFO4308584.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWGDAVISNRPFAGNTGSVD
                     DSDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(35421..36491)
                     /locus_tag="ACHJWA_001983"
     CDS             complement(35421..36491)
                     /locus_tag="ACHJWA_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4308585.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMS
                     KIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHI
                     TTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCE
                     MKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTR
                     REPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHK
                     SDTMASQYRDDRGREWDKIEIK"
     gene            complement(36469..36687)
                     /locus_tag="ACHJWA_001984"
     CDS             complement(36469..36687)
                     /locus_tag="ACHJWA_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO4308586.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTGSLLKRIRNGKKAKS"
     gene            complement(36727..36894)
                     /locus_tag="ACHJWA_001985"
     CDS             complement(36727..36894)
                     /locus_tag="ACHJWA_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4308587.1"
                     /translation="MHFRVTGEWNGEPFNRVIEAENINDCYDHWMIWAQIAHADITNI
                     RIEELKEHQAA"
     gene            37137..37739
                     /locus_tag="ACHJWA_001986"
     CDS             37137..37739
                     /locus_tag="ACHJWA_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4308588.1"
                     /translation="MSYFLRKKWMVNLSGAGKILWALNMKKDSYPYLICMTVLGLIFI
                     FLFFWWRADIYRVTFLNQSISHYYILFSMGIAFLLSLFWVKKGIVKQSGWKSLSAYLK
                     VYAGMCIFAGFFLIIPLTTLTYFLPGETSSYVAPYRYTSGSSKSCSGAEVDDPDLHEN
                     IRICYPYGNYEYDNIIYVEKKINTLGAVVTYAQTARDDTE"
     gene            complement(37950..38171)
                     /locus_tag="ACHJWA_001987"
     CDS             complement(37950..38171)
                     /locus_tag="ACHJWA_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO4308589.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNHQAISATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEDLELISKQRGSK"
     gene            complement(38270..38485)
                     /locus_tag="ACHJWA_001988"
     CDS             complement(38270..38485)
                     /locus_tag="ACHJWA_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO4308590.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQPFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            complement(38562..38753)
                     /locus_tag="ACHJWA_001989"
     CDS             complement(38562..38753)
                     /locus_tag="ACHJWA_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFO4308591.1"
                     /translation="MHKASPVELRTSIEMAHSLAQIGVRFVPIPVETDEEFHTLATSL
                     SQKLEMMVAKAEADERDLV"
     gene            complement(38726..38908)
                     /locus_tag="ACHJWA_001990"
     CDS             complement(38726..38908)
                     /locus_tag="ACHJWA_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFO4308592.1"
                     /translation="MKHPHDNIRVGTITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEE
                     INNKREAICTKHLLLS"
     gene            complement(38905..39585)
                     /locus_tag="ACHJWA_001991"
     CDS             complement(38905..39585)
                     /locus_tag="ACHJWA_001991"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFO4308593.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVVERDEKYMAGFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            complement(39582..40253)
                     /gene="bet"
                     /locus_tag="ACHJWA_001992"
                     /pseudo
     CDS             complement(39582..40253)
                     /gene="bet"
                     /locus_tag="ACHJWA_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
     gene            40283..40465
                     /locus_tag="ACHJWA_001993"
     CDS             40283..40465
                     /locus_tag="ACHJWA_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO4308594.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHCARKTR"
     gene            40462..40632
                     /locus_tag="ACHJWA_001994"
     CDS             40462..40632
                     /locus_tag="ACHJWA_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HFO4308595.1"
                     /translation="MIDQNRSYEQESVERALTCANCGQKLHVLEVHVCEHCCAELMSD
                     SNSSMHEEKDDG"
     gene            40625..>40897
                     /locus_tag="ACHJWA_001995"
     CDS             40625..>40897
                     /locus_tag="ACHJWA_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO4308596.1"
                     /translation="MAKPARRRCKNDECREWFHPAFANQWWCSPECGTKIALERRSKE
                     REKAEKAEKAAEKKRRREEQKQKDKLKIQKLALKPRSYWIKQAQQAV"
BASE COUNT         9832 a         9965 c        10611 g        10489 t
ORIGIN      
        1 tcattaacat atttgtaact tttttatcat tgtcttttcc tcttttgatg aaatggcaac
       61 gatagaggcg agcaaataat tttgtattgg ggcatgcgtg tgaccctttc taacggggtt
      121 cactctcgga gtcttcatgc gatgagcaag gagtcatgat gttagatata gtcgaactgt
      181 cgcgcttaca gtttgccttg accgcgatgt accacttcct ttttgtgcca ctgacgctcg
      241 gtatggcgtt cctgctggcc attatggaaa cggtctacgt cctttccggc aaacagattt
      301 ataaagatat gaccaagttc tggggcaagt tgtttggtat caacttcgct ctgggtgtgg
      361 ctaccggtct gaccatggag ttccagttcg ggactaactg gtcttactat tcccactatg
      421 taggggatat cttcggtgcg ccgctggcaa tcgaaggtct gatggccttc ttcctcgaat
      481 ccacctttgt aggtctgttc ttcttcggtt gggatcgtct gggtaaagtt cagcatatgt
      541 gtgtcacctg gctggtggcg ctcggttcaa acctgtccgc actgtggatt ctggttgcga
      601 acggctggat gcaaaaccca atcgcgtccg atttcaactt tgaaactatg cgtatggaga
      661 tggtgagctt ctccgagctg gtgcttaacc cggttgctca ggtgaaattc gttcacactg
      721 tagcgtctgg ttatgtgact ggcgcgatgt tcatcctcgg tatcagcgca tggtatatgc
      781 tgaaaggtcg tgacttcgcc ttcgctaaac gctcctttgc tatcgctgcc agcttcggta
      841 tggctgccgt tctgtctgtt attgttctgg gtgatgaatc cggctacgaa atgggcgacg
      901 tgcagaaaac caaactggct gctattgaag ctgagtggga aacgcaacct gcgcctgctg
      961 cctttactct gttcggcatt cctgatcagg aagaggagac gaacaaattt gcgatccaga
     1021 tcccttacgc gctgggcata attgcaacgc gttccgtgga tactccagtt atcggcctga
     1081 aagaactgat ggtgcagcat gaagaacgca ttcgtaacgg gatgaaggcg tactctctgc
     1141 tcgaacaact gcgttctggt tctaccgacc aggcggttcg tgaccagttc aatagcatga
     1201 agaaggacct cggttacggt ctgctgctga aacgctatac gccaaacgtg gctgatgcga
     1261 ctgaagcgca gattcaacag gcaaccaaag actccattcc gcgtgtagcg ccgctgtact
     1321 tcgcgttccg tatcatggtg gcgtgtggct tcctgctgct ggcaatcatc gcgctctctt
     1381 tctggagtgt catccgcaac cgcattggcg agaaaaaatg gctcctgcgc gccgcgctgt
     1441 acggtattcc gctgccgtgg attgctgtag aagcgggctg gttcgtggct gaatatggcc
     1501 gccaaccgtg ggctatcggt gaagtgctgc cgacagctgt ggcgaactcg tcactgaccg
     1561 caggcgatct catcttctca atggtgctga tttgcggcct gtataccctg ttcctggtgg
     1621 cagaattgtt cttaatgttc aagtttgcac gcctcggccc aagcagcctg aaaaccggtc
     1681 gctatcactt tgagcagtct tccacgacta ctcagccggc acgctaagac aggagtcgtc
     1741 aaatgatcga ttatgaagta ttgcgtttta tctggtggct gctggttggc gttctgctga
     1801 ttggttttgc agtcactgat ggtttcgaca tgggggtggg catgctcacc cgtttcctcg
     1861 gtcgtaacga caccgagcgt cgaattatga ttaactccat cgcaccacac tgggacggta
     1921 accaggtttg gctgatcacc gcaggcggcg cactgtttgc tgcctggccg atggtctatg
     1981 ccgctgcgtt ctccggcttc tatgtggcga tgatcctcgt gctggcgtct ttgttcttcc
     2041 gtccggtcgg ttttgactac cgctccaaga ttgaagaaac ccgctggcgt aacatgtggg
     2101 actggggcat cttcattggt agcttcgttc cgccgttggt gattggtgta gcgttcggta
     2161 acctgttgca gggcgtaccg ttcaacgttg atgaatatct gcgtctgtac tacaccggta
     2221 acttcttcca gctgcttaac ccgttcggcc tgctggcagg cgtggtgagc gtagggatga
     2281 tcattactca gggcgcaacc tatctgcaaa tgcgtaccgt gggcgaactg cacctgcgta
     2341 cccgtgcaac ggctcaggtg gcggcactgg tgacactggt ctgtttcgca ctggctggcg
     2401 tatgggtgat gtacggtatc gatggttatg tcgtgaaatc gacaatggac cattacgcag
     2461 cctctaaccc gctgaataaa gaagtggttc gtgaagctgg cgcatggctg gttaacttca
     2521 acaacacgcc aattctgtgg gctattccgg cactgggtgt ggttctgccg ctgctgacca
     2581 tcctgactgc acgtatggat aaagccgcgt gggcgtttgt gttctcctcc ctgacgctgg
     2641 cctgcatcat cctgactgcc ggtatcgcaa tgttcccgtt tgtgatgccg tccagcacca
     2701 tgatgaacgc aagtctgaca atgtgggatg caacttccag ccagctgaca cttaacgtca
     2761 tgacctgggt tgcggtggtt ctggtaccga tcattctgct ctacaccgcc tggtgttact
     2821 ggaaaatgtt cggtcgtatc accaaagaag atattgaacg taacacccac tctctgtact
     2881 aagtaaggag ctaaaaatgt ggtatttcgc atggattctg ggaacgcttc ttgcctgttc
     2941 gtttggggta atcaccgcgc tggcgcttga gcacgtcgaa tcaggcaaag ccggtcaaga
     3001 agacatctga tgagtaagat tatcgcaacc ttgtatgcgg taatggacaa gcgccccctg
     3061 cgggcgcttt ccttcgtgat ggcgcttctg ttagcaggat gtatgttttg ggacccatct
     3121 cgtttcgccg cgaagaccag tgaactggaa atctggcatg gtttattgct gatgtgggcc
     3181 gtctgtgctg gtgtgattca cggcgtgggc tttcgtccgc agaaggttct ttggcaaggg
     3241 attttttgcc cattgcttgc cgatattgtt ctcattgtcg ggctgatttt cttcttcttt
     3301 taaatcagaa ttctctttaa aaaattatgg gccgtgccag gcccataaat ttttacgctc
     3361 ccttaacttg ccctcattcc caaacctcaa tcgcgcgcgt atagtagcag cgtttaaaag
     3421 ctctaacttt tgttgcatta ccgggatgta aagtgaatac aacgctgttt cgatggccgg
     3481 ttcgcgtcta ctatgaagat accgatgccg gtggtgtggt gtaccacgcc agttacgtcg
     3541 ctttttatga aagagcacgc acagagatgc tgcgtcatca tcacttcagt caacaggcgc
     3601 tgatggctga acgcgttgcc tttgtggtac gtaaaatgac ggtggaatat tacgcacctg
     3661 cgcggctcga cgatatgctc gaaatacaga ctgaaataac atcaatgcgt ggcacctctt
     3721 tggttttcac gcaacgtatt gtcaacgccg agaatacttt gctgaatgaa gcagaggttc
     3781 tggttgtttg cgttgaccca ctcaaaatga agcctcgtgc gcttcccaag tctattgtcg
     3841 cggagtttaa gcagtgactg acatgaatat ccttgatttg ttcctgaagg ctagccttct
     3901 ggttaaactt atcatgttga ttttgattgg tttttcaatc gcatcctggg ccattattat
     3961 ccagcggacc cgtattctta acgcagcggc gcgcgaagcc gaagcgtttg aagataaatt
     4021 ctggtctgga atcgaactct ctcgcctcta tcaagagagc caggggaaac gggataatct
     4081 gactggttcg gaacaaatct tttacagcgg gttcaaagaa tttgtgcgcc tgcatcgtgc
     4141 caatagccat gcgccggaag ccgtagtgga aggggcgtcg cgtgcgatgc gtatttccat
     4201 gaaccgcgaa cttgaaaatc tggaaacgca cattccgttc ctcggtacgg ttggctccat
     4261 cagcccgtat attggtctgt ttggtacggt ctgggggatc atgcacgcct ttatcgccct
     4321 cggggcggta aaacaagcaa cactgcaaat ggttgcgccc ggtatcgcag aagcgttgat
     4381 tgcgacggca attggtctgt tcgccgctat tccggcggta atggcttata accgtctcaa
     4441 ccaacgtgta aacaaactgg aactgaatta cgacaacttt atggaagagt ttaccgcgat
     4501 tctgcaccgc caggcgttta ccgttagcga gagcaacaag gggtaagcca tggccagagc
     4561 gcgtggacga ggtcgtcgcg atctcaagtc cgaaatcaac attgtaccgt tgctggacgt
     4621 actgctggtg ctgttgctga tctttatggc gacagcgccc atcatcaccc agagcgtgga
     4681 ggtcgatttg ccagacgcta ctgaatcaca ggcggtgagc agtaacgata atccgccagt
     4741 gattgttgaa gtgtctggta ttggtcagta caccgtggtg gttgagaaag atcgcctgga
     4801 gcgtttacca ccagagcagg tggtggcgga agtgtccagc cgtttcaagg ccaacccgaa
     4861 aacggtcttt ctgatcggtg gcgcaaaaga tgtgccttac gatgaaataa ttaaagcact
     4921 gaacttgtta catagtgcgg gtgtgaaatc ggttggttta atgacgcagc ctatctaaac
     4981 atctgcgttt cccttgcttg aaagagagcg ggtaacaggc gaacagtttt tggaaaccga
     5041 gagtgtcaaa ggcaaccgaa caaaacgaca agctcaagcg ggcgataatt atttcagcag
     5101 tgctgcatgt catcttattt gcggcgctga tctggagttc gttcgatgag aatatagaag
     5161 cttcagctgg aggcggcggt ggttcgtcca tcgacgctgt catggttgat tcaggtgcgg
     5221 tagttgagca gtacaaacgc atgcaaagcc aggaatcaag cgcgaagcgt tctgatgagc
     5281 agcgcaagat gaaggaacag caggctgctg aagaactgcg tgagaaacaa gcggctgaac
     5341 aggaacgcct gaagcaactt gagaaagagc ggttagctgc tcaggaacag aaaaagcagg
     5401 ctgaagaagc cgcaaaacag gccgagttaa agcagaagca agcggaagag gcggcagcga
     5461 aagcggcggc agatgctaaa gcgaaggccg aagcggatga taaagctgcg gaagaagcag
     5521 cgaagaaagc ggctgcagac gcgaagaaaa aagcagaagc agaagccgcc aaagccgcag
     5581 ccgaagcgca gaaaaaagcc gaggcagcag ctgcggcgct gaagaagaaa gcggaagcgg
     5641 cagaagcagc tgcagctgaa gcaagaaaga aagcggcagc agagaaagct gcagccgaca
     5701 aaaaagcagc agagaaagct gcagccgaca aaaaagcagc agaaaaagcg gctgctgaaa
     5761 aggcagcagc tgataagaaa gcagcggcag aaaaagccgc cgcagacaaa aaagcggcag
     5821 ctgcaaaagc agcagctgaa aaagccgctg cagcaaaagc tgccgcggag gcagatgata
     5881 ttttcggtga gctaagctct ggtaagaatg caccgaaaac ggggggaggg gcgaaaggga
     5941 acaatgcttc gcctgccggg agtggtaata ctaaaaacaa tggcgcatca ggggccgata
     6001 tcaataacta tgccgggcag attaaatctg ctatcgaaag taagttctat gacgcatcgt
     6061 cctatgcagg caaaacctgt acgctgcgca taaaactggc acccgatggt atgttactgg
     6121 atatcaaacc tgaaggtggc gatcccgcac tttgtcaggc tgcgttggca gcagctaaac
     6181 ttgcgaagat cccgaaacca ccaagccagg cagtatatga agtgttcaaa aacgcgccat
     6241 tggacttcaa accgtaatcg cgatgttgac tgttccgacg gtcaacatca ggcaccggtt
     6301 gccacggggt tctggtagtt ttgtgtattt tagtttgtta acattctgct aaattatcgt
     6361 gggccatcgg tccagataag ggagatatga tgaagcaggc attacgagta gcatttggtt
     6421 ttctcatact gtgggcatca gttctgcatg ctgaagtccg cattgtgatc gacagcggtg
     6481 tagattccgg tcgtcctatt ggagttgttc ctttccagtg ggcgggccct ggtgcggcac
     6541 ctgaagatat tggcggcatc gttgctgccg acttgcgtaa cagcggtaaa tttaatccgt
     6601 tagatcgcgc tcgtctgcca cagcagccgg gtagtgcgca ggaagtacaa ccagctgcat
     6661 ggtccgcgct gggtattgac gctgtagttg tcggtcaggt cactccgaat ccggatggtt
     6721 cttacaatgt tgcttatcaa cttgttgaca ctggcggcgc accgggtact gtacttgctc
     6781 agaactcgta caaagtgaac aagcagtggc tgcgttatgc tggtcatacc gccagtgatg
     6841 aagtgtttga aaaactgacc ggtattaaag gtgcgttccg tacccgtatt gcctacgttg
     6901 ttcagaccaa cggcggtcag ttcccgtatg aactgcgcgt atctgactat gacggttaca
     6961 accagtttgt cgttcaccgt tcaccacagc cgctgatgtc cccggcgtgg tcaccagacg
     7021 gttctaaact ggcttatgtg accttcgaaa gcggtcgttc cgcgctggtt atacaaacgc
     7081 tggcaaacgg cgctgtacgt caggtggctt cattcccgcg tcacaacggt gcgcctgcat
     7141 tctcgccaga cggcagcaaa ctggcattcg ccttgtcgaa aaccggtagc ctgaacctgt
     7201 acgtaatgga tttggcttct ggtcagatcc gccaggtgac tgatggtcgc agtaacaata
     7261 ccgaaccgac ctggttcccg gacagccaga acctggcatt tacttctgac caggccggtc
     7321 gtccacaggt ttataaagtg aatatcaacg gcggtgcgcc acaacgtatt acctgggaag
     7381 gttcgcagaa ccaggatgcg gatgtcagca gcgacggtaa atttatggta atggtcagct
     7441 ccaatggtgg gcagcagcac attgccaaac aagatctggc aacgggaggc gtacaagttc
     7501 tgtcgtccac gttcctggat gaaacgccaa gtctggcacc taacggcact atggtaatct
     7561 acagctcttc tcaggggatg ggatccgtgc tgaatttggt ttctacagat gggcgtttca
     7621 aagcgcgtct tccggcaact gatggacagg tcaaattccc tgcctggtcg ccgtatctgt
     7681 gataataatt aattgaatag taaaggaatc attgaaatgc aactgaacaa agtgctgaaa
     7741 gggctgatga ttgctctgcc tgttatggca attgcggcat gttcttccaa caagaacgcc
     7801 agcaatgacg gcagcgaagg catgctgggt gccggcactg gtatggatgc gaacggcggc
     7861 aacggcaaca tgtcttccga agagcaggct cgtctgcaaa tgcaacagct gcagcagaac
     7921 aacatcgttt acttcgatct ggacaagtac gatatccgtt ctgacttcgc tcaaatgctg
     7981 gatgcacatg caaacttcct gcgtagcaac ccgtcttaca aagtcaccgt agaaggtcac
     8041 gcggacgaac gtggtactcc ggaatacaac atctccctgg gtgaacgtcg tgcgaacgcc
     8101 gttaagatgt acctgcaggg taaaggcgtt tctgcagacc agatctccat cgtttcttac
     8161 ggtaaagaaa aacctgcagt actgggtcat gacgaagcgg catactccaa aaaccgtcgt
     8221 gcggtactgg tttactaaga gaattgcatg agcagtaact tcagacatca actattgagt
     8281 ctgtcgttac tggttggtat agcggccccc tgggccgctt ttgctcaggc accaatcagt
     8341 agtgtcggct caggctcggt cgaagaccgc gtcattcaac ttgagcgtat ttctaatgct
     8401 cacagccagc ttttaaccca actccagcaa caactctctg ataatcaatc cgatattgat
     8461 tccctgcgtg gtcagattca ggaaaatcag tatcaactga atcaggtcgt ggagcggcaa
     8521 aagcagatcc tgttgcagat cgacagcctc agcagcggtg gtgcagcggc gcaatcaacc
     8581 agcggtgatc aaagcggtgc ggcggcatca acgacgccga cagctgatgc tggtactgcg
     8641 aatgctggcg cgccggtgaa aagcggtgat gcaaacacgg attacaatgc agctattgcg
     8701 ctggtgcagg ataaatcccg ccaggatgac gcaatggtgg catttcagaa tttcatcaaa
     8761 aattaccctg attcaactta cctgccaaac gccaattatt ggctgggtca gttaaactac
     8821 aacaagggta aaaaagatga tgcggcgtac tattttgctt cggtagtgaa aaactatccg
     8881 aagtcaccaa aggctgcaga tgcgatgttt aaagtcggcg tcatcatgca ggacaaaggt
     8941 gacaccgcaa aagcgaaagc cgtataccag caggttatca gtaaataccc tggtactgat
     9001 ggcgctaaac aggcacaaaa acgtctgaac gcgatgtaat gcataacaca cgaccagaag
     9061 tcgcattatt tctggtcgtg tcgtgcgaat cataagcagt tgagtgatct acatcgaaat
     9121 ttttgttgcg ctcaagtctg aaatcagtaa tatatgccgc cgttgccacg ggatatcaaa
     9181 caaaccgaaa gcaacgaaaa agtgggtcgt tagctcagtt ggtagagcag ttgactttta
     9241 atcaattggt cgcaggttcg aatcctgcac gacccaccac taacctagtt agttgtagta
     9301 tccagcgtaa tatcgggtga ttagctcagc tgggagagca cctcccttac aaggaggggg
     9361 tcggcggttc gatcccgtca tcacccacca ccgggtcgtt agctcagttg gtagagcagt
     9421 tgacttttaa tcaattggtc gcaggttcga atcctgcacg acccaccact aattacggtg
     9481 ggttcggtgg aagtagtttg tagtatccag cgcagtatcg ggtgattagc tcagctggga
     9541 gagcacctcc cttacaagga gggggtcggc ggttcgatcc cgtcatcacc caccactcgg
     9601 gtcgttagct cagttggtag agcagttgac ttttaatcaa ttggtcgcag gttcgaatcc
     9661 tgcacgaccc accaatttta acatcaaact cagatgttaa gcgtgaagga taacgttgcg
     9721 tcagcaacgg cccgtagggc aagcgaagcg agtcatcctg cacgacccac caattttaaa
     9781 gattggttcc gagtagaaat ctttcaggta acacccgtat gggtcgttag ctcagttggt
     9841 agagcagttg acttttaatc aattggtcgc aggttcgaat cctgcacgac ccaccaatgt
     9901 aaaaaagcgc cctaaaggcg cttttttgct attcaggcat ctctcaattt caattcttga
     9961 ccctgatgac atactccgcg cttgctgcgt aaacaacttt acgtcggagg aataagacgc
    10021 aaagtgtggc attcggcatc tcatacgtaa ttcgagtgac ttttccccac cattcgacta
    10081 tcttgtttag catataaaac aaattacacc gataacagcg aatatcacgc taatgtcggt
    10141 tttaacgtta agcctgtaaa acgagatggt aagatgagcg tgatgtttga tccagacacg
    10201 gcgatttatc ctttcccccc gaagccgacg ccgttaagca ttgatgaaaa agcgtattac
    10261 cgcgagaaga taaaacgtct gctaaaagaa cgtaatgcgg tgatggttgc ccactactat
    10321 accgatcccg aaattcaaca actggcagaa gaaaccggtg gctgtatttc tgattctctg
    10381 gaaatggcgc gcttcggtgc aaagcatccc gcttctacgt tgttagtcgc tggggtgaga
    10441 tttatgggag aaaccgccaa aattctcagt ccggaaaaaa caattctgat gccgacactt
    10501 caggctgagt gttcactgga tctcggctgc cctgttgaag aatttaacgc attttgcgat
    10561 gcccatcccg atcgtactgt cgtcgtctac gccaacactt ctgctgcggt aaaagcgcgg
    10621 gcagattggg tggtaacttc aagcattgcc gtcgaactta ttgatcatct ggatagtttg
    10681 ggtgaaaaaa tcatctgggc acccgacaaa catctggggc gttacgtgca aaaaaagacg
    10741 ggcgcagaca ttttatgctg gcagggtgcc tgcattgtgc atgatgagtt taagactcag
    10801 gcgttaaccc gcttgcaaga agaatacccg gatgctgcca tactggtgca tccagaatca
    10861 ccacaagcta ttgtcgatat ggcggatgcg gtcggttcca ccagtcaact gatcgctgct
    10921 gcgaaaacat tgccacatca gaggcttatt gtggcaaccg atcggggtat tttctacaaa
    10981 atgcagcagg cggtgccaga taaagagtta ctggaagcac caaccgcggg tgagggcgca
    11041 acctgccgca gctgcgcgca ttgtccgtgg atggccatga atggcctcca ggccatcgct
    11101 gaggcattag aacaggaagg aagcaattac gaggttcatg ttgatgaaag gctgcgagag
    11161 agggcgctgg tgccgctcaa tcgtatgctg gattttgcgg ctacactacg tggataacga
    11221 ataataaggc gtaacgttac gctttggggg aaagatggat ttttttagtg tgcagaatat
    11281 cctggtacat ataccaatag gagcaggcgg ttatgatctc tcatggatcg aagcggtagg
    11341 cacaatcgcc gggttgctgt gtattggcct tgccagtctg gagaagatca gcaactactt
    11401 ctttggcctg atcaacgtca ccttgtttgg cattattttc tttcagattc agctgtatgc
    11461 cagcctgcta ttacaggtgt ttttctttgc cgcgaatatt tacggctggt atgcgtggtc
    11521 gcgacaaacc agtcagaacg aggcggagtt aaaaattcgc tggttgccat tgccgaaggc
    11581 actcagctgg ttggcggttt gcgttgtttc gattggtctg atgacggtat ttatcaatcc
    11641 ggtgtttgca tttttgaccc gcgtggcagt catcatcatg caagcattag gattacaggt
    11701 tgcgatgcct gaactacaac cggacgcttt cccgttctgg gattcttgca tgatggtgtt
    11761 atctatcgtg gcaatgattc tgatgactcg taagtatgtg gaaaactggc tgttgtgggt
    11821 gattattaac gtgattagcg tcgttatttt tgcacttcag ggcgtttacg ccatgtctct
    11881 ggagtacatc atcctgacct ttattgcgct caacggcagc cggatgtgga tcaatagcgc
    11941 acgcgaaaga ggctcacgcg cgctgtccca ttaatggtga tgatgtgaat ggccagacac
    12001 cccttcattc aggtggcagt ctggcccatg acaaggttga tattccatct gaatggtggc
    12061 gtgctcaatc tgatagtgat ccatcaggta gtgttggatc tgatccaaca aggcatcgtg
    12121 atcgtgtggc gggatcacct gcacatgcaa cgtcatcacc ggcttctcgc ctaccatcca
    12181 tacatgcaca tggtgcacat tgcggacttc cgggatttcc cggcacatac gacgcttcag
    12241 ttcagcgata tccagcgata ccggtgcacc ctcaagtaat tcattcacgc tatctttcaa
    12301 cagtcgccac gcgctacgca gaaccagaag cgacaccagt atcgagagaa tggggtcagc
    12361 aggtgtccag ccggtccaga taataatcaa cgcggcaata atagccccaa ccgaacccag
    12421 caggtccccg agcacatgca gtgccgctgc tcgtacgttg aggtttttct cttcgctgcc
    12481 gtgatgaagt aaccagaaag aaagtatatt tgccagcaac ccagccacgg caattgccat
    12541 catcatgccg ccctcgaccg gacgcggcgt gcggaagcgt tctatcgcct cccagacaat
    12601 caaaatggta attaccacga gggcgatggc attcacaaac gccgcgagcg tggtcaatct
    12661 tagccaaccg aaagtgtggc gaatggtggg aggacgacgt gaaaattgca cggcgagtag
    12721 ggcgaaaagc agggcggcag tatcggtcaa catatgaccc gcatcggcca gcaaagccag
    12781 agaaccggaa agaaaaccac caatgacttc taccagcata aacccggcag tcacgccgaa
    12841 agcatacaac aagcgacgag cattattatc ttctggcagg tgtgaggacg tgtgtgagtg
    12901 tgagtgcgcc atgacgagtc atcccttaat tattctcact taactttatg acatcatact
    12961 gcttttagaa gggaaaaatt aaaagggaga gactccgctc tcccattatt ggctattttg
    13021 cagggttact gcgtggtacc gtcggttttg gtatcgacat cattattgat gccatcaccg
    13081 gtttgtacct ttttattgat atccgggcag cgaccatctt tgcacatggt gttcttgtgc
    13141 tcttcatctt tggtcatgcc gtcattgttc attgaagaac catccggatg cagcatagtg
    13201 ccgccagaac cggtatttac cccgttattg tcgacgttat ttggcgcgac attttcacgg
    13261 gcgtcagggg ctacctggcc cgcatcagct gcggcgtttg cctggccgtt attggtttgc
    13321 gctccgctat cggcagccag tgcggcaccg ctggcaaggc ttagagtggc agtcagaaat
    13381 agtgtggcca gttttgtcat tttcatagga tgctcctgtt atggtcgtta tgtcggataa
    13441 cctcttccaa cagtgcattt gcaggtgaat ataaggcatt ggtttaagat ttcagccagg
    13501 caaagaaatc cagcagagaa tcttgaaata attaacaaac aaaggagtta cagttagaaa
    13561 ttgtaggaga gatctcgttt ttcgcgacaa tctggcgttt ttcttgctaa ttccaggatt
    13621 aatccgttca tagtgtaaaa ccccgtttac acattctgac ggaagatata gattggaagt
    13681 attgcattca ctaagataag tatggcaaca ctggaacaga catgaattat cagaacgacg
    13741 atttacgcat caaagaaatc aaagagttac ttcctcctgt cgcattgctg gaaaaattcc
    13801 ccgctactga aaatgccgcg aatacggttg cccatgcccg aaaagcgatc cataagatcc
    13861 tgaaaggtaa tgatgatcgc ctgttggtgg tgattggccc gtgctcaatt catgatcctg
    13921 tcgcggctaa agagtatgcc actcgcttgc tggcgctgcg tgaagagctg aaagatgagc
    13981 tggaaatcgt aatgcgcgtc tattttgaaa agccgcgtac tacggtgggc tggaaagggc
    14041 tgattaacga tccgcatatg gataacagct tccagatcaa cgacggtctg cgtatagccc
    14101 gtaaattgct gcttgatatt aacgacagcg gtctgccagc ggcgggtgaa ttcctcgata
    14161 tgatcactcc tcagtatctc gctgacctga tgagctgggg cgcaattggt gcacgtacca
    14221 cggaatcgca ggtgcaccgc gaactggcgt ctggtctttc ttgtccggta ggttttaaaa
    14281 atggcacaga cggtacgatt aaagtggcta tcgatgccat taatgccgcc ggtgcgccgc
    14341 actgcttcct gtccgtaact aaatgggggc attcggcgat tgtgaatacc agcggtaacg
    14401 gcgattgcca tatcattctg cgcggcggta aagagcctaa ctacagcgcg aagcacgttg
    14461 ctgaagtgaa agaagggctg aacaaagcag gtctgccagc tcaggtgatg atcgatttca
    14521 gccatgctaa ttcgtccaaa caattcaaaa agcagatgga tgtttgtgct gacgtttgcc
    14581 agcagattgc cggtggcgaa aaggccatta tcggtgtgat ggtggaaagt catctggtgg
    14641 aaggcaatca gagcctggag agcggggagc cgctggccta tggtaagagc atcaccgatg
    14701 cctgcattgg ctgggaagat accgatgctc tgttacgtca actggcgaac gcagtgaaag
    14761 cgcgtcgcgg gtaaagttta attgtcggat gcgccgtcag agtggcgtat ccgatgaatc
    14821 accgtagccc tgacaagacg cgcagcgtcg catcaggcaa tgtgctccat tgttagcgac
    14881 gacaaacaaa aaagccgact cacttgcagt cggctttctc atttttaaac gaatgacgtt
    14941 tacttcgctt taccctggtt cgcaacggct gctgctttcg ccgcgatctc gtcagcatta
    15001 cccagataat agcgtttcag cggtttgaag ttctcgtcaa actcataaac cagcggcacg
    15061 ccggtcggga tattcagctc aagaatctct tcttcgctca tgttatcgag atatttcacc
    15121 agcgcacgta acgagttacc gtgtgcagcg atgattacgc gctcaccgct cttcatacgc
    15181 ggcagaatgg tttcattcca gtaagggatc acgcggtcaa tggtcagcgc caggctttcc
    15241 gtcagcggca gttctttctc gctcagtttc gcgtaacgcg gatcgtgacc cggataacgc
    15301 tcatcatctt tagtcagttc cggcggagtc actgcaaaac cacgacgcca ctgtttcacc
    15361 tgctcgtcgc catatttttc agcagtttcc gctttgttca gaccctgcaa cgcaccgtag
    15421 tgacgttcgt tcagtttcca ggatttctca acgggcagcc atgcctgatc cagttcgtcc
    15481 agcacattcc acagggtatg gatagcgcgt ttcagcacag aggtgtaagc aaagtcaaag
    15541 ctgtaacctt cctctttcag cagcttacct gctgcttttg cttcgcttac gcctttctca
    15601 gacagatcca cgtcgtacca accggtgaaa cggttttctt tgttccactg actttcgcca
    15661 tgacgaacca gaaccagctt agttacagcc atatacttac tcctcaaatc atcttttaat
    15721 gataataatt ctcattatat tgccgtgacg aagcaacagc aatgcttacg cataaccata
    15781 gcgaaaatag tggcgcagtg taaggttgtt gtgaatattg agttgcaaat atgtcggtgt
    15841 ttgctggtga tttgaacaat atgagataaa gccctcatga cgagggctta acattactca
    15901 gcaataaact gatattccgt caggctggaa tactcttcac caggatgcag gaaacagtcc
    15961 ggttgcggcc attcagggtg gttcgggctg tccggcagaa actcgctttc caacgccagc
    16021 ccttgccagt cggcgtaagg ttcggttccc cgcgacggtg tgccgccgag gtagttgccg
    16081 gagtagaatt gcagagccgg agcggtggtg tagaccttca gctgcaattt ttcatctgct
    16141 gaccagacat gcgccgccac tttcttgcca tcgcctttgg cctgtaacaa gaatgcgtga
    16201 tcgtaacctt tcactttgcg ctgatcgtcg tcggcaagaa actcactggc gatgattttg
    16261 gcgctgcgga aatcaaaaga cgttccggcg acagatttca ggccgtcgtg cggaataccg
    16321 ccttcatcaa ccggcagata ttcgtccgcc agaatctgca acttgtgatt gcgcacgtca
    16381 gactgcttgc cgtcaagatt gaaatagacg tgattagtca tattcaccgg gcaaggttta
    16441 tcaactgtgg cgcgataagt aatggagata cggttatcgt cggtcagacg atattgcacc
    16501 gtcgcgccga gattacccgg gaagccctga tcgccatcat ctgaactcag ggcaaacagc
    16561 acctgacgat cgttctggtt cacaatctgc cagcgacgtt tgtcgaaccc ttccgggccg
    16621 ccgtgcagct ggttaacgcc ctgacttggc gaaagcgtca cggtttcacc gtcaaaggta
    16681 taacgactat tggcgatgcg gttggcataa cgaccaatag aggcacccag aaacgcggcc
    16741 tgatcctgat agcattccgg gctggcacag ccgagcagcg cctcgcggac gctgccatcg
    16801 gaaagcggaa tacgggctga aagtaaagtc gcaccccagt ccatcagcgt gactaccatc
    16861 cctgcgttgt tacgcaaagt taacagtcgg tacggctgac catcgggtgc cagtgcggga
    16921 gtttcgttca gcactgtcct gctccttgtg atggtttaca aacgtaaaaa gtctctttaa
    16981 tacctgtttt tgcttcatat tgttctgcga cagcttgctg tacggcaggc accagctctt
    17041 ccgggatcag cgcgacgata cagccgccaa atccgccgcc ggtcatgcgt acgccacctt
    17101 tgtcgccaat cacagctttg acgatttcta ccagagtgtc aatttgcggc acggtgattt
    17161 cgaaatcatc gcgcatagag gcatgagact ccgccatcaa ctcgcccata cgtttcaggt
    17221 cgccttgctc cagcgcgctg gcagcttcaa cggtgcgggc gttttcagtc aggatatgac
    17281 gcacgcgttt cgccacgatt gggtccagct catgtgcaac agcgttgaac tcttcaatgg
    17341 tgacatcgcg cagagctggc tgctggaaga aacgcgcacc ggtttcgcac tgttcacgac
    17401 gggtgttgta ttcgctgcca accagggtac gtttgaagtt actgttgatg atgacgacag
    17461 ccacaccttt cggcatggaa actgctttgg tccccagtga gcgacagtca atcagcaagg
    17521 catgatcttt cttgccgagt gcggaaatta gctgatccat gatcccgcag ttacagccaa
    17581 caaactggtt ttctgcttcc tgaccgttaa gcgcgatttg tgcgccgtcc agcggcagat
    17641 gataaagctg ctgcaatacg gttccgaccg cgacttccag tgaagcggaa gaacttaacc
    17701 cggcaccctg cggcacattg ccgctgatca ccatgtccac accgccgaag ctgttgttac
    17761 gcagttgcag atgtttcacc acgccacgaa cgtagttcgc ccattgatag ttttcatgcg
    17821 cgacaatggg cgcatcaagg gaaaactcat caagctgatt ttcataatcg gctgccatta
    17881 cgcgaacttt acggtcatcg cgtggcgcac agctgatcac ggtttgataa tcaatcgcgc
    17941 agggcagaac gaaaccgtcg ttgtagtcgg tgtgttcacc aatcaaattc acgcggccag
    18001 gcgcctgaat ggtatgagtg gcagggtagc caaatgcgtt ggcaaacaga gattgtgttt
    18061 tttctttcag actcatttct tacactccgg attcgcgaaa atggatatcg ctgactgcgc
    18121 gcaaacgctc tgctgcctgt tctgcggtca ggtctcgctg ggtttctgcc agcatttcat
    18181 aaccaaccat aaatttacgt acggtggcgg agcgcaacag aggcggataa aagtgcgcgt
    18241 gcagctgcca gtgttgattc tcttcgccat taaacggtgc gccgtgccag cccatagagt
    18301 aggggaagga gcactggaag aggttgtcat aacgactggt cagctttttc aacgccagtg
    18361 ccaaatcgct gcgctgggcg tcggtcaaat cggtgatccg caaaacgtgg gctttgggca
    18421 gaagtagcgt ttcgaacggc caggcagccc agtaaggcac aactgctaac cagtgttcgg
    18481 tttcgacaac ggtacggcta ccgtctgcca gctcgcgctg aacataatcc accagcattg
    18541 gcgatttctg ctcggcaaaa tattcttttt gcaggcggtc ttcgcgctca gcttcgttag
    18601 gcaggaagct atttgcccaa atctgtccgt gcggatgcgg gttagagcag cccatcgccg
    18661 cacctttgtt ttcaaagacc tgcacccacg ggtatgtttt ccccagttct gcggtttgct
    18721 cctgccaggt tttgacgatt tccgtcaatg ccgcaacgct cagttctggc agcgttttac
    18781 tgtgatccgg tgaaaagcag atcacccggc tggtaccgcg cgcgctctgg caacgcatca
    18841 aaggatcgtt gctttctggc gcatctggcg tgtcagacat caaggccgca aagtcattag
    18901 tgaaaacgta agtcccggtg taatcggggt ttttatcgcc tgtcacccgc acattacctg
    18961 cgcagaggaa gcaatctgga tcgtgcgcag gtaacacctg tttggctggc gtttcctgcg
    19021 ccccctgcca ggggcgctta gcgcggtgcg gtgaaaccag aatccattgc ccggtgagct
    19081 ggttgtagcg gcgatgtgga tgatcaacgg gattaaattg cgtcatggtc gttccttaat
    19141 cgggatatcc ctgtggatgg cgtgactgcc agtgccaggt gtcctgcgcc atttcatcga
    19201 gtgtgcgcgt tacgcgccag ttcagttcac ggtcggcttt gctggcgtcc gcccagtagg
    19261 ccggaaggtc gccctcgcga cgcggtgcaa aatgatagtt aaccggtttg ccgcaggctt
    19321 tgctgaaggc attaaccacg tccagcacgc tgctgcctac gccagcaccg aggttgtaga
    19381 tgtgtacgcc tggcttgttc gccagttttt ccatcgccac gacgtgaccg tccgccagat
    19441 ccattacgtg gatgtaatcg cgtacgccag taccgtcttc ggtcggataa tcgttaccaa
    19501 aaatcgccag cgagtcgcga cggcctacag caacctgggc gatgtatggc ataaggttat
    19561 tcggaatgcc ttgcggatct tcgcccatat cgcccgacgg atgtgcgcca accgggttga
    19621 agtagcgcag cagggcaatg ctccagtccg gctgggcttt ttgcagatcg gtgaggatct
    19681 gttccaccat cagcttgctt ttgccgtaag ggctttgcgg tgtgccggtc gggaagcttt
    19741 caacgtatgg aattttgggc tgatcgccat aaacggtggc ggaggagcta aaaataaagt
    19801 ttttgacgtt agcggcgcgc atggcgctaa tcaggcgcag agtaccgttg acattgttgt
    19861 cgtaatattc cagcggtttt tgtaccgatt cgccaacggc tttcagcccg gcgaagtgga
    19921 tcacggtgtc gatagcgtga tcgtgcagga tctcggtcat caacgcttcg ttacggatat
    19981 cgccttcaac aaacgtcgga tgtttgccgc ctaaacgctc gataacaggc agtacgctgc
    20041 gcttactgtt acagaggtta tcaagaatga tgacatcatg accgttttgc agtaattgca
    20101 cacaggtatg acttccaatg taaccgctac caccagtgac cagaactctc ataattcgct
    20161 ccattaggct tatggtatga aataaccata gcataacaaa gatgcgaaaa gtgtgacatg
    20221 gaataaatta gtggaatcgt ttacacaaga atttagccgt tttttatgcg cgattaagtg
    20281 attataaaac agagggattg tgaatgattg cgctttttat ctgaaaaaaa gacgcagttt
    20341 catgcctgga tgcgtctgac cgtgtgccgg ataaagcgtt tacgccgcat ccggcacagt
    20401 cagcattacc gacttagttt atttttgtca gcgcataatg atagaggcca ccgtccggca
    20461 cgaactccag acgatgggta atacaagccg gcgcatcttc agcatggtgc gaaacaaaca
    20521 acaattgcgt ttcaccttcg ctaatcagca catcaacaaa acggcgaata agctggcgat
    20581 tgagcggatc aagcccctgc aatggttcgt cgagaataag caacgtcgga tgtttcacca
    20641 gtgcgcggac gatcagcgcc agacgctgct gtccccagga aagactatgg aacggagcgt
    20701 cagccgtgcg tttatcaatg ccgagaatat ccagccactg ctgcaccagt ttttgctggc
    20761 gatccgaaac ggcctgataa atgccaatcg aatcaaaata gccagaaaga atcacattac
    20821 gcacagtagt actgacccgg taatccagat gcaaactact gctgacgtaa ccgatatgct
    20881 ttttgatatc ccagatggtt tcgccgctgc cacgacgtcg tccgaaaagc gtcaaatcgt
    20941 tgctgtaacc ttgcggatga tcgccagtaa tcaggcttaa taacgtcgat tttcccgcgc
    21001 catttggccc gacaatttgc cagtgttcac ctggattcac ctgccagcta aggttattaa
    21061 gaatggggcg atcgttataa gaaaccacgc cattattcag cacaatgcgc ggttcgttgg
    21121 cgggtaaggc gtgacgtgct gaaggttcat ccggctccgg cagttgcaca ccttcaagct
    21181 gttcactatg cgccagttgc gcgacgagtg cttgttggag cagttcctct ttagcgccag
    21241 tttccgctaa cgtgcaatcc gccagcacgc cagcaaactg gacaaactcc gggatctcat
    21301 cgaagcgatt gagcaccagt accagagtaa taccggactg atgtaacgag gcgagtcgct
    21361 cagccagctg ctgacgtgag gcaacatcca ggccatcgaa cggctcatca agaatcaaca
    21421 agtcaggctc cgacatcagc gcctgacaca gcagggtttt tcgcgtctcg ccagtggaaa
    21481 ggtatttaaa gcgtcggtcg aggagggggg taataccgaa ctgctgtgcc agttgcatgc
    21541 agcgcggtgc atcctttact tcatcctgaa taatctcagc cgtagtgcgt ccggtgtcat
    21601 cttcgccagg gccgagcata tcggtgttat tccgctgcca ttcgtcgctg acgagctttt
    21661 gcaattgctc gaaggagaga cgagtgatgt gggaaaactg gctttgccgt tcacctttca
    21721 aaagcggaag ttcccccgcc agcgcgcggg ccagggccga tttcccgctt ccattcgaac
    21781 cgacaaacgc ccaactatca cccgcgttta acgttagctg aggcaattgc agcgtttttg
    21841 tgtcgctaag acgaaacgtg ccttgcaaaa tttgcaacga tgacatttta tatcccattt
    21901 tgtgcagcga ttactgacag ggatacgtgt ttcataacaa attgtcaaca cgcttagcac
    21961 agtgtggcga taatcacgct gtcggcatta aagtaggccg tgacattctg tccttgctga
    22021 agagaagtcg cttcatttac cggcactgtg gcgcacagtg tttgcccgtc cggtagcgcc
    22081 attaatactt cgcactgctc tgcgccgcgc tcaatatgac taataatacc cggtaattgg
    22141 ttgtcagcgt tttgcgcgac cgcttcgtcc tgagtaatgc ctacccacgg cgcttttagc
    22201 aatatcaaca cttctttgcc ttcatccagc cccagacgcg cgccgctttg tgcggtaatt
    22261 gcgactttca ggcgtgtttt tccatcagcc agtaagacat caacatgctg ttgaacgtca
    22321 tcatgatcgc gggcggtgat ggtaccgaac cactggttac gggcgctggt ttggagtgaa
    22381 aaacgtgaga tcgcggccag caggctgttc agcggcaggg cgtcatcgtc acttaacaca
    22441 tcaaaggctt tttgctggat ttgcgccagt aagtcataga gctgaatcag tcgctgacca
    22501 tagcgggtca gtactgcgcc gccgccacct ttaccgcctg ttgcgcgctc gaccagaata
    22561 tgctcactta actgattcat ctcgttaatg gcatcccagg cgcttttata gctgataccg
    22621 gcatctttcg ctccctggct aatggaaccg gaaagcgcaa tgtgttttag tagcgaaatg
    22681 cggcgcgggt cggcgaataa tttttgttgg agcttaaggg tgagaaggat ttcggcctgc
    22741 ataacaatgt cctggcaaaa gtcttattgt gacggaaaac gaacgccacg caaagctgac
    22801 cgcacaaaag gggagtgctt ttctgtgctt agcggttaga atagtctcat gactatatct
    22861 ggagttgacc atgttagagt tattaaaaag tctggtattc gccgtaatca tggtacctgt
    22921 cgtgatggcc atcatcctgg gtctgattta cggtcttggt gaagtattca acatcttttc
    22981 tggtgttggt aaaaaagacc agcccggaca aaatcattga ttccctgaat gcccgcttag
    23041 tcgggcattt tctttttctc aacttcctgc ttttcctgcc gatatttttt cttatctacc
    23101 tcacaaaggt tagcaataac tgctgggaaa attccgagtt agtcgttata ttgtcgccta
    23161 cataacgtta cattaagggg ttaccaatgg ctcgtaaatg gttgaacttg tttgctgggg
    23221 cggcactctc tttcgctgtt gctggcaatg cactggcaga tgaagggaaa atcacggtgt
    23281 tcgccgccgc atcactgact aacgcaatgc aggacattgc tacgcagtat aaaaaagaga
    23341 aaggcgtgga tgtggtttct tctttcgctt cgtcatctac tctcgcccgt cagattgaag
    23401 cgggtgcccc tgcggatctg tttatttctg ccgatcagaa atggatggat tatgcggttg
    23461 ataaaaaagc gatcgataca gctacgcgtc agacactgct cggcaatagc ctggtcgttg
    23521 tagcaccgaa agccagcgag cagaaagatt ttaccatcga cagcaaaacc aactggactt
    23581 cactgctgaa tggcggtcgc ctggcggttg gcgatccgga acatgttccc gctggcattt
    23641 atgcaaaaga agcactgcaa aaactgggcg catgggatac gctctccccg aaactggctc
    23701 cggcggaaga tgttcgtggc gcgctggcgt tggtcgaacg caatgaagcg ccgttgggca
    23761 ttgtttacgg ttctgacgca gttgctagca aaggggtaaa agtggttgcc accttcccgg
    23821 aagattcaca taaaaaagtg gaatatccgg ttgctgttgt ggaagggcat aacaatgcga
    23881 cagtgaaagc attttatgat tatctgaagg gaccgcaggc agcggaaatc tttaaacgtt
    23941 acggatttac aaccaagtaa tgatactgac cgatccagaa tggcaggcag ttttattaag
    24001 cctgaaagtt tcttccctgg ctgtgctgtt tagcctgccg tttgggatct tttttgcctg
    24061 gttactggtg cgttgcacgt ttccgggcaa agctctgctc gacagcgtac tgcatctacc
    24121 gctggtgtta ccgcccgtgg tcgtcggtta cttattatta gtttcgatgg ggcggcgcgg
    24181 atttatcggt gaacgtctgt atgactggtt tggtattacc ttcgccttta gctggcgcgg
    24241 cgcggttctc gctgccgccg tcatgtcgtt tccgctgatg gtgcgggcaa ttcgtctggc
    24301 gctggaaggg gttgatgtca aactggaaca ggccgcaaga acactggggg ccgggcgctg
    24361 gcgcgttttc tttactatca cgttaccgct gaccttaccg ggaattattg ttggtacggt
    24421 actggctttt gctcgttctc tcggtgaatt tggcgcaacc atcacctttg tgtcgaacat
    24481 tcctggtgaa acgcgaacca ttccttctgc catgtatacc ctgattcaga cccccggcgg
    24541 ggaaagtggc gcagcgagac tgtgcattat ttctattgcg ctggcgatga tctccctgtt
    24601 gatttcagaa tggctggcca gaatcagccg tgaacgggcg gggcgctaat catgctggaa
    24661 ctgaattttt cccagacgtt gggcaaccat tgcctgacta ttaatgaaac gctgcccgcc
    24721 aatggcatca ctgctatctt tggcgtctcc ggtgccggaa aaacttcgct gattaacgcc
    24781 atcagtggac tgacgcgccc gcaaaaaggg aggattgtcc tcaatggtcg ggtactaaat
    24841 gatgccgaaa aaggtatctg cctgacgccg gaaaagcgtc gcgttggcta tgtttttcag
    24901 gatgcgcggc tgttcccgca ttacaaagtg cgtggcaatc tgcgctacgg catggcgaaa
    24961 agcatggtcg atcagttcga taagctggtg gcgcttttag gcattgaacc gttgcttgac
    25021 cgtttaccag gcagcctgtc cggaggcgaa aaacagcgcg tagcgattgg gcgggctttg
    25081 ctgacggcac cagaattgct gttgctggat gaaccgctgg cgtcactgga tattccgcgt
    25141 aaacgcgaac tgttgcctta tctgcaacgt ctgacgcggg aaatcaacat cccgatgttg
    25201 tatgtcagcc attcgctgga tgagatcctc catctggcag acagagtgat ggtactggaa
    25261 aacggtcagg tgaaagcctt tggcgcgctg gaagaagtct ggggtagtag cgtgatgaat
    25321 ccgtggctgc cgaaagagca acaaagtagc attctgaaag tgacggtact tgagcatcat
    25381 ccgcattacg cgatgaccgc gctggcgctg ggcgatcagc atttgtgggt caataagctg
    25441 gacgaaccgc tgcaagctgc gctacgtatc cgcattcagg cttccgatgt ttctctggtt
    25501 ttacaaccgc cgcagcaaac cagcattcgt aacgtattgc gggcaaaagt tgttaatagt
    25561 tatgacgaca acggccaggt ggaagtggaa ctggaagtcg gcggtaaaac gctgtgggcg
    25621 cgtatcagcc cgtgggccag ggatgaactg gcgatcaaac ctggcctgtg gctgtacgcg
    25681 caaattaaaa gtgtgtcgat aaccgcctga ttaaatcagg tggctataaa tgaactgggc
    25741 aatgctgtcg gtggtgttat caccaatcac aatgttggcg cgcgctttta ccgcgtcatc
    25801 ggcgttgccc atcgccacgc ctgtaccagc ggcctccagc atactgatat cattaaagtt
    25861 atcgccgaac gccacgacgt tttccatcga ccagccttgc gcctcaaccc atttcgtcaa
    25921 acgtttacct ttgctgttgc cgccgcgtgc aatatcaacc tgatcgtgcc aggaccattc
    25981 acactccagt cccagttcat gttcgacatg cttaccaaaa tgctgcaatt gcggcaggtc
    26041 atcgtgcgtg agggcgaact tccatacggc gttaacttgt tgcgccgttt cagccagaga
    26101 agcgacttgt gtgaaagtcg gacgctgttc cggcggcagg gtttgcgccc agttagacgt
    26161 gcgaatgaca tgcccggtcg ggtgctcata aaccatctcg tcatcgacat acatcagacc
    26221 gtgaatgtgg tgttcattca gcatctcaat gagttgtagg gctttattaa cgggcattgg
    26281 gtccgcttcc agcacggttt ttgcatgata atcatacaaa taggtgccat tacagcaaat
    26341 agcaggtgta tccagcgcca gcgcctgata aaaaggatga atagcgacgt gatggcgacc
    26401 tgtgacgatg attaattgat aacctgcttc gcgagcgcgg gccagggctt ctatcgatga
    26461 aggtagcagg gtctttttcg gggtcaataa ggtgccgtct aagtcgagag caatcacgcg
    26521 tgtggtcatg gcgtattcca gattaaggtt aagaattttc ttctgcgcga atggtacacc
    26581 gataccacta tcggacaaaa ttctgcgttt taattcagca ttcaccgcca aaagcgacta
    26641 attttagctg ttacagtcag ttgctaaatg caaaggagca ttcatgaagc aaacagttta
    26701 tatcgccagc cctgagagcc agcaaattca cgtctggaac ctgaatcatg aaggcgcact
    26761 gacgctgaca caggttgtcg atgtgccggg gcaggtgcag ccgatggtgg tcagcccgga
    26821 caaacgttat ctctatgttg gtgttcgccc tgagtttcgc gtcctggcgt atcgtatcgc
    26881 cccggacgat ggcgcactga cctttgccgc agagtctgcg ctgccgggta gtccgacgca
    26941 tatttccacc gatcaccagg ggcagtttgt ctttgtaggt tcttacaatg cgggtaacgt
    27001 gagcgtaacg cgtctggaag atggcctgcc agttggcgtc gtcgatgtgg tcgaggggct
    27061 ggacggttgc cattccgcca atatctcacc ggacaaccgt acgctgtggg ttccggcatt
    27121 aaagcaggat cgcatttgcc tgtttacggt cagcgatgat ggtcatctgg tggcgcagga
    27181 ccctgcggaa gtgactaccg ttgaaggggc cggcccgcgt catatggtat tccatccaaa
    27241 cgaacaatat gcatattgcg tcaatgagtt aaacagctca gtggatgtct gggaactgaa
    27301 agatccgcac ggcaatatcg aatgtgtcca gacgctggat atgatgccgg aaaacttctc
    27361 cgacacccgt tgggcggctg atattcatat taccccggat ggtcgtcact tatatgcctg
    27421 tgaccgtacc gccagcctga ttactgtctt cagcgtttcg gaagatggca gcgtgttgag
    27481 taaagagggc ttccagccaa ctgaaaccca gccgcgcggc tttaatatcg atcaccgtgg
    27541 taagtatctg attgccgccg ggcaaaaatc gcaccacatc tcggtatacg aaattgttgg
    27601 tgagcagggg ctactgcatg aaaaaggccg ctacgcggtc gggcagggac caatgtgggt
    27661 ggtggttaac gcacactaac cgctgattta cccggcgcag tctctcctgc gccggtgtat
    27721 taacctatct cctgtaacgc atgtctctgg cgttcgacga tattggtcca caaattgtct
    27781 ttatcgtcag tccacaaatt aatcagcaag gcaaaaaagc gttctgctgc cggagaaagc
    27841 acggcatctt tgcggcgaat aatccccaat gtccgacgta taacgggttc caccagtggg
    27901 ataccaataa tagaagaata gggcgcatgg ggcattgcca gaccaggaag cgccgaaatt
    27961 cccagtcccg cctctaccag tcctaatgac gtcgaaagat gacgcacttc gtaaaaccag
    28021 tccagcttcc agggcttgtc ggctagctgt tgttctatca acaatcggtt gccactggag
    28081 gaacgtacgc caatcatttt gtaacccact aactcctgcc attctacaag ctgcttttta
    28141 gccaatggat ggtcacgccg acaggccagc acgaacggct cgttgaccag tggggtaaaa
    28201 tcaatggatg aatttgtgac gttgttcatg tttatgccaa agtctgattc gttacacagc
    28261 accgattcca tgcaattatt cgtgccttgt tccagaatcc gcactttaat attgggatac
    28321 agctcattaa atttaccgat tgccagcggt aaaaaataaa atactgcggt cggaatacac
    28381 gctaacgtca ccataccacg atgataagca ttcatatcac gaatattaaa aagcgtttca
    28441 tcgaatttct ttattaattc ccgcgcctct gggagcaacc tttttcctgc ttttgttaac
    28501 gtaaccttgc gagttgtacg ctcaaatagt tgaatatgta aatcttcctc catctttttt
    28561 attcttcgtg ttaatgcagg ttgcgtaata ttgagtaatt tagcggcatt gttaaatgaa
    28621 ctggactctg ccagtatgac aaatgccttc atacttgata attcatgctt cattattact
    28681 ccgggaaatg gaagcgacga ttttgggtgg ctggccgtta aaaattttaa ctgcatttag
    28741 ccaacttaaa ttaatgaaaa aatgttatta atcgttgagc taaagtcatt agagatgctt
    28801 tgcccttaat gtaaccatat cgcaataagt tatgttttta aattgagggc attattatga
    28861 aaaaaatacc ctgcgtgatg atgcgaggtg gaacctcgag gggcgcgttc ctgttagcgg
    28921 aacatttacc cgaagatcaa acgcagcgcg ataaaatatt gatggcaatt atgggttccg
    28981 gtaacgatct ggaaattgac ggtattggcg gcggtaatcc actgaccagt aaagtcgcca
    29041 ttattagccg ttccagcgat ccgcgtgctg atgttgatta tctgtttgct caagttatcg
    29101 tccatgagaa acgtgtcgat accacgccta actgcggcaa tatgctgtct ggcgttgggg
    29161 cattcgccat tgaaaatggt ttgattgcag cgacttcgcc agttacccgc gtacgtatcc
    29221 gcaacgtcaa tacgggtacg ttcatcgaag ctgatgtgca aacgccaaat ggtgttgtcg
    29281 agtacgaggg tagcgccaga attgacggcg taccgggtac tgccgcaccg gttgcgctca
    29341 ctttcctgaa tgccgctgga acgaaaaccg gaaaagtttt cccgactgat aatcagattg
    29401 attattttga cgatgtcccg gtgacctgta tcgatatggc gatgccagtc gtcattattc
    29461 cggctgaata tctgggaaaa acaggctatg aattaccggc ggaactggat gccgacaaag
    29521 cattattagc ccgcattgaa tctatccgtc tacaagcggg taaagcaatg ggcttaggcg
    29581 atgtcagtaa tatggttatc cctaaacctg tgcttatttc tccagcgcag aaaggcgggg
    29641 caattaatgt gcgttatttt atgccgcatt cttgccatcg cgcgctggcg ataaccggtg
    29701 ctattgctat ttccagtagt tgtgcattgg aaggtaccgt cacccgacaa atcgtccctt
    29761 ctgtaggata cggcaatatc aatattgaac accccagtgg tgggctcgac gttcatttaa
    29821 gtaatgaagg tcaggatgcc acgacgttac gcgcatctgt tattcggaca accagaaaaa
    29881 tattttccgg tgaagtttat cttccctgaa aagattcgtt gtcaggataa ggacaatcaa
    29941 taaaggactt ctgtatgagt catacagaaa gaacaggatt ttaaatgaat aagaaatcgt
    30001 tatggaagct aattctgata ttagcgatcc catgtattat tggctttatg ccagctccgg
    30061 caggattaag cgaactggcg tgggtgcttt ttggtattta cctggcggcc attgtggggc
    30121 tggttatcaa gcctttcccg gaacctgtcg tactgttaat tgccgttgct gcctccatgg
    30181 tggtggtcgg taacttatcc gacggtgcgt ttaagaccac cgccgtatta agcggttact
    30241 cttcaggtac tacctggctg gtgttctcgg cgtttacctt aagcgccgca tttgtgacca
    30301 ccggtttagg taaacgtatt gcctatctgc tgattggtaa aatcggtaac accacgctgg
    30361 gtctgggtta cgttacggta ttcctcgatc tggtattggc tccggcaaca ccgtctaaca
    30421 ccgcgcgtgc gggcggcatt gtgttaccga tcatcaacag cgtggcggtg gctttggggt
    30481 ccgaaccgga aaaaagtccg cgtcgtgtcg gacattacct gatgatgtcc atttacatgg
    30541 tcaccaaaac caccagctat atgttcttta ccgcaatggc ggggaacatt ctggcgctga
    30601 aaatgatcaa cgacattctg cacctgcaaa ttagctgggg tggatgggcg ctggccgccg
    30661 gattgccggg catcattatg ctgctggtca ccccgctggt gatttacacc atgtatccac
    30721 cagaaattaa gaaggtggat aacaaaacca tcgctaaagc gggccttgcc gaactgggac
    30781 cgatgaaaat ccgcgaaaaa atgctgctcg gtgtctttgt gctggcgctg ctgggctgga
    30841 ttttcagtaa gtctctgggg gttgatgaat cttcagtggc tattgttgtt atggcgacca
    30901 tgctgctgct gggtatcgtt acctgggaag acgtggttaa aaataaaggc ggctggaata
    30961 ccttaatctg gtacggcggt attatcggct taagctcctt attatcgaaa gttaaatttt
    31021 tcgaatggtt agctgaagtc tttaaaaata acctggcatt tgatggtcac ggtaacgttg
    31081 ctttcttcgt tattattttc ctcagcatca tcgtgcgtta tttcttcgct tccggtagtg
    31141 cctatatcgt tgctatgtta ccggtatttg ccatgctggc gaacgtctcc ggcgcaccgt
    31201 taatgttaac cgcgctggca ctgttgttct ccaactccta tggcggcatg gttactcact
    31261 atggcggcgc ggcaggtccg gtcatctttg gcgtgggtta taacgatatt aaatcctggt
    31321 ggttggtcgg tgcggtactg acgatattaa ccttcctggt gcatatcacc ctcggcgtgt
    31381 ggtggtggaa tatgctgatc ggctggaaca tgctgtaaat atactcgtca tacttcaagt
    31441 tgcatgtgct gcgtctgcgt tcgctcaccc cagtcactta cttatgtaag ctcctgggga
    31501 ttcactcgct tgtcgccttc ctgcaactcg aattatttag agtatatcca tttattttct
    31561 tgctgcgcac ttcacggtgc gcagatatct ggagcatttg atgatcaagt tatctgaaaa
    31621 aggcgtgttt ctcgccagta ataacgaaat aattgccgaa gaacatttca ccggcgaaat
    31681 taaaaaagaa gaagcaaaaa aaggcactat tgcctggtct attctctctt ctcataatac
    31741 gtccggaaat atggataaac ttaaaattaa gtttgattca ttagcctctc acgatattac
    31801 ctttgttggt attgtacaga ccgctaaagc gtccggtatg gaacgtttcc cgctgccgta
    31861 tgtgctgatc aactgccata actcactctg cgccgtcggc ggcaccatta acggtgatga
    31921 ccatgttttt ggtttatcgg cagctcagcg ttatggcggt atttttgtgc ctccgcatat
    31981 tgcggtcatc catcaatata tgcgtgagat gatggcaggt ggcggcaaaa tgatcctcgg
    32041 gtcagacagt cacacccgtt acggtgcatt agggacaatg gcagtcggtg agggcggcgg
    32101 cgagttggta aaacaattgc ttaatgacac ctgggatatc gactatccgg gagttgttgc
    32161 ggtgcatctg accggaaaac cagcgccgta tgtggggccg caggatgtgg cgttggctat
    32221 catcggtgcc gtgttcaaaa acggctacgt caaaaacaaa gtgatggaat tcgtaggtcc
    32281 cggtgttgct gcgctctcta ccgatttccg taacagcgtt gacgttatga ccactgaaac
    32341 gacctgttta agttctgtct ggcaaaccga tgaagaagtc cataactggc tggcgctgca
    32401 cggtcgcggc caggattact gccagcttaa ccctcaaccg atggcgtact acgatggctg
    32461 catcagcgtt gatttaagcg ccatcaaacc aatgattgcg ctgccgttcc acccgagcaa
    32521 cgtgtatgaa atcgacacac tgaaccagaa cttgaccgac attctgcgtg agattgaaat
    32581 tgagtccgaa cgcgtggcgc acggtaaagc caaactctcg ctgctggaca aagtggaaaa
    32641 tggtcgcctg aaagtgcagc aggggattat cgcgggctgt tctggcggta actacgaaaa
    32701 cgtcatcgcg gcggcgaatg cactgcgcgg tcaatcctgt ggcaatgaca ccttctcgct
    32761 ggcggtttac ccgtcatcac agccggtgtt tatggatctc gccaaaaaag gtgtggtagc
    32821 agatttgatt ggcgcaggcg caatcatcag aaccgcgttc tgcggcccat gctttggcgc
    32881 gggcgatacg ccaatcaaca acggtttgag tattcgccac accacgcgta acttcccgaa
    32941 ccgcgaaggc tctaagccag ctaatgggca gatgtcagcg gtggcgttga tggacgctcg
    33001 ttctatcgct gcgactgcgg caaacggtgg ctatttaacc tctgccagcg aactcgattg
    33061 ctgggacaac gtgccggagt acgccttcga tgtaacgccg tataaaaatc gcgtctatca
    33121 aggctttgtg aaaggggcgg cccagcaacc gctgatttac ggaccgaaca ttaaagactg
    33181 gccggaattg ggtgcgctga ctgacaatat cgtcctcaaa gtgtgctcga agatcctcga
    33241 cgaagtgacc accaccgacg aattgattcc ttccggtgaa acctcttctt atcgttcaaa
    33301 tccgattggt ctggcggagt ttaccctgtc acgccgcgat ccaggttatg ttggcagaag
    33361 taaagcgact gctgagctgg aaaatcagcg tctggcgggg aatgtcagcg agctgacaga
    33421 ggtgtttgcg cgcattaagc agattgctgg tcaggagcat attgatccgc tgcaaactga
    33481 aattggcagc atggtctatg cggtgaaacc aggcgatggt tctgcgcgtg aacaggcggc
    33541 gagttgtcag cgtgtgattg gcggtctggc gaatattgcc gaggagtacg cgactaaacg
    33601 ctatcgttct aacgtcatca actgggggat gttaccgctg cagatggtgg aagcgccaac
    33661 ctttgaagtg ggggattaca tttacatccc tggcattaaa gcggcgctgg ataatccggg
    33721 tacgacgttt aaaggttatg tgatccatga agatgcgccg gtaacggaaa ttacgctcta
    33781 tatggaaagt ctgactgctg aagagcgcga gattatcaag gcgggtagtt tgattaactt
    33841 caataaaaac cgtcagatgt aaaaagcgcc atgtgaatgt aggtcgcatt cggcacttat
    33901 tgtcggatgc gatgctggcg catcttatcc gacctacaaa tcgcatcgaa ccgtaggccg
    33961 gataaggcgt ttacgccgca tccggcaaat agttaatagc tcttacttct tcgcctctgc
    34021 aaccactttg ctacccacgc cgcggttatt gtattcccac atgcggttgt agttagtgtc
    34081 attcagattg cgctgtattt cgtcgctatc gtcaacgctg ccggtgttac ccgcaaacgg
    34141 acgattagag atcaccgcat cgccccacgg tttagccgtg ttaaaacctt cgttgatggc
    34201 gctatcacgg atcaccacct gaccgttggt attggcatca acatccagcg agcggcccag
    34261 ttgtgccacg ccatcaccgg aagcattgaa acggctgttt acggcgagga aaccgtagta
    34321 aatgttggac agcgtagccg gtgcaaacac atacgcttct tgctgagtac gtgagttcac
    34381 cacgcggaat tcggtgttat cgaacaccac tgcgccgcga ccagaaacga tatccacatc
    34441 cccttcaatg tagctgttgg tcaccagcgt acgcggctga cgattcgttt ccagacggtt
    34501 ctgcacaccg ctgttggtga caaagaaggt gttctgacga ccgagaatgt taacgttgtt
    34561 aatctgtacc tggtcaccgt cagtacgcag tgccaccgcc ggatggttac ccgcatctac
    34621 gctatcaccc agcgtgtttt cgatggtcag gttttgcagt tgcaggccat tgttttgtga
    34681 ccagaagacc gcagaacaca gaacaccgat actgtcgctg cgtttgctct ggcagctatc
    34741 gtacatatac cacgctggtt tacctggcat atatttgccg cgcgggttga cgtcgtgacg
    34801 ccagtcggca gggctcatgc ccccatcaag ggaaagccca atcttcacat caatcggttt
    34861 ttcacctgta ccgtacagag taattccacc tggagcagca gggacataca ccgttccctg
    34921 atactcacca ggcatcacgg caatatactg gcgcttgttg gtgcgcttga taattgccgc
    34981 atctaccgcc gcctgaatcg tggtatgcgt tacaccttga gtacccgccg ggccgacaac
    35041 aaagtcaggt tgcgcaggca gggttatcgg ggacggattc cacgctgcag cacctggtgt
    35101 cagggatgca aaatagtgtt gagcatcgaa attctgcgct tcttttgccg acagaatcgg
    35161 gcgagaagag gtaccaggcg cagtttgatc agaaggacgt tgatcgggtg gtgttgagct
    35221 acaggcggtc agcgtcacgc caaaagccaa tgccagcgcc agacgggaaa ctgaaaatgt
    35281 gttcacaggt tgctccgggc tatgaaatag aaaaatgaat ccgttgaagt ctgctttttt
    35341 atactaagtt ggcattataa aaaagcattg cttatcaatt tgttgcaacg aacaggtcac
    35401 tatcagtcaa aataaaatca ttatttgatt tcaattttgt cccactccct gcctctgtca
    35461 tcacgatact gtgatgccat ggtgtccgac ttatgcccga gaagatgttg agcaaactta
    35521 tcgcttatct gcttctcata gagtcttgca gacaaactgc gcaactcgtg aaaggtaggc
    35581 ggatcccctt cgaaggaaag acctgatgct tttcgtgcgc gcataaaata ccttgatact
    35641 gtgccggatg aaagcggttc acgacgagta gatgcaatta tggtttctcc gccaagaatc
    35701 tctttgcatt tatcaagtgt ttccttcatt gatatcccga gagcatcaac atgcaatgtt
    35761 gtagggatgg caatttttac gcctgttttg ctttgttcga cataaagata tccatctacg
    35821 atatcagacc acttcatttc gcataaatca ccaactcgct gcccggtaac aacagccagt
    35881 tccattgcaa gtctgagcca acatggtgat gattctgctg cttgataaat tttcaggtat
    35941 tcgtcagccg taagtcttga tctccttacc tctgattttg ctgcgcgagt ggcagcgacc
    36001 gggtttgttg ttatatggcc ttcagctatt gcctctcgga atgcatcgct cagtgttgat
    36061 ctgattaact tggctgacgc cgccttgccc tcgtctatgt atccattgag cattgccgca
    36121 atttcttttg tggtgatgtc ttcaagtgga gcatcaggca gccccctcct tattgcttta
    36181 attttgctca tgtaatttat gagtgtcttc tgcttgattc ctctgctggc gaggattttt
    36241 tcgtagcgat caagccatga atgtaacgta acagaattat cactgttgat tctcgctgtc
    36301 agaggcttgt gtttgtgtcc tgaaaataac tcaatattgg cctgtattgc ttcagtgatt
    36361 gctatcctcc tgtctcggcc taaaccaaac tctttacccg tccttgggtc cctgtagcag
    36421 taatatccat tgtttcttat ataaaggtta gggggtaaat cccggcgctc atgacttcgc
    36481 cttcttccca tttctgatcc tcttcaaaag gctacctgtt actggtcgat ttaagtcaac
    36541 ctttaccgct gattcgtgga acagatactc tcttccatcc ttaaccggag gagggaatat
    36601 cctgcattcg cgcacccatc gacgaactgt ttcaaggctt cttgggcgtc gctggcgtgc
    36661 gttccactcc tgaagtgtca agtacatcgc aaagtctccg caattacacg caagaaaaag
    36721 ccgccatcag gcggcttggt gttctttcag ttcttcaatt cgaatattgg ttatgtctgc
    36781 atgtgctatc tgcgcccata tcatccagtg gtcatagcag tcattgatgt tctctgcttc
    36841 gataactctg ttgaatggtt ctccattcca ttcacctgtg actcggaagt gcatttatca
    36901 tctccataaa acaaaacccg ccgtagcgag ttcagataaa agaaatcctc gtcagtgcga
    36961 ggatgctgtt cattgctgct atacactttt ttgctctcaa cgtaagcggt agctcattct
    37021 gttgggttgg tgcagttgct tttaggaaat gctatttacc ccttaaacgt cggctgaaag
    37081 agctaaaatc catgcaaaaa atttacgcaa ttttgtgtat tattgtgcag taagtaatga
    37141 gctattttct gcgcaaaaaa tggatggtaa atttgtccgg ggcaggaaaa attttatggg
    37201 cgctaaacat gaaaaaagat tcgtatcctt atttgatttg catgacagtt ttaggcctga
    37261 tctttatttt ccttttcttc tggtggcggg cagatattta cagggtcacg tttcttaatc
    37321 agagtatatc ccactattac attctgttta gcatgggaat agcttttctg ttatctctgt
    37381 tttgggttaa gaaggggata gtaaaacaaa gcggctggaa gagtctgtca gcatacctta
    37441 aggtttatgc agggatgtgc atatttgctg gattttttct gattataccc cttacaacac
    37501 taacttattt tttgcctgga gagacatcgt cttatgttgc accgtatcgg tatacttccg
    37561 gtagttcaaa aagttgttct ggagctgagg tggatgaccc cgatctacat gagaatattc
    37621 gcatttgcta tccgtatggc aattatgagt acgataatat tatctatgtt gaaaagaaaa
    37681 ttaatacatt aggtgcggta gtaacatatg cacagaccgc gcgtgatgat actgaatgag
    37741 atagtatata gcgggcaagt tttagttaat ttatcgaggt aatataattt acctcgactc
    37801 gtttattctg gtattaatat ttcgctttac gatcgatttt tatctgatga tatcatgcgg
    37861 ttttcatata ctgacttact gtcttttctc cgttagcgat tttctcctgc tcagcgatga
    37921 ttttatcttt ggcttctagt taatttcgct cacttcgaac ctctctgttt actgataagt
    37981 tccagatcct cctggcaact tgcacaagtc cgacaaccct gaacggccag gcgtcttcgc
    38041 tcatctatgg gatcgccaca ctcacaacaa tgagtggcag atatagcctg gtggttcagg
    38101 cggcgcattt ttattgctgt gttgcgctgt aattcttcaa tttctgatgc tgaatcaatg
    38161 atgtctgcca tcttccatta atccctgaat tgttggttaa tacgcttgag ggtgaatgcg
    38221 aataataaaa aaggagcctg tagctccctg atgattttgc ttttcatgtt caccgttcct
    38281 taaagacgcc gtttaacatg ccgattgcca gacttaagtg agtcggtgtg aatcccatca
    38341 gcgttaccgt ttcgcggtgc ttcttcagta cgctacggca aatgtcatcg acgtttttat
    38401 ccggaaacgg ctgtctggct tttttgattt cagaattagc ctgacgggca atactgcgaa
    38461 gggcgttttc ttgctgaggt gtcattgaac aagtcccatg tcggcaagca taagcacaca
    38521 gaatatgaag cccgctgcca gaaaaatgca ttcagtggtt gtcataccag gtctctctca
    38581 tctgcttctg ctttcgccac catcatttcc agcttttgcg aaagggatgt ggctaacgta
    38641 tgaaattctt cgtctgtttc tactggtatt ggcacaaacc tgactccaat ttgagcaagg
    38701 ctatgtgcca tctcaatact cgttcttaac tcaacaggag atgctttgtg catatcgcct
    38761 cccgtttatt atttatctcc tcagccagcc gctgggcttt cagcggattt cggataacag
    38821 aaaggccggg aaatacccag cctcgcttcg taacggagta gacgaaagtg atcgtgccta
    38881 cgcggatatt atcgtgagga tgcttcatcg ccattgctcc ccaaatacaa aaccaatttc
    38941 agccagtgcc tcgtccattt tttcgatgaa ctccggcacc atctcgtcaa aacccgccat
    39001 gtacttttca tcccgctcaa ccacgacata atgcaggcct tcacgcttca tacgcgggtc
    39061 atagttggca aagtaccagg catcttttcg cgtcacccac atgctgtact gcacctgggc
    39121 catgtaagcc gactttatgg cctcgaaacc accgagcctg aacttcatga aatcccggga
    39181 ggtaaacggg catttcagct caaggccgtt gccgtcactg cataaaccat cgggagagca
    39241 ggcggtgcgc atactttcgt cgcgatagat gatcggggat tcagtaacat tcacgccgga
    39301 agtgaattca aacagggttc tggcgtcgtt ctcgtactgt tttccccagg ccagcgcctt
    39361 agcattaact tccggagcca caccggtgca aacctcagcc agcagggtgt ggaagtagga
    39421 cattttcatg tcaggccact tctttcctga gcggggcttt gctatcacgt tgtgaacttc
    39481 tgaagcggtg atgacgccga gccgtaattt gtgccatgca tcatccccct gttcgacagc
    39541 tctcacgtcg atcccggtac gctgcaggat aatgtccggt gtcatgctgc caccttctgc
    39601 tcagtggctt tctgtttcag gaatccaaga gctttcactg cttcggcctg tgtcagttct
    39661 gacgatgcgc gaatgtcgcg gcgaaatatc tgggaacaga gcggcaataa gtcgtcatcc
    39721 catgttttat ccagggcgat tagcagagtg ttaatctcct gcatggtttc atcgttaacc
    39781 ggagtgatgt cgcgttccgg ctggcgttct gcagtgtatg cagtattttc gacaatgcgc
    39841 tcggcttcat ccttgtcata gataccagca aatccgaagg ccagacgggc acactgaatc
    39901 atagctttat gccgtaacat ccgtttggga tgcgactgcc acggccccgt gatttctctg
    39961 ccttcgcggg ttttgaatgg ttcgcggcgg cattcatcca tccattcggt aacgcagatc
    40021 ggatgattac ggtccttgcg gtaaatccgg catgtacagg attcattgtc ctgctcaaag
    40081 tccatgccat caaactgctg gttttcattg atgatgcggg accagccatc aacgcccacc
    40141 accggaacga tgccgttctg cttatcaggg aaggcgtaaa tttctttcgt ccacggatta
    40201 aggccgtact ggttggcgac gatcaacaat gcgatgaact gcgcatcgct ggcatgaaag
    40261 cgcgatgggg agacagggcg gcatgagacg acagcgacga agtttcaccg acatcatctg
    40321 cgaaaactgc aaataccttc caacgaaacg ctccagaaat aaacgcaagc caatcccaaa
    40381 agaatctgac gtaaaaacct tcaactacac ggctcacctg tgggatatcc ggtggctaag
    40441 acattgtgcg aggaaaacaa ggtgattgac caaaatcgaa gttacgaaca agaaagcgtc
    40501 gagcgggctt taacgtgcgc taactgcggt cagaagctgc atgtgctgga agttcacgtg
    40561 tgtgagcact gctgcgcaga gctgatgagc gattcgaata gctcgatgca cgaggaaaaa
    40621 gatgatggct aaaccagcgc gaagacgatg taaaaacgat gaatgtcggg aatggtttca
    40681 ccctgcattc gctaatcagt ggtggtgctc tccagagtgt ggaaccaaga tagcactcga
    40741 acgacgaagc aaagaacgcg aaaaagcgga aaaggcagaa aaggcagcag agaagaaacg
    40801 acgacgagag gagcagaaac agaaagataa acttaagatt caaaaactcg ccttaaagcc
    40861 ccgcagttac tggattaaac aagcccaaca agccgta
//
LOCUS       DAZFAB010000032        39733 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZFAB010000032 DAZFAB010000000
VERSION     DAZFAB010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39733
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.032"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(138..515)
                     /locus_tag="ACHJWA_001996"
     CDS             complement(138..515)
                     /locus_tag="ACHJWA_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HFO4308597.1"
                     /translation="MNLDEEFYELQKLFAQKDLLTEKRRSSGGGFMEILLVKRQNMKI
                     KIYQEKGHQLPHIHIDYGKQAHAASYAIQSGDRIEGDLPKKYDSDVSSWLGRNRDKIL
                     EIWNALQAGAPYEPLIAELTGGV"
     gene            complement(637..969)
                     /locus_tag="ACHJWA_001997"
     CDS             complement(637..969)
                     /locus_tag="ACHJWA_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HFO4308598.1"
                     /translation="MRTVEILQYTLRKGSGAAFHAIMQEISVPLHQSHGIDVVSFGNS
                     LDDLDCYYLIRAFDSAKSMTAVLDAFYASADWRSGPREDIIGSIENCIKTVISLPSES
                     VKGLRMQS"
     gene            complement(1973..2440)
                     /locus_tag="ACHJWA_001998"
     CDS             complement(1973..2440)
                     /locus_tag="ACHJWA_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HFO4308599.1"
                     /translation="MIKDAIFSPCGKYRYSLSRVWDESKPYTLFIGLNPSYADAEKDD
                     RTLSRCISFAKSWGYGGVYMANLFAFVHTQRHEMMKASDPIGKDNDSHLIRLVSGAGL
                     VVAAWGNEGRHLKRSTTVRQLLPESTMCFVLNATGEPKHPLYMKNDSVLIPLG"
     gene            2633..3202
                     /locus_tag="ACHJWA_001999"
     CDS             2633..3202
                     /locus_tag="ACHJWA_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO4308600.1"
                     /translation="MAIFGYGRVSTSQQDTENQRMELEQAGWTFDFWFSDVVSGKVPA
                     VQRKAFFEMLSKIRDGETLVVAKLDRLGRDAIDVLQTVRTLADRNIKVIVHQLGTTDL
                     TSAAGKLLLSMLAAVAEMERDLLIERTNAGLLRAKAEGRKLGRPAKIAPEARGAILDK
                     RAAGVSVSALAREYGVSRATLAALLNKGY"
     gene            complement(3401..4717)
                     /locus_tag="ACHJWA_002000"
     CDS             complement(3401..4717)
                     /locus_tag="ACHJWA_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4308601.1"
                     /translation="MNDGVMLDSDVIAGLEWLASTQENKQHFYQRLAKAQQFYIATTQ
                     KAANFGKQFDPEWYGSDVVAGYFAQAKSLIDNRRSYEVTNASNIIPWVKQLGVCAKSL
                     DRITGARDRAIRMLKNTTVLPDTALLELVLAGNYASEGYEVEFIPEQKGIAKTPEFRC
                     RLGDGDYFFVECKRLQKGPYVKQEILQHHIRSHLAELHIKSKKMNVWTDVTYLCEVKD
                     APENYIISHLKNFKGVFYEWNDDYGKGTIRPANLNLIREDITENGSLLVNTKLARLIK
                     GSPLEDENYQVVAMGRPDERDSRFITKVKLASLITWRCINEKSFDARSRHVTKTLAEI
                     DNQLLDYGLGVGHIALDVDIQKDVADKRREKNYAAIVNFEQKSKIVRLNVHYLVPRID
                     ENSAWMVDETADDFFTHDLVKYVIPLTKIFPEAEVFENDQPGWHQN"
     gene            complement(4869..5642)
                     /locus_tag="ACHJWA_002001"
     CDS             complement(4869..5642)
                     /locus_tag="ACHJWA_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4308602.1"
                     /translation="MSKTRLSKPGFSAKSVLTIMLIAASSASYAAESIDQLATRIGMD
                     HFREAIKKGERSAFKTLPAKGYKNGLTGLLIKTNTYVNPEYSFADCDTDTVIISVQEH
                     KTLPGCRISLKLATKDADGSYHDNGIRVEYGIAIGSKKFIANNSAWAQHAISGDKVLE
                     GALHPDKTNNNKDQMNNNKDQMCMGMAQFTANVTEGRNSGMTKREYYQRLDNMNGKAD
                     VTQQMITLYKTFVDYVYEHPKQDAKISGTYIYTNCMNNF"
     gene            5765..6223
                     /locus_tag="ACHJWA_002002"
     CDS             5765..6223
                     /locus_tag="ACHJWA_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4308603.1"
                     /translation="MTLRKLFGQRVKELRIATGMSQEAFADRCGFARSYMSRIERGGS
                     NASLDAIEVLANALSVEPWRLLVSDSSEDSDPELLVPYAADGSCFHPGLASTRDGSFG
                     VGDKTAQKRFGTFSEALEYLRSMETAKWRRPNPSGNWGIVSAVRWDKLRK"
     gene            6939..7721
                     /locus_tag="ACHJWA_002003"
                     /pseudo
     CDS             6939..7721
                     /locus_tag="ACHJWA_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008815333.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            7841..8028
                     /locus_tag="ACHJWA_002004"
                     /pseudo
     CDS             7841..8028
                     /locus_tag="ACHJWA_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611456.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(8226..9077)
                     /locus_tag="ACHJWA_002005"
     CDS             complement(8226..9077)
                     /locus_tag="ACHJWA_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorothioated DNA-binding restriction
                     endonuclease"
                     /protein_id="HFO4308604.1"
                     /translation="MASSKSLQQAIANIKIWHKGEQRAPHKPLLLLYVLAGYLNGHPR
                     LFDYGTEIYEPLHSLLERFGPQRSQYRPDMPFWRLQGDGIWQLHNAERCSTAGSSRQP
                     PVKELNEYHVAGGFDEQHYALVTGNKKLINTLAQQILEAHFTESIQEEIADELGFDLQ
                     QTRKQRDPLFRKNVLRAYNYQCAICGFNMRHDDTTVALEAAHIKWKQHGGPCEIPNGL
                     ALCAIHHKAFDKGSIGLDENMRVLVSDAVNGGGIVERLFWDFDGKTIALPQVRKNYPY
                     EGFVGER"
     gene            complement(9151..9393)
                     /locus_tag="ACHJWA_002006"
                     /pseudo
     CDS             complement(9151..9393)
                     /locus_tag="ACHJWA_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid mutarotase"
     gene            complement(9618..10721)
                     /gene="zorE"
                     /locus_tag="ACHJWA_002007"
     CDS             complement(9618..10721)
                     /gene="zorE"
                     /locus_tag="ACHJWA_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II Zorya anti-phage system protein ZorE"
                     /protein_id="HFO4308605.1"
                     /translation="MKLSIDISELIQLGKKMLPEGVDFFLDESPIDFDPIDIELSTGK
                     EVSIEDLDPGSGLISYHGRQVLLYIRDHSGRYDAAIVDGEKGKRFHIAWCRTLDEMRH
                     KNRFERYHATNRIDGLFEIDDGSGRSQDVDLRVCMNCLERLNYKGSIDKQRKREIFKS
                     FSLNEFFSDYSTCFRHMPKGIYDKTNSGYVENWKEISKEIREKANYVCNDCGVNLSTA
                     KNLCHVHHKNGIKYDNHHENLLVLCKDCHRKQPLHEGIFVTQAEMAIIQRLRSQQGLL
                     KAESWNEIYDLTDPSVHGDINMMQHKGFQPPVPGLDLPNSEHEIIATVEAAWPGLKIA
                     VNLTPAEVEGWRIYTVGELVKEIQTGAFTPAKL"
     gene            complement(10725..11432)
                     /gene="zorB2"
                     /locus_tag="ACHJWA_002008"
     CDS             complement(10725..11432)
                     /gene="zorB2"
                     /locus_tag="ACHJWA_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II Zorya anti-phage system protein ZorB2"
                     /protein_id="HFO4308606.1"
                     /translation="MDKIIGKQLPKKDQDNEHWVSMSDLMAGLMMVFMFISIAYMHYV
                     RIEKEKIKEVAVAYENAQLQLYNALDIEFAKDLQDWDAEIDKQTLEVRFKSPDVLFGL
                     GSTELKPKFKLILDDFFPRYLKVLDNYQEHITEVRIEGHTSTDWTGTTNPDIAYFNNM
                     ALSQGRTRAVLQYVYDIKNIATHQQWVKSKFAAVGYSSAHPILDKTGKEDPNRSRRVT
                     FKVVTNAELQIRKIIQE"
     gene            complement(11439..13091)
                     /gene="zorA2"
                     /locus_tag="ACHJWA_002009"
     CDS             complement(11439..13091)
                     /gene="zorA2"
                     /locus_tag="ACHJWA_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II Zorya anti-phage system protein ZorA2"
                     /protein_id="HFO4308607.1"
                     /translation="MLAQLFEQLFQSIDSTLITNIFIWAVIFVFLSAWWCDKKNIHSK
                     FREYAPTLMGALGILGTFIGIIIGLLNFNTESIDTSIPVLLGGLKTAFITSIVGMFFA
                     ILFNGMDAFFFANKRSALAENNPESVTPEHIYHELKEQNQTLTKLVSGINGDSEGSLI
                     AQIKLLRTEISDSSQAQLANHTHFSNKLWEQLEQFADLMAKGATEQIIDALRQVIIDF
                     NQNLTEQFGENFKALDASVKKLVEWQGNYKTQIEQMSEQYQQSVESLVETKTAVAGIW
                     EECKEIPLAMSELREVLQVNQHQISELSRHLETFVAIRDKATTVLPEIQNKMAEVGEL
                     LKSGAANVSASLEQTSQQILLNADSMRVALDEGTEGFRQSVTQTQQAFASMAHDVSNS
                     SETLTSTLGETITEMKQSGEEFLKSLESHSKELHRNMEQNTTNVIDMFSKTGEKINHQ
                     LSSNADNMFDSIQTSFDKASAGLTSQVRESIEKFALSINEQLHAFEQATEREMNREMQ
                     SLGNALLSISKGFVGNYEKLIKDYQIVMGQLQALISANKHRG"
     gene            13303..16662
                     /locus_tag="ACHJWA_002010"
     CDS             13303..16662
                     /locus_tag="ACHJWA_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4851/ECs_5259 family protein"
                     /protein_id="HFO4308608.1"
                     /translation="MLVKTPVNPLLQWLNMFFSRRSLSGADGRALYAYRCTDTEYESL
                     AELLRTYAPRSYPRTIFISYSDVLFSIYAAEFIRRTHTVGHPKWDTILDSINWKVPYV
                     HRQKLVNDGIRYWKRKIRNLGQASGYLHTLACEGGLPIRMIENESGYLITYFRRIYQA
                     LRGQSSQYPAAKIAQELGDTIPVTMQNELVYEIAGEFCETLCRLLSEHPPHSSDPVSA
                     LRKLSPDWHLQLPLVLPEANAAEIVRRLLSQSSEIRSASSLQVERIWVDVDDSWYCDA
                     RFRFPATMRTEQLTSLFECHIQPEQTRLIISGKWKNGGARLAMLSRYEQQDWRVELLP
                     IAMQKLSGADAMAEISLSLHEGPILLGHTIPKGGYELTEELPWVFEAMNESESQLKLV
                     GMGSVSSRLNALFISLPKNSHLDISGEGEFDIPRLLKNSERSLTKISGVFSVVLHDGA
                     VCTIRTQQLYDSAIEYYIKSTEVELVKSDYPVHRAWPKIGWKKDLQYGIVPEKELFWR
                     SIRSGNNAWYSVASEMPKGQIEVRRIVNDEVLFSGKVVVLPADFDINIIPESAQQGII
                     MLSGITDTRIDKYSNNEKVTLKSDYSQNECAIYYNSSLMLENTVDLRVSWKDGSNLKL
                     LLPKPVSGGRFVTNDGSVHFDGVASIAHLHGIDAELLTISCAGRGYLNIELLDENPVA
                     EKFRYLHADLPLLSGRNDKLQQISLYENYNLLNAMLACAWNSNSTLCVDFYSDRFGKD
                     KATLNIKRYDGSFIEHDQGLLVDIKNSVVFPANRIDELVVDAISLKNPGLHISLLKKD
                     EFAYDLSALNVQDSPWLIVGKLDGTARIAPVIKWMLPVLQTNDLLLNALCEADPEQRK
                     KNFNELIFEIDNNPLQNYCCLLTEYIKKYKMNNGLSLLDLDLFRGISSNYRVVVQLLI
                     SSCLSGDSDTIYDIQEELPFSWGWIPVSIWKDVFQKCWTYLEKQINDKTLALHILQPF
                     IAFMNHRAHIDRRLAPIANMLLTYSESLPTGCDVLPTVSREQFNEAKQMLLRNPDSFG
                     RISIFPKELWSSAITPELKSVFNKLWIEDKYHSRLEKRFNLMLVAALLTQKDNNLIHQ
                     LSALFEFHYQQAPQQLGVIYQYYFEQAGVCH"
     gene            16662..22976
                     /locus_tag="ACHJWA_002011"
     CDS             16662..22976
                     /locus_tag="ACHJWA_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO4308609.1"
                     /translation="MDVQQLHYAPLLNQLHQRAVRSVVSQLALRSKSLTHYLESQYFQ
                     PPGQEGSLLADPVFESTFGWMPADETMEDLRDKLISSPLVDALSKAPVPFRHQLTAWR
                     TILEDKKSLLVSSGTGSGKTECFMVPVLEDLIRQQKSTRQSLTGTQAIFLYPLNALIN
                     SQQERLREWTRGFKGKIRFALYNGETRHTKYEIQEDQLKVPEQALSREAIYEAPPSIM
                     VTNTTMLEYMLIRRKDAPIIEKSQGMLKYIVLDEAHSYIGSQAAELALLLRRVMQAFN
                     VGPGTDKPVQIIATSATIGEDSPEGNKELAKFVADLAGVTERDVKVVRGYRQIPRVSE
                     SLIRHEYPTSLTSLKSLSPQDLYQQLCHYRVAQQLRQALTHPGRQAVRLSELLNVARR
                     TWPDINHRELLQLLDLMARSREGELAFTPLRMHGFIRTLAGLWACSNKQCSHKAHELN
                     QSDWPFGQVWFEQRQYCDCGAPVFEVLRCSGCGSAYLSAKEEMRGDGTNWLVAQPAAA
                     EVDEFALDVDVYSEDEDNDELDNNSQFDRLIASDGEKYIISLEETTAGKIDSEAQKHY
                     EINLLRPESRGEGRNNSFACVCCGDTQRKNNPLFRPLRLGAPFFLNEIIPTLLEFSPL
                     PQTREEQLGPFNGRRLLTFTDSRQGTARISARLQQDADKATLRALCYQELANITEPSA
                     KLSPAHCALLEKVISQFSVLPLKNSIDALKKAMAANEELSPTDIQALKIIEPMVSSLS
                     TEHAEAMQILQANSGNQQKKTMSWQTLSEQLVGSVDLADRMRGSFKELSGLDLTKQQF
                     ADFCLYSEFGRRPKNAWTLESLGLVAIHYPFIDKVTTCPQDWKTLLPDPDKQLAEWRN
                     LLKITLDFFIRENSAVFYENEQYPRWMGARFPVKMLQGPDKKNKGQKRDQLWPQIRDR
                     HYNRVIKLLIAVFPAIQPEQAHWKSLVNHLMIEVWDAIRPCLRQFESGYQLDIKQQAE
                     FYSPKQVWRCPYTRRALDVTLLGYSPYLPGSKEIAPEKAVLIEMPELPVRHWRLSGGG
                     EIAREERLEWLESNALIQHAREEGLWSTRSDRLALKDSWYRLEEHSAQRTPEQNQFNE
                     KQFKSGKVNVLNCSTTMEMGVDIGGMSLVAMNNVPPAPANYLQRAGRAGRRGESASAA
                     ITLCKNTAHGMEVFKDPLWAFNTTASAPRVRFGSSSIVQRHVNALVLGLFLRAEVPDA
                     TKLSCKWFFEGDESQCLRFLHWLNHQADQLADKLKRLTQGTVLMSLTATQLLTRTQAM
                     MQQVDIRWRSQLAILLENIEALKADNSAWEETPAGKAIAYQLRDYRGAYLFSKLISEA
                     FLPGHGFPVGVVNFNYLTADELEKRRAIKATQADPNEGGESFSRRIEKLPSRDLPTAL
                     REYAPGADVVLGGKVYRSSGIMLGKVLASGQELSGDHHIPWFWHCRKCGAGATSTTHP
                     VECSHCKADIQQLDVKRYLQPVGFATDIRYQAHNDVSMPAQLPWKDPRVLVPSSVWVS
                     LPDAGLGRYRFSHSGELFHFSEGEFGHGYAICLSCGRAESQTQPQRTPENLKNPEREN
                     THYLLRGGSNDRQGSNKLCHGHVHKDLWLGYSSRTDMVELQLNDDNGLLIRDEVAARS
                     LAVALREGLAHKLGIENTELGVTTQQARDINGYTGYSIFIYDNNAGGAGYAVQLIDHW
                     ADVFNYARKLLDCSCDKFCHHCLLSYDSQHYVNRLDRHHALTLLTNVRLQRLNLAPEY
                     QYFGDGSRVETNPLSLRIAQCLNSEIYDSCSLVLAGPQEQWDFAQWPLFKELLQFASS
                     GGNVELLVATPLANLTDSSRHQLSALAAMPGGRLQVKSIATAQLMQGKGRWLAQVTRE
                     GQSQQWAADDSATVAPGELWGQSASSPVVTLKGTSGKTFSGQTLSAEDLLPALPTGAV
                     RINLCEQLDGPLEGFGSRFWSLVTQQHAGWKQAFTRHKEITHVEYSDRYLNSPFTARL
                     LGEILTELVEQGMAERASLTVCVKKLDYNSRQHDALYNAWLNEEDRQQVVTTLLEEGY
                     LGPAWPGAISWLTGDNQSTEHGRELTVTFSDGSQHYVLLDMGLSYWRCIEDTFFDFAL
                     RVPQQVERLANTRARAVAPGNDLRSYIIAG"
     gene            23200..26058
                     /locus_tag="ACHJWA_002012"
     CDS             23200..26058
                     /locus_tag="ACHJWA_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO4308610.1"
                     /translation="MNKQNYAPGMRVVIRDAEWRIRRADDSGDGGYLLTCDGISELVR
                     GKEGLFLTKLEQKVEILDPAKTHLVEDESANYQAAQLYIESQLRQRVPTDSKVHFGHL
                     AAMDSMPFQLDPTRMALAQPRQRILIADAVGLGKTLEAGILVSELIRRGRGKRILVLA
                     VKSMLTQFQKEFWSRFAIPLTRLDSAGLQKVRNRIPTNHNPFHYFDKTIISIDTLKQD
                     IEYRHHLENAWWDIIVIDEAHNVAERGTSSLRSKLAKLLAGRSDTLIMLSATPHDGKA
                     ESFASLMNMLDPTAIANPKEYEYADFADKNLVVRRFKKDVKDQMSGEFPERNIVKLTR
                     LASGAEEEAYRRLVESQFRDDDDEQAQSNKGRLFKITLEKALFSSPMACASVVANRLK
                     RLESRKDHNSQSQINELESLLLALNNIDASQFSKYQLLLDTIRKDLAWKANNTEDRLV
                     IFTESIKTLEFLEQQLRADLKLKDDQIATLRGDQGDTVLMETVEAFGKTQSPLRLLVC
                     SDVASEGINLHHLSHKMIHFDIPWSLMVFQQRNGRIDRYGQKHQPQIRYLLTEASEPQ
                     INGDMRVLEVLINKDEQAQKNIGDSSEFTGKFTQEEEEEQVAEFMMQDDGASLFDQLL
                     NSNVSESAEHDLFGEICSAVSSDASMVTETDTSLFASEQAYCERALGYLKASGQTIQY
                     ETLPDNTLSLVAPEELRRRFNQLPPEIAPENWQLYLSQDKTVITDAIARARGEQHAWP
                     DVQYLWQINPVVQWLDDKISSAFGRHQAPVIRLPYLLEPDEDHFILSGLFPNRKSHPM
                     VNPWIVVSFNRESLIGSQPFAEFLQRHPQLSNKLTNSGGKDRNHQRQQDLLEAAIAHA
                     REVFIHDRNAFETHINQQLNEHLQKLDVLRGRQLSQLELDFADNKQQLSVKQSRKEQR
                     QREIEHNFDSYIEWIEDTMTTEKEPYIQVIAVITGAEG"
     gene            26061..30995
                     /locus_tag="ACHJWA_002013"
     CDS             26061..30995
                     /locus_tag="ACHJWA_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4308611.1"
                     /translation="MALVGINNENEFYSNHYLGEVFTSDIRDVLEPWIAQENAAREAE
                     RAAREQGKDVEPGYRAPWNQFNSLATEFFRKLAEHEKQRQIPQRLADQRNRWQPLLKA
                     LGYEITPQIQMLDDDTPLPVLARYNSTDGSPWLWIVEAHDQEEGTLDPLALSLLTAQF
                     PADTDKHKHDSLRKKANGEYRSWQDLLSTAVFTQNEPPRFVLLLGNRQLLLLDRTKWA
                     QNRLLRFDFEEILSRRETDTLKATAVLLHKDSLLPGSGAPYLDSLDDNSHKHAFGVSE
                     DLKYALRESIELLGNEAMHYLIDRGLANYTGNRAVDPDELSRECLRYMYRLLFLFYIE
                     ARPELGYAPMTAKTYLQGYSLETLRDLEMIPLTSEEDRNGRYFHDSLNMLFKLVREGY
                     NGGVKMQSDLESGDRITIHSHQFSVPRLESHLFDANNTRILNRVVFRNETLQQIIQAM
                     SLSRPAKGRFNRRGRISYRQLGINQLGAVYEALLSYRGFFASEDLYEVKKAGEEFNEL
                     ETGYFVSKDEISKYHEDEKVYEKDGSLRIHRKGSFIYRMAGRDREKSASYYTPEVLTR
                     SLVKYALKELFKEQIDPISDPHAKADAILNLTVCEPAMGSAAFLNEAINQLAEAYLFH
                     KQQAEGRRIPQDRYTQELQRVKMYIADNNVFGVDLNPVAVELAEVSLWLNAISGDAFV
                     PWFGYQLHCGNSLVGARRQVFNKSELTYKKAKDPSWLNSEPVELAMNTPREETQIFHF
                     LLPDGGMANYSDKTVKQRYPDDFKALDSWRKEFIKSFAGHEIADVQRISEKVEALWNT
                     YRQQLKAERLKTADSYPVWPAENSEQTRSSLSSKDETFSGRLEDNSAYQKLRWVMDYW
                     CALWFWPIDKADELPDRGTWLFEIETLLDGIVITEKVTEVAEHTTGDLFAEEGLLREE
                     SSLFSVAGRLKTEVLFRHLPRLAIVDALRKQHRFFHWDLEFCDLFAERGGFDLMLGNP
                     PWLKVEWQEAGVLGDYEPEFVLRKLSASKLATLRIDTFNQIPALEAAWRSEYEGCEGM
                     QNFLNAQQNYPVLRGVQTNLYKCFLPQAWRLGAQKGVAGFLHPEGIYDDPKGGQLRAA
                     VYPRLRAHFQFQNELNLFVEVDHHAKFSSNIYSASPSTVGFEHISNLYAPQTIDACFE
                     HSGSGDIPGLKDEIESEGKLKVVWNTSGHRSRLISIATHELELFARLYDSEGTPAWQA
                     RLPALHAKQLVAVLEKFANQPNRLGDLQGQYFSTVMFDETYAQRDGTILRQTQFPQDS
                     SQWVLSGPHFFVGTPFYKTPRENCTLNSDYDCLDLLTLPDDYLPRTNYIPACDAQEYA
                     KRTPCVTWTELAEDEPKKVTDYYRLAIRAMLAQSGERTLISAIYPPEISHMNAVRSYC
                     YSSQNLLLEHSGMCFSLPFDFICKSTGKANLHQMLDGFSYVLFNPRQKALLYCLVLSL
                     NSVNDVYAGLWQSCYTPDFNTQRWSRDLPQLPQDFFAKLTPEWQRNCALRSDYSRRQA
                     LVEIDVLVAQALGLTLEELLTIYRVQFPVMRQYEADTWYDQNGRIIFTPSKGLVGVGL
                     PRTARKADLKNGFVFNVDSPEWTGGDCTDQAIGWDDVKHLKTGTVSVTFDDYTRSDEG
                     ERRTVTWQAPFIKPDREDDYKVAWAFFAQDKESA"
     gene            30995..37336
                     /locus_tag="ACHJWA_002014"
     CDS             30995..37336
                     /locus_tag="ACHJWA_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO4308612.1"
                     /translation="MLPSVVSRQVADSVAAFLRAAFPLNSPLFNGENNDNVSMLEQFL
                     AQPETLLKGPYLSAQLPFRKSDLPLNFFPNLTLPFPPHAHQARAFQRLGSDAPQPTLV
                     ATGTGSGKTECFMFPLLNHCAGASQAGVKAIIIYPMNALATDQASRFAKTIASDPQLH
                     GKVTVGLFVGDSEIEPSKKMSAEKVITCKHTLRESPPDILLTNYKMLDYLLMRPGDQP
                     LWRYNQPGSLRYLVVDELHTFDGAQGSDLACLVRRLKHHIGVDNQRFACVGTSATVGD
                     ELGQLLDYAKTIFDQPFTDDAVIREDRYTAAEYLQNYAIAYSQYPGPEVASALDPQSY
                     ATPVAYLNGQIPLWFPDSDLQLPDDLESDDGREKRIALGSLLRRHSVMHVLLHDLQGG
                     ILSERECLENLQVMLAESADHARRVLQSILALIAQARLEVPETEHDRQKRLQAKKARP
                     VLPFVQLRSQLWLREMRRLVASVAKTPRLVFADDVAVEDREHYLPVIHCRDCHATGWA
                     SLRHGQSVQLETDLDGIYRQFFEKGRSIVLAFPDNNEKAVSGAHQKLCPTCLKLNRQS
                     SVQCGHCGHTELLQVLIPDMLKERKDELEFANECPYCNSKQGISILGSRAASLSAVMI
                     HQLYGSQFNEHKKLITFSDSVQDAAHRASFFTARTWPLMIRGQIASVLSEKETLPLDA
                     FAARVCQQTRERSPDDAHFAANVIAPNMQWLNDYQTLKNEGKLAENSDLPELVSLRLD
                     WEVYSEFTLRARIGRTLEATGYAVAGVDKDRFNPAITALHQQLCEELGDEMSGVSVEQ
                     VAHLALGVIWYQRQAGAVMHPAFESYRRDGTTFMMTQKERTSRYMPSIGPKTRKPAYA
                     SFEKINGFHRLIGAKSNPSWYQHWINRTLSNGNNLFISSVAETVLRRLFTALKIAGVV
                     KDFDTKGREAWGLVPSALVVSRDVVQLNCSCCREVVRAPADQGWQWRNAPCLSLRCEG
                     RYQEVDNTATWRWENMDIARVQGAEHTGLLSREDREVTEKSFYRGNQPWNINLLSATP
                     TLEMGIDVGDLSTVLLCSVPPAQANYLQRIGRAGRKDGNALNITVAEGNPHDQFFFEE
                     PLEMMQGQVQAPGVFLNATAILERQLAAFCMDNWVKTGVPESAICKNVKQMLDELEFG
                     RKSGFPYNLLRYIEQHHAEIAAQFTAIFPDLAAETRQQLLSYLQGAPGQRSLVQRIEE
                     ALKLLVEDRKSLRSRIDKLKRSIDKLENAPRDQNFDSDMRELTSERQALMALVNQINN
                     KQTLNFLTDEGLLPNYAFPEAGITLRSVLWRRKEGGEAREYQNTTFEYERPASTALAE
                     LAPLNNFYAGGHKVEIEQIDLKVSEPENWRICSHCNYSENIDQTGDQHKYCPKCGTPG
                     WADAGQKTTLLKLRQVYARASARDSQISDESDSREPAFFQRQLLVSFEKEDVSAAYAI
                     EEGEVPFGFEFLSKVTLRDINFGKMADDANELMIAGEAKKRTGFKVCLGCGMVQRPRD
                     HEPRHDLSCKYRTEPEKAKFEDYLYLYRQLESEALRILLPVSSYSNDRVVEASLGAAI
                     QLGLKHYFKGNVDHLKGVVYREPENEGESWRQYLVIYDTVPGGTGSLKELMRTPDNLL
                     KLLELAYQAIVECSCNNDTHKDGCYRCVYAYRDRGRMKYVSRDQARLLLAKILQARDS
                     IRVIDSIKNISLEAMMGSELEKRFISCLQENKNLIVSRSYAHQGAGWIINTRSEPMMS
                     WHLKAQVDLGAKEAVGIPCRPDYVLYPLMQSDAIKPVAIFLDGFAFHKNSVAEDVQKR
                     QAIRDSGNFWVWTVTWADLKEPGLKHVQDVLGLGHNPDMKQPKFYNLFHDTNFAALEA
                     SFRERNSFALLLDYLADPGGRTQLWQRMAAAHAWVWLDVKKSQDAGTKQKYAYEMQEN
                     APAWRLAELLPDEPFVFGGLLDSCNSSQQFIELASVLPQQAIKPTTSVAQMRSWLRLH
                     ICFDDRYTQDDGYEAGLNGFWRLVNLLQFLPDMTFTSRKAVHLPQESVDTKVSATPEA
                     VPETDESWAEIIEFGLLSAEEIALLQSHSLSAPTLGYELQNDEGEIIAEADLAWPSQK
                     WALIIDNQEFIPLFTTRGWHVAFGPIDENTLQHLSGGDK"
     gene            37333..39447
                     /locus_tag="ACHJWA_002015"
     CDS             37333..39447
                     /locus_tag="ACHJWA_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFO4308613.1"
                     /translation="MTVQTPKVALSDGFLGAFARIPKAQQKKVQEFISKFRQDPTSNG
                     LNYEKIHDARSKNVHSVRIDQTYRGIVLKPEQGALYMLMWVDKHDEAYDWARRHDCSI
                     HPVTGAIQVIDISYIKPAAETVVDKPKLFAAYSAEQILALGVPPVFIDQVMALTDEAG
                     LNQLESIMPAEAWEPLHWLAEGLDYQEVLEEFNDHRDEPVDTNDFMEAIERSKRRFHV
                     VENEQELLQMLNAPLEKWRVFLHPSQRKLVESPANGPVRVLGGAGTGKTVVAMHRARW
                     LSQRLADKPGKKVLFTTFTRNLAADIRANLQRLCTREEMARIDVVNIDAWMSDQLKRH
                     GYDFRVVYDSDEGRRKCWNYALQQAPAELGLPDNFYAEEWQRVVQPNAVYTREEYLKV
                     SRVGRGTALSRIQRAKIWPVFEEFRAQMARAKLREMGDAMHEAIVLFKEKQVQLPYSS
                     IVVDEAQDIGAPAFTLIRSLVPESPNDLFIVGDGHQRIYRNKVVLGQCGINVRGRRSK
                     KLKINYRTTEETRQFAVGLLTGVKVDNLDGEADTSNDYLSLLHGEKPMITHAADFKEE
                     AATLVKQIQALLANQVRSQDICITARTKHACDRYASALNDAGIETFNLGNDSGDSDAR
                     PGVRVATMHRIKGLEFQYVFLVGINEGVVPEIKALASDDPVEQRDALFNERALLHVAA
                     TRAVKGLFVSSSGVPSPLLVAD"
BASE COUNT        10361 a         8995 c         9773 g        10604 t
ORIGIN      
        1 aattgatctg acggttttct ctgacagcat acaatcaagt ctaacagtcc gccttgagcg
       61 aggagcggac actcatgatt cttacttata tatattttcc tacatcgaat ttttttaggg
      121 gaaaattatt tagttagtca cactccgcca gtcaattctg cgattagtgg ttcataaggt
      181 gcgccagctt gtagtgcatt ccagatttct aaaattttat cacgatttct ccccaaccaa
      241 ctcgaaacat cactatcata tttcttaggt aaatctccct ctatgcggtc tcctgattgt
      301 atggcataac tggcagcatg cgcttgtttt ccatagtcaa tatgaatatg gggaagttga
      361 tgtccttttt cttgatatat ttttatcttc atgttctgtc tcttcaccaa gagaatttcc
      421 atgaatccgc cgccactaga gcgtcttttc tcagtcagta gatccttctg agcaaagagt
      481 ttttgtaatt cgtaaaattc ttcatctaaa ttcatatatc acccattaaa tttcagttag
      541 tagttattaa actcaactct ttatcgctag gcttttccta gtccactttt ggcacaaagc
      601 tgtttaggtc aaatctaagc taatacactt gattacttac gattgcatcc gcagcccttt
      661 cacgctttct gatggtaggc taatcactgt tttaatgcag ttctctatgc tgccaatgat
      721 gtcttcgcgt gggccgctgc gccagtctgc gcttgcgtaa aaagcatcga gaaccgcagt
      781 catgctttta gcgctgtcaa aggcgcgaat caagtagtag caatccaggt catcaagcga
      841 gttgccaaac gaaacaacat cgatcccgtg gctttggtgt aggggaacac tgatttcctg
      901 cattatggcg tgaaaagcgg caccgctgcc tttgcgtaag gtgtattgca gtatttcgac
      961 ggttctcaag cgttatctcc gtgttgccct tgggcggctc ctagttgata gcaataaagt
     1021 aaaccggact gggccatgaa gctactgcta atcagacagt catccgtaag cagaagctgc
     1081 gatatgcggt tcaaacgcgc tgtaaggctc tctggcgagt gttttaaaga aggtagtcga
     1141 aatcatggtg cagaattcgc ggcgctctca tagagtgact ggtgaagcca gttgaaacaa
     1201 gcgtagcgga gttcgagcaa tcaatgagaa atctgggcga gctttagttt taccgcgtcg
     1261 cttaactttg gaggctgatt gcctctataa ggactgtctc accgccacgg aaattatcgg
     1321 atgcaatgct taagaggggc gcttccaaac ctaggacatc cactttttcg ctcccaactt
     1381 atccggctgc atttacaccg cgaacacgtt gaacgttagc agcttctggt tcgtgtttgg
     1441 ttgttacgca tgtgaggtaa aaacggcagc tttacttttg acgggaacgc cataagacac
     1501 caaacagcag agaagtgtaa ttgttaaaaa tgttaaaaca taacttgttc tttaagatgt
     1561 aaacccttcc tcggggggga agccggacat atcaacccta aggccatctg agaagatagc
     1621 ctgtaggttt gctcggttcc ccttgccaga tgaagatcaa gtcttcctcg tggtgtgacg
     1681 acctttcttg aaagatgcat tatgcgcaaa agaagctact taagaatccg ccgatagcta
     1741 ctttcaccct ctgcttggct gcatcaaacg cttgcgcgcc ctacctacca caatcaacgt
     1801 tacgcagagt tcccatcctg acgtcttccg ctaaacagcg gtgatctcca cctacccgga
     1861 ccggtttacc gcatgaggta ggtgtcagga gagaagctag cacaccatca ttttaaagaa
     1921 aagtcatata tatcatatgg ctgcgttcat attctgaacg cagttatcga tgttaaccta
     1981 aaggtatgag aacactgtcg tttttcatat acaaagggtg cttcggctca cctgtagcgt
     2041 ttaagacgaa gcacattgtg ctctctggca atagctgtcg gacggtcgtt gagcgtttga
     2101 ggtgtcttcc ttcatttccc catgcggcga caaccagtcc ggcaccgcta acaagacgta
     2161 tcaagtggct atcgttatct ttgccaatgg gatcagacgc tttcatcatt tcatgtcgct
     2221 gggtgtgaac gaatgcgaac aggttggcca tgtatacgcc accgtagccc cagcttttgg
     2281 caaacgatat gcagcggctc aaggtgcgat catccttctc ggcatcggcg tatgaagggt
     2341 ttagccctat gaaaagggta tacggttttg attcgtccca gactcgtgat aacgagtagc
     2401 gatacttacc gcacggtgaa aaaatcgcgt ctttaatcat ttcggttgtc cttatgaagt
     2461 gatggtttca gcctaacatg tcttcacccg ttttaagcgc caacaaacga ccgttttccg
     2521 gcatcctcca accgcacgct ttcacactgt tggaaaatta gccttggctt ttccggcagg
     2581 ccatcctatt ttcatcaccg ttttctggca ggggccggat gaggagatga ctatggctat
     2641 ttttggctac gggcgtgttt cgacatccca gcaggatact gagaaccagc gcatggaact
     2701 tgaacaagct gggtggacgt tcgacttttg gttttctgat gtagtcagcg gaaaggttcc
     2761 tgcggtgcaa cgcaaagcgt tttttgaaat gctcagcaaa atccgcgatg gggaaacgtt
     2821 ggtagttgca aagctggacc gcctcgggcg tgatgcaatt gacgttctgc aaactgtcag
     2881 gacgttggct gaccgaaaca tcaaggttat tgtccatcag cttggcacca ctgatctcac
     2941 ctctgctgcc ggtaagctcc tgttgtcgat gctagcagcg gttgcggaga tggagcgtga
     3001 cctgctgatt gaacggacta atgcggggct gttacgggca aaagctgaag gcaggaaatt
     3061 gggcaggcct gctaaaatcg caccggaagc tcgtggagcg attttagaca aaagggccgc
     3121 tggggttagt gttagcgcat tggcgcgtga atacggtgtt tcaagagcga cgcttgctgc
     3181 attactgaat aagggttact gatatactta aaacatgctc taacactttg cagatacgag
     3241 caatacttgg taaaacagca cactttccgc cagaatcgga tttttgaggt cctggcaaca
     3301 gctaattgct gagaacattt cagtaaactg tttaactcgc tatcagccga acactgattt
     3361 gtatagaaat gctaccctcg ttcatactat caaaaataaa ttaattttga tgccagccag
     3421 gttggtcatt ctcaaatacc tcagcctcgg ggaatatttt agtcaatggg ataacatatt
     3481 tgaccagatc atgggtaaaa aagtcatccg cagtttcatc taccatccac gcagagttct
     3541 catctatgcg tggcacgaga taatgaacat tcaggcggac tattttagat ttttgctcaa
     3601 aatttactat ggcagcatag tttttttccc ttcgtttatc ggcaacatcc ttttgaatat
     3661 ctacatccaa agctatatgc cctacgccta gaccataatc caaaagctgg ttatctatct
     3721 ctgctaatgt ttttgttaca tgtcgcgaac gtgcatcgaa agatttttca ttgatacaac
     3781 gccatgttat caaagaagca agtttaactt tggttatgaa acgtgagtcg cgctcatctg
     3841 gtcgtcccat agcaacaact tggtaatttt catcttccag aggggagcct ttgataagac
     3901 gagcaagttt agtgttgact agtaatgagc cgttttcagt gatatcttcc ctgataagat
     3961 ttaagttggc cggtctaatc gttccttttc cataatcatc attccattca taaaatacac
     4021 ctttaaagtt ttttaagtgg ctaatgatat agttttcagg cgcgtcttta acttcacata
     4081 aatatgtgac atcagtccat acgttcatct ttttggattt gatatgaagt tcggcaagat
     4141 gtgagcgtat atgatgctgt aatatttcct gctttacgta aggacctttc tgaagccttt
     4201 tgcattcgac aaagaaataa tcgccatctc caaggcggca acgaaactcc ggggtttttg
     4261 ctatcccttt ttgttctgga atgaactcaa cttcataacc ttctgatgca tagtttccag
     4321 caagaaccaa ctctaataag gctgtgtctg gtaatactgt tgtattctta agcattctga
     4381 ttgcgcgatc tctagcgcca gtaattctat cgagcgattt agcacacact cctaattgtt
     4441 taacccatgg tattatattt gaagcattag taacttcgta agaccttcta ttatcgataa
     4501 gcgattttgc ttgtgcgaaa taaccagcaa ccacgtcgga accataccat tctgggtcaa
     4561 actgtttccc gaaattggca gctttttggg ttgtagctat gtaaaattgc tgtgcttttg
     4621 ctaagcgctg gtaaaagtgt tgtttgttct cttgagttga tgctaaccac tcaagaccag
     4681 caataacgtc agaatctagc ataacaccgt cattcatctt tattccttag ttcagccctg
     4741 aaaggcacta gatcaactga gacagattat agttacggaa agataacgcg atgaatatac
     4801 gatggtaagc ctccgctttc cactctatgc tggcagctga gttctatcat gttcaggcac
     4861 ctacgtttct agaagttgtt catacagtta gtataaatat acgtccctga aatctttgcg
     4921 tcttgctttg gatgttcata tacgtagtca acaaaagtct tatagagcgt tatcatctgc
     4981 tgggtgacgt cggccttacc gttcatgtta tcaagtcgct gataatattc tcttttcgtc
     5041 atcccactgt tacggccttc ggttacgttt gcggtaaatt gagccatacc catgcacatt
     5101 tgatctttat tgttattcat ttgatcttta ttgttattcg ttttatccgg gtggagagcg
     5161 ccttccaaca ctttatcacc gctgatggca tgttgcgccc acgctgaatt gttagcgata
     5221 aactttttgg agccgatggc aataccgtat tcaacgcgaa ttccattgtc gtgataactg
     5281 ccatctgcgt cttttgtcgc tagctttaaa gatatacggc aaccgggaag cgttttgtgt
     5341 tcttgcaccg agataataac ggtgtccgta tcgcaatcag caaagctgta ttcggggtta
     5401 acgtaagtgt tagttttgat taacagcccg gtaagaccgt ttttatagcc ttttgccggc
     5461 agcgttttga aggcgctgcg ttcacccttt ttgatcgctt cccggaagtg atccataccg
     5521 atgcgcgtcg ccagttgatc gatgctttct gctgcataag atgcagatga cgcagcgatt
     5581 aacataatgg tcagcacaga cttggctgaa aaaccgggct tactaagccg tgtttttgac
     5641 atgttgttat ccctcagaat ccaccccagc ggtgtcagtt atcgcatcat agaccattga
     5701 ttcaaaatgt cactattgga gcgtggctct ttagtggaca tctgcgcaaa ttcaggtcat
     5761 gaaaatgacc ctgagaaaac tgtttggaca acgagtaaaa gagttgcgca ttgccaccgg
     5821 aatgagccaa gaagcctttg ccgatcggtg tggttttgct cgtagctata tgagccgcat
     5881 tgaacgcggt ggctctaatg cgtctttgga tgcgattgag gtgctggcta atgctctgag
     5941 tgttgagccg tggcgactat tggtgtctga ttcatctgaa gatagtgacc cggaattact
     6001 ggttccttat gcagctgatg gctcgtgttt tcatcccggg ctggcaagca ctcgcgatgg
     6061 ctcgtttggc gtaggtgata aaacggctca gaagcggttt ggcacgtttt ctgaagctct
     6121 tgagtatctt cgtagcatgg agacagcaaa atggcgtagg cctaatccta gcggcaactg
     6181 gggtattgta tctgcggttc ggtgggacaa gctgagaaag tagctcaagt gtaagtttat
     6241 gaattttcat gctgtatgga tgaacagtgt tcattagatg ctgatagcct cacgtttcat
     6301 cagctctttg tttctataag agtgaataaa acagcaaaat gcgggtgcgt tacgtaggcg
     6361 ttacggtggc gttttacttt gacgctgaat ggcttaattc atataaataa cattgagtta
     6421 aatagttatt ctgaacaagg tctgacgggg tttaactggt ttcaaaatcc gaacgcagtg
     6481 gcgtttactt aacatccaca tagctgacaa atgttctttc ctcccgaccc actagacctc
     6541 cataaacatc aaaaggcagg tatggcacgt taatgatttg tgccgccacg aacactcggt
     6601 ttgattccgt ccagcggatc aaacttaaga tgtagcccct agaacaaaaa ggtcctacga
     6661 ttgttctttt aaaggctaat aggaacgtga ttacagcagg ttatggagac ttaggttcga
     6721 gtccggcctt cggcaccaaa agtatgtaaa tagacctcaa ctgaggtctt tttttatgcc
     6781 tgaaatccgc gccacgcaag gcttttccag cttttctcct caactgaagt caacctgaac
     6841 caacccacat caacaggctt gtgagtatac taatgagtat atttgccgat tcgatattgc
     6901 ttgtatactc acgagatacc aatggaagga agaccattat ggccctaacg gatactaaag
     6961 ttcgctctgc gaaacctgag gagaaagagt attcacttgt tgacggcgac ggcatgtcct
     7021 tacttgtaaa acctggcggt tcaaagtatt ggcgatttcg tttccgtttt ggcggtaaac
     7081 aacatttgat ggcgttcggc gtttacccgg atgtttcact ggcggatgcg cggaagaaaa
     7141 gggaagaggc cagaaagctg gtagctgctg gtatcgatcc tcgtgagcat aagcgtgctg
     7201 tgaaagaaga gcaggcgaaa gagattatta ctttcgagaa ggttgccaga gagtggctcg
     7261 taaccaacca aaaatggtcg gaagatcatg ctaatcgtgt aaaaaagagc ctggaggaca
     7321 atatcttccc gacaattggt actcgcaaca ttgctgaact aggcacccgt gatctgttaa
     7381 ttcctattaa agccgtggag aaatccgggc gtcttgaagt ggcttcccgc cttcaacagc
     7441 gcaccacggc catcatgcgt tatgcagtgc aaagcgggtt aattgattac gatccggcgc
     7501 aagagatgtc gggggcggtt gcttccagta atcgacagca tcgtccggcg ctggagttaa
     7561 agcgtatccc tgagttgctt gacaaaatag acagctacac cggcagaccg ctaacccact
     7621 gtacgacaga actcactctg cttatcttta ttcggtccag taagctgcat tttgctcgct
     7681 ggtcagaaat cgattttgaa acgtcaatgt ggaccatccc tcttgattgg tattgcagta
     7741 agcagagggt tggactggta cagtaaatat cccgcataat cgtgccattc acatttagag
     7801 atcctccggc ataatcaatc tgccaatgaa ggagatcgct attcgtaaag ccccttttac
     7861 tgagcatcag attatcgccg tgattaaatc gcttgaatac gggcgaactg ttaaagatgt
     7921 ggcttatctg aagccactta ctacaactgc aagtccagat acggcggtat ggaattttct
     7981 gatattaaaa taatcaagga tcttgaggat gagaaccaac gtttcaaata gatgtttgct
     8041 gatctcatta tttggtcggg atttttaaag atgtatggaa ctaaaacggg tctattttca
     8101 gagggattta actgaagcag aaattaataa atcataattt ataggtggct ggtatttaat
     8161 attcctgcat catatctctg atgcaggagc agagaatggc tataaattat tagtaaacag
     8221 aatgtctatc tctcacccac aaacccctca taagggtaat ttttacgcac ctgcggcaac
     8281 gcaatagttt tcccgtcaaa atcccagaat aatctctcaa cgatccctcc gccgtttacc
     8341 gcatccgaga ccagtacccg catattttca tccaggccaa tcgatccttt atcaaaggct
     8401 ttatggtgta ttgcgcaaag cgccagacca ttggggattt cacaagggcc gccgtgctgt
     8461 ttccatttta tatgcgccgc ttccagcgcg acggtggtgt catcgtggcg catattaaag
     8521 ccacatattg cgcactggta gttataagcg cgtaatacat ttttgcgaaa taacgggtcg
     8581 cgctgtttac gggtttgctg gagatcaaaa cccagctcgt cagcgatttc ttcctgaata
     8641 ctctcggtga agtgcgcttc gagtatctgt tgcgccagag tattgatgag ttttttattg
     8701 ccggttacca gggcgtagtg ctgttcatcg aaaccgcctg caacgtggta ttcgttcagt
     8761 tccttcactg gcggttgtcg gctgctccct gctgttgagc aacgctcggc attgtgtaat
     8821 tgccagattc cgtcgccttg taatcgccag aacggcatat caggtcggta ctgtgaacgc
     8881 tgtggcccaa aacgttccag taaactgtgc agaggttcgt agatttccgt gccataatcg
     8941 aaaaggcgcg gatgtccatt caggtatccc gctaatacgt atagcagtaa caatggttta
     9001 tgcggcgcgc gctgttcacc cttgtgccag atttttatgt tggcaattgc ctgctgtagc
     9061 gatttactgg aagccattga tatcccaggc agtgaaataa ttgcaatcat gctcgcaaat
     9121 tttctgctgt tcaagcctgc aagtgaagat ccattgctgt gttgagggat caaaagacaa
     9181 caaaaactta ttgaagaaat aatcttctgc ttttttgtcg aaataatggg cattgatctt
     9241 gtctatagct gctgaatctt ttccagcctc gttgagatct tcaaaatagc cattgaagat
     9301 gttctggtta acaccgccag taacataagc cttgccgttg tgtacaaaag tcacattacc
     9361 cgccatgccc atcggcgcgt gcgacatcaa tttattgctt tattcattgt gactgtcttc
     9421 tgtctaccaa gctttaacct tcacccatat gagggaagtt ttaagtatga ggcgaaccat
     9481 agcatatgct tttatacctg ttacaccata aaaatcagac aagttatgcc ataaactact
     9541 tttgtaattt gagccacaaa tcagggaaat tttatttatc ttccgtgaaa gtcttaactt
     9601 tcacggagtt ttagaattta caattttgct ggcgtaaagg ctcctgtttg tatttcttta
     9661 accagctcac ccacggtata tattctccat ccttcgactt cggcgggagt aaggttaact
     9721 gcaattttaa ggcctggcca tgcagcttct acggttgcaa taatttcatg ttctgagttt
     9781 ggaagatcta acccaggaac aggaggttga aagcctttat gttgcatcat attaatatca
     9841 ccatgcactg atggatcagt caggtcatat atttcattcc aggattctgc ttttaataac
     9901 ccttgttggg aacgtaaacg ttgaatgata gccatctctg cttgggtaac gaatatacct
     9961 tcgtggaggg gctgttttcg atggcaatcc ttgcacagaa caagaaggtt ttcatggtga
    10021 ttatcatatt tgatgccatt tttatgatgg acatggcaca agtttttggc ggttgataaa
    10081 ttcacgccac aatcattaca aacataattt gccttttctc gtatttcttt agatatttcc
    10141 ttccagtttt cgacataccc actatttgtt ttgtcataga tacccttagg catatgacga
    10201 aaacaagtac tataatctga aaaaaactca tttaatgaga atgatttaaa aatctctctt
    10261 tttcgttgtt tatcaatact tcctttataa ttaagtcgtt cgaggcaatt catacatacc
    10321 cgtaaatcaa catcctggct ccgacctgaa ccatcatcaa tttcgaataa accatctatg
    10381 cggttagttg catgatacct ttcaaatcga tttttatggc gcatttcatc aagagttctg
    10441 caccaggcaa tatgaaaacg ttttcctttt tcgccatcta cgatagccgc atcataacgc
    10501 cctgaatggt cccgaatata taaaagaacc tggcggccat gataagagat aagcccgcta
    10561 ccagggtcaa gatcttcgat actaacttct ttacccgtgg ataactcaat atctatagga
    10621 tcaaagtcaa taggggattc atccagaaaa aaatcgactc cttctggtaa cattttcttc
    10681 cctaattgaa taagttctga aatgtcgata gataatttca tctcttactc ctgaataatc
    10741 tttctaatct gcaactcggc atttgttaca actttgaagg tgacacgacg agagcgatta
    10801 ggatcctcct tgccggtttt atcaagaatg ggatgtgcag atgaataacc tactgcggca
    10861 aatttacttt taacccattg ttggtgtgtc gcgatatttt ttatgtcata aacgtattgt
    10921 aatactgcac gtgtacgacc ttgcgatagt gccatattat taaaataagc aatatcagga
    10981 ttcgttgttc ctgtccagtc agtacttgtg tgaccttcaa tgcggacttc agtaatatgt
    11041 tcctgataat tatctagaac ttttaggtag cgaggaaaga agtcgtcaag aatgagttta
    11101 aactttggtt ttagctctgt gcttcctaag ccaaataaaa catccggtga tttaaatcga
    11161 acctccagag tctgtttatc gatctctgca tcccagtctt gtaaatcctt tgcaaactca
    11221 atatccagag cattataaag ctgtaactga gcattctcgt aggctacggc aacttcttta
    11281 attttttctt tttcaatacg tacgtagtgc atataagcaa tagatatgaa cataaaaacc
    11341 atcatcagcc cagccattag gtccgacatg gatacccaat gttcattatc ttgatctttt
    11401 ttaggtaatt gtttccctat aatcttatcc atgatcgatt acccgcgatg tttattagca
    11461 gaaattaatg cttgtaactg ccccataact atttggtaat ctttaataag tttttcatag
    11521 ttaccgacaa aacctttgct gattgaaagc agagcattac ctaatgattg catttcacgg
    11581 ttcatttcac gttcagttgc ttgctcaaaa gcatgtaact gctcgttgat ggatagagca
    11641 aatttttcaa ttgattctct gacttgagaa gtcagccctg cacttgcctt atcaaatgat
    11701 gtctggattg aatcaaacat attatcggca ttactggata gttgatggtt aatcttttca
    11761 ccagtcttac tgaacatatc aatcacattc gtcgtatttt gttccatatt tctatgcaat
    11821 tctttcgagt gcgactctag tgatttcagg aattcttcac cactttgttt catttcagta
    11881 attgtttcac ctaacgtgct ggttagggtt tcggaggaat tgctgacatc atgtgccatc
    11941 gaggcaaatg cttgttgtgt ttgggtaacc gattgtctga atccttcggt accttcatcc
    12001 agggcaacgc gcattgaatc tgcattaaga agtatttgct ggctggtttg ctcaagagat
    12061 gcactaacat ttgcagctcc ggatttcagc agttcaccca cttcagccat tttgttctgt
    12121 atttcaggta atacggttgt agctttatcg cggatggcga caaaggtttc taaatggcgg
    12181 gagagttcgc tgatttgatg ttggttcacc tgaagcactt cacgcagttc agacatagcc
    12241 agaggaattt ctttacattc ttcccaaatc cctgcaaccg cagtttttgt ttcaaccagg
    12301 gactcgacac tttgttgata ttgttctgac atctgctcaa tttgcgtttt ataatttccc
    12361 tgccactcaa caagtttttt tacagaggca tcaagagctt taaagttttc accaaactgt
    12421 tcagttaaat tttgattaaa atcaataatg acttgtcgca aagcatcaat aatttgttct
    12481 gtagcacctt ttgccattag atctgcaaat tgttcaagtt gttcccaaag cttattactg
    12541 aaatgagtgt gattagctaa ttgtgcctgc gaggaatcgc taatctcagt acgtagtaat
    12601 tttatttgag caataagaga accttcacta tcaccgttaa tacccgagac taatttagtc
    12661 agagtctggt tctgctcttt taattcatga tagatatgtt caggtgtaac agattcaggg
    12721 ttattttctg ctaacgcact tcgtttattg gcaaaaaaga aagcatccat tccattaaat
    12781 aaaatggcaa aaaacatacc tacaatgctt gtaatgaatg ctgtttttag gccacctaat
    12841 aatacgggga tgctggtatc aatactttcg gtattaaaat tgagtaaacc aataataata
    12901 ccaatgaaag tacccagaat acctaatgcc cccattaagg ttggagcata ttctctaaac
    12961 ttactatgta tatttttttt gtcacaccac cacgctgata aaaatacgaa tataacagcc
    13021 cagatgaaaa tattggtgat cagtgtagag tctatcgatt gaaacaactg ctcaaaaagc
    13081 tgcgctaaca ttttgatacc tgtgtaaata atggatagtt gtaatcgttt aaaaaataaa
    13141 caaaacacac ataatttata tggttaagtt cggtgtagcg ctcatgattg taacaagttt
    13201 tttcgagata taaatgattt gtctcatttt tagcgagttc atcgggttga gcttttttat
    13261 gatgatagta acctgttacg aattgtatca aaggaaggca ggttgcttgt gaaaactcca
    13321 gttaacccac ttttgcaatg gcttaacatg ttttttagtc gccgttcatt atccggagct
    13381 gatggacggg cgttatatgc ttaccgttgt actgatacag agtacgaaag tttggcagaa
    13441 ctactgcgta catatgcccc ccgtagttat ccaagaacga tattcatttc ctatagcgat
    13501 gttctattta gcatatatgc cgcagagttt atccgccgga ctcatacggt cggacaccct
    13561 aaatgggaca caattttaga ttctattaac tggaaagttc cgtatgttca tcgacagaag
    13621 ctggttaatg atggcattcg ctactggaaa agaaagataa gaaacctggg acaagcttcg
    13681 ggttatctgc atacactggc ttgtgaaggt ggtttgccta tccgcatgat tgaaaatgag
    13741 agcggttatt tgattaccta tttcagaagg atatatcagg cgcttcgtgg gcaatcatct
    13801 caatatcctg ccgctaaaat tgcacaagaa ttaggcgata cgattcctgt cacaatgcaa
    13861 aacgaactgg tctatgaaat agccggtgaa ttctgcgaga ctctctgccg attacttagt
    13921 gagcatcctc ctcatagcag tgatcccgtt tctgcattac gtaagctctc tccagactgg
    13981 catcttcaac ttccactcgt tctccccgaa gcgaatgctg cagagattgt aaggcgatta
    14041 ctttctcaat cttctgaaat acgcagtgca agtagtcttc aggttgaaag aatctgggtc
    14101 gatgttgatg acagttggta ttgcgatgcc cggttccgat tcccggccac aatgcgtaca
    14161 gaacagttaa cctctttgtt tgaatgccat attcagccgg agcagacccg actcatcatt
    14221 tcaggaaaat ggaaaaatgg cggggcaagg ttggcaatgc taagccgtta tgagcagcaa
    14281 gattggcggg tcgagttatt acctattgcg atgcaaaaac tctctggtgc agatgcaatg
    14341 gccgaaatct cattgtcgct tcatgaaggt ccaattctgt taggtcacac aattccaaaa
    14401 ggcggttatg aacttactga agaacttccc tgggtttttg aagcgatgaa tgagagtgaa
    14461 tcgcagctaa aacttgttgg tatggggtct gtgagttcca ggctaaatgc tctgtttatt
    14521 tcgctaccga aaaatagcca tttagatatt agtggagaag gtgagtttga tatcccaagg
    14581 ttgctgaaaa acagtgaacg cagcttaact aaaataagtg gagtatttag cgttgtttta
    14641 catgatggtg ctgtttgtac aattcgcacc cagcaacttt atgattctgc gattgaatat
    14701 tatattaaat cgacagaagt tgaactggtt aaatcggatt atcctgtcca tcgagcctgg
    14761 ccgaagatcg gttggaaaaa ggatctgcaa tatggcattg taccggagaa agaacttttt
    14821 tggcgttcca ttcgttcagg taataatgcc tggtattctg tcgcatcaga aatgccaaaa
    14881 ggacagatag aagtccggcg aatagttaat gatgaggttt tatttagcgg taaagttgta
    14941 gttttaccag cagacttcga tattaatatt atacctgaga gtgctcagca aggcattatt
    15001 atgctttctg gtataacgga tactagaatt gataaatatt caaacaatga aaaagtaaca
    15061 cttaagtccg attattcaca aaacgagtgt gctatttatt ataattcatc gctgatgctg
    15121 gaaaataccg ttgatttacg ggtctcctgg aaagatggtt ctaatcttaa actattatta
    15181 cctaagccgg ttagcggtgg ccgatttgta actaatgatg gttctgttca ttttgatggt
    15241 gtggcatcta tagcacattt gcatggaata gatgctgagt tattaaccat atcatgtgct
    15301 ggaagaggat atcttaatat cgagttattg gatgaaaatc cagtagctga aaaattccgc
    15361 tatttacatg ccgaccttcc tcttttatca ggacgcaatg acaaattaca acaaatttca
    15421 ctttatgaga actataattt gctaaatgct atgctggcat gtgcatggaa cagcaatagt
    15481 acattatgtg ttgattttta ctctgacaga tttggaaagg ataaagcaac actcaatatt
    15541 aaacgctacg atggtagttt tattgaacac gatcaagggt tactggttga tataaaaaat
    15601 tctgttgttt ttcctgcaaa tagaatagac gaactggttg ttgatgctat ttctcttaag
    15661 aatcctggct tacacatatc attgttaaaa aaagatgagt ttgcttatga tctttcagct
    15721 ctgaatgttc aggatagtcc atggttaatt gtgggaaaac ttgatggtac agctcgcatt
    15781 gcaccagtaa ttaaatggat gctacctgta ttgcagacaa atgatttatt actaaatgct
    15841 ctatgcgaag cagacccaga acagcgcaaa aaaaatttta atgagctaat ttttgaaata
    15901 gataacaacc cattgcaaaa ttattgctgt ttattaacag agtatattaa gaaatacaaa
    15961 atgaataatg gcttatcctt gctggatctg gacttgttca gaggtatttc gagtaattac
    16021 cgcgtggttg ttcaattgtt aatatcatca tgtctttctg gtgatagcga tacgatttat
    16081 gatatacagg aagaattacc cttttcatgg ggatggattc ccgtttcaat ctggaaagat
    16141 gttttccaaa aatgttggac ttatctggag aaacagatta acgataaaac attagcatta
    16201 catatattgc aaccctttat tgcttttatg aaccatcgtg cacatatcga tcgtcggctg
    16261 gctccgattg cgaatatgtt acttacatat agtgagagcc taccaaccgg ttgtgatgta
    16321 ttgccaactg ttagtcgtga gcagtttaat gaagctaaac agatgctatt aaggaacccc
    16381 gacagctttg ggcgtatcag tatcttccct aaagaacttt ggtctagtgc tattactcca
    16441 gagttaaaat ctgtttttaa taagctttgg attgaagata aatatcactc acggcttgaa
    16501 aaacgtttta atttgatgtt agtcgcagcg ctgttaaccc aaaaagataa taacctgata
    16561 catcaactgt ctgcgctttt tgaatttcac tatcagcaag ccccacagca attaggggta
    16621 atctatcaat attattttga acaagcagga gtatgtcatt gatggacgtt cagcaactgc
    16681 attatgcgcc acttctaaat cagcttcatc agcgtgcggt gaggtcggtg gtaagtcagt
    16741 tggcattgcg gagcaagtcg ttgactcatt atctggaatc acaatatttt cagcctcccg
    16801 gtcaggaggg gtcactgctt gcagacccgg tattcgaaag tacattcggc tggatgcctg
    16861 cggatgaaac aatggaggat ttgcgggaca agctgatctc cagcccgctg gttgatgcat
    16921 tgagcaaagc gccagtgcca tttcgtcatc aacttactgc gtggcgaacg attctggaag
    16981 acaaaaaatc cttgttagtg agcagcggta cgggatccgg gaaaacagaa tgctttatgg
    17041 taccggttct ggaagatctt attcgtcagc aaaaatcgac ccgccagtca ctaacaggga
    17101 cacaagctat tttcttgtat ccgttaaatg cattaattaa cagccaacag gagcggcttc
    17161 gcgaatggac gcgcggattt aaggggaaaa tacgcttcgc gctatataat ggcgaaaccc
    17221 ggcataccaa atacgaaatt caggaagatc agttgaaagt acctgaacaa gctttgagtc
    17281 gggaagccat ttatgaagca ccaccttcca tcatggtgac gaataccacc atgctggaat
    17341 atatgctgat tcgtcgtaaa gatgccccaa tcatcgaaaa gtctcagggg atgttaaagt
    17401 atatcgtcct ggatgaagcc catagttata ttggttctca ggctgctgag ttagcgctgc
    17461 ttttacgtcg tgttatgcag gctttcaatg ttggacccgg cacagataaa cctgtgcaga
    17521 ttatcgcgac ctctgccact attggtgaag actctcctga aggtaataag gagcttgcta
    17581 aatttgtcgc tgatctcgcg ggcgttacag aacgtgatgt caaagtggtt cgcggttatc
    17641 gacaaatacc gcgggtatcg gagtcgctga ttcgtcatga ataccccaca tctttaactt
    17701 ctcttaaatc cctgagccct caggatcttt accaacagct ttgtcattac cgtgtggccc
    17761 agcaattgcg tcaggcgtta actcatcccg gacggcaggc agtacgtttg agtgagttgc
    17821 tcaatgttgc cagaagaacc tggccagata ttaatcaccg tgagctactg caactcctcg
    17881 atctaatggc aagatcgcga gaaggtgaac tggcttttac gccattacgt atgcatgggt
    17941 ttatcagaac actggcgggt ctatgggcat gttcaaacaa acaatgctca cataaagcac
    18001 atgaattaaa tcagagtgac tggccatttg gtcaggtatg gtttgagcag cgccaatatt
    18061 gtgactgtgg agcgccggta tttgaagttt tacgttgctc cgggtgtggt tcagcttatt
    18121 tgtctgccaa agaggagatg cgcggtgatg gaacgaactg gttggtagcg caaccagccg
    18181 ctgccgaagt ggatgagttt gctcttgatg tagatgttta ttctgaagat gaagacaacg
    18241 acgaattaga caataactct caatttgatc gtctgattgc gagcgatggt gaaaaatata
    18301 tcatttcact ggaagaaacg acggcgggga aaattgatag tgaagcgcaa aagcactatg
    18361 aaataaacct gttaagaccg gaatctcgag gtgagggacg caataactcc tttgcttgtg
    18421 tttgctgtgg tgacactcaa cggaaaaata atcctttatt ccgtccgctt cggctcggcg
    18481 cgcccttttt tttaaatgaa attattccta ctctgctgga gttttctcca ttaccgcaaa
    18541 caagagaaga gcagttagga ccgtttaatg gtcgacgctt gctcaccttt acggattcgc
    18601 gtcagggtac cgctcgcatt tcagcccgct tacagcagga tgccgataaa gcgacgttgc
    18661 gagctctttg ttaccaggag ctggcaaata ttacagagcc ttcagcaaaa ctcagcccgg
    18721 cacactgtgc actgttagag aaagttatta gccagttttc tgtcctccct ttaaaaaatt
    18781 ccatcgacgc gctgaaaaaa gcgatggctg cgaatgagga gcttagcccg acggacattc
    18841 aggcacttaa aattattgag ccgatggtga gttccttaag cacggagcat gctgaagcga
    18901 tgcaaatact tcaggctaac tctggcaatc agcagaagaa aaccatgtca tggcaaacgc
    18961 tgtctgaaca gctggttggc agtgtggacc ttgccgatag aatgagaggc agttttaaag
    19021 aattgtctgg gcttgatctt actaaacaac agtttgcaga tttttgttta tacagcgagt
    19081 ttggtcgccg ccctaaaaat gcctggaccc tcgaaagtct gggtctggta gccattcact
    19141 atccattcat tgataaagtt acaacctgcc cgcaggactg gaagacatta ttacccgatc
    19201 ccgataagca attagcggaa tggcgtaatt tattgaagat tactcttgat ttctttatcc
    19261 gtgaaaacag tgccgttttt tacgaaaacg agcaataccc tcgctggatg ggggcacgtt
    19321 tcccggtaaa aatgctccag gggccagata aaaaaaacaa agggcagaaa cgtgatcaac
    19381 tttggccaca aatacgcgat cgccattata accgggtcat taagctatta atcgcagtct
    19441 ttccggcaat acaacctgag caggctcact ggaaatcgtt agttaaccac ttaatgattg
    19501 aggtctggga tgcaattcga ccctgcttac gtcagtttga gtcgggttat cagctggata
    19561 ttaaacaaca agctgaattt tacagtccaa agcaagtgtg gcgttgcccc tatactcgac
    19621 gtgcgttaga cgtcacgttg ctgggatact ctccgtattt gccgggtagc aaagaaattg
    19681 cacctgaaaa agcagtgttg attgaaatgc ctgagcttcc tgtacgtcac tggcgtttgt
    19741 ctggtggtgg agaaatcgcc cgagaagaac gtctggagtg gctggaaagt aatgcgctga
    19801 ttcaacatgc ccgcgaggaa ggattatggt ctactcgcag cgaccgactg gccctgaaag
    19861 atagctggta tcgtctggag gaacactctg cgcagagaac gcctgagcag aaccagttta
    19921 atgaaaaaca atttaagtca ggcaaagtta atgtgctgaa ctgttccacg accatggaga
    19981 tgggggtgga tattggtggt atgagtttgg tggcaatgaa caacgttcct ccagcgccgg
    20041 caaattattt gcaacgtgca ggtcgcgcgg ggcgtcgtgg agagagtgca tctgctgcca
    20101 ttacgttatg taaaaacacg gcgcatggaa tggaggtatt caaagacccc ctctgggcgt
    20161 tcaacactac tgccagtgcg ccacgggttc gttttgggag cagtagtatt gttcaacgac
    20221 atgttaatgc cctggtttta ggtctgtttt tacgagctga agttcctgat gcgacaaaac
    20281 tgagttgcaa atggttcttt gaaggtgatg agagccagtg tcttcgtttt ctccattggt
    20341 taaatcacca ggccgatcag ctggcggata aacttaaacg ccttactcag ggaaccgtac
    20401 tgatgtcatt gacggcaacc cagctcttga cccgcacaca agcaatgatg cagcaggttg
    20461 atatccgctg gagaagccaa cttgcgatac tgttggaaaa tatcgaagcg ttgaaggcgg
    20521 ataacagcgc gtgggaagaa acaccagcag gtaaagccat cgcttatcag cttcgtgatt
    20581 atcgcggtgc atatctgttt tctaaactca ttagtgaagc gttcctgccg gggcatggtt
    20641 tcccggttgg cgtagtcaat ttcaattatc tgacggcaga tgagctggaa aaacgtcgcg
    20701 caataaaagc cactcaggct gaccctaatg aagggggaga aagcttttct cgtcgtattg
    20761 aaaaattgcc cagccgtgat ttgcctacag cattacgcga gtatgcgcct ggtgcagatg
    20821 tggtattggg cggaaaagta tatcgttcct cgggcattat gcttggcaag gtgcttgcca
    20881 gcgggcagga gctgtcaggc gatcatcata ttccgtggtt ctggcattgc cgtaaatgtg
    20941 gtgcgggggc aaccagtact acccatcctg tcgaatgtag tcactgcaaa gcggatatac
    21001 agcaacttga tgtgaagcgt tatttacagc ctgttggttt tgcgacagat attcgttatc
    21061 aggcacataa tgatgtatcc atgcctgcac aacttccctg gaaagatcca cgtgttcttg
    21121 taccgtcttc ggtgtgggtt tctttacccg atgctgggct aggacgttac cgttttagcc
    21181 atagtgggga attgttccac ttcagtgaag gggaatttgg gcacggctac gcaatttgcc
    21241 tctcatgtgg tcgggcagaa tctcaaactc aaccccagcg tacccctgaa aatctaaaga
    21301 acccagagag ggaaaatacg cattacctgt tgcgaggggg cagtaacgat cgtcagggga
    21361 gcaataaatt gtgccacggt catgtgcata aagatctgtg gttgggatat agcagtcgta
    21421 cagatatggt tgagctgcaa ctcaatgatg ataatggcct gttgatccgt gatgaggttg
    21481 ccgcgcgttc ccttgcggtc gctttgcgtg aagggttagc gcataaattg ggtatcgaaa
    21541 atactgagct gggcgtgacg acgcagcaag cgcgcgatat caatggctat accggatatt
    21601 ccatcttcat ttacgacaat aatgcgggtg gtgcaggtta tgcagtgcaa ttgatcgacc
    21661 attgggctga tgtctttaat tacgccagaa aattgctcga ttgcagttgc gataaattct
    21721 gtcaccactg cttgctcagc tacgacagtc agcattatgt caacagatta gatcgtcacc
    21781 atgcattaac cctgcttacc aatgtacggt tgcagcgact taacctcgcg ccagaatatc
    21841 agtacttcgg cgatggtagc cgggttgaaa ctaaccctct gagtttacgt attgcgcaat
    21901 gcctgaacag cgagatttat gactcctgct cgctggttct tgcggggccg caggaacagt
    21961 gggactttgc ccaatggcca ttatttaaag agttgctcca gtttgcctcc tccggtggga
    22021 atgttgagtt gctggtggcc acgccgctgg caaacttaac tgatagctcc agacatcagc
    22081 tttccgcgtt ggcggcgatg ccgggaggtc gcttacaagt gaaatccatt gccactgcgc
    22141 agctcatgca aggaaagggg cgctggcttg cgcaggtgac tcgcgaaggg caaagccaac
    22201 agtgggccgc agacgacagc gctaccgttg cacctggtga attatgggga caaagtgcgt
    22261 cttcaccagt agtgacactt aaaggaacgt ccggaaaaac cttctcgggt cagaccttaa
    22321 gtgcagaaga tttactgccc gccttaccca ccggggcggt gcgcatcaat ctctgtgaac
    22381 aactggacgg gccgctggaa ggttttggtt cgcgtttctg gtcgttagtc acgcaacagc
    22441 acgctggctg gaagcaggca tttacccgcc acaaagagat aacgcatgtt gagtacagcg
    22501 atcgttatct taatagccca ttcacggcgc gtttgttagg tgaaatctta accgagctgg
    22561 ttgaacaggg aatggcggaa cgcgccagcc tcactgtttg cgtcaaaaaa ttggattaca
    22621 actctcgcca acacgacgct ctctataacg cgtggcttaa tgaggaagat cgtcagcagg
    22681 ttgtgaccac tttacttgaa gaaggatatc tggggcctgc gtggccggga gcaattagct
    22741 ggttaacggg ggataaccag agtacggagc atggacgaga actgacagtc accttcagcg
    22801 atggcagtca gcattatgtc ctgctcgaca tggggcttag ctactggcgc tgtattgaag
    22861 ataccttctt tgattttgcc ctgcgagtac cgcagcaagt tgaacggctg gcaaacacgc
    22921 gagcgcgagc tgttgccccg ggtaatgacc ttaggagtta tattatcgct ggataaataa
    22981 ttgctcccct ggggatatct tccaggggag ttgacccagc tcacttttta ataatttttg
    23041 tttgaaaatc atgcgcaatt tatgcgcatt ccctcccatt ttgtcacttt gccgggggcc
    23101 ttcatgatat ctttaaggcc ctctttttta tcgcagtgtg ccgcgccaca tttttcagga
    23161 tgacgttacc gccactgccc caaatagaca gaaaaaccga tgaataagca aaattatgca
    23221 cctggtatgc gggtggttat tcgtgatgcc gaatggcgta ttcgccgggc ggatgacagt
    23281 ggtgatggtg ggtatttgct gacctgcgat ggtatttcag agcttgtgcg cggtaaagaa
    23341 gggttatttc tgaccaaact ggaacaaaaa gtagagatcc tggatcccgc aaaaacgcat
    23401 ttggtggaag atgaatccgc caattatcaa gcggcacagt tgtatattga aagtcagtta
    23461 cgccaacgcg taccgacaga cagtaaagtc cattttggtc atctggcggc gatggattcc
    23521 atgccgtttc agctcgatcc tacgcggatg gcgctggcac aaccgcgtca gcggatactc
    23581 attgccgacg ctgttggtct gggtaaaacg ctggaagccg gtattctggt ttcggaactg
    23641 atccgtcgtg ggcgcggaaa acgtattctg gttctggcgg ttaaatcgat gctgacacaa
    23701 ttccagaagg agttctggag ccgttttgcg atcccattga cccgcctgga ttctgctggt
    23761 ttacaaaagg tgcgtaaccg catcccaacc aaccacaacc cgttccacta ttttgacaag
    23821 accattatct ccatcgatac gctgaaacaa gacattgaat atcgtcacca tctggaaaat
    23881 gcctggtggg atattattgt cattgatgag gcccataacg tcgcagaacg tggaaccagt
    23941 tcgttacgca gtaaactggc aaaactgctc gccgggcgca gcgatacgct gattatgctt
    24001 tcagcaacac cgcatgatgg taaggctgaa agttttgcca gcctgatgaa catgcttgac
    24061 ccgacggcga tcgccaaccc gaaagagtat gaatacgccg attttgccga taaaaatctg
    24121 gttgtccgtc gcttcaaaaa agacgtgaaa gatcagatgt ccggcgagtt ccccgagcgc
    24181 aatattgtca aattgactcg cctggcatcc ggtgccgaag aagaagcata ccgtcgtctg
    24241 gttgagagcc agttccgtga tgatgacgat gaacaggccc agtctaacaa aggtcgtctg
    24301 ttcaagataa ccctcgaaaa agcgctattt tccagcccga tggcgtgtgc cagcgtcgtg
    24361 gcgaatcgtc tgaagcgtct tgagagccgt aaagatcata atagccagag ccagatcaac
    24421 gaactggagt cattgctgtt ggcgctgaat aacattgatg ccagccagtt tagcaaatac
    24481 cagttgctgc tcgacaccat ccgaaaagac ctcgcctgga aagccaataa cacggaagat
    24541 cgcctggtga tcttcaccga aagtattaaa acgctggagt tcctcgaaca gcaactgcga
    24601 gcggatctga aattgaaaga tgaccagatc gccacgctgc gcggcgatca gggcgatacg
    24661 gtattgatgg aaaccgtaga agcctttggt aaaacgcagt cgccgttgcg tcttctggtt
    24721 tgttcggatg tggcatctga agggatcaac ctgcatcacc ttagccacaa aatgattcac
    24781 tttgatattc cgtggtcgtt gatggtattc cagcaacgta acgggcgtat tgaccgctat
    24841 gggcaaaaac atcagcccca aatccgctat ctgcttaccg aggccagcga accacagatc
    24901 aatggcgata tgcgcgtact ggaagtgttg atcaacaaag acgaacaggc gcagaagaac
    24961 attggggaca gttcggagtt taccggcaaa tttacccagg aagaggaaga agagcaggtt
    25021 gcagagttca tgatgcagga tgatggtgcc agcctgtttg atcaactgct gaacagcaac
    25081 gtctcggaaa gcgccgaaca cgatctgttt ggcgaaatat gcagtgcggt ttccagcgat
    25141 gcctcaatgg tcaccgaaac agacaccagc ttgtttgcca gcgaacaggc gtactgcgaa
    25201 agagcattgg gttacctgaa agccagtggt caaactatcc agtatgaaac cttgcctgat
    25261 aataccttgt cgctggtggc accggaggag ttacgccgcc gcttcaacca gctaccgcct
    25321 gaaattgccc cggagaactg gcagctctat ttaagtcagg ataaaaccgt catcaccgac
    25381 gcgattgcgc gcgctcgcgg tgagcaacat gcctggcccg atgtgcagta tctctggcaa
    25441 atcaacccgg tagtgcagtg gctggacgat aaaatctctt cggcttttgg tcgtcatcag
    25501 gctccggtta tccggctgcc atacctgctt gaacccgatg aagatcactt cattctttcc
    25561 gggttattcc cgaaccgtaa atcacatccg atggtgaacc cgtggatagt ggtgagcttt
    25621 aaccgtgaat cgctaatcgg cagtcagcct ttcgctgagt ttttacaacg tcatccgcag
    25681 ttgagtaaca agctgactaa cagcggcggt aaagatcgta atcaccaacg ccagcaggat
    25741 ttactggaag cggctattgc tcatgcccgg gaggtattca tccatgatcg gaatgcgttt
    25801 gaaacgcaca ttaaccagca actgaatgag catctgcaaa agctggacgt tttgcgtggg
    25861 cggcagttga gccaacttga gctggatttt gccgataaca aacagcaatt gtcagtcaag
    25921 cagagccgta aagagcagag acaacgcgaa atcgaacaca attttgacag ctatatcgaa
    25981 tggattgagg acaccatgac gactgaaaaa gaaccctaca ttcaggtaat tgctgttatc
    26041 accggagcgg agggttaatc atggcgctgg tcggtattaa taacgaaaac gaattttact
    26101 ctaaccacta tttgggtgag gttttcacca gtgatatccg cgatgtgctg gaaccctgga
    26161 tagcccagga aaatgcagcg cgtgaagcgg agcgtgccgc tcgtgaacag ggcaaagacg
    26221 tggagccggg ataccgcgct ccgtggaacc agtttaacag tctggcgact gagtttttcc
    26281 gcaaacttgc cgagcacgaa aaacagcgtc agatcccgca gcgtctggcc gatcaacgta
    26341 atcgctggca gccattgtta aaggcgctgg gctacgaaat tacgccacag atccaaatgc
    26401 tggatgacga tacaccgctg ccggtactgg cgcgttacaa cagcactgac ggtagcccgt
    26461 ggctgtggat tgttgaagca cacgatcagg aagaaggaac gctggatccg ctggcgctct
    26521 ccttactgac cgcgcaattc ccggcggata ccgacaaaca taagcacgac agcctgcgca
    26581 aaaaagccaa cggtgaatat cgcagctggc aggatttgct ctctacggcg gtcttcaccc
    26641 aaaatgaacc gccgcgtttt gtgctgctgc tcggtaaccg tcagctattg ttgttggacc
    26701 gtactaagtg ggcgcaaaac cgtctgctac gttttgattt tgaagagatt ttaagccgtc
    26761 gtgaaacgga tacgctgaaa gcgactgcgg tgttgttaca taaagattct ctgctgccgg
    26821 gcagtggggc accttatctt gactcgctgg atgacaattc gcacaaacat gcgtttggtg
    26881 tttcggaaga tctgaaatat gccctgcgcg aaagcattga gttgctgggc aacgaagcga
    26941 tgcattatct gatcgaccgt ggcctggcaa actatacagg taatcgtgcg gtggacccgg
    27001 atgaactgag ccgcgaatgt ctgcgttaca tgtaccgcct gctgttcctg ttctacattg
    27061 aagcgcgccc ggagctgggt tatgcgccaa tgaccgccaa aacctatctg caaggttaca
    27121 gcctggaaac gttgcgcgat ctggagatga tcccgctgac cagcgaagaa gatcgcaacg
    27181 ggcgctactt ccacgacagc ctgaatatgc tgtttaaact ggtgcgcgaa ggctacaacg
    27241 gcggcgtgaa aatgcagagt gacctggaga gcggcgaccg gatcaccatc catagtcatc
    27301 agttcagcgt cccgcgtctg gaaagtcatc tgtttgatgc caacaacacg cgcattctta
    27361 accgcgtggt attccgtaac gaaaccctgc aacagattat ccaggcgatg tcgttaagcc
    27421 gcccggccaa agggcgcttt aaccgccgcg gacgtatttc ttatcgccag ttgggtatca
    27481 accagttggg tgcggtgtat gaggcgctgc tctcctatcg cggattcttc gccagcgaag
    27541 atctctacga ggtgaagaaa gccggggaag agtttaacga gctggagacg ggttacttcg
    27601 tcagtaagga tgagattagc aaataccacg aagacgagaa ggtctacgag aaagacggca
    27661 gtctgcgcat tcaccgcaaa ggcagcttta tctaccgtat ggccgggcgc gaccgtgaga
    27721 aatctgcctc ttattacacc ccggaagtgc tgacccgctc actggttaaa tatgccctga
    27781 aagaactgtt taaggagcag attgatccca ttagcgatcc gcacgccaaa gctgatgcca
    27841 tcttaaacct caccgtgtgc gaaccggcga tgggcagcgc ggcgttcctt aacgaagcca
    27901 tcaaccagct ggcggaagcg tatctgttcc acaagcagca ggcggaaggt cgccgtattc
    27961 cgcaggatcg ttacacccag gagttacagc gggtgaaaat gtacattgcc gacaacaacg
    28021 ttttcggcgt ggacttaaac ccggtggcgg tggaactggc ggaagtgtcg ctgtggctga
    28081 acgccattag tggcgatgcg tttgtaccgt ggtttggtta ccagctgcac tgcggtaact
    28141 cgctggtggg cgcgcgccgt caggtgttca acaagagcga actgacctac aaaaaagcca
    28201 aagatccgag ctggcttaac agcgagccgg tcgaactggc gatgaacacg ccgcgtgaag
    28261 agacgcagat tttccacttc ctgctgcccg acggcggtat ggctaactac agcgataaaa
    28321 ctgttaagca gcgttatccg gatgacttca aagcgctgga cagctggcgc aaagagttta
    28381 ttaaaagctt tgccgggcat gagattgctg atgtgcagcg tatcagcgaa aaggtggaag
    28441 cactgtggaa cacctatcgc cagcaactta aagcagaacg tctgaaaacc gccgacagct
    28501 acccggtgtg gccggcagaa aacagcgagc agacgcgttc ttcgctgagc agtaaagatg
    28561 aaacctttag cggtcgtctt gaagataaca gcgcctacca gaagctgcgt tgggtaatgg
    28621 actactggtg cgcgctgtgg ttctggccga tcgacaaagc cgatgagcta ccggatcgcg
    28681 gcacctggtt gtttgagatt gaaaccctgc ttgacgggat tgtaatcacg gaaaaagtca
    28741 ctgaagttgc ggagcacacc accggcgatc tgtttgccga agaaggcctg ctgcgagaag
    28801 agtcttcgct gttttctgtt gctggtcgtc tgaaaaccga ggtgttgttc cgtcatttgc
    28861 cgcgtctggc gattgtcgat gccctgagaa agcagcaccg tttcttccac tgggatctgg
    28921 agttctgcga cctgtttgcc gagcgcggcg gttttgacct gatgctcgga aacccgccgt
    28981 ggctgaaagt ggaatggcag gaagcgggcg tgctgggtga ttacgagccg gaatttgtgc
    29041 tgcgtaagct gagcgcctcg aagctggcaa cgttgcgtat tgataccttt aaccagatcc
    29101 cggcgctgga agcggcctgg cgcagcgagt atgaaggctg tgaagggatg caaaacttcc
    29161 tgaatgcgca gcagaactac ccggtactgc gcggggtgca gaccaacttg tataaatgct
    29221 ttctgccgca ggcctggcga ttaggggcgc agaaaggcgt ggcaggtttc ctgcacccgg
    29281 aagggattta tgatgacccg aaaggcgggc aattacgtgc ggcggtatat ccgaggctga
    29341 gggcgcattt tcagtttcag aatgagttaa atttgtttgt tgaagttgat caccatgcga
    29401 agtttagcag caatatttat tctgctagcc ctagcacagt gggatttgaa catatatcta
    29461 atttgtatgc tccgcaaact attgatgcat gttttgaaca ttctggcagt ggggacattc
    29521 ccggtctcaa agacgagatt gagagcgagg gaaaattaaa agttgtatgg aacacatctg
    29581 gccaccgttc tcgattaata agtatcgcca ctcatgagct agaattattt gctcgtctat
    29641 atgacagcga aggaacgcca gcctggcagg cacgtttgcc agccttacat gctaaacaac
    29701 ttgttgctgt actggaaaag tttgctaatc agccgaatag attaggtgat ttgcaggggc
    29761 agtatttttc aacggttatg ttcgatgaaa catatgctca gagggatggg acaattttac
    29821 ggcagactca attccctcaa gattcatcac aatgggtact gtctggccct catttctttg
    29881 ttgggacgcc gttctacaag actccgcgcg aaaactgtac gcttaacagc gattatgact
    29941 gcctggactt gctaactctg cctgacgact atctgccgcg cactaactac attccggcat
    30001 gtgatgcaca ggagtatgca aaacgtactc catgcgttac atggactgaa ctggctgaag
    30061 atgaaccgaa gaaggtaaca gattattatc gcttagctat cagagccatg ttggctcaat
    30121 cgggggaacg tacactaatt agtgctattt atccgccaga aataagtcac atgaacgcag
    30181 tacgttctta ctgctatagc tcacagaatc tgttactcga acattcaggt atgtgttttt
    30241 ctttaccttt tgattttatt tgtaaatcta ctggcaaggc aaacttacat cagatgcttg
    30301 atggtttctc atacgtatta ttcaatccga gacaaaaggc attattatac tgcttagtat
    30361 tatcattaaa ttctgtaaat gatgtatatg ctggcctttg gcaatcctgc tacaccccag
    30421 acttcaacac ccagcgttgg agccgcgatc tcccgcagct cccccaggat ttcttcgcca
    30481 aactgacccc agagtggcag cgtaactgcg ctttacgctc tgactacagt cgtcgtcagg
    30541 cgctggtgga aatcgacgta ttggtggcgc aggcgctggg gttaactctc gaagagctgc
    30601 ttaccattta tcgcgttcag ttcccggtga tgcgccagta cgaagcggat acctggtacg
    30661 atcaaaacgg tcgcattatc tttaccccaa gcaaagggct ggtgggcgtt ggcttgcctc
    30721 gcaccgcgcg taaagctgac ctgaaaaacg gctttgtctt taacgtcgac agcccggagt
    30781 ggaccggcgg tgactgcacc gatcaagcta tcggttggga tgatgtcaaa catcttaaaa
    30841 ccggtaccgt cagcgtcacc tttgatgatt atacccgcag cgacgaaggt gagcgccgta
    30901 ccgtcacctg gcaggctccg tttatcaagc cagatcgcga agatgactac aaagtggcct
    30961 gggcgttctt tgcacaagat aaggagagcg cctgatgttg ccgtccgttg tcagtcggca
    31021 ggtcgccgac agcgttgccg cctttttacg ggcggcgttc ccgctaaaca gcccgctgtt
    31081 taacggtgag aataacgata atgtctcaat gctggagcaa tttctggccc agccggaaac
    31141 gctgctgaaa ggaccatacc tttccgccca gttgccgttt cgtaaaagtg acttaccgct
    31201 gaattttttc cctaatctga cgctgccgtt cccgccgcac gcgcatcagg cccgggcatt
    31261 tcagcgtctg ggaagcgacg cgccacagcc gacgctggtc gcaaccggta ccggctccgg
    31321 taaaacagaa tgttttatgt tcccgctgct caaccactgc gcgggcgcgt cacaagccgg
    31381 ggttaaagcg attatcatct acccgatgaa cgccctggca accgatcagg ccagccgttt
    31441 cgccaaaacc atcgccagcg atccacaact gcatggcaag gtgaccgtcg gcctgtttgt
    31501 cggcgacagc gaaatcgaac ccagcaaaaa aatgtcggca gaaaaggtca ttacctgtaa
    31561 gcacacgctg cgcgaaagcc cgccggacat cctgttgacc aactacaaaa tgctcgacta
    31621 cctgctgatg cgccctggcg atcagccgct ctggcgttac aaccagcccg gttcattgcg
    31681 ttacctggtc gtggatgaat tgcatacgtt tgatggcgcg caagggtcgg atctggcctg
    31741 tctggtgcga cgtttgaagc atcacatagg tgtggataac caacgattcg cctgcgtcgg
    31801 gacatcagct accgtcggcg acgaactggg gcaactgctc gattacgcaa aaacaatctt
    31861 tgaccagccg tttaccgatg acgccgttat tcgcgaagat cgctacacgg cagcggagta
    31921 cttgcagaat tacgccattg cgtacagtca gtatccgggg ccggaggtcg cttcggccct
    31981 ggacccgcag agttacgcta ccccggtagc gtatcttaac gggcaaatcc ctttgtggtt
    32041 ccccgacagc gacttgcaac tcccggacga tctggagagc gacgacgggc gtgaaaagcg
    32101 tattgcgctg gggtcactgt tacgccgcca cagcgtgatg catgtcttac tccatgatct
    32161 gcaaggcggg atcctctctg aacgagagtg tctggaaaat ctccaggtca tgctggcaga
    32221 aagcgccgac catgcccgac gcgtgctgca aagcattctg gcactgatcg cgcaggcacg
    32281 tctcgaagta ccggaaacgg aacacgatcg gcaaaaacgt ctccaggcga agaaagcgcg
    32341 tccggtgctg ccgtttgtgc aactgcgctc ccagttgtgg cttcgtgaga tgcgtcgtct
    32401 ggtcgccagc gtggcgaaaa cgccccgact ggtgtttgcc gatgatgtgg cggtggaaga
    32461 tcgcgaacac tatttgccgg ttatccactg ccgtgactgc cacgcaactg gctgggccag
    32521 cctgcgtcat gggcaaagcg ttcagttgga aacagacctc gacggcattt atcgccagtt
    32581 ctttgaaaaa gggcgctcca tcgtccttgc tttcccggat aacaacgaaa aagcggtcag
    32641 tggcgcacac cagaaactgt gtcctacctg tctgaaactg aaccgacagt ccagcgtcca
    32701 gtgtggacat tgcggtcata cggaactgtt gcaggtattg atcccggata tgctcaaaga
    32761 gcgtaaggac gagctggagt ttgctaatga atgcccgtac tgcaacagca aacaggggat
    32821 ttcgattctg gggtcacggg ctgccagtct gtcggcggtg atgattcacc agctttatgg
    32881 cagccagttc aacgaacata aaaagctgat tacgttttct gactcggtac aagacgccgc
    32941 gcatcgtgcc agcttcttta ctgcccgtac ctggccgctg atgattcgtg ggcaaatcgc
    33001 cagtgttctt agcgaaaaag agacattacc gctggatgcg ttcgcggcgc gtgtttgcca
    33061 acaaacgcgt gaacgttcac cggacgatgc tcattttgca gcaaacgtca tcgcaccaaa
    33121 tatgcagtgg ctgaatgatt atcagacgct gaaaaatgaa ggcaaactgg cggagaactc
    33181 cgatctgccg gagctggttt ccctgcgcct cgactgggag gtgtacagcg aatttaccct
    33241 gcgcgcgcgt atcggacgta cgctggaagc caccggttat gccgttgccg gagtggataa
    33301 agatcgcttc aatccggcaa ttaccgccct gcatcaacag ctttgtgaag agctgggtga
    33361 tgagatgtct ggtgtgagcg tcgagcaggt agcgcacctg gcgctggggg ttatctggta
    33421 tcaacgccag gccggggccg ttatgcatcc ggcgttcgaa agttatcgcc gtgatggcac
    33481 gacctttatg atgacgcaaa aagagcgtac ctcgcgctat atgccttcta tcggtccgaa
    33541 aacgcgtaaa ccggcttatg cgtcgtttga aaaaattaat ggtttccatc gcctgattgg
    33601 cgcgaagtct aaccccagtt ggtatcagca ttggatcaat cgcacgctga gtaacggcaa
    33661 taatctgttt atcagtagcg ttgcggaaac agtgttacga cgcctgttta cggcgttgaa
    33721 aatagccgga gtagtaaaag attttgacac caaagggcgt gaagcctggg gactggtgcc
    33781 ttcggcgctg gtggtgagcc gtgacgtcgt tcagcttaat tgctcatgtt gccgtgaggt
    33841 tgtccgggct ccggccgatc aaggctggca gtggcgtaat gcgccttgtc tctctttacg
    33901 ttgtgaaggt cgttaccagg aagtcgacaa tacggcgacc tggcgttggg agaacatgga
    33961 catcgcgcgc gtacagggcg cagaacacac ggggctgctt agccgtgaag atcgcgaagt
    34021 caccgaaaaa tcgttctacc gcggcaacca accgtggaat atcaacctgt tgtcggcaac
    34081 accgacgctg gagatgggta ttgacgttgg ggatctttcc accgttttac tctgttctgt
    34141 cccacctgct caggccaact acttgcagcg tattggtcgc gccggtcgta aagatggtaa
    34201 cgcgttaaac attaccgtgg cagaagggaa cccgcacgat cagtttttct ttgaagaacc
    34261 actggagatg atgcagggcc aggtgcaggc tcctggcgtc ttccttaatg caacggctat
    34321 tctcgaacgc cagctggcag ctttttgtat ggataactgg gtgaaaaccg gtgtaccaga
    34381 atccgctatc tgtaaaaacg tcaaacagat gctggatgag ctggagttcg ggcgtaaatc
    34441 gggttttccg tataacttac tgcgatatat cgaacagcat catgcggaga ttgctgcgca
    34501 atttacggca atcttccccg atcttgccgc agaaactcgc caacagctgt tgtcttatct
    34561 tcagggcgcg ccaggacaac gctcgctggt gcagcgtatc gaagaagcgt taaaactgct
    34621 ggtggaagat cgcaaatctt tgcgctcacg catcgataaa ctcaagcgca gtatcgataa
    34681 actggagaac gcgccgcgcg atcaaaactt tgatagcgat atgcgcgagt taacttcgga
    34741 acggcaggcg ctgatggcgc tggtcaatca aatcaacaat aagcaaacgc tgaatttcct
    34801 cacggatgaa ggtctgctgc ctaactacgc cttcccggaa gccggtatta cgttacgttc
    34861 tgtgctctgg cggcgtaaag aaggcgggga agcacgtgaa tatcaaaaca ccactttcga
    34921 atatgaacgc ccggcatcaa cggcgctggc tgagctggct ccgctgaata atttctatgc
    34981 cggtgggcat aaagttgaaa ttgagcagat agacctgaag gtctctgagc cggagaactg
    35041 gcgtatctgt agccactgta actacagcga aaatatcgac cagacgggcg accagcataa
    35101 atattgcccg aaatgtggca caccggggtg ggccgatgcc ggacaaaaaa ccacgctgct
    35161 gaaacttcgc caggtttatg cccgcgccag cgcacgtgat agccagatca gcgacgagag
    35221 cgacagccgt gaacctgctt tcttccagcg tcagctattg gttagtttcg aaaaagaaga
    35281 tgtatcggcg gcatacgcga ttgaggaagg cgaagttccg tttggttttg aatttttaag
    35341 caaagtcacc ctgcgggata tcaacttcgg caaaatggcg gatgatgcca atgagttgat
    35401 gattgccgga gaagcgaaga agcgaaccgg gtttaaagtt tgcctcgggt gcggcatggt
    35461 gcagcgtcct cgcgatcatg aaccacgtca tgatttgagc tgcaaatatc gcacggaacc
    35521 ggaaaaggcg aagttcgaag attatctcta cctctatcgc cagctggagt cagaagcgtt
    35581 gcgtattctg ctcccggtaa gcagttacag taatgaccgt gtcgtggagg cttctcttgg
    35641 ggcggcgatc cagctcggtc ttaaacatta cttcaagggg aatgtcgatc acctgaaagg
    35701 cgttgtgtac cgcgagccgg aaaatgaagg cgaatcctgg cggcagtatc tggtgattta
    35761 cgataccgtc ccggggggga ctggttctct taaagaactg atgcgcactc ccgacaattt
    35821 gctgaaatta cttgaactgg cctatcaggc aatagtggaa tgcagttgca acaatgatac
    35881 gcataaagat ggttgttatc gttgcgttta cgcttaccgt gaccgtgggc gcatgaagta
    35941 tgtctcccgc gatcaggcgc gtctcttgct ggcgaaaatt ttgcaggcaa gagattcgat
    36001 acgggtaatt gattccatca aaaacatttc cctcgaagcc atgatgggca gtgagctgga
    36061 aaaacggttt attagctgcc tgcaagaaaa taaaaacctg attgtcagtc gcagttacgc
    36121 gcatcagggc gctgggtgga ttattaacac ccgcagcgaa ccgatgatga gctggcatct
    36181 taaagctcag gtggatttag gcgctaaaga ggcggtgggg atcccttgtc gcccggatta
    36241 cgtgctatat ccgcttatgc agtctgatgc cattaaaccg gtggctattt tccttgatgg
    36301 ctttgcgttc cacaaaaata gcgtcgcaga ggatgtgcaa aaacgccagg cgattcggga
    36361 tagcggtaat ttctgggtgt ggacagtcac gtgggccgat ctcaaagagc cagggcttaa
    36421 acatgttcag gatgtcctgg gacttgggca taacccggat atgaaacagc ctaagtttta
    36481 taacctgttt catgatacca actttgcggc ccttgaagcg tcgttccggg aacgcaacag
    36541 ttttgcactg ctgctggatt atctcgccga tccgggcgga agaacgcagt tatggcagag
    36601 aatggctgct gcacatgcct gggtgtggtt ggatgtgaag aagtcacagg acgcgggaac
    36661 gaaacaaaag tatgcctatg aaatgcagga aaacgcgcca gcttggcggc tagcagagct
    36721 tttgccagac gaaccgtttg tctttggtgg tttactcgat agctgtaact catcgcagca
    36781 atttattgag ctggcttcgg tgttaccaca acaagccatt aaaccgacga ccagcgtcgc
    36841 acaaatgcgc agctggctgc gtttgcatat ctgttttgac gatcgctata cgcaggatga
    36901 cggttatgaa gcgggactca atggcttctg gcgactggta aacctgttac agttcctgcc
    36961 ggatatgacc tttaccagcc gtaaagccgt gcatttaccg caggaaagcg ttgacactaa
    37021 agtttcggca acgccagagg cagtaccaga gactgatgag agttgggctg agattattga
    37081 gtttggctta ttaagcgctg aagagatcgc gttgcttcag tctcattctc tctctgcgcc
    37141 gactctcggc tacgaattgc aaaatgatga aggtgaaatt attgcggaag ccgatctcgc
    37201 ctggccatca caaaaatggg cattgattat tgataatcaa gaatttattc cattattcac
    37261 aaccaggggt tggcacgttg cattcgggcc aattgacgaa aatacattgc aacatctgtc
    37321 cggaggtgac aaatgacggt gcaaacgccc aaggtggcac tttctgatgg ttttctgggc
    37381 gcattcgcgc ggatcccaaa ggcacagcaa aaaaaggtgc aggaattcat ctccaaattc
    37441 cgtcaggacc cgaccagtaa tggacttaac tatgaaaaga tccacgatgc gcgcagtaaa
    37501 aatgtccatt cggtgcgaat cgatcaaact tatcgcggca ttgttctgaa acctgaacag
    37561 ggtgccctgt atatgctgat gtgggtggat aaacacgacg aagcatatga ctgggcgcgg
    37621 cggcatgatt gttcaattca cccggttacc ggcgcgattc aggtcattga tattagctat
    37681 atcaaaccgg cagcggaaac agtggttgat aaaccgaaac tcttcgctgc ttacagcgcg
    37741 gaacagattc tggctctggg tgtgccgcct gtctttattg atcaggtgat ggcgcttacc
    37801 gatgaagccg ggcttaatca acttgaaagc ataatgccag cagaagcctg ggaacctctg
    37861 cactggctgg cagaagggct ggattaccag gaagtgctgg aagagtttaa cgatcatcgt
    37921 gatgagcctg ttgataccaa cgactttatg gaggcgattg aacgaagtaa acgtcgcttc
    37981 catgtcgttg aaaatgaaca ggagctgttg cagatgctta atgcgccttt agaaaaatgg
    38041 cgtgtatttc tgcatccgag ccagcgaaaa ttggtggagt cgcccgctaa tggtccggta
    38101 cgagtactgg gcggtgcagg tacagggaaa acggttgttg cgatgcaccg tgcgcggtgg
    38161 ttatctcagc gtctggcaga taaacccggt aagaaagtac ttttcacgac ctttacgcgt
    38221 aacctggcgg cagatattcg cgctaatctc cagcggttgt gtacgcgaga agagatggcg
    38281 cgtattgacg tcgttaacat tgatgcctgg atgagcgacc aactaaaacg ccacggttat
    38341 gatttccgcg tggtatacga cagcgatgaa gggcgacgca aatgctggaa ctatgccttg
    38401 cagcaggcac ctgccgaact ggggctacct gataatttct atgctgaaga gtggcaacgc
    38461 gttgtccagc caaatgcggt ttacacccgg gaggaatacc tcaaagtcag ccgggttgga
    38521 cgtggtacag cgttatcccg tattcaacgg gcgaaaatct ggccagtctt tgaagaattc
    38581 agagcacaaa tggcgcgtgc caaactgcgc gaaatgggcg atgccatgca tgaggctatt
    38641 gttctgttta aagaaaagca ggtccaacta ccttatagca gtattgtggt cgatgaagct
    38701 caagatatcg gtgccccggc atttactctt attcgcagcc tggttcccga gtcgcccaac
    38761 gatctgttta ttgtcgggga tgggcatcag cgtatttatc gcaataaggt cgttcttggt
    38821 cagtgtggta ttaacgtccg tggtcgtcgc agtaaaaaat tgaagattaa ctatcggacc
    38881 acagaagaga cgcgtcaatt tgctgttggg ctgttaactg gtgtgaaggt tgataacctc
    38941 gatggcgaag cggataccag taatgattat ttgtcattac tgcacgggga aaagcccatg
    39001 attacgcatg cggcagattt caaagaagag gctgcaacgc tcgtcaagca aattcaggca
    39061 ttgctggcga atcaggttcg ttctcaggat atctgcatta ctgcgcgtac taaacatgct
    39121 tgtgaccgct acgcatcagc actgaatgac gccggaattg agacatttaa tttaggcaat
    39181 gatagcggtg acagcgatgc acgccctggc gtgcgggttg caacgatgca ccgtattaaa
    39241 gggcttgagt tccagtatgt gttcctggtg gggattaacg aaggcgttgt gccggaaata
    39301 aaagcattag catccgatga tcccgttgaa cagcgtgatg cattatttaa tgagcgcgcg
    39361 ttactgcatg tggctgcaac ccgtgccgta aaagggctgt ttgtttcatc atctggtgtc
    39421 cctagtccgc tattagtggc tgactgataa tatgtccaaa acaggccagg agccgaaacg
    39481 ctcctggtct atataaccgt tctggcaact ggcgatattt atattgctta gctcccaaca
    39541 gccagacaat atcaacttag aaattatatt cgcttatgcg gtttgcatat tgcacagtac
    39601 aacctgtttt ctctatatct aacgtaacgt caggatgtgg atgatttttg gatggggtga
    39661 cacactggac aaatatagct ggcacgaaaa ggctggcatt tccagctgac caatagtctc
    39721 tggaaccatg atg
//
LOCUS       DAZFAB010000033        39664 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZFAB010000033 DAZFAB010000000
VERSION     DAZFAB010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.033"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            656..1915
                     /gene="codB"
                     /locus_tag="ACHJWA_002016"
     CDS             656..1915
                     /gene="codB"
                     /locus_tag="ACHJWA_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFO4308614.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSLIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEANSVE"
     gene            1905..3188
                     /gene="codA"
                     /locus_tag="ACHJWA_002017"
     CDS             1905..3188
                     /gene="codA"
                     /locus_tag="ACHJWA_002017"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HFO4308615.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKLEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYSIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(3321..4220)
                     /gene="cynR"
                     /locus_tag="ACHJWA_002018"
     CDS             complement(3321..4220)
                     /gene="cynR"
                     /locus_tag="ACHJWA_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HFO4308616.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIHLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHLPELEAIPL
                     LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAIPLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALEECADVGENESR"
     gene            4330..4989
                     /gene="cynT"
                     /locus_tag="ACHJWA_002019"
     CDS             4330..4989
                     /gene="cynT"
                     /locus_tag="ACHJWA_002019"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HFO4308617.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            5020..5490
                     /gene="cynS"
                     /locus_tag="ACHJWA_002020"
     CDS             5020..5490
                     /gene="cynS"
                     /locus_tag="ACHJWA_002020"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009439 - cyanate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HFO4308618.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKALSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARQVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            5496..6677
                     /gene="cynX"
                     /locus_tag="ACHJWA_002021"
     CDS             5496..6677
                     /gene="cynX"
                     /locus_tag="ACHJWA_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HFO4308619.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSALLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAALTPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSTREVASSHKTTTIPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LALQLVGFCGFIWLPLQLPVLWAMVCGLGLGGAFPLCLLLALDHSVQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPARFPQLWVKE
                     A"
     gene            complement(6780..7391)
                     /gene="lacA"
                     /locus_tag="ACHJWA_002022"
     CDS             complement(6780..7391)
                     /gene="lacA"
                     /locus_tag="ACHJWA_002022"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HFO4308620.1"
                     /translation="MNMSMTERIKAGKLFTDMCEGLPEKRLRRKTLMYEFNHSHPSEV
                     DKRESLIKEMFATVGENAWVEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSIVTKDIPPNVVAAGVPCRVIREINDRDKQYYFRDYKVESSV"
     gene            complement(7457..8710)
                     /gene="lacY"
                     /locus_tag="ACHJWA_002023"
     CDS             complement(7457..8710)
                     /gene="lacY"
                     /locus_tag="ACHJWA_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HFO4308621.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAILLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLLLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            complement(8762..11836)
                     /gene="lacZ"
                     /locus_tag="ACHJWA_002024"
     CDS             complement(8762..11836)
                     /gene="lacZ"
                     /locus_tag="ACHJWA_002024"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFO4308622.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TNRPSQQLRSLNGEWQFVWFPAPEAVPESWLECDLPDADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLLSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATLFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLGLNVENPKLWSAEIPNIYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTSATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGEMRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDLVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGRTIEVTSEYLFRHSDNELLHWTVALDGKPLASGEVPLDVAPQGKQVIE
                     LPELPRLESTGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPSAPHAIPQ
                     LTTSETDFCIELDNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTVHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            complement(11959..13041)
                     /gene="lacI"
                     /locus_tag="ACHJWA_002025"
     CDS             complement(11959..13041)
                     /gene="lacI"
                     /locus_tag="ACHJWA_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFO4308623.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPKT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            complement(13078..14031)
                     /locus_tag="ACHJWA_002026"
     CDS             complement(13078..14031)
                     /locus_tag="ACHJWA_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HFO4308624.1"
                     /translation="MRPLTVAIIAVAGFSPFHLSVPFIVFSEKMAEKKRFHVIICAEK
                     PGNVDSADGFSVTATHDYTAVIQADIVIIPYWGTITQKPPQKLLEALTTARDNGAQIV
                     GLCLGTFVLGYAGLLKNKRAATHWEFEREFQARFPQTHLDINALYVDDDGIITSAGTA
                     AALDCCLYIVRQHFGSDYANHIARRMVVPPYRTGGQAQFIEQPVPKNTHDERINLLLD
                     YLRQNIAQQHDLDSLAQRVMMSRRTLTRHFMKATGSSIAEWLITERLRRSQELLGSSQ
                     LPVERIAAEVGFLSPVTWRQHFKSHFGVSPAEWRKTFRGMA"
     gene            complement(14051..14827)
                     /locus_tag="ACHJWA_002027"
     CDS             complement(14051..14827)
                     /locus_tag="ACHJWA_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFO4308625.1"
                     /translation="MKITQIRNATQIIQYAGKTFLIDPLLAPKGSYPGFAGTARAEIR
                     NPTVELPCDLTTLLNVDALIVTHLHEDHWDETASRVVPKDKLIYVQNEQDAESLNKQG
                     FTHLTVLTDETKFGDITLHKTTGQHGSDRTYADPAMAKRLGAACGVVFQHPTEKTLYL
                     AGDTLWRDDVEATMLTFQPEVVVLNAGFAHVLGHGAIIMGAEDILKTHFTLPEAQIIA
                     THMEAVNHCLLTRDALKEYARDNQIIEFVNVPEDGETLTF"
     gene            complement(14942..15775)
                     /gene="mhpR"
                     /locus_tag="ACHJWA_002028"
     CDS             complement(14942..15775)
                     /gene="mhpR"
                     /locus_tag="ACHJWA_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFO4308626.1"
                     /translation="MQNNEQTEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQE
                     RKELIEMLASRPGDDYQLAREPLKLEAILARARKEGYGQNYRGWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     R"
     gene            15966..17630
                     /gene="mhpA"
                     /locus_tag="ACHJWA_002029"
     CDS             15966..17630
                     /gene="mhpA"
                     /locus_tag="ACHJWA_002029"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFO4308627.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVDNVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGVSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     LREIVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALSTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGKTLNKLASVMTLTRPDADVSVEKVA"
     gene            17632..18576
                     /gene="mhpB"
                     /locus_tag="ACHJWA_002030"
     CDS             17632..18576
                     /gene="mhpB"
                     /locus_tag="ACHJWA_002030"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFO4308628.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            18594..19460
                     /gene="mhpC"
                     /locus_tag="ACHJWA_002031"
     CDS             18594..19460
                     /gene="mhpC"
                     /locus_tag="ACHJWA_002031"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308431.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFO4308629.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARA"
     gene            19470..20279
                     /gene="mhpD"
                     /locus_tag="ACHJWA_002032"
     CDS             19470..20279
                     /gene="mhpD"
                     /locus_tag="ACHJWA_002032"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFO4308630.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQYDVAQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAAAFSSAAPKGSLS"
     gene            20276..21226
                     /gene="mhpF"
                     /locus_tag="ACHJWA_002033"
     CDS             20276..21226
                     /gene="mhpF"
                     /locus_tag="ACHJWA_002033"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFO4308631.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHDQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEENVDQLNVNMVTCGGQATIPMVAAVSRVVRVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            21223..22236
                     /gene="mhpE"
                     /locus_tag="ACHJWA_002034"
     CDS             21223..22236
                     /gene="mhpE"
                     /locus_tag="ACHJWA_002034"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HFO4308632.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAVDKLGWQHGADLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            22412..23623
                     /gene="mhpT"
                     /locus_tag="ACHJWA_002035"
     CDS             22412..23623
                     /gene="mhpT"
                     /locus_tag="ACHJWA_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFO4308633.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRLRMQPCADA"
     gene            23725..24264
                     /gene="yaiL"
                     /locus_tag="ACHJWA_002036"
     CDS             23725..24264
                     /gene="yaiL"
                     /locus_tag="ACHJWA_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFO4308634.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(24389..25222)
                     /gene="fghA"
                     /locus_tag="ACHJWA_002037"
     CDS             complement(24389..25222)
                     /gene="fghA"
                     /locus_tag="ACHJWA_002037"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO4308635.1"
                     /translation="MELIEKHASFGGWQNVYRHYSASLKCEMNVGVYLPPKAAGEKLP
                     VLYWLSGLTCNEQNFITKSGVQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMNHFPATAKKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKEAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTQNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(25315..26424)
                     /gene="frmA"
                     /locus_tag="ACHJWA_002038"
     CDS             complement(25315..26424)
                     /gene="frmA"
                     /locus_tag="ACHJWA_002038"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFO4308636.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            complement(26459..26734)
                     /gene="frmR"
                     /locus_tag="ACHJWA_002039"
     CDS             complement(26459..26734)
                     /gene="frmR"
                     /locus_tag="ACHJWA_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFO4308637.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            complement(26894..27940)
                     /gene="fbpC"
                     /locus_tag="ACHJWA_002040"
     CDS             complement(26894..27940)
                     /gene="fbpC"
                     /locus_tag="ACHJWA_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric ABC transporter ATP-binding protein"
                     /protein_id="HFO4308638.1"
                     /translation="MSQKNFVELRNVTKRFGSNTVIDNINLTIPQGQMVTLLGPSGCG
                     KTTILRLVAGLEKPSEGQIFIDGEDVTHRSIQQRDICMVFQSYALFPHMSLGENVGYG
                     LKMLGVSRSEVKQRVKEALAMVDLEGFEDRYVDQISGGQQQRVALARALILKPKVLLF
                     DEPLSNLDANLRRSMRDKIRELQKQFNITSLYVTHDQSEAFAVSDTVLVMNKGHIMQI
                     GSPQDLYRQPASRFMASFMGDANLFPANFSEEYVDIYGYRLPRAAHFPVQGSGTVGVR
                     PEAITLSNHGEESQRCVIRHVAYMGPQYEVTVEWHGQEILLQVNATRLQPDIGEHYYL
                     EIHPYGMFVLADAA"
     gene            complement(27952..30030)
                     /locus_tag="ACHJWA_002041"
     CDS             complement(27952..30030)
                     /locus_tag="ACHJWA_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4308639.1"
                     /translation="MSQTLALHPVKKRDAIFLWVLLGWLAFVLLPSWSLDYGLLESTS
                     DEILGAYGWSHFNISWLWYLLPSLLLVRPFRQATREQRSRHYFDAGWALLCMAFIVIS
                     ATVAGRGLGYAAIVLFVELGAIMTLALTRLEWLGGDRFVIGSLIAIVALIGIFIVWPS
                     IAIFIPMFTNDAGELAPLAFMTVLSQAHIVQVIVNSIVLSIAVGIGCAFFGLVLAIYT
                     TRIAKRSAIIGRIFSILPIVTPPFVVGLGVTLMMGRSGYVTEFMVEWFGLTNTNWLYG
                     FTGIWLAQVLAFTPMAFMILDGAIKTIHPSLEEASYTLRASRWQTFNGVFVPLLKPAL
                     ANAFLIVVVQSLADFSNPLVLGGNFDVLATQIYFYITGSQLDYQAASTLGAFLLLFSL
                     LVFCIQYMWIGKRSYVTVSGKSYRGDVQPLPVTLVWSVVAILAIWIAFNALLYGSIFY
                     GSFTVNWGVDYTLTLDNFIKLFGQGMSDGAWPSLLDTLLYAGIAAPITAIFGLLIAWV
                     VVRQQFKGKKTIEFTTMLCFAVPGTVAGVSYILAFNSAPVYLTGTAAIVIISMVMRNV
                     PVGIRAGIAGLGQIDKSLDEASLSLRAGSLRTITHILLPLLRPAILSALIYSFVRAIT
                     TVSAIVFLVTPDTRVATAYILNRVEDGEYGVAIAYGSILIVVMLAIIFLFDWLIGEAR
                     ISRSKAKNQA"
     gene            complement(30099..31130)
                     /locus_tag="ACHJWA_002042"
     CDS             complement(30099..31130)
                     /locus_tag="ACHJWA_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO4308640.1"
                     /translation="MKMTFTSTLIASAVALATLTGAAQAKGRLVVYCSATNEMCEAET
                     KAFGEKYDVKTSFIRNGSGSTLAKVDAEKKNPQADVWYGGTLDPQSQAGEMGLLQAYK
                     SPNLEQIMEKFRDPAKVKGNLSSAVYVGILGFGVNTDRLKEKNLPVPKCWKDLTKPEY
                     KGEIQIADPQSSGTAYTALATFAQLWGEDQAFDYLKQLNANVSQYTKSGIAPARNAAR
                     GETAIGIGFLHDYSLEKEQGAPLELISPCEGTGYEIGGVSILKGARNLDNAKLFVDWV
                     LSKEAQELAWKKGKSYQILTNTTAETSPNSLKLDDLKLINYDMDKYGSTDVRKALINK
                     WVSEVKMGK"
     gene            complement(31127..32443)
                     /gene="uhpC"
                     /locus_tag="ACHJWA_002043"
     CDS             complement(31127..32443)
                     /gene="uhpC"
                     /locus_tag="ACHJWA_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFO4308641.1"
                     /translation="METTMHARSAREINQCYRTLRPRLLMCMIIGYAAFYLTRKSVNY
                     VLPALQTDLGLDKGDIGLLGSLFYLSYGLSKFTAGLWHDSHGQRGFMGVGLFATGLLN
                     VVFAFGESLTLLLVVWTLNGFFQGWGWPPCARLLTHWYSRNERGFWWGCWNMSINIGG
                     AIIPLISAFAAHWWGWQAAMLTPGIISMALGIWLTLQLKGTPQEEGLPTVGHWRHDPL
                     ELRQEQQSPPMGLWQMLRTTMLQNPLIWLLGVSYVLVYVIRIALNDWGNIWLTESHGV
                     NLLSANATVMLFEVGGLLGALFAGWGSDLLFSGQRAPMILLFTLGLMVSVAALWLAPV
                     HHYALLAVCFFTVGFFVFGPQMLIGLAAVECGHKAAAGSITGFLGLFAYLGAALAGWP
                     LSLVIERYGWPGMFSLLSVAAVLMGLLLMPLLMAGITTTHARRIKQ"
     gene            complement(32516..34057)
                     /locus_tag="ACHJWA_002044"
     CDS             complement(32516..34057)
                     /locus_tag="ACHJWA_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFO4308642.1"
                     /translation="MSRNLRHWVILLFIVLAWGTGWLMLWTLSFYLTNNGQQAVLFLP
                     QGVYLALVILLSRRYWPALVLPPLLMMFWLHSEQLLNGYLMLATPVISLFPALLAQNF
                     WHRFPLYWQRLTLLLATVTAASLLNTALLSPFMSGPIMLPGLTSFTGGVLLTPFVYLI
                     FEFLRQQHRYQLLGLDTHNPPLRTSLIIWCSLFFIIGIGTQMVLSPEIERLLLIVVFL
                     PNVVMAWKFGWQGGVLSGLLGSMMITIARQIGVGFSNLVELEIFLATQALLGIGLGIA
                     ISRQQHLALNLHHYRQRLEAELAARRALAEKLIHTEEDTRKNLARELHDEIGQNITAI
                     QIQSQLVKRARDPAQIQSAASQINELARRIHLSTRQLLRQLRPPALDELTFREALLHL
                     INEFAFSERGIHCQFAYQLNSTPENETVRFTLYRLLQELLNNICKHAEASEVTIILRQ
                     QGEVLHLEVSDNGVGIASGKMAGFGIQGMRERVSALGGDLTLEKQHGTRVIVNLPTFL
                     QQIAG"
     gene            complement(34057..34686)
                     /locus_tag="ACHJWA_002045"
     CDS             complement(34057..34686)
                     /locus_tag="ACHJWA_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFO4308643.1"
                     /translation="MIRVVLVDDHVVVRSGFAQLLSLEDDLEVIGQYSSAAQAWSALI
                     RDDVNVAVIDIAMPDENGLSLLKRLRAQKPQFRAIILSIYDAPTFVQSALDAGASGYL
                     TKRCGPEELVQAVRSVGLGGHYLCADAIRALRGGGQPAQALEILTPREREVFELLVKG
                     DSVKEIAFKLELSHKTVHVHRANVLGKLNCHSTIELVHFALDHHLLAGH"
     gene            34989..35951
                     /gene="tauA"
                     /locus_tag="ACHJWA_002046"
     CDS             34989..35951
                     /gene="tauA"
                     /locus_tag="ACHJWA_002046"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO4308644.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIATPDVWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            35964..36731
                     /gene="tauB"
                     /locus_tag="ACHJWA_002047"
     CDS             35964..36731
                     /gene="tauB"
                     /locus_tag="ACHJWA_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFO4308645.1"
                     /translation="MLQISHLYADYGGKPVLEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSPGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            36728..37555
                     /gene="tauC"
                     /locus_tag="ACHJWA_002048"
     CDS             36728..37555
                     /gene="tauC"
                     /locus_tag="ACHJWA_002048"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFO4308646.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVLIGIPVG
                     IAMGLSPTVRGILDPIIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            37552..38403
                     /gene="tauD"
                     /locus_tag="ACHJWA_002049"
     CDS             37552..38403
                     /gene="tauD"
                     /locus_tag="ACHJWA_002049"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFO4308647.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(38510..39484)
                     /gene="hemB"
                     /locus_tag="ACHJWA_002050"
     CDS             complement(38510..39484)
                     /gene="hemB"
                     /locus_tag="ACHJWA_002050"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFO4308648.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT         9126 a        10474 c        10887 g         9177 t
ORIGIN      
        1 ctgatgcgac gctgatgcgt cttatcatgc ctacaggcct gaaccgtagg tcggataagg
       61 cgctcgcgcc gcattcgaca ccatgcgccg atgcctgatg cgacgcaggc gcgtcttatc
      121 atgcctacag gcctgaaccg taggtcggat aaggcgctcg cgccgcatcc gacaccatgc
      181 gcagatgcct gatgcgacgc tgatgcgtct tatcatgcct acaggcctga accgtaggtc
      241 ggataaggcg ctcgcaccgc atccgacacc gtgctcagtg cctgatgcga cgctgatgcg
      301 tcttatcatg cctacaggcc tgaaccgtag gtcggataag gcgctcgcgc cgcatccgac
      361 accgtgcgca gatgcctgat acgacgcagg cgcgtcttat catgcctaca ggcctgaacc
      421 gtaggtcgga taaggcgctc gcgccgcatc cgacaccgtg cgtagttgcc tgatgcgacg
      481 ctgacgcgtc ttatcatgcc tacccactgt ttttacaccg ataattttcc ccccaccttt
      541 ttgcactcat tcatataaaa aatatatttc cccacgaaaa cgattgcttt ttatcttcag
      601 atgaatagaa tgcggcggat tttttgggtt tcaaacagca aaaaggggga atttcgtgtc
      661 gcaagataac aactttagcc aggggccggt cccgcagtcg gcgcggaaag gggtattggc
      721 attgacgttc gtcatgctgg gattaacctt cttttccgcc agtatgtgga ccggcggcac
      781 tctcggaacc ggtcttagct atcatgattt cttcctcgca gttctcatcg gtaatcttct
      841 cctcggtatt tacacttcat ttctcggtta cattggcgca aaaaccggcc tgaccactca
      901 tcttcttgct cgcttctcgt ttggtgttaa aggctcatgg ctgccttcac tgctgctggg
      961 cggcacccag gttggctggt ttggcgtcgg tgtggcgatg tttgctattc cggtgggcaa
     1021 ggcaaccggg ctggatatta atttgctgat tgccgtttcc ggtttactga tgaccgtcac
     1081 cgtcttcttt ggcatttcgg cgctgacggt tctttcgttg attgcggttc cggctatcgc
     1141 ctgtcttggc ggttattccg tgtggctggc cgttaacggc atgggcggcc tggacgcctt
     1201 aaaagcggtc gttcccgcac aaccgttaga tttcaatgtc gcgctggcgc tggttgtggg
     1261 gtcatttatc agtgcgggca cgctcaccgc tgactttgtc cgctttggtc gcaatgccaa
     1321 actggcggtg ctggtggcga tggtggcctt tttcctcggc aactcgttga tgtttatttt
     1381 cggtgcagcg ggcgctgcgg cactggggat ggcggatatc tctgatgtga tgattgctca
     1441 gggcctgctg ttgcctgcga ttgtggtgct ggggctaaat atctggacca ccaacgataa
     1501 cgcgctctat gcgtcgggtt taggtttcgc caacattacc gggatgtcga gcaaaaccct
     1561 ttcggtaatc aacggtatta tcggtacggt ctgtgcatta tggctgtata acaattttgt
     1621 cggctggttg accttccttt cggcagctat tcctccagtg ggtggcgtga tcattgccga
     1681 ctatctgatg aaccgtcgcc gctatgagca ctttgcgacc acgcgtatga tgagtgtcaa
     1741 ttgggtggcg attctggcgg tcgccctggg gattgctgca ggccactggt taccgggaat
     1801 tgttccggtc aacgcggtat taggtggcgc gctgagctat ctgatcctta acccgatttt
     1861 gaatcgtaaa acgacagcag caatgacgca tgtggaggct aacagtgtcg aataacgctt
     1921 tacaaacaat tattaacgcc cggttgccag gcaaagaggg gctgtggcag attcatctgc
     1981 aggacggaaa aatcagcgcc attgatgcgc aatccggcgt gatgcccata actgaaaaca
     2041 gcctggatgc cgaacaaggt ttagttatac cgccgtttgt ggagccacat attcacctgg
     2101 acaccacgca aaccgccgga caaccgaact ggaatcagtc cggcacgctg tttgaaggca
     2161 ttgaacgctg ggccgagcgc aaagcgttat taacccatga cgatgtgaaa caacgcgcat
     2221 ggcaaacgct gaaatggcag attgccaacg gcattcagca tgtgcgtacc catgtcgatg
     2281 tttcggatgc aacgctaact gcgctgaaag caatgctgga agtgaagctg gaagtcgcgc
     2341 cgtggattga tctgcaaatc gtcgccttcc ctcaggaagg gattttgtcg tatcccaacg
     2401 gtgaagcgtt gctggaagag gcgttacgct taggggcaga tgtagtgggg gcgattccgc
     2461 attttgaatt tacccgtgaa tacggcgtgg agtcgctgca taaaaccttc gccctggcgc
     2521 aaaaatacga ccgtctcatc gacgttcact gtgatgagat cgatgacgag cagtcgcgct
     2581 ttgtcgaaac cgttgctgcc ctggcgcacc gtgaaggcat gggcgcgcga gtcaccgcca
     2641 gccacaccac ggcaatgcac tcttataacg gggcgtatac ctcacgtctg ttccgcttgc
     2701 tgaaaatgtc cggtattaac tttgtcgcca acccgctggt caatattcat ctgcaaggac
     2761 gattcgatac gtatccaaaa cgtcgcggca tcacgcgcgt taaagagatg ctggagtccg
     2821 gcattaacgt ctgctttggt cacgatgatg tcttcgatcc gtggtatccg ctgggaacgg
     2881 cgaatatgct gcaagtgctg catatggggc tgcatgtttg ccagctgatg ggctatgggc
     2941 agattaacga tggcctgaat ttaatcaccc accacagcgc caggacgttg aatttgcagg
     3001 attacagcat tgccgccgga aacagcgcca acctgattat cctgccggct gaaaatggat
     3061 ttgatgcgct gcgccgtcag gttccggtac gttattcggt acgtggcggc aaggtgattg
     3121 ccagcacaca accggcacaa accaccgtat atctggagca gccggaagcc atcgattaca
     3181 aacggtgatc ggctgggctg tgtgttgaca aagcgagcgt agtttatgcc ggatgcggct
     3241 tgaacgcctt atcaggccta cgtagagcac tgaactcgta ggcctgataa gcgtaacgca
     3301 tcaggcaatt gtgtcagtgg ttaccgcgat tcattctcgc caacatccgc acattcctcc
     3361 aacgccatgt gcaaaaatgc cttcgcggcg gctgtctgcc agctattttt ccgccgcaac
     3421 aaaaccgccg ttctctccag tagtggcggg gcaagaggaa tcgctttaag cccgtcatgt
     3481 tgtgtggcaa tcgctgctgg taacaatgtg gaaagggaag tgcggcgaat cagctccaga
     3541 accgcgctaa ttgagttcgc ctcaatgacc acctgtggat gcagccccgc tttctcgcag
     3601 tagtggtcaa tctgctctct ggtggcaaat tccgcgctga gcaggaccag tttttcatca
     3661 tgcaagcgac tcaacgccac ctgttcacag gcagccagcg gatgatgttg cgccacgact
     3721 aacgctaaac tttctgtcag taaaggaatt gcctccagct ccggcaaatg cacaggcgcg
     3781 aaggcaatcc caacgtccag ctcgtcgcga caaagcagat cttcgatttt ctcctgcgac
     3841 atttcctgta gctggagcgt gatgccgggg tagcgcgcat agaaatccgc cattaagggg
     3901 ccgataaagt agctcgtaaa ggtgggggtg acggcgatac gcagcgatcc tctcgtcaga
     3961 tcggcaacat catgaatcgc ccgtttaccc gctcccagtt cctgtaacgc ccggctggcg
     4021 tactgtcgcc agacttctcc tgcatcagtg agatgaatcg ttcgcccgct acggtcaaac
     4081 agcggcacgc ctaaactctc ctctaactgg cgaatctgct gggaaagcgc aggctgagag
     4141 acgtgcaacg cactggcggc acgggtgaag ctgccatgtt cagccacggc aagaaaataa
     4201 ttgatatgtc gagagagcat tcgccaacct ataagtaaat tcaatagaac tcatcataaa
     4261 tgagactttt accttatgac aatcggcgag tagtctgcct ctcattccag agacagatag
     4321 aggttaacgg tgaaagagat tattgatgga ttccttaaat tccagcgcga ggcatttccg
     4381 aagcgggaag ccttatttaa acagttggcg acacagcaaa gcccgcgcac actttttatc
     4441 tcctgctccg acagccgtct ggtccctgag ctggtgacgc aacgtgagcc tggcgatctt
     4501 ttcgttattc gcaatgcggg caatatcgtc ccttcctacg ggccggaacc cggtggcgtt
     4561 tctgcttcgg tggagtatgc cgtcgctgcg cttcgggtat ctgacattgt gatttgcggt
     4621 cattccaact gtggcgcgat gaccgccatt gccagctgtc agtgcatgga ccatatgcct
     4681 gccgtctccc actggctgcg ttatgccgat tcagcccgcg tcgttaatga ggcgcgcccg
     4741 cattccgatt taccgtcaaa agctgcggcg atggtacgtg aaaacgtcat tgctcagttg
     4801 gctaatttgc aaactcatcc atcggtgcgc ctggcgctcg aagaggggcg gatcgccctg
     4861 cacggctggg tctacgacat tgaaagcggc agcatcgcag cgtttgacgg cgcaacccgc
     4921 cagtttgtgc cactggcagc taatcctcgc gtttgtgcca taccgctacg ccaaccgacc
     4981 gcagcgtaac cttattttta aaccatcagg agatgcacca tgattcagtc gcaaattaac
     5041 cgcaatattc gtcttgatct tgccgatgcc attttgctca gcaaagctaa aaaagctctc
     5101 tcatttgccg agattgccga cggcaccggg ctggcagaag cctttgtgac cgcggctttg
     5161 ctgggtcagc aggcgcttcc tgccgacgcc gcccgccagg tcggggcgaa gctggatctc
     5221 gacgaagacg ccattctact gttacagatg atcccactgc gtggctgcat tgatgaccgt
     5281 attccaaccg acccaacgat gtatcgtttc tatgaaatgt tgcaggtgta cggcacaacc
     5341 ctgaaagcgt tggttcatga gaaatttggc gatggcatta tcagcgcgat taacttcaaa
     5401 ctcgacgtta agaaagtggc ggacccggaa ggtggcgaac gtgcggtcat caccttagat
     5461 ggtaaatatc tgccgaccaa accgttctga cagccatgcg taaccatcaa aagacgttca
     5521 cgatgctgct ggttctggtg ctgattggtc ttaatatgcg accactgctc acctccgtcg
     5581 ggccactgct accgcaattg cgccaggcaa gcggaatgag ctttagcgtg gctgccctgt
     5641 tgaccgctct gccggtggtt accatgggcg ggctggcgct ggccggaagc tggcttcatc
     5701 agcatgtcag cgaacgtcgc agcgtcgcca tcagtttgtt actgattgcc gtcggtgcat
     5761 tgatgcgtga gctttaccct caaagtgcgc tgctgcttag cagcgcactg ctcggtgggg
     5821 tggggatcgg catcattcag gcggtgatgc cttcggtgat taaacggcgg tttcagcagc
     5881 gcacgccact ggtgatgggg ctgtggtccg cggctctgat gggcggcggt gggcttggtg
     5941 ccgccctgac accctggtta gttcaacata gcgaaacctg gtatcaaaca ctcgcctggt
     6001 gggcgctgcc tgccgttgtt gcgctctttg cctggtggtg gcaaagcacc cgcgaggtcg
     6061 cctcttccca caagacaaca accattccgg ttcgcgtggt tttcactcct cgcgcgtgga
     6121 cgctgggtgt ttacttcggt ctgattaacg gtggttacgc cagcctgatt gcctggttac
     6181 ccgccttcta tattgagatt ggtgccagcg cgcagtacag cggttccttg ctggcattga
     6241 tgacgcttgg gcaagccgca ggagctttgc tgatgcctgc tatggctcgc catcaggatc
     6301 ggcgcaaact gttaatgctg gcgctggcgt tacaactggt gggtttctgc ggctttatct
     6361 ggctgccgct gcaattgccg gtattgtggg cgatggtgtg tggtttaggt ctgggcggcg
     6421 catttccgct ctgtttgctg ctggcgctcg atcactctgt gcaaccggct attgctggca
     6481 agctggtggc gtttatgcag ggaatcggtt ttatcatcgc cgggcttgcc ccgtggtttt
     6541 ctggcgtgct gcgtagtatc agcggcaatt acttgatgga ctgggcgttt catgcgctgt
     6601 gcgtcgtagg gctgatgatt ataaccctgc gttttgcacc agcacgtttt ccgcagctgt
     6661 gggtcaaaga ggcatgatgc gacgcttgtt tctgcgcttt gttcacgccg gatgcggcct
     6721 acatagatca ctgaatttgt aggcctgata agcgaagcgt atcaggcaat ttttataatt
     6781 taaactgacg attcaacttt gtaatctctg aaataatatt gcttatcccg gtcgtttatt
     6841 tcgcgaataa cccgacaagg aacgccagcc gccacgacgt ttggtggaat gtcttttgtg
     6901 acgatactac ccgcgccaat aacagaatta tccccaatgg tgacgcctgg attaataacc
     6961 acatgacttc cgatccagac gttattgcca atcgttatcg gaaaagagta catctcaccg
     7021 ttttttctca attcatggtg tacagggtgt cccgtaacgg aaagagtaac gttgggtgca
     7081 atcaatacgt tatcaccgat tgttaccgtg tagtcatcga caatggttaa attgaaattt
     7141 gcataaaaat tgcggcctat atggatgttg gaaccgtaag agaaatagac ggggggttct
     7201 acccaggcgt tttcccctac cgtggcaaac atttctttaa tcaggctttc tcttttatca
     7261 acttctgatg gatgcgagtg attaaactca tacattaacg tttttctacg aagtcttttt
     7321 tcaggtaagc cttcgcacat atcggtaaat agcttgcctg cttttattct ttctgtcatc
     7381 gacatgttca ttgcgatcat tccgttatga tatgttggtc ggataaggcg ctcgcgccgc
     7441 atccgacatt gattgcttaa gcgacttcat tcacctgacg acgcagtaga gaaagcgggc
     7501 cggggccgct aagcgtgaac acggaaatta aggtgaagcc cagcgccacc agacccagca
     7561 ccagataagc gccctggaaa ccgatgcttt catacatatt acccgctagt acagacataa
     7621 aaatcatcgc cagttgctta aagaagcaga aacagaccag ataaatcgtc gctgaaaaac
     7681 gcacttcaaa ctggctggta atatatttaa agcagcccac cagcaggaac ggtacttcaa
     7741 acatatgcag cgttttcaga ataaccactt ccagcgctga ggtggcgaac gatgagccaa
     7801 taatacgtac agacataata gtgccagcca gcagcagggc atttttccca ccgatgcgat
     7861 taatgatcag tggcgcaaag aacataattg aggcgttaag taattcgccc attgtcgtta
     7921 cgtagccaaa tacccgcgta ccctgttcac cggtggcaaa gaaagaagta aagaaattag
     7981 caaactgttg gtcaaaaaca tcgtaggtgc aggaaacgcc aataacatac agtaacaaaa
     8041 accacagttt tggctgtctg aacagttcca gcgccagttt aaggctaaat gccgaatggt
     8101 tggcacctac cgcattggca accgtggcgg aagagggcgc atccgttttg gcgaaaaaga
     8161 gtaaaatggc gaggatgagt gcacagccag aacccagcca gaaaacgaac tgattattga
     8221 tggtgaacat gatgccgaca atcgaggcac acagcgccca gccaacacag ccaaacatcc
     8281 gcgcgcgacc aaattcgaaa ttactgcgac ggctgacttt ctcgataaat gcctctactg
     8341 cgggcgcacc ggcgttaaaa caaaagccaa gataaatacc accaacaatc gatcctacta
     8401 aaatgttgta ttgtaacagt ggcccgaaga taaaaataaa gaacggcgca aacatcacta
     8461 acatgccggt aataatccac agcaggtatt tgcgcagccc gagtttgtca gaaagcagac
     8521 caaacagcgg ttggaataat agcgagaaca gagaaatagc agcaaaaata atacccgtat
     8581 cacttttgct gatatggttg atgtcatgta gccaaatcgg gaaaaacggg aagtaggctc
     8641 ccatgataaa aaagtaaaag aaaaagaata aaccgaacat ccaaaagttt gtgtttttta
     8701 aatagtacat aatggatttc cttacgcgaa atacgggcag acatggcctg cccggttatt
     8761 attatttttg acaccagacc aactggtaat ggtagcgacc ggcgctcagc tggaattccg
     8821 ccgatactga cgggctccag gagtcgtcgc caccaatccc catatggaaa ccgtcgatat
     8881 tcagccatgt tccttcttcc gcgtgcagca gatggcgatg gctggtttcc atcagttgtt
     8941 gttggctgta gcggctgatg ttgaactgga agtcgccgcg ccactggtgt gggccataat
     9001 tcaattcgcg cgtcccgcag cgcagaccgt tttcgctcgg gaagacgtac ggggtataca
     9061 tgtctgacaa tggcagatcc cagcggtcaa aacaggcggc agtaaggcgg tcgggatagt
     9121 tttcttgcgg ccctaatccg agccagttta cccgctctgc tacctgcgcc agctggcagg
     9181 tcaggccaat ccgtgccgga tgcggtgtat cgctcgctac ttcaacatca acggtaatcg
     9241 ccatttgacc actaccatca atccggtagg ttttccggct aataaataag gtttttccct
     9301 gatgctgcca tgcgtggaca gtggtaatca gcaccgcgtc ggcaagtgta tctgccgtgc
     9361 actgcaacaa cgccgcttct gcctggtaat ggcccgcagc cttccagcgt tcgacccagg
     9421 cgttagggtc aatgcgggtc gcttcactta cgccaatgtc gttatccagc ggtgcgcggg
     9481 tgaactgatc gcgcagcggc gtcagcagtt gttttttatc gccaatccac atctgtgaaa
     9541 gaaagcctga ctggcggtta aattgccaac gcttattatc cagctcgatg caaaaatccg
     9601 tttcgctggt ggtcagttgc gggatggcgt ggggcgcgga ggggagtgtc acgctgaggt
     9661 tttccgccag acgccactgc tgccaggcgc tgatgtgtcc ggcttctgac catgcggtcg
     9721 cgttcggttg cactacgtgt accgttagcc agagttgccc ggtgctctcc agtcgcggta
     9781 gttcaggcaa ttcaattacc tgtttacctt gcggagcgac atccagaggc acttcaccgc
     9841 ttgccagcgg cttaccatcc agcgccaccg tccagtgcag gagctcgtta tcgctatgac
     9901 ggaacaggta ttcgctggtc acttcgatgg ttcgcccgga taaacggaac tggaaaaact
     9961 gctgctggtg ttttgcttcc gtcagcgccg gatgcggcgt gcggtcggca aagaccagac
    10021 cgttcatgca gaactggcga tcattcggcg tatcgccaaa atcaccgccg taagccgacc
    10081 acggattgcc attttcatca tatttaatca gcgactgatc caccaagtcc cagacgaagc
    10141 cgccctgtaa acgggggtac tgacgaaacg cctgccagta tttagcgaag ccgccaagac
    10201 tgttacccat cgcgtgggcg tattcgcaaa ggatcagtgg gcgcatttct ccaggcagcg
    10261 aaagccattt tttgatggac catttcggca cagccgggaa gggctggtct tcatccacgc
    10321 gcgcgtacat cgggcaaata atatcggttg cggaggtgtc ggctccgccg ccttcatact
    10381 gcaccgggcg ggatggatcg acagatttaa tccagcgata gagtgcgtcg tgattagcgc
    10441 cgtggcctga ctcattcccc agcgaccaga tgatcacact cgggtgatta cgatcgcgct
    10501 gcaccattcg tgttactcgt tcgctcatgg ccggtaacca gcgcggatca tcggtcagac
    10561 gattcattgg caccatgccg tgggtttcaa tattggcttc atccaccaca tacaggccgt
    10621 agcggtcgca cagggtgtac cacagcggat ggttcggata atgcgaacag cgcacggcgt
    10681 taaagttgtt ctgcttcatt agcaggatat cctgcaccat cgtctgctca tccatgacct
    10741 gaccatgcag aggatgatgc tcgtgacggt taacgccgcg aatcagcaac ggcttgccgt
    10801 tcagcagcag cagaccattt tcaatccgca cctcgcggaa accgacatcg caggcttctg
    10861 cttcaatcag cgtgccgtcg gcggtgtgca gttcaaccac cgcacgatag atattcggga
    10921 tttcggcgct ccacagtttc gggttttcga cgttcagacc tagtgtgacg cgatcggcat
    10981 aaccgccacg ctcatcgata atttcaccgc cgaaaggcgc ggtgccgctg gcgacctgcg
    11041 tttcaccctg ccacaaagaa accgtcaccc gcagctcatc gcgcagctcg ccgtacatct
    11101 gaacttctgc ctccagtacc gcgcggctaa aatcatcatt aaagagagtg gcaacatgga
    11161 aatcgctgat ttgtgtggtc ggtttgtgca gcaacgagac gtcacggaaa atgccgctca
    11221 tccgccacat atcctgatct tccagataac tgccgtcact ccagcgcagc accatcaccg
    11281 cgaggcggtt ttctccggcg cgtaaaaatg cgctcaggtc aaattcagac agcaaacgac
    11341 tgtcctggcc gtaaccgacc cagcgcccgt tgcaccacag atgaaacgcc gagttaacgc
    11401 catcaaaaat aattcgcgtc tggccttcct gtagccagct ttcatcaaca ttaaatgtga
    11461 gcgagtaaca acccgtcgga ttctccgtgg gaacaaatgg cggattgacc gtaatgggat
    11521 atgtcacgtt ggtgtagatg ggcgcgtcgt aaccgtgcat ctgccagttt gaggggacga
    11581 cgacagtatc ggcgtcagga agatcgcact ccagccagct ttccggaacc gcttctggtg
    11641 ccggaaacca gacaaattgc cactcaccat tcaggctgcg caactgctgg gaagggcgat
    11701 tggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
    11761 taagttgggt aacgccaggg ttttcccagt cacgacgttg taatacgacg gccagtgaat
    11821 ctgtaatcat agtcatagct gtatcctgtg tgaaattgtt atccgctcac aatttcacac
    11881 aacatacgag ccggacgcat agagtgtaaa gcgtggggtg cctaatgagt gagctgactc
    11941 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacttgtc gtgccagctg
    12001 cattaatgaa tcggccaacg cgcggggaga agcggtttgc gtcttgggcg ccagggtggt
    12061 ttttcttttc accagtgaga cgggcaacag ctgattgccc ttcaccgcct ggccctgaga
    12121 gagttgcagc aagcggtcca cgctggtttg ccccagcagg cgaaaatcct gtttgatggt
    12181 ggttaacggc gggatataac acgagctgtc ttcggtatcg tcgtatccca ctaccgagat
    12241 atccgcacca acgcgcaacc cggactcggt aatggcgcgc attgcgccca gcgccatctg
    12301 atcgttggca accagcatcg cagtgggaac gatgccctca tttagcattt gcatggtttg
    12361 ttgaaaaccg gacatggcac tccagtcgcc ctcccgttcc gctatcggct gaatttgatt
    12421 gcgagtgaga tatttatgcc agcccgccag acgcagacgc gccgagacag aacttaatgg
    12481 gcccgctaac agcgcgattt gctggtgacc caatgcgacc agatgctcca cgcccagtcg
    12541 cgtaccgtct tcatgggaga aaataatact gttgatggga gtctggtcag agacatcaag
    12601 aaataacgcc ggaacattag cgcaggcagc ttccacagca atggcatcct ggtcatccag
    12661 cggatagtta atgatcagcc cactgacgcg ttgcgcgaga agattgtgca ctgccgcttt
    12721 acaggcttcg acgccgcttc gttctaccat cgacaccacc acgctggcac ccagttgatc
    12781 ggcgcgagat ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca gactggaggt
    12841 ggcaacgcca atcagcaacg actgtttccc cgccagttgt tgtgccacgc ggttgggaat
    12901 gtaattcagc tccgccatcg ccgcttccac tttttcccgc gttttcgcag aaacgtggct
    12961 ggcctgattc accacgcggg aaacggtctg ataagagaca ccggcatact ctgcgacatc
    13021 gtatagcgtt actggtttca cattcaccac cctgaattga ctctcttccg ggcgctatca
    13081 tgccataccg cgaaaggttt tgcgccattc ggcggggctg acgccgaagt gagatttaaa
    13141 atgctgacgc caggtcacag gtgagagaaa acccacctca gccgctatcc gctcaacggg
    13201 caactgactg gatcccaaca gttcctggct acggcgtaag cgttcagtaa tgagccattc
    13261 ggcgatactc gaaccggtcg ctttcataaa atggcgagtt aatgtgcggc gactcatcat
    13321 tactcgctgc gccagcgagt cgagatcatg ctgttgcgca atgttttgcc gcaggtaatc
    13381 cagcaggagg tttatgcgtt catcatgggt atttttcggc accggttgct caataaactg
    13441 cgcctgaccg ccggtgcgat atggcggtac gaccatccgt cgggcaatat ggttagcata
    13501 gtcgctgcca aaatgttgcc gaacaatata caaacagcaa tccagcgccg cggcagtacc
    13561 ggcagaggta ataatgccgt catcgtctac gtacaacgcg ttaatatcca gatgtgtttg
    13621 tggaaaacgt gcctgaaatt cacgctcgaa ctcccagtgc gtggcggcac gcttattttt
    13681 cagtaaacct gcatagccga gcacaaacgt gcccaggcaa agcccgacaa tctgtgcgcc
    13741 gttatcccgt gcggtcgtta aggcttccag cagtttttgt ggcggttttt gtgtaatggt
    13801 tccccagtaa ggaattatca caatatctgc ctggatgact gcggtatagt catgggtggc
    13861 ggttacggaa aacccatcgg cagagtccac gtttcccggc ttttcagcgc aaataatcac
    13921 gtgaaagcgt tttttctccg ccatcttttc actaaacacg ataaacggta cggaaaggtg
    13981 aaaaggacta aaaccggcaa cggcgataat ggcaacagtt agcgggcgca taacactctt
    14041 tatctccgaa ttagaatgtc aacgtttcac catcttctgg tacgttcaca aattcgataa
    14101 tttgattatc tctggcgtat tccttgagcg catcacgcgt cagcaaacag tggtttacgg
    14161 cctccatatg tgtcgcgata atttgcgctt ctgggagggt aaaatgtgtt ttgagaatat
    14221 cttctgctcc cataattatc gcgccatgcc ccagaacatg cgcaaatccg gcattcaaca
    14281 caaccacttc tggttggaaa gttagcattg tagcctcgac gtcgtcacgc cagagggtat
    14341 cgccagccag atagagcgtt ttctctgtcg ggtgctggaa aacaacgccg caagctgcgc
    14401 ctaaacgctt cgccatcgcc gggtcggcat aggtgcgatc ggagccgtgc tgccctgtcg
    14461 ttttatgcag agtaatatcg ccaaatttag tctcgtcggt aagtacggta agatgcgtaa
    14521 atccttgttt attcagtgat tcagcatctt gctcattttg cacataaatc agtttatctt
    14581 tagggacgac gcgggaggct gtttcgtccc agtgatcttc atgcaaatgg gtcacgatta
    14641 gggcgtcaac gttgagtaac gttgttaaat cgcacggtaa ttctaccgtt ggattgcgga
    14701 tttctgcgcg tgccgttccg gcaaaacccg gataacttcc tttaggcgcg agtaacgggt
    14761 cgataagaaa tgttttgcca gcgtattgga taatttgggt ggcattacga atttgcgtaa
    14821 ttttcatgat ttgctccaca tcacagtatt gatgtcatta gcgtagcgtg cagcccgtac
    14881 tgtgcatgtg ggcaaatggg cgaataacga tgagaatggg ccaaattaca cattggcgta
    14941 actagcgtaa atgcacgccg cttcgccgtc cggccaccag aatagcctgc gattcaaccc
    15001 cttcttcgat ctgttttgct acccgttgta gtgccggaag atgcttttcc gctgcctgtt
    15061 cgatggtcat tgcgctcgcc atatacacca gattcagaca gccaatcacc cgttgttcac
    15121 tgcgcagcgg tacggcgata gaggcgatct tctcctcctg atcccagccg cggtagttct
    15181 gtccgtaacc ctctttgcgc gcgcgcgcca gaatggcttc cagctttaac ggttctcgtg
    15241 ccagttgata gtcatcaccg gggcgggagg ctaacatctc gattaattcc ttgcgttctt
    15301 gttccgggca aaaggccagc caggtcaggc ccgaggcggt tttcagaagc ggtaaacgcc
    15361 gcccgaccat tgcccggtga aaggataagc ggctgaaacg gtgagtggtt tcgcgtacca
    15421 ccattgcatc aacatccagc gtggacacat ctgtcggcca taccacttcg cgcaacagat
    15481 cgcccagcag tggggccgcc agtgcagaaa tccactgttc gtcacgaaat ccttcgctta
    15541 attgccgcac tttgatggtc agacgaaaac tatcatcgga gggactacgc cggacatatc
    15601 cctcttcctg cagcgtctcc agcagtcgcc gcacagtggt gcgatgcagg ccgctgagtt
    15661 ccgccagcag cccgacgctg gcaccgccgt caagtttatt taacatattt aataacatta
    15721 gaccgcgggt taagccgcgc acggttttgt attccgtctg ctcattgttc tgcatattaa
    15781 ttgacatttc tatagttaaa acaacgttgt gcacctggtg cacattcggg catgttttga
    15841 ttgtagccga aaacaccctt tctatactga gcgcacaata aaaaatcatt tacatatttt
    15901 taacaaaata agttgcgctg tactgtgcgc gcaacggcat tttgtccgag tcgtgaggta
    15961 ctgaaatggc aatacaacac cctgacatcc agcctgctgt taaccatagc gttcaggtgg
    16021 cgatcgctgg tgccggtccg gtcgggctga tgatggcgaa ctatctcggc cagatgggca
    16081 ttgacgtgct ggtggtggag aaactcgata agttgatcga ctacccgcgt gcgattggta
    16141 ttgatgacga ggcgctgcgc accatgcagt cggtcggcct ggtcgataat gttctgccgc
    16201 atactacgcc gtggcacgcg atgcgttttc tcaccccgaa gggccgctgt tttgctgata
    16261 ttcagccaat gaccgatgaa tttggctggc cgcgccgtaa cgcctttatt cagccgcagg
    16321 tcgatgcggt gatgctggaa ggggtgtcgc gttttccgaa tgtgcgctgc ttgttttccc
    16381 gcgagctgga ggccttcagt cagcaagatg acgaagtgac cttgcacctg aaaacggcag
    16441 aagggctgcg ggaaatagtc aaagcccagt ggctggtggc ctgtgatggt ggggcaagtt
    16501 ttgtccgtcg caccctgaat gtgccgtttg aaggtaaaac tgcgccaaat cagtggattg
    16561 tggtagatat cgccaacgat ccgttaagta cgccgcatat ctatttgtgt tgcgatccgg
    16621 tgcgcccgta tgtttctgcc gcgctacctc atgcggtacg tcgctttgaa tttatggtga
    16681 tgccgggaga aaccgaagaa cagctgcgtg agccgcaaaa tatgcgcaag ctgttaagca
    16741 aagtgctgcc taatccggac aatgttgaat tgattcgcca gcgtgtctac acccacaacg
    16801 cgcgactggc gcaacgtttc cgtattgatc gcgtactgct ggcgggcgat gccgcgcaca
    16861 tcatgccggt atggcagggg cagggctata acagtggtat gcgcgacgcc tttaacctcg
    16921 catggaaact ggcgttggtt atccagggga aagcccgcga tgcgctgctc gatacctatc
    16981 aacaagaacg tcgcgatcac gccaaagcga tgattgacct gtccgtgacg gcgggcaacg
    17041 tgctggctcc gccgaaacgc tggcagggta cgttacgtga cggcgtttcc tggctgctga
    17101 attatctgcc gccagtaaaa cgctacttcc tcgaaatgcg cttcaagccg atgccgcaat
    17161 attacggcgg tgcgctggtg cgtgagggcg aagcgaagca ctctccggtc ggcaagatgt
    17221 ttattcagcc gaaagtcacg ctggaaaacg gcgacgtgac gctgctcgat aacgcgatcg
    17281 gcgcgaactt cgcggtaatt ggctggggat gcaatccact gtgggggatg agcgacgagc
    17341 aaatccagca gtggcgcgcg ttgagcacac gcttcattca ggtggtgccg gaagtgcaaa
    17401 ttcataccgc acaggataac cacgacggcg tactacgcgt gggggatacg caaggtcgcc
    17461 tgcgtagctg gttcgcgcaa cacaatgctt cgctggtggt gatgcgcccg gatcgctttg
    17521 ttgccgccac cgccattccg caaacactgg gcaagaccct gaataaactg gcgtcggtga
    17581 tgacgctgac ccgccctgat gccgacgttt ctgtcgaaaa ggtagcctga tatgcacgct
    17641 tatcttcatt gtctttccca ctcgccgctg gtggggtatg tcgacccggc acaagaggta
    17701 ctcgatgagg tcaatggcgt gattgccagc gcccgcgagc gtattgcggc tttctcccct
    17761 gaactggtgg tgctgtttgc gccagatcac tacaacggct ttttctatga cgtgatgcca
    17821 ccgttctgtt taggcgttgg agcgacggca attggtgatt tcggcagtgc ggcaggagag
    17881 ctgcccgtgc ctgtggagct ggcggaggcc tgtgcgcatg ccgtcatgaa gagcgggatc
    17941 gatcttgccg tttcttactg tatgcaggtg gaccacgggt tcgcccagcc gctggagttc
    18001 ctgctcggtg ggctggataa ggtgccagtt ctgcctgtgt tcatcaacgg tgtcgccacg
    18061 ccgctgcccg gtttccagcg tacccgcatg ttgggtgaag ccattggacg tttcaccagc
    18121 actctcaata aacgcgtgct gttcctaggt tccggtgggc tttcccatca gccgccggtg
    18181 cccgaactgg cgaaagccga tgcccatatg cgcgaccgcc tgttggggag cgggaaagat
    18241 ttacccgcca gtgagcgcga attgcgtcag caacgggtga ttagcgccgc tgagaagttt
    18301 gttgaggatc agagaactct gcatccgctc aacccgattt gggataacca gttcatgact
    18361 ttgctggagc agggacgcat acaggaactg gatgccgtca gtaacgaaga gctttccgcc
    18421 attgccggaa agtcgacaca tgaaatcaaa acctgggtcg ccgcttttgc cgctatttct
    18481 gcatttggca actggcgtag cgaagggcgt tattaccgcc caatcccgga gtggattgcc
    18541 ggatttggct cgttaagcgc cagaacagag aactgaatat gcaggagaag atgatgagtt
    18601 atcagccaca aaccgaagcc gccaccagcc gttttctgaa tgtagaagaa gcgggtaaaa
    18661 cgctgcgcat ccattttaat gactgcggac aaggcgacga aaccgttgtc ctgctgcatg
    18721 gttccggccc gggtgctact ggctgggcga acttcagccg caatatcgat ccgctggtag
    18781 aggcgggcta tcgggtgatc ctgctggatt gtccgggttg gggcaagagc gattcgatcg
    18841 ttaatagtgg ttcgcgatcg gatcttaatg cacgaatcct gaaaagcgtg gtggatcaac
    18901 tggatatcgc caaaatccac ctgctgggca actcgatggg tggccatagt tctgtggcgt
    18961 tcacccttaa ctggccggag cgcgtcggca aactggtgct gatgggcggc ggtacgggcg
    19021 gcatgagttt gtttacgccg atgccaaccg aaggtattaa gcgactgaat cagctttatc
    19081 gtcagccgac tatcgaaaac ctgaagctga tgatggatat cttcgttttt gataccagcg
    19141 atttgaccga cgccctgttt gaagcgcgcc tgaataatat gctgtcgcgc cgcgatcacc
    19201 tggaaaactt cgttaagagc ctggaagcta atccgaaaca gttcccggat tttggcccac
    19261 gtctggcgga aatcaaagcg caaaccctga ttgtctgggg gcgcaacgac cgctttgtgc
    19321 cgatggatgc gggtctgcgt ctgctgtccg gcattgccgg ttctgaactg catatcttcc
    19381 gcgactgtgg gcattgggcg cagtgggaac atgccgacgc tttcaatcaa ctggtgctga
    19441 atttcctcgc acgtgcttaa ggaacggtca tgacgaagca tactcttgag caactggcgg
    19501 cggatttacg ccgcgccgca gagcagggcg aagcgattgc gccgctgcgc gatctgattg
    19561 gtatcgataa cgctgaagcg gcctacgcca ttcagcacat aaatgtgcaa tatgacgttg
    19621 cgcaggggcg tcgcgtggtg gggcgtaaag tgggcctgac acatccgaaa gtgcagcaac
    19681 aactgggcgt tgatcaaccg gatttcggga cattatttgc cgacatgtgt tatggcgata
    19741 acgaaatcat tcctttttcc cgtgttctgc aaccccgcat tgaagcggag atcgcactgg
    19801 tgttgaaccg cgatttaccc gcaactgata tcaccttcga cgaattgtat aacgccattg
    19861 aatgggtact tccggcgctg gaggtggtgg gtagccgcat tcgcgactgg tcgattcagt
    19921 ttgtcgatac cgtggcagat aacgcttcct gcggggtgta tgtcatcggc ggtccggcgc
    19981 aacgtccggc ggggttagac ctgaaaaact gcgccatgaa gatgacgcgt aataacgaag
    20041 aggtttccag cgggcgcggc agcgaatgcc tgggacatcc gcttaatgcc gccgtctggc
    20101 tggcacgcaa aatggccagt ctgggtgaac cgctgcgcgc cggagatatc attcttaccg
    20161 gggcattagg tccgatggtg gcagtgaatg cgggcgatcg ttttgaagcc catattgaag
    20221 gcataggttc agttgctgcg gcattttcaa gcgcagcccc aaaaggaagt ctgtcatgag
    20281 taagcgtaaa gtcgccatta tcggttctgg caacattggc accgatctga tgattaaaat
    20341 tttgcgtcac gatcagcatc tggagatggc ggtgatggtc ggcattgatc ctcagtccga
    20401 cggtctggcg cgtgccagac gtatgggcgt cgccaccacc catgaagggg tgatcgggct
    20461 gatgaacatg cctgaatttg ctgatatcga cattgtattt gatgcgacca gcgccggtgc
    20521 tcatgtgaaa aacgatgcgg ctttgcgcga agcgaaaccg gatattcgct taattgacct
    20581 gacgcctgct gccatcggcc cttactgcgt gccggtggtt aacctcgagg agaacgtcga
    20641 tcaactgaac gtcaacatgg tcacctgcgg cggccaggcc accattccaa tggtggcggc
    20701 agtttcacgc gtggtgcgtg ttcattacgc cgaaattatc gcttctatcg ccagtaaatc
    20761 tgccggacct ggcacgcgtg ccaatatcga tgaatttacg gaaaccactt cgcgagccat
    20821 tgaagtggtg ggcggcgcgg caaaagggaa ggcgattatc gtgcttaacc cagcagagcc
    20881 accgttgatg atgcgtgaca cggtgtatgt attgagcgac gaagcttcac aagatgatat
    20941 cgaagcctca atcaatgaaa tggctgaggc ggtgcaggct tacgtaccgg gatatcgcct
    21001 gaaacagcgc gtgcagtttg aagttatccc gcaggataaa ccggttaatt tgccgggcgt
    21061 agggcagttc tccgggctga aaacggcggt ctggctggaa gtcgaaggcg cagcgcatta
    21121 tctgcctgcc tatgcgggca acctcgacat tatgacttcc agtgcgctgg cgacagcaga
    21181 aaaaatggcc cagtcactgg cgcgcaaggc aggagaagcg gcatgaacgg taaaaaactt
    21241 tatatctcgg acgtcacgtt gcgtgacggt atgcacgcca ttcgtcatca gtattcgctg
    21301 gaaaacgttc gccagattgc caaagcactg gacgatgccc gcgtggattc gattgaagtg
    21361 gcccacggcg acggtttgca ggggtccagc tttaactatg gtttcggcgc acatagcgac
    21421 cttgaatgga ttgaagcagc ggcggatgtg gtgaagcacg ccaaaatcgc gacgttgttg
    21481 ctgccaggaa tcggcactat tcacgatctg aaaaatgcct ggcaggctgg cgcgcgggtg
    21541 gttcgtgtgg ctacgcactg taccgaagct gatgtttccg cccagcatat tcagtatgcc
    21601 cgcgagctcg gaatggacac cgttggtttt ctgatgatga gccatatgac cacgccggag
    21661 aatctcgcaa agcaggcaaa gctgatggaa ggctacggtg cgacctgtat ttatgtggtg
    21721 gattctggcg gtgcgatgaa catgagcgat atccgtgacc gtttccgcgc cctgaaagca
    21781 gtgctgaaac cagaaacgca aaccggcatg cacgctcacc ataacctgag tcttggcgtg
    21841 gcgaactcta tcgcggcggt ggaagagggc tgcgaccgaa tcgacgccag cctcgcggga
    21901 atgggagcgg gcgcaggtaa cgcgccgctg gaagtattta ttgccgccgt ggataaactg
    21961 ggttggcagc atggggccga tctctatgcg ttaatggatg ctgctgacga cctggtgcgt
    22021 ccgttgcagg atcgaccggt acgagtcgat cgcgaaacgc tggcgctggg atacgctggt
    22081 gtttactcca gcttcctgcg tcactgtgaa acggcggcgg cgcgttatgg cttaagcgcg
    22141 gtggatattc tcgttgagct gggcaaacgc cggatggttg gcggccagga ggatatgatc
    22201 gtcgacgtgg cgctggatct gcgcaacaac aaataatatt gactgccgag aacgtgcatt
    22261 ttgtatgccc atcccagcac gactgccgga tgcggcgtga acgccttatc cggtctacat
    22321 ttcgcccgta ggcagtcatt aaatagttct gattacgcgg ctggcaatgt atcagtcgcg
    22381 attcacctgc acacgcaatg aggttcatct tatgtcgact cgtacccctt catcatcttc
    22441 atcccgcctg atgctgacca tcgggctttg ttttttggtc gctctgatgg aagggctgga
    22501 tcttcaggcg gctggcattg cggcgggtgg catcgcccag gctttcgcac tcgataaaat
    22561 gcaaatgggc tggatattca gcgccggaat actcggttta ctacccggcg cgttggttgg
    22621 cggaatgctg gcggaccgtt atggtcgcaa gcgcattttg attggctcag ttgcgctgtt
    22681 tggtttgttc tcgctggcaa cggcgattgc ctgggatttc ccctcactgg tctttgcgcg
    22741 gctgatgacc ggtgtcgggc tgggggcggc gttgccgaat cttatcgccc tgacgtctga
    22801 agccgcgggt ccacgttttc gtgggacggc agtgagcctg atgtattgcg gtgttcccat
    22861 tggcgcggcg ctggcggcga cactgggttt cgcgggggca aacttagcat ggcaaacggt
    22921 gttttgggta ggtggtgtgg tgccgttgat tctggtgccg ctattaatgc gctggctgcc
    22981 ggagtcggcg gttttcgctg gcgaaaaaca ggctgcgcca ccactgcgtg cgttatttgc
    23041 gccagaaacg gcaaccgcga cgctgctgct gtggctgtgt tatttcttca ctctgctggt
    23101 ggtttacatg ttgatcaact ggctaccgct gcttttggtg gagcaaggat tccagccatc
    23161 gcaggcggca ggggtgatgt ttgcactgca aatgggtgcg gcgagcggga cgttaatgtt
    23221 gggcgcattg atggataagc tgcgtccagt aaccatgtcg ctactgattt atagcggcat
    23281 gttagcttcg ctgctggcgc ttggaacggt gtcgtcattt aacggtatgt tgctggcggg
    23341 atttgtcgcg gggttgtttg cgacaggtgg gcaaagcgtt ttgtatgccc tggcaccgtt
    23401 gttttacagt tcgcagatcc gcgcaacagg tgtgggaaca gccgtggcgg tagggcgtct
    23461 gggggctatg agcggtccgt tactggccgg gaaaatgctg gcattaggca ctggcacggt
    23521 cggcgtaatg gccgcttctg caccgggtat tcttgttgct gggttggcgg tgtttatttt
    23581 gatgagtcgg agattaagaa tgcagccgtg tgccgatgcc tgacgcgcct tattaagcag
    23641 gggcgaaagg gacttgcccc caatacaccc acggcgttta caggtatact cgctaaaaat
    23701 tattcagcgg gtttggaaac aaagatggca aaacttacct tacaagagca gttgctcaaa
    23761 gcaggattag tcaccagcaa aaaagcggcg aaggtcgaga gaacggcgaa aaaatcgcgc
    23821 gttcaggcgc gtgaagctcg ggcggcggta gaagaaaata aaaaggcaca gcttgagcgt
    23881 gataaacagc ttagcgaaca gcaaaaacaa gcggcgttgg cgaaagaata taaagctcag
    23941 gtgaagcagc ttattgaaat gaaccggatc accattgcca atggcgatat tggttttaac
    24001 ttcactgatg gcaatctgat taagaagatt tttgtcgata agctcacaca ggcgcagttg
    24061 attaatggtc gtctggcgat tgcccgcttg ttggtcgata acaatagcga aggtgaatac
    24121 gccattattc ccgccagcgt tgccgataaa attgctcagc gtgatgccag cagtattgtg
    24181 ttgcacagcg cgctcagtgc cgaagagcag gatgaagacg acccgtatgc cgacttcaaa
    24241 gtgcctgatg atttgatgtg gtaaacatag ataagtcgtg cgcagatgcc tgatgcgacg
    24301 ctatgcgcgt cttatcatgc ctacggcctg cgggcgaaat gtaggcggat aaggcgttca
    24361 cgccgcatcc ggcagtcgtg cactattatc aacgcatatt cagtttattg gcgtggtagg
    24421 caatatgctc gccaataaaa ctggagacaa aataatagct gtggtcgtaa ccctcgtgat
    24481 aacggattaa cgttttgata ttcatctcct ggcagatctt ttcaagattt tgagtccgca
    24541 gctgttccgc gtaaaaatca tcactcaacc cctgatcaac catgatttcc gcaacgcgtt
    24601 gaccttgtga aataagactc accggatcgt aatccaacca ggcctcttta ttttcaccaa
    24661 gatatgcggc aaaggcttgc tgtccccacg gtacttgcga tggggagaca atgggcgaaa
    24721 acgccgagac gctgacatat tcatctgggt tacgtaacgc cagcaccagc gcgcccagtc
    24781 cgcccataga atgaccagag atagactttt tggccgttgc cggaaaatga ttcatcacta
    24841 aatccggcag ctcgttgcgg atatagtcat acattttgta atgttcattc cacggcgctt
    24901 gcgtcgcgtt caggtaaaac ccggcacctt gcccgagatc gtaacggtcg gcatctgcga
    24961 catgactgcc tcgcggactg gtgtccggcg caacaacaat aatgttgtgc tcagccgcgt
    25021 aacgctgcac ccccgattta gtaatgaaat tctgctcatt gcaggtcagg cccgaaagcc
    25081 agtacagcac cggtaatttt tcacctgcag cttttggtgg gagatagacg ccgacattca
    25141 tttcacattt cagtgatgcg gaataatgcc gatacacatt ttgccagccg ccaaagctgg
    25201 catgtttttc aatgagttcc atcagtcatt atctcacggt taatcccccg gacggctgcc
    25261 cggagagagt attaagaacc ccgtctgaag tggacggggt aaaccagcgg gaaatcagta
    25321 acgaattacg gttcgaatgg atttgccttc atgcatcagg tcgaaggcgt cattaatttc
    25381 atccaggctc atggtatgcg tgacaaacgg ttccagatcg atatcacctt tcatcgcatc
    25441 ttcaaccata cccggtaact gggaacgacc tttcacgccg ccaaacgcgg aacctttcca
    25501 tacgcgacca gtgaccaact ggaatggacg ggtggagatt tcctgaccgg aacccgcgac
    25561 cccgatgatc accgactgac cccagccgcg gtgcgcactt tccagcgccg cacgcatcac
    25621 gttgacatta ccgatgcatt caaaggtatg gtcgataccc catttgttga tatccagcag
    25681 gacatcttta atcggtttgt cgtagtcatt cgggttaatg cagtcggtgg caccgaagcg
    25741 acgcgccaga tcgaatttct tcgggttggt atcgatagcg ataatccgtc ccgctttcgc
    25801 ctgacgcgcg ccctgaacca ctgccagacc aatcgcgcca agaccaaaca cagcaacaga
    25861 atcccctggc tggactttag ctgtgttgtg taccgcgcca ataccggtgg tcacgccaca
    25921 gcccagcagg cagacgtgtt catggtttgc ttctggatta atttttgcca gagacacttc
    25981 cgcgactacg gtgtattcac tgaatgtaga acaccccatg tagtgataaa gcggctgccc
    26041 attgtaagaa aaacgggtgg tgccgtctgg catcaggcct ttaccctggg tttcgcgaac
    26101 cgcaacacag aggttagttt tgccagaacg acagaactca cactcgccgc actctgcggt
    26161 gtaaagcggg atcacatggt cgcctggttt gacgctggtt acgccttcac cgacttcaac
    26221 cacaacgcca gccccttcgt gaccgagaac caccgggaat acaccttccg ggtcatcgcc
    26281 ggagagggta aatgcgtcgg tatggcaaac gccggtatgg gtgactttaa tcagcacttc
    26341 accttttttc ggtggtgcaa cgtcaatttc aacgatttcc agcggtttac cgggagcaaa
    26401 tgcaacggca gcacgtgatt tcatctctcg ctcttcctca atatggtaat agattcagct
    26461 atttaagata ggcacgaacc agttcaatag tgtcgtcaac ggattggctg acttcgcggc
    26521 tgtagcagtc atttcggtca aacgtttccc ggatatggct ttcaagcact tctgccatca
    26581 gcccattagc cgcgccccga acggcagcga tctgttggag tatggcacgg cattcggcat
    26641 caccctccag cgaccgttcc agagcatcaa tctgccctcg aatacgacga actcgagtaa
    26701 ggaccttttt cttctcttcc ggagtactgg gcatttcgca cctcatcatc tgcacgcaat
    26761 atactatagg ggggtatgct atatgtcaat gcataccccc ctatagtata taacaatgga
    26821 gaataattgt tgtccgaagc ctaaccaggt gaacggtccc tcaagacaga tggggaccgt
    26881 ttatgtgcta tttttatgcc gcatccgcta aaacaaacat cccgtaagga tggatttcga
    26941 ggtaatagtg ctcaccaata tcgggctgta aacgggtggc gtttacttgt aataaaatct
    27001 cctgtccatg ccactctacg gtcacttcgt actgcggccc catgtaggcg acatggcgaa
    27061 taacacaacg ctgactctct tcgccgtgat tgcttaaggt gatggcttcc gggcgaacgc
    27121 cgacggtgcc gctaccttgc accgggaaat gcgccgcgcg cggcaggcga taaccgtaga
    27181 tatcgacata ctcttcgcta aagttcgccg ggaacaggtt ggcgtcgccc ataaaacttg
    27241 ccataaagcg ggaggctggc tgacgataga gatcctgcgg tgagccaatc tgcatgatgt
    27301 gacctttatt catcaccagc acagtgtcgg aaaccgcgaa ggcctcactt tgatcgtgag
    27361 tcacgtagag cgacgtgata ttaaactgct tttgcagctc gcggatctta tcgcgcatgc
    27421 tgcggcgcag gttggcatcg aggttactta acggctcatc aaacagcagc acctttggtt
    27481 tgaggatcaa cgcgcgggcc agcgccacgc gctgttgctg accaccggaa atctggtcga
    27541 catagcggtc ctcgaaccct tccagatcca ccattgccag cgcctctttc acccgctgct
    27601 ttacttcgct acgcgacacg ccgagcatct ttaacccgta gccgacgttt tcgcccagcg
    27661 acatatgcgg gaacagagcg tatgactgaa acaccatgca gatatcgcgc tgctgaatgg
    27721 aacgatgcgt gacatcttcg ccatcaataa agatttgccc ttcactcggt ttttccagcc
    27781 cggcaaccag acgcaaaatg gtggttttgc cgcagccgga agggccaagc agcgtcacca
    27841 tttgcccttg tgggatggtg agattgatat tgtcgataac cgtattactg ccgaatcgtt
    27901 tagtgacgtt gcgcagttcc acaaaatttt tctgactcat agtgcgctcc attacgcctg
    27961 gtttttggct ttagaacggg agatacgtgc ttctccgatc agccagtcaa agaggaaaat
    28021 aatcgccagc atcaccacga tcagaatcga accataggcg atggctacgc catattcgcc
    28081 gtcttcaacg cggttaagaa tgtaggcggt tgccacgcgg gtgtccggcg tgacgagaaa
    28141 gacgatggcg ctgaccgtcg tgatggcccg cacaaagctg tagatcaggg ccgagagaat
    28201 ggccggacgc agcagcggca gtaaaatgtg ggttatggtt cgcaaggaac ccgcgcgcag
    28261 gctgagtgag gcttcatcca gcgatttgtc gatctgcccc aggcctgcaa tcccggcgcg
    28321 aatacccacc ggaacgttac gcatcaccat cgagataatc acaattgccg ccgttccggt
    28381 gagataaacc ggagcgctgt taaaggcaag aatatacgaa acgcccgcca ccgtacccgg
    28441 tacggcaaag cacagcatgg tggtgaactc gatggtcttt ttgcctttga attgttgacg
    28501 caccaccacc caggcgatga gcaggccaaa gatagcggta atgggagcgg caatcccggc
    28561 gtagagcagg gtatccagca gtgaaggcca cgcgccgtcg ctcattccct gaccaaatag
    28621 cttgataaag ttatccagcg tcagggtgta gtccacgccc cagttcaccg tgaagctgcc
    28681 gtagaaaatg ctgccgtaga gcagggcgtt aaaggcaatc catatcgcaa gtatggcgac
    28741 cacgctccac accagggtga ccggaagcgg ctggacgtcg ccacggtagg atttgccgga
    28801 aaccgtgacg taagagcgtt taccaatcca catatactgt atgcagaaca ccagcagcga
    28861 gaacagcagc aggaaagcgc caagggtgct ggcggcctga taatccagct gcgagccggt
    28921 aatatagaag taaatttgcg tcgccagtac gtcgaagtta ccgccaagta ccagcgggtt
    28981 actgaaatca gccagtgact gtaccacgac gattaaaaag gcattggcga gcgcgggctt
    29041 caacaacggc acaaacacgc cgttaaaggt ttgccagcgg ctggcgcgca gggtgtagga
    29101 ggcctcttcc agcgagggat gaatggtttt aatggccccg tcgaggatca taaatgccat
    29161 tggcgtgaat gccagaacct gtgccagcca gatcccggta aaaccgtaca gccagttggt
    29221 gttggtcagc ccaaaccact ccaccataaa ttccgtaaca tagccggaac gccccatcat
    29281 gagcgtcacg cctaagccga ccacaaacgg tggcgtaacg atgggcaaaa tggaaaagat
    29341 gcgaccaata atggcgctgc gtttagcgat gcgcgtggta taaatcgcca gcaccaggcc
    29401 gaagaaagca cagcctatcc ccaccgctat cgacaagacg atagagttga cgatcacctg
    29461 aacaatatgt gcctgcgaga gcacggtcat aaacgccagc ggggcgagct ctcccgcgtc
    29521 gttggtgaac atcgggataa aaatggcgat gctcggccag acgataaaaa tgccaatcag
    29581 cgccacaatc gcaatcagtg agccgatcac gaagcggtcg ccgcccagcc attcgaggcg
    29641 ggttaaggcc agcgtcataa tcgcgcctag ttcgacaaac agcacgatgg cggcgtagcc
    29701 taaaccgcgt ccagccacgg tggcgctgat gacgataaac gccatacaga gcaatgccca
    29761 gccggcatcg aaataatgac gactacgctg ttcgcgcgtg gcctgacgaa acgggcggac
    29821 cagcaacagg ctcgggagta aataccacag ccagctgata ttaaaatgcg accagccgta
    29881 cgccccgagg atctcatcac tcgtggattc cagcaggcca taatcgaggc tccagctcgg
    29941 caacaggaca aatgccagcc agcccagcaa aacccaaagg aaaatcgcat cccgcttttt
    30001 cacgggatgg agtgcaagtg tctgtgacat agcagttccg gtatggttgg ccggggcagg
    30061 cgtctgcgct gcacccggca aatgcgccta atgcgggttt atttacccat cttcacttcg
    30121 ctgacccatt tattaatcaa tgccttacga acatccgtgg aaccatattt atccatgtcg
    30181 tagttgatta atttcaggtc gtcgagcttc agcgaatttg gcgacgtttc ggcggtggta
    30241 ttagtcagga tctgataaga cttccctttc ttccacgcca gttcctgagc ttcttttgat
    30301 aatacccagt ccacaaacag tttggcgttg tcgaggttgc gcgcgccttt cagaatgctg
    30361 acgccgccaa tttcgtagcc cgtaccttca cacggggaaa tcaactccag cggcgcgccc
    30421 tgttcttttt ccagcgagta gtcatgcagg aagccaatac caatcgccgt ttcgccacgg
    30481 gcggcgttac gtgccggggc tatgcctgat ttggtgtact gagagacgtt ggcgttcagt
    30541 tgttttagat aatcaaaggc ctgatcttcc ccccacagct gcgcaaaagt agccagggcg
    30601 gtgtaggcag taccggaact ttgcggatcg gcaatttgaa tttcgccttt atattccggt
    30661 ttggtgagat ctttccagca cttcggcacc ggcaggtttt tctctttcag acgatcggtg
    30721 tttacgccaa aaccgaggat ccccacataa acggcagagg acagattccc tttcactttt
    30781 gccggatcgc ggaatttctc catgatctgc tcaaggttag gggatttata cgcctgtagc
    30841 aggcccatct cgccagcctg cgattgcggg tctaaagtgc cgccgtacca gacgtcagcc
    30901 tgggggtttt tcttttcagc atcgactttt gccagggtgc ttccggaacc gttgcggata
    30961 aacgaggttt ttacatcata tttctcgcca aacgctttgg tttcggcctc acacatttca
    31021 ttggtggcgc tgcaatagac caccagccgg ccttttgcct gcgcggcacc cgttaaagtc
    31081 gccagcgcaa cggcagaagc aatcagagta gaggtaaagg tcattttcat tgttttatcc
    31141 ttcgagcgtg agtggtggtg atgcccgcca tcagtaacgg catcagcagt aaacccataa
    31201 gtactgcggc gaccgagagt aaactgaaca ttcccggcca gccgtagcgt tcgatgacca
    31261 gcgacagcgg ccagcctgcc agcgccgcgc caaggtaagc gaacaggccg agaaatccgg
    31321 tgattgaacc cgccgcagct ttgtgcccac actccaccgc cgcaagtcca atcagcattt
    31381 gtgggccaaa gacgaaaaag cccacggtaa aaaagcacac tgccagcaga gcgtagtgat
    31441 gaaccggtgc cagccacagt gcggcgactg acaccatcaa ccccaacgtg aacagcaaaa
    31501 tcattggcgc tcgttgcccg ctaaacagca aatccgatcc ccatccggca aataacgcgc
    31561 cgagtaagcc gcccacttca aacagcatta ccgtggcgtt ggcgctgagc aggttgacac
    31621 cgtggctttc cgtcagccag atattgcccc agtcgttaag cgcaatccgg atcacataaa
    31681 ccagtacata tgagacgccc agtagccaga tcagcggatt ttgcagcatg gtggtgcgca
    31741 acatttgcca cagccccatc ggcggacttt gttgttcctg acgtaactcc agcggatcgt
    31801 gacgccagtg accaactgtc ggtaatccct cttcctgggg cgtgcctttg agttgtagcg
    31861 ttaaccagat ccctaatgcc atgctgatta tccccggtgt cagcatcgcg gcctgccagc
    31921 cccaccagtg ggcggcaaag gcgctaatca gcggaataat cgctccgcca atgttgatcg
    31981 acatattcca gcagccccac cagaaaccgc gctcattgcg tgagtaccag tgggtcagca
    32041 agcgggcaca cggtggccat ccccagccct gaaaaaaacc gttcagtgtc cagaccacaa
    32101 gcagaagcgt aagtgattcg ccaaaggcaa acaccacgtt caataacccg gtggcgaaca
    32161 agccgacgcc cataaaaccg cgttgtccgt ggctgtcatg ccacagtccg gcggtaaatt
    32221 tcgacaatcc gtagctgaga taaaacagcg aacccagcag gccaatgtcg cctttatcca
    32281 gtccgaggtc ggtttgtagc gccggaagga cgtaattcac gctttttcgc gtcagataaa
    32341 atgcggcgta gccaataatc atgcacatca gcaatcgcgg acgtaatgtc cggtagcatt
    32401 gattaatttc tctcgctgaa cgcgcgtgca tggttgtctc catgttgctg actatatgaa
    32461 aatgaaagtg aaaggggatg agagaaaatc cgggatacct aagacttttt cctggttatc
    32521 cggcgatttg ttgcaaaaat gtgggcaagt taacaattac ccgcgtgccg tgctgttttt
    32581 cgagcgttaa gtcgccgccc agggcgctga cgcgctcacg catcccctga atgccaaatc
    32641 cagccatttt cccgctggcg atccccacac cgttgtcgct gacttccaga tgcaatacct
    32701 ctccctgctg gcgcaggata atcgtcactt cgctggcttc ggcgtgttta cagatgttat
    32761 tgagcaactc ctgcaataac cgatacaagg taaagcgcac cgtttcgttt tctggtgtgg
    32821 agttcagttg ataggcaaac tggcaatgaa tgccgcgttc actaaaggca aattcattaa
    32881 tcagatgcag caacgcttcg cgaaatgtca gttcatcaag cgcgggaggc cgcaactggc
    32941 gcagcaattg gcgggtggag agatgaatgc ggcgggcgag ctcattgatt tggctggctg
    33001 cggattgaat ttgcgccgga tcacgcgccc gcttcaccag ttgcgactgg atctgaatgg
    33061 cggtgatgtt ctgaccgatt tcgtcgtgca gctctcgcgc caggtttttt cgcgtgtcct
    33121 cctcggtgtg gatcagcttt tccgccaatg cccgccgcgc tgccagctcg gcctccagcc
    33181 gttgtcggta atggtgcagg tttagcgcca gatgttgctg gcgactgatg gcaatgccca
    33241 ggccaattcc cagcaaggcc tgggtcgcga ggaaaatctc cagctccacc aggttactaa
    33301 agcccacgcc aatctggcgg gcgatggtga tcatcatgct gcccaaaagc ccggagagta
    33361 cgccaccttg ccagccaaat ttccacgcca tcaccacatt gggcaaaaag accacgatca
    33421 acagcagacg ttcaatttca ggtgacagca ccatctgagt gccgatacca ataataaaaa
    33481 acaggctaca ccagatgatt aaagaggtac gcaagggggg attatgcgta tcgagtccca
    33541 ggagctggta gcgatgctgc tggcgcagaa actcaaagat aagatagaca aacggcgtca
    33601 gtaatacgcc gccggtaaat gaggtcaaac ccggtagcat tattgggcct gacataaaag
    33661 gggagagcag ggcggtattc aacaatgaag ctgccgtgac agtggctaac agcaacgtca
    33721 gacgctgcca gtaaagtgga aaacggtgcc agaagttttg cgcaaggagg gcaggaaaca
    33781 ggctaataac cggcgttgcc agcatgagat aaccgttcaa tagctgttca ctatggagcc
    33841 agaacatcat cagcaaaggg gggagcacca acgcaggcca gtagcggcga gagagcaaaa
    33901 taacaagggc gagataaact ccctgcggga gaaacaacac cgcctgttga ccgttattgg
    33961 tcagataaaa gctgagcgtc cacagcatca gccagccggt tccccaggct agcacaataa
    34021 acagcaaaat cacccaatga cgaagattgc gcgacattaa tgccccgcca gcagatggtg
    34081 gtcgagcgca aaatgtacca gttcaatggt gctatggcag ttcagttttc ccagcacatt
    34141 ggcgcgatgc acatgtaccg ttttatgact gagttcgagt ttaaaggcga tctctttaac
    34201 gctgtctcct ttgaccagta actcaaacac ttcacgttct cgtggggtga gaatttccag
    34261 cgcctgggcg ggttgcccgc cgccgcgtaa agcccgaatc gcatcggcac agagataatg
    34321 cccgcccagc ccgaccgagc gcaccgcctg tactaactct tccggcccac agcgtttggt
    34381 taaatacccg ctggcaccgg catccagcgc gctttgcaca aaggttggcg cgtcataaat
    34441 gctaagaata atggcgcgaa attgcggttt ttgtgcacgc aggcgcttaa gcaaactgag
    34501 gccgttttcg tccggcatgg caatatcaat gacggcaaca ttgacgtcat cacggattaa
    34561 tgccgaccat gcctgcgcgg cactactgta ctgaccaatg acctccagat cgtcttccag
    34621 agagagtaat tgtgcgaagc cagagcgcac cacaacatgg tcatccacca gcactacccg
    34681 aatcatactt ttcttctctt attatctaat tctcctatca tgcatcacgg cgcggcgctg
    34741 gcaatcaacc agcgacagat atggaatgga tgtaacaaaa tggcaataat ttgccggagc
    34801 gcgcacatct tatctggctt gtgaaccggc gggatacgtt cgccaaaacc gcaaaatgcg
    34861 caaactcgct aaccattgcc gatttttttc taaaaacgct ttgttcttaa caagaccttt
    34921 taattccttt atcaaactga cccgcaacga aatactgctc ccataacaag acaggggagc
    34981 agacaatcat ggcaatttca tcgcgtaaca ctcttcttgc cgcactggca ttcatcgctt
    35041 ttcaggcgca ggcggtgaac gtcaccgtgg cgtatcaaac ctcagccgaa ccggcgaaag
    35101 tggctcaggc cgataacacc tttgctaaag aaagtggagc aactgtggac tggcgcaagt
    35161 ttgacagcgg agccagcatc gtgcgggcgc tggcttctgg cgacgtgcaa atcggcaacc
    35221 ttggttccag cccgttagcg gttgcagcca gccaacaggt gccgattgaa gtcttcttgc
    35281 tggcgtcaaa actgggtaac tccgaagcgc tggtggtaaa gaaaactatc agcaaaccgg
    35341 aagatctgat tggcaaacgc atcgccgtac cgtttatctc caccacccac tacagcctgc
    35401 tggcggcgct gaaacactgg ggcattaaac ccgggcaagt ggagattgtg aacctgcagc
    35461 cgcccgcgat tatcgctgcc tggcagcggg gagatattga tggtgcttat gtctgggcac
    35521 cggcggttaa cgccctggaa aaagacggca aggtgttgac cgattctgaa caggtcgggc
    35581 agtggggcgc gccaacgctg gacgtttggg tggtgcgcaa agattttgcc gagaaacatc
    35641 ctgaggtcgt gaaagcgttc gctaaaagcg ccatcgatgc tcagcaaccg tacattgcta
    35701 ccccagacgt gtggctgaaa cagccggaaa acatcagcaa actggcgcgt ttaagcggcg
    35761 tgcctgaagg tgacgttccg gggctggtga aggggaatac ctatctgacg ccgcagcaac
    35821 aaacggcaga actgaccgga ccggtgaaca aagcgatcat cgacaccgcg cagtttttga
    35881 aagagcaggg caaggtcccg gctgtagcga atgattacag ccagtacgtt acctcgcgct
    35941 tcgtgcaata aaaggaggcg cagatgctgc aaatctctca tctttacgcc gattatggcg
    36001 gcaaaccggt actggaagat atcaacctga cgctggaaag cggcgagcta ctggtggtgc
    36061 tggggccgtc cggctgcggt aaaaccaccc tgctgaattt gattgccggt tttgtgcctt
    36121 atcagcatgg cagcattcaa ctggcgggta agcgtattga ggggccggga gcagagcgtg
    36181 gcgtagtttt tcagaatgaa gggctactac cgtggcgcaa tgtacaggac aacgtggcgt
    36241 tcggcctgca attggcaggt atagagaaaa tgcagcgact ggaaatcgcg caccagatgc
    36301 tgaaaaaagt ggggctggaa ggcgcagaaa aacgctacat ctggcagctt tccggtggtc
    36361 aacgtcagcg ggtggggatt gcccgcgcgc tggcggcgaa tccccagctg ttattactcg
    36421 acgaaccgtt tggtgcgctg gacgccttca cccgcgacca gatgcaaacc ctgctgctga
    36481 aactctggca ggagacgggc aagcaggtgc tgttgattac ccacgatata gaagaagcgg
    36541 tgtttatggc gactgaactg gttctgcttt cacccggccc tggccgcgtg ctggagcggc
    36601 tgccgctcaa ctttgctcgc cgctttgttg cgggagagtc gagccgcagc atcaagtccg
    36661 atccacaatt catcgccatg cgcgaatatg ttttaagccg cgtatttgag caacgggagg
    36721 cgttctcatg agtgtgctca ttaatgaaaa actgcattcg cggcggctga aatggcgctg
    36781 gccgctctcg cgtcaggtga ccttaagcat tggcacgtta gcggttttac tcactgtatg
    36841 gtggacggtg gcggcgctgc aactgattag cccgctgttt ttgccgccgc cgcaacaagt
    36901 actggcaaaa ctgctcacca ttgccggacc gcagggattt atggacgcca cgctgtggca
    36961 gcatctggca gccagcctga cgcgcattgt gctggcgcta ctcgcggcgg tgttgatcgg
    37021 cattccggtc gggatcgcca tggggctaag cccgacggtg cgcggcattc tggatccgat
    37081 aatcgagctt tatcgtccgg tgccgccgct ggcttatttg ccgctgatgg tgatctggtt
    37141 tggtattggt gaaacctcga agatcttact gatctattta gcgatttttg caccggtggc
    37201 gatgtcggcg ctagcggggg tgaaaagcgc gcagcaggtt cgcattcgtg ctgcccagtc
    37261 gctgggtgcc agccgtgcgc aggtgctgtg gtttgtcatt ttgcccggcg cgctgccgga
    37321 aatcctcacc ggattacgta ttggtctggg tgtgggctgg tctacgctgg tggcggcgga
    37381 gctgattgcc gcgacgcgcg gtttaggatt tatggttcag tcagcgggtg aatttctcgc
    37441 gactgacgtg gtgctggcgg ggatcgcggt gattgcgatt atcgcctttc ttttagaact
    37501 gggtctgcgc gcgttacagc gccgcctgac gccctggcat ggagaagtac aatgagtgaa
    37561 cgtctgagca ttaccccgct ggggccgtat atcggagcac aaatttcggg tgccgacctg
    37621 acgcgcccgt taagcgataa tcagtttgaa caactttacc atgcggtact gcgccatcag
    37681 gtggtgtttc tgcgcgatca agccattacg ccgcagcagc aacgcgcgct ggcccagcgt
    37741 tttggcgaac tgcatattca ccctgtttac cctcatgccg aaggggttga cgagatcatc
    37801 gtgctggata cccataacga taatccgcca gataacgaca actggcatac cgatgtgaca
    37861 tttattgaaa cgccacccgc aggggcgatt ctggcagcga aagagttacc ttcgaccggc
    37921 ggcgatacgc tctggaccag cggtattgcg gcctatgagg cgctctctgt tcccttccgc
    37981 cagctgctga gcgggctgcg tgcggagcat gattttcgta aatcgttccc ggaatacaaa
    38041 taccgcaaaa ccgaagagga acatcaacgc tggcgcgagg cggttgcgaa aaatccgccg
    38101 ttgctacatc cggtggtgcg aacgcatccg gtgagcggta aacaggcgct gtttgtgaat
    38161 gaaggcttta ctacgcgaat tgttgatgtc agcgagaaag agagcgaagc cttgttaggc
    38221 tttttattcg cccatatcac taaaccggag tttcaggtgc gctggcgctg gcaaccgaat
    38281 gatattgcga tttgggataa ccgtgtgact cagcactatg ccaatgccga ttacctgcca
    38341 cagcggcgga ttatgcatcg ggcgacgatt cttggggata aaccgtttta tcgggcgggg
    38401 taatacgaga gtggacggtc ctctcgcccc cttggggaga gggttagggt gagggggcgt
    38461 tcaccgtgct ttcaacaggt taactccccc tttctgagag gaaacaaaat taacgcagaa
    38521 tcttcttctc agccaaatcc agcgcaaagt agctgaaaat cagatccgca cccgcacgct
    38581 taatcgaacc taagctttcg agcacgactt tctcttcatc tatagcaccc gccagcgcgg
    38641 cgaacttaat catcgcatac tcaccgctca cctgatacgc gccaatcggc aattcagtac
    38701 gttcacgcag ctcacgcacg atgtcgaggt acgctccagc aggtttaacc atcaggcagt
    38761 ctgcgccctg ggcttcatcc agcagtgact cacgaatcgc ctcacgacgg ttcattgggt
    38821 tcatctgata gcttttgcgg tcgcctttta atgcgcttcc ggcagcttca cggaacggac
    38881 cataaaagga ggaggcgaac ttggtcgaat acgacataat cgccgtatct ttaaagccag
    38941 cagcgtccag cgcctggcga atcgcctgta cctggccatc catcgcggcg gaaggggcga
    39001 tgaagtctgc acctgcagca gctgcaacca cggcttgctt gcctaaattt tccagagtcg
    39061 cgtcgttgtc gacgccatgc tcgcacagca caccgcagtg accgtgagaa gtgtattcac
    39121 agaagcaggt gtctgacatg acaatcattt ctggcaccgt ctgcttgcag atgcgcgaca
    39181 tacgcgctac cagtccatct tcccgccagg catcgctgcc ggtttcatcg gtatggtgag
    39241 agatgccgaa agtcatcacg gaacgaatac cggcgttggc gatgcgttca atttcgcgtg
    39301 ccagatgttt ctctggaatg cgcatcacgc caggcatggc ttcaacggct ttgtagtcgt
    39361 caatttcttc ttcaacaaag atcggcaaca ccaggtcgtt aaggctaagt gttgtctctt
    39421 caaacatagc gcgcagcgca ggagatttgc gcaggcgacg agggcgttgg attaagtctg
    39481 tcatggtctg cctgatgttt gtggaattaa ggtgcacagt atacctgaag cggggctgga
    39541 gtgttttacg aaagttgtca cgatgttgac aagaagagaa tgaaagagag accagggcat
    39601 ctttgtaata gaaatagcaa taacttcata ttcactgaaa atatttttta atttttattt
    39661 atag
//
LOCUS       DAZFAB010000034        39220 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZFAB010000034 DAZFAB010000000
VERSION     DAZFAB010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39220
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.034"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..614)
                     /gene="yncG"
                     /locus_tag="ACHJWA_002051"
     CDS             complement(<1..614)
                     /gene="yncG"
                     /locus_tag="ACHJWA_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFO4308649.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQVPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWASDAPEQLKKNVIEYRKSLYVWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNATNISAIADAVCQLPTLQEILKRNEI"
     gene            881..2380
                     /gene="ansP"
                     /locus_tag="ACHJWA_002052"
     CDS             881..2380
                     /gene="ansP"
                     /locus_tag="ACHJWA_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFO4308650.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            complement(2495..3556)
                     /gene="yncE"
                     /locus_tag="ACHJWA_002053"
     CDS             complement(2495..3556)
                     /gene="yncE"
                     /locus_tag="ACHJWA_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFO4308651.1"
                     /translation="MHLRYLFSLRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKDSVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            3798..5900
                     /gene="pqqU"
                     /locus_tag="ACHJWA_002054"
     CDS             3798..5900
                     /gene="pqqU"
                     /locus_tag="ACHJWA_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFO4308652.1"
                     /translation="MKIVFVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDDASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSSRDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGSVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGMEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PAPGRNYGVGVNIAWRFE"
     gene            complement(5936..6601)
                     /gene="mcbR"
                     /locus_tag="ACHJWA_002055"
     CDS             complement(5936..6601)
                     /gene="mcbR"
                     /locus_tag="ACHJWA_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFO4308653.1"
                     /translation="MPGTEKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(6799..7836)
                     /gene="curA"
                     /locus_tag="ACHJWA_002056"
     CDS             complement(6799..7836)
                     /gene="curA"
                     /locus_tag="ACHJWA_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFO4308654.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQSGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQREMGQWVKEDKIHYREEITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            8017..8535
                     /gene="mddA"
                     /locus_tag="ACHJWA_002057"
     CDS             8017..8535
                     /gene="mddA"
                     /locus_tag="ACHJWA_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFO4308655.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            8532..8981
                     /gene="ydcZ"
                     /locus_tag="ACHJWA_002058"
     CDS             8532..8981
                     /gene="ydcZ"
                     /locus_tag="ACHJWA_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO4308656.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNSLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(8982..9215)
                     /gene="ydcY"
                     /locus_tag="ACHJWA_002059"
     CDS             complement(8982..9215)
                     /gene="ydcY"
                     /locus_tag="ACHJWA_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFO4308657.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(9301..9474)
                     /gene="ortT"
                     /locus_tag="ACHJWA_002060"
     CDS             complement(9301..9474)
                     /gene="ortT"
                     /locus_tag="ACHJWA_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFO4308658.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            9669..9764
                     /gene="yncL"
                     /locus_tag="ACHJWA_002061"
     CDS             9669..9764
                     /gene="yncL"
                     /locus_tag="ACHJWA_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFO4308659.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(9861..11285)
                     /gene="patD"
                     /locus_tag="ACHJWA_002062"
     CDS             complement(9861..11285)
                     /gene="patD"
                     /locus_tag="ACHJWA_002062"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFO4308660.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTAPSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTVACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTLFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(11307..12101)
                     /gene="ydcV"
                     /locus_tag="ACHJWA_002063"
     CDS             complement(11307..12101)
                     /gene="ydcV"
                     /locus_tag="ACHJWA_002063"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4308661.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLMALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(12091..13032)
                     /gene="ydcU"
                     /locus_tag="ACHJWA_002064"
     CDS             complement(12091..13032)
                     /gene="ydcU"
                     /locus_tag="ACHJWA_002064"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4308662.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            complement(13033..14046)
                     /gene="ydcT"
                     /locus_tag="ACHJWA_002065"
     CDS             complement(13033..14046)
                     /gene="ydcT"
                     /locus_tag="ACHJWA_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO4308663.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFICVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            complement(14064..15209)
                     /gene="ydcS"
                     /locus_tag="ACHJWA_002066"
     CDS             complement(14064..15209)
                     /gene="ydcS"
                     /locus_tag="ACHJWA_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFO4308664.1"
                     /translation="MSKTFARSSLCALSMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLQDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRDQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQTNALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASSL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            complement(15454..16860)
                     /gene="ydcR"
                     /locus_tag="ACHJWA_002067"
     CDS             complement(15454..16860)
                     /gene="ydcR"
                     /locus_tag="ACHJWA_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFO4308665.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKVPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQEYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWLWG
                     EREEQAVKQLGKLIQERL"
     gene            complement(16939..17355)
                     /gene="hicB"
                     /locus_tag="ACHJWA_002068"
     CDS             complement(16939..17355)
                     /gene="hicB"
                     /locus_tag="ACHJWA_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFO4308666.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            complement(17401..17577)
                     /gene="hicA"
                     /locus_tag="ACHJWA_002069"
     CDS             complement(17401..17577)
                     /gene="hicA"
                     /locus_tag="ACHJWA_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HFO4308667.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIK
                     EPLRKAILKQLGLS"
     gene            17799..18029
                     /gene="yncJ"
                     /locus_tag="ACHJWA_002070"
     CDS             17799..18029
                     /gene="yncJ"
                     /locus_tag="ACHJWA_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HFO4308668.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            complement(18121..20082)
                     /gene="rlhA"
                     /locus_tag="ACHJWA_002071"
     CDS             complement(18121..20082)
                     /gene="rlhA"
                     /locus_tag="ACHJWA_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFO4308669.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVVKVAKDHLDV
                     AVTEPLANGDGLNVLIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(20155..20691)
                     /gene="sutR"
                     /locus_tag="ACHJWA_002072"
     CDS             complement(20155..20691)
                     /gene="sutR"
                     /locus_tag="ACHJWA_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310064.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFO4308670.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVIISLFPYDPQLCFEHF
                     SIQMAPGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            20744..21955
                     /gene="ydcO"
                     /locus_tag="ACHJWA_002073"
     CDS             20744..21955
                     /gene="ydcO"
                     /locus_tag="ACHJWA_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFO4308671.1"
                     /translation="MLRCLYAITHEVTMRLFSVPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLLAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNR"
     gene            complement(21990..22658)
                     /gene="ydcL"
                     /locus_tag="ACHJWA_002074"
     CDS             complement(21990..22658)
                     /gene="ydcL"
                     /locus_tag="ACHJWA_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFO4308672.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(22962..23555)
                     /gene="tehB"
                     /locus_tag="ACHJWA_002075"
     CDS             complement(22962..23555)
                     /gene="tehB"
                     /locus_tag="ACHJWA_002075"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFO4308673.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGEYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWEMVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(23552..24544)
                     /gene="tehA"
                     /locus_tag="ACHJWA_002076"
     CDS             complement(23552..24544)
                     /gene="tehA"
                     /locus_tag="ACHJWA_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFO4308674.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTLALLMQGKLLVRTERAVLMKAEDKE"
     gene            24668..25648
                     /gene="ydcK"
                     /locus_tag="ACHJWA_002077"
     CDS             24668..25648
                     /gene="ydcK"
                     /locus_tag="ACHJWA_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFO4308675.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKNVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     INQGAYISDSVTIHDSLVCGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVIRYAFIEHRAEVFDFASIEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            complement(25643..26179)
                     /gene="rimL"
                     /locus_tag="ACHJWA_002078"
                     /pseudo
     CDS             complement(25643..26179)
                     /gene="rimL"
                     /locus_tag="ACHJWA_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
     gene            complement(26242..26466)
                     /gene="ydcH"
                     /locus_tag="ACHJWA_002079"
     CDS             complement(26242..26466)
                     /gene="ydcH"
                     /locus_tag="ACHJWA_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFO4308676.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDDRGY
                     NAEVVRMKKQKLQLKDEMLKILQHESVKEV"
     gene            complement(26606..28261)
                     /gene="opgD"
                     /locus_tag="ACHJWA_002080"
                     /gene_synonym="mdoD"
     CDS             complement(26606..28261)
                     /gene="opgD"
                     /locus_tag="ACHJWA_002080"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFO4308677.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            complement(28486..29829)
                     /gene="ydcJ"
                     /locus_tag="ACHJWA_002081"
     CDS             complement(28486..29829)
                     /gene="ydcJ"
                     /locus_tag="ACHJWA_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO4308678.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYDQRGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVAAQPITYEDFLPVS
                     AAGIFQSNLGNETQARSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            30046..30969
                     /gene="ydcI"
                     /locus_tag="ACHJWA_002082"
     CDS             30046..30969
                     /gene="ydcI"
                     /locus_tag="ACHJWA_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO4308679.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGPNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            complement(31007..32647)
                     /gene="trg"
                     /locus_tag="ACHJWA_002083"
     CDS             complement(31007..32647)
                     /gene="trg"
                     /locus_tag="ACHJWA_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFO4308680.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRTLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMKDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHYA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLNKQSVLAEPRGAGEPVSFAPV"
     gene            33046..33195
                     /gene="hokB"
                     /locus_tag="ACHJWA_002084"
     CDS             33046..33195
                     /gene="hokB"
                     /locus_tag="ACHJWA_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFO4308681.1"
                     /translation="MKRNPLVVCLLIICITILTFTLLTRQTLYELRFRDSDKEVAALM
                     ACTSR"
     gene            complement(33173..33241)
                     /gene="yncP"
                     /locus_tag="ACHJWA_002085"
     CDS             complement(33173..33241)
                     /gene="yncP"
                     /locus_tag="ACHJWA_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFO4308682.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(33267..33440)
                     /locus_tag="ACHJWA_002086"
     CDS             complement(33267..33440)
                     /locus_tag="ACHJWA_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4308683.1"
                     /translation="MKKLALILFMGALVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(33685..34215)
                     /gene="cybB"
                     /locus_tag="ACHJWA_002087"
     CDS             complement(33685..34215)
                     /gene="cybB"
                     /locus_tag="ACHJWA_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFO4308684.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            34404..35405
                     /gene="gap"
                     /locus_tag="ACHJWA_002088"
     CDS             34404..35405
                     /gene="gap"
                     /locus_tag="ACHJWA_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO4308685.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYKVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(35447..36886)
                     /gene="aldA"
                     /locus_tag="ACHJWA_002089"
     CDS             complement(35447..36886)
                     /gene="aldA"
                     /locus_tag="ACHJWA_002089"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFO4308686.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(37083..37883)
                     /gene="ydcF"
                     /locus_tag="ACHJWA_002090"
     CDS             complement(37083..37883)
                     /gene="ydcF"
                     /locus_tag="ACHJWA_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFO4308687.1"
                     /translation="MNITPFPTLSTATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDVPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            complement(37999..38376)
                     /locus_tag="ACHJWA_002091"
     CDS             complement(37999..38376)
                     /locus_tag="ACHJWA_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4308688.1"
                     /translation="MDERFINITPTSLSFKHADLSGLSYKNARLELALICPAIKSEVK
                     ISFDWIHSFRVTDEGDLLGMSGKQGINQTGIYRVANSTYLTWFTMQSCEIHKNKMIEH
                     YVIATSNDVIDVLSTVSPQIVYD"
     gene            complement(38501..38932)
                     /locus_tag="ACHJWA_002092"
     CDS             complement(38501..38932)
                     /locus_tag="ACHJWA_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4308689.1"
                     /translation="MNVRNVKFDNLSFYEEKIRWRMRRQISNAEAIFYENQEEHIRRF
                     LGRYTCRKPVLVYWESEQRWTSVNGLEVMSMFGEHCHHIYLDDIKKKIQTELPENKTL
                     DFKRQLQFIILGDDKIRIWVPEGKLYFALMNVLQMFPMKNG"
     gene            complement(38919..>39220)
                     /locus_tag="ACHJWA_002093"
     CDS             complement(38919..>39220)
                     /locus_tag="ACHJWA_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4308690.1"
                     /translation="LVETRMECHHTEYNFILTAELYVDEFTAGRERKRFEFTGVEHLS
                     QVMDIAELIDNQNAGNIKDATIVYKAKKYKISLHLFGGFLSFTFKTMRVIDNECA"
BASE COUNT         9836 a         9960 c         9331 g        10093 t
ORIGIN      
        1 ataatttcat tcctttttag tatttcttgt aacgttggta actgacatac tgcatcagca
       61 atcgcactga tattcgtggc attatcctga aaccattcat gcccaggccc ccatgtgcgc
      121 atagtgcaaa gataacagtc caccagcgtt agttgctcac caaacgcata aggttcagcg
      181 gtcaattgtg aattcagcca gacataaagc gatttacgat attcaataac attcttcttt
      241 aactgttcag gtgcgtcaga tgcccaacgc tcagggtaat cggcgaaagt gaacgttgga
      301 tagacattag caaccagcca gaccaataac cgttgaaact gctgacgttc ggcacgccca
      361 accggtggcg caaggtccgg gcgacgatcg agaaccatca atgcaatcgc cgctgtctcc
      421 gtcataatct cgtcattttc cagtgccagt gtcggaacct gacacaacgg gttcagggtt
      481 ttcaataact cgcgtgaagt tccctcgtgg tcaaaaccac tgacatcaac gaactgataa
      541 ggaatatcag ccagtgttag catcagctca ctgattgtag agccccatcc gggtacgcca
      601 tacaccttaa tcatatgccc ctcacaatct tttaaagatt aaatgtagga cagtacaaag
      661 gttaaccagc aaaaggattt gtcatttttt cgtcagttgt attaatcccc ttcaggattg
      721 taaggggatg ttttgcatcg tttatcgata acgggtagga tgctgcgccg atagttttat
      781 acccataaag aataatggtg ataactatca tcgccaggat gaataaacat tgttcatggc
      841 aacttatatg actttttcat taaagcaatc agggagagca atgagtaaac acgacaccga
      901 cacttcagat caacacgccg cgaaacgccg ctggcttaat gcccacgaag aggggtatca
      961 caaagcgatg ggcaatcgcc aggtacagat gatcgccatt ggcggcgcga ttggcaccgg
     1021 cttgttttta ggtgcaggag cccgactgca aatggcgggt ccagcactgg cactggttta
     1081 tttaatttgt ggcttgtttt cgttttttat tctgcgtgca ttgggtgagc tggtgctaca
     1141 ccgcccttcc agtggcagtt ttgtctctta tgcccgtgag tttttgggtg agaaagccgc
     1201 ttatgttgct ggctggatgt acttcatcaa ctgggcgatg acggggattg ttgatattac
     1261 cgccgtcgct ctgtatatgc attactgggg tgcgtttggc ggcgtgccgc agtgggtctt
     1321 tgcgctcgct gcacttacca tcgttggcac catgaatatg atcggtgtga aatggtttgc
     1381 ggagatggag ttctggtttg cgcttattaa agtgctcgcc attgtgacct ttttggtcgt
     1441 gggtacggtg ttcctcggta gtggtcagcc gctggatggc aacaccactg gctttcattt
     1501 aatcaccgat aatggcggct tcttccccca cggtttgctg cctgcgctgg tgttgattca
     1561 gggcgtagtg tttgcttttg cctccattga aatggtgggt acagctgccg gagaatgtaa
     1621 agatccgcag accatggtgc ctaaagcgat taacagtgtg atttggcgta ttggcctgtt
     1681 ttacgtcggc tccgtggtgt tgctggttat gttattgccg tggagcgcgt atcaggcggg
     1741 gcaaagtccg ttcgtgacgt ttttctctaa actgggtgtg ccatatatcg gcagcattat
     1801 gaacattgtg gtgctgaccg ctgccctctc cagcctgaat tcaggtctgt actgcaccgg
     1861 acgtattctg cgctcaatgg cgatgggcgg ttccgcaccg agttttatgg cgaaaatgag
     1921 tcgtcagcat gtgccgtatg ccgggattct ggcgacactg gttgtgtatg tcgtcggcgt
     1981 attcctcaac tatctggtgc cgtcgcgcgt atttgagatt gtgttgaact tcgcgtcgct
     2041 gggaatcatc gcttcatggg cgtttatcat cgtgtgccag atgcgcctgc gtaaagcgat
     2101 taaagaaggc aaagcagcgg atgtcagttt taaactgcct ggcgcgccct tcacttcctg
     2161 gctgacatta ctgtttttac tgagtgtcct tgtgctgatg gcgttcgatt acccgaacgg
     2221 gacttatact atcgcggcgc tgccgattat cggtattctg ctggttatag gctggtttgg
     2281 tgtgcgcaaa cgcgttgctg aaattcacag cactgcgcca gtcgtcgagg aagatgaaga
     2341 aaaacaggaa attgtgttta agcctgaaac ggcgagctaa ttgcaaaatt gcccggctac
     2401 gtgccgggca aatttagatt actgacgaag gtgtgccgca tgcggcgtga tcaaagtcag
     2461 gcaagtctct ccgttatcac cctcgccata tctattacag cgcaatacga atcacatcgt
     2521 ctggctgggt agcttcctgc tgtttagtgg atttttgttt cacactgaca tacagcgttt
     2581 tgccatcggc agacagcgcc aggctgttcg gatgagtcgg cgtatcgaac gttttcacca
     2641 ctttatagct tttcgcgtca atcacactga ctttacctgc ctgacgatgc gttacgtagg
     2701 cttcattacg cgctgggtta aacagcacag ccagtgattc cggtgccgca accttcgcca
     2761 gaatattgcc attacgggta tcgaccacta acacttctgc cgctttagaa tcggtgataa
     2821 atgcacgctg cctggcggta tcaaggctga tgttgataaa gaagtgctct ttgccgtcat
     2881 ccagcagctt tttacggctg aggattttat tgtcggcggt gtcgatggta atcaattcgc
     2941 cgtcagcgtt agtggtgtaa agacgtttgc ctttgctatc cagtgccaga ccggtactca
     3001 ttttaccggt gttctggatg gcggttttca gtttgatatt ctcgccatca acgacccaaa
     3061 tcacgctatc tttaccaata ccactgatgt aaacggtgtt cgtggcatca tcagctacca
     3121 gctcacgcgg ttgcagcggg cgcacctctt ccgtgcgctt acgatcatcc agcaccagac
     3181 ggcctttcac ctcgccagtt ttggcatcta tcgccgtgac cgcgctgttt acggtgttac
     3241 caaaccacaa cgtctgagtc gtgttattga tggtggcacc aaacggcttg agatcgttat
     3301 ggatcgcctg cgtcacttcc agagtgaccg gatcaagacg ataaaccacg ccgcctttat
     3361 ccagtttgcg gctttgcgaa gtggcgagcc acagcgcgtt ttcttgctgg ctataagcca
     3421 tttcgtaggc acctttacct accgctttac gcagcatttc ttctgcggcc tgcgtactga
     3481 atgatgaagc aacaagcaat gaacctaaca gtaatgaacc acgcaggcgc aatgaaaaca
     3541 gataacgtaa atgcatgacg actccctttg atgaaccgat atttttgctg cttcacaatg
     3601 cttcgctaag cgtaatcatc gttacgctct tgttataaat gggaatggta atcattattt
     3661 actcaaatgt ggagcatttc ttgccgttac gactacaatt ctctcaatta attaacgtat
     3721 cgtgccgttt cgttttccat aaatttacaa tttcattacc atgacggtgt tttcccattc
     3781 ttaacctggc ttttttcatg aagattgttt tcgttcgaca gaccgttttg cccgccctgc
     3841 ttgtcctttc ccccgttgtt tttgccgctg atgaacagac catgattgtc agtgccgcgc
     3901 cgcaggtggt ttcagaactg gatacccctg cagcagtaag cgtggtggat ggcgaggaga
     3961 tgcgcctggc aacaccgcgc attaacttgt ccgaatcact gaccggcgtg cctggtttgc
     4021 aggtacaaaa ccggcagaac tatgcgcaag atttacagct gtcgattcgt ggatttggct
     4081 cccgctccac ttacggaatt cgcggtattc gcctgtatgt ggacggtatt cccgctacca
     4141 tgcccgacgg gcaagggcaa acatccaaca tcgatttaag cagtgtgcaa aatgtggaag
     4201 tgctgcgtgg ccccttctct gccctgtatg gcaacgcgtc tggcggtgta atgaatgtca
     4261 ccacccagac cgggcaacag ccaccaacca ttgaagccag tagttactac ggcagttttg
     4321 gcagctggcg ctatgggctg aaagcaacgg gcgcaacggg agacggcaca cagcctggcg
     4381 atgttgatta caccgtctca accacgcgtt ttacgaccca cggctatcgt gaccatagtg
     4441 gcgcacagaa aaatttagct aatgccaaac tgggcgtacg cattgatgac gccagcaaat
     4501 taagcctgat tttcaatagc gtggatatca aagcagatga cccaggtggg ctaaccaaag
     4561 cagaatggaa agcgaatccg caacaagcgc ctcgtgcaga acagtacgac acgcgaaaaa
     4621 ccatcaagca aactcaggct gggttgcgct atgaacgtag cctgagttcg cgggatgata
     4681 tgagtgtgat gatgtatgcc ggagagcgag aaacgaccca gtaccagtca atacccatgg
     4741 caccacaact taacccgtca catgcgggca gcgtgattac cctgcaacgc cattaccagg
     4801 gaatagacag ccgctggaca caccgtggtg aactgggcgt tccggtcacg ttcactaccg
     4861 gcctgaacta cgaaaacatg agtgaaaacc gcaagggcta caataacttc cgcctgaata
     4921 gcggcatgcc ggagtacggg caaaaaggtg agttgcgtcg cgacgaacgc aatctgatgt
     4981 ggaacatcga tccctattta cagacgcagt ggcagctgag cgaaaaactg tcgctggatg
     5041 ctggcgtgcg ctacagctcc gtgtggtttg attccaacga ccattacgtt actccgggta
     5101 acggcgatga cagcggtgat gccagttatc acaaatggct accagccgga tcgttaaaat
     5161 atgcaatgac cgatgcctgg aatatctatc tggcagccgg gcgtggtttt gaaacgccga
     5221 cgattaatga gctgtcttat cgcgctgatg ggcaaagcgg tatgaacttt ggtttaaaac
     5281 catccaccaa cgatacaatt gagatcggca gtaaaacgcg tattggtgat gggctgctta
     5341 gtctcgcatt gttccagacc gacaccgatg atgaaattgt tgtcgatagc agtagcggtg
     5401 ggcgtacgac ttacaaaaat gctggaaaga cccgtcgtca aggcgctgaa ctggcatggg
     5461 atcaacgttt cgcgggagat tttcgcgtaa acgcgtcctg gacctggctt gatgcgacct
     5521 atcgcagcaa tgtttgcaac gaacaggatt gtaacggtaa tcggatgcca gggatcgccc
     5581 gtaatatggg ctttgcgtcg ataggttatg taccggaaga tggttggtat gcaggcatgg
     5641 aagcgcgtta tatgggcgat attatggcag atgatgaaaa tacggccaaa gcgccgtctt
     5701 atactctcgt cggcttattc accgggtata aatacaatta ccacaattta actgtggatt
     5761 tgtttggtcg tgtcgataat ttattcgata aagaatacgt tggttctgtc attgtcaatg
     5821 agtcaaacgg gcgatattac gaacctgcgc ccgggcgaaa ttatggtgtc ggcgtgaata
     5881 tcgcatggcg atttgagtaa ttaccttgat gcccggtatt tgccgggcat ttactttaac
     5941 gattgtattg ctggtataaa atagcaatat tttgttgcat aatttcagca agacaatgtc
     6001 tgcatccctc tttgtctttt gcttttaacg cagcgagtaa cagatgatag ttttctatat
     6061 gctcccgtaa ttccgctgga ttaatcgctt catagagata atgtaaacca ggtcccatcc
     6121 tgacccacag ttgctcaatc atctcacaca ggatgggcat atttgagcga tgataaatcg
     6181 ctaagcgaaa tagcctgttt acattaatga tttgttccat gtcgcccttt tcttgcgcct
     6241 gctgtaattt ctcgagcaat tcctggagtt ccgccaggtc ttgcggggtg aggttttcaa
     6301 cagccagagc aactgccatt aattccagtt cgtagcggat ccgattgatt tcgtctaatt
     6361 gacgtttccc cacttccgga actgtaaatg cttgcgctgg tgcgacagaa agcgcattca
     6421 ccgaaaccag acgtaataat gcttcacgca caggtgtaat actcatacct aattgctccg
     6481 ccagattttt agtaatcagg cgtgcgccag gttttagtgc gccaatacta agctgatgtt
     6541 tcaggtcgtt ctcaacctgc agagtcaaac tgacatgttt cattttttcc gttccaggca
     6601 ttttaattcc ttcgcgtgtt ttctgtccaa ccctgcctgc atggcagaag gaattcacct
     6661 ggtgtattaa agtgaataat cataaaccac tcacctttgt tgttattagc agcagaacaa
     6721 tgatgtggtt agttaattaa agctgaaaat aagctttttg cctggatttt tatggcggct
     6781 tacgccgccg ttgaaacttt aatcatcacc cgccacgcgg atcaccactt tgccgaagtt
     6841 tttacccttc agcaggccga taaacgtctg tggcgcattc tctaaaccgt cagtaatttc
     6901 ttcgcggtag tggattttat cctctttcac ccattgcccc atctccctct gaaactcatg
     6961 gatgcggtga ccataatcct gagcgataat aaaaccttgc aagcgaatac gttttttcag
     7021 cactgtagcc atcaacagag gtaaacgatc cggaccgggt ggtagctctg tagcgttata
     7081 gctgctcact aatccgcaga cgggaatgcg cgcagatgta ttaagtaacg gtagcaccgc
     7141 atcgaatacc ttaccgccca cgttttcata atagatatca ataccttttg ggcacgcttt
     7201 cgccagttgt tcggcaaaat catccgcgtg gtgatcaaga caaacatcga agcctaacac
     7261 ctcggtagca tggcggcatt tttccgcgcc accggctacc cccaccactc tgcaaccttt
     7321 aagtttgccg atttgcccca ccgtcgcccc cactggtcct gtcgccgcag ctaccaccaa
     7381 cgtttcgccc tctttaggct gaccgatatc cagtaggccc atataagcgg taaagcctgg
     7441 catccctagc acacccagcg accacgatgg attttgcgga tgatcgccaa gtttcaccag
     7501 atcatcacca ctggatatgt catagtcttg ccatccactg tagcccagca cccagtcgcc
     7561 agactgataa tcaggatgat tcgactccac gacacggctc accgtaccgc cgaccatcac
     7621 gccgccaata tcaaccggcg gtgaataaga tggctcatcg ctcatacgtc cacgcatata
     7681 cgggtccagg gacaaataaa ctgtgcgcag taacacctgt ccttcacccg gtgtggcgac
     7741 atcatcttct tcaagacgga aattctccgg aaccggtgcg ccatgtggac gcgaggccag
     7801 aacccaacgt cgattacgct gcttttgttg ccccataatg ctctcctgtg cttttggcat
     7861 gaatccatca atagtagcag gctcgcagga ttggttttgt ttgcagccgt caccccccct
     7921 ctgcaacacg caataatttc atcgaggaat ccgttgcttc gggctgtttt cgatagtatt
     7981 gataacatca tcatcattat tttattgagg ccagacatgt ccatccgttt tgcccgcaaa
     8041 gccgactgtg ctgccattgc ggaaatttat aaccacgccg tgttgtatac ggcggctatc
     8101 tggaatgacc aaacggtgga tgcagataac cgcattgcct ggtttgaagc gcggaccata
     8161 gcaggttatc cggtgctggt aagtgaagaa gatggcgtag tgacgggata tgcctcattt
     8221 ggcgactggc gtagtttcga tggttttcgc cataccgtgg aacattcggt ttatgtccat
     8281 cccgatcatc agggcaaagg tctggggcgt aaattgttaa gccgattgat tgatgaagcg
     8341 cgagattgcg ggaagcatgt catggtcgcc gggatcgaat cgcaaaatca ggcctcgctg
     8401 catctgcacc agtcgctggg atttgtcgtc accgcacaaa tgccgcaggt gggcactaaa
     8461 tttgggcgct ggctggatct gacatttatg cagttgcaac tcgacgaacg cactgaaccg
     8521 gacgcgattg gatgaatcag tcgctcaccc ttgccttttt gattgccgcc ggaattggtc
     8581 tggtagtcca gaactcgctg atggtgcgca tcacccagac ctcctctacc attctcatcg
     8641 ccatgttgct gaactcactg gtggggattg tgctctttgt ttctatttta tggtttaaac
     8701 aaggtatggc ggggtttggc gaactggtgt ccagcgtgcg ctggtggaca cttatacctg
     8761 gcttgctggg atcgtttttt gtcttcgcca gtatcagcgg atatcagaat gtgggagcgg
     8821 cgacaactat tgcagtactg gtcgccagcc agctgattgg cggtctggtt ctggatatct
     8881 tccgcagtca tggcgtacca ctacgggcgt tgttcggccc aatttgtggc gcaattttac
     8941 ttgtggttgg cgcctggttg gtagccagac gctcatttta atcacaggat ggtgccgcct
     9001 ttggttaact gttcatggcg cgcttccata tcttctttat gcttgcgacc gtgatgagca
     9061 atagcggtgc gcaaacgctg ctgctgggtg tagcgatctt cccgacttaa ctccgcgtca
     9121 tcgctcaggg cgattaataa ttcgttcata tgggcaatga cgctctcttc aatcgcggca
     9181 tccacgcgcg cgatgacttc atccagatgc gacatacttt ctccttagcc acttgtgaaa
     9241 tttatcgatt catccaaatg atataagtct gcgtggtgcc gtcaagccag tgagtaagat
     9301 ttaaaacagt gaaaacaaca gcgccaccgg gaaactcatc ggccatgttg cccccaccag
     9361 aaaggcgctt aagaaacgga tgcgtttctt atcgtgagaa agaaaccaag tgataagaaa
     9421 tgcgattgct gccataaccg cataaaaaac aagcatgtgt tgatagagag acatgtacgg
     9481 gcgtccagcc agatgaataa aacgcgcgca aatatgcgtc attttttgac gtgcatcaag
     9541 ttttttaacc ggtttgagaa aaaaacatca aacagcaact tgcccttatc catcttcaga
     9601 atgagtatta ttgcggattt tcttaacccg tactatacac ataggggcaa tgataaaagg
     9661 tggcaaaaat gaatgtttcc agtagaactg tagtactgat aaatgtcttt gctgctgttg
     9721 gtttgtttac tcttatctct atgagatttg gctggtttat ttgatgtagt gattttgcac
     9781 gcagcacgta ctgtcagaga aatcagtgct ctgacagtac gcaagatcat ctcatactct
     9841 tacgatctgc aatattatcc ttaatgttta accatgacgt ggcggacgac ggtgtaatcc
     9901 tccagcccat aaagtgacat atccttgccg taaccagaaa gtttctgccc accgtgcggc
     9961 atttcactta ccagcatgaa atgggtattg acccaggtac aaccatattg cagccgtgca
    10021 ctgacgcgat gcgccctgcc cacatctttc gtccataccg aagatgcaag tccgtactgg
    10081 ctgtcattcg cccagtttac cacctgttct tcgttgtcga agagcgtaac actcactact
    10141 ggaccaaata cctctttttg cacgatggca tcgtcctgta atgcgccagc cagcagcgtc
    10201 ggcgcatagt aatagccatt acccttgcgc ttttcaccgc cagtgatcac tttgatgtgc
    10261 cctgtcgctt tcgcctcttc tactgccttg ctgacgcgtt cgagatgcgc cagcgagctt
    10321 aaaggtccaa gctccgtaga ctcgtcatcc ggcgcaccag attttaacgt tgccactgca
    10381 gcaccgagtt tttccaccag cgtatcgtaa atgccttttt gcgcgtagat ccgacaagcc
    10441 acagtacaat cctgtccggc attgtaatag ccaaatgtac gtacaccttc gaccactgct
    10501 tcaatatccg catcatcaaa aacaatcact ggcgctttgc caccaagttc catatgagta
    10561 cgcttaatgg agggcgcggt atggctgatg atgtgctcgc cggtggcgat agagcccgtc
    10621 agcgacacca tccgcacttt gggatgaccg gtcagcggat cgcccaccgt tttgcctctg
    10681 ccaaacagta cgttaatcac gcctgccggg aagatatctt tcgccagctc tgccaacttc
    10741 aacgcggtca gcggggtaat ttctgatggt ttaagcacta cgcagttccc tgccgccagc
    10801 gccggagcca gtttccacgc tgccatcatc agcggataat tccacggtgc gatagaagcc
    10861 acgaccccca acggatcgcg acggatcatc gaagtatgac cttcaagata ttcacctgcc
    10921 gccagaccat tcagacagcg cgccgcaccc gcgaaaaagc gaaaaacatc gacaatcgcc
    10981 gggatttcat cattgaacgc actatgcagc ggtttgccac aattacggga ctccagttcg
    11041 gcaaaaacct gaccattttc ttcgataaca tcagccagtt tcagcagaca ttccgcacgc
    11101 actttcggcg tggtttgccc ccattcggca aatgctgcat ctgccgcgcg cacagcagca
    11161 tcgacctgct ctgcggatgc ctcggcaatc tccagtaaaa cgtcccccgt tgccggatta
    11221 tagacaggct gtttttcccc ttcgccgcta accagttctc cgttaatcag taacttatgt
    11281 tgcatagcat attcctgtat cagtggttat tgaccattgt cgccttcgcg ggttagccac
    11341 caggctccca ggatcggcaa ggttgttacc aacataacca gcagtgccac cacgttagtt
    11401 accggtacat cacgcggtcg cccaagctga ttgagcaacc acaacggtaa cgttcgttca
    11461 tgacctgccg taaaggtcgt aacgatgatt tcatcgaacg acaaggcaaa cgccagcatt
    11521 cctcctgcca gtaacgccga actgagattc ggcaacacta cgtagcggaa ggtttgccag
    11581 ccattggccc caagatccat tgacgcctca accagactcc atgaggtgcg gcgaaaacgg
    11641 gcgatgacat tgttaaacac cacaactaca caaaaagtcg catgaccgac cacgatggtg
    11701 aaaaatcccg gctccagatt gatggtttta aaggcggtta ataacgccag accagtgaca
    11761 atgcccggca gcgcaatggg cagcagcagt aacagcgaaa tggcgttttt gccgaaaaag
    11821 tctcgtcgcc acagcgcggc agctgccagc gtccctaaca ccagcgccat taatgtcgcc
    11881 agcgccgcca ctttaagtga cagtgtcacg gcatcaagaa tatcactacg ctgtgctgcc
    11941 acgctaaacc agcgcagcgt caggccctgc ggtggaaaac taaacgccgc atcttcagtg
    12001 ttaaacgcat aggcggcgat tatcaggatg ggaaaatgta ggaaaacaac gccgccccag
    12061 gccgccagtt tgaggaaaaa cggtgcgcgt tcagagtgca tcaaacgctc ccagacgttt
    12121 cacgaacgcc aggtacagtg cgataagaac aatcggcacc agggtgaatg ccgccgccat
    12181 cggcatattg ccaatcgccc cctgctggga ataaaccata ttgccgataa aatatcctgg
    12241 cgggccaacc agctgcggga cgataaaatc gcccagtgtg agtgagaagg taaagataga
    12301 gccagcggca atacccggga tcgccagcgg caacaccaca tagcgaaagg tttgtcgtgg
    12361 acgtgcgccg agatcagctg acgcctgcaa caatgacggc ggcaaacgct caagcgccgc
    12421 ctgcacgggc aggatcatga acggcagcca gatatagaga aacaccagaa agcgccccag
    12481 cccggaagtt gacagcgtat tgccgcccac cgcaggtaat gtaaggaacg ctgtcagcag
    12541 tggttccagc ccaagatgtt gtaaaaacca ctgagccacg ccatcttttg ccagcaataa
    12601 cgtccaggca taggctttaa caatgtagct cgcccacatc ggcaacatta ccgcaatata
    12661 aaaaaacgct ttcattttcc cgctggtata gcgcgccata taccacgcca ttggaaaagc
    12721 cagaatggcg ctggcgatag tgactgccac agccatagtc agcgtgcgga gaatgatgtc
    12781 gtaattcgcc ggattaaaca gcgcacggat gttcgccagc gtcagttccg gcgttaccga
    12841 catggtgaaa tcgtcaaagg tataaaagcc ctgccataac agcgttagca gcgagccgaa
    12901 atagacaatg ccaaaccaca ttagcgggcc aagcagcagc aaaaacagcc ccagccccgg
    12961 attccgccag aagaaaccgg acaccttacc cagacctgga cgtgaaggtg attgcaatac
    13021 gttcatcgcc attcacctct cctcaaccag cggcaccatc acatcacgcg accaggaaac
    13081 catcacctgt tgtccgggcg tgagcgtggc aggcagttct tcgcctgtca tattggcctg
    13141 actcaccagc agtttttcac cgccgttcaa tttcagttca aaacgagttg ccgcgccctg
    13201 atattgcacc gcctggatcg tgccattggc ctgcagttca ccaggggtgt tgaggcggat
    13261 atgttccggc cgcagggcga agcttcccgt catgccacaa agtttctctg ccatcagtcc
    13321 atcaaaaaca ttcgatgtac caacgaaccc ggcaacaaac ggcgtgcgcg ggcgcatata
    13381 gagatcgcgc ggggaatcga cctgctcaat gcgtccatta ttgaaaaccg ccacacgatc
    13441 ggacatcgat aacgcttcgc cctgatcgtg ggtaacgcag ataaaagtga taccgagaga
    13501 ctgttgcagt tttttcagct caagctgcat ctgctcacgc aatttgagat ccagtgcgcc
    13561 gagcggttca tccaacagca atacgcgcgg ttcattcacc aatgctctgg cgatagcaac
    13621 ccgctggcgc tgaccaccag aaagttgtga cggtttacgt tgctgtacaa accccaacgc
    13681 cactttctcc agcgcctctt gcgccattgc gtgccgctgc tttttattca cgcctttgac
    13741 catcagccca taggcgacat tgtcaagaat cgacatatgc ggaaatagcg cgtagtcctg
    13801 aaagacagta ttcacgtccc gctcccacgg tggcagattg ctggcgggtt taccaaagat
    13861 agagatagcc ccgccggaaa gctgttcgaa gccagcaatc aggcgcaggc aggtggtttt
    13921 gccggagccg gacggcccca gcatagagaa gaactcacca tcttttatcg caatactgac
    13981 gccatctact gcgcgcacgt caccgtacaa ccgcgagacg ttgtcaaact ccactgcgta
    14041 cgtcataaag caccccagcg aagttagcga ccgcccataa tggcaatgta atcctgcgtc
    14101 cagcgactgt agggaacaaa cttgccccct tctgctatag gcgttttcca gaaggctatt
    14161 ttatcgaaat agttaaaacc gtttgtttca caacctttct cgcctaataa cgaactggct
    14221 ttacaccctt ccggcacgac tggtaacgag ccaaaccagg ccgccacatc gccctgcact
    14281 tttggcgtta atgaccagtt catccatttg taggcgcaaa ccggatgttt cgcttcgcta
    14341 tgtagcatgg tggtgtcagc ccaaccggta acgccctcct tcgggaaaac ggtagcgaca
    14401 ggctgacctt cggctttcag ggcgttggtc tgatagggcc aggcactgga agcgaccaca
    14461 ccctcgtttt tgaaatcgct catttgcacg gtagtgtcat gccagtagcg atggatcaaa
    14521 ctatgttgat cacgcagcac tttcagcacc gcctggtact gttcttcggt gagttgatac
    14581 ggatcgctga tgcccaactg cggctgagtg gctttaacga acaacgcagc gtccgcaatg
    14641 tagatagggc catcataagc ctgaacgcgg cctttattgc tcttgccgtc ctgcagattt
    14701 tgctcaacaa aaaccacttg ccagctatcc ggcggcgtcg ggaaggtttt agtgttgtac
    14761 atcagcaggt tcggccccca ttggtaaggt gtgccgtaaa ctttgccgcc aacgttaaac
    14821 cagtcgcctt taaccacgcg cggatcaagc gttttccagt tgggaatcaa ggcggtatta
    14881 atcggctgca cgcgtttacc cataatcaaa cgcagcgagg catcgccgga tgccgtaacc
    14941 agatcgtaac cccctttggt catcagactg accatctcat cggaagtcgc ggcggtttta
    15001 acattcaccg cgcagcctgt ctctttttcg aattgcgtta cccagtcgta ttgtttatca
    15061 gtttgtccgc gttcgatata tcccggccag gcgataatat ccagtcgccc ttccggttta
    15121 tctaaattgg taggcggttc ggcggcgtga gcggtcatta ttgtcatgct gagcgcacac
    15181 aggctgctgc gggcaaatgt cttgctcata aggtcttact cctgtcgtaa ttaattgttc
    15241 agccgccgtg ccgtaaaaat atctccttgt ttaaccatag acggcggctt gcaggcacac
    15301 cttcgcccct gagaaaattt aatcaggttg tgagctaccg cgcattatgc cgtctgtaag
    15361 gttatcttct ggagtataga caaggagtta ggaaatatga ctgctattcg aaattcatat
    15421 tacccaatct aagaggaatt aaattaaacg ctattacagc cgttcttgaa taagtttgcc
    15481 taattgtttt accgcctgtt cttcacgttc tccccacagc cacgcggtat taaaacgaaa
    15541 aaaacgtgac cagttttcac cggtagaaaa cattttacct ggcgcaatac tgatatgatg
    15601 cgtcagtgcc gccaggctca attcgccggc atctaacggc tcggggagct ccagccagag
    15661 aaaataaccg ctgtcattat gatgaatttt cacttccgca ggcagataac gcagcagtgc
    15721 ctgccaggca cgttgtttac gttccgcaag ctggcgacgc aggcgacgaa gatgggcgtc
    15781 gtatcggcgc gtggaaaggt aatccaccag cgcaagctgc atcggtgagc tggtggaaag
    15841 cgtactcatc agctgcaagc gttgaatttt acgtgcatgt tttccggcgg cgacccaacc
    15901 aatacgaaaa ccaggcacca gacatttcga aaacgaagag caatgcaaaa cgccatcgtg
    15961 gcgatcccac gctttcgcag gcagcggttt ttcccgtcca aaataaagtt cgctgtaaac
    16021 gtcatcttca atcagcgtta cgttgtactg attgagcaac gccaccagtt gtgctttttt
    16081 ttgcggcgtt aaggtaaatc cgagtggatt ctggctatta gtcatcagcc agcacgcttt
    16141 caccggatac tcctgcaacg ccagttccag cgcctgaaga tcgatccctt ctttaacatc
    16201 cgtcgccacc gataacgcct tcagccgtag ccgctccagc gcctgcaacg caccgtagaa
    16261 acaaggattc tctactatca cccaatcgcc cggttcagtt accgcttgca aactgaggtt
    16321 taatgcctct aacgccccgg cagtgatgac aatttcatca ggagaaatgg tgatcccctg
    16381 taaggcatag cgacgggcaa tagcctgacg cagttctgcg tttcctggcg gcaagttttc
    16441 aatcacgctc atcgccgtgg cggttttgct tacctgcgcc agcgagcggt ttagttgttg
    16501 gagggggaaa agtcgcgggt cgggaaaggc cgaggcaaac ggaacgaccg acggatcgcg
    16561 actggcctgc aacatatcaa aaatataagt gttgatatcg actgcttcat ctcgagtgac
    16621 tggaatgact ggcactttcg gcatttttat tgcctgtggc gcaacgtaat aacccgactg
    16681 cggtcgtgcg ataatatatc cctgactttc gagcaactga taggcatggc tgacagtcat
    16741 aaagctcatg ccagaaagcg ccacctggtc acgcaacgaa ggtaaacgat cgccgggttg
    16801 ccagataccc gacgcaatct gctcgcgtaa ttgttctgca agctgctggt attttttcat
    16861 tatcgaatcg taattatgtg cagatgattc ggcagtctat atcagttgtt aaaaatgaca
    16921 acttttcgtt aactgtaatt aaaccatcac cagcgataac tctttgccaa gcgcctttgc
    16981 cgcgagctgg acggcgtcga tttttgtcgc atgatgcaag ttaaataggc gagtaatctc
    17041 ttgtttaggt ttgccaattc gcctggctaa ctcttgctga gtgatttctg actgtaaaaa
    17101 agcatttaac agcaatacct tagaggcgac gctcaaaggt acttcaataa agtgatcatg
    17161 actatttaat ggcgaaggta aagggataag ctcgttatct tcaaaataaa aatcaaatgc
    17221 ggtcagtaaa gcatcttttg ccgcttccat cgcttcagcg acagtttcgc cctgcgtcaa
    17281 cgcttcaggg atatccacaa aagaaaccat ataaccgcct tccggcgcgg gtgtaagagt
    17341 gacgggataa cgcataattt cgactgtctc tcgcatgtca ttaacctttt taattggcga
    17401 ttaactcaaa ccgagttgtt tcaggattgc tttacgcaat ggttctttaa tctcatcgca
    17461 ggggtgacgc ggcatgacac tgcgcctccc atgaaacctg agtttcaaat ggttgctgcc
    17521 attcgctaca tcgacgccct gagattcgag ccaacgtctg aactcgcttt gtttcacatc
    17581 gcctccctgc ggctgagtat atcaacaaag taaacattag tgtttacctc cgtgtgtaat
    17641 ttgctcgaaa atagcactaa atcgccgcaa cggcagaatg caatgcaaaa tcaggaagga
    17701 agattccaga atttctttgc gctaatttgc aaaactgaat aataacctca agaaacgatc
    17761 ggcatcatag cggcaaaagg taagaaaaag gtggcaggat gtttacgaag gcgttatcgg
    17821 ttgtcttatt aacgtgtgcg ctgttctcag gacaactcat ggcagggcac aaaggacatg
    17881 aatttgtgtg ggtaaagaat gtggatcatc agctgcgtca tgaagcggac agcgatgaat
    17941 tgcgtgctgt ggcggaagag tcggcggaag gtttgcgcga gcatttttac tggcaaaaat
    18001 cgcgcaaacc agaagcggga caacgttgag tcgtttgatg ccggatgcgg cgtaacgcct
    18061 tacccggcag gcaatatcat gcagattcaa taagttgtga gtaaccagaa aactggcgtt
    18121 ttaccctttt cgcttcggca atgttttcag cagctcatcc ggacttacgg atgccactac
    18181 gcttcccggt aacggcattt tcagtatgtg atttttgatt ttgccaatga cgtgcatctc
    18241 gcatgggcgg caatcaaact ttagcgttaa tacttcatcg ccgttaacca gttgcattgg
    18301 cgtcgccttc cagcttttga tattgccttt cgcctgtttc gggcacagat taaaggcaaa
    18361 gcgcagacaa tgcttggtga tcatcaccgg gacttcgccc ttctcttcat gtgcctcata
    18421 cgccgcgtca atcagctgca caccatagcg atgataaaat tcacgcgctt tctggttgta
    18481 aacgttcgcg aggaaactca gatgtgtttg cggataaacc ggcgcaggat cagcaaccgg
    18541 tttacggctg ccgcgctggt aactggcaag acgcgcagca tccagcatgt cggcagcttc
    18601 acggcggaac tggtttaaca gactgtttgg tacaaacagc gcccccggca aattaatttg
    18661 cacatcgcgg gcgtaataga gggtttgccc cagttttgcc agaccatcct tcagattgtt
    18721 cattgctttt tctgcgttat tggcttcgtc gaactgccca tccagcgtat gcgttatgct
    18781 gaccccctct tcactggtga gagtcagaat caattgttcc tgccaaccgc ccagttcaat
    18841 gtctaccgcc acccgacgtt cgctagaggt ttttgtcagt gcctgctgcc agttatgatc
    18901 aagattacgg tttagtgggt gatgcgggcg aattttgtgc aaatctgctg gcatttcatt
    18961 gggccagacg cggtactgat tttctccggt tttctcgacc gtattggcac gaaaaccgac
    19021 gacttcacgt ttaatcaaca cgttcaggcc atcgccattt gccagtggct cggtaacggc
    19081 aacatcgaga tgatctttcg ccactttcac tacttcacct accggcaggc cgataaattt
    19141 cggcgaatcg aacgcgccaa tatcgccttt acgggcattc acaaaataat ctgtgctacc
    19201 acggtggaaa gtcttttccg tcgatggaac aaagaaatgt tcagtacgac ctgatgaagc
    19261 gcgcgccaga tcgccacgtt cttcaataat ggcatcaagc atctggcgat aatgggcggt
    19321 gatattcttc acgtagctca tatctttgta acgcccttca atcttgaagg aacgtacgcc
    19381 agcatcaatc agcgcgccga ggttggcagt ctgatcgtta tctttcatcg acagcagatg
    19441 tttttcatag gaaaccaccc gcccctgatc gtctttcaat gtgtatggca aacggcacgc
    19501 ttgcgagcaa tcgccacggt tggcgctacg ccctgtttgc gcatgagaaa tgtagcactg
    19561 acccgaatag gccacgcaca gtgcgccatg aataaagaat tcaatggtcg cgtccgtggc
    19621 ctggtgaatc gcgcggatct gatcaagatt cagctctcgc gccagcacaa tctgcgtgaa
    19681 gccaacatca gagaggaact tcgctttttc tactgtacga atgtcgcact gcgtactggc
    19741 gtgcagttca atcggcggaa tatcaagttc cagaatcccc atatcctgaa caatcagcgc
    19801 atcgacaccg gtctggtaga ggtcagtaat cagccgttgc gcgggttcca gctcatcatc
    19861 atgcaaaatg gtgttaagcg tgacgaaaat ttttgcacca taacgatggg caaacggcac
    19921 cagctcggca atatctttca ggctattact ggcattatga cgggcaccaa aaccagggcc
    19981 gccgatataa acagcatcgg caccgtgcaa aatagcttcg cgggcaatgg cggcatcgcg
    20041 tgccgggctt aacagttcaa gtcgatgaga agatacggtc acattcttat ccagtagctg
    20101 aaaaatggcg gctattttag ccacaagcgc gacgttgcga aatagttttc ccgcttaact
    20161 gcgcgggtaa tggatgaggg aatgaaaatg tacggtttgc tccccaccat tacgatagat
    20221 atgcgtgacg tctgcagcaa atcgaacgcc ttccccacaa ttaagggtct gccattcacc
    20281 atcaacgcac agatcaagtt gtccatcaat gacgacgaca tgttcgatta cccccttctc
    20341 atggggcgtc gattcgctta ttgcaccggg tgccatctga atcgagaagt gttcaaagca
    20401 gagttgtgga tcataaggaa acagcgaaat gatcaccatc gcctgctgtt gtggatcata
    20461 aactgaaggc gtagcggact gtggcggaga aataaatgtc gaaaatggca cgttcaaccc
    20521 agtggcaatt ttccataatg tcgcgaccgt cgggctggac tcattacgct caatctgtcc
    20581 taacattgct ttagaaacgc ccgtcgcttc tgccagtcgc gaaagactcc agccgcgctg
    20641 ctgacgtagt tgtttaagtg tggtggataa aaagcgagcg agattttcca tgcgactcct
    20701 ccggcaaaac ggaagtttat cacttgtgcg ttataacgga caaatgctac ggtgcctgta
    20761 cgctataacg cacgaggtga ctatgcgtct gttttctgtt cctccaccca cgctactggc
    20821 ggggtttctg gcggtattaa ttggctacgc cagttcagcg gcaataatct ggcaagcagc
    20881 gattgtcgcc ggagccacca ctgcacaaat ctctggctgg atgacggcgc tggggctggc
    20941 aatgggcgtc agtacgctga ctctgacatt atggtatcgc gtacctgttc tcaccgcatg
    21001 gtcaacgcct ggcgcggctt tgttggtcac cggattgcag ggactaacac ttaacgaagc
    21061 catcggcgtt tttattgtca ccaacgcgtt aatagtcctc tgcggcataa cgggactctt
    21121 tgctcgtctg atgcgcatta ttccgcactc gcttgcggca gcaatgcttg ccgggatttt
    21181 attacgcttt ggtttacagg cgtttgccag tctggacggt caatttacgt tgtgtggaag
    21241 tatgttgctg gtatggctgg caaccaaggc cgttgcgccg cgctatgcgg taattgccgc
    21301 gatgattatt gggatcgtga tcgttatcgc gcaaggtgac gttgtcacaa ctgatgttgt
    21361 ctttaaaccc gttctcccca cttatattac ccctgatttt tcgtttgctc acagcctgag
    21421 cgttgcactc cccctttttc tggtgacgat ggcatcgcaa aacgcaccgg gtatcgcagc
    21481 aatgaaagca gccgggtatt cggctcctgt ttcgccgttg attgtgttta ctggattgct
    21541 ggcactggtt ttttcccctt tcggcgttta ttccgtcggt attgcggcaa tcaccgcggc
    21601 tatttgccaa agcccggaag cgcatccgga taaagatcaa cgttggctgg cagctgccgt
    21661 tgcaggcatt ttctatttgc tcgcaggtct gtttggtagt gccattaccg ggatgatggc
    21721 tgccctgccc gtaagttgga tccagatgct ggcaggtctg gcgctgttga gtaccattgg
    21781 cggcagtttg tatcaggcgc tgcataatga gcgtgagcgc gacgcggcgg tggtggcatt
    21841 tctggtaacg gcaagtggat tgacgctggt cgggattggt tctgcgtttt ggggattaat
    21901 tgccggaggc gtttgttacg tggtgttgaa tttaatcgct gacagaaacc gataataaaa
    21961 agcgccatct aacgatggcg cgtctggaac tactttttgt taacgtcaaa catggtggca
    22021 tcggtcgcca tgtcatcaat aacttgttta atattttcga aagccattgg cgtactttcg
    22081 ttattcaggt ctttaccttc gccctgacga acgactttga taaccggttt attagtcgct
    22141 gcatcaatca gctcaccttc aaaatacaag cgagtatcca tggtacggtg gcctgtagcc
    22201 atttgcgtcc ccgcaaccac taatgcaaca ggaaccactt catagaattg cagcccttct
    22261 ttgctggtat ctacaccggt aatggcccca cggaaaatca gactacgcgg ccccgcggtg
    22321 gtaaccagtg gtttacgctg cgctatcgct tctttcattt cggtgttggt atagttcaaa
    22381 attttatcca gaactttctg ccctacctgg gtcgaaggtt tcggtaccgg ataataagtg
    22441 attgggttcc agacgatgct gtcatatttg ctttgatcaa aactcgggtc tacccaacgt
    22501 aaaaccggtt tacctgtagc cgaggttgtt tcttttaaat cagagtaatt gtttaaaaaa
    22561 ccagaatatt tatctggctg ggtgatttta gaggcacaac cagacagagc caacaagccg
    22621 cataaagctg caactttcgc aaatgatgtg gtacgcatga atagtttcct gagatgatta
    22681 tattactttt taagttatag caaaaagcgc cgcttcgtgt tgtggcaaaa aaagtgttgg
    22741 tgaaaataaa tttttttagc cccttttcag acgacatagt caatgccgca aaaggggcta
    22801 agtcaatcat cagtagttaa atttcagtaa gttaagctgc taaaaaactt cgactactgt
    22861 gattaatttc acatcccttc gataaaaaat atgcttaccg aaaagaagga cagtaaacca
    22921 tctcagcgtg gggaattttc acgcgctttg ttcttaccgg gtcatttttt acgtgccagc
    22981 atcgtggcga aacgcagttt aatacgatta ccgttggcgt cggtgcggtg cagctcgccg
    23041 acgtcttcat tgtatttcac catctcccag ccttcgtaat atcgacgtaa ttctccctct
    23101 ttgaaggcaa acgggaagcc gacggtacat ggataatcag cggtatccat cgccgccaca
    23161 atcaggttgt aaccgccagg tttagtgcaa cgttgcatat tggcaatcag ccctgggatg
    23221 gttttagcct caaggaacat cagcaccaca gtcgaaagaa taaaatcgta ctctccatca
    23281 aatgtgaggt tattcagatc aacgacccgg gtgtgtaaat tatccagatt ttcaatggat
    23341 ttaatgcgct cgacgttggc gatactcatg gcatttttat cccatgcgtc aacatcataa
    23401 ccattggctg ccaggtaaag actgttacga ccattgccac agcccagatc cagcgtttta
    23461 cccggtttaa ccactttcac cgcttccagt acttcagagt gtgtgcgggt taattcatat
    23521 ttatcagtaa aataattttc gtcacgaatg atcattcttt gtcctctgct ttcattaaaa
    23581 cggcgcgctc ggttctgact aacaattttc cctgcatcag aagcgccaaa gtacggatga
    23641 gcagtattgc aataataaaa ttggtaaaga taaacagcgg caccgccagc gtatggaaaa
    23701 atccattatc gctgccactc cccagatgca aaccggtggt tgccagtgca gatacgccga
    23761 acgagaaact ccagaatgaa gcattaaatg gctgggagag ataccatggc atcagacgta
    23821 gcataaacag cagttgcagc agtccataac cgaaaagcat tttcgccagc gtgtcaccct
    23881 cgccgccgtt gacgctcagc caggcactac aggccaccag cgcaggagcg agctgaatgc
    23941 cgagtgatgt ccgcagtgcc gtgggtaatt ctcccgaact acgcagacgc tgcaagatca
    24001 ccggttcaag gcttagccat gagaaaacgc ctgcgcctaa aaacaccaga ccggcgtcgg
    24061 tgtagcccaa cgcaccacag gccattgcgc tgataaagtt gttggcaact gtcggcagat
    24121 acagtccagg cgtagtagct tcttcagggt gagatccgcg ccataatccc gcagtttgcc
    24181 aggcggcata agccaactga accacgacac caaaactgaa caggcacacc gccagtgggc
    24241 gaaaccacgg aacaaaacca atcgccacca gcatcgtcgt tgcaggaaac aaactcacaa
    24301 aactgctcag cactggatgg cgaacttccg ccagcacgct atgcggaaag cgtatgagtc
    24361 gggtaataaa tgcgctagtc aataatcccc agatgatcat cgccagaatc accagcccat
    24421 cccctaacca gtggctgacc tgccaaacct ggctggcata gcgccaggca aatcccatcc
    24481 ctatcgtccc caacacaata ccaaagtagc ctgccggcaa attgagcact ttatcgctct
    24541 gcatcttctt actcattttg tttaatttat aaagtatatt tgaaatgcat tttatggaat
    24601 tttctcgagc atagccagag ccgcagaatt tgctacggtt atagtaagtc acctgtggga
    24661 aaacgctatg cgcaaatatc gtctaagtga agaacagcga gcctttagtt atcaggaaga
    24721 tggcactaaa aaaaatgtgt tattacggca gattatcgcc attagcgatt ttaacgatgt
    24781 gatagcagga accgctggcg gctggatcga tcgcgaaacg gtgttagcac aggaaggaaa
    24841 ttgctggatt tacgaccaga atgctattgc gttcggcgga acggtgattt ccggtaatac
    24901 gcgcatcacc ggcaccagcg tactgtgggg agaggtttac gcaacggata atgtctggat
    24961 cgataatagt gaaatcaacc agggtgcata tataagtgac agtgtcacca ttcatgactc
    25021 actggtttgc gggcaatgta gaatatttgg tcacgcctta attgatcaac attctatgat
    25081 cgtcgccgca caaggcttaa cgcccgacca tcaactcctg ctccaaattt atgatcgcgc
    25141 aagggttagc gcctcgcgta ttgtccacca ggcacaaatt tatggcgatg ccgtcatccg
    25201 gtatgctttt attgaacatc gcgccgaagt ttttgatttt gccagtattg aaggtaatga
    25261 agagaacaac gtctggctat gcgattgcgc taaagtctac ggtcacgcgc aggtgaaagc
    25321 tggcatagaa gaagatgcca ttccaacgat tcattacagt tcgcaggtgg cggaatatgc
    25381 cattgtggaa ggtaattgtg tgttaaaaca tcatgtcttg attggcggaa atgccgtggt
    25441 acgtggcgga ccgattctgc tcgacgagca cgttgtaata caaggtgaga gccgtattac
    25501 aggtgcggtg ataattgaaa accatgtgga gttaaccgac cacgccgtcg ttgaagcctt
    25561 tgatggcgac acggttcatg tccgtggtcc aaaagtcatt aacggcgagg agcgcattac
    25621 gcgaacgccg ctggcagggt tattatgaat cgataatacg cgcgtataag ttcacatcat
    25681 cataggcatc attcaggaac tcagcctgtt tcaggcaacc ttcaaggata aaaccattgc
    25741 gcaaagcgac ctggttgctt tgcggattgt ccacccgaca tttgatcacg aagcgtctaa
    25801 gttcaccaga ctgagcgtaa tgatgaatca gtgcctgcag cgcctgagaa atgatcccct
    25861 gcccctgatg agattcgtcc agtcagtagc cgatttcagc ggttttattc agtggttcaa
    25921 tacgattaaa cgagataacg ccgataagtt catcttcttt gaaaatcatg aacattttgg
    25981 catagccgcg ttgatgcaac atcacattac cctgcaccgt ttttcgcgtg tcctcttcaa
    26041 tctgaacgaa ctgcggccag tttagcgact gctgtaacca ggttttattt ttacagatta
    26101 actgataaag aggagtgacg tgattttctg caacagcatg taattcaagt gattcgctta
    26161 cttttatcgt ttcagtcatc ttatctccag tcaatccatg cagttgttaa aaaagccgcc
    26221 tggctggcgg cttaggaaag tttacacctc tttgacgctc tcatgctgca ggattttgag
    26281 catctcatct tttaactgta gcttttgttt tttcatgcgg accacttccg cattgtaccc
    26341 tcgatcgtcg gaaccttcct ttctggcaat ttcatgatca agtttattgt gtttatcgaa
    26401 caaggacata aagcgaggat tttcgttttt cagacgggat attaaatctc gatattctgg
    26461 aaacatacgc actccctgtt agtggctgac aaggtgtatc tgaaatacac accatcagat
    26521 tactcattta acttagggaa aaatgcgagc gggatcgtgt ttaaccagac ggcacctccc
    26581 gaagaaggtg ccgaagaaaa aacgattaac tcatcacgcg gtcgtcaaca tactgacgtt
    26641 tatccggcgc tggcgggaaa tactgataca gccatgtttc actgatagcg tcgccctgac
    26701 aacgcagata catccgcata tcgaccggat cagtggagtc cgaagtcgga taccagtcaa
    26761 actgaatacg ataaccatca atgggttcaa tatagagaat ttcgatttgc ttcgcttccc
    26821 cactggaaag cgtaatcacc ggctcaatgc cttttggtgc ggcagctttc agatcaccac
    26881 caacgaaatc gacggcaaaa cgacgcgccc atttttcggg atagtgttca cctggtgccc
    26941 aaccttccgg gaaaccgccc atgccggtac gcgtcgccat aacgcgcgct aatgggcaat
    27001 gaacaggcgg ttgcgcactc cagtacagac gatactggaa tgcaaactca tcacccgcct
    27061 ttacagcttt ttctggctgc cagaagcaga caatgttatc cagcgtttcg cccgttgttg
    27121 ggatttccat caggccgatg gtgcccttac cccacttgtt acgcggttcc acccacagac
    27181 ttgggcgttt gttataccag cccataatgt cctgataatg ggagaaatca cgatccagtt
    27241 gcaataaacc aaaccctttc gggttgttgt cggtgtaagc attgaactgc aatttttgcg
    27301 gattattcag cggacggcaa atccactcgc cgttgccccg ccacatggac agacgatcag
    27361 agtcatgaat ttgcggatga atggtgtcgc acatccgacg ttcattagta ccgcagctga
    27421 acatactggt catcggcgca atgcccagct gtttaatgtc tttgcgcgca tacaggtgat
    27481 tttccacatc cataatcacc tgacttttct cacaatggat agtgaactta taggcaccag
    27541 taatgctggc gctatcgagc aacgcataaa cggtaaaggt agttgccccc ggttttaccg
    27601 tatcaaacca gaaggcggta aagtcgggga actcttcttt actgtcggtg taagtgtcaa
    27661 tcgccaggcc gcgggccgac aaaccgtatt gatatgtgtc atcaacggcg cggaaataac
    27721 tcgctccgag gaatgatact acatcacggc gcgccagttc gggggcttta aacacgcgaa
    27781 aaccggcaaa gccgagatcg ctttgccctt ctaattgttt ggtatcaaca cctgcatcgt
    27841 tgtatttgaa caactccggg cgaaagtgaa tttcacgcgc cagatgtgtt gctggatcta
    27901 cagaaaacat acgaacgcgg cgacggaatc ccattcccat atggaagaac tgagcgtcca
    27961 gttgacggtt ctcaacgtta tgccagagcg atttttcggc gtcgtattga atactgttat
    28021 aagcctgcgg cgtcattgtc gccagcgtgt caggtaacgg acgaggcgca ccacgccacg
    28081 ctgtttgcgc taagtcgtgc gccattgact gtagaatgga gaagtcaaaa cgctgggttt
    28141 gcccgtcggc aatatcagaa tctgccgcga atgccgcctg agaaaaaaga gaagcaatgc
    28201 cgctggtacc gcacacggcg gccatagcca ttgaaccttt aataaatcgt ctacgatcca
    28261 tacctgaaag tgagtccttc tggtgttgtg aatggtcccg cgctggtcgt tttcagcggc
    28321 aagaaagtgc ttatttatga ccgctcactc tagacaatat ttattaataa tccaattgtt
    28381 gaactgagag aatatgcata aatcggcaat ttttttatgt cgatgggatg agcctgattc
    28441 gtttgtaaaa tcaggcaaat ttttgcaaag gcggatggtc agaatttaaa gcaaaccaca
    28501 gcgacgttta ctgcgttctt ccgcttcctg ataaagctgg aactcatcca aaaccggaca
    28561 acccaacgcc tgctcaaatg cttcgcgact ggcattaccg tggctgcgtg cctgcgtttc
    28621 attacccaga tttgactgga agatccccgc cgcgctaacg ggcaagaaat cttcataggt
    28681 aatgggttgc gccgcgaccc aaccacgttc aattaagggc tgtggatcgt ctccgggatg
    28741 aatcgcctga cgatgcgcct cacctgaagg cgtcagacgg taccggaacc atgccagccc
    28801 ttgctgacgc attaaaaact cactgtcagg aaaagtgcgg aaggtttcct gtaaatgcat
    28861 ttggtgagtg agattatcct gcccggttcc agcgttacgc agaagatcat catacagttg
    28921 tcgacctttc ggcgttaatg ccacgccacg ctgctcaatt tcaccaaagc gcgcagtatg
    28981 cgtgccctgt ttctgccccg caaacaacac cgtctcttcc agtgctttaa agctggtctg
    29041 gcgtagtaaa atcggtacct cgcggcgcgg cggcccttcg atcagaattt ttggttcaat
    29101 tccgcattca ggcatcatcg actgcacccg gtcaatatcc agcgtacgtg gcgtcagatg
    29161 gttgatatgg catccaggaa aacagaccac atcagcaatc aaccgatgtt cgttgtgcaa
    29221 ggcgcgatag gtttcttcat ctaccgttgc tgactggtgc caacggaacg tttccagggc
    29281 ctcctgcaca aactcctgtg cctgtgtttc gttaaaaccg ccccgctggt catactcctc
    29341 taacagttgt cgacaacgtg gggtgaagat atcgcgctga cgcagaatct ccgccgcttt
    29401 ctggcgcaaa atttcgttct cgataagctc aaggcggagt aaggaggtaa aaacgcggaa
    29461 gggattacgc gccagagaag catcatcaat gggccgaaat gctgtcgaat gtaccggcac
    29521 ccctgcctgc gagagatcgt aatagctcac cgggtacatc cccataatgg caaacatccg
    29581 ccgaagagta gcaagctctt gtgcagtccc aacgcgaatc gccccatgac gttcaacatt
    29641 cagtcgcgcc agctcgtctg catttaccat tttttcgtgc agttgaggat tgttttccag
    29701 cacagccaga ttcacatcag ctaccagttc cagcagcgtg ccatattgcg gaacttcttg
    29761 ctggtacatg gctgacattg cctgcgaaaa ctgttcccga atctcatccg ccgtgatgct
    29821 gttcgccata gtgtctgcct ccagtggtca gtaatctgga gtgtaggtaa ccgcattcac
    29881 tctggcggga agaatttaca aactgtgatc tcgccgcgaa aacatcaata ttatccattt
    29941 tgctgtaaca taattgcttt aattgttaat aatattttgc aatcaagtta tcataatcaa
    30001 acaacttcac ttgtcagcga caccgcttcg tttttaacat cgcttatgga aaaaaatagt
    30061 ctgtttagtc agcgcatccg tttgcgccac cttcatacat tcgtagctgt cgcacaacaa
    30121 ggaactttgg ggcgcgcggc tgaaaccctt aatttgagtc aacctgcgct ctctaagaca
    30181 ttaaatgaac tggagcagct gacgggcgct cgcttgtttg agcgtggtcg tcagggggcg
    30241 caacttacct tacccggcga acaattttta acgcatgcag tcagagtact tgacgccatc
    30301 aacactgccg gacagtcgct tcatcgtaaa gaaggtccta ataatgatgt cgtcagggtt
    30361 ggtgcactac ctactgcggc actggggata ttaccttcgg ttataggtca gtttcatcag
    30421 caacaaaaag agacgacctt gcaagttgcg acaatgagta accctatgat tctggcgggt
    30481 ttgaaaaccg gggaaatcga tatcggcatt ggtcggatgt cagatcctga actgatgacc
    30541 gggcttaatt acgaactgct gtttcttgaa tcgctgaagc tggttgtccg ccctaatcat
    30601 ccgctacttc aggagaacgt aacgctaagc cgggtgctgg aatggccggt cgttgtatca
    30661 ccagaaggca ctgcgccacg ccagcattca gatgcattag tacagagcca gggatgtaaa
    30721 attccttcgg gttgtatcga aacgctgtct gcttcgctat ctcgtcaact tacggttgaa
    30781 tacgactacg tgtggtttgt cccttctggc gcggtaaaag acgacctgcg tcatgccacg
    30841 ctggtggccc tgcctgttcc gggacatggt gcaggcgaac cgattggaat actgacccgc
    30901 gtagatgcga cgttctcttc tggttgccag ttgatgatta acgctattcg aaaatcaatg
    30961 ccgttctgaa aggtgaaggg atctgtcgat ccctccttta acattttcac accggagcga
    31021 aactaactgg ttcacccgct ccgcgaggtt ctgccaacac agattgtttg ttcagacgga
    31081 atacgtccac cgcctcagtt aatcgtgccg cctgttcttc aagggacacc gccgccgctg
    31141 aggcctcttc taccagagaa gcattctgtt gcgtcacctt atccatttca gaaatcgcct
    31201 ggctaacctg cgttatgccc ctactttgct catccgaggc ggcggcaatt tcctgcatga
    31261 tatgtgtgac actcgcgacg gcatcaacaa tagtgctcat ggttttcccg gccgttgcca
    31321 cctcatccga tcccaggtca attaacctga ctgattcact gatcaagcct tcaatttctt
    31381 ttgccgcctg ggcgctgcgg cttgcgagtg tccgtacttc gctggcgaca acggcaaatc
    31441 cacgcccttg ctcacccgct cgcgcagctt caacggcagc attcagtgcc agaatattcg
    31501 tctggaaagc aatactgttg atgacggcgg tgatctcaga aattttcttc gaactcgtgg
    31561 agatagcgcc catcgttttt actacaccgg aaaccgtctg cccgccatcg ctggctttaa
    31621 tagaagcctc ttgcgccagt ttgctggcat aatgcgcgtt atccgcattc tgtttcaccg
    31681 tcgcagtgag ttgctccatg ctggcggcag tttgttcgat agccgccgct tgttcttcgg
    31741 tgcgagatga caggtccgca ttgccagctg aaatttcgct ggtgccacga taaatctctt
    31801 ccgcaccctg tcgaacagtc cctacggtca tccccagtga atgctgcatt tgctgtaaat
    31861 gacgacttaa gcgaccgatt tcattacgac ccgccggttc atccttcatc gtcagatcgc
    31921 cactagcgat tttttcaatc cgttgtgcgg catgttgcag tggacgaatg acgatccgac
    31981 gtagcaccat aaatgttatc agcgtcatga ccagggcaag cacaaacgca ccaatcatga
    32041 acatcccgcc caggcgggtg cgctgatggg ccagttccgc cagttggttg gctctggtgc
    32101 tacgtatctt aacggctttg ttcaaaatat cggtataagc attatccagc gttcggatct
    32161 gctcactttc atgattgata atcgcttcaa acatgccatt tttggcatat ttcaacatag
    32221 gttgcatacc cgtgatataa gcctgaaagc gttgatttaa ttcagtgtcg agggcttcat
    32281 cagcaggtgt tttcaccggc cgattctgat aagcacgata accttgctgc gactgtttaa
    32341 tctccgattc ggcttgcgca atatttcgct tcattgcttc catttccgca atacgactcg
    32401 cagccccggc ctgaatcata ttgatccggg ctgaacgcaa aaaatctgaa ctgttcgcta
    32461 acccggtccg aatctcaatc tccgcagtaa catcacgctg atcgcgatcg gcctgccaga
    32521 ggaaatagcc agccagggct gaacttaatg cgaatagaac taagataccg cctagaatgc
    32581 aggcaaataa cggaaccaac ctgatgtgat gcaaaaaacc taatcgctgt gagggagttg
    32641 tattcattac cgttgtctct cgtccaggtt tactcctgat agaaagtcat cggcatttga
    32701 cggtaattac ttacgactaa aagcctcttt gcgtcacatt tttacaacaa ttattgcaga
    32761 gaagattaat gccattggaa tgaagagagt aacgaatggt taaactggct aacccgaatg
    32821 acgaaaaatt ctggaaaaca gcacgcaatt tcgtcaggtg attattttcg tccctaagga
    32881 aaccacatag gatgctcacg ggtgcctgag actttctgtt ttcaggctaa ccgggaatca
    32941 gaaagacgaa ggccccgacc gatatttcta tcaaccgagg ccaacgaatg aaccaagcaa
    33001 ttcagatgtt agcctcttac ccgccatccg gcaaggagaa aggctatgaa gcgcaaccct
    33061 ctggtggtgt gtctgctcat tatctgcatt acgattctga cattcacact cctgacccga
    33121 caaacgctct acgaactgcg gttccgggac agtgataagg aggttgctgc gctcatggcc
    33181 tgcacgtcca ggtaagggca agcgcgggga ttttccccgc gcatctgact agcagattca
    33241 ttttctcatg gcaccccttt tacacttcag ttagtgcatg tttttttcga tgcactaata
    33301 gaaccatcat tacagacaaa tttgccgcct gccgtacagt gtgagatccc ccctttcgaa
    33361 ccagaacagg gtttgcgccc ggcatcggca taaaaggaaa caagcgctcc cataaacaaa
    33421 ataagtgcca gttttttcat ataacctcca tggtttatcg aatcttatgt tcgatttgaa
    33481 cgttatacgc tggtttattt tgataccgtt gatgagatca aagatcgggt gaattgatta
    33541 aaaataagcc agatggacgt atgggcaagg attatggttt tatttgtcat acaaataaat
    33601 ataataggcg cttccgatgt agacccgtat tcttcgcctg taccacgggt cggttttagt
    33661 acaggcgttt tctttaaata tccttcagga acgtttacgc ggcatcatgc gtagaagtgt
    33721 gttgtccttc cagaaatagt ggtgtgccag tgcagccgca gcgtgcaaac cgatgacaaa
    33781 atatcccaga ttcgccagcg tttcatgcca cgactttaag ctatctaccc gttcgaaatt
    33841 ggcctctgaa gcgtaaggca tcgtcaaacc aaacgcaaac cacgggttgc ctcggttata
    33901 catcatcacc aaaccaatca ctggcagcgc aataaacagg agataaatca ccaaatgtcc
    33961 caaatgcgcc agtcccgtca tcatcggttt tggcttaggt ataatcggcg gggttgggta
    34021 tttcagcctt aacaaaagac gaacgaccat cagcacgaga attgatatgc cgcaggaaac
    34081 atgaatcatg ttgatgagtg gccgatcgct acgtgggaag aaaccccgaa actccattgc
    34141 gcaatacgct gcgataacca gcaaaaagac cagccagtga atgctgattt gtaaccttga
    34201 gtatttattt tccataacat ttcctgcttt aacataattt tccgttaaca taacgggctt
    34261 ttctcaaaat ttcattaaat attgttcacc ctttttcagt acgttacgtt tcactgtaag
    34321 aatggagatt gcccatcccg ccatcctggt ctaagcctgg aaaggatcaa ttttcatccg
    34381 aacgttcctg acaggagaaa accatgagta aagttggtat taacggtttt ggtcgtatcg
    34441 gtcgactggt gttgcgtcga ttacttgaag tcaaaagcaa catagacgtt gtcgctatta
    34501 atgatcttac ttccccaaaa attctcgcct atctgctgaa acatgattca aactatggtc
    34561 cgttcccctg gagcgttgat tttacggaag attcacttat cgttgatggg aaaagtatcg
    34621 cggtttacgc cgaaaaagag gctaaaaata ttccgtggaa agcgaaaggt gcagaaatca
    34681 ttgtcgaatg tactggcttt tatacctccg ccgagaaatc gcaggcgcat cttgatgctg
    34741 gcgcgaagaa ggtgttgatt tccgcccctg caggtgaaat gaaaactatc gtttataaag
    34801 tcaatgacga cactctggat ggcaacgaca ccattgtttc cgtggcatca tgcactacta
    34861 actgtcttgc gccgatggcc aaagccttgc atgacagttt cgggatagaa gtcggcacga
    34921 tgacgaccat tcatgcctat actggcaccc agtcactggt ggatggcccg cgtggtaaag
    34981 atttacgtgc ttcacgcgcc gcggcagaaa atatcattcc ccacactacg ggcgcggcaa
    35041 aagccattgg tctggtgatc ccggaactga gcggcaaact gaaaggtcat gcgcaacgcg
    35101 tgccggtgaa aacaggttcg gtcactgaac tggtatcgat tctcggaaaa aaagtgactg
    35161 ccgaagaggt gaataacgca cttaaacaag caaccaccaa taacgagtca tttggttata
    35221 ccgatgaaga aatagtctct tccgatatca ttggcagcca tttcggttcg gtgtttgatg
    35281 ccacgcaaac ggaaattacc gccgtgggcg atttacaact ggtgaaaacg gtcgcctggt
    35341 acgacaacga atatggcttc gtcacgcagc ttattcgcac cctcgaaaaa ttcgctaaac
    35401 tctgacgcgc acaggcggag gaaaaaacct ccgcctcttt cactcattaa gactgtaaat
    35461 aaaccacctg ggtctgcaga tattcatgca ggccatgttt accatctgcg ccgccaatac
    35521 cggatttacg ccatccggcg tggaagcctt gcatagcttc gaagttttca cggttgatgt
    35581 aagtttcacc aaacttcagc cctttaatgg ctttcatcgc gacgttcaga ttttgggtat
    35641 agattgatga ggtcaggccg taatcactgt cattagccat tgagatagca tcttccagcg
    35701 tgtcaaatgc gactaccggc agaaccgggc caaaggtttc ctcatgcata atcgacattt
    35761 cctggcgaac atccagcagc aatgtcggcg gataataata tcctttcccc tctaccgctt
    35821 tgccacccaa cgccactctc gccccttctt ctactgcgcg cgccactttt tgctcgaccc
    35881 tttccagcgc cgcggcgtta atcaacggcc ccatcgcaat gtcgttgcgt tcagcggggt
    35941 taccaaattg aaccgcctgc atcgcttcac ccagccgatt gacgaactga tcataaatgc
    36001 ctttctgtac ataaacacgt tctgcacagt tacacacttg cccactatta atgacgcgtg
    36061 aatcaacgat ggctttgact gccagttcaa gatcggcatc gtccattacg atagctggtg
    36121 ctttaccccc cagttccagg cacactttgg tgatgttttt cgccgcagtc gccatgatct
    36181 tctcacctgc agagacgctg cctgtcatac tgaccattgc gacctttggg ttacccgcca
    36241 gttcttgccc aacggtttca ccacgcccca gaacaaggtt aaacacgccg cgcggaaggc
    36301 ctatttcatc gacgattttg gcgaatgcaa tcgcattgtt tggcgtaaat tcactgggtt
    36361 taatgacaat ggtattacca gtcaaaagag cgggagccat tttgcgggca atgaggaaga
    36421 acgggaagtt ccacggcaga atgccggtag tcacaccaag cgcacgttta aacagaagaa
    36481 tattttctcc tggacgatcg ctttgaataa tctcgccctc gtaacgccgt gcccactccg
    36541 ccatgtaatc gatatagtcg gcagtaaaag ccacttcgac ttcagccagc tgctggatct
    36601 tgcccccttc ttcaacaatc agcgcactga tttcactggc gcgttcgcgg atcccggcgg
    36661 agattttgcg caaccaactg gcgcgttcaa ttgcaggcaa cgcttcccat tctggttgtg
    36721 cacgttctgc tgcatcgatt gccttacggg catcctcggc ctgaccatcg ggaatgcggg
    36781 aaatgacagc ctctgtagca gggtttacca catcaatcca tgcgtctcca cgccaggtaa
    36841 caaactgtcc atcaatatac ataggatgtt gaacgggtac tgacatgggc gactcctgtg
    36901 atttatatgt ttgtttttgg taaatacatt gttaattaac atcaatcatc atagagacgg
    36961 catcatgaaa ggacaattct gtgaagggct tcataaaaac aagtgtaaaa ggaataagag
    37021 gcagaaataa aaggcaggag ttatgcccgt ttgcaaacaa gtggttcaca aacgggcaat
    37081 ttttaacgta acgagcgact ttccatcgcc tcgatgagca ccgcatcgtg tttcagcgtt
    37141 tgccatgcat ggatgacttc tgccggaaaa tcaacgtgaa cgataaaatc tcgcccgcgg
    37201 ggaccgtagc catcgctatc atcgcgtaaa cgcggcagct cgccagtgag tagtgagaga
    37261 taacgctcaa ccggccataa tccttgtaac tgattaataa agattacact gtctgcgtta
    37321 tttcctaact gaggaacgaa tccgggataa cttaaccagc gtggtacatc gggattgtcc
    37381 ccggtcatac ggcggaacgt cgccatcgtg cgccgctgca tggtggggtc ctgtacaacg
    37441 atagctgtgt gaactcgttc tacggcctga ttcaatagcg cgatgctaaa gcgtgcgttt
    37501 tcaccgcagt ttgttgactg gtcttcaatc cagatttttt catgcggaat atgccagaac
    37561 tgatgagcga tatccgccag gatggtcgct tctgctctgc cagtggtgcg gatagtgttg
    37621 tagtgcggat gctgtgcgat ggcgctatac aaaaaagtgg tcgagtgacc gataccacca
    37681 ctaatcagta aaggaatttg ctgatcgcga gcaatcttac atgccgcatc gatagtcggc
    37741 ataaccgcat ttcctgcaag tatgacgcaa tcagcctgat acggcacctc accggagaaa
    37801 tcatcctgcg ccagccactg tccgataaca tttatggcat ctatagttgc cgtcgaaagc
    37861 gtcggaaacg gggtaatgtt catggttcct ccttatcgtt cccttaagaa tactgcgtga
    37921 gataatagtg agaagcggag cactctgaaa ggaagataat gctttttcta tctcgccatc
    37981 gcgaaccaga cactggtttt agtcatacac tatttgcgga gaaactgttg ataaaacatc
    38041 aataacatca tttgacgtgg caataacata atgttcgatc attttatttt tatgaatttc
    38101 acaactttgc atagtaaacc atgtaagata agtggaattg gctactctat agatccccgt
    38161 ttggttaatt ccctgctttc ctgacatacc taacaaatca ccttcgtcag taacacgaaa
    38221 tgagtgtatc cagtcaaatg atatcttaac ttctgacttg atcgccgggc atatcagggc
    38281 cagttcaagc ctggcattct tatagcttaa acctgacaga tcggcgtgtt taaaagacaa
    38341 cgaagtcggc gtgatattta taaaacgttc atccatgtta acacccttaa tatatgagac
    38401 tgtactcaat ttaaccttct acaaaaatgg tgtgagtctg tttaagtacc atgtataatc
    38461 gataaccgtt gctgaatttt aaaattatct tgtccagtta ttaaccattt ttcatgggaa
    38521 acatctgcaa tacattcatt aatgcgaaat acaatttccc ctcaggcacc catattctaa
    38581 tcttatcatc ccctaatatt ataaattgca attgacgttt gaaatctaaa gttttatttt
    38641 caggtaattc agtttgtatt tttttcttga tatcatcaag gtagatgtga tgacaatgtt
    38701 caccaaacat actcatcact tccagaccgt ttacagatgt ccatctttgt tcactttccc
    38761 aataaacaag aaccggtttg cgacaggtat atctgcctaa aaagcgccga atgtgttctt
    38821 cctgattctc gtaaaatatt gcctctgcat tcgagatttg ccgacgcatt cgccagcgga
    38881 ttttttcttc ataaaaagac aagttgtcga atttgacatt acgcacattc attgtcaata
    38941 actcgcatgg ttttaaatgt aaaacttaaa aaaccaccaa acaaatgaag tgaaatttta
    39001 tacttctttg ccttataaac aattgtcgca tctttaatat tacctgcatt ttggttatct
    39061 ataagttcag caatatccat aacctgcgat aaatgttcca ctcctgtaaa ctcgaacctt
    39121 tttcgttccc ttccggctgt aaattcatca acataaagct ccgccgtcaa tataaaatta
    39181 tactcggtgt ggtgacattc cattctggtt tctactaaaa
//
LOCUS       DAZFAB010000035        38029 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZFAB010000035 DAZFAB010000000
VERSION     DAZFAB010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38029
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.035"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            120..731
                     /gene="ruvA"
                     /locus_tag="ACHJWA_002094"
     CDS             120..731
                     /gene="ruvA"
                     /locus_tag="ACHJWA_002094"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFO4308691.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            740..1750
                     /gene="ruvB"
                     /locus_tag="ACHJWA_002095"
     CDS             740..1750
                     /gene="ruvB"
                     /locus_tag="ACHJWA_002095"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFO4308692.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(1996..2781)
                     /gene="znuB"
                     /locus_tag="ACHJWA_002096"
     CDS             complement(1996..2781)
                     /gene="znuB"
                     /locus_tag="ACHJWA_002096"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFO4308693.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTDGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(2778..3533)
                     /gene="znuC"
                     /locus_tag="ACHJWA_002097"
     CDS             complement(2778..3533)
                     /gene="znuC"
                     /locus_tag="ACHJWA_002097"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFO4308694.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            3612..4544
                     /gene="znuA"
                     /locus_tag="ACHJWA_002098"
     CDS             3612..4544
                     /gene="znuA"
                     /locus_tag="ACHJWA_002098"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFO4308695.1"
                     /translation="MLHKKTLLFAALFAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSINGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4560..5882
                     /gene="mepM"
                     /locus_tag="ACHJWA_002099"
     CDS             4560..5882
                     /gene="mepM"
                     /locus_tag="ACHJWA_002099"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFO4308696.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            6002..6973
                     /gene="lpxM"
                     /locus_tag="ACHJWA_002100"
                     /gene_synonym="msbB"
     CDS             6002..6973
                     /gene="lpxM"
                     /locus_tag="ACHJWA_002100"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310592.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFO4308697.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRIAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(7104..8546)
                     /gene="pyk"
                     /locus_tag="ACHJWA_002101"
     CDS             complement(7104..8546)
                     /gene="pyk"
                     /locus_tag="ACHJWA_002101"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFO4308698.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGINYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(8674..9543)
                     /gene="hexR"
                     /locus_tag="ACHJWA_002102"
                     /gene_synonym="yebK"
     CDS             complement(8674..9543)
                     /gene="hexR"
                     /locus_tag="ACHJWA_002102"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFO4308699.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            9881..11356
                     /gene="zwf"
                     /locus_tag="ACHJWA_002103"
     CDS             9881..11356
                     /gene="zwf"
                     /locus_tag="ACHJWA_002103"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFO4308700.1"
                     /translation="MAVTQTAQVCDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            11591..13402
                     /gene="edd"
                     /locus_tag="ACHJWA_002104"
     CDS             11591..13402
                     /gene="edd"
                     /locus_tag="ACHJWA_002104"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFO4308701.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            13439..14080
                     /gene="kdgA"
                     /locus_tag="ACHJWA_002105"
                     /gene_synonym="eda"
     CDS             13439..14080
                     /gene="kdgA"
                     /locus_tag="ACHJWA_002105"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFO4308702.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(14136..15314)
                     /gene="purT"
                     /locus_tag="ACHJWA_002106"
     CDS             complement(14136..15314)
                     /gene="purT"
                     /locus_tag="ACHJWA_002106"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFO4308703.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYCESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            15448..15738
                     /gene="yebG"
                     /locus_tag="ACHJWA_002107"
     CDS             15448..15738
                     /gene="yebG"
                     /locus_tag="ACHJWA_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFO4308704.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            15805..16161
                     /gene="yebF"
                     /locus_tag="ACHJWA_002108"
     CDS             15805..16161
                     /gene="yebF"
                     /locus_tag="ACHJWA_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFO4308705.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            16488..17147
                     /gene="yebE"
                     /locus_tag="ACHJWA_002109"
     CDS             16488..17147
                     /gene="yebE"
                     /locus_tag="ACHJWA_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF533 domain-containing protein"
                     /protein_id="HFO4308706.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLIGGGAVAGTVLWNKYKDKIRAAHQDEPQFGSQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            17356..19416
                     /gene="ptrB"
                     /locus_tag="ACHJWA_002110"
     CDS             17356..19416
                     /gene="ptrB"
                     /locus_tag="ACHJWA_002110"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFO4308707.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRNHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDLQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     RPAD"
     gene            complement(19413..20075)
                     /gene="exoX"
                     /locus_tag="ACHJWA_002111"
     CDS             complement(19413..20075)
                     /gene="exoX"
                     /locus_tag="ACHJWA_002111"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFO4308708.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVHPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(20099..20755)
                     /gene="yobB"
                     /locus_tag="ACHJWA_002112"
     CDS             complement(20099..20755)
                     /gene="yobB"
                     /locus_tag="ACHJWA_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFO4308709.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEHGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            complement(20857..21087)
                     /gene="holE"
                     /locus_tag="ACHJWA_002113"
     CDS             complement(20857..21087)
                     /gene="holE"
                     /locus_tag="ACHJWA_002113"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFO4308710.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            21226..21600
                     /gene="yobA"
                     /locus_tag="ACHJWA_002114"
     CDS             21226..21600
                     /gene="yobA"
                     /locus_tag="ACHJWA_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFO4308711.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKRLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            21604..22476
                     /gene="copD"
                     /locus_tag="ACHJWA_002115"
     CDS             21604..22476
                     /gene="copD"
                     /locus_tag="ACHJWA_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFO4308712.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLTKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWKDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLLIISLLATLEPF"
     gene            22489..22830
                     /gene="yebY"
                     /locus_tag="ACHJWA_002116"
     CDS             22489..22830
                     /gene="yebY"
                     /locus_tag="ACHJWA_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFO4308713.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDVAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            23226..23882
                     /gene="pphA"
                     /locus_tag="ACHJWA_002117"
     CDS             23226..23882
                     /gene="pphA"
                     /locus_tag="ACHJWA_002117"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO4308714.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLSERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTFVQLQ
                     "
     gene            complement(23883..24074)
                     /gene="yebW"
                     /locus_tag="ACHJWA_002118"
     CDS             complement(23883..24074)
                     /gene="yebW"
                     /locus_tag="ACHJWA_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFO4308715.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(24179..24415)
                     /gene="yebV"
                     /locus_tag="ACHJWA_002119"
     CDS             complement(24179..24415)
                     /gene="yebV"
                     /locus_tag="ACHJWA_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFO4308716.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(24533..25972)
                     /gene="rsmF"
                     /locus_tag="ACHJWA_002120"
     CDS             complement(24533..25972)
                     /gene="rsmF"
                     /locus_tag="ACHJWA_002120"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFO4308717.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKTSVADFLRLTAPYGWTLTPIPWCEEGFWIERDDEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAALPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRSGGTLVYSTCTLNREENESVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPTPKYKVGNFPFSPVKD
                     REAAQIRQAAASVGLNWDGNLRLWQRDKEVWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNENAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTSNA"
     gene            complement(26053..28686)
                     /gene="yebT"
                     /locus_tag="ACHJWA_002121"
     CDS             complement(26053..28686)
                     /gene="yebT"
                     /locus_tag="ACHJWA_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFO4308718.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTNNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEACSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(28655..29938)
                     /gene="yebS"
                     /locus_tag="ACHJWA_002122"
     CDS             complement(28655..29938)
                     /gene="yebS"
                     /locus_tag="ACHJWA_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFO4308719.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVTSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            30068..30565
                     /gene="msrC"
                     /locus_tag="ACHJWA_002123"
     CDS             30068..30565
                     /gene="msrC"
                     /locus_tag="ACHJWA_002123"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFO4308720.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            30662..31360
                     /gene="proQ"
                     /locus_tag="ACHJWA_002124"
     CDS             30662..31360
                     /gene="proQ"
                     /locus_tag="ACHJWA_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFO4308721.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTAKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            31380..33428
                     /gene="prc"
                     /locus_tag="ACHJWA_002125"
     CDS             31380..33428
                     /gene="prc"
                     /locus_tag="ACHJWA_002125"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFO4308722.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            33620..34501
                     /gene="htpX"
                     /locus_tag="ACHJWA_002126"
     CDS             33620..34501
                     /gene="htpX"
                     /locus_tag="ACHJWA_002126"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFO4308723.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(34547..35920)
                     /gene="yebQ"
                     /locus_tag="ACHJWA_002127"
     CDS             complement(34547..35920)
                     /gene="yebQ"
                     /locus_tag="ACHJWA_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4308724.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQILTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACISGLRI
                     TQPRSRA"
     gene            36097..36888
                     /gene="kdgR"
                     /locus_tag="ACHJWA_002128"
     CDS             36097..36888
                     /gene="kdgR"
                     /locus_tag="ACHJWA_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFO4308725.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(37032..37271)
                     /gene="yobH"
                     /locus_tag="ACHJWA_002129"
     CDS             complement(37032..37271)
                     /gene="yobH"
                     /locus_tag="ACHJWA_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFO4308726.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAELGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            37431..37574
                     /gene="mgrB"
                     /locus_tag="ACHJWA_002130"
     CDS             37431..37574
                     /gene="mgrB"
                     /locus_tag="ACHJWA_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFO4308727.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            37649..37936
                     /gene="yebO"
                     /locus_tag="ACHJWA_002131"
     CDS             37649..37936
                     /gene="yebO"
                     /locus_tag="ACHJWA_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFO4308728.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVEDEDIIRLVAER"
BASE COUNT         9733 a         9369 c         9814 g         9113 t
ORIGIN      
        1 tgtgccattt ttcagttcat cgagacacct cgcaagtttt tttcatcctt cgctggatat
       61 ctatccagca tttttttatc atacagcatt atctttgatt cattacgcag gagcgtcatg
      121 tgataggcag actcagaggc atcatcattg aaaaacaacc cccgctggtg ttaattgaag
      181 tgggcggcgt aggctatgaa gtgcatatgc cgatgacctg tttttatgaa ctccctgaag
      241 cgggtcagga agcgatcgtt ttcacccact ttgtggtgcg tgaagacgcg caactgctgt
      301 acggttttaa caataaacaa gagcgcacgt tgttcaaaga gttgatcaaa accaacggcg
      361 tcggcccgaa gttggcgctg gcgatcctct ccggaatgtc agcgcagcag ttcgttaatg
      421 ccgttgagcg tgaagaagtg ggggcactgg tgaaactgcc gggtattggc aaaaaaaccg
      481 ccgaacgctt gattgttgaa atgaaagacc gatttaaagg tttgcatggc gatctcttta
      541 cgccagccgc cgacctggta ctcacgtctc ctgccagccc ggcgaccgac gatgctgaac
      601 aagaagcggt tgccgcgctg gtggcgctgg gctataaacc acaagaagca agccgcatgg
      661 tgagcaaaat cgctcgccct gacgccagca gtgaaacatt aattcgcgaa gccctacgcg
      721 ccgcgttatg aggtaaagga tgattgaagc agaccgtctg atttctgccg gtaccacttt
      781 gccggaagat gtagcagatc gcgccattcg ccctaaatta ctggaagagt atgttggtca
      841 gccgcaggtt cgttcacaga tggagatttt catcaaagca gcgaaactgc gcggcgatgc
      901 cctcgatcat ttgttgattt ttggtcctcc ggggttgggt aaaactacgc ttgccaacat
      961 tgtcgccaat gaaatgggcg ttaatttacg cacgacttct ggtccggtgc tggaaaaggc
     1021 gggcgatttg gctgcgatgc tcactaacct tgaaccgcat gacgtgctgt ttattgatga
     1081 gatccaccgt ttatcgccag ttgttgaaga agtgctgtac ccggcaatgg aagactacca
     1141 actggatatc atgattggtg aaggtccggc ggcacgctcc attaaaattg atttgccgcc
     1201 gtttaccctg attggtgcaa ccacgcgcgc aggttcgctg acatcaccgt tgcgcgaccg
     1261 ttttggtatt gtgcaacgtc tggagtttta tcaggtgccg gatctgcaat atatcgtcag
     1321 tcgcagcgca cgctttatgg ggcttgagat gagtgatgac ggcgcgctgg aagttgctcg
     1381 tcgcgctcgc ggtacgccgc gcattgccaa ccgtctgctg cgtcgagtgc gtgatttcgc
     1441 cgaagtgaag cacgatggca ccatctcggc agatatcgct gctcaggcgc tggatatgtt
     1501 gaatgtcgat gctgaaggtt tcgattatat ggaccgcaaa ttgttgctgg cggtaatcga
     1561 taagttcttt ggtgggccgg taggtctgga taaccttgcg gcagccattg gcgaagaacg
     1621 tgaaaccatt gaggatgtgc tggaacctta tttgattcag caaggctttt tgcagcgtac
     1681 accgcgtggg cgtatggcga cgacgcgggc gtggaatcac tttggcataa cgccgccaga
     1741 aatgccgtaa gtcggattgg tcgctcgcac cgcatccgac attggcagtg tgcctgatgc
     1801 gacgctattc gcgtcttatc aggcatatac cagagatagc gaaccgtagg tcggaaaagg
     1861 cgctcgcgcc gcatccgaca ttggcagtgt gcctgatgcg acgctgttcg cgtcttacca
     1921 ggcctaccag agatagcgaa ccgtaggtcg gataaggcgc tcgcgccgca tccgacaaat
     1981 gtgttcagcg atagattagc tggcctgctt tttcatcata ctgataataa ataacagtgc
     2041 cgcacatagc accaccgacg gacccgccgg cgtatcgtaa aacgcggaaa aggttaaacc
     2101 gtcagttact gccaccatcc ccaccaaaac agcgacacca gccatctgtt ccggcgtacg
     2161 ggcaaagcga cgcgcagtag ctgcaggaat aatcagcaac gaagtaataa tcaacgcacc
     2221 gacaaatttc atcgctacac caatcgtcaa tgccgtcacc agcatcaaca acaatttcac
     2281 tcgctgtaat ttcacaccat caacaaacgc cagatccggg ctaatcgtca tcgacagcaa
     2341 attgcgccat tgccagaaca aaatagccac cacgatgacc acgccaattg caatagagat
     2401 gagatcttct ggcgtcactg ccagtaaatc accgaacagg taagccatca aatcaacacg
     2461 aatgttagac atcagactaa cgaccaccag gcccagcgac agggcactgt gcgccataat
     2521 ccctaataac gtgtcgatcg ccagctgtgg acgcttctcc agccatacca gaccgcccgc
     2581 cagcagcagc gtaacggcaa tcaccgcata gaatggattc acgtccagca acaaaccaaa
     2641 cgcgacacca agtaatgagg catgagccag cgtatcaccg aaataagaca tacgacgcca
     2701 gactacaaac gaacccagcg gacccgcggc acaggcgagc atgatcccgg ctaaccaacc
     2761 gggaaataat aattcaatca tgagcgatca tttccccgac gcaaaacaat tcgtccctgt
     2821 aaatcgtgac gatgattatg atgatggcga tagataccca gttgttcagc accacgagga
     2881 ccaaacattg aaataaactc cggatgcagg gaaacaactt ccggtgtgcc ggaacaacaa
     2941 atgtgatgat tcaggcacag cacttcatcg gtttttgcca tcaccagatg aagatcgtga
     3001 gaaaccatta aaacgccaca atccagttca cgacgcagtt ggtcaataag gtcatataac
     3061 gccacctggc catttacatc cacgccttga gtgggttcat ccagcactaa taattgcggg
     3121 cgatttaaca atgctcgcgc taacagtaca cgctgcgttt cgccaccaga gagcttttgc
     3181 atcggtgcgt taatcaggtg cccggcctgg acacgtttca gtgcaggcaa aatatcttct
     3241 ttatgtgtac cagggcgtaa gcgtaaaaaa cggtttacgg tcagtggcaa cgtggtgtcg
     3301 agatacagct tctgcggtac atagccgatg cgcagttttc cgttgcgctt gataacccct
     3361 tcatcgggtg ttaccagccc gagcactacc cgtaccagtg tcgacttacc tgcgccattt
     3421 ggcccaagta aagtcaaaat ttttccaggt ttaagttcca gcgacacatc agagaggacg
     3481 cggcgttggc caaaagaaac cgagacattt tccagggaaa ccagacttgt catgttaatt
     3541 ttagtcttgc agtagtcatg aaatgttata atatcacact tctcatattc attacgatga
     3601 ttggtcgcat tatgttacat aaaaaaacgc ttcttttcgc agcattattc gccgctctct
     3661 ggggcggtgc aacacaggcc gcagatgccg ccgttgtcgc ttcgcttaaa cccgttgggt
     3721 tcatcgcttc tgccattgct gatggggtaa cagaaacgga ggttttactt cctgacggcg
     3781 cttcagaaca tgattattca ctgcgtccat cggatgtaaa acgcttacag aacgcggact
     3841 tagtcgtttg ggttggcccg gagatggaag cgtttatgca aaaaccggta agcaaattac
     3901 cagaagcgaa gcaggtaacg attgcgcagc ttgaagatgt gaaaccgcta ctgatgaaaa
     3961 gtattaacgg cgatgatgat gatcacgacc acgcggaaaa aagtgacgaa gatcaccatc
     4021 acggcgattt caacatgcat ctttggcttt ccccagagat agcgcgggct acagcggttg
     4081 caatccatgg aaaattagtg gaacttatgc cgcaaagtcg agccaaactt gacgccaacc
     4141 tgaaggattt tgaggcacaa ttagcctcaa ccgaaacgca ggttggtaac gagctcgcgc
     4201 cgctcaaggg gaaaggttat ttcgtttttc acgatgctta cggctatttc gaaaaacagt
     4261 tcggactgac accgcttggt cattttaccg ttaaccctga gattcaacct ggcgcgcagc
     4321 gtttacatga aataagaaca cagttggttg agcaaaaagc aacctgcgtt tttgctgagc
     4381 cacagttcag gccagcggtc gttgagagcg tcgcacgagg gacatccgtt cgtatgggaa
     4441 cgttggatcc cttggggacg aatatcaaac tgggtaaaac aagctattca gaattcctga
     4501 gtcaattagc caaccagtat gcgagctgcc tgaaaggaga ttaatgagga agtgattacg
     4561 tgcaacagat agcccgctct gtcgccctgg cgtttaataa tttaccgcga ccacaccgcg
     4621 ttatgttggg gtcgctcacc gttcttactc tggccgtcgc tgtctggcgg ccctatgttt
     4681 atcaccgcga tgccacgcca attgtcaaaa ccattgagct ggaacagaac gaaattcgtt
     4741 cgctcttacc tgaagccagt gagccgattg atcaagctgc acaagaagat gaagccattc
     4801 cccaggacga actggatgac aaaatcgccg gtgaagcggg cgtgcatgaa tatgttgttt
     4861 ccactggcga tacgctaagc agcattctca atcagtatgg tattgatatg ggtgatatca
     4921 cccaactggc tgcggccgac aaagaattgc gtaacctgaa aatcggtcaa caactctcct
     4981 ggacattaac cgcggacggc gaactgcagc gcctcacctg ggaagtgtct cgtcgtgaaa
     5041 cccgaaccta tgaccgtact gccgctaacg gttttaaaat gaccagcgaa atgcagcaag
     5101 gagagtgggt taacaatctg ctgaaaggta ccgtcggggg gagctttgtt gccagcgcca
     5161 gaaacgccgg tttgaccagc gccgaagtga gcgcagtgat taaagccatg cagtggcaaa
     5221 tggatttccg caaactgaaa aaaggcgatg aatttgcggt attaatgtct cgagaaatgc
     5281 ttgatggtaa acgtgagcaa agccagctgc tgggcgtacg tttgcgttca gaaggtaaag
     5341 attattacgc tatccgcgct gaagatggca aattctacga tcgtaacggt actggtctgg
     5401 cgaaaggatt cttgcgattc ccgacggcga aacagttccg tatctcgtct aactttaacc
     5461 cgcgtcgtac taatccggtg accggtcgcg ttgcaccaca cagaggtgtt gatttcgcca
     5521 tgccgcaggg tacgccagtg ctttcagtgg gtgacggtga agtggtggtt gccaaacgca
     5581 gtggcgcagc aggttattat gtggctattc gtcatggtcg cagctacacc acgcgttata
     5641 tgcacttgcg caagattctg gtgaaaccgg ggcagaaggt gaaacgtggc gaccgtatcg
     5701 cgctttccgg taataccgga cgttcaaccg ggccgcatct gcactatgaa gtatggataa
     5761 accagcaggc cgtaaacccg ctgacggcaa aactgccgcg taccgaaggg ctgaccggct
     5821 ccgatcgtcg cgaattcctg gcgcaggcca aagagattgt gccgcagcta cggtttgatt
     5881 aattaacatc cattcgcagc cggtacgcag tcagtaccgg ctttttttat ttggtgcggg
     5941 gcaagttgcg ccgctacact atcaccagat tgatttttgc cttatccgaa actggaaaag
     6001 catggaaacg aaaaaaaata atagcgaata cattcctgag tttgataaat cctttcgcca
     6061 cccgcgctac tggggagcat ggctgggcgt agcagcgatg gcgggtatcg ctttaacgcc
     6121 gccaaagttc cgtgatccca ttctggcacg gctgggacgt attgccggac gactgggaaa
     6181 aagctcacgc cgtcgtgcgt taatcaatct gtcgctctgc tttccagaac gtagtgaagc
     6241 tgaacgcgaa gcgattgttg atgagatgtt tgccaccgcg ccgcaagcga tggcaatgat
     6301 ggctgagttg gcaatacgcg ggccggagaa aattcagccg cgcgttgact ggcaagggct
     6361 ggagatcatc gaagagatgc ggcgtaataa cgagaaagtt atctttctag tgccgcacgg
     6421 ttgggccgtc gatattcctg ccatgctgat ggcctcgcaa gggcagaaaa tggcagcgat
     6481 gttccataat cagggcaacc cggtttttga ttatgtctgg aacacggtgc gtcgtcgctt
     6541 tggcggtcgt ctgcatgcga gaaatgacgg tattaaacca ttcatccagt cggtacgtca
     6601 ggggtactgg ggatattatt tacccgatca ggatcatggc ccagagcaca gcgaatttgt
     6661 ggatttcttt gccacctata aagcgacgtt gcccgcgatt ggtcgtttga tgaaagtgtg
     6721 ccgtgcgcgc gttgtaccgc tgtttccgat ttatgatggc aagacgcatc gtctgacgat
     6781 tcaggtgcgc ccaccgatgg atgatctgtt agaggcggat gatcatacga ttgcgcggcg
     6841 gatgaatgaa gaagtcgaga tttttgttgg tccgcgacca gaacaataca cctggatact
     6901 aaaattgctg aaaactcgca aaccgggcga aatccagccg tataagcgca aagatcttta
     6961 tcccatcaaa taaaaaagcc tctcgcgagg agaggccttc gcctgatgat aagttcaagt
     7021 ttgcttcaga atattcgaaa tctgttgaac tatcattgaa ctgtaggccg gatgtggcgt
     7081 tttcgccgca tccggcaacg tacttactct accgttaaaa tacgcgtggt attagtagaa
     7141 cccacggtac tcatcacgtc gccctgggtg acaatcacca ggtcaccaga catcaagtaa
     7201 cctttatcgc gcagcagatt aaccgcttcg ctggcagctg ctacgccgtc attagcgcta
     7261 tcaaagtgca ccggcgtaac gccacgatag agagcagtca ggttcagcgt acgttcatgg
     7321 cgcgacatgg cgaaaattgg tagaccagag ctgatacggg aggtcatcag cgcggtacga
     7381 cccgattcgg tcatggtaat gatcgccgta acgcctttca ggtggttagc tgcgtacatt
     7441 gctgacatgg caatagcttc ttccacattg tcgaactgaa cgtccagacg gtgtttagaa
     7501 acgttgatgc tcgggatttt ttccgcaccc aggcaaacgc gcgccatggc tgcaacagtt
     7561 tctgacggat actgcccagc ggcagtttct gcagacagca tcacagcgtc agtaccatcc
     7621 agaacggcgt ttgctacgtc catgacttct gcacgcgtcg gcatcgggtt agtaatcatt
     7681 gactccatca tctgggtcgc cgtgattacc gctcggttta gctgacgcgc acgacggatc
     7741 aacgctttct gaatgccgac cagttccggg tcgccaattt ccacaccgag gtcgccacgt
     7801 gcaaccatta ccacgtcaga ggcgaggatg atgtcatcca ttgcatcctg gctgcaaacg
     7861 gcttccgcac gttcaacctt ggcaacaatt ttcgcatcac atcctgcatc gcgtgccagg
     7921 cgacgggcat agttcagatc ttcgccacag cgtgggaagg agacagccag gtaatctacg
     7981 ccaatcaacg ccgcagtctt aatgtctgct ttgtcttttt cggtcagcgc ttcagccgac
     8041 aaaccgccgc caagtttgtt gataccttta ttgttggaga ggggaccacc gacggtgact
     8101 tcggtgaaca ctttcatgcc ctgaacttcc agtactttta actggacgcg accatcgtcc
     8161 agcagcagga tgtcaccagg cacgacgtca gcaggcaggc ctttgtagtt gataccgact
     8221 ttttctttgt cgccttcacc tttacccagg ttggcgtcga gcaggaattt atccccaata
     8281 ttgaggaaaa ctttgccttc tttaaaggtg gatacacgga ttttgggccc ctggaggtca
     8341 cccagaatag ccacatgacg ccccagtttt gcggcaatct cacgaacttt atccgcgcgc
     8401 attttgtgat cttcaggcga gccgtgagaa aagttcatac gtacaacgtt ggcacccgcc
     8461 gcgataactt tttcaagatt attatcgcga tctgttgctg ggcctaacgt ggtaacgatt
     8521 tttgttctgc gaagccttct ggacatgtaa tactccgttg actgaaacaa ccaggtgttg
     8581 cttgaacatg aaatccgaat gaaataacgc cgcgatggcg ataaaataat ctcattcgga
     8641 tgtcgtataa gattagaaca gtgacagtcg cttttagcga tcgtcactta aattaagtaa
     8701 ctgcttatca aaacgcgatt ccttcagcgc ttccttgacc cgcttcaagt tatctctgaa
     8761 ttttgcaccg cgtcgcaaag taaatcctgt cgccagcaca tctatcacgg tcagctgtgc
     8821 aagtcgagaa accatgggca tataaatgtc agtatcttcc ggtacgtcga gggtaattgc
     8881 cagcgttgct tcccgggcga gcggggtacc cgcagaggtg agggcaatca ccatagcgtc
     8941 gttttcgcgt gccagctgcg ccagctcgac cagatttttt gttcttccgg tgtgagaaat
     9001 cagcaccacc acgtctccgt cgctacaatt catacaactc atgcgttgca gcacaatatc
     9061 atcggagtac accaccggaa cattaaaacg aaagaactta ttcatcgcat cgtgggcaac
     9121 ggcggctgaa gagcctaatc cgaaaaaggc gatttttttt gcctgagtga gcaagtcgac
     9181 ggcgcggttg atggcagatt tatccagtga atgacggaca tgatcgagcg ttgccattgc
     9241 ggactcaaat attttccctg tgtatgattc aacgctgtca tcttcattga cgttgcgatt
     9301 aacataggga gtgccattcg ccagactctg tgccagatga agtttaaaat caggaaaacc
     9361 gcgcgtgtcc atgctgcgac agaaacgatt caccgtcggt tcgctaacat tggcttccag
     9421 tgccagagca gcaatactcg aatggatcgc gttatcgggc gaagccagaa tgacctcggc
     9481 aactttgcgc tctgatttgc tcaaatgttc cagctgagac tggatttttt ccagcatatt
     9541 catgatgtaa agagactcac gggtaatgac gatttccgca ctgaaagaaa tcgaaatgca
     9601 gttttgtcag atattacgcc tgtgtgctgt gttaatgaca aaagcagata aaaaagttgt
     9661 tatttttttt cataacatga tcagtgtcag atttttaccc aatggaaaac gatgattttt
     9721 ttatcagttt tgccgcactt tgcgcgcttt taccgtaatc gcacgggtgg ataagcgttt
     9781 acagttttcg caagctcgta aaagcagtac agtgcaccgt aagaaaatta caagtatacc
     9841 ctggcttaag taccgggtta gttaacttaa ggagaatgac atggcggtaa cgcaaacagc
     9901 ccaggtctgt gacttggtca ttttcggcgc gaaaggcgac cttgcgcgtc gtaaattgct
     9961 gccttccctg tatcaactgg aaaaagccgg tcagctcaac ccggacaccc ggattatcgg
    10021 cgtagggcgt gctgactggg ataaagcggc atataccaaa gttgtccgcg aggcgctcga
    10081 aactttcatg aaagaaacca ttgatgaagg tttatgggac accctgagcg cacgtctgga
    10141 tttttgtaat ctcgatgtca atgacactgc tgcattcagc cgtctcggcg cgatgctgga
    10201 tcaaaaaaat cgtatcacca ttaactactt tgccatgccg cccagcactt ttggcgcaat
    10261 ttgcaaaggg cttggcgagg caaaactgaa tgctaaaccg gcacgcgtag tcatggagaa
    10321 accgctgggg acgtcgctgg cgacctcgca ggaaatcaat gatcaggttg gcgaatactt
    10381 cgaggagtgc caggtttacc gtatcgacca ctatcttggt aaagaaacgg tgctgaacct
    10441 gttggcgctg cgttttgcta actccctgtt tgtgaataac tgggacaatc gcaccattga
    10501 tcatgttgag attaccgtgg cagaagaagt ggggatcgaa gggcgctggg gctattttga
    10561 taaagccggt cagatgcgcg acatgatcca gaaccacctg ctgcaaattc tttgcatgat
    10621 tgcgatgtct ccgccgtctg acctgagcgc agacagcatc cgcgatgaaa aagtgaaagt
    10681 actgaagtct ctgcgccgca tcgaccgctc caacgtacgc gaaaaaaccg tacgcgggca
    10741 atatactgcg ggcttcgccc agggcaaaaa agtgccggga tatctggaag aagagggcgc
    10801 gaacaagagc agcaatacag aaaccttcgt ggcgatccgc gtcgacattg ataactggcg
    10861 ctgggccggt gtgccattct acctgcgtac tggtaaacgt ctgccgacca aatgttctga
    10921 agtcgtggtc tatttcaaaa cacctgaact gaatctgttt aaagagtcgt ggcaggatct
    10981 gccgcagaat aaactgacta tccgtctgca acctgatgaa ggcgtggata tccaggtact
    11041 gaataaagtt cctggccttg accacaaaca taacctgcaa atcaccaagc tggatctgag
    11101 ctattcagaa acctttaatc agacgcatct ggcggatgcc tatgaacgtt tgctgctgga
    11161 aaccatgcgt ggtattcagg cactgtttgt acgtcgcgat gaagtggaag aagcctggaa
    11221 atgggtagac tccattactg aggcgtgggc gatggacaat gatgcgccga aaccgtatca
    11281 ggccggaacc tggggacccg ttgcctcggt ggcgatgatt acccgtgatg gtcgttcctg
    11341 gaatgagttt gagtaatatc tgcgcttatc ctttatggtt attttaccgg taacatgatc
    11401 ttgcacagat tgtagaacaa tttttacact ttcaggcctc gtgcggattc acccacgagg
    11461 ctttttttat tacactgact gaaacgtttt tgccctatga gctccggtta caggcgtttc
    11521 actcataaat cctctgaatg aaacgcgttg tgaatcatcc tgctctgaca actcaatttc
    11581 aggagccttt atgaatccac aattgttacg cgtaacaaat cgaatcattg aacgttcgcg
    11641 cgagactcgc tctgcttatc tcgcccggat agaacaagcg aaaacgtcga ccgttcatcg
    11701 ttcgcagttg gcatgcggta acctggcaca cggtttcgct gcctgccagc cagaagacaa
    11761 agcctctttg aaaagcatgt tgcgtaacaa tatcgccatc atcacctcct ataacgacat
    11821 gctctccgct caccagcctt atgaacacta tccagaaatc attcgtaaag ccctgcatga
    11881 agcgaatgcg gttggtcagg ttgcgggcgg tgttccggcg atgtgtgatg gtgtcaccca
    11941 ggggcaggat ggaatggaat tgtcgctgct aagccgcgaa gtgatagcga tgtctgcggc
    12001 tgtcgggctg tcccataaca tgtttgatgg tgctctgttc ctcggggtgt gcgacaagat
    12061 tgtcccgggt ctgacgatgg cagccctgtc gtttggtcat ttgcctgcgg tgtttgtgcc
    12121 gtctggaccg atggcaagcg gtttgccaaa taaagaaaaa gtgcgtattc gccagcttta
    12181 tgccgaaggt aaagtggacc gcatggcctt actggagtca gaagccgcgt cttaccatgc
    12241 gccgggaaca tgtactttct acggtactgc caacaccaac cagatggtgg tggagtttat
    12301 ggggatgcag ttgccaggct cttcttttgt tcatccggat tctccgctgc gcgatgcttt
    12361 gaccgctgcc gctgcgcgtc aggttacacg catgaccggt aatggtaatg aatggatgcc
    12421 gatcggtaag atgatcgatg agaaagtggt ggtgaacggt atcgttgcac tgctggcgac
    12481 cggtggttcc actaaccaca ccatgcacct ggtggcgatg gcgcgcgcgg ccggtattca
    12541 gattaactgg gatgacttct ctgacctttc tgatgttgta ccgctgatgg cacgtctcta
    12601 cccgaacggt ccggccgata ttaaccactt ccaggcggca ggtggcgtac cggttctggt
    12661 gcgtgaactg ctcaaagcag gcctgctgca tgaagatgtc aatacggtgg caggctttgg
    12721 tctgtctcgt tatacccttg aaccatggct gaataatggt gaactggact ggcgggaagg
    12781 ggcggaaaaa tcactcgaca gcaatgtgat cgcttccttc gaacaacctt tctctcatca
    12841 tggtgggaca aaagtgttaa gcggtaacct gggccgtgcg gttatgaaaa cctctgccgt
    12901 gccggttgag aaccaggtga ttgaagcgcc agcggttgtt tttgaaagcc agcatgacgt
    12961 tatgccggcc tttgaagcgg gtttgctgga ccgcgattgt gtcgttgttg tccgtcatca
    13021 ggggccaaaa gcgaacggaa tgccagaatt acataaactc atgccgccac ttggtgtatt
    13081 attggaccgg tgtttcaaaa ttgcgttagt taccgatgga cgactctccg gcgcttcagg
    13141 taaagtgccg tcagctatcc acgtaacacc agaagcttac gatggcgggc tgctggcaaa
    13201 agtgcgcgac ggggacatca ttcgtgtgaa tggacagaca ggcgaactga cgctgctggt
    13261 agacgaagcg gaattggctg ctcgcgaacc acacattcct gacctgagcg cgtcacgcgt
    13321 gggaacagga cgtgaattat tcagcgcctt gcgtgaaaaa ctgtccggtg ccgaacaggg
    13381 cgcaacctgt atcacttttt aagacgacaa atttgtaatc aggcgagaga aaactctgat
    13441 gaaaaactgg aaaacaagtg cagaatcaat cctgaccacc ggcccggttg taccggttat
    13501 cgtggtaaaa aaactggaac acgcggtgcc gatggcaaaa gcgttggttg ctggtggggt
    13561 gcgcgttctg gaagtgactc tgcgtaccga gtgtgcagtt gacgctatcc gtgctatcgc
    13621 caaagaagtg cctgaagcga ttgtgggtgc cggtacggtg ctgaatccac agcagctggc
    13681 agaagtcact gaagcgggtg cacagttcgc aattagcccg ggtctgaccg agccgctgct
    13741 gaaagctgct accgaaggga ctattccgct gattccgggg atcagcactg tttccgaact
    13801 gatgctgggt atggactacg gtttgaaaga gttcaaattc ttcccggctg aagctaacgg
    13861 cggcgtgaaa gccctgcagg cgatcgcggg tccgttctcc caggtccgtt tctgcccgac
    13921 gggtggtatt tctccggcta actaccgtga ctacctggcg ctgaaaagcg tgctgtgcat
    13981 cggtggttcc tggctggttc cggcagatgc gctggaagcg ggcgattacg accgcattac
    14041 taagctggcg cgtgaagctg tagaaggcgc taagctgtaa aagtcaaaat gcccgatcga
    14101 ggatcgggca tttttgtagc gtttatttgc cgggtttaac cctgtacttt tacctgtccg
    14161 gcggcgtgct tcgcgcgttc aatggcgtca acaacactct ctgcagtagc cagtgccacc
    14221 cccagacgac ggctgccatc aatttccggc ttaccaaata aacgaatctg caaatctgcg
    14281 cctacggcat tctgcacatt atcaaacgtg acattctgac tggtcagttg tggcagaata
    14341 acggcagaag ctgcaggacc atactgacgg atcccgccaa ccggaagtcc gaggaaggca
    14401 cgtacatgca gggcaaactc tgagagatct tgagaaatta acgtcaccat cccggtatca
    14461 tgtggacgag gggagacctc actgaaaatc acctcatcac cacagacaaa cagctcgaca
    14521 ccaaacagac cataaccgcc cagtgccagc accactttac gggcaatctc ctgcgcacgt
    14581 tcaagggcaa gcgggctcat ttgctgtggt tgccaggatt cacagtagtc gccatcttcc
    14641 tggcgatgac ctactggtgc acagaaatgg acgccatcca ccgcgctgac ggttagcagg
    14701 gtaatttcga agtcaaactt aacgacgcct tcaacaatta cgcggcccgc tccggcgcga
    14761 ccgccttgct gagcgtattc ccatgcctga gcaagttgct ctgcagagcg aataaacgtc
    14821 tgccccttgc cggaagagct catcaccggt tttacaatgc agggatagcc aatggcggca
    14881 accgcctcgc ggaaaaggct ttcgctatcg gcaaaacgat aagtggaagt gggcagctgc
    14941 agctcttctg ccgccaggcg acggataccc tcgcgattca tcgttaattt cgttgcgcga
    15001 gcgcagggga caacattcag tccttcctct tcaagttgga tcagcatatc ggtggcaata
    15061 gcttcgatct ccggcacgat ataatgtggt ttttccagtt caaccacacg gcgtaatgca
    15121 tcaccatcaa gcatattaat gacatgggag cgatgcgcga catgcatggc tggtgcgtcg
    15181 gcatagcgat cgacggcaat cacctctacg ccgagacgct gacactcgat tgccacttct
    15241 ttacccagtt caccggagcc taataacatc acgcgagttg ctgccggacg cagcgcagtg
    15301 cctaataacg tcataacgaa tatcccctga ttaattccgc gcgcagtata aacgaaaacg
    15361 tttgcgtgtg tctttattgc tggtgttgat ctctcaaccg aaaagaaata tactgtatag
    15421 aatcacagtt attatgagag gctgcaaatg gcggttgaag ttaaatacgt agtcattcgt
    15481 gagggcgaag agaaaatgtc gtttaccagc aaaaaggaag ccgatgctta tgacaaaatg
    15541 cttgatacgg cggatctgct tgacacctgg ctgacaaatt ctccagtgca aatggaagac
    15601 gagcaacgtg aagccctttc gctatggctg gcagaacaaa aagatgtgct gagcaccatt
    15661 ctgaaaaccg gcaaattacc gtccccacag gtcgtcggtg ctgaatccga agaggaagat
    15721 gcctcacacg cggcctgaag caacttgcgc tcctgacaat ttgtaccatg ctttctcctg
    15781 aatcattgtg ctatggagaa aaacatgaaa aaaagagggg cgtttttagg gctgttgttg
    15841 gtttctgcct gcgcatcagt tttcgctgcc aataatgaaa ccagcaagtc ggtcactttc
    15901 ccaaagtgtg aaggtctgga tgctgccgga attgccgcga gcgtaaaacg tgattatcaa
    15961 caaaatcgcg tggcgcgctg ggcagatgat caaaaaattg tcggtcaggc cgatcccgtg
    16021 gcatgggtca gtttgcagga cattcagggt aaagatgata aatggtcagt accgctaacc
    16081 gtacgtggta aaagtgccga tattcattac caggtcagcg tggactgcaa agcgggaatg
    16141 gcggaatatc agcggcgtta atgggttctg ataattcgat tgaccgttgg cggatgcggc
    16201 gcgagtgcct tgtccgcccc agaacgcacc acaatcgtag gcctgagaag acgcaacagc
    16261 gtcgcatcgg gcaatcagca tcaaacaatg ccactcaaaa cgacaaacta ctttcaccta
    16321 aaataccctt atcacctgat atacagtaaa taccttacct ggcgcgaatt tctttgaata
    16381 acggcctacc gccccgctta tttgcgcatc ttttgtctcg atatacattt tctgacatcc
    16441 tcatcctcta tgctacaggc actaaaaatg gttattgagg agcaaatatg gctaactggt
    16501 taaatcaact gcaatccctg cttgggcaaa gcagttcttc tacctcctcg tctgcggatc
    16561 agggattggg caaactttta gtgccgggcg cattaggcgg actggctgga ctgctggtcg
    16621 caaataaatc agcacgtaaa cttcttacga aatatggcac caacgcgttg ctgattggcg
    16681 gcggagcggt agcgggtacg gtgctgtgga ataaatacaa agataaaatt cgcgcggcgc
    16741 atcaggacga accgcagttt ggctcgcaaa gtacgccgct ggatgagcgt acggaacgtt
    16801 tgatccttgc gctagtcttt gccgctaaaa gtgatggtca tattgatgcc aaagaacgtg
    16861 cggcaatcga ccagcaattg cgtgaagccg gcgtggaaga gcaggggcgg gtactcattg
    16921 agcaggcaat cgaacaaccg ctggatccac aacgcctggc taccggggtc cgcaatgaag
    16981 aagaggcgct ggaaatctat ttcctgagct gcgcggctat tgatattgac cattttatgg
    17041 aacgcagtta cctgaatgca ctgggtgacg cgctgaaaat cccccaggat gttcgtgaag
    17101 gcattgaacg cgatctcgaa cagcaaaaac gcactttggc ggaataattc cgctctctgg
    17161 atagcctgaa gttgactatt tcgggctatc cagaacagca cagccatttg aacaggctga
    17221 ttttctctgt ctgcataaca ttcgatattc gcgccattac ctgtcatcat ctaagcaatg
    17281 actgccctgt ttcgcttgca tccccggtga gttttgccac ccttataaga tgtttcaacc
    17341 agaaagaaca ataacatgct accaaaagcc gcccgcattc cccacgccat gacgcttcat
    17401 ggcgatacgc gcatcgataa ttactactgg ctgcgggacg atacgcgttc tcagccggaa
    17461 gtcctggact acctgcaaca agaaaatagt tacggtcatc gggtgatggc ctcacaacaa
    17521 gccttgcagg atcgcatctt aaaggaaatc atcgaccgca ttccgcaacg agaagtttct
    17581 gcgccctaca tcaaaaatgg ctaccgctac cggcatattt atgaaccagg ctgtgaatat
    17641 gctatctacc agcgtcaatc ggcgttcagt gaagagtggg acgagtggga aatattgctc
    17701 gatgccaaca agcgcgcggc tcatagtgag ttttattcga tgggcggaat ggcgattacg
    17761 cccgataaca ccattatggc gctggcagaa gattttcttt cccgacgcca gtacggcatt
    17821 cgttttcgta atctggaaac aggtaactgg tacccggaac tgctggataa cgttgaaccc
    17881 agctttgtct gggcaaatga ctcctggact ttctactatg ttcgcaagca tccggtgacg
    17941 ctgctgcctt atcaggtctg gcgtcacgcc atcggtacgc cagcatcgca agataaactg
    18001 atttacgaag aaaaagacga tacctattac gtcagcctgc ataaaacgac ctcaaagcac
    18061 tatgtagtca ttcatttggc cagcgccacc accagtgaag ttcgcctgct ggacgcggaa
    18121 atggcagatg ccgagccgtt tgtttttctg ccgcgccgca aagatcacga atacagcctt
    18181 gatcactacc agcatcgttt ttatctgcgt tccaaccgcc acggcaaaaa ctttggctta
    18241 taccgtaccc gtatgcgtga tgagcaacag tgggaagagt taattccgcc acgcgaaaac
    18301 atcatgctgg aagggtttac gctgtttacc gactggctgg tggttgaaga gcgtcagcgc
    18361 gggttaacca gtttgcgcca aattaaccgc aagacccggg aagtcattgg tattgccttt
    18421 gatgatccag cctatgtgac ctggattgcc tacaatccag aacctgaaac cgcgcgattg
    18481 cgttatggtt attcttccat gaccacacca gacactttgt ttgaactgga tatggatacc
    18541 ggtgagcgtc gtgtattaaa acaaacagaa gttcctggtt ttgatgcggc gaattaccgc
    18601 agtgaacacc tgtggatagt cgcccgtgat ggtgtcgaag ttccggtttc gttggtctac
    18661 catcgcaatc attttcgcaa aggacacaac ccgctgctgg tgtatggcta cggttcttac
    18721 ggcgcaagta ttgatgccga tttcagtttt agccgcttga gtttgttaga tcgtggcttt
    18781 gtctacgcca ttgtccatgt tcgcggcggc ggtgagctgg ggcaacaatg gtacgaagac
    18841 ggtaaatttc tgaagaagaa aaatacgttt aatgattatc ttgatgcctg cgatgcattg
    18901 ttaaaactgg gctatggctc tccttcgctt tgttatgcga tgggcgggag tgcggggggc
    18961 atgttgatgg gcgttgcaat taatcaacgc ccggaattat tccacggcgt tatcgcccag
    19021 gtaccgtttg ttgatgttgt aacaacgatg cttgatgaat caattcctct taccactggt
    19081 gagtttgaag agtgggggaa tccgcaggat ctgcaatatt acgagtatat gaaaagctac
    19141 agcccgtatg acaacgtcac cgcacaggct tatccacatt tactggtaac gaccggttta
    19201 cacgattctc aggtgcaata ttgggaaccg gcaaaatggg tcgctaagtt gcgcgagctg
    19261 aaaaccgata accatctttt attgctctgt accgacatgg actcaggcca tggcggtaaa
    19321 tctggtcgct ttaaatcgta cgaaggcgta gcgatggaat atgcttttct ggtcgcgctg
    19381 gcgcagggaa cattacccgc taggcctgcg gattaggtat tttccagata atgtttcagt
    19441 gttaaacgca gctccgggct catgctgtcc aggttattaa acaaccagcg cagatagccc
    19501 ggatcgcgtt cggcaacgtc ggaaaccgct ttgccacggt atttgccaaa ggtgaaggtc
    19561 gtcatcaacg acggacgtcc ggttatatcg gccatctgtt ctgccgtcca gccggaggtg
    19621 ttcataatat cgataagcaa cgcggcggtg atataacagt catacaacgc gcggtgatga
    19681 tgcaggcccg gcggcgtctg tacattgagc ttccgtgttt tatataacgc catattgctg
    19741 tacttgatcc ctggccacaa acgacgggcc agtttcatag tgcaaatcca ctcaccgggc
    19801 atctcaggca gtacgcggcg gtcaaagctg gcgttatgcg cgacatacca ttcactaccg
    19861 taatagtgtg ggatcacatc ttcaatccac ggtttatcgg cgaccatggc ttcggtgatg
    19921 cgatgaatcg ccatcgcttg tggactaata ggacgatcgg ggtgcaccag gtggctcatg
    19981 gggttgacga ttttcccgtc aatgacatca acagaggcaa tctcaacgat ccctccctgc
    20041 aaaccgcagg tttctgtatc gataatgcgc aacatggcat gctccaggcc gaaaaagcct
    20101 agcgtaatgg aatgatatcg ccttgccaac cctgtgatga acgctgaccg actaacaata
    20161 atgaaccacg atcggcgcga acgatgagac gaccatgttc atcccatagt gcactgttac
    20221 cacgggcatt tgccatcagc acagcaatcg agtactgatg tgaaaaaaat tgtaagcgac
    20281 gggccgatgc cagcaggtcg ggttctccca gacattgccc tgtcgtaaac agtgaacagg
    20341 tcgggtccat atccatacct tgcggttgtt catcgaccac cgtgatggtt ctggaacgac
    20401 ggcccagaca tgcaccatga ctctgatggt agatcccagg cgtttttcgc cacggtgcga
    20461 acaccgcaat tccacgaata aagcgatcgt tatattcaac gggaaggccg gcaatgatgg
    20521 tcattcgcca ggtcgttgcg gcataacaaa gcgggtctaa cagtgagaga tcgggtgggg
    20581 caggaagggc acgtcgcgaa taatcacacc ccagtaaaga aagcgatgga aaaactaaca
    20641 gctgacattg ttgcctggcg gcggccctca caaactccag atgatgagct acctgttcgg
    20701 tgagtgaggt tttacgaggc tcatattgcg ctgctgcaac tttccagaac gacataatga
    20761 cttccttttc aaaatgactg caaattatgg gtgcggttta gaaaatactc ataatttagc
    20821 aggcgttatg taagaaagtg taactctgta taagttttat ttaagtttgg gctcgtaagg
    20881 taaacgtgac agattgaccg aagccaaacg gtgggcaata agccgctcgc gaaaccagct
    20941 gcgcaaatgt tcaggctgtt cacgttcaac cgcttcagcg atcactggca tattgtagcg
    21001 ttctttaaat gccaccccgg ccgccgccaa atcgacattc actttatcca tttctgtttg
    21061 atccagttta gccagattct tcagcattct taatctcctc atcattcgcg ggcaaaagtt
    21121 actaagagtg agcctggatg gcaagccgga attgatgatc ctcgactcag acattagaca
    21181 aggttatgct ctgtaaaaaa gttattacaa aaaggaatag ataatatggc ttcaactgca
    21241 cgctcccttc gctacgcgct ggcaatcctg accacttcgt tggtgacacc ttccgtctgg
    21301 gcacatgcgc atttaacgca tcagtatcct gcggcaaacg cgcaagtgac agctgcaccg
    21361 caggcaatca ctttaaactt ctcggaaggt gttgaaacag gattcagtgg tgcaaaaatc
    21421 acggggccaa aaaacgaaaa tattaaaaca ttaccggcga agcgaaatga gcaggaccaa
    21481 aagcgactga ttgttccact ggccgattcg ctgaaacccg gaacctacac cgttgactgg
    21541 catgttgttt cggtggacgg gcataaaacg aaaggacact acacctttag cgtgaaataa
    21601 atcatgctgg cgtttacctg gatcgcgctg cgatttatcc atttcacctc gctgatgctg
    21661 gtctttggct tcgcaatgta cggtgcctgg ctggcaccct taacgattcg tcgcttgctg
    21721 acgaagcgtt ttctgcgttt acaacagcac gctgccgtct ggagtttgat cagtgccact
    21781 gcaatgctcg ccgttcaggg cgggttgatg ggaacgggat ggaaagatgt attttcgcca
    21841 aacatttggc aggctgtttt gcaaacgcag tttggtggcg tctggttatg gcaaattgtt
    21901 ctcgccctcg taacgttgat cgtcgccctt atgcaaccgc ggaatatgcc acgtttgctg
    21961 tttatgctca ctaccgcgca atttattctg ctggcgggag tggggcatgc gacgctgaat
    22021 gaaggggtaa cggcgaaaat ccatcagact aatcacgcga ttcacctgat ttgtgcggcg
    22081 gcgtggtttg ggggattact gccggtgctc tggtgtatgc aactcatcaa aggccgttgg
    22141 cgacaccagg ctattcaggc gctgatgcgt ttttcctggt gcgggcattt tgcggtgatc
    22201 ggcgtactgg caagcggcgt gcttaatgca ttgttgatta ccggatttcc ccccacgctt
    22261 acgacttact ggggccaact cttattgctc aaagccatcc tggtgatgat tatggtggtc
    22321 attgcgctgg ctaatcgtta tgttcttgta ccgcgcatga ggcaggatga agatcgtgcg
    22381 gcaccctggt ttgtgtggat gactaaactc gaatgggcga taggtgcggt agtgctgctg
    22441 atcatcagcc tgcttgcgac tctcgaacct ttttgatgga ttacgaaaat gatgaaaaaa
    22501 agtattctgg cgtttctgtt actcaccagt tctgcagcgg cgctggctgc accgcaggtg
    22561 attaccgtca gccgttttga agtgggtaaa gacaaatggg cgtttaatcg cgaagaggtg
    22621 atgctgactt gccgaccggg taatgctttg tatgtcatca acccaagtac cctcgtgcag
    22681 tatcctttaa acgatgtcgc acagaaggaa gttgccagtg ggaagactaa agcccaaccc
    22741 atttcggtga ttcagattga tgatcccaac aatcccggcg aaaaaatgag tctggcaccg
    22801 tttatagaac gagctgaaaa actctgttaa ttacctaaaa tagccttttg atttccaata
    22861 aaaaaaccgc ctcagttctt tcaccagaac gggcggtttt taacatttaa gctgatgacc
    22921 accgcgcttt ttattgacca ttttgcacgc aaactggaaa acctggcgtc gtcatctatt
    22981 cttaaagggc aaggcaacta agcctgcatt aatgccaact tttagcgcac ggctctctcc
    23041 caagagccat ttccctggac cgaatacagg aatcgtgttc ggtctctttt tatctgttaa
    23101 aagccagaag catttccttc gctgacttta tagtcaacca taacacacac tctactgtct
    23161 gagtccagcg ttttttaaca ttcttgttaa gattatgtga tctttagcgc gggaggaaaa
    23221 tattgatgaa acagcctgcg cccgtttatc agagaattgc gggtcatcaa tggcgacata
    23281 tctggctttc tggcgatata cacggttgtc ttgagcagtt gcgccgcaaa ttatggcatt
    23341 gtcgttttga tccgtggcga gatttactta tctcagtggg agacgttatc gatcgtgggc
    23401 cgcaaagttt acgttgtctg cagttactgg aacaacattg ggttcgtgcg gtaagaggca
    23461 atcatgaaca gatggcgatg gatgcgctgg catcccagca gatgtctttg tggttgatga
    23521 atggcggcga ctggtttatt gcgctggcag ataatcaaca gaaacaagcg aaaacggcgc
    23581 tggaaaaatg tcagcatttg ccctttattc ttgaagtaca cagccgcacc ggcaaacatg
    23641 ttattgctca tgccgattat ccagatgatg tttatgaatg gcaaaaggac gttgatttgc
    23701 atcaggtctt gtggagccgc tcgcgattaa gtgaacgcca aaaagggcag ggaattacag
    23761 gtgctgatca tttctggttt ggtcatacac cgttgcgcca tcgcgtggat attggcaacc
    23821 tgcattatat tgataccggc gctgtctttg ggggcgaact gactttcgtg caattgcaat
    23881 aattaaaaat caccatactc ctgtgctggt cgccagaaac catctataaa atcctcaatc
    23941 ggaaaacaac cgccatgacg gatccgttga tcgctcatag aataaagaca ctgctgttcc
    24001 gtgttgtaga catccacaac aatatcttca caaccgccat ccaggtagca aacaaaaagt
    24061 accagcgcga acatttcatc cccgaagtgt ggtgccgtac cgttaagttt aggagagatt
    24121 ttacaacggg ggaataacca ggacaaataa cccgccatgc cgacgggttc tttttggatc
    24181 aggcaagacg cataatccag gcatcagatt gttgatcgta acgggtacgg taaaggacag
    24241 agtgttcgcc atttagcagg gcagggatac tggtttcagc atcccaggca tccagttgca
    24301 tacataaatc ggggtctgat ttacaaggaa tggttaacgt tcgatcaccc ggcgattcac
    24361 cgtgtaattc gccgtcgtcg atttcaaaag cgcctatgcg cacactggtt ttcatcttcg
    24421 tgctctccat ttagctgccg gttgtggtac gaccagtccg atcgcaccat ttttcagcct
    24481 agttgaggcc gccgaatgtg ccagtcaaaa agtgcgcttt ttttgtgcgc tgtcatgcgt
    24541 tactggtaaa aagtttcccg tcccgcacca gttcacgcgg gtagctgttt ttcagtcgcg
    24601 aaccaatccg tttggctaaa ccaatcggct gatgctggaa agtaaccaac acgtcatccg
    24661 ccactggcgc ggcttgcggg taaacatcgc gcccgcgata ccactcctcc gcttcttgcg
    24721 gtgtcagttc aaaagcgttc tcgttgtcgg gggaggcaag ggcaataacc gcttcatgct
    24781 gccagcgata acctttgttg tgcgtttcgg caagtttaat ccccaggcga gaaaatcgga
    24841 ctttaccgat cagggcttca atgcctaccg gaaacaacca cacttctttg tcgcgctgcc
    24901 agagtcgcag gtttccatcc cagtttaagc caacacttgc agccgcctga cgaatttgtg
    24961 cggcttcgcg atctttcacc gggctgaacg ggaaattgcc cactttgtat tttggggtgg
    25021 gtaaggcggg aatggcctga gttttacgca gacgagcaac gaagaagcct tcgcagtcgt
    25081 aaatttgtgg gaaaacatgc aaaaagcctt cttcggtcag cgctttgttt gcaccaggga
    25141 agagatcgcc aagtggtaaa aactctactg cgtcggggta agtctcttta agccacaggc
    25201 aaacggattc gttttcttcc cggtttaagg tacaggtcga gtaaaccagt gtaccgccag
    25261 agcgtaatgc atgaaaggca ctgtcgatca gttcccgttg ggtagctgcg atttcctgat
    25321 tgctttctgg tgaccaattt tttagcgcat cgggatcttt acgcaccacg ccttcgccgg
    25381 agcagggagc gtccagcaaa atggcatcga acatttctgg caatgccgca ccaaacacgc
    25441 ggccatcaaa atgtgtgagc gcaacattac tgataccaca gcggctgata ttggcatgta
    25501 acacttttac ccgactggcg gaaaactcat tggcaaggat tgccccttcg ttattcatcc
    25561 gcgcggcaat ttgcgtcgtt ttggagcctg gcgcagcagc gacatccatc acccgctgtg
    25621 gtgcattacc gtcagcaaac aaggcggcga cgggcaacat tgaactggct tcctgaatat
    25681 aaaacaggcc acttaaatgc tcggcggtac tacccaatgg caatgcatct tcatcgtcgc
    25741 gttcaatcca gaaaccttct tcacaccacg gaattggcgt aagcgtccag ccataaggag
    25801 cggttaatcg caggaaatca gcaacggagg ttttcagcgt attaacgcga atgctgcggc
    25861 gcaacgggcg ctgacaggcg gcaagaaaat catcaaaaga gagcgtcgaa ggcattgctt
    25921 cgcgcatttg cgtcagaaag gcgtccggga aataaacggt gtgttgggcc acgagcatat
    25981 accactggca aaaaaaacag gcgcgcagtt tagcataaac gctccggcgc aggcacgccg
    26041 gagcagtggg cattatttgg gaagcgcagt tccccattca cgccactctt tcggttcact
    26101 ttcctgcaac aggaagtgct tgccttcctg ggctttcggt gccagtggcg tacccggagg
    26161 cgtggcgaag gcgatgccgc cacggataaa ctggttaaag gtgccggttt tcactacgcc
    26221 gcccgtcaga ccaaagtcca gactgtaacc cgatgccaac cagaagacgg aattgttacg
    26281 caccaggtgt tgatagcgtt tactgatgcg catcgcaatc atcacgcgat ctgacaatgt
    26341 ccccagcgtc attccggtaa ccgtaccgac ttccagacca cggaacagca caggcgtacc
    26401 gatgcctaac gaacaggctt ccggcgcttc aacaataatg cttaaaccat ccaggtaacg
    26461 cgaatcagta atggtggcct cttgtaattc aaagtcgcga cgaggattgc cacggcctgg
    26521 ttcgacgttg atatacggct ggaggatagt atcaagatgc tcaacgccag ctgccgaaat
    26581 ttgcggtgtg accactgaga agcgcgtacc accgcgagca aaggtctgga catattccgg
    26641 atagagcacc gcctttgcct gtacttcatt gcgcgcggta atcagatcca gcgtctggat
    26701 ttgcccgata tcaataccga gatagcgaat tggcatcccg accgccagtt ttccggcatc
    26761 gaaagcatga agcgtaatct gcccgccaac cgcacgggcc gctgtttcgg aagcatacag
    26821 aatacgctta tcgcctttac gctgactggc gctggcaccg ctgaggttat cgaagctaat
    26881 ggctcccttt aatgctctgg agagtgggga tgcctgtacg gtcaggccac taccattcag
    26941 ctgaactttc gccccgcctt ctgcccagaa cacgctattg ttggtcagaa ggttgcgata
    27001 ctccggctta atatgcagat cgatatcaaa cgcgttagct cgcggacgca cggtaataac
    27061 ttcaccaact tcaaatttac ggtacagcac taccgatcct gcctgcacat ccggcagcgt
    27121 ctctgcactc aaactcacgg ttgtggtggg taaatcgcta aggctgttct ccagcgcttt
    27181 ttccagattg gcatacagtg gatagctggc tttcatctca cctttatcgc ccggcagaat
    27241 acgtatcccg ccattgatcc attctgaggc gctggcaccg agaaactcaa cgccatccag
    27301 ccccaccttc acatcgacac ggctgttgac gacaaatttg ctatcgcctt ttaccagttc
    27361 gcgatgctga ggctcaatgg cgacggtaaa ggtaacgcct ttgctggtga gtttacgatc
    27421 aataacctgg cctacctgca cgccgtgaag aatgagcggc tgaccagcat caataccgta
    27481 actttccggt gctgtcaggg tcagtgtcag aacatcaggt tcatgcagca gtgctttttc
    27541 gcctggcaca acaacgaact ctttgcgtgg ctcgccatcg ccaggcacca actcaaaggt
    27601 ttttccggtc agcagggcgc tgagattggc atcgctaagg gataatttcg ggttgcgtaa
    27661 ttcgatgcgg gtattttccc gtaacagcgt aacgacgctg ggatcaacgg tcatttctcc
    27721 ggtgacttta ccaccaggat ttaaatccag tttagtcagc tgtccgactt ccagcccctg
    27781 atacattaac ggcgtcgagt cggcggttaa tccggcccca ctcggcagtt ccagttttat
    27841 tattacgcca cgctggctgt gggccagatc ttcatacaga ccaaaggtat cttccgcctc
    27901 ggcaggtttc gactcttctg gtgaatcgaa ggcaatcgca ccgttaacca gtgccgccag
    27961 actttccagt ttcaccttcg cgccactgat actgacgttg gcatcaacgc ctgaaacgtt
    28021 ccagaaacgg ctaccttttt tcaccaggtc ggtaaaacgc cgctcgatca ggacatcaat
    28081 caccacgcct tgtttattgg gattgatggc atagtcgtag acttttccca ccgggatctt
    28141 gcggaaatag accaatgaac cgctgttcag cgaaccgaga tcgggagctt gcaggtggat
    28201 catcagatcg ccgttatcca gccgatactt cggttgggta tcgagcgcga caaagtgatc
    28261 ctgctcttta cctttacctg gcatcatgcc gatatagtta ccaccgacga gggcgtccag
    28321 cccggagaca cctgccaacg atgcttttgg cgtcaccagc cagaactgag tctcttcgcg
    28381 cagcgcatct ttcatatcgg acttgatgct gaccttgact tcaatcttac gaagatcgtc
    28441 gctgaggctg atatcctgca ctgttccgac ttcaacgccc tgataacgaa caggcgtacg
    28501 gcccgggaca ataccatccg ccgacataaa gtcgatggtg acggtattac cccggtcctg
    28561 ataactgtcc caaatcagcc aaccggcaat cattagcgcg atgaacggca gcagccagaa
    28621 aggtgagata cggcgtttat ttttaatctg cgcttcagtc gtcgaagcgg gcgtttcctg
    28681 actcatgtgc atcccaaagt aagcggctgt ccagccattc cacagcaaga atagtcaaaa
    28741 ttaccgctgc gccgaaataa aacgcagccg gtcccatagt aaaagcgagg atctgatcgc
    28801 gattaatcag cgacatggtt aaagatatga caaacaggtc gagcatcgac cagcgaccaa
    28861 tccaggtcac catccgcagt aacagaatgc gtgtgcgtaa accttgctgg catttaaaat
    28921 gaatgctcaa cagtaaagtg aacatgacga tcactttagt aaacggtacc agaatactgg
    28981 cgataaacac gattcctgca accgcaatgt tgctacttgc cagcgacata attccggaaa
    29041 gaattgtatc ttcctgccgt cctccgttca gataaatgat agaaataggc aacaggttgg
    29101 caggtaacaa taaaacgatt gacgctaaca gcgccgccca gcatttttgc agactatgac
    29161 ggcgacgcag gcgtagcgga atatgacaac gcgggcagcg accacgctga tcaggatagc
    29221 cggtaaaatg gcacccaaga cagacacgaa gtttctcgtc cctacgcgta gcggggcgct
    29281 gcggataaaa tcgctcccac agctcctcga cattaagatg tgacaacgtc accgtcgtta
    29341 aaatcaccaa cgccacaaaa gagaacaagc cgacacccgc ctggatatgg gcataatcct
    29401 gtacctttat agaagcaacg ccaatgccga ccaggtagat atcgagcatt acccactctt
    29461 taagtcgctc aagcatcagc agtaccgggc gtagattcat tcccagtcgg ttaccaaacc
    29521 ataaataagc tatggaggtc accagaatga gcggggcacc aataacgcag aaaaagacca
    29581 tcgaccccgt tatcgcatcg ccctgtttgg tcatttgcca gatgccttgc ataacgttgg
    29641 cgtcaatacg aatgcctaac agccagatat gcaacagcgg ttcgccccag gcaaacggca
    29701 tcagcaacag catggtgaag gccattgccg ccaggcgcgt tagcgaccag tcgcgcccgt
    29761 cacgaatttt tgcctgacag cgcggacaat aggcactttg atgagaattt atctcgggca
    29821 ggctaaacag catgtcacat tgcgggcaac gttggtaatc accacgcggc agttcctcgc
    29881 cgattgccct cactgttatc ttttttgtcg gcgtaatttg tggtgtgtta agagccatga
    29941 cgtgccaaat gtaataagaa ttaacattaa tcttaaccca tgatgggaat gatcttgagc
    30001 attaacgcat ttaatgctta ttttaatagg ctggcagttc caggtaagac aaccaagtga
    30061 ttatataatg aacaaaacag aattttacgc ggatttaaat cgcgacttta acgcgctgat
    30121 ggcgggagaa accagttttc tggcaacgct tgcgaacacc agtgcgttgt tatatgagcg
    30181 tctcactgac gtaaactggg caggttttta tttgcttgag gacgatacac tggtgctcgg
    30241 accatttcag ggcaaaattg cctgtgtccg gatacccgtc gggcgtggcg tgtgcggcac
    30301 tgcggttgcc cgcaatcaag tgcagcgtat cgaggatgtt catgcgtttg acgggcatat
    30361 tgcctgtgat gcggcgagta attctgaaat tgttctgccg ctggtggtga aaaatcagat
    30421 tattggtgtt ctcgacatcg atagtaccgt cttcggtcgc tttacagacg aggacgagca
    30481 aggcttacgt cagcttgtgg cacagcttga aaaagtgctt gcaacgacgg attacaaaaa
    30541 attctttgcg agcgtcgcag gataatcaac ggataacgta gcatttactg atagcgtcat
    30601 tataatgacg cctgttcatg cctgcgcttg ttggctacgt ccgttgtaat caggaaattt
    30661 catggaaaat caacctaagt tgaatagcag taaagaagta atcgcgtttc tggccgaacg
    30721 ttttccccac tgtttcagtg cggaaggtga agcgcgtccg ctgaaaatcg gtatttttca
    30781 ggatttggtc gatcgtgttg ccggggaaat gaacctgagc aaaacgcaat tacgatccgc
    30841 tttacgtctc tacacttcga gctggcgtta cctttacggt gttaaacccg gcgcaacgcg
    30901 tgtcgatctt gacggcaacc catgcggtga gctggacgag caacatgtag agcatgctcg
    30961 caagcagctt gaagaagcga aagcgcgtgt tcaggcacag cgtgctgaac agcaagcgaa
    31021 aaaacgcgaa gctgccgcag ctgctggtga gaaagaagac gcaccgcgtc gcgaacgcaa
    31081 gccacgtccg accacgccac gccgcaaaga aggcgctgaa cgtaaacctc gtgcgcaaaa
    31141 gccggtagag aaagcgccaa aaacagcaaa agcacctcgc gaagaacagc acaccccagt
    31201 ttctgacatt tcagctctga ctgtcggaca agccctgaag gtgaaagcgg gtcaaaacgc
    31261 gatggatgcc accgtattag aaatcaccaa agacggcgtc cgcgtccagc tgaattcggg
    31321 tatgtctttg attgtgcgcg cagaacacct ggtgttctga aacggaggcc gggccaggca
    31381 tgaacatgtt ttttaggctt accgcgttag ctggcctgct tgcaatagca ggccagacct
    31441 tcgctgtaga agatatcacg cgtgctgatc aaattccggt attaaaggaa gagacgcagc
    31501 atgcgacggt gagtgagcgc gtaacgtcgc gcttcacccg ttctcattat cgccagttcg
    31561 acctcgatca ggcattttcg gccaaaatct ttgaccgcta cctgaatctg ctcgattaca
    31621 gccacaacgt gctgctggca agcgatgttg aacagttcgc gaaaaagaaa accgagttag
    31681 gcgatgaact gcgttcaggc aaactcgacg ttttctacga tctctacaat ctggcgcaaa
    31741 agcgtcgttt tgaacgttac caatacgctt tgtcggtact ggaaaagccg atggatttca
    31801 ccggcaacga cacttataac cttgaccgca gcaaagcgcc ctggccgaaa aacgaggctg
    31861 agttgaacgc gctgtgggac agtaaagtca aattcgacga gttaagcctg aagctggcag
    31921 gaaaaacgga taaagaaatt cgtgaaaccc tgactcgccg ctacaaattt gccattcgtc
    31981 gtctggcgca aaccaacagc gaagatgttt tctcgctggc aatgacggcg tttgcgcgtg
    32041 aaatcgaccc gcataccaac tatctttccc cgcgtaatac cgaacagttc aacactgaaa
    32101 tgagtttgtc gctggaaggt attggcgcag tgctgcaaat ggatgatgac tacaccgtta
    32161 tcaattcgat ggtggcaggt ggtccggcag cgaagagtaa agctatcagc gttggtgaca
    32221 aaattgtcgg tgttggtcaa acaggcaagc cgatggttga cgtgattggc tggcgtcttg
    32281 atgatgtggt tgccttaatt aaagggccga agggcagtaa agttcgtctg gaaattttac
    32341 ctgctggtaa agggaccaag acccgcactg taacattgac ccgtgaacgt attcgtctcg
    32401 aagaccgcgc ggttaaaatg tcggtgaaga ccgtcggtaa agagaaagtc ggcgtgctgg
    32461 atattcctgg cttctatgtg ggtttgacag acgatgtcaa agtgcaactg cagaaactgg
    32521 aaaaacagaa tgtcagcagt gtgatcatcg atctgcgtag caatggtggt ggggcgctga
    32581 ccgaagcggt atcgctctca ggtctgttta ttcctgcggg tcctattgtt caggtccgtg
    32641 ataacaacgg taaagttcgt gaagacagcg ataccgacgg acaggttttc tataaaggcc
    32701 cgctggtggt actggttgac cgcttcagtg cttcggcttc agaaatcttt gccgcggcaa
    32761 tgcaggatta cggtcgtgca ctggttgtgg gtgaaccgac gtttggtaaa ggcaccgttc
    32821 agcaataccg ttcattgaac cgtatttacg atcagatgtt acgtcctgaa tggccagcgc
    32881 tgggttctgt gcagtacacg atccagaaat tctatcgcgt taacggcggc agtacgcaac
    32941 gtaaaggcgt aacgccagac atcatcatgc cgacgggtaa tgaagaaacg gaaacgggtg
    33001 agaaattcga agataacgcg ctgccgtggg atagcattga tgccgcgact tatgtgaaat
    33061 caggcgattt aacggccttt gaaccggagc tactgaagga acataatgcg cgtatcgcga
    33121 aagatcctga gttccagaac atcatgaagg atatcgcgcg cttcaacgct atgaaggaca
    33181 agcgcaatat cgtttctctg aattacgctg tgcgtgagaa agagaataat gaagatgatg
    33241 cgacgcgttt ggcgcgtttg aacgaacgct ttaaacgcga aggtaaaccg gagttgaaga
    33301 aactggatga tctaccgaaa gattaccagg agccggatcc ttatctggat gagacggtga
    33361 atatcgcact cgatctggcg aagcttgaaa aagccagacc cgcggaacaa cccgcacccg
    33421 tcaagtaata tcaatcaggc acaagaaatt gtgcctgatt ttttaacagc ggcaagatgc
    33481 cgtaaatcag atgctacaaa atgtaaagtt gtgtctttct ggtgacttac gcactatcca
    33541 gacttgaaaa tagtcgcgta acccatacga tgtgggtatc gcatattgcg ttttgttaaa
    33601 ctgaggtaaa aagaaaatta tgatgcgaat cgcgctcttc ctgctaacga acctggccgt
    33661 aatggtcgtt ttcgggctgg tactgagcct gacagggata cagtcgagca gcgttcaggg
    33721 gctgatgatc atggccttgc tgttcggttt tggtggttcc ttcgtttcgc ttctgatgtc
    33781 caaatggatg gcattacgat ctgttggcgg ggaagtgatc gagcaaccgc gtaacgaaag
    33841 ggaacgttgg ctggtcaata ctgtagcaac ccaggctcgt caggcgggga tcgctatgcc
    33901 gcaagtggct atctaccatg cgccggacat caacgctttt gcaaccggtg cgcgccgtga
    33961 tgcctctctg gttgctgtca gcaccggttt gctgcagaac atgagcccgg atgaagccga
    34021 ggcggtaatt gctcacgaaa tcagccacat cgccaatggt gatatggtca ccatgacgct
    34081 gattcagggc gtggtgaaca ccttcgttat ctttatttcc cgtatcctgg cgcagcttgc
    34141 cgcgggtttt atgggcggaa atcgtgatga aggtgaagag agcaacggca acccgctgat
    34201 ctactttgcg gttgcaacgg ttctggaact ggtgtttggt attctggcga gcattatcac
    34261 catgtggttc tcgcgtcatc gtgaattcca tgctgatgcc ggttcggcaa aactggttgg
    34321 tcgcgagaaa atgattgccg cgctgcagcg cctgaaaacc agctatgaac cgcaagaagc
    34381 aaccagcatg atggctttct gcattaacgg taagtcgaaa tcgctcagtg agttgtttat
    34441 gactcacccg ccgctggata aacgtattga agctctgcgt acgggtgaat acctgaagta
    34501 atatgaagta aatactaaaa agcgcgtcct gctcgacgcg ctttttttat gccctggatc
    34561 ttggctgagt gatacgtaac ccactgatac aggcagcaat cactgccaga atagccgcag
    34621 ccatcagcga gacgtgtgta ccattatctc caaactgatt tagcatcagc gccaccagcg
    34681 ccgcgccgct actctgaccc agtagacgag ccgttcctaa catgccactg gctccaccgc
    34741 tacgttcgcg aggcgcggag gtaataatgg tgtggttatt gggtgactgg aacaagccaa
    34801 atccagcgcc acataagatc atcggccaga taatattgat atccgcaggt gacgcgggca
    34861 gcagaaccag ggaaaaaagc cccgcagcca tgatgaataa tcctaaagca cccagcaatc
    34921 ctgcatgtac gcgttcaatc aaatagcctg ccaacggagc catcaccatc gttgctaacg
    34981 gccacggtgt cagaagtaaa cctgtttcga cttcactacg cccgagcacg gtttgcaggt
    35041 aaaagggcag ggaaaccatt gccagcattt gtgcgcagaa agagcaaaca gatgtgcaaa
    35101 tagaaagtga aaacagcggg atacgcagta aatccaccgg tagcagcggt actggaagag
    35161 aaagctggcg gcgaataaag aaaataccaa cgacaaccat tacaaccagt tccgcagcaa
    35221 ttaacgtcag cgattgcccc tgagcgaaac cactcaacgc agtgataagc aggccgaagg
    35281 ttaacgcgtt catcacggcg ctgggcaggt cgaaacgggg tttactggcg cgagaaccat
    35341 tgggtggcag aaaacgcatc gccagaagca gggcgataat acccaacggt acgttgatta
    35401 aaaataacca tttccaggat gcgatggaga ggattgctgc agcaattgtc ggcccggcag
    35461 cagaagagac ggcaacaata aacgagttta tgcccatccc tctacccaga aaacgttgtg
    35521 gatagatcag gcggataagg gcggtattaa cgctcatcaa cgctgcaccg ccgaaacctt
    35581 gtatgacacg cgcaagggtg agtatttgca gcgaatcaga aagggcgcag aacagtgaag
    35641 acaacagaaa aacgaccaga ccgcatttat aaatacgtcg atagccaaac atatcaccca
    35701 gaaacgaaaa cgagagcagg gagatgacaa tggcgatttg ataggcgttc actacccaga
    35761 tggaactggc tggcgtggca tgaaggtccg tggcgattgt tggcagggcg acgttggcga
    35821 ttgcgccgtc aaggacagcc atcgaaatac caatcacaat ggttaatatc gcaccgtatc
    35881 gctggggcaa tggcaggccg tcggcctgaa cttttggcat aggaatttta aatctttggt
    35941 gaataatcaa caggggtatg attctagcac tgatattcac ggtggatgtc gcagaattgt
    36001 aactaaccgg caaaagatgg attgcgacca ccctggtgcg aaattataat aaaaaacagt
    36061 tctgattttt ataaaacact cgcaatgagg tgataaatgg ctaacgcaga tctggataaa
    36121 cagcctgatt ctgtatcttc cgtgctaaaa gtttttggca ttttgcaggc gctgggtgaa
    36181 gagcgcgaaa tagggataac cgagctgtcg cagcgcgtca tgatgtcaaa aagcaccgtt
    36241 tatcgctttt tacagaccat gaaaacctta ggttatgtgg cgcaggaagg ggagtcggag
    36301 aaatattccc tgaccctgaa attgtttgaa ctgggcgctc gcgcgttaca aaacgtcgat
    36361 ttaattcgta gcgcagatat ccagatgcgt gagatctccc gcctgaccaa agaaactatc
    36421 cacctcggcg cactggacga agacagtatt gtttacattc acaaaattga ctctatgtac
    36481 aatttgcgca tgtattcacg gattgggcgt cgtaatccac tgtacagcac cgcgattggt
    36541 aaggtactgc tggcatggcg cgatcgcgat gaagtgaagc aaattcttga gggcgtggag
    36601 tataaacgca gtaccgagcg gaccatcacc agtacagaag cgttattacc ggttctggac
    36661 caggtgcgcg agcaggggta tggcgaagat aacgaagagc aggaagaagg gctgcgatgc
    36721 attgcggttc cggtatttga tcgctttggc gtggtcattg ccggtttgag catctccttc
    36781 ccgacgttgc gtttctctga agagcgttta caggaatatg tcgcaatgtt gcataccgca
    36841 gcgcgcaaaa tttctgccca aatgggttat cacgactatc cgttctgatg agagtaagaa
    36901 cctgtcggaa tatcaaacag acaggttctt tatttagcat gagaaaaata aagttgaagg
    36961 tggcgttaga ttaaacgcag ttgatataag aatattttac tcatgagtga gaatctggtt
    37021 atttattaca cttaaccatt atcgaccacg atattgcttt tgcgtaacag cgggcaatct
    37081 gttatcccca aaaaaccact tttagtgtgc aagtattgta ccgtgctggt gcctctggca
    37141 gtcagatagg tacattgcaa acctaattct gcggcattct ctttgcttcc aatcaaaacg
    37201 ccatatccgc tgagtaataa tcctatccat accagtgcta tcagcataac tgtgcgaatg
    37261 atgaatcgca ttacaacctc ttctcttttt tatgttcgct taatcgtagc ggcaatatgc
    37321 gctgaagcaa gcgactcatt ccgaaaaagc acgaatatcg acatagttag acgctgttta
    37381 actaacgcat ggtagtttaa tgacataagg taggtgaaac ggagattgga gtgaaaaagt
    37441 ttcgatgggt cgttttggtt gtcgtggtgt tggcttgctt gctgctttgg gcgcaggtat
    37501 tcaacatgat gtgcgatcag gatgtacaat ttttcagcgg aatttgtgcc attaaccagt
    37561 ttatcccgtg gtgatatcat tttttcagca ctagattttc ttcctctcca gcagtggtag
    37621 aatagcgcct ctactcagag gtggtgaaat gaacgaagtt gtaaattcag gcgtgatgaa
    37681 cattgcgtct ttggttgtat cggtggtggt tcttcttatc gggctcatct tgtggttttt
    37741 tattaatcgt gccagttctc ggactaacga acagattgaa ctgcttgagg cgttgctgga
    37801 tcagcaaaaa cgtcaaaatg cactgttacg tcgtttgtgc gaagcaaacg aaccagagaa
    37861 agcagataaa aagactattg agagtcaaaa atcggttgaa gacgaagata ttattcgcct
    37921 ggtcgccgaa cgataatcct tcctcaatct tgagtaaccc cgtcgttgaa gggttactca
    37981 attcatcctg attatacgca ctgtcatggt gcaactgctt caaattaaa
//
LOCUS       DAZFAB010000036        37494 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZFAB010000036 DAZFAB010000000
VERSION     DAZFAB010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37494
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.036"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            243..1616
                     /gene="aroP"
                     /locus_tag="ACHJWA_002132"
     CDS             243..1616
                     /gene="aroP"
                     /locus_tag="ACHJWA_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFO4308729.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVTVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAVVLVIMLMTPGMAISVYLIPVWLVVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(1659..2513)
                     /gene="ampE"
                     /locus_tag="ACHJWA_002133"
     CDS             complement(1659..2513)
                     /gene="ampE"
                     /locus_tag="ACHJWA_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFO4308730.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFRTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2510..3061)
                     /gene="ampD"
                     /locus_tag="ACHJWA_002134"
     CDS             complement(2510..3061)
                     /gene="ampD"
                     /locus_tag="ACHJWA_002134"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFO4308731.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            3149..4042
                     /gene="nadC"
                     /locus_tag="ACHJWA_002135"
     CDS             3149..4042
                     /gene="nadC"
                     /locus_tag="ACHJWA_002135"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFO4308732.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4245..4685
                     /gene="ppdD"
                     /locus_tag="ACHJWA_002136"
     CDS             4245..4685
                     /gene="ppdD"
                     /locus_tag="ACHJWA_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFO4308733.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            4695..6080
                     /gene="gspE"
                     /locus_tag="ACHJWA_002137"
     CDS             4695..6080
                     /gene="gspE"
                     /locus_tag="ACHJWA_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFO4308734.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLAGFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQKLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPVNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            6070..7272
                     /gene="hofC"
                     /locus_tag="ACHJWA_002138"
     CDS             6070..7272
                     /gene="hofC"
                     /locus_tag="ACHJWA_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFO4308735.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQRAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGIMLTIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNIAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(7307..8350)
                     /gene="guaC"
                     /locus_tag="ACHJWA_002139"
     CDS             complement(7307..8350)
                     /gene="guaC"
                     /locus_tag="ACHJWA_002139"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFO4308736.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIIS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(8506..8550)
                     /locus_tag="ACHJWA_002140"
     CDS             complement(8506..8550)
                     /locus_tag="ACHJWA_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4308737.1"
                     /translation="MIIKTLKMSARKRN"
     gene            8575..9195
                     /gene="coaE"
                     /locus_tag="ACHJWA_002141"
     CDS             8575..9195
                     /gene="coaE"
                     /locus_tag="ACHJWA_002141"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFO4308738.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9195..9938
                     /gene="zapD"
                     /locus_tag="ACHJWA_002142"
     CDS             9195..9938
                     /gene="zapD"
                     /locus_tag="ACHJWA_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFO4308739.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDCQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            9948..10145
                     /gene="yacG"
                     /locus_tag="ACHJWA_002143"
     CDS             9948..10145
                     /gene="yacG"
                     /locus_tag="ACHJWA_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFO4308740.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(10236..10634)
                     /gene="mutT"
                     /locus_tag="ACHJWA_002144"
     CDS             complement(10236..10634)
                     /gene="mutT"
                     /locus_tag="ACHJWA_002144"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFO4308741.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     KWMSLVGLNADDFPPANEPVIAKLKRVYVG"
     gene            complement(10694..13399)
                     /gene="secA"
                     /locus_tag="ACHJWA_002145"
     CDS             complement(10694..13399)
                     /gene="secA"
                     /locus_tag="ACHJWA_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFO4308742.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLAAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(13461..13973)
                     /gene="secM"
                     /locus_tag="ACHJWA_002146"
     CDS             complement(13461..13973)
                     /gene="secM"
                     /locus_tag="ACHJWA_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFO4308743.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLS"
     gene            complement(14204..15121)
                     /gene="lpxC"
                     /locus_tag="ACHJWA_002147"
     CDS             complement(14204..15121)
                     /gene="lpxC"
                     /locus_tag="ACHJWA_002147"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFO4308744.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(15222..16373)
                     /gene="ftsZ"
                     /locus_tag="ACHJWA_002148"
     CDS             complement(15222..16373)
                     /gene="ftsZ"
                     /locus_tag="ACHJWA_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFO4308745.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(16434..17696)
                     /gene="ftsA"
                     /locus_tag="ACHJWA_002149"
     CDS             complement(16434..17696)
                     /gene="ftsA"
                     /locus_tag="ACHJWA_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFO4308746.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(17693..18523)
                     /gene="ftsQ"
                     /locus_tag="ACHJWA_002150"
     CDS             complement(17693..18523)
                     /gene="ftsQ"
                     /locus_tag="ACHJWA_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFO4308747.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(18525..19445)
                     /gene="ddlB"
                     /locus_tag="ACHJWA_002151"
     CDS             complement(18525..19445)
                     /gene="ddlB"
                     /locus_tag="ACHJWA_002151"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO4308748.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRVEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSVRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(19438..20913)
                     /gene="murC"
                     /locus_tag="ACHJWA_002152"
     CDS             complement(19438..20913)
                     /gene="murC"
                     /locus_tag="ACHJWA_002152"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFO4308749.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(20967..22034)
                     /gene="murG"
                     /locus_tag="ACHJWA_002153"
     CDS             complement(20967..22034)
                     /gene="murG"
                     /locus_tag="ACHJWA_002153"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFO4308750.1"
                     /translation="MSAQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(22031..23275)
                     /gene="ftsW"
                     /locus_tag="ACHJWA_002154"
     CDS             complement(22031..23275)
                     /gene="ftsW"
                     /locus_tag="ACHJWA_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFO4308751.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(23275..24591)
                     /gene="murD"
                     /locus_tag="ACHJWA_002155"
     CDS             complement(23275..24591)
                     /gene="murD"
                     /locus_tag="ACHJWA_002155"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFO4308752.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGVQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(24594..25676)
                     /gene="mraY"
                     /locus_tag="ACHJWA_002156"
     CDS             complement(24594..25676)
                     /gene="mraY"
                     /locus_tag="ACHJWA_002156"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFO4308753.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLVGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(25670..27028)
                     /gene="murF"
                     /locus_tag="ACHJWA_002157"
     CDS             complement(25670..27028)
                     /gene="murF"
                     /locus_tag="ACHJWA_002157"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFO4308754.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIVWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFAEKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(27025..28512)
                     /gene="murE"
                     /locus_tag="ACHJWA_002158"
     CDS             complement(27025..28512)
                     /gene="murE"
                     /locus_tag="ACHJWA_002158"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFO4308755.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKGEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQDATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEDRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(28499..30265)
                     /gene="ftsI"
                     /locus_tag="ACHJWA_002159"
     CDS             complement(28499..30265)
                     /gene="ftsI"
                     /locus_tag="ACHJWA_002159"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFO4308756.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(30281..30646)
                     /gene="ftsL"
                     /locus_tag="ACHJWA_002160"
     CDS             complement(30281..30646)
                     /gene="ftsL"
                     /locus_tag="ACHJWA_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFO4308757.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(30643..31584)
                     /gene="rsmH"
                     /locus_tag="ACHJWA_002161"
     CDS             complement(30643..31584)
                     /gene="rsmH"
                     /locus_tag="ACHJWA_002161"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFO4308758.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(31586..32044)
                     /gene="mraZ"
                     /locus_tag="ACHJWA_002162"
     CDS             complement(31586..32044)
                     /gene="mraZ"
                     /locus_tag="ACHJWA_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFO4308759.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(32646..33650)
                     /gene="cra"
                     /locus_tag="ACHJWA_002163"
     CDS             complement(32646..33650)
                     /gene="cra"
                     /locus_tag="ACHJWA_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFO4308760.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(33732..33818)
                     /locus_tag="ACHJWA_002164"
     CDS             complement(33732..33818)
                     /locus_tag="ACHJWA_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFO4308761.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(33830..34321)
                     /gene="ilvN"
                     /locus_tag="ACHJWA_002165"
     CDS             complement(33830..34321)
                     /gene="ilvN"
                     /locus_tag="ACHJWA_002165"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO4308762.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLYKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(34324..36048)
                     /gene="ilvI"
                     /locus_tag="ACHJWA_002166"
     CDS             complement(34324..36048)
                     /gene="ilvI"
                     /locus_tag="ACHJWA_002166"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFO4308763.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGIG
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGGAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPQELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            complement(36366..37310)
                     /gene="leuO"
                     /locus_tag="ACHJWA_002167"
     CDS             complement(36366..37310)
                     /gene="leuO"
                     /locus_tag="ACHJWA_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308107.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFO4308764.1"
                     /translation="MPEVQTDHPETAEFSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKLNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT         9069 a        10442 c         9143 g         8840 t
ORIGIN      
        1 tctgcatatt gagaggggtt gaggctgagc tttacaaacg gtttcttttt aagcaactca
       61 tcttcaacca tgcataaagc gggtgcattc gctgccgcat accattattc ttgatctgac
      121 ggaagtcttt ttgtaactat tcaaacttct ttgatgtaaa caaattaata caacaaacgg
      181 aattgcaaac ttacacacgc atcactgcgt agatcaaaaa aacaatcacc gcacgaggtt
      241 tcatgatgga aggtcaacag cacggcgagc agctaaagcg cggccttaaa aaccgccata
      301 ttcagcttat cgcgctgggt ggcgcgatag ggacagggtt attcctgggt agcgcctccg
      361 taatacagtc cgcagggcca gggattatcc tgggttacgc cattgctggt tttatcgcct
      421 ttctgatcat gcgtcagctg ggtgaaatgg tggtcgaaga acctgtcgca ggctccttta
      481 gccactttgc ttataaatac tggggcagct ttgctggctt cgcttctggc tggaactact
      541 gggtactgta cgttttagtt gccatggcag agctgactgc tgtgggtaaa tacattcagt
      601 tctggtatcc ggaaatccca acctgggttt ctgccgccgt gttctttgtg gtgattaacg
      661 ccatcaacct gaccaacgta acagtgtttg gtgagatgga gttctggttt gccattatca
      721 aagttattgc ggtagtagcg atgatcatct tcggcggctg gctgctgttc agtggtaacg
      781 gcggtccgca ggcaagcgtt agcaacctgt gggatcaggg cggtttcctg ccgcacggct
      841 tcaccgggct ggtgatgatg atggcgatta tcatgttctc gttcggtggt ctggaactgg
      901 tggggatcac cgcagcagaa gctgataacc cggagcaaag tatcccgaaa gcaactaacc
      961 aggttatcta ccgcatcctg attttctata ttggttcgtt agccgttctg ctctcactga
     1021 tgccgtggac ccgcgttacc gccgatacca gtccgtttgt gctgatcttc cacgagttag
     1081 gcgatacctt tgtggcgaat gcgctgaaca tcgtggtact gactgcggcg ctctccgtgt
     1141 acaacagctg cgtatattgc aacagccgta tgctgtttgg tctggcacaa cagggtaacg
     1201 cgccaaaagc gctggcgtct gtcgataaac gcggcgtacc ggttaacacc attctggtgt
     1261 ctgcgctggt tacagcattg tgcgtattga ttaactatct tgctccggaa tccgcatttg
     1321 gcctgttaat ggcactggtg gtatccgcac tggtgatcaa ctgggcgatg atcagtctgg
     1381 cgcatatgaa gttccgtcgc gccaagcagg aacaaggcgt ggtaactcgc ttccctgctc
     1441 tgctttatcc gctgggtaac tggatctgcc tgctgtttat ggcggtggta ctggtgatta
     1501 tgctgatgac cccaggaatg gcgatttcgg tatacctgat cccggtatgg ctggtggtgt
     1561 taggtatcgg ctatctgttt aaagagaaaa ccgcaaaagc cgtaaaagca cattaatctc
     1621 tctacgccct cacccgtaca gggtgagggc aataatcttt acaccaacgc cccgtaaatc
     1681 gtcagtagcg caatcaccac cacgaccacg aacgaggttt tcttcgccat tgaaaccgct
     1741 gccttcggcg tctccacctt atcgacatgc ggttcacgcg ccagagagaa ctgcgccaga
     1801 cgcgttaata cctgatattg cgaagtacgg aaatcaccca gcgaagcaaa ccaggccggt
     1861 aacgctttct caccatgacc gatcaaggca tataccacac ctgcaagacg aaccggcacc
     1921 caatccagta catgaagcac tgcatcaatg ccggactgta aacgatgatg cggcgtctgg
     1981 tatcgcgcca gccagtattg ccatgcacgc aaaaacgcat accccatcag cgtaacgggt
     2041 ccccaggttc cccccacaat cagccagaac agcggtgcaa gataaaaacg aaagttaatc
     2101 cacagcaatg cattttgcag ctcacgcaaa tactcacgtt cgtcgcagcc tgccgggacg
     2161 ccgtgaatca tggtgagttc gccagccatc gtggcacggg catggctatc attacgtgaa
     2221 gcagctgtca gataagcatg ataatgaaga cgaactttac ctgcgccaat acacagcaaa
     2281 ccaatcagca gccacaccag cagcgtggga acgttgaaca atactccctg caatgcgcgt
     2341 aacagtaaaa aagtcacgcc catcgcaata atggtcatgc ctaacgtgcg cccgagagaa
     2401 aaatgtttca cccgccgaaa gaacgcttca agacgatgat caagctgcca gtgctcgccc
     2461 aacttaaaca ggcgctcgaa aattaacacc agtaaggttg taaatagcgt catgttgtct
     2521 ccttgctgac cagcgcacga aaccgtgccc aatcaaatgc aggaccggga tcggttttcc
     2581 gatccggcgc aatatcacaa tggcccgtca tgttattagc gatatccgga tagcaatcaa
     2641 tcagtgcccg cgtaaccgcc gcaagctgtt gatactgcgc atcggtatac gccagcgtat
     2701 cggtgccttc aagctcaatc ccaatagaaa aatcattgca gcgttcgcgc ccctgatact
     2761 gagagactcc cgcatgccat gcacgtttat cgaaaggaac atactggact atttcaccat
     2821 cacggcgaat caaacagtga gcggagacgc gcaaatgggc gatctcagca aagaaaggat
     2881 gtgcctgcgg atcaatagtt ccagtgaata atgcgtcgat ccacggaccg ccaaactcgc
     2941 ctggcggcag gctaatattg tgcaccacca gcagggtggg tgtttcgtca tccgggcggc
     3001 aatcgtaatg tggtgaggga acgcggcgcg cgccaaccag ccacccctgt tctaacaaca
     3061 tgcaggatct ccttatatgt ggtgctaata cccggttcag agtagcatgt ttctacctta
     3121 tgattcgtta gctatctgga gttttaacat gccgcctcgc cgctataacc ctgacacccg
     3181 acgtgacgag ctgctggaac gcattaatct cgatatcccc ggcgcggtgg cccaggcgct
     3241 gcgggaagat ttaggcggaa cagtcgatgc caacaatgac attacggcaa aacttttacc
     3301 ggaaaattct cgctctcatg ccacggtgat cacccgcgag aatggcgtat tttgcggcaa
     3361 acgctgggtt gaagaggtgt ttattcaact ggcaggcgac gatgtcacca taatctggca
     3421 tgtggatgac ggcgatgtca tcaacgccaa tcaacccttg ttcgaacttg aaggcccatc
     3481 ccgcgtgctg ttaacgggcg aacgcactgc gcttaatttt gtgcaaaccc tttcaggagt
     3541 tgccagtaag gtacgccact atgtcgaatt actggaaggc accaacacgc agttgttgga
     3601 tacgcgtaaa accttacccg gtctgcgttc agcgctgaaa tacgcggtac tttgcggcgg
     3661 cggagcgaat caccgtctgg ggctttctga tgccttcctg atcaaagaaa accatattat
     3721 tgcctccggc tcagtgcgcc aggcggtcga aaaagcgtcc tggctgcacc cggatgcgcc
     3781 agtagaagtc gaagtagaga atctggaaga acttgatgaa gccctgaaag caggagccga
     3841 tatcatcatg ctggataact tcgaaacaga acagatgcgc gaagccgtca aacgcaccaa
     3901 cggcaaggcg ctactggaag tgtctggcaa cgtcactgac aaaacactgc gcgaatttgc
     3961 cgaaacgggc gtggacttta tctccgtcgg tgcgctaact aagcacgtac aggcactcga
     4021 cctttcaatg cgttttcgct agttgctgac agatttatcc gctccatagt gggcggataa
     4081 tctccccttc cccaaccttc ttcgtaacgc ctcgcaaatt tcttgtcttt cgctgcaacg
     4141 aaataaaaaa cctcggaagc agctttcccg tttcgttttt tgcccctcgc ctgtgctgat
     4201 ggcgtttgcc aaagtagcac caaccaaatc aaggagcgaa acagatggac aagcaacgcg
     4261 gttttacact tatcgaactg atggtggtta ttggcatcat tgccatttta agcgccattg
     4321 gtattcccgc ttatcaaaac tacctgcgca aagccgcact caccgacatg ctacaaacct
     4381 ttgtgcctta ccgtaccgcc gtagagttgt gcgcgctgga acatggtgga ttagatacct
     4441 gcgacggtgg cagcaatggc attccctcgc ctaccaccac ccgctatgtt tcagccatga
     4501 gtgtggcaaa gggcgtggtg tcgctgaccg ggcaagaaag tctcaatggg ctaagcgtcg
     4561 tcatgacgcc aggttgggat aacgcaaacg gcgtcaccgg ctgggcgcgc aactgcaata
     4621 ttcaaagtga cagcgcattg cagcaagcct gcgaagatgt cttccgcttt gatgacgcca
     4681 actaaggagc ggcaatgaat attccacagc tcacggccct gtgcctgcgt tatcagggag
     4741 tcttgctgga tgccagcgaa gaagtggttc atgttgcggt ggtcgatgcc ccctcacatg
     4801 agttgctgga cgcattgcat ttcgctacca ccaaacgtat tgagatcacc tgctggacgc
     4861 gccaacaaat ggaaggtcac gccagtcgca cacaacagac attgcccgta gctgttcagg
     4921 agaagcatca gcccaaagca gagttgctga ctcgaacgtt acaatctgcg ctggaacaac
     4981 gcgcgtctga tattcatatc gaaccagcgg acaatgccta ccgcatccgc ttgcgtatcg
     5041 acggcgtatt gcatccttta ccggatgttt caccggatgc cggagtcgca ttaaccgcca
     5101 gattaaaagt gctgggaaac ctggatattg cggaacatcg cctgccgcag gacgggcaat
     5161 tcactgtcga actggcagga aacgccgtct catttcgtat tgcgacctta ccatgtcggg
     5221 gtggtgaaaa ggtggtatta aggttgttac agcaggtgag ccaggcactg gatgttaaca
     5281 cgctgggaat gcagccgtta caactggcgg gctttgctca tgccttgcaa caaccacagg
     5341 gactggtgct ggtaactggc cctaccggca gcggcaaaac ggtcacgctt tatagtgccc
     5401 tgcaaaagct gaataccgct gacattaata tttgtagcgt cgaagatccg gttgagatcc
     5461 ccatagccgg actaaaccag acgcaaatcc atccgcgtgc cggactcacc tttcagggcg
     5521 ttttgcgtgc gttattgcgc caggatcctg acgtcatcat gatcggagag atccgcgatg
     5581 gcgaaacggc agaaattgcc attaaagccg cgcaaaccgg tcacctggtg ttgtctaccc
     5641 tacacactaa ttccacctgc gaaacgctgg tacgtttaca gcaaatgggg gtcgcccgct
     5701 ggatgctatc atcggcgctt acgctggtaa tagcccagcg tctggtacgc aaactttgcc
     5761 cacattgtcg ccggcagcaa ggggagccca tccacattcc agtcaatgta tggccgtcgc
     5821 cgctgcccca ctggcaagca cccggttgtg tacattgcta ccacggtttt tatggtcgca
     5881 ctgccttatt tgaagttctg cccataacac cggtcatacg tcagcttatt tccgctaata
     5941 ccgacgttga atcgctggaa acgcacgccc gacaggcggg tatgcgaacg ctttttgaaa
     6001 acggctgcct ggccgtggag caaggcttaa ccacctttga agagttaatc cgcgtattgg
     6061 ggatgccgca tggcgagtaa gcaactctgg cgctggcatg gcataaccgg cgacggcaat
     6121 gcgcaagatg ggatgctatg ggcagagagc cgtactttac tgcttatggc actacagcaa
     6181 cagatggtta ccccactaag cctgaagcga attgctatca attctgcgca gtggcgagga
     6241 gataaaagcg cggaagtcat tcatcaactg gcgacgctac ttaaagccgg gttaacgctt
     6301 tctgaagggc tggcactgct ggcggaacag catcccagta agcaatggca agcgttgctg
     6361 caatctctgg cacacgatct cgaacagggc attgcctttt ccaatgcctt attaccctgg
     6421 tcagaggtat ttccgccgct ctatcaggcg atgatccgca cgggtgaact gaccggtaag
     6481 ctggatgaat gctgctttga actggcgcgt cagcaaagag cccagcgtca gttgaccgac
     6541 aaagtaaaat cagcgttacg ttatcccatc atcattttag cgatggcaat catggtggtt
     6601 gtggcaatgc tgcattttgt tctgccggag tttgccgcta tctataagac cttcaacacc
     6661 ccactaccgg cactaacgca ggggatcatg acgctggcag actttagtgg cgaatggggc
     6721 tggctgctgg tgttgttcgg cattatgctg acgatagcca ataagttgct gatgcgccgc
     6781 ccgacctggc ttatcgcgcg gcagaaattg ctgttacgca tcccgattat ggggtcactg
     6841 atgcggggac aaaaactcac gcagatcttt acgattctgg cgctgactca aagtgcaggc
     6901 attacttttt tacagggcgt agagagcgtc agagaaacaa tgcgctgccc gtactgggtg
     6961 caacttctga cacaaatcca gcacgatatc agtaacggtc atcccatctg gctggcgtta
     7021 aaaaatgccg gtgagtttag cccgctctgt ttgcaattag tgagaacggg agaggcctcc
     7081 ggctcgctgg atctcatgtt agacaacatc gcccatcatc atcgggataa cacaatggcg
     7141 ctggcggata acctagcagc cttactggaa ccggcgttgc tgatcataac gggaggaatt
     7201 atcggtacgc tggtggtggc gatgtatctg ccaattttcc atttaggcga tgcgatgagt
     7261 gggatgggat aacgctggcg tgttgctcca caccagcgtt ggaagattac aggttgttga
     7321 agatgcggtt ttcttgttcc tgcacacgga taaatgtggt gcgcttggtc agttctttca
     7381 ggcgtgaagc cccaacgtat gtacaagctg aacgcaggcc gcccaaaata tcacgcgcgg
     7441 tattttcaac cgggcctcgc agcggcagct taacggtttt accttctgct gcgcgatatt
     7501 cagcaacgcc gccaacgtga cgtttcatcg cagactcgga gctcatgccg tagaacagca
     7561 taaatttctc gccgttctcc tcaacgatgc gaccgccgct ctcttcgtgg cccgccagca
     7621 tgccgccaag catgacgaaa tcggcaccgc caccaaaggc tttcgccaca tcgcccggcg
     7681 tggtgcagcc accatcgctg atgatcattc cgcccagacc gtgcgcagca tcggcacatt
     7741 cgattaccgc agaaagttgc ggataaccga cgcctgtttt gacgcgagtt gtacaaacag
     7801 aacctgggcc aatgccaact ttaacgatat cggcacctga gaggataagc tcctcacaca
     7861 tttcaccagt cactacgtta ccagcacaaa tggttttggt cggccacgct tcacgcgctt
     7921 tcgcaacgaa ctgcacgaag tgttcggaat aaccattcgc cacgtcaata caaacgaagt
     7981 ttaatgccgg gttcaggtcg agaatctgtt tagttttttc gaaatccgca tcagacgtac
     8041 cggtagaaac catcacatgt ttcagcacat cagcggaaga attgttgata aacgcttgcc
     8101 actcttcgac agaatagtgt ttatgcacag cagtcaaaat atcaaaagaa gccagcgcag
     8161 aggccataga aaatgtgcct acggtgtcca tatttgcggc gataatcggc acgccggacc
     8221 agctctgacc tgaatgtttg aaggtgaatt gacgttccag ttcaacatcg gaacggcttt
     8281 taagagtgga gcgtttaggg cggatgagaa cgtctttaaa acctaacttc agatcttctt
     8341 caatacgcat gtgcggattc ctggggttaa tggctataag ggtaagagcg caactccagt
     8401 gacgctatca tacgcactaa tcaatgcggc gcaagactgc gaaattgtct ttttttacgc
     8461 tacaatccca taaatttacc tggtaaatag tgagcgaacg tttcatcaat ttcgctttcg
     8521 tgcgctcatt tttaacgttt tgataatcat gatgaattcc cgggaatata aattatgagg
     8581 tatatagttg ccttaacggg aggcattggc agtggcaaga gtaccgttgc caatgcgttt
     8641 gctgatctcg gaattaacgt cattgatgcc gatattattg cgcgtcaggt ggttgaacca
     8701 ggtgcacctg cgctacatgc cattgctgat cactttggcg ctaatatgat tgctgctgat
     8761 ggaacattgc agcgccgggc cttgcgcgag cggatcttcg ccaacccgga agagaaaaac
     8821 tggcttaacg ccctgctgca tccgctgatt cagcaagaga cgcaacacca gatccagcaa
     8881 gctacttccc cctatgtact gtgggttgtg ccattgctgg tagaaaactc actgtataaa
     8941 aaagcgaatc gagtgctggt ggtggatgtc agcccagaaa cgcaacttaa gcgcaccatg
     9001 cagcgcgatg atgtaactcg cgagcatgtc gaacaaatcc ttgctgctca ggcaacgcgc
     9061 gaagcccgcc ttgccgtggc agatgacgtc attgataata acggcgcacc ggatgctatc
     9121 gcatcggatg ttgcccgcct gcacgcacac tatttgcagc ttgcgtcgca gtttgtctca
     9181 caggaaaaac cgtaatgcag acccaggtcc tttttgaaca tccactaaat gaaaaaatgc
     9241 gtacatggct gcgcattgag tttttgattc agcaactcac cgttaattta cccatcgttg
     9301 accacgctgg cgcgctgcat ttcttccgta atgttagtga attactggat gttttcgagc
     9361 gcggcgaagt ccgcactgag ctgttgaaag aacttgaccg gcagcaacgt aaactccaga
     9421 cctggattgg cgtgcctggc gtggaccaga gccgtattga agcattaatt cagcagttaa
     9481 aagcggcggg gagcgtatta atttccgcgc cgcgtatcgg gcaatttctg cgtgaagatc
     9541 gtttgattgc tctggtgcgt cagcgactga gcatcccagg cggctgttgc agctttgatt
     9601 tacctacatt gcacatttgg ctgcatctac cccaggcgca gcgcgactgc caggtagaaa
     9661 cctggattgc cagcctgaac ccgctcaccc aggcacttac catggtgctg gatttaattc
     9721 gccagtcggc ccccttccgt aaacaaacca gcctgaatgg tttttatcag gataacggtg
     9781 gcgatgccga cttgctgcgc ctgaatctgt cgctcgattc acagctttat ccgcaaattt
     9841 ccggacataa gagccgtttt gccattcgtt ttatgccgct ggacactgaa aacggacagg
     9901 taccggaacg tctggatttc gaactggcct gttgctaagg agtaaaaatg tcagaaacta
     9961 ttacggtgaa ttgcccaacc tgcgggaaaa cggtggtgtg gggtgaaatc agcccgtttc
    10021 ggccattttg ctccaaacgt tgtcagctga tcgacctcgg agaatgggct gctgaagaaa
    10081 aacgaatccc cagcagtggc gatctttccg aaagcgatga ctggagcgaa gagccaaagc
    10141 agtgacattt gtctgatgcc gcacgtaggc ctgataagac gcggacagcg tcgcatcagg
    10201 catcttgtgc aaatgtcgga tgcggcgtga acgccttatc cgacatacac acgcttaagc
    10261 ttcgcaatta ccggttcatt ggctggcgga aaatcatcgg cattaagacc gactagcgac
    10321 atccacttac cgggttgccc ttctttaccc cacggctccc cttcccagct ttcgaccagc
    10381 cagaaccaca gagttatatg cctgtccggg aattcatatt ccagtttttc aaatagcgaa
    10441 aaatgttgtg gggtaatccc gacttcttcc tgaagttcac gcaccaccgc ctgttccggc
    10501 gtttcaccca tttcaatttt accgccggga aactccagtt tattcgccat gtgcgcatct
    10561 gctgcgcgac gcgttataaa gatttcattg ttctcgttgc gaataatacc taccgcaatt
    10621 tgcagctttt tcattgtctt aaacctataa aaaaggcgca gaatcctgcg ccttttactt
    10681 caacagttag cttttattgc aggcggccat ggcactgctt gtatttttta ccagaaccgc
    10741 acgggcaagg atcgttacgt cctactttgc gctcaccggt ttgcgccgcc agtgcagctg
    10801 cggctgcaga gtcgtcatcc tgatggctaa gctgctgcat ttgcgctaaa cgctcggctt
    10861 ccatacgacg ctgttgttcc agctcctcaa cctcttcagg catacgtacc tgaactttgc
    10921 tcagcgtact gataacttca tatttcaacg actccagcat cgctgcaaac atggagaacg
    10981 attcacgttt gtattcctgc ttcggatctt tctgtgcata gccacgcagg tggataccct
    11041 gacgcagata gtccatcgct gccaggtgct ctttccacag ggagtcaagc gtttgcagca
    11101 tgacgccttt ctcgaagtga cgcatcatct cagcaccaac cacttcttct ttacgctgat
    11161 acacttcgat ggactgcgcc agaatgcgct cacgcagcgt ctcttcatgc agttctggtt
    11221 ctttatccag ccactcagca attggtaaat cgaggtcgaa atcgttcttc agacgttcct
    11281 gcagccccgg aatatcccac atttcttcca gcgactgtgg cggaatgtag gcatcaatgg
    11341 tcgctttgaa cacatcttca cgaatgctgt taatggtttc gctcacatcg ctgacatcca
    11401 gcaattcgtt acgctgggag taaatggcgc gacgctgatc gttagccacg tcatcatatt
    11461 ccagcagttg cttacgaatg tcgaagttac gactttcaac tttacgctgg gcgttggcaa
    11521 tcgctttagt cacccacggg tgttcaatgg cttcgcctgg cttcataccc agtttacgca
    11581 tcatgccgga tactcggtcg gaagcaaaaa tacgcatcag cgcatcttcc atcgacaggt
    11641 agaaacggga agaaccagca tccccctgac gaccagaacg accgcgcaac tggttatcga
    11701 tacgacggga ttcgtgacgc tcggtaccga tgatatgcag gccacctgct gccagtaccg
    11761 catcgtgacg tacctgccag tcggctttaa ttttttcaat ttgctctgcg gtcggatttt
    11821 ccagcgcggc aacttctgcc tgccagctac caccgagcac aatatctgta ccacgacccg
    11881 ccatgttggt cgcgatagtc accgcagccg gataacctgc ctgagcaaca atcgccgctt
    11941 cgttggcgtg gaatttggcg ttcaggacat tgtgcttaat accggctttg gtcagttcgt
    12001 ttgacaccag ctccgatttt tcgatggaga tagtacccac cagcaccggc tggcctttcg
    12061 cagtacgttc tttgatatct tcaatgatcg cctgaatttt ttccgcttca gtcatgtaga
    12121 ccaggtccgg cagatcttta cgaatcattg gacggttggt cggaacaacg acggtatcca
    12181 gtttgtagat ggagctaaat tcgaaagctt cggtatcagc agtaccggtc atccccgcca
    12241 gtttttcata cagacggaag tagttctgga aggtgatcga agccagcgtt tggttttcgt
    12301 tctggatctg cacaccttct ttcgcttcca cagcctggtg cagaccatcg gaccagcgac
    12361 ggccctgcat ggtacgaccg gtgtgttcgt caacgatgat aacttcacca tctttaacga
    12421 tgtagtcgac gtcacgggta aacagcgcat gagcgcgcag cgccgccgtt acgtggtgca
    12481 tcagcatgat gttggccgga gagtacagag actccccttc atccatgatg ccctctttca
    12541 ccagcagttc ttcaatcagc accagaccac gttcggtcag gttcacctgg cgagattttt
    12601 cgtccaccga gaagtggcct tcgccctgga aggtttcgga gtcttctttt tcctgacgga
    12661 tcaggtgcgg aataatttta ttcacgcgtt tatacatttc cgagctgtct tctgccgggc
    12721 cggaaatgat cagcggtgta cgcgcttcat cgatcaggat ggagtccact tcgtccacca
    12781 gcgcatagtg cagtttacgc tgtacacgtt cttcagggct gaacgccata ttgtcgcgca
    12841 ggtagtcaaa gccgtattcg ttgttcgtac cgtaagtgat gtcagctgcg taagcttcgc
    12901 gctttgccgg tgctggcatg cccggcaggt tgataccgac agtcaggcca aggaattcaa
    12961 acagcggacg gttgttttcg gcgtcacgtt gcgccaggta gtcgttgacg gtaactacat
    13021 gcacgccttt accggttagt gcgttcagat aagcaggcag cgttgcggtc agggttttac
    13081 cttcaccggt acgcatttcg gcgatgcagc gttcgttaag aaccataccg ccgagtaact
    13141 gaacgtcgaa gtgacgcata ccaaagacgc gcttacttgc ctcacgtacc acggcgaaag
    13201 cttccgggat cagattttcc agcacttcgc ctttttccag acgtgcacga aactctgcgg
    13261 ttttcccttt cagttcttcg tcggagagtt tttccatctc cggttccatg gcattgatga
    13321 tgttgaccac tttgcgcatc cggcgcaggg tgcgatcgtt acgactaccg aaaactttag
    13381 ttaataattt gattagcata ataaaatctc aaacgccccg cgttgcggag ttaataaaat
    13441 gaagtaaagg tttattgttg ttagctgagg cgttgagggc cagcacggat gccttgcgcc
    13501 tggcttatcc agacgggcgt gctgaatttt gcttgtgggg taaaatgcgc ataatcaatg
    13561 cgataaccct tttcagacgg cgtgccttcc tgggtcagca gcgcgctgag cgtatccagt
    13621 aatgcaagat gttgcgcctg aagaggcaaa gattcttcag caacgggcag cgtttgcggt
    13681 gccattgcga aggaaagatg acggattacc gtgcgaatgg catgttgatg ccagtaatct
    13741 acggaatagt tcgaattcgg gcggcgcgtg ttcgcttcca gcaaggccaa ttgaccaaag
    13801 ttaactttgg ctgaaggctc gtggttgcgg gttgtcgctt ttgcgggcgc gtttggttcg
    13861 gcggcgttgc tgagcgcagg caaacctaaa ctcgccgcaa ccatccctaa taagagatgc
    13921 ggccagaagt agcgtttacc aaactgtcgc cagcgcgtca gtattccact cacgttattg
    13981 ccatcccgat cgtattcaaa cgtatttagc gcacaaatct tatcattaaa gcctgaagtc
    14041 cacagcacga atgaagataa gttgtccgta aaagtgctta agaaaacagc gttcgcaccg
    14101 ttttttatag ctcttataaa attcaaaaaa aaacgactgg ttcacctggc cggagagagt
    14161 gccagatttg ccagtcgaat tttatacgac agtataaatg tcgttatgcc agtacggctg
    14221 aaggcgcttt gaaggccaac ggcagttctg cgtcgtcctg gaaggtcaca tattcccagg
    14281 cttcctgttt cgccaggaca gcctgcagca gtttgttatt cagtgcatga ccggatttat
    14341 aagcggtaaa tgcaccaata atattgtgac cacacatgaa caagtcaccg atcgcatcga
    14401 gcattttgtg acgcacaaat tcgtcttcaa aacgcaggcc gtcttcgttc agtacgcgat
    14461 aatcgtcaac aacgatggca caatcgaagc tgccgcccag gcacaaacca cgggactgca
    14521 gatattcgat atcacgcatg aaaccgaacg tacgcgcacg gctgatctgg cgcataaacg
    14581 catcagcgga gaagttcatc gcatagcgct ggttgctgga atcaatagcc ggatggttaa
    14641 aatcgatggt gaaatccagc gaaaaaccat tgtacggctt aaattcagcc cacttatcgc
    14701 catcttcgac acgaacagtc tctttgatgc gaacaaattt tttggcgcag ttcaactcgt
    14761 cgataccggc gtcaagcagc aggtatacaa acggagcggc gctgccgtcc atgatcggga
    14821 tttccggcgc gttaacttcg ataacaatgt tatcgatgcc caagcccgcg agagcagcat
    14881 tgaggtgctc tacggttgaa atccgtacat catgctcgtt gaccagacac gtacagagca
    14941 tggtatcacg cacagatttg gcatcggccg ggaaatctac cggtggattc aagtcggtgc
    15001 gacgatagat gaccccggtg ttggccggcg cagggcgtaa cgtcagggtg actttcttgc
    15061 cggtatgtaa accgacaccc gtcgcctgaa cgatacgttt aagtgtcctt tgtttgatca
    15121 tcgtattatc tcgccaaatt acctatccaa ccgaagtgta ctatacattc ggcgggccag
    15181 tttagcacaa agagcctcga aacccaaatt ccagtcaatt cttaatcagc ttgcttacgc
    15241 aggaatgctg ggatatccag ataatccggc tctttcgcag tttgcggcgc attgtcattc
    15301 acgactttag caaccggctt ctgctcctgg gtcagcggag ccatcccatg ctgctggtag
    15361 cgatccatca ctggctgctg aacctgctta ttggtcacca gagtgatttc aggacgtttg
    15421 tccatgccga tacctgtcgc aacaacggtt acgcgcagct cgtcattcat atccgggtca
    15481 agagaagtac cgataaccac agtcgcgttg tcggaagcaa atgcacggat ggtgttacct
    15541 accgtttcga actcatccag acgcaggtcg aagcccgctg tgatgttaac cagcacgccg
    15601 cgtgcgccag acaggtcgat atcttccagc agcggagaag agatagccat ttcagcagct
    15661 tcttccgcac ggtcttcacc gctcgccacg ccagaaccca tcattgcgta gcccatttca
    15721 gacattacgg tgcgtacgtc tgcaaagtcc acgttcatca aacccggacg agtaatcagt
    15781 tcggcgatgc cttgcacagc gcctttgagt acatcgttcg ctgcgccaaa cgcatccagc
    15841 agggagatac cgcggcccag aactttcagc agtttgtcgt tcgggatagt gatcagagag
    15901 tccacatgct tggacagttc agtgatcccc tgctccgcga atgccatacg cttcttgcct
    15961 tcaaagttga agggcttagt gacgacagca acggtcagga tacccaaatc ttttgccact
    16021 tcagcgacga ctggtgctgc acctgtaccg gtaccaccac ccatacccgc agcaataaag
    16081 accatgtctg caccttccag cgccgcacgc agtgcatcgc gatcctcatc tgccgcattg
    16141 cggccaactt ctggattagc gccagcgccc agtcctttgg tgataccgct accgatttga
    16201 atcgtctgtc caaccgctgt tttacgcagc gcttgtgcat cggtatttac cgcgaagaat
    16261 tcaacacctt caatgcgctc gcgcaccatg tgttcaacag cattaccgcc gccgccgccg
    16321 acgccgatga ctttaatcac cgcgtcattg gtaagttcca ttggttcaaa catagtttct
    16381 ctccgatttg tgcctgtcgc ctgaggccgt aatcatcgtc ggcctcataa aaattaaaac
    16441 tcttttcgca gccaactatt gagtcgcttg atccacgagc caactgatgc tgtaacacgt
    16501 ttttctactt cagcttcacc gttaagatgt gactctttcc catagtgaag caatcccacc
    16561 gccgtcgaat aatacggctc ctgagcataa tccgttaaac cggtaatgtt cagcggcgcg
    16621 ccgatacgca cttgcgtatg aaacacgcgc tgagcacagg ctgcaagacc ttcgatctgc
    16681 gctgcgccac cggttaatac aatgcctgcc gccaggtgat gtttaacccc ttgttggcga
    16741 agcttttcct gcaactgcaa tatctcttcg ttgaccaggt tgagcagctc ggtatagcgc
    16801 ggctcgatca cctctgccag ggtctgacgt tgcagactcc gcggcggacg accgcctacg
    16861 ctcggcactt ccacgctctc atcttttcca acgatggaac ccagcgcaca accgtggcga
    16921 actttaatcg cttcggcgtc gcttggcggc gtgccaaagg cgtaagcgat atcactggtc
    16981 acgacattgc cagcataagg aattacctta gtgtggcgca atgccccacc ggtataaacg
    17041 gcgatatcca ttgtaccacc accgatatcg acgacgcaga cacccagttc acgttcatct
    17101 tccgtcaata ccgaataact tgatgccaga ccggcaaata tcagttggtc aactttcagc
    17161 ccacaacgtt caaccgcttt gacgatgttt ttcgccatat cgttgtggca tgtgatcagg
    17221 tgcacttttg cctgcatccg cacgcccgaa agccctaccg gattcttgat cccttcctga
    17281 tagtcaatcg catactcttg cgggatcaca tgcagcacac gatgctcatc gcgtacacgc
    17341 accgatttcg cggtatggac gacgttttcc acatcttctt gcgttacttc ttcttcagaa
    17401 ataggcacca taccaatttc attctggcag ctgatgtgct taccagaaag cgccagatat
    17461 accgaagaga tctgacaatc tgccatcaat tctgcctggt caatggcacg ttgtacgcac
    17521 ttgaccacgg attcgaggtc gttcaccccg cctttatcca taccacgcga cgggcagctg
    17581 cccacgccaa tgatattgac cataccgtcg ggcagaactt cccctactaa agcggcaacc
    17641 ttcgcggtac caatctccag tcctactacc agttttctgt ccgtcgcctt gatcattgtt
    17701 gttctgcctg tgcctgattt tgttgctgag tagattcctc tggcggcaag ggcgcccagc
    17761 ctactgccgc tccagagtca taacgcaaat caacgtagct aatccgtttg ccatcggttt
    17821 gcgcctgctg ctgtagaacc ggataaagtt ctacaaagcg agccaaacgt ttcatcgtat
    17881 cgccccggcc aagattgagc ttaatatcgt tattcagcgt caactgccag gaacgccgcg
    17941 cggtcatcgc cgcttccttc agagtaaatc tgtcctttgc cagcatctgc cccatttcgc
    18001 gatagccctg caacacttca ttggcgctgc cttccgggcc atacagcatt ggaagcacct
    18061 gcttgctggt gcgatctggc ggcacgctga aggtatttcc ttccgcgtct accatgtgtt
    18121 gatcattcca ccgcgcaatc ggcacatatt caaccagatg aatcttcaat tcatcaggcc
    18181 actgctttct gacgctcacc tgcttaatcc acggcagacg ttgttctatt tgcgtctgga
    18241 tgatgttgac atcctgggtc ataaaggtac ccggctcacc caatgccagg atcgactgcc
    18301 ggatatcgtc attacgcgtg taatggcgtt caccggtcaa caccagcttt gagagcggca
    18361 ggcgttgcgc atcttccatc cagcccaaca cgacccagcc gctcaccaac actgtcgtta
    18421 aaacggtcag caggaaaagg atccccgcca gacgcgttcc attattgcgg cgagaagaaa
    18481 cctcttcttc gctgtttcgc gtgttcagag cagcctgcga catattagtc cgccagttcc
    18541 agaattcgta ctaccaactg cgagaagctc atacctgcct gacgtgccgc catcggcacc
    18601 aggctgtggc tggtcatacc cggtgaggta ttggcttcca gcagataaaa ctgtccatcg
    18661 ctgtccagca taacgtcaat acgtccccat cctttgcaac ctaacgtcgt ccatgctttc
    18721 agcactaatg cctgcaaatt ggcctcttgt gacgcttcca gacctgcggg gcagaaatac
    18781 tgtgtctcat cagagagata cttcgcctca taatcataga aggttccgga cggttgaata
    18841 cgtactgacg gtaaaatttc ttcaccgagt atcgcaaccg tgaactccgg cccacttagc
    18901 catttttcaa tcaatacttc ttcatcgtgc tgaaatgcca atcttaatgc atcttgtaga
    18961 gcattttctg ctactacttt tgacattccc acactggaac cttcgcggct cggcttaacg
    19021 ataaccggca aacccagagc agaaatttct gctaactgct tatcactcag gcctttttca
    19081 aactctacgc gggttaacgc tacccacggc gcgaccggta aaccggcacc ttgccacaga
    19141 agtttgctgc gtagtttatc cattgaaagc gcagatgcca tcactccgct gccggtataa
    19201 ggcaagccca tcagctcgag catcccctgc agcgtaccat cttcaccgcc gcggccgtgt
    19261 agcgcgataa acactttctg aaagcccatc gacttcagtt gcgtcacgtc aacttctttc
    19321 gggtcgacag gatatgcgtc aataccgcct tcacgcagcc cggctaacac cgctgcgcca
    19381 gaattcagag aaacttcccg ctcagcggaa gtcccgccca acaggaccgc gattttatca
    19441 gtcatgttgt tcttcctccg gagtttgcgg cttcagtttg atttcagcta aagaacgggc
    19501 aatttttccg atattaccag ccccctgaac gagaatcagg tcgttaccgg ttaataccgg
    19561 tgccagcatc tcggctacct gcgcagggtc cggcaccaga atgggatcta ttttcccacg
    19621 tccacgaatt gtgcgacaca gcgaacggct gtccgcaccc ggaattggcg cttcgccagc
    19681 cggatacact tccagcatca acagggtatc aacctgcgtc agcacattgg cgaaatcatc
    19741 atacaggtcg cgtgtacggg taaaacggtg cggctgaaac agcattacca ggtttttatc
    19801 cggccagcct gcgcgcgccg ctttaatggt ggcgtccact tccgtcgggt ggtggccgta
    19861 gtcatcgacc agcattgccg taccgctttt accattcact ggctccagcg ggaattcacc
    19921 gaggaaatca aaacggcgac cagtcccctg gaagctctcc agcgcacgca aaatagcctc
    19981 gtcgtcaatg ccctcttccg tagcaaccgc aaccgcagct gcggcgttca gcgcgttatg
    20041 acggcctggc gcattcaggg tgacgcgcat cggctctttg tcctggcgca gcagcgtaaa
    20101 gtgcccctgc gggccaatct gctgataatc ttctacacgc acgtcggcat cttcgctgaa
    20161 gccgtaagtt gtggtctgac gtcccactcg cggtaacaat tcgcggatca ccggatcatc
    20221 aacacacatc accgcacgac cgtaaaacgg caggttgtgc agaaaattaa taaaagtctg
    20281 ttttaaattc tcaaagtcgc cctggtaggt atccatgtgg tcggcttcga tattggtgac
    20341 aatcgccacc atcggttgca gatgcaggaa cgatgcatca ctctcatctg cttcggcaat
    20401 caggtaccga ccatgcccca aacgcgcatg aacccccgcc gcttttacca gcccgccgtt
    20461 aacgaaggtt gggtcgagcc ccgcttctgc gtagatgctg gagaccatcg cggtagttgt
    20521 cgttttgccg tgcgttcctg caatggcgat gccatgacga aaacgcatta actcagccag
    20581 catttcggca cgacggatca ccggaatacg cgcttcatga gctgcgacaa tttccgggtt
    20641 atcggcagaa atggcgctgg aaacaacgac cacgctggca tcacgtacgt tttccgggcg
    20701 atggttgaaa taaatcgtcg ctcccagatt cattaactgc tgcgtgaccg gatttggcgc
    20761 taaatcggaa ccactgatct gataaccttc attggccaga acttcggcaa taccgcccat
    20821 accggcacca ccgatgccga caaaatgtat gtgccgaacg cgacgcattt cgggcacgat
    20881 ggaacgcagt tttgccaatt gttgtgtatt cattctttac gccattaact tcttaaattc
    20941 atacgatgca aaaggcatcg ctacaattac gcccgggcag cccgactcac ttcatttgcc
    21001 actcgctcgg tggcatccgg aatggatgca gcgcgggcgc gttctgccat ggttaataag
    21061 gtttctcgcg accacccggc cagggtgttg gcgacagcat ccacgctaag ctgtggctgc
    21121 tcgataattt tggctgcgcc cgctttttcc agcggtagcg cattccagta ttgctggcgg
    21181 tctttatgtt gaaacggcac aaacaacgcc ggtagtcctg ccgcggcgat ttcactcacc
    21241 gttaaagcac cggagcggca aacgacgaca tccgcccacg catacgccgc cgccatatca
    21301 tcaataaatt cagtcacttt atgctgcggt tgccccgctt cggcatacgc ctgttcaacg
    21361 gattgttgcg aacctttgcc gctctgatgc cagatagtga ctgaatcacc cagtttcgca
    21421 gcaacctgcg gcattgtctg gttaagaatg cgtgcgccct gggaaccacc cactaccagc
    21481 acacgaaccg gaccttcacg tccagccaaa cgttgctgcg gcaacggtag cgccaacaca
    21541 tcggtacgca ccgggttacc caccacttcc gcattgggga aagcacctgg aaacgcctgc
    21601 atcactttgg tggcaatctt cgccagccat ttattggtta agcccgcaat accgttttgt
    21661 tcatgaagta caaccggaat gcctaacgac cacgcggcca gaccaccagg acctgatacg
    21721 tagccgccca taccgagcac cacgtcaggt ttgtacgctt tcataatcgc ccgcgcctga
    21781 cgccaggcat tgaagatacg cagcggcgca gctatcagtg cttttatacc ttttccacgc
    21841 agaccagaga tacgaatgaa atcaatttcg atgccatgtt ttggcactaa gtccgcttcc
    21901 atacggtcgg cagtccccag ccagcgaact tgccaaccct gagccattag atggtgcgca
    21961 accgccagtc ccgggaatac atgtccaccg gttccgcctg ccatcaccat taatcgcttt
    22021 ccttgagcac tcatcgtgaa cctcgtacaa acgcctgcgc tttctccaga cgcgtttcat
    22081 aatcaatacg caacagcatc atgatggctg tcgacataat cagtaagctc gaaccaccgt
    22141 aactgatcag cggcaatgtc agacctttgg tcggtaacat ccccgccgcc gcgcctacgt
    22201 taaccagcgc ctggaagcta aaccagatgc caatagaaca ggcgagaaaa ccggaaaaac
    22261 ggtggtcaat ttctaatgct ttacggccaa tcgacatcgc gcgaaaagcg acgaagaata
    22321 ccattaaaag tgccagcacc acaccgacat accccagttc ttcgccgata atggcgaaaa
    22381 taaagtcagt gtgcgcttcc ggcagatact ccagtttttg tacagagtta cctaaacctt
    22441 gtccccacag ttcgccgcga ccaaacgcca tcagcgattg cgttaactga tagccgctgc
    22501 caaagggatc ttcccacggg ttccagaatg cggtaacacg gcggatacgg tacggttcgg
    22561 cgagtatcag caacacaacc gctgaaatgc ccataccgat aatggcaatg aactgccaca
    22621 atttcgctcc cgccaggaac aacatcgcca gcgtagtcac aaacaacacc accaccgtac
    22681 caaggtctgg ctgtgccagc agtaacactg ccaacaccag aatcacgccc atcggtttca
    22741 ggaagccgcg caggttatta cgtacttcgt cacctttacg caccagatag ttggcgatat
    22801 agcaaaacag cgacagtttt gtcagctcag caggctggat acgcagcaaa ccgagatcga
    22861 tccaacgcga tgccccttta accgagctcc ccactaccag cacgatcatc agcaggatga
    22921 tagatccgag cagcatcgtg gcactgtagc gttgccagaa ctccatcggc agacgcagcg
    22981 taatgatcgc cagaataaac gccaaaatca gatagacacc atcacgcttc gcgaagaaga
    23041 acggatcgtt ggttaagcgt tgccctatgg gcattgacgc cgaggtcacc atgataaagc
    23101 caatcgccgc gaggccgaag gtcagccaca gtaaggtgcg atcgtacatg atcaggctgt
    23161 cggtatcttt ttcccgcgag cccatcaccc agccctttag cgccgtggag atccagacca
    23221 ggatactgaa tcctggcagg cgcggcattt tcaggcgagg gagagataaa cgcatcaacc
    23281 taactccttc gccagacggg caaactcatt gcctcgttgt tcaaagttct tgaactgatc
    23341 aaggctggca caggccgggg agagtagaac catatcgccc ggctgaacac gcggagccag
    23401 caagcgcatc gcctgttcca tagtttcggt ttgttctgcc acttccgggc gtagcgccgc
    23461 cagctgcacg ccgtcacgac cgaaacaata cagacgtacg ttatcgccat tcaggtaacg
    23521 cgccagtggg ctaaagtccg ccgatttacc atcgccgccc agcaacaaat gcagcgtgcc
    23581 gtctacgtgc aggccattca gcgccgcttc cgtactgccg acgttggtcg ctttcgaatc
    23641 gttaatccaa cgtacgccgt tatgctccag cacaacttca aagcgatgcg gcagaccagt
    23701 gaatgtggtt aacgctttca ggctgctggc acgcggtaac cctgcggcat ctgccagcgc
    23761 cagcgccgcc agcgcattgg tgtagttatg ctgcccggaa agtttcattt ctttcacgtt
    23821 cagcactttc tcgcctttca cccgcagcca ggtttcgccc tgctgatgat tcaggtgata
    23881 gtcacccatg ttgacgccaa agctgacgca gcgttcatcc gcaccgcgaa tcggcattgt
    23941 taaggcatca tcagcattga cgacacaaac tttcgcattt tcgtaaatac gcaattttgc
    24001 cgcacggtat tgctgtaaac caaacggata gcgatccata tgatcttcag tcacgttcag
    24061 aatggtcgct gctaccgcct gtaagctgga ggtggtttcc agctggaagc tcgacagttc
    24121 cagtacgtac agttcgcact catcatccag cagcatcaac gcgggcaggc caatattgcc
    24181 acccacacca acgttaaccc ccgccgcttt cgccatttca cccactagcg tggtgaccgt
    24241 gcttttgccg ttagaaccgg taatcgccac aatcggtgct tgtgcttcgc gacagaacag
    24301 ctcgatatcg ccaacgattt cgattccggc atcagcggca gcgcttaagg atggatgcgc
    24361 cagtgcaata ccgggactgg cgacaatcag atctgccgcc atcagccatt catcattcag
    24421 actgcccgtg tggcgttcta cggcttcggg taatttatcc aggccaggcg gtgtcatacg
    24481 cgtatccata acgcgcggcg tcacaccgcg agcgaggaaa aagtccacgc aggaaagccc
    24541 ggtgaggccc aggccgataa tgacaacatt tttaccctgg taatcagcca tgattaacgt
    24601 accttcagcg ttgccagacc aatcaggacc agcatcagcg aaataatcca gaaacgcaca
    24661 atgacgcgcg gttccggcca gcctttcagt tcatagtggt gatgaatcgg tgccatgcgg
    24721 aaaatacgtt gtccgcgcag tttaaaggag ccgacctgca ggatgacgga aagcgtttct
    24781 accacgaaca cgccccccat aatcaccagc aggaattcct gacgcagcag tacggcgata
    24841 atgcctaacg caccacctaa cgccagcgaa cctacatcgc ccataaagac ctgcgccgga
    24901 taggtgttaa accacaggaa gcccagccct gccccgacta tcgcggtaca gacaataacc
    24961 agttccccgg cgtgtcgcag atatggtatg tgtaaatagc tggcaaagtt catgttgccg
    25021 gtcgcccacg ccaccagcgc aaaaccaccg gcgacaaata cggtcggcat aattgccaga
    25081 ccgtcaagac catcggtcag gtttaccgcg ttgccagtac ccacaatgac gaagtaagcc
    25141 agcagaatgt agaacagccc cagttgcggc atcacatctt taaagaatgg gaccaccagc
    25201 tgcgttgcgg gcgtgtcttt gccaacaagg tacagcgcga aggcgacacc cagcgcaatg
    25261 accgacatcc agaaatactt ccaacgagcg atcaaccctt tggtgtcttt acgcactact
    25321 ttgcgataat catcaacaaa gccaataaca ccgtaaccta ccagcaccac caacacgcac
    25381 cagacgtacg gattggacgg gtaagcccac agcagtacgg agatcacaat cgccgtcagg
    25441 atcataatcc cgcccatggt cggcgtaccg cgcttgctga agtgtgattc aggaccgtcg
    25501 ttacgcacca cctgaccaaa ggaaagtttt tgcaaatgag caatcatacg cgggcccatc
    25561 cacaatgaga tgaacagcgc ggtcagcagg ctgacgatgg cgcgaaacgt cagataggaa
    25621 aagacgttaa agccggaata atatttgacc aaatgttcgg ccagccaaac taacatgtcc
    25681 cattctcctg taaagcgcgt actacctctt ccatggcggc actacgtgaa cccttaacta
    25741 aaatcgtaat tacctgttgc tcagcaatca gtgatttaag acgcgtaatt aacgcagttt
    25801 tctcagcaaa atgttcgcca acgccgctgg cggtgctgat agcatggctt tgtttaccca
    25861 cgcttaacac gcggtcaata ccagcagctt ttgccgcctc gcccacctgt acatggcagg
    25921 cttcgctttc agcgcccagt tccgccatat cgcccaccac cagcacgcgg tagcccggca
    25981 tttcagccag tacctgaaca gcggcggtca ttgaaccgac attggcgttg taggagtcgt
    26041 cgagcagcaa ctggttttct gccagttgaa tggggaacaa acggcctgga acagctttca
    26101 gatttgccag ccccgctttg atagcatcaa gcgttgcgcc cacggacatg gagagcgcag
    26161 cggctgccag cgcattcgca atattgtgac gccccggcaa cggcagcaga acatccacgc
    26221 taccggttgg ggtttgcagg gtaaattccg taccgtgcga agtcacatgg atattggtgg
    26281 cggtgaaatc gctgttggcg gcattaggtg agaaacgcca cactttgcgt gagccaatta
    26341 cgctctgcca gttcagccag tcgttgttgt cggcgttcat aatggcgata ccgttttccg
    26401 gcaggccgct aaagatttca cctttcgctt tcgcgacacc cgcaagcgag ccaaaacctt
    26461 ccagatgcgc cgctgccagg ttgttgacca gcgcagcttc cgggcgagtc agactcacag
    26521 tccagacaat ttcgccctga tggttcgcgc caagttcaat aactgcgtaa tcgtattccg
    26581 gcgttaagcg caacagcgtc atcggtacac cgatgtcgtt gttgagattg cctgccgtat
    26641 aaagcgtgtt gccgcactgg cttaaaatcg ccgccgtcat ctctttaacg gaggttttgc
    26701 cggaggaacc tgtcagagca accacgcgcg ccggaacttg ctggcgaacc catgcagcca
    26761 gttcaccaaa cgccagacgc gtatccttga cgattaactg cggcaggtcg atatccagcg
    26821 gacggctaac cagtagtgcg cctgcgccgc cagctttcgc ctggtcggca aaatcatgag
    26881 catcgaaacg ttcgcctttc agggcaacaa acaggcagcc cggcgtcagt tttcgggtat
    26941 cagtggttac agcatcaagg gtgatatctg caccttgcag ttcaccgttg agaatgtcgg
    27001 taagttggct aagggttacg ctaatcatgc aatcaccccc agcagacgcg ccaccgtgac
    27061 gcgatcggag tagtccagac gctgattgcc aacaatctgg taatcttcat ggcctttgcc
    27121 cgcgaccagc accacatcgt tctctttagc ctgcataacg gcgcaagtca ccgcttcagc
    27181 acggtcttcc atcactttgg catgtccggc atctaacatt cccgccagaa tatcgttgat
    27241 gatggcacgc ggctcttcgg tacgcggatt atcgtccgtc accaccgcca catcagcaaa
    27301 ctcttcggca attgcgccca tcagtggacg cttaccttta tcgcgatcgc caccacagcc
    27361 aaagacacac cacagcttgc ccgcacagtg cagacgcgcc gcctgtaagg ctttttccag
    27421 tgcatccggc gtatgcgcgt aatccaccac caccgtcggt ttgcctggcg cagtgaacac
    27481 ttccatacgt ccgcaaaccg gttgcagacg agcagcggtt ttcagcagat cagccagtgg
    27541 atagcccagc gccagcagcg tcgccagcgc gagcagcagg ttgctgacgt taaaagcgcc
    27601 catcagatgg ctttcaattt cgccatcgcc ccaacttgag ctaaagcgaa tagtcgcacc
    27661 gctgtcgtga tagttcactt cggtcgcttt caaccagcgt ccgtgacagt tcggattgat
    27721 atgatcttcc attgataccg caactgcatc cggcagtttt gccagccagc ggcggcctac
    27781 ttcatcgtcg gcattaataa tcgcctgacc gcaatgatgt tcagaataaa gcagccattt
    27841 cgcagcttcg tagtgttcca tatcaccatg ataatcaagg tgatcgcggc ttaagttagt
    27901 aaagaccgac gccgcaaatt tcaatgccgc cacacggtgc tgtaccagcc cgtgggagga
    27961 aacttccatt gcgcaaaaag tcgcgtcctg atccaccagc cccgccagct catgctgaac
    28021 atcaactgcc gaaccggtgg tattttctgt cgggatcact ttccccagca gaccgttacc
    28081 aacggtgccc attaccgcgc tggtttcgcc aagcagttgg ctccactgcg ccagcagctg
    28141 ggtcgtcgtg gttttgccgt tggtgcccgt tacgcccacg agacgtaaat tgtcagaggg
    28201 ttcatggtaa aagcggcccg ccagtgcaga taaacgctcg ttgagctggc tgagatagat
    28261 gaccggtacg ccgtgcattt cacggatttc accgtcggtc gcctcacctt tcgcctctgc
    28321 aataatggca gccacacctt gcgctatcgc ctgcgggata tatcgacgcc cgtccgcctg
    28381 atgacctact acagctacaa agagatcgcc cgccgcagcc acacggctgt cgagtgtcat
    28441 ctctcgcagt gctcgcgaag gtgcgtctgg cacccacgga gcaagaaggt cgcgcaaatt
    28501 acgatctgcc acctgtcccc tcgccttgat taatcacaaa ttcattttta tcgcccgttg
    28561 tcagcgcatc cggctcgatg ttcatggttc gcaatacgcc gcccatgatg gcaccaaaga
    28621 ccggcgcgga aacggcgccg ccgtagtatt tacccgcctg cggatcgttg ataacaacaa
    28681 ccagcgcgaa gcgcggctga ctcgcaggcg caacacctgc ggtgtaagca atatatttat
    28741 tgatgtagcg accgtccggc ccgacctttt tcgcggtacc ggttttaatg gcaatacgat
    28801 agcctttaat cgccgccttc acgccgccgc cacccggtag cgccacgctt tccatcatat
    28861 gcaccacagt gcggacaata gattccggga agacacgttc accgggaacc ggggggtcaa
    28921 ctttggtaat cgacagtggg cgataaatgc cgtagctgcc gatagttgcg tagactcgcg
    28981 ctaactgtaa cggagttacc attagcccgt agccgaaaga gaaggtggcc ctctctatgt
    29041 cagaccaccg ttgtttttga ggatataagc cactgcgttc tccgaccaac cccaaattgg
    29101 tcgcttttcc cagtccaaaa cgtgagtaag tatctactaa cgctgaggac ggcatcgcta
    29161 acgccagctt ggaaacaccg acgttactcg acttctgtaa tactccggtc agggttaatt
    29221 cgctgtagcg tgccacgtct ttgatttcgt ggccgttaat tcgataagga atggtattga
    29281 gtaccgagtt ttcccgcacc acgccacgtt gcaacgcggt catcaccacc atcggtttaa
    29341 ccgttgaacc cggttcaaac acgtcggtga tggtacggtt acgcatcgcc tctttcggcg
    29401 tgccgctcag attgttaggg ttgtatgacg ggctgttagc catcgccagc acttcaccgg
    29461 tgttgacatc caccagcacg gcgctaccgg actcagcctt gttaaaggcc accgcgttgt
    29521 tcagttcgcg ataaaccagc gcctgcaggc gttcatcaat actcagcgcc aggttgtgcg
    29581 cagcctggct gtcagtagaa gaaatatctt caattacgcg accatagcgg tctttacgca
    29641 caatgcgctc acccggctgc ccggtaagcc atttatcgaa actcttctca acgccctcaa
    29701 taccttgact atcgacgtta gtaaagccga tgaggtgagc agtcacttcg ccggatggat
    29761 aataacggcg agattcttca cgcagatgaa ttcccggcag tttcagtttt ttgatgtagt
    29821 ccgccatgtc agggtttacc tgacgcgcca gataaataaa gcgccctttc gggttggcgt
    29881 taatgcgggc agaaagctga tccagcggaa tattgagcgc gttagccagc gccttccagc
    29941 ggtcaccgac gctgataccg ccagcgtcat gcacttcttt cgggtcagcc caaatcgctt
    30001 ttaccggcac gctcaccgct aacgggcgac cagaacggtc agtaatcatg ccgcgggagg
    30061 tggaaacttg ctgaacgcga agagaacgca tgtcgccctc tttcaccagc atatccgggg
    30121 agataacttg taaccacgct acgcgtccga gcagaaaagc cagcgccagg agaatacagc
    30181 cgcataacaa cgcaaaacgc caactgataa agttggcatg ttcttcctga cgttttggtt
    30241 tctgcgtttt cgccgctgct ttcatgcgtc gcgtttatcc ttatttttgc actacgatat
    30301 tttcttgtga cggatcaaca tgctgcattt gcagcttttc cgtggcgatc ctttccaccc
    30361 ggctatggtc gccgagcgca ttctcttcaa ggatcaggtt gcgccattca atgtctaaag
    30421 catctcgctc cagcaccagt tgttcgcgct gagcggtcag taaacgggta tggtgcgccg
    30481 tggttaccac ggtcaccgcc gtcaaaataa tgcaaatgaa caggcagagt ggcagcttcc
    30541 caaatcgcaa aagatcgtca ccgataacac caggcaatgc atggcgctcg tggcttccca
    30601 tcgatccttt aactttgctt agagcttctg tcactctgct gatcatgcgt tcgtcctctc
    30661 tgcaatacgc agaactgaac tacgggcacg aggattctca gccacctctt cttcgcccgg
    30721 cattaatttg cctagtgctc gcagctgacg gccacccagt tttttgagct gctcttcagt
    30781 catcggtaac cctgccggaa cttgcggacc gcggctgttt tcacgcataa aacgtttcac
    30841 aatacggtct tccagcgagt ggaagctgat gatcgaaagc cgcccacccg gggccagcac
    30901 gttgagcgag ctttttagcg cctgctctat ctcctccagt tcactgttta cccaaatgcg
    30961 caccgcctgg aaggtacggg tcgcgggatg tttaaactta tctttcaccg gcgttgcagc
    31021 agccacgact tccgccagtt ctttggtgcg ggtcatcggc tgttcgcggt tacgctcgac
    31081 aatggctcgg gcaatgcgtt tggcaaaacg ctcttcacca taggttttca atacccaggc
    31141 gatatcggct tcttctgcgg tttgtagcca ttcagcggct gactgcccac gggttgggtc
    31201 catacgcatg tccagcggac catcgcgcat aaaggaaaag ccgcgttcag catcatcaag
    31261 ttgcggtgaa gagacaccaa gatcgaggag aatgccgtcg atcttgccga taagatcgcg
    31321 ctcggcaacg tattcgccca gcgcggaaaa aggtccgtgg atgatggaga agcgcggatc
    31381 atcaatagtc ttcgcaacgg cgatagcctg cgggtcgcga tcgatcgcca gcaaacgccc
    31441 ctcttcgcca agctgcgaga ggatcagacg tgagtgacca ccgcgaccaa aagtcccatc
    31501 aatgtagatg ccatcaggac ggatattgag gccattaacg gcttcatcca gcagcaccgt
    31561 agtatgttta tagttttcca tcattttata gagacaagtc ctgcagtcgc tccgataagt
    31621 ctccggtagc caactgctct gcgtcgatat cttccttgac ctgttgatgc caggttgttt
    31681 catcccacag ctcaaacttg ttgaactgtc caaccagcat cacttctttt gtcagcccgg
    31741 catgttgccg cagtactggc gcgattaaca atcgacctgc gccatccatc tgacattcgc
    31801 tggcatgacc taacagtagg cgctgcacac ggcgctcaac cgggttcatg ctcgacagac
    31861 gcgataattt ttgctcgata atttcccatt caggcagggg gtaaagcagc aggcacgggt
    31921 gatgaatgtc aatggtgcaa accatttgac cggcagcgtt ctcaagcagc tgttcccgat
    31981 aacgggtagg cactgataag cgccctttgc tgtcgagatt gactaacgtt gctccccgga
    32041 acatgccagc ctcacccctt attcccactt tgccccacaa tttcccactg aaaggagttt
    32101 acggagcgga ggaaaagctt gtcaagccag tcacaaggca taccgaggta aaaaacccga
    32161 tgtttacggc taatatcagc gttgtttatt ttacgtccaa cgtgcgaagc aaattaacgt
    32221 tatgaatagc tgtaagaaaa aaaccctcat cgtcatcacc gttttaaaat gactcactat
    32281 tttccacagc aaatataaag tgtcagtttg cgacgcgagc ggcattttag gacatatctt
    32341 ccccggttaa cagtccctgt tgcgtctgtg tagcgcttga tgccagcggg tttgcgcaaa
    32401 agcaaagaaa agtgcttgtt aaatcggcaa ttcatctctg catgtaacaa aataatgcaa
    32461 aaacgcgctg tcgatatcac ttaattattg tgaatttcag ctggtgagtt aattaactgg
    32521 cgggtttaaa agcatttatc aataatagtc aggaatagtc ttatttactt taaggatatt
    32581 gatgtccagt cccgtactct acgcgccaga gtgaaattca cctggcgcgt atttttgttc
    32641 gcagcttagc tacggctgag cacaccgcgg cgatagagat tacgtttaat gcgcgttaaa
    32701 ccaggttttg gcttacgcgg ttcgtccagg cttgccagga caatctccag cacacgctct
    32761 gcgacatcgc ggtgacgttg agccactgcc agcaccgggc actgtaagaa gtcgagcagt
    32821 tcgttatcgc caaaggtggc aattgccagg tcagaaggca gtttaccatc gcgacgtagc
    32881 gtgacatcca tcaccccttg caacaacgca aacgacgttg tgaacagcgc ctgcggcatc
    32941 ggatgcgttt ccagccattt ttcgaataac tgggcagccg cctcccgctc atagctgttg
    33001 gcatacagga aatgcacttc gcgcggatca tctttccagg cagtacggaa accttgttca
    33061 cgcaggaagc tgacagaaag ctccggcagc gcgccaagat aaagcaccgt ctcggcggga
    33121 aacttacgta actcttccgc cagcatttcg gcatcatcct gatcggcacc aaccacgctg
    33181 gtaaagtgtt cacgatcgag ggcgcggtcc agcgcgacaa tcgggaacgg gtcgttagcc
    33241 cagcgttgat aaaaaggatg ctcaggaggc aacgacgtcg aaacaataat ggcatcgacc
    33301 tgacgctgta aaaggtgctc aatgcagcgc atttcgttgt ctggctgatc ttctgagcag
    33361 gcaatcagca gttgataacc ccgttgccgc gcctggcgtt caagatagtt agcgatgcgg
    33421 gtatagctgg tgttctccag atcggggatc acaagaccaa tagaacgtgt gcgtccagca
    33481 cgaagcccag ctgccacggc gttcgggtgg taattgtgct cacgcaccac agccatgact
    33541 ttttcaacgg ttttgtcact cacacggtat tgcttcgctt tgccgttaat aacatagctt
    33601 gcagtggtcc gcgacactcc cgccagccga gcgatttcat ccagtttcac aattgcccct
    33661 tgcgtaaaat gtaaaaacca tagccattgt actggtatgg gttaaattca ttaacatcta
    33721 agcgcagaaa attaactgcg gcaactgctt ttattccagg ttcccactga tttcgcaaaa
    33781 aaagcccaac gtgacatgtt gggctgtaaa ttgcgcattg agatcattct caacgcatta
    33841 ttttatcgcc gcgcgaaagt ccgaccacac cagagcgagc aacttccaca attttcgcca
    33901 catcgcgaat cgatgctaaa aatgcatcaa gcttatcgct ggtgcctgct aattgaacgg
    33961 tataaagcga gggtgtgaca tcaataattt gcccacggaa tatttccgta ttacgtttaa
    34021 cttcgtcacg cccgtaaccg ctggcctgaa ttttcaccag catgatttcc ctctcaacat
    34081 gcgcgccctg ccccaactca ctcacgcgca agacatcgac cagcttgtat aattgctttt
    34141 cgatctgctc aagcactttt tcatcgccca cggtctggat ggtcatacgc gataatgtcg
    34201 gatcgtcggt tggcgcaacg gtcaggcttt caatgttgta gccacgctgg gaaaaaaggc
    34261 caatcacgcg ggataacgcg cctgattcat tttcgagtaa gactgataat atccggcgca
    34321 taatcaggtt ctctccgttt tgcttaacca catttcatcc attccgcccc cgcgaatctg
    34381 catcgggtag acgtgctcac tgccatcgac ggtgacatca acaaatacca ggcgattatt
    34441 acgcacctgt tcaagcgcct cgctaagttt gctttccagc tcttgcggat gagagatctg
    34501 gatcccgacg tgaccatagg cttccgccag acggacgaag tctggtaaag actgcatata
    34561 agattgtgaa tgacgaccgg aatagatcat gtcctgccac tgcttcacca tccccaaata
    34621 gcggttatta agattaacca ccagcaccgg cagttcatat tgcaacgctg tagacagttc
    34681 ctgaatgttc atctgaatac tgccgtcgcc agtgacgcaa accacggttt cttctggcag
    34741 cgccattttg acgcccagtg ccgcaggtaa accaaagccc atagtgccga ggccaccgga
    34801 gttgatccaa cgacggggtt tgtcgaatgg ataataaagc gcagcaaaca tctggtgctg
    34861 cccgacatcg gacgtaacgt aagcgccccc cttcgtcaac cgccaaagag tctcgatcac
    34921 cgcctgcggt ttaatctttt cactgtgagt gtcatatttc aggcactgac gagcgcgcca
    34981 ctgttcaatc tgctgccacc agtcgcggat ctcatccagc ggttgatggg cggattcttg
    35041 cgacaagagt tcaagcattt gttcgaggac ctggcgagca tcccccacaa tcggaatatc
    35101 cgcagtcacg gttttagaaa tggaagtagg atcaatatcg atatgcagaa cagtggcatt
    35161 tgggcagtac tttgccagat tgttagtggt gcgatcgtca aagcggacac cgacagcaaa
    35221 aatcacatcc gcgttatgca tcgtcatatt ggcttcgtag gtaccgtgca ttcccagcat
    35281 gcccagtgcc tgacgatgcg ttgccggaaa cgctcccagc cccatcaatg aggaaacaac
    35341 gggcagattt aacgcctcca ccgtttcttt cagctgttga tggcagcccg ccgtgattgc
    35401 cccaccgcct acgtagacaa ccggtttttt tgccgccacc agcgtttgca gagcgcgctt
    35461 aatttgccct ttatgtccgg tagtagtggg attgtaagaa cgcatactga ccgactccgg
    35521 ccagacatag ggtaatttgt tcgccggatt aagaatatct ttcggtaaat caacgaccac
    35581 tggtcctggg cgaccacttg ccgccagcca gaaagccttt ttcagcacct gcggaatgtc
    35641 ttccgtttgc ttaaccagaa aactgtgttt aaccaccggt cgcgaaatcc ccaccatgtc
    35701 gcactcctga aaggcatcgt aacctatcaa cgaggtcgct acctgcccgg aaaggacaac
    35761 taatggaatg gaatccatat aagcggtggc gatgccggta atcgcattgg tcgcccctgg
    35821 acccgacgtt accagcacga cgccgacttc cccggtcgcg cgcgccagac catcggccat
    35881 atgcactgcc gcctgctcat gacgaactaa aacatgaccg ataccaccca cggtatgcaa
    35941 tgcatcgtaa atatcaagga ctgcgcctcc gggataaccg aatacttgtt taacgccctg
    36001 atcgataagc gatcggacga ccatctcggc tccagacaac atctccatgg cctgcctcac
    36061 tgtttgacgg aaaaaatgtg taaaacacat taaccgtccg atcttgctta agcaattggc
    36121 agccagagac ataaacgccg ggtaataaga ataaacagga ggaaaggtga aaagaaacga
    36181 taaaatcctc cattacgaag gataaaaatg ataataaatc ggctaaatcc caccacttac
    36241 agcaatttct ctattttttt aattaatcct aaaatagaga gcccacacat tcagaataaa
    36301 attctacact caataaaaaa tgcagaataa accagacatt catgtctgac ctattctgca
    36361 ttcagttagc gtttgcaaat tgagactaat tgctcttcca tccactgatg gcctttatca
    36421 cgcccggcag cttcatgcca ggagagataa caggttctgc tatttagttt taacggcagc
    36481 ggtaatacct gtaattctaa ggattcagcg aactcttcag ccagccaacg cggcgcaata
    36541 gcgaccaaat gcgtttgcga caccacgcta agtacgctca tcattgccat gccctgatac
    36601 gcgatactgg cttgcttatc taccgtgtca taccaaggtt gactaaatga cgcgaaacga
    36661 tcgagcgaaa ccgccgcatg ttgttcgtta taaacatcat gtttcagtaa cgggccctta
    36721 attgttgggt gatttttgct ggctaccagc accatttcat ctttaaataa tggcacgctg
    36781 gtaaattcag gacgatggaa gtcttcataa ctaatcacaa actccgtttc ctgataacgc
    36841 agctgatgtt cagtgttctg atttaatgaa gacttgaaca taacatgtat atttggcgca
    36901 atctgctcaa tgtgattata aatctgcgag gtcagaatac tgtctaacgg gctgcaaaca
    36961 caaagatgaa atacacgttc actgctcgcg ggttcaaaac ctgaaccagg caattcattt
    37021 tgtactagtt gcaatgcctg acgaactgaa ccaaaaagtt gaaatgcgcg agcagtcggt
    37081 tgaataccac ggccataacg aacaaaaagc tcgtcattaa acatcacctt caggcgtgca
    37141 acagcgttac tgaccgcagg ttgcgacatt cccagaacat gagcagcacg ggtaatgttt
    37201 tgctcctgca tcacggcatc gaaaacggtt aataagttga gatcgaccat gcgtagctgt
    37261 ggcttgctga actccgccgt ctctggatga tctgtttgta cctctggcat acttaactcc
    37321 actgtcacac ttaactccct ttcccttatt ggaatgcaga gaaataatcc tcgagaatat
    37381 gatttatcat gcatataaaa taagaaaaag gaaaatgagt aaaattagga aaacataaag
    37441 atacgggttc ttataaaaac agaatccatt gccgtggaaa gcgtcattta ccac
//
LOCUS       DAZFAB010000037        34599 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZFAB010000037 DAZFAB010000000
VERSION     DAZFAB010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34599
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.037"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            2..637
                     /locus_tag="ACHJWA_002168"
     CDS             2..637
                     /locus_tag="ACHJWA_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4308765.1"
                     /translation="MAVNHFRYKFLLSGNAGEMILYFANVFFNPLALSFIIATIICIT
                     IKKRSSRSFIRGTCWLMGLFLIYSSYTVWERHSIWDYTFPEPGITVTVPSKQWVANIV
                     KQGPTLVTRDAHAILAIVAFSQNELGVHSIEELTATQNGTAEMHVCDVQGFACAYQEG
                     VQTMSDGKVRHAIFMTLLDNTNLIQLVAAIDPDYLDEYQEEVMKMMLSARQ"
     gene            760..1386
                     /locus_tag="ACHJWA_002169"
     CDS             760..1386
                     /locus_tag="ACHJWA_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4308766.1"
                     /translation="MVGEYIGSVLLGPLLLPVLVSGILCTFTKKTRNFASFTRGCCWV
                     LGVLLLSNVGNTFRLFTPWHYTFEKAAISVTVPNRHWNTVSISTDKTIDIRSEDNSVF
                     ISAFRLPAGRSADDSLEELKKMQRDNLKDQYNEETFQFHDCNAKHFTCKYQDVLINFD
                     GQQKRTISVYLEDTPRAVGIIALMEPDTADKYRQQAMEIMLSAKNTVK"
     gene            complement(1425..1670)
                     /gene="zapB"
                     /locus_tag="ACHJWA_002170"
     CDS             complement(1425..1670)
                     /gene="zapB"
                     /locus_tag="ACHJWA_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFO4308767.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            2096..2941
                     /gene="glpF"
                     /locus_tag="ACHJWA_002171"
     CDS             2096..2941
                     /gene="glpF"
                     /locus_tag="ACHJWA_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFO4308768.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            2964..4472
                     /gene="glpK"
                     /locus_tag="ACHJWA_002172"
     CDS             2964..4472
                     /gene="glpK"
                     /locus_tag="ACHJWA_002172"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFO4308769.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            4702..5712
                     /gene="glpX"
                     /locus_tag="ACHJWA_002173"
     CDS             4702..5712
                     /gene="glpX"
                     /locus_tag="ACHJWA_002173"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFO4308770.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANSLA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIESGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            5809..6555
                     /gene="fpr"
                     /locus_tag="ACHJWA_002174"
     CDS             5809..6555
                     /gene="fpr"
                     /locus_tag="ACHJWA_002174"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFO4308771.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVHPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(6560..6988)
                     /gene="uspD"
                     /locus_tag="ACHJWA_002175"
     CDS             complement(6560..6988)
                     /gene="uspD"
                     /locus_tag="ACHJWA_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFO4308772.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKLSADLLIVPFIDK"
     gene            complement(7015..7314)
                     /gene="yiiS"
                     /locus_tag="ACHJWA_002176"
     CDS             complement(7015..7314)
                     /gene="yiiS"
                     /locus_tag="ACHJWA_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFO4308773.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(7526..7966)
                     /gene="yiiR"
                     /locus_tag="ACHJWA_002177"
     CDS             complement(7526..7966)
                     /gene="yiiR"
                     /locus_tag="ACHJWA_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO4308774.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            8067..8666
                     /gene="yiiQ"
                     /locus_tag="ACHJWA_002178"
     CDS             8067..8666
                     /gene="yiiQ"
                     /locus_tag="ACHJWA_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFO4308775.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            8774..9541
                     /gene="tpiA"
                     /locus_tag="ACHJWA_002179"
     CDS             8774..9541
                     /gene="tpiA"
                     /locus_tag="ACHJWA_002179"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFO4308776.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(9596..10351)
                     /gene="cdh"
                     /locus_tag="ACHJWA_002180"
     CDS             complement(9596..10351)
                     /gene="cdh"
                     /locus_tag="ACHJWA_002180"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFO4308777.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(10458..11447)
                     /gene="sbp"
                     /locus_tag="ACHJWA_002181"
     CDS             complement(10458..11447)
                     /gene="sbp"
                     /locus_tag="ACHJWA_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFO4308778.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKLGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLISWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(11766..12728)
                     /gene="pfkA"
                     /locus_tag="ACHJWA_002182"
     CDS             complement(11766..12728)
                     /gene="pfkA"
                     /locus_tag="ACHJWA_002182"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFO4308779.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(12909..13811)
                     /gene="fieF"
                     /locus_tag="ACHJWA_002183"
     CDS             complement(12909..13811)
                     /gene="fieF"
                     /locus_tag="ACHJWA_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFO4308780.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(13960..14460)
                     /gene="cpxP"
                     /locus_tag="ACHJWA_002184"
     CDS             complement(13960..14460)
                     /gene="cpxP"
                     /locus_tag="ACHJWA_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFO4308781.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            14610..15308
                     /gene="cpxR"
                     /locus_tag="ACHJWA_002185"
     CDS             14610..15308
                     /gene="cpxR"
                     /locus_tag="ACHJWA_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFO4308782.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            15305..16678
                     /gene="cpxA"
                     /locus_tag="ACHJWA_002186"
     CDS             15305..16678
                     /gene="cpxA"
                     /locus_tag="ACHJWA_002186"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFO4308783.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(16784..17458)
                     /gene="yiiM"
                     /locus_tag="ACHJWA_002187"
     CDS             complement(16784..17458)
                     /gene="yiiM"
                     /locus_tag="ACHJWA_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFO4308784.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYIGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(17607..18590)
                     /gene="kdgT"
                     /locus_tag="ACHJWA_002188"
     CDS             complement(17607..18590)
                     /gene="kdgT"
                     /locus_tag="ACHJWA_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFO4308785.1"
                     /translation="MQIKRLIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(18850..19470)
                     /gene="sodA"
                     /locus_tag="ACHJWA_002189"
     CDS             complement(18850..19470)
                     /gene="sodA"
                     /locus_tag="ACHJWA_002189"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFO4308786.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            19755..20789
                     /gene="rhaT"
                     /locus_tag="ACHJWA_002190"
                     /pseudo
     CDS             19755..20789
                     /gene="rhaT"
                     /locus_tag="ACHJWA_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(20786..21634)
                     /gene="rhaR"
                     /locus_tag="ACHJWA_002191"
     CDS             complement(20786..21634)
                     /gene="rhaR"
                     /locus_tag="ACHJWA_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFO4308787.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(21708..22544)
                     /gene="rhaS"
                     /locus_tag="ACHJWA_002192"
     CDS             complement(21708..22544)
                     /gene="rhaS"
                     /locus_tag="ACHJWA_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFO4308788.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYHCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            22832..24301
                     /gene="rhaB"
                     /locus_tag="ACHJWA_002193"
     CDS             22832..24301
                     /gene="rhaB"
                     /locus_tag="ACHJWA_002193"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFO4308789.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALISATQALPACRFIINP
                     NDDRFINPETMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVARIHSTRQTKELCA"
     gene            24298..25557
                     /gene="rhaA"
                     /locus_tag="ACHJWA_002194"
     CDS             24298..25557
                     /gene="rhaA"
                     /locus_tag="ACHJWA_002194"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFO4308790.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWIIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKETLSRRG"
     gene            25732..26556
                     /gene="rhaD"
                     /locus_tag="ACHJWA_002195"
     CDS             25732..26556
                     /gene="rhaD"
                     /locus_tag="ACHJWA_002195"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFO4308791.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAL
                     YHDNFHPQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDETFGLIDTAEKSAEVLVKVYSMGGMKQTISREELIALGQRFGVTPLASALAL"
     gene            26566..26880
                     /gene="rhaM"
                     /locus_tag="ACHJWA_002196"
     CDS             26566..26880
                     /gene="rhaM"
                     /locus_tag="ACHJWA_002196"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFO4308792.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTEICQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            complement(26921..28315)
                     /locus_tag="ACHJWA_002197"
     CDS             complement(26921..28315)
                     /locus_tag="ACHJWA_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFO4308793.1"
                     /translation="MKKSTLSLAIGLLLACSTGMAKTQHLMLEQRMALLEERLEAAEM
                     RAAKAESQVKQLQTQQAAEIREIKAAQGNTPVNGQATAESAKKNATSPNLLLSGYGDL
                     KIYGDVEFNMDAESNHGLLAMTNADVNSDPTNEQWNLNGRILLGFDGMRKMDNGYFAG
                     FSAQPLGDMHGSVNIDDAVFFFGKENDWKVKVGRFEAYDMFPLNQDTFVEHSGNTAND
                     LYDDGSGYIYMMKEGRGRSNAGGNFLVSKQLDNWYFELNTLLEDGTSLYNDGNYHGRD
                     MEQQKNVAYLRPVIAWSPTEEFTVSAAMEANVVNNAYGYTDSKGNFVDQSDRTGYGMS
                     MTWNGLKTDPDNGIVVNLNTAYLDANNEKDFTAGINALWKRFELGYIYAHNKIDEFSG
                     VVCDNDCWIDDEGTYTIHTIHASYQFANVMDMENFNIYLGTYYSILDSDGDKKHGDDT
                     DDRYGARVRFKYFF"
     gene            28793..29239
                     /gene="frvA"
                     /locus_tag="ACHJWA_002198"
     CDS             28793..29239
                     /gene="frvA"
                     /locus_tag="ACHJWA_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFO4308794.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATMALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSTCVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQEFIHQLQQGDTDQVLALLNQTLSS"
     gene            29250..30689
                     /gene="frvB"
                     /locus_tag="ACHJWA_002199"
     CDS             29250..30689
                     /gene="frvB"
                     /locus_tag="ACHJWA_002199"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HFO4308795.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAIPYAL
                     AAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLAIGLA
                     LNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            30679..31749
                     /gene="frvX"
                     /locus_tag="ACHJWA_002200"
     CDS             30679..31749
                     /gene="frvX"
                     /locus_tag="ACHJWA_002200"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO4308796.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSREEAKKRGAEIGDFISPEAN
                     FACWGEDKIVGKALDNRIGCAMMAELLQTVNNPGITLYGVGSVEEEVGLRGAQTSAEH
                     TKPDVVIVLDTAVAGDVPGIDNIKYPLKLGNGPGLMLFDKRYLPNQKLVAALKNCAAH
                     NGLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            31749..33497
                     /gene="frvR"
                     /locus_tag="ACHJWA_002201"
     CDS             31749..33497
                     /gene="frvR"
                     /locus_tag="ACHJWA_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HFO4308797.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARISASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EHNGLFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGLASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(33547..>34599)
                     /gene="yiiG"
                     /locus_tag="ACHJWA_002202"
     CDS             complement(33547..>34599)
                     /gene="yiiG"
                     /locus_tag="ACHJWA_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HFO4308798.1"
                     /translation="KRNLLSSAIIVAIMALGLTGCDDKKAETETPLPANSQPAAPAPE
                     AKPTEAPVAKADAKPETPAQPLVDEQAVFDEKMDVYIKCYNKLQIPVQRSLARYADWL
                     KDFKQGPTGKESTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVA
                     LGNTINEMDKYYTQENYKDDAFAKGKTLHQTFLKNLEAFEPVAESYHAAIQEINDKRQ
                     LAELKNIEEREGKTFHYYSLAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQAK
                     EADKGGMNFSFINSADQYQLETKKYVRRVRDKVPYSDWDKEHLQDANTSWMVEDSFPR
                     ALREYNEMVDDYNSLR"
BASE COUNT         8547 a         8832 c         8625 g         8595 t
ORIGIN      
        1 tatagccgtt aaccatttcc gctacaaatt tcttttatct ggcaacgcgg gtgaaatgat
       61 tttatatttt gctaacgtat tttttaaccc acttgcctta tcatttatta tcgccacaat
      121 catctgtata acgataaaaa aaagaagcag ccgcagcttt attcgcggca cttgttggtt
      181 aatgggatta tttcttatct atagcagcta tacggtgtgg gaacgacaca gcatatggga
      241 ttatactttc ccggagccag gtatcaccgt cacggttccg agcaaacagt gggttgccaa
      301 tatagtaaaa caaggaccaa ccctggtgac ccgggatgcc catgctattt tagctattgt
      361 tgccttctca caaaatgaac tcggtgtgca ttcaatcgag gaacttaccg cgactcaaaa
      421 cggaactgca gaaatgcatg tttgtgatgt tcagggattt gcctgcgctt atcaggaagg
      481 agtgcaaaca atgagtgatg gcaaagtaag acatgctatt ttcatgactc tactggataa
      541 cactaatttg atacaactgg tcgcagctat cgacccagat tatttggacg aatatcaaga
      601 agaagtaatg aaaatgatgc tttctgctcg tcaatgatct gatgtgcatg ttaataataa
      661 tggattgata tgagatacgt tatcttatta atactatgga taataactgt ttttcttagt
      721 catcttcagc acagtaactt gcttactgga gatccaggga tggttggcga gtatatcggt
      781 tcagtacttc ttggaccgtt attacttccc gtgcttgtgt caggtattct gtgcacattc
      841 actaaaaaaa cgcgtaactt cgccagtttt actcgcgggt gctgttgggt attgggagtc
      901 ctgcttttat ccaacgtcgg caatacgttt cgtctgttca ccccgtggca ctatacgttt
      961 gagaaggcag ctatttccgt gactgtccca aaccgacact ggaatacggt ttctattagt
     1021 actgataaaa ccattgatat aagaagtgaa gataatagcg tctttatttc tgctttccgc
     1081 ctgcccgcag gacgtagcgc cgatgactcg ctggaagagt taaagaagat gcagcgagac
     1141 aatctcaagg atcaatacaa tgaagaaacc ttccagtttc atgattgtaa tgccaaacat
     1201 ttcacctgta agtatcagga tgtgttgata aattttgatg gtcagcagaa aagaaccatc
     1261 agtgtttatc ttgaagacac ccccagggct gtggggatca ttgcattaat ggagccagat
     1321 acggcagata aatatcgcca acaggcgatg gagattatgt tgtctgctaa aaatacagtg
     1381 aagtaaaaaa taaggcgcct tgcggcgcct ctgcgtggaa gagattaaac ctcttccatg
     1441 cgacccagca gggcctgcag acgttcctgc cagccgttct gctgttcttt cagatggttg
     1501 ttctcacgct ccagctcttc gcgctgatgc tgggcatttt gaacttcctg cgacaatgag
     1561 ttgttttttt ctttcagctc ttcgatttcc atctgcaaca gagtgatggt atcaatcgcc
     1621 tgctgtactt ttgcttccag tttctcaaac acttctaatg acattgtcat acctctcctg
     1681 aattgcaagg cgttgatgga taaaaaatcc tcgtcccgat taccggtgac gccttaataa
     1741 atacgagcgc actttagtta gctccgattg tatgaagccg cgccatcgct gtccagcggc
     1801 acgccttgca gattacggtt tgccacactt ttcatccttc tcctggtgac ataatccaca
     1861 ccaatcgaaa atgttaataa atttgttgcg cgaatgttct aacaaacatg catcatgtac
     1921 aatcagatgg aataaatggc gcgataacgc tcattttatg acgaagcaca cacattttaa
     1981 gttcgatatt tctcgttttt gctcgttaac gataagttta cagcatgcct acaagcatcg
     2041 tggaggtccg tgactttcac gcatacaaca aacattaact cttcaggatc cgattatgag
     2101 tcaaacatca accttgaaag gccagtgcat tgctgaattc ctcggtaccg ggttgttgat
     2161 ttttttcggt gtgggttgcg ttgcagcact aaaagtcgct ggtgcgtctt ttggtcagtg
     2221 ggaaatcagt gtcatttggg gactgggggt ggcaatggcc atctacctga ccgcaggggt
     2281 ttccggcgcg catcttaatc ccgctgttac cattgcattg tggctgtttg cctgtttcga
     2341 caagcgcaaa gttattcctt ttatcgtttc acaagttgcc ggcgctttct gtgctgcggc
     2401 tttagtttac gggctttact acaatttatt tttcgacttc gagcagactc atcacattgt
     2461 tcgcggcagc gttgaaagtg ttgatctggc aggcactttc tctacttacc ctaatcctca
     2521 tatcaatttt gtgcaggctt tcgcagttga gatggtgatt accgctattc tgatggggct
     2581 gatcctggcg ttaacggacg atggcaacgg tgtaccacgc ggccctttgg ctcccttgct
     2641 gattggtcta ctgattgcgg tcattggcgc atctatgggc ccattgacgg gttttgccat
     2701 gaacccagcg cgtgacttcg gtccgaaagt ctttgcctgg ctggcgggct ggggcaatgt
     2761 cgcctttacc ggcggcagag acattcctta cttcctggtg ccgctttttg gccctatcgt
     2821 tggcgcgatt gtaggtgcat ttgcctaccg caaactgatt ggtcgccatt tgccttgcga
     2881 tatctgtgtt gtggaagaaa aggagaccac aactccttca gaacaaaaag cttcgctgta
     2941 atatgactac gggacaatta aacatgactg aaaaaaaata tatcgttgcg ctcgaccagg
     3001 gcaccaccag ctcccgcgcg gtcgtaatgg atcacgatgc caatatcatt agcgtgtcgc
     3061 agcgcgaatt tgagcaaatc tacccaaaac caggctgggt agaacacgac ccaatggaaa
     3121 tctgggccac ccaaagctcc acgctggtag aagtgctggc gaaagccgat atcagttccg
     3181 atcaaattgc agctatcggt attacgaacc agcgtgaaac cactattgtc tgggaaaaag
     3241 aaaccggcaa gcctatctat aacgccattg tctggcagtg ccgtcgtacc gcagaaatct
     3301 gcgagcattt aaaacgtgac ggtttagaag attatatccg cagcaatacc ggtctggtga
     3361 ttgacccgta cttttctggc accaaagtga agtggatcct cgaccatgtg gaaggctctc
     3421 gcgagcgtgc gcgtcgtggt gaattgctgt ttggtacggt tgatacgtgg cttatctgga
     3481 aaatgactca gggccgtgtc catgtgaccg attacaccaa cgcctctcgt accatgttgt
     3541 tcaacatcca taccctggac tgggacgaca aaatgctgga agtgctggat attccgcgcg
     3601 agatgctgcc agaagtgcgt cgttcttccg aagtgtacgg tcagactaac attggcggca
     3661 aaggcggcac gcgtattcca atctccggga tcgccggtga ccagcaggcc gcgctgtttg
     3721 gtcagttgtg cgtgaaagaa gggatggcga agaacaccta tggcactggc tgctttatgc
     3781 tgatgaacac tggcgagaaa gcggtgaaat cagaaaacgg cctactgacc accatcgcct
     3841 gcggcccgac tggcgaagtg aactatgcgt tggaaggtgc ggtgtttatg gcaggcgcat
     3901 ccattcagtg gctgcgcgat gagatgaagt tgattaacga cgcctacgat tccgaatatt
     3961 tcgccaccaa agtgcaaaac accaatggtg tgtatgtggt tccggcattt accgggctgg
     4021 gtgcgccgta ctgggacccg tatgcgcgcg gggcgatttt cggtctgact cgtggggtga
     4081 acgctaacca cattatacgc gcgacgctgg agtctattgc ttatcagacg cgtgacgtgc
     4141 tggaagcgat gcaggccgac tctggtatcc gtctgcacgc cctgcgcgtg gatggtggcg
     4201 cagtagcaaa caatttcctg atgcagttcc agtctgatat tctcggcacc cgcgttgagc
     4261 gcccggaagt tcgcgaagtc accgcattgg gtgcggccta tctcgcaggc ctggcggttg
     4321 gcttctggca gaacctcgac gagctgcaag agaaagcggt gattgagcgc gagttccgtc
     4381 caggcatcga aaccactgag cgtaattacc gttacgcagg ctggaaaaaa gcggtcaaac
     4441 gcgcgatggc gtgggaagaa cacgacgagt aatgtcaggc acctgaatcg attgcctgat
     4501 gctgatcgcc tgatgcgacg cttacgcgtc ttatcaggcc tacgagccaa tgccattttg
     4561 tgtaggcggg ataaggcgtt cacgccgcat ccggcatcac gcagactctg aaaccgtgcg
     4621 ggggcaaccc cgcacacatc aataatccca cccttcccct gtgctacact tcgcgccatt
     4681 ccttactgct tagagtttgc tatgagacga gaacttgcca tcgaattttc ccgcgtcacc
     4741 gaatctgcgg cgctggctgg ctacaaatgg ttaggacgcg gcgataaaaa caccgcggac
     4801 ggcgcggcgg taaacgccat gcgtattatg ctcaaccagg tcaacattga cggcaccatc
     4861 gtcattggtg aaggtgaaat cgacgaagca ccgatgctct acattggtga aaaagtcggt
     4921 actggtcgcg gcgacgcggt agatattgct gttgatccga ttgaaggcac gcgcatgacg
     4981 gcgatgggtc aggctaactc gctggcggtg ctggcagtag gcgataaagg ctgtttcctc
     5041 aacgcgccag atatgtatat ggagaagctg atcgtcggac cgggagccaa aggcaccatt
     5101 gatctgaacc tgccgctggc ggataacctg cgcaatgtcg cggcggcgct cggcaaaccg
     5161 ttgagcgaac tgacggtaac gattctggct aaaccacgcc acgatgccgt tatcgctgaa
     5221 atgcagcaac tcggcgtacg cgtatttgct attccggatg gcgacgttgc ggcctcaatt
     5281 ctcacctgta tgccagacag cgaagttgac gtgctgtacg gaattggtgg cgcgccggaa
     5341 ggcgtggttt ctgcggcggt gatccgcgca ttagatggcg acatgaacgg tcgtctgctg
     5401 gcgcgtcatg acgtcaaagg cgacaacgaa gagaatcgtc gcattggcga gcaggagctg
     5461 gcacgctgca aagcgatggg catcgaatcc ggtaaagtat tgcgtctggg cgatatggcg
     5521 cgcagcgata acgtcatctt ctctgccacc ggtattacca aaggcgatct gctggaaggc
     5581 attagccgca aaggcaatat cgcgactacc gaaacgctgc tgatccgcgg caaatcacgc
     5641 accattcgcc gcattcagtc tatccactat ctggatcgca aagacccgga aatgcaggtg
     5701 cacatcctct gattgatttg atcgattgag ccttccagtc cttcgggact ggaatttttt
     5761 tgttcggaga acgaagataa ggcaattcaa tcaaaacagg agaaaaacat ggctgattgg
     5821 gtaacaggca aagtcactaa agtgcagaac tggaccgacg ccctgtttag tctcaccgtt
     5881 cacgcccctg tgcatccgtt taccgccggg caatttacca agcttggcct tgaaatcgac
     5941 ggcgaacgcg tccaacgcgc ctactcctat gttaactcgc ccgataatcc cgatctggag
     6001 ttttacctgg tcaccgtccc cgatggcaaa ttaagcccac gattggcggc actgaaaccg
     6061 ggcgatgaag tgcaggtggt tagcgaagca gctggcttct ttgttctgga tgaagtacca
     6121 gattgcgaaa cgctatggat gctggcaacc ggtacagcga ttggccctta tttatcgatt
     6181 ctgcaactag gtaaagattt agatcgcttc aaaaatctgg tcctggttca cgccgcacgt
     6241 tatgccgccg acttaagcta tttgccactg atgcaagaac tggaaaaacg ctacgaaggc
     6301 aaactgcgca ttcagacggt ggtcagtcgg gaaacggcag cggggtcgct caccggacgg
     6361 ataccggcat taattgaaag tggtgaactg gaaagcgcga ttggcctgcc gatgaataaa
     6421 gaaaccagcc atgtgatgct gtgcggcaat ccacagatgg tgcgcgatac gcaacagttg
     6481 ctgaaagaga cccggcagat gacgaaacat ttacgtcgcc gaccgggtca catgacagcg
     6541 gagcattact ggtaagcggt tacttatcga taaacggcac gatgagcaaa tccgcactca
     6601 acttattgat catcccgcga tatgccggca tcaaacggtt gataaatgag tggtgatgac
     6661 cacagacaag gaggtcgcac tgctcttttt gcataatttc cagcagtgtt tccggcattt
     6721 ctccgcgttc aatacgcagt tttgtcttcg gccattgaat atttttcgtc agtttataca
     6781 gcttgttatc cgacttattc ttcaacaatt gaagaatatc ttctgttgca gggaagtaga
     6841 tacccggata caactcgctt aagccatcat caatatgaat taacgtcagg tgagcgtcat
     6901 tatgtctggc gagctccaag gctttattca ccagtaaggc atcttcttca ttcccggaaa
     6961 ttgccacgcc aatgtgttta taagccatgt ttaactcctt ctaaagcaac tccatcaagc
     7021 taacgaacgc agtgataact caaaaataat catctcggcc tggcatgaga aggtgaaggc
     7081 cgcatcaagc tcaacttgcc cctccagatc cttaatctta taggccacgc tggccccttc
     7141 atctgcagag gatgcagcgg cggcaagctc tttcagtttg gtcataaagg actctgcttc
     7201 ttcgcgggtg gcaaaaaagc gcgaaaactt actggtacag ttgtcgttat caatcaccgt
     7261 accgatatca atcgcacatc ctttagtact gcatttatct acgacatctt tcatagggca
     7321 cctctgtata ttcgcctctc tgtcgattac gacttaagaa taatgcgccg ttaagggaag
     7381 aacaaacaag taatgatctt atggattacc attttttttg atattgcgta aggtgaagag
     7441 ttcagaagga ttttccgggt attgtcattt gcatcgtgga aataacaatc ccggaaaaaa
     7501 atagcagtaa gagatatatt aaaggttaat tacttgattt attgtcggct ttatacttca
     7561 catcctgagt atctttacca tatttatttt cgccttgtgt gccaacaaac gcaccaagat
     7621 cgataagcat catcaccaga atcaacgtcg ggacaaaacg ccctaccgcc cattgccaga
     7681 cacccggtaa aatcgcccag ttacccgcca gtagcatcca cgccacaatc atcagaaatg
     7741 cccatgcgcc ggaacgcccg cgatcatgca agcgcttaac ggttactgcc gctgttggcc
     7801 agagcaagca cacaaggcaa aacgccgcgg tctgaatatc gagtaaattt ttacccgcca
     7861 gtgagaaaag caccagcatg cctgcgaacc acaggcctat ccaaatccag aaatcacggc
     7921 gtccaatacg ccctttaaat gagaataacc attgctgtat ggtcatgtaa gttccttgat
     7981 ggttgtcttt tccaggattc tacccttttg acaagggcga cagatatcgt tttaatcgga
     8041 gccagtcata acaaaaggta ctgtcaatga agccagggtg tacgctgttt tttctcttat
     8101 gttctgcatt aaccgttaca acaacggcgc atgcacaaac accagatacg gcaacgaccg
     8161 cgccttatct gctggctgga gcccctactt tcgatctctc catcagccag tttcgagaag
     8221 actttaacag ccagaatccc agcctgccac tgaacgaatt tcgtgccatc gacagcagtc
     8281 ccgacaaagc caatctcact cgtgctgcca gtaaaattaa cgagaacttg tatgcttcta
     8341 cagcgctgga gcgcggtacc ttaaaaatca aaagcattca aatgacatgg ctacccatcc
     8401 aggggccaga gcaaaaagcc gcgaaagcga aagctcagga atacatggca gcggtgatcc
     8461 gcacactcac cccattaatg accaaaacac aaagccagaa aaaactgcag tcgctactaa
     8521 cggcggggaa aaacaaacgt tattacaccg agacagaagg tgcactgcgc tatgttgtcg
     8581 cggacaacgg cgaaaagggg ctgaccttcg ctgttgaacc gattaagctg gcgctatctg
     8641 aatcgcttga aggtttgaat aaatgacaaa aagcaaagcc tttgtgccga tgaatctcta
     8701 tactgtttca cagacctgct gccctgcggg gcggccatct tcctttattc gcttataagc
     8761 gtggagaatt aaaatgcgac atcctttagt gatgggtaac tggaaactga acggcagccg
     8821 ccacatggtt cacgagctgg tttctaacct gcgtaaagag ctggcaggtg ttgctggctg
     8881 tgcggttgca atcgcaccac cggaaatgta catcgatatg gcgaagcgtg aagctgaagg
     8941 cagccacatc atgctgggtg cgcaaaacgt ggacctgaac ctgtccggcg cattcaccgg
     9001 tgaaacctct gctgctatgc tgaaagacat cggcgcacag tacatcatca tcggtcactc
     9061 tgaacgtcgt acttaccaca aagaatctga cgaactgatc gcgaaaaaat tcgcggtgct
     9121 gaaagagcag ggcctgactc cggttctgtg catcggtgaa accgaagctg aaaacgaagc
     9181 gggcaaaact gaagaagttt gcgcacgtca gatcgacgcg gtactgaaaa ctcagggtgc
     9241 tgcggcattc gaaggtgcgg ttatcgctta cgaacctgta tgggcaatcg gtactggcaa
     9301 atctgcaact ccggctcagg cacaggctgt tcacaaattc attcgtgacc acatcgctaa
     9361 agttgacgct aacatcgctg aacaagtgat cattcagtac ggcggctctg taaacgcgtc
     9421 taacgctgca gaactgtttg ctcagccaga catcgacggc gcgctggttg gcggtgcgtc
     9481 tctgaaagct gacgctttcg cagtaatcgt taaagctgca gaagcggcta aacaggctta
     9541 agtctgacag gtgccggatt tcatatccgg cacttacttt ccttaactct tcgccttaac
     9601 gcaaaatctc acactgatga tcctgaattt cctcggctga agcacggtta agcgtcagta
     9661 gatttcgttg tgtcgccagc aatacaaatg agttatcact ctgccgtacc atcgccagcc
     9721 cgtagcttcc catatgttcc cgcgcctcag gtacttcttc tgccagcatc ataaatgggc
     9781 tgcgttgtac cagttcgctt tccgttaccc gacgcgccag gtattcatgc ccgcgcaaac
     9841 cacctggcag tggcaaccag cggctgctga tgttcgccag attgttatcc agctgttcgc
     9901 gcacatcagg acgaatacaa gagatatgaa tatgaaaatg gttttgcgta cgcccggtgc
     9961 gggagttgat cgccaaagaa accgcgcgat cgggaaccgg ctggccgtat tttttgctca
    10021 taaaatcacg cgcctgccag gccaaccaaa agaagttcgg cgttgaagga tcggtcaaca
    10081 aaggactttc agtaccgtta atacgatacg ttggcatcaa cagatattgc agtgggccat
    10141 taagatcttt taaaaccacg tatccggcat tgggtttgac ttccgcacat ggcgaaggat
    10201 tttgattttg ctgctgattg ggcaaacatt cctcaaggac aatcttacgt aatgtatccg
    10261 actcttcacc ggttaatttc cagtaaccaa taccggcagc cacaacggcg ataactatca
    10321 tcaccaaaaa aagaagaccc gcttttttca tctttttttc cctgtacctc aaagagatgc
    10381 aagggtaacg caaaatcgtg acaaataaaa aacccggtcg cgagaaacca ccgggtttga
    10441 taattatcct ggggaaatca gcgtttgctg atctgatcga acgtaccgcc gttagcaaaa
    10501 tgctcttttt gcgctttcgt ccagccgccg aactcttcat caatggtgaa taacttcagc
    10561 tttggaaacg cattttcgta ctttttcgcc acctcagcgt cgcgcggacg gtagtagttt
    10621 ttcgcggcaa tttcctgacc ttctggcgag tagagatatt tcaggtaggc ttccgccacc
    10681 tctttagtac cttttttctc gaccacttta tcgaccaccg acacggttgg ctctgcgagg
    10741 atagactcac tcggcgtgac gatttcgaat ttatctttcc ccagttcatt cgctgccagc
    10801 agagcttcgt tttcccagga aatcagtaca tcgccaattc cgcgctcgac aaaagtgtta
    10861 gtggagccgc gcgcgccaga atccagaact tcgacgtttt tatacagtgc ccgaacaaaa
    10921 tcctgtgctt ttgcctgatc gttgttgttg tgatgcagcg cgtagcccca ggctgccagg
    10981 tagttccagc gcgcgccacc agagcttttc ggattaggcg tgatcaccga aacacccagt
    11041 ttaatcagat cgttccagtc atggatctgc ttcggatttc ccttacgtac caggaaaaca
    11101 atggtggaag tgtacggtgc ggagttatcc ggcagacgtt tgatccactc tttatcaatc
    11161 cgcccgcgtt ccgcaatcgc gtccacgtca taggccagag ccagcgtgac aacatcagct
    11221 tcaataccgt tgattaccga cgttgcttgt ttgcctgaac caccgtgtga ctgacgaatc
    11281 accacgttat cgccagtttg ctgtttccag tgggcgctga atgccttgtt gtactgttcg
    11341 tacaattcgc gcgttggatc atatgaaacg ttaagaagct gaatatcctt tgccataacg
    11401 ctggttgccg ccagcaaaaa tgttaaccct acgccccact tgttcatcgc ccgactctct
    11461 tatgttgtgt tgtgatgagc aaagcgtgcc agaaggttaa ccaaacatta aagaataaaa
    11521 aaagattggc tataacttgc gggtatatgt tgggggttaa aaaggcggaa gaaatccgcc
    11581 tcatattgct gacaaagtgc gctttatcga tgccggatgc ggcgcgaacg ccttatccgg
    11641 cctacaaaag tttgtaaatt caataaattg tagaattcac gtaggcctga taagcgaagc
    11701 gcatcaggca tttttgcttc tgtcatcggt ttcaggctaa aggaatctgc ctttttccga
    11761 aatcattagt acagtttttt cgcgcagtcc agccagtcgc ctttgaacgg acgcttcatg
    11821 ttttcgatag cgtcgatgat gtcgtggtga accagctgtt cgttctgtat acctacgcaa
    11881 cgaccgccgt aacctgccag cagcagatcg atagcgtaag cgcccatacg ggaagccaga
    11941 atacggtcgt aaggcaccgg agaaccaccg cgctggatgt ggcccagcac agttgcgcgg
    12001 gtttcacgac cggtttcttt ctcgatgaaa tgcgccagtt cgtcaacatc acacatatgt
    12061 tcggtaatcg ccacgatcgc gtgtttttta cctttcgcga tacccgcttt gatttcgttt
    12121 accaggtctt cacggctgaa ttcaacttcc ggaaccacaa cgaattcaca gccaccggca
    12181 atggccgcag ccagcgtcag atcgccacaa taacggccca tcacttccac cacggaaata
    12241 cgctggtgag aagaagaggt gtcacgcaga cggtcgatcg cttctacaac ggtgctcagc
    12301 gcagtgaaga aaccgatagt gtagtcagtg cctttgatgt cgttgtcgat agtgcccggc
    12361 aggccgatgc acgggaagcc catttcggtc agacgcattg cacccatgta ggaaccgtca
    12421 ccgccgataa ccaccagcgc atcgatccca cgttttttca ggttttcgat agccacggcg
    12481 cggatgttct cgtcgcggaa ttccgggaaa cgcgcagaac cgaggaacgt accgccacgg
    12541 ttgatcatgt cagaaacgct gtaacggtct agctgtacca tacggtcttc atacagaccc
    12601 agatagccgt cataaatgcc cattacttcc agaccttctg tcagcgcaga acgaacaacc
    12661 ccgcgaattg cggcgttcat gcctggcgca tcaccgccgc ttgtcaacac accgattttc
    12721 ttaatcatga ctacctctga actttggaat gcaaaataaa atctgttgcc ggaagtcttc
    12781 ttgcacatcg aagtgatcca acgaatgtgc aaatagtata acaatcactt cctgctgaat
    12841 tgattcaggt caggccaaat ggcggtattt tatacacaaa atgcgggtct ggctctcttt
    12901 tatactgatt atgaaagcat agaccgttta ccctccctag gtacgacgga acaggggtcc
    12961 tgatggataa tcacatccga tcccggaaaa cgccgtaaaa tagcctgctc tacctgatcc
    13021 gccaccatat gtgcctgaac caaaggcaga gagtcttcca tttccaaatg aatctgaata
    13081 aagcgggtcg gccctgactg ccgcgtgcga agatcgtgag cgccgctaac acccggccag
    13141 gaagtcacga tatcaataat ttcttgccgt tcctcatcag gtaatgcgcg atccagtaat
    13201 gactgtaccg cctcatatcc catgcgtaac gcgctatata aaatatagat gccgattccc
    13261 aatgcaaaca gagcatcggc gcgatgccag ccgtaccagg acaaccccag cgccagcaga
    13321 attgcgccgt tcatcataac atcagactgg taatgtagca tatcagcccg caccgcctgg
    13381 ctttgcgtcc ggcgcaccac ccaacgctga aacgagacaa ggataatcgt acaaattagc
    13441 gccacaattg tcacgataac cccgacgcct ggatctgtca tcggtgttgg agatatcaga
    13501 tgttgaatgc ctgtcaaaaa caggaatagt gccgaaccgg agataaacat actttgcgcc
    13561 agcgccgcga gggactcagc tttaccgtga ccaaacgagt gattatcgtc ggcaggttgc
    13621 agggaatatc gcaccaccaa taaattcgtc aacgacgcgc cgatatccac cagcgaatcc
    13681 accagcgcgg cgagaatact caccgacccg gtataccacc atgcaaaaat tttaatcagc
    13741 aatagcagtg aagccatcgc cgtcgcagca atcgccgccc gactgaccag ccgtccataa
    13801 gattgattca taaatactcc cgctatcaac tgacgctagt ataacggaag caaatcatct
    13861 gcaatgcatt aagcagcagg caaattgagg ataaaaaaaa cccccacatc atgtggggga
    13921 agacagggat ggtgtctatg gcaaggaaaa cagggtttac tactgggaac gtgagttgct
    13981 actactcaat agcttcaacg atgaactttt ttgccattgc gtcacgtcac gcaactgctc
    14041 cattcgttgt tgatgtttct cgtttaaaac cgcttgctgc tccggcgtta acaggcgata
    14101 catttggttg cggacttttg ccatctcaac ctgacgagca atttgctcat tcgccatttt
    14161 ttctgcctgt gcgcgcacag cgttttcatc aaaattttct gcggtgacaa gacgatgcat
    14221 tgtctccagt tcgctaacat taacaggagg ctgttcgtgc cgggcctgtt gcataagatc
    14281 tcgcatctgc tgacgctgat gttcggttaa acttatgccg tcgaacatat ggctctgcgt
    14341 actgcgctgc gtaagttctt cacccggatg ccagttatcg cctgaaccga cttcagcagc
    14401 gtggcttaat gaactgactg ccagcgttga ggccatgacg gcagcggtaa ctatgcgcat
    14461 catttgctcc caaaatcttt ctgtcgcgat tcaacgatag agagtttacg attcaggctg
    14521 caaacatgcg tcagggggtg taaaacaacg taaagtcatg gattagcgac gtctgatgac
    14581 gtaatttctg cctcggaggt atttaaacaa tgaataaaat cctgttagtt gatgatgacc
    14641 gagagctgac ttccctatta aaggagctgc tcgagatgga aggcttcaac gtgattgttg
    14701 cccacgatgg ggaacaggcg cttgatcttc tggacgacag cattgattta cttttgcttg
    14761 acgtaatgat gccgaagaaa aatggtatcg acacattaaa agcacttcgc cagacacacc
    14821 agacgcctgt cattatgttg acggcgcgcg gcagtgaact tgatcgcgtt ctcggccttg
    14881 agctgggcgc agatgactat ctcccgaaac cgtttaatga tcgtgagctg gtggcacgta
    14941 ttcgcgcgat cctgcgccgt tcgcactgga gcgagcaaca gcaaaacaac gacaacggtt
    15001 caccgacact ggaagttgat gccttagtac tgaatccagg ccgtcaggaa gccagcttcg
    15061 acgggcaaac gctggagtta accggtactg agtttaccct gctctatttg ctggcacagc
    15121 atctgggtca ggtggtttcc cgtgaacatt taagccagga agtgctgggc aaacgcctga
    15181 cgcctttcga ccgcgctatc gatatgcaca tttccaacct gcgtcgtaaa ctgccggatc
    15241 gtaaagatgg tcacccgtgg tttaaaacct tgcgtggtcg cggctacctg atggtttctg
    15301 cttcatgata ggcagcttaa ccgcgcgcat cttcgccatc ttctggctga cgctggcgct
    15361 ggtgttgatg ttggttttga tgttacccaa gctcgattca cgccagatga ccgagcttct
    15421 ggatagcgaa cagcgtcagg gtctgatgat tgagcagcat gttgaagcgg agctggcgaa
    15481 cgatccgccc aacgatttaa tgtggtggcg gcgtctgttc cgggcgattg ataagtgggc
    15541 accgccagga cagcgtttgt tattggtgac caccgaaggc cgcgtgatcg gcgctgaacg
    15601 cagcgaaatg cagatcattc gtaactttat tggtcaggcc gataacgccg atcatccgca
    15661 gaagaaaaag tatggccgcg tggaactggt cggtccgttc tccgtgcgtg atggcgaaga
    15721 taattaccaa ctttatctga ttcgtccggc cagcagttct caatccgatt tcattaactt
    15781 actgtttgac cgcccgctat tactgctgat tgtcaccatg ttggtcagta cgccgctgct
    15841 gttgtggttg gcctggagtc tggcaaaacc ggcgcgtaag ctgaaaaacg ctgccgatga
    15901 agttgcccag ggaaacttac gccagcaccc ggaactggaa gcggggccac aggaattcct
    15961 tgccgcaggt gccagtttta accagatggt caccgcgctg gagcgcatga tgacctctca
    16021 gcagcgtctg ctttctgata tctctcacga gctgcgcacc ccgctgacgc gtctgcaact
    16081 gggtacggcg ttactgcgcc gtcgtagtgg tgaaagcaag gaactggagc gtattgaaac
    16141 cgaagcgcaa cgtctggaca gcatgattaa cgacctgttg gtgatgtcac gtaatcagca
    16201 aaaaaacgcg ctggttagcg agaccatcaa agccaatcag ttgtggagtg aagtgctgga
    16261 taacgcggcg ttcgaagccg agcaaatggg caagtcgttg acagttaact tcccgcctgg
    16321 gccgtggccg ctgtacggca acccgaacgc cctggagagt gcgctggaaa acattgttcg
    16381 taatgccctg cgttattccc atacgaagat tgaagtgggc tttgcggtag ataaagacgg
    16441 tatcaccatt acggtggacg acgatggtcc tggcgttagc ccggaagatc gcgaacagat
    16501 tttccgtccg ttctatcgga ccgatgaagc gcgcgatcgt gaatctggcg gtacaggttt
    16561 gggactggcg attgttgaaa ccgccattca gcagcatcgt ggctgggtga aagcagaaga
    16621 cagcccgctg ggcggtttac ggctggtgat ttggttgccg ctgtataagc ggagttaaac
    16681 tccgcatttg taggcaggat aaggcgttta cgccgcatcc ggcatttgag caggatgcct
    16741 gatgcgacgc tgatagcgtc ttatcaggcc tacactccgg gggttatttt ccccacaacc
    16801 ggcgggaaaa atcttcgatc ttgccgctca gtcggcgctt ttgcatcgtt cgcgtccagc
    16861 ttttcgataa cccggcagcg gagagtaaac ggtgatactg atcgtcatca aacggcatat
    16921 gccatgcgat ggcagcagcc tcctgcacgg tcacatcgct gacacgggaa accaactcca
    16981 gcggcgcgtc cgcagacact ttccctggcg ctatcacgct atacaaccat cccactttac
    17041 ccgtgttttg catcaactgc gctatatcgc tgatatcaaa atggtaattg agtttatagc
    17101 aaggcgagcg cggctggctg acctgaatta atgcctctcc ccagcggaaa atatcgccaa
    17161 tataaacatt actttccgtg agaccgtcgg ttgagagatt ttcgccaaac gcaggcgcaa
    17221 caaacaactc cgcctgttcc ggaaattccc gcgcccagta gagataatgc tcacgaggat
    17281 aatgacacag cgctctgtct ggtccgccgt gaaccttttt ctccgcctgc tcgtcacctt
    17341 ccagccccag ctccgttagc atcaactcac catcaacctg gattttggca attgcgctgg
    17401 gtttgccttc gggataagcc tgaatcttgc ctgtgtatac atcaaccgga tatcgcatct
    17461 gttccctctc cacgagaaat ttccccatta gaccatattt attttatgtc aggcgtgctg
    17521 ttttcgcggt aagtatccag gcaggaactc aatagcaaac cggacgaacg ttagttcatc
    17581 cggctgtggg gattaaggaa gcaggtttat ttcaccgtac ctaaaatttc gattttcgcc
    17641 gctctggctt tgactttacg tgaccagata gaggtaagaa ttggcaccag aatcgaagtc
    17701 acaatgactg ccgtcgctac cagcgaagtt gctgccggag ccatcggttt aaacgcaggc
    17761 accatttctg caatcagcac aggtgtcgct accgcggccc ctgcggaact ggaagcggca
    17821 attccggcag tgccatcgcc accgccaatc aatttatcgg cgataatcaa cggaataccg
    17881 gtcacgataa ttactgccac acccaacagg atccccagca aacctgtctg ggcaattaca
    17941 gtcaaatcaa tggtattgcc tagtgcgaag gcaaagaatg gaatcagcgt ctgcaccgct
    18001 ttgctgaaaa attctcgtaa ttcagggtca aggttcccaa gggcaaagcc caccaggaac
    18061 ggtaatactg cgccgacgaa aacatgcggt tcaaacgagg caatcccggc agtgcccaga
    18121 ataatcatcg tcatgagcgg cccggactcc aacgacatca acacaaatgc cccagcttct
    18181 tcttttgtgc cgtactgctg catgatggaa gcgtaaagtc cgccgttggt catatccatc
    18241 gccgccacca gcgccagcgt tgaaagtccg gcaaagaatc caacttcaac accatgttcc
    18301 ggaataatgc gcgaggcaat tgccgcaacc acccacgcga cggcaatttt agttaccacc
    18361 agcgtaccgg atttacgcag taccgttccc gtcgcgctta attttattga cgcccccatg
    18421 caaaaaaacc acaccgccag aatgggcacc gtaccggtaa tcatcccgtt ggtgaatgat
    18481 ccaaaatatt tccccgcccc cggcgagaag gtgtggcaca gtgcgccaag gaatagcggg
    18541 acgagcatca tcccccccgg gattttctca atcaagcgtt ttatctgcat ttccatcacc
    18601 tgtcggcata tttttaaggg ctgatgcgta attcctcaat ggagcatcag tgttgatgga
    18661 aacgaagata gacattcatt taatcaataa aagtgatcaa aaccacatca actgaaacgc
    18721 tgttttattt ttatcggatc attgttttta ttttgaaatt gatcacaaaa aaaccaccgc
    18781 cattggcgat ggttcatttt ttaagctgat atgcggcgta aacgcctcac tgcagcagac
    18841 ggcaaatgat tattttttcg ccgcgaaacg tgccgctgct tcgtcccagt tcaccacgtt
    18901 ccagaactct ttgatgtagt ccgggcggcg gttctggaat ttcaggtagt aagcatgctc
    18961 ccacacatcc aggcccagaa tcgggaagcc ggatgcgcca gaaatagctt cacccatcag
    19021 cggggagtcc tggttagcag tagaaaccac cgccagttta tcgcctttca gcaccagcca
    19081 tgcccagccg gaaccaaagc gggaagctgc cgctttctca aattctgctt tgaagttatc
    19141 tacggaaccg aaatcacgtt cgatagccgc tttcaggtca ccctgcaggg tggtgccttt
    19201 tttcagacct ttccagaaca ggctgtggtt agcgtgaccg ccagcgttgt tgcgcagtac
    19261 ggttttcttg tctgctggca gctggtccag tttggtgatc aactcttcaa ccggcaggtt
    19321 ggcaaattct ggcaggcttt ccaacgccgc gttggcgttg ttgacgtagg tctgatggtg
    19381 tttggtgtgg tggatttcca tggtctgctt atcgaagtgc ggttccaggg catcgtaagc
    19441 atacggcagg gatggcaggg tatagctcat attcatctcc agtattgtcg ggcggccgat
    19501 tgttaatgcc gcgtaagcag ttggttcatt atagttaatt aaatgatatt gaaaatgatt
    19561 atcaatgccg tacttttcgt aagggtatgg ttttgcagga aaatgcccga gatgtgaagc
    19621 aaatcaccca cttaatgccg tgattgccag taaatcgaca acggcggcaa caggcgaaag
    19681 gttaatcgac agcacgattt ttacactcat ctcgtcggag atgtgacgcg acgaaaaatg
    19741 atgaggataa gaagatgagt aacgcgatta cgatggggat attttggcat ttgatcggcg
    19801 cggccagtgc agcctgtttt tacgctccgt tcaaaaaagt aaaaaaatgg tcatgggaaa
    19861 ccatgtggtc agtcggtggg attgtttcgt ggattattct gccgtaggcc atcagcgccc
    19921 tgttactacc gaatttctgg gcgtattaca gctcgtttag tctctctacg ctgctgcctg
    19981 tttttctgtt cggcgctatg tgggggatcg gtaatatcaa ctacggcctg accatgcgtt
    20041 atctcggcat gtcgatggga attggcatcg ccattggcat tacgttgatt gtcggtacgc
    20101 tgatgacgcc aattatcaac ggcaatttcg atgtgttgat taacaccgaa ggcggacgca
    20161 tgacgttgct cggcgttctg gtggcgctga ttggcgtagg gattgtaact cgagccgggc
    20221 agttgaaaga gcgcaagatg ggcattaaag ccgaagagtt caatctgaaa aaaggactgg
    20281 tgctggcggt gatgtgcggc attttctctg ccgggatgtc ctttgcgatg aacgccgcaa
    20341 aaccgatgca tgaagccgct gccgcactag gcgtcgatcc actgtatgtc gctctgccaa
    20401 gctatgttgt catcatgggc ggcggcgcga tcatcaacct cggtttctgt tttattcgtc
    20461 tggcaaaagt gaaggattta tcgctaaaag ccgacttctc gctggcaaaa ccgctgatca
    20521 ttcacaatgt gttactctca gcactgggcg ggttgatgtg gtatctgcaa ttctttttct
    20581 atgcctgggg ccacgcccgc attccggcgc agtatgacta catcagttgg atgctgcata
    20641 tgagtttcta tgtattgtgc ggcggtatcg tcgggctggt gctgaaagag tggaacaatg
    20701 caggacgccg tccggtaacg gtgttgagcc tcggttgtgt ggtgattatt gttgccgcca
    20761 acatcgttgg catgggcatg gcgaattaat ctttctgcga attgagatga cgccactggc
    20821 tgggcgtcat cccggtttcc cgggtaaaca ccaccgaaaa atagttacta tcttcaaagc
    20881 cacattcggt cgaaatatca ctgattaaca ggcggctatg ctggagaaga tattgcgcat
    20941 gacacactct gacctgccgc agatattgat tgatggtcat tccagtctgc tggcgaaatt
    21001 gctgacgcaa aacgcgctca ctgcacgatg cctcatcaca aaatttatcc agcgcaaagg
    21061 gacttttcag gctagccgcc aaccgggtaa tcagcttatc cagcaacgtt tcgctggatg
    21121 ttggcggcaa cgaatcactg gtgtaacgat ggcgattcag caacatcacc aactgcccga
    21181 acagcaactc tgccatttcg ttagcaaacg gcacatgctg actactttca tgctcaagct
    21241 gaccgataac ctgccgcgcc tgtgccatcc ccatgctacc taagcgccag tgtggttgcc
    21301 ctgcgctggc gctaaatccc ggaatcgccc cctgccagtc aagattcagc ttcagccgct
    21361 ccgggcaata aataatattc tgcaaaacca gatcgttaac ggaagcgtag gagtgtttat
    21421 cgtcagcatg aatgtaaaag agatcgccac gggtaatgcg ataagggcga tcgttgagta
    21481 catgcagacc attaccgcgc cagacaatca ccagctcaca aaaatcatgt gtatgttcag
    21541 caaagacatc ttgcggataa cggtcagcca cagcgactgc ctgctggtcg ctggcaaaaa
    21601 aatcatcttt gagaagtttt aactgatgcg ccaccgtggc tacctcggcc agagaacgaa
    21661 attgattatt cgcaatatgg cgtacaaata cgttgagaag attcgcgtta ttgcagaaag
    21721 ccatcccgtc cctgacgaat atcacgcggt gaccagttaa actctcggcg aaaaagcgtc
    21781 gaaaagtggt tactgtcgct gaatccgcaa tgataggcga tgtcagtaac gctggcctcg
    21841 ctgtgacgta gcagatgtcg ggctttcatc agtcgcaggc gattcaggta tcgctgaggc
    21901 gtcagtcccg tttgctgctt aagctgccga tgtagcgtac gcagtgaaag agaaaattga
    21961 tccgccacgg catcccaatt cacctcatcg gcaaaatggt cctccagcca ggccagaagc
    22021 aagttgagac gtgatgcgct gttttccagg ttctcctgca aactgctttt acgcagcaag
    22081 agcagtaatt gcataaacaa gatctcgcga ctggcggtcg agggtaaatc attttcccct
    22141 tcctgctgtt ccatctgtgc aaccagctgt cgcacctgct gcaatacgct gtggttaacg
    22201 cgccagtgag acggatactg cccatccagc tcttgtggca gcaactgatt cagtccggcg
    22261 agaaactgaa atcgatccgg cgagcgatac agcacattag tcagacacag attatcggta
    22321 tgttcataca gatgccgatc atggtcgcgc acaaaacaga ccgtgccacc ggtgatggta
    22381 tagggctgcc cattaaacac atgaataccc gtgccatgtt cgacaatcac aatttcatga
    22441 aaatcatgat gatgttcagg aaaatccgcc tgcgggagcc ggggttctat cgccacggac
    22501 gcgttaccag acggaaaaaa atccacacta tgtaatacgg tcatactggc ctcctgatgt
    22561 cgtcaacacg gcgaaatagt aatcacgagg tcaggttctt accttaaatt ttcgacggaa
    22621 aaccacgtaa aaaacgtcga tttttcaaga tacagcgtga attttcagga aatgcggtga
    22681 gcatcacatc accacaattc agcaaattgt gaacatcatc acgttcatct ttccctggtt
    22741 gccaatggcc cattttcctg tcagtaacga gaaggtcgcg aattcaggcg ctttttagac
    22801 tggtcgtaat gaaattcagc aggatcacat tatgaccttt cgcaattgtg tcgccgtcga
    22861 tctcggcgca tccagtgggc gcgtgatgct ggcgcgttac gagcgtgaat gccgcagcct
    22921 gacgctgcgc gaaatccatc gttttaacaa tgggctgcat agtcagaacg gctatgtcac
    22981 ctgggatgtg gatagccttg aaagtgccat tcgccttgga ttaaacaagg tgtgcgagga
    23041 agggattcgt atcgatagca ttgggattga tacctggggc gtggactttg tgctgctcga
    23101 ccaacagggt cagcgtgtgg gcctgcccgt tgcttatcgc gatagccgca ccaatggcct
    23161 aatggcgcag gcacaacaac aactcggcaa acgcgatatt tatcaacgta gcggcatcca
    23221 gtttctgccc ttcaatacgc tttatcagtt gcgtgcgctg acggagcaac aacctgaact
    23281 tattccacac attgctcacg ctctgctgat gccggattac ttcagttatc gcctgaccgg
    23341 caagatgaac tgggaatata ccaacgccac gaccacgcaa ctggtcaata tcaatagcga
    23401 cgactgggac gagtcgctac tggcgtggag cggggccaac aaagcctggt ttggtcgccc
    23461 gacgcatccg ggtaatgtca taggtcactg gatttgcccg cagggtaatg agattccagt
    23521 ggtcgccgtt gccagccatg ataccgccag cgcggttatc gcctcgccgt taaacggttc
    23581 acgtgctgct tatctctctt ctggcacctg gtcattgatg ggcttcgaaa gccagacgcc
    23641 atttaccaat gacacggcac tggcagccaa catcaccaat gaaggcgggg cggaaggtcg
    23701 ctatcgggtg ctgaaaaata ttatgggctt atggctgctt cagcgagtgc ttcaggagcg
    23761 gcaaatcaac gatcttccgg cgcttatctc cgcgacacag gcacttccgg cttgccgctt
    23821 cattatcaat cccaatgacg atcgctttat taatcctgag acgatgtgca gcgaaattca
    23881 ggctgcgtgt cgggaaacgg cgcaaccgat cccggaaagt gatgctgaac tggcgcgctg
    23941 cattttcgac agtctggcgc tgctgtatgc cgatgtgttg catgagctgg cgcagctgcg
    24001 cggtgaagat ttctcgcaac tgcatattgt cggcggaggc tgccagaaca cgctgctcaa
    24061 ccagctatgc gccgatgcct gcggtattcg ggtgatcgcc gggcctgttg aagcctcgac
    24121 gctcggcaat atcggcatcc agttaatgac gctggatgaa ctcaacaatg tggatgattt
    24181 ccgtcaggtc gtcagcacca ccgcgaatct gaccaccttt acccctaatc ctgacagtga
    24241 aattgcccac tatgtggcgc ggattcactc tacacgacag acaaaggagc tttgcgcatg
    24301 accactcaac tggaacaggc ctgggagctg gcgaaacagc gtttcgcggc agtagggatt
    24361 gatgtcgagg aggcgctgcg ccaacttgat cgtttacccg tttcaatgca ctgctggcag
    24421 ggtgatgatg tttccggttt tgaaaacccg gaaggttcgc tgaccggagg gattcaggct
    24481 actggcaatt atccgggcaa agcgcgtaat gccagtgagc tacgtgccga tctggaacag
    24541 gctatgcggc tgattccggg gccaaaacgg cttaatttac atgccatcta tctggaatca
    24601 gacacgccag tctcgcgcga ccagatcaaa cctgagcact tcaaaaactg ggttgaatgg
    24661 gcgaaagcca atcagctcgg tctggatttt aacccctcct gtttctcgca tccgctaagc
    24721 gccgatggct ttacgctttc ccatgccgac gacagcattc gccagttctg gattgatcac
    24781 tgcaaggcca gccgccgcgt ttcggcctat tttggtgagc aactcggcac accgtcggtg
    24841 atgaacatct ggatcccgga tggcatgaaa gatatcaccg ttgaccgtct cgctccgcgc
    24901 cagcgtctgc tggcagctct ggatgaggtg atcagcgaga agctggatcc ggcgcaccat
    24961 atcgacgccg ttgagagcaa attgtttggc attggcgcag agagctacac ggttggctcc
    25021 aatgagtttt acatggggta tgccaccagc cgccagactg cgctgtgcct ggacgccggg
    25081 catttccacc cgactgaagt gatttccgac aagatttccg ccgccatgct gtatgtgccg
    25141 cagttgctgc tgcacgtcag ccgtccggtt cgctgggaca gcgatcacgt agtgctgctg
    25201 gatgatgaaa cccaggcgat tgccagtgag attgttcgtc acgatctgtt tgaccgggtg
    25261 catatcggcc tcgacttctt tgatgcctct atcaaccgta ttgctgcgtg gatcattggt
    25321 acacgcaata tgaaaaaagc cctgctgcgt gcgttgctgg aacctaccgc tgagctgcgc
    25381 aagctggaag cggcgggcga ttacactgcg cgtctggcac tgctggaaga gcagaaatcg
    25441 ttgccgtggc aggcggtctg ggaaatgtat tgccaacgtc acgatacgcc agcaggtagc
    25501 gaatggctgg agagcgtgcg ggcatatgag aaagaaactt tgagtcgccg cgggtaaaca
    25561 ctgccggatg cggcacaagt gccttatccg gcctacaggc cggcaatagt tgtaggcctg
    25621 ataagacgcg acagcgtcgc atcaggcatt gattgccgga tgcggcacga gcgccttatc
    25681 cggcctacgg gtcgtgcatc tgacaacacc gaatttacag gaacacagaa catgcaaaac
    25741 attacccagt cctggtttgt ccagggaatg atcaaagcca ccaccgatgc ctggctgaaa
    25801 ggctgggacg agcgcaacgg cggcaacctg acgctgcgcc tggatgacgc cgatatcgcg
    25861 ctttatcacg acaacttcca cccacaaccg cgctatattc cgctcagcca acccatgcct
    25921 ttactggcaa atacgccgtt tattgtcacc ggctccggca aattcttccg taacgtccag
    25981 ctcgatcctg cagctaactt aggcgtcgta aaagtcgaca gcgatggcgc gggctaccat
    26041 attctctggg ggttaaccaa cgaagccgtt ccaacttctg aacttccggc tcactttctc
    26101 tcccactgcg agcgcattaa agccaccaac ggtaaagatc gggtgatcat gcactgccac
    26161 gccaccaacc tgatcgccct cacctatgta cttgaaaacg acaccgcggt cttcactcgc
    26221 caactgtggg aaggcagcac cgagtgtctg gtggtattcc cggatggcgt tggcattctg
    26281 ccgtggatgg tacccggcac ggacgaaatc ggccaggcaa ctgcccaaga gatgcaaaaa
    26341 cattcgctgg tgttgtggcc cttccacggt gtgttcggtt ccggaccgac gctggatgaa
    26401 accttcggtt taatcgacac cgcagaaaaa tcagcggaag tgttagtgaa ggtgtattcg
    26461 atgggcggca tgaaacagac tatcagccgt gaagagttga tagcgctcgg ccagcgtttc
    26521 ggcgttacac cactcgccag tgcgctggcg ctgtaaggag caaacatgat ccgcaaagcc
    26581 tttgtcatgc aggtaaaccc cgacgcccac gaagagtatc agcgtcggca taatcccatc
    26641 tggccggaac tggaagcagt gttgaaatct cacggtgcgc ataactacgc catttacctc
    26701 gacaaagcgc gtaatctgct gtttgccacg gtagagattg aatctgaaga acggtggaat
    26761 gcggtagcca gcactgaaat ctgccaacgt tggtggaaat atatgaccga tgttatgccc
    26821 gctaacccgg ataacagccc ggtgagtagc gagctgcaag aagtgtttta cctgccttaa
    26881 tgagttgcgg tgccggaaaa tatccagcac cgcgacttca tcagaagaag tatttaaagc
    26941 gaacgcgtgc gccgtaacgg tcatcagtat catcaccgtg ttttttatcg ccgtcgctat
    27001 ccagaatgga gtaatacgtc ccgaggtaaa tattaaagtt ctccatatcc atcacgttag
    27061 cgaactgata agacgcatga atggtgtgaa tggtgtacgt cccttcatca tcaatccagc
    27121 agtcgttatc acaaactaca ccgctgaatt catcaatctt attgtgcgcg tagatataac
    27181 cgagctcgaa acgtttccac agcgcgttaa tcccggcagt gaaatctttc tcattattag
    27241 catctaaata ggcggtattt agattaacca caatgccatt gtccggatcc gttttcaggc
    27301 cgttccaggt catgctcata ccatagccgg tacgatcgga ctgatcgaca aaattaccct
    27361 tgctatcggt ataaccataa gcattattta ccacgttcgc ttccatcgct gcggaaacgg
    27421 tgaattcttc cgtcggcgac caggcaatta ctgggcgcag ataagcaaca tttttctgct
    27481 gctccatatc gcgtccatga taattaccgt cattatataa agatgttccg tcttcaagta
    27541 acgtgtttaa ttcaaaatac cagttatcga gttgtttgct gacgaggaaa ttaccgccag
    27601 cgttagagcg cccgcggccc tctttcatca tatagatata accgctgcca tcgtcataaa
    27661 gatcgttcgc agtattaccg gaatgttcaa caaaggtatc ctgattcaat gggaacatat
    27721 cgtaggcttc aaaacggcct actttgacct tccagtcgtt ttctttgcca aagaagaata
    27781 ccgcatcatc gatatttact gaaccgtgca tatcacccag cggttgtgcg gagaacccgg
    27841 cgaaataacc gttgtccatc tttcgcatac cgtcaaaacc taacagaata cgaccattga
    27901 gattccactg ttcattagtg ggatcgctat tcacatcagc gttggtcatt gccagcaggc
    27961 catgattact ttccgcatcc atattaaatt ctacgtcgcc gtagattttt aaatcgccat
    28021 aacctgaaag caaaagatta ggtgaggtgg catttttctt tgcagattcc gccgttgcct
    28081 gtccatttac cggcgtattg ccctgagcgg ccttaatttc gcggatctca gcggcttgtt
    28141 gtgtctgcag ctgtttaacc tggctctctg cttttgccgc ccgcatttct gccgcttcca
    28201 ggcgttcttc cagcaatgcc atgcgttgtt ccagcattaa atgctgtgtt tttgccatac
    28261 cggtactaca tgccaataat aaaccgatgg ctaaagataa tgtcgatttt ttcattattt
    28321 acgttccctg aaataaagga gaccctacgc tttccaaaag gaatacgtat atagagaaat
    28381 ataaggtgtg cactttcgaa tttttaatcc aacttcagca ggccgatgga tagtatcccg
    28441 ccgcataata tgagtaaatc cgctgatttc ctggaacgtg aggaatataa caatagtgcc
    28501 gtttttaact aaatatcgta aaaaatatta gatatgattc aagctggctc ataagccaca
    28561 aatataaata acgtttagac aaattatctg agacatttaa acattcacaa aatcattaaa
    28621 taataactct ttattcacaa gatttatttt acctactaat atgtgcctta cgccgcaaaa
    28681 tttatccttc actctgcttt atattgtcac gacttcacga catatccgcg ctcatcacaa
    28741 acccgccccg ctattacaat taactgaaat attttttcat ggagtccgat taatggcagc
    28801 tcttactgca agctgtattg acctgaatat tcagggcaat ggcgcttatt ccgttctgaa
    28861 gcagttggcg acaatggcgt tacaaaacgg ttttatcacc gactcacacc agttcctgca
    28921 aaccctgctg ctgcgcgaaa aaatgcactc caccggtttt ggttctggtg tcgccgtgcc
    28981 gcacggtaaa agcacctgcg ttaaacaacc gttcgtgtta ttcgcccgca aagcgcaggc
    29041 tattgactgg aaagccagcg acggcgaaga cgtcaattgc tggatctgcc tcggcgtacc
    29101 gcaaagtggc gaagaggatc aggtcaaaat catcggcaca ctgtgtcgca aaattattca
    29161 ccaggaattt attcatcaac tgcaacaggg cgataccgac caggtgcttg ccttgttaaa
    29221 tcaaaccctc agctcataag gaagtggcga tggagtcatc cttacgtatt gtcgcgatca
    29281 ccaactgccc cgccgggatc gctcacacct acatggtggc ggaagccctg gaacagaaag
    29341 cccgttctct cggccatacc attaaagtgg aaactcaagg gtccagcggc gttgaaaacc
    29401 gcttatccag cgaagagatt gccgctgccg attacgtgat tctcgccacc gggcgtggcc
    29461 tgagcggtga cgatcgcgca cggtttgccg ggaaaaaagt ttatgagatt gccatctccc
    29521 aggcgctgaa aaatatcgat cagattttca gtgaattacc gacaaattcg cagctttttg
    29581 ccgcagatag cggcgtgaag ctaggcaaac aggaagtaca aagcggcagc gtgatgagcc
    29641 acctgatggc gggcgtctct gcggcgctgc cgtttgtcat cggcggtggt atcctggtgg
    29701 cgcttgccaa catgctggtg cagttcggtt tgccgtatac cgatatgtcg aaaggcgcgc
    29761 cgtcgtttac ctgggtagtt gaatccatcg gttaccttgg cttcaccttt atgatcccca
    29821 tcatgggcgc ttatattgcc tcgtcgattg ccgataaacc ggctttcgct ccggcgtttc
    29881 tggtttgcta tctggcgaac gacaaagcgc tgctcggcac ccagtcgggt gcgggattcc
    29941 tcggcgcggt ggtgctgggg ctggcgattg gctatttcgt cttctggttc cgtaaagtcc
    30001 gtcttggcaa agcattacaa ccgctgctcg gctccatgct tatcccattc gttactctgc
    30061 tggtttttgg cgtgctcact tactacgtta tcgggccggt gatgtccgac ctcatgggcg
    30121 ggctactgca cttcctgaat accattcctc catcaatgaa gtttgcggca gcgtttctgg
    30181 tcggtgcgat gctggcgttc gatatgggcg gccccatcaa taaaaccgcc tggttcttct
    30241 gcttctcact gctggaaaaa cacatttatg actggtacgc catcgtcggc gttgtccccg
    30301 tcgcggcggg tcttgcgacg ttcattgcgc ccaaactgtt tacccgacag gaaaaagagg
    30361 ctgccagtag tgccattgtg gtcggtgcca cagtcgcaac cgaaccggct attccttacg
    30421 ctctggcagc tccgttgccg atgattaccg ccaatacgct ggcgggcggc attaccggcg
    30481 tgctggtgat cgctttcgga atcaaacgtc tggcacctgg tttgggtatc tttgacccgc
    30541 tgatcggcct gatgtcgccg gtaggttcgt tttatctggt gctggcgatt ggcctcgcgc
    30601 tgaacatttc attcattatc gtcctgaaag gattgtggtt acgccgcaaa gcgaaagccg
    30661 cgcaacagga gcttgtccat gaacattgag ttactgcaac agttgtgcga agccagcgcc
    30721 gtcagcggcg atgaacagga agttcgcgac attctgataa acacgctgga accttgcgtt
    30781 aatgagatca cctttgatgg tctgggcagc tttgttgccc gtaagggaaa taaaggtcca
    30841 aaagttgccg ttgtcgggca tatggatgaa gtcggcttta tggtcaccca catcgacgag
    30901 agcggttttc tgcgctttac caccattggc ggctggtgga atcagtcgat gctcaaccac
    30961 cgggtaacaa tacgcacaca caagggagtg aaaatccctg gtgtgattgg ttccgtagcg
    31021 cctcatgcgt taacggaaaa gcaaaagcaa caaccgctgt catttgatga gatgttcatt
    31081 gatattggcg cgaacagtcg cgaagaagcg aaaaaacgcg gtgcggaaat tggcgatttt
    31141 attagcccgg aagccaattt tgcctgctgg ggcgaagata aaatagtcgg caaggcgctg
    31201 gataatcgca tcggctgcgc gatgatggct gagctactac agacagtaaa taacccagga
    31261 attacgctgt acggcgtcgg cagcgtggaa gaagaagttg ggctacgcgg ggcacaaacc
    31321 tcggctgaac acactaaacc ggatgtggtg atcgtgctgg ataccgccgt cgcgggcgat
    31381 gttccgggca ttgataacat taaatacccg ctgaaactgg gcaacgggcc ggggctgatg
    31441 ctgtttgaca agcgctactt acccaaccag aaactggtgg cggcgttaaa aaactgtgcc
    31501 gcacataacg gtttaccgct gcaattttcc accatgaaaa ccggagcgac ggatggcggg
    31561 cgctacaacg taatgggcgg agggcgtccg gttgtcgcgc tgtgtctgcc aactcgttat
    31621 ctgcacgcta acagcggtat gatttcaaaa gccgattatg atgctctgct cacgctgata
    31681 cgggattttc tgacgacctt aactgcggag aaagtcaacg cgtttagcca gttccgtcag
    31741 gtggattaat gctaaacgaa cgccagttaa agattgtcga tctgctggag caacagccgc
    31801 gcacgcctgg cgagctggcg caacagactg gcgtttcagg caggaccatc ctgcgtgata
    31861 ttgactatct caactttacc cttaacggca aagctcgtat ctctgccagt ggcagtgcgg
    31921 gctatcagtt ggaaatcttc gagcgccgca gcttttttca gttgctgcaa aagcacgata
    31981 acgacgatcg gctgctggcg ctgttattac tgaatacctt tactccccgt gcgcaactcg
    32041 cctcggcgct taatttgcca gaaacttggg tcgcagagcg tctgccccgg ttaaaacagc
    32101 gttatgaacg cacttgttgc ctggccagtc gtcccggttt gggccatttc attgatgaaa
    32161 cagaagagaa acgcgttatc ttgctggcga acttgctacg caaagatccg tttttaatac
    32221 ctttggctgg cgtaacacga gacaaccttc agcatttatc cacggcctgc gacaaccaac
    32281 accgctggcc gctcatgcag ggggattatc tctccagcct gattctggcg atttacgccc
    32341 tgcgtaatca actgaccgat gagtggccgc aatatcccgg tgacgagata aaacaaatcg
    32401 ttgaacataa cggtctgttt cttggtgata acgctgtaag aaccctgaca ggtttgatag
    32461 agaaacagca tcagcaagcg caggtaattt ctgccgatca tgtgctgggg ttgctgcaaa
    32521 gggtgccagg tctcgcgtca ttgaatatta ttgatacgca gctggttgag aacattaccg
    32581 gtcatttatt acgttgcctt gccgcaccag tgtggattgc tgagcatcgc caaagcagca
    32641 tgaataacct gaaagccgcc tggcctgcgg cctttgatat gagtctgcac tttattacgc
    32701 tactgcgtga acagctcgat attccccttt tcgacagcga tctgatcggt ttgtattttg
    32761 cctgtgcgct ggaacgacat caaaacgaac gccagccgat cattttgctc tcggaccaga
    32821 acgcgattgc cactattaat cagctcgcca ttgagcgcga tgttttacat tgtcgggtga
    32881 ttattgcccg tagcttaagc gaacttgttg ccattcgcga agagattgag ccgttattga
    32941 tcattaacaa cagccattat ttactggatg acgcggtaaa taattacatc accgtaaaaa
    33001 atatcattac ggctgccggt atcgaacaaa taaaacattt tctggcgacg gcatttattc
    33061 gccagcaacc ggagcgtttt ttctctgccc ccggaagttt tcattattcg aatgtacgcg
    33121 gtgaaagctg gcaacatatt acccggcaaa tttgtgcgca attagtcgca caacaccata
    33181 ttaccgccga tgaagcgcaa cggatcatcg cccgcgaagg cgaaggtgaa aacctgattg
    33241 ttaatcgcct cgccatccca cattgctgga gcgaacagga gcgacgtttt cgtggatttt
    33301 ttattaccct cgcccaacca gttgaggtga ataacgaagt cattaaccat gtcttgatcg
    33361 cctgcgccgc cgccgatgcg cgtcatgagc taaaaatatt tagctatctg gcaagcgtat
    33421 tgtgtcagca tccggcagaa gttattgccg ggctaacagg atatgaggca tttatggagt
    33481 tacttcacaa ggggtgaact gtgtcggatg cgccgcatcc gacactgcat ttatcatcaa
    33541 aaacgtttaa cgcaggctat tatagtcatc aaccatttcg ttgtactcgc gtaacgctcg
    33601 tggaaatgag tcttcgacca tccagcttgt gtttgcatcc tgaagatgct ctttatccca
    33661 gtcagagtac gggactttat ctctgacgcg gcgaacatat tttttagtct cgagttgata
    33721 ctggtctgcc gaattaatga acgagaaatt catgccgcct ttatccgctt ctttggcctg
    33781 cgccaccagc gtttccagtt cagacacttt cttcattgcc gcatcaacat caaacttctc
    33841 ttgcgatatc aggttattaa tttgtttcgc tgaaatcatg actgccagag aatagtagtg
    33901 gaatgttttc ccttctcttt cttcaatatt tttcagttcc gcaagctggc gcttatcatt
    33961 aatttcctga atcgccgcat gataagattc tgctacaggt tcaaaggctt ccagattttt
    34021 taagaatgtc tggtgcagcg ttttcccttt cgcaaacgca tcgtctttat aattttcctg
    34081 ggtgtaatat ttatccattt cgttaatggt attacccaac gccacggcag catcgatata
    34141 actcaccgcc acgccatcaa ttggttgcag cgcaggcgtt aacgccacag cactttttac
    34201 gcctttttca cactcagcga ggttggattc actaatgccg tagatgccat aaacagtact
    34261 ctctttaccg gtaggcccct gtttaaaatc tttcagccag tcagcataac gcgccagact
    34321 gcgctgtacc gggatctgta acttgttgta gcacttgata tagacatcca ttttttcgtc
    34381 gaaaaccgct tgttcatcga ccagcggttg cgcaggtgtt tcaggtttag catctgcttt
    34441 tgcaacggga gcttcagtag gtttcgcttc aggagctggt gcggcaggtt gactattggc
    34501 aggcaggggc gtttctgttt cggctttttt atcatcacaa cccgtcagac cgagggccat
    34561 gatggcgact ataatagcgg aagataataa attcctttt
//
LOCUS       DAZFAB010000038        34300 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZFAB010000038 DAZFAB010000000
VERSION     DAZFAB010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.038"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(36..926)
                     /gene="cyoE"
                     /locus_tag="ACHJWA_002203"
     CDS             complement(36..926)
                     /gene="cyoE"
                     /locus_tag="ACHJWA_002203"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFO4308799.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(938..1267)
                     /gene="cyoD"
                     /locus_tag="ACHJWA_002204"
     CDS             complement(938..1267)
                     /gene="cyoD"
                     /locus_tag="ACHJWA_002204"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFO4308800.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(1267..1881)
                     /gene="cyoC"
                     /locus_tag="ACHJWA_002205"
     CDS             complement(1267..1881)
                     /gene="cyoC"
                     /locus_tag="ACHJWA_002205"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFO4308801.1"
                     /translation="MATDTLTHATVHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(1871..3862)
                     /gene="cyoB"
                     /locus_tag="ACHJWA_002206"
     CDS             complement(1871..3862)
                     /gene="cyoB"
                     /locus_tag="ACHJWA_002206"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFO4308802.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(3884..4831)
                     /gene="cyoA"
                     /locus_tag="ACHJWA_002207"
     CDS             complement(3884..4831)
                     /gene="cyoA"
                     /locus_tag="ACHJWA_002207"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFO4308803.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGFATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAAFDQWVAKAKQSPNSMSDMAAFEKLAAPSEYNQVEYFSNVKPNLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            complement(5291..6766)
                     /gene="ampG"
                     /locus_tag="ACHJWA_002208"
     CDS             complement(5291..6766)
                     /gene="ampG"
                     /locus_tag="ACHJWA_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFO4308804.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTAYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(6810..7388)
                     /gene="yajG"
                     /locus_tag="ACHJWA_002209"
     CDS             complement(6810..7388)
                     /gene="yajG"
                     /locus_tag="ACHJWA_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO4308805.1"
                     /translation="MFKKILFPLVALFMLAGCARPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            7693..8010
                     /gene="bolA"
                     /locus_tag="ACHJWA_002210"
     CDS             7693..8010
                     /gene="bolA"
                     /locus_tag="ACHJWA_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFO4308806.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            8354..9652
                     /gene="tig"
                     /locus_tag="ACHJWA_002211"
     CDS             8354..9652
                     /gene="tig"
                     /locus_tag="ACHJWA_002211"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFO4308807.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            9897..10520
                     /gene="clpP"
                     /locus_tag="ACHJWA_002212"
     CDS             9897..10520
                     /gene="clpP"
                     /locus_tag="ACHJWA_002212"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFO4308808.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            10646..11920
                     /gene="clpX"
                     /locus_tag="ACHJWA_002213"
     CDS             10646..11920
                     /gene="clpX"
                     /locus_tag="ACHJWA_002213"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFO4308809.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            12108..14462
                     /gene="lon"
                     /locus_tag="ACHJWA_002214"
     CDS             12108..14462
                     /gene="lon"
                     /locus_tag="ACHJWA_002214"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFO4308810.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILNTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            14653..14943
                     /gene="hupB"
                     /locus_tag="ACHJWA_002215"
     CDS             14653..14943
                     /gene="hupB"
                     /locus_tag="ACHJWA_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFO4308811.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            15135..17006
                     /gene="ppiD"
                     /locus_tag="ACHJWA_002216"
     CDS             15135..17006
                     /gene="ppiD"
                     /locus_tag="ACHJWA_002216"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO4308812.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADLQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDVAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKENLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            17157..17528
                     /gene="ybaV"
                     /locus_tag="ACHJWA_002217"
     CDS             17157..17528
                     /gene="ybaV"
                     /locus_tag="ACHJWA_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFO4308813.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QNKAAVPAKASDEEGSRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            17634..18032
                     /gene="fadM"
                     /locus_tag="ACHJWA_002218"
     CDS             17634..18032
                     /gene="fadM"
                     /locus_tag="ACHJWA_002218"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFO4308814.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFHWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(18084..18779)
                     /gene="queC"
                     /locus_tag="ACHJWA_002219"
     CDS             complement(18084..18779)
                     /gene="queC"
                     /locus_tag="ACHJWA_002219"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFO4308815.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKVLNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLK"
     gene            complement(18844..20544)
                     /gene="ybaE"
                     /locus_tag="ACHJWA_002220"
     CDS             complement(18844..20544)
                     /gene="ybaE"
                     /locus_tag="ACHJWA_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFO4308816.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEKALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSADGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMTDATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            20644..21462
                     /gene="cof"
                     /locus_tag="ACHJWA_002221"
     CDS             20644..21462
                     /gene="cof"
                     /locus_tag="ACHJWA_002221"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFO4308817.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGAISLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLHEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            21614..22072
                     /gene="decR"
                     /locus_tag="ACHJWA_002222"
     CDS             21614..22072
                     /gene="decR"
                     /locus_tag="ACHJWA_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFO4308818.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            22102..23874
                     /gene="mdlA"
                     /locus_tag="ACHJWA_002223"
     CDS             22102..23874
                     /gene="mdlA"
                     /locus_tag="ACHJWA_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO4308819.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLAQQSGWYRD
                     MYRYQQLEAALDDAPENREEAVDA"
     gene            23867..25648
                     /gene="mdlB"
                     /locus_tag="ACHJWA_002224"
     CDS             23867..25648
                     /gene="mdlB"
                     /locus_tag="ACHJWA_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO4308820.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            25829..26167
                     /gene="glnK"
                     /locus_tag="ACHJWA_002225"
     CDS             25829..26167
                     /gene="glnK"
                     /locus_tag="ACHJWA_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFO4308821.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            26197..27483
                     /gene="amtB"
                     /locus_tag="ACHJWA_002226"
     CDS             26197..27483
                     /gene="amtB"
                     /locus_tag="ACHJWA_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFO4308822.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(27532..28392)
                     /gene="tesB"
                     /locus_tag="ACHJWA_002227"
     CDS             complement(27532..28392)
                     /gene="tesB"
                     /locus_tag="ACHJWA_002227"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFO4308823.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            28610..29182
                     /gene="ybaY"
                     /locus_tag="ACHJWA_002228"
     CDS             28610..29182
                     /gene="ybaY"
                     /locus_tag="ACHJWA_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFO4308824.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(29215..29526)
                     /locus_tag="ACHJWA_002229"
     CDS             complement(29215..29526)
                     /locus_tag="ACHJWA_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFO4308825.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            29689..29785
                     /gene="ffs"
                     /locus_tag="ACHJWA_002230"
     ncRNA           29689..29785
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHJWA_002230"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            29905..30258
                     /gene="ybaA"
                     /locus_tag="ACHJWA_002231"
     CDS             29905..30258
                     /gene="ybaA"
                     /locus_tag="ACHJWA_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFO4308826.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(30300..31850)
                     /gene="pdeB"
                     /locus_tag="ACHJWA_002232"
     CDS             complement(30300..31850)
                     /gene="pdeB"
                     /locus_tag="ACHJWA_002232"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFO4308827.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGADCSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASSKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(32014..32484)
                     /gene="ylaC"
                     /locus_tag="ACHJWA_002233"
     CDS             complement(32014..32484)
                     /gene="ylaC"
                     /locus_tag="ACHJWA_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFO4308828.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(32600..33151)
                     /gene="maa"
                     /locus_tag="ACHJWA_002234"
     CDS             complement(32600..33151)
                     /gene="maa"
                     /locus_tag="ACHJWA_002234"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFO4308829.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     ALGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(33323..33541)
                     /gene="hha"
                     /locus_tag="ACHJWA_002235"
     CDS             complement(33323..33541)
                     /gene="hha"
                     /locus_tag="ACHJWA_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFO4308830.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(33567..33941)
                     /gene="tomB"
                     /locus_tag="ACHJWA_002236"
     CDS             complement(33567..33941)
                     /gene="tomB"
                     /locus_tag="ACHJWA_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFO4308831.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
BASE COUNT         8750 a         8326 c         9154 g         8070 t
ORIGIN      
        1 aggtgccgta gcaccttttt aatagagagg ttgtgttacc acacagcagc cagcagcgta
       61 tgcgagtccg gcaccataaa atcaacggac atcatcaccg agagggcagt gatggcgatg
      121 atagagaagc cgaacagctt gcgcgcccag attctgtcat cagcaacttt ataaccgcgc
      181 agagccatac ctaaccacca gacgctaacc gccgcggcga ccaccagata tttatatcca
      241 gcgtaaccgc caagagagag catcagcgtg gcaacggcaa aggcgatgat atacagcgtg
      301 atgtgattct tcgccaccga aatgcctttt accactggca ataccggaat gtttgccgcc
      361 tggtaatcct taaagcggaa aatggcgatg gcataggagt gaggcatctg ccacaggctg
      421 aagatagcca gcaggatcgc tgcgccgcta tcgaactcgc cggttactgc acagtagcca
      481 atcaccggcg gcgcagcgcc ggagagcgaa ccaatcaatg tgccgtagac ggagtggcgt
      541 ttcatgtaca ggctataaac gccgacataa accacaaagc ccatcacccc cagccagcag
      601 gccagcggat tcgcgccaaa ccacagcagc ataaagccag caatacccag caaggtggcg
      661 tacaccagcg agacagcagg agagatcagg cctttaacca gcacccgatt cttcgtcctt
      721 tccatctttc tgtcgatatc cctgtcgatg tagttgttaa acacacaacc cgacgccaca
      781 accagtgaca ccccaaccag cgtgtagata aacaggggat aatcaatgct gccctttgag
      841 gccagcagga atcccccaat caccgagatc aggttgccaa agatgatgcc tggtttcgtt
      901 acttgcaggt attgcttaaa catcataacc gccgctctta gtgcatcatc atgttgtagt
      961 tgaggttcca cataatccag atggagccta caactaggat agcgatgatt agcacggtga
     1021 agacaaacgc cgtcatgttc cagccttcat ctgatttggt attcatgtgc aggaagcaca
     1081 ccagatgcac cagaacctgt accactgcca ttgccaggat tgttcccaga attacggccg
     1141 gagaggcagc ccctgtcatc accatccaga acggaatcac cgtcagaatg atcgacagga
     1201 taaagcctgt catgtaggtt tttacgctgc catgggacgc gccgctgtga tcggtagaat
     1261 gactcattac atcgccccca tcagataaac aacagtgaac acacagatcc aaaccacatc
     1321 caggaagtgc cagaacaggc tcaggcacat gatgcgggta cggttagtgc tggtcaggcc
     1381 gcgacgagcg atttgtacca tcagcaccgc catccagata agaccggaag tgacgtgcag
     1441 accgtgcgtg ccgaccagcg caaagaacgc tgacaggaag ccgctgcgat ccggacccat
     1501 gccgttaaca atcaggtgat ggaattcgta gatttccatc ccgataaatc cggcgccaaa
     1561 caaccaggtc aacgccagcc aggagataac ctggcttttg ttgtttttgt acatggcgat
     1621 agccgccatg ccgtaggtga tggagctgaa caacagcaag aaagtttcaa ccagaacgaa
     1681 cggcagttcg aaaatgtcct tacctgtcgg gccgcctgcg gtgccgttca ccagaacggc
     1741 ataggtagca aacaagatag agaacagaat gcagtcgctc atcaggtaga tccaaaatcc
     1801 gaagattttg gttccgcctg catcgtggtg cccgtgttcg tgcgcgtgga cagtcgcgtg
     1861 cgtcaaagta tcagttgcca tttttcagcc ctgccttagt aatctcatcg aaatgctggt
     1921 tttccagttt ttcgatttct gccaccggca cgtagtaatc cacgtcctcg tcgaagcttt
     1981 tcacgatcca ggtgatgatc atgcctgcga agccaacaat cgccagccac cagatatgcc
     2041 agatcatggc gaaaccgaag atggtggaga aagccgcaat gacgatacct gcaccgctgt
     2101 ttttcggcat atgaatttct tcatagtggt caggcttttt atacgcttcg cctttctctt
     2161 tcatttccca gaatgcatca cgctcgtgaa cgtgcggcac aacggcaaag ttatagaacg
     2221 gaggcgggga agaggttgcc cactccagcg tacggccacc ccacgggtcg ccagtcaggt
     2281 cacggttctg gtcgcggtca cgaatagaaa cgtacatctg aataacgagg cagagaatac
     2341 ctagcgcaat cagtactgca ccgctggctg caatcatcag catggtgtgg aactgcgggt
     2401 caatctgctg gctcaaacga cgggtcatgc ccatgaagcc cagcgcgtac agtggcataa
     2461 aggcaacgaa gaagccgatg atccagaacc aaaacgcgcg tttaccccag gtttcgttca
     2521 gtttgaaacc gaacgcttta ggccaccagt aggtcatccc tgcgaagcag ccgaagacca
     2581 cgccgccgat gatcacgtta tggaagtgcg caatcaggaa caggctgtta tgtagaacga
     2641 agtccgcgcc cggtactgcc agcagcacgc cagtcatacc gcccaccgag aaggtgacga
     2701 taaaaccgat agtccacagc atcgcagaat ggaacacgat gcggccctga tacatggtga
     2761 acagccagtt gaagatcttc accccggtcg ggatggcgat aatcattgtg gtgataccaa
     2821 agaaggcgtt tacgttcgcg cccgcaccca tcgtaaagaa gtggtgcagc caaacgatga
     2881 acgacagcac ggtgatacag acggttgccc ataccagcga ggtataacca aacagacgtt
     2941 tacgcgagaa ggttgccgca atttcggaga acacaccgaa aacaggcagg atcaggatgt
     3001 aaacttccgg gtggccccag gcccaaatca ggttgatgta catcatcatg ttgccaccca
     3061 tatcgttggt aaagaaatgg gtgcccagat agcgatccag ggtcaacaac gcgacggtaa
     3121 ccgtcagaat tgggaaggaa gcaataatca gtacgttcgc gcacagtgat gcccaggtaa
     3181 atactggcat cttgaacatg gtcatgcccg gtgcgcgcat cttcagaatg gtaacgaaga
     3241 agttgatacc ggtaagcgtc gtacctatac cggatagctg gagactccat atccagtaat
     3301 cgacaccgac tcccggactg tactctattc ccgatagcgg tggataggcc agccagccgg
     3361 tctgcgcaaa ttcgcccacg ccgagagaaa cgttaaccag aatcacacca acaacggtaa
     3421 accagaagct taagttgttg aggaacggga acgcaacgtc acgcgcgccg atctgcagcg
     3481 gaaccaccaa gttcatcaga ccgataacga aaggcatcgc cacgaagaag atcataatca
     3541 cgccgtgcgc ggtgaagatc tgatcgtagt ggtgaggtgg caggaagccc gcttcgcccg
     3601 ccgaggcaag agcctgctgg ctacgcatca taatggcgtc agcaaaacca cgcagcaaca
     3661 tcacaatcgc cacgatgata tacatgatac cgaggcgttt atggtcgacg gaggtcagcc
     3721 actctttcca caggtaggtc cacttaccga agtaagtgat caggccaacg agcgccagac
     3781 ctcccaaaat aatgccagcg atcgtaacca tgacgatagg ttcatggaac gggactgcat
     3841 caagtgataa ttttccgaac atctttattc ttcctcaacc cctttaatgg gcggattccg
     3901 cgtggctcat gtccatgcct tccatacctt cgtgtgcgct gtgctcacct tctggctggg
     3961 tcatgtccat gctcttaccg tgagccataa acttgttaat tacatcggca aacaagtttg
     4021 gtttcacgtt ggagaaatat tccacctggt tgtattcgct aggcgcggcc agtttttcga
     4081 acgctgccat gtcagacatg gagttcggcg actgtttcgc ttttgcgacc cactggtcga
     4141 atgcggcgcg atccggtgtt gcaatagctt tgaacttcat gcctgagaag cccgggccgc
     4201 tgtagctggc ggagataccg tcataagtgc cgggttcgtt ggcgatcaga tgcaggcgag
     4261 tctgcatacc ggccatggca taaatctggc tacccagacg cggaatgaag aaggagttca
     4321 tcacggagtt ggaggtcact ttgaagtaca ccggagtgtt cgccgggaaa gcgatttcat
     4381 tcacggtagc aaagccctgt tccgggtaga tgaagaacca tttccagtcc atggaaacca
     4441 cttcgatggt aatgggcttc tcgtcgtgtg ccagcggctt gctaggctca agagcgtgag
     4501 tggttttcca ggtcagtacc gcaaggaaga tgatgattaa gataggtacc gtccagacca
     4561 cagcttccac tttattggag tgtgaccagt tcgggctgta cttagcatct ttattgctcg
     4621 cacggtactt ccaggcgaaa ccaacagcca tcaagattgc gggaataacg acaatcaaca
     4681 tcaggccaaa tgccgtcagt atcagtgaac gttgctccag accaatctgt cctttgggat
     4741 ctaacagcgc agaattacag ccactgagca atacagtgcc tgcaaataat gacaaccatc
     4801 ccaaactttt attgtatttc ctgagtctca tttaacgacc tcaattccac gggatctggt
     4861 ggcgtttaaa atgtgtgggc attttacggg aaggttacat tactgtaaac atgattaaat
     4921 tcctgttact tggatttggc tgtcaggtca catatgttgc aaaacatatc aggttgattt
     4981 taagaagctg ttgcaaaagg gcgttataac aaaaggggag tgcagaagaa gtctggacgg
     5041 gcaattggta taatcaatgc gaaataaaac aattatttaa caaataatta tcattggagt
     5101 gacaacttgg tggtgagaaa ataaagactc attagctgaa tcgtgtaaga aaaatagaat
     5161 ttataaatgg cgccaataat ttccaaacgc taagccgcac aaaagaacaa atattaataa
     5221 gcgatgctga tgtttattta gccgtaataa ttacggcgag tgattactac agctaaataa
     5281 tatttacaga ttacgtcaga tgcgtttttc gtagcgccag ataatccagc aaaccaccaa
     5341 gcacgacgcc agaaagggcg actaaaaccc cgacttccag cagagcaggc aggaaagaga
     5401 agtgcgtcaa atccagcgcg tccatcgtca atagcagtaa ccacacggcc aacaggctga
     5461 cgcccgccgc cagtgtccac atggcaaagg cataacctgc cggatatgcg gtacgggaga
     5521 taaagttgtc atttactcgt gtatattcaa gcgtctggcg gcaaaccagc agcaaaataa
     5581 gccctggtac ggcagcggcg acggagaata gatagaatgt cgaccagccg tgtgcttcaa
     5641 caaaccaacc cgccacgggg ccgacataaa ctcgccctac agcagaaagc gctgagagca
     5701 gggcaaattg agtagcggaa aatgacttat tacatagcgt cattaacagc gcgacaaagg
     5761 ctgatgtgcc catcccgcca cagaggtttt cgaaaaagac ggctgcgccc atgctgtaga
     5821 gatgcttatc agtaatcgac agcagccagt aaccagcgtt agacgcacct tgtaaaatgc
     5881 cgaaaatcag cagtgcccgg aacagtgaca ggcgctgcat caaaatccca ccgtacaatg
     5941 cgccaacaat ggtcgctagt aagccaagcg ttttgtttac cacgcctact tcacccgcat
     6001 caaacccgac gccgcgaatc aaaaacgtgg ttgtcaggct catggcgaat gcgtcgccca
     6061 gcttatacag cacgataaga agcaaaataa gccaggcatt attgcgacca aagaaatctc
     6121 gcagaggtgc aacaaccgct tgttccagcg ttttgggcac aggaatggtg tcggttggtt
     6181 ctggtgcaag caacgtcgcg ataatacagg ggatcaacag cgccgccatc aaccagtaca
     6241 tgccctgcca gcccagccat ttatctgcca gccacagggc taggccgccg gaaaccagca
     6301 tccctaaacg gtaacccaga acgctgattg ccgcgcccgc accacgttct tctgccggga
     6361 gcacatcggt tttccacgca tcgaagacaa tatcctggga ggcagagcaa aaagcgatca
     6421 ccaccgccag cgccgccatc cagcggagtt gggtgcctgg ttcgagaaaa cccatcgccg
     6481 caatggcgac taataacagg atttgcgtgg cgagcagcca accgcgccgc cgcccaaaaa
     6541 atgggggcgt gtagcggtcc atcagcggtg accagaggaa tttaaaaacg taagcctggc
     6601 ctaccagaga gaagaaacca atggttttga gatcgatatt ctcgaccgtc atccaggcct
     6661 gtaaggtgcc ggatgtcagg gcgagcggta gcccggaagc aaaacccagg atcagcaata
     6721 tggctgaacg cggctgttga aaaatacgta aatattgact ggacatgggc gtctacaggc
     6781 ccggcttgca ccgggccaga gggcagaaat taacgcgcgt tctgcttgat gaattcgtga
     6841 atgctggtgt cctgagacat atcagcgatg gtatcggtca gcacactgtt aaccgcatcg
     6901 gcgatatttt tgttggaggc ctggaacgca ccttcaacgt tgtagctggc acgatagttt
     6961 ttggtcattt tgttgccatt ctgcgcggta gcgatgatgg cgatatccgc tttggtagcg
     7021 atgttgtagc gcacgttgcc ctgggacacg tcagcataca gctggctaac gatgatttgc
     7081 agattaaccg ggccattcgg accaaccatg taaccacgcg cggtcatctg tttttccagc
     7141 acttcttgca gcaggaaacg cagatcgcgg gaggcggtca gggtaacgat ttgattatcg
     7201 cgggtgactt ttgccagcgc ctgatcggta cgctgatcgg caccattaat gcttacggtg
     7261 acgcccatca ggcttggatc ctgctgtggc agtgtaatcg tcggggaaac ttcaatagtt
     7321 gttggcggtc ttgcgcatcc tgcaagcata aacagagcaa ctaacgggaa gaggattttt
     7381 ttgaacatgt tcgggctctc agagactctt aagcgtgttt ggtaaaaatt cccgccatca
     7441 taacattgcc aacggcgagg ggaagtaggt aaggcatgta aattcatcat gttgacgaaa
     7501 taatcgcccc tggtaaaaga aacgctgatg cgaggcctgt gtttcaatct ttaaatcagt
     7561 aaacttcata cgcttgacgg aaaaaccagg acgaaaccta aatatttgtt gttaagctgc
     7621 aatggaaacg gtaaaagcgg ctagtattta aagggatgga tgacatctca gcgttgtcgg
     7681 aggagatatt tcatgatgat acgtgagcgg atagaagaaa aattaagggc ggcgttccaa
     7741 cccgtattcc tcgaagtagt ggatgaaagc tatcgtcaca atgtcccagc cggctctgaa
     7801 agccatttta aagttgtgct ggtcagcgat cgttttacgg gtgaacgttt tctgaatcgt
     7861 catcgaatga tttacagtac tttagcggag gaactctcta ctaccgttca tgcgctggct
     7921 ctgcatactt acactattaa ggagtgggaa gggttgcagg acaccgtctt tgcctctcct
     7981 ccctgtcgtg gagcaggaag catcgcgtaa aaacgcattt gcaactgtcg gcgcttttcc
     8041 agtatgttgc taaagatttt atgaaaaacg gcctgcgggc cgttttgttt tgtctggatt
     8101 ttgcgctttt tgcccagcat tcagacgaaa attgcccggg aattgtgaaa aaatacgcga
     8161 cagcgcgcaa taaccgttct cgactcataa aagtgatgcc gctataatgc cgcgtcctat
     8221 ttgaatgctt tcgggatgat tctggtaaca gggaatgtga ttgattataa gaacatcccg
     8281 gttccgcgaa gccaacaacc tgtgcttgcg gggtaagagt tgaccgagca ctgtgatttt
     8341 ttgaggtaac aagatgcaag tttcagttga aaccactcaa ggccttggcc gccgtgtaac
     8401 gattactatc gctgctgaca gcatcgagac cgctgttaaa agcgagctgg tcaacgttgc
     8461 gaaaaaagta cgtattgacg gcttccgcaa aggcaaagtg ccaatgaata tcgttgctca
     8521 gcgttatggc gcgtctgtac gccaggacgt tctgggtgac ctgatgagcc gtaacttcat
     8581 tgacgccatc attaaagaaa aaatcaatcc ggctggcgca ccgacttatg ttccgggcga
     8641 atacaagctg ggtgaagact tcacctactc tgtagagttt gaagtttatc cggaagttga
     8701 actgcagggt ctggaagcga tcgaagttga aaaaccgatc gttgaagtga ccgacgctga
     8761 cgttgacggc atgctggata ctctgcgtaa acagcaggcg acctggaaag aaaaagacgg
     8821 cgctgttgaa gcagaagacc gcgtaaccat cgacttcacc ggttctgtag acggcgaaga
     8881 gttcgaaggc ggtaaagcgt ctgatttcgt actggcgatg ggccagggtc gtatgatccc
     8941 gggctttgaa gacggtatca aaggccacaa agctggcgaa gagttcacca tcgacgtgac
     9001 cttcccggaa gaataccacg cagaaaacct gaaaggtaaa gcagcgaaat tcgctatcaa
     9061 cctgaagaaa gttgaagagc gtgaactgcc ggaactgact gcagaattca tcaaacgttt
     9121 cggcgttgaa gatggttccg tagaaggtct gcgcgctgaa gtgcgtaaaa acatggagcg
     9181 cgagctgaag agcgccatcc gtaaccgcgt taagtctcag gcgatcgaag gtctggtaaa
     9241 agctaacgac atcgatgtac cggctgcgct tattgacagc gaaatcgacg ttctgcgtcg
     9301 ccaggctgca cagcgtttcg gtggcaacga aaaacaagct ctggaactgc cgcgcgaact
     9361 gttcgaagaa caggctaaac gccgcgtagt tgttggcctg ctgctgggcg aagttatccg
     9421 caccaacgag ctgaaagctg acgaagagcg cgtgaaaggc ctgatcgaag agatggcttc
     9481 agcgtacgaa gatccgaaag aagttatcga gttctacagc aaaaacaaag aactgatgga
     9541 caacatgcgc aatgttgctc tggaagaaca ggctgttgaa gctgtactgg cgaaagcgaa
     9601 agtgactgaa aaagaaacca ctttcaacga gctgatgaac cagcaggcgt aatttacgca
     9661 gcataacgcg ctaaattcgc acaaaggccc gtcaccgcca ggtggtgggc tttttttgtc
     9721 atgaattttg catggaaccg tgcgaaaagc ctctttcggt gttagcgtaa caacaaaaga
     9781 ttgttatgct tgaaatatgg tgatgccgta cccataacac agggactagc tgataatccg
     9841 tccataaggt tacaatcggt acagcaggtt ttttcaattt tatccaggag acggaaatgt
     9901 catacagcgg cgaacgagat aactttgcac cccatatggc gctggtgccg atggtcattg
     9961 aacagacctc acgtggtgag cgctcttttg atatctattc tcgtctactt aaggaacgcg
    10021 tcatttttct gactggccag gttgaagacc atatggctaa cctgattgtg gctcagatgc
    10081 tgttcctgga agcagaaaac ccagaaaaag atatctatct gtacattaac tccccaggcg
    10141 gggtgatcac tgccgggatg tctatctatg acaccatgca gtttatcaag cctgatgtca
    10201 gcaccatctg tatgggccag gcggcctcga tgggcgcttt cttgctgacc gcaggggcaa
    10261 aaggtaaacg tttttgcctg ccgaattcgc gcgtgatgat tcaccaaccg ttgggcggct
    10321 accagggcca ggcgaccgat atcgaaattc atgcccgtga aattctgaaa gttaaagggc
    10381 gtatgaatga acttatggcg cttcatacgg gtcaatcatt agaacagatt gaacgtgata
    10441 ccgagcgcga tcgcttcctt tccgcccctg aagcggtgga atacggtctg gtcgattcga
    10501 ttctgaccca tcgtaattga tgccagaggc gcaactgtgc cgctatactt atccagggcg
    10561 gcacaacgct gtaagcggct tgcgcctgag aatggcattt gcgtcgtcgt gtgcggcaca
    10621 aagaacaaag aagaggtttt gacccatgac agataaacgc aaagatggct caggcaaatt
    10681 gctgtattgc tctttttgcg gcaaaagcca gcatgaagtg cgcaagctga ttgccggtcc
    10741 atccgtgtat atctgcgacg aatgtgttga tttatgtaac gacatcattc gcgaagagat
    10801 taaagaagtt gcaccgcatc gtgaacgcag tgcgctaccg acgccgcatg aaattcgtaa
    10861 ccacctggac gattacgtta tcggccagga acaggcgaaa aaagtgctgg cggtcgcggt
    10921 atacaaccac tacaaacgtc tgcgcaacgg cgataccagc aatggcgtcg agttgggcaa
    10981 aagtaacatt ctgctgatcg gtccgaccgg ttccggtaaa acgctgctgg ctgaaacgct
    11041 ggcgcgcctg ctggacgtcc cgttcaccat ggccgacgca accacgctga ccgaagccgg
    11101 ttatgtgggc gaagacgttg aaaacatcat tcagaagctg ttgcaaaagt gcgattacga
    11161 cgtacagaaa gcgcagcgcg ggattgtcta catcgatgaa atcgacaaga tttctcgtaa
    11221 gtcagacaac ccgtctatta cccgtgacgt ttccggtgaa ggcgtacagc aggcactgtt
    11281 gaaactgatc gaaggtacgg tagctgctgt tccaccgcaa ggtggacgta aacatccgca
    11341 gcaggaattc ttgcaggttg atacctctaa gatcctgttt atttgtggcg gtgcgtttgc
    11401 cggtctggat aaagtgattt cccatcgtgt agaaaccggc tccggcattg gttttggcgc
    11461 gacggtaaaa gcgaagtccg acaaagcaag cgaaggcgag ctgctggcgc aggttgaacc
    11521 ggaagatctg atcaagtttg gtcttatccc tgagtttatt ggtcgtctgc cggttgtcgc
    11581 aacgttgaat gaactgagcg aagaagctct gattcagatc ctcaaagagc cgaaaaacgc
    11641 cctgaccaag cagtatcagg cgctgtttaa tctggaaggc gtggatctgg aattccgtga
    11701 cgaggcgctg gatgctatcg ctaagaaagc gatggcgcgt aaaaccggtg cccgtggcct
    11761 gcgttccatc gtagaagccg cactgctcga taccatgtac gatctgccgt ccatggaaga
    11821 tgtcgaaaaa gtggttatcg acgagtcggt aattgatggt caaagcaaac cgttgctgat
    11881 ttatggcaag ccggaagcgc aacaggcatc tggtgaataa ttaaccattc ccatacaatt
    11941 agttaaccaa aaagggggga ttttatctcc cctttaattt ttcctctatt ctcggcgttg
    12001 aatgtggggg aaacatcccc atatactgac gtacatgtta atagatggcg tgaagcacag
    12061 tcgtgtcatc tgattacctg gcggaaatta aactaagaga gagctctatg aatcctgagc
    12121 gttctgaacg cattgaaatc cccgtattgc cgctgcgcga tgtggtggtt tatccgcaca
    12181 tggtcatccc cttatttgtc gggcgggaaa aatctatccg ttgtctggaa gcggcgatgg
    12241 accatgataa aaaaattatg ctggtcgcgc agaaagaagc ttcaacggat gagccgggtg
    12301 taaacgatct tttcaccgtc gggaccgtgg cctctatatt gcagatgctg aaactgcctg
    12361 acggcaccgt caaagtgctg gtcgaggggt tacagcgcgc gcgtatttct gcgctctctg
    12421 acaatggcga acacttttct gcgaaggcgg agtatctgga gtcgccgacc attgatgagc
    12481 gagaacagga agtgctggtg cgtactgcaa tcagccagtt cgaaggctac atcaagctga
    12541 acaaaaaaat cccaccagaa gtgctgacgt cgttgaatag catcgacgat ccggcgcgtc
    12601 tggcggatac cattgctgca catatgccgc tgaaactggc tgacaaacag tccgttctgg
    12661 agatgtccga cgttaacgaa cgtctggaat atctgatggc aatgatggaa tcggaaatcg
    12721 atctgctgca ggttgagaaa cgcattcgca accgcgttaa aaagcagatg gagaaatccc
    12781 agcgtgagta ctatctgaac gagcaaatga aagctattca gaaagaactc ggtgagatgg
    12841 acgacgcgcc ggacgaaaac gaagccctga agcgcaaaat cgacgcggcg aagatgccga
    12901 aagaggcaaa agagaaagcg gaagcagagt tgcagaagct gaaaatgatg tctccgatgt
    12961 cggcagaagc gaccgtagtg cgtggttata tcgactggat ggtacaggta ccgtggaatg
    13021 cgcgcagcaa ggtcaaaaaa gacctgcgtc aggcgcagga aatccttaat accgaccatt
    13081 atggtctgga gcgcgtgaaa gatcgcatcc ttgagtatct tgcggttcaa agccgtgtca
    13141 acaaaatcaa gggaccgatc ctttgcctgg tagggccgcc gggggtaggt aaaacctccc
    13201 tgggtcagtc cattgccaaa gccaccgggc gtaaatatgt ccgtatggcg ctgggcggcg
    13261 tgcgtgatga agcggaaatc cgtggtcacc gccgtactta catcggttct atgccgggta
    13321 aattgatcca gaaaatggcg aaagtgggcg ttaaaaaccc gctgttcctg ctcgatgaga
    13381 tcgacaaaat gtcttctgac atgcgtggcg atccggcttc cgcactgctt gaagtgctgg
    13441 atccagagca gaacgtggcc ttcagcgatc actacctgga agtggattac gatctcagcg
    13501 acgtgatgtt tgtcgcgacg tcgaactcca tgaacattcc ggcaccactg ctggatcgta
    13561 tggaagtgat tcgcctctcc ggttataccg aagatgaaaa actgaacatc gccaaacgtc
    13621 acctgctgcc gaagcagatt gaacgtaatg cactgaaaaa aggtgagctg accgtcgacg
    13681 atagcgccat tatcggcatt attcgttact acacccgtga ggcgggcgtg cgtggtctgg
    13741 agcgtgaaat ctccaagctg tgccgtaaag cggttaagca gttactgctc gataagtcat
    13801 taaaacatat cgaaattaac ggcgacaacc tgcatgacta ccttggtgtt cagcgtttcg
    13861 actatggtcg cgctgataac gaaaaccgtg tcggtcaggt aaccggtctg gcgtggacgg
    13921 aagtgggcgg tgacttgctg accattgaaa ccgcatgtgt tccgggtaaa ggcaaactga
    13981 cctataccgg ttcgctcggc gaagtgatgc aggagtctat tcaggctgcg ttaacggtgg
    14041 ttcgcgcgcg tgcggaaaaa ctggggatca accctgattt ttacgaaaaa cgtgacatcc
    14101 acgtccacgt accggaaggt gcgacgccga aagatggtcc gagtgccggt attgctatgt
    14161 gcaccgcgct ggtttcttgc ctgaccggta acccggttcg tgccgatgtg gcaatgaccg
    14221 gtgagatcac tctgcgtggt caggtactgc cgatcggtgg tttgaaagaa aaactactgg
    14281 cagcgcatcg cggcgggatt aaaacagtgt taattccgtt cgaaaataaa cgcgatctgg
    14341 aagagattcc tgacaacgta attgccgatc tggacattca tcctgtgaag cgcattgagg
    14401 aagttctgac tctggcgctg caaaatgaac cgtctggcat gcaggttgtg actgcaaaat
    14461 agtgacctcg cgcaaaatgc actaataaaa acagggctgg caggctaatt cgggcttgcc
    14521 agcctttttt tgtctcgcta agttagatgg cggatcgggc ttgcccttat taaggggtgt
    14581 tgtaagggga tggctggcct gatataactg ctgcgcgttc gtaccttgaa ggattcaagt
    14641 gcgatataaa ttataaagag gaagagaaga gtgaataaat ctcaattgat cgacaagatt
    14701 gctgcagggg ctgatatctc taaagctgcg gctggccgtg cgttagatgc tattattgct
    14761 tccgtaactg aatctctgaa agaaggggat gatgtagcac tggtaggttt tggtactttt
    14821 gccgttaaag agcgtgctgc ccgtactggc cgcaacccgc agaccggtaa agagatcacc
    14881 atcgctgccg ctaaagtacc gagcttccgt gcaggtaaag cactgaaaga cgcggtaaac
    14941 taagcgttgt ccccagtggg gatgtgacga agttcaaggg cgcatctact gatgtgcctt
    15001 ttttatttgt attcggtgac tttctgcgtc ttgtgggctg acaattgccc ccgtttcttg
    15061 tcacaatagg cctttgcgcg catcgatacg ttgcgtgagg tacacagtca tctacagcgg
    15121 agtgttgtta caccatgatg gacagcttac gcacggctgc aaacagtctc gtgctcaaga
    15181 ttattttcgg tatcattatc gtgtcgttca tattgaccgg cgtgagtggt tacctgattg
    15241 gcggaggcaa taactacgcc gcaaaagtga atgaccagga aatcagccgt gggcaattcg
    15301 agaatgcctt caacagcgag cgtaatcgca tgcagcaaca gctgggcgat caatactccg
    15361 agctggcagc gaacgaaggc tatatgaaaa ccctgcgtca acaggtgctg aatcgtctga
    15421 tcgacgaggc gctgctggat cagtacgcac gtgagctgaa actgggtatc agcgatgagc
    15481 aggttaaaca ggcgattttc gcgaccccag ccttccaggt tgatggcaaa tttgataaca
    15541 gccgctataa cggtatcctc aaccagatgg ggatgaccgc cgatcagtac gcccaggcgc
    15601 tgcgtaacca gctcactacc caacagctga ttaacggcgt tgctggtacc gattttatgc
    15661 tgaaaggtga aaccgacgag ctggcggcac tggtcgctca acaacgcgtg gtgcgtgaag
    15721 cgactatcga tgttaacgcg ctggcggcga agcagcctgt gaccgaacag gaaattgcca
    15781 gctactacga acaaaacaaa aacaatttca tgacgccgga acaattccgc gtgagttaca
    15841 tcaagctgga tgccgcaacg atgcagcaac cggttagcga tgcggatctc cagagctact
    15901 acgatcagca tcaggatcaa ttcacccagc cgcagcgtac ccgttacagc atcatccaga
    15961 ccaaaactga agatgaagcg aaagcggtac ttgatgagct gaataaaggc ggtgattttg
    16021 ctgcattagc caaagaaaaa tctgccgata ttatctctgc tcgtaacggc ggcgatatgg
    16081 gttggttaga agatgccact atcccggatg aactgaaaaa tgctggtctg aaagaaaaag
    16141 gccaactgtc tggtgtcatc aaatcttcgg tcggtttcct gattgtacgt ctggacgaca
    16201 ttcagccagc gaaagtgaaa tcgttagacg aagtacgtga cgatgtcgcg gcgaaagtga
    16261 aacacgaaaa agccctcgat gcgtactacg cgctgcagca gaaagtgagc gatgcggcaa
    16321 gcaacgacac cgagtctctg gccggtgcag agcaagctgc cggcgttaaa gccactcaga
    16381 cgggttggtt cagcaaagag aacctgccgg aagagttgaa cttcaagccg gttgctgacg
    16441 ctatctttaa cggcggtctg gtaggtgaaa acggcgcgcc gggcatcaac tctgacatca
    16501 tcaccgtaga cggcgaccgc gcattcgtgc tgcgcatcag cgagcacaaa ccggaagcgg
    16561 tgaaaccgtt ggcagatgtt caggaacaag ttaaggcact ggttcagcac aacaaagctg
    16621 aacaacaggc gaaagtggat gctgagaaac tgctggttga tttgaaagcc ggcaaaggtg
    16681 cggaagctat gcaggctgcc ggtctgaaat ttggcgagcc gaaaacctta agccgttccg
    16741 gtcgtgaccc gattagccag gcggcgtttg cactgccact gccagcgaaa gacaaaccga
    16801 gctacggtat ggcgaccgat atgcaaggta atgtggttct gctggcgctg gatgaagtga
    16861 aacaaggttc aatgccggaa gatcagaaaa aagcgatggt gcagggtatc acccagaaca
    16921 acgcacaaat cgtctttgaa gctctgatga gtaacctgcg taaagaggcg aaaatcaaaa
    16981 ttggcgatgc gttggaacag caataatcct gaagccgcct cgcaaaaaaa tgctttgcag
    17041 ctgtaacaag aaaaggtcgc tttcgcggcc ttttccattt ctgaacattg ccatttgttt
    17101 actgttttca ctgccgttaa ggtgattcca ctgttaacaa acaaggagaa aacagtatga
    17161 aacacggaat taaagcactg ctcattaccc tgtccctggc ctgtgccgga atgtctcata
    17221 gcgcgctggc ggcagcttct gtggcgaaac cgacggcggt agaaaccaaa gcggaagctc
    17281 ctgcagcaca aaataaagca gcagtaccgg cgaaagccag tgacgaagaa ggctcccggg
    17341 tcagcattaa taatgccagc gcggaagagc tagcccgcgc gatgaatggc gttggcctga
    17401 agaaagcgca agcgattgtc agttatcgcg aagagtacgg tccgtttaaa acggtggagg
    17461 atctaaagca ggtgccgggg atgggcaatt cgctggtgga acgtaatctg gcggtattaa
    17521 ccctgtaatt aatttgcata gtggcaattt tttgccagac tgaagaggtc ataccagtta
    17581 tgacctctgt acttataaca gtttttataa aaacaacgta aggttattgc gctatgcaaa
    17641 cacaaatcaa agttcgtgga tatcatctcg acgtttacca gcacgtaaac aacgcccgct
    17701 accttgaatt tctcgaagaa gcccgctggg atgggttgga aaatagcgac agttttcact
    17761 ggatgacggc ccataacatc gctttcgtgg tggtgaatat caatattaac tatcgtcgtc
    17821 cggcggtatt aagcgacttg ttaaccatta cgagccagtt acagcaatta aacggtaaaa
    17881 gcggcatctt aagccaggtc attacactgg agccggaagg gcaggtggta gcggatgcgc
    17941 ttattacgtt tgtttgtatt gatcttaaaa cgcagaaagc attagctctg gaaggggaat
    18001 tgcgcgaaaa gctggagcag atggttaagt aaacgttttg tggtgccgga tgctcaagcc
    18061 gcatccggcg acacccggaa taattacttc aacccggttt tctgcttcat cgctgccatc
    18121 accgtcggtt tatcggccag ataatgattc aaaccgttgg cgcgtaaatt acatgccgca
    18181 caatgaccgc aaccgtcgcc tttaatgccg ttatagcagg tcaacgtttc gttacggact
    18241 aaatccagtt tgccgtaata atctgccagc gcccaggttt ccgctttatc aatccacatc
    18301 agcggcgttt caaaacgaat atctttcgcc atgcccaaac tgacggcatg gtttagtact
    18361 ttcacaaact catcgcggca atccgggtag ccggagaaat ccgtttcgca gacgccggta
    18421 attacggctt ctgcttttac ctgatacgca tatattgccg ccagcgtcag gaacaaaata
    18481 ttacgccctg ggacaaacgt attcgggatg ccatcggctt caggttcata atcaggcacc
    18541 ggaatgctgt cacgcgtcag gctgctgacc gccagctcgt tgagcagcgt tacatcaaga
    18601 accttatgcg cgcgtgcccc cagtttcagc gccagttcgc gtgccacgtc gatttctgcg
    18661 cgatggcgct gaccgtaatc gaacgtcacg caatggactt catcatattg ttgtaatgcc
    18721 tgcaccagac aggtggtgga atcctggcct ccactgaaca caacgacagc acgtttcata
    18781 gataatcctg cttgaacaat aaaagcgtta tggtaacgcc tgcgattaac ccggaccagc
    18841 tattcattgc gacggcgcgg gaagccaggc ttcggtaaat tcaaaccagc cgcgcggtgt
    18901 cagtcgcaca ccgttcacgc cgggaggggc actaatgcga tagtgatagt tgaacagcgg
    18961 cgtcagcgtc gcatctgtca ttaactggct aaaaaccgct ttcagggcat taaatcggtt
    19021 ttcctcatca ggcattactt gcaccgcgtc cagtgtcgat tgtagatgtg cgtatgctgg
    19081 agcgtcgaaa acatgtggcc acagcggatc gcagcgcagc cattgctcca gagtatattc
    19141 cggtgcttcg ccaattaatc tgtcgcccat catgaggtct gcgtgtgcca gtagggtcgt
    19201 gtcgtcccag ttttttgcgt tatgaaaaat aattgtgagt tcacagcctt ccgctgccag
    19261 tgtcgcctgt aggcgttctg ccatggtatg aagttctatc gggaggtgat aaaccagcgt
    19321 caaggttttc ggtagtttga cttcatccgg tacttgccaa tgcggaatag tccagcctgg
    19381 cagtaatgca tgactggcgg tgatcaggtt ttctccgact tctaacgttt gtaataaacc
    19441 ggattgatga ataatggaga tcacttttcg cgcctgccag agggagagtc ggggactttt
    19501 gcgcaacgtt aaatagcaaa aacctaaact gatgccacta ctgacctggc tgacccgttg
    19561 cagctcctcc ggtttgccga tggtgatttg cacgggatgc cgacaactgg ttcccaaatc
    19621 tttttcgaaa agcggcggag ttatccagta ctcaaccgct ttaagcagcg gatgacgtaa
    19681 atggtaataa tcatggcttt ccaggcgcac cagttcagct gtgaattgtg ttaagcgaaa
    19741 aggacccgtg ccaatcagtg ggaattgcgg atgcgccaga tggctgcaat agctcgccag
    19801 ccggtgcgca agccagtaat cggggcgatg taaaaagaag gtcagacact gcggatgggt
    19861 gacctcaata cgcttcacgc taataaataa ttgatccagt gctggcagtt gtaacagcat
    19921 caataatcgc tggtgtaagt gtgaggcttt tactgcatcg ccgttatgcc agtgtagggt
    19981 tgaacgaaga taaaagtccc agcgtaaccc gtcagcagag gtttcccagt gatgcgctaa
    20041 atcgccaatc gggcgctggg tattattatc gaagcgggtc aggccggaaa atatctgtcc
    20101 ggcgagatgc tgctcggcac ggccgggcaa aaagcccggt tgtagcggtt cgagcgggcg
    20161 atagtaggga atacgcaacg tgggtgtatc gttttgccat tgtccgccca taaacggctg
    20221 taacagagtg cgcagctcac ctggggccag ttgcgccagc tccagcacat cttgctgctt
    20281 tccggtttcc agtgcctttt ccatcatcgc attgcgtagc gattctggcg tgaccagaaa
    20341 gcgtaattgt ccgcgctttc cgcgtcctga ctgcgcctgc cactccagcc atcccgcttc
    20401 ctgtgcctga cgcaacagcg tacgaacatg gcgttcgctg caaaaacagc gttcggccag
    20461 ttcgctgacg gtgacggttt gcggctttcc ggcggaaggt tgccacagac gttgatactg
    20521 gttaaggcgg ttgagcagtc gcataaaaac ccggaacaat attatttaac tattcactat
    20581 tacttccgta tatatcaggt gatactcaat caccattaac cgtgtcacag agtggagaaa
    20641 gaaatggctc gtctggcagc atttgatatg gatggcactt tattgatgcc tgaccatcat
    20701 ttaggtgaga aaaccctctc tactttggcg cgactgcgtg aacgcgacat taccctcact
    20761 tttgccacgg ggcgtcatgc gctggagatg cagcatattc tcggggcgat atcgctggat
    20821 gcgtatttga ttaccggcaa cggaacgcgc gtgcattctc tggaaggtga acttttacat
    20881 cgtgatgatt tacctgcgga tgtcgcggag ctggtgctgt atcagcaatg ggatacccga
    20941 gccagcatgc atattttcaa tgacgacggt tggtttaccg ggaaagagat ccctgcgttg
    21001 ttgcaggcat ttgtctatag cggttttcgt tatcaaataa tcgatgtcaa aaaaatgcca
    21061 ctcggcagcg tcaccaagat ctgcttctgt ggcgatcacg acgatcttac acgtttgcag
    21121 atccagctac acgaagcatt aggcgagcgt gcacatttgt gtttttccgc cacggattgc
    21181 cttgaagtgc tgccggtggg ctgcaataaa ggcgctgcac tgacggtgct gacccaacat
    21241 ttaggtttat cgttgcgcga ttgcatggcc tttggtgatg cgatgaacga tcgcgaaatg
    21301 ttaggcagcg tcggtagcgg atttattatg ggcaatgcga tgccgcaact gcgcgcggag
    21361 ctcccgcatt taccggtgat tggacattgc cgaaatcagg ctgtctctca ctatttgacg
    21421 cactggctgg actatccaca tctaccttat tcccccgaat aacgagatcc cttccagcac
    21481 cgggcaattg cccggttttt ttgcgttgaa tttgtcattt tgtgccgtgg tgtttaaacc
    21541 gcacagaata aattgtcgcg atttcacctt taaaatagaa ttaaaagaga aaaaaattct
    21601 ccgtggaagg gctatgttag ataaaattga ccgtaagctg ctggccttac tacagcagga
    21661 ttgcaccctc tctttgcagg cactggctga agccgttaat ctgacaacca ccccctgctg
    21721 gaagcgcctg aaacggctgg aggacgacgg tatccttatc ggcaaagtcg ccctgctgga
    21781 tccggaaaaa ataggcctcg gcctgaccgc ctttgtgctg ataaaaacgc aacatcacag
    21841 cagcgaatgg tattgccgct ttgtcacggt ggttaccgaa atgccagaag tgctggggtt
    21901 ctggcgcatg gctggtgaat acgattatct gatgcgcgtc caggttgccg acatgaaacg
    21961 ctacgacgag ttttataagc gtctggtaaa cagcgtgccg gggctgtcgg acgtcacttc
    22021 cagcttcgcg atggaacaga ttaaatacac cacttcttta cccatcgaat aaatatccag
    22081 aatcaggtca ggacacaacg cgtgcgatta tttgctcaat taagctggta tttccgtcgg
    22141 gaatggcgtc gctatctcgg ggctgtcgcc ttgcttgtca ttatcgcgat gctgcaactg
    22201 gttccaccaa aagtggttgg tattgttgtc gatggcgtga cagaacaaca ctttactacc
    22261 gggcagatcc tgatgtggat cgccaccatg gtgctgattg ccgttgtggt ttatctcctg
    22321 cgttacgtct ggcgcgtatt gctgtttggt gcgtcttatc aactggctgt tgaactgcgt
    22381 gaagattatt accgtcagct aagccggcag catcctgagt tttacctgcg tcatcgcacg
    22441 ggtgacctca tggctcgtgc gactaatgac gtcgatcgcg tcgtgtttgc cgccggagaa
    22501 ggggtgctga cgctggtgga ttcactggta atgggctgcg ctgtgttgat tatgatgtct
    22561 acgcaaatta gctggcagtt gaccttattt gccctgttac cgatgccagt gatggcgatc
    22621 atgattaagc gcaacggcga tgctttgcat gaacgcttta agctggcaca ggcggcgttt
    22681 tccagtctta atgaccgcac ccaggaaagc ctcaccagta tccgcatgat caaagccttt
    22741 ggtctggaag atcgccagtc ggcgttattt gccgcggatg ccgaagatac cggcaaaaaa
    22801 aacatgcggg tggcgcgtat tgatgctcgt ttcgacccga ccatctatat cgcgattggt
    22861 atggcgaact tgctggcgat tggcggcggt agttggatgg tggtgcaggg cagtttaacg
    22921 ctgggccagc tcaccagttt tatgatgtat ttaggtctga tgatttggcc aatgctggcg
    22981 ctggcatgga tgtttaacat tgtggaacgt ggtagtgctg cgtacagccg tattcgcgcg
    23041 atgctggcgg aagcgccggt ggtgaacgat ggtagcgaac ccgtgccgga agggcgtggc
    23101 gaactggatg taaatattca ccagttcacg tatccgcaga ctgaccatcc tgcgctggaa
    23161 aacgtcaatt tcgccctgaa acccggtcag atgctgggta tctgcgggcc gactggttcc
    23221 ggcaaaagta ccctgttgtc gctcattcag cgtcatttcg acgtcagcga gggggatatt
    23281 cgctttcatg atattcctct gacgaagtta caactcgata gctggcgtag ccgcctggcg
    23341 gtagttagcc agacgccatt ccttttttct gacaccgtgg cgaataacat cgcgctgggt
    23401 tgcccgaatg ccacccagca agagattgag catgtcgcgc ggttagccag cgtacatgac
    23461 gatattttgc gtctaccgca aggttacgat acagaggtgg gcgagcgcgg tgtgatgctt
    23521 tccggcgggc aaaaacagcg tatctccatt gctcgtgcgt tattagtcaa cgcggaaatc
    23581 ctcatccttg atgatgcgct ttcggcggtg gacggacgca ctgagcacca gatcctgcat
    23641 aacctgcgtc agtgggggca gggaagaacg gtaatcatca gtgcccatcg cctttctgca
    23701 ctgacggaag ccagtgaaat tattgtgatg cagcacggac atatcgccca gcgtggcaat
    23761 catgatgtgc tggcacaaca aagcggctgg tatcgcgata tgtatcgcta tcaacaactg
    23821 gaggcggcgc tcgacgacgc tccggaaaat cgcgaggagg ccgtcgatgc gtagttttag
    23881 ccaactgtgg ccgactctca agcgcctgtt agcgtacggt tcaccgtggc gtaaaccgct
    23941 ggggattgcg gtcctgatga tgtgggttgc ggcggcggca gaagtcagtg ggccgctgct
    24001 tatcagctat tttatcgaca atatggtagc gaaaaataac ctgccgttga aagtggttgc
    24061 agggctggct gcggcgtatg ttgggctgca actgtttgcc gccgggctac attacgcgca
    24121 gtcgctgctg tttaatcggg cggcagtagg cgtagtgcaa cagttgcgta ccgacgtgat
    24181 ggatgctgcg ttacgccagc cattaagcga gtttgatacc caacccgtcg ggcaggtgat
    24241 ttcccgcgtc actaatgaca ctgaagtgat ccgcgatctc tacgttaccg tagtggcaac
    24301 tgtcctgcgc agtgccgcac tggtgggcgc gatgctggtg gcgatgttca gcctcgactg
    24361 gcgaatggcg ctggtggcga taatgatttt cccggtggtg ctggtggtaa tggtgatata
    24421 ccagcgttac agcacgccaa ttgtccgtcg tgtgcgcgcc tatctggcgg atatcaacga
    24481 cggctttaac gaaatcatca atggcatgag cgttatccag cagtttcgtc agcaggcgcg
    24541 atttggcgaa cgtatgggtg aggccagtcg ttcacactat atggcgagga tgcaaaccct
    24601 gcgcctcgac ggttttctgc tgcgtccgct gctgagtctg ttttcatcgc tcattctctg
    24661 tggcttgttg atgctgtttg gcttctccgc cagcggcacc attgaagtgg gcgtgttgta
    24721 tgcgtttatc agctatcttg ggcgacttaa cgaaccctta atcgaactga ccacgcagca
    24781 ggcgatgctg caacaggctg ttgttgctgg tgagcgcgtg tttgagctga tggacggacc
    24841 gcgccagcaa tatggcaatg atgatcgccc gttacagagt ggcaccatcg aagtcgataa
    24901 cgtgtcattt gcttatcgcg atgacaatct ggtgctaaag aacattaatc tctctgtgcc
    24961 ttcgcgtaac tttgtggcgc tggtcgggca taccggcagt ggtaaaagta ccctcgccag
    25021 tttattgatg ggctattacc cgctaacgga aggtgagatt cgccttgatg gtcgtccatt
    25081 aagttcgcta agtcacagcg cgctgcgcca gggcgtggca atggtgcagc aagatccggt
    25141 ggtgctggcg gataccttcc tcgccaacgt gacgctgggg cgggatatct ccgaagaacg
    25201 cgtctggcag gcgctggaaa ccgtgcaact ggcggagctg gcacgcagta tgagcgacgg
    25261 gatttatacg ccgctgggcg agcaggggaa taatctctct gtcgggcaaa agcaactgct
    25321 ggcactggcg cgcgtgctgg tcgagacgcc gcaaatcctg atccttgatg aggcaaccgc
    25381 cagcattgac tccggtactg aacaggcgat tcaacatgct ctggcggcgg tgcgtgaaca
    25441 taccacgctg gtagtgattg ctcaccgctt atcgactatt gttgatgccg acactattct
    25501 ggtgcttcat cgtgggcaag ccgtggagca gggcactcac cagcaactgc tggcggccca
    25561 gggacgctac tggcagatgt atcaactgca acttgcgggc gaagagcttg cagccagcgt
    25621 gcgtgaagaa gaatcattga gcgcctgaat agcgcaatat ctcatcgttg gtgcaaaaat
    25681 gtaacgcact gtgcactgtc atagtgcgtt ttcattttca atcttcttaa cttcctgctc
    25741 tctttctcgt ttttcatttc tggcacaccg cttgcaatac cttcttcgtg tagcagaacc
    25801 attaccgaat tctgaccgga ggggatctat gaagctggtg accgtgataa tcaaaccatt
    25861 caagctggaa gacgttcgtg aagcgttatc ttccattggt attcagggcc tgaccgtcac
    25921 cgaagtgaaa ggtttcgggc gtcagaaagg gcatgccgag ctgtaccggg gggcggaata
    25981 cagcgtcaac ttcctgccaa aagtaaaaat tgatgtggcg attgctgatg accaactcga
    26041 tgaagtgatc gatatcgtca gtaaggcggc ttacaccgga aaaattggcg acggcaaaat
    26101 cttcgtcgct gaattgcaac gcgtcattcg tattcgtacc ggcgaagccg acgaagcggc
    26161 gctgtaatct ctggcacaca gcaacaggaa cgaaaaatga agatagcgac gataaaaact
    26221 gggcttgctt cactggcgat gctgccggga ctggtaatgg ctgcacctgc ggtggccgat
    26281 aaagccgaca atgcgtttat gatgatttgt actgcactgg tgctgtttat gactattccg
    26341 gggattgccc tgttttacgg tgggttgatt cgcggcaaaa acgtgctgtc gatgctgacg
    26401 caggtgacgg tgacgtttgc actggtctgt attctctggg tggtttacgg ttactcgctg
    26461 gcgtttggtg agggcaacaa cttcttcggc aacattaact ggttgatgct gaaaaacatc
    26521 gaactgacgg cggtgatggg cagcatttat cagtatatcc acgtagcgtt tcagggatcg
    26581 tttgcctgca ttaccgtagg cttgatagtt ggggcgctgg cggaacgaat ccgcttctca
    26641 gctgtgttga ttttcgtggt ggtatggctg acgctctctt acattcctat tgcgcatatg
    26701 gtgtggggcg gtggtttgct ggcttctcac ggtgcgctgg atttcgcggg tggcacggtg
    26761 gtgcatatta acgccgcaat cgccggtctg gtgggcgcgt atctgatagg caaacgcgtg
    26821 ggcttcggta aagaggcgtt taaaccgcac aacctgccga tggtcttcac cgggactgcc
    26881 attctctata tcggttggtt tggctttaac gccgggtcag cgggcacggc gaatgaaatc
    26941 gcggcactgg catttgtgaa tactgtggtc gcaacggcgg cggcaattct tggctggatc
    27001 ttcggtgaat gggcgctgcg tggtaagcct tcactgctgg gagcgtgttc tggcgcgatt
    27061 gccggtctgg tcggcgtgac gccagcgtgt ggctacattg gtgttggcgg cgcgttgatt
    27121 atcggcgtgg tagctggtct ggcgggcttg tggggcgtta ccatgctcaa acgcttgctg
    27181 cgggtggatg atccctgcga tgtcttcggt gtgcacggcg tttgtggcat tgtcggctgt
    27241 atcatgactg ggatttttgc cgccagctcg ctgggcggcg tgggcttcgc tgaaggtgtg
    27301 acgatgggcc atcagttgct ggtacagttg gaaagcattg ccattacgat tgtgtggtct
    27361 ggtgtggtgg catttatcgg ctacaaactg gcggatctga cggttggtct gcgtgtgccg
    27421 gaagaacaag agcgagaagg gctggacgtc aacagtcacg gcgagaatgc ctataacgca
    27481 taacaagcac tgcaaaaaac agccggacgg ttttcacctc cggctatttt tttaattgtg
    27541 attacgcatc accccttcct gaacggtcga ggcaaccagt acgccgtcct gggtataaaa
    27601 ctcaccgcgc acaaagccac gtgcgctgga cgccgaggtg ctctccacgc tatacagcag
    27661 ccattcattc aaattaaacg ggcgatggaa ccacatggaa tggtcaatgg tggcaatctg
    27721 aatccccggt tcgagaaaac cgataccgtg cggctgtaga gctaccggca ggaagttaag
    27781 atcagaagcg taaccgagca gatactgatg aacgcgcagg tcatccggca cgctaccatt
    27841 tgcgcggatc cacacctgac gatgtggttc tgcgacgtga cctttcagtg ggttatgaaa
    27901 ctccaccgga cggacttcca gcggacgatc gcagatgaat ttatctttaa gcactggcgg
    27961 cagcaggtgc gccagcgatt gggcgatttg cgtttccgaa ggcaggccat caggcgctgg
    28021 cgcggacggc attgtttttt gatgttcgaa acccgcttct ggtgcctgga aagaggccgt
    28081 catataaaaa atcggtttgc cgttttgaat agcagcaacc cggcgggcgc tgaagctgtt
    28141 accgtcacgc agcgtttcga catcataaat aatcggcttc ttactatcgc cagggcgaag
    28201 aaagtagctg tgaaacgaat gtaccagccg ctcttcaggg acggtctctt ttgcagcata
    28261 caaggcctga cccacgacct ggccgccaaa tacctggcgt aaacctaaat cttcactctg
    28321 gccgcgaaag agtccttcct caattttttc cagatttaac aatgtcagta aattttttag
    28381 cgcctgactc atataactct ccagtaacaa agctgccgca gcaagccaaa gtgagttgag
    28441 tataacgcaa atttgctact ggtccgatgg gtgcaatggt ctgaattacg ggctaattac
    28501 aggcagaaat gcgtgatgtg tgccacaatt gttgacgtta ctattttgtt aaccactctt
    28561 ccggcgagga aagttagccc gctggtgcat tgataataag gagaaatgaa tgaaactcgt
    28621 gcacatggcc agtggtttag cggttgcgat tgcgttggcg gcttgcgcag ataaaagcgc
    28681 ggatattcag acgccagccc cggctgcaaa tacgtctatt tcagcaacac aacaacctgc
    28741 tatccagcaa ccgaatgtct ccggtaccgt ctggatccgt cagaaagtcg cactgccgcc
    28801 tgatgctgtg ctgaccgtga cactttctga cgcgtcgtta gccgatgcac cgtcaaaagt
    28861 gctggcgcag aaagcggtgc gtactgaagg taaacagtca ccattcagct ttgttctgcc
    28921 atttaacccg gcagatgttc agccgaacgc gcgtattctg ttgagtgcgg cgattaccgt
    28981 gaatgacaaa ctggtattta ttaccgatac cgttcagccg gtgatcaacc aggggggaac
    29041 caaagccgac ctgacattgg ttccggttca gcaaaccgcc gtgcctgtcc aggccagtgg
    29101 tggcgcaaca actaccgtac catcgacttc accgacccag gtgaatccat cttcggcagt
    29161 tcctgcccct acgcaatact aagcgttact aacccctttc catctggaga ggggttagta
    29221 gttccagcga tagcgctgta agtcgatttg cccgcttccc gataccatca caccttctgc
    29281 cagtaatgcc tgtcgttgac gctgtaaatc cggtccggtt agcgaaatgg tgccgtggcg
    29341 attaaccacc cggtgccagg gcaaggtgct gccttcgggg agacgcttta acacaccgcc
    29401 aacctggcgc gcggcgcggg gcgatcccgc cagtttcgcc acatcaccgt aagtggtgac
    29461 atacccttcg ggaatagcgg ctacgatttg ccagacgcgt tggggaaatg aatcttcttt
    29521 ttccatcttt tcttcctgag ataatttttc agcataatct ggaaaaacgc ccgagtgaag
    29581 ccgcattgcg caagaaacca gcatctggca cgcaatgggt tgcaattagc cggggcagca
    29641 gtgataatgc gcctgcgcgt tggttctcaa cgctctcaat gggggctctg ttggttctcc
    29701 cgcaacgcta ctctgtttac caggtcaggt ccggaaggaa gcagccaagg cagatgacgc
    29761 gtgtgccggg atgtagctgg cagggccccc acccatttct gcctcccacc gtttcgtcaa
    29821 aaaatcccaa catgactaaa ctttaaccac aactgacgtc gcaagaattg tctggctgcg
    29881 cagtacgctt cggaggtatg tctgatgaag tatgttgatg gttttgtggt tgccgttcct
    29941 gccgataaaa aggatgccta tcgggaaatg gccgctaagg ccgcgccatt gtttaaagag
    30001 tttggcgcgc ttcgtattgt cgaatgctgg gccagcgatg taccggatgg caaagtgacc
    30061 gattttcgta tggcggtgaa agcggaagag aatgaagagg ttgtctttag ctggattgaa
    30121 tacccttcaa aagaggtccg cgacgcagct aatcaaaaga tgatgtcgga cccacggatg
    30181 aaagagttcg gcgagtccat gccgtttgac ggcaagcgaa tgatctatgg cggattcgag
    30241 tcaatcatcg acgaataggt agcgtgacgg gctgtgcggc gtgccgtcag cccaaaaatt
    30301 cacaaatgtt gctcggccca gcgtaagaaa tcttctttcg gtaatgcctt gctgtagagc
    30361 cagccctgac cgtagtgcac gccatgctgg cgtagccact cttcttgttt actggtttcg
    30421 attccctccg ctaccatttt cagtttcagt gtttttgcca tttcgatgat atgcggcgtg
    30481 acatttttat attccagcgc atcaacgaaa gatttatcga tcttcagaat gtcgacatcc
    30541 agatcctgta aataacttaa acttgaatac cccgtaccaa aatcatcaag ataaatttca
    30601 tggcccgcct cccggtagcg agaaattatc ggggcgctgg ttttcggatc ggcaaactcg
    30661 cgttcagtga gttcaagcgc gatctgtctg ggattaacct gatagtgatt gatcatttca
    30721 cgcagcaatt gcgggatttt ttccgaggtg agcacggtgg attcaagatt gatcgaaata
    30781 tgctgctgtg gatgctgacg cagccagtcg cccatatctt caaagacgct tcttataatc
    30841 agtagcgtca atggctcaga aaggcccgtt tgctgtgcca gcggaataaa actatctggt
    30901 gacaaccaac taccgtctgt ctgcggccag cgcgccagtg cctcagcacc gacaattttg
    30961 ccattggcta aggagacaat cggctgatag tgcacgcaaa tatcacgatt ttcgatagca
    31021 tcctgcagcc gatgatgcgg tgactggatg cggcgcaaaa tgcgcagcac aaacatcgct
    31081 gccagcaggc caatcaccaa cccgagcggt aaccaaataa agacttgccg atgccagcct
    31141 ttctgcaaca ttttcgatga agcccaggtg ataattgaaa tattcatctc cgggaagggc
    31201 aggatatcgt aaatgattcc attattttcg atatgctcac cgggtgtttt ttgtagcctg
    31261 gtaataattc cctgagcaat ttcatcgctg ctggttatga caacgttatg ggcattgcca
    31321 ataatggcgg catcaatttg ccatgagcta tagggaatga catcaataaa ggaagcgggg
    31381 tcgatcatga cgacataatg tgccgttccc atggcgacca tgtaacggat aatgcctaaa
    31441 tcgttatgtg atgttaacca gacacgataa ccatctttcg aaattttacc tggctcgggg
    31501 aaggtatcgg gcggactttc atgctccaga gacgaacact ggggaacgtt gttatcgata
    31561 taaaccactt cctgaatata gcgataactg taagataccc gacgcatttc catgagatgg
    31621 gcttcactac agtcagcgcc ttgccatctt tccagctctt gcagcgcatc tttcccttgt
    31681 gtcgccacct tattggcccg aatagcgacg cgggaggaat aggtatccag ctcttcaata
    31741 aacgatgttt ctacctgctg atgggccagc cagatgctta agccgacagg cagcaatact
    31801 gaaagaatca gtactcccga aataaggccg accagatgtc gtgttctcac cagcatatcc
    31861 ttattaacac agtgtaggga aagtatatct gattgtggaa aaaggctggc aggagataag
    31921 gaggtagagc tggtatttca tgaagtgatg ggtggaaacg atcgttgtca tttgaccagg
    31981 cgcagagctt tacgcctggt atgtaatcct aacttaagat gttgattcgg cgcgagcgag
    32041 ggtaaaaata tcgtaaaaag acaattcacc tttacggacg atcatttttt gcagttgttc
    32101 cttatgaaca tccgcgacac gcggagagtt caagatggct tcaaccagcg cggcaggtac
    32161 aaagcgcgtg ttgtaccatt cgccgttata gcaaacgcgg aaatccaaca cgtcgatatc
    32221 ttcatagcgg tagcgtgggt agacatcgaa aaagaagaaa ccattaagca ggataaataa
    32281 tacaaccagt agccagacag agcctgaaag cgtttcggac tgcaacatca ccgccagggt
    32341 ggcaaaaaaa gcaacgtaca taccgataaa cagccccgga tgtttacgga taaaactgat
    32401 actaaagcgg ggtttgttgt cgcgtttttc gcgggtattc agagactcaa tcgtttcggt
    32461 cagcaggcgt tgtatttcgg tcatcgtttg ccttcgtggt atctgcaact ttccagggaa
    32521 caccctattt taacgtggga atagtagagg gaaagtaaca gatctgccac aattttgcat
    32581 aacagttgcg aaaaaccgat tacaattttt taattattct ggctggatta ccgcccacga
    32641 caacgttgtc cgggacatct tttgtgacaa ctgcacctga ggctaccacg acgttatcac
    32701 caatggtcac accagggtta atgaccgcgc gtccgccaat ccagacgtta ttaccgatgg
    32761 tgacgggttt ccccagttca gcaccgctat tacgtgctac agggtcgatg ggatgtgttg
    32821 ccgtgtagat atgaacgcct agtgccaaca tacagttatc accgatgcga atagggcaga
    32881 catcaagcat cacgcaatcg aagttggcga aaaaattatt accgagaaaa atgttatagc
    32941 catagtcaca gcgaaacgtt ggctcaatat aagcctctgt cacctgaccg aatagatcag
    33001 cgagaatttg ctggcgtaat gtgtgctctt ccgccaggga atgattgtat cggtgaataa
    33061 gctgacgagc gcgcaggcga tcgcgagata acgtctcatc tgccgagcga tacaactcac
    33121 cagcaatcat cttttctttt tctgtgctca ttactgacct ccggttgctg aaaatgcggc
    33181 aacacagtaa gacgaaaagt cagtgaagag aacgttccca ttacatctgt gtgataaaga
    33241 tcacataggg tatgtcttca tggcgaaaaa gtataaaatt cttaataaac agccggttat
    33301 agctccgaaa gcgaattatt tattagcgaa taaatttcca tactgaggaa gggatcttgt
    33361 cgtacagttt attcatggtc aattcggcga ggcggtgatc tgcggctgag taaaataccg
    33421 ccagttcatt atctgataat tcgtatttat ttttctcgat aacacgctcc agcgtgtcaa
    33481 ttgtctggca acgacgtaaa cgcattaaat aatcggtttt cgttaaaggt ttttcggaca
    33541 tacttctacc catggttgta ataattctaa caagataaac tcgcaggatt ctctttcgtc
    33601 gcattgacaa aacaacggaa tagtcgatta cctgacttcc gccatttctg aagatcctgc
    33661 atattaatac cataactact gaacaacata aaggtgtcat ccagatattc gtcgatctgc
    33721 tcaatgagct tattgtcttc attatactta attttgtaat taagtgcgaa ggtcgcaata
    33781 tgctcaatca gttcattcaa ctggaggttg atcgccgagg ttgggtcgtt aacccagcca
    33841 tgattgcttt cttcaaggtt tgcaaggcag tcatgataca gagtttcaca gagaaactta
    33901 agctgtgcga tatcatgtct tttgggtgag tattcatcca taacgcgtcc ccttcttagc
    33961 ggttgaacta acggacacct ttcgggatgg aaaaaactta ctgacctgga cttgccctct
    34021 cgtttgttag cttaactata agccactctt tgcaggtttt catcgcatta ttacgaaaaa
    34081 ttacaattag gaaggaagcg ggacagggct cactttttca tactagcacc aacccgcagc
    34141 aggtaaaagc agtttgctcg aactgttcat aacatttcag gattatcgcc agatgaaatt
    34201 ggtgacacta ataccaggat tgctctgaat atgacgtaat aaccgaggaa tgaataaaga
    34261 attaccgcaa ataattaaga atagcctcta aatgattatg
//
LOCUS       DAZFAB010000039        34284 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZFAB010000039 DAZFAB010000000
VERSION     DAZFAB010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.039"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(109..784)
                     /locus_tag="ACHJWA_002237"
                     /pseudo
     CDS             complement(109..784)
                     /locus_tag="ACHJWA_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(929..1537)
                     /gene="yiiX"
                     /locus_tag="ACHJWA_002238"
     CDS             complement(929..1537)
                     /gene="yiiX"
                     /locus_tag="ACHJWA_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFO4308832.1"
                     /translation="MKIRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(1597..1914)
                     /gene="metJ"
                     /locus_tag="ACHJWA_002239"
     CDS             complement(1597..1914)
                     /gene="metJ"
                     /locus_tag="ACHJWA_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFO4308833.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            2191..3351
                     /gene="metB"
                     /locus_tag="ACHJWA_002240"
     CDS             2191..3351
                     /gene="metB"
                     /locus_tag="ACHJWA_002240"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFO4308834.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAENPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAKAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            3354..5786
                     /gene="metL"
                     /locus_tag="ACHJWA_002241"
     CDS             3354..5786
                     /gene="metL"
                     /locus_tag="ACHJWA_002241"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFO4308835.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPTSQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(5925..6059)
                     /locus_tag="ACHJWA_002242"
     CDS             complement(5925..6059)
                     /locus_tag="ACHJWA_002242"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4308836.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            6135..7025
                     /gene="metF"
                     /locus_tag="ACHJWA_002243"
     CDS             6135..7025
                     /gene="metF"
                     /locus_tag="ACHJWA_002243"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFO4308837.1"
                     /translation="MNFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            7354..9534
                     /gene="katG"
                     /locus_tag="ACHJWA_002244"
     CDS             7354..9534
                     /gene="katG"
                     /locus_tag="ACHJWA_002244"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFO4308838.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            9628..10533
                     /gene="yijE"
                     /locus_tag="ACHJWA_002245"
     CDS             9628..10533
                     /gene="yijE"
                     /locus_tag="ACHJWA_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFO4308839.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAAVFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKRI"
     gene            complement(10560..11177)
                     /gene="yijF"
                     /locus_tag="ACHJWA_002246"
     CDS             complement(10560..11177)
                     /gene="yijF"
                     /locus_tag="ACHJWA_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFO4308840.1"
                     /translation="MKASLALVSLLTAFTSYSLKSPAIPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYVQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(11451..12554)
                     /gene="gldA"
                     /locus_tag="ACHJWA_002247"
     CDS             complement(11451..12554)
                     /gene="gldA"
                     /locus_tag="ACHJWA_002247"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFO4308841.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENSPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(12565..13227)
                     /gene="fsa"
                     /locus_tag="ACHJWA_002248"
     CDS             complement(12565..13227)
                     /gene="fsa"
                     /locus_tag="ACHJWA_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO4308842.1"
                     /translation="MELYLDTANVAEVERLAHIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(13239..15740)
                     /gene="ptsP"
                     /locus_tag="ACHJWA_002249"
     CDS             complement(13239..15740)
                     /gene="ptsP"
                     /locus_tag="ACHJWA_002249"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO4308843.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFSRSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            16049..17128
                     /gene="frwC"
                     /locus_tag="ACHJWA_002250"
     CDS             16049..17128
                     /gene="frwC"
                     /locus_tag="ACHJWA_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFO4308844.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            17143..17463
                     /gene="frwB"
                     /locus_tag="ACHJWA_002251"
     CDS             17143..17463
                     /gene="frwB"
                     /locus_tag="ACHJWA_002251"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO4308845.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            17514..19811
                     /gene="pflD"
                     /locus_tag="ACHJWA_002252"
     CDS             17514..19811
                     /gene="pflD"
                     /locus_tag="ACHJWA_002252"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO4308846.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELGQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAASCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFDQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVGNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            19777..20655
                     /gene="pflC"
                     /locus_tag="ACHJWA_002253"
     CDS             19777..20655
                     /gene="pflC"
                     /locus_tag="ACHJWA_002253"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312908.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFO4308847.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            20657..20998
                     /gene="frwD"
                     /locus_tag="ACHJWA_002254"
     CDS             20657..20998
                     /gene="frwD"
                     /locus_tag="ACHJWA_002254"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO4308848.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCLLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQQVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(20985..21836)
                     /gene="yijO"
                     /locus_tag="ACHJWA_002255"
     CDS             complement(20985..21836)
                     /gene="yijO"
                     /locus_tag="ACHJWA_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4308849.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(22051..23784)
                     /gene="eptC"
                     /locus_tag="ACHJWA_002256"
     CDS             complement(22051..23784)
                     /gene="eptC"
                     /locus_tag="ACHJWA_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFO4308850.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNMFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELNALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKENTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(23967..26618)
                     /gene="ppc"
                     /locus_tag="ACHJWA_002257"
     CDS             complement(23967..26618)
                     /gene="ppc"
                     /locus_tag="ACHJWA_002257"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFO4308851.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKTLWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(26970..28121)
                     /gene="argE"
                     /locus_tag="ACHJWA_002258"
     CDS             complement(26970..28121)
                     /gene="argE"
                     /locus_tag="ACHJWA_002258"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFO4308852.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASTGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICAWCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            28275..29279
                     /gene="argC"
                     /locus_tag="ACHJWA_002259"
     CDS             28275..29279
                     /gene="argC"
                     /locus_tag="ACHJWA_002259"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFO4308853.1"
                     /translation="MLNTLIVGASGYAGAELVTCVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLDAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKPGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            29287..30063
                     /gene="argB"
                     /locus_tag="ACHJWA_002260"
     CDS             29287..30063
                     /gene="argB"
                     /locus_tag="ACHJWA_002260"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFO4308854.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            30124..31497
                     /gene="argH"
                     /locus_tag="ACHJWA_002261"
     CDS             30124..31497
                     /gene="argH"
                     /locus_tag="ACHJWA_002261"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFO4308855.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEELPLTELQKFSPVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FARARLG"
     gene            31764..32681
                     /gene="oxyR"
                     /locus_tag="ACHJWA_002262"
     CDS             31764..32681
                     /gene="oxyR"
                     /locus_tag="ACHJWA_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFO4308856.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(32664..34064)
                     /gene="sthA"
                     /locus_tag="ACHJWA_002263"
     CDS             complement(32664..34064)
                     /gene="sthA"
                     /locus_tag="ACHJWA_002263"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFO4308857.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
BASE COUNT         7749 a         8654 c         9565 g         8316 t
ORIGIN      
        1 aaaaaagagc agaatgaggt aaaaatcttt ggctgtgtat tcagtctggt aatcatccaa
       61 ggaaagctca tcagaagggt gatgactcca attagtagaa catatatttt cgataaaagc
      121 accgatgact ttttcatgaa gttctacaga actcgagaaa cagatggttc tgcgggccag
      181 acgtttgatt cgggttctca gggtcagatt gttacgctca atgtgctgag taaaaatctt
      241 accagtcaga tgcttctcct ttggtaactc tctagcgtaa ctaccccatt cgtcagtggt
      301 caacatgccg atggtaaaag ggtaagcagc gccagcaatt cacgacaggt ttcatcagtc
      361 cgggggccaa aagtgtaggc cagaacacca ccagttttcg tgttgtacac gtaccagagc
      421 cagtgctgtc gcgctttact gccgacaaag ctccattgct gactaagttt gcagatgagt
      481 gccacatcag catgagcgac cggggatgac gttattcttc gtggcgagag ttttttaaag
      541 ttcggatgac tgtattaatg ccgattttca gcgtacgggc agtatagcga accccggcac
      601 cgttgaaagc catttcagcg atttggtctt taactcccgg tttacggggc tcgtaacagt
      661 aactgagctg aaatacccgg tgacagtcac ggccgcggaa tcgatcatgt cttttggggt
      721 tctgcccgcg acggtaaacc tgaacggact ggcaacgggg acaatgaagg ttaactgtga
      781 ccatgaaaca tccttaaaag cgagtattat atccttaatt caactaattg gaggcatcac
      841 cgtttaccgg agcgacagct tgcggaaagg tcgtgcaact acaaagcaac aaacagacta
      901 cggcgggaat tatttttttc atcatccatt accacgaaaa cagcggccat tctttggcaa
      961 ccgtggtgag ttgtggcgca tcgaaaacgg cttgtggtga gaccaccgtt tcctcaagcg
     1021 gaatattttt gccgtagcgt tctttgagct tcgcctggac cagcgggttg ctgagatcaa
     1081 actctttcaa cttctgctgt tcgcccacac gcattcccag cgcattctga taaaccttcc
     1141 acaccacttc cgaacagtac tggcgatcgt cgctccatga gaagctgaaa tcgtacggtt
     1201 tgccgagata acgtttcgcc gtttgcgcca gtttttgctg ttgttcaaca ctcagtccgc
     1261 cttcaacgcg gcgaacaacg tatttgccct tttcaccatg ggcaatccac tgcttgagcg
     1321 gggtgtattt caccgggccg accgcttcaa aaacgtaggg ctttttgtta cgcatcacca
     1381 gcataccggt gtggctgtaa tcggaatggg tcgccagttg gatcgcttta ctttgcgatg
     1441 agcgagagat ttgaaagatg atgtcgccag tttgcggctg ccaggcaaag gcaggcacag
     1501 aaaccagcag gctgaggatc agtagcctga ttttcatcgt taaacgtcct tgtatgaaaa
     1561 aagcggctgg ttaactccgc gccgctcttt tccgctttag tattcccacg tctccgggtt
     1621 aatccccatc tcacgcatga tctcttttgc cgcttccggg atttcgtcgc tgcgctcttt
     1681 acgcagatcg gcatcatccg gcaaaggttg cccggtaaag gcatgcagaa acgcttcgca
     1741 cagcagctcg ctgttggtag cgtgacgcag gttgttcacc tgacgacgcg tgcgttcatc
     1801 ggtgaggatt tttaacacct taagaggaat ggaaaccgta atctttttga cttgttcact
     1861 cttcttgccg tgctcagcgt atgggctgat atattcgccg ctccattcag ccatgagata
     1921 cttaatcctc ttcgtcaata aattgagacc agaccacaca atttatgtgg gtactgaccg
     1981 taaacccgca tagtttaccg tacaggcgtt accgtgacat cgtgtaatgc acctgtcggc
     2041 gtgataatgc atataatttt aacggctatt tgggatttgc tcaatctata cgcaaagaag
     2101 tttagatgtc cagatgtatt gacgtccatt aacacagtgt ttactctggt gcctgacatt
     2161 tcaccgacca agcccaggga acttcatcac atgacgcgta aacaggccac catcgcagtg
     2221 cgtagcgggt taaatgacga cgaacagtat ggttgcgttg tccctcccat ccatctttcc
     2281 agcacctata actttaccgg atttaatgaa ccgcgcgcgc atgattactc gcgtcgcggc
     2341 aacccaacgc gcgatgtggt tcagcgtgcg ctggcagaac tggaaggcgg tgctggtgca
     2401 gtacttacta ataccggcat gtccgcgatt catctggtaa cgaccgtctt tttgaaacct
     2461 ggcgatctgc tggttgcgcc gcacgactgc tacggcggta gctatcgcct gttcgacagc
     2521 ctggcgaaac gcggttgcta tcgcgtgttg tttgttgatc aaggcgatga acaggcatta
     2581 cgggcagcgc tggcagaaaa ccccaaactg gtactggtag aaagcccaag taatccattg
     2641 ttacgcgtcg tggatattgc gaaaatctgc catctggcaa gggaagtcgg ggcggtgagc
     2701 gtggtggata acaccttctt aagcccggca ttacaaaatc cgctggcatt aggtgccgat
     2761 ctggtgttgc attcatgtac gaaatatctg aacggtcact ctgacgtggt ggctggcgtg
     2821 gtgattgcta aagacccgga cgttgtcact gaacttgcct ggtgggcaaa caatattggc
     2881 gtgacgggcg gcgcgtttga cagctatctg ctgctacgcg ggttgcgaac gctggtgccg
     2941 cgtatggagc tggcgcagcg caacgcgaag gcgattgtga aatatctgca aacccagccg
     3001 ttggtgaaaa aactgtatca cccgtcgctg ccggaaaatc aggggcatga aattgccgcg
     3061 cgtcagcaaa aaggctttgg cgcaatgttg agttttgaac tggatggcga tgagcagacg
     3121 ctgcgccgtt tcctgggcgg gctgtcgtta tttacgctgg cggaatcatt agggggagtg
     3181 gaaagtttaa tctctcacgc cgcaaccatg acacatgcag gcatggcacc agaagcgcgt
     3241 gctgccgccg ggatctccga gacgctgctg cgtatctcca ccggtattga agatggcgaa
     3301 gatttaattg ccgacctgga aaatggcttc cgggctgcaa acaaggggta aaaatgagtg
     3361 tgattgcgca ggcaggggcg aaaggtcgtc agctgcataa atttggtggc agtagtctgg
     3421 ctgatgtgaa gtgttatttg cgtgtcgcgg gcattatggc ggagtactct cagcctgacg
     3481 atatgatggt ggtttccgcc gctggtagca ccactaacca gttgattaac tggttgaaac
     3541 taagccagac cgatcgtctc tctgcgcatc aggttcaaca aacgctgcgt cgctatcagt
     3601 gcgatctgat tagcggtctg ctacccgccg aagaagccga tagcctcatt agcgcttttg
     3661 tcagcgatct tgagcgcctg gcggcgctgc tcgacagcgg tattaacgac gcagtgtatg
     3721 cggaagtggt gggccacggc gaagtgtggt cggcgcgcct gatgtctgcg gtgcttaatc
     3781 aacaagggct gccagcggcc tggcttgatg cccgcgagtt tttacgcgct gaacgcgctg
     3841 cgcaaccgca ggttgatgaa gggctttctt acccgttgct gcaacagcta ctggtacaac
     3901 atccgggtaa acgtctggtg gtgaccgggt ttatcagccg taacaacgcc ggtgaaacgg
     3961 tgctgctggg gcgtaacggt tccgactatt ccgcgacaca aatcggtgcg ctggcgggtg
     4021 tttctcgtgt aaccatctgg agcgacgtcg ccggggtata cagtgccgat ccgcgtaaag
     4081 tgaaagatgc ctgtctgctg ccattgctgc gtctggatga agccagcgaa ctggcgcgtc
     4141 tggcggctcc cgttcttcac gcccgtactt tacagccagt atccggtagc gaaatcgacc
     4201 tgcagctgcg ctgtagctac acgccggatc aaggctccac gcgcattgaa cgcgtgctgg
     4261 cctccggtac tggtgcgcgt attgtcacca gccacgatga tgtctgcttg attgagtttc
     4321 aggtgcccac cagccaggat ttcaaactgg cgcataaaga gatcgaccag atcctgaaac
     4381 gtgctcaggt gcgcccactg gcggttggcg tgcataacga tcgccagttg ctgcaatttt
     4441 gctacacctc agaagtggcc gacagtgcgc tgaaaatcct cgacgaagcg ggattacctg
     4501 gcgaactgcg cctgcgtcag gggctggcgc tggtggcgat ggtcggggcg ggtgtcaccc
     4561 gtaacccgct gcattgccac cgcttctggc agcaactgaa aggccagccg gtcgagttta
     4621 cctggcagtc cgatgacggc atcagcctgg tggcagtact gcgcaccggc ccgaccgaaa
     4681 gcctgattca gggcctgcat cagtccgtct tccgcgcaga aaaacgcatc ggcctggtat
     4741 tgttcggcaa aggcaatatc ggttcccgct ggctggaact gttcgcccgc gagcagagca
     4801 cgctttcggc gcgtaccggc tttgaatttg tgctggcagg cgtggtggac agccgccgca
     4861 gtctgttgag ttatgacggg ctggacgcca gccgcgcgtt agccttcttc aacgatgaag
     4921 cggttgagca ggatgaagag tcgttgttcc tgtggatgcg cgcccatccg tatgatgatt
     4981 tagtggtgct ggacgttacc gccagccagc agctggctga ccagtatctt gatttcgcca
     5041 gccacggttt ccacgtcatc agtgccaaca aactggcggg agcaagcgac agcaataaat
     5101 atcgccagat ccacgacgcc ttcgaaaaaa ccgggcgtca ctggctatac aacgccaccg
     5161 tcggtgcggg cttgccgatc aaccataccg tgcgcgatct gatcgacagc ggcgatacta
     5221 ttttgtcgat cagtgggatc ttctccggca cgctctcctg gctgttcctg caattcgacg
     5281 gtagcgtgcc gtttaccgag ctggtggatc aggcgtggca gcagggctta accgagcctg
     5341 atccgcgtga cgacctctcc ggcaaagacg taatgcgcaa gctggtgatt ctggcgcgtg
     5401 aagcgggtta caacattgaa ccggaccagg tacgtgtgga atcgctggtg cctgctcatt
     5461 gcgaaggcgg cagcatcgac catttctttg aaaatggcga tgaacttaac gagcagatgg
     5521 tgcaacggct ggaagcggcc cgcgaaatgg ggctggtgct gcgctacgtg gcgcgtttcg
     5581 atgccaacgg caaagcgcgt gtgggcgtgg aggcggtgcg tgaagaccat ccgctggcat
     5641 cactgctacc gtgcgataac gtctttgcca tcgaaagccg ctggtatcgc gacaacccgc
     5701 tggtgatccg tgggcctggc gcggggcgcg acgtcaccgc cggggcgatt cagtcggata
     5761 tcaaccggct ggcacagttg ttgtaatttc agaaatttaa taatgcccgg tactcatgtt
     5821 ttcgggttta tggtttctaa tgaaatatat tgaattatca taggattagg ccggatgaag
     5881 cgtttacgac gaatccggca agaagcaata agtacatggt tagtttatat ttgcagtccg
     5941 gtttgctttg cataccggat tttctttttc ttaccatcct gaagtttttt catcttccct
     6001 gatttttcct caccatcatt ggtcattttt cggttgacgc ccttcggctt ttccttcatc
     6061 tttacatctg gacgtctaaa cggatggatg tgcacaacac aacatataac tacaagcgat
     6121 tgatgaggta aggtatgaac ttttttcacg ccagccagcg ggatgccctg aatcagagcc
     6181 tggcagaagt ccaggggcag attaacgttt cgttcgagtt tttcccgccg cgtaccagtg
     6241 aaatggagca gaccctgtgg aactccatcg atcgccttag cagcctgaaa ccgaagtttg
     6301 tatcggtgac ctatggcgcg aactccggcg agcgcgaccg tacgcacagc attattaaag
     6361 gcattaaaga tcgcactggt ctggaagcgg caccgcatct tacttgcatt gatgcgacgc
     6421 ccgacgagtt gcgcaccatt gcacgcgact actggaataa cggtattcgt catatcgtgg
     6481 cgctgcgtgg cgatctgccg ccgggaagtg gtaagccaga aatgtatgct tctgacctgg
     6541 tgacgctatt aaaagaagtg gcagatttcg atatctccgt ggcggcgtat ccggaagttc
     6601 acccggaagc aaaaagcgcc caggcggatt tgcttaacct gaaacgcaaa gtcgatgcag
     6661 gagccaaccg cgcgattact cagttcttct ttgatgtcga aagctacctg cgttttcgtg
     6721 accgctgtgt gtcggcgggc attgatgtgg aaatcattcc gggcatattg ccggtatcta
     6781 actttaaaca ggcgaagaaa tttgccgaca tgaccaacgt gcgtattccg gcgtggatgg
     6841 cgcaaatgtt tgacgggctg gatgacgatg ccgaaacccg caagctggtg ggcgcgaata
     6901 ttgccatgga tatggtgaag attttaagcc gtgaaggagt gaaagatttc cacttctata
     6961 cgcttaaccg cgctgaaatg agttacgcga tttgccatac gctgggggtt cgacctggtt
     7021 tataaatagt gtggctttta tgaaaatcac acagtgatca caaattttaa acagagcaca
     7081 aaatgctgcc tcgaaatgag ggcgggaaaa taaggttatc agccttgttt tctccctcat
     7141 tacttgaagg atatgaagct aaaacccttt tttataaagc atttgtccga attcggacat
     7201 aatcaaaaaa gcttaattac gatcaatttg atctacatct ctttaaccaa caatatgtaa
     7261 gatctcaact atcgcatccg tggattaatt caattataac ttctctctaa cgctgtgtat
     7321 cgtaacggta acactgtaga ggggagcaca ttgatgagca cgtcagacga tatccataac
     7381 acgacagcca ctggcaaatg cccgttccat cagggcggtc acgaccagag cgcgggggcg
     7441 ggcacaacca ctcgcgactg gtggccaaat caactccgtg ttgacctgtt aaaccaacat
     7501 tctaatcgtt ctaacccgct gggtgaggac tttgactacc gcaaagaatt cagcaaatta
     7561 gattactacg gcctgaaaaa agatctgaaa gccctgctga cagaatctca gccgtggtgg
     7621 ccagccgact ggggcagcta cgccggtctg tttattcgta tggcctggca cggcgcgggg
     7681 acttaccgat caatcgatgg acgcggtggc gcgggtcgtg gtcagcaacg ttttgcaccg
     7741 ctgaactcct ggccggataa cgtaagcctc gataaagcgc gtcgcttgtt gtggccaatc
     7801 aaacagaaat atggtcagaa aatctcctgg gccgacctgt ttatcctcgc gggtaacgtg
     7861 gcgctggaaa actccggctt ccgtaccttc ggttttggtg ccggtcgtga agacgtctgg
     7921 gaaccggatc tggatgttaa ctggggtgat gaaaaagcct ggctgactca ccgtcatccg
     7981 gaagcgctgg cgaaagcacc gctgggtgca accgagatgg gtctgattta cgttaacccg
     8041 gaaggcccgg atcacagcgg cgaaccgctt tctgcggcag cagctatccg cgcgaccttc
     8101 ggcaacatgg gtatgaacga cgaagaaacc gtggcgctga ttgcgggtgg tcatacgctg
     8161 ggtaaaaccc acggtgctgg tccgacatca aatgtaggtc ctgatccaga agctgcgccg
     8221 attgaagaac aaggtttagg ttgggcgagc acttacggca gcggcgttgg cgcagatgcc
     8281 attacctctg gtctggaagt ggtctggacc cagacgccga cccagtggag caactatttc
     8341 ttcgagaacc tgttcaagta tgagtgggta cagacccgca gtccggctgg cgcaatccag
     8401 ttcgaagcgg tagacgcacc ggaaattatc ccggatccgt tcgatccgtc gaagaaacgt
     8461 aaaccgacga tgctggtgac cgacctgacg ctgcgttttg atcctgagtt cgagaagatc
     8521 tctcgtcgtt tcctcaacga tccgcaggcg ttcaacgaag cctttgcccg tgcctggttc
     8581 aaactgacgc acagggatat ggggccgaaa tctcgttaca tcgggccgga agtaccgaaa
     8641 gaagatctga tctggcaaga tccgctgccg cagccgatct acaacccgac cgagcaggac
     8701 attatcgatc tgaaattcgc gattgcggat tctggtctgt ctgttagtga gctggtatcg
     8761 gtggcctggg cttctgcttc taccttccgt ggtggcgaca aacgcggtgg tgccaacggt
     8821 gcgcgtctgg cattaatgcc gcagcgcgac tgggatgtga acgccgcagc cgttcgtgct
     8881 ctgcctgttc tggagaaaat tcagaaagag tctggtaaag cctcgctggc ggatatcatc
     8941 gtgctggcag gtgtggttgg cgttgagaaa gccgcaagcg ccgctggttt gagcattcat
     9001 gtaccgtttg cgccgggtcg cgttgatgcg cgtcaggatc agactgacat tgagatgttc
     9061 gaactgctgg agccaattgc tgacggtttc cgtaactatc gcgctcgtct ggatgtttcc
     9121 accaccgagt cactgctgat tgataaagcc cagcaactga ccctgaccgc gccggaaatg
     9181 accgcgctgg tgggcgggat gcgtgtactg ggtgccaact tcgatggcag caaaaacggc
     9241 gtcttcactg accgcgttgg cgtattgagc aatgacttct tcgtgaactt gctggatatg
     9301 cgttacgagt ggaaagcgac cgacgaatcg aaagagctgt tcgaaggccg tgaccgcgaa
     9361 accggcgaag tgaaatacac tgccagccgt gcggatctgg tatttggttc taactctgtc
     9421 ctgcgtgcgg tggctgaagt ttacgccagc agcgatgctc acgagaagtt tgttaaagac
     9481 ttcgtggcgg catgggtgaa agtgatgaac ctcgaccgtt tcgacctgct gtaatctgac
     9541 cttcttcagc gactgccttt caggcagtcg ctgaagtttc tttaccggcg tatagtgtcc
     9601 acaggaaaac tacacactgg atctctcatg tctgccgcag gaaagagcaa cccactggca
     9661 atcagtggcc tggttgtgct cacacttatc tggagttata gctggatttt catgaagcaa
     9721 gtcaccagct acatcggtgc cttcgacttt accgccttac gctgcatttt cggcgctctc
     9781 gttttattca tcgtcctttt attacgtggt cgcggaatgc gcccgacacc gtttaaatac
     9841 accttagcca ttgccctgtt acaaacctgc gggatggttg gcctggcgca gtgggcgctg
     9901 gtcagcggcg gagcggggaa ggtggcgatc ctgagctata ccatgccgtt ctgggtggtg
     9961 attttcgccg cggtgtttct cggtgaacgc ctgcgacgtg ggcaatattt cgcgattctg
    10021 attgccgctt tcggcttatt tttggtgttg cagccgtggc aactcgattt ctcttcgatg
    10081 aaaagtgcca tgctggcaat cctctccggt gttagttggg gggcgagcgc gattgttgct
    10141 aaacgtctgt atgcccgtca tccgcgcgtg gatttattgt cgttaacatc ctggcagatg
    10201 ctctatgcgg cgctggtgat gagtgtggtc gctttactgg tgccgcaacg tgaaattgac
    10261 tggcagccca ctgtgttctg ggctctggcc tacagtgcga ttctggcgac ggcgttggcg
    10321 tggagcttat ggctgtttgt gctgaaaaac ttgcctgcca gtattgccag cttaagcaca
    10381 ctggccgttc ccgtttgcgg cgtactcttt tcctggtggc tgctcggtga gaatccgggg
    10441 gccgttgaag gtagcggtat tgtgctgatt gtgctggcac tggcgctggt gagccgtaag
    10501 aaaaaagaag ccgttagtgt aaaaaggatc tgaatttttt cttcatgtgg ggcgatcgct
    10561 tatttaacaa aataacgata atgccccacc atgcgccagc taaacagcac atcttcttcc
    10621 tgcgcacctg cgccaatgtt atgtatcacc agcggcgtac cgtcgcgggc gaagccatct
    10681 gaaaccaccc caatatgtgc cagcccgtta tctaatcgcc aggagacaat atcgcccgct
    10741 tgatagtcac tggggttctt gctgatgggg cgtgttttat cgtggcgggt aaaccaggtt
    10801 tccagattag gcacccgacg gtgatcgatg ttgctgtccg ggcgctttaa ctgccatttt
    10861 tgcgggtact cggcaaaatt cttcgccata tcttcgtgca ccagtttctg caaatcgacc
    10921 ttctggctgc gcaatgcacg gatcaccaca tcggaacata ccccgcgttc ttgcgggaca
    10981 tcaccgccag gataggtaag ctgcacatac gccggatcgt aaaatagcgt gctgccgatt
    11041 tgctgtctgg caccgtctgc gatggcaagg ttggtattgg cctggatttg taccacggtt
    11101 ggtggaatgg cgggagattt taaggagtag ctggtaaatg ccgttagcag gctgaccagc
    11161 gccagtgaag ctttcatctg tttcgtccgt gaaaataaga gtcacagatt cgaccttccg
    11221 ggctaacgat tgttctgtag agaaaatgtc cagaagcgga taaaaaattc ctgaacaacg
    11281 tgggagtttc agattactgg cacagagtgc cggatgcggc gtgaacgtct tatctggcct
    11341 aaaaagcacg caaattcaat atattgcaga gattgcgtag gcctgataag cgtagcgcat
    11401 caggcaattt tgcgttcaaa ctcccggaca agccgggagt ttggagtagg ttattcccac
    11461 tcttgcagga aacgctgacc gtactggtcg gctaccagca gagcggcgta aacctgatct
    11521 ggcgtcgcgc cgccaggcat gttgtggatg gtttcacctt ctgcacatgc cgcttctgcc
    11581 acaattcgca ttttcgccgg gacatcttct ttaatatcca gttgagcgag agttattggc
    11641 aaacctaccg catggctaag cgcagctacg gtttcgattt cctcaaccgg cgagttttcc
    11701 agtaccagct gcgtcagtgt accaaatgcc actttttcac cgtgataata gtgatgggca
    11761 tcaggaatgg cagtcaggcc attatgcacc gcgtgcgctg ccgccagacc accgctttca
    11821 aagccaacac cgcttaaata agtgttcgct tcaatcacgc gttccagtgc cggagtcact
    11881 acatgctgtt cggcggcgag catcgctttt tcgccttctt ccagcaaggt gttgtagcac
    11941 agttcagcca gcgccagcgc cgcctgagtg cacttgccgc ccgccatggt ggtcgcaccg
    12001 ctacgagagc aggcacgcgc ttcaaaccag gtcgccagcg catcgccgat acccgccgct
    12061 aacagacgcg caggtgcgcc cgcgacgatt ttagtatcga caatcaccat attcgggtta
    12121 tttggcagca gcagatagcg gtcaaattca ccttcgtcag tgtagataac ggaaagagcg
    12181 ctacaaggtg cgtcggttga agcaatggtc ggggcaatgg cgaccggaac acccatgaaa
    12241 tgtgccagtg ctttggctgt atcgagggtt tttccgccac cgataccgag aattgcgcca
    12301 cactgcgcag tctccgcgat gccacgcaga cggtcgatct cattttgcga acattcaccg
    12361 ccaaacggcg caatctctac taccagtcca gcatctttaa agcttttctc gacagtggat
    12421 tgagcaaaac ctaaaacaaa tttgtcaccc accactaacc agcgttctgc cagcggcttc
    12481 aggtattcgc ccagacgatt aatcacatca gcgccctgga tgtatttacc cggtgattga
    12541 ataatgcggt ccataattgc tcctttagag atgagtagtg ccaaatgcgg cattccagtc
    12601 gtgttcgaac ttctctatag ctgactctac cgcaggggtg ttgagcattt gttgcgctac
    12661 atctaagggc agggtgatgg attcacatcc tgccagtaaa cagtccagcg cctgacgcgg
    12721 cgttttaaag ctggccgcca gcaccatgct ttctggtgcg tgcatttcta acagcgcttg
    12781 cagctcctga accgtacgaa tgccgtcccc gccctgggca tccacgcggt taacatacgg
    12841 agcaacgtat tttgcccctg ccagtgcggc taataaccct tgtgcggcgc tatatacagc
    12901 agtgccaagt gtagtaatac cctctttttt cagtatttta attgctgcca gaccttcgga
    12961 agtcaccggg attttcacca caatgcccgg aatggcatcg cgcagacgct tcgcttcttc
    13021 caccatcccc tgcgcgtcgc ggctcatggt ctgagcaaac agaatgccct catcaccaat
    13081 cgctttttgc agacgcggca gcacttccca tatggactcc ttgctggcag cgataatgct
    13141 cgggttagtt gtcaccccgg caatcgggaa tatgtgtgcc agacgttcga cttctgcgac
    13201 gttagcggtg tccagataca gttccatgat gtttcctttt acagttccag ttcatgttgc
    13261 agcaggctgg cgatagcgtc ggcagaggcg gcgttaacca gcgcgttacg gaattcttcg
    13321 tgcatgatgc ggcgagcgag gcgcgagaaa atgcgcatat gctgatcgcc cgcagcgtgt
    13381 ttgttcaggg ttaacataat gatgaattgc gcttcatcat cgccccagcg caccggcgct
    13441 tgcagacgcg ctacgctgat ggtggattgc tcaatgtgtt ctgatttgct gtggggaatg
    13501 gcaaaactaa agcccagacc ggtagagaaa acggcctcgc gcgcccacaa gtcggcttcc
    13561 agtttacgcg gatagcggca gcggcccgcc agcagcaggt tatcggtcat gcctttgagc
    13621 acttcttctt tgctgcgcca gtcgctttcc agtgtgatgc actcggcggt gaccagcggt
    13681 gcgtcttgtt gggtcatgcg gaattgcgcc agcaggtgtt ccacttccag cgaagtacgg
    13741 caggccattg cctggttgag caacttgcgg cactcacggc tatcaagttg cgccatccgc
    13801 gctttcgccg ccggaattga tggtgcgctc atgctgagtt catccagccc taagccgacc
    13861 agcaacggca gcacggaacc tttcgctccc agctcaccgc acagaccaat ccatttgccc
    13921 tggcgatgca ccgcttgcac ggcgtaatcg agcgcccgca agaatgccgg attcaggctg
    13981 ttgtagtgac gagtaacctt agcgttatcg cgatccactg ccagcagata ttgcgtcagg
    14041 tcattactac caatactaaa gaaatcaatc tcttcgcagc attgatcgat gatgaacatc
    14101 accgacggca cttccagcat gatgccgagc tggatcttct catcaaacgg aatgtgttcg
    14161 ttacgcagtt gctgtttggc ttccgccagt ttttctttca cccataagat ctcttccatt
    14221 gaggagatca tcgggatcat gattttcagg ctgccgtgag cagaggcacg gaggatcgac
    14281 cgtagttgtg tggtgaacag cgaggcgtat tcttcataaa tacgcaccgc gcgataaccg
    14341 aggaacgggt tagcttctgc cggaatgttc agataatcaa cgggtttgtc accgccaata
    14401 tccatagtgc gcacaataat gctgcgtccg ttggcggact ccagcgcttg acaaaaaatg
    14461 ttgtacaact cgctttcgcc cggtgcgctg gtgcgatcca tatagagcat ttcagtgcgg
    14521 aacaaaccga cgccttccgc accattgccg aatgcggcct gcgcttccac ggagtgagcg
    14581 atgttagcgg caatttcaat gcggataccg tcagcggtac gggcttgttg ggtcagccag
    14641 acacgctgtt gctcacgcag ggcgtcctgt acgcgggctt cttgctgata ataacgagct
    14701 acggcttccc ctggctcaac cacaatcgcc ccggcgttgc cgtcgatata aatcgtttga
    14761 tgctgccacg gagtaagggc atcaatatct acaccaacca gtgtcggaat gttgaacgaa
    14821 cgggcaagaa ttacggtatg tgaggtggtg ccgccacttt tcagtaacag acctttgagg
    14881 tgatttttat ccagttcgag gaactggctg ggggttagtt catcagccat acaaatggcg
    14941 ggctgcgtca gtttgcccgg tgccgggaag cgttgctcac cgtagatttg ctgaagcagc
    15001 tggaagcaga catcgcgtac gtccagggca cgttcttgca ggtagctgct gctggaacgg
    15061 gaaaactctt cacaaaagtg attcgcactg gcgacaatcg cttcggcgca gcttaatccg
    15121 gcgctgacgc ctgccagtaa atgttcgtgc agggaagtat cgccagccag ggatcggtgc
    15181 gcttccagaa tcgcgctggt ggcaccgtcg ctatccagca gacgaaactc aatgtttttc
    15241 agtaccagcg tcaggccgtt ttccagtgcg gattgctcgg cgtcaacgtc tttggctgcg
    15301 ggaagattac ccagcgcatt gagatctaaa gaagagatcg gcgtcagaat gccgcctgcg
    15361 ctaccgctgc acacggtgcg ggcgcggata atttgcggat tcagattggt cagtgaaacc
    15421 ggcagtggtt ccagttcgtc agatttaact tccgccagcg gcgcatcgca gtgggggaat
    15481 tcatcgcgca gccattggct taaacgctgg tgcgcttcct gttcgtcggc cccggaaatc
    15541 agtaactggc agttatcgcc cgccagcgtg tcggtgccaa tcagcgcaag ggcgcttttg
    15601 gcgttgccct tgcggtcagt gcgcaggtta tgccactcaa tttgtgatga aaaagtatta
    15661 cacagcgttt caacgtggct tgccggacgt gcatgtacgc cgttaggtag ctcacaaata
    15721 aattccacaa tcagggccat tgcctctctc ccggtacggt tttctgttta caggataaga
    15781 gcgcacggca acggcctgcc atgtgacaaa tctgccaaaa gctggacaaa tgtaatgtaa
    15841 ccgtcaattt gcgacgcgtc tcacaagacg ccgttttgcg gcatgcttcc ggattatcgc
    15901 aagttatgag gcggatcgca tttttgtact gatattacaa aagtccagta aatggccttt
    15961 ttatccactg ttttctccgc ctgcgattgc ctattgtttc ctccatcaaa aatatggaca
    16021 tgggctttcg caagcccggg agcagcttat gaatgagttg gtgcagatcc tgaaaaatac
    16081 ccgtcagcat ttaatgacgg gcgtttcaca catgattccc ttcgtggtat cgggcggtat
    16141 tttgctggcg gtttccgtca tgttgtatgg caaaggcgca gtgccggatg ccgtagccga
    16201 tccgaatctg aaaaaactgt ttgatatcgg cgttgcaggt ttgacgctga tggtgccttt
    16261 cctcgccgct tacatcggtt actccattgc agagcgttct gcgctggctc cgtgcgctat
    16321 cggtgcctgg gttggtaaca gctttggtgc gggcttcttt ggtgcactga tcgccgggat
    16381 tatcggcggc atcgtggtgc attacctgaa gaaaattccg gtgcataaag ttctgcgctc
    16441 ggtgatgccc atcttcatca ttccgatcgt cggcacactg attaccgcag gcatcatgat
    16501 gtggggtctg ggcgagcctg taggggcgtt gaccaacagc ctgactcagt ggcttcaggg
    16561 gatgcagcag ggcagcattg ttatgctggc ggtgatcatg ggtctgatgc tggcgttcga
    16621 tatgggcggt ccggttaaca aagtggccta tgccttcatg ctgatttgcg ttgctcaggg
    16681 tgtttatacc gtggtggcca ttgctgccgt gggtatttgt atcccgccgc tggggatggg
    16741 gctggcgacg ctgattggtc gtaaaaattt ctccgcagaa gagcgcgaaa ccggtaaagc
    16801 ggcgctggtg atggggtgcg ttggggttac tgaaggggcg attcctttcg ccgcagccga
    16861 tccgttacgt gtaattcctt ccatcatggt tggttcagtt tgtggtgcag taactgcggc
    16921 gctggtcggt gcgcagtgct atgcaggctg gggtggtctg attgtgctgc cggtggttga
    16981 aggtaagttg ggttatatcg cagcagtggc tgtcggggcg gttgtgacgg cagtctgcgt
    17041 gaacgtgctg aaaagtctgg cgcgtaaaaa tggatcttct actgatgaaa aagaagacga
    17101 cctggatttg gattttgaaa ttaattaatt gaggaacgag ccatgacgaa aattattgca
    17161 gtaaccgcgt gtccatctgg tgttgcccat acttatatgg ctgcagaggc gctggaaagt
    17221 gccgcgaaag cgaaaggctg ggaagtgaaa gtagaaaccc agggctcgat tggtctggaa
    17281 aacgaactga ctgcggaaga cgtggcgagc gccgatatgg tgattctgac taaagatatc
    17341 ggcatcaagt ttgaagagcg ttttgctggc aagaccatcg tgcgcgtcaa catcagcgat
    17401 gcggtaaaac gtgctgatgc cattatgagc aaaattgaag ctcacctggc gcaaaccgct
    17461 taagttcttc tcccgctcgc aagggcgggt tcgctttccc acaggagttc ctcatgacga
    17521 atcgtatctc tcgcctcaaa actgcactgt ttgccaatac ccgtgaaatc tcgctggagc
    17581 gtgcgctgct ttataccgcc agccatcggc aaaccgaagg cgaaccggtg atattgcgcc
    17641 gggcgaaagc aacagcgtat atccttgaac atgttgaaat ttcgattcgt gatgaagaac
    17701 tgattgccgg taaccgcacc gtaaaaccgc gcgccgggat tatgtcgccg gaaatggacc
    17761 cttactggct gctgaaagag ctgggtcaat tcccgacgcg tccgcaggac cgttttgcta
    17821 tcagcgaaga agataaacgt atctaccgcg aagagttgtt cccgtactgg gaaaaacgtt
    17881 cgatgaaaga tttcatcaac gggcagatga cggatgaagt aaaagccgcg actagcacgc
    17941 agattttcag catcaaccag acggataaag gccaggggca cattattatt gattacccgc
    18001 gcctgctgaa tcacgggctg ggggagctgg tggcacagat gcagcaacat tgtcagcaac
    18061 agccggagaa tcacttttat caggcagcgc tgttactgct cgaagcctcg cagaaacaca
    18121 ttttgcgtta cgccgaactg gcggaaacga tggcggcaag ctgcactgat gcccagcgtc
    18181 gcgaagaact gttgactatt gcggagatct cccgccataa cgccgaacat aaaccgcaga
    18241 cgttctggca ggcgtgccag ttattctggt acatgaacat cattctgcaa tacgaatcca
    18301 acgccagttc gctgtcttta gggcgcttcg accagtatat gttgccgttc tatcaggcat
    18361 ctttaaccca gggcgaagat ccggcgttcc tgaaagaact gctcgaatct ttatgggtga
    18421 aatgcaacga cattgtgctg ttgcgctcca ccagcagcgc gcgttatttc gcaggtttcc
    18481 cgactggcta taccgcactg ctcggcgggt taaccgagaa cggacgtagc gcggtgaacg
    18541 tgctttcgtt cctctgcctc gatgcctatc aaagcgtaca attaccgcaa ccgaacctag
    18601 gggtgcgcac taacgccttg atcgacacgc cgttcctgat gaaaaccgca gaaaccattc
    18661 gcctcggcac cggtattccg caaatcttta acgatgaagt ggtggtgcca gcgttcctca
    18721 accgtggcgt ttcgctggaa gatgcgcgcg actattccgt agtgggctgt gtggaattat
    18781 ctattcccgg cagaacctac ggcttgcatg acatcgcgat gtttaatctg ctgaaagtga
    18841 tggaaatctg cctgcatgaa aatgaaggca atgctgcgct gacttatgaa ggtttactgg
    18901 agcagatccg cgccaagatc agccactaca tcaccctgat ggttgagggc agcaatattt
    18961 gtgatatcgg ccatcgcgac tgggcacctg taccgctgct ctcgtctttt atcagcgatt
    19021 gtctggaaaa aggtcgcgat attaccgatg gcggcgcgcg ttataacttc tccggcgtac
    19081 agggaattgg tatcgccaac ctgagcgatt ctctccatgc gttgaaaggg atggtttttg
    19141 atcaacagcg tttaagtttt gacgaattgc tgtcggtatt aaaagccaac tttgcaacgc
    19201 cagaaggcga aaaagtccgc gctcgcttaa ttaaccgctt tgagaaatac ggtaacgata
    19261 tcgacgaggt gggtaacatt agcgccgaac tgttgcgcca ctactgcaaa gaagtggaaa
    19321 aataccagaa cccgcgcggc ggttacttca cgccgggatc gtataccgtt tctgctcacg
    19381 tcccgttggg atcggtggtt ggcgcgacgc cagacggtcg ttttgccgga gaacagttgg
    19441 cagacggcgg cttgtcaccc atgctgggcc aggacgcaca agggccaaca gcggtactga
    19501 agtcagtcag taagctcgat aacacgctgc tgtctaacgg tacgttgctg aacgtgaaat
    19561 tcactccggc gaccctggaa ggtgaagcgg gattacgcaa actggccgac ttcttacggg
    19621 cgtttaccca gcttaagttg cagcatattc agtttaacgt ggtgaacgcc gacacgttgc
    19681 gggaagcgca acagcgccca caagattatg ccgggctggt ggtgcgcgtt gccggataca
    19741 gcgccttctt tgtcgaactg tcgaaggaga tccaggatga catcatccgc cggacagcgc
    19801 atcagctgta acgttgtgga aacgcgccgc aatgatgtgg cgcgcatttt caacattcag
    19861 cgttattcac tgaatgacgg cgagggcatt cgtacggtgg tcttttttaa aggctgtccg
    19921 catctttgcc cgtggtgtgc taatccggag tcgatctccg gcaaaatcca gacggtacgc
    19981 agagaggcga aatgtctgca ctgtgcgaaa tgtttgcgtg atgcggatga atgcccctcc
    20041 ggggcgtttg aacggattgg tcgcgatatc agccttgacg cgctggaacg ggaagtgatg
    20101 aaagatgaca ttttctttcg cacgtccggc ggcggcgtca cgctttctgg cggcgaagtg
    20161 ttaatgcagg cggagtttgc tacccgtttt ttacagcggc tgcggttgtg gggcgtgtca
    20221 tgcgccattg aaactgccgg agacgcgcca gccagcaagc tgttaccgct ggcgaaattg
    20281 tgcgatgaag tgttgttcga tttaaaaatt atggacgcga ctcaggcgcg ggatgtggtg
    20341 aagatgaacc tgccacgcgt gctggagaat ctgcgtttgc tggtgagtga gggcgtcaac
    20401 gtgatcccgc gtttaccgct gatccctggt ttcacgctca gccgggagaa tatgcagcag
    20461 gcgctggatg tactgatccc gctgaaaatc aggcagatcc atctgttacc gtttcatcag
    20521 tacggcgaac cgaaataccg cctgctgggg aaaacatggt cgatgaaaga ggtgcctgcg
    20581 ccgtcgtcag ccgatgtgac aacgatgcgc gaaatggcag aacgggccgg atttcaggtt
    20641 accgtgggag gttaaaatgg catacctggt ggcagtaacc gcctgcgtaa gcggcgtggc
    20701 gcatacatat atggcggcgg aacggctgga aaagttgtgc ctgttagaga agtggggagt
    20761 cagcattgaa actcagggcg cgctgggaac ggagaatcgt ttagcggacg aggatatccg
    20821 tcgggcggat gttgctctgt tgattacgga tatcgagctt gccggtgccg agcgatttga
    20881 acattgccgc tatgtgcaat gcagcatcta cgcattcctg cgtgagccgc agcaggtaat
    20941 gagcgcggtg cgcaaagtgc tttctgcgcc gcagcaaacc catcttattc tggagtagtc
    21001 ggtttttctg tcagttggct gtggtactgc cggcgatatt ccgacggcga gcgttcggtg
    21061 tttttacgaa acagacggca gaagtagttg ctgtcgacaa agccgcaggc gtgcgccact
    21121 tcttttacct tcaggtcata gcctttcaat aacgtcttag cgtgctccag tcgcgtgtga
    21181 ttcaggtatt cgttaaaacc aatggccccc gttttttgaa acaggtgcga gagatagttt
    21241 ggtgaaatat agaatgcctg tgcgacagat tcgcgggtaa gcgcggaggc atagcgttcg
    21301 tcgatataat cgcgaatagc ttcgaatagt gcctggctgc gtgaggcggt ctggatttgg
    21361 ctgccaagca aatcgcggca gtggctaagc aggctggcga cgataagcct tgccgtttgc
    21421 tgctcctgcg gctgcatttg catttcattg agcgtttgta gcagaaaaga acctatgcgt
    21481 gggccgcgtc gggcgacgtg ttgcttcgcc agattttgat attgtttgcc atcccattgc
    21541 acgacgctga agccgagttg ctgtttgcca aacagcacgc taaaggtagt agcaggggct
    21601 tgccattgtg gaaaattcca gccaccagcg gcaacgtaca gcacatcgcc gggaactaac
    21661 gttgctccag cgccggtatc aacaaactca ccttccagca caatttccag ccgtggaaaa
    21721 tcgacctgat acgcaagatc gggcaccggg ccgttactgc tggcaaagta aatctggcgc
    21781 agggacagcg ggccgttgat caggcgggag agcagatagc tgacgtcgtg atacatgtta
    21841 ttcccttagg tatggagacg ggaaaacagt aactggcatt attaaaaaag gccacctgaa
    21901 aagtgtggcc tgaggggagt tcgatgtaaa cattgcggca gttgtattgc cggatgtggc
    21961 gtaaacgcct tatccagtct accgccgcga tctgtaggcc ggataagacg cgtcaagcgt
    22021 cgcatccggc aaatagcgcc tggctgataa ttactgatta cctacctggt cgccatacgg
    22081 cagcgtgtcg taatcgatca gcgcgttttt cttgtacggg ttaccaatcc agcgggtatt
    22141 ttctttgaac tgcggattca ccactgaacg ggttggatcg taaccgtcgt aagataagcc
    22201 cgctaaatct gaccaggtgt ggatcagttc cgcaaggctg tatttacgat caacatcctg
    22261 cgagaaatca cgcggatgag tcgcttgcca tttttccgac gtccacagca ggaacggaat
    22321 ggtgtacata tgacgcgtcg ggttgtcctc attacgcccc tgggttttat gaggcggcgt
    22381 gtcgtaaacc tcttcaccgt ggtcagagaa gtaaaccagg aaaccgttcg ggtctgctgc
    22441 tttaaagtct ttaatcaggc tggcaaccac atgatcgtta tacaggttag cgttgtcata
    22501 atcgttatat gactccagct cttccgcgtt taatcccggc ggaacatgat cggtattgcc
    22561 atcaaacttg ccctgatttt ccgggtagcg gtatttgtat ttgatatgcg tacccagcag
    22621 atgaacgatg atcagtttct tcggcgcagg gtccttcagc acttcctgga acggcttcag
    22681 cacgttagtg tcgtattcac gcgcactctg cgtacgttgc tggttcatgt agtactgctt
    22741 gtcggtctgg cgcgaaaata ccgtcagcat ggtattgcgg gcggtcatcg tctgctggtt
    22801 ggtgatccag aaggttttat aacccgcctg tttcatcatg ttcatcagcg acggctgcgt
    22861 cagatacaga tccgggttct tttcattggc aaaggtcagc gcctgttgca ggatttcaat
    22921 ggtgtacgga cgagacgtaa ctacgttatt aaacacggtc agattcggat cggttttatg
    22981 cagcgcattc agctccggcg tggtttcacg cggataaccg tacagactca tgcgtccacg
    23041 ctgggttgac tcgccaatca ccagcactaa ggtgcgcggt tcgttacccg attcatcttt
    23101 gaaattagcc agcggcggca aggcattatt ttcattcagt agctttgtta gcgagtttag
    23161 ttgctgacga tactgataat agccggtcag gaattgccac ggtgcggcag gctccatacg
    23221 cgaggccagg ttatccaacg ttttctcaaa cggcttgttt ttgataaaca tattcatggc
    23281 gatcggatgc agaatcaagc cataaagcag ggcaaaagag acaacataac gccacggctt
    23341 tggaatatag accgggcgca ggcgtgtcca cagcagaact gccaccgccg tataggccag
    23401 cgcgataagc acaattttca ggctgaaata ctggcttaaa tactcgctgg cttcgttggt
    23461 gttggtttcg aacatcacaa acagaacgct ctgcgagaac tcctgaccgt agatgacgta
    23521 gtagcacagc gccgccagag aggccgccca tagcaccacg ccgatcactg cggcaataat
    23581 tttgatccgc ttcggaaaga ggaataccgg aattaaccac agcgagctga ataacagcga
    23641 gtcgcgaatg ccgttggtgc cactataacc actgatgtaa ataatggcct gtagcagagt
    23701 agagaaaaac caaaagtaga gcaatgccca acccagggct ttccagctaa aaagaggttt
    23761 agcctggact tctgtggaat gcatagtaag aacctgtctt gaaaaaaata tcgccgaatt
    23821 taccgacaat tccttaagga tatctgaagg gatatccaga atttgaataa aatgcagaca
    23881 gaaatatatt gaaaacgagg gtgttagaac agaagtattt cagaaaaccc tcgcgcaaaa
    23941 gcacgagggt ttgcaggaga ggaagattag ccggtattac gcatacctgc cgcaatcccg
    24001 gcaatagtga ccattaacgc ctgctcgacg cgaggatccg gttcctggcc ttctttttct
    24061 gcctggcggg agcggtgcag caactcggct tgcaatacgt tcagcgggtc ggtgtaaata
    24121 ttccgtagct gaatagactc tgcaatccac ggcagatcgg ccatcagatg ggaatcgttg
    24181 gcaatcgcca gcaccacttt gatgtcttct tcttgcagat tgcgtaactc tttacctaac
    24241 ggccacagtg ttttgtctac caggcgttgg tcatagtatt ccgccagcca caggtctgct
    24301 ttggcgaaga ccatctccag catgccgaga cgcgtcgaga agaatggcca atcgcggcac
    24361 atagcctcca gctcgctctg tttgccatct tcgaccactt tttgcagcgc cgtacctgca
    24421 cccagccagg cggggagcat cagacggttt tgcgtccagg cgaagatcca cggaatggcg
    24481 cgtagtgact cgacgccgcc ggttgggcga cgtttcgccg gacgtgaacc caacggcagt
    24541 ttgcccagtt cctgttccgg cgtagcggag cggaagtaag gcacaaaatc tttgttttca
    24601 cgtacgtagc cacggtagag atcgcaggag atgactgata gttcatccat aatgcgacgc
    24661 cagctctctt tcggctcagg tggtggcagc aggttggctt ccagtatcgc cccggtgtaa
    24721 agcgacaggc tgctgacggt gatttctggc agaccatatt taaagcggat catctcgccc
    24781 tgttcagtca cgcgcaggcc acctttcagg cttcctggcg gttgtgacag cagcgccgca
    24841 tgagcaggtg cgccgccgcg accaatggaa ccgccgcgac cgtggaacaa cgtcagctca
    24901 atacccgctt tttcgcaggt tttgattaat gcatcctgtg cctgatattg cgcccaggac
    24961 gctgccatca cgcccgcatc ttttgctgag tcggaatagc caatcatcac catctgtttg
    25021 ccctgaatca ggccgcgata ccagtcgata ttcagcaact gagtcatgac atcgttggcg
    25081 ttgttcaggt catcgagagt ttcaaacagc ggagcaaccg gcatcgcaaa cccgataccc
    25141 gcttctttca gcagcaggtg gacagccagc acgtcggacg gcgttttcgc catcgagatc
    25201 acgtaggcgg caatggagcc ttgcggtgct tcggcaatca cctggcaggt atcgagcact
    25261 tcgcgcgttt cggcgcttgg ttgccagttg cgcggcagaa gcggacgttt ggagttcagt
    25321 tcgcggatca ggaacgcctg tttgtcggcc tctgaccagc tttcgtagtc gccgataccg
    25381 aggtagcggg tcagctcgcc cagcgcttcg gtatgacgcg tgctctcctg acggatatca
    25441 atacggacca gcggtacgcc gaaacatttc acgcggcgca gggtgtcgag cagatcgccg
    25501 ttggcgataa tacccatgcc acacgcctga agtgactggt agcaagcgta gagcggttcc
    25561 cacagttctt cgttttgtgt cagcaggcct tctggttttg gcagttcttc gcctttcagg
    25621 cgcgcttcca gccatgcctg tgtcgccatc aggcgagaac gcaggttttt catcagatag
    25681 cgatacggtt ctgcggcacc ttcttcgcca accagcgcca gcagttcagg ggtcgcttca
    25741 accatcgaca gttcagaaac cagaacctga atatctttca ggaacagatc ggtggctttc
    25801 cagcggctga gcagcaggac gtggcgggtg atatcggcag tgacgttcgg gttgccgtcg
    25861 cggtcaccgc ccatccacga ggtaaaacgt accggaacaa attcgacggg cagtttgtag
    25921 ccgaggttct cttccagttg ttcgttcagt tcgcgcaggt aattcggtac gccttgccac
    25981 aggctgtttt ctactacggc aaagccccat ttggcttcat ctaccgggct tggacgcagc
    26041 ttacggattt catcggtatg ccatgactgg gcgatcaact ggcgcaggcg acgcatcagc
    26101 tggttgtgtt cgtagtcagc gatatctttg ttatcgagct gttttaagca ggcgttcact
    26161 tccaccattt tgtggatcag tgtacgacgg gtaatttcgg ttgggtgagc cgtgaggacc
    26221 agttccagcg acagcgattc cactgctttt ttgatggtgt cttcgctcag ttccggctgg
    26281 tttttcagtt tacgcagggt gcgggcgatc acttccgggt tgctggcagc ttcgcctttc
    26341 ggcgaaatgc tgtggtattg ctcggcggtg ttggccaggt tcaggaactg actaaacgca
    26401 cgcgcaacgg gcagcagctc gtcgttcgac aaattttgta aggtggtgag taactcctgg
    26461 cggttagcat cattgccagc gcgtgaagat ttggacaact tacggatagt ttctacgcgt
    26521 tcaagaatgt gttctcccaa cgcatccttg atggtttctc ccagcacttt gccgagcata
    26581 ctgacattac tacgcaatgc ggaatattgt tcgttcatat taccccagac accccatctt
    26641 atcgtttgat agccctgtat ccttcacgtc gcattggcgc gaatatgctc gggctttgct
    26701 tttcgtcgtc ttttataaag ccacgtaaaa gcggtgacgt caaatgctgc gaaatcgctt
    26761 cagcaaacga ataaatagca ggaatttacg tcattaaatt cacgacgctt taaataagcg
    26821 taacttatgg gaatgttaaa aaatcgcctc aagtaacacc aaaggtgtag gtcggataag
    26881 atgtgcaagc atcgcatccg acattattgc ggcactggag tttggcaaca gtgccggatg
    26941 cggcgcaagc gccttattcg gcctacgttt taatgccagc aaaaatggtg aatgacctgg
    27001 gttatcagtt cgcgggtggg cttgataaac cgtgtttcca gatattcatc aggctgatga
    27061 gcctgattaa ttgagccagg ccccagaacc agcgtcgggc ataacgtttg aataaacggc
    27121 gcttcggtac agtaattcac cacttcggtt tttgctccga gcaatttctc aaccacttca
    27181 accagttgat gattcggtgg gcattcatag ccagggatcg gcggatgcag ctcgtcgacc
    27241 gtcagacgac ccggccagcg ttcgctcacc ggagccaatg catcgttgag caaaccatta
    27301 agttcattga gtgtcatgcc aggcagcgga cgaatatcca tatgcaactc acaccaagcg
    27361 caaatacggt tagaagcgtc gccaccgtga atatgcccga ggttgagcgt agggtatggc
    27421 acggtaaacg cttcgtagtg ataacgttct ttcaggttat cgcgcaattg caaaatatgc
    27481 ccgatggcgt cgtgcattag ttctatagcg ttaactccgc gtgctggatc gctggagtgc
    27541 cccgactggc cctgaatacg gatggcgtta gagatatgac ctttatgtgc gcgtaccggt
    27601 tgtagtgacg tcggttcgcc aatgatggcg caatccgggc gcagggcggt agtttcggca
    27661 aaataacgcg ctccggccat actggtttct tcatcagcag tcgccagaat gtagagcggt
    27721 tttttcagtt tcgtgacgtc gacatcgcgt agcgcatcaa ggataaacgc aaaaaagcct
    27781 ttcatgtcgg cggtgcctaa gccgtaaagc ttgccgtcat gctccgtcag tgtaaacgga
    27841 tcgcgcgtcc agcgaccgtc atcaaatggc accgtatcgg tatgccccgc cagcaacaag
    27901 ccgccagccc cctgtccggt acttgccagc atattaaatt tgttgcgagt tcctggtaca
    27961 ggctgcactt ccacattgaa gcctaaatct ttaaaccagt ccgccagcag agtgattaaa
    28021 tctgcattgc tttgatcgag tgcctcttcc gtggcgctta ttgaaggtgt ggcaatcaga
    28081 gcacggtaaa tctcgataaa tggcggtaat ttgtttttca ttgttgacac acctctggtc
    28141 atgatagtat caatattcat gcagtattta tgaataaaaa tacactaacg ttgagcgtaa
    28201 taaaacccac cagccgtaag gtgaatgttt tacgtttaac ctggcaacca gacataagaa
    28261 ggtgaatagc cccgatgttg aatacgctga ttgtgggtgc cagcggctac gctggcgcag
    28321 agctagtgac ctgtgtaaat cgccatccgc atatgaacat aaccgctttg actgtttcag
    28381 cgcaaagcaa tgatgcggga aagttaatct ccgatttgca tccgcagcta aaaggcatcg
    28441 ttgatctgcc gttgcagccg atgtcggata tcagcgagtt tagccctggg gtggacgtgg
    28501 tgtttctcgc caccgcccat gaagttagcc acgatttagc gccgcagttt cttgacgcgg
    28561 gctgcgtggt gttcgacctt tccggcgcgt ttcgtgttaa cgacgccacc ttctatgaaa
    28621 aatattacgg ctttacccat caatacccgg aactgctgga acaggcagcc tacggtctgg
    28681 cggagtggtg cggtaataaa ttaaaagaag cgaatttgat tgcggtgcca ggctgttatc
    28741 cgacggcagc acagttggcg ctgaaaccgt tgattgatgc cgatcttctt gacctcaatc
    28801 agtggccggt gatcaacgcc accagcggcg tgagcggtgc aggacgtaag gcagcgattt
    28861 caaatagttt ttgtgaagtt agcctgcaac cgtatggcgt ctttacccat cgccatcaac
    28921 cagagatcgc cacacacctc ggtgctgacg ttatcttcac cccgcatctg ggcaatttcc
    28981 cgcgcggcat tctcgaaacc attacctgcc gcctgaaacc gggtgtgacc caggcgcaag
    29041 tggcgcaagc gttacagcag gcgtatgccc ataaaccgct ggtgcggctg tatgacaaag
    29101 gcgttccggc gctgaaaaat gtcgttgggc taccattttg cgatatcggg tttgccgttc
    29161 agggcgaaca tctgattatt gtggcgaccg aagacaactt actgaaaggc gcggcggcac
    29221 aagcggtaca gtgcgccaat attcgtttcg gctatgcgga aacgcagtct cttatttaag
    29281 ggtgcaatga tgaatccatt aattatcaaa ctgggtggcg tactgctgga tagtgaagag
    29341 gcgctggaac gtctgtttag cgcactggtg aattatcgtg agtcacatca gcgtccgctg
    29401 gtgattgtgc acggcggcgg ttgcgtggtg gatgagctga tgaaagggct gaatctgccg
    29461 gtgaaaaaga aaaatggcct gcgggtgacg cctgctgatc agattgacat tatcaccgga
    29521 gcactggcgg gaacggcaaa taaaaccctg ttggcatggg cgaagaaaca tcagattgcg
    29581 gccgtaggtt tgtttctcgg tgacggcgac agcgtcaaag tgacccagct tgatgaagag
    29641 ttaggtcatg ttggactggc gcagccaggt tcgcctaagc ttatcaactc cttgctggag
    29701 aacggttatc tgccggtggt cagctccatt ggcgtaacag acgaagggca actgatgaac
    29761 gtcaatgccg accaggcggc aacggcgctg gcggcaacgc tgggcgcgga tctgattttg
    29821 ctctccgacg tcagtggcat tctcgacggc aaagggcaac gcattgccga aatgaccgcc
    29881 gcgaaagcag aacaactgat tgagcagggc attattactg acggcatgat agtgaaagtg
    29941 aacgcggcgc tggatgctgc gcgcacgttg ggccgtccgg tagatatcgc ctcctggcgt
    30001 catgcggagc agcttccggc actgtttaac ggtatgccga tgggtacgcg gattttagct
    30061 taagttttgt tggccggagg cgcagctttc cggcattgaa tttcaaaata aggaaacaga
    30121 gttatggcac tttggggcgg gcgttttacc caggcagcag atcaacggtt caaacaattc
    30181 aacgactcac tgcgctttga ttaccgtctg gcggagcagg atattgttgg ctctgtggcc
    30241 tggtccaaag ccctggtcac ggtcggcgtg ttaaccgcag aagagcaggc gcaactggaa
    30301 gaggcgctga acgtgctgct ggaagatgtt cgcgccaggc cacaacaaat ccttgaaagc
    30361 gacgccgaag atatccatag ctgggtggaa ggcaaactga tcgacaaagt gggccagtta
    30421 ggcaaaaagc tgcataccgg gcgtagtcgt aatgatcagg tagcgactga cctgaaactg
    30481 tggtgcaaag ataccgttag tgagttactg acggctaacc ggcagctgca atcggcgctg
    30541 gtggaaaccg cacaaaacaa tcaggacgcg gtcatgccag gttacactca cctgcaacgc
    30601 gcccagccgg tgacgttcgc gcactggtgc ctggcctatg ttgagatgct ggcgcgtgat
    30661 gaaagccgtt tgcaggatgc gcttaagcgt ctggatgtca gcccgttagg ttgtggcgcg
    30721 ctggcgggaa cggcttatga aatcgaccgt gaacagttag cgggctggct gggatttgct
    30781 tcagcaaccc gtaacagtct cgacagcgtt tctgaccgtg accacgtgct ggaactgctt
    30841 tctgctgccg ctatcggcat ggtgcatctg tcgcgttttg ctgaagatct gattttcttt
    30901 aacaccggcg aagcggggtt tgtggagctt tctgaccgcg tgacttccgg ttcatcatta
    30961 atgccgcaga agaaaaaccc ggatgcgctg gagctgattc gcggtaaatg cggtcgggtg
    31021 cagggcgcgt taaccggcat gatgatgacg ctgaaaggtt tgccgctggc ttacaacaaa
    31081 gatatgcagg aagacaaaga aggtctgttc gacgcgctcg atacctggtt ggactgcctg
    31141 catatggcgg cgctggtgct ggacggcatt caggtgaaac gcccgcgttg ccaggaagcg
    31201 gcgcaacagg gttacgcgaa cgccactgaa ctggcggatt atctggtggc gaaaggtgtg
    31261 ccgttccgcg aggcacacca tattgtgggt gaagctgtgg tggaagccat tcgtcagggc
    31321 aaaccgctgg aggagctgcc gctcaccgag ttgcagaaat ttagtccggt gattggtgaa
    31381 gatgtctatc cgattctgtc gctgcaatcg tgcctcgaca agcgtgcggc aaaaggcggc
    31441 gtctcaccac agcaggtggc gcaggcgatt gcttttgcaa gggcgcggtt ggggtaagaa
    31501 catttatatg tacaaatttg agcctggctt atcgccgggc ttttttatgg caaaaaaaag
    31561 cggatcctgg agatccgcaa aagttcacgt tggctttagt tattcgagtt gagaaactct
    31621 cgaaacgggc agtgacttca agggttaaaa gaggtgccgc tccgtttctg tgagcaatta
    31681 tcagtcagaa tgcttgatag ggataatcgt tcattgctat tctacctatc gccatgaact
    31741 atcgtggcga tggaggatgg ataatgaata ttcgtgatct tgagtacctg gtggcattgg
    31801 ctgaacaccg ccattttcgg cgtgcggcag attcctgcca cgttagccag ccgacgctta
    31861 gcgggcaaat tcgtaagctg gaagatgagc tgggcgtgat gttgctggag cggaccagcc
    31921 gtaaagtgtt gttcacccag gcgggaatgc tgctggtgga tcaggcgcgt accgtgctgc
    31981 gtgaggtgaa agtccttaaa gagatggcaa gccagcaggg cgagacgatg tccggaccgc
    32041 tgcatattgg tttgattccc acggttggac cgtacctgct accgcatatt atcccgatgc
    32101 tgcaccagac ctttccaaag ctggaaatgt atctgcatga agcacagacc caccagttac
    32161 tggcgcaact ggacagcggc aaactcgatt gcgtgatcct cgcgctggtg aaagagagcg
    32221 aagcattcat tgaagtgccg ttgtttgatg agccaatgtt gctggctatc tatgaagacc
    32281 acccgtgggc gaaccgcgaa tgcgtaccga tggccgatct ggcaggggaa aaactgctga
    32341 tgctggaaga tggtcactgt ttgcgcgatc aggcaatggg tttttgcttt gaagccgggg
    32401 cggatgaaga tacacacttc cgcgcgacca gcctggagac gctgcgcaac atggtggcgg
    32461 caggtagcgg gatcacttta ctgccagcgc tggctgtgcc gccggagcgc aaacgcgatg
    32521 gggttgttta tctgccgtgc attaagccgg aaccacgccg cactattggc ctggtttatc
    32581 gtcctggctc accgctgcgc agccgctatg agcagctggc agaggccatc cgcgcaagaa
    32641 tggatggcca tttcgataaa gtgttaaaac aggcggttta aaccgtttaa cgcagctacc
    32701 cgataggctt ccgccatcgt cgggtagtta aaggtggtgt tgacgaagta ctcaatagtg
    32761 ttgccgccac ctttctgttc cataatcgcc tgaccgatat gaataatttc ggcagcgcgc
    32821 tcgccaaagc agtgaatgcc cagaatctct tttgtttccc gatggaacaa aattttcagc
    32881 gtgcccacgt tcatgccgac gatttgtgca cgtgccagat gtttgaactg ggcgcggccc
    32941 acttcatatg gcactttcat cgcggtcagc tgctgttcgg ttttgcccac agagctgatt
    33001 tccgggatgg tgtaaatgcc ggtagggata tcttcaatca gatgcgcggt ggcttcgcct
    33061 tttaccagcg cctgcgcggc aatgcgcccc tggtcatagg ccgctgatgc caggctcgga
    33121 taaccaatca cgtcgcccac cgcgtaaacg tgcggctgtg cggtctgata catgctgttg
    33181 accttcagct gtccgcgact gtcagtttcc agcccaatgt tctgtaacgc cagcgaatcg
    33241 gtattaccgg tacgaccgtt ggcatagagc aggcagtcag ctttcagttt tttacccgac
    33301 ttcagatgca tgatcacacc gtcgtcacag ccttcgatct tctcgtactc ttcgttgtga
    33361 cgaatcacta cgccactgtt ccagaagtga taggagagag aatctgacat ctcttgatcg
    33421 agaaatgcca gcaggcgatc gcgggtgttg atcagatcca cttttacatc cataccgcgg
    33481 aagatcgacg catattcaca gccgatcact ccagcaccat agataagtac atggcgcggt
    33541 tcgtggtgca tgctgagaat tgagtcgctg tcgtaaatgc gtggatgggt gaaatcaaca
    33601 tctgttggat gatatggacg agagccgcag gcaataacaa atttttcagc ggttagtgtt
    33661 tcaacgctgc cgtccgggca atccagcgcc aacgtatgct cgtcaacaaa gcgagcgttc
    33721 ccctgcaata tttcacagtg attacgttcg taaaatccct gacgcatgcg cgtttgttga
    33781 ttaatcacgt tatcggcatg gttaaggata tcggcaaaag aagagcggag cagtcgggaa
    33841 tggtcgctgt atagtgggtt ttgattgaat tctataatgc ggctgacggc gtgacggaga
    33901 gctttcgacg ggatggtgcc ccagtgggtg caaccgccgc caacattttg ataacgctcg
    33961 ataactgcga cgcgcgcacc ttgcttaacc aggcccattg cagcgccttc gccgccgggg
    34021 ccggaaccta ttactatggc atcgtaatcg taggaatgtg gcatggtagg gcttacctgt
    34081 tcttatacat aaaagcaaca gaatggtaac attttatcgc gggtaagcca attgatcccc
    34141 gtcattaatc tggctatagc gtgagcggcc tttgctttgt ctgcctcctt cttaacccat
    34201 ttattcacta ttaatcgctt tttattatgg ctgatagttt tatcttgccg aagcgtgctt
    34261 ttttgttatt gtctttgtga aatc
//
LOCUS       DAZFAB010000040        33600 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZFAB010000040 DAZFAB010000000
VERSION     DAZFAB010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33600
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.040"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(280..1964)
                     /gene="treA"
                     /locus_tag="ACHJWA_002264"
                     /pseudo
     CDS             complement(280..1964)
                     /gene="treA"
                     /locus_tag="ACHJWA_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
     gene            complement(2284..3702)
                     /gene="dhaM"
                     /locus_tag="ACHJWA_002265"
     CDS             complement(2284..3702)
                     /gene="dhaM"
                     /locus_tag="ACHJWA_002265"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFO4308858.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDNCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGAEIDKVIFDAMHALEAKREQLGLPSSDTEISDSCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSPL
                     TVEEEQERLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSPVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEEKLTLDVKTQRFNRQG"
     gene            complement(3713..4345)
                     /gene="dhaL"
                     /locus_tag="ACHJWA_002266"
     CDS             complement(3713..4345)
                     /gene="dhaL"
                     /locus_tag="ACHJWA_002266"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFO4308859.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSCVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPAALEAASS
                     IAEFAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(4356..5426)
                     /gene="dhaK"
                     /locus_tag="ACHJWA_002267"
     CDS             complement(4356..5426)
                     /gene="dhaK"
                     /locus_tag="ACHJWA_002267"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFO4308860.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQVDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     GVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            5654..7573
                     /gene="dhaR"
                     /locus_tag="ACHJWA_002268"
     CDS             5654..7573
                     /gene="dhaR"
                     /locus_tag="ACHJWA_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFO4308861.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCVTPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            complement(7673..10558)
                     /gene="ycgV"
                     /locus_tag="ACHJWA_002269"
     CDS             complement(7673..10558)
                     /gene="ycgV"
                     /locus_tag="ACHJWA_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO4308862.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAILFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSSIKTSGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNKGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPDPTPNPTPTPKPTTTADAGGNYLNVGYLLNYVENR
                     TLMQRMGDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMP
                     LYVGLYIGSTHASPDYSGGDGTARSDYMGMYASYMAHNGFYSDLVVKASRQKNSFHVL
                     DSQNNGVNANGTANGLSISLEAGQRFNLTPTSYGFYIEPQTQLTYSHQNEMAMKASNG
                     LNIHLNHYESLLGRASMILGYDITAGNSQLNIYVKTGAIREFSGDTEYQLNNSREKYS
                     FKGNGWNNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYHFSF"
     gene            complement(11402..12493)
                     /gene="ychF"
                     /locus_tag="ACHJWA_002270"
     CDS             complement(11402..12493)
                     /gene="ychF"
                     /locus_tag="ACHJWA_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFO4308863.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(12610..13194)
                     /gene="pth"
                     /locus_tag="ACHJWA_002271"
     CDS             complement(12610..13194)
                     /gene="pth"
                     /locus_tag="ACHJWA_002271"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFO4308864.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            13472..13750
                     /gene="ychH"
                     /locus_tag="ACHJWA_002272"
     CDS             13472..13750
                     /gene="ychH"
                     /locus_tag="ACHJWA_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFO4308865.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(13805..15484)
                     /gene="dauA"
                     /locus_tag="ACHJWA_002273"
     CDS             complement(13805..15484)
                     /gene="dauA"
                     /locus_tag="ACHJWA_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFO4308866.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(15609..16556)
                     /gene="prs"
                     /locus_tag="ACHJWA_002274"
     CDS             complement(15609..16556)
                     /gene="prs"
                     /locus_tag="ACHJWA_002274"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFO4308867.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(16707..17558)
                     /gene="ispE"
                     /locus_tag="ACHJWA_002275"
     CDS             complement(16707..17558)
                     /gene="ispE"
                     /locus_tag="ACHJWA_002275"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFO4308868.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGANLS
                     PLHRAML"
     gene            complement(17558..18181)
                     /gene="lolB"
                     /locus_tag="ACHJWA_002276"
     CDS             complement(17558..18181)
                     /gene="lolB"
                     /locus_tag="ACHJWA_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFO4308869.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            18395..19651
                     /gene="hemA"
                     /locus_tag="ACHJWA_002277"
     CDS             18395..19651
                     /gene="hemA"
                     /locus_tag="ACHJWA_002277"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFO4308870.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSDIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEQVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            19693..20775
                     /gene="prfA"
                     /locus_tag="ACHJWA_002278"
     CDS             19693..20775
                     /gene="prfA"
                     /locus_tag="ACHJWA_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFO4308871.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            20775..21608
                     /gene="prmC"
                     /locus_tag="ACHJWA_002279"
     CDS             20775..21608
                     /gene="prmC"
                     /locus_tag="ACHJWA_002279"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO4308872.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALARERPDCEITAVDRMPDAVALAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            21605..21997
                     /gene="sirB2"
                     /locus_tag="ACHJWA_002280"
     CDS             21605..21997
                     /gene="sirB2"
                     /locus_tag="ACHJWA_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFO4308873.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLTLVVLYIIIKLATTKVPLLG"
     gene            22001..22810
                     /gene="sirB1"
                     /locus_tag="ACHJWA_002281"
     CDS             22001..22810
                     /gene="sirB1"
                     /locus_tag="ACHJWA_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFO4308874.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            22846..23700
                     /gene="kdsA"
                     /locus_tag="ACHJWA_002282"
     CDS             22846..23700
                     /gene="kdsA"
                     /locus_tag="ACHJWA_002282"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFO4308875.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLRQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(23848..23955)
                     /locus_tag="ACHJWA_002283"
     CDS             complement(23848..23955)
                     /locus_tag="ACHJWA_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO4308876.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(24384..24491)
                     /locus_tag="ACHJWA_002284"
     CDS             complement(24384..24491)
                     /locus_tag="ACHJWA_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO4308877.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(24890..25990)
                     /gene="chaA"
                     /locus_tag="ACHJWA_002285"
     CDS             complement(24890..25990)
                     /gene="chaA"
                     /locus_tag="ACHJWA_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFO4308878.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            26260..26490
                     /gene="chaB"
                     /locus_tag="ACHJWA_002286"
     CDS             26260..26490
                     /gene="chaB"
                     /locus_tag="ACHJWA_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFO4308879.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            26651..27346
                     /gene="chaC"
                     /locus_tag="ACHJWA_002287"
     CDS             26651..27346
                     /gene="chaC"
                     /locus_tag="ACHJWA_002287"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFO4308880.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     RPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(27390..27743)
                     /gene="ychN"
                     /locus_tag="ACHJWA_002288"
     CDS             complement(27390..27743)
                     /gene="ychN"
                     /locus_tag="ACHJWA_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFO4308881.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            27929..29323
                     /gene="ychO"
                     /locus_tag="ACHJWA_002289"
                     /gene_synonym="iatA"
     CDS             27929..29323
                     /gene="ychO"
                     /locus_tag="ACHJWA_002289"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFO4308882.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(29324..29974)
                     /gene="narL"
                     /locus_tag="ACHJWA_002290"
     CDS             complement(29324..29974)
                     /gene="narL"
                     /locus_tag="ACHJWA_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFO4308883.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(29967..31763)
                     /gene="narX"
                     /locus_tag="ACHJWA_002291"
     CDS             complement(29967..31763)
                     /gene="narX"
                     /locus_tag="ACHJWA_002291"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFO4308884.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            32102..33493
                     /gene="narK"
                     /locus_tag="ACHJWA_002292"
     CDS             32102..33493
                     /gene="narK"
                     /locus_tag="ACHJWA_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFO4308885.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
BASE COUNT         8198 a         8328 c         8595 g         8479 t
ORIGIN      
        1 catgtttgat gtcaaaattt gtctaaagag acagaatgat ttgcttgttg cttaaataac
       61 actttgttcc acaagaaaat tgtcagggag aatggcggag tggcgaaaaa tataaataga
      121 ggtgtactga ttaacaaatt gataaaataa aataaaatta aaatcttctc tgatataaga
      181 aaataaaagc atagaccgta gaatgggggg catttcgtgg cgaaaaaaga gcaatgccaa
      241 ttttatgcac ataatggcat tgctcgatat acagcaaagt taaggcgtag gttccgcctc
      301 tttttgtttt accggctgtg ttgttgattc actgagtgga cgcgtcgccg gaacattgtc
      361 acacggttgc tctttcgggc agatcaaatc cagcattttc agcgtcacgc cattggtcca
      421 gccaaagcca tcctgtaatg gatattcgcc accgccgccc cccgttccgg tggtgctgac
      481 atcatatttt tccaccagct ttttctcccg gtcataggtg tgctgaacat tggtcaggaa
      541 gtgccagcta atgtccatcg ccacctcttt ttgcccgtag ttttgtaatc cttctgtcgc
      601 gacccactgt aacggtgccc agccatttgg cgcatcccat tgttgcccac ttttcaccga
      661 cgtggtgttc aggccgccgg gttgcagcag atgagttttc gtcgccgtcg ccattttgtt
      721 ggcgcgatct ttcgctgccg cattgacgta cagtgggaac agggcggccg cggttaactg
      781 attgcgcact ttatgacttt tcaggtcgta atcggcatac cagccttgtt gatcgttcca
      841 caggtatttt tcgatccctt tttgacgggc atttgccagt gtttcgtact ggtttgccat
      901 cgcgttatct ccggcagctt tgctggcgcg ggcaaggatt ttttccattt tgaacatcag
      961 gctgttcaga tcgaccggta cgatgctggt ggtgcgtaag gtatttagtt gctgcggatt
     1021 gtccatccag cgcgagctga aatcccagcc agacgcagca gcagagcgca ggtcgcggta
     1081 aatttcagtg gcaggtcgat tcggattgct tttagcggtg gcaatatctt ccacccatga
     1141 ctctggtcgt ggcgtatcgc gatcgtccca gtagcggttg agaagggtac catcctgaag
     1201 ttttacaacg cgtttttcct gttgtccggc ttgcaggttt tcaacaccgt ccatccagta
     1261 agcatattct ttttgcattt gcggcaggta ttgcttcaat gcggcatcgc cttcatgctg
     1321 cgccagtaac tctaccatca gggcaaagaa gggcggctgc gagcggctta aatagtaact
     1381 gcggttgccg ttgggaatat gaccgtaagt gtctatttca tgagcaaaat tggccaccat
     1441 atccgcgact ttatcccagt gaccgctttc ggcaagtcct aacatggtga agtaactgtc
     1501 ccagtaatat acctcgcgaa aacgtccgcc tggcacgaca taaggttccg gcagcggtaa
     1561 cagagaatcc catttttcgg tgttttcggt agaacgcgtt aataccggcc aaagtccgtc
     1621 aatatgttcg cgcagtgact gtccctctgg cggaacatat ttctcgccct ctttcggcag
     1681 ggtgaaattg acgttaacga aatggcgcag atcaaatccg ctctggtttt gctgcatccg
     1741 atcatcagcg gatcgctgtt cggcacggca tcggcaaagg ttttttggtc cggaaaaagt
     1801 ttggcgtttt gcacatcatt aaacagcggc cctaataaaa tatcaggcgg ctgtggcgtt
     1861 accggtgttt cttctgcctg caccgatagc gcagcgaaac acaaaaagat gcaggctgga
     1921 attaacgcca ttttttgcgg gcgagaaggt gcgggggatt tcatcaatca ttctccttag
     1981 gcgaaaccga ataaaacggt attcaagtat cagaaaacct tagttcagga tcgcactagc
     2041 tgcatgatcg aaatctcatt ctgcgaactg tcaggcattt tagaaaaggg aattaaaggg
     2101 ataaataacg gcgaaataga gtgatcaaat aacgtctgtt tattagccag ccagcgtcgt
     2161 ttggttcgcg attagcgaag aaaaatcatc ataaattggt gaaaaatata acaggtagtt
     2221 aaaaaacatt agtgctgagt aaattgccgg atgacatcag aacgatgcca tccgaacggt
     2281 ggcttaaccc tgacggttga aacgttgcgt tttaacgtcc agcgttagct tttcttctgg
     2341 ttgtatcgca tacagtttct caccctgctg gcaaatccag ccaatcccca gttcacgggc
     2401 gatcagggcg ctgtgggata ccggacttcc ggcgctaagg cagatacctt ttacaaccgc
     2461 cggatccagt tgcagtactg gggagggata aatgttctcc gccagtagaa tagttggcga
     2521 gttaaactgc gggagttctt ctttcgtttg ggtcaggtgg accagggtgc gatgcagaag
     2581 atcgtccaca tcaatatagc gagcttgtag atattcatca tccagttgct ggtattgctg
     2641 gctaagttct ttaagaactt gctgccaggc atattctgcc gtgcaatgtt cgtgctgaag
     2701 gagttcgctt gccgccgcca gcagttccgg atcatctaac agtgtatggt gaccagaaaa
     2761 gattgcggca atatcgtcaa gcccgctggc ttctgctttt gctgttaacg tcatcagatc
     2821 taataacgtg aagtcaatgg cctggcgtaa tcgttcctgt tcttcttcca cggtcagggg
     2881 tgattttgcc tgtaccgtac ataaaactgg ttgataataa aaggctttac ccgaaacagg
     2941 cggaacggga cgcagagtag gtggagcaac ttcctccgtt tcaccaaagt tatcttcagc
     3001 cagctgacgg aaagcgatca gtgcctcttc agcttctggc cctttcgcaa tcaggcgcag
     3061 cgtatcgtta tagcgaactt gtagtaacgc aatctggtta atactctccg gtgtgacgca
     3121 tttgccgttt ttttccagca acatatcggc attaaatgtc gataaggtat aaaccagccg
     3181 ggaggccgga cggacgtgca ggccgttacg gtttttaatg accaccgcca gagaacgggc
     3241 ttcttcatcg tacgcaggac atgagtcaga gatttcagtg tcggaggacg gtaaacccag
     3301 ttgttcacgt ttggcttcca gcgcatgcat ggcgtcaaag ataactttgt cgatctccgc
     3361 ccccgaggcc gcgctgacag ttgctgccag tgtaccttcg accaacggcg cagcacacaa
     3421 acgtactttt gcggcgatct cgggagccag caattccagc gcagtttcag cactcaataa
     3481 tgcgctaccc atatccatca tgaccagcac atggtcggca tcagcaacag attcgatggc
     3541 ctccatcact ttgacggcat cggtaccaat gggattttgt ggatcgtcaa ttcccgcagc
     3601 aatggcgatt ttacaattat cactcattaa catctgacgg gctaattcac cgacaccttc
     3661 tcccagtcgg ctgctatgtg aaactatgac caggtttacc atcaccaatt ccttactctt
     3721 ttgcggctaa cgccaacatt tgcatcataa acatcaccga ggttgcgccg ggatcctggt
     3781 gaccaatact gcgttcaccg agataactgg cgcggccttt gcgggcttgc atcgtaatcg
     3841 tactttgtgc agcgaattcg gcgatgctac tggcagcttc gagcgccgcc ggaacagaga
     3901 gattttgctc gctggactga cgtaacgatt ccaccaccgg cacccacaca tcacacatgg
     3961 ttttatcgcc aggttcggct ttcccgcgac tgattacgcc gtccgcgcca tcgcggaaca
     4021 tctgataaag ctcttccagc gtcaggcttt gccgtgcctg ggtcgcctgt gcggcgcgga
     4081 taaagaaggt accgaacagc ggaccactgg caccaccgac gcaggaaagc agcgtcatac
     4141 cggtattctt gagaatgaaa ccgatatctt tatctgcaat agcagggagt ttttccacca
     4201 ctttgctaaa gcctcggttc atatttagcc cgtggtcagc gtcgccaatc tcgcgatcca
     4261 gtccggtaag atactcgctc tcggtgttga aaatatcgcc acaacgagtg agccagttaa
     4321 caatttgagt tctgctcagt gacattgctt tctccttatt taccccagtt aagggccggg
     4381 gtgtggaccg gggcgtccca gagtgccagc gtttcgtcat caactttcag taaagtgatt
     4441 gagaaaccgg tcatatccag tgaggtgcag tacgcgccaa ttaaattacg ctcgatagtc
     4501 aatcccgctt gctggcaacg tgtggtcagg cggttataga cgccgtacag ctcagaaagt
     4561 ggagttgcgc caagattgtt aaccagcgca atcaccccgt cgccagactg gagcggttgt
     4621 ttggtttgtg gttcttcctg ccaactgcct tgttgataat cccagaaacg caaagtgcga
     4681 tggtatgagc cattttccag cagggtgtcg aacatttcat cgacggtttg atcaagggaa
     4741 gagaaggggc gacggtcaat acccggctca ccatgaatgc cgacgccaaa ctccatctca
     4801 ttatccgcca gggtaaaaga aggtttgccc gcggcaggaa cggtacaggc accgagagcg
     4861 atacctattg agtggccttg attattcaac ttacgcccca gttccgcaca ggcgtccagt
     4921 gagtcgccac gctccgccgc tgcgcctacg agtttttcaa ttaatacggt gttggcaacg
     4981 ccgcgccgcc cggcggtata aagactgtct tttactgcaa cgtcgtcatc aatgaccaca
     5041 gtggtcactt ttacgccgct atcgtgcagt aactcggtcg ctgtttcaaa gttaagaata
     5101 tcgccggtgt aatttttgat aatcaacagt acaccttcgc cgccatcaac ttgcatggcg
     5161 cattcaaaga ttttatcggg cgtcggtgag gtgaaaattt cgcccggaca ggcccccgaa
     5221 agcatcccct gaccgatata accacagtgc atcggctcgt gtccgctgcc gccacccgac
     5281 agcagggcga cttttcctgc aacaggggca tcagctcggg tgacatacac cggatcctga
     5341 tgcagtgtca gcgatggatg cgctttcgcc agtcctgcca gttgttcgtc cagtacgtct
     5401 tgcacatcat tgatcaattt tttcattatt ttgctccagc aattacggta gggcatggat
     5461 gatgttcaac gacacggcga ccactgactg ccgatgaatc cattgtgcat caggcaaggg
     5521 aaaagaaaat tagcccaaat tatgtttcat agtgaaacat atgctttaat gaatgttcca
     5581 tattgaaact tttacgtgta ttaatactta aaattgcgag ccggaacacc ttttgtcata
     5641 agggatgcgg gatatgagtg gcgcttttaa caacgatggt cggggcatat ctcccttaat
     5701 tgcaacctcc tgggagcgat gcaataagct gatgaaacgg gagacatgga acgtaccaca
     5761 tcaggcccag ggcgtgacat ttgcttctat ttatcggcgt aagaaagcga tgctgacgct
     5821 cgggcaggct gcgctggaag atgcctggga atatatggct ccgcgagagt gtgcgctgtt
     5881 tatcctcgat gaaaccgcct gcattctcag ccgtaatggc gatccgcaaa ccttgcagca
     5941 gctaagtgca ctgggattca atgacggcac gtattgcgcc gagggaatta ttggtacttg
     6001 tgcgctatcg ttagcggcta tctctggtca ggccgtgaaa acgatggccg atcaacattt
     6061 caaacaggca ctctggaact gggccttttg tgtaacgccg ttatttgaca gcaagggccg
     6121 attgacggga acaatagcgc tggcgtgtcc ggttgagcaa actaccgcag ctgatttgcc
     6181 gttgacgttg gcaatcgccc gcgaggtcgg aaatttactg ctgacggaca gtttgctcgc
     6241 tgaaactaac cgtcatttaa atcaacttaa tgccctgtta gaaagtatgg atgatggcgt
     6301 gattagctgg gacgagcagg gtaatttgca atttattaat gcccaggcgg cgcgggtctt
     6361 gcgccttgac gcgacggcaa gtcagggacg ggcaatcact gaactcttaa cgttacccgc
     6421 cgtattgcaa caagcaataa aacaggcaca tccgctcaaa cacgtagaag caacctttga
     6481 aagccagcac cagtttattg atgcggtgat aacccttaaa ccgataatag aaacgcaggg
     6541 aaccagcttt attttgttgc tccatcctgt ggaacagatg cggcagttaa tgaccagtca
     6601 attaggaaaa gtcagccata ccttcgctca tatgccacag gacgatccgc aaacccgccg
     6661 cttgattcat tttggtcgcc aggcggcgcg cagtagcttt cctgtcctgc tttgtggaga
     6721 agagggcgtg ggcaaggcac tgctaagtca ggcaattcat aatgaaagcg agcgtgctgc
     6781 aggtccttat atcgccgtca attgtgagtt atatggtgat gcggcactgg cggaagaatt
     6841 tattggtggc gatcgcacgg acaatgaaaa tggccgtctg agtcggctgg aactggcgca
     6901 cggcggcacg ctgtttcttg aaaagattga atatctggcg gtggagttac agtctgcttt
     6961 gcttcaggtt atcaagcagg gtgttatcac gcgactggat gcgcggcgtt taataccaat
     7021 tgatgtcaaa gtgattgcta caacgaccgc ggacctcgca atgctggtgg aacaaaatcg
     7081 ttttagtcgc cagctgtatt acgcgctgca tgcatttgaa attaccatcc cgcctctgcg
     7141 tatgcggcgt ggcagcattc cggctctggt gaataacaaa ttacgcagtc ttgaaaaacg
     7201 cttctctacg cggctgaaaa ttgatgacga tgccctcgct cgcctggttt cctgtgcatg
     7261 gcctggcaac gattttgaac tttacagcgt catcgagaat cttgctctga gtagtgataa
     7321 tgggcgcatt cgcgtcagtg atttgccgga acatctgttt accgagcagg cgacagatga
     7381 tgtcagcgcc acacgccttt ccaccagtct gtcatttgcg gaagttgaaa aagaggcaat
     7441 tattaacgca gcccaggtca caggcggtcg cattcaggaa atgtcggctt tacttggaat
     7501 cggccgcact acgctgtggc ggaaaatgaa gcaacatggc attgatgcag ggcagtttaa
     7561 gcgccgggta tgaaagacag agacgatttc tgatacatca gagtgatcta tatttcattc
     7621 cggcgcacgc taacattttt cagcatcgtt taagggcttg tctaccccgt acttaaaagc
     7681 tgaagtgata tcctccgttg acttgcttct gatcaaagag gttaccctgc gtgtaatccg
     7741 cttcgaggta gaatgtgtgc tgtttgttat actgtgcact gactcccacg ccgttattcc
     7801 agccattacc tttgaaactg tacttctccc gggagttatt caactgatat tctgtatccc
     7861 ctgaaaactc gcggatagcg ccagtcttca catagatatt cagctggctg ttgcctgcgg
     7921 tgatgtcata tcccagtatc atgctggcac gccccagcag cgattcgtag tgattcagat
     7981 gtatattgag accattactc gccttcatag ccatctcatt ctggtggctg tatgtgagct
     8041 gggtttgcgg ctctatatag aacccataac tagtaggggt caggttgaac ctctgcccgg
     8101 cttccaggga gatgctcaat ccattcgcag tgccgttggc gttaacgccg ttgttctgac
     8161 tgtccagtac gtggaaacta tttttctggc gcgatgcttt tacaacgaga tcgctgtaaa
     8221 aaccgttgtg tgccatgtaa ctggcgtaca ttcccatgta gtctgaacgt gcggtaccgt
     8281 cgccaccgct atagtccggc gatgcatgtg ttgagccaat atacagaccg acatacaacg
     8341 gcattacatc agagagacgt ttatccccac caaactggat accgctgtaa cccatgtcaa
     8401 agccgctcag tttgccactg gcgaaggagt ccaggcttcc cccataactg cgcagccaga
     8461 tattaccgtc tttactctga tttcgcaggt cacccattcg ttgcatcagc gtacggtttt
     8521 caacatagtt taataagtaa ccgacattga gataattgcc gccagcatct gcggtcgtgg
     8581 tgggtttagg tgtgggagtg gggttaggcg taggatcggg ttcaggcgta ggatcggggt
     8641 tgactatggg aggctgagcg ggagctggtg ttggttcagg attaggagtc ggttccggaa
     8701 ctgtcccgga agcgtaaagc tcccagttag tgccattttt acgcacatcg tacagatatc
     8761 cccccaactc aacctgagaa gacgcgctga acgaggccgc accgtcagtg gtttttacca
     8821 ctgtcagaac ttcatttccc gttgtggcct cgctgccctg gttgcggata gccaatacgt
     8881 gattaccagc actactcccg ctgatattca ataaatcccc tttattatta acgccattac
     8941 cctcgccaac aacatcagca cgcataataa aggtactgtt accgctcagg ttctctacgt
     9001 ttaatgtggt aaatgtgccg gcagttgacc cgtggctggc aaaatcgaca gttgaatggc
     9061 tcagcgccag cgtgtcgaga ttagaattac ttgttacgtt ccagacgctg ttattcattg
     9121 caacgtccag tttcccgcca ttgacattat cgctgaggga ggaacctgtc caaaccgacc
     9181 cagggtgcat atccagattg attagcccac ctttggaaag aacgctaccg ttgataagca
     9241 tacgacccga ggcgttgatg cggctggcgg cataaccatc gtcatgttgc gttgcgatgg
     9301 ccatctggtc gggtgtactc atatcaatga ccagatcgct cgtgaggtcg atctggctgt
     9361 tggtcatggc ataaatcccc ctggctcctg ccgcgccggt gatagccaaa ctgtctccgg
     9421 ttattctacc gccgctgagc gcccacaaac ccagcgccag actgccatta cgatcaaccg
     9481 taatatcggt attttgcatg ttgataacag ccgttgccgt ctgggccgag gcaccataag
     9541 aaccgccgga aaagatgctg tttcgttgcg ctgccgtgcc agaaaaattg attgtcgcgt
     9601 ctgaaccact ggtgacgaga ccaccgccct gcgcggaaga aatatggcta tctgcaccga
     9661 tagtggttgt accaccacga acttcgacgc cattggccgc ggctccagtt acatcaacag
     9721 tgagtgcatt cgcgctaacc tggccaaagc tccagaggcc gtgtgcatta tcgccactgg
     9781 ttttaatgga actgtttgtt ccgagatcga caacagagtt tttctgtacg tttatcccca
     9841 tggcgctgta gccctgaaca tcgattgtca ggtctgtcgc cgtaaaacgc gcagcaccat
     9901 tggcgttatt gccgttgaga ccaccgatat aaacacctgt acttccatcg gtcttgattt
     9961 tacttccgct tccaagatcg acactggtgc catagtcatt gatggttagg cctataccgt
    10021 tcgagttttc aatggtgaat tgagtggctg ttagtgttga gctatgccca ataatgatgc
    10081 cgtcatcgtt actcttaatg gtgctgcctg tgcctaagtc agcatgggta tagtcaccaa
    10141 ttaaattaat gccgatggca gaggtttgac caacaacatc tactgtgagt cggttagctg
    10201 tcagggagct gtttttgccc tgaataatga ttccttttgc atatccagag gcgtcgttga
    10261 cattgacggt gacatcatta cccaggttga tattgccagg cgtgtttcct gctggggtca
    10321 ttacaccata gaggtttcct gatggatcgg cggtatctcc agtaattctg tcaccatcat
    10381 taatagtaat attggtgcca tattgacttc ctgtatcagc agctatcagc aggggggaaa
    10441 agagaattgc attcaaacca atagcgccaa attttatcag ttgaattgag ggcgaacgga
    10501 tttttaattc agcgagacga tcggctttgg tattcccatt gtgttgtttg atgcccatac
    10561 cactcctata tagtacccag gctatgggtc tatggatttt gtcattttgt ttaagtcaat
    10621 gaataatatc ctcttatcat catgatattt gtatggtgca atcatgatga gcttctctga
    10681 gaataattct ctattaccgg gcaaaatcag aaacacagaa attaatatat atttaacatc
    10741 ccccttacaa aggtgagttt agatcgagtt attccctaag ttgtcgttac ttattccaat
    10801 tcgttactat taatatgaca aaaacattac accaaacagc ggttaaataa tttggtgaat
    10861 atttttggta ataatcctaa ttagttatgg gattttcgct tgatgtatct gcataacaaa
    10921 aacaatatgc gccatttttg cattactaat aagaaaaagc aaaaaacatc gatattcatt
    10981 tttggtcaat gggttttctg gtctatcgtt gttattgtgc tactggttat cattttcatc
    11041 ttgccaggat cgcacgtaat cacgttattc ggggacttta tctgcgggat attacccttc
    11101 aggtaagtcc ttttctcgcc agcatattgg agcgtggtca atattgatcg gctgtgaagg
    11161 gggctgatgt gtagtggaac aaaaactgac gttatatcac tgctaatgag atatccgtca
    11221 tctgaacact gatgtcttta attattctcg tggttcactg taggcaataa gcacaaaagt
    11281 gtaggatgtt acaagaatga ttaggactcg gtgaaatgaa aaatccacgc aattgcgtgg
    11341 attttatata cttttgcgct cttcatgaga tttagcgaaa cctcatgaga caataaatta
    11401 attagacgtt gaaaaggaag ttcatcacat cgccatcttt cacgatgtaa tctttacctt
    11461 ctgcacgcat tttgcctgct tctttcgcgc cttgttcacc tttgtaagtg atgaaatctt
    11521 caaacgagat ggtttgtgca cggataaagc ctttttcaaa atcagtatgg attttgcccg
    11581 ctgcctgcgg cgcggttgct ccaaccggaa tggtccatgc acgcacttct ttcaccccag
    11641 cggtgaagta agtttgcagg ttcagcagct tataaccggc acggatcaca cggttcagac
    11701 ccggctcttc cagcccaagc tcctgcataa actcgtcacg ttcttcgtcg tccagttcgg
    11761 caatgtctgc ttcaacagca gcacaaaccg gaaccacaac agaaccttct ttcgccgcga
    11821 tttcacgcac ctggtcaaga tatgggttgt tttcaaaacc gtcttcgttg acgttggcga
    11881 tgtacattgt tggtttcagc gtcaggaagc tcaggtaacg aatagccgct ttctcttcag
    11941 cgcttaaatc cagcgcgcgc agcatacctg cgttttccag ctggggcagg catttttcca
    12001 ggaccgccag ctcagctttc gcgtctttat cgccaccttt ggctttcttc tgtacgcgat
    12061 gaatcgcacg ttcgcaggtg tcgaggtctg ccagcgccag ttcggtgttg ataacttcaa
    12121 tatcgtcagc cgggttaact ttgccggaaa cgtgaatgat gttgtcattt tcaaagcagc
    12181 gaacaacgtg accgatcgct tcggtttcac ggatgttggt caggaactgg ttacccagac
    12241 cttcgccttt cgatgcgcct tttaccagac cggcgatatc gacaaattcc atggtcgtgg
    12301 gaagcgtacg ttgcggtttt acgatttcag ccagttgatc caggcgagga tcaggcattg
    12361 gtacgacgcc tgtgttcggc tcaatggtgc agaatggaaa gttggccgct tcaataccgg
    12421 ctttggtcag cgcgttgaac agggtagatt tcccgacgtt gggcaaaccg acgataccgc
    12481 atttgaatcc catgatttaa atcaccttaa tatcttaata atcaacctgt tattgctaac
    12541 agattgcaga aatggaaata actttgccta ttatacacgg cactcggcaa aaatgccgca
    12601 gacaacgact tattgcgctt taaaggcgtg caatcggttc gttgctttgg tcaagccatc
    12661 tgtaaaccac atttcagtac aacgcgccgc ttcgtcgatg gcttcatcaa ttaacttctg
    12721 ttcactaaca ggcggtttgc ctaacacaaa accaacaact ttgtttttat cgcccggatg
    12781 accgattccg atgcgtaaac ggtgaaagtt agggttatta cccaatttac tgatgatgtc
    12841 tttcagtcca ttgtgaccac catggccacc gcccaactta aatttggcga cgccaggagg
    12901 cagatccagt tcgtcgtggg ccaccagaat ttcgtccgga ttaatgcgga aaaaactggc
    12961 catcgccgca acggctttgc cgctgagatt cataaatgta gtcgggacta acaggcggac
    13021 atcttcgcct ccaagggtga ctcgcgaagt ataaccaaag aatttagcct cttcgcgcag
    13081 cggagcgcgc aaacgctctg ccagtaagtc aacgaaccag gcaccagcat tatgtcgcgt
    13141 tgcggcgtat tcagcaccgg ggttcgccag gccgacaatc aatttaatcg tcacgttttt
    13201 ttgtcctgag tgtgtacata actggcgcgt agtttactgg ttgcggcccc gcttgacaaa
    13261 aaactgcgta tcaaatgcag ataacgtaat aattgcctga gtggactatt agaaagtcaa
    13321 ggtgttcagg cgtttatttg taaagttttg ttgaaataag ggttgtaatt gtgatcacgc
    13381 ccgcacataa cccacagggt gttgtctata ctttacacat aaggaagagg ggtattccct
    13441 gttacaaccc agaaagttcc ggaggtgaca tatgaaacgc aaaaacgctt cgttactcgg
    13501 taacgtgctc atggggttgg gtctggtggt aatggtggtc ggcgtggggt attcaatcct
    13561 caaccagtta ccacagttta atatgcccca gtatttcgca catggtgcag tgctaagtat
    13621 tttcgtcggt gccattctct ggctggcggg tgcccgtgtt ggcgggcatg aacaggtgtg
    13681 cgaccgttac tggtgggttc gccactatga caaacgttgc cgccgtagcg ataatcgccg
    13741 tcatagctaa caaataatgc agtttgctga ccagtcagtt tgcgctgact ggtcaatctc
    13801 gtacttataa atccgccatc gccgcgcgac gattcgggaa gaacgcgagg cgtcccggga
    13861 tcggttgaat gcctgcgcga gccatagtgc gcagtggctg gaattccacg ttgcacacgc
    13921 gcagttcaca tccttcgggc agacgcttca caaaacgctg gaacgcatca agaccaccag
    13981 catcaagtac cggaacggca tcccacttca gaatcacaat ccgtttgcct tcaagacgtg
    14041 actccaggtc cgtgaataag ccttcagcag cagcaaaaaa cagcgggcca ataacgcgca
    14101 gaaccaggac atcgtctgga acatctacga ctaccggtgc caggcgagtc atacgtgcga
    14161 tacgacgcat aaacagcagc gatgccagca cgatccccac gctgatggca ataaccatat
    14221 caaacagcac ggtcagcgac atgcacagca gcatgacaat gatgtcatct ttcggtgcat
    14281 gacgcagcaa gtcgaccact ttatgcgctt cactcatgtt ccacgccacc atcaacagca
    14341 gggctgccat agcggaaagc ggcagccagg agagcagcgg tgccagtacc agcagggcaa
    14401 gaataaccag aatagagtgg atcaccgccg agatagggga agttgccccg gcacggacgt
    14461 tagcggcaga acgcgcgatg gcagcggtag cggtaatacc accaaagaac ggagcgatga
    14521 tattccccag cccctgtcca accagttcgc tattcgcctt gtgcttcgtc ccggtcatac
    14581 catccagcac cacggcgcag agcagagatt cgattgcgcc gagcattgcc attgagaatg
    14641 ccgcaggcag cagtgtgcga atagaatccc aggttagcgt gaattctgaa ttaggcagat
    14701 cccacggcag caccagttgc ggtagcagtt gcggaatacc gttaccctga gaaccatcgg
    14761 ccaggacgta gtggaattgc gaaccgatgg tagcaacatg tccgccgagc aggttaacaa
    14821 tccccatcac cgcgcaacca gccagcaagg ccggaaggtg accgggtaaa cgaatgccca
    14881 gacgcggcca gaaaacaaga atacctagcg tcacaatgcc aatggcagca tcacccacat
    14941 taatggtcgg cagcgccata aataatgcgc cgactttttg tagataatgt tccgggacat
    15001 gggccatttg cagaccgaga aaatctttaa tctgcatggt accgatggtg atcccgatac
    15061 ccgaggtgaa acctaaggtg acggaaaccg gaatatactc aatcaggcga ccaaagcgtg
    15121 ccagacccat cagaatcaaa aagatccccg acagcaaggt cgcaaccagc agtcctgcca
    15181 gcccaaactg ttgcgaaacg ggatagagaa ttaccacaaa tgccgcagtc ggaccggaaa
    15241 cgctaaagcg tgacccaccc gtcagagcaa tgacaatccc cgcaacagct gcggtatata
    15301 aaccgtactg gggtgccaca ccactaccaa tagccaacgc catcgccagc gggatagcaa
    15361 taatcccgac ggttatcccg gcaatcaggt cacgggtaaa ccgtgcggca gtatattttt
    15421 ctttccagca agcgtcgatc agagcgcgga aaggcatcac atgtgaggaa aatattttgt
    15481 tcacaataat gtttcatccg tgagcgcatc atctgtcaac taaatggcag gtgaaggagg
    15541 cataggtcat acaaatggat attacagaca aaaaaacccg ccgcagcggg tctttgagcc
    15601 gggttcgatt agtgttcgaa catggcagag atcgattctt cgttgctgat acgacgaatc
    15661 gcttcggcca gcatacctga cagggtcaga gtacgcacgt tcggcagtga tttgatttca
    15721 tcgctcagcg gaatggtatc gcagacaacg acttcatcaa ttacagagtt acgcaggttg
    15781 ttcgccgcgt tgccagagaa gatcgggtga gtcgcgtacg caaatacacg tttagcacca
    15841 cgttctttca gagcttcagc agctttacac agcgtaccgc cagtgtcgat catatcatcg
    15901 accagtacgc agtcacgacc tgcaacgtca ccgatgatat gcatcacctg ggaaacgttc
    15961 gcacgcggac gacgtttgtc gatgattgcc atatcggtat cgttcagcag cttagcgata
    16021 gcgcgggcac gcacaacgcc gccgatgtcc ggagaaacca caattgggtt atccagattc
    16081 agctgcagca tgtcttccag caggatcggg ctaccaaata cgttatcaac cggaacgtcg
    16141 aagaaaccct gaatctgttc agcgtgcaga tccactgtca gcacacggtc aacaccgacg
    16201 ctggagagga agtctgcaac cactttcgca gtgattggta cacgagcgga acggacgcga
    16261 cggtcctggc gcgcatagcc aaagtagggg ataacagcgg tgatacgacc tgcggaagca
    16321 cgacgcaggg catcaaccat aacgactaat tccatcaggt tgtcgttagt aggggcacaa
    16381 gtggactgga tgatgaaaat atcaccaccg cgtacatttt cattaatttg tacgctgact
    16441 tcgccatcgc taaagcgacc tacagcggcg tcgccgagtg aagtgtacag gcggttggca
    16501 atacgttgtg ctagttccgg ggtggcgtta ccagcaaaaa gcttcatatc aggcacgaga
    16561 agaacctcag gcatgcgtcc attggtggaa agaatctgcc gaaaactgtg cgggccaggc
    16621 atgatccttt ccaggcggtg tattaaagag cgcgatgcaa cgtctggaac aaggtgacgt
    16681 tgtcaccgaa actcagcttg cccggcttaa agcatggctc tgtgcaatgg ggaaagatta
    16741 gcgcctttcg ccacaaagcc attgagccat tccggggctt gctctagcac ctggcgggct
    16801 tcagactctg tatcaaattc agcaaagaca caggcccctg tcccagtcag gcgcgacggg
    16861 gcgtattcta acagccagga aagcaccgca tcaacctcgc gaaaacgttt tcttgcgata
    16921 acctcgcaat cattgctgaa ttcacatttt agcaacgttt ctattgacct ttttggcgta
    16981 ttgcgcggga gttcaggatc tttaaaaatc accggagtcg gaatacttac accagggtgc
    17041 gccaccagat accacttctc tggcggatcc accggggtta gtatttcacc aacgccttcg
    17101 gcaaacgcgg catgcccccg aacaaagaca ggaacatctg cgcccagcgt cagccccatt
    17161 tccgccagct catccatgct tagcccgcat tgccagagat gatttaatgc caccaggacc
    17221 gtcgcggcat tggatgaacc accgccgaga ccgccgccca tcggcaaacg cttgtcaatg
    17281 ctgatattcg caccgcttcc cgtcggaaga cgcccgctgt ctgccgcagt tttcatcaac
    17341 aaccgcgctg cgcgaacgat caggttatct tcatgttcca cgccttcaac gggcgttaac
    17401 agacggatat ccccatcgtc acgaagctca atgctgatgg tgtcgccgta atcaagaaac
    17461 tgaaacagcg tttgcagcgt gtggtaacca tccgcacgct gaccggtaat gtataaaaac
    17521 agattaagtt ttgccggaga gggccactgt gtccgcatta tttcactatc cagttatcca
    17581 tttttaactt gatgcgttga ccaccgtcgg tgagttccat attggctggc atcgcaggtt
    17641 gcgttttggt gtcataacca ccataaacaa ccttccagtt tttgccattc tggctgtagg
    17701 taatttcgct caggcggtac tggtcgtcca gtttgtagtc ggttgcatca cccggtaaac
    17761 ctaaaatcca ctggcgcaag ctgttgagcg gaattggcat tccagtcaat ttgccaatca
    17821 tctcttcggc gtcatcggcg gtataacgct gacctttatt gtcgactaac tgcacgttac
    17881 ccggttgagc attcagctcc agttccgtgc tgcccaatgg gttagtgagc agcagacggt
    17941 agcgatcctg gccggtttgc tgccagaaaa agcgggcgta cactttttgt tggtcagaaa
    18001 tataagcgaa cgcgccgcga gtctgatact gattaagatt gcgcacgtct tgctgatgct
    18061 gacgccattg tggcgaatcc gggcttttgc caggaccttt gggcgtggta acggaacagg
    18121 cagtgagcac aagagcagcc agcggtagca ggcggataag acgaaaatcg ggcaggggca
    18181 tagtgatgac aagtccttga gatacgttgc agttataacc cttaatgcta gcgtcaccat
    18241 tagtcatcgt ctatgttcaa gttgtcttaa ttgtagaaat ctaacgggtt cgggcaatta
    18301 ctccaaaagg gggcgctccc ttttattgat ctcgcgcata cggtatgatg cgccgagact
    18361 aaccttatca acgttggtat tatttcccgc agacatgacc cttttagcgc tcggtattaa
    18421 ccataaaacg gcacctgtat cgctgcgaga acgtgtatcg ttttcgccgg acaaacttga
    18481 tcaggcgctg gacagtctgc tcgcacagcc gatggtgcag ggcggggtgg tgctgtcgac
    18541 gtgtaaccgc actgagcttt atcttagcgt cgaagagcag gacaacttgc aagaggcgtt
    18601 aatccgctgg ctttgcgatt atcacaatct taatgaagaa gatctgcgta aaagcctcta
    18661 ctggcatcag gataacgacg cggttagcca tttaatgcgt gttgccagcg gcctggattc
    18721 actggttctt ggggagccgc agatcctcgg tcaggttaaa aaagcgtttg ccgattcgca
    18781 aaaaggtcat atgaaggcca gcgaactgga acgcatgttc cagaaatctt tctctgtagc
    18841 gaaacgcgta cgcactgaaa cagatatcgg tgccagcgct gtgtctgtcg cttttgcggc
    18901 ttgtacgctg gcgcggcaga tctttgaatc gctctctacg gtcacagtgt tgctggtagg
    18961 cgcgggcgaa accatcgagc tggtagcgcg tcatctgcgc gaacataaag tacagaagat
    19021 gattatcgcc aaccgcactc gcgaacgtgc ccaaattctg gcagatgaag tcggcgcgga
    19081 agtgattgcc ctgagtgata tcgacgaacg tctgcgcgaa gccgatatca tcatcagttc
    19141 caccgccagc ccgttaccga ttatcgggaa aggcatggtg gagcgcgcat taaaaagccg
    19201 tcgcaaccaa ccaatgctgt tggtggatat tgccgttccg cgcgatgttg agccggaagt
    19261 tggcaaactg gcgaatgctt atctttatag cgttgatgat ctgcaaagca tcatttcgca
    19321 caacctggcg cagcgtaaag ccgcagcggt tgaggcggaa actattgtcg ctcaggaaac
    19381 cagcgaattt atggcgtggc tgcgagcaca aagcgccagc gaaaccattc gcgagtatcg
    19441 cagccaggca gagcaagttc gcgatgagtt aaccgccaaa gcgttagcgg cccttgagca
    19501 gggcggcgac gcgcaagcca ttatgcagga tctggcatgg aaactgacta accgcttgat
    19561 ccatgcgcca acgaaatcac ttcaacaggc cgcccgtgac ggggataacg aacgcctgaa
    19621 tattctgcgc gacagcctcg ggctggagta gcagtacatc attttctttt tttacagggt
    19681 gcatttacgc ctatgaagcc ttctatcgtt gccaaactgg aagccctgca tgaacgccat
    19741 gaagaagttc aggcgttgct gggtgacgcg caaactatcg ccgaccagga acgttttcgc
    19801 gcattatcac gcgaatatgc gcagttaagt gatgtttcgc gctgttttac cgactggcaa
    19861 caggttcagg aagatatcga aaccgcacag atgatgctcg atgatcctga aatgcgtgag
    19921 atggcgcagg atgaactgcg cgaagctaaa gaaaaaagcg agcaactgga acagcaatta
    19981 caggttctgt tactgccaaa agatcctgat gacgaacgta acgccttcct cgaagtccga
    20041 gccggaaccg gcggcgacga agcagcgctg ttcgcaggcg atctgttccg tatgtacagc
    20101 cgttacgccg aagcccgccg ctggcgggta gaaatcatga gcgccagcga gggtgaacat
    20161 ggtggttata aagagatcat cgccaaaatt agcggtgatg gtgtgtatgg tcgtctgaaa
    20221 tttgaatctg gcggtcatcg cgtgcagcgt gttcctgcta cggaatcgca gggtcgtatt
    20281 catacttctg cttgtaccgt tgcggtaatg ccagaactgc ctgacgcaga actgccggac
    20341 atcaacccag cagatttacg cattgatact ttccgctcgt caggggcggg tggtcagcac
    20401 gttaacacca ccgattcggc aattcgtatt actcacttgc cgaccgggat tgttgttgaa
    20461 tgtcaggacg aacgttcaca acataaaaac aaagctaaag cactttctgt actcggtgct
    20521 cgcatccacg ctgctgaaat ggcaaaacgg caacaggccg aagcgtctac ccgtcgtaac
    20581 ctgctgggga gtggcgatcg cagcgaccgt aaccgtactt acaacttccc gcaggggcgc
    20641 gttaccgatc accgcatcaa cctgacgctc taccgcctgg atgaagtgat ggaaggtaag
    20701 ctggatatgc tgattgaacc gattatccag gaacatcagg ccgaccaact ggcggcgttg
    20761 tccgagcagg aataatggaa tatcaacact ggttacgtga agcaataagc caacttcagg
    20821 cgagcgaaag cccgcggcgt gatgctgaaa tcctgctgga gcatgttacc ggcagagggc
    20881 gtacttttat tctcgccttt ggtgaaacgc agctgactga cgaacaatgt cagcaacttg
    20941 atgcgctact gacacgtcgt cgcgatggtg aacccattgc tcatttaacc ggggtgcgag
    21001 aattctggtc gttgccgtta tttgtttcgc cagcgacctt aattccgcgc ccggatacgg
    21061 agtgtctggt ggagcaggca ctggcgcggt tgcctgaaca accttgccgt attctcgatc
    21121 tcgggacggg taccggggcg attgcgcttg cgctggctcg cgagcgcccg gactgcgaaa
    21181 ttaccgctgt agatcgtatg cccgatgctg tcgccctggc acaacgtaat gcccagcatc
    21241 tggcgatcaa aaatatccac attctgcaaa gcgactggtt tagcgcgcta gccgggcagc
    21301 agtttgcgat gattgtcagc aatccgccgt atattgacga gcaggaccct catcttcaac
    21361 aaggcgatgt ccgctttgag ccgctcactg cgctggttgc ggcagacagt ggaatggcag
    21421 acatcgtgca tatcatcgaa cagtcgcgta acgcgctggt atccggcggc tttctgcttc
    21481 tggaacatgg ctggcagcag ggcgaagcgg tgcgacaggc atttatcctc gcggggtatc
    21541 atgacgtcga aacctgccgt gactatggtg ataacgagcg cgtaacgctc ggccgctatt
    21601 atcaatgaca agtttttcta cactgcttag tgttcatctt attagtatcg cgctttctgt
    21661 tgggctatta accttacgtt tctggctacg ttatcagaag catccacagg catttgctcg
    21721 ctggacgcgc attgtgccgc cggttgtcga tacggtgtta ttgttaagcg gcattgcgtt
    21781 gatggctaaa gcgcacatcc tgccattttc cgggcaggca cagtggctga ctgaaaagct
    21841 gtttggagtt atcatttata tcgttttggg ttttattgca ctcgattatc gtcgtatgca
    21901 cagtcagcag gcgcgcatta ttgccttccc gctgacgttg gtggtgctgt acatcatcat
    21961 taaactcgcc accacaaaag taccgttact ggggtaagtc atgagatcgt tagctgattt
    22021 cgaatttaat aaagcgccat tgtgcgaagg catgatcctg gcttgcgaag caatccgccg
    22081 cgattttccc tcgcaagatg tttacgacga actggagcgt ctcgttagtc tggcgaagga
    22141 agaaatcagc cagcttctgc ctttagaaga gcagctggaa aaactgatcg cgctgtttta
    22201 cggcgactgg ggatttaaag cctcacgcgg cgtttatcgt ctttccgatg cattatggct
    22261 ggaccaggtg ttaaagaatc gacagggcag tgcggtatca ttaggtgcgg ttttattatg
    22321 ggtcgcgaat cgtctcgatt tgccgctgct gccggtgatt ttccctacgc agctgatatt
    22381 gcgcattgaa tgtccggatg gcgaaatttg gctgattaat ccttttaacg gtgaatcgtt
    22441 aagcgaacat atgctggacg tatggttaaa gggaaatatc agcccgtcgg cggaactgtt
    22501 ttatgaagac cttgatgaag ctgataacat tgaggtaatc cgcaaattgc tggatacact
    22561 caaagcctcg ttgatggaag aaaatcagat ggagctggcg ttacgcacca gcgaagcttt
    22621 attacaattc aacccggaag atccctatga aattcgcgat cgcgggttga tttatgcgca
    22681 actggattgc gaacacgttg cgttgaacga tttaagttat ttcgttgaac agtgtccgga
    22741 agacccgatc agcgaaatga tccgtgcgca aataaataac atcgcgcata aacatattgt
    22801 gctgcattaa ttaatcgaca ttttactcaa ggttaaggcg atcctatgaa acaaaaagtg
    22861 gttagcattg gcgacatcaa cgtagcaaat gacctgccgt tcgtactgtt tggcggtatg
    22921 aacgtgctgg aatctcgcga tctggcgatg cgcatttgcg agcactacgt aaccgtgacc
    22981 cagaaactgg gtatccctta cgtgttcaaa gcctcttttg acaaagccaa ccgctcctcc
    23041 atccactctt atcgtggacc gggcctggaa gaagggatga aaatcttcca ggagttgaag
    23101 cagacttttg gcgtgaaaat tatcaccgac gttcacgaac caagtcaggc acagcccgtt
    23161 gctgatgtcg tggatgtgat tcagttgccg gcgtttcttg ctcgccagac tgacctggtt
    23221 gaagccatgg cgaaaaccgg tgcggtaatt aacgtcaaga aaccacagtt tgtcagcccg
    23281 ggacagatgg gtaatatcgt tgataaattc aaagaaggcg gcaacgaaaa agtgattctt
    23341 tgcgatcgtg gtgctaactt cggctatgac aacctggttg tcgatatgct gggcttcagc
    23401 attatgaaga aagtgtctgg taactcgccg gtgattttcg acgtgaccca cgcattgcaa
    23461 tgccgcgatc cttttggcgc agcttccggc ggtcgtcgtg ctcaggtggc tgagctggca
    23521 cgagccggta tggcggtagg tctggcgggg ctgtttattg aagcgcatcc ggatccggaa
    23581 catgcgaaat gtgatggtcc atccgcgctg ccgctggcta aactggaacc gttcctcagg
    23641 cagatgaaag cgattgatga tctggtgaaa ggtttcgaag aactggatac cagcaagtaa
    23701 tcttttttgc ttgaaaaata aagtcttagc gttctgcgtt aagacttttt tcatgggtgc
    23761 cggatacaaa aaaggccgca ggctgttacc ctgcggccgg tttcgggcgc atattgccat
    23821 cacggcagcc tgacgcccgt tttcgcctta cttccggtta cgccaccagc cgacaatcgc
    23881 tgcggtaata attcccgcca ggatcggtgc tgccaggtcg tgccagaaaa tcatggcaaa
    23941 ctgcgcgagc gtcatatagc cgccttgttg taatgacaac attttgcggc tattcttgaa
    24001 ttgttgatgg ttcaagatta gcccccgttc tgttgtcagg ttttacctct caacgtgcgg
    24061 gggttttctc tttccagcaa ccaatgccac cagggataaa gcccccgcaa cattgcgcct
    24121 caccggataa cgccggcttg gtgtggatac tacgactcaa ttcatcttca cttcatccct
    24181 gaaatgtttg caatgaagag tgcattccgg tttttcaaca gctgttacag tcgtttcatg
    24241 agtgctctgg atgatgcttc cagctcgggt tgccaatatt tacttgtgga agtgataaag
    24301 acaaaaatgg ccgcaggctg ttacccctgc ggccggtttc gggcgcatat tgccatcacg
    24361 gcagcctgac gcccgttttc accttacttc cggttacgcc accagccgac aatcgctgcg
    24421 gtaataattc ccgccaggat cggtgccgcc aggtcgtgcc agaaagtcat ggcaaactgc
    24481 gcgagcgtca tatagccgcc ttgttgtaat gacaacattt tgcggctatt cttgaagtgt
    24541 ctggtttcaa gattagccct gttgtcaggt tttacctctc aacgtgcggg ggttttctct
    24601 ttccagcaac caatgccacc agggataaaa cccccgcaac attgcgcctc accggataac
    24661 gccggcttgg tgtggatact acgtctcaat tcatcttcac ttcatccctg aaatgtttgc
    24721 aataaagagt acattccggc ttttcaacag ctgttgcagt ggtttcgtgc aggttgtggt
    24781 acaggctcgc aattctgatt aacgacctgt ccaggtagta tgaagaaata caataagcag
    24841 gaatttatat tatctccccc ggtttccgtc atcggaggag atataactgt caggcaaata
    24901 tcgtcatcaa ataggcggca aacagtgcca gatgcgctgc gccattgagc acgttagtac
    24961 gtccggtgga gaaggagata tggcacagca ctaaagaggc caccatcacc accatttctg
    25021 gcgcaccaag tgcaaactgc aattcgttac ccgtcataaa ggcaattagc gtgacgacag
    25081 gtacggtaag cgaaattgtt gctaacactg aaccaaagaa cagattcatc gcgcgctgga
    25141 cctggttgtt caacactgct tttaatgcac ctaaaccttc cggcgacaga atcaacagtg
    25201 ccaccaggaa gccagtaaag gcgacagggg cattcatgct gtcgagcaaa gtctccagcg
    25261 ggctggcgtt cattttggtg actgcaataa cggcaatcag gtggataatc aaccagatag
    25321 catgccacag gctgctatgg gcagacggtt taccgtggtg cgggtcgtcg tcatcactgt
    25381 catcttcgtg ctcgtagaca aacaaacttt gatgcgtttt ggtctggatc agcaaaaata
    25441 caccatacat tgccgcagaa attaatgcta ccagtaacgc ctgaccggtt gaaaaattcg
    25501 ccgcaggcag agccattgga aataccagta cgattatcgc cagggggaac agcgcaatta
    25561 aatactgctt aataccaaac agattcatat attgggtggc aaacttacga ccgcccaaca
    25621 ataatgaaaa gccgaccagc ccaccggtaa caatcataat gatcgaatag agcgtatcac
    25681 gcattagcgt tggcgcggcg tcgccggttg ccattaaagc tgaaatcaaa ctaacttcaa
    25741 gaataaccac tgaaaggcta agaataagcg aaccgtaagg ttctcccagg cgatgggcta
    25801 atacgtccgc atgacggaca acactaaagg cgctacttaa aataccaata agcgcaagaa
    25861 gattgatggc aatgaccact ggtagtgtct ggctgcttcc ccacaggaac agcactacca
    25921 gcgccagaac cgggaaaata agcgaagtct ccttgtggcg ggtttttacc gcctcttgag
    25981 catttgacat tatggttatc cctttgcaga tgaatttatc gaaaatgtaa aaaataggta
    26041 ggaaaaataa cagaaagtgt ctggatatcg gtaacatttt acgaattttt accccgttgt
    26101 cgatttttta ctcattgggg cataaaaata agtattacgt ttaggcaatg tttgttttag
    26161 cgtttcttaa gaacagtctg acatgaaaat tcttatgttt ttgcaagagt agatattgtt
    26221 gaccacactt aatgttcaac tttgtaaaag gagtcaacga tgccgtataa aacgaaaagc
    26281 gacctgccgg aaagcgtaaa gcacgttcta ccgtctcatg cccaggatat ctataaagaa
    26341 gcgttcaaca gcgcatggga tcaatataaa gataaagaag atcggcgtga tgacgccagt
    26401 cgcgaagaaa ccgcgcataa agtggcctgg gctgctgtga agcatgaata tgccaaaggg
    26461 gatgatgata aatggcataa aaaatcgtaa aaccggtagc ttagttaaag ctattcgtgc
    26521 ggtgttgcct tgcaagtggt ccgcggattg catattgtcc cgttagtggt ttcaaaatga
    26581 gcagtaaaaa tgtccggaag acaccaaaaa gttgtcgcag ggaagcatgc agtggcggag
    26641 gtgtaagaag gtgataacgc gtgatttctt gatgaatgcc gattgtaaaa cggcatttgg
    26701 tgccattgaa gaatcactct tatggtcagc agaacaacgg gcggcttcgc tggcggcgac
    26761 gctggcttgt cgacctgatg agagaccggt gtggatcttc ggttatggat cgttgatgtg
    26821 gaatccggca ctggagttta ccgagtcgtg caccggtaca ctggttggat ggcatcgcgc
    26881 gttttgcctg cgcctgaccg ccgggcgcgg gactgcgcac cagccgggac ggatgcttgc
    26941 actaaaagag ggcggacgta ccacaggcgt cgcctatcga ctgccagaag agacgctgga
    27001 gcaggaacta accctgttgt ggaagcgcga gatgattacc ggctgttatc tgccaacctg
    27061 gtgtcagctt gatcttgatg atggacgcac agtaaacgcc attgtgttta ttatggaccc
    27121 gcgacatcca gaatatgaat ctgacactcg cgctcaggtc atcgcgccgt tgattgcggc
    27181 ggcgagcggt ccgctgggaa ccaacgcaca atacctgttt tcactggaac aggagcttat
    27241 caaactggga atgcaggacg atgggctgaa tgatttgctg gtatcggtaa aaaaactgct
    27301 ggcggagaat tatccggatg gtgtgttgcg gccgggattc gcctgagtaa acttccctca
    27361 tcgtggggcg tcagacgccc cctcaaacat taaaatgtga gcactttatc ggctgacagc
    27421 gtccattgcg ccagttccac aagagtaccg atttccaccc catcaatcag aggaagtgta
    27481 ctaatcccgc gcccgtcggt acaggttttg cacaatttca ccggtacatt ctgagcggta
    27541 aggatctcca gcatttgctg tatgttgtag ccttcccctg gtttttgccc gcgcaacccg
    27601 gctgtgaccg catcagacat caggaacaga cgcagatcca gattgctctc ctgctctcgt
    27661 aacgcaatgg ccagccgcaa gctgttaaac aaggattcgc tcccgtaagg tgcgccattg
    27721 gcaacgatca cgattttttg cattatttac tcctgtattc agggaattag acactcatct
    27781 tctatcttac tgcttctgca gcgtctgacc aatcggtcac atttttaagg attttcctga
    27841 aagcgcgaga aaatacgaca aaagttgcca gtaatcgtta ttctttaagg ctatgttttt
    27901 tcatttttta ccggaagtta ccgacgtttt gagccgtttc gttcctcgca ttattccgtt
    27961 ttatttactc ttgctggcgg caggcggtac agctaacgca caatctacct tcgagcaaaa
    28021 agcggcaaat ccctttgata ataacaatga tggtctgccg gatttaggca tggcgcctga
    28081 aaatcatgat ggggaaaaac actttgctga aattgtgaaa gatttcggcg aaaccagtat
    28141 gaatgataac gggctggata ctggcgagca ggcaaaagct ttcgcattgg gaaaagtccg
    28201 cgacgcgctt agtcaacagg ttaatcagca cgtagagtcg tggctatcac cgtggggaaa
    28261 tgccagtgtt gacgtcaaag tggataacga aggtcatttt accggcagtc gtggaagctg
    28321 gtttgtgccg ttacaagata atgatcgtta tctcacctgg agccagcttg gtcttactca
    28381 gcaggatgat gggctggtga gcaatgtggg cgttgggcaa cgctgggcgc gcggcaactg
    28441 gctggtgggt tataacactt tttatgacaa cttgctggat gaaaatcttc agcgagcggg
    28501 ctttggtgcc gaagcgtggg gcgaatattt gcgactatcg gcaaactttt atcagccgtt
    28561 tgctgcatgg catgaacaga cagccacgca ggaacaacgg atggcgcgcg ggtacgacct
    28621 gacagcccgg atgcgcatgc cgttctatca acacctcaat accagtgtca gcgtagaaca
    28681 gtattttggt gatcgtgttg atttgtttaa ctctggtacg ggttatcaca atcccgtcgc
    28741 gttgagtctg ggattaaatt acacccctgt gccattagtc actgtgacgg cccagcataa
    28801 acagggtgaa agtggcgaga atcaaaataa cctcgggctg aatcttaatt accgctttgg
    28861 tgtaccgctc aaaaaacaac tttctgcggg cgaggttgcc gaaagtcagt cgttacgtgg
    28921 tagtcgctat gataatccgc agcgaaataa tctaccgact cttgagtacc gacagcgaaa
    28981 aacgttaacg gtgtttcttg cgacaccgcc gtgggatcta aaacctggcg aaacagtgcc
    29041 gctgaaatta caaatccgca gtcgttacgg tattcggcaa ctgatttggc agggcgatac
    29101 gcagatatta agtttgacgc cgggcgcaca agccaacagc gcggagggct ggacgctgat
    29161 catgcctgac tggcagaacg gggaaggggc gagcaatcac tggcgattgt cggtggtggt
    29221 ggaagataac caggggcagc gtgtctcctc caatgagatc acgctaacgc ttgtcgaacc
    29281 gttcgacgca ttgtcaaacg acgaactgcg ctgggaaccg taatcagaaa atgcgctcct
    29341 gatgcaccca taccgctgct tccacgcgag acttgagctt cattttcttc agcatgtgct
    29401 tgacgtgcac ttttactgtg ctttcggtga tgtccaggcg gcgggcaatc atcttgttcg
    29461 gcaaaccctg ggcaatcagc ttgagaatat cgcgctcgcg tggggttaac tggttaacat
    29521 cgcgctcagt cgtggcacgg ttagcgcgca agctggcggc cagaacaggc gttaatgctt
    29581 cgcttaatac catttcgcca gcagctgcct gatgcaatgc tttcagcaga tcttccggtt
    29641 ccatatcttt taacagatag ccatccgcgc cgcgtttcag tgcggtgacc acatcttctt
    29701 catggttaga gacgctgaat accacaatgc gccctgagag ggacttttcg cgcagtttat
    29761 ccagcgtttc cagaccgttc atgccgggca tattgagatc taacaggatc agatcgggat
    29821 caagagattc cgccagttca ataccctgtt cgccattact cgcttcgcca accacggtga
    29881 tatctggtgc catactgata agctgtttta cgccagttcg cagcatcggg tgatcgtcaa
    29941 tcagcaggat agtagccggt tcctgattac tcatgggtat ctccttggac gtctgtgaaa
    30001 gttttttcgg gaataaaggt gaccaccact tcggtgccac ctgattcacg acggcggacg
    30061 cggcaatcgc ctcgtaaact ttgcgcacgg tcgcgcatta ttatcatgcc gtagtgattg
    30121 ctgcggatgg cattttcagg cacgccgcag ccgttatcct ggacggtcag tttgacctga
    30181 ttatcgtttt gcgccaccgt caccacgacc tcactcgctt gcgaatgttt gagggcgtta
    30241 cttaatgcct cacgggcaat ttgcaacaag tggattgcct gatgcgaagg caccagacga
    30301 ggcggcaatt gataatccag cttcaccggg aagccaaatt tggcgctgta ctcttcgcaa
    30361 ctcgcctcca gcgccggacg taatccaggc tcggtgagct gcaagcggaa tgtggtgagc
    30421 aattcacgca actgcgccca ggatgcattc agttcgttac ggatctgact taacagttcg
    30481 cggctgcttt ctggcagcgc atcgccctgc atctgtaaac aactcacctg catcttcatg
    30541 caagagagag attgggcaat agaatcatgc agttcgcgcg caatggtggc acgctcttcc
    30601 atcacgatca actgttgctg acgttcctga tgccgatcca gcgccagcgt ggcggtgagt
    30661 tgttcaacca gggtatccac cagttgttgt tgatcatggc taagatgacg cccctgcggt
    30721 agggtcgcca gcaaaatacc gtactgcgta tgagagtcag ccagccgcca cttcagggtc
    30781 gtgccgcgat caccaacggg taatacgccg cgcgggcaga gctggcagcc tttatcatca
    30841 caagtcatat ctggctggca ggtaaactcc tgatgattct cttcatcatc agtgtcatac
    30901 acccgcagtt cgatatcacg tagcagggtt aaattctgta agccgttgag tacaggtgac
    30961 aggcgttcac acagcggggc gcgggaatgc aaacggcggt tagcctgcca taaaaaagag
    31021 aggatctgat ttttatgctc cagcccggcg gttttctcct gaacccgctg ctcaagtacg
    31081 gcataacttt cggccagttc tgcagacata ttgttcaacg cagttccaag catcgccatt
    31141 tcgttgcgcc cgctgatgtt tgcgcgttgg gtaaaatcgc gatggctgac ggcactcgcc
    31201 attgccagca gttgccgcca cggttgtagc agtcgcgccc gcaaccagat aatagtgaac
    31261 accagtaaaa gtgccataaa taccgccatt acccgatgga ccagtaccac tgtctcaatg
    31321 cgcatttccg tggtgcggtc aaaaccagat accagctgat caagcccggc aacaaactgg
    31381 ctgacatccg ctgacaccgt ttcgcggttt tgtgcacgca tcagtgcagg gatgagttca
    31441 ttacgccagt aatcttgtaa accctgtaat tgcgccagtt gtccgtctcg ttctgctgct
    31501 cgagtcaact cggcgctaaa tgccgtttgt tccatctctt taattaaggg cttgtctttc
    31561 tcgcttaatg gcactgctgc caacagacgg taactttgca tgcgcagcga tcccgctttg
    31621 ttgatcgcat gggcgctgcc ctgaacgcct tgcaccagcc agccagaaac cgccatccct
    31681 gccagtccaa tagcagtaga aagcaacaca ataagcgcaa cctgattaac cagggtgagc
    31741 ggagagagac aacgtttaag catgtaaacc tcttccttca ggctttaaat gagcaataac
    31801 cttaatgaat gtgacgatac attctggaat ggcagtattc tcggctattg gctgaagtat
    31861 acccataccc ggaaagagtt actccttatt tgccgtgtgg ttagtagctt tacatcggta
    31921 agggtaggga ttttacagca ccgtgaaaaa tctcataatt tttatgaaat cactgtactc
    31981 actatgggta atgataaata tcaatgatag ataaagttat cttatcgttt gatttacatc
    32041 aaattgcctt tagctacaga cactaaggtg gcagacatcg aaacgagtat cagaggtgtc
    32101 tatgagtcac tcatccgccc ccgaaagggc tactggagct gtcattacag attggcgacc
    32161 ggaagatcct gcgttctggc aacaacgcgg tcaacgtatt gccagccgca acctgtggat
    32221 ttccgttccc tgtctgctgc tggcgttttg cgtatggatg ttgttcagcg ctgttgcggt
    32281 gaacttaccg aaagtcggtt ttaattttac gaccgatcag ctatttatgt tgactgcgtt
    32341 gccttcggtt tctggcgcgt tattacgtgt tccatactcc tttatggttc ctatcttcgg
    32401 tggtcgtcgc tggacggcgt tcagcaccgg tattctgatt attccttgtg tctggctggg
    32461 ttttgccgtg caggatacct ccacgcctta tagcgtcttc atcatcatct ctctgctgtg
    32521 cggctttgct ggcgcgaact tcgcatccag tatggcaaac atcagcttct tctttccgaa
    32581 acagaagcag ggtggcgcgc tgggcctgaa tggtggtctg ggcaacatgg gcgttagcgt
    32641 tatgcagttg gttgctccgc tggtggtatc actgtcgatt ttcgcagtat ttggtagcca
    32701 gggcgtcaaa cagccggatg ggactgagct gtatctggcg aatgcgtcct ggatatgggt
    32761 gccgttcctt gccatcttca ccattgcggc gtggtttggc atgaacgatc ttgctacctc
    32821 gaaagcctcc atcaaggagc agttgccggt actcaaacgg ggtcatctgt ggattatgag
    32881 cctgctgtat ctggcaacct tcggttcctt catcggcttc tccgcgggct ttgcgatgct
    32941 gtcaaaaacg cagttcccgg atgttcagat tctgcaatac gctttcttcg ggccgtttat
    33001 tggtgcgctg gcgcgttctg caggtggtgc attatctgac cgtctgggcg gaactcgtgt
    33061 cacgctggtg aactttattc tgatggcgat tttcagcggc ctgctgttcc tgaccttacc
    33121 gactgacggg cagggcggaa gcttcatggc gttcttcgcg gtcttcctgg cgctgttcct
    33181 gacagctggg ctgggtagtg gttccacttt ccagatgatt tccgtgatct tccgtaaact
    33241 gacaatggat cgcgtgaaag cagaaggggg ttctgacgaa cgtgcgatgc gtgaagcggc
    33301 aaccgacacg gcggcggcgt tgggtttcat ctctgcgatt ggcgcgattg gtggcttctt
    33361 tatcccgaaa gcgttcggta gctcgctggc attaacgggt tcgccagtcg gcgcaatgaa
    33421 agtatttttg attttctata tcgcctgcgt agtgattacc tgggcggtat atggtcggca
    33481 ttctaaaaaa taaatcttaa tatttgatta tcctttgcgc ggcatgatgt cgcgcttttt
    33541 ttatgcgtca tttagttaca acatactaat gttatatggt ttatttcgcc ggatttcatt
//
LOCUS       DAZFAB010000041        33153 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZFAB010000041 DAZFAB010000000
VERSION     DAZFAB010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.041"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(99..1466)
                     /gene="rhlE"
                     /locus_tag="ACHJWA_002293"
     CDS             complement(99..1466)
                     /gene="rhlE"
                     /locus_tag="ACHJWA_002293"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFO4308886.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRK
                     PAAAQ"
     gene            1695..2366
                     /gene="cecR"
                     /locus_tag="ACHJWA_002294"
     CDS             1695..2366
                     /gene="cecR"
                     /locus_tag="ACHJWA_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFO4308887.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     YRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            2369..3364
                     /gene="hlyD"
                     /locus_tag="ACHJWA_002295"
     CDS             2369..3364
                     /gene="hlyD"
                     /locus_tag="ACHJWA_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFO4308888.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            3357..5093
                     /gene="ybhF"
                     /locus_tag="ACHJWA_002296"
     CDS             3357..5093
                     /gene="ybhF"
                     /locus_tag="ACHJWA_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO4308889.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            5086..6219
                     /gene="ybhS"
                     /locus_tag="ACHJWA_002297"
     CDS             5086..6219
                     /gene="ybhS"
                     /locus_tag="ACHJWA_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4308890.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            6230..7336
                     /gene="ybhR"
                     /locus_tag="ACHJWA_002298"
     CDS             6230..7336
                     /gene="ybhR"
                     /locus_tag="ACHJWA_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4308891.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(7298..7708)
                     /gene="ybhQ"
                     /locus_tag="ACHJWA_002299"
     CDS             complement(7298..7708)
                     /gene="ybhQ"
                     /locus_tag="ACHJWA_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFO4308892.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            7841..8602
                     /gene="ybhP"
                     /locus_tag="ACHJWA_002300"
     CDS             7841..8602
                     /gene="ybhP"
                     /locus_tag="ACHJWA_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO4308893.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            8599..9840
                     /gene="clsB"
                     /locus_tag="ACHJWA_002301"
     CDS             8599..9840
                     /gene="clsB"
                     /locus_tag="ACHJWA_002301"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFO4308894.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            9840..10796
                     /gene="ybhN"
                     /locus_tag="ACHJWA_002302"
     CDS             9840..10796
                     /gene="ybhN"
                     /locus_tag="ACHJWA_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFO4308895.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHVPAGIGVLEAVFIALLAGEHTSKGT
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(10832..11545)
                     /gene="ybhM"
                     /locus_tag="ACHJWA_002303"
     CDS             complement(10832..11545)
                     /gene="ybhM"
                     /locus_tag="ACHJWA_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFO4308896.1"
                     /translation="MESYLQNSNKLDFQHETRILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLIKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIVVFSTAGAMFLISMLAGLLFNIDPGSHRFMIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWSGIILHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(11753..12457)
                     /gene="ybhL"
                     /locus_tag="ACHJWA_002304"
     CDS             complement(11753..12457)
                     /gene="ybhL"
                     /locus_tag="ACHJWA_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFO4308897.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDASNLRKYSILCALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(12593..13045)
                     /gene="moaE"
                     /locus_tag="ACHJWA_002305"
     CDS             complement(12593..13045)
                     /gene="moaE"
                     /locus_tag="ACHJWA_002305"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFO4308898.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFIGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(13047..13292)
                     /gene="moaD"
                     /locus_tag="ACHJWA_002306"
     CDS             complement(13047..13292)
                     /gene="moaD"
                     /locus_tag="ACHJWA_002306"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFO4308899.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(13285..13770)
                     /gene="moaC"
                     /locus_tag="ACHJWA_002307"
     CDS             complement(13285..13770)
                     /gene="moaC"
                     /locus_tag="ACHJWA_002307"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFO4308900.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEVD
                     D"
     gene            complement(13773..14285)
                     /gene="moaB"
                     /locus_tag="ACHJWA_002308"
     CDS             complement(13773..14285)
                     /gene="moaB"
                     /locus_tag="ACHJWA_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFO4308901.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(14307..15296)
                     /gene="moaA"
                     /locus_tag="ACHJWA_002309"
     CDS             complement(14307..15296)
                     /gene="moaA"
                     /locus_tag="ACHJWA_002309"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFO4308902.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(15284..15428)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            15693..16601
                     /gene="yvcK"
                     /locus_tag="ACHJWA_002310"
     CDS             15693..16601
                     /gene="yvcK"
                     /locus_tag="ACHJWA_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFO4308903.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDAHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHSALEKALQALG"
     gene            complement(16792..18813)
                     /gene="uvrB"
                     /locus_tag="ACHJWA_002311"
     CDS             complement(16792..18813)
                     /gene="uvrB"
                     /locus_tag="ACHJWA_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFO4308904.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(19392..20069)
                     /gene="bioD"
                     /locus_tag="ACHJWA_002312"
     CDS             complement(19392..20069)
                     /gene="bioD"
                     /locus_tag="ACHJWA_002312"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO4308905.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAAGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQRNSSLQLDYATVNPYTFAEPTSPHIISAQEGRSIESSVMSSG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(20062..20817)
                     /gene="bioC"
                     /locus_tag="ACHJWA_002313"
     CDS             complement(20062..20817)
                     /gene="bioC"
                     /locus_tag="ACHJWA_002313"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFO4308906.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRQSADVLLAMLPQRKYP
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHRIQPITLWFDDALSAMRSLKGIGATHLHEGRDQRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIVRE"
     gene            complement(20804..21958)
                     /gene="bioF"
                     /locus_tag="ACHJWA_002314"
     CDS             complement(20804..21958)
                     /gene="bioF"
                     /locus_tag="ACHJWA_002314"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFO4308907.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFVHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     TCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSDEGDARREKLAALITRFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPSGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(21955..22995)
                     /gene="bioB"
                     /locus_tag="ACHJWA_002315"
     CDS             complement(21955..22995)
                     /gene="bioB"
                     /locus_tag="ACHJWA_002315"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFO4308908.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMSYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            23082..24371
                     /gene="bioA"
                     /locus_tag="ACHJWA_002316"
     CDS             23082..24371
                     /gene="bioA"
                     /locus_tag="ACHJWA_002316"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFO4308909.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQHFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            24430..24906
                     /gene="ybhB"
                     /locus_tag="ACHJWA_002317"
     CDS             24430..24906
                     /gene="ybhB"
                     /locus_tag="ACHJWA_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFO4308910.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            25224..25316
                     /locus_tag="ACHJWA_002318"
                     /pseudo
     CDS             25224..25316
                     /locus_tag="ACHJWA_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549435.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(25413..26111)
                     /gene="nleD"
                     /locus_tag="ACHJWA_002319"
     CDS             complement(25413..26111)
                     /gene="nleD"
                     /locus_tag="ACHJWA_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector zinc metalloprotease NleD"
                     /protein_id="HFO4308911.1"
                     /translation="MRPTSLNLVLHQSSRSSSMSDTDIESLVKASSVQWIKNNPQLRF
                     QGTDHNIYQQIEAALDKIGSTETGRVLLNAIESISRLKSETVVIHLNSSRLGVMAHRD
                     IDAENHRGTGSDFHCNLNAVEYPCGEGISVVDFHATIVFHELLHVFHNLNGERLKVES
                     SRAESQKYSPLLLEEARTVGLGAFSEEVLSENKFHEEIGMPRRTSYPHDSALIHDDNT
                     VSLGFQQVRLHPLL"
     gene            complement(26173..26294)
                     /locus_tag="ACHJWA_002320"
                     /pseudo
     CDS             complement(26173..26294)
                     /locus_tag="ACHJWA_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(26342..27223)
                     /gene="nleH1-1"
                     /locus_tag="ACHJWA_002321"
     CDS             complement(26342..27223)
                     /gene="nleH1-1"
                     /locus_tag="ACHJWA_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector kinase
                     NleH1-1"
                     /protein_id="HFO4308912.1"
                     /translation="MLSPYSVNLGCSWNSLTRNLTSPDNRVLSSVRDAAVHSDNGAQV
                     KVGNRTYRVVATDNKFCVTRESHSGCFTNLLHRLGWPKGEISRKIEVMLNASPVSAAM
                     ERGIVHSNRPDLPPVDYAPPELPSVDYNRLSVPGNVIGKGGNAVVYEDAEDATKVLKM
                     FTTSQSNEEVTSEVRCFNQYYGAGSAEKIYGNNGDIIGIRMDKINGESLLNISSLPAQ
                     AEHAIYDMFDRLEQKGILFVDTTETNVLYDRAKNEFNPIDISSYNVSDRSWSESQIMQ
                     SYHGGKQDLISVVLSKI"
     gene            complement(27392..27547)
                     /locus_tag="ACHJWA_002322"
                     /pseudo
     CDS             complement(27392..27547)
                     /locus_tag="ACHJWA_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            27826..27930
                     /locus_tag="ACHJWA_002323"
                     /pseudo
     CDS             27826..27930
                     /locus_tag="ACHJWA_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            complement(28050..29069)
                     /gene="nleC"
                     /locus_tag="ACHJWA_002324"
     CDS             complement(28050..29069)
                     /gene="nleC"
                     /locus_tag="ACHJWA_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector zinc
                     metalloprotease NleC"
                     /protein_id="HFO4308913.1"
                     /translation="MICRVLDINMKIPSLQSNFNFSAPAGYSAPIAPNRAENAYADYV
                     LDIGKRIPLSAADLSNVYESVIRAVHDSRSRLIDQHTVDMIGNTVLDALSRSQTFRDA
                     VSYGIHNEKVHIGCIKYRNEYELNEESSVKIDDIQSLTCNELYEYDVGQEPIFPICEA
                     GENDNEEPYVSFSVAPDTDSYEMPSWQEGLIHEIIHHVTGSSDPSGDSNIELGPTEIL
                     ARRVAQELGWSVPDFKGYAEPEREAHLRLRNLNALRQAAMRHEENERAFFERLGTISD
                     RYEASPDFTEYSAVSNIGYGFIQQHDFPGLAINDNLQDANQIQLYHGAPYIFTFGDVD
                     KHNQQ"
     gene            complement(29103..30083)
                     /gene="nleB2"
                     /locus_tag="ACHJWA_002325"
     CDS             complement(29103..30083)
                     /gene="nleB2"
                     /locus_tag="ACHJWA_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB2"
                     /protein_id="HFO4308914.1"
                     /translation="MLSPIRTTFHNSVNIVQSSPCQTVSFAGKEYELKVIDEKTPILF
                     QWFEPNPERYKKDEVPIVNTKQHPYLDNVTNAARIESDRMIGIFVDGDFSVNQKTAFS
                     KLERDFENVMIIYREDVDFSMYDRKLSDIYHDIICEQRLRTEDKRDEYLLNLLEKELR
                     EISKAQDSLISMYAKKRNHAWFDFFRNLALLKAGEIFRCTYNTKNHGISFGEGCIYLD
                     MDMILTGKLGTIYAPDGISMHVDRRNDSVNIENSAIIVNRSNHPALLEGLSFMHSKVD
                     AHPYYDGLGKGVKKYFNFTPLHNYNHFCDFIEFNHPNIIMNTSQYTCSSW"
     gene            complement(30151..30258)
                     /locus_tag="ACHJWA_002326"
                     /pseudo
     CDS             complement(30151..30258)
                     /locus_tag="ACHJWA_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309586.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(30260..30529)
                     /locus_tag="ACHJWA_002327"
     CDS             complement(30260..30529)
                     /locus_tag="ACHJWA_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO4308915.1"
                     /translation="MNILRKLMQSLCGCGKHDDCENGRSLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
     gene            complement(30531..31847)
                     /locus_tag="ACHJWA_002328"
     CDS             complement(30531..31847)
                     /locus_tag="ACHJWA_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO4308916.1"
                     /translation="MAAVQISGVLKDGAGKPIQNCTIQLKARRNSTTVVVNTVASENP
                     DEAGRYSMDVEYGQYSVTLLVEGFPPSHAGTISVYEDSQPGTLNDFLGAMTEDDARPE
                     ALRRFEQMVEEAARHAEEAKKNAGEAETSARNAGISASKAEASAANADTSAEDASESA
                     RQAAESAASAKKSEEASSSSASEAAQKASESLQSATDAELSKKTAESAAGNAARDATT
                     ATEKARESAESAQSAEQSRIAAEDAVNRIPTVVGPPGPKGEPGPAGPQGPKGDKGERG
                     DTGPAGATGERGPGGDTGPAGPQGPKGDRGERGETGLTGSTGPQGPKGDTGATGPAGP
                     QGPKGETGAAGPVGATGPQGAKGDPGETQIRFRLGPMRIIETNSYGWFPGTDGALITG
                     LTFLDPKDATQVQGMFQHLQVRFGDGPWQDVKGLDEVGSDTGRTGE"
     gene            complement(31907..32506)
                     /locus_tag="ACHJWA_002329"
     CDS             complement(31907..32506)
                     /locus_tag="ACHJWA_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO4308917.1"
                     /translation="MRKVCAAILSAAICLAVSGVPAWASEHQSTLSAGYLHASTDAPG
                     SDDLNGINVKYRYEFTDTLGLITSFSYANAEDEQKTHYSDTRWHEDYVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYFRVTDNKRKTHDVLTGSDDARYSNTSLAW
                     GAGVQFNPTESVAVDVAYEGSGSGDWRTDGFIVGVGYKF"
     gene            complement(32577..>33153)
                     /locus_tag="ACHJWA_002330"
     CDS             complement(32577..>33153)
                     /locus_tag="ACHJWA_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4308918.1"
                     /translation="FSLTSDGKLTAKNADISGSVNANSGTLNNVTVNENCTIKGMLEA
                     TQVRGDFVKAVSKSFPKQAGTWGNTETPNGTVTVTISDDHNFDRQIIIPPIIFNGIAY
                     SDPGSGNNPGGTRYTGYGFEVRKNGVLIASRETKGAIPGSYSAVIDMPSGRGSVTLEF
                     KVFHKGNQRAGNITDCTVIVTKKAASGISIR"
BASE COUNT         7912 a         8818 c         8116 g         8307 t
ORIGIN      
        1 tttattaaaa caataaaatc agacatgtca tgcatgtctg accgtgttac ctgaataatg
       61 ggcaaaagcc tgtgatactc ttttgccctt acatggtatt actgcgcagc ggcaggttta
      121 cgcggacggc gacggcgttg ttgttcgcct gccggtttgg catcgccaag acgacgagat
      181 ggtttttctg caggcttcgc gctagctgat ttcgcaccac cttctccacg gcgtggttgc
      241 tgttgaccgc gaccaccacc accttgccca cgaccgccgc cgccacgttg ctggcgaccg
      301 ttctggatcg gttcggcttt gattgacggg tccggctcat aacccggaat cgcaatgcgc
      361 gggatctctt ttttcagcag tttttcgata tcacgcagca gtttgtgttc atcaacacac
      421 accagcgaca atgcttcgcc agttgccgcc gcacggccag tacgcccgat acggtggaca
      481 taatcttccg gtacgtttgg cagttcatag ttgacgacgt gcggcaactc ttcgatatcc
      541 agaccgcgcg cagcgatgtc agttgccacc agtacacgaa tatcgcccga tttaaaatcg
      601 gccagcgcac gggtacgcgc accttgcgat ttattaccgt ggatcgccgc actacggatg
      661 ccatctttat taagctgttc agcgagatgg ttagcgccat gtttggtacg ggtaaacacc
      721 agcacctgct gccagttccc tttcccaatc atgtgcgaca gcaattcgcg cttgcgtttc
      781 ttatcaacaa agtgaacatg ctgggtcacc tgatcagacg cggtattgcg gcgtgccact
      841 tctatttcca gcgggttgtg caacagtttt tccgccaggg ctttaatatc gtcagagaag
      901 gtcgcggaga acaacaggtt ctggcgcttc gcaggtagtt ttgttaacac gcgacggata
      961 tcgtggataa agcccatgtc gagcatgcgg tcagcttcat cgaggacgag gatttcaacc
     1021 tgatccagct tcactgcatt ctgatgttcc aggtccagca aacgtcccgg cgttgccacc
     1081 agcacatcaa cgccgccgcg caacttcatc atttgtgggt taatgctgac accgccaaac
     1141 accaccagcg aacgaatgtt caggtatttg ctgtaatcac ggacgttttc gccaatctgc
     1201 gccgccagtt cacgggtcgg ggtaagaatg agcgcacgta ccggacgacg ccctttggcg
     1261 tgcggctggc gagtgatcag gtgttgcaac agcggcagcg taaagcccgc tgttttgccg
     1321 gtgccggtct gggcgctagc catcaggtcg cggccttcca gcaccgcagg gatcgcctgc
     1381 tgctgaatag gggtgggttc acggtaaccc tgctcggcaa cggcgcgcag gatatcaggg
     1441 cttaaaccca aagaatcgaa agacataact actccgaacc gccccgaccg ttaccggtgt
     1501 agttttcagg gagatactga aaaggaaaat gacaaaaacc acaatgtcat gagggagcgg
     1561 agtgtagcag tttttgtgac gcagcgcata aattatccct gtgactggcg tagctaaaat
     1621 tcttagccat actggacaac tcccatatgg agtcataatc ttaatcaatc atttgattaa
     1681 gaatggactg tgcgatgaat aatcctgcca tgacaatcaa gggtgaacag gcgaaaaaac
     1741 agctgattgc tgccgcactg gcgcagtttg gtgaatatgg aatgaacgcc accacccgcg
     1801 agatagccgc ccaggccggg cagaatatcg ctgccatcac ctactacttc ggttcgaaag
     1861 aagatttgta cctcgcctgc gcccagtgga ttgccgattt tattggcgag cagttccgtc
     1921 cgcatgccga ggaagccgaa cgcttgttcg cccaaccaca gcctgatcgg gctgccatcc
     1981 gtgaactgat ccttcgcgcc tacaggaaca tgattaagct gctcacccag gatgacaccg
     2041 tcaacctcag caagtttatc tcccgtgagc agctctctcc cacggcagcc taccacctgg
     2101 tgcacgaaca ggtgattagt ccgctacaca gccacctgac acgcctgatt gccgcctgga
     2161 ccggctgcga cgccaatgac acccgcatga tccttcatac ccatgcgttg attggtgaga
     2221 ttctggcgtt ccgtcttggc aaagaaacaa ttctgttacg taccggctgg accgcgttcg
     2281 atgaagaaaa gaccgaactg atcaaccaga cggtgacctg tcatatcgac ctgattttgc
     2341 aaggattatc gcaaaggagt ttgtagtgat gaaaaaacct gtcgtgatcg gactggcggt
     2401 agtggtactt gccgccgtgg ttgccggagg ctactggtgg tatcaaagcc gccaggataa
     2461 cggcctgacg ctgtatggca acgtggatat tcgtacggtg aatcttagtt tccgtgttgg
     2521 ggggcgcgtt gaatcgctgg cggtggacga aggtgatgct atcaaagcgg gccaggtgct
     2581 gggcgaactg gatcacaagc cgtatgagat tgccctgatg caggcgaaag cgggcgtctc
     2641 ggtggcacag gcgcagtatg acctgatgct tgccgggtat cgcgatgaag aaatcgctca
     2701 ggccgccgca gcggtgaaac aggcgcaagc cgcctatgac tatgcgcaga acttctataa
     2761 ccgccagcaa ggactgtgga aaagccgcac catttcggca aatgacctgg aaaatgcccg
     2821 ctcctcgcgc gaccaggcac aggcaacgct gaaatcagca caggataaat tgcgtcagta
     2881 ccgttccggt aaccgtgaac aggacatcgc tcaggcgaaa gccagcctcg aacaggcgca
     2941 ggcgcaactg gcgcaggcgg agctgaattt acaggactca acgttgatag caccgtctga
     3001 tggcacgctg ttaacgcgcg cggtggagcc aggcacggtt ctcaatgaag gtggcacggt
     3061 gtttaccgtt tcactaacgc gtccggtgtg ggtgcgtgct tatgttgatg aacggaatct
     3121 tgaccaggcc cagccggggc gcaaagtgct gctttatacc gatggtcgcc cggacaagcc
     3181 ctatcacggg cagattggtt tcgtttcgcc gactgctgaa tttaccccga aaaccgtcga
     3241 aacaccggat ctgcgtaccg acctcgtcta tcgcctgcgt attgtggtga ccgacgccga
     3301 tgatgcgtta cgccagggaa tgccagtgac ggtacaattc ggtgacgagg caggacatga
     3361 atgatgccgt tatcacgctg aacggcctgg aaaaacgctt tccgggcatg gacaagcccg
     3421 ccgtcgcgcc gctcgattgc accattcatg ccggttatgt gacggggctg gtagggccag
     3481 acggtgcggg taaaaccacg ctgatgcgga tgctggcggg attactgaaa cccgacagcg
     3541 gcaatgccac ggtgattggc tttgatccga tcaaaaacga cggcgcgctg cacgccgtgc
     3601 tcggctatat gccgcagaaa ttcggcctgt atgaagatct gacggtgatg gagaacctca
     3661 atctgtacgc ggatttgcgc agcgtcaccg gcgaggcacg gaagcagact tttgctcgcc
     3721 tgctggagtt tacgtctctt ggcccgttta ccggacgcct ggcgggcaag ctctccggtg
     3781 ggatgaaaca aaaactcggc ctggcctgta ccctggtggg cgaaccgaaa gtgttgctgc
     3841 tcgatgaacc cggcgtcggc gttgacccta tctcacggcg cgaactgtgg cagatggtgc
     3901 atgagctggc gggcgaaggg atgttaatcc tctggagtac ctcgtatctc gacgaagccg
     3961 agcagtgccg tgacgtgtta ctgatgaacg aaggcgagtt gctgtatcag ggagaaccaa
     4021 aagccctgac acaaaccatg gccggacgca gctttctgat gaccagtcca cacgagggca
     4081 accgcaaact gttgcaacgc gccttgaaac tgccgcaggt cagcgacggc atgattcagg
     4141 ggaaatcggt acgtctgatc ctcaaaaaag aggccacacc agacgatatt cgccatgccg
     4201 acgggatgcc ggaaatcaac atcaacgaaa ctacgccgcg ttttgaagat gcgtttattg
     4261 atttgctggg cggtgccgga acctcagaat cgccgctggg tgcaatattg catacggtag
     4321 aaggcacacc cggcgagacg gtgatcgaag cgaaagaact gaccaagaaa tttggtgatt
     4381 ttgccgccac cgatcacgtc aactttgccg ttaaacgcgg ggagattttt ggtttgctgg
     4441 ggccaaacgg cgcgggcaaa tcgaccacct ttaagatgat gtgcggtttg ctggtgccga
     4501 cttccggcca ggcgctggtg ctggggatgg atctgaaaga gagttccggt aaagcgcgcc
     4561 agcatctcgg ctatatggcg caaaaatttt cgctctacgg caacctgacg gtcgaacaga
     4621 atttacgctt tttctctggt gtgtatggct tacgcggtcg ggcgcagaac gaaaaaatct
     4681 cccgtatgag cgaggcattt ggcctgaaaa gtattgcctc acacgccacc gatgaactgc
     4741 cattaggttt taaacagcgg ctggcgctgg cctgttcgct gatgcatgaa ccggatattc
     4801 tgtttctcga cgaaccgact tccggcgttg accccctcac ccgccgtgaa ttctggctgc
     4861 acatcaacag catggtagag aaaggcgtca cggtgatggt caccacccac tttatggatg
     4921 aagcggaata ttgcgaccgc atcggcctgg tgtaccgcgg gaaattaatc gccagcggca
     4981 cgccggacga tttgaaagca cagtcggcta acgatgagca acccgatccc accatggagc
     5041 aagcctttat tcagttgatc cacgactggg ataaggagca tagcaatgag taacccgatc
     5101 ctttcctggc gtcgcgtgcg ggcgctgtgc gtcaaagaga cgcggcagat cgttcgcgat
     5161 ccgagtagct ggctgattgc ggtagtgatc ccgctgttgc tgctgtttat ttttggttat
     5221 ggcattaacc tcgactccag caagctgcgg gtcgggattt tactggaaca gcgtagcgaa
     5281 gcagcgctgg atttcaccca caccatgacc ggttcgccct acatcgacgc caccatcagc
     5341 gataaccgtc aggaactgat cgccaaaatg caggcgggga aaattcgcgg tctggtggtt
     5401 attcccgtgg atttcgccga acagatggag cgcgccaacg ctaccgcacc gattcaggtg
     5461 atcaccgacg gcagtgagcc gaataccgct aactttgtgc agggatatgt cgaagggatc
     5521 tggcagatct ggcaaatgca gcgggcggag gataacgggc agacctttga gccgcttatt
     5581 gatgtacaaa cccgctactg gtttaacccg gcggcgatta gccagcactt tattatcccc
     5641 ggtgcggtga ccatcatcat gacggtcatc ggcgcgattc tcacctcgct ggtggtggcg
     5701 cgagaatggg aacgcggcac tatggaggct ctgctctcta cggagattac ccgcacggaa
     5761 ctgctgctgt gtaagctgat cccttattac tttctcggga tgctggcgat gttgctgtgt
     5821 atgctggtgt cagtgtttat tctcggcgtg ccgtatcgcg gttcgctgct gattctgttt
     5881 tttatctcca gcctgttttt actcagtacc ctggggatgg ggctgctgat ttccacgatt
     5941 acccgcaacc agttcaatgc cgctcaggtc gccctgaacg ccgcgttcct gccgtcgatt
     6001 atgctttccg gctttatttt tcagatcgac agtatgcccg cggtgatccg cgcggtgacg
     6061 tacattattc ccgcacgtta tttcgtcagc accctgcaaa gcctgttcct cgccgggaat
     6121 attccagtgg tgctggtggt gaacgtactg tttttgatcg cttcggcggt gatgtttatc
     6181 ggcctgacgt ggctgaaaac caaacgtcgg ctggattagg gagaagagca tgtttcatcg
     6241 cttatggacg ttaatccgca aagagttgca gtcgttgctg cgcgaaccac aaacccgcgc
     6301 gattctgatt ttaccggtgc tgattcaggt gattctgttc ccgttcgccg ccacgctgga
     6361 agtgaccaac gccaccatcg ccatctacga tgaagataac ggcgagcatt cggtggagct
     6421 gacccaacgt tttgcccgcg ccagcgcctt tacacatgtg ctgctgctga aaagcccaca
     6481 ggagatccgc ccgaccatcg acacacaaaa ggcgttacta ctggtgcgtt tcccggctga
     6541 cttctcgcgc aaactggata ccttccagac cgcgccgttg cagttgatcc tcgacgggcg
     6601 taactccaac agtgcgcaaa ttgccgccaa ctacctgcaa cagatcgtca aaaattatca
     6661 gcaggagctg ctggaaggaa aaccgaaacc taacaacagc gagctggtgg tacgcaactg
     6721 gtataacccg aatctcgact acaaatggtt tgtggtgccg tcgctgatcg ccatgatcac
     6781 cactattggc gtaatgatcg tcacttcact ttccgtcgcc cgcgaacgtg aacaaggtac
     6841 gctcgatcag ctactggttt cgccgctcac cacctggcag atcttcatcg gcaaagccgt
     6901 accggcgtta attgtcgcca ccttccaggc caccattgtg ctggcgattg gtatctgggc
     6961 gtatcaaatc cccttcgccg gatcgctggc gctgttctac tttacgatgg tgatttatgg
     7021 tttatcgctg gtgggattcg gtctgttgat ttcatcactc tgttcaacac aacagcaggc
     7081 gtttatcggc gtgtttgtct ttatgatgcc cgccattctc ctttccggtt acgtttcgcc
     7141 ggtggaaaac atgccagtat ggctgcaaaa cctgacgtgg attaatccta ttcgccactt
     7201 tacggacatt accaagcaga tttatttgaa ggatgcgagt ctggatattg tgtggaatag
     7261 tttgtggccg ctactggtga taacggccac gacagggtca gcggcgtacg cgatgtttag
     7321 acgtaaggtg atgtaacttc ttatctttcg ccagcaaaga cactaccgcc gggccggcaa
     7381 ggattgccag ccctgcaatt gccagcaaaa agttgttttc cagtacgcgg gacaacagcc
     7441 acagcacaat cagcgctgcg ccaaaatacc aggtcgtggt cagctcttcc agcacgtctg
     7501 ccacctcacg ccagcgttgc tcggggtggc gctgaaacac cagcatcatc actaccgtga
     7561 cacaatacag tgcgcataat cccacgccga cgtgaaccag agtcttactc atccagccca
     7621 ccaccagcag cgccactacc agtaaatgca acataatctg ccagcaactt agacccgttg
     7681 cgacacgaac acgttgttgc cacttcatgg cttctctcct gaagaattta tctctactta
     7741 ctcagagtac cgaactttac ggaaaattcc gtaagaccgc acctttttgt gacgccgact
     7801 acactatttc aatgagataa gaggcaaaga caggaaaatt atgcccgatc aaacacaaca
     7861 attttcgttc aaggtgctca ccatcaatat tcacaaaggc tttaccgcgt ttaaccgacg
     7921 cttcattttg ccggaacttc gcgatgccgt gcgtaccgtc agcgccgata ttgtttgcct
     7981 gcaggaagtg atgggcgcgc acgaagttca tccgctgcat gtggaaaact ggcccgatac
     8041 ctcgcactac gagtttctcg ccgacactat gtggagcgat tttgcctacg gtcgcaatgc
     8101 cgtatacccg gaagggcatc acggcaacgc cgtactgtcg cgttatccca ttgaacatta
     8161 tgagaatcgc gatgtttcgg tcgatggtgc ggaaaagcgc ggcgtgctct actgccgcat
     8221 tgtgccgccg atgaccggaa aagcgattca tgtgatgtgc gtacatctgg gcctgcgtga
     8281 ggcgcaccgt caggcgcagc ttgcgatgct cgccgaatgg gtgaatgagc taccggacgg
     8341 cgaaccggta ttggtggcgg gtgatttcaa tgactggcgg caaaaagcta atcatccgtt
     8401 aaaagtgcag gccggactgg atgagatttt tacccgcgcc cacggacgcc cggcgcgcac
     8461 gtttccggtg caatttcctc tactacgact ggacaggatc tacgtcaaaa atgccagcgc
     8521 cagcgcgcca accgcgttgc cgctgcggac atggcgacac ctttctgatc atgccccttt
     8581 aagtgcggag attcatttat gaaatgtagc tggcgcgaag gcaataagat ccagttgctg
     8641 gaaaacggcg agcaatatta tcccgcggtg tttaaggcga ttggcgaggc acaagaacgc
     8701 atcattcttg aaacgtttat ctggtttgag gatgacgtcg gcaaacaact gcatgcggca
     8761 ctactggcag cagcgcaacg cggggttaaa gcggaagtct tgctggatgg ctacggttcg
     8821 ccggatctca gcgatgagtt tgtcaatgaa ctgacggcag ctggcgtagt gttccgctac
     8881 tacgatcccc gccctcgcct ttttggtatg cgcaccaatg tgtttcgccg gatgcatcgc
     8941 aaaattgtgg taatcgacgc gcgtatcgcc tttattggcg ggctgaatta ctccgccgag
     9001 catatgtcca gctacggtcc agaggctaaa caggattacg cggtacgcct tgaagggccg
     9061 attgtcgaag atattctcca gtttgagctg gaaaacctgc ctggacagag cgcggcacga
     9121 cgctggtggc gacgtcatca caaagcggaa gagaaccgcc agccgggaga agcgcaggta
     9181 ttgctggtct ggcgcgataa cgaagaacat cgcgatgata ttgaacgtca ctatctgaaa
     9241 atgctcactc aggcgcggcg agaagtcatt atcgccaacg cctacttctt ccccggctat
     9301 cgatttttac acgccttgcg taaagcggca cggcgcgggg tgcggatcaa actgatcatt
     9361 cagggcgaac cggatatgcc gattgtcaga gtcggtgcgc gcttgctgta taactatctg
     9421 gttaaaggcg gcgttcaggt gtttgagtac cgccgccgcc cgctccacgg caaagtggca
     9481 ttgatggacg atcactgggc gacagtaggg tccagtaatc tcgatccgct cagtttgtca
     9541 ctgaatctcg aagcaaatgt catcatccac gatcgtcatt ttaaccagac gctgcgcgat
     9601 aatctgaacg gcattattgc cgcagattgt cagcaggtcg atgagaccat gctgccgaaa
     9661 cgcacctggt ggaacctaac caaaagcgtg ctggcgttcc actttttacg ccacttcccg
     9721 gcgctggttg gctggcttcc ggcacacacg ccacgtctgg cgcaggttga tcctcccgca
     9781 caaccgacaa tggaaacgca ggatcgggta gaaactgaaa acacgggggt aaaaccctga
     9841 tgagtaaatc acacccgcgc tggcgcttag caaagaagat cctcacctgg ctgtttttta
     9901 tcgcggtgat tgtgttgctg gtggtctacg ccaaaaaagt ggactgggaa gaggtctgga
     9961 aggtcatccg cgactacaat cgcgttgcgc tgcttagtgc ggtcgggctg gtggtcgtca
    10021 gctatctgat ttacggctgc tatgacctgc tcgcccgctt ttactgcggt cacaaactgg
    10081 cgaagcgcca ggtgatgctg gtgtcgttta tctgctacgc cttcaacctg acgctcagta
    10141 cctgggtcgg cggcattggt atgcgctatc gtttgtactc ccgtctggga ttgccgggca
    10201 gcactattac gcggattttc tcgctcagta ttaccaccaa ctggctgggc tatattttac
    10261 tggcagggat tatctttacc gcaggcgtgg tggagttgcc ggaccactgg tatgtcgatc
    10321 aaactacgct gcgtattctc ggcattggct tactgatgat tatcgcggtt tatttgtggt
    10381 tttgcgcttt cgcgaagcac cgccatatga ccatcaaagg gcaaaagctg gtgctgcctt
    10441 cgtggaaatt cgccctcgcc cagatgctga tttccagcgt taactggatg gtcatggggg
    10501 cgattatctg gctgttactt ggtcaaagcg tgaactattt ctttgtactg ggcgtgttgc
    10561 tggttagcag tattgccggc gtcatcgtgc atgttccggc ggggattggt gtgctggaag
    10621 cggtgtttat cgcgctactg gctggggagc atacatccaa aggcacaatt atcgccgccc
    10681 tactcgctta ccgtgtgctg tattacttta tcccgctgct gctggcgctg atctgttacc
    10741 tgttgctgga aagccaggcg aagaagctac gggcgaaaaa tgaagcggcg atgtgaagtt
    10801 tagttaagtt ctttagtatg tgcatttacg actaatgaaa aaaacgcgta tgcctttgcc
    10861 agacaagcgt tatagctatc gcggcaagta tcccaaagaa gccgataaaa tagtaataga
    10921 gtgttaatgc acctgtaaaa gcgcaacgaa ccggactcca caactcttca ctatggcatt
    10981 tccccgccaa ttcaaggagc ttatttcgtc catgcaagat aatgcctgac cataacacaa
    11041 tcattaagca acttattacc caaatgggcc gttcactcat taatgcggca ttcacgatga
    11101 ttaccagggc caaccctgtc aacgtcatca taatcataaa acggtgagaa ccaggatcaa
    11161 tattaaataa taaaccggcc agcatactga ttaaaaacat cgcgcccgca gtgctgaaca
    11221 cgacgataat ggcatataca ggaactaaac ttgcaacgac aatcgccacc aaagagagta
    11281 aaaacagata tattacgcct gcgatggcgg catttatctt gttgatatcc ttaattaagc
    11341 catacataca aagtaataat aaccctatgg cgacatacat tggtggtgaa tcatatttgg
    11401 ttgccgtaat ttcgagatac ttagcccccc aggccagccc tgcggttgcc accaaaccta
    11461 aagcggtaat gagccatata ccgtttaata tcctggtctc gtgctgaaaa tctaatttat
    11521 tgctgttttg caagtatgat tccattcttt tagttgtcct tatagaaaaa tattaatatt
    11581 catcatgaat aaatttaaac ccacgcatac aaaatacttt tgtatttatg gatacggaaa
    11641 actcttccgc tcggccataa ggcgtatttt atacctggta cttgttaagt gtcgttttta
    11701 gtaagaaaaa aaatcccgac acatggccgg gatattgatg gtgaaaaaag aattaacgac
    11761 gattgccaaa aattcgcagc aacatcagga acaggttgat gaagtccaga tacaaggtta
    11821 atgcgcaaag aatggaatat ttgcgcaggt tcgacgcgtc gcgggtatct atctgctcac
    11881 ccatattttt cagtttctgg gtgtcatacg ccgtcaatcc gacaaagaca atcacgccga
    11941 tgtaggtgac tgcccacatc aacgcttcgc ttttcagcca gaagttgacc agcgatgcca
    12001 acacaatgcc gattaacgcc ataaacagca tattgccaaa gccacttaaa tcgcgcttcg
    12061 tggtgtaacc gtacaggctc attgcgccga acatcccggc agtaacgacg aaggtactgg
    12121 cgatagaagc agcggtataa acaatgaaaa tactggaaag cgtaagaccc gtcagcgccg
    12181 aataaagcat aaagagcatc gtcgttacac ctgcgctcag cttttgaatc atcgctgata
    12241 acacaataac caatgctaat tgcgcgatga tcagaccgat taaaaagaca cggttagtga
    12301 acaacagctc catcacggcc gcggaattag ccgcatacca ggcaacaaat gcggtcagca
    12361 ataagccaac ggtcatccag ccatagactt gcgccatata agtctgcaag ccagcccggg
    12421 gttgtacaat tgaatcagaa cgtgggaatc tgtccatgac gatctcctga agatataagg
    12481 aatatcttaa ggatactgca aaatgatgag gctgtgcatc gacgcagcgt aaacgcatgt
    12541 actgagcggt gaaattgccg gacgcagcgg tgccttatcc ggctaacaaa aactaccagc
    12601 gttttgccgc ctgctgatcg ctctcccgag cttcgaccca gcggtcgccc tccggcgtgg
    12661 cttcgcgctt ccagaacggt gcgcgggttt tgagataatc cataataaac tgcccggctt
    12721 caaacgcact gctgcgatgc gcactggtga caccgataaa aacgatttca tcgcccggcc
    12781 ataattcccc gatgcggtga atcaccgtga cgcgccccag cggccagcgg ttacgcgctt
    12841 catcgacaat ttctgccagt gctttttcag tcatccccgg atagtgttcg agggttaatg
    12901 ctttgacgct gtcgcccagg ttatggttgc gcaccttacc ggtaaaggtg actaccgcac
    12961 cgtcttcgtc acgctccgcc agccacgggt actcttctcc tacgctgaac ggctgcggac
    13021 caacaacaat tttggtttct gccatcttaa cctccggtta ccggcgggaa gaaagctact
    13081 tcgtcgccgt cagtcagcgg atggtcaaaa ctcaccagcg tctggttgac ggcagccagt
    13141 aatttgccat cttccagcgc cagcgcccag cgatcgctct gcgcagccag gtgctggcgt
    13201 aacgcttcaa cggttgggaa atccgcagcc acttcggttg catctgttcc taccaactcg
    13261 cgcacctggg cgaaaaaaag aactttaatc atccacttcc accttaaagt cacccgactt
    13321 gccgccgctc ttcgccagca aacgtaccgg accaatcacc atatcttttt gcaccgcttt
    13381 gcacatgtca taaatggtca gcgccgccac ggaggccgcg gttaacgctt ccatttcgac
    13441 accggttttc ccggtcaggc ggcataaggt ttctatacgc acccgattgt gctccggctc
    13501 ggcctgtaaa ttgacttcaa ctttgctgag catcagcgga tgacacagcg ggatcagatc
    13561 ccaggtgcgt tttgccgcct gaataccggc aatacgcgca gtggcaaata cgtcgccttt
    13621 gtggtggcga ccatcaataa tcatcgccag cgtctcgctg cgcatggtga caaaggcttc
    13681 cgcccgcgct tcacgcacgg tttccgcttt ggcggagaca tccaccatgt gcgcttcgcc
    13741 agcggcgttg atatgggtca gttgcgacat acttatttct tcaaatgtgg atggaaatta
    13801 cacggacgcg tacgggcatc cagctgcggc gcgatgatat tttcccatgc ggtacggcac
    13861 gctttggtcg aacccggcat ggcgaaaatc agcgttttgt tagcgacgcc cgctaccgca
    13921 cgagattgca acgtggaagt gccaatctct tcaaacgaca acatacggaa cacttcacca
    13981 aaaccttcaa cttcacggtc gaacaacggc agcaatgctt cgggagcctg atcaccttcc
    14041 gtcaggccag tcccccccgt aatcaacacc acttgtacat cgtcgctggc gatccacgca
    14101 gatacctgag cgcgaatagc gtagcggttt tctttcacaa tggctttatc gacaacgtga
    14161 tggcccgctt cttgcgccga atcgcgcaaa tagtgaccgg aggtatcgtc ttcttcaccg
    14221 cgacgattag aaaccgtaag aatagcaata cgggtcggga taaattcagt gcttacctga
    14281 ctcatctgat ctctcctttt gacgttttag ccgccaatgt acgataagtt ttgcgtaata
    14341 ccggtgttgt tttgatgcag gaaatgggtc tgtttcttct cccgcagcgc cgctgaaata
    14401 cgcgcttcca gcgcctgttg ctgggtatcg tcttccagca gatcgcgcag gttaacgccg
    14461 ccttcaccaa acaggcagag atggagttta ccaatggagg aaacgcgcag acggttgcaa
    14521 gtggcgcaga agtctttttc atacggcatg ataaggccaa tctctccggc gtaatccgga
    14581 tgacaaaaga cttgcgcggg accgtcgctg cgttgacgta attggtggat ccagccgcga
    14641 cgcagtagct cgtcacgcag aacctgacca gagatgtgat gcttacggaa aagctcgctg
    14701 ccctcgcccg tttccatcag ttcgatgaaa cgcagctgga tagggcgatg ctggatccag
    14761 ttcagaaagg tgtcgagctg gtgatgattc acatcacgca tcagcacggt attgactttg
    14821 accttctcaa aaccggcctc aaatgcagca tcgatccctg ccatcacctg attgaattta
    14881 tcctggccgg taatggcatg aaactggcgg gcgtccagac tgtcgacgct gacgttaatg
    14941 ccagtaagtc ccgcatcgcg ccagttcgcc acatcgcgtt ccagacggta accattggtg
    15001 gtgaccgcta tctggcgaat agcgtcgttt tctcgcacag cggcgatgat atcggtaaag
    15061 tcgcggcgta aagacggctc accgcctgtc aggcgcactt tttcggtgcc cagactggcg
    15121 aaggcgcgcg taacccggcg aatttcatcg acggtcagaa agcctttatt ggtgacgccg
    15181 ctcggtttgt agccatccgg caggcagtag gtgcaacgaa agttacacac atcggtaatc
    15241 gacaggcgca agtagtaaaa cttacgcgca aatgcatcag tcagttgtga agccatgtac
    15301 acctttccag atacgggagg cgaagtcatt tcttccttcg ccctggtggc aatctttacg
    15361 agcattgtca cggccaaagc accatatcag ttgacccagg tgcagaggct agagtgttta
    15421 gtggttaagc cgatactagc gtgtaaatga gatttttacc atccacactt tcgctatgta
    15481 atcatgtata tagcgtcctg atcgtgctat ttcgctacag aataagggaa aatggcggtt
    15541 ttactttgat atacatcatc agggaggcat agcgtgcatc gtcctttaga ggtagaagaa
    15601 aatagtttgt atcctcaata ttggcaggtt aattgctgtt tccccgcaat ttgcgctact
    15661 gtagcgcgga taatctgact ccaggaaact atatgcgcaa tcgtacgctg gctgatcttg
    15721 atcgtgtcgt tgctctcggc ggagggcatg gactgggacg cgttctctca tcactttcgt
    15781 ctttgggttc tcgtttaacg ggtatcgtca ccaccacgga taatggtggc tcgacggggc
    15841 gtattcgccg ttcagaaggc ggcattgcct ggggcgatat gcgcaactgc cttaaccagc
    15901 tgataactga acccagcgtc gcttcagcaa tgtttgaata tcgtttcggc ggtaatggcg
    15961 aactttccgg gcacaacctt ggaaacctga tgttgaaggc actggatcac cttagcgtgc
    16021 gacctcttga agcgatcaat ctgattcgaa atctgctgaa agtggacgca catttgattc
    16081 cgatgtcgga gcatcctgtt gatctgatgg cgattgacga tcaggggcat gaagtttacg
    16141 gcgaggtcaa tatcgaccag ttaactacgc cgattcaaga gttattgtta acgcctaatg
    16201 tacccgcaac gcgtgaggcg gttcacgcta tcaatgaagc ggatctcatc attattgggc
    16261 ctggcagttt ttacaccagc ctgatgccaa ttctgctgct gaaggaaatc gcgcaggcat
    16321 tacgccgcac gccagcgccg atggtctata tcggcaatct ggggcgtgag ttgagtttac
    16381 ctgcggccaa tttgaagctg gaaagcaagc tggcaattat ggagcagtat gttggtaaaa
    16441 aggtcattga tgcggtcatc gtcgggccaa aagtggatgt ctcggcggtg aaagagcgga
    16501 ttgtgattca ggaggtgctg gaggccagcg atattccgta tcgtcatgac cgccagttgt
    16561 tacatagcgc actggaaaag gcgttacagg ctttaggtta acggaaatcc gaaggaaaat
    16621 tccggcttcc taatgaagac aaagtgcgcg ttgtttatga cggatgcggc gtgaacgcct
    16681 tatccggcct acaaaccgcg ctaattcaat gtattgcaga atcatgtagg cctgataagc
    16741 gtagcacatc aggcaatttt gcttttgtca tctgtcttct tcgttatcct gtcacgaagc
    16801 cgcgataaac aattcacgca gctgatgcaa ctggtcacga atttgtgccg cttcttcgaa
    16861 ctccagattt tgcgcgtgtt gcatcattaa cccttccagc tcatggattt tctgttgcaa
    16921 cgctttaggc gacatatcca tcggcacatt atccggctca acaatcggtc gcgattttcc
    16981 tctgcccttc gctttggttt tagcaatgtt ctgccccaac gccaggatat cgaccacttt
    17041 cttgttcaag ccttgcggcg taatgccgtg ttcctcgttg tacttctgct gtttctcgcg
    17101 gcgacgttcg gtttcgccaa tcgctttcgc cattgacggg gtgatcttat cgccgtagag
    17161 aatcgcttta ccgttaacgt tacgcgccgc acgaccaata gtctggatca acgaacgttc
    17221 agaacgcagg aagccttctt tgtcggcgtc gaggatcgcc accagcgaaa cctccggcat
    17281 atccagacct tcgcgcagta agttgatccc caccagcacg tcgaactcgc ccagacgcaa
    17341 gtcgcggata atctccatac gttcgacggt gtcgatatct gagtgaagat aacgcacgcg
    17401 ctcaccgtgt tcttcgagat attcggtaag atcttccgcc attcgcttgg tcagtgtggt
    17461 gaccagtacg cgttcgttaa ttgccgctcg ctgacgaatc tccgaaagaa gatcatcaac
    17521 ctgtgtcgcc actggccgca cttcgataat cggatcaagc aatccggttg gacgcaccac
    17581 ctgatccacc acatcgccgc cggatttttc cagctcataa ttacctggcg tcgccgaaac
    17641 atagatggtt tgcggcgcta atgcttcgaa ctcttcaaat ttcagcggac ggttatccag
    17701 cgctgatggc aggcggaagc cgtactccac cagtgtctct ttacgcgccc ggtcaccgcg
    17761 atacatgccg ccaatttgtg gaatggtgac gtgagattca tcgaccacca gcagcccatc
    17821 ggcaggcagg taatcaaaca gcgtcggcgg tggctcaccc ggtccacgac cggagaggaa
    17881 gcgcgagtag ttttcaatcc ccgaacagta gcccagctcg ttcatcatct ccagatcaaa
    17941 ctgggtacgc tgggtcagcc gctgctcttc cagcagtttg ttgttttcca atagcacttt
    18001 gcgtctggcg gccagctctt ctttgatctc ctccatcgcc tgtacgatgc gctcgcgcgg
    18061 tgtgacgtag tgcgttttcg ggtagatggt aaaacgtgga atggtggaaa caatctgccc
    18121 ggttagcggg tcaaataacg acaatcgttc cacttcctcg tcaaacagtt ccacgcgaag
    18181 tgcaatgtca tccgattctg ccgggaagat atcaatcacc tcgccacgaa cacggaaagt
    18241 accacgctgg aatgcttgat cattacgagc gtattgcagc tccgccagtc ggcgcagaat
    18301 cgcgcgctga tcgataatca taccgaccgt gagatggagc atcatcttga gatataaatc
    18361 aggatcgccc agaccataaa tcgcggaaac agacgcgacc acaacaacat cacgccgctc
    18421 cagcatcgct ttggtggcgg acaaacgcat ctgttcgata tgttcgttta ccgaggcatc
    18481 tttctcaatg aaggtgtcgg aactcggtac ataggcttct ggctgatagt agtcgtagta
    18541 ggagacgaaa tattccaccg cgttttccgg gaagaactct ttcatttcgc catacagctg
    18601 ggccgccagc gttttgttgg gcgcaagtac catggtaggg cgctgaaggt cagcaatgac
    18661 attggcaatg gtgaaggttt tcccggaacc agtcacacca agtaacgtct ggtgcgccag
    18721 gccatcttcc agcccctctt cgagacgtcg aatcgcctct ggctgatcgc cagaaggttt
    18781 aaaagcggaa ttcagtttga acggtttact catgagtcgc tacctgaagg agttgggcgg
    18841 gcaggtatgt aattttactc gtcgtactta attatgccaa caaattatac tggataaaaa
    18901 aacagttcat caccataata tttctgttac agcgtaaact ccgctgtcaa tcatgagtaa
    18961 aatttactct gtggcgagat aaaactcgga ccttaccggg ttatccccaa agcaacggct
    19021 tttttaacat ttgtcaagat gagtgatgtc agttttatgg caagagatgc ctgttcagtg
    19081 actgccattg caattatcta accagttaaa aaataaaaga tatttctttg agccgtcttt
    19141 aacgccaatt cgcgtgcaag ccgcgtattc tctcgcttgc ctcgtgtttt ctaactctaa
    19201 tacacatggt tatccacagg aatagtggat aactgtctgc agcccctatc ccccgccgcc
    19261 tgggcaattc ccacattcgc cgcatgatgc ggaagtaaaa tttttttcgt gattatttta
    19321 gaatttaatt ggttaaattg cagtcaatcg aagacgcgat ctcgctcgca atttaaccaa
    19381 atacaggatg gctacaacaa ggcaaggttt atgtactttc cggttgccgc attttctgga
    19441 ttttctgcaa gccaggggat ctctcccagc agcggcgcgg gaatcatgcg ggtgagcgtg
    19501 gtcatatatt cagcgtgacg ttttcccgga ggcgtgacat cgttcgccac ccaacccgcc
    19561 agagtcagcc cggcgtgttg tatcgcctgt gccgtcaaca tcgcgtgatt aatacagcca
    19621 agtttcacgc caaccaccag tattaccggc agttgttcct gtgttaccca atccgcaaaa
    19681 gtgaaagtgt cagaaagcgg cgtaaaccag ccgccagcgc cttccactaa cacccagtca
    19741 gcctgttgtt caagcgcgcg taatccggag ctcattaccg atgattctat cgacctgccc
    19801 tcttgcgcgc tgatgatgtg cggtgaggtg ggttctgcga aggtgtaagg atttaccgtt
    19861 gcgtaatcca gctgcaggct gctgttgcgc tgtaacgcca gcgcgtcact attacgtaaa
    19921 ccctccgggg tcttttcgct gccagaggcg accggtttat aacctgccgt ccgatagcct
    19981 gctgcctttg cggcttgtaa aagtgcacaa ctggcgacag ttttccccac ttcggtatcc
    20041 gttccggtga caaaataacg tttactcacg aacaatcact cccaaaaaaa gatgatacgt
    20101 cagaggatat cgcccctgtt gttgcggcca ggccagttgc aatcgctgca actgtgaacg
    20161 cgttaatatt cgctggtcgc gcccttcatg aagatgcgtg gcaccgatgc ctttcagcga
    20221 acgcatggca ctgagcgcat catcaaacca tagtgtgatg ggctgaatac gatgttgata
    20281 atgcacgccg ttcagcgact gttcgatttc atctggcggt aaaaagcgat tagcatgcgg
    20341 gcgctcgtcc accgcctgcc acgcctgatg cagttcgggt aacgatccct gcaccagcgt
    20401 ggtaaacgcg accacgcctc cggggcgcac cacccgatac agctcgcgga gtgccgttga
    20461 taaattaccg caccactgca ccgcgagatt gctccatgca agatcgaacg tcgcagtcgc
    20521 taacggcagg gattcgatat ctcccgccag ataatggtct gcagcatctt tctggcgggc
    20581 ttgcgcaagc attggcggcg agagatctaa cgccgtcacc tgcgcgtgac gctctcgcca
    20641 gtggcggctc atccagccag gcccacaccc cgcgtccagt acgtgggggt atttacgctg
    20701 tgggagcatt gccagtaaga catcagcact ctggcgctgt aactctgcgt gttgctcata
    20761 gtgcgcggct gcccgaccaa atgccgctgc aatggcttgt ttattaaccg ttgccatgca
    20821 gcacctccag cagacggtcg atatcctgca tttcatgcgc ggcggttagc gttaagcgca
    20881 gtcgcgcagt accagagggt acggttggcg ggcgaatcgc cgtgacccag cagccttgct
    20941 ggcgcagttt ttctgccagt tgtaacgcac ggctgttatc accgacgatc aatggctgga
    21001 tggcgctgca tgaatcagca agcgtaaacg gcaaatcctg tactccggca cgaaaacgcg
    21061 taatgagtgc cgccagtttt tcgcgccgtg catcaccctc atcactgcga atgaccgcca
    21121 gtgacgcacg taatgcctgc gcctgagcgg gcggcatact ggtgctgtag ataagatggc
    21181 gggcgaattg cagcagataa tccgccaccg tactggagca aagcaccgca gccccgctga
    21241 cgccaaatcc tttgccaaaa gtcactacca gcaattctgg ttttaccttt tgcaaccagc
    21301 aggtgccgcg cccctgctcc ccgataacgc ccgtgccgtg ggcatcatcg accatcaacc
    21361 agccattgtg ctgttgcgtt acctgctgga tttccgccag tggcgcacta tcgccgtcca
    21421 tgctgaacac gccttctgtc accaccagtt gctgccccgg acagggggaa gcaagcagtc
    21481 gcgccagatg agtgacatcg ttatgaacaa aacggcgaag ctgcgacggg cttaaactgg
    21541 cagcctccag caatgaggca tggctaagcc ggtcagcgac aatgcggtcc tcttttgcca
    21601 tcatcgcggc aatgaccgcc tgattagcgg caaaaccaga gataaatagc agtgcccgcg
    21661 aatagcccag ccactcggcc agttcttctt ccagcgcctg atgcgcaacg ctataaccgc
    21721 tgacgtgacc ggaaccgccg ctaccgacgc caaattgctc cgcaccctgc ttccaggcac
    21781 ggataatttg cggatgatgg cttaaaccta aataatcgtt actggaaaag ttcagatact
    21841 ggcgatcgtc cgccaccagc cagcgtccgg ctccttgcgc cactggataa cggcgacgca
    21901 gggcatcggc agcacgccgc gcatcgagcg ccgcgttgat tttctcctgc cagctcatag
    21961 tgctgccgcg ttgtaatatt cgtcagtgtc cggggtcatc agtgcctgct ccagacgctg
    22021 ctgttgttcg ttatcccctg ccagcacggc agtttgctgc ggatttagcc ccagtttgcg
    22081 aaacagttgc aggtctttat cttcttccgg attgggcgtg gttagcagtt tgcaaccgta
    22141 gaaaatcgag tttgcgcctg ccataaagca catcgcctga gtctgttcgt tcatctgctc
    22201 gcgtccggca gaaaggcgca cgtaagaggt cggcatcatg atccgcgcta cggcaatggt
    22261 gcgaataaaa tcaaaggcat cgacatcatc gttatctgcc agtggagtac ctttcacctt
    22321 caccagcatg ttgattggca cgctttccgg cggcgtcggc aggtttgcca gttgcagtaa
    22381 taatccggcg cgatctttta ccgtttcgcc taaacccaca atgccacctg agcagacttt
    22441 gatcccggca tcgcgcactt tttccagcgt atcaaggcgt tcctgataag tacgtgtggt
    22501 gatgatattg ccgtaaaact ccggcgaggt gtcgaggtta tggttgtagt aatccagccc
    22561 ggcattcgca aggcgctgcg cctgagattc actcaacgta cccagcgtca tacacgcctc
    22621 cagccccatc gcttttactc cctgcaccat ttgttccagg tatgacatat cgcgttcgtg
    22681 gggattcttc cacgccgcgc ccatacagaa gcgcgtcgat cctgccgctt tcgctttgcg
    22741 cgccgactcc agcacctgtt caacttccat caaccgctcg gcttccagcc cggttttgta
    22801 gcgcgagctt tgcgggcagt atttgcaatc ttccggacaa gctccggtct taatcgacag
    22861 caaggtgctg acctgcacct gacgaggatc gaaatgctgg cgatgcacct gctgcgcttc
    22921 aaacagcaga tccagcaacg gtttttcaaa taattctgtg acttgcgaca atgtccagcg
    22981 tgggcggtga gccatggggc ttctccaaaa cgtgtttttt gttgttaatt cggtgtagac
    23041 ttgtaaacct aaatcttttc aatttggttt acaagtcgat tatgacaacg gacgatcttg
    23101 cctttgacca acgccatatc tggcacccat acacatccat gacctccccg ctaccggttt
    23161 atccggtggc gagcgccgaa ggttgcgagc taattttgtc tgacggcaga cgcctggttg
    23221 acggtatgtc gtcctggtgg gcggcgatcc acggttacaa tcacccgcag cttaatgcgg
    23281 cgatgaagtc gcaaattgat gccatgtcgc atgtgatgtt tggcggtatc acccatgcgc
    23341 cagccattga gctgtgccgc aaactggtgg caatgacgcc gcaaccgctg gaatgcgttt
    23401 ttctcgcgga ctccggttcc gtagcggtgg aagtggcgat gaaaatggcg ttgcagtact
    23461 ggcaagccaa aggcgaagcg cgccagcatt ttctgacctt ccgcaatggt tatcatggcg
    23521 atacctttgg cgcgatgtcg gtgtgcgatc cggacaactc aatgcacagc ctgtggaaag
    23581 gctatctgcc agaaaacttg tttgctcccg ccccgcaaag ccgcatggat ggtgaatggg
    23641 atgagcgcga tatggtgggc tttgcccgcc tgatggcggc gcatcgtcat gaaatcgcag
    23701 cggtgatcat tgaaccgatt gtccagggcg caggcgggat gcgcatgtac catccggaat
    23761 ggttaaaacg aatccgcaaa atgtgcgatc gcgaaggtat cttgctgatt gccgacgaga
    23821 tcgccaccgg atttggtcgt accggcaaac tgtttgcctg tgaacatgca gaaatcgcgc
    23881 cggacatttt gtgcctcggt aaagccttaa ccggcggcac aatgaccctt tccgccacac
    23941 tcaccacgcg cgaggttgca gaaaccatca gtaacggcga agccggctgc tttatgcacg
    24001 ggccaacttt tatgggcaat ccgctggcct gtgcggctgc aaacgccagc ctggcgattc
    24061 tcgaatctgg cgactggcag caacaggtgg cggctattga agtacagctg cgcgagcaac
    24121 ttgcccccgc ccgtgatgcc gaaatggttg ccgatgtgcg cgtgctgggg gccattggcg
    24181 tagtcgaaac aactcgtccg gtgaatatgg cggcattgca aaaattcttt gtcgaacagg
    24241 gtgtctggat ccggcctttt ggcaaactga tttacctgat gccgccctat attattctcc
    24301 cgcaacagtt gcagcgtctg accgcagcgg ttaaccgcgc ggtacaggat gaaacatttt
    24361 tttgccaata acgggaagtc agcgtgagag tttctggcta cactttctgc aaactagaaa
    24421 ggagggttta tgaaactcat cagtaacgat ctgcgcgatg gcgataagtt gccgcatcgt
    24481 catgtcttta acggcatggg ttacgatggc gataatattt caccgcatct ggcgtgggat
    24541 gatgttcctg cgggaacgaa aagttttgtt gtcacctgct acgacccgga tgcgccaacc
    24601 ggctccggct ggtggcactg ggtagttgtt aatttacccg ctgatacccg cgtattaccg
    24661 caagggtttg gctctggtct ggtagcaatg ccagacggcg ttttgcagac gcgtaccgat
    24721 tttggtaaaa ccggatacga tggcgcagcg ccgccgaaag gcgaaaccca tcgctacatt
    24781 tttaccgttc acgcgctgga tgtagagcgt attgatgtcg atgaaggagc cagcggcgcg
    24841 atggtcgggt ttaacgttca tttccactct ctggcaagcg catcgattac cgcgatgttt
    24901 agttaatcac tctgccagat ggcgcaatgc tatctggtat cacttaaagg tattaaaaac
    24961 aactttttgt ctttttacct tcccgtttcg ctcaagttag tataaaaaag ctgaacgcga
    25021 aacagcaaaa cccaataata tcaatatatt acaatgcatt tagtctaaaa aatagactgc
    25081 ataatgctac aaaacacaac atatccagtc actatgaatc aactacttag atggtattag
    25141 tgacctgtaa cagagcatta gcgcaaggtg atttttgtct tcttgcgcta attttttgtc
    25201 aaacacatta cgtcgtttgc tgaatttaca accatgctca gtatctctat aagcgcagag
    25261 aaatgatgca gtggtgggtg ggctggcttg atgaaaaggt ggggtactcc acctgaacca
    25321 ctatcggaat agcacaaagc cttgcaatcc agtgcaaagt tttgtgtgcc tccgtttgcc
    25381 ccaccaatat atattcagca caagaaacac agctaaagca atggatgcag tcttacctgt
    25441 tggaatccca gactcactgt attgtcatca tgaataagag ctgagtcgtg cgggtaggag
    25501 gttctacggg gcatcccaat ctcttcgtgg aatttatttt ctgaaagcac ctcctctgaa
    25561 aaagccccca acccaacagt cctggcttct tcgagtaaaa gtggagagta tttttgtgat
    25621 tctgctcggg aactctcaac tttcaaacgc tccccattta aattgtggaa aacatggagc
    25681 aactcatgaa aaacaatagt cgcatgaaag tccaccacgc taatcccctc cccacaggga
    25741 tattcaactg cattcagatt acagtgaaaa tcggaaccag tcccccgatg gttctcagca
    25801 tctatatctc tatgtgccat aactcctagt ctggaagagt tgaggtgtat taccactgtt
    25861 tctgatttaa gtcgggatat tgattcaata gcattcagga gtacacgccc tgtctctgta
    25921 gagccaatct tatcgagtgc tgcttcaatc tgctgatata tattatgatc agtcccctgg
    25981 aaacgaagtt gcggattatt ttttatccat tgaacgctcg atgcttttac aagagactcg
    26041 atatctgtat ctgacattga gctcgacctt gatgactgat gtaataccaa gttgagggac
    26101 gtagggcgca tcaggactcc tgagaatgct acttattagc agcctgttta ccactctgtg
    26161 aaaatgattt ttttaaaagt gaccgtcaag gcggtcacca aacccgataa taaagcggat
    26221 catagccatt tgccagtcct cagcgacatt gcccatttcc gtgatccgcc aagaattgcc
    26281 aaccacacaa tttgttacca actccatcca tgcaacaaat ccactacact ggataaaatt
    26341 actaaatttt acttaatacc acactaataa gatcttgctt tccgccatga taagattgca
    26401 ttatttgact ttcactccat gaacggtcgg aaacattata agatgatata tctattggat
    26461 taaactcatt cttcgcgcgg tcatataaga catttgtctc tgttgtatcg acaaaaagaa
    26521 ttcctttttg ctccagtcta tcaaacatat cgtaaatagc atgctcagcc tgtgctggca
    26581 aggacgaaat atttaaaagc gattctccat ttattttatc cattctaata ccaataatat
    26641 caccattatt gccatatatt ttttctgcac tcccggcacc ataatattgg ttgaagcaac
    26701 gaacttcgct tgtcacctct tcattgcttt gagatgtagt aaacatcttc aggacttttg
    26761 ttgcatcctc agcatcttca tatactacag cgttcccccc tttgccaata acattaccag
    26821 gtactgacaa cctgttatag tccacactcg gtaactctgg cggtgcataa tcaacaggag
    26881 gtaaatcagg tctgttcgaa tgaacaatgc ctctttccat agcagcgctc actggtgatg
    26941 cattcagcat gacctcaatt ttcctgctaa tctccccctt aggccatccc agcctgtgca
    27001 acagattagt aaaacaacca ctatgacttt ctcttgtaac gcaaaactta ttatcggtgg
    27061 caacaacacg atatgttctg ttgccaacct ttacttgcgc cccattatca gaatgaacgg
    27121 cagcatccct tacagaggat aaaacacgat tatcaggcga agtcaggttt ctggttaaag
    27181 aattccatga acatcccaaa tttacagaat atggcgataa catacatttc aaccttcaaa
    27241 ataaacccat ctaaatttcc ttaacaaaca tccccctaac atggcaacaa aaattagaaa
    27301 tgaactcttg ctttttgtat ctggcaacta attcatcctt ccagcatccc atcaaacgaa
    27361 cgtttacgaa ctataacgca ggtattaccg gtcactcgat atctggagat acagacgtat
    27421 caacatgatt gagcggcata cgatactgct tccatgccgc cagtaacgat gtctgctcat
    27481 ccgtcgcaat atctaaatct gcagcatcct gaagtggtgc gatatactca ctgattacaa
    27541 gtaccagacc gatagattaa aaaaaacaca ataacacgct gcatttaaaa caaaaaattt
    27601 taaatatgat ttaatacaag ctttaagcct ttaattatca gttttattta ttttccagca
    27661 attgattaat caattgttta tatacaaatt attccttttc tttctctcac gcaaaattca
    27721 caaaatattg ttgctgcaat ggacctgtac aatagtgaat cacggtactt ttctgtgcac
    27781 aacgtactgc agtttcttta cgatttatag ctgtactgtt gaattatgta caatttgaat
    27841 ccatgcatga cgtgtgatgc ctattgtgca tttttccgag cctcttttta ctgggccgaa
    27901 gccgatgatg ctgatagtct cgttccggcc atgttcactt actacagtaa aattagggga
    27961 attgccttta catacaatgt tgcacgatta cccatcatca actccgttaa aatgtatgaa
    28021 tagtaacctt atgtcactgc aaagacgaat cattgctgat tgtgtttgtc cacatcccca
    28081 aatgtaaaaa tataaggggc gccatgatac agttggatct gatttgcatc ctgtaaatta
    28141 tcgttgatag ccaatccagg aaaatcatgt tgctggataa atccgtatcc tatgttagac
    28201 acagcggaat actctgtgaa atcaggactc gcctcatatc ggtcactgat cgtacccagc
    28261 ctttcgaaga aagccctctc attctcttca tgcctcatgg cagcctgtcg aagggcattc
    28321 aggttacgta gcctaagatg agcttcacgt tctggctctg catatccttt gaagtcggga
    28381 acactccatc ccagttcttg agcgacacga cgtgcgagaa tctcggtggg tcctaactct
    28441 atattactat ctccagatgg atcgctagat ccagtaacat gatgaataat ctcgtgaatc
    28501 agtccttcct gccacgatgg catctcataa gagtcagtat ctggcgcaac actaaaactg
    28561 acataaggct cttcgttatc gttttctcct gcttcgcaaa tggggaaaat tggctcttgc
    28621 ccgacatcat attcatataa ttcgttacag gttagtgatt gaatatcatc aattttgaca
    28681 gaagattctt cgttaagctc gtattcgttt ctgtatttaa tgcaaccaat gtgtaccttc
    28741 tcattatgaa tgccatagct tacggcatca cgaaatgtct gtgatcggct caaagcatca
    28801 agtacagtgt tgccgatcat atcgactgta tgctgatcga taagcctgct acggctgtca
    28861 tggacggcgc gtattacact ttcgtatacg ttgcttaaat ctgctgcgga aagtggtatt
    28921 cgcttaccta tatccaaaac gtaatccgca taggcatttt cagcacgatt aggagcaatg
    28981 ggagcagagt atcctgccgg ggcggaaaag ttgaagttgg actgtaatga gggaattttc
    29041 atgtttatat ctaataccct gcaaatcatt tattacaaca aaataaacta tatcaaattc
    29101 atttaccatg aactgcatgt atactgactt gtgttcatga ttatattagg gtggttaaac
    29161 tcaataaagt cacaaaaatg attataatta tgtaatggtg taaaattaaa atatttctta
    29221 actcctttcc ccaaaccatc ataatatgga tgagcatcta ctttactatg cataaaagaa
    29281 agtccctcaa gtagagcagg atgattacta cggttaacaa ttattgcact attttcaata
    29341 tttacactat cattacgacg atccacatgc attgaaattc catcaggagc atatattgta
    29401 ccaagcttac ctgtaagtat catatccata tcaagataga tacacccctc cccgaatgaa
    29461 ataccgtgat tctttgtatt atatgtgcac ctgaatatct ctcctgcttt taataaggct
    29521 aaatttctga agaaatcaaa ccatgcatga tttcttttct ttgcatacat agaaatcaaa
    29581 gaatcctgcg cctttgaaat ttccctcagc tctttctcta acagattcaa caagtattca
    29641 tctcttttgt cttcagttcg taacctttgt tcacatataa tatcatgata aatatctgat
    29701 agttttctgt catacatact gaagtcaaca tcttcccgat agattatcat tacattttca
    29761 aaatctcgtt ccaattttga aaaagcagtc ttttggttga ctgaaaaatc gccatcaaca
    29821 aaaataccta tcatacgatc actctctatc cttgccgcat ttgtgacatt atctaaatag
    29881 ggatgctgct tagtattaac tattggaacc tcatctttct tatatcgttc aggattaggt
    29941 tcaaaccact gaaaaagaat aggcgttttt tcatcaatga cctttaactc atattccttt
    30001 cctgcaaaag aaaccgtttg acagggtgaa ctctgcacta tatttactga gttatggaaa
    30061 gttgtcctta tcggtgaaag cattcgtcgt cctgtttatc catattttct tcacaactaa
    30121 ctctttaatc tattaattat attggcatac tcaaccacaa aacctccagc agttttgcca
    30181 tctttgcttt cctaacaaac atccaccgga catgacaaca aaaaccggag ccggactccg
    30241 gttttgtgaa gctgtcgggt tacttcatcc cgccaatatt ttcccacgtc ccgtcagcac
    30301 gcaggatttg cagcggtctt accacacact gtatctgctt tttatctgca tccagtatca
    30361 ccacctgtgt gattaccctg tcctgctccg gaataatgcc attctcatct gactccagaa
    30421 tgtctgccgg tcccagtcgc aattgtgctg taagtgaccg tccgttttca cagtcatcat
    30481 gctttccgca accgcacaga ctctgcataa gctttctcaa aatattcatg tcattctcca
    30541 gttctgcctg tatcactgcc cacttcatcc agtcccttaa catcctgcca tggcccgtca
    30601 ccaaatctga cctgcaaatg ctgaaacatc ccctgaacct gtgtggcatc tttggggtca
    30661 agaaaggtca gtccggtgat gagcgcacca tctgtacccg ggaaccagcc atagctgttt
    30721 gtctcaataa ttctcatcgg ccccagacgg aaccgtattt gtgtctcccc cgggtcgccc
    30781 ttcgccccct gaggtccggt agcccccacc gggccagccg cacctgtttc ccctttcggt
    30841 ccctgcggtc ctgccggacc tgttgccccg gtatctccct ttggcccctg tggacctgta
    30901 cttcctgtca gaccggtctc tccccgctct cccctgtcgc ctttcggccc ctgcggacct
    30961 gccggacctg tatctcctcc cggtccccgt tcaccggttg cccctgccgg accggtgtct
    31021 ccacgctctc ctttatctcc cttcggcccc tgaggacccg cgggacccgg ttcccccttt
    31081 ggtccgggag gccccaccac ggtgggaatt ctgtttacgg cgtcttccgc cgctattctg
    31141 ctttgttccg ctgactgtgc gctttctgct gattcccggg ctttttctgt tgcggtcgtt
    31201 gcatccctgg ctgcattacc ggctgcactt tctgccgtct tttttgacaa ctcggcatct
    31261 gttgcacttt gtaatgactc actggctttt tgagcggcct cagaggctga ggacgaggac
    31321 gcttcctctg acttctttgc agaggctgca ctttctgccg cctgccgggc tgactccgat
    31381 gcatcctctg ctgaagtatc agcatttgcg gcgctcgctt ccgccttact ggctgatatg
    31441 ccggcattcc tcgcggacgt ctctgcttct ccggcattct tcttcgcctc ctctgcgtga
    31501 cgcgctgctt cttccaccat ctgttcaaaa cgacgcagtg cctccggacg ggcatcatcc
    31561 tccgtcatgg caccgagaaa atcattcagc gtacccggtt gagaatcttc atacacggag
    31621 atggtcccgg catgtgatgg cgggaatccc tccaccaaca gagtgacgct gtactgacca
    31681 tactcgacgt ccatgctgta acgccctgcc tcatccggat tttcagaggc caccgtgttc
    31741 accaccaccg tggtgctgtt acgtctggct ttcagttgaa tggtgcagtt ctgtattggt
    31801 tttcccgcac catctttcag cacgcctgat atttgtactg ctgccatacc cactccacaa
    31861 aaaagcccgc ctgaaccggc gggctgtcat aacgctgtgt tactaatcaa aatttataac
    31921 cgacacccac gatgaaaccg tcagtgcgcc agtcaccgct gccggagcct tcataagcga
    31981 catcaacggc cactgattcg gtcgggttaa actgcacgcc agccccccac gccagagatg
    32041 tgttgctgta gcgagcgtca tcacttccgg tcagcacgtc gtgcgttttc ctcttgttgt
    32101 cagttacgcg gaaataatcc ccggaaaaag tcgacacacg gctgtaagcc acacccgcca
    32161 tcgcatacgc actgaaccat tcattcacac gcacagacgg ccctgccatc acgctgaacc
    32221 agcggttacg cacgtaatcc tcatgccagc gggtatcgct gtaatgcgtt ttttgctcat
    32281 cttcggcgtt ggcataactg aaggacgtaa tcagccccag tgtgtccgta aactcataac
    32341 ggtatttcac gttaatcccg ttcagatcat cgctgccggg agcgtccgta ctggcatgaa
    32401 gatatcctgc gctcagcgtg gactgatgct cagacgccca tgcaggcaca ccggatacgg
    32461 ccagacaaat ggctgcggac aaaattgctg cacaaacttt acgcataatt acctcccgat
    32521 tttctgcaat aaaaaaggcg ccatttctgg cgcccgttat tagggttata aatatttcaa
    32581 cggatactga tgccggaagc cgcttttttg gtcacaatca ccgtacagtc ggtgatatta
    32641 cctgcccgct gattgccttt atggaaaacc ttaaactcca gagtgacgct tcccctgcca
    32701 ctcggcatat caataaccgc actgtagcta ccgggaatgg cccctttagt ttctctggat
    32761 gcgattaata caccgttttt gcgaacttca aaaccataac ccgtgtatct tgtacctccc
    32821 gggttattac cacttcccgg atcgctatac gctattccgt taaagataat gggcggaata
    32881 atgatttgac ggtcaaagtt atgatcatcg ctgatggtga ctgtaaccgt cccgtttggt
    32941 gtttccgtgt taccccacgt accagcctgt ttcggaaatg atttggatac agctttaacg
    33001 aagtcacctc tgacctgagt cgcctccagc atgcccttaa tcgtacagtt ttcatttacc
    33061 gtgacgttgt tgagcgtccc ggagttcgca ttcacactgc cactgatatc cgcattttta
    33121 gcggtcagct ttccgtctga tgtcagggaa aat
//
LOCUS       DAZFAB010000042        33108 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZFAB010000042 DAZFAB010000000
VERSION     DAZFAB010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33108
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.042"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            172..318
                     /locus_tag="ACHJWA_002331"
                     /pseudo
     CDS             172..318
                     /locus_tag="ACHJWA_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
     gene            945..1244
                     /locus_tag="ACHJWA_002332"
     CDS             945..1244
                     /locus_tag="ACHJWA_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4308919.1"
                     /translation="MGNGNFWQLFLGKEPYSKKSDFVKAWLLWWAITILVLFILSLTT
                     TLNLRSIGIGLSFGFCQILGRYTMSLGWSKYWGVLGGFPVTNIVLFLILIFFKKR"
     gene            complement(1291..2178)
                     /gene="rarD"
                     /locus_tag="ACHJWA_002333"
     CDS             complement(1291..2178)
                     /gene="rarD"
                     /locus_tag="ACHJWA_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFO4308920.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(2230..2697)
                     /gene="yigI"
                     /locus_tag="ACHJWA_002334"
     CDS             complement(2230..2697)
                     /gene="yigI"
                     /locus_tag="ACHJWA_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFO4308921.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            2862..3731
                     /gene="pldA"
                     /locus_tag="ACHJWA_002335"
     CDS             2862..3731
                     /gene="pldA"
                     /locus_tag="ACHJWA_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFO4308922.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            3864..5693
                     /gene="recQ"
                     /locus_tag="ACHJWA_002336"
     CDS             3864..5693
                     /gene="recQ"
                     /locus_tag="ACHJWA_002336"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFO4308923.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            5757..6377
                     /gene="rhtC"
                     /locus_tag="ACHJWA_002337"
     CDS             5757..6377
                     /gene="rhtC"
                     /locus_tag="ACHJWA_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFO4308924.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(6439..7059)
                     /gene="rhtB"
                     /locus_tag="ACHJWA_002338"
     CDS             complement(6439..7059)
                     /gene="rhtB"
                     /locus_tag="ACHJWA_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFO4308925.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLDVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            7170..8192
                     /gene="pldB"
                     /locus_tag="ACHJWA_002339"
     CDS             7170..8192
                     /gene="pldB"
                     /locus_tag="ACHJWA_002339"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFO4308926.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     IHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            8200..9000
                     /gene="yigL"
                     /locus_tag="ACHJWA_002340"
     CDS             8200..9000
                     /gene="yigL"
                     /locus_tag="ACHJWA_002340"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFO4308927.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            9076..9975
                     /gene="bioP"
                     /locus_tag="ACHJWA_002341"
     CDS             9076..9975
                     /gene="bioP"
                     /locus_tag="ACHJWA_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFO4308928.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(9863..10816)
                     /gene="metR"
                     /locus_tag="ACHJWA_002342"
     CDS             complement(9863..10816)
                     /gene="metR"
                     /locus_tag="ACHJWA_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFO4308929.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            11052..13313
                     /gene="metE"
                     /locus_tag="ACHJWA_002343"
     CDS             11052..13313
                     /gene="metE"
                     /locus_tag="ACHJWA_002343"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFO4308930.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPARHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLDEVDEALALGHKVKPV
                     LLGPITWLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKCGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYVQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSMTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(13353..14168)
                     /gene="ysgA"
                     /locus_tag="ACHJWA_002344"
     CDS             complement(13353..14168)
                     /gene="ysgA"
                     /locus_tag="ACHJWA_002344"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFO4308931.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGLDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAASAEDGWQRMLEWFKQYGGKKSL"
     gene            14430..15191
                     /gene="udp"
                     /locus_tag="ACHJWA_002345"
     CDS             14430..15191
                     /gene="udp"
                     /locus_tag="ACHJWA_002345"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFO4308932.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            15332..16759
                     /gene="rmuC"
                     /locus_tag="ACHJWA_002346"
     CDS             15332..16759
                     /gene="rmuC"
                     /locus_tag="ACHJWA_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFO4308933.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            16854..17609
                     /gene="ubiE"
                     /locus_tag="ACHJWA_002347"
     CDS             16854..17609
                     /gene="ubiE"
                     /locus_tag="ACHJWA_002347"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFO4308934.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            17623..18228
                     /gene="ubiJ"
                     /locus_tag="ACHJWA_002348"
     CDS             17623..18228
                     /gene="ubiJ"
                     /locus_tag="ACHJWA_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFO4308935.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLAALIRSGELEVQGDI
                     QVVQNFVALAGLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALIKRLEKLEAK"
     gene            18225..19865
                     /gene="ubiB"
                     /locus_tag="ACHJWA_002349"
     CDS             18225..19865
                     /gene="ubiB"
                     /locus_tag="ACHJWA_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFO4308936.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            19944..20213
                     /gene="tatA"
                     /locus_tag="ACHJWA_002350"
     CDS             19944..20213
                     /gene="tatA"
                     /locus_tag="ACHJWA_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFO4308937.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            20217..20732
                     /gene="tatB"
                     /locus_tag="ACHJWA_002351"
     CDS             20217..20732
                     /gene="tatB"
                     /locus_tag="ACHJWA_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFO4308938.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            20735..21511
                     /gene="tatC"
                     /locus_tag="ACHJWA_002352"
     CDS             20735..21511
                     /gene="tatC"
                     /locus_tag="ACHJWA_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFO4308939.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            21553..22335
                     /gene="tatD"
                     /locus_tag="ACHJWA_002353"
     CDS             21553..22335
                     /gene="tatD"
                     /locus_tag="ACHJWA_002353"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFO4308940.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(22332..22820)
                     /gene="rfaH"
                     /locus_tag="ACHJWA_002354"
     CDS             complement(22332..22820)
                     /gene="rfaH"
                     /locus_tag="ACHJWA_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFO4308941.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            22987..24480
                     /gene="ubiD"
                     /locus_tag="ACHJWA_002355"
     CDS             22987..24480
                     /gene="ubiD"
                     /locus_tag="ACHJWA_002355"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFO4308942.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPIS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGEMAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            24526..25227
                     /gene="fre"
                     /locus_tag="ACHJWA_002356"
     CDS             24526..25227
                     /gene="fre"
                     /locus_tag="ACHJWA_002356"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFO4308943.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(25419..26582)
                     /gene="fadA"
                     /locus_tag="ACHJWA_002357"
     CDS             complement(25419..26582)
                     /gene="fadA"
                     /locus_tag="ACHJWA_002357"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFO4308944.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSVSDIGVFEMNEAFAAQILPCIKDLGLIEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(26592..28781)
                     /gene="fadB"
                     /locus_tag="ACHJWA_002358"
     CDS             complement(26592..28781)
                     /gene="fadB"
                     /locus_tag="ACHJWA_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFO4308945.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVIMKDINDKSLALGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFER
                     VDVVVEAIVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWHYKEDSKGKPKKEEDAAVDDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            28971..30302
                     /gene="pepQ"
                     /locus_tag="ACHJWA_002359"
     CDS             28971..30302
                     /gene="pepQ"
                     /locus_tag="ACHJWA_002359"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFO4308946.1"
                     /translation="MESLASLYKNHIATLQERTRDALTRFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQASEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            30302..30916
                     /gene="yigZ"
                     /locus_tag="ACHJWA_002360"
     CDS             30302..30916
                     /gene="yigZ"
                     /locus_tag="ACHJWA_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFO4308947.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITLLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            30955..32406
                     /gene="trkH"
                     /locus_tag="ACHJWA_002361"
     CDS             30955..32406
                     /gene="trkH"
                     /locus_tag="ACHJWA_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFO4308948.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            32418..32963
                     /gene="hemG"
                     /locus_tag="ACHJWA_002362"
     CDS             32418..32963
                     /gene="hemG"
                     /locus_tag="ACHJWA_002362"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFO4308949.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7784 a         8281 c         8956 g         8087 t
ORIGIN      
        1 ttatatttaa gctcaatatc aatttgagaa caaatgctag ggtaaaaaaa tatttaaaaa
       61 agaagtacac ttcacatttt ttcattacat ctctgaagag aacgtcgtta atgattggtc
      121 ttgctcattg ttaataacaa aatattgaat ttaccgatta ggaaactttt tcctacgccg
      181 attgcctcca gctatggcat gaactttgag tttatgccag aactgaagtg gagtttcggc
      241 tacccaggcg cgattatctt tatgatcctc gcgggcctgg caccgtatct gtactttaag
      301 cgcaagaact ggctgtaact atacgatgtc gctgtgagta agttagtgtg ctgacagcga
      361 cattttagca gagggaatcg cagataacat aattgttgtg aataatatcc atgccacgtt
      421 gagttggacg cttaatgact cacatctcac cccttcaatt gctgcagaga taatagaaaa
      481 aagtgtcaaa cccaatgaaa tcaatacaaa gtagattgcc ttacatccag tatctcttat
      541 tctttttgct ccaatattga tatttacata ggtattatta ataaccagaa taaaactcaa
      601 agccaggttg atatcattca aagttattaa tattgaccat gatgttaatg tatagattat
      661 gaactgaaag cgtgataaac gctcaagctt ggatgggtag aatatatttc tgatggatga
      721 tttgaacatt ttttacattg ttttatatat ataattgttt tgaatatata ttacctgaat
      781 tatgtggttt gcatatatat atgcaatata gcagtctttg cattgataac tatacagtag
      841 taatttagag tgtgtaagtt tttattgatg gggtatattt taattaaata taattggaat
      901 gtatttttat tctataaaca atatcttggg tgaatggatt ctatatggga aatggtaact
      961 tttggcagtt gttcttgggt aaagaacctt acagcaaaaa atctgatttt gttaaggcat
     1021 ggttattgtg gtgggctatt accatccttg ttctatttat tttaagcctg actacgacat
     1081 taaatttaag aagtataggt atagggttaa gttttgggtt ttgtcaaatc cttggtagat
     1141 atacgatgtc tctaggatgg agtaagtact ggggagtttt ggggggattt cctgtaacaa
     1201 atattgttct ttttcttatc ttaatttttt tcaaaaaaag ataaataagt cagtgggtag
     1261 gctctttaaa cggcctaccc atgttaattc ttaacttttt ctacgctgcg tataaatcgc
     1321 atccatcaca aaaattgcca gcgccaccca aataaaggtg aaagtcacca tcttatcggc
     1381 acccggtttt tcaccataaa acgtcacagc cagcaggaac atcagcgtcg ggccaatgta
     1441 ctggaaaaag cctaacgttg agagacgaag gcgcgtggca gcggcggtaa aacacaacag
     1501 cggtacggta gtgacaatac cggcggcgat cagcagtaaa ttcagcgaca tcgggttttg
     1561 ccccatatgg ctggttgagc tgtcggcaat agcaaacagg taaatcgccg ccacgggcag
     1621 cagccacatg gtttcaatta acatgccggt ttgtgcttct accgcaatct tcttgcgcac
     1681 caggccgtag aaggcaaaac taaatgccag ccccagcgcg ataataggta gcgaaccaaa
     1741 agtccacagc tggactaaca cgccacatat cgccagaatc accgccagcc attgcatccg
     1801 gcggaatcgc tcgccgagga aaatcatccc cagcacaatg ttcaccagcg ggttaataaa
     1861 gtaaccaagg ctcgcttcca gcatatggtg attgttcacc gcccagataa acagtagcca
     1921 gttgccacca atcagcacgg cagagactgc cagcataaaa attttctgtg gcgtctgaat
     1981 cagcgttttt aaataggacc actggcggca aatgctcatc agcaccacca taaagaaaaa
     2041 cgaccagatc acgcgatgcg tcaggatctc atcggcgggc acgtagtaaa tcaacttgaa
     2101 gtacgctggg gctatacccc aaataaaata agcggcaaga gcgagtaata cgccctgccg
     2161 cgtttgtttt gcatccatcg ggaatactca tttttaattg gtaacagcag tttacctgct
     2221 tttatgtctt caacctacca tataggtggc ggtggcactg gcaatataaa gctgttcttc
     2281 attgtgtaat tcaacgcggg cgacggcgac tttattgcct gcacgcaaca ggctactggt
     2341 agcagtaaaa cgctcgcctc tgcctgggcg cagataatcg acgcgaagat caatggtccc
     2401 catccgcgat agccgctggc gtagttcatc ttcactgatg gtttcgtggc gggttaaggt
     2461 acttcctacg cacaccagac cggcggcgac atccagcgcc gacgcaatga ccccgccgtg
     2521 caaaatgctt tgcgcccagt tgcccaccat cattggctga tttttaaagg ccagctgtgc
     2581 gaactctttt tcgtaacgct ccagttccat ccccaatgcg cggttaaatg gcatgtgata
     2641 aacaaacatc tcacccacta atttcagggc ttgttcagcg gtcagtacgg cagacatatc
     2701 atccttacac ttcattggtt aatgaaatgt tgattttatg attctttgtt gttggtttct
     2761 actttaggaa gggataacta acggcaatgg agttaagtat gtaaaatacc ccgcagaaaa
     2821 acattcacct tatcaataat tcgttacgga gaacacgacc gatgcggact ctgcagggct
     2881 ggttgttgcc ggtgtttatg ttgcctatgg cagtatatgc acaagaggca acggtgaaag
     2941 aggtgcatga cgcgccagcg gtgcgtggca gtattatcgc caatatgctg caggagcatg
     3001 acaatccgtt cacgctctat ccttatgaca ccaactacct catctacacc caaaccagcg
     3061 atctgaacaa agaagcgatt gccagttacg actgggcgga aaatgcgcgt aaggatgaag
     3121 taaagtttca gttgagcctg gcatttccgc tgtggcgtgg gattttaggc ccgaactcgg
     3181 tgttgggtgc gtcttatacg caaaaatcct ggtggcaact gtccaatagc gaagagtctt
     3241 caccgtttcg tgaaaccaac tacgaaccgc aattgttcct cggttttgcc accgattacc
     3301 gttttgcagg ttggacgctg cgcgatgtgg agatggggta taaccacgac tctaacgggc
     3361 gttcagaccc gacctcccgc agctggaacc gcctttatac tcgcctgatg gcagaaaacg
     3421 gtaactggct ggtagaagtg aagccgtggt atgtggtggg taatactgac gataacccgg
     3481 atatcaccaa atatatgggt tactaccagc ttaaaatcgg ctatcacctc ggtgatgcgg
     3541 tgctcagtgc gaaaggacag tacaactgga acaccggcta cggcggcgcg gagttaggct
     3601 taagttaccc gatcaccaaa catgtgcgcc tttatactca ggtttacagc ggctatggcg
     3661 aatcgctcat cgactataac ttcaaccaga cccgtgtcgg tgtgggggtt atgctaaacg
     3721 atttgttttg atgaacggtt gagtggttgg caaatctgga atccagcatc caggattacc
     3781 ctctcagaga ctaaaagcat tgcagtttct cgcgcaggcg ctgaaaatag cgcctgtttt
     3841 tatttcaggc aatcggggtg aatgtggcgc aggcggaagt gttgaatctg gagtccggag
     3901 ctaaacaggt tttacaagaa acctttggct accaacagtt tcgccccggc caggaagaaa
     3961 ttatcgacac tgtgctttcc ggtcgcgatt gcctggtcgt catgcccacc ggtggcggaa
     4021 aatccctttg ctatcaaatt cctgccttat tgctaaacgg ccttaccgtg gttgtttcac
     4081 cgctgatttc gttgatgaaa gatcaggtgg atcaactgca agccaacggc gtggcggcgg
     4141 cgtgccttaa ctcgacgcaa acccgcgaac agcaacttga agtgatgaca ggctgccgca
     4201 ccgggcaaat tcgtctgctt tatatcgccc cggaacgcct gatgctggat aactttcttg
     4261 agcatctggc gcactggaat ccggtgttat tagccgttga tgaagcgcac tgtatctccc
     4321 aatggggcca cgatttccgc ccggaatatg ccgcgctcgg tcagttgcgc cagcggttcc
     4381 cgacgctgcc gtttatggcg ctgaccgcca cagccgacga caccacgcgc caggatatcg
     4441 tgcgcctgct ggggctgaac gatccgctga ttcaaatcag cagttttgac cgtccgaata
     4501 ttcgctacat gctgatggag aaattcaaac cgctcgatca gttgatgcgc tacgtgcagg
     4561 aacagcgcgg taagtccggc attatctact gcaacagccg ggcgaaagta gaagacaccg
     4621 ctgcgcgcct gcaaagcaag ggtattagcg cggcggccta tcatgccggg ctggaaaata
     4681 atgtccgcgc cgacgtgcag gagaaattcc agcgcgatga cctgcaaatt gtggtggcga
     4741 cggtggcgtt cggcatgggc atcaataaac caaacgttcg cttcgtggtc cactttgata
     4801 ttccgcgcaa tatcgaatcc tattatcagg aaaccggtcg cgccgggcgt gatggcctgc
     4861 ctgcggaagc gatgttgttt tacgatccag ctgatatggc gtggctgcgc cgttgtctgg
     4921 aagagaagcc gcaggggcag ttgcaggata tcgagcgcca caaactcaat gcgatgggcg
     4981 cgtttgccga agcgcaaact tgccgtcgtc tggtattgct gaactatttt ggcgaagggc
     5041 gtcaggagcc gtgcgggaac tgcgatatct gcctcgatcc gccgaaacag tacgacggtt
     5101 caaccgatgc tcagattgcc ctttccacca ttggtcgtgt gaatcagcgg tttgggatgg
     5161 gttatgtggt ggaagtgatt cgtggtgcta ataaccagcg tatccgcgac tatggtcatg
     5221 acaaactgaa agtctatggc atgggccgtg ataaaagcca tgaacattgg gtgagcgtga
     5281 tccgccagct gattcacctc ggcctggtga cgcaaaatat tgcccagcat tctgccctac
     5341 aactgacaga ggccgcgcgc ccggtgctgc gcggcgaatc ctctttacaa cttgccgtgc
     5401 cgcgtatcgt ggcgctcaaa ccgaaagcga tgcagaaatc gttcggcggc aactatgatc
     5461 gcaaactgtt cgccaaatta cgcaaactgc gtaaatcgat tgccgatgaa agtaatgtcc
     5521 cgccgtacgt ggtgtttaac gacgcaacct tgattgagat ggctgaacag atgccgatca
     5581 ccgccagcga aatgctcagc gttaacggcg ttgggatgcg caagctggaa cgctttggta
     5641 aaccgtttat ggcgctgatt cgtgcgcatg tcgatggcga cgacgaagag tagtcagcag
     5701 cataaaaaag tgccaggatg aagactccgt aaacgtttcc cccgcgagtc aaatgtatgt
     5761 tgatgttatt tctcaccgtc gccatggtgc acattgtggc gcttatgagt cccggtcccg
     5821 atttcttttt tgtctctcag accgctgtca gtcgttcccg taaagaagcg atgatgggcg
     5881 tgctgggtat tacctgcggc gttatggttt gggcagggat tgcgttgctt ggcctgcatt
     5941 tgattatcga aaaaatggcc tggctgcata cgctgattat ggttggcggt ggcctgtatc
     6001 tctgctggat gggttaccag atgctgcgcg gcgcgctgaa aaaagaggcg gtttctgcac
     6061 ctgcgccaca ggtcgagctg gcgaaaagtg ggcgcagttt cctgaaaggt ttactgacca
     6121 atctcgctaa tccgaaagcg attatctact ttggctcggt gttctcattg tttgtcggtg
     6181 ataacgttgg cactaccgcg cgctggggca ttttcgcgtt gattattgtc gaaacgctgg
     6241 cgtggtttac tgtggtggcc agcctgtttg ccctgccgca aatgcgccgt ggttatcaac
     6301 gtctggcgaa gtggattgat ggttttgccg gggcgttatt tgccggattt ggcattcatt
     6361 tgattatttc gcggtaatgc cagacgcgtc ttcagagtaa gtcggataag gcgtttacgc
     6421 cgcatccgac attatttttc acgcatgcct cgccgatgct aacagcgctc ccaccagcat
     6481 aaacaacgag ccgaaaatct tattcagcgc cttcatctgc tttggtcctt taatccatag
     6541 agcaatccgt tgagcaaggg tggcgtaacc gatcatcaca ataatatcga ccacaatagt
     6601 ggtgacatcg agcacgatat actgcatcag ttgcggctgt tgcggcatga tgaattgcgg
     6661 aaatagcgcc gccagaaaca caatactttt gggattggtg agattcacaa aaactgcgcg
     6721 ctggaacaaa tgtcgacgcg attgagtaga ggccagcgat ttaagatcaa ttgcgccagc
     6781 ggcgcgccat tgctgtattc ccagccaaat caagtaagcc gcgcctgccc acttcaacac
     6841 ttcaaacgca atcactgagc gggaaaatag cgtccccaac cccacgccaa ccagcacaat
     6901 atgaatcgcc agtccggtct gaagcccggc aatagacgcc accgcgccgc gataaccgtg
     6961 gttgagcgag gtggtcatag tgttgattgc accagagcct ggcgacaggc ttaaaatgat
     7021 tgatgtcagt aggtaggcaa accaccattc taaggtcatg atgaactccc ggtgtgtcta
     7081 tttttgtgcc acaatacgct actgtcgcag cgttgtgtca ggcacgctaa aaaaaacgat
     7141 tttacgtggt ttaagaggca gattacccga tgtttcagca gcaaaaagac tgggaaacaa
     7201 gagaaaacgc gtttgctgct tttaccatgg gaccgctgac tgatttctgg cgtcagcgtg
     7261 atgaagcaga gtttactggt gtggatgaca ttccggtgcg ctttgtccgt tttcgcgcac
     7321 agcaccatga ccgggtggta gtcatctgcc cggggcgtat tgagagttac gtaaaatatg
     7381 cggaactggc ctacgatttg ttccatttgg ggtttgatgt cttaatcatc gaccatcgcg
     7441 ggcagggacg ttccggtcgc ctgttagccg atccgcatct cgggcatgtt aatcgcttta
     7501 atgattatgt tgatgatctg gcggcattct ggcaacagga ggtccagccc ggtccgtggc
     7561 gtaaacgcta tatactggca cattcgatgg gcggtgcgat ctccacatta tttctgcaac
     7621 gccatccagg tgtatgtgac gccattgcgc taactgcgcc aatgtttggg atcgtgattc
     7681 gtatgccgtc atttatggca cggcagatcc tcaactgggc cgaagcgcat ccacgtttcc
     7741 gtgatggcta tgcaataggc accgggcgct ggcgcgcgtt gccgtttgct atcaacgtac
     7801 tgacccacag cagacagcga tatcgacgta acttacgctt ctatgctgat gacccaacga
     7861 ttcgcgtcgg tgggccgacc taccattggg tacgcgaaag tattctggct ggcgaacagg
     7921 tgttagccgg tgcgggtgat gacgccacgc caacgcttct cttgcaggct gaagaggaac
     7981 gcgtggtgga taaccgcatt catgaccgtt tttgtgaact ccgcaccgcc gcgggccatc
     8041 ctgtcgaagg gggacgaccg ctggtaatta aaggtgcgta ccatgagatc ctttttgaaa
     8101 aggacgcaat gcgctcagtt gcgctccacg ccatcgttga ttttttcaac aggcataact
     8161 cacccagcgg aaaccgctct acagaggttt aaatttctta tgtaccaggt tgttgcgtct
     8221 gatttagatg gcacgttact ttctcccgac catacattat ccccttacgc caaagaaacc
     8281 ctgaagctgc tcaccgcgcg cggcatcaac tttgtgtttg cgaccggtcg tcaccacgtt
     8341 gatgtggggc aaattcgcga taatctggag attaagtctt acatgattac ctccaatggt
     8401 gcgcgcgttc acgatctgga tggtaatctg atttttgctc ataacctgga tcgcgacatt
     8461 gccagcgatc tgtttggcgt agtcaacgac aatccggaca tcattactaa cgtttatcgc
     8521 gacgacgaat ggtttatgaa tcgccatcgc ccggaagaga tgcgcttttt taaagaagcg
     8581 gtgttcaaat atgcgctgta tgagcctgga ttactggagc cggaaggcgt cagcaaagtg
     8641 ttcttcacct gcgattccca tgaacaactg ctgccgctgg agcaggcgat taacgctcgt
     8701 tggggcgatc gcgtcaacgt cagtttctct accttaacct gtctggaagt gatggcgggc
     8761 ggcgtttcaa aaggccatgc gctggaagcg gtggcgaaga aactgggcta cagcctgaag
     8821 gattgtattg cgtttggtga cgggatgaac gatgccgaaa tgctgtcgat ggcggggaaa
     8881 ggctgcatta tgggcagtgc gcaccagcgt ctgaaagacc ttcatcccga gctggaagtg
     8941 attggtacta atgccgacga cgcggtgccg cattatctgc gtaaactcta tttatcgtaa
     9001 tcgttcttta tttggtcagt tgtcaacctg atacttcgct acaatggata ctcgttaatc
     9061 aaagagtttt ccattgtggc gctacttatc atcaccacga ttctgtgggc cttctccttt
     9121 agcttttatg gcgagtacct tgcggggcac gtcgatagct attttgcggt gctggtgcgc
     9181 gttggcctgg cggcactcgt ttttctgccg tttctgcgta cccgtggcaa tagcctgaaa
     9241 acagtcggcc tgtatatgct ggtgggcgcg atgcagctcg gcgtgatgta tatgctgagt
     9301 ttccgcgctt atctctacct gacggtttcc gaactgctgc tgttcaccgt actgacgccg
     9361 ctctacatca cgctgattta tgacatcatg agtaagcgcc gtctgcgctg gggatatgct
     9421 tttagtgcct tgttggcggt gattggtgcc gggattattc gctatgatca ggtcaccgac
     9481 catttctgga ctggcttgtt gctggtgcaa ctctccaata tcacttttgc catcggcatg
     9541 gtgggttaca aacgcctgat ggaaactcgc ccgatgccgc agcataacgc ctttgcgtgg
     9601 ttctatcttg gcgcgttcct ggtggcggta attgcatggt tcttgctggg aaatgcgcag
     9661 aaaatgccgc aaaccacgtt gcaatggggc attctggtgt ttcttggcgt ggtggcttcc
     9721 gggattggct actttatgtg gaactacggc gcaacccaag tagacgccgg aacgctgggc
     9781 attatgaata atatgcacgt tccggcaggg ctgctggtta acctggctat ctggcaccaa
     9841 cagccgcact ggccaacgtt tattacaggc gcgctggtga tcctggcctc actgtgggtg
     9901 catcgtaagt gggtcgctcc gcgctcttca caaacggcag atgatcgcag gcgtgattgc
     9961 gcgctgagcg aataaacgct tccgtaactg gctgacgctg ctcgccatcg cgcacggcgg
    10021 cgtacagtcg gctccacaag ccttcgccca gggttttggt caccaccaga ccctggcgct
    10081 caaaactctc tactacccaa tgtggtagcg cggcaatacc catccgcgcg gcaaccatct
    10141 gaatcaacaa taaggtgtta tcgacgcttt tcagcgacgg gctaacgcct gcgggctgga
    10201 gaaaatgccg ccagacatcc agtcggctac gctgcaccgg ataaatcaac agcgtttcgc
    10261 tggcgagatc ttccggcgtg attcgcgttt tcgccgccag tggatggtca ggtgctaaca
    10321 ccagacgcac ttcatagtcg aacatcggcg aataatgcag gccactacgc ggcagaatat
    10381 cggaggtcat caccagatcc agctcgccct gctgcaacgc aggctgcggg tcaaacgtta
    10441 cgcccgattt aaaatccatc tccacctgcg gccagttctt atggaaattt tctaatgcgg
    10501 gcgtcagcca ctgaatacag ctatggcact caatggcaat gcgcaggcgc gtctgttgcg
    10561 gttcattgca ggcttgcagc gcctggctaa tttgcggcag cacctggttt gccagttgca
    10621 acaggatttc cccctgtgga gtaaagcgta gcggctggct cttacgcaca aatagccgga
    10681 agccaaggcg ttgttccaga tcgctaaact ggtgagacag ggcggattgc gtttgatgca
    10741 acgtcgccgc agcggctgcg agagagccgc agttccgcaa cgcttgtagc gttttcaggt
    10801 gttttacttc gatcatgaaa gtccttcact tcggcatgaa taatttgcgc ttgaggaata
    10861 tacagtaacc gccaattatg gatgtgtaaa catctggacg gctaaaatcc ttcgtctttt
    10921 aaatttatgg tgcgttggct gcgtttcttc accccggtca cttacttcag taagctcccg
    10981 gggatgaata aacttgccgc cttccctaaa ttcaaaatcc ataggattta catataatta
    11041 gaggaagaaa aatgacaata ttgaatcaca ccctcggttt ccctcgcgtt ggcctgcgtc
    11101 gcgagctgaa aaaagcgcaa gagagttatt gggcggggaa ctccacgcgt gaagaactgc
    11161 tggcggtagg gcgtgaattg cgtgctcgtc actgggatca acaaaagcaa gcgggtatcg
    11221 acctgctgcc ggtgggcgat tttgcctggt acgatcatgt actgaccacc agtctgctgc
    11281 tgggtaacgt tccggcgcgt catcagaaca aagatggttc ggtagatatc gacaccctgt
    11341 tccgtattgg tcgtggacgt gcgccgactg gcgaacctgc ggcggcagcg gaaatgacca
    11401 aatggtttaa caccaactat cactacatgg tgccggagtt cgttaaaggc caacagttca
    11461 aactgacctg gacgcagctg ctggacgaag tggacgaggc gctggcgctg ggccacaagg
    11521 tgaaacctgt gctgctgggg ccgattacct ggctgtggct ggggaaagtg aaaggtgaac
    11581 agtttgaccg cctgagcctg ctgaacgaca ttctgccggt ttatcagcaa gtgctggcag
    11641 aactggcgaa atgtggcatc gagtgggtac agattgatga acccgcgctg gtactggaac
    11701 taccacaggc gtggctggac gcatacaaac ccgcttacga cgcgctccag ggacaggtga
    11761 aactgctgct gaccacctat tttgaaggcg taacgccaaa tctcgacacg attactgcgc
    11821 tgcctgttca ggggctgcat gtggaccttg tacatggtaa agatgacgtt gttgaactgc
    11881 acaagcgcct gccttctgac tggctgctgt ctgcgggtct gatcaatggt cgtaacgtct
    11941 ggcgcgccga tcttactgag aaatatgtgc aaattaagga cattgtcggc aaacgtgatt
    12001 tgtgggtggc atcttcctgc tcgttgctgc acagccccat cgacctgagc gtggaaacgc
    12061 gtcttgatgc agaagtgaaa agctggtttg ccttcgccct acaaaaatgc catgaactgg
    12121 cactgctgcg cgatgcgctg aacagtggtg acacggcagc tctggcagag tggagcgccc
    12181 caattcaggc gcgtcgtcac tctactcgtg tacataatcc ggcggtagaa aagcgtctgg
    12241 cggcgatcac cgcccaggac agccagcgtg cgaatgtcta tgaagtgcgt gctgaagccc
    12301 agcgtgcgcg ttttaaactg ccagcgtggc cgaccaccac gattggttcc ttcccgcaaa
    12361 ccacggaaat tcgtaccctg cgtctggatt tcaaaaaggg caatctcgac gccaataact
    12421 accgcacggg cattgcggaa catatcaggc aggccattgt tgaacaggaa cgtttgggac
    12481 tggatgtgct ggtacatggt gaggccgagc gtaatgacat ggtggaatac tttggcgagc
    12541 acctcgacgg gtttgtcttt acgcaaaacg gttgggtaca gagctacggt tcccgctgcg
    12601 tgaagccacc gattgttatt ggtgacgtta gtcgcccggc accgattacc gtggagtggg
    12661 cgaagtatgc gcaatctatg actgataaac cggtgaaagg gatgctgact ggcccggtga
    12721 ccattctctg ctggtcgttc ccgcgtgaag atgtcagccg tgaaaccatc gccaaacaaa
    12781 ttgcgctggc gctgcgtgat gaagtggcgg acctggaagc cgcaggaatt ggcatcatcc
    12841 agattgatga accggcgctg cgcgaaggtt taccgctgcg tcgtagcgac tgggatgcat
    12901 atctccagtg gggcgtggag gccttccgta tcaacgccgc cgtggcgaaa gatgacacac
    12961 aaatccacac tcacatgtgt tattgcgagt tcaacgacat catggattcg attgcggcac
    13021 tggacgcaga cgtcatcacc atcgaaacct cgcgttctga catggagttg ctggagtcgt
    13081 tcgaagagtt tgattatccg aatgaaatcg gtccaggcgt ctatgacatt cactcgccaa
    13141 acgtaccgag cgtggaatgg attgaagcct tgctgaagaa agcggcaaaa cgcattccgg
    13201 cagagcgcct gtgggtcaac ccggactgtg gcctgaaaac gcgcggctgg ccagaaaccc
    13261 gcgcggcact ggcgaacatg gtgcaggcgg cgcagaactt gcgtcggggg taaaatccaa
    13321 accgggtggt aataccaccc ggtcttttct cattacagcg acttcttccc accatactgc
    13381 ttaaaccatt ccagcatacg ctgccagcca tcttctgcag atgcggcatg atagctcggg
    13441 cgataatcag cgttgaatgc atgcccggcg tccgggtaca cgataatctc tgctttcgca
    13501 ttagcagccc gcagcgcctg gcgcatggtt tcaacgctct cctgcggaat actgttatcc
    13561 agaccgccat ataagccgag aaccggcgcg ttaagatcgg ttgcgatatc aacaggttgt
    13621 ttcggtgaat tcagcgactt atcgcccgtc agtttgccgt accacgccac tgcggctttt
    13681 agctgtggat tatgcgcggc atacagccaa gtgatacgtc caccccagca gaatccggtg
    13741 atcattaaac gatgaacatc gccgccgttg cgggaagccc aactggcgac atgatcgaga
    13801 tcggccagca cctgcgagtc aggcactttt gctaccagac cgctaagcaa cgtggggata
    13861 tcggcaaaat cattcggatc gccttcgcgg aagtaaagtt caggtgcgat agccagatac
    13921 ccctccagcg ccagacggcg acaaatgtcg cggatatgtt catgcacgcc aaaaatttcc
    13981 tgcactacaa tgaccactgg cagtgggcca tcgctttgct ttggtctggc atggtaagca
    14041 ggcatgttat ccccttgtga agggatagag gtgaagcccg ccacaatcgc gtcgtccggg
    14101 gtctgaacga tggtcgaagc gagaggcgat gcagcaggtg caaatccaga ttgttgtgtt
    14161 gttgccatgg tattctccgt acccttataa aaatgttgcg caatgttaac tatagtcagc
    14221 atgcaacaaa tcacattgcc tgaatcggct catcttttat gcagtcctgc agaatgaaga
    14281 gtgatttatg tgatttgcat cacctttggt gggtaaattt atgcaacgca tttgcgtcat
    14341 ggtgatgagt atcacgaaaa aatgttaaac ccttcggtaa agtgtctttt tgcttcttct
    14401 gactaaaccg attcacagag gagttgtata tgtccaagtc tgatgttttt catctcggcc
    14461 tcactaaaaa cgatttacaa ggggctacgc ttgccatcgt ccctggcgac ccggatcgtg
    14521 tggaaaagat cgccgcgctg atggataagc cggttaagct ggcatctcac cgcgaattca
    14581 ctacctggcg tgcagagctg gatggtaaac ctgttatcgt ctgctctacc ggtatcggcg
    14641 gcccgtctac ctctattgct gttgaagagc tggcacagct gggcattcgc accttcctgc
    14701 gtatcggtac aacgggcgct attcagccgc atattaatgt gggtgatgtc ctggttacca
    14761 cggcgtctgt ccgtctggat ggcgcgagcc tgcacttcgc accgctggaa ttcccggctg
    14821 tcgctgattt cgaatgtacg actgcgctgg ttgaagctgc gaaatccatt ggcgcgacaa
    14881 ctcacgttgg cgtgacagct tcttctgata ccttctaccc aggtcaggaa cgttacgata
    14941 cttactctgg tcgcgtagtt cgtcacttta aaggttctat ggaagagtgg caggcgatgg
    15001 gcgtaatgaa ctatgaaatg gaatctgcaa ccctgctgac catgtgtgca agtcagggcc
    15061 tgcgtgccgg tatggtagcg ggtgttatcg ttaaccgcac ccagcaagag atcccgaatg
    15121 ctgagacgat gaaacaaact gaaagccaag cggtgaaaat cgtggtggaa gcggcgcgtc
    15181 gtctgctgta attctcttct cctgtctgaa ggccgacgcg ttcggccttt tgtatttttg
    15241 cgtagcgcct cgcaggaaat gcctttccaa ctggacgttt gtacagcaca attctatttt
    15301 gtgcgggtaa gttgttgcgt caggaggcgt tgtggatttc tcaatcatgg tttacgcagt
    15361 tattgcgttg gtgggtgtgg caattggctg gctgtttgcc agttatcaac atgcgcagca
    15421 aaaagccgag caattagctg aacgtgaaga gatggtcgcg gagttaagcg cggcaaaaca
    15481 acaaattacc caaagcgagc actggcgtgc agagtgcgag ttactcaata acgaagtgcg
    15541 cagcctgcaa agtattaaca cctctctgga ggccgatctg cgtgaagtaa ccacgcggat
    15601 ggaagccgca cagcaacatg ctgacgataa aattcgccag atgattaaca gcgagcagcg
    15661 cctcagtgag cagtttgaaa acctcgccaa ccgtattttt gagcacagca atcgccgggt
    15721 tgatgagcaa aaccgtcaga gtctgaacag cctgttgtcg ccgctacgtg aacaactgga
    15781 cggtttccgc cgtcaggttc aggacagctt cggtaaagaa gcacaagaac gccataccct
    15841 gacccacgaa attcgcaatc tccagcaact caacgcgcaa atggcccagg aagcgatcaa
    15901 cctgacgcgc gcgctgaaag gcgacaataa aacccagggc aactggggcg aggtagtatt
    15961 gacgcgggtg ctggaggctt ccggtctgcg tgaagggtat gaatatgaaa cccaggtcag
    16021 catcgaaaat gacgcccgct cgcggatgca gccggatgtc atcgtgcggc tgccgcaggg
    16081 aaaagatgtg gtgatcgacg ccaaaatgac gctggtcgcc tatgaacgct attttaacgc
    16141 cgaagacgac tacacccgcg aaagcgcgct acaggaacat atcgcgtcgg tgcgtaacca
    16201 tatccgtttg ctgggacgca aagattatca acagctgccg gggctgcgaa ctctggatta
    16261 cgtgctgatg tttattcccg ttgaacccgc ttttttactg gcgcttgacc gccagccgga
    16321 gctgatcacc gaagcgttga aaaacaatat catgctggtt agcccgacta cgctgctggt
    16381 ggcgctgcgc actatcgcca acctgtggcg ttatgagcat caaagccgca acgcccagca
    16441 aatcgccgat cgtgccagca agctgtacga caagatgcgt ttgttcatcg atgacatgtc
    16501 cgcgattggt caaagtctcg acaaagcgca ggataactat cggcaggcaa tgaaaaaact
    16561 ctcttcaggg cgcggaaatg tgctggcgca ggcagaagcg tttcgcggtt taggagtaga
    16621 aattaaacgc gagattaatc cggatttggc tgaacaggcg gtgagccagg atgaagagta
    16681 tcgacttcgg tcggttccgg agcagccgaa tgatgaagct tatcaacgcg atgatgaata
    16741 taatcagcag tcgcgctagc ccattgggag tagttaagcc gggtagaaat ctagggcatc
    16801 gacgcccaat ctgttacact tctggaacaa ttttttgatg agcaggcatt gagatggtgg
    16861 ataagtcaca agaaacgacg cactttggtt ttcagaccgt cgcgaaggaa caaaaagcgg
    16921 atatggtcgc ccacgttttc cattccgtgg catcaaaata cgatgtcatg aatgatttga
    16981 tgtcatttgg tattcatcgt ttgtggaagc gattcacgat tgattgcagc ggcgtacgcc
    17041 gtgggcagac cgtgctggat ctggctggtg gcaccggcga cctgacagcg aaattctccc
    17101 gcctggtcgg agaaactggc aaagtggtcc ttgctgatat caatgaatcc atgctcaaaa
    17161 tgggccgcga gaagctgcgt aatatcggtg tgattggcaa cgttgagtat gttcaggcga
    17221 acgctgaggc gctgccgttc ccggataaca cctttgattg catcaccatt tcgtttggtc
    17281 tgcgtaacgt caccgacaaa gataaagcac tgcgttcaat gtatcgcgtg ctgaaacccg
    17341 gcggccgcct gctggtgctt gagttctcga agccaattat cgagccgctg agcaaagcct
    17401 atgatgcata ctccttccat gtgctgccgc gtattggctc actggtcgcg aacgacgccg
    17461 acagctaccg ttatctggca gaatccatcc gtatgcatcc cgatcaggat accctgaaag
    17521 ccatgatgca ggatgccgga ttcgaaagtg tcgactacta caatctgacg gcaggggttg
    17581 tggcgctgca tcgtggttat aagttctgac aggagaccgg aaatgccttt taaaccttta
    17641 gtgacggcag gaattgaaag tctgctcaac accttcctgt atcgctcacc cgcgctgaaa
    17701 acggcccgct cgcgtctgtt gggcaaagtg ctgcgcgtgg aggtaaaagg cttttcgacg
    17761 tcattgattc tggtgttcag cgaacgccag gttgatgtac tgggcgaatg ggcaggcgat
    17821 gctgactgca ccgttatcgc ctacgccagt gtgttgccga aacttcgcga tcgccagcag
    17881 cttgccgccc tgattcgcag tggtgagctg gaagtgcagg gcgatattca ggtggtgcaa
    17941 aacttcgttg cgctggcagg tctggcagag ttcgaccctg cggaactgct ggccccttat
    18001 accggtgata tcgccgctga aggaatcagc aaagccatgc gcggaggcgc aaagttcctg
    18061 catcacggca ttaagcgcca gcaacgttat gtggcggaag ccattactga agagtggcgt
    18121 atggcacccg gtccgcttga agtggcctgg tttgcggaag agacggctgc cgtcgagcgt
    18181 gctgttgatg ccctgatcaa acggctggaa aaactggagg ctaaatgacg ccaggtgaag
    18241 tacggcgcct atatttcatc attcgcactt ttttaagcta cggacttgat gaactgatcc
    18301 ccaaaatgcg tatcaccctg ccgctacggc tatggcgata ctcattattc tggatgccaa
    18361 atcggcataa agacaaactt ttaggtgagc gactacgact ggccctgcaa gaactggggc
    18421 cggtatggat caagttcggg caaatgttat caacccgccg cgatcttttt ccgccgcata
    18481 ttgccgatca gctggcgtta ttgcaggaca aagttgctcc gtttgatggc aagctggcga
    18541 agcagcagat tgaagctgca atgggcggct tgccggtaga agcgtggttt gacgattttg
    18601 aaatcaagcc actggcttct gcttctatcg cccaggttca taccgcgcga ttgaaatcga
    18661 atggtaaaga ggtggtgatt aaagtcatcc gcccggatat tttgccggtc attaaagcag
    18721 acctgaaact tatctaccgt ctggctcgct gggtgccgcg tttgctgccg gatggtcgcc
    18781 gtctacgccc aaccgaagtg gtgcgcgagt acgaaaagac cttgattgat gaactgaatt
    18841 tgctgcggga atctgccaac gccattcagc ttcggcgaaa ttttgaagac agcccgatgc
    18901 tctacatccc ggaggtttac cctgactatt gtagtgaagg gatgatggtg atggagcgta
    18961 tttacggcat tccggtgtct gatgttgcgg cgctggagaa aaacggcacc aacatgaaat
    19021 tgctggcgga acgcggcgtg caggtgttct tcactcaggt ctttcgcgac agctttttcc
    19081 atgccgatat gcaccctggc aacatcttcg taagctatga acacccggaa aacccgaaat
    19141 atatcggcat tgattgcggg attgttggct cgctaaacaa agaagataaa cgctatctgg
    19201 cagaaaactt tattgccttc tttaatcgcg actatcgcaa agtggcagag ctacacgtcg
    19261 attctggctg ggtgccacca gataccaacg ttgaagagtt cgaatttgct attcgtacgg
    19321 tctgtgaacc tatctttgag aaaccgctgg ccgaaatttc gtttggacat gtactgttaa
    19381 atctgtttaa tacggcgcgt cgcttcaata tggaagtgca gccgcaactg gtgttactcc
    19441 agaaaaccct gctctacgtc gaaggggtag gacgccagct ttatccgcaa ctcgatttat
    19501 ggaaaacggc gaagcctttc ctggagtcgt ggattaaaga tcaggtcggt attcctgcgc
    19561 tggtgagagc atttaaagaa aaagcgccgt tctgggtcga aaaaatgcca gaactgcctg
    19621 aattggttta cgacagtttg cgccagggca agtatttaca gcacagtgtt gataagattg
    19681 cccgcgagct tcagtcaaat catgtacgtc agggacaatc gcgttatttt ctcggaattg
    19741 gcgctacgtt agtattaagt ggcacattct tgttggtcag ccgacctgaa tgggggctga
    19801 tgcccggctg gttaatggca ggtggtctga tcgcctggtt tgtcggttgg cgcaaaacac
    19861 gctgattttt tcatcgctca aggcgggccg tgtaacgtat aatgcggctt tgtttaatca
    19921 tcatctacca cagaggaaca tgtatgggtg gtatcagtat ttggcagtta ttgattattg
    19981 ccgtcatcgt tgtactgctt tttggcacca aaaagctcgg ctccatcggt tccgatcttg
    20041 gtgcgtcgat caaaggcttt aaaaaagcaa tgagcgatga tgaaccaaag caggataaaa
    20101 ccagtcagga tgctgatttt actgcgaaaa ctatcgccga taagcaggcg gatacgaatc
    20161 aggaacaggc taaaacagaa gacgcgaagc gccacgataa agagcaggtg taatccgtgt
    20221 ttgatatcgg ttttagcgaa ctgctattgg tgttcatcat cggcctcgtc gttctggggc
    20281 cgcaacgact gcctgtggcg gtaaaaacgg tagcgggctg gattcgcgcg ttgcgttcac
    20341 tggcgacaac ggtgcagaac gaactgaccc aggagttaaa actccaggag tttcaggaca
    20401 gtctgaaaaa ggttgaaaag gcgagcctca ctaacctgac gcccgaactg aaagcgtcga
    20461 tggatgaact acgccaggcc gcggagtcga tgaagcgttc ctacgttgca aacgatcctg
    20521 aaaaggcgag cgatgaagcg cacaccatcc ataacccggt ggtgaaagat aatgaagctg
    20581 cgcatgaggg tgtaacgcct gccgctgcac aaacgcaggc cagttcgccg gaacagaagc
    20641 cagaaaccac gccagagccg gtggtaaaac ctgctgcgga cgctgaaccg aaaaccgctg
    20701 caccttcccc ttcgtcgagt gataaaccgt aaacatgtct gtagaagata ctcaaccgct
    20761 tatcacgcat ctgattgagc tgcgtaagcg tctgctgaac tgcattatcg cggtgatcgt
    20821 gatattcctg tgtctggtct atttcgccaa tgacatctat cacctggtat ccgcgccatt
    20881 gatcaagcag ttgccgcaag gttcaacgat gatcgccacc gacgtggcct cgccgttctt
    20941 tacgccgatc aagctgacct ttatggtgtc gctgattctg tcagcgccgg tgattctcta
    21001 tcaggtgtgg gcatttatcg ccccagcgct gtataagcat gaacgtcgcc tggtggtgcc
    21061 gctgctggtt tccagctctc tgctatttta tatcggcatg gcgttcgcct actttgtggt
    21121 ctttccgctg gcatttggct tccttgccaa taccgcgccg gaaggggtgc aggtatccac
    21181 cgacatcgcc agctatttaa gcttcgttat ggcgctgttt atggcgtttg gtgtctcctt
    21241 tgaagtgccg gtggcaattg tgctgctgtg ctggatgggg attacctcgc cagaagactt
    21301 acgcaaaaaa cgcccgtatg tgctggttgg tgcattcgtt gtcgggatgt tgctgacgcc
    21361 gccggatgtc ttctcgcaaa cgctgttggc gatcccgatg tactgtctgt ttgaaatcgg
    21421 tgtcttcttc tcacgctttt acgttggtaa agggcgaaat cgggaagagg aaaacgacgc
    21481 tgaagcagaa agcgaaaaaa ctgaagaata aattcaaccg cccgtcaggg cggttgtcat
    21541 atggagtaca ggatgtttga tatcggcgtt aatttgacca gttcgcaatt tgcgaaagac
    21601 cgtgatgatg ttgtagcgcg cgcttttgac gcgggagtta atgggctact catcaccggc
    21661 actaacctgc gtgaaagcca gcaggcgcaa aagctggcgc gtcagtattc gtcctgttgg
    21721 tcaacggcgg gcgtacatcc tcacgacagc agccagtggc aagctgcgac tgaagaagcg
    21781 attattgagc tggccgcgca gccagaagtg gtggcgattg gtgaatgtgg tctcgacttt
    21841 aaccgcaact tttcgacgcc ggaagagcag gaacgcgctt ttgttgccca gctacgcatt
    21901 gccgcagatt taaacatgcc ggtatttatg cactgtcgcg atgcccacga gcggtttatg
    21961 acattactgg agccgtggct ggataaactg cctggtgcgg ttcttcattg ctttaccggc
    22021 acacgcgaag agatgcaggc gtgcgtggcg catggaattt atatcggcat taccggttgg
    22081 gtttgcgatg aacgacgcgg actggagctg cgggaacttt tgccgttgat tccggcggaa
    22141 aaattactga tcgaaactga tgcgccgtat ctgctccctc gcgatctcac gccaaagcca
    22201 tcatcccggc gcaacgagcc agcccatctg ccccatattt tgcaacgtat tgcgcactgg
    22261 cgtggagaag atgccgcatg gctggctgcc accacggatg ctaatgtcaa aacactgttt
    22321 gggattgcgt tttagagttt gcggaactcg gtgttcttca cactgtgctt aatctcttta
    22381 ttaataagat taagcaatag catggagcga gcctcaccat cgggttcggt gaaaatggcc
    22441 tgaaagcctt cgaacgcgcc ttcggtaata atcaccttat cacccggata aggggttgcc
    22501 ggatcgacaa tgtctttcgg tttatatacc gatagctgat gaatcaccgc cgatgggact
    22561 atcgctggcg acgcgccaaa gcgcacgaag tggctgacac cgcgggtcgc gttgatagtc
    22621 gtggtatgaa tcacttctgg atcaaattcc acaaacaggt agttggggaa caatggctca
    22681 ctgactgcag tacgtttgcc acgcacgatt ttttccaggg tgatcatcgg tgccaggcaa
    22741 ttcacagcct gtctttcgag gtgttcctgg gcacgttgaa gttgcccgcg cttgcagtac
    22801 agtaaatacc aggattgcat aatgactctt atccgtttaa tcggggcgca aggatagcaa
    22861 aagctttacg ctaagttaat tatattcccc ggtttgcgtt ataccgtcag agttcacgct
    22921 aatttaacaa atttacagca tcgcaaagat gaacgccgta taatgggcgc agattaagag
    22981 gctacaatgg acgccatgaa atataacgat ttacgcgact tcttgacgtt gcttgaacag
    23041 cagggtgagc taaaacgtat cacgctcccg gtggacccgc atctggaaat cactgaaatt
    23101 gctgaccgca cgctgcgtgc tggtgggcct gcgctgttgt ttgaaaaccc taaagggtac
    23161 tcaatgccgg tgctgtgcaa cttgttcggt acgccaaagc gcgtagcgat gggtatgggc
    23221 caggaagatg tttcagcact gcgtgaagtc ggtaaattat tagcatttct gaaagaacca
    23281 gagccgccaa aaggttttcg cgatctgttt gataagctgc cgcagtttaa gcaggtgtta
    23341 aacatgccga caaagcgact gcgcggtgca ccctgccaac aaaaaatcgt ctctggcgat
    23401 gacgtcgatc tcaaccgtat tcccattatg acctgttggc cggaagatgc cgcgccgctg
    23461 attacatggg ggctaaccgt tacacgtggc cctcataaag agcgacagaa tctgggcatt
    23521 tatcgccagc aactgattgg taaaaacaag ctgattatgc gttggctgtc gcatcgcggc
    23581 ggcgcgctgg attatcagga gtggtgtgcg gcgcatccag gtgaacgttt cccgatctct
    23641 gtggcgttgg gcgctgatcc ggcaaccatt ctcggtgcag tcacaccagt accagatact
    23701 ttgtcggaat acgcctttgc cggattgcta cgtggcacca aaaccgaagt agtgaagtgt
    23761 atttccaatg atctcgaagt gcccgccagt gcggagattg tgctggaagg gtatatcgaa
    23821 caaggcgaaa tggcgccaga aggaccgtat ggtgaccaca ctggttacta taacgaagtc
    23881 gatagtttcc cggtatttac cgtgacgcat attacccagc gtgaagatgc gatttaccat
    23941 tccacctata ccgggcgtcc gccagatgaa cccgcggtac tgggagtggc gttgaacgaa
    24001 gtatttgttc ccattctgca aaagcagttc ccggaaattg tcgattttta cctgccgccg
    24061 gaaggctgct cttatcgcct ggcggtagtg acaatcaaaa aacagtacgc cggacacgcg
    24121 aagcgcgtca tgatgggcgt ctggtcgttc ttacgccagt ttatgtacac taaatttgtg
    24181 atcgtttgcg atgatgacgt taacgcacgc gactggaacg atgtgatttg ggcgattacc
    24241 acccgtatgg acccagcgcg ggatactgtt ctggtagaaa atacgcctat tgattatctg
    24301 gattttgcct cgcctgtctc cgggctgggt tcaaaaatgg ggctggatgc cacgaataaa
    24361 tggccggggg aaacccagcg tgaatgggga cgtcccatca aaaaagatcc agatgttgtc
    24421 gcacatattg acgccatctg ggatgaactg gctattttta acaacggtaa aagcgcctga
    24481 tgcgcgtttg ttttgcccta tttatcgatc cgacagagaa agcgcatgac aactttaagc
    24541 tgtaaagtga cctcggtaga agctatcacg gataccgtat atcgtgtccg catcgtgcca
    24601 gacgcggcct tttcttttcg tgctggtcag tatttgatgg tagtgatgga tgagcgcgac
    24661 aaacgtccgt tctcaatggc ctcgacgccg gatgaaaaag ggtttatcga gctgcatatt
    24721 ggcgcttctg aaatcaacct ttacgcgaaa gcagtcatgg accgcatcct caaagatcat
    24781 caaatcgtgg tcgatattcc ccacggagaa gcgtggctgc gcgatgatga agagcgtccg
    24841 atgattttga ttgcgggcgg caccgggttc tcttatgccc gctcgatttt gctgacagcg
    24901 ttggcgcgta acccaaaccg tgatatcacc atttactggg gcgggcgtga agagcagcat
    24961 ctgtatgatc tctgcgagct tgaggcgctt tcgttgaagc atcctggtct gcaagtggtg
    25021 ccggtggttg aacaaccgga agcgggctgg cgtgggcgta ctggcaccgt gttaacggcg
    25081 gtattgcagg atcacggtac gctggcagag catgatatct atattgccgg acgttttgag
    25141 atggcgaaaa ttgcccgtga cctgttttgc agtgagcgta atgcgcggga agatcgcctg
    25201 tttggcgatg cgtttgcatt tatctgagat ataaaaaaac ccgcccctga caggcgggaa
    25261 gaacggcaac taaaacttta ttcagtggca tttagatcta tgtcgcatcc ggcattcggc
    25321 acacgatgcc tgatgcgacg ctggcgcgtc ttatcaggcc tacattggtg ccggatcggt
    25381 agaccggata aggcgttcac gccgcatccg gcaagtggtt aaacccgctc aaacaccgtc
    25441 gcaataccct gacccagacc gatacacatc gtcgccagac caaactgaac gtctttgtgt
    25501 tccatcagat tcagcagcgt ggtgctgata cgcgcaccgg aacaacccag cggatgaccc
    25561 agcgcgatcg cgccaccgtt gaggttgatc ttctcgtcaa tctgctcaat tagtcccaga
    25621 tctttaatac atggcaggat ctgcgcggca aaggcttcat tcatttcaaa cacgccgata
    25681 tcactgacag aaagccccgc ttttttcagc gccagcttcg aggccggaac cgggccgtaa
    25741 cccatgatcg atgggtcaca accaacgacc gccatcgaac gcacacgagc gcgcggctta
    25801 agacctaatt catgggcgcg gctttcactc atcaccagca tggcagctgc gccatcggaa
    25861 agtgcagaag atgtgcccgc cgttaccgta ccgtttactg gatcaaacgc cggacgcagc
    25921 gtggcgaggg cttccacggt ggtttccggg cgaatcactt cgtcgtaatt aaactgcttc
    25981 aggacgccgt cggcatcgtg accaccagtt gggatgattt catttttaaa tgcgcccgac
    26041 tgcgtggcgg cccaggcgcg ggcgtgtgac cgcgcggcaa aggcatcctg catttcacgg
    26101 ctgataccgt gcatacgcgc cagcatttct gccgttaagc ccatcatgcc cgccgctttg
    26161 gcgacattgc ggctcaggcc agggtgaaaa tcgacgccgt gactcatcgg cacatggccc
    26221 atatgctcca cgccgccaac cagacatgcc tgcgcatcgc cggtcatgat cattcgtgct
    26281 gcgtcatgca gtgcctgcat ggatgaacca cacaagcgat tgacagtaac cgccgggaca
    26341 gagtgcggca cttctgccag cagcgccgcg ttacgggcga tattaaaacc ctgctccagc
    26401 gtctgctgca cacaccccca gtaaatatcg tcgagggccg ccgcttccag cgccgggtta
    26461 cgcgccagca ggctacgcat taaatgagcg gagagatctt ctgcacgcac gttacgaaaa
    26521 gcaccgccct tcgaacggcc catcggggtg cgaattgcat cgacaatgac aacctgttcc
    26581 attgtgactc cttaagccgt tttcaggtcg ccaaccggac gggctggctc aaccggggga
    26641 tagtacggtt cgttatgacg cgctttatta cgcagacctt ccggcacttc gtacagcggg
    26701 ccgaggtgct gatattgctg tgccatatcg aggtattttg cgctgccgag ggtgtccagc
    26761 cagcggaacg cgccgccgtg gaacggaggg aagcccaggc cgtagaccag cgccatatcc
    26821 gcttctgccg gagtggcgat aatgccttct tccagacagc gcaccacttc gttgaccatc
    26881 gggatcatca tgcgggcgat aatctcttct tcgctgaaat cgcgcttcgg ctggctcact
    26941 tctgccagca ggtcgtcaac agcggcgtct tcttctttct tcggcttacc tttgctgtct
    27001 tctttataat gccagaaacc gaggccgttc ttctgaccaa agcggttggc atcaaacagc
    27061 gcgtcgatgg catcgcgata atctttctgc atccgctgtg ggaagcccgc tgccatgact
    27121 gcctgggcgt gatgcgcggt gtcaatgccc acaacgtcca gcagatatgc cggacccata
    27181 ggccagccaa actgtttttc catcactttg tcgatcttgc ggaaatccgc gccgtcgcgc
    27241 agcaactggc tgaaaccggc gaaatacggg aacagcacgc ggttgacgaa gaagccgggg
    27301 caatcgttaa ccacaatcgg cgttttaccc atcttgctcg cccaggcgac gactttcgca
    27361 atagtttcgt cggagctttt ctcgccgcga ataatttcca ccaacggcat tcggtggacc
    27421 gggttaaaga agtgcatccc gcagaagttt tccggacgtt ccagcgcgtt ggccagttcg
    27481 ctgataggaa tggttgaagt gttagacgcc agcacggtat ccgggcgtac tttctgctcg
    27541 gtttctgcca gcacggcttt tttcactttc gggttttcaa caatcgcttc taccacaaca
    27601 tccacgcgct caaatccggc gtagtcgagc gttgggtgga tcgtggagat cacgccagcc
    27661 agtttcagac catcgatctt gccgcgctca agctgcttgt tcagtagttt cgcggcttca
    27721 gtcatgccga gggctaacga cttgtcgtta atatctttca taatcaccgg cacgcctttc
    27781 cacgcagact ggtaagcgat gccgccaccc ataatgcctg cgcccagcac ggcggcctgt
    27841 ttcggggttt caacgtcttt ggtgagtttc ttcgctttgc cttttacata ttgatcgtta
    27901 aggaaaatgc cgaccagtgc gcgagcttcg ttggtatgcg ccagcgggac aaagcttttg
    27961 ttttctaggt ttaaggcttc ttcacgacca aaacgagccg cagcttcaat ggtttttact
    28021 gcggtgatgg gggccgggta atgttttcct gccgtttgtg cgaccatccc tttagcgatg
    28081 gtgaagctca tggtggcttc aatcttgctc agttttagcg gttccagctt cggctgacgt
    28141 ttcgctttcc agtcgaggtc gccgttaatg gcctggcgta aaatcgccat cgccccttca
    28201 atcagttttt ctgctttgac tacgccatcc accagaccga ttttcagcgc ctgatccgcg
    28261 ccgacatctt taccggcggc aatgatttcc agcgcactgt cagcgcccag catacgtggc
    28321 atacgtacag aaccgccaaa gcccggcatg atgcccagtt tggtttccgg cagaccgatg
    28381 cgcagatccg gcgtcgccag acggtaatcg gtcgccagca cgcattcgca gccaccgccc
    28441 agcgcatagc cattgacggc agcaatggtc ggcaccggca gatcttccag gcgattaaac
    28501 acgctattgg caaagtgcag ccactgactt aactgttctt caggaacgag gaacagggac
    28561 aaaaattcgg tgatatcagc accgacgata aaggctgctt tgttcgaacg cagcagcagc
    28621 ccttttagat ctgattgctg ttccagcacg ccgatggcct cgccgaggct ggcgacggtc
    28681 gcagtgtcga gtttattaac tgaacctggg gcatcaaata ccagttcggc aatgccatct
    28741 tccagccagt caaggtacag ggtgtcgcct ttgtaaagca tgtcagtctc ctgaatccgc
    28801 aaggtgatct ggtcgtacca gatgcatcga agtgtgtatt ttgtgttaaa aatatgcaaa
    28861 taaaagatta aagaaatgcc gatctgatca cgctcggcag aaatcacgct ctggatgaac
    28921 gatgtgctaa gatgcggaga cttaaggtca aaaaaacaga agggtaaaaa atggaatcac
    28981 tggcctcgct ctataaaaat catatagcta cattgcagga acgaactcgc gatgcgctga
    29041 cgcgcttcaa gctggatgcg ttacttattc actccggcga actgttcaac gtttttctcg
    29101 acgatcatcc ctatccgttt aaagtgaacc cgcaattcaa agcgtgggtg ccggtaactc
    29161 aggtgccaaa ctgctggctg ctggtggatg gcgtgaataa gccgaaactg tggttctatc
    29221 tgccggttga ttactggcac aacgtcgaac cgctgccgac ctccttctgg actgaagatg
    29281 tagaagtgat cgcgctgccg aaagccgatg gcattggtag tctgttgcct gctgcgcgcg
    29341 gcaatatcgg ttatatcggt ccggtgccgg aacgtgcgct gcaactgggt attgaggcca
    29401 gcaatatcaa cccgaaaggg gttatcgact acctgcatta ctaccgctcc ttcaaaaccg
    29461 agtacgagct ggcctgtatg cgtgaagcgc agaaaatggc ggtcaacggt catcgcgcgg
    29521 cagaagaagc gttccgttct ggcatgagcg agttcgatat caatattgcc tatctgactg
    29581 cgaccggtca tcgtgatacc gacgtacctt acagcaacat tgtggcactc aacgaacacg
    29641 ctgcggtgct gcattacacc aaactggatc atcaggcgtc ggaagagatg cgcagcttcc
    29701 tgctggatgc cggggccgaa tataacggct atgccgctga cctgacccgt acctggtcgg
    29761 caaaaagtga caacgattac gcacagctgg tgaaagacgt aaatgatgaa caactggcgc
    29821 tgatcgcgac catgaaagct ggcgttagct atgtggatta ccacatccag ttccatcagc
    29881 gcatcgccaa attgctgcgt aaacatcaaa tcatcaccga tatgagtgaa gaggcgatgg
    29941 tcgaaaacga tcttaccggg ccgtttatgc cgcatggtat cggccatccg ctgggcctgc
    30001 aggtgcatga cgtcgccggt tttatgcagg atgatagcgg tacgcacctc gcggcaccgg
    30061 caaaatatcc gtacctgcgc tgcacccgta ttctccagcc gggcatggtg ttaaccatcg
    30121 aaccgggtat ctacttcatt gaatcgctgc tggcaccgtg gcgtgaaggg cagttcagca
    30181 agcacttcaa ctggcagaaa attgaagcac tgaaaccgtt cggcggcatt cgtatcgaag
    30241 acaacgtggt gatccacgaa aataacgtgg aaaacatgac ccgggatctg aaactggcgt
    30301 gatggaaagc tggttaattc ctgcggcacc ggtcacggtc gttgaagaga tcaaaaaaag
    30361 ccgcttcatt acgctgttgg cgcataccga tggcgttgag gcggcgaaag cgtttgttga
    30421 atcggtgcgg gcagaacacc ccgatgcccg ccaccattgc gtggcgtggg tggcgggtgc
    30481 gccggatgat tctcaacagc tgggtttctc tgacgacggg gagccggcgg gaacggcagg
    30541 taaaccgatg ctcgcccagc taatgggcag cggcgttggg gaaattaccg ctgtggtggt
    30601 gcgctactac ggcggcatat tgcttggcac cggagggtta gtgaaagcgt atggcggcgg
    30661 cgtgaatcag gcgctgcgcc agctaacgac ccaacgcaag acgccattaa ccgaatatac
    30721 tttgcaatgt gaatatagtc agttaaccgg cattgaagcg ttgctggggc agtgtgacgg
    30781 caaaattatc aacagtgatt atcaggcatt cgttctgctg cgggtggcgc ttccggcggc
    30841 gaaagtggct gaattttccg cgaagctggc ggattttagc cgtggttcat tgcaattgtt
    30901 agcgattgaa gaataatccc cacttcgttt tgcagaacta aggaagcggc agagatgcat
    30961 tttcgcgcca ttacccgaat cgttggacta ctggtcatct tattttcagg gaccatgatt
    31021 atccctgggc tggtagcact catctaccgg gatggagcgg gccgcgcttt tacccagacc
    31081 ttttttgtcg ccctcgccat tggctctatg ctgtggtggc cgaaccgcaa agagaagggc
    31141 gaactgaaat cccgtgaggg gtttctgata gtggtgctgt tctggaccgt gctgggtagt
    31201 gtcggtgcgc tcccttttat cttctcggaa agcccgaacc tcacgattac cgatgcgttt
    31261 tttgaatctt tctctggcct gaccaccacc ggggccacta cgctggtggg gctggattcg
    31321 ctccctcatg ccatcctctt ttatcgccag atgctgcaat ggtttggcgg gatggggatc
    31381 atcgtgttag cggttgcgat actgcctatc ctcggcgtgg gtgggatgca gctctatcgc
    31441 gcagaaatgc ccggcccgct gaaagataac aaaatgcgcc cgcgaattgc ggaaacggcg
    31501 aaaaccctgt ggttgattta tgtcttgctg accgtcgcct gtgcgctggc gttgtggttt
    31561 gccggaatgg atgcctttga tgccatcggc catagctttg cgactatcgc tattggcggc
    31621 ttctcgacac atgatgccag tatcggttat ttcgatagcc cgactattaa cactatcatt
    31681 gctatcttcc tgctgatctc cggctgtaac tacggtctgc acttttcact gttaagtggg
    31741 cgtagtctga aggtttattg gcgcgatccg gaatttcgca tgtttatcgg cgtacagttt
    31801 acgctggtgg ttatttgtac cctcgtactg tggtttcata atgtctacag ttcggcgctg
    31861 atgacaatta accaggcgtt tttccaggtg gtgtcgatgg cgacaaccgc cgggtttacg
    31921 actgacagca ttgcccgctg gccgctcttt ttgccggtac tgcttttatg ttcagctttt
    31981 atcggcggtt gtgccgggtc aacgggcggt ggcctgaaag tgatccgcat cctgctgctg
    32041 tttaagcagg ggaaccgtga actgaaacga ctggtgcatc cgaacgccgt ctatagcatt
    32101 aagctgggga atcgcgcact gccggaacgt atcctcgaag ccgtttgggg atttttctcc
    32161 gcctatgcat tggtgtttat tgtcagtatg ctggcgatta tcgccacggg cgtggatgac
    32221 ttttctgcct ttgcctcggt tgttgcgaca ttgaataacc tggggccagg gcttggcgtg
    32281 gttgctgata actttaccag tatgaacccg gtggctaaat ggatcctgat tgccaacatg
    32341 ctgtttggtc gtctcgaggt ctttacattg ctggtgctct ttaccccgac tttctggcgt
    32401 gaatgatgga gtaatacgtg aaaacattaa ttcttttctc aacaagggac ggacaaacgc
    32461 gcgagattgc ctcctacttg gcttcggaac tgaaagaact ggggatccag gcggatgtcg
    32521 ccaatgtgca ccgcattgaa gaaccacagt gggaaaacta cgaccgtgtg gtcattggtg
    32581 cttctattcg ctatggtcac taccactctg cgttccagga atttgtcaaa aagcatgcga
    32641 cgcggctgaa ttcgatgccg agcgcgtttt actccgtaaa cctggtggcg cgtaaaccgg
    32701 agaagcgcac tccacagacc aacagctacg cgcggaagtt tctgatgaac tcgcaatggc
    32761 gtcccgatcg ctgcgcggtc attgccgggg cgctgcgtta cccacgttat cgctggtacg
    32821 accgttttat gatcaagctg attatgaaga tgtcaggcgg tgaaacggat acgcgcaaag
    32881 aagttgtcta taccgattgg gagcaggtgg cgaatttcgc ccgagaaatc gcccatttaa
    32941 ccgacaaacc gacgctgaaa taagcataaa gaataaaaaa tgcgcggtca gaaaattatt
    33001 ttaaatttcc ccttgtcagg ccggaataac tccctataat gcgccaccac tgacacggaa
    33061 caacggcaaa cacgccgccg ggtcagcggg attctcctga gaattccg
//
LOCUS       DAZFAB010000043        33038 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZFAB010000043 DAZFAB010000000
VERSION     DAZFAB010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33038
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.043"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            92..1777
                     /locus_tag="ACHJWA_002363"
     CDS             92..1777
                     /locus_tag="ACHJWA_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFO4308950.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGMLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELSDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVADASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISAVL"
     gene            1815..4187
                     /locus_tag="ACHJWA_002364"
     CDS             1815..4187
                     /locus_tag="ACHJWA_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFO4308951.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKFKRFNADDSSVKLGYRTTRSDWLTSHINENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGMAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGNVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADRISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHISQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            4244..7027
                     /locus_tag="ACHJWA_002365"
     CDS             4244..7027
                     /locus_tag="ACHJWA_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO4308952.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLLQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMEELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNEVAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLTKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            7389..8789
                     /locus_tag="ACHJWA_002366"
     CDS             7389..8789
                     /locus_tag="ACHJWA_002366"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFO4308953.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSD
                     HPKLQGIAQQNSFKHT"
     gene            8945..10480
                     /gene="gadC"
                     /locus_tag="ACHJWA_002367"
     CDS             8945..10480
                     /gene="gadC"
                     /locus_tag="ACHJWA_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFO4308954.1"
                     /translation="MATLVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            10611..11930
                     /locus_tag="ACHJWA_002368"
     CDS             10611..11930
                     /locus_tag="ACHJWA_002368"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFO4308955.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            12307..13689
                     /gene="dosC"
                     /locus_tag="ACHJWA_002369"
     CDS             12307..13689
                     /gene="dosC"
                     /locus_tag="ACHJWA_002369"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HFO4308956.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPPIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            13714..16103
                     /gene="dosP"
                     /locus_tag="ACHJWA_002370"
                     /gene_synonym="pdeO"
                     /pseudo
     CDS             13714..16103
                     /gene="dosP"
                     /locus_tag="ACHJWA_002370"
                     /gene_synonym="pdeO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
     gene            16361..16942
                     /gene="ddpX"
                     /locus_tag="ACHJWA_002371"
     CDS             16361..16942
                     /gene="ddpX"
                     /locus_tag="ACHJWA_002371"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFO4308957.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFTCFISPGTQHVS"
     gene            16956..18506
                     /locus_tag="ACHJWA_002372"
     CDS             16956..18506
                     /locus_tag="ACHJWA_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO4308958.1"
                     /translation="MKRSISFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASNDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            18508..19530
                     /locus_tag="ACHJWA_002373"
     CDS             18508..19530
                     /locus_tag="ACHJWA_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4308959.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPRGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            19527..20423
                     /gene="ddpC"
                     /locus_tag="ACHJWA_002374"
     CDS             19527..20423
                     /gene="ddpC"
                     /locus_tag="ACHJWA_002374"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFO4308960.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            20420..21406
                     /locus_tag="ACHJWA_002375"
     CDS             20420..21406
                     /locus_tag="ACHJWA_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO4308961.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDTVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARDSGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            21399..22325
                     /locus_tag="ACHJWA_002376"
     CDS             21399..22325
                     /locus_tag="ACHJWA_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFO4308962.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMMFQDPLSSLNPRLPVWRI
                     ITEPLWIAKHSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGEAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     RERCPLATSGCEVRQSLTTRADGREIRCWRAL"
     gene            complement(22381..22812)
                     /gene="osmC"
                     /locus_tag="ACHJWA_002377"
     CDS             complement(22381..22812)
                     /gene="osmC"
                     /locus_tag="ACHJWA_002377"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFO4308963.1"
                     /translation="MTIHKKGQAHWESDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            23157..23372
                     /gene="bdm"
                     /locus_tag="ACHJWA_002378"
     CDS             23157..23372
                     /gene="bdm"
                     /locus_tag="ACHJWA_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFO4308964.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            23474..23611
                     /gene="sra"
                     /locus_tag="ACHJWA_002379"
     CDS             23474..23611
                     /gene="sra"
                     /locus_tag="ACHJWA_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFO4308965.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            23768..25465
                     /gene="maeA"
                     /locus_tag="ACHJWA_002380"
     CDS             23768..25465
                     /gene="maeA"
                     /locus_tag="ACHJWA_002380"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO4308966.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDRDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            25599..26609
                     /gene="adhP"
                     /locus_tag="ACHJWA_002381"
     CDS             25599..26609
                     /gene="adhP"
                     /locus_tag="ACHJWA_002381"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFO4308967.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            26755..27039
                     /locus_tag="ACHJWA_002382"
     CDS             26755..27039
                     /locus_tag="ACHJWA_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO4308968.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAAKTVDVSRLRRLVAQ"
     gene            complement(27267..27920)
                     /gene="fdnI"
                     /locus_tag="ACHJWA_002383"
     CDS             complement(27267..27920)
                     /gene="fdnI"
                     /locus_tag="ACHJWA_002383"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFO4308969.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(27913..28797)
                     /gene="fdnH"
                     /locus_tag="ACHJWA_002384"
     CDS             complement(27913..28797)
                     /gene="fdnH"
                     /locus_tag="ACHJWA_002384"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFO4308970.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQSGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(28810..31857)
                     /gene="fdnG"
                     /locus_tag="ACHJWA_002385"
     CDS             complement(28810..31857)
                     /gene="fdnG"
                     /locus_tag="ACHJWA_002385"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(31270..31272),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO4308971.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKEHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLAGQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            32089..32970
                     /gene="yddG"
                     /locus_tag="ACHJWA_002386"
     CDS             32089..32970
                     /gene="yddG"
                     /locus_tag="ACHJWA_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFO4308972.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         8406 a         8189 c         8175 g         8268 t
ORIGIN      
        1 atatttcata ttgttaaatg atacttaaat aaatttcatc agtaaatcat tgccatagat
       61 aatcattgga tggcacaatc aattttcgct aatgataacc attcccatta cgttttgtat
      121 gttgatagca aagtatctct gtctgctgaa gcccttttgg ctgcgtaaga ataataaaac
      181 ttcagtgttg ttaatcatta ttatcctcgc catgatcctt ggcgtggtta aaattcaggt
      241 ctggttaaac gactggaata atgatttttt caatgcgcta agccagaaag aaaccgataa
      301 gctttggcag cttgtccttt ggttccctgc cctgctgggg atctttgtat taatatccgt
      361 aaacaaaaca tggttaatta aactactgac tattcgctgg cgcgaatggt taacagatta
      421 ctaccttaat cgctggtttg cagataaaaa ctactacttc acgcagatct acggcgagca
      481 taaaaataca gataaccctg accaacgtat cgctgaagac attctcttac tgatcagcaa
      541 aacgctcagt ctttcatttg gcttcatcca gtcacttagc atgctgatca cctttaccgt
      601 tattctttgg caaagcgcgg gtatgctctc attcactgtt ggtggaacag aatggaatat
      661 ccaggggtat atggtctata ccgttgtgct cattgtgatc ggtggaactt tatttaccca
      721 taaggtgggt aaacgtattc gtccgcttaa tgtggaaaaa caacgtagcg aagcgacatt
      781 tcgaactaat cttgtgcagc ataataagca ggcagaactg attgcgctaa gcaatgctga
      841 gtctctacaa cgccaggagt tgagcgacaa ttttcatacc attaaagaga actggcatcg
      901 tttaatgaat cgtcagcggt ggcttgatta ctggcagaat atttattcgc gctcgcttag
      961 cgttcttccc tactttctgt tattgccgca gtttattagc gggcagatca atctgggcgg
     1021 actgatgaaa tcgcgccagg catttatgct ggtatcgaac aatctaagct ggtttattta
     1081 taaatatgat gaacttgctg aactggctgc ggttatcgat cgcttgtatg agttccatca
     1141 actcaccgaa cagcgcccta cgaataagcc taaaaattgc caacatgcgg tacaagtggc
     1201 tgacgcgagt attcgtactc ctgataataa gatcatatta gagaacctga actttcatgt
     1261 ttcgccaggc aaatggctat tactgaaagg ttactctggt gcgggaaaaa ccacactgct
     1321 taaaacatta tcccactgct ggccgtggtt taaaggtgat atttcttctc ctgccgacag
     1381 ttggtatgtg tcacaaacac cgttaatcaa aaccggctta ctgaaagaga ttatttgtaa
     1441 agcacttccc ctgcccgtag acgataaatc attgagcgaa gtactgcatc aggttggtct
     1501 tgggaaattg gctgcgcgta ttcatgacca cgatcgctgg ggagatattc tttccagcgg
     1561 cgaaaaacaa cgtatcgccc tggcacgatt aattttacga cgtccgaaat ggatatttct
     1621 tgacgaaact acctctcatc ttgaggaaca agaggctatc cgcttactgc gtctagtgcg
     1681 tgaaaaacta cccacaagcg gcgtcattat ggttacacat caacccggtg tctggaacct
     1741 ggccgatgat atttgtgaca ttagcgcggt tttataaaaa caataaaagc cctgctgtac
     1801 ggagatataa ataaatgaag cgagttctta ttcctggcgt cattttatgt ggcgctgatg
     1861 tggcgcaggc cgtcgatgac aaaaacatgt acatgcattt ttttgaagag atgacggtct
     1921 atgctcctgt ccctgtaccc gtaaacggca acacgcatta caccagtgaa agcatcgagc
     1981 gtttaccgac cgggaatggc aatatcagcg atctgctgag aaccaaccct gcggtacgca
     2041 tggattcaac gcaaagtacc tcgttgaacc agggagatat tcgcccggag aaaatctcta
     2101 ttcacggtgc gtcgccctat cagaatgcct atttgattga cggtattagt gccactaata
     2161 acctgaaccc agcgaatgag tccgatgcca gtagtgcaac caatattagc gggatgtcac
     2221 aggggtatta tcttgatgtc agcttactgg acaatgtgac gctttatgac agttttgtgc
     2281 cggttgaatt cggtcgcttc aatggcgggg taattgatgc aaagttcaaa cgcttcaacg
     2341 ctgatgatag cagcgtgaaa ctgggttatc gcactacgcg ttcggactgg ttaacatcgc
     2401 atatcaatga gaataacaag agcgcattta atcaaggctc ttcaggaagt acttattact
     2461 ccccagattt taaaaagaac ttttatacct tgtcgtttaa tcaggaactc gctgataact
     2521 ttggcgttac cgccggttta tcgcgccgcc agtctgatat cacccgcgcg gattatgttt
     2581 cgaatgacgg cattgtcgcc ggtcgggcac agtataaaaa cgttatcgat actgcattga
     2641 gcaaatttac ctggtttgcc agcgaccgct ttacccacga tttaacctta aaatataccg
     2701 gctccagccg tgattataat accagcacct tcccgcagtc tgatcgcgaa atgggtaata
     2761 aatcctatgg tatggcatgg gatatggata cgcagctcgc atgggccaaa ctacgtacca
     2821 ccgttggttg ggatcatatt agtgattata cccgtcacga tcatgacatc tggtacaccg
     2881 aactttcatg tacatatggt gatattacag ggcgttgcac ccgtggcgga ttaggacaca
     2941 tttcccaggc tgtagataat tacaccttca aaacacgcct ggactggcaa aaattcgccg
     3001 tgggtaatgt ttcgcatcaa ccctacttcg gcgcggaata catctattcc gatgcgtgga
     3061 ctgaacgcca taaccagtct gaatcctatg tgattaatgc tgccggaaag aaaactaacc
     3121 ataccattta ccataaaggt aaaggcagcc tgggaattga caactacaca ctgtatatgg
     3181 cggatcgcat tagctggcgt aatgtgtcat taatgcccgg cgtgcggtat gactatgaca
     3241 actatctgtc aaaccacaat atctccccgc gctttatgac ggaatgggat atttttgctg
     3301 atcaaacctc aatgattaca gcaggttata accgttacta tggcgggaat attcttgata
     3361 tgggattacg tgatatccgc aatagctgga cggaatcggt atcaggtaat aaaaccctga
     3421 cgcgttatca ggatttgaaa acgccttata acgatgaact ggcaatggga ttgcagcaaa
     3481 aaatcggtaa gaacgttatt gcgcgcgcaa actatgttta ccgtgaagcg catgatcaaa
     3541 tcagcaaaag cagtcgtacc gacagcgcga ctaaaaccac cattactgaa tataacaacg
     3601 acggcaaaac caaaacgcat tcgttcaacc tcagttttga actggccgaa cccctgcata
     3661 tcagccaggt agatattaac ccgcaaattg tctttagcta tatcaagagc aagggcaact
     3721 tgtcgttaaa caatggttat gaggagagca ataccggtga taaccaggtg gtttataacg
     3781 gtaatcttgt ctcttacgat agcgttccag tggcagattt taataaccca ttaaagatct
     3841 ccttaaacat ggatttcacg catcaaccga gcgggttggt gtgggcgaat acgctggcct
     3901 ggcaagaagc gcgtaaagct cgcattatcc tgggtaagac aaatgcgcaa tacatcagcg
     3961 aatattcaga ttacaagcag tatgttgacg aaaaactgga tagcagcctg acctgggaca
     4021 cccgcttgtc ctggacgcca caatttctga aacaacaaaa cctgacgatc agtgccgata
     4081 ttctcaatgt actggatagc aaaaccgcgg ttgatacaac gaataccggt gtggcgacct
     4141 acgccagtgg ccgtactttc tggcttgatg tcagcatgaa attttaatag ttcggatttt
     4201 acttaaccgg gtaaattcgc ccggttttcg catggagata attatgagaa acctctgttt
     4261 cttactgacg ttagtggcaa ctctgttgct ccccgggcgg ctgattgccg ccgccttacc
     4321 gcaggatgaa aagttaatta ccgggcaact ggacaatggc ttgcgatata tgatttatcc
     4381 gcatgctcat ccaaaggatc aggtaaattt atggctgcaa attcataccg gttcattgca
     4441 ggaagaagac aatgagcgcg gcgtggctca ttttgtagaa catatgatgt ttaacggcac
     4501 aaaaacatgg ccgggtaata aagtcatcga aacatttgag tcaatgggcc tgcgttttgg
     4561 tcgcgatgtt aatgcctata ccagctatga cgaaacggtg tatcaggtga gtttgccaac
     4621 tacgcagaaa caaaatctgc aacaagtgat ggcaatcttc agtgaatgga gtaatgccgc
     4681 aacctttgaa aaactcgaag tagacgctga acgcggcgta attactgagg aatggcgtgc
     4741 ccatcaggat gcgaaatggc gcacctctca ggcgcgccgc cctttcctgc tggcaaatac
     4801 ccgtaattta gaccgtgaac ctatcggcct gatggatact gtcgccacgg tcacaccggc
     4861 acaattgctc caattttatc aacgctggta tcaaccaaat aatatgacct ttatcgtggt
     4921 tggcgatatc gacagtaaag aagcgctggc gctgataaag gataatttaa gtaagcttcc
     4981 ggctaacaaa gcagctgaaa atcgcgtctg gccgacaaaa gccgaaaacc acctgcgctt
     5041 taatatcatt aatgataaag aaaaccgggt gaacggcatc gcactctatt atcgcctgcc
     5101 aatggtacaa gtgaacgatg agcaaagctt tatcgaacaa gctgaatgga gcatgttagt
     5161 tcagctgttc aatcaacgtc tgcaggaacg tatacagtcg ggcgagttga agactatttc
     5221 tggcggcact gcgcgcagcg ttaaaattgc acccgattat cagtcgctgt ttttccgtgt
     5281 aaatgcacga gacgataata tgcaggatgc tgcgaatgca ttaatggaag agttggcaac
     5341 cattgatcag catggttttt ctgctgaaga actcgatgat gtcaaatcta cccgcctcac
     5401 ctggctgaaa aatgcggttg atcagcaagc tgaacgtgat ttacgtatgc tgaccagtcg
     5461 cctggcatcc agctcattaa ataatacgcc gttcttgtcg ccggaagaga catatcaact
     5521 ttcgaaacgt ctgtggcagc aaattaccgt gcaaagtctg gcggaaaaat ggcagcagtt
     5581 aagaaagaac caggacgcat tttgggagca aatggtaaac aatgaggttg ccgccaaaaa
     5641 agcattgtct cctgcggcta tcctggcgct ggaaaaggag tacgccaaca aaaagctggc
     5701 ggcttacatc ttcccaggca gaaatttatc gttaacagta gacgctgacc cacaggcgga
     5761 aattagcagc aaagaaacgc tggctgagaa tctgacatca ttaacacttt ccaatggtgc
     5821 aagggttatt ctggcaaaat ccgcgggtga agagcaaaag ctacaaatta ttgccgtatc
     5881 gaataaaggc gatttaagtt tccctgcgca gcaaaaatca cttatcgcgc tggcaaataa
     5941 agcagttagc ggaagcggcg ttggcgaact ctcctcttcc agcctgaaac gctggagtgc
     6001 ggaaaactcg gtaaccatga gcagtaaagt cagtggcatg aatacgttgc tctccgttag
     6061 cgcgcggact aataaccctg aacctggttt tcagttgatt aaccagcgaa tcacccacag
     6121 cacgattaac gacaatattt gggcatcgct acaaaatgct caaattcagg cattgaaaac
     6181 gctcgaccag cgtccagcgg agaaattcgc ccagcagatg tatgagacgc gctatgctga
     6241 tgaccgcacg aaattactgc aagaaaatca gattgcacag tttactgccg cagatgcgct
     6301 ggctgccgat cgccagttgt tttcatctcc agcggatatc acgtttgtca ttgtcggtaa
     6361 tgtcgcagaa gacaaactcg tggcgttaat tacgcgttac ttaggatcaa tcaaacactc
     6421 tgattcgcca ttagccgcag gtaaaccatt aactcgcgcg acggacaacg catcggttac
     6481 tgtaaaggaa caaaatgaac ctgtggcaca ggtttcacag tggaagcgtt atgattcccg
     6541 gacacctgtt aatctggcga cgcgtatggc gctcgatgct tttaacgtcg cactggcaaa
     6601 agatctacgc gttaatattc gtgaacaggc atctggggca tacagcgttt cttctcgcct
     6661 ctcggttgat cctcaggcta aagatatcag tcatttgctg gcttttactt gtcaaccaga
     6721 acgacatgat gaactgttaa cgttagcgaa tgaagtgatg gttaagcgtt tgactaaagg
     6781 gatcagtgag caagaactga acgaatacca gcaaaacgtt cagcgcagcc tcgatatcca
     6841 acagcgtagc gttcaacaat tagcgaacac tattgtaaat agtcttattc aatatgacga
     6901 tcctgcagca tggactgagc aggagcaatt gttgaaacaa atgacggtag agaatgttaa
     6961 cactgccgtt aaacaatatc tttctcatcc ggtaaatact tataccggag tattattgcc
     7021 aaaataataa cagccccgtc aacacatcgt tggcggggat tttagcaata ttcgctattt
     7081 ttatgtaata attttataaa tgcgttcaaa ataataaccg cgtactaata gtgatatttt
     7141 aaggtctgat ttttacgtga taattcagga ggcacagaat acgcataaaa ataacagcat
     7201 aaaacacctt actaccaccc aagagctccc tactatattg tttttcaatg aaaaaatatt
     7261 attcacgcaa tatctcacca taaataacat taggattttg ttatttaaac acgagtcctt
     7321 tgcacttgct tactttatcg ataaatccta cttttttaat gcgatccaat cattttaagg
     7381 agtttaaaat ggataagaag caagtaacgg atttaaggtc ggaactactc gattcacgtt
     7441 ttggtgcgaa gtctatttcc actatcgcag aatcaaaacg ttttccgctg cacgaaatgc
     7501 gcgacgatgt cgcatttcag attatcaatg acgaattata tcttgatggc aacgctcgtc
     7561 agaacctggc cactttctgc cagacctggg acgacgaaaa cgtccacaag ttgatggatt
     7621 tatccattaa caaaaactgg atcgacaaag aagaatatcc gcaatctgca gccatcgacc
     7681 tgcgttgcgt aaatatggtt gccgatctgt ggcatgcgcc tgcgccgaaa aatggtcagg
     7741 ccgttggcac caacaccatt ggttcttccg aggcctgtat gctcggcggg atggcgatga
     7801 aatggcgttg gcgcaagcgt atggaagctg caggcaaacc aacggataaa ccaaacctgg
     7861 tgtgcggccc ggtgcaaatc tgctggcata aattcgcccg ctactgggat gtggagctgc
     7921 gtgagatccc tatgcgcccc ggtcagttgt ttatggatcc gaaacgcatg attgaagcct
     7981 gcgacgaaaa taccatcggc gtggtgccga ctttcggcgt gacctacacc ggtaactatg
     8041 agttcccgca accgctgcac gatgcgctgg ataaattcca ggccgacacc ggtatcgaca
     8101 tcgacatgca catcgacgcc gccagtggtg gcttcctggc accgttcgtc gccccggata
     8161 tcgtctggga cttccgcctg ccgcgtgtga aatcgatcag tgcttcaggc cacaaattcg
     8221 gtctggctcc gctgggctgc ggctgggtta tctggcgtga tgaagaagcg ctgccgcagg
     8281 aactggtgtt caacgttgac tacctcggtg gtcagattgg tactttcgcc atcaacttct
     8341 cccgcccggc gggtcaggta attgcacagt actatgaatt cctgcgcctc ggtcgtgaag
     8401 gctataccaa agtacagaac gcctcttacc aggttgccgc ttatctggcg gatgaaatcg
     8461 ccaaactggg gccgtatgag ttcatctgta ccggtcgccc ggacgaaggc attccggcgg
     8521 tttgcttcaa actgaaagat ggtgaagatc cgggatacac cctgtatgac ctctctgaac
     8581 gtctgcgtct gcgcggctgg caggttccgg ccttcactct cggcggtgaa gccaccgaca
     8641 tcgtggtgat gcgcattatg tgtcgtcgcg gcttcgaaat ggactttgct gaactgttgc
     8701 tggaagacta caaagcctcc ctgaaatatc tcagtgatca cccgaaactg cagggtattg
     8761 cccaacagaa cagctttaaa catacctgat aacgtttaac ggtaacggtg tcccgaaacg
     8821 aacccgtttc gggacaattt ccaaagtctg ttcactggca ttagcaacgg aaaatattgt
     8881 tctgaatacg cttcagaaca aaacaggtgc ggttccgaca ggaataccgt tttaggggga
     8941 taatatggct acattagtac agacaggtaa agctaagcag ctcacattac tcggcttttt
     9001 tgccataacg gcatcgatgg taatggctgt ttatgaatac cctaccttcg caacatcggg
     9061 cttttcatta gtcttcttcc tgctattagg cgggatttta tggtttattc ccgtgggact
     9121 ttgtgctgcg gaaatggcca ccgtcgacgg ctgggaagaa ggtggtgtct tcgcctgggt
     9181 atcaaatact ctggggccga gatggggatt tgcagcgatc tcatttggct atctgcaaat
     9241 cgccattggt tttattccga tgctctattt cgtgttaggg gcactctcct acatcctgaa
     9301 atggccagcg ctgaatgaag accccattac caaaactatt gcagcactca tcattctttg
     9361 ggcgctggca ttaacgcagt ttggtggcac gaaatacacg gcgcgaattg ctaaagttgg
     9421 cttcttcgcc ggtatcctgt tacctgcatt tattttgatt gcattagcgg ctatttatct
     9481 gcactccggt gcccccgttg ctatcgaaat ggattcgaag accttcttcc ctgacttctc
     9541 taaagtgggc accctggttg tatttgttgc cttcattttg agttatatgg gcgtagaagc
     9601 ttccgcaacc cacgtcaatg aaatgagtaa cccagggcgc gactatccac tggctatgtt
     9661 actgctgatg gtggcggcaa tctgcttaag ctctgttggc ggtttgtcta ttgcgatggt
     9721 cattccgggt aatgaaatca acctctccgc aggggtaatg caaaccttta ccgttctgat
     9781 gtcccatgtg gcaccggaaa ttgagtggac ggttcgcgtg atctccgcac tgctgttgct
     9841 gggtgttctg gcggaaatcg cctcctggat tgttggtcct tctcgcggga tgtatgtcac
     9901 agcgcagaaa aacctgctgc cagcggcatt cgctaaaatg aacaaaaatg gcgtaccggt
     9961 aacgctggtc atttcgcagc tggtgattac ttctatcgcg ttgatcatcc tcaccaatac
    10021 cggtggcggt aacaacatgt ccttcctgat cgcactggcg ctgacggtgg tgatttatct
    10081 gtgtgcttat ttcatgctgt ttattggcta cattgtgttg gttcttaaac atcctgactt
    10141 aaaacgcaca tttaatatcc ctggtggtaa aggggtgaaa ctggtcgtgg caattgtcgg
    10201 tctgctgact tcaattatgg cgtttattgt ttccttcctg ccgccggata acatccaggg
    10261 tgactctacc gatatgtatg ttgaattact ggtcgttagt ttcctggtgg tacttgccct
    10321 gccctttatt ctctatgctg ttcatgatcg taaaggcaaa gcgaataccg gcgtcactct
    10381 ggagccaatc aacagtcaga acgcaccaaa aggtcacttc ttcctgcacc cgcgtgcacg
    10441 ttcaccacac tatattgtga tgaatgacaa gaaacactaa ctatgtctga atggttataa
    10501 gataagggag cgataattca tcgctccctt tttcgtgctt gctgtcgtat tgacttaacc
    10561 gggataagta cgagaatgag cgcacatctg tttaccggaa actagcacat atggatatct
    10621 gctcccgaaa caagaaatta acgattagaa gaccagcgat actggttgca ctggcacttt
    10681 tactgtgtag ttgtaaaagc acgcctccag agtccatggt gacaccacca gcgggttcaa
    10741 agccaccagc cacgacgcaa caatcgtcac aaccgatgcg tggcatctgg ctggccacgg
    10801 tgtctcgcct cgactggcca ccggtttcct cggttaacat tagtaacccc accagccggg
    10861 cccgtgtaca acaacaggcg atgatcgaca aactggatca tttgcaacgt ctcggtataa
    10921 acacggtctt tttccaggtc aagccggacg gtaccgccct gtggccatcg aaaattttgc
    10981 cgtggtccga tcttatgacc ggtaagattg gtgaaaatcc gggttacgat ccgctgcaat
    11041 ttatgctcga cgaagcccac aagcgtggga tgaaagtaca cgcctggttt aacccctatc
    11101 gcgtatcggt taatacgaag cccggtacta tcagggaact gaatagcact ctgtctcaac
    11161 aaccggcgag cgtctatgtg caacaccgtg actggatcag aacgtctggc gatcgctttg
    11221 tcctcgaccc gggcatccct gaggttcagg actggatcac atcaatagtc gcagaagtgg
    11281 tttcccgcta tccggtagat ggcgtgcagt ttgacgacta tttctatact gagtcaccgg
    11341 gttcacggct aaatgataac gaaacgtacc gtaaatacgg aggcgcattt gcgtcaaaag
    11401 cagactggcg gcgcaacaat actcagcagt taattgcaaa ggtatcgcac accattaaaa
    11461 gcattaagcc gggagtcgaa tttggtgtta gcccggcagg cgtgtggcgt aaccgatcac
    11521 acgatccgct cggttccgat acccgaggcg cggcagccta tgacgaatcc tacgctgaca
    11581 ctcgtcgatg ggtggaacaa ggattgctgg attacattgc tccccaaatt tactggccgt
    11641 tctcacggag tgccgcgcgt tatgacgtgt tggcaaaatg gtgggcggat gtcgttaaac
    11701 cgaccagaac ccgcctgtat atcggtatcg ccttctataa agtgggtgaa ccttcaaaga
    11761 tagagccaga ctggatgatt aacggcggcg taccggagct gaaaaagcag ctcgatctta
    11821 acgatgcggt accagaaatt agcggcacca tcttgttccg tgaggactat ctgaataaac
    11881 cgcagactca acaagcggtc agctatctgc aaagtcgttg gggcagttaa actcccattc
    11941 ttactttgta acaagccggt gctttaccca ctggcttgtt accctcttga actatcccac
    12001 cagcaatcat gcttactaaa gtcaaattaa ccacgcaatt aaccacgctt agttaaacag
    12061 ccataaatcc cctaataaaa atgtatgaat atgaaaaacc atacgatatt cgcagtcacc
    12121 gaaaaataat tacgatgggc tatactcttc tgaagaacag tagcgtagat tgaaactgtc
    12181 ctaagtcgct tatttctttt cagtatatct tcatatttca ggagaatatg gtgcagttag
    12241 tcgatctcgc acgtcgcgtt tcattttgat tccgtctgat ttcccagcct tataagggtg
    12301 tgaattatgg agatgtattt taaaagaatg aaagatgagt ggaccggact tgtcgagcag
    12361 gcagatccgc ccattcgtgc taaagccgcg gaaattgccg ttgcgcatgc tcattatctg
    12421 agtatcgagt tttatcgaat tgtccgcatc gacccgcatg ccgaagaatt cttgagtaat
    12481 gaacaagttg agcggcagtt gaaaagtgcg atggaacgct ggattattaa cgtgctttct
    12541 gcccaggttg acgatgtcga aaggctaata caaatccagc ataccgtcgc ggaagtgcat
    12601 gcccgcatag gaattccggt agaaattgtc gagatggggt ttcgggtgct gaaaaagatc
    12661 ctctatccgg taatcttctc ttcggattat tctgccgcag aaaaacttca ggtctaccat
    12721 ttctcgatta acagtattga tatcgctatg gaagtgatga cccgcgcgtt tacctttagt
    12781 gacagtagtg cctcaaaaga agatgaaaac tatcgtatct tctcgttact ggaaaacgcc
    12841 gaagaagaaa aagaacggca aatagcctca atactttcat gggaaataga tattatctat
    12901 aaaatcctgc tggattctga tttaggcagt agtttgcctt taagccaggc tgattttggc
    12961 ctgtggttta accataaagg tcgacattat ttcagcggca ttgctgaagt gggccatatc
    13021 tctcgtctga ttcaggattt cgacggtatt ttcaatcaaa ccatgcgtaa caccaggaat
    13081 ttgaataaca gaagtctgcg ggtgaaattt ttattacaga taagaaatac cgtatcgcaa
    13141 attattacct tgctgcgtga attgtttgaa gaagtatcgc gccacgaagt cggtatggat
    13201 gtactgacga aattacttaa ccgccgtttc ctgccgacta tcttcaaacg cgaaattgcc
    13261 catgccaacc ggaccggtac accgctgtca gtgctgatta ttgacgttga taaattcaaa
    13321 gagatcaacg atacgtgggg ccataacact ggcgatgaaa ttctgcgtaa agtctctcag
    13381 gccttttatg acaacgtccg cagtagtgat tatgttttcc gctacggggg cgatgaattt
    13441 atcattgttt tgactgaagc ttctgaaaac gaaacgttac gtaccgcaga acgtattcgc
    13501 agtcgggtgg agaaaaccaa actgaaagcc gcaaacggcg aagatattgc cctctcactt
    13561 tccatcggtg ccgccatgtt taatggtcat cctgactatg agcgcctcat tcaaatagcc
    13621 gatgaagctc tgtatatcgc caaaagacga ggtagaaacc gtgttgaact ctggaaagcc
    13681 agtctttaga tgcgccagga tgcagaggta atcatgaagc taaccgatgc ggataatgcc
    13741 gccgatggca tttttttccc cgcccttgag caaaatatga tgggtgcggt gttaattaac
    13801 gaaaatgatg aagtgatgtt tttcaacccc gccgcagaga agctctgggg gtacaaacgt
    13861 gaagaagtca ttggcaataa cattgatatg ctgattccgc gggatttgcg tcctgcgcat
    13921 cctgaataca ttcgtcacaa ccgtgaaggc ggtaaagcgc gcgtcgaggg gatgagtcgg
    13981 gagctgcagc tggagaaaaa agacggcagt aaaatctgga cccgttttgc gctatcgaaa
    14041 gtgagcgccg aggggaaagt ttattacctg gcgctggtac gggatgccag cgtagaaatg
    14101 gcgcaaaaag aacagaccag gcaattgatt attgccgttg accatctcga ccgaccggtg
    14161 attgtcctcg atccggaacg ccatattgtg cagtgcaatc gcgcatttac cgaaatgttt
    14221 ggttactgca ttagcgaagc cagcggtatg cagcctgata cactcctgaa cattcctgaa
    14281 ttccctgccg ataaccgcat tcgtttacaa cagttgcttt ggaaaaccgc ccgcgatcag
    14341 gacgaatttc tgctgttgac gcgcaccggt gaaaaaatct ggattaaagc ttctatcagc
    14401 ccggtttatg acgtgctcgc gcatctgcag aacctggtaa tgaccttctc ggatatcacc
    14461 gaagaacggc aaatccgcca gcttgaaggc aatattctcg ccgccatgtg cagcagcccg
    14521 ccatttcatg aaatggggga aatcatttgt cgtaacatcg aatctgttct caacgaatcg
    14581 catgtttcgc tgttcgcact gcgcaacggg atgccgatac actgggcgtc atcttcgcac
    14641 ggtgcagaag ttcaaaatgc gcaaagctgg tcagcgacca ttcgtcagcg tgatggcgcg
    14701 cctgcgggga tcctgcaaat taaaacctcg tcaggagcag aaaccagcgc ctttatcgaa
    14761 cgcgtggcag atatcagcca gcatatggcc gcgctggcgc tggaacagga aaaaagccgt
    14821 cagcatattg aacaactcat ccaatttgat ccgatgaccg gtctgccaaa tcgcaataac
    14881 ctgcacaatt acctcgatga cctggtcgac aaagccgtct ctccagtatc ggtgttgacc
    14941 atattcagga tgtgattgat agccttggct atgcgtgggc cgatcaggca ttgctggaag
    15001 tggtcaatcg ctttcgtgaa aaactcaaac cggatcagta tctctgtcgt atcgaaggta
    15061 cgcagtttgt cctcgtgagc ctggaaaacg acgtcagtaa cattacccag atcgccgatg
    15121 agctacggaa tgtggtcagc aagccgataa tgattgacga taaccccttc ccgcttacct
    15181 tgagtattgg catcagctac gacgtgggta aaaatcgcga ttacctgctc tccactgccc
    15241 ataacgcgat ggattatatt cgcaagaatg gcggtaacgg ctggcagttc ttcagcccgg
    15301 cgatgaacga aatggtaaaa gagcgtttgg ttttaggcgc agcgctgaaa gaagcgatta
    15361 gcaataacca actgaaactg gtttaccagc cgcaaatctt cgcagaaacg ggtgaactct
    15421 acggcatcga agcccttgct cgctggcacg atccccagca tggtcatgtg cccccttcac
    15481 ggtttattcc tctcgcagaa gagattggtg aaatcgaaaa tattgggcgc tgggtcatcg
    15541 cggaagcttg ccgtcagtta gcagaatggc gtagccagaa tattcatatc ccggcgttat
    15601 ccgtgaactt gtcggcgctg cactttcgca gtaatcagct gcctaatcag gtgtctgatg
    15661 caatgcaagc ctggggtatt gacggccacc agctgacggt agaaatcacg gaaagcatga
    15721 tgatggaaca cgataccgaa atctttaagc gcattcagat cctgcgtgat atgggcgtgg
    15781 gtttatcggt agatgatttt ggcacgggct tttccggatt atcccgctta gtcagtcttc
    15841 cggtaacgga aatcaaaatt gacaaaagtt ttgtcgatcg ttgtctgacg gaaaaacgca
    15901 tcctggcctt acttgaagcc attaccagca ttgggcaaag cctcaattta accgtcgtgg
    15961 cggaaggcgt cgaaaccaaa gagcaatttg agatgctacg aaagatccac tgtcgcgtta
    16021 ttcagggata tttcttttcc cgccccctac ccgccgaaga aattccaggc tggatgagca
    16081 gcgtgttacc gctgaaaatc tgacaaattc ctctctcccg cactcgcggg ttttcattta
    16141 acgtgacact gtcacttaaa tactgtgatt tcagctacga ttccgggaga ttccttcctt
    16201 aacttcccct cttgcaccaa aactgcacta cgctgcacaa atgttgatca aaagttaaaa
    16261 aatataactt taagccagca ttctggcgct tatcccggca tggcatgaga tctgcataag
    16321 cggaaagcgc agcaattttt gtcttataca ggaaccgttt atgtcggata ccaccgaact
    16381 ggttgattta gccgtgatct tccctgatct ggagatcgaa ttgaaatacg cctgcgctga
    16441 taacatcaca ggtaaagcta tttaccagca agcgcgttgt ctgttacaca aggatgcgat
    16501 taccgcgctg gcgaaaagta tcagtatcgc ccagctatca ggcttacaac tggtgattta
    16561 cgatgcgtat cgcccacaac aagcacaggc gatgttgtgg caagcctgcc cagacccgca
    16621 atatgttgtt gatgtgacgg tcggttctaa tcacagccgt ggcacggcga tcgacctgac
    16681 gcttcgtgat gagcacggga acatcctgga tatgggcgca gggttcgatg aaatgcacga
    16741 gcgctcccat gcctatcacc cttccgtccc gcccgccgct cagcgcaatc gcctgttgct
    16801 gaatgcgata atgactggtg gcggttttgt cggtatctcc agcgaatggt ggcacttcga
    16861 attaccccag gcagcgagtt accctctgct cgctgatcaa ttcacctgtt ttatatcccc
    16921 tggcacacag cacgtcagtt aatcaggaaa ctgccatgaa gagatcgata tcgtttcgtc
    16981 ccacattgct cgcgctcgtc cttgccacaa ctttcccggt tgcgcacgcc gccgtaccga
    17041 aagatatgct ggtgattggt aaggccgccg atccacaaac cctcgacccg gcggtaacaa
    17101 tagataataa cgactggaca gtgacctacc cgtcttatca gcgactggtt cagtacaaaa
    17161 cggacggtga taaaggctca accgacgttg aaggcgatct ggcaagtagc tggaaagcgt
    17221 ctaacgatca aaaagagtgg acgttcaccc tgaaagataa tgctaaattt gccgatggca
    17281 cacctgtcac tgccgaagca gtaaaactct cttttgagcg gttactaaaa atcggccagg
    17341 ggccagcaga agcatttccc aaagatttaa agattgatgc tcccgacgaa catacggtga
    17401 agtttaccct tagccagcca tttgcaccgt tcctctacac gctggcgaat gacggtgcat
    17461 ccattatcaa tccggcggta ttaaaggaac atgcggcgga tgatgcccgc ggcttcctcg
    17521 cgcaaaatac cgccggttcc ggaccattta tgctgaaaag ctggcaaaaa ggtcagcaat
    17581 tagttctggt gccaaatccg cattaccccg gcaataaacc gaactttaag cgagtatcgg
    17641 taaaaattat cggtgaaagt gcctcccgtc gcctgcagct ctcccgtggc gacattgaca
    17701 ttgccgatgc gctgccggtg gatcaactca acgccctgaa gcaggaaaac aaagtcaatg
    17761 tggcagagta tccgtcactg cgcgtcacct atctgtatct gaataacagc aaagcgccac
    17821 ttaatcaggc ggatctgcgg cgggccattt cctggtctac cgattatcag ggcatggtta
    17881 acggcattct gagtggtaac ggaaaacaaa tgcgcggccc gattccggaa ggcatgtggg
    17941 gctacgatgc gacggcaatg caatacaacc atgacgaaac gaaagccaaa gccgaatggg
    18001 ataaagtgac gagcaaaccc accagcctga cgtttctcta ctccgataac gatccgaact
    18061 gggaaccgat cgctctggca acacaatcca gcctcaacaa gctgggcatc aatgtgaagc
    18121 tggaaaagct ggcgaacgcc accatgcgcg acagagtggg taaaggtgat tacgacattg
    18181 cgattggcaa ctggagtccg gattttgccg acccgtatat gtttatgaat tactggtttg
    18241 agtcagacaa aaaaggtctg ccgggtaacc gctcgttcta tgaaaacagt gaggtcgata
    18301 agttactgcg caatgcgctt gcgaccaccg accagacgca gcgtacccga gactaccagc
    18361 aggcacagaa aatcgtcatt gatgacgctg cttatgtgta cctgttccag aaaaactacc
    18421 aactggcgat gaacaaagag gtgaaaggct ttgtgttcaa tcccatgctg gaacaggtct
    18481 tcaatatcaa taccatgagt aaataacgtg accttctgga gtattttacg ccaacgctgc
    18541 tgggggctgg tgctcgtggt ggcgggcgtc tgcgtgatta cgtttattat ctcgcatctg
    18601 atccccggcg atccggcgcg gttactggcg ggtgaccgcg ccagcgatgc tatcgtggaa
    18661 aatattcgcc agcaactggg actggaccag ccactgtatg tacagtttta ccgctacgtc
    18721 agcgacctgt ttcatggtga cctgggaaca tccattcgta ctgggcgtcc ggtgctggaa
    18781 gagttgcgta tatttttccc ggcgacgctg gagctggctt tttgtgccct gctgctggca
    18841 ctcttgattg gcatcccgcg gggcatactc tctgcagtct ggcgaaatcg ctggctggat
    18901 catctggtgc gaataatggc gattaccgga atctccacac ctgcgttctg gcttgggctg
    18961 ggcgtcatcg tgctgtttta tggtcatctg caaatacttc ccggcggcgg aaggcttgat
    19021 gactggctgg atccaccaac gcacgttacc ggcttttatc tgctcgatgc gttgcttgaa
    19081 ggcaacggtg aagtcttctt caatgcgttg caacatctca tcttaccggc attaacgctg
    19141 gcgtttgttc acctggggat tgtcgcccgc cagatccgct cagcgatgct ggaacaattg
    19201 agtgaagact acattcgtac cgcccgggcc agcggcttgc ccggctggta tatcgttcta
    19261 tgttatgcgc tacccaatgc gttgatccct tcgattaccg tattggggct ggcgctgggc
    19321 gatttgttgt atggcgcagt gctcaccgaa accgtttttg cctggcccgg aatgggggca
    19381 tgggtagtga catcaataca ggcgctcgac ttcccggcag tgatgggctt tgccgtcgtg
    19441 gtttcatttg cttatgtgct ggtcaacctg gtggtggatt tgctctattt gtggattgat
    19501 ccgcgtatcg gacgtggagg tggtgaatga tgctaagcga ggaaacgtcc gccgtacgcc
    19561 cacaaaaaca aacgcgattt aacggtgcaa aactggtatg gatgctgaaa ggcagtccgc
    19621 tcaccgtgac cggcgcagtc atcattgtat taatgctact gatgatgatt ttttcaccgt
    19681 ggctggcgac gcatgatccc aacgccattg atttaaccgc ccgccttttg ccgccttctg
    19741 cggcgcactg gtttggcacc gatgaagtgg gacgcgatct gttcagccgc gtactggtcg
    19801 gcagtcagca atcaattctc gccggattag tcgtggttgc cattgcgggt atgattggtt
    19861 cgctactcgg atgtctatcc ggtgtgcttg gcggacgcgc agacgccatc atcatgcgca
    19921 tcatggacat tatgctgtcg attccttcgt tggtactgac aatggcactg gctgccgctc
    19981 tcgggccgag tttgtttaac gccatgctgg ctattgctat tgtgcgaatt cccttttatg
    20041 tgcgcctggc gcgggggcaa gcattagttg tacgccagta tacctatgtt caggcggcga
    20101 aaacctttgg cgcgtctcgt tggcatctga tcaactggca tattttacgt aactccctac
    20161 cgccgctgat cgtgcaggca tcgctggata tcggtagcgc gattttaatg gccgccacgt
    20221 tgggatttat tggcctgggt gctcaacaac cgagtgctga atggggcgcg atggtagcaa
    20281 atggtcgcaa ctatgtactc gatcagtggt ggtattgcgc atttccgggg gcagcgattt
    20341 tgcttaccgc cgtcgggttt aatctctttg gtgatggtat tcgcgatctg cttgacccga
    20401 aagcaggagg aaagcagtca tgacccaacc cgttctggac attcaacaac tgcatttgag
    20461 tttccccggt tttaacggcg acgttcacgc gctcaacaat gtgtccttgc agattaaccg
    20521 cggtgaaatt gtcggtctgg tgggagaatc cggctcaggt aaatcagtca ccgcaatgct
    20581 gattatgcgt ctgctaccga cgggcagtta ttgcgtacat cgggggcaga tttcactgct
    20641 gggagacgat gttcttaacg cgcgggaaaa acagcttcgt cagtggcgcg gcgcacgagt
    20701 ggcgatgatc tttcaggaac cgatgaccgc cctcaatccg acacgtcgaa taggtcttca
    20761 gatgatggac gtgatccgcc atcatcaacc aataagtcgt cgggaagcca gagctaaagc
    20821 gattgccctg ctggaagaga tgcaaatccc ggataccgtg gaagttatgt cgcgctatcc
    20881 gtttgagctt tcaggtggta tgcgccagcg ggtaatgatt gcgctggcat tctcctgcga
    20941 accgcaattg attattgccg acgaaccgac tacggcgctg gacgtcacgg tacagttgca
    21001 ggtactgcgt ttgcttaaac ataaagctcg cgacagtgga actgcggtac tgttcatcag
    21061 ccatgatatg gccgtggtgt cgcaactgtg cgatagcgtt tacgtgatgt atgccggaag
    21121 cgtgattgag agcggcgtga cggcagacgt tatccatcat ccccggcatc cgtataccat
    21181 tggtttgctg caatgcgcac cagaacatgg agtaccacgc cagccattac ccgccattcc
    21241 agggacggta ccaaatctca cccatttgcc tgacggctgc gcttttcgcg atcgttgcta
    21301 tgcggcaggt gcacagtgtg aaaacgtccc ggcgctgaca gcgtgtggtg acaacaacca
    21361 gcgctgcgcc tgttggtatc cgcagcagga ggtcataagt gtctgacacg ttattaacgt
    21421 tacgcgacgt ccatatcaat ttcccggcgc gtaaaaactg gcttggtaaa actacggaac
    21481 atgttcatgc cattaatggt attgatttac agatccgccg tggtgaaacc ttagggatcg
    21541 tcggcgagtc aggctgcggc aaaagcaccc tcgcacagct tttaatgggt atgctgcaac
    21601 cgagccacgg gcagtacatc cgttctggct cacaacgcat tatgcagatg atgtttcagg
    21661 acccgctctc ttcgcttaat ccgcgcttac cggtgtggcg catcatcaca gaaccgctct
    21721 ggatagctaa gcatagtagt gaacaacagc ggcgagcgtt ggcagaggag ctggctgtgc
    21781 aggtgggtat tcgtccggag tatctcgacc gcctgcctca tgcgttctcc ggcgggcagc
    21841 ggcaacgcat cgccattgcc agagcactct cttcgcagcc tgacgtgatt gtgcttgatg
    21901 agccaacctc tgcgctggat atctccgtgc aggcgcagat cctcaattta ctggtaacgc
    21961 tacaggaaaa tcacgggctg acttatgtgc tgatttcaca caatgtctcg gtgatacgtc
    22021 atatgagcga tcgggtggcg gtgatgtatc tcgggcagat tgtggaatta ggggaagcgc
    22081 aacaggtgct aacggctccg gcacatccat acacccgatt attgctggat tccctccccg
    22141 ccattgataa accactggag gaagaatggg cattacgtaa aacggatctg ccaggaaacc
    22201 gcacgttgcc acagggctgt tttttccgcg aacgttgccc ccttgcaacc agcggatgcg
    22261 aagtccggca atcattaacg acgcgggctg acggacgtga gatccgttgc tggcgggcgc
    22321 tgtagagatg ctaacaacgc atcaggcatt catgcgcctg acgcgtcatc cggcaatgct
    22381 ttacgatttc aactggtaat ccagcgtaat ttccgctttc agtacctgag agaccgggca
    22441 tcctgctttt gctttctgga tgatgccgtc aaaggtagag gcatcaatac ccggcaccgc
    22501 aacttcactc ttcagtgcga ttttcgtaat cgcaaaaccg gcatccactt tatccagcga
    22561 cacatcggcg gtggtatcaa tcgatgttgg cgtgaatccc gcttccccca gcattaatga
    22621 aagcgccatt gagaaacatg cggcatgcgc tgcgccaatc agttcttcag ggttggttcc
    22681 tttttcgcct tcaaaacgcg tgttaaatcc atacggctgt tggttcagca cgccactctc
    22741 ggtggatact gttcccttcc cgcgtttgat atcgctttcc cagtgtgcct gacctttctt
    22801 atggattgtc attgttgctc tcctgtgggc ttaaaaagga aattatagac tacgtgagaa
    22861 accgcacgag gataagcagg atattccgaa taaaatacgg caaaaatatt acttctgcac
    22921 ttaaattaaa ttcccacaat agataaccgg aaatatatat ctcaagagag attgcatcaa
    22981 tattaatcaa ctcatcacaa taataatacc tatcaatatc atggggttaa aatataaaaa
    23041 tcatcaacca ggactaatct taacaacgaa gcggcaaata tttgccatgc tgaatgtgct
    23101 ttataaatct gcgatccgta gcagacacta taaatacaca gacacggaga atcactatgt
    23161 ttacttatta tcaggcagaa aattcaacag cagaacccgc tctggttaat gccattgagc
    23221 aaggccttcg ggcagagcac ggtgttgtca ctgaagatga cattctcatg gagctgacca
    23281 aatgggtgga agcttctgat aacgacatcc tcagtgatat ctaccagcaa acgatcaatt
    23341 atgtggtcag tggccagcac cctacgcttt aagatgctat gcttgatcgg caacctaatt
    23401 taggggttta gcacgtgttt cttcgctacg gcgatgttgt ccttaaaact agcaacagga
    23461 ttgaggagtt aaaatgaaat cgaaccgtca ggcacgtcat attcttggac tggaccataa
    23521 aatatctaac cagcgcaaaa tagttaccga aggtgacaaa tccagcgtgg taaataaccc
    23581 aaccggcaga aaacgccccg ctgaaaagta attcataacc atcagtcctc aatgacgatt
    23641 aaacaccatt gcctgcgcaa tggtgttttt gtttttatct gctttatact tggggccgac
    23701 gccctggcgg taaagcaaag acgataaaag ccccccaggg atggatattc aaaaaagagt
    23761 gagtgacatg gaaccaaaaa caaaaaaaca gcgttcgctt tatatccctt acgctggccc
    23821 tgtactgctg gaatttccgt tgttgaataa aggcagtgcc ttcagcatgg aagaacgccg
    23881 taacttcaac ctgctggggt tactgccgga agtggtcgaa accatcgaag aacaagcgga
    23941 acgagcatgg atccagtatc agggattcaa aaccgaaatc gacaaacaca tctacctgcg
    24001 taacatccag gacaccaacg aaaccctctt ctaccgtctg gtaaacaatc atcttgatga
    24061 gatgatgcct gttatttata ccccaaccgt cggcgcagcc tgtgagcgtt tttctgagat
    24121 ctaccgccgt tcacgcggcg tgtttatctc ttaccagaac cgccacaata tggacgatat
    24181 tctgcaaaac gtgccgaacc ataatattaa agtgattgtg gtgactgacg gtgaacgtat
    24241 tctggggctt ggtgaccagg gcatcggcgg gatgggcatt ccgatcggta aactgtcgct
    24301 ctataccgcc tgtggcggca tcagcccggc gtataccctt ccggtggtgc tggatgtcgg
    24361 aacgaacaac caacagctgc ttaacgatcc gctgtatatg ggctggcgta atccgcgtat
    24421 cactgacgac gaatactatg aattcgttga tgaatttatc caggctgtga aacaacgctg
    24481 gccagacgtg ctgttgcagt ttgaagactt tgctcaaaaa aatgcgatgc cgttacttaa
    24541 ccgctatcgc aatgaaattt gttcctttaa cgatgacatt cagggcaccg cggcggtaac
    24601 agtcggcacc ctgatcgcag caagccgcgc ggcaggtggt cagttaagcg agaaaaaaat
    24661 cgtcttcctt ggcgcaggtt cagcgggatg cggcattgcc gaaatgatca tcgcccagac
    24721 ccagcgcgaa ggattaagcg aggaagcggc gcggcagaaa gtctttatgg tcgatcgctt
    24781 tggcctgctg accgacaaga tgccgaacct gctgcctttc cagaccaaac tggtgcagaa
    24841 gcgcgaaaac ctcagtgaca gggataccga cagcgatgtg ctgtcactgc tggatgtggt
    24901 gcgcaatgta aaaccagata ttctgatcgg cgtctcagga cagaccgggc tgtttacgga
    24961 agagatcatc cgtgagatgc ataaacactg tccgcgtccg atcgtgatgc cgctgtccaa
    25021 cccgacgtca cgcgtggaag ccacaccgca ggacattatc gcctggactg aaggtaacgc
    25081 gctggtcgcc acgggcagtc cgtttaatcc agtggtatgg aaagataaaa tctaccctat
    25141 cgcccagtgt aacaacgcct ttattttccc gggcatcggg ctgggtgtta ttgcttccgg
    25201 cgcgtcacgt atcaccgatg agatgctgat gtcggcaagt gaaacgcttg cccagtattc
    25261 gccgctggtc ctgaacggcg aaggtctggt actaccggaa ctaaaagata ttcagaaagt
    25321 ctcccgcgca attgcgtttg cggttggcaa aatggcgcag cagcaaggcg tggcggtgaa
    25381 aacgtctgcc gaagctttgc aacaagccat tgacgataat ttctggcaag ccgaataccg
    25441 cgactaccgc cgtacctcca tctaagcctg cgcccggtag tgaaggctac cgggctattt
    25501 ccctctccct ttttcagatc tcatccatac tgggtagtgg cgaataaatc tcatttgcct
    25561 cacctgctat gcagaacatc atccgaaaag gaggaactat gaaggctgca gttgttacga
    25621 aggatcatca tgttgacgtt acggataaaa cactgcgctc actgaaacat ggcgaagccc
    25681 tgctgaaaat ggagtgttgt ggtgtatgtc ataccgatct tcatgttaag aatggcgatt
    25741 ttggtgacaa aaccggcgta attctgggcc atgaagggat tggtgtggtg gcagaagtgg
    25801 gtccaggtgt cacctcatta aaaccaggcg atcgtgccag cgtggcgtgg ttctacgaag
    25861 gatgcggtca ttgcgaatac tgtaacagtg gtaacgaaac gctctgccgt tcagttaaaa
    25921 atgccggata cagcgttgat ggcgggatgg cggaagagtg catcgtggtc gccgattacg
    25981 cggtaaaagt gccagatggt ctggactcgg cggcggccag cagcattacc tgtgcggggg
    26041 tcaccaccta caaagccgtt aagctgtcaa aaattcgtcc cgggcagtgg attgctatct
    26101 acggtcttgg cggtctgggt aacctcgccc tgcaatacgc gaagaatgtc tttaacgcga
    26161 aagtgatcgc cattgatgtc aatgatgagc agttaaaact ggcaaccgaa atgggtgcag
    26221 atttagcgat taactcacgc accgaagacg ccgccaaaat tgtgcaggag aaaaccggtg
    26281 gcgctcacgc tgcggtggta acagcagtag ctaaagctgc gtttaactcg gcagttgatg
    26341 ctgtccgtgc aggcggtcgt gttgtggctg tcggtctgcc gccggagtct atgagcctgg
    26401 atatcccacg tcttgtgctg gatggcattg aggtggtcgg ttcgctggtc ggcacgcgcc
    26461 aggatctaac tgaagccttc cagtttgccg ccgaaggtaa agtggtgccg aaagtcgccc
    26521 tgcgtccgtt agcggacatc aacaccatct ttaccgagat ggaagaaggc aaaatccgtg
    26581 gccgtatggt gattgatttc cgccgctaag aggcctttgc tgcgactccc atgttcgggt
    26641 cgcagcatcg cacactctcc aacatgaaat ggctaaatgg attattgttg cgtaggaagg
    26701 aacaataatg atagtgttcc cagagaagga acgctactga gagtaaagac actaatgaaa
    26761 atggccaatc atccccgccc gggggacatt attcaggaat cactggacga acttaatgtc
    26821 agcctgcgcg agtttgccag agcaatggaa attgctccct caacggcaag ccgattgctg
    26881 accggaaaag cagctttgac gccagaaatg gcaattaaac tttccgtggt gatcggcagt
    26941 tcgccgcaaa tgtggctgaa tctgcaaaac gcctggagtc tggcagaggc tgcaaaaacg
    27001 gtggatgtgt cgcgattacg ccgtctggtt gcgcaataaa aaatggcgcc ttttacagcg
    27061 ccatttgaaa ctgatgacaa acgcaacatt gcctgatgcg ctacgcttat caggcctaca
    27121 cgttcactgc aatctattgg atttgaacgg tttgtaggcc ggataaggcg ttcacgccgc
    27181 atccggcatg aacaaagcct acgttgtcta caatatgaaa atggcgcctt ttacagcgcc
    27241 attttcagct taaagttcaa aagagattat atcccttctt cactctcttt tttcgcttct
    27301 gccttctcga tttcacgata ccagcgcgga tggtgtttct tcgcccagcg acggctcacc
    27361 ttcccttcga tcatcccttt aatcgatcct ttcacccaga atgccatata catatggatc
    27421 aggatggcgt ggatcaggat gatacccgca gccgcgtgga tcagcaggct gtagcgaaca
    27481 acctgcatcg ggaagtactg cgcaaagtac ggacgccaga taatcacccc ggtcaccagc
    27541 agcacgaaaa tcatgctcat gatcgaccag aacatcatct tttgcccggc gttgtactta
    27601 ccgacatccg ccactttatg ctcattgcct ttcaatactt cgacaatgtt taacagccac
    27661 ggaatatctt tcttatccgg gatgttgtga tgcacaaaac gcacaaacat aaacatcagt
    27721 gcgacgaaaa tcgcaatgcc gaagaatggg tgcaaaatgc gtcccatctg cggcgtaccg
    27781 aaggtttgcg tcagccattg cagcgtcggg aagaagaacg aaatcccgga cagcgccacc
    27841 aggaagaagc aaatcaccac ggtccagtga caggcgcgat caataaattt ggtgcgcaca
    27901 atcattttcg acttactcat gatgatcctc ctcgtcatcg tccacttcct tattcgggcc
    27961 aataccgatg tagtggaaaa tcaaccctgc aaaagtggca ataaagccag ccgctgccag
    28021 cggtttcaac gcgcctttcc acaggcttac cgaggtgtcg atcttcggat ctttcggcag
    28081 accgtgatac agctccggct gatcggcgtg atgcagcacg tacataacgt gcgtaccacc
    28141 gaccccttcc gggttgtaga cgccagcatg ttcgtaacca cgcgctttca gtttcgccac
    28201 gcgctgttcc gccagctcca gcatctcctt cttggtgccg aagtggatag ccccggtcgg
    28261 acaggttttc acacaagccg gttcctggcc gacgctgacg cgatcgacgc agagcgtgca
    28321 tttatatacc cggttatcct ctttgttgag gcgcggaata ttaaacggac acccggcaat
    28381 gcagtaacca cagccgatgc agttttccga ctggaaatcg acaatcccgt tagcgtactg
    28441 aatgattgca ccggcagacg ggcacgcctt caggcagccc ggatcttcac agtgcataca
    28501 gccatcttta cggatcagcc actccagctt gccgctctgt tcggtttcgc taaagcgcat
    28561 caccgtccag gacttggcgc tcagatcggc ggggttatcg taaaccccga cgcagtgccc
    28621 cacttcatca cggatgtcgt tccactccga acacgccacc tgacaggctt tacagccgat
    28681 acaggtggaa acgtcgataa gttttgcgac ttctgctttg taatcacgca cctgagaagg
    28741 cggcgtgatg gagttagttg cggacctttt gataatgtcc tgcgtttcca tagccatctg
    28801 ttcgccccct tacgccttct cgatgttgac taagaacgct ttatattccg gcgtttgcga
    28861 gtttgcatca ccgacattcg gcgtcagagt gttagctata taacctttac gcgcgacacc
    28921 ctcaaagccc cagtggattg gaatacccac cgtttcaacc tgctgaccat ttacattcag
    28981 cggtttcaga cgacgcgtta ccacagccac cgcgcggata aagccacgct tgcttgagac
    29041 agtgacacga tcgccattat taatgccttt cgccgccgcc agcgtttcgc tgatttccac
    29101 aaactgttcc ggctgagcaa ttgcgttgag caacgcgtgc ttggtccagg tgtggaagtg
    29161 ctcggtcaga cgataggtcg tacccacata cgggaactgc tcttttttac ccatccgcag
    29221 cgcgtcttgt tcatacagac gaaccaccgg gttagaaacc acgttcggat gcagcgggtt
    29281 agttcccagc ggcgtttcaa ttggctcgta gtgttccggg aacggacctt ccgccatttt
    29341 gttgatggca aacaggcgtc ccatcccttc cggctgcatg ataaacggcc cggttggcgt
    29401 acccggtgcg gcattgccga agtcagggat atcgttaccc gtccacttgc tgccgttcca
    29461 ctggatcagc atccgtttcg gatcccacgg tttaccgttg atatctgccg aagcacggtt
    29521 gtagagtacg cgacggttaa gcggccacgc ccaggcccat cccagcgtat tccccagacc
    29581 ggacgggtcg gagttatcgc ggttagccat ctggttgccc tgctctgtcc agctaccggt
    29641 gtagatccag caagaagatg cggttgtacc gtcatcacgc agatgcgcaa agctactcag
    29701 caactgacct ttcttcgcaa tcagcacgcc attagcatca tagagatctt ccagcgcata
    29761 gccgttgttc tctttagcta cttcgtcaga ttgcggttcg tgcggctgct tgtagttcca
    29821 gctcatcttc atcagcggtt ctacgccttt accaccttca gcctggtaca gctcgcgcag
    29881 atgatggtag atacccgcca gaatttcgcc gtcgttacgc gcttcgcccg gcgcgtcctg
    29941 acctttccag tgccactgca accagcgacc ggagttagcg atagaaccat cttcttctgc
    30001 aaagcaggtc gaaggcagac ggaatacttc agtctgaata gacgccggat cgacatcgtt
    30061 cgactcgccg tggttctgcc agaaggtaga ggtttcagtc accagcggat cgataaccac
    30121 catgtacttc agcttgctca ggcagctcac cactttgttt ttgtccggga aggacgcaac
    30181 cgggttaaag ccctggcaga aataaccggt gactttgcct tcatccatca tgttgaaata
    30241 cttgatgacg tcgtaggtct ggtcccactt cggcagccag tcatagcccc agttgttctc
    30301 tttctgcgcg gcatcgccat agaaagattt catcaggcta acgaagaact tcggatagtt
    30361 gctccagtag ttcacctgac cagccagcgt cgctttcggc gtgttcgctt ccagatacga
    30421 ctgcaaatca acctgttttt ctgacggcag cgtcagataa cctggcaggc tggtagagag
    30481 cagacctaag tcagtcagac cctgaatgtt ggagtgacca cgcaatgcat tcacgccgcc
    30541 accggccata cccatgttac cgagcagcaa ctggatcatc gccatagtac ggatgttctg
    30601 cgcacccacg gtgtgctgcg tccagcccag cgcgtacagg aaggtggttg tgcgatccgg
    30661 tgcgctggtg gaggccagca cttcacacac tttcaggaag tcggcttttg gcgtaccaca
    30721 gatgttttca acaacgtccg gcgtgtagcg ggaaacgtgc tctttcagca ggttccacac
    30781 acagcgcgga tgagtcagtg tttcatcgcg ttttgcatag ccgttttcat cgaactgata
    30841 gttccaggac gacttatcgt actggcgttt ttcagcgtcg tagccgctga acagaccgtc
    30901 ttcgaaagca aaatcatcac gcaccagcag gctggcgttg gtgtaatgct taacgtattc
    30961 ggcgttgatt ttgttgtttt cgatcaggta gcgcaaaacg ccagacagga acgtaatgtc
    31021 cgtaccggaa cgaataggcg cgtagatatc cgccacagaa gcggtacgcg taaaacgggg
    31081 atcgacaacg atcaaggttg catcgttgtt gtttttcgct tccatcgccc agcggaaacc
    31141 gacgggatgc gcttcagcag cgttaccgcc cattaccatt acgacgttag cgtttttgat
    31201 atccacccag tggttggtca tcgcaccgcg accaaatgtt ggagcaagac ttgctaccgt
    31261 tggtccgtgt cagacgcgcg cctggttgtc taccgccagc atcccgaggg agcgggcaaa
    31321 tttctgggtc agcatcccgg tttcgttgct ggcaccggaa gcacacagca taccggtaga
    31381 aagccaacgg tttaccgtta cgccctgctc gttcttttca ataaagttag cgtcacggtc
    31441 agctttcatc agcttcgcaa tacgggagaa tgcttcttcc cagctaatgc gctgccattt
    31501 gtcagaacct ggcgcacgat attccgggta gcgcagacgg ttttcgctgt tgacgtaatc
    31561 cagcaaaccg gcccctttcg ggcacagcgc cccacggctt accggatgat ccgggtcacc
    31621 ttcaatgtga taaatcgctt ctctggcgtt tttcgcgcca tcacccaggc tatacatcaa
    31681 tagcccgcaa cctacggaac agtatgtgca ggtgttacgg atctctttag cgcgtaataa
    31741 tttgtagttt cgcgcctgag ccagtgcttg cttcggggca aagcccaatg ccgctactgt
    31801 tgttccagcc ataccgcccg cgcagatttt aaaaaattgt ctgcgactga cgtccattgc
    31861 tttcctcttt tttcaggggt attactgcgc gaggaaacta acagtaaaat tctgaatctt
    31921 tttgcgcgaa atcaaaaaga acacgtgttt gccacaaaat aatccgcctg cgcaaattga
    31981 cctacctcaa tagtggtaga aaaacgcacc actgcctgac aggccagtta aaaaaatgct
    32041 ataaaattca gcttaatttt taacggcaag agagacaaaa cagcgagcat gacacgacaa
    32101 aaagcaacgc tcatagggct aatagcgatc gtcctgtgga gcacgatggt aggattgatt
    32161 cgcggtgtca gtgaggggct cggcccggtc ggtggcgcag ctgctatcta ttcattaagc
    32221 gggctgctgt taatcttcac ggttggattt ccgcgtattc ggcaaatccc gaaaggctat
    32281 ttactcgccg ggagtctgtt attcgtcagc tatgaaatct gtctggcgct ttccttaggg
    32341 tatgcggcga cccgtcatca ggcgattgaa gtgggtatgg tgaactatct gtggcccagc
    32401 ctgacaattc tctttgccat tctgtttaat ggtcagaaaa ccaactggct gattgtacca
    32461 ggattattat tagccctcgt cggcgtctgc tgggtgttag gcggtgacaa tgggttacat
    32521 tatgatgaaa tcatcaataa tatcaccacc agcccattga gttatttcct ggcgttcatt
    32581 ggtgcgttta tctgggcagc ctactgcaca gtaacgaata aatacgcacg cggatttaac
    32641 ggaattaccg tttttgtcct gctaacggga gcaagtctgt gggtttacta ttttctcacg
    32701 ccacaaccag aaatggtatt tagcacgcca gtcatgatta aactcatctc tgcggcattt
    32761 accttaggat ttgcttatgc tgcatggaat gtcggtatac tgcatggcaa cgtcaccata
    32821 atggcggtag gttcgtattt tacgcccgta ctttcctcag cgcttgcagc cgtgctgctc
    32881 agcgccccgc tgtcgttctc gttctggcaa ggcgcgctga tggtctgcgg cggttccctg
    32941 ctctgctggc tggcgacacg tcgtggctaa aataattgcc gggtctacgg cccggcaatt
    33001 aatcagataa accccgacaa tattaaaaaa taaatttc
//
LOCUS       DAZFAB010000044        32710 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZFAB010000044 DAZFAB010000000
VERSION     DAZFAB010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.044"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            139..3891
                     /gene="yfaL"
                     /locus_tag="ACHJWA_002387"
     CDS             139..3891
                     /gene="yfaL"
                     /locus_tag="ACHJWA_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFO4308973.1"
                     /translation="MRVIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGITQVWSIADGQWLVFSDMTNNASGGAVFLQQGAEFTLSPENETGMTLFAN
                     NTVSGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAIDLRVTNA
                     VFRNNIANDGKGGAIYTINNDIYLSDDVFNNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPSGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSSAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQN
                     QAELNVGSTQQTFAHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSY
                     VLAGAQSMALTGDIVVDAGAVLSLEGDAADLAALQDDPQSIVLNGGMLDLSDFSTWQS
                     GTSYKDGLEVSGSSGTVIGSQDVVDLAGGNDMHIGGDGKDGVYVVIDAGDGQVSLAND
                     NQYLGTTQIASGTLMVSDNSQLGYTHYNRQVIFTDKPQESVMEITANVDTRSTTTEHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTLTKTGAGTLELTASGTTQSA
                     VRVEEGTLQGDVADIFPYASSLWVGDGATFVTGADQDIQSIDATSSGTIDISDGTVLR
                     LTGQDTSVALNASLFNCDGTLVNATDGVTLTGELNTNLETDSLTYLSNVTVNGNLTNT
                     SGAVSLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVMNGNTAGNTTVVVN
                     SITGIGEPTSTGIKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPSPPDPDPTPDPDPTQDPDPTPDPEPTPAYQPVLNAKVGGYLNNLRAANQAF
                     MMERRDHAGGDGQTLNLRVIGGDYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGTDGEW
                     MLGIVGGYSDNQGDSRSSMTGTRADNQNHGYAVGLTSSWFQHGKQKQGAWLDNWLQYA
                     WFSNDVSEHEDGVDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAA
                     NRARVSQSQGDDIQTRLGLHSEWRTAVHVIPTLDLNYYHDPHSTEIEEDASTISDDAV
                     KQRGEIKVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(4032..4754)
                     /gene="ubiG"
                     /locus_tag="ACHJWA_002388"
     CDS             complement(4032..4754)
                     /gene="ubiG"
                     /locus_tag="ACHJWA_002388"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFO4308974.1"
                     /translation="MNAEKSPVNHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LSYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNTFKLGPGVDVNYMLHTQNK"
     gene            4901..7528
                     /gene="gyrA"
                     /locus_tag="ACHJWA_002389"
     CDS             4901..7528
                     /gene="gyrA"
                     /locus_tag="ACHJWA_002389"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFO4308975.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7736..9364
                     /gene="yfaA"
                     /locus_tag="ACHJWA_002390"
     CDS             7736..9364
                     /gene="yfaA"
                     /locus_tag="ACHJWA_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFO4308976.1"
                     /translation="MALLSAGVWALQYAGSGPEKTLSPLVVHNNLQIDLNEPDLFLDS
                     DSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIEGSIRRIVYEHDLTLKDKLFS
                     SLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPLLFAATSDSQLSKTEISSIKI
                     NSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFKDDQQDTEATAIASDLLSGKK
                     RWQASFGLEERAAEKTPVRQRIVASARLLGFGYQRLMPSFAGVRFEMGNDGWHSFVAL
                     NDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAEEMLSHISQENDKLNGALDGA
                     AGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNIGAHESKAPEGVLPVSQTQQG
                     EAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLAMQNKTLLFSLDDTLVNNALQ
                     TLNKTRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLTSLPKNLEPVFYNAAQTLLMP
                     KLDALSQQPRYVMKLAQMEPGAAWQWLPIIWQPL"
     gene            9361..9984
                     /gene="yfaT"
                     /locus_tag="ACHJWA_002391"
     CDS             9361..9984
                     /gene="yfaT"
                     /locus_tag="ACHJWA_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HFO4308977.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            10008..14522
                     /locus_tag="ACHJWA_002392"
     CDS             10008..14522
                     /locus_tag="ACHJWA_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HFO4308978.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEESKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELQLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGKELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVDVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     NARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGDLPSGGKSFTVNFDKPGNYNLTLRDKDGLILAGLSHAVSGKGSM
                     SHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKPSAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLAQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGDNALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKTEDFSEEDTRDINDSLILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAVNSNQPLAHTVVLLNSGGDATQAAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPSVVLPAPAGAWAKHKLTGGGEYWRWVGQGVP
                     DILSFGDELSPQNVQVRWREAAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAGKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            14523..16172
                     /gene="yfaQ"
                     /locus_tag="ACHJWA_002393"
                     /pseudo
     CDS             14523..16172
                     /gene="yfaQ"
                     /locus_tag="ACHJWA_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
     gene            16177..16953
                     /gene="yfaP"
                     /locus_tag="ACHJWA_002394"
     CDS             16177..16953
                     /gene="yfaP"
                     /locus_tag="ACHJWA_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFO4308979.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            17228..20077
                     /gene="rcsC"
                     /locus_tag="ACHJWA_002395"
     CDS             17228..20077
                     /gene="rcsC"
                     /locus_tag="ACHJWA_002395"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFO4308980.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKADPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLMIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(20277..20927)
                     /gene="rcsB"
                     /locus_tag="ACHJWA_002396"
     CDS             complement(20277..20927)
                     /gene="rcsB"
                     /locus_tag="ACHJWA_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFO4308981.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(20944..23616)
                     /gene="rcsD"
                     /locus_tag="ACHJWA_002397"
     CDS             complement(20944..23616)
                     /gene="rcsD"
                     /locus_tag="ACHJWA_002397"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFO4308982.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            24355..25458
                     /gene="ompC"
                     /locus_tag="ACHJWA_002398"
     CDS             24355..25458
                     /gene="ompC"
                     /locus_tag="ACHJWA_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO4308983.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVSGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQNF
                     EAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFNKNMSTY
                     VDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            25570..26625
                     /gene="apbE"
                     /locus_tag="ACHJWA_002399"
     CDS             25570..26625
                     /gene="apbE"
                     /locus_tag="ACHJWA_002399"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFO4308984.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            26699..27763
                     /gene="ada"
                     /locus_tag="ACHJWA_002400"
     CDS             26699..27763
                     /gene="ada"
                     /locus_tag="ACHJWA_002400"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFO4308985.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYHKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            27763..28413
                     /gene="alkB"
                     /locus_tag="ACHJWA_002401"
     CDS             27763..28413
                     /gene="alkB"
                     /locus_tag="ACHJWA_002401"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFO4308986.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHNLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            28489..30132
                     /gene="yojI"
                     /locus_tag="ACHJWA_002402"
     CDS             28489..30132
                     /gene="yojI"
                     /locus_tag="ACHJWA_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFO4308987.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPSWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELRNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            30350..31996
                     /gene="mqo"
                     /locus_tag="ACHJWA_002403"
     CDS             30350..31996
                     /gene="mqo"
                     /locus_tag="ACHJWA_002403"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFO4308988.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLNLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(32146..32634)
                     /gene="eco"
                     /locus_tag="ACHJWA_002404"
     CDS             complement(32146..32634)
                     /gene="eco"
                     /locus_tag="ACHJWA_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFO4308989.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDTGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
BASE COUNT         7971 a         8008 c         8871 g         7860 t
ORIGIN      
        1 atgacgcact gatactattc atcaaataat aacaaaatag ctattgcatc gggttggaca
       61 atttacaaaa gaaagataac ccatattgta taatgcgttt aaatatgccg tcttatataa
      121 aatgatggtc agattaatat gcgggttatc tttctacgca aggagtattt atctctactc
      181 ccgtcaatga ttgcatctct tttctctgct aacggtgtcg cggcggccat tgatttatgc
      241 cagggatatg atatcaaagc gagttgtcac gccagcaggc aaagcctttc aggcattacg
      301 caggtctgga gtattgccga tgggcaatgg ctggtttttt cggatatgac caataatgcc
      361 agcggtgggg ccgtattttt gcaacaagga gcggaattta cattatcacc agaaaatgaa
      421 actggaatga ctctgtttgc caataacacc gtttcaggag aatataataa cggcggggca
      481 atatttgcta aagaaaactc aacgctgaat cttacggatg ttattttttc tggtaacgtc
      541 gcaggcggct atggtggcgc aatctattct tctggtacta acgataccgg tgccatcgat
      601 ttacgtgtca ctaacgccgt gtttcgcaat aacatcgcta atgacggcaa aggtggtgca
      661 atttatacca tcaataatga tatctattta agtgatgatg tttttaacaa taaccaggca
      721 tatacatcaa caagttacag tgatggcgat ggcggcgcaa tcgatgtcac agataataat
      781 agcgacagca agcatccttc aggttatacg ataataaata acactgcctt tacaaataac
      841 actgccgaag gttatggcgg ggcgatatat accaatagcg cgacggctcc ctatcttatt
      901 gatatttctg ttgatgacag ctacagccag aacggaggcg tgttagtcga tgagaacaat
      961 agcgcagcag gctatggaga tggtccttcc tctgcggcgg gtggctttat gtatctcggc
     1021 ttaagtgaag ttacctttga tattgccgac ggaaaaacgc tggttattgg caatacagag
     1081 aatgacggag ctgttgactc tattgctggt accgggttaa tcaccaaaac aggttccggc
     1141 gatctggtac ttaatgcaga taacaatgac tttactggtg agatgcagat tgaaaacggt
     1201 gaagttaccc tgggccgcag caactccctg atgaatgtcg gcgatacgca ttgccaggac
     1261 gatccgcaag actgctacgg tctgacgata gggagtattg ataagtacca gaatcaggca
     1321 gagctaaatg ttggctccac ccaacaaacc tttgcgcact cattgacggg ctttcagaat
     1381 ggcactttaa atatcgatgc tggtggcaat gttactgtta atcaaggcag ttttgctggc
     1441 accatcgaag gtgctggtca gctcaccatt gcgcaaaacg gcagctatgt gctggcgggg
     1501 gcgcagtcga tggcgctaac cggcgatata gtggtggatg ctggtgcggt gctttcgctg
     1561 gaaggcgacg cggcagatct tgccgctctc caggacgatc cgcagtcgat cgtgttaaac
     1621 ggcggtatgc tcgatctctc tgatttctcc acctggcaga gcggtacatc atacaaagat
     1681 ggccttgaag tcagtggcag cagcggaacg gttatcggca gtcaggatgt ggtagatctt
     1741 gcaggcggaa acgatatgca tatcggcggc gacgggaaag atggcgtcta cgtggtgatc
     1801 gatgcgggtg acgggcaggt cagcctggca aatgacaatc aatacctcgg cacaacgcaa
     1861 atcgcttccg gtacgctgat ggtgagcgac aactcgcagc ttggatatac ccattataac
     1921 cgccaggtta tctttaccga taagccacaa gaaagcgtga tggagattac tgccaatgtc
     1981 gatactcgct ctacaacgac tgagcatggg cgtgatattg aaatgcgcgc cgacggtgaa
     2041 gtggcagttg atgcgggggt agacacgcag tggggcgcac tgatggctga cagcagcggg
     2101 cagcatcagg atgagggtag cacattgact aaaacggggg cgggtacact ggagctgacc
     2161 gccagcggta caacgcagtc agcggtgaga gtagaagagg gcacgctgca aggtgatgtt
     2221 gcggatatct tcccttatgc ttcgtcgcta tgggtcggtg acggggcaac gttcgttact
     2281 ggcgcggatc aggatattca gtcaattgat gctacttcca gcggcactat cgacatcagc
     2341 gatggtacgg ttttgcgcct gaccgggcag gatacttccg tcgcccttaa tgcctcactg
     2401 tttaactgcg atgggacgct ggtgaatgcc accgatggtg tgacgttgac aggtgagctt
     2461 aataccaacc ttgaaactga cagcctgact tatctttcca acgtgacggt taatggcaat
     2521 ctgaccaata cgtccggtgc ggttagcctg caaaatggcg tcgctggcga tacgctgacg
     2581 gtaaacggtg attataccgg cggcggtacg ctactgctcg atagcgaatt aaacggcgat
     2641 gactcggtaa gcgatcaatt ggtgatgaac ggtaatactg ctggcaacac aactgtggtg
     2701 gttaactcca ttacagggat tggtgagccg acatcgacag gcattaaagt ggttgatttc
     2761 gcagctgatc ccacgcagtt tcaaaacaat gcgcagttca gtctggcagg cagcggctac
     2821 gtcaatatgg gagcgtatga ctacacgctg gtggaagata acaacgactg gtatctgcga
     2881 tcgcaagaag taacgccgcc atcgccacct gatccagacc cgactcccga tcctgatccc
     2941 acgcaggatc ctgatccaac acccgacccg gaacctacgc ctgcttacca gccggtgttg
     3001 aatgccaaag ttggcggtta tctcaataac ctgcgggcgg caaatcaggc gtttatgatg
     3061 gagcgacgcg atcacgcagg tggcgatggt cagacgctga atttacgtgt tatcggcgga
     3121 gattatcatt acacagcagc ggggcaactg gctcaacatg aagacacttc tacggtgcaa
     3181 cttagcggcg atctgtttag cgggcgctgg ggcacggatg gcgagtggat gcttgggatt
     3241 gttggtggct acagcgataa ccagggcgac agccgctcga gtatgaccgg aactcgcgcc
     3301 gataaccaga accacggtta tgcggttggg ctgacctcaa gctggtttca gcacggtaag
     3361 cagaagcagg gggcctggct ggataactgg ttgcagtacg cgtggtttag caatgatgtt
     3421 tctgaacatg aagatggcgt ggatcattac cattcgtcgg ggattatcgc ctcgctggaa
     3481 gcggggtatc agtggttacc ggggcgtggt gtggtgattg aaccgcaggc gcaggtgatt
     3541 tatcagggcg tgcagcagga tgattttacc gccgctaacc gtgcgcgcgt gtcacaatcg
     3601 cagggtgatg atattcagac gcggctgggt ttacacagcg aatggcgtac cgctgttcat
     3661 gtcataccaa cattagatct gaattattat cacgatcccc attcgacgga aattgaagaa
     3721 gatgccagca ctatcagtga cgatgcggtg aagcaacggg gtgaaataaa agtgggagtc
     3781 acgggcaata tcagtcagcg agtttcgctg cgtggtagcg tggcgtggca gaaagggagt
     3841 gatgattttg cccagacggc agggtttttg tcgatgacgg tgaaatggta atcgctgtct
     3901 gaccatcact attgctggat gcggcgtaaa cgcctaatca ggcctacgga gagcatttgt
     3961 aggcctgata agacgcggca agcgtcgcat caggcattgg cgcggttgcc ggatgaatcc
     4021 aaacaacaac ctcacttatt ctgcgtgtgc agcatatagt tcacatccac gccggggccg
     4081 agtttaaaag tattagtgat cgggttgtaa tgtagcccgg ttatatgtcg ctctttaagg
     4141 cttgtctgat ctacccagcc cagcagttct gccggtttaa taaacttctt cacatcatgt
     4201 gtacctttag gcaccatgcg caaaatatat tccgcgccga ctaccgccat cagccatgac
     4261 ttgccgttgc ggttaagcgt cgagaaaaag acatcgccgc ccggtttcac cagttgcgca
     4321 caggctctga ccactgactg cggatcaggg acgtgctcca gcatctccat gcaggtcacc
     4381 acatcatact gcccggcatg ttttgccgcg tgctcttcca cggtttcctg cacgtaatcc
     4441 acctgaatgc cgctttccag cgcgtgcagc tttgccacct gcaatggctc aaagcccata
     4501 tccagaccgg tcaccgtcgc gccttcgcgc gccatactct cggccagaat gccgccgcca
     4561 caaccgacat cgagcacctt tttgccaaat aagccgccag cacgctcggc aatatagctc
     4621 agacgcagcg ggttaatgcg gtgcagcggt ttgaactcac cttccagatc ccaccagcgg
     4681 gaggcgacgg cttcaaattt agcgatctct tcgtggtcta cgttatggtt taccggcgat
     4741 ttttcggcat tcattggcac ttctactccg taattggcaa gacaaacgag tatatcaggc
     4801 attggatgtg aataaagcgt ataggtttac ctcaatctgc gcggctgtgt tataatttgc
     4861 gacctttgaa tccgggatac agtagaggga tagcggttag atgagcgacc ttgcgagaga
     4921 aattacaccg gtcaacattg aggaagagct gaagagctcc tatctggatt atgcgatgtc
     4981 ggtcattgtt ggccgtgcgc tgccagatgt ccgagatggc ctgaagccgg tacaccgtcg
     5041 cgtactttac gccatgaacg tactaggcaa tgactggaac aaagcctata aaaaatctgc
     5101 ccgtgtcgtt ggtgacgtaa tcggtaaata ccatccccat ggtgactcgg cagtttatga
     5161 cacgatcgtc cgtatggcgc agccattctc gctgcgttac atgctggtag acggtcaggg
     5221 taacttcggt tccatcgacg gcgactctgc ggcggcaatg cgttatacgg aaatccgtct
     5281 ggcgaaaatt gcccatgaac tgatggccga tctcgaaaaa gagacggtcg atttcgttga
     5341 taactatgac ggcacggaaa aaattcccga cgtcatgcca accaaaattc ctaacctgct
     5401 ggtgaacggt tcttccggta tcgccgtagg tatggcaacc aacatcccgc cgcacaacct
     5461 gacggaagtc atcaacggtt gtctggcgta tatcgatgat gaagacatca gcattgaagg
     5521 gctgatggaa cacatcccgg ggccggactt cccgacggcg gcaatcatta acggtcgtcg
     5581 cggtattgaa gaagcttacc gtaccggtcg cggcaaggta tatatccgcg cccgtgctga
     5641 agtggaagtt gacgccaaaa ccggacgtga aaccattatc gtccacgaaa ttccgtatca
     5701 ggtaaacaaa gcgcgcctga tcgagaagat tgcggaactg gtaaaagaaa aacgcgtgga
     5761 aggcatcagc gcgctgcgtg acgagtctga caaagacggt atgcgcatcg tgattgaagt
     5821 gaaacgcgat gcggtcggtg aagttgtgct caacaacctc tactcccaga cccagttgca
     5881 ggtttctttc ggtatcaaca tggtggcatt gcaccatggt cagccaaaga tcatgaacct
     5941 gaaagacatc atcgcggcgt ttgttcgtca ccgccgtgaa gtggtgaccc gtcgtactat
     6001 tttcgaactg cgtaaagctc gcgatcgtgc tcatatcctt gaagcattag ccgtggcgct
     6061 ggcgaacatc gacccgatca tcgaactgat ccgtcatgcg ccgacgcctg cagaagcgaa
     6121 aactgcgctg gtggctaatc cgtggcagct gggcaacgtt gccgcgatgc tcgaacgtgc
     6181 tggcgacgat gctgcgcgtc cggaatggct ggagccagag ttcggcgtgc gtgatggtct
     6241 gtactacctg accgaacagc aagctcaggc aattctggat ctgcgtttgc agaaactgac
     6301 cggccttgag cacgaaaaac tgctcgacga atacaaagag ctgctggatc agatcgcgga
     6361 actgctgcgt attcttggta gcgccgatcg tctgatggaa gtgatccgtg aagagctgga
     6421 gctggttcgt gaacagttcg gtgacaaacg tcgtactgaa atcaccgcca acagcgcaga
     6481 catcaacctg gaagatctga tcacccagga agatgtggtc gtgacgctct ctcaccaggg
     6541 ctacgttaaa tatcagccgc tttctgaata cgaagcgcag cgtcgtggcg ggaaaggtaa
     6601 atctgccgca cgtattaaag aagaagactt tatcgaccga ctgctggtgg cgaacacgca
     6661 cgaccatatt ctgtgcttct ccagccgtgg tcgcgtctat tcgatgaaag tttaccagtt
     6721 gccggaggcc actcgtggcg cgcgcggtcg tccgatcgtc aacctgctgc cgctggagca
     6781 ggacgaacgt atcaccgcga tcctgccggt gaccgagttt gaagaaggcg tgaaagtctt
     6841 catggcgacc gctaacggta ctgtgaagaa aaccgtcctc accgagttca accgtctgcg
     6901 taccgccggt aaagtggcga tcaaactggt tgaaggcgat gagctgatcg gcgttgacct
     6961 gaccagcggc gaagacgaag taatgctgtt ctccgccgaa ggtaaagtgg tgcgctttaa
     7021 agagtcttct gtccgtgcga tgggctgcaa caccaccggt gttcgcggta ttcgtttagg
     7081 cgaaggtgat aaagtcgtct ctctgatcgt gcctcgtggc gatggcgcaa tcctcaccgc
     7141 aacgcagaac ggttatggta aacgtaccgc agtggcggaa tacccgacta agtcgcgtgc
     7201 gacgaaaggg gttatctcca tcaaggttac cgaacgtaac ggtttagttg ttggcgcggt
     7261 acaggtagat gactgcgacc agatcatgat gatcaccgat gccggtacgc tggtacgtac
     7321 tcgcgtttca gaaatcagca tcgtgggccg taacacccag ggcgtgatcc tcatccgtac
     7381 tgcggaagat gaaaacgtag tgggtctgca acgtgttgct gaaccggttg acgaggaaga
     7441 tctggatacc atcgacggca gtgccgcgga aggggacgat gaaatcgctc cggaagtgga
     7501 cgttgacgac gagccagaag aagaataatt ttacttcttc atgccaaaag ggagccatct
     7561 cccttttttg aattgaaaag tccaggctgc aaagtctggg cttttgtcgt attagggcgc
     7621 ggtaaagttt ggctgtgtcc gtaaaaaatg gctggctata cacaaggaat gtggcaatga
     7681 gtggtgaaaa aaagcgaaag gctggcggtt ctatggtctt gtaggttttg gcgcaatagc
     7741 actgctttcc gctggcgtct gggcgttgca atatgctggc agtgggccag aaaaaacgtt
     7801 gtcgccgctg gtggtgcaca ataatttgca aattgatctc aatgagccgg acctctttct
     7861 cgacagcgat tctctaagcc agcttcccaa agatctcctc accattccgt ttctccacga
     7921 tgttctgagt gaagatttcg ttttctatta tcagaatcat gccgatcgtc tgggcattga
     7981 aggcagcatt cgtcgcattg tctatgaaca cgatctcacg ctgaaagata agctcttttc
     8041 gtcactctta gatcagcccg cgcaggcggc gctatggcac gataaacaag gccatctttc
     8101 acattacatg gtgctgatcc aacgcagtgg tttaagcaaa ctgctggagc cattgttatt
     8161 tgccgctacc agcgacagcc agttaagcaa aacggaaatc agtagcatca agataaatag
     8221 tgaaactatt cctgtttatc agttgcgcta taacggcaat aacgccctga tgttcgcgac
     8281 atatcaggac aagatgctgg tgttttccag cacggatatg ctgtttaaag atgatcagca
     8341 ggataccgaa gccacggcga tcgcaagtga tttgttgagc ggcaaaaaac gctggcaagc
     8401 cagctttggc ctggaagagc gtgctgccga aaaaacgcca gtacgccagc gcatcgtggc
     8461 cagcgccagg ttgctgggtt ttggctacca gcggttaatg ccttcttttg ctggcgtacg
     8521 cttcgaaatg ggtaacgacg gctggcacag ttttgtggcg ttaaatgatg aatccgccag
     8581 cgtagatgcc agtttcgatt ttacgccggt ctggaacagt atgcctgccg gggccagctt
     8641 ctgtgtggcg gtgccgtatt cacacggtat tgccgaagag atgctttcgc acatcagcca
     8701 ggaaaacgac aagttgaatg gggcgttgga cggtgccgcg gggctgtgct ggtatgaaga
     8761 ctcaaaattg caaaccccgc tgtttgtcgg tcagtttgat ggcactgccg aacaggcgca
     8821 attgccaggg aaactgttta cgcaaaatat tggtgcgcac gaaagcaaag cgccagaagg
     8881 tgttttgccg gtaagccaga ctcagcaggg cgaagcgcaa atctggcgtc gcgaagtgag
     8941 ttcccgatac ggtcagtatc cgaaagcgca ggcggcgcaa cccgatcaat taatgtcgga
     9001 ttattttttc cgcgtgtcgc tggcgatgca aaacaaaacg ctgcttttct ccctcgatga
     9061 cacgctggtt aataacgccc tgcaaacact gaataaaacc cgcccggcaa tggtggatgt
     9121 aatacccact gatggcatcg ttccgctcta tatcaatcca caaggcgtgg cgaaactgct
     9181 gcgtaacgaa acgttgacca gtctgccgaa gaatctcgaa ccggtttttt ataacgccgc
     9241 acaaacttta ttaatgccga agctggacgc tttatctcaa caaccgcgtt atgtcatgaa
     9301 gctggcccag atggaacccg gtgccgcctg gcagtggcta cccataatct ggcagccgct
     9361 atgaggcacg ggctgctggc gctgatttgc tggctgtgtt gtgttgttgc ccatagcgaa
     9421 atgctgaatg tcgaacaatc cgggctgttt cgcgcctggt ttgtacgcat tgcacaagaa
     9481 cagctccgcc aggggccaag tccacgctgg tatcagcagg attgtgcggg cctggtgcga
     9541 tttgcggcga acgagacgct gaaagttcac gacagtaaat ggctaaaaag taacggttta
     9601 tctagccagt atttgccgcc agagatgacg ctaacacctg aacagcgtca actggcgcaa
     9661 aactggaatc aggggaacgg gaaaaccggc ccctacgtga ccgcgattaa tttgattcag
     9721 tacaacagcc agtttattgg ccaggacata aaccaggcgc tgcctggcga tatgattttt
     9781 ttcgatcagg gcgatgccca gcacttaatg gtctggatgg ggcgttacgt catctaccac
     9841 accggaagcg ccacgaaaac tgacaacgga atgcgcgcag tcagtctgca acaacttatg
     9901 acatggaagg acacccgatg gatacccaac gattccaatc ccaatttcat tggcatttat
     9961 cgtttaaatt ttctggcgcg atagcagcgt gcttgtccct gtctcttgtg ggaacagggc
    10021 ttgctaatgc tgatgattcg cttccttcca gtaactatgc gccgcccgcc gggggaacat
    10081 tctttttgct tgctgacagc agttttagca gcagtgaaga gtcgaaagtg cgactggaag
    10141 cgccggggcg tgattatcgg cgctatcaga tggaagagta cggcggcgtg gacgttcgcc
    10201 tgtatcgtat tcctgacccg atggcatttt tgcgccagca gaaaaacctg catcgcattg
    10261 tggtgcaacc gcaatatctg ggcgacgggc tgaacaatac gctgacctgg ctgtgggata
    10321 actggtacgg caaatctcgc cgcgtgatgc agcgtacttt ctcttctcag tcacggcaga
    10381 atgtgactca ggcattaccc gaattacagc tcggcaatgc cattattaaa ccttcccgtt
    10441 atgtacagaa caaccagttt tccccgctga aaaaatatcc cctggtggaa cagttccgtt
    10501 atccactatg gcaggctaaa ccggtcgagc cgcagcaagg ggtaaaactg gaaggcgcat
    10561 ccagcaattt catctcgccg cagccgggta acatttatat tcctctcggc caacaagagc
    10621 cgggactgta cctcgtcgag gcgatggttg gtgggtatcg ggcgacgacg gtggtgtttg
    10681 tttccgatac cgtggcgctt agcaaagtgt caggcaaaga gcttctggtg tggaccgcgg
    10741 gtaaaaaaca gggtgaagcg aagcccggct cagagatctt gtggactgac ggtcttggcg
    10801 tgatgacccg cggtgtgacc gatgacagcg gtaccttgca gttacaacat atatcgccag
    10861 aacgttcata cattctgggt aaggatgctg aaggcggcgt ttttgtctcc gagaacttct
    10921 tctacgaaag cgaaatctac aacacccgct tgtatatttt taccgatcgc ccgctatatc
    10981 gcgcaggcga tcgtgtcgat gttaaagtga tgggccgcga gttccacgat ccgttgcatt
    11041 catcccccat cgtcagcgcc ccggcgaagc tttcggtgct ggacgctaac ggcagtctgt
    11101 tgcaaaccgt cgatgtcacg ctggatgcgc gcaatggcgg gcagggaagt ttccgcctgc
    11161 cagaaaatgc cgttgccgga ggttatgagt tacgtcttgc ttaccgcaat caggtctata
    11221 gcagcagttt tcgcgtggca aactacatca agccacattt cgagattggt ttagctctcg
    11281 acaaaaaaga gttcaaaact ggcgaagcgg tcagcggcaa actgcaactt ctctacccgg
    11341 atggcgagcc ggtaaaaaat gcccgcgtgc agttaagttt gcgcgctcag caattatcaa
    11401 tggtcggtaa cgatttgcgt tatgccggac gtttccccgt gtcgctggaa ggcagcgaaa
    11461 cggtgtccga cgccagcggt catgtggcgt taaatctccc cgccgccgat aaaccgagcc
    11521 gctatttgtt aaccgtctcc gccagtgacg gcgcggcgta tcgcgtcacc accaccaaag
    11581 agatcctcat tgaacgcggc ctggcgcatt actctttaag taccgccgca caatacagta
    11641 atagcggcga gtcggttgtg ttccgttatg ccgcgctgga atcttcaaaa caggttcctg
    11701 ttacgtatga atggttgcgt ctcgaagacc gcacgagcca tagcggagat ctaccgtcag
    11761 gcggcaaatc ctttaccgtc aatttcgata aacctggcaa ctacaatctg acgttacgcg
    11821 ataaagacgg cttaattctc gccgggttaa gccatgccgt cagcggtaag ggcagtatgt
    11881 cgcatactgg tacggtagat atcgtggcag ataaaacgct gtaccagcct ggcgaaaccg
    11941 cgaagatgct gattaccttc ccggagccaa ttgatgaagc attattgacg ctggaacgcg
    12001 atcgcgttga acagcagtcg ctgctatcgc atccggcaaa ctggctgacg ttacaacgtt
    12061 taaacgatac tcaatatgaa gcccgtgttc cagtgagcaa ttcctttgcg cctaacatca
    12121 ctttttcggt gctgtatacc cgtaacggtc agtacagttt tcagaacgcc gggatcaaag
    12181 ttgccgttcc ccagctggat atccgggtga aaacggacaa aacccattac cagcctggtg
    12241 aactggtcaa tgtcgaatta acctcgtcgc tgaaaggtaa acctgtttct gcgcagctaa
    12301 cggtaggcgt ggtcgatgaa atgatctacg cgctgcaacc agaaatcgcg ccgaatatcg
    12361 gcaaattttt ctatccgctg gggcgtaaca atgtgcgtac cagctccagt ctgtcgttta
    12421 tcagctacga ccaggcactc tccagcgagc cggttgcgcc tggcgcaact aaccgcagcg
    12481 agcggcgagt aaaaatgctt gaacgtccac ggcgtgaaga ggtggatacc gcggcatgga
    12541 tgccgtcact cacaaccgat aaacaaggca aagcgtattt cacgttcctg atgcctgatt
    12601 cgttaacccg ctggcgtatc accgcgcgtg ggatgaacgg cgacgggctg gtcgggcagg
    12661 ggcgtgctta tctgcgttcg gaaaaaaatc tctacatgaa gtggagtatg ccaacggtgt
    12721 atcgcgtggg cgacaaaccg tcggcaggac tgtttatctt cagtcagcag gataacgaac
    12781 cggtggcgct ggtgactaaa tttgcaggcg ctgagatgcg ccagacgctg acgctgcaca
    12841 aaggggcgaa ttatatttcg ctggcgcaga acattcagca atctggcttg ttaagcgcag
    12901 aactgcaaca aaatgggcaa gtgcaggaca gcattagcac aaaactgtct tttgtggata
    12961 acagctggcc cgttgaacag cagaaaaatg tcatgctcgg cggtggcgat aacgcgctga
    13021 tgttgcccga gcaggcgagc aatatccggc tacaaagtag tgaaacgccg caggagattt
    13081 tccgcaacaa tcttgatgcg ttagtcgatg aaccgtgggg gggggtgatc aacaccggta
    13141 gccgtctgat cccgctcagt ctcgcctggc gttcgcttgc cgatcatcaa agtgccgccg
    13201 ctaacgacat tcgtcagatg attcaggata accgtctgcg gctgatgcaa ctggcggggc
    13261 ccggagcgcg ctttacctgg tggggtgaag atggcaatgg tgacgccttc cttacggcat
    13321 gggcatggta cgccgactgg caggccagcc aggcgctcgg cgtaacgcaa caaccggaat
    13381 actggcagca tatgctcgac agttatgccg agcaggcaga taacatgccg ttattgcatc
    13441 gggcgctggt gctggcgtgg gcacaggaga tgaatctgcc gtgcaaaacg ttgttgaaag
    13501 ggttggatga agctatcgcc cggcgcggaa ctaaaactga agatttctct gaggaagaca
    13561 cccgcgatat caatgacagc ctgatcctcg atacaccgga atctccactg gcagatgcgg
    13621 tggcaaacgt cttaaccatg acgttgctga aaaaagcgca gttgaagtcc acggtgatgc
    13681 cacaggttca gcaatatgcg tgggataaag cggtaaacag caatcagccg ctggcacaca
    13741 cggttgtgct gctcaatagc gggggcgacg ctacccaggc ggctgctatt ttaagtggtt
    13801 tgaccgctga gcaatctact attgagcgcg cgctggccat gaactggctg gcgaaatata
    13861 tggcgacaat gccttcggtt gtgttgcctg cgcctgcggg cgcatgggcc aaacataagt
    13921 taactggagg gggcgaatac tggcgttggg ttggtcaggg cgtgccggac attctctctt
    13981 ttggtgacga attatcgccg caaaatgtgc aggtccgctg gcgtgaagcg gcaaaaacgg
    14041 ctcaacaaag taacattccg gtgaccgttg aacgccaatt gtatcggctt atccctggtg
    14101 aagaagagat gagctttact ctgcaaccgg tgaccagcaa tgagattgac agcgatgcgc
    14161 tgtatctcga tgaaattacg cttaccagcg agcaggatgc agttctgcgc tatggtcagg
    14221 tggaagtacc gctcccgccg ggagccgacg ttgagcgcac aacatggggc atttcagtca
    14281 ataaacccaa cgctggaaaa cagcaggggc aattgctgga aaaagcgcga aatgaaatgg
    14341 gcgaactggc ctatatggtg ccggtgaaag aactgacggg aacggtcact ttccgccatt
    14401 tgctgcgctt ctcgcaaaaa gggcaattcg ttctgcctcc tgctcgttat gtgcgttcct
    14461 atgcacctgc acagcaaagt gttgcggcag ggagcgaatg gaccgggatg caggtgaaat
    14521 aagtgaactg gcgcagaatc gtctggctat tggcgttggt tactctgcca acgcttgcgg
    14581 aggagacgcc gttacaactg gcgctgcgcg gtgcgcagca cgatcaactg tatcagctgt
    14641 cgtcatcagg cgtgaccaaa gtctcagcat tacctgacac gctcaccacg ccgttaggga
    14701 gtttatggaa actgtatgtt tacgcctggc tggaggatac ccatcaaccg gagcagccct
    14761 accagtgtcg cggaaactcc ccggaagagg tttattgttg tcaggctggg gagagcataa
    14821 cgcgcgatac cgcgctggta cgttcctgtg ggctctattt tgcgccgcag cggttgcata
    14881 ttggggcaga tgtgtggggg caatactggc aacagcgtca ggcacctgcg tggttagcgt
    14941 ctctttctac gctgaagcca gaaacctcgg taacggttaa atcgctgctt gattcattag
    15001 cgacgttacc tgcgcaaaac aaagctcagg aagtcttact ggatgtggtg ctggacgagg
    15061 cgaaaatcgg tgtcgcatct atgctcggta gtcgggtacg ggtgaaaacc tggagctggt
    15121 ttgccgatga taaacaggaa atccgtcagg gtggttttgc tggctggtta accgatggta
    15181 caccgctgtg ggtgactggc agtggcacca gcaaaacggt actgacccgt tatgcgacgg
    15241 tgcttaatcg ggttttgcca gtgccgacgc aagttgccag tgggcaatgt gtagaggtgg
    15301 agctatttgc gcgctagccg ctgaagaaaa tcacagcgga gaagagtacg acggcagtaa
    15361 aacccggcgt acttaatggg cgctatcgtg tgacgtttgc caatggtaac cacattacgt
    15421 ttgtcagcca cggtgagacg accttactga ccgaaaaggg caaactgaaa ttgcagtcgc
    15481 atctcgatcg tgaagagtac gttgccagag ttctggatcg cgaggcgaaa agtacgccac
    15541 ctgaggcagc gaaagccatg actgtggcga tacgcacttt tttacagcaa aacgccaatc
    15601 gggaaggcga ttgcctgacc atccccgaca gcagcgccac gcagcgtgtt tctgcttcgc
    15661 cagctaccac gggggcgcga acgatgaccg tctggacgca ggatctcatc tatgcgggcg
    15721 atccggttca ttatcacggc agtcgcgcta ccgaagggac gctttcctgg cggcaagcca
    15781 tggcccaggc ggggaaggga gagcgttacg accagatact cgcgtttgct tatcccgaca
    15841 acagccttag ccgctgggga gcgccacgca caacctgtca gttattaccc aaagcgaaag
    15901 cctggctggc gaaaaaaatg ccgcagtggc ggcgtatatt acaaggcgag acggggtaca
    15961 acgaaccaga cgtgtttgcg gtctgtcgtt tagtctcagg tttcccctat accgatcggc
    16021 agcagaaacg gctgtttatt cgtaacttct ttacgcttca ggatcggctg gatttaaccc
    16081 atgagtatct gcacctggcc tttgatggtt atcccaccgg gcttgatgag aactatatcg
    16141 aaacgctgac ccgacaatta ttaatggact aatgctatgc gaaagatttt tcttccgttg
    16201 ttactggtgg cgctttcgcc tgtcgctcac agtgaaggtg tgcaggaagt cgagattgac
    16261 gcaccgctct ctggctggca tccggcagag ggcgaagatg ccagtttttc acaatcgatc
    16321 aattacccgg cgtcgtccgt caacatggca gatgatcaaa atatctcagc gcagatccgc
    16381 ggcaaaataa agaattatgc tgcggcgggt aaagttcagc agggccggct ggtggtcaac
    16441 ggtgccagta tgccgcagag aattgaatcc gatggttcat ttgcacgccc ttatattttc
    16501 actgaaggca gcaacagcgt gcaggtcatc agcccggatg ggcaaagccg acaaaaaatg
    16561 cagttttact caacgccggg tacaggaacg attcgtgcac gtttacggct ggttctctcg
    16621 tgggatacgg acaataccga cctcgatctt cacgttgtca cgcccgatgg cgaacacgcc
    16681 tggtacggta acaccgtgct gaaaaacagt ggtgcattgg atatggatgt cacgacgggg
    16741 tacggacccg agattttcgc catgccagca ccagttcacg ggcgctatca ggtgtatatc
    16801 aactactatg gtgggcgcag cgaaacggaa ttaaccaccg cccaactgac gctgatcacc
    16861 gatgaaggat cggtcaatga gaaacaggaa acatttattg taccgatgcg taatgctggc
    16921 gagctgacgc tggtgaaaag ttttgactgg tgatccatcc cttcacacaa cctggctctt
    16981 tcttttttca gataagactg ctgggcgatc tgccgacgcc aggatataac cattaacaag
    17041 cgtcggcaga ttggtcattt cttatttttc gtactgagaa ctgtccattt actccggctt
    17101 tcctggttaa ggcaatggtt tctaagcgat acgcattatt gcaatggtga cagaacattg
    17161 cggtaacacg cttttaccgc taccttaacc acactccatc ggtcacctga ggcggagctt
    17221 cgcccctttg aaataccttg cttcttttcg tacaaccctg aaagcctcgc gctacatgtt
    17281 cagagcattg gcgttagtgc tctggctgtt gattgctttt tcatccgttt tttacatcgt
    17341 taatgcgtta catcagcgag aatcggaaat tcgtcaggaa tttaatctga gttccgatca
    17401 ggctcagcgc tttattcaac gcacctctga tgtgatgaaa gagctgaagt acatcgccga
    17461 aaatcgctta tcggcagaaa acggtgtgct ttccccgcgt ggacgagaaa cgcaggcgga
    17521 tgtgcctgcg tttgaaccgc tgtttgccga ctccgattgt tcagcaatga gtaacacctg
    17581 gcgaggttct ctggagtcat tggcgtggtt tatgcgctac tggcgcgata atttttctgc
    17641 ggcttacgat ctcaaccggg tatttttaat cggcagcgat aacctctgca tggccaattt
    17701 cggtctgcgt gatatgccag tggaacgcga taccgcgttg aaagctttgc atgaacgcat
    17761 caataaatat cgaaatgcac cacaagatga tagcggcagt aacctctact ggatcagcga
    17821 aggtccgcgc cctggcgtcg ggtattttta cgcgttgacg ccagtttatc tggcgaaccg
    17881 gttgcaggcg cttttgggtg tcgagcagac catccggatg gagaactttt tcttaccggg
    17941 tacgttgccg atgggggtta ccattcttga tgaaaatggt cataccctga tttcgcttac
    18001 cggaccagaa agcaaaatta aggccgatcc tcgctggatg caggaacgct cctggtttgg
    18061 ctatacggaa gggttccggg agctggtgct gaagaaaaat ctgccaccct catcgctcag
    18121 catcgtgtat tcggtgccgg ttgataaggt gctggaacgc attcgcatgt tgatccttaa
    18181 cgcaattttg ctgaatgtgc ttgccggagc tgcattgttt actctcgcgc ggatgtacga
    18241 gcgacgtatt ttcattccgg cggaaagcga cgccctgcga ctggaagaac atgagcagtt
    18301 caatcgcaag attgtcgcct ccgcgccagt gggtatctgc attttgcgta ccgctgatgg
    18361 cgtcaatatt ttaagtaacg aactggcgca tacctatctc aatatgctta cgcatgagga
    18421 ccgccaacga ctgacacaaa ttatctgtgg gcagcaggtc aattttgttg atgtcctgac
    18481 cagcaacaat accaatctgc aaatcagctt cgtccattcg cgctatcgta atgaaaacgt
    18541 ggccatttgt gtgctggtgg atgtttcttc gcgcgtgaag atggaagagt cgttgcagga
    18601 gatggcacaa gcagcggaac aggcgagcca gtcaaaatcg atgttccttg ccaccgtcag
    18661 tcatgagctg cgaacgccgc tgtatggcat tatcggtaac ctggatctgt tgcaaaccaa
    18721 agagttaccg aaaggcgtcg atcgtctggt gacggcaatg aacaactctt ccagcctgtt
    18781 gttgaaaatt atcagcgata ttctcgattt ctcgaagatt gaatcggaac agttgatgat
    18841 cgaaccgcgt gagttttcac cgcgtgaagt gatgaaccac atcaccgcca actatttacc
    18901 gctggtggta cgcaagcagt taggcttgta ctgctttatt gaaccggatg tgccagtggc
    18961 cttaaatggc gacccgatgc gtttacagca ggtcatctcc aacctgttga gtaacgccat
    19021 aaaattcacc gataccggct gtatagtttt gcatgtccgc gctgatggcg attatctctc
    19081 tatccgtgtt cgcgataccg gcgtggggat tccggcgaaa gaagtggtgc gcttgtttga
    19141 tcccttcttc caggtcggaa cgggcgtaca gcgaaacttc caggggaccg gtctgggtct
    19201 ggcgatttgt gaaaaactga tcagcatgat ggacggcgat atctcggtag attcagaacc
    19261 gggaatgggc agccagttta ccgtgcgtat tccgttgtac ggcgctcagt acccgcagaa
    19321 aaaaggcgtg gaagggttga gtggtaaacg ctgctggctg gcggtccgca atgcgtcgct
    19381 ctgtcagttc ctggaaacca gtttgcagcg cagcggcatc gtcgtaacaa catacgaagg
    19441 gcaggaaccg actcccgaag atgtgttaat cactgacgag gtagtgagta aaaaatggca
    19501 gggcagagcg gtagtgacct tctgtcgtcg acatattggt attccgctgg agaaagcgcc
    19561 aggggagtgg gtacacagtg tggctgctcc gcatgagcta ccggcattgt tggcgcgtat
    19621 ttatttgatc gagatggaga gcgacgatcc tgctaacgct ctgccgtcga cggacaaagc
    19681 ggtcagcgat aatgacgata tgatgattct ggtcgtggat gatcatccga ttaaccggcg
    19741 tttgctggca gatcagttgg gatcgttggg ctatcaatgt aaaaccgcga atgatggcgt
    19801 cgatgcgctt aatgtactta gcaagaatca tattgatatc gtgcttagcg acgtcaacat
    19861 gccaaatatg gatggttacc gcttgacaca acgcattcgt cagttgggac tgacgttgcc
    19921 ggtaatcgga gtaacggcta atgcgttggc tgaagagaag cagcggtgtc tggagtccgg
    19981 tatggacagc tgcctgtcga agccagtaac gctggatgtt ataaaacaga cgctgacggt
    20041 atatgccgag agggtcagga aatcgcggga atcgtaggac ggataaggcg tttacgccgc
    20101 atctggcatt cagtgcaaat gccagatgcg acgctgacgc gtcttatctg gcctacttta
    20161 atgtaatctg ttgaaataat gggaatcgta ggacggataa ggcgtttacg ccgcatccgg
    20221 caatcggtgc aaatgccaga taagacacta acgcgtctta tctggcctac agatgattag
    20281 tctttatctg ccggacttaa cgttactgaa gagagataat tcagcagggc gatatcgttc
    20341 tcgacaccca gcttcatcat cgcagatttc ttctggctac tgatggtttt aatactgcgg
    20401 ttcagctttt tagcgatctc ggtcaccagg aagccttccg caaacaggcg cagaacttca
    20461 ctctcttttg gcgagagacg tttgtcaccg taacctccag cactgatttt ttccaacagg
    20521 cgagaaacgc tttccggggt aaatttcttt cctttttgca gcgctgcgag ggctttcggc
    20581 agatcggtcg gtgcaccttg tttcagcacg atcccttcga tatccagatc caataccgca
    20641 ctaagaatcg ccgggttgtt gttcatggtc agaacaataa tcgacaggct tgggaaatgg
    20701 cgcttgatgt acttaattaa ggtaatgcca tcgccgtact tatcgccagg catggagaga
    20761 tcggtaatca acacatgcgc atccagtttc ggcaggttgt tgatcagtgc tgtagagtct
    20821 tcaaattcgc cgacaacatt cacccactca atttgctcaa gtgatttgcg aataccgaac
    20881 aagactatcg gatggtcatc ggcaataatt acgttcatat tgttcatgta ataggctacc
    20941 ttgctacagc aagctcttga cataactgtc aatgtcgctg atgtattttt ctattcctgg
    21001 aacatccttc tcacgaatca gatgttccag cgtttcacat aactgcttgc cgggtaccag
    21061 atttagcatg gcaaatacgc ctttaagacg atgagccgtt tgggctaacg cagcaaagtc
    21121 actggttgct gcttcagtat acagcctctt aacatcatcc ggtactgtgt ctacaaagag
    21181 cgcataatag ccgctggcat ggagttgcgc attttcatct ccgcccagag gcgattctgt
    21241 cacctcttcc tgcgccagtt gcacttcaat taattgtaag accgcttcct gcatagcgtt
    21301 gctcatattg aagttgacgc acaattgacc aggcccaatt tcccgtacgc cagactcatc
    21361 atcgcttaaa agcaagccag aggcagtaag attagacgga ttatccgtta aaaagatatc
    21421 ataatcttga ctaattaatc tttcatcggg tgtgatacag gttgcacccc aattttctaa
    21481 ctggcgagtg acaatattcc gaatttctgc cgaagtaaca tccaccatta cgcagacatc
    21541 atccagtaaa cgctcttctt cctcttcaac ttccgggtca gctgcgagca ttttgatatg
    21601 cacagagtag cgtgtaccaa gcccatcccg cgttttgatg tttaaatgac cgcccagttt
    21661 acgtgccagt tgatcgctca gccagaatgc cagcgggtcc gccttgccat agcgatcgtt
    21721 ttgggtctgg ttgataaacg ggaagtgcaa attatccatt tcatgaatgc ttacgccttc
    21781 tccggtgtcc agaatgcgga acgtcaggcg gtcttcggag gactcatcct gatcaacctc
    21841 aagggtgatt tttcccaatt gcgttgaggt cacggcatat tgcatcagta gcagcaaaat
    21901 acgtcgtaat gcatcgcgat cgccgcggcg catatcgtgt gctttcagat gattgttaat
    21961 cagcagttgc agacctttac gcttgatggc aggcaacact gaaggcacaa cttcatcaat
    22021 taaatcctgc acggagaaca gcaccgtctc acttttccag ctatcgtccg caagcatgtt
    22081 cgctaactgt atttcatcga ccaatcgcac cagcacatct gcctgattcg ccagttgttt
    22141 gctttccggg gcgttgagtt tagccgcgct ctccgccagg gactgtgcgg gttctttcag
    22201 cgcatcgcca atgtttttca taaaggtcat ccgcccctgc tggtttttct catacagacg
    22261 ctgcgcctgc ttgagtttct tgtttaccag cacttcgcga tcctgatcgc gaataatgaa
    22321 aatttgtgtg cgcggcgcaa cctggctgcg gaacatgcgg atctcataca gctcgttatt
    22381 gatcgtcgcc tgaataatcc cctgatgctg ttccgccatg gtggtgatgt tttgcagatt
    22441 caaatgcggc agcaaatgat cggcaatttt gttacttatg acagtgcggt tcgattcctg
    22501 atcgtgaacc agcaggccga gcggcagcag tgagactatc tcttcattga ttgcccgtaa
    22561 aatgcgcaat tcgttattga ccgccgtgtt gggtagactt tctgtactgc gactggagaa
    22621 atggcggaat gtggtatagc caaataacgc cagcgccagc aaaccgatgt tcagcagcag
    22681 tggcagcaga atgttttgca acgtatccag caataaggtg ccataaggaa cctgccagac
    22741 caggcgcata tcggtagagt tgagtgccga ggagatttca atcttcgtac tgttaaagtg
    22801 gatactgacg ctatccgttc cttctttctc attatcattg tttcccgttg ccgtcgcgtc
    22861 tggctcaagg cggaaactgt ccagcggcat acccggtggg atcaaatcat taatcggcag
    22921 atcaaaagcc acgaccgttg ccagatgtcc cggctggttg aacgtggtac gcaaggtaaa
    22981 gtaatgaccg ttctgccagg ccaggcggcg catgttagaa aaactttcgc gttcatcgag
    23041 ggcgttggcc tgttgcaaca tctctgcacg acgtgaatca acaatgtcac tgacggtcga
    23101 ttctttaaat ccggaggtga gatctttgag gggcagggtt gagatcagca ccagactgtt
    23161 atcctgacca ttcaggtaat acattgacca cggtacattt tctgcgcccc acaatgtgtc
    23221 cagataggtg gacatccgtt gagtcatctc aagcgttgag ttgtcgtgag agccaaagat
    23281 taacgcttcc gttttgcggc gcggtttttc cagatagtag acatcctgtt tcaggcgcgt
    23341 ctcttgtaaa ccttcgccgg aggaggggga ggtcgtcgcg gcaatgttgt cgtagatctg
    23401 ccaggtcacg taacgccagg tatcgacgcg cttttggata gcgtgggtaa tgtcgacaat
    23461 ttggtaactt ttatctttca gccaggcgtt aacggcgctt tgtaccatta cacccatcgt
    23521 caccagtaac acaatgatca acagtaaaaa gaagcgggta atgctccccg gtaggagtga
    23581 aaagcgggtc gtggccgttg tctctttctg acgcattcgt gtttatgacc tgttaaaact
    23641 tcgcgaagtg atagggcagt ataaagggta cagtgtgaaa ttccagactc ttacctgatt
    23701 gatgattctc tattctcccg tgatatttat catcctgcat tgggggaaat agatgtacaa
    23761 gcgccatttt gctgtacaaa taacctacaa aaagcccaac gaaaataatt gcaaataaac
    23821 aaagattgct ggaaattatg ctgatgttaa ttatttgtga aatagttaac aagcgttata
    23881 gtttttctgt ggtagcacag aataatgaaa agtgtgtaaa gaagggtaaa aaaaaccgga
    23941 tgcgaggcat ccggttgaaa taggggtaaa cagacattca gaaatgaatg acggtaataa
    24001 ataaagttaa tgatgatagc ggtagttatt ctagttgcga gtgaaggttt tgttttgaca
    24061 ttcagtgctg tcaaatactt aagaataagt tattgatttt aaccttgaat tattattgct
    24121 tgatgttagg tgcttatttc gccattccgc aataatctta aaaagttccc tcgaatttac
    24181 attttgaaac atctatagcg ataaatgaaa catcttaaaa gttttagtat catattcgtg
    24241 ttggattatt ctgcattttt ggggagaatg gacttgccga ctgattaatg agggttaatc
    24301 agtatgcagt ggcataaaaa agcaaataaa ggcatataac agagggttaa taacatgaaa
    24361 gttaaagtac tgtccctcct ggtcccagct ctgctggtag caggcgcagc aaacgctgct
    24421 gaagtttaca acaaagacgg caacaaatta gatctgtacg gtaaagtaga cggcctgcac
    24481 tatttctctg acgacaagtc tgtagatggc gaccagacct acatgcgtct tggcttcaaa
    24541 ggtgaaactc aggttactga ccagctgacc ggttacggcc agtgggaata tcagatccag
    24601 ggcaacagcg ctgaaaacga aaacaactcc tggacccgtg tggcattcgc aggtctgaaa
    24661 ttccaggatg taggttcttt cgactacggt cgtaactacg gcgtagttta cgacgtaact
    24721 tcctggaccg acgttctgcc agaattcggt ggtgacacct acggttctga caacttcatg
    24781 cagcagcgtg gtaacggttt cgcgacctac cgtaacactg acttcttcgg tctggttgac
    24841 ggcctgaact ttgctgttca gtaccagggc aaaaacggta gcgtaagcgg cgaaggcatg
    24901 actaacaacg gtcgtgaagc actgcgtcag aacggcgacg gcgttggcgg atctatcact
    24961 tatgattacg aaggcttcgg tatcggtgct gcagtttcca gctccaaacg tactgatgat
    25021 cagaacagcc cgctgtacat cggtaacggc gaccgtgctg aaacctacac tggtggtctg
    25081 aaatacgacg ctaacaacat ctatctggct gctcagtaca cccagaccta caacgcaact
    25141 cgcgtaggtt ccctgggttg ggcgaacaaa gcacagaact tcgaagctgt tgctcagtac
    25201 cagttcgact tcggtctgcg tccgtccctg gcttacctgc agtctaaagg taaaaacctg
    25261 ggtgtcatca atggtcgtaa ctacgacgac gaagatatcc tgaaatatgt tgatgttggc
    25321 gcgacctact acttcaacaa aaacatgtcc acctatgttg actacaaaat caacctgctg
    25381 gacgacaacc agttcactcg tgacgctggc atcaacactg ataacatcgt agctctgggt
    25441 ctggtttacc agttctaatc tcgattgata tcgaacaaag ggcctgcggg cccttttttc
    25501 attgttttca gcgtacaaac tcagtttttt ggtgtactct tgcgaccgtt cgcatgagga
    25561 taatcacgta tggaaataag ctttacccgc gtggcactgc tggctgccgc gctcttcttt
    25621 gttggttgcg atcaaaaacc acaacccgcc aaaacccacg ctactgaagt taccgttctt
    25681 gaaggcaaaa ctatgggtac cttctggcgt gccagcatcc cgggcattga cgccaaacgc
    25741 agcgccgaac ttaaagaaaa gattcagacc cagctggacg ccgacgatca gctgctttcg
    25801 acctataaaa aagattccgc gctgatgcgc tttaacgact cgcaaagttt atcgccgtgg
    25861 ccggtaagtg aagcgatggc cgatatcgtc accacctcgc tacgcattgg cgcgaagacc
    25921 gatggcgcga tggatataac cgtcgggccg ctggtgaatc tgtggggttt cggtccggaa
    25981 caacagccgg ttcaaattcc gagccaggaa cagatcgacg cgatgaaagc caaaactggt
    26041 ttacagcacc ttacggtcat taatcagtcg catcagcaat atctgcaaaa agacctgccg
    26101 gatttatatg tcgatctctc caccgtcggc gaaggttatg cggcggatca cctggcacgc
    26161 ttgatggagc aggaagggat ttcccgctat ctggtgtcgg tgggcggcgc gctgaacagc
    26221 cgtggtatga acggtgaagg cctgccgtgg cgggtagcga ttcaaaaacc aaccgataaa
    26281 gaaaacgcgg ttcaggccgt ggtggatatc aacggtcatg ggattagcac ctctggcagc
    26341 taccgtaact attacgaact ggacggcaaa cgtctttccc atgttatcga tccgcaaacc
    26401 gggcgtccca tcgaacacaa tctggtatcc gtgacggtga ttgcaccgac ggcgctggaa
    26461 gccgacgcct gggatacagg tttgatggta ctcgggccgg agaaagccaa agaagttgtt
    26521 cgccgggaag ggctggcggt ttatatgatc accaaagaag gcgatagctt taaaacctgg
    26581 atgtcaccac agtttaaaag cttccttgtc agcgaaaaaa attaaagcgc aagattgttg
    26641 gtttttgcgt gatggtggcc gggcagacta aaggctatcc ttaaccaggg agctgattat
    26701 gaaaaacgcc acatgcttaa ctgacgatca acgctggcaa tctgtcttag cccgcgaccc
    26761 gaatgccgac ggcgaattcg ttttcgccgt gcgcaccaca ggcatctttt gccgtccgtc
    26821 ttgccgcgcc agacatgctt tgcgggaaaa cgtctccttc tacgcaaatg ccagcgaggc
    26881 actcgccgcc ggatttcgcc cctgcaaacg ctgccaacct gataaagcca atgcccagca
    26941 acatcggttg gataaaatca cccacgcgtg tcgactgctg gaacaggaaa cgcctgtaac
    27001 gttggaagcc ttagccgacc aggtggcgat gagtccattt catctacatc ggttgtttaa
    27061 agcgactacc ggaatgacgc ctaaagcctg gcaacaggcc tggcgcgctc gccgtttgcg
    27121 cgaatcgctg gcgaaagggg agagcgtgac gacgtctatt cttaacgccg gattccccga
    27181 cagcagcagt tactaccaca aagccgatga aacgctgggc atgacggcta aacaattccg
    27241 tcacggtggc gaaaatctgg cggtgcgtta cgcgctggct gattgtgagc tgggtcgttg
    27301 cctggtggca gaaagcgagc gggggatttg cgcgatattg ctgggcgatg atgacgcgac
    27361 attaatcagc gagttgcagc agatgtttcc cgctgccgac aacgcgcctg ccgatctgac
    27421 gtttcagcaa catgtgcgtg aagtgatcgc cagcctcaat caacgcgata ctccgctgac
    27481 gttaccgctg gatattcgcg gcactgcttt tcagcaacaa gtctggcagg cactgcgcac
    27541 gataccttgc ggtgaaaccg tcagttatca gcaactggct aacgccatcg gcaaaccgaa
    27601 agcggtacgg gccgttgcca gcgcctgtgc cgccaacaag ctggctatcg taattccctg
    27661 tcatcgagtg gtccgtggtg atggcacact ttccggttac cgctggggcg tgtcgcgtaa
    27721 agcgcaactg ctgcgccgcg aagctgaaaa tgaggagagg taatgttgga tctgtttgcc
    27781 gatgctgaac cgtggcaaga gccactggcg gctggtgcgg taattttacg gcgttttgct
    27841 tttaacgctg cagagcaact aatccgcgat attaatgacg ttgccagcca gtcgccgttt
    27901 cgccagatgg tcacccccgg gggatatacc atgtcggtgg cgatgaccaa ctgtgggcat
    27961 ctgggctgga cgacccatcg gcaaggttat ctctattcgc ccattgatcc gcaaacaaat
    28021 aaaccgtggc ccgccatgcc gcagagtttt cataatttat gtcaacgtgc ggctacggcg
    28081 gcgggctatc cagatttcca gccagatgct tgtcttatca accgctacgc tcctggcgcg
    28141 aaactgtcgc tgcatcagga taaagacgaa ccggatctgc gcgcgccaat tgtttctgtt
    28201 tctctgggct tacccgcgat ttttcaattt ggtggcctga aacgtaatga tccgctcaaa
    28261 cgtttgttgt tggaacatgg cgatgtggtg gtatggggcg gtgaatcgcg gctgttttat
    28321 cacggtattc aaccgttgaa agcggggttt catccactca ccaccgactg ccgctacaac
    28381 ctgacattcc gtcaggcagg taaaaaagaa taaaaataag aattattatt gctgtgcgcg
    28441 cgaagatgtt taaactgcgg gctgtaattc attgtccggg ttttctgcat ggaacttctt
    28501 gtacttgtct ggcggcagta tcgctggcca tttatcagtg tgatggcgct aagcctcgcc
    28561 agtgcggctt taggcattgg cttaattgct tttatcaatc agcgccttat cgaaacggcg
    28621 gataccagtc tgctggtgtt gccggagttt ctgggattat tgctgctgtt gatggcagtc
    28681 actctcggat cgcaactggc gctcaccact ttggggcatc acttcgttta ccgactgcgt
    28741 agtgaattta tcaagcggat tctggatact cacgtcgagc gcattgaaca actcggtagc
    28801 gcctcgttgc tggcgggatt aaccagcgat gtgcgcaata tcaccattgc ttttgtgcgt
    28861 ctgccggaac tggtgcaggg gatcattctc actatcggtt cagcggcgta tctgtggatg
    28921 ctgtcgggca aaatgttgct ggtaacggct atctggatgg cagtcaccat ctggggcggt
    28981 tttgtgctgg tggcgcgggt gtacaaacat atggcgaccc tgcgtgaaac cgaagacaag
    29041 ctgtacacgg attttcaaac tgtacttgaa gggcgcaaag agctgactct gaaccgggaa
    29101 cgcgccgagt atgtgtttaa caacctctac attcctgatg cgcaagagta tcgccatcat
    29161 attattcgcg cagacacctt ccatcttagt gccgtgaact ggtcaaacat catgatgctg
    29221 ggcgctatcg gcctggtgtt ctggatggcg aacagtctcg gttgggctga taccaacgtt
    29281 gccgcgacct attcgttgac gcttttattc ctgcgtacgc cgctgctttc ggcggttggc
    29341 gcattgccga cgctgctgac ggcgcaggtg gcgtttaaca agctgaacaa attcgcgctc
    29401 gcgcctttca aagcagagtt tccgcgcccg caggcgtttc ccagctggca aacgctggag
    29461 ctgcgtaacg tgacgtttgc ttatcaggat aacgcgtttt ccgttggtcc gattaatctc
    29521 accatcaaac gtggcgagct gctgtttctg attggcggca acggtagcgg aaaatcgacg
    29581 ctggcgatgt tgttgacggg cttgtatcag ccacaaagcg gcgaaatctt gctggatggc
    29641 aaacctgtca gcggcgaaca accggaagat tatcgcaaac tgttttcggc agtgtttacc
    29701 gatgtctggc tgtttgatca actgctgggg ccggagggta aacccgctaa cccgcaactg
    29761 gttgagaagt ggctggcgca gctgaaaatg gctcataagc ttgagttaag aaacgggcgt
    29821 attgttaacc tgaagttatc aaaagggcag aaaaaacgcg tggcgctgtt gctggcgctg
    29881 gcagaagaac gcgatattat cctgctggat gaatgggcgg cggatcagga tccacacttc
    29941 cgtcgtgagt tttatcaggt gttgctgccg ctgatgcagg agatgggtaa aactattttc
    30001 gccatcagtc atgatgatca ttactttatc cacgccgacc gcctgctgga aatgcgcaac
    30061 gggcaactta gcgagctgac gggcgaagag cgcgatgccg cttcgcgtga tgccgttgcc
    30121 cggacggcat aatattacga cgctgacctg gcggactgct gccgccaggt caatatgcta
    30181 aaaatccctt catttctaat aaccctataa ttaatcaacg aaattataat gtttctaaaa
    30241 ttagaatata atttataaac attatttaaa tgttgttact taagtgttac cgttgatgcc
    30301 gcgcaaatct cacctgcaat aaagcgacta aaagtaaggc attaacaaga tgaaaaaagt
    30361 gactgccatg ctcttctcga tggccgtggg gcttaatgcc gtttcgatgg cggcaaaagc
    30421 gaaagcgtcc gaggagcagg aaactgatgt actgttgatt ggcggcggca ttatgagcgc
    30481 cacgttgggg acctatttac gcgagctgga gcctgaatgg tcgatgacca tggtggagcg
    30541 cctggagggt gtcgcgcagg agagttcgaa cggctggaat aacgccggaa ccgggcattc
    30601 tgcactgatg gaactgaact acaccccgca aaacgccgat ggcagcatca gtattgaaaa
    30661 agcagtcgcc attaacgaag catttcagat ttcccgccag ttctgggcgc accaggtcga
    30721 gcgcggcgtg ctgcgtactc cgcgttcatt tatcaatacc gttccgcata tgagctttgt
    30781 ctggggcgag gataacgtca atttcctgcg cgcccgttac gccgcgttgc aacaaagctc
    30841 gctgtttcgc ggtatgcgtt actctgaaga tcacgcgcag atcaaagagt gggcaccgtt
    30901 agtgatggaa gggcgcgatc cgcaacagaa agtggcagcc acgcgtacgg aaattggtac
    30961 cgatgtgaac tacggcgaga tcacccgcca gttaattgct tccttgcaga agaaatctaa
    31021 cttctcgctg caactcagca gcgaagtccg cgccctaaag cgtaatgacg ataacacctg
    31081 gaccgttacc gttgccgatc tgaaaaatgg cactgcacag aacatccgtg cgaaatttgt
    31141 ctttatcggc gcgggcggtg cggcactgaa gctgttacag gaatcgggga ttccggaagc
    31201 gaaagactac gccggtttcc cggtgggcgg acagttcctt gtttcggaaa acccggacgt
    31261 ggttaatcac catctggcga aggtttacgg taaagcatcc gttggcgcac caccgatgtc
    31321 ggttccgcat atcgataccc gcgttctgga cggtaaacgc gtagtgctgt ttgggccatt
    31381 tgccaccttc tcaaccaaat tcctcaaaaa cggttcattg tgggatctga tgagttccac
    31441 caccacctct aacgtgatgc cgatgatgca cgtcgggctg gataatttcg atctggtgaa
    31501 atatctggtg agtcaggtga tgttgagtga agaggatcgt tttgaagcgt tgaaagagta
    31561 ctatccgcaa gcgaaaaaag aggactggcg tttgtggcaa gcggggcagc gtgtgcagat
    31621 tatcaagcgt gatgccgata aaggtggtgt actgcgtctg ggtactgaag tggtcagtga
    31681 ccagcaagga accattgccg cgctcctggg ggcatcgcca ggggcgtcaa ccgccgcgcc
    31741 gattatgttg aatctgctgg aaaaagtatt tggcgatcgc gtttccagcc cgcaatggca
    31801 ggctacgttg aaagcgatcg ttccatctta tggacgcaag ctgaacggtg atgtagcggc
    31861 aacagaacgc gagttgcagt acaccagcga agtgctgggg ctgaaatacg acaaaccgca
    31921 agcagcagat agtacgccga aaccgcagtt gaaaccgcaa cccgttcaaa aagaagtggc
    31981 ggatattgcg ttgtaatgat gtgccacatc cggcatggta tgccggatgt ggcgtatgct
    32041 gataagacgc gccagcgtcg catcaggcaa ccggctcggg cgttagatgt cggatgcggc
    32101 gtgaccgctt tatccaacct acggggtgcc gcacttcacg gcagtttagc gaactaccgc
    32161 gttgtcaatt ttctcttccg ccttccagac gcggtacttc acatctacat tgtctggcgt
    32221 atacaccacg atcggcagct tgctgttgta acgcagcatt ccagtatcgc ccagatacgc
    32281 ggtgacaaat ttcttctctt tcttgccatc cgggcaggcc atcatcgtgg aaaccggcga
    32341 gctaacttta tcaaagacat aataatcata gccccagcct tccagcgttt tgctttccag
    32401 cttcccgccg agacgatgca aattgcaatc gacttccagc gtctgaccga ttaacagttc
    32461 tactttcagg gtagattcat cttcttgcgg ggttaactga atcacctgac gcttcatgcc
    32521 tttttcagct tgtggataag gcgcgatttt ttccagtggc tggacgctct ctgccgccca
    32581 ggcggaagtg gtggcgaaag cggcaaacaa tactgcaggt agaatggtct tcattttttg
    32641 ctttccttct tcatttatag gtttgcaccg atgatgtcgc tgaaggttaa catattcatc
    32701 agatttatta
//
LOCUS       DAZFAB010000045        32334 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZFAB010000045 DAZFAB010000000
VERSION     DAZFAB010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32334
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.045"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(85..1131)
                     /gene="aroH"
                     /locus_tag="ACHJWA_002405"
     CDS             complement(85..1131)
                     /gene="aroH"
                     /locus_tag="ACHJWA_002405"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFO4308990.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRINHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(1288..2121)
                     /gene="ppsR"
                     /locus_tag="ACHJWA_002406"
     CDS             complement(1288..2121)
                     /gene="ppsR"
                     /locus_tag="ACHJWA_002406"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFO4308991.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            2454..4832
                     /gene="ppsA"
                     /locus_tag="ACHJWA_002407"
     CDS             2454..4832
                     /gene="ppsA"
                     /locus_tag="ACHJWA_002407"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFO4308992.1"
                     /translation="MSNNGSSPLVLWYNQLGMKDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(4889..6535)
                     /gene="fadK"
                     /locus_tag="ACHJWA_002408"
     CDS             complement(4889..6535)
                     /gene="fadK"
                     /locus_tag="ACHJWA_002408"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFO4308993.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFTPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCSCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(6592..6885)
                     /locus_tag="ACHJWA_002409"
     CDS             complement(6592..6885)
                     /locus_tag="ACHJWA_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO4308994.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(6882..8171)
                     /locus_tag="ACHJWA_002410"
     CDS             complement(6882..8171)
                     /locus_tag="ACHJWA_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO4308995.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDILVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAASAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLADIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(8227..9165)
                     /locus_tag="ACHJWA_002411"
     CDS             complement(8227..9165)
                     /locus_tag="ACHJWA_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFO4308996.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSSLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSELYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(9185..9949)
                     /locus_tag="ACHJWA_002412"
     CDS             complement(9185..9949)
                     /locus_tag="ACHJWA_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFO4308997.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYMVQDAQLEHALPLDTAKALA
                     AAVEKIGFDLLIFGKGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIGWSQSAPLAEL
                     TGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            10265..11176
                     /locus_tag="ACHJWA_002413"
     CDS             10265..11176
                     /locus_tag="ACHJWA_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4308998.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENHLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKKDVLIKDILFYLNNNYREKITLEQLSKKFRASVSYICHEFT
                     KEYRISPINYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPS
                     DYRRQFKNCFAEQEILSEFPQPVSLAG"
     gene            complement(11219..12370)
                     /locus_tag="ACHJWA_002414"
     CDS             complement(11219..12370)
                     /locus_tag="ACHJWA_002414"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFO4308999.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(12384..13979)
                     /locus_tag="ACHJWA_002415"
     CDS             complement(12384..13979)
                     /locus_tag="ACHJWA_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HFO4309000.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SDLAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQSQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGSNVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEIADGKLNIVQEGRVKKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            complement(14124..14882)
                     /gene="aroD"
                     /locus_tag="ACHJWA_002416"
     CDS             complement(14124..14882)
                     /gene="aroD"
                     /locus_tag="ACHJWA_002416"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFO4309001.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLALLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(14913..15779)
                     /gene="ydiB"
                     /locus_tag="ACHJWA_002417"
     CDS             complement(14913..15779)
                     /gene="ydiB"
                     /locus_tag="ACHJWA_002417"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFO4309002.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDKF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENKSLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(15791..17056)
                     /locus_tag="ACHJWA_002418"
     CDS             complement(15791..17056)
                     /locus_tag="ACHJWA_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4309003.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(17283..18497)
                     /locus_tag="ACHJWA_002419"
     CDS             complement(17283..18497)
                     /locus_tag="ACHJWA_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4309004.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQSNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFSFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(18597..18953)
                     /locus_tag="ACHJWA_002420"
     CDS             complement(18597..18953)
                     /locus_tag="ACHJWA_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFO4309005.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGRCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(19089..19196)
                     /gene="rprA"
                     /locus_tag="ACHJWA_002421"
     ncRNA           complement(19089..19196)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHJWA_002421"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(19382..20494)
                     /gene="ydiK"
                     /locus_tag="ACHJWA_002422"
     CDS             complement(19382..20494)
                     /gene="ydiK"
                     /locus_tag="ACHJWA_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFO4309006.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIQKPNK"
     gene            20883..23939
                     /locus_tag="ACHJWA_002423"
     CDS             20883..23939
                     /locus_tag="ACHJWA_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFO4309007.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGLRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCARLDELIASHQAGVIGWQMCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            23936..24346
                     /gene="menI"
                     /locus_tag="ACHJWA_002424"
     CDS             23936..24346
                     /gene="menI"
                     /locus_tag="ACHJWA_002424"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFO4309008.1"
                     /translation="MIWKRKIPLEALNAMGEGNMVGLLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEISDEKGRLCCSSRLTTAIL"
     gene            24446..24634
                     /locus_tag="ACHJWA_002425"
     CDS             24446..24634
                     /locus_tag="ACHJWA_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFO4309009.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            25183..25551
                     /gene="sufA"
                     /locus_tag="ACHJWA_002426"
     CDS             25183..25551
                     /gene="sufA"
                     /locus_tag="ACHJWA_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFO4309010.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAVHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            25560..27047
                     /gene="sufB"
                     /locus_tag="ACHJWA_002427"
     CDS             25560..27047
                     /gene="sufB"
                     /locus_tag="ACHJWA_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFO4309011.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            27057..27803
                     /gene="sufC"
                     /locus_tag="ACHJWA_002428"
     CDS             27057..27803
                     /gene="sufC"
                     /locus_tag="ACHJWA_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFO4309012.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            27778..29049
                     /gene="sufD"
                     /locus_tag="ACHJWA_002429"
     CDS             27778..29049
                     /gene="sufD"
                     /locus_tag="ACHJWA_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFO4309013.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVPALSDATE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLELAEGAEATVIEHFVSLNDARHFTGARFSINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENCTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLTRIGQRLPGGAR"
     gene            29046..30266
                     /gene="sufS"
                     /locus_tag="ACHJWA_002430"
     CDS             29046..30266
                     /gene="sufS"
                     /locus_tag="ACHJWA_002430"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFO4309014.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSAQGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            30279..30695
                     /gene="sufE"
                     /locus_tag="ACHJWA_002431"
     CDS             30279..30695
                     /gene="sufE"
                     /locus_tag="ACHJWA_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFO4309015.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDVVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            30844..31848
                     /gene="ldtE"
                     /locus_tag="ACHJWA_002432"
     CDS             30844..31848
                     /gene="ldtE"
                     /locus_tag="ACHJWA_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFO4309016.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVAVSSGATPTASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(31911..32147)
                     /gene="lpp"
                     /locus_tag="ACHJWA_002433"
     CDS             complement(31911..32147)
                     /gene="lpp"
                     /locus_tag="ACHJWA_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFO4309017.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         8223 a         7971 c         8223 g         7917 t
ORIGIN      
        1 atggctgacc agagcaagca gtaatgtgtt gagaagacaa caagttgcgc agaaatgccc
       61 atcccatcag gaatgggcac gcattcagaa gcgggtatct accgcagagg ctagtttttc
      121 gaccaggcgt tcggtatctt cccagcccag acacgggtcg gtaatggatt gaccgtaagt
      181 gagcggctga ccgccgacga ttttttgcgt tccttcgcgc aggaaacttt ccgccataat
      241 tccagcaatc gccgtagagc cattgcggat ttgctgacaa atatcctcac aaacttctaa
      301 ctggcgacgg tgctgcttct ggcagttacc gtggctgaaa tccaccacca gatgttcagg
      361 taaatcaaac tcgtgcagcg tatcgcaggc tgcggcgata tcatcggcat gataattcgg
      421 ttttttgccg ccacgcataa taatgtggcc atacgggttg ccgctggtct gatagatggt
      481 catctgacca tttttgtctg gcgagaggaa catatggctg gcgcgggctg cgcggatagc
      541 atccacagca atccgcgtat tgccatcggt accattttta aaacctaccg gacaggagag
      601 tgccgaagcc atttcacggt ggatctgact ttcggtagta cgtgcgccaa tcgcgcccca
      661 actgattaaa tcagcaataa actgaccggt caccatatcg aggaactcag tcgcggttgg
      721 gacgcccagc tcatttacct gtaaaagtaa tttgcgcgcc agctccagac cgtgatttat
      781 ccgatagctg ccgtttaaat ctggatcgga gattagtcct ttccagccga caacagttcg
      841 tggtttttca aaataggtgc gcattacgat ttccagccgt gactggtact ggttgcgcag
      901 cgactgcaga cgggtggcgt actccattgc agcggtgaga tcgtggatcg agcaggggcc
      961 aataatgacc aacagtcgct tatcttcacc attgagtatt ttttcaattc tgcggcggga
     1021 gtcggtgaca tgggtggcga cgccaggcgt tacgggatac cgtagcgcga gttcggcggg
     1081 cgttaccagg ctctcaatac gcgcagtacg gagttcgtca gttctgttca ttacaagtct
     1141 cagaaatttg ttgcttctac ggcaggactg ccgggaagtg atgcgcatca cgataaacca
     1201 atcgctgctg atttcaagtg cgaggtgtgt cacacctcgc cgggtggtta gcatgataac
     1261 aaaaaaataa gctaatgcac tagttctcta gtacattcgg cgactaaggc ccatgatatc
     1321 gaggatcttg gtggcaatct cttctaccga ataattggta ctgttaatcc acgggatctg
     1381 atttttacgg tacaaggctt ccacttccgc gacttccatc ctgcactgac gaagcgaggc
     1441 atagcgactg ttctcccgac gttcctcgcg aatcgccgcc agacgttccg ggtcgatagt
     1501 caggccgaac aatttatgct gaagcggttt gagcgacgcg ggtagcacca gattatccat
     1561 atcgtcggca ataaaggggt agtttgccgc gcggatacca aattgcattg ccagatacag
     1621 actggtgggg gttttaccac agcgagaaac accgagcagg atcacctgag cctgatccag
     1681 attgcgcaac gaaatgccgt catcgtgggc gagggtgtaa tcaatcgccg caatgcgcgc
     1741 atcatattta ttgagattat tagggttaag gccatgggta cgatgagcaa tcggcgttgg
     1801 atccagtttc atctcttgtt gtagcggggc aaccagcgcc tgaacgatat cctggcaaaa
     1861 accttcactt tgcaagatga tggcgcgaat ttccggcaac acgatggagt agaagaccag
     1921 cggacgcacg cctgtctggt gataaattgc gtcaatctga tccttcactg cccgtgcacg
     1981 gctctcattt tcgacaaacg gcagcgtgat gctgctgata gtgacgggaa attgtgacat
     2041 tactgcgtgt cctaatacct ccgcagttat tgccgtacca tcagaaatat aaaaaacgtg
     2101 gcgatcaaca gcattatcca ttttgttctt cccgtgatgc agacataatt ctatgaaagc
     2161 ataaattaaa aacgcacaga agcgtagaac gttatgtctg gtttataaaa tgaaccttca
     2221 attttatttt ttatgaaaac agcatttcat ttttatggtt tcgtttatac cgatggttta
     2281 tgtggaaatt gtcgaaaaga gcagatttgc gcaacgctgg gatcagtctt aacaagtaaa
     2341 aaaatatatt tgcttgaacg attcaccgtt tttttcatcc ggttaaatat gcaaagataa
     2401 atgcgcagaa atgtgtttct caaaccgttc atttatcaca aaaggattgt tcgatgtcca
     2461 acaatggctc gtcaccgctg gtgctttggt ataaccaact cggcatgaag gatgtagaca
     2521 gggttggggg caaaaatgcc tccctgggtg aaatgattac taacctttcc ggaatgggtg
     2581 tttccgttcc gaatggtttc gccacaaccg ccgacgcgtt taaccagttt ctggaccaaa
     2641 gcggcgtaaa ccagcgcatt tatgaactgc tggataaaac ggatattgac gatgttactc
     2701 agcttgcgaa agcgggcgcg caaatccgcc agtggattat cgacactccc ttccagcctg
     2761 agctggaaaa cgccatccgc gaagcctatg cacagctttc cgccgatgac gaaaacgcct
     2821 cttttgcggt gcgctcctcc gccaccgcag aagatatgcc ggacgcttct tttgccggtc
     2881 agcaggaaac cttcctcaac gttcagggtt ttgacgccgt tctcgtggca gtgaaacatg
     2941 tatttgcttc tctgtttaac gatcgcgcca tctcttatcg tgtgcaccag ggttacgatc
     3001 accgtggtgt ggcgctctcc gccggtgttc aacggatggt gcgctctgac ctcgcatctt
     3061 ctggcgtgat gttctccatt gataccgaat ccggctttga ccaggtggtg tttatcactt
     3121 ccgcatgggg ccttggtgag atggtcgtgc agggtgcggt taacccggat gagttttacg
     3181 tgcataaacc gacactggcg gcgaatcgcc cggctatcgt gcgccgcacc atggggtcga
     3241 aaaaaatccg catggtttac gcgccgaccc aggagcatgg caagcaggtt aaaatcgaag
     3301 acgtaccgca ggaacagcgt gacatcttct cgctgaccaa cgaagaagtg caggaactgg
     3361 caaaacaggc cgtacaaatt gagaaacact acggtcgccc gatggatatt gagtgggcga
     3421 aagatggcca cactggtaaa ctgttcattg tgcaggcgcg tccggaaacc gtgcgctcac
     3481 gcggtcaggt catggagcgt tatacgctgc attcacaggg taagattatc gccgaaggcc
     3541 gtgctatcgg tcatcgcatc ggtgcgggtc cggtgaaagt catccatgat atcagcgaaa
     3601 tgaaccgcat cgaacctggt gacgtgctgg tcactgacat gaccgacccg gactgggaac
     3661 cgatcatgaa gaaagcatct gccatcgtca ccaaccgtgg cggtcgtacc tgtcacgcgg
     3721 cgatcatcgc tcgtgaactg ggcattccgg cggtagtggg ctgtggtgat gcaacagaac
     3781 ggatgaaaga cggtgagaac gtcactgttt cttgtgccga aggtgatacc ggttacgtct
     3841 atgcggagtt gctggaattt agcgtgaaaa gctccagcgt agaaacgatg ccggatctgc
     3901 cgttgaaagt gatgatgaac gtcggtaacc cggaccgtgc tttcgacttc gcctgcctgc
     3961 cgaacgaagg cgtgggcctt gcacgtctgg aatttatcat caaccgtatg attggcgtcc
     4021 acccacgcgc actgcttgag tttgacgatc aggaaccgca gttgcaaaac gaaatccgcg
     4081 agatgatgaa aggttttgat tctccgcgtg aattttacgt tggtcgtctg actgaaggga
     4141 tcgcgacgct gggtgccgcg ttttatccga agcgcgtcat tgtccgtctc tctgatttta
     4201 aatcgaacga atatgccaac ctggtcggtg gtgagcgtta tgagccagat gaagagaacc
     4261 cgatgctcgg cttccgtggc gcgggacgct atgtttccga cagcttccgc gactgtttcg
     4321 cgctggagtg tgaagcagtg aaacgtgtgc gcaacgacat ggggctgacc aacgttgaga
     4381 tcatgatccc gttcgtgcgt accgtagatc aggcgaaagc ggtggttgag gaactggcgc
     4441 gtcaggggct gaagcgtggt gagaacgggc tgaaaatcat catgatgtgt gaaatcccgt
     4501 ccaacgcctt gctggccgag cagttcctcg aatatttcga cggcttctca attggctcaa
     4561 acgacatgac gcagctggcg ctcggtctgg atcgtgactc cggcgtggtg tctgaactgt
     4621 tcgatgagcg caacgatgcg gtgaaagcac tgctgtcgat ggcgattcgt gccgcgaaga
     4681 aacagggcaa atatgtcggg atttgcggtc agggtccgtc cgaccacgaa gactttgccg
     4741 catggttgat ggaagagggg atcgatagcc tgtctctgaa cccggacacc gtggtgcaaa
     4801 cctggttaag cctggctgaa ctgaagaaat aaaataaatc cccggcggcg ttttgtcgcc
     4861 gggtttatgt gatccccgaa gatgaaactt attcaatctc ttcacagaca tcctgcgtta
     4921 atcgccgcat aatatctttc cgtaacaaaa acttttgtat tttacctgag gcagtgcgcg
     4981 gtagtttttc gattaccacg atatgttcag gatatttata ttttgcgacc cgtttacggc
     5041 taaaaaaagc caccacctct tccagcgata atgaatgatg cggcgctttc agcacgacat
     5101 aagcgcatga tcgttcacct aaacgttcat cgggcattgc aaccacacag gcatcgtgaa
     5161 ttttaggatg ctgcaataaa atatcttcca cttcacggct gctaatattt tcgccgccgc
     5221 ggacaataat atcttttttg cgtccggtaa tttttatata gccagcctca tccatacggc
     5281 agagatcgcc gctgtaatac cagccttctt catccagggc acgggcggtt aattcaggtt
     5341 catcaaaata tcccataaac acattggggc cacgcgaggc ttcttcacct tcgcaacctg
     5401 gcggtaaggt cttgcgtgcg tcatcgacca ctttaatctc tacacctgcg gcagcgtaac
     5461 catcggtgtg cataaagcgc gacaaaggat catcgagatt caccaccgca tgcggcgaac
     5521 tttctgtgga accataaaca cttaataatt taatgccgcg ctgctggcat tcacgcgcca
     5581 cttttttggg gattgtggta ccgccgcaaa gaaagaagcg cagcgctgaa aggtctgcgg
     5641 gttgtttctc tagtaaattc aaaagatcat agacaaacgg cgttgcgccg agcatacagg
     5701 agcaacgctg ctgctcaagc agcgcgagac acgcatcagg agtgaaaata tctaacaaca
     5761 cgctgcgagc gccaattaag aatggtgccg ttacgccatg cagaaagccc gttgcgtgac
     5821 caagtggcgc aggcatcata aagacatcct gccaggttag attcagccgc gcgcaataag
     5881 cccgctcact ggcgagaata ttgttatgcg taagcatcac gccctttggc agaccctcgg
     5941 ttccggaggt aaacagcacc gcagctaatt catcgccgtg ggtcgttatc gccgtggtca
     6001 gtggggtatt gtcggcgata atctgactta atgagaggga agaggtggcg ggagccagtt
     6061 tgtccacgcc gacaatttgt tgtagttgtg gaagctgatt ttgcagcggc aggattaaat
     6121 ctaccggacg cgtttgttta aacaacgtcg gtgtaaagaa catttttgcc tgacacttat
     6181 taagcaccca taccagttct gcttcccgcc aggaaggcaa cagcggtacg gaaaccgcac
     6241 cgattttcag gcaggcaaga tagataacgg taaattcaca ccagcctggc agttgaaatg
     6301 ctatgcgatc gcctgattca atacccttcg ctaacatcca gtttgccaga cagctcgcgg
     6361 cgtgatcgag cgcgctatag gtgtacgatg ctccatgatt atcgaccacg gcaattttgt
     6421 ctggcatcgc acgagcggtc tgctgccagt aatcggccag cgaagcatcg ccccatagcc
     6481 cttgctgacg atacgccgca cgacgttgtt cgttaaacgt taatgtcact ttcattttgg
     6541 actctcgaaa cagaacatca ggcccgagat gcgggcctgt gggatgcatg attagccgta
     6601 gcgaaaatca ataccgaagg tgcctgcggg atattgccac tgttcgagaa tagtgttacc
     6661 gcacagcacc cgacaggtgc cgcactccag acaaccggcg gaatcaaaat gaatgtttcc
     6721 tgcgtcatcc tgcttataaa gtccggcagg gcaggctttc attaatttac ggaattcatt
     6781 gatatcggga tttgccgcca aaatgatatg cgggtggccc tcatcaacat ggaatttatt
     6841 gacgcctaat ttgatgtcga cgttaaccgt agcgttctgg ctcatagcgc ggttgctccc
     6901 ttaatgccat ctttcagcaa gttgatcagc ccaattttct tcgcgtgtcc catgatcatt
     6961 ttgcgtaccg gctggttagg cttaccgtca atggtgaaca tctcgttcat gatgtcggct
     7021 accattcgtg ggtattggct aaacaggcgc gggttttcca tcagcgccgg gatcttgcga
     7081 aaatgctgca tatcacgcat gacgcagctt tgttccagct cacgtttgta ttgcgccaga
     7141 ctgctggcgg agaaatccgc gcgttctttg gcggcgatca ctgttgtggc ggcagcctga
     7201 gccgatgcaa tggctaaatc catgccgcgg accgtaaaac ccaaattcag gcagaagcct
     7261 gcggcgtcac caacgatcat cacgccatcg ttaaccagtt gcggtaccat cgccagaccg
     7321 ccttctggca ccatatgcgc ggaatattca agcagtttgc cgccgctaat cagcgggcga
     7381 atggcggggt gttgtttaaa atcttccagc atttgcggca cgcttttttg cgcatgggcg
     7441 atatcagcca atccacaaac cagccccaag gatatggaat ccttgttggt atagagaaat
     7501 cccccgccca tcaggccgtc agaaggggaa ccggcaaaca gccaggcggc accttcctcg
     7561 cccgtaatat taaagcgatc gttgatctgt tctggggtga ggccaataac ctctttaaca
     7621 ccaacagcgt aatgatgcgg atcggaagcg ggaaccattc ccagcgagcg gccaagcatc
     7681 gagttaacgc catcggctaa aatcaccaca ttggcttcga gaatatcatc cccggcctgg
     7741 actccagtga ccttgtttcc ttcacgaacc aacgcatcga cgcgaactcc cgggataaac
     7801 tgtgcgcccg cctgctcggc ttgttccatc aaccacgggt ccagacgatt acgcaatacg
     7861 gtataagatg cgtgttgcgg aacatctggt tgctcgcggt gaaaatcgag ggtaacggcg
     7921 ctctcttcgg ttaagaagga gattttctcg cgtgtgacct tgcgttctac cggcgctgat
     7981 gctgcaaagc ctggaatgat tgcttcaagt gtgtgggcat aaagacgccc gccggtcatg
     8041 tttttacatc cggcactgtc gccgcgttct atcaccagga tatccagccc ggctcgcgcc
     8101 atgaccagtg cggcaacgct accagcaacg cccgcaccga ccacaatggc atcaaatttg
     8161 tcatccgaca tgcgttatgc tcccttcccg ctgcgtttac agcgggagat gtggttgaaa
     8221 ggggaattag cggctcaact ggctaatcag ggcagggacg actttgtaga tatcgcccac
     8281 cagaccgtag tcggcatagt tgaagattgg cgcattttta tctttattga tggcgacaat
     8341 cacttttgcg ccgttgccgc caaccatatg ctggatctgc ccggagatcc ctagcgtcag
     8401 gtagagttcg gatttcagca acacgccgga gacaccgata taacgttcac gctccatcca
     8461 gttctcgcct tcggcaattg gacgtgaaca gccgacttca gcattcagca ccgccgccag
     8521 ttcgtggacc attttcaggt catcctgcgc tgccagacca cgaccgacgc caaccacacg
     8581 ttttgctttg ctcaggtcca cgctgcttgc ggctttcgca cggcgttcgc gacagagaat
     8641 ttcatgacgc ggagcaacgt aaggtaccgt ttctgtcggg cactgatgag aggtatcact
     8701 ggtgcacggt tcctgaacac cgggtgcaag ggtaatgatc gccagcgagc tgttgatctt
     8761 ttcctgagcg aacgctaacc cgccatacat ccggtgttcg gcgcaaatgt gaccatcgac
     8821 aatatccacc gccgtggcat cgttcaccag cgccgcattc agttgcacac ttaaccgtgc
     8881 tgccagcgct ttaccacgtt tcgtcgcggc cagtagcaac atagcggggt gtttatcttt
     8941 cagcagagca gcaatgcttt cggcgtaatt ttcagtgcgt tgcagcgcgt cgttttgctc
     9001 aagaacataa atacattttg gaccataagg cataactgcc tgcgcctggt cggtattttg
     9061 tacaatggca tacacctgtt ggccccattg ctgagcgccg ccaaacagtt cagcataacg
     9121 ttcaggatta tcgctaaaga cccagacgct gtttaattga ctcatcgttt cttctccgta
     9181 ggcttcagtt cagggctttc ttcagatgtt ccgccagctc ggcaatggcc tccggcgaat
     9241 cgttatcgag aatgatgtgc ttacgttctg tttgcggcgg tacgcgaatg ccggtcagtt
     9301 cagcaagtgg tgcgctctgg ctccagccaa tatcacttgc ctgccactga tttaccggtt
     9361 ttttacccgc gccgagaatg gctttcatcg aaggaatgcg tggcacgtta atatcggagg
     9421 tgacgcagag cacggctgga acagagagtt caataacttc aacgtcatct tcaagcgtgc
     9481 gttcaatcac cagtgtattg ccctgacgct gaatagcact cactgcatta atcaccggaa
     9541 gttgcagaat ttctccgacc agcaagccaa cctgctgggc ataaaggtcg ccggaacctt
     9601 taccaaagat cagtaaatcg aagccgatct tttcaactgc tgccgccagc gcttttgcgg
     9661 tatcgagagg cagtgcatgc tcaagttgtg catcctgcac catatacagg ctgtgcggcc
     9721 cgcgggatag cacgtctttg cgcactttcg agttctgcaa caatgagccg ccaacggtca
     9781 gcgcggctat ctcatcgtca tctgttgcaa gctggcttgc agcttcaatg gcattgagat
     9841 cgaactggct gattttggcg tcggcattgt cgaaattcag ggtgtattct ggagtgacaa
     9901 caatgtcctg ttcttcaggc accagcttaa agcaggttat tattttcatg atttctcctg
     9961 tgagtcgcat acggaaccat ctccgatgga aggtaaaaat ggcctgaaaa tagtgtgcga
    10021 tagcggtgcg gggttttcga cggagcaagc tgttttcctt gccaacaatc ttgcaaaaaa
    10081 aatgcgatcg ctaaaacaaa agtgcaattt gcggcaatat agttattcca tgaccgtaca
    10141 atcttgcatt cagtgtaaat aatcttgttt agctctcgct aaacgcggtt gtataaccgc
    10201 aatacacgcc gtattttatc cggcatatat tgtttttgct tgctcatgga atagggaggt
    10261 tgtcatgtat caacgctgtt ttgataatgc cagtgaaacg ctgtttgtcg ctggtaaaac
    10321 gccacggctt tcacgttttg catttagcga tgatccaaaa tgggagtctg gacatcacgt
    10381 tcatgacaat gaaaccgagc tgatttacgt caagaaaggg gtggcaaggt ttaccatcga
    10441 ttcttcgtta tatgtcgcgc atgcagatga tatagtggtg atagaacgcg gcaggctgca
    10501 tgcggtggcc tctgacgtta acgatccggc aacgacgtgt acttgtgcgc tgtacggctt
    10561 tcagtttcag ggggctgagg aaaatcatct actgcaaccg cattcttgtc cggtaattgc
    10621 cgcggggcag ggaaaagaag tcattaaaac cttatttaat gagctaagtg tgattttgcc
    10681 gcaaagtaaa aatagccaaa catcttcgtt atgggatgca tttgcctata cattagcaat
    10741 tctttactac gaaaacttta aaaatgctta tcgttcggag caggggtata ttaaaaaaga
    10801 tgttctgata aaagatattc ttttctatct gaataataat tatcgcgaaa aaatcacttt
    10861 agaacagtta tcgaaaaaat ttcgtgccag cgtcagttat atttgccatg aatttaccaa
    10921 agagtatcgt atttccccta ttaactatgt tattcaacgg cgtatgacgg aagcaaaatg
    10981 gtcactcact aatactgaat tatcacaggc agagatctcc tggcgtgtgg gttatgaaaa
    11041 tgtcgatcac tttgccaaac tgtttttgcg ccatgtcggc tgttcgccca gcgattaccg
    11101 caggcaattt aaaaactgtt ttgcggaaca agaaatccta tctgaatttc ctcaaccggt
    11161 aagtcttgcc ggataaatct ggcggcgcag tttgttaaga agctgcgccg cctgcagatt
    11221 atttgttctg atagtctttc aggatctgcc gacctgctac gtaaatcata atttcgtctg
    11281 taccgccgcc aatacgttca caacggacat cacgccagaa gcgggagacg cgcgcctcat
    11341 cggtatagcc cagaccgccc atgatttgaa tcgcatcatc aatgacttcc attgcggtac
    11401 gtgcacagta cagttttgcc agcgccgcgc tggtgcgcag tgattgatgc tgatcggctt
    11461 gccatgccac tttcagcacc atgttgcgca tgttgtcaat cttaatcgcc atcagcgcca
    11521 gtttttcctg gatcatctgg ttatgaccaa tgggtttacc aaaagcgata cgctggttgg
    11581 catagcgagc ggcatcttca aaggcacact cggcaaagcc ggtgctgcgc gcggcgttga
    11641 tcaggcgctc catctcaaag ttgtacatca cattgaggaa acccattcct tcttcgccca
    11701 ccatgtcgct ctcttcaact tccacgttgt cgagatagac ttcgcaggtg ctgagcatat
    11761 gccagccgat tttatgcagc gggttaatct taatgccggg cttactggag tcgacccacc
    11821 acagggtgaa ggcttttttg ggatctttcg gttgcggatc gcgcgccagc accagcatat
    11881 acgggtactc tttcgcaccg gtaataaagg ttttctgtcc gttgatgtaa accttgccgt
    11941 ttttacgcgt gtaagtggta gtggcactgt tgttatctga gccagcacct ggttccgtca
    12001 acgccagggc ataggcggga tcacccgttt ccagggtgct ttctgccgtt ttacgtagct
    12061 gctctgcaga accgaaacgg cgcatactgt gaatacattg accgttggta attaaaaatg
    12121 ccggagcacc gcattttgac acttccatca gcgccagcat ttgggtgacg taatcagcag
    12181 ggataccacc aaattcttcc ggcacgccaa gcatggaaat accgttatcc gccagtgccc
    12241 gcataaactc acgcggatat gtcccgtttt gatcgcaggt gcggaaatac tcttccggaa
    12301 agttagtcgt aatcagttcg cgaatactgg ccagcagcag ttcttgttct tcagttaaag
    12361 aaaaatccat cgtgatactc ctgttaatga gccgcttcag gcaggacaaa acccatcgcc
    12421 gcatcgataa ataatctttc gtccatcagt ttgagttctg gcgaaatcac tggggtgaaa
    12481 tccattttgt cgagaatatc tttttgtaaa tcgacgccag gggcgatttc gattaaatgc
    12541 agtccgtctt ctttcagcgt gaatacggcg cgctcagtga tataacgaac atccagccct
    12601 cgctcgagag cgatttttcc gctaaaagta atttccggta gttcccgaat aaatttcttc
    12661 acccgtcctt cctggacgat attcaatttg ccatcggcaa tttctgtttt taaactgccc
    12721 gcagttaacg tgccgcagaa aataattttc ttcgaggtgg cactgatatc aataaatcca
    12781 ccggtaccca tgattttgcc attgaattta tgcacgccga cgttaccgtg ctggtcgact
    12841 tcagcaaaac tcaaataaca aacatccaga ccaccaccgt gataaaaatc aaactgggac
    12901 gtcatatcca gaatggcacg ggtattcacg ttcgagccaa aggcgatacc ctgtgaagta
    12961 attccaccaa tcggacctgt ttctaccgtc agaataaagt catcagcaca accttcttcc
    13021 cgggcgacca ggccaatgcc gtcagcaata ccgacgccga cattccccac cgcgccttta
    13081 cgcatttcga ataatgcacg ccgtgcaact aatttacgtt ggtttagggg caggctaagt
    13141 ttggtgctgt catcaagggt gaagtcacca gaaataaagc ggttaaccgg cgcaccgcca
    13201 tatagttggc tttgatccgg atcgaccacc acaatatcca ccagataacc cggaatacgg
    13261 acagatttag gatgcagtgt ggctttctta accattttct gcacctgcat catcacaata
    13321 ccgccgttat tgtgcaccgc ctgggcaata accaatgcgt cgagatacat cacctcatct
    13381 tcaaaagtgg cgtagccttc actgtcgcag gtggtagcgc gaatgaaagc aatatctggc
    13441 gcaatcgctt tgtaatagag atattctttg ttatcaaact cgaccagttt aatcaggtct
    13501 tctttagtga cttcattcag tttgccgcct tgctggcgtg gatcgacaaa tgtcccgatg
    13561 ccaatatcac taataatacc aggctggtgg gctgcggcgg cgcgtaaggt ttgtgtaagt
    13621 acaccttgtg gatagttata agcaataatt ttattttgtt ctgcgagatc agaaatacgc
    13681 ggcgattgtc cccagtgacc gcataatgcc catttcacca gaccttcttg cgccagcgga
    13741 ctaataccgc ggtcggcgcg atcgccaagc cctgttggac taataatcga taaattacgt
    13801 ggcgtttgag tctgtttata tttatcagca agagcagtaa ttaacgtggt ggcttccaga
    13861 ataccgccgc cagcgcctaa cacacaaagt gttgcttcgt cgggaatata attcaccgct
    13921 tcctgtgccg acaggaccgg cactcgtcca ttaatacgag gtggttttac aggtttcatt
    13981 gttaattcct gattcaggtg tgattttccc tacgccagat ggataattag cgcacagaga
    14041 ctcacgaaat atttttatta aatacggttg aatatatttt tttagttcgg cgggggagga
    14101 tattcccgcc gaaatattat tgcttatgcc tgatgtaaaa tagttaatac tgtgcgcaaa
    14161 tcatttaccg agatttgccc tggcgcagag gcttttttta ccgcaccaaa agttgccgcc
    14221 gagccaaata cttcaccagc cagacgagaa attacgccag tttttgccat tgacatcgtg
    14281 atgattggac gatcggcata ctgctcctgc atctccaggg tcgcggcaag caacgccagc
    14341 acatcgctgg tactttgcgg catcagcgca atcttaggaa tatcggcgtc gaaggactgc
    14401 attttgcgca gacgggcaat gatttcttcg gcttccggcg ttttatggaa gtcatggttg
    14461 gacatgacaa ctttcacatc atgcgcgtgg gcgtaggcga cggtttcttt aacctgatca
    14521 tcgccggtaa ataactccag atcgatcata tcaaccaggc cgctgtcgat ggctgcacga
    14581 ttgagagcaa tataagcctc ggtggaaatc gcctgctcgc cgccttcttt ggcactgcgg
    14641 aaggtaaaca gcagcggttt ttctggcatg gtttcacgga gaatttttgc cgccgccatg
    14701 acagactcca cattggagag gtcggcaaag tggtccacac gccattccag aatatcaaag
    14761 tccgcttcac gataggcgag agcttcggat ttcactcggg cgatatcttt cgccatcagc
    14821 gagacgatga ttttaggtgc gcccgtacca atgacgagat cttttacagt tacggttttc
    14881 attttttacc ctttctgcac gcggtcagcc tgtcaggcac cgaaccccat gacctgttta
    14941 acatattcca gagggaaatc tttgccagtc cataacgtga actgttcagc cccttgccac
    15001 aacaacatgc cgtatccatc aatcgttttg caaccagctt gttgcgcctg ctgcaataac
    15061 ttcgtcatat gcgggttata cacgcattca gtgaccagca gtcccggatg taacagactg
    15121 atatcattaa ccaatgattt attctcaagg ggtttcatac ccacttttgt gccattggtt
    15181 aaaatgtcgg cggaagccag ggcttcagca aaggcttgct gatcggcgag atcggtaacc
    15241 gtgacgacac aatcggtgtt ttcgttaacc cgctgcgcga aggcgagggc tttatcgaag
    15301 aacttatccc gacggttaaa gagtttaatt tcttttaaac cttcaattgc cccctgcgcg
    15361 ccaatcgccg ttgaggcacc tccggcccct aacagcacca ttgttttgcc tttgatatca
    15421 aaaccgctct ctttaatggc gcgaatatgg cccgtgccgt cggtgttata gccgcgcagg
    15481 tagccatcat cattaacgat ggtgttgatg gcacccacca gtttggctgc tggcgttaat
    15541 tcatcaacat attcacacgc cagttgtttg ttcggcattg atacaccagt tccgcgcatt
    15601 tttagagctt ttaatccttc aattgctccg ggaaagctat cgttatccac ttcgaaggcc
    15661 atataggtaa atggcaatcc cgctttttct aaggctttat tctgcatttc aggcgataaa
    15721 ctgtggcgga taggataagc catcaaccca atcaattcgt attttgcggt aacatccata
    15781 attaactcct ttaacctcta tgcttaattg tgtttaaccg ggtgctaaat ttgcgctcgc
    15841 caaaccgcac atcattctca ggaataataa aaacgcggta atagcggata aaaacaataa
    15901 ttgcggtaat cagggccagc agcgcgaaag taaaatcgag aacaatgata tattgcaggc
    15961 cgatgttcga cagataaccg gtaatcagcg gaataacaaa gttagccagt ccacccatca
    16021 tcatataaat actggtgact ttggctttgc tttttgggaa aaactctgac atgaccgaaa
    16081 caccgagctg taaaatgccg ccagctgctg agaaaccgat aacaaaggcg ccggcattac
    16141 acaccagtgg agaagggtac aggtagataa tggctgctgt tattgttgcc agtgcagagt
    16201 taaatacatt agcccagatg ggccggacca tttttttcag tagtgcggca aaaataaaga
    16261 cacagaccag cgagcccata ctgtaataag agatggtttt taatgcctca gcttctgaca
    16321 taccagcaaa agccatcgca tatttgggca tccacaccac aatcacataa aaggtcgaga
    16381 atgcggctac accgaacagt accgatgaaa caccttccag ccagactaac ggtttgctgt
    16441 tcatttgcgg taattcatta gctacgctgg cgtccaccaa ctggctgggg aatttgcttt
    16501 tcaacagcat cagcgtgatc agtacaaaca gaatacccgg aataatcagc ccatagccgt
    16561 accagatatt attgagcaac atatagctca ccagcattgg gtagaacatt tgcccaaatg
    16621 acaccatcgc tttaaccagt atgaccgccg aaccagaggc tttcggaaag cattccatga
    16681 gcgcggggta gccacccgta tccagcgctg agttagcgat acctacgcac actgccagac
    16741 cgtaggcgag agttaaattc gggcaagcgg gaataccaaa gaagaatagc agatacatta
    16801 ttactgccat taatatcacc gcccgacgac caaacttatc ggagatcaca ccgaagaata
    16861 aaatactgat caatcgcccc aaaccgatac cggaaattaa gtaggcaatg cccgcgttgt
    16921 cagtggaaaa cttttccgcc agagatgaca tattttgggc aagcgtaata acactaatgc
    16981 cgtgcaggaa gtagctgaag taaatacaaa gaacagccag gataaatggc gtgctgaaag
    17041 ccttattttg agacattttc acagctcctt gctgaaagat aatttcgtta ccgtacgtat
    17101 tgttctccga cggtttacat aattactata ctgaagatga ttttccctgg gtacatggca
    17161 taaagtttat gtttccagga atattgcatg tcatagtttg tttttgatcg cgattttcct
    17221 tagttagcgc agaaagagtt atttatccgt aagatcttgc atgacctgaa aagaatattg
    17281 cattacccac ccggagcgac attttccttt agcgagtgat gccgcgtttt tttgcggctg
    17341 cgtaagccga taaacagtgc cagtaaaaaa ccgatggcag cgatggcggt atcgaaccac
    17401 ataatatcgg caatacttct ttgggacaga tgagccgtaa tcaacggaat agtaaaggtc
    17461 gcaatactgc ccgcactgta atagatccct gtagctttac ctttagcgta agggaaacgt
    17521 tcagccatta acgtcaggcc aatttgcaca acacctccag cagaagtaaa accaatgaca
    17581 aaagaaaata ttatcaccac ataaaatgtg ggatgcaggc agacggtaaa cagagcaata
    17641 aatgagataa aggtgtacag catcagtaat gttgttgggc gaacggtatt acgaatgagt
    17701 ggagcggtaa taaatacaca aagcagcgaa cccacggtgt agatactgag tagtttgatc
    17761 gacatagtgt atgacatgcc tgcaacaaat tgtccgtact gtgccagcca ctggctaacc
    17821 agataaaacg ttgccattga gatatagcca tataaggtat aactggctaa atcgataatt
    17881 gaacagcgat gttccgtaga gctggtggtt ttctttatga caggtaagcg acgacccgga
    17941 tggggtggga acgtacaacg gtataaaaac agagcgttaa taaacataat gcctgcagca
    18001 atcataaagg accaaccgaa ccacagttca gcccacacta acaggctaat gattagcggt
    18061 aataaaaatt gtccgctgga aacaaatgct ttaattaaaa tattggctgt cccaggtgag
    18121 cgtggaaaag cttccatcaa actgggataa gtgcctgcat cgagaaaact gtttgccatt
    18181 cccgccagaa agccaaaaac ataagcgata atgatgttat tggtctgcag gatgccaaaa
    18241 aagaaggcca tatagcagca catcccgagc atgataaaag ggcggcgacc aaagcgatcg
    18301 gataataatc ctgcaaaaag caagacactt aatcgaccaa tgcccagcga tgagataact
    18361 atcgagacac ccgcggcatt agactgccaa agtgtctcca gcgaggccat attcaggctc
    18421 atcaaaatga cgcccatacc atgcaccagg taattaaaat acaacccaag tgcggtaggg
    18481 taatagggat ttttcatagg ttctacttag caaaggtgct ttaaaaagtg tagcgatgtt
    18541 gtgtcagtat ccgttcatta ttgttgtaac aacgacaatt cctttttgtg gagaaattaa
    18601 caaagccgct ccagatcgtg tgcatataat tcagcggcta ccgattgata gatcttccag
    18661 tcaataaaat tgccatcagg gtagacttgt tgaaacgcgg ttaagtcggg gaaaaaacgc
    18721 atggtgttgt tgccgatgcg gttatttatt ttctctatga tcgcatgaag atgttttttt
    18781 ctttgctgac gcatagccaa cagttgctcg actacacaat caggaatggc acacctgcca
    18841 ttttcccatt gacgccagct ttcactgtca ccggtctggg caatccaggt cgcgcattca
    18901 tcaatagtca tggcaaaaat atggcggagt gcctggagtt cataagcgtt catcgtgtaa
    18961 tggctgcatg aaataaacaa taaaatataa ataacattat cagcctgctg acggcttgaa
    19021 gagagtcaca gtatcttgtg caacattatt ggaaagagtg aggggcgagg tagcgaagcg
    19081 gaaaaatgtt aaaaaaaagc ccatcgtggg agatgggcaa agactacaca cagcaattcg
    19141 ttgtttcact caggggattt ccatgcttat aaatcaatat gttgatttat aaccgtgagc
    19201 taatagtagg catacggact ttttgcgtcg atcagattcg tctcattagt ttaaaaactg
    19261 taaagaattt gcgacaggta ctaccagctg aatggcggta agcaggaaaa tatggggaag
    19321 agcaagcaaa tatcaattag cggtggttaa caatcaggcg gcagagccgc ctgatgagaa
    19381 attacttatt cggtttctgg atttcgccaa gttcttcgag aatttcttcc ggttgatccg
    19441 tcggcggtgg gacctcttcc acccacgcgg caaacagacg ccaggaaacg gctaacagaa
    19501 ccggaccaat aaacagaccg atcatcccga aagcaatcaa accaccaata acgccagaga
    19561 ggatcaggat cagcggtaaa tcggcaccca tgcgaattaa cattgggcgg atgacgttat
    19621 ccagcgtgcc aaccacaccg ctccacacta acaataccgt tccccaggtg gtatcgccag
    19681 tccagtagag ccagataatc gccggaatca gtaccggcaa cgggccaagc tggacaaggc
    19741 aggaaaggat cattaacacc gttagcaaag ttgcataagg tacgccggat acggcgaggc
    19801 cgataccgcc aagcactgcc tgtactaacg ccgtcaccac cacacccagc gccaccgcgc
    19861 ggatagcctg tgccgccagc agtacggcgg catctccgcg aacgcctgcc agacgggtcg
    19921 caaaatggcg aatgccttgt gccacctgtt caccgcgcca gtacagcagg gcactgaaga
    19981 gaagcatcag cgcacaatgc accataaagc gcccgatatg cgccgcttgc ccaacgaacc
    20041 aggtagtggt ggtgccaata taagggcgga ctttcgccat gatcgccgtc ccccccatat
    20101 ccagcaagtt gtgccagcct gcatacagct tcgcgccaat caccggaatg gtattaagcc
    20161 acgccagatc gggtaacgtc atgtcaccgc tggaaatggc tttaattagc gggccgctgc
    20221 cgtcgacgat actgttaacc agcaaagcaa tagggatgat aaacaccatc actaataaca
    20281 gcgtcatcac cagaacggcg agagagcggc ggccaaacat gatcttttgc aaacgtaaca
    20341 ataccggcca ggtggcgata accaccgtac cggcccatgc aaagccgaga ataaagggtt
    20401 gaacaatcca cagacatgcc acaatcatga tggctaaaaa cagcaccgaa agcagaattt
    20461 gtgcgacatc cctgggctga cgaacattta ccataactac ttttcacctt ttttgtgcgt
    20521 caaatcgtcg acgcgacgtg aacacgcgaa actcacctgc ataatgatga gcaatttcag
    20581 cggtttttaa caggccgatt ctgcccttaa ttctgatggg cgtgttagaa aaaaatgtgg
    20641 tacaacaact caggcaacac gcaaacgatt tactcgtcgt atttcaagtt gcatatgctg
    20701 cggctacgtt cgttcacccc agttacttac tgttgtaagc tcctggagtt cactcgcttg
    20761 ccgccttcct gcaactcgaa atccatagag taaataaaca tcacctttct gtgcgctatt
    20821 gcagccgcct gaaagaacag tgtatcgacc tacatcacag accgcaggaa agggtcaata
    20881 taatgattcc acagatttcc caggcacccg gcgtcgttca actggtgctt aattttttgc
    20941 aagagctgga gcaacaaggt tttaccggcg atacggcgac aagttatgct gatcgtctga
    21001 caatgtcgac cgacaacagt atttaccaac ttctccccga tgcagtggta tttccgcgtt
    21061 caaccgcaga tgtggcgctg atcgcccgtc ttgccgcgca ggaacgctat tcatcgctga
    21121 tctttacccc ccgcggcggc ggcaccggca ctaacggtca ggcgctcaac cagggtatta
    21181 ttgttgatat gtcccgccat atgaaccgca tcatcgaaat taaccctgaa gagggctggg
    21241 tgcgcgttga ggccggggtg ataaaagacc aactcaatca gtacctgaaa ccgttcggct
    21301 acttttttgc accagaactt tcgaccagca accgggcaac gctcggcggg atgatcaata
    21361 ccgatgcatc cggtcaggga tcgctggtct atggcaaaac gtcagatcac gtacttggcc
    21421 tacgtgcggt attgttgggg ggcgatattc tcgatacgca acctttaccc gtcgagctgg
    21481 cggaaacgct gggtaaatcc aataccacaa tcgggcgaat ttataacacg gtttatcaac
    21541 gttgccgtca gcaacgccag ttaatcatcg acaacttccc caaacttaac cgctttctta
    21601 ccggttacga tctgcgtcat gtctttaacg atgagatgac cgagttcgat ctgacgcgca
    21661 ttctgacggg ttcagaaggg acgctggcct ttattaccga agcgcggctg gatattacgc
    21721 gcttgcctaa agtgcgccgt ctggtgaatg tcaaatatga ctcttttgac tctgcgctgc
    21781 gtaacgcgcc gtttatggtt gaggcgcggg cgctttcggt agagacggtg gactcaaaag
    21841 tgctgaatct ggcgcgggaa gatattgtct ggcattccgt cagcgagttg attaccgatg
    21901 tgcctgacaa agagatgctc gggctgaaca ttgtggaatt tgctggtgat gatgaggcgc
    21961 tgattgacga gcgggtaaat gcactctgtg cgcggttgga tgagctgatc gccagtcatc
    22021 aagcaggtgt catcggctgg cagatgtgcc gtgagctggc gggggttgaa cgtatctatg
    22081 caatgcgcaa aaaagccgtt ggtctgcttg gcaatgccaa aggtgccgct aagccaattc
    22141 cgtttgctga ggatacctgc gtaccgccgg aacatctggc cgattatatt gctgaatttc
    22201 gcgcgctgct cgacagccac ggcttaagct acggtatgtt cggtcacgtc gacgcaggtg
    22261 tcttgcatgt ccgtccggca ctggatatgt gcgatccgca acaagagatt ttgatgaagc
    22321 aaatctctga tgacgtggtg gcgctgactg cgaaatacgg tggtttgttg tggggcgagc
    22381 acggcaaagg ttttcgcgct gaatacagcc cggcgttttt cggtgaggaa ctttttgcag
    22441 aactgcgcaa agtgaaagcg gcatttgacc cgcataaccg actcaaccca gggaagattt
    22501 gcccgccaga aggtctcgat gcgccgatga tgaaagtgga cgcggtgaag cgcggtacat
    22561 tcgatcggca gatccccatt gcggtacgcc agcagtggcg cggtgcgatg gagtgtaacg
    22621 gcaacggttt atgcttcaac tttgatgccc gtagtccgat gtgtccgtcg atgaagatca
    22681 cccagaaccg gattcattca ccgaaagggc gcgcaacgct ggtgcgtgaa tggctgcgtt
    22741 tgttggcgga tcgcggcgtt gatccactca aactggaaca agaactgcct gaatccggcg
    22801 tcagcttgcg gacgttaatt gcccgcacgc gcaatagctg gcatgcgaat aaaggcgaat
    22861 atgacttctc acacgaagtc aaagaggcga tgtcgggctg tctggcctgt aaagcttgtt
    22921 cgacccagtg ccccatcaaa attgatgtgc cggagtttcg ctctcgtttt ctgcagctct
    22981 atcacacccg ttatttacgc ccgctgcgcg accacctcgt cgctacggtc gagagctacg
    23041 cgccgctgat ggcacgcgcg ccgaaaacct ttaacttctt cattaaccag ccgctggtgc
    23101 gcaaactctc ggaaaaacat atcggcatgg ttgatttgcc gctgctgtcg gtcccctcgc
    23161 tacaacaaca aatggtgggg catcgctcgg caaacatgac gctggaacag cttgaatctc
    23221 tcaatgcaga gcagaaagcg cgcacagtgt tggtggtgca ggaccccttt accagctatt
    23281 acgatgcgca agtggtggcg gattttgtcc gtctggtcga aaaattaggt ttccagcctg
    23341 tgttactgcc attttcgcca aatggcaaag cccagcatat taaaggtttt cttaatcgtt
    23401 ttgcgaagac ggcgaaaaag acggcggatt tcctcaaccg tatggcgaag ctgggtatgc
    23461 caatggtggg cgtcgatccg gcgctggtac tttgttatcg cgatgaatat aaactggcgc
    23521 tgggcgagga gcgtggcgag tttaacgtct tactggcgaa tgaatggctg gcaagcgcac
    23581 ttgagtcaca gccagtggct acagtcagcg gtgaatcatg gtatttcttt ggtcactgta
    23641 ccgaagttac cgccttgccg ggtgcgccag cacaatgggc cgcgatattt gcccgttttg
    23701 gcgcgaaact ggaaaatgtc agcgtgggtt gctgcggcat ggcagggact tacggacatg
    23761 aagcgaaaaa ccataaaaat tcgctcggga tctatgagtt atcctggcat caggctatgc
    23821 agcgactgcc gcgtaaccgc tgtctggcga ccggatattc ctgccgtagt caggtcaaac
    23881 gggttgaagg cacgggggta cgccatcctg tgcaggcttt actggagatt attaaatgat
    23941 atggaaacga aaaatccccc tggaagcact gaatgccatg ggtgaaggaa acatggtggg
    24001 gttactggat attcgctttg aacatattgg tgatgacacc cttgaagcga caatgccagt
    24061 agactcgcgg acaaagcagc ctttcgggtt gctacatgga ggtgcatccg tggtactggc
    24121 cgaaagtatc ggttccgttg ccggttattt atgtaccgaa ggtgagcaaa aagtggttgg
    24181 tctggaaatc aatgctaacc acgtccgctc ggcacgagaa gggcgggtgc gcggcgtatg
    24241 caaaccgttg catctcggtt cgcgtcacca ggtctggcag attgaaatct ccgatgagaa
    24301 agggcgtttg tgctgttcgt cacgattgac gaccgccatt ttgtgacgtt gtcaccagaa
    24361 aagtgtgacg catattgctt ttggtcatta tctgcacagt aaacggtggt atactgtgca
    24421 ggtcttgtct aaaagcgagg atgctatgtc tactcaactt gatcccaccc agctggccat
    24481 tgaattttta cgccgtgacc agagcaatct ttcacctgcc caatatctta aacgtctgaa
    24541 acaactagaa cttgaatttg ctgacctgct tactctctct tctgctgaat taaaagaaga
    24601 aatctacttt gcctggcgtc tgggcgtgca ttgatcctga cttaatgccg caagttcgcc
    24661 aaatcgacct ggattaacct ccgtccaggt tcgatgacct tcttaatact gcttatctac
    24721 ccgcctgata ttgttcaaaa ataatactaa tcgcagtttg tgttaaaacg gcggcttagc
    24781 tttatgagat tattcttatc gccccttcaa gagctaagcc actgagagtg ccggagataa
    24841 gcgccggatg gggtagaaac ccttaagcct gtgtcgcaca gacttaaggg ttttcttatt
    24901 tctgaacgct gcactttttt acctatctct taaatagtca ttttcaatac aactttttct
    24961 ttttcatcac agccttaatg ctccttagat aataatcatt atctaacaat gagataccta
    25021 attcttagcg tgcctgttaa cccacacatc agggtctatg cttattaaaa gcaggcaaaa
    25081 agcatgtttt acggtaaagc ccctgccttt gctgggttga actgataatc attatcacta
    25141 acatgctgtt atacgctgaa agcgatgaag tgaggtaaat cgatggacat gcattcagga
    25201 acctttaatc cacaggattt cgcctggcaa ggcttaacgc tgacacccgc agccgctgtc
    25261 cacatccgcg agctggtggc aaagcagccg ggtatggtcg gcgtgcgctt aggtgtgaag
    25321 caaacgggct gcgcgggctt tggctatgtg ctcgacagtg ttagcgagcc ggacaaagac
    25381 gatctgctgt ttgaacacga cggcgcgaag ctgtttgtcc cgctgcaagc gatgccgttt
    25441 attgatggca cggaagtcga tttcgttcgt gaaggactta atcagatatt caaatttcac
    25501 aaccctaaag cccagaatga atgtggctgt ggcgaaagct ttggggtata ggcggtacta
    25561 tgtctcgtaa tactgaagca actgacgatg tcaaaacctg gaccggcggc ccgctgaatt
    25621 ataaagaagg attcttcacc cagttagcca ccgatgagct ggcaaagggg ataaacgaag
    25681 aggtggtgcg cgcaatttcg gcgaagcgta atgagccgga gtggatgctg gagtttcgtc
    25741 taaacgccta tcgcgcatgg ctggagatgg aagaaccgca ctggttgaaa gcgcactacg
    25801 acaagctgaa ttatcaggat tacagctact actcggcacc atcgtgcggt aattgtgacg
    25861 acacttgcgc gtctgaacct ggcgcggtgc agcaaactgg cgcgaacgcc tttttaagta
    25921 aagaggtgga ggcggcgttt gagcagttgg gtgttcccgt gcgggaaggc aaagaggtgg
    25981 cggtggatgc cattttcgac tcagtttcgg ttgccactac ttatcgcgaa aaactggcgg
    26041 agcagggaat tattttctgt tcctttggtg aggcgatcca cgatcacccg gaactggtgc
    26101 gtaaatatct cggcaccgtg gtgccgggga atgacaactt ctttgccgcg cttaatgcgg
    26161 cggtagcctc tgacggtacg tttatttatg tgcctaaagg cgtgcgctgc ccgatggaac
    26221 tttccaccta ttttcgcatt aacgcggaaa aaaccggaca gtttgagcgc accattctgg
    26281 tggccgacga agacagctac gtcagctaca ttgaaggctg ttccgctccg gtgcgtgaca
    26341 gctatcagtt gcacgcggca gtggtggaag tcatcatcca taaaaacgcc gaggtgaaat
    26401 attccacggt acaaaactgg ttccctggcg ataacaacac cggcggtatt ctcaacttcg
    26461 tcaccaagcg tgctttgtgc gaaggcgaaa acagcaaaat gtcatggacg caatcagaaa
    26521 ccgggtcagc gattacgtgg aaatatccca gctgcatttt gcgcggcgat aactccattg
    26581 gtgagtttta ctcagtggcg ctgaccagcg gtcatcagca agcggatacc ggcaccaaga
    26641 tgatccacat cggtaaaaac accaaatcga ccattatctc gaaagggatc tctgccggac
    26701 atagccagaa cagttatcgc ggcttagtga aaatcatgcc gacggcaacc aatgcgcgca
    26761 atttcactca gtgcgactca atgctgattg gcgctaattg tggggcgcat accttcccgt
    26821 atgtcgaatg tcgcaataac agcgcacaac tggagcacga ggcaacgaca tcacgtattg
    26881 gtgaagatca actgttttac tgcctgcaac gcgggatcag cgaagaagac gccatctcga
    26941 tgattgttaa cggtttctgc aaagacgtgt tctcagaact gccgctggaa tttgccgttg
    27001 aagcacaaaa actcctcgcc atcagtcttg aacacagcgt cggataagga ataaacatgt
    27061 taagtattaa agatttacac gtcagcgtgg aagataaagc tatcctgcgt ggattaagcc
    27121 tcgacgttcg tcccggcgaa gttcacgcca ttatggggcc aaacggttcg ggcaaaagta
    27181 ccttatcggc aacgcttgcc gggcgagaag attatgaagt gacgggcggc acggttgagt
    27241 tcaaaggcaa agatttgctt gcactgtcgc cggaagatcg tgcaggcgaa ggcatcttta
    27301 tggccttcca gtatccggtg gagattccag gtgtcagtaa ccagtttttc ctgcaaacgg
    27361 cacttaatgc ggtgcgcagc tatcgcggcc aggaaacgct cgaccgcttt gattttcagg
    27421 atttgatgga agagaaaatc gctctcctga agatgccgga agatttatta acccgttcgg
    27481 taaacgttgg tttttccggc ggcgagaaaa agcgcaacga tattttgcaa atggcggtgc
    27541 tggaaccgga gttatgcatt cttgatgaat cggactccgg gctggatatc gacgcattaa
    27601 aagtggtcgc cgatggcgtg aactcgctgc gtgatggcaa gcgctcattc atcattgtta
    27661 cgcactacca acgcattctc gactacatca agcctgatta cgttcatgtg ctatatcagg
    27721 gacgaattgt gaaatccggc gatttcacgt tggtcaaaca actggaggag cagggttatg
    27781 gctggcttac cgaacagcag taacgcgctg caacagtggc atcacttgtt tgaagctgaa
    27841 gggacaaaac gctccccgca agcacagcag catttacaac aattgctgcg taccggactg
    27901 ccgacacgta aacatgaaaa ctggaaatat acgccgctgg aagggctgac caatagtcag
    27961 tttgtcagca ttgcgggaga gatatcccca cagcagcgag atgccttagc gttaacgtta
    28021 gacgctgtgc ggctggtatt tgtcgatgga cgttacgtgc ctgcgctgag cgatgcgact
    28081 gaaggcagcg ggtatgaagt gagcattaac gacgaccgtc aggggttacc cgatgctatt
    28141 caggcggaag tgtttctgca tttgacggaa agcctggcac aaagcgtgac gcatatcgcc
    28201 gtgaagcgcg gtcaacggcc ggcaaagcca ttgctgttaa tgcatatcac ccagggcgtg
    28261 gcaggtgaag aggtgaacac tgcccattac cgacatcatc tggagctggc ggaaggtgcc
    28321 gaagcaacgg tgatcgaaca ttttgtcagc cttaatgatg ctcgtcattt taccggggca
    28381 cggttctcta tcaacgtcgc agcgaatgcc cacttgcagc atatcaagct ggcgtttgaa
    28441 aacccgctca gtcaccactt tgcccataac gatttgttgc tggctgacga tgccaccgca
    28501 tttagccaca gtttcctgct gggtggcgca gtgttacgac acaacaccag tacgcaactc
    28561 aatggcgaaa actgcacgct gcggatcaat agcctggcga tgccggtgaa aaacgaggtg
    28621 tgtgataccc gtacctggct ggaacacaat aaaggttttt gtaacagccg acagttgcac
    28681 aaaactatcg tcagcgacaa aggccgcgcg gtatttaacg gtttgatcaa cgtcgcgcag
    28741 cacgccatca aaacggatgg tcagatgacc aacaataatc tgctgatggg caaactggcg
    28801 gaagtggata cgaaaccgca gctggaaatc tatgcagatg atgtgaaatg cagccacggc
    28861 gcgacggtgg ggcgtattga tgatgaacag atgttctatc tgcgctcgcg cgggatcaat
    28921 cagcaggatg cccagcagat gatcatttac gcctttgctg ccgaactgac ggaagcactg
    28981 cgtgatgagg ggcttaaaca gcaggtgctg acccgaatcg gtcaacggct gccaggaggt
    29041 gcaagatgac tttttccgtc gacaaagtgc gggccgactt tccggtgctt tcgcgtgagg
    29101 taaacggttt gccgctggct tatctcgaca gcgccgccag tgcgcagaaa ccgagccagg
    29161 tgattgacgc cgaggccgag ttttatcgtc atggctacgc ggcggtgcat cgcggtattc
    29221 ataccttaag cgcccaggcg accgagaaaa tggagaacgt gcgcaagcgg gcatcgctgt
    29281 ttattaatgc ccgttcggcg gaagagctgg tgttcgtccg cggcacgacg gaagggatca
    29341 atctggtcgc caatagctgg ggcaacagca acgtgcgggc gggcgataac atcatcataa
    29401 gtcagatgga gcaccacgct aacattgttc cctggcagat gctttgcgca cgcgttggcg
    29461 cagagctgcg tgtgatcccg ctcaatcccg acggtacgtt gcaactggag acgctgccta
    29521 cgctgtttga tgagaaaact cgcctgctgg caattactca tgtctccaac gtgcttggca
    29581 cagaaaatcc actggcggaa atgatcacgc ttgcgcacca gcatggcgca aaagtgctgg
    29641 tggatggcgc tcaggcggtg atgcatcatc cggtggatgt tcaggcgctg gattgcgatt
    29701 tttacgtgtt ttccgggcat aaactgtatg gccccaccgg gattggcatt ctttatgtca
    29761 aagaagcctt gctacaggag atgccgccgt gggaaggggg cggttctatg atcgccaccg
    29821 tcagcctgag tgaaggcact acctggacca aagcaccgtg gcggtttgaa gccggtacac
    29881 ccaataccgg gggcatcatt ggtcttggcg cggcgctgga gtatgtttcg gcgcaggggc
    29941 ttaataacat agccgagtat gaacagaatc tgatgcatta cgcgctatca cagctggaat
    30001 ctgtaccgga tctcactctc tatggcccac aaaacaggct tggcgttatt gcttttaatc
    30061 tcggtaaaca ccacgcctat gatgttggca gttttctcga taattacggc attgctgtgc
    30121 gtaccggaca tcactgcgct atgccattaa tggcctatta taacgtccct gctatgtgtc
    30181 gggcgtcgct ggccatgtat aacacccatg aagaagtgga tcgtctggtg accggactgc
    30241 aacgtattca ccgtctgctg ggataacagg gaggcactat ggctttattg ccggataaag
    30301 aaaagttgct gcgtaatttt ttacgctgcg ccaactggga agagaaatat ctctacatta
    30361 ttgagctggg ccagcgtctg ccagaattac gcgacgaaga cagaagccca caaaatagca
    30421 ttcagggctg ccagagtcag gtgtggattg tcatgcgtca gaatgcgcag ggaattattg
    30481 aattgcaggg cgacagcgat gcggcgattg tgaaagggct tattgcggtc gtctttattc
    30541 tctacgatca gatgacgccg caggatgttg tcaatttcga tgtgcgtccg tggtttgaaa
    30601 aaatggcact cacccaacat ctcaccccat ctcgttcaca aggtctggaa gcgatgattc
    30661 gcgcaattcg cgccaaagcc gctgcactta gctaaactaa agggacagct ttcatccggt
    30721 tggctcacat tgccggatgg tcaatgttta cactgacctg cacaggagtg tttcggtttc
    30781 aggtgaacat aagcgccatc cgacgtttca gcgtgagtct ccggcaaata caggaggttt
    30841 acaatgaaac gcgcgtcttt gcttacactc acgcttatcg gcgcttttag tgccatccag
    30901 gctgcctggg cggttgatta tccgctacca ccaaccggaa gccgactggt tgggcaaaat
    30961 caaacgtata cggtgcagga aggggataaa aaccttcagg ccatcgcccg acgttttgat
    31021 actgcggcaa tgttgatcct tgaagccaat aacactatcg ccccggtgcc aaaacccggt
    31081 acgacgataa ctattccttc gcaactgtta ttacctgatg cgccgcgtca ggggattatc
    31141 gttaaccttg cagagctgcg cctttattat tatccgccgg gagaaaatat cgtgcaggtt
    31201 tatccgatag gtattggctt gcaggggctg gaaacgccag tgatggaaac gcgtgtgggg
    31261 cagaaaatcc ctaacccaac ctggacgcct acggcaggca ttcgtcaacg ttcgctggag
    31321 cgtggcatta aattaccgcc agtcgttcct gccggaccaa ataacccgct aggacgttac
    31381 gcactgcgcc tcgcgcatgg taatggcgaa tacctcattc atggcaccag tgcgccggac
    31441 agcgtcggtt tgcgcgtcag ttcagggtgt attcgcatga atgcaccgga tattaaagcc
    31501 ttgttctcca gcgtgcggac gggaacgccg gtgaaagtga tcaacgaacc agtgaaatat
    31561 tccgtggagc ctaacgggat gcgttatgtt gaagtacatc gaccactatc ggcagaagaa
    31621 cagcagaacg ttcagacaat gccatacaca ctgccagcag gctttacgca atttaaagac
    31681 aataaggctg tagatcagaa gttagtcgat aaagcgttgt atcgtcgggc agggtatccg
    31741 gttgcggtga gcagtggagc aactcccaca gccagcaatg cgccttcagt agagtcagcg
    31801 cagaatggtg aaccagagca agggaatatg ttacgcgcga cgcagtagtg gtaaatagca
    31861 gacaaaaaaa tggcgcacaa tgtgcgccat ttttcacttc acaggtacta ttacttgcgg
    31921 tatttagtag ccatgttgtc cagacgctgg ttagcacgag ctgcgtcatc tttagcagcc
    31981 tgaacgtcgg aacgcattgc gttcacgtcg ttgctcagct ggtcaacttt agcgttcaga
    32041 gtctgaacgt cagaagacag ctgatcgatt ttagcgttgc tggagcaacc tgccagcaga
    32101 gtagaaccca ggattaccgc gcccagtacc agtttagtag ctttcattat taataccctc
    32161 tagattgagt taatctccat gtagcgttac aagtattaca caaagttttt tatgttgaga
    32221 atattttttt gatgggaagg cacttatttt tgatcgttcg ctcaaagaag catcgaacgc
    32281 tgggttttag ataaaaaaat tgagagaaaa caggatgctt ccatcggatt catc
//
LOCUS       DAZFAB010000046        31921 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZFAB010000046 DAZFAB010000000
VERSION     DAZFAB010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31921
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.046"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..874
                     /gene="espN"
                     /locus_tag="ACHJWA_002434"
     CDS             <1..874
                     /gene="espN"
                     /locus_tag="ACHJWA_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspN"
                     /protein_id="HFO4309018.1"
                     /translation="LEMKQDRGGNRYSAVDLNSSKYGYYEKVGGGFYRYIESFNPISS
                     ETPNKIVYKGESVDLTKEPNSELYSGRYSINNKQVNVYFFRDADGTFYKSEGLHGGGV
                     IRYIDKPYSQLREGDIGYDEDLLDIYDDSPVLEDTLPALSSEIVPTPEHSIKQIYSKI
                     KEGHIELSDSDIILCRGTTGIQAENIVEYKTAGGLPDSNPNVKAPDEYMAQQQVRIGR
                     ILPEYTSDLSVADRFSREHYLIVVKVKAKYITRGSVTESGWVIDKTAPVEPLAIIDRT
                     FGMKENISMVNASK"
     gene            complement(1251..1406)
                     /locus_tag="ACHJWA_002435"
                     /pseudo
     CDS             complement(1251..1406)
                     /locus_tag="ACHJWA_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309595.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein GogB"
     gene            complement(1417..2645)
                     /locus_tag="ACHJWA_002436"
                     /pseudo
     CDS             complement(1417..2645)
                     /locus_tag="ACHJWA_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
     gene            2737..2955
                     /locus_tag="ACHJWA_002437"
                     /pseudo
     CDS             2737..2955
                     /locus_tag="ACHJWA_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2990..3103
                     /locus_tag="ACHJWA_002438"
                     /pseudo
     CDS             2990..3103
                     /locus_tag="ACHJWA_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308873.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="non-LEE encoded effector protein NleB"
     gene            complement(3183..3458)
                     /locus_tag="ACHJWA_002439"
     CDS             complement(3183..3458)
                     /locus_tag="ACHJWA_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspO"
                     /protein_id="HFO4309019.1"
                     /translation="MPFSIKNRFSSSQVHYPEISGPIKDKPASKNCILTSTTCNVDSY
                     TVYQKKACSFDMRPPGAGERTPKLKLSVTEMTWLSKTIETEIHNTKE"
     gene            complement(3519..4880)
                     /gene="espK"
                     /locus_tag="ACHJWA_002440"
     CDS             complement(3519..4880)
                     /gene="espK"
                     /locus_tag="ACHJWA_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspK"
                     /protein_id="HFO4309020.1"
                     /translation="MLPTSQLRPTGTFCSYSAETSADIKSEITPIQIEEARASGRLYI
                     KDCDIEYLPQLPNEITSVTIENCNNLTTLTGLPVNTQNLSVINCEKLQITDMPSTVKN
                     LHIELTDSPFIHFISEGIECLTVCHCYISGVPESVRYLEIKGSATDSIKNVPNGLSSL
                     SINSYNPENQARIDNLISPSLKTLSLTGCSNIILPEKLPESVTSVTIHAEQKTTWNIG
                     VEGMPDGLDLDLQNVLLSPDVVKAKNITFQGNALDVALHFREGDIVYGLSSPREKLVN
                     SIKLVNDFSKKDIITQNTLTNAVWDPRTPRKYKQDPLIKRALNEHERGIKFKQHLKNH
                     NNYNVTMADLSVYNRDKLWAKTSKAGLEFQTLTRNKTVIFCADELVNSLKLIANKSEG
                     YGQSITASELRWIYRNKDNNQIMKNIKFYLHGKEIPAERILDTPEWKDYRPKYSGSTY
                     KYS"
     gene            complement(5244..6107)
                     /gene="potB"
                     /locus_tag="ACHJWA_002441"
     CDS             complement(5244..6107)
                     /gene="potB"
                     /locus_tag="ACHJWA_002441"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFO4309021.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLARDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKATEIDN"
     gene            complement(6091..7227)
                     /gene="potA"
                     /locus_tag="ACHJWA_002442"
     CDS             complement(6091..7227)
                     /gene="potA"
                     /locus_tag="ACHJWA_002442"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFO4309022.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            7477..8703
                     /gene="pepT"
                     /locus_tag="ACHJWA_002443"
     CDS             7477..8703
                     /gene="pepT"
                     /locus_tag="ACHJWA_002443"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFO4309023.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            complement(8752..9873)
                     /gene="roxA"
                     /locus_tag="ACHJWA_002444"
                     /gene_synonym="ycfD"
     CDS             complement(8752..9873)
                     /gene="roxA"
                     /locus_tag="ACHJWA_002444"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFO4309024.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYTNGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            10122..11351
                     /locus_tag="ACHJWA_002445"
     CDS             10122..11351
                     /locus_tag="ACHJWA_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4309025.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFITQLVEQNRHTGLLLGEVKQEASVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            11716..11904
                     /locus_tag="ACHJWA_002446"
     CDS             11716..11904
                     /locus_tag="ACHJWA_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4309026.1"
                     /translation="MLSTDRFIREKECEKLTGLSRTCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            11962..12516
                     /locus_tag="ACHJWA_002447"
                     /pseudo
     CDS             11962..12516
                     /locus_tag="ACHJWA_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
     gene            12709..12906
                     /locus_tag="ACHJWA_002448"
     CDS             12709..12906
                     /locus_tag="ACHJWA_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO4309027.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARN
                     RCPSGCVAVFAARIRQEVRHD"
     gene            12899..13111
                     /locus_tag="ACHJWA_002449"
     CDS             12899..13111
                     /locus_tag="ACHJWA_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4309028.1"
                     /translation="MINLSLTDLNRIQFREKFTGQLLVNVDKGRVVCNYHLPDEAIVA
                     TRESLQEITKRAGMINTNIQGMLYAQ"
     gene            13101..13565
                     /locus_tag="ACHJWA_002450"
     CDS             13101..13565
                     /locus_tag="ACHJWA_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4309029.1"
                     /translation="MHNKTTPDAAAEAIKTLMHALIDISVIADRAHKHATSETEYAGA
                     FVPHSLAVMQFSADTALNEAKAILIADCENGGVMRDDRFNSLKQEFSGVPDDAADALS
                     SMPELIRAAFFLLSTREYKSTGLDVLNIAADYAEYVAEARYRRKFPEDVSHA"
     gene            13558..13791
                     /locus_tag="ACHJWA_002451"
     CDS             13558..13791
                     /locus_tag="ACHJWA_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4309030.1"
                     /translation="MRDIYHQTIDRAFLALSHSENMMEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            13797..14096
                     /locus_tag="ACHJWA_002452"
     CDS             13797..14096
                     /locus_tag="ACHJWA_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO4309031.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGTVMIKGVEERRVTY
                     RREGYEYDCVMPVYQFRRDFSLVQTAPHNVPTSNARARANIQKLKTMVNGFRGKK"
     gene            14093..15493
                     /locus_tag="ACHJWA_002453"
     CDS             14093..15493
                     /locus_tag="ACHJWA_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="HFO4309032.1"
                     /translation="MKSAPNLKKQPYDKMTEVIIFAGSDAWAHAKQWQEQDGRLAGDN
                     VPPVVLADDQLDELAELRIIDEGRYCVRLYKAGHIRPSNINAIAHKLAAAGVTDANYY
                     PEGMHSHMRENWREYLERVRGKEPAEEKNHQRKTTLPMSVGSTGYDTQLDYVVKGIIP
                     AVSLCSIYGASGSYKSFLAGSWACHVATGRQWGGRRVAHGAVLYVVGEGGIGVPRRVK
                     AWEVVHDEQVKNLYLVNRPIFPAAPLDVDEMVIAARQVERETGKPVRMIILDTLARCF
                     GGNDENDSRDMGAFIRGCDELKRRTGATVLVVHHSGKDETKGARGSSAFRASLDAEYR
                     IRREDAGSEALVISCTKMKDAEELKEAAYDLRVVELFTDADGELITSLVVVDDPRPPV
                     ELERIEEAGNKTENHTALWGCIRSRTQNGDKCTIPLLRDDMKKLGYEMKNFRRWLYKL
                     EKDGVIRIDGDDVAPL"
     gene            15694..15945
                     /locus_tag="ACHJWA_002454"
     CDS             15694..15945
                     /locus_tag="ACHJWA_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4309033.1"
                     /translation="MTQKRRDRTEPKYKALDMTEHTLKVAIRTIDRHTREGYAKEHPD
                     LISAFMTTAAANFATLTEREIAEAEQVTTINVKTGEVEL"
     gene            15942..16352
                     /locus_tag="ACHJWA_002455"
     CDS             15942..16352
                     /locus_tag="ACHJWA_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO4309034.1"
                     /translation="MTAQIAAYGRLVDDPQVKQTSKGTPMTLARMAVSLPCSQAQDGQ
                     ATLWLSVIAFGKQAGFLAKHQKGDVASVSGTMQVSQWTGQNGETRQGWQVIADSVISA
                     RAARPGGNRRKTTGTQGNQPPAGGDDPYGDDISF"
     gene            16363..16635
                     /locus_tag="ACHJWA_002456"
     CDS             16363..16635
                     /locus_tag="ACHJWA_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO4309035.1"
                     /translation="MVHDRIAEELEAKGFYRRAAARWGEVMQLVETDKERHHITMRRL
                     ECSRKAQRAPEPPDNFGDLKKAVDRTYAEMGIDGAGDEIWRNYQDS"
     gene            16592..16720
                     /locus_tag="ACHJWA_002457"
     CDS             16592..16720
                     /locus_tag="ACHJWA_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001301569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFO4309036.1"
                     /translation="MVLVMKYGAITRTANQTAGEIRLFLHQLKRYGALPGFRHGQA"
     gene            16762..16986
                     /locus_tag="ACHJWA_002458"
     CDS             16762..16986
                     /locus_tag="ACHJWA_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4309037.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMSINDYRAFLETGALIEKDQHG
                     FVRCALSGEMLAVNPEQIDALIEFLKEIRD"
     gene            17238..17444
                     /locus_tag="ACHJWA_002459"
     CDS             17238..17444
                     /locus_tag="ACHJWA_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4309038.1"
                     /translation="MAEEKKSGVSVYISPEIVEVLKQRHKKNYEAGVAAGLDPLMTPE
                     PSIGSLVRSYLLAALGMHKNYGGE"
     gene            17444..18499
                     /locus_tag="ACHJWA_002460"
     CDS             17444..18499
                     /locus_tag="ACHJWA_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO4309039.1"
                     /translation="MAGKATALNTNQLFMYLNRGDIADFKFSPLFTTLFFPNVATFST
                     QNIMLDTLDIEEVTMSAFCSPMVGSQVQRDKGYETSTIKPGYMKPKHEIDPTKTIMRM
                     AGEDPAQLNDPTYRRMRLITGNMRRQINAIKARVEWLAVNAVTTGKNIIEGEGIERYE
                     IDWKIPENCIIEQAKGKKWSEQDKDMHDPIYDIELYADQAGCPANVMIMGAEVWRTLR
                     SFKKFRELYDLSRGSESAAELACKNLGEVVSFKGYLGDLALIVYSGKYTDSDGTEKYF
                     LEPDLLVLGNTNNKGLVAYGAIMDQEAVRTGATQNMFYPKNWIEDGDPAIEYVQTHSA
                     PQPVPADIRKFVTVKIG"
     gene            18512..18847
                     /locus_tag="ACHJWA_002461"
     CDS             18512..18847
                     /locus_tag="ACHJWA_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO4309040.1"
                     /translation="MDTPYIELFAGSQQVSTTLVHFAADAGVIQEFTPLMLADNGEFK
                     PWDGQESGKAVYLTSYPVDTSKQKSAQCYKTGIFNIAAVNWPESVDTDAKKCAAFAGS
                     GVSVQPLAR"
     gene            18860..19273
                     /locus_tag="ACHJWA_002462"
     CDS             18860..19273
                     /locus_tag="ACHJWA_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HFO4309041.1"
                     /translation="MATNESIMTLPLASKFKVEARAMADRGLSTYEAVYQLNKLEEQD
                     KPCADAIMALHEHKDYQPLLRAMANVPCIDVGTAKSILSMTIEQERPKVAPELTAAFE
                     NFMDMHSPQAVSAGMAYDGRNQGDDGDIDRILKTI"
     gene            19479..20021
                     /locus_tag="ACHJWA_002463"
     CDS             19479..20021
                     /locus_tag="ACHJWA_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO4309042.1"
                     /translation="MKSHLMNKKNMAKSCRVSATAFDKWGVTPVERKGREAFYDVASV
                     IENRVNNAISQLINDKGEIDDDELLRVRIRLLTAQAEAQELKNDRDRGDVIDTEFCLY
                     ALSKLASQISSIMDSLPLTMQRSFPQITPAMLDRLKREVVKACNASARVADNLPQILA
                     DYLKETTGNVPEKLLQKKGE"
     gene            20277..20558
                     /locus_tag="ACHJWA_002464"
     CDS             20277..20558
                     /locus_tag="ACHJWA_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4309043.1"
                     /translation="MTEAELLGLIRRVAGISQQHDEQATQPDSVTAENYVRVVAEVMR
                     RDGIELNGVDMRNIRNRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(21159..22619)
                     /gene="phoQ"
                     /locus_tag="ACHJWA_002465"
     CDS             complement(21159..22619)
                     /gene="phoQ"
                     /locus_tag="ACHJWA_002465"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFO4309044.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMHGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITKQYEGKIVAGESMLGGARMEVIFGRQHSTPKDE"
     gene            complement(22619..23290)
                     /gene="phoP"
                     /locus_tag="ACHJWA_002466"
     CDS             complement(22619..23290)
                     /gene="phoP"
                     /locus_tag="ACHJWA_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFO4309045.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLICRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(23459..24829)
                     /gene="purB"
                     /locus_tag="ACHJWA_002467"
     CDS             complement(23459..24829)
                     /gene="purB"
                     /locus_tag="ACHJWA_002467"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFO4309046.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(24833..25474)
                     /gene="hflD"
                     /locus_tag="ACHJWA_002468"
     CDS             complement(24833..25474)
                     /gene="hflD"
                     /locus_tag="ACHJWA_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFO4309047.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLKRKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(25510..26616)
                     /gene="mnmA"
                     /locus_tag="ACHJWA_002469"
                     /gene_synonym="trmU"
     CDS             complement(25510..26616)
                     /gene="mnmA"
                     /locus_tag="ACHJWA_002469"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFO4309048.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILIVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPI"
     gene            complement(26670..27131)
                     /gene="nudJ"
                     /locus_tag="ACHJWA_002470"
     CDS             complement(26670..27131)
                     /gene="nudJ"
                     /locus_tag="ACHJWA_002470"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFO4309049.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(27141..27794)
                     /gene="rluE"
                     /locus_tag="ACHJWA_002471"
     CDS             complement(27141..27794)
                     /gene="rluE"
                     /locus_tag="ACHJWA_002471"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309634.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFO4309050.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREATE"
     gene            27966..29216
                     /gene="icd"
                     /locus_tag="ACHJWA_002472"
     CDS             27966..29216
                     /gene="icd"
                     /locus_tag="ACHJWA_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFO4309051.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYELAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            complement(29330..30472)
                     /locus_tag="ACHJWA_002473"
     CDS             complement(29330..30472)
                     /locus_tag="ACHJWA_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFO4309052.1"
                     /translation="MAASPRSHKISIPNLYCKLDKRTGKVYWQYKHPLSGRFHSLGTD
                     ENEAKQVATEANTIIAEQRTRQILSVNERLERMKGRRSDITVTEWLDKYNSIQEDRLQ
                     HNELRPNSYRQKGKPIRLFREHCGMQHLKDITALDIAEIIDAVKAEGHNRMAQVVRMV
                     LIDVFKEAQHAGHVPPGFNPAQATKQPRNRVNRQRLSLPEWQAIFDSVSRRQPYLKCG
                     MLLALVTGQRLSDICNLKFSDIWDDMLHITQEKTGSKLAIPLNLKCDALNITLREVIS
                     QCRDAVVSKYLVHYRHTTSQANRGDQVSANTLTTAFKKAREKCGIKWEQGTAPTFHEQ
                     RSLSERLYREQGLDTQKLLGHKSRKMTDRYNDDRGKDWIIVDIKTA"
     gene            complement(30462..30698)
                     /locus_tag="ACHJWA_002474"
     CDS             complement(30462..30698)
                     /locus_tag="ACHJWA_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO4309053.1"
                     /translation="MSRLITLRDWAKEEFGDLAPSERVLKKYAQGKMMAPPAIKVGRY
                     WMIDRNSRFVGTLAEPQLPINANPKLQRIIADGC"
     gene            30838..31626
                     /locus_tag="ACHJWA_002475"
                     /pseudo
     CDS             30838..31626
                     /locus_tag="ACHJWA_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247981.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
     gene            31623..>31921
                     /locus_tag="ACHJWA_002476"
     CDS             31623..>31921
                     /locus_tag="ACHJWA_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO4309054.1"
                     /translation="MKNIAAQMVNFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVNAGMRVARRQNRPFL"
BASE COUNT         8387 a         7435 c         8015 g         8084 t
ORIGIN      
        1 attagaaatg aaacaggatc gtggggggaa tagatatagt gcagtagatc ttaactcttc
       61 taagtatggg tattatgaga aagttggcgg tggtttttat agatatatag aatcctttaa
      121 ccccatatct tcagagacac caaataaaat agtctacaag ggggaatcag tagatttaac
      181 taaggagcca aattcggaat tatattcagg taggtattct ataaataaca aacaggttaa
      241 tgtttatttc tttcgtgacg ctgatggtac attttataaa tcagaaggtc ttcatggtgg
      301 gggagttatt agatacatag ataaaccgta ttctcagtta agagaaggag atattgggta
      361 tgatgaggat ttgttggata tatacgatga ttctccggtg cttgaagaca cgttgcctgc
      421 tttatcttct gaaatagtac caactccaga acatagtatt aaacaaattt attcgaaaat
      481 taaggagggg cacatagaac tgtccgattc agacatcata ttgtgtcgcg gcacaaccgg
      541 tattcaagct gaaaatatcg ttgaatataa aactgctgga gggcttcctg attcaaatcc
      601 aaatgtaaaa gcaccagatg aatatatggc acaacagcag gtacgtattg gaagaatatt
      661 gcctgaatac acatcggatc ttagcgttgc tgatcggttt agtcgtgagc attatctaat
      721 agttgttaaa gtaaaggcaa aatatatcac acgaggaagt gttacagaga gtggttgggt
      781 tatagataag accgcacctg ttgaaccact tgcgataatt gatagaactt ttggtatgaa
      841 ggaaaatatc tcaatggtaa atgcatcgaa atagtttttt tacaatctat gtcctgcctc
      901 ctctggtaaa aacgatgcta catcttggag atgttgcacg gcagtacggt gttgaccgat
      961 atgtagtgaa gtacacatcg gtcaacgaat taccattgtc agcaatatca tcctgataaa
     1021 ctcagtactc gtgagccgct tgatgacggg cggaatagcc caaaaggtaa acgatccgca
     1081 tggcagtccg tcggaaagct gctgcttcat acgaccgctt aaaccgtcag ttagtgtcag
     1141 tatcgctgaa gatcagcttc ttttgctgat ttactctgtt tttacctgcc ctgatgaaat
     1201 actctttaat cataacattg attatatcga aaatatattt tttgctatca ttaagaatat
     1261 ttatatgtgg aaccggagta ttttggacgg tagtctttcc actctggtgt atctaatatt
     1321 ctttctgctg gtatctcttt gccatgcaga taaaatttta tgtttttcat tatttggctg
     1381 ttgtctttat tacgataaat ccatctggat ttgcccctat atttccagac atctgttatc
     1441 acttaaccca ttacaagccc gctgccgcag atattcccgt ggcgagcgat aacccagcgc
     1501 actatgcgga tgccattcgt tataatgctc gaacgcctct gcaaggttct ttgctgccgt
     1561 taacccgtct ggtttgggca tgatactgat gtagtcacgc tttatcgttt tcacgaagtt
     1621 ctctgctatt ccgttactct ccggactccg caccgccgtg ttcttcggtt caagtcccaa
     1681 catccgggcg aactggcgtg tttcattagc ccggtagcat gaaccattat ccgtcagcca
     1741 ctccactgga gacgacggaa gatcgttgcc gaagcggcgt tccaccgctc ccagcatgac
     1801 gtcctgtact gtttcactgt tgaagccgcc ggtagtcacc gcccagtgca gtgcctcacg
     1861 atcacagcag tccagcgcga acgtgacacg cagtctctct ccgttatcac agcagaactc
     1921 gaacccgtca gagcaccatc gctgattgct ttctttcacg gccactctgc ctgtatgtgc
     1981 ccgtttcgat ggcggtacag taggttttcg ctcaagcaac agcgcattct ggcgcatgat
     2041 ccggtaaaca cgtttggcat tgatcgcagg cataccatca agttctgcct gtctgcgaag
     2101 cagcgcccat acccgacgat aaccatacgt gggcagctct ccgataacat ggtgtatacg
     2161 gagaagcaca tccgtatcat cagtgtgacg actgcggcgg ccatccatcc agtcatcggt
     2221 tcgtctgaga atgacgtaca actgcgcacg cgacacccgg agacaacggc tgactaagct
     2281 tactccccat ccccgggcaa taagggcgcg tgcgctatcc acttttttgc ccgtccatat
     2341 tcaacggctt ctttgaggag ttcattttcc atcgttttct tgccgagcag gcgctggagt
     2401 tctttaatct gcttcatggc ggcagcaagt tcagaggcag gaacaacctg ttctccggcg
     2461 gcgacagcag taagacttcc ttcctagtat tgcttacgcc agagaaataa ctggctggct
     2521 gctacaccat gttgccgggc aacgagggag accgtcatcc ccggttcaaa gctctgctga
     2581 acaattgcga tcttttcctg tgtggtacgc cgtctgcgtt tctccggccc taagacatca
     2641 atcatctgtt ctccaatgac tagtctaaaa actagtatta agactatcac ttatttaagt
     2701 gatactggtt gtctggagat tcagggggcc agtctagtca tctacacaac caacgccatc
     2761 gagtcgctga acagcgtaat ccggcatgcc atcaaaaaac gcaaggtgtt cccgacggat
     2821 gactcagtga aaaaggtcgt gtggctggca atccaggcgg cttcacagaa atggacgatg
     2881 ccactgcggg actggcgtat ggcaatgagc cgctttatta tcgagttcgg tgaccgcctg
     2941 gacggtcact tctgagaaaa ggcatttaca cagaatcgtg tacagggtca aatattttaa
     3001 ttttactaca ttgcatgatt acaatcattt ttgtgacttt attgagttta aacacaaaaa
     3061 tattattatg aacacaagtc agtacacagg aagttcatgg taaaaccacc tcctgtggag
     3121 gtggttgctt gcagtcaggt gtttcgggtc tgtggctttt tgtgttctct ggtaagtggt
     3181 tgctattctt ttgtgttgtg tatctctgtt tctatagttt tagatagcca tgtcatctca
     3241 gtaactgaga gttttagttt tggggttctt tctcctgcgc cgggtgggcg catgtcaaaa
     3301 ctacaggctt ttttttggta cactgtatag ctatctacat tacatgttgt tgatgtaagt
     3361 atgcagttct ttgacgctgg cttgtctttt atgggaccgg atatttccgg gtaatgtact
     3421 tgtgaacttg aaaatctgtt tttgattgaa aatggcattg ttttctcctt tgctccatga
     3481 aaagtgtttt tatgctattc cgttataagt tggtatcatt aagaatattt atatgtggaa
     3541 ccagagtatt ttggacgata gtctttccat tctggtgtat ctaatattct ttctgctggt
     3601 atctctttgc catgtagata aaattttatg tttttcatta tttggttgtt gtctttatta
     3661 cggtaaatcc atcgtaattc gctggcggta atactctggc catagccctc tgacttgtta
     3721 gctatgagtt tgagtgagtt gacaagctca tccgcacaaa aaataaccgt tttattgcgt
     3781 gttaatgtct gaaactctag gccggccttg cttgtttttg cccataattt gtcgcgattg
     3841 tatacggaaa ggtcggccat ggtaacatta taattattgt gattctttaa gtgttgttta
     3901 aattttattc ctctttcgtg ttcatttaat gctcttttga taagtggatc ttgcttatat
     3961 ttgcgaggtg ttctggggtc ccatactgcg tttgttaacg tattctgagt tataatatct
     4021 tttttggaaa agtcgttaac tagtttaatg ctgttgacaa gtttttctct gggtgaagat
     4081 agtccataga caatgtctcc ctcgcgaaag tgtaaggcca catccagagc gttgccctga
     4141 aaggtgatgt tttttgcttt aactacatct ggagagagta gtacattttg taaatcaaga
     4201 tccagcccat caggcatccc ttcaacaccg atgttccacg tggttttctg ctccgcatga
     4261 atggttaccg atgtcacact ctccggaagt ttctccggca gtataatatt gctacatcca
     4321 gtcagcgata gcgtcttcag tgacggtgat atcaggttgt caattctggc ctgattctcc
     4381 gggttatagc tattgatgct gagagatgat aacccgtttg gaacattttt tatgctgtct
     4441 gtggcgctac cttttatttc aaggtagcgg acactctctg gcactccaga tatatagcag
     4501 tggcaaaccg tcaggcactc gatgccttca gatatgaaat gtataaatgg tgaatcagtt
     4561 aattcaatat gtagattttt tacggttgat ggcatgtctg tgatttgtaa tttttcacag
     4621 ttaatgacgg agaggttttg tgtattaacc ggcaatcctg taagggttgt caggttgttg
     4681 cagttttcga ttgtaactga tgttatttcg tttggtaact gtggcagata ctcaatatca
     4741 caatctttga tatataaacg accactggcc cgcgcttctt ctatctgaat tggtgtgatt
     4801 tcgcttttga tgtctgctga tgtttcagcg gaataggagc agaatgtccc ggtcggtcgt
     4861 aattgcgatg taggaagcat tgataaattc catttttaga aataaataac agagtaagtt
     4921 ctatccatac tactaatcaa caagctaatt ttaacaacca gcaaagccaa ggtgtcaata
     4981 ttagaaaaga gagttatatc tcaaaaccaa cagactgttg attttaactt ttgcgaatac
     5041 tttctccagt ccgtcatgca tggattagga ttgctcattt ttataccatt tgttgttttt
     5101 ttatagcgat ttgctaaaag ggagaagaaa aaacgttctc caaaattaga ttgcaactgt
     5161 ttgattttat ggatagaggc atgctgttta ctagacagta aaaagcatgc caaatttcct
     5221 attaaccttt tgaatataaa gcactaatta tctatttctg ttgccttctt attcagcaga
     5281 cgagaagcgc gccagtaaac cagcaacatc aggcccatta cgatagtcag cgtaatgctg
     5341 gtggctgcac caaacggcca gtcacgaata ttgaggaact gtaccttgat gacgttaccg
     5401 atcagcaggt ttttcgcacc gcccatcagg tcggacacat agaacaggcc catcgctggc
     5461 agcatcacca gcagacatcc ggcaataatc cccggcatcg tcagtggaat aatgatgcgg
     5521 ataaaagtct gtaacttgct ggcaccgaga tcgcgcgccg cctcaagcaa cggcttatcc
     5581 agtttttcga tactggagta cagcggcatc accataaacg gtagcagaat gtaaaccaga
     5641 ccgataatca ccgcactggg cgtgaacatg atacgaattg gtgtgtcgat aacgcccagc
     5701 cagagcaaaa actcgttgag atagcctttg gtgctgagga aaattttcag cccgtagatg
     5761 cgaatcagtg agttggtcca gaagggaaca atcagcagaa acagcagcag cggacgcacc
     5821 ttgtgtggca gcttcgccag aaaccaggca aacggatagc ccagcaccag gcaggcgagg
     5881 gtggcgatca gcgccatatt cagcgagtgc aatagcactt caaaatagag cggatcgagc
     5941 agacgtgtgt agttatccag cgtaaagacc attttgacaa aactggcgtc gtcgcgggcc
     6001 aaaaagctgg tgccaatgat catcaggttg ggcagaaaga caaacaacac aagccaaccg
     6061 acaatagtga caatcactac attctggaac ttacttgtgt tcttcatcag ccagtacgac
     6121 ctcccagctt tctacccaat taatggccat tttttggtcg agagagtggt caaagtcagg
     6181 atcgtcttca ttgaagaatt cgctgaccat caccatcttg ccattttcca gttcaacaac
     6241 cgactccagc gtcatgcctt tgtagttacg ctcgcgaacg taaccaatca gcccttcagc
     6301 gtggttgtcg tcgttaatct cttcaacacg taagtcttcc gggcgcagca gaacatgcag
     6361 tttttgcccc ggttcaacgg cgaagttaac gtagatatta cattcgcggc cttcaacgtt
     6421 ggcgcgtacg cgctgctcgt cgagacgttc gatgacagtg gcgttaaaca tattgatttc
     6481 gccaatgaac ccggcaacaa acaggttttt cggctcttcg tagatttcac gcggcgtgcc
     6541 gtcttgctca atgcgaccat cgcgcatcac cacaatcctg tctgacatgg tgagtgcttc
     6601 ttcctgatcg tgagtcacga agacgaatgt aatgccaagc ttacgctgta acgctttcag
     6661 ctcgttctgc atttgcttac gcagtttgta atccagcgct gagagcgact catccagcaa
     6721 caacagacga ggcttgttaa ccaccgcgcg agcaatggcg acgcgttgct gttgaccacc
     6781 agagagctga tgcggtttac gttgagcgaa ggtttccagc tgcaccatcc gcagggcttc
     6841 catcacgcgg ggcgtaattt cagcagcggg ggttttttgc atgcgcaacc caaaggccac
     6901 attttcgaac acggtcatgt gggggaaaag tgcgtagctt tggaaaacag tgttcacata
     6961 gcggttttcc gccggaacgt gggtgatgtc ctcgttatcc aacatgatgc gtccggaatc
     7021 aacagtttcc agacctgcaa tcaggcgaag aacggttgtt ttaccgcagc cagaagggcc
     7081 aagcagcgtg aggaactcgc cattgttgat agtcagatcc agctggggaa tgacctcttt
     7141 accatcaaag catttgcgaa ttcccgccaa ttgcaccagc ggtgaaagcg aactcggttg
     7201 tttattcaat tttttactct gtcccatgta aacgcaacgg atggcttacc gatgcggggt
     7261 ttgtggttaa ccaccttggt gactcttaat gagggcggta attctacggc aaaccgcttg
     7321 aatcgccaat ctttgttgtg aattactggc ttagctttat attcattaag gtaatgctga
     7381 taaatattcc cgcttgcagg ggtaaaagtg acctgacgca atatttgtct tttcttgctt
     7441 cttaataatg ttgtcacaaa aagtgagggt gactacatgg ataaactact tgagcgattt
     7501 ttgaactacg tgtctctgga tacccaatca aaagcagggg tgagacaggt tcccagcacg
     7561 gaaggccaat ggaagttatt gcatctgctg aaagagcagc tcgaagagat ggggcttatc
     7621 aatgtgacct taagtgagaa gggcactttg atggcgacgt taccggctaa cgtccctggc
     7681 gatatcccgg cgattggctt tatttctcat gtggatacct caccggattg cagcggcaaa
     7741 aatgtgaatc cgcaaattgt tgaaaactat cgcggtggcg atattgcgct gggtatcggc
     7801 gatgaagttt tatcaccggt tatgttcccg gtgctgcatc agctactggg tcagacgctg
     7861 attaccaccg atggtaaaac cttgttaggt gccgatgaca aagcaggtat tgcagaaatc
     7921 atgaccgcgc tggcggtatt gcaacagaaa aacattccgc atggtgatat tcgcgtcgcc
     7981 tttaccccgg atgaagaagt gggcaaaggg gcgaaacatt ttgatgttga tgccttcgat
     8041 gcccgctggg cttacactgt tgatggtggt ggcgtaggcg aactggagtt tgaaaacttc
     8101 aacgccgcat cggtcaatat caaaattgtc ggtaacaatg ttcatccggg cacggcgaaa
     8161 ggagtgatgg taaatgcgct gtcgctggcg gcacgtattc atgcggaagt tccggcggat
     8221 gaaagcccgg aaatgacaga aggctatgaa ggtttctatc acctggcgag catgaaaggc
     8281 accgttgaac gggccgatat gcactacatc atccgtgatt tcgaccgtaa acagtttgaa
     8341 gcgcgtaaac gtaaaatgat ggagatcgcc aaaaaagtgg gcaaagggtt acatcctgat
     8401 tgctacattg aattggtgat tgaagacagt tactacaata tgcgcgagaa agtggttgag
     8461 catccgcata ttctcgatat cgcccagcag gcgatgcgtg actgcgatat tgaaccggaa
     8521 ctgaaaccga tccgcggcgg taccgacggc gcgcagttgt cgtttatggg attaccgtgc
     8581 ccgaacctgt tcactggcgg ttacaactat catggtaagc atgagtttgt gactctggaa
     8641 ggtatggaaa aagcggtgca ggtgatcgtc cgtattgccg agttaacggc gcaacggaag
     8701 taagcgaaaa gggatgcggc atgtgatgcc gcatccggct taaatccaaa cttacccttc
     8761 gaagaaccaa tacccgctat tgaccagcgc cgcgagcatc gcgaggaatg acggatcttc
     8821 cagcgcatcg ccaaaattct ccgcagtcag cgcaatgttg ctggcgagtg catccagtgc
     8881 cggacggtgc ggggaatcga tcttctcacc attggtatac acgtcgtcgc caatgcgcaa
     8941 tacgcgcaga ccacccaggc gcaccagcac atcaccttgt ttcagcgcat cgtagatttc
     9001 atctggctga taaggcggct ccggcggcgc gatatccagt tcatgacgtg actgggatat
     9061 aaactcgcca aaccattgct taaagtgttc cggctggttg atcaattcga gcatcatctc
     9121 acgcagttta tccatctctt gcggcagaac gtccgcagga tgagcgcgag gtggaacatc
     9181 aggatcgctg tagtagttgc cgcccagttc gcgttgcagc acataatcgg caaatccgct
     9241 aattaattcc cgcgtatttg gcgcgcgaaa acccacggaa tagttcatcg cattttccag
     9301 cgcgtagcct tcatgcggga atcctggcgg aatataaaga atatcgccag gctccagctc
     9361 ttcatcgatg atggcttcga acggatcgac ctgtaacaga tccggatgtg ggcagtgctg
     9421 tttcatttgc agcttctcgc ccactcgcca gcgacgacgt ccggtaccct gaatgataaa
     9481 cacgtcgtac tgatcgagat gcgggccgac gccgccgcca ggtacagaaa aagagatcat
     9541 cagatcatca atacgccagt ccggtagttc acggaacggt cgcatcagcg cggcggtcgg
     9601 ctcatgccag tggttcactg cttgcactaa caatgaccag ttagtttcac cgagatgatc
     9661 gtagctttcg aacggaccgt ggctgacctg ccattttcca tcctggtgac tgaccagtcg
     9721 actgtcgact tcgctttcca tcgccagacc cgccaactcg tctggagaga tcgggtcaat
     9781 aaaattatta aagccgcgtt ttaacaccac cgggcgtttc tgccagtgac gttcaagaaa
     9841 atcgggccag ttaagagtga gttggtattc catgttaagc atccacaggc tggtatctgc
     9901 aaccgattat aacggatgct taacgtaatg cgtgaagtat ggacatattt tatcaattag
     9961 tgttttttgc cgtttcataa tgtttcagat tgttcattat atttttgatt ataaacagat
    10021 ttgtgatttt ccttgttcca gcttgtttca cgttgtagcc ctttgtgcaa cgatcggtgt
    10081 agttaatggt gtagttaatt gtgatgaccg gaatcaaaat tatgagcaga gcacttaaca
    10141 aactgagcga tacacagctg aggaaaatca acggcacacc cgcccaaaaa acagcctttc
    10201 ttaatgacgg tggaaacctg agcgtcaggc attcaaccag tggcctttta acctggtatt
    10261 tcacttacag ggccggaacg ggaagggggg caccaccgga acgcattaag ctgggaaatt
    10321 atcctgatct gagcctgaaa tcagccaggg aaaaagccgc ccagtgtcgc gcatggctgg
    10381 cagaggggaa aaatccacgt catgagctta attacaccgt acaggaagcg ttaaagccgg
    10441 taacggttgg cgatgcgctc acctactggc ttgagtcgta cgcaaaggaa aaccgcgtgg
    10501 attatgccgc cctgaaaaag cgccttaata atcacgtaat acagcacatt ggtgctatgc
    10561 cgctggataa atgcgagcta cggcactggc tggcctgttt tgaccaggtg gcaaagcgaa
    10621 cgcctgttac tgccggattc ttgctacaga cgtgcaaaca ggcgcttaag ttctgccgga
    10681 ggcggcgcta tgcaatcagc aacgttcttg atgatatgag tgtggcggac gttgggaaaa
    10741 aaccggatat aagcgagcgt gtcttaagca ccaaagaact gggcgaatta ttgcaggcac
    10801 tggacaaaaa aatattctcc ccctactaca tcgcgttaat ccgcctcctg attgtgttcg
    10861 gatgccggac ggtagaactg aggttatcgg agatcagcga gtgggatttt accgaaatgc
    10921 tctggaccgt tccgaaggag cacagcaaaa cgaaggtagc catattccgg cctataccgg
    10981 aagcaatact gccgttcatc acgcagctgg tggagcagaa caggcacacg ggcttattgc
    11041 tgggggaagt gaaacaggag gccagcgtat cgcagtatgg aagattagcg cacaggaggc
    11101 ttaatcaccc tcactggtca ctgcatgaca tccggcgcac ctttacaacc atgctgaacg
    11161 atttaggcgt tgatccgcat gtcgtggagc agcttacagg ccaccagatg ccaggaatgc
    11221 agcgagttta taatcattcc cgttatctgg atgcgaaacg caatgcgctg gatatgtgga
    11281 cggagcggtt agggatactg gcgggaacac atgaaaacgt aaccacgcta ccagtagcca
    11341 gaagaaaata atttttttcg tgttttttca gtatgcgcat actggatatg cgaacagata
    11401 caacgtgcaa caatgagcaa caatgtggaa caactacgaa caagaggcga aaaagcgtac
    11461 ggatttataa gtagctgatt tttaatgtgt tactggtttt ttatacacta gcgaatcact
    11521 ctttaaagag cgagaaaaaa ggcatggaac aatattttat gcctttgtat taacatgaat
    11581 ttaaaagatt ttattcttac tttttcattt tctgtaaatc atacatcctc agtatttgcc
    11641 attcatcact ctagcgggaa caatacgaca caataagaca cctgatgact ctttaagaaa
    11701 cgaaaggggg caatagtgtt aagcactgat cggtttatac gtgaaaaaga atgcgaaaaa
    11761 ctaaccggcc ttagccgtac gtgtcgctac cgcctggaaa aggccggaca attcccatca
    11821 cgtcgtaaac ttggcggtcg ttccgttggc tggtctttat ccgaggttct ggcctggaag
    11881 gatagctgca aggcagttca ttaatcacgc tggcggcaca cagccgccac acatcaatca
    11941 tctgaacaca gagctataac catgaagatt gaatatacgc cagaacgtgg gcggggattc
    12001 gttcgccctg gtgagactgg aaaaccacaa aattggggtt tttcaggcat aaaaaaagcg
    12061 gccccgaaat ggagccgcct ttctgaacag ataacccgct gcgccttatg tgtatatgat
    12121 cccaaacata agcacgggga tgatagccgc tatcaggctg gtgggcaatg ctatcagtct
    12181 ggttcagttc gttgccatac ctgcaatggg cgcgtttccc tgtactcttt aaggaattgc
    12241 tcaagggcaa aagcacatgg cgcgaatctt tctgattcat gctctatctt tctgcgccgt
    12301 cttttccgtg ccggtgataa tgttttggtc aattctttat cggtcattgt gttgtcctgc
    12361 atagcaatgc gccgtaatac ctcacaccac ggcgctgata gtttttatcc tttgggttct
    12421 atgccgcgtt gctgtaattc tttgcggaga acgcgtttaa tccaagtggc taaagatgtg
    12481 tcgccatcgg ccttagcagc ttcttctagc tgcgctctga agtcttccgg taatcgcatt
    12541 tggtatggag gcgaacgttt ttctaatggt gttgacatgg taattaccct ttattgctat
    12601 acctacatgg taattaccat tgtacttgcc attacacaaa aatcaacgcc ccgcagtgct
    12661 gggaacacat acggggcgtc taaccaacaa cgtaaactag gagccgttat ggttgccgta
    12721 aatcatatac cacaccttgt acacacacaa acggcctttg tgtggcgttt tctggcactg
    12781 agtgccggag aatctcaaat catccacgta accgcctgga cggaacgcga agcgcgtaac
    12841 cgttgcccgt ccggttgtgt tgctgtattt gccgcccgta ttcgtcagga ggtgcgccat
    12901 gattaatctg tcacttactg accttaaccg cattcagttt cgtgagaaat tcacagggca
    12961 gctactggtc aatgtggata aaggtcgcgt ggtgtgtaat taccacttac cagatgaagc
    13021 aattgtcgcc acaagggaat cattacagga aatcacaaaa cgtgccggaa tgattaacac
    13081 gaatattcag gggatgcttt atgcacaata aaaccacacc ggacgcagcc gccgaagcca
    13141 tcaaaacgct gatgcacgcg ctgattgata tttctgttat cgcggacagg gcacataagc
    13201 acgccaccag cgaaacagaa tatgccggtg ctttcgttcc tcactcactg gctgtcatgc
    13261 aatttagtgc tgatacggcg ctgaatgagg ccaaagctat cctgattgct gattgtgaaa
    13321 atgggggggt tatgcgtgat gatcgtttta attccctgaa acaggaattt tccggcgttc
    13381 ctgatgatgc ggctgatgcg ctttcgtcaa tgccagaact tattagagcg gcttttttct
    13441 tactttccac gagagaatat aaatcaacgg ggcttgatgt actgaatatc gccgccgatt
    13501 atgcggaata tgtggcagag gcgcgttata gaagaaaatt tcctgaggat gtaagccatg
    13561 cgtgatattt accaccaaac aatagaccgc gcatttcttg cactttctca cagtgaaaac
    13621 atgatggaaa tattgcgcat atggcttgaa acacttggcg acaatgaacg cgacaaacaa
    13681 aaatcaagaa ttgccacggc attaataacg cttcttgagc ctgtaataat ggaactgcaa
    13741 gaaatagatc tattgcacga cagatataaa gaacagcaca ccggagaata aaaataatga
    13801 aacttaaata ttctggctta actgccagtg gcaacactca ccctaaattt acgcgcggtg
    13861 atatttaccg cgaccagtac ggcggcacgg taatgattaa gggcgtggaa gaacggcgcg
    13921 taacctaccg ccgtgaaggt tacgaatatg attgcgtgat gcctgtttat cagttccggc
    13981 gtgatttttc tctggtacag accgcgccgc ataacgtgcc caccagcaac gccagggcac
    14041 gggcaaacat ccagaagctg aaaaccatgg ttaacggatt caggggcaag aaatgaaaag
    14101 cgcaccgaac ttaaaaaaac agccttacga caagatgacc gaagtcatta tttttgcggg
    14161 tagtgatgcc tgggcacatg cgaaacagtg gcaggaacag gacgggcgac tggctggcga
    14221 taatgtgcct cccgttgtgc tggctgatga tcaactggat gaactggcag aactgagaat
    14281 catcgacgag gggcgctatt gtgtccggct gtacaaggca ggccacatca ggccatcaaa
    14341 tattaatgcc attgcgcaca agctggcggc ggcgggtgta actgatgcga attattaccc
    14401 cgaagggatg cacagccata tgcgggagaa ctggcgcgaa tacctggaac gggtgcgcgg
    14461 gaaagagccg gcggaagaaa aaaaccacca gcgaaaaacc acgctaccga tgagcgttgg
    14521 atctaccgga tacgacacgc aactggatta cgtggttaag gggattattc cggcggtatc
    14581 gctatgcagc atatacgggg ctagcgggtc ctataaatca ttccttgccg gatcgtgggc
    14641 gtgccatgtt gctactggtc gccagtgggg aggccgcagg gttgcacatg gtgcggttct
    14701 ctatgtggtt ggtgaaggcg gtataggtgt tccgcgtcgt gtaaaagcct gggaggttgt
    14761 gcacgatgag caggtgaaaa atctgtatct ggtaaaccgc cccatctttc cggctgcccc
    14821 gcttgatgtt gatgaaatgg ttatcgctgc ccgtcaggtg gagcgggaaa cgggtaaacc
    14881 tgtacgcatg attattctgg atacgctggc gcgttgcttt ggtgggaatg atgaaaatga
    14941 ttcccgtgat atgggggcgt ttatccgtgg ttgtgacgaa ctgaaacgac gcacaggggc
    15001 cacggtgctg gtggttcacc attccggcaa ggatgagacg aaaggcgcgc gcggttccag
    15061 tgcatttcgt gcttcgctgg atgctgaata ccggatacgc agggaggacg caggaagcga
    15121 agcgctggtt atctcatgca ccaaaatgaa ggacgcggag gaactcaaag aagccgcata
    15181 tgacttacgc gtggtggagc tttttaccga cgctgacggt gaattaatca cgtcgctggt
    15241 ggtggtggat gatccgcgcc ctcctgttga actggagcgc atcgaggagg cagggaacaa
    15301 gacggaaaac cataccgcgc tatgggggtg catccgttca cgcacacaga acggcgacaa
    15361 gtgcacgatc ccgctgttac gtgatgacat gaaaaagctg gggtatgaaa tgaaaaactt
    15421 ccggcgctgg ctgtacaagc tggaaaaaga tggggttatt cgtatcgatg gggatgatgt
    15481 agcgccgcta taaaagtgag gagcaaaagc gagggggata gaaagagggc caaaattagc
    15541 ccgctctccc tcacttttcg acctgtatac atcctcaaaa gtgaggggta aaaaaatact
    15601 tatgaaacac acacatagaa aaaccgaaaa tcccaactgc gacgaagtga gacgcttgaa
    15661 aaagtgaggc gaaaaagtga gaggttgcga gaaatgaccc aaaaacgcag agacagaaca
    15721 gagccaaaat ataaagcgtt agacatgact gagcacacct taaaggtggc aatcagaacg
    15781 atagaccgcc acacgcggga aggatacgcg aaggaacatc ccgacctgat aagcgcattc
    15841 atgaccacgg cggcggcaaa ctttgccacg ctgacagaac gggagattgc cgaagcggaa
    15901 caggtaacaa ccatcaacgt taaaaccgga gaggtggaat tatgacggca cagatagccg
    15961 cttacgggcg gctggtggac gacccgcagg taaaacagac cagcaagggc acaccgatga
    16021 cgctggcgcg tatggcggta tctttgccat gcagccaggc acaggacgga caggcgacgt
    16081 tatggttatc ggtcatcgca tttggcaaac aggccggctt cctggctaaa catcaaaaag
    16141 gcgatgttgc cagcgtatcc ggcacgatgc aggtaagcca gtggaccgga cagaacggag
    16201 aaacgcggca gggctggcag gttatcgcag acagcgtaat cagtgcccgc gcggcacgtc
    16261 caggcgggaa cagacgcaaa accacaggca cacagggtaa tcagccacca gcgggaggcg
    16321 atgaccctta cggtgatgat atttcgttct gagggggtga cgatggtaca tgaccgcata
    16381 gcggaggaac tggaggcgaa aggcttttac cggagggcgg cggcgcgatg gggtgaagtc
    16441 atgcagctgg tggagacaga caaggaacgg catcacatca cgatgcgacg gctggaatgt
    16501 tcaaggaagg cacagagggc accggagccg ccggataatt tcggagacct gaaaaaggca
    16561 gtcgatcgca cttatgccga aatgggtata gatggtgctg gtgatgaaat atggcgcaat
    16621 taccaggaca gctaatcaaa cagccggaga aatccggctt tttttgcacc agttgaaacg
    16681 gtatggcgca ttaccgggtt ttcgtcacgg tcaggcatag ttactatctg aaacaaacag
    16741 acacaacaga ggaaaaaaac aatgccgatg aaatttgatg agatattaaa acagcgtgat
    16801 aaataccatg ctgacaacat ggagacgatg agcatcaatg attaccgcgc attcctggag
    16861 acgggcgcac tgattgaaaa ggatcagcat ggttttgtga gatgtgctct atccggtgaa
    16921 atgctggcgg taaatcctga acagatagat gcattgatag aatttctgaa agagatcaga
    16981 gactgagcca gcacacagca cacatagccg gagcaatccg gcttttttgc gcccaaaaaa
    17041 agcccgataa ggtcagaggg ttcttatcgg gcttttgcat atgaggtttt tggatgcact
    17101 ggcgttcgtg atcgggataa tcatttcata atttgcaaca taactcaata tcattgcata
    17161 aaatgcaatt ctgattataa tcaggactgg ataaacatcc agttatgatt tttttagtcg
    17221 aagaggaatt tcttactatg gctgaagaga aaaaaagcgg tgtttcggtg tacataagcc
    17281 ccgaaatcgt ggaggtgctc aagcagcgcc acaaaaaaaa ctatgaagct ggcgttgcgg
    17341 ctggactgga tccgctgatg acgccggagc cgtcgatagg ttcacttgta cgctcttatt
    17401 tacttgcggc gcttgggatg cataaaaatt atgggggtga ataatggcag gcaaagcaac
    17461 ggcacttaac actaaccagc ttttcatgta cctgaatcgc ggggatattg cggattttaa
    17521 attcagccct ctgtttacca cgctgttttt cccgaacgtg gcgacattca gcacgcaaaa
    17581 catcatgctg gataccctgg acattgaaga agtcaccatg tcggcgtttt gttcgcctat
    17641 ggtgggtagc caggttcagc gcgataaagg gtacgaaacc agcacaatca aacctggcta
    17701 catgaagcca aagcacgaaa tcgatccaac gaaaaccatc atgcgcatgg ctggagaaga
    17761 tccggcacag cttaacgacc ctacctatcg ccgtatgcgc ctgattactg gcaacatgcg
    17821 ccgccagata aacgccatta aggcacgcgt ggaatggctg gcggtgaatg cggtaacgac
    17881 cggaaaaaac atcattgagg gcgaaggcat agaacgctat gaaatcgact ggaaaatacc
    17941 ggaaaactgc atcatagagc aggccaaggg taaaaaatgg tccgagcagg ataaagacat
    18001 gcacgaccca atctatgaca tcgagcttta tgctgatcag gctggttgcc ccgcaaacgt
    18061 catgattatg ggcgctgagg tatggcgcac attacgcagc tttaaaaaat tccgtgagct
    18121 gtacgatctt tcccgtggtt cagaatccgc cgccgaactg gcctgtaaaa acctgggcga
    18181 agtggtgagc tttaaaggct atcttggtga tctggccctt atcgtctatt ccggcaaata
    18241 cactgacagc gatggtaccg aaaaatattt ccttgagcct gatttgctgg tcctgggtaa
    18301 caccaacaat aaagggctgg tggcctatgg tgcgataatg gatcaggaag cggtaagaac
    18361 gggcgcaaca caaaacatgt tttacccgaa aaactggatt gaggacggcg atccggcgat
    18421 tgagtacgtg cagacacaca gtgcaccgca gccggtaccg gcagatattc gcaaatttgt
    18481 taccgtcaaa attggttaac ggggggattc tatggacact ccatacattg agttatttgc
    18541 aggcagtcag caggtatcca cgacgttggt acattttgcc gctgatgctg gcgttattca
    18601 ggaatttacc ccgctgatgc tggcagacaa tggcgagttt aagccgtggg atggtcagga
    18661 atctggcaag gctgtttatc tgacctcgta ccctgtggac acgtcaaagc agaaatcagc
    18721 acagtgttac aagacgggga tattcaatat tgccgccgtt aactggcctg agagcgtcga
    18781 caccgatgcg aaaaaatgcg ccgcctttgc gggttccggc gtatccgttc agccgctggc
    18841 ccgataagca gggggaacga tggcaacgaa tgaaagcatc atgacgctac cgctggcgag
    18901 taaatttaaa gtcgaagcgc gggcaatggc tgacagaggt ttatcaacct atgaggccgt
    18961 atatcaacta aacaaactgg aagagcagga caagccgtgc gctgatgcga ttatggcgct
    19021 tcatgagcat aaagactatc agccgctgtt gcgtgcaatg gcaaacgtgc cttgtatcga
    19081 tgttggtacg gctaaaagca tccttagcat gaccatagag caggaacgtc cgaaggttgc
    19141 gccggaactt accgcagcct ttgaaaactt tatggacatg cacagcccgc aagccgtatc
    19201 agctggcatg gcatacgatg gcagaaacca gggcgatgac ggcgacatcg atcgcatact
    19261 gaaaaccatc tgagacaagg ccggagaaat ccggcttttt tacgggtcct tcctggaatt
    19321 atggcccgtt acggggcggc gacctcgcgg attttcgcta tttatgagcc ttttcagggt
    19381 ggtggtggtg gttttgttgt ttgatctatc tttatgaatg aaaagggaaa gatgcaagca
    19441 atacaccaac ctgaagcagt aattaagttg gtgtattaat gaaatctcat ctgatgaaca
    19501 aaaaaaacat ggcgaaaagc tgccgtgtaa gtgcgacagc gttcgacaag tggggagtaa
    19561 ctcccgttga gcgtaaaggc cgtgaggcgt tttatgatgt tgccagcgtg atagaaaata
    19621 gggttaacaa tgcaattagc cagcttataa acgacaaagg cgagattgac gatgatgaac
    19681 ttttacgagt caggatcaga ttactgacag cacaggcaga ggcgcaggaa cttaaaaacg
    19741 atcgcgatcg cggtgacgtg attgatactg agttttgcct gtacgcgctt tcaaagctgg
    19801 cgagtcagat ttcatcaatc atggacagcc tcccgcttac tatgcaaagg agcttcccac
    19861 agattacccc cgccatgctg gacaggctta aaagggaagt ggttaaagcc tgtaatgcca
    19921 gtgccagagt tgccgacaac ctcccacaga tactggctga ttacttgaaa gaaacaaccg
    19981 gaaacgtacc ggaaaagttg ctacagaaga aaggcgagta acagacgcgc aattattgaa
    20041 caaaaatgag aaaggatctg ataatggcat tttctggcta aaatgcctcg tgtcagatcc
    20101 tttctttttt taatattttc attgttaaac agtctgttac agagaagaag atcggatctg
    20161 acttttgccg gaaaattctc ataaacagat aaaaaccgcg aggtcgccgc cccgtaacgg
    20221 gccataattc caggaaggac ccgacgacac cagactatca gagcgatggg ggcacaatga
    20281 cagaagccga actactggga ttaatccgcc gcgtcgccgg aatcagccag cagcatgacg
    20341 aacaggccac acagccggac agcgtgaccg ctgaaaatta tgtgcgtgtt gtagcggaag
    20401 tgatgcgccg tgatggtata gaacttaatg gcgttgatat gcgcaacata cggaaccgcg
    20461 ttcttgaaat gctggcctac aatcgccgcg ttgaactgta tcgggagaaa gaaaaaataa
    20521 cgtaccactg gaagaagccg gaacggctgc ggcggtaact tgctgatatt cccgataacg
    20581 caaaattgcg ctggctacat cctcacatct gaggactgtt aatcagaagg ccggaatata
    20641 ttcacaaact acgaaatttt cgtagtttaa ttaatggtca gattaatcgc taacccgctg
    20701 atattttcgg aaacctcaat ttgaggaagt cggaagcgtc acccttcata ttctgaatgg
    20761 tcataatgac cacgcagacc atcaaggcaa ccacaacgaa catcgttacg gttggagctt
    20821 ttaccccact gggggaaagg tattacgata atcataacac ctaccgaaca ggtaaaaccc
    20881 accagcctga ttaacagtta accggaaaaa aaatccggta gccccgttat ggggatcccc
    20941 atatcgacat taacgcccct catgaattgg tgcgcttccc cctggaaaga ttacccgcct
    21001 gtatatttct tgtgtctatt tgttccacgt tgtttcatac agtgcaccga acggtgtagt
    21061 taatggtgta gtcaatttgc tgtttttagc actttttgca gtgtgggttg tttaattaaa
    21121 tcaatgggtt gccttgctgt ggtgataaaa ggacatattt attcatcttt cggcgtagaa
    21181 tgctggcgac caaaaatcac ctccatccgc gcaccgccaa gcatgctctc tccggcgacg
    21241 attttaccct catattgctt ggtgatttcg cgggctaccg ccagccccac accttgccca
    21301 gggcgtaaag tatcaacccg ttgaccacgg tcgaaaatga cctctcgctt gcttaatgga
    21361 ataccggggc catcatcctc gaccacaata tagagatgct cgtcggtttg ccttgcagaa
    21421 atttcgacaa actcgaggca atatttacag gcattatcca gtacattgcc catcacctcg
    21481 acaaaatcgt tctgctcacc gacaaagctg atctctggcg aaatatcgag agagatattg
    21541 acccctttgc gttgatacac tttgttcagc gccgaggtga gattgtccag cagtggggcg
    21601 accggatgca gctcgcggct gagcaatgtc ccgccgtgca tactggcacg atgcaggtag
    21661 tagccaattt gctgtgaaat gcggctgatt tgctccagca ttaccggttc agcatcactg
    21721 acgctcatct tttcactacg tagagaacgc agcgtacttt gcagcaccgc cagtggcgtt
    21781 ttcagactat gggtcaggtc ggtgagcgtt gtacgatatt tgtcgtaacg ttcgcgttca
    21841 ctttttaaca atcggttcag gtttcgtacc agactggtca gttctcgcgt tgtggctgga
    21901 ttgagcaatt cgcggttatg ttcttccagt tcgcggactt cttttgccag ggcttcgatg
    21961 gggcgtaaac tccaccaggc ggcgacccac agcagcggga tcactaacag cagattggct
    22021 gagagcacat agataaacca gctccagacc atataggaac tttttagctc caccggaatg
    22081 gtatccacca ccacaatggt taactttggc atccgcgatg ttgccgggta gacgtttacc
    22141 gccaccgagt gggtcatctc cgcgtcgtca tcatcttccc gcacttcctg caactgttgc
    22201 tgtatcgaat gatctccact cagcaagagg ctggtatcgt taacatccgc ttcaatttca
    22261 tgaaaaccat tcgatttcag ccagtcaggc tggatcatct tcatcagcca gggcacgtca
    22321 cgttgcgccc ataaaagctg cccgttctca tcataaatta gcgtcatggt ggggctttgc
    22381 ttgtcgatat tttcgggtaa ctcgacatgc aacttattgt tttcccactt cgcaagggta
    22441 tagaacagat tgctctcgcc acgtaacagc cgaaacgtag ttttatcgaa actgacgcta
    22501 taaccgatca gcgcgaccat tccgtaggca agcgaaagca ccagtactac cgctgccgtt
    22561 gccaacagaa aacgtacccg cagcgagagc gggaaaaaaa gacgcagtaa ttttttcatc
    22621 agcgcaattc gaacaaatag ccctggccgc gaacggtagt aatcacttct tgcggatatt
    22681 gtgcctgaat ttttttgcgc agacgtccca tcagtacatc aatggtatgg ctttcccgca
    22741 gctccgcatc cggatagagt tgtagcatta acgaatcttt gctgaccact ttgccattat
    22801 tgcgtatcaa cgtttccata atggtgtatt cgaacgcggt cagtttgatc acttcgtcat
    22861 taatagataa ttcacggcga gagagatcaa cctgaaacgg gggaagcgaa atgacctgtg
    22921 aagccaggcc gctattacgc cgcattaatg cctgcattcg cgccatcacc tcttcaatat
    22981 gaaacggttt agtcacgtaa tcatcagcac cggcacttaa tacttcgact ttgtcctgcc
    23041 agctttcacg ggcggttaat accagaatcg gcagtgaaac atcattgcta cgccagcggc
    23101 aaatcagtga cagaccgtcc tcgtctggca atccgagatc gacaatcgca atatccggta
    23161 aatgttcatt gagataataa tcggcttctt tggcatcttc tgcatcatcg acctgatgac
    23221 cagcatcctg aatctgaact ttaaggtggt gacgtaacaa cgcattgtct tcaacaacca
    23281 gtacgcgcat ttttatttct ccctgtctta attattaaaa tagtgtaacg cgattatgtg
    23341 gttatggggg taaacattaa ataaaccagc gggggagggg aggtaaagtg aaaaaataaa
    23401 aagcggataa tcttaataag caggccggac agcatcgcca tccggcactg gtacgagttt
    23461 atttcagctc atcaaccatg gtgatggcgc gaccaatgta gttagccggc gtcatcgctt
    23521 tcaggcgggc tttctcttct tctggcaacg ccagaccgtc gataaactgc ttcatgcctt
    23581 cggcgtcaac gcgcttaccg cgagtcagct ctttcagctt ctcgtacggt ttttcgatgc
    23641 catagcgacg cataactgtc tggattggtt ctgccagcac ttcccagttg tgatccagct
    23701 catccagcag atggtcacgg ttcacttcca gtttgctcac gcctttcagg gtggattgat
    23761 acgcaatcag cgcataaccg atacccacgc cgaggttacg cagcacggtg gagtcggtca
    23821 ggtcacgctg ccagcgggaa accggcagtt tgcttgccag atgctgcaat accgcgttgg
    23881 aaaggcccag attcccttcg gagttttcga agtcgatcgg gttaacttta tgcggcatgg
    23941 tggaagaacc aatctcacca gcaatggttt tctgtttgaa gtggttaagg gcgatataac
    24001 cccagacgtc acggtcaaag tcgatcagaa tggtgttgaa gcgcgcaacg caatcaaaca
    24061 gttcggcaat gtagtcgtgc ggttcaatct gggtagtgta cgggttccat tgaataccca
    24121 gcgaggtgac gaactcttcg ctgaactgat gccagtcaac ttccgggtaa gcggcgatgt
    24181 gggcgttata gttaccgacc gcgccgttga ttttgccgag gatctccacc tggttaagct
    24241 ggcggtactg gcgctccata cggtaggcga cgtttgccat ctctttaccg atggttgacg
    24301 gcgtggctgg ctgaccgtgg gtacgggaca gcagcgggat atcgcgatac tgaacggcga
    24361 gatctttaat gccatcaatt agttgacgcc agtacggcag gatcacttca tcacgcgcgg
    24421 ttttcagcat taatgcgtgg gagaggttat tgatatcttc cgaagtacag gcaaagtgga
    24481 tgaattcaga aaccgcgtgc agttccggga tctccgccac tttttctttc aggaaatact
    24541 caaccgcttt aacgtcgtgg ttagtagtac gctcgatggt tttgatgcgt gcggcatctt
    24601 cttcgctgaa attggcgaca attgcatcaa ggtaaccgat tgcgtcggca gcaaaagcag
    24661 gaacttcctt gatcgctgcg tgcgcggcca gtttttgcag ccaacgtact tcaacttgta
    24721 cacggaattt cagcaaacca tattcgctga aaatcccgcg cagcgcgctg actttatcgc
    24781 cgtagcgtcc atcgacaggg gaaacggcgg tcagtgagga taattccata gatcacaact
    24841 ccggggttaa atgagcaaga atttgttttg cctgagtggt caggcgatta cgagaaaaca
    24901 tcagttgcag acgtccgccg ccgacctggt gccagagcac ggcggcgcga atgcctgcca
    24961 gcagggttgc gcgaactttc gcctgcactt gtgggctttg cagtacagca ggggaaccgg
    25021 tgacctgaat gcgcgggcca agcgggctga tcacatcaac atagatagca gccatcgcgc
    25081 tcatcagcgt ttcggactgt aaatcgaagt gttcgagctg gcgttgcagg ccgttgatcc
    25141 ggttgcccag agtgtcgagc gcacctttcg ctgaggagag tttgcgctta agcaccatca
    25201 ggctgagtgt gtagcgggtt aattcggcgt ttaagccctg gcgactgctg gcattgagca
    25261 cgccgagcag ggtttccagc ccgacgcgca ggttggcttc gctaccgcca aaaaccgcca
    25321 gcgtcgagct ggggttcata tcaataatac tgttgagtga gacgtgtagc gcatcggcat
    25381 cacaatgccc ctggtgagcg agttgttgca ccaggcgtgc cgactgacaa ataccggcca
    25441 gggcgagggt gatgtcatag taattctttg ccacgttcac tgcttccttg ttttaagtaa
    25501 agataataat cagatcggca gcggcagacg ctgctcaata ataccgccac cgaggcacac
    25561 ttcaccgtta tagaagacgg cagactggcc cggcgtcacg gcggcaacgg gctcatcgaa
    25621 aatcacttca atgcgatcat cgtccagcgc cttgacggtg caggggatat cggtctggcg
    25681 atagcgggtt tttaccgtgc aacgcatagt accggtgaat ggttcacgat cgacccagtg
    25741 cagctgctgg gcaatcagac cgacagacat cagacgcggg tgttcatggc cctgcgcaac
    25801 aatcagaatg ttgttttcga cgtctttatc gaccacatac cacggttctt cggtgccttc
    25861 tttggtgcca ccgataccca gacctttgcg ctgaccgagg gtgtgataca tcagcccctg
    25921 atgctcacca atttcatcgc catcgactgt aatgattttg cccggttgcg ccgggagata
    25981 acggccaagg aactcgcgga atttacgttc gccaatgaag caaatgccgg tagagtcttt
    26041 tttcttcgcg gtgaccagac caagatcttc agcaatctta cgcacctgcg gtttttccag
    26101 ttcgccgacc gggaacaggc tttgcgcaat ctgctcatgg ctgagcgtat aaaggaagta
    26161 gctctggtct ttattgctgt ccagaccacg cagcaggcgg ctcttgccat cgacatctgc
    26221 ccgacgaacg taatgaccgg tagcgatata atcggcacct aaatcttcgg cggcaaattc
    26281 gaggaaggct ttaaatttga tctctttgtt gcacagaata tccggattcg gcgtgcgacc
    26341 ggctttatat tcggcaagga acagttcgaa gacgttgtcc cagtactcgg cagcaaagtt
    26401 aacggtgtgc agttcaatgc cgagcttgtc gcagacagcc tgggcatcag ccagatccgc
    26461 tgccgctgtg caatattcct caccgtcgtc ttcttcccag ttcttcataa acaggccttc
    26521 gacctgatat ccctgttgtt gcaacagcca ggcagaaacg gaggaatcga caccgccgga
    26581 catgccgacg attacttttt ttgcggtttc agacattgga tcactcacga cattgaactt
    26641 caaggcggcg tattctatca cgcagccttt tagatgacac cctttgtaaa aggccagtta
    26701 aaatcgccaa tcatctccag cggatagcgt tgcccgcttt gataacaacg gatactttcc
    26761 gccaccagcg gcgaacgaag atttgacgcc tgtaaaattt cttcggcgct gacccaacga
    26821 cagcagtcga tatcgctgtc atgaggctgg gtcgggcata tttgctcaag ctcaatggca
    26881 aagaggaaac gcaaaaacgg cgttttatct ggcgcaatcc actgatgcat acgaataaag
    26941 tgttgcggct gcgcgctgat gccggtttct tcccacagct cacgggcggc tgcttccact
    27001 aaggtttcat cggcttccag atgcccggca ggttggttcc ataaggcttt accattaatc
    27061 gtctcttcaa cgactaaaaa tttcccctct gcgtgcacta cgcaagcaac ggtaacgtgc
    27121 ggtttaaaca ttgtgcatcc ttattctgtt gcttctcgcc attcgccatt ggcaaggtta
    27181 tccaaagagt aatcacccat cgcatagcga atcagtcgca gcgtggggaa accaacatgg
    27241 gcggtcatgc ggcgcacctg gcgattacgt ccttcatata aggtgatctt cagccagctg
    27301 gtgggaatac ttttgcgttc acgaattggt ggattccgcg gccataacca cgcgggttcg
    27361 tcaaccagct ccgcgcctgc gggcagggta gggccatcat ttaaggttac gccattgcgc
    27421 aaggcttcaa gtgcgtcttg tgtgggaata ccttccacct gcacataata gatttttccg
    27481 gtgcgtttgc ccggctgggt taaacgcgcc tgcaacgcgc cgttattggt cagcaccagt
    27541 aacccttcgc tatcgcggtc aagacgacct gctgcataaa cgccctgaac cgggatgaat
    27601 tcttttaatg ttttgcgtcc ggcttcatcg gtgaactgcg gaagaacatc gtagggttta
    27661 ttgaacagga tcacacgcgt gggctggttt tcaggtttac gcctggtaga acgttgtgag
    27721 ctgaatcgct taacctggtg atttctaaaa gaagtttttt gcatggtatt ttcagagatt
    27781 atgaattgcc gcattatagc ctaataacgc gcatctttca tgacggcaaa caatagggta
    27841 gtattgacaa gccaattaca aatcattaac aaaaaattgc tctaaagcat ccgtatcgca
    27901 ggacgcaaac gcatatgcaa cgtggtggca gacgagcaaa ccagtagcgc tcgaaggaga
    27961 ggtgaatgga aagtaaagta gttgttccgg cacaaggcaa gaagatcacc ctgcaaaacg
    28021 gcaaactcaa cgttcctgaa aatccgatta tcccttacat tgaaggtgat ggaatcggtg
    28081 tagatgtaac cccagccatg ctgaaagtgg tcgacgctgc agtcgagaaa gcctataaag
    28141 gcgagcgtaa aatctcctgg atggaaattt acaccggtga aaaatccaca caggtttatg
    28201 gtcaggacgt ctggctgcct gctgaaaccc ttgatctgat tcgtgaatat cgcgttgcca
    28261 ttaaaggccc gctgaccact ccggttggtg gcggtattcg ctctctgaac gttgccctgc
    28321 gccaggaact ggatctctac atctgcctgc gtccggtacg ttactatcag ggcactccaa
    28381 gcccggttaa acaccctgaa ctgaccgata tggttatctt ccgtgaaaac tcggaagaca
    28441 tttatgcggg tatcgaatgg aaagctgact ctgccgacgc cgagaaagtg attaaattcc
    28501 tgcgtgaaga gatgggcgtg aagaaaattc gcttcccgga acattgcggt atcggtatta
    28561 agccgtgttc tgaagaaggc accaaacgtc tggttcgtgc agcgatcgaa tacgcaattg
    28621 ctaacgatcg tgactctgtg actctggtgc acaaaggcaa catcatgaag ttcaccgaag
    28681 gcgcgtttaa agactggggc tacgagctgg cgcgtgaaga gtttggcggt gaactgatcg
    28741 acggcggccc gtggctgaaa gttaaaaacc cgaacaccgg caaagagatc gtcattaaag
    28801 acgtgattgc tgatgcattc ctgcaacaga tcctgctgcg tccggctgaa tatgatgtta
    28861 tcgcctgtat gaacctgaac ggtgactaca tttctgacgc cctggcagcg caggttggcg
    28921 gtatcggtat cgcccctggc gcaaacatcg gtgacgaatg cgccctgttt gaagccaccc
    28981 acggtactgc gccgaaatat gccggtcagg acaaagttaa cccaggttct attattctct
    29041 ctgctgagat gatgttacgc catatgggtt ggactgaagc ggctgacctg attgttaaag
    29101 gtatggaagg cgcaatcaat gccaagaccg taacctatga cttcgaacgt ctgatggaag
    29161 gcgctaaact gctgaaatgt tcagagtttg gtgacgcgat catcaagaat atgtaatcac
    29221 tacatgtgtt aaatattgaa acgggcgtat aacacgcccg ttgttttatg atgatttaaa
    29281 atcttcccca aaactttccc caaaaccctt ccccaaaact ggctattttc tatgctgttt
    29341 tgatatctac gataatccag tctttaccac gatcatcatt gtatcggtcg gtcatttttc
    29401 tggatttatg acctaacaac ttttgcgtat ccagaccctg ttcccgatat aaccgttctg
    29461 acagagatcg ctgctcatga aatgtgggcg cagttccttg ctcccatttt atgccacatt
    29521 tttccctggc ctttttaaaa gccgttgtaa gagtatttgc agacacctgg tctcctctgt
    29581 ttgcttgaga ggtagtgtga cggtaatgga ccagatattt actaacaaca gcatccctgc
    29641 actgagatat aacttcacga agggtaatat tcagagcatc gcatttcagg ttaagcggaa
    29701 tagcaagttt tgaaccggtt ttttcctgag taatgtgcaa catgtcgtcc cagatatcag
    29761 agaatttcaa attgcagata tcgcttaaac gttgtccagt gacaagagca agtagcatcc
    29821 cgcattttaa atagggctgc cgtctgctta cgctgtcaaa tattgcctgc cattcgggca
    29881 gtgataatct ttggcggttt actcgatttc gcggttgttt tgttgcctgc gctgggttaa
    29941 atcctggcgg aacatgtcct gcgtgttgtg cttctttgaa gacgtcgatc aacaccattc
    30001 tcacgacttg cgccatcctg ttatgacctt cagcctttac agcatcaatt atttcggcaa
    30061 tatcaagtgc ggtaatatcc ttgaggtgtt gcattccaca atgctcacgg aaaagacgga
    30121 tgggtttgcc tttttgccga taggagttgg gtcttagttc attatgttgc agcctgtcct
    30181 cctggataga attatattta tcaagccatt ctgtcaccgt aatgtctgag cgcctgcctt
    30241 tcattctttc cagacgctca ttgacgctta atatttgtcg ggtacgttgt tcagcaataa
    30301 tggtatttgc ttcagtagca acttgttttg cttcattctc atcagttcct aagctatgaa
    30361 aacgaccgga tagtggatgt ttgtattgcc aatatacctt tccggttcgc ttatctaatt
    30421 tgcaatataa attgggtata gagattttgt gagatcgggg gctagcagcc atcagcgatt
    30481 atccgttgga gttttgggtt tgcgtttatt gggagttgcg gttctgcaag cgttcctaca
    30541 aaacgggaat ttcggtcaat catccagtag cgaccaactt ttatagcggg tggggccatc
    30601 attttccctt gcgcgtattt tttcagaact cgctcacttg gtgctaagtc cccaaattct
    30661 tctttagccc agtcccgtaa agtgattagt cgagacattt gtcctcctct tagctgctga
    30721 gggagtttgt gaccgatata tctgacatga tattaagctc atggcaggta catctcttga
    30781 ctggtcatag agataaattt aatgctgaga aatgcagtat tgaatttatc aatttttcac
    30841 cagcttctgg tctttctggc tgtcatgcgc aaaacatatg gctttaataa aaaactggat
    30901 tgggttagca acgagcaact ttccgaatta accgggatat tgccgcacaa gtgttctgct
    30961 gcaaaaagtg ttctggtaaa gcgtgggatt tttattcaga gcgggcggaa taccggcatt
    31021 aataatgtgg tcagtgaatg gtcaacatta cccgaatcag gtaagaaaaa taaagtttac
    31081 ctgaaagagg taaatttacc tgaatcaggt aaaaaaagtt tacccaaatc aggtaaaggc
    31141 gtttacccga atcaggtaaa cacaaaagac aaactaacaa aagacaatat aaaacctttt
    31201 tcgtccgaga attctggcga atcctctgac caaccagaaa acgatcttcc tgtggtgaaa
    31261 ccggatgctg caattcagag cggcagcaag tgggggacag cagaagacct gaccgccgca
    31321 gagtggatgt ttgacatggt gaagaccatc gcgccatcag ccagaaaacc gaattttgca
    31381 gggtgggcta acgatatccg cctgatgcgt gaacgtgacg gacgtaacca ccgcgacatg
    31441 tgcgtgctgt tccgctgggc atgccaggac aacttctggt ccggtaacgt gctaagtccg
    31501 gccaaactcc gcgacaagtg gacccagctc gaaatcaacc gtaacaagca acaggcaggc
    31561 atgatagcca gcaaaccaaa actcgacctg acaaacactg actggattta cggggtggat
    31621 ttatgaaaaa catcgccgca cagatggtta actttgaccg tgagcagatg cgtcggatcg
    31681 ccaacaacat gccggaacag tacgacgaaa agccgcaggt acagcaggta gcgcagatca
    31741 tcaacggtgt gttcagccag ttactggcaa ctttcccggc gagtctggct aaccgtgacc
    31801 agaacgaact gaacgaaatc cgccgccagt gggttctggc tttccgggaa aacgggatca
    31861 ccacaatgga acaggttaac gcaggaatgc gcgtagcccg tcggcagaat cgaccatttc
    31921 t
//
LOCUS       DAZFAB010000047        30596 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZFAB010000047 DAZFAB010000000
VERSION     DAZFAB010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30596
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.047"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            245..628
                     /gene="secE"
                     /locus_tag="ACHJWA_002477"
     CDS             245..628
                     /gene="secE"
                     /locus_tag="ACHJWA_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFO4309055.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            630..1175
                     /gene="nusG"
                     /locus_tag="ACHJWA_002478"
     CDS             630..1175
                     /gene="nusG"
                     /locus_tag="ACHJWA_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFO4309056.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1334..1762
                     /gene="rplK"
                     /locus_tag="ACHJWA_002479"
     CDS             1334..1762
                     /gene="rplK"
                     /locus_tag="ACHJWA_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFO4309057.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1766..2470
                     /gene="rplA"
                     /locus_tag="ACHJWA_002480"
     CDS             1766..2470
                     /gene="rplA"
                     /locus_tag="ACHJWA_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFO4309058.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2762..3259
                     /gene="rplJ"
                     /locus_tag="ACHJWA_002481"
     CDS             2762..3259
                     /gene="rplJ"
                     /locus_tag="ACHJWA_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFO4309059.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3326..3691
                     /gene="rplL"
                     /locus_tag="ACHJWA_002482"
     CDS             3326..3691
                     /gene="rplL"
                     /locus_tag="ACHJWA_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFO4309060.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4011..8039
                     /gene="rpoB"
                     /locus_tag="ACHJWA_002483"
     CDS             4011..8039
                     /gene="rpoB"
                     /locus_tag="ACHJWA_002483"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFO4309061.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8116..12339
                     /gene="rpoC"
                     /locus_tag="ACHJWA_002484"
     CDS             8116..12339
                     /gene="rpoC"
                     /locus_tag="ACHJWA_002484"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFO4309062.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            12552..12995
                     /locus_tag="ACHJWA_002485"
     CDS             12552..12995
                     /locus_tag="ACHJWA_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase growth adaptation protein"
                     /protein_id="HFO4309063.1"
                     /translation="MKQEVEKWRPFKHPDGDVRDLSFLDAHQVIYVQHHEDKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKLSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVVFRAFSEIFHHCLKLIEK"
     gene            complement(13430..14563)
                     /gene="thiH"
                     /locus_tag="ACHJWA_002486"
     CDS             complement(13430..14563)
                     /gene="thiH"
                     /locus_tag="ACHJWA_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFO4309064.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDSWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDERRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14560..15330)
                     /gene="thiG"
                     /locus_tag="ACHJWA_002487"
     CDS             complement(14560..15330)
                     /gene="thiG"
                     /locus_tag="ACHJWA_002487"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFO4309065.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLAPQSGPGSRSYFAHATSPLTGFLEASV"
     gene            complement(15332..15532)
                     /gene="thiS"
                     /locus_tag="ACHJWA_002488"
     CDS             complement(15332..15532)
                     /gene="thiS"
                     /locus_tag="ACHJWA_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFO4309066.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLVQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15516..16271)
                     /gene="thiF"
                     /locus_tag="ACHJWA_002489"
     CDS             complement(15516..16271)
                     /gene="thiF"
                     /locus_tag="ACHJWA_002489"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFO4309067.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWGQGCYRCLWPENQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16264..16899)
                     /gene="thiE"
                     /locus_tag="ACHJWA_002490"
     CDS             complement(16264..16899)
                     /gene="thiE"
                     /locus_tag="ACHJWA_002490"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFO4309068.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLSDYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16899..18794)
                     /gene="thiC"
                     /locus_tag="ACHJWA_002491"
     CDS             complement(16899..18794)
                     /gene="thiC"
                     /locus_tag="ACHJWA_002491"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFO4309069.1"
                     /translation="MSATKLTRREQRAQAQHFIDTLEGTAFPNSKRIYITGTQPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGIADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(18875..18975)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19027..19503)
                     /gene="rsd"
                     /locus_tag="ACHJWA_002492"
     CDS             complement(19027..19503)
                     /gene="rsd"
                     /locus_tag="ACHJWA_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFO4309070.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19598..20371
                     /gene="nudC"
                     /locus_tag="ACHJWA_002493"
     CDS             19598..20371
                     /gene="nudC"
                     /locus_tag="ACHJWA_002493"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFO4309071.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRYDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKHLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20411..21475
                     /gene="hemE"
                     /locus_tag="ACHJWA_002494"
     CDS             20411..21475
                     /gene="hemE"
                     /locus_tag="ACHJWA_002494"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFO4309072.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVALYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21485..22156
                     /gene="nfi"
                     /locus_tag="ACHJWA_002495"
     CDS             21485..22156
                     /gene="nfi"
                     /locus_tag="ACHJWA_002495"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFO4309073.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22199..22789
                     /gene="yjaG"
                     /locus_tag="ACHJWA_002496"
     CDS             22199..22789
                     /gene="yjaG"
                     /locus_tag="ACHJWA_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFO4309074.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREADESNIGIIFQQ"
     gene            22976..23248
                     /gene="hupA"
                     /locus_tag="ACHJWA_002497"
     CDS             22976..23248
                     /gene="hupA"
                     /locus_tag="ACHJWA_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFO4309075.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23261..23956
                     /gene="yjaH"
                     /locus_tag="ACHJWA_002498"
     CDS             23261..23956
                     /gene="yjaH"
                     /locus_tag="ACHJWA_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFO4309076.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23958..24383)
                     /gene="zraP"
                     /locus_tag="ACHJWA_002499"
     CDS             complement(23958..24383)
                     /gene="zraP"
                     /locus_tag="ACHJWA_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFO4309077.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24621..25997
                     /gene="zraS"
                     /locus_tag="ACHJWA_002500"
     CDS             24621..25997
                     /gene="zraS"
                     /locus_tag="ACHJWA_002500"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFO4309078.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEVWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANCREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASLEGKGARFTLWLPVN
                     ITRKDPQG"
     gene            25994..27319
                     /gene="zraR"
                     /locus_tag="ACHJWA_002501"
     CDS             25994..27319
                     /gene="zraR"
                     /locus_tag="ACHJWA_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFO4309079.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VRERVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHIIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMLVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LAQSLDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27316..28605)
                     /gene="purD"
                     /locus_tag="ACHJWA_002502"
     CDS             complement(27316..28605)
                     /gene="purD"
                     /locus_tag="ACHJWA_002502"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFO4309080.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPTLQ
                     NVAIGVTDIPALLDFAQNEKVDLTIVGPEAPLVKGVVDTFRAAGMKIFGPTAGAAQLE
                     GSKAFTKDFLARHNIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDDVHQRTMERIIWPTVKGMASEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVEDGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28617..30206)
                     /gene="purH"
                     /locus_tag="ACHJWA_002503"
     CDS             complement(28617..30206)
                     /gene="purH"
                     /locus_tag="ACHJWA_002503"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFO4309081.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTKRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7183 a         7928 c         8371 g         7114 t
ORIGIN      
        1 aaagttctga gctaattgcc gataacattt gacgcaatgc gcactaaaag ggcatcattt
       61 gatgcccttt ttgcacgctt tcgtaccaga acctggctca tcagtgattt tctttgtcat
      121 aatcattgct gagacaggct ctgttgaggg cgtataatcc gaaaagctaa tacgcgtttc
      181 gatttggttt gcctcgcgat cgcggggtga aaatgtttgt agaaaacttc tgacaggttg
      241 gtttatgagt gcgaataccg aagctcaagg aagcgggcgc ggcctggaag cgatgaagtg
      301 ggtcgttgtg gtggcattgc tcttggtggc gattgtcggc aactatcttt atcgcgacat
      361 tatgctgccg ctgcgtgcgc tggccgtagt aattctgatt gctgcagcgg gtggtgtcgc
      421 gctgttaacg acaaaaggta aagctaccgt tgcttttgcc cgtgaagcgc gtaccgaagt
      481 ccgtaaggtc atttggccga ctcgccagga aacattgcac accacgctga ttgtggctgc
      541 ggttaccgca gtaatgtcac tgatcctgtg gggactggat ggtattctgg ttcgcctggt
      601 atcctttatc actggcctga ggttctgaga tgtctgaagc tcctaaaaag cgctggtacg
      661 tcgttcaggc gttttccggt tttgaaggcc gcgtagcaac gtcgctgcgt gagcatatca
      721 aattacacaa catggaagat ttgtttggtg aagtcatggt accaaccgaa gaagtggttg
      781 aaatccgtgg cggtcagcgt cgcaaaagcg aacgtaaatt cttccctggc tacgtcctcg
      841 ttcagatggt gatgaacgac gcgagctggc acctggtgcg cagcgtaccg cgtgtgatgg
      901 gcttcatcgg cggtacttcc gatcgtcctg cgccaatcag cgataaagaa gtcgatgcga
      961 ttatgaaccg cctgcagcag gttggtgata agccgcgtcc gaaaacgctg tttgaaccgg
     1021 gtgaaatggt ccgtgttaat gatggtccgt tcgctgactt caacggtgtt gttgaagaag
     1081 tggattacga gaaatctcgt ctgaaagtgt ctgtttctat cttcggtcgt gcgaccccgg
     1141 tagagctgga cttcagccag gttgaaaaag cctaacccag cgatcaaaaa agcggcgatt
     1201 taatcgttgc acaaggcgcg agattggaat acaatttcgc gccttttgtt tttatgggtc
     1261 ttgcccgtaa aacgattttt tatatcacgg ggagcctctc agaggcgtta ttacccaact
     1321 tgaggaattt ataatggcta agaaagtaca agcctatgtc aagctgcagg ttgcagctgg
     1381 tatggctaac ccgagtccgc cagtaggtcc ggctctgggt cagcagggcg taaacatcat
     1441 ggaattctgc aaagcgttca acgcaaaaac tgattccatc gaaaaaggtc tgccgattcc
     1501 ggtagtaatc accgtttacg ctgaccgttc tttcactttc gttaccaaga ctccgccggc
     1561 agcagttctg ctgaaaaaag cggctggtat caagtctggt tccggtaagc cgaacaaaga
     1621 caaagtgggt aaaatttccc gcgctcagct gcaggaaatc gcgcagacca aagctgccga
     1681 catgactggt gccgacattg aagcgatgac tcgctccatc gaaggtactg cacgttccat
     1741 gggcctggta gtggaggact aagaaatggc taaactgacc aagcgcatgc gtgttatccg
     1801 cgagaaagtt gatgcaacca aacagtacga catcaacgaa gctatcgctc tgctgaaaga
     1861 gctggcgact gctaaattcg tagaaagcgt ggacgtagct gttaacctcg gcatcgacgc
     1921 tcgtaaatct gaccagaacg tacgtggtgc aactgtactg ccgcacggta ctggccgttc
     1981 cgttcgcgta gccgtattta cccagggtgc aaacgctgaa gctgctaaag ctgcaggtgc
     2041 tgaactggta ggtatggaag atctggctga ccagatcaag aaaggcgaaa tgaactttga
     2101 cgttgttatt gcttctccgg atgcaatgcg cgttgttggc cagctgggcc aggttctggg
     2161 tccgcgcggc ctgatgccaa acccgaaagt gggtactgta acgccgaacg ttgctgaagc
     2221 ggttaaaaac gctaaagctg gccaggttcg ttaccgtaac gacaaaaacg gcatcatcca
     2281 caccaccatc ggtaaagtgg actttgacgc tgacaaactg aaagaaaacc tggaagctct
     2341 gctggttgcg ctgaaaaaag caaaaccgac tcaggcgaaa ggcgtgtaca tcaagaaagt
     2401 tagcatctcc accaccatgg gtgcaggtgt tgcagttgac caggctggcc tgagcgcttc
     2461 tgtaaactaa tgcctttacg tgggcggtga ttttgtctac aatcttaccc ccacgtatgc
     2521 taacgaaagt tagcagctaa aaagatttgt tcgttggagc ctggcctatc caggcctccg
     2581 tcgaagaccg caggagtttc gcaagaaact taatcccctg cgtagacggt gacagaacgc
     2641 taagattatt cttttatatt ctggcttgtt tctgctcacc gtaattaaga cgctctctcc
     2701 gtttggagga gtgaagtgag ttccagagat tttctctggc aaacatccag gagcaaagct
     2761 aatggcttta aatcttcaag acaaacaagc gattgttgct gaagtcagcg aagtagccaa
     2821 aggcgcgctg tctgcagtag ttgcggattc ccgtggcgta actgtagata aaatgactga
     2881 actgcgtaaa gcaggtcgcg aagctggcgt atacatgcgt gttgttcgta acaccctgct
     2941 gcgccgtgct gttgaaggta ctccgttcga gtgcctgaaa gacgcgtttg ttggtccgac
     3001 cctgattgca tactctatgg aacacccggg cgctgctgct cgtctgttca aagagttcgc
     3061 gaaagcgaat gcaaaatttg aggtcaaagc cgctgccttt gaaggtgagc tgatcccggc
     3121 gtctcagatc gaccgcctgg caactctgcc gacctacgaa gaagcaattg cacgcctgat
     3181 ggcaaccatg aaagaagctt cggctggcaa actggttcgt actctggctg ctgtacgcga
     3241 tgcgaaagaa gctgcttaat cgcagttatc tttttaacgc attcgcttac gtataaactt
     3301 attctgatat tcaggaacaa tttaaatgtc tatcactaaa gatcaaatca ttgaagcagt
     3361 tgcagctatg tctgtaatgg acgttgtaga actgatctct gcaatggaag aaaaattcgg
     3421 tgtttccgct gctgctgctg tagctgtagc tgctggcccg gttgaagctg ctgaagaaaa
     3481 aactgaattc gacgtaattc tgaaagctgc tggcgctaac aaagttgctg ttatcaaagc
     3541 agtacgtggc gcaactggcc tgggtctgaa agaagctaaa gacctggtag aatctgcacc
     3601 ggctgctctg aaagaaggcg tgagcaaaga cgacgcagaa gcactgaaaa aagctctgga
     3661 agaagctggc gctgaagttg aagttaaata agccaaccct tccggttgca gcctgagaaa
     3721 tcaggctgat ggctggtgac tttttagtca ccagcctttt tgcgctgtaa ggcaccagta
     3781 gcgtttcaca ctgtttgact actgctgtgc ctttcaatgc ttgtttctat cgacgactta
     3841 atatactgcg acaggacgtc cgttctgtgt aaatcgcaat gaaatggttt aagcgtgata
     3901 gcaacaggca ttgcggaaag tgttccattt tccggtcaac aaaatagtgt tgcacaaact
     3961 gtccgctcaa tggacagatg ggtcgacttg tcagcgagct gaggaaccct atggtttact
     4021 cctataccga gaaaaaacgt attcgtaagg attttggtaa acgtccacaa gttctggatg
     4081 taccttatct cctttctatc cagcttgact cgtttcagaa atttatcgag caagatcctg
     4141 aagggcagta tggtctggaa gctgctttcc gttccgtatt cccgattcag agctacagcg
     4201 gtaattccga gctgcaatac gtcagctacc gccttggcga accggtgttt gacgtccagg
     4261 aatgtcaaat ccgtggcgtg acctattccg caccgctgcg cgttaaactg cgtctggtga
     4321 tctatgagcg cgaagcgccg gaaggcaccg taaaagacat taaagaacaa gaagtctaca
     4381 tgggcgaaat tccgctcatg acagacaacg gtacctttgt tatcaacggt actgagcgtg
     4441 ttatcgtttc ccagctgcac cgtagtccgg gcgtcttctt tgactccgac aaaggtaaaa
     4501 cccactcttc gggtaaagtg ctgtataacg cgcgcatcat cccttaccgt ggttcctggc
     4561 tggacttcga attcgatccg aaggacaacc tgttcgtacg tatcgaccgt cgccgtaaac
     4621 tgcctgcgac catcattctg cgtgccctga actacaccac agagcagatc ctcgacctgt
     4681 tctttgaaaa agttatcttt gaaatccgtg ataacaagct gcagatggaa ctggtgccgg
     4741 aacgcctgcg tggtgaaacc gcatcctttg acatcgaagc taacggtaaa gtgtacgtag
     4801 aaaaaggccg ccgtatcact gcgcgccaca ttcgccagct ggaaaaagac gacgtcaaac
     4861 tgatcgaagt cccggttgag tacatcgcag gtaaagtggt tgctaaagac tatattgatg
     4921 agtctaccgg cgagctgatc tgcgcagcga acatggagct gagcctggat ctgctggcta
     4981 agctgagcca gtctggtcac aagcgtatcg aaacgctgtt caccaatgat ctggatcacg
     5041 gcccgtatat ctctgaaacc ttacgtgtcg acccaactaa cgaccgtctg agcgcactgg
     5101 tagaaatcta ccgcatgatg cgccctggcg agccgccgac tcgtgaagca gcggaaagcc
     5161 tgttcgagaa cctgttcttc tccgaagacc gttatgacct gtctgcggtt ggtcgtatga
     5221 agttcaaccg ttctctgctg cgcgaagaaa tcgaaggttc tggtatcctg agcaaagacg
     5281 acatcattga tgttatgaaa aagctcatcg atatccgtaa cggtaaaggc gaagtcgatg
     5341 atatcgacca cctcggcaac cgtcgtatcc gttccgttgg cgaaatggcg gaaaaccagt
     5401 tccgcgttgg cctggtacgt gtagagcgtg cggtgaaaga gcgtctgtct ctgggcgatc
     5461 tggataccct gatgcctcag gatatgatca acgccaagcc gatttccgca gcagtgaaag
     5521 agttcttcgg ttccagccag ctgtctcagt ttatggacca gaacaacccg ctgtctgaga
     5581 ttacgcacaa acgtcgtatc tccgcactcg gcccaggcgg tctgacccgt gaacgtgcag
     5641 gcttcgaagt tcgagacgta cacccgactc actacggtcg cgtatgtcca atcgaaaccc
     5701 ctgaaggtcc gaacatcggt ctgatcaact ctctgtccgt gtacgcacag actaacgaat
     5761 acggcttcct tgagactccg tatcgtaaag tgactgacgg tgttgtaact gacgaaattc
     5821 actacctgtc tgctatcgaa gaaggcaact acgttatcgc ccaggcgaac tccaacctgg
     5881 atgaagaagg ccacttcgta gaagacctgg taacctgccg tagcaaaggc gaatccagct
     5941 tgttcagccg tgaccaggtt gactacatgg acgtatccac ccagcaggtg gtatccgtcg
     6001 gtgcgtccct gatcccgttc ctggaacacg atgacgccaa ccgtgcattg atgggtgcga
     6061 acatgcaacg tcaggccgtt ccgactctgc gtgctgataa gccgctggtt ggtactggta
     6121 tggaacgtgc tgttgccgtt gactccggtg taactgcggt tgctaaacgt ggtggtgtcg
     6181 ttcagtacgt ggatgcttcc cgtatcgtta tcaaagttaa cgaagacgag atgtatccgg
     6241 gtgaagcagg tatcgacatc tacaacctga ccaaatacac ccgttctaac cagaacacct
     6301 gtattaacca gatgccgtgt gtgtctctgg gtgaaccggt tgaacgtggc gacgtgctgg
     6361 cagacggtcc gtccaccgac ctcggtgaac tggcgcttgg tcagaacatg cgcgtagcgt
     6421 tcatgccgtg gaatggttac aacttcgaag actccatcct cgtatccgag cgtgttgttc
     6481 aggaagaccg tttcaccacc atccacattc aggaactggc gtgtgtgtcc cgtgacacca
     6541 agctggggcc agaagagatc accgctgaca tcccgaacgt gggtgaagct gcgctctcca
     6601 aactggatga atccggtatc gtttatattg gtgcggaagt gaccggtggc gacattctgg
     6661 ttggtaaggt tacgccgaaa ggtgaaactc agctgacccc agaagaaaaa ctgctgcgtg
     6721 cgatcttcgg tgagaaagcg tctgacgtta aagactcttc tctgcgcgta ccaaacggtg
     6781 tatccggtac ggttatcgac gttcaggtct ttactcgcga tggcgtagaa aaagacaaac
     6841 gtgcgctgga aatcgaagaa atgcagctca aacaggcgaa gaaagacctg tctgaagaac
     6901 tgcagatcct cgaagcgggt ctgttcagcc gtatccgtgc tgtgctggta gccggtggcg
     6961 ttgaagctga gaagctcgac aaattgccgc gcgatcgctg gctggagctg ggcctgaccg
     7021 acgaagagaa acaaaatcag ctggaacagc tggctgagca gtatgacgaa ctgaaacacg
     7081 agttcgagaa gaaactcgaa gcgaaacgcc gcaaaatcac ccagggcgac gatctggcac
     7141 cgggcgtgct gaagattgtt aaggtatatc tggcggttaa acgccgtatc cagcctggtg
     7201 acaagatggc aggtcgtcac ggtaacaagg gtgtaatttc taagatcaac ccgatcgaag
     7261 atatgcctta cgatgaaaac ggtacgccgg tagacatcgt actgaacccg ctgggcgtac
     7321 cgtctcgtat gaacatcggt cagatcctcg aaacccactt gggtatggct gcgaaaggta
     7381 tcggcgacaa gatcaacgcc atgctgaaac agcagcagga agtcgcgaaa ctgcgtgaat
     7441 tcatccagcg tgcgtacgat ctgggcgctg acgttcgtca gaaagttgac ctgagtacct
     7501 tcagcgatga agaagttatg cgtctggctg aaaacctgcg caaaggtatg ccaatcgcaa
     7561 cgccggtgtt cgacggtgcg aaagaagcag aaattaaaga gctgctgaaa cttggcgacc
     7621 tgccgacttc tggtcagatc cgcctgtacg acggccgcac tggtgaacag ttcgaacgtc
     7681 cggtaaccgt tggttacatg tacatgctga aactgaacca cctggtcgac gacaagatgc
     7741 acgcgcgttc caccggttct tacagcctgg ttactcagca gccgctgggt ggtaaggcac
     7801 agttcggtgg tcagcgtttc ggggagatgg aagtgtgggc gctggaagca tacggcgcag
     7861 catacaccct gcaggaaatg ctcaccgtta agtctgatga cgtgaacggt cgtactaaga
     7921 tgtataaaaa catcgtggac ggcaaccatc agatggagcc gggcatgcca gaatccttca
     7981 acgtattgtt gaaagagatt cgttcgctgg gtatcaacat cgaactggaa gacgagtaat
     8041 tctcgctcaa acaggtcact gctgtcgggg taaaacccgg cagcggattg tgctaactcc
     8101 gacgggagca aatccgtgaa agatttatta aagtttctga aagcgcagac taaaaccgaa
     8161 gagtttgatg cgatcaaaat tgctctggct tcgccagaca tgatccgttc atggtctttc
     8221 ggtgaagtta aaaagccgga aaccatcaac taccgtacgt tcaaaccaga acgtgacggc
     8281 cttttctgcg cccgtatctt tgggccggta aaagattacg agtgcctgtg cggtaagtac
     8341 aagcgcctga aacaccgtgg cgtcatctgt gagaagtgtg gcgttgaagt gacccagact
     8401 aaagtacgcc gtgagcgtat gggccacatc gaactggctt ccccgactgc gcacatctgg
     8461 ttcctgaaat cgctgccgtc ccgtatcggc ctgctgctcg atatgccgct gcgcgatatc
     8521 gaacgcgtac tgtactttga atcctatgtg gttatcgaag gcggtatgac caacctggag
     8581 cgtcagcaga tcctgactga agagcagtat ctggacgcgc tggaagagtt cggtgacgaa
     8641 ttcgacgcga aaatgggtgc ggaagcaatc caggccctgc tgaagagcat ggatctggag
     8701 caagagtgcg aacagctgcg tgaagagctg aacgaaacca actccgaaac caagcgtaaa
     8761 aagctgacca agcgtatcaa actgctggaa gcgttcgttc agtctggtaa caaaccagag
     8821 tggatgatcc tgaccgttct gccggtactg ccgccagatc tgcgtccgct ggttccgctg
     8881 gatggtggtc gtttcgcgac ttctgacctg aacgatctgt atcgtcgcgt cattaaccgt
     8941 aacaaccgtc tgaaacgtct gctggatctg gctgcgccgg acatcatcgt acgtaacgaa
     9001 aaacgtatgc tgcaggaagc ggtagacgcc ctgctggata acggtcgtcg cggtcgtgcg
     9061 atcaccggtt ctaacaagcg tcctctgaaa tctttggccg acatgatcaa aggtaaacag
     9121 ggtcgtttcc gtcagaacct gctcggtaag cgtgttgact actccggtcg ttctgtaatc
     9181 accgtgggtc catacctgcg tctgcatcag tgcggtctgc cgaagaaaat ggcgctggag
     9241 ctgttcaaac cgttcatcta cggcaagctg gaactgcgtg gtcttgctac caccattaaa
     9301 gctgcgaaga aaatggttga gcgcgaagaa gctgtcgttt gggatatcct ggacgaagtt
     9361 atccgcgaac acccggtact gctgaaccgt gcaccgactc tgcaccgtct gggtatccag
     9421 gcatttgaac cggtactgat cgaaggtaaa gctatccagc tgcacccgct ggtttgtgcg
     9481 gcatataacg ccgacttcga tggtgaccag atggctgttc acgtaccgct gacgctggaa
     9541 gcccagctgg aagcgcgtgc gctgatgatg tctaccaaca acatcctgtc cccggcgaac
     9601 ggcgaaccaa tcatcgttcc gtctcaggac gttgtactgg gtctgtacta catgacccgt
     9661 gactgtgtta acgccaaagg cgaaggcatg gtgctgactg gcccgaaaga agcagaacgt
     9721 ctgtatcgct ctggtctggc ttctctgcat gcgcgcgtta aagtgcgtat caccgagtat
     9781 gaaaaagatg ctaacggtga attagtagcg aaaaccagcc tgaaagacac gactgttggc
     9841 cgtgccattc tgtggatgat tgtaccgaaa ggtctgcctt actccatcgt caaccaggcg
     9901 ctgggtaaaa aagcaatctc caaaatgctg aacacctgct accgcattct cggtctgaaa
     9961 ccgaccgtta tttttgcgga ccagatcatg tacaccggct ttgcatatgc agcgcgttct
    10021 ggtgcatctg ttggtatcga tgacatggtc atcccggaga agaaacacga aatcatctcc
    10081 gaggcagaag cagaagttgc tgaaattcag gagcagttcc agtctggtct ggtaactgcg
    10141 ggcgaacgct acaacaaagt tatcgatatc tgggctgcgg cgaacgatcg tgtatccaaa
    10201 gcgatgatgg ataacctgca aactgaaacc gttattaacc gtgacggtca ggaagagaag
    10261 caggtttcct tcaacagcat ctacatgatg gccgactccg gtgcgcgtgg ttctgcggca
    10321 cagattcgtc agcttgctgg tatgcgtggt ctgatggcga agccggatgg ctccatcatc
    10381 gaaacgccaa tcaccgcgaa cttccgtgaa ggtctgaacg tactccagta cttcatctcc
    10441 acccacggtg ctcgtaaagg tctggcggat accgcactga aaactgcgaa ctccggttac
    10501 ctgactcgtc gtctggttga cgtggcgcag gacctggtgg ttaccgaaga cgattgtggt
    10561 acccatgaag gtatcatgat gactccggtt atcgagggtg gtgacgttaa agagccgctg
    10621 cgcgatcgcg tattgggtcg tgtaactgct gaagacgttc tgaagccggg tactgctgat
    10681 atcctcgttc cgcgcaacac gctgctgcac gaacagtggt gtgacctgct ggaagagaac
    10741 tctgtcgacg cggttaaagt acgttctgtt gtatcttgtg acaccgactt tggtgtatgt
    10801 gcgcactgct acggtcgtga cctggcgcgt ggccacatca tcaacaaggg tgaagcaatc
    10861 ggtgttatcg cggcacagtc catcggtgaa ccgggtacac agctgaccat gcgtacgttc
    10921 cacatcggtg gtgcggcatc tcgtgcggct gctgaatcca gcatccaggt gaaaaacaaa
    10981 ggtagcatca agctcagcaa cgtgaagtcg gttgtgaact ccagcggtaa actggttatc
    11041 acttcccgta acaccgaact gaaactgatc gacgaattcg gtcgtaccaa agaaagctac
    11101 aaagtacctt acggtgcggt actggcaaaa ggcgatggcg aacaggttgc tggcggcgaa
    11161 accgttgcaa actgggaccc gcacaccatg ccggttatca ccgaagtaag cggttttgta
    11221 cgctttactg acatgatcga cggccagacc attactcgtc agaccgacga actgaccggt
    11281 ctgtcttcgc tggtggttct ggattccgca gaacgtaccg caggtggtaa agatctgcgt
    11341 ccggcactga aaatcgttga tgctcagggt aacgacgttc tgatcccagg taccgatatg
    11401 cctgcgcagt acttcctgcc gggtaaagcg attgttcagc tggaagatgg cgtacagatc
    11461 agctctggtg acaccctggc gcgtattccg caggaatccg gcggtaccaa ggacatcacc
    11521 ggtggtctgc cacgcgttgc ggacctgttc gaagcacgtc gtccgaaaga gccggcaatc
    11581 ctggctgaaa tcagcggtat cgtttccttc ggtaaagaaa ccaaaggtaa acgtcgtctg
    11641 gttatcaccc cggtagacgg tagcgatccg tacgaagaga tgattccgaa atggcgtcag
    11701 ctcaacgtgt tcgaaggtga acgtgtagaa cgtggtgacg taatttccga cggtccggaa
    11761 gcgccgcacg acattctgcg tctgcgtggt gttcatgctg tgactcgtta catcgttaac
    11821 gaagtacagg acgtataccg tctgcagggc gttaagatta acgataaaca catcgaagtt
    11881 atcgttcgtc agatgctgcg taaagctacc atcgttaacg cgggcagctc cgacttcctg
    11941 gaaggcgaac aggttgaata ctctcgcgtc aagatcgcaa accgcgaact ggaagcgaac
    12001 ggcaaagtgg gtgcaactta ctcccgcgat ctgctgggta tcaccaaagc gtctctggca
    12061 accgagtcct tcatctccgc ggcatcgttc caggagacca ctcgcgtgct gaccgaagca
    12121 gccgttgcgg gcaaacgcga cgaactgcgc ggcctgaaag agaacgttat cgtgggtcgt
    12181 ctgatcccgg caggtaccgg ttacgcgtac caccaggatc gtatgcgtcg ccgtgctgcg
    12241 ggtgaagctc cggctgcacc gcaggtgact gcagaagacg catctgccag cctggcagaa
    12301 ctgctgaacg caggtctggg cggttctgat aacgagtaat cgttaatccg caaataacgt
    12361 aaaaacccgc ttcggcgggt ttttttatgg ggggagttta gggaaagagc atttgtcaga
    12421 atatttaagg aatttctgaa tactcataat caatgtagag atgactaata tcctgaaact
    12481 gactgaacta attgagtcaa actcggcaag gattcgatac tattcctgtg tagctttctt
    12541 aaggaacgag aatgaaacag gaagtggaga agtggcgacc ttttaaacat ccggatggcg
    12601 acgttcgtga tttatcgttt cttgatgctc atcaggttat ctacgttcag catcatgagg
    12661 acaaggagcc tttagagtat cgcttttggg ttacctactc tcttcactgc ttcacaaaag
    12721 attatgaaca tcagacgaac gaagaaaaac tatcgttaat gtaccacgcg cctaaagaat
    12781 ctcgtccctt ctgccagcac cgttataact tagcgcgcac acacttaaaa agaactattt
    12841 tggcgctgcc agaaagcaac gttattcatg ccgggtatgg tagctatgcc gtgattgagg
    12901 tggacttaga cggaggagat aaggcatttt actttgttgt gttcagggct ttcagtgaaa
    12961 tttttcacca ttgcctaaaa cttattgaga aataagcagc ttcctcagcc ctcaaaataa
    13021 caaaacccac cttaaggtgg gtttcgccag agaattatct ctggtattca gaacgccatt
    13081 accggacttt gccttgacct tgcgataatc gcaggttgcg ggatgtctga atttcttcag
    13141 tctgctgcat cctggaagat gagaacatgt gttcttattt tcgtctctat catagttgag
    13201 tatttactct cttacaatca gatctctttc attgctcaac aggcgatggc ttcagacttt
    13261 gcattacgga atttttaaga aaggcagggc gaaacgagga agaagctttt cgccctggac
    13321 aacattcctg ctgacggcac taccataaaa aaacacaacg ctttcaacaa ccttgcgttt
    13381 ttcatcttcc gtagcggctc ggaataaatt ttcgttctta cattccgtct catagtcttt
    13441 gcgaggcgcg tcccagatag ctgtcccagt ctttccatac cggctgcaaa ccctgagcgg
    13501 ttaacgcggc agcaaccgct tccggtctgc gctcgtcgtg cggtgagaac tgttccagct
    13561 cggggtgatt gtcggcatag ccacctggct gcgttttcga aaaagcgctg acgttattaa
    13621 ttgccagcgg aataacgcga tcgcgaaacc acggtgattc ccgcgtggag agtgacagtt
    13681 caatctccgg tgcaagcagc cggaaggcgc agatggtttg cactaactgg cgttcatcca
    13741 taatcgacgc aggctcaatg ccgccagtac atggacgcag gcgtgggaag gagacagagt
    13801 agcggctttg ccagtaatgc tgttgcagcc atagcaaatg ttctgcaacc atatagcagt
    13861 caacgcgcca gctgtcggaa aggccaatta gcgcgccgag gcctatctta tcaatccccg
    13921 cacggcccag ccgatccggc gtttccagcc gccagaagaa gtcctgtttt ttgcctttca
    13981 gatgatggcg ggcataagtc gcctcgtgat atgtctcctg ataaaccatc acgccatcca
    14041 gaccaagttg ctttaactcg gcgtattccg tctccgccag cggctgtact tccatctgta
    14101 gtgaagagaa ctgttcacgc agggcaggga gatgacgacg aaagtaatcc atccccactt
    14161 tcgcctgatg ttcaccggtg actaaaagca gatgttcaaa gcccatctcc cgtatagcgg
    14221 cgctttccct ggcaatatcc gcttcatcca gcgttttgcg cttgatgcgg ttgctcatag
    14281 agaatccgca gtacgtgcag tcgttagcgc aaagattgga aagataaagc gggacgtaga
    14341 aactaactgt gttgccaaat cgctgacggg tcagacgctg cgcccgttgg gccagttgtt
    14401 ccagatagcc actggcggct ggcgataaca gcgccatcat atcgtcgcgg gtgagttgcg
    14461 aggcatttag cgcccgctct acgtcagcag ccgttttgcc gttgatacgc aggcggatgt
    14521 cgtcccagtc cagttgtcgc cagcgatcgc tgaaggtttt cataccgatg cctccagaaa
    14581 tccggtcagc gggctggtgg catgagcaaa ataactgcgg ctgcccggtc cggactgagg
    14641 tgccagcagg cctgcttcta ctgccagacg aaatgccttc gccatgttga cgggatcgtc
    14701 cgcgacggca atcgccgtat tcactaacac cgcgtcggcc cccatttcca gcgcctgcgc
    14761 ggcatggctg ggaacgccga tgccagcatc gacaaccacc ggaactgtgg cctgctggat
    14821 aataatctcc agcatggcgc gggtttccag cccctgattc gagccaatcg gcgcgccgag
    14881 cggcatcacc gctgcacagc cgacttcttc cagacgttta cacaataccg gatcggcccc
    14941 gcagtaaggc agcacgacaa atccctgttg taccagcgtt tcggcggctt tcagggtttc
    15001 gatgggatcg ggcaacagcc agcgggcgtc agggtgaatc tccaatttta accagtttgt
    15061 gcctaacgct tcacgagcca gatgggcggc gaaaatggct tcttccgctg ttttcgcccc
    15121 ggatgtattt ggcagcaggg tcacacccgc cgcgataagc ggttcgagga tagcgtcgtt
    15181 gtgctggcgc aagtcgacac gtttcatcgc cagcgtcacc agctggctgc cggaagcgcg
    15241 gattgcctct accatcagtt gtgaagaggc gaatttcccg gtgccggtaa acagatgtga
    15301 atcaaacgtt ttgtccgcaa tacgtaacat ctcaaccccc tgcaataacc tgaaaaagta
    15361 ggatctggtc gccatcctgc acgatatgtt gcgtccactg ctcacgcggg acgatttgct
    15421 gattaatcgc cagagccgcg cccgcttgtc gttgaacgag ttgctccagt agttcgtgaa
    15481 cagtttgccc ggcggcgcac tgcatcggtt gatcgttaaa caggatctgc attgcttcct
    15541 ccgcataccg ggcaaccact ggcgcggcgc aacgccaggc tgcgccactg gctcgactta
    15601 ccgtcgaaca gtcggagttc tcccgcaggc gtctctatac cgcttaataa cttaatggct
    15661 tccagtgcct gcaaagtgcc cataaccccg accaccgggc caaccacgcc cgccgtgcgg
    15721 cagttgcgtt ctggctcctg gttttctggc cacaggcagc ggtaacaacc ctgcccccag
    15781 ggcggcgtca gtaccatcaa ctgaccgcca aatccgaccg cgctggcggt gataagcggc
    15841 gtgttgagtg ccacgcaggc ggcattaatc tcctggcgag tcgccatatt gtcggtacag
    15901 tcgagcacca catcggcttg tgcaaccgca tcttttaacg cctcacccgt taaccgttgt
    15961 tgtaatgctg tcagttgaat atcgggattc aactgtgtca gtcgctgatg gctgacctgc
    16021 gatttcgggc gatcgatatc ttcagtggta aagaggattt gtcgttgcag attgcttaaa
    16081 tgcacatcgt cgtcatctgc cagtaccagc gtcccgacgc cagcgcccgc caggtatagc
    16141 gcagcaggtg tacccagccc gccaagaccg ataatcagca cctggctgtc gagcagtttt
    16201 tgctgcccgt caagagcgat atcgtcgagc aggatttggc ggctataacg cataaaatca
    16261 cggtcattca tcgccaactc ctgcaatttc cagcaactgt gccgttgcca aacgccagtc
    16321 tgcggcttga gtaatggcgc tgacgacggc gatactgccg acacccgttg ctatcaccgc
    16381 aggcgcgcgt gccagactga taccgccaat cgccacggtg ggataatccg acagtcgctc
    16441 aacatgccgt gccagctgtt ccagcccctg cggtgcagaa ggcatctgtt tggtttgcgt
    16501 cgggaacaca tgtcccagcg cgatataaga ggggcgtgct gccagcgcga cgtcgatttc
    16561 catatcgtca tgggtcgaaa cgcccagccg caggcctgcc gcgcggatgg cattgagatc
    16621 ggtggcttgc aaatcttcct gccccaaatg gacgccatac gcctgatgct tgatcgcgag
    16681 ccgccagtaa tcgttgataa acaatcgcgc gttatagcgg cggcccagcg caattgccgc
    16741 cacgacatcg gcttccacct cttcatcgcg ccgatctttg atgcgtagct ggagagtacg
    16801 tacgcctgca tccaacagac gttcgatcca ctgtacgctg tccaccaccg ggtacagtcc
    16861 taaacgaaaa ggtacaggag gaaaatcagg ctgatacatc acgcttcctc cttacgcagg
    16921 tagatttctc cgcctctggc acggaagttc tccgacatat ccgctattcc catttcaata
    16981 gtttgcgtgg cggcgtaatc acgcacttcc tggctgattt tcatcgagca gaatttcggc
    17041 ccacacatgg agcaaaaatg ggcgacttta cctgactctt gcggcagggt ttcatcgtga
    17101 taagcgcggg cggtaaacgg gtcgagggcc agattaaact ggtcttccca gcgaaattcg
    17161 aagcgggctt tcgacatggc gttatcgcga atttgtgcgc ccggatgccc tttcgccagg
    17221 tcagcggcgt gggcggcaat cttataggtg ataagcccct gcttaacatc ttctttatta
    17281 ggcagaccca gatgctcttt tggcgttacg taacagagca tcgcgcagcc aaaccagcca
    17341 atcatcgccg caccaatccc cgacgtgaag tggtcatagc ccggcgcaat atcggtagtt
    17401 agcggcccca gagtgtaaaa cggcgcttcg tggcagtgct ctaactcctc ggtcatattg
    17461 cggcggatca tctgcatcgg cacgtggcct gggccttcaa tcatcacctg cacatcatat
    17521 tcccaggcga tttttgtcag ttcgcccagc gtatgcagct cggcaaactg cgcttcatcg
    17581 ttggcgtcct gaatagaacc ggggcgcaga ccgtcgccca gcgacagcga aacgtcataa
    17641 gcggcacaga tttcacaaat ttcgcggaag tgttgataga ggaaattttc ctgatgatgg
    17701 gagaggcacc atttcgccat aatcgaaccg ccgcgcgaga cgataccggt caggcgtttc
    17761 gcggtcatcg gcacatagcg cagcagcacc cccgcatgga tagtgaagta atccacacct
    17821 tgctcggcct gttccagcag cgtgtcgcgg aacgcttccc aggtaagatc ttcggcgatc
    17881 ccattaacct tctccagcgc ctggtagatc ggcactgtac caatcggcac cgggctgtta
    17941 cgcaaaatcc actcgcgggt ttcgtgaata tagcgaccgg tggagagatc catcaccgta
    18001 tccgctcccc agcgcgtgga ccataccagc ttttccactt cttcttcgat ggaagaggtg
    18061 accgccgagt tgccgatatt ggcgttaact tttaccagga aattgcgacc aataatcatc
    18121 ggctccgatt ccggatgatt aatgttggcc gggataatcg cacgtccggc agcaacttca
    18181 tcacggacaa attccgcagt gatattttcc ggcagacgtg cgccaaagct cattcccgga
    18241 tgctggtggc gtaaaacttc gctacggatg cgctcgcggc ccatattctc gcggatggcg
    18301 atgaattcca tttccggtgt gatgatgccc tggcgggcgt agtgcagttg tgtgacgcgg
    18361 cgtcctgctt tggcgcgttt tggcgttaat acgccgctaa aacgcagctc gtcgaggcca
    18421 tcatctgcca gccgcgcttt agtgtaatcg gaactgcgca cggtaagttc ttcggtatcg
    18481 ccgcgcgcat cgatccacgg ctggcgtagt tttgccagcc cttgctgcac gttaatggcg
    18541 atctgcggat cgccatacgg gccggaggtg tcgtagaccg gaatcgcttc gttttcttcg
    18601 tactgcggct gttctttgct accgccaatt agcgtcgggc taagctggat ctcacgcatc
    18661 ggcacgcgca cgccgggttg tgtgccagtg atataaatgc gttttgagtt gggaaaggcg
    18721 gtgccttcca gggtgtcgat aaaatgttgg gcctgggcgc gttgttcgcg acgggtcagt
    18781 tttgttgcag acatagctca ttccaaaaag ttaaggacgt ggcttgtcag acgacggatg
    18841 aagcaagaga cgatcgccgc aaaggcgatg cgatagcaga ttaactcttg ttcccttcgc
    18901 aggtattagc ctgatcaggt tccgcggatc ccgaataaac ggtctcagcc agttaaggca
    18961 ctccgacaag aaattagccc cacaaacggg gcgttgaatg taaattacgc gttaacagcg
    19021 cagaactcaa gcaggatgtt tgacgcgggc ggcgtcaagc accagcagaa tcagcttatc
    19081 ttccagcaca aagcgcgctt ccagcgcctc gccgatgtca gataaaacct gttgaaactc
    19141 aaggtaatta tcatgatcga tagcggtttc caggctggaa tcgtagtaat ccataatctg
    19201 ttgggtattg gcttcgagtt gcggccaaat cttcgcggcg cgcgcgagtt gcccgttgcc
    19261 ttccagctta tgaagaatgc gctcataaat actgaaatgt ccggcagaca agtaatcgac
    19321 caggctctga caaaaatcat caagggcttt ttcgtttagc ctcatgtacg attctttgcc
    19381 aggcttaatg ccaaccagat tgtagtaagc cacgagcaga tgcttacgta catgtagcca
    19441 gcgatcaacc agtttgttac ttcctctgac gcgctccgtc aggttatcga gctggttaag
    19501 catgattgac tccgcaagtt tgtattcaaa aactgctcag tgagaaatgt aaaaaccatg
    19561 ttaaacatgc cagtgatgca aaggtagtgc aagagctatg gatcgtataa ttgaaaaatt
    19621 agatcacggc tggtgggtcg tcagccatga acaaaaatta tggttgccga agggagaatt
    19681 gccatatggc gaagcggcaa atttcgatct tgtgggtcag cgcgcactac aaatcggcga
    19741 atggcagggg gaacctgttt ggttaataca acagcagcgg cgttacgata tggggtcggt
    19801 acgtcaggtc attgatctcg atgttgggct gtttcaactg gccggacgtg gcgtacaact
    19861 ggcggagttt taccgatcgc ataaatactg tggttactgc gggcatgaaa tgtatccaag
    19921 caaaaccgaa tgggcgatgc tgtgcagcca ttgccgtgag cgttactacc cgcaaatcgc
    19981 cccctgcatt attgttgcca tccgtcgcga tgattcgatc ctcctcgccc agcatacccg
    20041 ccatcgtaac ggtgtccata cggtacttgc cggattcgtc gaagtgggcg aaaccctcga
    20101 gcaggcggtc gcgcgggaag tgatggaaga gagcggaatt aaagttaaac acttgcgtta
    20161 tgtgacttcc cagccgtggc cgtttcctca gtctttaatg accgcgttta tggcggaata
    20221 tgacagcggc gacatcgtga tcgacccgaa agaattgctc gaggcgaact ggtatcgcta
    20281 tgacgatttg ccgttactcc cgccgcccgg caccgtagcg cgccgtctga tagaagatac
    20341 ggtggcgatg tgtcgggcag agtatgagtg atgatacact gaccgcctga cgcactaagg
    20401 aacagccaaa atgaccgaac ttaaaaacga tcgttatctg cgggcgctgc tgcgccagcc
    20461 cgttgatgtc actcctgtat ggatgatgcg ccaggcgggt cgctatctac cggaatataa
    20521 agccacgcgc gcccaggcgg gcgattttat gtcgctgtgc aaaaacgccg agctggcgtg
    20581 tgaagtgacc ttgcaaccgc tgcgtcgcta cccgctggat gcggcgatcc tcttttccga
    20641 tatcctcacc gtcccggacg cgatggggtt agggctctat tttgaagccg gagaaggtcc
    20701 gcgttttacc tcgccagtca cctgcaaagc tgacgtcgat aaactgccaa ttccggaccc
    20761 ggaagatgaa ctggggtacg tgatgaacgc ggtgcgtacc attcgtcgcg aactgaaagg
    20821 cgaagtgccg ctgattggtt tttccggcag cccgtggacg ctggcgacct acatggtgga
    20881 aggcggcagc agcaaagcct tcaccgtgat caaaaaaatg atgtatgccg atccgcaggc
    20941 gctgcacgct ctactcgata aactggcgaa aagcgtcgct ttgtatctga atgcgcagat
    21001 taaagccggt gctcaggctg tgatgatttt cgacacctgg ggcggcgtgc ttaccgggcg
    21061 cgattatcaa cagttctcgc tttattacat gcataaaatt gttgatggtt tactgcgtga
    21121 aaacgacggt cgccgcgtac cggtcacgct gtttaccaaa ggcggcggac agtggctgga
    21181 agccatggca gaaaccggtt gcgatgcgct gggcctcgac tggacaacgg acatcgccga
    21241 tgcgcgccgc cgtgtgggca ataaagtcgc gttgcagggt aatatggatc cgtcgatgct
    21301 gtacgcgccg cctgcccgca ttgaagaaga agtagcgact atacttgcag gtttcggtca
    21361 cggcgaaggt catgtcttta accttggtca cggcattcat caggatgtgc cgccagaaca
    21421 tgctggcgtg ttcgtggagg cagtgcatcg actgtctgaa cagtatcacc gctaaggagc
    21481 gattatggat ctcgcgtcat tacgcgctca acaaatcgaa ctggcttctt ctgtgatccg
    21541 cgaggatcga ctcgataagg atccgccgga tctgatcgcc ggagccgatg tcgggtttga
    21601 gcagggcgga gaagtgacgc gagcggcgat ggtgctgctg aaatacccct cgcttgagct
    21661 ggtggagtat aaagtggccc gcatcgctac caccatgcct tacattccag gttttctttc
    21721 cttccgcgaa tatcctgcgc tgctggcagc gtgggagatg ctgtcacaaa agccggattt
    21781 agtgtttgtc gatggtcatg ggatctcgca tcctcgccgt cttggcgtcg ccagccattt
    21841 tggtttgctg gtggatgtgc cgaccattgg cgtagcgaaa aaacgactat gcggtaaatt
    21901 cgaaccgctc tccagcgaac cgggcgcgct ggccccactg atggataaag gcgagcagct
    21961 ggcctgggtc tggcgcagca aagcgcgctg taacccgcta tttatcgcta ctggtcatcg
    22021 ggtcagcgtg gacagcgcgc tggcgtgggt acaacgctgc atgaaaggct atcgtctgcc
    22081 ggagccaacg cgctgggcgg acgcggtggc ctcggaacgt ccggcgttcg tgcgctatac
    22141 agcaaatcag ccctaattca ggtaaactgc ggataatttc cgtatttgag aactcaacat
    22201 gttacaaaac ccaattcatc tgcgtctgga acgcctggaa agctggcagc atgtcacttt
    22261 catggcttgc ttatgcgaac gcatgtaccc caattacgcc atgttctgcc agcaaaccgg
    22321 ttttggtgat gggcaaattt accgtcgtat tctcgatctc atctgggaaa cgctgaccgt
    22381 taaagatgca aaagtaaatt tcgacagcca actggagaaa tttgaagaag cgattccttc
    22441 agccgacgat ttcgatctgt acggcgttta tccggcaatc gatgcctgcg tggcgttaag
    22501 tgaactggtc cattcgcgtt tgagtggtga aacgctcgaa cacgcggtgg aagtgagtaa
    22561 gacctccatc acgaccgttg cgatgctgga aatgactcag gctggtcgcg aaatgagcga
    22621 tgaagagctc aaagaaaacc cagctgtaga gcaagaatgg gacattcagt gggaaatatt
    22681 ccgactttta gctgagtgcg aagaacgcga tatcgagctg ataaaaggcc ttagggcaga
    22741 cctgcgtgag gcggatgaga gcaatattgg tataattttt cagcaataag accagaaaac
    22801 gtgatttaac gcctgatttg tcgtacctgg agtcttccct ttcgcccccc gtctggtcta
    22861 catttggggg gcgaaaaaaa gtggctatcg gtgcgtgtat gcaggagagt gctattctgg
    22921 catttccgtc gcactcgatg cttagcaagc gataaacaca ttgtaaggat aacttatgaa
    22981 caagactcaa ctgattgatg taattgcaga gaaagcagaa ctgtccaaaa cccaggctaa
    23041 agctgctctg gagtccactc tggctgcaat tactgagtct ctgaaagaag gcgatgctgt
    23101 acaactggtt ggtttcggta ccttcaaagt gaaccaccgc gctgagcgta ctggccgcaa
    23161 cccgcagacc ggtaaagaaa tcaaaattgc cgcagctaac gtaccggcat ttgtttctgg
    23221 caaggcactg aaagacgcag ttaagtaaga ttgcgtggca gtgaacagtt ttaacgaagg
    23281 ggtggtttca ccccttttgt ctttctggcg tcgatcattg atgctggctg gcgctctgtt
    23341 tctcactgct tgtagtcaca actcttcact tcctcccttt accgccagtg gatttgctga
    23401 agaccagggc gcggtacgca tctggcgaaa agacagcggc gataatgtgc atcttcttgc
    23461 cgtgtttagc ccgtggcgca gtggcgatac cacgacgcga gagtatcgct ggcagggcga
    23521 taacctcacg ctcatcaata tcaatgttta cagcaaaccc ccggtgaata ttcgcgcgcg
    23581 ttttgacgat cgcggtgatc tgagctttat gcaacgtgaa tccgatgggg aaaagcagca
    23641 gctttctaac gaccaaatcg atttataccg ttatcgggct gatcagatcc gccagattag
    23701 cgatgcctta cgtcagggga gagtcgtgct gcgccagggg cgctggcatg cgatggaaca
    23761 gaccgtgacc acctgcgaag ggcaaaccat taaacctgat ttagattcgc aggcaatagc
    23821 gcatatcgag cgccgccaga gccgctcttc tgttgatgtc agcgtggcat ggctggaagc
    23881 gcccgaaggt tcgcaattac tgttagtggc aaactctgat ttctgtcgct ggcaacccaa
    23941 cgagaaaacg ttctgattta ccagtggccc atacccatat gaccgccacc accgcagccg
    24001 ccgtagccca tgcccattcc ggctccgcgc ggaatacccg cttcagccat cgcaatatcc
    24061 cgtttcaccc gtaactcatc taacgactga cgcaaattct ccatctcttt ggcgaccgcg
    24121 ttaattttgc tgctatccgg tgggttcgcg gctaacaggg cattgtattc ataacgcttc
    24181 gtcaccagtt gctgttgcag tgcgctgctt tgagcgtaaa agtcattatg gattttctgc
    24241 cacgctgtct gttgttcgct ggtcaaaggc gcggcatttt gctgccacat accgtgtccg
    24301 ccgtgagcaa atgcagatgt cgatcccatc gccattgctg aaagcgccat cattaccagg
    24361 gcaattttcg tgttccgttt catggttaat cctccagtgg ttgtcttact tcgggtattg
    24421 catcttccgt gccaacgatg aaacgctgat atgacgggta atctggcatg ataaacgagt
    24481 aaaaatgact cgcctgctgc gggtagcgag tcatttttac tcattgaaac ttaagccttt
    24541 gtgttacagc gcagggtaag cgctgataaa agatggcatg atttctgctg tcagaaaggg
    24601 atgagcaggc aaagaagaag atgcgtttta tgcaacgttc taaagactcc ttagctaaat
    24661 ggttaagcgc gatcctcccc gtggtcattg ttgggctggt agggttgttt gcggtgacgg
    24721 tgattcgcga ttatggccgc gagactgccg ccgccagaca aacgctgctg gaaaaaggca
    24781 gtgtacttat tcgtgctctc gaatccggct cgcgcgtcgg catggggatg cgcatgcatc
    24841 atgcgcagca gcaggcctta ctggaagaaa tggccgggca gcctggagta cgttggtttg
    24901 cggtcacgga tgaacaagga acaatcgtga tgcatagcaa ctccggcatg gtgggaaaac
    24961 agctttattc cccgcaggaa atgcagcagt tacatccggg agatgaagaa gtgtggcggc
    25021 ggatcgatag cgcagacggc gagcctgttc tggaaattta tcgccagttt caaccgatgt
    25081 ttgctgctgg aatgcaccgg atgcgccata tgcagcagta tgccgcgaca ccacaagcaa
    25141 ttttcatcgc tttcgacgcc agtaatattg tgagtgccga agatcgtgag cagagaaaca
    25201 ccctgattat cctcttcgcc ctggcgacgg tcttgctggc aagcgtattg tcattcttct
    25261 ggtatcgccg ctatctgcgc tcgcgccagc ttctacaaga tgaaatgaag cgcaaagaga
    25321 agctggtggc gctggggcat cttgcggcag gcgttgccca cgaaattcgt aacccgcttt
    25381 cctcgattaa agggctggca aaatactttg ccgagcgcgc gccagcaggg ggagaagcgc
    25441 atcaactggc gcaggtgatg gcgaaagaag ctgaccgttt aaaccgcgtg gtaagcgagc
    25501 tgctggaact ggttaagcca acgcatctgg ctttgcaggc ggtggatctc aacacgctga
    25561 ttaaccactc attacagctg gtaagccagg atgcaaactg ccgggagatc cagttacgct
    25621 ttaccgccaa cgacacgttg ccggaaattc aggccgatcc ggacaggctg actcaggtcc
    25681 tgttgaatct ttatctcaat gctattcagg cgattggtca gcatggcgtg attagcgtga
    25741 cggccagcga aagcggcgcg ggcgtgaaaa tcagcgttac cgacagcggt aagggaattg
    25801 cggcagatca gcttgaagcc atcttcaccc cgtacttcac caccaaagcc gaaggcaccg
    25861 gactggggct ggcggtcgtg cataatattg ttgaacaaca cggtggtaca attcaggtcg
    25921 caagcctgga gggaaaaggc gcaaggttca ccctctggct tccggtcaat attacgcgta
    25981 aggacccaca aggatgacgc acgataatat cgatattctg gtggtggatg atgacattag
    26041 ccactgcact attttgcagg ctttgctgcg cggctggggc tataacgtcg cgctggcgaa
    26101 cagcgggcga caggcgctgg agcaggtgcg ggagcgggtt tttgatcttg tgctttgcga
    26161 tgtgcgaatg gcggagatgg acggcattgc cacgctaaaa gagatcaaag cgttaaatcc
    26221 ggcgattccg gtactgatta tgaccgcata ctctagcgtc gagacggcgg tagaggcgtt
    26281 gaaaaccggg gcgctggatt atctcatcaa gccgctggat ttcgataacc tgcaggcgac
    26341 gctggaaaag gcgctcgcgc atacgcacat tattgatgct gaaacgcctg cggtgactgc
    26401 cagccagttc ggtatggtcg gtaaaagccc ggcgatgcaa cacctgctca gtgaaatcgc
    26461 cctcgtcgcg ccatcggaag ccacggtgct gatccacggc gattccggca ccggtaaaga
    26521 gctggtcgcc agggcgattc acgccagtag cgcacgtagc gaaaaaccgc tggtaacgct
    26581 caactgtgcg gcactcaacg aatccttgct ggaatctgaa ttgttcggtc acgaaaaagg
    26641 ggcgtttact ggagccgata aacgccggga agggcgcttt gttgaggcgg acggcggcac
    26701 gttgtttctc gatgaaattg gcgatatctc gccgatgatg ctggtgcgtc tgctgcgtgc
    26761 gattcaggag cgcgaagttc agcgtgtcgg tagcaaccag actatttcgg ttgatgtccg
    26821 gctgattgcc gcgacccatc gcgatcttgc cgcagaggtg aatgccgggc gttttcgcca
    26881 ggatctctac tatcgcctga acgtggtggc gattgaagta ccatcgctgc ggcaacggcg
    26941 ggaagatatt cctctgctgg ctggccattt tctgcagcgc tttgctgaac gtaatcgcaa
    27001 ggcggtaaaa ggctttacgc cccaggcgat ggatctgttg attcattacg actggccggg
    27061 aaatattcgt gaactggaaa atgcggtgga acgggcggtg gtgctgctga ctggggaata
    27121 tatttccgaa cgcgagctgc cgctggcgat tgccagtacg ccgatcccgc tggcacaaag
    27181 cctggatatt cagccgctgg tggaagtcga aaaagaagtg attctggcgg cactggagaa
    27241 aacgggcggc aacaaaaccg aagccgcccg tcagttaggg atcacgcgca aaacgctgtt
    27301 ggcaaaattg tcgcgttagt tctgctcgcg ttcgatagcg cgccagccga tatctttccg
    27361 gcagaagcag tcgtcccagt ggatatcggt cattaaggca taggcgcgtt tctgtgcttc
    27421 tgctacggta tgacccagcg cggtgacgca cagtacgcgc ccgccgctgg ttaccacctg
    27481 ctcgtcatcc gccagttttg tgcccgcgtg gaacactttg ccgtcttcca cttcttccag
    27541 cggcaggccg tggatcacgt caccggtgcg gtaatcgccc ggatatccac ccgcagccat
    27601 caccacgccg agagaagcac gttcatccca ctctgacgtt ttctcgtcta gtttgccttc
    27661 acaggccgcc aggcagagtt caacaagatc ggacttcatg cgcagcataa tcggctgggt
    27721 ttctggatcg ccaaagcggc agttaaattc gataaccttc ggattgccct gtttgtcgat
    27781 catcaggccc gcgtagagaa aaccggtgta ggtgttgcct tccgacgcca tgcctttcac
    27841 ggttggccag atgatacgtt ccatcgtgcg ctgatgaacg tcatctgtca ctaccggcgc
    27901 aggagagtaa gcgcccattc cgcccgtgtt cggcccggta tctttatcgc ctacgcgttt
    27961 gtgatcctgg ctggtggcca tcggcagcac atgctcgccg tccaccatca cgataaagct
    28021 cgcttcttcg ccgtcgagga actcttcgat aacgatgcga tgacccgcgt cgccaaacgc
    28081 gttgcccgcc agcatatcgt gaacagccgc ttccgcttct tccagcgtca tcgccacgat
    28141 aacgcctttc ccggcagcca gaccgtccgc tttaatgacg attggcgcgc ctttctcacg
    28201 cagatacgcc agcgcaggtt ctacctcggt gaagttctga tattccgccg taggaatgtt
    28261 atggcgggcc aggaaatctt tagtaaacgc tttcgagcct tccagctgtg cggcgcctgc
    28321 ggttgggccg aagattttca tcccggcggc gcggaaggta tcgaccacgc ctttcaccag
    28381 cggcgcttcc gggccgacga tggttagatc taccttttcg ttttgcgcga aatccagcag
    28441 cgccgggata tcggtcacgc cgatagcgac gttttgcagc gtgggttcca gcgctgtgcc
    28501 tgcgttaccc ggagcaacaa aaacagtctc aaccagcggc gactgggccg ctttccaggc
    28561 cagcgcgtgc tcgcgcccgc cgttaccaat cactaatact ttcatctgtc gctccattaa
    28621 tggcggaagt ggcgcatatc ggtgaagagc atcgcaatac cgtgctcgtc ggcggcggca
    28681 atcacttcgt catcacggat ggaaccgcca ggctggatta cgcaggtcac gcccgcagcg
    28741 gcggcggcat caataccgtc gcggaacggg aagaacgcgt cagaagccat cgaggaacct
    28801 ttcacttcca ggccttcatc ggcagcttta ataccggcga ttttcgcgga gtacacgcgg
    28861 ctcatctggc ccgcgccaat accgatggtc atattgtttt tggcatagac gatagcattg
    28921 gatttcacga acttcgccac tttccagcag aacagcgcat cacgcagttc ctgttcggtc
    28981 ggctgacgtt tggtgacgac gcgcagttct tccgcaccga ccatgcccag atcgcgatcc
    29041 tgaaccagca gaccgccgtt cacgcgtttg aaatcaagac ccggaacacg ctcgccccac
    29101 tgaccgcagg tcagaacgcg tacattctgt ttggcggcgg tgattttcag ggcttcttcg
    29161 ctggcggacg gcgcaataat cacttcaaca aactgacgag aaatgatggc ctgtgcggtt
    29221 tccgcatcca gctcgcggtt aaaggcaatg atgccgccga atgcggaggt cgggtcggtt
    29281 ttgtacgcgc gatcgtaagc atcaagaatt gaattgccga tagccacgcc gcaagggttg
    29341 gcgtgcttca caatcacaca tgccggctcg gcgaactctt tcacgcactc cagcgccgca
    29401 tcggtatcgg cgatgttgtt ataagagagg gctttaccct gaacctgggt tgcggtagca
    29461 acggaggctt ctttcacatt ctcttctata tagaaggcag cctgctggtg gctgttctcg
    29521 ccgtaacgca tatcctgctt cttaatgaag ttcaggttca gcgtgcgtgg gaagcgaccg
    29581 gcggcttctt tgctttcacc gtgataagcc ggaaccatgc tgccgaagta gttggcaatc
    29641 atgctgtcgt aggcggcagt gtgttcgaag gctttgatgg cgaggtcgaa acgggttgca
    29701 agcgtcagcg atccttcgtt gtcatccatc tctttaataa tggcgtcgta gtcgctgctc
    29761 ttcaccacga ttgcgacatc tttatggttc ttggcggcgg agcgcaccat cgttgggccg
    29821 ccgatatcga tgttctcaac cgcatcttcc agcgagcaac cttcacgggc cacggtctgg
    29881 gcgaacggat acaggttaac aaccaccata tcgataggct ggatctgatg ttcttccata
    29941 atggcatcgt cctggccgcg acggcccaga atgccaccat gtactttcgg atgcagggtc
    30001 ttcacgcgtc catccatcat ctccgggaaa ccggtgtaat cggaaacttc ggttaccggc
    30061 agaccttttt ctgctaacag acgggcagtg ccccctgtag acagcagctc cacaccgcgt
    30121 gcggaaagtg cctgggcgaa ttcgacgata ccggctttgt cagaaacact gagcagagcg
    30181 cggcggactg gacgacgttg ttgcatggta aatcccctgg atttgactat tacagagagc
    30241 gttagctgaa tttttcgcga aaaactcagc taacgcccct aacggggcat ccttattttt
    30301 cgcccgcatt gtaacgaaaa cgtttgcgca acgctcgcga atttttttaa ttcgatggcg
    30361 atcacgattt tgcccccgat tagcgtggga gaaatagaga aataacgctg atgattgtgg
    30421 ataactctgt gcgtaaaaag gtataaagcg gggttttgct ggggaatgca gcagtcagtc
    30481 atttttctgc aatttttcta ttgcggcctg cggagaactc cctataatgc gcctccatcg
    30541 acacggcgga tgtgaatcac ttcacacaaa cagccggttc ggttgaagag aaaaat
//
LOCUS       DAZFAB010000048        30387 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZFAB010000048 DAZFAB010000000
VERSION     DAZFAB010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.048"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..591)
                     /gene="espY1"
                     /locus_tag="ACHJWA_002504"
     CDS             complement(<1..591)
                     /gene="espY1"
                     /locus_tag="ACHJWA_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspY1"
                     /protein_id="HFO4309082.1"
                     /translation="MKVSVPGMPATLLNMSNNDIYKMVSGDKMDMKMNIFQRLWETLR
                     HLFWSDKQTEAYKLLFNFVNKKAGNINASKYFTGAVNENEKEKFIHSLELFNELKTCA
                     KNPDEMVAKGNMSWVAQTFGDIELSVTFFIENKEICTQTLQLHKGPGNLGVDLREAYL
                     PGVDMRDCYLGLKTMKGHNKVLYLEPGWNANLDGATL"
     gene            complement(886..1701)
                     /gene="djlA"
                     /locus_tag="ACHJWA_002505"
     CDS             complement(886..1701)
                     /gene="djlA"
                     /locus_tag="ACHJWA_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFO4309083.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            1956..4310
                     /gene="lptD"
                     /locus_tag="ACHJWA_002506"
     CDS             1956..4310
                     /gene="lptD"
                     /locus_tag="ACHJWA_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFO4309084.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINANHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVSIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            4363..5649
                     /gene="surA"
                     /locus_tag="ACHJWA_002507"
     CDS             4363..5649
                     /gene="surA"
                     /locus_tag="ACHJWA_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFO4309085.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            5649..6638
                     /gene="pdxA"
                     /locus_tag="ACHJWA_002508"
     CDS             5649..6638
                     /gene="pdxA"
                     /locus_tag="ACHJWA_002508"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFO4309086.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            6635..7456
                     /gene="rsmA"
                     /locus_tag="ACHJWA_002509"
     CDS             6635..7456
                     /gene="rsmA"
                     /locus_tag="ACHJWA_002509"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFO4309087.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPFQES"
     gene            7459..7836
                     /gene="apaG"
                     /locus_tag="ACHJWA_002510"
     CDS             7459..7836
                     /gene="apaG"
                     /locus_tag="ACHJWA_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFO4309088.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            7843..8691
                     /gene="apaH"
                     /locus_tag="ACHJWA_002511"
     CDS             7843..8691
                     /gene="apaH"
                     /locus_tag="ACHJWA_002511"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFO4309089.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     GEAVAS"
     gene            complement(8688..9002)
                     /locus_tag="ACHJWA_002512"
     CDS             complement(8688..9002)
                     /locus_tag="ACHJWA_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="HFO4309090.1"
                     /translation="MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFS
                     RIRPPERLNPILLLVDGKEYVLMTHETATVPVNALGTKFCDASAHRTLIKGALDFMLD
                     GI"
     gene            complement(9005..9238)
                     /locus_tag="ACHJWA_002513"
     CDS             complement(9005..9238)
                     /locus_tag="ACHJWA_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HFO4309091.1"
                     /translation="MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKH
                     AATRWREENAEAIAALNQLADETGCFSDEYRSF"
     gene            complement(9324..9803)
                     /gene="folA"
                     /locus_tag="ACHJWA_002514"
     CDS             complement(9324..9803)
                     /gene="folA"
                     /locus_tag="ACHJWA_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFO4309092.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTRESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(9995..11857)
                     /gene="kefC"
                     /locus_tag="ACHJWA_002515"
     CDS             complement(9995..11857)
                     /gene="kefC"
                     /locus_tag="ACHJWA_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFO4309093.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYICLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(11850..12380)
                     /gene="kefF"
                     /locus_tag="ACHJWA_002516"
     CDS             complement(11850..12380)
                     /gene="kefF"
                     /locus_tag="ACHJWA_002516"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFO4309094.1"
                     /translation="MILIIYAHPYPHHSHANKRMFEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(12488..13819)
                     /gene="yaaU"
                     /locus_tag="ACHJWA_002517"
     CDS             complement(12488..13819)
                     /gene="yaaU"
                     /locus_tag="ACHJWA_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4309095.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYILVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(13877..14164)
                     /gene="fixX"
                     /locus_tag="ACHJWA_002518"
     CDS             complement(13877..14164)
                     /gene="fixX"
                     /locus_tag="ACHJWA_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFO4309096.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(14161..15447)
                     /gene="fixC"
                     /locus_tag="ACHJWA_002519"
     CDS             complement(14161..15447)
                     /gene="fixC"
                     /locus_tag="ACHJWA_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO4309097.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTNVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(15498..16439)
                     /gene="fixB"
                     /locus_tag="ACHJWA_002520"
     CDS             complement(15498..16439)
                     /gene="fixB"
                     /locus_tag="ACHJWA_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFO4309098.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINAFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYASVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(16454..17224)
                     /gene="fixA"
                     /locus_tag="ACHJWA_002521"
     CDS             complement(16454..17224)
                     /gene="fixA"
                     /locus_tag="ACHJWA_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFO4309099.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            17698..19212
                     /gene="caiT"
                     /locus_tag="ACHJWA_002522"
     CDS             17698..19212
                     /gene="caiT"
                     /locus_tag="ACHJWA_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFO4309100.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFLWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            19243..20385
                     /gene="caiA"
                     /locus_tag="ACHJWA_002523"
     CDS             19243..20385
                     /gene="caiA"
                     /locus_tag="ACHJWA_002523"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFO4309101.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            20514..21731
                     /gene="caiB"
                     /locus_tag="ACHJWA_002524"
     CDS             20514..21731
                     /gene="caiB"
                     /locus_tag="ACHJWA_002524"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFO4309102.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKARETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            21805..23358
                     /gene="caiC"
                     /locus_tag="ACHJWA_002525"
     CDS             21805..23358
                     /gene="caiC"
                     /locus_tag="ACHJWA_002525"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFO4309103.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDTFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYDAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFIDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            23467..24252
                     /gene="caiD"
                     /locus_tag="ACHJWA_002526"
     CDS             23467..24252
                     /gene="caiD"
                     /locus_tag="ACHJWA_002526"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFO4309104.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEESYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            24258..24848
                     /gene="caiE"
                     /locus_tag="ACHJWA_002527"
     CDS             24258..24848
                     /gene="caiE"
                     /locus_tag="ACHJWA_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFO4309105.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYTDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(24967..25362)
                     /gene="caiF"
                     /locus_tag="ACHJWA_002528"
     CDS             complement(24967..25362)
                     /gene="caiF"
                     /locus_tag="ACHJWA_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFO4309106.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(25623..28844)
                     /gene="carB"
                     /locus_tag="ACHJWA_002529"
     CDS             complement(25623..28844)
                     /gene="carB"
                     /locus_tag="ACHJWA_002529"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFO4309107.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(28862..30010)
                     /gene="carA"
                     /locus_tag="ACHJWA_002530"
     CDS             complement(28862..30010)
                     /gene="carA"
                     /locus_tag="ACHJWA_002530"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFO4309108.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT         7394 a         8169 c         7706 g         7118 t
ORIGIN      
        1 cagggtagca ccgtccaggt tagcattcca cccaggttcc aagtaaagga ctttattgtg
       61 acctttcatt gttttgagac caaggtaaca atccctcatg tcaacgccgg gaaggtaagc
      121 ctctctaaga tcaacgccca agttacctgg gcctttatgc agctgcaacg tctgagtaca
      181 tatctcctta ttttcaataa aaaaagtgac acttaactcg atatcaccga aggtctgtgc
      241 gacccagctc atattgccct ttgcgaccat ctcatccgga ttttttgcgc atgttttgag
      301 ttcattgaat aattccagac tatggataaa cttttctttc tcattctcgt tgacagcccc
      361 agtaaagtat tttgaggcgt tgatgttgcc agccttttta ttcacgaaat tgaacagaag
      421 tttataagcc tcagtctgtt tatctgacca gaacagatga cgtaacgtct cccacaagcg
      481 ttgaaagata ttcatcttca tgtccatctt gtccccgctc accatcttat aaatatcgtt
      541 attgctcata tttaaaagtg tagccggcat gcctggaact gatactttca tggtaatagc
      601 ctcgcattga gttaaaaact catgatgcgc gctctgaata aatatacttt caaaaatcgc
      661 tcattcagat atgtgttttt tatttttaag acgtaaaaat ataattccgc tcgccgtaaa
      721 gctctcaacc ttaagcaggg tcaacgcgat aaaaccaaca gaaaccctcc cgtaaaccgt
      781 atattgagcc tgtccaccat tctgagtaaa cgccttttct caaagaagtg accgccaggg
      841 cggccacctt ttatcgtggg cagttactca gcatcattta cagggtcatt taaacccttt
      901 ctgctgctta atcagctcat aggcctgctg aatttcctgc gctttctgct tcgccatctc
      961 catcatctca ggcggcaaac ctttcgccac cagcttatcg ggatggtgtt cactcatcag
     1021 cttacggtag gcacgtttga tggtggtcgc atcatccgtc ggcttcacgc ccagcacatt
     1081 acaggcatct tccagcgttg ggccacgctg cgcttgctgc cagttaccac cgccagattg
     1141 ctgctgataa ccgccgccaa actgtgcacc gccctgcatc atgcgcaaaa actggtcaaa
     1201 ctgggcgcgg gagatcccta attcttcagc aatgacatac agcaccgccc gttcattcgg
     1261 gtgcagtgaa ccatcagcaa acgccgcctg aatctggatc tccagaaaca tacgaattaa
     1321 gtcaaaacga ccaaagcaga cactgcgaaa ctggcgcatc ttttcgcgca gcgggtaatt
     1381 gtctgatttt cccacccgga acgcattttg cgccgcagta cgggaagcgc catgaagatt
     1441 cattcggtcc atcaactggc tggcgatatg aatatcagcc tccgtgacgc gacctttgga
     1501 tttggttaaa tgccccatca cttcaaaagt ggtggcaaaa aacagcgcct gacgctcacg
     1561 ctggttggcg aaccacgcca ttttacggct acgggcttta tcaaacatat ggccaattaa
     1621 caggcctaac actacgcccc aaaagccgcc gcccatcagt aaggccacgg ccacgccaat
     1681 gatttttccc caatactgca tatattcccc aaatcgacac acggatatca gggctatctc
     1741 ccacaatata aaggtgcttt taccgttttc cggctgcggt caattgtggg acatcgccta
     1801 taatttgcat tatcatacct gtcattcaat gccgtgccta acaccactga cgctattcgg
     1861 acaggattaa cactagcgta gagatgacga gtacgttagt ctctgaccgt ttgtcacgcg
     1921 caacgttacc gatgatggaa caataaaatc aacgtatgaa aaaacgtatc cccactctcc
     1981 tggccaccat gattgccacc gccctttata gtcaacaggg actggcagcc gacctcgcct
     2041 cacagtgcat gttgggcgtg ccaagctatg accgtcctct ggtacagggc gataccaatg
     2101 acttacccgt gactatcaat gctaaccacg cgaaagggga ctacccggat gacgccgtgt
     2161 ttactggcag cgtggatatc atgcagggta acagccgtct gcaggccgac gaagtgcagc
     2221 tccatcaaaa agaggcacca ggacaaccgg agccggtacg taccgttgat gcgctcggta
     2281 atgtccatta cgacgataac caggtgatcc tcaaagggcc gaaaggctgg gcgaatctga
     2341 acaccaaaga taccaacgtc tgggaaggtg attaccagat ggtgggtcgc cagggtcgcg
     2401 gtaaagcgga cctgatgaaa caacgtggcg aaaaccgcta taccattctg gataacggta
     2461 gctttacctc ctgtctgccg ggttctgaca cctggagcgt ggtaggtagc gaaattattc
     2521 atgaccgcga agaacaagtt gcggagatct ggaacgcccg ctttaaggtg ggtccggtat
     2581 cgatctttta tagcccctat ttgcagttgc cggtgggtga caaacgtcgc tctggtttct
     2641 tgatcccgaa cgccaagtac accaccacca actactttga gttctacctg ccatattact
     2701 ggaacatcgc gccaaatatg gatgccacca tcacgccgca ttatatgcat cgtcgtggca
     2761 acatcatgtg ggagaacgaa ttccgctacc tctcccaggc gggcgctggc ttgatggaac
     2821 tggactatct gccttcagat aaagtctatg aagatgaaca cccgaacgat gacagttcac
     2881 gtcgttggtt attctactgg aaccactccg gggtcatgga tcaggtgtgg cgtttcaacg
     2941 tcgactacac caaggtcagc gatcctagct acttcaatga tttcgataac aagtacggtt
     3001 ccagtactga cggctacgca acgcaaaaat tcagcgttgg ctatgcggtg caaaacttca
     3061 atgccaccgt ttcaaccaag cagttccagg ttttcagcga acagaacacc agtagctact
     3121 cggcagagcc gcagttagac gttaattact accagaatga tgttggtccg tttgatacgc
     3181 gtatttacgg ccaggcagtg cactttgtta acaccagaga cgacatgcct gaagcaaccc
     3241 gtgttcacct ggaaccgacc atcaatttgc cgctctctaa taactggggc agcatcaata
     3301 ccgaagcgaa gttgctggca acccattatc agcaaaccaa tcttgactgg tataactcca
     3361 gaaacacgac caagctggac gaatccgtta accgcgtaat gccgcaattc aaagttgacg
     3421 gcaaaatggt ctttgaacgc gatatggaaa tgctggctcc gggttatacc caaacgctgg
     3481 aaccgcgcgc gcagtatttg tacgtgccgt atcgcgatca gagcgacatc tataactacg
     3541 actcgtctct gctgcaatct gactactctg gcctgttccg ggaccggact tacggcggtc
     3601 ttgaccgtat tgcctccgct aaccaggtga cgaccggtgt cacatctcgc atatatgatg
     3661 atgctgccgt tgaacgtttt aatatttccg ttggtcaaat ctactatttc acggagtctc
     3721 gcactggcga tgacaacata acatgggaga atgacgacaa aacgggttca ctggtgtggg
     3781 caggcgatac ttactggcgt atctccgagc gttggggatt gcgtggcggg attcagtacg
     3841 atacacgtct ggataacgta gcgaccagta actccagcat tgaataccgt cgggatgaag
     3901 accgtctggt acagctgaat taccgttacg ccagcccgga atatattcag gctacgctgc
     3961 ctaagtacta ttccactgct gagcaatata agaatggtat ttcgcaggta ggtgctgtcg
     4021 ccagctggcc aattgccgat cgttggtcca ttgttggggc ctactactac gacaccaatg
     4081 cgaacaagca agccgactct atgttaggtg tgcaatacag ctcctgctgc tatgcaattc
     4141 gcgtcggtta cgagcggaag ctgaacggtt gggataacga taaacaacat gcggtatatg
     4201 acaacgcaat cggctttaac atcgaacttc gcggcctgag ctccaactac ggtctgggta
     4261 cgcaagagat gctgcgttcg aacattctgc cgtatcaaaa cactttgtga tttgttgatt
     4321 taccacgtaa tccgcagtgc ggttaattga aatggaaaaa gtatgaagaa ctggaaaacg
     4381 ctgcttctcg gtatcgccat gatcgcgaat accagtttcg ctgcccccca ggtagtcgat
     4441 aaagtcgcag ccgtcgtcaa taacggcgtc gtgctggaaa gcgacgttga tggattaatg
     4501 cagtcggtaa aactgaacgc tgctcaggca aggcagcaac ttcctgatga cgcgacgctg
     4561 cgccaccaaa tcatggaacg tttgatcatg gatcaaatca tcctgcagat ggggcagaaa
     4621 atgggagtga aaatctccga tgagcagctg gatcaggcga ttgctaacat cgcgaaacag
     4681 aacaacatga cgctggatca gatgcgcagc cgtctggctt acgatggtct gaactacaac
     4741 acctatcgta accagatccg caaagagatg attatctctg aagtgcgtaa caacgaggtg
     4801 cgtcgtcgca tcaccatcct gccgcaggaa gtcgaatccc tggcgcagca ggtgggtaac
     4861 caaaacgacg ccagcactga gctgaacctg agccacatcc tgatcccgct gccggaaaac
     4921 ccgacctctg atcaggtgaa cgaagcggaa agccaggcgc gcgccattgt cgatcaggcg
     4981 cgtaacggcg ctgatttcgg taagctggcg attgctcatt ctgccgacca gcaggcgctg
     5041 aacggcggcc agatgggctg gggccgtatt caggagttgc ccgggatctt cgcccaggca
     5101 ttaagcaccg cgaagaaagg cgacattgtt ggcccgattc gttccggcgt tggcttccat
     5161 atactgaaag ttaacgacct gcgcggcgaa agcaaaaata tctcggtgac cgaagttcat
     5221 gctcgccata ttctgctgaa accgtcgccg atcatgactg acgaacaggc tcgtgtgaaa
     5281 ctggaacaga ttgctgctga tatcaagagt ggtaaaacga cttttgctgc cgcagcgaaa
     5341 gagttctctc aggatccagg ctctgctaac cagggcggtg atctcggctg ggctacacca
     5401 gatattttcg atccggcctt ccgtgacgcc ctgacccgcc tgaacaaagg tcaaatgagc
     5461 gcaccggttc actcttcatt cggctggcat ttaatcgaac tgctggatac ccgtaatgtc
     5521 gataaaaccg acgctgcgca gaaagatcgt gcataccgca tgctgatgaa ccgtaagttc
     5581 tcggaagaag cagcaagctg gatgcaggaa caacgtgcca gcgcctacgt taaaatcctg
     5641 agcaactaat ggttaaaacc caacgtgttg tgatcactcc cggcgagccc gccgggattg
     5701 gcccggactt agttgtccag cttgcacagc gtgagtggcc ggtcgaactg gttgtttgtg
     5761 ccgatgccac tctccttacc gaccgggcag cgatgctcgg tttgccgctc accctccgcc
     5821 cttattcccc caactcccct gcacaaccgc aaactacggg cacattaacg ctacttcctg
     5881 tcgcgctacg tgaatctgtc actgcggggc agttagcgat tgaaaatggg cattacgtgg
     5941 tggagacgct ggcgcgagcg tgtgatggct gtctgaacgg tgaatttgct gcgctgatca
     6001 caggccccgt gcataaaggc gtcattaacg acgcaggcat tccgtttacc ggtcataccg
     6061 agtttttcga agagcgttcg caggcgaaaa aagtggtgat gatgctggcg accgaagaac
     6121 ttcgcgtggc gctggcaacg acgcatttac cgctgcgcga tatcgcagat gctatcaccc
     6181 ctgcactttt gcacgaagtg attgctattt tgcatcacga tttgcgtaca aaatttggta
     6241 ttgccgaacc gcgcattctg gtctgcgggc tgaatccgca cgcgggcgaa ggcggtcata
     6301 tgggtacgga agagatagac accattattc ctgtgctcga cgagctgcgg gcgcagggga
     6361 tgaaactcaa cgggccgctg cctgccgata ccctgtttca gccgaaatat ctcgataacg
     6421 ccgacgccgt gctggcgatg taccacgatc agggtcttcc cgtgctaaaa taccagggct
     6481 tcgggcgcgg tgtgaacatt acgctgggcc tgccctttat tcgcacatca gtggaccacg
     6541 gcaccgcact tgaactggcg ggacgtggcg aagccgatgt cggcagtttt attacggcgc
     6601 ttaatctcgc catcaaaatg attgttaaca cccaatgaat aatcgagtcc accagggcca
     6661 cttagcccgt aaacgcttcg ggcaaaactt tctcaacgat cagttcgtga tcgacagcat
     6721 tgtttctgcc attaacccgc aaaagggcca ggcaatggtc gaaatcggcc ccggtctggc
     6781 ggcattgacc gagccggtcg gcgaacgtct ggaccagctg acggtcatcg aacttgaccg
     6841 cgatctggcg gcacgtctgc aaacgcatcc attcttaggc ccgaaactga cgatttatca
     6901 gcaggatgcg atgaccttta actttggcga actggccgag aaaatgggtc agccgctgcg
     6961 tgttttcggc aacctgcctt ataacatctc cacgccgttg atgttccatc tgtttagcta
     7021 tactgatgcc attgccgaca tgcactttat gttgcaaaaa gaggtggtga atcgtctggt
     7081 tgcaggaccg aacagcaaag cgtatggtcg attaagcgtc atggcgcaat actattgcaa
     7141 cgtgatcccg gtactggaag ttccgccgtc agcctttaca ccaccaccca aagtggattc
     7201 cgccgtcgtg cgcctggttc ctcatgcaac gatgcctcac ccggttaaag atgttcgtgt
     7261 gttgagccgc atcaccaccg aagcctttaa ccagcgtcgt aaaaccattc gtaacagcct
     7321 cggcaacctg tttagcgtcg aggtgttaac gggaatgggg atcgacccgg cgatgcgagc
     7381 ggaaaatatc tctgtcgcgc aatattgcca gatggcgaac tatctggcgg agaacgcgcc
     7441 ttttcaggag agttaacgat gatcaattcg ccccgagtgt gtattcaggt tcaaagcgtc
     7501 tacattgagg cacaatcttc acctgataat gaacgttacg tttttgctta taccgtaacc
     7561 atacgcaatc tggggcgagc gccagtgcag ttgttggggc gttactggct gatcaccaat
     7621 ggcaatggcc gtgaaaccga agtccagggc gaaggagtgg ttggcgtcca gccacttatc
     7681 gcgcctggcg aagagtacca gtacaccagc ggtgcaatca ttgaaacccc gctgggcacc
     7741 atgcagggtc actacgaaat gatcgatgaa aatggcgtcc ctttcagcat cgacattccc
     7801 gtattccgac tcgccgttcc cacactcatt cattaaaaga atatggcgac ataccttatt
     7861 ggcgacgttc atggttgtta cgatgaactg atcgcattgc tgcataaagt agaatttacc
     7921 cctgggaaag ataccctctg gctgacgggc gatctggtcg cgcgcggccc gggttcgctg
     7981 gatgttctgc gctatgtgaa atccttaggc gacagcgtac gtctggtgct gggcaatcac
     8041 gatctgcatc tgctggcagt atttgccggg atcagccgca ataaaccgaa agatcgcctg
     8101 acaccgctgc tggaagcgcc ggatgccgac gagctgctta actggctgcg tcgccagcct
     8161 ctgctgcaaa tcgacgaaga gaaaaagctg gtgatggccc acgcagggat cacgccgcag
     8221 tgggatctgc agaccgccaa agagtgcgcg cgcgatgtag aagcggtgct gtcgagtgac
     8281 tcctatccct tctttctcga cgctatgtac ggcgatatgc caaataactg gtcaccggaa
     8341 ttgcgggggc tgggaagact gcggtttatc accaacgcct ttacccgtat gcgtttttgc
     8401 ttcccgaacg gtcaactgga tatgtacagc aaagaatcgc cggaagaggc ccctgcccca
     8461 ctgaaaccgt ggtttgcgat tcctggacct gtcgctgaag aatacagcat cgcctttggt
     8521 cactgggcat cgctggaagg caaaggtacg ccggaaggta tttacgcgct ggataccggc
     8581 tgctgctggg gtgggtcatt aacctgcctg cgctgggaag ataaacagta ttttgtccag
     8641 ccgtcgaacc ggcataagga tttgggtgag ggagaggcgg tcgcgtctta aatcccgtcg
     8701 agcataaaat ctaatgctcc tttaatcaac gttcggtgcg cgctggcatc gcaaaatttc
     8761 gttcccagcg cgttaactgg aacagttgca gtctcatgcg tcatgagcac atattctttg
     8821 ccgtctacca gtaaaaggat tgggttaagc cgttctggtg ggcgaatacg gctaaatcgt
     8881 tcaataggtg ttaatggaat aacaatacgg cgattaatca caccaataat gtcgctggta
     8941 acatcgataa caaagggaaa cgcggcgttc ctgctgcgac tgcggtatac cgtaaattgc
     9001 atcgctagaa gctccggtac tcatcagaga aacatccggt ttcatcagcc aattgattta
     9061 acgcagcgat agcttcagcg ttctcttcac gccaacgtgt tgctgcatgt tttttaagtt
     9121 cagcattgag cgcaaccgta agggtggcgc tcatatttaa gcctgcatca cgagcgcgat
     9181 tgactaactc acggtcgacg gtgacggtca cactttgtgt ggtacgtttt gcagtcatga
     9241 tatcgtgctc ctgtacaatt tatacgtcta gtatacacgg atatctgcac gtatcagtga
     9301 tgccggaatt ctaatataca aaattaccgc cgctccagaa tctcaaaaca atagctgtga
     9361 gagttctgcg catcagcgtc gtggaattcg ctgaataccg attcccagtc atccggctcg
     9421 taatccggaa aatgggtgtc gccttccact tctgcgtcga tatgcgtcag gtacagtttt
     9481 tgcgctttcg gcaggaactg ctcataaacg cgaccgccgc caatcaccat gatttctggt
     9541 acgtcaccac acgccgcgat ggcttcatcc accgacttca cccacgttac gcgatcgtcc
     9601 gtacccggtt gactgctgag gataatattt ttgcgtcctg gcaacggacg accgatggat
     9661 tccctggtat ggcggcccat aattacgggt ttatttaagg tgttgcgttt aaaccaggcg
     9721 agatcggcag gcaggttcca cggcatggcg ttttccatgc cgataacgcg atctaccgct
     9781 aacgccgcaa tcagactgat cattgagatt tcccgataaa aaaaattgtc gccactatac
     9841 gtaaagcgta aaccgtcgtc gactggtgcg aggatgatgt tgaggaaaat tttatattct
     9901 gctggcgagt ccacgctctc tccctggact cgccgcatta caatgaaaca aaaacaaaca
     9961 gttagctgta aagtgtgatt tacgtcactc tttattagga tgagggtttc gtttccggtt
    10021 catccgccat gttgccggta tgtttacctt cttctgttcc ctgccagcca tgtcgttgaa
    10081 ttaatgacag atgttcgcgg tcctcggtga tgatctcact taacatcgcg ctggtgcgtt
    10141 tatagaccgc cgcgcgggct ttggtgtcat tctcaaccat tgccatctct tccaccatct
    10201 gaatattaaa gcggcggaac acatcggcac gttctcgcgc ttcatacggc cccagaccta
    10261 aactttccag cgccagacgc ccggttttca gcgcaccttc aaaggtttca cgctctggct
    10321 tttcaacgcc tgcctgacgc aaacaaatgt agtgatcgac atcgcgggcg cgggcaataa
    10381 tctgcaaatg cgggaaatgt tctttcacca tctccgtcag ttgcaggttg gtttgcggat
    10441 cgtcaatggc gttaatcagc acttccgctt tcgccgctcc cgcagattcc agtaaatcca
    10501 tccgcgtggc atcgccataa aacactttca taccaaattt acgcagcgtt tcgatatggt
    10561 ccggatcgtg atcgagcacc accattttca ccccgctgga gagcagtaaa cgtccggtga
    10621 tctgcccgaa acgaccgaat ccggcaatga ttacccgcgg ctgttcttcg tcgatctcat
    10681 cggcttcacg cgcctcttca gtagaagatt gctcaaggcg attgaggatc accagcagaa
    10741 tcggcgttgc cgccatcgac agcgccaccg ccagggtcag cgatttcgcc cactccggct
    10801 ccagcacatt cgccatctgc gccgcgccaa ataccacaaa ggcaaactca ctgccctgcc
    10861 ctaacaacac cgcaaaccag cgacgctgtt tatttggcac ctgcaacggt cgggcaatca
    10921 gccacagcat ggcgattttg atgatgagga aaccgagcag caaaatgaca atgcgcaatg
    10981 ggttttcaat cagcgtgcca aagtctatcg acatgccaac accgatgaaa aacagcccca
    11041 acagcaaacc tttaaagggt tcgatatcgc tttccagcgc atgacggtat tccgagcttg
    11101 ccagcagtac gcccgccaga aacgcgccca tcgccatcga caagccgacc tcttccagca
    11161 gcaaaccaaa gccaaacaca aggaataacg ccacggcact aaacacttcc cgcaagccag
    11221 agcgggcaac aaaacgcagc gccggacgcg tgacatagcg ccccagcagt accaccagca
    11281 ccagcgcgcc cgccactttt aacgccgaga gagcaaatgc gcccatcgtc gtcgaggcac
    11341 tgctcgccgc cagtagcggg atcatcgcca ccagcgggat tgccgcgata tcctggaaca
    11401 gcagcaccgc aaaggcactg cggcccattt gcgtcaccat cagattgcgt tcattcatcg
    11461 cctgcatggc aatcgccgta gaggagagcg ccagcgtcat gccgatcaat tccgcgacct
    11521 gccagcgcag cccaagtaac atacagaaca gccccagcag gccgccgcaa atcaccatct
    11581 gcaatgcgcc accgccaaac actgccgcac gcagcttcca cagcctttgt ggatcgagtt
    11641 cgaggccgat aataaacagc atcagcacca cgccaatctc ggcaaagtgc agaatagatt
    11701 cggcatcggt caccagtcgc agcccccacg ggccaataat gcagccggcg atcaggtagc
    11761 caagtaccga tcccagacca agacgtactg caatgggtac aatcagcgcc gccgaaccga
    11821 gataaatcag cgcctgaacc agcgtatggc tatccatgat gggcctcctg ccattccagc
    11881 agacgttgct tatagtgacg cgcctgccct tcgagggttt cgtcgtcaca aataaaggtg
    11941 cagtgcatgg caaacggtgg cagccagttc agcccgcagt agattgccgt cgcctgtagc
    12001 ggctgcgaca gcacatcaaa gcccggatgc gcaccaattt caaaatggct ttccccgccg
    12061 ccggtcgtca ccgcccacag caaatgtttg ccatgcagcg ccgtgccgcc gtgaccgtaa
    12121 gcccagccgt gcgagaagac tttatcgatc caaagtttaa ggagcggagg aatgctgtac
    12181 cactgcatcg ggtgctgcca gacgatcaga tcggcgcgag acagcgcctc ctgctcggcg
    12241 gcaatatcga tattgaagtc aggatagagt tgataaagag agcgaatttc gacgccttcc
    12301 agcgtccttg cctgttcaaa catccgttta ttcgcatggg aatgatgcgg atacggatgc
    12361 gcataaatta taagaatcat aggtagcccg cgtgtttacg cctttgtttt cccacaaagt
    12421 gtagtcatat attcacgcgg cttatagtca ataatattga ttcagaaaag agataaatct
    12481 gaacaaatta ccccattctc tgcccgcgga tcgtcatatt gctggtctgc gccagtgaca
    12541 tccctcgagt ctccggggca aacgctacgg aaatcaacaa gccaaacagc gagatacccg
    12601 cccccattag catcgtgtta ctgataccgt aattattgat aaagatcggt agtgcccagg
    12661 tcgaaacaat ggtgccaata cgacttaagg acataatcac gcccacggca gaggcgcgga
    12721 tatccgtcgg gaagagttca ttaggataga gccactgcaa attacccggc ccgccagaga
    12781 aaaaggcata caccgcaaag gccatcacta ccagccagat ccccatatcc gggatcagcc
    12841 ccaaaaccgc cagcgccagc gtcatcatgg caaagctgcc aatcaacaat ggacgccgtc
    12901 cggcagtgtt tagccacagc atcggcggaa tacagccgag cataaagaac aggctaatca
    12961 ccacgttccc cagtgccgcg tttttgccaa cacccaatcc caacaaacca acgatttgcg
    13021 ggccaaaggt gtaaatggcg aacattggga tcacctggca ggtccagatg gcggcaacaa
    13081 acagaacaaa aggaaaatgg cggcgattaa acagatcgcg aaaacgggtt tgctgcggct
    13141 gctcttcatc gaaagccacc ggttcgccaa acagtttgat catcatctct tcgcactctt
    13201 ttactcgccc tttgcgtaat aaccagcggg gagattcagg cagttcgaat cgaccaatca
    13261 aaatcaacaa acaggggatc gccgcgctac ccagcatcca gcgccagccg ccttccacat
    13321 cataaagcca gtagccgacc agatcggcac aggtcgcgcc gacataccac atggcggcga
    13381 taaagctgat ggaaaacgcc cgctgacggg tactggagaa ctcagtgatc atcgaagtgg
    13441 cgatgggata gtctgcaccg atgacaatgc cgataagtac ccgcatcacc aacagttcga
    13501 cgggggatga aacaaacatc gtcgccaccg atatcacgcc gatggcgatg atatcaatga
    13561 ggaacatttt gcgccgtccg actttatcgg aaatataacc aaacagcgat gtgccaacga
    13621 acagcccggc gagcgttccc gcgcccagca agccaatcca gtcagcgtcc agttttagcg
    13681 ccggcgtcag ttgctccagc gccacgccaa tcattaccag tatataacca tccagaaacg
    13741 gaccaccgct tccccacagc aaaatgcggc ggtgaataga ggaaaattta agatcgtcaa
    13801 agtttctgga cggttgcatg ggttattcct gttttttgca atgagagtca gtgcggcgtt
    13861 acgtatcaaa ccaacatcag ccgtaacgga actccacacc aaaggtgccg cgcgggtatt
    13921 cccactgttc cagcgccgac cccagcccca gaatgcgaca ggtgccgcac tccagacatc
    13981 cggcgtaatc gaagcgcaca ctgccgtcat cctgcttctt gtacagacct gcggggcacg
    14041 ctttcaccag cagctccagc gcctgtttat cggcatcggc tttcacaaca atgtgcggat
    14101 gctcttcatc gacattgaat ttattgacgc ccagtttgac gtccacattg acgggagaag
    14161 tcataaaacg gtcactcctt tcatgccatc cttgatcaga ttgatgaagc ccactttctt
    14221 gccgtggcgg aggattttct tgcgcatcag ttccggcgca ctgccgtcaa tggtgaacag
    14281 gtcacgcgcc acgcctaccg ccagttccgg gtagccgcta aacatgcgtg ggttatcaag
    14341 gaaggccggt agtttctggt acatacgcat atcgcgcagc gggccactct caagatgctg
    14401 acgatattcc gcgagttttt gcttactgaa atcgtcgctt ttcatcgctg aaagcacggt
    14461 ttttgctgca gcttccccgg cggcaatcgc cagatccata ccgcgaatgg taaaaccgag
    14521 gttcatacac attccggcgg catcaccggc aatcaatacg ccgtcaccga ccagctccgg
    14581 cagcatgttg atgcctgctt ccggcactac gtgagcggaa tattccacca gcttgccacc
    14641 cgcgatcagc ggtgcaacgg ccggatgttg tttgaaatct tccagcattt gcggcaccga
    14701 ttttttcgcg tcatgcagat gatgcagacc acaaaccagc cccagcgaca gggtgttttc
    14761 attggtataa agaaaaccgc cgcccatcag gccatcggtg ggtgatcccg caaataggca
    14821 agccgccccc tgattaccct gcaactgaaa acggtcttca ataaccgact tcggtaactc
    14881 gatcagttcc ttcacgccaa ccgccacatt cgtcggttta acgcgttttg ccatccccag
    14941 cttttcggca aggatggagt tcactccatc agcaaggatc accgttttcg cttcaatcac
    15001 atcgccatcg gcttctacac cgacgacttt gccatcgcgc tgtacgaggt tatcgacgcg
    15061 gatcccggta attaactgcg cgcccgcttc ttcggcctgc tccatcagcc aggcatcaaa
    15121 tttactgcgc aaaacggagt aagaacgctg ggatggcgag gtttcgtcac cgttgcagta
    15181 gtccatagtc atcgctgact tttccgtcat aaacgcgagt ttttcatggg tgatcaggcg
    15241 ttctaccggg gcggattcgg cgaaaccagg aataatgtgt tccaggctgt gggcatagag
    15301 acgcccgccg gtgacgttct tggcacctgc ggaattgcca cgctcgataa ctaacacttg
    15361 cgccccttcg cgggcgagca ccagtgcggc aaccgaaccg gcaagccctg caccgacgat
    15421 gatggcgtca aagatatctt cggacataag agctccctgt cagcggcagg accaaaatgc
    15481 agccctgcca gagtggatca acgcgctaaa gctgcggtca gcgccggaag gatcttcacg
    15541 gcgtcgccaa caatgccgta atccgcgtac tggaagatcg gcgcattttt atctttattg
    15601 atggcgaaaa tggtttgcga cgcgttagcg ccaaccatgt gctggatctg cccggagatc
    15661 cccacagcca ggtacagttc aggtttcagc atcaggttgg agataccgac atagcgttcg
    15721 tgctccatcc atttttcgtt ttctgccacc ggacgagaac aggccaactc cgcacctatc
    15781 gccttgcaaa gctgttctgc cagcgcaatg ttctctttgc tgccaatacc gcgaccgacg
    15841 ctgaccacca ggcgggcttt gtcgagatcg acgctgttgc tctggcgcgc ctgggttgcc
    15901 gtgcgggtaa tcgccacagc cggagcctgc cactccacgg tgtgcgtttc gccagtgcgt
    15961 gacgcgtctg gctgagccgc atcgaacgtg ccgctgctga tggtcagtac cgcatacggc
    16021 gtggcaatgc gttcttcgcc aatcgccaga ccaccgtaaa ccatatgttt cactgttgct
    16081 ttaccgtcct gtacgctgac ggtgctggca tcgttagaca ccgccgcttt aaggcgataa
    16141 cccagttttg ccgccagtaa tttgccgcga cgggtgtttg gcaacagcac caggccgtct
    16201 gcaccgtgct ggcgaatagt gtcagccatg acactggcgt aatcttcgat catccgatcg
    16261 tccggtttgc cgtttaattt ccagacatga ttagcgccga gctggattgc ctgtgcgccg
    16321 tcggcatcat tgaggacaaa ggcattgatt tgattagcta aagcctgcgc accgttcatc
    16381 agttccggca gacgagaagg ggtatcgctg aatacccaga cttgagaaaa cgtgttcata
    16441 gcatcccctg taattaaatg actttgcgaa gattttcagc aaatgcggcg atctgttctt
    16501 cgccgtcgcc ttcaatcacg atgcgctgac gctcgcgctg tttcggcgcg gcaacctgtt
    16561 gttctgacca tgctgcctct gcgttaaaac cgatatccgc cgccgaccat acctggactg
    16621 gctttttcgc cgcgccgaga atggctttca tcgaaggaat ttgtggggag ttgatatcag
    16681 tggaaacagc aacaaccgca ggcagtggaa tgcttaaggt ttcggtttca tcttccagtt
    16741 cgcgctcaac ggtgagggta tctgccgtca gggagataat tttgctgacg ccgttaactg
    16801 ccggaatatt gaggatttcg cctaccagca aaccaacctg ctgggcataa aggtcggaag
    16861 aaccatcgcc acagaggatc aaatcaaagc ctgctttctg ggcggctgca gccagtgcgc
    16921 tcgccgtttg ttgcggcagt gcctgctcga actggtcatc aatcaccaca atcagttcat
    16981 ccggaccgcg cgatagcaca tctttacgcc ctttggcgtt ggtcagggct ttaccgccca
    17041 cacttagggc cgtcacctgc gcctctgctg cctgttgctt tagctggcaa gccgcttcaa
    17101 tagcgttgag atcgtattgg cttattttgg catcggcttt actgaagtct aacgaaccat
    17161 cagcattatt gaccgcaata tcctgttcat caggcacgca cttatagcaa gtaataatct
    17221 tcattgcatc tccagaaatc atgaaggaaa cgtcgttcat atcggtggtg ttaattacag
    17281 atatcatgcc ctttaatttg gaaataaaac tttaatcacc aatattgaaa atgtcacacg
    17341 cagataaatc caactttcaa tattgttaag ttcctcacca atattgaaaa cacggcgtag
    17401 caaaaagaaa ttttcaatat tgttttatgg atcaccaata ttgaaagtca gatgctattt
    17461 ttttactcgc aacataacat aacaaactga ttaataatca aaattaacga gaaaagcgcc
    17521 ctctattcac ccgaaacaaa aatgtgataa caatcacaga atgcagctta ttgaataccc
    17581 attatgagtt agccattaac gcgtccatga ggttaataat aattatatta aatgttaaca
    17641 aaaataaaac aaacgggaac gcaaaataaa tattcgtttt cacagtggaa ttaactcatg
    17701 aagaatgaaa agagaaaaac gggaatagaa ccgaaggttt tctttccgcc attaataatc
    17761 gtcggcatac tttgttggct tacagtcaga gatctggatg cggcgaatgt cgttattaat
    17821 gctgtattca gttacgtcac caatgtatgg ggatgggcat ttgaatggta tatggtggtg
    17881 atgcttttcg gttggttctg gctggtgttt ggcccgtatg ccaaaaagcg tttaggtaac
    17941 gaaccacctg aatttagtac cgccagttgg atctttatga tgttcgcctc ctgtacgtct
    18001 gctgccgtac tgttctgggg atcgattgag atctactact acatctccac cccgccgttt
    18061 ggcttagaac cgaactcgac aggagcgaaa gagttggggc tggcttacag cttgttcctc
    18121 tggggacctc tgccgtgggc cacttacagc ttcctttcag tcgccttcgc ttacttcttc
    18181 tttgtccgca aaatggaagt gattcgcccc agctccaccc tggtgccgct ggtaggtgaa
    18241 aaacacgcca aagggttgtt cggcactatc gtcgacaact tctatctcgt cgccttgatc
    18301 ttcgcgatgg gtaccagtct gggccttgcc acgccgctgg tgaccgagtg tatgcaatgg
    18361 ttgtttggca ttccgcatac cctgcaactg gacgctatca tcattacctg ctggattatc
    18421 ctcaacgcca tttgcgtcgc ctgcggtctg caaaaagggg tacgtatcgc cagtgacgtg
    18481 cgtagttacc tgagcttcct gatgctgggt tgggtgttca tcgtcagcgg tgccagcttc
    18541 atcatgaact acttcaccga ttcggtgggg atgttgctga tgtatctgcc gcgcatgttg
    18601 ttctataccg atcccatcgc taaaggcggc ttcccgcagg gctggaccgt gttctactgg
    18661 gcatggtggg tgatttacgc catccagatg agtatcttcc tcgcccgcat ctcccgtggt
    18721 cgtactgtgc gtgaactgtg cttcggcatg gtgatgggac tgacagcatc cacctggatc
    18781 ctgtggactg tactcggtag taacactctg ctgttgatag ataaaaacat catcaacatt
    18841 ccaaatctga tcgaacagta cggtgtggcg cgcgccatca tcgaaacctg ggccgctctg
    18901 ccgctcagca ccgccaccat gtggggcttc ttcatcctct gctttattgc caccgttacg
    18961 ctggttaacg cctgctctta taccctggcg atgtccactt gccgcgaagt acgcgatggt
    19021 gaagaaccac cgctgctggt gcgtatcggt tggtcaattc tggttggcat tatcggtatt
    19081 gttttgctgg cgctcggcgg cctgaaaccg attcaaactg ccattatcgc cggaggatgc
    19141 ccgctgttct tcgtcaacat tatggtgacg ctctccttta ttaaagacgc gaaacagaac
    19201 tggaaagatt aattaacccc caaaatatca agaggttgaa agatggattt taatttaaat
    19261 gatgagcagg aactgtttgt cgccggtatc cgcgaactga tggccagcga aaactgggag
    19321 gcctattttg ccgagtgcga ccgtgacagc gtctacccgg aacgttttgt caaagcactg
    19381 gcggatatgg gtatcgacag tctgctgatc cctgaagagc acggtggtct ggacgcgggg
    19441 tttgttactc tcgccgccgt gtggatggag ctgggacgtc tgggggcacc aacctatgta
    19501 ctgtaccagt tgccgggcgg gttcaatacc ttcctgcgcg aaggcacaca agagcagatc
    19561 gacaagatta tggctttccg cggcaccggt aagcagatgt ggaactcagc gattaccgaa
    19621 ccgggcgcgg gctccgacgt gggtagcctg aaaacgactt atacccgtag aaatggtaag
    19681 atttatctta atggtagtaa gtgttttatt accagtagcg cctacacccc gtacatcgtg
    19741 gtgatggcgc gcgacggggc ttctccggac aaacctgtct acaccgaatg gtttgttgat
    19801 atgagcaaac cgggcatcaa agtgaccaaa cttgagaagc tcggtctgcg tatggatagc
    19861 tgctgtgaaa tcacctttga cgatgtggaa ctggacgaga aagacatgtt cggtcgggaa
    19921 ggtaacggct ttaaccgcgt caaagaagag ttcgaccatg aacgtttcct ggtagccctg
    19981 accaactacg gtacggcgat gtgcgccttt gaagatgcgg cgcgctacgc caaccagcgc
    20041 gtgcagtttg gcgaggctat tggtcgtttc cagttgattc aggaaaaatt cgcccacatg
    20101 gcgatcaaat taaactccat gaaaaacatg ctgtatgaag cagcgtggaa agcagacaac
    20161 ggcaccatca cctctggcga tgcagcgatg tgcaaatact tctgcgccaa tgcggcattt
    20221 gaagtggtgg atagcgcaat gcaggtgctg ggcggtgtcg ggattgcggg caaccaccgc
    20281 atcagccgct tctggcgtga cctgcgtgta gaccgcgttt ccggaggatc tgacgaaatg
    20341 cagatcctga cgctgggtcg tgcggtgctg aagcaatacc gctaagtccg tgcgctgcct
    20401 gatgcgacgc tgacgcgtct tatcaggcct acgggagcac gaatgtaggc cggataaggc
    20461 gtttacgccg catccggcag tcatgcgcta cataaatttt tcaggagcta ataatggatc
    20521 atctacccat gccgaaattc gggccgttgg ccggattgcg cgttgtcttc tccggtatcg
    20581 aaattgccgg gccgttcgcc gggcaaatgt tcgcagaatg gggtgcggaa gttatctgga
    20641 tcgagaacgt cgcctgggcc gacaccattc gcgttcaacc gaactacccg caactctccc
    20701 gccgcaattt gcacgcgctg tcgttaaata ttttcaaaga tgaaggccgc gaagcgtttc
    20761 tgaaattaat ggaaaccacc gatatcttca tcgaagccag taaaggtccg gcctttgccc
    20821 gtcgtggcat taccgatgaa gtactgtggc aacacaaccc gaaactggtt atcgctcacc
    20881 tgtccggttt tggtcagtac ggcaccgagg agtacaccaa tcttccggcc tataacacca
    20941 tcgcccaggc cttcagtggt tacctgattc agaacggtga tgttgaccag ccaatgcctg
    21001 ccttcccgta taccgccgat tacttttctg gcctgaccgc caccacggcg gcgctggcag
    21061 cactgcataa agcgcgtgaa accggtaaag gcgaaagtat cgacatcgcc atgtatgaag
    21121 tgatgctgcg tatgggccag tacttcatga tggattactt caacggcggc gaaatgtgcc
    21181 cgcgcatgag caaaggtaaa gatccctact atgccggttg cggtctgtat aaatgtgccg
    21241 acggctacat cgtgatggaa ctggtgggca ttacccaaat tgaagagtgc tttaaagata
    21301 ttggcctcgc acatctactc ggtacgccgg aaatcccgga aggcactcag cttatccacc
    21361 gtatcgaatg cccttacggc ccactggtag aagaaaaact cgatgcctgg ctggcggcac
    21421 ataccatcgc agaagttaaa gaacgctttg ccgaactgaa tatcgcctgt gccaaagtac
    21481 tgaccgtacc ggaactggaa agcaatccac agtatgttgc ccgtgaatca atcactcagt
    21541 ggcaaacgat ggatggtcgc acctgcaaag ggccgaacat catgccgaaa ttcaaaaata
    21601 accccggaca aatctggcgc ggaatgccct cacatggcat ggacacggct gccattttga
    21661 aaaatatcgg ctacagcgaa aacgacattc aggagttggt cagcaaaggt ctggccaaag
    21721 ttgaggacta aacgcgtggc gtcagtcggc ttgcctgact agcgcccgcc caataacaaa
    21781 cgaacaaaaa tggatagagg tgcaatggat atcattggcg gacaacatct acgtcaaatg
    21841 tgggacgatc tggcggacgt ttacggtcat aaaacggcgc tgatttgtga atccagcggc
    21901 ggagtcgtta accggtatag ttatcttgag ttaaatcagg agattaaccg cacggcaaac
    21961 ctgttttata cgctggggat tcgcaaaggc gacaaggttg cactacatct cgacaactgc
    22021 ccggaattta tcttttgctg gttcgggctg gcaaaaattg gcgcgattat ggtgccgatt
    22081 aacgcccgcc tgttacgcga ggaaagtgcg tggatcctgc aaaatagcca ggcgtgcctg
    22141 ctggtgacca gtgcgcaatt ctatcctatg tatcaacaga ttcagcaaga agatgccact
    22201 caattgcggc acatttgcct gacagatgtg gcacttcccg ctgatgatgg cgtgagttcg
    22261 tttactcaac tgaaaaatca acaacctgcc accttgtgct atgcaccgcc gctatcgact
    22321 gacgatacgg cggaaattct cttcacctcc ggcaccacct cccgaccgaa aggtgtggtg
    22381 attacccatt acaacctgcg cttcgctgga tattactccg cctggcagtg tgcactgcgt
    22441 gacgatgacg tctacctgac ggtaatgcct gcgtttcata tcgattgcca gtgtactgcg
    22501 gcgatggcgg cgttttctgc cggggccacc tttgtgctgg tcgagaaata cagcgcccgc
    22561 gccttctggg gacaggtaca gaagtaccgc gccaccatta ccgaatgtat tccgatgatg
    22621 attcgtacgt tgatggtgca gccgccttca gcgaacgatc ggcaacaccg cctgcgggaa
    22681 gtgatgtttt atctcaactt gtcggagcag gaaaaagaca cattttgtga acgcttcggt
    22741 gttcgcttgc tgacgtctta tgggatgacg gaaaccattg tgggcattat cggcgatcgc
    22801 cctggcgata aacgacgctg gccgtcgatt ggtcgggcgg ggttttgcta cgacgcggag
    22861 atccgcgacg atcacaatcg cccgctcccg gcaggtgaga tcggtgaaat ctgtattaaa
    22921 ggcgtaccag ggaaaaccat cttcaaagag tattttctca acccgaaagc cactgcaaaa
    22981 gtgctggaag ctgatggctg gctgcatacc ggcgataccg gataccgcga cgaagaaggc
    23041 tttttttatt tcatcgatcg ccgctgcaat atgatcaaac gcggtggcga gaatgtctcc
    23101 tgcgtggagc tggaaaatat catcgccacc catccaaaaa ttcaggatat cgtggttgtg
    23161 ggtattaaag attcgattcg cgatgaagcc atcaaagcat ttgtggtgct gaatgaaggt
    23221 gaaacattga gcgaagagga atttttccgc ttctgcgaac aaaatatggc gaaatttaaa
    23281 gtgccctctt atctggagat cagaaaagat ctgccacgta attgctcggg gaaaataatt
    23341 agaaagaatc tgaaataaat gaaacggcag ggaactaccc tgccagctaa taatcacgct
    23401 ctaaaacaat atgcattttt tgcagggatg ttgtcgtccc tgaaaaagca aaaatggaga
    23461 aaaggaatga gtgaatcatt acatctgacc cgcaatggat caattctgga aattactctt
    23521 gatcgcccaa aagcgaatgc tattgatgca aaaaccagct ttgaaatggg cgaagtattt
    23581 ctaaatttcc gtgacgatcc gcaattacgt gtcgccatta ttaccggtgc cggagagaaa
    23641 ttcttttccg cgggctggga tttaaaagcg gcagcagaag gcgaagcacc ggatgctgac
    23701 tttggcccgg gtggttttgc gggattaacc gaaattttca atctcgacaa accggttatc
    23761 gcagctgtga acggctacgc ctttggcggc ggtttcgaac tggcgctggc ggcagatttt
    23821 attgtttgtg ccgataacgc cagcttcgcc ctgccggaag ccaaactggg catcgttcct
    23881 gacagcggcg gtgtgctgcg tctgccgaag atcctgccgc ctgccatcgt caatgaaatg
    23941 gtgatgaccg gcagacgaat gggcgcagaa gaggcgctgc gttgggggat agtcaaccgc
    24001 gtggttagcc aggcggaact gatggataac gcccgcgaac tggctcagca gctggttaac
    24061 agcgccccgc tggcgattgc ggcgctgaaa gagatctacc gcaccaccag cgaaatgccg
    24121 gtagaagaat cgtatcgcta tattcgcagc ggcgtgttga aacactatcc atcggttctg
    24181 cattcggaag atgccattga agggccgctg gcgtttgccg agaagcgcga tccggtgtgg
    24241 aaaggacgtt aacaaccgtg agctattacg cctttgaggg gttaattccg gtggttcacc
    24301 cgacggcgtt tgtccatccc agtgccgtct tgattggcga tgtgattgtg ggagccggtg
    24361 tctacatcgg cccactcgcc tcactgcgtg gtgactacgg gcggttgatc gtgcaagcgg
    24421 gagccaatat tcaggatggc tgcattatgc atggctacac cgacactgac accatcgttg
    24481 gggaaaacgg ccatatcggg cacggagcga tcctgcatgg ttgtgtgatt ggtcgcgatg
    24541 cattggtcgg gatgaacagc gtgattatgg atggcgcggt cattggcgaa gagagcattg
    24601 ttgccgccat gagctttgtc aaagcaggct ttcacggcga gaaacgccag ttgttgatgg
    24661 gtacgccagc ccgcgctgta cgcagtgtta gtgacgacga gttacactgg aaacggttga
    24721 ataccaaaga gtatcaggat cttgtcgggc gctgtcatgc atcgttacat gaaacacagc
    24781 cactgaggca aatggaggaa aatcgccccc gtttgcaggg gacgacggat gtgacgccta
    24841 aacggtgatc ttgccggatg cggcgtaaac gccttatcca actctgctgg agagcaaccg
    24901 taggccggat aagatgcgcc agcatcgcat ccggcgacca ggtcgcacgg tatcatcgaa
    24961 atgacattaa cgacgcatac tctttgacaa catcatctgc cacttctgct cgcggttaag
    25021 ttccgtgagc ggaacggcag gaatacgcgg tgtctttctt acgccgacta atttttcccg
    25081 actgttattg cgcaggcgcg cgtaaatttg ctcatcgata tcgaccactt taaccagtcg
    25141 ctgacactgg catccccgcc cttccagctt attagggatc atcttaactt cgcagcttat
    25201 ttctgtgact tccgacagaa tataagtcag ggtattaacc gccttgctgt gttcaatatc
    25261 gaaatgaata gagatctctc ttgctatcac ccaccgattt tcagccatca tccattcggc
    25321 gattaacaag tagagtggtt tttcaacata tccttcacac atacgaactc cagtttcaca
    25381 aattaaaagt ctggctatga ctcgttttga agcaattaaa caaggccata ttaaaattgt
    25441 ggatatatcg atagtatgca acttcaccgt cgataagtgt gagctaaatc ctgcttatgt
    25501 catcaaaaat atcgattccc caaaagattt acttaatggg caaaaaaaac ggtcctcatc
    25561 agagaaccgt attagttatt cgatcaaatt agcggatgaa aaatatccgc catgacacgc
    25621 tattatttga tctgtgcgtg catttcctgc accgaaatta ctttttcagt cgcatcggca
    25681 ttcagcgcca tcgcggtagc gaaaccaccg ttcagggtgg tgtcataatg cactttatat
    25741 tgcagcgcac tgcgacggat cacgcgggag tcttcaatcg cacgacggcc tgaggtggtg
    25801 ttgatgatgt aggtatattc gccattcttg atacggtcct gaatgtgcgg acggccttca
    25861 tgcaccttgt ttaccagacg cggattgata cccgcttcgc ccagcacaat cgccgtgccg
    25921 tgggtcgcat ccagctcgaa gccctgtttc agcagttttg ccgccaggtc caccacgcgt
    25981 tctttatcgc cttcgcgcac ggaaagcagc gcacgaccgt gtttcttcat ggtggagttg
    26041 ctgcccagct gcgctttggc aaacgcttca gcgaaggtgc ggcccacgcc catgacttcc
    26101 ccggtagaac gcatttctgg ccctaacagc gggtcaacgc ccgggaattt attgaacggc
    26161 agcaccactt ctttcaccga gtagtacggc gggataactt ctttggttac gccctgctca
    26221 gccagcgatt tgccagccat cacacgcgcc gccacttttg ccagcggtac gccggtggct
    26281 ttggagacga acggaacggt acgcgccgca cgcgggttaa cttcaatcag gtagacttcg
    26341 ttgtttttca ccgcaaactg cacgttcatc aggccgcgca cctgcaattc gaaggccagt
    26401 ttctgcacct gctggcgcat tacatcctga atttcctgac ttaaggtgta cgctggcagc
    26461 gaacatgcgg agtcaccgga gtgcacgccc gcctgctcga tgtgctccat gatgccgcca
    26521 atcagcacca tttcgccgtc gcagatggca tccacgtcaa cttctactgc gtcatcaagg
    26581 aaatgatcca gcaacactgg cgcatcgtta gacacgctaa ccgccgtctg gaagtagcga
    26641 cgcaggtcag cttcgtcata gacgatttcc atcgcccgac cgccgagaac gtaagacgga
    26701 cgcaccacca gcgggtagcc aatctctttt gccttctcaa ctgccatttc aatagcagta
    26761 acggtggcgt tcgccggttg tttcagtttc agacggtcaa ccgcatgctg gaagcgttca
    26821 cggtcttccg cacggtcgat agcatccggg ctggtgccga taaccggtac gccagcggct
    26881 tccagtgcac gcgccagttt cagcggggtc tgaccgccgt actgaacgat aacgcctttc
    26941 ggcttctcaa tacgcacgat ttccagcaca tcttccagag ttaccggctc gaagtagagg
    27001 cgatcggaag tgtcgtagtc ggtagagacg gtttccgggt tacagttaac cataatggtt
    27061 tcgtaaccgt cttcacgcag cgccagcgag gcgtgtacgc agcagtagtc gaattcgata
    27121 ccctgaccga tacggtttgg accgccgcca agcaccatga ttttttcacg gtcggtagac
    27181 ggattcgctt cgcactcttc ttcataagtg gagtacatgt aagcggtgtc ggtggcaaac
    27241 tctgccgcac aggtatccac gcgcttgtag accgggtgca ggtcatattg gtcacgcagc
    27301 ttacggattt ccgcttcgcg tacgcccgcc agttttgcca agcgcgcatc ggcaaagcct
    27361 ttgcgtttca gctggcgcag gaattcagcg ttcaggccag tgatgcccac ttccgccact
    27421 ttctcttcca gacgcaccag ttcttcaatc tgtaccagga accagcggtc aatgttagtc
    27481 aggttgaaga cgccgtccac agacaggccc gcgcggaaag catcggcgat gtaccagata
    27541 cgctctgcgc ctgcgtcttt cagttcgcga cggattttgg ttaacgcttc cgggtcatcc
    27601 aggctcactt tcgggtcgaa tccagtcgca ccgacttcca ggccgcgcag cgctttttgc
    27661 agggattcct gctgcgtgcg accaatcgcc atcacttcgc caaccgattt catctgagtg
    27721 gtcagacggt cgttagcacc ggcgaatttt tcgaagttga agcgaggaat tttggtaacc
    27781 acgtaatcga tggacggctc gaaggaggcc ggagtacgtc cgccagtgat gtcgttcatc
    27841 agttcatcga gggtgtaacc caccgccagt ttcgccgcca ctttagcaat cgggaaaccg
    27901 gtcgctttcg acgccagcgc cgaagaacgg gacacgcgtg ggttcatttc gataacaatc
    27961 aggcgaccgt ttttcgggtt caccgcaaac tggacattgg aaccaccggt ttcaacgccg
    28021 atttcacgca gcaccgccat cgaggcgtta cgcatgattt gatattcttt gtcggtcagc
    28081 gtttgggctg gcgcgacagt gatggagtca ccggtgtgga tgcccatcgc atcaaagttt
    28141 tcgatagagc agacgatgat gcagttgtcg tttttatcgc gcaccacttc catctcgtac
    28201 tctttccagc cgatcagcga ctcatcaatc agcaactctt tggtcggaga gagatccaga
    28261 ccgcgggcgc aaatttcttc aaactcttcg cggttataag cgataccgcc gccgctaccg
    28321 cccatggtaa aggatgggcg aataatgcac gggaagccca cgtcagcggc aaccgccagc
    28381 gcttcttcca tcgtatgtgc gataccggaa cgcgcggttt ccagaccaat tttcttcatc
    28441 gctacgtcga aacgacggcg gtcttctgct ttatcaatcg catcggcagt ggcaccaatc
    28501 atggtgacgc cgaactcttc caacacgccc tgacgttcca gctccagcgc gcagttcagc
    28561 gccgtctgac cgcccatggt tggcagcacc gcgtccgggc gctctttttc aataatctta
    28621 cgtaccactt cccagtgaat cggctcgatg taggtcgcat cggccatttc cgggtcggtc
    28681 atgatggtcg ccgggttgga gttcaccaga atgacgcggt aaccctcttc gcgcagggct
    28741 ttacatgctt gcgcgccaga gtagtcaaac tcacacgcct gaccgataac aatcgggccc
    28801 gcacccagaa tcaggatact ttttatatct gtacgttttg gcatggctct tttactcctg
    28861 attacttagc ggttttacgg tactgctcaa ttaactcgat aaagtggtcg aacaacggcg
    28921 cggcgtcgtg tggaccaggg ctggcttcag ggtgaccctg gaagctgaac gccggtttat
    28981 cggtgcgatg aatgccctgt aacgtaccgt cgaacaggga tttatgcgtg acacgcaggt
    29041 ttgcaggtaa tgttgcttca tccaccgcaa aaccgtggtt ctgggcggtg atcatcacca
    29101 cgtttttctc cacatcttta accggatggt tgccgccgtg gtgaccaaat ttcattttga
    29161 cagtcttcgc accgctcgcc agcgccagca gctgatgacc gaggcagatg ccgaagaccg
    29221 gaatatcggt ttcgaggaat ttctggatgg cggtaatggc gtaatcgcac ggggccgggt
    29281 cgccaggacc gttggagagg aagatgccgt ctggattcat tttcagcaca tcttccgcag
    29341 aagtttgcgc cggaacgatg gtcaggcgac agcctctatc caccagcatc cgcaggatgt
    29401 tgcgtttggc accaaaatca taagccacga cgtggaacgg cagctcgtct tcttttttcg
    29461 cttctggcag gccgccggtc aacgtccagc tcccttgtgt ccagctatag gcttctgcgg
    29521 tggtcacttc ttttgccaga tccatgccat tcagacctgg gaacgcgcgg gctttttcta
    29581 acgccagcgc cgcatccggg ttatcgcctg cgataatgca gccattctgt gcgcctttct
    29641 cgcgcagtaa acgcgtcagc ttacgggtat cgatatcggc aatcgccacg atgttatggc
    29701 gcttcaggta agaagagagg tcttcggtat tacggaagtt gctggcaatc agcggcaggt
    29761 cgcgaatcac cagaccttgt gcatgtacct gagaagattc ttcatcggcg tcattagtgc
    29821 cgacattgcc aatatgggga taagtaagag taacgatttg acgagaatag gaaggatcag
    29881 tgaggatttc ttgataaccg gtcattgaag tattgaaaac gacttcccca accgccgaac
    29941 ctgttgcccc tatggcccga ccgtgaaact gggttccgtc ttccagaacc aatagcgctg
    30001 acttaatcaa aacaccctcc agagaatatt cactcacttt atttgcatat taattcacaa
    30061 tgatgacgta aatcaatgca aatctgctta ccgacgaatt tctggcaaac ggcggcattc
    30121 tggagatatg ggcgctaaaa gtcaacttaa tggcaggatt ttttatatta ttttgtggct
    30181 catgcatgtt ttacaggcat taagcgacaa aaagatcatc taacctgttc tggaaaacgc
    30241 ttgcgcagca agaagattgc atgaaataaa aacgagcagg caaaagtgga ccaaatggtc
    30301 aaaatttaca ttaagacaac aaaaatagaa gacaaaacat gtaatttttg gcagttatag
    30361 aaaaattaaa agcataacac caaaaat
//
LOCUS       DAZFAB010000049        30060 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZFAB010000049 DAZFAB010000000
VERSION     DAZFAB010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.049"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(240..2069)
                     /gene="glmS"
                     /locus_tag="ACHJWA_002531"
     CDS             complement(240..2069)
                     /gene="glmS"
                     /locus_tag="ACHJWA_002531"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFO4309109.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFAEQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2231..3601)
                     /gene="glmU"
                     /locus_tag="ACHJWA_002532"
     CDS             complement(2231..3601)
                     /gene="glmU"
                     /locus_tag="ACHJWA_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFO4309110.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3953..4372)
                     /gene="atpC"
                     /locus_tag="ACHJWA_002533"
     CDS             complement(3953..4372)
                     /gene="atpC"
                     /locus_tag="ACHJWA_002533"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFO4309111.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4393..5775)
                     /gene="atpD"
                     /locus_tag="ACHJWA_002534"
     CDS             complement(4393..5775)
                     /gene="atpD"
                     /locus_tag="ACHJWA_002534"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFO4309112.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5802..6665)
                     /gene="atpG"
                     /locus_tag="ACHJWA_002535"
     CDS             complement(5802..6665)
                     /gene="atpG"
                     /locus_tag="ACHJWA_002535"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFO4309113.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6716..8257)
                     /gene="atpA"
                     /locus_tag="ACHJWA_002536"
     CDS             complement(6716..8257)
                     /gene="atpA"
                     /locus_tag="ACHJWA_002536"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFO4309114.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8270..8803)
                     /gene="atpH"
                     /locus_tag="ACHJWA_002537"
     CDS             complement(8270..8803)
                     /gene="atpH"
                     /locus_tag="ACHJWA_002537"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFO4309115.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8818..9288)
                     /gene="atpF"
                     /locus_tag="ACHJWA_002538"
     CDS             complement(8818..9288)
                     /gene="atpF"
                     /locus_tag="ACHJWA_002538"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFO4309116.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9350..9589)
                     /gene="atpE"
                     /locus_tag="ACHJWA_002539"
     CDS             complement(9350..9589)
                     /gene="atpE"
                     /locus_tag="ACHJWA_002539"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFO4309117.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9636..10451)
                     /gene="atpB"
                     /locus_tag="ACHJWA_002540"
     CDS             complement(9636..10451)
                     /gene="atpB"
                     /locus_tag="ACHJWA_002540"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFO4309118.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10460..10840)
                     /gene="atpI"
                     /locus_tag="ACHJWA_002541"
     CDS             complement(10460..10840)
                     /gene="atpI"
                     /locus_tag="ACHJWA_002541"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFO4309119.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(11457..12080)
                     /gene="rsmG"
                     /locus_tag="ACHJWA_002542"
     CDS             complement(11457..12080)
                     /gene="rsmG"
                     /locus_tag="ACHJWA_002542"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFO4309120.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(12144..14033)
                     /gene="mnmG"
                     /locus_tag="ACHJWA_002543"
     CDS             complement(12144..14033)
                     /gene="mnmG"
                     /locus_tag="ACHJWA_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFO4309121.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSDDKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(14412..14855)
                     /gene="mioC"
                     /locus_tag="ACHJWA_002544"
     CDS             complement(14412..14855)
                     /gene="mioC"
                     /locus_tag="ACHJWA_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFO4309122.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(14945..15403)
                     /gene="asnC"
                     /locus_tag="ACHJWA_002545"
     CDS             complement(14945..15403)
                     /gene="asnC"
                     /locus_tag="ACHJWA_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFO4309123.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15555..16547
                     /gene="asnA"
                     /locus_tag="ACHJWA_002546"
     CDS             15555..16547
                     /gene="asnA"
                     /locus_tag="ACHJWA_002546"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFO4309124.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(16552..18003)
                     /gene="viaA"
                     /locus_tag="ACHJWA_002547"
     CDS             complement(16552..18003)
                     /gene="viaA"
                     /locus_tag="ACHJWA_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFO4309125.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(17997..19493)
                     /gene="ravA"
                     /locus_tag="ACHJWA_002548"
     CDS             complement(17997..19493)
                     /gene="ravA"
                     /locus_tag="ACHJWA_002548"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFO4309126.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPASLQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHNMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQRCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            19716..21584
                     /gene="kup"
                     /locus_tag="ACHJWA_002549"
     CDS             19716..21584
                     /gene="kup"
                     /locus_tag="ACHJWA_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFO4309127.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANQEVLYALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            21751..22170
                     /gene="rbsD"
                     /locus_tag="ACHJWA_002550"
     CDS             21751..22170
                     /gene="rbsD"
                     /locus_tag="ACHJWA_002550"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFO4309128.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKHHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            22178..23683
                     /gene="rbsA"
                     /locus_tag="ACHJWA_002551"
     CDS             22178..23683
                     /gene="rbsA"
                     /locus_tag="ACHJWA_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFO4309129.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFESCDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            23688..24653
                     /gene="rbsC"
                     /locus_tag="ACHJWA_002552"
     CDS             23688..24653
                     /gene="rbsC"
                     /locus_tag="ACHJWA_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFO4309130.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            24678..25568
                     /gene="rbsB"
                     /locus_tag="ACHJWA_002553"
     CDS             24678..25568
                     /gene="rbsB"
                     /locus_tag="ACHJWA_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HFO4309131.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            25694..26623
                     /gene="rbsK"
                     /locus_tag="ACHJWA_002554"
     CDS             25694..26623
                     /gene="rbsK"
                     /locus_tag="ACHJWA_002554"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFO4309132.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            26627..27619
                     /gene="rbsR"
                     /locus_tag="ACHJWA_002555"
     CDS             26627..27619
                     /gene="rbsR"
                     /locus_tag="ACHJWA_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFO4309133.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(27585..29012)
                     /gene="mdtD"
                     /locus_tag="ACHJWA_002556"
     CDS             complement(27585..29012)
                     /gene="mdtD"
                     /locus_tag="ACHJWA_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFO4309134.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPILGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNHVPSESE"
     gene            complement(29035..29727)
                     /gene="yieP"
                     /locus_tag="ACHJWA_002557"
     CDS             complement(29035..29727)
                     /gene="yieP"
                     /locus_tag="ACHJWA_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFO4309135.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         7259 a         7691 c         7674 g         7436 t
ORIGIN      
        1 gattgtttta caagcgattc ttgaatgaac aaaacgtgct attcattaca aagtacatca
       61 attatggcaa ttgtttgtca atgattcgag gtatttaatt actactacgg ttaaaacaag
      121 tgttcaacgg tgccccaaat aatagctgaa gttaattata tggaatgagg ttagattatt
      181 aatgctggta agtaaaaggg ccgccaaaag gcagcccttc gttcagcaaa tgaataatat
      241 tactcaaccg ttaccgattt tgccaggtta cgcggctggt caacgtcggt gcctttgatc
      301 agcgcgacgt gataagccag taactgcagc ggaacggtgt agaagatcgg tgcaatcacc
      361 tcttccacat gcggcatctc gatgatgtgc atgttatcgc tacttacaaa acccgcatcc
      421 tgctcggcga agacatacaa ttggccgcca cgcgcgcgaa cttcttcaat gttggatttc
      481 agtttttcca gcaattcgtt gttcggcgca acgacgataa ccggcatatc ggcgtcaatc
      541 agcgccagcg gaccgtgttt cagttcgcct gcagcgtagg cttcagcgtg aatataagag
      601 atctctttca gcttcaatgc gccttccagc gcgattgggt actgatcgcc acggcccagg
      661 aacagcgcgt gatgtttgtc agagaaatct tctgccagag cttcaatgcg tttgtcctga
      721 gacagcatct gctcaatacg gctcggcagc gcctgcagac catgcacaat gtcatgttca
      781 atggaggcat ccagaccttt caggcgagac agcttcgcca ccagcatcaa cagcacagtt
      841 aactgagtgg tgaatgcttt agtggatgcc acgccgattt ccgtacccgc gttggtcatc
      901 agcgccagat cggattcgcg caccagagaa gaacccggaa cgttacagat tgccagcgaa
      961 ccaaggtaac ccagctcttt cgacagacgc aggcccgcca gggtatccgc ggtttcgcca
     1021 gactgtgaca aggtgatcat caggctgtta cgacgcacgg cagatttacg atagcggaat
     1081 tcagaggcga tttcgacgtc gcacggaata cctgccagcg attcaaacca gtaacgggaa
     1141 accataccgg agttataaga agtaccacag gcgaggatct gaatatgctc aaccttcgac
     1201 agcagttcgt cggcgttcgg tcccagctcg cttaaatcaa cctgaccgtg gctgatgcgc
     1261 ccggtaaggg tgtttttgat cgcgttcggc tgttcgtaga tctctttctg catgtagtga
     1321 cggtaaatac ctttatcgcc cgcgtcatat tgcagattgg attcgatatc ctgacgtttt
     1381 acttccgcgc cagttttatc gaagatgttt accgaacggc gagtgatttc cgcaatatcg
     1441 ccctcttcaa ggaagataaa gcgacgggtc accggcagca gcgccagctg gtcagaagcg
     1501 ataaagtttt cacccatacc cagaccaatc accagcggac taccagaacg tgctgccagc
     1561 agggtatccg ggtgacggga gtccatgatc actgtaccgt acgcaccacg cagttgcggg
     1621 atagcacgca gaacagcctc acgcagagta ccgccctgtt tcagctccca gttcaccaga
     1681 tgggcaatca cttcggtgtc ggtttcagaa acgaaggtat agccacgcgc ttttaactct
     1741 tcgcgcagcg gttcatggtt ttcgatgatg ccgttatgca ccaccacaat gtgttcagaa
     1801 acatgcggat gcgcattcac ttctgaaggt tcaccgtggg tcgcccagcg agtgtgagca
     1861 ataccggtgc cgccatgcag aggatgttct tccgctgcct gagccagcat ctggacttta
     1921 ccgaggcgac gcaggcgggt catatgacct tccgcatcaa caacggccag accggcagag
     1981 tcatatccgc ggtattccag acgacgtaaa ccttcaagaa ggatttctgc tacatcacgt
     2041 tgcgcgatcg cgccaacaat tccacacata gtttttgatt ccgatttata tcgttgtcgg
     2101 tcaacctgta tgcccgtttt ttcgggcgcc ccgagccttg tagagagtgg ggttattttt
     2161 atagttactg cttgtgggag ggagattatg ttatctcctc atcccatgtg accggattac
     2221 ccggccagaa tcactttttc tttaccggac gacgccagcc ttctttctga gtctgcggca
     2281 cacggctgat agctaatgca ttttcgccga cattacgcgt cacagttgta cccgcagcaa
     2341 tggtcgcgcc tttgcctact gttaccgggg ccaccagctg agtgtcggaa ccgacaaaca
     2401 catcgtcgcc gataatggtc ttaaatttat tcgcaccatc gtagttgcag gtaatggttc
     2461 ccgcgccgat gttaacgtta tcgccaattt ccgcatcgcc aaggtaagtc agatgaccag
     2521 ctttcgagcc tttacccaga cgtgcttttt tcatctcaac aaagttaccg acgtgtgcac
     2581 cttccaacaa ctcagcacca ggacgcaaac gggcaaacgg gccaatggta caggccgccg
     2641 ccagattcgc gtcttccacg acggtatatg ggctaatttc gcaatcatcg ccaatcacgc
     2701 tgtttttaat cacgcaaccg gtgccgattt tcacgcgatg gccgagagtc acgttgccct
     2761 cgatgataac gttagtatca atttcaacat cgcgcccgtg agtaagcgta ccgcgcagat
     2821 caaaacgcgc cggatcgcgc agcataacgc ctgctaacag cagtttttca gcctgttcgg
     2881 actggtaaac gcgctccaga cgggagagtt gcaggcggtt attcacgcct tctacttcgc
     2941 ttaaacgttg cggatgaacg gcgacgattt cacgcccttc ctgatacgcc agcgcaataa
     3001 tgtcggtgat gtagtattcg ccctgcgcat tattgttggt cagcttcgcc agccagcgtt
     3061 tcatatctgc gccgttagcg atcagaatgc cggtgttgat ctcctgaatc tgacgctgct
     3121 cgtcggtggc atctttgtgc tcaacaatgc cggtaacttt gccgttttca cgggtgatac
     3181 gtccataacc ggtcggatca tccagtttca ccgtcagcag accaatgcca ccctgcggtt
     3241 tagcatcacg cagacgctgg agtgtttcga cagagatcag cggcacgtcg ccgtagagca
     3301 ttaaaatgtc ttcatcatcg gcaaagaaag gtgcggcctg ctgcatggca tgacccgtac
     3361 ccagctgctc tgcctgaagc acccagttca ggttgtcgtc tttcagcgcc tgttttagca
     3421 gatcgccacc gtgaccgtac accaggtgaa cgtgcgctgc gcctaattca ttcgcagcat
     3481 caatgacatg ctgaaccatc gctttcccgg caagggtatg cagcactttc ggaagatcgg
     3541 aatacatgcg cgtgccttta cctgcggcaa ggatcactac gctcatagca ttattcaaca
     3601 tacgcgtcct gactgtaatt tgagaacgaa tttaaaccgc ttcaccttga aaaaactaca
     3661 tttttttcat cgtgaaatgg acagaggata aattgttcaa taacggatta tccccgctga
     3721 cgaaagcgcc attttcgacc atcgtcacgc cttttgtctg cagaaaatta aggcagtacc
     3781 ttcatttaag cgtcaggtgg atgtttttgc tcttatttcg atcaatgaat aaacgggaaa
     3841 acagggggtt ttatatcagc aggatctatg tgaacgctat tcaggacggg tcacacgcgc
     3901 aaaaaaaagc cagcctgttt ccagactggc ttttgtgctt ttcaagccgg tgttacatcg
     3961 cttttttggt caactcgata acgcgcagct gcgcgatcgc tttggccagt tccgcagacg
     4021 cctgagcgta atctacgtcg ccgtgagagc tgctaatgtg ctcttcagcc ttacgtttcg
     4081 cttccatggc tcgcgcttcg tcgagatcct ggccgcgaat tgcggtgtcg gccagaacgg
     4141 tcacgttgcc aggctgcact tcaagaatgc cgccagacag atagataaac tcttcgtgac
     4201 cgtgctgttt cacgatgcga atcataccag gcttaatggc ggtgagcagc ggtgcgtggc
     4261 cagggtagat ccccagttca ccttcgctac ccgttacctg gattttctcg accagaccag
     4321 agaacatttg ttgctctgcg ctgacgacgt ccaggtggta agtcattgcc atatcaccct
     4381 ccgattaagg cgttaaagtt ttttggcttt ttccacagct tcttcgatgg aaccgaccat
     4441 gtagaacgcc tgctccggca ggtgatcgta ttcgccttcc atgatgcctt taaagccacg
     4501 gatggtgtct ttcagggaga cgtatttacc cggagaaccg gtgaatactt ctgccacgaa
     4561 gaacggctgg gacaggaagc gctggatctt acgagcacgc gctaccacca gtttgtcttc
     4621 ttcagacagt tcatccatac ccaggatggc gatgatgtct ttcagttcct gataacgttg
     4681 caggatggac tgaacgccac gcgcggtgtc gtagtgttcc tgaccaacca ccagcgggtc
     4741 cagctgacgg ctggtggagt ccagcgggtc aacggccggg tagataccca gagacgcgat
     4801 ctgacggctc agtaccacgg ttgcgtcaag gtgcgcaaag gtggttgccg gagacgggtc
     4861 agtcaagtca tccgcaggta cgtatactgc ctgtacggag gtgatagaac cggttttggt
     4921 ggaggtgata cgttcctgca gaacgcccat ctcttccgcc agggtcggct gataacctac
     4981 cgctgaaggc atacggccca gcagtgcgga tacttccgta ccggccaggg tgtaacgata
     5041 gatgttgtca acgaacagca gaacgtcacg accttcgtca cggaatttct cagccatggt
     5101 cagaccggtc agagctacgc gcagacggtt tcccggcggc tcgttcatct ggccatacac
     5161 cagggatact ttatcgataa cgttggagtc ggtcatttcg tggtagaagt cgttcccctc
     5221 acgagtacgt tcacctacgc ccgcaaacac agagtaaccg gagtgctcga tcgcgatgtt
     5281 acgaatgagc tccatcatgt ttacggtttt acctacaccc gcaccaccga acagaccaac
     5341 tttaccgccc ttagcgaacg gacacatcag gtcgataact ttgataccgg tttccagcag
     5401 ttcctgagag tttgacagct cttcgtagga aggtgctgcg cggtgaatcg cccaacgctc
     5461 ttcttcaccg atctcgcctt tcatgtcgac cggttcaccc agtacgttca tgatacggcc
     5521 cagagtcgct ttacctaccg ggacttcgat cgggtgttcg aggtctttta catccagacc
     5581 gcgacgcaga ccgtcggagg aacccattgc gatggtacgc acgataccgc cgccgagctg
     5641 ctgctgaact tccagcacca gacgctcatt accattttgc acctcaagag catcgtacac
     5701 gcgcggtacg gcatcctgag ggaattcgac gtcaactacg gcgccgatta cctggacaat
     5761 ctttccagta gccatcttaa atcctctacg aaataacctg tttaaaccgc ggcggccccc
     5821 gagacgatct cggtgagttc ctgagtaatg ctggcctgac gagctttgtt gtataccaac
     5881 tgcagctctt taatcaggct gccgccattg tcggtcgcgg ctttcatcgc caccatacgg
     5941 gcggcctgct cgctggccag gttttcaacc acgccctgat aaacctgaga ttcgacataa
     6001 cgacgcagca gggtatccag caacgccttc ggatcgggtt cgtacaggta atcccaggat
     6061 ttatgtttca gatcatcatc atctgatgcc ggtaacggca gcagctggct gatggtcgga
     6121 acctgagaca tggtgttaat aaatttgttg ctgacaatgt aaagcttgtc cagacggcct
     6181 tcgtcgtagg cctgcaacat cacttttacc ggaccgatca gttcggacag ggaagggtta
     6241 tcccccatgc cggtgacctg ggcaacaaca ttgccgccca cggagttgaa gaacgacacg
     6301 cctttcgagc cgatcattgc gaggtcgcat tgaacgcctt tgtcggtcca ggtcttcatt
     6361 tccgccagca gttttttgaa caggttaatg ttcaaaccac cgcacaaacc acggtcggtc
     6421 gacaccacca ggtagcccac gcgtttaacg tcgcggtctt ccaggtaagg gtgcttatat
     6481 tccagattac cgtgtgcaag gtgaccaatc actttgcgca tggtttctgc ataaggacgg
     6541 ctggccgcca tgcgatcctg cgatttacgc attttggaag cggcgaccat ctccatcgct
     6601 ttagtgatct tttgcgtgtt ctggacgctt gcgatcttac tacgtatctc ttttgcgccg
     6661 gccatgagct tctcctcaat gccttgcggc ctgccctaag gcaagccgcc agacgttacc
     6721 aggattgggt tgctttgaag gaatcgagga tgcctttcag cttgccttcg atttcgtcgt
     6781 tgtagccacc ggtctggttg atctcttgca tcaacggagc gtgatcacgg tcgacgtaag
     6841 ccagcagagc ggcttcgaag ctgccgattt tcgacagttc aacatccgcc aggtaaccac
     6901 gttctgctgc gaacagaacc agagactgct gcgcaacgga catcggcgca tactgtttct
     6961 gtttcagcag ttcggtcact ttttgaccgt ggtcaagctg cttacgtgtt gcatcgtcaa
     7021 ggtcggatgc aaactgagag aacgctgcca gttcacgata ctgtgccaga gcggtacgga
     7081 taccaccgga cagttttttc atgatcttgg tctgtgctgc accaccaaca cgggatacgg
     7141 aaatacccgg gttaaccgca ggacgaatac cggcgttgaa caggttggtt tccaggaaga
     7201 tctgaccatc ggtaatggag attacgttgg tcggaacgaa cgcagaaacg tcacccgctt
     7261 gagtttcgat aatcggcagc gcggtcagtg aaccggtttt ccctttcact tcacctttgg
     7321 tgaaagcttc aacgtattcg gcgttaacac gcgcagcacg ctccagcaga cgagagtgga
     7381 ggtagaatac gtcgcccggg aatgcttcac gtcctggcgg acgacggagc agcagcgaga
     7441 tctgacggta agcaacagcc tgtttagaca gatcatcgta aatgatcagc gcatcttcac
     7501 cgcggtcacg gaagtattcg cccattgcgc aaccggcata cggtgccagg tattgcagtg
     7561 cagcggattc agacgcggtt gctaccacaa cgatggtgtt agccagtgcg ccgtgctctt
     7621 ccagtttacg taccacgtta gaaatggtgg acgctttctg gccgatagcg acatagatac
     7681 atttgatacc ggaatcgcgc tggttgatga tggcatcgat agccagtgcg gttttaccgg
     7741 tctgacggtc accgatgatc aattcacgct gaccacgacc gattgggatc atggagtcaa
     7801 cggctttata accggtctgt accggctgat ctacggactg acgttcgata acgcccggag
     7861 cgattgcttc tacagcagag aagccgtcgt gatccagcgg acctttaccg tcgattggtg
     7921 cacccagagt gttaaccaca cggcccagca ggccacggcc aaccggaact tccaggatac
     7981 ggccagtaca cttaactttc atgccttcgg caaggtcagc atacggaccc ataacaaccg
     8041 caccaacaga gtcgcgctcg aggttcagtg cgatagcgta acggttaccc ggcagggaga
     8101 tcatttcacc ctgcatacaa tcggccaggc cgtgaatgcg gataacaccg tcacttacag
     8161 aaacaatagt accttcgttg tgagcttcac tcacaacatt gaactgagca atgcgctgct
     8221 tgatcagttc gctgatttcg gtggaattca gttgcatgct ccagtcccct taagactgca
     8281 agacgtctgc aaggcgctca agacgaccgc gtacgctgcc atcaatgacc atatcacccg
     8341 ctcggatgat aacgcctgcc attacagact tatcgatttt gcaattcagc ttaactttgc
     8401 gtgacagacg tttttccatc gcagcagaaa ttttcgcgag ctgttgttca ctcagtgcgg
     8461 cagcggaaat gacgtctacc tcagcggtag cctcactcac ggcacgaagg tgaataaact
     8521 gctccaaaac atccgggagc gcgttaagac gaccattttc agccattacc cgaataaggt
     8581 tctgaccgtt ttcgtccagt tgctcaccac aaactgcgat aaacgactcg gcaagcgttt
     8641 ctggcgcaag cgcgccagag agaagctctg ccatttgttc gtttttggtt acctcggcgg
     8701 caaacgccag catgtcctgc cagcgttcta cactttggtg ttcgacggca aagtcaaaag
     8761 ctgctttggc gtaggggcga gctaccgtaa taaattcaga catcagcccc tccctcctta
     8821 cagttcagcg acaagtttat ccacgatgtc gctgttagca gcttcatcca cggaacgttc
     8881 gatgatcttc tcggcgccag caacagccag gatagcaact tgcttacgca gctcttcacg
     8941 ggcacgttta cgctcggctt caatttccgc ctgcgcctgg gccacgattt tagtacgttc
     9001 ctgttctgcc tcagctttcg cttcgtccag aatctgcgag cggcgtttgt tcgcctgctc
     9061 gatgattacc tgggcttccg ctttcgcttt tttcagctgg tcggtcgcgc tggcctttgc
     9121 aaggtcaagg tccttatgtg ctcgttctgc ggaagcaagg ccgtcagcaa tttctttttg
     9181 acgtttttcg atggctgcca ttaatggcgg ccatacgtac ttcatgcaga acagaacgaa
     9241 caggacaaac gcgatggcct ggccgaggat tgttgcgtta agattcacag cacaatgcct
     9301 ctatttagtt aacgttctga tattgctctt taaataaaag caacgcttac tacgcgacag
     9361 cgaacatcac gtacagaccc agacctacag cgatcatcgg gatagcatcc accagaccca
     9421 taacgataaa gaactgagta cgcagcagag gaatcagatc aggttgacgc gctgcgcctt
     9481 ccaggaattt acccccgagg atgccgatac cgatcgcagc accgattgcc gccagaccca
     9541 tcatcacagc ggcagccatg tacagcagat ccatattcag gttttccatg acagtctcca
     9601 gtttgtttca gttaaaacgt agtagtgttg gtaaattaat gttcttcaga cgccatcgac
     9661 agatagacga tcgtcagaac catgaagatg aaggcttgca gcgtaatgat caggatgtgg
     9721 aaaatggccc acggcacatt caggatccac tgtgaccacc acggcaacag accagcaatc
     9781 agaatgaaaa tcagctcacc ggcatacatg ttaccgaaca gtcgcaaacc gagtgaaact
     9841 ggtttggaca gcaggcttac cccttcaagg attaagttga caggaatgaa cgcccagtga
     9901 ttgaacggct gcagcgtcaa ctctttcgtg aagccgccga tgcctttcat tttgatgctg
     9961 tagaacagaa tcaggataaa tacgcccagt gccatagaca gcgttacgtt cacgtccgca
    10021 gacggaacca cacgcagtgc aggcagaccc agtacatgtt cagcaatgta cggcagcagg
    10081 tcgataggca gtaaatccat caggttcatc aggaataccc agacgaagat cgtcagggcc
    10141 agcggagcaa tcagcttgct tttgccatgg tacatgtctt tcacgctacc attaacaaag
    10201 ccgatcacca gctcaatcgc ggtctgaaac ttacctggca caccgctggt cgcctttttg
    10261 gctacgctac ggaataaaac caggaacaac agacccagca ccaccgagaa gaacatggag
    10321 tcaatattga ttgtccagaa ggtggctggg gggttttgtg gatccaccag cgagaatgta
    10381 cgcaggtcca gctgaaggtt attcaggtgg tgtcctatgt aatcctgcgg cgtcatattt
    10441 tctgaagcca tgatgccttt taccctttgt tgttaattac agccggtgcc agtatctgaa
    10501 ccaccagcac caaaacccac gtaacgatca gcggcaagaa taccgccttt aaaaccgcca
    10561 acgccaccac cagtaacacc aacatcgcca gaactttgaa agcttcacca aatgcgaatg
    10621 tccaggccac ccggcctttc gctggtgtat gcgcctggtg acgccaggca aatatcataa
    10681 acaaaacgtt aggcagaaag actgccaggc ccccgcttat tgcagagacg ccccagaagg
    10741 ggtctttgag gctgaacagc aatccacttg ctatcaccac cagtaactga acgagcagaa
    10801 gcttccgagc aacgtttcga ctcacgagcg acacagacat cacgtttttc actcctgctc
    10861 ccttcgaggt atgccgcgtg tcgtataaaa ctttctttaa ggcttagagt caagcatcaa
    10921 aaagcggtca aattatacgg tgcgcccccg tgatttcaaa caataagtag ccaaaaggtg
    10981 aataaatgtt taaatatttt tccagtgcat acaattgcga cttttctact aaccctgttc
    11041 gatcatgaaa aactgtaaat aacgcgtaaa cactggtgat aaagcgtgct tcagatcaca
    11101 tattgcgcat gttcgcgcac agcatattta tttacttggc aaatgatgcc tttgcaagtt
    11161 tatgatattt cagtctaaaa acagatactg ttttaataaa tgacatttac acaacaaaaa
    11221 ccacccattg acatttttaa taatgtttta acagccaatg atggttctta gcgccgattt
    11281 ttagcagact gatattttca ctaatgactt attttctgct taccaaaaaa agccacgtta
    11341 tcttgttgat gcaaaagagt gaacgtggcg ttaaatgtaa ccagttatat cagtagaaaa
    11401 cctggttgtt gttaacagtc taaccggtca attttttatg atttttttga taaaaattaa
    11461 attttatttg ctttaatcac caccagatga cgttcgccat ccagggctgg aacctgaagt
    11521 ttaaccactg attcgacctg atattcttcg ggcaacaaag cgatttcatc ttccggcatt
    11581 tgccctttca gcgcgtagaa acggccttgc tcaccaggaa gatggtggca ccagctcacc
    11641 atatcgttca gagaggcaaa agcgcggcta attacgccat caaatggcgg ctctgaagga
    11701 aactcttcta ccctgctctg tactggctca atattctcca gtttaagctc atgttgcacc
    11761 tgacgaagga aacgcacgcg tttaccaagg ctatccaaca gagtgaaatg ggcttcagga
    11821 cgcacgatag agagtggaat gccaggcagt ccaggaccgg tgccgacatc gataaaccgt
    11881 tcaccttgca gatacggtgc caccacaatg ctatcgagaa tatggcgtac cagcatctca
    11941 ttaggatcgc ggaccgaagt caggttgtac gctttgttcc atttatgcag catattcacg
    12001 taggcaataa gctggttttt ctggtgatcg gtaagcgaaa tacctgcgtc tttcagcagt
    12061 aaggagagtt tgttgagcac ggtgattacc tgttcttgat gcgttgcctg gtaagcgggg
    12121 gcttaccagg catttttaat gcgttatgcg ctacgacgca gcataccctg ttttttcagc
    12181 cacaccagca gaatggagat ggccgcaggc gtgacgccag aaatacgtga agcctggccg
    12241 atagaggccg gtttgtgatc gttaagtttg gcgatcactt cgttagaaag accggatacc
    12301 tggcggtaat ccagtgtcgc gggtagcaag gtgttctcgt tacgcagctg cttttcgatc
    12361 tcatcttgct ggcgcgcgat ataaccttcg tatttaacct gaatctcaac ctgttccgcc
    12421 gcctgttcgt ctgtcaacgc aggggcaaac ggcgtcagcg tggttaattt ttcataagtc
    12481 atttccggac gacgcagcag atcttcacca ctggcttcac gggaaagcgg cgcagtcagg
    12541 tgagcattca cttcggctgc agcttccgcc gacggggtta cccaggtcga tttcagacgc
    12601 tgacgctcac gctcgatatt ctcaagtttc tcgttaaagc gcgcccaacg ttcgtcatcc
    12661 accaggccca gttcacgacc gatttcagtc aaacgcagat ccgcattatc ttcgcgcagc
    12721 atcagacgat attctgcgcg cgaggtaaac atacgatacg gttctttggt tcctaaagtg
    12781 cacaggtcat caaccagtac gccgagatag gcctgagaac gcgccggagc ccaaccttct
    12841 ttgtcatcag acagacgggc agcgttaaga ccggccagca aaccttgcgc agcggcttct
    12901 tcgtaaccgg tagtgccgtt aatctgacca gcaaagaaca gcccctggat aaacttgctc
    12961 tccagcgtcg gtttcaggtc gcgaggatcg aagaagtcat actcaatggc ataacccgga
    13021 cgcacgatct tcgcgttttc catcccctgc atagagcgga cgatttgcat ctgcacatcg
    13081 aacggcaggc tggtggagat accgttcgga taaatttcgt tagaagtcag cccttccggt
    13141 tcaaggaaga tctgatgctg atttctgtcg gcgaagcgca tgactttgtc ttcgatcgac
    13201 gggcagtagc gtgggccgac accttcgatc acccctgcgt acattgggct acgatcgagg
    13261 ttactgcgga tcacatcatg ggttttctcg ttggtatggg tgatataaca cggcacctgc
    13321 tgtggatgct gggacgcatt gcccataaaa gagaataccg gcattgggtt atcgccatgc
    13381 tgttgcgcaa gcacgctaaa gtcgatggtt cgagcatcaa tacgcggtgg tgtcccggtt
    13441 ttcagacgac caacgcgcag cggaagttca cgcaaacggc gagaaagcgg aatggacggc
    13501 ggatcaccag cacggccacc gctgtagtta tccagaccga tatgaatttt accgtcgagg
    13561 aacgtcccaa cggtgagcac gacggctttg gcacggaact tcagtcccat ttgggtaaca
    13621 gcaccgacca cacgatcgtt ttcgacaata agatcttcaa ccgcctgctg gaagatcatc
    13681 aggttcggtt ggttctccag cgccgtacgt accgcctgac ggtagagcac acgatccgcc
    13741 tgagctcggg tagcgcgaac tgccggtccc ttgcttgcgt ttagtatcct aaactggata
    13801 cccgcctgat cgatcgcttt cgccatcaga ccgccgagtg catccacttc ttttaccaga
    13861 tgtcccttcc caataccgcc gatcgccggg ttgcagctca tctgccccag agtgtcgata
    13921 ttgtgtgtca aaagcagagt ctgttgaccc atacgcgccg cagccatcgc ggcctcggtg
    13981 cctgcatgac ccccgccaat gatgatgacg tcaaaaggat ccggataaaa catggtgatt
    14041 gcctcgcata acgcggtatg aaaatggatt gaagcccggg ccgtggattc tactcaactt
    14101 tgtcggcttg agaaagacct gggatcctgg gtattaaaaa gaagatctat ttatttagag
    14161 atctgttcta ttgttatctc ttattaggat cgcactgccc tgtggataac aaggatccgg
    14221 cttttaagat caacaacctg gaaaggatca ttaactgtga atgatcggtg atcctggacc
    14281 gtataagctg ggatcagaat gaggggttat acacaactca aaaactgaac aacggttgtt
    14341 ctttggataa ctaccggttg atccaagctt cctgacagag ttatccacag tagatcgcac
    14401 gatctgtata cttatttgag taaattaatc cacgatccca gccattcttc tgccggatct
    14461 tccggaatgt cgtgatcaag aatgttgatc ttcagtgttt cgcccgtctg ttttgcaccg
    14521 gaatttttga gttcagcctc gagtttatcg atagccccac aaaatgtgtc atattcacga
    14581 ctaccaatac cgattgcgcc aaagcggacc gcagaaagat cgggcttctg ttcctgcaat
    14641 gcttcataga aaggagaaag gttgtccgga atatctccgg caccgtgggt ggagctgata
    14701 accagccaga tccccgaggc agataaatct tccaacagcg gaccgtgcag cgtttcggtg
    14761 gtaaaacccg cctcttccag cttttcagcc aggtgttctg ctacatattc ggcaccgccg
    14821 agggtgctgc cgctgataag agttatatct gccataaacc gccaccttta ttaagagtgg
    14881 cgtattgtac gctgtgaacg cgttgggatc tacctgtgga aaagtatggg attaaaaaag
    14941 ccgatcaggg cttgatggta cgcatgatcg ggttctgcag gacgatcagt gtctcggtgg
    15001 actgaatttc atcaattgtt tggatcttgt tgataagtac atgctggaga gcatcgatcg
    15061 aacggcacat cacttttata aagatgctgt agtggccggt ggtgtagtac gcctcggtca
    15121 cttcatcgag gctttccagc tttgccagcg cggaagggta gtctttggcg ctctttaata
    15181 taatgccgat aaagcagcct acgtcataac cgagctgctt cgggctgaca tcaatacgcg
    15241 ccccggtaat gatccccgcc tgcttcattt tctctactcg aacgtgaatc gtccccggac
    15301 tgacgccaaa ttgtttcgcc agttcggcgt aagcggtgcg cgcattgccc attaatgctt
    15361 ccaggatgcc acggtccaga ttgtcgatca gataattttc cataggattt tcttatgcgg
    15421 attgatgatt cattctattt tagccttctt ttttaatgaa tcaaaagtga gttaggcttt
    15481 ttattgaatg attattgcat gtgtgtcggt ttttgttgct taatcataag caacaggacg
    15541 caggagtata aaaaatgaaa accgcttaca ttgccaaaca acgccaaatt agcttcgtga
    15601 aatctcactt ttctcgtcaa ctggaagaac gtctggggct gatcgaagtc caggcgccga
    15661 ttcttagccg tgtgggggat ggcacgcagg ataacttgtc gggctgtgaa aaagcggtac
    15721 aggtaaaagt gaaagctctg cctgatgccc agttcgaagt ggttcattca ctggcgaagt
    15781 ggaaacgtca gaccttaggg caacacgact tcagcgcggg cgaagggctg tacacgcaca
    15841 tgaaagccct tcgccccgat gaagaccgtc tttctccgtt gcactcggtc tatgttgacc
    15901 agtgggactg ggaacgcgta atgggcgacg gtgagcgtca attctcgact ctgaaaagca
    15961 cggtagaggc gatttgggcg ggaattaaag caaccgaagc tgcggttagc gaagagtttg
    16021 gcctggcacc gttcctgccg gatcagatcc acttcgtaca cagccaggag ttactgtctc
    16081 gttatccgga tcttgatgcc aaagggcgtg agcgggcgat agcgaaagat cttggtgcgg
    16141 tattccttgt cgggattggc ggcaagctga gcgatggtca tcgccacgac gtgcgcgcac
    16201 cggattatga tgactggagc accccgtcag agctgggcca tgcgggtctg aacggcgata
    16261 ttctggtgtg gaaccctgta ctggaagatg cgtttgagct ttcttccatg gggatccgcg
    16321 tggatgccga cacgttgaag catcagttgg cgctgaccgg tgatgaagat cgtctgcagc
    16381 tggaatggca tcaggcgttg ctgcgcggtg aaatgccgca gaccatcggc ggcggtatcg
    16441 gccagtctcg tttgaccatg ctgctgctgc aactgccgca tatcggccag gtccagtgtg
    16501 gagtatggcc agctgctgtt cgcgagagcg tcccttctct gctgtaataa tttatcgccg
    16561 ccagcgtctg agcaggcggc ttcgcatccc ggtatcaaag cgccagatat gatcgaaaat
    16621 gcgcatgatg ccgggtttgc cgtgtgccga catcgccacg gcatgaaagc gatgctgatg
    16681 tacccgctgc agctctttca ctttactcgt cacgtcgtca ggcaaccgct gggcgataaa
    16741 atcagaaatc accaccgcat cggcatcaaa ccattccctg ctttgcaagc gttccataat
    16801 ggcgcgaaaa caactggcaa gatcggtgcc gccacgaaac tgctggctta aaaaacggat
    16861 tgcttgttcg atgccttgtg ggcctgaaag ctcataacgg acgatctcgg tggaaaatag
    16921 cataatataa cagcgccggt tttcagcgag agcaatgcgc atcaaagcca ggcagaacgc
    16981 tttcgcacac tgttcattaa agccgcccat tgagccggaa gtatccacgc agacaataaa
    17041 cggtccgcgc ggctgttcgt cgtaatcttt atgcaccacc gggcgttcga tcactttttc
    17101 acgccacgac tcaccgtgca ggcgataggt gagcaactgt ttttccacca gccgacggta
    17161 aaactcatac tccagttccg ttatccctag tgtcgccagt tctggcggca gaagacgtaa
    17221 aatatcatcg ctttgttgca gaccatcaac ctgctcagga accgtcgccg gttcgcgcac
    17281 catggtgcgg aaggtttcca tctgcgcatc gttgcgcggt attgatttgg cttcccggga
    17341 acgccccagt tgttctgcca ggcgtttcag ttccggctgt tcgttaagaa attcaccgta
    17401 tttcacaatc aactgatagt cgccacgttt aagctgaccg gcgctcatat cccacagacg
    17461 accagctgcg gtattgttat ctgcgagaat cggttcaagt tgtccgctca gcgtcatgcg
    17521 ttcctgaact tcactcaaca gttgttcgcg ttcttcttct aatagctgtt gatttaacgt
    17581 tgttgcttgc acgatcagac ttaaacgcca gcgctggaga aaaagcgtgt gtaacgctga
    17641 agtgatcgtg ctgttagcat cgaccaactg tcgggcttgt tctgcccatg gggaattcag
    17701 acgatgcagt aagtctagga tctgtggtag ctgcacaata aactgcggcg tggagaggag
    17761 ctggctttgc tgatagcaca tcacctcttc ggtgagttcc ggcgggactc gggcatcttt
    17821 cagccgactg cgcagcgcct cacgccagcg gggaacatca tcagtgattg ccgccttcag
    17881 tcgtgggaat ttttcaaaga agactgccag ctgcggtgag gccagcagcg cgatgatcat
    17941 ctcttcgatc aatccctctt cgctgacggc cagcatcaca ttaagcgtat ccagcgttag
    18001 cattgttgtg cctggcgaat ctgtgcgccg acatcctgta ggctggcttc aatgcgacct
    18061 aaccagtcgc cagggataaa cagacatctt tgctgttcgc tgaataaagc gtgttgcttg
    18121 cgccagtcgc tttcaagctc ttccaattgt tgttttattt cacctggcag accttcagcc
    18181 gatgaaccgg gaagtgccag cgtactgcct tgtaaactta catcgcgtac aacaagatgt
    18241 tgggcgctat caacttccag attcagtttc tgggcaaagc cgataccgtt cagtttgccg
    18301 cgaatttcac cacctttgct cagccactgt tccagcgcgc tacgctcaaa ggagatatga
    18361 accacttcca tattatgcaa ttttaacggt ttttgcagca gcagagtcag agtggaagca
    18421 gtaacgttaa caggaagttg atactgctga cgacggctga aaatgccgcc cagacgaatt
    18481 accgttaagg ctgttttatc gctttgttgc tgctggagtt gcaggtgacg ttgcacaatc
    18541 gcgcccaggc gggtcaacat cccttgctgt tgccaggcat gaccggtcat caatacatca
    18601 atttgttgtt gtatcaaatt caggctttgc gcgtcatacc acaggcaatc tttcagcaaa
    18661 atgagatcta ccggggcaac agcactgcga ccgctaaaaa aggcgctggc ctgcaataat
    18721 cgaatcgctt ttttccagcg acgatccgag acataaggcg catccggtaa tttatccagt
    18781 tgctggcgca gcataaaaat cagctcaaat acatgatcgg gcagcgtaat ttcaccaatc
    18841 tctttttgcc agcgttcata ttcttcatct gtgacctgca aggaggctgg aaccggattg
    18901 tcattttcat cctgttgact ggtcagcatg gagcggaaat tcgctttatc ctgcacttta
    18961 tctaaccaca gacgaatcag catgcggtca tataacgctt ccagactgct gtctgcttcc
    19021 ggcagctcgt tggaggccgc caccagcagg cgcatcggga ttttttctac gtgtgcaccg
    19081 ttgcggaact ggcgctcgtt aatggcggtg agcagggtat taagaattgc cggacccgct
    19141 ttccagatct catccagaaa gacgatttct gcttccggca ggtaaccgct ggttaaacgt
    19201 tcatagcgcc cttcatcttt tagcgcctga atagaaaggg gaccaaaaac ttcttccggc
    19261 gtggagaagc gggtcatcag atattcaaac gcgcgggcat tctgaaaggc gaattttaag
    19321 cgccgggcga tcaaactttt ggcaatacct ggcgggccaa ggaggaacac actttcacca
    19381 cttaatgccg ctaataaaca caagcggatg gcgtggctac gttcataaag ccccttttcc
    19441 aacgaactgc tcaggcggga aattctttcc gctaataaat gagggtgagc cataatgaag
    19501 tggcgtcctt tcgtcaaaag ttctgcgtaa attgcgagta tagacgtttc ttgctggtgg
    19561 ctaaaatagt ctcaaagggg gggtattttt ctttgagcca ggttaatgtg gccgcattta
    19621 ggagtacgat tttgccgtta atcgtgcata ctgtgcgctt ttttgtgggc caagggacta
    19681 agcacacatt tcatatttca acgaaagact agtctatgag cactgataat aagcaatcat
    19741 tgcccgcgat taccctcgcg gcgattggag ttgtctacgg cgatattggt accagcccgt
    19801 tatatacact tcgtgaatgt ttgtccggcc agtttggttt tggcgttgaa cgcgatgccg
    19861 tgtttggctt tttatcgctg atcttctggc tgctaatctt tgtggtttcc attaaatatc
    19921 tcaccttcgt gatgcgggca gataacgccg gtgaaggggg gatcctgacg ttgatgtcgc
    19981 ttgccgggcg taatacgtcg gcgcgaacca catcaatgct ggtgattatg gggctaatcg
    20041 gcggcagctt tttctatggt gaagtcgtca taacacccgc tatttcggtg atgtcagcca
    20101 ttgaaggtct ggaaatcgtc gccccgcagc tggatacctg gatagttccc ctctcaatta
    20161 tcgttctcac tttattattt atgattcaaa agcatggcac ggctatggtc ggtaagctgt
    20221 ttgcgccgat catgctgacc tggtttttga ttctggcagg actggggtta cgtagcatta
    20281 ttgctaacca ggaagtgctg tatgcgctga atccgatgtg ggcggtgcat ttcttccttg
    20341 aatacaaaac ggtttctttt attgcattag gggcagtggt gctgtcgatt acgggggtcg
    20401 aggcgctgta tgctgatatg gggcactttg gtaagttccc tattcgcctg gcgtggttca
    20461 ccgtcgtatt accttccttg acccttaatt acttcggtca gggagcgctg ttgttaaaga
    20521 atccggaagc gattaagaac ccgttcttcc ttttggcacc ggactgggcg ctgatcccgc
    20581 tgctgatcat cgccgcactg gcgacggtaa tcgcctcgca ggcggttatc tctggcgtct
    20641 tttcattgac gcgtcaggcg gtacgtctgg gatatttgtc gccgatgcgc attattcaca
    20701 cctccgaaat ggagtcaggg caaatctata ttccgtttgt gaactggatg ctctatgtcg
    20761 cggtcgtgat tgtgattgtc agctttgagc actccagcaa cctggcggcg gcgtacggga
    20821 ttgcggtgac cggaaccatg gtgctgacgt ctattctctc gactaccgtg gcacgtcaga
    20881 actggcactg gaataagtat tttgttgcgc tgatcttgat tgctttcctt tgtgtcgata
    20941 ttccattgtt caccgctaac ctcgataaac tgctctccgg cggctggttg ccattgagcc
    21001 tcggtacggt gatgtttatc gtgatgacca cctggaagag tgagcgtttc cgcttgctgc
    21061 ggcggatgca tgaacatggt aactctctgg aagcgatgat tgcttcgctg gagaaatcac
    21121 cgcctgttcg cgtgcccggg accgcggtat atatgtcgcg tgcaatcaac gtcattccct
    21181 ttgcgctgat gcataacctt aaacataaca aggtattgca tgagcgggtg attctgttaa
    21241 ctctgcgcac cgaagacgct ccatatgtcc ataacgtccg tcgggtacag attgaacaac
    21301 tgtcgcccac tttctggcgc gtggtggcaa gttatggttg gcgagaaacg ccaaacgtgg
    21361 aagaagtttt ccaccgctgc ggtctggaag gattaagttg ccggatgatg gaaacctcct
    21421 tctttatgtc gcatgagtcg ttgatcctcg gcaaacgccc gtggtatttg cgtctgcgcg
    21481 gcaagctgta cttgctgctg caacgtaatg cgctgcgtgc accagatcaa tttgaaatcc
    21541 cgccaaacag ggttatcgaa ctgggtactc aggtcgaaat ctaacgccag acgcctcctt
    21601 tcttcataag ggggcgtttt tgttttcatg gttaatcacc atgtaaaacg tttcgaggtt
    21661 gatcacattt ccgtaacgtc acgatggttt tcccaactca gtcaggatta aactgcgggt
    21721 cagcgaaacg tttcgctgat ggagaaaaaa atgaaaaaag gcaccgttct taattctgat
    21781 atttcatcgg tgatctcccg tctgggacat accgatacgc tggtggtgtg tgatgctggt
    21841 ttacccatcc ccaaaagtac aacgcgtatc gatatggcat taacccaggg tgtaccttct
    21901 tttatgcagg tgctgggcgt cgtcacaaat gaaatgcagg tcgaggctgc cattatcgcg
    21961 gaagagatca aacaccataa tccgcaactc cacgaaacgt tgctcactca ccttgagcag
    22021 ctgcaaaaac accagggaaa taccattgaa attcgttaca ccacgcatga acaattcaaa
    22081 caacaaaccg cagaaagtca ggcggtaatt cgcagcggag aatgttctcc gtatgcgaat
    22141 atcattctct gtgctggcgt gacgttctga ggccgtcatg gaagcattac ttcagcttaa
    22201 aggcatcgat aaagccttcc cgggcgtaaa agccctctcg ggcgcagcgt taaatgtcta
    22261 tccgggccgc gtgatggcgc tggtgggcga aaacggcgcg ggtaaatcca ccatgatgaa
    22321 agtgcttact ggcatctata ctcgcgatgc cggtacgctt ttatggctgg ggaaagaaac
    22381 gacatttacc gggccgaaat cttcccagga agccgggatt gggattatcc atcaggaact
    22441 gaacctgatc ccgcagttaa ccattgccga aaacattttc ctcggtcgtg agtttgttaa
    22501 tcgctttggc aaaattgact ggaaaaccat gtatgccgaa gcggataaat tgctggctaa
    22561 acttaacctg cgctttaaaa gcgacaagct ggtgggcgat ctttccatcg gtgaccagca
    22621 aatggttgaa atagccaaag tgctgagctt tgagtcgaaa gtcatcatta tggatgaacc
    22681 gaccgatgcg ctgaccgata ccgaaaccga atccctgttc cgcgtcatcc gcgagctgaa
    22741 atcgcaaggc cgcggtattg tctatatctc ccaccgcatg aaagaaatct tcgagagttg
    22801 cgatgacgtt accgtttttc gtgacggaca atttattgct gagcgcgaag tggcatcact
    22861 gaccgaagat tcgctgattg agatgatggt gggtcgcaag ctggaagatc aatatccgca
    22921 cctggacaaa gcgccgggag atatccgcct gaaagtcgat aatctctgcg gacctggcgt
    22981 taacgatgtc tcttttactt tacgcaaagg cgaaattctt ggcgtctctg gtttgatggg
    23041 cgcaggtcgt accgaactga tgaaagtgct ctacggcgca ctaccgcgca ccagcggtta
    23101 cgtcaccctg gatgggcatg aagtcgttac ccgttcaccg caggatggcc tggcaaacgg
    23161 cattgtgtat atctccgaag accgtaaacg tgacggttta gtgttgggca tgtcagtaaa
    23221 agagaacatg tcgctgacag cgctgcgcta cttcagccgc gctggcggca gtttgaagca
    23281 tgccgatgaa cagcaggctg tgagtgattt tattcgcctg ttcaatgtga aaacgccgtc
    23341 gatggagcag gcaattggtc tgctttccgg tggcaatcag caaaaagtgg cgattgcccg
    23401 cggtctgatg acacgcccca aagtgttgat ccttgatgaa cctacccgtg gcgtagatgt
    23461 cggcgcgaaa aaagagatct atcaactgat taaccagttc aaagccgatg gcttgagcat
    23521 cattctggtg tcatcggaga tgccagaagt attaggcatg agcgatcgca tcatcgtcat
    23581 gcatgaaggg catctcagcg gggaatttac tcgtgagcag gccacccagg aagtgttaat
    23641 ggctgccgct gtgggcaagc ttaatcgcgt gaatcaggag taaaaaaatg acaacccaga
    23701 ctgtctctgg tcgccgttat ttcacgaaag cgtggctgat ggagcagaaa tcgcttatcg
    23761 ctctgctggt gctgatcgcg attgtctcga cgttaagccc gaactttttc accatcaata
    23821 acttattcaa tattctccag caaacctcag tgaacgccat tatggcggtc gggatgacgc
    23881 tggtgatcct gacgtcgggc atcgacttgt cggtaggttc tctgttggcg ctaaccggcg
    23941 cagttgctgc atctatcgtc ggcattgaag tcaatgcgct ggtggctgtc gctgccgctc
    24001 tcgcgttagg tgccgcaatt ggtgcggtaa ccggagtgat tgtagcgaaa ggtcgcgtcc
    24061 aggcgtttat cgctacgctg gttatgatgc ttttactgcg cggcgtgacc atggtttata
    24121 ccaacggtag cccagtgaat accggcttta ctgagaacgc cgatctgttt ggctggtttg
    24181 gtattggtcg tccgctgggc gtaccgacgc cagtctggat catggggatt gtcttcctcg
    24241 cggcctggta catgctgcat cacacgcgtc tggggcgtta catctacgcg ctgggcggca
    24301 acgaagcggc aacgcgtctt tctggtatca acgtcaataa aatcaaaatc atcgtctatt
    24361 ctctttgtgg tctgctggca tcactggccg ggatcattga agtggcgcgt ctctcctccg
    24421 cacaacccac ggcggggact ggctatgagc tggatgctat tgctgcggtg gttctgggcg
    24481 gtacgagtct ggcgggcgga aaaggtcgca ttgttgggac gttgatcggc gcattaattc
    24541 ttggcttcct taataatgga ttgaatttgt taggtgtttc ctcctattac cagatgatcg
    24601 tcaaagcggt ggtgattttg ctggcggtgc tggtagacaa caaaaagcag taataacgac
    24661 tacaggacat cttgaatatg aacatgaaaa aactggctac cctggtttcc gctgttgcgc
    24721 taagcgccac cgtcagtgcg aatgcgatgg caaaagacac catcgcgctg gtggtctcca
    24781 cgcttaacaa cccgttcttt gtatcgctga aagatggcgc gcagaaagag gcggataaac
    24841 ttggctataa cctggtggtg ctggactccc agaacaaccc ggcgaaagag ctggcgaacg
    24901 tgcaggactt aaccgttcgc ggcaccaaaa ttctgctgat taacccgacc gactccgacg
    24961 cagtaggtaa tgctgtgaag atggctaacc aggcgaacat cccggttatc actcttgacc
    25021 gccaggcaac gaaaggtgaa gtggtgagcc acattgcttc tgataacgta ctgggtggca
    25081 aaatcgccgg tgattacatc gcgaagaaag cgggtgaagg tgccaaagtt atcgagctgc
    25141 aaggcattgc tggtacatcc gcagcccgtg aacgtggcga aggcttccag caggccgttg
    25201 ctgctcacaa gtttaatgtt cttgccagcc agccagcaga ttttgatcgc accaaaggtt
    25261 tgaacgtaat gcagaacctg ttgaccgctc atccggatgt tcaggctgta ttcgcgcaga
    25321 atgatgaaat ggcgctgggc gcgctgcgcg cactgcaaac tgccggtaaa tcggatgtga
    25381 tggtcgtcgg atttgacggt acaccggatg gcgaaaaagc ggtgaatgat ggcaaactag
    25441 cagcgactat cgctcagcta cccgatcaga ttggcgcgaa aggcgttgaa accgcagata
    25501 aagtgctgaa aggcgagaaa gttcaggcta agtatccggt tgatctgaaa ctggttgtta
    25561 agcagtagtt ttaatcaggt tgtatgacct gatggtgaca taaatacgtc atcgacagat
    25621 gaacgtgtaa tataaagaaa agcagggcac gcgccaccct aacacggtgg cgcattttat
    25681 ggacatcccg aatatgcaaa acgcaggcag cctcgttgtt cttggcagca ttaatgctga
    25741 ccacattctt aatcttcaat cttttcctac tccaggcgaa accgtaaccg gtaaccacta
    25801 tcaggttgca tttggcggca aaggcgcgaa tcaggctgtg gctgctgggc gtagcggtgc
    25861 gaatatcgcg tttattgcct gtactggcga tgacagcatt ggcgagagcg ttcgccagca
    25921 gctcgccact gataacatcg atattactcc ggtcagcgtg atcaaaggcg aatcaacagg
    25981 tgtggcgctg atttttgtta atggcgaagg tgagaatgtc atcggtattc atgccggcgc
    26041 taatgctgcc ctttccccgg cgctggtgga agcgcaacgt gagcgtattg ccaacgcgtc
    26101 agcattatta atgcagctgg aatcaccact cgaaagtgtg atggcagcgg cgaaaatcgc
    26161 ccatcaaaat aagactatcg ttgcgcttaa cccggctccg gctcgcgaac ttcctgacga
    26221 actgctggcg ctggtggaca ttattacgcc aaacgaaacg gaagcagaaa agctcaccgg
    26281 tattcgtgtt gaaaatgatg aagatgcagc gaaggcggcg caggtactgc atgaaaaagg
    26341 tatccgtact gtactgatta ctttaggaag tcgtggtgta tgggctagcg tgaatggtga
    26401 aggtcagcgc gttcctggat tccgagtgca ggctgtcgat accattgctg ccggagatac
    26461 ctttaacggc gcgttaatca cggcgttgct ggaagaaaaa ccattgccag aggcgattcg
    26521 gtttgcccat gctgccgctg cgattgccgt aacacgtaaa ggcgcacaac cttccgtacc
    26581 gtggcgtgaa gagatcgacg catttttaga caggcagagg tgacgcttgg ctacaatgaa
    26641 agatgttgcc cgcctggcgg gcgtttctac ctcaacagtt tctcacgtta tcaataaaga
    26701 tcgcttcgtc agtgaagcga ttaccgccaa agttgaagcg gcgattaaag aactcaatta
    26761 cgcgccatca gctctggcgc gtagcctcaa actcaatcaa acacatacca ttggcatgtt
    26821 gatcactgcc agtaccaatc ctttctattc agaactggtg cgtggcgttg aacgcagctg
    26881 cttcgaacgc ggttatagtc tcgtcctttg caataccgaa ggcgatgaac agcggatgaa
    26941 tcgcaatctg gaaacgctga tgcaaaaacg cgttgatggc ttgctgttac tgtgcaccga
    27001 aacgcatcaa ccttcgcgtg aaatcatgca acgttatccg acagtgccta ctgtgatgat
    27061 ggactgggct ccgttcgatg gcgacagcga tcttattcag gataactcgt tgctgggcgg
    27121 agacttagca acgcaatatc tgatcgataa aggtcatacc cgtatcgcct gtattaccgg
    27181 cccgctggat aaaactccgg cgcgcctgcg gttggaaggt tatcgggcgg cgatgaaacg
    27241 tgcgggtctc aacattcctg atggctatga agtcactggt gattttgaat ttaacggcgg
    27301 gtttgacgct atgcgccaac tgctatcaca tccgctgcgt cctcaggccg tctttaccgg
    27361 aaatgacgct atggctgttg gcgtttacca ggcgttatat caggcagagt tacaggttcc
    27421 gcaggatatc gcggtgattg gctatgacga tatcgaactg gcaagcttta tgacgccacc
    27481 attaaccacg atccatcaac caaaagatga actgggggag ctggcgattg atgtactcat
    27541 ccatcggata acccagccga cccttcagca acaacgatta caacttactc cgattctgat
    27601 ggaacgtggt tcggcttaga tttacgctgt cttttgatca aattattacc atcggttgtt
    27661 ttcagaagca tgaacattgc tgctgaagca acagtaataa tgcccattgt gataaacgta
    27721 tagtggaatt gttcgacagt cgttgtgcct tccattcctt cataaacgcg aaggacggcc
    27781 gcacttacag ctacgcctaa actgatagac agttgctgcg tgaccgccag aacactgtta
    27841 ccgctgctgg cgttgtcatc ggtcagatcg gcaagtgtga tggtattcat cgcggtaaac
    27901 tgcgtcgaca tagccatccc taatataaac aacggcaaga tcagcatcca tattgccatt
    27961 gccggtgatt gcaaagagaa ctgagcgatc attagcccaa taatcaccgt gatccccact
    28021 aatgtatggc gatagcccag acgacgtaag acttgggtaa ccatcgattt tgcaataatg
    28081 gaacctaacg ctgtcggtgc catcatacag ccagcaataa acgcctgata accaaatcct
    28141 acctgtaaca ttaatggcat aaggaacggt acacagccgg tccccagacg ggttgcaata
    28201 ttgcctacga taccgatcga gaaagtgcgg gttttaaata aatctaatga aattaatggg
    28261 tttggcgtgc gtcgtgcatg gagaatatag agaagcagta acccgatgct ggtgacaatt
    28321 accgtcaagg caatccagct ggcgacaatc ttttccccga atagctctat tccgcttgag
    28381 aagagaacaa ggctgaggcc aaacagcaaa aagccagtga tatcgaatct gcgtcgtgcg
    28441 gtggtgaaat tgggcatatg tttgcgcgcg taaagaaggc ccgcaatacc tatggggata
    28501 ttgattaaaa atatccagtg ccaggttgcc caggtgacca gcacgccgcc aagaatgggg
    28561 cctaaaattg gccccaccag acccggcatg gcgacaaaat tcaatactgg aagaagttca
    28621 ttacgaggat aagcgcgcag taaggccagc cgagcaacag gcatcatcat tgcgccgcct
    28681 atcccctgaa taacccggaa gacaaccagc tgtggtagcg aattagaaag tgcgcaggcc
    28741 agagaaccca atgtgaacag actcacggca agggtaaaaa tgcgacgcgt accgaagcga
    28801 tcggctagcc atccgcttac cggaataagc atcgccaccg tcagcgtata actgatgatg
    28861 gctgattgca tcgcgagagg agaacgatta aggctatgag cgattgcggg taaggcggta
    28921 ttcagaatag tggcatcaag tgcctgcatg aagaaggcca tcgccgcgat ccacggcaaa
    28981 cccgccatac tgcgcttctt tttatcgctc attcaatgtc ctgttatcgg gttatcactt
    29041 atcaggtgag cgtagcagcg cctgacaagc tttaaatgcc gcgtcgccat cgctttggat
    29101 aatcgcatcg actatcgcct gatgcagatc cagctttatc actgtgtcgc tggtaattga
    29161 cgtgaagtaa gtgtgataaa ccgaatggaa tagcgaggcg aatgaggtca aaaacggatt
    29221 ggcgctcatt tcatagatat gctcatgcca ggccatatcg acttcgatcc agcgttcacg
    29281 gcgaaagttc tcttttaatg ccgccatttc ggccattaac gtattgagat gcgctttctg
    29341 ttccgcggtg ccaaccgttg ctgccagtag gcaagcttgt ggctccagac agatacgcat
    29401 aaccagaaag tgatcgatga cctgatgaaa gttctcttct gtcatccacc aggtaagcaa
    29461 ttcctgatca agaaaattcc agttcgattg tggcatgacc cgggtaccaa ttcgcggtcg
    29521 cggtaaaacc atcccttttg ccgttaacgt tttgaccgct tcgcgtaccg ctgtacgact
    29581 cactccaaat tgctcgccca gctcaatctc accgggcaaa atggtgccgg gttcatattc
    29641 accttttaag atccgttgcg ccagcttctc agccagaaca tacgaaaggt ttttctgtgc
    29701 agctaactgt tgtgcgctta aaggcattac taatcttcct ttttcttttt attcctcctt
    29761 agtatgccac caggaagtgt gattacggtt gcaaaaacgg caaattgctt gttttatggc
    29821 acattaacgg ggcttttgct gaaaaaatgc gcggtcagaa aattatttta aatttcctct
    29881 tgtcagaccg gaataactcc ctataatgcg ccaccactga cacggaacaa cggcaagcac
    29941 gccgccgggt cagcggggtt ctcctgagaa ttccggcaga gaaagcaaaa ataaatgctt
    30001 gactctgtag cgggaaagcg tattatgcac accccgcgcc gctgagaaaa agcgaagcgg
//
LOCUS       DAZFAB010000050        29018 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZFAB010000050 DAZFAB010000000
VERSION     DAZFAB010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29018
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.050"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            420..1286
                     /gene="folD"
                     /locus_tag="ACHJWA_002558"
     CDS             420..1286
                     /gene="folD"
                     /locus_tag="ACHJWA_002558"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFO4309136.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            1288..1500
                     /gene="ybcJ"
                     /locus_tag="ACHJWA_002559"
     CDS             1288..1500
                     /gene="ybcJ"
                     /locus_tag="ACHJWA_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFO4309137.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1608..2129
                     /gene="ybcI"
                     /locus_tag="ACHJWA_002560"
     CDS             1608..2129
                     /gene="ybcI"
                     /locus_tag="ACHJWA_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO4309138.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FNFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(2165..3550)
                     /gene="cysS"
                     /locus_tag="ACHJWA_002561"
     CDS             complement(2165..3550)
                     /gene="cysS"
                     /locus_tag="ACHJWA_002561"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFO4309139.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKVEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3724..4218
                     /gene="ppiB"
                     /locus_tag="ACHJWA_002562"
     CDS             3724..4218
                     /gene="ppiB"
                     /locus_tag="ACHJWA_002562"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFO4309140.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4221..4943
                     /gene="lpxH"
                     /locus_tag="ACHJWA_002563"
     CDS             4221..4943
                     /gene="lpxH"
                     /locus_tag="ACHJWA_002563"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFO4309141.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRTKVHKPWLQTLFLALPLFVRKRIAVRMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            5061..5570
                     /gene="purE"
                     /locus_tag="ACHJWA_002564"
     CDS             5061..5570
                     /gene="purE"
                     /locus_tag="ACHJWA_002564"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFO4309142.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            5567..6634
                     /gene="purK"
                     /locus_tag="ACHJWA_002565"
     CDS             5567..6634
                     /gene="purK"
                     /locus_tag="ACHJWA_002565"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFO4309143.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAE
                     RSEWPAVFERLGELAIVKRRTGGYDGRGQWRLRADETEQLPAECYGECIVEQGINFSG
                     EVSLVGARCFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLSATLEA
                     LIPLLPPEYASGVMWAQSKFC"
     gene            complement(6773..7666)
                     /gene="ybcF"
                     /locus_tag="ACHJWA_002566"
     CDS             complement(6773..7666)
                     /gene="ybcF"
                     /locus_tag="ACHJWA_002566"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO4309144.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVRTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTDDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGMPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7663..8478)
                     /gene="ylbF"
                     /locus_tag="ACHJWA_002567"
     CDS             complement(7663..8478)
                     /gene="ylbF"
                     /locus_tag="ACHJWA_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO4309145.1"
                     /translation="MTIIHPLLASSSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8489..9748)
                     /gene="ylbE"
                     /locus_tag="ACHJWA_002568"
     CDS             complement(8489..9748)
                     /gene="ylbE"
                     /locus_tag="ACHJWA_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO4309146.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGASGMEAAR
                     AVSEEMAEIYLERNMQLQIPSWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(9758..11386)
                     /gene="fdrA"
                     /locus_tag="ACHJWA_002569"
     CDS             complement(9758..11386)
                     /gene="fdrA"
                     /locus_tag="ACHJWA_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO4309147.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQAL
                     TQVRRWDSACQKLPDANLANQALDRNLNVMMFSDNVTLEDEIQLKTRAREKGLLVMGP
                     DCGTSMIAGTPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGEGITHAIGLGGRDL
                     SREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMKATGKPTVALFLGY
                     TPAVARDENVWFASSLDEAARLACLLSRVTARRNAIMPASSGFICGLYTGGTLAAEAA
                     GLLAGHLGVEADDAHHHGMMLDADGHQIIDLGDDFYTVGRPHPMIDPTLRNQLITDLG
                     AKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYAIATVTGTERDPQC
                     RSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSPAAQQHTPSLLENVAVINIGLRSF
                     ALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            11703..12752
                     /gene="allD"
                     /locus_tag="ACHJWA_002570"
     CDS             11703..12752
                     /gene="allD"
                     /locus_tag="ACHJWA_002570"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HFO4309148.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHVVINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRQAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            12774..14009
                     /gene="allC"
                     /locus_tag="ACHJWA_002571"
     CDS             12774..14009
                     /gene="allC"
                     /locus_tag="ACHJWA_002571"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFO4309149.1"
                     /translation="MITHFRQTIEEALPWLSSFGADPAGGMTRLLYSPEWLETQQQLK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVILSGSHIDTVVNGGNLDGQFGALAARL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPDAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIRIDIDLWMDEEPVPMNKDL
                     VATLTELCESEKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            14020..14805
                     /gene="allE"
                     /locus_tag="ACHJWA_002572"
     CDS             14020..14805
                     /gene="allE"
                     /locus_tag="ACHJWA_002572"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFO4309150.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFS
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGYAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(15033..16178)
                     /gene="glxK"
                     /locus_tag="ACHJWA_002573"
     CDS             complement(15033..16178)
                     /gene="glxK"
                     /locus_tag="ACHJWA_002573"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HFO4309151.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGEKVNAFYGITGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LVANGGNLARVASIEMEECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLRAV
                     NLEQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(16200..17325)
                     /locus_tag="ACHJWA_002574"
                     /pseudo
     CDS             complement(16200..17325)
                     /locus_tag="ACHJWA_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
     gene            complement(17382..18743)
                     /gene="allB"
                     /locus_tag="ACHJWA_002575"
                     /pseudo
     CDS             complement(17382..18743)
                     /gene="allB"
                     /locus_tag="ACHJWA_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
     gene            complement(18780..20171)
                     /gene="ybbW"
                     /locus_tag="ACHJWA_002576"
     CDS             complement(18780..20171)
                     /gene="ybbW"
                     /locus_tag="ACHJWA_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HFO4309152.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGLGPIFDYIPSG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPLMEPLS
                     RVSWFVGVIVAFA"
     gene            complement(20340..21218)
                     /gene="glxR"
                     /locus_tag="ACHJWA_002577"
     CDS             complement(20340..21218)
                     /gene="glxR"
                     /locus_tag="ACHJWA_002577"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO4309153.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     NVDTARQVTEAADIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(21319..22095)
                     /gene="hyi"
                     /locus_tag="ACHJWA_002578"
     CDS             complement(21319..22095)
                     /gene="hyi"
                     /locus_tag="ACHJWA_002578"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HFO4309154.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKNI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
     gene            complement(22108..23889)
                     /gene="gcl"
                     /locus_tag="ACHJWA_002579"
     CDS             complement(22108..23889)
                     /gene="gcl"
                     /locus_tag="ACHJWA_002579"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFO4309155.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(23979..24794)
                     /gene="allR"
                     /locus_tag="ACHJWA_002580"
     CDS             complement(23979..24794)
                     /gene="allR"
                     /locus_tag="ACHJWA_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFO4309156.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(24872..25354)
                     /gene="allA"
                     /locus_tag="ACHJWA_002581"
     CDS             complement(24872..25354)
                     /gene="allA"
                     /locus_tag="ACHJWA_002581"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFO4309157.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            25584..26510
                     /gene="allS"
                     /locus_tag="ACHJWA_002582"
     CDS             25584..26510
                     /gene="allS"
                     /locus_tag="ACHJWA_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFO4309158.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            26579..27673
                     /gene="mnmH"
                     /locus_tag="ACHJWA_002583"
     CDS             26579..27673
                     /gene="mnmH"
                     /locus_tag="ACHJWA_002583"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFO4309159.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLHAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQNLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLTTGSTDGHLAWLVPLLEEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAQ"
     gene            27789..28154
                     /gene="ylbG"
                     /locus_tag="ACHJWA_002584"
                     /pseudo
     CDS             27789..28154
                     /gene="ylbG"
                     /locus_tag="ACHJWA_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(28904..>29018)
                     /locus_tag="ACHJWA_002585"
     CDS             complement(28904..>29018)
                     /locus_tag="ACHJWA_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4309160.1"
                     /translation="PENDGVFTWLPDFFPHVAVDISIYTNVEDDYFFLIFP"
BASE COUNT         7176 a         7838 c         7170 g         6834 t
ORIGIN      
        1 aagacactga tagtagtaaa ttccttttta tcctctaaga atgtcttaat tgaaaatatg
       61 cactctattc taaaaaatag agagccccgt tagatgaata cttccgcgca aaatatattc
      121 aacacaaata tagacctgaa gcggtaaatt accaggctga aaattctttt tatattgtca
      181 ggtatttctt aaattatctt aatccttaaa caaggaaata aatcagttcc agatttacaa
      241 cgccatcatg gacgaaaaat gaagctttca atctcagcga cctcagcgac agtgcgccta
      301 accttcgcaa gaggtcgctt cacgcgataa atctgaaacg aaacctgaca gcgcgccccg
      361 cttctgacaa aataggcgca tccccttcga cctacgtaac agatggaatc ctctctctga
      421 tggcagcaaa gattattgac ggtaaaacga ttgcgcagca ggtgcgctct gaagttgctc
      481 aaaaagttca ggcgcgtatt gcagccggac tgcgggcacc aggactggcc gttgtgctgg
      541 tgggtagtaa ccctgcatcg caaatttatg tcgcaagcaa acgtaaggct tgtgaagaag
      601 tcgggttcgt ctcccgctct tatgacctcc cggaaaccac cagcgaagcg gagctgctgg
      661 agcttatcga tgcgctgaat gccgacaaca ccatcgatgg cattctggtt caactgccgt
      721 taccggcggg tattgataac gtcaaagtgc tggaacgtat acacccggac aaagacgtag
      781 acggtttcca tccttataac gtcggtcgtc tgtgccagcg cgcgccgcgt ctgcgtccct
      841 gcaccccgcg cggtattgtc acgctgcttg agcgttacaa cattgatacc ttcggcctca
      901 acgccgtggt gattggcgca tcgaatatcg ttggccgccc gatgagcatg gaactgttgc
      961 tcgcaggttg caccaccacc gtgactcacc gtttcactaa aaatctgcgt caccacgtag
     1021 aaaatgccga tctgttgatc gttgccgtcg gcaagccggg ctttattcct ggtgactgga
     1081 tcaaagaagg cgcaattgtg attgatgtcg gcatcaaccg tctggaaaat ggcaaagttg
     1141 tgggcgacgt cgtgtttgaa gacgcggcta aacgcgcctc atacattacg cctgttccgg
     1201 gcggcgttgg cccgatgacg gttgccacgc tgattgaaaa cacgctacag gcgtgcgttg
     1261 aatatcatga tccacagggt gagtaacatg gcgacatttt ctttaggtaa acatccgcac
     1321 gttgagctgt gcgacttgct gaaactggaa ggctggagcg aaagcggcgc gcaggcgaaa
     1381 atcgcgattg ccgaaggcca ggtgaaagtc gacggcgcgg ttgaaacgcg caaacgctgc
     1441 aaaatcgtcg ccgggcagac agtgagtttt gcaggtcaca gcgtacaggt tgttgcctga
     1501 tccctttccc ggcctgtatc aacaggccgg ttctcttttc agacttttgc cctctacgcc
     1561 ccgttcatcc atgctgatag catcgacatt ttgccgaagc gaccatcatg ccaaccgtta
     1621 ttacgcacgc cgccgttccc ctatgcattg gtttagggtt gggttcgaaa gtcatccctc
     1681 cgcgcctgtt atttgccgga atcatcctgg cgatgctgcc cgatgccgac gtcttgtcgt
     1741 ttaattttgg cgttgcttac ggcaatgttt ttggtcatcg cgggtttacc cattcgctgg
     1801 tgtttgcgtt tgttgtcccg ctgttatgtg tgctgattgg acgtcgatgg ttcagggctg
     1861 ggctgattcg ctgctggctg tttttaaccg tctcgttgct atcgcacagc ttgctggatt
     1921 cggtaaccac tggcggtaaa ggggttggct ggctgtggcc gtggtcagat gaacgctttt
     1981 tcgctccctg gcaggtgatt aaagtcgcgc cgtttgcatt gtctcgttac accacagcgt
     2041 acgggcatca ggtgattatt tctgaattga tgtgggtgtg gttgccgggg atgctactga
     2101 tgggaatgtt gtggtggcgc agacgataac cggatgcgaa aactcgcatc cggcaatagc
     2161 gcaattactt acgacgccag gtggtccctt gcgggccatc ttccagcacg atccccatct
     2221 cattaagacg gtcacgcgcc gcatctgccg ccgcccagtc tttcgcttta cgggcatcca
     2281 gacgctgttg aattaacgct tcaatctcag ccacttcgct gtcgtctgcc tgcgcgccgc
     2341 tttgcaggaa cgcttccggt tcttgctcca gcaggcccaa tacggcggaa agtttacgca
     2401 ggtgagatgc cattgcattc gccgctgcca tatcttctac tttcagacgg tttacttcac
     2461 gcgccatatc aaacagcacg gaataggctt ccggggtgtt gaaatcgtcg tccatcgctt
     2521 caataaagcg cgcttcaaac gcttcgccac cggcaggcgc aacggtttta tctgtgccgc
     2581 gcagcgcagt gtagagacgc tccagcgccg cacgcgcctg cttcaggttc tcttcgctat
     2641 agttcagctg gctgcgatag tggcccgaca tcaggaagta acgcacggtt tccgcgtcgt
     2701 agtatttcag cacatcgcgc acggtaaaga agttacccag cgatttggac atcttctcgc
     2761 ggtcaaccat caccatcccc gagtgcatcc agtagttcac atactgacca tcatgggcac
     2821 aggtggactg cgcgatttcg ttttcgtggt gcgggaacat caggtctgaa ccgccgccgt
     2881 ggatatcaaa gtggttaccc agctgcttgc agttcattgc cgaacattca atgtgccagc
     2941 caggacgacc cgcgccccac ggagacggcc agctcggttc gccctctttc gacatcttcc
     3001 acagaacgaa gtccattggg ttgcgtttgt cgtcgaccac gtcaacgcgc gcgcctgcct
     3061 gcagctggtc gagatcctga cgcgacagca cgccataagt tggatcggtc gggacgtcga
     3121 acatcacgtc gccgttgtcc gccacataag cgtgaccttt ggcgatcagt tgttcagtga
     3181 gttcaataat ttctgcgata tggtgcgtcg cgcgcggctc catatccggg cgcagaatgt
     3241 tcaacgcatc aaaatctttg tgcatttcgg cgatcatgcg atccaccagc gccacaaagc
     3301 tttcgccatt ttcattggcg cgtttgatga ttttgtcgtc gatatcggta atgttgcgca
     3361 catacttcag cttatagccg aggaaacgca gatagcgcgc aaccacgtca aaagcaacaa
     3421 aggtacgccc gtgaccgata tgacagagat cgtaaacggt gattccacac acgtacatgc
     3481 cgacttcccc ggcgtgaata ggcttaaatt cctctttttg gcgtgtcaga gtattgaaga
     3541 tttttagcat cgaagattcc gtttagacat gtgtgggtaa ttgagttgcg tataataccc
     3601 atatttcccg cgcgaatcag catacattgc gtgatgatcc gatcgtgcgg ttatgctata
     3661 acaccaccct atatatgacc cgaactgggt tgaagcacca atcaaacgga acaggatgca
     3721 aaaatggtta ctttccacac caatcacggc gatattgtca tcaaaacttt tgacgataaa
     3781 gcacctgaaa cagttaaaaa cttcctggac tactgccgcg aaggttttta caacaacacc
     3841 attttccacc gtgttatcaa cggctttatg attcagggcg gcggttttga accgggcatg
     3901 aaacaaaaag ccaccaaaga accgatcaaa aacgaagcca acaacggcct gaaaaatacc
     3961 cgtggtacgc tggcaatggc ccgtactcag gctccgcact ctgcaactgc acagttcttc
     4021 atcaacgtgg ttgataacga cttcctgaac ttctctggcg aaagcctgca aggttggggc
     4081 tactgcgtgt ttgctgaagt ggttgaaggc atggacgtgg tagacaaaat caaaggtgtt
     4141 gcaaccggtc gtagcggtat gcaccaggac gtgccaaaag aagacgttat cattgaaagc
     4201 gtgaccgtta gcgagtaatc gtggcgacac tctttattgc agatcttcat ctctgcgtgg
     4261 aagaaccggc gatcaccgcc ggttttctgc gttttttagc gggagaagcc cgcaaggccg
     4321 acgcgctgta tattcttggc gatctgtttg aagcatggat tggcgacgac gatcccaacc
     4381 cactccatcg ccagatggcg gcagcgatca aagcggtgtc cgattccggc gttccctgtt
     4441 atttcattca tggcaaccgt gattttctgc tcggcaaacg ctttgcccgt gaaagtggca
     4501 tgacgttatt gccggaagaa aaagtgctcg aactttatgg tcgtcgggtg ctgattatgc
     4561 atggcgacac gctgtgcacc gatgacgcgg gttatcaggc ttttcgcacc aaagtccaca
     4621 aaccctggct gcaaacgcta ttcctcgctc tgccgttgtt tgtgcgcaaa cgcattgccg
     4681 tgcgaatgcg cgcgaacagt aaagaagcca acagcagtaa atcgctggcg atcatggacg
     4741 ttaaccaaaa cgcggtagtc agtgcgatgg aaaaacatca ggtgcaatgg ctgatccacg
     4801 ggcataccca tcgcccggcg gtgcatgaac ttatcgccaa tcagcaacct gcttttcgcg
     4861 tggtactggg tgcctggcat acggaaggtt cgatggtgaa agtcacggcg gatgacgttg
     4921 agctgattca ttttccgttt taaaaaaccc gcaactttgc tgatttcaca gccacgcaac
     4981 cgttttcctt gctctctttc cgtgctattc tctgtgccct ctaaagccga gagttgtgca
     5041 ccacaggagt tttaagacgc atgtcttccc gcaataatcc ggcgcgtgtc gccatcgtga
     5101 tggggtccaa aagcgactgg gctaccatgc agttcgccgc cgaaatcttc gaaatcctga
     5161 atgtcccgca ccacgttgaa gtggtttctg ctcaccgcac ccccgataaa ctattcagct
     5221 tcgccgaaag tgccgaagag aacggttatc aggtgattat tgcgggcgca ggcggcgcag
     5281 cgcacctgcc aggcatgatt gccgccaaaa cgctggtgcc agtgctgggc gtgccagtac
     5341 agagcgccgc actgagcggt gtcgatagcc tctactccat cgtacaaatg ccacgcggca
     5401 ttccggtggg tacgctggcg attggtaaag ctggcgcggc aaacgcggca ttactggcag
     5461 cacaaattct tgcgactcat gataaagaac tgcaccagcg tctgaatgac tggcgcaaag
     5521 cacaaactga tgaagtgctg gaaaacccgg acccgcgagg tgcggcatga aacaggtttg
     5581 cgtcctcggt aacgggcagt taggccgtat gctgcgtcag gcaggcgaac cgttaggcat
     5641 tgctgtctgg ccggtcgggc tggacgctga accggcggcg gtgccttttc aacaaagcgt
     5701 gattaccgct gagatcgaac gctggccgga aacagcatta acccgcgagc tggcgcgcca
     5761 tccggccttt gtgaaccgcg atgtgttccc gattattgct gaccgtctga ctcagaagca
     5821 gcttttcgat aagctccacc tgccgactgc accgtggcag ttacttgccg aacgcagcga
     5881 gtggcctgcg gtgtttgagc gtttaggtga gctggcgatt gttaagcgtc gcactggtgg
     5941 ctatgacgga cgcggtcaat ggcgtttacg cgccgatgaa accgagcagt taccggctga
     6001 atgctacggc gaatgtattg tcgagcaggg cataaacttc tctggtgaag tgtcgctggt
     6061 tggcgcgcgc tgctttgatg gcagcaccgt gttttatccg ctgacgcata acctgcatca
     6121 ggacggtatt ttgcgcacca gcgtcgcttt tccgcaggcc aatgcgcagc agcaagcgca
     6181 agccgaagag atgctgtcgg cgattatgca ggagctgggc tatgtgggcg tgatggcgat
     6241 ggagtgtttt gtcaccccgc aaggtctgct gatcaacgaa ctggcaccgc gtgtgcataa
     6301 cagcggtcac tggacacaaa acggtgccag catcagccag tttgagctac atctgcgggc
     6361 gattaccgat ctgccgttac cgcaaccagt ggtgaataat ccgtcggtga tgatcaatct
     6421 gattggtagc gatgtgaatt atgactggct gaaactgccg ctggtgcatc tgcactggta
     6481 cgacaaagaa gtccgtccgg gccgtaaagt ggggcatctg aatttgaccg acagcgacac
     6541 atcgcgtctg tccgcgacgc tggaagcctt gatcccgctg ctgccgccgg aatatgccag
     6601 cggcgtgatg tgggcgcaga gtaagttctg ttaacctggt gctcatttga ttgccggatg
     6661 cggcgtgaac gccttatccg gcctacaccg atccggtacc cattgtaggc ctgataagac
     6721 gcgtcaagcg tcgcatcagg catggtgcgc cgattgccgg atgctctcat cactacagcg
     6781 aaatacaggt ccccgcttcg cccgccagcg tctcttcaat tcgcgataac gccccaatcc
     6841 acgcgggttt accacggctt ctgacataac cactcaccgc cgttaccttc ggccccatcg
     6901 aaccatcggc tttggcaaat ggcgctaact catccggtgt ggcatggcga atggcacgtt
     6961 gctgcggcat tccccagttt tcatataccg catcagcatc ggtgaggatc accagtccat
     7021 cggcgttaat ttgctcggcg agcaacgcag cggcgagatc tttatcaatc actgcttcac
     7081 tccctgctcc gtcatccgtc acaggcacac cgccgccgcc actgcaaatc accacatgcc
     7141 cctctttgag caacaactcg atggcttcgc tgtcgagaat tttacgcggt tgcggagacg
     7201 ccaccacccg gcgcaaatat ttaccatcac gtttcatctg ccagccgtaa gccgcttcca
     7261 gtgcctcttg ttcttctggc tgataaaccg gaccaataaa tttctctggc tgcaaaaacg
     7321 ccggatcatc aggcgaaact tcaatgcgcg tccgcaccgt cgtgacaggc ggcatctgcg
     7381 gctgtgcgct caaactctgc gccagcatat agccaatcat cccctgactt tctgccacca
     7441 gcacatccag cggatacggt tctacctctt tccacgccag attctgcaat gccagcagcc
     7501 ccacctgcgg cccgttgccg tgaacaatcg ccagccgata agaacgggcc aggcgcgcca
     7561 gcgcgggtac agcactggcg atattgcgat attgattttc tgccgtcagc gcctcaccgc
     7621 gctggagtaa ggcgttgccc ccaagagcta caaccagtgt tttcatggtt ttccttgtaa
     7681 taattgttgg ccaagccaga accccagcag tgtatctgcc cctgacgtat gccccagcgc
     7741 cagcagcgaa tcaatcgcaa cagcggtacg tttcgggcaa ctcagagcat gaacaaagtg
     7801 caggagtggc gaggcgaaat atccttgcgc ggcataacgt aaataactga cgctcaccgc
     7861 tgtggtaacg agttgcagat tgtcggaaca ggcaaaaaac ggacgaccgg agcgcgcatc
     7921 taaagcacca taataccagg ccgccagcag cattccgctc agcgtgtcat catgacttgg
     7981 cgttaatccg gggcctttac ccagccagtg ccgccagtcg gtcttaacgc cattgagcgc
     8041 ggcctgaaaa cagtgacgaa actggcgtaa ctcagcaggc agcggatcgc ttgccgccaa
     8101 cgccagtggc ccgaaaagcc cggtttcctc cgcgcgttgc atccatgcag ctgcaagtgg
     8161 ttgaggatgc gcaggcggcg taatacgcag caaacaataa cgctgtggct gtttaaccgt
     8221 gaaacgcccg aggcgaatcc cttgagccac aacctgtggt cgttcattgc cgcataatcc
     8281 accgcataac gcatcgaatt gcgcacggcg aagcacccat cctccggggc cgaaaccact
     8341 accctgacga tgcagcgtta acagttcgcc gctttccgtc atcaggttaa tcgcccgccg
     8401 ccacactccc gctaaccgcc aggactggcg ataattcggt gcgctactac tggcaagcag
     8461 aggatggatg atcgtcatta cgcgttcctc aaccaatccc catgctttca gccagtgctt
     8521 ccagcgcctg ttcaaagcac gccagcggtg cccgcacggt gcctgcgcca atctgcccga
     8581 tccccgcctc tttatgggcg ataccggtat tgatgagtgg cgtaatgccg gtttctacca
     8641 cgcgacgaat gtccagtccc aggcacgcgc cctgaaaatc ccagcttggg atctgcaact
     8701 gcatattgcg ttcaaggtaa atttccgcca tctcttcaga taccgccctg gccgcttcca
     8761 tgccactcgc accgacaaag cgcgttacgc caggcgctgc gatcattgcc gcacctccga
     8821 taccaaaggt ttcggtaatc gcgctatcgc ccatatccgg gttcgcctgc tcctgcgaga
     8881 agccggtgaa aaacagacct tgcggagtgt ttacaggcgc agtaaaccag cgttcgccca
     8941 gcccgcttac ccgaatcccg aacatattgc cgttgcgggt cattgccgtg acgatgctgc
     9001 ctgcgcggat catcgcgcca gcatccatcg ccgccttgca gtaagccatc gcgaggttga
     9061 ggaagaactg atcggtcacg ctgaggaaat ccatcacttc cgcgatgtgc tgtttatcat
     9121 gatcgaggcg agcaatttgt ggggccagcg cacgcattaa cagtgctgag gaagcaatat
     9181 tgcgttgatg gaactcatcg cccatcgtaa tgccctgcgc catcatcgca gtaagatcga
     9241 taccgcgctc catgcgcccc agcgccgcgc ttaatactgg cattaacaca tcgcgcatcc
     9301 agcgatggcg agtcaggaca tcttcgccgt aagcgccaaa acgcatcact ttgccgatac
     9361 cttcgttgag gttgcagtac gcccggttgc cgtcggtcac gttctcgacc accagcatcg
     9421 gcatactggc agaagtaata ccgcccattg gcccgacggc attcacatgg tgacaaggaa
     9481 tgaagttcac ttccccctgc tccagtattg ccagcgcctg cgcttcatct ttcgcccaac
     9541 cttcgaacag acatgcgccc acgcacgccc ctttcatggg tccggtcatc tcctgccagc
     9601 gcattggcgg cccggcgtga agcagtgttt tgccctcgtt tagttcgctg ataagtgaag
     9661 aagccggttg cacatccagc cagtgtggac gagcgcgacg aatttgttcg ataaccgcag
     9721 cattggcttg cgccactgat gtaaacatga gaacccctta ttgcaaacgt tctaataaac
     9781 gagccagttt tttattgccg ccggcgactg gcgaccattg gtaatgcaca accggtttgc
     9841 tggcgctttg tagctccagc gcaaaactgc gtaatccgat gttaatcacg gcgacgtttt
     9901 ccagtaatga cggtgtgtgt tgctgtgcgg caggcgagag cggatgaatt aacgctgccg
     9961 ccagcaaggt ggcttccggt agcgaactca cgaccgcaat ccccgcatct tccagcgtgg
    10021 cgatttgctg cgagcggcat tgcgggtcac gttcagtgcc tgtcaccgtg gcaatggcat
    10081 acagtggttg attgtctgaa cgcgcagcac aggctttttg ccaggcgctt accagcgagg
    10141 cggcaggatc ggcggtcgca ccgaagccaa tcacgacatc aagcagcagc acacgcactt
    10201 gcggtttagc gccgagatcg gtaattaact ggttgcgtaa ggttgggtcg atcatcggat
    10261 ggggacgccc gacggtgtag aaatcatcgc cgaggtcaat aatctggtgg ccatcggcgt
    10321 ccagcatcat gccgtgatga tgggcatcat cggcttccac gccaaggtgt ccggcaagta
    10381 atcccgccgc ttcggcagcc agcgtaccgc cggtatacaa accgcaaata aatccgctgc
    10441 tggcaggcat tatcgcgtta cgtcgcgccg taacgcgtga aagcaggcaa gccaggcgtg
    10501 cggcctcatc cagcgaggag gcaaaccaga cattctcgtc gcgggccacc gccggggtat
    10561 aacctaaaaa cagcgccacc gtcggtttgc cggttgcttt catggcatta acaattttca
    10621 gacgcaccgc ttcggcaggt ggttttgaaa caaatgccag cacttcgctt ttctcgtctg
    10681 cactgagcat ttccagcgcc gtcagcgcac tgatgccgcc cacttcacgg ctgaggtcgc
    10741 ggccgccaag gccaatcgcg tgagtaattc cctcccctgc cagcgcaatc tgcgagcaca
    10801 gctcctgaat cccggtaccg gaagcgccaa tgacgccaat gttgccttcc ggcatcacgt
    10861 tagcaaaagc cagcggcgtg ccggcaatca tcgacgtacc gcagtccggc cccatcacca
    10921 gcaagccttt ttcccgcgcg cgggttttaa gttggatttc atcttccagc gtgacgttat
    10981 cggagaacat catcacgttg aggttgcgat ccagcgcctg attcgccaga ttggcatcgg
    11041 gtaatttctg acaggcactg tcccagcgac gcacctgcgt caacgcctgg ctgctgcctg
    11101 acccctgcgc cagttgtttt agcgcctctt caagctgctg cataatcgcc tgcgcgatcc
    11161 ccgcatccgc cgcttcgcta cgaattgcca cgcaaatatc gttcggcgtg gcgttattaa
    11221 aatcgtcatg ccagaaacct gtggtatcta ataacgcttt attggcgggc gtacccatca
    11281 ttacggaaac atcatcaaca ttttctgatt cgctgagttt tcgtgaaata atcattaaac
    11341 tgaccgaatc ctgaaaacac ccttttttaa taaaggcgtg gatcataaca actccttaaa
    11401 aattgcaccc atggcaattg cacgagcagt gttaatgtgg cgattacggt atcgatgaca
    11461 acgagtaaat aaggtgatga atatcacaca gggattatta acaaaagaat tcaaataacc
    11521 gccatgctat tacgtgttat tttttaataa gcgtggttgt tttaaaaacc aggttaaaag
    11581 cgagcggatt atcaaataaa ttctaactat atttttttgc ctgtctggat cacataatcc
    11641 agatattttc cctgtatgtt aattgcagtc atgcttcaca ccgtcgttaa aaaggaagac
    11701 agatgaaaat cagtcgggaa acactccacc agctaattga gaataaactc tgccaggctg
    11761 ggttaaaacg tgagcacgct gcaaccgtgg ctgaagtatt ggtttatgcc gatgccagag
    11821 ggatccactc tcatggcgcg gtgcgcgtgg aatactacgc ggaacgcatt tcaaaaggcg
    11881 gcaccaaccg cgaaccggag tttcgtcttg aggaaaccgg gccgtgctcg gcaattttac
    11941 atgccgacaa tgccgccgga caggtcgcgg cgaaaatggg tatggaacat gccatcaaaa
    12001 ccgcccagca aaatggcgtt gcggtggtcg gtatcagccg gatgggtcac agcggcgcaa
    12061 tctcttattt tgtgcagcag gcagcccgcg ccggattaat tggcatttcg atgtgccagt
    12121 ccgatccgat ggtggtgccg tttggcggcg cggaaattta ctacggtact aacccactgg
    12181 cctttgccgc gccgggagaa ggcgacgaga tccttacctt tgatatggcg actaccgtac
    12241 aggcatgggg aaaagtcctc gacgcccgct cgcgtaatat gtctatcccg gatacctggg
    12301 cggtcgataa aaacggtgca ccaacaaccg atccgttcgc ggtacatgct ctgctccccg
    12361 cagccgggcc gaaagggtat ggcctgatga tgatgattga cgtcctctca ggcgtcttac
    12421 tcggcttacc gttcgggcga caggttagtt cgatgtatga cgatttacac gccgggcgta
    12481 atttggggca attacatgta gttattaacc cgaacttttt ctcctccagc gaattattcc
    12541 gtcaacatct tagccagacc atgcgcgaat taaatgccat tacccccgcg cccggtttta
    12601 atcaggttta ttatcccgga caggatcagg atattaaaca acgccaagcc gccgtcgaag
    12661 gcatcgaaat tgttgatgat atttaccagt atttaatttc cgacgcgctt tataacacgt
    12721 catacgaaac gaaaaatccc tttgcgcaat aattattgag acaggacttt cttatgatta
    12781 cacattttcg tcaaaccata gaagaagcgc tgccctggct ttcctctttt ggcgctgacc
    12841 cagcgggtgg gatgacccgt ttactttatt cgccggaatg gctggaaacc cagcagcaat
    12901 taaaaaaaag aatggccgca agtgggctgg aaacacgttt cgatgaagtg gggaatttat
    12961 acggtcgcct gagtggcacc gaatatccac aggaagtgat tctgagcggt tcgcatatcg
    13021 ataccgtggt taacggcggt aacctcgacg ggcaattcgg cgcgctggcg gcgcggctgg
    13081 caattgactg gctgaaaact caatacggcg caccgctgcg tacggttgaa gtggtggcga
    13141 tggcggaaga agaaggtagc cgcttcccgt atgttttctg gggcagtaaa aatatctttg
    13201 ggctggcgaa tcctgacgac gtgcggaata tctgtgatgc caaaggaaat agttttgtcg
    13261 atgcgatgaa ggcttgcgga tttactctgc cggacgcccc gctaactccg cgtcaggata
    13321 ttaaagcctt tgtcgaactg catatcgaac agggctgtgt gctggaaagt aatgggcaat
    13381 caattggcgt ggtgaatgca attgtcgggc aacgtcgcta tacggtgacg ctgaacggcg
    13441 aatcaaacca tgcaggcacc acgccgatgg gttatcgtcg tgatacggtt tacgctttca
    13501 gtcgcatttg ccatcagtcg atcgaaaaag cgaaaaagat gggcgatccg ctggttctga
    13561 cctttgggaa agtagagccg cgcccgaata cggtgaatgt ggtgccgggt aaaaccacgt
    13621 tcaccattga ttgtcgtcat accgacgccg ccgtgctgcg tgatttcacc caacagttag
    13681 aaaacgacat gcgggcgatt tgcgatgaaa tggacattcg tattgatatc gatttatgga
    13741 tggacgaaga acccgtgccg atgaataagg acctggtcgc caccctgaca gaattgtgtg
    13801 aaagtgaaaa actgaattac cgggtgatgc acagtggtgc cgggcacgac gcgcaaattt
    13861 tcgcgcctcg cgtaccaacc tgcatgattt ttatccccag catcaacggg atcagccata
    13921 acccggcgga acgcaccaat attaccgacc ttgccgaagg ggtcaaaacg ttggcactca
    13981 tgctttatca acttgcctgg cagaaataag gagtcataaa tgggatattt aaataacgtc
    14041 accggttacc gcgatgattt actggctaac cgtgcgattg tgaaacacgg taatttcgca
    14101 ctgttaacac cagacggtct ggtaaaaaat attattccgg gctttgaaaa ttgtgacgcg
    14161 acaatcctct ccacgccaaa gctgggtgcc tcttttgttg attatctggt cacactgcat
    14221 caaaacggcg gcaaccaaca gggcttcggt ggcgaaggca ttgaaacgtt cctgtatgtg
    14281 atctctggaa atatcactgc caaagccgaa ggcaaaacat tttccttaag cgaaggtggc
    14341 tatctttatt gcccgcctgg ctccttaatg acgtttgtta acgcccaggc cgaagacagc
    14401 caaatctttt tatataagcg ccgctatgtt ccggtagaag gctatgcacc gtggctggtt
    14461 tctggcaatg ccagcgaact ggaacgcatt cactatgaag gtatggatga tgtgatcctg
    14521 ctggatttcc tgcccaaaga gttaggtttt gatatgaaca tgcatatcct ttcttttgca
    14581 ccaggtgcca gccacggtta tatcgaaaca catgttcagg aacacggtgc ctatattctt
    14641 tccggtcagg gggtttataa cctcgacaat aactggatcc cggtgaaaaa aggcgattac
    14701 atctttatgg gcgcttattc tttacaggct ggttatggcg tagggcgtgg tgaagcgttc
    14761 agctatattt actcgaaaga ttgtaaccgc gacgtagaga tttaagtcat accactcgcc
    14821 tgattatttt tagctatgtt ggattttgcc acggtttata ccgtggcttt ttttgcaaca
    14881 tagcctgatg cgcttcgctt atcaggccta catgatctct gcaatatata gaatttgcgc
    14941 gattttcagg aaggatatgg tactcatgcc gcatccggca tgagtactgc actcttatgc
    15001 gatataaatc gcatccgctt taaaggtaag ggtcagtttt taattccctg acctatttta
    15061 atggcgcagg caatatttcg cgcgctgttg aagagattgg tttcaccgct ggcgagcact
    15121 tcggctaaag gtgccagacg tggcaaaatg ctgaataccg catcaatgcc gtactggtgc
    15181 accacttcca cgccatcacc caatacgcca gcaatcccaa tcaccggtac attaaactgc
    15241 ttcgccaccg acgcaacgcc cagcggcgct ttaccgcctg ccgtttgcga gtcaatgcgc
    15301 ccttccccgg tgatcaccag tgctgccccc tgcaccgcct gctccaggtt gaccgcccgc
    15361 agtacaatct caatacccgg tttaatatcc gcattgagga ataccgccgc cgcaatcccc
    15421 ataccgcccg cagcgccacc gcccgccatc tggcagacat taatttcagt ttgctgttgt
    15481 aaaacacggg cgtaattttg cagcccctgt tcaagttctt cgaccatctc cggcgttgcc
    15541 cctttttgcg ggccaaacac cgccgccgcg ccgcgtgccc ctaccagcgg gttatcaacg
    15601 tcacatgcta cttcaatatg gcagttcgcc agacgcggat cgcattcctc catctcaatg
    15661 cttgccacgc gcgctaaatt gccgccgtta gccaccagca cctgaccgtc ggcatcaagg
    15721 aaacgcacac cgagcgcctg cgccatgccc ataccgccgt cgaccgtcgc actgccgcca
    15781 atgccgagaa taatatggcg aatgccgtta tccagcgcat gacgaattaa ctcccccgta
    15841 ccaaaactgg aggccagcaa cggattacgc ttttcaggcg cgaccagcat caggccactt
    15901 gctgccgcca tctcaatcac cgccgttttc ccgtcgccgg taatgccata aaaagcattc
    15961 actttttcgc ccatcggccc gcagacttca agcgtcacga tgttgccgcc cgtcgcggcg
    16021 accatcgcat ccaccgtccc ttcgccgcca tccgctatcg gcaaacagat atagttcgca
    16081 tcgggaaaga gggtcgaaaa cccggcttta attgcctgac aacatttttc tgcactcaag
    16141 ctctctttaa aagagtctgg cgcaatgaca atcttcatta tcaatccttt ttcaacaaat
    16201 tattcgatgc gttcccacgg aataaggtta tccataagaa cagccagtaa aatcccgacc
    16261 aataagccgt tactgagcag aggacgaagc gttaatggaa ggtcttgcag atacacaggc
    16321 ggtaatgcca ttaaaaatat gccgacaaat aacgctgcca taaataaacg aacgccgcgt
    16381 gtaatcgcca gtctgggtta ataaaccaat ggatgaaaca aacggtgaaa atggaattac
    16441 tgccagcggc acggttatca gtgtcataaa tccggtcgcc acaaagctac gacgataacg
    16501 cgtattcccc gcgccctgct gcggataaaa aacatccgtg ccccgaatcg caccgtaggt
    16561 attgctgata ttcaccagac ctgtaatcac cgccgtcaga ataatccccg gcgacaaagc
    16621 accgccactg ccgagcggga accactgcca gtgcaactca ccggagagcg aatgcgaaga
    16681 aggaaagcaa aagtaccaca acaaccagcc ggttatggtg ccgaccagca ggccataacg
    16741 ggcaaaacgt tgcggcagaa agataatcat cgccagtacc aggcacatca ccgccaccga
    16801 gagcgcgaac ggcggtaact gaattttaaa attcgggtcg gctatgccaa acggcagccc
    16861 gagcatacct ttgaaaaaga tagtggtcag ctgcgcgccc agcatcaaca taaacaagac
    16921 catcaccgac ggcgtaaata accgcgccag gcgatgacct aatccgctaa aaccaatcaa
    16981 catcgtcagc acgccggaga gtgcaatccc caccgccagg ctggtagcga tatcgttgat
    17041 cggtgtcccg cgcgatgctt cgccaagggt gatagttagg atggttcccc accacaggcc
    17101 ccccggccct tccataatag cgcgacgatg accgcaaaac gcctgagcga agcaggccag
    17161 tgcggtagca agaaaagcat attgcgtgag cgtaagcagg ctactttgcg gcaactgaaa
    17221 agcagaaagt agcgtaggag gaaccacaac cgtgttgcaa aaaataaaga aaaaccactg
    17281 aaatcctgat aacaggcttt cgcggctaac tgcaaaattg aacataaaca caccttaacc
    17341 ggagaatgcc cgccgcaagg acgggcattg caggggccag attactgctg atgtttaagg
    17401 ataaattgac ctttcggcgc aacagggaag ccctgttcga tgtcgtaaat cacatcacca
    17461 cgtaagatgg ttttcgtgat acgcgcgcca atggtacggc caacatacgg gctgactttg
    17521 tggcgatatt ccagatcgtc attggtaaga acatagctgc tattcggctg aatgaagacg
    17581 aagtcggcat cttttcctgg ggcgatacgg cctttttgct gcagaccaaa aatatctgct
    17641 gcgttagtcg ccattaattt gccgaacatt ggcagagaca ttccgcgttt ctgtaccgct
    17701 tcatcgaaca tcacgtccat acagttttgc agaccggcaa taccgcccca tgcttccatg
    17761 atgttgccgg ctttcatttc cggagggcat ggtgagtggt cggaaaccag gcggtcgatt
    17821 tcaccgttaa acagtttttc ccacatgcct ttctagtttt ccagatcgcg gatcggcggt
    17881 gaacacttcg ccagagtacc gatttcttcg aactgatcgg tatccagtac gaagtaatgc
    17941 gggcaggatt cacaagtaac gtcctgacct tcctgacgtg cacgagtcac ttcttcaacg
    18001 ccttccgggc tgctgatatg gcaaacgtgc agacggcaac cggcaacttt cgccagatac
    18061 agcacgcggc gaatcgcttc cacttcggta aataccggac gcgaagccac atagtcatgg
    18121 gcagttacgc gaccttcacg tttcgcttct tcgcccagtg cgtcacagat cagcgcgttt
    18181 tcgcagtgca ccagcaccgg ctgccccagt tcgcccagct tctgcgcacc tttgaagaac
    18241 tgccagtcgt ttacatcacg gaagtcgttg tcgataccgc gatcgccaca ggtcgcaacg
    18301 aagcatttga agccgacaac gcccacttca tccagctcat gcagacggtc gatgttgtaa
    18361 gacaccaggc caccgagttg tgccgcatcg atagtcagct tgcctttagc ggcatcgaac
    18421 ttcagttcaa ttgaagcgcg gtcaaccgtt gcaggcagct ggttgagcgg catttcgatc
    18481 atggtggtga taccaccttt tgccgctgcg cgagtaccgg tttcataacc ttcccagtgg
    18541 ctacgacccg gttcagaaat atgggtgtgc gcatcaacca tgcccggcga aaccaccaga
    18601 ccagacgcat ccataacttc ttttgcatcg cccagatcct gaccgatagc agcaattttg
    18661 ccgcctttaa cggcgatatc tacaacgcga gcttcgtttt ctaaaataac ggtgccgttt
    18721 ttaatgatta aatcaaaaga cataacaaac tccttgtaaa atacagattg gtttttaact
    18781 caagcaaagg cgacgatgac gccgacaaac catgaaacac gcgataacgg ttccattaag
    18841 ggaataaact taccgccaag agataaaata acggccacca gagttactga aaacgcggtg
    18901 aggttaaaac cgttatcgta atatttataa tcgccaggtg cggtgtacag ttcatcaaga
    18961 ttaatttgcc cacgcatcac cacaaaataa tgcgccatca tgacaccaat taccggacca
    19021 agcattccgc cgataatatc gaggaacaga taaatactgt cctgattttc cattaatttc
    19081 cacgggcaga tcagcaggct gataatactg gcaatcagta cgccgttttt ataggtcagt
    19141 tttgtcggtg caatggcagc aatctgataa ccagccggaa taatattacc agtggcgtta
    19201 gtggagatag tcgtcatcag aataaccagt accgcaaaga acgaggcaaa caggctgtcc
    19261 caacgctgaa caatatccag cacgttccag gtatcagcgc cgtaatgaat actggctccg
    19321 gcaataatac atacccccgc gaccgcaaac agaatatagg ccacaactaa acccagcgtt
    19381 tgccccagcg cttgctcacg aaacgagtgg gcgttttgcg taaagtcgga tgcgctcacc
    19441 gccggtgccg cccagaccgc aactaccgcg ttaatcacca ccaggaacaa gaatccactg
    19501 ttttctgctt tctgaatacc gctcggaatg tagtcaaaga ttggaccgag cccgaccagc
    19561 gaaatcgccc aaatcgccat accgccgaaa acgatataga tgcacgggtt aagaatggca
    19621 gtgaatttat ttaaaacttt gccaccgcca aaacctatac caacgttgac cagccagaag
    19681 agtaagaaag taattaagcc cggtagagaa aggcctaaca gagtgaaatc accaccgaga
    19741 gtcaaaaatc ccggccagat tttgccaatc agaatcaagc aggccagtga ccccgcgtaa
    19801 cattgcaggc caaaccacat aatggcagca ataccgcctc ttaataatcc gggaaacagt
    19861 gcgccacgta cgccgtaaga agcacgcagg atcatggcaa acggtacgcc gtatttactg
    19921 cccgcagcgc cgtttaatac cattaccgcg gcaatgaaaa aggcgctgag gataattgcc
    19981 agcataatac taaaggtaga caagccgaga ataaaaaagc cgccgaccat cacataattg
    20041 ggaacgttat gaaccgaacc catccataag gtaaaatagt taaatgtttt ccaggtccgc
    20101 tggctttgcg ttttcggcaa tagatcttcg ctatagccgc gttgctggaa tagttttctc
    20161 tgatgttcca taaatccctc ttaatttcac gtatcgaata acgttgtttt aatctagcta
    20221 tgaggaaaaa ataaaataaa aatattattt ttaatactga agcaacatct ttggctgctt
    20281 tcttttttaa tattgctggc cgattcattt tttgatatcg gccattttta ttgcgggtat
    20341 caggccagtt tatggttagc cattaattcc agcgcctgca ctaacgcaga gtgatccaac
    20401 tggctgccac cgtttgccgc gcaggtatta aataactcct ggcaggtcgc agtgtttggc
    20461 aggttcagag ccagtgcctt cgcactttgc agtgccaggt tgagatcttt ctggtgcagc
    20521 gcaattttga aacctggatt aaaggtgcgt ttaatcatgc gctcaccgtg aacttccaga
    20581 atacgtgagg aagcaaagcc gcccatcagc gcctggcgca cacgtaccgg gtctgcaccg
    20641 gcttttgaag caaacagcag agcttcagag accgcttcaa tattgagcgc tacgataatc
    20701 tgatttgcca ctttgcaggt ttgaccgtcg ccgttaccgc ccacaagggt gatattttta
    20761 ccgagcagtt caaacagcgg tttaacacgt tcaaataccg cttcgtcacc gccaaccata
    20821 attgacaacg tcccttcacg cgcgccgatt tcgccgccgg agactggcgc atcgagataa
    20881 tcaccaccca gttcattcac ctgacgagcg aaacgtttag tttcaatcgg tgaaatggag
    20941 ctcatatcaa caatggtttt gcccttcagc gaggctttgg tacaaccatt ttcaccaaac
    21001 agaacttctt caacctgagg tgtgtccggc accataataa aaatgatgtc cgctgcttcc
    21061 gttacctggc gcgcggtgtc gacattaaca gcaccgagtg acagtaattc atcagcaacc
    21121 ggtccaatgg tcgtgacatg taattgatga ccggcacgcg ccagattaat ggccatcgga
    21181 gtacccataa tgcctaagcc aataaatccc agtttcataa ttaacctctt tttaaatgtc
    21241 gctattcctg aattcagaca acacgatccc ctgccaactt agcagcctaa aaaagcattg
    21301 tctgaatagc gttacgtttt aacggtacgg atccatccag cgtaaaccgg cttccgtggt
    21361 ggtttggggt ttatattcac acccaaccca accgttgtag tcagaatttt cgattacctt
    21421 aaagagataa tcataattaa tttctccggt ccccggttcg ccgcgatgcg gattatcggc
    21481 aatttgcagg tgaccaattt tatcagccca ttgcgtcata gtgttggtta attcaccttc
    21541 catccgctgc atgtgataaa tgtcatactg aattttcaaa ttgcagcaac caacatcatc
    21601 aatcaatttc agcgcctgcc gagttccggt gagatggaaa ccaggaatat caaaatggtt
    21661 aataggttca atcagtaata aaatatcttc tttcatcagc atattcgcgg cataacgcag
    21721 gttttctaca agcgttgcgt gaatctgttc actgctgaaa ccagccggcg ttttaccgac
    21781 cagacagtta atatttttat tacccagcgc acgggcataa cgaatcgctg ctgctacgcc
    21841 atcccgaaac tcttcttcac ggccaggaat acaggcaata ccgcgctccc ccgccgccca
    21901 gtcacccgcc ggtaaattgt gcagcgtatg ttcgagttta ttactcgcca gcacctgttt
    21961 taattcttca atgtcgtagt cataaggaaa cataaattca acgccgcgaa aaccacactg
    22021 cgcagctttc tcaaaacggg cgagaaaatc atattcacca aataacatcg ataaattagc
    22081 agagaaacgt aacattattt atctccctta ttcatagtgc atgaagcagg tttcagtcgg
    22141 tgcatccgct gcgttatcgg cgatatcttc aaattccatg acgttatcca gttcgctgcc
    22201 catcgaaata tttgtcacac gctcgagaat aacttccacg actaccggta cccgatattg
    22261 cgccattaag gctttcgcct gttcaaaggc cggtgcaata tcttccggtt tgaagacgcg
    22321 aatagcttta caacctaaac cttccgctac ttttacgtgg tcgacgccgt aaccgttcac
    22381 ttcgctggag ttgatattct cgaaagcgag ttgcacgcag tagtccatgt caaaggcgcg
    22441 ttgtgactga cgaatcaggc cgagataggc gttattcacc agcacatgga tgtacggaat
    22501 gttgaactgc gcgccaacgg ctaactcttc aatcaggaac tggaagtcga agtcaccgga
    22561 aatcgccacc acattgcgtt tcggatcagc ggcacaaacc cccagcgccg ccggaatcgt
    22621 ccagcctaac ggaccagcct gaccacagtt gatccagtgg cggtctttaa agacatgcag
    22681 catttgtgcc gcagcgattt gtgacaggcc aatggtggtg acataacaaa catcgcgacc
    22741 aaaggctttg ttcatctctt catacacgcg ctgcggtttc accggcacgt tgtcgaaatg
    22801 ggttttgcgc agcaaagtgc gtttgcgctg ctggcaatcg gcgacccatt ctttacgaca
    22861 cggcagacga cccgcttttt gcatctcctg cgccacttca accagcagtg tcagcgccgc
    22921 tttagcatca gagacaatgc cgagatccgg acacagcacg cggccaattt gcgtcggctc
    22981 aatatcgata tgaacgattt tgcgcccttc ggtgtatttc tctaccgaac cggtatgacg
    23041 gttagcaaaa cggttaccga taccaaacac catgtcggac gccagcaacg ttgcgttacc
    23101 gtaacgatgc gcggtttgca gaccgaccat cccggccatc agttcatgat cgtccgggat
    23161 acagccccag cccatcagcg ttgggatcac cggaacgctg gtcagttcag caaactgttg
    23221 taacagtgca gctgcgtcag cattgattac cccgcccccg gcaacaatca ccggacgttc
    23281 ggcctggatt aacatttcta cagctttttc gatctgcata cggctggcag caggtttgta
    23341 gaccggcagc ggttcgtaca tgtcaggatc aaactcgatt tccgcaacct gaacgtcgaa
    23401 cggtaaatcc accagtaccg gacccggacg accagaacgc atcaggtgaa atgcctgttg
    23461 cagcacgcga ggcaccagcg ccgcttcacg aactgtaacc gccattttgc tgaccggttt
    23521 agcaattgct tcaatatcta cggcctgaaa atcttcttta tgcagacggg cgcgcggtgc
    23581 ctggccggta atgcacagaa taggaatgga atcagcagaa gcggaataga gcgcggtgat
    23641 catgtccgtg cccgcaggac cggaagtccc cagacatacg ccgatattcc ctgccgttgc
    23701 gcgggtataa ccttccgcca tgtgcgaagc accttccaca tgacgcgcca gaatgtgacg
    23761 aataccgccg tgcttacgca tcgctgagta gaacggattg attgcagctc ccggaacgcc
    23821 gaaggcggtg gtgatacctt ctttctccag cacatacatt gccgcgtcaa cggctctcat
    23881 ttttgccatt ttattcctac ctctattaat tggaaaaatt ttccaacttt taaattttgt
    23941 caaaaaaacc cgctccgcag agcgggatgc gacagacact atggatgtgc tttcagtccc
    24001 aacgccgtgc tgatatcgcg ggcggtgtct ctgaccagct caccctgact gacaaaacga
    24061 tcttctgtca gtcttgatga aggcccggag atggagatag cggcaacaac gctacctaca
    24121 tcatcgtaaa ttgctgaagc tatgcaattc agacctacaa catgctcttc tttatctacg
    24181 gtatagccca gttcacgcgc ttgttccagg tccttcagca gggtgggcat atccacaagc
    24241 gtagttggcg taaactgctg caaaccggtt tgcagaatga tgctcatcaa ctcctcttcc
    24301 gccagcggat aaagcagcgc tttgcccgca ccggaagcat gcagtggcag acgactgccc
    24361 agtggcgcgc acatcctgac catcgattta cactctaact gaccaattaa taccgcttca
    24421 ttgccgttac ggatcgcgac attgaccgtt tcgccggaaa gtaacatcag gcggcgcata
    24481 aacggcccgg cgacggagag gacatcgcgg ttatggatgt acgccgcacc gacgttaaag
    24541 acacctaatc ctatatgcca ccagcctaac tgactgtcct gatagacaaa atccgctgcc
    24601 tgtaaaacct tcagcaagcg aaaggtcgtg gagagcggca aatccagatt gagagaaata
    24661 tcgctaaccg acgaacttcc cccacttttt tccagatatt gcagaatcgc aattccccgc
    24721 tctaatgcct gtgcgccctt ctgtgccaca ggctccgcct gtcctggcct gccgcgccgt
    24781 ctaacttccg tcatacctac gttaactccg acctacaagt gagcataaac aatgaacaat
    24841 tagtttatct tatgcaagtc ggttgcaggc gtcacgcaaa acagagttcc tgttcaggaa
    24901 tactttcaac atcacagttg tcactgccgc cgcgatcgat ggtcagaaaa tcggtgacgc
    24961 gctgccaggc gaaaagtggg tgatgccaga cgttacgatg gtaattcact ccctgttcgc
    25021 cgttggtaat aaacgcccgc agggttgaca ggtctggttt gtcgtcacct aacgctacga
    25081 ccaccacaaa cacctcacct ttcatcggga taaaggcctg agtacccagc ggatgacgtt
    25141 cgagttcgtg aatggtcagc ggcagattcg ccggttgcgc tcggttaatg ctgataagcg
    25201 tacggtcttg ctcaagaatc tcaaccagcg ccaaatcgtg gtaacgctcc accaggccat
    25261 tgttaatatg gaaaaaatcc cgttgctgcg tttcgattac gtcgccataa gcactaaagg
    25321 cttcctgact taacggtaat acctgaagtt tcatcttttt gtccttgtaa cataattccg
    25381 ttcaaccagc gccgaccggt cagcttggtt tcaataacag gcaatctaaa accttaagag
    25441 aacaacatca acacaaaact tggaatattt ttccaaaact agatttagtc cacaaattga
    25501 acagggttat catgtaaaaa tactgataaa tcagaggata agaaaatcac gcaacttgcg
    25561 gatttaaaag gggatacctg aagatgttcg atccagaaac cttgcggact ttcattgcgg
    25621 ttgctgaaac aggaagtttt tcaaaagcgg cagaacgatt atgtaaaacc acggcgacga
    25681 tcagttatcg cattaaactt ctggaagaga ataccggagt ggcgctgttt tttcgcacga
    25741 ctcgtagcgt aacgttgaca gcggctggcg agcatctact ttcccaggcc agagactggc
    25801 tgagctggct ggaaagtatg cccagcgagc tgcaacaggt gaatgatggc gtggaacgcc
    25861 aggtgaatat tgtcatcaac aacctgctct acaaccccca ggccgtcgcc cagttgctgg
    25921 cgtggctgaa tgaacgttac ccctttaccc agtttcacat ctcccgacaa atctatatgg
    25981 gcgtctggga ctcgctattg tacgaaggtt tttcactggc tatcggcgtc acgggaaccg
    26041 aagcgctggc aaataccttt agtcttgatc ccttaggatc ggtgcaatgg cgatttgtca
    26101 tggcggcgga tcatccgctg gcgaacgttg aagagccgct aacagaagcg cagttgcggc
    26161 gctttccggc ggtcaatatt gaagacagcg cccgcacctt aaccaaacgc gtcgcctggc
    26221 gattgccagg gcaaaaagag attattgttc ctgatatgga aacgaaaatc gccgcccatc
    26281 tggcgggcgt tggcattggt tttttgccaa aatcgctttg ccagtcaatg atcgataatc
    26341 aacaactggt cagccgggta atcccaacga tgcgccctcc ttcgccattg agtctggcat
    26401 ggcgcaaatt tggcagcggc aaagcggtag aagatattgt gaccttgttt acccagcgca
    26461 ggccggaaat cagcggattt ttagaaattt tcggcaaccc acgcagttaa aaatcaagtt
    26521 tccttgcgcg ttggcgcgta aaatagcgcg caagctgatt tgaggttcac ttttaactat
    26581 gcaagagaga cacacggaac aggactatcg tgccctgctg attgctgata cgcccattat
    26641 tgatgttcgc gcccctatcg agtttgagca aggcgcaatg cccgccgcta tcaatctgcc
    26701 gttaatgaat aacgatgaac gcgccgccgt tggcacctgc tataaacagc aaggctcaga
    26761 cgcagcgctg gcgctgggac ataaactggt tgcgggtgaa attcgtcagc agcgcatgga
    26821 cgcctggcgg gcagcctgcc tgcaaaatcc gcaaggtatt ctctgctgcg cccgtggcgg
    26881 tcagcgctca catattgtgc aaagctggtt gcatgcagcg gggattgatt atccgctggt
    26941 ggaaggcggt tataaggcac tgcgccagac cgcgattcag gcgactattg aactggcaca
    27001 aaaaccgata gtgctgattg gcggttgtac cggcagcggt aaaacgctgt tagtgcagca
    27061 acagccgaac ggtgttgatc tggaagggtt ggcgcgtcat cgcggttcgg cgtttggtcg
    27121 cacgttacaa ccacaactta gccaggcgag ttttgaaaac ctgctggctg ccgaaatgct
    27181 aaaaaccgac gcccgtcaga atttgcgcct gtgggtgctg gaagacgaaa gccggatgat
    27241 cggttcgaat cacctgccgg aatgcctgcg cgagcgaatg actcaggcgg cgattgcggt
    27301 ggtagaagat ccgtttgaga tccgtcttga gcgcctgaac gaagagtatt tcttgcgtat
    27361 gcatcatgat tttacccacg cgtacggcga cgaacagggc tggcaggaat attgcgaata
    27421 cctgcatcac ggactttcgg ctattaagcg tcggctgggg ctacagcgat ataacgaact
    27481 ggctgcaagg ctggatgcag cactgacaac gcaactcacc accggcagca ccgacggtca
    27541 tctggcctgg ctggtgccgt tacttgaaga atattacgac ccgatgtatc gctatcagct
    27601 tgagaaaaaa gcggaaaaag ttgtctttcg cggtgagtgg gcggaagtgg cggaatgggt
    27661 taaggcgcag taatgaggga cgtatttgta ttagtcgcga cttgatctga cacatggcct
    27721 tgaaaggttg agagttaccg gttttgatat gagtgtcgaa tccttatcca aaacaagagg
    27781 taactctcat gcttcatact gccaatcccg tcatcaaaca caaagccggt ttgctcaatc
    27841 tggctgaaga actcagcaac gtatcaaaag cctgtaaaat catgggcgtt tcgcgtgata
    27901 cgttttatcg ttaccgcgaa ctggtcgctg aaggcggtgt ggatgcgcag attaatcgta
    27961 gtcgccgcgc tcctaacctt aagaaccgta ccgatgaggc aactgaacag gctgttgttg
    28021 attacgccgt cgctttcccg acacatggtc agcaccgggc cagcaacgaa ctgcgtaaac
    28081 agggggtttt tatctccgac agtggtgtcc gttctgtctg gttactccat aaccttgaga
    28141 atctcaaaag acgatactga acaaatttta tcaggtgacg ttccgtaaaa agttgtatgg
    28201 agttctcgat gcagtcggcg cgactatcag tacccccgct gaaccctgga ctatcgggcc
    28261 gccgtactgg ggcatgtcac cctggcgtgc cgctggtttt ccgctaatgg tttatttcct
    28321 ctttctttcc cggcaagaat ttattttaat ccggaaggat tatttatttt cagataggga
    28381 ctcacattaa acacgtaaca ttttaattaa tgcatggctg aatatgtatt acacaatgtc
    28441 agatatcgta aaagaccagt gcaattctat gtaaactctc tgactgttca ttttatttgt
    28501 tgtttcaggg tcggaactca ccctcaataa acaactgaat acgagtagat attcatcgtc
    28561 tgagctatat ggctttacac aatagccgac atgtcttttc tcagtcagga attttacttt
    28621 tcctcactac gaagttgacg acttcgatat gggatagact cttaattcaa gcaattactc
    28681 tgcacaagca tcaggcaatc cagctccaat atttgttata aaataatgcg taacattaat
    28741 ttcaccaaca aaaaaaggtt tacaccatcc ccttgccctg gtatcttacc atatttacac
    28801 cacacttttt ttgtatctgg atcattcttt gatacgattt tcgccacttg ttccctggcc
    28861 ctcactgtca atgttttctt aaacctcccc ccatcatcat ttgttaggga aaaataagaa
    28921 aaaaataatc atcttctaca tttgtgtata ttgatatatc caccgctacg tgcggaaaaa
    28981 aatctgggag ccatgtaaaa actccatcat tttctggt
//
LOCUS       DAZFAB010000051        28279 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZFAB010000051 DAZFAB010000000
VERSION     DAZFAB010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28279
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.051"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..209)
                     /locus_tag="ACHJWA_002586"
     CDS             complement(<1..209)
                     /locus_tag="ACHJWA_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO4309161.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHJWA_002587"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHJWA_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFO4309162.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHJWA_002588"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHJWA_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFO4309163.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHJWA_002589"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHJWA_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFO4309164.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHJWA_002590"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHJWA_002590"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFO4309165.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSGQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHJWA_002591"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHJWA_002591"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFO4309166.1"
                     /translation="MKRIAFVFSTVPHGTAAGREGLDALLATSALTDELAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLDLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHJWA_002592"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHJWA_002592"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFO4309167.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANHLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDEMEAGRLGLASSN
                     LQQGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /gene="yheO"
                     /locus_tag="ACHJWA_002593"
     CDS             complement(4598..5320)
                     /gene="yheO"
                     /locus_tag="ACHJWA_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO4309168.1"
                     /translation="MSRSLLTNETSELDLLDQRPFEQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHJWA_002594"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHJWA_002594"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO4309169.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /gene="slyX"
                     /locus_tag="ACHJWA_002595"
     CDS             6520..6738
                     /gene="slyX"
                     /locus_tag="ACHJWA_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFO4309170.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHJWA_002596"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHJWA_002596"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO4309171.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /gene="yheV"
                     /locus_tag="ACHJWA_002597"
     CDS             complement(7472..7672)
                     /gene="yheV"
                     /locus_tag="ACHJWA_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFO4309172.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7709..9487)
                     /gene="kefB"
                     /locus_tag="ACHJWA_002598"
     CDS             complement(7709..9487)
                     /gene="kefB"
                     /locus_tag="ACHJWA_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFO4309173.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERR"
     gene            complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="ACHJWA_002599"
     CDS             complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="ACHJWA_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFO4309174.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10169..12082
                     /gene="yheS"
                     /locus_tag="ACHJWA_002600"
     CDS             10169..12082
                     /gene="yheS"
                     /locus_tag="ACHJWA_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO4309175.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12082..13104
                     /gene="yheT"
                     /locus_tag="ACHJWA_002601"
     CDS             12082..13104
                     /gene="yheT"
                     /locus_tag="ACHJWA_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO4309176.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRKVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQAQHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13098..13316
                     /gene="yheU"
                     /locus_tag="ACHJWA_002602"
     CDS             13098..13316
                     /gene="yheU"
                     /locus_tag="ACHJWA_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFO4309177.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13370..14239
                     /gene="prkB"
                     /locus_tag="ACHJWA_002603"
     CDS             13370..14239
                     /gene="prkB"
                     /locus_tag="ACHJWA_002603"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFO4309178.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAIELIML
                     PLVQRLMEGKKIE"
     gene            complement(14294..14698)
                     /gene="yhfA"
                     /locus_tag="ACHJWA_002604"
     CDS             complement(14294..14698)
                     /gene="yhfA"
                     /locus_tag="ACHJWA_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFO4309179.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15000..15632
                     /gene="crp"
                     /locus_tag="ACHJWA_002605"
     CDS             15000..15632
                     /gene="crp"
                     /locus_tag="ACHJWA_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFO4309180.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15683..17773
                     /gene="yhfK"
                     /locus_tag="ACHJWA_002606"
     CDS             15683..17773
                     /gene="yhfK"
                     /locus_tag="ACHJWA_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFO4309181.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17840..19060)
                     /gene="argD"
                     /locus_tag="ACHJWA_002607"
                     /gene_synonym="dapC"
     CDS             complement(17840..19060)
                     /gene="argD"
                     /locus_tag="ACHJWA_002607"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFO4309182.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPSDIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGQARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="ACHJWA_002608"
     CDS             complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="ACHJWA_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFO4309183.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKVVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPDSLPACFEVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19741..20343)
                     /gene="fic"
                     /locus_tag="ACHJWA_002609"
     CDS             complement(19741..20343)
                     /gene="fic"
                     /locus_tag="ACHJWA_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFO4309184.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKTKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20333..20500)
                     /gene="yhfG"
                     /locus_tag="ACHJWA_002610"
     CDS             complement(20333..20500)
                     /gene="yhfG"
                     /locus_tag="ACHJWA_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFO4309185.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRSHYER"
     gene            complement(20605..21177)
                     /gene="ppiA"
                     /locus_tag="ACHJWA_002611"
                     /gene_synonym="rotA"
     CDS             complement(20605..21177)
                     /gene="ppiA"
                     /locus_tag="ACHJWA_002611"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFO4309186.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21448..22629
                     /gene="tsgA"
                     /locus_tag="ACHJWA_002612"
     CDS             21448..22629
                     /gene="tsgA"
                     /locus_tag="ACHJWA_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFO4309187.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVAKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22891..25434
                     /gene="nirB"
                     /locus_tag="ACHJWA_002613"
     CDS             22891..25434
                     /gene="nirB"
                     /locus_tag="ACHJWA_002613"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFO4309188.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25431..25757
                     /gene="nirD"
                     /locus_tag="ACHJWA_002614"
     CDS             25431..25757
                     /gene="nirD"
                     /locus_tag="ACHJWA_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFO4309189.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25883..26689
                     /gene="nirC"
                     /locus_tag="ACHJWA_002615"
     CDS             25883..26689
                     /gene="nirC"
                     /locus_tag="ACHJWA_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFO4309190.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGTISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26708..28081
                     /gene="cysG"
                     /locus_tag="ACHJWA_002616"
     CDS             26708..28081
                     /gene="cysG"
                     /locus_tag="ACHJWA_002616"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFO4309191.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         6838 a         7594 c         7138 g         6709 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 aatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcctgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccatacgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgagc acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggccggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcgaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtcgccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggggtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagatcca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctggca gtagctgaaa
     4081 aacgccatca gcaataaaga agacggccag ctcgtcagtt aacgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgcc cggcagcggt accatgaggc acagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tccctgctga aggtttgacg aagccagccc cagtcttccg
     4321 gcctccattt catcaacaac gccacggcgt aatgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca agtggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctggaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaagg tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctgctcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 accagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagagagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcacgacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagtggaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttcttttag agagttttcc
     6121 atgtaacgac ccagcgaggc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gctgctttgc tgtcagcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaagg caacggccat tgtggtcgcc agcagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctact ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tgaccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccgc aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccacgacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcggt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc accaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gcgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tggtgtccgc
     7561 acttaacgca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcaa taaaacgttt tcggattgcc atctttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct agcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcggcat
     7801 gcattgggat gagctctcgc agcatccgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc ataccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatctacct gcgtggcatc accgtaataa actttgtagc
     8161 catatttgcg catcaggtta acggcgctga tatcccgctc cagcacagta atgcgcattt
     8221 tatttgccat caacaaacga ccaatcacct gcccaaaacg tccaaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcagcgg cgtggtcatc atggaaagcg
     8401 tcaccgtcac cagcaacagc gccatctggt cgccctggaa taagcgttgt gaagaagcgg
     8461 tagaaaagag gacaaaggca aactcaccgc cctgactcaa cacgccagca aactgcatcc
     8521 gctctgagct acgcacgcca tacaatcgcg ccagcagata cagcacgaga attttcaccg
     8581 ccaccagcac aaccacgctt atcactaccc acaacagatg ggtataaagc accccgaggt
     8641 tgagtgacat cccgacagag ataaagaaca aaccgagcag caagccttta aagggatcga
     8701 tagccgtttc cagttcatgg cgatattcac tttccgccag cagcacgcct gcaataaacg
     8761 taccgagcgc catcgacagc cccaacgcat ccataaacaa tgcggaaccc aataccagca
     8821 gcagcgtcgc ggcggtgaac acttcccgca cgccagaagc tgcaataaaa cggaataccg
     8881 gacgcaataa atagcgcccg ccaatcagca tgccgacaaa cgccagcacc ttcatgccga
     8941 tcttcatcca gtcgaaatgt tcgtctgccg aacccgccaa caacggcact aacgccagtg
     9001 ccgggatcac cgccagatcc tgaaacagca gtaccgaaaa cccgagctgc ccggattcgc
     9061 tgcgattcat ccctttctca cgcatcaatt gcaacgccat tgcggtcgaa gacatcgcaa
     9121 ggccaatgcc accgaccacc gccgcctgcc aggcgaaatc cgtcagcatc aataatcccg
     9181 ccagcaacgc cgcgcttaac agcacctgtg ccgcgcctac gccaaaaatc gaacgccgca
     9241 gttgccaaag cttggaggga ttcaactcaa ggccgatgat aaacatcagg aacaccacgc
     9301 cgagttccga aaagtggagg atctcatcga cgtcgctaat aaaccccagc ccccacgggc
     9361 caattgcaat ccctgccagc aaatatccca acacagcgcc aatacccagc cgcgatgcca
     9421 acggcaccgc agccaccgcc gcgaagagaa acagcactcc tgcaagtaaa aaatcggaac
     9481 cttccatcag cggcctcctg gagacagcgg atttgccagc cagtcaccgt aggctctggc
     9541 gtggctcgcc agctcctgtg cgctttgccg tctcgcccag taaataatga tgggacttaa
     9601 ccaatgcatc cggcacatgc ccgccgccag ttcaaagggg cgcaggacat cgctcatcgg
     9661 gtaacgattc aacgcgtcat aacggtaagc actttccggc tcgccggtgg taatcacact
     9721 acgccagtac tttcccgcca gttggtttcc tcccggcccg ctggcaaaac cacggcttaa
     9781 tacccggtcc agccactctt tcagtagcgc cgggcagcta taggtataaa gaggatgctg
     9841 aaagacaatc acctcgtgct cgcgcagtaa tgcctgctca cgggggatat caataaaaaa
     9901 atcgggatag tgcgcgtaaa ggtcgtgcac ggtaacattg ctgagctgcg tggccggttt
     9961 aagcagtacc cggtttgcca ccgagtcctg agattccgga tgggcataca gcagcaaaac
    10021 tttcgctggc tgagacatca tccccctccc ggtatggttt ttgtgtatag tcgctgtttt
    10081 gggctaccat tgcgcccggt gcggcagctc gcccatacat tacattatca taatgataag
    10141 ttaacatagt ctgaacatac ggcaccttat gattgttttc tcctcgttac aaattcgtcg
    10201 cggcgtgcgc gtcctgctgg ataatgccac cgccaccatc aaccccgggc agaaagtcgg
    10261 cctggtgggt aaaaacggct gtggtaaatc taccctgctg gcattgctga aaaatgaaat
    10321 cagcgccgac ggcggcagct acacctttcc gggaagctgg caactggcgt gggtgaatca
    10381 ggaaacgccg gcgttaccgc aagcggcgct ggaatatgtc attgacggcg accgtgaata
    10441 tcgtcaactg gaagcgcagc tacacgacgc caacgaacgt aacgacgggc acgccattgc
    10501 gaccattcat ggcaagctgg atgctattga cgcatggagt attcgctccc gtgccgccag
    10561 cctgctgcac ggcctcggtt tcagcaatga acaactggag cgcccggtaa gtgatttctc
    10621 tggtggctgg cgtatgcgtc ttaaccttgc ccaggcgctg atttgccgtt cagacttgct
    10681 gctgctcgac gaaccgacta accacctcga tctcgatgcc gttatctggc tggaaaaatg
    10741 gctgaagagc tatcagggca cgctgatcct gatctctcac gaccgcgact tcctcgatcc
    10801 gatcgtcgat aaaattattc atatcgaaca acaaagcatg ttcgagtaca ccggcaacta
    10861 cagttcgttt gaagtacagc gcgccacccg tctggcgcag caacaagcga tgtacgaaag
    10921 ccagcaggaa cgcgtagcgc atctgcaaag ttatatcgac cgtttccgtg ccaaagccac
    10981 caaagcgaag caggcccaga gccgcattaa gatgctcgag cgtatggagc tgattgcccc
    11041 cgcgcacgtc gacaacccgt tccgctttag cttccgcgcg ccggaaagcc tgccaaatcc
    11101 gttactgaag atggaaaagg tcagcgcagg ctatggcgat cgcattattc tcgactcgat
    11161 taaactgaat ctggtccccg gttcgcgcat tggtctgtta ggccgcaacg gcgcgggtaa
    11221 atcgacatta atcaaactgt tagccggtga acttgcgcca gtcagtggtg agataggcct
    11281 ggcaaaaggg atcaagctcg gctacttcgc ccagcatcaa cttgaatacc tgcgcgccga
    11341 cgaatcgcct attcaacatc tggcacgttt agcgccgcag gaactggagc aaaaactgcg
    11401 tgactacctc ggcggctttg gtttccaggg cgataaagta accgaagaaa cgcgccgctt
    11461 ctccggtggg gaaaaagccc gcctggtgct ggcattaatc gtctggcagc gtccgaatct
    11521 gctgctgctc gacgaaccga ctaaccacct tgaccttgat atgcgtcagg cactcaccga
    11581 agcattaatc gagtttgaag gcgcgctggt tgtcgtttcg cacgaccgtc atttgctgcg
    11641 ttccaccact gacgatctct acctggttca cgatcgtaaa gtcgaaccgt tcgacggcga
    11701 tctggaagat tatcaacagt ggttgagcga cgtacaaaag caggaaaacc agaccgacga
    11761 agcgccaaaa gagaacgcga acagcgccca ggcacgtaaa gatcagaagc gtcgggaagc
    11821 ggagctgcgt gcgcaaaccc agccactgcg taaagagatt gcccgtctgg aaaaagagat
    11881 ggagaagctg aacgcgcaac tggcgcaggc agaagagaaa ctcggcgaca gcgaactgta
    11941 tgaccagagc cgtaaagcgg agttgaccgc ctgcctgcaa cagcaagcca gcgccaaatc
    12001 cggcctggaa gagtgcgaaa tggcatggct ggaagcccag gagcagcttg agcagatgct
    12061 gctggaaggc caaagcaact gatggcgcag ataacgacga ccgatgccaa tgaattcagc
    12121 agcagtgctg aattcacccc tatgcgcggc tttagcaatt gtcatctgca aaccatgctg
    12181 ccgcgtctgt ttcgtcgcaa ggtgaaattc accccgtact ggcagcgact ggagttgccc
    12241 gacggcgatt ttgtcgatct cgcgtggagt gaagcccctg cacaggcgca acataaacct
    12301 cgtctggtgg tgtttcacgg gctggaaggc agtctcaata gcccttacgc ccacggtctg
    12361 gttgaggcgg cgcaaaagcg cggctggctg ggcgtggtga tgcattttcg cggatgcagc
    12421 ggtgaaccaa accgtatgca ccgcatttac cattcgggcg aaaccgaaga cgccagttgg
    12481 tttttacgct ggctgcaacg cgagtttggc catgcgccaa cggctgccgt cggctattcg
    12541 ctcggcggta atatgctggc ctgtttgctg gcaaaagaag gcaatgatct cccggttgat
    12601 gcggcggtta ttgtctctgc gccgtttatg ctggaagcct gtagctatca tatggaaaag
    12661 ggcttttccc gcgtttatca gcgttacttg ctgaacctgt taaaagccaa tgccgcgcgc
    12721 aagctggcag cctaccccgg aacactgccg attaatctcg cgcagttaaa atcggtacgt
    12781 cgcatccgtg aatttgacga tctcatcacc gccagaattc acggctacgc tgacgctatc
    12841 gactattatc gtcagtgtag cgccatgccg atgctgaacc ggatcgccaa accgacgctg
    12901 attattcacg ccaaagacga tccgtttatg gatcatcagg tgatcccgaa accggaaagt
    12961 ctccccccgc aggtggagta tcaactgact gaacatggcg gtcatgttgg ctttattggc
    13021 ggtacattac ttcatccgca aatgtggctg gagtcacgca ttcctgactg gttaacaacg
    13081 tatctggagg cgaaatcatg ttgattccgt ggcaagacct ctcccccgaa acgctggaaa
    13141 atttaattga aagctttgtg ttacgtgaag gcaccgatta tggtgaacat gagcgtacac
    13201 ttgaacagaa agtcgccgac gtcaaacgcc agctacagtg cggagaagcg gtgctggtat
    13261 ggtcggagct gcacgaaacg gtcaatatca tgccgcgcag tcagtttcgc gaataacctg
    13321 caaccgtcgg cggacgagat aataatgata gctaagtcat ggagttacta tgtctgccaa
    13381 acatccggtc attgcggtaa caggatccag cggcgcgggg accaccacca ccagcctcgc
    13441 gtttcgtaaa atattcgcgc agttaaatct gcatgcagcc gaggtggaag gcgacagttt
    13501 tcaccgttac acccgcccgg aaatggacat ggcgatccgc aaagcgcgcg acgccgggcg
    13561 gcatatcagc tacttcggcc ccgaagcgaa cgacttcggc ctgctggaac aaaccttcat
    13621 tgaatacggt cagagcggca aagggaaatc tcgcaaatat ctgcatacct acgatgaagc
    13681 cgtaccgtgg aatcaggtac cgggaacatt caccccctgg caacctttac cagaacccac
    13741 tgatgtactg ttttatgaag gtttacacgg cggcgtagtc acgccacagc ataacgttgc
    13801 gcagcatgtg gacttactgg tcggcgtggt acctatcgtt aaccttgagt ggattcaaaa
    13861 actgatccgc gacaccagcg agcgcgggca ctcacgagaa gcagtgatgg attcagtagt
    13921 gcgttcaatg gaagactata tcaactacat cacaccgcag ttttcccgca cccatcttaa
    13981 cttccagcgc gttcccaccg tcgacacttc aaacccgttc gcggcaaaag gtatcccgtc
    14041 gctggatgaa agctttgtgg tgatccattt tcgtaatctg gaagggatcg atttcccctg
    14101 gctgctggcg atgttgcaag gctcattcat ttcccacatc aatacgttag tggtgcccgg
    14161 cggcaaaatg ggtctggcaa ttgaattaat tatgctgccg ctggtgcaac gattgatgga
    14221 aggaaagaaa atcgagtaac tctgctatta cgccggataa aatactatcc ggcttcacaa
    14281 cgggatagtt aaatcacgcg gcaaccactt catacgagtg agtaatattc accgcttttt
    14341 ccagcatcaa cgccactgaa caatatttct cagcagagag atcaaccgca cgcgcaaccg
    14401 ctgcgtcttt caggtcgcga ccggtgacga taaaatgcag attaatgtgc gtaaacaggc
    14461 gtggtgcctc ttcgcggcgt tcagaggtca attttacttc acaatcgacc acatcctgac
    14521 gccctttttg caggatcgaa accacatcga tggcactgca accacccgcc gccatcaaca
    14581 ccatttccat cggacttggt gctttatcgc ctgagttgcc gtccattaaa atctggtgac
    14641 cagaggcaga ttcgcccaga aaagttaacc cttcgaccca cttcactcgc gcttgcattt
    14701 ttgctactcc actgcgtcaa ttttcctgac agagtacgcg tactaaccaa atcgcgcaac
    14761 ggaaggcgac ctgggtcatg ctgaagcgag acaccaggag acacaaagcg aaagctatgc
    14821 taaaacagtc aggatgctac agtaatacat tgatgtactg catgtatgca aaggacgtca
    14881 cattaccgtg cagtacagtt gatagcccct tcccaggtag cgggaagcat atttcggcaa
    14941 tccagagaca gcggcgttat ctggctctgg agaaagctta taacagagga taaccgcgca
    15001 tggtgcttgg caaaccgcaa acagacccga ctctcgaatg gttcttgtct cattgccaca
    15061 ttcataagta cccatccaag agcacgctta ttcaccaggg tgaaaaagcg gaaacgctgt
    15121 actacatcgt taaaggctct gtggctgtgc tgatcaaaga cgaagagggt aaagaaatga
    15181 tcctctccta tctgaatcag ggtgatttta ttggcgaact gggcctgttt gaagagggcc
    15241 aggaacgtag cgcatgggta cgtgcgaaaa ccgcctgtga agtggctgaa atttcgtaca
    15301 aaaaatttcg ccaattgatt caggtaaacc cggacattct gatgcgtctg tctgcacaga
    15361 tggcgcgtcg tctgcaagtc acttcagaga aagtgggcaa cctggcgttc ctcgacgtga
    15421 cgggccgcat tgcacagact ctgctcaatc tggcaaaaca accagacgct atgactcacc
    15481 cggacggtat gcaaatcaaa attacccgtc aggaaatcgg tcagattgtc ggctgttctc
    15541 gtgaaaccgt gggacgcatt ctgaagatgc ttgaagatca gaacctgatc tccgcacacg
    15601 gtaaaaccat cgtcgtttac ggcactcgtt aatcccgtcg gagtggcgcg ttacctggta
    15661 gcgcgccatt ttgtttcccc cgatgtggcg cagactgatt tatcaccccg atattaacta
    15721 tgcactgcga caaacgctgg tgctatgttt gcccgtggcc gttgggttaa tgcttggcga
    15781 attacgattc ggtctgctct tctctctcgt tcctgcctgt tgcaatattg cgggccttga
    15841 tacgcctcat aaacgttttt tcaaacgctt aatcattggt gcgtcgctgt ttgccacctg
    15901 tagcttactg acacagctgc tactggcaaa agatgttccc ctgccttttt tgctgaccgg
    15961 attaacgctg gtacttggcg tcactgctga gttggggcca ttgcacgcaa aattgcttcc
    16021 cgcatcgctg ctggcagcca tttttaccct cagtctggcg ggatacatgc cggtctggga
    16081 accgttgctc atctatgcgt tgggcactct ctggtacgga ttgtttaact ggttttggtt
    16141 ctggatctgg cgcgaacaac cgctgcgcga gtcactaagt ctgctgtacc gtgaactggc
    16201 agattattgt gaagccaaat acagcctgct tacccagcac accgaccctg aaaaagcgct
    16261 gccgccgctg ctggtgcgcc agcaaaaagc ggtcgatcta attacccagt gctatcagca
    16321 aatgcatatg ctttccgcgc aaaataatac tgactacaag cggatgctgc gtattttcca
    16381 ggaggcgctg gatttacagg aacatatttc ggtcagtttg catcagccgg aagaggtgca
    16441 aaagctggtc gagcgtagcc atgcggaaga agttatccgc tggaatgcgc aaaccgtcgc
    16501 cgctcgcctg cgcgtgctgg ctgatgacat tctttaccat cgcctgccga cgcgttttac
    16561 gatggaaaag caaattggcg cactggaaaa aatcgcccgc cagcatccgg ataatccggt
    16621 tgggcaattc tgctattggc acttcagccg catcgcccgc gtgctgcgca cccaaaaacc
    16681 gctctatgcc cgtgacttac tggccgataa acagcggcga atgccgttac ttccggcgct
    16741 gaaaagttat ttgtcactaa agtctccggc gctacgcaat gccggacgac tcagtgtgat
    16801 gttaagcgtt gccagcttga tgggcaccgc gctgcatctg ccgaagtcgt actggatcct
    16861 gatgacggta ttgctggtga cgcaaaatgg ctatggcgca acccgtctga ggattgtgaa
    16921 tcgctccgtg ggaaccgtgg tcgggttaat cattgcgggc gtggcgctgc actttaaaat
    16981 tcccgaaggt tacaccctga ccttgatgct gattaccacc cttgccagct acctgatatt
    17041 gcgcaaaaac tacggctggg cgacggtcgg ttttactatt accgcagtgt ataccctgca
    17101 actgttgtgg ctgaacggtg agcagtacat ccttccgcgt cttatcgata ccattattgg
    17161 ttgtttaatt gctttcggtg gtaccgtctg gctgtggccg cagtggcaga gcgggttatt
    17221 gcgtaaaaac gcccatgacg ctttagaagc ctatcaggaa gcgattcgct tgattctgag
    17281 cgaggatccg caaccaacgc cactggcctg gcagcgaatg cgggtaaatc aggcgcataa
    17341 cactctgtat aactcattga atcaggcgat gcaggaacca gcgttcaaca gccattatct
    17401 ggcagatatg aaactgtggg taacgcacag tcagtttatt gttgaacata ttaatgccat
    17461 gaccacgctg gcgcgggaac accgggcatt gccacctgaa ctggcacaag agtatttaca
    17521 gtcttgtgaa atcgccattc agcgttgtca gcagcgactg gagtatgacg aaccgggtag
    17581 ttctggcgat gccaatatca tggatgcgcc ggagatgcag ccgcacgaag gcgcggcagg
    17641 tacgctggag cagcatttac agcgggttat tggtcatctg aacaccatgc acaccatttc
    17701 gtcgatggca tggcgtcagc gaccgcatca cgggatttgg ctgagtcgca agttgcggga
    17761 ttcgaaggcg taatgcaggc taaaccatcg gataaggcat gcacgccgca tccgacatct
    17821 tttgcctgat gcgtcttatt tacgccccaa ccaccttcgc caccgcgtgg gcgaaacgtt
    17881 gcatcccttc atcgatatcc gcatcttcca ccaccagcga cggcgcaaaa cgcatcacat
    17941 ccggtccggc attcagcacc attacgccag cctctgcgcc cgcatacagg aaatcacgcg
    18001 cctgaccttt gtactgtggt ttcagctccg cgccaattaa cagccccata ccgcgaatat
    18061 cgctaaacac atcgtactgc tgatcgatct tctgcaaatg gtcaacaaaa cgctggcgtt
    18121 tcgcctgaat gccttccagc acttcagggg tattaatgat atcaaacgcc gcgcccgcta
    18181 ctgcacaggc cagaggatta ccgccgtagg tggaaccgtg agaaccagga tgaaacgcag
    18241 aagcaatttc cgccgtggtc agcatggcgc taatcgggaa gccgccgcct aacgctttcg
    18301 cagaagtcag aatatccggc gtcacgccgt agtgcatgta agcaaacaaa tcgccagtcc
    18361 gccccatccc gcactgtact tcatcaaaca ccaataatgc ctgatgttga tcgcacaact
    18421 cacgcaagcc ctgcaaaaac tctggcgtcg ctgccgtcac accgccctcg ccctggatcg
    18481 gctcaaccac caccgcacag gtgtgatcat ccatcaccgc tttcactgcg tggagatcgt
    18541 taaagggaac gtggatgatg tctgatggtt ttggcccaaa gccgtcggaa tattttggct
    18601 gcccacccac cgaaacggta aacagcgagc gaccatgaaa agcgttatgg aaggcaataa
    18661 ttttggtttt gaacgggcta tgacgcacgc aggcgtaatg gcgtgccagt ttaaaggcgg
    18721 tttcgttagc ttccgtgccg gagttcataa acaccacgcg ttcggcaaac gtcgcctcaa
    18781 tcagtttacg cccaagacgc agcgccggtt cattggtgaa aacgttactg atatgccaca
    18841 gagtttcgcc ctgggttttt aacgcgttca ccagcgcagg atggcaatgg cccaacgccg
    18901 taactgcaat gccacccgcg aaatcgacat actccttgcc ttgctgatcc cagattcggc
    18961 tgccctgacc ttttaccgga atgaactctg ccggagcata aatcggcagg atcacttcat
    19021 cgaaagtcgc gcgtgtaatt gctgtttgtt caattgccat ctcatgatca ccctgttatg
    19081 cataaataaa tgtgaaatta taatcacaaa atatgcataa aaaatcattg aatggcaatc
    19141 agaaatcagc gatgcaggaa attagccagc agttgatgtc cttgttcgct aagaatactt
    19201 tctggatgga actgcacacc ttccagatcc cactggcgat ggcgaatccc cataatctcg
    19261 cgggtttcgc tccaggccgt cacttcaaag cacgctggta acgagtcagg ttccactacc
    19321 agcgaatgat agcgtgtcac ggtaagtgga tttgccagcc cccgaaatac gccctcaccg
    19381 ttatgtgtaa tcggcgaggt tttgccgtgc atgacctttg cggcgcgcac aactttaccg
    19441 ccaaatgcct gcgccattgc ctgatgaccg aggcagacgc caagaatcgg caagcgcccg
    19501 gcatagtggc gaataacgtc aagggagatc ccggcttcat ctggcgtaca ggggccgggt
    19561 gagatgacaa ctttttgtgg tttaagggcg tcgatatccg ccagcgtcaa cgcatcgttg
    19621 cgcttaacca gcacatccgc ccccagttca caaaagtact ggtagaggtt ccaggtaaaa
    19681 gaatcgtagt tatctataag caggatcatg gcggctccgg tacgaaagaa ccgcgctatt
    19741 ttactcagat tccccggctt cgcttaccac tttgctaaat atcatctgca acgcggtgag
    19801 atcccccatt gccccactct gatttgcctg attccaggcc tctttttcga taccctgcca
    19861 gctcagttga tatccggcat gaatcgccag ttgctcgaag aagatccgct gtgccagacc
    19921 acttcccacc cggaagggat gcagcacgtt gatttcacaa tagtaatgcg ccagccgctc
    19981 gacgaacttc gttttctcca ggccaaccag ataaccttct tcctccagat cctgcatcag
    20041 ggcattgccc tctttttcga tataagcaaa gtggcagaac ggcgtatcgc cctgataaat
    20101 atcaacttca cgcagttgcc ctgcccagtc gaaaatatcc tgatacagct ggcgatggat
    20161 agtgcgcaaa tgcggtaaac cgcgcaccag cggaccaagc tcaatggtcg cagcacgcag
    20221 cgccgtcatt tcgtaagcgg cctgttccag ccgttgttgc tggcggatgt tcagccggtt
    20281 acgcatgata tcaaggccag gataaagata cggatcgcgc ccttcgccga atttatcgct
    20341 catagtgact cctcagctct tcaaggcgcg ctaaagcctc tgcggcagta agagtgacta
    20401 aaggcatctc gacgccttca agacggcgac tggcctgaaa attacgatta cgctgaagct
    20461 cccagagacg ggacttttgc ttatcggtga gtttcttcac ttgatgcctc cctgaacgtg
    20521 tccatttgcc acaagtataa gcggcaaatc cgggttacgc cgggagagca gaagcgcggg
    20581 caagattgcc cgcgcgagaa atcattacgg caggacttta gcggaaagga taactaccgg
    20641 ttttgacggc acattctggt acggaccaac gtcatgagtc ggcacctggg aaatcttatc
    20701 ggcaacgtcc atgcctttca ccactttacc aaataccgcg taaccgaaat cacgctgacc
    20761 atggtcaagg aaggcgttat cggcaacgtt gataaagaac tggctggtgg cgctgtcttt
    20821 gtcagcggta cgcgccatcg cgatggtgcc acgcgtgttg cgaaggccgt tatcggcttc
    20881 atttttgatt ggcgggtttg gttttttctg ctgcatctgc tcggtgaaac cgccgccctg
    20941 aatcataaag ccaggaatga cgcggtgaaa ggtagtgttg ttataaaaac cgctgttcac
    21001 ataatcgaca aagttttgca cagacactgg cgctttttgt ttatccagct ccagttcgat
    21061 gttaccagct gaggttgtca acaatacgtg cgggtcccct ttcgctgcca ttgctgcggg
    21121 agaaagagca gaaagagcga aaacagcagc catcgccgcc agggtcgatt tgaacatgag
    21181 atttccttaa caaacagagc agaaaaaaag caagtgaaga gattctaaat agccttcaag
    21241 aacaaggcca tccctttacc taattttacg tatctgaaat atatgtaacc tgacgatcac
    21301 tttttattcc gtgatcaaaa tcacctctta aaatgcaatt tagcaaccga ttgcaataaa
    21361 acatttaaac agatcacaaa atcacctaaa atcgcccgct cgcggcatat cccgctatgc
    21421 cattttattt ttgaacgcag gccaatcatg actaacagca atcgcatcaa gctcacatgg
    21481 attagctttc tctcctacgc actgactggt gcgttggtta ttgtcaccgg gatggtgatg
    21541 ggaaatatcg ccgattattt caatctgcct gtttccagta tgagtaacac cttcaccttc
    21601 ctcaacgccg gcattttaat ctctatcttc ctcaacgcct ggctgatgga aatcgtcccg
    21661 ttgaaaacgc agttacgttt tggctttctc ctgatggtgc tggcggttgc cggtttgatg
    21721 ttcagccaca gcctggcgct gttctcggcg gcgatgttca ttctcggggt ggtcagcggc
    21781 atcaccatgt cgattggtac attcctggta acacaaatgt atgaagggcg tcagcgcggt
    21841 tcccgcctgt tatttaccga ctccttcttc agtatggctg ggatgatttt cccaatgatc
    21901 gccgcgtttc tactggcgcg cagcattgag tggtactggg tttatgcctg catcgggctg
    21961 gtgtatgtcg ctatttttat tctgaccttc ggctgtgagt tcccggcgct gggcaaacat
    22021 gcaccaaaaa cggatgctcc ggtagcgaaa gaaaagtggg ggatcggcgt actgtttctc
    22081 tctgttgcgg cgctgtgcta catcctcggt cagttaggtt ttatctcctg ggtgcctgag
    22141 tatgccaaag gcctgggcat gagcctgaat gatgctggca cgctggtgag taacttctgg
    22201 atgtcataca tggtcggcat gtgggcgttc agctttattc ttcgcttctt tgatttgcaa
    22261 cgcattctga ccgtactggc tggtctggct gcgattctga tgtacgtctt taacaccgga
    22321 acaccggcac atatggcgtg gtcaattctc gccctgggct tcttctccag cgcgatctat
    22381 accaccatca tcaccctggg ttcacagcag accaaagtac cgtcgccaaa actggttaac
    22441 tttgtcctga cctgcggaac catcggtact atgttgacct ttgtggttac cggcccgatt
    22501 gttgaacata gcggtccgca ggcggcactg cttacagcaa acggtctgta cgctgtcgtc
    22561 tttgtgatgt gcttcctgtt aggtttcgtc agccgtcacc gtcagcataa caccctgacc
    22621 tctcattaat tgctcatgcc ggacggcact atcgtcgtcc ggccttttcc tctctaaccc
    22681 cgctacgtgc atctatttct ataaacccgt tcattttgtc tattttttgc acaaacatga
    22741 aatgtcagac aattccgtga cttaagaaaa tttatacaaa tcagcaatat accccttaag
    22801 gagtatataa aggtgaattt gatttacatc aataagcggg gttgctgaat cgttaaggta
    22861 ggcggtaata gaaaagaaat cgaggcaaaa atgagcaaag tcagactcgc aattatcggt
    22921 aacggtatgg tcggccatcg ctttatcgaa gatcttcttg ataaatctga tgcggccaac
    22981 tttgatatta ccgttttctg tgaagaaccg cgcatcgctt atgaccgcgt acacctctcg
    23041 tcttacttct ctcaccacac cgccgaagag ctgtcgctgg tgcgcgaagg cttctacgag
    23101 aaacacggca tcaaagttct ggtcggcgaa cgcgctatca ccatcaaccg tcaggagaag
    23161 gtgattcact ccagcgccgg acgtaccgtt ttttatgaca agctgatcat ggcgaccggt
    23221 tcctacccgt ggatcccgcc aattaaaggt tctgatactc aggactgctt tgtctatcgc
    23281 accattgaag acctcaacgc cattgaatcc tgcgcccgtc gcagcaaacg cggtgccgtt
    23341 gttggtggcg gcctgttagg tctggaagcc gctggcgcgc tgaaaaactt aggtattgaa
    23401 acccacgtta tcgaatttgc ccctatgctg atggcagaac agcttgatca gatgggtggc
    23461 gagcagctgc gtcgcaaaat cgagagcatg ggcgtgcgcg ttcacaccag caaaaacacc
    23521 cttgagattg tgcaggaagg tgttgaagcg cgtaaaacca tgcgttttgc cgacggcagc
    23581 gaactggaag tcgactttat cgtcttctct accggtatcc gtccgcgcga taagctggca
    23641 acccagtgtg gtctggacgt tgctccgcgt gggggtattg tcattaatga ttcctgccag
    23701 acttccgatc cggatatcta cgccatcggt gaatgcgcaa gctggaacaa ccgtgtattt
    23761 ggtctggtag caccaggcta caaaatggcg caggtcgccg ttgaccatat tctcggtagc
    23821 gaaaacgcct ttgaaggtgc tgaccttagc gccaagctga aactgctggg cgtagacgta
    23881 ggcggtattg gtgatgcgca cggtcgcacg cctggcgcac gtagctacgt ttacctcgac
    23941 gaaagcaaag agatctacaa acgccttatt gtcagcgaag acaacaaaac cctgctcggt
    24001 gcggtactgg tgggcgatac cagcgactac ggcaacctgc tgcaactggt gctgaatgct
    24061 atcgagctgc cggaaaaccc ggattctctg attctgccgg cgcactcggg tagcggtaag
    24121 ccgtctatcg gtgttgataa actgccggac agcgcgcaaa tctgctcctg cttcgacgtc
    24181 accaaaggtg atctgattgc tgccatcaac aaaggctgtc acaccgttgc ggcgctgaaa
    24241 gccgaaacca aagcgggtac tggctgcggt ggctgtatcc cgctggtcac tcaggtactg
    24301 aacgcggaac tggcgaaaca gggcatcgaa gttaacaaca acctgtgcga acactttgct
    24361 tattcgcgtc aggaactatt ccatttgatc cgcgttgaag gcattaaaac cttcgaagaa
    24421 ctgctggcga aacacggcaa aggttacggt tgtgaagttt gtaaaccgac cgtcggttcg
    24481 ctgctggcct cctgctggaa cgaatacatt ctgaagccgg aacatactcc gctgcaggat
    24541 tctaacgaca acttcctcgc taacatccag aaagacggca cctactcggt gatcccgcgc
    24601 tctccgggcg gtgaaatcac cccggaaggg ctgatggcgg taggtcgtat cgcgcgtgaa
    24661 tttaatctct acaccaagat cactggctcc cagcgtctgg cgatgtttgg cgcacagaaa
    24721 gacgatctgc cggagatctg gcgtcagctg attgaagccg gcttcgaaac cggtcatgcc
    24781 tatgcgaaag cactgcgtat ggcgaaaacc tgcgtgggta gcacctggtg ccgctacggc
    24841 gttggcgaca gcgtcggcct cggcgtggaa ctggaaaacc gctacaaagg catccgtacg
    24901 ccgcacaaaa tgaagttcgg tgtctccggc tgtacccgtg aatgttcaga agctcagggt
    24961 aaagacgtgg gtattatcgc cactgaaaaa ggctggaacc tgtatgtttg cggtaacggc
    25021 ggcatgaaac cgcgtcatgc ggatctgctg gcggcggata tcgatcgcga aacgctgatc
    25081 aaatatctcg accgcttcat gatgttctac atccgtactg ccgacaaact gacgcgtacc
    25141 gcaccgtggt tagaaaacct cgaaggcggc atcgattacc tgaaagcggt gatcattgac
    25201 gacaaactgg ggctgaacgc acatctggaa gaagagatgg cgcgcctgcg tgaagcggta
    25261 gtgtgtgagt ggactgaaac ggtcaatacg ccgtctgcgc agactcgctt caaacacttc
    25321 atcaacagcg acaagcgtga cccgaacgtg cagatggtgc cagagcgcga acagcaccgt
    25381 ccggcaacgc cgtatgaacg tatcccagta actctggtgg aggacaacgc atgagccagt
    25441 ggaaagacat ctgcaaaatc gatgacatcc tgcctgaaac cggcgtctgc gcgctgttag
    25501 gtgacgagca ggtcgcgatt ttccgcccgt atcacagcga tcaggtgttt gcgatcagca
    25561 acatcgaccc gttcttcgag tccagcgtgc tgtcacgcgg actgattgcg gaacaccagg
    25621 gcgagctgtg ggtcgccagc ccgctgaaaa aacagcgttt tcgcttaagc gacggcttgt
    25681 gcatggaaga cgaacagttt tccgtcaaac attacgaagc gcgagtgaaa gacggcgtgg
    25741 tgcagctgcg cggttaatgt tttaacggga ggcgcaatgc ctcccctttt tgcatggtcc
    25801 tgtaataacc ttcggtatat tgcaggacat tttttaaact ttttgtttta ttttttgttt
    25861 ttatttttta aaggataatc aaatgtttac agacactatt aataagtgtg cggctaacgc
    25921 tgcgcgcatt gcacgcctgt cggcaaataa cccgctcggc ttttgggtca gctccgccat
    25981 ggcgggcgcg tatgtgggtc ttgggatcat cctgattttt acgctcggta atttgctcga
    26041 tccatccgta cgccctctgg tgatgggcgc gacctttggt atcgccttaa cgctggtgat
    26101 tatcgccggt tctgagctgt tcaccggaca caccatgttc ctcacctttg gggtgaaagc
    26161 gggcaccatc agccacgggc aaatgtgggc aatcctgccg caaacctggc tgggtaacct
    26221 ggtcggttcc gtcttcgttg ccatgctcta tagctggggt ggtggtagcc tgctgccggt
    26281 agataccagc atcgttcact ccgtcgcgct ggctaaaacc actgcaccgg caatggtact
    26341 gttcttcaaa ggtgcattgt gtaactggct ggtttgtctg gcaatctgga tggcgctgcg
    26401 cactgaaggg gcggcgaaat ttatcgcaat ctggtggtgt ctgctggcat ttatcgcgtc
    26461 cggctacgag cactccatcg ctaacatgac gctgttcgcg ctctcctggt tcggcaacca
    26521 cagcgaagcc tacacgctgg cgggtattgg tcataacctg ctgtgggtga cgctgggtaa
    26581 tactttatca ggtgccgtat tcatgggatt gggttattgg tatgctacgc cgaaagcgaa
    26641 tcgtccggtt gcggacaaat ttaatcaaac tgaaacggct gccggttaat tactaagggg
    26701 tttttacgtg gatcatttgc ctatattttg ccaattacgc gatcgcgact gtctgattgt
    26761 cggcggtggt gatgtcgcgg aacgcaaagc aaggttgctg ttagacgcag gcgctcgctt
    26821 aacggtgaat gcattagcgt ttattccaca gttcaccgca tgggcagatg caggcatgtt
    26881 aaccctcgtc gaagggccat ttgatgaaag ccttctcgac acctgctggc tggcgattgc
    26941 agcgacggat gatgacgcgc ttaaccagcg cgtcagcgaa gccgctgaag ctcgtcgcat
    27001 cttctgtaac gtggtcgatg cgccgaaagc tgccagcttc attatgccgt cgattattga
    27061 ccgctcaccg ctgatggtgg cggtttcctc tggcggtact tctccggttc tggcgcgcct
    27121 gttgcgcgaa aaactcgaat cactgctgcc gctgcatctg ggccaggtag cgaaatacgc
    27181 cgggcagtta cgcggtcggg taaaacaaca gttcgccacg atgggtgaac gccgccgctt
    27241 ctgggaaaaa ctgttcgtta acgaccgtct ggcgcagtcg ctggcgaaca acgatcagaa
    27301 agccattact gaaaccaccg aacagttaat caacgaaccg ctcgaccatc gcggtgaagt
    27361 ggtgctggtt ggtgcaggtc cgggcgatgc cgggctgctg acactgaaag gtctgcaaca
    27421 aattcagcag gcagatgtgg tggtctacga ccgtctggtt tctgacgata ttatgaatct
    27481 ggtacgccgc gatgcggatc gtgttttcgt cggcaaacgc gcgggatacc actgcgtacc
    27541 gcaggaggag attaaccaga tcctgctgcg ggaagcgcaa aaaggcaaac gcgtggtgcg
    27601 gctgaaaggc ggcgatccgt ttatttttgg ccgtggtggc gaagagctgg aaacactgtg
    27661 caacgcgggt attccgttct cggtggttcc gggtattacc gcagcttctg gttgctcagc
    27721 ctattcgggt attccgctca cgcatcgcga ttatgcccag agcgtgcgct taattaccgg
    27781 acacttaaaa accggtggcg agctggactg ggaaaacctg gcggcagaaa aacagacgct
    27841 ggtgttctat atggggctga atcaggctgc gactattcag caaaagctga ttgaacacgg
    27901 tatgcctggc gaaatgccgg tagcaattgt cgaaaacgga acagcagtca cgcagcgcgt
    27961 gattgacggt acgctcacac aactgggcga actggcgcag caaatgaata gtccatcgct
    28021 aattattatt ggtcgggttg ttggcctgcg cgataaactg aactggttct ccaaccatta
    28081 atttaatcag ggccagagaa ttctggcctt tttcgtttac aactctcttt atatattcag
    28141 cagataaaaa tataactcat taacaaaaat catttgtttt tgccccgtta tattcaccgt
    28201 tacagcgact tacaaaatta agtcattcac tcactgacgc cgattaaata ttcctgtatc
    28261 gttcttaatg agcagtcta
//
LOCUS       DAZFAB010000052        27833 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZFAB010000052 DAZFAB010000000
VERSION     DAZFAB010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27833
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.052"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            87..749
                     /gene="acrS"
                     /locus_tag="ACHJWA_002617"
     CDS             87..749
                     /gene="acrS"
                     /locus_tag="ACHJWA_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFO4309192.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            complement(752..931)
                     /locus_tag="ACHJWA_002618"
     CDS             complement(752..931)
                     /locus_tag="ACHJWA_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFO4309193.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(1015..1899)
                     /gene="yhdJ"
                     /locus_tag="ACHJWA_002619"
     CDS             complement(1015..1899)
                     /gene="yhdJ"
                     /locus_tag="ACHJWA_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO4309194.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLLEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLITK"
     gene            complement(1984..2280)
                     /gene="fis"
                     /locus_tag="ACHJWA_002620"
     CDS             complement(1984..2280)
                     /gene="fis"
                     /locus_tag="ACHJWA_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFO4309195.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(2306..3271)
                     /gene="dusB"
                     /locus_tag="ACHJWA_002621"
     CDS             complement(2306..3271)
                     /gene="dusB"
                     /locus_tag="ACHJWA_002621"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFO4309196.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(3600..4481)
                     /gene="prmA"
                     /locus_tag="ACHJWA_002622"
     CDS             complement(3600..4481)
                     /gene="prmA"
                     /locus_tag="ACHJWA_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFO4309197.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(4493..5944)
                     /gene="panF"
                     /locus_tag="ACHJWA_002623"
     CDS             complement(4493..5944)
                     /gene="panF"
                     /locus_tag="ACHJWA_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFO4309198.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5934..6176)
                     /locus_tag="ACHJWA_002624"
     CDS             complement(5934..6176)
                     /locus_tag="ACHJWA_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFO4309199.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(6285..7634)
                     /gene="accC"
                     /locus_tag="ACHJWA_002625"
     CDS             complement(6285..7634)
                     /gene="accC"
                     /locus_tag="ACHJWA_002625"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFO4309200.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNSIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(7645..8115)
                     /gene="accB"
                     /locus_tag="ACHJWA_002626"
     CDS             complement(7645..8115)
                     /gene="accB"
                     /locus_tag="ACHJWA_002626"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFO4309201.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(9093..10067)
                     /locus_tag="ACHJWA_002627"
     CDS             complement(9093..10067)
                     /locus_tag="ACHJWA_002627"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFO4309202.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            10219..12159
                     /gene="csrD"
                     /locus_tag="ACHJWA_002628"
     CDS             10219..12159
                     /gene="csrD"
                     /locus_tag="ACHJWA_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFO4309203.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            12464..13507
                     /gene="mreB"
                     /locus_tag="ACHJWA_002629"
     CDS             12464..13507
                     /gene="mreB"
                     /locus_tag="ACHJWA_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFO4309204.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            13573..14676
                     /gene="mreC"
                     /locus_tag="ACHJWA_002630"
     CDS             13573..14676
                     /gene="mreC"
                     /locus_tag="ACHJWA_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFO4309205.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSLQRATPPQSGAQPPARAPGGQ"
     gene            14676..15164
                     /gene="mreD"
                     /locus_tag="ACHJWA_002631"
     CDS             14676..15164
                     /gene="mreD"
                     /locus_tag="ACHJWA_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFO4309206.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            15173..15766
                     /gene="yhdE"
                     /locus_tag="ACHJWA_002632"
     CDS             15173..15766
                     /gene="yhdE"
                     /locus_tag="ACHJWA_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFO4309207.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKARAGVAQTAKDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSRHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYQAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15756..17225
                     /gene="rng"
                     /locus_tag="ACHJWA_002633"
     CDS             15756..17225
                     /gene="rng"
                     /locus_tag="ACHJWA_002633"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFO4309208.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            17293..21093
                     /gene="yhdP"
                     /locus_tag="ACHJWA_002634"
     CDS             17293..21093
                     /gene="yhdP"
                     /locus_tag="ACHJWA_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFO4309209.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKET
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLGIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPAAVNEALSGSVAWDGKVGIVLPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLPVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTQVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWSDAQIRCAECWFWGQKFGRIDSDITISGNTLTLTNGLI
                     DTGFSRLTADGEWINNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            21249..22694
                     /gene="tldD"
                     /locus_tag="ACHJWA_002635"
     CDS             21249..22694
                     /gene="tldD"
                     /locus_tag="ACHJWA_002635"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFO4309210.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFVILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIARD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLIEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22828..23757)
                     /gene="aaeR"
                     /locus_tag="ACHJWA_002636"
     CDS             complement(22828..23757)
                     /gene="aaeR"
                     /locus_tag="ACHJWA_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFO4309211.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23940..24143
                     /gene="aaeX"
                     /locus_tag="ACHJWA_002637"
     CDS             23940..24143
                     /gene="aaeX"
                     /locus_tag="ACHJWA_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFO4309212.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVCRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            24151..25080
                     /gene="aaeA"
                     /locus_tag="ACHJWA_002638"
     CDS             24151..25080
                     /gene="aaeA"
                     /locus_tag="ACHJWA_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFO4309213.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHNQLVKKGQVLFTIDQPRYQKALEEAQADVAYYQVLAQE
                     KRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAPA
                     DGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGSN
                     KVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWPA
                     GTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            25086..27053
                     /gene="aaeB"
                     /locus_tag="ACHJWA_002639"
     CDS             25086..27053
                     /gene="aaeB"
                     /locus_tag="ACHJWA_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFO4309214.1"
                     /translation="MDIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LCIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            27145..27417
                     /locus_tag="ACHJWA_002640"
     CDS             27145..27417
                     /locus_tag="ACHJWA_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFO4309215.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(27473..27736)
                     /gene="yhcN"
                     /locus_tag="ACHJWA_002641"
     CDS             complement(27473..27736)
                     /gene="yhcN"
                     /locus_tag="ACHJWA_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFO4309216.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         6679 a         6959 c         7414 g         6781 t
ORIGIN      
        1 tcgtataaat acacattagg tgatagatta accttcgcta ttttctcact ctgtgtcgaa
       61 gatatttatt tcctgaataa ttaatcatgg caaaaagaac caaagccgaa gctctgaaga
      121 cccggcaaga actgattgaa actgccatcg cccagtttgc gcaacatggc gtaagcaaga
      181 cgacgctcaa cgacattgcc gatgccgcta acgttacgcg tggcgctatc tactggcact
      241 tcgagaacaa gacccaactg tttaatgaga tgtggttgca acagccttca ttgcgggagt
      301 taatccagga acacttgacg gctggattag agcatgaccc gtttcaacaa ttgcgtgaaa
      361 aattgattgt cggcttgcaa tatattgcca aaattccccg ccagcaggcg ttgctgaaaa
      421 tcttatatca caaatgtgaa tttaatgatg agatgctggc cgagggagtg atacgcgaaa
      481 agatgggctt taatccgcag actctccgcg aagtattgca ggcgtgtcag caacaaggtt
      541 gtgtagcaaa taacctcgat ttagatgttg tgatgattat tattgatggt gccttcagcg
      601 gaattgttca aaactggtta atgaatatgg cgggttatga tctttataaa caagcccccg
      661 ctctggtcga taacgtatta agaatgttca tgccagatga aaacataacg aaattaattc
      721 atcaaacgaa tgaattaagt gtcatgtaaa ttcacattct gtcctgaaaa ttcagttcgt
      781 cacagttaac cagttttagt ggattaactg agttcatatt gcggcatttg tctgtttctg
      841 ttgccagcag ttcaatccac tgcatcagta aagtatcaaa cagaaaaacg aagacagcga
      901 aaacgacgag ccaccaatac ttgcgaatca ttacctgagc ctgtgcatgt ttaaccaagt
      961 ctggcaggaa atatacacga aatgaataat ctgcaaacca ggcttacaca tcctttactt
     1021 tgtaatgaga tcggggtcaa cttcgcttaa ccggcttcgt tttgacaggt tgccggtctt
     1081 tctttttttc actttcgcca gttcttccgc agagtaatgc gacgcgacat ccagccgtcg
     1141 aagccccatt ttgatgtact cgctgttgat ctcaatacca atgaattttc gtccgctggc
     1201 gatggctacg gcaccggtag taaagctacc agcaaacggg tcgagaacga tatcgcctgg
     1261 gttggaagag gcgagaataa tgcgtttcag taaggcttcc ggtttttgcg tcgggtggtt
     1321 ttcatattca tccatcaaat aacgcacgcg cggaaaatcc cagacgttac ccggtacttt
     1381 ttgatgattg taaggctgtg gaggattttt gcgataatcg atcaacgcgc gctgcgatcc
     1441 ggttttggct tctaccagaa tagcatcacc gttgaatgtg tagttctttg cgtctttcac
     1501 catcatcagg atgggttcgt acatggagcc gtagtgtttt ttcgcctgca ctccagaact
     1561 gtcatatgac cagacgatgc gacttttgat ggtaaaaagc ttgcggcact ggagatcgat
     1621 aaagggcatg ttttccgtac tgttcatgat gtacatgctg ccctgctttt tcagaacgcg
     1681 gtggcactct gcaatcactt ctaacagcca gtcgataaac agatcttctt tccaggcttc
     1741 gatcagacca tcaaaatttt taccgatgtt atacggtggg tcggcaaaga tcagatcgac
     1801 gctttcagta ggtagctttt taagttcggc aagggcatca ccgtgaataa tggtcttagc
     1861 ttcattacca aaccgggtcg gttcacatcc tgttctcatg gtcactccct ttgtgacacc
     1921 tataaaaaag gcgcttcccc atgccgagta gcgcctttta atcaagcaat tagctaacct
     1981 gaattagttc atgccgtatt ttttcaattt tttacgcagc gtaccacggt tgatgcccat
     2041 catcagcgca gcacgggtct ggttaccacg ggtgtattgc atcaccatgt ccaacagggg
     2101 ctgttctact tcagccagta ccagctcata gaggtcattc acatcctgac cattcagttg
     2161 agcaaaatag ttcttcagtg cctgtttaac cgagtcacgc aggggttttt gggttacctg
     2221 atcctgagag ttaacggtag aaacggtcag tacgtcagaa tttacgcgtt gttcgaacat
     2281 agttctgtca gctctttatt tctgtttacg caaaattttc gaagtatgcc tccaacgcct
     2341 ccagctgttc gctggcatcc tcaatggcgt tgaatgtgcg ccgaaactgg tcatttggag
     2401 cgtgttcctg gagataccag gaaacgtgtt tacgtgcaat tcggtaccct tttgccggac
     2461 cataaaagtc atgcagttcc cgaacgtgcg cgcaaagcaa gcgcttaacc tctgccaaag
     2521 gcagcggggg cagcaactcc ccagtgtcca gataatgctg gatttcccga aagatccagg
     2581 gtcttccctg agctgcgcgg cctatcatca gggcatccgc ccctgtatag tcgagcacag
     2641 ctctggcttt aagcgggtca gtaatgtcgc cattcgcgat aaccggaatg gaaactttct
     2701 gcttaactgc ccgaatactg tcgtactcag cttctccatt gaacaaacag gcgcgtgtac
     2761 ggccatgaat ggtcagagcc tgaatgccac agtcttcagc cagttgggca atctcttcgc
     2821 agttacggtg ttccggtgcc cagccggtgc gaatcttcag ggtaacagga acgtccactg
     2881 cattgacgac ctcggtaagg atcgatttaa cgacatccgg gtactgcaag agggctgaac
     2941 ctgcgagctt gcgattcact tttttagccg ggcaacccat attgatatca ataatctggg
     3001 caccgctttc cacgttaata cgtgctgcat ctgccatttc tttcggatcg ctaccagcaa
     3061 tttgcacggt gcgaataccg ggttcatcaa tgtgcaccat ccgtaaacga gatttgtcgc
     3121 tttcccaaac ctgtgggtta gaagacatca tctcggatac tgtcaatccg gctcccatct
     3181 cgtagcacaa cgtccgaaaa ggtctgtctg taatgccagc catgggcgct gcgatcaggc
     3241 gatttctgag ctgatattgt ccgatgcgca tgagttaaga aatgaccata ctgtgactgc
     3301 aaggcggcgt atattacgca ttttttgcac gagatgaaag gccaaacttt gaccaatcct
     3361 ctgagatgga tcaaagaatt gcatttaaaa tgagcgtggt gcgataatta ctcataaaaa
     3421 tcatcatatt agaaaatagt gactaaaatt tacactcaaa gaaatttgag taagttctca
     3481 atttttcttt atgaatgaaa attttggcac gcaaatttgc gtaaataatc ggcaaattta
     3541 cgtgcctttt gtgagcttgc tcgcacttcg ccccgcgtca ccctatggcg atgcgaaggt
     3601 taattcttac gaccggtaat acggcaccac tcttcttttt ccacgaccgg gtccagagcg
     3661 aagctgtcgg cataggcttc gcaaacgctc tccgcctggc ttgccagaat accagaaagg
     3721 cccagtaagc cgcctgaaac tggcaggacg ctaattaacg gtgccagttc acgtaatggg
     3781 cctgcaagga tgttagcgac caccacgtcg gctttcattt cttctggctg atctttcggt
     3841 aagtagagtt ccagacggtc agaaacgcca ttacgttcgg cgttatcgcg gctggcctga
     3901 atcgcctgcg gatcgatatc aataccaatg gcttttgctg cacccagttt cagcgccgcg
     3961 atcgccagaa tgccggaacc acagccaaag tcgatgactg ttttaccggt taaatcgagg
     4021 ctgtcgagcc attgcaggca cagagaggtg gttggatggg tacccgtacc aaacgccagc
     4081 cctggatcta acatcacgtt gacggcgttt tcgtccggca catcacgcca gctagggcag
     4141 atccacagtc gttcgccaaa gcgcatcggg tggaaattat ccatccattc gcgctcccag
     4201 tctttatctt ctagttgttc gattttatgc gcgaagcctg cgccgagcag cggatggttt
     4261 tccagaatcg ccaccacgtc gttcatatcg gtttcagcgt cgaacagacc aatcacatcg
     4321 gtgtcgcccc acaggcgcgt ttcgcccggc agcggctcaa atactggcgt atcgtgggta
     4381 tcctgaaaag tgatagaaac ggcacccgct tccatcagcg catcgctaag atcttccgcg
     4441 ttcgcgccgg tggtgttcag tttcagttgg atccaaggca tggcaaaact ctttatttat
     4501 cagtagtcaa aacggtagct tgcgggacgg atgtaccgaa acggtttccg accaggaaag
     4561 ccagcaaact tagtagtaac gagggcacga tagggtggaa gcccaggtac tgaatattca
     4621 gcgtcgcgag tacggcatac agcacgccgc caacgatcat cgcacttagc gcgcctttgg
     4681 cgttggcgcg ttcccagtaa agacccagca ccagcggcca caggaaaacg gcttccagcc
     4741 caccgaaggc cagcaaattc agccagatga tcatttctgg cggcttccag gcggcaagca
     4801 gcagcaacgc gccgagaact aacgtaatta ccgccgacat ccgcttcaga cgcgtctcgt
     4861 tttgcatttg atccggacgg atattcagat agagatcttt aatgatcgta gcggaacttt
     4921 gtagcaattg ggcgttaatt gtcgacatga tcgcagccat aggtgcagcc agaaagatcc
     4981 cggcagcaaa cggtggcagc acttttacca ttaacgtcgg gatcaccaga tccggtacgg
     5041 tgagatcggg gatcactgca cgacctaacg ctccggctag gtgcataccg aacatcagga
     5101 ttgcgactac aatcgtaccg atgatgatcc cccggtgtac tgctttgctg tctttataag
     5161 agatacagcg caccgcagta tgcggcaggc ctataacgcc aaagcacacc agtacccaga
     5221 acgacgtcat aaaggcaggc gacagaatat cgtcagcgcc ttgtggcgta accagttgcg
     5281 gatcgatggt ttgcaaggtc tgtactgcgt tgcttaagcc gccagcggca tgtaccacgc
     5341 caataagcag cacaacggtg ccaatcagca tcacaagccc ttgcatggtg tcgttcagca
     5401 cgctggcgcg aaagccacca aaggcggtat ataacgcaat gctgatacca aaaatcagca
     5461 gcccggtttc ataaggaata cccgccgcgg tttccagcag gcgcgcaccg ccgataaact
     5521 gcacggtcat cgcaccaacg aacgcaacca gcaaactcaa actcgccagc cacaccagaa
     5581 gacgactctg gtagcgggca aacagcatat cgttcagcgt cactgcattg tagcggcgcg
     5641 caagaatcgc aaacttcttg ccgagaatac cgagtgaaag ccagactgcc ggaagctgaa
     5701 tcatcgccag caatacccag ccaaggccgt atttgtaagc agctccaggc ccgccgataa
     5761 acgaactggc actgatataa gtcgcggtga gcgtcatcgc cagcacaata ccgcccatag
     5821 agcggctgcc gaggaaatac tcattaagga aggtgccggt gctccgttta cgcatcgcat
     5881 aaaccgagat accgaacacc accaccagat aggcgaccag cggtagaatt acttcaagct
     5941 gcatcgtcat cctccagcgg gatatcgcga tagataaatt tcaccatcgc ccagcacagt
     6001 ccaataaaca gcagcggcgt caggatgcag gccatctcaa accagcgcgg aaagccggta
     6061 aaaccggggg caacgccagg taagtaagcg gctactaacc aaactgccag atacaaaagg
     6121 gtcagcccca gcgcccagcg cgcctcttta tgggcctgaa caaaacgagt gtccattttt
     6181 tgtccctgat gggtgaagaa agcgaggatt gtaccttatg ggtgttgtcg atccccagta
     6241 ataaaaaagg ccggaaaatc cggccttttg acgcttttgc agtcttattt ttcctgaaga
     6301 ccgagttttt tctccagata gtggatgtta gtgccaccat gctggaagtt ctcgtcattc
     6361 atgatgcgga tctgcagatc aacgttggtt ttgataccgt cgatgatcag ctcctgcagc
     6421 gcattcttca tgcgggcaat cgccacgtca cggttttcac cgtagcaaat cagcttaccg
     6481 atcattgagt catagtacgg cggtacggtg tagcccgcgt agatatgaga ctcccaacgt
     6541 acgccaaaac cgccaggtgc gtggaaacgg gtgattttgc ccggacttgg caggaaggtg
     6601 ttcggatctt cggcgttgat acgacattcc accgcatggc cgcgaacgtg aacttcttct
     6661 tgcttgatcg acagcggttg accggcagcg atacgcagct gttctttgat caggtcaacg
     6721 ccggtgatca tttctgtaac cgggtgttct acctgaatac gggtgttcat ttcgatgaaa
     6781 tagaactcgc cgttttcgaa caggaactcg aaagtacctg caccgcgata gccgatatca
     6841 acacacgctt tagcgcaacg ttcgccgatg tagcgacgca gttccggggt aatgcccggt
     6901 gctggtgctt cttcgaccac tttctggtgg cggcgctgca tggagcagtc acgttccgcc
     6961 agatagatag agttgccctg accgtcagcc agtacctgaa tctcgacgtg gcgaggattt
     7021 tccaggtatt tctccatgta aaccatatcg ttgctgaaag cagctttcgc ttccgcacgg
     7081 gtcatggaga tggattgtgc cagttcagcg tcgccgcgca ctacgcgcat accgcgacca
     7141 ccgccgccgc cggaggcttt gataatcacc ggataaccaa tgcgtttagc aatggcacgg
     7201 tttttatcca tatcgtcgcc cagcgggccg tcagaacccg gtacgcaagg gacgcccgct
     7261 tttttcatcg ccgcgattgc ggatactttg tcgcccatca ggcgaatggt ttctgctttc
     7321 gggccaatga agataaagcc ggagcgttca acctgctcgg caaagttggc gttctcggag
     7381 aggaagccgt aacccggatg gattgctact gcgccggtga tttcagcggc gctgatgatt
     7441 gccgggatgt tcagataact ttttactgac ggagcagggc caatacagac cgtttcatct
     7501 gccagtaata cgtgttttag atcgcgatcc gcgctggagt gcacagcgac agtcttgatg
     7561 cccagttctt tacaggcacg aagaatacgc aatgcaatct cgccgcggtt ggcaataaca
     7621 attttatcca gcatgttcgc ctcgttactc gatgacgacc agcggctcgt caaattctac
     7681 cggttgtcca ctttcgacca gaattgcttt cacggtaccg gatttgtccg cttcgatctg
     7741 gttcatcatt ttcatggctt caacgatgca cagggtatcg cccacgttga ctttctgacc
     7801 cacttcgatg aacgcttttg catccgggct tggggtgcgg tagaaagtac caaccatcgg
     7861 ggaacgtacg atgtgaccac tgatttccgc tgctgctggc gcttccatgg aaggaacggt
     7921 cgccggagcg gctgcgttag attgagctgg ctgctgcatc attggtgcag cgtaagcttg
     7981 ttgcatcaca gggaaacttg cggcaggagc tgcacggcta atgcgtactg actcttcgcc
     8041 ttcagaaatt tccagttcgg agatgcctga ttcttcaacc agctcgatca gttttttaat
     8101 cttacgaata tccatgagtg ggttccgtac tctttgttta gtgtgattgt gacaggcgtt
     8161 tcaccgccgt ctgtaaagcg tatgccccga cagctgtatg catagcgata aattccagca
     8221 ggtcgggacg ccggtctatt ttgccccaga gtgtcaatgt tagacttgac ggacattgtg
     8281 cagcgatctg ctgctatcct ccggcaaaaa acaaaatata ccttcgttgc gccaatgtca
     8341 ccttttccgc agcgcaaaaa ctgcgtcagg gaggacgagg tcgcacatta taacgatttc
     8401 gtagcaattg gcagctaaat actggtctta tcagggaaga taatcaacaa ctaacatgta
     8461 aataaccttc aacaccgtgt aatttgcaac aagccgcaca attcacgaaa ttagcgccat
     8521 catcgacgga acttcttata acgtaaggtg aaaagctgta aagccagccc tgcgaagatg
     8581 aggggctgca atgagataat cgtcacagac cacaaataat gtagggatgc caggatcgcg
     8641 acaagataga cgacattacg cagcggttgc tggggattac ccaattttca ctgcatcgcc
     8701 tggatagatg tgaatgctaa agcacgcaaa attaaccgat tgacaatgct cagcattaaa
     8761 taggttcgag tgattaattt gtagtcaaac aaaatcagat tattcacctc atggtaccag
     8821 aaataagtaa ggggttaagc gtagcgtcgc ccaggcaaag caccataaac ctaacaggcg
     8881 acggaggcgt atcaataata gctgtttagt ggagtatcaa tgcgcatcaa tttatggtca
     8941 gtcgaaatca tgactttcca gtttggatga tgttgtacaa cttcatgacc aaggtgttcg
     9001 acgaaagctg tccggctaac cgcttttccc gttaaccgat tgaataatga tttcaaaatg
     9061 agttccctct cttattattc cttgctaaat ggttagttaa ccttcaccag cgtgcgaccc
     9121 tggatctggt tattaatgat ggcctcggca aagttcggtg cctctgacag agatatctct
     9181 tttgccgcct gggtatagaa tgattccggt aaatcggcga ccagtcgctg ccaggcttgt
     9241 gcgcggcgtt ctggtggcgt cattactgaa tccacccctt gcaaacggac attacgcaga
     9301 ataaatggca tgaccgtggt tggcagagta aaaccacccg ccagaccaca ggccgccacg
     9361 cagccgccgt aattcatttg cgccagcact tttgccagca ctttgtcgcc aacagtgtca
     9421 attgccccag cccagacttg tttttccaga ggacgggatt cggcaaactc atcacgaggg
     9481 agaatacggc tggcacctaa acttttcaga tattcatggg tactttcgcg accggaaacg
     9541 gcaacgacct gataacccaa cttatgcagc agcgccacgg cggtactgcc gacgccacca
     9601 ctggcacccg tcacgacaat ctccccgtcc tgcgggcgaa caccggcatc ttccagcgcc
     9661 atcacacaca gcatggcggt aaaaccggca gtaccgataa tcattgcttt acgcgcgtcc
     9721 agcccttgcg gcatggcaac cagccagtca cctttcactc gcgcctgctc cgccagccca
     9781 ccccagtggt tttcaccaac gccccagcca gtgagtaaca cctcctgacc ggcatgaaaa
     9841 cgcggatctt cgctggtgcg tacagttccg gcaaaatcga tcccaggaat catcggaaaa
     9901 ttacggatga tttttccctt accggtaatc gccagcgcat ctttatagtt caggctcgac
     9961 cagtgaacat cgaccgtgac atcgccctcc ggcaggcgac tttcgtccag agtctgtact
    10021 gatgcgagag ttttgccgtc ctgctgttct aaaagtaacg cctgcataag tggtcctcat
    10081 gtgcatgatg gattggaaaa taatttctga agactatact cgctaatgga aaagcaaagc
    10141 cgatgaagcg caataaattg cgagataaat ctgatttgct agtatgcccg cttcctcact
    10201 atcggagtta acacaaggat gagattaacg acgaaatttt cggcctttgt tacgctgctc
    10261 accgggttaa caatttttgt gactttgctg ggctgttcgc taagtttcta caacgccatt
    10321 cagtataagt ttagtcatcg cgttcaggcg gtggcgacgg cgattgatac ccaccttgtg
    10381 tcgaatgact tcagcgcatt aaggccacaa attaccgaat taatgatgtc ggcagatatc
    10441 gttcgtgtag acctgctcca tggtgataag caggtttata ccctggccag aaatggtagt
    10501 tatcgtccgg ttggcaccag cgatctgttt cgtgaactga gcgttccgtt gataaagcat
    10561 ccggggatgt cgttgcgtct ggtttatcag gatccgatgg gcaactattt ccattcgttg
    10621 atgaccaccg cgccgctcac aggggcgatt ggctttatca ttcttatgct ctttctggcg
    10681 gtacgctggt tacaacggca acttgccggg caagaattgc tggaaacccg ggctactcgt
    10741 atcttaaacg gtgagcgtgg ctctaatgtg ttgggaacca tctatgaatg gccgcccaga
    10801 accagcagtg cgctggatac gctgcttcgt gaaattcaga acgcacgcga acaacacagc
    10861 cgtcttgata cgctgatccg ctcttatgcc gcacaggacg tgaaaaccgg cctcaataac
    10921 cgactctttt tcgataatca gttagcaacg ttactggaag atcaggagaa agtaggtacc
    10981 cacgggatcg tgatgatgat tcgtctgccg gatttcaata tgttgagcga tacctggggg
    11041 cacagccagg ttgaagaaca gttcttcact ctgacgaatc tgctgtcgac atttatgatg
    11101 cgctaccctg gcgcactgct ggcgcgttac caccgcagtg attttgctgc gctgttaccg
    11161 caccggacgt taaaagaggc agagagcatc gccggtcagt taatcaaagc cgtcgatacc
    11221 ttgccgaaca ataaaatgct cgatcgcgac gatatgatcc acattggtat ctgcgcctgg
    11281 cgtagtggtc aggataccga gcaggtaatg gaacatgcag agtctgccac gcgtaatgcg
    11341 ggattgcagg gcggcaatag ctgggctatt tacgatgact cgttgcctga aaaaggacgc
    11401 ggtaatgttc gctggcgtac gcttatcgag caaatgctca gtcgcggcgg cccgcgcctt
    11461 tatcaaaaac cggcggttac tcgcgaaggt caggttcatc atcgcgaact catgtgccgc
    11521 atctttgatg gtaatgaaga ggttagctcg gcggagtata tgccgatggt cttgcagttt
    11581 ggcttatcgg aagagtatga ccgtctgcaa atcagccgtc ttattccact attgcgttac
    11641 tggccagagg aaaatctggc gattcaggtt accgttgagt cgctgattcg cccgcgtttt
    11701 cagcgttggc tgcgcgatac gttaatgcaa tgtgaaaaat cacaacgaaa acgcataatt
    11761 attgaacttg cagaggccga tgtaggtcaa catatcagtc gtttacaacc tgttattcgt
    11821 ttagtgaatg ctttaggggt acgggtagcc gtcaaccagg ctggtttgac gctggtaagc
    11881 accagttgga tcaaagaact taatgttgag ttactcaagc tccatccggg gctggtcaga
    11941 aacattgaga agcgaacgga gaaccagctg ctggttcaaa gcctggtgga agcctgctca
    12001 gggaccagca cccaggttta cgccaccggt gtgcgttcgc gaagcgagtg gcagaccctg
    12061 attcagcgcg gtgttacagg cgggcaaggg gattttttcg cgtcctcaca gccacttgat
    12121 actaacgtga aaaaatattc acaaagatac tcggtttaac ctgccgttta atccgttttc
    12181 acgtagaata acgcgcgctg cgtctcatgg gagtgtgctt gtctgctcgc cagattgttg
    12241 cagcacatat gcagatgaat gaccttacgc ggttgcaaac aggcgaggaa tgctgctgat
    12301 gcattaagcc tttctggact caggcagaga tttgtaacaa aggaaacgaa ctgcactaat
    12361 tttcaccgta gcagatgatt tttgcgcctt gtcgctgctg cgtgtggttg gtaaagtaag
    12421 cggattttct tttccgcccc agctttcagg attatccctt agtatgttga aaaaatttcg
    12481 tggcatgttt tccaatgact tgtccattga cctgggtact gcgaataccc tcatttatgt
    12541 aaaaggacaa ggcatcgtat tgaatgagcc ttccgtggtg gccattcgtc aggatcgtgc
    12601 cggttcaccg aaaagcgtag ctgcagtagg tcatgacgcg aagcagatgc tgggccgtac
    12661 gccgggcaat attgctgcca ttcgcccaat gaaagacggc gttatcgccg acttcttcgt
    12721 gactgaaaaa atgctccagc acttcatcaa acaagtgcac agcaacagct ttatgcgtcc
    12781 aagcccgcgc gttctggttt gtgtaccggt tggcgcgacc caggttgaac gccgcgcaat
    12841 tcgtgaatcc gcgcagggcg ctggtgcccg tgaagtcttc ctgattgaag aaccgatggc
    12901 tgccgcaatt ggtgctggcc tgccggtttc tgaagcgacc ggttctatgg tggttgatat
    12961 cggtggtggt accactgaag ttgctgttat ctccttgaac ggcgtggttt actcctcttc
    13021 cgtacgcatt ggtggtgacc gtttcgacga agctatcatc aactatgtgc gtcgtaatta
    13081 cggttctctg atcggtgaag ccaccgcaga acgtatcaag cacgaaatcg gttcggctta
    13141 tccgggcgat gaagtccgtg aaatcgaagt tcgtggccgt aacctggcag aaggtgttcc
    13201 acgcggtttt accctgaact ccaacgaaat cctcgaagca ctgcaggaac cgctgaccgg
    13261 tattgtgagc gcggtaatgg ttgcactgga acagtgcccg ccggaactgg cttccgacat
    13321 ctccgagcgc ggcatggtac tcaccggtgg tggcgcactg ctgcgtaacc ttgaccgttt
    13381 gttaatggaa gaaaccggca ttccagtcgt tgttgctgaa gacccgctga cctgtgtggc
    13441 gcgcggtggc ggcaaagcgc tggaaatgat cgacatgcac ggcggcgacc tgttcagcga
    13501 agagtaatcg gatgcaggca ggggaagcgt ctgtttaccc tgcctggtct gatacgagaa
    13561 tacgcataac ttatgaagcc aatttttagc cgtggcccgt cgctacagat tcgccttatt
    13621 ctggcggtgc tggtggcgct cggcattatt attgccgaca gccgcctggg gacgttcagt
    13681 caaatccgta cttatatgga taccgccgtc agtcctttct actttgtttc caatgctcct
    13741 cgtgaattgc tggatggcgt atcgcagacg ctggcctcgc gtgaccaact agaacttgaa
    13801 aatcgggcgt tacgtcagga actgttgctg aaaaacagtg aactgctgat gcttggacaa
    13861 tacaaacagg agaacgcgcg tctgcgcgag ctgctgggtt ccccgctgcg tcaggatgag
    13921 cagaaaatgg tgactcaggt tatctccacg gttaacgatc cttatagcga tcaagttgtt
    13981 atcgataaag gtagcgttaa tggcgtttat gaaggccagc cggtcatcag cgacaaaggt
    14041 gttgttggtc aggtggtggc cgtcgctaaa ctgaccagtc gcgtgctgct gatttgtgat
    14101 gcgacccacg cgctgccaat ccaggtgctg cgcaacgata tccgcgtaat tgcagccggt
    14161 aacggttgta cggatgattt gcagcttgag catctgccag cgaatacgga tattcgtgtt
    14221 ggtgatgtgc tggtgacctc cggtctgggc ggtcgtttcc cggaaggcta tccggtcgcg
    14281 gttgtctctt ccgtaaaact cgatacccag cgcgcttata ctgtgattca ggcgcgtccg
    14341 actgcagggc tgcaacgttt gcgttatctg ctgctgctgt ggggggcaga tcgtaacggc
    14401 gctaacccga tgacgccgga agaggtgcat cgtgttgcta atgaacgtct gatgcagatg
    14461 atgccgcagg tattgccttc gccagacgcg atggggccaa agttacctga accggcaacg
    14521 gggatcgctc agccgactcc gcagcaaccc acgacaggaa atgcagctac tgcgcctgct
    14581 gcgcccacac agcccgctgc taatcgctct ctacaaaggg ctacgccgcc gcaaagtggt
    14641 gctcaaccgc ctgcgcgtgc gccgggaggg caatagtggc gagctatcgt agccagggac
    14701 gctgggtaat ctggctctct ttcctcattg cgcttttgct gcaaatcatg ccctggccgg
    14761 ataacctgat tgttttccgg ccaaactggg tgttactcat cttgttgtat tggatcctgg
    14821 ccttgcccca tcgcgtaaat gtgggcacag gttttgtgat gggtgccata ctggatctga
    14881 tcagcggctc gacgcttggc gtacgcgtat tggcgatgag catcattgct tacctggtgg
    14941 cgctgaaata ccagcttttc cgcaacctcg cattatggca gcaggcgctg gtcgtcatgt
    15001 tgctttcgct ggtggtggat attattgttt tctgggcaga gtttttagtg attaacgtct
    15061 ctttcagacc ggaagtgttc tggagtagtg tagtcaatgg ggtgctctgg ccgtggattt
    15121 tcttgctgat gcgtaaagtc cgtcagcagt ttgcagtgca ataaaggttt ctatgacttc
    15181 tctgtattta gcttccggtt ctccgcgtcg tcaggagtta cttgcgcaac ttggcgtgac
    15241 ctttgaacgt attgttacgg gcattgagga gcagcgtcag ccgcaggaga gcgcgcagca
    15301 gtatgttgtg cgtctggcgc gcgagaaagc acgggcaggt gtcgcgcaaa cggcgaagga
    15361 tctcccggtg ctgggtgcgg atactatcgt tatcctgaac ggagaagtgc tggagaaacc
    15421 gcgcgacgca gagcatgcgg cgcagatgtt gcgcaaatta tcgggtcaga cccatcaggt
    15481 gatgacggca gtggcgttgg ccgacagccg gcacattctc gattgcctgg tggtcaccga
    15541 tgtgactttc agaacgttaa cagacgaaga catcgcgggc tatgtcgcca gcggtgaacc
    15601 gttagataaa gcaggtgcat acggtattca ggggctgggt ggctgttttg tcaggaagat
    15661 aaatggcagc tatcaagccg tagtcggctt accgctggtt gaaacgtatg aattattaag
    15721 taattttaac gcactgcgtg agaaaaggga taaacatgac ggctgaattg ttagtaaacg
    15781 taacgccttc ggaaacgcga gtggcgtata ttgatggcgg tattctgcag gaaattcata
    15841 ttgaacgtga ggcgcgacgc ggaatagtag gcaatatcta caagggtcgt gtaagtcgtg
    15901 tacttccggg tatgcaggcg gcttttgtag atattgggct ggataaagcc gcgtttcttc
    15961 atgcatctga catcatgccg cacaccgaat gtgtggcggg tgaagaacaa aagcaattca
    16021 cggtgcgcga catctcggaa ctggttcgtc aggggcaaga tctgatggtg caggtggtga
    16081 aagatccgct tggcactaag ggggcgcgcc tgaccaccga tatcacgctg ccttctcgct
    16141 atctggtctt tatgccaggg gcttctcacg ttggggtttc ccaacgaatt gaaagcgaat
    16201 cagaacgtga acgcctgaaa aaagtggtcg cagagtattg cgacgagcag ggcgggttta
    16261 tcatccgtac cgcagcggaa ggggttggcg aggctgaact ggcctccgat gccgcttatc
    16321 tgaaacgcgt ctggaccaaa gttatggagc gtaaaaagcg cccgcagacc cgttatcagc
    16381 tgtacggcga actggcgttg gcgcagcgtg ttctgcgtga tttcgccgat gccgaactgg
    16441 accgcattcg cgttgactca cgcctgactt acgaagcgtt acttgagttc acctcggagt
    16501 acattcccga gatgaccagc aagctggagc attacaccgg acgccagccg attttcgatc
    16561 tctttgatgt cgaaaacgaa atccagcgag cgctggaacg caaagttgaa ctgaaatccg
    16621 gtggttatct gattatcgac cagaccgaag cgatgaccac cgtggacatc aataccggag
    16681 cgtttgtcgg tcatcgcaat ctggacgaca ccattttcaa taccaatatt gaagcgacgc
    16741 aggctatcgc tcgccagtta cggttgcgta atctgggcgg gattatcatt attgatttca
    16801 tcgatatgaa taatgaagat caccgccgcc gagtgctgca ctcgctggag caggcgttga
    16861 gcaaagaccg ggtgaaaacc agcgttaatg gtttttcggc gctggggctg gtggagatga
    16921 cgcgtaaacg cacccgcgaa agcattgagc acgtactgtg taacgaatgc ccaacctgcc
    16981 acggtcgcgg aacggtgaaa accgtggaaa cggtatgcta tgaaatcatg cgcgagattg
    17041 ttcgtgtaca ccatgcttac gactccgacc gtttcctggt ctatgcttct ccggcagtag
    17101 ccgaagcctt gaaaggcgaa gagtcacact cgctggcgga agtggaaatt ttcgttggca
    17161 aacaggttaa agtacaaatt gaaccgctct ataaccagga gcagtttgac gtcgtaatga
    17221 tgtaaacaga tgctgggccg ccatccggca aagggttttt gagtcacatt tttagcagac
    17281 aaggagtgac gggtgaggcg attgccgggg attttactgc ttactggagc cgcgctcgtt
    17341 gtgatcgctg ccctgctggt tagcggcctg cgtattgctt taccgcatct tgacgcctgg
    17401 cgtccggaaa tcctcaacaa aatagaatcc gcgactggca tgccggtaga agccagtcag
    17461 ctctcagcca gctggcagaa ttttggcccg acgcttgaag cgcacgacat ccgtgcagaa
    17521 ctaaaggatg gcggcgaatt ttcggttaaa cgcgttactc tggcgctgga tgtctggcag
    17581 agcctgttac atatgcgctg gcagtttcgc gacctcactt tctggcagct gcgctttcgc
    17641 accaacactc ctatcaccag cggtggtagc gatgacagtc tggaagccag tcacatcagc
    17701 gatctgtttc ttcgtcaatt tgaccatttc gatctccgcg acagtgaagt cagtttcctg
    17761 acgccatccg gtcagcgcgc cgagctggcg atcccacaac tcacctggct gaacgatcca
    17821 cgtcgacacc gtgcggaagg cctggtaagc ctctccagcc ttaccggaca gcacggcgtg
    17881 atgcaggtgc gcatggattt gcgcgatgat gaggggttgt taagcaatgg tcgcgtctgg
    17941 ctccaggcgg atgacatcga cctgaagccg tggctcggta aatggatgca ggacaatatt
    18001 gcgctggaaa cggcgcagtt ctcccttgaa ggctggatga cgatcgacaa aggcgatgta
    18061 accggcggtg acgtctggct gaaacagggt ggtgccagct ggttgggcga gaaggaaacg
    18121 catacgctgt cggtggataa tctgaccgcg catattacgc gtgaaaatcc gggatggcag
    18181 ttctctattc ccgatacacg gatcacgatg gacggcaaac cctggccgag cggagcattg
    18241 acgctggcct ggataccgga acaggacgtt ggcggcaaag acaataaacg cagtgacgaa
    18301 ctccggattc gcgccagtaa tctggagctg gcaggcctgg agggcatacg cccgctggcc
    18361 gcgaaacttt cacctgcact gggtgatgtt tggcgctcta cacaaccgag cggcaagatt
    18421 aacactctgg cgctggatat cccgcttcag gcggcagaca agacccgttt tcaggcatcg
    18481 tggagcgatc tggcctggaa gcaatggaaa ttattaccgg gtgcggaaca cttctccggg
    18541 acgctttccg gcagcgttga aaatggtttg cttaccgcgt cgatgaagca ggcaaagatg
    18601 ccttacgaaa cggtattccg tgcgccactg gaaatcgccg acggccaggc aactataagc
    18661 tggctgaaca atgacaaagg tttccagctg gatgggcgta atattgacgt taaagccaaa
    18721 gccgtccatg cgcgcggcgg ttttcgttac ctgcaacctg ctaacgatga accctggctg
    18781 ggtattctgg ctggcatcag taccgatgat ggttcacaag cctggcgcta tttcccggaa
    18841 aacttgatgg gtaaagacct ggttgattac ttaagtggcg cgattcaggg cggtgaagcg
    18901 gataacgcga cgctggttta tggtggcaat ccgcaactct tcccctataa acacaacgaa
    18961 ggtcagtttg aagtgctggt gccgctgcgc aacgcgaagt ttgccttcca gccggactgg
    19021 cctgcattaa ctaaccttgg tattgaactg gactttatta acgacggttt atggatgaaa
    19081 accgatggcg ttaatctggg cggcgtgcgc gcgagtaatc tcaccgcagt gatccctgac
    19141 tactcaaaag aaaaactgct gattgacgct gacattaaag gtccgggtaa agccgttggc
    19201 ccttactttg atgagacacc gctgaaagat tctctgggtg cgaccctgca agaactccag
    19261 ctcgacggcg atgtgaatgc tcgcttacat cttgatatcc cgctgaacgg cgagctggta
    19321 accgcgaaag gtgaagtgac gctgcgtaat aacagtctgt ttatcaaacc actcgacagc
    19381 accctgaaaa atttgagcgg taaattcagc tttatcaatg gcgatctgca aagtgaacca
    19441 ctgacagcaa gctggtttaa tcagccgttg aacgtggatt tttccaccaa agaaggggca
    19501 aaagcctacc aggtcgcggt gaatctcaac ggtaactggc aaccggcgaa aaccggcgtt
    19561 ctgcctgcag cggtgaacga agcattgagt ggcagcgtgg cgtgggatgg taaagtgggc
    19621 attgttctgc cttatcatgc tggcgcgacg tataacgtag agctaaacgg cgatttgaag
    19681 aatgtgagca gtcacttacc ttcaccgtta gccaaacctg cgggtgaacc actgccggta
    19741 aacgttaagg ttgatggcaa tctcaacagc tttgaattaa ccggacaggc tggtgcggat
    19801 aatcatttca atagccgctg gttgctcggt caaaagctga cgctcgaccg tgctatttgg
    19861 gcggcagaca gtaaaacgct cccgccgttg ccggaacaaa gtggtgttga actcaatatg
    19921 ccgccgatga atggtgccga gtggctggcc ctgtttcaga aaggtgcggc ggagagtgtc
    19981 ggtggtgcag cgagtttccc acaacacata acgttacgta cgcctatgtt gtcgctggga
    20041 aatcagcaat ggaataacct gagtattgtt tcgcaaccga cggcaaatgg cacccaggtt
    20101 gaggcgcagg ggcgtgaaat caacgccacg ctggcgatgc gtaataacgc gccgtggctg
    20161 gcgaatatca aatatcttta ttacaacccg agcgtggcga aaactcgtgg tgattcaacg
    20221 ccgtcatcac ctttcccgac aacggaacgc attaacttcc gtggctggtc ggacgcccaa
    20281 atacgatgcg cagagtgctg gttctggggg caaaaattcg ggcgcattga cagtgatatc
    20341 accatttctg gcaatacatt aacgctgacc aatggactga ttgatactgg tttttcgcgg
    20401 ctcactgccg atggtgaatg gattaacaat ccggggaatg aacgtacctc gctgaaagga
    20461 aaactgcgcg ggcagaaaat tgatgccgcc gcagaatttt ttggtgtcac gacgcccata
    20521 cggcagtcgt catttaatgt ggattacgat ttacactggc gtaaagcacc gtggcagcca
    20581 gatgaggcga cgttgaatgg catcattcat actcaactgg gtaaaggcga aattaccgaa
    20641 atcaataccg gacatgccgg gcaattgctg cgcttattga gcgtagatgc cctgatgcgt
    20701 aagctgcgtt ttgatttcag agacactttt ggcgaagggt tctattttga ctccattcgc
    20761 agcaccgcgt ggattaaaga cggcgttatg cacaccgacg acacgctggt ggatggcctg
    20821 gaggcggata tcgccatgaa agggtcggta aatctggtac gtcgcgacct gaatatggaa
    20881 gcggttgtcg caccagagat ttctgcgaca gtgggcgtgg ctgcggcttt tgcggttaac
    20941 cccattgttg gcgcggcagt gtttgccgcc agtaaagtgc tggggccgct gtggagcaaa
    21001 gtctccattt tgcgctatca catttcgggt ccgctggacg atccgcaaat caacgaagtg
    21061 ttgcgccaac cgcgtaaaga aaaagcgcaa tgatttgacg agggcgcgta attgccccaa
    21121 tctcatagga taatcgttgc caaaggccaa cgagccagaa cataaccgta ggctggatag
    21181 ggcgttcacg ccgcatccgg cagccgtaaa aaatcctcta ctgcagtaac taacgagtag
    21241 caaaaacgat gagtcttaac ctggtaagtg aacaattgct agcggcgaac ggcctgaaac
    21301 atcaggactt gttcgtgatc ctcggtcaac tggccgaacg tcgccttgat tatggcgatc
    21361 tctattttca gtcgagctat cacgaatcct gggttttaga agaccgcatt attaaagatg
    21421 gttcttacaa catcgatcag ggcgttggtg tgcgtgcaat cagcggtgaa aaaaccggat
    21481 ttgcttacgc tgaccaaatc agcctgctgg cgctggaaca gagtgcgcaa gcggcgcgca
    21541 ccatcgcccg tgatagcggt gatggcaaag tacagacgct gggcgcggta gagcatagcc
    21601 cgttgtatac ctcggtagat ccgctgcaaa gcatgagccg tgaagagaag ctggatatcc
    21661 tgcgtcgcgt cgataaggtt gcccgcgaag cggacaagcg cgtacaggaa gtgactgcca
    21721 gcctcagtgg tgtctatgaa ttaattctgg ttgcggccac cgacggcacg ctagcggcgg
    21781 atgtccgtcc gctggtgcgt ctttccgtga gcgttctgat cgaagaagat ggcaaacgcg
    21841 aacgcggtgc cagtggcggc ggcggtcgtt ttggttatga gttcttcctt gccgatctcg
    21901 acggcgaagt ccgtgcggat gcatgggcaa aagaagcagt acgtatggcg ctggtcaatc
    21961 tttctgccgt tgctgcacca gcgggcacca tgccggtagt acttggcgca ggttggccgg
    22021 gcgtgctgtt gcatgaagcg gtaggtcacg gtctggaagg cgacttcaac cgccgtggca
    22081 cttcagtatt tagtggacag gtcggggagc tggtggcttc agaactgtgt accgtggttg
    22141 atgacggtac gatggtcgat cgccgtggtt cggtggcgat tgatgacgaa ggtacgccag
    22201 gccagtacaa cgtgctgatt gagaacggca ttctgaaagg ctacatgcag gataaactca
    22261 acgcgcgttt gatggggatg acgccgactg gcaacggtcg ccgtgaatcc tacgcccatc
    22321 tgcccatgcc gcgtatgacc aacacctata tgctgccggg taaatcgacc ccgcaggaaa
    22381 ttattgaatc cgttgagtac ggtatctatg caccgaactt tggtggcggt caggtggata
    22441 tcacctccgg caaattcgtt ttctccactt cagaagcata tctgattgaa aacggtaaag
    22501 taacgaagcc ggtgaaaggc gcaacgttga ttggttccgg tatcgaaacc atgcagcaga
    22561 tttcgatggt tggcaacgac ctgaaactgg ataacggcgt gggtgtctgc ggtaaagaag
    22621 ggcaaagttt gccggttggc gtgggccagc caacgctgaa agttgataac ctgactgttg
    22681 gcggtactgc gtaataattt cagtattttc agaaggataa ttaatccttc ctcgtgcacg
    22741 aacggtcccc tcgccccttt ggggagaggg ttagggtgag gggaacccgt tggcacaggt
    22801 ttgtacaacg taacagtaca atatgaatta cttctctttc ccgcgcccgt gcatctcctg
    22861 aaacaattta ccgacctcaa caaaataatc cgtcagcgag ttgatcacga cctgtacctt
    22921 cagcggcagc ttatcttttt cggtatataa cgcataaacc gggcgtggat ctgactgata
    22981 acgcggcagc aggatctcca gctccccacg attgatctcg ttgatcaccc acatcagcgg
    23041 cacgtaggcg atcccggcac ccgccgttag ccagcgcacc agcgtcatcg gatcattagt
    23101 cacaaatctc ccttgcggga tcaggcgagt cgagatccct tccggtgcaa tcagttcaaa
    23161 ttcattgtcg ggccgcacgc tgtattcaag ccatgaatgg ctacttaaat cggcgggttt
    23221 ttccggtatg ccgtattgtg tgagatagct tttcgcggcg cacaccacca ttggcatcgc
    23281 gccaagacgg cgggaaaaca ggctggaatc ctgcaacgcg ccgacgcgga tcaccacatc
    23341 cagaccgtcg gcaatcaggt cgggggctgg aattccggta accagattga cgctcaaacc
    23401 tgggtattct ttcagcattt tggctgtcag cccggcgaga acattttgtg ccatagttga
    23461 agaacagcca atgcgtagcg tcccgatggg ggtgttattg aaggcataca gttgctcatg
    23521 aacatcctgc acttcatgaa gcatacgacg gcagccctgg tagtaaattc taccggcttc
    23581 ggtcaggcca atgctgcgtg tgctacggtt taacagcttt acctgcaact catcttccag
    23641 ttttgacacc gtctgactga tggacgaaac gctcatctgt agctgtctgg cggcggcggt
    23701 aaaagagcca aattcaacta ctttggcaaa caccgacatg cgttttagtc gttccattat
    23761 tcactctgac ttaaaagtga tttagatcac ataatataga taacagcata acagttacgc
    23821 taatatatta aatatcaatc tacagcaatg ttgctctcgc ccggctttcc atgcccttct
    23881 ctgcggcgac agatgctgaa aataataacg cctgctctct ctatacaacc aaggtcaaca
    23941 tgagtctgtt tcccgttatc gtggtgtttg ggctgtcctt cccaccgata ttttttgaat
    24001 tgcttttatc actggcgatt ttctggctgg tgtgccgggt acttgtgcca acaggtatct
    24061 acgactttgt ctggcatccg gcgttgttca acaccgcgct ctattgctgc ttgttttatt
    24121 tgatatcgcg actgttcgtt tgaggttgaa gtgaaaacac taataagaaa attctcccgt
    24181 acggccatca cggtcgtatt agtcattctg gccttcatcg caatttttaa tgcctgggtc
    24241 tattacaccg aatccccctg gacgcgtgac gcgcgcttta gcgctgacgt cgttgcgatc
    24301 gcgccggacg tttctggact cattacccag gtgaatgttc ataaccagct ggtgaaaaaa
    24361 ggacaggttc tgttcaccat cgaccagccg cgctatcaaa aggcgcttga ggaagcgcaa
    24421 gccgatgttg cttattatca ggtactggca caggagaaac gccaggaggc cggacgtcgt
    24481 aaccgtctcg gtgtgcaggc gatgtctcgc gaagagatcg accaggccaa caacgtacta
    24541 caaacggttc tgcatcagtt agcgaaagcg caggcgaccc gcgatctggc aaaactggat
    24601 cttgaacgca cggtgatccg cgcgccagca gatggctggg tgaccaacct caacgtctat
    24661 accggtgagt ttattactcg tggatcaacg gcggttgcgc tggtgaaaca gaactctttc
    24721 tatgtactgg cctatatgga agaaactaag ctggaagggg tgcgccctgg gtatcgtgca
    24781 gagatcacgc cgcttggcag taacaaagtg ctgaaaggga ctgttgatag tgttgccgct
    24841 ggggtcacca acgccagcag cacgcgtgac gacaaaggga tggcgaccat cgactctaac
    24901 cttgaatggg tgcgtttggc gcaacgtgtt ccggttcgta ttcgtctcga caaccagcaa
    24961 gagaacatct ggcctgcggg caccactgct acagtggtgg tcactggcaa acaagatcgc
    25021 gacgaaagcc aggattcgtt cttccgtaaa atggcccatc gcctgcgtga gtttggttaa
    25081 tcacgatgga tattttctcc attgctaacc aacatattcg ctttgcggta aaactggcga
    25141 ccgccattgt actggcgctg tttgttggct ttcacttcca gctggaaacg ccacgctggg
    25201 cggtactgac agcggcgatt gttgccgccg gtccggcctt tgctgcggga ggtgaaccgt
    25261 attctggcgc tattcgctat cgtggctttt tgcgcatcat cggcacattt attggctgta
    25321 ttgccggact ggtgatcatc attgcgatga tccgcgcacc attattgatg attctggtgt
    25381 gctgtatctg ggccggtttt tgtacctgga tatcctcgct ggtacgaata gaaaactcgt
    25441 atgcgtgggg gctggccggt tataccgcgc tgatcattgt gatcaccatt cagccggaac
    25501 cattgcttac gccgcagttt gccgtcgaac gttgtagcga gatcgttatc ggtattgtgt
    25561 gtgcgattat ggcggatttg ctcttttctc cgcgatcgat caaacaagaa gtggatcgag
    25621 agctggaaag tttgctggtc gcgcaatatc aattaatgca actctgtatc aagcatggcg
    25681 atggtgaagt tgtcgataaa gcctggggcg acctggtgcg acgcaccacg gcgctacaag
    25741 gcatgcgcag caacctgaat atggaatctt cccgctgggc gcgggccaat cgacgtttaa
    25801 aagcgatcaa tacgctatcg ctgacgctga ttacccaatc ctgcgaaact tatcttattc
    25861 agaatacgcg cccggaattg atcactgata ctttccgcga attttttgac acgccggtag
    25921 aaaccgcgca ggacgtccac aagcagctca aacgcctgcg aagagttatc gcctggaccg
    25981 gggaacggga aacgcctgtc accatttata gctgggtcgc ggcggcaacg cgttatcagc
    26041 ttctcaagcg cggcgttatc agtaacacaa aaatcaacgc caccgaagaa gagatcctgc
    26101 aaggcgaacc ggaagtcaaa gtagagtcag ccgaacgtca tcatgcgatg gttaacttct
    26161 ggcgaaccac actttcctgc attctgggaa cgcttttctg gctgtggacg ggctggactt
    26221 ccggcagtgg tgcaatggtg atgattgcgg tcgtgacgtc actggcaatg cgtttgccga
    26281 atccacgcat ggtggcgatc gactttatct acgggacgct ggccgcgctg ccgttagggc
    26341 tgctctactt tttggtgatt atccctaata cccaacagag catgttgctg ctgtgtatta
    26401 gcctggcagt gctgggattc ttcctcggaa tagaagtaca gaaacggcga ctgggctcga
    26461 tgggggcgct ggccagcacc ataaatatta tcgtgctgga taacccgatg actttccatt
    26521 tcagtcagtt tctcgacagc gcattagggc aaatcgtcgg ctgtgtgctc gcgttcaccg
    26581 ttattttgct ggtgcgggat aaatcgcgcg acaggactgg acgtgtactg cttaatcagt
    26641 ttgtttctgc cgctgtttcc gcgatgacta ccaatgtggc acgtcgtaaa gagaaccacc
    26701 tcccggcact ttatcagcag ctgtttttgc tgatgaataa gttcccaggg gatttgccga
    26761 aatttcgcct ggcgctgacg atgattatcg cccaccagcg cctgcgtgat gcgccgatcc
    26821 cggttaacga ggatttatcg gcgtttcacc gacaaatgcg ccgcacagca gaccatgtga
    26881 tatctgcccg tagcgatgat aaacgtcgtc ggtactttgg tcagttgctg gaagaactgg
    26941 aaatctacca ggaaaagcta tgcatctggc aagcgccacc gcaggtgacg gaaccggttc
    27001 atcggctggc ggggatgctc cataagtatc aacatgcgtt gaccgatagt taagtcaaaa
    27061 ccgacgccaa aagcgtcggt tttttcatgg ctatacttag cgatgcacgg cagaactcgc
    27121 cgcgaaacgt gacggtggca acagatgaat atttatacct ttgattttga tgagattgag
    27181 agtcaggagg atttttatcg tgactttagc caaacctttg gtctggcgaa agataaggta
    27241 cgcgatctcg actcactatg ggatgtgtta atgaacgatg tcctgccgct accacttgag
    27301 attgaatttg ttcatctggg agagaaaacg cgtcgccgtt ttggcgcgtt aatattgctg
    27361 tttgatgagg cagaggaaga gctggaaggg catttgcgtt ttaatgttcg tcattagcgc
    27421 aaaaaaaagc ccccgaaccg ggggcaatat cgtcggacaa gacgatgagg gtttatttgt
    27481 acagttcagc cgtagcgtgc caggtgtcac cgctacgagc ttcagtaatc tggtaggccg
    27541 ttgcgccttt ctcttccgct tttttgttca gcatttcacg catatccatt ggcgaagacg
    27601 ccacaccact tacggatacg gtcccgattg cttcacgatt ttgtgcttgt gcagcatcaa
    27661 tggagtcggc agcgaatgca ccgaaagaga gaacagaaag tacgcttaat gcagcaacag
    27721 tggttttgat tttcatgatt tttacctcga cataatcttt tagctgggcc tttgtttcgt
    27781 gacccatatc acaaaatcaa gtatacacta attattgagc taattaatac cgt
//
LOCUS       DAZFAB010000053        27711 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZFAB010000053 DAZFAB010000000
VERSION     DAZFAB010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27711
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.053"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..234)
                     /locus_tag="ACHJWA_002642"
     CDS             complement(<1..234)
                     /locus_tag="ACHJWA_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO4309217.1"
                     /translation="MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVI
                     KKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSK"
     gene            complement(460..1785)
                     /gene="rimO"
                     /locus_tag="ACHJWA_002643"
     CDS             complement(460..1785)
                     /gene="rimO"
                     /locus_tag="ACHJWA_002643"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFO4309218.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            1998..2381
                     /gene="bssR"
                     /locus_tag="ACHJWA_002644"
     CDS             1998..2381
                     /gene="bssR"
                     /locus_tag="ACHJWA_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFO4309219.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            2492..3607
                     /gene="yliI"
                     /locus_tag="ACHJWA_002645"
     CDS             2492..3607
                     /gene="yliI"
                     /locus_tag="ACHJWA_002645"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFO4309220.1"
                     /translation="MHRQFFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(3604..4230)
                     /gene="gstB"
                     /locus_tag="ACHJWA_002646"
     CDS             complement(3604..4230)
                     /gene="gstB"
                     /locus_tag="ACHJWA_002646"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFO4309221.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            4476..5678
                     /gene="dacC"
                     /locus_tag="ACHJWA_002647"
     CDS             4476..5678
                     /gene="dacC"
                     /locus_tag="ACHJWA_002647"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO4309222.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            complement(5725..6483)
                     /gene="deoR"
                     /locus_tag="ACHJWA_002648"
     CDS             complement(5725..6483)
                     /gene="deoR"
                     /locus_tag="ACHJWA_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFO4309223.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(6541..7137)
                     /gene="ybjG"
                     /locus_tag="ACHJWA_002649"
     CDS             complement(6541..7137)
                     /gene="ybjG"
                     /locus_tag="ACHJWA_002649"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO4309224.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLLVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            7422..8659
                     /gene="mdfA"
                     /locus_tag="ACHJWA_002650"
                     /pseudo
     CDS             7422..8659
                     /gene="mdfA"
                     /locus_tag="ACHJWA_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
     gene            complement(8700..8981)
                     /gene="ybjH"
                     /locus_tag="ACHJWA_002651"
     CDS             complement(8700..8981)
                     /gene="ybjH"
                     /locus_tag="ACHJWA_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFO4309225.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVAVPQTVYVAPAPAPAYHPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(9067..9882)
                     /gene="ybjI"
                     /locus_tag="ACHJWA_002652"
     CDS             complement(9067..9882)
                     /gene="ybjI"
                     /locus_tag="ACHJWA_002652"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFO4309226.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKMVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKTLHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGNAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(9882..11090)
                     /locus_tag="ACHJWA_002653"
     CDS             complement(9882..11090)
                     /locus_tag="ACHJWA_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4309227.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALVGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSMVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            11174..11710
                     /gene="rcdA"
                     /locus_tag="ACHJWA_002654"
     CDS             11174..11710
                     /gene="rcdA"
                     /locus_tag="ACHJWA_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFO4309228.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(11885..13570)
                     /locus_tag="ACHJWA_002655"
     CDS             complement(11885..13570)
                     /locus_tag="ACHJWA_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFO4309229.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            13840..14217
                     /locus_tag="ACHJWA_002656"
     CDS             13840..14217
                     /locus_tag="ACHJWA_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFO4309230.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(14247..14504)
                     /gene="grxA"
                     /locus_tag="ACHJWA_002657"
     CDS             complement(14247..14504)
                     /gene="grxA"
                     /locus_tag="ACHJWA_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFO4309231.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            14664..14951
                     /locus_tag="ACHJWA_002658"
     CDS             14664..14951
                     /locus_tag="ACHJWA_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFO4309232.1"
                     /translation="MRAIGKLPKSVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            14935..15657
                     /gene="nfsA"
                     /locus_tag="ACHJWA_002659"
     CDS             14935..15657
                     /gene="nfsA"
                     /locus_tag="ACHJWA_002659"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFO4309233.1"
                     /translation="MTPTIELTCGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPSSILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            15718..16620
                     /gene="rimK"
                     /locus_tag="ACHJWA_002660"
     CDS             15718..16620
                     /gene="rimK"
                     /locus_tag="ACHJWA_002660"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFO4309234.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            16708..17184
                     /locus_tag="ACHJWA_002661"
     CDS             16708..17184
                     /locus_tag="ACHJWA_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFO4309235.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            17535..18647
                     /gene="potF"
                     /locus_tag="ACHJWA_002662"
     CDS             17535..18647
                     /gene="potF"
                     /locus_tag="ACHJWA_002662"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFO4309236.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRDNPG
                     IYPPADVRAKLFTLKVQAPKIDRVRTRAWTKVKSGK"
     gene            18742..19875
                     /gene="potG"
                     /locus_tag="ACHJWA_002663"
     CDS             18742..19875
                     /gene="potG"
                     /locus_tag="ACHJWA_002663"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFO4309237.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLYSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            19885..20838
                     /gene="potH"
                     /locus_tag="ACHJWA_002664"
     CDS             19885..20838
                     /gene="potH"
                     /locus_tag="ACHJWA_002664"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFO4309238.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNFGNFLQLTGDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            20835..21680
                     /gene="potI"
                     /locus_tag="ACHJWA_002665"
     CDS             20835..21680
                     /gene="potI"
                     /locus_tag="ACHJWA_002665"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFO4309239.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVLFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAVISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     CARRG"
     gene            21740..22228
                     /locus_tag="ACHJWA_002666"
     CDS             21740..22228
                     /locus_tag="ACHJWA_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFO4309240.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            22269..23396
                     /gene="rlmC"
                     /locus_tag="ACHJWA_002667"
     CDS             22269..23396
                     /gene="rlmC"
                     /locus_tag="ACHJWA_002667"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFO4309241.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQA
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALVERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIALEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKM"
     gene            23676..25019
                     /locus_tag="ACHJWA_002668"
     CDS             23676..25019
                     /locus_tag="ACHJWA_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000399308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFO4309242.1"
                     /translation="MEGVPTMFAKFIDVIQTFLTEPAILIGLLVGIGYALDKKSPIKI
                     ITGMVSAMVGLMMVLFGGFQFSATFKPVAEAVSKAYGVHGYLMDSYAMKAATQIALGD
                     NFGYVGYVFVLAFFTNLLLVLFGRYTGAKGIFLTGNTGVSHSQAVLWLIVFWLGFSWT
                     TSIIIAGILTGVFWAFSTTLIVKPIAKVTKDAGFTIAHNQMLGLWFFSKFAHKFGDPE
                     KHDAENLKLPGWLAIFNHNVTAIAIVMTLFVGGFLLSTGIDNVQLMAKGKPWYIYIIN
                     LGLQFSMYMVILLQGVRMMVGEINGSFKGWQDRFIPNAIPAVDVAALLPFSPNAATLG
                     FVFCTFGTIFSMGILLLVHSPIMVLPGFVPLFFSGGPIGVLANRMGGYRSVIICTFLL
                     GIIQTFGTVWAIPLTGLAENGVGWTGIFDWATVWPAICEVLKFIAATFHLGPYAG"
     gene            25041..25361
                     /locus_tag="ACHJWA_002669"
     CDS             25041..25361
                     /locus_tag="ACHJWA_002669"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO4309243.1"
                     /translation="MKDTINILFVCGYGVGSSVMLQTVVKKALTKYDFKVEMEHTAAG
                     EVGGFTDWADIFAISKKLLDVVSLDPKQGQYLIPIENIMDGETIGQQIYEVVQQHFPH
                     LINQ"
     gene            25373..26854
                     /locus_tag="ACHJWA_002670"
     CDS             25373..26854
                     /locus_tag="ACHJWA_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HFO4309244.1"
                     /translation="MRAIILLFDSLNKRYLPPYGDALTKAPNFQRLAAHAATFENSYV
                     GSMPCMPARRELHTGRCNFLHREWGPLEPFDDSMPELLKKAGIYTHLISDHLHYWEDG
                     GGNYHNRYSSWEIVRGQEGDHWHASVAQPPIPEVLRVPQKQTGGGVSGLWRHDWANRE
                     YIQQEADFPQTKVFDAGCAFIHKNHAEDNWLLQIETFDPHEPFHTTEEYLSLYEDNWD
                     GPHYDWPRGRVQESDEAVEHIRCRYRSLVSMCDRNLGRILDLMDEHDLWRDTMLIVGT
                     DHGFLLGEHGWWAKNQMPYYNEVANNPLFIWNPRSGVKGERRQALVQMIDWAPTLYDF
                     FQQPVPPDVQGQPLAKTVSHDEPVRSSAMFGVFSGHANVTDGRYVYMRAALPGREDDI
                     ANYTLMSCKMNSRYPVDEMRALSLAPPFRFTKGLQVLRIPAQEKYKGLNQFGHLLFDL
                     QNDPQQLHPIHDDVIESRMIALLIQLMKDNDAPPEQFQRLGLA"
     gene            complement(26905..27636)
                     /gene="artJ"
                     /locus_tag="ACHJWA_002671"
     CDS             complement(26905..27636)
                     /gene="artJ"
                     /locus_tag="ACHJWA_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFO4309245.1"
                     /translation="MKKLVLAALLASCAFGASAAEKINFGVSATYPPFESMDANNQIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPDRSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNSRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQQISDQWFPQ"
BASE COUNT         6730 a         6693 c         7209 g         7079 t
ORIGIN      
        1 cttggaccaa ccatcataaa aaaacacatg atttgctgat ttaaaaaaga taattgttct
       61 ggctccacca ctctttcctt gttggagggg taaccgtttt ttgatgacac cgctacctaa
      121 atcagcctcc catatgcctt gcatcacttc ttcagcggca gccatgagaa ctttatcgtg
      181 aataccaagt tttttcgtgc tttggctgaa tcgccttgcc ttatacacac ccatacatcc
      241 atccttgtgg taatgtttac tatagtaacc attacgctta tttcaagtaa ttgcgatcaa
      301 aactttaatt tggtgtgaaa aagtgagagt tgcctcgagt acgattcatt tttctcgatt
      361 tatgcgtcag gggattttgt gcgtaagggt aggacatcgg gaagggtagt agaggcagat
      421 cgaagaacaa tatccttgtt agatctgcct ttagattaat taaacccggc taccccacaa
      481 atcgtactca tcggcgtgct cgactttcac acgcaggata tcacccggct taacgttggt
      541 ttcaccattg agataaaccg caccgtcgat ttccggtgca tctgccatgc tgcgaccaat
      601 cgcgccttct tcgtccactt cgtcgataat caccagaatt tcacggccca ctttctcttg
      661 caggcgctcg gcggaaatct gctgctgcaa ctgcatgaaa cggttccagc gttcttcttt
      721 cacttcttcc ggaacctggt caggcagggc attggcgtct gcaccttcaa ccgggctgta
      781 tttaaagcag ccaacgcgat ccagacgcgc ttctttcagg aagtcgagta gcatctggaa
      841 atcttcttcc gtctcgccag ggaagccgac aataaaggtt gagcgtaggg tcagttccgg
      901 gcagatttcg cgccactgtt tgatgcgcgc cagttggcga tctacagaac ccggacgctt
      961 catcagtttg agaatgcgcg ggctggcgtg ctgcaacgga atgtccagat acggcaggat
     1021 tttgccttct gccatcagtg ggatgacgtc gtccacatgc ggataagggt aaacgtagtg
     1081 cagacgtgtc cagatcccca gtttcgataa ctgttcgcac aggctgacca tgctggtttt
     1141 taccggctcg ccgttgtgga agccagtacg atgtttaaca tcaacgccat aggcggaagt
     1201 atcctgcgag atcaccagaa tctctttaac gcccgcatct accagacgtt tcgcttcact
     1261 taatacttcg ccaatcggac ggctcaccag gtcgccgcgc atagacggaa taatgcagaa
     1321 ggtgcagcgg tgattacagc cttcagaaat tttcagatag gcataatgac gcggcgtcag
     1381 tttgacacct tgttctggca ccaggctcag gaatgggttg tgtttcggtt ttggcacgta
     1441 gtgatgaacg tgctccagaa cctgctcata gctatgaggc ccggtgattt ccagcacttt
     1501 cgggtggact tcgcggatct gatcttcttt tgcccccaga caaccggtca caattacctt
     1561 gccgttttca ttcaacgctt caccaatggc ttccagtgat tcttgtaccg cgctgtcaat
     1621 aaagccgcag gtgttgacga tcaccatgtc cgcatcgtca tagctcggta ccacgtcata
     1681 accttcagtg cggagttcgg tgagaatacg ctctgaatcg acaaggtttt tcggacagcc
     1741 aagggaaaca aagccgattt tcggctgggg agttactttg ctcatagctt aaaaaatatt
     1801 cagttacagg aaaggtcagg gcagggattc tacagagttc tggataaaat ttgtatcgca
     1861 atctcattcg ctggcggagg cgaaggaaat gtaaattttg ttaattcggc gtgaagaatt
     1921 gatcctggac agcattatgc tcaaaaaata gccatactat ttaattgcaa caaggctgga
     1981 aagaggagga tcgaagtatg ttcgttgaca gacagcgaat cgatctgctg aaccggttga
     2041 tcgacgcacg cgttgacctc gccgcatacg tgcaactgag gaaggcaaaa ggatacatgt
     2101 ccgtcagcga aagcaatcat ctacgagata acttttttaa actgaatcgc gaactgcacg
     2161 ataaatcgct gcggttgaat cttcatctgg atcaggaaga gtggagtgct cttcatcatg
     2221 ctgaagaagc attagcgaca gccgcagtat gtttgatgag tgggcaccat gattgcccga
     2281 ctgttattac cgtcaacgcc gataagcttg aaaattgtct gatgagctta acgctgagta
     2341 tccagagcct gcagaagcac gccatgcttg agaaggcctg aaaactaagg gggagaaagc
     2401 gtctccccct tcatgtttaa gtttttgtaa aaatgaattt gttatctcct ccactgacta
     2461 cgctttaagc cagagtcaat ccggaggcgt tatgcatcga caattctttt tccttgtgcc
     2521 ccttatttgt ctttcttccg ctctctgggc ggctcctgca acggtaaatg tcgaagtact
     2581 gcaagacaaa ctcgaccatc cctgggcact ggccttttta cccgataacc acggtatgtt
     2641 aatcactctg cgcggcggcg agttgcgtca ctggcaagca ggaaaaggat tatctgcgcc
     2701 gctttccgga gttccggacg tttgggcgca cgggcagggc ggcctgctgg acgtggtttt
     2761 agcgcctgat tttgctcagt ctcgccgcat ctggttaagt tattccgaag ttggcgatga
     2821 tggcaaagcc ggaactgctg tggggtatgg ccgcttaagt gatgatctct caaaagtgac
     2881 cgactttcgc accgtctttc gccagatgcc aaaactgtct accggcaacc attttggcgg
     2941 gcggctggta ttcgacggta aaggttatct ttttattgct ctgggcgaaa acaatcagcg
     3001 cccgacggcg caggatctgg ataaattaca gggcaaactg gtgcgtctga ccgaccaggg
     3061 cgaaatcccg gatgataatc cttttataaa ggaatccggt gcgcgcgccg agatctggtc
     3121 ttatggcatt cgtaatccgc aaggaatggc gatgaatccg tggagtaatg cactgtggct
     3181 gaatgaacat ggcccgcgcg gtggtgatga aattaatatc ccgcaaaaag gcaaaaacta
     3241 cggctggccg ctggcaacct ggggaatcaa ctattcaggc tttaagatac cggaagcgaa
     3301 aggggagatc gtcgccggga ccgagcaacc tgttttttac tggaaagatt cgcccgccgt
     3361 gagcggcatg gccttctata acagcgataa attcccccag tggcagcaaa aattatttat
     3421 tggcgcgctg aaagataaag atgtcattgt gatgagcgtc aacggcgaca aagtaacaga
     3481 agatggccgt attttaacgg acagagggca gcgaattcgt gatgttcgca ctggacccga
     3541 cggttattta tacgttctca ccgacgagtc cagtggggaa ttacttaaag ttagcccacg
     3601 caattagcta acgggaatca tcaccacttt gcgcactgcg gggcgttcag tgagttgctg
     3661 ataccagcgt tgcagatttg gacgcggtgt ccaggtcagg ccaacattaa acaaattgta
     3721 gatgaacggt gcgatagcga tatcgcccac accaaactcg tcgccagaga accattttac
     3781 ttttgccagt tccgcatcga gcagggcaaa cagggcgtcg cactctttgc aactggcatc
     3841 aatggcggcc tgatcgcgct cttccggtgg tgttctgact aatcccatca ggatcccgcg
     3901 atgagcatta ctgagtgtct ggtttgccca gtccatccat ttttctgctt ccgcacgacg
     3961 tgccggtgag tcgatccaca ggcgtttttg cccgtactgt gccgccagat agcggacaat
     4021 ggcgtttgat tcccaaagaa tgagatcact ttcgtcgtca cgcaacaacg gcaccagccc
     4081 gttagggttc atcgccagaa aatcagcatc gtgatttatc ccaaactcac ggcccgcgag
     4141 aatttgctca taaggtagtt ccagttcttc gagcgtcagc aatacttttt ttacgttggt
     4201 tgaattattc cgaccccaca gcgtaatcat actcacccct tttccgacag gcagccacag
     4261 gctgaacagg actccgatgg tgaagtaaag ttaactttta agcaacagct ggcaaaaaaa
     4321 tgacaccaga agcagcgaag cggcaggata ttgcataaac tttaaaaact ttaccaactt
     4381 acggtttctt taagtttgtg tgtgcgttat taatcaccga acttatcata cggcgatata
     4441 acgtattttt ttgaatggat actcgggtgg catttatgac gcaatactcc tctctccttc
     4501 gtggtcttgc agcgggttct gcatttttat tcctttttgc cccaacggca ttcgcggccg
     4561 aacaaaccgt tgaagcgccg agcgtggatg cgcgtgcatg gattttaatg gattacgcca
     4621 gcggtaaagt gctggcagaa ggcaatgcgg atgagaaact ggatcccgcg agcctgacta
     4681 aaatcatgac cagctatgtg gttgggcagg cgcttaaggc cgataagatt aaactcaccg
     4741 atatggtgac ggtcggtaaa gatgcctggg cgacgggaaa tccggcactg cgtggttcat
     4801 cggtaatgtt cctcaaaccg ggcgatcagg tttcggtggc agacttgaac aaaggtgtga
     4861 ttatccagtc cggtaatgac gcctgtattg cgctggccga ttacgttgcc gggagccagg
     4921 agtcatttat tggtttgatg aatggttatg ccaaaaaact ggggctgacc aacactacct
     4981 tccagacggt gcacggtctg gatgcgccgg ggcagttcag taccgcgcgc gatatggcat
     5041 tgctgggtaa agcattgatc cacgatgtgc cggaagagta cgccattcat aaagagaaag
     5101 agttcacctt caacaaaatt cgtcagccta accgtaaccg tctgctgtgg agcagcaatc
     5161 tgaatgttga tggcatgaag acaggaacca cggcaggcgc gggatataat ctggttgctt
     5221 cggctaccca gggcgatatg cgtttaatct ccgtagtgct gggggcgaaa accgaccgta
     5281 tccgttttaa tgagtctgag aaattattga cctggggttt ccgcttcttt gaaaccgtga
     5341 cgccaattaa acctgatgcc acctttgtga ctcagcgcgt ctggtttggt gataagagcg
     5401 aagtgaatct cggggcaggc gaagcgggct cagtgaccat accgcgtggg cagctgaaaa
     5461 acctgaaggc gagctacacg ttaacggaac cgcagcttac cgcaccgctg aaaaaaggtc
     5521 aggttgtcgg gaccattgat ttccagctta acggtaaatc cattgagcag cgtccgctga
     5581 tcgtgatgga aaatgtggaa gagggcggat tctttggtcg gatgtgggat ttcgtgatga
     5641 tgaaattcca tcagtggttc ggcagctggt tctcttaatc ttctgataac cggatggcgc
     5701 gaaacgtcat ccggttatac gtcattaata catcaactta atacgctgcg tctgcgcgta
     5761 cttcacatac tcatcttccg ggcaacaatc gctcaccaca atatcaaagc gtttcaggtc
     5821 acccatgcgc gccggacgca ccttgccaaa tttactgtgg tcaacaacca gcacatgctt
     5881 ttgcgccatc gacatggccc agtgttttac cggcaactct tcaagattaa aacaggtagc
     5941 gcctttactg acatgcacgc ccgccgcaga ataaaaagcg atatccgggc aaaaattatt
     6001 cagcgtttgc tgaaaatcga tgggtttgaa aatggcgttg ctggcgtgaa attcaccacc
     6061 gcacagaaat gcgcggcaat ggggtttttc tttcagcgcc agaaaggtat ttagcgaata
     6121 acaaacggcg gtaaaaggga tttcattatc aatcgcttca ataatccacg gcgtggtggt
     6181 gccacagtca aaaaagaggg tctgatcggg ttctaccagt gtcgcagcca gttttgccgc
     6241 ccggcgtttt tcttccacca ggcgggattt ttgatcgctt aacaggtaat ggctggcgct
     6301 gcgcggttcc agaacaatat agccgccgag caaaacgacg ggcgcactgt ggttgttcag
     6361 atcgcgacga atcgtcatct ccgaaacccc aagcagggcg gcggcgtctt taagatgtaa
     6421 cttatcgctg cgttttaatt cttgcagcag ctgcccgata cgctcttcgc gacgtgtttc
     6481 cataatccct ctgaatagtt attgaagcga gccgctcaat actacacttt ttagcagaga
     6541 tcagtcacgc acccagcctt tgcggatcgg taatgcaaaa cagaagcgat accacgattg
     6601 cagacgttga tagagtttat gccccatcgc ttgccagata atctgggcac taaggcagcc
     6661 aatcatacct gccagcaatc caccgagcat atccagcggc cagtggacgc caagataaac
     6721 gcgcgaccag gcaatgacga cggccagcac cattaaaagt gagccggacc acaggcgatg
     6781 ccagcataaa aatgccagtg caaaggtgaa aatcaccgta ccgtgatcgc tcgggaatga
     6841 gtcatccgcc gcatgatgca ggaagttata gccgatattt tcgacaaacg gtcggtcgtg
     6901 cggaaaaaga tgtcccatcg tccaggacac caacaggctg accgccagcg cgatagcgat
     6961 ttttatcacc agttgccgtt gtgctgtaag cccccacaac caaagtaccg cggccagcag
     7021 cggcaccacg gtaatcaaat ctttagcaat aaaaatcgcc aacgagatca tccacggagc
     7081 cgagtctggc gtcgcgttaa taagagagaa tagagagaga ttcaaatttt ccagcataac
     7141 ttcccgacgc aaagtgatta aaagggagcc aatacaggca agtcgttgag aataaagtgc
     7201 aggttaaact gggtaaagcg gcatcgtctt atttccctca agcggcctgt ttacggtggg
     7261 tgattgtaac gggcataggt taaataaaac ttaaagaaag cgtagctata ctcgcaataa
     7321 tgtaagaatg tgcttaaccg tggtttcagc tacaaaattc gctttctcgt tagctgcgct
     7381 tttattaaac tctgcgcgat tattattggc gaagaaattg catgcaaaat aaattagctt
     7441 ccggtgccag gcttggacgt caggcgttac ttttccctct ctgtctggtg ctttacgaat
     7501 tttcaaccta tatcggcaac gatatgattc aacccggtat gttggccgtg gtggaacaat
     7561 atcaggcggg cattgattgg gttcctactt cgatgaccgc gtatctggcg ggcgggatgt
     7621 ttttacaatg gctcctgggg ccgttgtcgg atcgtattgg tcgccgtccg gtgatgctgg
     7681 cgggtgtggt gtggtgtggt ttattgtcac ctgtctggca atattactgg cgcaaaacat
     7741 tgaacaattc accctgttgc gcttcttgca gggcataagc ctctgtttca ttggcgctgt
     7801 gggatacgcc gcaattcagg aatccttcga agaggcggtt tgtatcaaga tcaccgcgct
     7861 gatggcgaac gtggcgctga ttgctccgct acttggtcca ctggtgggcg cggcgtggat
     7921 ccatgtgctg ccctgggaag ggatgtttgt cttgtttgcc gcattggcag cgatctcctt
     7981 tttcggtctg caactagcca tgcctgaaac cgccacgcgt ataggcgaga aactgtcgct
     8041 gaaagaactc ggtcgtgact ataagctggt gctgaagaac ggtcgctttg tggcgggggc
     8101 gctggcgctg ggattcgtta gcctgccgtt gctggcgtgg atcgctcagt cgccgattat
     8161 catcattacc ggcgagcagt tgagcagcta tgaatatggt ttgctgcaag tgcctatttt
     8221 cggggcgtta attgcgggta acttgctgtt agcgcgtctg acctcgcgcc gcaccgtacg
     8281 ttcgctgatt attatgggcg gctggccgat tatgattggt ctgttggtcg ctgctgcggc
     8341 aacggttatc tcatcgcacg cgtatttatg gatgaccgcc gggttaagta tttatgcttt
     8401 cggtattggt ctggcgaatg cgggactggt gcgattaacc ctgtttgcca gcgatatgag
     8461 taaaggtacg gtttctgcgg cgatgggaat gctgcaaatg ctgatcttta ccgtcggtat
     8521 tgaaatcagc aaacatgcct ggctgaacgg gggcaacgga ctgtttaatc tcttcaacct
     8581 tgtcaacgga attttgtggc tgtcgctgat ggttatcttt ttaaaagata aacagatggg
     8641 aaattctcac gaagggtaaa aaaatgcctg attgctttgt gcgatcaggc attctcgaat
     8701 taatggtgat ggtcgtcaat ctggtgttcg ataaccatcc cttcacctac gctggcaaga
     8761 tggcgaacat aaggatgcgg gtggtaagcc ggagctggcg caggagccac atagacggtt
     8821 tgcggcacag cgacgctgac cgcttgtgga acgctgactg aatcaggaac cacaaccact
     8881 ttgtatccac tcggcacatc gacggtgaca ctttgcgcca tcgccacacc agtaaagccc
     8941 attaaaagcg cgcccaacag tagacaattt ttcataatca tttcgcctga aataatgcag
     9001 cgggttaatt tccgcgaatt atgcagcctt cattcaggta ttgtgttgcc gggcgtgctg
     9061 ccgtttttat tggtcaaatg gcgcttcgtg tttaagaact ttatcgatca catccagtac
     9121 gccttcacgg ttattggagc ctgcccggta ttttgccgct gcgacgaccg cgttgccggc
     9181 attttccatt gcaaaactaa agcctgcctg acgcagcatc tcaatatcgt taccgccatc
     9241 gccaaagacc accacttcgc tgtcgtctat tccccataat ttctgcagtt ggcgaaggcc
     9301 attggcttta tgtacgccgg ggataatcag atcgatgctg ccgttgccgg tgtggaccgg
     9361 caccataata tcgccgatgg cctcatgtaa tgttttttgt acttgtggaa tcagttcatc
     9421 ggaaagattc aggccaaact taaagaagat atcttctaag ttgtcaaagt tatcgacgta
     9481 ttccagacgg tgataataca tttccgccac cattttcatg gcatcgtcat attttttgag
     9541 cgtataggcg ctattttttc cgcaggcaat aatttccact tccgggcgcg tcagcaaatg
     9601 ttccacgaca gtagtaaacg catccttcga cagctcgcca ttaaaaacat ctttgccttc
     9661 actcactacc cagccgccgt tttcagccac aaaggcaatt tcattagcaa tttcagggaa
     9721 gaaagagatc aactgataat attgattccc gctggcgacc acaaagcgaa tgccttgtgc
     9781 tttcatttgc tgatactgag ccataaaccg ctcacggtta taggtttttt gatcgcttaa
     9841 gaaagtacca tccatgtcta ccgcaattaa tttaatgctc atcaactatt ctccatcgcc
     9901 gtctgcgttt tggtatcggg tttggcgacg gctttcgcga caatagccgc gagaataacc
     9961 agcgccagta caaccagcat tgcactacgt aatccataat gttcgccgag atagcccagc
    10021 agcggcggcc cgacgaggaa agccagataa ccggtcgttg ctaccatact gacgcgggtt
    10081 ggtgcatcgg ggccggtatc gctggcggca gaaatggtca gcgggaagcc cagcgaggca
    10141 cccagtcccc agagtacaac agacacccca gcgacccagg cgctatcgac aaaaataatc
    10201 agcccaatac ccaacgcccc cattagcgca ctggcccgaa ccacggcaac gcgactgtaa
    10261 cggtcgatga accaaccgcc agtgaagcgt ccaacggtca tccccagggt aaaaccggca
    10321 taaatcagcg agccggaagt ggggctaaaa ccgtgaccgt caaccattaa taagggtaac
    10381 cagtcgttgg cagaaccttc ggcaaaggcc atcgccagca ccacaacacc aatcagcagc
    10441 aactggatat cgcgataaaa aggtacgcct ttttcgccat gctgggtgcc atcggcagca
    10501 tttttgcccg taccgtcagg gattgcctga atggcgatat atataggcgc gatgcctacc
    10561 agcgccgcca ataaaatgtg caccgttgcc ggaacgccaa aggccgtcag tgccatcccg
    10621 acaccagcgc ctgccagcgt gcccaggcta taaaaaccgt gcatcatcgg caaaaccgtt
    10681 ttattcattt ctcgctcaac ggcggcacct tcaacgttta tcgccacttc cgcagaacca
    10741 aaacttgccc caaagacgcc gagaccaacg gcaaagagca ggggcgatgt cagccagagt
    10801 gccagactta atatcatcat cccgaccaat gcgcaggaca tggtgaccag gatgacatta
    10861 cgtgtcccaa agcgtttcac taaccacgcc gagcagagaa taccgctcat cgaaccgatc
    10921 gacagaccaa agagaacacc gcccatttca gcgatcgaga cagagagaat atcgcggata
    10981 gcaggcgtac gggttgccca ggacgccatt aacaggcccg gcaaaaagaa gaacataaac
    11041 agcgcccagg ttcggcgttt caatgcatta cgtgaagaat ttacggtcat agatcacgtc
    11101 aaaataagaa gagggaagac aacattagca aggttgtgta catttgtaca caattgcaga
    11161 cagaggaaat gacatgcgtc gcgctaacga tccgcaacgg cgagaaaaaa ttatccaggc
    11221 cacgctggag gcggtgaaac tttacggaat acatgctgtt acgcaccgca aaattgctac
    11281 ccttgccggg gtaccgttgg gatcgatgac ctactatttt tcaggaattg atgagttgtt
    11341 gctggaggcg ttcagcagtt ttactgagat catgtcccgg caatatcagg cattttttag
    11401 cgatgttagt gatgctcaag gcgcatgcca ggctatcacc gatatgatct acagctcaca
    11461 ggttgcaacg ccggataaca tggagctgat gtaccagctc tacgcgctgg ctagccgaaa
    11521 accgctatta aaaacggtaa tgcaaaactg gatgcagcgc agtcagcaaa cgctcgaaca
    11581 atggtttgaa cccggaaccg cccgcgcgct tgatgcgttt attgagggga tgacgctgca
    11641 ttttgtcacc gaccgtaagc cgctatcgcg cgaggagatt ttgaggatgg ttgagagggt
    11701 tgcagggtag tagataaatt tcaggcaaca aaaaacccat caaccttgaa ccgaaatggc
    11761 ggggttgatg ggctccacaa aatggggaca tcaaagaaaa gcagtggcac taattaagac
    11821 tgatgccctg cggaaaagtt ctgcggttgt gcaaaaaaat ttcattttca gggcaacttc
    11881 agttttatcc taatcctggc cataccatga cgatgattgt ccctgccagc gtcagcagga
    11941 cgttggcgat tgcgtaggtg cccgcatagc ccagcgccgg gatgttactg cgagctgtat
    12001 cactgatgat ctccattgcc ggcgcgcagg tgcgtgcgcc catcattgcg ccgaacaaca
    12061 gtgcgcggtt cattcgcaat acataagcac cgaacaagaa acagataacc acgggcacaa
    12121 gactgacaat caatccggca atcaacatct gaccgccaat cgcgcccaga ccgttattaa
    12181 taccgctacc ggcgctcaga ccaacgcctg ccataaacac catcaagccg aactctttca
    12241 ccatgcttaa tgccccttgc ggaatgtaac cgaaggtcgg gtggttagca cgcataaagc
    12301 ccagcataat tccggcgaat aacaacccgg cagcgttccc catgccgaaa ctgaatgtgc
    12361 tgaactggaa ggtgatcatc ccgatcatca gcccaataac aaagaaggcg cagaatgcca
    12421 gcaggtcagt gacctggctg tgaatcgaga taaagccgat gcgatcggcg atggttttta
    12481 cgcggcgggc atcgccgctg acttgtaaaa cgtcaccttt gttaagcacg acgttgtcat
    12541 caatcggcat ctcaatctgg ctacgaatga cgcggttaag gaagcaaccg tgatcggtca
    12601 acttcagttg tgcgagacgt ttacctacag cgttatggtt tttaacgacc acttcttcag
    12661 tgacgatacg catgtcgaga aggtcacgat cgaaaacttc tttaccgtta cggaagctgg
    12721 gatcgagtcg ggcatgggcg tcgggatagc ctaccaacgc tatttcatcg cccatttgta
    12781 gcacggcatc accgtctgga tttgccagaa tcccgttacg tcgaatacgt tcaatgtagc
    12841 agccggtttg tcgataaata cccagttcac gcagattttt gccgtcggtc caggccacca
    12901 gctccgggcc gacgcgatag gcgcggatca ccggtaaata aaccttacgg ttggcatcag
    12961 tgtccaggcc acgttcgcgg gcgatttgct gggcgctggt ctgtaagtcc tgatgctgca
    13021 atttcggcaa gtaacgcgca ccaacaatca aactcaccag accgattaaa taggttaagg
    13081 catacccgag gctcagatta tccagtgcca gtgagagctg cctgctttcc ataccggaat
    13141 gacgcagtgt atcgccagca ccgaccagaa ccggtgtcga cgtcatagag cctgctaaca
    13201 taccggccgt caggccaata tcccagccaa acagcttacc taaccctaag gcgatcacca
    13261 gcgcactgcc aaccatcacc agtgctaaca ttaggtaatt tttcccatcg cgaaaaaaaa
    13321 tggaaaaaaa gttcggtccg gcttcgaccc cgacgcagaa aataaacagc ataaagccaa
    13381 gattaagcgc atcggtgtta atgctgaaat gttgttggcc taataacagc gatacgacta
    13441 aaacgccaat ggaattaccc agttggatcg aaccaagtcg taactttccg agacatagcc
    13501 caagcgcgag gaccacaaat aataacagaa tgtaattccc atttaacaat tcggcgacgt
    13561 ttatattcac ggaggctaac ttcttgttta ctagtaagct gttgaaagaa atggtaattt
    13621 acgataatgt tttttaccag aattcagggc gcagattcat tcagcgcacc taaacgatag
    13681 taaagtaaca atatatttta ctagtgtaat cacattaggt atcaacggct atatgaattg
    13741 cgttggccta tattagcatg gaatgcgaag cggctttatc ttactgaacg ccacactggc
    13801 gaaaaatgtg ttcgatagac gcagtgtcag gaggaacgag tgaaacataa acaacgttgg
    13861 gcgggggcaa tctgctgttt tgtcctcttc attgtggtgt gcctttttct ggcgacgcac
    13921 atgaaaggcg cttttcgggc tgccgggcat cctgaaatcg gcttgctgtt tttcattctt
    13981 cctggagcag ttgccagttt cttttcacag cgtagagaag tcctgaaacc tctatttggc
    14041 gcaatgctgg cggcaccctg ttcaatgctt attatgcggc tgtttttttc accgacccgc
    14101 tcattctggc aagagctggc atggttacta agcgcggtgt tctggtgtgc gctgggggca
    14161 ctgtgtttct tatttatcag tagcttgttt aaaccacagc acagaaaaaa tcagtaaagc
    14221 cctcaacgcg agggcttgtc agacgatcag gcgtccagat tctctttcac ccatgcagca
    14281 aaatcggtat agccgccgat atgttgctga tcgacaaaaa tctgcggcac ggtttctacg
    14341 ggtttacctg ccttttgttg tagatcttct ttagtgatcc cttccgcacg aatatctaca
    14401 tactgatact gaaaatcatc gcgttcattg ctcaatttct cagccagatc ttttgcacgc
    14461 acacagtaag ggcaacccgg acgaccaaaa ataacggttt gcattatttc tctcctcata
    14521 gatttatgcc tgtaatgatc acgctaaaat gtattcgctg aaagtaggtt taacctgttg
    14581 cattaattgc taaaagctat aactgttaaa cacaatacag tgaaaagttt tagactgaag
    14641 gttcactttg cagagggaag cgtatgcgcg cgatcggtaa attgcctaaa agcgtgttga
    14701 tactggaatt tatcggaatg atgttgctgg cggtggcgct gctgtcggta agcgactccc
    14761 tgtcgctgcc tgagccattt tctcggccag aagtgcagat tctgatgatt tttctcggtg
    14821 ttttgctcat gcttcccgct gcggtggtgg ttattcttca ggtggcaaaa cgtcttgccc
    14881 cacagctgat gaaccgtcca ccgcagtatt cacgttcaga aagagaaaaa gataatgacg
    14941 ccaaccattg aacttacttg tggccatcgc tccattcgcc atttcactga tgaacccatt
    15001 tccgaggcgc agcgtgaggc gattattaac agcgcccgtg cgacgtccag ttccagtttt
    15061 ttgcagtgca gtagcattat tcgcattacc gacaaagcgt tacgtgaaga actggtgacg
    15121 ctgaccggcg ggcaaaaaca cgtagcgcaa gcggcggagt tctgggtgtt ctgtgccgac
    15181 tttaaccgcc atttacagat ctgtccggat gctcagcttg gcctggcgga acaattgctg
    15241 ctcggtgtcg ttgatacggc aatgatggcg cagaatgcat taaccgcagc ggaatcgctg
    15301 ggattgggcg gggtatatat cggcggcctg cgcaataata ttgaagcggt gacgaaactg
    15361 ctgaaattac cgcagcatgt tctgccgctg tttgggctgt gccttggctg gcctgcggat
    15421 aatccggatc ttaagccgcg tttaccgtcc tccattttgg tgcatgaaaa cagctatcaa
    15481 ccgctggata aagacgcact ggcgcagtat gacgaacaac tggctgaata ttacctcacc
    15541 cgtggcagca ataatcgccg ggatacctgg agcgatcata tccgccgaac aatcattaaa
    15601 gaaagccgcc catttattct ggattatttg cacaaacagg gttgggcgac gcgctaaaac
    15661 cgccacgccg atgtatgata cgcgggcttt tgaccaggtc tgacagagag gtgcagggtg
    15721 aaaattgcca tattgtcccg ggatggaacg ctctattcgt gtaagcggct gcgtgaagcc
    15781 gctatacagc gcggtcacct ggttgaaatt cttgatccgc tttcttgcta catgaacata
    15841 aatcctgcgg cgtcttctat tcactacaaa ggccgcaagt taccccattt tgacgcagtg
    15901 atcccgcgta ttggcaccgc cattaccttt tatgggacgg cggcactgcg ccagttcgag
    15961 atgctgggga gctatccgct caatgagtcg gtcgccattg cccgggcgcg tgacaaattg
    16021 cgttccatgc aactgctggc gcgtcagggc atcgacctgc ctgtcacggg cattgcgcat
    16081 tcgccggatg ataccagcga tttaatcgac atggtcggtg gtgcgccgct ggtggtcaag
    16141 ttggttgaag gcacgcaggg aattggcgtc gtgctggcgg agacgcgtca ggcggcggaa
    16201 agcgtgattg acgctttccg cggtctgaac gcgcatattc tggtgcagga atatatcaaa
    16261 gaggcgcaag ggtgcgatat ccgctgtctg gttgttggcg atgaagtggt cgctgcgatt
    16321 gaacggcggg cgaaagaggg cgattttcgt tccaatttgc atcgtggcgg cgcggcaagc
    16381 gtcgccagta tcacaccaca ggagcgtgaa atcgcgataa aagccgcgcg aacgatggcg
    16441 ctggacgttg ctggtgtgga tattctgcgt gctaatcgcg ggccgttggt gatggaagtg
    16501 aatgcgtcgc cggggctgga aggaatagaa aaaaccaccg gtatcgacat cgcgggtaaa
    16561 atgatccgct ggatcgaacg ccacgctacg acagaatatt gcctgaaaac gggtggttag
    16621 tcgcaatcac attactgatc atggttttgc ctgctctttt tgcgtaagct gtgccggtct
    16681 ttttatcgaa agaggttgta caaaattatg acatcgctgg tcgttcctgg tctggatacg
    16741 ctgcgtcaat ggctcgatga cctggggatg agtttttttg aatgtgataa ctgtcaggct
    16801 ctgcatctgc cccatatgca gaatttcgac ggtgtctttg atgccaaaat cgatctgatc
    16861 gataacacga tcctgttttc tgccatggcg gaagtccgac cttcagccgt attgccgctg
    16921 gcggcggatt tatctgccat caatgccagt tcgctgaccg tgaaagcatt tcttgatatg
    16981 caggatgata atctgccaaa gctggtggtt tgccagtctt tatccgttat gcagggcgta
    17041 acctatgagc agtttgcatg gttcgtgcgt cagagcgaag agcagatttc gatggtcatt
    17101 cttgaagcta atgcccatca actgctgtta ccgactgacg atgaagggca aaacaacgtt
    17161 accgaaaact atttcctcca ctgataactc ctttcgagca cgcagtcgct ggtgcagtgg
    17221 ctgcgcgctg caaaattatc tgctgttttt aaccttttct taaagattat ttcacttctc
    17281 ttgtgtcgat ttggctttat cacatagagc aaatacgcat aaaaatttgt taaataccgt
    17341 tttttaatcc gagctatagt ctcaaaccct ggctaaagtt attcttgcga tgcttttata
    17401 tagcgagcag tgctggccgg gagagagttc tcttttctta caccgcgccg ataaaaaata
    17461 tgcacgttta ttgcatatct ttcagtgtga caacttttgt tcgtttgtta acgaactttc
    17521 agaaggaaag agatatgacc gccttaaata aaaaatggct ttcgggtctg gttgcgggtg
    17581 ctctgatggc cgtctctgtc ggcacgctcg cggctgaaca aaaaacactc cacatttata
    17641 actggtctga ttatatcgcc cccgacacgg tggctaactt tgaaaaagaa accggtatta
    17701 aagtcgtcta cgatgttttc gactctaacg aagtactgga aggcaaatta atggccggga
    17761 gcaccggctt tgacctggtg gtgccgtcag caagctttct tgaacgccag ctgacagcgg
    17821 gagttttcca gccgctggat aaaagcaaat tgccggagtg gaagaatctc gacccggaac
    17881 tgctgaagtt ggtcgccaaa cacgatcccg acaataaatt tgctatgccc tatatgtggg
    17941 cgacgaccgg gattggctat aacgtcgata aagtcaaagc ggtgctgggc gaaaacgcgc
    18001 ccgtcgatag ctgggacttg atcctcaagc ctgaaaatct ggaaaaactg aaaagctgcg
    18061 gtgtatcttt cctcgatgcg ccagaagaag tttttgctac cgtgctgaat tatctcggca
    18121 aagatcccaa cagcactaaa gcggatgatt acaccggacc ggctaccgat ctgctgttaa
    18181 agctgcgccc gaacattcgt tatttccatt catctcaata tattaacgac ctggcaaacg
    18241 gcgatatttg cgtcgctatc ggctgggcag gtgatgtctg gcaggcgtca aaccgcgcga
    18301 aggaagcgaa gaatggcgtg aatgtctcgt tctcgattcc aaaagaaggg gcgatggcgt
    18361 tctttgatgt attcgccatg cctgcggatg ccaaaaataa agacgaagcc tatcagttcc
    18421 tgaattacct gctgcgtcct gatgtggtgg cacatatctc tgaccatgtg ttctatgcta
    18481 acgccaataa agcagccacg ccgctggtga gtgcggaagt ccgtgataac ccgggtattt
    18541 atccgcctgc ggatgtccgc gcgaagttgt tcactctgaa agtacaggct ccgaaaatcg
    18601 accgtgtgcg cacccgcgcg tggaccaaag tgaagagcgg aaaataatcc gcagccttag
    18661 atgccgggcg ggagcgccac tccctccggc aattcgcacc aacatggtgc gcttgcacac
    18721 atttaatgcc ggagagcagc cgtgaatgac gctatccctc gcccccaggc gaaaacccgt
    18781 aaggcgctga ccccgctatt agaaatccgc aatctgacca aatcctacga tggtcagcat
    18841 gcggttgatg atgtcagcct gaccatctac aaaggtgaaa tctttgcgct gctgggcgca
    18901 tccggctgtg gcaagtccac gctgctgcgt atgctggcag gtttcgaaca accttcagcc
    18961 gggcagatta tgcttgatgg cgtcgatttg tcacaggtgc cgccttacct gcgccccatc
    19021 aatatgatgt ttcagtctta cgcgctgttt ccgcatatga ccgtggaaca gaacatcgct
    19081 tttggcctga aacaggacaa actaccgaaa gcggaaattg ccagccgggt caatgagatg
    19141 ctcggccttg tgcatatgca ggagttcgcc aaacgcaaac cgcatcagct ttccggtggt
    19201 cagcgacagc gtgtggcact ggcccgaagc cttgccaagc gcccgaaact attactgctc
    19261 gacgagccga tgggcgcgct ggataaaaag ctgcgtgacc ggatgcagct tgaagtggtg
    19321 gatattctgg aacgcgtcgg tgtgacttgt gtgatggtca cccacgatca ggaagaggcg
    19381 atgaccatgg cggggcgtat cgccattatg aatcgtggga aatttgtcca gattggcgaa
    19441 ccggaagaga tctacgagca tccgactacc cgctatagcg ccgaatttat tggctcggtt
    19501 aatgtctttg aaggtgtact caaagagcgt caggaagatg gtctggtgct ttattcgccg
    19561 gggctggtgc atccactgaa agtcgatgcg gatgcttcag tggtcgataa cgtgccggta
    19621 cacgtagcgc tgcgtccgga aaaaatcatg ctttgcgaag agccgcccgc caatggttgt
    19681 aacttcgcgg tgggggaggt gatacacatt gcctatctcg gcgatctttc ggtgtatcac
    19741 gttcgtctga aaagtgggca gatgattagc gcccagctac aaaacgccca tcgtcatcgt
    19801 aaagggttac cgacctgggg cgacgaagtg cgtttgtgct gggaagtgga cagctgtgtg
    19861 gtgctgacgg tttaaggagc aaagatgagt acacttgaac ctgctgccca gtcgaaaccg
    19921 ccgggcggat ttaagctgtg gttgtcgcag ttgcaaatga agcatgggcg caaactggtc
    19981 attgcgttgc cgtatatctg gttgatcttg ctgtttctgc tgccatttct gattgtcttt
    20041 aaaataagcc tggcggagat ggcgcgcgct attccacctt ataccgaact gatggagtgg
    20101 gctgacgggc aactgtccat cacccttaat ttcggtaatt tcctgcaact gaccggcgat
    20161 ccgctctatt tcgatgctta tctccagtcg ttacaggtgg cggcgatttc gacattttgc
    20221 tgtttactga tcggctatcc gctggcgtgg gcggtggcgc acagtaagcc ttcgacccgt
    20281 aatatcttat tactactggt gatcctgccg tcgtggacct cgtttctgat tcgtgtctac
    20341 gcctggatgg gaatattaaa aaacaacggt gtgctgaata attttctgct gtggctgggg
    20401 gttatcgatc aaccgctgac cattcttcat accaatctgg cagtttatat tggcattgtt
    20461 tacgcctacg tgccgtttat ggtgctgccg atttataccg cgttgattcg tattgattat
    20521 tcgctggtgg aagcagcgct ggatctcggt gcacgaccgc tgaaaacgtt ctttactgtg
    20581 atcgtgccgc tgactaaagg tgggattatt gccggatcga tgctggtgtt tatcccggct
    20641 gtgggcgagt ttgtgatccc ggaactgctc ggtggcccgg acagcatcat gatcgggcgc
    20701 gtgctatggc aagagttttt caataaccgc gactggccgg tggcctcggc ggtagcgatc
    20761 atcatgttgc tgctgctaat tgtgccgata atgtggtttc acaaacacca gcaaaaaagc
    20821 gtgggagaac acggatgaat aatttaccgg tagttcgttc gccctggcgg attgtgattt
    20881 tgctgttggg cttcaccttt ctctacgcgc caatgctgat gctggttatc tattcgttta
    20941 acagctcgaa gctggtgacg gtgtgggccg gctggtcaac gcgctggtat ggcgaattat
    21001 tgcgcgatga cgcgatgatg agtgcggttg gtttaagtct gacaattgcg gcctgtgcgg
    21061 cgacggcggc ggcgatcctc ggaactattg cggcggtggt gctggtgctc tttggcaggt
    21121 ttcgcggatc aaatggcttt gcctttatga tcaccgcgcc gctggtaatg ccagatgtca
    21181 tcacgggctt gtcgctgttg ttattattcg tcgcgcttgc tcatgccatt ggctggcctg
    21241 cggaccgcgg tatgcttacc atctggctgg cgcatgtcac tttctgtacg gcttatgtgg
    21301 cagtcgttat ttcgtcgcgt cttcgggaac tggatcgctc gatagaagaa gcagcgatgg
    21361 atctcggtgc gacgccgctg aaagtgtttt tcgtcattac gctaccgatg atcatgcccg
    21421 cggtcatttc tggctggtta ctggctttta ctttgtcgct tgatgatctg gtgatcgcca
    21481 gctttgtatc cgggccggga gccaccacgt taccgatgct ggtcttttcc agcgtgcgga
    21541 tgggggtgaa tccggaaatc aacgccctag caacattgat cctcggcgcg gtcggaattg
    21601 tcggatttat cgcctggtat ctgatggctc gcgcagaaaa acagcggata cgcgacatcc
    21661 agtgtgcaag acgtggctga agacactaaa atttgccaac ctggctacat aatgccgcgt
    21721 atgtcgcggc attgttttca tggaagacga aacgttggga ttttttaaga aaacatcttc
    21781 atctcatgct cgcctgaatg tgcctgcgct tgtgcaggtg gcggcgctcg ccattattat
    21841 gatccgtggc ctcgacgtgc tgatgatttt caatacgctg ggcgtgcgcg gtattggcga
    21901 gttcattcat cgcagcgtac aaacctggag tttaacgctg gtctttttaa gcagtctggt
    21961 gctggttttc attgagatct ggtgtgcgtt ttcactggtg aaagggcgtc gctgggcgcg
    22021 ctggctgtat ctgctgacac aaatcactgc cgcaagttac ttgtgggcgg cttcgctggg
    22081 gtacggttat ccggagctgt tcagcattcc cggtgaatca aaacgtgaaa tcttccatag
    22141 cctgatgctg cagaagctgc cggatatgct catcctgatg ctgctgttcg ttccctcgac
    22201 cagtcggcgg ttcttccagt tgcaataatg tgtataatcg tcgcccctga tgatgtgaag
    22261 gtcaatgtat gcagtgcgca ctttacgacg cgggtcgctg tcgttcctgt cagtggataa
    22321 cgcagccgat tccagagcaa ctctccgcta aaaccgccga tcttaaaaat ctgctcgccg
    22381 actttccggt tgaggaatgg tgcgcgccgg tgtcaggccc ggaacaaggg tttcgtaata
    22441 aagccaaaat ggtggtgagt ggtagcgttg aaaaaccact gctcggtatg ctgcatcgcg
    22501 acggcacacc ggaagacctt tgtgactgcc cgctttatcc tgcctcattt gcgcccgttt
    22561 ttgcggcgct aaaaccgttt atcgcccgag cggggttaac gccctacaac gtggcgcgta
    22621 agcgtggcga actgaaatac attctgctga ctgaaagcca gagcgatggc ggcatgatgc
    22681 tgcgctttgt actgcgttct gaaaccaaac tagcacaact gcgtaaggca ttgccgtggt
    22741 tacaggcaca attgccgcag ctaaaagtta tcaccgtcaa tattcagccg gtacatatgg
    22801 cgattatgga aggggagacg gagatctacc tgaccgaaca acaggcgctg gtagagcgtt
    22861 ttaacgatgt gccgctgtgg atccgtccgc aaagtttctt ccagactaat ccggcggtcg
    22921 ccagccagct gtacgctacc gcgcgcgact gggtacgaca gctgccggtt aaacatatgt
    22981 gggatctgtt ctgcggtgtg gggggctttg gtttacactg cgcgacgcct gacatgcagt
    23041 taaccgggat cgaaattgcg ctagaggcca ttgcctgtgc aaagcagtca gccgctgaac
    23101 tgggcttaac gcgtctgcaa tttcaggcgc tggactccac tcagtttgcc accgctcagg
    23161 gggaggtgcc ggagctggtg ctggttaacc cgccgcgccg cggcattggt aaaccgctgt
    23221 gtgattatct ctcaacgatg gcaccgcgtt ttattatcta ctccagctgt aacgcccaaa
    23281 ccatggcgaa agatatccgc gaactgccag gttaccgcat cgaacgggta cagctttttg
    23341 atatgttccc gcacaccgcg cactatgaag tgctgacgct gctggtaaaa atgtaaatac
    23401 atcattcaaa tcaatatcac ctcggcatac atccatccaa aataataatc gtgatcgatc
    23461 gattaataat aaccgatcga tcacggttat atattgctct atttttgcaa atcaaatcac
    23521 aaataatcaa ataagatctt taattgaact gttttgatta ctacagtgca cttaccgaat
    23581 acatacagag gcattgctta gcgaaacaca aaatttcctg agacattatt aacgtcagca
    23641 gaaggttcat gcattgacaa cggaaatgta cgcgtatgga gggcgttccg acgatgtttg
    23701 ccaaattcat agatgttatt caaacgtttt taactgaacc ggcaatttta attggtttgc
    23761 tggtgggtat tggttatgcc cttgataaga aatcgccaat aaaaattatt accggcatgg
    23821 tcagcgccat ggtgggatta atgatggtgt tatttggtgg tttccagttt tccgcaacat
    23881 ttaaaccggt agcagaagcc gtaagtaaag cctatggtgt tcacggatat ttaatggatt
    23941 cctacgcaat gaaagcggca acgcaaattg cattggggga taattttggt tatgtcgggt
    24001 atgtatttgt actggcgttt tttaccaacc tgctgctggt attatttggt cggtatactg
    24061 gtgccaaagg gatatttttg acagggaata ccggtgtttc acattctcag gcggtattat
    24121 ggttgattgt cttttggctg ggttttagct ggactacatc cattattatt gccggaatat
    24181 taacgggtgt attctgggcg ttctccacca cgctcattgt taagcccatt gccaaagtta
    24241 ccaaagatgc cggttttacc attgctcata accagatgtt gggattgtgg tttttctcca
    24301 aattcgccca taaatttggt gaccctgaaa agcacgatgc tgaaaacctg aaacttcccg
    24361 ggtggctggc gatttttaac cataacgtaa cggctatcgc cattgtgatg acgctgtttg
    24421 tcggtggttt cttgctatca acgggtatcg ataatgtcca gttaatggcg aaaggcaagc
    24481 cctggtacat ctatatcatc aaccttggct tacagttctc catgtatatg gtcattctgc
    24541 tgcagggtgt gcgcatgatg gtgggcgaaa ttaacggttc gtttaaaggc tggcaagatc
    24601 gctttattcc taacgctatt cctgctgtcg atgttgcagc gctgttacct ttttcaccca
    24661 atgccgcaac gttaggcttc gtcttctgta cttttggcac cattttttcg atggggatct
    24721 tattactggt tcacagccca attatggtat tgcctggctt tgtacctctg tttttctctg
    24781 gcggtccaat tggcgtatta gcaaaccgca tgggcggata ccgttccgta attatctgta
    24841 cgttcttgct gggtattatt cagaccttcg gtacggtgtg ggctattccg ttaaccgggc
    24901 ttgctgaaaa tggcgtcggc tggacaggaa tatttgactg ggcaaccgta tggcctgcta
    24961 tttgtgaagt tctgaaattt atcgctgcaa cattccatct tggtccttac gcgggttaat
    25021 taaatattaa gggtttaaaa atgaaagata caattaatat tctgtttgtc tgtggttacg
    25081 gtgtaggcag tagcgttatg ttacaaacgg tcgttaaaaa agcgctgacc aaatatgatt
    25141 tcaaagttga aatggaacat accgccgcag gtgaagtcgg cggatttacc gactgggcgg
    25201 atatttttgc tatttccaaa aaattactcg atgtggttag tcttgacccg aaacaagggc
    25261 aatatttgat tccgatagag aatattatgg acggtgaaac tattggtcag caaatttatg
    25321 aagtggttca gcaacatttt ccgcatttaa ttaatcaata aggtagggcg taatgagagc
    25381 gattattctg ctgttcgata gtctgaataa acgttatctg ccaccttatg gcgatgcgtt
    25441 aaccaaagcg cctaatttcc aacgtctggc ggctcatgcc gccacctttg aaaacagtta
    25501 cgtcggcagt atgccgtgta tgcctgcccg gcgggagttg cataccgggc gatgtaactt
    25561 cctgcatcgg gagtgggggc cgttagaacc gtttgacgat tccatgccgg agctgcttaa
    25621 aaaggcgggg atctataccc acctgataag cgatcatttg cattactggg aagatggcgg
    25681 cgggaattat cataatcgct acagttcgtg ggagattgtg cgcggacagg aaggcgatca
    25741 ctggcatgca agcgttgcgc aaccgcctat tcccgaggtg ctacgggtgc cgcaaaagca
    25801 gaccggtggc ggtgtttctg gtctgtggcg ccatgactgg gcaaaccggg aatatattca
    25861 gcaggaagcg gattttccac aaactaaagt gtttgatgca ggctgtgcct ttatccacaa
    25921 aaatcacgcg gaagataact ggttattgca gatcgaaacg tttgatccgc acgagccgtt
    25981 tcacacaacg gaagagtatc tttccttgta tgaagataac tgggatggac cgcattatga
    26041 ctggccgcgt ggccgggtgc aggaaagcga cgaggccgtg gagcacatcc gttgccggta
    26101 tcgttcgctg gtgtcgatgt gcgatcgcaa cctggggcgc attctcgacc tgatggatga
    26161 gcacgatctg tggcgagata ccatgctgat tgtcggcact gaccacggct ttttactcgg
    26221 tgagcatggc tggtgggcga aaaaccagat gccttattac aacgaagttg ccaataatcc
    26281 gttgtttatc tggaacccgc gcagtggtgt aaaaggagag cggcgacagg cactggtaca
    26341 aatgatcgac tgggcgccaa cgttgtatga cttttttcaa cagccagtgc cgcccgatgt
    26401 gcaggggcaa ccgctggcga aaacggtcag tcacgatgaa ccagtacgca gctcggcgat
    26461 gtttggtgtt ttcagtggtc atgctaacgt aactgatggg cgttatgtgt atatgcgtgc
    26521 agcgctgccg gggcgtgagg atgatattgc caactacacg ttgatgtcct gcaaaatgaa
    26581 cagccgctat ccggtggatg agatgcgggc tttatcgctg gccccaccgt ttcgttttac
    26641 caaagggtta caggtattac gcatcccggc acaggaaaaa tataaggggt tgaatcagtt
    26701 tggtcatttg ctgtttgatc tgcaaaacga tccgcagcag ctacatccga ttcatgatga
    26761 tgtgatcgag tcccggatga tcgcgttgct gattcagttg atgaaagata atgatgcgcc
    26821 gccagagcag tttcagcgcc tgggattagc gtagagaagt gattttgccg gagtgggtgt
    26881 tcgcccactc cggcaaatga ataattattg cgggaaccac tgatcgctga tttgttgata
    26941 tgtaccgtca gctttaattg ctgccagcgc gttattcagt ttttccagca gagctttgtt
    27001 atccggacgt acagcgatac ccagaccggt gccaaagtat tgcggatcgg tcactttttc
    27061 agtagcaacg cccagttgcg gattggtttt cagccattca ttaaccaccg cagtgtcacc
    27121 aaatacgcca tcaatacggc tatttttcag atcgataaag gcattttgat aactgtcata
    27181 ggagaccgtt ttcacttccg ggtgctgatc ctgaatatat ttctggtgcg tagtaccgtt
    27241 ttccatccca atgcgtttgc ctttcaggtc ggcaaacgtt ttgtaggtat cttttttggc
    27301 aatcacgacg gctgagtttt cgtaataggg cgtggtgaac gatacctgct tgctacgatc
    27361 tggtgtgata tccataccgg agattacggc gtcatatttc ctgaatttca gtgacgggat
    27421 caggctgtcg aacgcgtgat tggtaaaagt acattctgcc tgcatctgtt tgcataacgc
    27481 ttttgctagg tcgatatcaa agccgacgat ctggttattg gcatccatcg actcgaacgg
    27541 cggataggtc gctgatacgc caaaattgat tttctctgcg gcagaagcgc cgaacgcaca
    27601 ggatgccagt aatgcggcaa gaactaactt tttcatgatg gaactcccgt ctgtcaatct
    27661 tatgattatt ggccgtgtct gcggcatggg ataacaatgc catcaagtga a
//
LOCUS       DAZFAB010000054        27384 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZFAB010000054 DAZFAB010000000
VERSION     DAZFAB010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27384
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.054"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(83..364)
                     /locus_tag="ACHJWA_002672"
     CDS             complement(83..364)
                     /locus_tag="ACHJWA_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2574 family protein"
                     /protein_id="HFO4309246.1"
                     /translation="MNKYWLSGAVFLAYGLASPAFSSDTTTLTINGRISSPTCSMDVV
                     NNHLQQRCGQLLQRVETNYRASSTAKGVTTEVVAVGNNDKRKIVLNRYD"
     gene            complement(626..1735)
                     /gene="apbC"
                     /locus_tag="ACHJWA_002673"
     CDS             complement(626..1735)
                     /gene="apbC"
                     /locus_tag="ACHJWA_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFO4309247.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1867..3900
                     /gene="metG"
                     /locus_tag="ACHJWA_002674"
     CDS             1867..3900
                     /gene="metG"
                     /locus_tag="ACHJWA_002674"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFO4309248.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYTRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            4041..4769
                     /locus_tag="ACHJWA_002675"
     CDS             4041..4769
                     /locus_tag="ACHJWA_002675"
                     /inference="COORDINATES: protein motif:HMM:NF017243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HFO4309249.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHINWGKVGTNGQSQIKSFAD
                     VAAAEKAKLKLIAEKTKKGYVENASANVHIPPITKATPEVETSPESKNQRPWLADDAV
                     IPVTDDINRFAFPHRSRPREINYLRKDGEIWKRIADNTRAYDPDNNYRSYPENWQQAF
                     AELQRRILDNQQTGSAQSDAALLWSFWNSYSADELVDDLVIRCGLESAVEIVLFALQL
                     KYKPVKGAVTTIIPPNHEAESLPS"
     gene            6726..7850
                     /locus_tag="ACHJWA_002676"
                     /pseudo
     CDS             6726..7850
                     /locus_tag="ACHJWA_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            7863..10848
                     /locus_tag="ACHJWA_002677"
                     /pseudo
     CDS             7863..10848
                     /locus_tag="ACHJWA_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            10858..14487
                     /gene="yehI"
                     /locus_tag="ACHJWA_002678"
     CDS             10858..14487
                     /gene="yehI"
                     /locus_tag="ACHJWA_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
                     /protein_id="HFO4309250.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRRWPGEPVSVITGSLIQ
                     TLGDELLQQAGQKENITWNYDKCSLEWQSAIQQAINLTGEHKPSIPALTMAALICIAQ
                     NDSQQLLDEIVQQEGLEYATDVVISRQCIARRYESDSLVVTLQYQDEDYGYGYGSATY
                     NDFDLRLRKHLSLAEESCWQRCADKLIAALPGIPKIRRPFIALILPEKPEIANELASL
                     ESSRSSLHSKEWLKVVATDNTAVKKLERYWGLDVFSDREASYMSQENRFGYAACASLL
                     REQGLAAVPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPARPGYPIIEDKKLPAQQKAREEYWRTLLQTLMASQPQLAAE
                     VMSWLSTQARAVLKSYLSALPKPVIDGTDNSNLPEILVSPPWRSKKKMTAPRLDLARL
                     ELTPQVYWQPGERERLAATESARYFSTESLAQRMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPEAQLAEQALRSGHIEALINIWEND
                     NYSRYRPEKSVWNLYLLAQLPREMALTFWLRINEKKHLFAGEDYFLSILGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTATALIPL
                     VFTKSSDKSEAALLALRLLYEHGHGELLQTVANRWQRTDVWPALEHLLKQGPMEIYPA
                     RIPKAPDFWHPQMWSRPRLITNNQPVTDDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQGKSARA
                     VSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRLV
                     PTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPEA
                     LARLKGLKKDATQVSKNLLPRLEAALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWGV
                     YPANEPRRLLNAFRVAAEGGFCNAQDEPIDLPADALIGIAHPLEMTAEMRSEFAQLFA
                     DYEIMPPFRQLARRTVLLTPDESTSNSLTRWEGKSATVGQLMGMRYKGWESGYEDAFV
                     YDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVYRDNKSVTFAELDVFNLSEALSAP
                     DVIFH"
     gene            14549..14866
                     /gene="yehK"
                     /locus_tag="ACHJWA_002679"
     CDS             14549..14866
                     /gene="yehK"
                     /locus_tag="ACHJWA_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YehK"
                     /protein_id="HFO4309251.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRSTFHQRDREDADPLINDALIYIRDECIDERKLRGESPETVIAIFNRELQNIFNQ
                     EIE"
     gene            16107..17195
                     /gene="yehL"
                     /locus_tag="ACHJWA_002680"
     CDS             16107..17195
                     /gene="yehL"
                     /locus_tag="ACHJWA_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFO4309252.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            17206..19485
                     /gene="yehM"
                     /locus_tag="ACHJWA_002681"
                     /pseudo
     CDS             17206..19485
                     /gene="yehM"
                     /locus_tag="ACHJWA_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
     gene            19478..20614
                     /gene="yehP"
                     /locus_tag="ACHJWA_002682"
     CDS             19478..20614
                     /gene="yehP"
                     /locus_tag="ACHJWA_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO4309253.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSSIPLARDFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRHSEQWQLV
                     LLVDLSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDMAQALVNVGAQIAAMTPGELATWLAENLQS"
     gene            20611..22614
                     /gene="yehQ"
                     /locus_tag="ACHJWA_002683"
     CDS             20611..22614
                     /gene="yehQ"
                     /locus_tag="ACHJWA_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFO4309254.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANGQALKEAQCTCGASGMCRHRVMLVLSYQRLCATTRPTEKEE
                     EWQPAIWLEELATLPDATRKRAQVLVAKGITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVEAKTQQAEFTHLIWQMRSEHVTSSDDPFASEEG
                     NACRQYVQQLSQALWLGGISQPLIHYEAAFSRAQQAAERCNWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWRMLSAPLRQPGIVALR
                     EYLRQRPPSCIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAVERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVASLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAIGQAELLANDLTA
                     VGFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPLLQQQG"
     gene            22739..23200
                     /gene="yehR"
                     /locus_tag="ACHJWA_002684"
     CDS             22739..23200
                     /gene="yehR"
                     /locus_tag="ACHJWA_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFO4309255.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(23242..23712)
                     /gene="yehS"
                     /locus_tag="ACHJWA_002685"
     CDS             complement(23242..23712)
                     /gene="yehS"
                     /locus_tag="ACHJWA_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFO4309256.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(23759..24478)
                     /gene="btsR"
                     /locus_tag="ACHJWA_002686"
                     /gene_synonym="yehT"
     CDS             complement(23759..24478)
                     /gene="btsR"
                     /locus_tag="ACHJWA_002686"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFO4309257.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(24475..26160)
                     /gene="btsS"
                     /locus_tag="ACHJWA_002687"
     CDS             complement(24475..26160)
                     /gene="btsS"
                     /locus_tag="ACHJWA_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFO4309258.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYLELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISVRREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            26382..26447
                     /locus_tag="ACHJWA_002688"
                     /pseudo
     CDS             26382..26447
                     /locus_tag="ACHJWA_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            26675..26923
                     /locus_tag="ACHJWA_002689"
     CDS             26675..26923
                     /locus_tag="ACHJWA_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO4309259.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWKSADEWFVH"
     gene            complement(26920..27054)
                     /locus_tag="ACHJWA_002690"
     CDS             complement(26920..27054)
                     /locus_tag="ACHJWA_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HFO4309260.1"
                     /translation="MKLILGTACKTRHLVCKNVLRLVGELSIVLVLDDFQPLPILRAN
                     "
     gene            complement(27085..>27384)
                     /locus_tag="ACHJWA_002691"
     CDS             complement(27085..>27384)
                     /locus_tag="ACHJWA_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFO4309261.1"
                     /translation="EELLDKIEVCAFNVEHAQLQVPESLRTCPVTLCEPEDGVFMRNS
                     MNSNVCMLYDKMALIHLVKTRAAHPLSRESIAVSMIVGRDNCAFDPDRGNFVLKN"
BASE COUNT         7086 a         6953 c         6726 g         6619 t
ORIGIN      
        1 tttatgggat gaataaaaaa gccaaaatca ttcataggaa tgattttggc tttttaaata
       61 tgcttaaaac ggtgttggca aattaatcgt agcgatttaa cacgattttg cgtttgtcgt
      121 tattacccac agctaccact tctgtcgtaa cgccttttgc agtagaagaa gcacgatagt
      181 tggtttcaac tcgttgtaac aactgcccac aacgttgctg aagatgatta ttaacaacat
      241 ccatactgca tgtaggggaa ctaattctgc cattgatggt taatgttgta gtatcagaag
      301 agaacgcggg tgatgctagt ccataagcaa gaaaaacagc tcctgacaac caatacttat
      361 tcattgaacg ttatccctgt agtaaaggtt atgccgggta gattgagtgc ataacacact
      421 atagctgtac atccgctaca cagccacgag ggatgataaa gagacagtgg ttgcacaatc
      481 aatcgccttt gtacagtttc ggactaagat ccactgaata ttcgcagtat taaataagtg
      541 ttcattaatg cggcaaaaac aaatacaccg catcaatata acatctctgc ggtgtatgaa
      601 gaataaccag aatggttaat aggcgttaga ccgcgcggaa ggaaatctcg cctggaatga
      661 cttcaccctg ccagtagagc tgagctgcaa cgcggtcagc cagctggcgg tagatagcgg
      721 taaattcgct ctccggacgg ctgataaccg ttggcgtgcc tttatcgaga tcctcacgta
      781 aggagatgtg taacggcatc tgccccagca gctgtgtatg gtatttttca gccagtttct
      841 ctgcaccacc ggtaccgaaa atcggttcat ggtgaccaca gttactgcaa atatgcacac
      901 tcatgttctc gacaataccg agcaccggaa cctcgacttt ctcgaacatc acaatgcctt
      961 ttttcgcatc gatcaacgcg atgtcctgcg gggtggtgac cacgactgca ccagttactg
     1021 gaatgttttg cgccagcgtc aattgaatgt caccggtgcc tggcggcata tcaagcacca
     1081 gatagtcgag atccggccac aaggtttcct gcaacatctg cattaacgcc ttgctcgcca
     1141 tcggtccacg ccacaccatc gcattgtcgt cagtgaccag ataaccaatt gaattggttg
     1201 ccaggccatg agacataatc ggtgccatat gggtaccgtc cggcgaagtt ggacgctgat
     1261 tttctgcgcc cagcatggtg ggaattgatg ggccgtagat atcggcgtcc agaatgccga
     1321 ctttcgcccc ttccgccgcc agtgccagcg ccaggtttac cgccgtggag gatttaccca
     1381 cgccgccttt accggaactg accgcaatga tgttcttcac gccattaata cccggctgat
     1441 tcttcacgcg ttttaaggtg gcgatattgt gcgacagttt ccagtcgata gctttcgccc
     1501 cggtgatacg cagcagttcg gcgctacatt gctcttttaa ctcttcaaac gcgctatgcc
     1561 agacgaatgg cataaccagt tccacatgca acgtgtcgtc catccaggcg acatggtgca
     1621 acgctttcag cgtggtgagg ttatgcttca gggttgggtg ctgaaaattg gccagtgtcc
     1681 cggcgaccat tgcgcgcaag gcttctggtg atttggcctg ggattgttcg ttcatcccga
     1741 ctcctttttt gtatagataa accatcagct gatagtttac ctgaagaata tagagaagta
     1801 cttacttaac attttcccat ttggtactat ctaacccctt ttcactatta agaagtaatg
     1861 cctactatga ctcaagtcgc gaagaaaatt ctggtgacgt gcgcactgcc gtacgctaac
     1921 ggctcaatcc acctcggcca tatgctggag cacatccagg ctgatgtctg ggtccgttac
     1981 cagcgaatgc gcggccacga ggtcaacttc atctgcgccg acgatgccca cggtacaccg
     2041 atcatgctga aagctcagca gcttggtatc accccggagc agatgattgg cgaaatgagt
     2101 caggagcatc agactgattt cgcaggcttt aacatcagct atgacaacta tcactcgacg
     2161 cacagcgaag agaaccgcca gttgtctgag cttatctata ctcgcttgaa agaaaacggt
     2221 tttattaaaa accgcaccat ctctcagctg tacgatccgg aaaaaggcat gttcctgccg
     2281 gaccgttttg tgaaaggcac ctgcccgaaa tgtaaatccc cggatcaata cggcgataac
     2341 tgcgaagtct gcggcgcgac ctacagcccg actgaactga tcgagccgaa atcggtggtt
     2401 tctggcgcta ccccggtaat gcgtgattct gaacacttct tctttgatct gccctctttc
     2461 agcgaaatgt tgcaggcatg gacccgcagc ggtgcgttgc aggagcaggt ggcaaataaa
     2521 atgcaggagt ggtttgaatc cggtctgcaa cagtgggata tctcccgcga cgcgccgtac
     2581 ttcggttttg aaattccgaa cgcgccgggt aaatatttct acgtctggct ggatgcgccg
     2641 attggctata tgggttcttt caagaatctg tgcgacaagc gcggcgacag cgtaagcttc
     2701 gatgaatact ggaagaaaga ctccaccgcc gagctgtacc acttcatcgg taaagatatt
     2761 gtttacttcc acagtctgtt ctggccagca atgctggaag gcagcaactt ccgcaagccg
     2821 accaacctgt ttgttcatgg ctatgtgacg gtgaacggcg cgaagatgtc taagtctcgc
     2881 ggtaccttta ttaaagccag cacctggctg aatcattttg acgccgacag cctgcgttac
     2941 tactacactg cgaaactctc ttcgcgcatt gatgatatcg atctcaacct ggaagatttc
     3001 gttcagcgcg tgaatgccga tatcgttaac aaagtggtta acctggcctc tcgtaatgcg
     3061 ggctttatca acaagcgttt tgacggcgtg ctggcaagcg aactggctga cccgcagttg
     3121 tacaaaacct tcactgatgc cgctgaagtg attggtgaag cgtgggaaag ccgcgaattt
     3181 ggtaaagcag tgcgcgaaat catggcgctg gctgatctgg ctaaccgcta tgtcgatgaa
     3241 caggctccgt gggtggtggc aaaacaggaa ggccgcgatg ctgacctgca ggcgatttgc
     3301 tcaatgggca tcaacctgtt ccgcgtgctg atgacttacc tgaagccggt actgccgaaa
     3361 ctgaccgagc gcgcagaagc atttctcaat acagaactga cctgggatgg tatccagcaa
     3421 ccgctgctgg gccacaaagt gaatccgttc aaggcgctgt ataaccgcat cgatatgaag
     3481 caggttgaag cactggtgga agcgtctaaa gaagaagtga aagcagctgc cgcgccagtt
     3541 actggcccac tggcagatga tccgattcag gaaaccatca cctttgacga cttcgccaaa
     3601 gttgacctgc gcgtggcgct gattgaaaac gcagagtttg ttgaaggttc tgacaaactg
     3661 ctacgcctga cgctggatct cggcggtgaa aaacgcaatg tcttctccgg cattcgttct
     3721 gcttacccag atccgcaggc actgattggt cgtcacacca ttatggtggc taacctggca
     3781 ccacgtaaaa tgcgcttcgg tatctctgaa ggcatggtga tggctgccgg tcctggcggg
     3841 aaagatattt tcctgctaag cccggatgcc ggtgctaaac cgggtcatca ggtgaaataa
     3901 tcccccttca aggcgctgca tcgacagcgc cttttcttta taaattccta aagttgtttt
     3961 cttacgattt tgtctctctc taacccgtat aaatactggt agcatctgca ttcaactgga
     4021 taaaattaca gggatgcaga atgagacact ttatctatca ggacgaaaaa tcacataaat
     4081 tctgggcggt tgagcagcag ggaaacgagc tgcatatcaa ctggggcaaa gtcggcacta
     4141 acgggcaaag ccagataaaa agttttgcgg atgttgcagc agcggaaaaa gcgaaactga
     4201 agcttatcgc ggagaaaacg aaaaaaggct atgtggaaaa tgcttcagca aacgtgcata
     4261 tcccccccat caccaaagcc actcctgaag ttgaaacttc ccctgagagt aaaaaccaac
     4321 gcccgtggct ggctgatgat gcagttattc cggtaactga cgatattaac cgatttgctt
     4381 ttcctcatcg ctctcgcccc agagagatca attatctgcg caaagacggt gagatatgga
     4441 agcgtattgc agataacacc agggcatatg atcccgacaa caattaccgc tcttatcctg
     4501 aaaactggca gcaggctttt gctgagttac aaaggcgaat tctggataat caacagacag
     4561 ggagtgcgca atctgatgca gcattgctct ggagtttttg gaactcttac tctgcagatg
     4621 aactggtcga tgacttagtc attcgctgtg gtctggaaag cgccgttgaa atcgtccttt
     4681 ttgcccttca actcaaatat aaaccagtaa aaggcgcagt aaccaccatc attccaccta
     4741 atcacgaagc agaatcgcta cctagttagc atcaacgtct atgtcatcat ctttcactcg
     4801 cttcagaaga tgagtggcaa cgctgtgtag ataaagtact caccgccatt ccgacgctat
     4861 cccccgcacg gcaacctttt gcggcgttac tgctccctga acgcccggat atcgcccatg
     4921 ctatagatct acgctatgca gaccaaaacg tcccggcgat gacctggtta agactggtga
     4981 taaccgatga tactgctctg agtacagtgg aaaaatacga cttcccaccg ttgtatcgtg
     5041 attttcgtaa ttttcgcgct tatctggcaa tgttgttggc aaataatggg gtgcgcggtg
     5101 taagccggat acttcttgag tttacggaag accattctga caatcccact tatctcttcg
     5161 aacgcatcag cgagactgaa aatcttgtca aatggctatg gaaaacgaat cacccggatg
     5221 cgattcaaat tctgatcctc ggtgtaattg gtaagaaaaa gcacctggaa tacttaagca
     5281 aggccagtca aaagcatccc gctgcggcta ttgcggctta tgctactttg ctggcaatac
     5341 atgaagataa agagtggcgt aaagcgcttg tcaaactgat taccgccacg ccagagttag
     5401 tatgcgacgt cattccctgg gttaatgcta aagcggcagg catcttatct gaatgtcgtc
     5461 cgcagtcagt cgctgaagaa tgtgaatatg ccactgtgga tatgttgccg gagctgcttg
     5521 tttcgccgcc ctggatgacc aaagagaaaa agaaaaatac gcctgtgttt gatcttccag
     5581 tgcttccagt tccttctgtt tctgatgtca ctccagagat taccaaaaaa ctgacccgta
     5641 cttaccttgt cacccacttc cagcaaatag cccaacagca ggcgacaaaa caaacattgt
     5701 ttaccgacct gccgccgatc aaaaaagcaa gctgggagaa acacttaata ccgcttacac
     5761 cggagcaaca aatactgtgg catcttggtt ttgaaaaatg gcgggagagc ggagaaaaaa
     5821 tatacgaaaa aattccggcg ccacaaagcg ccgttgatgc cctgctacgc ttcgattttc
     5881 cagcactcaa tgctgaattt gtgcattatc ataacaatgc atacaaaagc tggaatctca
     5941 ttgccctctg ttacctgcct ggtcaacagg ccatttcttt tcttaaccag atagtcaaag
     6001 aagataacta ctctggtgag ggaaatattc tggcaatttt tggtagcgcc gccatacctg
     6061 catttatggc ctgcctgcaa cgtgatccac gacgtctgtg cttcttcccc tttttcctcg
     6121 gtgtcagtga actggcctta ccgatggcgc aacaattaca gaaaaaaatg tcttatgaag
     6181 atgcgcgaaa ctggctaact gattatccac gccatgccgc cgccgggctg cttcctgtgg
     6241 ccctgggcaa aaaaggaaaa gatcgtgatt gtgcccgcca ggcgcttcgt ctgctggtga
     6301 atttaaacca gcgagaaacg attgaggaaa tcgcgcaagg atataatcaa cctgatgttt
     6361 tagctgcgct ggcaacattg ttcgatagcg acccactgga agaatatccc gctaaaatcg
     6421 ccccactgcc cggcttttat cagttcacct tgtggcgcag accacggctt aaaagtaaca
     6481 acctgcctct gtcagatgac gctatgcgcc acctcggcac tatgctgagc tttcctcgcg
     6541 acattaccgc ctacgctggg ctggatatca ttagagaaat cttcacccgc gaatcactgg
     6601 ctgaatttgg ctgggatctg tatcccgcct ggacagaagc tggcgcaccc gcaaaagaaa
     6661 actgggcatt tacttcgctt gggattttag gcaatgacga caccgcgcgt aaattaaccc
     6721 cgcttatccg cgcctggcct ggggaatccc agcataaacg ggcggtgtcc gggctggatg
     6781 tattagctga tattggcagc gatgttgcgc taatgctgct taatggcatc gcgcgaaaaa
     6841 ttaaattcaa agcattacag gaacatgccc gcgagaaaat caacatagtt gctgaaaacc
     6901 gtgggctgac tatggctgaa ctggaagacc gcctggctcc agatttaggg cttgatagca
     6961 gtggctcgct gatactggat ttcggccccc gcaaattcac cgttggtttt gacgaaacct
     7021 taaaacctgt agtgtgcgat gcaaacggca aagtcctgaa agatttacct aagccaaacc
     7081 agagcgatga aaaaactcag gcaactgacg cggttaatct cttcaaacag ttgaaaaaag
     7141 atgtacgcgc catagccagc cagcagattg atcgtctgga acaggctatg tgccagcgca
     7201 gacgctggac ggcagagcag ttccgcctgt ttctggtgga gcatccgctg gtacgtcact
     7261 taacccgacg gctgttatgg ggggtttata acgatgaaaa cgcccttatc acctgttttc
     7321 gcgtggcaga agacagcact tacagcgatg cgcaggatga gttattcacg ctgccagcag
     7381 gaaacatcgg tattccgcat gtgctggaaa tatcccctga atccgccgct gcattcaggc
     7441 aaatttacgc tgattacgaa ctgcttcccc ctttccaaca gctcgagcga ggtagctatc
     7501 accttgctga taatgaacgt aatactcacg aactgacacg ctggcaagga cggctttgcc
     7561 aggccggacg cattgtcggg ctggaacgca gaggctggca acgtctggaa gaaagcggca
     7621 gcgtttatgc gatgcgcaaa acaacccctc atggcgatct cgaacttgaa acggaacctt
     7681 tttcattaat ttatggtgaa acggggtatg gcgaccagca cccggttgaa agcgtaaaaa
     7741 tcacctcgcc agatgatcgc tacggtaaac aatcctcact cactttctcc atgctcgacg
     7801 acatcaccgc cagcgagctg attaacgaca ttgaatcact gtttgattaa aaggatttaa
     7861 caatgaaaac ctatatttat caggacgaaa aatcacataa attctgggcg gtggagcagc
     7921 agggaaacga gctgcatatt aactggggta aagtcggtac caacgggcaa agtcaggtaa
     7981 aaagctttgc agatactgcg gcggcggaaa aagcagcact gaagctgatt gcggagaagg
     8041 tgaagaaggg gtatgtggag caagtagacg ctaacgctca ctcacatgat gcctcagcaa
     8101 agaaagtcct ggtcacagag gaaaaagcct caataaatcg ccaggcagca actaatggtt
     8161 taccctggct ggcagacaac gatcccatta ttcttccccc gaatattgcc cgccatgccc
     8221 tcagccaccg cctttggccg ggagagccag taaaaaaacc aaccaaaccc gagaaacaaa
     8281 atttaatttc ccgattggca aatcgtactt atcattatta taaaaatctg actttcgatt
     8341 acagctcatg ctctgatgaa tgggctgatg cgattagcca ggcaatcagc ttaattaagg
     8401 cccaaaagac tgagataccc gcgcgtacta tggcggtact tacttttctg gaaatatgtt
     8461 tttgtcaggg ctggaatgat gaaacctcat ccgagttgct ggatgaaatt gtaagcacct
     8521 tcggtatcga atacgcaact gaaactgtca tcttctggtg gcaaattgat ttcgattttg
     8581 actataaaga tgaacatttg actttcttta ttaattatgc agatagcagc aaggacagtt
     8641 atagaaggaa taacgacaaa tttagtttac gtctgcgtaa acatttatca ctagcagaag
     8701 aagaagtatg gcaaaattgc attgcaaaat tactcgttgc gttaccagat atatcactct
     8761 atcgccaacc gttaattgcc attttaatgc cagaaatacc cgaagtggcc catgagatcg
     8821 tacatcgttt acaccaggtc gcagatgttc cacaacttga aatgttgaaa ctggtagcga
     8881 cagatccatt cacgctcgaa atactggaga actatcaata cattgatgta tttaacacct
     8941 atggtgccag ttggtcagct acggtattac gggagcaagg tatagcggca ctggctcgcc
     9001 tgactcccta tgctgaagaa gataattgcg gtgacgtact gaaatgtatt aatcatccgt
     9061 tggccattac gaataaaaag tgccgaaaat tttccttctg caacgatagt ggcattagcc
     9121 gaagcattgg taaaaaaaga tgataaacgc aaacatacat tgctggtttc attacttaat
     9181 gcaaatcctt tatttgtcga aaaattagtc atcggattat cgtcgcaagc ggcaaattta
     9241 ttgcaacaat gtcagcaatt gctgaatagt cgcaatgaag attttgcaaa ctcagaaatt
     9301 ctaccaatgg tactagtctc gccgccctgg gaaactcaaa ataaaaataa cgacaacagt
     9361 cctgtgggta aatacccggc gaaaatcccc cctttaccgt tgttttatca acctccatta
     9421 tggactcgcc cgaaactcaa aaatagcagc aaggtactgc cagatagcgc actccagcac
     9481 ctcggtgaaa tgctccgctt ccctcaggaa gaggccctgt atccgggatt attgcaggtg
     9541 aaagatgcct gtaccgccga ctcactggct gaatttgcct gggatctgtt taccgcctgg
     9601 ctgactgcgg gcgcaccgtc gaaagagagt tgggcgttca ctgcgttagg cgttcttggt
     9661 aacgatgaca ccgcccgcaa actgacgcca ttaatacgcg cctggcctgg tgaatcccag
     9721 cataaacgcg ccaccgttgg gttggatatt ctcgccgcta tcggtagtga tatcgccctg
     9781 atgcagctta acggtattgc ccagaaactg aaattcaaag cattacagga gcgggcaaaa
     9841 gaaaaaattg ccggcattgc cgaaagccgc gaactcacgg tggcagagct tgaagatcgg
     9901 ttagcaccgg atctcggtct ggatgataac ggttcgttgc tgctggattt cggctcacgc
     9961 cagttcaccg tcagctttga tgaaacctta aaaccgtttg tgcgtgatgt ttctggcagc
    10021 cgtctgaaag acctgcccaa accaaacaaa agcgatgatg aatcacaggc gaacgatgct
    10081 gttaaccgct acaaactgct gaaaaaagat gcgcgtaccg tcgccgccca gcaggtagca
    10141 aggttggaat ccgccatgtg cctgcgtcgc cgctggtcgc cagaaaactt tcagctcttc
    10201 ctggttgaac atccactggt acgccactta acccgccgtc tgatttgggg cgtttatagc
    10261 accgaaaacc agctactgac gtgcttccgc gtggcggaag ataacagcta cagcaccgct
    10321 gacgatgatc ttttcaccct gccggaaggc gatatctctg ttggcattcc tcacgttctg
    10381 gaaatatcac cgacggatgc taccgccttt ggtcagcttt ttgccgacta tgaactgcta
    10441 ccaccgttcc gtcagcttga ccgtaacagt tacgccctga cagaagccga gcgcaacgcc
    10501 agcgaactga cccgctgggc aggcagaaaa tgcccgagcg gtcgggtcat ggggctggcg
    10561 aataaaggct ggataaaggg caccccgcag gatggaggct ggatcggctg gatgatcaaa
    10621 cctctgggcc gctggtcgtt aatcatggaa atcgatgaag gttttgcggt aggcatgtcg
    10681 ccagccgaac tgagtgccga gcaaatctta agcaagctgt ggctatggga aggcaacgca
    10741 gaaagctatg gctgggggag taattcaaca caggaagcgc agttctccgt actcgatgcc
    10801 atcaccgcca gcgagctaat taacgatatt gaagccctgt ttgaataagg aaagagcatg
    10861 gacaaggaat taccgtggct ggcggataac gcccaactgg aactgaaata taaaaaaggc
    10921 aaaacgccgc tcagtcatcg tcgctggccg ggcgaaccag tgtccgttat cactggaagt
    10981 ctcatccaga cattgggaga tgaattacta caacaagcag gccagaagga aaatatcacc
    11041 tggaattatg ataaatgctc actggagtgg cagtccgcca tccagcaggc aatcaatttg
    11101 actggcgagc ataaaccgtc aatcccagcc cttaccatgg cggctctgat ctgtatcgcg
    11161 caaaatgaca gccagcagtt acttgatgaa atcgtgcaac aagaggggct ggaatatgcg
    11221 acggatgtag tgatatcgcg tcagtgcatt gcgcggcgtt atgagagtga ctcactggtc
    11281 gtcactttgc agtatcagga cgaggactat ggctacggtt atggctccgc gacctataac
    11341 gatttcgatc tccgtttgcg taagcatctg tcgctggcag aggaaagttg ctggcagcgt
    11401 tgtgccgata aactcattgc cgcactgcca ggaataccca aaattcgccg tccttttatt
    11461 gcgctgatcc tcccggaaaa acctgaaata gccaatgaac tcgccagtct tgaaagctcc
    11521 cggtcaagtc tccattcaaa ggagtggtta aaggtcgttg ctactgataa tacggcggta
    11581 aaaaaactcg aacgatactg gggcctggac gtatttagcg atcgcgaagc cagctacatg
    11641 tcacaagaaa accgcttcgg ctacgcggcc tgcgcctccc ttttgcgcga acaaggactg
    11701 gcagccgttc cgcgcctggc aatgtatgct cataaagaag attgcggcag tctgctggta
    11761 caaattaacc atccgcaagt catccgcacc ttgctgttag ttgctgataa aaacaaaccc
    11821 agcctgcaac gtgtggctaa atacagtaaa aacttccccc atgcgacgct cgccgcactg
    11881 gcagaactgc tggcgttaaa agaaccacca gcccgccctg gttatccaat catcgaagac
    11941 aaaaagctgc ctgcacagca aaaagctcga gaagaatact ggcgtacgct gttacaaacg
    12001 ctgatggcat cgcagccaca actggcagca gaagtgatgt cgtggttaag tactcaagcc
    12061 agggcagtgc tgaagagtta tttatcggca ctgccaaaac cggttattga tggcaccgat
    12121 aacagcaatc tgcctgaaat cctcgtttca ccaccgtggc gtagtaagaa aaaaatgaca
    12181 gctccacgtc ttgatttggc acggctcgaa ttaactccgc aagtttactg gcaaccaggc
    12241 gaacgagaga ggcttgccgc cactgagtct gcccgttatt tcagcacgga atctcttgcg
    12301 caacgcatgg aacaaaaaag tgggcgagtt gtattacagg aactgggttt tggggatgat
    12361 gtatggctgt ttttgaatta tatactccct ggaaaactgg atgctgcacg caattcactc
    12421 attgttcagt ggcattacta ccaggggcgg gttgaagaga tcctgaatgg ctggaactcc
    12481 ccggaagcac aattagcaga acaggcgctc cgcagcggtc acatagaagc gttaattaac
    12541 atatgggaaa atgacaacta ctcacgttat cgtccggaaa agagtgtctg gaacctgtat
    12601 ttattggcac agttgccgcg tgagatggct ttgactttct ggctgcgtat caatgagaaa
    12661 aagcatctgt tcgcgggaga ggactatttt ctcagtatcc tcggattgga tgcgctacca
    12721 ggtctgctgt tggctttttc acatcgtcca aaagaaacat ttccgttaat tttaaatttc
    12781 ggcgcaacag aactggccct gcccgttgcc cgcgtctggc gacgtttcgc ggcgcagcgt
    12841 gatctggctc gccagtggat tttacaatgg ccggaacata cggctactgc acttatccct
    12901 cttgtcttca ccaaatctag cgataaaagc gaagctgcat tacttgccct gcgtttactt
    12961 tacgaacatg ggcatggcga attgctacaa actgtagcca atcgttggca gcgtacagat
    13021 gtatggcctg ccctggagca tttactgaaa cagggtccca tggaaattta cccggcacgc
    13081 attccaaaag cccctgattt ctggcatccg caaatgtggt ccaggccgcg ccttatcact
    13141 aataatcaac ctgttaccga tgacgctctg gaaattatcg gcgaaatgct gcgctttacc
    13201 caggggggac gtttttatag cgggctggaa caactgaaaa cgttctgcca gccacaaacg
    13261 ctggcagctt ttgcctggga tctcttcact gcgtggcaac aagctggtgc ccccgcaaaa
    13321 gacaactggg catttctggc gttaagtctc tttggtgacg aaagcacggc acgggatcta
    13381 acaacacaga tcctcgcctg gccacaaggc aaatctgccc gtgctgtcag cggcctgaac
    13441 atccttaccc tgatgaataa tgatatggcg ctgatacagc tgcatcatat atcgcaacgg
    13501 gctaaatccc gccccttacg tgataacgcg gcggaatttc ttcaggtagt cgcagaaaat
    13561 cgcgggctaa gccaggaaga gctagcggac agattagtcc caaccctggg ccttgatgat
    13621 ccgcaggcgt tgagttttga ttttggtccc cggcagttta ccgttcgctt cgatgaaaac
    13681 ctcaacccgg ttatctttga tcagcaaaac gttcgccaga aaagcgttcc ccggttgcgc
    13741 gccgatgacg atcaactgaa agcgcccgag gcactggccc gactaaaagg gctaaaaaaa
    13801 gatgctactc aggtgagcaa aaacctgctc ccgcgtcttg aagctgccct acgtaccacc
    13861 cgacgctggt cgctggcaga ttttcattct ctgtttgtta atcatccctt tacccgtctg
    13921 gttacccagc gattaatatg gggggtttat ccggcaaatg aaccgcgtcg tttactcaac
    13981 gcctttcgtg tggccgcaga gggggggttc tgcaatgcgc aagatgagcc aattgacctg
    14041 cctgcggacg ctctgattgg cattgcccac ccgttagaaa tgacagcaga aatgcgcagt
    14101 gaatttgcac agctttttgc cgattacgaa attatgccgc cttttcgcca gttggcgcgc
    14161 cgcacggtgc tgctcacacc tgatgagtca accagtaaca gcctgactcg ctgggaaggt
    14221 aaatccgcta ccgttgggca acttatggga atgcgataca aaggctggga gtcaggttat
    14281 gaggacgcat ttgtctatga cctgggtgag taccggctgg tccttaagtt ttcacccggt
    14341 tttaaccact acaatgttga tagcaaagcg ttaatgagct tccgttctct tcgagtgtac
    14401 cgtgacaata aatccgtcac ttttgccgaa cttgatgtgt ttaatttgag tgaggcgtta
    14461 agcgcacctg acgtcatttt ccattaaaaa cacagcgggc agtgatgcaa ctgcccgtta
    14521 tcaacacgac ttaccagcgg ggaaagcgat gatcgtgcaa aaagagctgg ttgctattta
    14581 cgattatgag gtccctgtac ctgaagatcc gttttccttc agacttgaga tccataaatg
    14641 ctctgaatta tttacaggtt ccgtctatcg actggagcga ttccggctac gttcaacatt
    14701 tcatcaacgt gatcgagaag atgctgaccc gctaataaat gatgcgttga tttatataag
    14761 agatgagtgt attgatgagc ggaaattacg aggtgaatca cctgaaactg taatagcaat
    14821 ttttaatcgt gaactacaga atatattcaa ccaagaaata gaataatata ctctagataa
    14881 ttaaagtcgc agcacatgca acttgaagta tgacgagtat agctttctta aagggggaaa
    14941 cggtattccc ccttagatgc gtttcaattt gcccgttact tcaacaaatc aatcaaccgt
    15001 aacagagcca ctaataactc cagtacggca atgagcacgg ttaacttttg tgtcagcgtg
    15061 tccatccgct ctccttatta aggagcgcaa caccagcacc ttacccttac actgcctgtc
    15121 gatactggtt tacgtatgtt ggtacgaaac gttgctctcc ggatcagggc accgcctgcg
    15181 gcaccacccg tccggcagcc aggacttaaa attggtccga tccggcacaa cctcccaacg
    15241 ggcaggatta taaatctcac tcactgaatt gcaatggagt gtggatatgg ttataattta
    15301 ttcgttggta cgaaatgttg cacaatcagc aagttagcgt cactgtagta aataaaagaa
    15361 cctgcctcac cagcaggttt ttttattttc tgcgatctac tttccagata tttttttgcc
    15421 aatagcccac cataaatatt cacatcccgg tcaaatggtg cgttgaatat aaaatcgatg
    15481 aatttataaa taaaaatgag ttatatgatg aattaataaa aacagtaatc attttattaa
    15541 agggggaagc ggttttcccc cttagatgcg tttcaatttg cccgttactt caacaaatca
    15601 atcaaccgta acagagctac taatagctcc agtacagcaa tgagcacggt taacttttgt
    15661 gtcagcgagt ccatccgctc tccttattaa ggagcgcaac accagcacct tacccttaca
    15721 ctgcctgtcg atactggttt acgtatgttg gaccacgatg ttgcactccg gatcagggca
    15781 ccacctgcag caccccccgt ccggtagcca ggacttgaaa taggttcgat ccggcgcaac
    15841 gtcccaacag tcaggattat aaatctcatt atcttaattg caacggggtc cagccgtggt
    15901 tataattcgt tcgttggaac acgatgttgc acaggctgtg gtgttaggcc tgaaaatagt
    15961 aaataaaaga acctgcctca ccagcaggtt tttttattta ctgtgatctg cttcccagat
    16021 atttttcgct caaacaacta atgcgccaaa catttattgc gcgtaaaata tcgtttattt
    16081 cattaataca tttcagggat gaatatatgt caccgcagaa taatcatctg cagcgtccgc
    16141 ctgctgctgt gttatacgcc gatgaactgg caaaattaaa acaaaatgat aacgcacctt
    16201 gcccgcccgg ttggcagtta agtttgcctg cggcccgtgc ttttatcctt ggcgatagcg
    16261 cgcaaaatat tagccgtaaa gtggtgatta gtccctccgc tgtcgaacgt atgttagtca
    16321 ctcttgccac cggacgcggt ttgatgttgg tgggggaacc tggcaccgca aaatctctcc
    16381 tgtctgaatt actggcaacc tctattagcg gcgatgccgg gttaaccatc cagggcggcg
    16441 catctactac cgaagatcaa atcaagtacg gctggaacta cgccctgctt atcaatcatg
    16501 gtccatcaac ggaagcgctg gtccctgccc cgctttacca gggaatgcgc gacggcaaaa
    16561 tcgtccgttt tgaagagata acgcgtacac cgctggaagt gcaagactgt ctgttaggta
    16621 tgctttctga cagggtgatg acggtgccgg aactcactgg tgaagccagt cagctctatg
    16681 cgcgagaagg ctttaatatt attgccactg ccaatacccg cgatcgcggc gtcaatgaaa
    16741 tgagtgctgc gctaaagcgt cgcttcgatt ttgaaactgt ctttccgatt atggatttcg
    16801 cccaggagct ggagcttgtc gccagtgctt cggcgcgttt gctggcccat agcggtattc
    16861 cgcataaagt gcctgacgcg gtactggagt tactggtccg caccttccgc gatctgcgtg
    16921 ccaacggcga aaagaaaacg tcaatggata cactaacagc gattatgtcc accgctgaag
    16981 ccgtgaacgt ggcccacgct gtgggcgtcc gcgcctggtt tttagcgaat cgcgcggggg
    17041 agcctgccga cctggtggac tgtattgccg gaaccatcgt caaagataac gaagaagatc
    17101 gcgctcgtct gcgccgttac ttcgaacagc gtgtcgccac acataaagaa gctcactggc
    17161 aggcttatta tcaagcccgc caccgcctgc cgtgaggaaa gatgcatgag cgagccgtta
    17221 attgtcggca tccggcatca tagtccggcc tgcgcccgac tggtgaaatc gttaatcgaa
    17281 agccagcgac cacgatacgt gttgattgaa ggcccggctg attttaatga ccgggtagat
    17341 gaactgtttt tagcccacca gcttccggtg gctatttaca gttattgcca gtatcaggac
    17401 ggtgctgctc ctgggcgcgg cgcctggacg ccatttgccg aattttcgcc ggagtggcag
    17461 gcgctacaag ccgcacgtca tattcaggca caaacttact ttatcgattt gccttgctgg
    17521 gcgcagagtg aagaagagga cgattcgcct gatacgcaag aggaaagcca ggccttgctg
    17581 ctgcgtgcca cccgcatgga taacagcgat accctgtggg atcacttgtt cgaagatgaa
    17641 agccagcaaa ctgcattacc ctctgcactg gcgcactatt ttgcccaact gcggggcgat
    17701 ttccccggcg atgcactcaa tcgtcagcgc gaagccttta tggctcgctg gattgcatgg
    17761 gcagtgcagc aaaataatgg cgacgtgtta gtcgtctgcg gtggctggca cgctccggca
    17821 ctggcaaaaa tgtggcgcga atgcccgcag gacattaaca agccagaatt gccctcgctg
    17881 gcagatgccg ttacaggttg ttatctcaca ccctacagtg aaaagcgcct tgatgtgctg
    17941 gcaggatacc tttcaggaat gcctgccccg gtctggcaaa gctggtgctg gcagtggggc
    18001 ttacagcagg cgggagaaca actgctaaaa acggttctta cccgtttgcg ccaacaccac
    18061 ttgcctgctt cgaccgcgga tatggctgcc gctcatctgc atgcgatggc gctggcacag
    18121 ttgcgcggat ataaactacc gttacgcact gactggctgg atgccatagc aggctcgctg
    18181 attaaagaag ccctgaacgc gccgttgccg tggagctatc gcggcgttat tcatcccgat
    18241 accgatccga ttctgctaac gttgatagac acattagcgg gtgacggatt cggtaaactt
    18301 gccccttcta cgccacaacc gcctctgcca aaagatgtca cctgcgaact ggaacgtacc
    18361 gcaatctctc tttcggcgga gcttacctta aatcgcttta accccaatgg actagcgcaa
    18421 agtcaggtgt tacatcggct ggcaatactg gagatcccag ggattgtacg ccagcaggga
    18481 agcacactga cacttgcagg caacggtgaa gaacgctgga aattaacccg cccgcttagc
    18541 cagcatgcgg cattgattga ggccgcctgc tttggtgcca cactccagga agccgcacgc
    18601 cataaattag aagccgatat gctggacgcg ggtggaatcg gcagtatcac cacatgtctt
    18661 agccaggcgg cgttagcggg tctggcgtcc ttcagtcaac aattactgga gcaactcaca
    18721 ttattaatcg cctaggaaaa tcaatttgcc gaaatgggcc cggcgctgga agtgctatat
    18781 gccttatggc ggctggatga aattagcggt atgcaaggtg cgcagatatc acaaacgacg
    18841 ttatgcgcgg ctatcgatcg cacgctgtgg ctgtgtgaat ctaacggcag accggatgaa
    18901 aaggagtttc acgctcacct gcatagctgg caagcgcttt gccatattct gcgcgatcta
    18961 catagcggcg ttaatttacc cggcgtttct ctttctgcgg cggtagcctt actggagcga
    19021 cgcagtcagg caatccatgc cccggcgctg gatcgcggcg cggctcttgg cgcactgatg
    19081 cgtctggaac atcccaacgc tagtgccgaa gcggcgctga cgatgctggc gcagttatcc
    19141 ccggcacaat ccggcgaagc gctgcacggt ttgctggcgc tggcccgcca tcaactggcc
    19201 tgtcagccgg catttatcgc cggtttcagc agtcatttaa atcaaccaag tgatgccgat
    19261 tttatcaatg ccctgcccga tttacgcgcg gcgatggcct ggctaccgcc acgagaacgc
    19321 gggacactgg cgcatcaggt gcttgaacat tatcaactgg cgcaacttcc cgtttcggcg
    19381 ctgcaaatgc cgttgcattg cccaccacaa gccattgcac atcatcaaca actcgaacag
    19441 caggcactgg catcgctgca aaactgggga gttttccatg tctgaactga acgatcttct
    19501 gaccacccgt gagctacaac gctggcgatt aattcttggc gaagcggcag aaacgacgct
    19561 ttgtgggctg gatgacaacg cccggcagat agaccacgcg ctggagtggc tgtatgggcg
    19621 cgatcctgaa cggctccagc gtggtgaacg ctccggtgga ttaggtggct caaatctcac
    19681 cacccctgag tggatcaaca gtattcacac gctgtttccg caacaggtga ttgagcggct
    19741 ggaaagcgat gccgtactgc gctacggcat tgaagatgtg gtgacgaatc tcgacgtgct
    19801 ggaacgtatg cagccttctg aaagcctgct acgcgccgtt ttgcacacca aacatctgat
    19861 gaaccccgaa gtactggctg ccgcccgccg gatagtgcgc caggttgttg aagaaattat
    19921 ggctcgactg gcaaaggaag ttcgtcaggc tttttctggt gtccgcgatc gccgtcgccg
    19981 ctcatctatt ccactggcgc gagactttga tttcaaaagt actcttcgcg ccaatctgca
    20041 acactggcac ccgcaacacg gcaagttgta tatcgaatcc ccccgcttta acagccgaat
    20101 taagcgccac agtgaacaat ggcaactggt cttactggtt gatctaagcg gatcgatggt
    20161 cgattcggtg atccactctg cggtaatggc ggcctgtttg tggcagttac ccggcattcg
    20221 tacccatctg gtggcgtttg acaccagtgt cgttgatctc acggcagacg ttgccgatcc
    20281 ggtagagtta ttaatgaaag tacagttggg cggcgggacc aatatcgcca gtgccgtgga
    20341 gtatgggcgg caacttattg aacaaccagc aaaaagcgtc attatcctcg tgagtgattt
    20401 ttatgaaggg ggttcatcat cattgctgac gcatcaggtg aaaaagtgtg tccagagcgg
    20461 catcaaagtg ctggggctgg cagcgctcga cagcaccgca acgccttgct atgaccgcga
    20521 tatggcccag gcgctggtta atgttggcgc acaaatagcc gcaatgacac cgggtgaact
    20581 ggctacctgg cttgcggaga atttgcagtc atgaattcac tacgtccgga attattagaa
    20641 ctcacgccgc aggccctgac ggcgttaagc aatgccggtt ttgttaagcg cagccttaag
    20701 gaactggaaa atggcaacgt cccggaaatc agccatgaga acggcgcttt aatcgccacc
    20761 ttcagtgacg gtgtccgtac ccagctggcg aacggccagg cactgaaaga ggctcagtgc
    20821 acctgcgggg ctagcggcat gtgccgtcat cgcgtgatgc tggtgttaag ttatcagcga
    20881 ctttgtgcca ccacccggcc cacggaaaaa gaagaagagt ggcagcctgc aatctggctg
    20941 gaagaactgg ctaccctgcc cgatgccacc cgcaaacgtg cacaggtgct ggtcgctaaa
    21001 ggcatcacca ttgagttgtt ctgtgcgcct ggcgaaattc cctcagcccg cttaccgatg
    21061 agtgatgtgc gtttttattc ccgcagcagc attcgcttcg cccgctgtga ttgtattgaa
    21121 ggcacacttt gcgaacatgt cgtactggct gtgcaggcct tcgtcgaggc caaaactcag
    21181 caagcggaat ttactcattt aatctggcag atgcgcagcg aacacgtcac atcatctgac
    21241 gacccgtttg ccagcgaaga aggcaacgcg tgtcgtcaat atgttcagca attaagccag
    21301 gcattatggc ttggcggcat cagccagccg cttattcact acgaggccgc tttcagccgc
    21361 gcgcagcaag cggcggaacg ctgcaactgg cgctgggtga gtgaatcgct acggcaactg
    21421 cgcgccagcg ttgatgcctt ccacgcccgc gccagccact atcatgcagg agaatgctta
    21481 cgtcagcttg cggcattaaa cagtcgatta aattgcgcac aagagatggc ccggcgcgac
    21541 agtgttggtg aagttcctcc tgtgccgtgg cgcacggtcg ttggctctgg cattgccgga
    21601 gaagcaaagc ttgatcatct gcggctggtg tctttaggta tgcgttgctg gcaggatatt
    21661 gagcattatg gtttacgcat ctggtttacc gatcccgaca ccggcagtat tttgcacctt
    21721 tcgcgcagtt ggccgcgaag tgaacaggaa aactcaccgg cagctacgcg tcggctgttt
    21781 agttttcagg ctggcgcact ggcgggtggg caaattgttt cacaagcagc aaaacgcagt
    21841 gccgatggcg agctgctgtt agctacccgc aaccgcttaa gcagcgttgt gccgctgtcg
    21901 cctgatgcct ggcgaatgtt gagcgcgccg ttacgccagc cgggcattgt ggctttgcgg
    21961 gaatatttac gccagcgtcc cccctcctgc atacggcctc ttaatcaggt cgataactta
    22021 tttattctgc cggtcgctga gtgtatttcg ctcggttggg atagcagccg ccagacgctg
    22081 gatgcgcagg taatcagcgg cgaaggtgaa gataatctgc tgacgttatc actgccagcc
    22141 tcagccagcg cgccttatgc cgttgaacgc atggcggcgc ttttgcaaca aacagacgac
    22201 cccgtgtgtc tggtttctgg ctttgtcagt tttgttgatg ggcaattgac actggaacca
    22261 caggtaatga tgacaaagac tcgtgcctgg gcgctggacg cagaaactgc gcctgtggtg
    22321 gcatcactac cttctgccag cgttctgcct gtgccgtcta ctgctcatca gttgctgatg
    22381 cgctgccagg cattacttat tcaactgctc cataacggct ggcgctatca ggaacagagt
    22441 gctattggtc aggcagagtt gctggcgaat gacctcaccg cggtgggttt ttatcggctg
    22501 gcacatgtat tgggacaatt tcgtaataca gaaagcgagg cacgggtaga agcaatgaat
    22561 aacggtgttt tgctgtgcga acaattattc cccctgcttc agcaacaagg atgaaatagt
    22621 gctttttact aagagttcta ctccagttcc ggactgctca cgccacggta ttaggcatat
    22681 cctatatagc ccctggtgag agtcaccagt tccttgatta aataaaatgg agttttacat
    22741 gaaggctttc aataagctgt tttcccttgt tgttgcatct gttctggttt tttctcttgc
    22801 tggctgcggt gacaaagaag aatcgaagaa attcagcgcc aatctgaacg gcactgaaat
    22861 tgccattacc tatgtctaca aaggtgacaa ggtgcttaag caatcttctg aaaccaaaat
    22921 tcaatttgcc tccattggtg ctaccaccaa agaagatgct gccaagacac ttgagccgtt
    22981 aagcgccaaa tacaaaaaca tcgcgggtgt tgaagaaaaa ttaacctata ccgataccta
    23041 cgcgcaggaa aacgtgacta tcgatatgga aaaagtggat tttaaagccc tgcagggtat
    23101 ttcaggaatc aacgtttctg ctgaagatgc caaaaaaggt atcactatgg cgcaaatgga
    23161 actggtgatg aaagccgctg gttttaaaga agtgaaataa tctgtcggcg gccatgtttg
    23221 cgcatggccg ccatacccgt cttagctttt cttcacatgc tggcgcgctg ccagtccacg
    23281 cagaaaataa cgtaaaaatt gatcgccgca ttcgcggaag tttttatgat ccggtgcacg
    23341 catcatcgcc gtaatttccg gcatcgaaac gcggaactgc tgttcggtga ggatcgccag
    23401 aatgtcatcg gttttcagcg aaaacgcgat gcgtaatttt ttcagcacga tgttgttatt
    23461 aatgcgacgt tccggctcca gtgccggagc agactcatcc ttgccgcgtt tttcataaat
    23521 caggccattg aggaatgacg acaaaacaat gtccggacaa cgctgaaaac cctcttcgtc
    23581 ttctttacgt agccagacgg cgatctgttc cgcggtggct tcgacattac ccagcgccag
    23641 tatacgcacc aggtcattat tattggcttt caaaatgtag cgcacgctgc gcagaatatc
    23701 gttacttagc atgaggcctt caggtgttga tgaggcaaaa agccatttta gcagtctttt
    23761 acaggccaat cgcctctttt aagcttttca gataacggcg gctgaccggc acggttaagc
    23821 cattacgcaa aatcaactcg gcctgaccgt tatcttccag acgaatctcc tgtaaatgcg
    23881 cgaggttaac cagatactga cgatggcagc gcagtagtgg tgtacgactt tccagggtac
    23941 gtaatgtcaa ttcggtaaag ccctctttcc cttcgtggct ggtaacgtag acaccgctca
    24001 tccgactgct gacaaatgcc acatctttca tttgcagcaa ataaatccga ctatgccccg
    24061 tacaagggat aaatttcagc gcctgttgat tttccggtaa cagcgaaaca tcctgcttgc
    24121 tgcgctcctg acgcaatcgc gccagcgttt tctccagtcg cgcttcatca attggcttca
    24181 gcagatagtc aaaggcatgt tcttcaaagg ctttaatagc gtattcgtca aacgcggtga
    24241 gaaaaacaat atacggacga tgttccgggt cgagcatccc caccatttcc agaccactga
    24301 tgcgcggcat ctggatatcg agaaacagca catccgggcg cagtttatgc accgcgccga
    24361 tcccttccac ggcgtttgaa cactcaccaa cgatttcaat atcgctctgc tcctgcaaaa
    24421 atacgcgcag gttctcccgt gctaacggtt catcatcgac aattaagact ttaatcatgc
    24481 ctcgtccctc catggtagtc gtaacgttat tcgggtgtaa ctatcaggct cacaggcgac
    24541 gcttatccca tagtcatcgc caaaccgttc acgtaaacgc ttatccacca gattcatccc
    24601 cagcccactg gcattggtta ccggttgata caaaccggca ttgtcttcga tctccagcat
    24661 caaatgttgc ccctcacgtc ggacgctgat tgccactcgc cctgtatcca gcagttgtga
    24721 tgtcccatgt ttaatggcgt tttccactat cggttgcagg gtaaacgcgg gcaattgctg
    24781 ctgggataat tcttgcggaa tagcaatgtt gacctgcaac cgcgactgga agcgcgcctt
    24841 ttcaatttgc agataagcat tcacatgttc aatttcgtcg gcgagagtaa caaactccga
    24901 aggccgcttt aagtttttgc ggaaaaaagt ggaaagatac tgcaccagct ggctggcctg
    24961 ttcgctgtcg cgacggatca ccgctttaat ggtgttaagc gcattaaaca aaaaatgggg
    25021 gttcacctgg gcgtgaagca gtttgatctc tgactgggtg agcatcgctt tttgccgctc
    25081 atattgcccg gcgaggatct gcgccgaaag cagttgcgca atcccctcgc ccagcgtgcg
    25141 gttgattgaa ctaaataaac ggtttttggc ttcatacaat ttgatggtgc ccatcacccg
    25201 ctgattttca ccacgcaacg gaattaccag cgtcgacccc agtttgcatt gcggatgcaa
    25261 agagcaacga taaggtactt cgttgccatc agcgtagacc acttcaccgg tttcaatcgc
    25321 ttttaaagta taagttgaag aaatcggttt gccgggtaaa tggtggtcgt caccaattcc
    25381 ggtaaaggcc agcaatttct ctcgatcggt aatcgcgact gcaccaatat ccagctccag
    25441 atacagcacc tgagccactt tcatgctgtt cacttcgtta aacccctggc gcaaaatgcc
    25501 ttccgtcgag gctgccactt tcagcgcagt ggcagaaaaa gccgaagtgt atttttcaaa
    25561 catcgcgcgt ttatcgagca atatacgcat aaacagcgcc gcgccgacgg tattggtgac
    25621 catcattggc gcagcaatat tactcaccag acgcaccgca tcttcataag gtcgggcgat
    25681 cgccaggatg atcagcattt gcaccatttc agcgacgaac gtgacggcac cagcggtaat
    25741 ggggttaaag actttatcag tgcgcccgcg acggatcagg atgctgtgta ccaggccgcc
    25801 gagcaatcct tcaacgatgg tcgagatcat gcaacttagc gcggtcatgc cccccatcga
    25861 atatcgatgt aacccgccgg tcaggccaac cagcccaccg acgaccggac cgccgagtaa
    25921 gccgcccatt accgcgccta tcgcacgggt attggcaata gaatcgtcaa tgtgcaaccc
    25981 aaaccaggtg cccatgatgc agaagatgga aaagacgatg tagcagagaa atttatgcgg
    26041 cagacgaacc gtgacctgca ttaacggtat gaataatggc gttttactca ttaaccacgc
    26101 aatgactaaa aaaacgcaca tctgctgaag cagcagcaac accagattaa aatcgtacat
    26161 acccgcaaac cacacatccc tttaaaacgc gtaacataca ttgcctgcgt ttaactttct
    26221 ttgaactctt gcagaaaaat gagaattcgt gagtacgatc actcaaaatc gcctggcaaa
    26281 aataaaatca ccctataaat gcacaaaaaa cgggcaaaac tacctggttc gcaaaactgc
    26341 gtctaaagtt aaaccgggac ctcgcgagca agggtgagac gatggcgctt tacacaattg
    26401 gtgaagtggc gttgctttgt gatattaacc ctgtcacgtt acgcgcgtga cgcaacattt
    26461 ttaaaaatcg acgaagatcg tccaggagcg ccgtttttca agggttggat agatgattcg
    26521 tttaccatgt cgatgaactt cgacgaattt cgccgataaa aagtgtccca tacatgcccc
    26581 attgatgccc cataaacaat atcaggaatt gacactggtt atccatacag ataaaaataa
    26641 cacctgtaca taactacagt tacaggaggg tgttatgcgt gttgagatct gtatcgctaa
    26701 agaaaaaatc actaaaatgc caaacggtgc tgtggatgcg ttaaaggaag aattaacccg
    26761 acgcatcagt aaacgttatg acgatgtaga ggtgatcgta aaagccacca gcaacgatgg
    26821 cctttctgtt acgcgcaccg ccgataaaga ttcagctaaa acttttgttc aggagactct
    26881 gaaagatacc tggaagtctg ctgacgagtg gtttgttcac taattagcac gtaaaatcgg
    26941 taacggctgg aaatcatcca agactagcac tatcgaaagt tcaccaacca accgcagtac
    27001 gttcttgcat acgaggtgcc gcgttttaca tgccgtaccg agaattaatt tcattttacg
    27061 aaaagttaac ccgccattgc taccttaatt ttttaaaacg aagttacctc tgtcagggtc
    27121 aaaagcacaa ttatctcttc ctacaatcat tgaaactgcg attgattccc tgctcaaagg
    27181 atgagccgcc cttgttttaa caagatgtat taatgccatt ttatcataca acatacaaac
    27241 atttgaattc attgaattcc tcataaatac cccatcttct ggttcacata atgtaacagg
    27301 gcatgttcgt aagctctctg gcacttgcaa ttgtgcatgc tccacattaa atgcacatac
    27361 ttcaatttta tctaacaact cctc
//
LOCUS       DAZFAB010000055        27370 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZFAB010000055 DAZFAB010000000
VERSION     DAZFAB010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.055"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            125..667
                     /gene="zapC"
                     /locus_tag="ACHJWA_002692"
     CDS             125..667
                     /gene="zapC"
                     /locus_tag="ACHJWA_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFO4309262.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTNEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(664..1773)
                     /gene="ycbX"
                     /locus_tag="ACHJWA_002693"
     CDS             complement(664..1773)
                     /gene="ycbX"
                     /locus_tag="ACHJWA_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFO4309263.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDSSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PEAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILTTAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            2017..4125
                     /gene="rlmKL"
                     /locus_tag="ACHJWA_002694"
     CDS             2017..4125
                     /gene="rlmKL"
                     /locus_tag="ACHJWA_002694"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFO4309264.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKEPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            4137..6044
                     /gene="uup"
                     /locus_tag="ACHJWA_002695"
     CDS             4137..6044
                     /gene="uup"
                     /locus_tag="ACHJWA_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO4309265.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKNEEPAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            6174..7427
                     /gene="pqiA"
                     /locus_tag="ACHJWA_002696"
     CDS             6174..7427
                     /gene="pqiA"
                     /locus_tag="ACHJWA_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFO4309266.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCGTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            7432..9072
                     /gene="pqiB"
                     /locus_tag="ACHJWA_002697"
     CDS             7432..9072
                     /gene="pqiB"
                     /locus_tag="ACHJWA_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFO4309267.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     VLDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            9069..9632
                     /gene="pqiC"
                     /locus_tag="ACHJWA_002698"
     CDS             9069..9632
                     /gene="pqiC"
                     /locus_tag="ACHJWA_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFO4309268.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVAVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            9888..10055
                     /gene="rmf"
                     /locus_tag="ACHJWA_002699"
     CDS             9888..10055
                     /gene="rmf"
                     /locus_tag="ACHJWA_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFO4309269.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(10125..10643)
                     /gene="fabA"
                     /locus_tag="ACHJWA_002700"
     CDS             complement(10125..10643)
                     /gene="fabA"
                     /locus_tag="ACHJWA_002700"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFO4309270.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(10712..12472)
                     /gene="ycbZ"
                     /locus_tag="ACHJWA_002701"
     CDS             complement(10712..12472)
                     /gene="ycbZ"
                     /locus_tag="ACHJWA_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO4309271.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            12658..13110
                     /gene="matP"
                     /locus_tag="ACHJWA_002702"
     CDS             12658..13110
                     /gene="matP"
                     /locus_tag="ACHJWA_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFO4309272.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(13186..14226)
                     /gene="ompA"
                     /locus_tag="ACHJWA_002703"
     CDS             complement(13186..14226)
                     /gene="ompA"
                     /locus_tag="ACHJWA_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFO4309273.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(14583..15092)
                     /gene="sulA"
                     /locus_tag="ACHJWA_002704"
     CDS             complement(14583..15092)
                     /gene="sulA"
                     /locus_tag="ACHJWA_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFO4309274.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            15311..15940
                     /gene="sxy"
                     /locus_tag="ACHJWA_002705"
     CDS             15311..15940
                     /gene="sxy"
                     /locus_tag="ACHJWA_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFO4309275.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKYPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(15903..18065)
                     /gene="yccS"
                     /locus_tag="ACHJWA_002706"
     CDS             complement(15903..18065)
                     /gene="yccS"
                     /locus_tag="ACHJWA_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFO4309276.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(18075..18521)
                     /gene="yccF"
                     /locus_tag="ACHJWA_002707"
     CDS             complement(18075..18521)
                     /gene="yccF"
                     /locus_tag="ACHJWA_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFO4309277.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIALIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            18644..20698
                     /gene="helD"
                     /locus_tag="ACHJWA_002708"
     CDS             18644..20698
                     /gene="helD"
                     /locus_tag="ACHJWA_002708"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFO4309278.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVLIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(20730..21187)
                     /gene="mgsA"
                     /locus_tag="ACHJWA_002709"
                     /pseudo
     CDS             complement(20730..21187)
                     /gene="mgsA"
                     /locus_tag="ACHJWA_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
     gene            complement(21283..21945)
                     /gene="yccT"
                     /locus_tag="ACHJWA_002710"
     CDS             complement(21283..21945)
                     /gene="yccT"
                     /locus_tag="ACHJWA_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFO4309279.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            22118..22531
                     /gene="yccU"
                     /locus_tag="ACHJWA_002711"
     CDS             22118..22531
                     /gene="yccU"
                     /locus_tag="ACHJWA_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFO4309280.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(22576..22893)
                     /gene="hspQ"
                     /locus_tag="ACHJWA_002712"
     CDS             complement(22576..22893)
                     /gene="hspQ"
                     /locus_tag="ACHJWA_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFO4309281.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(22951..24141)
                     /gene="rlmI"
                     /locus_tag="ACHJWA_002713"
     CDS             complement(22951..24141)
                     /gene="rlmI"
                     /locus_tag="ACHJWA_002713"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFO4309282.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            24236..24514
                     /gene="yccX"
                     /locus_tag="ACHJWA_002714"
     CDS             24236..24514
                     /gene="yccX"
                     /locus_tag="ACHJWA_002714"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFO4309283.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(24511..24840)
                     /gene="tusE"
                     /locus_tag="ACHJWA_002715"
     CDS             complement(24511..24840)
                     /gene="tusE"
                     /locus_tag="ACHJWA_002715"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFO4309284.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(24931..25590)
                     /gene="yccA"
                     /locus_tag="ACHJWA_002716"
     CDS             complement(24931..25590)
                     /gene="yccA"
                     /locus_tag="ACHJWA_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFO4309285.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKMANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(25998..27017)
                     /locus_tag="ACHJWA_002717"
     CDS             complement(25998..27017)
                     /locus_tag="ACHJWA_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4309286.1"
                     /translation="MGRPRKNKKDNVLPPRVRSNGYSYVWKPEGSTRSIGLGRVRKTS
                     VAKVWQNYELEKAKLHNIMTVAKLWHMFMDSPAFTELAPRTQKDYRQHQKALLMVFGK
                     VLADNVKTEQVRIFMDKRGLESKTQANHELASLSRVYGWGYERGYVKNNPCKGVRKFS
                     LKARTVYITDEQYAAIYAEAIPQLRIAMEISYLCAARLGDVLELKWQDIMDKGIYIEQ
                     NKTGTKQIKEWSPRLRTAIQLARNVSSCTCEYVINTTKGGKVIAKTLNNWWNQAKRAA
                     EQKVGVPFGCNFHDIKAKGISDYEGSSRDKQIFSGHKTENQVLIYDRKTKITPTLDLP
                     LVVSK"
     gene            complement(26995..27237)
                     /locus_tag="ACHJWA_002718"
     CDS             complement(26995..27237)
                     /locus_tag="ACHJWA_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFO4309287.1"
                     /translation="MYELTLSPAEIKEITKYERYTKQQHQLRLHGIPFVIGPKNEPIV
                     LRRDIPHGLTTMPKTSELVSAEPDFEALNNGKTKKK"
BASE COUNT         6943 a         6656 c         7147 g         6624 t
ORIGIN      
        1 ccctggttgt tttatattca ttgctgttgc ttatttagac attttgtact tttattgttg
       61 aggttattaa gcgaagcgac aatggattgt gttgctgcgg ttttataggg taggggagag
      121 gcagatgcga attaaaccag acgataactg gcgttggtat tacgatgaag agcacgatcg
      181 tatgatgctc gatttagcca atggtatgct atttcgctca cgttttgcgc gcaaaatgtt
      241 gactccagat gccttttctc ccgcaggctt ttgcgttgac gacgccgcgc tctatttctc
      301 ttttgaagaa aagtgccgcg attttaattt atctaaagaa caaaaagccg aactggtgct
      361 gaatgcactg gtagcaattc gctatctcaa accgcaaatg ccgaaaagct ggcattttgt
      421 ttcccatggt gaaatgtggg tgcccatgcc gggcgatgcg gcctgcgttt ggttgagcga
      481 cactaacgag caagtgaatt tactggtcgt tgaatctggc gagaatgccg cgctatgcct
      541 gctggcacaa ccctgcgttg ttattgcggg tcgcgcgatg cagttgggcg acgccattaa
      601 aatcatgaac gacaggctga aaccgcaggt taatgttgac agcttcagcc tcgaacaggc
      661 agtctaacgc gccaacttaa gtgcagtctt cggtacacag ctacagcaaa gaatggtgcc
      721 atcatcgccc attgctgatt ttttcagcgg cgtgacttcg ccttctaaaa gctgaacacg
      781 gcaacttcca caaatgcccg cgcggcaaga ataagggata cgaattccct gattttctaa
      841 ttgctccagc aacacctgtt ggttatttcc acgaaatgcc tgtccctgcc agtcaatatc
      901 tacattggcg tccggttgtt gcgtgatgtt ggcagtatca tcagcggcag ctgcgccgta
      961 aatttttgcc ggagccgttg tcagaatttc cacctcatcg ccaacgcgaa tcacgccgct
     1021 attacgggca attaaattct gaccaaaatc gacatcgccg ttatcctggg cagtgcggaa
     1081 agattgcaat gtttttaatg gttcgcctgc cggatgtttt tgcccttttt ctgggctgac
     1141 ggtggtgaaa atacagcggc tacaaggttt aaccacatca aacaccacat caccaatgcg
     1201 aatcactttc caactatctt cttcccaggc tgacgcgccg gaaaccacca gattggggcg
     1261 gaattgctcc atttttacac tggccggaca acgttgttgg agatcacgta acgaggcttc
     1321 gttagcaaga aggtaaggat agccatcagc aaatgacagg ggtacagtgt tgtggcgttt
     1381 cacgcgccgg gtcatttgtg gccccaccca gcgtaattgc acttcgcggg agaaaaatcc
     1441 acttaaccat ttgttgatcg cttctggcgc aattcgcgcg gtaaaatgtg tgccccaaac
     1501 ttcggttggt gcgtcttgtg tggcgaaatc agcaaaacga acatatgcgc tactgctatc
     1561 tggtgcggtg agatgcaagc catcatgcac gggtgaagga gtaaatcgta ccatctgggg
     1621 aaactggcga gcggtgataa acgtaccgtc aggttccgtg atcataaaga tgcgatcgaa
     1681 ggccagacca ctgacatctg ccagagcatg tgtaagacca atgccgcgca tcgatttaac
     1741 aggatgaata aaaagccggg ttaatgtcgc cacggtgcgg tcctcaaatg aaaataagcc
     1801 ctcaacttta tgacatgacg cgcttattag ctataatgcg caacaatttt cttagcgcct
     1861 gtcccattag gctattttat ttgtcagttt ggccctgggt tgtgctcgaa atcctcacgt
     1921 acttgtgtac gctccggttt ctccgcgctg tccatgtcca gactgactgc aacaattacg
     1981 cctactgcgg taggctctta gagtaaaagt gacgatatga attctctgtt tgccagtacg
     2041 gcccgtgggc tggaagagct gttaaaaact gaactggaaa acctgggggc cgttgaatgc
     2101 caggtggttc agggtggggt ccatttcaag ggcgacacac ggcttgttta ccagagcctg
     2161 atgtggagcc gcctggcctc gcgtattatg ttgccgctgg gcgagtgtaa ggtttacagc
     2221 gatttagacc tctatctcgg tgttcaggcg atcaactgga cagagatgtt taatcctggc
     2281 gcgaccttcg ctgtccactt cagtggtttg aatgacacta tccgcaacag tcagtacggc
     2341 gcgatgaaag tgaaagacgc gatcgtcgat gctttcacgc ggaaaaatct gccgcgtcca
     2401 aatgttgatc gcgatgcgcc ggatatccgc gttaacgtct ggctgcataa agaaaccgcc
     2461 agtatcgccc ttgatctcag tggtgatggt ttacatctgc gtggctatcg cgatcgtgct
     2521 ggtattgcgc cgatcaaaga aaccctggca gccgctattg tgatgcgatc cggctggcag
     2581 ccaggaacac cgttgctcga tccgatgtgt ggttccggta cgttgctgat tgaagcggcg
     2641 atgctggcga ccgatcgcgc accaggcttg caccgtgggc gttggggctt tagcggctgg
     2701 gcgcagcatg atgaagctat ctggcaggaa gtgaaagcgg aagcgcaaac tcgcgcccgt
     2761 aaaggcctgg ctgagtatag ctctcatttt tacggttcgg acagcgacgc tcgggtgatt
     2821 caacgtgcac gcactaacgc ccgtcttgcg gggattggtg aactgatcac ctttgaggtg
     2881 aaagatgtcg cgcaactgac caatccgctg ccgaaagagc cgtacggcac agtgttaagc
     2941 aacccgccat acggggaacg tctggacagc gaaccggcgc tgattgcgct gcatagcctg
     3001 ctgggtcgga tcatgaaaaa ccagtttggt ggctggaatc tctctttgtt tagtgcctcg
     3061 ccggatctgc taagctgctt gcagctgcgt gcagacaaac agtacaaggc gaaaaacggc
     3121 ccgctggact gcgtacagaa aaattaccac gttgccgaaa gcactccaga cagcaaaccg
     3181 gcgatggtag cggaagacta cgccaaccgc ctgcgtaaga acctcaaaaa attcgagaag
     3241 tgggctcgtc aggaagggat tgaatgttac cgcctgtatg acgccgatct gccggaatat
     3301 aacgttgccg ttgaccgtta tgccgactgg gtggtggtgc aggagtatgc gccgccaaaa
     3361 actattgatg cgcacaaagc gcgtcagcgt ctgttcgata ttatcgctgc aaccatttcg
     3421 gtactgggga ttgcgccaaa caaactggtg ctgaaaaccc gtgaacgtca gaagggcaaa
     3481 aatcagtacc agaaactggg cgagaagggc gagtttctgg aagttaccga atataacgct
     3541 cacttgtggg tgaacctgac ggattatctc gatactggtc tgttcctcga tcaccgtatc
     3601 gcccgtcgta tgctcggcca gatgagcaaa ggcaaagatt tcctcaacct gttctcttat
     3661 accggcagcg ccaccgtgca tgcgggatta ggcggtgcac gcagcaccac caccgtggat
     3721 atgtcgcgta cttatctgga gtgggcagaa cgcaacctgc gtctgaatgg cctaaccggg
     3781 cgtgcgcatc gcctgattca ggccgattgc ctggcgtggc tgcgtgaggc aaatgaacag
     3841 ttcgatctga tctttatcga tccgccaacc ttctctaact caaaacgaat ggaagatgcg
     3901 tttgatgttc agcgcgatca tctggcgctg atgaaagatt tgaaacgtct gctgcgtgca
     3961 ggtgggacga tcatgttctc gaacaacaaa cgtggcttcc gtatggatct cgacggcctg
     4021 gcaaaactgg gactgaaagc acaagaaatt acgcaaaaaa cgctctccca ggatttcgcc
     4081 cgtaaccgcc agatccacaa ctgctggctg attaccgcag cctgaaagga atagtaatgt
     4141 cattaatcag tatgcatggc gcatggctgt cgttcagcga cgcgccgctt ctcgataacg
     4201 cagaactgca tatcgaagat aacgaacgtg tttgtctggt gggccgcaac ggcgcaggca
     4261 aatcgacgtt aatgaaaatc ctcaaccgtg aacaagggct ggatgacggt cgcattattt
     4321 acgagcagga tttgattgta gcgcgtctgc aacaggatcc gccgcgtaac gttgagggta
     4381 gtgtttatga tttcgttgcc gaaggcattg aagaacaagc ggaatacctg aaacgctatc
     4441 acgatatttc gcgcctggtg atgaacgacc cgagcgagaa aaatctcaac gaactggcga
     4501 aggttcagga acagctggat caccacaacc tgtggcagct ggaaaaccgc atcaacgaag
     4561 tgctggcgca actggggtta gatcctaacg ttgcgctgtc gtcgctttcc ggcggctggt
     4621 tgcgtaaagc ggcattagga cgcgcgctgg tgagcaatcc gcgcgtattg ctgctggacg
     4681 aaccgactaa ccacctggat atcgaaacca tcgactggct ggaagggttc ctcaaaacct
     4741 tcaatgggac gattattttc atctcccacg accgttcgtt tatccgcaat atggcgacgc
     4801 gcattgttga tctcgatcgc ggcaagctgg tgacctatcc gggcaattac gatcagtacc
     4861 tgctggaaaa agaagaagcc ctgcgcgtgg aagaactaca aaatgccgag tttgatcgca
     4921 aactggcgca ggaagaagtg tggattcgcc aggggatcaa agcacgccgt acccgtaatg
     4981 aaggccgcgt acgcgccctg aaagcgatgc gtcgcgagcg tggcgaacgt cgcgaagtga
     5041 tgggtaccgc aaagatgcag gtggaagagg ccagccgctc cggtaagatc gttttcgaaa
     5101 tggaagacgt ttgctaccag gttgacggaa agcaactggt gaaagatttt tctgcccagg
     5161 ttctacgtgg cgacaaaatt gccctgattg ggccgaatgg ctgcggcaaa accacgctgc
     5221 tgaaactgat gctcggtcag cttcaagcgg acagcgggcg tattcacgtt ggcaccaaac
     5281 tggaagtggc ttatttcgat cagcaccgcg cggaactgga tcccgataaa acggtgatgg
     5341 ataaccttgc cgaaggtaag caagaggtga tggttaacgg caagccacgc cacgttttgg
     5401 gctatttgca ggacttcctg ttccatccga aacgggcgat gacgccagta cgtgcgcttt
     5461 ctggcggtga gcggaaccgc ttgctgctgg cgcgtttgtt cctcaaacca agcaacttat
     5521 tgattcttga cgaaccgacc aacgatcttg atgtcgaaac gctggaactg ctggaagaac
     5581 tgatcgacag ctaccagggc acggtattgc tggtaagcca cgatcgtcag tttgtcgata
     5641 acaccgttac agaatgctgg atcttcgaag gcggcggtaa aattggtcgt tatgtcggcg
     5701 gttatcatga tgcccgtggt cagcaagagc agtatgtggc gctcaaacag cctgcggtga
     5761 aaaaaaacga agaacccgcc gcgccaaaag cagaaactgt aaaacgcagc agtagcaaac
     5821 taagctataa attgcagcgc gaactggagc agctaccgca attgctcgaa gatctggagg
     5881 cgaagctgga agccctacag acgcaggtgg cggatgcttc cttcttcagt cagccgcatg
     5941 agcagacaca aaaagtgctc gccgatatgg ctgctgcaga gcaggagctg gagcaagcct
     6001 ttgaacgctg ggagtatctt gaagcgttaa aaaatggtgg ctgatcgcaa aatagtcgat
     6061 ttaaacagag acagctgaca acggtaaaat tgcctgatga actgggttta tcaggcatga
     6121 aaacaaacac tttgttagtc atctgtaaaa tagcgcatca ttaaggagta ccaatgtgcg
     6181 aacatcatca tgccgcgaag cacatcctgt gctcgcagtg tgacatgctg gtggcgttac
     6241 cgcgccttga gcatggtcag aaagcggcat gtccccggtg tggcacaacg ttaaccgtgg
     6301 cgtgggatgc cccccggcag cgtccgaccg cctatgcgtt ggctgcactg ttcatgctgt
     6361 tgctgtccaa cttgtttcct tttgtgaata tgaacgttgc aggagttacc agtgaaatta
     6421 cattactgga aattcccggc gtgctttttt ctgaggacta cgccagcctc ggcacctttt
     6481 tcctgttgtt tgtgcaactg gttcccgcgt tttgtctgat aaccattctg ttactggtga
     6541 atcgcgcgga attaccggtc cgtttaaaag agcaactggc acgggtgctt tttcaactca
     6601 aaacctgggg aatggcggag attttcctcg cgggtgtgct ggtcagtttc gttaaactga
     6661 tggcttacgg cagcattggc gtaggcagca gctttctccc ctggtgttta ttttgtgtcc
     6721 tgcaactgcg cgcttttcag tgcgttgatc gtcgctggtt atgggacgat atcgccccga
     6781 tgccagaact gcgccagccg ctaaaaccag gcgtcacggg gatacgtcag gggctgcgtt
     6841 cgtgctcctg ttgtacggca atccttcctg ctgatgaacc cgtgtgcccg cgctgtggta
     6901 ctaaagggta cgttcgacgt agaaacagcc tgcaatggac actcgcgctg cttgttacgt
     6961 ccatcatgct gtatcttccg gctaatattt tgcccatcat ggtgacggat ttattaggct
     7021 cgaagatgcc atcgacgatt ctcgctgggg tcatcctgtt atggagcgaa ggctcttatc
     7081 ccgtcgctgc ggtgatcttt ctggccagta ttatggtgcc aacgttaaag atgatcgcca
     7141 tcgcgtggct gtgttgggat gccaaagggc atggcaagcg cgacagtgaa agaatgcatt
     7201 tgatttatga agttgttgag tttgtaggcc gctggtcgat gattgacgtt ttcgttatcg
     7261 cggtgctctc ggcgctggtg cgtatgggag gtttaatgag tatttatccg gcaatgggtg
     7321 cattaatgtt tgctttagtc gtcataatga caatgttttc tgctatgacg tttgacccgc
     7381 gtttgtcgtg ggatcgtcaa cctgaatcag agcatgagga gtcctgacag tatggaatct
     7441 aataatgggg aagccaaaat ccagaaagtg aagaactggt ctcccgtgtg gatatttcct
     7501 atcgtcacgg cgctcattgg ggcctgggtt cttttttatc attacagcca tcagggaccg
     7561 gaagtgaccc tgatcaccgc gaatgcggaa ggaattgaag gtggcaaaac caccattaaa
     7621 agccgtagcg ttgacgtcgg cgtggttgaa agcgccacac tggctgatga tttgacacac
     7681 gttgaaatca aagcgcggct gaattccggt atggaaaaat tgctgcataa agataccgtc
     7741 ttttgggtgg tgaaaccgca gattggtcga gaagggatta gcggtctggg aaccctgctg
     7801 tctggggttt atatcgaact gcagccaggc gcgaaaggca gcaaaatgga taaatacgat
     7861 ttgctggact cgccaccgtt ggccccgcct gatgcgaaag gtatccgtgt ggttctcgat
     7921 agcaaaaaag ccgggcagct ctcgccagga gatccggtgc tgttccgtgg ctatcgggta
     7981 ggttcggttg aaaccagcac cttcgatacg caaaaacgca atatcagtta tcaactgttc
     8041 atcaatgcac cttatgaccg actggtgacc agcaatgttc gcttctggaa agatagtggc
     8101 attgcggttg atctgacgtc agcgggaatg cgtgtggaga tgggctcatt gacaacgctg
     8161 ctgagtggcg gtgtcagctt tgatgtgccg gaaggtctgg atttagggca gccagtggca
     8221 ccgaaaacag ctttcgtttt gtatgatgat cagaagagca ttcaggattc gttgtacacc
     8281 gatcacattg attatctgat gttctttaaa gattcggtac gcggtctgca accgggagct
     8341 ccggtagaat tccggggtat tcgcctcggt accgtaagca aagtgccatt ctttgcgccg
     8401 aatatgcgtc agacatttaa cgatgattac cgtattccgg tgctgattcg tatcgagcca
     8461 gagcggctga aaatgcagct tggcgaaaat gcggatgttg ttgagcacct tggcgaattg
     8521 ttgaaacgtg gtttacgcgg atcgctgaaa accggaaacc tggtcactgg cgcactgtat
     8581 gttgatctcg atttctatcc aaatacgcct gcaataaccg gtattcgtga atttaatggt
     8641 tatcagatta tcccgaccgt tagcggcggc ctggcgcaaa tccagcaacg actgatggaa
     8701 gcgttggata agatcaacaa actgccattg aatccgatga ttgaacaggc aaccagtacg
     8761 ctttctgaaa gtcagcgcac aatgaaaaac ctgcaaacga cgctggatag catgaacaag
     8821 atcctcgcca gccagtcgat gcagcagttg ccgacggata tgcagtcaac gttgcgtgaa
     8881 ttgaatcgca gcatgcaggg cttccagccc ggctccgcag cctacaacaa gatggtggcg
     8941 gatatgcagc gccttgatca ggtgttgcga gaactgcaac cggtgctgaa aacgctcaat
     9001 gagaagagta acgcgctggt atttgaagcg aaggacaaaa aagatccaga gccgaagagg
     9061 gcgaaacaat gaaaaagtgg ctagtgacga ttgcagcact gtggctggcc ggatgcagct
     9121 ccggcgaaat taataaaaac tattaccagt tacctgtggt gcagagcggt acacaaagta
     9181 ccgccagcca gggcaatcgc ctgttatggg tagagcaggt cgctgtacct gactatctgg
     9241 cggggaatgg tgtggtttat caaaccagtg atgtgaagta tgtgattgcc aacaacaact
     9301 tgtgggccag cccgttggat caacagttgc gcaacaccct ggtagccaac ctgagcacgc
     9361 aactgcccgg ctgggtggtt gcctcccagc ctctgggaag cgcccaggac acgctcaatg
     9421 ttaccgtaac ggagtttaac ggtcgctatg atggcaaggt cattgtcagt ggtgagtggc
     9481 tgttgaacca ccagggacaa ctgatcaaac gtccgttccg tctggaagga gtgcaaactc
     9541 aggatggtta cgatgagatg gttaaagtgc tggccggtgt ctggagtcag gaagccgctt
     9601 ctattgcaca agagataaag cgtctacctt aattataaag atttgtaaat ataaccgtct
     9661 ccggtatgtt gcctgaggcg gtttttttgt ttctaacgtg cggaaaaatt tgttcctctt
     9721 cacatttttt gtacaaccga catgcccgtg tagctcacaa atatgacagt ggcgtgaatt
     9781 ttgcgcattg acggcagtta tgattcgcgg tattgcttaa ctgtgattgc acatttagta
     9841 atcactgttt tcttttccac cagaaaccag tatgagggaa acgaggcatg aagagacaaa
     9901 aacgagatcg cctggaacgg gcacatcaac gtggttatca ggccggcatc gccggacgct
     9961 caaaagaaat gtgtccctat cagacgctga atcaaaggtc acaatggctg ggaggctggc
    10021 gagaagccat ggcggacagg gtagtaatgg cctgattctg tctctttaaa aagaaacctc
    10081 cgcattgcgg aggtttcgcc ttttgatact ctgtctgatt ataatcagaa ggcagacgta
    10141 tcctggaaca gaccgacttt caggtcgctg gcggtataga tcagacgacc atcaaccagc
    10201 acttcgccat ccgccaggcc cataatcaga cgacggttaa caatgcgttt aaagtgaata
    10261 cggtaggtca cttttttcgc tgtcggcagt acctgaccag tgaatttcac ttcgccaacg
    10321 cccagcgcgc ggcctttacc ttcgccgccc agccagccga ggtagaaccc taccagctgc
    10381 cacattgcgt ccaggcccag gcatcccggc ataaccggat cgccaataaa gtggcatccg
    10441 aagaaccaca gatccggatt gatatccagt tctgcttcaa catacccttt gtcgaagtta
    10501 ccacccgttt cggtcatttt gaccacacgg tccatcatca gcatgttcgg tgctggcaat
    10561 tgcgggcctt tagcgccaaa cagttcaccg cgaccagagg caagaaggtc ttcttttgta
    10621 taggattcgc gtttatctac catgttctct gtaagcctta ttttattgaa gcacgcagga
    10681 tagctaacac gtgtacgctg aacaagtccg atcagttcgg aataaaccag ttcagccaac
    10741 gtaatggcca tggaaaacgg tgacgtcctt cctgttgcga tgcttgcgcg atacgttcct
    10801 ggatggtttg catcagcgtc gtttggcctt cgccatccca caccagattt aataataacg
    10861 gcagtgcgtc agtcacatcg tctactgccc agatggtgaa tttgccttct tctaccgctt
    10921 tcaccagttc actgtgaaga cttaaatggc gaacgttagc tgtggggata atgacacctt
    10981 gtttcccggt taactcacgt tgctggcaaa tagcaaagaa gccttcgatt ttctcattta
    11041 aaccaccgac cggctgggcg cgaccgaact gatcgactga acctgtgata gcgatactct
    11101 gattcaccgg cacatcggcg agggcgctta tcagggcgca gagttcagcc atcgaggcac
    11161 tatctccatc aacttcactg tatgactgct caaatgtcag cgatgctgag aaggggatct
    11221 gttgctcaag ctgtagttcc gacatcagga acgcttgcat gatcatcatc cctttcgcat
    11281 ggatattgcc gccaagctcc gctttgcgtt cgatgtcggt gaattcacca tcgccaatat
    11341 gcacaacgca gctaatgcga gaaggttcgc caaaagcgcg tggatgaccc ggaaattcaa
    11401 tgaccgaaag ggcgttaatt tgcccgatgc gttcgccttc ggtttcaatc aggatttgct
    11461 cctgaaggat ctcatcctgc atacgttcag cgaggaaacc ttcgcgccat tcacgctgct
    11521 gcagcattaa gtttagctgc tcgccggaga aggtgtcgcc atcacacagg gaggcgacct
    11581 ctttacactg gcggaggatc cactgcgggc taagcggaag tgtttcttgt tcaccggtgt
    11641 agcgtgctgc ttcgcggata agtaccggcc aggcatccgc tcccggggca ggtaagtgat
    11701 tatgtctggc ggtaaatgtc acccagcgac accactgggt tactgactcc gcatcgacaa
    11761 tctgcagagt atcttcaaat tcgctataaa tagcctgctc tgaaagctct ggctccatct
    11821 cctggaaatc agccaatgat tcgcgttcgc ctaccagaat gactttcagc ttcaatggca
    11881 tcgaaggcac agagacgggg agagggcgcg actcatcaaa cgcaacccag tcaaaacgct
    11941 cgcggttaac gatatttttc agccgcatcc acagcagagg ttgcgccagc agtgtacgca
    12001 aagagatgat gagaataccg ccatttgcct gatgcaccag accaggctgc agggtaatgt
    12061 cgccattaaa ctggcgcagg cagccaaaga gttgctccgc ttctacccag tcggcagcga
    12121 caacttgcgt taaagtcgca aaattatcat ctgcactcac tgcgtgacgt aagcggatgg
    12181 agtggccgga aacctcatag tgaccgccca ccagttggcc tgcatcgctt tgtagcgtac
    12241 gcgcggcatt ggcaataaga ttcagatact cagactcttc cggggccttc gccagcataa
    12301 aagaggagga tgctcgcgta tgcagcaact gctccagcgc aaattgcagc cgcggttgag
    12361 tatcactgaa taaaggatcg ttttcgtcaa tcaaatgtgg ctgagcaaat atttcctgat
    12421 agctatcggt atcaggaacc aggtcacgcc atgcaagttt cgtaatggtc aaagttgatg
    12481 ttttttagtc tgttgtcaaa gccgcgatta taccgtaacc ggcactacag cacacgtaga
    12541 aagcaccgac aatactcctg gcatgggcgt taaagctcac aggatggaga ttcttttctt
    12601 cactggccta aaaagctgat attctgtaaa gagttacacg gtaacattga gatcgctatg
    12661 aaatatcaac aacttgaaaa tcttgaaagc ggttggaaat ggaagtatct ggtgaagaag
    12721 catcgcgaag gggagttaat cacccgttac atagaagcca gtgccgccca ggaagccgtc
    12781 gatgtgttgc tctcgctgga aaatgaaccc gtgctggtaa atggctggat tgacaagcat
    12841 atgaatccgg aactggtcaa tcgcatgaag caaaccattc gggcaagacg aaagcgccat
    12901 tttaatgcgg aacaccagca tacgcgcaaa aaatcgatcg atctggaatt tattgtctgg
    12961 caacgtctgg ctggtcttgc gcagcgtcgc ggtaaaacgc tttctgaaac gattgttcag
    13021 ctgattgaag atgcggaaaa caaagagaaa tacgcgaata aaatgtcttc tctgaagcag
    13081 gatctgcagg cattgctggg taaggaataa ctgacgaaag tcagttcaat ttactaaagg
    13141 caaaaaaaac cccgcagcag cggggttttt ctaccagacg agaacttaag cttgcggctg
    13201 agttacaacg tctttgatac ctttaacttc gatctctacg cgacgatccg gagccaggca
    13261 gtcgatcagt gcagcacgct gtttcacgtt gtcacaggtg ttgccagtaa ccgggttgga
    13321 ttcgcccata ccacgtgcgg agatcttgtc tgccgggata cctttggaga tcaggtaatc
    13381 aacaacagac tgagcacggc gctcggacag accctggttg taagcgtcag aaccgatgcg
    13441 gtcggtgtaa cccagaacaa ctacggaacc gtctttcgga tccaggttgc tcagctggct
    13501 gtacagctga tccagagcag cctgaccttc cggtttcagg gttgctttgt tgaagttgaa
    13561 cagaacgtca gacttcagag tgaagtgctt ggtctgtact tccggtgccg gagctggagc
    13621 cggagcaact actggagctg cttcgccctg accgaaacgg taggaaacac ccaggctcag
    13681 catgccgttg tccggacgag tgccgatggt gtgtgcgtca ccgatgttgt tggtccactg
    13741 gtattccaga cgggtagcga tttcaggagt gatcgcgtac tcaacaccgc cagcgaagac
    13801 cggagaaacg ccggtgtcgt ggtttttacc ataaacgttg gatttagtgt ctgcacgcca
    13861 taccatacca cccagacgag tgtagatgtc caggtcgtca gtgattgggt aacccagttt
    13921 agcggtcagt tgaacgccct gagctttgta tgcaccgttt tcaacgctgc ctttgtacgg
    13981 catacgacct aaccagtcgt aacccatttc aaagccaaca tacgggttaa cctggtaacc
    14041 accaaaagca ccagcgccca gttggttttc atgggtcggg ccattgttgt tgatgaaacc
    14101 agtgtcatgg tactgggacc agcccagttt agcaccagtg taccaggtgt tatctttcgg
    14161 agcggcctgc gctacggtag cgaaaccagc cagtgccact gcaatcgcga tagctgtctt
    14221 tttcattttt tgcgcctcgt tatcatccaa aatacgccat gaatatctcc aacgagataa
    14281 cacggttaaa tccttcaccg ggggatctgc tcaatattaa ctctaccgat atcttcggct
    14341 tatgccgagc acccctggcg atgtaaagtc tacaacgtag ttgaaaactt acaagtgtga
    14401 actccgtcag gcatatgaaa aaaaagtctt gtataaggta tgtttaatct tttttgtcag
    14461 cgacaattta cagaagagaa tcgcggaaac cgcttcagac aagcctccgc aaggaaaatt
    14521 agtcacgact gaaagcattg gctgggcgac aaaaaaagtt ccaggattaa tcctaaattt
    14581 acttaatgat acaaattaga gtgaattttt agcccggaaa gttgtctcgt ggcgtgagag
    14641 gatgcgctta ccggacgcat aataaacccc atagcgttac cttcatttgc cgcatcaaca
    14701 agttcagcat gctcttcttc agtcaaatca tctgccaacc aaccgatcac cacactgtaa
    14761 ttgcccgtgc gtaaagcgcg aaccattgac tccacagtgt ggcaagggga gagctggcta
    14821 atctgcatta ctttcgttaa gggtagccca gatgcctgaa cccattcccg actcagtttt
    14881 tgttgcggtg ttaaccagag ttgccagcgc gattgctgac cgagttgctg taacaatggc
    14941 aacagtagaa gttgcgtcat catgggctga tcttcgcgat agacaacttc actgataagc
    15001 ccggctgtag tgttttccgt agagacacgc gcaattttac ttgctgcgga tgagaacgac
    15061 gaagaacgat gtgcatagcc tgaagtgtac ataatcaatc cagcccctgt gagttactgt
    15121 atggatgtac agtacatcca gtgacaacaa agatcaaccc tattttcgga aagagcctcg
    15181 caaattttgt cgttggtgac gggaaaacat aaattaatct tgccgcttaa gaataagttg
    15241 cctattttcg tagttaacgg atccgttaat gtgaatcatt cttttatgtt atgattttaa
    15301 aaggaatttt atgaaaagcc tctcctataa gcggatctat aaatcacaag aatacctggc
    15361 aacgttgggc acaattgaat accgatcatt gtttggcagt tacagcctga ccgttgacga
    15421 cacggtgttt gcgatggttt ctgatggtga gttgtatctt cgggcttgtg agcaaagtgc
    15481 acagtactgt gtaaaatatc cgcctgtctg gctgacatat aaaaagtgtg gccgatccgt
    15541 taccctcaat tactatcggg ttgatgaaag tctatggcga aatcaactga agctggtgcg
    15601 tctgtcgaaa tattctctcg atgcagcgct gaaagagaaa agcacgcgca atacccggga
    15661 aagactgaaa gatttgccca atatgtcttt tcatctggaa gcgattctcg gggaggtggg
    15721 gattaaggat gtacgggcgt tacgtatact tggggcaaaa atgtgttggt tgcgactgcg
    15781 gcagcaaaac agtctggtga cagaaaagat tctgtttatg cttgaaggtg ccattatcgg
    15841 cattcatgaa gctgcgctcc cggtggcacg ccgccaggag cttgcagaat gggctgactc
    15901 tcttacgccg aaacaggagt ttcctgcgga acttgagtaa tctggcgttg cagacgacca
    15961 atctcaggca gtaatgcaat caataatcca acttgttgta cgaccagagg ttctttgctg
    16021 tctgcccgtg gttcaagttg ctgcatccgc tgtttcaggc tagctaatgc ctcattgacg
    16081 cgttcttcat cagcaggttg atgatgtaac gcgtcatcaa catagcaaac tgcgtcatca
    16141 agaaacgcca gaatttcagg attagttaac tgctcccggt gagcaccgag ggctgagata
    16201 tagctggtaa acgtatggtt aaggcacagc aaccgaaacg cggcttcgcg aatttgcggg
    16261 gtaacgttcg gctcgctgga catatttgat accaccgacg ccagctcagc atcacggttg
    16321 tgtgcatcgc ggcgggcaat acgatacgcc agacggttat cacgcccctg atggtattgc
    16381 tccagtatgg catcgagata ccgacagttg gcctctgtgg cgcgttcgag catgcgcggc
    16441 agattgcgaa actgccagtc aggccagatg tagctcactg ccgcccacgc aatggcacaa
    16501 ccaatcagcg tatcgattac gcgaggtaac gctacttcaa aaccttcacc cagtaagtta
    16561 aaacacagta gcaccaaaag tgtgatgaac atcgttgcat gagcgtattg cacgttacgg
    16621 aaggcaaaaa agagcacgcc ggtaataacc agcagcacca gctgcccttc cagtgatggc
    16681 acaaaccaca gcacaggaat gccaatggcg atacctacca gcgtaccaat aatccttaac
    16741 ttcaggcggt ggcgcgtggc gttatagttt ggctggcaga caaacaaact tgtcagcaag
    16801 atccaatacc cgtgatgcat tccggttatc tgaatgatgg cgtagccgaa gcacaacacc
    16861 agcgacattc ttaccgcatg acggaagagg gcggattccg gcgtgaagtg acggctaaga
    16921 cgcagccaga tatcactcaa cccgtgcggg ctgtcatcag cgagctcatt ttcgtcatta
    16981 ttatggggta gtgcctgggc ctgttctgat tcaattgttg ccagttgggc atcaatggcg
    17041 cgtaaattgt tcagcaaaaa tcccagtgtt ttgagtaaat cggcgggtgc gccgttatcg
    17101 cgcatccgct ccagcgcagc atcaatatgc gtaaaagcgc gctcaaaatg cggatcatgt
    17161 tgataaggct gacgcaacaa aatacagcgt gacagttgct ggcacgcctg gccctgcatc
    17221 gacatcagcc gctgaaaacg gaacagcacg tcgctgtggc gaaaatgttc acgcaatgtt
    17281 tgatactgaa tatgagaaga gctggcacgc tcgtgaatat cctgtgcgac aaagtaataa
    17341 tgcagcgtgc gacgcgttcc ccgttgacca cgatcgccac gtaagcgggt cagcagcgag
    17401 agtttcgtct gattcaatgt cgccatcagc agaccgttgg cgagagccaa atcgtacagc
    17461 ggtgcctggc tttcatcttc aatatcagga tcaaacatgc gcgacttgag ctcaagataa
    17521 cgcgccagtt gttcatagca acgcgccagg ttgtcctgca gcgggcggac cgggaacagc
    17581 agatgaccaa taagtgtcag gacgttgtac cagacggcac cggccagcag atacatcggc
    17641 tgctgatacc agtgctcata cagtgatgtt cccaacatag tgtaaatggc gatcagcaat
    17701 gcaccgaagg caattgttgc atagcgttga cccagaccgc cgagcaaaat gaagccgctg
    17761 gtagagagcg ttaagccaat cgcaaatagc cagggccagg gaaacagcaa ttctactgag
    17821 gccgaggcga taaaaaagca gaacagcgta atgatgaggt tacgcaaacg tcccgccagt
    17881 cggtcatcga gatcggtcag cgccgctgcc accatcccca gcgttagcgg aatcgtcagt
    17941 tttacatcac ccagccacca cggaaacgct gtggttccac aaagcgcaat aaaaatacgc
    18001 gcgtaataca gccaggcgct gttccaggta tagcgtttga gcaaaggact tagcataaag
    18061 gccatattag tggattattc aaaacgacga cgtgcattgg cttcacgcgc agcttgcgct
    18121 gtttctaccg ataccacgcg acgaccaacc ggccatagtg caatagcggc aattttaaag
    18181 ttcgcaatgc cgacaggaat gccaatgatt gaaatacatt gtgcgatgcc cgttgcaatg
    18241 tgcatcaggc ataaccacca gccaaagaaa atcagccaga aaatattcaa taccgtaccg
    18301 ccagtattca gcagcacatt tttgccagcc gggttcagtt catcgacatg aatagcttca
    18361 ttgccataag gcaccagaga cagtttagtg atctcccagc aggatcgtgt cagcggtaag
    18421 gtaaaaatca gcgcaatact gaccagagtc gccaacagcc agcccagagt ggtggcaaat
    18481 ccgccaagca caaagttcag aatgttcaaa acggtacgca taaaacctcg ctttactgtg
    18541 gttttcagta atgggcggca attgtaacgt ttttttgggc tggagcacgt tttctctgac
    18601 ggttacactg ataagaaata atttcgtgtg gatctacaga gtcatggaac tgaaagcgac
    18661 aacgcttgga aaacgtctgg cacagcaccc ttacgatcgg gcggtgatcc tcaatgccgg
    18721 gattaaagtc tccggcgatc gccacgaata ccttattcct ttcaatcaat tactggcgat
    18781 tcactgtaag cgcggtctgg tatggggcga gctggaattt gtactgccgg acgaaaaagt
    18841 ggtgcgtctg cacggtaccg aatggggcga gacgcagcgt ttttaccatc atcttgatgc
    18901 tcactggcgg cggtggagtg gcgagatgag cgaaattgcg tctggtgttt tacgccagca
    18961 actggatttg attgccacgc gcactggaga aaataaatgg ctgacgcgtg agcaaacctc
    19021 tggcgttcag caacaaatcc gccaggcttt gtcggcgttg ccgttgccgg ttaaccgact
    19081 ggaagaattc gataactgcc gtgaggcgtg gcgtaaatgt caggcctggt tgaaagatat
    19141 tgaaagcgct cggttgcagc ataaccaggc gtataccgaa gccatgctta ccgagtatgc
    19201 ggattttttc cgccaggtcg agtcttcacc gctgaatccg gcgcaggccc gggcagtcgt
    19261 taatggcgag cattctctgt tagtgctggc aggtgcagga agcggaaaaa cgtcggtgct
    19321 ggtggcccgt gcaggctggt tgctggcgcg tggtgaagcg tcccctgagc aaattttatt
    19381 gctggcgttt ggtcgcaaag ccgctgaaga gatggacgag cgtattcgcg aacggctaca
    19441 taccgaagac attaccgcac gcacgtttca tgcgctggcg ctgcatatta ttcagcaggg
    19501 cagcaaaaaa gttccgatag tcagcaaact ggaaaatgat accgctgccc gtcatgaact
    19561 ctttattgct gagtggcgca agcaatgcag cgaaaagaaa gcgcaggcga agggctggcg
    19621 gcaatggctg acggaagaaa tgcagtggtc agtgccagaa ggtaacttct gggatgatga
    19681 aaaattacag cgtcgtcttg cctcacgcct cgatcgttgg gtaagtctga tgcggatgca
    19741 cggtggtgca caggcagaaa tgattgccag tgcacccgaa gagattcgcg atctgttcag
    19801 taaacgtatc aagttgatgg ccccgttatt aaaagcctgg aaaggtgcgc tgaaggcaga
    19861 aaacgctgtc gatttttcgg gccttattca tcaggcgatt gtgattctgg agaaaggtcg
    19921 ctttatcagc ccgtggaagc atattctggt tgatgaattt caggatatct cgccgcagcg
    19981 ggcagcgttg ttagcggcat tacgcaagca aaacagtcag acgacgttgt tcgccgttgg
    20041 tgatgactgg caggcgattt accgattcag cggtgcgcaa atgtcgctca ccaccgcttt
    20101 ccatgaaaac tttggtgaag gcgaacgctg tgatttagac acgacttacc gttttaacag
    20161 tcgtatcggt gaggtggcaa accggtttat tcagcagaac ccaggccagc tgaaaaagcc
    20221 gctaaacagc ttaaccaatg gagacaaaaa agccgtcacg ttattggatg agagtcaact
    20281 tgacgctttg ctggataagc tctctggtta tgccaaaccg gaagagcgca ttctgatcct
    20341 ggcgcgttac catcacatga ggcctgccag cctggaaaaa gcggcaacac gctggccgaa
    20401 gttgcaaatc gactttatga ccattcatgc cagcaaaggg caacaggcgg attacgtcct
    20461 catcgttggc ttgcaggagg gaagtgatgg ttttccggct gcggcgcggg agtcgattat
    20521 ggaagaggcg ctactgccac cggttgagga tttcccggac gctgaagaac ggcggttaat
    20581 gtacgtggcg ctgacccggg cacgtcatcg ggtatgggca ctgtttaaca aagagaatcc
    20641 ctctcccttt gtggaaatac tgaaaaatct ggatgtgccg gtggcgagaa aaccgtaaga
    20701 aacaggtggc gtttgccacc tgtgcaatat tacttcagac ggtccgcgag ataacgctga
    20761 taatcgggga tcagaatatc gaccgcgtcg ttgaaatgcg gcgactggat aatgaagtct
    20821 gccgttgcca cgttggtggc gaccggaatg ttccataccg tcgccagacg cagcaaggct
    20881 ttcacgtcag gatcgtgcgg cacggcattt agtggatccc agaagaaaat caatacatca
    20941 attttccctt ctgagatcaa tgcgccaacc tgctggtcac ccccattggg ccactcaaca
    21001 tcgcgttgac gttcatgccg gtcgcgcggg aaattaagtt accggtagtg cctgttgcat
    21061 acagtacgtg ttgttccagt aacggttgat gccgttccac ccagctcatc agcatttgtt
    21121 tgcagtgatc gtgtgccacc agcgcaatat gtttccgcgc aggtaaagtg cgagtcgtca
    21181 gttccataat gtacatccgt agttaacttt cctacagatt actgtaagca cttatcgctg
    21241 caagataaag accgaaaaag cctgcgcaca ggcacaaaaa tctcaggaag atggttgttt
    21301 ttccgcccat tgcaggaaag tatttcgcgt ttgtgggtca gccagtttaa accaatactt
    21361 cagccgttgt tctgtgagca cctgagactg cggtggaata gcatccagct cggaaacacc
    21421 cgaaagcagc gtactgtcat ctgccatcat cgtggcaaat tgcggtagtg aagcgcgttt
    21481 cgcggatttg ttatagcgtt caacctcgac ctcataatca atcgtttttg ccgttgaggt
    21541 aatggcgaga atatccagct ttaccggaat cggcgtcgca tcgccatcca acaattcaag
    21601 gcgcggcgcg gcatcaaaat ggttagcttc ccgctcattc tccaggcgag gcaggcgaaa
    21661 attgacctgg ttgatgagct gggtattaaa actgaccacc agcggtgggg agatatacag
    21721 ccgttcttca ctattggaaa gatgaatcgt cttctcaacg cgaaacacta actgatgcgg
    21781 tccattatcc agttcaatgc tgtccgcacc gcgcagcaga gagctggaaa ccttcttgcc
    21841 gtcgagtacc agaagatcga catcggttga aagccgcagc gtggtggcaa aaacggatac
    21901 cggcaaacat aatgcaatca aggtggtcac gatgccggtt ttcataggta gctcctgtca
    21961 aaagaccgcc cgaataataa tgtgtcaaaa tttttcttta atcatactta ctaacatata
    22021 gacatatttc ccgcttttgc tctcattcat tcgtattagc tgcatggttg gcatgtcggc
    22081 tttgtcgtac actttgtgaa acagtctgga ggaaataatg aaagaaaccg atattgcagg
    22141 cattttaacg tccactcaca ccattgcgct ggtgggggca agcgacaaac ccgatcgtcc
    22201 cagctatcgc gtgatgaaat atctactcga ccaggggtat cacgtcattc cggtttcgcc
    22261 aaaagttgcc ggcaaaacgc tgctgggcca gcaggggtac ggcacgctgg cagacgttcc
    22321 ggaaaaagtc gatatggtcg atgttttccg taattcagaa gcggcgtggg gcgtagcgca
    22381 ggaagctatc gccattggcg cgaaaaccct ctggatgcaa ctgggggtga ttaatgagca
    22441 agcggcagta ctggcacggg atgccgggtt gaatgtggtg atggatcgct gcccggctat
    22501 tgagatccct cgcctgggcc tggccaaata aaaaatcccc ggaaggcaaa aaccttccgg
    22561 ggatctgttc agggattagt tacgcagacg cggggcctgg agttgtttgc ggatggtctg
    22621 cgccagctca tccatggatg gctgttcagg atgttcatcc tgcaattcgc tacttagctg
    22681 ggcttcagcc aggtaggtat gaaccggtag gccgttatcg tcctccatca ccacgtgata
    22741 ccacggagcg gcgcgaagct cgtcattcac cgccagctca tcaggcgacg gttcagagag
    22801 cgaataaacc gggtcgatat ccacgaccac tccgagataa cctaacaggg aatggcggac
    22861 ctgctggccg ataccgaatt tgctggcaat catagtcacc tcccgggaaa tctttacctt
    22921 tgatataagg gtaaaattcc acatttcaag ttacatgacg cgacaggcaa accctttcag
    22981 atatagccct tccggatagg tagcgatcac cggatgatcg gccgcctgac ggaactgctc
    23041 tataaattgt acatcacgac cggcatcaat tgcggcatcc gcgatgattt tctgaaataa
    23101 atcgctggtc atcagaccgg aacaggagaa agtcaggaga ataccgcctt cattcagcag
    23161 ctgaatcgcc agcatgttga tatctttata gccacgacac gcgcccatca actggctttt
    23221 attctcaaca aacttcggcg ggtccatcac gataacgtca aatttttcac cgcgatcgcg
    23281 ataggtacgc agcaatttaa agacatcatc acggacaaac tcagccttgc tcagatccag
    23341 tttgttcagc tcaacgttct gccgtgcaat atccagtgct tcctgggagg tatcaacgct
    23401 gacaacctgg ctgcaaccgc ccatcagtgc cgatacggcg aaaccaccgg tataggagaa
    23461 acagttcagt acgcgtttat tttcaacgta gcggcgggta gccaggcggc tgtctcgctg
    23521 gtccaggtag tagcccgttt tgtgtccgtg ctgtatgtcc accagcagct tcatgccatg
    23581 ttcttcaatc ggcagcaggg caggcggcaa ctcaccggtg acgaggccct gggtcagctc
    23641 catcccttct tttttacgta ccgcaacatc gctgcgatcg taaatcgcac attccgggta
    23701 cagcgtttgc agggcactaa ttaatgccgc gcgctgatat tctgcgccag cactcagcag
    23761 ttgcagcacc agaaaattac cgaaacgatc gatagtaata cccggcaggc catcagattc
    23821 tccggcgatt aaacgatagc tgtcgaggcc atctttttgc gccagccagt cacgccattt
    23881 ttgtgcttgt tgcaaacggc gggaaaaaaa agcaatgtcg atagactcag acgggtcaaa
    23941 cgtccagacg cgcgcccgga tttgcgaagc tggcgaataa gcgccgcgtg ctaaccattt
    24001 tccctgatga tcaacaatat cgatggtttc accgaggctg gctttacctt ccatacgggc
    24061 aacggccccg gaaaagaccc acggatggcg acgaagtaat gatttttcgc gccctttggc
    24121 taacactaaa cgtacactca taatttactt ttctgtcgat gcaaagaaat gggcgtcatt
    24181 gtccggagtt ttccatggat ttgcaacgcg tccattaagg ataaggagag cagcaatgtc
    24241 gaaagtctgc ataattgcct gggtttacgg gcgagttcag ggcgtaggat ttcgctacac
    24301 cacacagtac gaagcgaaaa gactgggctt aaccgggtac gccaaaaatc ttgacgatgg
    24361 cagcgtggaa gtggttgcct gcggtgaaga agggcaggta gaaaaattaa tgcagtggtt
    24421 aaagagtggc ggcccgcgtt ctgcgcgtgt agaacgggtg ctcagcgagc cgcatcatcc
    24481 ctcgggggaa ttaaccgatt ttcgtatccg ttaaatacat tttaccggtt ttggcaggcc
    24541 agcaattttg gtggcttgct tagccggacc tttcggaaac agtcggtata aatagcggct
    24601 gttacctttt tcttcgccaa atttattcgc catcgctttt accagcatac gaatcgccgg
    24661 agaagtattg aattccagat agaaatcacg cacaaaacgc accacttccc agtgttctgg
    24721 cgacagcgaa atcccttcgt tctctgcaat caccactgcc agcggctcac tccactggct
    24781 gctttctttg agatagcctt cggtatccgt ttctatctct ttaccttcga agatcagcat
    24841 aattattact accttaatca gactgccggc agtgtaacaa aaaacaaagc cccgcataaa
    24901 gcgaggctat gaaagtgtta gcgggtgaga ttaatcgcgg ctggcgaagc ccagaatgct
    24961 cagcaggctg acgaagatgt tgtacagcga aacatacagg ctaaccgtgg cacgaatata
    25021 gttcgtttca ccgccatgaa tgatgttgct ggtttcaaac aaaatagcgc cagaggagat
    25081 caaaatgaag accgcgctga tcgccagatg cagagcaggc agctgcagga agatattagc
    25141 aaccatacca atcagcacca ccacaatacc cgccatcagc ataccgccga ggaacgacat
    25201 atctttgcgg gtggtcagca catatgcaga gcagcagaag aacactaacg ccgttccgcc
    25261 cagtgccata gcgattacgt cacccattcc ggcagacaga taggtattca gaattggtcc
    25321 gaggatataa cccagaaaac cggtaaaggc gaatgcggag ataatcccgg tcggcttatt
    25381 cgccatttta taggtcagga acatcaaacc atacataccc accagcgtca gaatcagacc
    25441 cggagatggc agcatcagca ccgtactggc agttgcggta atcgccgaaa aggccagcgt
    25501 caggctcagc agaaaatagg tattacgcag cactttatgg gtgctaagca gtgatgtacg
    25561 gtcatgtgaa gaactaacaa tacgatccat tgagtcactc tctatgacag atgtaattaa
    25621 ttaagcagca taatgataat gcgtaagggc acccagtagt tttacccatc tttacgcatt
    25681 tgatctggaa caggtttaac agcggattat caggtcatta agcaaatata acgccctgag
    25741 aatttcggca ggcaaaagaa aaaggggtta gcatttagct aaccccttat cttatctggc
    25801 ggaagcgcag agattcgaac tctggaaccc tttcgggtcg ccggttttca agaccgaaga
    25861 aaaacaaata aaaatcagtg taatacgtga aattgctgaa aagttaatca aatttaacac
    25921 caagaaaaat caaggtatta ggaagttgct catagctagt ataccaagtc tttttgccag
    25981 aaatttttct ggattgtcta tttgctaacc acgagcggca aatccagtgt tggtgtgatt
    26041 tttgttttac gatcgtaaat caacacctga ttttctgttt tatgcccgct gaaaatttgt
    26101 ttgtcgcgac tgctgccttc gtaatctgag atccccttgg cttttatgtc gtgaaaattg
    26161 cacccgaacg gaacgccaac tttttgctcg gctgcgcgtt tagcctgatt ccaccagtta
    26221 ttcagcgttt tagctatgac tttcccgcct ttggttgtat tgatcacata ttcgcatgta
    26281 caggaagata catttcgggc taactggatc gctgtacgta atcgcggtga ccattccttg
    26341 atttgtttgg tgccggtttt gttttgctca atgtagatcc ctttatccat aatatcctgc
    26401 catttcaact caagcacatc accgagtctt gccgcacaga gataggaaat ctccattgca
    26461 atgcgtaact gtggaattgc ttccgcatat atcgccgcat actgttcatc ggtgatgtaa
    26521 acagtgcggg ctttaagaga gaattttctg actcctttgc atgggttatt cttcacatac
    26581 ccacgctcat atccccaccc gtatactcga ctcaggcttg ccagttcatg atttgcctgg
    26641 gtcttgctct caagccctcg tttatccatg aaaattctta cctgctcagt tttgacatta
    26701 tcagcaagca cttttccgaa taccatcagc aacgccttct gatgttgtcg ataatctttt
    26761 tgggttcggg gggccagttc tgtaaatgca ggggagtcca taaacatgtg ccacaattta
    26821 gctacggtca ttatgttgtg gagttttgct ttttccagtt cataattttg ccagacttta
    26881 gctacgctgg ttttccgcac tcttcctagc cctatacttc ttgtacttcc ttcgggtttc
    26941 cacacgtaac tgtaaccatt cgatctaacc cgcggtggca gtacattatc ttttttattt
    27001 tttcttggtc ttcccattgt tcagcgcctc aaaatcgggt tcagcagaaa ccagttcaga
    27061 tgtttttggc atcgttgtca gaccgtgtgg aatatccctg cggagaacta tgggttcgtt
    27121 tttagggccg attacaaatg ggatgccgtg cagtcttaac tggtgttgct gttttgtgta
    27181 tcgctcgtat ttcgtgatct ctttaatctc tgctggagat agagttaatt cgtacatgtg
    27241 gtcacgtgcc tttacagcat gaccgccgcc actatagctg gtgggcggcg atatgggttg
    27301 aacatcaaaa atcagcctga ctcgggagca gtttttgcca gatggctgaa acgtatttcg
    27361 cctggtaacg
//
LOCUS       DAZFAB010000056        27163 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZFAB010000056 DAZFAB010000000
VERSION     DAZFAB010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27163
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.056"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(173..1837)
                     /gene="glnS"
                     /locus_tag="ACHJWA_002719"
     CDS             complement(173..1837)
                     /gene="glnS"
                     /locus_tag="ACHJWA_002719"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFO4309288.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRTGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRSD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(2040..3986)
                     /gene="nagE"
                     /locus_tag="ACHJWA_002720"
     CDS             complement(2040..3986)
                     /gene="nagE"
                     /locus_tag="ACHJWA_002720"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFO4309289.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            4319..5119
                     /gene="nagB"
                     /locus_tag="ACHJWA_002721"
     CDS             4319..5119
                     /gene="nagB"
                     /locus_tag="ACHJWA_002721"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFO4309290.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5179..6327
                     /gene="nagA"
                     /locus_tag="ACHJWA_002722"
     CDS             5179..6327
                     /gene="nagA"
                     /locus_tag="ACHJWA_002722"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO4309291.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6336..7556
                     /gene="nagC"
                     /locus_tag="ACHJWA_002723"
                     /gene_synonym="nagR"
     CDS             6336..7556
                     /gene="nagC"
                     /locus_tag="ACHJWA_002723"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFO4309292.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7604..8356
                     /gene="nagD"
                     /locus_tag="ACHJWA_002724"
     CDS             7604..8356
                     /gene="nagD"
                     /locus_tag="ACHJWA_002724"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFO4309293.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            8613..10277
                     /gene="asnB"
                     /locus_tag="ACHJWA_002725"
     CDS             8613..10277
                     /gene="asnB"
                     /locus_tag="ACHJWA_002725"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFO4309294.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDKA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10657..10733
                     /locus_tag="ACHJWA_002726"
     tRNA            10657..10733
                     /locus_tag="ACHJWA_002726"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10691..10693,aa:Met,seq:cat)
     gene            10744..10828
                     /locus_tag="ACHJWA_002727"
     tRNA            10744..10828
                     /locus_tag="ACHJWA_002727"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10778..10780,aa:Leu,seq:tag)
     gene            10852..10926
                     /locus_tag="ACHJWA_002728"
     tRNA            10852..10926
                     /locus_tag="ACHJWA_002728"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10884..10886,aa:Gln,seq:ttg)
     gene            10961..11035
                     /locus_tag="ACHJWA_002729"
     tRNA            10961..11035
                     /locus_tag="ACHJWA_002729"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10993..10995,aa:Gln,seq:ttg)
     gene            11051..11127
                     /locus_tag="ACHJWA_002730"
     tRNA            11051..11127
                     /locus_tag="ACHJWA_002730"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11085..11087,aa:Met,seq:cat)
     gene            11176..11250
                     /locus_tag="ACHJWA_002731"
     tRNA            11176..11250
                     /locus_tag="ACHJWA_002731"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11208..11210,aa:Gln,seq:ctg)
     gene            11288..11362
                     /locus_tag="ACHJWA_002732"
     tRNA            11288..11362
                     /locus_tag="ACHJWA_002732"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11320..11322,aa:Gln,seq:ctg)
     gene            complement(11516..12691)
                     /gene="ubiF"
                     /locus_tag="ACHJWA_002733"
     CDS             complement(11516..12691)
                     /gene="ubiF"
                     /locus_tag="ACHJWA_002733"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFO4309295.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFTVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGEVIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQVEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            12837..14261
                     /gene="miaB"
                     /locus_tag="ACHJWA_002734"
     CDS             12837..14261
                     /gene="miaB"
                     /locus_tag="ACHJWA_002734"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFO4309296.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            14414..15454
                     /locus_tag="ACHJWA_002735"
     CDS             14414..15454
                     /locus_tag="ACHJWA_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFO4309297.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            15451..15918
                     /gene="ybeY"
                     /locus_tag="ACHJWA_002736"
     CDS             15451..15918
                     /gene="ybeY"
                     /locus_tag="ACHJWA_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFO4309298.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQMVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            16008..16886
                     /gene="corC"
                     /locus_tag="ACHJWA_002737"
     CDS             16008..16886
                     /gene="corC"
                     /locus_tag="ACHJWA_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFO4309299.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            16911..18449
                     /gene="lnt"
                     /locus_tag="ACHJWA_002738"
     CDS             16911..18449
                     /gene="lnt"
                     /locus_tag="ACHJWA_002738"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFO4309300.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIRWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            18846..19754
                     /gene="gltI"
                     /locus_tag="ACHJWA_002739"
     CDS             18846..19754
                     /gene="gltI"
                     /locus_tag="ACHJWA_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFO4309301.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            19924..20664
                     /gene="gltJ"
                     /locus_tag="ACHJWA_002740"
     CDS             19924..20664
                     /gene="gltJ"
                     /locus_tag="ACHJWA_002740"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFO4309302.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQASKLLDYSAHAWESFTAITLA
                     YVFINAFIMLVMTLVERKVRLPGNMGGK"
     gene            20664..21338
                     /gene="gltK"
                     /locus_tag="ACHJWA_002741"
     CDS             20664..21338
                     /gene="gltK"
                     /locus_tag="ACHJWA_002741"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFO4309303.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            21338..22063
                     /gene="gltL"
                     /locus_tag="ACHJWA_002742"
     CDS             21338..22063
                     /gene="gltL"
                     /locus_tag="ACHJWA_002742"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFO4309304.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            22181..23116
                     /gene="rihA"
                     /locus_tag="ACHJWA_002743"
     CDS             22181..23116
                     /gene="rihA"
                     /locus_tag="ACHJWA_002743"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFO4309305.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            23200..24870
                     /gene="hscC"
                     /locus_tag="ACHJWA_002744"
     CDS             23200..24870
                     /gene="hscC"
                     /locus_tag="ACHJWA_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HFO4309306.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDEVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPIHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKRTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRQASVYLAIEIP"
     gene            complement(24930..26381)
                     /gene="djlC"
                     /locus_tag="ACHJWA_002745"
                     /pseudo
     CDS             complement(24930..26381)
                     /gene="djlC"
                     /locus_tag="ACHJWA_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
     gene            complement(26378..27085)
                     /locus_tag="ACHJWA_002746"
     CDS             complement(26378..27085)
                     /locus_tag="ACHJWA_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO4309307.1"
                     /translation="MNKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNKWLTEDESLWLQSRVYERAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
BASE COUNT         6670 a         7048 c         6893 g         6552 t
ORIGIN      
        1 atgcggcgtg aacgccttat ccggcctaca aaagcacgca aactcaatat attgcagaga
       61 tcatgtaggc ctgataagcg tagcgcatca ggcaatttag cgtttgaaac tgtttgtctg
      121 ttccttgccg gatgcgacgt aaacgcccca tccggcatag cgaaacttaa aattactcgc
      181 ctactttcgc ccaggtatca cgcagcccaa cggtgcggtt aaataccggt ttttccgccg
      241 tagaatggcg gctatcgagg cagaagtaac cttcacgctc aaactggaat gctttacccg
      301 caaccgcatc tttcagcgac ggttcagcaa agccctgttt gatcaccagc gattccgggt
      361 taatcaccga caggaaatca tccgcagcac ctgggttcgg cacgctgaac agacggtcat
      421 acaaacggat ttcaaccggc agcgcatgtg ccgcgctcac ccagtgaata acacctttga
      481 ctttacgacc atctgccgga tctttgctta aggtatcggc gtcataagta cagaagatgg
      541 tggtgatatt accttcggca tctttctcga cgcgttctgc cttaatcaca taagcattac
      601 gcagacgcac ttctttaccc agcaccagac gtttgtactg cttgttagct tcttcgcgga
      661 aatcggagcg atcaatccaa atctcaccgc taaacggcac ctgacggctc cccatttccg
      721 gtttgttcgg atggttcggc atggtaacca tttcgccttc gccctgatag ttttcgataa
      781 ccagtttcac cggatcgata accgccattg cgcgcggcgc attttcgttg agatcttcac
      841 ggatgcagga ttccagcgac gccatctcaa tggtgttgtc ctgcttggtc acgccgatgc
      901 gtttgcagaa ctcacgaata gaagccgcag tgtaaccacg acgacgcaga ccggaaatgg
      961 tcggcatacg cgggtcatcc cagccttcaa cgtgcttgtc ggtcaccagc aagttcagct
     1021 tacgcttgga catcacagtg tattccagat tcaggcgcga gaattcgtac tggcgcgggt
     1081 gaacaggaat ggtgatgttg tccagtaccc agtcgtacag acgacggtta tcctggaact
     1141 caagcgtaca cagagagtgc gtaatacctt ccagggcatc gctgatgcag tgggtgaagt
     1201 cgtacatcgg gtagatgcac cacttgttgc cagtctggtg gtgttcagca aatttaatgc
     1261 ggtacagcac cggatcgcgc atcacgataa acggtgaagc catgtcgatt ttcgcacgca
     1321 ggcaggcttt accttcttca aaaccgccgg tacgcatttt ctcgaacagc gccaggttct
     1381 cttcaacgct acggtcgcgg tacgggctgt ttttaccagg ctgcgtcagg gtgccgcggt
     1441 attcgcggat ctgttccggc gtcagttcat caacgtacgc caggccttta ttgatcagtt
     1501 cgatcgcata ggcgtggagc tgatcaaaat aatcggagga gtaacggacg ttaccagacc
     1561 agtgaaaacc taaccactca acgtcgtttt tgatcgaatc gacatactcg atatcttctt
     1621 ttaccgggtt agtgtcgtcg aaacgcaggt tgcactggcc tttatagtcc tgggcgatcc
     1681 cgaagttcag gcagatagat ttcgcatggc caatatgcag atagccattc ggctccggcg
     1741 ggaaacgggt gtgtactgtg gtgtgcttac cactggccag atcttcatcg atgatctgac
     1801 ggataaagtt agtcgggcgg gcttctgcct cactcatcgt ggattcctca aagcgtaaac
     1861 aacgtataac ggcgtatgat cttataagcg ggacaggctg acaactgtta gttagtgaaa
     1921 atcgaaaatc tcactgagat tatggggatg attgatgaaa aaaaaagcgg cagagaagat
     1981 ctccaccgct tgtcattgtt ggatgcgacg ctcaagcgtc gcatcaggca taaagcagat
     2041 tactttttga tttcatacag cggtgtttga cccgccacaa catggccctg agctttaatg
     2101 atcaagccac tgaaatcgtc gatattgctg caaaccaccg ggctaatcat cgaacgggcg
     2161 ttagcgttca ggtaatccag atccatttcc agaatcggtt gccctgcgct tacctgcgcg
     2221 ccctcttcca ccagacgttt aaagccttta ccttccagcg ctacggtatc gatacccatg
     2281 tgaacgacaa tctccgcgcc tttttcggtt tccaggcaga acgcgtggtt ggtgttgaag
     2341 attttcacga ttgttcctgc ggctggtgat acgacgattt tatctgtcgg tttcaccgcc
     2401 acaccgtcac ccaccgcttt gctggcgaat gcttcgtcag gaacctgatc cagtgccacg
     2461 acatcaccgg taatcggcga taccagctcc gcgatagata ccgcgtttgg tacagcctgc
     2521 ggttttgcta caggagcggc agttgccgga gttgcttcag cagaggcagc tgctaccggg
     2581 ccacgggcaa cgactttctt catcgcatcg ccgatggatt ctgctttcgc gccaacaatc
     2641 acctgaatag tctgtttgtt cagttttact accccagaag cccccagacg tttacacatc
     2701 gtatcgttaa cgcgggcaga gtcagccacg gtaaggcgca gacgggtgat acaggcgtca
     2761 attgctttca ggttgtcagt gccgccaacc gcagcaatat agttggtcgc cagttgattg
     2821 aggccttctt cagtgttgct gttggcttct tcagtaacga tctcgtcttc tttatcttca
     2881 cgacccggcg ttttcaggtt gaacatgcgg ataaccaaac tgaacaccac gaagtagata
     2941 gcgaagaaga caacacccat aaccagcagc atccagacgt tctggctggc ggccggcagg
     3001 ttatacatca acgcgtagtc gatagccccc gcagagaaag agaagccggc atggataccc
     3061 agcagcgttg ccacaaacag gctgataccg gtcagcagtg cgtgcaggag gtacagcagc
     3121 ggagcaagga acatgaacag gaattccagc ggctcagtca caccggtcag gaacgcagta
     3181 acagcaacag aaagcagcat cccgccaacc atcggacgac gctctttcgg tgctgcgaag
     3241 tacatcgcca gcgccgcacc cggcaggccg aacatcatga tcgggaagaa gccggacatg
     3301 aacatccccg cggtgccgtc accagcgtag aaacggttga tgtcgccgtg gaaaaccgta
     3361 cccgccgcgt tggtgaattc accaatctgg aaccaggcga tggtgttcag cacctgatgc
     3421 agaccggttg ggatcaacag acggttgatg aaaccaaaga taccggaacc cagcgcgccc
     3481 gcagaaacga tccactcgcc gcctgcatgg atagcgtgct gtaccggcgg ccagacgtaa
     3541 ccaaaaatgg ccgccagcac cagacagaag aagccggtgg cgatcggcac aaagcgtttg
     3601 ccgccgaaga agctcaggaa gtccggcagt ttaatatcgg accaacggtt ataggctgcg
     3661 ccaccaacca gaccggtaat gatacccgcc agtacaccca tgttaatttc tgggttgatg
     3721 gtcaccatcg ctttggttaa cacaaagtaa cctaccgccc ccgccagcgc cgccgcacct
     3781 gcgctgtctt tcgaccagct ggatgccaca ccgatggcga agattaatgc gaggttatca
     3841 aaaatcgcac cgcccgcctg ggcaataaac gcaacgttaa gtaaatctgg ctgaccgaat
     3901 cgcagcaaca gtgccgccac cggcagcacc gcgataggga gctgtaacgc cctaccgagt
     3961 cgctggaaaa aacctaaaat attcatctta ttccccctac gagaacccta tttggctcgt
     4021 ttaaagccgt atttttattg tgctgcaaat tgtactgccg atgttctgta atcagattgt
     4081 tagatcatct gccacagagt gtgtgaaaat ttaattcgta tcgcaaatta aacgagtgtc
     4141 ttttgtgagt tttgtcacca aatatcgtta tcatcactcc cttttactgg ctaaaccaga
     4201 aaacttattt tatcattcaa aaaatcaggt cggattgacg cctgtctgcg caaatccagg
     4261 ttacgcttaa agatgcctaa tccgccaacg gcttacattt tacttattga ggtgaataat
     4321 gagactgatc cccctgacta ccgctgaaca ggtcggcaaa tgggctgctc gccatatcgt
     4381 caatcgtatc aatgcgttca aaccgactgc tgatcgtccg tttgttctgg gcctgccaac
     4441 tggcggcacg ccaatgacca cctataaagc gttagtcgaa atgcataaag caggccaggt
     4501 cagctttaag cacgttgtca ccttcaacat ggacgaatat gtcggtctgc cgaaagagca
     4561 tccggaaagc tactacagct ttatgcaccg taatttcttc gatcacgttg atattccagc
     4621 agaaaacatc aaccttctca acggcaacgc cccggatatc gacgccgagt gccgccagta
     4681 tgaagaaaaa atccgttctt acggaaaaat tcatctgttt atgggcggtg taggtaacga
     4741 cggtcatatt gcatttaacg aaccggcgtc ttctctggct tctcgtactc gtatcaaaac
     4801 cctgactcat gacactcgcg tcgcgaactc tcgtttcttt gataacgatg tcaatcaggt
     4861 gccaaaatat gccctgactg tcggtgttgg tacactgctg gatgccgaag aagtgatgat
     4921 tctggtgctg ggtagccaga aagcactggc gctgcaggcc gcagttgaag gttgcgtgaa
     4981 ccatatgtgg accatcagct gtctgcaact gcatccgaaa gccatcatgg tgtgcgatga
     5041 gccttccacc atggagctga aagttaagac tttaagatat ttcaatgaat tagaagcaga
     5101 aaatatcaaa ggtctgtaat tgttatccct gccctggctc cttgctcagg gcaatatttt
     5161 ttaaaatcgg gggtcagaat gtatgcatta acccagggcc ggatctttac cggccacgaa
     5221 tttcttgatg accacgcggt tgttatcgct gatggcctga ttaaaagcgt ctgtccggta
     5281 gcggaactgc cgccagagat cgaacaacgt tcactgaacg gggccattct ctcccccggt
     5341 tttatcgatg tgcagttaaa cggctgcggc ggcgtacagt ttaacgacac cgctgaagcg
     5401 gtcagcgtgg aaacgctgga aatcatgcag aaagccaatg agaaatcagg ctgtactaac
     5461 tatctgccga cgcttatcac caccagcgat gagctgatga aacagggcgt gcgcgttatg
     5521 cgcgagtacc tggcaaaaca tccgaatcag gcgttaggtc tgcatctgga aggcccgtgg
     5581 ctgaatctgg tgaaaaaagg tacccataat ccgaattttg tgcgtaagcc tgatgccgcg
     5641 ctggtcgatt tcctgtgtga aaacgccgac gtcattacca aagtgaccct ggcaccggaa
     5701 atggttcctg cggaagtcat cagcaaactg gcaaatgccg ggattgtggt ttctgccggt
     5761 cactccaacg cgacgttgaa agaagcgaaa gccggtttcc gcgcggggat tacctttgcc
     5821 acccatctgt acaacgcgat gccgtatatt accggtcgtg aaccgggcct ggcgggcgcg
     5881 atcctcgatg aagctgacat ttattgcggt attatcgctg atggcctgca tgttgattac
     5941 gccaacattc gtaatgctaa acgccttaaa ggcgacaaac tgtgtctggt taccgacgcc
     6001 accgcgccag caggtgccaa cattgaacag ttcatttttg cgggtaaaac aatatactac
     6061 cgtaacggac tttgtgtgga tgagaacggt acgttaagcg gttcatcctt aaccatgatt
     6121 gaaggcgtgc gtaatctggt cgaacattgc ggtatcgcac tggatgaagt gctgcgtatg
     6181 gcgacgctct atccggcgcg tgcgattggc gttgagaaac gtctcggcac actcgccgca
     6241 ggtaaagtag ccaacctgac cgcattcaca cctgatttta aaatcaccaa gaccatcgtt
     6301 aacggtaacg aggtcgtaac tcaataagag aaagtatgac accaggcgga caagctcaga
     6361 taggtaatgt tgatctcgta aaacagctta acagcgcggc ggtttatcgc ctgattgacc
     6421 agtacgggcc aatctcgcgg attcagattg ccgagcaaag ccagcttgcc cccgccagcg
     6481 taaccaaaat tacgcgtcag cttatcgaac gcgggctgat caaagaagtt gatcagcagg
     6541 cctccaccgg gggccgccgc gctatctcta tcgtcaccga aacccgcaat ttccacgcaa
     6601 tcggcgtacg gcttggtcgt cacgacgcca ccatcaccct gtttgatctc agcagcaaag
     6661 tgctggcaga agaacattac ccgctgccgg aacgtaccca gcagacgctg gaacatgccc
     6721 tgctgaatgc cattgctcag tttattgata gctaccagcg caaactgcgc gagctgatcg
     6781 ccatttccgt tatcctgcca ggacttgttg acccggacag cggcaaaatt cattacatgc
     6841 cgcatattca ggtagaaaac tgggggctgg tagaagcact ggaagagcgt tttaaagtga
     6901 cctgtttcgt tggtcacgat atccgtagtc tggcgctggc ggagcactac ttcggtgcaa
     6961 gtcaggattg cgaagactcc attctggtgc gtgtccatcg cggaaccggg gccgggatta
     7021 tctctaacgg gcgcattttt attggccgca acggcaacgt cggtgaaatt ggccatattc
     7081 aggtcgaacc gctgggtgaa cgctgccact gcggcaactt tggctgcctg gaaactatcg
     7141 ctgccaacgc cgctattgaa caacgggtgt tgaatctgtt aaagcagggc taccagagcc
     7201 gcgtgccgct ggacgactgc accatcaaaa ctatctgcaa agccgcgaac aaaggcgata
     7261 gcctggcctc ggaagtgatt gagtatgtcg gtcgtcatct gggcaaaacc atcgccattg
     7321 ctatcaactt atttaatccg caaaaaattg ttattgccgg tgaaatcacc gaagccgata
     7381 aagtgctgct ccctgctatt gaaagctgca ttaatactca ggcgctgaag gcgtttcgta
     7441 ctaatctgcc ggtggtacgt tctgagctag accaccgctc ggcaatcggt gcttttgcgc
     7501 tggtaaaacg cgccatgctc aacggtattt tgctccagca tttgctggaa aattaatgtg
     7561 cttttatagt ggcgcttatt gttgtcaata ttctgggtag tccatgacca ttaaaaatgt
     7621 aatttgcgat atcgacggcg tgctgatgca cgataacgtc gccgtaccgg gtgcagcgga
     7681 atttttgcac gggattatgg ataaaggcct gccgctggtg ttgctgacca actatccttc
     7741 gcagaccggg caagatctgg cgaaccgctt tgccactgcc ggtgtcgatg tacctgacag
     7801 cgtgttttat acctctgcga tggcgaccgc cgatttcctg cgtcgccagg aaggcaagaa
     7861 agcgtatgtt gtgggcgaag gcgcactgat tcatgaactg tacaaagccg gtttcactat
     7921 taccgatgtg aaccctgatt ttgtgattgt tggcgaaacg cgttcctaca actgggacat
     7981 gatgcataaa gcggcctatt tcgtcgctaa cggtgcacgc tttatcgcca ccaacccgga
     8041 cacccacggg cgcggttttt atccggcctg tggcgcgttg tgtgcaggga ttgagaaaat
     8101 ctccgggcgc aaaccgttct atgttggtaa gccgagtccg tggattatcc gcgcggcatt
     8161 aaacaaaatg caggcacatt cggaagaaac ggtgattgtc ggcgataacc tgcgtaccga
     8221 tattctggcc ggcttccagg caggtctgga gacgattctg gtgctttctg gtgtttcgtc
     8281 gctcgacgat atcgacagta tgcctttccg ccccagctgg atttacccgt cagtcgctga
     8341 aatcgacgtt atctgaatag caaccacgtc tcaggacgtg gtttcttatt tttacccatc
     8401 attaaaatca tcccattcaa taaaacccgc caaaaattat cgatccatca ataaatacat
     8461 caccgcaccc tccccttttt cacaaatcag caatcaccat tacgtttttt attttttcac
     8521 cgccaaatcg cttgcgcaca gtgctgcttt gcggcatttt tttaaacaag caaacacaac
     8581 aagcaacaaa taccaggtta acggagaagg ttatgtgttc aatttttggc gtattcgata
     8641 tcaaaacaga cgcagttgag ctgcgtaaga aagcactcga gctgtcacgc ctgatgcgtc
     8701 atcgtggccc ggactggtcc ggtatttatg ccagcgataa agccattctc gctcacgaac
     8761 gtctgtcaat tgttgacgtt aacgcggggg cgcaacctct ctacaaccaa caaaaaaccc
     8821 acgtactggc ggtaaacggt gaaatctaca atcaccaggc attgcgcgct gaatatggcg
     8881 atcgttatca gttccagaca gggtctgact gtgaagtgat cctcgcgctg tatcaggaaa
     8941 aagggccgga atttctcgac gacttgcagg gcatgtttgc ctttgccctg tacgacagcg
     9001 aaaaagatgc ctacctgatt ggtcgcgacc atctggggat catcccactg tatatgggct
     9061 atgacgaaca cggtcagctg tatgtggcct cagaaatgaa agccctggtg ccagtttgcc
     9121 gcacgattaa agagttcccg gcggggagct atttgtggag tcaggacggc gaaattcgtt
     9181 cttattatca tcgcgactgg ttcgactttg atgcagtgaa agataacgtg accgacaaaa
     9241 acgagctgcg tcaggctctg gaagattccg ttaaaagcca tctgatgtct gatgtgcctt
     9301 acggtgtgct gctttctggt ggtctggatt cctcaattat ttccgctatc accaagaaat
     9361 acgcagcccg tcgcgtggaa gatcaggaac gctctgaagc ctggtggccg cagttacact
     9421 cctttgctgt aggtctgccg ggttcaccgg atctgaaagc agcccaggaa gtggcaaacc
     9481 atctgggcac tgtgcatcat gaaattcact tcactgtaca ggaaggtctg gacgccatcc
     9541 gcgacgtgat ttaccacatc gaaacctatg atgtgaccac tattcgcgct tcaacaccga
     9601 tgtatttaat gtcgcgtaag atcaaagcga tgggcattaa aatggtgctg tccggtgaag
     9661 gttctgatga agtgttcggc ggttatcttt acttccacaa agcaccgaat gccaaagaac
     9721 tgcatgaaga gacggtgcgt aaactgctgg ccctgcatat gtatgactgc gcgcgtgcca
     9781 acaaagcgat gtcagcctgg ggcgtggaag cacgcgttcc gttcctcgac aagaaattcc
     9841 tcgacgtggc gatgcgcatt aacccgcagg ataaaatgtg cggtaacggc aagatggaaa
     9901 aacacatcct gcgtgaatgt tttgagtcat acctgcccgc aagcgtggcc tggcggcaga
     9961 aagagcagtt ctccgatggc gtcggttaca gttggatcga caccctgaaa gaagtggcgg
    10021 cgcagcaggt ttctgatcaa cagctggaaa ctgcccgctt ccgcttcccg tacaacacac
    10081 cgacctcaaa agaagcgtat ctgtaccggg agatctttga agaactattc ccgcttccga
    10141 gcgccgctga gtgcgtgccg ggcggtcctt ccgtcgcgtg ttcttccgct aaagcgatcg
    10201 aatgggatga agcgttcaag aaaatggacg atccgtctgg tcgcgcggtt ggtgttcacc
    10261 agtcggcgta taagtaagaa atgcgaaaca gccccggtga aatccggggc tgtttcagtt
    10321 attgaggtgc ttgttgattc tgatatgcag taaggaaatg ttccgggttt gccacatcac
    10381 tgtttgacaa ctcatcactg taggccagta tttcatagat taatttccca ccactattgt
    10441 taaattccga atttcatcct gtatgacaaa agaatggctg tagggtggga aaaaacacaa
    10501 agtaaacaat aattgacgaa tatagcgcca cgctgttcgc aacctaacca aacagtcact
    10561 ttcgagcaat tttccttgaa aaagaggttg acgctgcaag gctctatacg cataatgcgc
    10621 cccgcaacgc cgataaggta tcgcgaaaaa aaagatggct acgtagctca gttggttaga
    10681 gcacatcact cataatgatg gggtcacagg ttcgaatccc gtcgtagcca ccatcttttt
    10741 tttgcgggag tggcgaaatt ggtagacgca ccagatttag gttctggcgc cgcaaggtgt
    10801 gcgagttcaa gtctcgcctc ccgcaccatt caccagaaag cgttgtacgg atggggtatc
    10861 gccaagcggt aaggcaccgg tttttgatac cggcattccc tggttcgaat ccaggtaccc
    10921 cagccatctt ctttgagtaa gcggttcacc gcccggttat tggggtatcg ccaagcggta
    10981 aggcaccggt ttttgatacc ggcattccct ggttcgaatc caggtacccc agccatcgaa
    11041 gaaacaatct ggctacgtag ctcagttggt tagagcacat cactcataat gatggggtca
    11101 caggttcgaa tcccgtcgta gccaccaaat tctgaatgtg tcgaatacgt tcggcaaatt
    11161 caaaaaccaa tttgttgggg tatcgccaag cggtaaggca ccggattctg attccggcat
    11221 tccgaggttc gaatcctcgt accccagcca atttattcaa gacgcttacc ttgtaagtgc
    11281 acccagttgg ggtatcgcca agcggtaagg caccggattc tgattccggc attccgaggt
    11341 tcgaatcctc gtaccccagc cacattaaaa aagctcgctt cggcgagctt tttgcttttc
    11401 tgcgttcact caatgtcgaa tgcgatgttg acacgtctta tccttcaatg tcggatgcga
    11461 cgctgccgcg tcttatccga cctacggttg gcacgcatcc ggcaatgttg taaggctaca
    11521 accctaacgc atatttcagc gcctgacgtt tcaacacgcc agcacgctcc gccgccatca
    11581 aaccaagatt acgcacaaaa cgcagcggcg gcagattatt actgaatccg gcataaaaca
    11641 gatccatacc gctctgcata atgaagttat ccgccatgcg ccgcatctgg taacgcttga
    11701 ggacaggata actggcccac gcttcgccgt agctgcgggc attcaccaga acatcaatca
    11761 gggcatcgac atcacgataa ccaagattca ccccctgccc cgccagcgga tggatggtgt
    11821 gcgcggcatc acccaccagc gcaagccccg gctgcacata ctgcaacgca tggcggcgtg
    11881 tcagcggaaa cgcaccagcg gcaagcggtg taacgtaacc cagacgcgac gggaaatgct
    11941 tcgcgatttc cacctgtaac tgcgccatat tcatattctg caactggcga atgcgcgccg
    12001 gagagtcgta ccacaccagc gatgcccagt tatcaaacag cggcagaaac gcgcgcggtc
    12061 catccggggt aaattgctgc caggtgctat cgccgggatc gttctcgcac tggacgctaa
    12121 tcaacatgca cgactgcgca tactgccatg catgaacgcc aattcccgcc atctgccgca
    12181 cctgcgaatt tgcgccgtcg gcaccaatca ccagcttcgc gcgaatcact tcaccgcctt
    12241 tcagctccag ctcctgaaga tcattatctc gatgtaatgc aatcagcgag gttggcacac
    12301 gtaacgttac tttcggatgc gcttccagcg cctgccacaa tgcctgttgc aggacagtgt
    12361 tttctaccat atagccaagc aacggcaact ttaattcggc tgcgtcaaat accacatgcg
    12421 ccgtttccca ctcccacgtt tccagtctgc ggtaaggatg gcaacgcata gcctgtactg
    12481 catcccagac ccctaaccct ttaagcaatg ataccgaagc cgcgctgatc gccgagatcc
    12541 gcacgtccgg ttggctatca gcgacaaacg gtgcaggttc tgcgtgctcg atcaccgtta
    12601 ccgtaaatcc gtgctgtgcc agccccagcg ccagtgcgcc gccgaccatt cctccgccga
    12661 caatggcaat ttccgttggt tgatttgtca tggtcaatca tcctgttagc aatatgcttt
    12721 aagtttacag gatttttacc cccaagggct gataacgctc atactggtca caacggcagc
    12781 aaagcattac actatgcgcc ctgcattcct ggctactatt tcgcaagagc aagtcgatga
    12841 ccaaaaaact ccatattaaa acctggggct gtcagatgaa cgagtacgat tcatcgaaga
    12901 tggccgatct gctggatgcc acccacggct atcaactgac cgacgtggcg gaagaagcgg
    12961 atgtgctgct gctgaacacc tgctcaatcc gcgagaaggc tcaggaaaaa gtcttccatc
    13021 agttgggtcg ctggaaactg ttaaaagaga agaatccaga cctgattatc ggcgtcggtg
    13081 gctgcgtggc atcgcaagaa ggcgagcaca ttcgccagcg cgcccactat gtcgatatta
    13141 tttttgggcc gcaaacgctg caccgtctgc cggagatgat caactccgtg cgcggcgacc
    13201 gcagcccggt tgtagatatc agcttcccgg aaatcgagaa gtttgaccgt ctaccggaac
    13261 cgcgcgccga agggccgacc gcgtttgtct ccatcatgga aggctgcaat aaatattgca
    13321 cctactgcgt ggtgccttac acccgtggtg aagaggtaag ccgtccgtct gacgatattc
    13381 tgtttgagat tgcccagctt gcggctcagg gcgtgcgtga agtcaacctg ctcggtcaga
    13441 acgtgaacgc ctggcgtggt gaaaactacg acggcaccac cggatcgttt gccgatctgt
    13501 tgcgtctggt tgctgcgatc gacgggatcg atcgtattcg ctttaccacc agccatccga
    13561 tcgaattcac cgacgatatc atcgaagtgt atcgcgacac gccggagctg gtgagcttcc
    13621 tgcatctgcc ggtacagagc ggttccgatc gcattctgaa cctgatggga cgtacccata
    13681 cggcgctgga gtacaaagcg atcatccgta aactgcgtgc ggcgcgtcca gatattcaga
    13741 tcagttctga cttcatcgtc ggcttccctg gcgaaaccac cgaagacttc gagaaaacga
    13801 tgaagctgat tgccgacgtc aatttcgaca tgagctacag ctttatcttc tctgcacgtc
    13861 cgggtacacc agccgccgat atggttgatg atgttccgga agaagagaag aagcagcgtc
    13921 tgtatattct gcaagaacgt atcaatcagc aagcgatggc gtggagccgc cggatgctcg
    13981 gcaccaccca gcgtattctg gtagaaggta catcgcgtaa gagcatcatg gagctttccg
    14041 ggcgtacgga aaataaccgc gtggtcaact tcgaaggcac gccggatatg atcggtaaat
    14101 tcgtcgatgt agaaattacc gacgtctacc cgaactctct gcgcggtaaa gtggtgcgta
    14161 ctgaagatga aatgggtctg cgcgtggcag aaacaccaga atcggtgatt gcccgtactc
    14221 gcaaagaaaa cgaccttggc gtgggttatt atcagccgta attctcaggc ccgccgttcc
    14281 ggtgggcctt gttttttctc tctttatccc catctttatc gcaatgcttg cgcctttttc
    14341 ctgtggcgtg aataatttcc ttataggccg gtgaatgttc agcttccggc ggcatacaga
    14401 taagaggaac ggtttgaaca tagacactcg cgaaatcacc ctggagccag cagacaacgc
    14461 gcgtctgttg agcctgtgcg gcccgtttga tgacaacatc aagcagctcg aacgccgtct
    14521 cggcatcgag atcaatcgcc gcgataacca ctttaaactg accggccgtc cgatttgcgt
    14581 caccgctgcg gcagacattc tgcgtagcct gtatgtcgat actgccccga tgcgcggtca
    14641 gattcaggat atcgaaccgg aacagatcca ccttgcgatt aaagaagcac gggtactgga
    14701 gcaaagcgcg gagagcgtgc cggagtacgg caaagcggtc aatatcaaaa ccaaacgcgg
    14761 cgtaattaag ccgcgcacgc caaaccaggc gcagtacatc gccaatattc tcgaccatga
    14821 cattaccttc ggcgttggcc cggcgggtac gggtaaaacc tacctggcag tggctgcggc
    14881 agttgatgcc ctggagcgtc aggaaattcg ccgtattctg ctgactcgtc cggcagtaga
    14941 agccggtgag aaactgggct tcctgcctgg cgatttaagc cagaaagtag acccgtatct
    15001 gcgcccgctg tacgacgcgc tgtttgaaat gctgggtttt gagaaagtcg agaaactgat
    15061 tgagcgcaac gttattgaag tcgcaccgct ggcctatatg cgtggtcgta cgctgaacga
    15121 cgcatttatc attctcgatg agagccagaa cactaccatc gaacagatga agatgttcct
    15181 gacccgtatc ggttttaact caaaagcggt tatcaccggc gacgtcacac agatcgacct
    15241 gccgcgtaat actaaatcag gcttacgtca tgccatcgaa gtgctggccg atgtcgaaga
    15301 gatcagcttt aacttcttcc acagcgaaga cgtggttcgt cacccggtgg tggcgcgtat
    15361 cgttaacgcc tatgaagcct gggaagaagc cgaacaaaaa cgtaaagcgg cgctggcggc
    15421 agaacgcaag cgcgaagaac aggaacaaaa atgagtcagg tgatcctcga tttacaactg
    15481 gcatgtgaag ataactccgg attgccggaa gagagccagt ttcaaacatg gctgaatgcg
    15541 gtgatcccgc agtttcagga agagtcggaa gtgacgattc gcgtggtcga taccgccgaa
    15601 agccacagtc tcaatctgac ctatcgcggt aaggataagc cgaccaacgt gctctccttc
    15661 ccgtttgaag tgccgcctgg catggagatg tcgctactgg gcgatctggt tatctgccgt
    15721 cagatggttg agaaggaagc tcaggagcaa ggcaaaccac tggaggcgca ctgggcgcat
    15781 atggtggtgc acggcagtct gcatttgtta ggttacgatc acatcgaaga tgacgaagca
    15841 gaagaaatgg aagccctcga aacagagatt atgcttgctc tgggctatga ggatccgtac
    15901 attgccgaga aagaataatt tgccagccat ttgactggca gacgtccccg ccgttggtga
    15961 ttaacgacgg cgttttgatt aactaacatg agaacccata cgacgccatg agcgacgaca
    16021 attcacacag tagtgacacg ataagcaaca agaagggatt tttctccctg ttactcagcc
    16081 aacttttcca cggtgaaccg aaaaaccgtg acgaactgct ggcgctgatc cgtgattccg
    16141 ggcagaacga ccttatcgac gaagatacgc gcgatatgct cgaaggggtg atggacatcg
    16201 cagaccaacg cgtccgcgac atcatgatcc cccgctccca gatgattacc ctgaaacgca
    16261 accagacgct ggacgaatgc cttgatgtca tcatcgagtc cgcccactca cgtttcccgg
    16321 tgattagcga agacaaagat cacattgaag ggattctgat ggcgaaagac ttgctgccgt
    16381 ttatgcgcag cgatgctgaa gccttcagca tggacaaagt gttacgtcag gcggttgtcg
    16441 ttcctgaaag taagcgcgta gaccggatgc tgaaagagtt tcgctctcag cgttaccaca
    16501 tggcgatcgt tattgacgaa ttcggtgggg tttccggtct ggtaaccatt gaagacatcc
    16561 tggaactgat tgttggtgag attgaagacg aatatgacga agaagatgat atcgacttcc
    16621 gtcagctgag tcgtcatacc tggaccgtgc gcgcactggc ttccattgaa gacttcaacg
    16681 aagcgttcgg cacccacttt agcgatgaag aagtcgacac tatcggtggt ctggtgatgc
    16741 aggcatttgg gcatcttccg gcacgtggcg aaaccatcga catcgacggt taccagttca
    16801 aagtggcgat ggccgacagt cggcgtatta ttcaggttca tgtcaaaatc ccggatgact
    16861 caccccagcc gaagctggat gaataaaacc gaaactggat agataactac atggcttttg
    16921 cctcattaat tgaacgccag cgcattcgcc tgctgctggc gttattattc ggtgcctgcg
    16981 gaacgctggc cttctctcct tacgacgtct ggcctgcggc gattatttcg ctgatggggc
    17041 ttcaggcgtt gacctttaac cgccgtccac tccagtctgc cgctattggc ttttgctggg
    17101 gatttggcct ctttggcagc ggtattaact gggtctatgt cagcatcgcg acctttggcg
    17161 gaatgcctgg cccggttaac atcttcctgg tggtactgct ggcggcgtat ttgtcgctgt
    17221 ataccggact gtttgctggc gtgctgtcgc gcctgtggcc gaaaaccacc tggctgcgcg
    17281 tggcgattgc cgcccctgct ctctggcaag tgaccgagtt tctgcgcggt tgggtactga
    17341 caggcttccc gtggttacag ttcggctata gccagattga tggcccgtta aaagggctgg
    17401 caccgctaat gggcgtggaa gccattaact tcctgcttat gatggttagc ggcctgctgg
    17461 cactggcgtt agtcaaacgc aactggcgtc cgctggtggt ggccgtcgtg ctgtttgccc
    17521 ttcccttccc gctgcgttac atccggtggt ttaccccaca accggagaaa accattcagg
    17581 tttcgatggt tcagggcgat attccgcaat cgctgaaatg ggacgaaggc cagcttctta
    17641 atacgctgaa gatttactac aacgcaacgg caccgctgat gggcaaatca tcgttgatta
    17701 tctggccgga gtcggcgata accgatctgg aaattaatca gcaaccgttc ctcaaagcac
    17761 tggacggtga gttgcgtgat aaaggtagct cgctggtgac cgggattgtt gacgcgcgtc
    17821 ttaataagca gaaccgttac gatacctaca acaccatcat cacactgggt aaaggtgcac
    17881 cgtacagcta cgaatcagcc gatcgctata acaaaaacca tctggtgccg tttggcgagt
    17941 ttgtcccgct ggagtcgatt ctgcgtccgt tagcaccgtt ctttgatctg ccgatgtcgt
    18001 cgttcagccg tgggccatat atccagccgc cgctgtcggc aaatggtatt gagcttactg
    18061 cggctatttg ctacgagatc attctcggcg agcaagtgcg cgataacttc cgcccggata
    18121 ccgactatct gctgactatc tccaacgatg cgtggtttgg taagtctatt ggtccatggc
    18181 agcacttcca gatggcgcga atgcgtgcgc tggagctggc gcgcccactg ttgcgcagca
    18241 ccaacaacgg cattacggcg gtgattggcc cgcagggtga gattcaggcg atgatcccgc
    18301 agttcacccg cgaggtgtta accactaacg tgacgccgac caccggactc acaccatacg
    18361 cacgtaccgg caactggccg ctgtgggtgc tgacggcact gtttggtttt gctgctgtgt
    18421 tgatgagtct gcgtcagcga cgtaaataat cccttcttat cgtgccggaa tacatcccat
    18481 atttcggcac gctcaccgga tgcggcatca gtgcattatc cggataacaa tattctccta
    18541 tccaactttg gcacatctat tgctttgtta tacaaggcaa agctttaacc agcatcagta
    18601 caacccggtt gcaccacagc aatccaccgg tagcaccaaa agagtgcatt ttaagaattt
    18661 tgcttcggaa tggtgcggcg cacctcccaa atataaacaa taaaacagca atatctttac
    18721 atttagccaa actaaatgtt acctaacagg cacaacacta cacaataaag ttgcagatga
    18781 taacaacaca aacactcaca acgggtatcc atgcgttctt aacgcagaag ataaaggagt
    18841 tggatatgca attacgtaaa cctgccacag caatcctggc cctggcgctt tccgcaggac
    18901 tggcacaggc agatgacgcc gccccggcag cgggcagtac gctggacaaa atcgccaaaa
    18961 acggcgtgat tgtcgtcggt caccgtgaat cttcagtacc tttctcttat tacgacaatc
    19021 agcaaaaagt ggtgggttat tcgcaggatt actccaacgc cattgttgaa gcagtgaaaa
    19081 agaaactcaa caaaccggac ttgcaggtga aactgattcc gattacctca caaaaccgta
    19141 ttccgctgct gcaaaacggc acttttgatt ttgaatgtgg ttctaccacc aacaacgtcg
    19201 aacgccaaaa acaggcggct ttctctgaca ctattttcgt ggtcggtacg cgcctgttga
    19261 ccaaaaaggg cggcgatatc aaagattttg ccgacctgaa aggcaaagcc gtagtcgtca
    19321 cttccggcac tacctctgaa gttttgctca acaaactgaa tgaagagcaa aaaatgaata
    19381 tgcgcatcat cagcgccaaa gatcacggtg actctttccg taccctggaa agcggtcgtg
    19441 ccgttgcctt tatgatggat gatgctctgc tggccggtga acgtgcgaaa gcgaagaaac
    19501 cagacaactg ggatatcgtc ggcaagccgc agtctcagga agcctatggt tgcatgttgc
    19561 gtaaagacga tccgcagttc aaaaagctga tggatgacac tatcgctcag gtgcagacct
    19621 ccggtgaagc ggaaaaatgg tttgataaat ggttcaaaaa tcccattccg ccgaaaaacc
    19681 tgaacatgaa tttcgaactg tcagacgaaa tgaaagcact gttcaaagaa ccgaatgaca
    19741 aagcactgaa ctaattacaa gaaccagggg cggaaattct agccctctcg attgttacgt
    19801 agcacggaca gactatacgc ctgatggtcg ttccccatcg ggcctgaaaa ccgcaatacg
    19861 ctgggtaaca atcttcgagg gtagcagtta acgctgctac cctctttttt ctggagtaga
    19921 tttatgtcta tagactggaa ctggggtatt tttttacaac aagccccgtt cggcaacacc
    19981 acctatcttg gttggatctg gagtggtttt caggtgacga tcgctttgtc gatctgcgcc
    20041 tggattatcg ctttcctcgt cggttcattt ttcggtattt tacgtaccgt tccaaaccgt
    20101 tttctctccg gtctgggtac gttgtatgtc gaactgttcc gcaacgtgcc gttgattgtg
    20161 caattcttta cctggtatct ggtgatcccg gagctgctgc cggagaaaat cggcatgtgg
    20221 tttaaggccg agctggatcc caatattcag tttttccttt catccatgct ctgcctgggg
    20281 ctgtttaccg ccgcccgtgt ttgcgaacag gtacgcgccg cgattcagtc gctgccgcgt
    20341 gggcagaaga atgccgcgct ggctatgggt ctgacgctgc cgcaggctta ccgctacgta
    20401 ctgctgccta atgcttatcg cgttatcgtc ccgccgatga cctcagagat gatgaacctg
    20461 gtgaaaaact ccgccatcgc ctcgaccatc ggtctggtag atatggcagc gcaggcgagt
    20521 aaattgctcg attactcggc tcacgcctgg gagtcgttta ccgccattac gctagcgtat
    20581 gtgtttatta acgcttttat catgctggtg atgacgctgg ttgaacgtaa agttcgcctg
    20641 cctggcaaca tggggggcaa ataatgtacg agtttgactg gagttccatt gtcccttccc
    20701 tgccatatct gctcgacggg ctggtgatca cgctgaaaat caccgtcacg gcagtcgtga
    20761 ttggtatttt gtggggcacc atgctggcgg tgatgcgctt gtccagcttt gcgcccgtag
    20821 cgtggtttgc caaagcctac gttaacgtat tccgctcgat tcctttagtt atggttttgc
    20881 tgtggtttta cctgatcgtg ccgggttttc tgcaaaacgt gctgggatta tcaccaaaaa
    20941 atgatatccg tttaatttcg gcgatggtgg cgttttcgat gtttgaagcg gcctactatt
    21001 cagagattat ccgtgccggt attcaaagta tttctcgtgg tcagtcgagc gccgcgctgg
    21061 cgttgggaat gactcactgg cagtcgatga aactgattat tctgccgcag gcgttccgcg
    21121 cgatggtgcc gctgctgctc actcagggca tcgtactgtt ccaggatacc tcgctggtgt
    21181 atgtgttatc tctggccgat ttcttccgta ccgcctcaac cattggtgag cgtgatggca
    21241 cgcaggttga gatgatcctg tttgccggat ttgtttattt cgttattagc cttagcgcgt
    21301 cgttgttggt cagctacttg aaaagaagga cagcataatg attaccctga aaaatgtttc
    21361 aaaatggtat ggtcactttc aggtgctgac cgactgctca accgaagtga aaaaaggcga
    21421 agtggtggtg gtttgcggcc cgtctggttc cggcaaatca acgctgatta aaaccgtcaa
    21481 cggcctcgaa ccagtgcagc aaggtgaaat caccgtcgat ggtatcgtgg ttaacgacaa
    21541 gaaaaccgat ctggcaaagc tgcgttctcg cgtcgggatg gtattccaac atttcgagct
    21601 gttccctcat ctgtcgatta tcgaaaacct gaccctggcg caggtgaaag tgcttaaacg
    21661 cgataaagca ccggctcgtg aaaaagcatt gaaattgctg gagcgcgtgg gactttccgc
    21721 acatgccaat aaatttccgg cgcagctttc cggcggccag cagcagcgtg tggcaattgc
    21781 tcgcgcattg tgtatggatc ctattgcgat gctgttcgac gaacccacat cggcactaga
    21841 cccggagatg atcaacgaag tactggacgt gatggtggaa ctggcgaacg aggggatgac
    21901 catgatggtg gtgacccacg aaatgggctt tgcccgtaaa gtggcgaatc gggtgatctt
    21961 tatggatgag ggtaaaattg ttgaagactc gccgaaagat gcgttcttcg atgatccgaa
    22021 atcggaccgc gcaaaagact tcctcgcgaa aatcctgcat taatcactct ggcgcgcata
    22081 cccaagtgcg cgccatctca cgctttatcg tctcgcccct cgtatctgtt acatcctggc
    22141 gttacccttg tcgcaaagaa gcacacaaca aggagcaaca atggcactgc caattctgtt
    22201 agattgcgac ccaggtcatg atgacgctat cgcaatagtt ctcgccctcg cctcaccaga
    22261 gcttgatgtc aaagcaatta cgtcttccgc cggaaaccag acaccagaaa aaaccttacg
    22321 caatgttctg cgtatgctga ccttgcttaa tcgcaccgat attccggtgg caggcggcgc
    22381 ggtaaaaccg ttaatgcgtg agttgattat cgcggacaat gtgcacggcg aaagcggtct
    22441 cgacggcccg gcattaccgg aaccgacgtt cgcaccgcaa aactgtacgg cggtagagct
    22501 gatggcgaaa acgctgcgtg aaagtgcgga acctgtcacg attgtgtcta ccggaccgca
    22561 aactaacgtt gccttgctgc tcaatagcca tccggaactg catagcaaaa ttgcccgtat
    22621 cgtgattatg ggtggcgcta tgggactggg taactggacg cctgcggctg aatttaatat
    22681 ttacgttgac ccggaagcgg cagaaattgt cttccagtca gggatcccgg tggtgatggc
    22741 cggtctggat gttactcaca aagcacaaat ccacgttgaa gacactgagc gtttccgcgc
    22801 gattggcaac cctgtttcaa ccattgttgc cgaactgctg gatttcttcc tcgaatatca
    22861 taaagacgaa aaatggggct ttgtcggcgc accgctacat gacccatgca ccatcgcctg
    22921 gctgttaaaa ccggagttgt ttaccactgt tgagcgctgg gttggcgtgg aaacacaggg
    22981 gaaatatacc cagggtatga cggttgttga ttattattat ctgactggca ataaaccgaa
    23041 tgccactgta atggtcgatg ttgatcgtca gggctttgtt gatttactgg ctgatcgtct
    23101 gaaattttac gcttaacaca cgtcattgat taagatcacc acgcgttacc tgggtggtct
    23161 taatctgttt atctcttttt ctttcaagat ataaatgcaa tggataatgc agaactcgcc
    23221 attggtatcg atctcggtac taccaatagt ttaattgccg tctggaaaga cggtgccgcg
    23281 caattaattc caaataagtt cggtgaatat ttaacaccat ccataattag catggatgaa
    23341 aataatcata ttttagtcgg aaaaccggct gtatcacggc gtacttcgca tccggataaa
    23401 acggcagcgt tatttaaacg tgcaatgggc agtaatacca actggcggtt aggcagcgac
    23461 acatttaacg cgccagaact gtcctctttg gtattacgct cattaaaaga agatgccgaa
    23521 gaatttctgc aacgtccgat taaagatgtg gtgatctctg ttccggctta tttcagcgat
    23581 gaacaacgca agcatacccg tttagcagcg gagttagccg gtttaaatgc ggtacgctta
    23641 attaatgaac ccacagcagc tgcgatggcg tatggcctgc atacccaaca aaatacccgt
    23701 tcgctggtgt ttgatctcgg tggcggcacg tttgacgtca cggtgcttga gtacgccacg
    23761 ccggtgattg aagttcacgc ctccgctggc gacaacttcc tcggtggcga agattttacc
    23821 catatgctgg tcgatgaggt tttaaaacgc gcggatgtcg ccaggaccac gcttaacgag
    23881 agtgaactgg cagccttgta tgcctgtgtg gaagcggcaa aatgtagcaa tcaatcgcca
    23941 atacacattc gctggcagta ccaggatgaa acgcgggaat gcgaatttta cgagaacgaa
    24001 ctggaagatt tgtggttgcc gctgctcaat cgcttgcgag tgccgattga acaggcgttg
    24061 cgcgatgcgc gtctgaagcc gagtcaaatc gacagtctgg tgctggttgg cggcgcgtca
    24121 caaatgccgc tggtgcagcg aatcgccgtg cgtctgtttg gcaaattacc gtatcaaagt
    24181 tacgatccga gcaccattgt cgcgctgggc gcagcaatcc aggcagcctg ccgcttacgc
    24241 agtgaagata ttgaagaagt gatcctcact gatatttgcc cttactcgtt gggcgttgag
    24301 gttaaccgcc agggcgtttc cggcattttc tcgccgatta ttgaacgaaa caccactgtg
    24361 cccgtgtcgc gtgtagaaac ttattcaacc atgcacccgg aacaggattc tattacggtt
    24421 aacgtctatc agggagaaaa ccacaaagtt aaaaacaaca ttctggtgga atccttcgat
    24481 gtgccgttga agagaaccgg ggcttatcag tcgattgata ttcgctttag ttatgatatc
    24541 aacggtttgc ttgaagttga cgtgcttctg gaagacggca gcgttaagtc cagagtgatt
    24601 aaccacagcc cggtaacatt gagcgcgcag cagattgaag agagtcggac gcggttatcc
    24661 gcattgaaaa tttatccgcg cgatatgctc atcaaccgca cctttaaggc caaactggaa
    24721 gagttgtggg cgcgggcgct gggtgacgag cgagaagaga ttggccgggt gatcaccgat
    24781 tttgatgcgg cgctgcagtc aaacgatatg gcccgcgtcg atgaggtcag gcggcaggcg
    24841 agcgtttatt tagccattga gatcccataa tcacggctgc cggatgcggc gtaaatgctg
    24901 catccggcat cggcgcaaaa aacacatcgt caactaaaca acatcgcgat tttgccgaga
    24961 gcataaccta tcaccagcac cgttaagctg attttgataa tgctggtaat gctctgtggt
    25021 ttttcatcct catcattttt ttcttcatgg ttttcaggtg ttggctcatc gacatcttca
    25081 tcctccggct cgagttttac tttcgtcaac agcatccggg tgataatggc ctctaatgga
    25141 tgagcatctc cgccttccag ccttgtatta agacgggcca gcatctcttg tttcggaaac
    25201 gcttcaagta ctacctgttc cgctacccga tacaaatcca gtaacggtga atcttcttct
    25261 gtctcgctac cgactctcga tttccaccac gcccatgctt tgcgtttgaa gaatagcgac
    25321 tcaataaacg attgacctaa cggcgatagt tttccgctat gtaaaatttc cgcccataac
    25381 agacgcgtcc gggcgctctc accaggaaca tatttcaacg gtttaccttg cgcatctacc
    25441 atgctccgcg cttcgataac catcactaat aacgcgaaat aatccgggca atgttgtgcg
    25501 ctccactgca acaacaaatt atcggcttgt gtagatgggt aacttcccca gtacgcgcag
    25561 agatcggtaa gcaggctttc cccctcgccg cagtacaaac gttgtgcaca ctggtaatac
    25621 cgggcatctt cgctttctgg ttcttccatt tgccactgtt cggctacagg aatgagttcc
    25681 gccatacctg tttgcaccga gctgtaccag cgcaacagtt tgcgatgtaa ctttgcatca
    25741 tcaggaatga accacggacc ttccatcgcc aaaaacgcag tgacatagtc aggatggtaa
    25801 cggcagcaac gttccagcgc gaagtaatac tcaacagtct ggttctgcaa cgcaacggga
    25861 tataccagaa ggccaaggaa atcgaaacaa tcaccagcct gaatggcctc aagaaaagcc
    25921 tccctttctt actcatctat tccttcgctg tcattaaacg aatgccagtt caaacgctct
    25981 gccagcaaag aagcacaatt taatgaaaga tatcgcgctt ctatagcgat tgcacacagc
    26041 ggccagcgta attgatcgac atcctccatg ttccaggtat ttaattgctg aataaatttc
    26101 tgccaggcgg agggctgaaa acgatcactt tctgaatcca gtaatgccct gaatacacgt
    26161 agtatttcat gctcagtggc atcatctttt tcttcatcat ctgcttcctc gacaggattt
    26221 accgccagtc gcagggcctc ttcataggcc tggcgtaatg ctttaaaccc ttgcggatcg
    26281 gtttcgggat gacataacgg taagcgagca agataagcct ggcggataat gtctatttgc
    26341 gtcgtgcttt caatttcgag gatttgccaa catgttttca taggtcgctc acatccttaa
    26401 gcgttaccgg gcattcaggc tggacagtta atggctgcca gggaagcgtg gcatagaaac
    26461 gatcgcttcc cgcagccagc tcttcgaaca tccgactccc ggcgctatcg tatttgtaca
    26521 gggttagcgc ctcgcgtagc gaagcgttgt cttcgcattg agaagattgc cagtagagac
    26581 gccctagtga ataggcggaa aaatagtgca tccagctgtg atagtaatgg tgagctcgtt
    26641 catagacgcg cgattgcagc cataaactct cgtcttccgt cagccacttg ttgagcacac
    26701 cgacccgtga cagaaacccc attcgaacat aatcccaagc tttgattccc ccttccccac
    26761 aacacatagc ggtttgggca acgaattggg tataaatcaa ccctctctcc gagccaccag
    26821 caattaatgc tttccactct tcaggggaag agcgatgcca tgtcagatat aagccagcta
    26881 aatcattggc atgaccatca tcagtcatct gataaatagt ctgaattaaa tcatcacgat
    26941 tatttattcc ccaggatgat gagagatcga attcttttat cccggtatac atttttggcg
    27001 agtcatgcgc gggtttgcaa ccctgattaa gtatttccat gggcgcagaa agcgcaaaaa
    27061 gaagatattg ttcttcctta ttcatttgtt aataatccct taagtgcatt aactgactac
    27121 ttttttatta ttgatgagta taaacttgac gctttcaaaa taa
//
LOCUS       DAZFAB010000057        26574 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZFAB010000057 DAZFAB010000000
VERSION     DAZFAB010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26574
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.057"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..1340)
                     /gene="djlB"
                     /locus_tag="ACHJWA_002747"
     CDS             complement(<1..1340)
                     /gene="djlB"
                     /locus_tag="ACHJWA_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFO4309308.1"
                     /translation="MKNCWKILNIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMNEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQIILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWHEYPSTQADALMLQWCRQHRVDYYPLVVMMIEARELVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGCKPRISLTRDTEHTLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAENRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDDGG"
     gene            complement(1337..2044)
                     /gene="ybeR"
                     /locus_tag="ACHJWA_002748"
     CDS             complement(1337..2044)
                     /gene="ybeR"
                     /locus_tag="ACHJWA_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO4309309.1"
                     /translation="MDMESQKILFALSTPMEIRNECCLPSHSSPKMYLGTRFFDLSSS
                     WGIDDRDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDNLPLLREFLARKEYDDSGNDMFYQLFASDDAYYATLPWQP
                     LADYPTCPETLKDMSDL"
     gene            2209..3186
                     /gene="ybeQ"
                     /locus_tag="ACHJWA_002749"
                     /pseudo
     CDS             2209..3186
                     /gene="ybeQ"
                     /locus_tag="ACHJWA_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
     gene            complement(3256..3738)
                     /gene="ybeL"
                     /locus_tag="ACHJWA_002750"
     CDS             complement(3256..3738)
                     /gene="ybeL"
                     /locus_tag="ACHJWA_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFO4309310.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            3974..6556
                     /gene="leuS"
                     /locus_tag="ACHJWA_002751"
     CDS             3974..6556
                     /gene="leuS"
                     /locus_tag="ACHJWA_002751"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFO4309311.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVNTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPQWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            6571..7152
                     /gene="lptE"
                     /locus_tag="ACHJWA_002752"
     CDS             6571..7152
                     /gene="lptE"
                     /locus_tag="ACHJWA_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFO4309312.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGAVSISQDTASVFRNGQTAEYQMVM
                     TVSASVLIPGRDIYPISTKVFRSFFDNPQMALAKDNEQEMIIKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            7152..8183
                     /gene="holA"
                     /locus_tag="ACHJWA_002753"
     CDS             7152..8183
                     /gene="holA"
                     /locus_tag="ACHJWA_002753"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFO4309313.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYSQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            8185..8826
                     /gene="nadD"
                     /locus_tag="ACHJWA_002754"
     CDS             8185..8826
                     /gene="nadD"
                     /locus_tag="ACHJWA_002754"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFO4309314.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCDDLLPEPVLTYINQQGLYR"
     gene            8850..9461
                     /gene="cobC"
                     /locus_tag="ACHJWA_002755"
     CDS             8850..9461
                     /gene="cobC"
                     /locus_tag="ACHJWA_002755"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFO4309315.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            9721..10038
                     /gene="rsfS"
                     /locus_tag="ACHJWA_002756"
     CDS             9721..10038
                     /gene="rsfS"
                     /locus_tag="ACHJWA_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFO4309316.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10042..10509
                     /gene="rlmH"
                     /locus_tag="ACHJWA_002757"
     CDS             10042..10509
                     /gene="rlmH"
                     /locus_tag="ACHJWA_002757"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFO4309317.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            10540..12441
                     /gene="mrdA"
                     /locus_tag="ACHJWA_002758"
     CDS             10540..12441
                     /gene="mrdA"
                     /locus_tag="ACHJWA_002758"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFO4309318.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            12444..13556
                     /gene="mrdB"
                     /locus_tag="ACHJWA_002759"
                     /gene_synonym="rodA"
     CDS             12444..13556
                     /gene="mrdB"
                     /locus_tag="ACHJWA_002759"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFO4309319.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            13567..14655
                     /gene="rlpA"
                     /locus_tag="ACHJWA_002760"
     CDS             13567..14655
                     /gene="rlpA"
                     /locus_tag="ACHJWA_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFO4309320.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFIQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FANKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            14795..16006
                     /gene="dacA"
                     /locus_tag="ACHJWA_002761"
     CDS             14795..16006
                     /gene="dacA"
                     /locus_tag="ACHJWA_002761"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFO4309321.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16116..16379
                     /gene="ybeD"
                     /locus_tag="ACHJWA_002762"
     CDS             16116..16379
                     /gene="ybeD"
                     /locus_tag="ACHJWA_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFO4309322.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            16480..17121
                     /gene="lipB"
                     /locus_tag="ACHJWA_002763"
     CDS             16480..17121
                     /gene="lipB"
                     /locus_tag="ACHJWA_002763"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFO4309323.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            17381..18334
                     /gene="ybeF"
                     /locus_tag="ACHJWA_002764"
     CDS             17381..18334
                     /gene="ybeF"
                     /locus_tag="ACHJWA_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFO4309324.1"
                     /translation="MDSNNQIESCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRIQEMFPDRQISFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            18543..19508
                     /gene="lipA"
                     /locus_tag="ACHJWA_002765"
     CDS             18543..19508
                     /gene="lipA"
                     /locus_tag="ACHJWA_002765"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFO4309325.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(19609..19812)
                     /gene="tatE"
                     /locus_tag="ACHJWA_002766"
     CDS             complement(19609..19812)
                     /gene="tatE"
                     /locus_tag="ACHJWA_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFO4309326.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(19941..20729)
                     /locus_tag="ACHJWA_002767"
     CDS             complement(19941..20729)
                     /locus_tag="ACHJWA_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFO4309327.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            20822..21205
                     /gene="crcB"
                     /locus_tag="ACHJWA_002768"
     CDS             20822..21205
                     /gene="crcB"
                     /locus_tag="ACHJWA_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFO4309328.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(21259..21468)
                     /gene="cspE"
                     /locus_tag="ACHJWA_002769"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(21259..21468)
                     /gene="cspE"
                     /locus_tag="ACHJWA_002769"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO4309329.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(21643..22203)
                     /gene="pagP"
                     /locus_tag="ACHJWA_002770"
     CDS             complement(21643..22203)
                     /gene="pagP"
                     /locus_tag="ACHJWA_002770"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFO4309330.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIVQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            22792..24177
                     /gene="dcuC"
                     /locus_tag="ACHJWA_002771"
     CDS             22792..24177
                     /gene="dcuC"
                     /locus_tag="ACHJWA_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO4309331.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(24218..24898)
                     /gene="dpiA"
                     /locus_tag="ACHJWA_002772"
                     /gene_synonym="citB"
     CDS             complement(24218..24898)
                     /gene="dpiA"
                     /locus_tag="ACHJWA_002772"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFO4309332.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(24867..26525)
                     /gene="dpiB"
                     /locus_tag="ACHJWA_002773"
     CDS             complement(24867..26525)
                     /gene="dpiB"
                     /locus_tag="ACHJWA_002773"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFO4309333.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISEVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
BASE COUNT         6707 a         6688 c         6512 g         6667 t
ORIGIN      
        1 ccaccatcgt caacgggcat tggctgcggt tgcggctccg gttcaacagt ttgcttctcg
       61 gtaaaattcg ccgcctgaat taataatcga cgaataatta atgcttccag tggacaacga
      121 ttttctgcat caagacggct tacgatcggc attaatgatt cttctgtagg ttggctggcc
      181 tgcactaatt gtttggcaac tagatataat ggccataagg tatgttctgt atcacgcgtg
      241 agtgagatcc gcggcttaca tcctttgtgc aaaaccatct cgaccagtga acgccccaga
      301 gcagagagtt tttcatcgct gagtatttcg tataaatgaa aacgcgtacg ggcgctgtcg
      361 ccggggacat aaagtagcgg ttttccctgg tcgttaacca gttcacgagc ttcaatcatc
      421 atcaccacta atgggtaata atcgactcga tgctgacggc accattgcaa cattaaagca
      481 tccgcctgag tggagggata ctcgtgccag tagtcacaaa gttctgccag caggctttcg
      541 ccttctccac aatagacgcg ttgcgcgtat tcataataac gcgcatcttc attatcaggt
      601 tcagcttccc gccacgaccg ggcgtaatcc aggagttcgg ggattcccag gcgacctgca
      661 ctataccagc ggagtaaatt tctgtgcagt tttgcatcat cggggataat cactgcgcga
      721 tgttgcgcta aatacaatgt cagccattct ggatgcgatg accaaatccg ggcgaacatt
      781 tggtaaaaat cgatggtgtc attttgcacg gcaacgggca gatgcagaat gatctggaag
      841 ttaaaaacat ttcccttcgc aatggcatat aaaaaggatt ccagttcttc ttcgtctatt
      901 tccccgacgt tttcctcctg caaccaccgc aatctttctg ctaataacac cacgcactcg
      961 aaggataaat gggcagtgtt catggctatt gtgcacagcg accagcgtaa ttcatcaatc
     1021 tcattcatcg agcaataatt taattgctga atgaatcgct gccaggcgga gggcagaaaa
     1081 cgttcactat cagaggcaag taacgcacga aaggcgagca gaatttcatg ttctgctacc
     1141 tcatattctt ctggttgcca aacagattta gcaggcgact gcgcaatccg gagcgcttcc
     1201 tcatacgcct gacgaagttg tttaaaaccc tgcggatcgg tttctggatg aaaggacggt
     1261 aacagcgcca gataagcgcg gcggataata tcgacatcag tcgtttcctc tatgttgagg
     1321 atcttccagc aatttttcat aggtcgctca tatccttaag cgtttccggg catgtggggt
     1381 agtcagccag tggttgccag ggtaaggtcg cgtaatatgc atcgtcactg gcaaataatt
     1441 gataaaacat atcattgccg gaatcgtcgt attctttacg cgctaagaat tcacgtaata
     1501 aaggcagatt atcatcttcg ggagactgcc aatattgccg accaaaggta taaccggcaa
     1561 agtattgccg ccagttacta taataacgta gagcccgtag atgaatacgc gactggatcc
     1621 atagactttc ttcttcactc aaccagttat taagtacgcc cattcgactt aaaaaaccca
     1681 tccggacata atcccatgct ttaattcctc cctcaccaca gcattcggct gtgctggcga
     1741 caaattgcgc ataagcctga ccttgctcat ttaattcagc aagatagtca cgccattcac
     1801 atggagaata acgaaaccag cggtgataaa accctgccaa ccgcgcggca tgaccgttat
     1861 cgatcattcg gtgaattgtg cgcagcaggt catcacggtc atcaattccc caggaggaag
     1921 aaaggtcaaa aaagcgtgta cctaaataca ttttgggtga tgaatgcgaa ggtaaacagc
     1981 attcattgcg tatttccatc ggagtggaaa gtgcaaacaa tattttttgc gactccatgt
     2041 ccatatgatt tatccttaaa tattcattga atgaatatta gccgcattgc gacgctcgtt
     2101 tatttatact tttctgacgc gacataatac gcataaaata aggcttgcgc aaaaaatgaa
     2161 acacataaaa attcaatata ttttatctac aatcataagg aaatgataat gattttcacg
     2221 tcaagttgct gcgataattt atcaatagat gagattatcg aacgtgctga aaaaggcgat
     2281 tgcgaggctc aatatattgt tgggttttat tataatcgcg atagcgcaat tgactctccg
     2341 gacgacgaaa aggcctttta ctagctgaag ctggccgctg agcaaggtca ttgtgaagca
     2401 cagtattcct tagggcaaaa gtataccgag gataaaagcc gtcataaaga taatgagcaa
     2461 gccatcttct ggctgaaaaa agctgcccta caaggccata ctttcgcttc caacgccctt
     2521 ggctggatac tggatcgtgg agaagacccc aactataaag aagcggttgc ctggtatcag
     2581 atagccgcgg agagcggaat gtcttatgcg caaaataatc ttgggtggat gtacagaaat
     2641 ggcaacggag ccgcacaaga ctatacgctg gcatttttct ggtacaaaca agctgcatta
     2701 caaggccata gtgacgcgca aaacaatctg gccgatcttt atgaagacgg aaaaggcgtt
     2761 gctcaaaacg agacactcgc cgcattctgg tatttgaaaa gcgcacagca gggtaatcgg
     2821 catgctcagt ttcaaattgc atgggattat aacgctggcg aaggggtgga ccaggactat
     2881 aagcaagcga tgtactggta tctgaaggct gccgctcagg ggagcgtcgg cgcttacgtc
     2941 aacatcggtt atatgtataa acacggacaa ggcgttgaga aagattatca ggctgccttt
     3001 gaatggttta cgaaagccgc tgaatgcaat gacgccactg cctggtataa cctggccatt
     3061 atgtatcact acggagaagg aagacctgtc gatctccgac aggctctcga cctgtatcgt
     3121 aaagttcagt catccggaac cagagatgtc agtcaggaaa ttcgtgagac tgaagattta
     3181 ctgtagagat aaaaacaagg gccggggaat ttcattcggc ccatattctc tttattgctg
     3241 tgcgaaatca gactattacg gctcaaacgg gcgtctctgg aactggtcat gaccacattt
     3301 cgggcatagc gtcagcactt ccggtgtgta gatcgggaga tggaagtgac atttctcgca
     3361 gaccagattt cccagcccga ccacttctcc gctgtgataa accccatgat gattgaggtc
     3421 ctggaaaact tcgcgccatt caagctgcgt tttatcggtg atgtctgcca gctcctgcca
     3481 caagctttct ttaatcaccc gcataaagac gctgtcagat tcttctttca ggctctcttc
     3541 atagctcatg gcgaactctt ccaggtcacg tctgacagct cgcgtcagct catcgacctc
     3601 ggttcgcgtt aactcccctg tttttattac gcgctcgcgc gcctgttcca ccagtgcgtc
     3661 gatatcacgt tcgccattgc gcaggcgttc gctcagtgac gcaaccagtt cacggtaata
     3721 ttgagcaacc ttgttcatcg tttcatctcc cgggtcgtta actgactata ataatagacg
     3781 ttctttgttc cttgctttgc taatacgccc acaaactctg taaattaatg tatgaataat
     3841 ccgggcatca tttcaacgtc gagtgcgcga aaggctgttt tgacgcggac gtttgggcta
     3901 tgctatgcgg atctgaaaaa ccacctctaa cgctactttt gtagccgtaa tgaaaacagg
     3961 accactggct gccatgcaag agcaataccg cccggaagag atagaatcca aagtacagct
     4021 tcattgggat gagaagcgca catttgaagt aaccgaagac gagagcaaag agaagtatta
     4081 ctgcctgtct atgcttccct atccttctgg tcgactacac atgggccacg tacgtaacta
     4141 caccatcggt gacgtgatcg cccgttacca gcgcatgctg ggcaaaaacg ttctgcagcc
     4201 gatcggctgg gacgcgtttg gtctgcctgc ggaaggcgcg gcggtgaaaa acaacaccgc
     4261 tccggcaccg tggacgtacg acaacatcgc gtatatgaaa aaccagctca aaatgctggg
     4321 ctttggttat gactggagcc gcgagctggc aacctgtacg ccggaatact accgttggga
     4381 acagaaattc ttcaccgagc tgtataaaaa aggcctggta tataagaaga cttctgcggt
     4441 caactggtgc ccgaacgacc agaccgtact ggcgaacgaa caagttatcg acggctgctg
     4501 ctggcgctgc gataccaaag ttgaacgtaa agagatcccg cagtggttta tcaaaatcac
     4561 tgcttacgct gacgagctgc tcaacgattt ggataaactg gatcactggc cagacaccgt
     4621 taaaaccatg cagcgtaact ggatcggtcg ttccgaaggc gtggaaatca ccttcaacgt
     4681 taacgactat gacaacacgc tgaccgttta cactacccgc ccggacacct ttatgggttg
     4741 tacctacctg gcggtagctg cgggtcatcc gctggcgcag aaagcggcgg aaaataatcc
     4801 tgaactggcg gcctttattg acgaatgccg taacaccaaa gttgctgaag ctgaaatggc
     4861 gacgatggag aaaaaaggcg tcaatactgg ctttaaagct gttcacccat taacgggcga
     4921 agaaattccc gtttgggcag caaacttcgt attgatggag tacggcacgg gcgcagtaat
     4981 ggcggttccg gggcacgacc aacgcgacta cgagtttgcc tctaaatacg gcctgaacat
     5041 caaaccggtt atcctggcgg ctgacggctc tgagccagat ctttctcagc aagccctgac
     5101 tgaaaaaggc gtgctgttca actctggcga gttcaacggt cttgaccatg aagcggcctt
     5161 caacgccatc gccgataaac tgactgcgat gggcgttggc gagcgtaaag tgaactaccg
     5221 cctgcgtgac tggggtgttt ctcgtcagcg ttactggggc gcgccgattc cgatggtgac
     5281 gctggaagac ggtaccgtaa tgccgacccc ggacgaccag ctgccggtga tcctgccgga
     5341 agatgtggtg atggacggca ttaccagccc gattaaagca gatccacagt gggcgaaaac
     5401 taccgttaac ggtatgccag cgctgcgtga aaccgacact ttcgacacct ttatggagtc
     5461 ctcctggtac tatgcgcgct acacttgccc agagtacaaa gaaggtatgc tggattccga
     5521 agcggctaac tactggctgc cggtggatat ctacattggt ggtatcgaac acgccattat
     5581 gcacctgctc tacttccgct tcttccacaa actgatgcgt gatgcaggca tggtgaactc
     5641 tgacgaacca gcgaaacagt tgctgtgtca gggtatggtg ctggcagatg ccttctacta
     5701 tgttggcgaa aacggcgaac gtaactgggt ttccccggtt gatgctatcg ttgaacgtga
     5761 cgagaaaggc cgtatcgtga aagcgaaaga tgcggcaggc catgaactgg tttataccgg
     5821 catgagcaaa atgtccaagt cgaagaacaa cggtatcgac ccgcaggtga tggttgaacg
     5881 ttacggcgcg gacaccgttc gtctgtttat gatgtttgct tctccggctg atatgactct
     5941 cgaatggcag gaatccggcg tggaaggggc taaccgcttc ctgaaacgtg tctggaaact
     6001 ggtgtacgag catactgcga aaggtgatgt tgcggcactg aacgttgatg cactgactga
     6061 agatcagaaa gcgctgcgtc gcgatgtgca taaaactatc gctaaagtga ccgatgatat
     6121 cggccgtcgt cagaccttca acaccgcaat tgcggcgatt atggagctga tgaacaaact
     6181 ggcgaaagca ccgaccgatg gcgagcagga tcgcgctctg atgcaggaag cactgctggc
     6241 cgttgtccgt atgcttaacc cgttcacccc gcacatctgc ttcacgctgt ggcaggaact
     6301 gaaaggcgaa ggcgatatcg acaacgcgcc gtggccggtt gctgacgaaa aagcgatggt
     6361 ggaagactcc acgctggtcg tggtgcaggt taacggtaaa gtccgtgcta aaatcaccgt
     6421 tccggtggac gcaacggaag aacaggttcg cgaacgtgct ggccaggaac atctggtagc
     6481 aaaatatctt gatggcgtta ctgtacgtaa agtgatttac gtaccaggta aactcctcaa
     6541 tctggtcgtt ggctaagcgc gggaggaagc gtgcgatatc tggcaacatt gttgttatct
     6601 ctggcggtgt taatcaccgc cgggtgtggc tggcatctgc gtgacaccac gcaggttcct
     6661 tccactatga aggtcatgat cctggactca ggcgatccga acgggccatt aagccgtgcg
     6721 gtgcgtaacc agttacgtct gaatggtgtc gagttgcttg ataaagaaac cacgcgtaag
     6781 gacgttccat ccctgcgttt gggtgctgta tcaatctcac aggataccgc gtcggttttc
     6841 cgtaatggtc aaactgcgga atatcagatg gtgatgactg ttagcgcgtc ggtattgatt
     6901 cctggacgag atatctaccc gattagcacc aaagtcttcc gttcattctt cgataacccg
     6961 caaatggcgt tagcgaaaga taacgagcag gaaatgatca ttaaagagat gtacgaccgt
     7021 gctgccgaac agctgattcg taagctgcca agcatccgtg ctgcggatat tcgttccgac
     7081 gaagaacaga cgtcgactac aacggatact ccggcaacgc ctgcacgcgt ctccaccacg
     7141 ctgggtaact gatgattcgg ttgtacccgg aacaactccg cgcgcagctc aatgaagggc
     7201 tgcgcgcggc gtatctttta cttggtaacg atcctctgtt attgcaggaa agccaggacg
     7261 ctgttcgtca ggtagctgcg gcacaaggat tcgaagaaca ccacactttt tccattgatc
     7321 ccaacactga ctggaatgcg atcttttcgt tatgccaggc catgagtctg tttgccagtc
     7381 gacaaacgct attgctgttg ttaccagaaa acggaccgaa cgcggcgatc aatgaacaac
     7441 ttctcacact caccggactt ctgcatgacg acctgctgtt gatcgtccgc ggtaataaat
     7501 taagcaaagc gcaagaaaat gccgcctggt ttactgctct tgcgaatcgc agcgtgcagg
     7561 tgacctgtca gacaccggag caggctcagc ttccccgctg ggttgctgcg cgcgcaaaac
     7621 agctcaactt agaactggat gacgcggcga atcaggtgct ctgctactgt tatgaaggta
     7681 acctgctggc gctggctcag gcactggagc gtttatcgct gctctggcca gacggcaaac
     7741 tgacattacc gcgcgttgaa caggcggtga atgatgccgc gcatttcact ccttttcatt
     7801 gggttgatgc tttgttgatg ggaaaaagta agcgcgcatt gcatattctt cagcaactgc
     7861 gtctggaagg cagcgaaccg gttattttgt tgcgcacatt acaacgtgaa ctcttgttac
     7921 tggttaacct gaaacgccag tctgcccata cgccactgcg tgcgttgttt gataagcatc
     7981 gggtatggca gaaccgccgg ggcatgatgg gcgaggcgtt aaatcgctta agtcagccgc
     8041 agttacgtca ggccgtgcaa ctcctgacac gaacggaact caccctcaaa caagattaca
     8101 gtcagtcagt gtgggcagag ttggaagggt tatctcttct gttgtgccat aaacccctgg
     8161 cggacgtatt tatcgacggt tgatatgaaa tctttacagg ctctgtttgg cggcaccttt
     8221 gatccggtgc actatggtca tctaaaaccc gtggaaacgc tggcgaattt gattggtctg
     8281 acgcgagtca caatcatccc taataatgtt cctccgcatc gtccccagcc ggaagcgaac
     8341 agcgtacagc gtaaacacat gcttgaactg gcgattgccg acaagccatt atttactctt
     8401 gatgaacgcg agctaaagcg caatgccccc tcttacactg cgcaaacact gaaagagtgg
     8461 cggcaggaac aagggccgga cgtgccgctg gcgtttatta ttggtcagga ttcattgctg
     8521 acctttccga cctggtacga atacgaaacg atactcgaca atgcacattt gatcgtctgt
     8581 cggcgtccag gttacccact tgaaatggcg caaccgcaat accagcaatg gctggaagat
     8641 catttgacac ataacccgga agatcttcac cttcagcctg ccggtaaaat ttatctggct
     8701 gaaacgccgt ggtttaacat ctcggcgacc atcatccgcg aacgtttgca aaacggtgaa
     8761 tcatgtgatg atttattgcc ggaaccggta ctgacttaca ttaaccaaca aggcttgtat
     8821 cgctgatacc tgtcgttacg gagggcatga tgcgactgtg gttaattcgt catggcgaaa
     8881 cacaagcgaa tgtcgatggt ctgtacagcg gtcatgcgcc cacacccctg accgcacgcg
     8941 gtattgagca agcgcaaaat ctgcatacac tgctagatga tgtttccttt gacctggttt
     9001 tatgcagtga actggaacgg gcacagcata ccgcgcgact ggttctcagt gaccgccagc
     9061 tccccgttca tatcatccct gaactcaacg aaatgttttt tggcgactgg gagatgcgac
     9121 atcatcgcga cctcatgcaa gaagatgccg aaaactatag cgcgtggtgc aatgactggc
     9181 agcatgctat ccccacgaac ggtgaaggat ttcaggcatt ttcgcaacgt gtggaacgct
     9241 ttatcgcaag gcttagtgaa tatcagcact atcagaatat tttaatcgtc agccatcagg
     9301 gcgtacttag tctgttaatt gctcgtttaa ttggcatgcc tgccgagtct atgtggcatt
     9361 ttcgcgttga ccaggggtgt tggagcgcca ttgatataaa ccaaaaattc gcgacgctac
     9421 gcgtcctcaa tagccgtgcc atcggggtcg agaatgcatg acttttctgt ttttttacgg
     9481 gtaagccgca acgaccattg acagccccgg gcaggctgat attctccgca gccagacttt
     9541 ttccgccaga cacgactttg tagaaattgt tttacaaaaa tggcgatgca atctgcggcg
     9601 cggggtggga tgatagccca ctttcgaaag ccgattcggc gacaattgtc ccgaaatcgc
     9661 ctctggttca ggtatactga cagaccattt ttatctattt gattcaccca gggggaaaac
     9721 ttgcagggta aagcactcca ggattttgtt atcgacaaaa ttgatgacct caaaggtcag
     9781 gacatcatcg ccttagacgt tcagggcaaa tccagcatca ctgactgcat gatcatctgt
     9841 acgggtacgt ccagccgtca tgttatgtcc attgctgacc acgttgtgca ggagtctcgc
     9901 gcagcgggcc tgttaccgct cggagtagaa ggtgaaaaca gcgccgactg gattgtcgtg
     9961 gatttgggcg atgtgattgt ccatgtcatg caggaagaga gccgtcgcct gtatgaactg
    10021 gaaaaactct ggagttaatg cgtgaagctg caacttgtcg ccgtgggaac gaaaatgccg
    10081 gactgggtac aaaccggttt taccgagtac ctgcgtcgtt ttccgaaaga tatgcccttc
    10141 gagctgattg aaattccggc cggaaaacgc ggcaagaatg cggacatcaa gcgcatactc
    10201 gacaaagagg gtgagcagat gttggcggcc gcaggcaaaa accgcattgt caccctcgat
    10261 attccaggca agccctggga tacgccgcag ttagccgctg agctggaacg ctggaagctg
    10321 gatggtcgcg acgtcagtct actgattggc gggcctgaag ggttgtcgcc tgcctgtaaa
    10381 gcggcggcgg agcagagctg gtcgctgtcg gcgcttaccc tcccccatcc gctggttcgc
    10441 gtgctggtcg cagagagtct gtaccgggcg tggagcatca ccaccaacca tccttatcac
    10501 cgtgagtgat aagggagctt tgagtagaaa acgcagcgga tgaaactaca gaactctttt
    10561 cgcgactata cggcagagtc cgcgctgttt gtgcgccggg cgctggtcgc ctttttgggg
    10621 attttgctgc tgaccggcgt gcttatcgcc aacctgtata atctgcaaat tgttcgcttt
    10681 accgactacc agacccgctc taatgaaaac cgcattaagc tggtgcctat cgcgcccagc
    10741 cgcggcatta tctacgaccg taacggtatc cctctggccc tcaaccgcac tatctaccag
    10801 atagaaatga tgccggagaa agtcgataac gtgcagcaaa cgctggacgc tttgcgcagc
    10861 gtggttgatc tgaccgatga cgatattgct gcattccgaa aagagcgcgc acgttcacac
    10921 cgtttcacct ccattccggt gaaaactaac ctgaccgaag tacaagtagc tcgctttgcc
    10981 gtcaatcagt accgttttcc gggtgttgaa gttaaaggct ataaacgtcg ttactatcct
    11041 tacggttcgg cgttgaccca cgtcatcggc tatgtgtcga aaatcaacga taaagacgtc
    11101 gaacgcctga ataatgacgg caaactggcc aactatgcgg caacgcatga tatcggtaag
    11161 ctgggcattg agcgttacta tgaagacgtg ctgcacggtc agaccggtta tgaagaggtt
    11221 gaagttaaca accgtgggcg tgttatccgc cagttaaaag aagtcccacc gcaagccgga
    11281 cacgatattt acctgacgct ggatctcaaa ctccagcaat atattgaaac gctgctggcg
    11341 ggtagccgcg cagctgtggt agtcaccgat ccgcgtacag gtggggtgct ggcgctggtt
    11401 tccacgccta gttatgaccc aaacttgttt gttgacggta tctccagcaa agattattcc
    11461 gccttgttga acgacccgaa tacaccgctg gtgaaccgcg ccacacaggg ggtttatcct
    11521 cccgcgtcta cagttaaacc ctatgtggcg gtttcggcat tgagcgccgg ggtgatcacg
    11581 cgcaatacga cgctgtttga ccctggctgg tggcaactgc caggttcgga aaaacgttat
    11641 cgtgactgga aaaaatgggg ccacgggcgt ctgaatgtca caagatcgct ggaagaatct
    11701 gcggatacct tcttctatca ggtggcctac gatatgggga tcgatcgcct ctccgaatgg
    11761 atgggtaaat tcggttatgg tcattatacc ggtatcgacc tggcggaaga acgttccggc
    11821 aatatgccta cccgcgaatg gaaacagaaa cgctttaaaa aaccgtggta tcagggtgac
    11881 accattccgg ttggtatcgg tcagggttac tggacagcga ccccaatcca gatgagtaag
    11941 gcactgatga tcctgattaa tgacggtatc gtgaaggttc cgcatttgct gatgagcacc
    12001 gccgaagacg gcaaacaggt gccatgggta caaccgcatg aaccgcccgt tggcgatatt
    12061 cattccggtt actgggagct ggcgaaagac ggtatgtacg gtgttgctaa ccgccctaac
    12121 ggtacggcgc ataaatactt tgccagcgca ccgtacaaaa ttgcggcgaa atctggtacc
    12181 gctcaggtct tcggtctgaa agcgaacgaa acctataatg cgcacaaaat tgccgagcgt
    12241 ttacgtgacc acaaactgat gaccgccttt gcgccataca acaatccgca agtggctgtc
    12301 gccatgattc tggagaacgg tggcgcgggt ccggcggttg gtacgctgat gcgccagatc
    12361 ctcgaccaca ttatgctggg tgataacaac accgatctgc ctgcggaaaa tcctgcggtt
    12421 gccgcagcgg aggaccatta atcatgacgg ataatccgaa taaaaaaaca ttctgggata
    12481 aagtccatct cgatcccaca atgctgctga ttttactggc attgctggtt tacagcgccc
    12541 tggttatctg gagcgccagc ggtcaggata ttggcatgat ggagcgtaaa atcggccaaa
    12601 tcgcgatggg tctggtcatc atggtggtga tggcgcaaat tcctccacgc gtttatgaag
    12661 gctgggcccc ctatctctat atcatctgta ttattttgct ggtggcggta gatgctttcg
    12721 gtgccatctc taaaggcgct caacgctggc tggacctcgg tattgttcgt tttcagccgt
    12781 cggaaattgc caaaatagcc gtaccactga tggttgcgcg ctttatcaac cgcgacgttt
    12841 gcccgccatc gttgaagaac actggcatcg cgctggtgct gatatttatg cccacgctgc
    12901 tggtggctgc acagcctgac ctgggaacat caatcctcgt tgcgctttcc ggtctgtttg
    12961 tactgttcct ctctggcctt agctggcgtc tgattggcgt cgcagtagtg ctggtagcgg
    13021 cgttcattcc gattctgtgg ttcttcctga tgcatgatta ccagcgccag cgcgtaatga
    13081 tgctcctgga cccggaatca gacccactcg gcgcgggcta tcacattatt cagtctaaaa
    13141 ttgctattgg ctccggcgga ttacgcggca aaggctggct gcacggcact cagtcacagc
    13201 ttgaatttct ccccgaacgc catactgact ttatcttcgc ggtactggcg gaagagctgg
    13261 gattagtggg cattttgatt ctgctcgctc tctacattct gctgatcatg cgcgggctgt
    13321 ggatagccgc cagagcgcaa accacctttg gtcgcgtcat ggctggcggc ttaatgctga
    13381 tattattcgt ttatgtcttc gtaaatattg gtatggtaag cggtattctg ccggttgtag
    13441 gggttccgct cccactggtc agttatggag gatcggcgct aattgtgctg atggctgggt
    13501 tcgggattgt aatgtcaatc cacacccaca ggaaaatgtt gtcgaaaagc gtgtaagagg
    13561 tgcgcaatgc gtaagcagtg gctcgggatc tgcatcgcgg caggaatgct cgcggcatgt
    13621 acaagcgatg atggtcagca acagacagta agtgtaccgc agcctgcggt atgtaacggc
    13681 cccatagttg aaattagcgg ggcggacccg cgtttcgaac cactgaacgc gacggcaaat
    13741 caggattacc agcgcgacgg taaaagctac aaaatcgtgc aggatccgtc tcgatttatc
    13801 caggcgggac tggcggcaat ctatgatgcc gaaccaggca gtaacctgac ggcctctggc
    13861 gaagctttcg atccgacaca gctgacggcg gcccatccaa cgcttccgat ccccagctac
    13921 gccagaatca ctaacctggc taacgggcga atgatcgtgg tgcgcattaa tgatcgcggt
    13981 ccttacggca acgaccgcgt tatttcgctt tctcgcgcag cagctgaccg tcttaacacg
    14041 tcaaacaaca ccaaagttcg tatcgatccg attattgtcg cccaggatgg ttcgctttct
    14101 ggtcctggta tggcttgtac cacagtcgcc aaacagactt acgccctgcc tgcacctccc
    14161 gatttaagcg gtggcgcggg aacaagttca gtgtctggcc cgcagggtga cattcttccg
    14221 gtcagtaatt cgacgctaaa aagcgaagat ccgaccggcg cgccggtaac cagcagcggt
    14281 ttcctcggcg caccaacgac cttagcgcca ggtgtactgg aaggcagcga accgacgcct
    14341 gctccacagc ccgttgttac agctccgtcg acaacgcctg caacctcgcc tgcaatggtg
    14401 acaccgcaag ccgcctcgca aagcgccagc ggcaacttta tggtgcaggt cggggccgta
    14461 agcgatcagg ctcgtgcgca acagtaccaa cagcaactgg gacagaagtt cggcgtcccc
    14521 ggtcgcgtaa ctcaaaatgg cgcggtctgg cggatccagc ttggcccatt cgccaacaaa
    14581 gccgaagcca gtaccttgca gcaacgtttg caaaccgaag cccaattaca gtcatttatt
    14641 accaccgcgc agtagcgtaa agcaggcatc tgatgtgtca atttctgtaa atgacgttaa
    14701 caaagtcacg cggaaagtca gatgcctgcc ggtagtgcat ttgctatagt agggcacttt
    14761 ttttaattcc atcacggatg tcgtagttca gaccatgaat accatttttt ccgctcgtat
    14821 catgaagcgc ctggcgctca ccacggctct ttgcacagcc tttatctctg ctgcacatgc
    14881 cgatgacctg aatatcaaaa ctatgatccc gggtgtaccg cagatcgatg cggagtccta
    14941 catcctgatt gactataact ccggcaaagt gctcgccgaa cagaacgcag atgtccgccg
    15001 cgatcctgcc agcctgacca aaatgatgac cagttacgtt atcggccagg caatgaaagc
    15061 cggtaaattt aaagaaactg atttagtcac tattggcaac gacgcatggg ccaccggtaa
    15121 cccggtgttt aaaggttctt cgctgatgtt cctcaaaccg ggcatgcagg ttccggtttc
    15181 tcagctgatc cgcggtataa acctgcaatc gggtaacgat gcttgtgtcg ccatggccga
    15241 ttttgccgct ggtagccagg acgcttttgt tggcttgatg aacagctacg ttaacgccct
    15301 gggcctgaaa aatacccact tccagacggt acatggtctg gatgctgatg gtcagtacag
    15361 ctccgcgcgc gatatggcgc tgatcggcca ggcgttgatc cgtgacgtac cgaatgaata
    15421 ctcgatctat aaagaaaaag aatttacgtt taacggtatt cgccagctga accgtaacgg
    15481 cctgttatgg gataacagcc tgaatgtcga cggcatcaaa accggacaca ctgacaaagc
    15541 aggttacaac cttgttgctt ctgcgactga aggccagatg cgcttgattt ctgcggtgat
    15601 gggcggacgt acttttaaag gccgtgaagc cgaaagtaaa aaactgctaa cctggggctt
    15661 ccgtttcttc gaaaccgtta acccactgaa agtaggtaaa gagttcgcct ctgaaccggt
    15721 ttggtttggt gattctgatc gcgcttcgtt aggggttgat aaagacgtgt acctgaccat
    15781 tccgcgtggt cgcatgaaag atctgaaagc cagctatgtg ctgaacagca gtgaattgca
    15841 tgcgccgctg caaaagaatc aggtcgtcgg aactatcaac ttccagcttg atggcaaaac
    15901 gatcgaacaa cgcccgctgg ttgtgttgca agaaatcccg gaaggtaact tcttcggcaa
    15961 aatcattgat tacattaaat taatgttcca tcactggttt ggttaaaaat tgaacacttg
    16021 aaagtgtaat ttccgtcccc atatactaag catcagtaaa aaactcccgc cttctggcgg
    16081 gagttgctat ttaattacgt tacgccggag ctgacatgaa aaccaaactt aacgaactgc
    16141 ttgaattccc tactcctttt acttacaaag ttatggggca ggcgttacct gagctggttg
    16201 atcaggtggt tgaagtggta cagcgccatg cgccaggtga ctacacccca acggtaaaac
    16261 caagcagcaa aggcaactac cactcggtat ctatcactat caacgccact catatcgagc
    16321 aggttgaaac actgtatgaa gaactgggca aaatcgatat tgtccgcatg gttctgtaac
    16381 tcgcttctcc gttacccggc ccctggtcgg gtaactcccc tctcctcgct gtgatatact
    16441 tttctcccac ttttactcat tctccacgga gatgccgttt tgtatcagga taaaattctt
    16501 gtccgccagc tcggtcttca gccttacgag ccaatctccc aggctatgca tgaattcacc
    16561 gatacccgcg atgatagtac ccttgatgaa atctggctgg tcgagcacta tccggtattc
    16621 acccaagggc aggcaggaaa ggcggagcac attttaatgc ccggtgatat tccggtgatt
    16681 cagagcgatc gcggtgggca ggtgacttat cacgggccgg ggcaacaggt gatgtatgta
    16741 ttgcttaacc tgaaacgccg taaactcggt gtgcgtgaac tggtgacctt gcttgagcaa
    16801 acagtggtga ataccctggc tgaactgggt atagaagcgc atcctcgggc tgacgcgcca
    16861 ggtgtctatg ttggggaaaa gaaaatttgc tcactgggtt tacgtattcg acgcggttgt
    16921 tcattccacg gtctggcatt aaacgtcaat atggatcttt caccattttt acgtattaat
    16981 ccttgtgggt atgccggaat ggaaatggct aaaatatcac aatggaaacc cgaagcgacg
    17041 actaataata ttgcaccacg tttactggaa aatattttag cgctactaaa caatcctgac
    17101 ttcgaatata ttaccgctta atttcatacc tcaatggccc aatttacact gggtcattac
    17161 tcataacacc tgctaatgct ttacatctcc ttcgcgtttg tatattttct ttatcacttg
    17221 cagaaatgcg atgctacgcc ttgctccgta actcacccca ttgattaact tattgaaata
    17281 gttgatcatg atgagcaaaa gcgttccgtt ccaattagac ataagattat tgtaattttt
    17341 ttaaatatta tttttccatg aataaaattg gagtaagtgc gtggatagta ataatcaaat
    17401 tgaatcctgt ttaagcagaa aatcgtcaga aggtaaacca caaatattta caaccttacg
    17461 aaatattgat cttaatcttc tgactatttt tgaagctgta tatgtacata aagggatcgt
    17521 taatgcagcg aaagtgctta atctgacccc ctcggcaatc agtcagtcta ttcagaaact
    17581 gcgcgttata ttccctgacc cattgtttat tcgcaaaggc cagggtgtca ctcctaccgc
    17641 atttgcgatg catctacatg agtatatcag tcagggcctt gagtccattc ttggcgcgct
    17701 ggatatcgaa ggaagctatg ataagcaacg aacgataact attgcgacca ccccctcggt
    17761 cggagctctg gtccttcctg tcatttatcg ggcgattaaa actcactatc cgcagctttt
    17821 actgcgcaac ccgcccatca gcgacgcgga aaaccaactc agtcagtttc aaaccgatct
    17881 catcatagat aacatgtttt gcaccaatcg tacggtgcaa catcatgttc tgttcaccga
    17941 caatatggtg ttaatttgcc gcgagggaaa tccactactc tctttagaag atgacagaga
    18001 gactatcgac aacgctgcac atgtactcct gttaccggaa ggacaaaatt tcagcggtct
    18061 gcggcagaga attcaagaga tgtttccgga ccggcagatc agcttcacca gctacaacat
    18121 tttgacaatc gctgcactgg ttgccaacag tgacatgtta gcgattattc ccagccgttt
    18181 ttataacctg tttagccgtt gctggccgct ggaaaaactg ccttttccgt ccttaaatga
    18241 ggagcaaata gatttctcta tccactacaa caaattcagc ctgcgtgatc ccatcctgca
    18301 cggagtaata gatgttatcc gtaatgcatt ttgaaaaagt attgttacag ccagatcaca
    18361 gcgcagaacg ataaaatggc acaatccaac aacaatttta cattttcgcg accgctttgg
    18421 ctgctttcag gtccgtttca atgatatact gccagtcgtt aattcaaaaa tagttgataa
    18481 ttacaacaat ctattgaatt gaaacgcttt ccttcgtaat tcgcaactgg aacacgcacg
    18541 ctatgagtaa acccattgtg atggaacgcg gtgttaaata ccgcgatgcc gataagatgg
    18601 cccttatccc ggttaaaaac gtggcaacag agcgcgaagc cctgctgcgc aagccggaat
    18661 ggatgaaaat caagcttcca gcggactcta cacgtatcca gggcatcaaa gccgcaatgc
    18721 gcaaaaatgg cctgcattct gtctgcgagg aagcctcctg ccctaacctg gcggaatgct
    18781 tcaaccacgg cacagcaacg tttatgatcc tgggcgctat ttgtacccgc cgttgtccgt
    18841 tctgtgacgt tgcccacggt cgcccggtag ctcctgatgc caatgagcca gtgaaactgg
    18901 cgcagaccat tgccgatatg gcgctgcgtt atgtggttat cacctccgtt gaccgtgatg
    18961 acctgcgcga tggcggtgcc caacactttg cggattgtat tactgccatt cgggaaaaaa
    19021 gcccgcaaat caaaattgaa actctggtgc cggatttccg cggtcgtatg gatcgtgctc
    19081 tggatattct gactgcaacg cccccagatg tgttcaacca taacctggaa aacgtaccgc
    19141 gtatttaccg gcaggtacgg cctggcgcag attacaactg gtcgctgaag ctgctggaac
    19201 gctttaaaga agcgcatccg gaaatcccga ccaagtctgg tctgatggtg ggtctgggtg
    19261 aaaccaatga agaaattatt gaggtaatgc gcgacctgcg ccgtcatggt gtgacgatgt
    19321 taacgctagg gcaatatttg cagccaagcc gccatcacct gccggttcaa cgttacgtta
    19381 gcccggatga gttcgacgaa atgaaagccg aagcgctggc gatgggcttt acccatgctg
    19441 catgcggtcc gtttgtccgc tcttcttacc acgccgattt gcaggcgaaa gggatggaag
    19501 ttaagtaatc ctgtaataat tacttacatc tgtctgataa aaaacccgct ttttgaagcg
    19561 ggttttttgt atcgaacaag atattgaggg agcgtcctgc tcgccacgtc actctttatg
    19621 agagagcttt tcagcctgaa gatcaacgtc tgcgcctttt ttcgccgcag catcgtcatc
    19681 attcatcgcc ttcttgaacc ctttaatggc cgctccaagg tctccgccca gcgtacgtaa
    19741 cttcttagtc ccaaacagca gaacgaccag cgccgcaact accagcagtt tggtaatact
    19801 aatctcaccc atagatacct tcttgacata taaccggcaa cagaacgcgc cttaaaaacc
    19861 aaagaatatt aacggtcatt cgacgcgcgc acacaatagc aatctgtaac aaggtgaatc
    19921 aagcgccgag taaaaaaaca tcataataat tgcggcggcg caaagcgacg gttgtttaag
    19981 acgggcagtt gcgcgcgcac ctgacgcaca cgttcgggcg tcacttccgc cataatgagt
    20041 gcaggcattt ctgacgctgc cgcaatggtg acgccaaagg gatctataat ccggctttgc
    20101 ccgatatttt tgttcccgca ctcccccgcc gccaccatat aacaggtggt atccagcgca
    20161 cgagcggcaa gcaacgttga ccagtggtgc tctttgagcg gcccgcgaac ccaggcggca
    20221 ggaagtacca ggatttcagc tccctgtaat gcctgtgcca gcgccagctc tggaaagcgt
    20281 aagtcataac aggtcatcag accgaccttc atcccctcca cctcgagtaa cggagcaatt
    20341 tcattaccag catcaacacg gcgtgattcc tgaatggcaa atgcatcata gagatgcaat
    20401 ttggcataac gggcgacgat gtttcctgcc tgtagcgcca ccagcatatt ccatgcgcgc
    20461 cccggcgttg aaggaacatg aatcgtcaga attgtcgtca tcatgttacg tttactttct
    20521 cgccgtaaac gtccgaggaa ttcgccttcc agcagctgtg ctgatttaac cgatagatct
    20581 gcatcatgat catcgcgcgc cagcaatgct tccggcagga caaacagcga tacgtcgttt
    20641 tccgccgcct gcgccatcaa cgaggcacaa atctcagcgt tcttttccca cacagatgta
    20701 acagcaaact gtcctgcagc aaccagcatt gccacctcct gtcagagtcg taaaattcgc
    20761 caacgacgat tattcggcgt tacacttatc actcatacaa atcaaatagc aggattttgc
    20821 agtgttacaa cttcttttag cagtttttat tggcggtggt acgggaagcg tggcgagatg
    20881 gctgttaagt atgcgattta acccgctgca tcaggcgatt ccgttgggga cgctggcagc
    20941 aaacctgatt ggggcattca tcataggaat gggattcgcc tggttcagca ggatgacgaa
    21001 cattgatcca gtgtggaaag tattaatcac caccggattt tgtggcggtc taacaacctt
    21061 ctcaacattt tcggcagaag tggtgttttt gttacaagag ggccgctttg gctgggcatt
    21121 actgaacgtt ttcgtcaacc ttctggggtc ttttgccatg accgcactgg cattctggct
    21181 gttttcggcc tcaaccgcac actaaagacc aaaaaaaacc cgctgattaa gcgggttttg
    21241 aattcttgct gacgtatctt acagagcgat tacgtttgca gcagaagggc ctttggcacc
    21301 gttagtgatt tcgaactcta cgcgctgacc ttcagcaaga gttttaaaac cattagtctg
    21361 gattgcagag aagtgtacga acacgtcttt gctgccgtct tccggagtaa tgaaaccgaa
    21421 tcctttggac tcattaaacc acttaacgtt acctttaatc ttagacatca aaattacctt
    21481 tacatgaaaa atagacacaa atgctgtgtc ggttaccagt acaccaattg tggtacgctt
    21541 tgtccagtgc aactttacta aaaagtgata aaaatcgcgt tatttttgat gttacttatt
    21601 ttagttattg attttaaaga agttactaaa acttcatttg tctcaaaact gaaagcgcat
    21661 ccaggcaaag tacacattgc cattgttgta ggtacccgga atgtaggtca tctgaaaagt
    21721 aactgggcca taacccacgg aggccaatgg cagtagaacc gggagaggga tgtaattcca
    21781 gttatcgcgt gccgttacgc cagcggtgaa tcccagacct aaatgaaaat tttcatccgc
    21841 cagcggtcgc caggtacttt cccatccgta tccggcaatc ggttcccatt tgttccacga
    21901 gtccttaaat gccatggcat acaggccatg ccagtttcct ttttcatccc aacgcgacag
    21961 gccaaaaccg ccaccccacg gtcgctcgtt atagcgatcg gttttttctt tgtcgtaagc
    22021 gaaacgtgca tgccaggtga tggcaggaat atataaatca taatgttcag gctgttgcca
    22081 ggtttgtaca atattttctc taaacgttgt catccactca tctgcgttag caaaaacttt
    22141 accaacgctg attaactgaa taaaaacaaa ggaaaagata gcgacatatt tactcacgtt
    22201 catttgtgac cataaaacat ttatcaaaaa tctactacta gcatagcaaa gctacaatta
    22261 acataacctt aacagcccca acataaagaa taatctgaat agctgctcta tttatttccg
    22321 cttgttcaag ttaaatattt ccgcgcatcg atttaaaatg acacatcgtt ttctgcgatg
    22381 ggaatagtca taaaagaaaa agctagttgt ttctgacaac aaaatgcaac caagaaaaaa
    22441 tgacttattt tttaataaga taccaaaaat catcttaaca gggaatatat tatatatcgt
    22501 caacccacgc ccattgcaga ttattttttg agcacacaac cgcagctaac accattgcaa
    22561 ttaacaaatt tgcatcaatc ctccatcaat ttacacacat tattatgtga taattgccaa
    22621 ccgctaaata tgcgttttgt tatctacgta taaaaacagc aacttcaatg tcttaatggc
    22681 agtttttctt gattttaatc agcattcatc gccaatttat tgggcatatt ttttccttaa
    22741 gctttaggaa ttttttatta tttactttgg ggcctggaga caggaaaaat tatgctgaca
    22801 ttcattgaac tccttattgg ggttgtggtt attgtgggtg tagctcgcta catcattaaa
    22861 gggtattccg ccactggtgt gttatttgtc ggtggcctgt tattgctgat tatcagtgcc
    22921 attatggggc acaaagtgtt accgtccagc caggcttcaa caggctacag cgccacggat
    22981 atcgttgaat acgttaaaat attactaatg agccgcggcg gcgacctcgg catgatgatt
    23041 atgatgctgt gtggatttgc cgcttacatg acccatatcg gcgcgaatga tatggtggtc
    23101 aagctggcgt caaaaccatt gcagtatatt aactcccctt acctgctgat gattgccgcc
    23161 tattttgtcg cctgtctaat gtctctggcc gtctcttccg caaccggtct gggtgttttg
    23221 ctgatggcaa ccctatttcc ggtgatggta aacgttggta tcagtcgtgg cgcagctgct
    23281 gccatttgtg cctccccggc ggcgattatt ctcgcaccga cttcagggga tgtggtgctg
    23341 gcggcgcaag cttccgaaat gtcgctgatt gacttcgcct tcaaaacgac gctgcctatc
    23401 tcaattgctg caattatcgg catggcgatc gcccacttct tctggcaacg ttatctggat
    23461 aaaaaagagc acatctctca tgaaatgtta gatgtcagtg aaatcaccac cactgcccct
    23521 gcgttttatg ccattttgcc gttcacgccg atcatcggtg tactgatttt tgacggtaaa
    23581 tggggtccgc aattacacat catcactatt ctggtgattt gtatgctgat tgcctccatt
    23641 ctggagttcc tccgcagctt taatacccag aaagttttct ctggtctgga agtggcttat
    23701 cgcgggatgg cagatgcgtt tgctaacgtg gtgatgctgc tggttgccgc tggggtattc
    23761 gctcaggggc ttagcaccat cggctttatt caaagtctga tttctatcgc tacctcgttt
    23821 ggttcggcga gtatcatcct gatgctggta ttggtgattc tgacaatgct ggcggcagtc
    23881 acgaccggtt caggcaatgc gccgttttat gcgtttgttg agatgatccc gaaactggcg
    23941 cactcttccg gcattaaccc ggcgtatttg actatcccga tgctgcaggc gtcaaacctg
    24001 ggccgtaccc tttcgcccgt ttctggcgta gtcgttgcgg ttgccgggat ggcgaagatc
    24061 tcgccgtttg aagtcgtaaa acgcacctcg gtaccggtgc ttgttggttt ggtgattgtt
    24121 atcgttgcta cagagctgat ggtgccagga acggcagcag cggtcacagg caagtaaata
    24181 gtaatgccgg aggcaagttt cctccggctt tatcatgtca cccactgtgg tatatgcgtt
    24241 gcggtctgcc aactttgccg tgaacaattt cagcaataat cagatggcgg ctggcgcaat
    24301 attcaagata acgcctggca gtggtgcgac tgatggtcag tgcctgcgcc accgtttctg
    24361 ccgtatgttg cacaccaggc tctttaaaca gttttcgcac cgcgtttagc gtcaaggggt
    24421 caatgccggt cggcagttcg tctttgggtt caccgcgagc ataggcatta aacatctcat
    24481 caatttgctt ctggctggcg ctatcaatac tttccagcat atgtttacgc tggcggaaac
    24541 gggttagcgt ttgccccagc cgttcatagg caatgggctt aatgagataa tcaaatacac
    24601 cacaacgtac ggcttcagac accgtttcca tatcgctggc tgcagtggta aacaccacgt
    24661 cgccgggata atgcgcctgc accagttcat gcagtaaatt aatccctcta ccgtcaggaa
    24721 gatagttatc gagcaagatt agccccggct taaaacgctc gatcatcatt cgggcctgcg
    24781 ccaggtttcc cgccagtaat atctgactga atccgggaat gtgacgaata tattcagcat
    24841 gcatctctgc cagcggcgtt tcgtcctcaa cgatcaatag ggttaatgga gctgtcattt
    24901 ggtttcactt tcggaatata gattgaaaat aaggtaccgc agggatcatt atcttcgaga
    24961 gtgataacac caccgcagcg cgttacgtag ctggcaatca agtacaaccc aatgccatgt
    25021 tcaccgggct cgtcagcacg cgtactgacc ccctgctcaa atattttgtc tcgtagagac
    25081 tctggaacgc cgcagccctg atcggcgact tcaatcacca catcatcgcc ttcatcgctg
    25141 aggaataatt caacgatctt gtttccttca tcgctacgca ggctggcttc gaaggcgtta
    25201 tcaagtaaat tgcccacaat ggctgcaaac tcggtgctat ccagtcctgg cggcagttgc
    25261 gaaagctggc taccggggac aatgatcatt tttagcccca gttcccgggc gcgctgcact
    25321 ttaccaaaaa gcagccccgc cacctggcga tcggcaaacg cctcgcgcag gctgtcaata
    25381 agctgttgct gggcctgaga ctccccctgc accatcgcca gcacgcgatc atactctttc
    25441 atctgcaaca gaccattgag cgtcgacatc caattgagat gctcgtgtcg caatgtacga
    25501 aggctctcaa cgtattgttt tatttgcgtc agttgcgcat tgagggtgga aatttcgtct
    25561 ttactgcgaa agctgataat ggcccccagc aaatcatcac ctgaacgaat agcttcccga
    25621 ttggcaataa cgctcagacc gttaaagtta gccaccacat cctgacgttt ttcatcaatc
    25681 tgttcggtaa agaaatcggc gggcctgacc acttcagcaa tgggtttacc caaccattgc
    25741 cgtccgggag agctcagccc cagcatcttt cttgcgttac gattgatggc ggtaatgtaa
    25801 ccatgcggat ccaccgcaat cagcccttca taaaccgaac taaacagcgc ctcttgctga
    25861 cggaccacgc gtgcgatttg ctttggctcc atgcccatca tttgccgacg gatatgcgct
    25921 gccaggaacc acgacagcaa catcagaatc cctaacagca cgacaaacac gcctgccatc
    25981 ggtaataaaa actcagcccg ccagctatcg attttactca ccaggtagcc aatcgacacc
    26041 acgccgatga cttttccatc gtcatcaaag attggcgttt tggcgcgcat cgccatcccc
    26101 attgaccctt tcccggtaat gaagtagctc tcccctttct ccagcgcgcc ctgtttggtg
    26161 aactgcatag gataaccaat tttctccgga ttaggatggt aaaggcggat cgagtgccgg
    26221 tccccaatca ccacataatc aaaatcggta tctctttgta atttgttagc gatggtcgcc
    26281 agccgtttgt agtcacgcgt tttcacctca gagatgacac tgtcattgga ggcaataatt
    26341 ttcgcctgat tcattgccat gtcgcgtaca tgaagcgtta aatagtcctc aaaactggcc
    26401 gtaaaatatt gcgccagggc tgcaatgaca aatattgaga acaccagaat cagcaaaaag
    26461 atacgcagcg gaaatgccag tctttggaaa aatgcaaact gtttattctc gttaagctgc
    26521 aacattgttt tccttgcggg ttgtcccgag cctctttgac ggatgtaatt aaaa
//
LOCUS       DAZFAB010000058        26541 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZFAB010000058 DAZFAB010000000
VERSION     DAZFAB010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26541
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.058"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            137..646
                     /locus_tag="ACHJWA_002774"
     CDS             137..646
                     /locus_tag="ACHJWA_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFO4309334.1"
                     /translation="MNQQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIESTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPYAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            643..2061
                     /gene="phrB"
                     /locus_tag="ACHJWA_002775"
     CDS             643..2061
                     /gene="phrB"
                     /locus_tag="ACHJWA_002775"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFO4309335.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARLLALYIATPRQWAA
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFAASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVQVERTLRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLNEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRQWLPELRNVPGKSVHEPWKWAEKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(2211..3692)
                     /gene="dtpD"
                     /locus_tag="ACHJWA_002776"
     CDS             complement(2211..3692)
                     /gene="dtpD"
                     /locus_tag="ACHJWA_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFO4309336.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELRLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGVVLMIWLYQALKFRNRALALES"
     gene            3963..4706
                     /gene="ybgI"
                     /locus_tag="ACHJWA_002777"
     CDS             3963..4706
                     /gene="ybgI"
                     /locus_tag="ACHJWA_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFO4309337.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            4729..5385
                     /gene="pxpB"
                     /locus_tag="ACHJWA_002778"
     CDS             4729..5385
                     /gene="pxpB"
                     /locus_tag="ACHJWA_002778"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFO4309338.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            5379..6311
                     /gene="pxpC"
                     /locus_tag="ACHJWA_002779"
     CDS             5379..6311
                     /gene="pxpC"
                     /locus_tag="ACHJWA_002779"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFO4309339.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKAKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            6301..7035
                     /gene="pxpA"
                     /locus_tag="ACHJWA_002780"
     CDS             6301..7035
                     /gene="pxpA"
                     /locus_tag="ACHJWA_002780"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFO4309340.1"
                     /translation="MKIDLNADLGEGCTSDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRKNFGRSAMQLPPETVYAQTLYQIGALATITRAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQSGALIEDEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSAFAEKGIVVAA"
     gene            7071..7862
                     /gene="nei"
                     /locus_tag="ACHJWA_002781"
     CDS             7071..7862
                     /gene="nei"
                     /locus_tag="ACHJWA_002781"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFO4309341.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEESQTTRVLRVKLQTADKSIL
                     LYSASDIEMLTPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(7859..8905)
                     /locus_tag="ACHJWA_002782"
     CDS             complement(7859..8905)
                     /locus_tag="ACHJWA_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFO4309342.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLGLHLPAALLLGPMIAGIIFSMRG
                     VTLQLPRSAFLAAQAILGCMIAQNLTGSILTTLEVNWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQHIVWFPPVSINLLLTILLAVVAGTVGCLLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRTA"
     gene            complement(9054..10115)
                     /locus_tag="ACHJWA_002783"
     CDS             complement(9054..10115)
                     /locus_tag="ACHJWA_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4309343.1"
                     /translation="MRFAKGVLLAICLIFLPLKAALALNCYFGTANGAVEKSEAIMPF
                     AVPANSKPGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGIQDPYYQLGVTY
                     EGVDYDASLGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKLCSGDPTVMHKSRTFVA
                     RMRLYVKVRAMPPHDYQSKLSDYIVVQFDGAGSVNEDPTAKNLKYHITGLENIRVLDC
                     SVNFAISPETQVVDFGRFNVLDIRRHTMSQQFKITTTKSQNDQCTDGFKVSSSFYTDE
                     TLIDEDKSLLIGNGLKLRLLDENASPYTFNKYSEYADFTSDLLVYEKTYTAELSSTPG
                     TPIDVGPFDTVVLFKINYN"
     gene            complement(10112..10843)
                     /locus_tag="ACHJWA_002784"
     CDS             complement(10112..10843)
                     /locus_tag="ACHJWA_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO4309344.1"
                     /translation="MTFVKGFPLILLVASMCSHGAVQPDRTRIIFNSKDKATSLRVEN
                     RSDKLPYLAYSWIENEKGEKSDEFLVALPPIQRLEPKATSQVRIMKQAATAKLPTDRE
                     SLFYYNLREIPPVPEGSEGHAILQVAVQSRIKLFWRPAALRKKMGDHVEQQLQVSQQN
                     NQLTLKTPTGYYLTIAYLGRDEKGVLPGFKSTMVAPFSSVTTSTGSYSGKQFYLGYMD
                     DYGALRMNTLSCQRQCRLQPVENKK"
     gene            complement(10858..13317)
                     /locus_tag="ACHJWA_002785"
     CDS             complement(10858..13317)
                     /locus_tag="ACHJWA_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO4309345.1"
                     /translation="MDAVNIYRLSVLSCLAMVTPPALTAEFNLNVLDKSIRDSVDISL
                     LNQKGVVAPGDYFVSVTVNNNKISNGQQIRWQKSGDKIIPCINESLIELFGLKSDFRK
                     KLPAIKECVDFSVFPEIIFTFDQANQQLNITIPQAWLAWHSENWTPPSTWNNGIPGFL
                     MDYNLFASTYRPQSGSSSNNLNAYGTTGLNAGAWRLRSDYQLSQSDSGDNREQSGAIS
                     RTYLFRPLPQIGSRLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISHSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVSEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGRPRSSMSHHTEDETFISHEVSWGMLSNTSLYGGMLLAGDD
                     YRSGALGIGQNMLWMGALSFDVTWADSHFDTQQDEQGYSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYIDHKYNDADAQDEKQTISLSFGQPITLLNLNLYANILHQSWWN
                     ADTSTTANITVGFNVDIGDWKDISVSTSFNTTHYEDKDRDNQIYFSISLPIGESGRLG
                     YDMQNNSNTTTHRMSWNDTLDERNSWGMSAGIQSDRPDNGAQVSGNYQHLSSAGEWDL
                     TGTYAANDYTSASASWSGSFTATQHGAAFHRRSSTNEPRLMVSTDGVGDIPIQGNIDY
                     TNRFGIAVVPFVSSYQPTTVAVNMNDLPDGVTVSENVVKETWTEGAIGFKSLASRAGK
                     DLNVIISDANGHFPPLGADVRQAEGGVSVGMVGENGHAWLSGVDENQQFTVHWGDQKT
                     CAIHLPEHLEDVTKRLILPCH"
     gene            complement(13367..13933)
                     /locus_tag="ACHJWA_002786"
     CDS             complement(13367..13933)
                     /locus_tag="ACHJWA_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4309346.1"
                     /translation="MFKGQKTLAALAISLLFASPVFAADEGSGEIHFKGEVIEAPCEI
                     HPDDLDMNIDLGEVTTSHINREHHSEKKPVNIRLINCDIAGWDDGKGGVVSKVGVTFD
                     STAKTTGATPLLSNISAGEATGVGVRLMNKDESFVTLGTEAPTIDLLASSTEQTLNFF
                     AWMEQMDNAVSVTAGAVTANATYVLDYQ"
     gene            complement(14320..15603)
                     /gene="gltA"
                     /locus_tag="ACHJWA_002787"
     CDS             complement(14320..15603)
                     /gene="gltA"
                     /locus_tag="ACHJWA_002787"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFO4309347.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDVKH"
     gene            complement(15809..15916)
                     /locus_tag="ACHJWA_002788"
     CDS             complement(15809..15916)
                     /locus_tag="ACHJWA_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4309348.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            16297..16701
                     /gene="sdhC"
                     /locus_tag="ACHJWA_002789"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             16297..16701
                     /gene="sdhC"
                     /locus_tag="ACHJWA_002789"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO4309349.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            16695..17042
                     /gene="sdhD"
                     /locus_tag="ACHJWA_002790"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             16695..17042
                     /gene="sdhD"
                     /locus_tag="ACHJWA_002790"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFO4309350.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFCASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            17042..18808
                     /gene="sdhA"
                     /locus_tag="ACHJWA_002791"
     CDS             17042..18808
                     /gene="sdhA"
                     /locus_tag="ACHJWA_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFO4309351.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            18824..19540
                     /gene="sdhB"
                     /locus_tag="ACHJWA_002792"
     CDS             18824..19540
                     /gene="sdhB"
                     /locus_tag="ACHJWA_002792"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFO4309352.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            19841..22642
                     /gene="sucA"
                     /locus_tag="ACHJWA_002793"
     CDS             19841..22642
                     /gene="sucA"
                     /locus_tag="ACHJWA_002793"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFO4309353.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            22657..23874
                     /gene="odhB"
                     /locus_tag="ACHJWA_002794"
     CDS             22657..23874
                     /gene="odhB"
                     /locus_tag="ACHJWA_002794"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFO4309354.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            23968..25134
                     /gene="sucC"
                     /locus_tag="ACHJWA_002795"
     CDS             23968..25134
                     /gene="sucC"
                     /locus_tag="ACHJWA_002795"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFO4309355.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            25134..26003
                     /gene="sucD"
                     /locus_tag="ACHJWA_002796"
     CDS             25134..26003
                     /gene="sucD"
                     /locus_tag="ACHJWA_002796"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFO4309356.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
BASE COUNT         6441 a         6611 c         7214 g         6275 t
ORIGIN      
        1 attcgctgct ttgatgatga tgtaaaatga ttgtttttga actggatata acagcttggt
       61 agggaagaaa gcataacccc acaaaattca tttaccatcg tctacactta ctcctgtaaa
      121 ccgttcagga gcagtaatga atcagcaacg atttgatgac agcactctaa tccgtatctt
      181 cgcgcttcat gagttacatc gactgaaaga acatggctta acgcgcgggg cgcttctcga
      241 ttatcacagc cgctataaac tcgtatttct ggctcattcc cagccggaat accgcaaact
      301 tggcccgttc gtggctgata ttcaccagtg gcaaaatctg gatgattttt acaaccagta
      361 ccgccaacgc gtaattgttt tgctttctca tcccgccaac ccgcgcgatc acaccaacgt
      421 tttgatgcac gttcagggtt attttcgccc gcatattgag tccacagaac gccagcagct
      481 ggctgcgctt atcgacagtt atcgccgtgg cgagcaaccg cttcttgcgc cgctgatgcg
      541 tatcaaacac tatatggcgc tttatcctta cgcctggctt tcagggcagc gttatttcga
      601 actttggccg cgtgtgatta acttgcgcca ttcaggagtt ttatgactac ccatctggtc
      661 tggtttcgcc aggatttacg tctgcatgat aatctcgcgc tggctgccgc ctgccgcaat
      721 tcatctgcac gcctgctggc gttgtatatc gctacaccac gccagtgggc ggcgcataat
      781 atgtcgccgc gtcaggctga acttatcaat gctcaactga acgggctaca aatcgcgctg
      841 gcggaaaaag gcattccttt attgttccgt gaagtggatg actttgccgc cagcgtcgaa
      901 atagttaaac aggtgtgcgc ggaaaacagc gttactcatc ttttttataa ctatcagtat
      961 gaggtgaatg agcgggcgcg ggatgtgcag gttgagagaa ctctgcgtaa cgtggtgtgt
     1021 gaaggatttg atgacagcgt gatccttccg cctggcgcgg tgatgaccgg caatcatgag
     1081 atgtacaaag tctttacgcc ttttaagaat gcctggctga aacggctgcg ggaagggatg
     1141 ccggagtgcg tcgctgcacc aaaagttcgt agtagcggat cgataaagcc cgcgccatcc
     1201 attacgctga attatcctcg tcagtctttc gatactgcgc atttcccggt ggaagaaaaa
     1261 gcggcgattg cgcaattacg ccagttttgc cagaacggtg ccggagaata tgagcaacaa
     1321 cgagattttc cggcagtgga aggcaccagt cgtttgtccg ccagcctggc aacgggcggg
     1381 ttatcgcctc gccagtgttt gcatcgcttg ttggcggaac agccgcaggc gctggacggt
     1441 ggggccggta gtgtctggct taatgagctg atctggcgcg agttctaccg tcatctgatg
     1501 acgtattacc cctcgttgtg taaacattgt ccgtttattg cctggacgga tcgtgtgcag
     1561 tggcagagca atcccgcaca tttacaggcc tggcaggaag gcaaaacggg atacccgatt
     1621 gtcgatgctg ccatgcgtca gcttaacagc actggctgga tgcataacag gctacggatg
     1681 attacagcca gttttctggt gaaagatttg ttgatcgact ggcgcgaagg cgagcgatat
     1741 ttcatgtcgc agctgattga tggtgatttg gcagccaata acggtggctg gcagtgggcg
     1801 gcttctacgg gtactgatgc agcgccgtat tttcgtattt tcaacccgac aacccagggc
     1861 gagaaatttg accgcgaagg cgagtttatc cgccagtggc taccggaact gcgcaatgta
     1921 ccggggaaat cggtgcatga gccgtggaag tgggctgaga aagcaggtgt gaagctggat
     1981 tatccgcaac cgatagtcga gcacaaagag gcgagagtac aaacgttggc agcgtatgag
     2041 gcggcgcgga aggggaaata acagacgcgt caggcaatcg agcccagatg ccggatgcgg
     2101 cgtgaacgcc ttatccagcc tacaaatttg tgcaaattca ataaattgca ggaaatacgt
     2161 aggcctgata agacgcgtca agcgtcgcat caggcatcgg tgctggccta ttaagactcc
     2221 agcgccagcg cgcggtttct gaatttcagc gcctgataca gccaaatcat cagtaccact
     2281 cctacacacg ccagtgcgcc ccaggtaatt tgatcaaata cttcaatata tgcattgatg
     2341 gagtagttga tcgccccgga agcatcaaac gaagcctgcg atgtctgatc ggcaatcacg
     2401 cccgccagat agttcgcaat cgcgccggaa agcagcatat aaatgccggt taatacgccg
     2461 gtcacaccgg ggatttcaat gcgcgtaatc tgcgacatag caaccgggtc gataaacagt
     2521 tccgcaaagc ccatcaccgc caggcctaat accatcagtg gcagagaaga gtgaccatac
     2581 attgcggacc agcgggcgct taaggtcaga atgcagaatc cggcgctcat caggccaagg
     2641 ccaagagcaa attttcccca gatgcgcacg gtacgattac ccgcgacact ctcttttacc
     2701 acccacgcca ggaacacacc gcacagcata actgcgaagg cattaatcga ctggaacatc
     2761 gcggtcggaa cggtataacc aaacatatcg cgattaacga agcggtcgat ataaaggcta
     2821 atcgagctac cgccctgttg tgcgaaggcc cagaacaaca tactgaagaa ggtgagcgtc
     2881 acaatcagcc tcagctcctt ccgctgtttc tggttttctg ctttgcgata aatttttgcc
     2941 agtacaccca ggccaatgat agtcgcgaca atcaaggcgt ataccgacca ttctttccag
     3001 aacagtacgg taatcagcgc aggcgttgcc accagcagaa ccagcagcca tccccagttc
     3061 ggcagcagaa agtttgtcgc acgcagtact tttttgttaa cgccgcgggt atgtgtgaaa
     3121 tgacgattgc cacataagaa aataaccaga cccgcgatca tacccaccgc cgccaggcca
     3181 aagcccatcg cccaactgta ctcttcctgg gcgtagccac aggcaatagg tgcgataata
     3241 gaccccacgt tacccgctgc atacatcagc gagaaaccgc catcacgacg cggatcggtt
     3301 ggctcataca gctcgccgag caggcaactg acattggatt taaacagacc atagccacaa
     3361 acgattatcg ccagggacag atagaggaat gacggatgga tctcacttgc acccaacacc
     3421 acatgaccga tcgccatcaa taacgccccc agcatcaccg ccatgcgatt gccgagaact
     3481 ttatccgcca aaaagccacc gaggattggc gtgacataca ccagcgaaca gtaggcgcta
     3541 aataactcgt aggcgtgagt atcgttgtat tttagttgat tggtgagata gagaatcagc
     3601 agggcacgca tgccgtaaaa gctaaaatat tcccagattt gcagcgcaac gacatagtaa
     3661 atagcgcgcg gttgtgatgc gtgtttattc ataataattc cacaattgac ggcaacgtca
     3721 cgtaatagtt gcgcggcgtg cttgtgtttc cttaaagtta aaactttgat acagatctga
     3781 ttatttatgc aatatgctgt ctgattgcat aaatatacat tagctgaagc gtgatgatat
     3841 aaacaatttg cgtcttttgg cgtcgggttt gtttcttaat atgtcaaaag attggctatc
     3901 gacgaacagg cgaggtaacg ctatgttaac ggtgtagcta tcagaatacg gaaggcgcga
     3961 agatgaaaaa caccgaactg gaacaactga ttaacgaaaa actgaacagc gcggcgatta
     4021 gtgattacgc gccgaatggt ttgcaggtgg aaggcaaaga gacggtgcaa aaaattgtta
     4081 ccggtgtcac tgccagccag gctttgctcg atgaggcagt gcgtctgggc gcggatgcgg
     4141 tcattgtgca tcatggctac ttctggaaag gcgaatctcc ggtcattcgc ggcatgaagc
     4201 gtaaccgttt aaaaacgttg ctggcgaatg atatcaacct gtatggctgg catttgccgc
     4261 ttgacgcaca tcctgagctg ggcaataacg cacagctggc ggcgttactg gggatcacgg
     4321 tcatgggcga aattgagccg ttggtgccgt ggggcgaact gaccatgcct gtgccgggac
     4381 tggagctggc ttcctggatt gaagcgcgtc tgggacgtaa gccgttatgg tgtggcgata
     4441 ccggacctga ggtggtccag cgcgtcgcct ggtgcacggg cggcgggcaa agttttatcg
     4501 atagcgccgc gcgttttggc gtggatgctt ttattactgg cgaagtttct gaacagacca
     4561 ttcattcagc ccgcgagcag ggattgcatt tttatgctgc aggtcaccat gccactgaac
     4621 gtggtggtat tcgcgcattg agcgagtggc tgaatgaaaa taccgatctt gatgtgacct
     4681 ttattgatat tcctaatcct gcataacgaa taatcagagg gatcgaaagt gcaacgagcg
     4741 cgttgttatc tgataggtga aacggcggta gtgctggaac tggaaccgcc ggtgacgctg
     4801 gctagccaga aacggatctg gcgactggcg cagcgtctgg tggatatgcc gaatgtggtt
     4861 gaagccattc ccggcatgaa caatatcacg gtgattttgc gtaatcctga gtcgctggcg
     4921 ctggatgcca tagagcgttt gcaacgctgg tgggaggaga gcgaggccct ggaacctgag
     4981 tctcgcttta ttgaaattcc ggtggtttac ggtggtgcag gcggaccgga tttggcggtg
     5041 gtcgcggcgc attgcgggtt gagcgaaaaa caggttgttg aattgcactc ctccgtggaa
     5101 tacgtggtct ggtttttagg ttttcaaccg ggcttcccgt atctcgggag tttgccggaa
     5161 caactacaca cgccacggcg cgctgaaccg cgcttgctcg ttccggcagg ttctgtcggg
     5221 atcggcgggc cgcagactgg tgtttatccg ctggcaacgc cgggtggctg gcagttgatt
     5281 ggtcatacct cactcagcct gtttgatccg gcgcgtgacg aacccatctt attacgtccg
     5341 ggagacagcg tgcgctttgt accgcaaaag gagggagtat gctgaagatt attcgtgcgg
     5401 gcatgtatac cactgtgcaa gatggcggtc gtcacggttt tcgccagtcg ggtatcagcc
     5461 actgcggcgc actggatatg cctgcgttac gcattgctaa cctgctggtg ggtaatgatg
     5521 ccaatgcccc cgcgctggag atcacgctcg gtcagttaac ggttgagttc gaaactgatg
     5581 ggtggtttgc tctgacgggt gccggttgcg aagcgcggct ggatgataat gccgtctgga
     5641 ccggctggcg attgccgatg aaagcaggcc agcgtttaac gcttaaacgc ccgcagcacg
     5701 ggatgcgcag ttatctggcg gtcgcgggtg gtattgatgt tccgccggta atggggtcat
     5761 gcagcaccga tctcaaagtg gggattggcg ggctggaagg ccgtttactg aaggatggtg
     5821 accgactccc gattggcaaa gcgaagcgtg attttatgga agcgcagggc gttaaacagc
     5881 tgctgtgggg caaccgcatt cgcgccttgc cggggccgga atatcatgag ttcgatcgcg
     5941 cctcgcagga tgcattctgg cgttcgccct ggcagcttag ctcgcaaagt aaccgcatgg
     6001 gctatcgctt acaggggcaa attttaaaac gcaccaccga tcgcgaactg ttatctcacg
     6061 gtttgttacc gggcgtggtg caggtgccgc ataacgggca gcccattgtg ttgatgaacg
     6121 acgcacagac caccggtggt tacccgcgta ttgcctgtat cattgaggct gatatgtacc
     6181 atctggcgca aattccgctc ggtcagccga ttcattttgt ccagtgttca ctggaagagg
     6241 cactgaaagc gcggcaagat cagcaacgtt atttcgaaca attagcgtgg cggctgcaca
     6301 atgaaaattg acctgaacgc cgatctgggc gaaggctgca ccagcgacgc agagctatta
     6361 acgctggttt cctctgccaa tattgcctgt ggatttcatg caggcgatgc gcaaaccatg
     6421 caggcttgcg tgcgtgaagc aataaaaaat ggtgtcgcga ttggcgctca tccgagtttt
     6481 cccgacagga aaaattttgg tcgcagcgct atgcagctgc cgccagaaac cgtttacgcc
     6541 cagacgctgt atcaaattgg cgcgctggca acgattaccc gtgcgcaagg tggtgtaatg
     6601 cgtcatgtca aaccgcacgg catgttatac aaccaggcgg cgaaagaggc acaactggca
     6661 gacgccatcg ccagagcggt atacgcttgc gatccagcat tggttctcgt tgggctggca
     6721 ggaagcgagc tgattcgtgc aggcaagcaa tatggcctga caacgcgcga ggaagtgttt
     6781 gccgatcgcg gttatcaggc tgacggctcg ctggtgccgc gaagccagtc aggcgcgctg
     6841 attgaagacg aagaacaggc gcttgcgcaa acgttggaga tggtgcaaca cggcagagtc
     6901 aaaagtatca ccggcgaatg ggcaacggtc acggcgcaaa cggtctgcct gcatggcgac
     6961 ggcgagcacg cactggcgtt cgcccgccga ctacgctctg catttgccga aaaggggatt
     7021 gttgtcgcag cataaccccg attaataaag aatgaaaaaa ggatatcacc atgcctgaag
     7081 gcccggagat ccgccgtgca gcggataacc tggaggcggc gatcaaaggc aaaccactaa
     7141 ctgatgtctg gtttgccttc ccgcagttaa aaacttatca atcacaactt atcggtcaac
     7201 acgttaccca tgtggaaacg cgtggtaagg cgttgttaac tcatttttcc aacgacttaa
     7261 cgctctacag ccataatcag ctttatggcg tctggcgcgt ggttgatacc ggcgaagagt
     7321 cgcagaccac gcgagtattg cgggtaaaac tgcaaacggc tgacaaaagc attctgcttt
     7381 atagcgcctc ggatattgag atgttgaccc cggaacaact gaccacgcat ccgtttttac
     7441 aacgcgttgg tcccgatgtg ctggatccga atctgacgcc ggaggtggtg aaagaacggt
     7501 tattgtcgcc gcgctttcgt aaccgtcagt ttgctggatt actgctcgat caggcgtttc
     7561 tggctgggct tggcaattat ttgcgtgtgg agatcctctg gcaggttggg ttgactggaa
     7621 atcataaagc gaaagacctc aatgcggcgc aactggatgc actcgcacac gcgttactgg
     7681 atattcctcg gttttcttac gctacgcggg ggcaggtgga tgagaataag catcatgggg
     7741 cgctgtttcg ctttaaggtt tttcatcgtg atggcgaact gtgcgaacgc tgtggcggca
     7801 tcattgagaa aaccacgctg tcatctcgcc cgttttactg gtgtccgggc tgccagcact
     7861 aggccgtacg tttcggcgca taggttgaaa taaaccgcgc aatggcaggc cctgtcagca
     7921 aaatactgaa caggcgcagg gtttgcatcg ccataatgag cgccatatcg gcattgctcc
     7981 ctgcggcgat gaccgccacg gtatcaagcc cgccggggct ggtggcgagg taggcagtca
     8041 taaaatcaat gtgcataaac cgggtcagtc cccacgccat acccgcacaa atagccagca
     8101 gagcaaaaat cgacagcagg atttgtggta gcgggcgtag tgcccgcagt aagatttgtt
     8161 tatcgaaacc aaggccaatc cgccagccaa ttgccatata cgccatcgcc agtagccatt
     8221 ccggtagttc gatggtgatg agctgaccag actggagcac tgcgcccgcc agcatcggga
     8281 tgagcatcgt gccggaagga agacgcagca ggcatcccac tgtaccggca acgaccgcga
     8341 gcaaaatagt aagcaggaga ttaatgctca ctggcgggaa ccagacgata tgctggttaa
     8401 ccgcttcagc gttatcgccc agcatcattc gcgtgaccag aactgcggcc cccgcgacaa
     8461 acagcactcg cagatactgc ataaacgcca ccaggcggat atctgcgccg taatcctgcg
     8521 ccattgcgac cattgccgcc gccccgccag gcgaggagcc ccaggcacca gtatttccgg
     8581 gcagtgagct atagcgcacc agtagccatc ccacgatagc gctggaaagc aacgtcacca
     8641 gtaaaatagc taacacaatc ggccagttaa cctccagcgt ggtcaggatc gaaccggtca
     8701 agttttgcgc aatcatgcag ccaagaatgg cctgcgcggc gagaaaagcg gagcggggga
     8761 gttgcagggt aaccccgcgc atactgaaga tgatcccggc gatcatcggt ccaagtaaga
     8821 gtgccgccgg gagatgtaat ccgaggaaac caatagaaag gagaagtgat agcacacata
     8881 acattcccca ctgcaaaact ggcatccttt gctccctcat tattttctct ttggtgtatc
     8941 gatacagcat aaagaatgcc gggcatggaa aaaataacgg taatcaataa aagccggaaa
     9001 attggcaatt aaccgaagaa gccctctccg gctggaaagg gcaataggct aaatcagttg
     9061 tagttaattt tgaaaagtac cactgtatca aagggaccaa cgtcgatggg cgtacccggc
     9121 gtggatgaca attctgccgt gtaggttttt tcatacacta acaggtcgct ggtaaaatcg
     9181 gcatactcac tgtatttgtt gaaagtatag ggtgaggcat tttcatccaa caatcgaagc
     9241 ttcaagccat tgccaatgag cagtgattta tcttcgtcga taagggtttc atcggtataa
     9301 aacgaagagc tgactttaaa accgtcggta cactggtcgt tttgtgattt ggttgtagtt
     9361 attttaaatt gctgcgacat cgtatggcga cgaatatcca gcacgttaaa tcggccaaaa
     9421 tcaacgacct gggtttcagg tgagatagca aagttgacgc tgcaatccag cacgcggata
     9481 ttttccagac cggtaatgtg atatttcaga ttcttcgcgg tagggtcttc gttcacgctg
     9541 cctgcgccat caaactgtac gacgatgtaa tcgctgagtt tgctctgata atcatggggc
     9601 ggcatggcgc gaactttaac gtaaagccgc atacgcgcca caaacgtccg cgatttgtgc
     9661 ataacggttg gatcgccgga gcaaagttta ttttgccagc ccatcgcaat gatctgctcc
     9721 ggggtgtaaa tatcaatatt cttactgtcg atgcactgat tcgtgtcgat gcggcttttc
     9781 ccaaggcttg cgtcatagtc cacgccttca taggttacgc ccaattgata atagggatcc
     9841 tgaataccgg ggtaagggtt tacccaggcg tagacgtgct cgctttcaaa attaccgttt
     9901 gtgttgttgt cacagtagac cggaattttt atatcgtcag attcccagat tttatcgccg
     9961 ggtttggaat tggccggaac ggcgaagggc ataatagctt ccgatttttc tacagcgccg
    10021 tttgcggtac caaaatagca gttcagcgcc agcgccgctt ttagcggcaa aaaaatcaga
    10081 cagatggcga gcaacacgcc tttggcgaat ctcatttttt attctccacc ggctgcaggc
    10141 ggcattgccg ctggcaggag agcgtgttca tacgcaatgc gccgtagtcg tccatgtagc
    10201 caagataaaa ctgcttgccg ctatagctgc ccgtgctggt ggtgaccgaa ctgaacggtg
    10261 cgaccatcgt gcttttaaag ccaggcagca cgcctttctc atcccgccca agataggcaa
    10321 tagtcagata gtatccggtc ggggttttca gggtaagctg gttattctgc tggctaacct
    10381 gtagctgttg ctcaacatga tcgcccattt ttttgcgtaa tgcggcgggt cgccagaata
    10441 gcttaatgcg actttggacg gcgacttgca ggatcgcatg gccctcgctg ccttccggga
    10501 caggcggaat ttcacgcagg ttatagtaaa agagagattc gcgatcggtg ggtaatttcg
    10561 cggtggcggc ttgtttcatg atccgaacct gcgaagtcgc ttttggctcc agtcgctgga
    10621 taggcggcag ggcgacgaga aattcatcgc ttttctctcc cttttcattt tctatccatg
    10681 aataggcgag gtaggggagt ttatcgctgc ggttttccac gcgcaggctg gttgctttat
    10741 ctttgctatt aaagatgatg cgcgtgcggt caggctgcac tgcgccgtgg ctgcacatac
    10801 tggcaaccag aagaatcaaa gggaatcctt taacaaatgt catcgtcttt tccttaatta
    10861 atgacagggt aaaatcaggc gcttagtcac atcttccaga tgttccggca gatgaatggc
    10921 acacgttttc tggtcgcccc agtgcacggt aaattgttga ttttcatcga ccccactcag
    10981 ccatgcgtga ccgttttcgc caaccatccc gacgctgaca ccgccttcag cctggcgaac
    11041 atcggcaccg agcggcggga aatgcccgtt agcatcgctg ataatgacgt tgagatcttt
    11101 cccggcgcgg gacgccagcg atttaaagcc gatggcgcct tcggtccagg tttctttcac
    11161 cacgttttcg gaaacggtaa cgccgtcagg caagtcgttc atattgaccg ccaccgtcgt
    11221 aggctggtag ctggaaacaa acggtaccac ggcaatacca aagcggttgg tgtagtcaat
    11281 attgccctga atcggaatat cgcccacgcc gtcggtgctg accatcaggc gtggttcatt
    11341 ggtggaactg cggcgatgga atgccgcgcc atgttgagtc gcggtaaacg agccgctcca
    11401 actggcactg gcggaagtgt aatcattggc ggcataggtt ccggtcagat cccattcccc
    11461 cgccgaactg aggtgttggt aattgccgct gacctgcgca ccgttatccg ggcgatcgga
    11521 ttgtattcca gccgacattc cccaactgtt tcgttcatcc agggtatcgt tccacgacat
    11581 gcggtgggtg gtagtattac tgttgttctg catgtcataa cccagtcgac cactttcccc
    11641 aatcggtaag gagatcgaaa agtaaatctg gttgtcgcga tctttatctt cataatgggt
    11701 ggtgttgaac gaggtcgaga cagaaatatc tttccagtcg ccaatgtcga cgttaaaacc
    11761 gacggtaatg ttggccgtgg tggaggtatc cgcattccac cagctctgat gcaggatgtt
    11821 ggcgtagaga ttaagattca gcagagtaat aggctgaccg aaggagaggc tgattgtttg
    11881 cttttcatcc tgcgcgtcgg cgtcattgta tttgtgatca atgtagttag cgtagctgtg
    11941 aaaatggcga tcggagaagc gataggctgc caatgagatg gtgctgttgg tcgcatcaac
    12001 ctgtttgctg tagttaaagc ggtagctgta accctgctca tcctgttggg tatcaaaatg
    12061 gctgtcggcc caggtcacgt caaacgacag cgcccccatc cagagcatat tttgcccaat
    12121 acccagcgcg ccggaacgat agtcatcgcc tgcgagaagc atgccgccat atagtgaagt
    12181 gttggagagc attccccagg agacttcatg gctaatgaag gtttcatcct cggtgtggtg
    12241 cgacatagag gaacgcgggc gaccggcagc cagtttatag cgcacctggc cctgacgcgt
    12301 caggaacggg gtagaggccg ctgaaacctg gaagttattc acccgtccat cttcttcgct
    12361 cactttgaca tccagtgtac cttgcactga ctgattcagg tcatcaataa taaatgggcc
    12421 tggtggaact tttttctggt aaatcacgcg accagaatgg cttatcgtga ccgtggcgtt
    12481 agtctgtgcg atgccgctga tttgcggcgc gtagccgcgt aactcccagg ggagcattcg
    12541 atcgtcgctg gctaacgccg caccggtata ggagaaacca tcaaaaatgt tagagctaaa
    12601 atcggtttcg cccagggtta atcgggaacc tatttgcggt aacgggcgga acaggtaggt
    12661 ccgtgaaatc gccccagatt gttcacggtt gtcgccgcta tcggactggc taagctgata
    12721 atcgctgcgc agacgccatg ccccggcgtt gaggcccgtg gtgccatagg cgttcaggtt
    12781 gttgctgctg ctgccgcttt gtgggcgata agtgctggca aacaggttgt aatccatcag
    12841 gaagccagga ataccgttat tccacgttga gggtggcgtc cagttttctg aatgccacgc
    12901 cagccatgcc tgcgggattg taatattgag ctgttgattt gcctgatcga aagtaaagat
    12961 gatttcaggg aagacactaa aatcgacaca ttcttttatt gccggtaatt ttttacgaaa
    13021 gtcagattta agtccgaaga gttcgatcag cgattcattg atacaaggaa taattttatc
    13081 gccagatttt tgccagcgaa tttgttgccc attactgatt ttattattat taaccgtaac
    13141 actaacaaag taatcgccgg gagctacgac gcctttttga ttgagcaacg aaatatcaac
    13201 gctatcccga atcgacttat cgagaacgtt aaggttaaat tcagctgtca gcgcaggagg
    13261 ggttaccatt gccagacagg aaagaacaga gagtcgataa atattcacgg catccatacc
    13321 tgaacaatat tcgcaaaagg cggctaatca gccgccttaa aaaggattat tggtaatcca
    13381 gaacgtaagt tgcgttagca gtaacggccc ccgcggtaac cgaaactgcg ttatccatct
    13441 gttccatcca ggcaaagaag tttaacgttt gttctgttga acttgccaat aagtcaattg
    13501 ttggcgcttc tgttcccaat gtaacaaatg attcatcttt gttcatgaga cgtacgccaa
    13561 cgccggtagc ctcgccagca ctgatgttac taaggagcgg ggttgcacct gtcgttttag
    13621 ccgtgctgtc gaaggtcacg cctacctttg agaccacgcc acccttgcca tcatcccagc
    13681 ctgcaatatc acagttgatt aagcgaatat tgacaggttt tttctcgcta tgatgttctc
    13741 gatttatgtg ggatgtggtt acttcaccca ggtcaatgtt catatcaagg tcatcagggt
    13801 gaatttcaca tggtgcttca ataacttcac ctttaaagtg gatttcacca gatccttcat
    13861 ctgcagcaaa aacaggtgaa gcgaatagta gagatatggc cagggcggcc agtgtttttt
    13921 gtcctttaaa catacatgaa tccttcatgg gctatgataa agtatactaa ttattccgac
    13981 agaatagtgg ttttttgttc atgtaataat attaatgttg ctacattatg tttgagagtt
    14041 tctcaaagct ctcgaatcat aaataaagtg aattaatcct attacgagtt tatttaattg
    14101 tgggtgctac ccaatgtatt ctacatcgat tggcgggata acggtggtgg tgaatgaaaa
    14161 attaggattt tatttagtta tcggatatgt attcttgtaa cgtgtaatta tgtatttgtg
    14221 tccgattacg tttttttgtc gatagacata actaacgaac aaaaaaaagc ccgccagata
    14281 ttcaggcggg ctaaacgata tatggttcaa atcagataat taatgtttaa catctgattt
    14341 aaagtctcgt ttttcatagc cggtgtacaa ctggcgcgga cgggcgatct tcatgccgtc
    14401 actgtgcatt tcgctccagt gagcgatcca gccaacggta cgggccatag cgaagatgac
    14461 ggtgaacatg gaagacggaa tacccatcgc tttcaggatg ataccagagt agaaatcgac
    14521 gttcgggtac agtttcttct cgataaagta cgggtcgttc agcgcgatgt tttccagctc
    14581 catagccact tccagcaggt catctttggt gcccagctct ttcagaactt catggcaggt
    14641 ttcacgcatt acggtggcgc gcgggtcgta atttttgtac acgcggtgac cgaagcccat
    14701 caggcggaaa gaatcatttt tatctttcgc acgacgaaca aattccggaa tgtgtttaac
    14761 ggagctaatt tcttccagca ttttcagcgc cgcttcgtta gcaccaccgt gtgcaggccc
    14821 ccacagtgaa gcaatacctg ctgcgataca ggcaaacggg ttcgcacccg aagagccagc
    14881 ggtacgcacg gtggaggtag aggcgttctg ttcatggtca gcgtgcagga tcagaatacg
    14941 gtccatagca cgttccagaa tcggattaac ttcatacggt tcgcacggcg tggagaacat
    15001 catattcagg aagttaccgg cataggagag atcgttgcgc ggataaacaa atggctgacc
    15061 aatggaatac ttgtaacaca tcgctgccat ggtcggcatt ttcgacagca ggcggaacgc
    15121 ggcaatttca cgatgacgag gattgttaac atccagcgag tcgtgataga acgccgccag
    15181 cgcgccggta ataccacaca tgactgccat tggatgtgag tcgcgacgga aagcgtggaa
    15241 cagacgggta atctgctcgt ggatcatggt atgacgggtc accgtagttt taaattcgtc
    15301 atactgttcc tgagtcggtt tttcaccatt cagcaggatg taacaaactt ccaggtagtt
    15361 agaatcggtc gccagctgat cgatcgggaa accgcggtgc agcaaaatac cttcatcacc
    15421 atcaataaaa gtaattttag attcgcagga tgcggttgaa gtgaagccag ggtcaaaggt
    15481 gaacacacct tttgaaccga gagtacggat atcaataaca tcttgaccca gcgtgccttt
    15541 cagcacatcc agttcaacag ctgtgtcccc gttgagggtg agttttgctt ttgtatcagc
    15601 catttaaggt ctccttagcg ccttattgcg taagactgcc ggaacttaaa tttgccttcg
    15661 cacatcaacc tggctttacc cgtttgttat ttggctcgcc gctctgtgaa agaggggaaa
    15721 acctgggtac agagctctgg gcgcttgcag gtaaaggatc cattgatgac gaataaatgg
    15781 cgaatcaagt acttagcaat ccgaattatt aaacttgtct accactaata actgtcccga
    15841 atgaattggt caatacttcc acactgttac ataagttaat cttaggtgaa ataccgactt
    15901 cataactttt acgcattata tgcttttcct ggtaatgttt gtaacaactt tgttgaatga
    15961 ttgtcaaatt agatgattaa aaattaaata aatgttgtta tcgtgacctg gatcactgtt
    16021 caggataaaa cccgacaaac tatatgtagg ttaattgtaa tgattttgtg aacggcctat
    16081 actgccgcca ggtctccgga acaccctgca atcccgagcc acccagcgtt gtaacgtgtc
    16141 gttttcgcat ctggaagcag tgttttgcat gacgcgcagt tatagaaagg acgctgtctg
    16201 acccgcaagc agaccggagg aaggaaatcc cgacgtctcc aggtaacaga aagttaacct
    16261 ctgtgcccgt agtccccagg gaataataag aacagcatgt gggcgttatt catgataaga
    16321 aatgtgaaaa aacaaagacc tgttaatctg gacctacaga ccatccggtt ccccatcacg
    16381 gcgatagcgt ccattctcca tcgcgtttcc ggtgtgatca cctttgttgc agtgggtatc
    16441 ctgctgtggc ttctgggtac cagcctctct tcccctgaag gtttcgagca agcttccgcg
    16501 attatgggca gcttcttcgt caaatttatc atgtggggca tccttaccgc tctggcgtat
    16561 cacgtcgtcg taggtattcg ccacatgatg atggattttg gctatctgga agaaacattc
    16621 gaagcgggta aacgctccgc caaaatctcc tttgttatta ctgtcgtgct ttcacttctc
    16681 gcaggagtcc tcgtatggta agcaacgcct ccgcattagg acgcaatggc gtacatgatt
    16741 tcatcctcgt tcgcgctacc gctatcgtcc tgacgctcta catcatttat atggtcggtt
    16801 ttttcgccac cagtggcgag ctgacatatg aagtctggat cggtttctgc gcctctgcgt
    16861 tcaccaaagt gttcaccctg ctggcgctgt tttctatctt gatccatgcc tggatcggca
    16921 tgtggcaggt gttgaccgac tacgttaaac cgctggcttt gcgcctgatg ctgcaactgg
    16981 tgattgtcgt tgcactggtg gtttacgtga tttatggatt cgttgtggtg tggggtgtgt
    17041 gatgaaattg ccagtcagag aatttgatgc agttgtgatt ggtgccggcg gcgcaggtat
    17101 gcgcgcggcg ctgcaaattt cccagagcgg ccagacctgt gcgctgctct ctaaagtctt
    17161 cccgacccgt tcccataccg tttctgcgca aggcggcatt accgttgcgc tgggtaatac
    17221 ccatgaagat aactgggaat ggcatatgta cgacaccgtg aaagggtcgg actatatcgg
    17281 tgaccaggac gcgattgaat atatgtgtaa aaccgggccg gaagcgattc tggaactcga
    17341 acacatgggc ctgccgttct cgcgtctcga tgatggtcgt atctatcaac gtccgtttgg
    17401 cggtcagtcg aaaaacttcg gcggcgagca ggcggcacgc actgcggcag cggctgaccg
    17461 taccggtcac gcactgttgc acacgcttta tcagcagaac ctgaaaaacc acaccaccat
    17521 tttctccgag tggtatgcgc tggatctggt gaaaaaccag gatggcgcgg tggtgggttg
    17581 taccgcactg tgcatcgaaa ccggtgaagt ggtttatttt aaagcccgcg ctaccgtgct
    17641 ggcgactggc ggagcagggc gtatttatca gtctaccact aacgcccaca ttaacaccgg
    17701 cgacggcgtc ggcatggcta tccgcgcagg cgtaccggtg caggatatgg aaatgtggca
    17761 attccacccg accggcattg ccggtgcggg cgtactggtc accgaaggtt gccgtggtga
    17821 aggcggttat ctgctgaaca aacatggcga acgctttatg gagcgttatg cgccgaacgc
    17881 caaagacctg gcgggccgtg atgtggtggc gcgttcaatc atgattgaaa tccgtgaagg
    17941 tcgcggctgt gatggtccgt gggggccaca cgcgaaactg aaactcgatc acctgggtaa
    18001 agaagttctc gaatcccgtc tgccgggtat cctggagctt tcccgtacct tcgctcacgt
    18061 tgatccggtg aaagagccga ttccggttat cccaacctgt cactacatga tgggcggtat
    18121 tccgaccaaa gttaccggtc aggcgctgac tgtgaatgag aaaggcgaag atgtggttgt
    18181 tccggggctg ttcgcggtag gtgaaattgc ctgcgtatcg gtacacggtg ctaaccgtct
    18241 gggcggcaac tcgctgctgg acctggtggt ctttggtcgc gcagcgggtc tgcatctgca
    18301 agagtctatc gccgagcagg gcgcattgcg cgatgccagc gagtctgatg ttgaagcatc
    18361 tttggatcgt ctgaaccgct ggaacaataa tcgtaacggt gaagatccgg tggcgattcg
    18421 taaagcgctg caagaatgta tgcagcataa cttctcggtc ttccgtgaag gtgatgcgat
    18481 ggctaaaggg cttgagcagt tgaaagtgat ccgcgagcgt ctgaaaaatg cccgtctgga
    18541 tgacacttcc agcgagttca acacccagcg cgttgagtgc ctggaactgg ataacctgat
    18601 ggaaacggcg tatgcaacgg ctgtttctgc caacttccgt accgaaagcc gtggcgcaca
    18661 tagccgcttc gacttcccgg atcgtgatga tgaaaactgg ctgtgccact ccctgtatct
    18721 gccagagtcg gaatccatga cgcgccgaag cgtcaacatg gaaccgaaac tgcgcccggc
    18781 attcccgccg aagattcgta cttactaatg cggagacagg aaaatgagac tcgagttttc
    18841 aatttatcgc tataacccgg atgttgatga tgctccgcgt atgcaggatt acaccctgga
    18901 agcggaagaa ggtcgcgaca tgatgctgct ggatgcgctt atccagctga aagagaaaga
    18961 tcccagcctg tcgttccgcc gctcctgccg tgaaggtgtg tgcggttccg acggtctgaa
    19021 catgaacggc aagaatggtc tggcctgtat taccccgatt tcggcactca accagccggg
    19081 caagaagatt gtgattcgcc cgctgccagg tttaccggtg atccgcgatt tggtggtaga
    19141 catgggacaa ttctatgcgc aatatgagaa aattaagcct tacctgttga ataatggaca
    19201 aaatccgcca gctcgcgagc atttacagat gccagagcag cgcgaaaaac tcgacgggct
    19261 gtatgaatgt attctctgcg catgttgttc aacctcttgt ccgtctttct ggtggaatcc
    19321 cgataagttt atcggcccgg caggcttgtt agcggcatat cgtttcctga tcgatagccg
    19381 tgataccgag actgacagcc gcctcgacgg tttgagcgat gcattcagtg tattccgctg
    19441 tcatagcatc atgaactgcg tcagtgtatg tccgaagggg ctgaacccga cgcgcgccat
    19501 cggccatatc aagtcgatgt tgttgcaacg taatgcgtaa accgtaggcc tgataagacg
    19561 cgcaagcgtc gcatcaggca accagtgccg gatgcggcgt gaacgcctta tccggcctac
    19621 aagtcattac ccgtaggcct gataagcgca gcgcatcagg cgtaacaaag aaatgcagga
    19681 aatctttaaa aactgcccct gacactaaga cagtttttaa aggttccttc gcgagccact
    19741 acgtagacaa gagctcgcaa gtgaaccccg gcacgcacat cactgtgcgt ggtagtatcc
    19801 acggcgaagt aagcataaaa aagatgctta agggatcacg atgcagaaca gcgctttgaa
    19861 agcctggttg gactcttctt acctctctgg cgcaaaccag agctggatag aacagctcta
    19921 tgaagacttc ttaaccgatc ctgactcggt tgacgctaac tggcgttcga cgttccagca
    19981 gttacctggt acgggagtca aaccggatca attccactct caaacgcgtg aatatttccg
    20041 ccgcctggcg aaagacgctt cacgttactc ttcaacgatc tccgaccctg acaccaatgt
    20101 gaagcaggta aaagtcctgc agctcattaa cgcataccgc ttccgtggtc accagcatgc
    20161 gaatctcgat ccgctgggac tgtggcagca agataaagtg gccgatctgg atccgtcttt
    20221 ccacgatctg accgaagcag acttccagga gaccttcaac gtcggttcat ttgccagcgg
    20281 caaagaaacc atgaaactcg gcgagctgct ggaagccctc aagcaaacct actgcggccc
    20341 gattggtgcc gagtatatgc acattaccag caccgaagaa aaacgctgga tccaacagcg
    20401 tatcgagtct ggtcgcgcga ctttcaatag cgaagagaaa aaacgcttcc taagcgaact
    20461 gaccgccgct gaaggccttg aacgttacct cggtgcaaaa ttccctggcg caaaacgctt
    20521 ctcgctggaa ggcggtgacg cgttaatccc gatgcttaaa gagatgattc gccacgctgg
    20581 caacagcggc acccgcgaag tggttctcgg gatggcgcac cgtggtcgtc tgaacgtgct
    20641 ggtgaacgtg ctgggtaaaa aaccgcaaga cttgttcgac gagtttgccg gtaaacataa
    20701 agaacacctc ggcacgggtg acgtgaaata ccacatgggc ttctcgtctg acttccagac
    20761 cgatggcggc ctggtacatc tggcgctggc gtttaacccg tctcaccttg agattgtcag
    20821 cccggtcgtt atcggttctg ttcgtgcccg tctggacaga cttgatgagc cgagcagcaa
    20881 caaagtgctg ccaatcacca ttcacggtga cgccgcagtg accgggcagg gcgtggttca
    20941 ggaaactctg aacatgtcga aagcgcgtgg ttatgaagtt ggcggtacgg tacgtatcgt
    21001 tatcaacaac caggttggct tcaccacctc taacccgctg gatgcccgtt caacgccgta
    21061 ctgtactgat atcggtaaga tggttcaggc accgattttt cacgttaacg cggatgatcc
    21121 ggaagccgtt gcctttgtga cccgtctggc gctcgatttc cgtaacacct ttaaacgtga
    21181 tgtcttcatc gacctggtgt gctaccgccg tcacggccac aacgaagccg acgagccgag
    21241 cgcaacccag ccgctgatgt atcagaaaat caaaaaacat ccgacgccgc gcaaaatcta
    21301 tgctgacaag ctggagcagg aaaaagtggc gacgctggaa gatgccaccg agatggttaa
    21361 cctgtaccgc gatgcgctgg atgctggcga ttgcgtagtg gcagagtggc gtccgatgaa
    21421 catgcactct ttcacctggt cgccgtacct caaccacgaa tgggacgaag agtacccgaa
    21481 taaagttgag atgaagcgcc tgcaggagct ggcgaaacgc atcagcacgg tgccggaagc
    21541 ggttgaaatg cagtctcgcg ttgccaaaat ttatggcgat cgccaggcga tggctgccgg
    21601 tgagaaactg ttcgactggg gcggcgcgga aaacctcgct tacgccacgc tggttgacga
    21661 aggcattccg gttcgcctgt cgggtgaaga ctccggtcgc ggtaccttct tccaccgcca
    21721 cgcggtgatc cacaaccagt ctaacggttc cacttacacg ccgctgcaac acatccataa
    21781 cggccagggc gcgttccgtg tctgggactc cgtactttct gaagaagcag tgctggcgtt
    21841 tgaatacggt tatgccaccg cagaaccacg cactctgact atctgggaag ctcagttcgg
    21901 tgacttcgcc aacggtgcgc aggtggttat cgaccagttc atctcctctg gcgaacagaa
    21961 atggggccgg atgtgtggtc tggtgatgtt gctgccgcac ggttacgaag ggcaggggcc
    22021 ggagcactcc tccgcgcgtc tggaacgtta tctgcaactt tgtgctgagc aaaacatgca
    22081 ggtgtgcgta ccgtctaccc cggcacaggt ttaccacatg ctgcgtcgtc aggcgctgcg
    22141 cgggatgcgt cgtccgctgg tcgtgatgtc gccgaaatcc ctgctgcgtc atccgctggc
    22201 ggtttccagc ctcgaagaac tggcgaacgg caccttcctg ccagccatcg gtgaaatcga
    22261 cgagcttgat ccgaagggcg tgaagcgcgt agtgatgtgt tctggtaagg tttattacga
    22321 cctgctggaa cagcgtcgta agaacaatca acacgatgtc gccattgtgc gtatcgagca
    22381 actctacccg ttcccgcata aagcgatgca ggaagtgttg cagcagtttg ctcacgtcaa
    22441 ggattttgtc tggtgccagg aagagccgct caaccagggc gcatggtact gcagccagca
    22501 tcatttccgt gaagtgattc cgtttggggc ttctctgcgt tatgcaggcc gcccggcctc
    22561 cgcctctccg gcggtagggt atatgtccgt tcaccagaaa cagcaacaag atctggttaa
    22621 tgacgcgctg aacgtcgaat aaataaagga tacacaatga gtagcgtaga tattctggtc
    22681 cctgacctgc ctgaatccgt agccgatgcc accgtcgcaa cctggcataa aaaacccggt
    22741 gacgcagtcg tacgtgatga agtgctggta gaaatcgaaa ctgacaaagt ggtactggaa
    22801 gtaccggcat cagcagacgg cattctggat gcggttctgg aagatgaagg tacaacggta
    22861 acgtctcgtc agatccttgg tcgcctgcgt gaaggcaaca gcgccggtaa agaaaccagc
    22921 gccaaatctg aagagaaagc gtccactccg gcgcaacgcc agcaggcgtc tctggaagag
    22981 caaaacaacg atgcgttaag cccggcgatc cgtcgcctgc tggctgaaca taacctcgac
    23041 gccagcgcca ttaaaggcac cggtgtgggt ggtcgtctga cccgtgaaga tgtggaaaaa
    23101 catctggcga aagctccggc gaaagagtct gcaccggcag cggctgctcc ggcggcgcaa
    23161 ccggctctgg ctgcacgtag cgaaaaacgt gtcccgatga ctcgcctgcg taagcgtgtg
    23221 gcagagcgtc tgctggaagc gaaaaactcc accgccatgc tgaccacgtt caacgaagtc
    23281 aacatgaagc cgattatgga tctgcgtaag cagtacggtg aagcgtttga aaaacgccac
    23341 ggcatccgtc tgggctttat gtccttctac gtgaaagcgg tggttgaagc cctgaaacgt
    23401 tacccggaag tgaatgcttc tatcgacggc gatgacgtgg tttaccacaa ctatttcgac
    23461 gtcagcatgg cggtttctac gccgcgcggc ctggtgacgc cggtactgcg tgatgtcgat
    23521 accctcggca tggcagacat cgagaagaaa atcaaagagc tggcagttaa aggccgtgac
    23581 ggcaagctga cggttgaaga tctgaccggt ggtaacttca ccatcaccaa cggtggtgtg
    23641 ttcggttccc tgatgtctac gccgatcatc aacccgccgc agagcgcaat tctgggtatg
    23701 cacgctatca aagatcgtcc gatggctgtg aatggtcagg ttgagatcct gccgatgatg
    23761 tacctggcgc tgtcctacga tcaccgtctg atcgatggtc gcgaatccgt gggcttcctg
    23821 gtaacgatca aagagttgct ggaagatccg acgcgtctgc tgctggacgt gtagtagttt
    23881 aagtttcacc tgcactgtag accgacgtgg atagggtgtc ggtctacggt ttaaaagata
    23941 acgattactg aaggatggac agaacacatg aacttacatg aatatcaggc aaaacaactg
    24001 tttgcccgct atggcttacc agcaccggtg ggttatgcct gtactactcc gcgcgaagca
    24061 gaagaagccg cttcaaaaat cggtgccggt ccgtgggtag tgaaatgtca ggttcacgct
    24121 ggtggccgcg gtaaagcggg cggtgtgaaa gttgtaaaca gcaaagaaga catccgtgct
    24181 tttgcagaaa actggctggg caagcgtctg gtaacgtatc aaacagatgc caatggccaa
    24241 ccggttaacc agattctggt tgaagcagcg accgatatcg ctaaagagct gtatctcggt
    24301 gccgttgtag accgtagttc acgtcgcgtg gtctttatgg cctccaccga aggcggcgtg
    24361 gaaatcgaaa aagtggcgga agaaactccg cacctgatcc acaaagttgc gcttgatccg
    24421 ctgactggcc cgatgccgta tcagggacgc gagctggcgt tcaaactggg tctggaaggt
    24481 aaactggttc agcagttcac caaaatcttc atgggcctgg cgaccatttt cctggagcgc
    24541 gacctggcgc tgatcgaaat caacccgctg gtcatcacca aacagggcga tctgatttgc
    24601 ctcgacggca aactgggcgc tgatggcaac gccctgttcc gccagcctga tctgcgcgaa
    24661 atgcgtgacc agtcgcagga agatccgcgt gaagcacagg ctgcacagtg ggaactgaac
    24721 tacgttgcgc tggacggtaa catcggttgt atggttaacg gcgcaggcct ggcgatgggt
    24781 acgatggaca tcgttaaact gcacggcggc gaaccggcta acttcctgga cgttggcggc
    24841 ggcgcaacca aagaacgtgt aaccgaagcg ttcaaaatca tcctctctga cgacaaagtg
    24901 aaagccgttc tggttaacat cttcggcggt atcgttcgtt gcgacctgat cgctgacggt
    24961 atcatcggtg cggtagcgga agtgggtgtt aacgtaccgg tcgtggtacg tctggaaggt
    25021 aacaacgccg aactcggcgc gaagaaactg gctgacagcg gcctgaatat tattgcagca
    25081 aaaggtctga cggatgcagc tcagcaggtt gttgccgcag tggaggggaa ataatgtcca
    25141 ttttaattga taaaaacacc aaggttatct gccagggctt taccggtagc caggggactt
    25201 tccactcaga acaggccatt gcatacggca ctaaaatggt tggcggcgta accccaggta
    25261 aaggcggtac tacccacctc ggcctgccgg tgttcaacac cgtgcgtgaa gctgttgctg
    25321 ctactggcgc taccgcttct gttatctacg taccagcacc gttctgcaaa gactccattc
    25381 tggaagccat cgacgcaggc atcaaactga ttatcaccat cactgaaggc atcccgacgc
    25441 tggatatgct gaccgtgaaa gtgaagctgg atgaagcagg cgttcgtatg atcggcccga
    25501 actgcccagg cgttatcact ccgggtgaat gcaaaatcgg tattcagcct ggtcacattc
    25561 acaaaccggg taaagtgggt atcgtttccc gttccggtac actgacctat gaagcggtta
    25621 aacagaccac ggattacggt ttcggtcagt cgacctgtgt cggtatcggc ggtgacccga
    25681 tccctggctc taactttatc gacattctcg aaatgttcga aaaagatccg cagaccgaag
    25741 cgatcgtgat gatcggtgag atcggcggta gcgctgaaga agaagcagct gcgtacatca
    25801 aagagcacgt taccaagcca gttgtgggtt acatcgctgg tgtgactgcg ccgaaaggca
    25861 aacgtatggg ccacgcgggt gccatcattg ccggtgggaa agggactgcg gatgagaaat
    25921 tcgctgctct ggaagccgca ggcgtgaaaa ccgttcgcag cctggcggat atcggtgaag
    25981 cactgaaaac tgttctgaaa taaatatctg taataagaaa tagccctcgc cgcttccctc
    26041 tacaggaatg gcgaagggct gtcggtttcg acatggttgg ccatcgtatg atggcctttt
    26101 ttatgcctta aacggttaac cacggtcgaa atctgactgc aggcttgact gcatgaccac
    26161 agccactgtg gattgccgca accgaccgca agccacgaat aagaacgacg gcgaatttcg
    26221 aatcgttacc tgatgcactg gcttcccagc gtttgaccgt agcgttaagt aaatcaacaa
    26281 aagcgatgtt caccgctttt cgtcaagtcg tgccatttgt cagccaggat cgggtcttta
    26341 ccccggatat tgcagcatcg caaaaattct tagagggata tgctataaat caaaaataaa
    26401 atctttgttg attgtttatc ataatatcca cagatatgat aaatgatatc aatttaataa
    26461 ttttcactta atgtgattcc ggataattcg aaaggcgctg tgcttttcgg attattatta
    26521 tctgattaag atatttgtca c
//
LOCUS       DAZFAB010000059        26086 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZFAB010000059 DAZFAB010000000
VERSION     DAZFAB010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26086
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.059"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            107..1471
                     /locus_tag="ACHJWA_002797"
     CDS             107..1471
                     /locus_tag="ACHJWA_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4309357.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLRNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPANIAANEALKVRLLETEGIKEVLIAEEEHSAYVKIDSKVTNRFDVEQA
                     IRQA"
     gene            complement(1599..2090)
                     /gene="yajQ"
                     /locus_tag="ACHJWA_002798"
     CDS             complement(1599..2090)
                     /gene="yajQ"
                     /locus_tag="ACHJWA_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFO4309358.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2258..3169
                     /gene="panE"
                     /locus_tag="ACHJWA_002799"
     CDS             2258..3169
                     /gene="panE"
                     /locus_tag="ACHJWA_002799"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFO4309359.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAACRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRTLRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3132..3722
                     /gene="yajL"
                     /locus_tag="ACHJWA_002800"
     CDS             3132..3722
                     /gene="yajL"
                     /locus_tag="ACHJWA_002800"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFO4309360.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLIRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(3776..5224)
                     /gene="thiI"
                     /locus_tag="ACHJWA_002801"
     CDS             complement(3776..5224)
                     /gene="thiI"
                     /locus_tag="ACHJWA_002801"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFO4309361.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     DGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTG
                     QEVVEVETVNDFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFKNVKVYRP"
     gene            5430..5672
                     /gene="xseB"
                     /locus_tag="ACHJWA_002802"
     CDS             5430..5672
                     /gene="xseB"
                     /locus_tag="ACHJWA_002802"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFO4309362.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            5672..6571
                     /gene="ispA"
                     /locus_tag="ACHJWA_002803"
     CDS             5672..6571
                     /gene="ispA"
                     /locus_tag="ACHJWA_002803"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFO4309363.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKAQDLIDDARQSLKQ
                     LAEQSIDTSALEALADYIIQRNK"
     gene            6596..8458
                     /gene="dxs"
                     /locus_tag="ACHJWA_002804"
     CDS             6596..8458
                     /gene="dxs"
                     /locus_tag="ACHJWA_002804"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFO4309364.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDETLILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            8513..9487
                     /gene="yajO"
                     /locus_tag="ACHJWA_002805"
     CDS             8513..9487
                     /gene="yajO"
                     /locus_tag="ACHJWA_002805"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFO4309365.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFSRREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDMVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            9718..11289
                     /gene="espY3"
                     /locus_tag="ACHJWA_002806"
     CDS             9718..11289
                     /gene="espY3"
                     /locus_tag="ACHJWA_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector pentapeptide repeat protein EspY3"
                     /protein_id="HFO4309366.1"
                     /translation="MMKTITKQPILFTDVPVADLRNSMKQDLNQNLIERLWNKIRDFF
                     LDSDKQKAFKSIHKYINTLSVLNYNSALTPDPNFNIDATSDLDSYLKLDFDRLSPKQK
                     QTTLCCFWNKIASSLPEPYNSTIKHNIIFYKDGENLMIRGTISIVNEVVKTYSLPIEK
                     DDNGYYDFSGLYLAHSNISGKDPNKDPDIDFGIDMGNCNCSNVNFEHTYFYGVKFTNA
                     NCTNANFNNCRFKKCDLTNMNCTGAILDNAMIYGKEKEPEMQYPEADQIIQRITYQKS
                     DGNETKGMILTNCSCVKTTFNWADLSESDCQNVDFSEANLSNTILPDIVRMKGTKLYR
                     TDLFNPILKTEAESTEEKDISPLAKIILDYIESDKNPESLNFEEKSTVIKIKQDIDNF
                     IFYNQHLKKIFNRAMNLQEKISRKKYNEFFKYIQAEAKQYFKDQYKLTKNDYLKKVPL
                     TAQLIAKYKMDDQLDQLLVTREIQDEIKSKIQDKIDELSKNLFNTMTETIENNFDDIF
                     RQQSENMSNYYEFVD"
     gene            complement(11467..11982)
                     /gene="pgpA"
                     /locus_tag="ACHJWA_002807"
     CDS             complement(11467..11982)
                     /gene="pgpA"
                     /locus_tag="ACHJWA_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFO4309367.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGIL"
     gene            complement(11960..12937)
                     /gene="thiL"
                     /locus_tag="ACHJWA_002808"
     CDS             complement(11960..12937)
                     /gene="thiL"
                     /locus_tag="ACHJWA_002808"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFO4309368.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVVPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            complement(13015..13434)
                     /gene="nusB"
                     /locus_tag="ACHJWA_002809"
     CDS             complement(13015..13434)
                     /gene="nusB"
                     /locus_tag="ACHJWA_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFO4309369.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(13454..13924)
                     /gene="ribE"
                     /locus_tag="ACHJWA_002810"
     CDS             complement(13454..13924)
                     /gene="ribE"
                     /locus_tag="ACHJWA_002810"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFO4309370.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(14013..15116)
                     /gene="ribD"
                     /locus_tag="ACHJWA_002811"
     CDS             complement(14013..15116)
                     /gene="ribD"
                     /locus_tag="ACHJWA_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFO4309371.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDVQTQAL
                     YPQHHLRQPVRIVIDSQNRVTPEHHIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(15120..15569)
                     /gene="nrdR"
                     /locus_tag="ACHJWA_002812"
     CDS             complement(15120..15569)
                     /gene="nrdR"
                     /locus_tag="ACHJWA_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFO4309372.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            15720..16259
                     /locus_tag="ACHJWA_002813"
     CDS             15720..16259
                     /locus_tag="ACHJWA_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFO4309373.1"
                     /translation="MNINVFRLILLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            16558..17442
                     /gene="tsx"
                     /locus_tag="ACHJWA_002814"
     CDS             16558..17442
                     /gene="tsx"
                     /locus_tag="ACHJWA_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFO4309374.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(17619..17966)
                     /gene="yajD"
                     /locus_tag="ACHJWA_002815"
     CDS             complement(17619..17966)
                     /gene="yajD"
                     /locus_tag="ACHJWA_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFO4309375.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(18054..18335)
                     /locus_tag="ACHJWA_002816"
     CDS             complement(18054..18335)
                     /locus_tag="ACHJWA_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO4309376.1"
                     /translation="MYKLSGKAVEDFRGIYDYTLGKFGDEQADRYTDSLGTFLDTLSQ
                     MPEIGQDYDAIPEVKKIAFRFHTVYYVIRVDDILIARILHQLMEPRRHW"
     gene            complement(18328..18570)
                     /locus_tag="ACHJWA_002817"
     CDS             complement(18328..18570)
                     /locus_tag="ACHJWA_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFO4309377.1"
                     /translation="MRKITSVSVGEQLDSFITRMVQSGRYGSASEVMRSALRLLEQQE
                     SRDEAVRNAVIEGLESGESSMTLRDIAAERKQKHRV"
     gene            complement(18722..19693)
                     /gene="secF"
                     /locus_tag="ACHJWA_002818"
     CDS             complement(18722..19693)
                     /gene="secF"
                     /locus_tag="ACHJWA_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFO4309378.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(19704..21551)
                     /gene="secD"
                     /locus_tag="ACHJWA_002819"
     CDS             complement(19704..21551)
                     /gene="secD"
                     /locus_tag="ACHJWA_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFO4309379.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(21579..21911)
                     /gene="yajC"
                     /locus_tag="ACHJWA_002820"
     CDS             complement(21579..21911)
                     /gene="yajC"
                     /locus_tag="ACHJWA_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFO4309380.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(21934..23061)
                     /gene="tgt"
                     /locus_tag="ACHJWA_002821"
     CDS             complement(21934..23061)
                     /gene="tgt"
                     /locus_tag="ACHJWA_002821"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFO4309381.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(23116..24186)
                     /gene="queA"
                     /locus_tag="ACHJWA_002822"
     CDS             complement(23116..24186)
                     /gene="queA"
                     /locus_tag="ACHJWA_002822"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFO4309382.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            24279..24860
                     /gene="acpH"
                     /locus_tag="ACHJWA_002823"
     CDS             24279..24860
                     /gene="acpH"
                     /locus_tag="ACHJWA_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFO4309383.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYAALETRFWQFYPRMMAQASRKAL"
     gene            complement(24881..>26086)
                     /locus_tag="ACHJWA_002824"
     CDS             complement(24881..>26086)
                     /locus_tag="ACHJWA_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4309384.1"
                     /translation="HPDKILPILHGCPVESIEVIKNAKRTGQWSYEMTDKNGQSIEVS
                     YIFTDTIDDKSDGKLCLKICNSCNQNDSSTHEYKIDHQQLKNHIRQLEEWALLEKFYD
                     NNAYYSKRTKQLEKKIADAGFITRKVKAENFNVKEFKDDIASMLCEQPPMRTHQISSS
                     GYVSADKLHKLYKTNLEDAQRDLKTTPVDIDGVSLKSITREKIAEEIAKKTAEIIAKQ
                     NAKSIEEKNIEKNKKQTNSEDNFVLKFSPEEINEMLLDYSDEEQKKLRPFFTSDHIYC
                     LFDAFSQGLHNVARNGSPRYAGLLRQIASKSAILQDFDQQLFWRTFTGGVLHRHISVK
                     SEPEKTDVWRFSSQFDFPHINENGELSRTVLMFEGVAESNFKDIANHSSECFSYRCYA
                     APLDANAKK"
BASE COUNT         6625 a         6707 c         6333 g         6421 t
ORIGIN      
        1 tagcattaga aacatatccc tctcgaaata tttactaaaa aatccgcatg tttaccccat
       61 tcgtttgccg ctttacacta gtcgcgaatt taaaacagag gtggtaatga acgattataa
      121 aatgacgcca ggtgagcggc gcgcgacctg gggtttaggg accgtattct cgttgcgcat
      181 gctgggcatg ttcatggttc tgccggttct gaccacgtac ggcatggctc tgcaaggtgc
      241 cagcgaagca ttaatcggta ttgccattgg tatttatggt ctgactcagg ccgtttttca
      301 gattccgttt ggcctgcttt ccgaccgcat tggtcgcaaa ccattaattg tcggtgggct
      361 ggcagtgttt gccgccggta gcgttatcgc tgcgctctct gactccatct ggggaattat
      421 tctgggtcgg gcgctacaag gctccggtgc gattgccgct gccgttatgg cgctgctttc
      481 cgatctcacg cgcgaacaaa accgcaccaa agcaatggcg tttatcggcg tgagctttgg
      541 cattaccttt gccattgcga tggtgcttgg cccgatcatc actcacaaac ttgggctgca
      601 cgcgctgttc tggatgatcg ctattctggc aacgaccggc attgcgttga ccatttgggt
      661 tgtgcccaac agtagcactc acgtacttaa tcgtgagtcc ggaatggtga aaggcagttt
      721 cagtaaggtg ctggcggaac cgcggctgct gaaactcaac tttggcatta tgtgtctgca
      781 tattttgctg atgtcgacgt ttgttgccct gcccggacaa ctggctgatg cggggttccc
      841 ggcggctgaa cactggaagg tctatctggc gacaatgcta atcgcctttg gctcggtcgt
      901 gcctttcatt atctacgctg aagttaagcg caaaatgaag caagtctttg tcttctgcgt
      961 cgggttgatc gtggttgcgg aaattgtgtt gaggaacgcg caaacgcagt tctggcaact
     1021 ggtggtcggc gtgcagcttt tctttgtggc gtttaatttg atggaagccc tcctgccctc
     1081 acttatcagt aaagagtcgc cagcaggtta caaaggtacg gcgatgggtg tttactccac
     1141 cagccagttt cttggcgtgg cgattggcgg ttcgctgggc ggctggattg acggcatgtt
     1201 tgacggtcag ggggtatttc tcgctggcgc aatgctggcc gcagtgtggc tggcagtcgc
     1261 cagtaccatg aaagaaccgc cgtatgtcag cagtttgcgc attgaaatcc cggcgaacat
     1321 tgccgcaaac gaggcgttaa aagtgcgttt gctggaaact gaaggcatca aagaagtgtt
     1381 gattgcagaa gaagaacatt cagcttatgt gaaaatcgac agcaaagtga cgaatcgctt
     1441 tgatgttgaa caggcaattc gccaggcata aaagcgaggc gaaattgcct gctgcgctac
     1501 gcttatcagg cctactaagt tcctgattta caaaactttg taggccgaac caggcgttca
     1561 tgccgcatct gacatgaaca aagcgcaggt tgtcgcgatt aatcgcggaa gtttttgaac
     1621 tggaacggct gaccgagatc gccaccgcgc accatcgcca tgacagcctg taaatcatca
     1681 cgagatttgc ccgtcacacg gatctcatcg ccctgaattt gcgcctgcac tttcagtttg
     1741 ctgtctttga tcatcttgac gatttttttc tgagtggcgc tctcaatgcc ctgtttcagt
     1801 ttcgcttcca caaaccaggt tttaccgcta tgaacgatat tttccggtac atccagtgaa
     1861 ctgccttcaa tgccgcgctt cagcagcttg gcacgcagaa tatccagcaa ctgattgacc
     1921 tggaagtcgg actcgctcaa cactttgatg gttttgctgg cgtcgttcag ctcaaatgag
     1981 gcttcaacgt tacggaagtc aaaacgggac tccacttcgc ggctcgcgtt atcgaccgcg
     2041 ttacgtgctt cctgaagatc aacttcagag acaatatcga aagatggcat cttttcctct
     2101 cccttcattt ttgatgcgaa gcataatacc tgcaaagtta aataaccaac agtcctgcac
     2161 caggacgtat cccttaattg tgcatagtcg caacagcatg cacaattaag ggatagcctt
     2221 attgaacacc cggagtggtt gcgggtgagg aggaacaatg aaaattaccg tattgggatg
     2281 cggtgcctta gggcaattat ggcttacagc actttgtaaa cagggtcacg aagttcaggg
     2341 ctggctgcgc gtaccgcaac cttattgtag tgtgaatctg gttgagactg atggttcgat
     2401 atttaacgaa tcgctgaccg ccaacgatcc cgattttctt gccaccagcg atctgctcct
     2461 ggtgacgctg aaagcatggc aggtttccga tgccgtcaaa agcctcgcgt ccacactgcc
     2521 tgtaactacg ccaatactgt taattcacaa cggcatgggc accatcgaag aattgcaaaa
     2581 cattcagcag ccattactga tgggcaccac cacccatgcc gcctgccgcg acggcaatgt
     2641 cattattcat gtggcaaacg gtatcacgca tattggtcca gcacggcaac aggacggcga
     2701 ttacagttat ctggcggata tattgcaaac cgtgttgcct gacgttgcct ggcataacaa
     2761 tattcgcgcc gagctgtggc gcaagctggc agtcaactgt gtgattaatc cactgactgc
     2821 catctggaat tgcccgaacg gtgaattacg tcatcatccg caagaaatta tgcaaatatg
     2881 cgaagaagtc gcggcagtga tcgaacgcga agggcatcat acttcagcgg aagatttacg
     2941 tgattacgtg atgcaggtga ttgatgccac agcggaaaat atctcgtcga tgttgcagga
     3001 tatccgcacg ctgcgccaca ccgaaatcga ctatatcaat ggttttctct tacgccgcgc
     3061 ccgcgcgcat gggattgccg taccggaaaa cacccgcctg tttgaaatgg taaaaagaaa
     3121 ggagagtgaa tatgagcgca tcggcactgg tttgcctcgc ccctggtagt gaagagactg
     3181 aagccgtcac cacgatcgac ctcttgattc gcggcggtat caaagtcacc actgccagcg
     3241 tagccagcga tggcaacctg gcgattacct gctcgcgcgg cgtgaagctg ctggcggatg
     3301 cgccgctggt cgaagtggct gatggcgaat atgacgtgat cgtgctgcct ggtggcatta
     3361 aaggcgcgga gtgttttcgc gatagtaccc tgctggttga aaccgttaaa cagttccacc
     3421 gttccgggcg tatcgtcgcg gctatttgcg ccgcgccagc caccgtgctg gtgccgcacg
     3481 atatctttcc gattggcaat atgaccggct tcccgacgct gaaagacaaa attcccgctg
     3541 aacaatggca ggacaagcgc gtcgtctggg atgcacgggt aaaattgctg accagccagg
     3601 ggccgggtac agctatcgac tttggtctga aaattatcga cctgctggtt gggcgcgaga
     3661 aagcccatga agtggcatca caactggtga tggcggcagg gatttataat tattacgagt
     3721 agtgtcggat gcggcaaacg tcgcatctga ccagatgcga cgtaaaaacc acaaattacg
     3781 gacgatacac cttcacattc ttaaagccct gttcgcgtag atagagcgcc tgcaggcggc
     3841 tcattacccc gcgctcacac cacagtagcc aggttctgtt ctggtcgaga tcgccaaatt
     3901 tggtgctcaa cttgtagaac ggcagtgaaa ccacatcaat cccttcgact ttcagtggct
     3961 tatcttcctg ttcatcgaca gaacggatat cgagaatcac gtcgttcggg ccgaagtcat
     4021 tgacggtttc cacttccacc acttcctgcc cggtctgctg ggcgatttcg cggatatcaa
     4081 cgttattcgc ttcttcaacc actttatcga gaatgctgaa gtcgaatttc tcttcttccg
     4141 cttcaatctt cgacttaacc gctttcaccg tcgggctttt ggagatcaca ccgcagtatt
     4201 ccggcatagt acgggcaaag tcttcggtgc caatctggcg agccaggttg atgatgtgct
     4261 ctttgtcgta agagatcagc ggacgcagga tcagcgtatc agagacgtta tcaatcaggc
     4321 gcaggttggt cagcgtctga ctggacacct ggccgagcgc ttcgccggtg accagcgcct
     4381 gtacgccgta acgttcagcc actttagacg cggcacgcac catcatacgt ttgaggataa
     4441 cgcccatctg accgtcgtcg attttctcga gaatttcccc gactaccggc tcgaaattaa
     4501 tagcgacaaa acgcacgcgg tgggagctgc caaagcggtt ccacagataa tgtgccacct
     4561 gacgcacgcc aatttcatgc gctgcgccgc cgaggttaaa gaagcagtaa tgcacgcggc
     4621 agccgcgacg catcaacata taactggaaa caccggagtc gaaaccaccg gaaatgagcg
     4681 acagcacatc ttcctgggtg ccgatcggga aaccgccaat accttcgtag cggcctttaa
     4741 tcagcaggag acgatcgtct tccacttcca gatggacagt cacatccgga ttggtcagct
     4801 tcacgcgcgc ggattcaata tgctgattta aaccgccgcc gacgtaacgt tccacatcaa
     4861 tcgagctaaa atcatgtttg ccacggcgct tcacgcgtac gcagaaggtt ttgccatcca
     4921 gctgatcgcg atactgaacc aacgctttct cgaaaatatc gtgcatgtcg gtaaacggca
     4981 cgtcttcgac ttcgagaata tggtggatac caggaatacg ggtcagggcg tcgcgaatag
     5041 tcagacgctg gttttcatct ttcgcgcgaa cttcgatgtt atcccagtgg cggacgacag
     5101 cgagcgtctc atcatagtgc tttaaaacgt tacgaatgtt cccggtaagg atttttataa
     5161 agcgcaagcg cacagattgg cttttgatgg tgatttccgg gaacaattta atgataaact
     5221 tcatggcggc aatggttcgt tggcaagcct taagcgactt gtatagggaa aaatacagca
     5281 gcccacacct gcggctgcat ccaggcgcgg aagtatacca ctaacatcgc tttgctgcgc
     5341 acatcacctt accattgcgc gttatttgct atttgccctg agtccgttac catgacgggg
     5401 cgaaaaatat tgagagtcag acattcatta tgccgaagaa aaatgaggcg cccgccagct
     5461 ttgaaaaggc gctgagcgag ctggaacaga ttgtaacccg tctggaaagt ggcgacctgc
     5521 cgctggaaga ggcgctgaac gagttcgaac gcggcgtgca gctggcacgt caggggcagg
     5581 ccaaattaca acaagccgaa cagcgcgtac aaattctgct gtctgacaac gaagacgcct
     5641 ctctaacccc ttttacaccg gacaatgagt aatggacttt ccgcagcaac tcgaagcctg
     5701 cgttaagcag gccaaccagg cgctgagccg ttttatcgcc ccactgccct ttcagaacac
     5761 tcccgtggtc gaaaccatgc agtatggcgc attattaggt ggtaagcgcc tgcgaccttt
     5821 cctggtttat gccaccggtc atatgttcgg cgttagcaca aacacgctgg acgcacccgc
     5881 tgccgccgta gagtgtatcc acgcttactc attaattcat gatgatttac cggcgatgga
     5941 tgatgacgat ctgcgtcgcg gcttgccgac ctgccatgtg aagtttggcg aagcgaacgc
     6001 gattctcgct ggcgacgctt tacaaacgct ggcgttctcg attttaagcg atgctgatat
     6061 gccagaagtg tcggatcgcg acagaatttc gatgatttct gaactggcga gcgccagcgg
     6121 tattgccgga atgtgtggtg gtcaggcgtt ggatttagac gcggaaggca aacacgtacc
     6181 tctggacgcg cttgagcgta ttcatcgtca taaaacaggc gcattaattc gcgccgccgt
     6241 tcgccttggt gcattaagcg ccggagataa aggacgtcgt gctctaccag tactcgacaa
     6301 gtacgcagag agcatcggcc ttgccttcca ggttcaggat gacatcctgg atgtggtggg
     6361 agatactgca acgttgggaa aacgtcaggg tgccgaccag cagcttggca aaagtaccta
     6421 ccctgcactc ctgggtcttg agcaagcccg gaagaaagcc caggatctga tcgacgatgc
     6481 ccgccagtcg ctgaaacaac tggctgaaca gtcgatcgat acttcggcac tggaagcgct
     6541 agcggactac atcatccagc gtaataaata aacaataagt gttaataggc ccctgatgag
     6601 ttttgatatt gccaaatacc cgaccctggc actggtcgac tccacccagg agctacgact
     6661 gttgccaaaa gagagtttac cgaaactctg cgacgaactg cgccgctatt tactcgacag
     6721 cgtgagccgt tccagcgggc acttcgcctc cgggctgggc acggtcgaac tgaccgtggc
     6781 gctgcactat gtctataaca ccccgtttga ccaattgatt tgggatgtgg ggcatcaggc
     6841 ttatccgcat aaaattttga ccggacgccg cgacaaaatc ggcaccatcc gtcagaaagg
     6901 cggtctacac ccgttcccgt ggcgcggcga aagcgaatat gacgtattaa gcgtcgggca
     6961 ttcatcaacc tccatcagtg ccggaattgg tattgcggtt gctgccgaga aagaaggcaa
     7021 aaatcgccgc accgtctgtg tgattggcga cggcgcgatt accgcaggca tggcgtttga
     7081 agcgatgaat cacgcgggcg atattcgtcc tgatatgctg gtggtcctca acgacaatga
     7141 aatgtcgatt tccgaaaatg ttggcgcgct taacaaccat ctggcgcagc tgctttccgg
     7201 taagctttac tcttcactgc gcgaaggcgg gaaaaaagtt ttctctggcg ttccgccaat
     7261 taaagagctg ctcaaacgta ccgaagaaca tattaaaggc atggtggtgc ctggcacgtt
     7321 gtttgaagag ctgggcttta actacatcgg cccggttgac ggtcacgatg tgctggggct
     7381 tatcaccacg ctgaagaaca tgcgcgacct gaaaggcccg cagttcctgc atatcatgac
     7441 caaaaaaggt cgtggttatg aaccggcaga aaaagaccca atcaccttcc acgccgtgcc
     7501 taaatttgat ccctccagcg gttgtttgcc gaaaagtagc ggtggtttac caagctattc
     7561 aaaaatcttt ggcgactggt tgtgcgaaac ggcagcgaaa gataacaaac tgatggcgat
     7621 tactccggcg atgcgtgaag gttccggcat ggtcgagttt tcacgtaaat tcccggatcg
     7681 ctattttgat gtagcgattg ccgagcaaca cgcggtgacc tttgccgccg gtctggcgat
     7741 tggtggctac aaaccgatcg tggcaattta ctccaccttc ctgcaacgcg cctatgatca
     7801 ggtgctgcat gacgtggcaa ttcaaaaact cccggtcctg ttcgccatcg accgcgcggg
     7861 cattgttggt gctgacggtc aaacccacca gggcgctttt gatctctctt acctgcgctg
     7921 cataccggaa atggtcatta tgaccccgag cgatgaaaac gaatgtcgcc agatgctcta
     7981 taccggctat cactataacg atggcccgtc agcggtgcgc tacccgcgtg gcaacgcggt
     8041 cggcgtggaa ctgacgccgc tggaaaaact accaattggc aaaggcattg tgaagcgtcg
     8101 tggcgagaaa ctggcgatcc ttaactttgg tacgctgatg ccagaagcgg cgaaagtcgc
     8161 cgaatcgctg aacgccacgc tggtcgatat gcgttttgtg aaaccgcttg atgaaacgtt
     8221 aattctggaa atggccgcca gtcatgaagc actggttacc gtagaagaaa acgccattat
     8281 gggcggtgca ggcagcggag tgaacgaagt gctgatggcc catcgtaaac cagtacccgt
     8341 gctgaacatt ggcctgccgg acttctttat tccgcaagga actcaggaag aaatgcgcgc
     8401 cgaactcggc ctcgatgccg ccggtatgga agccaaaatc aaggcctggc tggcataatc
     8461 cctactccac tcctgctatg cttaagaaat tattcatggc aggagtggca gcatgcaata
     8521 caacccctta ggaaaaaccg accttcgcgt ttcccgactt tgcctcggct gtatgacctt
     8581 tggcgagcca gatcgtggta atcacgcatg gacactgccg gaagagagca gccgtcccat
     8641 cattaaacgc gcactggaag gcggcataaa tttctttgat accgccaata gctattccga
     8701 tggcagcagc gaagagatcg tcggtcgcgc actgcgggat ttctcccgtc gtgaagacgt
     8761 ggtcgttgcc accaaagtgt tccatcgcgt tggtgattta ccggaaggat tatcccgtgc
     8821 acaaattttg cgctctatcg acgacagcct gcgacgtctc ggcatggatt atgtcgatat
     8881 cctgcaaatt catcgctggg attacaacac gccgatcgaa gagacgctgg aagccctcaa
     8941 cgacatggta aaagccggga aagcgcgtta tatcggcgcg tcatccatgc acgcttcgca
     9001 gtttgctcag gcactggaac tacaaaaaca gcacggctgg gcgcagtttg tcagtatgca
     9061 ggatcactac aatctgattt atcgcgaaga agagcgcgag atgctgccgc tgtgttatca
     9121 ggaaggcgtg gcggtcattc cgtggagtcc gctggcgcgg gggcgactga cgcgtccgtg
     9181 gggagaaact accgcacgac tggtgtctga tgaggtgggg aaaaatctct ataaagaaag
     9241 cgatgaaaat gacgcgcaga tcgcagagcg gttaacgggc gtcagtgaag aactcggtgc
     9301 aacacgagca caagttgcgc tggcctggtt gttgagtaaa ccgggcattg ccgcaccgat
     9361 tatcggaact tcgcgggaag aacagcttga tgagctgctg aacgcggtgg atatcacttt
     9421 gaagccggaa cagattgctg aactggaaac accgtataaa ccgcatccgg tcgtaggatt
     9481 taagtaaatt tcgcttttca cccattttat ctgctgaatt ccccatcgtc tgcgatgatg
     9541 gggacaacgt acctcccatc accttctgta accataatcc acctctgcaa gagaaattct
     9601 tcgtgaatga gtttgcagct attcatcgtc ttcatccctt tgagcgattt ttgatagttt
     9661 ctccaggtaa tatcccctaa aactgtctta tttagtcaac gtaagaggat tacaatcatg
     9721 atgaaaacca tcaccaaaca accgatcctg tttactgatg taccggttgc agatttaaga
     9781 aactcaatga agcaagattt aaatcaaaat cttattgaaa ggctgtggaa caaaattcgt
     9841 gatttttttc tggacagtga taaacaaaag gcatttaaat cgattcataa atacatcaat
     9901 acattgtcag ttctaaatta taattcagcg ttaactccag atcccaactt taatatagat
     9961 gcaacgtcag atttagactc atatttaaag ctggattttg atcgcctctc gcccaaacaa
    10021 aaacagacca cgctttgttg tttctggaat aaaattgcat cctcattgcc agagccatac
    10081 aacagtacca tcaaacataa cattattttt tataaagacg gagaaaatct gatgatacgt
    10141 ggaactatct ctattgttaa cgaggtagta aagacttatt cactcccaat agaaaaggat
    10201 gacaatggat attacgattt cagcggtcta tatctagcac acagcaatat ttccggtaaa
    10261 gatcccaata aagatcccga tatagatttc ggtatagata tgggaaactg taactgctca
    10321 aatgtcaact tcgaacatac ttatttttat ggcgtcaagt ttacaaatgc gaattgtaca
    10381 aatgcgaatt ttaacaattg tagatttaag aaatgcgacc tgacaaatat gaattgcact
    10441 ggcgcaattc ttgataatgc gatgatatat ggtaaagaaa aagaacctga gatgcaatat
    10501 ccagaagcag atcaaataat acaaaggata acttatcaga aatcagatgg gaatgaaacg
    10561 aaagggatga ttctaactaa ctgctcctgt gtgaaaacca ctttcaattg ggcagattta
    10621 tcagaaagtg attgccaaaa tgtggacttt agcgaggcta atctttcaaa cactattctt
    10681 cctgacatcg tcaggatgaa aggcacgaaa ctttaccgta ccgacctttt taatccgata
    10741 ttaaaaacag aagcagaatc aacagaagaa aaagacatta gcccattagc aaaaattatt
    10801 cttgactata ttgaatcaga taaaaaccct gagtcattaa actttgaaga aaaatcaaca
    10861 gttattaaaa taaaacaaga tatcgataat tttattttct acaatcagca tttaaagaaa
    10921 atattcaatc gcgcaatgaa tcttcaagaa aaaataagca ggaaaaagta taatgaattt
    10981 tttaaatata tacaagcaga agccaaacaa tatttcaaag accagtataa actaacaaaa
    11041 aatgattatt tgaagaaagt acctttaact gcacaattaa ttgcaaaata caaaatggat
    11101 gatcaattgg accaactttt ggttacccgt gaaattcaag atgaaataaa atcaaagatt
    11161 caagacaaaa tagatgaact ttctaaaaat ttattcaaca ctatgaccga aaccattgag
    11221 aataattttg atgatatttt tcgtcaacag tcagagaata tgagcaatta ttatgagttc
    11281 gtcgactgac gatatttcag tgtcattgaa ttatgagtga caaagataca ccttgaaata
    11341 tcgccgcata gtatattcgg cgatgtcggg ttttacttta acagagtgcc ccaaaattgg
    11401 ggcactgttc atttataaat caaaccgggt ggtctggaat cgcctacacc tcccggtcaa
    11461 tggcatttaa agaataccca gcggccagtg atgaccgata aagtacagga tgcctgcgga
    11521 aatcaccccg gcgacaatat catcgatcat gatccccatg ccgccatgta cattgcgatc
    11581 aaaccagcgg atcggccacg gcttccacat atccagaata cggaaaatca caaacccggc
    11641 ggcgacccac tgccagtcat tggtcggcag cgccatcagg gtgatccaca tgccaataaa
    11701 ctcatcccat acgatgctac cgtgatcgtg cacccccatg tctttcgccg tttgatgaca
    11761 aagatagacg ccgatacaga tccccaacat caccaccagc gagtagagct gccagggcaa
    11821 aaaggtcatc agataccaga acggaatcgc cgccagcgat cccatcgtgc caggaacgat
    11881 tgggcttaat ccacttccga atccgacagc aagcagatgc cacggattac tcatcttcag
    11941 gcgacttttc gcgacatctt tatggcgtgg caaaatggtc atatcctttc cagtctaacg
    12001 tgacaggttc gccgtcacga ataaaacaaa gcccttcgat atcggcggtc atttgcccga
    12061 tacaggtaaa cggtacgccc agatgaccga gagccacatc cagcgcgcca cggttcagtt
    12121 ccggcaccgt gaaacacaac tcgtaatctt caccgccaga gagcgcccag cgcagcgcct
    12181 gttccggtac aacatggcga gaaagcgcat cagaaaacgg cagcaatgcc aggtcaatac
    12241 gtgcgccgca gtcgctggct ttcacgatat gcccgagatc ggaaatcaga ccgtcagaga
    12301 gatcgatagc tgaatttgcc agatcgcgca gtgcctgccc ctgtaaaata cgcggcgatg
    12361 gacggagatg acgtttgatc aagtagtccg catctttagc atcggcaacc tgcaaacggt
    12421 tttgcaaaat cgccagcccg gcggcgctat cgcccggtgt accggtcaca tagatccagt
    12481 cacccggttt cgccccagag cgcgttaagg ctcgtcccat cggaacaaag ccgtggatac
    12541 ccaacgtcat tgataatggc ccacgcgtgg tatcgccgcc aatgagttgc atatcgtaat
    12601 aattgagaag atcaaacaaa ctgtcgctga aggactcaag ccacgcttcg tctacgtccg
    12661 gtaaggttaa tgccagcgtc agccaggccg gatcggcccc catcgctgcc agatcgctta
    12721 ggttcaccgc cagtgcttta taagccagat cagcaggatc gatatcaggg aggaaatggt
    12781 tgcccgccac cagcgtatca gtgctgatcg ccagggtctg tttctcgggg atattgagaa
    12841 gagcgcaatc gtcgccgatg cccagttcga catcaagacg agaacttctt acacggtcaa
    12901 aataacgggc aatcagggag aactcgccac atgccatacg ttatgcctca gcaggtaaaa
    12961 aagaaaaggc cggcgatcgc ggaacagtct tccgtgaatc taccggcctg gatattactt
    13021 tttgttaggg cgaatcacag gtgctgcttt atcgagtacg ccgttgacga acttatggct
    13081 gtcttctgcg ccgaacgatt tcgccagttc gatcgcttcg ttaatggcca ctttgtatgg
    13141 cacatcgcta cgtttagaca gttcgtacag cgcaatgcgc agtactgctt tttctacctg
    13201 tcccagttct tccagcaggc gggacaggta tggcttcatc agtccgtcga ggtatgcggt
    13261 attagtcgcc accccggcca gcagctcacg gaagtacagg acgtcaacgt cttttacatc
    13321 ctgttcagcc aggaactggt attcaacatc agcgatgtcg ttctgggaca actgccagga
    13381 gtagagcgcc tggacggcac actcacgagc gcggcgacga gcagcaggtt tcacggattt
    13441 ccccttacta atttcaggcc ttgatggctt tcaatacatt aatcatttca agcgcggtca
    13501 gtgcagcttc tgcacctttg ttgccagctt tggtgccagc acgttcgatc gcttgttcaa
    13561 tgctttcagt ggtcagaacc ccaaaagcaa ccggaatttc gctgtcctgg gcaacatgcg
    13621 ccaggccgtt gcttgcacca ccagcgacat attcaaaatg ggcagtgcca ccacgaataa
    13681 ccgtacccag cgcaatcacc gcgtcgtatt taccggtttt agccagtgca cccgccgcca
    13741 gcggcagctc ataggcacca ggcacccaaa caacggtaat gttttcatct tttacctgac
    13801 cgatacgttt cagtgcatca attgcacctt ccagcaggct gtcattgata aagttgttga
    13861 aacgcgcgat ggtgatggcg acgcgagcgt ccggggtagc aacgttagct tcaataatgt
    13921 tcattttctt ccttcgggtt cgagtatggc cccgcagggg ggcggatttt agcataatat
    13981 ttcgtgcgct gcttcccttt cgagccggga gatcatgcac ccactaaatg caggcaaaca
    14041 tccgggccta catgacgtat ctctttgaat ttaaattggg gggcgtcagc taatttctca
    14101 agccctggca gtgtgcataa tccacgggcg tcgctgccta atagtttagg tgcgatatag
    14161 acaatcagct catcgactaa ccccgcctgc agcaatgcgc cagcgagcgt tggccccgct
    14221 tccacccaga tgctgttaat ctgctgttta cccagttgca tcatcagtac aaccagatcc
    14281 agatgacctt tatgctctgg aatcagcaag gtacgcaccg tttccggcca ctcacgagaa
    14341 tcttcctgcg tacgcgcgaa ccaggtttcg ccgggctgct gcacaatgtg atgttccggc
    14401 gtcacgcgat tttggctatc aatcacaata cgtaccggct ggcggagatg atgttgcggg
    14461 tagagcgcct gagtttgtac atccagttca gaccaacgca ccgttaaggc aggatcatcc
    14521 gccagcaccg tagcgctgct ggttaaaatg gcatgacttt gtgcgcgcag tcgttgtacg
    14581 tcgcgccgcg cctgaggcga agtgatccac tggctttcgc cgctcgccat cgccgtgcgg
    14641 ccatcaagcg atgcgccaag ttttaactga atataaggaa agccggtgcg catccgcttg
    14701 agaaagcctt tattcaattg ctcggcttca ctcatcatca ggccgtggct gacgtcaatg
    14761 ccagcctgtt gcagacggta aagtccacgc ccagcgacct gcgggttcgg gtcctgcatc
    14821 gcagcgacca cgcgcgctac gccagcggcg atgagtgcgt cacagcacgg tggcgtacga
    14881 ccatgatggc tacagggttc gagtgtgaca taggcggtcg cacctttggc tttttcaccc
    14941 gccatacgca acgcgtgtac ttcggcatgt ggttcacccg ctcgttggtg gtaaccttca
    15001 ccgacaattt cgccatcttt gacaatgacg cacccgacat tcgggttggg gtgggtggta
    15061 aaacgtcctc gttgcgccag ctttagcgcc cgcgccatgt aatactcgtc ctgcacggct
    15121 tagtcctcca ggcgcgcgat ctcttcgcca aattctttga tatcttcgaa actgcggtag
    15181 acagaggcaa aacggatata ggcgacttta tcgagctttt tcaattgctc catcaccaga
    15241 ttgccaatca tcttgctcgg cacttcgcgc tcaccggtgg cgcgcagctg cgatttaata
    15301 tgattgattg ccatttcgac gtcatcggaa ctcaccggac gtttttccag cgcccgcagc
    15361 attccgctac gtaatttttc ttcattaaac ggttcacgca cgtcgttgct ttttacaaca
    15421 cgcggcataa ccagctccgc cacttcaaag gtggtgaaac gttcattaca caccagacac
    15481 tgccgacggc ggcgtacgga tgaaccctcg cccacgagac gagagtcaat taccttagtg
    15541 tccacggcga aacagaatgg gcaatgcata cggcgtcctg accaggtggt taacggaaat
    15601 ctattttacc ctgaactgac gatacaacaa aggcgcaacg cttttgagat aaaggatcga
    15661 tgctttcgct gcttttaccc tctaccatta ggctaattgc gacgagaaag ggacgaccta
    15721 tgaatattaa cgtttttcga ctgatcctgc tgggaagcct attcagcctt agcgcctgtg
    15781 tgcagcaaag tgaagtgcga cagatgaaac acagcgtcag cacgctgaac caggagatga
    15841 cgcaactcaa tcaagaaaca gtcaaaatca ctcagcaaaa caggctgaat gcaaaatcca
    15901 gcagtggggt ttaccttctg cctggagcga aaacaccggc aagactggaa agccagatcg
    15961 gtactttacg tatgtcgctg gtgaatatta cgcctgatac agatggcacc accctgacac
    16021 tacgtattca gggtgagtcg aatgatccct tgcccgcatt cagcggaact gttgaatatg
    16081 ggcaaattca gggaacaata gacaactttc aggaaatcaa tgtacagaat caattgatta
    16141 atgcccctgc cagcgtcctc gcccccagcg atgttgatat tccgttacag ttaaagggga
    16201 tttctgtgga tcagttaggc tttgtacgta tccacgacat tcaacctgtt atgcagtaaa
    16261 cttatagcgg ggcgattttg tgcgtcccgc aacatccttc cccatcattt tgttactccg
    16321 cttacataac ccagattgat agtaaaagtt tgcaacaagg gcgaaagtca gtacaatccc
    16381 cgcccgaatg tgtgtaaacg tgaacgcaat cgattacgta aatgatagaa ctgtgaaacg
    16441 aaacatattt ttgtgagcaa tgatttttat aataggctcc tctgtatacg aaatatttag
    16501 aaacgcaatt tgcgcctttt tcactcccgc aagggatttt caaacagtgg catacatatg
    16561 aaaaaaacat tactggcagc cggtgcggta ctggcgctct cttcgtcttt tactgtcaac
    16621 gcagctgaaa acgacaaacc gcagtatctt tccgactggt ggcaccagag cgttaacgtt
    16681 gtcggaagct atcacacccg tttcggaccg cagatccgca acgataccta ccttgagtac
    16741 gaagcattcg ctaaaaaaga ctggttcgac ttctatggtt atgcggatgc gccggtattc
    16801 ttcggcggta actccgatgc aaaaggtatc tggaaccacg gttctcctct gtttatggaa
    16861 atcgaaccac gtttctccat cgacaagctg accaatactg accttagctt cggtccgttc
    16921 aaagagtggt acttcgcgaa caactacatt tacgacatgg gtcgtaataa agatggtcgc
    16981 cagagcacct ggtacatggg tctgggtacc gatatcgaca ctggcctgcc gatgagcctg
    17041 tccatgaacg tctatgcgaa ataccagtgg cagaactatg gcgcagcgaa cgaaaacgag
    17101 tgggacggtt accgtttcaa aattaaatac tttgtgccga ttaccgatct gtggggcggt
    17161 cagctgagct acatcggctt caccaacttc gactggggtt ccgatttagg ggatgacagc
    17221 ggtaacgcaa tcaacggtat taagacccgt actaataact ctatcgcttc cagccatatt
    17281 ctggctctga actacgatca ctggcactac tctgtcgtag ctcgttactg gcacgacggt
    17341 ggtcagtgga acgacgatgc agaactgaac ttcggcaacg gcaacttcaa cgttcgctct
    17401 accggctggg gtggttacct ggtagtaggt tacaacttct gattatgaaa atgccgggat
    17461 ttattcccgg catttcagat tgttaacaac gtacgctttg ttcatgccgg atgcggcgta
    17521 aacgctttgt ccggcccaca aaagcacgta aattcaatat attgctgaaa taacgtaggc
    17581 ctgataagcg tagcgcatca ggcaatttta cgttttaatc acttcttctt gttcatcatc
    17641 gctttcaggt cagcgaatgg gttgtacttc gcttcaccga catctttctg cgcatcttcc
    17701 cctgcgataa cggtcgtacc atactgatcc gcttcggtat atttcgaatg ctcatgatcg
    17761 tggcaataga gacaaagcaa ttcccagtta ctgccatctt ccgggttatt ggtatggtcg
    17821 tgatcaatgt ggtgaacggt aagttcacgc aggttggaat aaacaaactc gcgggaacag
    17881 cgaccgcaga cccacggata gatttttaat gctttttcgc gatagccgct ttctaaccgc
    17941 gcatagtttt ttgggatgat agccatgaaa agttacctgg atgaaatgta gggttacgcg
    18001 cagtttccca agatgcgggg attttcgcaa tcctgagtca agaaattctc acattaccag
    18061 tgacggcgag gttccatcaa ctgatgaaga atgcgcgcaa tgagaatgtc atcgactctt
    18121 ataacgtaat aaacagtgtg aaacctaaat gcgatcttct ttacctctgg aatcgcatca
    18181 tagtcttgcc ctatctcggg catttgagag agtgtatcca gaaaagtgcc gagagagtcg
    18241 gtatagcgat ccgcctgctc atcaccaaac tttcccagcg tgtagtcata gatccctctg
    18301 aaatcttcaa cagcttttcc gctaagctta tacacgatgt ttttgcttcc tttctgctgc
    18361 gatatcacga agcgtcatcg aactttcacc gctctcaaga ccttcaataa cagcatttct
    18421 gacagcttca tccctggatt cttgttgttc caatagacga agtgccgaac gcattacttc
    18481 actggccgag ccataacgac cagactggac catccgggta ataaaactat ctaattgttc
    18541 gccaacgctt actgaggtaa ttttgcgcat aagcgatctc cacgtattaa ctattaatac
    18601 aaagtatatg tcagaacgct ttctacaacc aaaccgtatt cagttgcctg tgaaacaggc
    18661 aaaaaaaaac cgcagcgcaa atacgccacg gtttttttaa cagaacggat ttaaaacacc
    18721 attacggcag aattgacggc tgatccgccc cttctttttc cactttctgc tgcaacatgt
    18781 gttcgcgctt catacccagt ttcagagcca acgcagatgc cacatagatg gaagatgcag
    18841 taccgatgga aacaccgata agcatggtca gcgagaagcc ttccagtacc ggaccaccga
    18901 agagatacag catcaggata accatcaagg tagtaccgga tgtgatcaag gtacggtgca
    18961 gcgtctgggt caaggacacg ttaaagattt cgtaaggcgt accgcgacgg atcttgcgga
    19021 agttttcacg aattcggtcc gataccacga tactgtcgtt aagcgagtaa ccgataaccg
    19081 acatcaacga tgccacaatg gtcaggtcaa tctcgatatg gaataacgac aaaataccca
    19141 gcgtaatgat aacgtcgtgc gccagcgcaa taaccacccc tgccgccagt cgccactcaa
    19201 agcggaaacc tacgtacacg aggatagaca gcagcgctgc catcaacgcc atcgcaccgg
    19261 tttgcgcaag gtctgccccc acgctcggac cgacgaactc aatacgcttc actgctgcat
    19321 tctgattggt ggattcgtta atcaccttca gaacctggct gcccagcacc tgaccgccgg
    19381 tttcgccttc agcaggcggc atacggacca tgatgtcgtg gctgctacca aagttttgca
    19441 gcatcggctc ttcaaaaccg gctttttgca atgcatcacg cattacgtca atttcagccg
    19501 gtttttcgag cgtaatttcg ataaccgtac caccggtgaa atccagcccc cagttaaagc
    19561 cgcgcacgcc cataataacg atagcagcga ttaacagcag accagagatg ccaaaagccc
    19621 agtagtccca gcgcataaag tcatagactt tacggccgtg gtttagttgt tcaacagtat
    19681 attcctgtgc cacatcgcac tcctcagatt gacagcttct tgacgcgctt gccgccatat
    19741 agcaggttta cgatggcacg cgtaccgaca atcgcggtaa acatcgacgt cgccacaccg
    19801 ataccggtag taatcgcgaa ccctttaatt gccccggtac ccactgcgta caggatgatg
    19861 actttaatca gcgtggtgat gttcgcatcg aagatagaac tgaatgcgcc acgataacct
    19921 tcatcaattg cctgttgaac agtacgtccg ttgctcaact cttctttaat acgttcgttg
    19981 atcagtacgt tcgcatcgac cgctaccgca agggttaaga cgatacccgc gatacctggc
    20041 atactcagtg ttgcgcctgg caacagcgac ataataccga cgatcaagat caagttggca
    20101 atcagagcac tggtcgcaat cagaccaaac ttcttataga agatgatcat gaacagaata
    20161 gaaaccagca gaccggcaag gcaagcctcc agcccctgct caatgttctg catacccagg
    20221 gtcgggccaa ttgtacgttc ttcaacaatc tgaatcggcg cgatcaacgc accggcacgc
    20281 agcagcagtg acagctgacg ggcttcgttc gggttgttga tgccggtgat acggaagctg
    20341 ttacccagac gagactggat gttggcgatg ttaatcacct cttcctgttt caccagaacc
    20401 gcacgaccat ttgcatcttt cttaccgctg tctttgtact ccacaaacag ggttgccatc
    20461 ggtttgccga tgttgtcctt ggtgaagtta gacataatgt tgccaccagc gctatcgagc
    20521 gagatgttaa cctgcggctg gttgtattcg tcctggctgg aagtggagtc ggtgatatgg
    20581 tcaccggtaa gaattacgcg tttgtacagc acaaccggct gaccttcgcg ggtctgtttc
    20641 acttcagagt cgcccggtac gcgaccggat gccgcagcgg cctgatcaac gttggtgttt
    20701 accagacgga attccagcgt tgccgtcgca cccagaatct ctttcgcacg cgcagtgtcc
    20761 tgaatacctg gcagttcaac aacgatacgg tcagcaccct gacgctgaac caccggctcc
    20821 gccacgccaa gttggtttac acggttacgc aggatattga tgttctgctg caccgcatat
    20881 tcacgcgctt cactcagacg agcatcgctc attaccgcac gcagctggtt gctgccctga
    20941 ctgctaatca ccaggtccgg atgacgcttg ctcagatacg caatggcttc atcacgagct
    21001 ttagcatcgc ggaaagtgat gctcaggccg tagttatttt ctttacgaac ggtggtatac
    21061 gggatgcctt tttcgcgcag gtcgctgcgc aggctatcga tattttgttc ctggagtttg
    21121 ccaagcgcgg tgtccatatc cacttccatc aggaagtgaa cgccgccacg caggtcaagg
    21181 ccgagcttca tcggctcagc gtgaatagct gccagccagc gcggcgttgc cggggcaagg
    21241 ttaagcgcca cgacgtattt gtcacccata acgcccatta atgcttcacg agcgcgcagc
    21301 tgggtgtcag tggagtcgaa gcgcgcaaga atagcgccct cttccagtgc cacagactta
    21361 gcagttattt tttcttcttg taacgttttc tggacctgga tcagcgtttg ctcactggcg
    21421 gcgactccgc gcgcaccagt gatctgaaca gccggatcct caccaaacag gttgggaagc
    21481 gcatacagca gaccgatgac aatcaccacg atcagcatga cgtacttcca caaaggataa
    21541 cggtttaaca cggcaattcc cttagggaaa aattttaatt acagcgcctt catggtgcct
    21601 ttcggcagga cggcagctac gaagtcacgt ttaataacta cttcagtggt gtcattcagc
    21661 gcgatagcaa tgtagccgtt ttccgctact ttggttacgc gaccaaccag gccaccgttc
    21721 gtcagaactt catcaccttt ggcaatggag tccatcagct ttttgtgttc tttggtgcgc
    21781 ttctgctgtg gacgcaggat catgaaatag aaaatcagac cgaataccac cagcatcaaa
    21841 atcaaagaca tcgggctacc ttgcgccggt gcacccgttg ccgctaccgc atcagaaata
    21901 aaaaagctca ttaaatttcc ctcattatta atattaatca acgttcaaag gtggtacttc
    21961 tcgcccctga cgctggtaaa aatcagttac gaagctctct aatttaccct cttcaatagc
    22021 cttgcgtaaa cccgccatca aacgctggta gtaacgaagg ttatgaatgg tattgagtcg
    22081 cgcgcctaat atttcgttgc aacggtcaag atgatgcaag taagcgcgtg aataattgcg
    22141 acaggtgtag caatcacact caggatcgag tgggccagta tcgctcttat acttcgcatt
    22201 gcggattttc accacgccat cggtcacgaa caaatgacca tttcgggcgt tgcgggttgg
    22261 cattacgcag tcaaacatat cgataccacg acgtacgcct tcaaccaggt cttctggttt
    22321 accaacgccc atcaggtaac gcggtttgtc tgccggaatt tgcgggcata catgctccag
    22381 aatgcggtgc atatctgctt tcggctcacc cacagccaga ccgccgacag cgtagccatc
    22441 aaaaccgata tctaccagac ctttaacaga aatatcacgt aaatcttcgt aaacgctgcc
    22501 ctgaatgata ccaaacagcg catttttgtt tccgagactg tcaaaacgct cacggctacg
    22561 cttcgcccaa cgcagagaca tctccatgga gcgttttgcg taatcccagt cagcaggata
    22621 cggcgtacac tcatcaaaga tcatgacgat atccgaacca agatcgtact gaatctccat
    22681 cgacttttcc gggtcgagga aaatcgggtc gccgttgatc gggttacgga agtgcacgcc
    22741 ctgttcggtg attttacgaa tatcgccaag gctgaagact tggaagccgc cggagtcggt
    22801 gaggatcggt cccttccact gcataaaatc gtgcagatcg ccgtgcagtt tcatgatttc
    22861 ctggcccggg cgcagccaca ggtggaaggt gttgccgagg ataatttgcg cgccagtggc
    22921 ttcaacttct tccggcgtca tcccttttac ggtgccgtag gtgccaacag gcataaaaca
    22981 aggcgtttcc actacgccac gatcaaagac caggcggcca cggcgtgcgc gaccgtcggt
    23041 ggtgtccagt tcaaatttca ttttttctcc actacgtcag aaaaacagtc caacgtttaa
    23101 accagcgccg cggaattact ccccgacgcg ctcattaatt gcctgcggat tgtacgtgat
    23161 aaacatcgca tcaccgtaac taaaaaagcg atatttctct tctaccgccg ctttataggc
    23221 attcattgtg tgttgataac cggcaaaggc cgaaaccagc ataatcagcg tcgattccgg
    23281 aaggtggaag tttgtcacca gcgcatcgac cactttgtac tggaagcccg gatagataaa
    23341 gatttgggta tcgtcaaaga acggttcgat gagatcgttt ttcgccgcct gagccgcgct
    23401 ttccagcgaa cgtactgagg tggtgccaac cgcaatcacc cggttaccgc gcgctttcgc
    23461 cgccagtacc gcgtctacca catcctgcgg tacttcagcg tattccgagt gcatgatgtg
    23521 atcttcaatg gtgtcgacgc gtaccggctg gaaggtgccc gcaccaacgt gcagggtgac
    23581 gaacgccatc tccacgcctt tggcgcgtaa tttttccagc aaaggttcgt caaaatgcag
    23641 accagcggtc ggggctgcaa ccgcgcccgg tttttcgcta taaacggttt gataaagttc
    23701 gcggtcagcg tcttcgtccg gacggtcgat atacggcggc agcggcatat ggccgatgct
    23761 gttgagaata tccagcaccg agcgttcatc attaaattcg acttcaaaca gtgcgccgtg
    23821 gcgcgcggtc attgttgcgt taatactttc gtcatcgccc agcagcagtt ctgcgccagg
    23881 ttttggcgct ttcgaggcgc gaatatgcgc aagaatgcgt ttgtcgtcga gcatccgttc
    23941 aaccagcact tcaatcttgc cgccgctggc tttacgccca aacaggcgcg ccgggatcac
    24001 ccgggtatta ttaaaaacca gaagatcgcc ggggttgagc ttatcgagta aatcggtgaa
    24061 agtaccgtgc gtcagcgcgc ccgtcggccc gtccagcgac agtaaacgac agctactgcg
    24121 ttcaggcatc ggatagtggg caatcaggga ttcgggcaat tcaaaggaga aatcggtaac
    24181 gcgcatgaca ctgactcaga ctaaaataag aggcgggtag tctagtgccg gggcgctctc
    24241 cctgcaacct ttacccccct ttggatagaa tataatcgat gaatttttta gctcacctgc
    24301 atttagccca tctcgcggaa agctcgcttt ccggcaattt gctggctgat ttcgtacgcg
    24361 gaaatcctga agaaagtttt ccgcccgacg tcgtggctgg cattcatatg catcgacgta
    24421 tcgacgtatt gactgacaat ctgccggaag tccgcgaagc acgggagtgg tttcgtagtg
    24481 aaactcgccg cgttgcgcct attacgctgg atgtcatgtg ggatcacttt ctttcccgtc
    24541 actggtcgca gctgtcgccg gactttccgc tacaggaatt tgtttgttat gcccgcgagc
    24601 aagtgatgac aattttgccg gactcaccgc cacgctttat caacctgaac aactacttat
    24661 ggtcagagca gtggctggtg cgctatcgcg atatggattt catccagaac gtgttaaacg
    24721 gcatggcaag ccgtcgccca cgtctggatg ccctgcgtga ctcctggtac gatttagacg
    24781 cccattatgc cgccctcgaa acccgcttct ggcagtttta tccacggatg atggcgcagg
    24841 cgtcacgcaa ggcgctctga aacttcaaac tccggcgtca ctatttttta gcattcgcat
    24901 ccagcggggc ggcataacaa cggtaagaaa aacattcgct ggaatgattg gcgatatctt
    24961 taaagttgct ttcagcaacc ccctcgaaca tgagtaccgt tctggacaac tctccgtttt
    25021 catttatatg tggaaaatca aattggctac tgaatcgcca tacatctgtc ttttctggct
    25081 cgctcttcac agagatatgt cgatggagga cccctcctgt aaacgtgcgc cagaacaatt
    25141 gttgatcaaa atcttgtaat atcgcagact tggatgctat ttgacgtagt aaacctgcat
    25201 accgtggact gccattacga gcaacattat gcaaaccctg actaaaagca tcaaacaaac
    25261 aatagatgtg atcactagtg aaaaaaggtc ttaatttttt ttgttcttca tcgctgtaat
    25321 ccaggagcat ctcattgatt tcttccggtg agaattttaa gacaaaatta tcctcagaat
    25381 ttgtttgttt cttgtttttt tcaatattct tctcctcaat gctcttcgca ttttgtttcg
    25441 caattatttc cgcagttttt ttcgcaattt cttccgcaat tttttcccga gttattgatt
    25501 ttagactaac accatcgatg tcaaccggtg ttgttttcag atctctctgc gcgtcttcca
    25561 aatttgtctt atagagtttg tgcagtttat cggcagatac gtatccggat gaagaaatct
    25621 ggtgtgtacg catggggggc tgttcacaaa gcatcgaggc tatatcgtct ttaaactctt
    25681 ttacattaaa gttctcggct ttaaccttcc ttgttataaa ccccgcatcg gcaattttct
    25741 tctctaattg ttttgtacgt ttagaataat aagcgttgtt atcataaaac ttttcaagaa
    25801 gggcccactc ctcaagttgc cttatgtgat ttttcaactg ttgatgatct attttatatt
    25861 catgtgtact agaatcattt tggttgcagg agttacagat ttttaagcat aattttccat
    25921 cagatttatc atctatggta tcagtgaaaa tataggacac ttcaatactc tgcccgttct
    25981 tatcagtcat ctcatatgac cattgtcccg ttcgtttcgc attcttaatg acttcgatcg
    26041 actctacagg acaaccatga agaatgggca gaattttatc aggatg
//
LOCUS       DAZFAB010000060        26068 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZFAB010000060 DAZFAB010000000
VERSION     DAZFAB010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26068
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.060"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            198..392
                     /gene="bfd"
                     /locus_tag="ACHJWA_002825"
     CDS             198..392
                     /gene="bfd"
                     /locus_tag="ACHJWA_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFO4309385.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            465..941
                     /gene="bfr"
                     /locus_tag="ACHJWA_002826"
     CDS             465..941
                     /gene="bfr"
                     /locus_tag="ACHJWA_002826"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFO4309386.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVND"
     gene            complement(938..1405)
                     /locus_tag="ACHJWA_002827"
     CDS             complement(938..1405)
                     /locus_tag="ACHJWA_002827"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFO4309387.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR"
     gene            1784..2095
                     /gene="rpsJ"
                     /locus_tag="ACHJWA_002828"
     CDS             1784..2095
                     /gene="rpsJ"
                     /locus_tag="ACHJWA_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFO4309388.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2128..2757
                     /gene="rplC"
                     /locus_tag="ACHJWA_002829"
     CDS             2128..2757
                     /gene="rplC"
                     /locus_tag="ACHJWA_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFO4309389.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2768..3373
                     /gene="rplD"
                     /locus_tag="ACHJWA_002830"
     CDS             2768..3373
                     /gene="rplD"
                     /locus_tag="ACHJWA_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFO4309390.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3370..3672
                     /gene="rplW"
                     /locus_tag="ACHJWA_002831"
     CDS             3370..3672
                     /gene="rplW"
                     /locus_tag="ACHJWA_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFO4309391.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3690..4511
                     /gene="rplB"
                     /locus_tag="ACHJWA_002832"
     CDS             3690..4511
                     /gene="rplB"
                     /locus_tag="ACHJWA_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFO4309392.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4528..4806
                     /gene="rpsS"
                     /locus_tag="ACHJWA_002833"
     CDS             4528..4806
                     /gene="rpsS"
                     /locus_tag="ACHJWA_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFO4309393.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4821..5153
                     /gene="rplV"
                     /locus_tag="ACHJWA_002834"
     CDS             4821..5153
                     /gene="rplV"
                     /locus_tag="ACHJWA_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFO4309394.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5171..5872
                     /gene="rpsC"
                     /locus_tag="ACHJWA_002835"
     CDS             5171..5872
                     /gene="rpsC"
                     /locus_tag="ACHJWA_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFO4309395.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5885..6295
                     /gene="rplP"
                     /locus_tag="ACHJWA_002836"
     CDS             5885..6295
                     /gene="rplP"
                     /locus_tag="ACHJWA_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFO4309396.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6295..6486
                     /gene="rpmC"
                     /locus_tag="ACHJWA_002837"
     CDS             6295..6486
                     /gene="rpmC"
                     /locus_tag="ACHJWA_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFO4309397.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6486..6740
                     /gene="rpsQ"
                     /locus_tag="ACHJWA_002838"
     CDS             6486..6740
                     /gene="rpsQ"
                     /locus_tag="ACHJWA_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFO4309398.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6905..7276
                     /gene="rplN"
                     /locus_tag="ACHJWA_002839"
     CDS             6905..7276
                     /gene="rplN"
                     /locus_tag="ACHJWA_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFO4309399.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7287..7601
                     /gene="rplX"
                     /locus_tag="ACHJWA_002840"
     CDS             7287..7601
                     /gene="rplX"
                     /locus_tag="ACHJWA_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFO4309400.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7616..8155
                     /gene="rplE"
                     /locus_tag="ACHJWA_002841"
     CDS             7616..8155
                     /gene="rplE"
                     /locus_tag="ACHJWA_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFO4309401.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8170..8475
                     /gene="rpsN"
                     /locus_tag="ACHJWA_002842"
     CDS             8170..8475
                     /gene="rpsN"
                     /locus_tag="ACHJWA_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFO4309402.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8509..8901
                     /gene="rpsH"
                     /locus_tag="ACHJWA_002843"
     CDS             8509..8901
                     /gene="rpsH"
                     /locus_tag="ACHJWA_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFO4309403.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8914..9447
                     /gene="rplF"
                     /locus_tag="ACHJWA_002844"
     CDS             8914..9447
                     /gene="rplF"
                     /locus_tag="ACHJWA_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFO4309404.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9457..9810
                     /gene="rplR"
                     /locus_tag="ACHJWA_002845"
     CDS             9457..9810
                     /gene="rplR"
                     /locus_tag="ACHJWA_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFO4309405.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9825..10328
                     /gene="rpsE"
                     /locus_tag="ACHJWA_002846"
     CDS             9825..10328
                     /gene="rpsE"
                     /locus_tag="ACHJWA_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFO4309406.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10332..10511
                     /gene="rpmD"
                     /locus_tag="ACHJWA_002847"
     CDS             10332..10511
                     /gene="rpmD"
                     /locus_tag="ACHJWA_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFO4309407.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10515..10949
                     /gene="rplO"
                     /locus_tag="ACHJWA_002848"
     CDS             10515..10949
                     /gene="rplO"
                     /locus_tag="ACHJWA_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFO4309408.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10957..12288
                     /gene="secY"
                     /locus_tag="ACHJWA_002849"
     CDS             10957..12288
                     /gene="secY"
                     /locus_tag="ACHJWA_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFO4309409.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12320..12436
                     /gene="rpmJ"
                     /locus_tag="ACHJWA_002850"
     CDS             12320..12436
                     /gene="rpmJ"
                     /locus_tag="ACHJWA_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFO4309410.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12583..12939
                     /gene="rpsM"
                     /locus_tag="ACHJWA_002851"
     CDS             12583..12939
                     /gene="rpsM"
                     /locus_tag="ACHJWA_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFO4309411.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12956..13345
                     /gene="rpsK"
                     /locus_tag="ACHJWA_002852"
     CDS             12956..13345
                     /gene="rpsK"
                     /locus_tag="ACHJWA_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFO4309412.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13379..13999
                     /gene="rpsD"
                     /locus_tag="ACHJWA_002853"
     CDS             13379..13999
                     /gene="rpsD"
                     /locus_tag="ACHJWA_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFO4309413.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14025..15014
                     /gene="rpoA"
                     /locus_tag="ACHJWA_002854"
     CDS             14025..15014
                     /gene="rpoA"
                     /locus_tag="ACHJWA_002854"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFO4309414.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15055..15438
                     /gene="rplQ"
                     /locus_tag="ACHJWA_002855"
     CDS             15055..15438
                     /gene="rplQ"
                     /locus_tag="ACHJWA_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFO4309415.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15545..15913
                     /locus_tag="ACHJWA_002856"
     CDS             15545..15913
                     /locus_tag="ACHJWA_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFO4309416.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLNKINDN"
     gene            15924..16349
                     /gene="zntR"
                     /locus_tag="ACHJWA_002857"
     CDS             15924..16349
                     /gene="zntR"
                     /locus_tag="ACHJWA_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFO4309417.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16405..16623
                     /gene="arfA"
                     /locus_tag="ACHJWA_002858"
     CDS             16405..16623
                     /gene="arfA"
                     /locus_tag="ACHJWA_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFO4309418.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(16620..17030)
                     /gene="mscL"
                     /locus_tag="ACHJWA_002859"
     CDS             complement(16620..17030)
                     /gene="mscL"
                     /locus_tag="ACHJWA_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFO4309419.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17160..18536)
                     /gene="trkA"
                     /locus_tag="ACHJWA_002860"
     CDS             complement(17160..18536)
                     /gene="trkA"
                     /locus_tag="ACHJWA_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFO4309420.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18558..19847)
                     /gene="rsmB"
                     /locus_tag="ACHJWA_002861"
     CDS             complement(18558..19847)
                     /gene="rsmB"
                     /locus_tag="ACHJWA_002861"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFO4309421.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKSMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNTSDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRINRTHHSRDTWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(19893..20840)
                     /gene="fmt"
                     /locus_tag="ACHJWA_002862"
     CDS             complement(19893..20840)
                     /gene="fmt"
                     /locus_tag="ACHJWA_002862"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFO4309422.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWVEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(20855..21364)
                     /gene="def"
                     /locus_tag="ACHJWA_002863"
     CDS             complement(20855..21364)
                     /gene="def"
                     /locus_tag="ACHJWA_002863"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFO4309423.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21494..22618
                     /gene="dprA"
                     /locus_tag="ACHJWA_002864"
     CDS             21494..22618
                     /gene="dprA"
                     /locus_tag="ACHJWA_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFO4309424.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLARQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLAKHGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22590..23063
                     /gene="smg"
                     /locus_tag="ACHJWA_002865"
     CDS             22590..23063
                     /gene="smg"
                     /locus_tag="ACHJWA_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFO4309425.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23092..23634
                     /locus_tag="ACHJWA_002866"
     CDS             23092..23634
                     /locus_tag="ACHJWA_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFO4309426.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCINYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23639..24211
                     /gene="tsaC"
                     /locus_tag="ACHJWA_002867"
     CDS             23639..24211
                     /gene="tsaC"
                     /locus_tag="ACHJWA_002867"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFO4309427.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24216..25034
                     /gene="aroE"
                     /locus_tag="ACHJWA_002868"
     CDS             24216..25034
                     /gene="aroE"
                     /locus_tag="ACHJWA_002868"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFO4309428.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFV
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKPTADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            25031..25288
                     /locus_tag="ACHJWA_002869"
     CDS             25031..25288
                     /locus_tag="ACHJWA_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFO4309429.1"
                     /translation="MNQAIQFPDREEWVENKKCVCFPALVNGMQLTCAISGDSLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25264..25818)
                     /locus_tag="ACHJWA_002870"
     CDS             complement(25264..25818)
                     /locus_tag="ACHJWA_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFO4309430.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6689 a         6177 c         6762 g         6440 t
ORIGIN      
        1 aaagttctgg gctaattaca cgttaattag ttttgaattg aaaagggcgc ttcggcgccc
       61 tttttgctgc ccgtaatttg agctcgaaat atttagtcgt aattttgctc gctgaactat
      121 ggcgcaaata aaactgtgct attgtaatca taactattct catttacact ttgcgcagaa
      181 tttgaacggg agcaatcatg tacgtttgtt tgtgtaatgg tgtaagtgac aaaaaaattc
      241 gccaggcagt ccgtcagttt catccgcaat ctttccaaca attgcgtaag tttattcctg
      301 tgggaaatca atgtggtaag tgcattcgcg ccgcgcgcga agtgatgcag gacgagttga
      361 tgcagatgcc agaatttaaa gagattgcct gaagcccacg cttatttttt gacatcccgc
      421 tagccccatc tacgctctaa tagtggaagc ggagggacta taaaatgaaa ggtgatgtta
      481 aaatcataaa ttatctcaat aaactattgg gaaatgagct tgtcgcaatc aatcagtatt
      541 ttctccatgc ccggatgttt aaaaactggg gactgatgcg tcttaatgac gtcgaatacc
      601 atgaatccat cgatgagatg aaacacgctg ataaatatat cgagcgtatt ttgtttttag
      661 aaggaatccc caacttacag gatttaggta agctagggat cggtgaagat gtcgaagaga
      721 tgctgcaatc cgacctgcga ttagagctgg aaggtgcgaa ggatctgcgt gaggctatcg
      781 cgtatgccga cagcgttcat gactatgtta gccgcgatat gatgatcgag atccttgccg
      841 atgaagaagg tcatatcgac tggctcgaaa ctgaactgga tctcatcggt aaaatcggcc
      901 tacaaaatta cctgcaatca caaattaaag ttaatgatta acggcctatc agcaaactat
      961 cccagcctat aacgaaaccc gcagccgcca gaaatggccc aaaaggcagc gggtttttta
     1021 atgattcttt tcctctcatg agcagaccaa taactacggc tccgcaagcg aatgaggcag
     1081 caaggaaaac cagtcgtggt aagaaggccc atgaatgcca ggcgccaagg gcggcgagaa
     1141 acttcacatc accatagcct aatccttctt tatggcgcag tatgcgatac ccccagtaaa
     1201 tgacagcgaa tgtgccgtaa ccaataattg ccccccataa cgcatcggct aaaccatcag
     1261 gatgacaaac ttgatagaac aaaagacctg accagagtaa cgggcacgta aatctgtcag
     1321 gtaacaagcc atgctttgca tcccagaaaa aaagtagggc tgataaacaa gcgtacaaaa
     1381 ttagaaaggg aagggtagtg tccataaaac atcctctgta attggagtat caccatcgaa
     1441 taactccgga tgctcagcaa cagagagagc gctgatttgt taacctgatt ccggtgaaaa
     1501 acgctatctt cacaacctta caaatttcgt ggaatgcagc tcgcaatctg cgcgttgcga
     1561 ttaatttctg cccgatgctt gcgtcaggct cagatttgcg tataatgcgc gggcttgtcg
     1621 tagttgacag caggttcaat ctgaacctca gttagccgaa tttggctacc taacaatgct
     1681 cccaatcggg gagctacgta agaacggtta cactctccca tcaatcgtaa tgggtctgag
     1741 gagtaatcat tttcgtttat aaaataattg gagctctggt ctcatgcaga accaaagaat
     1801 ccgtatccgc ctgaaagcgt ttgatcatcg tctgatcgat caagcaaccg cggaaatcgt
     1861 cgagactgcc aagcgcactg gtgcgcaggt ccgtggtccg atcccgctgc cgacacgcaa
     1921 agagcgcttc actgttctga tctccccgca cgtcaacaaa gacgcgcgcg atcagtacga
     1981 aatccgtact cacttgcgtc tggttgacat cgttgagcca accgagaaaa ccgttgatgc
     2041 tctgatgcgt ctggatctgg ctgccggtgt agacgtgcag atcagcctgg gttaatcagg
     2101 tcattgagcg attgagaggt tgaaacaatg attggtttag tcggtaaaaa agtgggtatg
     2161 acccgtatct tcacagaaga cggcgtttct atcccagtaa ccgtaatcga agttgaagca
     2221 aaccgcgtta ctcaggttaa agacctggct aacgatggct accgtgctat tcaggtgacc
     2281 accggtgcta aaaaagctaa ccgtgtgacc aagcctgaag ctggccactt cgctaaagct
     2341 ggcgtagaag ctggccgtgg tctgtgggaa ttccgcctgg ctgaaggcga agagttcact
     2401 gtaggtcaga gcattagcgt tgaactgttt gctgacgtta aaaaagttga cgtaactggc
     2461 acctctaaag gtaaaggttt cgcaggtacc gttaagcgct ggaacttccg tacccaagac
     2521 gctactcacg gtaactcctt gtctcaccgc gttccgggtt ctatcggtca gaaccagact
     2581 ccgggcaaag tgttcaaagg caagaaaatg gcaggtcaga tgggtaacga acgtgtaacc
     2641 gttcagagcc ttgacgtagt acgcgttgac gctgagcgca acctgctgct ggttaaaggt
     2701 gctgtcccgg gtgcaaccgg tagcgacctg atcgttaaac cagctgtgaa ggcgtaagga
     2761 gatagcaatg gaattagtat tgaaagacgc gcagagcgcg ctgactgttt ccgaaactac
     2821 cttcggtcgt gatttcaacg aagcgctggt tcaccaggtt gttgttgctt atgcagctgg
     2881 tgctcgtcag ggtactcgtg ctcagaagac tcgtgctgaa gtaactggtt ccggtaaaaa
     2941 accgtggcgc cagaaaggca ccggccgtgc gcgttctggt tctatcaaga gcccgatctg
     3001 gcgttctggt ggcgtgacct ttgctgctcg tccgcaggac cacagtcaaa aagttaacaa
     3061 gaagatgtac cgcggcgcgc tgaaaagcat cctgtccgaa ctggtacgtc aggatcgtct
     3121 gatcgttgtc gagaagttct ctgtagaagc gccgaaaact aagctgctgg cacagaaact
     3181 gaaagacatg gctctggaag atgtgctgat catcaccggt gagctggacg aaaacctgtt
     3241 cctggctgcg cgcaacctgc acaaggttga cgtacgcgat gcaactggta tcgacccggt
     3301 tagcctgatc gccttcgaca aagtcgtaat gactgctgat gctgttaagc aagttgagga
     3361 gatgctggca tgattcgtga agaacgtctg ctgaaggtgc tgcgtgcacc gcacgtttct
     3421 gaaaaagcgt ctactgcgat ggaaaaatcc aacaccatcg tactcaaagt tgctaaagac
     3481 gcgaccaaag cagaaatcaa agctgctgtg cagaaactgt ttgaagtcga agtcgaagtc
     3541 gttaacaccc tggtagttaa agggaaagtt aaacgtcacg gacagcgtat cggtcgtcgt
     3601 agcgactgga aaaaagctta cgtcaccctg aaagaaggcc agaatctgga cttcgttggc
     3661 ggcgctgagt aagtcggagg agtaatacaa tggcagttgt taaatgtaaa ccgacatctc
     3721 cgggtcgtcg ccacgtagtt aaagtggtta accctgagct gcacaagggc aaaccttttg
     3781 ctccgttgct ggaaaaaaac agcaaatccg gtggtcgtaa caacaatggc cgtatcacca
     3841 ctcgtcatat cggtggtggc cacaagcagg cttaccgtat tgttgacttc aaacgcaaca
     3901 aagacggtat cccggcagtt gttgaacgtc ttgagtacga tccgaaccgt tccgcgaaca
     3961 tcgcgctggt tctgtacaaa gacggtgaac gccgttacat cctggcccct aaaggcctga
     4021 aagctggcga ccagattcag tctggcgttg atgctgcaat caaaccaggc aacaccctgc
     4081 cgatgcgcaa catcccggtt ggttctactg ttcataacgt agaaatgaaa ccaggtaaag
     4141 gcggtcagct ggcacgttcc gctggtactt acgttcagat cgttgctcgc gacggtgctt
     4201 atgtcaccct gcgtctgcgt tctggtgaaa tgcgtaaagt cgaagcagac tgccgtgcaa
     4261 ctctgggcga agttggcaat gctgagcata tgctgcgcgt tctgggtaaa gcaggtgctg
     4321 cacgctggcg tggtgttcgt ccgaccgttc gcggtaccgc gatgaacccg gtagaccacc
     4381 cacatggtgg tggtgaaggt cgtaactttg gtaagcaccc ggtaactccg tggggcgttc
     4441 agaccaaagg taagaagacc cgcagcaaca agcgtactga taaattcatc gtacgtcgcc
     4501 gtagcaaata attttagagg ataagccatg ccacgttctc tcaagaaagg tccttttatt
     4561 gacctgcact tgctgaagaa ggtagagaaa gcggtggaaa gcggagacaa gaagcccctg
     4621 cgcacttggt cccgtcgttc aacgatcttt cctaacatga tcggtttgac catcgctgtc
     4681 cataatggtc gtcagcacgt tccggtattt gtaaccgacg aaatggtcgg tcacaaactg
     4741 ggtgaattcg caccgactcg tacttatcgc ggccacgctg ctgataaaaa agcgaagaag
     4801 aaataaggta ggaggaagag atggaaacta tcgctaaaca tcgccatgct cgttcttctg
     4861 ctcagaaggt tcgccttgtt gctgacctga ttcgcggtaa gaaagtgtcg caggctctgg
     4921 atattttgac ctacaccaac aagaaagcgg ctgtactggt caagaaagtt ctggaatctg
     4981 ccattgctaa cgctgaacac aacgatggcg ctgacattga cgatctgaaa gttacgaaaa
     5041 ttttcgtaga cgaaggcccg agcatgaagc gcattatgcc gcgtgcaaaa ggtcgtgcag
     5101 atcgcatcct gaagcgcacc agccacatca ctgtggttgt gtccgatcgc tgagactctg
     5161 gagactagca atgggtcaga aagtacatcc taatggtatt cgcctgggta ttgtaaaacc
     5221 atggaactct acctggtttg cgaacaccaa agaattcgct gacaacctgg acagcgattt
     5281 taaagtacgt cagtacctga ctaaggaact ggctaaagcg tccgtatctc gtatcgttat
     5341 cgagcgtccg gctaagagca tccgtgtaac cattcacact gctcgcccgg gtatcgttat
     5401 cggtaaaaaa ggtgaagacg tagaaaaact gcgtaaggtc gtagcggaca tcgctggcgt
     5461 tcctgcacag atcaacatcg ccgaagttcg taagcctgaa ctggacgcaa aactggttgc
     5521 tgacagcatc acttctcagc tggaacgtcg cgttatgttc cgtcgtgcta tgaagcgtgc
     5581 tgtacagaac gcaatgcgtc tgggcgctaa aggtattaaa gttgaagtta gcggccgtct
     5641 gggcggcgcg gaaatcgcac gtaccgaatg gtaccgcgaa ggtcgcgtac cgctgcacac
     5701 tctgcgtgct gacatcgact acaacacctc tgaagcgcac accacttacg gtgtaatcgg
     5761 cgttaaagtg tggatcttca aaggcgagat cctgggtggt atggctgctg ttgaacaacc
     5821 ggaaaaaccg gctgctcagc ctaaaaagca gcagcgtaaa ggccgtaaat aaggagcgtc
     5881 gctgatgtta caaccaaagc gtacaaaatt ccgtaaaatg cataaaggcc gtaaccgcgg
     5941 tctggcgcag ggtacggatg ttagcttcgg cagcttcggt ctgaaagctg ttggccgtgg
     6001 tcgtctgact gcccgtcaga tcgaagcagc acgtcgtgct atgacccgtg cagttaagcg
     6061 tcaaggtaag atctggatcc gtgtgttccc ggacaaaccg atcactgaaa agccgctggc
     6121 agtgcgtatg ggtaaaggta aaggtaacgt ggagtattgg gttgccttga ttcagccggg
     6181 taaagtcctg tatgaaatgg acggtgttcc ggaagagctg gcccgtgaag cattcaagct
     6241 ggcagcagcg aaactgccga ttaaaaccac ctttgtaact aagacggtga tgtaatgaaa
     6301 gcaaaagagc tgcgtgagaa gagcgttgaa gagctgaaca ccgagctgct gaacctgctg
     6361 cgtgagcagt tcaacctgcg tatgcaggct gcaagtggcc agctgcaaca gtctcacctg
     6421 ttgaagcaag tgcgtcgcga tgtcgcacgc gttaagactt tactgaacga gaaggcgggt
     6481 gcgtaatgac cgataaaatc cgtactctgc aaggtcgcgt tgttagcgac aaaatggaga
     6541 aatccattgt tgttgctatc gaacgttttg tgaaacaccc gatctacggt aaattcatca
     6601 agcgtacgac caaactgcac gtacatgacg agaacaacga atgcggtatc ggtgacgtgg
     6661 ttgaaatccg cgaatgccgt ccgctgtcca agactaaatc ctggacgctg gttcgcgttg
     6721 tagagaaagc ggttctgtaa tacagtacac tctctcgata cgaataaacg gctcagaaat
     6781 gagccgttta ttttttctac ccatatcctt gaagcggtgt tataatgccg cgccctcgat
     6841 atggggattt ttaacgacct gattttcggg tctcagtagt agttgacatt agcggagcac
     6901 taaaatgatc caagaacaga ctatgctgaa cgtcgccgac aactccggtg cacgtcgcgt
     6961 aatgtgtatc aaggttctgg gtggctcgca ccgtcgctac gcaggcgtag gcgacatcat
     7021 caagatcacc atcaaagaag caattccgcg tggtaaggtc aaaaaaggtg atgtgctgaa
     7081 ggcggtagtg gtgcgcacca agaagggtgt tcgtcgcccg gacggttctg tcattcgctt
     7141 cgatggtaat gcttgtgttc ttctgaacaa caacagcgag cagcctatcg gtacgcgtat
     7201 ttttgggccg gtaactcgtg agcttcgtag tgagaagttc atgaaaatta tctctctggc
     7261 accagaagta ctctaaggag cgaatcatgg cagcgaaaat ccgtcgtgat gacgaagtta
     7321 tcgtgttaac cggtaaagat aaaggtaaac gcggtaaagt taagaatgtc ctgtcttccg
     7381 gcaaggtcat tgttgaaggt atcaacctgg ttaagaaaca tcagaagccg gttccggccc
     7441 tgaaccaacc gggtggcatc gttgaaaaag aagccgctat tcaggtttcc aacgtagcaa
     7501 tcttcaatgc ggcaaccggc aaggctgacc gtgtaggctt tagattcgaa gacggcaaaa
     7561 aagtccgttt cttcaagtct aacagcgaaa ctatcaagta atttggagta gtacgatggc
     7621 gaaactgcat gattactaca aagacgaagt agttaaaaaa ctcatgactg agtttaacta
     7681 caattctgtc atgcaagtcc ctcgggtcga gaagatcacc ctgaacatgg gtgttggtga
     7741 agcgatcgct gacaaaaaac tgctggataa cgcagcagca gacctggcag caatctccgg
     7801 tcaaaaaccg ctgatcacca aagcacgcaa atctgttgca ggcttcaaaa tccgtcaggg
     7861 ctatccgatc ggctgtaaag taactctgcg tggcgaacgc atgtgggagt tctttgagcg
     7921 cctgatcact attgctgtac ctcgtatccg tgacttccgt ggcctgtccg ctaagtcttt
     7981 cgacggtcgt ggtaactaca gcatgggtgt ccgtgagcag atcatcttcc cagaaatcga
     8041 ctacgataaa gtcgaccgcg ttcgtggttt ggatattacc attaccacta ctgcgaaatc
     8101 tgacgaagaa ggccgcgctc tgctggctgc ctttgacttc ccgttccgca agtaaggtag
     8161 ggttactaaa tggctaagca atcaatgaaa gcacgcgaag taaaacgcgt agctttagct
     8221 gataaatact tcgcgaaacg cgctgaactg aaagcgatca tctctgatgt gaacgcttcc
     8281 gacgaagatc gttggaacgc tgttctcaag ctgcagactc tgccgcgtga ttccagcccg
     8341 tctcgtcagc gtaaccgctg ccgtcaaaca ggtcgtccgc atggtttcct gcggaagttc
     8401 gggttgagcc gtattaaggt ccgtgaagcc gctatgcgcg gtgaaatccc gggtctgaaa
     8461 aaggctagct ggtaattgtc accaattgaa tcacgggagt aaagacagat gagcatgcaa
     8521 gatccgatcg cggatatgct gacccgtatc cgtaacggtc aggccgcgaa caaagctgcg
     8581 gtcaccatgc cttcctccaa gctgaaagtg gcaatcgcca acgtgctgaa ggaagaaggt
     8641 tttattgaag attttaaagt tgaaggcgac accaagcctg aactggaact tactctgaag
     8701 tatttccagg gcaaagctgt tgtagaaagc attcagcgtg tcagccgccc aggtctgcgc
     8761 atctataaac gtaaagatga gctgccgaaa gttatggcgg gtctgggtat cgcagttgtt
     8821 tctacctcta aaggtgttat gactgatcgt gcagcgcgcc aggctggtct tggtggcgaa
     8881 attatctgct acgtagccta atcggaggaa aaaatgtctc gtgttgctaa agcaccggtc
     8941 gttgttcctg ccggcgttga cgtaaaaatc aacggtcagg ttattacgat caaaggtaaa
     9001 aacggcgagc tgactcgtac tctcaacgat gctgttgaag ttaaacatgc agataatacc
     9061 ctgaccttcg gtccgcgtga tggttacgca gacggttggg cacaggctgg taccgcgcgt
     9121 gccctgctga actcaatggt tatcggtgtt accgaaggct tcactaagaa gctgcagctg
     9181 gttggtgtag gttaccgtgc agcggttaaa ggcaatgtga ttaacctgtc tctgggtttc
     9241 tctcatcctg ttgaccatca gctgcctgcg ggtatcactg ctgaatgtcc gactcagact
     9301 gaaatcgtgc tgaaaggcgc tgataagcag gtgatcggcc aggttgcagc ggatctgcgc
     9361 gcctaccgtc gtcctgagcc ttataaaggc aagggtgttc gttacgccga cgaagtcgtg
     9421 cgtaccaaag aggctaagaa gaagtaaggt aacactatgg ataagaaatc tgctcgtatc
     9481 cgtcgtgcga cccgcgcacg ccgcaagctc caggagctgg gcgcaactcg cctggtggta
     9541 catcgtaccc cgcgtcacat ttacgcacag gtaattgcac cgaacggttc tgaagttctg
     9601 gtagctgctt ctactgtaga aaaagctatc gctgaacaac tgaagtacac cggtaacaaa
     9661 gacgcggctg cagctgtggg taaagctgtc gctgaacgcg ctctggaaaa aggcatcaaa
     9721 gatgtatcct ttgaccgttc cgggttccaa tatcatggtc gtgtccaggc actggcagat
     9781 gctgcccgtg aagctggcct tcagttctaa ggtagaggtg taagatggct cacatcgaaa
     9841 aacaagctgg cgaactgcag gaaaagctga tcgcggtaaa ccgcgtatct aaaaccgtta
     9901 aaggtggtcg tattttctcc ttcacagctc tgactgtagt tggcgatggt aacggtcgcg
     9961 ttggttttgg ttacggtaaa gcgcgtgaag ttccagcagc gatccagaaa gcgatggaaa
    10021 aagcccgtcg caatatgatt aacgtcgcgc tgaataacgg cactctgcaa caccctgtta
    10081 aaggtgttca cacgggttct cgcgtattca tgcagccggc ttctgaaggt accggtatca
    10141 tcgccggtgg tgcaatgcgc gccgttctgg aagtcgctgg ggttcataac gttctggcta
    10201 aagcatatgg ttccaccaac ccgatcaacg tggttcgtgc aactattgat ggcctggaaa
    10261 atatgaattc tccagaaatg gtcgctgcca agcgtggtaa atccgttgaa gaaattctgg
    10321 ggaaataaac catggcaaag actattaaaa ttactcaaac ccgcagtgca atcggtcgtc
    10381 tgccgaaaca caaggcaacg ctgcttggcc tgggtctgcg tcgtattggt cacaccgtag
    10441 agcgcgagga tactcctgct attcgcggta tgatcaacgc ggtttccttc atggttaaag
    10501 ttgaggagta agagatgcgt ttaaatactc tgtctccggc cgaaggctcc aaaaaggcgg
    10561 gtaaacgcct gggtcgtggt atcggttctg gcctcggtaa aaccggtggt cgtggtcaca
    10621 aaggtcagaa gtctcgttct ggcggtggcg tacgtcgcgg tttcgagggt ggtcagatgc
    10681 ctctgtaccg tcgtctgccg aaattcggct tcacttctcg taaagcagcg attacagccg
    10741 aagttcgtct gtctgacctg gctaaagtag aaggcggtgt agtagacctg aacacgctga
    10801 aagcggctaa cattatcggt atccagatcg agttcgcgaa agtgatcctg gctggcgaag
    10861 taacgactcc ggtaactgtt cgtggcctgc gtgttactaa aggcgctcgt gctgctatcg
    10921 aagctgctgg cggtaaaatc gaggaataag tagcagatgg ctaaacaacc gggattagat
    10981 tttcaaagtg ccaaaggtgg cttaggcgag ctgaaacgca gactgctgtt tgttatcggt
    11041 gcgctgattg tgtttcgtat tggctctttt attccgatcc ctggtattga tgccgctgta
    11101 cttgccaaac tgcttgagca acagcgaggc accatcattg agatgtttaa catgttctct
    11161 ggtggtgctc tcagccgtgc ttctatcttt gctctgggga tcatgccgta tatttcggcg
    11221 tcgatcatta tccagctgct gacggtggtt cacccaacgt tggcagaaat taagaaagaa
    11281 ggggagtctg gtcgtcgtaa gatcagccag tacacccgtt acggtactct ggtgctggca
    11341 atattccagt cgatcggtat tgctaccggt ctgccgaata tgcctggtat gcaaggcctg
    11401 gtgattaacc cgggctttgc attctacttc accgctgttg taagtctggt cacaggaacg
    11461 atgttcctga tgtggttggg cgaacagatc actgaacgag gtatcggcaa cggtatttca
    11521 atcattatct tcgccggtat tgtcgcggga ctcccgccag ccattgccca tactatcgag
    11581 caagcgcgtc aaggcgacct gcacttcctc gtgttgctgt tggttgcagt attagtattt
    11641 gcagtgacgt tctttgttgt atttgttgag cgtggtcaac gccgcattgt ggtaaactac
    11701 gcgaaacgtc agcaaggtcg tcgtgtctat gctgcacaga gcacacattt accgctgaaa
    11761 gtgaatatgg cgggggtaat cccggcaatc ttcgcttcca gtattattct gttcccggcg
    11821 accatcgcgt catggttcgg gggcggtact ggttggaact ggctgacaac aatttcgctg
    11881 tatttgcagc ctgggcaacc gctttatgtg ttactctatg cgtctgcaat catcttcttc
    11941 tgtttcttct acacggcgtt ggttttcaac ccgcgtgaaa cagcagataa cctgaagaag
    12001 tccggtgcat ttgtaccagg aattcgtccg ggagagcaaa cggcgaagta tatcgataaa
    12061 gtaatgaccc gcctgaccct ggttggtgcg ctgtatatta cctttatctg cctgatcccg
    12121 gagttcatgc gtgatgcaat gaaagtaccg ttctacttcg gtgggacctc actgcttatc
    12181 gttgttgtcg tgattatgga ctttatggct caagtgcaaa ctctgatgat gtccagtcag
    12241 tatgagtctg cattgaagaa ggcgaacctg aaaggctacg gccgataatt ggtcgcccga
    12301 gaagttacgg agagtaaaaa tgaaagttcg tgcttccgtc aagaaattat gccgtaactg
    12361 caaaatcgtt aagcgtgatg gtgtcatccg tgtgatttgc agtgccgagc cgaagcataa
    12421 acagcgccaa ggctgatttt ttcgcatatt tttcttgcaa agttgggttg agctggctag
    12481 attagccagc caatcttttg tatgtctgtg cgtttccatt tgagtatcct gaaaacgggc
    12541 ttttcagcat ggaacgtaca tattaaatag taggagtgca tagtggcccg tatagcaggc
    12601 attaacattc ctgatcataa gcatgccgta atcgcattaa cttcgattta tggcgtcggc
    12661 aagacccgtt ctaaagccat cctggctgca gcgggtatcg ctgaagatgt taagatcagt
    12721 gagctgtctg aaggacaaat cgacacgctg cgtgacgaag ttgccaaatt tgtcgttgaa
    12781 ggtgatctgc gccgtgaaat cagcatgagc atcaagcgcc tgatggatct tggttgctat
    12841 cgcggtttgc gtcatcgtcg tggtctcccg gttcgcggtc agcgtaccaa gaccaacgca
    12901 cgtacccgta agggtccgcg caaaccgatc aagaaataat cggggtgatt gaataatggc
    12961 aaaggcacca attcgtgcac gtaaacgtgt aagaaaacaa gtctctgacg gcgtggctca
    13021 tatccatgct tctttcaaca acaccatcgt gactatcact gatcgtcagg gtaacgcgtt
    13081 gggttgggca acagccggtg gttccggttt ccgtggttct cgcaaatcca ctccgtttgc
    13141 agctcaggtt gcagcagagc gttgcgctga cgccgtgaaa gaatacggca tcaagaatct
    13201 ggaagttatg gttaaaggtc cgggtccagg ccgcgaatct actattcgtg ctctgaacgc
    13261 cgcaggtttc cgcatcacta acattactga tgtgactccg atccctcata acggttgtcg
    13321 tccgccgaaa aaacgccgcg tataacgcct cgttttccag gtttgttgga gaaagaaaat
    13381 ggcaagatat ttgggtccta agctcaagct gagccgtcgt gagggcaccg acttattcct
    13441 taagtctggc gttcgcgcga tcgataccaa gtgtaaaatt gaacaagctc ctggccagca
    13501 cggtgcgcgt aaaccgcgtc tgtctgacta tggtgtgcag ttgcgtgaaa agcaaaaagt
    13561 tcgccgtatc tatggtgtgc tggagcgtca gttccgtaac tactacaaag aagcagcacg
    13621 tctgaaaggc aacaccggtg aaaacctgtt ggctctgctg gaaggtcgtc tggacaacgt
    13681 tgtataccgt atgggcttcg gtgccactcg tgcagaagca cgtcagctgg ttagccataa
    13741 agcaattatg gtaaacggtc gtgttgttaa catcgcttct tatcaggtta gtccgaatga
    13801 cgttgtaagc attcgtgaga aagcgaagaa gcagtctcgc gtgaaagccg ctctggagct
    13861 ggctgagcag cgtgaaaagc caacctggct ggaagttgat gctggcaaga tggaaggtac
    13921 gtttaagcgt aagccggagc gttctgatct gtctgcggac attaacgaac acctgatcgt
    13981 cgagctttac tccaagtaaa gcttagtacc aaagagagga cacaatgcag ggttctgtga
    14041 cagagtttct aaaaccgcgc ctggttgata tcgagcaagt gagttcgacg cacgccaagg
    14101 tgacccttga gcctttagag cgtggctttg gccatactct gggtaacgca ctgcgccgta
    14161 ttctgctctc atcgatgccg ggttgcgcgg tgaccgaggt tgagattgat ggtgtactac
    14221 atgagtacag caccaaagaa ggcgttcagg aagatatcct ggaaatcctg ctcaacctga
    14281 aagggctggc ggtgagagtt cagggcaaag atgaagttat tcttaccttg aataaatctg
    14341 gcattggccc tgtgactgca gccgatatca cccacgacgg tgatgtcgaa atcgtcaagc
    14401 cgcagcacgt gatctgccac ctgaccgatg agaacgcgtc tattagcatg cgtatcaaag
    14461 ttcagcgcgg tcgtggttat gtgccggctt ctacccgaat tcattcggaa gaagatgagc
    14521 gcccaatcgg ccgtctgctg gtcgacgcat gctacagccc tgtggagcgt attgcctaca
    14581 atgttgaagc agcgcgtgta gaacagcgta ccgacctgga caagctggtc atcgaaatgg
    14641 aaaccaacgg cacaatcgat cctgaagagg cgattcgtcg tgcggcaacc attctggctg
    14701 aacaactgga agctttcgtt gacttacgtg atgtacgtca gcctgaagtg aaagaagaga
    14761 aaccagagtt cgatccgatc ctgctgcgcc ctgttgacga tctggaattg actgtccgct
    14821 ctgctaactg ccttaaagca gaagctatcc actatatcgg tgatctggta cagcgtaccg
    14881 aggttgagct ccttaaaacg cctaaccttg gtaaaaaatc tcttactgag attaaagacg
    14941 tgctggcttc ccgtggactg tctctgggca tgcgcctgga aaactggcca ccggcaagca
    15001 tcgctgacga gtaaccggat cacaggttaa ggttttactg agaaggataa ggtcatgcgc
    15061 catcgtaaga gtggtcgtca actgaaccgc aacagcagcc atcgccaggc tatgttccgc
    15121 aatatggcag gttcactggt tcgtcatgaa atcatcaaga cgactctgcc taaagcgaaa
    15181 gagctgcgcc gcgtagttga gccgctgatt actcttgcca agactgatag cgttgctaat
    15241 cgtcgtctgg cattcgcccg tactcgtgat aacgagatcg tggcaaaact gtttaacgaa
    15301 ctgggcccgc gtttcgcgag ccgtgccggt ggttacactc gtattctgaa gtgtggcttc
    15361 cgtgcaggcg acaacgcgcc gatggcttac atcgagctgg ttgatcgttc agagaaagca
    15421 gaagctgctg cagagtaatc tgaagcaacg taaaaaaacc cgccccggcg ggttttttta
    15481 tacccgtagc atccccactt atctacaata gctgtactct ttttgttcat cccctggagt
    15541 atttatgtgg ttacttgacc agtgggcaga gcgccatata gcagaagcgc aagcgaaagg
    15601 tgagtttgat aacctggcag gtagcggcga accattgata ctggatgatg attctcatgt
    15661 gccaccggaa ttacgtgcgg ggtatcgctt gctgaagaat gccggttgct taccgccaga
    15721 acttgagcaa cggagggaag caatccagct tctggatatc ctcaaaggta tccgtcacga
    15781 tgatccgcaa tatcaagagg ttagccgtcg attgtcatta ctggaattga agctgcgaca
    15841 agctggattg agtaccgatt ttttacgcgg cgattatgct gacaagttgt tgaataaaat
    15901 caacgataac tagtggagta tgtatgtatc gcattggtga gctggcaaaa atggcggaag
    15961 taacacccga cacgattcgt tattacgaaa agcagcagat gatggagcat gaagtgcgta
    16021 ccgaaggtgg gtttcgcctg tataccgaaa gcgatctcca gcgattgaaa tttatccgcc
    16081 atgccagaca actaggtttc agtctggagt cgatccgcga gttgctgtcg atccgtatcg
    16141 atcctgaaca ccatacctgt caggagtcaa aaggcattgt gcaggaaaga ttgcaggaag
    16201 tcgaagcacg gatagccgag ttgcagagta tgcagcgttc cttgcaacgc cttaacgatg
    16261 cctgttgtgg gaccgcccat agcagtgttt attgctcgat tcttgaagct cttgaacaag
    16321 gggcgagtgg cgttaagagt ggctgttgat tttttgcact ggcaggatta cactcacgcc
    16381 gttaaataac caactggagt ttttatgagc cgatatcagc atactaaagg gcagataaag
    16441 gataatgcga tagaagcatt actacatgat cccttattcc gacagcgcgt agagaaaaat
    16501 aagaagggga aaggcagtta catgcgaaaa ggaaaacatg gcaatcgggg gaactgggag
    16561 gccagtggca agaaagtaaa tcactttttt accactggtc ttctgctttc aggggcttgt
    16621 taagagcggt tattctgctc tttcagcaaa tcacgaattt ctgtcagtaa tacttcttct
    16681 ttagttggtg ctggtgcggc tgctggttct tcttttttcc gattcagttt gttgattagc
    16741 ttaatcgcca taaagatggc aaaggccaca atcagaaaat caaagacgtt ttgaatgaag
    16801 acaccgtaat gcatcacaac agcagggata tccccctgcg catcgcgtag cgtgacagca
    16861 aactgtttaa agtcgatccc gccaattaat aagcccagtg gtggcatgat gatatcggca
    16921 accagtgaag atacaatctt cccgaatgcc gcaccgataa tgacacccac cgccaaatcc
    16981 accacgttcc cgcgcatcgc aaattcgcga aattctttaa taatgctcat gttattctcc
    17041 ctatgaagcc gacaaccata agtctaacaa atgttaagcc attttcctgc caggaatgaa
    17101 tttaattaat ccttgataat caaagggaaa taataaggcg tcattagacg ccttattaat
    17161 tacaagaaga aagggcttgg ctggaagagt ctttcgacgt cggtaataaa ttttttatct
    17221 gtgaggaaca taattacgtg atcgccttgc tcaatgcgca gattgtcatt ggcaatcatc
    17281 acgtcgtttc cacgtaccac cgctccaata atcgttcctg gcggtagctt gatttcgtca
    17341 atgactctgc cgacaacgcg tgaggtgctt tcatcaccgt gagcaacggc ttcaatagct
    17401 tctgctacgc cgcggcgcaa tgaggaaaca ccaacaatat ctgcttttcg cacatggcta
    17461 agcaacgcag aaatagttgc ttgttgtggt gaaatcgcaa tatcgatcac gctcccctga
    17521 accagatcca cataagcgcg acgctggatc aataccatca cctttttcgc acccatacgt
    17581 ttggcaagca tggcggacat gatattggcc tcgtcatcgt tggtgacggc aataaacaga
    17641 tcaacttgat cgatatgttc ttcggccagc agttcttgat ccgacgcatc accaaaaaag
    17701 acgatcgtat tctgcaactt ttccgccagt tcggcagcgc gctgctgatt acgttcgatg
    17761 agtttaacgc tgtaatcttt ttccagacga cgcgccagcc ctgcaccgat attaccgcca
    17821 ccaaccagca tgatccgctt atacggtttt tccagtcgct gtaattcact catcaccgcg
    17881 cggatatgct gtgaagcggc aataaagaac acttcatcac cagcctcaac aatggtcgaa
    17941 ccttgcggac gaatggggcg atcgtggcgg aaaattgctg ccacacgagt atcgatatgt
    18001 ggcatatgtt cgcgcatggt cgaaagtgca ttaccaatca gcgggccgcc ataataggct
    18061 ttaaccacag cgaggctgac tttaccctca gcgaagttca ccacctgcaa tgcgccggga
    18121 tactcaatca gtcggtaaat attatcgata accaactgct ctggtgcgat cagatgatca
    18181 atcggtacag catctgaatg aaatagctta tcggcatcgc gtacgtagtc tggtgagcgg
    18241 atacgagcga tgcgattagg ggtgttgaaa agtgagtagg ctacctggca ggcaaccata
    18301 ttggtttcat ctgaactggt tacagcaacc agcatatcgg cgtcgtcggc acctgcctcc
    18361 cgcaatacgc gtggatgaga gccatgcccc tgcacgactc gcaggtcaaa tttatcctgc
    18421 aaggttcgca gacgctcacc gttggtatcg acaacagtga tatcgttgtt ctcgccaacc
    18481 aggttttccg ccagtgtgcc gccaacctgg ccggcaccca gaatgataat tttcatcagt
    18541 cgcgacccgt tatctcatca ctttttgatt agcttagcgt aaaagaagcc gtcgccctct
    18601 tcggcaccag gcagattttg tttacccggt tgctctggtg ttcctgtttc gcaaagttcg
    18661 gcatcagcgg tacgttgcaa aaaggcttta atctgcaggc tattctcttc cggtaacacc
    18721 gaacaggtgg catagaccag ggttccaccg gattttaaat gcggccaaat ggcgtcgaga
    18781 atttcagact gcaattgcgc gagttctggg atatcgcgat cgcgacgtaa ccatttaata
    18841 tccggatggc gacgaatcac accggttgct gaacaaggcg catctaataa aatgcgatca
    18901 aactgttgct cgccacacca ttgggaaggg taacggccat caccttgttt cacggttgcc
    18961 ttcataccaa ggcgttttaa attgtcgtaa acgcgagaga ggcgctgttc gtcaatatca
    19021 accgcaacaa cctgcgcttc tggtgccact tcaaggatat gcgttgtttt accgccgggg
    19081 gcggcacaaa gatccagaat gtgttcaccg ttttgtggcg caagccaggt catgcaacct
    19141 tgtgctgatg catcctgaac ggtaacccat ccgtcttcaa aaccaggtag cgcatgaaca
    19201 ggtgcaggtg tttccagccg tacagcatca gggtaatccg catgcgggaa acctttcatt
    19261 cctgcttcat ccagcaatgc aagccaggtg tcgcgggaat gatgcgtacg attaatacgc
    19321 agccacattg gcggacgctg gttattggct tcgacgatgg attgccactg ctctggatac
    19381 gctttttgca gacgcttcag caaccaggaa ggatgcagat aacgtgcatc actggtatta
    19441 aactcggcta ataactcttc ttgctgacgc tggaactggc gtaatacacc gttaatcaac
    19501 cctttaagtt gcggacgctt aattgcgata gcgccttcaa ccgtttcagc cagcgcagca
    19561 tgaggtggaa tgcgggtata aagcagttga tacaaaccaa ccataatcag gtaatgcacg
    19621 gtccgctgtt tgccggtcat cggacgggcc attgacttat taatcagcca gtctaactgc
    19681 gaaagcgtac gcagtacgcc aaagcacaac tcttgaagaa gtgctttgtc tttatcggaa
    19741 actttttgct ggagcggtgg cagaatgttg cttaatgatt gcccttgctc gacgacttgt
    19801 tcaacggcct gggccgccat gctacgtaaa ttacgttgtt ttttcataag tataaaaatg
    19861 cccggcaaga ccgggcttag aagagtggac tatcagacca gacggttgcc cggaacaaac
    19921 cattcccgac gagagttcag gaggtcttgc gcgctcatcg ctttcttacc cgcaggttgt
    19981 aacgagagca ggttcaggat gccatcacca gtcgcaacct gaatgccttg tttgttggct
    20041 tcaaggatcg ttcctggtgc agcgttggtt gccgtatcaa tgaccgatgc tttccagact
    20101 ttaaccggct gtccttcaat ttccacccag ctcattggcc atggattgaa agcgcgaatg
    20161 cagcgttcaa gctgtgctgc cgaaagtgac cagtcaatac gcgcttcttc tttactcaac
    20221 ttctcggcgt aagtgacaag agtttcgtcc tgaacttctg gtttcgccgt gccgtctgcc
    20281 aattgtttca acgtggtgat aagcccttgt gggccaagct ctgccagctt gtcgtacagc
    20341 gtaccactgg tatcttctgc agtaatcggg caggagagct tatagagcat gtcaccggtg
    20401 tctaaaccaa catccatttg cataatggtc acaccagttt ctgcatcacc cgcccatagt
    20461 gagcgttgga ttggtgcagc accgcgccag cgtggcagca gtgaaccatg aacgttgata
    20521 cagccaagac gcggcatctc cagcactgct ttaggcagaa ttaagccata ggcgacgaca
    20581 accataacat cagcctgtag atcggcgacc agttgctggt tttcttgtgg acgcagggaa
    20641 acaggttgaa aaacgggcag acctttttcc tcagccagaa ctttaaccgg gctgggcatc
    20701 agttttttac cgcgtcctgc cggtcggtct ggctgggtga acacgccaac gacgttatga
    20761 ccagaagaca acagcgcgtc gagatgacgc gctgcaaagt caggtgtacc cgcaaaaata
    20821 atacgtagtg attctgacac gttagttctt atccttaagc ccgggctttc agacgatcca
    20881 gtttttcaac tttctgacga atacgttgtt gtttcagcgg tgacagataa tccataaaca
    20941 gtttgccgac caggtgatcc atctcatgct gaatacagat ggctaacaga ccatctgcct
    21001 ccagttcaaa tggtttaccg tcgcggtcca gggcgcgaat tttaactttc tctgcgcgtg
    21061 gcactaaagc acgttgttca gggatcgaca ggcaaccttc ttcaatgcct gtttcgccgc
    21121 ttttttctaa aagctccgga ttgattaaca ccagccgttc gtcacggttt tccgaaacat
    21181 caataacaat gatacgttga tggatatcaa cctgggttgc cgccaggcca ataccttctt
    21241 ctgcgtacat cgtctcgaac atatcatcga cgatacgctg aatttctgca ttcacttctt
    21301 ctaccggttt agcaactttg cgaagccgct cgtccggaat atgtaacact tgcaaaactg
    21361 acataaatct ccagagatgt gttcaggagt tagaaagatt atttcttcta ttctagacaa
    21421 atccccctct gattgacagc atcactgacc aatcgcaaag attgctaagg ctgcttatgg
    21481 cagggagata aggatggtcg atacagaaat ttggctgcgt ttaatgagta tcagcagctt
    21541 gtacggcgat gatatggtcc gtatagctca ctggctggca agacagtcgc atattgatgc
    21601 ggttgtattg cagcaaacag ggcttacatt gcggcaggca caacgctttc tttcatttcc
    21661 gcggaagagt atcgaaagct cactttgttg gttggagcaa cccaaccatc atttaatccc
    21721 tgcggacagc gaattttatc ctcctcaact tctggcgacg acagattacc ccggtgcact
    21781 gtttgttgaa ggagaactgc acgcgctgca ttcatttcag cttgccgtag tggggagtcg
    21841 ggcgcattca tggtatggcg agcgatgggg acgattattt tgcgaaactc tggcgaagca
    21901 tggagtgaca attacgagtg gactggcgcg tggaatcgat ggtgtagcgc ataaagcagc
    21961 cttacaggta aatggcgtca gcattgctgt attggggaat ggacttaata ccattcatcc
    22021 ccgccgtcat gcccgactgg ctgccagtct gcttgaacag gggggcgctc tcgtctcgga
    22081 atttcccctc gatgttccac cccttgctta caatttccca cgaagaaatc gcattatcag
    22141 tggtctaagt aaaggtgtac tggtggtgga agcggctttg cgtagtggtt cgctggtgac
    22201 agcacgttgt gcgcttgagc aggggcgaga agtttttgcc ttgccaggtc caatagggaa
    22261 tccgggaagc gaagggcctc actggttaat aaaacaaggt gcgattcttg tgacggaacc
    22321 ggaagaaatt ctggaaaact tgcaatttgg attgcactgg ttgccagacg cccctgaaaa
    22381 ttcattttat tcaccagatc aggaagacgt ggcattgcca tttcctgagc tcctggctaa
    22441 cgtaggagat gaggtaacac ctgttgacgt cgtcgctgaa cgtgccggcc aacctgtgcc
    22501 agaggtagtt actcaactac tcgaactgga gttagcagga tggatcgcag ctgtacccgg
    22561 cggctatgtc cgattgagga gggcatgcca tgttcgacgt actaatgtat ttgtttgaaa
    22621 cctatattca cacagaagct gagttgcgtg tggatcaaga caaacttgaa caggatctta
    22681 ccgacgcagg atttgatcga gaagatatct acaatgccct gctttggctg gaaaagcttg
    22741 cggattatca ggaaggactg gcagaaccga tgcaactggc ctctgatcca ctctccatgc
    22801 gtatttatac ccctgaggag tgtgaaagac tggatgccag ctgtcgtggt tttctgcttt
    22861 tccttgagca aattcaggtg cttaaccttg aaacccgtga aatggtgata gagcgagtgc
    22921 tcgcgctgga taccgcagag ttcgacctgg aagacctgaa atgggtaatc ctgatggtgt
    22981 tgttcaatat tccgggctgc gaaaatgcgt accagcaaat ggaagaatta ctctttgaag
    23041 tgaatgaagg tatgctgcat taatcatttc tttaattcag cataagttgt tatggcgaaa
    23101 tcagcactgt tcacggtgcg taataatgag tcctgcccaa agtgcggggc tgaactggtt
    23161 attcgatccg ggaaacacgg tccgtttctt ggatgctcac agtatccggc gtgtgactac
    23221 gtccgtcctc tgaaatcttc agcggatgga catatcgtca aagttctgga ggggcaggtt
    23281 tgccctgcat gtggcgcaaa tctggtatta cgccagggac gctttggtat gtttattggt
    23341 tgcattaact accctgaatg cgaacatacc gaacttatcg ataaaccgga cgaaacagca
    23401 attacatgtc cccaatgccg gacgggccat ctggtccagc gccgctcccg ttatggcaaa
    23461 acatttcact cctgtgatcg ctacccggag tgtcaatttg ccattaactt caaacccata
    23521 gctggagaat gccctgagtg tcattatccg ctactcatcg aaaagaaaac cgcgcagggt
    23581 gtaaaacact tttgtgccag taaacaatgt ggaaagccgg tttcggcgga ataataacgt
    23641 gaataataac ctgcaaggag acgctatcgc agctgcgata gatgttctca atgaagaacg
    23701 tgtcatcgcc tatccaacgg aagccgtttt cggtgttggg tgcgatcctg atagcgaaac
    23761 agcagtgatg cgactgttgg agttaaaaca gcgtccggtt gataaggggc tgattttaat
    23821 cgcagcaaat tacgagcagc ttaaacccta tattgatgac accatgttga ctgacgtgca
    23881 gcgtgaaacc attttttccc gctggccagg tcctgtcacc tttgtctttc ccgcgcctgc
    23941 gacaacaccg cgctggttga cgggccgctt tgattcgctt gctgtacgag tcaccgacca
    24001 tccgttggtg gttgctttgt gccaggctta tggtaaaccg ctggtttcta ccagtgccaa
    24061 cttgagtgga ttgccacctt gtcgaacagt agacgaagtt cgcgcacaat ttggcgcggc
    24121 gttcccggtt gtgcctggtg aaacgggggg gcgtttaaat ccttcagaaa tccgcgatgc
    24181 cctgacgggt gaactgtttc gacaggggta acataatgga aacctatgct gtttttggta
    24241 atccgatagc ccacagcaaa tcgccattca ttcatcagca atttgctcag caactgaata
    24301 ttgaacatcc ctatgggcgc gtgttggcac ccatcaatga tttcgtcaac acactgaacg
    24361 ctttctttag tgctggtggt aaaggtgcga atgtgacggt gccttttaaa gaagaggctt
    24421 ttgccagagc ggatgagctt actgaacgcg cagcgttggc tggtgctgtt aacaccctca
    24481 agcggttaga agatggacgc ttgctgggtg acaataccga tggtgtaggc ttgttaagcg
    24541 atctggaacg tctgtctttt atccgtcctg gtttacgtat tctgctcatc ggcgcaggtg
    24601 gagcatctcg cggcgtacta ctgccacttc tttccctgga ctgtgcggtg acaatcacta
    24661 atcggacggt atcccgagcg gaagagttgg ctaaattgtt tgcgcacact ggcagtattc
    24721 aggcgttggg tatggacgaa ctggaaggtc atgagtttga tctcattatt aatgccacat
    24781 ccagtggcat cagtggtgat attccggcga tcccgtcatc gctcattcat ccaggcattt
    24841 attgctatga catgttctat cagaaaggaa aaactccttt tttggcatgg tgtgagcagc
    24901 gaggctcaaa gcctactgct gatggtttag gaatgctggt ggcacaggcg gctcatgcct
    24961 tccttctttg gcatggcgtt ctgcctgatg tagaaccagt tataaagcaa ttgcaggagg
    25021 aattgtccgc gtgaatcagg ccatccagtt tccggacaga gaagagtggg tcgagaataa
    25081 aaaatgtgta tgttttcccg ctctcgtgaa tggcatgcaa ctgacatgtg cgatctctgg
    25141 cgatagcctg gcgtatcgct ttacaggaga tacgccagag cagtggttag cgagttttcg
    25201 tcagcatcgc tgggacttgg aagaagaagc ggaaaactta attcaggaac aaagtgaaga
    25261 tgatcaaggc tgggtctggt taccctgatc cagatattcg tccttccatt tcacgtaatt
    25321 attcgcggaa tagcgtaacc cagccttctc ttcatcactt aacgggcgga tctgtttgac
    25381 gggactaccg agatacagat atccgctctc cagccgttta ttttgtggga ccagactacc
    25441 cgcaccaatc atcacatcat cttctactat tgcgccatca agtaaaattg agcccatccc
    25501 aaccaaaact cgattgccaa tggtgcagcc gtggagcatc accttgtgac caacagtgac
    25561 atcttcgcca atggttaatg ggttgccatc tgggttgtac gaggatttat gagtgacatg
    25621 caacatactg ccatcctgga tattggtgcg tgctccgatc tgtacataat gtacatctcc
    25681 acgaatcaca acgagcggcc agatccccac atcatcagcc agacgaacgt caccaatcac
    25741 gacactgcta tcgtcgatca ttacgcgctg accgatttgt ggaaaaagat cgcggtatgg
    25801 gcgtaaaaca tcagacatac ttacctcagc aataaatgat ttactaatga ctttgggggc
    25861 attattggcc ttgtgcaagt cttttagtat gcaaaaaagc accgttttgt gtgcgaatgc
    25921 agcaaaaagg gtgaaaaaac aacaaacaga aaaaaagatc aaaaaagtac ttgtgcaaaa
    25981 aattgggatc cctataatgc gcctccgttg agacgacaac gtgaaacact tcacaagatg
    26041 gtcgtaacaa cgaagagaaa aaatcctg
//
LOCUS       DAZFAB010000061        25820 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZFAB010000061 DAZFAB010000000
VERSION     DAZFAB010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25820
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.061"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(68..148)
                     /gene="shoB"
                     /locus_tag="ACHJWA_002871"
     CDS             complement(68..148)
                     /gene="shoB"
                     /locus_tag="ACHJWA_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFO4309431.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(570..950)
                     /gene="acpS"
                     /locus_tag="ACHJWA_002872"
     CDS             complement(570..950)
                     /gene="acpS"
                     /locus_tag="ACHJWA_002872"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFO4309432.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWEIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVANMHVTLADERHYACATVIIES"
     gene            complement(950..1681)
                     /gene="pdxJ"
                     /locus_tag="ACHJWA_002873"
     CDS             complement(950..1681)
                     /gene="pdxJ"
                     /locus_tag="ACHJWA_002873"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFO4309433.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(1693..2421)
                     /gene="recO"
                     /locus_tag="ACHJWA_002874"
     CDS             complement(1693..2421)
                     /gene="recO"
                     /locus_tag="ACHJWA_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFO4309434.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGVTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(2433..3338)
                     /gene="era"
                     /locus_tag="ACHJWA_002875"
     CDS             complement(2433..3338)
                     /gene="era"
                     /locus_tag="ACHJWA_002875"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFO4309435.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVATIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(3335..4015)
                     /gene="rnc"
                     /locus_tag="ACHJWA_002876"
     CDS             complement(3335..4015)
                     /gene="rnc"
                     /locus_tag="ACHJWA_002876"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFO4309436.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(4288..5262)
                     /gene="lepB"
                     /locus_tag="ACHJWA_002877"
     CDS             complement(4288..5262)
                     /gene="lepB"
                     /locus_tag="ACHJWA_002877"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFO4309437.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(5278..7077)
                     /gene="lepA"
                     /locus_tag="ACHJWA_002878"
     CDS             complement(5278..7077)
                     /gene="lepA"
                     /locus_tag="ACHJWA_002878"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFO4309438.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(7275..7754)
                     /gene="rseC"
                     /locus_tag="ACHJWA_002879"
     CDS             complement(7275..7754)
                     /gene="rseC"
                     /locus_tag="ACHJWA_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFO4309439.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(7751..8707)
                     /gene="rseB"
                     /locus_tag="ACHJWA_002880"
     CDS             complement(7751..8707)
                     /gene="rseB"
                     /locus_tag="ACHJWA_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFO4309440.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(8707..9345)
                     /gene="rseA"
                     /locus_tag="ACHJWA_002881"
     CDS             complement(8707..9345)
                     /gene="rseA"
                     /locus_tag="ACHJWA_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFO4309441.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHEWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(9378..9953)
                     /gene="rpoE"
                     /locus_tag="ACHJWA_002882"
     CDS             complement(9378..9953)
                     /gene="rpoE"
                     /locus_tag="ACHJWA_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFO4309442.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(9950..10105)
                     /gene="rseD"
                     /locus_tag="ACHJWA_002883"
     CDS             complement(9950..10105)
                     /gene="rseD"
                     /locus_tag="ACHJWA_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFO4309443.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            10361..11983
                     /gene="nadB"
                     /locus_tag="ACHJWA_002884"
     CDS             10361..11983
                     /gene="nadB"
                     /locus_tag="ACHJWA_002884"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFO4309444.1"
                     /translation="MNTLLEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHIQPNGEESYHLTREGGHSHRRILHAADATGREVETTLVSKALNHPNIRVLERS
                     NAVDLIISDKIGLPGTRRVVGAWVWNRNKEKVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDVRGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPYAHDISTLPPWDESRVENPDERVIIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(11968..12705)
                     /gene="trmN"
                     /locus_tag="ACHJWA_002885"
     CDS             complement(11968..12705)
                     /gene="trmN"
                     /locus_tag="ACHJWA_002885"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFO4309445.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAATQAQENVAQSPWLERINVHT
                     ADIQQWVTQQTARFDLIISNPPYYEQGVECATPQREQARYTTSLDHQTLLTCAAECIT
                     EEGFFCVVLPEQIGNSFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            12837..14171
                     /gene="srmB"
                     /locus_tag="ACHJWA_002886"
     CDS             12837..14171
                     /gene="srmB"
                     /locus_tag="ACHJWA_002886"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFO4309446.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(14204..15085)
                     /gene="yfiE"
                     /locus_tag="ACHJWA_002887"
     CDS             complement(14204..15085)
                     /gene="yfiE"
                     /locus_tag="ACHJWA_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4309447.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEHSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEDCFLPG"
     gene            15188..15775
                     /gene="eamB"
                     /locus_tag="ACHJWA_002888"
     CDS             15188..15775
                     /gene="eamB"
                     /locus_tag="ACHJWA_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFO4309448.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATTHGFHQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQT
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWIVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLIYCAVRIFY"
     gene            complement(15831..16214)
                     /gene="grcA"
                     /locus_tag="ACHJWA_002889"
     CDS             complement(15831..16214)
                     /gene="grcA"
                     /locus_tag="ACHJWA_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFO4309449.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            16519..17208
                     /gene="ung"
                     /locus_tag="ACHJWA_002890"
     CDS             16519..17208
                     /gene="ung"
                     /locus_tag="ACHJWA_002890"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFO4309450.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIATPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQHGETPI
                     DWMPVLPAESE"
     gene            complement(17256..18293)
                     /gene="yfiF"
                     /locus_tag="ACHJWA_002891"
     CDS             complement(17256..18293)
                     /gene="yfiF"
                     /locus_tag="ACHJWA_002891"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO4309451.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            18500..18919
                     /gene="trxC"
                     /locus_tag="ACHJWA_002892"
     CDS             18500..18919
                     /gene="trxC"
                     /locus_tag="ACHJWA_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFO4309452.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            18988..19686
                     /gene="tapT"
                     /locus_tag="ACHJWA_002893"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             18988..19686
                     /gene="tapT"
                     /locus_tag="ACHJWA_002893"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFO4309453.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            19718..22378
                     /gene="pat"
                     /locus_tag="ACHJWA_002894"
     CDS             19718..22378
                     /gene="pat"
                     /locus_tag="ACHJWA_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFO4309454.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATEIAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAKAHLLLQQAIAEGATSLDTHEVQPILQSYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMTWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDIAPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            22492..23847
                     /gene="pssA"
                     /locus_tag="ACHJWA_002895"
     CDS             22492..23847
                     /gene="pssA"
                     /locus_tag="ACHJWA_002895"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFO4309455.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            23893..24216
                     /gene="yfiM"
                     /locus_tag="ACHJWA_002896"
     CDS             23893..24216
                     /gene="yfiM"
                     /locus_tag="ACHJWA_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFO4309456.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKEFWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(24213..25511)
                     /gene="kgtP"
                     /locus_tag="ACHJWA_002897"
     CDS             complement(24213..25511)
                     /gene="kgtP"
                     /locus_tag="ACHJWA_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFO4309457.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDSALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYVVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(25628..>25820)
                     /locus_tag="ACHJWA_002898"
     CDS             complement(25628..>25820)
                     /locus_tag="ACHJWA_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4309458.1"
                     /translation="KAIRQDGLFALVKKMPDATLRVLYSHICQIQQRIRFPQLAYFHT
                     CPPYQKFILKLLNNHFPAN"
BASE COUNT         6238 a         6824 c         6455 g         6303 t
ORIGIN      
        1 ttatttatag ttattaaagg cgcgtccgat tggttcaccg gacgcacctt aagtacgttt
       61 ccttgtgtta taagaacaga aggatcagct gtaaaacagc aatgatgatt ttgatgaccc
      121 gtttaatcag gtatcggcaa tcagtcattc gtttttcctt aaacaaggaa tgcagccgtt
      181 cagattagcc cttacatctc cccaaaactg aacgtgcgag ttattggaat cgagtgctgc
      241 actccacacc agagctttga cgacaccact cgtttcaatg ggggaattca gtggcatggt
      301 gtaaagcaca gcaaaattgc caataacgaa accgatttta atgtacttat caaaaagcgg
      361 tcacgttaat cttttcatca atgtcgattt cttcgtaaag gctcgcgttt tttatgtcag
      421 agattgcaag ttgcttgtac aggtcaacga aattgatcca taatcatttt cgttggaatc
      481 gtgtgctgca caaaattaaa gttaaaaagt aaaacccccg ttccttacca gttcgggggt
      541 tttactttat aaagagaacg gtattatttt taactttcaa taattaccgt ggcacaagca
      601 tagtggcgct catcggccag cgtcacatgc atatttgcaa cgcccagctt ttccgccagt
      661 tttaatgcct cgccccacag tcgcagccgt ggcttaccaa gctcatcgtt aaatacttca
      721 aactgattaa acgccagacc attgcggatc ccggtgccaa acgcttttgc tgcggcttct
      781 tttacggcaa agcgtttcgc cagaaaacgc accggctggt ggtgcgtttt ccaaatttcc
      841 cattcgttat cgcttaatac gcggcgtgcc aggcgctcac cggatcgggc gatcactgct
      901 tctatgcgag cgatctctac gatatccgtg cctaaaccta atattgccat tagccacgcg
      961 cttccagcat caggcgcttc atttctgcca ccgcatcttt cagtccggtc atcactgcac
     1021 gaccaataat ggcatgaccg atattcagtt cgtgcatctc agggatggcg gcaatggctt
     1081 ttacgttgtg ataggtcaga ccgtgtccgg cgttaacttt caaaccgaga ctagcggcaa
     1141 aggtcgcggc tttggcgata cgcgccagct cttgcgcctg ttcggcgtca gttttggcat
     1201 cagcatagca accggtgtgg atctcgataa acggtgcgcc aacctctgcc gcagctttga
     1261 tctgctcttc atcggcgtcg ataaacagag aaacctgaat cccggcatcc gccagacgtt
     1321 tgcaggcatc gcgcattttg tcacgctgcc ctgcaacgtc caggccgcct tcggttgtta
     1381 cttcctgacg cttttctggt accaggcagc aaaaatgcgg cttcgtctca accgcgatcg
     1441 ccagcatctc ttcggtcacc gccatctcca gattcatgcg ggtatccagc gtctgacgca
     1501 ggatgcgcac atcgcggtca gtaatgtggc ggcgatcttc acgtaaatgc acggtaatgc
     1561 cgtccgcccc cgcctgctcg gcaataaacg cggcctgcac cggatccggg taagcggtac
     1621 cgcgcgcgtt gcgcagcgta gcgatatggt caatgttgac gcctaacagt aattcagcca
     1681 tgacaatcct catcattcat aatgtgtttt caccgttcgc ttaggcataa attgccggaa
     1741 cagttccctg ctctttaaag gtttaccgcc aagatacggc ttaagcgcca tgcgggtaaa
     1801 gcgtttcgcg gcgcgcagtg tgtctgcgtc aggaaattcc cgtgcgttta acgcttttaa
     1861 ctgccttccg gtgaacgttt tattgtcgat aacgacgctt gcgataaacc ctttttcttc
     1921 gcgataacga tacgtcatgg tgtcatctac cggctcgccg ctacccgcac aatgggtaaa
     1981 attgacgcca taacccagat gcccgagcag tgccagttca aagcggcgca gcgcgggttc
     2041 tggcgtacca gtgacccctg caagagactg aatgcagtgc aagtaatcga aaaagagttc
     2101 agagaagcgc gtctcgtatt ccagtacgcg ggagagaagt tcgttgatgt acagaccgct
     2161 gtaaagcgtg ataccgctta atggcagcgc cagcgagaca gcttcagcac tgcgcagcgt
     2221 tttgacttcg ccacgcccgc caaaacgtag caagagaggg gtgaaaggct gtaatgcacc
     2281 tttcagggta gagcgtttag agcgtgcgcc tttggcaacc agacgcacgc gacccgattc
     2341 ctccgtgaag acgtccagca tcaggctggt ttcgctccac gggcgactat gcaggacaaa
     2401 tgcgcgctgc cagccttcca tcggagttac tcttaaagat cgtcaacgta accgagactg
     2461 cgcagtgcgc gttcgtcgtc ggcccaaccg gatttcactt ttacccacag ctcaaggtga
     2521 acaggcgctt caaacatttc ctgcatgtct ttacgcgctt caatcccgat ggttttgatc
     2581 ttggcccctt tgttgccaat gaccatcttc ttctgccctt cacgctcaac gagaatcaaa
     2641 ccgttgatgt cataaccacc gcgttcgtta gagacgaaac gttcgatctc cacggtcacg
     2701 gagtacggca gttcagcgcc gaggaaacgc atcagttttt cgcggatgat ttcagacgcc
     2761 ataaaacgct gtgagcgatc ggtgatgtaa tcttccggga agtgatgagt cgcttcaggt
     2821 agatgcttac gcacgattgc cgcaatagtg gcaacattca gcccggtttc ggcagagatt
     2881 ggcacgatat cgaggaagtt catctggctt gccaggaact gcaggtgcgg cagcagatcg
     2941 gctttctcct gcacgttgtc cactttgttc accgcgagga ttaccggcgc tttgccttcg
     3001 cgcagtttgt tgagcaccat ttcgtcgtcc ggcgtccagc gggtgccttc aacgacaaaa
     3061 atcaccagct caacatcgcc aatagagctg ctcgccgctt tgttcatcag gcggttaata
     3121 gcgcgttttt cttccatatg caggcccggt gtatcgacgt agatcgcctg atacgcgcct
     3181 tcagtatgga tccccacaat gcggtgacga gttgtctgcg ccttgcggga ggtgatggag
     3241 attttctgcc ccagcagttt gttcaacaat gtggatttgc caacattcgg acgtccgacg
     3301 atggcaataa atccgcagta acttttatcg atgctcattc cagctccagt tttttcaacg
     3361 cctgttcggc ggcagcctgc tcagccttac gacggcttga acctgtgcca accaccggtt
     3421 cactcaggcc gctgacctgg cagtggattg taaattcctg atcgtgcgct tcgccacgga
     3481 cctgaactac cagataagtc ggcagcggca gatgacgacc ctgcaaatat tcttgcaagc
     3541 gcgttttcgg atctttttgt ttatcgcctg ggctaatttc gtccagacga gtttgatacc
     3601 agttgaggat taatttctcg acggtttgaa tatcactgtc gaggaatacg ccaccaatta
     3661 atgcttcgac ggtgtcggcg agaattgact cacgacgaaa tccaccgctt ttaagttcac
     3721 ctggtcctaa acgtaagcac tcgcctaact caaattcgcg cgccagttcc gccagcgtat
     3781 tgccacggac cagcgtggcg cgcatccggc tcatatcgcc ttcatccaca cgagggaaac
     3841 ggtgataaag cgcattggcg ataacgtagc tcagaataga gtcgcctaaa aattctaaac
     3901 gctcgttatg tttgctgctg gcactacgat gagttaatgc ctgctgcaac agttcctgat
     3961 gattaaaagt gtagcccagc ttccgttgaa gccgattaat tacgatgggg ttcatgcgat
     4021 accaataaat aaatgcgtca acaattcagc acacgaaaca gacctgatat acatggctct
     4081 gctaactgct tcgctgcagt ttcagtttag cggtatatgg ggaccaacgc tgtttcgtgt
     4141 gccgtggcaa cctggaggtg ccaaccttaa acttcggggg aatattctat acacaacgac
     4201 gggggatgtc gttagccacg ggagatttat ctcataaata attcacgttg tcgccataac
     4261 ggcgacaacg tgaacgaaga tgagctatta atggatgccg ccaatgcgac ttaagcgcac
     4321 acctgtcggc cattcgcctt cttgcttatc gaagctcatc cagatagccg ttgcccgacc
     4381 gaccagattc gcttcaggca caaagcccca gtaacggctg tccgcgctgt tgtcgcggtt
     4441 gtcgcccatc atgaagtatt gtcccggagg aacaatccag gttgccagtt gttgccctgg
     4501 ctgctggtaa tacatcccca cctgatcctg cgcaatcggc actgtcagaa tgcggtgcgt
     4561 cacatcaccc agtgtctctt tacgctcgga aagacgaatt ccattttctt tggtttcgtt
     4621 tttcggcact tcaaagaatc cgctggtcgc ttccccacca ttacggcgtg agaaggtctg
     4681 aacgaaatcg ctcggttcca cgtttgagta ggtgaccggc agcgcgtttt cacacgcctg
     4741 gccggaactg catcccggtt gaatcgtcag ctcttttgag accggatcgt aagtgacttt
     4801 atcgcccggt aaacccaccg cgcgcttgat ataatcaagc tttggatctt ccggatattt
     4861 aaagaccacg atatcgccgc gtttcggatg accggtttcg atcagcgttt tctggtagat
     4921 aggatcttta atgccataag caaacttctc taccagaata aaatcaccga ttaacagagt
     4981 cggcatcatc gaacctgacg gaatctggaa cggttcataa ataaacgaac gcacaatcaa
     5041 tacgatagcc agtaccggaa aaacagaagc accggtttcc agccagccag gcttcggcgc
     5101 aacctttttc aacgttgctt tatccagtga gtcaccggca gccgcctgcg ccgctgcctg
     5161 acgttcccgc cgtttaggtg cgaaaaagaa tttatccacg caccataaaa tgcccgtcac
     5221 cagtgtggca atcaccagaa tcagggcaaa catattcgcc atgccaactc ctaagggtta
     5281 tttgttgtct ttgccgacgt gcagaatggc gaggaacgct tcctgcggca gctcgacgtt
     5341 accgatctgc ttcatgcgtt tcttaccttc tttctgcttc tgcagcagct ttttcttacg
     5401 gctgatatcg ccgccataac atttagccag tacgttttta cgcagctgtt tcacggtgga
     5461 tcgcgcaata atgtgcgtac caatcgctgc ctgaatggcg atatcaaact gctggcgtgg
     5521 gatcagatct ttcatcttct ccaccaactc gcgaccgcgg ttttgcgaat tatcacggtg
     5581 ggtgatcaac gccagcgcat caacacgttc accgttgatt aatacgtcta cacgtaccat
     5641 gtcggacgcc tggaagcgtt tgaagttgta atccagagac gcataaccac gcgaggtaga
     5701 tttcaggcga tcgaagaagt cgagcaccac ttccgccatc gggatctcgt acgtcagcgc
     5761 cacctgatta ccgtggtaaa ccatattggt ctgcacgccg cgtttttcta cgcacagcgt
     5821 aataacgttg ccgagatatg cctgcggcag cagcatgtga cactctgcaa tcggctcgcg
     5881 cagttcgtaa atgttattta ccgcaggcag cttggatggg ctgtcgacgt agataacttc
     5941 ccttgacgtg gtttcaactt catacactac ggtcggcgca gtggtgatca gatccagatc
     6001 gtattcacgt tccagacgct cctggatgat ctccatgtgc agcaggccaa ggaagccgca
     6061 gcggaaacca aagcccagcg cgctggagct ttccggctca tagaacagtg aggcatcgtt
     6121 caggctgagt ttacccagcg cgtcacggaa ggcttcatag tcgtcggaac ttaccgggaa
     6181 cagaccggcg tatacctgcg gtttgacttt cttaaagcca ggcagcgcct tttctgccgg
     6241 attacgcgcc agcgttaagg tatcgccgac tggagcgccg tggatatctt taatcgcaca
     6301 tacgagccag cctacttcgc cacatttcag ttcagtgcgg tcaacctgtt tcggcgtgaa
     6361 gatgcccaga cggtcggcgt tataggtctg cccggtactc atgactttca ctttgtcgcc
     6421 cttacgcagg gtgccgtttt taatacggat aagtgaaaca acgcccaggt agttgtcgaa
     6481 ccatgagtcg ataattagtg cctgcaacgg gccttccgga tcgccttccg gcggcggaat
     6541 gtcgcgcacc agacgttcga gaacgtcctg cacgccaacg ccggttttcg ctgaacagcg
     6601 caccgcgtca gtggcgtcga tgccgacgat atcttcaatt tcttccgcca cgcgttcagg
     6661 atcggctgcc ggcaggtcaa tcttgttcag taccggcacc acttcgagat ccatttccat
     6721 ggcggtgtag cagtttgcca gggtttgcgc ttccacgccc tgcccggcgt cgaccaccag
     6781 caatgcacct tcacaggcag ccagcgaacg ggaaacttca taggagaagt ctacgtggcc
     6841 cggggtgtcg ataaagttaa gctgataggt ttcgccgtca gacgctttgt agtccagcgt
     6901 cacgctttgc gctttgatgg taatgccacg ctcacgctca agatccatgg aatcgagaac
     6961 ctgcgcctcc atttcacggt cagacaggcc accgcagatc tggataatac ggtcagacag
     7021 cgtcgattta ccgtggtcaa tgtgagctat gatcgaaaag ttacgtatat tcttcataaa
     7081 gtatgattat tgtgccttaa tgcccggtta accaggcttt tagaagtcgc tgttctgagc
     7141 ttaacgtctg tattaataga aacgccgcat tctacactac aacattgagg cgaggaaatg
     7201 ttcataccgt atggattgtg gtatctggaa acgtcctcgc atttgttatg caaaatgcaa
     7261 caaagccagt gaaatcactg gctcgcgtct tccgaagatg tttcaaatcg cacaaggcca
     7321 ggcggcaatg ccacgcttaa gatgatcggt tgccattctg cccgggcagc aaacttacgc
     7381 gagtagccac gcgcaatcag gaacccgcca ataccgccga ggaccgcacc acataatgct
     7441 gcgacgtcag aagcaaagag tagctgaaat agcgaagcga tgaggaataa tcccaccagc
     7501 ggcgacatat aaaccagtaa tgcggagcta agcaggctgc cttcggcgat ccccaattcc
     7561 actttttgcc ccggcactaa cggctcatca cagggtacga caatggtatg cgtggtttgc
     7621 gggccaagtt tattcaacac gcggctaccg caaccggcgc gtgaagcgca gctgctgcat
     7681 gaggctttaa catcacaact gaccagcgcc tgcccgtttt gccaggagac gacggtagcc
     7741 cactctttga tcattgcgct gccccgaact taatattctc ggcaatgcgt ttcgccgttt
     7801 gcggcggcag ttcaccgaca atggtgattt cggcgttatc acgtacgctt gtactgacgg
     7861 ttctgcgtcc ggtgcgcaac atctgatcgg tgctcgatgg cgtagcgcgg ttaacgttta
     7921 ccgagaagct gaataatccg tcggaataga gacgtgattc gataggcatg ttgtccatcg
     7981 tcggtagcgg acgtcgacta ctggaaactt cgctaaaacc ctgtggcaac caggttggcg
     8041 tccagctgaa tttagctttt tcacctacag gaacagaaag caacggcggc aaatttgcct
     8101 tcgccagcgt ctgcatactg ctgctgatat cctgattgac gttaaaagca atcacgcgaa
     8161 attgttccag cgtttcacca tcgcgatcaa gaagatcaac ccgcatcggt aatttcgatt
     8221 cggtgtccat ccacacgatg tagctgtagc gtgtaccatc tcgggcaacc acgcgaatga
     8281 cttcgcaaag acgatcagca atacgcgtgc gcccgacgga gataaagtcg tagtaaggag
     8341 aaaggcgttt gaaatcggta tagatgagcg atggcagaga atcaacaatg taatcgccat
     8401 taagcgtgaa cggttcaagt cccggttcaa aatagctgat ttcattgccg cgctgtacca
     8461 cttcccggcg cgggccatcc atttgcaaca attgtgcaag aggacggtta tcgaggcgtg
     8521 catgtcgata acgcagagac tcaacaccct gtttattgat gctgatgaat gacagctcgt
     8581 aattcagtga ctgactggcc aggttcatct gctgtaataa cgccccggac gcgggagtgg
     8641 ccgaggcgtt agcagagaat aacaggctac ctgtcactaa tgacatggca aaccaaagtt
     8701 gcttcattac tgcgattgcg ttcctaaagt ttgaattcct ggcacctgta cagcggcttg
     8761 ctgggtttgc gcctgctcaa actgaagctg ttcagagtgg agtcggcgtt gcagttcgta
     8821 atcctgcaac attgcattaa tgcgacgacg ctgctgctgt tgaccattat ttgcggtcgc
     8881 ttcagaaggt actcccaggc taaccgggct ggctttaccc atcatcggca gtgtattaaa
     8941 taccggcgtt tcgggctgct gggacgtttc agattgtcca ttatagtgct ggacgccaac
     9001 gataactgca agcgatacgc atgcggctac gcccatttgg gtaagctgtg ccgcccacgg
     9061 acgtactttc tgccagaatg gcattttctg ccattcatgc ggcgcaggct gggcttccgg
     9121 gatcaatgtc gccggttgac gtactggctc ttcttcaatg gcggccatca cgcgtgaaga
     9181 gatatcgaaa tggagcacct cgggagtatc accccgcatt gagtcacgga ttaagtgata
     9241 gctttcccag gttttctgca tttctgggtt atgagccagt tcgttaagca gctcactatc
     9301 cagcgtttcg ccatccatta aagcggaaag ttgttctttc tgcatgccta atacccttat
     9361 ccagtatccc gctatcgtca acgcctgata agcggttgaa ctttgttatc aatagcttcc
     9421 ctcgctcgga agatacgtga acgcaccgta cctaccggac aatccatgat agcggctatc
     9481 tcttcatagc tcaggccatc cagctcccgc aaggttattg ccatgcgtaa atcttccggg
     9541 agggactcaa tagttcggaa aactatctgt ctcagttctt ctgacaacat taagttctca
     9601 gggttcgaaa tttctttcaa cgcgccgcca ctttcgaagt tttcagcttc aatggcatcc
     9661 acatcactgg aaggtggacg acgcccctga gcaaccaggt aatttttcgc tgtatttaca
     9721 gcaatccgat acagccatgt ataaaaagcg ctatctcccc ggaacgaatc cagcgcacga
     9781 taggctttaa taaaagcttc ttgtaccaca tcgggaacat cacccgacgg cacatagcgg
     9841 gaaaccagac tcgccacttt atgctgatag cgcactacca gtaagttaaa ggctttctga
     9901 tctcccttct ggacccgttc aaccaggacc tggtccgtta actgctcgct catccgaggt
     9961 aaagtctccc caaaccaaat ttccacgcgc tatcgaaacg ccactccatt agctgcaatt
    10021 tgagcaagca aagggttaga gtgtctcgtt tttgtaaagt tccgtaacgc atctgttttt
    10081 gtttgtcatg ctgtagacgg atcattatct atcattataa gtctacagaa tctgaacatc
    10141 gcattatctg tgtagaaatg cccatttaac tgcctgaaga gtaacccaac ggccttttta
    10201 tttcaccacc taatcctcca ccagccagta acttctcttt ttctcgccgc cctgcgtcag
    10261 cgtgtttagt cactgtaaca aatattaaaa tagcaggtgt ttattcgcac aacatgatgc
    10321 tatgctgacc aaaccgtgtt tagtaaatta aacaaagaaa atgaatactc tccttgaaca
    10381 ttcatgtgac gtgttgatta tcggtagcgg cgcagccggg ctttcactgg cgctacgcct
    10441 ggctgaccag catcaggtca tcgttctaag taaaggcccg gtaacggaag gttcaacatt
    10501 ttatgcccag ggcggtattg ccgccgtgtt tgatgaaact gacagcattg actcgcatgt
    10561 ggaagacaca ttgattgccg gggctggtat ttgcgatcgc catgcagttg aatttgtcgc
    10621 cagcaatgca cgatcctgtg tgcaatggct aatcgaccag ggggtgttat ttgataccca
    10681 cattcagccg aatggcgaag aaagctacca tctgacccgt gaaggtggac atagtcaccg
    10741 tcgtattctt catgccgccg acgcaactgg tagagaagta gaaaccacgc tggtgagcaa
    10801 ggcgctgaac catccgaata ttcgcgtgct ggagcgcagc aacgcggttg atctgattat
    10861 ttctgacaaa attggcctgc cgggcacgcg acgggttgtt ggcgcgtggg tatggaaccg
    10921 aaataaagaa aaggtggaaa cctgccacgc aaaagcggtg gtgctggcaa ccggcggtgc
    10981 gtcgaaggtt tatcagtaca ccaccaatcc ggatatttct tctggcgatg gcattgctat
    11041 ggcgtggcgc gcaggctgcc gggttgccaa tctcgaattt aatcagttcc accctaccgc
    11101 gctgtatcac ccacaggcac gcaatttcct gttaacggaa gcactgcgcg gcgaaggcgc
    11161 ttatctcaag cgcccggatg gcacgcgttt tatgcccgat tttgatgtgc gcggcgaact
    11221 ggccccgcgc gatattgtcg cccgcgccat tgaccatgaa atgaaacgcc tcggcgcaga
    11281 ttgtatgttc cttgatatca gccataagcc cgccgatttt attcgccagc attttccgat
    11341 gatttatgaa aagctgctcg ggctggggat tgatctcaca caagaaccgg taccgattgt
    11401 gcctgctgca cattatacct gcggtggtgt aatggttgat gatcatgggc gtacggacgt
    11461 cgagggcttg tatgccattg gcgaggtgag ttataccggc ttacacggcg ctaaccgcat
    11521 ggcctcgaat tcattgctgg agtgtctggt ctatggctgg tcggcggcgg aagatatcac
    11581 cagacgtatg ccttatgccc acgacatcag tacgttaccg ccgtgggatg aaagccgcgt
    11641 tgagaaccct gacgaacggg taataattca gcataactgg cacgagctac gtctgtttat
    11701 gtgggattac gttggcattg tgcgcacaac gaagcgcctg gaacgcgccc tgcggcggat
    11761 aaccatgctc caacaagaaa tagacgaata ttacgcccat ttccgcgtct caaataattt
    11821 gctggagctg cgtaatctgg tacaggttgc cgagttgatt gttcgctgtg caatgatgcg
    11881 taaagagagt cgggggttgc atttcacgct ggattatccg gaactgctca cccattccgg
    11941 tccgtcgatc ctttctcccg gcaatcatta cataaacaga taaaaagcct gggttagcgc
    12001 cgtatacgct tcggaatagt tctggtctgg cccacgaatg actaagcgat cgctaaagca
    12061 ttctcccgcc tgcggggaga atgccagcag cacccgatgc ggcagtcgcg cttcgttttc
    12121 cgccacatcc gtccgcaaac gtaaatgcca gcccatactt aatgccagct ccgtaaaact
    12181 attaccaatc tgctctggca gcaccacgca gaaaaatccc tcttcggtaa tgcactccgc
    12241 cgcgcaagta agtaaagtct ggtgatcaag tgaggtggtg tagcgcgcct gttcccgctg
    12301 cggcgtcgcg cactccactc cttgctcata atacggcggg ttgctgatga ttaaatcaaa
    12361 gcgcgcggtc tgctgtgtta cccactgctg aatatccgcc gtatggacgt taattctctc
    12421 taaccacggg gactgggcga cattttcctg cgcctgagtg gcagcttcac tttccagctc
    12481 aacggcatca attatcacgc tgtcactggt tcgctgcgcc agcatcagtg ccagcaagcc
    12541 actacccgca ccgatatcaa ggcaacgttt taccccagcc accggtgccc atgcgcccag
    12601 caatatgcca tccgttccca ctttcatcgc acagcgatcg tgagcaacaa aaaactgttt
    12661 aaaagtaaat ccattacgac gaagcacgga tgtagactgt gacatgaaaa taaaaccttg
    12721 caggaaaaac ggcgatagca ccgggtgaga acaatacccg agaagcgata tccatacaaa
    12781 cagatgaaga ttgcagccgt aacgtctata atcagcgccc cacacagagg tagaacatga
    12841 ctgtaacgac tttttccgaa cttgaactcg acgaaagcct gctggaagcc ctccaggata
    12901 aaggtttcac tcgcccgacc gccattcagg ctgccgccat tccgcctgcg ctcgatggcc
    12961 gtgatgtact cggttctgcg ccgacaggca ccggtaaaac ggcggcgtat ctgctgccag
    13021 cgttgcagca cctgctcgat ttcccgcgta agaaatccgg tccgccgcgt attttgatcc
    13081 tcaccccaac tcgcgagctg gcgatgcagg tggccgatca tgcccgcgaa ctggcgaaac
    13141 atacgcatct ggatatcgcc accatcaccg gcggcgtagc ctatatgaac cacgcggaag
    13201 tgttcagcga aaatcaggac atcgtggtcg ccacgaccgg acgtctgctg caatacataa
    13261 aagaagagaa cttcgattgc cgcgcggttg aaacgctgat cctcgacgaa gcagaccgta
    13321 tgctggatat gggcttcgct caggatatcg aacatattgc tggcgaaacg cgctggcgta
    13381 aacagaccct gctcttttcg gcaacgctgg aaggcgatgc gattcaggac tttgccgagc
    13441 gtctgctgga agatccggtg gaggtttctg ccaatccgtc cacccgtgag cgcaaaaaaa
    13501 ttcatcagtg gtattaccgc gccgatgacc tggaacataa aaccgcattg ctggtgcatc
    13561 tgttaaaaca gccggaagcg acccgctcaa ttgtgtttgt gcgtaagcgt gagcgtgtgc
    13621 atgagctggc aaactggctg cgcgaagcgg gcatcaacaa ctgctatctc gaaggtgaga
    13681 tggtacaggg caagcgtaac gaagcgatca aacgtttgac cgaaggtcgc gtaaacgtac
    13741 tggttgcaac cgatgttgcc gcgcgcggta tcgacattcc tgacgtcagc cacgtcttta
    13801 acttcgatat gccgcgcagt ggcgatactt atttgcaccg tatcggacgt accgcgcgcg
    13861 ccggtcgtaa aggcaccgca atttcgctgg tagaagccca tgaccatctg ctgctgggca
    13921 aagttggtcg ctatattgaa gagccaatta aagctcgcgt aattgatgag ttacgcccga
    13981 aaacgcgtgc accaagcgag aagcagaccg gcaagccatc gaagaaagta ctggcaaaac
    14041 gcgccgagaa gaaaaaagct aaagagaaag agaagccgcg ggtgaaaaaa cgccatcgcg
    14101 ataccaaaaa tattggtaag cgccgtaaac caagcggaac gggcgtgcca ccgcaaacga
    14161 cagaagagta atctcaatgc cgggtttaag cccggcatta tttttatccc ggcaaaaaac
    14221 aatcttcaac acactgaata aaagtgtgca tcgccgggct aaccgctttt ccggcatggt
    14281 gagcgcacat tgccgtaatg gtctgcgagt gttcgccaaa aggcaattca attaattctc
    14341 cgctctcaag ctctttttcc accgcaaagc ggggcaagta actgacaccg atattcgccg
    14401 cgacacaacg tttgatactt tcaatgctta acagctcaat ggtgttttcc accgtgatcc
    14461 gccgctggcg cagcgtgctc tcaaatatct gccggaatac acattgcggt tcgttgataa
    14521 taaagctaca ggcgttatgt cttcccggct cagtaaaatc gacatctgca atttgcggtg
    14581 aagctaccag caccagcgat tgctcaccca attctcgtcg gttcagggca tcatcattcc
    14641 ctacgcgata aaaaaccccg acatcggcct catcattcaa tagcgcatca cggatcacgt
    14701 aacagttcag agactgcaac gataaacgca cttttggggc gcgttgccga aaacgctgca
    14761 acacctgcgg cattcggtaa gagagcaacg tttcgcccga aacaacgcgt agttccccgt
    14821 ccggatccga ctcctttttg gcagcctcac gaagggtatc catcactctg gtgagttcat
    14881 aaatgtgcgg cagcagcttt ttaccttcgc gagtgaggca catccgtcgg ccaattttct
    14941 caaataactg taccgagaac tcctgctcaa gctgctgaat atgaaaagtc accgtcgatt
    15001 gtgtacagca caatttttgc gaagctcgca aaaaggaacc ctcttccacc acggttttaa
    15061 gcgtaataaa acggcgcaga tccataaccc caaacctatc gaaaatatcg aatttagaat
    15121 gtaaaaacat tcattttttt aaatattctg tgtcgggtac tgtctaccaa aacagaggag
    15181 ataacaagtg acaccgaccc ttttaagtgc tttttggact tacaccctga ttaccgccat
    15241 gacgccagga ccgaacaata ttctcgccct tagctcagcc acaacgcacg gatttcatca
    15301 gagtacccgc gtgctggcag ggatgagtct gggattttta atcgtcatgt tgctgtgtgc
    15361 gggcatttcg ttttcactgg ctgttatcga cccggcagct gtgcatctgt tgagttgggc
    15421 gggggcggca tatattgtct ggctggcgtg gaaaatcgct accagtccaa caaaggaaga
    15481 cgggcttcag acaaaaccaa tcagtttttg ggccagcttt gctttgcagt ttgtgaacgt
    15541 caaaatcatt ttgtacggtg ttacggcact gtcgacattt gttctgccgc aaacacaggc
    15601 gttaagctgg atagttggcg tcagcgtttt gctggcaatg attgggacgt ttggtaatgt
    15661 gtgctgggcg ctggcggggc atctgtttca gcgattgttt cgccagtatg gtcgccagtt
    15721 aaatatcgtg ctggcgctgc tgctgatcta ttgcgcggta cgcattttct attaatgaaa
    15781 aaaagcggaa gaggtcgccc tcttccgctt agtaacatgc tacttaagcc ttacaggctt
    15841 tcagtaaagg tacgagcgat aacgtcgcgc tgctgttccg gagtcagaga gttaaagcga
    15901 actgcataac cggatacacg gatggtcagc tgcggatatt tttccggatg cttaactgca
    15961 tcttccagag tttcacgacg cagaacgtta acgttcaggt gctgaccacc ttcaacgcga
    16021 acttctggtt tcacttctac tggaacttca cggtactcaa tgtcacccag tttgcttact
    16081 gcaaccactt cgtcttctgc ataacctgct ttcgcaacga tgcaacgcgc ttcgcctttt
    16141 tcgctgtcca gcagccagaa agagttcagc agatcgtcgt tagcggcttt agtaatctgg
    16201 atacctgtaa tcatgtgatg cctccccggc aaaattattt gatttgttca gcctgtcgcg
    16261 gccaattggt aaaaccattg ttgcttgagt gtatatatac tcctcaaaca cccttgaatc
    16321 tttgatttaa atcaataaaa accacacatc aagtgtggtc gcaaatagat tttattgttt
    16381 tacatcaact tatgcgggtg tgaaatttta ccaatttaca tttttttgca ctcgtttaag
    16441 tctaaaaaat gagcatgatt ttgttctgta gaaagaagca gttaagctag gcggattgaa
    16501 gattcgcagg agagcgagat ggctaacgaa ttaacctggc atgacgtgct ggctgaagag
    16561 aagcagcaac cctattttct taataccctt cagaccgtcg ccagcgagcg gcagtccggc
    16621 gtcactatct atccaccaca aaaagatgtc tttaacgcgt tccgctttac cgagttgggt
    16681 gacgttaaag tggtgattct cggccaggat ccttatcacg gaccgggaca ggcgcatggg
    16741 ctggcatttt ccgttcgccc cggaatcgcc actcctccgt cactgctgaa tatgtataaa
    16801 gagctggaaa atactattcc gggcttcacc cgccctaatc atggttatct tgaaagctgg
    16861 gcgcgtcagg gcgttctgct actcaatact gtgttgacgg tacgcgcagg tcaggcgcat
    16921 tcccacgcca gcctcggctg ggaaaccttc accgataaag tgatcagcct gattaaccag
    16981 catcgcgaag gcgtggtgtt tttgttgtgg ggatcgcatg cgcaaaagaa aggggcgatt
    17041 atagataagc aacgccatca tgtactgaaa gcaccgcatc cgtcgccgct ttcggctcat
    17101 cgtggattct ttggctgcaa ccattttgtg ctggcaaatc agtggctgga acaacatggc
    17161 gagacgccga ttgactggat gccagtatta ccggcagaga gtgagtaaat ttgcgggaaa
    17221 atgccggatg gcagagttgc cacccggctg atttatcagg ctttattctg acgccaccat
    17281 tcgccaagaa gaacgccggt tgcgacagag atattcagcc cggcaacgtt gccagtacca
    17341 tcaatcttca cgcgcagatc gttcggatcg cgtgcggcat ccggtaaccc ttcatattcc
    17401 tgacccagca ccagtaccat tttcgctggc agactggttt tgaacagcgg tttaccctgc
    17461 tcgctggaag ttgtcactac ggtgtaaccc gcctgacgga aatcatccag cacgttaaca
    17521 atgttgtcgc cagtaatcgg ctgaacgtgc tctgcgccgc cttctgcggt acggatagcc
    17581 gcccccgact ccagcagtgc cgcatcctgc accacaacac ctttcacgcc aaagtgcgcg
    17641 cagctgcgca tcatgccacc caggttatgc gggttagatt cgttttccag tgccagaaca
    17701 caatcctgcg cgcctgcctg gcttacccac tgctgcacgg ttgtgccatt acgctttttg
    17761 atcaggaagc aaacgccacc gtgatgttcc gtgcctgacg cttttgtcaa ttccgcttca
    17821 tctaccacat ggtacgcttt gcggtttgct gccatccagc gcaaggcttc tttaaaacgc
    17881 ggcgttacac tctggataaa ccaggcgcga acaatcgctt ccggacggct ctggaacagt
    17941 gcctgacagg cgttttcgcc gtagacgcgg gtttcttccg cacgctgacg acgcaacact
    18001 tccggatcaa taaaactttt accactgatg ccaccgtgat cggccttttc cggcgtctca
    18061 tcacccggcg cgcgggaaac cgtgcgccac ggcgaatctt cccacttgcg gtcacggggc
    18121 tgactctgtt tgtcatcgcg ggcggggcga cggccaccgt cagcacgaga ttttcctgga
    18181 cgcccgcccc ctttcccggt acgcgggttg tgggtacgtt tatcagaatc atcatcactg
    18241 cggacataca tcactttgac cttgccgctt ttacctttca tttcatcgtt catgcttttc
    18301 tccaccagcg ctgcgcgaag cgcgcagatt acccgaagtc cgcgcggttc gccatgattt
    18361 cgtaccaaag cctgcgacta tcatacctat tgaataaaac agattgttgt ctggaacaat
    18421 gtccccgata atatgtaaca tattagaaac ataccggcgt tgttgccgat aagtctcctt
    18481 actcatcccg aggttagtta tgaataccgt ttgtacccat tgtcaggcca tcaatcgcat
    18541 tcccgacgat cggatcgaag atgcggcaaa atgcggacgc tgcggtcacg acttgtttga
    18601 cggagaggtg attaatgcga ccggtgaaac gctcgacaaa ttgctgaaag atgatctacc
    18661 agtggtgatc gacttctggg caccgtggtg cggcccctgc cgtaatttcg caccaatttt
    18721 tgaagatgtc gcgcaagagc gtagcggtaa agtgcgcttt gtgaaagtga ataccgaagc
    18781 tgaacgtgaa ttgagcagtc gctttggaat tcgtagtata ccgacgatca tgattttcaa
    18841 aaacggtcag gttgtcgaca tgcttaatgg cgcagtaccg aaagcgccgt tcgatagctg
    18901 gctgaacgaa tctctttaat cttaccgggg cgcatcttgt gccccgtttt ctcctctgcg
    18961 acaatggcgt tttttcgacg ctctcttatg accgaaaacg ctgttctcca gttacgcgcc
    19021 gagcgtattg cgcgcgcaac acgtcctttt cttgcccgcg gtaatcgcgt tcgtcgctgc
    19081 caacgctgtc ttttgccaga gaaattatgt ctctgttcga caattacccc agcacaagca
    19141 aaaagtcgct tctgtttgct gatgttcgac accgagccaa tgaagcccag taataccggg
    19201 cgtctgattg ctgatatttt gcctgatacc gttgcgtttc aatggtcgcg taccgaaccc
    19261 tcgcaggatt tgctggatct ggtacaaaac ccggattatc agccaatggt ggtctttccc
    19321 gcctcgtatg ctgatgagca acgggaagtg atcttcacac cacctgccgg taagccaccg
    19381 ctgtttatca tgctcgatgg tacctggccg gaagctcgca agatgtttcg taaaagtccg
    19441 tatctggata atcttcccgt catttccgtc gatctttccc ggctttctgc ctatcgcctg
    19501 cgtgaagccc aggctgaagg ccaatattgt actgccgagg tagccatcgc actgttagat
    19561 atggccggcg ataccggggc ggcagcaggg ttaggcgagc acttcacccg ctttaaaacg
    19621 cgctatctgg caggaaaaac gcaacatctg ggtagcatca cagcagaaca gttagaaagc
    19681 gtttaaaatc attcggtcac ttctgcggga gaccggtatg agtcagcgag gactggaagc
    19741 actactgcga ccaaaatcga tagcggtaat tggcgcgtcg atgaaaccca atcgcgcagg
    19801 ttacctgatg atgcgtaacc tgctggcggg aggctttaac ggaccggtac tcccggtgac
    19861 gccagcctgg aaagcggtgt tgggtgtgtt ggcctggccg gatattgcca gcttgccctt
    19921 tacacccgac cttgcggttt tatgtaccaa tgccagccgt aatcttgctc ttctggaaga
    19981 gctcggcgag aaaggctgta aaacctgcat tattctttcc gccccggcat cgcaacacga
    20041 agatctccgc gcctgcgccc tgcgccataa catgcgcctg cttggaccaa acagtctggg
    20101 tttactggct ccctggcaag gtctgaatgc cagcttttcg cctgtgccga ttaaacgcgg
    20161 caagctggcg tttatttcac agtcggctgc cgtctccaac accatcctcg actgggcgca
    20221 acagcgtgag atgggctttt cctactttat tgcgctcggc gacagcctag atatcgacgt
    20281 tgatgaattg cttgactatc tggcacgcga cagtaaaacc agcgccatcc tgctctatct
    20341 cgaacagtta agcgacgcgc gacgctttgt ttcggcggcc cgtagtgcct cgcgtaataa
    20401 accgattctg gtgattaaaa gcggacgtag cccggcagca cagcgactgc tcaacacgac
    20461 ggcaggaatg gatccggcgt gggatgcggc gattcagcgt gccggtttgt tgcgggtgca
    20521 ggatacccac gagctgtttt cggcggtgga aaccctcagc catatgcgcc cgctgcgtgg
    20581 cgaccggttg atgattatca gcaacggtgc tgcgcctgcc gcgctggcgc tggatgcctt
    20641 atggtcacgc aatggcaagc tggcaacgct aagcgaagaa acctgccaga aactgcgcga
    20701 tgcactgcca ggacatgtgg caatctctaa cccgctcgat ctgcgcgatg acgccagcag
    20761 cgagcattat gtcaaaacgc tggacattct gctccacagc caggattttg atgcgctgat
    20821 ggttattcat tcgcccagcg ccgctgctcc cgcaacagaa atcgcgcaag tattaattga
    20881 agcggtaaag catcatcccc gcagcaagta tgtttctctg ctgacgaact ggtgcggcga
    20941 gcactcctcg caagaggcac gacgtttatt cagcgaagcc gggctgccga cctaccgtac
    21001 cccggaagga accatcactg cttttatgca tatggtggag taccggcgta atcagaagca
    21061 actacgcgaa acgccggcgt tgcccagcaa tctgacttcc aataccgcaa aagcgcatct
    21121 tctgttgcaa caggcgattg ccgaaggggc tacgtcgctc gatacccatg aagttcagcc
    21181 catcctgcaa tcgtatggga tgaacacgct ccctacctgg attgccagcg atagcaccga
    21241 agcggtgcat attgccgaac agattggtta tccggtggcg ctgaaattgc gttcgccgga
    21301 tattccacat aaatcggaag ttcagggcgt catgctgtac ctgcgtacag ccaatgaagt
    21361 ccagcaagcg gcaaacgcta ttttcgatcg cgtaaaaatg acctggccgc aggcgcgggt
    21421 ccacggcctg ttggtgcaaa gtatggctaa ccgtgctggc gctcaggagt tgcgggttgt
    21481 ggttgagcac gatccggttt ttgggccgtt gatcatgctg ggtgaaggcg gtgtggagtg
    21541 gcgtcctgaa gatcaagccg tcgtcgcact gccgccgctg aatatgaacc tggcccgcta
    21601 tctggttatt caggggatca aaagtaaaaa gattcgtgcg cgcagtgcgc tacgcccatt
    21661 ggatgttgca ggcttgagcc agcttctggt gcaggtttcc aacttgattg tcgattgccc
    21721 ggaaattcag cgtctggata ttcatccttt gctggcttct ggcagtgaat ttaccgcgct
    21781 ggatgtcact ctggatatcg cgccgtttga aggcgataac gagagtcggc tggcagtgcg
    21841 cccttatccg catcagctgg aagaatgggt agaattgaaa aacggtgaac gctgcttgtt
    21901 ccgcccgatt ttgccagaag atgagccaca acttcagcaa ttcatttcgc gggtcaccaa
    21961 agaagatctt tattaccgct actttagcga gatcaacgaa tttacccatg aagatttagc
    22021 caacatgacg cagatcgact acgatcggga aatggcgttt gtagcggtac gacgaattga
    22081 tcaaacggaa gagatcctcg gcgtcacgcg tgcgatctcc gaccctgata acatcgatgc
    22141 cgaatttgcc gtgctggttc gctcggatct caaagggtta ggcttgggtc gacgcttaat
    22201 ggaaaagttg attacctata cgcgagatca cggactacaa cgtctgaatg gtattacgat
    22261 gccaaacaat cgtggcatgg tggcgctggc ccgcaagctc gggtttaacg ttgatatcca
    22321 gctcgaagag gggatcgttg ggcttacgct aaatcttgcc caacgcgagg aatcatgagt
    22381 aaggtactgg aaatgttgac cacttaatcg ggactggtgt tattattgca cgcttatgtc
    22441 gtctgcattg cacagaggac ccttcaatga acagagaaga aatgcactgt gatgttgtca
    22501 aaatttaagc gtaataaaca tcaacaacac cttgcccaac tacccaagat ttctcaatca
    22561 gttgatgatg tcgatttctt ttacgctccc gccgacttcc gggagacgct gctggaaaaa
    22621 atagccagcg cgaagcagcg catttgcatt gtcgccctgt atctcgaaca ggatgacggt
    22681 ggcaaaggca ttctgaacgc gttgtatgaa gctaaaaggc agcgtccgga actggatgtg
    22741 cgggtgctgg tcgactggca tcgtgcacaa cgtggacgca ttggcgctgc ggcatctaac
    22801 actaacgctg actggtactg tcgcatggcg caggaaaatc cgggcgtaga tgttccggtt
    22861 tatggcgttc caatcaatac ccgtgaagcc cttggtgttc tgcactttaa aggctttatc
    22921 atcgacgata gcgtacttta tagcggtgcc agcctgaacg atgtttacct gcatcagcac
    22981 gataaatatc gctacgaccg ttatcatctt atccgtaacc gtaagatgtc agacattatg
    23041 tttgaatggg ttacacagaa tattatgaat ggccgcggcg ttaatcgtct ggatgatgtt
    23101 aatcggccaa aaagcccgga aatcaagaac gatattcgtc tgttccgcca ggagctgcgt
    23161 gatgccgctt atcatttcca gggcgatgcc gacaacgatc agctttccgt aacgccgcta
    23221 gtggggctgg ggaaatcgag tctgttgaac aagaccattt tccatcttat gccttgtgcc
    23281 gagcagaaac taaccatctg tacgccatac ttcaacctgc cagcaatcct tgtgcgcaat
    23341 attatccagt tgctgcgcga agggaaaaag gtcgaaatta ttgttggtga taaaaccgcg
    23401 aacgacttct acattccgga agatgaacct ttcaagataa ttggcgcatt gccttatctc
    23461 tatgagatca atctgcgtcg tttcctgagc cgtttgcagt attacgtcaa tactgaccag
    23521 ctagtggttc ggttatggaa agatgacgac aacacctatc acctaaaagg gatgtgggtt
    23581 gatgataagt ggatgttgat caccggtaat aacctgaacc cgcgcgcctg gcgtctggat
    23641 ctggaaaacg ccattttgat ccacgatcca caactggagc ttgcgccgca gcgagagaaa
    23701 gaactggagc tgatccgcga gcataccacc atcgttaagc actatcgcga tctgcaaagt
    23761 attgccgatt atccggtgaa ggttcgtaaa ctcatccgcc gtttgcgccg tatccgcatc
    23821 gaccgattaa ttagccgcat cctgtaatca caaccccgtc ctgtacgggg tttgtttttt
    23881 ggaggccacg ttttgcgtat tctttttgtc tgttcgctat tgttactttc tggatgcagc
    23941 catatggcta acgatagctg gagcgggcag gataaagctc aacactttat cgcctcggcg
    24001 atgctttccg ccgccggaaa tgaatattca cagcatcagg ggatgagccg ggatcgcagt
    24061 gccatgtttg gtttgatgtt ctctgtcagt ttgggggcgt caaaagagtt ttgggatagc
    24121 cgccccgaag ggagcggctg gagctggaag gatttggcct gggatgtcgc cggtgcaagc
    24181 accggctata ccgtctggca actgacccgt cactaaagac gcatcccctt ccctttgcga
    24241 tgtagcatca aagaaaccag aaacgccacc acggccatca aggtcacata ccagaagaag
    24301 gctgtttcca ttcctattga tttcagcgac aacgctacgt actccgccga accaccaaat
    24361 atagcattag cgaccacata tgacagacca acgcctaatg cgcgaacctg tgccgggaac
    24421 atctcagcct tcagtattcc actgattgat gtataaaaac tcactatcag cagggcacac
    24481 atcaccagac caaaagcggc ataaggcgag gaaacgtttt gcaatgctga gagaatagga
    24541 acggtaaaaa tggctgccag cgaaccgaaa cataacattg aggtacggcg accaatctta
    24601 tccgacagcg cgccaatgag tggttgaata agcatgaata caaacaatgc ggcagtcata
    24661 atgccactcg ccacgttggc atgcattccc gcagtattta ccagatactt ctgcatataa
    24721 gtggtgaagg tatagaaaca aagggagccc gcagcggtaa aaccgagaac catgatgaat
    24781 gcacggcgat tgcgccataa tcctttcaga gatccagctt cttttaaagc gcgcgtttct
    24841 tgttgcgaag tttcatctaa ctgacgacgt aaccacaacg ccacaacagc taacacagct
    24901 cctaacgcga aaggaatacg ccatccccac tctctgagtg cagagtcttc catggtgtgt
    24961 tgtaaaacca cgacaaccag tagggctagc agttgtccgc cgatcaacgt cacatactga
    25021 aatgatgcgt aaaaaccttt gcgcccttca acggcaactt cactcatata ggtggcgctg
    25081 gtgccatatt ctccgccaac ggataatccc tgaaataaac gcgcgagaag caataatgcc
    25141 ggagcccacg taccgattgt ttcataacca ggcaaacagg cgattaccag tgagccgaaa
    25201 cacatcatac acaccgatag cagcatcgat tttttgcgac catgtttatc ggctatgcgg
    25261 ccaaatagcc aaccgcctat tgggcgcatc aggaatcccg cagcaaaaac acctgctgtt
    25321 tgtagtagtt gagtcgtcgt gttcccggaa gggaagaaga tgtgggcaaa gtagagtgaa
    25381 cagaacgagt agacatagaa atcgaaccac tcgaccagat tacctgaaga ggcccccaca
    25441 atcgcccaaa tgcggcgacg agtatcacta cttgtcagtt tgctgtctgc cgttacagta
    25501 ctttcagcca tgccattatg tctcctgccg taatccgatg cttttgtcgg tcgcttttgt
    25561 ttatttcttt tggcaaagaa aatgttatac atttgttacg tatcattatg caaccttaac
    25621 catgaattta gttagcagga aaatggttat tgaggagctt aaggataaat ttctggtaag
    25681 gaggacacgt atggaagtag gcaagttggg gaaaccgtat ccgttgctga atctggcata
    25741 tgtgggagta taagacgcgc agcgtcgcat caggcatttt tttcaccaac gcaaaaaggc
    25801 catcctgacg gatggccttt
//
LOCUS       DAZFAB010000062        25718 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZFAB010000062 DAZFAB010000000
VERSION     DAZFAB010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25718
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.062"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            283..528
                     /locus_tag="ACHJWA_002899"
     CDS             283..528
                     /locus_tag="ACHJWA_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein N"
                     /protein_id="HFO4309459.1"
                     /translation="MCDTKRSIGRKCESGLAANVPLRGVFVQDYDSHTQPKLTNRRIQ
                     MDAQTRRRERRAEKQAQWKADRILASTFRRNATKRHY"
     gene            473..1393
                     /locus_tag="ACHJWA_002900"
                     /pseudo
     CDS             473..1393
                     /locus_tag="ACHJWA_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(1408..1483)
                     /locus_tag="ACHJWA_002901"
     tRNA            complement(1408..1483)
                     /locus_tag="ACHJWA_002901"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1448..1450),aa:Thr,seq:cgt)
     gene            complement(1598..2851)
                     /gene="proA"
                     /locus_tag="ACHJWA_002902"
     CDS             complement(1598..2851)
                     /gene="proA"
                     /locus_tag="ACHJWA_002902"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFO4309460.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVVVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIAYSFLPALSKQ
                     MAESGVTLHADASALAQLQTGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            complement(2863..3966)
                     /gene="proB"
                     /locus_tag="ACHJWA_002903"
     CDS             complement(2863..3966)
                     /gene="proB"
                     /locus_tag="ACHJWA_002903"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFO4309461.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            4254..5309
                     /gene="phoE"
                     /locus_tag="ACHJWA_002904"
     CDS             4254..5309
                     /gene="phoE"
                     /locus_tag="ACHJWA_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFO4309462.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(5348..5749)
                     /gene="crl"
                     /locus_tag="ACHJWA_002905"
     CDS             complement(5348..5749)
                     /gene="crl"
                     /locus_tag="ACHJWA_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFO4309463.1"
                     /translation="MTLPSGHPKSRLIKKFTVLGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5807..7051)
                     /gene="frsA"
                     /locus_tag="ACHJWA_002906"
     CDS             complement(5807..7051)
                     /gene="frsA"
                     /locus_tag="ACHJWA_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO4309464.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSCLLHQRVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            complement(7143..7601)
                     /gene="gpt"
                     /locus_tag="ACHJWA_002907"
     CDS             complement(7143..7601)
                     /gene="gpt"
                     /locus_tag="ACHJWA_002907"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO4309465.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            7862..9319
                     /gene="pepD"
                     /locus_tag="ACHJWA_002908"
     CDS             7862..9319
                     /gene="pepD"
                     /locus_tag="ACHJWA_002908"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO4309466.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(9376..9897)
                     /gene="prfH"
                     /locus_tag="ACHJWA_002909"
                     /pseudo
     CDS             complement(9376..9897)
                     /gene="prfH"
                     /locus_tag="ACHJWA_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(9896..10099)
                     /locus_tag="ACHJWA_002910"
                     /pseudo
     CDS             complement(9896..10099)
                     /locus_tag="ACHJWA_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            complement(10418..10870)
                     /gene="yafP"
                     /locus_tag="ACHJWA_002911"
     CDS             complement(10418..10870)
                     /gene="yafP"
                     /locus_tag="ACHJWA_002911"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO4309467.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFIRAVMMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAQPVGFISRIEHYIDMLFVDPEYTRRGVASALLKPWIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFTNFSMRYKPQH"
     gene            complement(10880..11278)
                     /gene="yafO"
                     /locus_tag="ACHJWA_002912"
     CDS             complement(10880..11278)
                     /gene="yafO"
                     /locus_tag="ACHJWA_002912"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HFO4309468.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDESHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            complement(11281..11574)
                     /gene="yafN"
                     /locus_tag="ACHJWA_002913"
     CDS             complement(11281..11574)
                     /gene="yafN"
                     /locus_tag="ACHJWA_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HFO4309469.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAEKYLNDMTDDDFNDFKE"
     gene            complement(11626..12681)
                     /gene="dinB"
                     /locus_tag="ACHJWA_002914"
     CDS             complement(11626..12681)
                     /gene="dinB"
                     /locus_tag="ACHJWA_002914"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFO4309470.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFSELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVSAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(12752..13537)
                     /gene="lafU"
                     /locus_tag="ACHJWA_002915"
     CDS             complement(12752..13537)
                     /gene="lafU"
                     /locus_tag="ACHJWA_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFO4309471.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            13509..15221
                     /locus_tag="ACHJWA_002916"
                     /pseudo
     CDS             13509..15221
                     /locus_tag="ACHJWA_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(15366..15863)
                     /gene="rayT"
                     /locus_tag="ACHJWA_002917"
                     /pseudo
     CDS             complement(15366..15863)
                     /gene="rayT"
                     /locus_tag="ACHJWA_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414763.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
     gene            complement(16039..16788)
                     /gene="yafL"
                     /locus_tag="ACHJWA_002918"
     CDS             complement(16039..16788)
                     /gene="yafL"
                     /locus_tag="ACHJWA_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO4309472.1"
                     /translation="MSLPSIPSFVLSGLLLICLPFSSFASATTSHISFSYAARQRMQN
                     RARLLKQYQTHLKKQVSYIVEGNAESRRALRQHNREQIKQHPEWFPAPLKASDRRWQA
                     LAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDKGFDCSGLVFYAYNKILE
                     AKLPRTANEMYHYHRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRT
                     GETIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            16998..17258
                     /gene="dinJ"
                     /locus_tag="ACHJWA_002919"
     CDS             16998..17258
                     /gene="dinJ"
                     /locus_tag="ACHJWA_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HFO4309473.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGV"
     gene            17261..17537
                     /gene="yafQ"
                     /locus_tag="ACHJWA_002920"
                     /pseudo
     CDS             17261..17537
                     /gene="yafQ"
                     /locus_tag="ACHJWA_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
     gene            17693..18433
                     /gene="dpaA"
                     /locus_tag="ACHJWA_002921"
     CDS             17693..18433
                     /gene="dpaA"
                     /locus_tag="ACHJWA_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFO4309474.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(18404..19171)
                     /gene="yafJ"
                     /locus_tag="ACHJWA_002922"
     CDS             complement(18404..19171)
                     /gene="yafJ"
                     /locus_tag="ACHJWA_002922"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFO4309475.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGKTDSEKAFCWLLYKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(19276..19854)
                     /gene="lpcA"
                     /locus_tag="ACHJWA_002923"
     CDS             complement(19276..19854)
                     /gene="lpcA"
                     /locus_tag="ACHJWA_002923"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFO4309476.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            20094..22538
                     /gene="fadE"
                     /locus_tag="ACHJWA_002924"
     CDS             20094..22538
                     /gene="fadE"
                     /locus_tag="ACHJWA_002924"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFO4309477.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTLGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNVFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(22581..23027)
                     /gene="ivy"
                     /locus_tag="ACHJWA_002925"
     CDS             complement(22581..23027)
                     /gene="ivy"
                     /locus_tag="ACHJWA_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFO4309478.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            23208..23978
                     /gene="yafV"
                     /locus_tag="ACHJWA_002926"
     CDS             23208..23978
                     /gene="yafV"
                     /locus_tag="ACHJWA_002926"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFO4309479.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMVALREYREKFPAWRDADEFRLR"
     gene            complement(24096..25268)
                     /locus_tag="ACHJWA_002927"
     CDS             complement(24096..25268)
                     /locus_tag="ACHJWA_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc26 family transposase"
                     /protein_id="HFO4309480.1"
                     /translation="MSIQSLLDYISVIPDIRQQGKVKHKLSDILFLTVCAVIAGADEW
                     QEIEDFGHERLEWLKKYGDFDNGIPVDDTIARVVSNIDSLAFEKIFIEWMQECHEITD
                     GEIIAIDGKTIRGSFDKGKRKGAIHMVSAFSNENGVVLGQVKTEAKSNEITAIPELLN
                     LLDLKKNLITIDAMGCQKDIASKIKDKKADYLLAVKGNQGKLHHAFEEKFPVNVFSNY
                     KGDSFSTQEISHGRKETRLHIVSNVTPEFCDFEFEWKGLKKLCVALSFRQKKEDKSAE
                     GVSIRYYISSKDMDAKEFAHAIRAHWLIEHSLHWVLDVKMNEDASRIRRGNAAEIISG
                     IKKMALNLLRDCKDIKGGVKRKRKKVALNTCYIEEVLASCSELGFRTDKMKNLTQI"
     gene            25349..25534
                     /locus_tag="ACHJWA_002928"
     CDS             25349..25534
                     /locus_tag="ACHJWA_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4309481.1"
                     /translation="MIYITIFMILPCPVPCSHVFLYVFYIFLFLVLFIMTIYQSSQKL
                     HFSNCYHNNQHHNSLHN"
     gene            complement(25449..>25718)
                     /gene="ydcD"
                     /locus_tag="ACHJWA_002929"
     CDS             complement(25449..>25718)
                     /gene="ydcD"
                     /locus_tag="ACHJWA_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdcD"
                     /protein_id="HFO4309482.1"
                     /translation="SIMVNNYKTHCGVVDINLNFFNDILYSVRLKNISKLENMEFCAT
                     KQRVYFSDKNKKASYKIINYGDYYDVDYYDNNLKNEVFDWIGKWS"
BASE COUNT         6420 a         6540 c         6343 g         6415 t
ORIGIN      
        1 accaattgta tttattttaa aattaatagg tgcaactcac taaacaacgc aattctgatc
       61 tctcgatcac ctcccaagcc acacaaccct gcaaaaaata aatctatata aaaaacatac
      121 agataaccat ctgcggtgat aaattatctc tggcgatgtt gacacaaata ccactagcgg
      181 tgataccaag cacatcagca ggacgcacta accaccatga aggtgatgct cttaaaaatt
      241 aagccctgaa gaagggcagc attcaaagca gaaggctttg gtgtgtgtga tacgaaacga
      301 agcattggcc ggaagtgcga atccggatta gcagccaatg tgccattgcg gggtgttttc
      361 gttcaggact acgactccca cacacaacca aagctaacta acaggagaat ccagatggat
      421 gcacaaacac gccgccgcga acgtcgcgca gagaaacagg ctcaatggaa agcagatcgg
      481 attctggctt caacatttcg caggaatgca actaagagac attactgaat caaaaattta
      541 ttcagcaatg cagaaaatga cgaaccggcg tcatgaggaa aactggaaac tcagggcaga
      601 agcatgcaga aaaaaaggga aacctgttcc agaatacacg ccaaaaccag cgtccgttgc
      661 aacgaaggct acgcatcttt catttataaa ggccctgcta agagccgcag agcgtgaatg
      721 gaaaatgctc gataaggcac caattattaa agtgcctcaa ccaaagaata aacggatccg
      781 ctggctggag ccccatgaag cacaaaggct gattgatgaa tgtccggagc cattaaagtc
      841 tgttgttgaa tttgcactgg caacaggctt aagacgctcg aacatcatca accttgaatg
      901 gcaacaaata gatatgcagc gccgggtggc atggataaac ccggaagaga gtaaatcaaa
      961 ccgcgcaatt ggcgttgcgc tgaatgatac tgcatgtcgc gtattgaaaa aacaaatcgg
     1021 taatcatcac cgttgggtat ttgtgtacaa ggaaagctgt accaaaccag acggaacgaa
     1081 agcgccaaca gtaaggaaga tgcggtatga cgcaaacaca gcctggaaag cggcgctgag
     1141 acgggctggt attgatgatt tcagatttca cgacttgaga cacacctggg caagttggct
     1201 ggttcaagcc ggagtcccgt tgtcagtgtt acaggaaatg ggaggctggg agtctatcga
     1261 aatggttcgt cgatatgctc accttgcacc taatcacctt accgaacacg cacggcaaat
     1321 agactcgatc ctgaacccat cggtcccaaa tttgtcccag tcaaaaaata aggaaggtac
     1381 taatgatgtg taacttattg atttaaatgg tgccgataat aggagtcgaa cctacgacct
     1441 tcgcattacg aatgcgctgc tctaccaact gagctatatc ggccctgaaa ggacatgttc
     1501 acgaacgtga atcacggtgg acaaggttaa aactaaccgg gcgatgcgtc aatggccttg
     1561 tgaatcaaac ggctactttt gcatcacccg gttttattta cgcacgaatg gtgtaatcac
     1621 caatgccgat ccacttgtaa gtggtcagtg cttccagccc cattgggcca cgcgcgtgga
     1681 gtttttgtgt gcttaccgcc acttccgcac ccagtccaaa ctggccgccg tcggtaaaac
     1741 gcgtagaggc gttaacgtaa acagcggacg aatccacttc gttaacaaaa cgctgggcgt
     1801 tgcgcatatc gcgggtcagg atcgcatcgg agtgttgcgt gccgtgttca cgaatatggg
     1861 cgatggcatc gtcaagatcg ctgacgattt tgacgttcaa atctaatgac agaaactcat
     1921 cgtcatactc ttcggcttta accgccacca ccttcgcagg gcctgtctgc aactgcgcca
     1981 gcgcagatgc atctgcgtgt aacgtcacgc cgctttccgc catttgtttg cttaatgcgg
     2041 gcaggaagct ataggcgatg tttttattca ccagcaacgt ttctaccgta ttacatgtgc
     2101 tcggacgctg agttttcgca ttgacgatta ctttcagggc ttcagcaatc tctgcacttt
     2161 catcaacata aatatggcat acgcctatac cacctgtgat caccgggatc gtcgactgct
     2221 cgcggcacag tttatgcaaa ccagccccgc cgcgcgggat cagcatgtcg atgtatttat
     2281 ccatacgcag catttcactg accagcgcac ggtcaggatt atcaatcgcc tgcacggcac
     2341 ccgccggtaa gccacaggat ttcagggcgt cctgaatcac caccaccgtt gccgcgttag
     2401 tgcgacaggt ttctttgcca ccacgcagga tcaccgcgtt accggttttc aggcacagcg
     2461 aagcgacatc aaccgtcacg ttcgggcgcg cttcataaat cacgccaata acccccagcg
     2521 gtacgcgacg acgctcaaga cgcaggccgc tgtccagtac gccgccatcg attacctgcc
     2581 ccactggatc ggcgaggttg cacacctgac gtacatcgtc ggcaatgcct ttcagccgtg
     2641 cgggcgtcag tgccagacgg tcaagcatcg cttcgctaag gccattggct cgcgcgtcag
     2701 caacatcctg ggcgttagcg ttgaggatga tttcgctttg tgcttccagt tcatcggcga
     2761 ttttttccag cacgcgattt ttttcgcggc tggagagttg cgctaattta tacgaggctt
     2821 gcttcgcggc aatgcccatt tgttccagca tcagcctgct ccttaacggg tgatcatgtc
     2881 atcacggtga acggcaaccg ggccgtattc atatcccagt attgcatcaa tttcttgcga
     2941 gtggtgtccg gcaatacggc gtaatgcatc gctgttgtaa cgactgacgc cgtgggcgat
     3001 atcgcgacct tcgaggttac aaatgcggat gacttcacca cgcgagaagt tgccagtcac
     3061 gcttttaatg cctttcggca acagggagct gccgcgttca agaatggcgg cggttgcccc
     3121 ttcatctacc gtgatttcac ccgcaggcgg cgcaccgaaa atccagcgtt tacggttttc
     3181 aagcggagtc gcctgggcat ggaacagcgt accgacggaa atgccttcca tcacatcacc
     3241 aataacgccc ggcttgctgc ccgcggcaat aatggtgtcg atacccgcac ggcaagccac
     3301 gtcagcggcc tgcaatttgg tactcatgcc gccagttccg aggcctgaaa cgctgtcacc
     3361 agcaatcgcg cgcagtgcgt catcaatgcc gtaaacatct ttaatcagtt ctgcctgcgg
     3421 attgctgcgc gggtcagcgg tatacaaacc tttttgatcg gtcagtaaca acagtttatc
     3481 ggcacccgcc agaatcgccg ccagtgcaga aaggttgtcg ttatcgccga ctttaatctc
     3541 tgccgtagcg acagcatcgt tctcattgat taccggaacg atattgttat cgagcaacgc
     3601 acgcagggtg tcgcgggcgt tcaggaagcg ttcacggtct tccatatcag cacgagtcag
     3661 cagcatttgc ccgacgtgaa tgccataaat cgaaaacagc tgttcccaca gttgaatcag
     3721 tcgactctgc cctaccgccg ccagcagttg tttcgaggcg atagtcgctg gcagttccgg
     3781 gtaacccagg tgctcacgtc cggcggcgat cgcgcccgac gtcacaataa caatccgatg
     3841 cccggcggca tgtaactgcg cgcactggcg aacaagttca acgatatggg cacggttcag
     3901 gcggcgcgat ccgcctgtta gcacactggt gccgagtttt accaccagcg tctggctgtc
     3961 actcatgatt ctctgccatt caattttagg aaaaatgata tcaaacgaac gttttagcag
     4021 gactgtcgtc ggttgccaac catctgcgag caaagcatgg cgttttgttg cgcgggatca
     4081 gcaagcctag cggcagttgt ttacgctttt attacagatt taataaatta ccacatttta
     4141 agaatatcat taatctgtaa tatatcttta acaatctcag gttaaaaact ttcctgtttt
     4201 caacggggct ctcccgcaga atattcgcgc gttaattaaa atcaggaatg aaaatgaaaa
     4261 agagcactct ggcattagtg gtgatgggca ttgtggcatc tgcatccgta caggccgcag
     4321 aaatatataa caaagacggt aataaactgg atgtctatgg caaagttaaa gccatgcatt
     4381 atatgagtga taacgacagt aaagatggcg accagagtta tatccgtttt ggttttaaag
     4441 gcgaaacaca aattaacgat caactgactg gttatggtcg ttgggaagcg gagtttgccg
     4501 gaaataaagc ggagagtgat actgcacagc aaaaaacgcg tctcgctttt gccggattga
     4561 agtataaaga tttgggttct ttcgactatg gccgtaacct gggcgcgttg tatgacgtgg
     4621 aagcctggac cgatatgttc ccggaatttg gtggcgactc ctcggcgcag accgacaact
     4681 ttatgaccaa acgcgccagc ggtctggcga cgtatcggaa caccgacttc ttcggcgtta
     4741 tcgatggcct gaacttaacc ctgcaatatc aagggaaaaa cgaaaaccgc gacgttaaaa
     4801 agcaaaacgg cgatggcttc ggcacgtcat tgacatatga ctttggcggc agcgatttcg
     4861 ccattagtgg ggcctatacc aactcagatc gcaccaacga gcagaacctg caaagccgtg
     4921 gcacaggcaa gcgtgcagaa gcttgggcta caggtctgaa atacgatgcc aataatattt
     4981 atctggcaac tttttattct gaaacacgca aaatgacgcc aataactggc ggctttgcca
     5041 ataagacaca gaactttgaa gcggtcgctc aataccagtt tgactttggt ctgcgtccat
     5101 cgctgggtta tgtcttatcg aaagggaaag atattgaagg tatcggtgat gaagatctgg
     5161 tcaattatat cgatgtcggg gctacatatt atttcaacaa aaatatgtca gcgtttgttg
     5221 attataaaat caaccaactg gatagcgata acaaattgaa tattaataat gatgatattg
     5281 tcgcggttgg catgacctat cagttttaat gattattgcc ggatgtgatg catccggcac
     5341 atttcactca cgccgttaac ttcaccggct cgtcacgaaa atcatccgcc ggttccaact
     5401 tcagattcag cgtcgtcagc agttcacgca gcttctcgtg aaactcacgc agggtgtgct
     5461 ccagtcgttc aaccacttca gtatctttta ccggaacact cttccagtcg cctgctttat
     5521 cgaacagacc aaactggtaa ctgtaggtaa aacgggattc ctgcgcttca agctccatcc
     5581 accagcccca gaactcacgc acttccggtg ccggtttcac gttgacgcat acagccagac
     5641 aatcgaaaaa gaatcgatta tcttcgcact taccttcacg aatatacggg cctagtacgg
     5701 taaatttttt gatcaatctg ctcttcgggt gtccactcgg taacgtcatt gctatctcct
     5761 gttgtgatgc aactgtttta ccaaattggc aaaatttagc aaatttttaa cacaagcgtt
     5821 tttcgatcca gtcggtgatt tcctgaagac ctttgtcaaa attccgatac accgggttaa
     5881 atgggatctc taataattta ccgtcagcag atgatgaggt gattaagcgt gagtcctctt
     5941 ccgggctgaa cggatcgttc ttccagtagc ctgataacat tggcgttggg catcgacgtc
     6001 ccagcaatcc ttgcactttt aatgaatagc gattcagctc cacgcgcaac gcttcgtcgg
     6061 aagcatcatg catccccaaa cgactcgcca gaacgtcaag atacatttcc ggcacctgtt
     6121 gctggcactt aaaatcactc aacagggtat gaactaccgg accaagacag gcaaccgctt
     6181 tcagacgcgg cgattcaaga tatgccagac gcacggcaac gttagcgccg aaacggaaac
     6241 caaaggccgc gacgcgagtg tgatccaccc acggtacgtt aggcagagcc tttaagacgc
     6301 gctgatgcaa caggcaggag tcctgggtga gcttccattt tgaagaaaag cccaccgacg
     6361 gcatatcaat agtcagcatc gcaatgccgc gcggcgcaaa ataacgttca tacaggctgt
     6421 aatagtccgt ctgcatcgca tccagaccac cacacattaa taccgtcggg aacgggccat
     6481 cgcctttcgg catatgcaaa aagccggtga tgggcgcacc gccgggtacg gtaaactcca
     6541 tctgtcgcat cgtgcccggt agacgctgag cggcctcttc ataggcgcgg tttgacaaag
     6601 cctgcgcttg ctcggccagg tcatctcctt tcagatgagg ataggcggca atgttgtaca
     6661 acgtagccgc atgcagccag tgacgaccac tgagttgcgg atcttcttcc gcacaggctt
     6721 tttgttgcca caccatcgcc tgggtggccc actcataaat ccagttgccg ccacggtagc
     6781 caatcaccgt atcgtataaa tcatcgtcgg tacgttcggc atcgctcatc acaatacgtg
     6841 cctggacgtc gaggatttcg cgtgggtcaa tgccgcgcca gatccacatc agacggttaa
     6901 tcatgcgata ccagtgaggg atggttttac catcaagggc cgactgcaca ggcggttgtg
     6961 cgccgtgatt aaagcggcgg actagcgtcg aggtttcagg atgtttaaag cggggtttga
     7021 acagggtttc gctcaggttt gcctgtgtca tggatgcagc ctccagaata cttactggaa
     7081 actattgtaa ctcgcctgaa gttaaaaaga acaacgcccg gcagtgccag gcgttgaaaa
     7141 gattagcgac cggagattgg cgggacgaat acgacgccca tatcccacgg ctgttcgatc
     7201 caggtatctt gcgggatatc aacaacataa tcatcaacca gcggacgacc agccggtttt
     7261 gcaaagatgg tgacaaagtg cgcttttgga tacatttcac gaatcgcaac cgcagtacca
     7321 ccggtatcca ccaggtcatc aataacgatg aagccttcgc catcgccttc tgcgcgtttc
     7381 agcactttaa gctcgcgctg gttgtcgtga tcgtagctgg aaatacaaac ggtatcgaca
     7441 tgacgaatac ccagttcacg cgccagtaac gcacccggta ccagaccgcc acggcttacg
     7501 gcaataatgc ctttccattg ttcagaaggc atcagtcggc ttgcgagttt acgtgcatgg
     7561 atctgcaaca tgtcccaggt gacgatgtat ttttcgctca tgtgaagtgt cccagcctgt
     7621 ttatctacgg cttaaaaagt gttcgagggg aaaataggtt gcgcgagatt atagagatct
     7681 ggcgcactaa aaaccagtat ttcacatgag tccgcgtctt tttacgcact gcctctccct
     7741 gacgcgggat aaagtggtat tctcaaacat atctcgcaag cctgtcttgt gttgacaaca
     7801 ttttctgcta accctgtgac ctgcaatact gttttgcggg tgatcgacaa ggagacttaa
     7861 cgtgtctgaa ctgtctcaat tatctccaca gccgctgtgg gatatttttg ccaaaatctg
     7921 ttctattcct cacccgtcct atcatgaaga gcaactcgct gaatacattg ttggttgggc
     7981 aaaagagaaa ggtttccatg tcgaacgcga tcaggtaggt aatatcctga ttcgtaaacc
     8041 tgctaccgca ggtatggaaa atcgtaaacc ggtcgtcttg caggcccacc tcgatatggt
     8101 gccgcagaaa aataacgaca ccgtgcatga cttcacgaaa gatcctatcc agccatatat
     8161 tgatggcgaa tgggttaaag cgcgcggcac cacgctgggt gcggataacg gcattggtat
     8221 ggcctctgca ctggcggttc tggctgacga aaacgtggtt cacggcccgc tggaagtgct
     8281 gctgaccatg accgaagaag ccggtatgga cggtgcgttc ggcttacaga gcaactggtt
     8341 gcaggctgat attctgatta acaccgactc cgaagaagaa ggtgaaatct acatgggttg
     8401 cgcggggggt atcgacttca cctccaacct gcatttagat cgtgaagcgg ttccagctgg
     8461 ttttgaaacc ttcaagttaa ccttaaaagg tctgaaaggc ggtcactccg gcggggaaat
     8521 ccacgttggc ctgggtaatg ccaacaaact gctggtgcgc ttcctggcgg gtcatgcaga
     8581 agaactggac ctgcgtctta ttgatttcaa cggcggcaca ctgcgtaacg ccatcccgcg
     8641 tgaagccttt gcgaccattg ctgtcgcagc tgataaagtc gacgccctga aatctctggt
     8701 gaatacctat caggagatcc tgaaaaacga gctggcagag aaagagaaaa atctggcctt
     8761 gttgctggac tctgtagcga acgataaagc tgccctgatt gcgaaatctc gcgatacctt
     8821 tattcgtctg ctgaacgcca ccccgaacgg tgtgatccgc aactccgacg tggcaaaagg
     8881 tgtggtcgaa acctccctga acgtcggtgt ggtgaccatg actgacaata acgtagaaat
     8941 tcactgtctg atccgttctc tgatcgacag cggtaaagac tacgtggtga gcatgctgga
     9001 ttcgctgggt aaactggctg gcgcgaaaac cgaagcgaaa ggcgcatatc ctggctggca
     9061 gccggacgct aattctccgg tgatgcatct ggtacgtgaa acctatcagc gcctgttcaa
     9121 caagacgccg aacatccaga ttatccacgc gggcctggaa tgtggtctgt tcaaaaaacc
     9181 gtatccggaa atggacatgg tttctatcgg gccaacaatc accggtccac actctccgga
     9241 tgagcaagtt cacatcgaaa gcgtaggtca ttactggaca ctgctgactg aactgctgaa
     9301 agaaattccg gcgaagtaat tatttgattt gctgccggat ggcgtttaat cgccttccgg
     9361 cagtttcatc cttcattatc cttcgataaa agccatccct gtgaacgttc gtcgcgggtt
     9421 gccacgttca atctggtgat ggaacattcg ccgctgcgat ttcagcactg cgctattttc
     9481 ctgttgctgc tgctccagct tccaggcaat cagcaatcgc gccagccgtt tgttagcatg
     9541 ctggctgcgt tctgactgaa ccttcacgct aatcccggtt gccagatgcg tggcgcgtac
     9601 cgccgagtcg gttttattga catgttgacc gcccggcccc gacggacgca gcgtctcata
     9661 acggattgca tccgattgtt cctgctcatc agcggtaaaa cgcccaatgc ccagaaacca
     9721 gtttttgcgc ccatgatgag gccgatacgg actcagacaa atccactgaa tagtgccgca
     9781 ccaactttcg cttaacgccc atgcgttgtc gccatccaga gaaatcagcg ccgaacgcag
     9841 cgtgtcagag tagcggcccg tttctgtttc cagcaccgtt accgcgacgt cttgtcgggt
     9901 aaaaacgacc aacagagaag aacgctgcgc caatcgggta gccgcgtccg gggtgtaaat
     9961 ctggcatccc cactacgcgc tgcatgttgg gtaaatttgc cgtggtgtgt aattgttgga
    10021 tcgctaaact ctcgatccac aggtcatcag atgcgatggt aaataccgca tcggataagg
    10081 gacgaatata tttgcccatg taccattcca tttatactcg tcatacttca agttgcatgt
    10141 actgcgactg cgttcgttca ccccagtcac ttacttatgt aagctcctgg ggattcactc
    10201 gcttgtcgcc ttcctgcaac tcgaattatt tagagtatat aaatataaaa atcaggaaat
    10261 ggcagacgaa tccagggcaa gaaaaaaaag aattaatcct ggaaaggtct gcaaagagtc
    10321 attgataaag gatttgtcat gatttacctc cttgcagttt gtggaataag aaaacgcgca
    10381 cataccaggc gggcgttatt ttcattgcaa gctggattta atgttgcggt ttatatcgca
    10441 tagaaaaatt agtaaaccac gctccccggc attcaacgcg ctgctgctta actgtctgaa
    10501 aaccataacg ttcaaaaaag ggttttgcgg ttatgctggc gtccaccgta agttcggatt
    10561 cagacttaat ccaaggtttt aacaaagcgc tggcaacccc acggcgggtg tattcagggt
    10621 caacaaataa catatcgata taatgttcaa tgcgggaaat aaaaccaacc ggttgtgcat
    10681 taatgactgc aacccgcact tgtgatttcg cgagtttctc cttccagcga gattcgtcaa
    10741 tctgcgccca ggcggcaatt tgttgtggtg aataatgctg actggcggtc atcataaccg
    10801 ctctaatgaa aatagcgcat agttgctgaa aatcgccagg ctgatagttt cttatttgta
    10861 tgttattcat aatataaatt caaaaacgca tgcgaaacgc ttctgccatt ttcccaattt
    10921 tatgcatttg gttgttatct cgagccagtt tatgaggttc aggtttcaga atggcaatga
    10981 gcaaccatgc ttgctcatca aacgcaccct gacaatatac caaatgcgat tcgtcattcg
    11041 ttctgctgaa ttggcgcaac tgtggcggaa atggattatt cacatttgcc agatgaatat
    11101 gagcaactcg ctcaaatttg attaatggcc aagtaaacga gtcgtcgtag agtgcatcgc
    11161 gaccaaatat atctggcaaa acaccgtcac gcttatagga aataaaatcc gccgttaacg
    11221 catcaagctc ctctgctgta agttgcaggc gaataagttt tgttttgaat acccgcatcc
    11281 ttattcctta aagtcattga aatcatcatc cgtcatatca ttaagatatt tttcagctcg
    11341 gcgtgtaatt tcctctaatc ttgcagcact tggacggaag cgcgccttta tgggcttttt
    11401 ctcctcttgt tcagcaagca tgtccattaa cgcttcgaat gcgctggcac ttaagagata
    11461 tcctgcgggg cgattattag aaagaaccgc aaccggttga tcaataaagt atttagctgg
    11521 gtttttacgt aattcagtga tattgaccga tttttcagcg agaattcgat gcatacagtg
    11581 atcccctcat aataatgcac accagaatat acataatagt atacatcata atcccagcac
    11641 cagttgtctt tccatttgag ggtcaagcaa cgtcacatgc agccccacca gtcgcacacc
    11701 gcgcccgccg cggcgttcat cccaggtttt acgcgcggtg gcgattaagt cagctttatt
    11761 cagccgcggc cagacgtgct cctgggtggt ttgctgaaaa tcgtcgaact ttaatttcac
    11821 cccctggcga gcaatcagta aatcaggttt tacctttgcc agacggcgtt caagttccgg
    11881 atacagccgc tcgataatcg cttcacattc agaccagtga tgaatatctt ccgccatcgt
    11941 gcgttccacg ccgacggatt ttcgtaaccg ttcgctgtta acgtcgcgct cgtcaatccc
    12001 ctgactacgc tcccacaaaa tgcggccaaa tttgccaaag cgtttaagca gaatcaccag
    12061 atcacacttt tgtacatcac cgcaggtccg cagccccatc gcttccagct ttgccgctga
    12121 gactttgcca acaccgggaa tttttgccag cggtaaggtt tgcagaaatg ccgaaacttc
    12181 tgccggcgta atcacaaact ggccgttggg tttattcatg tcggaggcga ttttggcgag
    12241 aaactttact ggtgccacgc ccgcagacgc cgtcagttgc agctcgctga aaattgtctg
    12301 gcggatttcc tgggcgatga gggtcgcaga accgtggcaa tggacgctat cagtgacatc
    12361 gaggtaggct tcatccagtg acaacggttc aatacgcgag gtgtagcgcg agaagatttc
    12421 acggatatga tttgaggctt ctttgtaggc gtcaaagcgc cccggaagca aggtgagatg
    12481 tgggcataat ttgagcgcca tccctgtcgg catagcgctg cgtacgccaa atttacgcgc
    12541 gggataattg gcggtgctga tcaccccccg acgttcgcgg ctgccgccaa tagcaatagg
    12601 gatatcgcgc agggcgggat tgtcgcgcat ctccactgcg gcgaaaaagc agtccatatc
    12661 cacatgaatg attttacgca ttactcacct ctcaacactg gtaaagtata cagtgatttc
    12721 agggtttgag aaatgcgtaa agattcagca tttagcgcgt tcgctgcgaa agcacctgct
    12781 gcttatccag cttttgcacc agcggctgca ccactttatc cccatgctga ccaaagtatt
    12841 gatacagcgt atcggacgca cttttggtca gcaccataat ctcaatgcgt cggttgcccg
    12901 cgctttgcgg atttttcgcg tccagcagca tctggtccgc cattgcgctt acctgcatca
    12961 ctttatcttc cggcattccg gcctcttcca gcacccgacg agctgaaagc gcgcgatcgc
    13021 ccgaaaggtt ccagttgttg tagatattgt ttttgtaggc catcgcatcg gtatgcccgg
    13081 taataataat tttgttatcg agcgagtcga acactggcgc gagctccacc agcaaagttt
    13141 taaagaacgg cataatctgc gcgctgccgc gttcgaacat attgcgattc tggtcgtctt
    13201 taatcagcac gcgtaatccc tgcgggacaa tctccatctc cagattcgct tccatatgcg
    13261 catcacgggc aatggtgttg atactggttg ccagctcgcc aagctcggtg gcggatttct
    13321 tcttcagcag cgccgttttc tcattcacat cacgcgcttt tttctccgtc tcttccggca
    13381 ccgccacggt ggcaggtttt ggcggatgag acgggctgat tttatttaac ggcgacagcc
    13441 caccaccgtt aaaaatcgac tgcccgtgca gcgcggcgat aatgctttca cgctcggatt
    13501 tactgacgct gttgacaatc aactttatcg tcgtcaccaa aggggccgag cgtatttccg
    13561 aggtttctgc ccgctttacc ctggatgcga tgcccggcaa acagatggcg attgacgccg
    13621 atcttaacgc cggattgatc aaccaggcac aggcgcaaac ccggcgtaaa gatgttgcca
    13681 gcgaggccga tttctacggc gcgatggacg gggcatcgaa gtttgtgcgc ggggatgcca
    13741 tcgccgggat gatgattctg gcgattaacc tgatcggcgg cgtctgtatc gggatattta
    13801 aatacaacct gagcgccgac gccgccttcc agcaatatgt gctgatgacc atcggcgatg
    13861 ggctggtggc gcagatccct tccctcctgc tctccaccgc agcggcgatt atcgtcaccc
    13921 gcgtcagcga caacggcgat atcgcccatg acgtgcgcca ccagctgctg gcaagcccgt
    13981 cggtgctcta caccgccacc gggattatgt ttgtgctggc ggtggtgccg ggaatgccgc
    14041 acctgccgtt tttgctgttc agcgccctgc tgggttttac cggctggcgg atgagcaaac
    14101 agccgcaggc ggcggaggcg gaagagaaaa gcctcgaaac gctgacccgc accatcactg
    14161 aaaccagcga gcaacaggtc agttgggaaa ccattccgct tatcgagcct atcagtttaa
    14221 gcctcggcta caagctggtg gcgctggtcg ataaagcgca gggcaacccg ctcacccagc
    14281 gtattcgcgg cgtacggcag gtgatatccg acggtaacgg cgtgctgctg ccggagatcc
    14341 gcattcggga aaacttccgc ctcaagccca gccagtacgc cattttcatc aacggcatta
    14401 aggccgatga agcggatatt ccggcggata aactgatggc gctgccctcc agcgaaacct
    14461 acggcgagat tgacggcgtg ctggggaacg acccggcgta cgggatgccg gtgacctgga
    14521 tccagcccgc gcagaaggcg aaggcgctga atatggggta tcaggtgatc gacagcgcca
    14581 gcgtgattgc cacgcatgtg aacaagattg tgcgcagcta tattcctgat ttgtttaatt
    14641 atgatgacat cacgcagctg cataaccgcc tgtcgtcgat ggccccgcgc ctggcggaag
    14701 atttgagcgc ggcgctgaat tacagccagt tgctgaaagt gtaccgtgcg ctgctgaccg
    14761 aaggcgtttc cctgcgcgat atcgtcacca tcgccaccgt gctggtcgcc agtagcgcgg
    14821 tgactaaaga tcatattctg ctggcggccg atgtgcgcct ggcgctgcgg cgcagcatta
    14881 cccatccgtt cgttcgcaag caggagctga cggtgtatac gctgaataat gagctggaaa
    14941 atctgctgac taacgtagtg aatcaggcgc aacagggcgg taaagtgatg ctcgacagcg
    15001 tgccagtgga cccgaatatg ctcaaccagt tccagagcac gatgccgcag gtgaaagagc
    15061 agatgaaagc ggcggggaaa gacccggtgc tgctggtgcc gccgcagctg cgccctttgc
    15121 tggcgcgcta tgcaaggttg tttgcgccgg ggctgcatgt gctgtcgtat aacgaagtgc
    15181 cggatgagct ggagttgaag ataatggggg cgttgagttg attggccttt aaggggcgca
    15241 tccggcattg gtgcacaatt gccggatgcg gcgtgaacgc cttatccggc ctacggttga
    15301 gtgcagtttt gtaggcatga taagacgcgc cagcgtcgca tcaggcatcc ggcaccagcg
    15361 cacaattatg aaatgatacg ctccccggca ctaaaatccg ttatgtctcc cgcccaatca
    15421 atgggatata accctcgcgc cacatcgcga tggaacgttg agaatggcca atcactcact
    15481 tgctttaccc agccatgttt tactggattt atataaatat aatcaacatg atggcgatag
    15541 tctttggtat tgcggatggt gtgctcctaa aaacgtggtt gccagatatt tttcaatcca
    15601 caagcatggg tgaactgctt tttaatctcc cgccagcggg aggaaaaatc atcatcgctt
    15661 tcgggtaatg tccatatgca atgcatatgc tccggcaaaa cgacccaggc gttaatttca
    15721 aaaggtctgt ctcgcttaac gttaataatg gcgttacgga gcatctggaa ctgagcggtc
    15781 agaaggtggc ttcgacgatt tcgtaggtta accgtgaaaa accaggttcc ccctttgatg
    15841 taataccgac gatattcaga catggcaact cctttttctc aacggtaaga agaaaaggaa
    15901 aggtcggcaa tgaggtgatg gaataacggg aaagcggctc gcaaaaacgc acagatttgt
    15961 aggcatgata agacgcgtca gcgtcgtatc aggcatctgc gcacaactgc cggatgcggc
    16021 gtgaatgcct tatccgtcct acaaaatcgt ctcttccgtc aaaatccggc gcgcgcctaa
    16081 aaagtgatcc tgccagaaag gttcagctaa ccgacttacc cgaatggttt ccccggtgcg
    16141 cggtgactca ataaactgac cgtcgcccag atacaccccc atatggtcgg caatctcgcg
    16201 gctgtggata tggaaaaaca gcaaatctcc ccggcgcagg tcgttattcg ccacaatcgt
    16261 tgcccgatgg tagtgataca tctcattagc cgtacgcggg agcttcgcct cgaggatctt
    16321 gttgtaggca taaaaaacca acccgctaca gtcaaagcct ttatcaggcc gcgtgccgcc
    16381 ccagacgtaa ggcttgccaa gctgctgctc caggcggtga atcgccactt cggtaatgtt
    16441 atgcagatgg tcgctgctta aaaagtggtt gttttccgcc agcgcctgcc agcgtctgtc
    16501 actcgccttg agcggtgcag gaaaccattc tggatgctgt tttatctgtt cgcggttgtg
    16561 ctggcgtagc gcccttctgc tttcggcatt gccttccaca atatagctga cctgcttttt
    16621 cagatgggtt tggtactgtt ttaataaacg cgcacggttt tgcatccgct ggcgggcggc
    16681 gtagctgaaa gagatatgtg atgtggtggc gctggcaaat gaagaaaacg gcaaacaaat
    16741 caacagtaat cccgacaata caaaggatgg aatcgacggc aaggacatac tcaataatgg
    16801 ctggtcatgc attgatgtta cagtgtatcc cggcccgtac ctgaacaaac ttaataaacc
    16861 attattataa aattaataaa tatatttcag ttaattatgt aaaaacatcc gtttattaaa
    16921 ttactcatag attgtcgctg ttgctcattt gagctacaat tcaagctgaa taaatataca
    16981 gcataggaga tatcccaatg gctgctaacg catttgttcg cgcccgaatc gatgaagatc
    17041 tgaagaatca ggcggcggac gtactggccg ggatggggct gaccatctct gacctggttc
    17101 gcataaccct cacaaaggtc gcgcgtgaaa aggcattgcc gtttgattta cgcgagccta
    17161 atcaattaac cattcaatca atcaaaaaca gcgaagctgg cgttgatgtt cataaggcca
    17221 aagacgccga tgatttattt gataaattag gagtttaaat atgattcaaa gggatattga
    17281 actcgggaca attttcaaag gatgtaaaac ttgcacaaaa gcgtcataag gatatgaata
    17341 aattgaaata tcttatgacg cttcttatca ataatacttt accgcttcca gctgtttata
    17401 aagaccaccc gctgcaaggt tcatggaaag gttatcgcga tgctcatgtc gaaccggact
    17461 ggatcctgat ttacaaactt accgataaac ttttacgatt tgagagaact ggaactcacg
    17521 cggcgctctt tgggtaaagc ccgctgccga tgaaatttgt aaaccctgcg ccgacaggct
    17581 tgcgtaaaac tcttttggtg ataatgtgag ccttcccaat ccagactatt ccgatttggg
    17641 atccattttg ccgtcctggc gtgtgtaacc gttttatcaa ggaataagca gtatgcgtaa
    17701 aatcgcatta attcttgcga tgttgttgat cccgtgcgtt tcgtttgccg gtttgctggg
    17761 cagcagtagc tcgaccacgc cagtgagcaa agagtataag cagcagttga tgggatcccc
    17821 tgtctacatc cagatcttca aggaagaacg tacgctcgat ctctacgtca aaatgggcga
    17881 gcaatatcaa ctgctcgaca gctataaaat ctgtaaatat tccggcggct tagggccaaa
    17941 acagcgtcag ggcgatttca aaagcccgga agggttttat agcgtccagc gtaatcagtt
    18001 aaaaccagac agccgttact acaaagcgat taatattggt ttccccaatg cctatgaccg
    18061 tgcgcatggt tatgaaggga aatacctgat gatccacggc gattgtgttt ccatcggctg
    18121 ctacgcgatg accaatcagg gcattgacga gattttccag ttcgttactg gcgcactggt
    18181 atttggtcag ccgagcgtgc aagtgagcat ttacccgttc cgcatgaccg acgccaatat
    18241 gaagcgccat aaatattcca actttaagga cttctgggag caactgaagc caggctacga
    18301 ctactttgag caaacccgta agccaccgac cgtttctgtg gtcaatggcc gttacgtggt
    18361 cagcaagccg ttaagccacg aagttgtgca accacaactg gcatcaaact acacgctccc
    18421 cgaggcaaaa taagcgccat tcgcctggca taatcttttg ccaggtttca ttgcccgtca
    18481 gcggctgtgt cgcaatcacc gtgaccacat cattcggtgt ggtctgcgag ctgaagtcga
    18541 tttccacatc ctgatccagc aacgttgcca cgccaaacgg cgcgcggcgg gtgatccagt
    18601 gcagattggt cgagcaatac gccattacat agcgcccatc cgaaagcagc atgttgaaaa
    18661 cgcccttctg ccgcagttca tccgccagtg aggcgatata tttaaatacc gccgccatat
    18721 tgcccggcgt acgcggataa cgctgcgtta atttatacag cagccagcaa aaggcttttt
    18781 cgctgtcggt tttacctacc gggcggaagt tgccggtttc cagtgattta tagcccgtca
    18841 gttgtccgtt atgggcataa gtccagttac gtccccataa ctcgcgggta aacgggtgag
    18901 tattttccaa cgccacctcg ccccgatttg cctggcgaat atgagccaca accgaacagg
    18961 attttatcgg gtagtcctga acaagtttgg caatggggga attaaagctg ggttgtggat
    19021 ctttaaatgt gcgacagcct ttaccttcgt aaaaggtaat gccccagcca tctttatgtg
    19081 gcccggttcc accaccacgc tgcaccagcc cggtgaaact aaagcaaata tcggtaggga
    19141 cgttggcgct catcccgagc aattcgcaca tcggtaaccc ccacaacagc gggagcgcat
    19201 ggcccccggc gaagattgac ttaagaattt tcaggccgga tgcggcgtaa acgcattatt
    19261 cggcctacgc cagacttact taaccatctc tttttcaatt aactggatca ggatatggat
    19321 cactttaatg tgaatttcct gaatgcggtc ggcataacca aaatgtggaa cgcgaatttc
    19381 aatatccgcc gtaccagcca ttttgccgcc atctttgccg gtcagggtaa tcactttcat
    19441 tcccttctca cgcgccgccg cgatcgcttt gatcacgttt gcagagttac cagacgtgga
    19501 gatccccagc agtacatcgc cttcgcggcc taccgcttca acatagcggg agaaaatatc
    19561 attgaaacca aaatcgttac cgacgcagga aatatggctg acgtcagaga tggcaatcgc
    19621 cgggtagccc ggacggtttt cacggtagcg accggtcagc tcttcggcaa agtgcatggc
    19681 gtcgcaatgg gagccgccgt tgccgcagga aagcaccttg ccaccggctt taaagctgtc
    19741 tgctaacagg accgccgctc gctgaatggc gtgaatattg gcgtcatctt ttaaaaagtt
    19801 agccagcgtt tccgccgctt cgttcagttc gttacgaata agatcctggt acatgaggat
    19861 atccttcagc ataaatgtaa tagacaaaat gcagtgtacc ggataccgcc aaaagcgaga
    19921 agtacgggca ggtgctatga ccaggacttt ttgccctgaa gtgtagataa aaacagcaac
    19981 aatgtgagct tcgttgtaat tatattgtaa acacattgct aaatgttttt acatccacta
    20041 caaccatatc atcacaagtg gtcagacctc ctacaagtaa ggggcttttc gttatgatga
    20101 ttttgagtat tctcgctaca gttgtcctgc tcggcgcgtt gttctatcac cgcgtgagct
    20161 tatttatcag cagtctgatt ttgctcgcct ggacagctgc cctcggcgtc gctggtctgt
    20221 ggtcggcgtg ggtactggtg cctctggcca ttatcctcgt gccgtttaac tttgcaccta
    20281 tgcgtaagtc gatgatttcc gcgccggtat ttcgcggttt ccgtaaggtg atgccgccga
    20341 tgtcgcgcac tgagaaagaa gcgattgatg cgggtaccac ctggtgggaa ggcgacttgt
    20401 tccagggcaa gccggactgg aaaaagctgc ataactatcc gcagccgcgc ctgaccgcag
    20461 aagagcaagc gtttctcgac ggcccggtag aagaagcctg ccggatggcg aatgatttcc
    20521 agatcaccca tgaactggcg gatctgccgc cggagttgtg ggcgtatctg aaagagcatc
    20581 gcttcttcgc gatgatcatc aaaaaagagt acggcggcct ggagttttca gcttatgccc
    20641 agtctcgtgt gctgcaaaaa ctctccggcg tcagcgggat cctggcgatt accgtcggcg
    20701 tgccgaactc attaggcccc ggcgagctgc tgcaacatta cggcactgac gagcagaaag
    20761 atcactatct gccgcgtctg gcgcgtggtc aggagatccc ttgctttgca ctgaccagcc
    20821 cggaagcggg ttccgatgcg ggcgcgattc cggacaccgg gattgtctgc atgggcgaat
    20881 ggcagggcca gcaggtgctg gggatgcgtc tgacctggaa caaacgctac attacgctgg
    20941 caccgattgc gactgtgctt gggctggcgt ttaaactctc cgacccggag aaattactcg
    21001 gcggtgcaga agatttaggt attacctgtg cgctgatccc gaccaccacg ccgggcgtgg
    21061 aaattggtcg tcgccacttc ccgctgaatg tgccgttcca gaacggaccg acgctcggta
    21121 aagatgtctt cgtaccgatc gactacatta tcggcgggcc gaaaatggcc gggcaaggct
    21181 ggcggatgct ggtggagtgc ctctcggtag gccgcggcat caccctgcct tccaactcaa
    21241 ccggcggcgt gaaatcggta gcgctggcaa cgggcgcgta tgctcacatt cgccgtcagt
    21301 tcaaaatctc tatcggtaag atggaaggga ttgaagagcc gctggcgcgt attgccggga
    21361 acgcttatgt aatggacgct gcggcatcgc tgattaccta cggcattatg ctcggcgaaa
    21421 aacctgccgt gctgtcggct atcgttaagt atcactgtac ccaccgcggg cagcagtcga
    21481 ttattgatgc gatggatatt accggcggta aaggcattat gctcgggcaa agcaacttcc
    21541 tggcgcgtgc ttaccagggc gcaccgattg ccatcaccgt tgaaggggct aacattctga
    21601 cccgcagcat gatgatcttc ggacaaggag cgattcgttg ccatccgtac gtgctggaag
    21661 agatggaagc ggcgaagaac aatgacgtca acgtgttcga taaactgttg ttcaaacata
    21721 tcggtcacgt cggtagcaac aaagttcgca gcttctggct gggcctgacg cgcggtttaa
    21781 ccagcagcac gccaaccggc gatgccacta aacgctacta tcagcacctg aaccgcctga
    21841 gcgccaacct cgccctgctt tctgatgtct cgatggcagt gctgggcggc agcctgaaac
    21901 gtcgtgagcg tatctcggca cgtctgggtg atattttaag ccagctttac ctcgcctctg
    21961 ccgttctgaa gcgttatgac gacgaaggcc gtaatgaagc cgatctgccg ctggtgcact
    22021 ggggcgtaca ggatgcgctg tatcaggcag aacaggcgat ggacgattta ctgcaaaact
    22081 tcccgaaccg cgtggttgcc gggctgctga atgtggtgat cttcccgacc ggacgtcatt
    22141 atctggcacc gtctgacaag ctggatcata aagtggcgaa gattttacaa gtgccgaacg
    22201 ccactcgctc acgtattggt cgcggtcagt acctgacgcc aagcgagcat aacccggttg
    22261 gtttactgga agaggcgttg gtggatgtga ttgccgccga cccgattcat cagcggatct
    22321 gtaaagagct cggtaaaaat ctgccattta cccgtctgga tgaactggcg cacaacgcgc
    22381 tggcgaaggg gctgattgat aaagatgaag ccgctattct ggtgaaagcg gaagaaagcc
    22441 gtctgcgcag tattaacgtt gatgactttg atccggaaga gctggcgacg aagccggtaa
    22501 agttgccgga gaaagtgcgg aaagttgaag ccgcgtaatt gaataacgga gccgaaaggc
    22561 tccgtttctt tatccgctaa ttatttaaaa ttaaagccat ccggatggtt ttccaggctg
    22621 ccggtcaacg ccgcgaacag caccgtttta ccatcaatcg aaagcgcatc gttcacattc
    22681 aaccaggtca gtttctcttg cgacgttttc tcatcaatag tcgagaacag ccccgtcatc
    22741 tgattagatt tctcggacca catcacagcg atacgttgcg agccacagtc atgcggtttg
    22801 cacgcgctca tcacctgata cgtctcatct cccaacgtta cggtttgtgc aggggtataa
    22861 gtaccgcctt tcatcaccca ggcaggcagc ttatgccctt gcaccatctg attaaatgca
    22921 gctttggtgg tttcgccctt tgcaaggctg ctaatggtta aatcatcctg cgccattgca
    22981 ctggtggcga tgaccagtgc ggcgactgtc gttattgcct taaacatcat tcctcccgag
    23041 cttatcctgc ccatgttatt aacctcctaa agcctggcga taacacgata atttgcaaat
    23101 cagatatttc caatatcagc atttgttatc aatttattct gcgctaaggc aactgcctga
    23161 taaggtgtta aagttataaa acccttcgca taaaggagtt gtacaccgtg cctggtttga
    23221 agattacgct tttgcagcaa ccactggtgt ggatggatgg tcctgccaac ctgcgtcatt
    23281 ttgatcgtca actggaaggt attaccgggc gcgatgtgat cgttctaccg gagatgttta
    23341 ccagcggctt tgccatggaa gcggctgctt cgtcgctagc acaagatgac gtagtgaact
    23401 ggatgacagc caaagcgcag cagtgcaatg cgctgattgc aggcagtgtt gcattacaaa
    23461 cggagtctgg ttcggttaac cgctttttgc tggttgagcc gggcggcacg gtacattttt
    23521 atgataagcg tcatctgttc cgcatggcag atgagcatct gcattataaa gcgggcaatg
    23581 cgcgagtgat tgtggaatgg cgcggctggc gtattttgcc gttggtgtgc tacgacttac
    23641 gttttccggt gtggtcgcgc aatctcaacg attatgacct cgccatctac gttgccaact
    23701 ggcctgctcc gcgctctctg cactggcagg cattgctgac ggcccgcgcg attgagaatc
    23761 aggcgtatgt ggcgggatgc aatcgcgtcg gcagcgatgg caacggctgc cattatcgcg
    23821 gtgacagccg ggtgattaat ccacaaggcg agattatcgc tactgccgac gcgcatcagg
    23881 caacgcgcat tgatgcggag ctgtcgatgg tggcattgcg ggaatataga gaaaagtttc
    23941 cggcatggcg ggatgcggat gagtttaggt tgcggtgagt gggggctgta tttagggcgg
    24001 ggaattcaga aagggatgtt tagtgtgctg agcgagagtt aaataataaa aaactgggaa
    24061 gatttaataa tccccccgca gggcaagagc atgaattaaa tctgtgttaa atttttcatt
    24121 ttgtcagttc gaaacccaag ctctgagcag gatgcaagca cttcttctat ataacatgtg
    24181 tttaacgcaa ccttctttct tttcctcttc accccaccct taatgtcttt gcaatctctt
    24241 aataaattca gtgccatctt ctttattcca gatattattt cggctgcgtt tcctcttctt
    24301 atccggctgg catcttcatt catttttaca tctaacaccc aatgaagact gtgctcgatc
    24361 agccagtgcg ctctgatagc atgtgcaaat tctttagcat ccatatcctt tgatgaaata
    24421 taatatcgga tgcttacacc ttctgctgat ttatcttctt tcttctgcct gaatgacaat
    24481 gctacacaaa gcttttttaa tcccttccat tcgaattcaa aatcacaaaa ttcaggcgtt
    24541 acgttactga caatatgcaa acgtgtttct tttcttccat gacttatctc ctgcgtacta
    24601 aacgaatcgc ctttataatt agaaaacaca tttacaggaa atttttcctc gaatgcatga
    24661 tgtaatttcc cctgattgcc ttttactgcc agaagataat ctgctttttt atctttgatc
    24721 ttcgaagcga tatctttctg acagcccata gcatcaatgg ttatcaaatt tttctttaaa
    24781 tccagtaggt taagcaactc tggaatggct gtaatctcat tacttttggc ttccgttttt
    24841 acctgcccca gtacaacacc attttcgttc gagaatgcac tcaccatatg gattgctcct
    24901 tttctttttc ccttatcaaa ggagcctctt atggtctttc catctattgc tataatttca
    24961 ccatcagtga tttcatggca ctcctgcatc cattcaataa atatcttttc aaaggccaaa
    25021 ctgtcaatgt tactcacaac gcgtgcaatg gtgtcatcga ccggaatgcc attatcaaaa
    25081 tcaccatatt tctttagcca ttcaagtctt tcatgtccaa aatcttcaat ttcctgccac
    25141 tcatcggcac ctgcaattac tgcacatacg gtgagaaaca aaatatcaga taatttatgt
    25201 ttaacctttc cttgttgtcg tatatcaggg atcactgaaa tataatcaag caaactttga
    25261 atactcacct ggcgtccttt ttatcttttt agttattcca aaggttaaat tttgatcacc
    25321 tgtaggcgtt aaagccagga tgaaaatcgt gatttacatc acgattttca tgatcttgcc
    25381 ctgccccgtc ccttgctctc atgtctttct gtatgtattt tatatttttc tctttctggt
    25441 gttgttcatt atgaccattt accaatccag tcaaaaactt catttttcaa attgttatca
    25501 taataatcaa catcataata gtctccataa ttaattattt tataactagc cttcttgttt
    25561 ttatcactaa aatacacccg ttgttttgta gcgcagaact ccatattctc cagtttacta
    25621 atgtttttta atcgcacgct ataaagaata tcattgaaaa aattaagatt tatatcaacc
    25681 actccacagt gcgtcttgta attattaacc attatact
//
LOCUS       DAZFAB010000063        25639 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZFAB010000063 DAZFAB010000000
VERSION     DAZFAB010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25639
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.063"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(32..1483)
                     /gene="uxaB"
                     /locus_tag="ACHJWA_002930"
     CDS             complement(32..1483)
                     /gene="uxaB"
                     /locus_tag="ACHJWA_002930"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFO4309483.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(1710..3128)
                     /locus_tag="ACHJWA_002931"
     CDS             complement(1710..3128)
                     /locus_tag="ACHJWA_002931"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO4309484.1"
                     /translation="MHVHPISTFRVFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLAINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPQMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(3267..3626)
                     /gene="yneG"
                     /locus_tag="ACHJWA_002932"
     CDS             complement(3267..3626)
                     /gene="yneG"
                     /locus_tag="ACHJWA_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFO4309485.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPHGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(3626..4552)
                     /gene="glsB"
                     /locus_tag="ACHJWA_002933"
     CDS             complement(3626..4552)
                     /gene="glsB"
                     /locus_tag="ACHJWA_002933"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFO4309486.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(4616..6004)
                     /gene="sad"
                     /locus_tag="ACHJWA_002934"
     CDS             complement(4616..6004)
                     /gene="sad"
                     /locus_tag="ACHJWA_002934"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFO4309487.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGADDIENALQLAAAGFRD
                     WRETNIDYRAEKLRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVTQMIKDSRIAAITVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMDDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITIAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            6105..6986
                     /gene="yneJ"
                     /locus_tag="ACHJWA_002935"
     CDS             6105..6986
                     /gene="yneJ"
                     /locus_tag="ACHJWA_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO4309488.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAIVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNRRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWCWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSTKQGYQ"
     gene            7064..8179
                     /gene="yneK"
                     /locus_tag="ACHJWA_002936"
                     /pseudo
     CDS             7064..8179
                     /gene="yneK"
                     /locus_tag="ACHJWA_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            8330..9520
                     /gene="ydeA"
                     /locus_tag="ACHJWA_002937"
     CDS             8330..9520
                     /gene="ydeA"
                     /locus_tag="ACHJWA_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFO4309489.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGTVPAFAALIWSIIIFRRWPVTLE
                     EQMQ"
     gene            complement(9545..10210)
                     /gene="marC"
                     /locus_tag="ACHJWA_002938"
     CDS             complement(9545..10210)
                     /gene="marC"
                     /locus_tag="ACHJWA_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFO4309490.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNGAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TNH"
     gene            10422..10856
                     /gene="marR"
                     /locus_tag="ACHJWA_002939"
     CDS             10422..10856
                     /gene="marR"
                     /locus_tag="ACHJWA_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFO4309491.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            10876..11259
                     /gene="marA"
                     /locus_tag="ACHJWA_002940"
     CDS             10876..11259
                     /gene="marA"
                     /locus_tag="ACHJWA_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFO4309492.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            11291..11509
                     /gene="marB"
                     /locus_tag="ACHJWA_002941"
     CDS             11291..11509
                     /gene="marB"
                     /locus_tag="ACHJWA_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFO4309493.1"
                     /translation="MKPLLSAIAAALILFSAQGVAEQTQQPLVTSCGDVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQQAM"
     gene            complement(11540..12439)
                     /gene="eamA"
                     /locus_tag="ACHJWA_002942"
     CDS             complement(11540..12439)
                     /gene="eamA"
                     /locus_tag="ACHJWA_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFO4309494.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAISGVLVLIEDSFNGQHVAMLGFMLTLAAAFSWASGNI
                     FNKKIMSHSSRPAVMSLVVWSALIPIIPFFVASLILDGSGKMIHSLVTIDMTTILALM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMAGLYINVFGLQWRKAVKVRG"
     gene            12634..13821
                     /gene="ydeE"
                     /locus_tag="ACHJWA_002943"
     CDS             12634..13821
                     /gene="ydeE"
                     /locus_tag="ACHJWA_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HFO4309495.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTVGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMFFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLIGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAVNPLVSGVVLTTLPSSSLFIILALVIIAAWALMLKGINARPQRQP
                     ALC"
     gene            complement(13862..14257)
                     /locus_tag="ACHJWA_002944"
     CDS             complement(13862..14257)
                     /locus_tag="ACHJWA_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFO4309496.1"
                     /translation="MRYVTEFTTAKPEVAEAHFLARLSVETDCADVHDSLAYIGDDFV
                     LLHVVGREEDFARRHIPGALHLPHKQMTAERMAEWPDDTLFVVYCAGPHCNGADRAAL
                     KLARLGKPVKIMLGGITGWEDEGYAFASE"
     gene            14352..15323
                     /gene="ftrA"
                     /locus_tag="ACHJWA_002945"
     CDS             14352..15323
                     /gene="ftrA"
                     /locus_tag="ACHJWA_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FtrA"
                     /protein_id="HFO4309497.1"
                     /translation="MTTLQRPLVIALAYDGLCTFEFGVAVEIFGLPRPELGDNWYRFA
                     VAAVEPGALQATGGIHVVADGGLELLPNADMIIIPGWRGIDAKVPEMLCQSLREAHQR
                     GTRLVSICSGVFVLAAAGLLHQRKATTHWRYTEQLQHRYPSISVSDNVLYQDEGQVMT
                     SAGSAAGIDLCLHIVRKDFGHEIANNIARRLVIQPHRQGDQPQNLTRPMASPRESQTL
                     GALFDFLQQNLAQTHTVSSLAERVNMSPRTFLRRFEALTGMTPARWLLNERLIRVQDY
                     LQNSVFNIEKIAELTGFGNSALLRHHFHQRFLLSPSQYRKNSRSPSQ"
     gene            15431..15526
                     /gene="mgtS"
                     /locus_tag="ACHJWA_002946"
     CDS             15431..15526
                     /gene="mgtS"
                     /locus_tag="ACHJWA_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFO4309498.1"
                     /translation="MLGNMNVFMAILGIILFSGFLAAYFSHKWDD"
     gene            15526..15630
                     /gene="mgtT"
                     /locus_tag="ACHJWA_002947"
     CDS             15526..15630
                     /gene="mgtT"
                     /locus_tag="ACHJWA_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFO4309499.1"
                     /translation="MNGDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(15745..16635)
                     /gene="dgcZ"
                     /locus_tag="ACHJWA_002948"
     CDS             complement(15745..16635)
                     /gene="dgcZ"
                     /locus_tag="ACHJWA_002948"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFO4309500.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLRPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGVLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEETLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(16890..17282)
                     /gene="ydeI"
                     /locus_tag="ACHJWA_002949"
     CDS             complement(16890..17282)
                     /gene="ydeI"
                     /locus_tag="ACHJWA_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFO4309501.1"
                     /translation="MKFQAIVLASFLVIPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKTMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPATVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
     gene            17558..18076
                     /gene="ydeJ"
                     /locus_tag="ACHJWA_002950"
     CDS             17558..18076
                     /gene="ydeJ"
                     /locus_tag="ACHJWA_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFO4309502.1"
                     /translation="MNINRNKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            complement(18121..20166)
                     /gene="dcp"
                     /locus_tag="ACHJWA_002951"
     CDS             complement(18121..20166)
                     /gene="dcp"
                     /locus_tag="ACHJWA_002951"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFO4309503.1"
                     /translation="MTTINPFLVQSTLPYLAPHFDQIANHHYRPAFDEGIQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQATLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGLRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            20303..21049
                     /gene="ydfG"
                     /locus_tag="ACHJWA_002952"
     CDS             20303..21049
                     /gene="ydfG"
                     /locus_tag="ACHJWA_002952"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFO4309504.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASIEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            21138..21824
                     /gene="rspR"
                     /locus_tag="ACHJWA_002953"
                     /gene_synonym="ydfH"
     CDS             21138..21824
                     /gene="rspR"
                     /locus_tag="ACHJWA_002953"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFO4309505.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            22001..22204
                     /gene="ydfZ"
                     /locus_tag="ACHJWA_002954"
     CDS             22001..22204
                     /gene="ydfZ"
                     /locus_tag="ACHJWA_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFO4309506.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(22240..23700)
                     /gene="ydfI"
                     /locus_tag="ACHJWA_002955"
     CDS             complement(22240..23700)
                     /gene="ydfI"
                     /locus_tag="ACHJWA_002955"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFO4309507.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLHQQDNLYTVAEMSADAWTARVVGVVKKALHVQI
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYALMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GGDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(23789..25072)
                     /gene="ydfJ"
                     /locus_tag="ACHJWA_002956"
                     /pseudo
     CDS             complement(23789..25072)
                     /gene="ydfJ"
                     /locus_tag="ACHJWA_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
     gene            25204..25446
                     /locus_tag="ACHJWA_002957"
     CDS             25204..25446
                     /locus_tag="ACHJWA_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO4309508.1"
                     /translation="MPHRYPAAKINKMPKGSVPALQQEMLRRVSKRYDDVEVIIKSTS
                     NDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVR"
     gene            complement(25443..25577)
                     /locus_tag="ACHJWA_002958"
     CDS             complement(25443..25577)
                     /locus_tag="ACHJWA_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HFO4309509.1"
                     /translation="MKLILGTACKTRHLVCKNVLRLAGELSIVRVLNDFQPLLILLVN
                     "
BASE COUNT         6735 a         6502 c         5856 g         6546 t
ORIGIN      
        1 aagaaacccg gtcgaaaccg ggttcagagt attagcacaa cggacgtaca gcttcgcgca
       61 tccctttttc gagaatcgca tccaggtcat tagccacctg ctcgaccaga ccaggcactt
      121 gcgtcaggtc ctgctcccag tggtcttttt ctgccaacac aatcgctacc agttcctggg
      181 taccgataac acggtcacga tgctgactcc agagttgctg ataacgttcc agccagtgtg
      241 catcgtcctg taccggatat gtttctccat tacgctcacc gcgatagaac gcaatcaatg
      301 ccgctaatgc gaaagtaagg cgcgccggaa gtgtgccgtt tgccttctgc cctgccagca
      361 gctgcggcag gatgcgggta cggaacttgg tcataccgtt gagcgcgata gacagcagct
      421 gatgcttaat gtacgggtta cggaaacgcc cggtgactgc actggcgaaa gattccagtt
      481 catcgcgtgg caaatccagt accggaataa tttcttcgta gatggctttt tcaacgaatg
      541 cgcaaatttc agcatcgttc atcgcttcac ctacggtatc cagccctgcc tggaacgcca
      601 ccggcaccaa cgcggtgtgt gcaccgttga gtattgccac tttgcgttct ttatacggtt
      661 taatatcgtc aacaatcagc acgttgagcg gatatttgtc cagacgcagc tcggtcgcta
      721 aggatttcgg tccctgaatc acaaacaagt aaaagtgttc agcggtgtcg aggaaaccat
      781 cgtgataacc cagttcttct tccagtttag ccacttcatc acgcggataa ccggtaacga
      841 tacggtccac cagcgtagaa cagaagctgt tagcctgatc cagccattga ataaatgctt
      901 ctggcagtgc ccactcttgc gcatagcgca gcaccagttc acgcaacgcg tcgccattgt
      961 agtcgatcaa ctcacacggg atgatgatcc aacctttatc cagcgcaccg ttgaaatggc
     1021 taaaacgttc gaacagcaga cgggtcagtt ttgccggata acttaccgct ggcgcgtcat
     1081 cgaatttatc gcccgcatgg tagctgatac ccgcttcggt ggtgttagag aaaacaaagc
     1141 gcatttccgg gttgtgcgcc agtttcagga attcatcgta ttcactgtag acgctgattt
     1201 cacgattaac agagcgaatc agacgcgcgt cgctgaccgc ttcccccttc tcattcaggc
     1261 cacggataat ggtggtgtac agaccatcct gcgtgctcag tgacggcggg aatgaagttt
     1321 caatcggacg aacaacgacc acaccagaat tcagatcggt gtgctcattc aggagatcta
     1381 tttgccagtc aacaaaggcg cgcaggaagt taccttcacc aaactgaatg atacgttctg
     1441 gatactgtgc accgggaaaa tcgcgacgat ttagtgtttt cacaatgggt tcccttctga
     1501 ttagtcatac aacctgtttg aattggtacg acaggttagc aaactttaat acgacgaacc
     1561 cctgttttga tcaactcctg atgattaatg agcagtttta tgagaaaagt gtggcgcgga
     1621 tcatggttta atcgaggaaa tacgcctttt cctggatcat aaagtggtag aacacattgc
     1681 attcaaatcg cgcggaatga ataaagatgt cagacaactt cctcaccgta acgcatagtg
     1741 ctggtacggt tgcgcccatc tttcttcgag cgatacagac aggtatcggc ttcgaccatc
     1801 aatttattaa agtcatcggt cagcgttcgg tacgatgcgc gaccactacc gacgccaata
     1861 cttaccgtga gataaagcgt tttttgttgc caggtgaatg gttgcagttc aacgccttta
     1921 cggatttttt ccgccattag cagaccatcg acaggattca ctgacggcac tgcaacagca
     1981 aattcttcgc cgcccatgcg cgccaccagc cccttatcac cgacaatctt ctgaatatgc
     2041 cgggcaaaca cgcttaacac tttatcgcca cattcatgtc catagttatc gttaatactt
     2101 ttgaaatagt cgatatcaag tagcatgaca gtcagatgtt gtgtctgttt cagcgatgga
     2161 cttttcagcg cctcataaag accggaccgt gagtaaacct gagtcagaaa atcaaagtcg
     2221 gctcgcagcg caacttgctt cattagcgaa ttgatcgctg ccacgctaaa agaaaccata
     2281 attgggcata tcgccatcgt ggcaataccg agacgtgcgg agaacatttg cggaatggag
     2341 aacggcgaac cgaccgagat atcaatcacc gaattagcca ccagtacgat ttccaccgca
     2401 ccggtgacaa aggtcaacag acatgttacc tgcggcgtat agcgcactgc acaccagatt
     2461 aatgcaggca gcgggaacgc cagactcccc gctccgccaa tgactaccga ggcaataacc
     2521 gacacaataa gcgcgatggc gggcatcatc tgttctgctt taaagcgcgg cagcactcca
     2581 ggaatagcca acgtcagcat acaaggcacg atcaacacgc ccgttgagaa ttgctcactg
     2641 aaccagtccg caagcaaagg ccagaaatcc agactatcaa tactgaccga accaatcgcc
     2701 ccgacaatag cgcataataa tgcggcaatc agacagtaat taaatagccg taaggcactt
     2761 actggctcat atttattttt ccccaaacgc ttatcccgca cgaccagtaa ggcgacggta
     2821 acaataaaca tcatattgga gaaattgata gccagtgaaa ccagtcccca ttcggtggtt
     2881 atggcatcat aaaccagcat cgccacataa ctgatcgcat agtaatgcag tcgattaagc
     2941 cagacatagc gggcaaacac ccccgccatt acgccattca gcggccagaa aagagaaagt
     3001 tcgtgaacca gccgtaactc tgcaccaata aaatagaaca gcgtggttag cgcaaaaata
     3061 gcgatgctat tacgtagcag atgaccttcc tggaacacac ggaaagttga aatggggtgt
     3121 acatgcatta attatctgtc catacaattg cgggtggcaa ttatctcaac gtaataagta
     3181 aattctcata aaggatagca tgttcgctga cagcggcggc aaataaaggt accctgggaa
     3241 tgctattttg tcgttttccg caattctcat ggctgattca tctgtacaac cagccagtga
     3301 tagattaccg ccacgatata acgttgttgt tcttcgctta gcgaaacacc atgcgggatg
     3361 ttatgccact tagccagaca accgcgacag caggtcgcgg tggcgtgctg agcgataaac
     3421 accggatgcc cgcgcatggg ggtttgctta ccgtcattag ccggtaatgc cggagcaaga
     3481 cgtttagcga caaaatccgc cgcatgttgt tcgatgactg gcgcgccttt ctccagacaa
     3541 tactgacgct ctttcatgcc caaacgaaag cgagatcgaa attttgaacg ggataaacgc
     3601 gcgaagagtg gatcgagaga ctgcattaat aaaccgaacg ccctaactgt ttcgtcaatt
     3661 gttcaaggac ggcgataccc gcaagcgagt tacctgcatc atccagttcc ggactccaga
     3721 ctgcgatggc catttcatgc ggaacaatcg ccacaatacc gccaccaacg ccagatttcg
     3781 ccggtagtcc cacccgccag gcaaactccc ccgcgttctg gtacatacca ctggtcgcca
     3841 tcagcgcgtt aatttgccgt gcctgcattg gcgtcaccac aggttcatca atatgaatag
     3901 ctttcccctg attagccaga aagacaaacg tccgggccag ctctacacag ctcattttca
     3961 gagcgcagta atgaaaatag ttttgcagaa cggttgtcac gtcatgatgg aaattgccaa
     4021 acgacttcat cagccaggcg atagccgcat ttcgcgcgga atgttcaaat tcggaacgcg
     4081 ctaccaccgt atcgtaggaa atatcagaca caccgcttaa gccgcgcacg acttccagca
     4141 tacgttgccg tggtgcgctt aatcgccctt gcaacatatc gcagaccacc agcgcaccag
     4201 cattaataaa cggattacgc ggaataccct gctccatttc cagttgcact aaagaattga
     4261 acggtgatcc agacggatct ttgccgacgc gttgccagat ttcctcttcg gagtaatgac
     4321 gcatggcgac aacgagactc agcactttgg aaatagactg aatggaaaaa cgttcttgcg
     4381 cgtctccggc ctgaaaaagc tgtccgtcaa cggtacagat agcaatgccc aatcgggaac
     4441 cgtctactgt agccagcgcc ggaatataat ccgcgacttt accctgacca atgagcggcc
     4501 gcacttgccg caagatgttt tctaaaattg cattatccat ggcgactgcc actttctact
     4561 cctggaccgc aggtctgaaa agacctgcga gtatatcaga gctgaatatg tcgcgtcaga
     4621 tccggtcttt ccacaccgtc tggatattac agaattcgtg taagccgaaa tgggaaagct
     4681 cacgaccaaa gccactcttt ttcacgccac caaaggccac tcgcgcgtcg ctggcacaat
     4741 aaccattgat aaacacccca ccgcattcca gacgtgccgc catctgtctg gcctgtgttt
     4801 catcggtggt aaaaatggtc gctgaaaggc cgaactcact atcattagcc agttccagcg
     4861 catgttctgc atctttcgca atggtgattg ccgcaacggg gccaaacatt tcttcccgaa
     4921 aagcggtcat ttctggggta acattcgcca gaaccgttgg cggatagtag ttacctgccc
     4981 cagccatctt ttccccgccc agtaacaaac gcgcaccctg cgccagggtt ttctccacct
     5041 gatgatgcag ctcatcacgt aaatcgaaac gagccattgg tccgagagcg ttctcttcgt
     5101 cacggggatc gtccattttc aaggctgccg cagctgccac aaaacgttcg gtaaatgccg
     5161 aagcaattcc ctcttcgata ataaagcgtt ttgccgctgc acaaacctgt ccggtattct
     5221 gataacgtcc ggctaccgcc gctttaactg ccagttccag atcggcatcg ttaagcacaa
     5281 taaatggatc cgaaccgccc agttccagta cgcatttttt cagtgccgct ccagcctgtg
     5341 caccaatagc cgctccagca cgaacacttc cggtcaccgt gatagcagca atgcgcgagt
     5401 ctttaatcat ctgagtgaca ccgtcattgt cggcattcag ccagccatat acgccttgtg
     5461 ggattcccgc atctttaaac acctgggcaa tgagctgagc acagcccatc acattcggcg
     5521 catgtttaag taagtatccg ttaccagcaa gaatgatggg aacagcgcca cgcatcacct
     5581 gccataacgg aaaattccac ggcataatcg ccagaatcgt ccccaacggt cgatactcaa
     5641 taactgcctg ctgattttcc accagcgtag gttccgcctt cagcattgcc ggaccatgtt
     5701 ctgcatacca gtcacacaaa ttcgccgatt tcgccacttc agcgcgcgcc tggttgattg
     5761 gtttgcccat ttcgcgggtg atcatttgcg ccatttcttc gctacgggcg cgcagagcct
     5821 taccgatatc acgcagtttt tcagcacgat aatctatatt tgtctcgcgc cagtcacgaa
     5881 agcctgctgc cgccagctga agtgcgtttt cgatatcatc agcgccagcc cacggcaaca
     5941 cagaaagttg ttcacccgtg gcaggattta tcgaaattgc atgagttgcc ggagtaatgg
     6001 tcatcggggt atctccttta tgagtcatgg tatgaagata cgcagattta ctcttgcttt
     6061 aaaatgaata atattaagcc acttattcac gaatcgagaa tattatggat ctgacccaac
     6121 tggagatgtt caacgcggtt gccgaagctg gcagcattac gcaggctgca gcaatagtgc
     6181 atcgcgtgcc gtcgaatctc actacccgtc tacgccagct ggaaacagaa ctgggggtag
     6241 atctgtttat tcgcgagaat cagcgtttac gtctatctcc tgccgggcat aactttttac
     6301 gctatagcca acaaattctc gcgttagtgg atgaagcgcg gagcgttgtc gctggcgatg
     6361 agccgcaagg tttattttct ctgggttcgc tggaaagcac cgcagcagtt cgcattccgg
     6421 ccacgctggc ggaattcaac cgtcgttatc ccaaaattca gttttcactt tccaccggcc
     6481 cttccggcac tatgctggaa ggtgtgctgg agggaaaact taatgcggcg tttattgatg
     6541 gacccattaa ccatactgcc attgacggga taccggtata ccgcgaggag ctgatgatcg
     6601 tcacgccaca aggtcatgcg ccagtaatcc gtgccagtca ggttaatggc agtaacattt
     6661 atgccttccg cgccaattgt tcgtatcgtc gccacttcga gagctggttt catgctgacg
     6721 gtgccgctcc gggaactatc catgagatgg agtcttatca cggaatgttg gcctgtgtgg
     6781 tcgcaggagc aggtattgcg cttattccgc gctctatgct ggaaagtatg ccggggcatc
     6841 accaggttga agcgtggccg ttagctgagc aatggtgttg gctaacaacc tggctggtct
     6901 ggcgtcgtgg tgcgaaaacg cgtccgctcg aggcatttat tcaactactg gatgtgcctg
     6961 actcgacaaa acagggttac caatgagcta tttttgatag ttcttgcggc taatatgctc
     7021 tatatagtga tgttccgatg acttatgact atatggggca aatatggtta cgccagtaag
     7081 catcagcaat tacatatctc ttcctgatga ttttcccgtt cgtaatattg caccgcaggt
     7141 aaaagaagtt ttaaaggatt ttattgatgc acttagtaca ataatatgta atgaagagtg
     7201 gcgcacgtct ttaaacatca actcagccac aaaaaagata tttaataatc ttgacaactt
     7261 atcatatatt cagaggactt cttttcgggg taacgacacg ctatacaatg aaaaggttca
     7321 gtttaaactc acttatcccg ccaggaatgg aagacacaaa gaaaatattg aatttcaggt
     7381 agtaataaat ttaagcccta tttatttaga taatttccgc catgatggag aaattaatat
     7441 tttttgcgct cccaacccaa agcctgttac tatggggcgc gtatttcaga ccggcgtcga
     7501 gcgtgtgctc tttctgtttc tgaatgattt tattgaacaa ttcccaatga tcaaccctgg
     7561 cgttcccatc aaaagagcgc atacaccaca tattgaaccc ctgcctccgg atcaccatac
     7621 cgctgcagat tacttacgcc agtttgattt gcttgtcctg aattttatct ctcgcggtaa
     7681 ttttgtcata ctcccccgat tatggaataa ctctgaggtt cacagatggt tcgtcaataa
     7741 agatcctaac ttgatcaccg ccattctcga cataacggac agcgaattaa aagaggattt
     7801 attgcaaagc ctgatggatt cattaggttc taacaaacat gtactaccct aagtctgcat
     7861 ctgtttttta tcccttttag cagaacaaga atctcctcat tttcaggact tgtttttatt
     7921 tttcgccaat atgttgttac actatcacca atttatgaat cccaatgaaa gtgatttgaa
     7981 tgacgtgttg atgccagcat cattaagtga tgataaaatt atcaaacata tggcgcgtaa
     8041 gaccctcaaa ctgtttgtaa aaaatgaaac accgccaaaa gttactcatg aagatctggt
     8101 gaaaaacagg cctcgctccc ctgtcagacc gcctataccc gcaacctcca aaacgccaga
     8161 cattcctgat cgtcattaaa ccgtgatgtt taccatctct ctgacgcgtg aaagaatcag
     8221 cgtcagagaa acggaaaacg cgatccagat cacaaatgca ttgtattcac atcattaacc
     8281 gttttaagat catttcatca ctttttcgca actcacccga taatctgtta tgacaacaaa
     8341 cactgtttcc cgcaaagtgg cgtggctacg ggtcgttacg ctggcagtcg ccgccttcat
     8401 cttcaacacc accgaatttg tccctgttgg cctgctctct gacattgcgc aaagttttca
     8461 catgcaaacc gctcaggtcg gcatcatgtt gaccatttac gcatgggtag tagcgctaat
     8521 gtcattgcct tttatgttaa tgaccagcca agttgaacgg cgcaaattac tgatctgcct
     8581 gtttgtggtg tttattgcca gccacgtact gtcgttttta tcgtggagct ttaccgttct
     8641 ggtgatcagt cgcattggtg tggcttttgc acatgcgatt ttctggtcga ttacggcgtc
     8701 cctggcgatc cgtatggctc cggccgggaa gcgagcacag gcattgagtt taattgccac
     8761 aggtacagca ctggcaatgg tattaggatt acctctcggg cgcattgtgg ggcagtattt
     8821 cggttggcga atgaccttct tcgcgattgg tatcggggcg cttattaccc ttttgtgcct
     8881 gattaagtta cttcccttac tgcccagtga gcattccggt tcattgaaaa gcctcccgct
     8941 attattccgc cgcccggcat tgatgagcat ttatttgtta actgtggtgg ttgtcaccgc
     9001 ccattacacg gcatacagct atattgagcc ttttgtgcaa aacattgcgg gattcagcgc
     9061 caactttgcc acggcattac tgttattact cggtggtgcg ggcattattg gcagcgtgat
     9121 tttcggtaaa ctgggtaatc aatatgcgtc tgcgttggta agtacggcga ttgcgctgtt
     9181 gctggtgtgc ctggcactgc tgctacctgc ggcgaacagt gaaatacacc tcggggtgct
     9241 gagtattttc tgggggatcg cgatgatgat catcgggctt ggtatgcagg ttaaagtgct
     9301 ggcgctggca ccagatgcca ccgacgtcgc gatggcgcta ttctccggga tatttaatat
     9361 tggaatcggg gcgggggcgt tggtaggtaa tcaggtgagt ctgcactggt caatgtcgat
     9421 gattggttat gtgggcacgg tgcctgcttt tgccgcgtta atatggtcaa tcattatatt
     9481 tcgccgctgg ccagtgacac tcgaagaaca gatgcaatag ttgaaaggcc cattcgggcc
     9541 ttttttaatg attcgtttta atgatttcca ggatgccgtt aataataaac tgtacaccca
     9601 tacataccag caggaacccc atcaagcggg agatcgcttc aatgccaccc ttgcccacca
     9661 gccgcataat tgcgccagag ctgcgtagac ttccccacaa aataaccgcc accaggaaaa
     9721 agatcagcgg cggcgcaacc atcagtaccc aatcagcgaa ggttgaactc tgacgcactg
     9781 tggacgccga gctaataatc atcgcaatgg ttcccggacc ggcggtactt ggcattgcca
     9841 gtggtacaaa agcaatattg gcgctgggtt catcttccag ctcttccgac ttgcttttcg
     9901 cctccggtga atcaatcgct ttctgttgcg gaaaaagcat ccgaaaaccg ataaacgcga
     9961 cgattaagcc gcctgcaatt cgcagaccgg gaatcgaaat gccaaatgta tccatcacca
    10021 gttgcccggc gtaatacgcc accatcatga tggcaaagac gtacaccgag gccatcaacg
    10081 actgacgatt acgttcggca ccgttcatgt tgcctgccag gccaagaaat aacgcgacag
    10141 ttgttaatgg gttagctaac ggcagtaaca ccaccagccc caggccaatt gctttaaaca
    10201 aatctaacat tggtggttgt tatcctgtgt atctgggtta tcagcgaaaa gtataagggg
    10261 taaacaagga taaagtgtca ctctttagct aaccttgcat cgcattgacc aaaacttgaa
    10321 ccgatttagc aaaacgtggc atcggtcaat tcattcattt gacttatact tgcctgggca
    10381 atattatccc ctgcaactaa ttacttgcca gggcaactaa tgtgaaaagt accagcgatc
    10441 tgttcaatga aattattcca ttgggtcgct taatccatat ggttaatcag aagaaagatc
    10501 gtctgcttaa cgagtatctg tctccgctgg atattaccgc ggcacagttt aaggtgctct
    10561 gctctatccg ctgcgcggcg tgtattactc cggttgaact gaaaaaagtg ttgtcggtcg
    10621 acctgggagc actgacccgt atgctggatc gcctggtctg taaaggctgg gtagaaaggt
    10681 tgccgaaccc gaatgataag cgcggcgtac tggtaaaact taccaccagc ggcgcggcaa
    10741 tatgtgaaca atgccatcaa ttagttggcc aggacctgca tcaagaatta acaaaaaacc
    10801 tgacggcgga cgaagtggca acacttgagc atttgcttaa gaaagtcctg ccgtaaacaa
    10861 aaaagaggta tgacgatgtc cagacgcaat actgacgcta ttaccattca tagcattttg
    10921 gactggatcg aggacaacct ggaatcacca ctgtcactgg agaaagtgtc agagcgttcg
    10981 ggttactcta aatggcacct gcaacggatg tttaaaaaag aaaccggtca ttcattaggc
    11041 caatacatcc gcagccgtaa gatgacggaa atcgcgcaaa agctgaagga aagtaacgag
    11101 ccgatactct atctggcaga acgatatggc ttcgagtcgc aacaaactct gacccgaacc
    11161 ttcaaaaatt actttgatgt tccgccgcat aaataccgga tgaccaatat gcagggcgaa
    11221 tcgcgctttt tacatccatt aaaccattac aacagctagt tgaaaacgtg acaacgtcac
    11281 tgaggcaatt atgaaaccgc ttttatccgc aatagcagct gcgcttattc tcttttccgc
    11341 gcagggcgtt gcggaacaaa cccagcaacc gctcgttact tcctgtggcg atgtggtggt
    11401 tgttccccca tcgcaggaac aaccaccgtt cgatttaaat cacatgggta caggcagtga
    11461 caaatcggat gcgctgggcg tgccctatta taaccaacaa gccatgtagt atattcaggc
    11521 cccggcatat cggggcttgt taacctctta ccttcaccgc tttacgccac tgcaaaccaa
    11581 atacattgat atacagtccg gccataatga gcacagcccc taagaattgc agacctgtta
    11641 agcgttcatc cagcagtaac gctgcacttg ccagaccgac taccggcacc agtaatgata
    11701 atggtgcgac acgccatgtt tcatagcgtc caagcagcgt accccaaatc ccgtagccaa
    11761 ctatcgtcgc tacaaatgcc agatacatca acgccagaat ggtggtcatg tcgatagtga
    11821 ccagactgtg aatcattttt ccggagccat cgagaatgag cgaggcgaca aaaaacggaa
    11881 ttatcgggat taatgcgctc cagacgacca gagacattac cgccggacgg gatgaatgag
    11941 acatgatctt tttgttgaag atattgccac tcgcccaact aaatgccgct gccagagtca
    12001 acataaagcc gagcatcgcc acatgctgac cgttaaagct atcttcgatt aacaccagta
    12061 cgccagaaat cgctaacgca atccccgcca gttgcttacc gtgcagccgt tcgccaaaag
    12121 taaacgcgcc aagcacgata gtaaaaaacg cctgagcctg taagaccagc gaggccagtc
    12181 cagccggcat accgaagtta atggcgcaga agagaaaggc aaactgtgca aaactgatgg
    12241 ttaaaccata ccccagcagt aaattcagcg gtacttttgg tcgtgccacg aaaaagattg
    12301 ccgggaaggc gaccagcata aagcgcaaac cggccagcat cagcggtggc atgttatgaa
    12361 gccccacttt gatgaccaca aaattaagcc cccatacaac gacgactagt agcgccaaca
    12421 ccccatcttt tcgcgacatt ctaccgcctc tgaatttcat cttttgtaag caatcaactt
    12481 agctgaattt acttttcttt aacagttgat tcgttagtcg ccggttacaa cggcattaat
    12541 gcgcaaataa gtcgctatac ttcggatttt tgccatgcta tttctttaca tctctaaaac
    12601 aaaacataac gaaacgcact gccggacaga caaatgaact tatccctacg acgctctacc
    12661 agcgcccttc ttgcctcgtc gttgttatta accatcggac gcggcgccac gctgccattt
    12721 atgaccattt acttgagtcg ccagtacagc ctgagtgtcg atctaatcgg ttatgcgatg
    12781 acaattgcgc tcactattgg cgtcgttttt agcctcggtt ttggtatcct ggcggataag
    12841 ttcgacaaga aacgctatat gttactggca attaccgcct tcgccagcgg ttttattgcc
    12901 attcctttag tgaataacgt gacgctggtg gtgctctttt ttgccctcat taactgcgcc
    12961 tattccgttt ttgctaccgt gctgaaagcc tggtttgccg acaatctttc atccaccagc
    13021 aaaacgaaga tcttctcgat caactacacc atgctcaata ttggctggac cgtcggcccg
    13081 ccactcggca cgctgttggt catgcagagc atcaatctgc ccttttggct ggccgctatc
    13141 tgctctgcgt ttcccatgtt tttcattcaa atttgggtaa agcgcagcga gaaaatcatc
    13201 gccacggaaa caggcagtgt ctggtcgccg aaagttttat tacaagataa ggcactgttg
    13261 tggtttacct gctctggctt tctggcttct tttgttagtg gcgcatttgc ttcatgcatt
    13321 tcacaatatg tgatggtgat tgctgatggt gattttgccg aaaaggtggt cgcggttgtt
    13381 cttccagtga atgctgccat ggtggttacg ttgcaatatt ccgtgggccg ccgccttaac
    13441 ccggctaaca tccgcgcgct gatgacagca ggcaccctct gtttcgtcat cggtctgatc
    13501 ggttttattt tttccggcaa cagcctgcta ttgtggggta tgtcagctgc ggtatttact
    13561 gtcggtgaaa taatttatgc gccgggcgag tatatgttga ttgaccatat tgcgccgcca
    13621 ggaatgaaag ccagctattt ttccgcccag tcattaggct ggttgggcgc agcggtaaat
    13681 ccattagtga gtggtgttgt acttaccact ctcccgtcgt cttcgctgtt tatcattctg
    13741 gcgttagtca tcattgccgc gtgggcgctg atgttaaaag gtattaacgc cagaccccag
    13801 aggcaaccag cactctgttg acgttcgtgc cgttccacta ctggcacggc ttcgctaatc
    13861 attattctga ggcaaaggca tagccttcat cttcccagcc ggttatcccg ccgagcatga
    13921 ttttaaccgg ctttccgagt cgagccagtt tgagtgccgc acggtctgca ccgttacaat
    13981 gtggccccgc gcaatacacc acaaatagcg tatcgtcggg ccattcggcc attctttctg
    14041 cggtcatttg cttatggggt aaatggagag cgcctgggat atgccgacgc gcgaagtcct
    14101 cttcacgtcc aaccacatga agtaaaacga aatcatcgcc aatataagcc agagagtcat
    14161 gaacatcggc gcaatctgtt tcgacactca agcgcgcgag aaaatgcgcc tcagcaactt
    14221 ctggcttcgc tgtggtaaat tcagtgacat aacgcatatt gctttgtcct tttggtttgt
    14281 tgtgttaaca ataatgtaac taatctacct ttcactgacc gcctctcgat atgccagaaa
    14341 tgagcaaaat gatgacaacg ttacaacgtc cgctggtcat tgcactggca tatgacgggc
    14401 tatgtacttt tgaatttggc gttgccgttg aaatattcgg cctgccacga ccggaactgg
    14461 gcgataactg gtatcgtttt gctgttgctg cggtagaacc aggtgcgcta caagccaccg
    14521 gggggatcca cgtggttgct gatggtggac tggagttatt gcccaacgcc gatatgatta
    14581 ttattcctgg ctggcgcgga atagatgcca aagtgccgga gatgctatgc cagtcattgc
    14641 gcgaggcaca ccagcgcggt actcgcctgg tatcaatatg ctctggcgtg tttgtccttg
    14701 ctgctgcggg ccttcttcat cagcgaaaag caaccaccca ctggcgctac actgaacaac
    14761 tccagcaccg ttacccttca atcagcgtaa gtgacaatgt gttatatcag gatgaaggac
    14821 aagtgatgac ctccgccgga agcgccgcgg ggatcgatct gtgtttacat atcgtgcgca
    14881 aagattttgg tcatgagatt gctaataata tcgcccggcg actggtcatt cagccacatc
    14941 gccaggggga tcaaccacaa aatctgacgc gcccaatggc ctcaccgcgt gaaagccaga
    15001 cgttaggtgc cttattcgac tttttgcagc agaatctggc acaaacacat actgtgtctt
    15061 ccctcgccga acgggtaaat atgagtccgc gcactttttt aagacgtttt gaggcgttaa
    15121 ctggcatgac gcccgcccgc tggctgctca atgagcgctt aatacgagta caggattatc
    15181 tgcaaaattc ggtttttaac atcgagaaaa ttgccgagct gacgggtttt ggcaattcag
    15241 cgttattgcg tcatcacttt caccaacgtt ttttattatc tccttcgcaa tatcgcaaaa
    15301 acagccgttc cccatcacaa taatcgcctg attcagcctt tgtttagata acctctgcta
    15361 atatctttct cattgagata aaaattaagg taagcgagga aacacaccac accataaacg
    15421 gaggcaaata atgctgggta atatgaatgt ttttatggcc atactgggaa taattttatt
    15481 ttctggtttt ctggccgcgt atttcagcca caaatgggat gactaatgaa cggagataat
    15541 ccctcaccta accggcccct tgttacagtt gtgtacaagg ggcctgattt ttatgacggc
    15601 gaaaaaaaac cgccagtaaa ccggcggtga atgcttgcat ggatagattt gtgttttgct
    15661 tttacgctaa caggcatttt cctgcactga taacggatcg ttgacacagt agcatcagtt
    15721 ttctcaatga atgttaaacg gagcttaaac tcggttaatc acattttgtt cgtcaataaa
    15781 catgcagcga tttcttccgg tttgcttacc ctcatacatt gcccggtctg ctcttccaat
    15841 gaccacatcc agagtctctt caggaaatgc gcgactcaca cctgctgtca cggtaatgtt
    15901 gatatgccct tcagaatgtg tgatggcatg gttatcgact aactggcaaa ttctgacacc
    15961 tgcacgacat gcttcttcat cattagccgc tttgacaata atgataaatt cttcgccccc
    16021 gtagcgataa accgtttcgt aatcacgcgt ccaactggct aagtaagttg ccagggtgcg
    16081 taatactaca tcgccgatta aatgcccgta ggtatcatta accaatttaa atcggtcaat
    16141 atccaacaac attaaataaa gattcagagg ctcagtgttg cgtaactgat gatcaaagga
    16201 ttcatcaaga acccgacgcc ccggcaatcc cgtcaaaaca tccatattgc tacggatcgt
    16261 cagcaaataa attttgtaat cggttaatgc cgcagtaaaa gaaagcaccc cctcctgaaa
    16321 ggcgtcgaaa tgcgcgtcct gccagtgatt ttcaacaata gccagcatta attcccgacc
    16381 acaattatgc atatgttgat gggcagaatc cattagccga acgtaaggta attcatcgtt
    16441 atcgagtggc ctcagatgat caatccaccg accaaactgg cacagtccat aagaatggtt
    16501 atccgttatt tctggcttac tggcatctct cgcgaccacg ctgtgaaaca tactcaccag
    16561 ccactgatag tgggcatcga tagccttatt gagatttaac aagatggcat caatttccgt
    16621 tgtcttcttg atcattgcca ctcctttttc acagttcctt gtgcgcgcta ttctaacgag
    16681 agaaaagcaa aattacgtca atattttcat agaaatccga agttatgatt catctctgag
    16741 ataacattgt gatttaaaac aaaatcagcg gataaaaaag tgtttaattc tgtaaattac
    16801 ctcttcatta tcgcaaatga aaggatgaca aatagcataa cccagtaccc aaatggcccg
    16861 gtagttcagg ccatcaggct aatttatttt tatttctgca aatgagtgac ccgaacgacg
    16921 gccggcgcgc ttttcttatc cagactgccg ctaatgttga tcatctggtc cggctgaact
    16981 tctcgtccat caaaaacggt cgcaggaata acaacattaa tttcaccact cttatcgcga
    17041 aaaacgtaac ggtcctctcc tttgtgagaa atcaaattac cgcgtagtga aaccgaagcg
    17101 ccatcgtgca tggtttttgc gaaatcaaca gtcatttttt ttgcatcatc ggttccgcga
    17161 tagccatctt ctattgcatg aggcggcggt ggcgctgcat cctgttttaa accgccctgg
    17221 tcatctgcca acgcataagg tatgacaaga aaacttgcta atacaatggc ctgaaatttc
    17281 atactaactc cttaattgcg tttggtttga cttattaagt ctggttgcta tttttataat
    17341 tgccaaataa gaatactgcc aattgttata aggcatttaa aatcagccaa ctagctgtca
    17401 aatattcaga gaatttaact cactaaagtt aagaagattg aagagtctta aacatatttt
    17461 cagaataatc ggatttatat gtttgaaaat tattatattg gacgagcata cagaaaaagc
    17521 aaatcacctt tacatataaa agcgtggaca aaaaacagtg aacattaata gaaataaaat
    17581 tgtacaactt gcagataccg acactattga aaacctgaca tccgcgttga gtcaaagact
    17641 tatcgcggat caattacgct taactaccgc cgaatcatgc accggcggta agttggctag
    17701 cgccctgtgt gcagctgaag atacacccaa attttacggt gcaggctttg ttactttcac
    17761 cgatcaggca aagatgaaaa tcctcagcgt aagccagcaa tctcttgaac gatattctgc
    17821 ggtgagtgag aaagtggcag cagaaatggc aaccggtgcc atagagcgtg cggatgctga
    17881 tgtcagtatt gccattaccg gctacggcgg accggagggc ggtgaagatg gtacgccagc
    17941 gggtaccgtc tggttcgcgt ggcatattaa aggccagaac tacactgcgg ttatgcattt
    18001 tgctggcgac tgcgaaacgg tattagcttt agcggtgagg tttgccctcg cccagctgct
    18061 gcagttactg ctataaccag gctggcctgg cgatatctcc aggacagcca ttggtggtgt
    18121 ttatatgttc aagccacgat gttgcagcat cggcataatc tgaggagcct taccgcgcca
    18181 ttgtcgatac aggcgttcca gatcttcgct gttacctctg gaaaggatcg cctcgcgaaa
    18241 acgcagccca ttttcacgcg ttaatccgcc ctgctcaaca aaccactggt aaccatcatc
    18301 ggccaacatt tgcgtccaca gataagcgta ataacctgcg gcatatccgc caccaaaaat
    18361 atgggcaaaa taactgctgc gatagcgtgg cggtatagca ggaagatcca tattttctgc
    18421 caccagcgcc cgcaattcaa aatcatcgac atcctgcatt gcttcgtttt cttccaggca
    18481 atgccagcgc atatcgagaa gtgcggcgct aagcagttcg ctcatctcat accctttgtt
    18541 gaacaggctg gcattacgca ttttctgttg cagttcgtca ggcattgctg ccccgctctg
    18601 ataatgccgg gcgtagcgag cgaatacctg cggatgcgtt gcccagtgtt cgttgatttg
    18661 cgacggaaat tcgacaaaat cacgcggcgt gttggtgccg gagagcgtgg cataacgctg
    18721 gcgggcaaaa aggccgtgca gcgtatgacc aaattcatgg aataaggtta tgacatcatc
    18781 ccagagtaac aacgcaggct caccggcagc gggtttctga taattgcaga cgttataaat
    18841 taccggatgc gtttcattaa gcgttgattg ctcaacaaaa ttgcccatcc atgcaccgcc
    18901 gctttttgaa tcacgggcga agaaatcacc gtaaaataac gccagcccca cgccattatg
    18961 atcaaaaatt tcccacaccc gaacgtcagg atggtagaca ggaatatcaa aacgttcgac
    19021 aaacttaata ccgaagagct gattcgcggt ccagaataca ccttcattca acaccgtgtt
    19081 taattcaaaa tatggcttga gctgcgcctc atcaagatca aatttctctc gccggacctg
    19141 ttcggcataa aatgcccagt cccacggctg cgcgctaaac ccgccctgct gcttatcaat
    19201 aaccgcctgt atggaggcta attcatcgct cgcacgttga cgcgccgctg gaacaatttc
    19261 ccgcataaag ttgagtgctg cttcaggtgt ttttgccatc tgatcggcga ttttccatgc
    19321 ggcataatga ggaaaaccga gtaatgtagc ctgttgtgca cggatctcca ccagacgttg
    19381 aatgatagcg cgggtatcat tggcatcatt tttttccgct cgcgtccagc ccgcaataaa
    19441 cagtttttca cgcgtcgcac gatcgcgcat ttcggcaagc gccggttgtt gggtggtatt
    19501 cagcagcgga atcagccatt tgttatccag acctttctcg cgagccgcct ctgccgccag
    19561 cgcaatctct tgctcactca ttcctgccag ctgcgcgata tcgttcacaa ccagaccgcc
    19621 ggatttattt gctgccagta accgctggtt aaactgactg gtcagtgtcg cagcttctgt
    19681 attcagtact tttaattttg ctttatcagc ttgtgcaagt ttggctccgg ctaggacaaa
    19741 acgttgatga atcacctcca ccaggcggat ggattcacta tcaagcccca gagattcacg
    19801 gcgttgccag acagcatcta cccgcgcgaa taattcaccg ttcagataga tatcattagc
    19861 cagttccgcc agttcagcgg aaaactgctc gtcaagacgc tgtaattcat cattggtatg
    19921 cgccgcagtc atcgcaaaaa agacgctggt aacgcgggta agtaattctc cgctttgttc
    19981 cagtgccaga atagtattgt tgaaatcagg tgtttgcggg ttaagcgcga tggcagcaat
    20041 ttctgcccgc ttttgctgta ttccctcatc gaatgccggg cgatagtgat gattggcaat
    20101 ttgatcaaaa tgaggagcca gatacggcag tgtgctttgc acaaggaaag gattaattgt
    20161 tgtcattttc ttctcctgaa cgcgaggtgt tccatagcgt aggcttactg ataacggagt
    20221 gcaatcttgc aaaccagtat tacccgctct taagcatcac gtgctatgtt attgacacac
    20281 aaaagcgttg aggaacagtg agatgatcgt tttagtaact ggagcaacgg caggttttgg
    20341 tgaatgcatt actcgtcgtt ttattcaaca agggcataaa gttatcgcca ctggccgtcg
    20401 ccaggaacgg ttgcaggagt taaaagacga actgggagat aatctgtaca tcgcccaact
    20461 ggacgttcgc aaccgcgccg ctattgaaga gatgctggca tcgcttcctg ccgagtggtg
    20521 caatattgat atcctggtaa ataatgccgg tctggcgttg ggcatggagc ctgcgcataa
    20581 agccagcatt gaagactggg aaacgatgat tgataccaac aacaaaggcc tggtatatat
    20641 gacgcgcgcc gtcttaccgg gtatggttga acgtaatcat ggtcatatta ttaacattgg
    20701 ctcaacggca ggtagctggc cgtatgccgg tggtaacgtt tacggtgcga cgaaagcgtt
    20761 tgttcgtcag tttagcctga atctgcgtac ggacctgcat ggtacggcgg tgcgcgtcac
    20821 cgacatcgaa ccgggtctgg tgggtggcac cgagttttcc aatgtccgct ttaaaggcga
    20881 tgacggtaaa gcggaaaaaa cctatcaaaa taccgttgca ttaacgccag aagatgtcag
    20941 cgaagccgtc tggtgggtgt caacgctgcc tgctcacgtc aatatcaata ccctggaaat
    21001 gatgccggtt acccaaagct atgccgggct gaatgtccac cgtcagtaat ttttataccc
    21061 ggtgtaactg ccgggttatt gcttgtcaca aaaaagtggt agactcatgc agttaactca
    21121 ctcacaagca agaacgaatg accgtcgaaa cgcaacttaa tcccacacag cctgtcaatc
    21181 agcagattta tcgtattctt cgtcgcgaca ttgtccattg cctgattgca ccaggcacac
    21241 cgttgtcgga aaaagaagtt tctgttcgtt tcaatgtgtc acgccagccg gttcgtgaag
    21301 cctttattaa actggcggaa aacggcctga ttcaaattcg tccgcagcgt ggcagctacg
    21361 tcaacaaaat ttccatggcc caggtgcgca acggcagttt tatccgtcag gccattgagt
    21421 gcgcggtggc gcgtcgggcg gcgagtatga ttaccgaaag ccagtgctac caactggaac
    21481 aaaatcttca ccagcaacgc attgccattg agcgcaagca actggatgat ttttttgaac
    21541 ttgatgacaa cttccatcaa ctcctgacgc agattgccga ctgtcaactg gcgtgggata
    21601 ccattgagaa cctgaaagcg accgttgatc gcgtgcgcta tatgagtttc gaccatgttt
    21661 ctccaccaga aatgctgtta cgccagcatc ttgatatatt ctctgccctg caaaaacgtg
    21721 atggcgatgc ggtagaacgt gcaatgacgc aacatttgca ggaaatcagc gaatccgtgc
    21781 gccagatccg ccaggaaaat agcgactggt ttagcgaaga gtaattcatt tcctctcatc
    21841 ccatccgggg tgagagtctt ttcccccgcc ttatggctca tgcatgcatc aaaaaagatg
    21901 tgagcttgat caaaaacaaa aaatatttca ctcgacagga gtatttatat tgcgcccgtt
    21961 acgtgggctt cgactgtaaa tcagaaagga gaaaacacct atgacgacct acgatcgtaa
    22021 ccgtaacgca atcaccactg gcagccgtgt tatggttagc ggcaccggtc acactggcaa
    22081 gatcctgtcg attgatactg aaggtctgac cgctgagcaa atccgccgcg gaaaaaccgt
    22141 agttgttgaa ggttgtgaag agaaactggc accactggac ctgattcgtc tcggcatgaa
    22201 ctaagcgtgt gaatgccgcc gatggcggca ttgctttttt tacttcacgg aatatttcgc
    22261 cacggtcgct ttcgcaccat gcgctaataa agacaagtac gcttccgtca cttttgcagt
    22321 aaacaagcta ttgtctggca aatcaccacc aaaaatcgcc ttaatcgcca gcaatgactg
    22381 gacgcgcgct ttcccttcgg cactactttg tacagccttc tgaataacag gtaacagtgg
    22441 gtcactgatt tctatcggat ttccctgttc atcaacacca ccgacataac gcatccaacc
    22501 cgcgacgccc agcgccagca gatcgaactt gctgtcatgc gccagatgcc agcgaacaga
    22561 atccaacatc cgctgtggca atttctggct gccatccatc gcaatctgcc aggttcgatg
    22621 acgtaacgcc gggttgctat agcgtgcaat taatcggtta gcgtaatctt gcaaatcaac
    22681 gccctgcact ttcagcgtcg gcgcttgttc ctgcaacatc aaggcatacg ccgcatgacg
    22741 ataatgttca tcttccatac agtcattaat gtgctgatat ccggcaagat aacccagata
    22801 cgccaggaat gaatgactgc cgttgagcat gcgcaacttc atctcttcat aaggcagcac
    22861 atcactaacc agttcggctc ccgctttttc ccattccgga cgtccggcaa caaagttatc
    22921 ttcgattacc cactggcgga aaggttcaca ggcaacgcca gcaggatcgc gcacaccggt
    22981 aagctgttcg attttcgcca gcgtatcctc tgtcactgcg ggcacaatac ggtccaccat
    23041 tgttgatggg aaagtcacgt tatcttcgat ccattgtgct agttttacat caacggcttg
    23101 tgcgtaggaa gtgacaacgt cacgcataac atgaccgttt tctggcatgt tgtcacatga
    23161 catgacggta aatgcgggaa gtcctgccgc tttacggcga gccagcgcct caacaatcac
    23221 ccctgttgct gttttcggct ggtggggatt ttgcacgtcg gcagcgacca tcgggtgatc
    23281 gagcattaac tgtccggtcg ccggagagtg gaaataccct ttttcggtga ttgtcagaga
    23341 gacaatcgcg atttgcggtt cacacatcgc agccaacacg gtttctaagc catctatctg
    23401 tacgtgcaag gcttttttaa cgacgccaac gacgcgagcc gtccacgcat cggccgacat
    23461 ttccgcaacg gtataaagat tatcttgctg atgtaaatcg gcaatttgct gttcgccgcc
    23521 gattaagttg acctcataat atccccagtc actgaaatgt tctgtagcaa gaatatcggc
    23581 atacacgccc tgatgcgcac ggtgaaatgc accaaagcct aaatgaacaa ttcttggggc
    23641 caggttatta cgatcataaa cagggagtgt cgcttttgct gataacaaat tatttcccat
    23701 aacaattcct taaatttaaa tatggcaagc tacatgtttt gttatatgaa taaaaatccc
    23761 ctctccggta agagaaggga ttgaggattt acagacttct ggaaggttgc gcagctctta
    23821 caacacgcgg ttgatcttcc gcagcgtctt ccagcgcact taaatcacgg tctttcactt
    23881 ctggcatttt cagcgcagag attaaaccaa tcactgaata tgccatgatc ataatggcga
    23941 tcggatacca ggattccgtc atggtgcaga aaatacccgc caggatagga ccaaaaccgg
    24001 aagcgataag accaccaatt tctttagaaa tagccatccg ggtaaagcgg tttttacagc
    24061 cgaacatttc tgccatggta atgttttcca gagcaaataa tcccagcacc gcacagttat
    24121 gaatcacaat cagtgcaacc ataatggtgc tcggggcata gcttttatct acgatgatag
    24181 aaagcattgg ccatgccagc acaatcgcgg aggtattcat aataatatac gggatccggc
    24241 gaccaatttt atcggataac caaccaagga acggaatggt cataaagccg ggaatcgaac
    24301 tgatcatcaa tgcatctgtt ggaattgctt tgttaaacaa taacgtctgt actaaatagc
    24361 ctgcaaggaa agtctgaatt aacccggagt tacctgcctg accaaaacgc agccctgttg
    24421 ccagccagaa ggatttgctc tggaatatgc taccagcagg tgcaggtttt gctgtcggtt
    24481 ggttgctgtc gttaaccttc tcaaagaccg ggctttcttt cagattcata cgcaaccaga
    24541 tagcaaagac catcacgaca acgctcgcca ggaacggtat acgccatccc cacgccagca
    24601 gttcctcttt actgagaatg aagaacataa aggcccagat tgccgttgcg ctcaaggttc
    24661 cgcagttagt tcccatagcc acaaatgagg agataattcc gcgcttacct tttggcgcat
    24721 attccgccag catcgtaccg gcaccggaaa tttccgcacc tgcacccaat ccctgaataa
    24781 tacgcagcgt caccagcaag atgggggcaa aaacaccaat ctgtgcatag gtcggtaaca
    24841 ctccaattaa ggtggtacag atccccatca tggtgatggt aataaagagc acttttttac
    24901 gccctattct gtcgcccatt ttgccgaaaa taaatgctcc gacaatacgc gccacataac
    24961 ctgcaccgta ggttcccatt gccagaatta acgccattgc cgttgatgat tcaggaaaaa
    25021 atatttcatg aaacactaac gctgcgccga gcgaatataa ctggaaatcc ataattcata
    25081 gatgtttttt actattctgt gggtttttgg gtgttttcta agttttttca gatggttgta
    25141 ttttttctaa aaatccctaa tctcgatttt tctgtttatt tgaggccttt ttatgtccca
    25201 tatatgcccc acagataccc cgcagccaaa atcaacaaaa tgccaaaagg ttctgttcct
    25261 gccctgcaac aagaaatgct gcgacgtgtc agtaaacgtt atgacgatgt agaagtgatc
    25321 atcaaatcca ccagcaacga tggcctttca gttactcgca ccgccgataa agattctgca
    25381 aaaacttttg ttcaggagac gctgaaagat acctgggagt ctgctgacga gtggtttgtt
    25441 cgctaattaa cgagtaaaat cagtaacggc tggaaatcat tcaatactcg cactatcgaa
    25501 agttcaccag ccaaccgcag cacgttcttg catacgaggt gccgcgtttt acatgctgta
    25561 ccgagaatta atttcatttt acgaaaagtt aacccgccat tgctatctta attttttaaa
    25621 acgaagttac ctctgtcag
//
LOCUS       DAZFAB010000064        25501 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZFAB010000064 DAZFAB010000000
VERSION     DAZFAB010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.064"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..117)
                     /locus_tag="ACHJWA_002959"
     CDS             complement(<1..117)
                     /locus_tag="ACHJWA_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4309510.1"
                     /translation="MTISNSNLLKPFITGTELPPFKEDVFSKTEEQFKKSTYG"
     gene            complement(305..2122)
                     /gene="malZ"
                     /locus_tag="ACHJWA_002960"
     CDS             complement(305..2122)
                     /gene="malZ"
                     /locus_tag="ACHJWA_002960"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFO4309511.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNLQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGREVARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     MALFALYQRMIALRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPFLNVVQWQRKEGHGQLTDGILALPAISATVWMN"
     gene            complement(2278..3651)
                     /gene="proY"
                     /locus_tag="ACHJWA_002961"
     CDS             complement(2278..3651)
                     /gene="proY"
                     /locus_tag="ACHJWA_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFO4309512.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(3727..5046)
                     /gene="brnQ"
                     /locus_tag="ACHJWA_002962"
     CDS             complement(3727..5046)
                     /gene="brnQ"
                     /locus_tag="ACHJWA_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFO4309513.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(5453..6748)
                     /gene="phoR"
                     /locus_tag="ACHJWA_002963"
     CDS             complement(5453..6748)
                     /gene="phoR"
                     /locus_tag="ACHJWA_002963"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFO4309514.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSVISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIVKNSD"
     gene            complement(6806..7495)
                     /gene="phoB"
                     /locus_tag="ACHJWA_002964"
     CDS             complement(6806..7495)
                     /gene="phoB"
                     /locus_tag="ACHJWA_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFO4309515.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            7685..8887
                     /gene="sbcD"
                     /locus_tag="ACHJWA_002965"
     CDS             7685..8887
                     /gene="sbcD"
                     /locus_tag="ACHJWA_002965"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFO4309516.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            8884..12027
                     /gene="sbcC"
                     /locus_tag="ACHJWA_002966"
     CDS             8884..12027
                     /gene="sbcC"
                     /locus_tag="ACHJWA_002966"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFO4309517.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIEEVNTRLQNTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLMLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLLQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            12153..13337
                     /gene="araJ"
                     /locus_tag="ACHJWA_002967"
     CDS             12153..13337
                     /gene="araJ"
                     /locus_tag="ACHJWA_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFO4309518.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(13480..14388)
                     /gene="mak"
                     /locus_tag="ACHJWA_002968"
     CDS             complement(13480..14388)
                     /gene="mak"
                     /locus_tag="ACHJWA_002968"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFO4309519.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            14513..15424
                     /gene="rdgC"
                     /locus_tag="ACHJWA_002969"
     CDS             14513..15424
                     /gene="rdgC"
                     /locus_tag="ACHJWA_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFO4309520.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMANPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(15509..15958)
                     /locus_tag="ACHJWA_002970"
     CDS             complement(15509..15958)
                     /locus_tag="ACHJWA_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4309521.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNGGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKNYCPP"
     gene            complement(15955..18234)
                     /locus_tag="ACHJWA_002971"
     CDS             complement(15955..18234)
                     /locus_tag="ACHJWA_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
                     /protein_id="HFO4309522.1"
                     /translation="MNRINILVICMVLFFMTGNACATEWISSEDLITSDFHLMTADER
                     NVVKAATDDSMEAAYMLKDNIRWYYHNGDLSLPANFSNKNKLVVNGNLTISGDYDDYL
                     SGNGHLIVLGNVIVDNFINHDFAYVKGQMTAKGLVYADYNDHNFEVMKGISARGIIVS
                     DKATQFEVIKAEFYINEDGSGEGYNWDENIQKAYSLVTADLYDHTEIETDNISNAYPD
                     YDSVADNIVQGLPLFRDKAAPEINEKLKWIETGKLDNFPANKIKHQDPLVARFLTHTE
                     SLSPAVMLQLLQHPDDQTRESMAQSWPAQQMHWLTDELIKDEAVARGLVKNSNISADV
                     NKKLMSVPVESVQLEQARQDNLSPDIVASLSHSPFLSVRKTLLSHYDYAWLVPTAVAD
                     ELINNEDPELRERITGADLTAQQAVMLSKDKSLKVREALARTLTELKITQLSATLRTE
                     DIERIAEQMYLDNKENKNIVKVLLIALPEMRQLSLAKEDVHNLREGARYLTSKDVISY
                     LLTQHDVPTVWDELARDKLLPLEYKKQLWQRTLNLMMSKRQEDQEQAYEVQLALIDNG
                     VVDEEMLNNAIDLLVDLPAEYRYRMRNQLFDNKELPSGIINKLDQQYRFNSDWALAVV
                     SMKNSTRRQSERGLHRWNHEDSDIFAELATIKDKSDDEWWRALLQSRNDHLRQTALRN
                     AHTPASLLTTLTESQDRSLAINNPQLAADVKTVWLKEDPSLLLFVDKPDLSQLRDLVK
                     TGATRKIRNEARHRLEEKQ"
     gene            complement(18378..18662)
                     /gene="ppnP"
                     /locus_tag="ACHJWA_002972"
     CDS             complement(18378..18662)
                     /gene="ppnP"
                     /locus_tag="ACHJWA_002972"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFO4309523.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(18734..19411)
                     /gene="aroM"
                     /locus_tag="ACHJWA_002973"
     CDS             complement(18734..19411)
                     /gene="aroM"
                     /locus_tag="ACHJWA_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFO4309524.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNHLAHVSRRKVERDLQGVVEVLDNQGYDVIILMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEELLTVQAQKWQILQKPPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFNQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(19669..19860)
                     /gene="yaiA"
                     /locus_tag="ACHJWA_002974"
     CDS             complement(19669..19860)
                     /gene="yaiA"
                     /locus_tag="ACHJWA_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFO4309525.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(19910..20434)
                     /gene="aroL"
                     /locus_tag="ACHJWA_002975"
     CDS             complement(19910..20434)
                     /gene="aroL"
                     /locus_tag="ACHJWA_002975"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFO4309526.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(20617..21075)
                     /gene="yaiI"
                     /locus_tag="ACHJWA_002976"
     CDS             complement(20617..21075)
                     /gene="yaiI"
                     /locus_tag="ACHJWA_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFO4309527.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            21195..22004
                     /gene="proC"
                     /locus_tag="ACHJWA_002977"
     CDS             21195..22004
                     /gene="proC"
                     /locus_tag="ACHJWA_002977"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308464.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFO4309528.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDKFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(22021..23136)
                     /gene="adrA"
                     /locus_tag="ACHJWA_002978"
                     /gene_synonym="dgcC"
     CDS             complement(22021..23136)
                     /gene="adrA"
                     /locus_tag="ACHJWA_002978"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFO4309529.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGIWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVIYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVNLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(23238..23558)
                     /gene="psiF"
                     /locus_tag="ACHJWA_002979"
     CDS             complement(23238..23558)
                     /gene="psiF"
                     /locus_tag="ACHJWA_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFO4309530.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(23677..25092)
                     /gene="phoA"
                     /locus_tag="ACHJWA_002980"
     CDS             complement(23677..25092)
                     /gene="phoA"
                     /locus_tag="ACHJWA_002980"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFO4309531.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTADQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(25193..25453)
                     /gene="iraP"
                     /locus_tag="ACHJWA_002981"
     CDS             complement(25193..25453)
                     /gene="iraP"
                     /locus_tag="ACHJWA_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFO4309532.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT         6422 a         6763 c         6221 g         6095 t
ORIGIN      
        1 gccataggtg ctttttttaa attgttcttc tgttttagaa aagacatcct ctttaaaagg
       61 tggcaactca gtaccagtaa taaacggttt caataaattc gaatttgaaa ttgtcatatc
      121 tttgctccac tacagggtta tcctggagca tgttgtacat aacatcatgg gcaaacaata
      181 ccaaaaatcg ctcatatcga tcgggacaaa tatttaacaa attcagccct gacattgagc
      241 agcttttttg cgctgcgtag tgtcagggct ggtgactaac gacgtaacaa ataaaacaga
      301 tgctttagtt catccacacc gtagccgaaa tggcaggcaa agccagaatc ccgtctgtca
      361 gttgcccatg gccttctttg cgttgccatt gcacgacatt aagaaacggt gacgcgggta
      421 gcaccacttc acaggcttcg ccacggttaa ttgccaccag tacacgctgc tgattcagca
      481 cgcggacaaa taccaccacg ttatcttccg catacagcac ctgacagccg ccacgacgca
      541 gcgcctgact tttcttacgc agcgcaatca ttcgctggta caacgcgaat aacgccatat
      601 cctgtttttc cacctgccag gggaacggct tacggcaaaa cggatcattt ttgccatcca
      661 gtcccacttc atcaccgtaa taaatgcacg gtacaccagg ccaggtgaac agccagacca
      721 ctgccagggg caggcgcgca acctcccgac cgagcagcgt tttaaatcgc gcagtatcgt
      781 ggctgtcgag ctgattaaac atacgtaatt gctgttgatg agaaagccct gcgcggtaat
      841 tatccatcca ggccatacag gtttgagcgt caatttgctg cggatcgtaa gagatgtcgg
      901 tattggcaag aaatccccac aacgggaatg tgaagccgcg atagttcatg gcggcatctt
      961 ccacatcggc ctgtaaccat tgccgtgcat cgccaaaatg ctctccaact atatacgctt
     1021 ccggctgggt ttctttcgcc gcttcggtga tcccagcaac gtgctgcaaa ttattgcgcg
     1081 ccccacccgc ttcccccagc atatgcacca catccagccg ccagccgtcc atattccacg
     1141 gcgctttcag ccagtggcgg acaatactgt cttccccgcg ataaatttca ttcaccagac
     1201 tttccgactg ataatccagc ttcggcaagc tggcatagcc aagccagtcg agcgccgtgc
     1261 catcatcact aaacgagtac cagtcgcgcc agggcgattc ggggttgtga caagctcccc
     1321 ccgtgccacg attgtgcctg tcaaaccagg catgggaatc gccactgtgg ttaaacacgc
     1381 catccagcac cagccgcatt cccagctgct gcgtattgtg gcgtaagcgc agcaacgccc
     1441 catcaccgcc aaactgcgga tcgacatggc gataatcctc ggtatcgtat ttatgtacgc
     1501 tgggagcttt aaacaccgga ttgagataca gcgccgtcac gccaagcttt ttcagatacg
     1561 gcagtttttc gcttatcccg tccagatcgc cgccatagaa cgttgatccg cccgcctgcg
     1621 ccgtgaccgg ttcatcccag tcacgcaaga tgatctcttg tccggctgca tgatggtaat
     1681 agacatgatc ctgttcagct tcacgaggaa gactacgcgc aaaacgatca gggaagatct
     1741 gataaaaaat ctgatccgca gcccattgtg ggccgatatc cggtacatcg acggcaaact
     1801 gctccagtcg tgccggcggc attcggctga agccctgcgg tgtaaaccaa cgctggcgat
     1861 cgtgccacag cagtttgaaa ctgtaacgcc gccggggttg tccgctggag agatcaatcg
     1921 ccgcacgcca tgcggtgacg cctggctgcg gctgactgcg ctgcttatgc attggtactg
     1981 acatttcttc gttatcgtgt tctgtacgca gcataatgcg ctgcggtggg tcttcgcccg
     2041 tcagccacag tgtaatgagc agttgatctt tgctttgttt aacaaatggg ggcaccggca
     2101 ggtgccatgc atttaacatc ataattcccc tgtgatgaaa atctgatcac cttgccatag
     2161 cctaatgcag gatcactcat catctgaagg atatttgggg gaggaggacg ggggcggata
     2221 actttcggaa tagccggatc aggatttcct cctgaaccgg cttacagaac gcaagtatta
     2281 ttggttttca gccagctgac gatcgtggcg acgcttaaac atccagccaa tcaacagcac
     2341 aacaatccac gcgaaaccga catacagcga gatacgcgta tccgggtgat aaccaatcaa
     2401 cccgataata aagagcagga aaatcagccc gccgatggtc gttgctaccc cacccggcac
     2461 tttaaatttc agcgccttaa cttcttctgg cggcaaacgg cgacggaagg caatttgcga
     2521 cagcaggatc ataatccaca cccacaccgt ggcgaaggtt gccagcgaag cgatcaccag
     2581 gaagacgttt tccggcatga tgtagttcag atacaccgca aacagcagcg cggtagtcat
     2641 caccagcacc gtaacccacg gaataccgcg acgcgacgtt ttgctgaaaa ttttcggcgc
     2701 gctgccctgc tctgccatac cgtggagcat acggcctacg ccaaatacat cactgttaat
     2761 tgccgacagc gaagcagtca gcacaacaaa gttaagaatg ctggcggcaa aggtaatgcc
     2821 catatgctgg aacgtcagca cgaacgggct accggcagtg ccaacctgat tccacgggta
     2881 gatagacata atgacgaaca gcgtaccgac gtagaacacc agaatacgca tcggcacgga
     2941 gttaatcgca cgcggtatcg atttctcagg atctttcgct tcaccggcgg taatcccgat
     3001 aatttcgatc ccaccgtaag caaacatcac catttgcaac gacattacca tgccaagcca
     3061 gccgttactg aagaagccgc cgttgctcca caggttatga ataccggtcg gttgcccgcc
     3121 gttgccaatc ccccagatga tgatgccgaa accggcgaca atcatgatga tgatggtggc
     3181 gactttaaag aacgagaacc agaattccag ctcaccgaat accttcacgc tcatcaggtt
     3241 tacggcgcaa atgatcagca ccacgctcag tacccaaatc cagtgcggca ccgtcgggaa
     3301 ccagacaccc atatagatac caaaagcggt cacatcggcg atggcgacaa taaggatttc
     3361 aaagcagtag gtccagccgg taatgtaacc tgccagcggg ccgaggtttt cctgcgcata
     3421 acgcgagaaa gagctggcgg ccgggttatg taccgacatt tcccccagcg cacgcataat
     3481 gatatacgcc gcgataccac cgataatata ggccaacaac acgctcggac cggccatttt
     3541 gatggcgtct gccgaaccgt aaaacagccc ggtgccaatt gctgaaccca gtgccataaa
     3601 gcgtatgtgg cgggtactta gcccacgctt tagcttgttc ttactttcca tcaattccta
     3661 acccatcaac acaataaaaa accacggggc atcgcagccc cgtggttaaa acaaatgttc
     3721 agtgatttag tgagcgctgg aggtcacctg acgacctgcc gcgcgatccc agataatggc
     3781 cagaaccacc atcaccactg ttggcattaa ccacgccaga ccttgttcgg ccagcggtaa
     3841 acgctgcgcc caggacggta agatatcgct gaatgcagat gctttgatcc cgtcgagaat
     3901 accaaaaagc aggctgataa acatcggcgg agcaatcacg cgggacgaat tatgccacca
     3961 tgagcgtgta aaacttaata caaccagtgc gatacacggc ggataaatag cggtcagcac
     4021 cggtacggag atctggatca gctggcttaa gccgaggtta gaaaccacca tcgagaagcc
     4081 gccgaggata aacaccagcg tacgataaga gagcggtacg tactgggcaa agaattctgc
     4141 acaagcacag gtcaggccaa ctgccgttac caggcaggcg atgaagatta acgccgccag
     4201 caggaagcta ccgccgccgc caaaggtgtg ctgaacgtaa gcatgcagaa tagcagcgcc
     4261 gtttgcagac tgatcgacca gcgacgcgct gtctgaaccc agacggaaca gcgccaggta
     4321 cagcagagtc agaccaacac ccgccatcag gccagcccag acggtataac gggtcagtag
     4381 gcgcgcttcg gtaacgccac gagaacgcgc cgcgttaaca ataacgatac caaacaccat
     4441 tgcgcccagc gtatccatgg tcagatagcc gttgacgaag ccgttagaaa acgcagcgtt
     4501 ttgataagcc tcagtcgccg tgctgataga acccgccggc cagacaatcg cggcaacaga
     4561 caggatgacc agcgcgataa ttttcagcgg cgcaaggaag ttgcccacgg tatccagcag
     4621 cttgcccgga tagagcgaaa ccagaataac gatagcgaaa tagaccaggc tgtagataaa
     4681 cagcggcagc gcggaatcac ccgtcagcgg cgcaatcccc acttcaaaag aaacggtagc
     4741 tgtacgcggc gtagcgaaaa gcggccccac cgccaggtaa caaaccgttg ccagcagtac
     4801 gccagcgact ttaccgattg gcgtgctgag gctgtcaaca ccgccgccaa cttttgccag
     4861 cgccactacc gttaataccg gcaggccaac ggcagtaatg aggaagccga atgccgcagt
     4921 ccagacgtgt tcgcctgcct gtaagccgac cattggaggg aaaataatgt tacctgcgcc
     4981 gacgaacaac gcaaatgtca taaagcccag agcgatgata tcgcgcgatc ttaattgatg
     5041 ggtcataaat catactgcct gtggatgtgg tgttgtaacg ttaaattttt gtgccaatcc
     5101 ctggggatga tacagcggta tatttaaaca attttagcgg gaaatactcc caaccaggcg
     5161 cggcgaagaa tagtttttcg atttaccgcg ttaatgcagt cagtatgaca gcacctgaag
     5221 cgcaatttaa acgcttataa cgttttaaag caaggaggga tctaaaaacc agaggtatat
     5281 agcgataaga aatcaccaac cagtataaaa cactatttcc gtgaaaaaga catggctaat
     5341 catgcgaaca aaaaatcacc aaccgttgta aaatctaacg cccgtatttt gactgcaaag
     5401 aaaataagcc agcatcggct ggcttatggc aataaaagat gacaaaggcg gattaatcgc
     5461 tgtttttgac aattaaacgt tccgggataa caaaactgaa acgtgttcct tttcctactg
     5521 tactctcaat attcaggcga ctttcgtgat gattcaccgc atgtttcacg atcgctaacc
     5581 ctaatccgct accgccggtt tgccgggaac gcgctttatc aacgcgataa aaacgctcgg
     5641 tcaggcgcgg aatatgctcc ggtgcaatgc ccggtccgtt atcttcaacg ctaaattcgg
     5701 caccgtgcgg cactcgctgc cagcgtacgg tgatatgcgt gccttccggc gtatgattca
     5761 cggcgttata gaccaggttc gaaatcacac tgcgtagctg atcttcgttg ccagacacct
     5821 tgaggccgtt atctatctcg aaggtaaatg tctgtttttt ctgactcaga gtctgagcct
     5881 cgcgctcaac aacgcgcagc atcatcggca catcaacctt ttcattgagc aattgcgtcg
     5941 gtgcggcttc aattttcgac agcgttagca actgcttcac cagcccttcc atccgctggg
     6001 tctgctcgcg catggtgtgc aacgcttttt cgcgtaccgc gccttccagc ggctgctcat
     6061 ccatcatctc caggtaaccc tgtaacacgg tcaatggcgt acgtaactca tggctcacgt
     6121 tggcaaaaaa gttacgccgc gccccttcca gttgatgcat ttgcgtgaca tcacgcgcca
     6181 ccatcagcaa ctgtttgtgg gtataaggca tgacgcgaat ttccagatgc cgcccggtgt
     6241 tgagcaccag attgagcggg cgagaaaaat cacgcgtttt caaatattgc gtaaactccg
     6301 ggtaacgcag caggttaagg atgttctgcc cgttatcttc cggccagcgc aaaccaagaa
     6361 tctgttgcgc cagaccgtta caccagaaaa taccgccctc ttccgtggtc agcaccaccg
     6421 cgtcgggcag cgactccgcg ccgctacgaa agcgtttaat cagattgccc agttcacggc
     6481 ggcgtttttt atttcgcagc tgcatctggt gtaagccgta tagtagcggt tcccagctac
     6541 cacgccccgg tggcggggtc atactgcgat ccacccacag ccaccatgaa aggcgcaata
     6601 aattccagaa atgccagata agcagtcctg ttaccgatgc cagcaaaaac cagggcaggt
     6661 aaccaaaaaa tgcacccagg atgaaagccg ggaggcagca aagtagcagc tccagcacca
     6721 gccttttcca cgacagccgt tccagcacgt aagatactcc agttaagaaa tcataagccc
     6781 tgctctgcgt ccgatgagca aggcgttaaa agcgggttga aaaacgatat cctgtaccgc
     6841 gcacggtctg caccatgcgg tcatgcccgc cgggctccag tgctttacgc aggcgacgaa
     6901 tgtggacatc gaccgtgcgg tcttccacat acacgttagt tccccagacg tggtttaaca
     6961 gctgctcgcg gctgtacacg cgctcaggat gcgtcataaa aaagtgcagc agtttaaatt
     7021 ctgtcggccc catctccagc ggctcttcgc ccgccatcac tcggtgagat gtcgggtcga
     7081 gacttaatcc ctgcatctca atcacctctt ccaccgccat tggcgaaata cggcgcatta
     7141 ccgctttgat tcgcgccacc agctccttcg gcgaaaacgg cttggtgata tagtcatccg
     7201 cgccggtttc aaggccgcgc acgcgatctt cttcttcccc tctggcggtc aacatcacca
     7261 ctggaatatc ccgggtcatc gactcgcgct tgaggtgttt gatgaactgg ataccggagc
     7321 cgccaggtaa catccagtca aggagaatta aatccggcca gggttcattc agttgattca
     7381 cagcactgtc ataatcttcc gcttcgaccg gctgaaagcc attttgttcg agcacgaagc
     7441 agaccatttc gcgaattgga gcttcatctt ctacgaccag aatacgtctc gccatgattt
     7501 gccctgttgt aataaatagg ttgcgatcat taatgcgacg tcattatgcg tcagatttat
     7561 gacagattta tgaaaagctc gtcgcacata tcttcaggtt attgatttcc gtggcgcaga
     7621 aaaaagcaaa tggcacatct gtttgggtat aatcgcgccc atgctttttc gccagggaac
     7681 cgttatgcgc atccttcaca cctcagactg gcatctcggc cagaacttct acagtaaaag
     7741 ccgcgaagct gaacatcagg cttttcttga ctggctgctg gagacagcac aaacccatca
     7801 ggtggatgcg attattgttg ccggtgatgt tttcgatacc ggctcgccgc ccagttacgc
     7861 ccgcacgtta tacaaccgtt ttgttgtcaa tttacagcaa actggctgtc atctggtggt
     7921 actggcagga aaccatgact cggtcgccac gctgaatgaa tcgcgcgata tcatggcgtt
     7981 cctcaatact accgtggtcg ccagcgccgg acatgcgccg caaatcttgc ctcgtcgcga
     8041 cgggacgcca ggcgcagtgc tgtgccccat tccgttttta cgtccgcgtg acattattac
     8101 cagccaggcg gggcttaacg gtattgaaaa acagcagcat ttactggcag cgattaccga
     8161 ttattaccaa caacactatg ccgatgcctg caaactgcgc ggcgatcagc ctctgcccat
     8221 catcgccacg ggacatttaa cgaccgtggg ggccagtaaa agtgacgccg tgcgtgacat
     8281 ttatattggc acgctggacg cgtttccggc acaaaacttt ccaccagccg actacatcgc
     8341 gctcgggcat attcaccgcg cacagattat tggcggcatg gaacatgttc gctattgcgg
     8401 ctcccccatt ccactgagtt ttgatgaatg cggtaagagt aaatatgtcc atctggtgac
     8461 attttcaaac ggcaaattag agagcgtgga aaacctgaac gtaccggtaa cgcaacccat
     8521 ggcagtgctg aaaggcgatc tggcgtcgat taccgcacag ctggaacagt ggcgcgatgt
     8581 atcgcaggag ccacctgtct ggctggatat cgaaatcact actgatgagt atctgcatga
     8641 tattcagcgc aaaatccagg cattaaccga atcattgcct gttgaagtat tgctggtacg
     8701 tcggagtcgt gaacagcgcg agcgtgtgtt agccagccaa cagcgtgaaa ccctcagcga
     8761 actcagcgtc gaagaggtgt tcaatcgccg tctggcactg gaagaactgg atgaatcgca
     8821 gcagcaacgt ctgcagcatc ttttcaccac gacgttgcat accctcgccg gagaacacga
     8881 agcatgaaaa ttctcagcct gcgcctgaaa aacctgaact cattaaaagg cgaatggaag
     8941 attgatttca cccgcgagcc gttcgccagc aacgggctgt ttgccattac cggcccgacc
     9001 ggtgcgggga aaaccaccct gctggacgcc atttgtctgg cgctgtatca cgaaactccg
     9061 cgtctctcta acgtttcaca atcgcaaaat gatctcatga cccgcgatac cgctgaatgt
     9121 ctggcggagg tggagtttga agtgaaaggt gaagcgtatc gcgcgttctg gagccagaat
     9181 cgggcgcgta accaacccga cggtaatttg caggtgccac gcgtagagct ggcgcgctgc
     9241 gccgacggca aaattctcgc cgacaaagtg aaagataagc tggaactgac agcgacgtta
     9301 accgggctgg attacgggcg cttcacccgt tcgatgctgc tttcgcaggg gcaatttgct
     9361 gccttcctga atgccaaacc caaagaacgc gcggaattgc tcgaggagtt aaccggcact
     9421 gaaatctacg ggcaaatctc ggcgatggtt tttgagcagc acaaatcggc ccgcacagag
     9481 ctggagaagc tgcaagcgca ggccagcggc gtcgcgttgc tcacgccgga acaagtgcaa
     9541 tcgctgacag cgagtttgca ggtacttact gacgaagaaa aacagttact taccgcgcag
     9601 cagcaagaac aacaatcact aaactggtta acgcgtctgg acgaattgca gcaagaagcc
     9661 agccgccgtc agcaggcctt gcaacaggcg ttagccgaag aagaaaaagc gcaacctcaa
     9721 ctggcggcgc tcagtctggc acaaccggca cgaaatcttc gtccgcactg ggaacgcatc
     9781 gcagaacaca gcgcggcgct ggcgcatact cgccagcaga ttgaagaagt aaatactcgc
     9841 ttacagaaca caatggcgct tcgcgcgagc attcgccacc acgcggcgaa gcagtcagca
     9901 gaattacagc agcagcaaca aagcctgaat acctggttac aggaacacga ccgcttccgt
     9961 cagtggaaca acgaactggc gggttggcgt gcgcagttct cccaacaaac cagcgatcgc
    10021 gagcatctgc ggcaatggca gcaacagtta acccatgctg agcaaaaact taatgcgctt
    10081 gcggcgatca cgttgatgtt aaccgccgat gaagttgcta ccgccctggc gcaacatgct
    10141 gagcaacgcc cactgcgtca gcgcctggtc gcgctgcatg ggcagattgt tccccaacaa
    10201 aaacgtctgg cgcagttaat ggtcactatc cagaatgtca ctctggaaca gacgcaacgt
    10261 aatgccgcac ttaacgaaat gcgccagcgt tataaagaaa agacgcagca acttgccgat
    10321 gtgaaaacca tttgcgagca ggaagcgcgc atcaaaacgc tggaagctca acgtgcacag
    10381 ttacaggcgg gtcagccttg tccactttgt ggttccacca gccatccggc ggtcgaggcg
    10441 tatcaggcgc tggagcctgg cgttaatcag tctcgattac tggcgctgga aaacgaagtt
    10501 aaaaagctcg gcgaagaagg tgcggcgctg cgcgggcaac tggatgcatt aacgaagcag
    10561 cttcagcgcg atgaaaacga agcgcaaagc ctccgacaag atgagcaagc acttactcaa
    10621 caatggcaag ccgtcacggc cagcctcaat atcaccttgc agccacagga cgatattcaa
    10681 ccgtggctgg atgcacaaga tgagcacgaa cgccagctgc ggttactcag ccaacgacat
    10741 gaattacaag ggcagattgc cgcgcataat cagcaaatta tccagtatca acagcaaatt
    10801 gaacaacgcc agcagcagct cttaacggca ttggcgggtt atgcactgac attgccacag
    10861 gaagatgaag aagagagctg gttggcgaca cgtcagcaag aagcgcagag ctggcagcaa
    10921 cgccagaacg aattaaccgc gctgcaaaac cgtattcagc agctgacgcc gattctggaa
    10981 acgttgccgc aaagtgatga tctcccgcac agcgaagaaa ctgtggcgct ggataactgg
    11041 cggcaggttc atgaacaatg tctcgcatta cacagccagc agcagacgtt acagcaacag
    11101 gatgttctgg cggcgcaaag tctgcaaaaa gcccaggcgc agtttgacac cgcgctacag
    11161 gccagcgtct ttgacgatca gcaggcgttc cttgcggcgc taatggatga acaaacacta
    11221 acgcagctgg aacagctcaa gcagaatctg gaaaaccagc gccgtcaggc gcaaacgctg
    11281 gtcactcaga cagcagaaac gctggcacag catcaacaac accgacctga cgggttggct
    11341 ctcactgtga cggtggagca gattcagcaa gagttagcgc aaactcacca aaagttgcgt
    11401 gaaaacacca cgagtcaagg cgagattcgc cagcagctga agcaggatgc agataaccgt
    11461 cagcaacaac aaaccttact gcagcaaatt gctcaaatga cgcagcaggt tgaggactgg
    11521 ggatatctga attcgctaat aggttccaaa gagggcgata aattccgcaa gtttgcccag
    11581 gggctgacgc tggataattt agtccatctc gctaatcagc aacttacccg gctgcacggg
    11641 cgctatctgt tacagcgcaa agccagcgag gcgctggaag tcgaggttgt tgatacctgg
    11701 caggcagatg cggtacgcga tacccgtacc ctttccggcg gcgaaagttt cctcgttagt
    11761 ctggcgctgg cgctggcgct ttcggatctg gtcagccata aaacacgtat tgactcgctg
    11821 ttccttgatg aaggttttgg cacgctggat agcgaaacgc tggataccgc ccttgatgcg
    11881 ctggatgccc tgaacgccag tggcaaaacc atcggtgtga ttagccacgt tgaagcgatg
    11941 aaagagcgta ttcctgtgca gatcaaagtg aagaagatca acggcctggg ctacagcaaa
    12001 ctggaaagta cgtttgcagt gaaataacta ttcagcagga taatgaatac agaggggcga
    12061 attatctctt ggccttgctg gtcgttatcc tgcaagctat cactttattg gctacggtga
    12121 ttggtagccg ttctggtagt tgtgatggtg gtatgaaaaa agtcatttta tctttggctc
    12181 tgggcacgtt tggtttgggg atggccgaat ttggcattat gggcgtgctc acggagctgg
    12241 cgcataacgt aggaatttcg attcctgctg ccgggcatat gatctcgtat tatgcgctgg
    12301 gggtggtggt cggtgcgcca ataatcgcac tcttttccag ccgctactca ctcaaacata
    12361 tcttgttgtt tctggtggcg ttgtgcgtca ttggcaacgc catgttcacg ctctcttcgt
    12421 cgtacctgat gcttgccatt ggtcgcctgg tttccggctt tccgcatggc gcattttttg
    12481 gcgtcggagc gatcgtgtta tcaaaaatta tcaaacccgg aaaagtcacc gccgccgtgg
    12541 cggggatggt ttccgggatg acagtcgcca atttgctggg cattccgctg ggaacgtatt
    12601 taagtcagga atttagctgg cgttacacct ttttattgat cgctgttttt aatattgcgg
    12661 tgatggcatc ggtctatttt tgggtgccgg atattcgcga cgaggcgaaa ggaaagctgc
    12721 gcgaacaatt tcactttttg cgcagcccgg ccccgtggtt aattttcgcc gccaccatgt
    12781 ttggcaacgc aggtgtgttt gcctggttca gctacgtaaa gccatacatg atgtttattt
    12841 ccggtttttc ggaaacggcg atgaccttta ttatgatgtt agttgggcta gggatggtgc
    12901 tgggaaatat gctaagtggc aggatttcag gacgttattc accactgcgc attgcagcag
    12961 tgactgactt tataattgta ctggcactgc tgatgctctt tttctgcggc ggcatgaaaa
    13021 caacgtcgct tatttttgct tttatttgtt gcgcgggatt atttgccctt tcagcacctc
    13081 tgcaaatatt gttactgcaa aatgccaaag gcggagagtt attaggtgcc gcaggtgggc
    13141 aaatagcgtt taacctcggt agcgccgtcg gcgcatattg cggaggtatg atgctgacgc
    13201 tggggctggc atataattac gtggcgctgc ctgccgccct gctttcgttt gctgcgatgt
    13261 catcgttgct gctgtatggt cgctataagc gccagcaagc ggcggatagt ccggtgctgg
    13321 cgaaaccact ggggtagatt actgtcacgg tgtgcgttgc ttattgccgg atgcggcgtg
    13381 aacgccttat ccgccctacg cggttctggc acattttgta ggcctgataa gacgcggcaa
    13441 gcgtcgcatc aggcatgatg cgccaattgc ctgggttttt tactcttgtg gccataacca
    13501 cgcagcgccg cgtacgccgc tggaatcacc gtgcttcgcc ttacgcaccg gcgtttcaca
    13561 ttcgccgccg aagacaaatt gtttaatcaa ctgcggaacc gtttgatata aacggtctac
    13621 attgctcatc ccgcccccca ggacaatcac atccggatcg agaatattca cgacatgtgc
    13681 cagcgatttt gccagccgca gctcgtagcg acgcaatgcc agttccgcta ccggatcgct
    13741 ttcttcaacc aggcggataa tttcactgcc tttcagcgca tgtccgctca aacgacgata
    13801 atccgtcgcg aatcccgtgc ccgaaataaa ggtttcaata caaccttgtt taccgcaata
    13861 acaagggact tcctcgcgat aacgcagttc gtcttcgtcc atccacggta gcggattgtg
    13921 tccccactca cctgccgtgc cattgccgcc gatatgcgcc cgcccattga atgccacgcc
    13981 cgcgccgcat cccgtgccga taatcacggc aaataccgtc tgcgctcccg ctgccgcgcc
    14041 atctactgct tctgaaaccg ccagacagtt ggcgtcattt gccagccgca cttcccgctg
    14101 caacctcgcg cttaagtctt tatcgaatgg ctgaccgttg agccaggttg aattggcatt
    14161 cttcaccaca ccggtgtaag gcgaaattga gccaggaatg cccataccta ccgttccgcg
    14221 ctgccccgtc gcctgctccg ccatatcaac caacgtggcg atcgtttcaa tagtctgccg
    14281 gtaatcatca cgcggcgtgg gcagacgatg gcggtacaac tgctcccctg catcgcccag
    14341 tgcaatcact tcagttttgg tgccgcctaa atcgatacct atacgcacgg tactctcctt
    14401 atttttttca atatcaatag cgtagagacg gacaaccgga ttggcaatgc aaggccgccg
    14461 acaattcgtt atcatgcccg ctaaatttaa cgacaaggcc gtggaaatta tcatgctgtg
    14521 gttcaaaaat ttaatggttt accgtcttag ccgcgagatt tcgctgcgtg cagaagagat
    14581 ggaaaaacag ctagcctcga tggcatttac cccatgcggc agccaggaca tggcgaagat
    14641 gggctgggtt cctccgatgg gatcgcacag cgatgcgtta acgcacgttg ccaatggtca
    14701 aattgttatc tgcgcgcgca aagaagaaaa aatcctaccg tctccggtga ttaaacaggc
    14761 gctggaagcg aaaatcgcca aactggaagc ggaacaggcg cgtaagctga agaaaaccga
    14821 aaaagattcg ctgaaagacg aagtgctgca ctctctgttg ccgcgtgctt tcagccgttt
    14881 tagccagaca atgatgtgga tcgacacggt taacggtttg attatggtgg actgcgccag
    14941 tgccaaaaaa gcggaagata cgctggcact gctgcgtaaa agcctggggt cgttaccggt
    15001 tgtaccattg agcatggcaa acccgattga actgacgctg accgaatggg tacgttccgg
    15061 tagtgcggca cagggcttcc agctgcttga tgaagccgag ctgaaatcgt tgctggaaga
    15121 tggcggagtg atccgcgcga agaaacaaga tctgaccagc gaagagatca ccaatcacat
    15181 tgaagccgga aaagtggtga ctaaactggc gctcgactgg cagcagcgca ttcagtttgt
    15241 gatgtgcgac gatggttcgc tcaagcgtct gaagttctgc gacgagctgc gcgatcaaaa
    15301 cgaagatatc gaccgtgaag atttcgccca gcgttttgat gccgatttca tcctgatgac
    15361 tggtgaactg gcagcgttaa ttcaaaacct gattgaagga ttaggtggcg aagcacaacg
    15421 ttaattgctg attttccttt aatgccggat gcgacgcctg ccgcgtctta tccggcgtac
    15481 gaagccacac caggcatata attattcgct acggcgggca ataatttttt agcgcagcaa
    15541 tattatgcgt tttacgccgt aactgatcta atcgcctcat ccctattcgc ctgtcagcag
    15601 cacgcagaat attcactaac ccccatttat cgctggtaaa ttgttttgtt aacaactccg
    15661 cttttggggt gtcaatatat tcacgcaata aatcagaaat tgaacaaata tccgtcaggt
    15721 aaggatacca gcaagtttca ccgtgaaaat tacgcactcc accgttgcct gcaataaagt
    15781 ctttcgggta atcccagatc acgtttttat ccaacgtgat ccaataacgc ggtaaatcgg
    15841 tgcttccacc attttggctg cgcatgggat aacgggtaca gtgaatttgc aggttgatcg
    15901 ttggcgtcag taagtcatag atctcccgct gtaacttgct ccaggggcgt tgtgtcattg
    15961 tttttcctca agccggtgac gcgcttcatt gcgaattttc cgcgttgccc cggttttcac
    16021 taaatcccgt aactgcgaaa gatcgggttt atcgacaaat aaaagtaatg atggatcctc
    16081 ttttagccac accgttttca catcggcagc cagctgtgga ttattaatag ccagcgaccg
    16141 atcctgagac tcagtcaatg tcgttaacaa cgacgccggg gtatgtgcgt tgcgtaatgc
    16201 cgtttggcgc aaatgatcat tgcgactttg cagcaatgca cgccaccatt catcatctga
    16261 tttatctttt atcgtcgcca gttccgcaaa aatatcgcta tcctcatggt tccagcggtg
    16321 taatcctctt tcactttgtc tacgggtgct atttttcata ctgacaacag ccagcgccca
    16381 gtcagagttg aagcgatact gttgatcgag tttgttgata attccggaag gtaattcttt
    16441 attatcaaat aattggttac gcattcgata gcggtattca gcgggtaaat caaccagtaa
    16501 atcaatggca ttatttaaca tctcttcgtc aacaacacca ttatcaatca gcgccagctg
    16561 tacttcgtag gcttgctctt gatcttcttg ccgtttgctc atcattaaat ttaatgtgcg
    16621 ttgccataac tgtttcttat attccagcgg caataattta tcgcgtgcca gctcatccca
    16681 gacggtggga acatcatgct gagtcaatag ataactaatc acgtccttcg atgtcagata
    16741 acgcgcacct tcgcgcaaat tgtgcacatc ttcttttgcc agagacagtt gacgcatctc
    16801 cggcaacgct atcaataaca cctttacgat atttttattt tctttattat ccagatacat
    16861 ttgttctgcg atacgttcaa tatcctctgt acgcagggtt gctgacagtt gggtaatttt
    16921 taattcagta agcgtccgag ctaatgcttc acgaactttc agtgatttat ctttactcaa
    16981 catcactgct tgttgtgcgg ttaaatctgc tccggtaatt ctttcacgta actccgggtc
    17041 ttcgttattt atcaactcat cggccactgc agtcggaacc agccaggcgt aatcgtaatg
    17101 gctgagtaat gtcttgcgta cactgagaaa ggggctatga cttaacgacg caacaatatc
    17161 tggagaaaga ttatcctgtc tggcctgctc cagctgtacg ctctcgacgg gtacagacat
    17221 taatttttta ttcacatcgg cggaaatatt actgttttta accagccctc tggcaacagc
    17281 ctcatcttta attaattcat cggtaagcca atgcatctgt tgagcgggcc aggactgcgc
    17341 catcgattca cgcgtctggt catcggggtg ctgtaacagt tgcagcataa cggcgggtga
    17401 taaactctct gtatgagtca gaaaacgtgc caccagtgga tcttgatgct ttattttatt
    17461 cgctgggaaa ttatcgagtt tccccgtttc aatccatttt aatttctcat taatttcagg
    17521 agcggcttta tctcgaaaaa gcggtaaacc ctgaacgatg ttgtctgcga cgctatcata
    17581 gtcaggatac gcattggaaa tgttatcggt ttcgatttca gtgtgatcat aaagatcggc
    17641 agtcaccagt gaataggctt tttgaatatt ctcatcccag ttatatcctt caccacttcc
    17701 atcttcattg atataaaatt cagctttaat gacttcaaat tgcgttgctt tatcggaaac
    17761 aataatgcct cgcgcactaa ttcccttcat cacttcaaag ttatgatcat tgtaatcagc
    17821 ataaactaaa cctttcgctg tcatctgtcc tttgacatac gcgaaatcat gattaataaa
    17881 gttatcgaca ataacattac ctaatacaat cagatgacca ttacctgaca gataatcatc
    17941 ataatcgccg gaaatcgtta aattaccgtt caccaccagt ttatttttat tgctgaagtt
    18001 agcgggtaat gataggtcac cattatgata ataccagcgg atattatctt ttagcatata
    18061 agcagcttcc atactatcgt cggttgccgc tttaaccacg ttgcgctcat ctgccgtcat
    18121 aagatgaaag tcagaggtga taaggtcctc gctactgatc cactctgtgg cacatgcatt
    18181 gccagtcata aaaaataaga ccatacagat gaccaatata ttaattctat tcataatctt
    18241 caatgatatt tatatcaacc ttgatgaatt atgaatatat cggaaattga aaaaatcgca
    18301 gcaaaggaaa tattattatg gggttatgag aggacatttc agaaaaatgc cctcccgttc
    18361 aagcggaagg cgaggaatta cagatagcgg cacagataag aggtgggttc ggcaacttgc
    18421 agatgaaact cactgtgacc gggaacatta aacaccgaac cggcttcata cacttgccag
    18481 tcggtcgcgt caggcagtaa cacattcagc gcgccactga ttaccgtcat ctcttccggc
    18541 tcagcggtgc tgaaggtgta ttcgccttca accataacac ccacgctggc gcgaccagtg
    18601 ctgctgctgg aaaagccgat cgatttcact ttgccggaaa agtactcatt actttgaagc
    18661 ataaactggc cctgtctggt agttgaatat ctacttcact ataggggcca gatgcgctgg
    18721 ctgtcacgca aaattacacc agtaattccg cagccagccg tgcaatcaat acgttagaga
    18781 gcaagacagg aacatcgagc tgtttttgca gtaaatcgcg atgacgctgg ttaaatccca
    18841 aacaatccag catgatgaca tctgcgcctt tcgccagtaa ttctttcccg gcatcaatga
    18901 ttttttgttc tgaatcatga atggggttac ccaatgaaaa tacaggcggt ttctgcaaaa
    18961 tttgccattt ttgcgcctga acggtcagca actcctcaac cggtacgata acccccacct
    19021 gatgatcttc aacaatagag gaaaccagcg gtggcaatat ccgcgacggc tcaagaaaga
    19081 tcgtattacg cgcagtcata ctactaatgt ttgccgtact cattaatata atgacgtcgt
    19141 agccctgatt atcgagcact tcaaccacac cttgcaggtc acgctctact ttgcgacgcg
    19201 aaacatgggc cagatggttg tcatttaata atgtgagaat tgtgtcttcg cctgcttctg
    19261 gcgcgtactc cgccatcact tcttcgcggc ttaacttccc cagcaggcta tgatgggaaa
    19321 tattatcttc gtcaatgtat tctgtcagga gcggcaaaac ttcctgcata ggtacaatgc
    19381 cgatggttag aatcgccaac gacgcactca ttgttcttcc accttacttt tatttcgtcg
    19441 ttaattaccg ggcaagtgtt aagcaccact gctgacactt acttgtgcta caaatgacaa
    19501 aaagcgtagc agcagacgtg cacggcataa tgtaaagatt ccaaatgatt ccagtaatgg
    19561 attgttattg tttaatattc taattatttg aaaaacatga attatgaaaa atgtgacgca
    19621 gatcccttgt agtggcactc agaatccctt ccgagcagtc tgatgcggtc actctttgtc
    19681 agggtcctca tagcgttttt tcgcatccat cgcttcctga gcggtcggat gttcactgat
    19741 caacgagtct ggtttaggat gatcggctct gagttggtac caggttaccg tctgtcctgg
    19801 ctttcctttt tcaattgtca ctatatatgc ttcacgagga taaggcggtt tcgttggcat
    19861 cgttcttcct tatttcacgg gatgaacgtt aagtataggc gctcgaaaat caacaattga
    19921 tcgtctgcgc cagggcgctg cgaatttcag aaatcacctg gctgggttcg tttgttgcgt
    19981 cgatgataat atgcgcaact tcgcgatata gcgcatcgcg ttcttccagc acttcctgaa
    20041 cttcttcgct cagcggtttt cccgttaagg ttggccgtaa atcttcttcc ggtgcagctt
    20101 gcagtcggtt aaccaggact gatactggcg cacacaaata aaccacgatc ccgttatttt
    20161 gcatgaagtg acgattaaat tccgtcagaa taatgccgcc gcctgtagcg ataacggtgg
    20221 atggcgcagt taccgcttcc agcgccgccg tttctctggc gcgaaatccc gcccactctt
    20281 ccctttcgac gatctccgcg accgtcatat tgagctgtga ttgcaaccac tgatcggtat
    20341 cgacaaaccg acggttaagc gaatcggcaa gggccattcc gaccgttgtt ttaccacagc
    20401 cccgaggccc gatcagaaaa agaggttgtg tcatcgtggg ttttccccaa taggtcgcaa
    20461 tgcggcgaaa gccggtgtca tgagaatagc gatcatacca tcaaactagt acaatttcga
    20521 ttgtaaagaa aaaattccac ttaaagtgaa aatctcaata catcccttac tataccaata
    20581 aatattcaag aatgaagtgt aaataataaa ttacatttag ccacgactac gttgcacttc
    20641 cagccaccac ttctccagct ccgcggcaaa ggcctggcgg tcacgttgtg aaaggctatc
    20701 tggtccgccg gtctggatcc cactggcacg taaggtatcc ataaaatcgc gcatcgtcag
    20761 gcgctcacga atggtcgctg gcgtgtaacg ttcaccgcgc ggattaagcg ccgcagcgcc
    20821 tttctcgatg gcttcagcag ccaaaggtat atctgcggtg atcaccaaat ctcccgcttc
    20881 acactgccgg acaatttcgt tatcggcaac gtcgaaacct gccgcgacgc gcagcgtacg
    20941 aataaatcgc gatggcggca cgcgtaaact ctggtttgct accagtacca gcggcatctg
    21001 catacgttcc gccgcgcgat acaaaatctc tttaattaca ttgggacacg cgtcggcatc
    21061 cacccaaatt gtcataaagt catcctttgt tgggtaatcc tctattgtgt cgcgcttttg
    21121 ccttccggca tagttctgtt tatgcttctg ccagcgatta tcaaaacaat gaatttcacg
    21181 gcaggagtga ggcaatggaa aagaaaatcg gttttattgg ctgcggcaat atgggaaaag
    21241 ccattctcgg cggtctgatt gccagcggtc aggtgcttcc agggcaaatc tgggtatata
    21301 ccccctcccc ggataaagtc gccgccctgc atgacaagtt cggcatcaac gccgcagaat
    21361 cggcgcaaga agtggcgcaa atcgccgaca tcatttttgc cgccgttaaa cctggcatca
    21421 tgattaaagt gcttagcgaa atcacctcca gcctcaataa agactctctg gtcgtttcta
    21481 ttgctgcagg tgtcacgctc gaccagcttg cccgcgcgct gggccatgac cggaaaatta
    21541 tccgcgccat gccgaacact cccgcgctgg ttaatgccgg gatgacctcc gtaacgccaa
    21601 acgcgctggt aaccccagaa gataccgctg atgtgctgaa tattttccgc tgctttggcg
    21661 aagcggaagt aattgctgag ccgatgatcc acccggtggt cggtgtgagc ggttcttcgc
    21721 cagcctacgt ctttatgttt atcgaagcga tggccgacgc cgccgtgctg ggcgggatgc
    21781 cacgcgccca ggcgtataaa tttgccgctc aggcggtaat gggttccgca aaaatggtgc
    21841 tggaaacggg agaacatccg ggggcactga aagatatggt ctgctcaccg ggaggcacca
    21901 ccattgaagc ggtacgcgta ctggaagaga aaggcttccg tgccgcagtg atcgaagcga
    21961 tgacgaagtg tatggaaaaa tcagaaaaac tcagcaaatc ctgatgactt tcgccggacg
    22021 tcaggccgcc acttcggtgc ggttacgtcc ggctttcttt gctttgtaga gcgccaaatc
    22081 tgccgatttc aaccactcgc gatagtgact catttgtggg ttcagcggcg caacccccac
    22141 actaatccgt aaatttacct gtggcgtatt cggcaaacgt aatgtattta gcccttcatg
    22201 cacccgtaac atggcggtaa tggcgctctc agctggcgta ccggacatga ttaccgcaaa
    22261 ctcatcgccg ccaaaccgac caatcacatc gctaccgcgc agggtaattt gtaactgtcg
    22321 ggtaagcgcc acaatcgctt catcgcccac atcatggccc caggtatcgt tgatgctctt
    22381 gaaatggtcg atatcgataa tcagtaacgt tgcatcgcga ttatgccgcc gacagttatc
    22441 aaattcattg cgtaacatag tttcccaatg acgtcggtta tacacgcccg tcatgccgtc
    22501 gcgggtactc atgacctgca acctgcgttt atgttccgcc agtttggttg ccgtctggta
    22561 gctgacccag ccaaacagca gaggataaat gacaataatg ggaagggaga gccaccattc
    22621 cagcggcgca ctattgaacg acacggtaat gcccgtcagc tcgagggtga caaggcagga
    22681 aaccaccatc aacaccagac ccgcgacaaa cagacggggg ccgcctgccc ccatcaaatt
    22741 cagacacata atcatcaaca tcgcggtgga aggcagcacg tttacgccca ttacgcctac
    22801 ccatattccc gctaatactg catcggtttt taagttgtaa atttcccggc taagcggatc
    22861 gacggccctg ctcgctatct gccaggctaa atgcggccag acgaacgccc agccgaccaa
    22921 caccagccac caccagcccg gcggcgggtg tgaaaccagc gttgaagcaa tcggtaagaa
    22981 catgccagcc aggccaaccg cacggggtag tctgacgcga cgggcgaagc gcagcccgga
    23041 ccgctgatgt tcgttttgag gagttaaagg aggttcctcc ccgtgcgccg ccgctttttt
    23101 gaaaaagttt tcatcattca ttatttttgg gaacattcag aaacaatttt cccaaattat
    23161 agagacgggg ctaaaggcag aagtatgaaa tttcgggtga gccattttag tagctcaccc
    23221 gttagcacta tcaggtatca ggcggctttc ttgaggcagg cgctcataaa cttattacga
    23281 tcatcacctt tcagtgattg ttgcgttgct tgattattac attcgcgcat cttttgctgc
    23341 tgtggcgtca aacttttttc accaggcgca gacttgctgt tcttcaggca atcactcatg
    23401 taggtcttac gagcatcccc tttcaacgcc tgtgccgtcg cctgctgatt acaggaggtc
    23461 atacgctgtt gttgtggggt taaggttctc tcggcagcgc cgacggtggt taaaaaaacc
    23521 agaccgaaaa gcaaggtaac cagtaatgta attttcatag caccatccct cttcatgttt
    23581 taaccatgag cgtatgcgcc cgtgatctga cattaagtct ggttgctaac agcaaaaaaa
    23641 ccacccggca gcgaaaattc actgccggga gcggttttat ttcagcccca gagcggcttt
    23701 catggtgtag aagagatcgg tctggtcggt cagtccaacg acattggcgg catgcgggcc
    23761 atacgccgca atacgcaact gactgccggt atgttcttgt gaatcctctt cggagttccc
    23821 gtaactcatc accatcactg cgccatcttt ggtatttagc gcctgagtga ggcccggagc
    23881 tttggtatcc ggcgcaacaa tctggctggc gtgggcgtga tcagcggtga ctatgaccag
    23941 cgtgttacca tcctttttgg cgaattccag cgcccgttgt acggcttcat cgagatcgac
    24001 cgtctcgcca atttgcccac aaggattcgc agcatgatcc tgtttatcga ttgacgcacc
    24061 ttcaacttgc aggaaaaagc ctttctcatt tttactcaac aattcaatgg ctttgtcggt
    24121 catctgtgcc agggtcggta cactgtcatt acgttgcgga ttaggcgtac aggtgactgc
    24181 gggcttatcg atattaccgt ggtacgttgc tttcggtcct agccagcgca ctggcatatt
    24241 gccgtcagca aacaggccaa gcaggggttt ttgctgattc gcttccgtca ccgaattcag
    24301 tgaggcagca tcgctcacca actgataacc acgcgcctgt gcctgttcac gcagcgtttt
    24361 tccctgccat tcaccagcgg ttgccgtttc agcaaaggtt tttgcgccgc cgccaagcgt
    24421 aacgtcggca cgagcgttaa gcagctgttc ggtaatcgat ccttttccgc ctttttccag
    24481 agcgttaccc ggacattttt cactggtcgc gctcggaccg tagcatttgc gcgaggtcac
    24541 atgcgccacc agcgcggcgg gcgtggcatc ctgcaactct gcggtagaaa cgttaccggt
    24601 cgccagacct gcggcttttg ccatttccag aatcgttggg tgatcttttt cgtgaatatc
    24661 gacgcccagc gcgccgttat aggttttgac accggttgac caggcggttg ctgatgcagc
    24721 cgagtcggtg acgtagtccg gttttccggt ttttttattc agcgcatagt gagtgtattg
    24781 cccggtaagc ggtaaggcat cgataccttt aaaaaagccg cccgcacctt cggcataatt
    24841 acgtgcggca gtaatttccg aatcccccat cccatcgcca atcagcaaaa taatattttt
    24901 tgcaggttta tcgctaagag aatcacgcag agcggcggtc tgatcagccg ttaagcggcg
    24961 agcaccgccg ggtgcagtaa tatcgccctg agcagcccgg ttttccagaa caggcatttc
    25021 tggtgtccgg gcttttgtca caggggtaaa cagtaacggt aagagtgcca gtgcaatagt
    25081 gctttgtttc actttatttt ctccatgtac aaatacatta aaaaataaaa acaaagcgac
    25141 tataagtctc ggccgtgaca actttatgac agctgttgaa aagattaact ttttactgac
    25201 gaggatgctt caataacttc tttacgtaat cgcgcagcag ctccgtatcg tcgtcaggaa
    25261 tgctggcatc gggctttacc tcgtacagcg ccccctctac ctgatcaatc aaccgctgtt
    25321 ggtcattttg cgccatattg cgaagcattg cagtgacgat aatctccaaa gcttctacct
    25381 gcgcacacag ttctttcgac tcttcttctt tttgggcaag cttaaataac aactcagcaa
    25441 tgagattttt catgtctgta tttccttatc caaagtatgg agaagttaac attatgtttc
    25501 a
//
LOCUS       DAZFAB010000065        25468 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZFAB010000065 DAZFAB010000000
VERSION     DAZFAB010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.065"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            293..1498
                     /gene="gltS"
                     /locus_tag="ACHJWA_002982"
     CDS             293..1498
                     /gene="gltS"
                     /locus_tag="ACHJWA_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFO4309533.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            1501..2367
                     /locus_tag="ACHJWA_002983"
     CDS             1501..2367
                     /locus_tag="ACHJWA_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4309534.1"
                     /translation="MKKRRKEPETLREHCRHIFGDEPPVLCVWETEFDYADAELKALA
                     AKEWQQISEWDLSAYYVLNLVYNEPMQIELFRYLFPLCLAQWHETVLAGGYGDHFEES
                     LMKALCRPYLWQEMMNASQRQQVRQFLLDTALQRMDNERGFNNVLCWLAVFNTLGGAA
                     PLIRSLWSRWWALDTPGKAVCAIQYAAHLIYPIEANPLWSQEWIGWGHPLGHKDGWSS
                     DNRAFLRQMLTPEMIVAGVQAAAEILRGEPEGAMAARIAQDAYEAMDILTIQIEDLLR
                     DLSCDESGHALE"
     gene            complement(2352..4433)
                     /gene="recG"
                     /locus_tag="ACHJWA_002984"
     CDS             complement(2352..4433)
                     /gene="recG"
                     /locus_tag="ACHJWA_002984"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFO4309535.1"
                     /translation="MTGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(4439..5128)
                     /gene="trmH"
                     /locus_tag="ACHJWA_002985"
     CDS             complement(4439..5128)
                     /gene="trmH"
                     /locus_tag="ACHJWA_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFO4309536.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(5135..7243)
                     /gene="spoT"
                     /locus_tag="ACHJWA_002986"
     CDS             complement(5135..7243)
                     /gene="spoT"
                     /locus_tag="ACHJWA_002986"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFO4309537.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(7262..7537)
                     /gene="rpoZ"
                     /locus_tag="ACHJWA_002987"
     CDS             complement(7262..7537)
                     /gene="rpoZ"
                     /locus_tag="ACHJWA_002987"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFO4309538.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(7592..8215)
                     /gene="gmk"
                     /locus_tag="ACHJWA_002988"
     CDS             complement(7592..8215)
                     /gene="gmk"
                     /locus_tag="ACHJWA_002988"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFO4309539.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRDQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            8355..8612
                     /locus_tag="ACHJWA_002989"
     CDS             8355..8612
                     /locus_tag="ACHJWA_002989"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4309540.1"
                     /translation="MIYCNKDDSQEASSQTPFLPLGFAFTTLYICQPDYSGGEDESMD
                     GDINKYLVLAIICVGGLSGLVASQSTGRNFPPATANKTVGR"
     gene            8524..10155
                     /gene="ligB"
                     /locus_tag="ACHJWA_002990"
     CDS             8524..10155
                     /gene="ligB"
                     /locus_tag="ACHJWA_002990"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFO4309541.1"
                     /translation="MWAVCPAWSPARAQEEIFRLQQQIKQWDDDYWKEGKSEVEDGVY
                     DQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRKMADKNALSLWMRERSDL
                     WVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSLISAVPQTVSGPLANSTL
                     QGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSLGVFVWAWPDGPQLMTDR
                     LKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVTDGVVVRGAKEPESRHWL
                     PGQAEWLVAWKYQPVAQVAKVKAIQFAVGKSGKISVVASLAPVMLDDKKVQRVNIGSV
                     RRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTPPENRFNSLTCYFASDVC
                     QEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFSWLLLTPEQLQNTPGIAK
                     SKSAQLWHRFNLARKQPFTRWVMAMGIPLTRAALNASDERSWSQLLFSTEQFWQQLPG
                     TGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(10152..10769)
                     /locus_tag="ACHJWA_002991"
     CDS             complement(10152..10769)
                     /locus_tag="ACHJWA_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFO4309542.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11061..11885)
                     /gene="dinD"
                     /locus_tag="ACHJWA_002992"
     CDS             complement(11061..11885)
                     /gene="dinD"
                     /locus_tag="ACHJWA_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFO4309543.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAAVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            complement(12106..12969)
                     /locus_tag="ACHJWA_002993"
     CDS             complement(12106..12969)
                     /locus_tag="ACHJWA_002993"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFO4309544.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            13096..13812
                     /gene="rph"
                     /locus_tag="ACHJWA_002994"
     CDS             13096..13812
                     /gene="rph"
                     /locus_tag="ACHJWA_002994"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFO4309545.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            13878..14519
                     /gene="pyrE"
                     /locus_tag="ACHJWA_002995"
     CDS             13878..14519
                     /gene="pyrE"
                     /locus_tag="ACHJWA_002995"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFO4309546.1"
                     /translation="MKPYQRQFIEFALGKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGLEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(14556..15152)
                     /gene="slmA"
                     /locus_tag="ACHJWA_002996"
     CDS             complement(14556..15152)
                     /gene="slmA"
                     /locus_tag="ACHJWA_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFO4309547.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(15259..15717)
                     /gene="dut"
                     /locus_tag="ACHJWA_002997"
     CDS             complement(15259..15717)
                     /gene="dut"
                     /locus_tag="ACHJWA_002997"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFO4309548.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(15695..16915)
                     /gene="coaBC"
                     /locus_tag="ACHJWA_002998"
     CDS             complement(15695..16915)
                     /gene="coaBC"
                     /locus_tag="ACHJWA_002998"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFO4309549.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            17087..17755
                     /gene="radC"
                     /locus_tag="ACHJWA_002999"
     CDS             17087..17755
                     /gene="radC"
                     /locus_tag="ACHJWA_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFO4309550.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            17972..18208
                     /gene="rpmB"
                     /locus_tag="ACHJWA_003000"
     CDS             17972..18208
                     /gene="rpmB"
                     /locus_tag="ACHJWA_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFO4309551.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            18229..18396
                     /gene="rpmG"
                     /locus_tag="ACHJWA_003001"
     CDS             18229..18396
                     /gene="rpmG"
                     /locus_tag="ACHJWA_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFO4309552.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            18494..19303
                     /gene="mutM"
                     /locus_tag="ACHJWA_003002"
     CDS             18494..19303
                     /gene="mutM"
                     /locus_tag="ACHJWA_003002"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFO4309553.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(19342..19821)
                     /gene="coaD"
                     /locus_tag="ACHJWA_003003"
     CDS             complement(19342..19821)
                     /gene="coaD"
                     /locus_tag="ACHJWA_003003"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFO4309554.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(19829..21106)
                     /gene="waaA"
                     /locus_tag="ACHJWA_003004"
     CDS             complement(19829..21106)
                     /gene="waaA"
                     /locus_tag="ACHJWA_003004"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFO4309555.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            21519..22577
                     /gene="rfaQ"
                     /locus_tag="ACHJWA_003005"
     CDS             21519..22577
                     /gene="rfaQ"
                     /locus_tag="ACHJWA_003005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFO4309556.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            22574..23698
                     /locus_tag="ACHJWA_003006"
     CDS             22574..23698
                     /locus_tag="ACHJWA_003006"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO4309557.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYHLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            23691..24488
                     /gene="rfaP"
                     /locus_tag="ACHJWA_003007"
     CDS             23691..24488
                     /gene="rfaP"
                     /locus_tag="ACHJWA_003007"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFO4309558.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVHDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            24531..>25468
                     /gene="waaO"
                     /locus_tag="ACHJWA_003008"
                     /gene_synonym="rfaI"
     CDS             24531..>25468
                     /gene="waaO"
                     /locus_tag="ACHJWA_003008"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008918 - lipopolysaccharide
                     3-alpha-galactosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HFO4309559.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHVFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDV
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKH"
BASE COUNT         6095 a         6192 c         6756 g         6425 t
ORIGIN      
        1 tggaaacaga catcgaaaac aatcccgtgg tagcaaagcg ggcattttag ctgaccgaaa
       61 gagaaaagca aacgtttgcc acatcctcat ctaaatattt aaatgagata aatgcacttt
      121 tttatataac ttttggtatt tttctgctta aaatccatgc catttgttca ttaacagctg
      181 agaatttgac ccaggaaagc ataactcgat acccgtcata gttcatgaag ttgccactgc
      241 gcgatgaagt atgacgagta tgaaagagtg atgcggatac aaaggagtaa ctatgtttca
      301 tctcgatact ttagcaacgc ttgttgccgc aacgctgacg ttgttgctcg ggcgtaagtt
      361 ggtccattcc gtctcctttt tgaagaaata caccataccg gaacctgttg cgggtggttt
      421 gttggtggcg ctggcgctac tagtactgaa aaaaagcatg ggctgggaag tcaactttga
      481 tatgtccctg cgcgatccgt taatgctggc tttcttcgcc accattggcc tgaacgccaa
      541 cattgccagt ttgcgtgccg gtgggcgtgt ggttggcatc ttcttgattg tggttgttgg
      601 tctgttggtg atgcaaaatg ccattggcat tggtatggct agcctgttag ggcttgatcc
      661 gctgatgggg ctgttggccg gttctattac gctttccggc ggtcacggta cgggcgctgc
      721 gtggagtaaa ttgttcattg aacgttatgg cttcaccaat gcgacggaag tggcgatggc
      781 ctgtgcaacg ttcggtctgg tgctgggcgg cttgattggc ggtccggtgg cgcgctatct
      841 ggtgaaacac tccaccacgc cgaacggtat tccggatgac caggaagtcc cgacggcgtt
      901 tgaaaagccg gatgtgggcc gcatgatcac ctcgctggtg ctgattgaaa ctatcgcgct
      961 gattgctatc tgcctgacgg tggggaaaat tgttgcgcaa cttttggctg gcactgcttt
     1021 tgaactgccg accttcgtct gtgtactgtt tgttggcgtg attctgagca acggtctgtc
     1081 aatgatgggc ttttaccgcg tctttgagcg tgcggtatcc gtgctgggta acgtaagcct
     1141 gtcgttgttc ctggcgatgg cgctgatggg gctgaaactg tgggagctgg cttcgctggc
     1201 gctgccgatg ctggcgattc tggtggtaca gaccatcttc atggcgttgt atgccatctt
     1261 cgttacctgg cgcatgatgg gcaaaaacta cgatgcggca gtgctggctg cgggtcactg
     1321 tggttttggc ctcggtgcaa cgccaacggc aatcgccaac atgcaggcga tcactgaacg
     1381 ctttggcccg tcgcacatgg cgtttttggt ggtgccgatg gtcggtgcgt tctttatcga
     1441 tatcgtcaat gcgctggtga ttaagctgta tttaatgttg ccgatttttg ccggttaaaa
     1501 atgaaaaagc gtcggaagga accggaaacg ctacgtgaac actgtcgcca tatttttggt
     1561 gatgagccgc cagtgctctg tgtatgggaa acggaatttg attacgcaga tgccgaactc
     1621 aaggcgctgg cggctaaaga gtggcagcag atcagcgaat gggatttgag tgcttactat
     1681 gtactcaatc tcgtttataa cgaaccgatg caaatagagt tgtttcgcta tctgtttccg
     1741 ctctgcctgg cgcaatggca tgaaaccgtt ttagctggcg gttatggtga tcacttcgaa
     1801 gaatccttaa tgaaggcatt gtgtcggccc tatctctggc aggagatgat gaatgcttcc
     1861 caacgccagc aggtacggca atttttactg gacaccgcgt tgcagcgtat ggataacgag
     1921 cgtggtttca ataacgttct ttgctggctg gcggtgttca atactctggg tggggccgcg
     1981 ccgttaattc gttctctctg gtcgcgctgg tgggcgctgg atacgccagg aaaagcggtg
     2041 tgtgctattc agtatgccgc gcatctaatt tatcccatag aagccaatcc gttatggtcg
     2101 caagaatgga taggctgggg gcatccatta ggccataaag atggctggtc ctctgataat
     2161 cgcgcttttc ttcgccaaat gttaacacct gaaatgattg tggctggcgt acaggcagcg
     2221 gctgaaattc tgcgtggtga accggaaggc gcaatggcgg cgcgaattgc acaggatgcg
     2281 tatgaggcaa tggatatcct cactatccag attgaagatt tactgcgtga cttgtcttgt
     2341 gatgagtctg gtcatgcgtt ggagtaacgt tccgtctccg gcatccagcg ttctatcagg
     2401 gcttttgctt gttgtgggta acgttcgtga atatgtcgcg ccaggcgctg aacttccggg
     2461 atcatcgcct gatcgcgcag taaatccgcc actttaaact cagcattacc cgtctgacgc
     2521 gtacctaaca gttcacccgg tccgcgaatc tctaagtctt tctgtgcaat cacaaaaccg
     2581 tcgttgctgt cgcgcagcac ttgcagccga atttgcgcgg ttttggagag cggcgttttg
     2641 tagagcagca cgcagtgaga agccaccgca ccacgaccta cacgcccgcg aagctggtgt
     2701 aactgcgcca gacccagacg ctcagggttt tcgataatca tcaggctggc gttaggcaca
     2761 tcaacaccga cttcaataac ggtagtggca actagcagat gtagctcacc ttgtttaaac
     2821 gacgccatca ccgcctgttt ctcggctggt ttcatccgcc cgtgtaccag gccaacattc
     2881 aattctggta gcgccagttt caactcttcc caggtagctt ccgccgcctg cgcttccagt
     2941 aactctgact cttcaatcaa cgtacaaacc cagtacgcct gacgaccttc agttatgcag
     3001 gcgtggcgca cgcggtcaat gatgtcggta cggcgggtat caggaatagc gaccgtagtc
     3061 actggcgtgc ggcctggcgg cagctcatct atcaccgagg tatcgagatc ggcatacgcc
     3121 gtcattgcca gcgtgcgggg gatcggcgtg gcggtcatga tcaactgatg cggatggaag
     3181 ccttgctgct ggcctttctc ccataacgcc agacgctgat gcacgccaaa gcgatgttgt
     3241 tcgtcgataa tcaccagcgc cagaccgtta aactgcacct gttcctggaa gatggcgtgc
     3301 gtaccaacaa tcatctgcac ctgaccgctg gcgatggctt cctgctgaga cagtcgcgct
     3361 ttgcctttct gcttaccggc gagccagccc acttcgatac cgagcggttc aaaccagttg
     3421 cggaagttat tggcgtgctg ttcggcgagt aattcggttg gtgccatcaa tgccacctgt
     3481 ttgccgtggg caatcgcacg cagcgcggcg agggcggcga ccagcgtttt accggaacct
     3541 acatcgccct gtaccaggcg catcatcggc acatccagcg ccatatcgcg ctcgatctcc
     3601 gccactacgc gtgcctgtgc gcccgttggc ttgaacggta aggctgcgag gagtttattt
     3661 ttcagcgcat cattggcgct cagcggctgg gcatgaaaac gctgtgctcc ggcacgtaag
     3721 gctaacatgc tgaggttgtg cgccagcagt tcttccagaa tcagacgacg ttgcgctgga
     3781 tgctgcccgg tttccagatc gctaagctgt agcgtcggtg gcgggcggtg caaagtgcgc
     3841 aacgcttccg gtagcgtcat cattccttgt gacagttccg gcggcaggag ttcttcaatg
     3901 gcgcaggtgt cgagcagatc cagcgcctgg tcggttaatt tacgcagcgt ggcctgcttt
     3961 acgccttccg ttgttggata aaccggcgtg agcgtttcct gtaattctgg cgtgctgaga
     4021 tcgccctgca cgcggtattc cgggtggatc atctccgcac catatttacc gcgctttgct
     4081 tcgccataag ccagtacacg gcggcccgtc gccaggctat ttttcattgc cgcgctgaaa
     4141 ttgaaaaagc gcatggtgag gatgccggaa ccgtcgctga tctggcaggt catcatccgc
     4201 cgaccgccga aggagatatt gcagttcagc acttcgcctt ccaccgtggc ataaacgccc
     4261 ggcagtagtt ctccgatggg gtagagatgg gtgcgatctt cgtagcgcag gggaaggtgt
     4321 aagagtagat cctgcacggt atgcaggttg attttcgcca gtttgttact aagtgctgcg
     4381 ccaacgcccg ttagggaact gagtgggaca gcatctaaca ggcgacctgt catggcactt
     4441 accctgcagc ctgcatagta gcccaccagt cggcatcagc ttcgatctcg ccttgctgat
     4501 tgacgtgggg ataaggcagg cctttgcgtt ttgcgacttt cgccagcacc ggatagccgc
     4561 cttcaaacaa caggcgttgt tgctctgctt ccggcaacat gctgttttca cgcaggtaca
     4621 tgcctgcatt ttgccgctga cgctgggctt cgtaaagaat gagggctgag gcaacggaaa
     4681 cattcagcga ctgcaccatg ccgatcatcg gaatgatgat gtcctgatcc gccagggcca
     4741 atgcttcctg cgtgatgccc gttttctcct gtcccatcaa aatgcaggtc gggcgagtgt
     4801 aatcaatttc gcggaaatcg acagcgttat cagaaagatg ggttgccaga atctgcatgc
     4861 cctggccttt gagatgagcg acggcatcgc caatggtgcg gtgtgttttc acctgtaccc
     4921 agctgttact acccgccgct gccgaagcca tggtgcgcat gcggctacca ggccagacgg
     4981 cgtgaacttc atgtacgcca acggcatctg cggtacgaat aatcgcagaa acgttatgag
     5041 gtttgtggac ctgctccatg cagacggtca gatcaggctg ccgcctggcg agcatttcgc
     5101 agatgcgtgc ataacgtgtt gggttcataa aacattaatt tcggtttcgg gtgactttaa
     5161 tcacgtctgg catcacgcgg attttgcgca tgatattcgc cagatgcaca cggtcacgag
     5221 cggtcagacg aataaaggcg ctgtagacgc gaccatcttt ctcttccgta ttcaaacttt
     5281 gaatattcga ggtcgtggtg ttaattgccg ccgtcaggtt tgccagcgca ccctgatgat
     5341 tgaacatctc caccttgatt tcggtgatga actcctgcgc cgtctcttta tcccattcca
     5401 cagccataaa cttctctggc tctttctggt agccacggat gttacggcag gattcatggt
     5461 ggatcaccag acctttaccg gggctgacgt gggcgataat cgggtcgcca ggaatggggc
     5521 ggcagcattt cgcaaaggtg atcagcacgc catcagcgcc tttgatcggc agatgcccgt
     5581 ggctttgagt cgccggtgga atggaggcgt ccccatgttg tagatttttc gcgaccacca
     5641 cgctcattgc gttaccaagg ccgatttctg ccagcagatc gtcaagcgtt gccagcttca
     5701 tgcgatccag ctcgcgctga atattttcct gcgggatttc attgagctta cggctaccac
     5761 ccaaagcatg gttgagcaga cgacggccca ggcttacaga atcatcacgc ttgaggtttt
     5821 tcagcaactg acgaattttg gcgcgcgctt tcgagctaac gacaaagttc agccaagcgg
     5881 cattcgggcg agcgcccgga gcggtaatga tttcaacggt ctgaccgcta gtaagcggct
     5941 gcgacagcgg gtaaggctgg cggtcgacgc gtgcgcccac gcaggcatgg ccgatatcgg
     6001 tatgcactgc ataagcgaag tcgacgggcg ttgcaccggc aggcagctcg acaatgcgcc
     6061 cttccggtgt gaaaacgtaa atctcatccg ggaagagatc ggatttaacg ctctcgataa
     6121 attcaaacga actaccggcg ctctgttgca gctccagcag gctttgcatc cagcgctggg
     6181 cgcggatttg tgcggtagta ctggtttcgc cgtgctcttt ataagcccag tgcgcggcaa
     6241 cacccatctc cgccatctga tccatatctt cggtacggat ctggacctca accggcacgc
     6301 cgtgcgggcc aatcatcgag gtgtgcaacg actgatagcc gttcgctttt ggaatggcga
     6361 tatagtcttt cacgcggccc ggacgcggct tgtacaggct gtgcatctgg cccagcacgc
     6421 gataacaggt gtcagaatca ttgacgatca cgcggaaagc gtagatgtcc atgattgagt
     6481 gaaaacgctg ctctttgagc accattttac agtaaatcga ataaagatgc ttttcgcgac
     6541 cactgacgcg gcacggtatt cccgcttcct gcaaacgccc ttcgatttca gaaagaatct
     6601 tctggatcat ctctttacgg ttgccgcgcg cggctttcac cacttctttg attacgcgat
     6661 aacggttggg atacagcgcc tcaaaaccca gctcttcgag ttcggtttta atgtggtgga
     6721 tacctaaacg gtgcgccagc gggctgtaaa tttcgagagt ttcacgggcg atgcggcgac
     6781 gtttgtccgg gcgaagtgag cccagcgtgc gcatgttgtg ggtacggtcg gcaagtttga
     6841 tgaggatgac gcggatatcc tgcaccatcg ccataatcat cttgcgaaag ttttcggcct
     6901 gcgcctcttt cttatcgcgg aacttgagtt tatcaagttt cgacaccccc tctaccagct
     6961 cggcgacgct tttaccaaaa agctgttcca tatcctggta ggtggcggga gtatcttcaa
     7021 tcacgtcatg cagcagcgcc gccatcagcg tttcatagtc gagtttcatc tcggccagaa
     7081 tgcaggcaac cgctaccggg tgcgtgatat agggttcacc gcttgaacgt gtttgcccct
     7141 cgtgagcatc acgtgcaacg agatacgcct gccgcagacg cttgatttgg tcttccggca
     7201 ggtaggtttg aatcagttga ttcaggcttt caaacagata caagggcgac ccgctttgtg
     7261 attaacgacg accttcagca atagcggtaa cggcttgtaa ttcagcggct tcctgctctt
     7321 gctgttcctg gcgttcgcga acgtcgagga tctggttgtt gatcagacct tcttcgattt
     7381 cgcgcagcgc gattacagtg gttttatcgt tttcttccgg taccagcgga tcctttccgc
     7441 ctacctgcat ctgacgagcg cgacgcgcgg cgaccagtac caggtcaaaa cggttaccaa
     7501 ttttctctac agcgtcctga acagttacgc gtgccatact taaaaagctc cacaggtgaa
     7561 gaaatgactg ggcatgatac tgaaatcagg ttcagtctgc caacaatttg ctgattaaag
     7621 cgtcatgacg ctgcttttgg cggctcatgc gcagacgttc ggcgcgaata atggtcttca
     7681 aatcggtcaa cgcggtatcg aagtcatcat tcacaatcag gtaatcatat tcggcgtaat
     7741 ggctcatttc tgcaacagct tgcgccatac gctttgcaat gacctcttcg ctgtcctgat
     7801 cgcgaccgcg tagacggcgg tccagttcaa ttttagacgg cggtaaaata aagatactcc
     7861 gcgcgtgcgg catcttctgg cgaatttgct gcgcgccctg ccagtcgata tcgagaaaaa
     7921 catcgacacc ggtcgccagt acttgctcaa tggcctcacg cgaagtgcca tagtaattac
     7981 caaaaacttc tgcgtgttcg aggaacgcat ctctgctaat catttcttta aattcatcat
     8041 gattaacaaa gaaataatgt tcaccgtgga cttcaccagg acgcggttgg cgtgtggtgt
     8101 gtgaaacaga aacctgggtg tcatacaacg gttgggtttt taataaagcc tgaatcaggc
     8161 tggatttacc cgcgccactg ggggcagaaa caatataaag cgtgccttga gccatgagta
     8221 tctttcgtat gtgattagcg aaataaagcc tacatacgag cttattatac acggcgcggc
     8281 catgtgacgt agcccttgct cacacttttt gcgcaaagtt gttgcgtttt gcgtagctgt
     8341 ttccgcattt gcctgtgatt tactgcaata aagacgattc tcaggaagcg tcctcgcaga
     8401 ctccgttttt gccactcggc tttgccttca ccacgttgta tatctgccag cctgactaca
     8461 gcggaggtga agatgaaagt atggatggcg atattaataa gtatcttgtg ctggcaatca
     8521 tctgtgtggg cggtctgtcc ggcctggtcg ccagccagag cacaggaaga aattttccgc
     8581 ctgcaacagc aaataaaaca gtgggacgat gactactgga aggaaggaaa aagtgaggtg
     8641 gaagacggtg tttacgatca gttaagcgcc cgtcttacgc agtggcaacg ctgctttgtg
     8701 agcgagcccc gcgacgtgat gatgccgcct ttaaatggtg cggttatgca tccggttgcc
     8761 catacgggtg tacgcaaaat ggcggataaa aacgcattaa gtctgtggat gcgagaacgt
     8821 agcgatcttt gggtgcagcc aaaagttgat ggcgttgcgg taaccctggt ttatcgggac
     8881 gggaaactga acaaagcaat cagtcgcggt aatggcctga aaggcgagga ctggacgcag
     8941 aaagttagct taatttccgc tgtgccgcaa accgttagcg ggcctttagc caacagtacg
     9001 cttcaggggg aaatatttct ccagcgcgag gggcatatcc aacaacaaat ggggggaata
     9061 aatgcccgcg caaaagttgc tggcctgatg atgcgccagg acgatagcga cacgctgaat
     9121 tctctgggcg tttttgtctg ggcatggccg gatggaccgc agttaatgac cgatcgttta
     9181 aaggagctgg ctaccgcagg ttttactcta acgcagaggt atacccgtgc ggttaaaaat
     9241 gctgatgagg ttgcgcgcgt acgcaatgag tggtggaaag cgaaattacc cttcgtcacc
     9301 gatggcgtgg ttgtgcgagg ggcgaaagaa cccgaatccc gccattggct accaggccag
     9361 gcagaatggc tggtggcctg gaaatatcaa cctgtagctc aggttgccaa agtgaaggcg
     9421 attcagtttg cggtgggtaa gagcggtaaa atatcggtgg ttgcgtcact cgcacctgtc
     9481 atgctggatg ataaaaaagt ccagcgggtg aatattggtt ccgtcaggcg ctggcaggag
     9541 tgggatattg cgcctggtga tcagatcctc gtcagccttg ccggtcaggg tattcctcgc
     9601 attgatgatg tggtctggcg cggtgcagaa cgtacaaaac cgacaccacc agaaaaccgc
     9661 tttaactcgt tgacctgcta ctttgcttct gatgtttgtc aggaacagtt catttcacgc
     9721 ttagtctggc tggggtcaaa acaggttctg gggctggatg gcattggtga ggccggctgg
     9781 cgcgcgctgc atcagactca tcgctttgag catatctttt cctggctttt attaacgcca
     9841 gagcaattac agaacacgcc ggggatcgcg aaaagtaaaa gtgcgcagct atggcatcgg
     9901 tttaatctgg ctcgtaagca gccttttact cgctgggtga tggcaatggg aataccgcta
     9961 acccgggcgg cgcttaatgc cagtgatgaa cggtcctggt cgcaactttt atttagcacg
    10021 gagcagttct ggcagcagct gccggggact ggatcggggc gcgccagaca ggttattgaa
    10081 tggaaggaaa atgcgcaaat caagaagctg ggcagttggc tggctgccca gcagatcaca
    10141 ggttttgaac cttagtgtcc ttcgtggctg tagtaaaaga ccggtaatcc cagcttaagg
    10201 cgtagcgcca gtaagcgggc aaaaaagccg aataccaggg tggagataat taccacatca
    10261 tggttagaaa catagtgttg cagcgcgatg tacagcacgg cggaggcaaa tgagacaccg
    10321 gcatacaact ctttctggaa taccagtggg atacgtttac agaacatatc gcgcaaaacg
    10381 ccgccaaaca cgccggtggt taccgccgcg acaacggcaa taattgggcc gtgccccata
    10441 tccagtgcaa cctgtgcgcc gatgatagaa aagaccacca gtccaagcgc atcgagcacc
    10501 agaaacactt tgcgcaggta aggcattaca ggagcaacga tagtggtgag tactgcggcg
    10561 gtagcgacga taatcacata ttccgggtgt ttgacccagc cgagcggata gtggcccagc
    10621 agaatatcgc gcactgaccc tccgccaatt gcggtggcgg tcgcaataat aattacgcca
    10681 aatgtatcca tgcggcgtcg cccggccgcc agagcccccg tcatggcttc ggcagtaatg
    10741 ccaaccaaat acaaaatgtg taacagcatg gttctctccc taaaaatctg tctggcaggg
    10801 tagcgatttg tgcgtattgt cacgattgag attttttaag gtgagaggaa attgataact
    10861 tgtagttatg tgaaatcgct attttctgta gcgtagatgc attcttctga cttcttgtct
    10921 gagaaaatgc ataagaaaaa ataggttaaa attgaggggt ggaaaagaaa ggtgcaatgt
    10981 ttagccattg tgttcaccgc acctggactt tggcttacgc atcgggtatc tcaatcaaca
    11041 aaaagagttt ttagtttctg ctatttcttc tctgtaattt ttacactgtt ttccaactgc
    11101 ttgatgctga cctgcggggt cggcaactcc tcaggcatgg ttccgccaag ttcctgaatg
    11161 gtttgcctca ccttgctacc cacatcaaag tgtgtggtat tagcttgctg ttttgaattc
    11221 acgccatccc gcttgagttt ttcttctgtt tgggtagctc gaaagagatt agccgccagt
    11281 tctgttgaac ccatatgatc caggatcttc tgactctttt tcagcccctt cagttggtgg
    11341 atggctttct gatctaatcc accatacagc ccctggtaac catgattctg aaatatggcg
    11401 aaatcagtag ctgtcgctac cgcagcttgc tgtgctgcct caaccaattg tttgttatgt
    11461 tctttcaact catttctcag aaacagacgc ttttcgtctt cacgaagttg cttaaatgct
    11521 tcatcgtcag caagctcctg ccgtcgggtc tggatagcaa aataagtctg ccctgccgca
    11581 atgaccggtt tcgcagggtc gccgttttgt actaccaggt aacaggcata gcgggagaga
    11641 tgaacatctt caagttctcg ttgagcccct gaacctaata caaccatttt ggtggtttca
    11701 acgaaatggt cagatgcagc ttgattgctg gcttcgcacg cttgagtcgc gcgggccagt
    11761 accttttgaa aattgcgcca gtcccgataa tccagtaacg gtgccaattc tctagctgac
    11821 caaaattctt gcccttcagt gccataatgc cgtatttctt caaaaggttg atgatgttcg
    11881 ttcattgccc actccatgtg atatcgattg caaagaaagt aaatctgtaa ctgtatttat
    11941 atacagttgt gagtatctca cgttctcagt gtgctgggca aaaaaacatc acaaaatctg
    12001 gagcaggcac gcaaaacaga attgaagttg cggggaagaa aaatgccgct ctgtttgagt
    12061 gggcggcatt tgtgaaggct gaaaagtaag aattggcgtt acgagttatt caatgttctg
    12121 aatctgctcg cgcatctgct caatcaacac tttcagctca atcgcggagt ttgtcacttc
    12181 ggcattgata gacttcgacg caagagtgtt cgactcgcgg ttgaattcct gcatcataaa
    12241 gtccagacga cgaccaacgg cttctttttt cttcagaatg ttgtaggtct ctttgacatg
    12301 cgcttccagg cggtccagtt cttcggcaac gtcaattcgt tgtgccagca gaaccagctc
    12361 ctgctccaga cggttgtttt ccaactgaac ctgagcatct tccagcttcg cgaccagacg
    12421 ctcacgctgc cattgcagga tttccggcat atgggcgcgg actttgacca cttcggcggt
    12481 gacgccttcc agacgctgct cgatcaatgc tttcagtgcc tgaccttcgg tttcgcgcgc
    12541 gacaataaag tcgtccagcg taccatccag cgccgcgaga atttcagcgg caatggcgtc
    12601 aagatcctgc tcctgggctg ccatcacgcc cggccagcgt agaatatcaa ccgggttgat
    12661 ttccccttcg tcactctgca tttttaccca gttcgcggca gttaccagct gtttagccag
    12721 tttttcgttg aggatcagct caccttgcgc gctaacatct ggctcatagc gcagggtaca
    12781 ttccacttta ccgcgcgtca ggcgagaacg aatacgctcg cgaacgacag gttcaaggct
    12841 acggaactgc tccggcagac gaaagtaagt ttccagataa cgctggttta ccgagcgcat
    12901 ttcccaggtt gcgctccccc attcaccctt gatttcacgc cgggcgtagg cggtcatact
    12961 gcggatcata gacgttcctg tttataaaag gagaggtgga aggattatag ccatcgatgc
    13021 cttgtcagga taggaataac cgccggaagt ccgtataatg cgcagccaca tttgtttcaa
    13081 gccggagatt tcaatatgcg tccagcaggc cgtagcaata atcaggtgcg tcccgttacc
    13141 ctgactcgta actatacaaa acatgcagaa ggctcggtgc tggtcgaatt tggcgatacc
    13201 aaagtgttgt gtaccgcctc tattgaagaa ggcgtgccgc gcttcctgaa aggtcagggc
    13261 cagggctgga tcaccgcaga gtacggcatg ctgccacgtt ctacccacac ccgtaacgct
    13321 cgtgaagcgg cgaaaggtaa gcagggtgga cgcacaatgg aaatccagcg tctgatcgcc
    13381 cgtgctcttc gcgcggcagt agatttgaaa gcgctgggtg agttcaccat tacgctggac
    13441 tgcgacgtgc ttcaggctga tggtggcacg cgtaccgcgt cgattacggg tgcctgcgtg
    13501 gcgctggcag atgcgctaca gaagctggtg gaaaacggca agctgaaaac caacccgatg
    13561 aaagggatgg tagccgcagt ttctgtcgga attgtgaacg gcgaagcggt ttgcgatctg
    13621 gaatacgttg aagactctgc cgcagagacc gacatgaacg tagtgatgac cgaagacggg
    13681 cgcatcattg aagtgcaggg gacggcagaa ggcgagccgt tcacccatga agagctactc
    13741 accttgttgg ctctggcccg agggggaatc gaatccattg tagcgacgca gaaggcggcg
    13801 ctggcaaact gatttttaag gcgactgatg agtcgccttt tttttgtctg tagaaaagta
    13861 agatgaggag cgaaggcatg aaaccatatc agcgccagtt tattgaattt gcgcttggca
    13921 agcaggtgtt aaagtttggc gagtttacgt tgaagtccgg gcgcaaaagc ccctatttct
    13981 tcaacgccgg gctgtttaat accgggcgcg atctggcact gttaggccgt ttttacgctg
    14041 aagcgttggt ggattccggc cttgagttcg atctgctgtt tggccctgct tacaaaggga
    14101 tcccgattgc caccactacc gccgtggcgc tggcggagca tcatgacctt gacctgccgt
    14161 actgctttaa ccgcaaagag gcaaaagacc acggtgaagg cggcaatctg gttggtagcg
    14221 cgttacaagg acgcgtaatg ctggtagatg atgtgatcac cgccggaacg gcgattcgcg
    14281 aatcgatgga gattattcag gctaatggcg cgacgcttgc tggcgtgttg atttcgcttg
    14341 atcgtcagga acgggggcgc ggcgagattt cggcgattca ggaagttgag cgtgattaca
    14401 actgcaaagt gatctctatc atcaccctga aagacctgat tgcttacctg gaagagaagc
    14461 cggaaatggc ggaacatctg gcggcggtta aggcctatcg cgaagagttt ggcgtttaaa
    14521 gaaactcgcc ggatgaaaag tcatccggcg tcatattact gcaactgtgc cgcaattagc
    14581 ggccagcggg cgtcaaaatc atccgtcggg cggtatttaa attcgctgcg gacaaaacgt
    14641 gacagcatac cttcacagaa ggctaggatc tggcttgcca gcagggtttc atcggtggtg
    14701 taaccttcac cctcacgcat tctcttttca cgcaatacct gacgcagctg cgcttcaata
    14761 cgctcgaaca gctggttgat gcgcccttgc aggcgatcct gttcaaacat tagcgcatga
    14821 ccagtgagga tgcgggtcag gccaggatta cgctcaccaa aaccgagaag cagcaacaca
    14881 atcagacgca ggcgcgctgt ggtgtctttc tcatctttca gaatcagatt gatgcgagta
    14941 atcaggctat cttcgataaa ctcaatcagg ctatcgaaca tgcgggtctt actggggaag
    15001 tggcgataca gtgccgcttc ggaaacgccg acagaggcgg ccagttttgc cgtcgtgata
    15061 cgttggcttc catcgctgga ttccagcatc agcgccagag actgaagtat ttcctcgcga
    15121 cggttccttt tcgcagtttg tttttctgcc atgttacaaa atacccctga aaataagcac
    15181 ttgccaggcg gcacccacgc tatgaccgca aacgaaatgt ttacggctat gttatgacgt
    15241 tattcggatg cgtatgtgtt actgacgacc agagtgacca aagccgcctt caccgcggtc
    15301 ggtggcgtcg aaatcttcca ccagattaaa ttcagcctgt actaccggaa caaaaatcat
    15361 ctgggcgata cgttcgccag gttgaatggt gaagctgtcc tgaccacggt tccacacgga
    15421 aatcatcaac tggccctgat agtcggaatc gatcaatcct accaggttac caagcacgat
    15481 accgtgctta tgtcccaatc cggagcgcgg cagcatcatt gccgccagtg aaggatcggc
    15541 aatatgaatc gccagcccgg tcggaaccag cgtagtgtca cccggagcca gttctacggc
    15601 gtcgtcgaga caggcacgca ggtcaagtcc ggcagagcca gaggtggcat aagtcgggag
    15661 cggaaattcc ttcccaacgc gcgggtccag aatcttaacg tcgatttttt tcatcataac
    15721 gggtcacgat ctcgtcgagt aataattggc caaggagctc tttgcgctca agcggtaaga
    15781 ctttatctcc gtcctgccag aaaaggtgta atgcgttgtt gtcgctgtta aatccttgag
    15841 ttggctggga aacatcgttc gcgcagatca gatcaaggtt tttacggata cgtttttgcc
    15901 gggcgtattc ttccacatta tttgtttcgg cggcaaatcc aacgacgtag ggtcgatggt
    15961 cttttagtgc ggcaacgcct gcgacgatat cggggttttt aaccattttt attgttaatt
    16021 catcaccctg cgtggcctgc tttttgattt tctctggggc cacggtagct gcgcgataat
    16081 ccgccacggc ggcgcagccg ataaaaatat tttgctgctg tacagaagca ttcacggcgg
    16141 cttccatttc cagcgcggtc atcacatcaa cacgtttaac aaacggtggc gtcggtagtg
    16201 aaaccggacc tgataccagc gtgacgttcg cgccacgacg ggcagcggcg gcggcgatag
    16261 caaaacccat cttgccggag ctgtgattag agatataacg cacgggatcg agcggttcac
    16321 gcgtcgggcc ggcggtaatc ataatgttca gatgtttcag gtcgttgacg ggcgaaaaat
    16381 gcgctaccgc catatccaca atggttaacg gatcgagcat tcgcccagga ccgatatcac
    16441 cacaagcctg actgccactg tctggccccc agatgagcaa accacgggaa gcaagcacct
    16501 ctaaattatg ctgcgtggca gcggcacggt acatctgctg gttcatggcg gggagcacgg
    16561 ctacaggcgc aggtgtagcc agacaaatcg tcgataccag gtcattcgcc attccggcag
    16621 caacacgggc aatcaaatct gccgtggcag gggcgagaat cactaaatca gcccatttac
    16681 ccagctcaat atggcccata gcggcttcgg ctgccgggtc cagcaaactg tcggaaacgg
    16741 gataaccaga aaccgcctgc aagctaagtg gggtgataaa ggcttttgcc gcttcggtca
    16801 tggctacgcg gacgtcggcc ccgcgatcgc gcaaacgacg caccagttca ggggttttat
    16861 aggcagcaat accgccgcta acgccgagaa cgattttttt accggccagg ctcatgatga
    16921 tttttcctgt tgggtgacac cagaagttgg cgattttatc acaatacttt tgttgtcgtg
    16981 ccttcacctg agattcactt tgcgaggcgc tttccaggat tgaaaactgg ccgtcgattt
    17041 aacggaacgg ctatgacagg atgcgagcac cacaaaggag gtgaaggtga aaaacaatgc
    17101 acagctgttg atgccgcgcg aaaaaatgct gaagtttggt attagcgcct taactgatgt
    17161 cgagctgctg gcgctatttc tgcgtaccgg aacgcgcggt aaagatgtat taaccctggc
    17221 aaaagagatg ctggagaatt tcggctctct ttatggcttg ttaacctctg aatatgaaca
    17281 atttagtggt gttcatggaa ttggcgtagc gaaatttgcc cagttaaagg ggattgctga
    17341 actggcgcga cgttactaca acgtgcggat gcgtgaagaa agccctttac tcagcccgga
    17401 gatgacgcgg gaatttttac aaagccagct cacgggtgag gagcgggaga tctttatggt
    17461 gatctttctc gactcccaac accgggttat aacgcatagc cgtctttttt ccggcacgct
    17521 aaaccatgtt gaagtccatc ctcgggaaat tatccgcgaa gcgataaaaa taaacgcctc
    17581 ggcgctgatc cttgcacata atcacccttc gggttgtgct gaacccagta aagcggataa
    17641 actcattact gaacggataa taaagagttg tcagttcatg gatttacgcg tgctcgacca
    17701 tatcgtgatt gggcgtggag agtatgtttc ttttgccgaa cgcggctgga tttaacccgc
    17761 tatgcgcgat ccttcgggat ctttgtctgt tcgggacttg agcacatcgc tgagtcagcg
    17821 tatactacgc cacctttgag aatctcgggt ttggcatttg ggcctggcaa tcgagagttc
    17881 acatagaact gcgatgaccg ggctgtaaag cctgacgagg cgccaatacc ccatacgaag
    17941 ctcgagctaa tttgattttt ggagaataga catgtcccga gtctgccaag ttactggcaa
    18001 gcgtccggtg accggtaaca accgttccca cgcactgaac gcgactaaac gccgtttcct
    18061 gccgaacctg cactctcacc gtttctgggt tgagagcgag aagcgttttg tcaccctgcg
    18121 cgtatctgct aaaggtatgc gtgtaatcga taaaaaaggc atcgatacag ttctggctga
    18181 actgcgtgcc cgtggcgaaa agtactaagt acttagagga aataaatcat ggctaaaggt
    18241 attcgtgaga aaatcaagct ggtttcttct gctggtactg gtcacttcta taccactacg
    18301 aagaacaaac gtactaagcc ggaaaaactg gaactgaaaa aattcgatcc agttgttcgc
    18361 cagcacgtga tctacaaaga agcgaaaatc aaataattct cgctttgatg taacaaaaaa
    18421 cctcgctccg gcggggtttt ttgttatctg cttgccccca tattgactgc atctgttcat
    18481 tcctggagat gctatgcctg aattacccga agttgaaacc agccgccgcg gcatagaacc
    18541 gcatctcgtt ggtgcaacca ttcttcatgc agtggtgcgc aacggacgct tgcgctggcc
    18601 ggtttcagaa gagatctacc gtttaagcga ccaaccagtg cttagcgtgc agcggcgggc
    18661 taaatatctg ctgctggagc tgcctgaggg ctggattatc attcatttag ggatgtctgg
    18721 cagcctgcgc atccttccag aagaacttcc ccctgaaaag catgaccatg tggatttggt
    18781 gatgagcaac ggcaaagtgc tgcgctacac cgatccgcgc cgctttggtg cctggctgtg
    18841 gaccaaagag ctggaagggc ataatgtgct ggcccatctt ggaccggagc cgcttagcga
    18901 cgatttcaat ggtgagtatc tgcatcagaa gtgcgcgaag aaaaaaacgg cgattaaacc
    18961 gtggctgatg gataacaagc tggtggtagg ggtagggaat atctatgcca gcgaatcact
    19021 gtttgcggcg gggatccatc cggatcggct ggcgtcatca ctgtcgctgg cagagtgtga
    19081 attgttagct cgggtgatta aagcggtgtt gctgcgttcg attgagcagg gtggtacaac
    19141 gctgaaagat tttctgcaaa gtgatggtaa accgggctat ttcgctcagg aattgcaggt
    19201 ttacgggcga aaaggtgagc cgtgtcgggt gtgcggtacg ccgattgtgg cgactaaaca
    19261 tgcgcagcgg gcaacgtttt attgtcggca gtgccagaag taattcatgc gcgccggatg
    19321 gcataccatc cggcataaac gctacgctaa cttcgccatc agcgcctgat ggacattctc
    19381 cggcaggaaa tgggtgacat cgccctgatg gcgcgccacc tctttcacca acgatgaaga
    19441 gataaacgac cactctttcg acggcatcag aaacacactt tccagttccg gcattaagtg
    19501 gcgattcata tgcgccagct gcatttcata ttcaaaatct gccaccgcac gcaggccacg
    19561 aatcagcacc gtagcgtgtt gattacgggc gaagttcgcc atcaaatcac taaacccgac
    19621 cacttccacg ttccccagat gcgcggttgc ctgctgtgcc agtgccacac gctcttccag
    19681 ggtaaacatc ggttttttac tggggctggc ggcaatcgcc agaataacgt gatcgaacat
    19741 ctgcgtggcg cgcgtcacga tatcgatatg accattggta atgggatcga aagtacccgg
    19801 ataaatcgcc cgtttttgca taacaacctc aatgcgtttt cggtggcagg taaggttcca
    19861 gcagttgaag cagacgctgt agcgcgccct ggttttgata cagtacttca acggcatgac
    19921 ggccatagaa actacggtaa tcggcgtcgg tgagtaaaga ggaaacctct tttgcaagcg
    19981 tagtggcatc ggtaacggta atcagcccgc ttgcctgctc cagccgcgcg caaatgtctt
    20041 taaagttaaa agtatgcggc cccatcaata ccggaattgc gtgtgcggca gcttccagcg
    20101 gattatgccc accacgttca accagtgaac cgccaacaaa ggcgagatcg gcaatgccgt
    20161 acagtaacat caactcgccc atcgtatcgc caaccacaac ctgcgtgctg gtggagggga
    20221 cttcccctga agagcgtgtg atatagctta gtccagcctg gcggacaagg ttaatcgcat
    20281 ccgggaagcg ttctggatga cggggtacca ggatgagcaa taaattcggg aattgctgta
    20341 ataaagcctg atgtgcggca atgaccacgc tctcttcacc ttcgtgagtg ctggtggcaa
    20401 tccataccgg gcggtgtggt gcccactggc ggcgcagcgt cacggcttta gcagccaact
    20461 gcggcgttac agaaatatcg aatttcaggc taccggtaac ggttacctgg ttatttttcg
    20521 cgcccagcgc cacaaaacgt gcaccatctt cttcattttg ggcggcaatc agcgtaatac
    20581 gacgcagcaa gcgacggacg aatttaccca gtttggcata acctgcggcc gagcgggcag
    20641 agagtcgcgc gttagcgatc accagcggaa ttttacgttt atgtagcgcc gcaatcaggt
    20701 taggccatag ttcggtttcc ataatcaaca ccagtttagg gtcgacttta ttcaggaaac
    20761 ggttgagtgc atcgggcaga tcatacggca gataaacgtg ctgaacatcc ttcccgaaag
    20821 ccgattgtac gcgctccgaa ccggttggcg tcatggttgt tacggtaata ggtaaatcag
    20881 gataacgatg acgcagcgcg cgcaccaacg ggatcgccgc cagagtttca ccgacggaga
    20941 cggagtgcag cataatgccg cctggtttta gcggatggcg gtaaaaaccg taacgttcac
    21001 cccagcgttt tcgataggcc ggagccttac gtccgcgcac ccagagccgt atccagatca
    21061 gcggctgaat aaggtagaga agggcggtgt aaagcaattc gagcatagta aatagctgac
    21121 ttatggatgt gctggggatt ctatgtattt agctgtggct ttaccattac ttttcccgtt
    21181 tttgacttaa atagcaattt acccgccaat tcttgctgaa catctatact ttttttgtat
    21241 ttttatgcga ttcattgatt tctgctgtac gtgcaggaag gaatcgcaga gcaacagtga
    21301 aagaatatgg ttgctaatga ttttatttga ttgatttctt tattgaaaat caacaatgtg
    21361 cttaaaaatc attcgggtcg gattgccgcg aaagaagtga tacactagcg cgtcaatatc
    21421 agtgcggtat cagtattcag gtagctgttg agcctggggc ggtagcgtgc tttttctgct
    21481 tcaccttaat cggataatct caacaaaaag agttacttgt ggataagcca tttcgaagaa
    21541 ttttgctcat taagatgcgt tttcatgggg atatgttatt aactactccc gtcattagtt
    21601 cgctgaaaaa aaattaccct gacgcaaaaa tcgatgtgct gctttatcag gacaccatcc
    21661 cgatcctgtc tgaaaatcca gagattaacg cgctctacgg cataaaaaat aaaaaagcaa
    21721 aagcctcaga aaaaattgcc aacttttttc atctcatcaa ggtattacgt gccaataagt
    21781 atgaccttat cgtcaatctt accgatcaat ggatggttgc tatactggtt cgcttattaa
    21841 atgcccgtgt gaaaatttcc caggattatc atcatcggca gtctgctttt tggcgtaaaa
    21901 gtttcaccca tttggtgccg ttgcagggtg gaaatgtggt ggaaagtaac ttatccgtgc
    21961 tgacaccatt gggacttgat tcgttggtga agcagacaac catgagttac ccgcctgcaa
    22021 gctggaaacg tatgcgtcgc gaacttgatc acgctggtgt tggacaaaat tatgtggtta
    22081 tccaacctac ggcgcggcaa atcttcaaat gctgggacaa cgccaagttt tccgctgtga
    22141 ttgatgcctt acatgctcgt ggttatgaag tcgttctgac gtccggccca gataaagacg
    22201 atctggcctg cgtcaatgaa attgcgcaag gatgccagac gccaccagta acggcgctgg
    22261 ctggaaaggt gaccttcccg gaacttggtg cgttaatcga tcatgcgcag ctgtttattg
    22321 gcgttgattc cgcaccggcg catattgccg ctgcagttaa tacgccgctg atatcgctgt
    22381 ttggtgcgac agaccatatt ttctggcgtc cttggtcaaa taacatgatt caattctggg
    22441 cgggagatta ccgggaaatg ccaacgcgcg atcagcgtga ccgaaatgag atgtatcttt
    22501 cggttattcc ggcggcagat gtcattgctg ctgtcgataa attactgccc tcctccacga
    22561 caggtacgtc gttatgatcg ttgctttttg tttatataaa tattttccct ttggcggttt
    22621 gcagcgcgat tttatgcgta ttgctcagac agtcgccgcc cgaggtcatc atgttcgggt
    22681 ttatacccag tcgtgggaag gcgaatgccc tgatgtattt gaactgatca aagtgccggt
    22741 taaatcgcat accaatcacg ggcgcaatgc ggagtatttt gcctgggtgc aaaaacattt
    22801 acgcgaacat cccgtcgata aagtcgttgg attcaacaaa atgccggggc tggacgttta
    22861 ttatgccgct gatgtttgtt atgccgagaa agtcgcgcag gaaaaaggct ttttctatca
    22921 cctgacgtca cgttatcgcc attatgccgc ctttgagcgg gcaaccttcg aacagggcaa
    22981 gccgacacag ctgctgatgc tgacagataa gcaaatcgcc gatttccaga aacattatca
    23041 gactgaagcg gagcgttttc atattctacc accggggatt tatcctgatc gtaaatatag
    23101 ccagcagcca gctaatagcc gtgaaatctt ccgtaagaag aatggaataa ccgaacaaca
    23161 atatttattg ttgcaggtcg gttcagactt cacgcgtaaa ggtgtcgatc gttccattga
    23221 agcacttgct tcgttaccgg attcgctgcg ccacaacaca ttgctatatg ttgttggaca
    23281 ggataaaccg cgaaaatttg aggcgctggc agaaaaacgc ggcgtgcgca gtaatgttca
    23341 cttcttctcg gggcgcaacg atgtctcgga attaatggcg gcggcggatt tattactgca
    23401 tcctgcctac caggaagcag cgggaattgt gctactggaa gcgattactg caggattacc
    23461 ggtactaaca acagccgttt gtggctatgc gcattatatt gtcgacgcca attgcggcga
    23521 ggctattgct gagccattcc gccaggaaac attgaatgag attttacgca aagcgttaac
    23581 gcaatcttca ttgcgccagg cctgggcgga aaatgcgcga cattatgctg atacacaaga
    23641 tttatacagt ctgccagaga aagcggcgga tatcataacg ggtggtctgg atggttgaac
    23701 ttaaagagcc gtttgccacg ttatggcgcg gtaaagatcc ttttgaggaa gttaaaacct
    23761 tgcagggtga ggtttttcgt gaactggaaa ctcgccgcac tctgcgattt gaaatggcgg
    23821 gcaaaagcta ttttctcaaa tggcatcgcg gcacgaccct gaaagagata atcaaaaatt
    23881 tactctcatt gcggatgcca gtattaggcg cagaccgcga atggaatgcg attcatcgac
    23941 tgcgggatgt cggcgttgat actatgtatg gggtggcatt tggcgaaaaa ggcatgaatc
    24001 cgctgaccag aacttcattt attattaccg aagatctgac accaaccata agtcttgaag
    24061 attactgtgc tgactgggcg actaaccctc cagatgttcg cgtaaagcgt atgcttatta
    24121 agcgtgtcgc gacgatggtg cacgatatgc atgctgcggg cattaaccac cgtgactgtt
    24181 atatctgtca tttcctgctg cacttgcctt tttccggtaa ggaagaggag ttaaaaattt
    24241 cggtaattga cctgcaccgg gcgcagcttc gcacgcgcgt tccacgtcgt tggcgggata
    24301 aagatcttat tgggctttat ttttcttcga tgaatatcgg cctgactcag cgggatatct
    24361 ggcggtttat gaaagtgtat tttgccgccc cgcttaaaga cattctcaag caggaacaag
    24421 gactgctgtc gcaagcagaa gcaaaagcca caaaaatcag ggaaagaacg attcgaaaat
    24481 cgttgtaacc cgtaacttat ttttgccaaa attttggata cagaataaat atgtctcaac
    24541 tcaatgatag tgacatcatc ctttttgagt ataattttca ttatcaaaat ataagatcta
    24601 aaaatactct tgatatagca tttggtattg acagaaattt tctttttgga tgtggtgtag
    24661 ccatcgcatc tattctatta aacaatagag aaatctcttg tgaatttcat gttttcacag
    24721 attatatcag tgataaagac aaattatatt tttctgattt agcaaaacaa tataattcaa
    24781 gaattaatat ttatgttatc aattgtgata agctgaagtc attaccaagc acgaaaaact
    24841 ggacttacgc aacatatttt cgatttataa ttgcagatta tttctatcat aaacatgaaa
    24901 aaatactata tcttgatgca gatattgctt gcaagggtag tattaaagaa ctcttagatt
    24961 atcaattttc tactaatgaa attgccgcag ttgtagctga aagagatgtt gaatggtggc
    25021 aaaatcgagc ctcggtatta actacaccac agttagcttc tggatatttc aatgctggtt
    25081 ttttactgat aaatattgat gagtggaatc taaataacat ttcgtcaaaa gctattgaaa
    25141 tgttgcgtga cccagattgg gtaagtaaaa tcacccacct tgatcaagat gtactgaatg
    25201 tattattgaa tggtaaagtg aagtttattt cggagaaata taatacccga tatagtatta
    25261 actatgaatt aaaagacaaa gttgataatc cagtcaatga tgacaccgtg tttatacact
    25321 atgtgggacc tacaaaacct tggcatgagt gggctgacta tccggtgtca cgtagttttt
    25381 tgatcgccaa agcagcttct ccgtggagta aagaagattt acttaaacct gtaaatagca
    25441 atcagtatcg gtattgtgca aaacataa
//
LOCUS       DAZFAB010000066        25238 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZFAB010000066 DAZFAB010000000
VERSION     DAZFAB010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25238
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.066"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            293..1117
                     /gene="bax"
                     /locus_tag="ACHJWA_003009"
     CDS             293..1117
                     /gene="bax"
                     /locus_tag="ACHJWA_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFO4309560.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTSRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            complement(1225..2403)
                     /gene="xylR"
                     /locus_tag="ACHJWA_003010"
     CDS             complement(1225..2403)
                     /gene="xylR"
                     /locus_tag="ACHJWA_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HFO4309561.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(2481..3662)
                     /gene="xylH"
                     /locus_tag="ACHJWA_003011"
     CDS             complement(2481..3662)
                     /gene="xylH"
                     /locus_tag="ACHJWA_003011"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFO4309562.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            complement(3640..5181)
                     /gene="xylG"
                     /locus_tag="ACHJWA_003012"
     CDS             complement(3640..5181)
                     /gene="xylG"
                     /locus_tag="ACHJWA_003012"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFO4309563.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            complement(5259..6251)
                     /gene="xylF"
                     /locus_tag="ACHJWA_003013"
     CDS             complement(5259..6251)
                     /gene="xylF"
                     /locus_tag="ACHJWA_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO4309564.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLQPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            6617..7939
                     /gene="xylA"
                     /locus_tag="ACHJWA_003014"
     CDS             6617..7939
                     /gene="xylA"
                     /locus_tag="ACHJWA_003014"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFO4309565.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAACMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            8011..9465
                     /gene="xylB"
                     /locus_tag="ACHJWA_003015"
     CDS             8011..9465
                     /gene="xylB"
                     /locus_tag="ACHJWA_003015"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFO4309566.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECALLEARVPLSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLSNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            9634..9975
                     /gene="yiaB"
                     /locus_tag="ACHJWA_003016"
     CDS             9634..9975
                     /gene="yiaB"
                     /locus_tag="ACHJWA_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFO4309567.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            10021..10458
                     /gene="yiaA"
                     /locus_tag="ACHJWA_003017"
     CDS             10021..10458
                     /gene="yiaA"
                     /locus_tag="ACHJWA_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFO4309568.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQRNIRDAGINPPKETQVTQEEYSE"
     gene            complement(10500..11495)
                     /gene="wecH"
                     /locus_tag="ACHJWA_003018"
     CDS             complement(10500..11495)
                     /gene="wecH"
                     /locus_tag="ACHJWA_003018"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFO4309569.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLFFYSAIALLYIALFTSINV
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRALGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYPYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            11670..11969
                     /gene="ysaB"
                     /locus_tag="ACHJWA_003019"
     CDS             11670..11969
                     /gene="ysaB"
                     /locus_tag="ACHJWA_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFO4309570.1"
                     /translation="MMMNSFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            12064..12975
                     /gene="glyQ"
                     /locus_tag="ACHJWA_003020"
     CDS             12064..12975
                     /gene="glyQ"
                     /locus_tag="ACHJWA_003020"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFO4309571.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLARENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            12985..15054
                     /gene="glyS"
                     /locus_tag="ACHJWA_003021"
     CDS             12985..15054
                     /gene="glyS"
                     /locus_tag="ACHJWA_003021"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFO4309572.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPSEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRINRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            15377..15529
                     /gene="hokA"
                     /locus_tag="ACHJWA_003022"
     CDS             15377..15529
                     /gene="hokA"
                     /locus_tag="ACHJWA_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFO4309573.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQGSYELAAFL
                     ACNLKE"
     gene            complement(15506..15577)
                     /locus_tag="ACHJWA_003023"
     CDS             complement(15506..15577)
                     /locus_tag="ACHJWA_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4309574.1"
                     /translation="MRNAVRKAGITFRRRLLLFQIAG"
     gene            complement(15717..15929)
                     /gene="cspA"
                     /locus_tag="ACHJWA_003024"
     CDS             complement(15717..15929)
                     /gene="cspA"
                     /locus_tag="ACHJWA_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFO4309575.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(16210..16500)
                     /gene="yiaG"
                     /locus_tag="ACHJWA_003025"
     CDS             complement(16210..16500)
                     /gene="yiaG"
                     /locus_tag="ACHJWA_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFO4309576.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            16934..17644
                     /gene="yiaF"
                     /locus_tag="ACHJWA_003026"
     CDS             16934..17644
                     /gene="yiaF"
                     /locus_tag="ACHJWA_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFO4309577.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(17694..18668)
                     /gene="ghrB"
                     /locus_tag="ACHJWA_003027"
     CDS             complement(17694..18668)
                     /gene="ghrB"
                     /locus_tag="ACHJWA_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFO4309578.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(18772..19431)
                     /gene="yiaD"
                     /locus_tag="ACHJWA_003028"
     CDS             complement(18772..19431)
                     /gene="yiaD"
                     /locus_tag="ACHJWA_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFO4309579.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            19584..21917
                     /gene="bisC"
                     /locus_tag="ACHJWA_003029"
     CDS             19584..21917
                     /gene="bisC"
                     /locus_tag="ACHJWA_003029"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFO4309580.1"
                     /translation="MTNSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVNAEKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRAAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMDRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFTEFWQANQL
                     IEMPENPDSERFIRFADFCRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLVP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGIQD
                     GDTVRLWNARGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(21886..22326)
                     /gene="yiaC"
                     /locus_tag="ACHJWA_003030"
     CDS             complement(21886..22326)
                     /gene="yiaC"
                     /locus_tag="ACHJWA_003030"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO4309581.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKVNYWRECIPLVRDAY
                     LANAKNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYQAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(22323..22886)
                     /gene="tag"
                     /locus_tag="ACHJWA_003031"
     CDS             complement(22323..22886)
                     /gene="tag"
                     /locus_tag="ACHJWA_003031"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFO4309582.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDRKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            23044..23742
                     /gene="yhjY"
                     /locus_tag="ACHJWA_003032"
     CDS             23044..23742
                     /gene="yhjY"
                     /locus_tag="ACHJWA_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO4309583.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALSQAENTTN
                     NSDYLYTMGVSARF"
     gene            23971..25173
                     /gene="yhjX"
                     /locus_tag="ACHJWA_003033"
     CDS             23971..25173
                     /gene="yhjX"
                     /locus_tag="ACHJWA_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4309584.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTIASGILLGLGFFLTAHSDNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVKFYVIFALLILSLALSTTIRQPEQ
                     KVLREAHI"
BASE COUNT         6276 a         6098 c         6401 g         6463 t
ORIGIN      
        1 tcagacagtt tgccagcgat aagccagaca aaactcatcc ctgagcggta agtccacagg
       61 atgagaagca agggtgagcg atcgcgcgca aaaaacggct gaattttgcg ataaacccca
      121 cattttctgc gatttagcgc caatctgaat cgttaactcg tgacatagtt tcagatttgg
      181 actattcctt ggggtgctct tgacagctat ttttgatatg atttgagatt caactctcaa
      241 atttgtgaaa aatataaggt gttggaatga ttaaatccga ccaggagacc tgatgatttt
      301 gactcccata cgacgatatg gggcgatgat tcttatgtta ctcactctgg tgttttcgag
      361 tgaggtgtta gcgaagacgc acacaacaac agcgagtcaa aagtcccact taactaaagc
      421 tagtaataaa caggtaagca gtaaacaaga gtattctcgc aatagtgcaa agagtagttc
      481 acttcctgat ttgcgaaaat acccttccgg aacatcaagg aaaaaggcgt ttctccggac
      541 cgtaatgcct tacattacca gccaaaatgc agccattact gcagaacgta actggctgat
      601 ttcaaaacag tatcagggcc aatggtcacc tgctgagcgt gcgcgtctga aagacatcgc
      661 caaacgctac aaggtgaagt ggtctggtaa tacgcgaaaa atcccgtgga ataccttgct
      721 tgaacgcgta gacattatcc ctaccagtat ggtggcgacg atggctgcag cagaaagcgg
      781 ttggggaacg tcgaagctgg cgcgcaacaa caacaatctt ttcggcatga aatgcatgaa
      841 aggacgttgt accaatgcgc caggtaaagt gaaagggtac tcacagttta gttctgtcaa
      901 agaatcggtg agcgcctatg tcactaacct caatacgcat ccggcttact cttcgttccg
      961 taaatcgcgt gcgcagctgc gtaaagctga tcaggaagtg actgccacag cgatgattca
     1021 caagctgaag ggctactcga ccaaagggaa gagttataac aactacctgt tcgcaatgta
     1081 ccaggataac caacggttaa tcgcggcgca tatgtaattg tatttcctcg agccttatcc
     1141 gacttgtcgg tcggataagg cttttgatcg catcaggtgg ttgtgctgag ttacctgatg
     1201 cgacgccgac aattctcatc atcgctacaa catgacctcg ctatttacat cgcgatactc
     1261 ttttggcgtc gtgtcatatg cttttttaaa aacagagtag aaatattgca gcgatggata
     1321 accgcacatt tgcgatatct cattgatcga caaggtggtt gaaatcagca gactgcgcgc
     1381 tttctccagc ttctcagcat gaatcatggc atggatggtt tcacccacct cttctttaaa
     1441 acgcttctca agattggagc gcgagatccc gaccgcatcg agtacctgat ccactttaat
     1501 ccctttacag gcgtgattac gaatgtaatg catggcctga ataacggcgg gatcggtcag
     1561 cgaacggtaa tctgttgagc gccgttcaat gacgcgaact ggagggacca aaatccgctg
     1621 tagcggcatt tcttctttat ctaataatcg atgcaacagt tttgccgcct gatagcccat
     1681 ttgccgcgcg ccctgagcga ccgaagaaag ggcgacacgc gacagatagc gggtcagttc
     1741 ttcgttatcg atgccaatca cgcataattt ttccggtacg ggaatgtgta gatgttcaca
     1801 tacttgcaga atatgccgcg cccgggcgtc agtaacggca ataatcccgg tttgcggtgg
     1861 cagtgtttgt agccagtctg ccagccgatt ttgcgcgtgt tgccagttct ctggtgcggt
     1921 ttctaacccc tgataaacca ctccgcgata cttttcttcg gcgacaagct gacgaaatgc
     1981 atattcgcgt tcagtggccc aacgtttgcc gcttgattcc ggaagaccat aaaaagcaaa
     2041 gcggttaacg cctttctctt ttaaatgcaa aaatgcgctt tcaaccagcg catagttatc
     2101 ggtggcaatg taatgaacgg gtgggtaact ttctgcaagg tgatacgagc cgccaacccc
     2161 aacaatgggg acgtcgacat cagccagcgc ttgctcgatc tgtttgtcgt cgaagtcggc
     2221 aatgacgcca tctcctaacc agtccttgat tttatcaatg cgggcgcgga aatcttcttc
     2281 aatgaaaata tcccattccg attgtgacgc ctgtaaatat tcccctacgc cttctactac
     2341 ctgccggtca taggctttat tggcattgaa cagtaatgtg atgcggtgac gtttagtaaa
     2401 catggttctt ttcctgctga atcatgcaaa aactcaaaac cggtaatacg ttaccggctt
     2461 tgagaaaatt tttatcaaaa tcaagaacgg cgtttggttg cggagtccat ccatactgcc
     2521 agcaacagaa tcgcaccttt aacgatatac tgccagaagg tcggtacatc catcatactc
     2581 ataccgttat ccagtgaagc catgatgaat gcccccatta ccgctccagc cacgctcccg
     2641 acgccgcctg ccagactggt gccgccaatc acgcatgctg caattgcgtc cagctcggcg
     2701 atattccccg cagaaggtga accagcgcca agtcgagaac tgaggattaa tccggcgatg
     2761 gctaccatta atccgttaat agcgaacacg gcaagtttgg tgcgttcaac gttaattccg
     2821 gagagacgtg ctgcttccag attgccgccg atggcataaa tgcgtcgtcc aaatgctgtc
     2881 cgcgttgcca taaacattcc gccgagtaac agcaacgtca gcagcagaac aggagtagga
     2941 acgccacggt aatcattcaa cagccagatt gcgcctaata cgatgatagc ggttaaagcc
     3001 tggcgaccga ctactgcggt agaggctgga gactgcaaac ccaaagcctg acggcgcatt
     3061 cttccgcgcc attgccagcc aacaaaagcc attaagccaa gcgcgccaat gatgaagccg
     3121 gtgctggcgg ggagatagct ttgcccaatt tgtgacatcg cggcgctggt gggggataca
     3181 gtcgtgccgt tggtgatgcc aatgagtatg ccgcgaaatg ccaacatgcc cgcgagagtg
     3241 acaataaatg aagggacttt gcggtacgcg acccaccatc cgttccaggc accgagaagc
     3301 agtcccagaa ccaacgtcac aatgatggta agtggcaaag gccagcctag ccagacgtca
     3361 caaatcgccg cgacgccacc taatagcccc atcattgagc cgacggaaag gtcgatttca
     3421 gcagaaatta tgacgaacac cattcctacc gcgaggatgc cggtaatcgc ggtctggcgt
     3481 aacaggttgg agacgttacg ggcgcttaag taggcaccat cggtggtcca ggtaaagaac
     3541 agcatgattg cgatgatagc tgcaatcatc acgaagacct gcaaattcag tgatttcagc
     3601 ccggagaagc caccggatgt cggtacggcc aatttcactt cagacggatt gcttttcgac
     3661 atgatgttcg ctcctcaatg cggcttccat cacctgctcc tgagtcaggt tatgatttat
     3721 caggttggct tttagtttcc cttcatgcat tactagtaca cgatcgctaa ggccgagcac
     3781 ttcaggtaat tcagaagaga tgacaataac ggcaataccc tgctggacga gttggttaat
     3841 taatttgtag atctcgtatt tcgcgccaat atcgataccc ctggtgggtt catcaagaat
     3901 gagaatgcgc gggttaagta acagacagcg agcgaggatc gctttttgct gattgccgcc
     3961 gctcaaacgt ccaatagcaa ggtcggggga cgacgtttta actttgagtt gctggattga
     4021 ttccagaata catttttgct ctgccgcgtc atcaagctgg ctaatgccac cggtaaattt
     4081 attgagtgcg gcgagggtaa tatttttacc aaccgccatt accggaacga tgccgtcgcg
     4141 ctttctgtct tcggggacca tcgcaatccc ctgggcgatg gcttgctgac agttacgaat
     4201 atctacctgt ttgccatcaa tataaatttt tccttcccat tgtccgggcc acacaccaaa
     4261 caggcactga atggtctcgg tacgtccggc accaacgagt ccggcaatac ccagtatttc
     4321 gccacgtttc agggaaaacg agacatcatt aactcgttta atatgacgat taaccggatg
     4381 ccatgccgtc agatgttcaa tacgtaatat ttcatctccg gtggtatgtg gttcattagg
     4441 gtaaagcgcg gttaactctc gcccgaccat catggtgata atatcgtctt cactcattcc
     4501 ggcagcatca cgcgtaccaa tgtgctgtcc gtcgcgaata acgcaaatcg tatcggaaat
     4561 cgctttgact tcgttgagtt tgtgcgaaat ataaatacag gcgataccgt gctgttgtag
     4621 atcgcgaata atatccagta aaaccgacgt ttcctgctca gttaatgagg ctgtcggttc
     4681 atcgagaatt aacaagcgca cctgtttatt aagtgccttg gcaatttcaa ccagttgttg
     4741 ttgcccaagc cctaaatcgc caacgcgggt atcaggtgaa atggataaac tgacctgtgc
     4801 gagcagcttc tgacagcgta gcgtcatcag gtcataatcc ataatgccat tgtgggttat
     4861 ttcgttaccc aggaagatat tttccagcac ggtcaattct ttcaccaggg ccaattcctg
     4921 gtgaatgatg gcgatacctt tgcgttcggt atcgcggatg tgactcgcct gaatctcttc
     4981 tcccgcaaaa ataatttcgc cttcgtagga gccatgggga taaataccac acagcacttt
     5041 catcagcgtt gatttaccag acccattttc cccacagagt gagacgattt cgccagcatt
     5101 caaccgcaag ctgacgttat caatcgcctt cacactgccg aaggttttgg taatgttctt
     5161 catttcaagt agataaggca tagcgactcc acctaagcca attcattcac gcggcatgga
     5221 gagaaatcac gcccccgctc cgcgctgggg cgtaacgctt acagctcgct ctctttgtgg
     5281 aatccgtctt taattaccgt atctttgatg ttgtttttat tcacatcgat cggtgtcagg
     5341 aggcgggagg ggacatcttt caggccatta tttagtgaag tatctgcttt tggctcctga
     5401 ccattgccca attcaacggc aatttctgcg gcagtatttg ccagcaatgt aataggttta
     5461 tataccgtca tagtttgcgt accggcagca atacgtttaa tacctgcgag atccgcatcc
     5521 tggccagaaa ttgctacttt ccctgataaa ccttgcgcgc ttaatgcctg aattgcccca
     5581 cctgcggtgg catcgtttga ggcaactaca gcatcaattt tgttattatt ggcggttagc
     5641 gcgttttcca taattttcaa tgcgttttcc ggtaaccagc catcaaccca ttggtcacca
     5701 acgactttaa tttttccgga atcaacgtaa ggttgtaaca ctttcatttg tccggcgcgg
     5761 aacagcttag cgttgttatc taccggcgag ccgcccatca ggaagtaatt accttgcgga
     5821 acaatatcga ccagggcttt tgcctgcagt tcaccgactt tttcgttatc gaaagaaata
     5881 taaaaatcga tatccgcatc gttaatcata cggtcgtaag ctaatacttt aatgccttct
     5941 tgtttggctt cttttacaac gttacttaat acctgaccgt tatacggaat aatgacaaga
     6001 acatcgacac cccggtttat catgttttca atctgcgaca tttgtgtttc ttcattgcca
     6061 tttgcagact gtacaaatac tttcgcgccg agagattctg cctttttcac aaagatatct
     6121 cgatcttttt gccagcgttc aagacggaga tcatcaatcg ccatacctat tttgacttct
     6181 ttggcgtgtg cagcaacgtt ggtaagcagg agtgaggtgc aaagggtgag tagaatgttc
     6241 tttattttca tggtgtaggg ccttctgtag ttagaggaca gctttaataa gtaacaatca
     6301 ccgcgataaa cgtaaccaat ttttagcaac taaacagggg aaaacaatta cagattttta
     6361 tctttcgatt acgatttttg gtttatttct tgatttatga ccgagatctt acttttgttg
     6421 cgcaattgta cttattgcat ttttctcttc gaggaattac ccagtttcat cattccattt
     6481 tattttgcga gcgagcgcac acttgtgaat tatctcaata gcagtgtgaa ataacataat
     6541 tgagcaactg aaagggagtg cccaatatta cgacatcatc catcacccgc ggcattacct
     6601 gattatggag ttcaatatgc aagcctattt tgaccagctc gatcgcgttc gttatgaagg
     6661 ctcaaaatcc tcaaacccgt tagcattccg tcactacaat cccgacgaac tggtgttggg
     6721 caagcgtatg gaagagcact tgcgatttgc cgcctgctac tggcacacct tctgctggaa
     6781 cggggcggat atgtttggtg tgggggcgtt taatcgtccg tggcagcagc ctggtgaggc
     6841 actggcgttg gcgaagcgta aagcagatgt cgcatttgag tttttccaca agttacatgt
     6901 gccattttat tgcttccacg atgtggatgt ttctcctgag ggcgcgtcgt taaaagagta
     6961 catcaataat tttgcgcaaa tggttgatgt tctggcaggc aagcaagaag agagcggcgt
     7021 gaagctgctg tggggaaccg ctaactgctt tacaaaccct cgctatggcg cgggtgcggc
     7081 gacgaaccca gatccagaag tcttcagctg ggcggcaacg caagttgtta cagcgatgga
     7141 agcaacccat aaattgggtg gtgaaaacta tgtcctgtgg ggcggtcgtg aaggttacga
     7201 aacgctgtta aataccgatt tgcgtcagga gcgtgaacaa ctgggccgct ttatgcaaat
     7261 ggtggttgag cataaacata aaatcggctt ccagggcacg ttgcttatcg aaccgaaacc
     7321 gcaagaaccg actaaacatc aatatgatta cgatgctgcg acagtctatg gcttcctgaa
     7381 acagtttggt ctggaaaaag agattaaact gaacattgaa gctaaccacg cgacgctggc
     7441 aggtcactct ttccatcatg aaatagccac cgccattgcg cttggcctgt tcggttctgt
     7501 cgacgccaac cgtggcgatg cgcaactggg ctgggacacc gaccagttcc cgaacagtgt
     7561 ggaagagaat gcgctggtga tgtatgaaat tctcaaagca ggcggtttca ccaccggtgg
     7621 tctgaacttc gatgccaaag tacgtcgtca aagtactgat aaatatgatc tgttttacgg
     7681 tcatatcggc gcgatggata cgatggcact ggcgctgaaa attgcagcgt gcatgattga
     7741 agatggcgag ctggataaac gcatcgcgca gcgttattcc ggctggaata gcgaattggg
     7801 ccagcaaatc ctgaaaggcc aaatgtcact ggcagattta gccaaatatg ctcaggaaca
     7861 taatttgtct ccggtgcatc agagtggtcg ccaggagcaa ctggaaaatc tggtaaatca
     7921 ttatctgttc gacaaataac ggctaactgt gcagtccgtt ggcccggtta tcggtagcga
     7981 taccgggcat ttttttaagg aacgatcgat atgtatatcg ggatagatct tggcacctcg
     8041 ggcgtaaaag ttattttgct caacgagcag ggtgaggtgg ttgcttcgca aacggaaaag
     8101 ctgaccgttt cgcgcccgca tccactctgg tcggaacaag acccggaaca gtggtggcag
     8161 gcaactgatc gcgcaatgaa agctctgggc gatcagcatt ctctgcagga cgttaaagca
     8221 ttgggtattg ccggccagat gcacggagca accttactgg atgctcaaca acgggtattg
     8281 cgccctgcca ttttgtggaa cgacgggcgc tgtgcgcaag agtgcgcttt gctggaagcg
     8341 cgagttccgc tatcgcgggt gattaccggc aacctgatga tgcccggatt tactgcgcct
     8401 aaattgctat gggttcagcg gcatgagccg gagatattcc gtcaaatcga caaagtatta
     8461 ttaccgaaag attacttgcg tctgcgtatg acgggggagt ttgccagcga tatgtctgac
     8521 gcagctggca ccatgtggct ggatgtcgca aagcgtgact ggagtgacgt catgctgcag
     8581 gcttgcgact tatctcgtga ccagatgccc gcattatacg aaggcagcga aattactggt
     8641 gctttgttac ctgaagttgc gaaagcgtgg ggtatggcga cggtgccagt tgtcgcaggc
     8701 ggtggcgaca atgcagctgg tgcagttggt gtgggaatgg ttgatgctaa tcaggcaatg
     8761 ttatcgctgg ggacgtcggg ggtctatttt gctgtcagcg aagggttctt aagcaagcca
     8821 gaaagcgccg tacacagctt ttgccatgcg ctaccgcagc gttggcattt aatgtctgta
     8881 atgctgagtg cagcgtcgtg tctggattgg gccgcgaaat taaccggcct gagcaatgtc
     8941 ccagctttaa tcgctgcagc tcaacaggct gatgaaagtg ccgagccagt ttggtttctg
     9001 ccttatcttt ccggcgagcg tacaccacac aataatcccc aggcgaaagg ggttttcttt
     9061 ggtttgactc atcaacatgg ccccaatgaa ctggcgcgag cagtgctgga aggcgtgggt
     9121 tatgcgctgg cagatggcat ggatgtcgtg catgcctgcg gcattaaacc gcaaagtgtt
     9181 acgttgattg gtggcggggc gcgtagtgag tactggcgtc agatgctggc ggatatcagc
     9241 ggtcagcagc tcgattaccg tacggggggg gatgtggggc cagcactggg cgcagcaagg
     9301 ctggcgcaga ttgcggcgaa tccagagaaa tcgctcattg agttgttgcc gcaactaccg
     9361 ttagaacagt cgcatctacc agatgcgcag cgttatgccg cttatcagcc acgacgtgaa
     9421 acattccgtc gcctctatca gcaacttctg ccattaatgg cgtaaacgtt atcccctgcc
     9481 tgaccgggtg ggggataatt cacatctata tatctcagca attaattaat atttagtatg
     9541 aatttattct gaaaatcatt tgttaatggc atttttcagt tttgtctttc gttggttact
     9601 cgtaatgtat cgctggtaga tatggagatc gttatgaaaa cctcaaaaac tgtggcaaaa
     9661 ctattatttg ttgtcggggc gctggtttat ctggttgggc tatggatctc atgcccattg
     9721 ttgagtggaa aaggctattt tcttggcgtg ttaatgacag caacttttgg caactatgca
     9781 tatcttcgcg cagaaaaact cgggcaactg gataattttt ttacccatat ttgccagtta
     9841 gttgcgttaa tcactatcgg tctcttgttt atcggtgttt taaatgcacc tatcaatgct
     9901 tatgaaatgg tgatctaccc catcgccttt tttgtctgct tgtttggtca aatgcgtttg
     9961 tttcgctcgg tatgagcaac ataaagctct tacacattca ggaatgaaag gaatactgtg
    10021 atggacaaca aaatatcaac ctattcaccg gcctttagta ttgtgtcatg gatagctctc
    10081 gttggtggta tcgttaccta tctgttaggg ctatggaatg cagagatgca gttaaatgaa
    10141 aaaggatatt attttgccgt actggtatta ggactgtttt ctgcggcgtc ttatcaaaag
    10201 accgttcggg acaagtatga aggcataccg accacttcca tttattatat gacctgcctg
    10261 actgtcttta ttatctctgt tgcattactg atggtaggtc tgtggaatgc gacattatta
    10321 ctcagcgaaa aaggttttta tggactggct ttcttcttaa gcttgtttgg tgcagtagcg
    10381 gtgcagagaa atattcgtga tgccggaata aacccaccaa aagaaacaca ggttacccag
    10441 gaagaataca gcgaataact cacgtaagcc cggtcagtcc aatgtgaccg ggcttttact
    10501 taactcacta atctgtttct gtcgattcgt tgtaccagca tagaaagtaa caaactcgct
    10561 gccaacgtcg cgcaaaaaat ccaaataata tccagtattg gccaattttt aagctcaatt
    10621 ccccgggtgc gcagcgcatg gataatcaag gcgtggaatc cgtatatacc caacgaatgg
    10681 cgggagatta agccaagtcc gcgaatggta cgcgtatcca gcgtgttttt aaccagagtc
    10741 aatagcgcga ttgcgcagat aaaaaccatc ggcccacagt aaggatacca ggtatcggca
    10801 aaatttccgc gccactgcaa ttcatataat gtcccgcgag agataataaa aactcccgtc
    10861 gcaaacagtg cggcgctcac ccacgacagt gctttatgct gtgtgtccat catccctaaa
    10921 gcgcggccca acatgccata cagaatgtag taaaaagtat cgccattgat atataagtta
    10981 attggcagcc attcaaaacc gtcgattttc tgcggcacgg tgtttgggtt agcgatgata
    11041 ccaatcacca ccattagcac cagcaacatt tttccgccga cgttcttcac ctgaatcagc
    11101 ggtgaaacca gataaatcac cgcaatcgcg aagaaaaacc ataagtggta aaacactggc
    11161 ttttgcagca ggtttttcag cgctaactcc acattaatgg aggtaaacag tgcaatgtag
    11221 agcagcgcga ttgcgctata aaaaaacaga cataagccga tacgcaagaa atggcgcggc
    11281 tgggcgctgc gttcgccaaa aaagagatag ccggaaatca tgaaaaatag cggcacgctg
    11341 acacgagagg cagagttcag aacattggcg atatcccagg tgacggggct aacactatga
    11401 gcattggtca cataccaggt agtggtgtga atcatcacca ccattaaaca cgctatccct
    11461 cgcaggttat caatccagta aattttgggc tgcatctgtg tctctgtatc tggttaaaaa
    11521 aagtctgacc gataaatcat tcagttggcg cactggaata atctgagttt taacactaca
    11581 gcttatagag gcttaaggaa attcgtaaga tatcagccac tataccgata taaataataa
    11641 gactcacctg caaaccagac ggtaatttaa tgatgatgaa ctctttcttt ccggcaatgg
    11701 cgcttatggt gctagtgggt tgttctacac cgtcacccgt gcagaaagca caacgggtaa
    11761 aggttgatcc tctgcgttcg ttgaatatgg aagcgttatg caaggatcag gcggcaaaac
    11821 gttataacac cggcgagcaa aaaatcgacg tcaccgcctt cgaacagttc cagggaagct
    11881 atgaaatgcg cggttatacc ttccgtaaag agcagtttgt ctgttctttt gacgcagatg
    11941 gccatttttt gcatctttcc atgcgttaag ccctgctttt tcccgtttca tactgtatat
    12001 cttccatcca gcgggtatac tgatcccttc ctttaaatcc acacgtatcc agcacgaaat
    12061 aatatgcaaa agtttgatac caggaccttc cagggcttga tcctgacctt acaggattac
    12121 tgggctcgcc agggctgcac cattgttcaa ccattggaca tggaagtcgg cgcgggaacc
    12181 tctcacccaa tgacctgtct gcgcgcgctg gggccagaac cgatggcggc tgcttatgtt
    12241 cagccttctc gtcgcccgac cgatggtcgc tacggcgaaa accccaaccg tttacagcac
    12301 tactatcagt tccaggtggt cattaagcca tcgccggaca atattcagga gctgtacctt
    12361 ggttctctga aagagctggg catggacccg actatccacg atatccgttt cgtggaagat
    12421 aactgggaaa acccgacgct gggtgcctgg ggactgggct gggaagtgtg gctaaacggc
    12481 atggaagtga cgcagttcac ttacttccag caggttggtg gtctggagtg taaaccggtt
    12541 accggcgaga tcacctacgg tctggaacgt ctggccatgt acattcaggg cgtagacagc
    12601 gtttacgacc tggtctggag cgacggcccg ctgggtaaaa ccacctacgg cgacgtgttc
    12661 catcagaacg aagtggagca gtccacttac aacttcgaat acgcggatgt ggacttcctg
    12721 ttcacctgct ttgagcagta cgagaaagaa gcgcagcagc tgctggcgcg ggaaaatccg
    12781 ctgccgctgc cagcttacga gcgtattctg aaagccgccc acagcttcaa cctgctggat
    12841 gcgcgtaaag ccatctccgt caccgagcgt cagcgctaca ttctgcgcat tcgcaccctg
    12901 accaaagcag tggcagaagc atattacgct tcccgtgaag ccctaggctt cccgatgtgc
    12961 aacaaagata agtaagaggc ggctatgtct gagaaaactt ttctggtgga aatcggcact
    13021 gaagagctgc caccaaaagc actgcgcagc ctggcagagt cctttgctgc gaactttact
    13081 gcggagctgg ataacgcagg cctcgcacac ggcaccgttc aatggtttgc tgctccgcgt
    13141 cgtctggcgc tgaaagtagc taacctggcg gaagcgcaac cggatcgtga aatcgaaaaa
    13201 cgcggcccgg cgattgccca ggcgttcgac gctgaaggca aaccgagcaa agcggcagaa
    13261 ggttgggcgc gtggttgcgg tattaccgtt gaccaggctg agcgtctgac taccgataaa
    13321 ggcgaatggc tgctgtatcg cgcccatgtg aagggcgaaa gcactgaagc actgctgccg
    13381 aatatggttg cgacttcgct ggcgaaactg ccgattccga aactgatgcg ctggggtgca
    13441 agcgacgtgc acttcgtgcg tccggtgcac accgtgaccc tgctgctggg cgacaaagtc
    13501 attcctgcaa ccattctggg cattcagtcc gatcgcgtga ttcgcggcca ccgctttatg
    13561 ggcgagccgg aattcaccat cgataacgcc gatcagtatc cggaaattct gcgtgagcgt
    13621 gggaaagtca tcgccgatta cgaagaacgt aaggcgaaga ttaaagccga tgccgaagaa
    13681 gcagcgcgta agattggcgg taacgctgac ttaagcgaaa gcctgctgga agaagtggct
    13741 tcgctggtgg agtggccggt cgttctaacc gcgaaatttg aagagaaatt cctcgcggtg
    13801 ccgtctgaag cactggttta caccatgaaa ggtgaccaga agtacttccc ggtgtatgcg
    13861 aacgacggca aactgctgcc gaactttatc ttcgttgcca acatcgaatc gaaagatccg
    13921 cagcaaatta tctctggtaa cgagaaagtc gttcgtccac gtctggctga tgccgagttc
    13981 ttcttcaaca ccgaccgtaa aaaacgtctg gaagataacc tgccgcgcct gcaaaccgtg
    14041 ctgttccagc aacagctggg tacgctgcgt gacaagactg accgcatcca ggcgctggct
    14101 ggctggatcg ccgaacagat tggcgctgac gttaaccacg caacccgtgc gggcctgctg
    14161 tctaagtgtg acctgatgac caacatggtc ttcgagttca ccgacaccca aggcgttatg
    14221 gggatgcact acgcgcgtca cgatggcgaa gcggaagacg tagccgtcgc gctgaatgag
    14281 cagtatcagc cgcgctttgc cggtgatgac ctgccgtcca acccggtagc ttgtgcgctg
    14341 gcgattgctg ataagatgga tactctggcg ggtatcttcg gtatcggtca gcatccgaaa
    14401 ggcgacaaag acccgtttgc gctgcgtcgt gccgcacttg gcgtgctgcg aattatcgtt
    14461 gagaagaacc tcaaccttga tctgcaaacg ctgaccgaag aagcggtgcg tctgtatggc
    14521 gataagctga ctaatgccaa cgtagttgat gatgttatcg actttatgct cggtcgcttc
    14581 cgcgcctggt atcaggacga aggttatacc gttgacacca tccaggcggt actggcgcgt
    14641 cgtccgactc gtccggctga tttcgatgcc cgcatgaaag cggtatcgca cttccgtacc
    14701 ctggaagcag ctgcagcact ggcggcggcg aacaaacgtg tatctaacat cctggcgaaa
    14761 tctgacgaag tgctgagcga tcgcgtgaat gcctctactc tgaaagagcc ggaagaaatt
    14821 aaactggcga tgcaggttgt ggtgctacgt gacaagctgg agccgtactt tgcggaaggt
    14881 cgttaccagg atgcgctggt cgaactggca gagctgcgtg aaccagtcga tgccttcttc
    14941 gataaagtga tggtcatggt tgatgacaaa gaattgcgta tcaaccgtct gaccatgctg
    15001 gagaaactgc gcgaactgtt cctgcgcgtt gcggatattt cgctgttgca gtaataacgc
    15061 cgttattaaa aagcctgcca tctggcaggc tttttttatc gctaaataat acagcaacct
    15121 ttaataatct tctgctgaat aaagattatc tcatatatta attttatgag atttttttag
    15181 gattatatca aggagaagaa acaaacttat taagctagaa tagccacggg tgcttgagac
    15241 tgtttgtctc aggtattcac cgaaaggcag acagagaaaa gccccacctg actataaatc
    15301 aaagtgaggc ccctctatgt atagacacca taaggttagc ctcttactcg ttggaaatca
    15361 acacaggggg ctgggaatgc cgcagaaata tagattgctt tctttaatag tgatttgttt
    15421 cacgctttta tttttcacct ggatgataag agattcactg tgtgaattgc atattaaaca
    15481 ggggagttat gagctggcgg catttttagc ctgcaatttg aaagagtaag agtcttcggc
    15541 ggaaagttat tcccgccttt cttacggcgt tgcgcattct cattgcaccc aaatttattt
    15601 ttcacaaaaa taatactaga ttttattacg cgatcgatta tttatttcct gaaaaacaaa
    15661 taaaaaaatc cccgccaaat ggcagggatc ttagattctg tgcttttaag cagagattac
    15721 aggctggtta cgttgccagc tgccgggcct ttagcgccgc tttcgatggt gaaggacact
    15781 ttctgacctt cgtccagaga tttgtaacca tcgttctgga tagcagagaa gtgtacgaac
    15841 acgtctttag agccatcgtc aggagtgatg aagccgaagc ctttgtcagc gttgaaccat
    15901 tttacgatac cagtcatttt accggacata gtgtattacc tttaataatt aagtgtgcct
    15961 ttcggcgata tggcgtgctt tacagatttt gaagcgttaa aggaatgtgc actacgaggg
    16021 gtatcaacga taactcttga agggacttgc cttactacac tgctttaatg gtctgtacgt
    16081 caaaccgttg atgtgcatta agccacgcat tggcgggtga tgcaacaatt atttttcata
    16141 tttatgatta atcggttgga aaacggtcct gtcatcagga ccgtaaacag caataaagtg
    16201 gataaaagtc tattccatca actgcttact taatgccggg ttggcttgaa tcaaacgcat
    16261 caattttagt tcggcacttg aaggcttcac gcgtctggat tcccattcct ttaccatggc
    16321 gactgatacg cccaaaaccc gggcgaaatc atcgattttt aatcctgtcc cttttcgtaa
    16381 ctgctcaatt tcggtacagg acgttgttct gtgcgtcagg gtaattttct gcgtttcatc
    16441 tttaaaaaca atctgttcca ggctgctcaa cagctcatgc attggatctt tatattccat
    16501 tgaaaactcc tcaaatcaca ctgcgggatc gtgaattgca tcgaagctca ttaagaatag
    16561 tcgggaaaag cagacacagc ggggtgaagt cagggcatgc tctggctatg attcgattca
    16621 gccagtcatt tagcgtgact tatagccacc taacgatctg ataaatgccg atatatctct
    16681 aaccactcct ggtttgtaaa gatttcgaaa atctaacctt ctgcgaaggc gtaaatctct
    16741 ggctacaaaa aaggcaacgg caatatcatt ttttaacaat gaaacaacaa attaaccttg
    16801 tttcattttc tccatgattt taccaggaag attacgtaga aagggtatcc tgcaagcctg
    16861 tcctggacta tccttgtcac gtcagacacg cgtgtgtcgt tgtgcgcttt ttttgggtga
    16921 aaggagtaag aaaatggcga caggaaagtc ctgctctcgc tggtttgcgc ctcttgcggc
    16981 gttattaatg gtagttagcc tgagtgggtg ttttgataaa gaaggcgatc agcgtaaagc
    17041 gtttatcgac ttcctgcaga atacagtgat gcgtagcggt gaacgtctac caaccctgac
    17101 tgccgatcag aaaaaacagt ttggtccttt tgtctctgat tacgcgattc tgtatggtta
    17161 ttctcagcag gtgaatcagg cgatggattc cggtctgcgc ccggttgtag acagcgttaa
    17221 cgcaattcgc gtaccgcagg attacgttac gcaaagcggt ccgctgcgtg aaatgaatgg
    17281 ttcgctgggt gtgctggcgc aacaactgca aaatgcgaag ttgcaggccg atgctgcgca
    17341 ctctgcgcta aaacagagtg atgacctgaa accggtcttt gatcaggcat tcaccaaagt
    17401 ggtaacgacg ccagctgatg cattgcaacc gttgattccg gcggcacaaa ctttcacgca
    17461 acaactggtg atggttggag actacattgc ccaacaaggt actcaggtga gctttgtagc
    17521 aaatggcatc cagttcccga cttcacagca ggcaagtgaa tataacaaac tgattgcgcc
    17581 attaccggca caacatcagg cgtttaatca ggcctggact acagcagtta ctgcaaccca
    17641 ataaagagta aaagcccgag cgattatcgg gctttactcc acgcagtcgc ggcttagtcc
    17701 gcgacgtgcg gattcacaca gttcttctca acctttcctt gtaacgcatc aatcaaatta
    17761 tccacggcac aggcggccat gccataacgc gtctcatggg tggcagatcc aatatgcggt
    17821 actgcgacga cgttggccat tgagagcaac ggcgaatcta cggaaagtgg ctcttgttcg
    17881 aagacatcca gcccggcggc gtgaatttcc cctttctgca atgctgcgat cagtgcattt
    17941 tcgtcaacca ccgggccacg tccggcatta atgaaaatgg cggaggattt cattttggcg
    18001 aattgttctg cgccaaacag atgatgcgtc tcatcagtta acggcaggat caggcaaacg
    18061 aaatctgact cttgtaacag tgtatccaaa tcgcagtagc gggcgttgaa gcgttcttct
    18121 gcttctttat ggtggcggcg cgcgttatag aggatgggca tgttgaagcc aaagtgcgca
    18181 cgttgtgcca gcgccatacc gatccgtccc atcccgacaa tgcccagtgt tttatggtga
    18241 acgtcagtgc cgtaccagtc cgggcctatg ctcgcggtcc attcgcctgc ttttacccgt
    18301 tctgctacct ccacaacccg acgagcggta gacaacacca gcgccatcag cgtatcggcg
    18361 acggtttctg ttaagacggt tggcgtgtgc atcagcagaa tttttcgggc ggtaagcgca
    18421 tcgacatcaa aattgtcata gccgacggag atcgttgatg tggcacgcag tttcggcatt
    18481 ttttccagca atgcggcatc aacattctcg tttgaaccca gtaaaccttc agcttcggca
    18541 aaaattgctg cattttgttc gacggtttgt gggctgaggt ttgccacctg gtgaacggtg
    18601 aaatgctctt gcaggcgttg cagtaaatca tcaggtaagg ctttgtagag gataacggac
    18661 ggcttcatgc ttctctccat tcactgataa ggcgatcacc ttagcctgaa atatgatgaa
    18721 ggaaaactga agaatgccag gtgatttctg catcacctgg catgaaaggg attacagcgg
    18781 gcttaaggta atttctacac ggcggttttg cgccttacct tctgcggtgc tgttgctggc
    18841 gattgggtta gccgggccaa ggccctgagt acggatgcgg ctggcgtcca cgccctgggt
    18901 gatcaacgcg ctggcaacgg aatccgcacg ctgctgggag agacgcatgt tcagatcgtg
    18961 accacccgtg ctgtcagtat aaccaatcac gttaaccgcc gttttcggat actctttcag
    19021 caccattgcc acgccggtca gggtgttagc gcccgccggt ttcagggttg cgctgctgct
    19081 gtcgaaggtc acattgttcg gcatattgag gataatgtta tccccgctgc gggttacgct
    19141 aacaccagtg ccgcgcattt tgtcacgcag cttcgcttcc tgcacatcca tgtaataacc
    19201 cacgccgccg cccagagctg cgcctgctgc tgcgccaatc agcgcgcctt taccgcgatc
    19261 tttcttcgaa gaagagagcg caccaatacc cgcgcccacg agagagccca gacctgcgcc
    19321 gatagcagat ttacctgctt cgcgttcgcc ggtgtaaggg ttagttgtgc agccagatac
    19381 cgccagagcg ccactcacta cggcggcaat aagataaaca cgtttcttca ttgttaatcc
    19441 ttaataacct ttttattctt tgccacgggt tccgtggcgg gagattatgc cgcgtgaaca
    19501 tgaagattat tcctgggaat actcggaaat ttgtaagtaa tatttaactg ctcaatacat
    19561 ctaacctttc aggagtcttc ggtttgacca actcatcctc acgatattcc gttctgactg
    19621 ccgcccattg ggggcccatg ctggttgaaa ccgacggcga aaccgtgttt agctcgcgtg
    19681 gcgcgttagc cacaggaatg gaaaactcct tgcagagcgc ggttcgtgac caggttcaca
    19741 gcaatacgcg ggtacgattt ccaatggtgc gcaaaggctt tctcgcgtca ccggaaaacc
    19801 cgcaaggcat tcgtgggcag gatgaatttg ttcgcgtgag ttgggatgag gcgctggatc
    19861 ttattcacca acaacataaa cgcattcgtg aggcttatgg tccggcatcg atttttgctg
    19921 gttcctacgg ctggcgttca aacggcgtgc tgcataaggc ctcgacatta ttacaacgct
    19981 atatggcgct ggcaggcggt tataccgggc atctggggga ttattcgacc ggcgcggcac
    20041 aggcgatcat gccgtatgtc gtgggtggta gtgaagttta tcaacagcag accagttggc
    20101 cgctggtgct ggaacatagc gatgtcgtgg tgctgtggag tgctaaccca ctcaatacgc
    20161 tgaaaattgc gtggaatgca tccgatgagc aggggctttc ttacttttct gcactgcgtg
    20221 acagcgggaa aaagctgatc tgcattgatc caatgcgatc ggaaaccgtc gatttctttg
    20281 gcgataaaat ggagtgggtg gcaccgcaca tgggcactga tgttgcgctg atgctgggga
    20341 tcgcccatac gctggtggaa aatggttggc acgacgaagc gtttctggcg cgttgcacca
    20401 ccggttatgc cgtcttcgcc tcttatttgc tgggcgagag tgacggaata gcgaaaaccg
    20461 ccgaatgggc agcagagatt tgtggtgtta acgcagagaa aatccgcgag ctggcggcta
    20521 ttttccacca aaataccacc atgctgatgg ctggctgggg aatgcagcgc caacagtttg
    20581 gtgagcaaaa acactggatg atcgtcacgc tggcagcaat gttggggcaa atcggcacac
    20641 ccggcggcgg ttttggtctt tcttaccatt ttgccaatgg tggtaacccc acgcggcgcg
    20701 ctgcggtact ctcttccatg cagggcagct tgccgggtgg ctgcgatgcg gtagataaaa
    20761 tccctgtcgc ccgcattgtt gaagcactgg aaaaccccgg tggcgcatat caacacaacg
    20821 gtatggaccg acatttcccg gatattcgtt ttatctggtg ggcgggcggt gccaacttta
    20881 ctcatcatca ggataccaat cgcctgatcc gtgcctggca aaaaccggag ctggtggtga
    20941 tctctgaatg cttctggacg gcggcggcaa aacacgcgga tatcgttctg cctgcgacta
    21001 cctcttttga gcgtaatgat ctcaccatga ccggtgatta cagtaatcag catctggtgc
    21061 cgatgaagca agtggtgccg ccacgctatg aagcgcgtaa tgattttgat gtttttgccg
    21121 agttaagtga acgctgggag aagggcggtt atgcacgttt tacggaagga aaaagtgagc
    21181 tgcaatggct ggaaacgttt tataacgttg cccggcagcg cggggcaagc cagcaggttg
    21241 aattgccgcc atttactgag ttctggcaag ccaaccagtt aattgagatg ccggaaaacc
    21301 cggacagcga gcggtttatt cgcttcgctg atttttgccg cgatccgcag gcgcatccat
    21361 taaaaaccgc cagcggtaag attgaaatct tctcgcagcg tattgccgat tacgcttatc
    21421 cggattgccc ggggcatcca atgtggctgg tgccggacga atggcagggc aatgccgaac
    21481 cggaacagtt acaggtactt tctgctcatc cggcgcatcg tctgcacagc cagctgaatt
    21541 acagttctct gcgcgaattg tacgcggtgg caaaccgtga gcctgtcacc attcatcctg
    21601 acgatgccca ggcgcgcggc atacaagatg gcgatactgt tcggctgtgg aacgcacgcg
    21661 ggcaaattct tgccggagcg gtcattagcg agggaattaa acctggcgtg atttgcattc
    21721 acgaaggggc atggccggat ctggatttaa ccgctgacgg tatttgtaaa aacggcgcag
    21781 tgaacgtgct gaccaaagat ctccccagct cgcggctggg gaatggctgt gcgggtaata
    21841 cggcgctggc atggctagaa aaatacaacg gtccggaact gacacttaca gcgtttgaac
    21901 caccggccag ctcataatcc aggtgggtag ttgggtttca tcctgccatg cgcaatcgac
    21961 aatgtgaaaa ccctgcgcct ggtaaaaatc tatcgccggt tgattttttt gataaacctc
    22021 cagcatcaga tggggatagc gctgctgcac atactgcatc agcgccttac caataccgcg
    22081 tctgacggcc ttcggtgcga caaacattgc tgccagaaat cggccttcca taatgctgac
    22141 aaaaccgaga agcttaccgt cttcttccca gacccagttt ttcgcattgg caagatacgc
    22201 atcccgcacc agcggaatgc actcgcgcca gtaattcact tttataaagg gatgccccca
    22261 ggttgtactt tccagccaca gttcgaggat cgcggggagt tctgaacgtt gcgcttcccg
    22321 aatcatggtt tatttcccgg atagcaacag cagccaacca catgatcatt caccagccca
    22381 catgcctgca taaaggagta acagattgtg gtaccgacaa acttaaaacc acgttttttc
    22441 agtgccttag atagggcgtc ggaggcgggc gtagatgtgg ggatttcgct caacgttgtg
    22501 gcttgtgtca cctgtggctg atgatttaca aacgaccaga caaagtcggc aaacggttcg
    22561 ccgttctgtt ccatttgcag gtacgcccgc gcattaccaa taattgcctg aattttcccg
    22621 cgatggcgga taatcccggc gtcctgtacc agtctttcga catcctcttc ctgcattgcg
    22681 gcaaccttca ccggatcgaa ctgatgaaag caggcgcgat agttttcgcg ttttttgagg
    22741 acggtgatcc acgataaccc agcctgctgc ccttccaggc agatcatttc gaacagtttt
    22801 ttcctgtcag tttcaggcac gccccactca ttatcatggt aggcaatata aagcgggtcc
    22861 tgactcaccc agccgcaacg ttccatactt tccctcgcta tgtacccgat cgtaaaaatt
    22921 tcagccgctt ttcttgacgc atcggtgaaa aagtcaatat ttaatacagg gctactatgc
    22981 ccgatatcct tcatacaatg acaataatat tgccgaattc ggctcttccc tggagtcgtg
    23041 ttaatgatca taaaaaaaag cggtggtcgc tggcagctaa gcctgctggc gagtgtggta
    23101 atcagtgcct tttttctcaa cacagcttac gcctggcaac aagaatatat cgttgatacg
    23161 caacccgggc attccacaga gcgttacacc tgggatagtg atcatcaacc tgattacaac
    23221 gatattttgt cgcaacgtat tcaaagtagc caaagggcgc tgggactgga agtcaatctg
    23281 gcggaagaaa cccctgtgga tgtgaccagc agtatgagta tgggctggaa ttttccttta
    23341 tatgaacagg ttacaactgg cccggtcgcg gcattacatt acgatggcac aaccacctcg
    23401 atgtataacg agtttggcga cagtactacc acgctgaccg atccgttatg gcatgccagc
    23461 gtgagtacct taggctggcg tgttgactcc cggcttggcg atctccgacc ctgggcgcaa
    23521 atcagctata accagcaatt tggcgagaat atctggaagg cgcaatcagg cctgagccgg
    23581 atgacggcga caaaccagaa cggcaactgg ctggatgtca ccgtaggcgc tgatatgttg
    23641 ctcaatcaaa atattgccgc ctatgccgcg ctatctcagg cagaaaatac cactaacaat
    23701 agcgattatc tgtatacgat gggggttagt gccagatttt aacgtaacaa tcacaattga
    23761 aaccattgaa taacaatagt tgtggcgata gtgggtgcta acttaccaaa taataaattt
    23821 ggtgaataat tgtcgcgtca ttcattcctg aactaaggca tttcattccg ttctgatggc
    23881 atttcatgcc gtttttcccc aggcataaag tgcacttcgt tatggttgtc ggcagagatt
    23941 tttccttttt attactgcag gaatactgcc atgacacctt caaattatca gcgtacccgc
    24001 tggttgacac tcatcggtac tatcattacc cagtttgcgc tggggtcggt ttatacctgg
    24061 agcctgttta acggcgcgct ttccgccaag ctggatgcgc cggtaagcca ggtcgctttc
    24121 tctttcggct tgttaagtct ggggctggca atatcgtctt ctgttgcggg caaattgcag
    24181 gaacgttttg gtgttaaacg cgtcaccatc gcttccggca ttttgctggg attagggttc
    24241 ttcctgacag cgcattctga caacctgatg atgctgtggt taagcgccgg tgtgctggtc
    24301 ggtctggcgg atggcgcggg ttacctgctg acgctctcta actgcgtgaa atggttcccg
    24361 gagcgtaaag gtctgatctc cgcgttcgct atcggttctt atggtctggg tagcctgggt
    24421 ttcaaattta tcgacacgca gctgctggaa acggtcggtc tggaaaaaac ctttgtgatt
    24481 tggggagcga ttgcgctggt gatgattgtt ttcggcgcaa cgttaatgaa agacgcacca
    24541 aaacaggaag tgaaaaccag caatggtgtg gtggagaaag attacacgct ggcagagtcg
    24601 atgcgtaaac cgcagtactg gatgttagcg gtaatgttcc tgactgcctg catgagcggc
    24661 ctgtacgtga ttggggtagc gaaagatatc gcccaaagtc tggcacacct tgatgtggtt
    24721 tctgcagcca atgcagtcac tgttatttcc atcgccaacc tttcaggtcg tctggtgctg
    24781 ggcattctgt ctgacaaaat cgcccgtatc cgtgttatca ccattggtca ggtaatttcg
    24841 ctggtgggta tggcggccct gctgtttgca ccattgaatg cagtgacgtt ctttgcagcg
    24901 attgcctgcg tggcatttaa ctttggcggc actattaccg tctttccgtc actggtcagt
    24961 gagttcttcg gcctcaataa cctggcgaaa aactacggtg tgatttacct cggtttcggt
    25021 atcggtagca tttgtggttc gattatcgcc tcactgtttg gcggcttcta tgtgaagttc
    25081 tacgtcatct ttgctctgct gattctgtca ttggcgcttt ctacgacgat tcgtcagcca
    25141 gagcagaaag tattgcgtga ggcgcatatt tagccagaaa ctacaggttt tgaacgaaag
    25201 ggggaggaaa tcctcctttt ttattgcatt ttaaagaa
//
LOCUS       DAZFAB010000067        24586 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZFAB010000067 DAZFAB010000000
VERSION     DAZFAB010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24586
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.067"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(113..2686)
                     /gene="clpB"
                     /locus_tag="ACHJWA_003034"
     CDS             complement(113..2686)
                     /gene="clpB"
                     /locus_tag="ACHJWA_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFO4309585.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2816..3547)
                     /gene="yfiH"
                     /locus_tag="ACHJWA_003035"
     CDS             complement(2816..3547)
                     /gene="yfiH"
                     /locus_tag="ACHJWA_003035"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFO4309586.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLNLTGEPYASKRADASYSNTPGRVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAEASTAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3544..4524)
                     /gene="rluD"
                     /locus_tag="ACHJWA_003036"
     CDS             complement(3544..4524)
                     /gene="rluD"
                     /locus_tag="ACHJWA_003036"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFO4309587.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKLCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            4659..5396
                     /gene="bamD"
                     /locus_tag="ACHJWA_003037"
     CDS             4659..5396
                     /gene="bamD"
                     /locus_tag="ACHJWA_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFO4309588.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5667..6008
                     /gene="raiA"
                     /locus_tag="ACHJWA_003038"
     CDS             5667..6008
                     /gene="raiA"
                     /locus_tag="ACHJWA_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFO4309589.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6111..6236
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6112..6159
                     /gene="pheL"
                     /locus_tag="ACHJWA_003039"
     CDS             6112..6159
                     /gene="pheL"
                     /locus_tag="ACHJWA_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFO4309590.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6258..7418
                     /gene="pheA"
                     /locus_tag="ACHJWA_003040"
     CDS             6258..7418
                     /gene="pheA"
                     /locus_tag="ACHJWA_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFO4309591.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7461..8582)
                     /gene="tyrA"
                     /locus_tag="ACHJWA_003041"
     CDS             complement(7461..8582)
                     /gene="tyrA"
                     /locus_tag="ACHJWA_003041"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFO4309592.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPALRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8593..9663)
                     /gene="aroF"
                     /locus_tag="ACHJWA_003042"
     CDS             complement(8593..9663)
                     /gene="aroF"
                     /locus_tag="ACHJWA_003042"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFO4309593.1"
                     /translation="MQKDALNNVHITDEQVLMTPAQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            9873..10238
                     /gene="yfiL"
                     /locus_tag="ACHJWA_003043"
     CDS             9873..10238
                     /gene="yfiL"
                     /locus_tag="ACHJWA_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFO4309594.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASAIRLN"
     gene            10388..10906
                     /gene="yfiR"
                     /locus_tag="ACHJWA_003044"
     CDS             10388..10906
                     /gene="yfiR"
                     /locus_tag="ACHJWA_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFO4309595.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            10896..12122
                     /gene="dgcN"
                     /locus_tag="ACHJWA_003045"
     CDS             10896..12122
                     /gene="dgcN"
                     /locus_tag="ACHJWA_003045"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFO4309596.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLP
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSAA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYGVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIAHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            12138..12620
                     /gene="yfiB"
                     /locus_tag="ACHJWA_003046"
     CDS             12138..12620
                     /gene="yfiB"
                     /locus_tag="ACHJWA_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO4309597.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(12697..13044)
                     /gene="rplS"
                     /locus_tag="ACHJWA_003047"
     CDS             complement(12697..13044)
                     /gene="rplS"
                     /locus_tag="ACHJWA_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFO4309598.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(13086..13853)
                     /gene="trmD"
                     /locus_tag="ACHJWA_003048"
     CDS             complement(13086..13853)
                     /gene="trmD"
                     /locus_tag="ACHJWA_003048"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFO4309599.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(13884..14432)
                     /gene="rimM"
                     /locus_tag="ACHJWA_003049"
     CDS             complement(13884..14432)
                     /gene="rimM"
                     /locus_tag="ACHJWA_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFO4309600.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(14451..14699)
                     /gene="rpsP"
                     /locus_tag="ACHJWA_003050"
     CDS             complement(14451..14699)
                     /gene="rpsP"
                     /locus_tag="ACHJWA_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFO4309601.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(14836..16197)
                     /gene="ffh"
                     /locus_tag="ACHJWA_003051"
     CDS             complement(14836..16197)
                     /gene="ffh"
                     /locus_tag="ACHJWA_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFO4309602.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            16364..17155
                     /gene="ypjD"
                     /locus_tag="ACHJWA_003052"
     CDS             16364..17155
                     /gene="ypjD"
                     /locus_tag="ACHJWA_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFO4309603.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            17177..18463
                     /gene="yfjD"
                     /locus_tag="ACHJWA_003053"
     CDS             17177..18463
                     /gene="yfjD"
                     /locus_tag="ACHJWA_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFO4309604.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVVGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(18518..19111)
                     /gene="grpE"
                     /locus_tag="ACHJWA_003054"
     CDS             complement(18518..19111)
                     /gene="grpE"
                     /locus_tag="ACHJWA_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFO4309605.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            19234..20112
                     /gene="nadK"
                     /locus_tag="ACHJWA_003055"
     CDS             19234..20112
                     /gene="nadK"
                     /locus_tag="ACHJWA_003055"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFO4309606.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGATRTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            20198..21859
                     /gene="recN"
                     /locus_tag="ACHJWA_003056"
     CDS             20198..21859
                     /gene="recN"
                     /locus_tag="ACHJWA_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFO4309607.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     AHYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IVEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPETLPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQHYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKTRLQELARLLGGSEVTRNTLANAKELLAA"
     gene            22008..22349
                     /gene="bamE"
                     /locus_tag="ACHJWA_003057"
     CDS             22008..22349
                     /gene="bamE"
                     /locus_tag="ACHJWA_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFO4309608.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(22411..22701)
                     /gene="yfjF"
                     /locus_tag="ACHJWA_003058"
     CDS             complement(22411..22701)
                     /gene="yfjF"
                     /locus_tag="ACHJWA_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFO4309609.1"
                     /translation="MPGKIAVEVAYALPKKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTENKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(22691..23128)
                     /gene="ratA"
                     /locus_tag="ACHJWA_003059"
     CDS             complement(22691..23128)
                     /gene="ratA"
                     /locus_tag="ACHJWA_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFO4309610.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            23299..23781
                     /gene="smpB"
                     /locus_tag="ACHJWA_003060"
     CDS             23299..23781
                     /gene="smpB"
                     /locus_tag="ACHJWA_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFO4309611.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            23996..24358
                     /gene="ssrA"
                     /locus_tag="ACHJWA_003061"
     tmRNA           23996..24358
                     /gene="ssrA"
                     /locus_tag="ACHJWA_003061"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         6262 a         6489 c         5765 g         6070 t
ORIGIN      
        1 gaaaaatata aaaactttat cgttcgctca tttttcaggc aaaacatctt aaatatagtc
       61 ttttccgtct aacttataga caaaaacgag ccccgaaggg ctcgttttat cattactgga
      121 cggcgacaat ccggtcttca ttaacttcca ggcgaataac tttacccgga accaattcac
      181 cagacagtat ttgctgtgcc aacgggtttt cgatctgctg ctggatagca cgtttcaaag
      241 gacgtgcacc atagactgga tcgtaaccgt tctcgctcag cagtttcagc gcctcgtcag
      301 aaatgtggat ttcataacca cgttcttcca gacgtttgta cagacgtttc aactgaatct
      361 gcgcaatcga ggcaatgtgc tgttcgccca gcggatggaa gaccaccact tcatcgatac
      421 ggttaatgaa ctccggacgg aagttatggc ttaccacacc gagcaccagc tctttcatgt
      481 gcgcataatc cagttcaccg aagcgttcct gaatcagatc ggaaccgagg ttagaggtca
      541 taatgacgac cgtattacgg aagtcgaccg ttctcccttg cccgtcagtc agacgcccat
      601 catccagtac ctgcaacaga atgttgaaga catccggatg cgctttttcc acttcatcca
      661 gcaggatgac ggaatacgga cgacgacgca ccgcttcggt caggtagcca ccttcttcat
      721 aaccgacata tcccggaggc gcaccaacca gacgagacac cgagtgtttc tccataaact
      781 cggacatatc gatacggacc atcgcctcgt cgctatcaaa cataaagttc gccagcgcct
      841 tacaaagctc tgttttcccc acaccagttg ggccgaggaa caggaatgaa ccaatcgggc
      901 gatttggatc cgccagcccc gcacggctac gacgaatagc gttagatacc gcatcaaccg
      961 cttcgttctg accaattacg cgatggtgca gttcttgctc catacgcagc agtttttcgc
     1021 gctcgctttc catcatgcga gaaaccggaa tccccgtcca acgcgccagc acttcagcaa
     1081 tttcggcgtc ggtcacttta ttacgcaaca gacgcatagt tttgccttca agctgcgttg
     1141 cggcttccag ttgcttttcc agttccggga ttttgccgta ttgcagttca gacatccgcg
     1201 ccaagtcacc cacacggcga gcctgttcaa tagcgatttt cgcctgttcc agttccgctt
     1261 taatggtctg cgtaccagaa agcgatgcct tctctgcttt ccactcttct tctaactcgg
     1321 agtactgacg ttctttgtcg ctcagttctt cgttgagcat atccagacgt tttttactgg
     1381 cttcatcaga ctctttcatt aacgcctgtt gttccagttt gagctggatg atacggcgat
     1441 cgagtcggtc gagttcttct ggttttgagt caatctgcat acgaatgctg gatgctgctt
     1501 catcgatcag gtcgatggct ttatccggca gctgacggtc agcaatgtag cgatgagaca
     1561 acgtcgccgc tgcaacaatt gccgggtcag taatttgcac atggtggtgc aattcgtaac
     1621 gttctttcag gccacgcaga atcgcaatgg tatcttcaac agaaggctcg gcaacaaaca
     1681 ctttctggaa acgacgttcc agcgcagcat ctttttcgat gtactggcga tattcgtcaa
     1741 gcgtcgtggc acctacgcag tgcaattcac cacgcgccag cgccggtttc agcatgtttc
     1801 cggcgtccat tgcgccatcg gctttacccg cgccgaccat ggtatgtaat tcgtcgataa
     1861 acaggatgac gttgccttcc tgtttggcaa gatcgttaag cacgcctttt aaacgttctt
     1921 caaactcacc gcgatatttc gccccagcca ccagcgcgcc catatccagc gccagtaccc
     1981 ggcggccttt caacccttcc ggcacttcgc cgttgataat acgctgcgcc agaccttcaa
     2041 cgatggcagt tttaccgacg ccgggttcac caatcagtac cgggttattt ttagtacgac
     2101 gttgcagcac ctgaatggta cggcgaattt cttcatcacg accaatcacc ggatcgagtt
     2161 tgccctgttc ggctcgttcg gtaaggtcga tggtatattt tttcaaagcc tgacgttggt
     2221 cttcagcacc ttgatcgttc acgctttcac ctccacgcat ttgttcaatc gcttgagtaa
     2281 tgttggcggt ggtcgcccct gctgctttca ggatgtcggc cagcgtgccg cgagactcaa
     2341 gtgccgccag aacgaacagt tctgacgaga taaagttatc accacgtttt tgcgccagct
     2401 tgtcgcaaag attaagaacg cgcaccagat cctgtgatgg ctggacatca ccaccagtac
     2461 cttcaacctg cggtaaacga tttaatgcct gattgatatc tgtgcgcaac tggccagcat
     2521 ttatgccagc ggatgttaat aaaggactaa ccgaaccccc ttcctgattc agcagggcgc
     2581 tcattaaatg aagtggttcg ataaattggt tgtcgtgccc aagtgcaagt gattgggcat
     2641 cggcaagagc aagctggaat ttattagtaa gacgatccag acgcataact cctcccataa
     2701 cggatcaaag ttgctactgg agattaaatg aggtcatccc tcaattattc aaggttattg
     2761 accagattaa tgtgaaaaga aaatcacgcg taccggatcg tcttgattct ttaggttata
     2821 tcagccaaat gaaacttgcc atacgaccgg tggtcttgtc gcgacgataa gagaagaaag
     2881 tctcattttc cgtatatgta caacggtcgc cgccgaaaat ttgctcaaca cccacgttcg
     2941 ccagacgctg ccgggcaagc tgataaatgt ccgccagata cttatcacca tgctgaatga
     3001 aagctgtact cgcttcggcg tctactgcca taaacgcctc gcgaacctcc gcccccactt
     3061 caaacgcgcg tgggccaatt gccggcccta accaggcgag aatattttct ggattatcag
     3121 caaaacagga aactgtctct tccagcacgc ctgcgcacag tccacgccag ccagcatgag
     3181 cggcggcgac ttccgttccc gctcgattgc aaaacagcac agggaggcag tcggcagtca
     3241 tcactgcgca aaccctgccg ggcgtattgc tataagaggc atctgctcgt tttgaggcat
     3301 aaggttcgcc agtaagatta agcacatctt tgccgtgtac ctgctcaagc cagaccggtt
     3361 tagaaggcaa attgcccgca gcaaaaagtc gcttgcgatt ctcctcaacg tgatccgggt
     3421 tatcgccaca atgggcaccg aggttgagcg agtcatacgg gggcaagctc acgccgccga
     3481 tacgagtaga gctacaggcc gcaacacctt ttggcagcgg ccactgcggg acaatcagct
     3541 tactcataac cagtccactt catctttatg ctcttcgaaa tcggcgcgca tcacctcaat
     3601 cagctccacc atatcttgtg gaataggcgc atgccattcc atttcgatac cggagatcgg
     3661 gtgataaaga cgcagcatgg ttgcatgaag cgcctggcgg tcaaatttac gcagcgtgga
     3721 gataaacgct tccgaagcac cttttggcgg gcgcggacgg ccaccataaa ccggatcgcc
     3781 caccagcgga tgagtgatat gggccatatg cacgcggatc tggtgcgtac gtccagtttc
     3841 cagacgcaac cgcagacgcg tgtgcacacg gaagtgttcc atgatgcgat agtgagtcac
     3901 cgccggtttg cccatcggat gcaccgccat gtgagtacgt ttggtcgggt ggcggctgat
     3961 tggttcatct actgtaccgc cagcggtcat atgaccaatt gccaccgctt catactcacg
     4021 agtaatttca cgcctttgca aagattcgac taaacgcgtc tgagccggaa cggtttttgc
     4081 cacaaccatc aggccagtgg tgtctttatc cagacgatgg acgatgcctg cacgcggtac
     4141 atcagcaatg ggcggatagt aatgaagtaa cgcattcagt accgtgccat ccgggttacc
     4201 cgcgccagga tgtaccacca gatcgcgcgg tttattaatg acgatgatat cttcatcttc
     4261 atagacgata tccagcggga tatcctgcgg ttcaaaacgc gcttcttctt caatctcagc
     4321 gttgatggca acctgctcgc cacccaatac tttttctttc ggcttatcac aaagtttgcc
     4381 gttaaccagc acgcgctggt cgaggatcca ttcttttatt cgcgaacgtg aataatccgg
     4441 gaacatttcg gccaaagcct gatctaagcg ttgaccgagt tggttttcgg acaccgttgc
     4501 agtgagctgt actcgttgtg ccatatatac tgcttcttcg tttatcgttg gggttttacg
     4561 gctttgccgt ttaatatagt gtgctattgt agctggtctt aaccgggagc aggaacagag
     4621 aatctcccgt attacatttt gaggaaagtc aaaacgtcat gacgcgcatg aaatatctgg
     4681 tggcagccgc cacactaagc ctgtttttgg cgggttgctc ggggtcaaag gaagaagtac
     4741 ctgataatcc gccaaatgaa atttacgcga ctgcacaaca aaagctgcag gacggtaact
     4801 ggagacaggc aataacgcaa ctggaagcgt tagataatcg ctatccgttt ggtccgtatt
     4861 cgcagcaggt gcagctggat ctcatctacg cctactataa aaacgccgat ttgccgttag
     4921 cgcaggctgc catcgatcgt tttattcgcc ttaacccgac ccatccgaat atcgattatg
     4981 tcatgtacat gcgtggcctg accaatatgg cgctggatga cagtgcgctg caagggttct
     5041 ttggcgttga ccgtagcgat cgcgatcctc aacatgcacg agctgcgttt agtgactttt
     5101 ccaaactggt gcgcggctat ccaaacagtc agtacaccac cgatgccacc aaacgtctgg
     5161 tattcctgaa agatcgtctg gcgaaatatg aatactccgt ggccgagtac tatacagaac
     5221 gtggcgcatg ggttgccgtc gttaaccgcg tagaaggcat gttgcgcgac tacccggata
     5281 cccaggctac gcgtgatgcg ctgccgctga tggaaaatgc ataccgtcag atgcagatga
     5341 atgcgcaagc tgaaaaagta gcgaaaatca tcgccgcaaa cagcagcaat acataacaga
     5401 aacctgaaac acaaaacggc agcccttgag ctgccgtttg tttattctgt cagttgtgaa
     5461 actgaagcga tttagtcact atcgatctca tcaaatatgg ctcgctttga gatattcctc
     5521 aagtaaaaaa acacctcttc ctgcgatttc tcacaaaaaa gattcgttga caaaaagtga
     5581 caaaattatg agatttccat cacacatttt gacatcagga acggtatgct gaattcacca
     5641 agacgggaag acaagaggta aaatttatga caatgaacat taccagcaaa caaatggaaa
     5701 ttactccggc catccgccaa catgtcgcag accgtctcgc caaactggaa aaatggcaaa
     5761 cacatctgat taatccacat atcattctgt ccaaagagcc acaagggttt gttgctgacg
     5821 ccacaatcaa tacacctaac ggcgttctgg ttgccagtgg taaacatgaa gatatgtaca
     5881 ccgcaattaa cgaattgatc aacaagctgg aacggcagct caataaactg cagcacaaag
     5941 gcgaagcacg tcgtgccgca acatcggtga aagacgccaa cttcgtcgaa gaagttgaag
     6001 aagagtagtc ctttatattg agtgtatcgc caacgcgcct tcgggcgcgt tttttgttga
     6061 cagcgtgaaa acagtacggg tactgtacta aagtcactta aggaaacaaa catgaaacac
     6121 acaccgtttt tcttcgcatt cttttttacc ttcccctgaa tgggaggcgt ttcgtcgtgt
     6181 gaaacagaat gcgaagaaga acaataaggc ctcccaaatc ggggggcctt ttttattgat
     6241 aacaaaaagg caacactatg acatcggaaa acccgttact ggcgctgcga gagaaaatca
     6301 gcgcgctgga tgaaaaatta ttagcattac tggcagagcg gcgcgaactg gccgtcgagg
     6361 tgggaaaagc caaactgctc tcgcatcgcc cggtacgtga tattgatcgt gaacgcgatt
     6421 tactggaaag attaattacg ctcggtaaag cgcaccatct ggacgcccat tacattactc
     6481 gcctgttcca gctcatcatt gaagattccg tattaactca gcaggctttg ctccaacaac
     6541 atctcaataa aattaatccg cactcagcac gcatcgcttt tctcggcccc aaaggttctt
     6601 attcccatct tgcggcgcgc cagtatgctg cccgtcactt tgagcaattc attgaaagtg
     6661 gctgcgccaa atttgccgat atttttaatc aggtggaaac cggccaggcc gactatgccg
     6721 tcgtaccgat tgaaaatacc agctccggtg ccataaatga cgtttacgat ctgctgcaac
     6781 ataccagctt gtcgattgtt ggcgagatga cgttaactat cgaccattgt ttgttagtct
     6841 ccggcacgac tgatttatcc accatcaata cggtctacag ccatccgcag ccattccagc
     6901 aatgcagcaa attccttaat cgttatccgc actggaagat tgaatatacc gaaagtacgt
     6961 ctgcggcaat ggaaaaggtt gcacaggcaa aatcaccgca tgttgctgcg ttaggaagcg
     7021 aagctggcgg cactttgtac ggtttgcagg tactggagcg gattgaagcg aatcagcgac
     7081 aaaacttcac ccgatttgtg gtgttggcgc gtaaagccat taacgtgtct gatcaggttc
     7141 cggcgaaaac gacgttgtta atggcgaccg ggcaacaagc cggtgcgctg gttgaagcgt
     7201 tgctggtact gcgcaaccac aatctgatta tgacccgtct ggaatcacgc ccgattcacg
     7261 gtaatccatg ggaagagatg ttttatctgg atattcaggc caatcttgaa tcagcggaaa
     7321 tgcaaaaagc attgaaagag ttaggggaaa tcacccgttc aatgaaggta ttgggctgtt
     7381 accctagtga gaacgtagtg cctgttgatc caacctgatg aaaaggtgcc ggatgatgtg
     7441 aatcatccgg cactggatta ttactggcga ttgtcattcg cctgacgcaa taacacgcgg
     7501 ctttcactct gaaaacgctg tgcgtaatcg ccgaaccagt gctccacctt gcggaaactg
     7561 tcaataaacg cctgcttatc gccctgctcc agcaactcaa tcgcctcgcc gaaacgctta
     7621 tagtaacgtt tgattaacgc cagattacgc tctgacgaca taataatgtc ggcataaagc
     7681 tgcggatcct gagcgaacag tcgcccgacc atcgccagct caaggcggta aatcggcgaa
     7741 gagagcgcca gaagttgctc aagctgaaca ttttcttctg ccaggtgcag cccgtaagca
     7801 aaagtagcaa agtggcgcag tgcctgaata aacgccatat tctgatcgtg ctcgacggcg
     7861 ctaatacgat gcaaccgagc gccccagacc tgaatttgct ccagaaacca ttggtatgct
     7921 tccggtttac gtccatcaca ccagaccaca acttgctttg ccaggctacc gctgtccgga
     7981 ccgaacattg ggtgtaaccc cagtaccggg ccatcgtgcg ccgccagcat ggcctgtaat
     8041 ggtccatttt tcactgatgc cagatcaacc agaatacaat ctttcggtaa aggcggtaat
     8101 ttgccaataa cttgctcagt aacgtggatt ggcacactaa caatcaccat tccggcatcg
     8161 gcaacaatat cagccgctcg atcccagtca tgttgctcca gaatccgcac ctgataaccc
     8221 gagagtgtca gcatcttctc gaacagacgt cccatctgac cgccgccgcc aacgataact
     8281 accgggcgta acgcaggaca aagcgtttta aatcctttgt cgttttcact ggagtaagat
     8341 tcacgcatca cccgacgcaa aacatcctca atcagatctg gcggtacacc cagagcttcc
     8401 gcctctgcac gacgcgaggc caacatagat gcctctcgct ccggaacata aataggcagt
     8461 ccaaagcggc ttttcacctc gcccacttca gcaaccagtt ccagacgctt cgctaataaa
     8521 ttcagcagcg ctttatcgac ttcatcaatt tgatcgcgta atgcggtcaa ttcagcaacc
     8581 ataataaacc tcttaagcca cgcgagccgt cagctgcccg ttcagatcct gatgaatttc
     8641 acgcagcaag gcatcggtca tttcccagct aatgcaggca tcggttacgg atacaccgta
     8701 tttcatttca ctgcgcggtt gctcggaaga ctgattgccc tcgtggatat tactttcgat
     8761 catcagacca ataattgagc gattgccatc tttgatttga gcaaccacgg attctgccac
     8821 cgcaggctga cggcgataat ctttattgga attaccgtgg ctgcaatcta ccatcagaga
     8881 cgggcgcagt cccgcctgtt ccatctcttt ttcacattgc gcaacatccg cagggctata
     8941 gttcggcgct ttaccaccgc gcaggatgac atgcccgtcc ggattcccct gagtttgtag
     9001 caacgcaacc tgccctgcct ggttaatgcc aacaaaacgg tgcggctggg cggcggcgcg
     9061 catagcgtta attgctgttg ccagactgcc gtcggtgccg tttttaaagc caaccggcat
     9121 ggaaagcccg gaggccattt cacggtgagt ttgcgattcc gttgtacgag caccaattgc
     9181 tgaccagcta aacagatcgc ccaggtattg cgggctattc ggatctaacg cttccgtcgc
     9241 cagtggcagt cccatattca ccagctcaag cagcaattta cgcgcgatct gcagcccggc
     9301 ttctacatca aaagagccat ccatatgggg atcgttaatt aaccctttcc agccgacagt
     9361 ggtacggggt ttttcaaaat agacgcgcat taccagatag aggctatcgc tgacctctgc
     9421 ggcaagggct ttaaatcgac gagcatattc cagagcagtt tccggatcat gaatggaaca
     9481 agggccacat actaccagca gacgaggatc gcgcccggcg ataatatctg aaatggtttt
     9541 acgcgagtca gcaatctggg cttcttgttg caggcttaat ggaaaagcgg ccttcagttg
     9601 tgccggagtc attaaaacct gttcgtcggt aatatgtacg ttattcagcg cgtctttttg
     9661 catgatggcg atcctgttta tgctcgtttg cgatagttga tcctcagcga ggatgacgta
     9721 acgataacac ataaagtaaa gttttcaatc catatttcgt acatttttat ttacataagc
     9781 aatttattcg cgctttcaac ccttgattct gtatagataa atttacacca cctttgaaaa
     9841 caatccgcta tgctttgaaa aaggagaaag aaatgatgaa aaagtttatc gcccccttgt
     9901 tggctttact ggttagcgga tgccagattg atccttatac tcacgcgcca accttgacca
     9961 gcaccgactg gtatgatgtt ggtatggaag atgcgatatc gggcagcgcc ataaaagatg
    10021 acgatgcatt tagcgattca caggcggatc gcggtctata ccttaaagga tatgccgaag
    10081 gacaaaagaa aacttgccag accgatttta cttatgcccg aggactttcc ggtaaaagct
    10141 ttcctgccag ctgcaacaat gttgaaaatg ccagccaact gcatgaagta tggcaaaaag
    10201 gggctgatga aaatgccagc gcaatacgat taaattaatt aaaaccccac aaataaatta
    10261 aacataagat tttactcatt atgttttatt tctcacaatg atgacgaccg catgaaataa
    10321 tgatatttta atgagcattc tgaaaaaata tcgatgcatt tcgagcgaag atggtgggga
    10381 tccctgaatg cgtttttctc accgactgtt cttactgctg attctcctgc tgacgggagc
    10441 accaatactc gctcaggagc cttctgacgt tgcgaaaaac gtgcgtatga tggtttccgg
    10501 tattgtcagt tacacccgct ggcctgcgct atcagggcca ccaaagttat gtatcttttc
    10561 atcttcacgt tttagtaccg ccctacaaga gaatgccgca acatctttgc cttatctgcc
    10621 tgtcatcatt cacactcaac aagaggcgat gatttcaggc tgcaatggtt tttattttgg
    10681 caacgagtca ccgacattcc agatggaatt aaccgaacaa tatccgtcaa aggcgttatt
    10741 attaattgcc gaacaaaata ccgagtgcat tattggtagc gccttttgtc tgatcatcca
    10801 caataatgac gtcagatttg ccgtaaacct ggatgcctta tcgcgtagcg gtgtaaaggt
    10861 caacccggat gtcttaatgc tcgcacggaa gaaaaatgat ggataacgat aattctctta
    10921 ataagcgccc cacgtttaaa agagcattac gcaacatcag tatgaccagc atatttatca
    10981 ctatgatgct gatctggttg ctgctttccg tgacctcggt gctgcccctg aaacagtacg
    11041 cgcaaaaaaa cctggcactg acagcagcaa caatgactta cagtctggaa gcagctgtcg
    11101 tttttgccga tggccctgca gcaactgaaa cactggcagc gctgggccag caagggcaat
    11161 tttcaactgc agaagtacgt gataagcagc aaaatattct ggcatcctgg cattacaccc
    11221 gtaaggatcc aggcgatact ttcagcaatt tcataagcca ctggctcttc cctgccccca
    11281 tcattcagcc gattcgtcac aatggtgaaa ccattggcga agtacgctta accgctcgcg
    11341 acagttcaat cagccatttt atctggtttt cgctcgccgt actgaccggt tgtattctgc
    11401 tggcatcagg aatcgcaatt accctcaccc gccatttgca caatggcctg gtggaagcac
    11461 tgaaaaatat caccgatgtc gtacatgatg tgcgttccaa ccgcaatttt tcccgacgag
    11521 tttcggaaga acgtatcgct gagtttcacc gcttcgctct cgacttcaac agtctgctgg
    11581 atgaaatgga agagtggcag cttcgtttac aggccaaaaa tgcgcagctt ctacgtaccg
    11641 cgctacatga cccattaacc gggctggcta accgcgcagc gtttcgtagc ggcatcaata
    11701 cgttgatgaa caattccgct gcccgaaaaa cgtcggcgtt actatttctt gatggcgata
    11761 atttcaaata tatcaatgat acctggggtc atgcgacggg cgatagagtc ttgattgaaa
    11821 tcgcaaaacg gttagctgaa tttggcgggc tgcgacataa agcataccgc ctgggcggcg
    11881 atgaattcgc tatggtgctc tatggtgtac agtcggaatc tgaagtgcag cagatatgct
    11941 cagcactgac acaaatcttt aatctcccgt ttgatcttca taatggccat cagaccacca
    12001 tgacattaag cattggttac gcaatgacca ttgcgcacgc ttctgcggaa aaattacaag
    12061 agctggccga tcacaatatg tatcaggcca aacaccagcg tgccgaaaag ctggtgagat
    12121 aacaaggata tatcgatatg ataaagcacc tggtagcacc cctgattttc acctcactaa
    12181 tactgactgg ctgccagtcc cctcagggaa agtttactcc tgagcaagtc gccgctatgc
    12241 aatcttatgg atttactgaa tccgccggcg actggtcgct gggcttatca gatgccattc
    12301 tgttcgcaaa aaatgactac aaattgctcc cggaaagcca gcaacagatc caaaccatgg
    12361 cagccaaatt ggcctcgaca gggctaacac atgcccgtat ggatggacac accgataact
    12421 atggtgaaga cagttacaac gaaggcttat cattgaaacg ggcgaatgtc gtggccgatg
    12481 catgggctat gggtggacaa attccacgca gcaatctcac cacacagggt ttaggaaaaa
    12541 aatatcccat agccagtaac aagaccgccc agggccgcgc cgagaaccgc cgcgtcgcag
    12601 tggtgattac taccccttaa aacacgagca aacagataaa aaaagggcca gccaattggc
    12661 cagcccttct taacaggatg tcgcttaagc gaaatcttag ttaagacgct ctttgatacg
    12721 agcagcctta ccagtacgct cacgcaggta gtacagttta gctttacgaa cagcaccacg
    12781 acgtttgaca gaaatgctgt caactaccgg agagtgagtc tggaagacac gctcaacgcc
    12841 ttcgccgttg gaaattttac gaacagtgaa tgcagagtgc agaccgcggt tacgaatagc
    12901 gataaccacg ccctcgaatg cctgcagacg ttttttggaa ccttcaacaa cccatacttt
    12961 cacttccacg gtatcacccg gacggaagga aggtacgtcc tgcttcatct gctcttgttc
    13021 aagttgctta ataatgttgc tcataattta atctcttatc ctgggtaaac tgatatctcg
    13081 ggggcttacg ccatcccatc atgtttatgt tgctgttgtg cgtgttccgt tttgaactcc
    13141 gccagcaacc ttgcttgctc ttcagtcaga gccaggtttt ccagaagttc aggtcttcta
    13201 agccaggtac ggcccagcga ctgtttcaaa cgccagcgac gtatctcggc atggttgccc
    13261 gacagtaaca ctggcggaac ttccatccct tctaacacct caggccgcgt atagtgcggg
    13321 caatccagca atccttcagc aaaggaatct tccgttgccg aggcttcatg tcccagtact
    13381 cccggaataa accgggaaac ggagtcaatc agcgtcattg ctggtaactc accaccactg
    13441 agaacgtaat cgccgattga ccattcttcg tcaatttcgg tttggatcac gcgctcatct
    13501 ataccttcgt agcgaccgca caccagaatc aatttttgat tcgttgccag ttcgctgacg
    13561 cccgcttgat caagcttgcg tccctgtggt gaaagataaa tcacctttgc gccttcaccc
    13621 gccgcggctt ttgctgcatg aatggcgtcc cgcaagggtt gcaccatcat taacatcccc
    13681 ggtccgccgc cgtaaggacg atcgtccacg gtacggtgcc ggtcatgcgt gaaatcgcga
    13741 ggactccagc tctggatgct cagcaggcca tttttaactg cccggccagt taccccgtaa
    13801 tcggtaattg cgcggaacat ttcaggaaac aggctaatta tgccaatcca catagcgccg
    13861 tcttttaccg tttatccggt ggtttaaaaa ccaggatccc aatctacttc gattgaacga
    13921 gtagtgagat cgactttctt gataacctgc ccatcgagga acggtacgag acgttccttg
    13981 ataccaaacg catctttcag gtttgcctta atgacgagaa cgtcattgga tccggtttcc
    14041 atcatatcga cgactttacc gagatcgtag ccttcagtgg ttactacctg gcagcccatc
    14101 aggtctttcc agtagtagtc accctcttca agctgaggca gctgcgatga atccacgaca
    14161 atttcacaat tcgtcagcag gttcgccgca tcacgatcgt caacgccttt cagcttgatg
    14221 atcatgtcct gattgtggtg cttccagctt tccagctgga cttgctgcca ctgacccgcc
    14281 ttctggataa accagggctg atagtcaaaa atgctttcgg cgtcttcggt ggaagaaaac
    14341 actctgagcc acccacgaat accgtaagac gaacccattt ttcccaaaac gatgggatca
    14401 acaggtgctt gcgcggtgag ttgtttgctc atcatgacca ccgtgacaga ttaagctgct
    14461 ttgtttactt ctttgatcag cgcagcaacg cgatcagaaa tagttgcgcc ctggccaacc
    14521 cagtgagcga tgcgatccag atccaggcga gtgccttctt ctttttcgct agcgattggg
    14581 ttgaagaaac caacgcgctc gatgaagcga ccgttgcgtg cattacggct gtcagcgaca
    14641 acaacctggt agaacggacg ctttttagcg ccgtgacgtg ctaaacgaat agttaccata
    14701 acatcctctt gtgtgaataa aacaaccgga ccccatcgag gaacggagtc cggtgtcata
    14761 ttaaaagccc gaaaatttta ctgatttctg gagaaaatgc aatcagcagt taataactct
    14821 gtcgcataag gtcatttagc gaccaggaaa tcccggcggc atcatcccct tcatgcttct
    14881 catcatcttc gccattccgc ccttcttcat tttcttcatc atgcgctgca tgtcgtcgaa
    14941 ctgtttcaga agacggttaa cgtcctgcac ctgcataccg caaccggcag caatacggcg
    15001 tttacgcgaa cctttgatga tttctggctt agcgcgctct ttcatcgtca tcgagttgat
    15061 gatggcttcc atacgcacca gcactttatc gtccatctgt gacttgacgt tatccgggat
    15121 ctgccccatg cccggcagtt tgcccatcag gctggccatg ccgcccatat ttttcatctg
    15181 gcgcagttgc tcaaggaagt cgttgagatc gaagccgtca ccttttttca gcttgctggc
    15241 taatttctct gcctgcgcgc ggtcaacttt gctttcgata tcttcgatca gcgacagtac
    15301 gtcgcccatg ccgagaatac gcgacgcgat gcggtccgga tggaacggct ccagcgcctc
    15361 agttttctcg ccaacaccga ggaacttgat cggtttgcca gtgatgtgac gaatagagag
    15421 cgccgcaccg ccgcgggcat cgccgtccac tttggtcaac actacgccgg taagcggtaa
    15481 cgcttcattg aatgcttttg ccgtattggc cgcatcctga ccggtcatgg cgtcaaccac
    15541 aaacagggtt tcaaccgggt taatcgacgc atggacttgt ttgatctcgt ccatcatcgc
    15601 ttcgtcaacg tgcagacgac cagcggtatc caccagcagc acgtcgtaga atttcagttt
    15661 ggcttctttc agcgccgcgt taacaatatc aaccggcttc tgaccaacat cagaagggaa
    15721 gaaatcaacg cccacctgct ctgccagcgt ctcaagctgt ttgattgccg ccgggcgata
    15781 gacgtcagca gaaaccacca gcactttctt cttgtgcttc tcgcgcagga acttaccgag
    15841 tttaccaacg ctggttgttt taccggcacc ttgcaggcct gccatcagca cgaccgcagg
    15901 cggttgcgca gccagattca gggtctggtt ctcttcgccc atcgccgcaa ccagttcgtt
    15961 acggacgatt ttgacgaact cctgccccgg cgtcaggctc ttattaactt catgaccaac
    16021 cgctttctct tttacgcgat tgataaactc acgcactacc ggcagagcga cgtccgcctc
    16081 cagcagcgcc atgcgcactt cgcgcagcgt atcttttacg ttgtcttcag tgaggcgtcc
    16141 acggccactg atattgcgca gcgtgcgcga caaacgatcg gttaaattat caaacattgt
    16201 ctctcgcctg gggtggaaac ggttggccgc aatcgcgaca catcatcagt attttgccgc
    16261 agtataacat gaaggcgtct ttgttgttat gcaacggttg gagcagcgtt cacctgacgc
    16321 tatactgctt ctctttctta ttgctcaaac tgtcgacatc actatgcccg tttttgctct
    16381 gctcgcgctt gtcgcctact ccgtcagtct tgcgctgatt gttcccggtc tgctgcaaaa
    16441 aaacggcggc tggcggcgca tggctattat ttctgcggtc attgcgctgg tctgccacgc
    16501 aatcgctctg gaagcccgca tcctgcccga cggtgatagc ggacaaaacc tcagcctgct
    16561 gaacgttggt tcattggtca gtttgatgat ctgtacggta atgaccattg tggcttctcg
    16621 caatcgtggc tggttgctgc tacccattgt ctatgccttt gcgcttatca acctggcgct
    16681 ggcaaccttc atgcccaatg aatacatcac ccatctggaa gctacgcctg ggatgctggt
    16741 gcacattggc ttatcgctct tttcctatgc cacgctaatt atcgccgccc tgtacgcgct
    16801 gcaactggcg tggattgatt accaactgaa gaacaagaag ctggcgttta accaggaaat
    16861 gccgccattg atgagtatcg agcgtaaaat gttccacatc acgcagattg gcgtggtgct
    16921 gctaacgctc acgctttgca ctggcctgtt ctacatgcac aacttattta gcatggaaaa
    16981 tatcgacaag gctgtgctct ctatcgtggc gtggtttgtc tatattgtgc tgctatgggg
    17041 acattatcat gaaggatggc gtggacgtcg cgtcgtctgg tttaacgttg cgggtgcagt
    17101 cattctgaca ctggcctact tcggcagccg aattgtccag cagttaatca gctaaaccca
    17161 gaaaaggagt ttccccctgg aacacatttc tactactacg ttgatcatta ttctgatcat
    17221 catggtggtc atttcagcct atttttccgg gtccgaaacc ggaatgatga ccctcaaccg
    17281 ctatcgtctg cgacatatgg cgaaacaggg taatcgctcg gccaaacgcg tcgaaaaatt
    17341 gctgcgtaag ccagaccgcc tgataagcct ggtgttaatc ggcaataacc tggtcaatat
    17401 tcttgcctcc gcgctcggca ctattgttgg gatgcgtttg tacggcgatg cgggcgtggc
    17461 aattgcgact ggtgtgctga cttttgtcgt actggttttt gctgaggtat tgccgaaaac
    17521 cattgccgcg ctgtacccgg aaaaagtcgc ttatccgagt agttttctgc tggctcccct
    17581 gcaaattttg atgatgccgc tggtctggtt gctgaatgct atcacccgta tgctgatgcg
    17641 catgatgggt atcaaaaccg atatcgtggt tagcggctct ttgagcaaag aagagttgcg
    17701 cactatcgtg cacgaatcgc gctcacaaat ttcccgtcgc aatcaggata tgctgctgtc
    17761 ggtgctcgat ctggaaaaaa tgaccgttga tgacatcatg gtgccgcgca gtgaaattat
    17821 cggtattgat atcaacgatg actggaaatc tattctgcgc caactctccc actcacctca
    17881 cgggcgcatc gtgctctacc gtgattcgct ggacgacgcc atcagtatgc tgcgagtacg
    17941 tgaagcttgg cggttgatgt cggagaaaaa agagttcacc aaagaaacca tgctgcgcgc
    18001 cgcggacgag atctattttg tgccggaagg tacgccgctc agcacgcagt tggtgaagtt
    18061 tcagcgcaac aaaaagaaag tcggcctggt cgtcaacgag tatggagaca ttcaggggct
    18121 ggtgacggtt gaagatattc tggaagagat tgtcggcgat ttcactacgt cgatgtcgcc
    18181 aacacttgcc gaagaagtca cgccgcaaaa cgacggttcg gtgattattg atggcactgc
    18241 caacgtgcgg gaaatcaaca aagcctttaa ctggcatcta ccggaagatg atgcccgcac
    18301 ggttaatggc gtcattcttg aggcactgga agagatccct gtcgtaggca cccgcgtgcg
    18361 tattggcgag tacgatatcg atattctcga cgtacaggac aatatgatta agcaggtaaa
    18421 agtttttcct gtgaaaccgc tgcgcgagag tgtggcggag taacgaaaac ggcccggcat
    18481 tcgcatgcag ggccgtgaat tattacgaaa acagaaatta cgcttttgct ttcgctacag
    18541 ttaccatcgc cgcacgaatc gtacgaccat tcagcgtata acccttctgc ataatgccca
    18601 gtacgttacc tggcgcaacg tcatcagatt ccaccattgc gatagcctga tgcacattcg
    18661 ggtccagcgg cacgttagtt tcggcgatca cttcaacacc aaacttacgc acaacatcca
    18721 gcatcgactt cagcgtcagc tcaatgcctt caaccatcgc agacatatcc gggttagctt
    18781 tatcagccac ttccagcgca cgatccaggc tatcaatcac cggcagcaat tcgttgatga
    18841 atttctccag cgcgaatttg tgggcttttt caatatccag ttcagtacga cgacgcaggt
    18901 tttccatttc ggcttttaca cgcaaaatgc cgtcacgttc acgggtctgg gcttcagcca
    18961 gctgagcttc gagattcgca attttttcat cgcgcggatc cacctgctca gcagaagctt
    19021 ctggctcaac tgcctcaatc tcttcgtgct gatccatgat aatttcttcc ggggcttgcc
    19081 cctcaggcgt tttctgttct ttactactca tgaatttctc cgcgtttttt tcgcattcat
    19141 ctcgctaact tcgcttatta tggggatcag tttcagggtt tcaagggaag cactcacatt
    19201 gtcatcaatc ttcgcaacaa ggacctcgga aaaatgaata atcatttcaa gtgtattggc
    19261 attgtgggac acccacggca ccctactgca ctgacaacac atgaaatgct ctaccgctgg
    19321 ctgtgcacaa aaggttacga ggtcatcgtt gagcaacaaa tcgctcacga actgcaactg
    19381 aagaatgtga aaactggcac gctcgcggag attgggcaac tggctgatct cgcggtagtc
    19441 gttggtggcg acggtaatat gctgggcgcg acgcgcacgc tcgcccgtta cgatattaaa
    19501 gttattggaa tcaaccgtgg caacctgggt ttcctgactg accttgaccc cgataacgcc
    19561 cagcaacaat tagccgatgt gctggaaggt cactacatca gcgagaaacg ttttttgctg
    19621 gaagcgcaag tctgtcagca agattgccaa aagcgcatca gcaccgcgat aaatgaagtg
    19681 gtacttcatc caggcaaagt ggcgcatatg atagagttcg aagtgtatat cgacgagatc
    19741 tttgcgtttt ctcagcgatc tgatgggcta attatttcga cgccaacagg ctccaccgcc
    19801 tattccctct ctgcgggcgg tcctattctg accccctctc tggatgcgat taccctggtg
    19861 cccatgttcc cgcatacgtt gtcagcacga ccactggtca taaacagcag cagcacgatc
    19921 cgtctgcgtt tttcgcatcg ccgtaacgac ctggaaatca gttgcgacag ccagatagca
    19981 ctaccgattc aggaaggtga agatgtcctg attcgtcgct gtgattacca tctgaatctg
    20041 attcatccga aagattacag ttatttcaac acattaagca ccaagctcgg ctggtcaaaa
    20101 aaattattct aattttacgc cagcctcttt actgtatata aaaccagttt atactgtaca
    20161 caataacagt aatggttttt catacaggaa aacgactatg ttggcacaac tgaccatcag
    20221 caactttgct atcgttcgtg agcttgagat tgattttcat agcggcatga ccgtaataac
    20281 tggcgaaacc ggtgcgggta aatctattgc aatagatgcc ctcggtcttt gtctcggtgg
    20341 tcgcgctgaa gccgacatgg tgcgtaccgg cgctgctcgc gctgacctgt gcgcccgttt
    20401 ttctctgaaa gatacgccag cggccctgcg ctggctggaa gaaaaccagc ttgaagacgg
    20461 gcatgaatgt ttgcttcgtc gcgtgatcag cagcgatggt cgctcccgtg gtttcatcaa
    20521 cggtacagct gttcctctgt cacaactgcg cgaactgggt cagttgctga ttcagatcca
    20581 tggtcagcac gctcatcagt tactcaccaa acccgagcac caaaaattcc tgcttgatgg
    20641 ctatgccaat gaaacctctc tactccagga aatgaccgca cattatcagt tgtggcatca
    20701 aagctgccgt gacctcgcgc atcatcaaca gttaagtcag gaacgcgctg cccgtgcgga
    20761 actgctgcaa taccaattaa aagaacttaa cgaatttaat ccgcagcccg gagagtttga
    20821 gcaaatcgac gaagagtaca aacgtctggc gaacagcggt caattgctga ccaccagcca
    20881 gaatgcattg gcattaatgg ccgacggtga agacgcaaac ctgcaaagtc agctttacac
    20941 ggctaaacaa ctggtgagcg aattgattgg catggacagc aaactgtccg gcgtacttga
    21001 tatgctggaa gaagctacca tccagattgt tgaagccagc gacgaactgc gccactactg
    21061 cgatcgtctg gatctcgatc ccaaccgact gtttgaactt gaacagcgca tctcaaaaca
    21121 gatttcgctg gcacgtaaac atcacgtcag ccctgagaca ttgccacagt attaccagtc
    21181 gctactggaa gaacagcagc aactggacga tcaggccgac tcacaagaaa cgctcgcact
    21241 ggcggtaacg aaacatcatc agcaggcact ggaaacggcg cgcgcattac accagcaacg
    21301 ccagcattat gcagaagaac ttgcacagct gatcaccgac agtatgcatg cgctctcaat
    21361 gccgcatggg cagtttacga ttgatgtcaa atttgacgag catcacctgg gcgctgacgg
    21421 cgccgatcgt attgagtttc gggtaaccac caacccaggt cagccaatgc agcctattgc
    21481 caaagtcgca tccggtggtg aattgtcccg catcgcattg gcaattcagg tcatcacggc
    21541 gcgtaaaatg gaaaccccgg cactgatttt tgatgaagtg gatgtaggga ttagcggtcc
    21601 aacagcggca gttgtcggca aactgctgcg tcaactcggc gaatcaactc aggtgatgtg
    21661 tgttacccac ctgccacaag tcgcgggatg tggtcatcaa cactattttg tcagcaaaga
    21721 aaccgatggt gcgatgacag aaacgcatat gcaatccctg aataaaaaaa cgcggttaca
    21781 agagctggcg cgcctgcttg gtggcagtga agtcacacgt aacacactgg cgaatgcgaa
    21841 agaactgctt gcggcgtaaa cttttttcct gcttcacggt cagagtaaac agcaaaacgc
    21901 cgtaagaccg gaaagcaaaa ggttttaaag tgatgaaagg tctattatca tcggcatatt
    21961 acagatgagc cacgtactgc tcgggcccga aaaggaatca aatcactatg cgctgtaaaa
    22021 cgctgactgc tgcagcagca gtactattga tgttgaccgc aggctgttcc actctggagc
    22081 gagtggttta ccgtcctgac atcaaccagg ggaactatct gaccgctaac gacgtatcca
    22141 aaatacgtgt tggcatgacg caacaacaag ttgcgtacgc attgggtaca ccgctgatgt
    22201 ccgatccatt tggtacgaat acctggttct atgtcttccg ccagcaacca ggtcatgaag
    22261 gtgtaacgca gcaaacgctg acgctaacct ttaacagtag cggtgtgttg accaatattg
    22321 ataacaaacc tgcgctgagt ggtaactaat aataaagttg ctctcaaaga cgttaaaaaa
    22381 ggtgctcaat gagcaccttt tttctgtccg ttatttattc gctgattttt ctgctcgttg
    22441 cctgcgaagc tctttcggat cggcaatgag agggcgataa atctccaccc gatcgccatc
    22501 atgcacacta tcgcttaatt ttgccggacg gctgtaaatg ccgactttgt tttcagttaa
    22561 atcgatatcg gtacgcaatt ccagcaagcc actggcgcga atagcttctt caaccgtcgc
    22621 gccctcctgc agcgtcacgc gctgcaggta ctgcttctta ggtagcgcat aagccacctc
    22681 aacggcaatt ttacctggca ctgtaaacct ctttcgcacg aaccgtaaaa gcctggacca
    22741 tattagacgc cagctcttta aacacgcgac caaaagcgag ttcaatcaac ttattggtaa
    22801 actcaaagtc gagatgaaac tcgatacgac acgcctcctg gctcagcggc gtaaacttcc
    22861 atccaccaat caatttcttg aacggcccat ccaccagatt cataagaata ctttggttac
    22921 tggtcaactg gttgcgggta gtaaacgttt tgctgatccc agccttagag acatctaccg
    22981 ccgcagtcat ctgcccagga gtggactcca gaatccgact tccggtacaa cccggcaaaa
    23041 actgaggata agactgaacg tcattcacta actgatacat ttgctccgcg ctgtagggta
    23101 ccagtgcggt ccggctaatc tgaggcataa caatttccat caacaaaaat ccaacaaata
    23161 atatcattta tcctgcagta aaaaaaacgc tatcccggcg ctgggtaaca tccggttcat
    23221 gctaagatag agccttgtcc cccgcaggat tgatatgggg tattttcgat ttcagattac
    23281 cgatgattca cgacgcttat gacgaagaaa aaagcacata aacctggttc agcgaccatc
    23341 gcgcttaaca agcgcgcccg tcacgaatac tttattgaag aagagttcga agcgggactt
    23401 gccctgcaag gctgggaagt taaatccctg cgcgcaggaa aagccaatat tagcgacagc
    23461 tatgtccttc tgcgcgacgg agaagcgttc ctgtttggcg caaacatcac gccaatggct
    23521 gtggcttcta cgcatgtggt gtgcgatcct acccgtaccc gcaagttact tctcaaccag
    23581 cgcgaacttg attcgttgta cggtcgcgtc aatcgagaag gctataccgt agtggcgctc
    23641 tccctgtact ggaaaaatgc ctggtgcaaa gtgaaaatcg gcgtcgccaa aggtaagaaa
    23701 cagcacgata aacgttcaga tatcaaagaa cgcgaatggc aggtggataa agcgcgtatc
    23761 atgaaaaacg cccaccgtta aacctgcact ccaattcttg acctgttcct caccgcgcct
    23821 ccctctccgg cggcgcgaat gaacatctta ttggctatca catccgacaa aaatgttgcc
    23881 atcccattgc ttaatcgaat aaaaatcagg ctacatgggt gctaaatctt taacgataac
    23941 gccattgagg ctggtcatag cgctcataaa tctggtatac ttgccgttac acattggggc
    24001 tgattctgga ttcgacggga tttgcgaaac ccaaggtgca tgccgagggg cggttggcct
    24061 cgtaaaaagc cgcaaaaaat agtcgcaaac gacgaaaact acgctttagc agcttaataa
    24121 cctgcttaga gccctctctc cctagcctcc gctcttagga cggggatcaa gagaggtcaa
    24181 acccaaaaga gatcgcgtgg aagccctgcc tggggttgaa gcgttaaaac ttaatcaggc
    24241 tagtttgtta gtggcgtgtc cgtccgcagc tggcaagcga atgtaaagac tgactaagca
    24301 tgtagtaccg aggacgtagg aatttcggac gcgggttcaa ctcccgccag ctccaccact
    24361 ttaagaagga ctacaaccgg acagtagcaa taaatacagc cacttacgga cacaagccag
    24421 acagtagtcg gactgaaaaa agacaaaaat atacacgtga aatgcacgtg cacttctaaa
    24481 aaaccccagt tatcaccgac tggggttttt caatttgtaa ctaagggtaa caaccccccc
    24541 gtttttttca tctcactcac cttgatactg tttatttgta cagtta
//
LOCUS       DAZFAB010000068        24549 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZFAB010000068 DAZFAB010000000
VERSION     DAZFAB010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.068"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..134
                     /locus_tag="ACHJWA_003062"
     CDS             <1..134
                     /locus_tag="ACHJWA_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector EspY1"
                     /protein_id="HFO4309612.1"
                     /translation="DGATHLYDEVIIINKITPKKIDTEEVATKQSTAEQITDNAIIE"
     gene            complement(161..339)
                     /locus_tag="ACHJWA_003063"
                     /pseudo
     CDS             complement(161..339)
                     /locus_tag="ACHJWA_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase DNA-binding-containing protein"
     gene            complement(552..1211)
                     /gene="rluA"
                     /locus_tag="ACHJWA_003064"
     CDS             complement(552..1211)
                     /gene="rluA"
                     /locus_tag="ACHJWA_003064"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFO4309613.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFRERVPKKQYVTRVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(1223..4129)
                     /gene="rapA"
                     /locus_tag="ACHJWA_003065"
     CDS             complement(1223..4129)
                     /gene="rapA"
                     /locus_tag="ACHJWA_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFO4309614.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            complement(4293..6644)
                     /gene="polB"
                     /locus_tag="ACHJWA_003066"
     CDS             complement(4293..6644)
                     /gene="polB"
                     /locus_tag="ACHJWA_003066"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFO4309615.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILRGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGAIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASALDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRIPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     SIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLVSSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEATKIG
                     RALVQHVNVWWAETLQKQQLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYFRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            complement(6719..7414)
                     /gene="araD"
                     /locus_tag="ACHJWA_003067"
     CDS             complement(6719..7414)
                     /gene="araD"
                     /locus_tag="ACHJWA_003067"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFO4309616.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSIMTADDMVVVSIETGEVVEGAKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            complement(7614..9116)
                     /gene="araA"
                     /locus_tag="ACHJWA_003068"
     CDS             complement(7614..9116)
                     /gene="araA"
                     /locus_tag="ACHJWA_003068"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFO4309617.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVKALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAAEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            complement(9127..10827)
                     /gene="araB"
                     /locus_tag="ACHJWA_003069"
     CDS             complement(9127..10827)
                     /gene="araB"
                     /locus_tag="ACHJWA_003069"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFO4309618.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCTTGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRSEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKEQIDASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            11166..12044
                     /gene="araC"
                     /locus_tag="ACHJWA_003070"
     CDS             11166..12044
                     /gene="araC"
                     /locus_tag="ACHJWA_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFO4309619.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            12130..12894
                     /gene="yabI"
                     /locus_tag="ACHJWA_003071"
     CDS             12130..12894
                     /gene="yabI"
                     /locus_tag="ACHJWA_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO4309620.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLVRHPLMPVYIDILRKVVGV"
     gene            complement(12981..13679)
                     /gene="thiQ"
                     /locus_tag="ACHJWA_003072"
     CDS             complement(12981..13679)
                     /gene="thiQ"
                     /locus_tag="ACHJWA_003072"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFO4309621.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGALTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAVQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGMTNELL
                     SGKASASALLGITG"
     gene            complement(13663..15273)
                     /gene="thiP"
                     /locus_tag="ACHJWA_003073"
     CDS             complement(13663..15273)
                     /gene="thiP"
                     /locus_tag="ACHJWA_003073"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFO4309622.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGDWS
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVTALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTMLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFSVIEKIPGRNVKTD"
     gene            complement(15249..16232)
                     /gene="thiB"
                     /locus_tag="ACHJWA_003074"
     CDS             complement(15249..16232)
                     /gene="thiB"
                     /locus_tag="ACHJWA_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFO4309623.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPVVKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDIFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEKLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     regulatory      complement(16263..16362)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            16604..17173
                     /gene="espY2"
                     /locus_tag="ACHJWA_003075"
     CDS             16604..17173
                     /gene="espY2"
                     /locus_tag="ACHJWA_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspY2"
                     /protein_id="HFO4309624.1"
                     /translation="MKVRNPEQISIPASNTTKDPGLTNSQIIRMANLVKKTENMNIFE
                     ELWETLRNLFQSDKHSQTAARQILKDAFYFQNSDDYSKYFTGAVDGKARDKLTHWLIK
                     FNELKEYAKDPENMAAKASLSPEGTLCVSFFIGDEAIFTLELQLKKSTRTGGIDLSNA
                     YFNGVVICGIDCLEVDLSNAETNNSRWYD"
     gene            complement(17402..19060)
                     /gene="sgrR"
                     /locus_tag="ACHJWA_003076"
     CDS             complement(17402..19060)
                     /gene="sgrR"
                     /locus_tag="ACHJWA_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFO4309625.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     ITSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQVDAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDPE"
     gene            19149..19280
                     /gene="sgrT"
                     /locus_tag="ACHJWA_003077"
     CDS             19149..19280
                     /gene="sgrT"
                     /locus_tag="ACHJWA_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFO4309626.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWEGSHSDY"
     gene            complement(19388..19993)
                     /gene="leuD"
                     /locus_tag="ACHJWA_003078"
     CDS             complement(19388..19993)
                     /gene="leuD"
                     /locus_tag="ACHJWA_003078"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFO4309627.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFSQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMR"
     gene            complement(20004..21404)
                     /gene="leuC"
                     /locus_tag="ACHJWA_003079"
     CDS             complement(20004..21404)
                     /gene="leuC"
                     /locus_tag="ACHJWA_003079"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFO4309628.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGILL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            complement(21407..22498)
                     /gene="leuB"
                     /locus_tag="ACHJWA_003080"
     CDS             complement(21407..22498)
                     /gene="leuB"
                     /locus_tag="ACHJWA_003080"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFO4309629.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     IELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            complement(22498..24069)
                     /gene="leuA"
                     /locus_tag="ACHJWA_003081"
     CDS             complement(22498..24069)
                     /gene="leuA"
                     /locus_tag="ACHJWA_003081"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFO4309630.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRATEVEKE
                     LQRKAQHNENNKETV"
     gene            complement(24162..24251)
                     /gene="leuL"
                     /locus_tag="ACHJWA_003082"
     CDS             complement(24162..24251)
                     /gene="leuL"
                     /locus_tag="ACHJWA_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFO4309631.1"
                     /translation="MTHIVRFIGLLLLLNASSLRGRRVSGIQH"
BASE COUNT         5561 a         6607 c         6616 g         5765 t
ORIGIN      
        1 tggacggtgc tacccaccta tatgatgagg taattattat taataaaatc acccccaaaa
       61 aaattgatac tgaagaagtt gctactaaac aaagtactgc tgaacaaatt actgacaacg
      121 caattattga atgagccaga ataagctaag gttgaagggg ctggaacgcc ccttcaacct
      181 tagcagtagc gtgggatgat ttcacaatta gaaagacctg catgatgagc tagagaagag
      241 gctagtgacg caaggcgtcg tgtacgacgc ggatcaccga gatgggcatc gccaaccaga
      301 ctgctaatta gcccatgaat aacaatcaga aaggaccata acagacccga taaaatgaaa
      361 tataagagac ggtcaacggg tgaagaaaaa gttcaaaaat tcgctgtgga gcaggaaggg
      421 aattaccgaa tggaaagcgt agccacacgc aacaactgaa agcagtttgg cagaaacaaa
      481 aaatccccgg actcggggat ttatgtacaa gaggcagccc ttaggatgag ggtataaacg
      541 tacaggaaag gttaaaaatc cgctggcgct ttaaacgtca tactattgcc atacgccgga
      601 tgggtaatcg tcaacatctc tgcatgtagc aacaaacgcg gtgccatcgc tctcgcttct
      661 ggtgatgcat aaaaacgatc gccgagaatc ggatgcccca gcgccagcat atgcacacgc
      721 aattgatgcg aacgcccggt aatcggtttt aacaccactc ttgccgtgtt atccgccgca
      781 tactccacca cttcatattc cgtctgcgca ggtttacccg tttcgtaaca gactttctgt
      841 ttcgggcggt ttggccagtc gcaaatcagc ggcagatcca ccagaccttc tgcgggggat
      901 ggatgccccc agacgcgggt cacatactgc tttttcggca cgcgctcgcg gaactggcgt
      961 tttaactccc gctccgcggc tttggtcagc gccactacaa tcacgccgct ggtagccata
     1021 tccagacgat gcaccgattc tgcctgcgga taatctcgct gaatgcgcgt catcacgctg
     1081 tctttgtgct cttccagacg acccggcaca gacaacaaac cgctcggctt gttgaccacc
     1141 ataatatggt catcctgata caggataacc aaccagggtt cctgcggtgg attgtagttt
     1201 tccatcccca ttttcggctc cgttactgat gcgttacaac gatcaaacgc agggcatcca
     1261 gacgccaacc tgcctgatcc aggctttcca ttacctgctg acggttgctc tcaatggcgg
     1321 tcagttcgtc gtcacgaatg ttcgggttca ctgcacgcag agcttccaga cgagacagct
     1381 cggcagacag tttttcgtcg gcttcgttac gtgctgcatc aatcaatgca cgggcagatt
     1441 tctcgatctg cgcttcaccc agttgaagga tagcgtgaac atcctgctgc acggcgttaa
     1501 ccagtttgct gccggtgtga cggttaaccg cgttaagctg gcggttaaag gtttcaaact
     1561 ctacctgcgc cgccaggttg ttgccgtttt tatccagcag catacgtacc ggcgtcggtg
     1621 gcaggaagcg gttgagctgc aactgcttcg gagcctgggc ttcaaccaca taaatcagtt
     1681 ccaccaacag cgtacctacc ggcaacgctt tgttttttaa cagtgaaatc gtgctgctac
     1741 cggtatcgcc agaaagaatc agatccagac cgttgcggat cagcggatgt tcccaggtaa
     1801 taaactgcgc atcttcacgc gccagcgcca cttcacgatc aaaggtgatg gtgatgccat
     1861 cttccgacag gccagggaag tccggcacca gcatatgatc ggacggcgtc agcacgatca
     1921 tgttgtcgcc gcgatcgtcc tgattgatac cgataatatc gaacaggttc atggcgaagg
     1981 cgatcaggtt ggtatcgtca tcctgctctt caatgctttc tgccagtgcc tgggcttttt
     2041 cgccaccgtt ggagtggatt tccagcaggc ggtcacgacc ctgttccagc tgtgctttca
     2101 gcgcttcatg ttgctcgcgg cagtttttga tcagatcgtc aaagccttcg gtttgatccg
     2161 gtgaagccag atagttaatc agatcgttgt atacgctatc gtaaatagtg cgtccggtcg
     2221 ggcaggtgtg ctcaaatgca tccagacctt cgtgatacca gcgcaccagc accgactgag
     2281 cggttttctc cagataaggc acatggatct gaatatcgtg cgcttggccg atacgatcca
     2341 gacgaccaat acgctgctcc agtagatccg ggttgaatgg caggtcaaac atcaccatgt
     2401 ggctggcgaa ctggaagtta cgtccttcag aaccgatttc tgagcacagc agtacctgtg
     2461 cgccggtgtc ttcttcggca aaccaggcgg cagcgcggtc acgttcgata atcgacatac
     2521 cttcgtggaa caccgcagcg cgaatacctt cacgttcgcg cagtacttgc tccagttgca
     2581 gcgcagtggc agctttagcg cagatcacca gcactttctg agagcgatgg ctggtcaggt
     2641 agcccatcag ccactcaacg cgcggatcga agttccacca ggtggcgtta tcaccttcaa
     2701 attcctgata aatacgctcc gggtagagca tatcgcgggc gcggtcttcc gcacttttac
     2761 gtgcgcccat aatgccggag actttaatag ccgtctgata ctgcgtcggt agcggcagct
     2821 taatggtgtg cagctcgcgt ttcgggaagc ctttcacacc gttacgggtg ttacggaaca
     2881 gcacgcggct ggtgccgtgg cgatccatca gcatcgaaac cagctcctga cgggcgctct
     2941 gggcatcttc gctgtcgctg tttgctgctt gcaacagcgg ctcgatatcc tgctcgccga
     3001 tcatctcgcc aagcatgttc agttcgtcat tgctcagttt gttacctgcc agcagcatgg
     3061 caacggcgtc cgctactgga cgataatttt tctgctcttc aacgaattgc gcgaaatcgt
     3121 ggaaacggtt cgggtccagc agacgcagac gggcgaagtg gctttccatt cccagctgtt
     3181 ccggggttgc ggtcagcagc agaacgcccg gcacgtgctc tgccagttgt tcaatagcct
     3241 gatattcgcg gctcggcgcg tcttcgctcc acaccaggtg atgcgcttca tcgaccacca
     3301 gcaggtccca ctcggcttca cagagatgtt ccagacgctg tttgctacgg cgggcaaaat
     3361 ccagcgagca aatcaccagc tgttcggtgt cgaacgggtt gtaagcatcg tgctgagctt
     3421 cggcataacg ctcatcatca aacagcgcaa agcgcaggtt gaaacggcgc agcatttcta
     3481 ccagccactg atgctgtaag gtttccggga cgataatcag cacacgttca gcagcgccag
     3541 agagcagttg ctgatgaagg atcatcccgg cttcaatggt tttccctaaa cccacttcgt
     3601 cagccagcag gacgcgcggc gcgtggcggc ggccaacatc atgagcgatg ttgagctggt
     3661 gcgggatcag gctggtacgc tgaccgcgca ggccgctgta cggcatacgg aactgttcgc
     3721 tggaatactt acgcgcgcga taacgcagcg caaagcggtc catacggtca atctgcccgg
     3781 caaacaggcg gtcctgcggt ttgctgaaca ccagtttgct atcaaggaaa acttcacgca
     3841 gggctacgcc ggactcttca gtatccaggc gagtaccgat ataggtcagc aagccatttt
     3901 cttcttttac ttcttcgact tgcatctgcc agccgtcatg gctggtaatg gtatcaccag
     3961 ggttgaacat cacgcgggtc acgggggaat cactgcgtgc gtacagacgg ttttcaccag
     4021 tagatgggaa aagtaaagtg acagttcgcg catccaccgc gacaacggtt ccaagtccca
     4081 attcgctttc tgtatcgctg atccagcgtt gaccaagtgt aaaaggcata tgtgttcggc
     4141 tctatatctt taattgcagg caataaccac ccgctaccgt gcttatgagg tagtggtgtt
     4201 attcaggtcc aggaatggaa agggcgctat ggtactggat ggcaaagcat tcgtcacgca
     4261 tcaaaatggt atctggcgaa ctcttttttt gctcaaaata gcccaagttg cccggtcata
     4321 agtgtagcaa aattatcctc aataaaaggg agtattccct ccgccacggg ttgtagctgg
     4381 cgggtcagat agtgttcgta atccagtggt gaacgttggt agtccagcgg ctccgggccg
     4441 ttggtggtcc atacgtactt aatggtgccg cggttctgat attgcaaggg gcgaccgcgc
     4501 ttttggtttt cttcatcggc aagacgagcg gcgcgtacat gaggcggaac attacgctga
     4561 tattcgctca gcggacggcg aaggcgttta cggtaaacca gccgcgcatc cagttcaccc
     4621 gccatcagtt tgtcgatggt ttcgcggaag tactcctgat atggctcgtt gcggaagata
     4681 cgcaggtaaa gctcctgctg aaactgctgg gccagcggcg tccagtccgt acgcacggtt
     4741 tccagccctt taaacaccat ccgctgtttg tcgccctcct gaatcagccc ggcataacgc
     4801 tttttactgc cggtatcggc tccgcgaatg gttggcatca gaaaacggca gaaatgggtt
     4861 tcatactcca gttccagtgc gctggtcagc tgttgttttt gcagcgtttc tgcccaccag
     4921 acgttaacgt gctgcaccag tgcacgaccg attttcgtcg cttcttcttc cgaatgtgcg
     4981 cctttcagcc agacaaacgt tgagtcggta tcgccgtaga tcacgtcgta gccctgcgct
     5041 tcaatcaacg ctttggtttg ccgcatgatc tgatgaccac gcatggtgat cgacgatacc
     5101 agccgcggat cgaagaagcg gcaggcggtg gtgccgagca cgccataaaa ggcattcatg
     5161 atgattttca gcgcctgcga cagcggtttg ttaccctggc gtttggcttc atcgcgtccg
     5221 tgccagatgt tagtcacaat ctccggcagg caatgttttt ctcgcgagaa ccaggcatcg
     5281 agaaagcctt cggtgctgtg ctctggatca ggctgcgcca tgccttccac cagcccgacg
     5341 ggatcaatca gaaaggtgcg gatgatcgac gggtacaggc ttttgtagtc cagcaccagc
     5401 accgaatcat aaagcccagg tcgcgaatcc atcacgtagc cgccagggct ggcgtgcggc
     5461 ggcacttcgc cgagattagg cgcgacataa ccagcgcgat gcatacgggg aaaatagaga
     5521 tgaccaaacg ctgccaccga cccgccgtgt cgatccaccg gcaggccgtt caccgttgct
     5581 cgttcgagca aaaacggcat gatttcagtt ttatggaaga tctgcgtcac cagctcgcaa
     5641 tctttcaggt tataagttgc cagcgcaggt ttatcttcgg cgaaacggcg gtcaatttcg
     5701 tccattcgat cccacgggtt atcgatagat tttccttcgc ctaatagctc ctgagcgaca
     5761 gtttccagcg agaatgaaga gaaattccag aacgcggatt tcagcgcctc gatactgtcg
     5821 ataattagcc gacctttagc ctgggcaaaa aagacgccgt ttttaaagcc gtgctcgcgc
     5881 cactccagct cactgttatc gcgccccagc cgcagcggaa tacgataacg ctcggcatgt
     5941 ttttgcagca ttcgcagatc aaactgcacc acgttccaac cgatgatcac atcaggatcg
     6001 tagtttgcaa accaggcgtt gagtttttcc agtagctgcg ggcggctggc gacgtactcc
     6061 agttcaaaat cgagcgcgga ggcgtcgcca ttctctggtc ccagcatata aacgatgcgc
     6121 tgcccgcagc cctccaggcc gatgcagtac agctctccgt ggcgggtggt ttcaatatca
     6181 atagaaaccc acttgagcgg cggacgatag tcgggatgcg gtttcagacg ggcattaacg
     6241 atagcgccat tgtgcatatc accctcgacc cacaccggtg aggtgataaa ccgctccatc
     6301 agatagcgtt ctggcgggcg cacatcggcc tcgtagacgg taacgccacc ttcacgcagg
     6361 cgcttttcgt aattcatcaa ctggcgatgg gcgcgacagt aaaggccata caccggctgg
     6421 cggtgaaaat cctttaacgc cagcggcgtc aggcgaaagc cttgttcacc ccgcaaaata
     6481 tgctgagcgc ggggaacctg atcggcggga ataaacgcca cggactcttg cggtgcaagc
     6541 gtaacctgca acggcccgtt gtccgtcgcc agccagaagg agacctctgt tccttgcggg
     6601 gtatcccgcc agtgtcgggt taagataaaa cctgcctgcg ccacgctgaa aatccatcaa
     6661 aaaaccaggc ttgattatag cctggtttca tttgattggc tgtggtttta tacagtcatt
     6721 actgcccgta atatgccttc gcgccatgct tacgcagata gtgtttatcc agcagcgttt
     6781 gctgcatatc cggtaactgc ggcgctaact gacggcagaa tatccccata taagcgacct
     6841 cttccagcac gatggcgtta tgcaccgcat cttcggcatt tttgccccat gcaaatgggc
     6901 cgtgagaatg gaccagaacg ccgggcattt gcgctgcatc gataccctgt ttttcgaagg
     6961 tttctacgat gacgttaccg gtttcccact catattcgcc gttgatttct gcgtcggtca
     7021 ttttgcgggt gcagggaatg gtgccgtaga aatagtcggc gtgggtggtg ccggttgctg
     7081 gaatcgactg acccgcctgc gcccagatgg tggcgtggcg cgagtgcgtg tgcacaatgc
     7141 cgccaatgga cgggaatgcc tgatagagta gtcggtgagt tggcgtatcg gaggagggct
     7201 ttttcgcacc ttcaaccact tcaccggttt cgatgctgac cacgaccata tcgtcagcgg
     7261 tcatgatgct gtaatcgacg ccggaaggtt tgatcacaaa gacgccgcgc tcgcgatcaa
     7321 cggcgctgac gttgccccat gtgagcgtga ccaggttgtg ttttggcagc gccaggttgg
     7381 cttctaatac ctgacgtttg agatcttcta acatgttgac tccttcgtgc cggatgcgcg
     7441 ttgcttatcc ggcctacaaa atcgcagcgt gtaggccaga taagacgcgt cagcgtcgca
     7501 tcaggcgctg aatgccggat gcgcgttgct tatccggcct acaaaattgc agcgtgtagg
     7561 cctgataaga cgcgccagcg tcgcatcagg cgttacatac cggatgcggc tacttagcgg
     7621 cgaaacccgt aatacacttc gttccagcgc agcgcgtctt taaacgctgg caggcgggtg
     7681 tcgttatcaa tcaccgtgat ttcaatgtcg tgcatctcgg cgaactggcg catatcgttg
     7741 aggttcagcg catggctgaa gacggtatgg tgcgcgccac cagcgaggat ccacgcttcg
     7801 gaagcagttg gcagatccgg ttgcgctttc cacagcgcat tcgccaccgg cagtttcggc
     7861 agggagtgcg gtgttttcac cgtgtcgatg cagttaacca gtagacggta acgatcgccg
     7921 agatcaatca agctggcgac aatcgctgga ccggtttggg tattgaagat caggcgggca
     7981 ggatcgtcct taccaccaat accgagatgc tgaacgtcga ggatcggttt ctcttctgcg
     8041 gcgatcgacg ggcagacttc cagcatatgg gagccgagca ccaggtcatt gcctttctcg
     8101 aagtgatagg tgtagtcctc cataaaggag gtgccgccct gcagaccggt tgacatcacc
     8161 ttcatgatgc gaagcagggc ggcagttttc cagtcgcctt cgcccgcaaa gccgtaaccc
     8221 tgctgcatca gacgctgtac ggccagacca ggaagctgtt tcagaccgtg caaatcttca
     8281 aaggtggtgg tgaacgcgtg gaagccacct tgttccagga aacgtttcat ccccagctca
     8341 atacgcgccg cttccagcac gttctgtcgt ttttcgccgt ggatttgtgt ggcaggcgtc
     8401 atggtgtagc agctttcgta ctcatcgacc agcgcgttaa catcgccgtc gctgatggag
     8461 ttcaccacct gcaccagatc gccaaccgcc caggtattga cggagaaacc gaacttgatc
     8521 tgtgcggcaa ctttatcgcc atcggtgacc gccacttcac gcatgttatc gccaaatcgg
     8581 cagactttca gatgacgggt atcctgttta gataccgcct gacgcatcca ggagccgata
     8641 cgctcatggg cttgtttatc ctgccagtga ccggtaacca cggcatgttg ctgacgcata
     8701 cgcgcgccaa tgaagccgaa ctcgcgaccg ccgtgcgcgg tctggttcag gttcataaag
     8761 tccatatcga tgctgtccca cggcagcgcc gcgttgaact gggtgtggaa ttgcagcaac
     8821 ggtttgttga gcatggtcag gccgttgatc cacattttgg ccggggagaa ggtgtgtagc
     8881 cacaccacca gaccagcgca acgatcgtcg taattagcat cacggcaaat agcggtgatt
     8941 tcatccggcg tggtgcccag cggtttcagt accagtttgc agggcagttt cgcttccgta
     9001 ttcagcgctt tcacaacgtg ctcggcatgt tgggtgacct gacgcagggt ttccgggcca
     9061 tacagatgct ggctgccaat tacaaaccac acttcataat tatcaaaaat cgtcattatc
     9121 gtgtccttat agagtcggaa cggcctgggc agcctgtgcc ggggcggaag ttggaagata
     9181 gtgttgttcg gcgctcatcg cccattgctg atagcggcga taaagctgtt caaagcgttg
     9241 tgcctgttcg ctgcgcggtt gcagggtttt ctctaccgca ctggccattt tttgctgggc
     9301 tgatgggatg tctgcgtgca ctttcgcggc gacggcagca aaaatcgccg caccgagcgc
     9361 acagcattgg tcagaggcaa caatttgcag cgggcgattc agcacgtcgc agcaggcctg
     9421 cataatgacc tggtttttcc gcgcgatgcc gcccagcgcc atcacgttat tgacggcgat
     9481 cccctgatcg gtaaagcact ccatgattgc gcgtgcgcca aaggcggtgg cagcaatcaa
     9541 accgccgaac agcagcggag cgtcggtagc gaggttaaga tcggtaatca cccctttcag
     9601 gcgttggtta gcgttcggtg tgcggcggcc gttaaaccag tcgagcacca ccggcaggtg
     9661 atccagagac ggatttttgg cccatgcttc ggtcagcgcc ggaagcagtt gtttctggct
     9721 ggcgtcgatt tgctctttta gttccggatg ctgggcggca agctgttcca gcggccagcc
     9781 gagtacgcga ccgaaccagg cgtagatatc accaaacgcc gattggcctg cttccagacc
     9841 gataaatcca ggcaccacgc tgccatcaac ctgaccgcaa atacctttca ctgcccgctc
     9901 gccaacgctc tgtttgtcgg caatcagaat gtcgcaggtg gaagtaccga taacttttac
     9961 cagtgcgtta ggctgtgcgc ctgcaccaac tgcgcccata tggcagtcaa acgcgccgcc
    10021 ggaaatcacc acgctttcag gcaggccgag acgctgcgcc cattccgggc ataaggtgcc
    10081 caccggaata tcggcagtcc aggtatcagt gaacagcggg gaaggcaaat ggcgattgag
    10141 gatcggatcc agctcatcaa agaaactggc tggcggcagg ccgccccagc tttcgtgcca
    10201 tagcgactta tgcccggcgc tgcaacgtcc gcgacgaata tcctgcgggc gcgtggtacc
    10261 ggaaagcaga gctggcaccc agtcgcacag ctcaatccac gatgcggcag attgcgccac
    10321 ggcgctgtcc tggcgagtca catgcaggat ttttgcccag aaccattcgc tggaataaat
    10381 accgccaata tagcgggagt agtcaacatt gcccggcgcg tggcacaaac gggtaatctc
    10441 ttccgcttct tcaaccgcag tgtggtcttt ccacaatacg aacatcgcgt tcgggttttc
    10501 ggcaaactcc gagcgcagcg ccagcacgtt accgtcggca tcaatcggtg cgggcgtcga
    10561 gccggtactg tcaacgccaa tcccgaccac agctgcgcgc tgttcgacgc taagttctgc
    10621 aagcacggtt ttcagtgccg cttccattga ctcaatgtag tcacgcggat gatgacggaa
    10681 ctggttattc ggggcatcac aaaattgccc tttttgccaa cggggatacc actctacgct
    10741 ggtggcgatt tcttcaccgg tggtgcagtc caccgccaaa gctcgcacag aatcactgcc
    10801 aaaatcgagg ccaattgcaa tcgccatcgt ttcactccat ccaaaaaaac gggtatggag
    10861 aaacagtaga gagttgcgat aaaaagcgtc aggtaggatc cgctaatctt atggataaaa
    10921 atgctatggc atagcaaagt gtgacgccgt gcaaattatc aatgtggact tttctgccgt
    10981 gattatagac acttttgtta cgcgtttttg tcatggcttt ggtcccgctt tgttacagaa
    11041 tgcttttaat aagcggggtt accggttggg ttagcgagaa gagccagtaa aagacgcagt
    11101 gacggcaata tctgatgcaa tatggacaat tggtttcttc tctgaatggc gggagtatga
    11161 aaagtatggc tgaagcgcaa aatgatcccc tgctgccggg atactcgttt aacgcccatc
    11221 tggtggcggg tttaacgccg attgaggcca atggttatct cgattttttt atcgaccgac
    11281 cgctgggaat gaaaggttat attctcaatc tcaccattcg cggtcagggg gtggtgaaaa
    11341 atcagggacg agaatttgtc tgccgaccgg gtgatatttt gctgttcccg ccaggagaga
    11401 ttcatcacta cggtcgtcat ccggaggctc gcgaatggta tcaccagtgg gtttactttc
    11461 gtccgcgcgc ctactggcat gaatggctta actggccgtc aatatttgcc aatacgggtt
    11521 tctttcgccc ggatgaagcg caccagccgc atttcagcga cctgtttggg caaatcatta
    11581 acgccgggca aggggaaggg cgctattcgg agctgctggc gataaatctg cttgagcaat
    11641 tgttactgcg gcgcatggaa gcgattaacg agtcgctcca tccaccgatg gataatcggg
    11701 tacgcgaggc ttgtcagtac atcagcgatc acctggcaga cagcaatttt gatatcgcca
    11761 gcgtcgcaca gcatgtttgc ctgtcgccgt cgcgtctgtc acatcttttc cgccagcagt
    11821 tagggattag cgtcttaagc tggcgcgagg accaacgcat cagccaggcg aagctgcttt
    11881 tgagcactac ccggatgcct atcgccaccg tcggtcgcaa tgttggtttt gacgatcaac
    11941 tctatttctc gcgagtattt aaaaaatgca ccggggccag cccgagcgag ttccgtgccg
    12001 gttgtgaaga aaaagtgaat gatgtagccg tcaagttgtc ataattggta acgaatcaga
    12061 caattgacgg cttgacggag tagcataggg tttgcagaat ccctgcttcg tccatttgac
    12121 aggcacatta tgcaagcatt gctggaacac tttattaccc aatccaccgt gtattcattg
    12181 atggcggtgg tgttggtggc ctttctggag tcgctggcgc tggtcggttt gattctaccc
    12241 ggtacggtgc tgatggcggg gctgggagcg ctgattggca gcggcgagtt aagtttctgg
    12301 cacgcctggc tggcagggat cgtcggttgt ctgctgggcg actggatctc cttctggctg
    12361 ggctggcgtt ttaaaaagcc attgcatcgt tggtcatttc tgaagaagaa caaagcatta
    12421 ctcgataaaa ctgaacatgc gttgcatcaa cacagcatgt tcaccattct ggtcggtcgt
    12481 tttgttggcc cgacgcgtcc gctggtgcca atggtggcgg gaatgctgga tctcccggtg
    12541 gcaaaattta tcacgccgaa tattatcggc tgcctgctgt ggccaccgtt ttacttcctg
    12601 ccggggattc tggcgggtgc ggcgatcgat attccagccg gaatgcagag cggtgagttt
    12661 aaatggttgc tgctggcgac ggcggtattt ttgtgggttg gtggctggct gtgctggcgg
    12721 ttatggcgca gcggcaaagc aaccgatcgt ttgagtcatt atttgtcccg cggtcgtttg
    12781 ttgtggctga cgccgttgat ttctgccatc ggcgtggtgg cgctggtggt gttagttcgc
    12841 catccgttaa tgccggtgta tatcgatatt ttgcgtaaag tggttggagt ttagggcata
    12901 gtcttgtgtg gggcgcttga tgcggacaaa acgcacccgt aggtcggata aggcgttcac
    12961 gccgcatccg acagtgcata ttaacctgta attcccaaca gcgccgaagc actcgcctta
    13021 ccgctcaaca actcattggt cataccctgc caggcgattc gcccgtcggc gactactacc
    13081 gagcgcgtgg caatccgcgc cgcatcttcc acactgtgcg acaccatcaa tagcgtcatt
    13141 ttttgctgct ggcagctagt gctcaccagc gtcaacatct cctgacgtag cgccggatcg
    13201 agcgcagaga acggttcatc gagcaataaa atcggctgtt cgcgtaccag acaacgcgct
    13261 aacgccacgc gctgtcgctg accgccggaa agctcgcccg gtaaccgcgc cattaaatta
    13321 tcaatcccca tctgacgggc aatcgcgtgc attttctcct gctgtaccgc gttcagcttc
    13381 aatcctggat taagccccag cccgatgttc tgcgcgaccg tcaggtggct gaacaggttg
    13441 ttctcctgaa acagcatcga caccggacgg cgtgacggcg gcgtcgtggt gtgatcaacg
    13501 ccgtcgatag tcagcgcacc gctggctggt gtcagaaaac cggcgatcaa gttcagcagg
    13561 gtacttttac ccgcgccgct tggcccgagg atcgccacct gctcgccgcg ttctaccgtt
    13621 aagctaaaac gcatcggcaa atggtgataa agccaggtga tatcagtcag ttttaacatt
    13681 tcgccccggt attttttcaa tcacgctaaa cagcagaaaa cagagcagca gcagaattaa
    13741 cgcggtgacc gcgccgtcct ggctgcgata ggagccaatt tgctggtaga gataaaacgg
    13801 cagggtgcgg aaatcatcgt taccgaacaa cgccaccacg ccaaaatcac caatcgacag
    13861 cacgcaggca aaggccagcg cctgcgccag cgggcgtttc agggcgcgca gctccactac
    13921 ttttaagcgt gaccagcctt caatccccag cgactgacat aacatgctgt agcgggcggt
    13981 gatatcgcgc atcgggtttt ccagcacttt cagggcgtag gggatcgcca ttaacgcatt
    14041 ggtgaaaatc acaatgccgt cagcagattg tggcaggccg atagtgttgt tgagcagtaa
    14101 aaagaagccc gttgccagca caatccccgg catagcgagg atcaacatgc cgctcatctc
    14161 cagcgcctgg cccgccagca ttttttgccg cgcccgcagt tcgcgactgc tccatagcag
    14221 catcatggtc agcactacgc acaatacacc tgccgccagc gcaatacgca acgaggtcca
    14281 cagcgcctgc cacagcaccg gttgtgccag cacttccggc aactggcgat ttaccccatc
    14341 gacgatcacc gccagcaacg gtggcagcaa cagcagcagc gccagcacaa ttaacatcgt
    14401 gtcgcaaatg cggctatgca gacgatcgtc cgggtcgcgc cagccttgca gcagcgtggt
    14461 gccgggcgca atggccttac tcagtcgctg actcaacagc accagcccaa ggcaacacac
    14521 catctggatc agcgccagca tcgccgcgcg ggcaggatcg tagtcgtaac tcagcgcctg
    14581 atagattgcc agctcgatag tggttgcctg cggaccgccg cccagcgata acacggtggc
    14641 gaagctggcg aaacagagca taaagataag cgcggtaacc ggcgggattt gtcgccgtaa
    14701 ccacggccat tcgacgaagc ggaaaaaatg ccagccgcgc atcccaagct gggcggcaag
    14761 ctgacgttgt tcgccgggaa tgttttccag tgcctggagt aataagcggc tcgccatcgg
    14821 cagattaaaa aacacatggg ccagcaaaat gccttgcagg ccgtagggcg aaaaggtcca
    14881 ctccagaccg agcgattggc agagcgatgc cagccagccc tggcgaccat agacgctaag
    14941 aatgccgaaa acagcaacca gcaccgggag gatcaaggtc attgcgcaca gacgcaacag
    15001 cgccagccga cccggaaagc gcctgcgata gagcgcgcgg gcgaggaata tcgcgggtac
    15061 gacagagagt agcgccgaga gaaacgcctg ccagaaggag aagcgcacca catgccacag
    15121 gtagctgtcc tgccagactg ccgaccagtc accctgcggc gcgttccacc acagggcgag
    15181 aaacgccgcc agcgcaaccg ctaccaccag cgtggcggcg cttacacctg gaattaacca
    15241 gccgggaatt aacggctgac ggcgcgttgc cattcgctaa tccatgcctg acgttgtgcc
    15301 gccacttcgg ctggcgtgaa ctccagcgtg gttgcgggtt tggtcaattt ttcaaaaccg
    15361 gcaggcagcg tgacgtttgc caccggatac atccagttgc cggttgggat cgcattctgg
    15421 aaagccggag aaaccataaa ctggaggaat ttttgcgcca gctccggctg cttgctggca
    15481 gcggtgcgag cggcgacttc cacttgcaga tagtgacctt cgctgaagtt cgcggcggcg
    15541 tagttatctt tcttctcttc gagaatgtga taagccggag aggtggtgta actcagtacc
    15601 agatcgcttt caccttttaa aaacaggccg taggcttcgc tccagccttt ggtgaccgtg
    15661 accgttttct tcgccagttt ctgccaggct tgtggggcgt catcgccata gactttttgc
    15721 atccatagca acagacccag ccccggtgta ctggtgcgcg gatcctgata aatcacccgc
    15781 cagttttgat cgctctcaac cagttctttc aggctttgtg gcgggttttt cagtttgttc
    15841 ttgtcataaa cgaaggcgaa gtagccataa tcaaacggta cgaaaatgtc attattccag
    15901 ccgccgggaa cgttaacggc atccgctgcc acaccgcttt tggcaaacag tccggtttta
    15961 ctggcggcgt ctaacagatt gttatccagc cccagcacca catcggcttt actgtttttg
    16021 ccttccatcc gtagacggtt gagaagcgaa acgccatctt ccagcgccac cagtttcagt
    16081 tcgcaattac agtcggcttc aaaggctttt ttaaccaccg gaccaggccc ccagtcggcg
    16141 gcgaaggaat cgtaggtata aacagtcaga acgggtttag cgaaaacggg cgctgtgcac
    16201 agcaacagca ggggaagaca tttttttaac actttgcacc tcaaaaaaga gtggcaaagg
    16261 acttgagaag gagcctcaaa tcccttcgcc ggcgttatcc ggatcaggtt cgacgggtat
    16321 tttctcagcg cacgcgtccg cgtggcaccc cgttgagaac ggcgttagtg tagtgatttt
    16381 gttatcaacc agcaatccat agagaaggcg tattaaaaaa gccaatattc gttcagctat
    16441 caagagcgta tttgcatagc tggagagcgt tgatccgaaa gaatcccttc gctacagtca
    16501 aaataggtaa cgtaaccgtt tattgcgtga atgagcgatt tttgacagta ttcttattca
    16561 ggacgcttat tctgagtttt ttattcagag tggagctatc agcatgaaag taagaaaccc
    16621 agaacagatt agtatccctg ctagtaatac cactaaagat ccaggcctta ctaattctca
    16681 gattatcagg atggctaact tggtcaaaaa gaccgagaat atgaatatct ttgaagagct
    16741 atgggagacg ttacgcaatc tcttccagtc agataaacat tcccagactg cggcaaggca
    16801 aattctgaag gatgcatttt atttccagaa tagtgatgac tactcaaaat actttaccgg
    16861 ggctgtcgac gggaaggcgc gtgataagtt aactcactgg ctgataaaat ttaatgaact
    16921 caaagaatac gcaaaagatc cggaaaatat ggccgcgaag gcctcactct cgccagaagg
    16981 tacgttatgc gtcagttttt ttattggcga tgaggcgata tttactctgg agttgcagct
    17041 gaaaaagagc accaggaccg gggggattga tctgagtaat gcttacttta acggggttgt
    17101 catttgtggt attgactgtc tcgaggttga cctgagcaat gctgaaacga acaatagccg
    17161 ttggtatgac tgacctgcgc gaggttaacc tgaaaaagct gacctttccg gccagaacaa
    17221 ggctagactg cgccgttatt gattaactca gaatatgaaa gaggggctgg aatgcccctt
    17281 ctttaatctt atttgcgacg ctaatcagac gatgcataaa acagagaaaa tataaaagta
    17341 atgtatttta tcattccgtc tgaatataaa aaatgcgctc atgaccgcca aaaggcgcaa
    17401 gctactctgg atccggcggc gcaaaccacg ctgatttaaa atcaaaccag ccgagggtgt
    17461 tcatgcgcag gccgcgcata ctgcgttgtc cctgaatgat cagccagtgg tggataagtg
    17521 gcaccattgc tttgctggcg accagttgtt ggcaccagtt cgccaggttc atttcgccat
    17581 tgcgccagcg ggcggcgtcg acttgccagt cgattggaat acagtgttgg agcagcggta
    17641 cctcgcacag gtgcgcgaac agcgaaaaat ccagcggcag cgtaaagttg gcgctgttaa
    17701 gccagatatc gctctcgatc tctccttcat gccactgatc gtagctgatc tctttgattt
    17761 ccagtgtgac ctggtggctt gccagaatct gctgcatgat cccggcaatc acccgatgct
    17821 cactgtgatc ctgataaaag gtcagggtga ggctttccag accagccggt ttttcgctcg
    17881 ttatggtgcg ggcatgatgc caacggggga gcagtccata agccgggaac cacagttgct
    17941 ggtactgttc ctcagcgaaa tagaccagat tagttggaga aagcacatag cttacccagt
    18001 ccctgacttg ctgattcgcc ccgcgatggg tgcggctatc gaacagtaaa tagtagcaac
    18061 cttcctccag gcggctttca atctcttttt cctcgccctg tggacctttt agcatcagcc
    18121 ctccggctgg ctcgtcggca atttccggca gaacccagac gttaacttca tcgattaatg
    18181 cccggtaacc gaagaagtca tcgaatgcct gaattttcag ttgattggtg ctgttgcgaa
    18241 tcaccgcata cggaccggtg ccgatgggat ggctggcaaa gttactgagg gtttcccatt
    18301 cgcgcggcag gatcatcgcc ggaacttgtc ccagcagtaa cggcaaccag cgatccggct
    18361 gcgtgaggtg gatatccagc gtccagggcg tcggcgacac aatgtcagca atatgcgaat
    18421 agagcggcag cgtattgatt cgttttaaag aggcgatcac gtcgtccatt tccagttcac
    18481 gaccatgatg aaaatggact cctggacgca aaaagaaacg ccagtgaagc ggtgatattt
    18541 gctgccagtg gtgggcgatg tctgcttcca gttccccatt ttcctcattt atgcgcgtta
    18601 gcgaactgaa gatttgccgg gcgatatggg tttcggaacg gcgcaatgcg ctgccaggta
    18661 gcagattacg caacggacga tagtagagca cgcgcaggat gtgccgcccc tggcggaagc
    18721 tgcggcccag atgagaaacc agcatttgcc tcacagtcgc tttgtcgcca actaactgca
    18781 ctagttggtc gatacggtcc tgctccagca ggtcttccgc ccgctgttgc tgaagcgcca
    18841 gcccggtata gaggaatgtc agacgcgagc gtttaccacg cccgacttcc gcttcccacg
    18901 tcagccagcc gcgatcctgc atggtgttga gcagggtacg catatgacga cgcgagcagc
    18961 tcaataacgc tgccagttcg ttgagcgttg tgtcctgcga tttaccctcg cagcattgcc
    19021 acaggcggat gaactgttgt tgcagacgag cagatggcat aaaaggggaa ctcctgcgca
    19081 aaagacagca attttatttt ccctatatta agtcaataat tcctaacgat gaagcaagga
    19141 ggtgccccat tcgtcagttt tatcagcact attttaccgc gacagcgaag ttgtgctggt
    19201 tgcgttggtt aagcgtccca caacgattaa ccatgcttga agaactgatg cagtgggagg
    19261 ggagtcattc tgactactga tttgcggaca tcatgtgtga ctgagtattg gtgttaatcg
    19321 ccacgccagc agtgattatc tgctggcttt tttctttttg gcgattgagt atcggacggt
    19381 gtacaaacta acgcataaac gcgggttgct tcgcttcata agccgcaatg gcgtcgtcgt
    19441 gctgcaaggt aagcccaatg ctgtccagac cgttcatcat gcagtggcgg cggaaggcat
    19501 caatggtaaa gcgataggtt ttctcacccg ctttcacctc ttgcgcttcc aggtctacat
    19561 caaaatgaat ccccggattg gctttcacca gcgcaaacag ctcatccact tctgcgtcgc
    19621 ttaatttcac cggcagcagc tggttgttaa agctattgcc gtagaagatg tcagcaaaac
    19681 tcggcgcaat caccacctta aaaccgtagt cggtcaatgc ccagggcgcg tgctcacgcg
    19741 aggagccaca gccgaagttt tctcgtgcca gcaaaatgga agcgccctga tactgcgaga
    19801 agttcagcac gaagtccggg tttggctgtt ggcctttttc atccagaaaa cgccagtcgt
    19861 taaacagatg cgcgccaaaa ccagtacggg tcactttctg caaaaactgt ttcgggatga
    19921 ttgcatcggt atcgacattg gcggcatcca gcggaaccac caggcctgtg tgtttgataa
    19981 atttctctgc catggtgtgc tccttattta atgttgcgaa tgtcggcgaa atggccggtc
    20041 acagccgcgg cggcagccat tgccgggctg accagatgcg tgcgcccgcc gcgcccctga
    20101 cggccttcaa agttacggtt gctggtggag gcacaacgtt cgcccggatt cagacggtcg
    20161 ttgttcatcg ccagacacat tgagcagcca ggcaagcgcc attcaaaacc ggcttcaata
    20221 aagattttat ccagaccttc cgcttccgcc tgcgctttta ccggaccaga gccgggaacc
    20281 accagcgcct gtacgcctgg cgcgactttc cgccctttgg cgatttccgc cgccgcgcgt
    20341 aaatcttcaa tacgtgagtt ggtgcaggaa ccgataaaca ctttgtcgat agccacttcg
    20401 gtcagcagaa tacccggttt cagccccata taggccaacg ctttttctgc cgacgcgcgt
    20461 tcaaccggat cggcaaacga agccggatcg ggaatattgt cgttcacgga aatcacctgg
    20521 cctgggttag ttccccaggt gacctgcggt gaaatctctt ctgcctgcaa agtgacaacg
    20581 gtatcgaaag ttgcgccttc gtcggtttgc agggttttcc agtaggcaac ggcgtcgtcg
    20641 aaatctttgc ctttcggcgc atgcagacgg cctttgacat agttaaaggt ggtttcgtca
    20701 ggtgcaacca gaccggcttt cgcgcccatt tcgattgcca tattgcacag ggtcatccga
    20761 ccttccatgc ttaaatcacg gattgcttcg ccgcaaaact ccaccacatg cccggtgccg
    20821 cctgcgctac cggttttacc gataattgcc aacacgatat cttttgcggt aatgcccggc
    20881 gcggctttgc cctggacttc aattttcatg gtcttcgcac ggccctgttt cagggtttgc
    20941 gttgccagta cgtgttcaac ttcggaagtg ccgataccaa aggccagtgc gccaaacgcg
    21001 ccgtgggtgg cggtatgcga gtcgccgcag acaatggtca tccccggcaa ggtcacgccc
    21061 tgttccggcc ccattacgtg gacgatccct tgataggggt gattcaggtc atacagttcg
    21121 acgccaaatt ctttgcagtt tttgatcagc tcctgcatct ggatgcgcgc catttcaccg
    21181 caggcattaa tgtctttagt ctgggtagag acgttgtgat ccatggtggc gaaggttttg
    21241 cccggctgac gtaccgggcg accgtgggcg cgcagaccat cgaacgcctg cggtgaggtc
    21301 acttcatgca ccaggtggcg gtcgatatat aacagcgggg tttcgttttc ggcttcgtac
    21361 actacgtgag cgtcgaataa tttttcgtat aacgtcttag ccatgattac accccttctg
    21421 ccacatagcg ggcaatgata tcgcccattt catcggtact aacggcggca gcgccacggg
    21481 ctaaatcccc ggtgcgaatg ccttcttcta atgcgcggtt aatggcgcgt tcaatggcgg
    21541 aagccgcatc atcggcatcc aggctgtaac gcagcagcag tgccagcgac agaatttgcg
    21601 caatcgggtt ggcgatgttt ttacctgcga tatctggtgc cgagccgccc gccggttcat
    21661 acagtccaaa accttgctcg ttcaggctgg cggaaggcaa catccccatc gagccagtga
    21721 tcattgcgca ctcgtcagac agaatgtcgc caaacaggtt ggagcacagc agaacgtcaa
    21781 actgtgatgg atctttaatc agctgcatgg tggcgttgtc gatgtacata tgcgccagtt
    21841 cgatatccgg gtattccgtg gcgatctcgt tgacgatttc acgccataaa atagaggatt
    21901 gcagcacgtt ggctttatcg atcgacgtca ctttatggcg acgtttacga gcagattcaa
    21961 acgcgatgcg ggcaatacgc tcgatctcaa aacggtgata cacctcggta tcaaacgctt
    22021 tttcatattg tccgctacct tcgcggcctt ttggctgacc gaaatagatg ccgccggtca
    22081 gttcgcgcac gcacaggatg tcgaagccgt ttgcggcaat gtctgcacgc agcggacaga
    22141 atgcttccag cccctgatac agttttgccg gacgcaggtt gctgaataat ttgaagtgct
    22201 tacgcaaagg caacagcgcg ccgcgttctg gttgctggtc cggtggtaaa tgttcccatt
    22261 tcgggccgcc taccgagcca aacagcacgg catcggcttg ctcacaacct tcaaccgtcg
    22321 caggcggcag tggttgcccg tggttatcaa tggctgcgcc gcctacatcg taatggctgg
    22381 tagtgatgcg catcgcaaag cggttgcgca cggcatccag cactttcagc gcctgggtca
    22441 tcacttccgg accaatgcca tcccccggca atacggcaat atggtaattc ttcgacatca
    22501 cacggtttcc ttgttgtttt cgttgtgttg agctttgcgt tgcaactctt tttcgacttc
    22561 tgtggcacgc cagatattgt taagtacgtg caccatggct ttggcggagg actcgacaat
    22621 atcggtggcc aggccgacgc cgtggaagcg gcgaccgttg tagttggcga caatatccac
    22681 ctgacccagc gcatctttac cgtgaccttt ggcggtcagg ctgtatttca ccagttcgac
    22741 gttatagtca gtgatgcggt taatcgcctg atagacggca tcgaccggac cgttaccgtt
    22801 ggcggcttct gctttgactt cttcgccgca ggccagtttg acggcggcgg tggcgatatc
    22861 gttagagcca gactgcacgc tgaagtaatc cagacggaaa tgctccggct cttcttgctg
    22921 cttaccgatg aaggccagcg cctccagatc gtaatcaaac acctgacctt ttttgtccgc
    22981 cagcttgagg aaggcgtcgt acaaattgtc taaattatat tcactttctt tataccccat
    23041 ctcatccatg cgatgtttca ccgccgcacg ccccgaacga gaggtcagat tcagctggat
    23101 ttggttcaga ccaatagatt ctggtgtcat gatttcgtag ttttcgcggt ttttcagcac
    23161 gccatcctgg tggataccgg aggagtgtgc gaatgcgccg ctgccaacaa tggctttgtt
    23221 tgccgggatc ggcatattac aaatctggct aactaactgg ctggtgcgcc atatctcctg
    23281 gtgattaatg gcggtgtgga cgttgagaat atccttacga actttaatcg ccatgatgac
    23341 ttcttccagg gaacagttac cggcacgctc gccgatcccg ttcattgcac cttccacctg
    23401 gcgtgcaccg gcatgtaccg ccgccagtga gttgccgacc gccaggccca aatcgtcgtg
    23461 ggtatgtacg gaaataatgg ctttgtcgat gttaggcacg cgttcataca ggccgctaat
    23521 gattccggcg aactcaaacg gcatggtgta gcccacggtg tccggaatgt tgatggtggt
    23581 ggcaccggca ttaatcgccg cttcgaccac tcgcgccaga tcggcaatgg gtgtacgccc
    23641 ggcatcttcg caagaaaatt caacatcatc ggtgtaatta cgggcgcgtt tcaccatata
    23701 gatagcgcgt tcgatcacct cgtccagcgt gctgcgcagc ttggtggcga tgtgcattgg
    23761 cgaagtggca ataaaggtat gaatacggaa ggcttcggcg actttcaggg attcggccgc
    23821 cacgtcgata tctttctcca cgcagcgagc taacgcgcat acacggctgt ttttaacctg
    23881 gcgagcgatg gtttgcactg attcaaaatc acccggcgaa gagacgggga acccgacttc
    23941 catcacgtca acacccatac gctcaagggc cagcgcaatt tgcagttttt ctttcacact
    24001 caagcttgcc tgtaacgcct gttcaccgtc gcgcaatgtg gtatcgaaaa taatgacttg
    24061 ctggctcatg gtttgggtcc ttgtctcttt tagagcgcct cgcttcgggc ataaaaaaac
    24121 ccgcgcaatg gcgcgggttt tttgtttgac tgcgtgctgg cttaatgctg gatgccgctc
    24181 actcgtctac cgcgcaaaga agatgcgttt agtagtagta gtagaccgat aaagcgaacg
    24241 atgtgagtca ttaaatcagc tccagatgaa tgcgatatgc ttttagagtt actggataca
    24301 aaaacggatg tcaaccctga cgcaataaaa acgtcccgcc agcatgagtt ctgcatccgt
    24361 aagattagct aattgtgctg ctgtggttaa aacaagcgat attaatttct gcttaactac
    24421 cgatgctttt catcggttga catatttcag aataaatttt tgcatctaat ccacgaggaa
    24481 aaaggggaca aaatgcacgc gttgcaaaac ctatcctgat gatttgtatt gaattatatg
    24541 ttttgcgga
//
LOCUS       DAZFAB010000069        24342 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZFAB010000069 DAZFAB010000000
VERSION     DAZFAB010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24342
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.069"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            330..3026
                     /gene="stcE"
                     /locus_tag="ACHJWA_003083"
     CDS             330..3026
                     /gene="stcE"
                     /locus_tag="ACHJWA_003083"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M66 family glycomucinase StcE"
                     /protein_id="HFO4309632.1"
                     /translation="MNTKMNERWRTPMKLKYLSCTILAPLAIGVFSATAADNNSAIYF
                     NTSQPINDLQGSLAAEVKFAQSQILPAHPKEGDSQPHLTSLRKSLLLVRPVKADDKTP
                     VQVEARDDNNKILGTLTLYPPSSLPDTIYHLDGVPEGGIDFTPHNGTKKIINTVAEVN
                     KLSDASGSSIHSHLTNNALVEIHTANGRWVRDIYLPQGPDLEGKMVRFVSSAGYSSTV
                     FYGDRKVTLSVGNTLLFKYVNGQWFRSGELENNRITYAQHIWSAELPAHWIVPGLNLV
                     IKQGNLSGRLNDIKIGAPGELLLHTIDIGMLTTPRDRFDFAKDKEAHREYFQTIPVSR
                     MIVNNYAPLHLKEVMLPTGELLTDMDPGNGGWHSGTMRQRIGKELVSHGIDNANYGLN
                     STAGLGENSHPYVVAQLAAHNSRGNYANGIQVHGGSGGGGIVTLDSTLGNEFSHEVGH
                     NYGLGHYVDGFKGSVHRSAENNNSTWGWDGDKKRFIPNFYPSQTNEKSCLNNQCQEPF
                     DGHKFGFDAMAGGSPFSAANRFTMYTPNSSAIIQRFFENKAVFDSRSSTGFSKWNADT
                     QEMEPYEHTIDRAEQITASVNELSESKMAELMAEYAVVKVHMWNGNWTRNIYIPTASA
                     DNRGSILTINHEAGYNSYLFINGDEKVVSQGYKKSFVSDGQFWKERDVVDTREARKPE
                     QFGVPVTTLVGYYDPEGTLSSYIYPAMYGAYGFTYSDDSQNLSDNDCQLQVDTKEGQL
                     RFRLANHRANNTVMNKFHINVPTESQPTQATLVCNNKILDTKSLTPAPEGLTYTVNGQ
                     ALPAKENEGCIVSVNSGKRYCLPVGQRSGYSLPDWIVGQEVYVDSGAKAKVLLSDWDN
                     LSYNRIGEFVGNVNPADMKKVKAWNGQYLDFSKPRSMRVVYK"
     gene            3113..3988
                     /gene="gspC"
                     /locus_tag="ACHJWA_003084"
     CDS             3113..3988
                     /gene="gspC"
                     /locus_tag="ACHJWA_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HFO4309633.1"
                     /translation="MLFFLSFRGDRGLFIKDIVLKMLTPNRLLCVILLIAGYQLVSVI
                     HHFWLTQAASVPGLSRVSAPETAVTGDQTEERFVFTLFGRASPLSSEGRAQETMPSLS
                     DDLLSGEDLDVRGILYSSVAEHSVAIFAHNNRQFSLSVGEKVPSYDATISAIFSDHIV
                     INYQGKTVSLPLRYDNTEKKNAYDNNNLTVGDVITQDNFRVESVFDIMSFSAVTVNNT
                     LSGYRLIPGKHSSLFYNAGLHDNDLAVSVNGSELRDTRQAQQIMKQLPELKEIKITVE
                     RDGQLYDAFIAVGEN"
     gene            3989..5956
                     /gene="etpD"
                     /locus_tag="ACHJWA_003085"
     CDS             3989..5956
                     /gene="etpD"
                     /locus_tag="ACHJWA_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="variant type II secretion system secretin EtpD"
                     /protein_id="HFO4309634.1"
                     /translation="MFRKWLNGRLPVLVFTTVILGAIPGWGAEFSANFKDTDIQEFIN
                     TVSKNLHKTVIINPDVQGTITVRSYDMLNEEQYYQFFLSVLDVYGFAVVDMHNGILKV
                     VRSKDAKTSAVPVASDVSPGTGDEVVTRVVPVSNVAARDLAPLLRQLNDNAGAGSVVH
                     YEPSNVLLMTGRAAVMKRLMEIVERVDKVGNRSVATVPLTYASATDVARLVTELTKET
                     DKTAIPAWMTAKLVADERTNSVLVSGEPISQQRIISIIKQLDRQEDVQGNTKVIYLKY
                     AKAKDLVEVLTGISSSIENDSKKSPSTEALRKGVTIKSHEQTNALILTGAPDVIRDLE
                     NVISQLDIRRPQVLVEAIIAEIQDADGLNLGIQWVNKHAGVAQFTSTGLPITTMVQTR
                     QNEILDSDQSNALSMFNGIAAGFYQGNWAMLLTALSTSSKNDILATPSIVTLDNMEAT
                     FNVGQEVPVLSGSQTTSGDNIFNTVERKTVGIKLRVKPQINEGDSVLLEIEQEVSGVA
                     DTAVATTTDLGATFNTRTVTNAMLVGNGETVVVGGLLDKSIRGSESKVPLLGDIPVLG
                     HLFRAKSEQTAKRNLMLFIRPTIIRERDGFRHASAEKYQSFNQEQVQSRGKETTALTL
                     NEEQLRLSPDQDDTAFRKVKAAIAAFYAQEM"
     gene            5956..7461
                     /gene="gspE"
                     /locus_tag="ACHJWA_003086"
     CDS             5956..7461
                     /gene="gspE"
                     /locus_tag="ACHJWA_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HFO4309635.1"
                     /translation="MSRVVQNVSESRPLLPFSFSRTQRILLLREQEGNRVFCMEDTPA
                     SALLEVRRVAEGPLNVTTVSAEAFEKQLVSSYQRDSDEARQMMAEIGNEMDFYTVAGE
                     LPDREDLLDANDDAPIIRLINAMLTEAIKEKASDIHIETYERHLQVRFRIDGVLREIL
                     RLHRNLASLLISRIKVMARLDIAEKRVPQDGRMVLRIGGRAVDVRVSTLPSNHGERIV
                     LRLLDKNSVSLDLAALGMSQQNQRHIDALIRRPHGIILVTGPTGSGKSTTLYAALSLL
                     NPRDRNIMTVEDPVEYELDGISQTQVNPKVDMTFARSLRAILRQDPDVVLVGEIRDGE
                     TAQIAVQASLTGHLVLSTLHTNSAAGALSRLQDMGIPPFLLSTSLLAVLAQRLVRTLC
                     PRCRQPCQVSTELAMDMDIPPETTIWQPAGCQHCSFTGYHGRTGIHELLLIDDRIRTA
                     IYQGEGELGITRLAGSRYLTLRGDGRQKVLAGETSWEEVVRVTESRLQEEE"
     gene            7463..8686
                     /gene="gspF"
                     /locus_tag="ACHJWA_003087"
     CDS             7463..8686
                     /gene="gspF"
                     /locus_tag="ACHJWA_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HFO4309636.1"
                     /translation="MALFHYQASDIHGRKRSGILEADSARHARQLLREQALIPVRLDE
                     KQVHHKHSLRSILRFRPRGGSSAELALLTRQLATLVAASLPLEEALDALLRQSEKPRQ
                     RNLIAAVRTKVLEGHSLAAAMGMFPGTFERLYCAMVAAGETSGRLDVVLSRLADYTEQ
                     RQIMRNRLLQALLYPCVLTLVAVGVIAILLTAVVPKVVEQFIHMKQTLPLSTRVLMGA
                     AEVSQTWGPWLLLAAALGGIAGRMILHQPSQRLAFHHLLLRLPVVGRISRGLNTARYA
                     RTLSILNASAVPLLQAMHISGDVLSNDWARHQLATAAELVREGVSLHQALEQTSLFPP
                     MMRHMIASGENSGELDSMLERAADNQDREFSTQMQLALGLFEPLLVVGMAGVVLFIVL
                     AILQPLLQLNNMMNM"
     gene            8717..9151
                     /gene="gspG"
                     /locus_tag="ACHJWA_003088"
     CDS             8717..9151
                     /gene="gspG"
                     /locus_tag="ACHJWA_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HFO4309637.1"
                     /translation="MRKQHQRGFTLLEIMVVIVILGVLASLVVPNLMGNKDKADRQKV
                     MSDLVALESTLDMYRLDNNRYPTTEQGLRALVSKPTVQPEPRNYRQDGYIRRLPQDPW
                     GGDYQLLNPGQYSDIDIFSPGPDGVPNTEDDIGNWTLGNAQP"
     gene            9148..9702
                     /gene="gspH"
                     /locus_tag="ACHJWA_003089"
     CDS             9148..9702
                     /gene="gspH"
                     /locus_tag="ACHJWA_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HFO4309638.1"
                     /translation="MSQRGFTILEMMLILLLMGITAGLVLMSFPDSAQNHLQQQRERL
                     QAQLDYALDRSQQDGLLMGIQVRFDGWKFKVLQRGTAESPPTLAEGGDIWQGYVWQTW
                     QPRRAAMGGKLPDDVRLELQYLRGLQWMSEHDDGAEPDILLLPGGEVTPFRLLFRQVG
                     EEAVVGLQVDENGLMTLFEGEVSL"
     gene            9699..10064
                     /gene="gspI"
                     /locus_tag="ACHJWA_003090"
     CDS             9699..10064
                     /gene="gspI"
                     /locus_tag="ACHJWA_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HFO4309639.1"
                     /translation="MKGQEGMTLLEVIVALVVFALAGMALMQASTQQAAGIGRMEEKV
                     LAGWLADNQMVQLQLEKTWPENGWGEKTISFAGTEWYLRWGGPDSDVPQPRSLEVEVR
                     RTKEETSALVSLRSSVVRE"
     gene            10061..10660
                     /gene="gspJ"
                     /locus_tag="ACHJWA_003091"
     CDS             10061..10660
                     /gene="gspJ"
                     /locus_tag="ACHJWA_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HFO4309640.1"
                     /translation="MSQQRVKGFTLLEMLLALAVFAALSISAFQVLQGGIRAHELSRD
                     KVQRLAELQRGISQMERDLTQMLPRHSRGNEGLLLAAPHLLKSDDWGISFTRNSWLNP
                     AGMLPRPELQWVGYRLRQQKLERLSYFHVDHPSGVSPDVRVMLDGVHAFRLRFFVNGD
                     WQARWDSTGILPQAVEVTLVMDDFAELPRLFLVSKETAE"
     gene            10657..11634
                     /gene="gspK"
                     /locus_tag="ACHJWA_003092"
     CDS             10657..11634
                     /gene="gspK"
                     /locus_tag="ACHJWA_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HFO4309641.1"
                     /translation="MKLREQGVALLVVLLILSLMVTIAAVIAERNGRTFLRTVAQLDQ
                     LQAKWDGYTAETIAKQILQRSRQESPRKTHLAQNWAQSERQFETRGGDVRGQIVDAQA
                     CFNLNAINYGVVDLTSIPYAARIFQQLLINLQVELLQARQVTAALRDWIDRDDKPVRG
                     GAEDEVYMGMEPPYLAANQPMQDVSELRLIRGIDARLYRKLLPYVCVLPTSDLSVNVN
                     TLLDSQAPLLAALFLTKPDSLPVTELLQRRPRTGWESVAAFLDAPPLKDIDTSAAMPV
                     LAVSSNYFLVRLHVRSGEHLFSQQTLMQWREERFRIIQRQYGLTMREVP"
     gene            11568..12845
                     /gene="gspL"
                     /locus_tag="ACHJWA_003093"
     CDS             11568..12845
                     /gene="gspL"
                     /locus_tag="ACHJWA_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HFO4309642.1"
                     /translation="MEGGAFPDHSATVWINDEGGSLKKNNKHVEAGRLFLRLPEDDSE
                     PLLWRLIAPEETGAGSVAVGETDPQLVGLLSRYPAWVLVPSGRIAFHQVTLPHRTWRQ
                     RLQVLPFLLEEQLATDIEQLHFAILHQSGDRCDVAVVEKAVMHRWLAYCGQLGVREVR
                     LLPDVLMLPLATEGWSAVKLAGQWLFRRDRYAGMTVELSWLTHFLSLTAPPVIESYSV
                     PPVPCPGPKTTEWRNQPGRDLLQLVAEGDGYDGADLRQGEFARQGTWYARFRPWRYVA
                     GALLACVFLAGSNAGLAHYRLWQQAQFWRYESVRVYQQLFPSEKAVKDPRRQMLRHLQ
                     QSTGNNIPELGSVMRQLQQLLSETSTIRLQALAWDSSSQTLKIDLQAASFQALEHFQQ
                     IAGEKYLIQPGEIRQQPQGVESRLILRVNNERA"
     gene            12832..13344
                     /gene="gspM"
                     /locus_tag="ACHJWA_003094"
     CDS             12832..13344
                     /gene="gspM"
                     /locus_tag="ACHJWA_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HFO4309643.1"
                     /translation="MNELKKRLQYVSPRERRLLIGCVALLVVVFVYYVLWQPWLIRED
                     KWRTVVTREKNTVEWMKQQVPNIKRREASMPEQGEPLGLSAAVTRTSAAYGVSVTRLQ
                     PQGERLAVTLAPVEFNALMQWLTQLERRHRIRTMVFDVAAQGNPPGQVTVNRLVLSQD
                     DVNKDASLSR"
     gene            13465..14235
                     /locus_tag="ACHJWA_003095"
     CDS             13465..14235
                     /locus_tag="ACHJWA_003095"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFO4309644.1"
                     /translation="MGGVTGSFLNVVIHRLPIMLERRWRREALTLLAHSVPESGPAFN
                     LMIPQSHCPCCFHPLGIRDNIPLLSYLFLKGKSRCCGERIPVYYPLVEITNSVLFILA
                     ASRFPPGLTLAAAWLFISMLLVLAVIDCHTALLPDVLTLPLLWLGLLFSLQRGVVTLE
                     EAVVGAVSGYLCLWGLYWLFRFATGREGLGYGDFKLAAALGAWMGWQALPSILLFASV
                     SGLIVTVLLRIVTATDFTRPLPFGPWLALSGVCHFLLM"
     gene            14327..14728
                     /gene="eptO"
                     /locus_tag="ACHJWA_003096"
                     /gene_synonym="gspS"
     CDS             14327..14728
                     /gene="eptO"
                     /locus_tag="ACHJWA_003096"
                     /gene_synonym="gspS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspS family T2SS pilot lipoprotein variant EptO"
                     /protein_id="HFO4309645.1"
                     /translation="MMGNILKKLNCIASLLVLVTISGCHQSPSIHKQATVPPSEQLEQ
                     MASIVSATRYLKMRCNRSDLPDEQSILNVANRIAIGKGWQSLTQEDIRKHSDDIYVRL
                     TRDSTPEYIKCREFNRRLVPFIGELLARGRG"
     gene            14907..16078
                     /locus_tag="ACHJWA_003097"
     CDS             join(14907..15156,15156..16078)
                     /locus_tag="ACHJWA_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611522.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO4309646.1"
                     /translation="MKKRNFSAEFKRESAQLVVDQNYTVADAAKAMDIGLSTMTRWVK
                     QLRDERQGKTPKASPITPEQIEIRELRKKLQRIEMENEIFKKGYNRALDVRLPEQFSI
                     IGKLRAHYPVVTLCHVFGVHRSSYRYWKNRPEKPDGRRAVLRSQVLELHNISHGSAGA
                     RSIATMATLRGFRMGRWLAGRLMKELGLVSCQQPAHRYKRGGREHVTIPNHLGRQFAV
                     TEPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTIKALKMAWEI
                     RSKPAGVMFHSDQGSHYTSRQFRQLLWRYQIKQSLSRRGNCWDNSPMERFFRSLKNEW
                     IPVTGYMNFSDAAHEITDYIVGYYNALRPHEYNGGLPPNESENRYWKNSKAVASFC"
     gene            16619..17134
                     /gene="ehxC"
                     /locus_tag="ACHJWA_003098"
                     /gene_synonym="hlyC"
     CDS             16619..17134
                     /gene="ehxC"
                     /locus_tag="ACHJWA_003098"
                     /gene_synonym="hlyC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin-activating
                     lysine-acyltransferase EhxC"
                     /protein_id="HFO4309647.1"
                     /translation="MKSNAFDVMGKVAWLWACSPLHKKWPLSVFAINVIPAIQTNQFA
                     LLIKDELPVAFCSWASLDLECEVKYINDVTSLYAKDWMSGERKWFIDWIAPFGHNMEL
                     YKYMRKKYPYELFRAIRLDESSKTGKIAEFHGGGIDKKLASKIFRQYHHELMSEVKNR
                     QDFNFNIEKEN"
     gene            17136..20132
                     /gene="ehxA"
                     /locus_tag="ACHJWA_003099"
     CDS             17136..20132
                     /gene="ehxA"
                     /locus_tag="ACHJWA_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin EhxA"
                     /protein_id="HFO4309648.1"
                     /translation="MTVNKIKNIFNNATLTTKSAFNTASSSVRSAGKKLILLIPDNYE
                     AQGVGINELVKAADELGIEIHRTERDDTAIANQFFGAAEKVVGLTERGVAIFAPQLDK
                     LLQKYQKVGSKIGGTAENVGNNLGKAGTVLSALQNFTGIALSGMALDELLRKQREGED
                     ISQNDIAKSSIELINQLVDTVSSINSTVDSFSEQLNQLGSFLSSKPRLSSVGGKLQNL
                     PDLGPLGDGLDVVSGILSAVSASFILGNSDAHTGTKAAAGIELTTQVLGNVGKAVSQY
                     ILAQRMAQGLSTTAASAGLITSAVMLAISPLSFLAAADKFERAKQLESYSERFKKLNY
                     EGDALLAAFHKETGAIDAALTTINTVLSSVSAGVSAASSASLIGAPISMLVSALTGTI
                     SGILEASKQAMFEHVAEKFAARINEWEKEHGKNYFENGYDARHAAFLEDSLSLLADFS
                     RQHAVERAVAITQQHWDEKIGELAGITRNADRSQSGKAYINYLENGGLLEAQPKEFTQ
                     QVFDPQKGTIDLSTGNVSSVLTFITPTFTPGEEVRERKQSGKYEYMTSLIVNGKDTWS
                     VKGIKNHKGVYDYSKLIQFVEKNNKHYQARIISELGDKDDVVYSGAGSSEVFAGEGYD
                     TVSYNKTDVGKLTIDATGASKPGEYIVSKNMYGDVKVLQEVVKEQEVSVGKRTEKIQY
                     RDFEFRTGGIPYDVIDNLHSVEELIGGKHDDEFKGGKFNDIFHGADGNDYIEGNYGND
                     RLYGDDGDDYISGGQGDDQLFGGSGNDKLSGGDGNNYLTGGSGNDELQAHGAYNILSG
                     GTGDDKLYGGGGIDLLDGGEGNDYLNGGFGNDIYVYGQNYGHHTIADEGGKGDRLHLS
                     DISFDDIAFKRVGNDLIMNKAINGVLSFNESNDVNGITFKNWFAKDASGADNHLVEVI
                     TDKDGREIKVDKIPHNNNERSGYIKASNIASEKNMVNITSVANDINKIISSVSGFDSG
                     DERLASLYNLSLHQNNTHSTTLTTTV"
     gene            20182..22302
                     /gene="ehxB"
                     /locus_tag="ACHJWA_003100"
                     /gene_synonym="hlyB"
     CDS             20182..22302
                     /gene="ehxB"
                     /locus_tag="ACHJWA_003100"
                     /gene_synonym="hlyB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter
                     permease/ATPase EhxB"
                     /protein_id="HFO4309649.1"
                     /translation="MMSKCSSHNSLYALILLAQYHNITVNAETIRHQYNTHTQDFGVT
                     EWLLAAKSIGLKAKYVEKHFSRLSIISLPALIWRDDGKHYILSRITKDSSRYLVYDPE
                     QHQSLTFSRDEFEKLYQGKVILVTSRATVVGELAKFDFSWFIPSVVKYRRILLEVLTV
                     SAFIQFLALITPLFFQVVMDKVLVHRGFSTLNIITIAFIIVILFEVILTGARTYIFSH
                     TTSRIDVELGAKLFRHLLALPVSYFENRRVGETVARVRELEQIRNFLTGQALTSVLDL
                     FFSVIFFCVMWYYSPQLTLVILLSLPCYVIWSLFISPLLRRRLDDKFLRNAENQAFLV
                     ETVTAINTIKSMAVSPQMIATWDKQLAGYVASSFRVNLVAMTGQQGIQLIQKSVMVIS
                     LWMGAHLVISGEISIGQLIAFNMLAGQVIAPVIRLAHLWQDFQQVGISVERLGDVLNT
                     PVEKKSGRNILPEIQGDIEFKNVRFRYSSDGNVILNNINLYISKGDVIGIVGRSGSGK
                     STLTKLLQRFYIPETGQILIDGHDLSLADPEWLRRQIGVVLQENILLNRSIIDNITLA
                     SPAVSMEQAIEAARLAGAHDFIRELKEGYNTIVGEQGVGLSGGQRQRIAIARALVTNP
                     RILIFDEATSALDYESENIIMKNMSRICKNRTVIIIAHRLSTVKNANRIIVMDNGFIS
                     EDGTHKELISKKDSLYAYLYQLQA"
     gene            22306..23745
                     /gene="ehxD"
                     /locus_tag="ACHJWA_003101"
     CDS             22306..23745
                     /gene="ehxD"
                     /locus_tag="ACHJWA_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter subunit
                     EhxD"
                     /protein_id="HFO4309650.1"
                     /translation="MRFYMKGLWDLVCRYKTVFSDVWKIRHTLDAPVREKDEYAFLPA
                     HLELIETPVSRRSHFVVWSILLFVIISLLLSVLGKVEVVSVANGKFTHSGRSKEIKPI
                     ENAIVEKIMVKDGSFVKKNDPLVELTVPGVESDILKSEASLLYEKTEQYRYAILSESI
                     QRNELPEIRITDFPGGEDNAGGEHFQRVSSLIKEQFMTWQNRKNQKQLTLNKKIVERD
                     AALARVSLYEHQVSQEGRKLNDFKYLLNKKAVSQHSVMEQENSYIQAKNEHAVWLAQV
                     SQLEKEIELVREELALETNIFRSEIIEKHRKSTDNIVLLEHELEKNRQRKASSFIKAP
                     VSGTVQELNIHTEGGVVTTAETLMIIVPDNDILEVTASVLNKDIGFIQPGQEVVIKVD
                     AYPYTRHGYLTGKVKNITADSVSVPDTGLVFNVIISVDRNDIQGERKKIPVTAGMTVM
                     AEIKTGVRSVISYLLSPLKETINESLRER"
     gene            23812..24006
                     /locus_tag="ACHJWA_003102"
     CDS             23812..24006
                     /locus_tag="ACHJWA_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO4309651.1"
                     /translation="MLFKQTMIQFLSLKIVNGMDSIKLLDKVIIYQQQNEQLYPAQEH
                     LLLQLCMRVTKKLTDILMPP"
     gene            24036..24320
                     /locus_tag="ACHJWA_003103"
     CDS             24036..24320
                     /locus_tag="ACHJWA_003103"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFO4309652.1"
                     /translation="MLLALLSSTDNFCLSSTELSERLDVSRTYITRACDSLEKFGFIK
                     RMESKEDRRSKNIYLTSDGNLYLQRTTRIYGRYLKKYGPTLQMMKSKHLK"
BASE COUNT         6655 a         4804 c         6370 g         6513 t
ORIGIN      
        1 ttttttattg agttttgttt tgtatattaa atatttgttg ttgattcatt atcacacaaa
       61 agaatgtatc ataatgcaat tgtttgatgt gttaaacgtt ataaacatat taattgtcgt
      121 gttttgtgct tgcttatcaa aaggatagtt agcttgcttt ttttgcaaaa tataataata
      181 aaataagtta aaggacgtta taagagttaa ttctttggtc aggatatatt tacgaaacag
      241 gtgtaaatat gttataaaaa taaccaacga ctagtgaata agtcgctcct gaaaaaataa
      301 aatatagaaa tactgttata tccggctgca tgaacactaa aatgaatgag agatggagaa
      361 caccgatgaa attaaagtat ctgtcatgta cgatccttgc ccctctggcg attggggtat
      421 tttctgcaac agctgctgat aataattcag ccatttattt caatacctcc cagcctataa
      481 atgatctgca gggttcgttg gccgcagagg tgaaatttgc acaaagccag attttacccg
      541 cccatcctaa agaaggggat agtcaaccac atctgaccag cctgcggaaa agtctgctgc
      601 ttgtccgtcc ggtgaaagct gatgataaaa cacctgttca ggtggaagcc cgcgatgata
      661 ataataaaat tctcggtacg ttaacccttt atcctccttc atcactaccg gatacaatct
      721 accatctgga tggtgttccg gaaggtggta tcgatttcac acctcataat ggaacgaaaa
      781 agatcattaa tacggtggct gaagtaaaca aactcagtga tgccagcggg agttctattc
      841 atagccatct aacaaataat gcactggtgg agatccatac tgcaaatggt cgttgggtaa
      901 gagacattta tctgccgcag ggacccgacc ttgaaggtaa gatggttcgc tttgtttcgt
      961 ctgcaggcta tagttcaacg gttttttatg gtgatcgaaa agtcacactc tcggtgggta
     1021 acactcttct gttcaaatat gtaaatggtc agtggttccg ctccggtgaa ctggagaata
     1081 atcgaatcac ttatgctcag catatttgga gtgctgaact gcctgcgcac tggatcgtgc
     1141 ctggtttaaa cttggtgatt aaacagggca atctgagcgg tcgcctaaat gatatcaaga
     1201 ttggagcacc gggtgagctg ttgttgcata caattgatat cgggatgttg accactcccc
     1261 gggatcgctt tgattttgcc aaagacaaag aagcacatag ggaatatttc cagaccattc
     1321 ctgtaagtcg tatgattgtt aataattatg cgcctctaca cctaaaggaa gttatgttac
     1381 caaccggaga gttattgaca gatatggatc caggaaatgg tgggtggcat agtggtacaa
     1441 tgcgtcaaag aataggtaaa gaattggttt cgcatggcat tgataatgct aactatggtt
     1501 taaatagtac cgcaggctta ggggagaata gtcatccata tgtagttgcg caattagcgg
     1561 cacataatag ccgcggtaat tatgctaatg gcatccaggt tcatggtggc tccggaggtg
     1621 ggggaattgt tactttagat tccacattgg ggaatgagtt cagtcatgaa gttggtcata
     1681 attatggtct tggtcattat gtagatggtt tcaagggttc tgtacatcgt agtgcagaaa
     1741 ataacaactc aacttgggga tgggatggtg ataaaaaacg gtttattcct aacttttatc
     1801 cgtctcaaac aaatgaaaag agttgtctga ataatcagtg tcaagaaccg tttgatggac
     1861 acaaatttgg ttttgacgcc atggcgggag gcagcccttt ctctgctgca aaccgtttca
     1921 caatgtatac tccgaattca tcggctatca tccagcgttt ttttgaaaat aaagctgtgt
     1981 tcgatagccg ttcctccacc ggcttcagca agtggaatgc agatacgcag gaaatggaac
     2041 cgtatgaaca caccattgac cgtgcggagc agattacggc ttcagtcaat gagctaagtg
     2101 aaagcaaaat ggctgagctg atggcagagt acgctgtcgt caaagtgcat atgtggaacg
     2161 gtaactggac aagaaacatc tatatcccta cagcctccgc agataataga ggcagtatcc
     2221 tgaccatcaa ccatgaggcc ggttataata gttatctgtt tataaatggt gacgaaaagg
     2281 tcgtttccca ggggtataaa aagagctttg tttccgatgg tcagttctgg aaagaacgtg
     2341 atgtggttga tactcgtgaa gcgcgtaagc cagagcagtt tggtgttcct gtgacgaccc
     2401 tggtggggta ttacgatccg gaaggcacgc tgtcaagcta catctatcct gcgatgtatg
     2461 gtgcctatgg cttcacttat tccgatgata gtcagaatct atccgataac gactgccagc
     2521 tgcaggtgga tacgaaagaa gggcagttgc gattcagact ggctaatcac cgggctaaca
     2581 acactgtaat gaataagttc catattaacg tgccaacaga aagtcagccc acacaggcca
     2641 cattggtttg caataacaag atactggata ccaaatcgct cacacctgcg ccagaaggac
     2701 ttacctatac tgtaaatggg caggcacttc cagcaaaaga aaacgaggga tgcatcgtgt
     2761 ccgtgaattc aggtaaacgt tactgtttgc cggttggtca acggtcagga tatagccttc
     2821 ctgactggat tgttgggcag gaagtctatg tcgacagcgg ggctaaagcg aaagtgctgc
     2881 tttctgactg ggataacctg tcctataaca ggattggtga gtttgtaggt aatgtgaacc
     2941 cagctgatat gaaaaaagtt aaagcctgga acggacagta tttggacttc agtaaaccta
     3001 ggtcaatgag ggttgtatat aaataatcct gagacggtgc ggccacttcg gccgcattag
     3061 taacacattc gtcccgttaa tcaaaacata aagtagcgtc cagcgtttta ttatgttgtt
     3121 ctttctatca ttccggggtg accggggctt atttataaaa gatatcgtac ttaaaatgct
     3181 gacgccaaac cggctattgt gcgtaatttt acttattgcc ggatatcagc tggtgtcggt
     3241 tatccatcat ttctggctga ctcaggcagc atcggtgccc ggcttatccc gtgttagcgc
     3301 gccggaaaca gcggtaactg gtgatcaaac tgaagaacgt tttgttttta cgttattcgg
     3361 cagggcatcc ccactatcat cggaggggag agcgcaggaa acaatgcctt ccctgtcaga
     3421 tgatctgctt tcaggggagg atcttgacgt gagaggtata ctttatagtt cggttgcaga
     3481 gcattccgtt gccatatttg cacataataa cagacagttc agtctgagcg tcggcgaaaa
     3541 agtacccagc tacgatgcta cgattagtgc tatttttagc gatcatattg ttattaacta
     3601 tcagggaaaa actgtatcac tgcctctgcg atatgataat accgaaaaaa agaatgcata
     3661 tgacaataat aatttaacag ttggagacgt gataacccaa gataattttc gggtagaaag
     3721 tgtttttgat attatgagct tttcagccgt tacggttaat aatacattaa gcggttatcg
     3781 cttgattccg ggtaagcaca gttcgttatt ttataatgct gggttgcatg ataacgatct
     3841 ggccgtatcg gttaatggtt cagaattgcg tgataccaga caggcgcagc agataatgaa
     3901 gcaattgcca gaacttaaag aaataaaaat aaccgtcgag cgtgatggtc agttatatga
     3961 tgcatttatt gctgtaggag aaaactgatt gttcaggaaa tggttgaatg ggcgtttgcc
     4021 ggtacttgtg ttcactacag taattttggg ggccattcca gggtgggggg ctgaattttc
     4081 ggccaacttt aaagatacgg atattcagga gttcataaat actgtcagta aaaatttaca
     4141 caaaacggta ataattaatc ctgacgtgca gggaaccatc actgtacgca gctacgatat
     4201 gctgaacgag gaacaatatt atcagttctt tctcagtgtg ctggacgttt atggttttgc
     4261 tgtggtcgat atgcacaacg gtatactgaa agtagtgcgc tcaaaagatg ccaaaacgtc
     4321 ggcggtgccg gtagctagtg atgtcagtcc cgggactggt gatgaggttg ttacccgggt
     4381 ggtccccgta agtaacgtgg cagccagaga tctggcgcct ttgctgcgtc agctcaatga
     4441 taatgctggc gcaggaagcg tggtgcatta tgaaccttct aatgttttgt tgatgaccgg
     4501 acgtgctgca gtgatgaaac ggttgatgga gattgttgaa cgtgtggata aggtgggtaa
     4561 tcgcagcgtt gccacggtcc cgctcaccta cgcgtccgca acagacgtag ccagacttgt
     4621 tacggaactg actaaagaaa cagataagac agctatacct gcttggatga cggcgaaact
     4681 ggttgcagac gagaggacaa actcagtgct cgtcagcgga gagccaatct cccaacagcg
     4741 tatcatctcc ataattaagc aactggatcg tcaggaggat gttcagggta atactaaggt
     4801 gatttacctg aaatatgcga aggcgaagga tttagtggaa gtcctgacag gtatcagcag
     4861 cagtattgaa aacgactcta aaaagagtcc gtcaacggaa gccttgcgca aaggagtgac
     4921 gattaaatcc cacgaacaaa ccaatgccct gatcctgacg ggggcccctg acgtcatccg
     4981 cgaccttgaa aatgtgattt cgcagttgga tattcgtcgt cctcaggtcc tggtggaggc
     5041 catcattgct gaaatacagg atgctgacgg gctgaacctt gggatccagt gggtgaataa
     5101 acatgccggt gtggcgcagt tcaccagtac cggtttacct attaccacga tggttcagac
     5161 tcgtcagaac gaaatcttag acagcgatca gagcaatgcc ctgagcatgt ttaacggaat
     5221 tgcagcgggg ttttatcagg gaaactgggc gatgctgttg acggcgctct ccacaagtag
     5281 caagaatgat atcttggcga cccccagtat tgtcacgctg gacaatatgg aggccacttt
     5341 caatgttggt caggaggtcc cggtactttc gggctcacag acaacctctg gggacaatat
     5401 ttttaacacg gtcgagcgca aaacggtggg gatcaaactc agggtaaaac cccagatcaa
     5461 cgagggtgat tccgtgttac tggagataga acaggaggtg tccggtgtgg cggacactgc
     5521 agtagccacc actactgact tgggagcaac cttcaacacc cgaacagtga ccaatgccat
     5581 gctggtcggg aatggcgaaa cggtggtggt cggaggatta ctggataagt cgatcagggg
     5641 gagtgagagt aaagtgccac tgctggggga tatcccggta ctggggcatc tttttcgcgc
     5701 aaaaagcgaa cagacagcta agcgtaatct gatgctgttc attcggccaa ctattattcg
     5761 tgagcgcgac ggatttcgtc atgcttcggc cgaaaaatac cagtcgttta atcaggaaca
     5821 ggtgcagtcg cgtggcaaag aaacaacggc gctgacgctg aatgaggaac agctcaggct
     5881 gtcccccgat caagacgata cggctttccg gaaggtgaaa gcggcgattg ctgcgtttta
     5941 tgcgcaggag atgtaatgag cagagtagta cagaatgtca gtgaatcacg tccccttctg
     6001 cctttttcct tttcccgtac gcagagaatt ttgttgctgc gtgaacagga ggggaatcga
     6061 gtgttctgca tggaggacac acctgccagt gccctgttgg aggtgcgcag ggtggctgaa
     6121 ggtcctctga atgtcacaac agtgtcggcg gaagcgtttg aaaaacagct ggtgagcagc
     6181 tatcaacgtg attctgatga agcgcgacaa atgatggctg agattggtaa tgaaatggat
     6241 ttttataccg tcgcagggga gttgcctgac cgtgaggact tactggatgc gaatgacgat
     6301 gcgccaatta tccgcctgat taacgcaatg ctgacagagg caattaaaga gaaagcctca
     6361 gatattcata ttgaaactta cgaacgccat ctgcaggttc gctttcgcat tgatggcgtt
     6421 ctgcgggaga tcctccgttt acataggaat ctggcttcgt tactgatttc acgcattaaa
     6481 gtcatggccc ggttggatat tgccgaaaaa cgcgttcccc aagatggccg catggtactg
     6541 cgtatcggtg gtcgggcggt ggatgtgcgt gtttcaacgt tgccttcaaa tcatggtgaa
     6601 cgcatcgtgc tgcgtttact agacaaaaat agcgttagtc tcgatcttgc tgcactcggc
     6661 atgtcgcagc agaatcagcg acacattgat gcactgatcc gccgtcctca cggcattatt
     6721 ttggtgaccg ggcctacagg gtcgggtaaa agcacgacgc tttatgccgc gttaagcctg
     6781 ctgaatcccc gagaccgcaa cattatgacg gtcgaggatc ctgttgaata tgaactggac
     6841 ggtatcagtc agacccaggt caacccgaag gtggacatga cctttgcccg gagtctgcgc
     6901 gctattttac gtcaggatcc ggacgtggtg cttgtggggg agatccgtga cggtgaaaca
     6961 gcccagattg ctgtgcaggc ctcgctgaca gggcatttgg tgctgtcaac gttacatacg
     7021 aatagtgcag caggcgcgct gtcgcgtctg caggatatgg gcatccctcc ttttctgctt
     7081 tccacctcat tactggctgt tctggcccag cgactggttc gcacgttgtg tccgcgctgt
     7141 cgtcagccct gtcaggtcag tactgagtta gcgatggaca tggacattcc ccctgaaacc
     7201 acgatctggc agcctgccgg gtgtcagcac tgcagtttca cgggctatca cgggcgcacc
     7261 gggatccacg agctgttgct gattgacgat cgcattcgga cggcaatcta tcagggggag
     7321 ggggagctgg gtattacccg cttggcaggg agtcgctatc tgacgctgcg tggcgacggg
     7381 cgacaaaagg tactagccgg tgagaccagt tgggaggagg tggttcgcgt tactgaaagc
     7441 agattgcagg aagaggaatg atatggcgtt gtttcattat caggcatcag atattcacgg
     7501 tagaaaacgc agtggcattc tggaggcgga ttcggcccga catgcccgtc agttgctgcg
     7561 tgagcaggcg cttattccag tcaggttgga tgagaaacag gtccatcaca agcactcact
     7621 gcggagtatc ctgaggtttc gtccgcgcgg ggggagcagc gccgaactcg cgttactgac
     7681 acggcagtta gccacactag tggccgcatc tctgccgctt gaagaggcgc tggatgcgtt
     7741 gttgaggcag agtgaaaaac cgcgtcagcg gaacttaatc gctgccgtgc gcaccaaggt
     7801 tctcgaggga cattccttgg ccgcggcgat gggcatgttt ccaggtacgt ttgagcgtct
     7861 ttattgtgcg atggtggcgg cgggggaaac atccggtcgt ttggacgtgg tgctcagtcg
     7921 tctggctgac tataccgagc agcgccagat catgcgaaac cgtctactcc aggcattgct
     7981 ctatccttgt gttctgacgt tggtggcggt tggggttatt gccattctgc ttactgcggt
     8041 tgtgccgaaa gtggttgagc agtttattca tatgaaacag acccttccct tatctacccg
     8101 cgtactgatg ggggctgctg aggtgagcca gacttggggc ccgtggctgt tactggccgc
     8161 agcgctgggc gggatagcgg ggcgcatgat attacatcag ccctcccagc gtctggcttt
     8221 tcatcatctg ctgttgcggc tgccggtcgt ggggcgcata tcgcgcggtc tgaataccgc
     8281 acgttatgcc cgcacgctga gcattcttaa tgccagtgcg gtaccgctgc tccaggcgat
     8341 gcacatcagt ggtgacgtac tgagtaatga ctgggcccgt catcagttag ctaccgcggc
     8401 cgagttggtt agggaagggg tcagcctgca tcaggcactg gagcagactt cgctgtttcc
     8461 gcctatgatg cggcacatga ttgccagcgg tgaaaatagt ggcgaactcg acagcatgtt
     8521 ggaacgggcc gccgacaatc aggatcgtga gttcagcaca cagatgcaac tggcgctggg
     8581 attgtttgaa ccgctgttgg tggtgggtat ggccggggtc gttttgttta ttgttctggc
     8641 aattctgcag ccgctcctgc agctcaacaa catgatgaat atgtgaatcc ttctttaaca
     8701 gtcaacggag aaaaacatgc gaaaacagca tcagcgtggt tttaccttgc tggaaataat
     8761 ggtggtaatt gtgatcttgg gagttttggc cagtctggtg gtcccgaatc tgatgggaaa
     8821 taaggataag gccgatcggc agaaagtgat gagcgacttg gttgcgctgg aaagcacgct
     8881 cgatatgtac cggttagata ataaccggta tccgacaacg gagcagggac tgcgggcact
     8941 ggtcagtaaa cccaccgtgc aacctgaacc ccgtaactac cggcaggatg gttatatccg
     9001 ccgtttgccc caggatcctt ggggggggga ttatcagctg ttaaatcctg gtcagtacag
     9061 cgacattgat atcttttctc ctggcccgga tggtgtgcca aacactgaag atgatattgg
     9121 taactggacg ctcggtaacg cgcagccatg agtcagcgcg gtttcacaat actggagatg
     9181 atgctgatcc ttttgttgat gggcatcact gcagggctgg tactaatgtc atttcctgac
     9241 tctgctcaga atcatctgca acaacagcgg gagcgtctgc aggcgcagct ggattatgcc
     9301 ttggatagaa gccagcagga cggcttgctg atggggattc aggttcggtt tgacggatgg
     9361 aaatttaagg ttctgcagcg cggaacagcg gaaagccccc cgacattagc tgagggaggg
     9421 gatatttggc aggggtatgt ctggcaaact tggcaaccgc gacgtgcagc catggggggc
     9481 aaacttccgg atgatgtgcg tcttgagctg cagtatctca gaggtttgca gtggatgtct
     9541 gagcatgatg acggggctga acccgatatt ttgctgcttc ccggaggtga ggtaacgccg
     9601 ttcagacttc ttttcaggca ggtcggagag gaggccgttg tcgggctgca ggttgatgag
     9661 aacggtttga tgactctttt tgaaggtgag gtctcattgt gaaggggcaa gaaggaatga
     9721 cattactgga agtcatagta gctctggttg tgtttgcgct ggcaggaatg gcgctgatgc
     9781 aggcctcgac ccagcaggct gcggggatcg gacggatgga agagaaggtg ctggcaggct
     9841 ggttggccga taaccagatg gtgcaattgc aactcgagaa aacttggccg gaaaatggct
     9901 ggggcgagaa gacgatcagc ttcgctggca ccgaatggta tttacgctgg ggcgggccgg
     9961 acagcgacgt gccgcagccg cgctccttag aggtagaggt acggcgcact aaagaggaga
    10021 cgtccgcctt ggtttccttg cgtagcagtg tggttcgtga atgagtcagc aacgggtaaa
    10081 aggttttacg ctactggaaa tgctgttggc actggctgtt tttgcagcgt tgagcatcag
    10141 cgcatttcag gtgctgcagg gtggaatacg ggcgcacgag ctgtcccggg ataaagtaca
    10201 gcgtctggct gagctgcaga gagggatcag ccagatggag cgagatctta cgcagatgct
    10261 cccaaggcac agccggggga atgaaggcct gctgctcgct gcacctcatc tgctaaaaag
    10321 tgatgactgg gggatttctt ttacgcgaaa cagctggctg aatcccgccg ggatgctgcc
    10381 gcgcccggag ttacagtggg taggttatcg gttgcgacag caaaagttag aacgcctgag
    10441 ctactttcat gtcgatcacc cttccggtgt ttctccggat gtcagggtga tgctggacgg
    10501 cgttcacgca tttcgtctgc gtttttttgt taacggtgat tggcaggcgc gctgggacag
    10561 taccggcatc ctgccgcagg ctgttgaagt gacgctggtt atggatgatt ttgctgaact
    10621 gccccgtctg tttcttgtga gcaaggagac cgctgaatga aattgcggga acagggcgtg
    10681 gcgcttctgg tggtattgct gatcctgtcg ttaatggtaa cgatagcggc ggtgattgcg
    10741 gagcgcaacg gccggacatt tttgcgtaca gtagcgcagt tggatcagct gcaggcaaag
    10801 tgggatggct atacggcaga gacgatagca aaacaaatct tacagaggag caggcaggag
    10861 tcgccccgga aaacgcatct ggcacaaaat tgggcgcagt cagaacgaca gtttgagacc
    10921 agagggggag atgtgcgggg gcagatcgtg gatgcgcagg cctgttttaa tcttaatgct
    10981 attaattatg gagtggtgga tctgaccagt ataccttatg ccgccagaat ttttcagcag
    11041 ctattaatca acttgcaggt agagctttta caggcccggc aagtgacggc agccctacgt
    11101 gactggatcg atcgtgatga taagccggta aggggcggtg ctgaagacga ggtgtatatg
    11161 gggatggaac ctccctatct ggcagccaac cagccgatgc aggacgtcag tgagttacgc
    11221 cttatccggg gaatagatgc caggctttac cgcaaacttt tgccgtatgt ttgtgtactg
    11281 cctacgtcag atctgtctgt gaatgtgaat actctgctgg attcacaggc gccgctgctg
    11341 gcggcgctct ttctgacgaa accggacagt cttccggtga cggaattact gcagcggcga
    11401 ccccgtactg gttgggagag cgtggcggcg tttcttgacg cgccaccgct taaggacatt
    11461 gacaccagtg cggccatgcc ggtattggcc gtcagcagca actattttct ggttcggtta
    11521 catgtgcgct ccggggaaca cctgttcagt cagcagactt taatgcagtg gagggaggag
    11581 cgtttccgga tcattcagcg acagtatgga ttaacgatga gggaggttcc ctgaaaaaaa
    11641 ataacaaaca tgtcgaggct ggtcggcttt ttctgcgact gccggaggat gactcggagc
    11701 ctttattgtg gaggctgatt gcgccagagg agacaggagc cggcagtgtg gcagtcggag
    11761 aaacggatcc gcagttagtc ggtttgctgt cccgttatcc cgcctgggtg ctggttccgt
    11821 caggcagaat tgccttccat caggtcacgc tgccacaccg cacgtggcgt cagcgtttgc
    11881 aggtgctgcc ctttcttctg gaagagcagt tggcgacaga cattgagcag ctgcacttcg
    11941 ccattcttca tcagtcgggt gacaggtgtg atgtggcggt ggtcgaaaaa gcggttatgc
    12001 atcgctggtt agcgtactgt ggacaacttg gggttcggga ggtgaggttg ctgcctgacg
    12061 tactgatgct gccgttggca actgaaggat ggagtgccgt aaagcttgcc gggcagtggc
    12121 tgtttcgccg ggatcgttac gccgggatga cggtagagtt atcgtggctg acacatttct
    12181 tgtctcttac tgcgccgccg gtgattgagt cctactccgt gccgccagta ccgtgcccgg
    12241 gtccgaagac cactgagtgg cgaaatcagc ccggacgcga tctgctgcag ttggtggccg
    12301 aaggtgacgg gtatgatggc gcagatctgc ggcagggaga atttgcccgc cagggaacat
    12361 ggtatgcacg atttcgccca tggaggtatg tcgcaggggc tctgctggcc tgtgtttttc
    12421 tggccgggag caacgcgggg ctggcacatt atcggctctg gcagcaggct caattctggc
    12481 ggtatgagag cgtacgtgtc tatcagcagc tgtttccgag tgaaaaggcg gtgaaggatc
    12541 cccgccggca gatgctgaga catctgcaac agtcgacggg taacaatata ccggaactcg
    12601 ggagcgtgat gcgccagctt cagcaactgc tgtcagaaac ctcaacgata cgcttacagg
    12661 cactcgcctg ggacagcagc agccagacac tgaaaattga tttgcaggcg gcttcttttc
    12721 aggcgctgga acactttcag cagatcgccg gagaaaaata cctcatccag cctggtgaga
    12781 tacgacagca gccgcagggg gtagaaagtc gtctgatcct gagagtgaac aatgaacgag
    12841 cttaaaaaac gtttgcagta tgttagcccc cgtgagcgca ggctgcttat cggatgtgtt
    12901 gccttactgg ttgttgtttt tgtctattac gtcctctggc agccctggct gatacgagag
    12961 gataagtggc ggactgtagt tacccgtgaa aaaaacacag tggagtggat gaaacaacag
    13021 gtacccaaca taaagagacg ggaggcaagt atgcctgaac agggggagcc gttaggttta
    13081 tctgcggccg tgacacgtac ttcagccgca tacggtgtat cggtcacccg gttgcagcct
    13141 caaggggagc gtctggccgt caccttagcc ccggttgaat ttaatgcgct gatgcagtgg
    13201 cttacgcaac tggagcgtcg gcaccgtatt cgtaccatgg tatttgatgt ggccgcgcag
    13261 ggcaacccac ccggacaggt aacggttaat cggctggttt tgtcccaaga tgacgtaaac
    13321 aaggacgctt cgcttagtcg ctgatgtcgt aatttacaga ttaatgcttg atgtaaaaat
    13381 tggacttata aaggaggatg tgtgagtagt ttgccggaat ttgcggttga ttatcccttg
    13441 ttgtggctca tctgcaccgg agggctgggg ggcgttaccg gcagttttct taacgtcgtg
    13501 attcatcgtc tgccgataat gttggagagg cggtggcggc gtgaagcgct gacgctattg
    13561 gcacattctg tgcctgagtc cgggccggcc tttaacctga tgataccgca gtctcattgt
    13621 ccctgctgtt ttcatccgct cggcatcagg gataacattc ccctgttaag ctacctattt
    13681 ctgaagggga aatcacgctg ttgtggcgag cgtattcccg tttactaccc gctagtcgaa
    13741 ataacgaact cagtgttatt cattctggcc gcgagcaggt ttccaccagg gctgacatta
    13801 gcggctgcct ggctttttat ttccatgctg ttggttctgg cggttattga ctgccatacg
    13861 gcgctgctgc cggatgtgct gacgttgcca ttgctctggc ttggcctgtt attcagttta
    13921 cagagaggcg ttgtcacctt agaggaggcg gtagtggggg cggtatctgg gtatctctgt
    13981 ctgtggggac tgtactggct ttttcgtttc gctaccggga gggaagggtt aggttacggt
    14041 gattttaagc tagctgcggc attgggagca tggatgggtt ggcaggcatt accctcaata
    14101 cttttgtttg cctcagtcag tgggttaatc gtaacagtac tgttgcgtat tgtaacagct
    14161 acggacttta cccgcccgct gccttttggg ccgtggctcg ctttgagcgg agtgtgccat
    14221 tttttgttga tgtagcgttc ggttaccggg gctagtaata aaaacggctc agcggataaa
    14281 agaactgttg taaaccggta tcaggttaaa cgatacctag taaaaaatga tgggtaatat
    14341 tttgaaaaaa ctgaattgta tcgcgtcatt actagtctta gtgactatct ctggttgtca
    14401 tcagtcaccc tccatccata agcaggccac tgttcctccg agcgaacaac tggaacaaat
    14461 ggcgtcgatt gtctctgcga cacggtatct gaaaatgaga tgtaaccgta gtgatttacc
    14521 agatgaacag tcgatcctaa atgtcgctaa ccggatcgct atcgggaaag ggtggcagtc
    14581 attaactcag gaggatatac gtaaacacag cgatgatatc tatgtgcgtc tgactaggga
    14641 cagtacgcct gagtatataa aatgcagaga attcaatcgg cggctggtcc catttatcgg
    14701 tgaattattg gcaaggggtc gtggataata tgaaccgccc cggaaatcct ggagactaaa
    14761 ctccctgaga aagaggtaaa cagcatgaat aaaaatgggt cttcccttgt agtggtggct
    14821 gaaggcatga tgattgtagt ggcacactga atttggccac ctgaacagag gtgatatgct
    14881 cacctcagaa caacacaggt gctccaatga aaaaaagaaa tttcagcgca gagtttaaac
    14941 gcgaatccgc tcaactggtt gttgaccaga actacacggt ggcagatgcc gccaaagcta
    15001 tggatatcgg cctttccaca atgacaagat gggtcaaaca actgcgtgat gagcgtcagg
    15061 gcaaaacacc aaaagcctct ccgataacac cagaacaaat cgaaatacgt gagctgagga
    15121 aaaagctaca acgcattgaa atggagaatg aaatattaaa aaaggctaca accgcgctct
    15181 tgatgtcaga ctccctgaac agttctcgat aatcgggaaa ctcagagcgc attatcctgt
    15241 ggtcacactc tgccatgtgt tcggggttca tcgcagcagc tacagatact ggaaaaaccg
    15301 tcctgaaaaa ccagacggca gacgggctgt attacgcagt caggtacttg agttgcataa
    15361 catcagccat ggttctgccg gggcaagaag catcgccaca atggcaaccc tgagaggctt
    15421 cagaatgggg cgctggcttg ccggcaggct catgaaagaa ctgggactgg tcagttgcca
    15481 gcagcctgcg caccgttata aacgaggtgg tcgtgaacat gtcactatcc cgaatcacct
    15541 tgggcggcag ttcgcagtga cagagccaaa tcaggtatgg tgcggcgacg tgacgtacat
    15601 ctggacgggg aaacgttggg cataccttgc cgttgttctc gacctgtttg caaggaaacc
    15661 ggtaggttgg gcaatgtcgt tctctccgga cagcagactg accatcaaag cgctgaaaat
    15721 ggcctgggaa atccgcagta aaccagccgg ggtaatgttc cacagcgatc agggcagcca
    15781 ctatacaagc aggcagttcc ggcagttact gtggcgttac cagatcaaac agagtctgag
    15841 tcgacgagga aattgctggg ataacagccc gatggagcgc ttcttcagga gtctgaaaaa
    15901 cgagtggata ccggtgacgg gttacatgaa cttcagcgat gctgcccatg aaataacgga
    15961 ctatatcgtt gggtattaca acgcgctcag gccgcacgaa tataacggtg ggttgccacc
    16021 aaatgaatcg gaaaaccgat actggaaaaa ctctaaagcg gtggccagtt tttgttgacc
    16081 actacatccc gtcagccgat ttacgggccg taagtaacga agttggatgc aaatgtcaga
    16141 tcgtgtgcgc ctgttatggc tcggctggta agagagcctt ataggcgcat ttgaaaaaac
    16201 tccagctata ccggaagaga tttattatta acaggagagg tttgcttttc tgtgtaggtt
    16261 tagattttgt gtttgcaggg taaacaaaac tgattaactc gtcatttcgg taactgaaat
    16321 agtttgctgt attctgttta caatctcttt aataaggcat ttgtcacgtg gctattcata
    16381 tgaaaatcat acgttatctt acaaatcaat catctgagtg ttataatata acttagctgt
    16441 gatatgtgta agaatgttta ggcaatatat tattatcgct aatgttgttt tggttgggag
    16501 agattctcct tgcggtagct ttctgttttt agatgcttct tgcttaaaag gatataattt
    16561 ctgttctttt atatagggtt ctttacattt actgagtttt cttatttatt taaacattat
    16621 gaaatctaat gcttttgacg tcatggggaa ggttgcatgg ctatgggcct gttctcctct
    16681 gcataaaaaa tggccattat ctgtttttgc tattaatgtc atacctgcta ttcagacaaa
    16741 tcaatttgct ctcttaataa aggatgagct tcctgtagct ttttgtagct gggccagttt
    16801 agatctggaa tgtgaggtaa aatatataaa tgatgtaaca tctttgtatg caaaagactg
    16861 gatgtcggga gaaagaaagt ggtttattga ctggatagct ccatttggac acaacatgga
    16921 gttatataaa tacatgagga aaaagtatcc ttatgagtta ttcagagcaa taaggttaga
    16981 tgagagcagt aaaacaggaa aaatagctga atttcatgga ggggggatag ataaaaaact
    17041 ggcatcaaaa atattcaggc aataccatca tgaacttatg tctgaagtaa aaaacagaca
    17101 agattttaat tttaatattg agaaagaaaa ctaatatgac agtaaataaa ataaagaaca
    17161 ttttcaataa tgcgacattg actacaaaat cagcatttaa tacagcatca tcaagcgtac
    17221 gttccgctgg aaaaaaactc atattattaa tacctgataa ttatgaagct cagggcgtgg
    17281 gtattaatga gttggtcaaa gctgctgatg agcttggaat agaaatacac cgtactgaac
    17341 gagatgatac agcgattgca aaccagtttt ttggtgcagc agaaaaagtt gtaggattaa
    17401 ctgaacgtgg tgttgcaata ttcgcaccac aacttgacaa acttctgcag aagtatcaga
    17461 aagttgggag taaaatagga ggaaccgctg aaaatgtagg taataatctg ggaaaagccg
    17521 gaacagttct ctcagcacta cagaatttta cggggattgc tttatcaggc atggctcttg
    17581 atgaattgct gagaaaacaa cgggaaggag aggatataag tcagaatgat attgccaaaa
    17641 gtagtattga acttattaat cagcttgtag atacagtatc aagtataaac agtaccgttg
    17701 attcattttc tgagcagctt aaccagcttg gctcattttt atccagtaaa cctcgattaa
    17761 gttctgttgg tgggaaatta caaaatttac cagacctggg ccccctgggg gatgggctgg
    17821 atgttgtctc cggaattctt tctgctgtat cagcaagctt tattctggga aacagtgacg
    17881 cacatacagg aacaaaagct gcagcgggta tcgaactgac aactcaggtt cttggaaatg
    17941 ttggtaaagc tgtttcgcaa tatattctgg ctcagagaat ggcacagggg ttatcgacaa
    18001 cagctgcaag tgcgggtctg atcacatcgg ctgttatgct ggctatcagt cctctttctt
    18061 tcctggctgc tgcagataaa tttgagcgag ctaagcagct tgaatcatat tctgaacgat
    18121 ttaaaaaatt gaattatgaa ggggatgctt tactcgcagc ctttcataaa gaaaccggag
    18181 ctatagatgc agccctgaca acaataaata ctgtcctgag ttctgtatct gcgggagtta
    18241 gtgcagcctc cagtgcatcc ctcatagggg ccccgataag catgctggtg agtgcattaa
    18301 ccggtacgat atctggcatt ctggaagcat caaaacaggc tatgtttgag cacgttgcag
    18361 agaaattcgc tgctcggatc aatgaatggg aaaaggagca tggcaaaaat tattttgaga
    18421 atggatatga cgcaagacat gctgcgtttt tagaagactc tctgtctttg cttgctgatt
    18481 tttctcgtca gcatgcagta gaaagagcag tcgcaataac ccagcaacat tgggatgaga
    18541 agatcggtga acttgcaggc ataacccgta atgctgatcg cagtcagagt ggtaaggcat
    18601 atattaatta tctggaaaat ggagggcttt tagaggctca accgaaggag tttacacaac
    18661 aagtgtttga tcctcaaaaa gggaccatag acctttcaac aggtaatgta tcaagtgttt
    18721 tgacatttat aacaccaaca tttaccccag gagaagaagt tagagaaaga aaacagagtg
    18781 gtaaatatga atatatgaca tctcttattg taaatggtaa ggatacatgg tctgtaaaag
    18841 gcataaaaaa tcataaaggt gtatatgatt attcaaaatt gattcagttt gttgaaaaga
    18901 ataacaaaca ctatcaggcg agaataattt ctgagctcgg agataaagac gatgtggttt
    18961 attctggagc aggctcatca gaagtatttg ctggtgaagg ttatgatacc gtatcttata
    19021 ataagacgga tgttggtaaa ctaacaattg atgcaacagg agcatcaaaa cctggtgagt
    19081 atatagtttc aaaaaatatg tatggtgacg tgaaggtatt gcaggaagtc gttaaggaac
    19141 aggaggtgtc agtagggaag cgaacagaga aaatacaata tcgtgatttt gaattcagaa
    19201 ccggtggaat tccttatgat gtaatagata atcttcattc tgttgaagag ctcattggcg
    19261 gaaaacatga tgatgaattc aaaggcggta agtttaatga tatattccat ggcgcagatg
    19321 ggaacgatta tatcgaaggt aattatggta atgatcgact atacggcgat gatggggatg
    19381 attatatatc cggaggacag ggagacgacc agttatttgg tggtagtgga aacgataaat
    19441 tgagtggagg ggatggtaat aattatctga caggaggaag cggtaatgat gagcttcagg
    19501 cacacggagc ttataatatt ctgtcaggtg gtactggtga tgataaactt tatggtggtg
    19561 gtggtattga tcttctggat ggaggggaag gtaatgacta tctgaatggt ggttttggta
    19621 atgatattta tgtttatggg caaaactatg gtcatcatac aattgcagat gaaggaggta
    19681 aaggagatcg tttgcactta tctgatatta gctttgatga tatcgcattt aagagagttg
    19741 gaaatgatct tatcatgaat aaagccatta atggtgtact ttcatttaat gagtcaaatg
    19801 atgtcaatgg gataacattt aaaaactggt ttgcgaaaga tgcctcagga gcagataatc
    19861 atcttgttga ggttataaca gataaagatg gtcgagagat aaaagttgat aagatacctc
    19921 ataataataa tgaacggtca ggttatataa aagccagtaa tatagcatct gaaaaaaaca
    19981 tggttaatat caccagtgtt gccaatgata ttaataagat tatttcttca gtttcagggt
    20041 tcgattcagg tgatgaacga ttagcatctt tatataattt atccttacat caaaacaaca
    20101 cacactcaac aactttaacg acaactgtct gagactaaaa gaggaggtga tggccattcc
    20161 aggaggaatg gtcatcgtta tatgatgagt aaatgtagtt ctcataatag tctgtatgca
    20221 ctgatattgc ttgcacaata tcataatata actgtcaatg ctgaaactat aaggcatcag
    20281 tataataccc acacacaaga ttttggggtg actgaatggt tactggcagc gaaatctatt
    20341 ggcttaaaag caaaatatgt agaaaaacat ttttccagat tgtcaataat ttctttacct
    20401 gcgttgatat ggcgggatga cggtaagcat tatatattgt ctcgtattac taaagattca
    20461 tcacgctatc ttgtttatga tccagaacaa catcagtcac taacttttag tcgggatgag
    20521 tttgaaaaac tgtatcaggg aaaagtcatt ctggttacgt caagagcaac agtagtcgga
    20581 gagttagcta aatttgattt ttcttggttt atcccctctg ttgtgaaata caggaggatt
    20641 ttacttgagg tgttaactgt ttctgctttt attcagtttc ttgcgttaat aacacctctt
    20701 ttttttcagg ttgtaatgga taaggtttta gttcaccggg ggttttcaac gttaaatatt
    20761 atcacaatag catttattat agtgatactt tttgaagtga tattaaccgg agccagaact
    20821 tatattttct ctcatactac aagtcgtatt gacgtcgaac tgggtgctaa gttattcaga
    20881 catttgcttg cattgcctgt ttcatatttt gaaaatcgca gggtcggaga gaccgttgcc
    20941 agagtaaggg aactggagca aattcgtaat tttttaaccg gacaagcgtt gacatcagtt
    21001 cttgatctat ttttttctgt aatatttttt tgtgtcatgt ggtattacag ccctcaatta
    21061 acactggtta tattattgtc actaccttgt tatgttatat ggtcattgtt tatatcaccc
    21121 ttattacgtc gacgtcttga tgataagttt ctcaggaatg cagaaaatca agcttttctt
    21181 gtcgaaacgg taacagcaat aaatacaatc aaatccatgg cagtatcacc acagatgatt
    21241 gctacatggg ataaacaact ggccgggtat gttgcttcca gtttcagggt aaatctggtt
    21301 gcaatgacag ggcagcaggg gatacagctg atacagaaaa gtgtaatggt aattagtcta
    21361 tggatgggag cacatcttgt catatcggga gagataagca tcgggcagtt aatcgcgttt
    21421 aatatgcttg caggtcaggt cattgctcct gttatcagac tggctcatct ttggcaggat
    21481 tttcagcagg ttggtatttc tgttgagcgc cttggtgatg tattaaatac accggtagag
    21541 aaaaagtcag gcagaaatat actgccggaa attcaggggg atatcgaatt taaaaatgtc
    21601 agatttcggt attcttctga cggtaatgtt attttgaata atattaattt atacatatca
    21661 aagggggatg ttatcggtat agttggccgt tctggttcag gaaaaagtac attaacaaaa
    21721 ctgcttcagc gcttttatat accagagacc ggacagattt taattgatgg gcatgattta
    21781 tcacttgcag atccagaatg gttacgacgc cagattggtg ttgtattgca ggaaaatata
    21841 ctattaaatc gtagtattat cgataatatt acattagctt ctccggctgt atctatggaa
    21901 caggctattg aggcagccag acttgcaggt gcccatgatt ttattagaga actaaaagaa
    21961 gggtacaata ctattgttgg agaacagggc gttggtcttt caggagggca gcgccaacgg
    22021 atcgcaatag cccgggctct tgttacaaat cctcgaattc ttatttttga tgaagcaacc
    22081 agtgctcttg attatgagtc ggaaaatata ataatgaaaa atatgtcaag aatatgtaag
    22141 aacagaaccg taattattat tgcacacagg ttgtcaactg taaaaaatgc aaacagaata
    22201 attgttatgg ataatggctt tatttctgaa gatggcacac ataaagagct tatctccaaa
    22261 aaagacagtt tatatgcata tttatatcag ttgcaggcat aacgtatgcg cttttacatg
    22321 aaaggtttat gggatttggt ttgccgttat aagacggttt tttctgatgt ctggaaaatt
    22381 cgtcacacac ttgatgctcc tgtgagagaa aaagatgagt atgcatttct tcctgctcac
    22441 cttgaactca ttgaaacacc agtatccaga cgttctcatt ttgttgtatg gagtatttta
    22501 ttatttgtaa ttatatctct tctcttatct gttcttggga aagttgaagt ggtttcagta
    22561 gcaaatggta agtttactca tagtggaaga agcaaagaaa taaaaccgat agagaatgcg
    22621 attgtagaaa aaataatggt aaaagatggc tcttttgtaa aaaaaaatga tccattagtt
    22681 gaattgacgg tgcctggtgt tgaatctgat attttaaaat cagaagcatc tttgttgtat
    22741 gaaaaaacag aacaatatcg ctacgcaatt ctttctgaat caatacaacg gaatgagctt
    22801 cctgaaataa ggataacaga ttttcctggc ggagaagata atgcgggagg tgaacatttt
    22861 cagagggtta gctcattaat aaaagagcaa ttcatgacat ggcagaacag aaaaaatcaa
    22921 aagcagctca cattaaataa aaaaatagtt gaacgggatg cagcgcttgc tcgtgttagc
    22981 ctgtatgagc atcaggtatc acaggaagga agaaaactca atgattttaa gtatttgttg
    23041 aataaaaaag ctgtttctca acattcagtt atggagcagg aaaatagcta tattcaggca
    23101 aaaaatgaac atgcagtctg gcttgcacag gtttctcaac ttgaaaaaga aatagaactt
    23161 gtgcgggaag aactggcact ggagacgaat atcttcagaa gtgaaattat cgagaagcac
    23221 agaaaatcaa cagataacat tgtgttgctg gagcatgagc ttgaaaaaaa caggcagaga
    23281 aaagcatcat cttttattaa agctcctgtg agtggtactg ttcaggagtt aaatatacat
    23341 acagaaggtg gtgttgtcac aacagcagaa acgctgatga ttattgtccc tgataatgat
    23401 attctcgaag taacagcatc tgtacttaac aaggatatcg gttttataca acctggacag
    23461 gaggtcgtta ttaaagtaga tgcatatcca tatacacgtc atggttacct tacagggaaa
    23521 gtaaaaaata taactgcaga ttctgtttct gttccggata cagggcttgt atttaacgtg
    23581 attatatctg ttgatcggaa tgatatacag ggagaaagaa aaaaaatccc tgttacagct
    23641 ggaatgactg ttatggcaga aataaaaacc ggggtgcgta gtgttatcag ctatcttctt
    23701 agtccattaa aggaaactat taatgaaagt ttacgtgaac gttagtatat aaataacata
    23761 ttggcttttt atatgatata gatttctgaa ataatcttgt aaagattatc tatgttattt
    23821 aaacaaacta tgatacagtt tttatcttta aaaatagtta atggaatgga tagtattaaa
    23881 ttacttgata aagttattat ctatcaacaa cagaatgagc aattatatcc agcacaggaa
    23941 catcttcttt tacaactttg tatgcgagtt accaaaaaac ttactgatat attaatgcct
    24001 ccctgaaggg ggatggtata aacgatacaa cgcttatgct tctagccttg ttatccagta
    24061 ctgataattt ctgcttatct tcgactgaac tcagtgaaag attagatgtt tccagaacat
    24121 atatcaccag agcttgtgat tctctggaaa aatttggttt tattaaacgt atggaaagta
    24181 aggaagatcg acgtagtaag aatatttatc tgacttcaga cggtaatttg tatttacagc
    24241 ggaccacaag gatatatgga cgctatttaa aaaaatatgg accaacctta cagatgatga
    24301 aatcaaaaca tttaaaataa tcagcaaaaa ggttttatct ca
//
LOCUS       DAZFAB010000070        23865 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZFAB010000070 DAZFAB010000000
VERSION     DAZFAB010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23865
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.070"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(76..1341)
                     /locus_tag="ACHJWA_003104"
     CDS             complement(76..1341)
                     /locus_tag="ACHJWA_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4309653.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALVVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGS
                     YKGQPVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQDVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANRERFISPFEYAKINNPLKQ"
     gene            complement(1539..1614)
                     /locus_tag="ACHJWA_003105"
     tRNA            complement(1539..1614)
                     /locus_tag="ACHJWA_003105"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1579..1581),aa:Phe,seq:gaa)
     gene            complement(1720..2418)
                     /gene="yqgA"
                     /locus_tag="ACHJWA_003106"
     CDS             complement(1720..2418)
                     /gene="yqgA"
                     /locus_tag="ACHJWA_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFO4309654.1"
                     /translation="MVIGPFINASAILLGGMLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWFA"
     gene            2571..2654
                     /locus_tag="ACHJWA_003107"
     CDS             2571..2654
                     /locus_tag="ACHJWA_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4309655.1"
                     /translation="MHAISLHSLAYRRFARNPSLFMLLLMR"
     gene            2816..4951
                     /gene="speC"
                     /locus_tag="ACHJWA_003108"
     CDS             2816..4951
                     /gene="speC"
                     /locus_tag="ACHJWA_003108"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFO4309656.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILTLLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENIFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGIDAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGDVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(5001..6257)
                     /gene="nupG"
                     /locus_tag="ACHJWA_003109"
     CDS             complement(5001..6257)
                     /gene="nupG"
                     /locus_tag="ACHJWA_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFO4309657.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(6459..7538)
                     /gene="mltC"
                     /locus_tag="ACHJWA_003110"
     CDS             complement(6459..7538)
                     /gene="mltC"
                     /locus_tag="ACHJWA_003110"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFO4309658.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGGTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(7603..7878)
                     /gene="yggX"
                     /locus_tag="ACHJWA_003111"
     CDS             complement(7603..7878)
                     /gene="yggX"
                     /locus_tag="ACHJWA_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFO4309659.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(7906..8958)
                     /gene="mutY"
                     /locus_tag="ACHJWA_003112"
     CDS             complement(7906..8958)
                     /gene="mutY"
                     /locus_tag="ACHJWA_003112"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFO4309660.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            9119..9838
                     /gene="trmB"
                     /locus_tag="ACHJWA_003113"
     CDS             9119..9838
                     /gene="trmB"
                     /locus_tag="ACHJWA_003113"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFO4309661.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMIEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            9838..10164
                     /gene="yggL"
                     /locus_tag="ACHJWA_003114"
     CDS             9838..10164
                     /gene="yggL"
                     /locus_tag="ACHJWA_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFO4309662.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERNLDEVRTSEL
                     FDVWWD"
     gene            10348..11067
                     /gene="yggN"
                     /locus_tag="ACHJWA_003115"
     CDS             10348..11067
                     /gene="yggN"
                     /locus_tag="ACHJWA_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFO4309663.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            11243..12289
                     /gene="ansB"
                     /locus_tag="ACHJWA_003116"
     CDS             11243..12289
                     /gene="ansB"
                     /locus_tag="ACHJWA_003116"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFO4309664.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDDVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFIASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            12406..13413
                     /gene="yggM"
                     /locus_tag="ACHJWA_003117"
     CDS             12406..13413
                     /gene="yggM"
                     /locus_tag="ACHJWA_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFO4309665.1"
                     /translation="MKKQWIVGTALLMLMTGNVWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWTVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLETALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREDAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWITLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(13656..14792)
                     /gene="hemW"
                     /locus_tag="ACHJWA_003118"
     CDS             complement(13656..14792)
                     /gene="hemW"
                     /locus_tag="ACHJWA_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFO4309666.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLDNDVAYAQGREVKTIFIGGGTPSLLSGSAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEPAPRVEFSAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(14785..15378)
                     /gene="rdgB"
                     /locus_tag="ACHJWA_003119"
     CDS             complement(14785..15378)
                     /gene="rdgB"
                     /locus_tag="ACHJWA_003119"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFO4309667.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTELGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            complement(15386..15676)
                     /gene="yggU"
                     /locus_tag="ACHJWA_003120"
     CDS             complement(15386..15676)
                     /gene="yggU"
                     /locus_tag="ACHJWA_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFO4309668.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEVAALIN"
     gene            complement(15673..16239)
                     /gene="yggT"
                     /locus_tag="ACHJWA_003121"
     CDS             complement(15673..16239)
                     /gene="yggT"
                     /locus_tag="ACHJWA_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFO4309669.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            complement(16257..16961)
                     /gene="yggS"
                     /locus_tag="ACHJWA_003122"
     CDS             complement(16257..16961)
                     /gene="yggS"
                     /locus_tag="ACHJWA_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFO4309670.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            16979..17959
                     /gene="yggR"
                     /locus_tag="ACHJWA_003123"
     CDS             16979..17959
                     /gene="yggR"
                     /locus_tag="ACHJWA_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFO4309671.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLTENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGTPPILPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVGEGRL"
     gene            complement(18150..18566)
                     /gene="ruvX"
                     /locus_tag="ACHJWA_003124"
     CDS             complement(18150..18566)
                     /gene="ruvX"
                     /locus_tag="ACHJWA_003124"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFO4309672.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKIDSASAVIILESYFEQGY"
     gene            complement(18566..19129)
                     /gene="yqgE"
                     /locus_tag="ACHJWA_003125"
     CDS             complement(18566..19129)
                     /gene="yqgE"
                     /locus_tag="ACHJWA_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFO4309673.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(19241..20188)
                     /gene="gshB"
                     /locus_tag="ACHJWA_003126"
     CDS             complement(19241..20188)
                     /gene="gshB"
                     /locus_tag="ACHJWA_003126"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFO4309674.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQ"
     gene            complement(20201..20932)
                     /gene="rsmE"
                     /locus_tag="ACHJWA_003127"
     CDS             complement(20201..20932)
                     /gene="rsmE"
                     /locus_tag="ACHJWA_003127"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFO4309675.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(21012..21719)
                     /gene="endA"
                     /locus_tag="ACHJWA_003128"
     CDS             complement(21012..21719)
                     /gene="endA"
                     /locus_tag="ACHJWA_003128"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFO4309676.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDHYNLTLSRQQTQLFNAWDKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(21814..22311)
                     /gene="yggI"
                     /locus_tag="ACHJWA_003129"
     CDS             complement(21814..22311)
                     /gene="yggI"
                     /locus_tag="ACHJWA_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFO4309677.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYSCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            complement(22388..23782)
                     /gene="galP"
                     /locus_tag="ACHJWA_003130"
     CDS             complement(22388..23782)
                     /gene="galP"
                     /locus_tag="ACHJWA_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFO4309678.1"
                     /translation="MPDAKKQGQSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAGLNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
BASE COUNT         5875 a         6006 c         6257 g         5727 t
ORIGIN      
        1 ctaataccgt tttgtctgtt tttttaacaa agaaaataat cctggagtag gttcccgggc
       61 atttgcccgg gatgattact gttttaatgg attattaatc tttgcatatt caaatggact
      121 gataaatctt tctctgtttg catccagaaa atcggcccac cactgtagca tcagtcgccg
      181 ttcttccaga tgttctgctt tatgaatgta agcagcacgt acaccgttac gttcctgatg
      241 gctcatctga cgttcaactg catcgcggga ccacaaacct gattctatta acgcactaca
      301 tgccatcgcc cggaatccat gaccacagac atcctgggtt gtgtcatacc ccataaccct
      361 cagggcttta tttacggtgt tttcactgat tggtttacgt gggtcatgtg ctcccgtaaa
      421 gatcagacca ttttcaccag cccatgtctg aagttctgcc agtatggcta caacctgttt
      481 gctgagtggc acaagatgtg gtgttcgcat cttggatccc cggtgtgaga attttacgcc
      541 tggcagaggt tcgcgttcgg ctggaatagt ccacatggca ttgtcaatat caatttcaga
      601 ccagcgggcg tatctgagtt cactggaacg aataaaaacc agtaaattca gcataacggc
      661 aagctgggtg acaggttgcc ccttgtaact gccaatacgc gacaaaagtt caggcaggcg
      721 agaaagatca agagcggggc gatgctggcg ttttaccgta gtcaatgcgc cagccatatc
      781 cagagcagga ttatatctga tgatgccaga ctgtacggca taacgcatta cggcagatat
      841 tctttgctgc agacgactgg cgatttcata aatttgttta gcttctgcag cacgaacagg
      901 gataagcagg tccggggtat tgagtgttgt gatatcccga ttgccgataa aggggaatac
      961 gtgtgtctcg aggcttttga gtactttatc gctgtgtgat tgtgcccatt gtttattact
     1021 ggcatgccag cgtcgtgcaa tatgttcgaa agagagttgt aaatcaggga ctactttttt
     1081 agctcttttc tgttcgcaag ggtcaacccc ctccgaaatc agttttcggg cctcatcccg
     1141 ttttgtacga gcttcggaga gagatacttc tggatacaca acaagtgcca gagtcttctc
     1201 tttacccaga atacgataac ggagacgcca gtaacgggaa ccattaggat gtaccagcag
     1261 gaacatgcca gccccgtcag tgagtttata agccttgtca gtgggctttg cagcccggat
     1321 ttttgcgtca gtcagtgcca tagttcagcc ttctgaatgc gttaaagagg gtacaaattc
     1381 tgtcgaactg gggtatgccc gcaattatac ccacacaatg aggttgatgt ggggagaatt
     1441 tggttgagtt cggtagaatt gatttggagg aagaacactt aaatcatggt gttctgaaac
     1501 gaaaaacgga cctccgtgga ggtccgttga tatgaatttg gtgcccggac tcggaatcga
     1561 accaaggaca cggggatttt caatcccctg ctctaccgac tgagctatcc gggcaacggg
     1621 gcgcattaaa cctgattcgc ctcgtctcgt caatcaaatt tcttcaattt actgcagact
     1681 gcacaatcta tcatcacttt gccgctattg cacacattgt caggcaaacc aggcggcgga
     1741 aagcggcatt gccagaagga gtgctggaag catgttgacc accgggaaca ttttaatgcc
     1801 acagatgcgt aatccggttg ccagcagcaa taaaccgcct acggcgctga agtctgccat
     1861 catcgacggt gtggtcagcg gtaatatcag cgcggcagcc cacgccagcg ttaactggat
     1921 gatcagtaat gggatactaa tcaccgatac cgcaataccc agcgagcagg cgaagatcat
     1981 cgccgtaaag aaatcaagaa atgacttggc gattaaaata ctcggatcgc cggtcatccc
     2041 ttcgttcatc gccccgaaga tcccggtgcc gctggcgcaa aacaggacaa taatcgcgac
     2101 ataattctga ataaaagatt catgcgctgg cttcttacgt gagtgacgaa acagattttg
     2161 tgctttggcg accgctgtat tgacgccttt ctccagcaga caaatttcgc cgattaatgc
     2221 tccgagtaag gttgctaaaa ccatcgccgg aaggttggcg catttcacca ccagtaaaat
     2281 accaatcccc agcgatgcca aaccaaaaat tgatgtcatg gagacgcgga tacgttccgg
     2341 taagcgttgg ctaagcagtg caccgagcat gccacccagt aatattgcac ttgcgttgat
     2401 aaaagggccg atgaccacaa gagttcctgt taactgtatt gtttgatttt cttattatga
     2461 actttttgcc ttgttgatgg cttgtctggt cgcaaagtcc tggcttgcac gccttagcga
     2521 aaggtgccat gattgcgcga attttctcct ctctgtacgg agtttgcccg atgcacgcca
     2581 tctccttaca ttctctcgct tatcgccgtt tcgcgcgaaa cccttctctt tttatgctac
     2641 tgctcatgcg gtgaagttaa cgcacgctca ctgcaggaca acagtaaaat cagagccttt
     2701 ctgcttttac tgatgtctgg cggtcggagc tggtgaccag tttgacccat atctcatggg
     2761 gcagggtttt acaccttgtc cagtattctt acttccccga aacgggtttg cgcttatgaa
     2821 atcaatgaat attgccgcca gtagtgaact ggtatcccga ctttctactc atcgccgcgt
     2881 ggtggcgttg ggagatactg attttacgga cgtcgcggca gtcgtcatta ccgctgcgga
     2941 tagtcgcagt ggcattctta cgttgcttaa gcgcaccggt tttcatctac cggtgttttt
     3001 gtattccgaa catgctgttg aattacctgc gggcgttacg gcggtaatca acggcaacga
     3061 gcagcagtgg ctggagctgg aatccgcagc ctgtcagtat gaagagaatt tgctgccacc
     3121 gttttatgac acgctgacgc agtacgttga gatgggcaac agcacctttg cttgccctgg
     3181 acatcaacat ggtgcgttct ttaaaaagca tcctgccgga cgccattttt acgatttctt
     3241 tggtgagaac atctttcgcg ccgatatgtg taacgctgac gtaaaattgg gcgatctgct
     3301 tattcatgaa ggatcggcga aagatgcgca gaaattcgca gccaaagtct ttcatgccga
     3361 taaaacctat tttgtgctga acggcacatc ggcagcgaat aaagtggtga cgaatgcgct
     3421 gttaacgcgt ggcgatctgg tgctctttga ccgtaacaac cataagtcga atcatcacgg
     3481 cgcgttgatt caggcggggg cgacgccggt ctatctggaa gcttcacgta acccgtttgg
     3541 tttcattggc ggtattgatg cgcactgttt taatgaagag tatctgcgcc agcaaattcg
     3601 cgacgttgcg ccagaaaaag ccgacctgcc gcgcccgttt cgcctggcga ttattcagct
     3661 gggaacctat gacggcactg tctataacgc ccgtcaggtg atcgataccg ttgggcatct
     3721 gtgtgattac attctgtttg attccgcgtg ggtcggttat gaacaattta tcccgatgat
     3781 ggcggatagc tcgccgctgc tgttagaact taacgaaaac gatccgggga tctttgtgac
     3841 tcagtcggtg cacaaacagc aggcgggatt ctcacagacg tcgcagatcc ataaaaaaga
     3901 taaccatatc cgcggacagg cgcgtttttg cccgcataag cggttgaata acgcctttat
     3961 gctccatgct tctaccagcc ctttctatcc gctgtttgct gcactggatg ttaacgccaa
     4021 aattcatgaa ggggagagtg ggcgtcggct gtgggctgag tgcgttgagt tgggtattga
     4081 gtcgcgcaag gctattcttg cgcgctgtaa gctgttccgc ccgtttatcc cgcccgttgt
     4141 tgatggcaaa ttgtggcagg attatccgac atcagtgtta gccagcgacc gtcgtttttt
     4201 cagttttgag ccgggggcga agtggcacgg ctttgaagga tatgccgcgg atcagtattt
     4261 tgttgatccg tgcaagctgt tactcactac accaggtatc gatgccgaaa ccggcgaata
     4321 tagcgacttt ggcgttccgg cgacgattct ggcgcactat ctgcgtgaga acggcattgt
     4381 gccggagaag tgcgatctca attccattct gttcttatta actccggcgg aaagccacga
     4441 gaagctggca caactggtgg cgatgctggc tcaatttgaa cagcatattg aggatgactc
     4501 gccgctggct gaggtgttgc cgagcgttta taacaaatat ccggtgcgct atcgcgacta
     4561 caccctgcgc cagttgtgtc aggagatgca cgatctgtat gtcagtttcg acgtcaaaga
     4621 cctacaaaaa gcgatgttcc gccagcagag tttcccgtca gtggtgatga acccccagga
     4681 tgcgcatagc gcttatattc gcggtgacgt ggagttggtg cggattcgtg atgccgaagg
     4741 gcgaattgcg gcagaagggg cgttgcctta tccacctggc gtgctttgcg tggtacccgg
     4801 ggaagtctgg ggtggggcgg ttcaacgtta tttccttgca ctggaagaag gggtgaattt
     4861 gttgccggga ttttcgccgg agctgcaagg tgtttatagc gaaaccgatg cggatggcat
     4921 gaaacggttg tacggttatg tgttgaagta agaataaaaa aaacgggtcg ccttctggcg
     4981 acccgttttt ctttgcgtaa ttagtggcta accgtctgtg tacctgtcgg gacacgaacg
     5041 tgtttatatt tgaacatcgc catgaacgcg aaggccagaa ccacggagta accagcgaaa
     5101 atcaaccata cggtctgcca gtcggtaata ccgttttggg tgtacatctc aacaacttta
     5161 ccgctcacga tgccgccgag gatacagccg aagccgttag tcatcatcag gaacatccct
     5221 tgtgcactgg cgcgaattgc cgggctaact tctttttcga caaacaccga accagagatg
     5281 ttgaagaagt cgaacgcaca gccgtaaaca atcatcgata gaaccagcag tacggtgccg
     5341 aacggagtcg ggtcgccgta agcaaacagc gcaaaacgca ggatccacgc cacaatactg
     5401 atcatcatta cgttcttaat accgtagcgg cttaagaaga acgggatggt cagaatgaac
     5461 agggtttcag agatctgcga aatcgacatg atgattgacg catgctgcac aataaagctg
     5521 ctggcaaaca tcggatcttt gtcgaagctg tgcaggaagg tattaccgaa catgttggta
     5581 atctgcagtt ccgcgcccag cagcattgag aagatgaaga agattgccat acgcttgttt
     5641 ttaaacagcg cgaatgcatc gaggcccagc agggttgtcc agctctgatt cgcttgctgt
     5701 ttagcaaccg gaatatgcgg cagagtcagg gtaaacagaa ccagaatggc ggaaagtgct
     5761 gcgccaatat acagctgcat gtggcttaat tcgaagccag acaggctcac cacccacatt
     5821 gccatgataa agccgatggt gccccagata cggattggcg ggaagtcagt aacgatatcc
     5881 atcccggcat tttgcaggcg atagtaagag atggtgttga ttaacccaag cgttggcata
     5941 taagcaaacg agttaatcaa tatcacaagg aacatcgcct ccggcgtcgt gacctgtgcc
     6001 gccatgaaca gcgtgatagc gccaatggtg tggcaaatgg catataccca tttcgcactt
     6061 aaccatttgt cggccacaat ccccagcagc gcaggcataa agaccgcggc gatacccagt
     6121 gagctataaa ctgcgccaat agaagcaccg tcaaacttca gggtaacaaa catataggaa
     6181 ccgagggtcg tcagccaact tccccacaga cagaactgca gaaaagagag gattttcagc
     6241 tgcagcttaa gattcatgtt aatttcctca catcgtgatg cggatgagtg tttgaaaaag
     6301 cacatttgac cgtgcgagat gatgggattc tatcaacttc gcggactggt tttttgttac
     6361 ctgtggcaaa taattgcaaa cattcctaat ttgcaaaacg aaattgtgat atggataaca
     6421 tttttgcccc tgagcatcgt caggggcggt tagtggaatt atcggcggcg gtaggatttt
     6481 tgcgcggtat tcactttata aagataacgg cgagattccg cagagggatg gcgggtcgtc
     6541 agcgtttgat aaacatcgcc cggcgtcatg gtgttaataa tattagccgc ctgaatctta
     6601 tcattcgaaa agactcgcag cacgctgcct gcgccgccgt tataggcggt gatgacggca
     6661 taacgccggg atgtagggtt atcaattccg ccgagataaa cattgttcag catcgccaga
     6721 tacgcggtgc cggtatcaat attgctggca ggatcaaaca agaaactgcg gctcggcgtg
     6781 ccggatttcc cctgcgagcg gaacacatct ttcccggcag tatgttgtac cacctgcatt
     6841 aaccccagcg catcggaacg gctgaccgca tacgggttaa aggaagattc agtctgcata
     6901 attgccagaa tcagcgactc atcaacgcca tatttccgtg acgcctggcg gaccatgccg
     6961 agatatttgt gcgcacgttt atcaaggtgg ttcggcacca tgttaatggt gacgctgtag
     7021 atgatacgca gcccgttgct gcggctcttc agacggtttt tcagcagata atccgcgaag
     7081 ttgcttgcgc gaccttccca gcgaatcggc tgcccggtgt tgtccaccac ctgaccgtaa
     7141 aggaaaggtt ctttcgaaat tgtaatatca tcaacgtcgg aatagagatc gaccgaactc
     7201 ggatcgtcac ccatcagcaa cgttttgata attgcccggc gcaaatgcgc ggcaggttct
     7261 gtcccggcga tggtttcgat agtaatcgta ccgtcatcga agttgatgtg gctgcgggtc
     7321 tgatattgat cggtgtattt cacgtagtcc ttaggaccgg cgatcaccac ctctttgaat
     7381 ccccagatgt tctcaatatt gtgggcaaat tgccccatca gaatatcaaa accgttggtg
     7441 tctttgaccc aggcttcgtt ataggtaccg ccttttttgg tcgtcgaaca ggagatgagc
     7501 aacggcgcaa tcaaagccag cgcgagatat tttttcatca ttccgggtgc gtgttgtgtt
     7561 tgtttttatg caagctgccg gaggcgtgct ccggcactgt ttttattttt tatcttccgg
     7621 cgtatagccc tcgatatgca cctctttacc ctcgaacagg aagttgacca tctcctgctc
     7681 cagcagcttg cggtgctcgg cattcatcat gttgagtttc ttttcattaa tcagcatggt
     7741 ttgtttgtgc tgccactgcg cccaggcttc tttggagatc tcgttataga tgcgttttcc
     7801 cagttcgccg gggtacagct gaaaatcctg accttctgct tcacgttgca ggaaagtaca
     7861 aaaaatcgtt ctgctcataa atcatcctct ttatcgactc acgcgctaaa ccggcgcgcc
     7921 agtgcgtaac tgctgtaaca aacgctccac gggagccgcc aggccaactg acggcggttg
     7981 cgctaagtta taccagagcg cattgccttc atccatgcag ccggtgaatg acgacacggg
     8041 aagccacata ggcacaatat ctaagtggaa atggctgaag gtatgccgaa acgcggtcag
     8101 ctgcgtcagg ttatcggcag caatctgccg ttgcgccagc cactgccgca aactttcttc
     8161 gtcggcaaac tgcgggaaac agtataaacc gccccacaat ccgctcggcg gacgctgcgc
     8221 cagcaatact tcatcttcgt gctgtaacag gagaaagtag ccggtgcgct ccggcagcgt
     8281 ctgtttcggt tttttgcccg gataaagcga ccagctattg ttggtggcgg caatacatcc
     8341 gttttgtagc ggacagagcg aacatttcgg cttcgaacgc gtgcaaatca tcgcgcccaa
     8401 atccatcatc gcctgattaa accgttccac gccgaccgcg ggcgtcacct gctcgcttaa
     8461 actccataat ttattctcga cctctttttt cccaggccag ccgcttacag catagcagcg
     8521 cgccagcacc cgtttgacgt taccgtcgag aatcggaaag tgcttaccca gagaaagcga
     8581 tagaatcgcg cctgcggtgg aacgcccgac gcccggcagt gctgcaactt cctcaaaggt
     8641 ttccgggaat ttaccgccgt gtaaggtcgc cacttgttgt gccgctttat gcagattgcg
     8701 cgcgcgggcg taatagccaa gccccgtcca caagtggaga acttcgtcga gcggcgcatt
     8761 ggcgagatcg gtcaccgtcg ggaagcgcgc cataaagcgt tcaaaatagg ggataacggt
     8821 cgcaacctga gtttgttgca acatcacttc tgagagccat actttgtagg gcgtcttgtc
     8881 aatttgccag ggcagagttt ttcgcccgta tttatcgtac cagtccagaa cctgggctga
     8941 aaattgcgac gcttgcatgg tcaccgaatt cactgttgtt gggggcaaga ttgcagcaca
     9001 gcgacggcag ggtgtaaacc ggaactttcc gcagccacgg ccttatcatg cttgcatccg
     9061 gcaactaact ttggataatg cccgttttca gaacactttc acaagcgact aaatctttat
     9121 gaaaaacgac gtcatttcac cggaatttga tgaaaacggc cgcccactgc gccgtatccg
     9181 tagttttgtg cgccgccagg ggcgactgac caaaggccag gaacatgcgc tggaaaacta
     9241 ctggccggtg atgggcgttg agttcagcga agatatgctg gatttccccg cgctttttgg
     9301 ccgtgaagcg ccggtgacgc ttgagattgg ttttggcatg ggggcgtcgc tggtggcaat
     9361 ggctaaagat cgccccgagc aggacttcct cggcattgaa gtgcattcgc cgggcgttgg
     9421 tgcgtgcctg gcttctgcgc atgaagaggg cttaagcaac ctgcgcgtga tgtgtcacga
     9481 tgcggttgaa gtgctgcata aaatgattcc tgacaattca ttgcgcatgg tgcagctctt
     9541 tttccctgac ccgtggcaca aagcgcgcca taataaacgc cgtatcgttc aggtgccgtt
     9601 tgccgaactg gtaaaaagca aactgcagct ggggggcgta ttccatatgg cgaccgactg
     9661 ggaaccttat gcggaacata tgattgaagt gatgtcttct attgacggtt ataaaaacct
     9721 gtcagagagc aatgattacg taccgcgtcc ggcatcacgt ccggtgacga aatttgaaca
     9781 acgtggtcat cgtcttggtc acggagtatg ggacttaatg ttcgagaggg tgaaataatg
     9841 gcaaagaacc gtagccgtcg tctgcgtaaa aaaatgcaca tcgacgaatt ccaggaatta
     9901 ggattttcgg tggcatggcg attcccggaa ggtacatcgg aagaacagat tgataaaacc
     9961 gttgatgatt ttattaacga ggttatcgaa ccgaacaaac tggcctttga cggcagcggt
    10021 tatctggcct gggaaggtct gatctgcatg caggaaatcg gcaaatgcac cgaagaacat
    10081 caggcgattg tgcgtaagtg gctggaagag cgcaacctgg atgaggtacg caccagcgaa
    10141 cttttcgacg tttggtggga ctaagaaagc atacgggcga tgacaaatgc aaaactgcct
    10201 gatgcgctac gcttatcatg cctacaaaga tgcacgatcg agtaggcggg ataaggcgtt
    10261 tacgccgcat ccggcatgga aaacgcgtac tttgttatca atctggggcc agcaaatgct
    10321 ggcctgattt gttcttgagg gaagactatg atgcgcaaaa tgctgctggc ggcagcactt
    10381 tcagtgacgg caatgaccgc tcacgccgac taccagtgca gcgtcacgcc gcgtgacgat
    10441 gtgattgtca gcccgcaaac cgtgcaggtg aagggcgaaa acggcaatct ggtgatcacg
    10501 ccagacggca acgtgatgta taacggtaag caatattccc tgaatgccgc ccagcgcgag
    10561 caggcgaagg attatcaggc tgaactacgt agcacgctgc cgtggattga tgaaggcgcg
    10621 aaaagccgcg tcgagaaagc ccgtattgct ctggataaaa ttatcgttca ggagatgggc
    10681 gaaagcagca aaatgcgcag ccgtctgacc aaacttgatg cgcagctgaa agagcagatg
    10741 aaccgcatta tcgaaacgcg cagcgatggc ctgacgttcc actataaagc cattgatcag
    10801 gttcgcgctg aaggccagca attagtgaat caggcaatgg gcggaatttt acaggacagc
    10861 attaatgaaa tgggcgcgaa agcggtgctg aaaagcggcg gtaacccatt acagaatgtg
    10921 ctgggaagcc tgggggggct gcaatcctca atccaaaccg agtggaaaaa gcaggaaaaa
    10981 gatttccagc agtttggcaa agatgtttgt agccgcgttg tgactctgga agatagccgc
    11041 aaagccctgg tcgggaattt aaaataatcc tctattttaa gacggcataa tactttttta
    11101 tgccgtttaa ttcttcgttt tgttacctgc ctctaacttt gtagatctcc aaaatatatt
    11161 cacgttgtaa attgtttaac gtcaaatttc ccatacagag ctaagggata atgcgtagcg
    11221 ttcacgtaac tggaggaatg aaatggagtt tttcaaaaag acggcacttg ccgcactggt
    11281 tatgggtttt agtggtgcag cattggcatt acccaatatc accattttag caaccggcgg
    11341 gaccattgcc ggtggtggtg actccgcaac caaatctaac tacacagcgg gtaaagttgg
    11401 cgtagaaaat ctggttaatg cggtgccgca actgaaggac attgcgaacg ttaaaggcga
    11461 gcaggtagtg aatatcggct cccaggacat gaacgatgat gtctggctga cactggcgaa
    11521 aaaaattaac accgactgcg ataaaaccga cggcttcgtc attactcacg gtaccgacac
    11581 gatggaagaa accgcttact tcctcgacct gacggtgaaa tgcgacaaac cggtggtgat
    11641 ggtcggcgca atgcgtccgt ccacgtctat gagcgcagac ggtccattca acctgtataa
    11701 cgcggtagtg accgcagctg ataaagcctc cgctaatcgt ggcgtgctgg tggtgatgaa
    11761 cgacaccgta ctggacggtc gcgatgtcac caaaaccaac accaccgacg tagcgacctt
    11821 caagtctgtt aactacggtc cgctgggata cattcacaac ggtaagattg actaccaacg
    11881 taccccggca cgtaagcaca ccagcgacac gccgttcgat gtctctaagc tgaatgaact
    11941 gccgaaagtc ggcattgttt ataactacgc taacgcatcc gatcttccgg ctaaagccct
    12001 ggtagatgcg ggctatgatg gcatcgtgag cgctggtgtg ggtaacggca acctgtataa
    12061 atccgtgttt gacacgctgg cgaccgccgc gaaaaacggt actgcagtcg tgcgttcttc
    12121 ccgcgtaccg acgggcgcta ccactcagga tgccgaagtg gatgatgcga aatacggttt
    12181 tattgcctct ggtacgctga acccgcaaaa agcgcgcgtc ctgctgcaac tggctctgac
    12241 gcaaaccaaa gatccgcagc agatccagca gatcttcaat cagtactaat catttcgccc
    12301 cggtatcgcg ccggggcttt ttcacttcag acttacgtcc attgccaatt ttaattgccc
    12361 taatgataat caccagaata aattattccg cgcgagggtt ttcgggtgaa aaagcaatgg
    12421 attgttggca cggcgctgct tatgttgatg actggtaatg tctgggcaga tggcgaaccg
    12481 ccaactgaaa atatcttaaa agatcaattc aaaaagcagt atcacggcat tctcaagctt
    12541 gatgccatca ccttaaaaaa tcttgatgct aagggtaatc aggccacctg gtcagcggaa
    12601 ggcgatgtct cttccagtga cgatctctat acctgggtcg gtcagttggc agattacgaa
    12661 ctgctcgaac agacctggac gaaagataaa ccggtaaaat tctcggcgat gttaaccagt
    12721 aaaggaacgc ccgcgtctgg ctggacggtg aacttttact cttttcaggc ggcagctagc
    12781 gatcgtggtc gggtggtcga cgatatcaaa acgaataata aatatctgat cgtgaatagc
    12841 gaagatttca attatcgctt tagtcagctt gagactgcgt tgaataccca gaagaactcg
    12901 attcctgcgc tggaaaagga agtgaaagcg cttgataagc aaatggtggc ggcgcagaaa
    12961 gcagcggatg catattgggg aaaagatgca aatggtaagc aaatgacccg cgaagatgcc
    13021 tttaaaaaaa ttcaccaaca gcgtgatgag tttaataaac agaacgatag cgaggcgttc
    13081 gcggttaaat atgacaaaga ggtttatcaa ccggcgattg cggcatgtca taaacagagt
    13141 gaagagtgtt atgaagtgcc gattcagcag aaacgagatt tcgatatcaa cgaacaacgg
    13201 cgacagactt ttctgcaatc acaaaaactt agccgtaaat tgcaggatga ctggataacg
    13261 ctcgaaaaag ggcaatatcc gttaacgatg aaagtttcag aaataaacag taaaaaagtc
    13321 gcgattttga tgaaaattga cgatatcaac caggctaatg aacgctggaa aaaagatacc
    13381 gaacaactgc ggcgcaatgg ggtgattaag taagttttga tatagcgcac gatttgtagc
    13441 aggatgcggc gttgaccccg catccggcaa tcaacgtctg atgcgacgct agcgcgtctt
    13501 atcagaccta caacagctgc cacatgtcgg ctgaggtgct cacgccgcat ccggcaatca
    13561 acgtctgatg cgacgcttgc gcgtcttatc agacctacaa ccgttgccac atgtaggctg
    13621 gataaggcgt tcacgccgca tccggcaata caagtttact cagccagaaa aagctccagc
    13681 agcgaattta aaaacagctt cccatgttcc gtaatctgcc agtaatccgc acattcggtg
    13741 agataaccct gggcaatcgc ctcgtctaac tgtgggcgaa tcacatcttc gcaaagcccg
    13801 gtatacgcac taaactccac gcgcggcgca ggttccagca accggaagcg attcataaag
    13861 aactcaaacg gcttatctgc ggcttcgaca tcacgctggc tttccagata ccttccttgc
    13921 ataaaaccac gcggatgacg cgttttggtg gtacgcagaa tgcgcccatc cgggaaggtc
    13981 actttgccgt gcgcgccgca gccaataccg atgtagtcac caaagcgcca gtagttgaga
    14041 ttgtgctggc actgataacc gggtttggcg taagcggaag tttcatattg ctgataaccc
    14101 gctgcggtta ataactgatg cccctgttcg aaaatatccc acagcgcgtc gtcgtccggc
    14161 agtaccggcg ggcgtgaacc aaacagcgta ttaggttcga tggtcagttg ataccaggaa
    14221 agatgcggcg gattcagttc aatagcctgg cgcagatcgc caagcgcctc ttccagcgat
    14281 tgatccggta gcccatgcat caaatcaagg ttaaagctac gtaaccctaa accgctcgcc
    14341 agctttgccg ctcgtttcgc ttcttgcggg ccatgaatac gtcccagccg tttcagcttt
    14401 tcttcgctaa aactctgtac cccaatagag atacgattta caccggcacg ctgataatca
    14461 acaaagcgat cggcttctac cgtaccgggg ttcgcttcca tggtaatttc tgcatccgct
    14521 gccagcggca aacgcgcacg cacgccgtcc agcagcgttt gcatcgccga gccggaaagc
    14581 aggctcggcg taccaccgcc aataaagatt gtctttactt cacggccctg agcgtaagcc
    14641 acatcgttgt ccagatcgtt aagcagatgc tgaacgtaat cgtcgtgcgg cacttctcct
    14701 ttcaacgcgt gagagttgaa atcgcagtac gggcatttct gcacgcacca cgggatgtga
    14761 atgtagagac tcagcggcgg taatttaacc attacgtaaa gcgtccagca gcagtttcaa
    14821 tgcctgacca cggtgggaaa tggcgctctt ttcttcgcgg gtcagttcgg cagcggtttt
    14881 cccttcggaa ggtacgaaga agattggatc ataaccaaag ccaccagtgc ccgccggttc
    14941 gcgagtaatc acacccggcc agctgccatg gcacaccagc ggggtcggat cttctgcgtg
    15001 acgcagatac accagcacgc agtggaaacg cgcctgacgt tggtcgtccg gtacgtcttt
    15061 cagtgtttcc agcagttttt gcagattctt ttgatcggtc gcgtcttcac cggaataacg
    15121 tgcggagtaa atacccggcg cgccgccaag cacatctacc gccaggccag agtcgtcggc
    15181 aattgccggt aaaccggtca cttttgccgc gtggcgcgct ttcagaatcg cgttttcgat
    15241 aaaggtcagg ccggtttctt cagcggaatc aacgccgagt tctgtttggg ccacgatatc
    15301 aagaccgaag tcgctaagca gcgacgccag ctcacgcact ttaccggcat tgccggttgc
    15361 gaggacaact ttttgcatag gatacctaat taattaacgc cgcgacttct ggcgggattt
    15421 gttgcggatt aatgatttta atttgtttgt ggcggccaag ttcgcctttt tcaatcacca
    15481 cctggctttt ggcaacccgg aattgcttac cgagaaactt caccagatgg ctgttggcct
    15541 gaccgtcaac cggcggcgcg gtaatggcga cttttacctc gtcgccatgt aaaccgacaa
    15601 tagaatcacg gctggctttc ggctgaatat agagccgtaa aaccagaccg tcatcgttaa
    15661 ctgttacggc actcataacg ccatccacag ccccggcagc agcatatttc cggtcgcctg
    15721 taatacttct gcgacaccca tattgatgac atacagcagc agaacgagga tcatcggcga
    15781 gaaatcaatc ccccccattg ccggtagcaa gcggcgaatc gggcgcagca gcggatcggc
    15841 cagctgaatc agcacgtatt caatcgggct acggccctgg cttacccagc tcataatcgc
    15901 catcaccagc aggacccaga aaatcagcag gccgatggtt ttcagcagaa tcagtaaacc
    15961 ggcaatccag atgattggca ggaaggtcac cactttaaac agcacgatgg ctttgataaa
    16021 actgagaata taggcaacca gcagcgaggc gctatcaatg ggtcccattg ccggaataac
    16081 gcggcgcagt ggcccgataa ttggctgcgt taccttcact acaaactgtg agaaggggtt
    16141 gtaaaaatca caatgagccc actgcatcca gatgcgtaat aacagcacca tggtatacag
    16201 ctcaatgacc gttgaaagca ggaaagtcaa cgtattcatg gcgttccttt aattccttat
    16261 tttttagagt aatcacgcgc accaaaaatt gcagtgccga tacgaaccat cgtgctacct
    16321 gccgcaatgg cggcttccat atcgtccgac attcccagag agagcgtgtc gatatgcggg
    16381 tagcgcgttt tcagtccggc aaatgctaca gccatttggc gtgcaacttc aaactgcctt
    16441 acatattctg actcaggcgc agggattgcc atcagcccgc gcagacgtaa acgcggtagt
    16501 tcagcgaccg cagctgccag ctcgtccagt tctgccagtt gaatcccgga cttactgttt
    16561 tcatcactaa tattaatttg aatcagtacg ttaagagggg gaagttctgc cgggcgctga
    16621 tcgttgaggc gggtagcaat gcgcaaacgg tcgatggtat gacaccagtc gaaatgctct
    16681 gccaccaggc ggcttttatt agactgcaac gggccaataa aatgccattc taatcctgtt
    16741 acgcccagtt cctgaaagtg gcgaatttta tctacccctt cctgaacgta gttttcacca
    16801 aattgacgct gcccggcatc aatggcttct gcgatggcgc tcgcaggttt tgttttactg
    16861 actgcaagca gcgtaatttc ttctggagaa cggccgcaac gcgttgcagc cgctgagatt
    16921 ttgtcccgga cctgtgccag gttatgcgca atatcgttca ttttccgagg atcttagtat
    16981 gaatatggaa gaaattgtgg cccttagtgt aaagcataac gtctcggatc tacacctgtg
    17041 cagcgcctgg cccgcacgat ggcgcattcg cgggctaatg gaagctgcgc cgtttgatgc
    17101 gccggacgtc gaagagctac tgcgggagtg gctggatgac gatcagcggg caatattgct
    17161 ggagaatggt cagctggatt ttgccgtgtc gctgacggaa aaccagcgat tgcgtggcag
    17221 cgcattcgcg caacggcatg gcatttcgct ggcgttacgg ctgttacctt cgcactgccc
    17281 gcagctcgaa cagcttggca caccaccgat attgccggag ttactcaaga gcgagaatgg
    17341 cttgattctg gtgacggggg cgacggggag tggcaaatct accacgctgg cggcgatggt
    17401 tggttatctc aatcaacatg ctgatgcgca tattctgacg ctggaagatc ctgtggaata
    17461 tctctatgcc agccagcgat gtttgatcca gcagcgggaa atcggtttgc attgtatgac
    17521 gttcgcatcg ggattgcggg ccgcattgcg ggaagatcct gatgtgattt tgctcggaga
    17581 gctgcgtgac agcgagacaa tccgtctggc gctgacggcg gcagaaaccg ggcatctggt
    17641 gctggcaacc ttacatacac gtggtgccgc gcaggcagtt gagcgacttg tggattcatt
    17701 tccggcgcag gaaaaagacc ccgtgcgtaa tcaactggca ggtagtttac gggcagtgct
    17761 gtcacaaaag ctggaagtgg ataaacagga aggacgcgtg gcgctatttg aattgctgat
    17821 taacacaccc gcggtgggga atttgattcg cgaagggaaa acccaccagt taccgcatgt
    17881 tattcaaact gggcaacagg tggggatgat aacgtttcag cagagttatc agcagcgggt
    17941 gggggaaggg cgtttgtgac gttgccatgc ccgatgcgca agcttatcgg gcctacaaag
    18001 gcaataaacg tctgatgcga cgcttgcgcg tcttatcaga cctacaaccg ctgccacatg
    18061 taggccggat aaggcgttca cgccgcatcc ggcaataagg cgtaaggcgc gatgatatta
    18121 tttacactcc agcaggcgat taaatctcct taatatccct gctcgaaata gctttcgaga
    18181 ataataaccg cagaggcaga gtcgattttg cctttgttga gcgcccgata gccgccctgt
    18241 tcaaacagac cggaacgtgc ctccacggtg ctaagacgct cgtcatggag ctttacttca
    18301 acaccgaaac gaccatgaat acggttagca aatttacgcg ctctggcggt taacggctgt
    18361 tcggtgccgt ccatattcag cggtaaaccg acgatgattt cgtctggctg ccactctttc
    18421 agtaaacgtt cgatgaggtt ccagtccggc gtaccgtcct gtgctttaat tgcaggcaaa
    18481 gggcgagcgg tgccggtaat gcgttggccg accgctacgc caatgctttt ggtgccgaag
    18541 tcgaaggcga gtaaggttcc actcatcagg cgtgtcctgc cacaccaggc atggtgagaa
    18601 tatccacacc aatcagtttt gccgcctcgc gccagcggtc ggcaattggc gttttgaaca
    18661 gaatattcag atctgccggg gcggttagcc acgcgttatc gagaatttct tgttccagtt
    18721 gacctttttc ccaggaggca tagcccagag ccaccaatac gtcagacggt tgtttatcgg
    18781 tgccgagcgt ttccagcaca tcgcgggaag tggtcatcac tgtgttgtct gaaatgcgaa
    18841 tgctggaagc aaaattggag ggcggagtat gcaaaataaa cccgcgatct tcagccagtg
    18901 ggccgccgag cataaccggt ttatccagac ggattgattc atcacgcggc tccggcgtga
    18961 tcttcagctt ttccagaatc ccttcaattt tgagattttc cagcggcttg ttgacgatga
    19021 tccccattgc accattggta ttatgttcgc aaatgtagac cacggaacga cggaaaatcg
    19081 gatcctggag agcaggcatg gcaataagaa agtgatgctg taaattcatt gtcagaggtt
    19141 ctgtttcctg gttcaaaaaa gcaacagcgc ccagtatgcg tggaaagaag ggcgctgtca
    19201 ctcagagtct caacgagatc cttctcgcta aggtgggtta ctactgctgt aaacgtgctt
    19261 cgatggcatc cattaacatt ccggtgatcg acaccggaaa ctctgcttca atctcacgaa
    19321 tacaggttgg gctggtgacg ttaatttcag tcagacggtc gccgatgata tccagaccaa
    19381 caaaaatcag ccctttttct ttcagcgtcg gcccgatctg acgggcgatt ttccagtcac
    19441 tttccgtcag cggacgaggt tcaccgcgac caccggcagc cagattgcca cgggtttcgc
    19501 ccccctgcgg aatacgcgcc aggcagtacg gtactggttc gccgtccacc accagcacgc
    19561 gtttgtcgcc atctttaatg gctggcagat aattttgtgc catgcagtag cgagtgccgt
    19621 gttcggtcaa ggtttcggca atgacgccga ggtttggatc gccttctttc acgcggaaaa
    19681 tcgacgcgcc gcccataccg tccagcggct taagaatgat gtcgctgtgt ttctcccaga
    19741 acgcttttag ctgcgcttta ttgcgcgtaa ccagcgtttc tggcgttaag tcagagaacc
    19801 aggcggtaaa cagtttctcg ttacagtcgc gcaggctctg cggcttgtta acgatcagcg
    19861 tccctttctc ttcggcacgt tccagaatat aggtcgcgta gataaactcg gtatcaaacg
    19921 gcgggtcttt acgcatcagg atcacatcga gatcggccag cggcagatcc tgttcaccga
    19981 cgaatgaaaa ccactcttcg tagttctgtt tcacgttcag cgtgcgggta tgggcgcggg
    20041 cttcgccatt gatcagatac agatcgccca tctccatata gtgaagttcg taaccacgac
    20101 gctgtgcttc cagcaacata gcgaaactgg aatctttctt gatgttgatg tttgcgatgg
    20161 ggtccatcac gatgccgagc ttgatcatta ttcttctccg ttagcccaaa tcgccaaatc
    20221 gtacttgtag cgcggtaatg gcggtgagcg cagttgtctc tgtacgcaaa acgcgaggtc
    20281 ccaacaggat atcagtaaat tgatagcggg cagtcatggc aatttcatct gccgataaac
    20341 cgccttccgg gccaatcagc aggcggacgc gttcaaccgg taaaggcaac gtattgatgc
    20401 tgttactggc gcgcgggtga agattcagtt tcagtccttc atcctgctct gcacaccagg
    20461 cttccagatc catcgctgga cggatttccg gcacccggtt acgaccacac tgctcacagg
    20521 cagcaattgc aatcttctgc cactgctgga gcttcttgtt cagacgttca ctatccagtt
    20581 taacgccgca gcgctcagaa aaaagtggcg taatgaggct tacaccgagt tcgatcgatt
    20641 tctggatcgt aaattccatt ttttcaccac gcgacatcac ctgaccgagg tgaatatgca
    20701 gcggagattc gcgatcgtcg atctggcctt ccagcacctt cacttccacg ctttttttgc
    20761 tggcgctggt aatttcggcg tcaaagacct ggttgctgcc gtcaaacaat tgcaacgcct
    20821 gtcccggccc catacgcagt acgcgcccaa tatggttggc ggcatcttcg caaagcgcga
    20881 tgtgagaatg gctggtcagt ggttcaggat gataaatgcg ggggatacgc atagttaaaa
    20941 atccgcgtcg tctccccacg cggttgtacg cgtggggtag gggttaacaa aaagaatccc
    21001 gctagtgtag gttagctctt tcgcgcctgg caagcgcgtt gcacatacgg gttatgattg
    21061 ccctgtacct tcgcgatgcg ttcatcgcgc tcgcactccc agtcggtaac cggatacatc
    21121 ttgtcccatg cgttgaacag ctgcgtttgc tggcgagaga gtgtcaggtt gtaatggtca
    21181 cgcatataga agtaggtgcg cgcaatggca ccgcgtgcac gcgctggtgg ttcggcagct
    21241 ttttctttga aatcgacctt catggcgcat tgaccgtact ggccttcacc gccattccac
    21301 tggctgtaca taaagttgcc gcgatcgcca ttcacctcgc cgactgacgg ttgcaggtta
    21361 tgcatatcgc tttccatctt gcgatagacc ggatctttgg cgcaattttt acgtccaccg
    21421 tcctgccagc actggcgctg gtgaccgaat tgccaggcgg gaacgacgtg ttcccactct
    21481 acgcggctgg cgcggttttc atttttgcgc acctgatagc cgcacgattg cagatcgaca
    21541 acgccttttt tgccctgcca gttaatttta catccgcaat aaaacgtacc gggcgcgtca
    21601 gcgtggactt ttaccgccgc ggctttcgcc tgagaaaaac tattgatacc ttcggccaac
    21661 gccgggccgg aaaatgctgc gctcagtacc accgcagcaa tagacaaata acggtacatc
    21721 acttactccg tgttaaaacg agccagcaac gtagcgaaag ttgttttggt atgcaatcag
    21781 ccagatcagg aaagttcctg atagttcaga tggttatttt gcgaccagtt gttcgccgca
    21841 gtgaacacag cgatagacgg cttcgccacg aactacgcga ttatgacggc gtacggtaag
    21901 ctgatgttcc tgacacttac agctgtaggg gaaggtgttg cggcgcacgg attgcagttc
    21961 gaactgatgc gtacggcggg ctggaacgcc cagcacactt tccatcatcc acttccactc
    22021 tttgccatgt ggtgctacgc ggccaaaatg tttccatacc agcaaatgtg ccagttcgtg
    22081 cggtaccact tcttcaataa aggcttcact gttttccaac agcaaaacgg gattaaggcg
    22141 aatttcatag ctttccagcc aggctgttcc ggcagaggtt ccgcgctggg tgtaagagag
    22201 ttttggctcc gggtagttac gccccaattt caggttggcc tgggcgagtt tttcccgcag
    22261 gcgacgcata acggcctgct ggatggcgat agggagacgg gatgttttca tagcggcaga
    22321 ggatagagcg aagaaaagat gactgcaaga ggtggcttcc tccacgacgg gaggaagcct
    22381 ggggagatta atcgtgagcg ccgatttcgc gcagtttacg acctttcatc agattacgtt
    22441 caatatgttc cagcgaaacg tgtttggttt ctggcaccag ccacagtgtc agcaggataa
    22501 acaggacgtt cagacccgcg taaacccaga aggtattagc gttgcccagc gtgttgagca
    22561 tggtcaggaa cgttgcgcca acgatcatgt tggcaatcca gttggtggcg gtggagcagg
    22621 tgatgccaaa atcgcggcct ttcagcggct gaatttcgga gcacagtacc caaatcagcg
    22681 gaccggcact catggcaaaa ccgacaataa acatcagcag catggcgatg gcgaaatact
    22741 gcgccgacgg agagtggata ccgatatgca tcattgtacc gagtacgccc ataccagcag
    22801 ccatcaccag gaagcccagc gttagcgttg gtttacgtcc ccagcggtca acaaggccga
    22861 ttgcgataaa ggtggcaagt acgttggtca ggccgacaat cactgtcccc cacatttgct
    22921 cggtggtgtt ggtataaccc gccagttcga agattttcgg cgcgtaatac atgatgacgt
    22981 tcatcccggt gaattgctgc attacctgta acagtacgcc aaggaacacc gcgcggcgga
    23041 agttgctatt ctctttaaac agcgcccagc cactctgttt aacctgcaaa ctttcacgga
    23101 tttcatccag ttcgcgtttc gcttccgcgc tggtgtcacg caggcgtagc agcacgcgtt
    23161 cggcatcaac aaaacggcgt ttggcggcaa accaacgtgg gctgtctggc aggaagaaga
    23221 caccaatcag cagcaaaatt gccgggatga taatcacacc cagcatccag cgccatgcac
    23281 cggtgtagct gaaggcggta tcagaaagat aagcaccgag gatcccgatt gtgatcatta
    23341 actgatacat cgagatcata ctgccgcgaa ttttttccgg cgcaatttca gagaggtaca
    23401 gtggtgcggt ataagaggcc acacctaccg ccagccccag cagaacgcgg gaaagaatca
    23461 gtacttcaac gtttggcgca gccgcagaga acagcgaacc ggcaacaaac aaaattgcgc
    23521 cgatcatcag gctctttttg cgcccgagtt taaaggagag ccagccgctg cccaccgcac
    23581 cgactgccgc accgaacatc atggagctta cgacccattc ttgcgtgtgc gaagtaatct
    23641 ggaattcatc tgcaataaac ggcagtgcgc cagcaattac accgatatcc aggccaaaga
    23701 gtaatcccgc cagagcggca aggaagcaga cgaaaaacgt cattgccttg tttgactgcc
    23761 cctgtttttt agcgtcaggc atgatgccct ccaatatggt tattttttat tgtgaattaa
    23821 gataggtgag tacgacgtaa aaagatgtga agcaaatcac atcag
//
LOCUS       DAZFAB010000071        23837 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZFAB010000071 DAZFAB010000000
VERSION     DAZFAB010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23837
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.071"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(15..3896)
                     /gene="entF"
                     /locus_tag="ACHJWA_003131"
     CDS             complement(15..3896)
                     /gene="entF"
                     /locus_tag="ACHJWA_003131"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFO4309679.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSELPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEQTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLAGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAAFWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQHYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYQFSYREMREQVVALANLLREHGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     ADVPDLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFVPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMAPVVLLQLPQLPLSANGKLDRKALPLPELKAQTPGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPLWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFEKIGPIIRATLNR"
     gene            complement(3893..4111)
                     /locus_tag="ACHJWA_003132"
     CDS             complement(3893..4111)
                     /locus_tag="ACHJWA_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbtH family protein"
                     /protein_id="HFO4309680.1"
                     /translation="MAFSNPFDDPQGVFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(4114..5316)
                     /gene="fes"
                     /locus_tag="ACHJWA_003133"
     CDS             complement(4114..5316)
                     /gene="fes"
                     /locus_tag="ACHJWA_003133"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFO4309681.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLISL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5559..7799
                     /gene="fepA"
                     /locus_tag="ACHJWA_003134"
     CDS             5559..7799
                     /gene="fepA"
                     /locus_tag="ACHJWA_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFO4309682.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWGQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDNDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGITWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            7974..8594
                     /gene="entD"
                     /locus_tag="ACHJWA_003135"
     CDS             7974..8594
                     /gene="entD"
                     /locus_tag="ACHJWA_003135"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFO4309683.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(8716..8868)
                     /gene="hokE"
                     /locus_tag="ACHJWA_003136"
     CDS             complement(8716..8868)
                     /gene="hokE"
                     /locus_tag="ACHJWA_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFO4309684.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(9219..9371)
                     /gene="hokE"
                     /locus_tag="ACHJWA_003137"
     CDS             complement(9219..9371)
                     /gene="hokE"
                     /locus_tag="ACHJWA_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFO4309685.1"
                     /translation="MLTKYALVAVIVLCLTVPGFTLLVGDSLCEFTVKERNIEFRAVL
                     AYEPKK"
     gene            9340..9607
                     /locus_tag="ACHJWA_003138"
                     /pseudo
     CDS             9340..9607
                     /locus_tag="ACHJWA_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9810..10928
                     /locus_tag="ACHJWA_003139"
     CDS             9810..10928
                     /locus_tag="ACHJWA_003139"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFO4309686.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHKEKD
                     YLLYKFNRFQACRYGLEGVITDPYTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            10994..11242
                     /locus_tag="ACHJWA_003140"
     CDS             10994..11242
                     /locus_tag="ACHJWA_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFO4309687.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTTFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            11307..11675
                     /locus_tag="ACHJWA_003141"
     CDS             11307..11675
                     /locus_tag="ACHJWA_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO4309688.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            11769..12422
                     /gene="nfsB"
                     /locus_tag="ACHJWA_003142"
     CDS             11769..12422
                     /gene="nfsB"
                     /locus_tag="ACHJWA_003142"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFO4309689.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            12530..13777
                     /gene="ybdG"
                     /locus_tag="ACHJWA_003143"
     CDS             12530..13777
                     /gene="ybdG"
                     /locus_tag="ACHJWA_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFO4309690.1"
                     /translation="MQDLISHVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(13845..15221)
                     /gene="pheP"
                     /locus_tag="ACHJWA_003144"
     CDS             complement(13845..15221)
                     /gene="pheP"
                     /locus_tag="ACHJWA_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFO4309691.1"
                     /translation="MKNASNVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLAMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(15323..18460)
                     /gene="cusA"
                     /locus_tag="ACHJWA_003145"
     CDS             complement(15323..18460)
                     /gene="cusA"
                     /locus_tag="ACHJWA_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFO4309692.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKIQVGPEMRRGIA
                     ELNGEVVGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRAID
                     NLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMSLG
                     GIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITNASVEVGPALFI
                     SLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGKIP
                     PESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQINEG
                     DLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMVET
                     TIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIGIK
                     VSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTVAD
                     VQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITLAD
                     VADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSVAF
                     SGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWLLW
                     WMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEALH
                     HGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFIIP
                     AAYKLMWLHRHRVRK"
     gene            complement(18472..19695)
                     /gene="cusB"
                     /locus_tag="ACHJWA_003146"
     CDS             complement(18472..19695)
                     /gene="cusB"
                     /locus_tag="ACHJWA_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFO4309693.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGAPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(19711..20043)
                     /gene="cusF"
                     /locus_tag="ACHJWA_003147"
     CDS             complement(19711..20043)
                     /gene="cusF"
                     /locus_tag="ACHJWA_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFO4309694.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGFDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSGIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(20067..21449)
                     /gene="cusC"
                     /locus_tag="ACHJWA_003148"
     CDS             complement(20067..21449)
                     /gene="cusC"
                     /locus_tag="ACHJWA_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFO4309695.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALENNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTVLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            21606..22289
                     /gene="cusR"
                     /locus_tag="ACHJWA_003149"
     CDS             21606..22289
                     /gene="cusR"
                     /locus_tag="ACHJWA_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFO4309696.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            22279..23727
                     /gene="cusS"
                     /locus_tag="ACHJWA_003150"
     CDS             22279..23727
                     /gene="cusS"
                     /locus_tag="ACHJWA_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFO4309697.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVFLLSGPTMMMPGHGHGHMEHSNWRMISLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELQFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     PGEAIVVRCQTVDHLVQVIVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSNARGTRFVIVLPERG"
BASE COUNT         5734 a         6506 c         6053 g         5544 t
ORIGIN      
        1 tagataatgt taatttatct gtttagcgtt gcgcgaataa tcggcccaat tttttcaaat
       61 gcccctggag agataatatc cacatgcgca caatcctgac gatagatatc cagctccgca
      121 atccacggcg accaggcgcg ttcggggctc ataccttcct gaagcgtacg ttcagcaaca
      181 aacagcgtcg cttttccgtc aaacggtacg ctatgagcag tcgtcagcag gcgcacagca
      241 tcagcgtagt tgccttcaat ggtggtaaac aactccgttg aagtacttcc ctgctgtgcc
      301 gccaggaagg cctcgcgctc gcggttaatc tccgccagca cttccgggtc cagaccatta
      361 gcttcttttt cctgccagtt ttgcgtttct ggcggccagg tatccagcaa gccaagaaat
      421 gccacctgtt cgccacgggc acgtagtcgc gccgcaatgc cctgcgccag tgtaccgcca
      481 agggaatacc ccagcaggta ataagggccg tgcggttgtt gttcaagtaa cgttgccaga
      541 tgcgcttcgc agacttcatc caggttcgtc gctgtctgca tggggccatg agggcgcggc
      601 gactgaatgc cgataatcga ccatagtgga tcgagataac gcgagagcac gctaaactgc
      661 caggcaaaac cggatgccgg atggaaacaa aacagcgtcg ggccattacc ttcacgcaac
      721 ggcagaatgg tttcgaatcc catgcgccgg gagctgtcct cttcaccatc aataatcgtt
      781 gccagtttgg cgacggttga cgcgaccatc acctgccccg gcgtcacctg acgggcaaac
      841 tgccgactta actgcgctgc cagtttcatt gccagtagcg aatgaccgcc aagcgcgaag
      901 aaatcagcat cggcatcctg cacgtcacaa cccagcaacg acgcgaatgc cgcagcgata
      961 atcgtttcac tgcccgcttt cggcgcacgc cccggcgttt gtgccttaag ttcaggcaac
     1021 ggtaaggctt tgcgatccag cttgccgttg gcgctaagag gtaactgtgg aagttgcagc
     1081 agaacgaccg gcgccatatg cggcggcaat gtttcgcgaa gctgtgcctg taatgcgctg
     1141 gtatccaacg gcagacctga ttgcgacacc agatagccca ccaactgacg cgcatcacca
     1201 ccggtggctg ccgcctggtt aatcacacag gcgtgggtaa cggcttgttc gacatccggc
     1261 agcgcctgca tcacgcgatc gatttcgccc agttcgatac gctgcccgcg aatttttagc
     1321 tgatcgtcac tgcgcccgag gtactccacc gcgccgttat ccagccagcg ggcaacgtct
     1381 ccggtacggt acatccgttc accagggaca aaaggatcgg caatgaagcg gctggcggtc
     1441 agatcgggtc gtccaagata cccctgcgcc agttgaatac cggtgagata gagatctccc
     1501 gccacacccg gcggcaccgg atgcatcatc gcatcgagaa tgcgcaagcc cgtattccac
     1561 accggataac caatcggcac actgctgccg cgcacctgtg ccagttcctc gccaaaagcc
     1621 ggataccaac tcacatctac cgccgcttcc gtcgggccat atagattatg caacggcgcg
     1681 cccgttaact gttgccattc gcggcataaa tcagccggta aggcctcacc actacagaaa
     1741 acctgtttca acgtcgcgca attctggcga gcggtttgcg gcgtcagcga tgcaacaaat
     1801 gccgccagca tcgacggcac aaagtgcgtg gtcgttacgc catattcggc aaagaattgc
     1861 tgcatagcga gcgggtcgcg gtgcgcttcc ggttcagcca tcaccagttt agccccggca
     1921 ataaacggcc agaaaaactc ccacaccgag acatcaaaac tgcacggcgt tttttgggca
     1981 acgacatctt cacctgtaag tggataatga ttttgcatcc acaacaggcg gttaacgata
     2041 gccgtctgcc cgaccattac ccctttcggc ctgccggtgg agccagaggt aaagatgata
     2101 taagccgtgt gatgcggttg tgaaagttgc agcggcgcac tgccctgcgg tgtaagcggg
     2161 gcgttatagc aaaggttggt taaatctgga acatcggcaa agcgcggcag ttgatcgtcg
     2221 gtggtgatta acagcgacgg acgcgcatct tccagcatca ttttcaggcg atcgtccgga
     2281 taaccggtat ccagcggtaa ccaggccgca cctgcttcaa caatcgcatg tagcgccagg
     2341 gtcaaaaaga ccgagcgcgg taatgccacc gccacgctgt cccctggttt aacgccgtgc
     2401 tcacgcagca gattcgccag cgccaccacc tgctcgcgca tttcccgata gctgaactgg
     2461 taacgcgcat ctgccagcgc cggagcatcc ggtgtttttg ccgcttgttc tgccaccagc
     2521 gcgctaagcg tggtttctgg aatctcaacc tgagtggcgt tgatctgcgc cagctgcgca
     2581 tactcacctg gcagcataat atcaacatcg ccgcacaaca gagccggatc cgcagcgaat
     2641 tgggcgatca gcattttcag gcgttcagca tgctggatta acgttggctc atcgtaatgc
     2701 tgtttattgg cgaggatctc aatactcaaa tcaccgtgtt catccgggaa cagggccagt
     2761 tcaaggtcat taaccggacc ggttgccagg gtatgggttt gcgcctgaac accaggaata
     2821 tccagttggt aatcaaatac cttgatattg agtaccggac caaacagcgg ttcatcacct
     2881 gccgctcgcc cgctgtcacg gacaatttgt tcggcatcgt aacgttgatg acgacgcatt
     2941 tttttcagtt gtgctgccag tcgggttgcc agttccggca gcgtttcttg tgccgcaatg
     3001 tgaataccca acggtaaaac gttgagcacg ggtccggtag ccgtcagcgc cgccgagccc
     3061 agtcgacgca taaagataaa tccggcggcg tagtccatgc gattgcacaa tcgccccagc
     3121 caaaaggctg ccagcgcaag ggctaaatcg gtacgctgca cacctgaaag ttgcgtagcc
     3181 agctggcgga attccccgtc ggtaaattcc agtttcaggc gtagaatatc tgccgaagcg
     3241 ctgcgccccg ctaaaggtgc cggagaaagt gacgcgggcg gcggcagttg acgacgctgt
     3301 tccgcccaga atgccgcatc acgctgccag gcttcgcttt cgcggtattg ctggtactct
     3361 tccactacat cagcgaaagg cgtaaacggc gaagcaggcg tttgttcgcc acgcagcaat
     3421 gcgcagtaaa tgttggcgat ctgccgagta atggccggga aactgaagcc atcgaccagc
     3481 aaatggtgat aacgctgata ccagtaccag cggttatctg ccacctgtat cagctggtga
     3541 aagaccagtg gtttaccgct atcgacgcgc aaatcttgtt gcaaatccgc ctgcattaat
     3601 gcctgcgcag taccgtgcgg atcaatattg gttcgcaggt cgataatttc tggcagttcg
     3661 aatatcagcg catcatcgac ccattgccag acttcgccgt tatcttccgt aaaacgcatc
     3721 cgcagcgtat ctgcttgcgc taatccggca accaccgcgc gggccagtaa tggcgcatca
     3781 acctctccgg ttaactcaac gtaatgcgcc acgctccagg cggagggtaa ttctgacagt
     3841 ttttctgcca tccagatgcc gggctgtgcg gcgaccaaag gtaaatgctg gctcattgtg
     3901 cctcctgcaa ctgggtgaaa ttcgccggtg tcagagtacg ccagtggact tccagccact
     3961 gctggcagga ctcttgtgac tgcggctgac acacaatgtc ccagcctgcc ggtaaggcgc
     4021 attgttgcgg ccacagactg aattgcccct gcgcattgcg caaaatgtaa aacactccct
     4081 gtggatcatc gaagggatta ctgaatgcca tattcaactc ctgtcatgga aaagtggttg
     4141 ccagaggtcg attaacccct gcatcaagcc accgcgccaa caaagcgcat catgtccgcc
     4201 gtcaacctga cgccagaaaa tggattcttt tatagggtgt aattgcgcat acagcgcctg
     4261 attggcccgc atgatcatcg gctcgcgaat acccgcttcc agcacaatgc gcagaccttc
     4321 ggcgctaact tcaccagctt ttagcttttc aagtaacacg ccctcttgct gcccgccccg
     4381 atgcggccac cagtacgatc ctgactggct taatacacag ccaaagcgtt caggccagtg
     4441 cagtccggca tacagcgcgg acagcccacc aaaactctgc ccggcgacca cggtgcgatc
     4501 ggcgcgatcg ctaaaagggg caatagcttt caccaggggt aataactctt gctgtactgc
     4561 cagccagaaa tccgcattac acggcagttc gtgggcgcgg tgcgtggtgt cgatagcgtc
     4621 gatcaacaca tacacggcgg gaggaagttg ctgacgatgg gtcagcgaaa tcagcacagg
     4681 ccagacgggc atactttgcg cccaaaattc gccatcgagc aaaactgcca gcgggcgttc
     4741 ttctgctgtt acatcgccgg tggtaaaaat ccatacacga cgtgaatttt tcaaccgttc
     4801 acttttccag ataatttctt tggctggtat ttctggcgct tgcggacaat cccatcccgg
     4861 ttgcagaggc gcttgcggca tttcgagtgc agaaacagcg tgccctcgcc cgcctttcca
     4921 gctttgtgga ttcagcggat cggctattgc ctggggtaat agttttcgcc agccttcacg
     4981 taattcgagg cgatcggggg atggtgcaga aaaaatgtca tcgcgttcgg tgggaataaa
     5041 gcagtagctg ccgcgccagt tggcattgag ttgtgtcgtc cactgccaga catcagtacc
     5101 tgcaattcgc tgcatcgact ggggctggct gttctgatga tgatcggtca caccagtgat
     5161 gtagatccat acgcgcttta tcgtcgagta ttcttcagaa ccttggggat cacgccacca
     5221 gaaagtgacc tcaaacattt cgtcattcag acgctgccat tccgggccat gtttcgactg
     5281 ccaccagctc tcacttccta cttttaacgc cgtcacacca taaccccatg tttactgtgc
     5341 aatttttcat tgattgcaga aatatattga taatattatt gataactatt tgcatttgca
     5401 atagcgtaat ggcgcgccgt gggaagcgcg gacattaatt aaccaactgc actgcgtgtc
     5461 tttcaggatc aaaggttttc gcggtagcgg gatgcgtcgt gttgatgacg accatgcccg
     5521 acagttgcaa ttcgtggcaa aaatgcagga ataaaacaat gaacaagaag attcattccc
     5581 tggccttgtt ggtcaatctg gggatttatg gggtagcgca ggcacaagag ccgaccgata
     5641 ctcctgtttc acatgacgat actattgtcg ttaccgccgc cgagcagaac ttacaggcgc
     5701 ctggcgtttc gaccatcacc gcagatgaaa tccgcaaaaa ccccgttgcc cgcgatgttt
     5761 cggagatcat ccgtaccatg ccaggcgtta acctgaccgg taactccacc agtggtcagc
     5821 gagggaataa ccgacagatt gatattcgcg gtatgggtcc ggaaaacacg ctgattttga
     5881 ttgacggcaa gccggtaagc agccgtaact cggtgcgtca gggctggcgt ggcgagcgcg
     5941 atacccgtgg tgatacctcc tgggtgccac ctgaaatgat tgaacgtatt gaagttctgc
     6001 gtggtccggc agctgcgcgt tatggcaacg gcgcggcggg cggcgtggtt aacatcatta
     6061 ccaaaaaagg cagcggcgag tggcacggct cctgggacgc atatttcaat gcgccagaac
     6121 ataaagagga aggtgccacc aaacgcacta actttagcct gaccggtccg ctgggcgacg
     6181 aattcagctt ccgcttgtat ggcaacctcg acaaaaccca ggctgacgcg tgggatatca
     6241 accagggtca tcagtccgcg cgtgccggaa cgtatgccac gacgttacca gccgggcgcg
     6301 aaggggtgat caataaagat attaatggcg tggtgcgctg ggacttcgca ccattgcaat
     6361 cgctggaact ggaagcgggt tacagccgcc agggtaacct gtatgcgggc gatacccaga
     6421 acaccaactc tgacgcttac actcgctcga aatatggcga tgaaaccaac cgcctgtatc
     6481 gccagaacta ctcgctgacc tggaacggtg gctgggataa cggcgtgacc accagcaact
     6541 gggggcagta cgaacacacc cgtaactcgc gtattccgga aggtctggcg ggcggtaccg
     6601 aagggaaatt taacgaaaaa gcgacacagg atttcgtcga taacgatctt gatgacgtga
     6661 tgctgcatag cgaagttaac ctgccgattg atttcctcgt taaccagacg ctgacgctgg
     6721 gtacggagtg gaatcagcag cggatgaagg acttaagttc caacacccag gcactgaccg
     6781 gaacgaatac cggtggcgct attgatggcg tgagtgccac cgaccgtagc ccgtattcaa
     6841 aagcagaaat tttctcgctg tttgccgaaa acaacatgga gctgactgac agcaccatcg
     6901 taacgccggg gctgcgtttc gatcatcaca gtattgtcgg caataactgg agcccggcgc
     6961 tgaacatatc gcaaggttta ggcgatgact tcacgctgaa aatgggcatt gcccgcgcct
     7021 ataaagcgcc gagcctgtac cagactaacc cgaactacat tctctacagt aaaggtcagg
     7081 gctgctatgc cagcgcgggc ggctgctatc tgcaaggtaa cgatgacctg aaagcagaaa
     7141 ccagcatcaa caaagagatt ggtctggagt tcaaacgcga cggctggctg gcgggcatca
     7201 cctggttccg taacgattat cgcaataaga ttgaagcagg ctatgttgct gtagggcaaa
     7261 acgcagtcgg caccgatctc tatcagtggg ataacgtgcc gaaagcggtg gttgaaggtc
     7321 tggaaggatc gttaaacgta ccggttagcg aaacggtgat gtggactaat aacatcactt
     7381 atatgctgaa gagtgaaaac aaaaccacgg gcgaccgttt gtcgatcatc ccggagtata
     7441 cgttgaactc aacgctgagc tggcaggcac gagaagattt gtcgatgcaa acgaccttca
     7501 cctggtacgg taagcagcag ccgaagaagt acaactataa aggtcagcca gcggttggac
     7561 cggaaaccaa agaaattagt ccttacagca ttgttgggct gagcgcgacg tgggatgtga
     7621 cgaagaatgt cagtctgacc ggcggcgtgg acaatctgtt cgacaaacgt ttgtggcgtg
     7681 cgggtaatgc ccagaccacg ggcgatttgg caggggccaa ctatatcgcc ggtgccgggg
     7741 cgtataccta taacgagccg ggacgtacgt ggtatatgag cgtaaacact cacttctgat
     7801 gctaacgtca gattgttgac aaagtgcacg tcgttcatgc cggatgcggc gtgaacgcct
     7861 tatccggcct acaaaaatct tgccaattca atatattgca ggaccatgta ggcctgataa
     7921 gcgtagcgca tcaggcagtt ttgcgtttgt catcagtctc gaatatggtc gatatgaaaa
     7981 ctacgcatac ctccctcccc tttgccggac atacgctgca ttttgttgag ttcgatccgg
     8041 cgaatttttg tgagcaggat ttactctggc tgccgcacta cgcacaactg caacacgctg
     8101 gacgtaaacg taaaacagag catttagccg gacggatcgc tgctgtttat gccttgcggg
     8161 aatatggcta taaatgtgtg cccgcaatcg gcgagctacg ccaaccggtg tggcctgcgg
     8221 aggtatacgg cagtattagc cactgcgggg ctacggcatt ggccgtggtt tcccgtcaac
     8281 cgattggcgt tgatatcgaa gaaatttttt ctgcgcaaac agcgaccgaa ttgacagaca
     8341 acattattac accagcagaa cacgagcgac tcgcagactg cggtttagcc ttttctctgg
     8401 cgctgacact ggcattttcc gccaaagaga gcgcatttaa ggcaagtgag atccaaactg
     8461 atgcaggttt tctggactat cagataatta gctggaataa acagcaggtc atcattcatc
     8521 gtgagaatga gatgtttgct gtgcactggc agataaaaga aaaaatagtc ataactctgt
     8581 gccaacacga ttaattgaca acatctggta cgattcgccc gcagccatca ctgactgcgg
     8641 gcgaaagtgt aaagcaggtg ccttaccact ctgacctgac aaccggatat gcggggattg
     8701 ctccccgcac aacggctact tcttcggttc gtaagcgaga acagccttaa actcaatatt
     8761 acgttccttt acggtgaact cacacagcga gtctccgacc agaagcgtaa atcccagcac
     8821 cgtcaaacac agcactatga ctgccacaag ggcatatttc gtcagcatgt ttatattgcc
     8881 tccatgcgaa gaagggacta ccatccgagt tgttggggct cggagtggca gccccaggat
     8941 tgatagaaat atctcctggg gcttcatctt tccagacctc agagttaacc tgaaaccgga
     9001 aagcttcagg cacccgccgt tatcttaccc ttccttagca aaatagctgt ttatttatac
     9061 agtgaatcct gtttcggact tctgtcggca aaactcatca ttgctcttaa aacgaaatcg
     9121 cccgcagcca tcactgaccg cgggcgaaag tgtaaagcag gtgccttacc atcctgacct
     9181 gacaaccgga tatgcgggga ttactccccg cacaacggct acttcttcgg ttcgtaagcg
     9241 agaacagccc tgaactcaat attacgttcc ttcaccgtaa actcacacag cgagtctccg
     9301 accagaagcg taaatcccgg taccgtcaaa cacagcacta tgactgccac aagggcatat
     9361 ttcgtcagca tgtttatatt gcctccatgc gaagaaggaa ctaccatcca aattgtctgg
     9421 tttggagtgg cagccccagg gttgatagaa atatctcctg gagcttcatc tttccagacc
     9481 tcagagtaaa cctgaaaccg gaaagcttca ggcacccgcc gttatcttac ccttcctcaa
     9541 caaaatagct gtttatttat acagtaaatc ctgtttcgga cttctcactc ctcatttaat
     9601 caactgaagc tgagcccgtt ttttctgcat gcctcaatcg tcaaaattct tcgccaccag
     9661 ccagccgtgc cataacttgt cacattgcaa aagtagccaa attgcttcag tcaccatcac
     9721 caaaaaaaga aacattccag aacttccgca tctcgcccag ccgctccgac ttctatactg
     9781 aatagaaaac tccaacataa gagaaaccta tgccattacc cgattttcat gtttctgaac
     9841 cttttaccct cggtattgaa ctggaaatgc aggtggttaa tccgccgggc tatgacttaa
     9901 gccaggactc ttcaatgctg attgacgcgg ttaaaaataa gatcacggcc ggagaggtaa
     9961 agcacgatat caccgaaagt atgctggagc tggcgacgga tgtttgccgt gatatcaacc
    10021 aggctgccgg gcaattttca gcgatgcaga aagtcgtatt gcaggcagcc gcagaccatc
    10081 atctggaaat ttgcggcggt ggcacgcacc cgtttcagaa atggcagcgt caggaggtat
    10141 gcgacaacga acgctatcaa cgaacgctgg aaaactttgg ctatctcatc cagcaggcga
    10201 ccgtttttgg tcagcatgtc catgttggct gtgccagtgg cgatgacgcc atttatttgc
    10261 tgcacggctt gtcacggttt gtgccgcact ttatcgccct ttccgccgcg tcgccatata
    10321 tgcagggaac ggatacgcgt tttgcctcct cacgaccgaa tattttttcc gcctttcctg
    10381 ataatggccc gatgccgtgg gtcagtaact ggcaacaatt tgaagccctg tttcgctgtc
    10441 tgagttacac cacgatgatc gacagcatta aagatctgca ctgggatatt cgccccagtc
    10501 ctcattttgg cacggtggag gttcgggtga tggatacccc gttaaccctt agccacgcgg
    10561 taaatatggc gggattaatt caggccaccg cccactggtt actgacagaa cgcccgttca
    10621 aacataagga gaaagattac ctgctgtata aattcaaccg tttccaggcc tgccgctatg
    10681 ggctggaagg cgtcattacc gatccgtaca ctggcgatcg tcgaccacta acggaagaca
    10741 ccttgcgatt gctggaaaaa atcgcccctt ctgcacataa aattggtgca tcgagcgcga
    10801 ttgaggccct gcatcgccag gtcgtcagcg gtctgaatga agcgcagctg atgcgcgatt
    10861 tcgtcgccga tggcggctcg ctgattgggc tggtgaaaaa gcattgtgag atctgggccg
    10921 gtgactaagc cgggaattgc cttacagaag aatatccctg acaatggtgt tttaacttac
    10981 atttaacaat gttatgaaac accctttaga aaccttgacc accgcagcag gcattttgct
    11041 gatggctttc ctctcttgcc tgctgctgcc cgcccccgca ctggggctta cgctggcaca
    11101 aaaactggtg accacgttcc atctgatgga tcttagtcag ctttacactt tattgttttg
    11161 tctgtggttt ttagtgctgg gcgctattga gtattttgtt ctgcgcttta tctggcgacg
    11221 ctggttctcg ctggcggatt aaacgtcgat tatcgccgta cttcgcggca tactttgctt
    11281 attctccttc gcctaaagga acgtttatgg ataagcaatc actgcacgaa acggcgaaac
    11341 gcctggccct tgagttaccc tttgtcgagc tttgctggcc ttttggcccg gagttcgatg
    11401 tttttaaaat tggcggcaag atttttatgc tgtcgtcgga gctacgcggc gtccccttta
    11461 tcaatctgaa gtccgatcca caaaaatccc tgttaaatca gcaaatatac ccaagcatta
    11521 agccagggta tcacatgaat aaaaagcact ggatttcggt gtatcccggc gaggaaatct
    11581 ccgaagcgtt acttcgcgat ctgatcaacg attcgtggaa tctggtggtt gatggtctgg
    11641 ctaaacgcga tcaaaaaaga gtgcgtccag gctaaagcgg aaatctatag cgcatttttc
    11701 tcgcttacca tttctcgttg aaccttgtaa tctgctggca cgcaaaatta ctttcacatg
    11761 gagtctttat ggatatcatt tctgtcgcct taaagcgtca ttccactaag gcatttgatg
    11821 ccagcaaaaa acttaccccg gaacaggccg agcagatcaa aactctcctg caatacagcc
    11881 catccagcac caactcccag ccgtggcatt ttattgttgc cagcacggaa gaaggtaaag
    11941 cgcgtgttgc caaatccgct gccggtaatt atgtgttcaa cgaacgtaaa atacttgatg
    12001 cctcgcacgt cgtggtgttc tgtgcaaaaa ccgcgatgga cgatgcctgg ctgaagctgg
    12061 ttgttgacca ggaagatgct gatggccgct ttgccacgcc ggaagcgaaa gccgcgaacg
    12121 ataaaggtcg caagttcttc gccgatatgc accgtaaaga tctgcatgat gatgcagagt
    12181 ggatggcaaa acaggtttat ctcaacgtcg gtaatttcct gctcggcgtg gcggctctgg
    12241 gtctggacgc ggtacccatc gaaggttttg acgccgccat cctcgatgca gaatttggtc
    12301 tgaaagagaa aggctacacc agtctggtgg tagttccggt gggtcatcac agcgttgaag
    12361 attttaacgc tacgctgccg aaatctcgtc tgccgcaaaa cattacctta accgaagtgt
    12421 aattctccct tgccgggcat ctgcccggct atttcccctc agattctcct gatttgcata
    12481 accctgtttc agccgtcatc ataggctgct gttgtataaa ggagacgtta tgcaggattt
    12541 aatatcccat gttgaagatt tagcgggtat tgagatcgat cacaccacct cgatggtgat
    12601 gattttcggt attatttttc tgaccgccgt cgtggtgcat attattttgc attgggtggt
    12661 actgcggacc ttcgaaaaac gtgccatcgc cagttcacgg ctttggttgc aaatcattac
    12721 ccagaataaa ctcttccacc gtttagcttt taccctgcag gggattatcg tcaatattca
    12781 ggcggtattc tggctgcaaa aaggcaccga agcggcagat attctgacta cctgcgcgca
    12841 gttgtggatc atgatgtatg cgctgctttc agtcttctcg ctgctggatg ttattttgaa
    12901 tctggcgcag aaattcccgg cagcatctca gttaccgctg aaagggatat ttcaggggat
    12961 taaactgatc ggcgcgattc tggtcggcat tttgatgatc tcgctgctga ttggtcagtc
    13021 gccagcgatt ctgatcagcg gtcttggtgc aatggctgcc gtgctgatgt tggtatttaa
    13081 agatccgatt cttggtctgg tggcaggtat tcagctttcc gcgaacgata tgctgaaact
    13141 gggcgactgg ctggagatgc cgaaatacgg cgcggatggc gcggtgatcg atattgggtt
    13201 aaccaccgtc aaggtgcgta actgggacaa taccattacc actattccca cctggtctct
    13261 ggtttctgac tcctttaaaa actggagcgg gatgtcagca tctggcgggc gacgtattaa
    13321 gcgcagtatc agtattgatg tcaccagtat tcgttttctt gatgaagacg aaatgcaacg
    13381 tctgaataaa gcgcatttgt taaagcctta tttaaccagc cgccatcagg aaattaatga
    13441 gtggaatcgc cagcaaggtt ctacggagtc ggtattaaac ctgcgccgaa tgaccaatat
    13501 tggaaccttt cgtgcctatc tgaacgaata tctgcgtaac catccgcgga ttcgtaaaga
    13561 tatgacctta atggtacgcc aactggctcc aggtgataac ggtttaccgc tcgagatcta
    13621 tgcgtttacc aacaccgtgg tgtggctgga atatgaaagc attcaggctg atatattcga
    13681 tcacatattt gcgattgtcg aagagtttgg tctgcgactt catcagtcgc caaccggcaa
    13741 tgatattcgc tctctggcgg gtgcatttaa gcagtaatta aaaaaaccgc tctcatcgaa
    13801 tggatgagag cggacatcgt tttgtcggat gtagcgtgaa tgccttattt ccgacgcagc
    13861 gttttaaatg ctacaaacag gaatacaatc cacaccggca gcaggatcgc tgacaagcgc
    13921 atatcatcca tcgtgcacat cagcagcaaa atcatggcga ggaaggcgat gcaaagatag
    13981 ttgccgaacg gatagagcag cgccttaaac tgtgtttcac gcccctgacg tcgcatcgct
    14041 gcacgaaaac gcagatgcgc cagacagatc ataatccagt tcaacagcag cgttgctacc
    14101 accagcgcca tcagcagacc aaacgctttt tgcggcagca gatagttgat taacaccacc
    14161 agcgaagtga tcgctccgga aagcatcagc gagttaatcg gcacgccgcg acggctaaca
    14221 cgggtaagga acttcggcgc attaccctgc acagaaaggc caaacagcat gcggctgtta
    14281 gagtaaaccc cgctgttata cactgacagc gatgctacca gaatgacgaa gttcagcgca
    14341 gaagctacca cgttgctgtc gagattatgg aaaatcatca caaacgggct actgttggat
    14401 ttcacttcca cccacggata gagcgccagt aaaaccacca gtgaaccgat gtaaaacagc
    14461 aggatgcgat acaccacctg atttaccgct tttggaatgc ttttttccgg atcgcgcgct
    14521 tcagcggcag taatcccaat cagctccaga ccgccgaagg agaacataat taccgccagc
    14581 gacaaaatca gcccattcca gccggtggcg aagaaaccac cgtagcgcca gaggttgtcg
    14641 atactggctt tctcgccgcc gtgaccagaa aacagcagcc acaggccaaa gccgatcata
    14701 ccgatgattg ccagcacttt aatcagcgca aaccagaact cggtttcgcc atataagcgc
    14761 acgttcacca ggttaacggc gttgatgata ataaagaagg cggcagccca aatccacgtt
    14821 ggaacatccg ggaaccagta ctgcatatag atgcccgcag cggtcagctc tgccattccc
    14881 accagcacga acatcaccca gtagttccag ccggagagga acccggcgaa cggtccccag
    14941 tatttatagg caaagtgggc aaatgaaccg gataccggct cttcgaccac catctcgccg
    15001 agctggcgca taatcaggaa agcgatgatc ccggcgacgc cgtagcccag caatacagcc
    15061 ggacccgcca tctgaatcgc cgggccaatg ccaagaaaca gaccagtacc aattgcgcca
    15121 cccagcgcaa tcagttgaat atgacggtta tgtaatccgc gatgaagcgt cggctcttga
    15181 ttcgacgcag tatcttccga tacgtttgac gcgtttttca cgcctttccc ctgtgtgtct
    15241 tttttgttga ggggcacctt ttaacattta gtgcccatcg tcgcaagaca caatccacac
    15301 ggttaaaccg ggtatcctgc ttttatttcc gtacccgatg tcggtgcagc cacatcagct
    15361 tatacgctgc cgggataata aacagcgaca gcaaaggcgc ggtgatcatg ccgccaatca
    15421 tcggcgcggc aatccggctc atcacctctg atcctgcccc cgttccccac agaatcggca
    15481 gcagaccagc gataatcacc gccaccgtca tcgctttcgg gcgcacgcgc aggaccgcgc
    15541 cgtgatgtaa cgcctcatcc agcttctgct cgctgaatgt ttgcggatta ttcaacgacg
    15601 gctcggcctc tatggcgtga cgtaaataca tcagcatcac cacgccaaat tcggcggcga
    15661 ccccggcaag ggcaataaaa ccggtgcccg tcgccacaga aagatgaaag cccatccacc
    15721 acagcaacca gatgccgccc accagcgcaa acggtacgct gctgataatc agcaacgctt
    15781 cgcccacccg acggaacgcc agatacaaca gcacgaagat gatcatcaac gtcatcggca
    15841 ccatgagttt cagcttatgg ttggcgcgct ccagtagctc gaactgcccg gagaatgcca
    15901 cgctggtgcc cggttttaac tgcacttttt cagctatcgc tttttgcaaa tcgtgaacca
    15961 ccgacaccat gtcacgatcg cgggcatcga tataaatcca gctcgtcggg cgcgcattct
    16021 cggttttcag catcgacggt ccggtagaga ctttaacgtc ggccacgtct gccagggtga
    16081 tttgctgctt catcggcgtc aggatcggca actggcgcag tgcctgcgga ctatcgcgcc
    16141 agctttgcgg ataacgcaga ttaattggat aacgggcaat cccttccacc gtttcgccaa
    16201 ccatcgcccc gcccaccgca gaagtcacaa acaactgcac atccgccacc gtcataccgt
    16261 aacgtgcggc tttttcacgg ttaatctcaa cgttgatata gcgcccacct tccagccgct
    16321 cggcaagcgc agaagccacg cctggcaccg ttcgtgccac ctcttcaatt tgctccgcca
    16381 tcgcgtcgat atccgccagc acagtgccgg aaacttttat gccgataggg cttttaatgc
    16441 cggttgagag catatcgata cggttacgaa ttggcggcac ccacagattc gccagccccg
    16501 gcagacgcac ggtgttatcc agttcctcaa tgattttgtc cattgtcatg cctggccgcc
    16561 actgctcctg cggcttaagc tggatggtcg tttctaccat ctccagcgga gcagaatcgg
    16621 tggcggtttc cgctttcccg gttttgccaa atacccgcgc cacttcaggt acgctcataa
    16681 ttagcttgtc ggttttttgc agcatactcg ccgcctctgc tgcggaaatc cccggcagcg
    16741 tcgatggcat atacaacaag tcgccttcat tgatctgcgg taaaaattcc ccgccaactt
    16801 tattgagcgg ccagagaacc gtcagcaccg aaagcgctgc caccaacagc gtggttttcg
    16861 gccagtgcag tactttcagc aacagcggat gataaacacg aatcaaaaag cgattgagcg
    16921 ggttactgct ttccggcgga attttgccac ggatccagta acccatcagg atagggatca
    16981 ctacgatcgc cagcagcgcc gcacccgcca tcgcatacgt tttggtgaac gccagcgggc
    17041 caaacagacg cccttcctgc ccttccaggg tgaagatcgg gataaacgac agcgtgataa
    17101 tcagcagact gataaacagc gccggcccca cttcaacaga tgcattggta atcacctgcc
    17161 agcgcgtttt attatccagc gtggcgtcag gatgctggtg ctgccactct tccagccgtt
    17221 tatgtgcatt ctcgatcatg acgatagcgg catcgaccat cgccccgacg gcaatcgcaa
    17281 tgccacccaa cgacataata ttggcattca gcccctggaa gtgcatgaca ataaaagcaa
    17341 tacacaaccc cagcggcaac gaaataatcg ccaccagcgc cgagcgcaca tgccagagaa
    17401 acagcgcaca gactaccgcc accacaataa actcttccag caacttgccg ctgaggttgt
    17461 cgatggcccg gtcaatgagc tggctgcgat cgtatgttgt aactatctcc acgccttccg
    17521 gcagactgct tttcagcgtt tccagtttgt ccttcacggc ggcgatcact tcacgggcgt
    17581 ttttgccgga gcgtaggatc accaccccgc ccacaacttc gccgttcagt tcggcaatgc
    17641 cccggcgcat ctccgggcca acctggatct tcgcaacatc acgcagataa acaggcacgc
    17701 cattttcact ggcttttaaa acgatgtgat taaagtcgtc gagcgtttgt agatagccgc
    17761 tggcgcgcac catatattct gcttccgcca gttcgattga cgaaccgccc gcttcctggt
    17821 ttgaagcatc cagcgcgctt tttacttcgg cgagactgat gccatactgc gccaggcgct
    17881 gaggatcgat aaccacctga tactctttca ccacgccgcc caccgacgcc acttccgcaa
    17941 cgtcagggat ggtttttagc tcatatttga gaaaccagtc ctgcaatgag cgtaaatcgg
    18001 ccagatcgtg cttaccgctg cgatccacca gtgcatattc atagatccag ccaacacccg
    18061 tggcatctgg tcccagctcg gcgctgactc ccgcaggcag cttaccctgt acctggttga
    18121 ggtactccag cacccgcgaa cgcgcccagt acggatcggt accatcttcg aaaatgacat
    18181 acacataaga gtcgccaaac tgcgagaaac cacgcacagt cttcgcgcca ggcaccgaca
    18241 acatggtggt agttagcgga taagtcacct gattttcaac gatttgcggt gcctgaccgg
    18301 gatagctggt tttaataatc acctgcacat cggagagatc cggcaacgca tccactggcg
    18361 tgttaatgat ggtccaggtg ccccagatac tcagaaataa cgcgcccatc agcaccaaaa
    18421 aacggttcgc caccgagcga cgaataatcc attcaatcat tggttattcc ctcaatgcgc
    18481 atgggtagca ctttcagagc gcatccgctc cagtgcgcca gaaatattgg cttcggaatc
    18541 aatcaggaac aggccgctgg aaaccacctt ttcaccttcc gccagaccag agcgtaatgc
    18601 ggtgacacct tgcgatgcct ggaaaacggc aacgcgtttt ggtacaaagc gcccgtcggc
    18661 atcaacggta atcacccgct gttcgctgcc ggtatcaatc agcgcctgtg acggaatgag
    18721 cagcatcggt tcgctggcgg tattgagttg caaccaggcg ttcattcccg gttttagcgc
    18781 ctcgtcggcg ttgtcgactt ccagacgcag ctgcagcgtg cgggtcgcgg catccacgcc
    18841 aggtagtagc gtccatttgc ggatggtgag tgttttatca ggtcgcgccg gaacggtgag
    18901 cgtaaactgc gaggcatctt tcaccagcca ggcgatggac tccgggatcg cagcagtgac
    18961 ccacaccggg tccataccct gaattttcgc taccacgtta tctttggcga tattcattcc
    19021 cgcgcgcaga tcaaacgcgg tgatcacgcc atcaatgggt gctttgagcg taaagcgagt
    19081 ctggattttt tgcgtggcga tcaggcggcg aatatccgcc tccggcattc ccgccagtcg
    19141 cagccgctca agaatgcctt cggtctgggt cgccgtaccg ccggtttcgc gcagcagtaa
    19201 atactcactc tgcgcttcca cccagtcggg aatggtcagg tcgagaagcg gcgcgccctt
    19261 ttgtacttta tcgcccacgg taagcggata caccttgtcg ataaacccgg cggcgcgagc
    19321 ctgcacaatg gcatactgat actcgttgta actgacattc gccgggaaac tctgggcaaa
    19381 agtcagcggc ccgcgcgtga cggtagccgt tttcaccccc agattctgcg tctgagtcgg
    19441 gtcaatgcgc acaccagacg cagaactctc ttcatcggca tatttcggca ccagatccat
    19501 atccataaac ggcgatttac ctggtttatc gaaccgcgta ttgggataca tcgggtcgta
    19561 ccagaataag attttacgtt ctgcggtcga cgttttttct gcaggcggtt ccgcctttgc
    19621 aaaccaggta aaacctgccg cagaaataat accgcccgcg atcatgctgc cgataataag
    19681 cgcgattttt ttcatctcat taaacctggg ttactggctg actttaatat cctgtaataa
    19741 agaaaggttg ccctgctgga caaaattaaa cgccactttg tcgccggttt taattccact
    19801 cattttcgtc tgcggggtga tggtaaagcg catggtcatc tccggccagt tcacggcagc
    19861 aatcggatcg tgatggatgg tgattttttt gctttccaga tcaaaacctt ttaccacgcc
    19921 ggtggcacta atgacctgtg gttgtgcttc gctcatggtt tcatgatgat gttcgttagc
    19981 ctgggcatta aagccaataa cggtaaacag actgaacatt gcgacttgca gtgctttttt
    20041 catttatttt ctcctggagt taaaaattat tgctgccaac cgccaccaag tgcggtatat
    20101 aaagaaattt cgttgacctg acgggcataa ttcaggtcaa gtacggtttg tcgggttgca
    20161 aataaagaac gctcggcatc cagcacttcc agataactta ctgcgccgtg ctgatataat
    20221 gcccgcgccc gttgcaaagt aatttgcagc gatgccagat aacgctgctg ggcgctaatt
    20281 tgatcgttca ggctttgacg taatgccagc gcatctgcca cttctttaaa ggcgttctgg
    20341 attttctgtt cataattcac taccgactgc tgctggcgaa tttcggcgat atccagattg
    20401 gcctggttgc gtccggcatt aaaaatgggg atctcaattt taggaataaa attccacatc
    20461 ccgctgctgg cgttaaataa tgacgacaga tcgctgctgg cggttgatat tccgctggtc
    20521 aggctgatag acgggaaaaa tgccgcgcgt gcagcaccaa tattggcatt agccgccatt
    20581 aacgcatgtt cagcttccat aatatccggg cgctgcaata agatttgcga cgacaagccc
    20641 gccggtaatt taacgctttg caggctgtcg ctgtttactg tctgcgcttg cggcagcttg
    20701 ccgtagcttc ccagtaacag ttgcaatgca ttattcgcct gcgccagttc cccctgacgt
    20761 ttagcgatgt cgctgcgggt actttcaatc accccgcgag cctgttccag cgccagaaca
    20821 ttactgctac ctgtcaacag ttgtttttcg acaaacgcat atgactgctg ataattacgc
    20881 agcgtttctt cggctatttg caattgcgca tacgccagct gctgattgaa atagctttgc
    20941 gcgacattag aaaccagcag aatatgcacc gcgcgctgag cttcctcagt ggctaaataa
    21001 ttttgtcgct cggcttcgct catgttcttt aagcgcccga aaaaatcgag gtcaaagctg
    21061 gcgttaaggc cagtagaaaa ctcccgcgtc gtggctgaat cgcctttaag attgccgctc
    21121 cagctgccgc tgccctcacc attgagctgt gggtagcggt cggcatcagt cagacgatat
    21181 tgcgcccgtg cctcctgcac tttcagcgtc gccatgcgca aatcccggtt attctccagc
    21241 gcctcgctaa tcagcgtctt cacctgatta tcaacaaaaa aggtgcgcca gcccgcgttc
    21301 tgatagttat ctgctgcgtt aaccaggccg ttctggctga gtgagaactg ctgcggtacg
    21361 ggcattgccg ggcgctgata atccggtgcc agtgaacaac cggttagcgc aagggccaca
    21421 caaaatggca gaagtttaca aggagacata ggctcataat ttctggtgat tttataccgc
    21481 caactttact cgccaggctc tgattttccg gtgacaggaa aatgacaaaa ttgtcatttt
    21541 gccaataagc gattgccatc tgatcccgct actctagaat tgcccgggca acatgcggag
    21601 gaaatatgaa actgttgatt gtcgaagatg aaaagaaaac cggagaatac ttgaccaaag
    21661 ggttaaccga agccggtttt gtggtcgatt tggccgacaa cgggctgaat ggctaccatc
    21721 tggcgatgac cggagattat gacctgataa tcctcgatat tatgctgccg gacgtgaacg
    21781 gctgggatat cgtgcgcatg ttgcgctccg ccaataaagg gatgccgatt ctgttgctta
    21841 ccgcgcttgg caccattgaa catcgcgtca aggggctgga gttgggggcc gatgactatc
    21901 tggtgaagcc gttcgcgttt gctgaactgc tggcgcgggt gcgcactctc ctgcggcgcg
    21961 gggcggcggt gattatcgaa agtcagtttc aggttgccga tttgatggtc gatctcgtca
    22021 gccgcaaagt cacccgcagc ggcacgcgca tcactctgac cagtaaagag tttactctgc
    22081 tggagttttt tctccgccat cagggcgaag tgctgccccg ctcgcttatc gcctcgcagg
    22141 tatgggacat gaattttgac agcgacacta acgccattga tgtggcggtg aagcggctac
    22201 gcggcaaaat cgacaacgac tttgagccga agctgattca aaccgtgcgc ggcgtgggtt
    22261 acatgcttga ggtgccggat ggtcagtaag ccatttcagc gcccgttttc gctggcaacc
    22321 cgcctgacct tttttatcag cctggccacc atcgcggcgt ttttcgcctt tgcatggatc
    22381 atgatccact cagtaaaagt gcattttgcc gagcaggata ttaatgattt aaaagagatt
    22441 agtgccaccc ttgaacgcgt actaaatcac cctgacgaaa cgcaagcccg acgcttaatg
    22501 acgctggaag atatcgtcag tggttattcc aatgtgttga tttccctggc agatagccac
    22561 ggtaaaacgg tgtatcactc ccccggtgcg cctgatatcc gcgagtttgc gcgcgatgcc
    22621 atacccgata aagacgcgcg gggcggcgag gtgtttctcc tctccggccc gacgatgatg
    22681 atgccaggcc acggtcacgg gcatatggaa cacagcaact ggcggatgat cagtttgcca
    22741 gttggcccac tggttgacgg caaaccgatt tatacgctct acatcgcgct ttcgatcgat
    22801 tttcatcttc attacataaa tgatttgatg aataaactta ttatgaccgc atcggtcatc
    22861 agcatcctga tcgtctttat cgtgctattg gcggttcata aaggtcacgc gccgatccgc
    22921 agcgtcagcc gtcaaatcca gaatatcacc tcgaaagatc tcgacgtccg ccttgacccg
    22981 cagaccgtgc cgattgagct ggaacagctg gtactgtcgt tcaaccatat gatcgagcgt
    23041 attgaggatg tctttacccg ccagtccaat ttctcagcgg atatcgccca cgaaattcgc
    23101 acaccaatta ctaatctcat aacgcaaacg gaaatcgccc tcagccagtc gcgcagccag
    23161 aaggagctgg aagatgtgct ctactctaat ctcgaagagc tgacgcgaat ggcgaaaatg
    23221 gtcagcgata tgctgtttct cgcccaggcc gataacaacc agttaatccc cgaaaagaaa
    23281 atgctcaacc tggcggatga agtcggcaaa gtgttcgatt ttttcgaggc tttagcggaa
    23341 gatcgcggcg tggagctgca gtttgttggc gacgagtgcc aggttgcggg cgatccgctg
    23401 atgctgcgtc gggcgttaag caacctgctt tcgaacgccc tgcgttatac gccacccgga
    23461 gaggctattg tagtgcgctg ccagacggtc gatcatctgg tgcaagttat cgtcgaaaac
    23521 cccggtacgc ccattgcgcc cgagcactta ccgcgattgt ttgaccgttt ctaccgcgtt
    23581 gacccctccc gccaacgaaa aggcgaaggt agcggcattg ggctggcgat agtgaaatca
    23641 attgttgtcg cgcataaagg cacggttgcg gttacgtcaa atgcgcgggg gacaaggttt
    23701 gtgattgtgt tgccggagag ggggtgatta ttaagttcaa attcagggcg agggtttacg
    23761 gcgtttttcg ctaaatttcc atcattataa acaagccacc tatcatataa agcaatgaat
    23821 aattaaatcg cgattaa
//
LOCUS       DAZFAB010000072        23654 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZFAB010000072 DAZFAB010000000
VERSION     DAZFAB010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23654
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.072"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..1763
                     /gene="adeD"
                     /locus_tag="ACHJWA_003151"
     CDS             <1..1763
                     /gene="adeD"
                     /locus_tag="ACHJWA_003151"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFO4309698.1"
                     /translation="NSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLINGG
                     EISGPIVIKGRYIAGVGAEYTDAPALQRIDAHGATAVPGFIDAHLHIESSMMTPVTFE
                     TATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCDVN
                     GASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGLGG
                     KELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNSPQ
                     CMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGLLA
                     PGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESAKLAQSAPPYGNTIARQPVS
                     ASDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFERNDVCFIAVLERYGQRLAP
                     ACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNGQV
                     QSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPALKL
                     TSQGLFDGEKFAFTTLEVTE"
     gene            complement(1809..3200)
                     /gene="uhpT"
                     /locus_tag="ACHJWA_003152"
     CDS             complement(1809..3200)
                     /gene="uhpT"
                     /locus_tag="ACHJWA_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFO4309699.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3338..4657)
                     /gene="uhpC"
                     /locus_tag="ACHJWA_003153"
     CDS             complement(3338..4657)
                     /gene="uhpC"
                     /locus_tag="ACHJWA_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFO4309700.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVIAIAAGISALLLLPFLNAQTPREA"
     gene            complement(4667..6169)
                     /gene="uhpB"
                     /locus_tag="ACHJWA_003154"
     CDS             complement(4667..6169)
                     /gene="uhpB"
                     /locus_tag="ACHJWA_003154"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFO4309701.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFSLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIEESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWLQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLTISCLHGTRVSVSLPQRYV"
     gene            complement(6169..6759)
                     /gene="uhpA"
                     /locus_tag="ACHJWA_003155"
     CDS             complement(6169..6759)
                     /gene="uhpA"
                     /locus_tag="ACHJWA_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFO4309702.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(6921..8024)
                     /locus_tag="ACHJWA_003156"
     CDS             complement(6921..8024)
                     /locus_tag="ACHJWA_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4309703.1"
                     /translation="MNSYIKIDQTGSNKFKITDLYREFNGETKCYDKKCRDMDFSGRN
                     VKEFIRLLSDHDQFEKDQISELTVAANALKLEVAKNNYNMKYSFDTQTERRMIELIRE
                     QKDLIPEKYLHQSGIKKLKLHEDEFSSLLVDAERQVLEGSSFVLCCGEKINSTISELL
                     SKKITDLTHPTESFTLSEYFSYDVYEEIFKKVVFTPGECDLVRDSLVQDGIKPEKIAK
                     IISCLSSRTGIRNLFYACSQAFHVANDNAALDVRRAATSVGDTIDVCPSFVALNGRNY
                     HQHVDFSEYGFTQTLCVLGAVVNNANDTFDVANYIYTTKGENIIHNDAEDDSPYVHSD
                     ESYRHKDDYWARDNDIRNLRRNFSGNITIATYK"
     gene            complement(8632..9114)
                     /locus_tag="ACHJWA_003157"
     CDS             complement(8632..9114)
                     /locus_tag="ACHJWA_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4309704.1"
                     /translation="MVELVCFNDDDCAQVRELLKQSNGNITDDKIDEITACLSNPQGI
                     TCFYYSPLQGYQSFLKNTSQDVSELLSKSLNGTISSTSGLKKSGNAFFGTGKYLKEID
                     FTINGFSQKMYLMAVNNVGNEVKLQCSCYFTTGSVDEEKSKSDKIDFTFSGAATTADL
                     "
     gene            complement(9262..9915)
                     /locus_tag="ACHJWA_003158"
     CDS             complement(9262..9915)
                     /locus_tag="ACHJWA_003158"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4309705.1"
                     /translation="MASPINSGIMMANLCPSTFLDKNRNVAAELDIKNNEKKYSPGSN
                     FAKWMLQEIKRLILNIMSGSRSINTDILDYFHPMPGTENNGNRTWVAATGEDEYIEIK
                     QTGDKSFNITLVGRDKPSRKEIPYSGVAVATIIKSLSEKTSALETHSADTVLRKKLVN
                     SIVMKNTDFNYEIPAGILSNIYDLLKLRIKKDEGYVPVQESFKRTDVFFDSMIMDAH"
     gene            complement(10178..10468)
                     /gene="ilvN"
                     /locus_tag="ACHJWA_003159"
     CDS             complement(10178..10468)
                     /gene="ilvN"
                     /locus_tag="ACHJWA_003159"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO4309706.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(10472..12160)
                     /gene="ilvB"
                     /locus_tag="ACHJWA_003160"
     CDS             complement(10472..12160)
                     /gene="ilvB"
                     /locus_tag="ACHJWA_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFO4309707.1"
                     /translation="MASSGTSSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAVAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYKQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(12266..12364)
                     /gene="ivbL"
                     /locus_tag="ACHJWA_003161"
     CDS             complement(12266..12364)
                     /gene="ivbL"
                     /locus_tag="ACHJWA_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFO4309708.1"
                     /translation="MATSMLNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            12929..13018
                     /gene="tisB"
                     /locus_tag="ACHJWA_003162"
     CDS             12929..13018
                     /gene="tisB"
                     /locus_tag="ACHJWA_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFO4309709.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(13142..13216)
                     /gene="ysdE"
                     /locus_tag="ACHJWA_003163"
     CDS             complement(13142..13216)
                     /gene="ysdE"
                     /locus_tag="ACHJWA_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFO4309710.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            13298..14482
                     /gene="emrD"
                     /locus_tag="ACHJWA_003164"
     CDS             13298..14482
                     /gene="emrD"
                     /locus_tag="ACHJWA_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFO4309711.1"
                     /translation="MKRHRNINLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(14490..14987)
                     /gene="yidF"
                     /locus_tag="ACHJWA_003165"
     CDS             complement(14490..14987)
                     /gene="yidF"
                     /locus_tag="ACHJWA_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFO4309712.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PHLEFDDRGDMTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRGCQACDWHLLCPHAREETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(14984..15346)
                     /gene="yidG"
                     /locus_tag="ACHJWA_003166"
     CDS             complement(14984..15346)
                     /gene="yidG"
                     /locus_tag="ACHJWA_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFO4309713.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(15336..15683)
                     /gene="yidH"
                     /locus_tag="ACHJWA_003167"
     CDS             complement(15336..15683)
                     /gene="yidH"
                     /locus_tag="ACHJWA_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFO4309714.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            15792..16241
                     /gene="yidI"
                     /locus_tag="ACHJWA_003168"
     CDS             15792..16241
                     /gene="yidI"
                     /locus_tag="ACHJWA_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFO4309715.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVERKEDVDVDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGARVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(16288..17781)
                     /gene="yidJ"
                     /locus_tag="ACHJWA_003169"
     CDS             complement(16288..17781)
                     /gene="yidJ"
                     /locus_tag="ACHJWA_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO4309716.1"
                     /translation="MKRPNFLFIMTDTQAPNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARAEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYELGEKA
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALKPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDAHFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDAQPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(17778..19493)
                     /gene="yidK"
                     /locus_tag="ACHJWA_003170"
     CDS             complement(17778..19493)
                     /gene="yidK"
                     /locus_tag="ACHJWA_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFO4309717.1"
                     /translation="MNSLQILSFVGFTLLVAIITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRMIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFLRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            19660..20553
                     /gene="yidL"
                     /locus_tag="ACHJWA_003171"
     CDS             19660..20553
                     /gene="yidL"
                     /locus_tag="ACHJWA_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO4309718.1"
                     /translation="MNGKLQTSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPIGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(20550..21872)
                     /locus_tag="ACHJWA_003172"
     CDS             complement(20550..21872)
                     /locus_tag="ACHJWA_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HFO4309719.1"
                     /translation="MTKFSVVVAGGGSTFTPGIVLMLLANQDRFPLRALKFYDNDGSR
                     QEVIAEACKVILKEKAPDIAFSYTTDPEVAFSDVDFVMAHIRVGKYPMRELDEKIPLR
                     HGVVGQETCGPGGIAYGMRSIGGVLELVDYMEKYSPNAWMLNYSNPAAIVAEATRRLR
                     PNAKILNICDMPIGIESRMAQIVGLQDRKQMRVRYYGLNHFGWWTSIEDLQGNDLMHQ
                     LRQYVSKHGYVPPQQDTHTEASWNDTYAKARDVQALDPDTLPNTYLKYYLFPDYVVQH
                     SNPEHTRANEVMEHREKQVFDACRAITAAGNSAAGKLEIDEHASYIVDLAAAIAFNTQ
                     ERMLLIVPNNGAIHNFDDEAMVEIPCLVGHNGPEPLVVGDIPQFQKGLMSQQVAVEKL
                     VVDAWEQRSYQHLWQAITLSKTVPSASVAKAILDELLEANKAYWPELR"
     gene            complement(21872..23488)
                     /locus_tag="ACHJWA_003173"
     CDS             complement(21872..23488)
                     /locus_tag="ACHJWA_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO4309720.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
BASE COUNT         5971 a         6221 c         5716 g         5746 t
ORIGIN      
        1 ataattccat taaccataaa tttcatcaca ttagccgggc ggaataccag gaattgttag
       61 ccgtttcccg tggcgacgct gttgccgatt atattattga taatgtctct attctcgacc
      121 tgattaatgg cggagaaatt tccggcccaa ttgtgattaa agggcgttac attgccggtg
      181 ttggcgcaga atacactgat gctccggctt tgcagcggat tgatgcccac ggcgcaacgg
      241 cggtgccagg gtttattgat gctcacctgc atattgaatc cagcatgatg acgccggtca
      301 cctttgaaac cgccaccctg cctcgcggcc tgacaactgt tatttgcgat cctcatgaaa
      361 tcgtcaacgt gatgggcgaa gccggtttcg cctggtttgc ccgctgtgcc gaacaggcaa
      421 ggcaaaacca gtacttacag gtcagctctt gcgtacccgc tctggaaggc tgcgatgtta
      481 acggtgccag ttttaccctt gaacagatgc tcgcctggcg ggaccatccg caggttaccg
      541 gccttgcaga aatgatggac taccctggcg taattagcgg gcagaatgcg ctgctcgata
      601 aactggatgc atttcgccac ctgacgctgg acggtcactg cccgggtttg ggtggtaaag
      661 aacttaacgc ctatattgct gcgggtattg aaaactgcca cgaaagttat cagctggaag
      721 aaggacgccg gaaattacaa ctcggcatgt cgttgatgat ccgcgaaggg tccgctgccc
      781 gcaatctcaa cgcactggca ccgttgatca acgaatttaa cagcccgcaa tgcatgctct
      841 gtactgatga ccgtaacccg tgggagatcg cccatgaagg acacatcgat gccttaattc
      901 gccgcctgat cgaacaacac aatgtgccgc tgcatgtggc atatcgcgtc gccagctggt
      961 cgacggcgcg ccattttggt ctgaatcacc tcggcttact ggcacctggt aagcaggctg
     1021 atatcgtcct gttgagcgat gcgcgtaagg tcacggtgca gcaggtactg gtgaaaggcg
     1081 aaccgattga cgcgcaaacc ttacaggcgg aagagtcggc gaaactggca caatccgctc
     1141 cgccatatgg caacaccatt gcccgccagc cagtttccgc cagcgacttt gccctgcaat
     1201 ttacgcccgg aaaacgctat cgggtcattg acgtcatcca taacgaattg attacgcact
     1261 cccactccag cgtctacagc gaaaatggtt ttgagcgcaa tgatgtgtgc tttattgccg
     1321 tacttgagcg ttacgggcaa cgtctggctc cggcttgtgg tttgctcggc ggctttggtc
     1381 tgaatgaagg cgcgctggct gcgacggtca gccatgacag ccataatatt gtggtgatcg
     1441 gtcgcagtgc cgaagagatg gcgctagcgg tcaatcaggt gattcaggat ggcggcgggt
     1501 tgtgcgtggt acgtaacggc caggtccaaa gccatctgcc gttacccatt gccgggctga
     1561 tgagcaccga cacggcgcag tcgctggcgg agcaaattga cgccttgaaa gccgccgccc
     1621 gtgaatgcgg tccgttaccc gatgagccgt ttattcagat ggcgtttctt tccctgccag
     1681 tgatccccgc gctgaaacta accagccagg ggctgtttga tggcgagaag tttgccttca
     1741 ccacgctgga agtcactgaa taataaaaaa agcccggcgt catgccgggc aaaagtcacc
     1801 agttacgttt atgccactgt caactgctga atttttttct cgcggcggat tttgcgttct
     1861 tccatcaccg ccactatcgc catcaggcag atacaaccaa tcgcggcgat atccagcgca
     1921 gcgaaggtgc ctgcccagcc ggtaaggccg aatactggcg tcccatcggc aatcattccc
     1981 agacctaact tggcaaagct gtcaccaatc aggtaagcga aggtgccttt aataccatcg
     2041 gcagcgccaa tcgctttttt aggtacaaag ccaacagcag ccacaccaat caacaattgc
     2101 gggccaaaga ccaggaaacc caacgcaaag agagaagcca gatagatata ttcgttgctg
     2161 gcatgttggt acacaccgag cgtggcgata atcagcgcca gcgcgatgca ggccaccagg
     2221 ccacggcgac cgttcgctag gtcagagagc cagccccaca gcagcgtacc gaccagcgca
     2281 ccagcttcaa acagcgtaaa gccctgaatc gccaccgctt tagagagttt cagttcctgg
     2341 aacgcgtaca cagttgacca ctggtcgata ccaatacgca ccacatagag gaaaatgttg
     2401 gcgaagcaca gcagccagat cactttgttt ttcagcacat actcaacaaa gatctgccac
     2461 ttggtcatat cggtagattc tgtctctttg tcctcttcgc tgatctcctc gccgaacagc
     2521 tcttcagctt tgccgaggcc ataagattcc ggggagtcgc tgccgtaacg caggccgata
     2581 aaaccgacaa tcagcgcgat aatcgacggg aagataaaca tgccgatgac atggccatcg
     2641 aacaggtaat ttgcgccgaa cagtgccaca ccagctgcgc ctgcaccgcc aaggttgtga
     2701 gaaatgttcc agaaaccgag gaacgtcccg cgtttacgac gcggcgtcca tttggtgatg
     2761 gtggagtaac tgcacgaacc cccggtactc tggaaaaagc cacttaaggc gtagaaggca
     2821 atcatcagga acaggctaac cgagccgctg cccatactgg cgctaaagcc cagcatacaa
     2881 atggcagaca ggatcagcat gaacggcagg aattgtttgg tgtttttgcc gtcggcgtag
     2941 taggaaacca gcgttttacc cacgccataa gtgatggaga aacccaggcc gatcatcccc
     3001 agctgcgtca tgctcaaccc gtaggtcgaa atcatatcgt tctgcgcgat gttaaagttc
     3061 ttgcgaatca ggtacatcgt caggtagccg ataaagacca ccaggtagga ttgcatgaac
     3121 ggtttgaacc acattttgcg ccgcacttcg agcggaaggt ctagggtcgg cttgcggacc
     3181 tggtttaaga aagccagcat gggttactcc tgaaatgaat acctgcgtga ggcatgcatt
     3241 gtaaaaatca gcagcaaatc aggcctgaga tagcgtccag gcaaaacctg gaaaatttct
     3301 tagttttgcc ggatatgaag tgaaaaggtg agatgcatca cgcttcgcgc ggtgtctggg
     3361 cgttcaaaaa gggcagtaac agcagtgcgg aaatcccggc ggcaatggcg ataaccacaa
     3421 aaaatccgct ccagtgccag gtatcgagca ctttcgccag cggccaacca gcaagcgacg
     3481 cccccagata agcaaacaag ccgacaaacc ccgtcgccgc ccctgccgcc tctttgtggg
     3541 aacattctgc cgccgccatg ccgattaaca tctgcgggcc aaagacaaaa aaaccaatgg
     3601 tgaagaagca ggttgcctgc atcacgtagc tggcaaatgg catcagccac agcgagccga
     3661 ctgaaagcaa aattccggca gcgaaaatca aattcatcgg cccgcggttg ccgttaaaca
     3721 gtttgtccga gccccaaccc gctaccagcg caccgataaa tccgcccagt tcaaacatcg
     3781 tcactgccgt attcgccgtg accagatcga cgcccagcgt ctcggacata tacaaattgc
     3841 cccagtcgtt gatcgccgcc cgaaccacat agaccagcac atagcaaaac gaaagcagcc
     3901 agatatacgg attcaacaac acatacttgg tgaggatctc tttacgcgtc agccctgccc
     3961 cttcttgttg ttgagcaatc tccagcgcgt cgtgtcgcca ttcaccgacc gctggtaaac
     4021 ctaacgcctg cgggcgatcg cgcagccgcc agcagagaaa aatccccacg acgatcgcca
     4081 tacaaccagc aatcatcatc ccggcacgcc agccgtaatg cagcgcagct gctgccatca
     4141 caatgggaat gagcgcaccg ccaacgttat gcgccgtgtt ccataatgcc caccaaccac
     4201 cgcgttcggt acgagaatac caggccgtta acagacgcgc acacaccggt gaaccccagc
     4261 cctggaaaaa ggcgttcagc acccagagca cggcaaacgc ccatagcgac gtcgagaagc
     4321 caaacagaat gttgatgatg cccgtggcga taagccctat ccccataaaa taacgggcat
     4381 ttgagcggtc gctgacaatg ccggagacaa acttcgacac gccgtaggta atgtaaaaca
     4441 gggtcgctaa caggccgata tcgctacggc tgagcacgcc attagcaagg atttctggta
     4501 cggcggcgtt aaaactttta cgcgtgaagt aaaacagcgc gtaacccagc cagatggtca
     4561 gcagaatatg ccgacgccag tagcgataac gggcatcaat ttcatgttta tcagtcatta
     4621 atggcgcatc ggcaggcgct ttcagaaacg gcaacatcat caaaccttag acatagcgtt
     4681 gaggtagaga aacgctgaca cgcgtgccgt gcagacagga aatggtcaag gtgccgccca
     4741 gcgccgttac gcgctcgcgc attccggtga ggccaaaacc ttgttgcccg gaacccggcg
     4801 gtaaaccgct gccatcgtct tcaatcacca gcatcaaccg ttcatcctgc agccagcctt
     4861 gcagggtgac cgcgctggca tcagcatgtt tcacaatgtt attcagccct tcctggcaga
     4921 cacgaaacag cgtcacgcgc tggttttcgc ttaacgctga ctcttcgatt cgccattcga
     4981 gatggctgac aatgccgcgc ccttccagct ccatttcccg catcagtgag cggatggcct
     5041 gctctagggt gagatcatcc aactggcgcg gacgtaaccg ccctaacaaa cggcgcaccg
     5101 cgtcgtacac gcccagcgac agttgttcga tgagctgccc gctctgcttc acactggcgt
     5161 tatctgccgc cagtcgctgg acaatgcccg cttgagtacg aatggcagtg atggtctggc
     5221 cgatatcatc atgcagctca cgcgccacat cacggcgcac gctctcttca gtttctaaca
     5281 aacgttcagc gagatgctga ttgcgcgcca gttccttttg cagcgactgg ttaagttcac
     5341 gcaaccgctg gatgccagcg cccagtaaca accctgtcag actttgcacc agcagcgaga
     5401 gcaataaatc cacaggatga tcgcgccagg tttgactggc gatcagcgcg atggcgttca
     5461 tcaatgtcgc tatcagcgcc ccttgccagc cgtagtgcca ggccagcgcg ataatcggca
     5521 gcgccaggca gaatggcgta aagcgcgaca gttcgtccgg caagcccaac tggagccaga
     5581 gactgataac aaacagcagc aagtaccaga ccagatgccg cccgcgccag ttgatcggtt
     5641 gagaaactaa cgacggcccg agcggcagcc aggtgttatt ggcgagataa tgccagaaca
     5701 ccagacatat cggggccagc gtcaggccgc cagttaaagt taacagcagc gcattccacg
     5761 actctttgcc gtgccaaagc cagggcagcg actgcaacaa cgccgccgcc gttaacgccg
     5821 caccctgtaa cagcaaggtg cgccagtcac gttgatggcg atagcgcgag atcagcgcca
     5881 cgggcagtaa cgtcagtaaa ctaccgatca tcaataagga gaaatgggtt aaaccgaccg
     5941 cctgcgttag ccagtaaatc agcagccact ccgctcccag cagcaccggc cagtatccgc
     6001 gcgggcattg cagcattagc cctagccgca gaccaaacgg aaataacagc accgccatat
     6061 cagggcgctc aaccaggtgc aggctgatac tccacaggca aaaccatgcg gcagagaaga
     6121 taaaaaagca ggcaataacg gtaattaagc gggagaacaa cgtcttcatc accagccatc
     6181 aaacatacgg cgcgccagtt caacgtcgtt actgacgccc agtttttcca tcagattggc
     6241 gcgatggacg tgtaccgttt tcggtgacaa gcccagttcg gcggcaatct ctttcaccgc
     6301 cattccttgc gccagttttt ccgccacctg ccgttcacgt ttggttagtg ggtcctggcg
     6361 accggatgcc agtttaatgg caatatccgg cgtcagataa cagccgcctg tggcaaccgt
     6421 atgcaccgca gcaatcagtt cgtcagggct acaacgcttg gagagaaagc cacgcgcccc
     6481 cgcgttaagc gcctgctcaa ccagcgccgg actgtcatga acggagagca taattgtcgc
     6541 catacctttc ggcagctggc ttagcagctc cagaccggag atatcgggca tggagatatc
     6601 gcaaatacac acctgcacac cgcgccccgg cagccccgcc agcgcctcgc gccccgaacc
     6661 aaactcggca actacttgca aatcaggttc cagccccagc aactgcgcaa agccggagcg
     6721 gacgatgagg tgatcgtcta taagggcaac ggtgatcatg attgggtcct ggcggataaa
     6781 aaacgcgcat accttaacga taagcgcgat gttgttcaag ttggatatgg ggtatgtatt
     6841 ggcttaatga tgttgagctt atcgtcaaaa cgcttacctg agaggtaagc gtttggtgcg
     6901 tgttattgag aaaaagcgaa ttatttgtag gttgcaatcg taatattgcc tgagaaattt
     6961 ctccttagat tgcggatatc attatccctt gcccaataat catctttgtg tctataactc
     7021 tcatcggaat gtacataggg agaatcatct tcagcgtcgt tatggataat attttcgcct
     7081 ttagtggtat agatataatt agcaacatca aaggtgtcat tcgcgttatt tacgactgcg
     7141 cctaagacgc aaagggtttg agtgaatccg tattctgaga aatcgacatg ctgatggtaa
     7201 tttctaccat tcaaagcaac aaaactcgga caaacatcta ttgtatcgcc aacagacgta
     7261 gcggcgcgac gcacatcaag tgcagcgtta tcgttagcaa catgaaatgc ttgtgaacag
     7321 gcataaaaca aatttctaat cccggtacga ctggataaac aagaaataat ttttgctatt
     7381 ttttctggct ttataccatc ctgtactaaa gaatctctca ccagatcaca ttcaccaggt
     7441 gtgaaaacaa ctttcttgaa tatttcctca taaacatcat aagaaaaata ttcagagaga
     7501 gtgaaagatt cggtgggatg tgttaaatct gtaatttttt tgctaagtaa ttcacttata
     7561 gtgctgttta ttttttcacc gcaacataaa acaaatgaac ttccctccag aacttgcctt
     7621 tctgcatcta caaggagtga gctaaattca tcctcatgaa gcttaagttt ttttatacct
     7681 gattgatgta aatatttttc tggaattaaa tctttttgtt cccttataag ctctatcatt
     7741 cggcgttcgg tttgcgtatc aaaagagtat ttcatgttat agttattttt agcaacttcc
     7801 agcttaagag catttgcagc aactgttaat tctgagattt gatctttttc aaattgatcg
     7861 tgatctgata ataaccttat aaactctttt acatttcgac cactaaaatc catatcccta
     7921 cactttttat cataacactt tgtttcgcca ttgaactcac gatacagatc agtgatttta
     7981 aatttattac ttccggtctg atcaattttt atatagctat tcatgcctgt aacggcaatc
     8041 tatgtcttat aattcgcatt ctccgattca ggcgtaacat gaaaataagg aaccaattta
     8101 tcactaatat catgccgata gatatgaaat gaatctaaaa gatataataa aaaacttcga
     8161 aaaatgtcac gaaaagtgtc atctttaaac cttgccctat aattatcgag caggccgctt
     8221 tgttctatca tattaaattt ataaatatta tcctcgagac ttttgtcgat actctccctt
     8281 agactctgat tttgaaactg gctattatca ttctctgttt ccgttggata tgaataactc
     8341 gtcacactaa ccatagtaac ctcgcaatat gacactttgc atattttgac attgttagaa
     8401 gtatcaatgg ctagcatgat aaatatctca atgctaaccg ctatatttca tttatttcaa
     8461 taataagact atagtgccgt taatttttat cgatttcccc aatgcaattg catcactaat
     8521 actacttatt cactatcaaa aatcgctcat tttttcaaaa agactgttta cacacagacg
     8581 gttaacaata cgtgttcacc ttaatgataa acgcgcttta tcaagaaaaa atcataaatc
     8641 agcagttgtt gcggcaccgg aaaatgtaaa atcgatttta tcgcttttgg atttttcttc
     8701 gtcgacacta ccggtagtaa aataacaaga gcactgaagc ttcacttcat ttccaacatt
     8761 attaaccgcc atcaaataca tcttctgact aaacccattt atagtaaaat caatttcttt
     8821 aagatacttc ccagtcccaa aaaaggcatt tcctgacttt tttaaacccg atgttgatga
     8881 aatggtacca ttcaaactct ttgagagtaa ctcgctaaca tcttgagatg tattttttaa
     8941 aaaactctga tagccttgca aaggagaata ataaaaacat gtaatcccct gagggttgga
     9001 taagcaagcg gttatttcat caatcttatc atccgtaata ttaccgtttg attgttttaa
     9061 taattccctt acctgagcac aatcatcatc attaaagcaa accagttcaa ccatttgttc
     9121 aagtgtgttt tgactattct ttggatcttt ttcaattatt tcaattttgt ctgtaaatat
     9181 tttaagaata tcttcactta actcaacacc gcagcaagtt attttagtag cacccttttg
     9241 ttgactttgt tcttttatag ttcaatgagc atccataatc atggaatcaa aaaaaacatc
     9301 agttctttta aaactttcct gaacaggtac atacccttca tcttttttaa ttcgcagttt
     9361 taataaatca tatatattac ttaaaattcc tgcaggaatt tcgtaattaa aatccgtatt
     9421 cttcataaca atactattaa ctaatttctt cctcaaaacc gtgtcagcgg aatgtgtttc
     9481 taacgcagaa gttttttcag acaacgattt aataatagtc gctacagcca ctcctgaata
     9541 tggaatttct tttcgtgaag gtttatctct gccaaccagt gtaatattaa aagacttatc
     9601 acctgtttgt ttgatttcga tatattcatc ctctccagtt gccgccaccc aggtacgatt
     9661 accattattt tccgtgcctg gcatagggtg gaaatagtca agaatatcgg tgttaatact
     9721 ccgagaaccg gacatgatgt ttagtattaa gcgctttatt tcctgcaaca tccattttgc
     9781 gaaattactg ccaggtgaat attttttctc gttattttta atatcaagct cagcagcgac
     9841 atttctattt ttatccaaaa aggtggaagg gcagagattt gccatcatta ttccgctgtt
     9901 tataggactt gccataatat acctcacgat gaaccattaa ttaatagaat gctggtcatg
     9961 accactggta tagacagcaa tcagaattta acaccatcgc tgaaaatttt agccctaact
    10021 gtccccccca ggttaccggt gctgcgactt aaattaacgt cctgggctaa taaattgtgc
    10081 ctgagatggt ctctggcaca atttcaccct atatatctgc acggatctat caaaaatcgc
    10141 tcatcagtaa ctcccccgag cggtttaatg tcgctcctta ctgaaaaaac actgcaatct
    10201 tgttaaacat cgtcggatcg gactgattac gctgcacttt caccacatct tccagcttat
    10261 cgatttggct tatcatctgc tccagacgct ggtcgtcatt gaccagtagc cagatatggc
    10321 ttttgtcgct gtcctgaatc ggcagacaaa gaatgccttc aacgttaaaa gcgcggcggg
    10381 caaaaaggcc acaaacgtgg gtcattacgc ccggatggtt gcgaacggtg agttccagaa
    10441 tcacgttgtc atgagttgtg ttttgcatgg cttattcccc caccatttct gtattcgccg
    10501 cacctggcgg caccatcggg tacacttttt cttcggcatc aatgcgcaca tggatcagcg
    10561 ccggaccagg gcgattgatg atttcctgca atgcagcctg cggatcggct tcgttattca
    10621 aatcacaggt ttcgaggccg aatccggcgg caatctgcat aaagttgatt tttcccggat
    10681 aggtagcggc aaaaacgccc tgcttgtaga acagactctg ttgctgatgc accagcccca
    10741 gcgcttcgtt gttcatcaga atgattttaa catccagctg gttttcgctg gcggtcgcca
    10801 tctcctgaat attcatcatc aggctgccat cgccggagaa acacaacact ttgcgatccg
    10861 ggttcgccag cgccgcgcca atcgccgcag gcaggccaaa acccatcgtg cccagcccgc
    10921 cggaggtcag ccactggcgt gggcgattga gcggataagc ctgcgccgtc cacatctgat
    10981 gctgacccac atcggtggtg ataatcgcat tgtcatcgac acaggcggca acggcgttga
    11041 tcaggccgta atggcttaac ggatcgcacg ctttcgggat tggacacggg aattcgcgct
    11101 gcaaatccgc taccaactgg tgccactctg cacgcggttg cgcttccacc tgcgggatca
    11161 gctgcgccag cacgtcatca acatccgcct gaatcgctac gtgcggctgc ttgattttac
    11221 ccagctctgc gcggtcgata tcgacatgaa taattttggc attcgggcag aactgctcgg
    11281 ttttgccaat cgcccggtca tcaaaacgcg caccgagcac aatcaacaaa tccgcctcct
    11341 gcaaaatata gttggtgctg cgcacaccgt gcatccccaa catacccagc gacaacggat
    11401 gcgcttttgg caacatgccc agcgccatta aagtcattgt ggtaggcagc tgcgctttct
    11461 ccgccagttc acgcactctc gcaggcgcat tgatcacacc accgcccaga taaagcaccg
    11521 ggcgtttggc agcattaatc attgccgctg cgtcacgaat gctttcttcg ctaaaggcgg
    11581 gcgcgacggc tttttctgcc accgcgggct gtgcttcaat ctcaaaaacg gccgtttgca
    11641 catccttagg aatgtctatc cacaccgggc cagggcggcc tgattgcgca atacggaagg
    11701 catcgctcat gacctgcggg agttcttcga tatgtctgac cagatagttg tgtttggtga
    11761 tggggataga gatgccgtag gtgtcgactt cctggaaggc gtcagtgccg atcattgagg
    11821 cgggaacctg accagtgatg caaatcagcg ggatggagtc cagccgcgca tcagcaatgg
    11881 cggtcaccag gttagtcgcc cccggtccgc tacaggccat acagaccgcc ggtttaccgt
    11941 cggtgcgcgc cattccctga gcgataaacc ccgcgccctg ttcatggcga gccagaatat
    12001 ggcggatttg cgtgctttgg cttaaggcat cgtaaacagg caggatagaa ccgcccggaa
    12061 tgcccgtcac aatcttaatg ccctgctgtt ccaggaaatg aacgataaat tctgcgccgg
    12121 taaagcgctt acgcgtcgat gatgtgcccg aacttgccat gctccagtcc ttttcttctg
    12181 ggccgacttt ccgggaggtg cttaaacgaa aaaccccgcc cggtttgcgc cggcggggtt
    12241 ttggaatcgt gtgttgttcc agtccctacg gcgcattgcc gacgaccacc accacacgca
    12301 cgacgaccac tgcggcggat ggcgcagttg gtagtagttt tgcgttgagc atggaagtag
    12361 ccattgggga ccttgttggt tttgtgttta acaatattta tacaagcaca gctttacagg
    12421 ggagacaatg gaaaattttt cagcaaggga aaattgaggg gttgatcacg ttttgtactg
    12481 tactgcaggt aacaaaaaac cccgccggag cgaggtttcg tcagccgccc gcggctggta
    12541 accgcaaagc acactgtatt atgtcaacac agaaagtata cgtgttccgc gcaaaacgcg
    12601 caatttcggc acgaattttg acgtatttag tgcatagttg agtatcgatc acagtttgcg
    12661 ttttgtccaa atattactgt ttatttatac agcaagcttc tataatatca ctgtacgcaa
    12721 tgtgttatgc gggggccgca tcgttacccg gcgcactaag tcctggctga aacgggtggt
    12781 gccgtcagcg ccttaacccc gcgtgagcac actgtgttat gtcaacgagc acaacgtttc
    12841 tccttgagat accgcgtgca caacagctgg caacaggcag cggaaaggta cgtcagctgg
    12901 cagtgctcct gaaccacagg agacgcgtat gagcctggtg gatatcgcca ttcttatcct
    12961 caaactcatt gttgcagcac tgcaactgct tgatgctgtt ctgaaatacc tgaagtaatt
    13021 cagattcaag tcgcactaaa ggggagcggg taaccgctcc cattttatat ttagcgtgtg
    13081 ggttggtgtc ggatgcgatg ctgacgcatc ttatccgacc taccatccct cccggcaaca
    13141 tttattgccg cttttgttta catattctgc cgctaaacaa ttccccattc ctggcgtata
    13201 tctggctaac atttatcaat atgatagatt cctctcccgc atttatggga atgcgtagtg
    13261 acttattcta attattttta taaaagcatc cgtgataatg aaaaggcata gaaacatcaa
    13321 tttgttattg atgttggtat tactcgtggc cgtcggtcag atggcgcaaa ccatttatat
    13381 tccagctatt gccgatatgg cgcgcgatct caacgttcgt gaaggggcgg tgcagagcgt
    13441 aatgggcgct tatctgctga cttacggtgt ctcacagctg ttttatggcc cgatttccga
    13501 ccgtgtgggt cgccgaccgg tgatcctcgt cggaatgtcc atttttatgc tggcaacgct
    13561 ggtcgcggtc acgacctcca gtttgacagt attgattgcc gccagcgcga tgcaggggat
    13621 gggcaccggc gttggcggcg taatggcgcg tactttgccg cgtgatttat atgaacggac
    13681 acagttgcgc cacgctaaca gcctgttaaa catgggaatt cttgtcagtc cgttgctcgc
    13741 accgctaatc ggcggtctgc tggatacgat gtggaactgg cgcgcctgtt atctcttttt
    13801 gttggtactt tgtgccggtg tgaccttcag tatggcccgc tggatgccgg aaacgcgtcc
    13861 ggtcgacgca ccgcgcacgc gcctgcttac cagttataaa acgcttttcg gtaacagcgg
    13921 ttttaactgt tatttgctga tgctgattgg cggtctggcc gggattgccg cctttgaagc
    13981 ctgctccggc gtgctgatgg gcgcggtgtt agggctgagc agtatgacgg tcagtatttt
    14041 gtttattctg ccgattccgg cggcattttt tggggcatgg tttgccggac gtcctaataa
    14101 acgcttctca acgttgatgt ggcagtcggt tatctgctgc ctgctggctg gcttactgat
    14161 gtggatcccc gactggtttg gcgtgatgaa tgtctggacg ctgctcgttc ccgccgcgct
    14221 gttctttttc ggtgccggga tgctgtttcc gctggcgacc agcggcgcga tggagccgtt
    14281 ccctttcctg gcgggcacgg ctggcgcgct ggtcggcggt ctacaaaaca ttggttccgg
    14341 cgtgctggcg tcgctctctg cgatgttgcc gcaaaccggt cagggcagcc tggggttgtt
    14401 gatgacctta atgggattgt tgatcgtgct gtgctggcta ccgctggcga cgcggatgtc
    14461 gcatcagggg cagcccgttt aagctcacgt caccgcagca tcgtcatcag ctccatggga
    14521 gaacgatgct gctttatcag atcacgcatc acccgcatat gcggcgcaga gtaagaataa
    14581 aacgcctgat agcccgcaca aagcacgctg tctggcgtct cctcccgagc gtgcggacac
    14641 agcagatgcc agtcgcacgc ctggcagcca cggcgcggtt gcgggttttc gctgccacac
    14701 catttttgta atgtggcgtc aaaaagctca atcgagatac gactgatatc tgcccgcacc
    14761 caacactcaa acagggcaat taaaaatttc ccccacagct ctgctgtcac cgaagcggcc
    14821 gtcatatcgc cccggtcatc aaattcaaga tggggaataa gctgtacgga gcggatgccc
    14881 cgttgtttga aattgtgata aaaatcggca ggttgcagag catccttgta ctctacaacc
    14941 agtttatgcc tgtcctcttc agcatcaatg acctgacttc ctgtcatgcg actctctcaa
    15001 taaatacgat aagttgatgt atatgcgtta cagcaaacag tattgcgaga gataacaccg
    15061 cgagggagat caaaaatttg tcacggacta cgtgaaattg ggaaaaatcg ctattcgtga
    15121 catccattaa attgcgatta cgagtgtagt gccagaggat cagcgccacg atggcgagga
    15181 tgccaatcga aatccagaat aacatgcccg cctggtgcca gttgtgtttg atagccaacg
    15241 ccatcaacgc gccgtagccc agcatggtac gaaaccacgc cagtgatgta cgctccggct
    15301 gcagccctgg gtcggcaatg cgtctggctt tgcggctatc cggcatacaa caccagtccc
    15361 ataacgatca ctgctacgac catcaatatt aagctgatga ttaataagct gttggtgtag
    15421 ggcaggtcct ctttcagacg catcgctttt tcattgcgta accagcgcaa gtagccatac
    15481 atcgctaacc cgccggaaaa cagacacaac aacaacgcca gcagttcgcg aatgacgggc
    15541 gtggcgaaat ctggcgcaag ttgatcaagc ccaacccccg ccgccagaaa tcctagtgca
    15601 gtacggatcc acgccagaaa agtacgctca ttagccagcg agaagcggta atcaggcgct
    15661 tctccgaggc gggaaatttt cattgttgct cctttgcaat ttcactaacg ctctaccttt
    15721 agcggttttt tttaagaatt tcgtccgccc gtttcctgat atgcttttgc catccgctac
    15781 aaggaggtca gatgggtatc atagcgcaaa ataaaatcag ttcattgggc atgctattcg
    15841 gtgccatcgc tttaatgatg gggattatcc acttttcatt cggccctttt tccgcaccac
    15901 cgccaacgct tgaaagcatc gtggcggaca aaacggctga aattaaaaga ggtcttctcg
    15961 ccggaataaa aggcgaaaaa attaccaccg tggagagaaa agaggacgtg gatgtcgata
    16021 aaatcctcga tcaaagcggt atcgcactgg ccattgctgc gctactttgc gcatttattg
    16081 gcggtatgcg taaggaaaat cgctggggca ttcgcggcgc gcgggttttt ggcggcggga
    16141 cgctggcttt ccataccttg ctttttggta tcggcatcgt gtgcagcatt ttgctgatat
    16201 tccttatctt ttccttcctg actggcggtt cactggttta agcagcctga ataatgcccg
    16261 ccgggtgttc atcagcgggc attatcgtca gaacttctgt tttttctctt ccaccttcac
    16321 cccttgtgtc ggcaggccgg tgtcatagtc gcgtaccact ggcgaataac catcttgtgg
    16381 gcgtggacga aatgccccca tccagcgcgg ctgtgcatct ttacgccacg gacgcaggct
    16441 ccattggtaa ctgcggaacg gatcgcgaat tttgtccata taatccaata aggcatcatg
    16501 cattttgctg cgaacgtcgg caaaatgggc atcatcgatc aggttatgca tttcatttgg
    16561 atcattacgg cgatcgtaaa gttcatcact ggtgaagagg ttgagcacca gtttaaagtc
    16621 atccgtcacc cagcaacgca ccggaataaa accgccaaag ctgtcatgct caatctcgta
    16681 gcggttaaat tccaccatca cgccgcgtgg ctctttcact gcaaggatat tttcccccgg
    16741 cagaatctct ggtttttcaa tatctgccag cgccatcatt gtcggcagta aatcgatatg
    16801 actgactggc gtatcgacct gtcgccgctc cccttgcggc gaacggatga tcagcggaat
    16861 gcgggtgatg tcgtcataca tcgccgcccc tttactgatc agcttatgtg cgcccatcat
    16921 ttcgccgtga tcggaggtat aaataaccca cgtattttca cgttgctctg gctttaaggc
    16981 attgatgacc cgtccgattt ggtcatcaac aaagtcatta caggcaaaat agagcggatg
    17041 gtgataaagc ccgtcatcac cgactggcga tggcatcgcc tgcgcccata agcggtgatg
    17101 ttctggtttg ttcgccaggt catcctgtgc tttctcgccc agctcgtagt aaaaatcagc
    17161 gtatttctct aaatactcca ccggacaggt gaacgggtga tgcggctcat catacgaaac
    17221 caccatcagg aagggttcct cggcgcgcgc gggctgttgc agaaaatcca ccgctcgatt
    17281 gctgatgcgg tgcgcccagg tgaaggtttc gtcaatatgg ttcgcctgta aatcttcgac
    17341 gctgtttagg ccattgcgcc acaggctaat ctctttttcc gtcagttcgc taagatagtt
    17401 cgccccatcg aaccagtaat cagcgtccca ctccggcgga cactcgccag tgccgaaata
    17461 gtcatgaccg tcgagatgcc atttgccgat gtaacaggtg tgatagccgg catccttaaa
    17521 gtagcgcccc atagtggaga tgtttttacc cggcgcgacg ttgttggtcc acggcccgga
    17581 ctggttagcg tagataccgg taaacagccc ggcgcgcgcc ggtgtacaaa ccggtgaaca
    17641 ggtgtaggcg gaattaaagc gaataccttc cgccgccaga ctatcaatat tttgcgtatt
    17701 cagcggttta ccgctatagc aaccgaccat attgggggcc tgggtatcgg tcatgatgaa
    17761 cagaaaattg gggcgtttca taggctatcc ttcgcgtcgg gagtaaaggt tacggctggg
    17821 tcgtgacgat gccgccagct gtcaaaaatc aggtagacaa tcactacggc ggcaatgaaa
    17881 taactgatca tcgctaacca gcgcgtaccg tagccgccga attcagccag cccggcatag
    17941 acgccaatca tcgcgaacag gatgccaatt gacgcgatct tgacgttttt ccacggtttc
    18001 atgtccaccg caaacgcatc tttgaaggtg aacggcgtgg cgcgcggttt gataaaaccg
    18061 atcaccagca tcacgaccac gttgatgcag aacgtacagg ccagcacata gaggaaatgg
    18121 aagtcgaact tcaccagata gttgatggtg atgtagctga ttatgccaat ccccatcgcc
    18181 acttttgccg ccagcgccgg gatgcgcagg aagaaaaagc ccataatgat gatggtaacc
    18241 agcggcacgt tgtaaatgcc gttgagctgt ttcatccagc tatacagccc ctgcggcgcg
    18301 ttggcgatcc acggcgctac cagcaccgaa acgatagcga taaagaaacc aaatttgcgc
    18361 ccgacggtga ccagctgctg cggctcggca ttctggttaa tgatgcgacg gtaaataccc
    18421 atactgaata acgtactggc gctattcaga aagccgttga aggtactgat cactgcacca
    18481 aataacaccg cgccgaagaa acccaccagt ggcactggca gaacgttatt gaccagcgtc
    18541 gggtaggcca tgtcggcttt cggtagatcc tgatacagat gaaacgcaat caaccctggc
    18601 agcaccagta ccagcgggtc gagcattttc agcaccgccg ttaacagcgc ccccttttgc
    18661 ccttccgcca ggctttttga cgccagcgtg cgttgcacga tgccctgatt tgtacaccag
    18721 taaaaggtgt tcaccagaat taaaccggta aatgccgcgc caatcggcaa gggatcggtc
    18781 gggccaccga ctgagtttaa tttctcggcg tgaacggtgg taatttgctc aatgccctgc
    18841 ataaagctgc ccttgcccat cgcgatcaag ccaaataccg gcaccatcaa cccgccgata
    18901 accagcccaa taccgttgat ggagtccgcc actgccattg cgcgcagtcc gccgatcacc
    18961 gcatacaaaa ttcccgccag accaagcaaa attaccagta gccagatagc cgcaccgtga
    19021 gaaatctgta gcgattcccc gacgtgaaac aggctgttga gcgccaacgc gccggagtag
    19081 agaacaatcg gcagaaagca gacgccggtg gcaatgagga agcagaagtc gatgatcata
    19141 cgcgtcgttt tatcataacg ttcctccaga aaatcgggga tggtggcaat gccgcgcttc
    19201 agatagcgcg gtaaaaagat tagcgcgagg aagatcagcg tcaccgctga agtcacttcc
    19261 cagcccatca ccgacatgcc gcttttgtag gcctgcccgg aaagaccaac cagttgttcc
    19321 gtggaaaggt tggttaacat taacgaagcg gcaataaccg gcgcttttag tgaacgtccg
    19381 gcaagaaaat agccttgttg cgatccggta tctgttttgc gaaccttcca ccaggtgatg
    19441 atcgccacca gcagcgtaaa accgacaaaa ctcaagattt gtaacgaatt catctcatag
    19501 cccttattta tgtttatgtc ccggacgcat gaatataggg agaggtaaaa tgcgtcgggt
    19561 caggcagtca gattcttaaa atacaaacgt cgtatccctg aacggattgt tttctgttaa
    19621 gttcaggttg tgagcataat tcgccggagg gatattaaaa tgaatggaaa attgcaaact
    19681 tcagatgtaa aaaacgaaac tccatacaat attcctttat tgattaacga aaatgtgatc
    19741 tccagcggaa tttctctgat ctcgctctgg catacctacg ccgacgagca ttaccgggtg
    19801 atctggccac gggacaagaa gaaaccgctt atcgccaact catgggttgc ggtgtatacc
    19861 gtacaaggat gtgggaagat tcttttaaag aatggcgaac aaataacact gcatggcaac
    19921 tgtattatat ttttaaagcc aatggatatt cactcttatc actgtgaagg tttagtctgg
    19981 gaacaatact ggatggaatt tacccccacc agtatgatgg atattcccat cggtcagcaa
    20041 agcgttattt acaatggcga aatttataat caggaactca ccgaagttgc tgagttaata
    20101 acttcaccag aagcaataaa aaataatctg gcagtcgctt ttctgacgaa aattatttat
    20161 cagtggattt gtcttatgta cgcagacggt aaaaaagatc cacaacggcg gcaaattgaa
    20221 aaattaattg ccactttaca tgccagtctg caacaacgct ggagcgtagc cgatatggct
    20281 gccacgatcc cctgtagcga agcctggttg cgtcgtctgt ttttacgcta taccggaaag
    20341 acgccgaaag aatattacct cgatgcgcgt ctggatctgg cgctatcgct attaaagcaa
    20401 caaggaaact cggttggcga agtcgctgat acgctcaact tcttcgactc ctttcatttc
    20461 agcaaagcct ttaaacataa atttggttat gcgccttcag ccgtgctaaa gaatacggac
    20521 cagcacccaa cggatgccag tccacacaat taacgtaact ctggccagta cgctttgttg
    20581 gcctccagca actcatccag aatagctttg gcgaccgagg cgctcggtac cgttttcgac
    20641 aacgtaatcg cctgccacag gtgctgatat gaacgctgtt cccaggcgtc cacgaccagt
    20701 ttttccaccg ccacttgctg actcattaac cctttctgaa actgcgggat atcgccgacc
    20761 accagtggtt ctggtccgtt gtgcccgacc agacacggga tctcgaccat agcttcatca
    20821 tcaaagttat ggattgcccc gttgttaggc acaatcagca acatccgctc ctgagtgttg
    20881 aaggcaattg ccgccgccag atcgacgatg tatgacgcat gttcgtcaat ttccagcttg
    20941 cccgccgctg aatttcctgc cgccgtaatg gcgcggcaag catcgaaaac ctgtttctcg
    21001 cgatgttcca ttacctcatt cgcgcgagta tgttcagggt tggaatgctg aacgacataa
    21061 tccgggaaga gataatattt cagataggtg ttcggcaatg tatccgggtc cagtgcctgg
    21121 acatcccgcg cttttgcata ggtgtcgttc cagctcgctt cagtatgtgt atcttgctgc
    21181 ggtggaacat aaccgtgctt agagacatat tgccgcagct ggtgcatcag gtcgttgccc
    21241 tgcaaatctt caattgatgt ccaccagcca aagtggttca ggccgtagta gcgcacgcgc
    21301 atctgtttgc gatcttgcag cccaacaatt tgcgccatcc ggctttcaat accgattggc
    21361 atgtcacaga tgttgaggat tttcgcattc gggcgcagac gacgcgtggc ttctgcaaca
    21421 attgctgccg ggttggagta gttgagcatc caggcgtttg gtgaatattt ttccatataa
    21481 tccaccagtt ccaggacgcc gccaatggaa cgcatgccgt acgctattcc gccgggtccg
    21541 caagtttcct gaccaacaac gccgtggcgc agcgggattt tttcatccag ttcgcgcatc
    21601 gggtatttgc cgacgcggat gtgcgccatg acaaaatcaa cgtcgctgaa cgccacttca
    21661 ggatcggtgg tgtaactaaa cgcaatatcc ggcgcttttt ctttaaggat gactttacag
    21721 gcttcggcaa tcacttcctg ccgcgaacca tcgttatcat aaaatttcag tgcacgaagc
    21781 gggaaacggt cctgattcgc caggagcatc aacacgatgc ctggcgtaaa ggtgcttcca
    21841 ccgcctgcga caaccactga gaatttggtc attatactgc ctccgtaatg gcaacatttt
    21901 ctgctgattg atgagaatta attaagctat cgagctgttc acgcagctgg gatacatgca
    21961 gaccaatgat cacctgaatg gcatcgccac tacggaagac gccgtgcgct cccagctttt
    22021 taaagacttc gtcatccagc gtttgtgaca tgtcatgcag tgcaatgcgt agacgcgtcg
    22081 cacaattgtt aatgctggag atattgccga ccccgcccag ggcttgcagg ataccggcag
    22141 cctgatccag ctcttttttt ggctctgccg cggtggtttg gcctcgcgag gctttgtatt
    22201 cggcttttga gtagagtttc acttccgcat cttcacgtcc cggcgtgcac atgttgaatt
    22261 gcagaatcag ggtgcggaaa accacgaagt acagcagggt aaagcacaac ccaatggcga
    22321 tttgggtcag catcatatcc gcgtggttgc tgaacatcgg gatccagttt tgcggtaaaa
    22381 cctggtcaat cagacctccg cccatgttgc ccaccacacc aaagagatac atcacggtcg
    22441 acattgaggc cgcaagcaca gcgtgtaccg caaacagcaa cggtgaaatg aacaggaagg
    22501 taaattccag cggttcggta attcccacca gcatggcggt taaggttgcg ggaatcagca
    22561 aacccgccac ttttacccga ttttccggtg cggcagtgaa gtacatcgct aaagaaatgc
    22621 ccacggcacc aaagattttt gagttaccgt gcagggcaaa acctccttcc gggaacaacg
    22681 atttcagcgg ctcggcgctc aaactgaact cttgcagatg ctgcgcccag tacatctgaa
    22741 ttccgccttc aacagctgcc ggaccaaaga taaacggtcc gtagatgaag tggtgtaaac
    22801 cggttgggat cagaatacgt tcgaggaagg tgtaaaccca caccccaagc gcacccgccg
    22861 aacgcaggaa cgcttgcaga gattcaatcc ccatttgtac ttttggccag ccgagcaatg
    22921 tcagccaggc acaggggatc atcaccagga aggcgataat caccacataa gacgtcccct
    22981 ggaaaatgcc gagaaaaacc ggcagttttt tatcgaacag acggttatgc agcgccgtca
    23041 caatgccgga aatgataatt gcgccgataa tgctggtatc gagggtttta atcccggcca
    23101 tcattgtcag accgctacct gccaccgcgt cctgagtgaa atcgacgccg aagtagcttc
    23161 cccaggtcat tcccatcgca ttgatgaaat agttccaggt caggaaactc accatcaccg
    23221 ccagacaagc acgcccctgc gcttgcttag caaggccaat gggtaaaccg acagcaaaaa
    23281 tcagcggcat attacggaat accgtccaac cgccctcttc aataatgtgt acgatttgcg
    23341 cgaataaact gttcggatcg gtcagtgatt ccccgacaaa catcgggttt tgcagcaaga
    23401 tggcaagacc caccacaatc ccggcgaagg gaaacagcag cactggcgtg aacatcgcgc
    23461 cgccaaagcg ttgaatttga ctgagcattt ccacatcctc ttttctcaat tctgtaagta
    23521 ggaagcctga ttggttatgc ggcaggcctg gagcgaggat aggaagatgc gatcggcaga
    23581 acatgacgac ccaatgggat tcgtgatcgc tttcatgctt ttcgttgggc ggtagagatc
    23641 tactttttcg cgaa
//
LOCUS       DAZFAB010000073        23294 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZFAB010000073 DAZFAB010000000
VERSION     DAZFAB010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23294
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.073"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..1137
                     /locus_tag="ACHJWA_003174"
     CDS             <1..1137
                     /locus_tag="ACHJWA_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFO4309721.1"
                     /translation="TNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSN
                     DVSIFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLS
                     SYNADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALL
                     CLASLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHD
                     VLTNIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVD
                     IIQQSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQ
                     MTISIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
     gene            1318..2808
                     /gene="dgcJ"
                     /locus_tag="ACHJWA_003175"
     CDS             1318..2808
                     /gene="dgcJ"
                     /locus_tag="ACHJWA_003175"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFO4309722.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSILQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKLINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            2851..3354
                     /gene="yeaK"
                     /locus_tag="ACHJWA_003176"
     CDS             2851..3354
                     /gene="yeaK"
                     /locus_tag="ACHJWA_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFO4309723.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGADFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(3355..3459)
                     /gene="yoaI"
                     /locus_tag="ACHJWA_003177"
     CDS             complement(3355..3459)
                     /gene="yoaI"
                     /locus_tag="ACHJWA_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFO4309724.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            3629..4075
                     /gene="yeaL"
                     /locus_tag="ACHJWA_003178"
     CDS             3629..4075
                     /gene="yeaL"
                     /locus_tag="ACHJWA_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFO4309725.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIATGLVSLIVGKQ"
     gene            complement(4032..4853)
                     /gene="nimR"
                     /locus_tag="ACHJWA_003179"
     CDS             complement(4032..4853)
                     /gene="nimR"
                     /locus_tag="ACHJWA_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFO4309726.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     YTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RAMVEMMAKEPVEWGALGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPWQ"
     gene            4950..6131
                     /gene="nimT"
                     /locus_tag="ACHJWA_003180"
     CDS             4950..6131
                     /gene="nimT"
                     /locus_tag="ACHJWA_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFO4309727.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            6186..6533
                     /gene="yeaO"
                     /locus_tag="ACHJWA_003181"
     CDS             6186..6533
                     /gene="yeaO"
                     /locus_tag="ACHJWA_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFO4309728.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYSAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(6555..6809)
                     /gene="yoaF"
                     /locus_tag="ACHJWA_003182"
     CDS             complement(6555..6809)
                     /gene="yoaF"
                     /locus_tag="ACHJWA_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFO4309729.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRLDHPAGEK"
     gene            6992..8017
                     /gene="dgcP"
                     /locus_tag="ACHJWA_003183"
     CDS             6992..8017
                     /gene="dgcP"
                     /locus_tag="ACHJWA_003183"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFO4309730.1"
                     /translation="MSDQIIARISQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERVEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNQKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHSAPHS"
     gene            8050..8148
                     /gene="yoaK"
                     /locus_tag="ACHJWA_003184"
     CDS             8050..8148
                     /gene="yoaK"
                     /locus_tag="ACHJWA_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFO4309731.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            8151..8225
                     /gene="yoaJ"
                     /locus_tag="ACHJWA_003185"
     CDS             8151..8225
                     /gene="yoaJ"
                     /locus_tag="ACHJWA_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFO4309732.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(8284..8532)
                     /gene="yeaQ"
                     /locus_tag="ACHJWA_003186"
     CDS             complement(8284..8532)
                     /gene="yeaQ"
                     /locus_tag="ACHJWA_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO4309733.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(8679..8861)
                     /gene="yoaG"
                     /locus_tag="ACHJWA_003187"
     CDS             complement(8679..8861)
                     /gene="yoaG"
                     /locus_tag="ACHJWA_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFO4309734.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(8865..9224)
                     /gene="yeaR"
                     /locus_tag="ACHJWA_003188"
     CDS             complement(8865..9224)
                     /gene="yeaR"
                     /locus_tag="ACHJWA_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFO4309735.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(9397..10035)
                     /gene="leuE"
                     /locus_tag="ACHJWA_003189"
     CDS             complement(9397..10035)
                     /gene="leuE"
                     /locus_tag="ACHJWA_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFO4309736.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGMATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(10162..11085)
                     /gene="dmlR"
                     /locus_tag="ACHJWA_003190"
     CDS             complement(10162..11085)
                     /gene="dmlR"
                     /locus_tag="ACHJWA_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFO4309737.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            11188..12273
                     /gene="dmlA"
                     /locus_tag="ACHJWA_003191"
     CDS             11188..12273
                     /gene="dmlA"
                     /locus_tag="ACHJWA_003191"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFO4309738.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGSATTPQVADAICKIILR"
     gene            12524..14134
                     /gene="yeaV"
                     /locus_tag="ACHJWA_003192"
     CDS             12524..14134
                     /gene="yeaV"
                     /locus_tag="ACHJWA_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HFO4309739.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            14166..15290
                     /gene="yeaW"
                     /locus_tag="ACHJWA_003193"
     CDS             14166..15290
                     /gene="yeaW"
                     /locus_tag="ACHJWA_003193"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFO4309740.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            15346..16311
                     /gene="yeaX"
                     /locus_tag="ACHJWA_003194"
     CDS             15346..16311
                     /gene="yeaX"
                     /locus_tag="ACHJWA_003194"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFO4309741.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTPVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(16365..17480)
                     /gene="rnd"
                     /locus_tag="ACHJWA_003195"
     CDS             complement(16365..17480)
                     /gene="rnd"
                     /locus_tag="ACHJWA_003195"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFO4309742.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(17562..19247)
                     /gene="fadD"
                     /locus_tag="ACHJWA_003196"
     CDS             complement(17562..19247)
                     /gene="fadD"
                     /locus_tag="ACHJWA_003196"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFO4309743.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTLVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVSPGQPGELCVRGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(19452..20033)
                     /gene="yeaY"
                     /locus_tag="ACHJWA_003197"
     CDS             complement(19452..20033)
                     /gene="yeaY"
                     /locus_tag="ACHJWA_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFO4309744.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQATGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGHGGWGWYNPGPARVQTVVTE"
     gene            complement(20073..20768)
                     /gene="tsaB"
                     /locus_tag="ACHJWA_003198"
     CDS             complement(20073..20768)
                     /gene="tsaB"
                     /locus_tag="ACHJWA_003198"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFO4309745.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(20826..22736)
                     /gene="yoaA"
                     /locus_tag="ACHJWA_003199"
     CDS             complement(20826..22736)
                     /gene="yoaA"
                     /locus_tag="ACHJWA_003199"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFO4309746.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            22868..23212
                     /gene="yoaB"
                     /locus_tag="ACHJWA_003200"
     CDS             22868..23212
                     /gene="yoaB"
                     /locus_tag="ACHJWA_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO4309747.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         5711 a         5941 c         5664 g         5978 t
ORIGIN      
        1 actaacaaac aaattttacc aacactcatt ttaagccttg catttttaag tggccttatc
       61 tatttagttg aaaccattgt aattatccat aaaccaatta acggcagtac actgatccag
      121 acaaagtcga atgatgtttc tattttctat attttccgcc aactcagttt tatttgttta
      181 acctcgctgg cgctcttttg ttatggaaaa gacaacatcc ttgacaacaa taagaaaaaa
      241 actggcattc tgttgctggc gctgatccct tttttagttt ttccccttct ggcacacaat
      301 ctgagcagtt ataacgctga ctattctttg tatgtcgtcg attactgtcc ggacaatcat
      361 actgcgacct ggggaatcaa ctatacaaaa atattggttt gtctgtgggc atttttactg
      421 ttctttatta tcatgcgtac acgattagcc agcgaactat ggccgttaat agcattatta
      481 tgtctggcat cgctatgctg caacttactt ctactgactc tggatgagta taattatacc
      541 atctggtata tcagtcgcgg gattgaagtc tccagtaaac tgtttgttgt gtcttttctg
      601 atttataaca tttttcagga gctgcaactc tccagcaaac tggcagttca tgatgtgctg
      661 accaatattt ataatcggcg ctactttttc aacagcgtag agtcattatt gtcgcgacct
      721 gttgttaagg acttctgtgt catgctggtt gatattaatc agttcaaacg catcaatgcc
      781 caatggggac atcgtgtggg tgataaagtg ctggtttcaa ttgtcgatat tatccagcaa
      841 agcatccgcc ccgatgatat tttagcgcga ctggagggtg aggtgtttgg cttgctattt
      901 accgaactca atagtgccca ggcaaaaatc attgcggaac gtatgcgtaa aaatgtcgaa
      961 ctcctgaccg gctttagtaa cagatatgat gttcctgaac aaatgaccat cagtattggc
     1021 acagtttttt caacgggtga cacgcgtaat atctcgcttg tcatgacgga agcagataaa
     1081 gccttacgcg aagcgaaaag cgaggggggc aacaaagtga ttattcatca tatttaagtg
     1141 caaaaatatt cagagccatg ctttttgcgt ggcttttgca tacaatttat tactattacc
     1201 cctaaatttc ccctccccag cggcgcagag atgagtataa ttagcgcccc tgtgccaggc
     1261 cgcaatcgaa ctttatctgg ttttctcgtt tcactaaccg aaggagtgcc atttatcatg
     1321 aaattgcacc atagaatgct ccggcatttt atcgccgcaa gtgtcattgt gctgacatct
     1381 tccttcctta tttttgaact tgtcgccagc gacagagcaa tgagtgccta tctgcgctat
     1441 atcgtgcaga aagcagactc ctcctttctt tatgataagt atcagaatca gagtattgcc
     1501 gcgcatgtga tgcgcgctct cgctgctgag cagtcggaag tgtcgccaga acagcggcgc
     1561 gccatctgcg aggcttttga gtctgccaat aatacccatg gcttaaacct gactgcccat
     1621 aaatacccgg gcttacgcgg cacattacaa accgcatcca ctgactgcga cacaattgtg
     1681 gaagctgcag cactattacc cgcttttgat caggcagtgg aaggcaaccg ccaccaggat
     1741 gattacggtt caggtcttgg gatggccgaa gagaaatttc actattatct cgatctcaat
     1801 gaccgctatg tctattttta tgagccggtt aatgttgaat actttgcgat gaataactgg
     1861 tccatcctgc agtcaggaag tattggcatc gatcgcaaag atattgaaaa ggtatttacc
     1921 gggcgtaccg tattgtcgag catttaccag gatcagcgta ctaaacagaa cgtgatgagt
     1981 ttgctgacgc cggtgtatgt tgcagggcag ctaaaaggga ttgtgctgct ggatattaac
     2041 aaaaacaatc tgcggaatat cttttacact catgaccgcc ctctcctctg gcgttttctc
     2101 aatgtcacgc taaccgatac agattcgggg cgcgacatta tcatcaacca gagcgaagat
     2161 aatctgttcc agtatgtcag ttacgtccat gacttaccgg gcggcattcg tgtctcgtta
     2221 tccattgata ttctttactt tatcacgtct tcgtggaaaa gcgttctgtt ctggattttg
     2281 acggcgttaa ttttgctgaa tatggtgcgg atgcacttcc gcttatacca aaatgtgtcg
     2341 cgagaaaata ttagtgatgc gatgactgga ctgtataatc gcaaaatttt aacccctgaa
     2401 ctggagcagc ggttgcagaa actggtgcaa tccggttctt cggtgatgtt tattgcaatt
     2461 gacatggaca agttaaagct aataaatgac accctcggtc atcaggaagg ggatttagcg
     2521 attacgttgt tagctcaggc gattaaacaa tcgattcgta aaagtgatta tgccatccga
     2581 ctcggtggcg atgaattctg catcattctt gtcgattcga cgccgcaaat tgcagcacaa
     2641 ctgcctgaac gtatcgaaaa acgtctgcaa catatcgcgc cgcagaaaga gatcggcttc
     2701 tcttccggta tttacgcgat gaaagaaaac gatacgttac atgatgcgta taaagcttcc
     2761 gatgagcgtt tatatgtcaa taagcagaac aaaaacagcc gttcatgata accttctgtg
     2821 gttgtttgct tgtaatctca ggagcgtgaa atgactgaaa tggctaaagg aagcgtgacg
     2881 catcagcggt taatcgcttt attatcacaa gaaggtgctg atttccgcgt cgttacccat
     2941 gaagcggtag gtaaatgtga agcggtgtca gaaattcgtg gcaccgctct aggccagggt
     3001 gctaaggcac tggtatgtaa agtcaaaggc aatggcgtaa atcaacatgt tctggcgatc
     3061 ctcgcagccg atcaacaggc cgatctgagc caactcgcca gccatatagg cggattacgc
     3121 gcttcgttgg ccagcccggc ggaagtcgat gaactcacag gctgtgtctt cggcgcgatc
     3181 ccccctttca gcttccatcc aaaactcaaa ctggttgccg accctctact cttcgaacga
     3241 tttgatgaaa tcgccttcaa tgcagggatg ctggataaat ccgttatttt gaaaaccgcg
     3301 gattatttgc gcattgcgca accagaactg gtcaatttcc gccgcactgc gtaactagcc
     3361 tgtccgttca ataagcagaa cggacaaaac cagcacaaca gcaatggcaa aaaacgatga
     3421 cgtgataatc agtgtctcga cgaacatttg atcgttcata gcataccccc gggagatagc
     3481 tgttttcacg ttattgttag cgtgcacaaa tggcagtttg atgacagttc gcctgatttt
     3541 ttatgcaaaa aacgtaaaga ttttctactt ccttcctgca gcaagcgtaa agtaagcagg
     3601 cttattattt tttggcaagg aaaccacgat gtttgatgtc actttgctga tcctgctcgg
     3661 gttagctgcg ctgggcttta tcagtcataa caccactgtc gccgtttcaa ttctggtgtt
     3721 gatcattgtc cgcgtcacac cgttaagcac ctttttcccg tggattgaaa aacaggggct
     3781 aagtatcggg ataatcattc tgacgattgg cgttatggcc cccatcgcca gcgggacgct
     3841 accgccctca accttgattc actcgtttct gaactggaaa tcgctggtgg caattgcagt
     3901 aggcgttatt gtctcctggc tgggtgggcg cggcgtgacg ttaatgggca gccagccgca
     3961 actggttgct gggctgctgg tgggcactgt tttaggcgta gcgctgtttc gcggcgtacc
     4021 ggttggcccg cttattgcca cgggtctggt ttcgctgatt gtggggaaac agtagttaat
     4081 cccgcaatat atcggcctgg cgtttgcccc aggcctttct taaacatagt aataaaggcc
     4141 gttgttgaat cataccccag cgtatgagca actttctgta cagtatcgcc tttcactaat
     4201 ccctgaagcg ccataatcag ctgtaactgc tgtcgccact ggcgaaaact caaccccgtc
     4261 tcttttacta tcagtcgggc aagattgcgt tcactcatcg caaaaaagcc agcccattgc
     4321 cccaatgccc cccactcgac gggctctttc gccatcatct ccaccatcgc gcggatttta
     4381 ggatgagaag aaacgggtag gtgcagctgt tgttgtggct gctgagggag ttcatcaaac
     4441 aggacctgaa ttaatctgcg ggtcatcggc tcagcacgct gagtcgtcgt cctgtttgcc
     4501 agagttaaaa ttaattcccg gcataagggc gatattttca atgtgtaaca tgttgtcggc
     4561 attgtcactg cgctgggttc gataaacaga aaacagagtt cagcgttagc tgtcacttga
     4621 ttgctatgtt ccaccccgcc cggtatccaa accgcatatt gtggcggcac catccacaaa
     4681 gcattttcca ccgtacaggt aattgcacca tgtagcgcca gtatcagctg tcctttgcgg
     4741 tgctgatgta caggactggt taactcatcc gttccggcgt gaatacaaaa tgcgaccgcg
     4801 gcttcatggt gtcggtcggg ttcatagcca ttgagattca acctgtgcat cattttgtcc
     4861 gaatttagcg ataatttgtc attttagctt gattcaacat aacaataaaa acggtaaggt
     4921 acagcctcgt ttgtaacaat gagaagcata tgacctgttc aacttcatta agcggcaaaa
     4981 acaggattgt ccttatcgct ggcattctga tgattgccac tacattacgc gtcaccttca
     5041 ccggcgcagc accgttactc gatacgattc gttccgctta ctcgctgacg actgcgcaaa
     5101 ccggcttatt gaccaccctg ccattattgg cctttgcgct aatctcacct ctggctgccc
     5161 cggtggctcg acgttttggt atggaacgta gcctgtttgc cgcgttactt ttgatctgtg
     5221 caggtatcgc aattcgctct ctcccttcgc cttacttatt atttggcggt acagcggtca
     5281 ttggcggtgg gattgcatta ggtaatgtct tactgccagg attaattaaa cgcgatttcc
     5341 ctcattccgt cgccagactt accggcgcat attccctgac aatgggagct gcggcggctc
     5401 tgggatcggc tatggtagtg ccgctggctt tgaacggttt tggctggcaa ggcgcgttgc
     5461 tcatgctgat gtgttttcct ctgctggctc tttttttatg gctgccacag tggcgaagcc
     5521 aacaacatgc aaatttgagt acttcacgcg ccttacatac tcggggtatc tggcgttcgc
     5581 cgcttgcctg gcaggtaaca ttgtttcttg ggatcaactc actggtctat tacgtgatta
     5641 ttggctggct tccggcgatc ctcatcagtc acggctatag cgaagcacag gcgggttcac
     5701 tgcatggttt gctgcaacta gccacagcag ctcccggttt gctgatccca cttttcttac
     5761 atcatgtgaa agaccagcgt ggtattgcag cgttcgttgc cttgatgtgc gcagtgggcg
     5821 cggttgggct ctgcttcatg ccagcgcacg cgatcacctg gactctgctt ttcggttttg
     5881 gttccggcgc aacaatgata ctggggttga cgttcattgg tctgcgggct agttctgcgc
     5941 atcaggcggc ggcactctcg gggatggcac aatccgtcgg gtatttgttg gcagcctgtg
     6001 ggccgccgct gatgggtaaa atacacgatg ctaacggtga ctggtccgta ccactaatgg
     6061 gtgttgcaat actttcacta ctgatggcga ttttcggact ttgtgccggg agagacaaag
     6121 aaattcgcta atatccggta ctatagtgac gtgacaaatc atgcgtgaaa gggagaacaa
     6181 acacgatgaa tattcagtgc aaacgcgttt atgatccggc agaacagagc gatggttatc
     6241 gcgtactggt cgaccgcctc tggccacgcg gtatcaaaaa aaccgattta gcccttgatg
     6301 agtgggataa agaaatcacg ccgtcaacgg aactgcgcaa agcctttcac ggcgaagtcg
     6361 tcgattttgc aacctttcgc gagcaatatc ttgcagaact ggcacaacac gagcaagaag
     6421 gaaagcggct ggcggacatc gccaaaaaac agccgctgac cctgctctac tcagcaaaaa
     6481 acaccacgca gaaccatgcg ctggtgctgg ccgactggct acgtagcttg tgattttagt
     6541 acagcatccg gcggttattt ttcaccagcc ggatggtcca gtcgccacaa tgcccattca
     6601 tcaatcgttt caccgcccgg taatttgcaa ttgttgctga ccccttgcgc tgtctgcact
     6661 ggaatgagcg tcccgccctt ctgctggcaa tagaccgacg ccggatttgc cataccaatc
     6721 tgcggcggtt taggtgcttc tggctgagaa ggggttgaac aaccagccag gaccagcaag
     6781 caaggcagaa caaaactgat aattttcatt tattgatctc acatatgtat ccaagcttag
     6841 agtatcgcgg tatcgttttg ttttgcagca ctatttttat tacattcact caaaacatat
     6901 tacgtcttgt ttcatctttg ttgatgatgt tttatcatgc ctgcaaagat taaataatca
     6961 gcatttaccc gccgtatcct ggagttgttt cgtgtcagat cagattatcg cccgcatctc
     7021 gcaatccctt gccaaagaac agtcactgga aagtctggtc cgacagcttc tggagatgct
     7081 ggaaatggtc actgatatgg aatcaaccta cctgaccaaa gtggatgtcg aagcgcgcct
     7141 gcagcatatt atgtttgccc gtaacagcca gaaaatgcac atcccggaga attttaccgt
     7201 ctcgtgggat tactcgttat gcaaacgcgc cattgatgaa aactgctttt tcagcgatga
     7261 agtccctgac cgttggggcg actgtattgc ggcacgcaat cttggcatca ccacatttct
     7321 gagcacgcca attcacttac cggatggatc attctatggc acgctttgcg ccgccagcag
     7381 tgagaagcgc cagtggagtg aacgcgtgga acaggtttta cagttattcg ccggactgat
     7441 tgcacaatat attcaaaaag aggcactggt tgaacagctg cgcgaagcca atgctgcgct
     7501 gattgcgcaa tcgtataccg actcgttaac cgggctaccg aatcggcggg cgatttttga
     7561 aaatctgacg acgctgtttt ccctcgcccg gcatcttaac caaaagataa tgatcgcgtt
     7621 tatcgatctg gataacttca aattaatcaa tgatcgtttt ggtcataata gtggcgatct
     7681 gtttctcatt caggttggcg agcgccttaa tacgctccag caaaatggcg aagttattgg
     7741 tcgtctcggc ggtgatgagt ttttggttgt ttcactaaac aacgagaatg cggatatttc
     7801 gtcgctgcga gaacgcattc agcagcaaat acgcggagaa tatcacttag gtgatgttga
     7861 tttgtattat cctggtgcca gtcttggcat agtagaagtc gatcctgaaa cgaccgatgc
     7921 agacagtgcc ctacatgctg ccgatatcgc gatgtatcag gagaaaaaac acaaacagaa
     7981 aacacctttt gtcgcgcatt cagcgccaca ttcctgaggc gtattcacat ccttttgatt
     8041 ggtgataaca tgcgaatcgg tattattttt ccggttgtaa tcttcattac agcggtcgta
     8101 tttttagcat ggttttttat tggcggctat gctgccccgg gagcataaag atgaaaaaaa
     8161 caacgatcat tatgatgggt gtggcgatta ttgtcgtact cggcactgag ctgggatggt
     8221 ggtaacgtca catctaaaaa atagcaaagg ctgcctgtgt gcagcctttg tgcaatttac
     8281 gcgttaactt ttaatcttcc tgtagataaa tagcacgaca atcgcaccaa taacggcaac
     8341 cacgaagctg ccaaaattga agccatcgac tttaccaaag ccaaacagcg tgctgatcca
     8401 accgccgact acggcaccga ctatccccag caggatagtc ataaagaatc caccaccatc
     8461 tttacctggc atgatccact tcgccagaat accggcaata agcccaaaaa taatccatga
     8521 cagaatgccc attgtttcct cacttatctg ttttgcatta gcgggttagt cgctgataaa
     8581 aagcatagca caacatcggg agggcaagat ttgtgacgag catcacggag gtttttttgc
     8641 gatggcgcag aaattgcgcc atcaacgatc agtgataatt accaaccaca aacatcatgt
     8701 tcgttttccg tgtcataaga acgcacggta ttcaccagat cttttatcac ttcagccgcc
     8761 acttctggca ccagcaaagt catcggcgtc tctgtttcat aatcgacaga aacgccattg
     8821 ctgttattgg tgacggtcac ggtatacgtt gctttgccca tgattcattt cccgttatga
     8881 atgactttcc gttgttgcgc accttccatc aggacttcag gagccacgaa gaagtcaatg
     8941 ttgaaataag tatcgtcagt catggcttca atgttgtgcc acttttctgg agggaacacc
     9001 gcaaactgcc ccgcttcgat aaggatcacc tgatcaggct ctgcactgtg ttcatcagcg
     9061 tagccgagat atttgaccgc cccatgcata acggaaaggc gtgggtaaac ccccgggcgc
     9121 gttcctttat caagatgacg ttcgaatatt ccggcaggcg cagtttgttt attccagaaa
     9181 ggcgttgagc gcgtatgaat ataattctgt gggatttgca gcatcctttt ccctccttcg
     9241 gtgaatgcgc tgaaaacggt ttattccagc cgtttcaggg tacgcctgat aatttgcttt
     9301 ttaaatacca tttattggtt actttttagc accatatcag agaaaaatca gggaggatta
     9361 tagatgggaa gcccgcgaca acgcgggctg aaagcatcag gattgcagcg tcgccagtcg
     9421 ggcagcgaaa cccacgaaca tcaaaccaat cagtgagttg cccactttag ccagtttctt
     9481 tttggtacgt atgtactgcg tgacaaaagc accagatata atcaggaagc tcaaatagca
     9541 gaaactcacc agttccagcg tcgtcgccag aataaagaat gaaattcccg tatgtggggc
     9601 attaacatcg ataaactgta cgaaaaacga cacatagaac aaaatggctt tcggattagt
     9661 caggctcaaa attaacgcgc gtttaaaaat agcaccgtat tggggctcat cggatttggc
     9721 ctcgttattt ttacccttca gggtcgcgta aagaatttta ctccccagat agagcaaata
     9781 aaacgcacca agataacgta caatgttgaa taatatcggg gtggtcttaa ttaatgtcgc
     9841 cattccagcc catgccagaa acatcaatac cgcatcgcca ataaatacac cgcaggccgc
     9901 aagataaccg cctttcatac cgctactgac gctatttttg agtacaaaca gggtatttgg
     9961 ccctggcacc aacacaataa aaatggcccc aaccagatag gtccagtaat tcagaacccc
    10021 gtattcagcg aacacattaa cctctttaat tatctttcga tcatgcgcga ttaaaggtga
    10081 atatgctaac caatctgtag cggcttagaa aggagaaaat caggttttaa cctgatatca
    10141 acccgataat tgaatcatta atcaggcatg ctccagtgaa aaattcgggt agtgctcggc
    10201 aaaatactgg cgtaaaaatt ctaccgttat ccgcactttc gctgacgtcg ccagccttga
    10261 aacataaacg gcccagacgt tcgctggctg gtaatattcc ggtagcactt gcactaaatg
    10321 accactggca atgttttcgc taacatccca ccaggaacgc agagcaatcc cctgcccatc
    10381 gagacaccac tgatggacaa tttctccatg attagacgac agtgggccgg tgactttaat
    10441 cgcatggtgg ccttctttat tgcgtaattg ccagacacca aaaggatgat cgcgctcttt
    10501 aatgaccagg cacggcaatg cagaaagatc ggttaaatgt ttcggcgcgc catgttgcgc
    10561 aataaattcc ggcgacgcgc agagaatgcg gtaattggtc gccagtttgc gggcgattaa
    10621 attaggggcg atgtcatcgc caatgcgaat atcgagatcg acaccttcat tgaccaaatc
    10681 gaccagccgg tcttccacat caaaacgtaa ttccagttgc ggatacgcct tcgccagcgc
    10741 tgataacgcc ggagccacca cctgtcgtcc aaaaccaaag ctgctgataa tacgcagcat
    10801 cccctgtggc acctgacgca cgtcagaaag ttcgtccatc atctgaccga catcctgcaa
    10861 aatccgctgc gcccattcat aaatccgctc tccttcttcg gtaatggtga cgcggcgggt
    10921 ggtgcggtgt aacaacacca cgtttagcgt ttgctccagc aaggcgatgc gcttgctgac
    10981 gaacgccggt gaaacgccca gttcttccgc cacggcggca aaaccagccc ggcgagccac
    11041 cagcataaag acgcgcaaat cattcagcag cggtaaatta ttcatgattc gtgttttatg
    11101 tttcaccagt tacggggatt aattcctttt cagtcaatta taggatggtg atgttgtcaa
    11161 ttttgatggt caggaagtga gaacccaatg atgaaaacga tgcgtattgc tgcgatcccg
    11221 ggagacggga ttggcaaaga agtccttcct gaagggattc gcgtgttaca ggctgccgct
    11281 gagcgctggg gcttcgcctt gagttttgag caaatggagt gggcgagctg cgagtattac
    11341 agccatcacg gtaaaatgat gccggacgac tggcatgagc aacttagccg tttcgacgcc
    11401 atctattttg gtgccgtcgg ctggccggac accgttccgg accatatttc gttgtggggt
    11461 tcgctgctga aatttcgtcg tgaattcgac cagtacgtca acctgcgccc ggttcgtctc
    11521 tttcctggcg ttccctgccc gctggcggga aaacagcctg gcgacatcga tttttacgtg
    11581 gtcagggaaa acaccgaagg cgaatattcc tcgctcggcg gtagagtgaa tgaaggtaca
    11641 gagcatgaag tcgtcattca ggaatcggta tttacgcgtc gtggtgtcga tcgcattttg
    11701 cgttatgcct tcgaacttgc gcaaagccgc ccacgtaaga cgctaacttc tgccactaaa
    11761 tcaaacggtt tagccatcag catgccgtac tgggatgagc gagtggaagc aatggccgag
    11821 aattacccgg agatccgctg ggacaagcag catattgata ttctctgcgc gcgttttgtg
    11881 atgcagccgg aacgcttcga tgtggtggtg gcgtccaatt tgtttggcga tatcctttcc
    11941 gatcttggcc cggcctgcac cggcaccatt ggcattgccc catccgccaa cctgaatccg
    12001 gaacgcactt tcccctcgct cttcgagcct gtccacggtt ccgcgccgga tatctacggg
    12061 aaaaatattg ctaaccctat cgccacaatt tgggccgggg caatgatgct cgattttctc
    12121 ggcaatggcg atgagcgttt ccagcaagcg cataacggta ttctggcagc gattgaagaa
    12181 gtgattgctc acgggccgaa aacaccggat atgaaaggca gtgccaccac gccacaggtt
    12241 gccgacgcga tttgcaaaat tattttgcgt taaggtcaaa ccagtttatt tgaaccgcgt
    12301 cactgacgcg gttttgttat tcgttctttg cagttaataa cctgcgtcat ttcacctttt
    12361 attgtttccg tttcgtgttt tatggctttc cgtattctta attgtttaat ttatgtaaca
    12421 tgcaaataat caattcgtta aacacgacat acaactcatt accctgcaaa taattacaac
    12481 tggcaaaagc cagagacggg cttttatgcc caggagatga ctcatgatga gcaatgttaa
    12541 gaaaaaagat gtgccgctga taagcatcag cctggtggcc attcttttca tcgcagctgc
    12601 attaagcctt ttcccacaac aatcggccga cgcggccaac gcaatataca cttttgttac
    12661 gcgtacgtta ggttccgcag tacaggtatt ggttttgctg gcaatgggac tagtgattta
    12721 tttagccacc agtaaatacg gcaatattcg tcttggcgaa ggaaaaccgg aatacagcac
    12781 gctctcctgg ctgtttatgt ttatttgtgc cggtttaggt tcttctacgc tttattgggg
    12841 ggttgctgaa tgggcctatt attatcaaac gcctggatta aatatcgcac cgcgttcaca
    12901 acaggcactc gaatttagcg tcccttactc cttcttccac tggggcatca gcgcctgggc
    12961 aacttatacg ctggcctcat taatcatggc ttatcacttt catgtgcgga aaaacaaagg
    13021 tctgagtctt tccggcatta ttgccgcgat taccggcgtt cgcccgcaag gcccatgggg
    13081 aaaactggtt gatttgatgt tcctgatcgc cactgtcggc gcactgacta tttcccttgt
    13141 tgtcaccgca gcaaccttta ctcgtggact ttccgcactg accggtttac ccgataactt
    13201 caccgtgcag gcatttgtga tcctgctttc cggcggcatt ttttgcctaa gctcatggat
    13261 tggtatcaac aacggtttgc aacgtctgag caaaatggtt ggctggggcg cgttcctgct
    13321 gccattactg gtgctgattg tcggtccaac cgaatttatt accaacagca tcatcaatgc
    13381 catcggcctg accacgcaaa acttcctgca aatgagctta ttcaccgatc cgcttggcga
    13441 tggttcattt acccgcaact ggaccgtttt ctactggctg tggtggatct catacacccc
    13501 tggcgtagca atgtttgtca cccgcgtttc ccgcggtcgt aagattaaag aagttatctg
    13561 gggactgatc ctcggcagca ccgtcggttg ctggttcttc ttcggcgtaa tggaaagcta
    13621 tgccattcat cagtttatca atggcgtaat caacgtccca caggtgctgg aaactctggg
    13681 cggcgaaaca gctgtacagc aagttctgat gtcgttgcca gccggtaaat tgttcctcgc
    13741 cgcatacctg ggcgtgatga ttattttcct tgcctcgcat atggatgcag tggcctacac
    13801 catggcggcg accagtacgc gtaatctcca ggaaggtgac gatcctgacc gtgggctgcg
    13861 tcttttctgg tgcgtggtga tcactctgat cccgctttcc atcttgttta ccggtgcttc
    13921 gctggaaacg atgaaaacca ccgtcgtgct cacagccctt cccttcctcg tcattttact
    13981 ggtgaaagtc ggcggattta ttcgctggct gaaacaggat tacgccgaca ttccggctca
    14041 tcaagttgaa cattatctcc cgcagacacc ggttgaagcc ctggaaaaaa caccagtgct
    14101 ccctgcggga accgtattca aaggcgacaa ctgagcgcca tcatcctaac gataaaggta
    14161 tccctatgag caatctgagc cctgactttg tactacccga aaatttttgc gctaacccgc
    14221 aagaggcgtg gaccattcct gcccgttttt ataccgatca gaacgccttt gaacacgaaa
    14281 aagagaacgt cttcgccaaa agctggattt gcgtcgctca cagcagcgaa ctggcgaatg
    14341 ccaatgatta tgtgacgcgt gagatcattg gcgaaagcat cgtgctggta cgcggtcgtg
    14401 ataaggtttt gcgcgcgttc tataacgtgt gtccgcaccg tggtcatcag ttgttgagcg
    14461 gtgaaggaaa agcaaaaaat gtgattacct gcccatatca cgcatgggca ttcaaactcg
    14521 atggcaatct ggcccatgca cgtaactgcg aaaacgtcgc caatttcgat agcgacaaag
    14581 cgcaactggt tccggtgcgt ctggaagaat atgccggatt cgtcttcatc aacatggacc
    14641 ccaacgccac cagcgtagaa gatcagttac ccggtctggg cgcgaaagtg ctggaagcct
    14701 gcccggaagt ccacgatctg aaactggcgg cccgctttac cacccgcacg cctgccaact
    14761 ggaagaacat tgtcgataac tatctcgagt gctatcactg tggtccggcg catccaggtt
    14821 tctccgactc cgtacaggtt gatcgttact ggcacaccat gcacggtaac tggacgctgc
    14881 aatacggttt cgccaaaccg tccgaacagt cgtttaaatt tgaagagggt acggatgcgg
    14941 cattccacgg tttctggctg tggccgtgca cgatgctgaa tgtcaccccg atcaaaggga
    15001 tgatgacggt catttatgaa ttcccggtgg attctgaaac taccctgcaa aactacgata
    15061 tttacttcac caatgaagag ttaaccgacg agcaaaaatc gctgattgag tggtatcgcg
    15121 atgtgttccg tccggaagat ttacgtctgg ttgaaagcgt acagaaaggg ctgaaatcgc
    15181 gtggctatcg tggtcagggg cgcatcatgg ccgacagtag cggtagcggc atttccgaac
    15241 atggtatcgc ccatttccat aatctgctgg cgcaggtatt taaggactaa tgacatcggc
    15301 ggcggtattt tccgccgccg ggctgatttt tgatggagta cagcaatgtc agactatcaa
    15361 atgtttgaag tacaggtgag tcaggtggaa ccgcttaccg aacaggtgaa acgcttcacg
    15421 ccggtggcaa ccgatggcaa accgctgcct gcgttcaccg gaggaagtca catcatagtg
    15481 cagatgagcg atggtgataa ccagtacagt aatgcgtatt cactactgag ttcaccgcat
    15541 aacacctcta gttatcagat tgccgttcga ctggaagaaa actcgcgcgg cggttcccgc
    15601 tttttgcatc agcaggtaaa agtgggcgat cggttaacga tctcaacgcc taataacctg
    15661 tttgcgctaa ttccctcagc cagaaagcat ctgtttatcg cgggcggtat tggtatcacc
    15721 cctttcctgt cgcacatggc agagttgcaa cacagcgacg tcgactggca gctacattac
    15781 tgctcgcgaa atccagaaag ttgcgcattt cgtgatgagc tagtccagca tccgcaggct
    15841 gagaaagtcc atttgcatca ttcatcaacc ggaacacgac tggaattagc gcgattattg
    15901 gcggatatcg aacctggcac acacgtttat acctgtggcc ccgaggcgct aattgaagcg
    15961 gtaagaagtg aagctgcgcg tctggacatc gctgccgata cgctgcactt tgagcaattt
    16021 gctatcgaag acaaaaccgg cgatgcattt accctggtgc ttgcccgttc cggaaaagag
    16081 tttgtggtgc cggaagagat gactattttg caggttattg aaaataataa agccgcgaaa
    16141 gtggaatgtt tatgtcgtga aggggtatgc ggaacctgcg aaacggcaat actggaaggt
    16201 gaagctgacc atcgggatca gtattttagc gatgaagagc gtgccagcca gcaaagtatg
    16261 ttgatctgtt gttcgcgtgc gaagggtaaa cgcctggtgt tggatttgta gtttgccgaa
    16321 gccggatgtg gcgctgagcg cgcccagtcc ggcttcggaa aattttactg cggatattcc
    16381 tgcaataaat tgtgtaatgc ttccgccatc agctcaccac gccagccgga aatcagctcc
    16441 ggcaaattgt tctgcggttt cagtttccag tgccagttca gcagttggtt gatttgccga
    16501 cgcgatgcca gcaattcggc gctgatcttg tgcgtttcgc tcacgtcagt aatcagcgac
    16561 ttaatcgctt taaacgcttt acgataaccc ggcatgtcca tcaggttaag catcggctgc
    16621 ggtaaggcct cttccggcaa tgtctgcgct ttttccacca gcgccagcag cgttttaccg
    16681 tgaaagcgga tttcgctacc ggataaaccc aggctgtcca gttcgcctaa acttcccggc
    16741 atataacgcg ctaccgacca caaatgctct tcacgcacga caaagttcac cgccagatcg
    16801 cgctctcgcg ccttgcgcag tcgccagtcg gctaacagtt gcagacaggc cagttggcgt
    16861 gtgcgtaatt gccaggcatt ggtgatatca cgccaggcat cttccggcgc aacgacttcc
    16921 tgacgacgca tttgcatcag gcgacattca tccagcgccg ctggtagcca gccggaggcc
    16981 tccgtttcta ccataagctt ggcggtgatc ggtaacagat accagacatc cgccgctgca
    17041 tattcacact gacgttcggt cagcggtctg gccagccagt cggtgcgcga ttcactcttg
    17101 tccagcgtaa cgccggaata ctcttccacc atggaagcga aaccccatga catcgggcgt
    17161 ccgcagaagg cagcaaggat ttgcgtgtca atcaggggtt gtggtaattc gccaaagaca
    17221 ttgaggaaca cttccagatc ttcactgcct gcatggagaa attttgtgat ggacggatcg
    17281 cgcaggatcg ctttcagcgg tgaccagtcg gtgatcccga gaggatcaat tagcgccaga
    17341 tgctcgccat cgaaaagttg aatcaacccc agctgcgggt aataagtgcg cgtacgaaca
    17401 aattcagtat ccagggctat cgccggaaag gcacggacgg cttcacacaa agaagccagc
    17461 gcatcgtccg tggtaatcat ttggtaattc aaattgtttt ctctttagtg ggcgtcaaaa
    17521 aaaacgccgg attaaccggc gtctgacgac tgacttaacg ctcaggcttt attgtccact
    17581 ttgccgcgcg cttcgtcacg taattctcgt cgcaaaattt ttccgacgtt agatttcggt
    17641 aactcatcac gaaactccac cagcttcggt actttgtagc ccgtgagctg acggcggcaa
    17701 aaggtcacca gtgactcttc ggtaagcgat ggatcttttt tcactacgaa gattttcacc
    17761 gcttcaccac tggagccgga aggtacacca acagccgcga cttcctgtac gccaggatgc
    17821 tgcatgacga catcttcaat ctcgttggga tagacgttaa aaccggaaac caaaatcatg
    17881 tcttttttac gatcgacaat gcgcaggaat ccttcttcat ccattaccgc gatgtcgcca
    17941 gtgtgtaacc agccgttttt gatgatttca tcggtagcat ccggacgctg ccaatagccc
    18001 agcatcacct gtggacctct gacgcaaagt tcacctggct gacctggcga tacttcatta
    18061 tcgtcgtcat ccaccagttt ggcttccgtc gacggcaccg gcaaaccaat gctaccgcta
    18121 tgataatcaa tatcataggg gttcacgctg accagcggcg cacattcggt caggccataa
    18181 ccttccagca gatactgtcc ggtcagtttc acccagcgtt ctgccaccac ttgctgaaca
    18241 ggcatcccac cgcctgcaga aaggtgcaga ctggagaaat ccagttgttg aaactcttta
    18301 ttgttcagca acgcgttgaa caaggtgttt acgccggtga tagcggtaaa cggatatttc
    18361 gccagctctt tgaccagccc cggaatatcc cgcgggttag tgataagcag gttctgccca
    18421 cccagttcga taaacagcag gcagttaatg gtcagtgcaa aaatgtgata cagcggcagc
    18481 gccgtcacca ccagctcttt gcccggatgc agcagaggcc catacgtagc gttaacttgt
    18541 tccaggttcg ccagcatatt gcggtgagtc agcatcgcgc ctttcgccac acctgtggtg
    18601 ccgccagtgt attgcaggaa ggctaaatct tctggcacca gttcaggttt gacatactgc
    18661 atacggtagc cgttgtgcag ggcgctacga aatgaaatgg catctggcag atgatatttc
    18721 ggcaccagac gtttgatgta tttaacaacg aagttaacca gtgtgccttt cgctgtggaa
    18781 agctgatcgc ccatacgggt caggataacg tgctgaactg cggttttatc gaccactttt
    18841 tccagcgtgt gagcaaagtt ggacacaatg acaatcgccg atgcgccgct atcgttaagc
    18901 tgatgctcaa gctcacgcgg ggtatacaac gggttaacgt ttacgacgat catcccggca
    18961 cgcaaaatgc caaacagcgc caccggatat tgcaataaat taggcatcat caacgcaacg
    19021 cgatcgcctt tcttcagccc caacccttgt tgcaaataag cggcaaacgc gcgactgcgt
    19081 tcttccagct tgcggaaggt catcacctcc cccatattca caaacgcagg ctgatcggcg
    19141 tagcgcgcga ccgactgctc aaacatatct accagagatt gataacggtc agggttgatc
    19201 tccgtcggaa cgtccgcggg ataacggtta agccaaacct tcttcaattc ttcacctcta
    19261 aaatgcgtgt tcgtcgtcat cgcaacccca aatgatatac atgccgttaa cataatatta
    19321 actcatcata ccagcttgat aattacccaa cgaaaaggtt gcgaagcgcg tcactattta
    19381 tttttatctt taccgtaaga atgcagaaac agcggaccag ccgctgtttc tttttcttta
    19441 aaaacaagca attattcagt tacaactgtt tgtactctcg cggggccggg attataccag
    19501 ccccatccgc cgtggccata gggccagcca cgaccgccat aaaaccaggg atcaatcggc
    19561 tgaggcggca taatcacctg ctgggttaaa tgccaacgtt tgtaacctgt tgcctgcatc
    19621 accataaatt tatagggcgt gttgccgatt tttccgtcaa ccgcaccagt gattggcccg
    19681 actaccgtaa ccagttgtcc acggaaatcc accgggtcca gaaaaccgtt cacatcggca
    19741 taaatgcgac cgcgagaagg ttctcccagc gtcggtctgg ctccgctgtc cagcggtacg
    19801 gtagcaattt ccaggcgggt tttcccttgc tggttttgta ccgcaaccac tttgccacca
    19861 aagcgtgcct cctgaccaac gtacagctgc ggcgcactca tcacccgaac taaatcttgt
    19921 tgcggcgtgg gactgctgcc tttaatggcg tccggcacag tgacacaacc gctcagcatt
    19981 agcgcaaacg tacctgccag tatgcctttg ataacattct tttgaaccgc catgatgcga
    20041 ctcctttttt tctcagggca tactcttaag attcattctt tgcccggaag tttcttccat
    20101 gcgacgttgt tacgtaaata aaccggttcg gcatgttcca ccgccaccgt tttaccctcg
    20161 gcaaacatct gacaagcaat cggcagcata tcttctgcag cgggcagtaa aacttctccg
    20221 tcgcgcaaaa ccagtccgct ctctttaccg agatccggcc aggcttgcca gcctgtacct
    20281 accgtcaccc attccccgga aagctgttgc attcgttcat gaacgagttc gggtttgagt
    20341 acggcttcgg tttcttcacc gtgccagata ccgttttcat cacgctgata ttcggcccag
    20401 taaacttcgc ccattcgcgc gtcaatggct gacagcacgc gtgttgcgcc gtttttgcgc
    20461 cacgcccctt gcgccatcgt cattagcgtg gagacgccaa tcatcggtaa ttccgcgcca
    20521 agcgccagcc cttgcgcgat gccaatacca atgcgcacgc ctgtaaagct accggggccg
    20581 cgcccgtaag ccagagcgtt aatatcagtc agggaagttc cgctggtggt caggatatcc
    20641 tgcaccatcg gtaagattcg ttgagtatgt tcacgagggc aaagctcaaa atgagcgttg
    20701 acagtaccgt cgttccacag ggcgacagag caggcctctg tcgcggtatc gatagccaga
    20761 attcgcatgg gtcttcgtgc ttagatcaat aaaaaggcgc gcatcatacc atactccgta
    20821 acaaattacc tggaggatgg tatcgcaagg aaacgaaccg cacgggcaat gtcacgggtg
    20881 cgtggcgcgg gcggcagact ggcgagaaac gtcgcgccgt aaggacgcat caccagccga
    20941 ttgtcacaaa ttaccaaaac cccgcgatcg tcggcgtcgc gaatcagtcg cccgactccc
    21001 tgcttgagag taatcaccgc atccggcagt tgtacttcat cgaacgggtc gccaccgcgc
    21061 aaacggcaat cttccatgcg cgcttttaac aatggatcat caggcgaggt aaacggcaat
    21121 ttgtcgataa tcaccaatga caatgtatcg ccacgcacgt ccaccccctc ccagaaacta
    21181 ctggttgcca caagaagcgc gttaccggca ctgacaaatt gctgcaacag ttgccctttg
    21241 ctggtttccc cctgcaataa tacgggaagc gtcatggtag cgcggaactg ctcggccaga
    21301 tcgcgcatca tggcgtgcga ggtacaaagc ataaaacagc gaccgttgtt agcttcgatg
    21361 atcgggcgca gcatagccgc cagttgccgc gcggaacccg gttgattggt ttgcggcagg
    21421 ttgcgcggca cacagagtaa cgcctggcgg ctgtaatcaa acgggcttgg caatagcaac
    21481 gactccgcct gttctatccc aagccgcgag gtgaaatgat gcagatcgtc gtttaccgac
    21541 agcgttgctg aggtgaagat ccagctaccg ggcttttgcg ccattaactc tttgaattta
    21601 tccgccacgc tgagcggcgt gagagccaga gtaaaatggc gcgaagtgca ttcgtaccag
    21661 tagctgtagc ccggctgatt gatctctttt agccgcttca gccgtgtgcg atacaacgtg
    21721 gcgcgctcaa atgccgcatc cagcaaggcg gaacgcccca gtgacagttt cgccacgtca
    21781 taacaaagtt ccagggtgtc atcgagcagt aaaaatgccc gctgaatttg cggattagct
    21841 aacagctcac gcaggttacc gcgataacct ggttcaccga gttgcagacg aaaatcctgc
    21901 gcactctggg caagacgatc agcgcacttt tgtaactgct gggtgtcttt taattcggtg
    21961 cggtaggcga tggtgatgtc ttttgccagg tcgagcagtt gtcgactgga gagtgactga
    22021 ccaaaatact ggctggcaat gtccggtagc tggtgggctt cgtcgaagat catgacgtcc
    22081 gcttccggga tcaactcgcc aaatccactc tctttaacca ccatatccgc cagaaagaga
    22141 tgatggttta ccaccaccac atcggcgtcc atcgcttttt tacgtgcttt gaccacaaag
    22201 caatctttat acatcgggca gtcgctgcca agacagttgt cgttggtgct ggtgaccagc
    22261 ggccacgcct gcgaatcttc cgccacgctg acgcaggtgc tgatatcacc atcgactgtt
    22321 tgattagacc aggagcgcag caggatcaca tcgcttaata tttgtaccgg aagatcgccc
    22381 cccgccagcg cctgctgttc gagacgttcg aggcagaggt agtttgagcg ccctttcagc
    22441 aacgccacgt tgcccgtgta tttcaacgcc tttgagaccg tcggcaaatc gcggctatag
    22501 agctgatcct gcaacgcttt tgagccggtc gagataatga ctttcttttt cgcccgcagc
    22561 gcaggggcca ggtaagcgta ggttttgccc gtaccggttc ctgcttccac caccagcggc
    22621 tggccttttt ctatcgcctg ggtgacggct accgccatct gtcgctgtgg ttctcgcggc
    22681 ttaaagcctg gtatcgcttt cgccagctga ccgtctggtg caaaatcgtc cgtcacacta
    22741 ccccctgttg atttgaacag ggattatgtc aggatgaggg cgctttcgcc agttgaagtg
    22801 gtgacggcga cctcacattg tggcagtctt tgcagcacga aaatggaagg ataatgagga
    22861 taaaatgatg actatcgttc gtatcgatgc tgaagcccgc tggtctgatg tagtaatcca
    22921 caacaacacg ctctactaca ctggtgtacc ggaaaacctc gacgccgatg cctttgagca
    22981 aaccgccaac accctggcgc agattgacgc cgtgctggaa aaacagggca gcaataaatc
    23041 gagcattctg gatgccacca ttttcctggc cgataaaaac gacttcgcgg cgatgaataa
    23101 agcgtgggat gcctgggttg tcgcgggtca tgcgccggtg cgctgcacgg tacaagcggg
    23161 tttgatgaac ccgaagtata aagttgaaat taagattgtg gctgcggtgt aagctttatc
    23221 gaagcaaaat aagtcagacg ataatttatc gataatactg gtcggttata cataaatcga
    23281 ccagagaata agat
//
LOCUS       DAZFAB010000074        23260 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZFAB010000074 DAZFAB010000000
VERSION     DAZFAB010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23260
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.074"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(45..1403)
                     /gene="plaP"
                     /locus_tag="ACHJWA_003201"
     CDS             complement(45..1403)
                     /gene="plaP"
                     /locus_tag="ACHJWA_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFO4309748.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(1393..1455)
                     /gene="yoeI"
                     /locus_tag="ACHJWA_003202"
     CDS             complement(1393..1455)
                     /gene="yoeI"
                     /locus_tag="ACHJWA_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFO4309749.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(1670..2598)
                     /gene="yeeY"
                     /locus_tag="ACHJWA_003203"
                     /pseudo
     CDS             complement(1670..2598)
                     /gene="yeeY"
                     /locus_tag="ACHJWA_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
     gene            complement(2644..3468)
                     /gene="yeeZ"
                     /locus_tag="ACHJWA_003204"
     CDS             complement(2644..3468)
                     /gene="yeeZ"
                     /locus_tag="ACHJWA_003204"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO4309750.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            3894..3944
                     /gene="hisL"
                     /locus_tag="ACHJWA_003205"
     CDS             3894..3944
                     /gene="hisL"
                     /locus_tag="ACHJWA_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFO4309751.1"
                     /translation="MTRVQFKQHHHHHHPD"
     misc_feature    3920..4043
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            4090..4989
                     /gene="hisG"
                     /locus_tag="ACHJWA_003206"
     CDS             4090..4989
                     /gene="hisG"
                     /locus_tag="ACHJWA_003206"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFO4309752.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDDAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTQRLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            4995..6299
                     /gene="hisD"
                     /locus_tag="ACHJWA_003207"
     CDS             4995..6299
                     /gene="hisD"
                     /locus_tag="ACHJWA_003207"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFO4309753.1"
                     /translation="MSFNTIIDWNSCTAKQQRQLLMRPAISASESITRTVNDILDSVK
                     ARGDDALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MAHQVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKVGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            6296..7366
                     /gene="hisC"
                     /locus_tag="ACHJWA_003208"
     CDS             6296..7366
                     /gene="hisC"
                     /locus_tag="ACHJWA_003208"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFO4309754.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIIAMRERVAQIIAER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            7366..8433
                     /gene="hisB"
                     /locus_tag="ACHJWA_003209"
     CDS             7366..8433
                     /gene="hisB"
                     /locus_tag="ACHJWA_003209"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFO4309755.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPQLLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTRRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            8433..9023
                     /gene="hisH"
                     /locus_tag="ACHJWA_003210"
     CDS             8433..9023
                     /gene="hisH"
                     /locus_tag="ACHJWA_003210"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFO4309756.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            9023..9760
                     /gene="hisA"
                     /locus_tag="ACHJWA_003211"
     CDS             9023..9760
                     /gene="hisA"
                     /locus_tag="ACHJWA_003211"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFO4309757.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAISCWQNA"
     gene            9742..10518
                     /gene="hisF"
                     /locus_tag="ACHJWA_003212"
     CDS             9742..10518
                     /gene="hisF"
                     /locus_tag="ACHJWA_003212"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFO4309758.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            10512..11123
                     /gene="hisIE"
                     /locus_tag="ACHJWA_003213"
     CDS             10512..11123
                     /gene="hisIE"
                     /locus_tag="ACHJWA_003213"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFO4309759.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(11220..12197)
                     /gene="wzzB"
                     /locus_tag="ACHJWA_003214"
     CDS             complement(11220..12197)
                     /gene="wzzB"
                     /locus_tag="ACHJWA_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFO4309760.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKELRIRQIQEALQYANQAQVAKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(12343..13509)
                     /gene="ugd"
                     /locus_tag="ACHJWA_003215"
     CDS             complement(12343..13509)
                     /gene="ugd"
                     /locus_tag="ACHJWA_003215"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFO4309761.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTEAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNSRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELRDVADKVYTRDLFGSD"
     gene            complement(13758..15164)
                     /gene="gndA"
                     /locus_tag="ACHJWA_003216"
     CDS             complement(13758..15164)
                     /gene="gndA"
                     /locus_tag="ACHJWA_003216"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFO4309762.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQI
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(15365..16030)
                     /gene="perB"
                     /locus_tag="ACHJWA_003217"
     CDS             complement(15365..16030)
                     /gene="perB"
                     /locus_tag="ACHJWA_003217"
                     /EC_number="2.3.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-perosamine N-acetyltransferase"
                     /protein_id="HFO4309763.1"
                     /translation="MNLYGIFGAGSYGRETIPILNQQIKQECGSDYALVFVDDVLAGK
                     KVNGFEVLSTNCFLKAPYLKKYFNVAIANDKIRQRVSESILLHGVEPITIKHPNSVVY
                     DHTMIGSGAIISPFVTISTNTHIGRFFHANIYSYVAHDCQIGDYVTFAPGAKCNGYVV
                     IEDNAYIGSGAVIKQGVPNRPLIIGAGAIIGMGAVVTKSVPAGITVCGNPAREMKRSP
                     TSI"
     gene            complement(16074..17005)
                     /locus_tag="ACHJWA_003218"
                     /pseudo
     CDS             complement(16074..17005)
                     /locus_tag="ACHJWA_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            complement(17293..18663)
                     /gene="cpsG"
                     /locus_tag="ACHJWA_003219"
                     /gene_synonym="manB"
     CDS             complement(17293..18663)
                     /gene="cpsG"
                     /locus_tag="ACHJWA_003219"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFO4309764.1"
                     /translation="MKSLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSEALKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGEMVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVKLMEKKTKAL
                     LKLLSE"
     gene            complement(18667..20115)
                     /locus_tag="ACHJWA_003220"
     CDS             complement(18667..20115)
                     /locus_tag="ACHJWA_003220"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HFO4309765.1"
                     /translation="MSDAPIIAVVMAGGTGSRLWPLSRELYPKQFLQLSGDNTLLQTT
                     LLRLSGLSCQKPLVITNEQHRFVVAEQLREINKLNGNIILEPCGRNTAPAIAISAFHA
                     LKRNPQEDPLLLVLAADHVIAKESVFCDAIKNATPIANQGKIVTFGIIPEYAETGYGY
                     IERGELSVPLQGHENTGFYYVNKFVEKPNRETAELYMTSGNHYWNSGIFMFKASVYLE
                     ELRKFRPDIYNVCEQVASSSYIDLDFIRLSKEQFQDCPAESIDFAVMEKTEKCVVCPV
                     DIGWSDVGSWQSLWDISLKSKTGDVCKGDILTYDTKNNYIYSESALVAAIGIEDMVIV
                     QTKDAVLVSKKSDVQHVKKIVEMLKLQQRTEYISHREVFRPWGKFDSIDQGERYKVKK
                     IIVKPGEGLSLRMHHHRSEHWIVLSGTAKVTLGDKTKLVTANESIYIPLGAAYSLENP
                     GIIPLNLIEVSSGDYLGEDDIIRQKERYKHED"
     gene            complement(20097..20606)
                     /locus_tag="ACHJWA_003221"
     CDS             complement(20097..20606)
                     /locus_tag="ACHJWA_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFO4309766.1"
                     /translation="MFLHSQDFATIVRSTPLISIDLIVENEFGEILLGKRINRPAQGY
                     WFVPGGRVLKDEKLQTAFERLTEIELGIRLPLSVGKFYGIWQHFYEDNSMGGDFSTHY
                     IVIAFLLKLQPNILKLPKSQHNAYCWLSRAKLINDDDVHYNCRAYFNNKTNDAIGLDN
                     KDIICLMRQ"
     gene            complement(20609..21574)
                     /locus_tag="ACHJWA_003222"
     CDS             complement(20609..21574)
                     /locus_tag="ACHJWA_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase family protein"
                     /protein_id="HFO4309767.1"
                     /translation="MNKQRIFIAGHQGMVGSAITRRLKQRDDVELVLRTRDELNLLDS
                     SAVLDFFSSQKIDQVYLAAAKVGGILANSSYPADFIYENIMIEANVIHAAHKNNVNKL
                     LFLGSSCIYPKLAHQPIMEDELLQGKLEPTNEPYAIAKIAGIKLCESYNRQFGRDYRS
                     VMPTNLYGPNDNFHPSNSHVIPALLRRFHDAVENNSPNVVVWGSGTPKREFLHVDDMA
                     SASIYVMEMPYDIWQKNTKVMLSHINIGTGIDCTICELAETIAKVVGYKGHITFDTTK
                     PDGAPRKLLDVTLLHQLGWNHKITLHKGLENTYNWFLENQLQYRG"
     gene            complement(21577..22695)
                     /gene="gmd"
                     /locus_tag="ACHJWA_003223"
     CDS             complement(21577..22695)
                     /gene="gmd"
                     /locus_tag="ACHJWA_003223"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFO4309768.1"
                     /translation="MTKVALITGVTGQDGSYLAEFLLDKGYEVHGIKRRASSFNTERI
                     DHIYQDPHGSNPNFHLHYGDLTDSSNLTRILKEVQPDEVYNLAAMSHVAVSFESPEYT
                     ADVDAIGTLRLLEAIRFLGLENKTRFYQASTSELYGLVQEIPQKESTPFYPRSPYAVA
                     KLYAYWITVNYRESYGIYACNGILFNHESPRRGETFVTRKITRGLANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVRMQWLMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKMSF
                     VGKGIEEKGIVDSVEGQDAPGVKPGDVIVAVDPRYFRPAEVDTLLGDPSKANLKLGWR
                     PEITLAEMISEMVAKDLEAAKKHSLLKSHGFSVSLALE"
     gene            complement(22715..>23260)
                     /locus_tag="ACHJWA_003224"
     CDS             complement(22715..>23260)
                     /locus_tag="ACHJWA_003224"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO4309769.1"
                     /translation="IICNQFWKHKDHATAFRAFKIYTEYNPDVYLVCTGATQDYRFPG
                     YFNELMVLAKKLGIESKIKILGHIPKLEQIELIKNCIAVIQPTLFEGGPGGGVTFDAI
                     ALGKKVILSDIDVNKEVNCGDVYFFQAKNHYSLNDAMVKADESKIFYEPTTLIELGLK
                     RRNACADFLLDVVKQEIESRS"
BASE COUNT         6221 a         5683 c         5293 g         6063 t
ORIGIN      
        1 agccaaatgt tcattcgact ccggtttatt attagaaatg atgattatgc tacgtcttcg
       61 tactgcggaa ccgggttacg gaagctcttg gtgacgcaag ccaggtaaat cagaccgata
      121 gcagcccaga tcagacccag aaccattgag ctttcttcca ggttaaccca cagcgcacca
      181 acggtcagcg caccacacat cggcaggaac agatactgga agtgatcttt cagcgtctta
      241 ttacgcttct cacggatcca gaactgcgag atgaccgaca ggttaacgaa ggtgaacgcc
      301 accaacgcac caaagttgat cagcgccgta gccattacca ggtcgaagtt gattgccagc
      361 agggcaatcg cgccaaccag gatgatgttc atcgccggag tacgccattt cgggtgtaca
      421 taaccgaaga aacttttcgg gaacacgccg tcacgaccca ttacgtacat cagacgcgct
      481 acgcctgcgt gcgccgccat accggacgcc agtacggtaa tggtggagaa gatcagcgca
      541 ccgacctgga acgctttacc cgcaacgtac agcatgattt caggctgtga cgcatccgga
      601 tctttaaagc gagagatatc cgggaagtac agctgcagga agtaagttgc aaagatgaag
      661 atcatgccgc cgatcagcgc ggtcaggaaa atcgcacgcg ggatcacgcg ctctgcatct
      721 ttggtttctt ccgacagatt gctgatgccg tcaaagccgg taaaggagaa gcacaggatt
      781 gtcgccccgg taatcatcgg gataacatgc gcatcgccag accagaatgg acgagtgctc
      841 gccagcgtac cagcgccttc accttcaaat acgccataaa caaccatgcc cagaatcacc
      901 gcgatcagca ctacctgcaa cacgacgatt acagtgttga agttcgctac ggatttcaga
      961 ctacgcaggt taaaggcggt cataaaggcc accagcgcca ccacaaacat ccacgatggg
     1021 atggaaggca ccagagcttc aaaatagatt ttcgccagca gaatgttgat catcggcgcg
     1081 aacagatagt cgagcagaga agaccaaccc accataaagc cgacagtcgg gctaatggat
     1141 ttctgggcgt aagtatatgc agagccagca gaaggatagc ggcgaaccag cttcccgtag
     1201 ctcagagccg taaacaggat cgcaatcaat gcgaacgcat aggctgtcgg cacatgacca
     1261 tccgtgaggc ctgaaacgat accaaaagta tcaaacagcg tcatcggctg catataggca
     1321 agacccatca ttacaaccgg aactaacgta agcgttttac gcaattccac gcgagaggtg
     1381 tttggagtaa cgttatgcga catggtcatt ctcctttacg gtgataaccg tcgcgtaagc
     1441 aaaaaattgc cccatttttt tggattcctc agcgacaaca actgtcgatt tttagtaaat
     1501 atctatccgg tacgaagccc ggcctcttgg tatgaatgat tggtttgaag caaaaaataa
     1561 ccgacgctga tgaaacgtcg gtttttagtc attttttgac agcggcgcat tgtgcctaaa
     1621 tgggggggaa atgacaagag aatgagaggc ttgtcataat aatttttctt taaatggctg
     1681 attttccgtc atcagatttt gcataaacac cggcgatcgc actatttgct aaaatttcat
     1741 cccgccacca gccgctggca agtcctgcgg tctgttcatt ccagccaatc cagacgggtt
     1801 caaagagagt ttgggcgacc actttcttct cgattaacgc gccactatcg agaaaacgtt
     1861 gcgccagata acggggtaaa tagccgcagc cgaggccgct aatttgcagc tccagcttgg
     1921 ttttaaaatc gaaaacggta atggcttctt gttcgtcgag aagttgtgag gccgtagaag
     1981 cgcctgcctg cgcagtgtcc cccaccacaa tcgcccgata tcgtttaatg atacgacggt
     2041 ttaatggttc ttcttcctgg gctaacggat gatgaggcgc gaccgcaaag acttgttcca
     2101 gatcgcccaa ccgcgagaaa ccaaactcac tggaagatgg tggctcatgc atcgcgccaa
     2161 caataatatc cgctcgcccc tgagtcaggg catcccatga accaccgagt acgccattga
     2221 taaacttcag gcgcgtcacg ttatgatgtt gataaaaagc ttcaatgagt ggcgcaagca
     2281 gagaaaaagg aaacgtgtcg tccacgccaa tcaccagctc gttttcccag ccttcatgca
     2341 gtttgatcgc ctgcttttcc agttctcgca cggtatgcag aacttcccgc cccttctcta
     2401 ataacatttt ccggtacggg tgaatttagc gcggtgaccg ctacgatcaa gcaattgaat
     2461 attaaggtcg ctttccagct tgtgaacggt gtaactcaga gcggacggcg tcttgtagag
     2521 tttagccgac gccgccgcaa aactgccttc tttttctaag gcatcgagga tcatcaacac
     2581 atccagcagt ggtttcatac acgccccctt gcagtgcatg cggcgtcctg ctggcgtgat
     2641 cgtttactcc agcggcatta ccagcggatc gggatactgg tattcaaatc ccagttcatt
     2701 acaaatccga ctgccatcaa taatcttacc tttgccgctg tccagactat ttctgaattg
     2761 cggaggttcc agccccagta aacgggccat ctgcggatag aaaacattac gcgcagggtg
     2821 agctggcgca catatattat agatgtgtcc gcctttaggt gcctgtaaca acagagtgat
     2881 agcgccaata acatcttcta aatggactaa attaacacca tgttcaccat caggcgcggt
     2941 ttttccggca aagaagcgtc cgggatgacg ccccggtccg accaggcccg caagacgcag
     3001 aatatcgacc gaagtaccgg gtaaattgtg cagccagtct tcgagttctt ctaatactcg
     3061 tccactgttg gtcaccggat tacgcggggt ggtttctttc accgtgcctt gcgcgtcgcc
     3121 atagacagac gttgaactgg taaaaataat tcggggaata cgatgggcca gcgcgctatc
     3181 cactaactct tgtaccgctt gtaaatagaa ctcatcgccg gggccgctac gacgggccgg
     3241 aagcgtaatg accagcgcat cggcatccat cagggcatcc agatcgtcag aatcgcaaac
     3301 taactcaggc tccatgcgaa gcagatagct atcaatgcca ctcattcggg ccgcttcgac
     3361 accatcttgt gtggttttac tcccggtgac ttgccagcct cgcgctgaaa gtgacatcgc
     3421 cagcggcatg cctaaccacc ctaacccgac aattgcgacc tttttcatcc gttatctcct
     3481 gatacacctt agatctataa ggctacgcta gcgtatcaaa actgacaatt cattctatga
     3541 atgaaatcga aactgttagc aaatatacca ttgcctgatg cgctccgctt attagtccta
     3601 caaaacatca atatattgaa tttaggcaac atttgtagac cagataaggc attcacgccg
     3661 catccggcag gaacaaagcc cactttagtt acagaacaca atgttcttga gggttttaat
     3721 gacatatttt cacgattact tttttaacct agtcactctt agccccctcc tttccccgct
     3781 cattcattaa acaaatccat cgccataaaa tatataaaaa agcccttgct ttctaacgtg
     3841 aaagtggttt aggttaaaag acatcagttg aataaacatt cacagagact tttatgacac
     3901 gcgttcaatt taaacagcac catcatcacc atcatcctga ctagtctttc aggcgatgtg
     3961 tgctggaaga cattcagatc ttccagtggt gcatgaacgc atgagaaagc ccccggaaga
     4021 tcaccttccg ggggcttttt tattgcgcgg ttgataacgg ttcagacagg tttaaagagg
     4081 aataagaaaa tgacagacaa cactcgttta cgcatagcta tgcagaaatc cggccgttta
     4141 agtgatgact ctcgcgaatt gctggcgcgc tgtggcatta aaatcaatct tcacacccag
     4201 cgcctgatcg cgatggcaga aaacatgccg attgatattc tgcgcgtgcg tgacgacgac
     4261 attcccggtc tggtaatgga cggcgtggta gatcttggga ttatcggcga aaacgtgctg
     4321 gaagaagagc tgcttaaccg ccgcgcccag ggtgaagatc cacgctactt taccctgcgt
     4381 cgtctggatt tcggcggctg ccgtctttcg ctggcaacgc cggttgatga tgcctgggac
     4441 ggcccgctct ccttaaacgg taaacgtatc gccacctctt atcctcacct gctcaagcgt
     4501 tatctcgacc agaaaggcat ctcttttaaa tcctgcttac tgaacggttc tgttgaagtc
     4561 gccccgcgtg ccggactggc ggatgcgatt tgcgatctgg tttccaccgg tgccacgctg
     4621 gaagcaaacg gcctgcgcga agtcgaagtt atctatcgct cgaaagcctg cctgatacaa
     4681 cgcgatggcg aaatggaaga atccaaacag caactgatcg acaagctgct gacccgtatt
     4741 cagggtgtga tccaggcgcg cgaatcaaaa tacatcatga tgcacgcacc gacccaacgt
     4801 ctggatgaag tcatcgccct gctgccaggt gccgaacgcc caaccattct gccgctggcg
     4861 ggtgatcaac agcgcgtagc gatgcacatg gtcagtagcg aaaccctgtt ctgggaaacg
     4921 atggaaaaac tgaaagcgct gggtgccagt tcaatcctgg tcctgccgat tgagaagatg
     4981 atggagtgat cgccatgagc tttaacacaa tcattgactg gaatagctgt actgcaaagc
     5041 aacaacgcca gctgttaatg cgcccggcga tctccgcttc tgaaagcatt acccgcactg
     5101 ttaacgatat tctcgatagc gtgaaagcac gcggtgatga cgccctgcgg gaatatagcg
     5161 cgaagtttga taaaaccacg gttaccgcac tgaaggtgtc tgctgaggaa attgccgccg
     5221 ccagcgaacg cctgagcgac gagctaaaac aggcgatggc ggtggcagta aagaatattg
     5281 aaaccttcca cactgcgcaa aaactgccgc cggtagatgt agaaacgcag ccaggcgtgc
     5341 gttgccagca agtcacgcgc ccggtagctt cagttgggtt gtatattcct ggcggctccg
     5401 ccccgctctt ctcaacggta ttaatgctgg caactccggc gcgtattgcg ggctgtaaaa
     5461 aagtggtgtt gtgctcaccg ccgccgattg ccgatgagat cctttatgcg gcgcagctgt
     5521 gcggtgtgca ggacgtgttt aacgtcggcg gcgcacaggc cattgccgcg ctggcgtttg
     5581 gtacggaatc tgtgccgaaa gtggacaaaa tcttcgggcc gggtaacgcc tttgtcaccg
     5641 aggcaaaacg tcaggtgagc cagcgtctgg acggtgcggc gatcgatatg cccgcaggcc
     5701 cgtcggaagt gttggtcatt gccgacagcg gcgcaacgcc ggatttcgtg gcttctgatt
     5761 tgctttctca ggctgaacac ggcccggact cacaggtgat tttactgacg cctgacgctg
     5821 atatggcgca tcaagttgcc gaagccgtcg aacgccagtt agcagaactg ccgcgtgccg
     5881 aaaccgcccg ccaggcactg aacgccagcc gcctgatcgt gactaaagat ttagcgcagt
     5941 gcgtggagat ctccaaccag tacggcccgg agcacctgat cattcagacc cgcaacgccc
     6001 gcgaactggt cgatggcatc accagcgccg gttcggtatt tcttggtgac tggtcaccgg
     6061 aatcggcagg tgattacgcc tccggaacca accatgttct accgacttac ggctacaccg
     6121 ccacctgttc cagcctcgga ctggcggatt tccagaagcg gatgaccgtg caggaactgt
     6181 cgaaagtagg tttctccgcg ctggcttcga ccattgaaac actggccgcc gccgagcgcc
     6241 tgaccgccca caaaaatgcc gttactttgc gtgttaacgc ccttaaggag caagcatgag
     6301 caccgtgact attaccgatt tagcgcgtga aaacgtccgc aacctgacgc cgtatcagtc
     6361 ggcgcgtcgt ctgggcggta atggcgacgt ctggctgaac gccaacgaat atcccacagc
     6421 cgtggagttt cagcttactc agcaaacgct caaccgctac ccggaatgcc agccgaaagc
     6481 ggtgattgaa aattacgcgc aatatgcagg cgtaaaaccg gaacaggtgc tggtcagccg
     6541 tggcgcggac gaaggtattg aactactgat tcgcgctttt tgcgaaccgg gtaaagacgc
     6601 catcctctac tgcccgccaa cgtacggcat gtacagcgtc agcgctgaaa ccattggcgt
     6661 cgagtgccgc acagtgccga cgctggaaaa ctggcaactg gacttacagg gcatttccga
     6721 caagctggac ggcgtaaaag tggtctatgt ttgcagcccc aacaacccaa ccgggcaact
     6781 gatcaatccg caggattttc gcaccctgct ggagttaacg cgcggtaagg cgattgtggt
     6841 tgccgatgaa gcctatatcg agttttgccc gcaggcatcg ctggctggct ggctggcgga
     6901 atatccgcac ctggctattt tgcgcacact gtcgaaagct tttgctctgg cgggccttcg
     6961 ttgcggattt acgctggcaa acgaagaagt catcaacctg ctgatgaaag tgattgcccc
     7021 ctacccgctc tcgacgccgg ttgccgacat tgcggcccag gcgttaagcc cgcagggaat
     7081 tatcgccatg cgcgaacggg tggcgcaaat tattgcagaa cgcgaatacc tgattgccgc
     7141 actgaaagag atcccctgcg tggagcaggt ttttgactct gaaaccaact acattctggc
     7201 gcgctttaaa gcctccagtg cagtgtttaa atctttgtgg gatcagggca ttatcttacg
     7261 tgatcagaat aaacaaccct ctttaagcgg ctgcctgcga attaccgtcg gaacccgtga
     7321 agaaagccag cgcgtcattg acgccttacg tgcggagcaa gtttgatgag tcagaagtat
     7381 ctttttatcg atcgcgatgg aaccctgatt agcgaaccgc cgagtgattt tcaggtggat
     7441 cgttttgaca aactcgcctt tgaaccgggc gtgatcccgc aactgctgaa gctgcaaaaa
     7501 gcgggctaca aactggtgat gatcactaat caggatggtc tgggaacaca aagtttcccg
     7561 caggcggatt tcgatggccc gcacaacctg atgatgcaga tattcacctc gcaaggcgta
     7621 cagtttgatg aagtgctgat ttgtccgcac ctgcccgccg atgagtgcga ctgccgtaaa
     7681 ccgaaagtaa aactggtgga gcgttatctc gctgagcaag cgatggatcg cgccaacagt
     7741 tatgtgattg gcgatcgcgc gaccgatatt caactcgctg aaaacatggg cattaatggt
     7801 ttacgctacg accgcgaaac cctgaactgg ccaatgattg gcgagcaact caccagacgt
     7861 gaccgttacg ctcacgtagt gcgtaatacc aaagagacgc agattgacgt tcaggtgtgg
     7921 ctggatcgtg aaggtggcag caagattaat accggcgttg gcttctttga tcacatgctg
     7981 gatcagatcg ccacccacgg cggtttccgt atggaaatca acgtcaaagg cgacctctat
     8041 atcgacgatc accacaccgt cgaagatacc ggcctggcgc tgggcgaagc gttaaaaatt
     8101 gccctcggcg ataaacgcgg tatttgccgc tttggttttg tgctgccgat ggacgaatgc
     8161 cttgcccgct gcgcgctgga tatctctggt cgcccgcacc tggaatataa agccgagttt
     8221 acctaccaac gcgtgggcga tctcagcacc gagatgatcg agcacttctt ccgttcgctc
     8281 tcttacacca tgggcgtgac gctccacctg aaaaccaaag gtaaaaacga tcatcaccgt
     8341 gtagagagcc tgttcaaagc ctttggtcgg accctgcgcc aggccatccg cgtggaaggc
     8401 gataccctgc cctcgtcgaa aggagtgctg taatgaacgt ggtgatcctt gatactggct
     8461 gcgccaacct gaactcggtg aagtctgcca ttgcgcgtca cggttatgaa cccaaagtca
     8521 gccgtgaccc ggacgtggtg ttgctggccg ataaactatt tttacccggc gttggcactg
     8581 cacaagcagc gatggatcag gtgcgtgagc gcgagctgtt tgatctcatc aaagcctgta
     8641 cccaaccggt gctgggcatc tgcttaggga tgcaactgct ggggcggcgc agtgaagaga
     8701 gcaacggcgt cgatttgctg ggcatcatcg acgaagacgt gccgaaaatg accgactttg
     8761 gtctgccgct gccgcatatg ggctggaatc gcgtttaccc gcaggcaggc aaccgcctgt
     8821 ttcaggggat tgaagacggc gcgtactttt actttgttca cagctacgca atgccagtca
     8881 atccgtggac catcgcccag tgtaattacg gcgaaccatt caccgcggcg gtacaaaaag
     8941 ataacttcta cggcgtgcag ttccatccgg agcgttctgg tgccgctggc gctaagttgc
     9001 tgaaaaactt cctggagatg tgatgattat tccggcatta gatttaatcg acggcactgt
     9061 ggtgcgtctc catcagggcg attacggcaa acaacgcgat tacggtaacg acccgctgcc
     9121 gcgcttacag gattacgccg cacaaggtgc cgaagtgctg catttggtgg atctgaccgg
     9181 ggcaaaagat ccggcaaaac gtcaaatccc gctgattaaa accctggtcg ctggcgttaa
     9241 cgttccggtg caggttggcg gcggcgtgcg tagcgaagaa gacgtggcgg cgttactgga
     9301 agctggggtt gcgcgcgtgg tggttggctc cactgcggtg aaatcaccag aaatggtgaa
     9361 aggctggttt gaacgtttcg gtgccgatgc cttagtgctg gcgctggatg tccgtattga
     9421 cgagcaaggc aacaagcagg tggcggtcag cggctggcaa gagaattcgg gcgtgtcact
     9481 ggaacaactg gtggaaacct atctgcccgt cggcctgaaa catgtgctgt gtaccgatat
     9541 ctcgcgcgac ggcacgctgg caggctctaa cgtctcttta tatgaagaag tgtgcgccag
     9601 atatccgcag gtggcatttc agtcatccgg cggcattggc gacattaacg atgttgctgc
     9661 cctgcgtggc actggcgtgc gcggcgtaat agttggtcgt gcattactgg aaggtaaatt
     9721 caccgtgaag gaggccatct catgctggca aaacgcataa ttccatgtct cgacgttcgt
     9781 gatggtcagg tggtaaaagg cgtacagttt cgcaaccatg aaatcattgg cgatatcgtg
     9841 ccgctggcaa aacgctacgc cgaagaaggt gcagacgaac tggtgttcta cgatatcacc
     9901 gcttccagcg atggccgtgt ggtagataaa agttgggtat ctcgcgtggc ggaagtgatc
     9961 gacattccgt tctgcgtggc gggtgggatt aagtctctgg aagatgccgc gaaaattctt
    10021 tcctttggcg cggataaaat ttccatcaac tcccctgcgc tggcggaccc gacgttaatt
    10081 actcgcctgg cggatcgatt tggcgtgcag tgtattgtgg tgggtattga tacctggtac
    10141 gacgctgaaa ccggtaaata tcatgtgaat caatataccg gcgatgaaag ccgcacccgc
    10201 gtcactcagt gggaaacgct cgactgggta caggaagtgc aaaaacgcgg tgccggagaa
    10261 atcgtcctca acatgatgaa tcaggacggc gtgcgtaacg gttacgacct cgaacaactg
    10321 aaaaaagtgc gtgaagtttg ccacgtcccg ctgattgctt ccggtggcgc gggcaccatg
    10381 gaacacttcc tcgaagcctt ccgcgatgcc gacgttgacg gcgcgctggc agcttccgta
    10441 ttccacaaac aaataatcaa tattggtgaa ttaaaagcgt acctggcaac acagggcgtg
    10501 gagatcagga tatgttaaca gaacaacaac gtcgcgaact ggactgggaa aaaaccgacg
    10561 gactgatgcc ggtgattgtg caacacgcgg tatccggcga agtgttaatg ctgggctata
    10621 tgaacccgga agccttagac aaaaccatcg aaagcggcaa agtcaccttc ttctcgcgca
    10681 ctaaacagcg actgtggacc aaaggcgaaa cgtcgggcaa tttcctcaac gtagtaaata
    10741 ttgcgccgga ctgcgacaac gacacgctac tggtactggc gaatcctatc ggaccaacct
    10801 gccacaaagg caccagcagc tgcttcggcg acaccgctca ccagtggctg ttcctgtatc
    10861 aactggaaca attgctcgcc gaacgcaaat ctgccgaccc ggaaacctcc tacaccgcca
    10921 aactgtatgc cagcggcacc aaacgcattg cgcagaaagt gggcgaagaa ggcgtggaaa
    10981 ccgcactggc cgcgactgta catgaccgct ttgagctgac caacgaggcg tctgacttga
    11041 tgtatcacct gctggtattg ttgcaggatc aggatctgga tttaacaacg gtgattgaga
    11101 acctgcgtaa acgtcatcag tgagttgcgg ggtaagcgga tgcgatattg ttgtcgcatc
    11161 cggcaaaaaa aacgggcaag gtgtcaccac cctgcccttt ttctttaaaa ccgaaaagat
    11221 tacttcgcgt tgtaattacg cagagcgtta cgccccagca caatccccgc gccaaccatg
    11281 ccgcccagca gcaccgccag aatcaaggtg attgcctttt tcggactatc gcgacgaata
    11341 ggtaacgtcg gtttcattac atagcggtaa gcatgaatat caagatcatc aacctttaag
    11401 ttttcaatat ccaacaggtt ttgacgtgtc tgatagtagt ttggtgagaa caccaacgga
    11461 cgggtcgcct catgcttaat catcgactcc agcgcttcgc tccccaaaag gaacaacgta
    11521 tcttgcgtca catcttcagt ctgctgaacc tgtggctttg ccacctgcgc ctgattcgca
    11581 tactgcaacg cttcctgaat ctgacggata cgcagctctt tctgctcctg cgcaactact
    11641 tcctgcgttc ttaaagagtc ctgcaagttt ttccgtccca gagcaatgtt gtctttgaga
    11701 tccttttcta gctcttgatt cactttatca tcaacttgct gaatgtattg ggccaacttc
    11761 atttgtgctc cctctgcagt ttgcccaaca taagaaacag tcaatggtaa ttgctggttc
    11821 ttaacagaag gttcgatggt aagtttttct ggttcttcct gattatccag cgtttctgct
    11881 aatgctgaga aggcagaact gaagcgacca atcaacgtct cctgcaaatc cgatactttc
    11941 ggtgcagcct gaccatagat aacattcatg gcattgttat agccagcaat ttgccccacg
    12001 tcgggctgag tgataattgc tgttgacgtc catttctcct tcgctactgc caaataacca
    12061 atagccagag caatagccac aatgacggaa atgatgattg tcatcttgcc acgccacaac
    12121 tgcactagta aatcaatcaa atcaatctgt tccgggtcat ggttttgccc agaaacatta
    12181 ttattttcta ctctcatcat taccctaact gacggaaatt tccaagtccg catagtttag
    12241 cgataatccc tgtgaatgct ataggataag tgataaaaat aatgaattat tgcggaggta
    12301 acaaaagaca atcagggcgt aaatagccct gataacaaga tgttagtcgc tgccaaagag
    12361 atcgcgggtg tagaccttat ctgccacatc cctaagctct tctgccatac ggttggaaat
    12421 aatgacgtcg gcttgttgtt tgaaggtggc gagatcacgt tccaggcgag agttgaagaa
    12481 tgagtcttct ttcatcactg gctcgtagat gattacttca acacctttcg ccttgatacg
    12541 cttcataatc ccctgaatag aagacgcacg gaagttatct gaaccgctct tcataatcag
    12601 acgataaata cctaccactt gcggcttgcg tgacaaaatg gcatcggcaa taaaatcttt
    12661 acgcgtgcgg ttagcatcga caattgccga gatcaggtta ttcggcacag actggtagtt
    12721 cgccagtaac tgcttggtat ctttcggcaa acaataacca ccataaccaa acgacggatt
    12781 gttgtaatgg ttgccaatac gtgggtcgag acaaacgcct tcgattattt gacgggaatt
    12841 cagacctaaa ctttctgcgt agctatccag ttcgttaaag tacgccacgc gcatcgcgag
    12901 gtaggtgttt gcaaaaagtt taatcgcttc tgcttcagtg gagtcggtaa acagggttgg
    12961 gatattttgc ttaatcgcgc cttcctgtaa taacgcagca aaacgttctg cgcgttccga
    13021 acgctcaccg attacaatac gtgacggatg aaggttatcg taaagagctt taccttcgcg
    13081 gagaaattcc ggagagaaaa taatattttc agtacgatat ttcttatgca tcgcttcggt
    13141 aaaaccaacg ggaaccgttg atttgatgac cataaccgca taaggattta tctcaactac
    13201 gtctttaatt actgattcta cactggatgt attgaaataa ttagttttag gatcatagtc
    13261 ggttggagtg gcgatgatga cataatcagc atcccggtag gcttcatttt tatctaatgt
    13321 ggcattaaag tgtattttat ctgattgcaa aaactgctga atttccttat caacaatagg
    13381 agatatccga tcattcagca tagcaacgcg tgacggtaaa atatctaatg ccacaacctc
    13441 atgattttga gcgattagaa gcccgtttga caagcctaca tagccagtac cggaaatggt
    13501 gattttcatt tcatgctctc agaattaact taactgtgaa tcatgatgtt tttaacatcc
    13561 tgataagagc taaaagtttt aacgctacgt tgttcgatgg tcaacgcagc gaaaaacaat
    13621 tcagattttc tttataagaa tagctgatat ttattaagtt aatattaagc agtgatattt
    13681 agtctataag ataattggca aaaaaaagcc cggtacaata tacgccgggc ctcaatttta
    13741 ttgttgatta aatcagatta atccagccat tcggtatgga acacaccttc tttatcaatg
    13801 cgcttataag tatgcgcacc gaaatagtca cgctgtgcct ggatcaggtt cgcaggcaga
    13861 acagcggcgc ggtagctgtc ataataggca accgcagcgg cgaaggtcgg aaccgggata
    13921 ccgttctgta ccgcataagc gacgacatcg cgcagcgcct gctggtagtc atcggcaatt
    13981 tgcttgaagt aaggagccag cagcaggtta gcgatctgcg gattttcggc ataagcatcg
    14041 gtgattttct gcaggaactg cgcacggatg atgcagccag cacggaaaat cttcgcgatt
    14101 tcgccgtagt tcagatccca gttgtactct tcagacgccg cacgcagttg agagaacccc
    14161 tgagcgtaag aaacgatttt gcccagatac agtgcacggc gaactttttc gatgaactca
    14221 gccttgtcgc cagctggctg cgcttgtggg ccagagagaa ctttagacgc agcaacgcgc
    14281 tgatctttca gagaagagat gtatcgtgca aacacagact cggtaatcag cgacagcggt
    14341 tcgccgagat ccagtgcgct ctggctggtc catttgcccg tacctttgtt tgccgcttca
    14401 tccaggatca catcaaccag gtagttaccg tcttcatctt ttttagtgaa gatgtctttg
    14461 gtaatgtcga tcaggtagct gctcagttca ccgttattcc actcggtaaa gatctgcgcc
    14521 agttcttcgt tggtgaggtt cagaccacct ttaagcagag aataggcttc agcaatcagc
    14581 tgcatatcgc cgtattcaat accgttgtga accatcttca catagtgacc tgcgccatcg
    14641 gcaccaatat aggtaacgca tggctcaccg tcttcagcca ctgcggcgat tttggtcagg
    14701 atcggcgcaa ctagttcata ggcttctttc tgcccaccag gcataatgga aggacctttt
    14761 agtgcgccct cctcaccacc ggaaacaccg gtaccgatga agttaaagcc ttctgcagaa
    14821 agctcacggt tacgacgaat ggtgtcctgg aagaaggtat taccaccatc aatgatgatg
    14881 tcacctttat cgaggtatgg cttaagggaa tcaatagcag catccgtgcc tgcacctgct
    14941 ttcaccatta acaagatgcg acgaggcgtt tccagagatt caacaaattc tttcaccgta
    15001 tagtaaggaa ccagtttctt gcctggattt tcggcaatca cttcttccgt cttttcacgg
    15061 gaacggttga aaatagagac ggtataacca cggctttcga tgttgagcgc aaggttgcgc
    15121 cccatcactg ccataccgac tacgccgatc tgttgctttg acattgttta ctcctgtcag
    15181 gggttaccgc tagatatgcg gcttgaataa cgtataaaaa tgtatcagag tattataaat
    15241 ttcatgctgt atcagtaata actaagtaaa ggatagctta aaaagaagcc ataatttagt
    15301 aaattagcga aattatatat attttaaaca ttagtcccat ttggaacgtg ttttcgcatt
    15361 cccattaaat agatgttggc gatcttttca tttctcttgc tggatttccg cacacagtta
    15421 taccggcagg aacactttta gtgacaacag cccccatacc tataatggct cccgcgccaa
    15481 taataagtgg gcgattagga acaccctgct taattactgc acccgagcct atatatgcat
    15541 tgtcttcaat aacaacatat ccattacatt tagccccagg agcaaatgta acatagtctc
    15601 ctatttgaca atcatgtgca acgtatgagt atatgtttgc atgaaaaaac ctccctatat
    15661 gagtattagt agatattgta acaaagggag aaataatagc gccactacct atcatagtat
    15721 gatcataaac aacgctattt ggatgtttta tagttattgg ttcaaccccg tgtaataata
    15781 ttgactcaga cactctctgt cgtatcttat cattagcaat agcaacatta aaatactttt
    15841 ttaaataagg ggcttttaga aagcagttgg ttgaaagcac ttcaaaacca ttaactttct
    15901 ttcctgccaa aacatcatcc acaaaaacca gagcatagtc agaaccacat tcttgcttta
    15961 tttgttgatt tagaatgggt attgtttctc taccataact tccagcacca aaaataccat
    16021 acaaattcat tttcccatct ccaaagaaat atcttatcaa aaccaatgca agattatgaa
    16081 agcccgcacg ctgcaatgcc tgacgctagg aagtttctgt ccatgactgc ttttcgcatc
    16141 ttacatgata atcctgcctt gaatacatta tcattgtcag atattaatag cgatgtgccg
    16201 cattctagaa aatcattcga atgcaactcg ccttcttata ttatagtcga tttcattcat
    16261 taccacatca ggctactgtg caacttattc tccatgcgcc agtgatttcg atttactgtg
    16321 gcgaacttct ctgcggttaa agcagcagat ctaatataat atttgatcgt catttcggat
    16381 tctttctttt gctctgctat tattgagaga aagtggactg ccattcataa attctgcagc
    16441 cctttccatt caaacgtgaa atcaataagc tcatcaggag catctcaaac aatatgaaga
    16501 cggacatcgt ctctgccgtg cctcttttca ctaattgcgt aactgtcatg ttttggatta
    16561 tttaattctt tcagcgtaaa tatctcctca aagactctat taagccgact cttatttcct
    16621 tttacagcga ataaataatc acatctctgt ttttatatct tctctgcaat atctttctgg
    16681 caagccatcg catcagttat gattattttt cctttaatac ccatcatttt agaaagttca
    16741 tgaataactg tgaacgcatt aggtttctca tccgtcttga tctatccgag gaccagactg
    16801 tgcattgctg aaacgcacta atgacatgaa ccactcctct gcgacgactc ttgtcataag
    16861 tgtgccgaag tgttttacca tcaattgcga tgacgtcgtt atcatctgga aaatggcaga
    16921 catgtattca gttaacaaag cactcgtgaa atattgcagg actgatgcag gatacaactt
    16981 tggctataat atcgtaaaca gggataataa aaatctattg tctcaattat tttaagttcc
    17041 attttgttct cctttttata taaggcacat ttgttattac ataagcgatg aaaaaatgtc
    17101 ttaagcaaag gaacgtctca atcgtgacgt gaaatgcaaa ttggtcagta caaaacttta
    17161 tttctattaa tcttttccta gataaagttg cggtatagtc gttatacata aaaaagccca
    17221 catattcatc atataccgca ataacattac tttaatataa tcttaaatac gcttaatgat
    17281 taatgtaaat aatcactcac ttagcaattt aagaagagct ttagttttct tttccattag
    17341 ctttacatca ccgcgtgatt ccacattcaa ccgcaccacc ggttcggtgt tggaggagcg
    17401 caggttaaag cgccagtcgg caaaggtcat gctgatgcca tcggtgcgat ccaccgccag
    17461 cgcctcgcgg ctaaaatgct gttccacgcg attaattgcc tcaacgggtt gcgccagttt
    17521 gctgttgatc tcaccgcttg ccggaaacgc cgccatccgg tcgcgcacca tttcgcccag
    17581 cgtttttcct ttcaggcaca ccagttcggc gaccagcagc cacgggatca tgccgctgtc
    17641 gcagtaagcg aaatcacgga agtaatggtg agcgctcatt tcgccaccgt agatggcgtc
    17701 ttccttgcgc atacgttctt taataaaggc gtgtccggtt ttcgacatta ccggggtgcc
    17761 gcctgcggca gtcaccacat caacggtgtt ccaggagaga cgtggatcgt ggatgatctt
    17821 cgcgccggga tttttttcga ggaacgcttc tgccagcagg ccgacaatgt agtagccctc
    17881 gataaactgc cctttttcgt caaacaggaa acagcggtca aaatcgccat caaaggcaat
    17941 gcccatatcc gcgccgtgtt tgatgaccgc attacgggtg tcgtcgcggc attccggcag
    18001 cagcgggtta ggaataccgt tggggaaatt gccgtccggc gtgttgtgta ctttgattaa
    18061 ttccaccggt gcgccgaggg ctttaaatcg ggcttcaatg gcgtccacca ccggacccgc
    18121 tgcgccgttc ccggagttga tcaccagctt gagcggcgtg aggtttttga cgttgatata
    18181 accgaacagg tgatcaacgt aagcgtcacg cagattgatt tgctgatagc gaccacgttt
    18241 ggtttcatcg acaggaggga agtcattggc ttctgccaga cgctggacat cgcgcagtcc
    18301 ggtatcaccg ctgatcgggc gagccccttc gcgcaccagc ttcatgccgt tgtaatccat
    18361 cgggttatgg ctggcggtaa cttcgatgcc gccatccact ccgagatgga acgtggcgaa
    18421 atagatctct tcggtgccgg acataccgat atccagcaca tcgacgcccg catcctgtaa
    18481 acctttcgca agcgccagtt ttaacgcttc gctggtgagg cggacatcac cgcctaaaac
    18541 aatggttttc ggtttgagaa attcgccata ggcacgccca atgcgccagg caatatcttc
    18601 attcagttct tcgcctaatt tcccgcgaat atcataggct ttaaagcagg ttaaagattt
    18661 catatgttaa tcttcatgtt tgtaacgttc tttctgtctt ataatatcat cctctcccaa
    18721 ataatcccct gaactgactt caataagatt aagagggatt atgcccggat tctcaagact
    18781 atacgctgcg ccaaggggaa tgtatatcga ttcatttgcg gtgactagtt tagttttatc
    18841 gccaagggtt acttttgctg taccagaaag cacgatccaa tgttcagaac gatggtgatg
    18901 catccttaaa gaaagcccct caccaggttt cacaataatt ttcttgactt tgtatcgctc
    18961 accttggtca atcgaatcaa attttcccca tggtcggaaa acttcacgat gactaatata
    19021 ctctgtacgt tgctgcaatt taagcatttc gactattttt tttacatgct gtacatcact
    19081 ctttttagac acaagaacgg catctttagt ttgcacgata accatatctt caattccaat
    19141 ggcggctacc aacgctgact cagagtagat ataattattc ttagtatcat aggttaatat
    19201 atcaccttta catacatctc ctgttttcga ttttagacta atgtcccata acgattgcca
    19261 agatccaacg tcactccaac caatatcaac agggcataca acacattttt ctgttttttc
    19321 cattacagca aaatcaatag attcagcagg acaatcttga aattgttctt ttgataatcg
    19381 aataaaatct agatcaatgt atgaggatga ggcaacctgt tcacaaacat tgtaaatgtc
    19441 aggtctaaat tttctcaatt cctcaagata aacagatgcc ttaaacatga atattccact
    19501 attccaatag tgattaccag aagtcatata caattctgcg gtttcacgat taggcttttc
    19561 gacaaactta tttacataat aaaaaccagt attttcatgc ccttgaagcg gtacagatag
    19621 ttcacctctc tcaatatacc cataaccagt ttcagcatat tctggtataa ttccaaacgt
    19681 tacaatttta ccttgattag cgatgggagt tgcattttta atagcatcac agaaaacact
    19741 ttctttagct ataacgtggt ctgccgcaag aactagaagc aatggatctt cctgaggatt
    19801 acgttttaac gcatgaaacg cagatatcgc tattgctggt gcagtatttc gcccgcatgg
    19861 ttctagaata atattaccat ttaatttatt tatttccctt aactgttcag ccacaacaaa
    19921 gcgatgctgt tcatttgtta tcactaatgg tttttgacat gataggcctg aaagtcgtag
    19981 caaagtcgtt tgtaacaagg tgttatcacc agagagttgt aaaaactgct ttggatatag
    20041 ttcacgagaa agtggccaaa gacgactgcc tgtaccaccg gccataacta cagcaattat
    20101 tggcgcatca gacatattat atccttatta tctaagccaa tcgcatcatt tgttttattg
    20161 ttaaaatatg cgcgacaatt ataatgcaca tcgtcatcat ttatcagctt tgctcgcgat
    20221 agccagcaat aagcattatg ttgtgacttc ggtaatttca aaatgtttgg ttgtaattta
    20281 agaaggaatg ctataactat ataatgcgtt gaaaagtctc cccccatact attgtcttcg
    20341 tagaagtgct gccagatacc ataaaactta cccacagaga gaggcaaacg aattcctagt
    20401 tcaatttctg tcaatcgttc aaaggctgtc tgcaattttt catctttcaa caccctacca
    20461 ccaggaacga accaatagcc ctgtgccggg cggttgattc gttttcctag caaaatttcg
    20521 ccaaactcgt tttccacaat caaatctata gaaataagag gagtagacct tacaattgtg
    20581 gcaaagtctt gggaatgtaa aaacattatt acccccgata ttgaagttgg ttttcaagaa
    20641 accagttgta tgtattttca agacccttgt gaagggtaat tttatgattc caacctagtt
    20701 gatgaagaag cgttacatca agtagttttc gaggggctcc atcgggcttt gttgtatcga
    20761 acgtaatatg ccctttataa cctacaactt ttgctattgt ttccgcaagc tcacaaatcg
    20821 tgcagtcaat acctgttcca atattgatat gagacaacat tactttagta tttttttgcc
    20881 atatatcgta tggcatctcc atgacataaa tgcttgcaga agccatatca tctacatgta
    20941 agaattcacg ctttggagta ccacttcccc aaacaacaac attcggagaa ttgttttcca
    21001 cagcatcatg aaagcggcgc aaaagcgccg gaatcacatg agaattactt ggatgaaaat
    21061 tgtcatttgg accataaaga ttggttggca ttactgaacg gtaatcacgc ccaaactgac
    21121 ggttataaga ttcacataat ttaatacctg caatttttgc gatagcataa ggttcatttg
    21181 ttggctcaag tttcccttgt aataattcgt cttccataat cggttggtgt gctaacttag
    21241 gataaataca cgacgaaccg aggaaaagca gtttatttac attatttttg tgggcagcat
    21301 gaatgacatt cgcctctatc attatattct catatataaa atcggcagga taagaactgt
    21361 tagctaaaat acctccgact tttgctgctg ccaaataaac ctggtcgatt ttctgtgaag
    21421 aaaaaaaatc caaaacagcg ctactatcca acaagttcaa ttcatcccga gtacgtaaaa
    21481 ccaactcaac atcatcacgt tgtttgaggc gtcgggtaat agctgatcca accattcctt
    21541 ggtgaccagc aataaaaata cgttgcttat tcatcatcat tccagagcta agcttacaga
    21601 aaaaccatgc gattttaaaa gagaatgttt tttagcggct tcaagatctt tggcaaccat
    21661 ttcagaaatc atttcagcaa gagtaatttc tggtctccaa ccaagtttga gattagcttt
    21721 gctcggatct ccaagcaaag tatcaacttc agctggtcgg aaataacgag gatcaacagc
    21781 aacaatgaca tcacctggtt tcacacctgg agcatcctgt ccttcaaccg aatctacaat
    21841 gcctttttct tcgattcctt taccaacaaa gctcatctta ataccaagtt gtgctgctgc
    21901 catttcgaca aactgacgga ctgagtattg gactcctgtt gcaatcacaa aatcttcggg
    21961 ttgctcctgt tgtaacatca accattgcat tctaacataa tcttttgcat gtccccaatc
    22021 tcgtaacgaa tccatattcc ctaaatacaa acatgattcc aagccttgtg caatatttgc
    22081 aagtcctcga gtaattttcc ttgttacaaa cgtttctcca cggcgtggag attcatgatt
    22141 gaacaatata ccattacatg cataaatacc atatgactct cgataattta ccgtgatcca
    22201 atatgcgtaa agttttgcaa ctgcataagg ggaacgagga taaaaagggg tggattcttt
    22261 ttgagggatt tcctgaacaa gtccatataa ttctgaggtt gaagcttgat agaaacgcgt
    22321 tttgttttcc aatcctaaaa agcgaattgc ttccagtaaa cgtaatgtac caattgcatc
    22381 gacatcggct gtatattctg gagactcaaa agaaactgct acgtgactca tagcagctaa
    22441 attatatact tcatctggct gtacctcctt tagaattcta gtgaggttag atgaatcagt
    22501 cagatctcca tagtgcaagt gaaaatttgg gttagaacca tgtggatctt gataaatatg
    22561 gtctatgcgt tctgtattaa aagatgaggc tcggcgtttg ataccatgaa cttcataccc
    22621 tttatcaagc aaaaactcag ctagataaga tccatcttgt ccagttacac ctgtaataag
    22681 agcgacttta gtcattatat acctcttgaa tatattaaga tcgggattca atttcttgtt
    22741 tcacaacatc taaaagaaaa tctgcacacg cattgcgtct tttgagaccc aattctatca
    22801 gagttgtagg ttcataaaaa attttagatt catcagcttt taccatcgcg tcatttaatg
    22861 aataatggtt ttttgcctga aagaaatata catcaccgca attaacttct ttattgacat
    22921 ctatgtcaga tagtataact tttttcccta atgcaatagc gtcaaatgtt accccccctc
    22981 caggcccgcc ttcaaataag gttggttgta ttacagcaat gcaatttttg attaattcaa
    23041 tttgttcaag tttaggtata tgccctaata tcttaatttt cgattcaatt ccgagctttt
    23101 ttgccaaaac catcaattca ttaaaatatc cagggaatcg ataatcttga gtagctcccg
    23161 tgcatactaa ataaacatca ggattatatt cagtataaat tttaaatgcc ctaaaagcag
    23221 ttgcatgatc tttatgtttc caaaattgat tgcaaattat
//
LOCUS       DAZFAB010000075        22011 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZFAB010000075 DAZFAB010000000
VERSION     DAZFAB010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22011
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.075"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            623..1789
                     /gene="sopA"
                     /locus_tag="ACHJWA_003225"
     CDS             623..1789
                     /gene="sopA"
                     /locus_tag="ACHJWA_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid-partitioning protein SopA"
                     /protein_id="HFO4309770.1"
                     /translation="MKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRIGE
                     AADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRAE
                     DVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPT
                     DPHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQ
                     FFDMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRE
                     TDEVGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     gene            1789..2760
                     /locus_tag="ACHJWA_003226"
     CDS             1789..2760
                     /locus_tag="ACHJWA_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family plasmid partition
                     protein"
                     /protein_id="HFO4309771.1"
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGN
                     AITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQ
                     TPAFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSA
                     YERGQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSA
                     RSGDALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLS
                     SRHQFAPGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     gene            3455..4357
                     /locus_tag="ACHJWA_003227"
     CDS             3455..4357
                     /locus_tag="ACHJWA_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFO4309772.1"
                     /translation="MYFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QTTEDVRFEPCPGLTAAGRGVVSPENIAFTRWLTHLQDGVLLDEQNCLMLHELWLQSG
                     TGRRRWEELPDDARESITALFTPKRGDWCDIWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLSTRLDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPCGYEVNISSQGDNFIQVDF
                     DTPWCQPESDVIAVLSRRFSCMLEHWYAGQGCNFCGWQRYERGELVDVLWGELEWSSP
                     TDDDELPEVTAPEWIVDKVAHYGG"
     gene            4361..4666
                     /locus_tag="ACHJWA_003228"
     CDS             4361..4666
                     /locus_tag="ACHJWA_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4309773.1"
                     /translation="MTETGGQPPVSFPVKDVAGLLFLLRRLTRRGRNAACGQCPASGR
                     ARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGIFSPSKRRRLRYSTVGLTRYRTR"
     gene            4743..5426
                     /locus_tag="ACHJWA_003229"
     CDS             4743..5426
                     /locus_tag="ACHJWA_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFO4309774.1"
                     /translation="MSRFIQGDCVRVMATIPGNGVDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKTYT
                     SKAAYVGYRHECAYILAKGRPALPQKPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            5427..5648
                     /locus_tag="ACHJWA_003230"
     CDS             5427..5648
                     /locus_tag="ACHJWA_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4309775.1"
                     /translation="MNYAGHEKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LARQTLYRANRLFMEAYTEILELDACFKD"
     gene            5542..6096
                     /locus_tag="ACHJWA_003231"
     CDS             5542..6096
                     /locus_tag="ACHJWA_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO4309776.1"
                     /translation="MCLLNAWHARRCIAPIACSWRRIPKFWNWMRALKIEKETRMYGT
                     CETLCRELAAKYPGYTPLMLVIWSPEEIQALADGMDISLSDPEIRTVLARLEDIPEDQ
                     RIESGISSAAAMEIISNVSENRQVTVPAELLASLIQTAEQALWKREWAARDYGLAVPE
                     CVTRRQAVVNQARILLKNNTHENG"
     gene            6142..6918
                     /locus_tag="ACHJWA_003232"
     CDS             6142..6918
                     /locus_tag="ACHJWA_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4309777.1"
                     /translation="MNENTTLNALICRHARNLLLAQGWPEETDVDQRNPKYPGWISIY
                     VLLDAPRLATLLVNRHGGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQV
                     SFPYAGEWLTEDEIRAVLAAVRDAVRSVSCRVAEDTRRIRAALTTTGQTLLTRQTRRF
                     RLVVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDV
                     LRIPDEPPRRWFDRGVLREVVREARNEIRSMADALAKIRK"
     gene            7336..7761
                     /locus_tag="ACHJWA_003233"
     CDS             7336..7761
                     /locus_tag="ACHJWA_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO4309778.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADACGIILTSLVINRQLW
                     LYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            7808..8230
                     /locus_tag="ACHJWA_003234"
     CDS             7808..8230
                     /locus_tag="ACHJWA_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO4309779.1"
                     /translation="MYCTVKEIIRDVLDTDVPDSECVFAVVLTRGDVRHIAQDWNLSD
                     DELETVMQRLDDAFEYGADVSIVHDVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            8227..8418
                     /locus_tag="ACHJWA_003235"
     CDS             8227..8418
                     /locus_tag="ACHJWA_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4309780.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            complement(8388..8897)
                     /locus_tag="ACHJWA_003236"
     CDS             complement(8388..8897)
                     /locus_tag="ACHJWA_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001358895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4309781.1"
                     /translation="MSRDPPQRREARFAKRRCPLHPGGLLPGCRERRPRRDRGCATGT
                     VAASQGFASGMEARQGGDSFAGSVHDSPPRQGDARKSHKQERNGKKRRKRKRKKKKNS
                     NRHGNHRKITSEKNIPDTSQTGRVTDDASAWPASLPAGVSLPGVFARADRRPPPPALY
                     HLFLLPCKM"
     gene            9414..9644
                     /locus_tag="ACHJWA_003237"
     CDS             9414..9644
                     /locus_tag="ACHJWA_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4309782.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPAPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            9696..11057
                     /locus_tag="ACHJWA_003238"
     CDS             9696..11057
                     /locus_tag="ACHJWA_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HFO4309783.1"
                     /translation="MTSAAITMTAPEAASPVQMYRATYSPDDNKLRLYAVSRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERGRSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLNYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVAEAEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            11104..11667
                     /locus_tag="ACHJWA_003239"
     CDS             11104..11667
                     /locus_tag="ACHJWA_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFO4309784.1"
                     /translation="MHSQLKERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVT
                     LAEISNRDHILEPSAGTGAILRAIRDTAPEAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWQPVQYYSRVIINPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREELPRGTFAYTDVPTMIIRLRA"
     gene            complement(11753..12214)
                     /locus_tag="ACHJWA_003240"
     CDS             complement(11753..12214)
                     /locus_tag="ACHJWA_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4309785.1"
                     /translation="MAPDTTTGRHPPQRREARFAKRRCPLHPGGLLPGCRERRPRRDR
                     GGATGTVAASPGFASGMEARQGGDSSAGSVHDSPPRQGDARKRHKQENNKTDSNQSSR
                     KKKNSSSSSSSSSSSSSSSSMNNNIISNRQMTGLKRQKPTAECPRNVRGGA"
     gene            12284..12490
                     /locus_tag="ACHJWA_003241"
     CDS             12284..12490
                     /locus_tag="ACHJWA_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO4309786.1"
                     /translation="MHIPQNFLPKSRQKVLRSAPGQSFPAVAVMPLAAKVLKKVMVNT
                     GGNKRQVPAKRQNRGSRNSKDDGG"
     gene            12516..12968
                     /locus_tag="ACHJWA_003242"
     CDS             12516..12968
                     /locus_tag="ACHJWA_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO4309787.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLVRAAGAQTQPEEGQQFSGQPQPQPPEGDDYGFSDDIPF"
     gene            13025..13258
                     /locus_tag="ACHJWA_003243"
     CDS             13025..13258
                     /locus_tag="ACHJWA_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFO4309788.1"
                     /translation="MSEYFRILQGLPDGPFTRKHAEAVAAQYRNVFIENDHGEQFRLV
                     VRNNGAMVWRTWNFEDGAGYWMNHVIRDFGIIK"
     gene            13324..15282
                     /locus_tag="ACHJWA_003244"
     CDS             13324..15282
                     /locus_tag="ACHJWA_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFO4309789.1"
                     /translation="MSVTESKAKTERKSSRKPAKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELADSIKGVGLLQNLVVHALPGDRYGVAAGGRRLAALNMLA
                     ERDIIPADWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADTFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEHLREAEGWEWCAGRMEPVGFCREDAGTYRSLPEPEAVLTEAEE
                     ERLNELMARYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDVADNDYRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQSD
                     KSLALLAWTLCLNVFGSGAYSNPARIRLECEHYSLTSDAPSGKEGAAFMAMMAEKARL
                     AALLPDGWARDMTTFLSLSQEVLLSLLSFCTACSIHGVQTREYGHTSRSPLDTLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEHHMKD
                     NRWVPGWMCAPRPQTDATERTDNLADAA"
     gene            15337..15771
                     /gene="psiB"
                     /locus_tag="ACHJWA_003245"
     CDS             15337..15771
                     /gene="psiB"
                     /locus_tag="ACHJWA_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFO4309790.1"
                     /translation="MKTELTLNVLHTMNAQEYEDIRAAGSDARRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDTQGYSVNDIIHILMAEGGQV"
     gene            15768..16529
                     /locus_tag="ACHJWA_003246"
     CDS             15768..16529
                     /locus_tag="ACHJWA_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFO4309791.1"
                     /translation="MSARSQALVPLSTEQQAAWRAVAETEKRRNQGNTLAEYPYAGAF
                     FRCLNGSRRISLSDLRFFMPSLTAEELHGNRLQWLYAIDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFCVRERSRHKSALVMQKYSRQQAREAEQKARAYQALVAQAEIELAFHSPE
                     TVGSWYARWSDRVAEHDLETLFWQWGERFPSLAGMERWQWQDMPFWQVIAEASLAAKE
                     AGHAVREMERWMVPNKLREVRDAATITRIARKQPE"
     gene            16703..16852
                     /locus_tag="ACHJWA_003247"
                     /pseudo
     CDS             16703..16852
                     /locus_tag="ACHJWA_003247"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5431 family protein"
     gene            16794..16919
                     /locus_tag="ACHJWA_003248"
     CDS             16794..16919
                     /locus_tag="ACHJWA_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HFO4309792.1"
                     /translation="MLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK"
     gene            17053..19074
                     /locus_tag="ACHJWA_003249"
                     /pseudo
     CDS             17053..19074
                     /locus_tag="ACHJWA_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            19142..19573
                     /locus_tag="ACHJWA_003250"
     CDS             19142..19573
                     /locus_tag="ACHJWA_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity TolA C-terminal
                     domain-containing protein"
                     /protein_id="HFO4309793.1"
                     /translation="MVLRSIWVEEQYMGVKKIVILAILVSFVAGCAPLHPSNCHKTTA
                     LGSCSSGRWDDQDEWGEQARGIRDAINAKLDDPQKWKRKKCRLHMEFSRDGTALKIST
                     SNGDKAYCEAIKSAAHKAKFPAFNNPEVYRDFQKSGFDMRG"
     gene            complement(20203..20552)
                     /locus_tag="ACHJWA_003251"
                     /pseudo
     CDS             complement(20203..20552)
                     /locus_tag="ACHJWA_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            20646..20909
                     /locus_tag="ACHJWA_003252"
                     /pseudo
     CDS             20646..20909
                     /locus_tag="ACHJWA_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            20949..21069
                     /locus_tag="ACHJWA_003253"
                     /pseudo
     CDS             20949..21069
                     /locus_tag="ACHJWA_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            21066..21218
                     /locus_tag="ACHJWA_003254"
                     /pseudo
     CDS             21066..21218
                     /locus_tag="ACHJWA_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            21325..>22011
                     /locus_tag="ACHJWA_003255"
     CDS             21325..>22011
                     /locus_tag="ACHJWA_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcdB toxin N-terminal helical domain-containing
                     protein"
                     /protein_id="HFO4309794.1"
                     /translation="MIHPGSSLDKAINNTRVKNVSTDVKHGQIQERKRNFIYKKNDDI
                     SSRFKLYSSLVKQKNATEDVVLIGKMILDEVRSYRTIHNDRNIVSNSGNWKTSFLCNL
                     ARLLYSIFNGSNYFCSREGENNSSPSSTLLTIHQPEKQELLQQKSIKHLPTSNNIDGY
                     IKIRKTRGAEDQTTTITQSLIINELLNGVDRNTIPFQKISELNDIIHSYENMQIKNSR
                     KGIEILVKQGE"
BASE COUNT         5157 a         5452 c         6329 g         5073 t
ORIGIN      
        1 acccggggcg gttcacttcc atgacgacag gatagtctga gggttatctg tcacagattt
       61 gagggtggtt cgtcacattt gttctgacct actgagggta atttgtcaca gttttgctgt
      121 ttccttcagc ctgcatggat tttctcatac tttttgaact gtaattttta aggaagccaa
      181 atttgagggc agtttgtcac agttgatttc cttctctttc ccttcgtcat gtgacctgat
      241 atcgggggtt agttcgtcat cattgatgag ggttgattat cacagtttat tactctgaat
      301 tggctatccg cgtgtgtacc tctacctgga gtttttccca cggtggatat ttcttcttgc
      361 gctgagcgta agagctatct gacagaacag ttcttctttg cttcctcgcc agttcgctcg
      421 ctatgctcgg ttacacggct gcggcgagcg ctagtgataa taagtgactg aggtatgtgc
      481 tcttcttatc tccttttgta gtgttgctct tattttaaac aactttgcgg ttttttgatg
      541 actttgcgat tttgttgttg ctttgcagta aattgcaaga tttaataaaa aaatgcaaag
      601 caatgattaa aggatgttca gaatgaaact catggaaaca cttaaccagt gcataaacgc
      661 tggtcatgaa atgacgaagg ctatcgccat tgcacagttt aatgatgaca gcccggaagc
      721 gaggaaaata acccggcgct ggagaatagg tgaagcagcg gatttagttg gggtttcttc
      781 tcaggctatc agagatgccg agaaagcagg gcgactaccg cacccggata tggaaattcg
      841 aggacgggtt gagcaacgtg ttggttatac aattgaacaa attaatcata tgcgtgatgt
      901 gtttggtacg cgattgcgac gtgctgaaga cgtatttcca ccggtgatcg gggttgctgc
      961 ccataaaggt ggcgtttaca aaacctcagt ttctgttcat cttgctcagg atctggctct
     1021 gaaggggcta cgtgttttgc tcgtggaagg taacgacccc cagggaacag cctcaatgta
     1081 tcacggatgg gtaccagatc ttcatattca tgcagaagac actctcctgc ctttctatct
     1141 tggggaaaag gacgatgtca cttatgcaat aaagcccact tgctggccgg ggcttgacat
     1201 tattccttcc tgtctggctc tgcaccgtat tgaaactgag ttaatgggca aatttgatga
     1261 aggtaaactg cccaccgatc cacacctgat gctccgactg gccattgaaa ctgttgctca
     1321 tgactatgat gtcatagtta ttgacagcgc gcctaacctg ggtatcggca cgattaatgt
     1381 cgtatgtgct gctgatgtgc tgattgttcc cactcctgct gagttgtttg actacacctc
     1441 cgcactgcag tttttcgata tgcttcgtga tctgctcaag aacgttgatc ttaaagggtt
     1501 cgagcctgat gtacgtattt tgcttaccaa atacagcaat agtaatggct ctcagtcccc
     1561 gtggatggag gagcaaattc gggatgcctg gggaagcatg gttctaaaaa atgttgtacg
     1621 tgaaacggat gaagttggta aaggtcagat ccggatgaga actgtttttg aacaggccat
     1681 tgatcaacgc tcttcaactg gtgcctggag aaatgctctt tctatttggg aacctgtctg
     1741 caatgaaatt ttcgatcgtc tgattaaacc acgctgggag attagataat gaagcgtgcg
     1801 cctgttattc caaaacatac gctcaatact caaccggttg aagatacttc gttatcgaca
     1861 ccagctgccc cgatggtgga ttcgttaatt gcgcgcgtag gagtaatggc tcgcggtaat
     1921 gccattactt tgcctgtatg tggtcgggat gtgaagttta ctcttgaagt gctccggggt
     1981 gatagtgttg agaagacctc tcgggtatgg tcaggtaatg aacgtgacca ggagctgctt
     2041 actgaggacg cactggatga tctcatccct tcttttctac tgactggtca acagacaccg
     2101 gcgttcggtc gaagagtatc tggtgtcata gaaattgccg atgggagtcg ccgtcgtaaa
     2161 gctgctgcac ttaccgaaag tgattatcgt gttctggttg gcgagctgga tgatgagcag
     2221 atggctgcat tatccagatt gggtaacgat tatcgcccaa caagtgctta tgaacgtggt
     2281 cagcgttatg caagccgatt gcagaatgaa tttgctggaa atatttctgc gctggctgat
     2341 gcggaaaata tttcacgtaa gattattacc cgctgtatca acacagccaa attgcctaaa
     2401 tcagttgttg ctcttttttc tcaccccggt gaactatctg cccggtcagg tgatgcactt
     2461 caaaaagcct ttacagataa agaggaatta cttaagcagc aggcatctaa ccttcatgag
     2521 cagaaaaaag ctggggtgat atttgaagct gaagaagtta tcactctttt aacttctgtg
     2581 cttaaaacgt catctgcatc aagaactagt ttaagctcac gacatcagtt tgctcctgga
     2641 gcgacagtat tgtataaggg cgataaaatg gtgcttaacc tggacaggtc tcgtgttcca
     2701 actgagtgta tagagaaaat tgaggccatt cttaaggaac ttgaaaagcc agcaccctga
     2761 tgcgaccacg ttttagtcta cgtttatctg tctttactta atgtcctttg ttacaggcca
     2821 gaaagcataa ctggcctgaa tattctctct gggcccactg ttccacttgt atcgtcggtc
     2881 tgataatcag actgggacca cggtcccact cgtatcgtcg gtctgattat tagtctggga
     2941 ccacggtccc actcgtgttg tcggtctgat tatcggtctg ggaccacggt cccactcgtg
     3001 ttgtcggtct gattatcggt ctgggaccac ggtcccactt gtattgtcga tcagactatc
     3061 agcgtgagac tacgattcta tcaatgcctg tcaagggcaa gtattgacat gtcgtcgtaa
     3121 cctgtagaac ggagtaacct cggtgtgcgg ttgtatgcct gctgtggatt gctgctgtgt
     3181 cctgcttatc cacaacattt tgcgcacggt tatgtggaca aaatacctgg ttacccaggc
     3241 cgtgccggca cgttaaccgg gctgcatccg atgcaagtgt gtcgctgtcg ccggcctcct
     3301 cacccggtca cgtttcgtcg ttcctcctcc acgcgctgcg gcttcggggc cgcacctgca
     3361 ttcgtatgcg gtcgcccggt tacaggtgcg gcacggcctg atggaggccg catgtgagag
     3421 gagaattccc atgccaaact ggtgctcgaa tcgtatgtat ttttctggtg aaccggcaca
     3481 gatcgctgag attaaacgac tggccagcgg tgcagtcaca ccgctttatc gccgcgccac
     3541 aaatgaaggt attcagctgt ttctggccgg aagtgccgga cttctgcaga ccactgaaga
     3601 tgtgcggttt gaaccatgcc ccggactgac ggctgccggg cgtggcgttg tatcgccgga
     3661 gaatatcgcg ttcacccgct ggctgacaca cctgcaggac ggtgtacttc tggatgagca
     3721 aaactgcctg atgctgcatg aactctggct gcagagcggt actggccggc gtcgctggga
     3781 agaattaccg gatgatgcca gggaaagcat taccgctctt ttcaccccaa aaagaggtga
     3841 ctggtgcgac atctggagta acgaggatgt atctgtgtgg tggaaccgtc tgtgtgacaa
     3901 cgtcctgccg gaaaaaacca tgccgtttga cctgctgacg gttctgtcca ctcgcctgga
     3961 tgttgaagtg aatggcttta acggtggtgt tctgaacggt gttccttctg catatcactg
     4021 gtatacggaa cagtatggcg tgaagtggcc ttgtgggtat gaggtgaata tcagcagtca
     4081 gggagacaac ttcattcagg ttgatttcga cacgccgtgg tgtcagccgg aaagcgacgt
     4141 tattgcagta ttaagccgcc gtttcagttg catgctggag cactggtacg ccgggcaggg
     4201 ctgcaatttc tgtggctggc agcgatatga gcgtggagag cttgttgatg tgctgtgggg
     4261 ggaactggaa tggtcttccc cgacagatga tgatgagctg ccggaagtca ccgcgccgga
     4321 gtggatagtc gacaaggtgg cgcattacgg tggatgaagt atgacggaga cgggcgggca
     4381 accgcccgtt tcttttccgg taaaggatgt cgccgggctc ctctttttac tgcgccggct
     4441 gacgcggcgc ggcaggaacg ctgcctgtgg tcagtgtccc gcgtccggcc gggcacggga
     4501 cgggcgcttt taccgctccc ggctggcatc ggtgacagtg tacgccagcc cgtcgccctt
     4561 ttctgatgaa cgcccttcaa gccgctttcg cggcatattc tcgccgtcaa aaagacgacg
     4621 gctgcgatat tccacggtcg gcctgacccg ttaccggacg cggtgaacag cccacaggcc
     4681 gcggggaacg ggcgccacag gggcgccctc ccggtgccct ctgtaagaga aggagtgctg
     4741 ttatgtcccg ttttatccag ggtgactgcg tgcgtgttat ggccacgatt ccgggcaatg
     4801 gtgtcgattt catcctcacc gacccgccat atctcgtcgg tttccgtgac cgtcaggggc
     4861 gtaccatcgc cggcgataaa accgatgaat ggctgcaacc ggcctgtaat gaaatgtacc
     4921 gcgtgctgaa aaaagatgcg ctgatggtga gcttctatgg ctggaaccgc gtcgatcgct
     4981 ttatggccgc ctggaaaaat gcgggattca gcgtggttgg ccacctggtg tttaccaaaa
     5041 cctacacttc gaaggccgca tatgtgggct atcgccacga atgcgcttac atcctggcaa
     5101 aagggcgtcc ggcgctgcct cagaagccat tgcccgacgt gctgggctgg aaatattcag
     5161 gcaatcgtca tcacccgacg gaaaagcctg ttaccagcct gcaaccactg attgagagct
     5221 tcacacaccc gaacgcgatt gtgctggacc cgtttgcagg cagcggctca acctgtgtgg
     5281 ccgcccttca gtccggacgc cggtatatcg gtatcgagct gcttgaacag tatcaccgtg
     5341 ccggacagca acgtctggct gccgtacagc gggccatgca gcagggggcc gcaaatgatg
     5401 actggtttat gccggaggct gcgtaaatga actatgccgg acatgaaaaa cttcgcgccg
     5461 acgtggcgga ggtggccaac accatgtgtg accttcgggc caggctgaac gatatggagc
     5521 atcgctgtcg ctttgattcc gatgtgcttg ttgaacgcct ggcacgccag acgctgtatc
     5581 gcgccaatcg cctgttcatg gaggcgtata ccgaaattct ggaactggat gcgtgcttta
     5641 aagattgaga aggagacgag aatgtacgga acatgcgaaa cgctatgccg ggagctggca
     5701 gcaaagtatc cgggatatac accgctgatg ctggttatct ggtccccgga agagattcag
     5761 gccctcgctg acggaatgga tatttccctg tccgatcctg aaatcaggac cgtcctggcg
     5821 cgcctggagg acatcccgga agaccagcga attgagtccg gtatttcttc tgccgcggcg
     5881 atggagatta tcagcaacgt gagcgaaaac cgccaggtga ccgtcccggc tgaactgctg
     5941 gcgtccctga ttcagaccgc cgaacaggca ctgtggaaac gtgaatgggc cgcccgtgat
     6001 tacggtctcg ccgtcccgga atgcgttacc cgccgtcagg cggtggttaa tcaggcccgc
     6061 atcctgctga aaaacaacac acacgaaaac ggctgatgtt atcgccgcct gcgggcggcg
     6121 atacagggag aacgattaat catgaacgaa aacacaacgc tgaacgcact gatttgtcgt
     6181 cacgcccgta acctgctgct ggcgcagggc tggccggaag agacggatgt cgaccagcgt
     6241 aacccgaagt atccgggctg gatcagcatt tatgttctgc tggatgcgcc ccggctggcg
     6301 acgttactcg ttaaccgcca cggcggcgta ctgccgccgc acctggcctc cgccattcag
     6361 aaactgaccg gaaccggagc ggaacttgtt ctgtccggca gtcagtggca gtcgctgccg
     6421 gtgcttccgg cagacggaac gcaggtgtct tttccgtatg ccggtgaatg gctgaccgaa
     6481 gacgaaatca gggctgtact tgcggcggtg cgcgatgcgg tacgcagtgt cagttgccgg
     6541 gtggcagaag atacgcggcg tatccgggcg gcgctgacca ccaccggtca gacgttactg
     6601 acccgccaga cgcgccgctt tcgcctggtc gtgaaggaaa gcgatcaccc ctgctggctc
     6661 gatgaggacg acgaaaacct gcccgtggtg ctcgatgcca tcctgaaccg gggcgcacgt
     6721 ttttcggcgg tggaaatgta tctggtcagc gattgtattg aacatatcct gtccagtggg
     6781 ctggcctgcg atgtgctgcg tataccggat gaaccgcccc gccggtggtt tgaccgtggt
     6841 gttctgcggg aggtggtccg ggaagcccgg aacgaaatcc gcagcatggc ggatgccctg
     6901 gcaaaaatcc ggaaatgatg acgttggcgg ggtaaatccc cgcctttttc ctgccggtcc
     6961 ccggacagag cacaaaggaa gtcgccgctg gctccgcttt acccggccat gcagggcatg
     7021 gccttgtggt ttttcagtta tgtggcctca gcgtcgtgtg cgggcggtgc cgtgcctcca
     7081 tcttagccgg gctggcgttg atgcaagggt acgcttcgcc gctgcggtca cccggtccct
     7141 ccttcccgtc tgccgtgatt ttccggtcgt tcgcccgctc aggtttcgcg gcctcaccct
     7201 gcaactcccg tcagccgtgt gcgtcaggct gcggcttccc ttgcgccctg tgcatccccg
     7261 ccttatcgcc cggcttttct ggaggcacgg caccgcccgg tgtcgcggaa tatgtgaaca
     7321 acggaggaaa acatcatgca atatgcgaaa cctgtcactc tgaatgttga agagtgcgac
     7381 cgtctctcct ttctgcctta cctgtttggc aacgattttc tgtatgccga ggcgtatgtg
     7441 tacgcgctgg cgcaaaaaat gatgccggaa tatcagggag gcttctggca cttcatccgc
     7501 ctgccggacg gtggcggtta catgatgccg gatggcgacc gtttccacat ggtgaacggt
     7561 gcaaactggt ttgaccgtac cgtgagtgct gatgcctgcg gcatcattct gacttccctt
     7621 gtgattaacc gccagttgtg gctgtaccac gacagcggtg atgcaggact gacccagctt
     7681 taccggatgc gcgatgcgca gttgtggcgt cacatcgaat ttcaccctga atgcaacgcg
     7741 atttacgcgg cactggactg attaacggac ggggcggcaa tgccgcccct gtaaaggaga
     7801 cgagaacatg tactgtactg ttaaagaaat catccgcgat gtactggata cagacgtgcc
     7861 ggacagtgaa tgcgtttttg ccgtggtgct gacccgtggg gatgtgcgcc acatagccca
     7921 ggactggaac ctgtcagacg atgagctgga aaccgtcatg cagcggctgg acgatgcctt
     7981 tgaatatggt gcggatgtca gcattgttca cgacgttgtt cgtgagttga tggaagaaaa
     8041 gcgtgccagc cgtcaggtga cagttccggc ggtgatgctg gaaaaagtga tggcgctggc
     8101 aggcagtgaa atgaagcgcc tgtatgccgt cgggagtgag aacgggggcg acggtgatgc
     8161 gttcgtcagg gaggaacgcg aagcaatgga cgttgtgtta caggcgctgg acggggagca
     8221 catgtcatga atatcagcac agaaacccgc gaaattctgc gcaattacaa agccgtgatt
     8281 aatgcgcgac gtcgtgaaat ggggcagaaa ccgctcacca ccgcgcagat tgttgatgaa
     8341 atctgcgatt ttgtggcgaa tcagcaggct gttttcctgg gcggtcacta catcttacag
     8401 ggcagcagaa acaggtgata cagggctggc ggtggaggcc gcctgtcagc ccgcgcgaaa
     8461 acccctggca ggctgacgcc tgccggaagg gaagcgggcc acgctgacgc gtcgtccgtt
     8521 acccttcctg tttgtgatgt gtccggaatg tttttttctg atgttatttt tctgtggtta
     8581 ccgtgcctgt tactgttctt cttctttttc ctcttcctct tcctcctctt ctttccgttc
     8641 ctctcctgct tatggctttt ccgggcgtcc ccctggcggg gcgggctgtc gtgaacggag
     8701 ccagcgaagc tgtctccgcc ctggcgggct tccatccctg acgcgaaacc ctggcttgca
     8761 gcgaccgttc cggtcgcgca gcctctgtcg cgccgtggcc tgcgctcccg gcatcccggc
     8821 aaaaggccgc cgggatgtaa ggggcaacgc cgtttcgcaa accgcgcttc acgtcgttgc
     8881 ggcgggtccc tgctcattgc ctgccgggtg ccacacaccc gtatgagtcc ctgtcgccgg
     8941 gagctgatac agccacaacc tgccgcactg actgtccagg gtaaatgaac agcccacaaa
     9001 attttttacc cagaacccgg taaaaaattt tgcgttctgc ccaggacagg tgtgtcaggc
     9061 cgtggcagtg atgccccttg cggcgaaggt tctcaaacgg gtgaagttca taccacaggg
     9121 aatacagcag caggttcccg cgaaacgtca aaaccgcggc tcccgaaact caacgggtga
     9181 tgttctttaa aaatcaggat ttatttcagg ctccggcaat acgcatttat gtgttctgtc
     9241 agtcatccgg ctgacaggta atgagaacgc aggaacagaa ggaggaaaac accggaaagc
     9301 gtcagatgca gatatgaaaa aagcaggtca gggacctgct taatccacgc cgtaagaatt
     9361 gtgcgaccaa ttttgacggc attgttaacc aatttgtcat aaggaatcca tttatgactg
     9421 taagcattgt ttcaccttcc gcagcggctg taaagccccg ccgtcatccc cgtttcagac
     9481 gcgaagacat ccccgctccg gaaattgacc ccgtgctgaa agccttcggg cgacacatag
     9541 cccggagttt tcaccggggg cgtggtgtac acatccccgc catgaaaaac acggctttcg
     9601 ggcaggtgct ccgcacactc gaactgaaac gcgcttttaa ctgatgaggt gagccgcccc
     9661 gcgaggggcg gcaacagacg gaaggagaac attttatgac atcagcagca atcaccatga
     9721 ccgccccgga agccgccagc cctgtgcaga tgtaccgcgc gacctactca ccggatgaca
     9781 acaaactgcg cctgtatgcc gtgtcacgtc ttgacccgga gacgtataaa aaagtgcatg
     9841 atgccggttt tcgctgggca ccaaaacagg cgctgtttgt cgcgccggcc tggacaccgg
     9901 gccgggaaga cgtgctcctc tcacttgccg gagagattga ggatgaagac agcacgctcg
     9961 ctgaacgtca ggaagcacgg gcggagcggt ttaccggata cagcggaaag cgggccagtg
    10021 aatccgcaca ggcacttgat gaagtggaaa gactggccgc gatgatcccg cccggtcagc
    10081 ccattcttgt ggggcatcac agcgaacgcc gcgcccgtcg tgatgcgcag cgtattgaaa
    10141 acggcatgaa acgtgccgtg atgctctttg aacgtgcgga atactgggaa gaacgggggc
    10201 ggtcagcact gcttcacgcg aagtataaag aacgtccgga cgttcgctgg cgtcgtatca
    10261 aaaaaatcga agctgatttg cgcaaggctg aaaagaccat cgcgcagtcg cagaaatatc
    10321 tgacgatgtg gcgggctgaa tcgctggatc tgaatatggc aaaactcatc agcagtcatg
    10381 accatatcag cgcctgtttc ccgctggata cgtatccgcg cccggcagaa aaaagccagt
    10441 atgaagggag ccggtcgtta tggtcggccc tggatgatga catcatcacc acggagcagg
    10501 cccgcgaaat tgcgatccgc tgtcatgaac ggcagattca gcatcagcaa cgctgggtta
    10561 accactatca gaaccgcctg aactatgagc gtgccatgct ggacgaaagc ggcggcgtgg
    10621 ttacccggac acaggatttt gagccgggcg gacaggtttt cagccggggc gagtggctga
    10681 ccatcatccg cgtgaacaaa agcaacgggg cggtgagttc agtcacaacg ccgaattaca
    10741 gttttctcgg gtacagcggc acgatgaaag tgacgcccga tcgcatcacg gactacaaag
    10801 caccatcggc agaagaggct gccgtcgcca gccaggccgc gaagcgtccg ccggtagtca
    10861 actatccggg ggaaggtttc cgggaaatga caaaggcaca gtgggccgcc ctgccccggg
    10921 actgtaaggc cgtgcgcagt gtggcagaag cagaagacca cggggcatac cgctaccgcc
    10981 gcacaatgga caataatttc cgtctggtga atgtgtatat caccgacatg aaaattacgg
    11041 aaatcccaca gaaataaggt atatcccccc gggaaatccc cgggggatga cggagataat
    11101 gacatgcaca gtcagttaaa agaacgtatc cggctgatgc gcgcaaggct ggataacgcc
    11161 gcgccggttg ctgaaatccg ggctgaatct cagctttttg tgactcctgc cccggtctgt
    11221 gatcgcctgg tgacgctggc ggagatcagc aaccgtgatc acattctgga accctctgcc
    11281 ggcaccgggg ccatactgcg ggcgattcgg gataccgcac cggaggccat gtgtgatgcg
    11341 gtggaaatca acagcgggct ggtccggtat ctgcgggaaa attttaacgg tgtcagggtt
    11401 cagtgtggcg acttcatgga atggcagccg gtgcagtatt acagccgggt tatcattaat
    11461 ccgccgttca gccacgggca ggatatccgg catatcctgc gggccttttc cctgttgcgt
    11521 ccgggtggcg tgctggtcgc cgtctgtctg aacgggccgc gccagcagga gaagctgtta
    11581 ccgttttctg acgtccgcga ggagctgcca cgcggcacgt ttgcttatac cgatgttccg
    11641 acgatgatta ttcgtctgcg tgcctgatga ctccgggcag gcgggaggat aaatgcccgt
    11701 ctgcccgcgc cgggaaaaga ccgcgcgccg gtggcgctga aaggggccgc gctcacgcgc
    11761 cgccccttac attacgcgga cattctgctg tgggcttctg tcttttcaga cctgtcattt
    11821 gtctgttact gatgatgttg ttgttcatgc tactgctact gctactgcta ctgctactgc
    11881 tactgctact gctactgttc ttcttttttc tgctgctctg attactgtct gttttgttgt
    11941 tctcctgctt atgccttttc cgggcgtccc cctggcgggg cgggctgtcg tgaacggagc
    12001 cagcggagct gtctccgccc tggcgggctt ccatccctga cgcgaaaccc gggcttgcag
    12061 cgaccgttcc ggtcgcgccg cctctgtcgc gccgtggcct gcgctcccgg catcccggca
    12121 aaaggccgcc gggatgtaag gggcaacgcc gtttcgcaaa ccgcgcttca cgtcgttgcg
    12181 gcgggtgcct gcccgttgtt gtgtccggtg ccattcacct gtttaagccc ctgtcgccgg
    12241 gggcaaagac agccacaacc gtctccgcca gtccaggggt gaaatgcaca tcccgcaaaa
    12301 ctttttgccc aaaagcaggc aaaaagtttt gcggtctgcc cccggacagt cgtttccggc
    12361 cgtggcagtg atgccccttg cggcgaaggt tctcaaaaag gtgatggtga ataccggagg
    12421 gaacaaaagg caggtccccg caaaacgtca aaaccgcggc tcccgaaact caaaagatga
    12481 tgggggctga aaacatcaac agaaggagac acatcatggc agttcgtggc attaacaagg
    12541 tcatcctcgt aggacgcctg ggaaaagatc cggaagtccg ttacatcccc aacgggggcg
    12601 cagtggcaaa cctgcaggtg gccacgtcag aaagctggcg tgacaaacag acgggggaga
    12661 tgcgggagca gacagaatgg catcgcgtgg tgctgttcgg caagctcgcg gaagtggcag
    12721 gtgaatatct gcgcaagggc gcgcaggtct acatcgaagg tcagcttcgc acccgtagct
    12781 gggaggataa cggtatcacc cgttacgtca ccgaaattct tgttaagacc acgggcacca
    12841 tgcagatgct ggtacgtgcc gcaggtgctc agactcagcc ggaagagggg caacagttca
    12901 gcggtcagcc tcagccgcaa ccgccggagg gtgacgatta cgggttttca gacgatatcc
    12961 cgttctgaac gactgactgt gacaaccgcc ccgtcctgtg cggggcatca ccggagagat
    13021 gaggatgagc gaatatttca gaatacttca gggactgcct gacggtcctt ttacccgcaa
    13081 acatgccgaa gccgttgccg cacagtaccg gaacgtcttt atcgagaatg accacggcga
    13141 acagtttcgc ctggttgtcc gtaataacgg tgcaatggtc tggcgtacct ggaattttga
    13201 ggacggtgcc gggtactgga tgaaccatgt catccgtgat ttcgggatta ttaagtaaga
    13261 gaggtgccgg acgggaacgc gtccggcaga caatcattat taccgttttg ggaggtacta
    13321 actatgtcag ttacagagtc taaggcaaaa acggagcgta aatccagccg taaacctgca
    13381 aaaacgcagg aaacagtcct gtcggccctg ctggcgcaga cggaggaagt gagcgtgccg
    13441 ctggcctcgc tgattaagtc accgctgaat gtgcgcacgg tgccgtattc tgcggagtcc
    13501 gtcagcgaac tggcggactc cattaaaggt gtcggcctgt tacagaatct ggttgttcat
    13561 gccctgccag gtgaccgtta cggtgtcgcc gcaggtggtc gccgactggc agcactcaac
    13621 atgctggcag agcgtgacat cattccggct gactggcctg tccgtgtgaa ggtcattccg
    13681 caggagctgg cgactgccgc atcgatgacc gagaacggtc atcgtcggga tatgcaccct
    13741 gccgaacaga ttgccggatt ccgtgcaatg gcgcaggaag gcaaaacacc tgcacaaatc
    13801 ggtgatttgc tgggttattc gccccgccac gttcagcgaa tgctgaaact ggcagacctt
    13861 gcgcctgtca tcctcgatgc gctggcagaa gaccgcatca ccaccgagca ctgtcaggcg
    13921 ctggcgctgg agaacgacac cgcgcgtcag gtgcaggtgt ttgaagccgc ctgtcagtcg
    13981 ggatggggcg gtaaaccgga agtacagacc attcgtcgtc tggtgaccga aagtgaagtg
    14041 gcggtggcag ggaacagtaa attccgcttc gtgggggctg ataccttctc gccagatgaa
    14101 ctgcgcaccg atttgttcag tgacgacgag ggtggctatg tggactgcgt ggcgctcgat
    14161 gccgccctgc tggaaaaact ccaggctgtc gccgaacacc ttcgggaagc cgaaggctgg
    14221 gaatggtgcg ccggacgtat ggagcctgtt ggtttctgcc gtgaggatgc cggaacatac
    14281 cgcagtctgc cggagccgga agcggtgctg acggaggcag aagaagaacg cctgaacgaa
    14341 ctgatggcac gttacgatgc gctggaaaac cagtgtgagg aatccgacct gctggaagcc
    14401 gaaatgaagc tgatgcgctg catggcgaag gtcagggcgt ggacgccgga gatgcgtgcc
    14461 ggaagtggcg tggtggtgtc ctggcgttat ggcaacgtgt gtgtccagcg tggtgtgcag
    14521 ttgcgcagtg aggatgatgt ggctgataac gattaccgca cggaacaggt gcaggagaaa
    14581 gcctcagtgg aggaaatcag tctgccgctg ctgacgaaaa tgtcctcaga gcgcacgctg
    14641 gcagtccagg cggcactgat gcagcaatca gacaaatccc tggcacttct ggcatggaca
    14701 ctctgtctga atgtatttgg cagtggagcg tacagcaatc ccgccagaat ccgcctggaa
    14761 tgtgagcatt attcgctgac cagcgatgcg ccgtcaggga aggaaggtgc cgcattcatg
    14821 gcgatgatgg cagaaaaagc ccgtcttgcc gccctgctgc cggatggatg ggcacgggat
    14881 atgacgacgt tcctgtcact cagtcaggag gtgctgttat cactgctcag tttctgcacc
    14941 gcgtgcagta tccacggtgt ccagacccgt gagtatggtc acacgtcacg cagtccgctg
    15001 gacacgctgg agagcgccat cggctttcac atgcgcgact ggtggcagcc gacgaaagca
    15061 aactttttcg gacacctgaa aaagccgcag attatcgcag ccctgaatga ggcaggacta
    15121 tccggtgccg cacgggacgc ggagaagatg aagaaaggcg atgcggctga acatgcagag
    15181 caccatatga aagacaaccg ctgggtgcct ggctggatgt gtgcaccacg cccacagacg
    15241 gatgccactg aacgcaccga taacctggct gatgccgcct gatgaacaac cacaccgccc
    15301 cgccggagac ggggcggcag caagggagat accgtgatga aaactgaact gactctgaat
    15361 gtattacata ccatgaacgc acaggaatat gaagatatcc gggctgccgg aagtgatgca
    15421 cgccgtgagc tgacacacgc cgtgatgcgg gagctggatg cgccggacaa ctggacgatg
    15481 aacggcgagt acggcagcga gtttggcggc tttttccccg tccaggtccg tttcacgcct
    15541 gcccacgaac gttttcacct ggcgttatgt tcaccgggcg atgtctccca ggtctgggtg
    15601 ctggttctgg tgaatgccgg tggtgagccg tttgcggtgg ttcaggtaca gcggcggttt
    15661 gcaccggaag ccgtcagcca ttcactggca ctggcggcgt cactggatac gcagggatac
    15721 agcgttaacg acatcatcca tatcctgatg gcggaaggag gtcaggtatg agcgcacgtt
    15781 cacaggcact ggttcccctc agcacagagc aacaggccgc atggcgggca gtggcagaga
    15841 cggaaaaacg ccgcaatcag ggaaacacgc tggcggaata tccgtatgcg ggggcgtttt
    15901 tccgctgtct gaacggaagc cgcaggatat cgctgtctga cctgcgattt ttcatgccgt
    15961 ccctgaccgc agaagaactg cacggaaacc gtcttcagtg gttgtatgcc atcgatgtgc
    16021 tgattgaaac acagggcgag gtctgcctcc ttccccttcc cggtgatgca gcagaacgct
    16081 tgttcccgtc ggtgcggttt tgtgtcaggg aacgcagtcg ccataaatcc gcgctggtca
    16141 tgcagaagta cagccgccag caggcgaggg aggcagagca gaaagcccgt gcgtaccagg
    16201 cgctggtcgc ccaggctgaa atcgaactgg cgttccattc accggaaaca gtcggaagct
    16261 ggtatgcacg ctggagtgat cgggtggcag agcatgacct ggaaaccctg ttctggcagt
    16321 ggggcgagcg ttttccgtca ctggccggaa tggaacgctg gcagtggcag gatatgccat
    16381 tctggcaggt tatcgcggaa gccagtctgg cagcaaagga ggccggtcat gctgtccgtg
    16441 agatggagcg gtggatggtg ccgaacaaac tccgggaggt gcgtgatgcg gcaacaatca
    16501 cacggattgc ccgtaaacag cctgaatgag cgggttattt tcaggaaaag tgaatgtggt
    16561 cagcgtgctg gtatatgggc tatgatgtgc ccggcgcttg aggctttctg cctcatgacg
    16621 tgaaggtggt ttgttaccgt gttgtgtggc agaaggacaa aagccccgta gttaattttt
    16681 cattaaccca cgaggcccct gcatgcttag acaacatcag gatagcctct taccgcgctt
    16741 tgcgcaagga gaagaaggcc atgaaactac cacgcagctc tcttgtctgg tgtgtgttga
    16801 tagtgtgtct cacactgttg atattcactt atctgacacg aaaatcgctg tgcgagattc
    16861 gttacagaga cggatacagg gaggtggcgg ctttcatggc ttacgaatcc ggtaagtagc
    16921 aacctggagg cgggcgcagg cccgcctttt cagggctgat gctggtctga ctgcactgaa
    16981 gcgcctttat aaaggggctg ctggttcgcc ggtggcccct ttctccttgc tggcattgta
    17041 cgggcatgaa cagcctggcg acgcgggaaa aagcagacgt tcagggaata tattgctcag
    17101 atgtcgattg ccgggctgcg tgaagagccc gcacaggact ggttatcttt gcataaacgg
    17161 ctggccagtg atggtctgta tatcacgatg caggaagggg aactggtggt gaaggatggc
    17221 tgggacagag cgcgggaagg tgttgcactc agttcgttcg gaccgttatg gacagctgaa
    17281 aaactggggc ggaaactggg ggaatatcag cctgtaccga cagatatttt cagtcaggtg
    17341 gggacacccg gtcgttatga tccggaagcc ataaacgttg atatccggcc ggaaaaagtg
    17401 gcagaaacgg agagtctgaa acagtacgcc tgccgtcatt ttgccgaacg cttaccggaa
    17461 atggcgagaa atggtgagct ggaaagttgc ctggatgtgc acagaactct ggcaacggct
    17521 ggattatgga tggggatcca gcacgggcat ctggtactgc atgatggatt tgataaacaa
    17581 cagacaccag tacgtgcaga cagtgtgtgg ccgttaatga cgctggatta tatgcaggat
    17641 ctggatggtg gatggcagcc tgtaccgaag gatattttta ctcaggttat acccggagaa
    17701 cggttccggg gacgtaatct tggtactcag gctgtcagtg attacgaatg gtatcgtatg
    17761 cggatgggga caggtccaca gggagcaata aagcgcgaac tgttttctga taaggaaagt
    17821 ctgtgggggt acacaactgt tcagtgtgag tcgttgattg aagatatgat cgcgggcggg
    17881 aatttcagct ggcaggcgtg ccatgagatg tttgcgcgta aagggcttat gttgcagaaa
    17941 cagcatcatg gtcttgtgat tgtggatgcg tttaatcatg agctcacacc agtgaaggcc
    18001 agcagtatcc acccggattt gacgttatcc agggctgagc cgcaggcagg gccatttgag
    18061 attgcaggtg cagatatatt tgaacgggtg aagcctgaat gtcgctacaa tccggaactt
    18121 gctgccagcg atgaagtgga gcccggcttc cggcgcgatc ctgtactgcg tcgtgaacgc
    18181 cgtgaagccc gtgcagctgc gcgggaagat ttgcgcgcgc gttatcttgc atggaaggag
    18241 cactggcgta aacccgactt acgttacgga gaacggttac gggaaattca tgctgcctgc
    18301 cgtcggcgca aggcgtatat ccgtgtgcag ttccgggatc cacaattgcg taagctgcat
    18361 taccatattg cggaagtgca gcgtatgcag gcgctgatca ggctgaagga gagtgtgaag
    18421 gaagagcggt tgtcactgat tgaggagggt aagtggtatc ctttgtccta ccggcagtgg
    18481 gtggagcaac aggcggtaca gggagacaga gctgcattat cacagttacg tggctgggat
    18541 tatcgtgatc gtcgcaaaga caagcgacga acaacgaatg ccgaccgctg cgtgattctc
    18601 tgtgaaccgg gaggaactcc actttatgaa gacactggcg ttctcgaagc ccgtctgcag
    18661 aaagatggca gtgtgcgttt tcgtgatcgg aggaatggcg agttagtctg tgtggattat
    18721 ggggaccgtg tggtgttcta ccatcatcag gacagaaatg aactggtgga taagctgaat
    18781 ctgattgctc ctgtattgtt tgatcgtgag ccaggaatgg gttttgaacc ggaaggctca
    18841 tatcaacagt ttaatgatgt atttgcggaa atggtggcct ggcataatgc agctggaata
    18901 accggaaatg gacactttgt aatcagccgc ccggatgtgg atttacatcg tcagcgtagt
    18961 gaacagtact accacgaata tatcaggcaa cagaaaagca tatccggggg gcatggcgca
    19021 tcttatgctc cagttcagga taatgagtgg acgccaccat cacctggaat gtagggatga
    19081 ggagtggata ttaaaaatct taacttgtat tagttaagat tttctttgct ctgacagagc
    19141 tatggtactg agatctatat gggttgagga gcaatatatg ggggtaaaga aaatcgttat
    19201 actggctatt ttagtcagct ttgtggcggg atgtgcgcca ttacatcctt caaattgcca
    19261 taaaactaca gccctgggta gttgcagttc aggacgttgg gatgatcagg atgaatgggg
    19321 ggagcaggca cggggaatca gggatgcaat taatgctaaa cttgatgatc cgcaaaaatg
    19381 gaaaaggaaa aaatgcaggt tgcatatgga attctcccgg gatggcacgg cgttaaaaat
    19441 atcaaccagt aatggcgata aagcctattg cgaagcgata aagtcagcag cccataaagc
    19501 taaattcccg gccttcaaca atccggaagt ttatagagat tttcaaaaat ccggctttga
    19561 catgcgcggt tagctacacg acatatctgt agggctaaca gaagactgtc aggtcggctg
    19621 attactggaa cctgaggtac tttgcctcag gttatgttga catactcaaa aaaagattct
    19681 tacattatcc cggtaacggc gtccttcatc ggcctgtccg ttaaactcag gctacctcac
    19741 acctcacacc tcacacctca caacagcggg tctggacaga aagcggctct tgatgtggat
    19801 tatcgcatgg tgtgggcttt cagcggcatg gtgactggaa agtaacgata ttgcatctgt
    19861 ggcaacgaaa attgcagagt tggtactctt tgaactgcat aagtaagcag aaaggtaagg
    19921 ttatgctttg cgattgaaat tcagccttaa cgtacaataa ttttcgatat ccaaactgcc
    19981 cccctataac agcaaaaggt cccacaagac gctcgtggaa tatattttaa aagagaacaa
    20041 aaattgcaat atgtggtggc tccatacaga ggattgacaa cattcccatg ctaattgtta
    20101 agattcaaat tgaagtatat ctatctgtca tttttgcaaa tgacatgcgg tagcataacg
    20161 ttccgggggc tcctgtgccg tcatgcgtga cgacattgat cagccaataa tggcacgtga
    20221 ccacagccag atacggccag ccttcatccg tacgtaaata tgtgatgtct cccgcccact
    20281 tctggttcgg gccactggcg taaaaatcct gctccagcag attttctgac acaggctggc
    20341 tgtgtgcgcg gtaatagacc ggactgaaat tccaggaagc ttttgccctc agtccgtgac
    20401 ggcgcaggct ttccgtcacg gtttttactt taaaatggta accctgggca cgcagttcat
    20461 ccatcaggcg ttgtgcatga ccgggtaaaa gcaacgagga caacgctgtc gcagtgctga
    20521 cggaactgcc gacgtgtact tatccttatc cgccagtgat ataagacggt aattcaccat
    20581 ttggattgtc cgctccaccc aacatgttgt ttccttgagg ttctcacacc agaaaggaca
    20641 tcagcatgct gagcagagag gacttttaca tgataaagca aatgcgccag caaggtgcgt
    20701 atattgttga tattgcgact cagattggtt gctctgaacg aacggtcaga cgttacctga
    20761 aatacccaga acctccggcc agaaaaacac gccacaaaat ggccgaaatg gactatatcg
    20821 acatgcgtct ggctgagaat gtctggaacg gcgaagttat cctggcggaa atcaaagcga
    20881 tgggttatac cggtggccgt tccatgttgc ttcgccagtt caggaagaca ccggagcagc
    20941 gctttacgca ggaacacagt ctgttcttcc ggttgctgaa ccgtcgatat gaaaaagcga
    21001 gcattgtgct gacgtccaat aaagggttcg ccgactgggg gagagatgtt cggtgataac
    21061 gtgctggcaa aatattcacc caacgcattg agcgtaataa tctgacgcta cgcacccgca
    21121 ttaagcggtt gactcgtaaa accatctgct tctcacgctc agttgagatc cacgaaaaag
    21181 tgatcggagc gtttatcgaa aaacacatgt tctactaatt ggatgcacca ccgaatacat
    21241 taatttatat aacaacatca aaatacattt taggttgtcg ctaatcaaaa attattaagt
    21301 gttacataat tagagggtat agatatgatt catcctggct cttctttaga taaagcaatt
    21361 aacaatacaa gagttaaaaa tgttagtact gatgttaaac atggccaaat tcaagagaga
    21421 aaaaggaact ttatctataa aaaaaatgat gatatctcat ccagatttaa actttacagt
    21481 tcgctcgtaa agcagaaaaa tgcgacagaa gatgttgttt taataggcaa aatgatttta
    21541 gatgaagtta gaagttacag aactatacat aatgatcgaa atatcgtaag taactcagga
    21601 aactggaaaa catctttttt atgtaatctt gctagactac tatatagcat atttaatggt
    21661 agtaactatt tttgttcccg agagggtgaa aataattcat cccccagttc tactctactt
    21721 actatacatc agcctgaaaa gcaggaacta ttacaacaaa agagtatcaa acatttacca
    21781 acaagtaata acatcgacgg atacattaaa ataagaaaaa caagaggcgc tgaagatcaa
    21841 acaacaacta tcactcaaag tttgataatt aatgagttgt taaatggagt tgatagaaat
    21901 accatccctt ttcagaaaat aagtgagctc aatgatatca tacattcata tgaaaatatg
    21961 caaattaaaa atagtcgaaa aggtatagaa atacttgtta agcagggaga g
//
LOCUS       DAZFAB010000076        21876 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZFAB010000076 DAZFAB010000000
VERSION     DAZFAB010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21876
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.076"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            251..751
                     /gene="tssB"
                     /locus_tag="ACHJWA_003256"
     CDS             251..751
                     /gene="tssB"
                     /locus_tag="ACHJWA_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFO4309795.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSSLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDEMNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GQVSNK"
     gene            786..1010
                     /locus_tag="ACHJWA_003257"
     CDS             786..1010
                     /locus_tag="ACHJWA_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4309796.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            1061..2452
                     /gene="tssC"
                     /locus_tag="ACHJWA_003258"
     CDS             1061..2452
                     /gene="tssC"
                     /locus_tag="ACHJWA_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFO4309797.1"
                     /translation="MSLQEEELVSSHAGQPEQESSLLDQIMAQTRIQPGSEGYDVARQ
                     GVTAFIASILQSTASAEPVNKLAVDSMIADIDERISRQMDVIIHAPAFQQVESFWRSL
                     KTMVDRVDFRENIKVNVLHVTKQELLEDFEFAPEIIQSGFYKHVYSSGFGQFGGEPIA
                     AVLGAYEFKNTAPDMKLLQYVSAVGAMAHAPFLSSVSPEFMGLNSWTELPNIKDLYAI
                     FEGPAYTKWRALRDSEDSRYLGLTAPRFLLRQPYSPTDNPVKNFNYYEDVSQNHEDYL
                     WGNTAWMLACNIADSFAKYRWCPNIIGPQSGGAVKDLPVHLFETMGQIQAKIPTEVLV
                     TDRREFELAEEGFITLTMRKDSDNAAFFSANSVQKPKHFPGKDAETNYKLGTQLPYLF
                     IINRLAHYIKVLQREQLGSWKERSDLERELNTWIRQYVADQENPPADVRSRKPLRAAK
                     VEVMDVEGEPGWY"
     gene            2543..2956
                     /gene="tssE"
                     /locus_tag="ACHJWA_003259"
     CDS             2543..2956
                     /gene="tssE"
                     /locus_tag="ACHJWA_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFO4309798.1"
                     /translation="MILDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            2960..4810
                     /gene="tssF"
                     /locus_tag="ACHJWA_003260"
     CDS             2960..4810
                     /gene="tssF"
                     /locus_tag="ACHJWA_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFO4309799.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQMEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWSLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            4774..5856
                     /gene="tssG"
                     /locus_tag="ACHJWA_003261"
     CDS             4774..5856
                     /gene="tssG"
                     /locus_tag="ACHJWA_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFO4309800.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLDHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTTFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            5881..7161
                     /gene="tagH"
                     /locus_tag="ACHJWA_003262"
     CDS             5881..7161
                     /gene="tagH"
                     /locus_tag="ACHJWA_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HFO4309801.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHAPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDHNDDISAAEMAQRHLAVTPLLRGLGGSLTVSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7158..7682
                     /gene="tssJ"
                     /locus_tag="ACHJWA_003263"
     CDS             7158..7682
                     /gene="tssJ"
                     /locus_tag="ACHJWA_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFO4309802.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLQAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPIKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7685..9016
                     /gene="tssK"
                     /locus_tag="ACHJWA_003264"
     CDS             7685..9016
                     /gene="tssK"
                     /locus_tag="ACHJWA_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFO4309803.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPDQDALPDPLHPQNFPDERSRNIY
                     LALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDARTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHRARRSQLHPEDFYLDLAELLGELMTF
                     TEPSRLPCPLDVYDHHDLTKTFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADVH
                     DSELLQSATFVLAVAANMPVDQIQRQFIQQSKISSPEKIRNMVSVQIPGIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            9021..9782
                     /gene="icmH"
                     /locus_tag="ACHJWA_003265"
     CDS             9021..9782
                     /gene="icmH"
                     /locus_tag="ACHJWA_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFO4309804.1"
                     /translation="MDEGSLSLPPFTGYDEKSLRDYHLALHGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWLCFSLGFR
                     GRYKVAVQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9791..12568
                     /gene="tssH"
                     /locus_tag="ACHJWA_003266"
                     /gene_synonym="clpV"
     CDS             9791..12568
                     /gene="tssH"
                     /locus_tag="ACHJWA_003266"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFO4309805.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTLTDASDTLLARYAKNMTEDARNGRLAPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYERSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQTLVREIIALRQQLLGVAEDD
                     AAPLPDADTVEDTQPESESESESEQDNTGAVPADETDREQPEETAETVSPVQRLAQLT
                     AELDALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLPKWLGDTIKG
                     QDLAIASLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQYLT
                     TINMSEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDVLN
                     LFYQAFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEALYPVLADF
                     FKPALLARMEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGAEVVIEPEVTDEIMSR
                     VTRAENGARMLESVIDGDMLPPLSLLLLQKMAANTAIARIRLSAVDGAFTADVEDAQN
                     DESVTKDETVL"
     gene            12565..13308
                     /gene="vasI"
                     /locus_tag="ACHJWA_003267"
     CDS             12565..13308
                     /gene="vasI"
                     /locus_tag="ACHJWA_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFO4309806.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDHLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADSRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            13313..14725
                     /gene="tssA"
                     /locus_tag="ACHJWA_003268"
     CDS             13313..14725
                     /gene="tssA"
                     /locus_tag="ACHJWA_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFO4309807.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPDWEYIDGEIVK
                     LGSLAHAQLDIPELQRRGLQLLASESKDFRLLAHLLRTLQHAGDPLLALHLLTLYVEH
                     YWTVAAPQNMAHKKRFASQIIKRFETGIEGFSQNAATTQRDTLLGELAKLAQCWQSHN
                     VPELAQATDDLFALYQRTFNRAAPAPVPTPAASGSSPQTTVTSESGVTQPSAPAPQIA
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQSITSTPQAESDGRTPLAA
                     VSADMVADYQSRLASADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADTIR
                     DEVIRFLERLPQLTGLLFNDRTPFLSEQTKQWLAASPDGKVAPVAQIGEESQAARACF
                     AGQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLIQLAQQQYRMLLMIGSQMM
                     VSDWEPSLLTQLEQKFTAEQ"
     gene            14862..18296
                     /gene="tssM"
                     /locus_tag="ACHJWA_003269"
     CDS             14862..18296
                     /gene="tssM"
                     /locus_tag="ACHJWA_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HFO4309808.1"
                     /translation="MLILAWIFLLVWIWWKGPTWTLYEEQWLKPLANRWLATAAWGII
                     ALVWLTVRVMKRLQLLEKQQKQQREEAIDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGTEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLTADKRHREHLL
                     QTLRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVTQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     SLFPQALLAEPNLATESRAWLMRSRRRLTVFSATGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPQGEDDYGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKAMNAAPPESEEKLAVLRVMRMLEDKSGRNNE
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKP
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFLTRYGLQSYFVKQRDELVELTAMDSWVLNLTRSVKYSDADRAEIQRQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPGVFSEKLSA
                     KERDEALAEPDYQLLTRLGHEFAPENITLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLTDQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNNVIIREDIIAQLETAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGNAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            18307..19716
                     /locus_tag="ACHJWA_003270"
     CDS             18307..19716
                     /locus_tag="ACHJWA_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HFO4309809.1"
                     /translation="MNSNVLTQTIVTGSDPRGLPEFSAIREEINKASHPSQPELNWKL
                     VESLALAIFKANGVDLHTATYYTLARTRTQGLAGFCEGAELLAAMVSHDWDKFWPQGG
                     PARTEMLDWFNSRTGNILRQQISFAESDLPLIYRTERALQLICDKLQQVELKRVPRVE
                     NLLYFMQNTRKRLEPQLKSNTENAAQTTVRTLIYAPETQASSTPEAVVPPLPGLPEMK
                     VEVRSLTENPPQASVIKQGSTVRGFIAGIACSVAVASALWWWQVYPVQQQLLQVNDTA
                     QGAATVWMASPELENYERRLQQLLDTSPVQPLETGMQMMRVADSRWPESLQQQQASTQ
                     WNEALKTRAQSSPQLRGWLQTRQDLHAFADLVMQREKEGLTLSYIKNVIWQAERGLGQ
                     ETPVESLLTQYQDARAQKQNTDALEKQINERLEGVLSRWLLLKNNTIPTIKKALNFNN
                     IHEYKGVLNGEFNLFNTKW"
     gene            19682..20161
                     /locus_tag="ACHJWA_003271"
     CDS             19682..20161
                     /locus_tag="ACHJWA_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFO4309810.1"
                     /translation="MANLIYLTLNGDNQGLISSGCSSQPSIGNKAQTAHIDQIMIYEF
                     MHGLNRDQNVNHHHLTIKKPIDKASPLLGKAICDNELLTCDFSFYRTNKFGINELFYK
                     IKLTGAKISDIHVSISHIVVDNSVQPEESVSFSYESIIWEHCSAGTSAYSLWEDRLF"
     gene            20182..20403
                     /locus_tag="ACHJWA_003272"
     CDS             20182..20403
                     /locus_tag="ACHJWA_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_302948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4309811.1"
                     /translation="MANNILHSSTSDSESKNTSTDSTLFNEKFPFQLRQDFASRITED
                     ESDFFKWKKIRELAFVSNHKEWNKYDLLI"
     gene            20482..21078
                     /locus_tag="ACHJWA_003273"
     CDS             20482..21078
                     /locus_tag="ACHJWA_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HFO4309812.1"
                     /translation="MLWTENDAENTSQWNGYPLQIGRFRKDKAMPALISGEKSTALVT
                     PPQWRNKAFNGLKDPERNYWAKEQITGSPEENIKAAITYLMMKLSNTKEESTIDQYDS
                     TLYSAIVQKGDLADNIRKERKTTIPNLTKNNPGKNLDKIHPGDILYYQKASMKVIITG
                     WKPITIKNVAMNYNGGGDPKYAIKLQFVYTLLTKNRVL"
     gene            21081..21530
                     /locus_tag="ACHJWA_003274"
     CDS             21081..21530
                     /locus_tag="ACHJWA_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HFO4309813.1"
                     /translation="MRKITIILLLTFSCTKGLAFDISMSKECSGVDELEFIDCVKNSF
                     IMSTITLKKAEANIKEKIQKWNSDEDEAIRKEAVISLKRLESSNREFIRYRFAQCSFT
                     LTWGARVGRLAFSRLYPCYTELNKIRAMQLNNAYISDFQNPDEISSK"
BASE COUNT         5437 a         5800 c         5829 g         4810 t
ORIGIN      
        1 ttaaattatt gccacaatac acctgacgcg aattgcacag gttcataaaa tctatttaac
       61 gcttgcagaa tacccctctt tacgatcgat aaaaagtcct tttcttattt attttgaagc
      121 gtaaaatgcg gggtgatgaa ggagtggtga ttttattttc accgccatca agacggcatt
      181 tccgatattt tttgcggtac gaatatgccg tgttcatggc ctgtacgaga taacatgacg
      241 gatcactttt atgagcaaaa aatttgaagg cagcgtcgcg cctcgcgaac gtattaatat
      301 atcgtatgtc ccaaaaacag atggccagac ggctgaagtg gaacttccgc ttaacatgct
      361 cgttgtcggc gataccggaa atacgcagga aacatcctca ctggatgaac gtcaggcagt
      421 ttccgttaac aagcacaatt tcggtgcagt gatggctgaa gcagccattg gcctcaattt
      481 taccgtcccc gccacgctga agggaagcac aacggacgat gaaatgaatg tggcgctgaa
      541 tatcaagtca ctggatgatt tttcaccaga tagcgtggcc cggcaggttc cggaagtgaa
      601 taaattgctg gaactgcgtg aagcactgac tgcactgaag gggccgatgg gtaaccttcc
      661 ggcattccgt acccagctcc aggcattgct ggaaaatgaa gagtcccgcg agcagttgct
      721 caaagaaatc ggccaggtga gcaataaata accaccacta tttcattaaa cagggttatc
      781 caggcatgac ggcacacaaa aatatctccg ggacatacca cctctctgtt gcagacattc
      841 tgcaggtggt ttaccaggta tgtttcagcc cgtccgttga aatcaaccag gatggtgtgg
      901 ctgcgctgat tacaacactg gatcgccgta tcagtgatct attggatgag attatccatt
      961 tttgtgagtt tcagcagagc gcgtcgcact ggcagcgcgt acttcactga cggcgaaaag
     1021 ccggtttcag aaacacagct aaaacagaag gattttcagt atgtctttac aggaagagga
     1081 actcgtttcc agtcatgccg ggcagcctga gcaggaatcg tcgctgcttg atcaaatcat
     1141 ggcacagacc cgtattcagc ccggttcgga aggttatgac gttgcccgcc agggtgtgac
     1201 agcctttatt gccagcatcc tgcaatccac tgcatctgcc gaaccggtca ataaactggc
     1261 cgtggacagc atgattgcag atattgatga acgcatcagc cgccagatgg atgtcatcat
     1321 ccatgcgcct gcctttcagc aggtcgagtc gttctggcgc tcactgaaaa ccatggtgga
     1381 tcgcgttgat ttccgtgaaa acatcaaggt caacgtgctt catgtcacca agcaggaact
     1441 tctggaagac tttgagtttg cgccagaaat tattcagtcc ggattttata agcacgttta
     1501 ttcgtccggt ttcggacagt ttggcggtga acccatcgct gccgtgctgg gcgcttatga
     1561 gttcaaaaat accgcgcctg acatgaaact gctgcaatac gtcagtgccg tgggcgcaat
     1621 ggcgcacgca ccgttcctgt cctccgtttc cccggagttt atggggctga actcgtggac
     1681 cgaactgcct aatatcaaag atctttatgc catctttgaa ggtccggcct acaccaaatg
     1741 gcgcgccctg cgtgactcgg aagattcgcg ctatttaggg cttacggcac cccgcttcct
     1801 gcttcgccag ccctattcac caacagataa ccctgttaag aactttaact attacgaaga
     1861 tgtcagccag aaccacgaag attatctgtg gggtaatacg gcctggatgc tggcatgcaa
     1921 tatcgcagac agttttgcca aataccgctg gtgtccaaac attatcggtc cgcaaagcgg
     1981 cggcgcagtg aaagatctgc cggtgcatct gttcgaaacg atggggcaga ttcaggccaa
     2041 aatcccaacc gaggtgctgg tcaccgatcg ccgcgaattt gaactggctg aagagggttt
     2101 tatcaccctt accatgcgta aagactctga taatgcagcc tttttctctg caaactcggt
     2161 acaaaaaccg aagcactttc cgggaaaaga tgcagaaacc aactataagc tgggtacgca
     2221 gcttccttat ctcttcatca tcaatcggtt agcgcactac atcaaggtgt tgcagcgtga
     2281 acaactgggg tcatggaaag aaaggagcga cttagaaaga gagcttaata cctggatccg
     2341 tcagtatgtt gccgaccagg aaaacccgcc tgcagacgtg cgcagccgca aacctctgcg
     2401 cgctgccaaa gttgaggtta tggatgtaga aggcgaaccc ggctggtact aggttgcgct
     2461 aagcgtgaga cctcatttta aatttatggg ggcaaatttt gagctttccc tggttggccg
     2521 gttagacagg gagtaacaca ccatgatact ggatcgggaa agaaccacag gttctctttt
     2581 tgagcgtatg gaagcctctt cggccagaaa ccggcaggga ggcagtattc attcgctcag
     2641 gcaatccatt cgccagaacc taagaaacat tcttaatacc cggtccggta gttgccgggg
     2701 tgcgcctgag ctgggcattg atgaaccgga aggggctgaa aacttccgcg aatccatgag
     2761 tcgggccatc gagcaatgta tagaacgcta tgaaccccgg atctcacacg ctgaagtgca
     2821 ggcagtggtt tcatccgcct ccagcccgct ggatatgacg tttcatatta cagcctgggt
     2881 gacatttaac gaaacgcatg aggtgcttga atttgatatg gcaccgaatg gcagccagca
     2941 ttaccgggtg gactgacaaa tggaatttga agaacgctat tttcgggaag aactcgatta
     3001 cctgcgccag cttagcaagc tgctggcaac ggaaaaaccc catctggccc gcttcctggc
     3061 cgaaaaagat gcggatccgg atattgaacg cctgctggaa ggggtggctt ttcttaccgg
     3121 caatctccgc cagaaaattg aggatgaatt cccagaactg acgcacgggc ttattaagat
     3181 gctatggcct aattacctgc gtccggttcc ggcaatgacc cttattgaat atacgccgga
     3241 tatggataag tcttctgtac cggtgttaat cccccgtaat gagcagttta caaccaacgc
     3301 cggggaaatc agagttgatg aagtgctgcc ctctgatgct aaaaaggagg agccgcctcc
     3361 ctgtaccttc accctctgcc gggatatctg gctgctgccc gttcgcctgg agcagattga
     3421 aaaccgcagt acgacccgta atggtgttat caacatcacc ttttcggtcg caccgggaac
     3481 ggacttccgc acgctggatc tgaacaaact tcgcttctgg ctcggcaatg acgacaacta
     3541 tacccgtgac cagctttatt tatggttctg cgaatacttg cagggtgccg acctgactgt
     3601 gggtgaacag catattcgcc tgcctgagtt tatgctaaaa gctgtcggtt ttgagccgca
     3661 ggatgccatg ctgccctggc cgaaaaacgt ccacagcggc taccggatcc ttcaggagta
     3721 tttctgttac cccgatgcgt ttctcttttt tgatctttgt ggttgtccgg ctttgcctga
     3781 cggattgcag gcggaattct ttaccctgca actgcgtttt tcgcgccctt tgcccgtgga
     3841 catccggctg cgccgcgatt ccctgcgcct gtattgcgca cctgccatta atttatttat
     3901 ccaccatgca gaagccatca cgctggacaa ccggcgggca gactatccgc tggttcccag
     3961 ccgccattac ccacaacatt acgatgtatt ttccgttaac agtgtggtga gccaggtcca
     4021 ggatatgttc aggaaaaaag atctggggcg tcctgtttcg acgcaggccg cgcgccagtg
     4081 gccagccttt gaaagtttca gccatcagat ggaatacagc cggaagcggg aggtggtgta
     4141 ctggcatcac cggaccaaaa catccctgtt ccatcgcggc tttgatcata cccttgcctt
     4201 tatacatgct gatggcagtt atccgtcaga cgaatctctg ctcagtaatg aagtggtttc
     4261 ggtatcgctg acctgtacca accgtgagct tccgtcacaa attcgttccg gcgatatcac
     4321 cggcacaacc ggtaaaaatg cggctgttgc ttcctttcgc aacattaccc gcccgacgca
     4381 accactctgg ccggtcattg atggcagcct gcactggtcc ctgctctccg ccatgaacct
     4441 gaattatctg tcattactgg atacggacgc gctgaagcag gtcatcgcca actttgatcg
     4501 ccacgcaatc caccatccgc agacggcgcg gctgtcacaa caaaagctgg atgccattga
     4561 gcgtctggag acccgccccg ttgatcgcct gtttacgggt attcccgtcc ggggactggc
     4621 ctccacgctg tatctgcacc cggagccgtt tgtctgtgaa ggggaaatgt atctgctcgg
     4681 tacggtgctt tcgcattttc tgtcgctgta cgccagcgtt aactcattcc acatgctgac
     4741 cgttgtgaac acagaaagcc aggagacatg gaaatggacg gaaagaatcg ggcagcatcc
     4801 tcttatctga gtccgggaaa tcccccggca gataaagagc agaatgatcc gctggcacag
     4861 gttttccata atgcctgctc ttacaatttt tttgcgatgg cggagctgtt gcaccgcctg
     4921 gcaaagggtg aaaaggggac gccagaatta tccctgcgcg acgatccggc acaggaaacc
     4981 ctgcgtttca gcgccgatgc cagccttgcc ttcccgtgca gtgatatcag tgcgctgaaa
     5041 cgggatacgt caggggcatt caggatgacc actaccttta tggggttaca ggggagccag
     5101 tcgcctctcc ccggttatta cctcgatcac ctggcctgga aagcggtaca tgaacaaagt
     5161 ccggtaggtg attttctgga tatgttcagc caccgcctga cccagtttgt ctggcatatc
     5221 tggcgtaagt accgctatca catcagtttt cgtaacggcg gtgtggatgc gttctcgcag
     5281 cgcatgtact cccttgtcgg gctgggccac cgccagcttc gcgataagct ggcgattaat
     5341 cacagcaaaa tgctggcgta ttccgggatc ctggcaaatc cgggacgatc cccggaaatt
     5401 atctgtggcc tggtgtccca ctgctttgat ttgtcagaag tgacgctgca aaactggcaa
     5461 cgtcgcaagg tggatattga accggatcag caaaacagcc tggggagcta ttctcttaaa
     5521 aatggcgaaa aactggcggg aagatcggta ctgggcaact ttgttctggg tacacgagta
     5581 cccgatctca gcggaaaatt ccagctcagt attaccagcc tgacccgaaa acagttcctc
     5641 tcttttctgc cgtccggcga aaacttcctg ccactgacga cgttcgtgtc cttcatcctg
     5701 cgcgatcagc ttgcctggga cttacatctg gggctggcgc cggagcaggt gggcgcaatg
     5761 cgcctgggcg ataacaaaag tgcgctgctg ggctggacca gcttcctcgg cacaccggaa
     5821 gaacgaccct cagtcaccat cagggtacgg tcgtaaatta tgataatcac ggagcagaat
     5881 atgccggaag agaaactaca gacgctttca ttgcaggtca ttaacggcag tgagctggaa
     5941 agcgggcggg cggcgcgctg tctgttcaca cagcagggaa atgtcggcca tgcccccgaa
     6001 tgccactggt cggtacagga tcgtcagcag agcattccgg cccaggcttt taccgttatc
     6061 ctgcacgatg gcacattttg tctacgcccg cagacggcac aactgtggct gaatcaggca
     6121 aaagtcacag caacatcaga cctgatacag ttgcgccagg gcgatgagat ccagatcgga
     6181 cggctgatgg tgagggttca tctgaaccgg ggagatattc cccattacga tgaggaaatg
     6241 gccactcccg aaaccatcgt taccaatcgc gatatgctca cggataccct gctatcaacg
     6301 gagggtgcgc cacactatcc gggaatgact caccggcacc agcttgcaga caccgtggta
     6361 aatggttttt ctgccgatcc actccaggca cttcagtccg aaagcctgat taccacgggc
     6421 gatccgcttt caggcattgc ggctgtccgg ccatcggcac cgctgtccga tccggcaagt
     6481 aatgggggga tcaatactcc gtttatggat ctgccgccca tttatgccag ccctggcgat
     6541 cataatgatg acatctctgc ggcagaaatg gcgcaacgcc accttgcggt caccccctta
     6601 ctgcgcggtc ttggcggctc gcttaccgtg agcaattccg acgatgcgga tgattttctg
     6661 gaggaggccg gacgaacgtt acaggccgca ataaaaggtc tgctcgattt gcagcagcag
     6721 cgtaacagcc tctcagacaa acatttgcgc ccgctggaag ataacccgct gcgcctgaac
     6781 atggattacg ccaccgcgct cgacgtgatg tttgccgaag gtaaaagccc ggtacatctg
     6841 gcggctcccg ccgccgtcag tgaaagcctg cgcaatgtcc gccaccacga agaagctaac
     6901 cgggcggcga ttgtggagtc gcttcgtgtc ctgctggatg ctttctcacc acaaaatctg
     6961 ctgcgccgct ttgtgcagta ccgccgcagc catgaactgc gccagccgct ggatgatgcc
     7021 ggagcatggc aaatgtacag ccattattac gaagaactgg cctccgatcg ccagcagggg
     7081 tttgagatgc tgtttaacga ggtctacgcc caggtctatg accgggtgct tcgtgaaaaa
     7141 cagcgggagc cggaagcatg aacaggaagg cgtttctggc ctgcgtgctg atgtgcgtca
     7201 tattaaccgg ctgcgaaacg gcgaaaaaaa tcagcgaggt gatcaaaaac ccggatatcc
     7261 aggtcgggag cctgaaggag caaccttcgg aaataaccgt caccctgctg actgaaccgg
     7321 acaccaatac caatgcggag ggtgaatcag ccgccgtgga tgtgcaactg gtgtacctga
     7381 ctgacgactc aaaattgcag gcagccgact atgaccagat agccagtacg ccgctaccgg
     7441 atgtgctggg taaaaactat atcgaccacc aggatttcaa cctgttgccg gacaccatca
     7501 aaaccctgcc gccgataaag ctggatgaga aaacgcagtt tattggtgtc gtggcctatt
     7561 tctccgatga ccaggccact gaatggaaac aaatcgagac ggtggaagga accggccacc
     7621 attaccgcct gctggtgcat gtacgtcaaa gcagcatcga aatgaaaaaa gaggatgaat
     7681 aattatggca accacccgca acaaggtgat gtggcaggaa gggatgctga tgcgcccaca
     7741 ccatttccag caacagcagc gttacaacga ctacctggat aaccagcgtt tccgggccat
     7801 gaatgattta tcctggggat ttaccgaact caccctcaac aatgaactgc tggcgcaggg
     7861 taagatcatg attgacagcg cgtcaggcac actgcctgac ggcaccgtct tttctatccc
     7921 cgaccaggac gcactgcccg atccgctgca cccgcaaaat tttccggacg aaagaagccg
     7981 caatatttac ctcgctctgc cggtcgccag tgatgtgaga aatgaaatca gcgacgggcg
     8041 gcgaatcggg cgttaccggc tgaattatgc cgatgtccgg gatctgcatt cagaagaagg
     8101 cgacgcgcga acactgacgc tgggacaact gacgccgcgc attatgagcg gtgcagaaga
     8161 tatgagtgcc tatattacgc tcccgctttg ccgtatcagc gatcgccatg ccgacggctc
     8221 cctgacgctg gatgacgatt ttatcccctc ctgccagaat attcaggtca gtaagaaact
     8281 gcgtgtttat ctcaaagagg tacagggggc cattggcgga cgggcaagcg atctggcaaa
     8341 ccgtattggc tctccggcgc agagcggcat cgcggatgtg gcggaattta tgatgttgca
     8401 gttgcttaac cgtaaccaga cccggtttac ccatcgcgcc cgccgatccc agctccaccc
     8461 ggaagatttc taccttgatc ttgccgagtt gctgggtgaa ctgatgacct ttacagagcc
     8521 gtcacgcctg ccctgcccgc ttgatgtgta tgatcatcat gacctgacca aaacatttaa
     8581 aacactgtta ccggaagtca aacgggcgct gcataccgta ctgtcgccaa gagcggtcaa
     8641 tctgccgctg catctgcgtg acggcatctg gcaggccgat gttcatgact cggaactgct
     8701 acagtctgcc acctttgtac tggccgtggc agcaaatatg cctgtcgatc agatccagcg
     8761 tcagtttatc cagcagtcga aaatttcctc gccggaaaaa atccgcaata tggtcagtgt
     8821 gcagatcccg ggtattcctc tgcgcgcact gatggtggcc ccccgccagc ttccctacca
     8881 ttccgggttc agctatttcg aactcgacaa gagcggccag gcctggacag aaatggctgc
     8941 cgccggggcc gttgcactgc atgtatccgg cagtttcccg gatctgaaca tgcaactgtg
     9001 ggcgataaga gggtaagacg atggatgagg ggtcactgtc tctgccacca tttaccggat
     9061 acgacgaaaa aagcctgcgc gactaccatc tcgcgcttca tggcaacagc ctgaatccga
     9121 tgatcgacgc ggcgacgccg ctactcggta tggtaatgcg cctttccacc atgaacagcc
     9181 agacgatgcc ggagcatctt tttgctcagg tggtgacaga tgttcaggcc gtggagcaac
     9241 tgcttcagga acagggctac gaaccgggcg tgattatctc gtttcgctac atcctctgca
     9301 cgtttattga tgaagcggcg ctcggtaacg gctggtcgaa caagaacgag tggatcaagc
     9361 agtcattgct ggtccatttc cacaacgaag cctggggtgg cgagaaagtt ttcatcctgc
     9421 tggagcgcct gatccgtgaa cccaaacgtt accaggatct gctggaattt ctctggctct
     9481 gtttttctct gggatttcgc gggcgctaca aagtggcagt acaggatcag ggggaatttg
     9541 agcagatcta ccgccgtctg taccacgttc tgcataaact gcgcggcgat gcccctttcc
     9601 cgctgttaca ccaggacaaa aaaacccaag gcgggcgtta tcagctcatc agtcgcctga
     9661 ctgttaaaca tattttttgt ggtggcgttg tcgtgctggc gttgttttac ctgttctatc
     9721 tgttgcggct ggacagccag acgcaggaca tcctgcacca gctaaacaaa ttacttcgat
     9781 aaggacgttt atgatccaga ttgatcttcc cacgctggta aaacggctga acctgttctc
     9841 ccgccaggcg ctggagatgg ccgcctcaga atgtatgagc cagcaggcag cggaaattac
     9901 cgtcagccat gtgcttattc agatgctcgc catgccacgc agtgacctgc gggttattac
     9961 ccgccagggc gatattggca tggaagagtt gcgccaggcg ttgacggtag agaactacac
    10021 aaccgcccgt tctgcggaca gctacccggc gttttccccg atgctggttg agtggcttaa
    10081 agagggctgg ctgctggcgt cggctgagat gcagcacagc gaactgcgcg gcggcgtgtt
    10141 gctgctggcc ttgctgcatt cgccgctgcg ttatataccg cctgctgccg cccggctgtt
    10201 gaccggcatt aaccgtgacc gtctgcaaca ggactttgtg cagtggacac aggagtcggc
    10261 ggaatcagtc gtgccggatg cagacggtaa aggcgcaggc acactgacgg acgcctctga
    10321 caccctgctt gcccgctacg ccaaaaacat gacagaagac gcccgtaacg gcaggctagc
    10381 cccagtactg tgccgcgacc acgaaatcga cctgatgatc gacattctct gccgccgccg
    10441 taaaaacaac ccggtggtgg tgggcgaagc aggcgtgggt aaaagcgcgc tgattgaagg
    10501 gctggcgctg cgcatcgtgg caggccaggt gccggacaaa ctgaaaaaca ccgatatcat
    10561 gacccttgac ctgggcgcat tgcaggccgg ggcatcggtg aagggtgagt ttgaaaaacg
    10621 cttcaagggg ctgatggcgg aggtcatttc ctctccggtg ccggtcattc tgtttatcga
    10681 cgaagcgcat accctgattg gcgcgggcaa ccagcagggc gggctggata tctccaacct
    10741 gctgaaaccg gcgctggcgc gtggcgaact gaaaaccatc gccgccacca cctggagcga
    10801 gtacaaaaaa tacttcgaga aagacgccgc cctgtcgcgc cgcttccagt tagtgaaggt
    10861 cagcgaaccc aacgctgccg aagccaccat tattctgcgt ggtctgtcgg cggtctatga
    10921 acggtctcac ggcgtgctga ttgacgatga tgccttgcag gccgcagcaa cgctgagcga
    10981 acgttatctc tccgggcgtc agttaccgga caaagcgatt gatgtgctgg ataccgcctg
    11041 cgcccgtgtg gccatcaacc tgtcgtcgcc gccgaagcaa atctcggcgc tgaccactct
    11101 gagccaccag caggaggcgg aaattcgcca gcttgagcgc gagcttcgca tcggactgcg
    11161 taccgacaca tcacggatga ccgaggtgct ggtgcagtat gatgaaacgc tgacggcgct
    11221 ggatgaactg gaagcggctt ggcaccagca gcagacgctg gtccgggaga ttatcgcgct
    11281 gcgccagcag ttactgggcg tggcagagga cgatgcggcg ccgttgccgg acgcagatac
    11341 tgtggaggat acgcagccag agtcagagtc agagtcagag tcagaacagg ataataccgg
    11401 tgccgtaccg gctgatgaga ccgacagaga acagccggaa gagaccgctg aaacagtttc
    11461 cccggtacag cggctggccc agctcactgc cgaactggac gccctgcata acgaccggtt
    11521 gctggtctcc ccgcacgtcg ataaaaaaca gattgcggcg gtgattgccg aatggaccgg
    11581 cgtgccgctc aaccgcctgt cgcagaatga aatgtcggtc atcaccgacc tgccgaaatg
    11641 gctgggcgac accatcaaag gccaggacct ggcgattgcc agcctgcata agcatctact
    11701 gaccgcacgc gccgacctgc gtcgtccggg acgcccgctc ggtgcgttcc tgctcgctgg
    11761 ccccagcggc gtgggtaaaa ccgaaaccgt cctgcaactg gcagagctgc tctacggcgg
    11821 tcgccagtac ctgaccacca tcaatatgtc cgagttccag gagaaacaca ccgtttcgcg
    11881 gctgattggc tcccctccgg gctatgtcgg ctatggcgaa ggcggcgtgc tgactgaagc
    11941 catccgccag aaaccctact cggtggtgct gctcgatgaa gtggaaaaag cgcacccgga
    12001 tgtcctcaac ctgttctacc aggcgtttga caagggcgag atggccgacg gcgaaggccg
    12061 cctgattgac tgtaaaaaca tcgtcttctt cctcacctcc aacctcggtt accaggtgat
    12121 tgtcgaacac gcggatgacc cggaaaccat gcaggaagcc ctctatccgg tgctggcgga
    12181 cttctttaaa cctgccctgc tggcgcgaat ggaggtggtg ccgtacctgc cgctgtcgaa
    12241 agagacgctc gccaccatta tcgccgggaa actggcccgt ctggataacg tgctgcgcag
    12301 tcgctttggt gcagaagtgg tcattgaacc ggaagtgacg gacgaaatca tgagccgcgt
    12361 cacccgcgcg gaaaacggcg cgaggatgct ggaatctgtc atcgatggcg acatgctacc
    12421 gccgctctcg ctgctgctgt tgcagaaaat ggcggctaac acggcgattg cccggattcg
    12481 gttgtcggca gtggacggcg catttacggc agacgtggaa gatgctcaga acgacgagtc
    12541 cgtcacaaag gatgaaacgg ttttatgagc aggttgcgcg ttttccggca gacaggatgg
    12601 tttatcgcgg gcctgatgac gggcttaccc gccactgccg cgcccgcaga agcatcgtca
    12661 atggccggtg tggcggtcgc cgtcgccacc acgaccccgc cggatgcgac agccacgtta
    12721 caggccatgc agtcctgtcg gcgggagtcc gcggcgctgg agcggcttga ctgctatgac
    12781 cacctgctgg caccgctgtc cccgtcaggg tttgacgggg cgctggtcaa agccggtttt
    12841 gtgggcgagg cctggacccg cgccacagag caggaaaaac gccgcgaggg caacaccaca
    12901 gaactgctgg tgacgcaggt gccgggggag cgcccgacgg tggtgataac caccccggct
    12961 atcggccatg ttccgccgcg cccggtgctg atgttcagtt gcgtggacaa tatcacccgg
    13021 atgcaggtgg cgctgatgca ccctctggat gtccatgata tcgccgtcac tctgaatgcc
    13081 gacagccggg cattgcgcag ccactggttt gtgcgcgaga acggcaccct gctggagtcc
    13141 agccgggggc tgtccgggat tgatgaaatt aagcaactgt tcggcgcaaa aacgctgaca
    13201 gtggataccg gcgcagataa cgccgcagga aagctgacct ttaacattga cgggctggca
    13261 agggcgattg cccccctgcg cgatgcctgc cactgggcgg gagaataaaa atatggcaat
    13321 ggacttacgc gatccgaatg tctggatatc gcacctgctg gaaaacctgc cggaagaaaa
    13381 actggcatcg gcgctgaaag atgacaaccc ggactgggag tatatcgacg gcgaaatcgt
    13441 caagctgggg tctctggccc acgctcagct tgatattccc gaactacaac gcagggggct
    13501 acagcttctg gcttctgaaa gcaaagactt caggctactg gcacacctgc tcagaaccct
    13561 gcaacatgcc ggtgatccac tgctggcact gcacctgcta acgctatacg tggaacatta
    13621 ctggactgtg gccgcgccgc agaatatggc gcataaaaaa cgctttgcca gccagatcat
    13681 taaacgtttt gaaacgggta ttgaaggctt ttcacaaaac gctgccacaa cgcagcgcga
    13741 tactctgctg ggtgagctgg cgaaactggc gcagtgctgg cagtcacata acgtcccgga
    13801 actggcacag gctaccgatg atctttttgc cctgtaccag cgtacgttta atcgtgcggc
    13861 tcctgctccg gtccccactc cggcggcctc cggtagttca ccacaaacca ccgtcacgtc
    13921 tgaaagcggc gtgacgcaac ccagtgctcc ggctccccaa atcgccatcg acagtcacga
    13981 cgacaaagcc tggcgcgaca cgctgttaaa agtggcggct attttatgtg aacgccagcc
    14041 ggactcgccg cagggctatc gcctgcgccg ccatgccctg tggcaatcca tcaccagtac
    14101 accccaggcg gaaagcgatg gacgtacccc actggctgcg gtctctgccg atatggtggc
    14161 ggattaccag tcccggcttg ccagcgcgga tatggcgctg tggcaacagg ttgagaaaag
    14221 cgtattgctg gctccttact ggctggacgg tcactgtctt tctgcacaga cggcactgcg
    14281 tctgggttac aaacaggtgg cagacaccat ccgcgatgag gtcatccgct tccttgagcg
    14341 tctgccccag cttaccgggc tgctgtttaa tgaccgcaca ccgtttctca gtgagcagac
    14401 gaaacaatgg ctggctgctt cgcccgacgg caaagttgca ccggttgcgc aaatcggtga
    14461 ggaatcgcag gcagccagag cctgttttgc tgggcagggt ctggaggcgg cgctgcgata
    14521 tctggacatg ctacccgaag gcgatccccg cgatcagttt caccgccagt accttgccgc
    14581 acagttgacg gaggaggcgg ggctgataca gcttgcgcag caacagtacc ggatgttgtt
    14641 gatgataggg agtcagatga tggtgtctga ctgggagcca tcattactta cgcagcttga
    14701 acaaaaattc acggcagaac aataaaagag aacaggagtt tttgtgttca gattaccaac
    14761 accccgacta ctcagcggac tcaaatcagc cctgcgaccg gcgatgcccc ggtttaaagt
    14821 ctccgctttc tggctgctga tactggcgtg gatttttctg cttgctgata ctggcgtgga
    14881 tttttctgct ggtatggatc tggtggaaag gcccgacgtg gacgctgtat gaagaacagt
    14941 ggctcaaacc actggcgaac cgctggctgg caacggcggc gtgggggatt attgccctgg
    15001 tgtggcttac cgtccgggta atgaagcgcc tgcaactgct tgaaaaacag caaaaacagc
    15061 agcgcgaaga ggccattgat ccgctcagtg tggagctgaa cgcccagcag cgttaccttg
    15121 accgctggct gctgcgcctg caacgccatc tcgacaaccg ccgtttcctg tggcagttac
    15181 cgtggtatat ggtcatcggc ccggcgggca gtggcaaaac gacactgctg cgcgaggggt
    15241 ttccgtcaga cattatttat gccccggagg gcgcgcgcgg cacagaacaa cgcctgtacc
    15301 ttacgcccca tgtcggtaaa caggcggtga tctttgatat cgacggcaca ctctgcgctc
    15361 ccgctgatgc ggatatcctg catcgccgtc tgtgggaaca tgccctcggc tggctgaaag
    15421 aaaagcgcgc gcgccagccg ctgaacggga ttattctgac actcgattta cccgatctgc
    15481 tgaccgcaga caaacgccac cgcgagcatc tgttacagac gctgcgcagc cgcttacagg
    15541 atatacgcca gcatcttcac tgccagttac cggtttacgt ggtactgacc cggcttgatt
    15601 tattacaggg ttttgccgcc ctgttccagt ccctgaacag acaggatcgc gatgcgattc
    15661 tgggcgtcac gttcacccgc cgtgcccatg aaaatgatga ctggcgaaca gagttgaatg
    15721 ctttctggca gacatgggtg gatcgaatga atctggcgtt gccggatctg atggtcaccc
    15781 agactcacac ccgcgcgtct ttattcagtt tttcccgcca gatgcaggga agccgtgaac
    15841 cgctggtgtc actgcttgag ggtctgcttg acggtgaaaa tatgaacgtg atgctgcgtg
    15901 gtgtctatct cacctcttcg cttcagcgcg ggcagatgga tgatatattc acccagtctg
    15961 ccgcccgcca gtaccgactg ggcaataacc cactggcgtc ctggcctctg gtggacaccg
    16021 cgccttattt cacccgcagc ttgttcccgc aggcattact cgcagagcct aatctggcaa
    16081 cggagagccg cgcctggctg atgcgttccc gtcgccgcct gacggttttc tctgccaccg
    16141 gcggcgtggc ggcactgctg ctcatcaccg gctggcatca ctattacaac ggtaactatc
    16201 agtccggcat caccgtgctt aagcaggcca aagcctttat ggatgtgccg cctccacagg
    16261 gggaagatga ctacggcaac ctgcaactgc cgctgctgaa cccggtacgc gatgccacac
    16321 tggcctatgg cgactggggc gaccgcagcc gtctggccga tatgggactg taccagggac
    16381 gacgtatcgg accttatgtg gaacagacct atctgcaact gctggagcaa cgttacctgc
    16441 cctcgctgtt taacgggctg gttaaagcga tgaatgccgc gccgccggag agtgaagaaa
    16501 aactcgcggt gctgcgcgtg atgcgaatgc tggaggacaa aagcggacgt aacaatgagg
    16561 tggtgaagca gtatatggca aaacgctgga gcgaaaagtt ccacggccag cgcgatatcc
    16621 aggcacaact gatgtcccat cttgactacg cgctggctca tactgactgg cacgcagagc
    16681 gtcaggcggg cgacggtgac gccatcagcc gctggacgcc atatgacaag cccgtggtat
    16741 cagcacagaa agaactgagc aaactgcctg tctaccagcg ggtttaccag agcctgaaaa
    16801 cgcgggcgct gggcgttctt cctgccgacc tcaatctgcg tgaccaggta gggccaacct
    16861 ttgaccaggt gtttacatct gccgatgaca acaaactggt tgttccacag tttcttaccc
    16921 gttacggcct gcaaagctat tttgtaaaac agcgcgatga actggttgaa ctgacggcga
    16981 tggattcctg ggtacttaac cttacccgca gcgtgaaata cagtgacgcc gaccgcgcgg
    17041 aaatccagcg ccagctcacc gagcagtata tcagcgacta caccgccacc tggcgggccg
    17101 ggatggacaa tctgaatatc cgcaattttg agtccatcgg acaactgacc ggggcgctgg
    17161 agcaggttat cagcggcgac cagcctttgc agcgggcgct gaccgtgctg cgtgacaaca
    17221 cacagccagg cgtcttttct gaaaaactct ctgccaaaga acgggatgag gccctggcag
    17281 agccggacta ccagttactc acccgtctcg ggcatgagtt cgccccggaa aacattaccc
    17341 tggcagtaca gaaagacaaa gaaagcacga tgcaggccgt gtatcagcaa ctcaccgagt
    17401 tgcaccgcta cctgctggca atccagaacg cgcctgtacc agggaaatcg gcgctgaaag
    17461 ccgtgcagtt acggcttgat cagaacagca gcgatccgat attcgccacc cgccagatgg
    17521 caaaaacgct gcctgctccg ctcaaccgct gggttggcag actgactgac caggcctggc
    17581 atgtggtgat ggtggaggct gtacattata tggaagtgga ctggcgcgac agcgtggtga
    17641 aaccgtttaa cgagcaactg gcaaataact atccgtttaa tccgcgttct gcacaggatg
    17701 cctcactgga tgccttcgaa cgcttcttta aaccggatgg catactggat accttctacc
    17761 agcagaacct gaagctgttt atcgataatg acctgagtct ggaggatggc gataacaacg
    17821 tcattattcg cgaagatatt attgcgcaac tggaaactgc gcagaaaatc cgtgacatct
    17881 tcttcagcaa acagaacggt ctgggaacat cctttgccgt ggaaacggta tcgctttcag
    17941 gcaataaacg ccgcagtgta ctgaaccttg acggtcagtt agtcgattac agccagggcc
    18001 gtaactatac cgcccatctg gtctggccta acaacatgcg cgaaggcaac gaaagtaagc
    18061 tgacgctcat cggcaccagc ggcaacgcgc cgcgcagtat cagcttcagc gggccgtggg
    18121 cgcagttccg cctgttcggg gccggacaac tgaccggagt acaggatggc aactttaccg
    18181 tgcgctttag cgtggacggt ggcgcgatga cctaccgtgt gcataccgac acggaagata
    18241 acccgttcag cggtgggttg ttcagccagt ttggtctgtc agacacactg tactgaggag
    18301 gcagggatga atagtaacgt actgacacaa actatcgtta ccggcagtga cccgcgcggg
    18361 ctgccggaat tcagcgccat ccgcgaggaa ataaacaaag ccagccaccc gtcacagcct
    18421 gagctgaact ggaaactggt ggagtcgctg gcgctggcga tttttaaagc caacggtgtg
    18481 gatttacaca ccgccaccta ctatacgctt gcccggacac ggacacaggg actggcggga
    18541 ttctgcgaag gtgcggaact gctggcggca atggtaagcc acgactggga taagttctgg
    18601 ccgcagggcg gcccggcgcg tactgaaatg ctggactggt ttaactcccg aaccggcaat
    18661 attctgcgtc agcaaatctc ctttgcggaa tccgacctac cactgatcta ccgcacagag
    18721 cgggcattgc agcttatctg cgataagctc cagcaggtgg aactgaagcg cgttccgcgc
    18781 gtggagaatc tgctctattt tatgcagaac acgcgtaaac ggcttgaacc ccagctgaag
    18841 agtaacactg agaacgccgc acagaccacg gtcagaacgc tgatttatgc cccggaaaca
    18901 caggcatctt ccacaccaga agcggtagtg cctcccctgc ccggcctgcc tgagatgaaa
    18961 gtggaagtgc gcagtctgac agagaatccc ccacaggcca gtgtgataaa gcaaggcagt
    19021 acggtaagag ggtttatcgc agggatcgcc tgttcagtgg ctgtcgcctc agcattgtgg
    19081 tggtggcagg tctatccggt gcagcagcaa ctgttacagg ttaacgacac cgctcagggc
    19141 gcagcaacgg tgtggatggc ctcacctgaa ctcgaaaact atgagcgcag gctgcaacaa
    19201 cttcttgata cctccccggt acagccgctg gaaaccggga tgcagatgat gcgtgttgcc
    19261 gacagtcgct ggccggaaag cctgcaacag caacaggcct cgacacaatg gaatgaggca
    19321 ctcaaaaccc gcgcacagag tagcccgcag ttgcgtggct ggttgcagac ccgccaggac
    19381 ttacatgctt ttgcagatct agtgatgcag cgcgagaaag agggactaac cctttcctat
    19441 atcaaaaatg tcatctggca ggcggagcgg ggactggggc aggaaacacc cgttgagtct
    19501 ctgttgacgc agtaccagga tgcccgtgcg cagaagcaga atacagatgc gctggaaaaa
    19561 caaattaatg agcgactcga aggcgtgtta agccgctggc tgctgctgaa gaataatacg
    19621 ataccgacga taaaaaaagc attgaatttc aataacattc atgaatacaa aggagtttta
    19681 aatggcgaat ttaatttatt taacactaaa tggtgataat caagggctca tttcaagtgg
    19741 ttgttcatca caaccatcca ttggaaataa agctcaaaca gcacacattg atcaaattat
    19801 gatttatgaa ttcatgcatg gattaaatcg agatcaaaac gtcaaccatc atcatttgac
    19861 aataaaaaaa cctatagata aagcatcacc cttattaggc aaagcaattt gcgacaatga
    19921 attattgaca tgtgattttt ccttttatcg tacaaataaa tttggaatta atgaactgtt
    19981 ctataaaatc aaattaactg gagcaaagat aagcgacatt catgtctcta tatcacatat
    20041 tgttgttgat aacagcgttc aacccgaaga gtcagtttct ttcagttatg agtcaattat
    20101 atgggagcat tgttccgctg gcactagtgc atacagttta tgggaagatc gtctttttta
    20161 aaaacatatg ggattattat aatggctaac aatattttac actcttcaac atcagacagt
    20221 gaaagcaaaa atacaagcac tgacagtacg ttatttaatg aaaaattccc atttcaacta
    20281 agacaagatt tcgcatcaag aataacagaa gatgaaagtg atttttttaa atggaaaaaa
    20341 ataagagagt tggcatttgt gagcaatcat aaagaatgga ataaatatga cttattaatt
    20401 taaaaatctg ttaatgaaat caacattcat ttgtccagca ctccatactt tcagccgttg
    20461 gattggtata taataaaagc catgctctgg actgaaaacg atgcggaaaa tacttctcag
    20521 tggaatggtt atccattaca aattggtcgt tttcggaaag ataaagcgat gccagcttta
    20581 atctctggag aaaaatctac agcccttgtt accccaccac aatggagaaa caaagcattc
    20641 aatgggttaa aagatccaga aagaaattac tgggcaaaag aacaaataac tggctctcca
    20701 gaagagaata tcaaagcagc aattacttac ttaatgatga aactttctaa tacaaaggaa
    20761 gaatcaacta tcgatcaata tgactcaact ctatatagcg ccattgtcca aaaaggcgat
    20821 ttggctgaca acatcagaaa agagagaaaa acgaccatac ctaacttaac gaaaaacaat
    20881 cctggaaaaa atctcgataa aatacacccg ggagatattt tatattatca aaaagcatcc
    20941 atgaaagtaa taattactgg atggaaacct atcacaataa aaaatgtagc gatgaattat
    21001 aatggtggtg gcgatcccaa gtatgcaata aaacttcagt tcgtttatac attactcaca
    21061 aaaaacagag tattatgaga atgcgaaaaa taacaattat tctactgctg acattctcat
    21121 gtacaaaagg tctggcattt gacatttcca tgtctaaaga atgtagtggt gttgatgaac
    21181 ttgaatttat tgattgtgtt aaaaactcat ttattatgag cactattaca cttaaaaaag
    21241 cagaagcaaa tataaaagag aaaattcaaa aatggaatag tgatgaggac gaagcaataa
    21301 gaaaagaagc agttatttct ttaaaaagac tagagtcttc taatagggaa tttatacgtt
    21361 acagatttgc tcaatgctct tttactctaa cctggggggc cagagtaggg cgactggcat
    21421 tctctcggct atatccatgc tatactgagt taaacaaaat aagagccatg caactaaaca
    21481 atgcttacat ttctgacttt caaaatccag atgagatatc cagcaaatga ttattataaa
    21541 tttaagatta acattactat ttatactggc cacatttaca tattcagtac aatcagacga
    21601 attcgatgat caaaccactt acacgcaata ccgttatttt tccactgcta taccggaaga
    21661 tatacgtaga tttatgttct acgtagatta ttgctttgta gatttagcat tcgatatcga
    21721 aactgactca atgacagcta agaatgatat tattaatgaa tgtactccat taaaagaaag
    21781 aaacaagaca ttaaaagaaa aatataaaaa aatgaagaga taatagcaat tctttccatt
    21841 gcagatgata ctattttaat ttatgaagaa ttaaaa
//
LOCUS       DAZFAB010000077        21588 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZFAB010000077 DAZFAB010000000
VERSION     DAZFAB010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21588
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.077"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            131..799
                     /locus_tag="ACHJWA_003275"
     CDS             131..799
                     /locus_tag="ACHJWA_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFO4309814.1"
                     /translation="MFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNKIDLLE
                     KQQAQLKSQLETIQKQQTGIISSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKYFILNN
                     GSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEFARWQQ
                     LLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSAENRQK
                     RSKK"
     gene            843..5609
                     /gene="ehaG"
                     /locus_tag="ACHJWA_003276"
     CDS             843..5609
                     /gene="ehaG"
                     /locus_tag="ACHJWA_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric autotransporter adhesin EhaG"
                     /protein_id="HFO4309815.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIAIGAETEAAENATAIGNNAKAKGTNSMAMGFGSLADKVNTIALGNGSQALADNAIA
                     IGQGNKADGVDAIALGNGSQSRGLNTIALGTASNATGDKSLALGSNSSANGINSVALG
                     ADSIADLDNTVSVGNSSLKRKIVNVKNGAIKSDSYDAINGSQLYAISDSVAKRLGGGA
                     AVDVDDGTVTAPTYNLKNGSKNNVGAALAVLDENTLQWDQTKGKYSAAHGTSSPTASV
                     ITDVADGTISASSKDAVNGSQLKATNDDVEANTANIATNTSNIATNTANIATNTTNIT
                     NLTDSVGDLQADALLWNETKKAFSAAHGQDTTSKITNVKDADLTADSTDAVNGSQLKT
                     TNDAVATNTTNIANNTSNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGTET
                     TSKITNVKDGDLTTGSTDAVNGSQLKTTNDAVATNTTNIATNTTNISNLTETVTNLGE
                     DALKWDKDNGVFTAAHGNNTASKITNILDGTVTATSSDAINGSQLYDLSSNIATYFGG
                     NASVNTDGVFTGPTYKIGETNYYNVGDALAAINSSFSTSLGDALLWDATAGKFSAKHG
                     TNGDASVITDVADGEISDSSSDAVNGSQLHGVSSYVVDALGGGAEVNADGTITAPTYT
                     IANADYDNVGDALNAIDTTLDDALLWDADAGENGAFSAAHGKDKTASVITNVANGAIS
                     AASSDAINGSQLYTTNKYIADALGGDAEVNADGTITAPTYTIANAEYNNVGDALDALD
                     DNALLWDETANGGAGAYNASHDGKASIITNVANGSISEDSTDAVNGSQLNATNMMIEQ
                     NTQIINQLAGNTDATYIQENGAGINYVRTNDDGLAFNDASAQGVGATAIGYNSVAKGD
                     SSVAIGQGSYSDVDTGIALGSSSVSSRVIAKGSRDTSITENGVVIGYDTTDGELLGAL
                     SIGDDGKYRQIINVADGSEAHDAVTVRQLQNAIGAVATTPTKYFHANSTEEDSLAVGT
                     DSLAMGAKTIVNGDKGIGIGYGAYVDANALNGIAIGSNAQVIHVNSIAIGNGSTTTRG
                     AQTNYTAYNMDAPQNSVGEFSVGSADGQRQITNVAAGSADTDAVNVGQLKVTDAQVSQ
                     NTQSITNLDNRVTNLDSRVTNIENGIGDIVTTGSTKYFKTNTDGVDASAQGKDSVAIG
                     SGSIAAADNSVALGTGSVATEENTISVGSSTNQRRITNVAAGKNATDAVNVAQLKSSE
                     AGGVRYDTKADGSIDYSNITLGGGNGGTTRISNVSAGVNNNDVVNYAQLKQSVQETKQ
                     YTDQRMVEMDNKLSKTESKLSGGIASAMAMTGLPQAYTPGASMASIGGGTYNGESAVA
                     LGVSMVSANGRWVYKLQGSTNSQGEYSAALGAGIQW"
     gene            5977..7632
                     /gene="lldP"
                     /locus_tag="ACHJWA_003277"
     CDS             5977..7632
                     /gene="lldP"
                     /locus_tag="ACHJWA_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFO4309816.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7632..8408
                     /gene="lldR"
                     /locus_tag="ACHJWA_003278"
     CDS             7632..8408
                     /gene="lldR"
                     /locus_tag="ACHJWA_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFO4309817.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTIRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8405..9595
                     /gene="lldD"
                     /locus_tag="ACHJWA_003279"
     CDS             8405..9595
                     /gene="lldD"
                     /locus_tag="ACHJWA_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFO4309818.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9781..10254
                     /gene="trmL"
                     /locus_tag="ACHJWA_003280"
     CDS             9781..10254
                     /gene="trmL"
                     /locus_tag="ACHJWA_003280"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFO4309819.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDFRAFLEAENPQRLFALTTKGTPAHSAVNYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10307..11128)
                     /gene="cysE"
                     /locus_tag="ACHJWA_003281"
     CDS             complement(10307..11128)
                     /gene="cysE"
                     /locus_tag="ACHJWA_003281"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFO4309820.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11208..12227)
                     /gene="gpsA"
                     /locus_tag="ACHJWA_003282"
     CDS             complement(11208..12227)
                     /gene="gpsA"
                     /locus_tag="ACHJWA_003282"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFO4309821.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12227..12694)
                     /gene="secB"
                     /locus_tag="ACHJWA_003283"
     CDS             complement(12227..12694)
                     /gene="secB"
                     /locus_tag="ACHJWA_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFO4309822.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12757..13008)
                     /gene="grxC"
                     /locus_tag="ACHJWA_003284"
     CDS             complement(12757..13008)
                     /gene="grxC"
                     /locus_tag="ACHJWA_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFO4309823.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13150..13581)
                     /gene="yibN"
                     /locus_tag="ACHJWA_003285"
     CDS             complement(13150..13581)
                     /gene="yibN"
                     /locus_tag="ACHJWA_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFO4309824.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13826..15370
                     /gene="gpmM"
                     /locus_tag="ACHJWA_003286"
                     /gene_synonym="pgmI"
     CDS             13826..15370
                     /gene="gpmM"
                     /locus_tag="ACHJWA_003286"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFO4309825.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15404..16663
                     /gene="envC"
                     /locus_tag="ACHJWA_003287"
     CDS             15404..16663
                     /gene="envC"
                     /locus_tag="ACHJWA_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFO4309826.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16667..17626
                     /gene="yibQ"
                     /locus_tag="ACHJWA_003288"
     CDS             16667..17626
                     /gene="yibQ"
                     /locus_tag="ACHJWA_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFO4309827.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17613..18648)
                     /gene="waaH"
                     /locus_tag="ACHJWA_003289"
                     /pseudo
     CDS             complement(17613..18648)
                     /gene="waaH"
                     /locus_tag="ACHJWA_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
     gene            complement(18887..19912)
                     /gene="tdh"
                     /locus_tag="ACHJWA_003290"
     CDS             complement(18887..19912)
                     /gene="tdh"
                     /locus_tag="ACHJWA_003290"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFO4309828.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMCSGQSG
                     KVILSWD"
     gene            complement(19922..21118)
                     /gene="kbl"
                     /locus_tag="ACHJWA_003291"
     CDS             complement(19922..21118)
                     /gene="kbl"
                     /locus_tag="ACHJWA_003291"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFO4309829.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANVRQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(21393..>21588)
                     /locus_tag="ACHJWA_003292"
     CDS             complement(21393..>21588)
                     /locus_tag="ACHJWA_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="WlaTC/HtrL family glycosyltransferase"
                     /protein_id="HFO4309830.1"
                     /translation="QKITLNNNIVDDDQGIFVMCYYKRPDLFNLNYLGRGKWFDLFRC
                     FRSNTLGAKMQALRIFLSRK"
BASE COUNT         5470 a         5604 c         5512 g         5002 t
ORIGIN      
        1 aatatttaat cgacacacat cactgtcatt aatgtaaagc aacatagatc accaataata
       61 caatggacaa ctctaccggc attgatcgct gggcggagga aataaggatt tttacatgaa
      121 ctagaagaag atttttttca gtgctgtcac tgtttcagtt ctctgcgcat taacgggatg
      181 tgactacatt gaagaaggca agccagaaag tagtttattg aaacaacaag aagaacataa
      241 caataaaatt gatctacttg aaaaacaaca agcacagcta aagagtcaac tggaaactat
      301 tcaaaaacaa caaactggca taataagcag tacaaagact ttaactcacg taattaaatc
      361 cgttaaagat caacaaaaca cttttatttt taccgagttt aacccagcaa aaacgaagta
      421 tttcatcctg aacaatggtt cggtggcttt agcgggtcga gtgttatcca ttgacgccac
      481 agagaatggc agtgttattc atatttcact ggtcaactta ttaagtacac ctatctcaaa
      541 tattggtttt aatgcgacat ggggtggtga aaaacctgtc gatgccaaag agtttgcccg
      601 gtggcaacaa ttacttttca acacatcaat gaaatccaca ttgaaattat taccaggtca
      661 atggcaagac attaatttga ccctgaaggg tgtatcgccc aataacctgg gatatctgaa
      721 gttagccatc aacatggaaa atattcagtt cgacaatctt ccctctgctg aaaatcggca
      781 gaaaagaagc aaaaaataaa tgcagttagt acgcttataa caaggaatag cgagcatcat
      841 taatgaacaa aatatttaaa gttatctgga accctgcgac agggaattat actgttacca
      901 gcgaaacggc aaaaagccgt ggcaagaaat ctgggcgcag taagctgtta atttctgcgc
      961 tggttgcggg tggaatgttg tcgtcgtttg gggcattggc gaatgccggg aatgacaacg
     1021 gtcagggtgt tgattacggt agtggatcag ctggcgacgg ctgggttgct ataggcaaag
     1081 gggcgaaagc aaatactttt atgaacacca gtggttccag tactgctgtg ggttatgacg
     1141 ctatagctga aggccaatat agctctgcca tcgggtcaaa aacccatgcg attggtggtg
     1201 catcaatggc ctttggggtt agtgcaatat cagaaggcga tagaagtata gcactgggtg
     1261 cctcttcgta ttcattgggc caatactcaa tggccctcgg ccgttattca aaagcattgg
     1321 gtaaattgtc tattgctatg ggggactctt ccaaagcgga aggagcaaac gccattgccc
     1381 tgggaaatgc cactaaagct actgagatta tgagtattgc tcttggcgac accgccaatg
     1441 cgtcaaaagc gtattcaatg gcgctgggag caagtagcgt cgcatctgaa gaaaacgcta
     1501 ttgcgatagg tgctgagacc gaagccgctg aaaatgcaac tgctattggc aataatgcga
     1561 aggcaaaagg gactaatagc atggcaatgg ggttcggaag ccttgccgat aaagtcaata
     1621 ctatcgcatt aggaaatggc agccaggctc tggcagataa tgcaatcgcc ataggccagg
     1681 gcaacaaagc tgatggcgtg gatgccatcg ctctgggtaa tggtagccag tcgagaggct
     1741 taaacaccat tgccttaggc acagccagta atgcaactgg tgataagagt cttgcgcttg
     1801 gtagtaatag cagtgccaac ggtattaact ctgtcgcgct gggcgcagat tccattgcgg
     1861 atttagacaa taccgtctct gtcggcaata gttcattaaa acgcaagatc gttaatgtga
     1921 aaaatggcgc gatcaagtct gacagttacg atgccattaa tggttcacag ctttatgcca
     1981 ttagcgactc ggtagcaaaa aggcttggag gaggggctgc agtagatgtt gatgacggta
     2041 ctgttacagc accaacctac aatttaaaaa atggtagcaa aaataacgta ggggctgcgc
     2101 tcgctgtact tgatgaaaac accctgcaat gggaccaaac caaaggcaaa tacagcgctg
     2161 ctcatggtac tagtagccca actgccagcg taatcaccga tgttgcggat ggcacgattt
     2221 cagcctccag taaggatgcg gttaacggtt cccaactgaa agctaccaat gacgatgtcg
     2281 aagccaacac cgccaatatc gctactaata ccagcaacat tgccacgaat acggcaaata
     2341 ttgccaccaa taccaccaat atcaccaacc tgacggattc cgttggtgac cttcaggctg
     2401 atgccctgct ctggaacgaa actaaaaagg cattcagtgc agctcacggc caggatacca
     2461 ccagcaaaat caccaacgtt aaagatgccg acctgacggc tgacagcact gatgctgtta
     2521 acggctctca gctgaaaacc accaacgatg ctgtggcgac gaataccacc aatatcgcca
     2581 ataacacttc caatattgcc actaacacca ccaacatctc taacctgact gagacggtga
     2641 ctaatcttgg tgaggatgcg ctgaaatggg ataaggacaa tggtgtattc acggcagctc
     2701 atggcaccga gaccaccagc aaaatcacca acgttaaaga tggcgacctg acgactggca
     2761 gcaccgatgc cgttaacggc tctcagctga aaaccaccaa cgatgccgtg gcgacgaata
     2821 ccaccaatat cgccactaac accaccaaca tctctaatct gactgagacg gtgactaatc
     2881 ttggtgagga tgcgctgaaa tgggataagg acaatggtgt cttcactgca gctcatggca
     2941 acaataccgc cagcaaaatc accaatatcc tggacggcac agtcactgca accagttccg
     3001 atgccattaa cggtagccag ctttatgact taagcagcaa tatcgccacc tacttcggcg
     3061 gcaatgcttc tgtgaatact gacggtgtgt ttaccggtcc aacctacaaa atcggtgaaa
     3121 caaattatta taacgtcggc gatgcactgg ctgcgattaa ctcctcattt agcacgtctc
     3181 tcggcgatgc tctgctttgg gatgccaccg caggtaaatt cagtgccaaa cacggtacta
     3241 atggtgacgc aagcgtgatc actgatgtcg cagatggtga aatttcagac tccagttctg
     3301 acgcagtaaa cggctcacaa ctccacggcg tgagcagtta tgttgttgat gcgctggggg
     3361 gtggtgccga agtcaatgca gacggcacca tcactgcgcc gacgtacacc attgctaatg
     3421 ctgattacga taatgtcggt gatgccctga atgctatcga taccactctt gacgacgctc
     3481 tgctctggga tgcggacgcc ggtgaaaatg gtgcatttag cgccgctcac ggaaaagata
     3541 aaactgccag tgtaatcact aacgtcgcta acggtgcaat ctctgctgcc agcagcgacg
     3601 cgattaacgg ctcacaactc tataccacca ataagtacat cgctgatgcg ctgggtggtg
     3661 acgcagaagt caacgctgac ggcaccatca ccgcaccgac ttacaccatt gcgaacgccg
     3721 agtacaacaa cgtcggtgac gccctggatg cgcttgatga taacgccctg ctgtgggatg
     3781 agactgccaa tggcggtgct ggagcctaca atgccagcca tgacggtaaa gccagcatca
     3841 tcactaatgt cgctaatggc agtattagtg aggacagtac cgatgcagtg aacggttctc
     3901 agttgaatgc gacgaatatg atgattgagc agaacaccca aattatcaat cagctcgctg
     3961 gtaacaccga cgcaacctat atccaagaaa acggtgcggg tattaactat gtgcgtacta
     4021 acgacgacgg cttagcgttc aacgacgcca gcgcacaggg tgttggcgct acagctatag
     4081 gttataactc tgtcgccaaa ggcgatagca gcgtagctat tggtcagggc agctacagcg
     4141 acgttgatac gggtatcgcc ctgggtagca gctctgtttc cagccgagtg attgccaaag
     4201 gctcccgtga caccagcata acggaaaatg gcgttgttat tggttacgac accacggatg
     4261 gcgaactgct cggtgcattg tctatcggtg atgacggtaa atatcgtcaa atcatcaacg
     4321 tagccgatgg ttccgaagcc catgacgccg ttacggttcg tcaattgcag aatgcgattg
     4381 gtgcggtcgc aaccacgccg actaaatact tccacgctaa ttcaacggaa gaagattcac
     4441 tggcagtggg aactgactcg ctggcaatgg gtgcgaaaac catcgtgaat ggcgataaag
     4501 gtattggtat cggttatggt gcctacgtgg acgcgaatgc acttaacggc attgccattg
     4561 gtagcaatgc gcaagtcatt catgtcaaca gtattgcgat aggtaatggt tctacgacca
     4621 ctcgtggcgc tcaaaccaat tataccgcct acaacatgga cgcaccgcag aactctgtcg
     4681 gtgaattctc agtcggtagt gcggatggtc aacgtcagat cactaacgtc gcagcaggtt
     4741 cggctgatac cgatgcggtc aacgtgggtc agttgaaagt aacggatgcg caggtttccc
     4801 agaataccca gagcattact aacctggata atcgggtaac gaatcttgat tcacgcgtca
     4861 ccaatatcga aaacggtatt ggcgatatcg tcaccaccgg tagcaccaag tacttcaaga
     4921 ccaataccga tggtgtagat gccagcgcgc agggtaaaga tagcgtcgcg attggttccg
     4981 gctccattgc tgccgctgac aacagcgtcg ctctgggtac agggtctgtg gcaaccgaag
     5041 aaaatacgat ctctgtaggt tcctctacta accaacgtcg tatcaccaac gtagctgcag
     5101 gtaaaaatgc taccgatgct gttaacgtgg cacagttgaa gtcttccgaa gctggcggtg
     5161 tacgttacga caccaaagct gatggttcta tcgactatag caatatcacc ctcggtggcg
     5221 gcaacggcgg tacgactcgt atcagcaacg tctccgctgg cgtcaacaac aacgacgtgg
     5281 tgaattacgc gcagttgaag caaagcgtgc aggaaacgaa gcaatacacc gatcagcgaa
     5341 tggttgagat ggataacaaa ctgtctaaaa ctgaaagcaa gttgagcggt ggtatcgctt
     5401 ctgcaatggc aatgaccggt ctgccgcagg cttacactcc aggtgccagc atggcctcta
     5461 ttggtggcgg tacttacaac ggtgaatcgg cagttgcttt aggtgtatcg atggtgagcg
     5521 ccaatggtcg ttgggtctac aaattacaag gtagtaccaa tagccagggt gaatactccg
     5581 ccgcactcgg tgccggtatt cagtggtaat gatccattaa gttagtgtga ctaagggcaa
     5641 acgccaaatt gcctgatgcg ttacgcgcat caggcaagat aaggcgctcc gggtcaacaa
     5701 tctaagggca attctctgat gaggattgcc cttttcttta ccagacatct ccccccacaa
     5761 gaattggccc taccaattct tcgcttatct gacctctggt tcacaattcc ccaattaaaa
     5821 ctcacatcaa tattgccaat acataacatt tagttaacca ttcattgtca ttatccctac
     5881 acaacacaat tggcagtgcc acttttacac aacgtgtgac aaggagatga gcaacagact
     5941 cattacacga tgtgcgtgga ctccaggaga cctgcaatga atctctggca acaaaactac
     6001 gatcccgccg ggaatatctg gctttccagt ttgatagcat cgcttcccat cctgtttttc
     6061 ttctttgcgc tgattaagct caaactgaaa ggatacgtcg ccgcctcatg gacggtagca
     6121 atcgcccttg ccgtggcttt gctgttctat aaaatgccgg tcgctaacgc gctggcctcg
     6181 gtggtttatg gcttcttcta tggtttgtgg cccatcgcgt ggatcattat tgcagcagtg
     6241 ttcgtctata agatctcggt gaaaaccggg cagtttgaca tcattcgctc gtctattctt
     6301 tcgataaccc ctgaccagcg tctgcaaatg ctgatcgtcg gtttctgttt cggcgctttc
     6361 cttgaaggag ccgcaggctt tggcgcaccg gtagcaatta ccgccgcatt gctggtcggc
     6421 ctgggtttta aaccgctgta cgccgccgga ctgtgcctga ttgttaacac cgcgccagtg
     6481 gcatttggcg cgatgggcat tccaatcctg gttgccggac aagtaacagg catcgacagc
     6541 tttgagattg gccagatggt ggggcggcag ctaccgttta tgaccattat cgtgctgttc
     6601 tggatcatgg cgattatgga cggctggcgc ggtatcaagg agacgtggcc tgcggtcgtg
     6661 gttgcgggcg gctcgtttgc catcgctcag taccttagct ctaacttcat tgggccggag
     6721 ctgccggaca ttatctcttc gctggtatca ctgctctgcc tgacgctgtt cctcaaacgc
     6781 tggcagccag tgcgcgtatt ccgttttggt gatttggggg cgtcacaggt tgatatgacg
     6841 ctagcccaca ccggttacac tgcgggtcag gtgttacgtg cctggacacc gttcctgttc
     6901 ctgacagcta ccgtaacact gtggagtatc ccgccgttta aagccctgtt cgcatcgggt
     6961 ggcgcgctgt atgagtgggt gatcaacatt ccggtgccgt acctcgataa actggttgcc
     7021 cgtatgccgc cagtggtcag cgaggctaca gcctatgccg ccgtgtttaa gtttgactgg
     7081 ttctctgcca ccggcaccgc cattctgttt gctgcactgc tctcgattgt ctggctgaag
     7141 atgaaaccgt ctgacgctat cagcaccttc ggcagcacgc tgaaagaact ggctctaccc
     7201 atctactcca tcggcatggt gctggcgttc gcctttatct cgaactattc cggactgtca
     7261 tcaacactgg cactggcact ggcgcacacc ggtcatgcat tcaccttctt ctcgccgttc
     7321 ctcggctggc tgggggtatt cctgaccggg tcggatacct catctaacgc cctgttcgcc
     7381 gcgctgcaag ccaccgcagc acaacaaatt ggcgtctctg atctgttgct ggttgccgcc
     7441 aataccaccg gtggcgtcac cggtaagatg atctccccgc aatctatcgc tatcgcctgt
     7501 gcggcggtag gcctggtggg caaagagtct gatttgttcc gctttactgt caaacacagc
     7561 ctgatcttca cctgtatggt gggcgtgatc accacgcttc aggcttatgt cttaacgtgg
     7621 atgattcctt aatgattgtt ttacccagac gcctgtcaga cgaggttgcc gatcgtgtgc
     7681 gggcgctgat tgatgaaaaa aacctggaag cgggcatgaa gttgcccgct gagcgccaac
     7741 tggcgatgca actcggcgta tcacgtaatt cactacgcga ggcgctggca aaactggtga
     7801 gcgaaggcgt gctgctcagt cgacgcggcg gcgggacgtt tattcgctgg cgtcatgaca
     7861 catggtcgga gcaaaacatc gtccagccgc taaaaacgct gatggccgat gatccggatt
     7921 acagtttcga tattctggaa gcccgctacg ccattgaagc cagcaccgca tggcatgcgg
     7981 caatgcgcgc cacacctggc gacaaagaaa agattcagct ttgctttgaa gcaacgctaa
     8041 gtgaagaccc ggatatcgcc tcacaagcgg acgttcgttt tcatctggcg attgccgaag
     8101 cctcacataa catcgtgctg ctgcaaacca tacgcggttt cttcgatgtc ctgcaatcct
     8161 cagtgaagca tagccgtcag cggatgtatc tggtgccacc ggttttttca caactgaccg
     8221 aacaacatca ggctgtcatt gacgccattt ttgctggtga tgccgacggg gcgcgtaaag
     8281 caatgatggc gcaccttagt tttgttcaca ccaccatgaa acgattcgat gaagatcagg
     8341 ctcgccacgc acggattacc cgcctgcccg gtgagcataa tgagcattcg agggagaaaa
     8401 acgcatgatt atttccgcag ccagcgatta tcgcgccgca gcgcaacgca ttctgccgcc
     8461 gttcctgttc cactatatgg atggtggtgc atattctgaa tacacgctgc gccgcaacgt
     8521 ggaagatttg tcagaagtgg cactgcgcca gcgtattctg aaaaacatgt ctgacttaag
     8581 cctggaaacg acgctgttta atgagaaatt gtcgatgccg gtagcactgg caccggtggg
     8641 tttgtgtggc atgtatgcgc gtcgtggcga agttcaggca gccaaagcgg cggacgcgca
     8701 tggcattccg tttactctct caacggtttc cgtttgcccg attgaagaag tcgcgccagc
     8761 catcaagcgc ccaatgtggt tccagcttta tgtactgcgc gatcgcggct ttatgcgtaa
     8821 cgcgctggag cgagcaaaag cagcgggttg ttcgacgctg gttttcaccg tggatatgcc
     8881 gacaccggga gcgcgttatc gtgatgcgca ttctgggatg agcggcccaa atgcggcaat
     8941 gcgccgctac ttgcaagcgg tgacgcatcc gcaatgggcg tgggatgtgg gcctgaacgg
     9001 tcgtccgcat gatttaggta atatttcggc ttacctcggc aaaccgaccg gactggaaga
     9061 ttacatcggc tggctgggga ataacttcga tccgtccatc tcatggaaag accttgagtg
     9121 gatccgcgat ttctgggatg gcccgatggt gatcaaaggg atcctcgatc cggaagatgc
     9181 gcgcgatgca gtacgttttg gtgctgatgg gattgtagtt tctaaccacg gtggccgcca
     9241 gctggacggt gtactctctt ccgctcgtgc gttgcccgct attgcagatg cggtgaaagg
     9301 tgatatcgcc attctggcgg atagcggaat tcgtaacggg cttgatgtcg tgcgtatgat
     9361 tgcgctcggt gccgacaccg tactgctggg tcgtgctttc ctgtatgcgc tggcaacagc
     9421 gggccaggcg ggtgtagcta acctgctaaa tctgatcgag aaagagatga aagtggcaat
     9481 gacgctgact ggcgcgaaat cgatcagtga aattacgcaa gattcgctgg tgcaggggct
     9541 gggtaaagaa ttgcctacgg cactggctcc gatggcgaaa gggaatgcgg catagtcgtt
     9601 tgagccccct caccctaacc ctctccctca gggagtgggg accgttcggc gctgtatgta
     9661 ctccctcact ctgaaacgac accgcactct ttttttctcc ctcgcccctc cggggtgagg
     9721 ggaaaaggcc gcactgctca ccaattttct gctatcctgc ccccgcacta agggggcagt
     9781 atgctaaaca tcgtacttta cgaaccagaa attccgccaa atactggcaa tatcatccgt
     9841 ctttgcgcta ataccggctt tcgtctgcat atcatcgaac cgatgggatt tgcctgggac
     9901 gataagcgcc tgcgccgcgc ggggctggac tatcacgagt ttaccgccgt tacgcgtcat
     9961 catgactttc gcgcgttcct cgaagcagaa aatccccagc gcctgtttgc cctcaccacg
    10021 aaaggtacgc ctgctcacag cgccgtaaac tatcaggatg gcgattatct gatgttcggc
    10081 ccggaaacac gcggcctgcc agcgagcatt cttgatgccc tgcccgctga acaaaaaatt
    10141 cgcattccga tggtgccgga cagccgcagc atgaacctgt ccaatgcggt gtcagttgtg
    10201 gtgtatgaag cctggcggca gctgggatat ccgggagcgt tgttgagaga ttagttactg
    10261 tatgccggat agatgattac atcgcatccg gcacgatcac aggacattag atcccatccc
    10321 catactcaaa tgtatggtta ataccgttga aatgctggtc catatccatt gatggcttat
    10381 cgctgtctgg tttaccgaca atacgagccg gaacgccagc ggcggttgta tgcggcggca
    10441 ccggttgcag caccacggaa cctgcgccaa tcttcgcgcc gcgaccaact tcaatattgc
    10501 cgaggatttt cgcgcccgcg ccaatcatca caccttcacg aattttcggg tgacggtcac
    10561 caccagattt acccgtaccg ccaagcgtca cagattgcag aatcgatacg tcgttttcaa
    10621 ttaccgccgt ttcacccaca acgatgcccg tggcgtggtc gagcataatg ccacggccaa
    10681 tttttgctgc cgggtgaata tcgacctgga acgtcacaga aacctggttt tgcagaaaga
    10741 ttgccaatgc gcgacgcccc tgattccaca accagtgacc gatgcgatag gcctgcaagg
    10801 catgaaaacc cttcaggtat aacaacgggg ttgagtattt atcgactgcc gggtcgcggg
    10861 tacgcaccgc ctgaatatca caggccgcag aggcgatcat ttccgggtca gcggcgtagg
    10921 cttcttccac cacttcacgg atagcaatag caggcataat tggcgatgac agcttgttcg
    10981 ccagcatgta gctcagtgca ctaccaaggt tttcgtgctt gagtagcgtc gcgtggtaaa
    11041 aactggccag cattggctca cagtccgcca gcgttctggc ttcggcttta atattgttcc
    11101 agacaatttc cagttcttca cacgacattg cttactccac acgatgagat aatgaccggc
    11161 ccgttctgcg cgggccgggt catagcggta acaaaggttc cctggggtta gtggctgctg
    11221 cgctcgtcct tgcgtgcacg acctaataac gtcaatgctg cctcgcgcgc gttttttccg
    11281 caatataata cttgataaat ttcctcggtt attggcattt ctacgccgaa gcgatgcgcc
    11341 agttcgcgga cttctttcgt attgcggtag ccttccacca cctgaccaat cttctcctgc
    11401 gcgctttgta catccatgcc ctgaccgagc atcatgccaa aacggcggtt acgcgactgg
    11461 ttgtcggtac aagtaagcac aagatcgccc agccccgcca tgcccataaa ggtggcagga
    11521 tcggcaccca gcgccgcgcc aagacgcgac atttcagcca gcccacgggt gatcagtgcc
    11581 gtacgcgcat tcgcaccaaa accgataccg tcggacatcc ccgcgccaat ggcaatgacg
    11641 tttttcaccg caccgccaag ctgcacgcca atgaaatccg gattgctgta aacgcggaaa
    11701 cttttgccgc agtgcagcag ctgctggaga tcatcggcaa aggtctgatc ggtcgaggcc
    11761 agcgaaatag ctgtcggtaa acctgccgcc agttctttcg caaacgttgg gccagagata
    11821 accgccagcg gaatttgatc gcctaaggcc tcacgcgcca cgtcctgtaa cagacgtccg
    11881 gtttccgctt ccagcccttt ggtcgcccac accagacgcg catcaggacg catcagcggt
    11941 ttaatctggc gcagcacttc accaaagaca tggctgggta cgacgacgag aatattacgg
    12001 ctggctgcca gcgcagtggc gagatcgctt tcaagatgga gcgtatcggg aaaaggcaca
    12061 tcggggagaa acgcggcgtt acagcggtcg cgttcaagcg ttgcgatatg ttcagggtca
    12121 tggccccaga ggacaacctc gtggccattt cttgccaggg tgatggcaag agcggtgccg
    12181 tacgagccgg caccgatcac agtcattgaa gcattacgtt ggttcatcag gcatcctgat
    12241 gttcttcagt accttcgcca gcctgctgct gcaaatagtt catgaacagc gcatcgaagt
    12301 taaccggcgc aaggttcagt tgcgggaatg taccgcggga taccatgctg gtgatgcact
    12361 cacgagcata cgggaacaga atgttcgggc agtatgcgcc caggcaatgt gccatctggg
    12421 tgccttcgat acccgcgatg gagaaaatac cgccctgctg aacttcacac aggaacgcgg
    12481 tttcttcgcc cagggaggcc gttacggtaa cacgcagtac cacttcgtat acgtcatctg
    12541 ccagttggga agatgccgta tccagatcaa gtttaacttc tggttgccaa tctttctgga
    12601 aaacgtgcgg cgcgttcggc gcttcgaaag agatatcctt ggtataaata cgttggatct
    12661 ggaaagtcat ttcagtgttg ttttgttctg acatgtgtag aaaaccctta agtgttgtcc
    12721 ttaaatactg cgcaatgtaa tgccaacgtt cacacgttat ttcagcaggg gatccagtcc
    12781 accacgtgca tccaatgcat acaagtcatc acagccgcca atgtgctgtg cgtcaataaa
    12841 aatctgggga accgtggtgc gaccgctgcg tttgatcatc tcttcacgct tggcggcatt
    12901 gccatcgatc ggcaactcct gaaaactcac gcccttgctg ctcagcagtg cttttgcacg
    12961 atggcaatac gggcaggttt ctttggtata gatttcaaca ttggccataa tttagctcct
    13021 gtttttttac cctgaaattt catgttgcag ggcggctgca aaatggagtg tccccagaag
    13081 cttataacaa taagtcgctg ggggcctaat tgcagctaac ggcctgcatc atgaaagacg
    13141 acaggtaaat tatttgccgc gcaccaaagg caagttttcg ccagcccagc cagcgacgcc
    13201 ttctttcagc acgaatactt gcgcaaaacc agctttcgtc agtgcgtttg caggctcctg
    13261 gcactgcatg ccagaaccgt ctaccacgat aaccggtttg tctttgtgct tctcaagctc
    13321 accaacattg ttggctttga tttcgctcgg caacagattg atagaacctg cgatatggcc
    13381 tttacggaag tcatcacgct gacgtaaatc cacaaccaca gcgtcttctt tgttgatcag
    13441 acgcgtagct tcaccacgag taatcacctt cactttcgag gtcaggctct taaacgtagt
    13501 cacaagaacc gccaccagta acgcgatcca ggcgatactc agtatgggat gacggccaac
    13561 aaattgcata atttcttgca tggggggtaa caactcccga cgtagtgatt aaaaaaacca
    13621 ggaaaggagt atacctgcgc ggtgcggcaa atacagccag cgcgttaact ggaatgcaat
    13681 tttgcggggc acgacgaaaa aaaagcgcaa atctggcatc ggcttgccgc gagcggtcgt
    13741 atattttgat cttcagaggc tattttatcg attcagctgt agtaaaatta cgcaaatttt
    13801 gactcttgag tatgaggttg tcgcaatgtc ggtttctaaa aaacctatgg tactggtgat
    13861 tctggatggc tatggctatc gtgaagaaca gcaggataac gccattttta gtgctaaaac
    13921 cccggtaatg gatgcactgt gggccaatcg tccgcatacc ctgatcgacg cttccggtct
    13981 ggaagtcggt ctgcctgacc gtcagatggg taactccgaa gtaggccacg ttaacctggg
    14041 tgccggccgc atcgtgtatc aggacctgac tcgtctggac gttgaaatca aagatcgcgc
    14101 tttctttgct aatccagtgc tgactggcgc ggtagataaa gcgaaaaacg caggtaaagc
    14161 agtacacatt atgggtctgc tctccgcagg cggcgtacac agccacgaag atcacatcat
    14221 ggcgatggta gaactggcag ctgaacgcgg cgcagaaaaa atctacctgc acgcattcct
    14281 tgacggtcgc gacaccccgc cgcgcagtgc tgaatcctcg ctgaaaaaat ttgaagagaa
    14341 atttgctgcg ctgggtaaag gccgcgtagc gtccatcatt ggtcgttact acgcgatgga
    14401 ccgcgataac cgttgggatc gcgttgaaaa agcctatgac ctgctgactc tggcgcaggg
    14461 tgagttccag gccgatactg ccgttgctgg tttgcaggca gcttatgctc gcgacgaaaa
    14521 cgacgagttc gtgaaagcga ccgttattcg tgcagaaggt cagccagatg cggcaatgga
    14581 agacggcgat gcgctgattt tcatgaactt ccgtgctgac cgcgcgcgcg aaatcactcg
    14641 tgcattcgta aacgcagatt tcgacggctt cgcgcgtaag aaagtggtta acgtcgattt
    14701 cgtgatgctg accgaatacg ccgctgacat caaaactgcg gttgcttacc cacccgcttc
    14761 cctggttaac accttcggcg agtggatggc gaaaaacgac aaaactcagt tgcgtatttc
    14821 cgaaaccgaa aaatatgccc acgttacttt cttcttcaac ggtggcgtag aagagtcgtt
    14881 caaaggcgaa gatcgcattc tgatcaactc accgaaagtg gctacctacg atctgcaacc
    14941 ggaaatgagc tccgcagagc tgaccgaaaa actggttgcg gccatcaaga gcggcaaata
    15001 cgacaccatt atctgtaact atccgaacgg cgacatggta ggtcacaccg gggtgatgga
    15061 agcggcggtt aaagcggttg aagcactgga tcactgcgtg gaagaagtcg cgaaagcggt
    15121 tgagtccgtg ggtggacaac tgctgatcac cgctgaccac ggtaacgctg aacagatgcg
    15181 cgatccggca acgggtcagg cacacacggc acacaccaac ctgccagttc cgctgattta
    15241 cgttggtgat aagaacgtga aagcggttgc aggcggcaaa ctttctgaca tcgcgccgac
    15301 catgttgtcg ctgatgggta tggaaatccc gcaagagatg actggtaagc cgctgttcat
    15361 cgtggaataa tccctcccca tgaggggaaa ggcgattaat accatgacac gggccgtgaa
    15421 accgcgcagg tttgcaatca ggcccatcat ctacgccagc gttcttagcg ctggcgtatt
    15481 gttgtgcgcc ttttccgccc acgcggatga gcgtgaccaa ctcaaatcta ttcaggccga
    15541 tatcgccgca aaagagcgcg cggtacgcca aaagcaacaa caacgcgcaa gcctgctcgc
    15601 acaattgaaa aagcaggaag aagcgatctc tgaagccacc cgtaagctgc gcgaaacgca
    15661 aaacacgctc aatcaactca ataaacagat tgatgagatg aacgcgtcga ttgccaaact
    15721 ggagcagcaa aaagccgccc aggagcgcag cctcgccgca caactggatg ccgcattccg
    15781 tcagggcgag cataccggta ttcagctgat tctcagcggt gaagaaagcc agcgtggaca
    15841 gcgtttacag gcttatttcg gctatctcaa ccaggcgcga caagaaacca ttgcccagtt
    15901 gaagcaaacg cgtgaagaag tcgccatgca gcgtgctgaa ctggaagaga aacagagcga
    15961 gcaacaaacg ctgttatatg agcagcgcgc ccaacaggcg aaactgactc aggcgctgaa
    16021 cgagcgtaaa aagacgctgg cagggctgga gtcttccatc cagcaaggtc agcaacagtt
    16081 gagcgagctg cgcgccaacg aatcccgtct gcgtaacagc attgcccgtg cggaagccgc
    16141 ggcgaaagcg cgtgcagaac gagaagcacg tgaggcccag gcggttcgcg accgccagaa
    16201 agaagcgacg cgcaaaggca ccacctacaa accgaccgaa agcgaaaaat cgctgatgtc
    16261 ccgaactggt ggcctggggg cgccgcgtgg tcaggcattc tggccggttc gcgggccgac
    16321 gctgcatcgc tatggtgaac agctacaggg cgaactacgc tggaaaggaa tggttatcgg
    16381 tgcctctgaa ggtactgaag ttaaagcgat tgccgatggt cgggtgattc tggctgactg
    16441 gctgcaaggt tacggtctgg tggtggtggt tgagcacggt aaaggcgaca tgagtcttta
    16501 cggctataat cagagcgcac tggtgagcgt tggttcgcag gttcgcgcgg gccagccaat
    16561 tgcactggtg ggcagcagtg gcggtcaggg tcggccttca ctctatttcg aaattcgccg
    16621 ccaaggtcag gcggtcaatc cacagccgtg gttgggaaga taagttttgt ttccatttcg
    16681 tcgtaacgtt cttgcatttg ccgctctgtt ggcgctctcc tcccccgtac ttgctggcaa
    16741 acttgccatc gtcattgatg attttgggta tcgcccgcac aacgaaaacc aggtgctggc
    16801 gatgccctcc gctatctccg tcgctgtatt acccgattca ccgcacgcca gagagatggc
    16861 gaccaaagcg cataacagcg ggcacgaagt gttgattcat ctcccgatgg caccactgag
    16921 taaacagccg ctggagaaaa atacgctgcg cccggagatg agcagcgacg aaattgagcg
    16981 cattattcgt agtgcggtca ataacgtgcc ctatgccgtg gggatcaaca accacatggg
    17041 tagcaagatg acctctaacc tgtttggtat gcagaaagtg atgcaggcac tggagcgtta
    17101 caatctttac ttcctcgaca gcgtaaccat cggtaatacc caggcgatgc gcgccgcgca
    17161 aggtactggc gtgaaggtga tcaaacggaa ggtgttcctc gacgattcgc agaacgaagc
    17221 ggacatccgt gtgcaattta atcgcgcaat tgatctggcg cgtcgcaacg gttcaaccat
    17281 tgccattggg catcctcacc cttcaacggt acgcgtgcta caacagatgg tttataacct
    17341 gccgccagac attacgctgg tgaaagccag cagcttgctg aatgaaccgc aggttgatac
    17401 ttctacaccg ccgaaaaacg ctgtgcctga tgcaccgcgt aatccgttcc gtggcgtgaa
    17461 gctgtgcaaa ccgaagaaac cgatagaacc tgtttatgct aatcgcttct ttgaagtatt
    17521 aagcgaaagc atcagccaga gcacgctgat cgtttacttc cagcatcagt ggcaaggctg
    17581 gggcaaacag cccgaagcgg cgaagtttaa cgctagcgca aattaaaggc gctacgcgta
    17641 atgcgatggt gcgaccgcgt ttttttcgcg ccattgccat aagcggaaag accacagtaa
    17701 cgcctggtaa ccgaccttca cgctgcgcac attggtaatc aggcgcttat acataccgga
    17761 agtaaatatc tcggcaatca tccgttggcg ggtaagaata tccggctctt tgcgcaccgc
    17821 atggcaaaca cgcaatgctt cgtaagttat ttgctgatga aattccggat aaatcataat
    17881 tttgtcggca taatttcgat ttaatttctc cagcaggcgg gtaatcttaa tatagtgacg
    17941 ttgataatta aggtttttat tcccttgtct atgcaaccga ctcactgacg tattatgcag
    18001 ataatattta tataatgatt gctcggtata tcgcgcacgc agcgcgttaa acatgaattc
    18061 tgttgtccag acaatatcct gatgatgtaa tccggcaata aatttaatgt tatttttaac
    18121 aataacatca cggcgataaa cccccatcca gacaacgtga gtccaacggc gcgaagaaag
    18181 ccccatccgc agccagtccg ggccggttaa tacaccggtt gagcgaaggc gatcgctggg
    18241 gatggattgc caggtttctc ccgtttcacg aaaagaccag tcagcgttgc actgcgccac
    18301 gtcgaggtcg tcctctaacg ccatggtcat cagcgtttcg tacatggtgg gatagacctc
    18361 atcgtcagca tcgacaaaag cgacatattt gcccgtcgcc acctcgatcc cacgattacg
    18421 cgctaccgat gcgccagcat tcgcctgatg caacaaacga acgtgcggat agttttctgc
    18481 gtaatgcttt gctatttcaa cagaattatc cgttgaacca tcgttaataa taatgatttc
    18541 cagagcagtc caggtttgcg taattaaaga ttccatacaa gtgcggaaat catcgcccgc
    18601 attatataac ggaataataa cactaagttt attggtgctg ttcatcataa attaccgacg
    18661 acctctgaag ttatcctgac gttttacagg ctttgtatta agaaactatt aagcctgtga
    18721 gatattattt ttacaggtga gagagtcact tcagggtaga gagataatga gaacagctat
    18781 ataaaagatc agcgcaatta cgaactcttt attacactga caggatttta catatttaac
    18841 agattatcct caggcaaaag ggctggaata ccagcccttt ttcgtgttaa tcccagctca
    18901 gaataacttt cccggactgg cccgaacaca tagcgtcaaa gcccttctgg aaatcatcga
    18961 tagagaaacg atgggtaatg atcggcgaga gatcgaggcc agactgaatc agcgccgcca
    19021 tcttgtacca ggtttcaaac atctcacgac cgtaaatgcc tttaatgaac aagcctttaa
    19081 agatcacttt ggtccagtcg atagacatgt cagacggtgg aatacccagc atcgcaatac
    19141 gaccgccgtg gttcatggta tcgagcatgg tacgaaacgc tggcggcgcg ccggacattt
    19201 ccagaccgac atcaaagcct tcggtcatgc ccagttcagc catcacatca ttaaggtttt
    19261 ctttcgcgac gttaaccgca cgggtgatac ccattttgcg cgccagctca aggcggtatt
    19321 cgttaacatc ggtgatcacc acattgcgtg caccaacgtg tttcgccacc gccgcagcca
    19381 taatacctat cggacctgca ccagaaacca gcacatcttc gcccaccaga tcgaacgaca
    19441 gcgcggtatg cacggcgtta ccgaagggat caaaaatgga agccaggtcg tcggagatat
    19501 tgtcggggat tttgaaagcg ttgaacgccg gaatcaccag atattcggcg aagcagcccg
    19561 gacggttaac accgacacca atggtgttgc ggcacagatg ggtacgaccg ccacgacagt
    19621 tgcgacagtg accgcaggtg atatggcctt cgccagaaac gcgatcgccg attttgaagc
    19681 ctttcacttc ctgaccaata cctaccactt caccgacata ttcatggcct acgaccatcg
    19741 gcaccgggat ggttttttgc gaccactcat cccagttata gatgtgaacg tcagtcccgc
    19801 agatggctgt tttacggatt ttaatcagca gatcgttatg cccgagttcc ggtacaggaa
    19861 cgtcggtcat ccagatgccc tcttccgctt tcagtttgga taacgctttc atctcacatc
    19921 ctcaggcgat aacgcccagt tgtttaccaa tacgcgtgaa tgcttctact gcacgcgtaa
    19981 tttgctcagg ggtatgcgcc gcagacatct gggtacgaat acgcgcctga cctttcggaa
    20041 cgaccggata gaagaaaccg gtgacgtaaa tgccctcttt ttgcagctca cgggcaaatt
    20101 tctgcgccac tactgcatcg ccaagcatga ccggaataat ggcatgatcg gctcccgcca
    20161 gggtaaagcc cgccgccgac atttgctcac ggaactggcg cacgttcgcc cacagacggt
    20221 cacgcagttc gctgcccgct tcgaccatct ccagtacttt gatggacgcg gcaacaatgg
    20281 ccggtgccag cgagttggag aacaggtacg gacgagaacg ctggcgcagc cactcaacca
    20341 cctctttgcg cgccgcggta taaccaccag aagccccgcc cagcgcttta ccaagcgtac
    20401 cggtgataat atcgacccgg cccatcacat cgcagtattc atgggaacca cgaccatttt
    20461 caccgacaaa accgaccgcg tgggagtcat ccaccatcac cagggcatcg tatttatctg
    20521 ccagatcgca aacgcctttc aggttggcaa tcacgccgtc cattgagaac acaccatcgg
    20581 tggcaatcag cacatgacgc gcaccggctt cacgcgcttc tttcagacgt gcttccagct
    20641 cctgcatatc gttgttggca tagcgatagc gtttagcttt gcacagacgc acaccatcaa
    20701 taatagacgc gtggttcagt gcgtcggaga taatggcgtc ttccgcaccc agcagcgttt
    20761 caaacagtcc accgttagca tcaaagcagg aagagtagag aatcgcatct tccatcccca
    20821 ggaatgccgc cagtttttgt tcaagctctt tatggctgtc ctgagtgccg caaataaaac
    20881 gcaccgaagc catgccgaaa ccgtgagaat ccattcccgc ctttgctgcc gcaatcagat
    20941 ccggatgatt cgccagcccg agatagttgt tggcacaaaa gttaatgacg tggcttccat
    21001 cagccacagt gatatctgct tgctgcgcag acgtaataat gcgctcttct ttaaacaacc
    21061 cttccgcccg tgcggtttcc agatcgttgg ttaactgctg ataaaattct ccacgcattg
    21121 cgattctcca gacagggcaa attccagcac atattaccca aacttatagg tcgcgacgag
    21181 atgacgcgtt aactcttctg caaaattcag gataaatgac gtgtaacttg atgtctctca
    21241 acacctacag atagtagaaa aactgtacag ttttggctgg gcataatgat gattgatttt
    21301 actgcattga tataaaaaga aaaaatgatg ccaaagcatc atttttccta tctcagttgg
    21361 tatcaaaacg ctacccacta atggagggaa acctattttc ttgaaaggaa aattctcagc
    21421 gcctgcattt ttgcccctaa agtgttactc ctgaagcagc gaaacaaatc gaaccatttt
    21481 cctctcccca gatagtttag gttgaagaga tcggggcgtt tgtaatagca catgacaaaa
    21541 atgccctggt catcatccac aatgttatta tttagtgtta ttttttga
//
LOCUS       DAZFAB010000078        21482 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZFAB010000078 DAZFAB010000000
VERSION     DAZFAB010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21482
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.078"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..162)
                     /locus_tag="ACHJWA_003293"
     CDS             complement(<1..162)
                     /locus_tag="ACHJWA_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5503 domain-containing protein"
                     /protein_id="HFO4309831.1"
                     /translation="MFPLNDLSLKTQSVQLNKITSNTESTIKQHELVSDDAIINELSS
                     ELVSCLGNDK"
     gene            346..486
                     /locus_tag="ACHJWA_003294"
                     /pseudo
     CDS             346..486
                     /locus_tag="ACHJWA_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003844207.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(511..825)
                     /gene="fliE"
                     /locus_tag="ACHJWA_003295"
     CDS             complement(511..825)
                     /gene="fliE"
                     /locus_tag="ACHJWA_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFO4309832.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            1040..2697
                     /gene="fliF"
                     /locus_tag="ACHJWA_003296"
                     /pseudo
     CDS             1040..2697
                     /gene="fliF"
                     /locus_tag="ACHJWA_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310704.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
     gene            2690..3685
                     /gene="fliG"
                     /locus_tag="ACHJWA_003297"
     CDS             2690..3685
                     /gene="fliG"
                     /locus_tag="ACHJWA_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFO4309833.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            3678..4364
                     /gene="fliH"
                     /locus_tag="ACHJWA_003298"
     CDS             3678..4364
                     /gene="fliH"
                     /locus_tag="ACHJWA_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFO4309834.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            4426..5799
                     /gene="fliI"
                     /locus_tag="ACHJWA_003299"
     CDS             4426..5799
                     /gene="fliI"
                     /locus_tag="ACHJWA_003299"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFO4309835.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            5818..6261
                     /gene="fliJ"
                     /locus_tag="ACHJWA_003300"
     CDS             5818..6261
                     /gene="fliJ"
                     /locus_tag="ACHJWA_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFO4309836.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            6258..7385
                     /gene="fliK"
                     /locus_tag="ACHJWA_003301"
     CDS             6258..7385
                     /gene="fliK"
                     /locus_tag="ACHJWA_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFO4309837.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLISDIVSDAQQANLLIPVDETPPVINDEQSTSTPLTTAQ
                     TMALAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPQVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSSHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            7490..7954
                     /gene="fliL"
                     /locus_tag="ACHJWA_003302"
     CDS             7490..7954
                     /gene="fliL"
                     /locus_tag="ACHJWA_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFO4309838.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            7959..8963
                     /gene="fliM"
                     /locus_tag="ACHJWA_003303"
     CDS             7959..8963
                     /gene="fliM"
                     /locus_tag="ACHJWA_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFO4309839.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            8960..9373
                     /gene="fliN"
                     /locus_tag="ACHJWA_003304"
     CDS             8960..9373
                     /gene="fliN"
                     /locus_tag="ACHJWA_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFO4309840.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            9376..9741
                     /gene="fliO"
                     /locus_tag="ACHJWA_003305"
     CDS             9376..9741
                     /gene="fliO"
                     /locus_tag="ACHJWA_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFO4309841.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            9741..10478
                     /gene="fliP"
                     /locus_tag="ACHJWA_003306"
     CDS             9741..10478
                     /gene="fliP"
                     /locus_tag="ACHJWA_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFO4309842.1"
                     /translation="MRRLLSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            10488..10757
                     /gene="fliQ"
                     /locus_tag="ACHJWA_003307"
     CDS             10488..10757
                     /gene="fliQ"
                     /locus_tag="ACHJWA_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFO4309843.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            10765..11550
                     /gene="fliR"
                     /locus_tag="ACHJWA_003308"
     CDS             10765..11550
                     /gene="fliR"
                     /locus_tag="ACHJWA_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFO4309844.1"
                     /translation="MLQVTSEQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            11840..12463
                     /gene="rcsA"
                     /locus_tag="ACHJWA_003309"
     CDS             11840..12463
                     /gene="rcsA"
                     /locus_tag="ACHJWA_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFO4309845.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKLIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(12507..12695)
                     /gene="dsrB"
                     /locus_tag="ACHJWA_003310"
     CDS             complement(12507..12695)
                     /gene="dsrB"
                     /locus_tag="ACHJWA_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFO4309846.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            12858..13085
                     /gene="yodD"
                     /locus_tag="ACHJWA_003311"
     CDS             12858..13085
                     /gene="yodD"
                     /locus_tag="ACHJWA_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFO4309847.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            13383..14198
                     /gene="yedP"
                     /locus_tag="ACHJWA_003312"
     CDS             13383..14198
                     /gene="yedP"
                     /locus_tag="ACHJWA_003312"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFO4309848.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKMLGLQGLPLIAENGAVIQLAEQWQEIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(14195..15889)
                     /gene="dgcQ"
                     /locus_tag="ACHJWA_003313"
     CDS             complement(14195..15889)
                     /gene="dgcQ"
                     /locus_tag="ACHJWA_003313"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFO4309849.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHIVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRDAV
                     TEFEQHRDEHAWQIELNRRRTLSVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(16060..16242)
                     /gene="yodC"
                     /locus_tag="ACHJWA_003314"
     CDS             complement(16060..16242)
                     /gene="yodC"
                     /locus_tag="ACHJWA_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFO4309850.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(16321..17238)
                     /gene="yedI"
                     /locus_tag="ACHJWA_003315"
     CDS             complement(16321..17238)
                     /gene="yedI"
                     /locus_tag="ACHJWA_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFO4309851.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQVH"
     gene            17411..18331
                     /gene="yedA"
                     /locus_tag="ACHJWA_003316"
     CDS             17411..18331
                     /gene="yedA"
                     /locus_tag="ACHJWA_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFO4309852.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(18320..18790)
                     /gene="vsr"
                     /locus_tag="ACHJWA_003317"
     CDS             complement(18320..18790)
                     /gene="vsr"
                     /locus_tag="ACHJWA_003317"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFO4309853.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLKDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(18771..20189)
                     /gene="dcm"
                     /locus_tag="ACHJWA_003318"
     CDS             complement(18771..20189)
                     /gene="dcm"
                     /locus_tag="ACHJWA_003318"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFO4309854.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(20256..20951)
                     /gene="yedJ"
                     /locus_tag="ACHJWA_003319"
     CDS             complement(20256..20951)
                     /gene="yedJ"
                     /locus_tag="ACHJWA_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HFO4309855.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQSSSILAAEETRRLLREEFVQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDAEDPLAQ
                     HRPLDDKRYALDHFQTKLLKLPQIMQTVRGKQLAQHNAHFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            complement(20991..21271)
                     /gene="drpB"
                     /locus_tag="ACHJWA_003320"
                     /pseudo
     CDS             complement(20991..21271)
                     /gene="drpB"
                     /locus_tag="ACHJWA_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
BASE COUNT         5201 a         5682 c         5509 g         5090 t
ORIGIN      
        1 cttatcattt ccaagacaac tgactaactc acttgataat tcatttatga ttgcatcatc
       61 agatactaac tcgtgttgtt ttatcgtaga ctcagtattc gatgtgattt tatttaattg
      121 aaccgactga gtttttaacg acagatcatt taatggaaac atttacactc ctgtttaata
      181 aattatattg cccagggcgc attctaaaac acaataaaat taccactatc aaaaatcgct
      241 catatccata gcccggaaca tgaataaaaa catcaacaaa gataaaatta tccagaggtt
      301 actgttgcgt ctgtggtcgt aacaacatcc gacgtggtga tgtgtcaaat gagacactct
      361 gatgttcagg gggtttatcg tgtatacgga tcgtggatgg cagaaaactt tcaggatcag
      421 gtatccattt caaaccagat aatgagtaag tttgccccat ccatgcccca cgcggtgaga
      481 tcggatgtca ttaacaacag gttacacaac ctatacctgc atgctcatta cttcctgata
      541 tgccgccacc agcttattac gcacctgaat ccccatttgc atagaaactg aggctttttg
      601 catatcggtc atcacatcgt ttaacgccac gccgggttca ccgagagtga atttttctgc
      661 ctgcgtgcgg gcggctgttt gtgtatcgct tatgcgatcg agcgcggcgt gcagctgccc
      721 ggcaaaacta atggtcggtt gcggcagtga ttcctgcgca cgcgcactca tcgccgtagc
      781 ctgtaactgg ctgataaccc cttcaatccc ctgtatcgct gacattttca tctcctggat
      841 aatttctggt agcaaagcct accagtaagt caataagaca aaggcgctaa atagcaacaa
      901 aaaaacgggt ttattggcgg atagaaaaaa acgaaagcac aaataatggg agcgtcaatt
      961 tttcgagttt gctgacccgg gagtgagtct tgttccactt tgccaataac gccgtccata
     1021 atcagccacg aggtgcgcga tgaatgcgac tgcagcccag acaaaatctc ttggtggctt
     1081 aatcgcctgc gtgcgaatcc gaaaattcca ttgattgttg ccggttccgc ggcagtggcg
     1141 gtcatggtcg cactgatcct gtgggcgaaa gcccccgact accgcacatt attcagcaat
     1201 ctttccgatc aggatggtgg cgcaattgtc agccaactga cgcaaatgaa tattccttac
     1261 cgcttcagcg aagccagcgg cgctattgaa gttccggcag ataaagttca cgagctgcgc
     1321 ctgcgcctgg cgcaacaagg tttgcccaaa ggcggcgcgg tcggtttcga actactcgat
     1381 caggaaaagt ttggtatcag ccagttcagc gaacaggtga attatcagcg ggcgctggaa
     1441 ggcgagcttt ctcgtaccat cgaaactatc ggcccggtaa aaggggcgcg cgtacatctg
     1501 gcaatgccga aaccgtcttt attcgtccgt gaacaaaaat ccccttctgc atcggtgacg
     1561 gtaaatctgt tacccggccg cgcactcgat gaagggcaaa ttagcgccat tgtgcatctg
     1621 gtttccagcg ccgttgctgg tctgccgccg ggaaacgtca cgctggtgga tcagggcgga
     1681 catctgttaa cccagtccaa taccagctgg cgcgatctta atgacgctca attgaaatat
     1741 gccagcgatg tcgaaggccg tattcagcgg cgtattgaag caatcctgtc gcctattgtt
     1801 ggtaacggta atattcacgc ccaggtcacg gcgcaactgg acttcgccag taaagaacaa
     1861 acggaagaac agtatcgccc taatggtgat gaatctcagg cggcgcttcg ttcacgccag
     1921 cttaatgaga gcgagcaaag cggttccggt tatccgggcg gcgtaccggg ggcgttgtcg
     1981 aatcaaccgg ctcctgcgaa taacgcgcca atcagcacgc ctccggcaaa tcaaaataac
     2041 cgccagcagc aggcgagcac caccagcaat agtgggccgc gtagcacaca gcggaatgaa
     2101 accagtaact acgaagtcga tcgcactatt cgtcatacca aaatgaacgt gggcgatgtg
     2161 caacgtctgt cagtcgcggt agtggtgaat tacaaaacct tgccagacgg taaaccattg
     2221 cctctcagca acgaacagat gaaacaaatt gaagatctga cccgcgaggc gatgggcttt
     2281 tctgaaaaac gcggtgactc gctcaatgtc gttaactcgc cgttcaatag tagtgacgaa
     2341 agcggcggag aactgccgtt ctggcaacag caagcgttta tcgatcagtt actggctgcc
     2401 ggtcgctggt tgctggtact gctggtggcg tggctgctgt ggcgtaaagc ggtacgcccg
     2461 cagctaacac gtcgcgctga ggcgatgaaa gctgtacagc aacaggcgca ggcccgcgag
     2521 gaagtggaag atgcggtgga agtccgcctg agcaaagacg aacaactcca acaacggcgc
     2581 gctaaccaac gtctgggggc agaagtcatg agccagcgta tccgtgaaat gtctgataac
     2641 gatccgcgcg tggtagcgct ggtcattcgc cagtggataa ataacgatca tgagtaacct
     2701 gacaggcacc gataaaagcg tcatcctgct gatgaccatt ggcgaagacc gggcggcaga
     2761 agtgttcaag cacctctccc agcgcgaggt gcaaaccctg agcgctgcaa tggcgaacgt
     2821 cacgcagatc tccaacaagc agctaaccga tgtgctggcg gagtttgagc aagaagctga
     2881 acagtttgcc gcactgaata tcaacgccaa cgattatctg cgttcggtat tggtcaaagc
     2941 tctgggtgag gaacgtgccg ccagcctgct ggaagatatt ctcgaaactc gcgataccgc
     3001 cagcggtatt gaaacgctca actttatgga gccgcagagc gccgccgatc tgattcgtga
     3061 tgagcatccg caaattatcg ccaccattct ggtgcatctg aagcgcgccc aggccgccga
     3121 tattctggcg ttgttcgatg aacgtctgcg ccacgacgtg atgttgcgta tcgccacctt
     3181 tggcggcgtg cagccagccg cgctggcgga actgaccgaa gtactgaatg gcttgctcga
     3241 cggtcagaat ctcaagcgca gcaaaatggg cggcgtgaga acggcagccg aaattatcaa
     3301 cctgatgaaa actcagcagg aagaagccgt tattaccgcc gtgcgtgaat tcgacggcga
     3361 gctggcgcag aaaatcatcg acgagatgtt cctgttcgaa aatctggtgg atgtcgacga
     3421 tcgcagcatt cagcgtctgt tgcaggaagt ggattccgaa tcgctgttga tcgcgctgaa
     3481 aggggccgag cagccactgc gcgagaaatt cctgcgcaat atgtcgcagc gtgccgccga
     3541 tattctgcgc gacgatctcg ccaaccgtgg tccggtgcgt ctgtcgcagg tggaaaacga
     3601 acagaaagcg attctgctga ttgtgcgccg ccttgccgaa actggcgaga tggtaattgg
     3661 cagcggcgag gatacctatg tctgataatc tgccctggaa aacctggacg ccggacgatc
     3721 tcgcgccacc acaggcagag tttgtgccca tggtcgagtc ggaagaaacc atcattgaag
     3781 aggctgaacc cagccttgag cagcaactgg cgcaactgca aatgcaggcc catgagcaag
     3841 gttatcaggc gggtattgcc gaaggtcgcc agcaaggtca taagcagggc tatcaggaag
     3901 gactggccca ggggctggag caaggtctgg cagaggcgaa gtctcaacaa gcgccaattc
     3961 atgcccggat gcagcaactg gtcagcgaat ttcaaactac ccttgatgca cttgatagtg
     4021 tgatagcgtc gcgcctgatg cagatggcgc tggaggcggc acgtcaggtc atcggtcaga
     4081 cgccaacggt ggataactcg gcactgatca aacagatcca acagttgttg cagcaagaac
     4141 cgttattcag cggtaaacca cagctgcgcg tgcacccgga tgatctgcaa cgtgtggatg
     4201 atatgctcgg cgctacctta agtttgcatg gctggcgctt gcggggcgat cccaccctcc
     4261 atcctggcgg ctgtaaagtc tccgccgatg aaggcgatct cgacgccagt gtcgccactc
     4321 gctggcaaga actctgccgt ctggcagcac caggagtggt gtaatgacca cgcgcctgac
     4381 tcgctggcaa gaactctgcc gtctggcagc accaggagtg gtgtaatgac cacgcgcctg
     4441 actcgctggc taaccacgct ggataacttt gaagccaaaa tggcgcagtt gcctgcggta
     4501 cgtcgctacg ggcgattaac ccgcgctacc gggctggtgc tggaagccac cggattacaa
     4561 ttgccgctcg gcgcaacctg tgtcattgaa cgccagaacg gcagcgaaac gcacgaagta
     4621 gaaagcgaag tcgttggctt taacggtcaa cggctgtttt taatgccgct ggaggaagtg
     4681 gaaggtgtcc tgcccggcgc gcgggtttat gccaaaaaca tttcggctga aggactgcaa
     4741 agcggcaagc agttgccgct cggtccggcg ttattaggtc gcgttctgga cggcagcggt
     4801 aaaccgctcg atggcctgcc ctcccccgat acgacggaaa ccggtgcgct gattaccccg
     4861 ccatttaacc cgttgcaacg tacaccgatt gaacatgtgc tggacaccgg cgtgcgccca
     4921 atcaatgccc tgcttaccgt tgggcgtggg cagcgtatgg ggctgtttgc cgggtccggc
     4981 gttggtaaaa gtgtgctgct ggggatgatg gcacgttaca cccgcgccga tgtcattgtc
     5041 gtgggtttga ttggtgaacg tgggcgcgaa gtaaaagatt ttattgagaa tatcctcggt
     5101 gccgaagggc gtgcacgatc agtggtgatt gccgctccgg cggatgtgtc tccgctcctg
     5161 cgaatgcaag gtgccgccta tgccacgcgc attgccgaag attttcgcga tcgtggtcag
     5221 catgtgttgc tgattatgga ctccctcacc cgctacgcga tggcccagcg tgaaattgcg
     5281 ctggcgattg gcgaaccacc cgccaccaaa ggttatccac cgtcggtgtt tgccaaatta
     5341 ccggcactgg tcgagcgtgc cggaaatggc attagcggcg gcggctcgat taccgcgttt
     5401 tataccgtgc ttactgaagg cgatgaccag caggacccca ttgcagactc cgcgcgggcg
     5461 atcctcgacg gtcacattgt gctgtctcgc cgactggcgg aagccgggca ctatccggct
     5521 atcgatattg aagcgtcgat cagccgcgca atgacggcgt tgatcagtga gcaacattac
     5581 gcgcgagtgc gcaccttcaa acagctgttg tcgagttttc agcgtaaccg cgatctggtt
     5641 agcgtcggcg cgtatgccaa aggcagcgat ccgatgctcg acaaagccat cgccctgtgg
     5701 ccgcagctgg agggctattt gcaacaaggc atttttgaac gcgcggactg ggaagcgtct
     5761 ctccaggggc tggagcgtat tttcccgaca gtgtcataac ccaggagata acggcagatg
     5821 gcagaacatg gcgcgctggc gaccctgaaa gatctggcag aaaaagaggt agaggatgcc
     5881 gcgcgcctgc tgggtgaaat gcgtcgcggg tgtcagcagg cggaagaaca gctcaaaatg
     5941 ctgatcgatt atcagaatga atatcgcaat aacctcaaca gcgatatgag tgccgggatg
     6001 accagcaacc gctggatcaa ctatcagcag tttatccaga cgctggaaaa agccattact
     6061 cagcatcgcc agcaacttaa tcagtggacg cagaaagttg acattgccct gaacagctgg
     6121 cgagaaaaga aacaacgttt gcaggcctgg cagacactgc aggaacggca atccacggcg
     6181 gcactgcttg cagaaaaccg cctcgatcag aaaaagatgg atgagttcgc ccagcgcgcc
     6241 gccatgagga aacctgaatg attcgcttag cgcccttgat taccgccgac gttgacacca
     6301 ccacattgcc tggcggcaaa gccagcgatg ctgcacaaga ttttctcgcg ttgttgagcg
     6361 aagcattagc aggcgagaca actaccgaca aagcggcccc ccagttgctg gtggcaacag
     6421 ataagcccac gacaaaaggc gagccgctga tcagcgatat tgtttccgac gcgcaacaag
     6481 ctaatttact gatccctgtg gatgaaacac cgcctgtcat caacgacgaa caatccacat
     6541 caacaccgtt aaccaccgct cagacgatgg cgttggctgc ggtggctgac aaaaatacga
     6601 caaaagacga aaaagcggat gatctgaatg aagacgtcac cgcaagcctg agcgcccttt
     6661 ttgcgatgtt gccgggtttt gacaatacgc ccaaagtgac tgatgtgccg tcaaccgtgt
     6721 taccggcaga gaaaccaacg ctcttcacaa aactgacttc tgcgcaactc acaacagcac
     6781 agcctgatga tgcccccggc acaccagctc agccattaac accgcaggta gcagaagccc
     6841 agagtaaagc ggaagtcatc agcacgcctt cgccggtgac tgctgccgcc agcccgctaa
     6901 tcactccaca ccagacacag ccactgccca ccgtcgccgc gcctgttttg agtgcaccgc
     6961 tgggttctca cgaatggcaa caatcattaa gccagcatat ttcgctgttc acccgccagg
     7021 ggcaacaaag tgcagagttg cgtctgcacc cgcaggattt aggtgaagtg caaatctccc
     7081 tcaaagtgga tgataaccag gcgcaaatcc agatggtttc atcgcatcag catgtacgcg
     7141 ccgccctgga agcagcgctg ccggtactgc gtacgcagct ggccgaaagt ggcattcagt
     7201 tagggcaaag caacatcagt ggcgaaagct ttagtggtca gcagcaggcc gcttcccaac
     7261 aacagcaaag ccaacgcaca gtaaaccatg aacctctggc gggggaagac gacgatacgc
     7321 ttccggttcc cgtctcttta caagggcgcg taacaggcaa cagcggcgtt gatattttcg
     7381 cctaacgtca gaggtagcac cgtaatccgc gtcttttccc cgctttgttg cgctcaagac
     7441 gcaggataat tagccgataa gcagtagcga cacaggaaga ccgcaacaca tgactgatta
     7501 cgcgataagc aagaaaagca agcgatcgct ttggatcccg attctggtat tcattaccct
     7561 cgcggcctgt gccagcgcag gttacagcta ctggcattcg catcaggttg ctgctgacga
     7621 caaagcgcag caacgcgtcg tgccctcccc ggtcttctat gcgctggata cctttacggt
     7681 caatctgggc gatgcggatc gcgtgcttta tatcggcata accctgcgcc tgaaagatga
     7741 agctacccgc tcgcggctga gtgagtattt gccggaagtc cgtagccgtt tgctgttact
     7801 gttttcgcgt caggatgctg ccgtactggc gacagaagaa ggcaagaaaa acctgattgc
     7861 cgagattaaa accacacttt ccaccccgct tgttgccggg caaccgaaac aggatgtcac
     7921 cgacgtgctg tataccgctt ttattctgcg ataacgacat gggcgatagt attctttctc
     7981 aagctgaaat tgatgcgctg ttgaatggtg acagcgaagt caaagacgaa ccgacagcca
     8041 gtgttagcgg cgaaagtgac attcgtccgt acgatccgaa tacccaacga cgggttgtgc
     8101 gcgaacgttt gcaggcgctg gaaatcatta atgagcgctt tgcccgccat tttcgtatgg
     8161 ggctgttcaa cctgctgcgt cgtagcccgg atataaccgt cggggccatc cgcattcagc
     8221 cgtaccatga atttgcccgc aacctgccgg tgccgaccaa cctgaacctt atccatctaa
     8281 aaccgctgcg cggcactggg ctggtggtgt tctcaccgag tctggtgttt atcgccgtgg
     8341 ataacctgtt tggcggcgat ggacgcttcc cgaccaaagt ggaaggtcgc gagtttaccc
     8401 ataccgaaca gcgcgtcatc aaccgcatgt tgaaactggc gcttgaaggc tatagcgacg
     8461 catggaaggc gattaatccg ctggaagttg agtacgtgcg ttcggaaatg caggtgaaat
     8521 ttaccaatat caccacctcg ccgaacgaca ttgtggttaa cacgccgttc catgtggaga
     8581 ttggcaacct gaccggcgaa tttaatatct gcctgccatt cagcatgatc gagccgctac
     8641 gggaattgtt ggttaacccg ccgctggaaa actcgcgtaa tgaagatcag aactggcgcg
     8701 ataacctggt gcgccaggtg cagcattcac agctggagct ggtcgccaac tttgccgata
     8761 tctcgctacg cctgtcgcag attttaaaac tgaaacccgg tgacgtcctg ccgatagaaa
     8821 aacccgaccg catcatcgcc catgttgacg gcgtcccggt gctgaccagt cagtacggca
     8881 ctctcaacgg tcagtatgcg ttacggatag aacatttgat taacccgatt ttaaattctc
     8941 tgaacgagga acagcccaaa tgagtgacat gaataatccg gccgatgaca acaacggcgc
     9001 aatggacgat ctgtgggctg aagcgttgag cgaacaaaaa tcaaccagca gcaaaagcgc
     9061 tgccgacgcg gtgttccagc aatttggcgg tggtgatgtc agcggaacgt tgcaggatat
     9121 cgacctgatt atggatattc cggtcaagct gaccgtcgag ctgggccgca cacgaatgac
     9181 catcaaagag ctgttgcgtc tgacgcaagg gtccgtcgtg gcgctggacg gtctggcggg
     9241 cgaaccactg gatattctga tcaacggtta tttaatcgcc cagggcgaag tggtggtcgt
     9301 tgccgataaa tatggcgtgc ggatcaccga tatcattact ccgtctgagc gaatgcgccg
     9361 cctgagccgt tagtgatgaa taaccacgct actgtgcaat cttccgcgcc ggtttctgct
     9421 gcgccactgc tgcaggtgag cggcgcactc atcgccatta ttgccctgat cctcgctgct
     9481 gcctggctgg taaaacggtt gggatttgcc ccgaaacgca ctggcgttaa cggtctgaaa
     9541 attagcgcca gtgcttcgct gggcgcgcgt gaacgggttg tggtggtcga tgtggaagat
     9601 gcacggctgg tgctcggcgt taccgcaggg caaatcaatc tgctgcataa acttccccct
     9661 tcagcaccaa cagaagagat accgcagacc gattttcagt cggtcatgaa aaatttgctt
     9721 aagcgtagcg ggagatcctg atgcgtcgtt tattgtctgt cgcacctgtc cttctctggc
     9781 tgattacgcc cctcgccttc gcgcaactgc cgggtatcac cagccagccg ctgcctggcg
     9841 gtggacaaag ctggtcgctc ccggtgcaga cgctggtgtt catcacctcg ttgacgttta
     9901 ttccggcaat tttactgatg atgaccagtt tcacccgcat catcattgtt tttggtttat
     9961 tgcgtaacgc gctgggaaca ccctccgcgc cacctaacca ggtattgctg gggctggcac
    10021 tgtttttgac cttttttatt atgtcaccgg tgatcgacaa aatttatgtc gatgcgtacc
    10081 agccattcag cgaagagaaa atatcaatgc aggaggcgct ggaaaaaggg gcgcagccgc
    10141 tgcgtgagtt tatgctgcgt cagacccgtg aggcagattt agggttgttt gccagactgg
    10201 cgaataccgg cccgttgcag ggacctgaag ccgtgccgat gcgcattttg ctcccggcct
    10261 acgtgaccag cgagttgaaa accgcatttc agataggctt cacgattttc atcccttttt
    10321 tgattatcga cctggtgata gccagcgtgt tgatggcatt ggggatgatg atggttcccc
    10381 ccgccaccat tgctctgccc tttaaactga tgctgtttgt actggtggat ggctggcaat
    10441 tgctggtcgg ttcgctggcg cagagctttt acagctagag aggcaaaatg acacctgaat
    10501 cggtcatgat gatggggact gaagcgatga aagtcgcgct ggcactggct gccccgctat
    10561 tgttggtagc gttggtcacg ggccttatca tcagtatttt gcaggccgcc acgcagatta
    10621 acgaaatgac gctgtcgttt attccgaaaa tcatcgccgt atttatcgcc attattattg
    10681 ccggaccgtg gatgctcaat ctgttgctgg attacgtccg caccttgttc actaacctgc
    10741 cgtatatcat cgggtagccg tactatgttg caggtgacaa gcgaacaatg gctatcctgg
    10801 ttaagcctgt acttctggcc gttactgcgc gtgctggcgc tgatctccac cgcgccgatt
    10861 ctgagcgaac gcagcgtacc gaaacgggta aaactgggtc tggcaatgat gatcacgttc
    10921 gccattgccc catcattacc tgccaacgat gttcctgttt tttcgttctt tgctctgtgg
    10981 ctggccgtgc agcagatcct gatcggcatt gcgcttggtt ttaccatgca atttgccttt
    11041 gccgctgtgc gaaccgctgg cgaaattatc ggtctgcaaa tggggctgtc atttgcgacg
    11101 tttgtcgatc cggccagcca tcttaatatg cccgttttag cgcgtatcat ggatatgctg
    11161 gcgttactgc tgttcctgac atttaacggt catttatggt tgatttcact gctggtcgat
    11221 acctttcaca ccctgccgat tggtggcgaa ccgttgaaca gcaatgcgtt tctggcactc
    11281 accaaagcag ggagtttgat tttccttaac gggctgatgc tggcgttacc gctcattact
    11341 ctgctgctga cactgaatct ggcattaggt ttacttaatc gtatggcccc gcaattatcc
    11401 atttttgtta ttggatttcc attaactctg actgtcggca tctctttaat ggcggcatta
    11461 atgccgttaa ttgcgccttt ttgcgaacat ttattcagtg aaatttttaa tttgctggct
    11521 gatattatta gtgaattgcc attaatataa ttccgtaacg tttatcatgt tatcctaagg
    11581 attatccgaa aaataatacc tacgaacatc ttccaggata ctcctgcagc gaaatatttg
    11641 ttttaagctc actcacatat cgcaacattt actttacttt aagacaattc caggcaaatt
    11701 atacaacact ttacgggata gtaagtccgc ctgaaaaatc gcgagagtgg cgcattaggt
    11761 gacccatgtt gttccgttta gtcatgatga aatattcagg taaggggaat tatcgttacg
    11821 cattgagtga gggtatgcca tgtcaacgat tattatggat ttatgtagtt acacccgact
    11881 aggtttaacc gggtatctgt tgagtagagg ggttaaaaaa agagaaatca acgacattga
    11941 aaccgttgat gaccttgcca tagcttgtga ttcacagcgc ccttcagtgg tgtttattaa
    12001 tgaggactgt ttcatccacg atgcttctaa cagtcagcgt atcaagctca tcattaatca
    12061 acatcccaat acgttattta tcgtttttat ggcaattgcc aatgttcatt ttgatgaata
    12121 tctattggtc agaaaaaatt tattgatcag ttctaaatcg attaaaccgg aatctctcga
    12181 cgatatcctt ggcgatattc tgaaaaaaga gacaacgata acctcgtttt taaatatgcc
    12241 gacgttatca ttgagccgaa ccgaatcgag tatgttgcga atgtggatgg caggtcaggg
    12301 aaccattcaa atctccgacc aaatgaatat caaagccaag accgtttcat cgcataaagg
    12361 taatattaaa cgtaagatca aaacgcataa taaacaggtt atctaccatg tcgtccgact
    12421 gacggataat gtgactaatg gtatttttgt caacatgcgc taacacattc tgactggtgg
    12481 tttcccacca gtcaggctga attagattac tctgctttct ccacaaagat accgtcctga
    12541 tgccctgctt cattaaagaa ccagatgccg agcgggtagt cttccagcga aaccaggtac
    12601 attgtgcctt cactaaactc ctcaactgcc agtaccacgc caggacgacg cggaccgcca
    12661 tccgttttga ctgttacccg atcattcacc ttcatcgttt tcctcctgtg gctttgtgcc
    12721 agtgtagaac aatttcgttt ttctggcagc gccaggcgcg cgcgagtgct gattttctcg
    12781 acggtctata cttaagagat gccaggcgga cttaacgact ggcggcaaca acagagtaac
    12841 ggttgcgagg aaagatgatg aaaaccgcaa aagagtacag cgataccgca aaacgtgagg
    12901 tcagcgtcga tgtcgatgcc ctgctggcgg cgatcaatga aattagcgaa agcgaagttc
    12961 atcgcagcca gaacgattct gaacacgtta gcgtcgatgg acgtgaatat catacatggc
    13021 gtgaattggc ggatgccttc gaactggata ttcatgactt cagcgtctct gaagtgaatc
    13081 gttgaatgca caataaaaaa atcccgaccc tgagggggtc gggatgaaac ttgcttaagc
    13141 aagaagcact taaaaaattc gttacaccag gaaatctgat gtgttcatca ccttatccgc
    13201 aatttttttc gctgacaaga aaatattcgc tatatgaatg attaacgcta cttatgaata
    13261 gaaatgtgac gcacgtcacc ccttggtggc tctggcgtca gcgggatcgc tcccgaaaaa
    13321 atcatctttg ataatttgtg ctgttttgag aatcatcctg gtaaggggag tactgcgcga
    13381 ctatgttttc aattcaacaa ccactactgg tttttagcga tcttgatggc accctgctgg
    13441 acagtcatag ttatgactgg caaccggcag ccccctggct cagccgttta cgcgaagcaa
    13501 atgttcccgt cattctctgt agcagtaaaa catcagcgga aatgctgtac ttgcaaaaaa
    13561 tgttggggct acaaggttta ccgctgattg cagagaatgg cgcagtgatc cagcttgctg
    13621 aacaatggca ggagatagac ggttttccac gcatcatctc aggtattagc catggcgaaa
    13681 tcagccaggt tttaaatacg ctacgcgaga aagaacattt taaattcacg acttttgatg
    13741 atgtcgacga tgcaaccatc gccgaatgga cgggattaag ccgtagccag gcggcgctga
    13801 cgcagcttca tgaggcgtcg gtaacgctaa tctggcgcga cagtgacgag cgtatggcac
    13861 aatttaccgc tcgtctgaac gaactgggct tacagtttat gcaaggtgcg cgcttctggc
    13921 acgtcctgga tgcctctgcc ggaaaagatc aggctgccaa ctggattatc gcgacctatc
    13981 aacaattgtc aggcaaacgc ccaaccacac ttggcctggg cgatgggcca aacgatgcgc
    14041 ccttactgga ggtaatggat tacgcggtga ttgtgaaagg gctaaaccgt gaaggggtgc
    14101 atctgcatga tgaggatccg gcccgcgtct ggcgaacgca gcgtgaagga ccggaaggat
    14161 ggcgtgaagg gctggaccat tttttctccg cccgttaagc gttatcgctc gcgaataccc
    14221 gattgcgccc agcctgttta gcgagataaa gccgacggtc ggccagtgac tggagttgtt
    14281 caaaatcata atcaccggtt tcctcgctgc tacttacccc caacgaggca ctgatgcgta
    14341 tcgtcgtact cttagcaatc aacatctctt tttcatttaa cttcaggcga atacgttctg
    14401 cgacttccgc agcctccgtc agactcgcgc ctggcagaat cacacaaaac tcctcaccac
    14461 cgacccgccc ggcaacgtcc tgcgcacgca aggaactgct aattaatccg gcagcatgag
    14521 aaagaacacg gtcgcccgcc tgatgaccaa agcggtcatt aatcgcttta aaatggtcaa
    14581 gatcgacctg aatgacagaa aaaggatgtt ggtgcgtctg acacaatttc gcgagcggac
    14641 gggctttttc gaacagtgcg ccacggttat ataaacgcgt taaggtgtcg tgccacgcct
    14701 gccactgcaa cgagctttgc agaacataca tgttgctgac catccggcga atcacatacc
    14761 aggagatgag taacatggtg gtaaagagcg cccacagcag ggttaatgca atgctgatac
    14821 tgccgaaatc gccgcgcacg ccttcgctta acgtatggac gcgcaccagc acaccgtcga
    14881 aatggtccag acgttcccag ctaacatagc gactgtccat acgaatgccg ccccgcgtgt
    14941 catgttccat tgcctgcgcc agcaaggcca gttcacgagg atcaaaaata ttccctgttg
    15001 gatggtcagg attggaagag gtcaaaaatc tcagcttact gtcatagagc tgatactcac
    15061 catcgaggtt tttatcgatg gcgtttctta aaaattgctg catagtacgc acgggaatac
    15121 tcatccccag cacgccatac cagtagttat tactgtctac cggaacactg acggtaacct
    15181 gcggttcagt attgctggcg tgttccggtt gcgaagtgaa ccagcgtacc gcgcggtgac
    15241 gattttctcg ttgtgaatgg ccgataaacc agggttgggt gacatagccg taataacgcg
    15301 tcgggacatt gcgcgtaaac aaggtcggct gcgtcgaaac gtaaaatccg gcacgcgaga
    15361 catacatcgc ctgttctacc atcgacgagg agttgtgtgc cagtcgcagc aagtaaccaa
    15421 cttccagtgc agcggtaatt tcattgtcga ggctttcatt ttcgcgagac aggagattcc
    15481 cctcgctgac taatgcatcc gatacgccat tgactgacag ggtgcgtcgt cggttgagtt
    15541 cgatttgcca ggcgtgctcg tcgcgatgct gttcgaactc ggttacagca tcacgcagag
    15601 aggtgaaatc cagtggcgct acgagagctt cgcgcatgcc attacgcaaa aagatcagtt
    15661 tgtcgacgtt atactgcaaa tgcttatcga gcgcgttggc tacgttttcc agatgattac
    15721 gctggctgga gatataggca tcttccagca ccactacttc acgccaggtc agcaaggtgg
    15781 aaaaaagcaa taccacgata aagcagagat taacgatatg accaggccct aggcggcgtg
    15841 cgagtttttt caaccagctc tggttttcca tttttgtctc gtgctgcacc ctgacgactc
    15901 cccacctgct tttttatgat tctggcataa cctggcgata gcgtaccgca aaacagcgat
    15961 aattttataa tggtggcatg ataagacgcg actatcattc attgcaggca ttggcacacc
    16021 aggccggatg tgatccggcc tggtcttaat gatacccttt caaacttctt ccgcagaacg
    16081 actcccctcc cctggaacaa gctcggtttc atgaaaagct tcccgcttga ccccgtaacc
    16141 gtcataccaa cgacactcaa ccataccgct ggagtatcca gtgacaatca tccgcgggcc
    16201 gccctcttta accgtaactt cctcactaac cataaagctc atactcgcct ccttttttcg
    16261 agtgaaactt gttcacctta gttgaagatg gcgaattttg cataatagcc tgtgcgagta
    16321 ttaatgtacc tgaccgcgca ttttcgctac ggctttcact cccagcacca cgatgccgcc
    16381 gatgataaat ccaagaatca gatttaaaac agtcggtaat atcatcgcca ccactgcact
    16441 ttgctgcccg gcgaaatgtt caatggcgtg atgcagcggc gcaataccat gtaccacaat
    16501 cccgccgccg acgaggaaca tcgccagcgt gccgacaatc gataacgctt tcatcagcca
    16561 gggcgcgata atcaataatc ctttacctaa tgcctgcatc agcgcgctgg atttttccgc
    16621 cagccaatac cccaggtcat caatcttaac gataacccct accagaccgt agacgcccac
    16681 ggtcactacc agcgcgatgc ctgaaagcac cagcacctga ttaagcaacg gcgcttcggc
    16741 cacaatcccc agcgtgatgg cgacgatttc cgcagacaat ataaaatcgg tacgaatcgc
    16801 cccttttatt ttgtcctttt caaacttcag cggatcctgc gccgccagct tctccagacg
    16861 ctgctggctc tgcgccggat cttctttatg tttacgcgcc tccagcatat gcagcacttt
    16921 ctctacccct tcaaagcaga gaaacgcgcc accgatcatc aacaatggcg taatcgccca
    16981 cgggataaac gcactgatga tcagcgccag tggcaccaga atcactttat taatcagcga
    17041 tcctttcgcc acgccccaga ccacgggaag ttcccggttg gcccgcacgc ctgaaacttg
    17101 ttgcgcattg agcgataagt catcccctaa tacaccggcg gttttcttcg ccgccagttt
    17161 gcccatcacg gagatatcgt ccagcagtgt ggcgatatca tcgagcaacg tcagtaaact
    17221 actcccggcc aaaagcattc cctctcattt ttattgttga agggtgtaag tatgaagcaa
    17281 aaacggcagc tctgatactt gcttcacagg tcaacatttt tttaacaatc aagtgcaatt
    17341 atatctctcg ccagcagaat gattttcacg tttactatga accacctttt tatttccacc
    17401 gtgagggatt atgcgtttcc gccagttgtt accgcttttt ggcgcgctgt ttgcgttgta
    17461 tatcatttgg ggctcaacct attttgtcat tcggattggc gtggaaagct ggcctccgtt
    17521 aatgatggcg ggtgttcgat tcctggcagc cggtatttta ttgctggcat ttttactact
    17581 gcgcggacac aaactccccc cgctacgtcc gctgctcaat gccgcgctga ttggcctgtt
    17641 attgctggct gtcggtaatg gcatggtgac ggttgccgaa catcaaaatg ttccttccgg
    17701 catcgccgcc gtagtggttg caaccgtgcc cctctttacc ctgtgcttca gccgcctgtt
    17761 tggcattaaa acgcgcaaac tggaatgggt gggtattgcc attgggcttg ccggaatcat
    17821 catgctcaat agcggtggaa atttaagcgg caatccgtgg ggcgcgattc tgattttaat
    17881 cggctcgatt agctgggcgt ttggctcagt ttatggctcg cgcattacct tacctgtagg
    17941 gatgatggcg ggtgcgattg agatgctggc ggcaggcgtg gtgttaatga tcgcgtcgat
    18001 gattgcgggt gaaaaactga cggcgctccc ttccctttca ggcttccttg cggtcggcta
    18061 tctggcgctg tttggttcga ttatcgccat caacgcttat atgtatttaa tccgtaatgt
    18121 cagtccggct ctcgccacca gctacgctta cgttaacccg gtggtcgcgg tcttgctggg
    18181 tacgggactg ggtggagaaa cactgtcgaa gattgaatgg ctggcgctcg gcgtaattgt
    18241 cttcgcggtg gtactggtca cgttgggaaa atatctcttt ccggcaaaac ccgtagttgc
    18301 tccagttatt caggacgcat caagcgagta aatgtatccc ctgcgtgtcg atctgcgcgc
    18361 tggctccttc gccgcagatc cactcttcca gacgctcggt aagcgcttca tccttcagct
    18421 tctcgcgccc acgtaacgcg cactcccaga caatcaatac gcgccagccg agttcctgca
    18481 agcgactgat atcgcggcga tcgcgctcaa catttttacc tatcttctcc agccagaact
    18541 cggttcgagt cgcaggcact ttaaacagat agcagtgatg atgatgccag aagcagccat
    18601 gggtaaatat cacgcagcga tattcatcaa cgacaaaatc cgggcgtccg ggcagactgg
    18661 cgtcctgaac gcgaaacgcc aggccctgcc cggttaacag actggcgagg cgcttctcaa
    18721 tcgccgtatc acgcgtggca atcgcgcgca tatttttgct gcgagtggcc ttatcgtgaa
    18781 cgtcggccat gttgtgcctc ttgctgacgc aacgccaccg cctgtttgat ttttggctca
    18841 agcagttttg ccaccgcggc aaagaccggc acgaccaccg agttaccgaa ctggcgatag
    18901 gcctgagtgt ccgaaaccgg aatgcggaat ttcgcttctc ccggcgcttc aaaacccatt
    18961 aagcgcgcgc attcccgagg cgttaaccga cgtggacgat gttgctgatt cagcggatcg
    19021 tcaaagtctt tctcacccgt ggccatatcc cagccgcgat cgattaaaat ttccgcgcca
    19081 tctttgtaat aacgcgcaga cagcgtacgg gtgacgcttt gcggattgtt cggataaacc
    19141 attccataac cgaagccgtt tccgcgcgcc tgatgttttt tcgcatatcg atagaggtac
    19201 ttccacagca ccggcgtcag gatatatttc gcctcgacca tcgggtccaa cagctgcgcc
    19261 agcgtcactc gctgtgcagg gaaacattcg ctgatatcac gcagagtaaa atcggctttc
    19321 agattaagat cgcgacgaaa acccaccagc acgatgcgtt cacggtgctg cggcagaaaa
    19381 tgtttgccat cgatgatttt cggatcgtcc ggcccgttat cttctgcatc agccacgtca
    19441 tagcccagtt cgtccagcgt ctgcatgatg atgcggaacg ttttaccctg gtcgtggctt
    19501 ttcaggtttt tgacgttttc gagcacaaac atcgccggac gacgcgcgtc gataatgcgc
    19561 accacatcaa agaacagcgt gccctgagta tcgcaggcaa aaccgtgcgc ccgcccgagc
    19621 gagttctttt tcgatacgcc agccagcgaa aatggctgac aagggaaacc ggccagtaaa
    19681 acatcgtgtt caggaatgtg ttgacgaata tgttccgccg cagcctcatc actcacgcct
    19741 tctttatggc tgagggtgat gtcgcggata tcttcattaa aatgatgcgt cgccgggtcg
    19801 caataatgat tggctttata agtgcgtacc gcatgtttgt tccattcgct ggtaaacacg
    19861 cactgtccgc caatcgattc aaaaccgcga cggatgccgc caattccggc aaatagatcg
    19921 ataaagcgaa acgcataatg cggatgatgt gccggtggtt tgggtaacaa cgtttgcaga
    19981 tgggcgaact ctttctcact taaacggtgc catgccgagt cattcgccag cgcacgtttt
    20041 aaaattgccg cgctccagtg attctcaccc acaccattaa gctgagccac cagcgtttta
    20101 acatcataaa tttgcagcag tttctccagc attgcctgtg cggcattccc ggtgctgtat
    20161 gaatcggtta ctgatatatt ttcctgcata gatttaaccg gccatctgag atggtcgaca
    20221 ggttaacata attacagcca tttagggcca cgcgctcagc ccgcgggtga aaacgcatct
    20281 atcaccttgt gatcgacacc ttcattctcc cccgccagtt cggcactgag cttcgccata
    20341 aactcgacta aaaaatgcgc gttgtgctgc gccagctgct tgcctcttac ggtttgcatg
    20401 atttgcggta gcttcaacaa cttagtctgg aaatgatcca gcgcgtagcg tttatcatca
    20461 agcgggcgat gctgtgcgag cggatcttcg gcatcaaaca gcgccacgcc caatgcccct
    20521 gaaacggcaa acacgcgcgc caggccaatc gcccccaggg cttccagccg atccgcatcc
    20581 tggacgattt tagcttccgt tgttaagggg gcgatttgcg cgctgaaact gtgagcggca
    20641 atggcatgac aaacggcctc gattttctcc gccggaaatt gcacgaactc ttcacggagc
    20701 aggcgacgcg tctcttctgc cgccaggatt gaagaactct gccgttgcgg atgatttttc
    20761 gccaggctca caatatcgtg gaaataacag gcggttaaaa tcaccagcat atcgacgtca
    20821 tcatctgccg ccagcttctg tgccgtagcc cagacgcggc gaaaatgaca cacgtcatgg
    20881 gccgcgtcct ggtgctgatg atggtttttc aaccagtttt cgaactgtgc ctgccagtgt
    20941 tgtaagtcca tacgcgctcc gttgccaaag agtcgccacc ttagcagttt ttattcatag
    21001 cgtctgctac gtgcgggacg aggtcgtgtg taccaggcaa tgctgcctaa caatgccccg
    21061 accagagcca taaataaaaa tccgaccagt gccctaacca ctttcccgcg gtagagccta
    21121 aatcgctttc aaatcccgac acgggggcat cggggatccg gctcatcacc cagatgtaac
    21181 gcgccattgc ccagacgaaa tagccacacc atgtataaaa agcccacaaa gccagtttgc
    21241 cgccagggct gcgagagagt ctctcttcca tctttgtcga accgtattcc attttgagat
    21301 aacccggaaa attcagactt agtactatta cgtgataaaa gtcacatttt tccacattgg
    21361 atgagccagt caagactcac tcattttttg aacaaataga taatttttgc gcaacggcag
    21421 tgaatacagg aaaaatcgtt ttgagcgatt ttatttatct ttgagatcgg ctttattaat
    21481 tc
//
LOCUS       DAZFAB010000079        21229 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZFAB010000079 DAZFAB010000000
VERSION     DAZFAB010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21229
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.079"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..684)
                     /locus_tag="ACHJWA_003321"
     CDS             complement(<1..684)
                     /locus_tag="ACHJWA_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFO4309856.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNFRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGK"
     gene            complement(695..1696)
                     /gene="ltaE"
                     /locus_tag="ACHJWA_003322"
     CDS             complement(695..1696)
                     /gene="ltaE"
                     /locus_tag="ACHJWA_003322"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFO4309857.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKDAWEFTRERN
                     LALHVDGARIFNAVVAYGSELKELTQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMTGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(1733..3451)
                     /gene="poxB"
                     /locus_tag="ACHJWA_003323"
     CDS             complement(1733..3451)
                     /gene="poxB"
                     /locus_tag="ACHJWA_003323"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFO4309858.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEID
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(3584..4552)
                     /gene="hcr"
                     /locus_tag="ACHJWA_003324"
     CDS             complement(3584..4552)
                     /gene="hcr"
                     /locus_tag="ACHJWA_003324"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFO4309859.1"
                     /translation="MTMPTNQCPWRMQVHHIKQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLTRVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAQEFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(4564..6216)
                     /gene="hcp"
                     /locus_tag="ACHJWA_003325"
                     /gene_synonym="priS"
     CDS             complement(4564..6216)
                     /gene="hcp"
                     /locus_tag="ACHJWA_003325"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFO4309860.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(6360..7259)
                     /gene="lysO"
                     /locus_tag="ACHJWA_003326"
     CDS             complement(6360..7259)
                     /gene="lysO"
                     /locus_tag="ACHJWA_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFO4309861.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVASSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQHTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(7717..8412)
                     /gene="aqpZ"
                     /locus_tag="ACHJWA_003327"
     CDS             complement(7717..8412)
                     /gene="aqpZ"
                     /locus_tag="ACHJWA_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFO4309862.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLTAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRN"
     gene            8838..10496
                     /gene="ybjD"
                     /locus_tag="ACHJWA_003328"
     CDS             8838..10496
                     /gene="ybjD"
                     /locus_tag="ACHJWA_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFO4309863.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQAHHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(10493..11449)
                     /gene="ybjX"
                     /locus_tag="ACHJWA_003329"
     CDS             complement(10493..11449)
                     /gene="ybjX"
                     /locus_tag="ACHJWA_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFO4309864.1"
                     /translation="MNMSQLTERTFTSSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            11600..12715
                     /gene="macA"
                     /locus_tag="ACHJWA_003330"
     CDS             11600..12715
                     /gene="macA"
                     /locus_tag="ACHJWA_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFO4309865.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            12712..14658
                     /gene="macB"
                     /locus_tag="ACHJWA_003331"
     CDS             12712..14658
                     /gene="macB"
                     /locus_tag="ACHJWA_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFO4309866.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTASGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFMTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(14731..14955)
                     /gene="cspD"
                     /locus_tag="ACHJWA_003332"
     CDS             complement(14731..14955)
                     /gene="cspD"
                     /locus_tag="ACHJWA_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFO4309867.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            15105..15179
                     /gene="yljB"
                     /locus_tag="ACHJWA_003333"
     CDS             15105..15179
                     /gene="yljB"
                     /locus_tag="ACHJWA_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFO4309868.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            15360..16598
                     /locus_tag="ACHJWA_003334"
     CDS             15360..16598
                     /locus_tag="ACHJWA_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4309869.1"
                     /translation="MSRAIHRLSDTLLRKLSGSPTTKSTFFNDGGNLSVRHSTNGLLT
                     WYFTYRSGTGRQISPERLRLGNYPDLSLKAAREKASQCRAWLAEGKNPRYELNHAVQD
                     ALAPVTVKEALTYWLESYAKEKRTDYESLKSRINKHIISQIGAMPLEKCELRHWLACF
                     DQLAKRNPVSAGFLLQVCKQALKYCRKRRYAISNVLDDMVVGDVGKKAEISERVLTNK
                     ELGELLRALDEKIYPPYYSALIRLLIVFGCRTTELRRSEVQEWDFKEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVMKLVEQNRHTGLLLGELKGQSSVSEYGRTAHRRINQAPWTL
                     HDIRHTFTTMLNDLGVEPHVVEQLTAHQLPGMQRVYNHSRYLDAKRDALNLWVERLEL
                     LQNNDKKIVVMTPRIYSQNS"
     gene            17009..17218
                     /locus_tag="ACHJWA_003335"
     CDS             17009..17218
                     /locus_tag="ACHJWA_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4309870.1"
                     /translation="MREINEDRVIREKECRELTGVCRTTRYEMEKQGRFPSRISLGGR
                     SVGWVKSEVMEWVKSRNRINSGKAA"
     gene            complement(17353..17532)
                     /locus_tag="ACHJWA_003336"
     CDS             complement(17353..17532)
                     /locus_tag="ACHJWA_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO4309871.1"
                     /translation="MATGPKNAKSQSVTARIAHEIIEGMENVKEEGESTGQFINAAMR
                     GEIKRRQRRKAKEQE"
     gene            17732..17932
                     /locus_tag="ACHJWA_003337"
     CDS             17732..17932
                     /locus_tag="ACHJWA_003337"
                     /inference="COORDINATES: protein motif:HMM:NF033153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO4309872.1"
                     /translation="MRNHITHPQGRDSHNLNKYIWRFIALSTAQPRVITIEATSEQEA
                     RQQSPDGCVMVFAARIRQGEIL"
     gene            17932..18159
                     /locus_tag="ACHJWA_003338"
     CDS             17932..18159
                     /locus_tag="ACHJWA_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001824715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4309873.1"
                     /translation="MHKIPFEVLIHSENALIKAREMNALLIKLIEVPEGGEESGVLMF
                     AAIQTLLSPVIDELDKAMAIHENNNVPHTGE"
     gene            18165..18464
                     /locus_tag="ACHJWA_003339"
     CDS             18165..18464
                     /locus_tag="ACHJWA_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO4309874.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGTVMIKGVAGRCVTY
                     RREGYEYDCVMPVYQFRRDFSLVQTAPHNVPTSREKARANIQEIKKMLNVFRGKK"
     gene            18461..20209
                     /locus_tag="ACHJWA_003340"
     CDS             18461..20209
                     /locus_tag="ACHJWA_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5906 domain-containing protein"
                     /protein_id="HFO4309875.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAEQWQEQVGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQMLAGRFDG
                     VCVHPESEIVHVWRGGVWCPVSTMELSREMVAIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPTGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTQPAPGENIRDNAPNFH
                     KWLEHAAGKDPRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            20498..20776
                     /locus_tag="ACHJWA_003341"
     CDS             20498..20776
                     /locus_tag="ACHJWA_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4309876.1"
                     /translation="MYTSGKFNKSLKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKNVEVES"
     gene            20773..21195
                     /locus_tag="ACHJWA_003342"
     CDS             20773..21195
                     /locus_tag="ACHJWA_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO4309877.1"
                     /translation="MTAQIAAYGRLVADPQLKTTSKGTQMTMASMAVPLTCSQADDGT
                     ATMWLSVLAFGRQADALAKHRKGELVSVAGNMQVSQWTGQNGETRQGWQVIADSVISA
                     RTARPGGKKGQQGQATDALNRAKQQAGNDDPYGDNIPF"
BASE COUNT         5441 a         5255 c         5625 g         4908 t
ORIGIN      
        1 cttaccgctc accgccataa aatgttcaaa ctgttgctga taactgagca cctctggtcc
       61 ggcggcttcg aagatgcggt gttcgttggc tggatggtct aacagcgcca ccagatagtg
      121 cagcaagttt tccagcgcga tgggcgtggt gcgtgaacgt acccagcgtg gcggcgttaa
      181 cactggcagg ttgtagacca tatcgcgcat gacttcgaac gctgctgaac ctgcaccaac
      241 gattattccg gcacgcaatt cggtcactgg tacattcgct tcacgaagaa tgtccgccgt
      301 agcctgacgc gcgcgcagat ggtccgactg ctcatgtggc ggggcctgca acgaactgag
      361 aaagattaat tgcttaactg gtacttcacg tagcgcatcg cggaagttga gagccacctg
      421 gcgctcctga gcgataaaat cgccgccttc gcccatgcta tgcaccagaa aatagaccgt
      481 atcgatatcc tgtaacaggg ccggaaggtt atccggccag ctgagatcga ctttatggca
      541 actgacgttt gccagttgca gctttgcaag cctgtcgaca tgacgtgccg ccgccaggat
      601 ctgatgccct tgctggctga gtgtgcgcac cagatgctga ccaatgtagc cactggcacc
      661 aagaactaaa atgcgttgcg gcacgtctcc ctccttaacg cgccaggaat gcgcgccagt
      721 gggcggcgac ttctgccagt tgttcgcgcg agacgtcaag atgggtcacc aggcggacaa
      781 tcggcgaggc gttaatcagt acgtttctcg ctttcatgta ttcgcctaac gcggcagcat
      841 tttcttcgcc gacgcgaaca aacagcatat tagtgtcctg acgcatcaca tccgcgcctg
      901 cttcacgcag ttgctccgcc atccaggcgg cgttgtcgtg atcttcctgc aaccgtgcga
      961 cgttattttt cagcgcatac atcccggcgg cagccagaat gccggactgg cgcatcccgc
     1021 cacctgtcat tttccgccag cgaatggcac gtttaatgta atcacgatta ccgacgagta
     1081 atgaaccgac tggcgtccca agacctttcg acaggcaaat ggtgaacgaa tcacaatatt
     1141 gcgtgagctc tttcagttcg ctgccgtaag ccaccacggc attaaagatg cgcgcaccgt
     1201 caacatgcag cgccagattg cgctcgcggg taaattccca tgcatcttta aggtattcac
     1261 gcggcaacac tttgccgttg tgggtgtttt ccagactgag taatttggtg cgggcgaaat
     1321 ggatatcgtc gggtttgatt ttcatcgcca ctttatccag cggtagcgtg ccgtcggcag
     1381 ccgcgtcgat gggttgcggt tgaatactgc ccagcaccgc cgcgccaccg gcttcaaaca
     1441 gatagttatg cgcggcctga ccgacaatat actcttcgcc gcgttcgcag tgactgagca
     1501 gagcgaccag gttggcctga gtgccggtag gcagaaaaat ggcggcttct ttaccggaaa
     1561 gctctgctgc atagtcctgc agagcattaa cggtagggtc gtctccgtaa acgtcgtccc
     1621 caaccggggc ggccatcatc gcttcgagca tggcgcggct cggtcgggtt acggtatcac
     1681 tgcgtaaatc aatcatggca tgtccttatt atgacgggaa atgccaccct ttttacctta
     1741 gccagttcgt tttcgccagt tcgatcactt catcaccgcg tccgctgatg attgcgcgca
     1801 gcatatacag gctgaaacct ttggcctgtt cgagtttgat ctgcggtgga atggctaact
     1861 cttctttggc gaccaccaca tccaccaata ccggaccgtc gatggagaag gcgcgttgca
     1921 gggcttcatc gatttcagag gctttttcta cacgaatacc cgtaattccg cacgcttcgg
     1981 caatgcgggc aaagttggtg tcgtgcagct cagtaccgtc tgtcaggtag cctccggctt
     2041 tcatctccat cgccacaaag cccagcacgc tgttattaaa gacgataatt ttcaccggca
     2101 gtttcatctg cactaccgag aggaaatcgc ccatcaacat gctaaaaccg ccatcgccgc
     2161 acatggcgac cacctgacgt tccggctcgg ttgcctgcgc gcccagcgcc tgcggcatgg
     2221 cgttagccat cgaaccgtgg ttaaacgaac ctaacaggcg acgcttgccg ttcattttca
     2281 gataacgcgc cgcccacacc gttggcgtgc cgacgtcaca ggtaaagatg gcgtcatcgg
     2341 cggcaaaatg actaatttgc tgcgccagat attgcgggtg aatggctttc tcgctcggtt
     2401 tagctaaatc gtccagccct ttgcgggcat cgcggtaatc ttccagcgct ttatccagaa
     2461 acttgcgatc ggctttttct tccaccaatg gcagcaatgc acgcagggtc gacttgatat
     2521 cgccgaccag cgccatatcc accttgctgt gtgcgccgat gctggctggg ttgatatcaa
     2581 tctgaataat tttggcatcg gtcgggtaga acgcacggta ggggaactga gtaccgagta
     2641 gcactaacgt gtcggcgttc atcatggtat ggaaacctga cgagaatccg attaacccgg
     2701 tcattccaac atcatacgga ttatcgtatt cgacatgctc tttaccgcgt agggcatgaa
     2761 caataggtgc tttaattttc ccggcaaact caactaactc tttatgcgcc cccgcgcagc
     2821 cgctgccaca catcagagcg atattgctgg aataacgcag cagttgcgcc agtttgcgta
     2881 actcttcttc ttccggcgtc acgactggct gtggcgcatg ataccagtgg gtagttgccc
     2941 cttctggcgc aggttttaac gccacgtcgc ccggtaacac gacaacggaa acgccacggt
     3001 taagcaccgc tttgcgcatg gcaatcgcca gcacttgtgg gatctgctcc gggctggaaa
     3061 ccagctcgca atagtgacta cattcgcgga atagctcttg tgggtgggtt tcctggaaat
     3121 agccgctgcc aatttcgctg gaaggaatat gagcggcaat cgccagtacc ggaacgtgat
     3181 tgcggtggca atcgaacagg ccgttgatta agtgcaggtt gccggggccg cacgatccag
     3241 cacagaccgc cagttctcct gaaagttgtg cttcagcgcc agcggcaaac gccgccactt
     3301 cttcatggcg ggtggacatc cactcgatgg tgcccatgcg attaagactg tcgctaagac
     3361 cgttcagaga gtcgcccgtg actccccaga tgcgtttcac gcctgccgat tcgagtgttt
     3421 tggcgatata agctgcaacc gtttgtttca tggttctcca tctcctgaat gtgataacgg
     3481 taacaagttt agttcatctg acggaggggg aagggatggg agagaaagga ggcactaacg
     3541 gttaaatagc ccgatgaaag gaatatcatc gggcataagg cgattatgcg agaaccaaat
     3601 ccccctgcgg atggcaggag caggccagta cgtaaccttc agcgatttcg gcgtcggtca
     3661 gcgtcattgt actgctcacc gtatattcac cggaaaccac tttcgtctta cagcagccgc
     3721 aaacacccgc gcggcaggcg gcgacaaccg gaacgttatt gctttccagc gcctccagca
     3781 gcgtggtgcc aacaggagcg taaaattctt gcgccggttg cagtttggtg aatttcagac
     3841 cgctggtcgc cgcttccgct actggggtga agaatttctc tttaaagaaa cgcgtcacgc
     3901 cgagtgcttt cacttcctgc tctacccaat ccatatacgg agccgggccg caggtcatca
     3961 cggtacgtga agctaagtca ggaacgcgag tcaggagttc gcgagtgaga cgaccagcga
     4021 taaagccttc ggtaacgtta ttttccgcca ccagcgttac cggatagtta cgccactcat
     4081 cggcgaaaat aacatcctgc ggcgtacgca cgttgtagat cacccgcaca tcggcctgtg
     4141 gacggttctt cgccagccag cgacgcatcg acataatcgg cgtgacgccg cagcctgcag
     4201 ccagcaacag gaatttatct tctgctttat cgtcgcaggt aaattccccc atcgcgtctg
     4261 aaagccagag ataatcaccg cgttttacat cgcgggtcag ccactgggag ccgacaccgt
     4321 catcaatccg ccgcacggtc agggtgatat attcactcac gcctggcgtg gaggaaatgg
     4381 tgtaagcacg cagcgtttcc gctgagttac gcacgctgac cagtgcatat tgcccggcgc
     4441 ggtatgggta gtaatcgtgg caaatcaggg aaatcgtcca cacatccggc gtttcttgct
     4501 taatgtgatg aacctgcatc cgccacgggc attgattcgt tggcatcgtc atcgacaaac
     4561 tccttacgcg ctcaacagtt gcttcatgtc ttcttcaaca gtggtgatag aacgcaggcc
     4621 gaatttctcg ttcagcaccg ccagcagatc tggtgtcagg aaaccaggtg cagtcgggcc
     4681 ggtgacgata tttttcacgc ccagagaaag cagcgtcagc agaatgacaa tcgctttctg
     4741 ttcaaaccag gagagcacca gcgacagcgg cagatcgttg acaccgcagc ccagtttttc
     4801 tgccagcgta accgccagaa taattgctga gtaagcatcg ttacactgac cggcatccac
     4861 cagacgaggc agaccttcga tatcgccaaa ctcaagtttg ttaaagcgat atttaccaca
     4921 ggcgagagtc aggatcaggc agtcatctgg cacgctggtg gcgaaatcgg tgaagtagtg
     4981 acgttcgccg cgtgcgccgt cacagccacc aagcaggaag atatgacgca gtttttcacg
     5041 gctcaccaga tcaattagcg tatccgctgc gccaagcagc gtctggcggc caaagcccac
     5101 ggtgatcagg tgcggaattt cgctgtatgg gaagcctgcc atttgttgcg cctgggcaat
     5161 gaccgcagag aaatcttcgc catccagatg acgcacgcca ggccagccaa cgatgctgcg
     5221 ggtccagata cgatcgtcat aagcgcctac ggttgggtcg atgatgcagt tcgaggtcat
     5281 cacgatgggg ccagggaaac gagcgaactc cacttgctga ttctgccagc cgctgccgta
     5341 gttaccgacc agatgcttga atttacgcag ctccgggtag ccatgcgcag gcagcatttc
     5401 gccgtgggtg tagacattaa cgcccgtgcc ttcggtctgt tccagcaggt tgtagagatc
     5461 tttgagatcg tgaccggaaa tcaggatgca tttacccgcc gtcgctttga cgttgacctg
     5521 ggttggcgtc gggtgaccgt atttaccggt ttcgcctgca tccagaatgc tcatcacttt
     5581 gaagttcatc tggccgattt ccattgaaca ctcaagaagc gcgttcatat cggcaggcca
     5641 ggtccccagc cacgccatga ttttatgata ctgggcataa atatcgttgt cgtattgacc
     5701 gagaacgtgt gcgtgttcca tataggccgc cgcacctttc aggccataca ggcacagcag
     5761 acgcaggccg agaatgtttt cgccaatcgc cgctttatct ttgttagggg taaattctgc
     5821 tgcctgacgt tgcagttcgc cgagatcgtc gctcaccagt tgcaggtcag ccatcgggtt
     5881 atcgacgcgg gcgttggcat ctacagccag gcattgtgct ttcagcgcct cgcgcaaggc
     5941 aatcgcttca cgagcgtagc cgacaatacg cggagaatcg aagttaacgt tggtcagggt
     6001 tgagaaaaag gcacgtggcg cgaagctgtc gacatcgtgg ttgatgatgc cgtattcacg
     6061 cgcttttacc gcccaggcag aaagcccttg cagcgccgcg atgagtaaat cctgaaggtc
     6121 agaagtttcc gccgttttac cacacatccc ctgcgcgtat gagcagccgt ttcctgccgg
     6181 agtacggata gtttgttcac attgcacaca aaacatgatc acacctttta aagttatatt
     6241 taatatacat gtttaaggtt aagacgctta acgcggggat aaaagggatt tttcatgcaa
     6301 ctttaaggga gattgattta gcgcaatttt ggcggcaggg atctaccgcc agagaggtat
     6361 tacgcggaga aaaaggcgat gaggatcggc actaacaggc taagaataaa accgtgaaca
     6421 attgccgccg ggaccatatc cagcccgcca gtatgttgaa gaacgggcag ggtgaaatcc
     6481 atcgacgtcg caccgcataa gcccagtgcg gtagagcggc tgcggcgaat cagcccaggg
     6541 atcaacataa ttgcaatcag ctcacgggcc agatcattaa aaaacgccgc gctcccgatt
     6601 accggaccaa aagattcggt caataaaata ccggaaagag aataccagcc gaaaccagag
     6661 gccattgcca gcgcggtatt gatgggtaga tcaagaataa aggcgttaat taaaccacca
     6721 attaatgaac tggcaaccac caccacggcg acaatcattc cccggcgatt aaggacaatc
     6781 tgctttaagg tcatgccatt attgcgcaac tgaataccga cgaggaaaag tagcagaatt
     6841 aacgtgtatt cactggcttc ggtcgcgtgt tgtaagaaag ccagtccact caggccaatg
     6901 gcaaaaccaa tcactactac gccgcacagt tttagcgact caagcgccat cgcaatacgc
     6961 gacgggagtt tttcttgctg atggtggttg cgccacggca gaccgcgctc cagccacata
     7021 agggcggcaa tattacacag taaaataacg gtaatactga cggcagaata atgcagaatc
     7081 gccaacaggt tactggcgag gttatcgaga aacgccagac tgatacccat aaaaaagaga
     7141 ataaggtaaa ccatccagct taatagctga ttaataactt ttaacgcagc ttgttggcga
     7201 agcggaatga ggtaacccac aatcagggga accagaatga ttaacaaccc agaaaacatg
     7261 aaaaaccagc ccttgcaaag aaagaagtcg aaatgcgcga tgacacacta ctgaaagcgg
     7321 aaggacgagt aaagttgcaa ttaaaaggaa atgttatgca tcagaggggg atttttaatt
     7381 ctatatgagt gtttagcatt gttgcgctcc tttcatgtgc aatgccatat aaagccactg
     7441 gcgcagggag cgcgcagggg gcggccagtc gccgccgccc cctgcacccc cgggctctgg
     7501 cgaacaaaat cgccgctgcg cggtaccttc agcttatccc cttcggcaac cgggtcgggc
     7561 gcgaggtaac gtcctgtaag acgcgccctc agcccacatc catgtgggct gccccggcct
     7621 tcggggaacg cttcagcgat tttgacgcca ccaatacccg tgcggtttgt aatgtcgaag
     7681 ataaaacaac cagggcagat aaaatgccaa tgctaattaa ttacgctttt ccagcagagt
     7741 ccggtaaatc agaccaccga taatgccacc gacaattggc accacccaga agaaccacag
     7801 ttgttctaat gcccagccgc cctggaagat ggcaaccgcg gtgctgcgcg ccgggttaac
     7861 agaagtgtta gtcaccggaa tactgattaa gtgaatcagg gttaaggcca gaccaatagc
     7921 gatcggcgca aaacctgccg gagcgaattt gtcggttgca ccgtggatca ctaacaggaa
     7981 gcccgcactc aataccagtt caactaccag cgcggaaagc atggaataac cgcctggtga
     8041 atgctcgcca taaccgttag aagcaaaacc gctggctgcc gcgtcaaaac ccgttttacc
     8101 actggcaatt aaatacagca gcgctgccgc aacaataccg ccgacaacct gggcaattac
     8161 gtagccaacg acttcttttg ccggaaaacg tccgccagcc cataaaccaa tagtgaccgc
     8221 cgggttaaaa tgaccaccag aaatatgacc aacagcaaag gccatcgtca gaacggtcag
     8281 accgaacgcc aacgccacgc cggcaaaacc aatgcctaat tccgggaagc ctgcggtcag
     8341 tacagcacta ccacagccac caaaaacaag ccagaaagta ccaaaacatt cagctgctaa
     8401 ttttctgaac atatccacct caattaaaaa ttgaccctgt gaaaaatatg gtcgttttat
     8461 agggtcgtcg taaaaaagtg acgacggaaa taatgcgcgg ctattttaaa aacgaaggcg
     8521 agtcattcac cagataaata aatccagtaa atttgattta gggcaacagc gggttgcccc
     8581 atatagtcat ttgtctgatt gacagtgtag tgcaagcaaa agatttaatc ctttaggcgt
     8641 aataaaaaat aatttatcat gctaattatt tgattttgtt gtttttgcag acttatcagc
     8701 aagagggagt ataacgcgat tattcgctca tttttcagac atttgccatg cttaaatgtg
     8761 atgtcatcac gtattagcaa ggcctttccc gttatactgc cagcgtaaag gataagtcac
     8821 atatttctgg aggggatatg attcttgagc gcgttgaaat tgtgggtttt cgcggtatca
     8881 accgtttgtc gttgatgctg gaacaaaaca acgtcctgat tggggagaac gcgtggggta
     8941 aatccagctt gctggacgcc ttaaccctgc tgctatcgcc agaatcagat ctctaccatt
     9001 ttgagcgcga cgatttctgg ttcccgccgg gagatatcaa cgggcgagaa catcatctgc
     9061 atattatttt gaccttccgc gaatcgctgc caggtcgaca tcgggttcgc cgttatcggc
     9121 cgctggaagc gtgctggacg ccatgcaccg atggctatca ccgtattttt tatcgtctgg
     9181 aaggggagag tgcggaagac ggcagcgtga tgacactgcg cagttttctc gataaagacg
     9241 gacatccgat tgatgttgag gatattaacg atcaggcaca ccatctggtg cgtttaatgc
     9301 cggtgctgcg cttgcgtgat gcccgtttta tgcgccgtat tcgtaacggc acggtgccaa
     9361 atgtccccaa tgtggaagtc accgcgcgcc agctcgattt cctcgcccgt gagttgtcct
     9421 cacatccgca aaatctctct gatggacaga ttcgtcaggg actttccgca atggtgcagt
     9481 tgcttgagca ttatttctct gagcaggggg ctggacaggc gcgatatcgt ttaatgcggc
     9541 ggcgggccag caatgagcaa cgaagctggc gctatctgga catcatcaac cggatgattg
     9601 accgacctgg agggcgctcg tatcgggtta ttttgctcgg cctgtttgct actttattgc
     9661 aggcaaaagg cacattgcga ctggataaag acgcccgtcc attgttgctg atcgaagatc
     9721 cagaaacgcg tttacacccc attatgcttt cagttgcctg gcatctgttg aatcttttgc
     9781 ctttacaacg tattgctacc accaactctg gcgagctgct ttcgttaact ccagtggaac
     9841 atgtttgccg cctggtgcgt gaatcttctc gtgttgccgc ctggcgtctg gggccaagtg
     9901 gcttgagcac cgaagacagc cgccgcatct cttttcacat tcgttttaat cgtccgtcat
     9961 cgctgtttgc tcgctgctgg ttgctggtgg aaggggaaac ggaaacctgg gttatcaatg
    10021 aactggcgcg tcagtgcgga catcattttg atgccgaagg gatcaaggtc attgagtttg
    10081 cccagtccgg gctaaagcca ctggttaaat ttgcccgccg aatggggatt gaatggcatg
    10141 tactggtcga tggcgatgaa gcagggaaga aatatgccgc tacggtacgc agcctgttga
    10201 ataatgatcg ggaagccgaa cgagaacatt taacggcgtt accggcgctg gatatggaac
    10261 attttatgta tcgccaggga ttttccgatg tgttccaccg cgtggcgcaa atcccggaaa
    10321 atgtcccgat gaatctgcgc aaaattatct cgaaagcgat ccatcgctct tccaaacccg
    10381 atcttgccat tgaagtggca atggaggccg gacgtcgtgg tgtggactcc gtaccgacgc
    10441 tgctgaaaaa aatgttctca cgcgtgctgt ggctggcgcg cggtcgcgcg gattaaccgc
    10501 gaaacatcgt ggccatttgt ggctgaatag cgtcgagcat ctcatagcgc cgacggtatt
    10561 cagcccgttt tttactggcg atttcggcaa tctcttttcg tgctatctgt gctggaaggc
    10621 ggtaatggcg ttcagcatca catacgccgc caaccgattc ccagaaagcg ttgtaatcgg
    10681 catggatctt gccttcttta tcgcgataac gcaggctgcg gtaaatatgc gtttcattgc
    10741 tgacggcaat aatctgctct acctgcaaac gttgggcaaa cagacaggcc gcttccatca
    10801 cgaggcgttt gggaaatagc ccgtggcagg ctttcgtcgc attctggatt tcctgatgtg
    10861 gaatttccca ttttgcgcct tgcagtccac caataaacat cgttcttttc ccctgatatt
    10921 cacacagggt aaacgtgatt tctgccagag gaataccttc gctgttgcgg aacaggattg
    10981 tgctgtcacc ttctttatcc attgagatca tcatggtcag ctcaagcgtg aactgctcgc
    11041 cgtttttgcc ttccagcttc gccagttgca gcccgggggt attcaaatat aagctgaatt
    11101 cttccgccga catacatcca cggagtaacg cataatggta acgtaacgcc tccagcaatt
    11161 gcttacggct aagattcgcc gcaaggtaag ggcgatgcag acgcacaggc agtcgcggct
    11221 ggcgcgttaa caatacattg agattaggcc agtgggaaag ttcgttcatc cactcaacgc
    11281 ttaaacgcgg cataatcaac gagcgcagca aaaatttctg gcgaaaacta cggcgatgcc
    11341 agaatttacc cggccgacac tgtccacgtg ccagactaag aaaaagtgac aggctgctga
    11401 gagattcaga tgacgtaaag gtccgttcgg ttagctgcga catattcatg aaatcaatgg
    11461 ttgtacatga cgtcgatttc accattgcgt atcttaacca aacatcaata gtgtgattac
    11521 taacgtaaat tttagggttt tgttgatatt tcgttgaagt taatgacccg gattggcata
    11581 tggagtattc agaatattta tgaaaaagcg gaaaaccgtg aagaagcgtt acgttattgc
    11641 gctggtgata gtcatcgccg gactgattac gttatggaga attcttaacg cacccgtgcc
    11701 gacttatcag acactgattg tgcgccccgg tgatttacag caaagcgtgc tggcgaccgg
    11761 aaagctggac gcgctgcgta aggttgacgt gggcgcgcag gtcagcggtc agttgaaaac
    11821 gctgtcggtg gcgattggcg ataaagtaaa aaaagaccag cttttagggg ttattgatcc
    11881 tgaacaggct gaaaaccaga tcaaggaggt cgaagcaacg ctgatggagc tacgcgcgca
    11941 gcggcagcag gcggaagcgg agctgaaact ggcgcgggtg acgtattccc gtcagcaacg
    12001 tctggcacaa acgcaggcgg tttcactgca ggatctcgac accgccgcga cggagatggc
    12061 tgtgaaacag gcgcaaattg gcaccattga cgcgcaaatc aagcgcaatc aggcttctct
    12121 cgatacggct aaaaccaatc tcgattacac tcgcatcgtt gccccgatgg ccggggaagt
    12181 cacgcaaatc accactctgc aaggccagac ggtgattgcc gcacaacaag caccgaacat
    12241 tctgacgctg gcagatatga gcaccatgct ggtaaaagcg caggtttctg aagcggatgt
    12301 aatccacctg aagccggggc aaaaagcctg gtttacggta cttggcgatc cactgacgcg
    12361 ctacgagggg caaatcaagg atgtactacc gacgccggaa aaggttaacg acgctatttt
    12421 ctattacgcc cgttttgaag tccccaaccc caatggtttg ctgcggctgg atatgactgc
    12481 gcaagtgcat attcagctca ccgatgtgaa aaatgtgctg acgatccctc tgtcggcgtt
    12541 aggcgatccg gttggcgata atcgttataa agtcaaattg ttgcgtaatg gtgaaacacg
    12601 cgagcgtgaa gtgacgattg gcgcacgtaa cgataccgat gttgagattg tcaaagggct
    12661 ggaagcgggc gatgaagtgg tgattggtga ggccaaacca ggagctgcac aatgacgcct
    12721 ttgctcgaat taaaggatat tcgtcgcagc tatcctgccg gtgatgagca ggttgaggtg
    12781 ctgaagggca tcaccctcga tatttatgcc ggtgagatgg tagcgattgt tggcgcttcg
    12841 ggttccggta aatcgaccct gatgaatatt ctcggctgtc tggataaggc caccagcggc
    12901 acctatcgcg tcgccggtca ggatgttgcc acgctggacg ccgatgcgct ggcgcaactg
    12961 cgccgcgagc atttcggctt tattttccag cgttaccatt tgctttcgca tttaaccgcc
    13021 gagcagaacg ttgaagtacc agccgtctat gctggtcttg agcggaaaca gcggctgctt
    13081 cgcgcccagg agttgctgca acgactgggg ctggaagacc gtacagagta ttatccggca
    13141 cagctttcgg gtggtcagca acagcgcgtt agcatcgcgc gggcattgat gaacggcggt
    13201 caggtaattc ttgccgatga accgaccggc gcactggaca gccattcagg cgaagaggtg
    13261 atggcgatcc tgcatcagct gcgcgatcgc gggcatacgg tgattatcgt cactcacgat
    13321 ccgcaggtcg ctgctcaggc cgagcgggtg atcgaaattc gcgacggcga aattgtgcgc
    13381 aatcctcccg ccattgaaaa agtgaatgtt gctggcggga cggaacctgt tgtcaacacg
    13441 gcgtctggct ggcggcagtt tgtcagcggt tttaatgagg cgctgacgat ggcatggcgg
    13501 gcgctggcag cgaataaaat gcgtacttta ctgaccatgc tggggattat aatcggtatt
    13561 gcgtcggtgg tttccattgt cgtggtgggc gacgccgcca aacaaatggt gctggcggat
    13621 attcgttcta ttggtacgaa tactattgat gtctatcccg ggaaagattt tggcgatgac
    13681 gatccgcaat atcaacaggc gctgaagtac gatgacttaa tcgccatcca aaaacaaccg
    13741 tgggtcgctt cagccacacc tgccgtctcg caaaacctgc gcctgcgtta taacaatgtt
    13801 gatgttgctg ccagtgccaa tggcgtgagc ggcgattatt ttaatgtcta tggcatgacc
    13861 ttcagtgaag gaaacacctt taaccaggaa cagctgaacg gtcgtgcgca ggtcgtggtt
    13921 ctcgacagta atactcgccg ccagcttttc ccccataaag cagatgtggt tggcgaggtg
    13981 attctggtcg gcaatatgcc cgccagagtc attggtgtgg cggaagaaaa acagtcgatg
    14041 tttggtagca gtaaagtgct gcgtgtctgg ctaccttaca gcacgatgtc cgggcgagtt
    14101 atgggccagt cgtggctgaa ctccattact gtcagggtga aagaaggatt tgacagcgcc
    14161 gaggcggaac agcaactcac gcgtttactt tcactgcgcc acggtaagaa ggatttcttt
    14221 acctggaaca tggacggcgt cttgaaaacc gttgaaaaga ccacacgtac tttacaactg
    14281 tttatgacgc tggtggcggt gatttcgctg gtagtgggcg gtattggtgt aatgaatatt
    14341 atgctggtgt cagtgaccga gcggacgcgg gaaattggca ttcgcatggc tgtaggtgcg
    14401 cgagcaagcg atgttttgca acagttcctg atcgaagccg tactggtttg cctggtcggt
    14461 ggcgcgttgg gaataacact gtcactgtta attgctttca ccttgcagct tttcttaccc
    14521 ggctgggaga ttggtttttc accgttggca ctgctgctgg cgtttctctg ctcgacggtc
    14581 accgggattt tatttggctg gttacccgca cgaaatgcgg cacgactgga tccagtagat
    14641 gctctggcac gagagtaatt tttgagataa aaatgccagc cgatcgggct ggcattttgc
    14701 ctttaggatg tacacaatga gacagaagag ctatgcgact gccgcttcta cttcgacggg
    14761 cacaataaca ctggcgtgat tgccttttgg cccctggtgg acatcaaact gaacggattg
    14821 tccagctttt agcgttctgt aaccatccat ctgaatggtg gaataatgag cgaaaatatc
    14881 ttcgccgccg ccttcagggc agatgaaacc aaaccctttg gcattgttga accacttaac
    14941 agtacccttt tccatgcttc gacatccttc gcaaatctta tacaagtaag atggaataaa
    15001 ccggggtcag agagggggct gttcaaaacc tcgccaactc tagaaataca atttagagaa
    15061 ttagggcgag ccgtcaagca tttgacaggg gacagggggc aggtatgaat caaaaatttg
    15121 aagcagttaa cgctattgac aggaatgtga cagatgtcgc tgatgccaac gatagatgat
    15181 agttatttat cagttagtgt tcgtgggtgt tcgtcatggt gtgggcatct aatttatttc
    15241 tatgattata aaggataatt tcgtttttct tgttcgccct tgttcgtcac agttcgccct
    15301 tgtacgatgc acggtgtagt caatggtgta gttaattttg atgaccggaa acacgaaaca
    15361 tgagcagggc aattcataga cttagcgaca ctcttttacg aaaattaagc ggatcaccaa
    15421 ccacaaaaag cacctttttt aatgatggtg gcaaccttag cgtaaggcat tcaaccaatg
    15481 gcctgttaac ctggtatttc acttacaggt ccggaactgg taggcagata tcacctgaac
    15541 gtctgaggct gggaaattat ccagatttga gcctgaaagc agccagggaa aaagcgtcgc
    15601 aatgtcgcgc atggctggct gaggggaaaa atccgcgcta tgaacttaat catgctgtac
    15661 aggacgcgtt agcccccgtg acggttaagg aagcgctcac ttactggctt gaatcgtacg
    15721 caaaggaaaa gcgcactgac tacgaatcac tgaaaagccg gatcaataaa cacataatta
    15781 gccagattgg agctatgcca ctggaaaaat gcgagctacg ccactggctg gcctgttttg
    15841 accagcttgc taagcgtaat ccggtatcag ccggattttt gctacaggta tgcaaacagg
    15901 cgcttaagta ctgccgaaaa cgacgatacg caatcagcaa cgttcttgat gatatggttg
    15961 tcggtgatgt tgggaaaaaa gcagaaataa gcgagcgcgt actaacaaac aaggagcttg
    16021 gggaattact acgcgccctt gatgaaaaaa tttacccacc gtactacagc gccctgattc
    16081 gcctcctgat tgtcttcgga tgtcgtacca ctgagctaag acgctctgag gtccaggaat
    16141 gggattttaa agaaatgctc tggaccgtgc cgaaagaaca cagcaaaacg aaggtagcaa
    16201 tattcaggcc aataccggaa gcgatcttgc cgtttgtcat gaagctggtg gagcagaaca
    16261 ggcacacggg cttattgctc ggagaactga aagggcaatc atccgtatca gagtacggaa
    16321 gaacggcaca cagacgcatt aatcaagccc cctggacgtt gcacgacatc cggcacacgt
    16381 ttacaacgat gctgaacgat ttaggcgtgg aaccgcacgt tgtggagcag cttaccgcgc
    16441 accagttgcc aggaatgcaa cgagtctata atcactcccg ttatcttgat gcaaaacgtg
    16501 acgctcttaa tctatgggtt gagcgtctcg agcttctcca gaacaatgat aaaaaaatcg
    16561 ttgttatgac cccgcgaatt tactcccaaa attcttgaca aattatggct gtttttcttg
    16621 cgaaaaatgg ccctctaaaa taaatcgtcg aagttcgacc aaagttcgtc gtttttttat
    16681 ttgtcaatca tcgaaaaaat tgaaattcac tctcctgaat ccttattctc tgtttcttga
    16741 ttgttccaac acctgatggt ttatctattt gtttttaatg agataattta agtgtaatac
    16801 caaatgacga catttttttg ttgcgtcgct taaaaactcg tgacacgatt ttatgaacag
    16861 cagcaaactc tagcgaactt tgacgaacat aaagttcaga aaggttacgt attcaattta
    16921 agaggtaaga aaagctaaga agtaatatct ttccgtatat ccacagcgaa acaaaaattt
    16981 ctatacgtaa ttttatgtag gactttttat gcgcgaaata aatgaagatc gtgtaatccg
    17041 cgaaaaagaa tgcagagagc taacaggcgt ttgccgcaca actcgttacg agatggaaaa
    17101 gcagggccgc ttcccctcac gtatcagtct cggcggtcgt tctgttggct gggttaagtc
    17161 tgaggttatg gaatgggtta aaagcagaaa tcgtattaat tcaggtaagg cagcgtaggg
    17221 ggaacatatg acacataaaa caaaggcgac ctgtggcggt cgccaatggg aaaacactaa
    17281 acataagcca gaacatcata gcgatttgct gtctggtggg caatgtgctc agtgtgctgg
    17341 cttgctggtg ggctattcct gctctttggc cttgcgacgc tggcggcgtt tgatttcgcc
    17401 gcgcatggct gcgtttataa actgccccgt gctttcacct tcttctttca cgttctccat
    17461 gccttcaata atttcatggg caatacgtgc agttacagat tgtgattttg cattctttgg
    17521 acccgttgcc atgtttaacc cctcatgttt ttggtgtaag acaatataca cgaaaaaaaa
    17581 atattttgaa gggttgacgt gtaagacacc gttattttac attgtgtctt acaccttgtt
    17641 gatgcaaggt acagaaaaga cgaaacccgg cagtgcgtca acactaaccg ggcttctaac
    17701 caccaacgat agcgaaagta tcgaggtagc tatgagaaat catatcacac acccgcaagg
    17761 gcgggactcg cacaacctga ataaatacat ctggcgtttt atcgccctga gcactgcaca
    17821 accgcgcgtg attaccattg aggccaccag cgaacaggaa gcacgccagc aatctcctga
    17881 tggctgcgtg atggtattcg ctgcccgtat tcgccaggga gaaattttgt gatgcataaa
    17941 atcccttttg aagtattaat ccactctgaa aacgcattaa ttaaagcgcg cgaaatgaat
    18001 gcgttgttaa ttaaattaat tgaagtgccg gaaggtggtg aggaatcagg tgtattaatg
    18061 tttgcagcca ttcagacatt actatcgcct gttattgatg aactggataa ggcaatggca
    18121 attcacgaaa ataataacgt gccccacacc ggagaataaa aataatgaaa cttaaatatt
    18181 ctggcttaac tgccagtggc aacactcacc ctaaatttac gcgcggtgat atttaccgcg
    18241 accagtacgg cggcacggta atgattaagg gcgtggcggg acggtgcgta acttaccgcc
    18301 gtgaaggtta cgaatatgat tgcgtgatgc ctgtttatca gttccggcgt gatttttctc
    18361 tggtacagac cgcgccgcat aacgtgccca ccagcaggga gaaagcacgc gccaatattc
    18421 aggaaataaa aaagatgctt aacgtattca ggggtaaaaa atgaaactgg caccgaacgt
    18481 aaaaaaacag ccacgcggaa taaaacacaa agacacagag gtaattatct ttgcgggtag
    18541 tgatgcctgg tcacacgcgg aacaatggca ggagcaggtt ggccccgcat ccggcgataa
    18601 tgtgccgcct gtgtggcttg ggccaaatca gcttgccgaa cttgatgcgc tgaaaattgt
    18661 tccggatggg aaaaagcgcg taaggctgta ccaggccgga gaactggatt tagtggagac
    18721 caaaaagatt ggtcagaagc tggcggcggc agatattcag gacgcaaatt tttaccccga
    18781 aggaatgcac gtccagaagt gtgaaaactg gcggcgctat ctgaatgctg agcgtgaaaa
    18841 tattgccgca gggcttacca tgccggagca gaaaaatacg caattggcac aaatggcaga
    18901 cagtgagcgc gcacagatgc ttgctggtcg atttgatggc gtttgtgtgc atccggaaag
    18961 tgaaatcgtt cacgtatggc gcggcggggt atggtgtccg gtcagcacaa tggaacttag
    19021 ccgcgaaatg gtggcgatct attcagagca cagggccact ttcagcaagc gcgtaatcaa
    19081 taacgccgtg gaagcgttaa aagttattgc cgaaccaatg ggcgagccga ccggcgattt
    19141 gctgccgttc gccaatggtg cgcttgacct gaaaacgggg gaattttccc cgcacacgcc
    19201 ggagaactgg atcaccacgc acaacggtat tgagtacacg caaccagcac caggggagaa
    19261 catccgcgat aacgcgccaa actttcataa atggcttgag cacgcagccg gaaaagaccc
    19321 gcgcaagatg atgcggatat gtgccgcgct gtacatgatt atggcgaacc ggtacgactg
    19381 gcagatgttt attgaggcca ccggagacgg cgggagcggt aaaagtacat tcacacacat
    19441 agccagcctt ctggcaggga aacagaacac ggtaagcgct gaaatgacat cgcttgatga
    19501 tgctggtgga cgtgcgcagg ttgtcgggag tcgtcttatc gtcctggctg accagccgaa
    19561 atatacaggc gaaggaacgg gcatcaagaa aatcacgggc ggtgaccccg tggaaattaa
    19621 cccgaaatat gaaaagcgtt ttacggcggt aatcagggcg gtggtgctgg cgaccaacaa
    19681 caacccgatg atattcaccg aacgggccgg aggtgtggca cgtcgtcgcg tgattttccg
    19741 tttcgacaat atcgtcagtg aggccgaaaa agacagggag ctaccggaaa agattgcggc
    19801 tgaaatccct gtcattatcc gccgattgct ggcgaacttt accgactctg agaaagcacg
    19861 ggctttacta ctggaacagc gtgacggtga tgaagctctg gcaataaagc aacagacgga
    19921 tccggttatt gagttttgcc agttcctgaa ttttctggag gaagcacgcg gcctgatgat
    19981 gggcggcggt ggcgattcag tgaagtacac gaccagaaac agcctttacc gcgtctatct
    20041 ggcgtttatg gcgtacgcag gcaggagcaa accgctaaac gtaaatgact ttggcaaggc
    20101 tatgaagcct gccgcaaaag tttacggaca tgaatatatt acgcggaaag ttaagggggt
    20161 aacgcagact aacgcaataa caacagacga gtgcgacgcg tttttataat tttttgtaat
    20221 tgctgtctac cctgtctacc tgagtaaaga aaaatacatt taattcagtg cattaagtta
    20281 ggtagacagc ctttttttac tgtctaccca ctatctaccc tctctacctg attttacctg
    20341 aatcagacag ggaggtagac acggggtaga tagtggataa aagcactcta ccccgctgaa
    20401 agccgtgcca ttactggcat gagggccact aaggtagata aggtagacaa ggggaggcac
    20461 aactcaaaac tttttaaacg agggggtaaa aataaatatg tacacttcag ggaaatttaa
    20521 taaatcactc aaaaaacgca gagacagaac agaaccgaaa tatcgcgcgt tagacatgac
    20581 agagcacgct ttaaaggtgg caatcagaac gatagaccgc cacgcggggg aaggatacgc
    20641 gaaagcacat cccgaactga taagcgcatt catgaccacg acggcggcaa attttgccac
    20701 gctgacagag cgggagattg cggaagctga acaggtgaca accatcaacg ttaaaaacgt
    20761 agaggtggaa tcatgacagc acagatagcg gcttacgggc ggctggtggc tgacccacag
    20821 ttaaagacca ccagcaaggg cacacaaatg acgatggcga gtatggcggt tcccctgaca
    20881 tgcagccagg ctgatgacgg aacggcgacg atgtggttat ccgtcctggc gtttggcaga
    20941 caggccgacg cactggcaaa acaccgcaaa ggcgagctgg tgagcgtggc gggtaacatg
    21001 caggtaagcc agtggacagg ccagaacggc gaaacgcggc agggctggca ggttatcgca
    21061 gacagcgtaa tcagtgcgag aacggcgcga ccgggcggca aaaaaggcca gcaggggcag
    21121 gctactgacg cactgaacag ggcaaaacaa caggcgggga atgatgatcc gtacggggat
    21181 aacataccgt tttaagcaac gagtggcaga agccggagca atccggctt
//
LOCUS       DAZFAB010000080        20452 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZFAB010000080 DAZFAB010000000
VERSION     DAZFAB010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20452
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.080"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            116..832
                     /gene="yidP"
                     /locus_tag="ACHJWA_003343"
     CDS             116..832
                     /gene="yidP"
                     /locus_tag="ACHJWA_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO4309878.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPDKS"
     gene            complement(829..2490)
                     /gene="yidE"
                     /locus_tag="ACHJWA_003344"
     CDS             complement(829..2490)
                     /gene="yidE"
                     /locus_tag="ACHJWA_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFO4309879.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(2686..3114)
                     /gene="ibpB"
                     /locus_tag="ACHJWA_003345"
     CDS             complement(2686..3114)
                     /gene="ibpB"
                     /locus_tag="ACHJWA_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFO4309880.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(3226..3639)
                     /gene="ibpA"
                     /locus_tag="ACHJWA_003346"
     CDS             complement(3226..3639)
                     /gene="ibpA"
                     /locus_tag="ACHJWA_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFO4309881.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(3633..3705)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            3945..4277
                     /gene="yidQ"
                     /locus_tag="ACHJWA_003347"
     CDS             3945..4277
                     /gene="yidQ"
                     /locus_tag="ACHJWA_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO4309882.1"
                     /translation="MIRNVLLAFMICSGMALLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(4279..5493)
                     /gene="yidR"
                     /locus_tag="ACHJWA_003348"
     CDS             complement(4279..5493)
                     /gene="yidR"
                     /locus_tag="ACHJWA_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFO4309883.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRVSQGAHVGVVTVHPKSEKYVFIHGPENPHETWHYDFHHRRGVIVE
                     GGKMNNLDAMDITVPYTSGALRGGSHVHVFSPNGEMVSFTYNDHVMHQFDSALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGIVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLGNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            5594..6658
                     /gene="cbrA"
                     /locus_tag="ACHJWA_003349"
     CDS             5594..6658
                     /gene="cbrA"
                     /locus_tag="ACHJWA_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFO4309884.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRYLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRSVLLKQPEKINAAYWHATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGMAHIPQLKD"
     gene            complement(6655..6768)
                     /locus_tag="ACHJWA_003350"
                     /pseudo
     CDS             complement(6655..6768)
                     /locus_tag="ACHJWA_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418146.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6904..7560
                     /gene="yidX"
                     /locus_tag="ACHJWA_003351"
     CDS             6904..7560
                     /gene="yidX"
                     /locus_tag="ACHJWA_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HFO4309885.1"
                     /translation="MKLNFKGFFKAASLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVANDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(7606..8418)
                     /gene="yidA"
                     /locus_tag="ACHJWA_003352"
     CDS             complement(7606..8418)
                     /gene="yidA"
                     /locus_tag="ACHJWA_003352"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFO4309886.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGNTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(8533..8931)
                     /gene="yidB"
                     /locus_tag="ACHJWA_003353"
     CDS             complement(8533..8931)
                     /gene="yidB"
                     /locus_tag="ACHJWA_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFO4309887.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQPVSGEQLESALGTNAVSDLGQKLGVDTSTASCLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            9049..9147
                     /locus_tag="ACHJWA_003354"
                     /pseudo
     CDS             9049..9147
                     /locus_tag="ACHJWA_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9171..11585)
                     /gene="gyrB"
                     /locus_tag="ACHJWA_003355"
     CDS             complement(9171..11585)
                     /gene="gyrB"
                     /locus_tag="ACHJWA_003355"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFO4309888.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(11614..12687)
                     /gene="recF"
                     /locus_tag="ACHJWA_003356"
     CDS             complement(11614..12687)
                     /gene="recF"
                     /locus_tag="ACHJWA_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFO4309889.1"
                     /translation="MSLTRLLIRDFRNIETADLALCPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(12687..13787)
                     /gene="dnaN"
                     /locus_tag="ACHJWA_003357"
     CDS             complement(12687..13787)
                     /gene="dnaN"
                     /locus_tag="ACHJWA_003357"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFO4309890.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(13792..15195)
                     /gene="dnaA"
                     /locus_tag="ACHJWA_003358"
     CDS             complement(13792..15195)
                     /gene="dnaA"
                     /locus_tag="ACHJWA_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFO4309891.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKSVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            15492..15572
                     /locus_tag="ACHJWA_003359"
     CDS             15492..15572
                     /locus_tag="ACHJWA_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4309892.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            15802..15942
                     /gene="rpmH"
                     /locus_tag="ACHJWA_003360"
     CDS             15802..15942
                     /gene="rpmH"
                     /locus_tag="ACHJWA_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFO4309893.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            15959..16318
                     /gene="rnpA"
                     /locus_tag="ACHJWA_003361"
     CDS             15959..16318
                     /gene="rnpA"
                     /locus_tag="ACHJWA_003361"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFO4309894.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVANLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            16282..16539
                     /gene="yidD"
                     /locus_tag="ACHJWA_003362"
     CDS             16282..16539
                     /gene="yidD"
                     /locus_tag="ACHJWA_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFO4309895.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFNTREH"
     gene            16542..18188
                     /gene="yidC"
                     /locus_tag="ACHJWA_003363"
     CDS             16542..18188
                     /gene="yidC"
                     /locus_tag="ACHJWA_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFO4309896.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            18294..19658
                     /gene="mnmE"
                     /locus_tag="ACHJWA_003364"
     CDS             18294..19658
                     /gene="mnmE"
                     /locus_tag="ACHJWA_003364"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFO4309897.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSAKTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            complement(19808..20452)
                     /locus_tag="ACHJWA_003365"
                     /pseudo
     CDS             complement(19808..20452)
                     /locus_tag="ACHJWA_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228226.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="toxin"
BASE COUNT         4941 a         5416 c         5193 g         4902 t
ORIGIN      
        1 tgagcactgg tacacagcat cgaacacatc tttaaaaaaa agatgttttt tcaattgatt
       61 aagcagaact tgtgggcgca ttacccgggc ttgcaggcaa aaaagagatc tagagatgat
      121 ctacaaaagc attgcggagc ggttaagaat tcgacttaat tccgcggatt tcacgctaaa
      181 cagccttctt cccggtgaaa aaaagctggc ggaagagttt gcggtatcgc ggatgaccat
      241 ccgtaaagcg attgacctgc tggtagcgtg ggggctggtg gtccgccgcc acggcagcgg
      301 cacttacctg gtgcgcaaag atgtgctgca tcaaaccgcc agcctgaccg ggctggtgga
      361 ggtgttaaaa cggcagggaa aaacagtcac cagccaggtg ctgatttttg aaatcatgcc
      421 tgcgcctccg gcaattgcca gccagttgcg gattcaaatc aacgagcaga tctacttctc
      481 ccgtcgcgtt cgttttgtgg aagggaaacc gctgatgctg gaagacagct atatgccggt
      541 aaaactgttc cgtaaccttt cgctgcaaca tctggaaggg tcgaagtttg aatatattga
      601 acaagagtgc gggattttga ttggcggtaa ttatgaaagc ctgacgccgg tgctcgccga
      661 tagactgctg gcgcggcaaa tgaaggtagc ggaacacacg ccactgctgc ggatcacctc
      721 gttgtcatat agcgagagcg gggagttttt gaattattca gtgatgttca gaaatgccag
      781 cgaataccag gtggagtacc atttacggcg actccacccg gacaagagtt aaccgatact
      841 ccagaagagc accgccagta attggggggt gataatgcgc aggaacatca ctaacggata
      901 gacagtggcg taagagagcg ccgccgcacc gctggttgga tgaagattat tagcaaacgc
      961 cagcgccgga ggatcggtca tggaacctgc cagcatcccg cacatggtca ggtaattcat
     1021 tttggctaac atccgcgcca gaatgccaac agtaatcagc ggaacggcgg tgatcagggc
     1081 accataacca atccagctta gcccttcgcc attgaccagg gtattcacaa aatccccacc
     1141 agatttcaga ccaacgaccg agaggaacag cacgatcccc agttcccgca gcgcgaggtt
     1201 ggcacttggc ggcataaacc agtacagctt gccgatactg ccgatacgcc cgaggatcaa
     1261 cgccataatc agcggaccgc ccgccagccc cagtttcaac gcggccggga atcctggcac
     1321 aaagacggga atagaaccta acaatacgcc aagcccgatg ccaataaaca ctggcagcat
     1381 ctgaacctgt tgcagttttt gctgcgcatt ccccagcaca ttggcaacgg catcaattgc
     1441 ggacggacgc cccaccaggt tgaggatatc gccgaactgc aggctgatat cgccactggc
     1501 gaccagttcg accccggcac ggttcaggcg cgagataaca acgtcatagc gttctttaaa
     1561 atgcaggtcg cgaatacgct taccaagcac gttttcattg gtgaccacca cacgctcgac
     1621 gcgcaaatca gtgcctttcg tggacagcga agtatcgacc tcctgaccaa tcaccagttg
     1681 cgcattatgt aaatccgctg gctgacccac cagatgcagc aaatcgccca gttggataat
     1741 ggtatctggc gaaggaactt ttagggtttc ttcgcgtttc agacgcgagc agataatttt
     1801 gtcgccgttg agaatcggta catctttaat ggctaaatca tgcaggttag ggttctcaac
     1861 gcgaatattg atagtcttga tcagcgcgcc gccattggtg cgtgaagact cgtgctgctg
     1921 ggcttctgtc tcgacattga cgcggaaaat aacccgcaac atccacatgg tgaacaatat
     1981 cccgcaaatg ccgaatggat acgccatcgc gtaactcatc cccatctgat cgaccatttc
     2041 cattggtgta cccaggtcgc gcaaaatctg ctgccctgcc cccagcgctg gcgtattggt
     2101 taccgcaccg gagaaaatcc ccagcactac cggcagtgga atatcaaaca gtttatgcag
     2161 gatggcggta accagaccac cgatgatgac gatcagaaca gcaaacaggt tgaggcgtaa
     2221 tccggagacg cgcaatgagg caaagaagcc cggccctacc tgaatcccga tggtataaac
     2281 gaacaggatc aggccaaatt cctgaataac atgcagcata tcgctactta atgtcatccc
     2341 cgcctgagaa acaaaatggc cgacgatgat cccaccaaac agcacgccgc caatacctaa
     2401 tcctatgccg cgaaatttga cgttgccgat aaacaaaccg acgactgcca ccaaagccaa
     2461 aatactgacc gttaatgcta tatcactcat cgtccatttc tcttgagata acattttaat
     2521 tgctacggat tatggctgaa tcggtgaagc agatttgcct tatagcgcac attatcacgg
     2581 gaaagaacgt gccgaaatat cttaaacagt cgaaatttaa cgcattgttg aagaagaaag
     2641 acaaaaaaag ccccaccaga atggcggggc aaagagaata gctagttagc tatttaacgc
     2701 gggacgttcg ctgatagcga tacgctgcgc tgcgatgggt tcaggctcat tacgaattaa
     2761 atcaatatgc agtaaaccgt ttacgaaggt tgcgccagag acttccatat tttcagccag
     2821 cgtaaagctc aggctaaatg gctgattcat aagcccttga tgcagccatt ttttctcttc
     2881 ttttggctgc tccggcgtgc cttttacgct caggcgcgta ccttccagtt gaatctctaa
     2941 atcttcctga cggaaacctg ccagcgcaag ggtaatgcgg tagtggttat cgtcgctttt
     3001 ctcaatgttg tacggcggga agctctgact ttcaccggcg ttttgcagtg cgttggccag
     3061 tttgtcaaaa ccgatccatt gacgcatcag tggggataaa tcgaagttac gcatagtcat
     3121 ttctccttct aagaagcgag taagtacctg caaatccgaa gattcgcata tgctccctga
     3181 cggcgagcat ggagatgtca ggccgcgcaa ggcggcctta ggaaattagt tgatttcgat
     3241 acggcgcggt tttttcgctt ccggaatcac gcgttcgaga tcgatataca gcaaaccatt
     3301 tactaggtta gcaccacgaa catgaatgtt ctcagctaac tggaatttgc gttcaaagtt
     3361 gcgttcagcg atgccctggt acagataggt gcgctctttt tgttcgtcgg cgtgagcacc
     3421 tttcaccacc agcagattat cctgggcggt aatttccagt tcgctctcag caaaaccagc
     3481 cacagcgata gcaatgcggt aatggttttc gtctaccagt tcaacgttat acggagggta
     3541 gccgccatta ctctggctct ggttgttttc taagtggtta aacaaacggt caaatccaat
     3601 agcagaacgg taaagcgggg ataaatcaaa gttacgcata atcaatagct cctgaaatca
     3661 gcgagaatgt aagaccttcc acaatggaca ggtcaggtag ccagaacacc catcaggcgc
     3721 gtcctcatcg gctacgatgt aaaaatgggt ctggaaatga acttttcaag ccttatcagc
     3781 ggactttttt ttgcagttta tggtctattg catagactga gggggcagca cgaaacgtta
     3841 aagtgcgata gccgccacag agcgacgtaa tgatgatgtt attttcagca agggatcgct
     3901 ataactcatc aggccaacac ccacgatcaa cagatgaatt tattatgata agaaatgtgt
     3961 tgttagcgtt catgatatgc agcggaatgg cattactcgg aggatgctcc agcgtgatgt
     4021 cccataccgg agggaaagaa ggaacgtatc ccggcacccg cgccagcgcc accatgattg
     4081 gcgatgatga aaccaactgg ggcactaagt cgctggcgat tctcgatatg ccgtttaccg
     4141 ctgtgatgga cacgctttta ttaccgtggg atgtgttccg caaagacagc tcggtgcgct
     4201 cacgcgttga aaaaagcgaa gccaacgcgc aagccaccaa tgcggttatt ccaccggcca
     4261 gaatgcctga taactgattt agcgatcagt ttcggtgatc cacagctggc cgccttccat
     4321 ccacgccagc cactgaccat ccggcgagaa gaccacggcg tccgcagaag gtggattggc
     4381 gtggttttcg gttaaatact caacctcgcc gctttgcgca tgggcacaag caattcgatt
     4441 acccagcaca aagcccaacc attctcctga cggatgccag ttaaatgcag actgaatatc
     4501 cgttttgtta tgggttaact ggcgcggctc gccgccctgt ggcgagataa gccacagttg
     4561 cacaatgccg ttatcatctc gcattaaaaa cgcgatttgc gtaccctgcg gattacagcg
     4621 cacccagtgg cgcgggacgt tgactaaccc cggataagcc cgatggtggg taaaggttaa
     4681 acgtcgctgc acgatgccac gcggtggcgc gggcagggtt gtttccgttc cacttaacgg
     4741 cgcatcacct gctgctttcc agccagcttc atcttgcggt aactcaacga taaacagctc
     4801 cggcactttc tcgccctttg gcgaaagtgt gtcgccaata aacgccagcg cgtgatttcc
     4861 tacccatcct tcttcataag cacgattgat ttcatcgctg ccaggctgcg gcgtgggcgt
     4921 ggttttactc accagcacgc accagtggct accgctgtat tcacgcggat gctgcttttg
     4981 tacgttgacc gggccaaacg gtgcagcaac cccgacgttt cgcaaatcca gcgccgaatc
     5041 gaactgatgc attacatggt cgttataggt aaaactcacc atttcaccgt tcgggctaaa
     5101 gacatgcaca tggctgccgc cacgcagcgc ccctgatgtg tacggaacgg taatatccat
     5161 tgcatcgaga ttgttcatct tgccgccttc aacaatcacg ccgcgacggt gatggaaatc
     5221 gtaatgccat gtttcatgag gattttcagg tccgtggata aagacatatt tctctgactt
     5281 tggatgaacg gtcaccacgc cgacgtgcgc gccctgtgac acgcgataga taacctcgac
     5341 ctcgccggta tggatattca cacgctcaat ggtctcgccg gtaaacgacg cgccagaagg
     5401 acgcacgtca aataccagcc actggctgtc gggcgtccag gtattggtat tggtgagcag
     5461 gtgattacgg ggagcaaagg ttatctgttt catggcgcgg tcctgataca aaacggttcc
     5521 cgccatcata cttcacaagg acttcacttt cagacaatag tcttttgcca tcgtgatgaa
     5581 tgtgagtgaa aagatggaac atttcgacgt ggcgattatt ggcctcggcc cggcagggtc
     5641 ggcgttggca cgaaagttag ccggcaaaat gcaggtgatc gcgctggata aaaagcacca
     5701 gcatggtact gaaggtttca gcaagccttg tggcggtctg ctggcaccgg acgcgcagcg
     5761 atcttttatt cgcgatggac tgacgcttcc tgtcgatgtg atcgccaatc cacagatttt
     5821 cagcgtcaaa actgtcgacg tcgccgcatc gctcacgcgt aactaccagc gaagctatat
     5881 caatattaat cgccatgctt tcgacttgtg gatgaaatcg ctgatccccg ccagcgttga
     5941 ggtttatcac gatagcctgt gccggaaaat ctggcgtgag gatgataaat ggcatgtcat
     6001 ttttcgtgca gacggttggg agcagcatat tactgcccgc tatctggtcg gtgcagatgg
     6061 tgccaactcg atggtgcggc gatatctcta cccggaccat cagattcgta aatatgtcgc
     6121 tatccagcag tggttcgcag agaaacatcc ggtgccgttc tactcatgca tctttgataa
     6181 tgcgataact gactgttact catggagtat cagcaaagac ggttatttta tctttggcgg
     6241 tgcctatcca atgaaagacg gtcagacgcg tttcacgacg ctgaaagaga aaatgagcgc
     6301 ctttcaattc cagtttggta aggcggtaaa aagcgaaaaa tgcacggtgc tatttccctc
     6361 acgctggcag gattttgtct gcggtaagga caacgccttt ctgattggtg aagcggcggg
     6421 atttatcagc gccagctcgc tggaggggat aagctatgcg ctggatagcg cagagattct
     6481 gcgttcggtg ttactgaagc agccagagaa gatcaacgca gcctactggc acgccacccg
     6541 caaactgcgt ttaaaactct tcggcaagat agtaaaaagc cgatgcctga ccgcaccggc
     6601 tttaagaaag tggattatgc gcagtggtat ggcgcatatt ccacagttga aagattagcc
     6661 aacgcgcgtc acatcgccca ccagcaggat gtaagagagc gcgccaatca acgcgacggc
     6721 ggagatataa accagtgcag gtgcgaaccc atagccttgc aacagatatt agtccacaaa
     6781 aaaacacctc ataagaaaag gtcttatatc gggcagaaat ctgaaaaggg agatcaggcg
     6841 ctaaacagga gggaaagaag agtatgcttt caacggctta gctactcgtt taaaggatta
     6901 atcatgaagt tgaattttaa gggatttttt aaggctgcca gtttattccc actggcgctg
     6961 atgctttcag gctgtatctc gtatgctctg gtttcccata ccgcaaaggg tagttcagga
     7021 aagtatcaat cgcagtcaga caccatcact gggctatcgc aggcaaaaga tagtaatgga
     7081 acaaaaggct atgtttttgt aggggaatcg ctggattacc ttatcactga tggtgccgat
     7141 gacatcgtta agatgctcaa tgatccagca cttaaccggc acaatattca ggttgccaat
     7201 gacgcaagat ttgttttaaa tgcggggaaa aagaaattta ccggcacaat atcgctttac
     7261 tactactgga ataacgaaga agaaaaggca ctggcaacgc attatggttt tgcctgtggt
     7321 gttcaacact gtaccaggtc actggaaaac ctaaaaggca caatccatga gaaaaataaa
     7381 aacatggatt actcaaaggt gatggcgttc taccatccat ttaaagtgcg attttatgaa
     7441 tactattcac ccagaggcat tccggatggt gtttccgcag cattactgcc agtgactgtt
     7501 acgctggaca tcattactgc gccgctgcaa tttctggttg tatatgcagt aaaccaataa
     7561 tcagtaagcg ggcaaacgcg tttatgctgt ttgcccgccc acagattaat tcagcacata
     7621 cttctcaata gcaaacgcca cgccatcttc aaggttggat ttggtgacaa agttcgccac
     7681 ttctttcacc gaaggaatag cattatccat tgccacaccg acgcctgcat attcaatcat
     7741 tgcgatgtcg ttttcctgat cgccaatcgc catgatttct tccggtttaa tacctaacac
     7801 gtcggccagt gatttcacac ccgtaccttt gttaacgcgt ttatcaagga tttcgaggaa
     7861 gtacggcgca cttttcagca cggtatattt ctctttcact tcctgcggaa tacgcgcgat
     7921 agcctggtcg aggatggcgg gttcatcaat catcatcact ttcaggaact gggtattggg
     7981 gtccattttc tccgcttcgc agaacaccag cggaatggtg gcaacgaagg attcatgcac
     8041 cgtgtagtag ctgatatcac ggttggcggt gtacagcgtg gtgcggtcca gggcgtggaa
     8101 atgagaaccg acttcgcgag agagtttttc caggaaacga tagtcgtcat agctgagagc
     8161 agtttgcgcc acggtgttac catcagcggc cttctgtacc agcgcgccgt tataagtaat
     8221 gcagtagtcg cccggctgtt ccatatgcag ctctttcagg taattgtgaa cacctgcata
     8281 agggcgaccc gtcgttagca cgacattcac gccacgggcg cgagctgcgg caatcgcatt
     8341 tttaacggca ggtgaaatgg tgtgatcggg cagcagaagg gtgccatcca tatcgatagc
     8401 aatgagtttg atagccatga gttccccagg tagattggtt cctgacccat gctaacgcga
     8461 ttccgctcaa aaatcagtac aacacccgag ggaaaagggg gatgcaacgc gggttgcatg
     8521 ctccccattt gcttagcgga agagtttccc tttcagcagt tccatgcctg cggaaagcag
     8581 atcgttattg gcttgtggtg acacttcacc ttgcggtgag agcgcatcaa taatcttcgg
     8641 caattgttct gccagtaaac aggaagccgt actggtatcc acgccaagtt tttgtccaag
     8701 atcggatacc gcgtttgtgc cgagtgctga ttccagttgc tcgccgctaa ctggttgatt
     8761 gccctgttga ttactcagcc aggttgagag aatggcccct aagccgccac tttgcagttt
     8821 ttccagcagc acctgaatgc caccctgctc ctcaacccaa cttaaaatcg cctgatattt
     8881 ccccgcatcg cctttcagaa aggcgccgac aacttcatca aaaagcccca tgataatcac
     8941 ctgtaaagcg ttacgtgttg acccaaaaag tatagaactg cgggatgata attgcggatt
     9001 gcagaaataa aaagggcgga gatgatctcc gcccttttct tatagcttct tgccggatgc
     9061 ggcgtgaacg ccttatccgg cctacaaaaa catgcaaatt caatatattg caggagtctc
     9121 gtaggcctga taagcgtagc gcatcaggca cgctcgcatt gttaacgcca ttaaatatca
     9181 atattcgccg ctttcagggc gttctcttca ataaacgcac ggcgcggttc aacggcgtcg
     9241 cccatcagcg tggtgaacaa ctggtcagcg gcaatcgcat ccttaacggt aacgcgcagc
     9301 atacgacggc tttccgggtc catggtggtt tcccacagct gttccgggtt catctcgccc
     9361 agacctttat aacgctggat ggagaggccg cgacgggact ctttcaccag ccagtccagc
     9421 gcctgctcga agctggctac cggctgacga cgctcgccac gttcgataaa cgcatcttct
     9481 tccagcaagc cacgcagttt ctcacccagc gtgcagatac gacgatattc gccaccagtg
     9541 ataaactcgt gatccagcgg atagtcagta tccacaccgt gggtacgcac gcgaacaatc
     9601 ggctcgaaca ggttttgttc ggcattggtg tggacatcaa acttccactg gctgccgtgc
     9661 tgttctttgt cgttcagttc gctgaccagc gcgttcaccc accgggtaac ggtctgctca
     9721 tcagagaggt cggcttccgt cagcgtcggc tgatagataa gctctttcag cattgctttc
     9781 ggataacgac gctccatgcg gttgatcatt ttctgcgtcg cgttgtactc agacaccagt
     9841 ttctctaacg cttcgccagc caatgccggt gcgctggcgt tggtgtgcag cgttgcgccg
     9901 tccagcgcga tagagatctg gtactgatcc atcgcttcgt cgtctttaat gtactgttcc
     9961 tgcttgcctt tcttcacttt gtacagcggc ggctgagcga tgtagacgtg accgcgttca
    10021 acaatttccg gcatctgacg atagaagaag gtcaacagca gcgtacgaat gtgcgagccg
    10081 tcgacgtccg catcggtcat gatgatgatg ctgtgataac gcagtttgtc cgggttgtac
    10141 tcgtcacgac cgataccaca accaagcgcg gtgataagcg tcgccacttc ctgagaagag
    10201 agcatcttat cgaagcgcgc tttctcgacg ttgaggattt tacccttcag cggcagaatc
    10261 gcctggttct tgcggttacg cccctgcttc gcagagccgc ccgcggagtc cccttccaca
    10321 aggtacagtt cggaaagcgc cggatcgcgt tcctggcagt ctgccagttt gcccggcagg
    10381 cccgctaagt cgagcgcacc tttacggcgg gtcatttcac gcgcgcgacg cgccgcttca
    10441 cgggcacggg cagcatcgat aattttgcca accacgattt tcgcgtcagt tgggttttcc
    10501 agcaggtatt ccgccaacag ttcgttcatc tgctgttcaa ccgccgattt cacctcagaa
    10561 gaaaccagtt tgtctttggt ctgggaggag aatttcgggt ccggcacttt tacggaaacg
    10621 accgcaatca ggccttcacg cgcatcgtca ccggtggcgc tgactttggc ttttttgctg
    10681 tagccttctt tgtccatgta ggcattcagg gtacgggtca tcgccgcacg gaagcctgcc
    10741 aggtgagtac cgccgtcacg ctgcggaatg ttgttggtaa agcagtagat gttttcctgg
    10801 aagccatcgt tccactgcag cgccacttca acgccgatac cgtctttttc ggtggagaag
    10861 tagaagatat ttgggtggat cggcgttttg ttcttgttca gatattcaac gaacgctttg
    10921 atgccgcctt catagtggaa gtggtcttct ttgccgtcac gcttgtcgcg cagacgaatg
    10981 gaaacgccgg agttgaggaa cgacaactca cgcagacgtt tcgccagaat ttcatattcg
    11041 aactcggtca cattggtgaa ggtttcgagg ctgggccaga aacgcaccat ggtgccggtt
    11101 ttttcagtct cgccggtaac cgccagcggt gcctgcggta caccgtgttc gtagatctga
    11161 cggtgaattt taccctcgcg ctgaataacc agctccagtt tttgcgacag ggcgtttact
    11221 accgaaacac caacgccgtg cagaccgccg gacactttat aggagttatc gtcaaattta
    11281 ccgcctgcgt gcagaacggt catgatcact tccgccgccg atacgccctc ttccgggtga
    11341 ataccggtcg gaatgccgcg cccgtcatcc tgtacagaga cagagttatc ggcgtgaatg
    11401 gtgacgataa tttctttaca gtgacccgcg agcgcttcgt cgatagcgtt atctaccacc
    11461 tcgaatacca tgtggtgcag accggtgccg tcatccgtgt cgccgatata catacccggg
    11521 cgcttacgca ccgcatccag ccctttcagg actttgatac tggaggagtc ataagaattc
    11581 gacatcaacg tttctcgctc atttatactt gggttaatcc gttattttac ccttttccac
    11641 ggtaaacatc ttcgaatttt cgtccgacat gtctataacg tgttcagcac tgatcgcgct
    11701 gacaaagacc tgcgattgcg tagcttttaa gcggctggca agcagcccgc gacgctcatc
    11761 atcaagctca gaggcaaaat catctatcag gtagagacac cgccgcccgc tttcacgggt
    11821 gaggaactct ccttgcgcca gacgtaaggc gcacatcaac agcttaagct gcccacgcga
    11881 taaggtatct tccaccggcg caccgtcggc gcgaatgcgt aagtccgctt tatgcgggcc
    11941 atgcgcggtg taggttagct ggcgatcgcg ttcaaaatta cgttccagca cctcagcata
    12001 ttctgtctct ttctcccagc cgcgctggaa agagaaagtc agagaaaact cagggagaaa
    12061 ttgcttacag gtatcagcca tatcggccgc gataccggcg ctatactccg cgcgccaggt
    12121 actgatttgc tccgccagcg ggatcagttc tttatcccac gggcgtagct gttcgtaacg
    12181 tgtcacctgg cgcagcgccg cattgcgctg cttgagcaat cgcttgagat tgctccaggc
    12241 ggtgaaaaat ccgggttcgt tgtgaaagca tccccagtcg aggaatgctc ttctgtattt
    12301 ggggccgccg ttgagtaaag taaacccttc tggcgttatc agctgcattg gcatcaggtg
    12361 cgccagttcc gcgactttat gcccgtcagt accgtcgatg cggactttgc tgtcgccctg
    12421 tttgtctttg gttaagccaa tcgccgtctc gcgctcttcg ccctgtaatc gcccgtggag
    12481 aacaaacgcc tcctgctcgt ggcgaatgac gcgaccaatc tgcaaactgc gaaacgcccg
    12541 accatggccg agcgtataga tggcttccag cacgctggtt ttgccactgc cgttggcacc
    12601 taccagaaag ttaaagccgg gacataaagc gagatccgcg gtttcaatgt tgcggaaatc
    12661 gcggatcaac aagcgggtga gggacattac agtctcattg gcatgacaac ataagccgcg
    12721 ctctggctgg ctgcatcttc aatctgcacg ctggaaaccg aatcggtcag catcatgcgg
    12781 acgttttcgc atttcagcgc gttcagaaca tccagcacat agctgacgtt gaagccgatt
    12841 tccatctccg caccgctata ggtaacgtcg aggatctctt ccgcttcttc ctgttccggg
    12901 ttgttggcgg tgattttcag ctggttttcg ctgacataca ggcgcacacc gcggaatttc
    12961 tcgttagaga gaattgccgc gcgagcaaac gcctgcttga ggagatcgca gcctgcttcc
    13021 agatgtttgt ccggattctt cggcagaacg cggcggtaat ccgggaagcg accatccacc
    13081 agtttagagg tgaagataaa gtcgccaacg tgggcgcgaa tattgttgct gccaatctgc
    13141 acacgcagcg gattatcgcc gccgtcgagc atacgcatca gttcaatcac gcctttacgc
    13201 ggcacgatca ccgaatggct tggcaaagat tgaccaattg gcattgaaca gaccgccaga
    13261 cggtggccgt cggtcgctac ggtgcgcagt tcttcacctt cggtttcaaa cagcataccg
    13321 tttaagtaat agcgaacgtc ctgatgcgcc atagaaaact gggtcgcttc aatcagacgc
    13381 ttcatcgtcg cctgcggcag ggtaaattcg acttcactct gccagtcatc gaggttcggg
    13441 aaatccgccg ctggcagggt agacagcgaa aaacggctac gcccggagcg caccagcatc
    13501 cgctcacctt ccagctgcac ggcaatttcc gcgccttcag gcagaccacg gcagatatca
    13561 aagaatttgc gcgccggaac ggtcgtcgct cctggctcgt gtggctgaac cagcgcaacg
    13621 cgtgccacca tttccatctc gagatcagta ccggtcagcg acaacgtacc gtcagcaacc
    13681 tgtaacagca gattaccgag aatcggtagc gtaggacgac cacctaacgg accgctcacc
    13741 tgttgtagcg gttttaataa atgctcacgt tctacggtaa atttcatagg tttacgatga
    13801 caatgttctg attaaatttg aaaaatcttc tttgatatcg tggctctctt cacgcagctg
    13861 ctcgatcttc cggcaggcat gaagcaccgt agtgtggtca cgaccaccaa acgcatcgcc
    13921 aatctctggc agactgtggt tagtcagctc tttcgccagc gccatcgcca tctgacgcgg
    13981 acgagccacc gagcgggatc gacgcttgga aaggagatcc gcgactttga tcttgtagta
    14041 ctccgccacc gtcttctgaa tattgtcgat ggtgaccagt ttttcctgca atgccagcaa
    14101 gtcgcgcagc gcctcacgca cgaagtcgat agtgatcgcc cgtccggtaa agttggcgtt
    14161 ggcaatgacg cggttcagcg cgccttccag ctcacgtacg ttagatcgta gacgcttggc
    14221 gataaagaac gccacttcgc ctggcaaacg aatgtcattt tcgtcggcct ttttcatcag
    14281 gatcgccacg cgggtttcta actctggcgg ttcgatcgcc acagtcagcc cccagccgaa
    14341 gcgggatttc aaacgatcct caacgccgtt gatctctttc ggatagcgat ccgaggtgag
    14401 aatgatctgt tgattacctt ccagcagggc gttgaaggtg tggaaaaact cttcctgaga
    14461 tcgttcttta ttagcaaaaa actgaatatc gtcgatcagc agtgcatcta cggaacggta
    14521 gtagcgttta aactcttcga tcgcgttgtt ttgcagggct ttaaccatgt cctgaacaaa
    14581 gcgctcggag tgcatataaa ccactttggc attcggcttg cgcgccataa tgccgttacc
    14641 caccgcatgc agcaggtgag ttttacccag acccgtgccg ccataaagga acaacgggtt
    14701 ataggcaccg ccagggttat ctgccacctg gcgagccgcc gcgcgcgcca gttggttaga
    14761 tttaccttca acgaagttat caaacgtgtg tttgacgttt acgttagaac gataggtcgg
    14821 ttctgccggg gccgggacgt tatcccaacc tgagcgcgta gaaggcgcag cacgttgcgg
    14881 ctgcgtttgc gccacctgtg caggggccgc gacgttgctc gtcactgccg cttgtggcgt
    14941 ttgcgtcacc gatttggtgc cgacttcaaa acgcagctgt ggggcatccg ctccgcagaa
    15001 actggttagc agtccattga tattattaag gtacttgtcc cgtacccaat cgaggacaaa
    15061 acggtttggc gcgtacaggg ccagcgtgtt atcgctcagt tccgcctgca atgggcgtat
    15121 ccacatactg aattctgtgg ctggtaactc atcctgcaat cgggcaagac actgctgcca
    15181 aagcgaaagt gacacggcgg actccactcg aacaaaagtc gataatgact aaggctgaaa
    15241 cattcatgat tgttgacgta cgtcgaaaag accctgcttg agggtgacgc acgaaccgct
    15301 gtctgcggtt atatgcccga tcaagatcct gcaaaacgat cgggaccgcg gatcatagcc
    15361 taaactgcgc aagagatctt ctgtttctca cagattttcc cgatttatcc acaggatgat
    15421 ccagaactcg ctaagtgtaa acgatcctgc cgcgaggcgg gcacgattta cgccgcatat
    15481 tggaaaattt aatgaccata gacaaaaatt ggcttaatcg atctaataaa gatccaggac
    15541 gatccttgcg ctttacccat cagcccgtat aatcctccac ccggcgcgcc atgctggttt
    15601 tcactggtgt gaggccgtac gttttccctg cgaaaaggtg cggaaaagcg cggtaaataa
    15661 ggaaagagaa ttgactccgg agtgtacaat tattacaatc cggcctcttt aatcacccat
    15721 ggcttcggtg tccatcgttt catttttcgg cggatatcca ataaagccat tgaatttatt
    15781 caagtttagg tagaaatcgc catgaaacgc acttttcaac cgtctgtact gaagcgcaac
    15841 cgttctcacg gcttccgtgc tcgtatggct actaaaaatg gtcgtcaggt tctggcacgt
    15901 cgtcgtgcta aaggccgcgc tcgtctgacc gtttctaagt aataaagcta acccctgagt
    15961 ggttaagctc gcatttccca gggagttacg cttgttaact cccagtcaat tcacattcgt
    16021 cttccagcag ccacaacggg ctggcacgcc gcaaattacc attctcggcc gcctgaattc
    16081 gctggggcat ccccgtatcg gtcttacagt cgccaagaaa aacgttcgac gcgcccatga
    16141 acgcaatcgg attaaacgtc tgacgcgtga aagcttccgt ctgcgccaac atgaactccc
    16201 ggctatggat ttcgtggtgg tggcgaaaaa aggggttgcc aacctcgata accgtgctct
    16261 ctcggaagcg ttggaaaaat tatggcgccg ccactgtcgc ctggctcgcg ggtcctgata
    16321 gccctcattc gggtctatca acgcctgatt agtccgctac tcgggccgca ttgtcgtttc
    16381 actccaacct gttcaagcta cggaattgag gcattgcgca ggtttggagt gataaaaggc
    16441 agttggttga cggtgaaacg cgtattaaaa tgccaccctt tacaccctgg tggtgacgat
    16501 cccgtcccgc ccggaccatt taataccaga gaacactaac gatggattcg caacgcaatc
    16561 ttttagtcat cgctttgctg ttcgtgtctt tcatgatctg gcaagcctgg gagcaggata
    16621 aaaacccgca acctcaggcc caacagacca cgcagacaac gaccaccgca gcgggtagcg
    16681 ccgccgacca gggcgtaccg gccagtggcc aggggaaact gatctcggtt aagaccgacg
    16741 tgcttgatct gaccatcaac acccgtggtg gtgatgttga gcaagctctg ctgcctgctt
    16801 acccgaaaga gctgaactct acccagccgt tccagctgct ggaaacttca ccgcagttta
    16861 tttatcaggc acagagcggt ctgaccggtc gtgatggccc agataacccg gctaacggcc
    16921 cgcgtccgct gtataacgtt gaaaaagacg cttatgtgct ggctgaaggt caaaacgaac
    16981 tgcaggtgcc gatgacgtat accgacgcgg caggcaacac gtttaccaaa acgtttgtcc
    17041 tgaaacgggg tgattacgct gtcaacgtca actacaacgt gcagaacgct ggcgagaaac
    17101 cgctggaaat ctccaccttt ggtcagttga agcaatccat cactctgcca ccgcatctcg
    17161 ataccggaag cagcaacttc gcactgcaca ccttccgtgg cgcggcgtac tccacgcctg
    17221 acgagaagta cgagaaatac aagttcgata ccattgccga taacgaaaac ctgaacatct
    17281 cttcgaaagg tggttgggtg gcaatgctgc aacagtattt cgcgacggcg tggatcccgc
    17341 ataacgacgg taccaacaac ttctatactg ctaatctggg taacggcatc gccgctatcg
    17401 gctataaatc tcagccggta ctggttcagc ctggtcagac tggcgcgatg aacagcaccc
    17461 tgtgggttgg cccggaaatc caggacaaaa tggcagctgt tgctccgcac ctggatctga
    17521 ccgttgatta cggttggttg tggttcatct ctcagccgct gttcaaactg ctgaaatgga
    17581 tccatagctt tgtgggtaac tggggcttct ccattatcat catcaccttt atcgttcgtg
    17641 gcatcatgta cccgctgacc aaagcgcagt acacctccat ggcgaagatg cgtatgctgc
    17701 agccgaagat tcaggcaatg cgtgagcgtc tgggcgatga caaacagcgt atcagccagg
    17761 aaatgatggc gctgtacaaa gctgagaagg ttaacccgct gggcggctgc ttcccgctgc
    17821 tgatccagat gccaatcttc ctggcgttgt actacatgct gatgggttcc gttgaactgc
    17881 gtcaggcacc gtttgcactg tggatccacg acctgtctgc acaggacccg tactacatcc
    17941 tgccgatcct gatgggcgta acgatgttct tcattcagaa gatgtcgccg accactgtga
    18001 ccgacccgat gcagcagaag atcatgacct ttatgccggt catcttcacc gtgttcttcc
    18061 tgtggttccc gtcaggtctg gtgctgtact atatcgtcag caacctggta accattattc
    18121 agcagcagct gatttaccgt ggtctggaaa aacgtggcct gcatagccgc gagaagaaaa
    18181 aatcctgatt cggtgagttt tcgctaaaat aagggcggtc agttgaccgc cttttttctt
    18241 ttcgtagggc ggataagcac cgcgtatccg ccacacaaag caacaggaac atcatgagcg
    18301 ataatgacac tatcgtagcc caggccacgc ctccgggacg tggcggcgtt ggcatcctgc
    18361 gcatctccgg cttcaaagcc cgtgaagttg ccgaaaccgt gctgggtaaa ctgcctaagc
    18421 cgcgctacgc cgattatctt ccgtttaaag acgccgatgg cagcgtgctc gatcagggga
    18481 ttgcgctatg gttccctggc ccgaactcgt tcaccggcga agatgtgctg gaactgcagg
    18541 gtcatggcgg tccggtgatt ctcgacctgc tgttaaaacg cattctgacc attcccggcc
    18601 tgcggattgc tcgccctggt gagttttccg aacgcgcgtt tcttaacgat aaactcgact
    18661 tagcccaggc cgaggcgatt gccgatctta tcgatgccag ttcggaacag gcagcccgtt
    18721 cggcacttaa ctcgctgcaa ggcgcattct ccgcacgggt caaccatctg gtggaagccc
    18781 tcacccactt gcgcatttac gtcgaagcgg caattgattt ccccgatgaa gagatcgatt
    18841 tcctctccga cggaaaaatt gaagcccagc ttaatgacgt gattgccgat ctcgatgcag
    18901 tgcgtgctga agcacgtcag ggtagtttgt tgcgcgaagg gatgaaggtg gtgattgccg
    18961 gacgtcctaa tgccggtaaa tcgagcctgt taaacgcgct ggctggccgt gaagcagcaa
    19021 tcgtaaccga tatcgccgga accacgcgtg acgtgttgcg tgagcatatc cacattgacg
    19081 gaatgccgct gcatatcatc gacaccgccg ggctacgtga agccagcgat gaagtggaac
    19141 gtattggtat cgagcgcgcg tggcaagaaa ttgaacaagc cgaccgcgtg ctgtttatgg
    19201 tcgatggcac cacgacagac gccgttgatc cggcggagat ctggccggaa tttatcgccc
    19261 gtctgcccgc gaaactgccg atcaccgtgg tacgcaataa agccgatatc actggcgaaa
    19321 cgctgggaat gagtgaagtg aacggtcatg cgttgattcg tctctcggca aagactggcg
    19381 aaggcgtgga cgtgctgcgt aaccatctca aacagagcat gggctttgac accaacatgg
    19441 aaggcggctt cctggcgcgt cgtcgccacc ttcaggcgct ggaacaggca gcggagcatt
    19501 tgcagcaagg taaagcgcaa ctgttgggtg cctgggcggg cgaactgctg gcggaagagt
    19561 tgcgcctggc acagcagaac ttaagcgaaa tcaccgggga atttacctct gatgatttgc
    19621 tggggcggat tttctccagc ttctgtattg gtaagtaagt cgtctaaatt gatataaagg
    19681 gcactgttcc gttctggggg aaactaacta tcagaacgga acagcgcttg tttttttatg
    19741 agcatcctgc accaaaactg cctttactac tggctggagc gactttcaac atcttgcagt
    19801 cctgtcttta cagagaacga tattgcttta gccgattaag ctcctcttgc ggaaagttaa
    19861 attcgtctat tgctttccag aatagttctt taaatacagg attgccattt gccttgctta
    19921 ttacatattt gccttcatta ttcttcgctt ccagttgcat catcacatct ttaagataat
    19981 tttcattttc accaaccaca attttgaata ttttcagcca gagacccata tatatcatcc
    20041 gctttctcta ccgcaagaga gaatgacttc ccatcaaagt cacgggtaaa ctgctccaaa
    20101 atctcatcga aagtgaaccc gtgttgcaac gccaggcgaa tcgccgcatt cagatataca
    20161 tcaatgacct gcttactcat tgtcttatag ctggcaacat acaaagagca aactgcatta
    20221 tttctgctca gcgacaggag ttctctcact acatctttat cattttcata atggctggct
    20281 atcacttcag ctgtctccat ccaggcagca acaatcttcg tatgcccttt cactatcgca
    20341 gaatataagc ctgatgcctt cgtccatttg tctgatgctc ccgccacttt ttctcttgct
    20401 gccatcagtt tcaatatatc ttctttattt aattcatttt taaacatttt gg
//
LOCUS       DAZFAB010000081        20343 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZFAB010000081 DAZFAB010000000
VERSION     DAZFAB010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20343
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.081"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(138..884)
                     /gene="cutC"
                     /locus_tag="ACHJWA_003366"
     CDS             complement(138..884)
                     /gene="cutC"
                     /locus_tag="ACHJWA_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFO4309898.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MSRMEKIMAAAGPLAVTFHRAFDMCANPLNTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(898..1464)
                     /gene="yecM"
                     /locus_tag="ACHJWA_003367"
     CDS             complement(898..1464)
                     /gene="yecM"
                     /locus_tag="ACHJWA_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO4309899.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPR
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1680..3413
                     /gene="argS"
                     /locus_tag="ACHJWA_003368"
     CDS             1680..3413
                     /gene="argS"
                     /locus_tag="ACHJWA_003368"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFO4309900.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTHDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEIDEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3455..3499)
                     /locus_tag="ACHJWA_003369"
     CDS             complement(3455..3499)
                     /locus_tag="ACHJWA_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4309901.1"
                     /translation="MPIFRIHLDGNKKA"
     gene            3590..4078
                     /gene="yecT"
                     /locus_tag="ACHJWA_003370"
     CDS             3590..4078
                     /gene="yecT"
                     /locus_tag="ACHJWA_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFO4309902.1"
                     /translation="MFKFLVLTLGIISCQVYAEDTLIVNDHDISAIKDCWQKNSGDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKPDTEQLNLLKKSQRTWL
                     DFRDKECELILSNEDVQDLSNPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(4198..4590)
                     /gene="flhe"
                     /locus_tag="ACHJWA_003371"
     CDS             complement(4198..4590)
                     /gene="flhe"
                     /locus_tag="ACHJWA_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFO4309903.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELDGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPSLKVQRNEVIVNYR"
     gene            complement(4590..6668)
                     /gene="flhA"
                     /locus_tag="ACHJWA_003372"
     CDS             complement(4590..6668)
                     /gene="flhA"
                     /locus_tag="ACHJWA_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFO4309904.1"
                     /translation="MSNLAAMLRLPTNLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNAVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(6661..7809)
                     /gene="flhB"
                     /locus_tag="ACHJWA_003373"
     CDS             complement(6661..7809)
                     /gene="flhB"
                     /locus_tag="ACHJWA_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFO4309905.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIIKDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPHMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHVEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(8011..8655)
                     /gene="cheZ"
                     /locus_tag="ACHJWA_003374"
     CDS             complement(8011..8655)
                     /gene="cheZ"
                     /locus_tag="ACHJWA_003374"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFO4309906.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRKFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(8666..9055)
                     /gene="cheY"
                     /locus_tag="ACHJWA_003375"
     CDS             complement(8666..9055)
                     /gene="cheY"
                     /locus_tag="ACHJWA_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFO4309907.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(9070..10119)
                     /gene="cheB"
                     /locus_tag="ACHJWA_003376"
     CDS             complement(9070..10119)
                     /gene="cheB"
                     /locus_tag="ACHJWA_003376"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFO4309908.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLNQVS
                     QQMLAKISAGQAIRI"
     gene            complement(10122..10982)
                     /gene="cheR"
                     /locus_tag="ACHJWA_003377"
     CDS             complement(10122..10982)
                     /gene="cheR"
                     /locus_tag="ACHJWA_003377"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFO4309909.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFASLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(11001..12602)
                     /gene="tap"
                     /locus_tag="ACHJWA_003378"
     CDS             complement(11001..12602)
                     /gene="tap"
                     /locus_tag="ACHJWA_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFO4309910.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTTLNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEIIHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(12648..14309)
                     /gene="tar"
                     /locus_tag="ACHJWA_003379"
     CDS             complement(12648..14309)
                     /gene="tar"
                     /locus_tag="ACHJWA_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFO4309911.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVDAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDHVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(14452..14955)
                     /gene="cheW"
                     /locus_tag="ACHJWA_003380"
     CDS             complement(14452..14955)
                     /gene="cheW"
                     /locus_tag="ACHJWA_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFO4309912.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(14976..16940)
                     /gene="cheA"
                     /locus_tag="ACHJWA_003381"
     CDS             complement(14976..16940)
                     /gene="cheA"
                     /locus_tag="ACHJWA_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFO4309913.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKASEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(16945..17871)
                     /gene="motB"
                     /locus_tag="ACHJWA_003382"
     CDS             complement(16945..17871)
                     /gene="motB"
                     /locus_tag="ACHJWA_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFO4309914.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVCVPTMPSAEPR"
     gene            complement(17868..18755)
                     /gene="motA"
                     /locus_tag="ACHJWA_003383"
     CDS             complement(17868..18755)
                     /gene="motA"
                     /locus_tag="ACHJWA_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFO4309915.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRIIADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(18882..19460)
                     /gene="flhC"
                     /locus_tag="ACHJWA_003384"
     CDS             complement(18882..19460)
                     /gene="flhC"
                     /locus_tag="ACHJWA_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFO4309916.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(19463..19813)
                     /gene="flhD"
                     /locus_tag="ACHJWA_003385"
     CDS             complement(19463..19813)
                     /gene="flhD"
                     /locus_tag="ACHJWA_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFO4309917.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITLLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         4908 a         5584 c         4933 g         4918 t
ORIGIN      
        1 cactgtcacc cgctcaacag atcgcggtaa tcgttcattt tttatactat tcagggataa
       61 aaaaaggccc ctgttgaaat tgcaggggcc tggtacgagc aagcatcata ttgggcgaca
      121 tgatgcaacg gtaaaaatca tttggcctga tggcgttcaa tgattccttt catttcagca
      181 accgccgccc cgtctacgac ataacgcgaa tactcgtccg cgtgtgcatc tgatgacatg
      241 gacaatcctt gattacgata acgcatcggt gaggcttgcc acgctcccgc ggagctatgg
      301 acttccagca ctccggcatc gaggaagtgg tgcaggtttt ctgcacggac tcctgctccg
      361 gccataatga ttggagcatc acgatgggca ataagttcca taatttttga taaaccttgc
      421 agtgcgtctg atttttgccc tgatgtcagt acccgggcaa tgcccaattc cgcaagatta
      481 ttgagtgtat ttagagggtt agcgcacata tcaaaggcgc gatgaaaagt cactgccagc
      541 ggaccggcag cagccattat tttttccatt cgtgacatat cgacattacc gtcaacatcg
      601 agcacgcccg tcacgagtcc aggaaaacct aattcgcgga ccgtgcgcac atcctcaaga
      661 atggcggcaa actcaccgtc gctgtagcaa aaatcaccac cgcgtgggcg aattatcgga
      721 tgcacaggga tcgtcacccg ctggcgcacg gatttcagta cacccaacga cggcgttaag
      781 cccccctctt ttggggctgc gcataattca actctgtctg cgccgttttg ctgcgccgtt
      841 agcgcgcatt ccatgctgta acagcaaatt tccagtaatg ccatttttgc tccttaatta
      901 cgccgactgc tcgctggcaa caatctcttc aatggaccac ggatgaaatt taatggtcgt
      961 tttgccatcg gtgaccgcca gcgtcggatt cggtaatcgt tcatgctcac cttttggcga
     1021 actggtttta actgagatcc ccggtaggct gagtccttca tcagaaagca atgccagcgc
     1081 acgggcattc agcgtttctg gatcacccgg caaaacgatt tcaatatgtt cccaaccttc
     1141 gtgcgggtaa cgtttttccc taggccacgg tagctccaca atagaaaact gccagtgtgc
     1201 aacctgtacc ggttcatgca atttaaacag acaaatcggt ctgccattga tcatattttc
     1261 tgacaaaagc tcgccacact gttcaaaccc gcgacgccag cgctcagcag tggcgttttg
     1321 atggcaacgc aaagaaatgt gatcggcagt cagcggagtg atattcaacc ccagacggcg
     1381 ggaaagttca tctaatgcgt gggtaaatcg cggtaaatcc gatgcaatat cctgcagctc
     1441 gtcgatagat tgccagttcg ccataatcac tcttcgtctt tcagtaaaag cgttaattta
     1501 ccctgttgcc ctgcgccaac caaccgctga tttcacgccg cttctgatgc aatagtgaaa
     1561 acggcaatac gccacgcgca cgttgctgac gaaaacagcc atttgcagta tactcccgcc
     1621 ctaatttctt taactggtgc gggcaatttt tgctcgcttc atcaatgtaa ggtattccgg
     1681 tgaatattca ggctcttctc tcagaaaaag tccgtcaggc catgattgcg gcaggcgcgc
     1741 ctgcggattg cgaaccgcag gttcgtcagt cagcaaaagt tcagttcggc gactatcagg
     1801 ctaacggcat gatggcagtt gctaaaaaac tgggtatggc accgcgacaa ttagcagagc
     1861 aggtgctgac tcatctggat cttaacggta tcgccagcaa agttgagatc gccggtccag
     1921 gctttatcaa cattttcctt gatccggcat tcctggctga acatgttcag caggcgctgg
     1981 cgtccgatcg tctcggtgtt gctacgccag aaaaacagac cattgtggtt gactactctg
     2041 cgccaaacgt ggcgaaagag atgcacgtcg gtcacctgcg ctctaccatt attggtgacg
     2101 cagcagtgcg tactctggag ttcctcggtc acaaagtgat tcgcgcaaac cacgtcggcg
     2161 actggggcac tcagttcggt atgctgattg catggctgga aaagcagcag caggaaaacg
     2221 ccggtgaaat ggagctggct gaccttgaag gtttctaccg cgatgcgaaa aagcattacg
     2281 acgaagatga agagtttgcc gagcgtgcac gtaactacgt ggtaaaactg caaagcggtg
     2341 acgaatattt ccgcgagatg tggcgcaaac tggtcgacat caccatgacg cagaaccaga
     2401 tcacttacga tcgtctcaac gtgacgctga cccatgatga cgtgatgggc gaaagcctct
     2461 acaacccaat gctgccagga attgtggcgg atctcaaagc caaaggtctg gcagtagaaa
     2521 gcgaaggggc gaccgtcgta ttccttgatg agtttaaaaa caaggaaggc gaaccgatgg
     2581 gcgtgatcat tcagaagaaa gatggcggct atctctacac caccactgat atcgcctgtg
     2641 cgaaatatcg ttatgaaaca ctgcatgccg atcgcgtgct gtattacatc gactcccgtc
     2701 agcatcaaca cctgatgcag gcatgggcta tcgtccgtaa agcaggctat gtaccggaat
     2761 ccgtaccgct ggaacaccac atgttcggca tgatgctggg taaagacggc aaaccgttca
     2821 aaacccgcgc gggtggtaca gtgaaactgg ccgatctgct ggatgaagcc ctggaacgtg
     2881 cacgccgtct ggtggcagaa aagaacccgg atatgccagc cgacgagctg gaaaaactgg
     2941 ctaacgcggt tggtattggt gcggtgaaat atgcggatct ctccaaaaac cgcaccacgg
     3001 actacatctt cgactgggac aacatgctgg cgtttgaggg taataccgcg ccatacatgc
     3061 agtatgcata cacgcgtgta ttgtccgtgt tccgtaaagc agaaattgac gaagagcaac
     3121 tggctgcagc tccggttatc atccgtgaag atcgtgaagc gcaactggca gctcgcctgc
     3181 tgcagtttga agaaaccctc accgtggttg cccgtgaagg cacgccgcat gtaatgtgtg
     3241 cttacctgta cgatctggcc ggtctgttct ctggcttcta cgagcactgc ccgatcctca
     3301 gcgcagaaaa cgaagaagtg cgtaacagcc gtctgaaact ggcacaactg acagcgaaga
     3361 cgctgaagct gggtctggat acgctgggta ttgagactgt agagcgtatg taatcgattt
     3421 ttcgtgagag tgaagcctga tcaggtttag ccgatcaggc ttttttatta ccatctaaat
     3481 gtattctgaa aataggcatg ccattgtttt ctcgctgttg agtaagaggc cagaagcgta
     3541 atatccagct ccagggaaac ggtaacggtt caatttaagg aataccgcag tgtttaaatt
     3601 tcttgtatta acattaggca ttatctcttg ccaggtttac gcagaagata cgcttatagt
     3661 aaatgatcat gacatttcag ccatcaaaga ttgttggcaa aaaaattcag gtgatgatac
     3721 tgacattaac gtgatcaaat catgtctgcg acaagaatac aatctcgtcg atgcgcaatt
     3781 aaataaagct tatggtgaag cttatcgtta tatagaacaa gtgccacgca caggtgcaaa
     3841 aaaacctgat actgaacaac ttaacttgct taaaaaatca caacgaacct ggctggattt
     3901 tagggacaaa gaatgtgaat taatcctttc aaatgaggac gttcaggatt taagtaaccc
     3961 ttattctgaa tcagaatggc tatcatgtat gatcatacag accaatacgc gaactcgcca
     4021 gttgcagcta taccgtaact ctgaagattt ttatccaagc cctttgacaa gaggataatt
     4081 cacatatttt tggcatgttt tgttgcaagc tattcctgat aaatgattgc aacaaaacat
     4141 ccagcctttt tcactgagtt attaaacata ctcgcgagcg cgtaattttt ttgtccttca
     4201 gcgataattc acaatcactt cattacgttg tactttcagc gacggaatta accgcccacc
     4261 gccaggcact tcccagataa agcgcaacgg ttctgctgcc gctataccgg aaaaggccac
     4321 ggtggttccg ctctgcccat ctaattcgac acaacgagat tgcgaacaca agcgaacccg
     4381 cagtcctgca ggtgtcgggc cgataagctg ataccgccac gccaccagcg tcatcaatcc
     4441 tgaagcaggt tgtcgcgtag aaagaggtgc agacgacatc gactcaccgc gatgatttag
     4501 cgtaattccg acactacttg cctgccacat cccctccccg gcggcttgca ccagcagcgg
     4561 aaacaataat attgctaata aggttctcat catttaccgc caattgtcgc cgtcatgcgg
     4621 atatgtcggt tatcagacag ttccagattc gacagcacca ctaactgcgg caagctgcgg
     4681 cgcagaaagc gagacaataa tggtcgcagc gcgtggttca ccaataaaac tggcggcgca
     4741 cccaacatct cctgacggga tagcgcttcc tgagtttgcg ccagtaaacg atccgccagc
     4801 cctggctcca gtccgccccc gccctgcaac gcctgtagca acaaacgttc cagcggtgta
     4861 tcgaggccaa taacatggac ttcatctttg ccaggaaacc actgctgggt aatcgcccgt
     4921 cccaacgcca cgcgcacgac ggcggttaat tcatgtggat cgctttggat gggcgcatgt
     4981 tccgccagcg tttcgagaat agtgcgcata tcgcgaatcg gtactttttc atcgaggaga
     5041 ttttgcagca ctttatgcag tgtggtgagc gtgacgacgc cagggacgag atcttccgtc
     5101 agctttggca tctcctgggc gacgcgatcc agcagctgtt gcgcctcctg acgaccaaac
     5161 agctctgcgg catgctggct aatgaggtgg ttaagatgcg ttgctaccac cgtgctggcc
     5221 tcaaccactg tgtacccctg aatctgcgcc tgttctttta gcgcactttc aatccagata
     5281 gcattcaggc caaatgccgg atcgacggtc gcctcaccag gtaacgtccc ggcagcggtt
     5341 ccagggttaa tagccagcca gcgccccgga taagcatcac cactgcctat ctccacgcct
     5401 ttcatcaaaa tgcgatagcg ggcaggttgc agatccatat tgtcgcgaat gtgcaccact
     5461 ggcggcagaa atcccatctc ctgggcaaat ttcttgcgga tactgcgtat acggcccaac
     5521 aactcaccat cctgctggaa atcgaccatc gggatcagtc gataacccac ttccattccc
     5581 agagaatctt ccagttgtac atcgttccac gtcgcttcga caacggcatt attctctgcc
     5641 atttttaccg gttttggttc ggcaggcgct ttttgttcgc gtccacggat ccaccaggcc
     5701 agccccagca atccggcagt gaacagcaaa aataccaggt tcggcattcc aggcaccagg
     5761 ccgagtaaac cgagcacggc agcgcttaac aacataacgc ttgggttact gaaaagctga
     5821 ttcaccatct gctcgccaac atcctgatcg gtgctgacac gcgtaacaat gacccccgcg
     5881 gcggtagaaa tcaccagagc cgggatttgt gccaccagac cgtcaccaat ggtcaagagc
     5941 gtataacttt ccgccgcgtg tcccatgctc atgccatgtt gcagcacgcc gaccagcaac
     6001 ccgccgacaa cgttaatgac catgatgagg atcccggcga tggcatcgcc gcgaacaaac
     6061 ttacttgccc cgtccattga gccgtaaaaa tcggcttcct gagtgacttc ggagcggcgt
     6121 tttttcgcct catcttcacc aatcaatccg gcgttaaggt cggcgtcaat cgccatctgc
     6181 ttacccggca taccatcgag aacaaagcgc gcacccactt ctgcgatacg cccggcacct
     6241 ttggtaatga ccataaagtt gatgatcacg agaatgacaa acaccacgat accgatagcg
     6301 aaattgccac caacgaggaa gtgaccgaac gcttcaacca ccttccctgc cgccgctgcg
     6361 ccggtatgcc cttccattaa aatgatacgg gttgaagcca cgttaagtgc cagacgcaac
     6421 agcgtggtaa acaacaaaat ggtcggaaac gcagcaaact caagcgtgcg ctgggtaaac
     6481 atcgccacca gcaacaccat gatcgacaag gcaatattga aggtaaacaa caggtcgagt
     6541 atgaatgcgg gcagtggcag caccatcatc gacaagatca acaggatcaa aatcggtccg
     6601 gcaaggatct gccattgtgt cgatttcagg tttgtgggca ggcgcagcat cgcggccaga
     6661 ttactcatgg gtcggtttct cgttaataaa atccagggct tccggcaccg gaagatgagt
     6721 aggttgtaca gggcgctgtc caccagccag acgccagcgt ttcagttgcc agacccaggc
     6781 cagcacttcc gccaccgcgg cgtacagttg acccgggatt tgttgaccaa tctccacatg
     6841 tcgatacagc gctcgcgcca gcggcggcgc ttcaagcgtc gggacgttat tttcagcgcc
     6901 aatttcacga atgcgcagcg cgaccagccc tgcaccttta gcgaccactt tcggtgcgct
     6961 cattttgttt tcgtcatact gcaacgctac cgaatagtgg gtcgggttat tgacaatgac
     7021 atccgctttc ggcacatcgg ccatcatccg acgccgtgcg gcagctcgct gcatctgacg
     7081 gatccgccct ttaacatggg ggtcgccttc gctttgtttg aactcatcac gaatatcctg
     7141 ccgcgacatt cgcagctttt tcaggtggct gaagatttgg aaaaagacgt caaatcccac
     7201 cattggaatg acaccaagca ccaccagcag tgcgcatagc cctaccaaat ccatcgcatt
     7261 acccatggcg gtaatcggag actcggccat taagcgcatc atatgcggcc agtgatgcca
     7321 gagaaaaaac cccgtcacgc tgccaaccag gatggttttc aaaattgctt taagcaactc
     7381 cgcgccagtc tgtgccgaga acatccgttt tatgcccggt agcgggttga gtttggaaaa
     7441 cttcggctgc aaggattttc cgctaaatac cagccccccc agcataaccg gagaaatgag
     7501 cgccaccagc accacgccgc taatcagcgg tagcagcgcc agcatggctt ctctgatcag
     7561 cagaataatt tgcccgagga tcagattcgg gtctttgata atactgtgat caaaatgcag
     7621 cccagcggag agcatgcccg acaatcgacg ggccagcgac acaccgccaa accagataac
     7681 actaacgccc accagcaaaa tcagcagtga ggtcagttca cgggaacgcg ggatttgccc
     7741 ctcttcccgc gctttttcta gtcggtgagg tgtgggggct tctgttttgt cgtcgctctc
     7801 gtcagacacg tcgccaatcc tggaaagagt attaagcgta aatgatgcca gagcgcaaag
     7861 cgttcaatgg tttgagtaag gggcaaaaca ggcgggattt agggcttttg ctgccacaca
     7921 tcaagcatag tgtgcgtttg tcagatgcgg cgtcatcgcc ttatcagacc gcctgatatg
     7981 acgtggtcac gccacatcag gcaatacaaa tcaaaatcca agactatcca acaaatcgtc
     8041 cacctgatcc tgactggcta ccacaccggc tttgctggta tcgacctgag gtccattaag
     8101 caaactctgg ttttcacgtt ttggacgcga ctcctgttcc gggatgtttt ccaacagcac
     8161 catcagcaac tggcgttcga tctcctgaat gacatccatc atccgcttaa tgacctgccc
     8221 ggtgagatcc tgaaaatcct gcgccatcat gatttccagc agttgcgcgt tagtaaagct
     8281 ggtatgcgcg ggtacatctg ccagaaattt ccgcgtatct gttaccagtt cacgggcgtc
     8341 ggcaaggtca atcggatcgg caaaccagtc atcccaacgt tgggttaacg cttttgctga
     8401 tttctccatt tgatcctgat gcggttgtga cgcctcaaca ctgttcagcg cccgctccgc
     8461 agcctgggcg gtcatctgca caacatagta caaacgatcg cgcgcatcgg ggatggcttc
     8521 cgccgcttcg gcaatggcct gatccagtcc cagttcccgc aaactgtcgc gcagcatacg
     8581 cgtcaggctg ccgatgcgcg caatgatatc gccagctgaa tgctcgtcag caggtttgat
     8641 tgatggttgc atcatagtcg catcctcaca tgcccagttt ctcaaagatt ttgttgagtt
     8701 tttcctccag cgtcgcggcg gtaaatggct tcaccacata gccactggcc cccgcttgcg
     8761 ccgcagcaat gatgttctct ttcttcgctt ctgcagtcac cattaacact ggcaatgccg
     8821 acatcgcgcc atccgcacga atcgttttca gcaattccag gccatccatg ttgggcatgt
     8881 tccagtcgga gataacaaat ccataaccgc ctgcctgcaa cttattgaga gcgtcgacgc
     8941 catcttccgc ttcctcaaca ttattgaatc ccagctcttt cagcaggtta cgcactatgc
     9001 gtcgcatggt ggaaaagtca tccacaacca aaaatttaag ttctttatcc gccatttcac
     9061 actcctgatt taaatacgta tcgcctgtcc ggcactaatt tttgccaaca tttgctggct
     9121 tacctggtta agatcgacca cttcgcagac accacccata ttgatggcct cgcgcggcat
     9181 gccgaacacc acgcaacttg cttcgttttg cgcaagggtc catgccccag cctgacgcat
     9241 cgccaacatt cccgccgcgc cgtcgttgcc cataccggtc aggatcaccc caaccgcatt
     9301 acgccccgcc tgtttggcga cagaatggaa caacacatct accgaaggcc gatgacggtt
     9361 aaccgccggg ccatcgtgaa ttttgatttg gtaatttgcg ccactacgcg ccagctccat
     9421 atgccgatcg cccggcgcaa tataggcatg ccccggcaaa acacgttccc cgtcttcggc
     9481 ttctttaacc ccgatctggc aaagcttatt aagtctgtcg gcaaaagagc gggtgaaacc
     9541 gggcggcata tgctgggtaa ttaacagtgc cgggctggaa agcggcaacg gttgcagtac
     9601 gtgacgaatt gcctcagttc cacccgttga agcaccaatc gcaatcagtt tttcagaact
     9661 caacaacggc cctgccttca gcgttgtcgg tgccgacaat ggcttatgtg ctgcaaggct
     9721 cgcctttgct gctgtacgca ccttttcagc aatcatttcg ctgtacgcca gcattccttc
     9781 gcgaataccc agttgcggtt tggtgacaaa atctattgcc cccagctcca gcgcgcgcag
     9841 cgtgacttct gatcctttgc cggtcaggga agaaaccatc acaacgggca ttggacgcaa
     9901 acgcattaat ttttcgagga aatccagtcc gtccatccgc ggcatttcaa catccagcgt
     9961 cagcacatcg ggattgaatt tcttaatcaa gtcacgcgcg accagcggat caggcgcggt
    10021 cgccaccatt tccatgtcgc tatggctgtt gatgatttct gtcatgatct ggcgcatcag
    10081 tgccgaatca tcgacagata acaccctgat tttgctcatc gttaatcctt acttagcgca
    10141 tacaccgttt gaccacgcag cgtgaagcgg cgctcgaggt ggctaaagtt ttcagagtga
    10201 cccgcaaaca gcaatccgtc gggtttaagg agcggaacaa agcggcgcaa aatctcctgc
    10261 tgggtagttt gatcgaagta gatcatgacg ttacgacaga agatcgcatc aaacggcccc
    10321 ggcacggtgt actgtttcgc cagtagattc agcgaggcaa aatcaacata gttcgccagc
    10381 tcctgacgca cgcgtaccag cccttcatgc ggtcccgtcc ctcgcatgaa ataccgttgc
    10441 agttgctgcg gcgtcaggtt tttcaactct tcatggcgat agataccgct tctggctttt
    10501 tccagcactt cggtgtcgat atcactggca aacactttcc agcgtccggg cgcggtgccc
    10561 aatgtgtcag ccagcgtcat cgcaatgctg tacggctctt cgccggtcga agccgccgcg
    10621 ctccatacgc gatactcgcc agaacggcga cgtgcgtgat ccgcgagcag agggaaatga
    10681 tgtgcctcac ggaaaaatgc cgtcagattc gtggtcagcg aattgataaa cgcctgccac
    10741 tcaccgctgt gctgattaga ttccagcaag ttcagataat gaccgaaatc cgtcagtccc
    10801 agcgaacgca aacgacgaac cagtcggttg taaaccatgt cgcgtttatg gtcagccaga
    10861 acgatcccgg ctcgttgata gatcaattga cttatccgcc gaaaatgcgc gtcggaaagc
    10921 gccaggcgct cggtcatctg taacaataaa gacgtttgcc cacagggcag agatgaagtc
    10981 atagcgcctt ctcaatcact tcaggatacc actggcgcaa tttgtaactg cgccgactca
    11041 tgtcgtgcta cttcatgttc ttcaagggta aataccgcca cacgcgacga aagatggtcg
    11101 gcctgattcg ccagttgttc cgttgccacc gccgcttctt ctaccagcga ggcgttctgc
    11161 tgtgtcacct gatccatctg gctgacagcc tgcgcaacct gctcaatccc ccgccgttgt
    11221 tcttccgagg ccgaagcaat ttctcccata atgtcgttca cgcgggtcac cgaactgaca
    11281 atatcggtca tggtcgcggc ggcgttattc accagtttcg aaccctgctg gacacgattg
    11341 actgactctt cgatcagccc tttgatctct tttgccgcct gggcgcttcg gctggcaaga
    11401 ttgcgcactt ctcctgccac taccgcaaaa ccacgcccct gctctccggc gcgagccgct
    11461 tctactgccg cattcagggc cagaatattg gtctggaaag caattccgtc gataacgctg
    11521 ataatgtcgc caattttttg cgagctggtg gcgatctcct gcatggtgtg agtcatggta
    11581 ctgacctgaa caccgcccgc ctgcgccgtt gtcgcggcat tttttgcaag ttccgacgcc
    11641 tgtcgtgcgt tatcggcgtt ttgccctacc gtggcggtta attgctccat actggcggcc
    11701 gtttgtgcca gcgatgccgc ctgctgttcg gtacgacttg agagatcgtt attgcctgcg
    11761 acaatctccg cgataccaat gtgcatctcc tggcttccct tacgcacatc acttaccgtc
    11821 ccacgcaaag cctgctgcat ggtcttcagg ctggcaaaaa tggcggtgat ctcattacga
    11881 ccatataccg caatcggacg cgccagatta cccgcagcaa tgctgtcaaa atggctaccg
    11941 ataatggcca gtggttgaac aatcatcttg cgcgtccacc acagcgcact gctgatgtag
    12001 atcgccgcaa caataatcat gctgataaac accagcgccg aaatctgata gttacgctgg
    12061 ctttgcgcac tggcggcttc cagcacatgg atgatctcca gctgccaggc ctcaaagtta
    12121 acgtcaaacg cattctgtga tccctgcacc ggcgcagtga ggaaatccga aagttgatta
    12181 ctttcaagcc aggtcgcctg atgttcgaga tcgttgtgcc agcgggcaaa acttttctcc
    12241 gtctcttttt gcaatgccct gacgtgctcg ttgcccgcag tcatcgccat aaaactttta
    12301 aacagcgtgg tggattgcgt cagactggca cgcgccgtcg tcatcaacgt tttaatgtca
    12361 tccgccggat agctaagcgc tgtcagagtg cctgctttgt tcagcgtggt actggcctgt
    12421 aacattaccg cccgagtttg cgctaatgcc gcacgttgct gattgctctg ctcgacctga
    12481 ttcagtcgtt gcaaatcgtc gcgaaatgcc caaaaagaca tgccgttact gccaatctgc
    12541 aagatcccgc agagaatcaa aattaaaaac agcgtggtcg aaattcgaat acgattaaac
    12601 atcaacgctc cccatcaggc ggcaatgacc gcgttagtaa atactcgtca aaatgtttcc
    12661 cagtttggat cttgttcagc aattcgcagt cgtggctgtg ccggtggttg ctcactggca
    12721 ggacgggatg gtgtttgcgg tttattggtg agtgggctgg ctgccagacg aaacgcggaa
    12781 accgcttgcg ttaaacgact agcctgttct tccagcgcag ctgcagcggc agctgattcc
    12841 tgcaccagcg atgcgttctg ttgcgtgacg tgatccattt ccgaaaccgc caatgcgact
    12901 tgatcaatgc cacggctctg ttcatccgat gccgatgcaa tctcgcccat aatgtcagtc
    12961 acgcgagtga cagcatcgac gatattgttc attgtttccc cggcgctttc gaccagcacc
    13021 gaaccggtat caacgcgcga gacggagtct tcaatgagag ctttgatctc ttttgccgcc
    13081 tgggcgctac gactggcaag attacgcact tcgcccgcca ccacggcaaa accacggccc
    13141 tgttcacccg ctcgcgcggc ttcaacagcg gcattcagcg cgaggatatt agtctggaag
    13201 gcaataccgt cgattacgct gataatgtcg gcgattttct tcgaactatc ggcgatctca
    13261 tgcatcgttt tcactacgcc atccaccact ttgccgccgt gctgggcggt gtcggaggca
    13321 ctttgcgcca gttgcgaggc ctggcgggcg ttatcggcgt tttgcttcac tgtcgcggtg
    13381 agctgctcca tgctggcggc agtttcttcc agcgcggatg cctgctgttc cgtacgggag
    13441 gaaagatcgg tgttgcccgc cgcaatttca cgggtaccgg catagatggc atctgaacct
    13501 tcgcggacat gagtgacggt gtcagtcaaa gagcgttgca tatgtgaaac gctctgcgcc
    13561 aggtcgccca tttcactgcg cccgtcaatg gtcagggtat tcgccaggtt accaccggcg
    13621 atttcgcgaa tgtgagcaat aatttttgcc agcggtgtaa gcaacatacg gcgaatgcca
    13681 taccacgcca ccagcagaat caataccacc accagcgcga taaccgccag ttgccactgg
    13741 gcaaatcggt aatcatctgc gttgtcagtg acgatatcgc gatacagttt ttcactgctg
    13801 agggcgtact gagcaaacgc ttcgcccatt gcattttgca ttccctgggt tggctgagcg
    13861 aaataagctc cagtattgcc ataatcaaga taatcaatca gttcagttaa cgctgtgtaa
    13921 tagtttttat atttttcatc aatattacga ctggtagcga ccatttcagg taacggtgcc
    13981 atgcttttga attttttata atgagtcgcg gcctgggcca atgttttcct ggcgctatcg
    14041 agcaattcaa ctttggcatt actttgttga ttggaggaat ccatcatcat tcgcaccgct
    14101 gaacgactca ggttaatgcg cgtttgcagc attaaatccc aggttgacgt cagctcgccc
    14161 tgctgttctc gtaattgatt ggaaaccaca aagctcttct ggctatggtg aagggaagaa
    14221 aaaaacagac tgccggaaat aagctgtaac agtgcgaata cccccagcac cattaccaac
    14281 agcgtgacta cgcggatacg gttaatcata aggcaccttc ctgaaaacaa gttgatctcg
    14341 ttatcggcaa ggaaggggga aactttattg ctgatgccac ccgccgcgaa attgaaataa
    14401 aacccgatgc gcagatcatc gggttcattt caattgagga aatcaggaga attacgccac
    14461 ttctgacgcc gcgctatcta acagcgccat ctcttcgctg ttcagcagtt tttcgatgtt
    14521 caccagaatc aacatacggt cgcccagtgc gcccagtcca gtgagatatt ctgttgaaag
    14581 cgtcacggca aactctggtg ccggacgaat ttgctccgcc gtcaatgaaa gcacgtctga
    14641 aacgccgtca accacgatgc cgaccacccg ctgtccgaga ttcaggacga taactaccgt
    14701 gttgtcgtta tagtccacat ccacctggct gaacttaatt cgtaagtcaa caatcggcac
    14761 aataacgccg cgcagattcg tgacgccttt gataaacgct ggcgtgttcg caatccgtgt
    14821 tacctgatcg tagccacgga tctcctgcac tttcaggata tcaataccgt actcttcatc
    14881 accaagggta aataccagaa attcctggcc tgacggctcg ctggccagct ttgttacatt
    14941 cgtcataccg gtcatattgt taccttttta ctcattcagg cggcggtgtt cgccatacgt
    15001 tgttcgcggt ttatcgcctg caaggcggag acatcaacaa tcagtgccac gctgccgtcg
    15061 ccaagaatgg tcgcagcaga aatgccgggg actttgcgat agttactttc aaggttttta
    15121 actacaacct gatgttgacc aattaattga tccaccagca aggcgtagcg gcgaccgcca
    15181 ctttgtaaga tcaccacaat tccctgggta gcttcggttt tcgcgcccgc gacgttgaac
    15241 actttccaca gttcgacgat gggcagatat tcaccccgca cttccagcac ccgctcgccg
    15301 ccggccagtg gatgcagatc ggcttcacgg ggttgcagtg attccataac agcattcagc
    15361 ggcagaatga atacttcatc cgcaacgcgt acggacatgc cgtcgaggat ggccagcgtc
    15421 agcggcagta aaatgcggat cgtagtgcca gtaccctgct tcgactggat ttcaacatga
    15481 ccgcccatct cctggatatt acgtttaacg acgtccatgc cgacgccgcg cccggagacg
    15541 tcggtgacct gctctgccgt ggagaagcct ggtgcaaata tcagcatcgc gacttcgtcg
    15601 tcgctcatgt tttcgctgac agtcaaacct tgcgaggccg cttttgccag aattcgctca
    15661 cggtttagcc ccgccccatc gtcggtcact tcaatgcaaa tgttgccgcc ctgatgttcg
    15721 gcagacagaa ttaaatttcc gacgctgttt ttacctgcgg cgagccgttt ttctggcagt
    15781 tcaataccgt gatcgaggct attgcgtacc aggtgggtca gcgggtcgat aatgcgttct
    15841 atcaggctct tgtcgagctc ggtggaactg cccaccagcg tcagttctac ctgcttgccg
    15901 agttttcccg ccagatcacg caccagccgg ggatagcgac taaagacata ttccatcggc
    15961 atcatgcgaa tcgacatcac tgattcctgc aaatcacggg cgttacgttg taactgcccc
    16021 atgctggtaa tcaaatcacc atgattaacc gggtccagtt cgctggaacg ctgggcaagc
    16081 atggactggg tgataaccag ctcgccgacg aggttaatta attgatcaac cttttctacc
    16141 gctacacgga tgctggtgga ttcactgcta cgcgtcgttt tttcccgctc cacgcgaccg
    16201 gttggcgctt gttcggctgc cagtttaagc actggtgggg tggatatttt tggcgagact
    16261 tctactgttt caaaggtaat ctgatcggct tcaatcacaa aacagagtac cgctgtgatg
    16321 tcatcttcgg cgatatcgcc cggtaatatt gccgagagag aatccgcccc tttcaccacg
    16381 tcagttaacg ttgtcagatg ccccagctct tcttccagca ggtcgacttc gctggccttc
    16441 aggcgcgaaa ggataattcg tcgcggcaac tgactgcgac tctgctcatc ttgcggttca
    16501 cttttggcaa ccacacttaa tcgggtcact gcggatggcg tttcgccttt cgcttctaat
    16561 gccagttgac gcaaggcctg gcagatataa tcgaagctgg cggcatccgg ctcttgcgac
    16621 tgtttataag cgtcgagctg ttcttgcatg atgtccttcg tttccaaaaa cagattgata
    16681 atgtcggtgt tgagttgcat ctcacctcgt ctggcttcat cgagcaggtt ttccatcaga
    16741 tgcgtggttt cctgcaaaac gctgaagcca aaagttcctg cccctccttt gatcgagtgg
    16801 gcagcccgaa agatggcatt caattgttcg gcatctggcg cttccggctg caaaaccagc
    16861 agatgctgct ccatatcagc caacagttcg tccgcttcat caaaaaatgt ctgataaaaa
    16921 tcgcttatat ccatgctcac gctgtcacct cggttcggct gatggcattg tgggaacaca
    16981 gacctgtggt gcaacctcag gtttttccag ggcgcttact ggctcattct ggctttcggc
    17041 gttttcatgc aaaatggcct gttcggcttg tttgttcagt accagcaggc tgatgcgacg
    17101 gttgacggca tcatcaggtc cgcgatcgct taagcgcatc gttgccgcca tgccgaccac
    17161 acgtaacact ttgccgctat ccaaccctcc gaccatcagt tcgcggcggg atgcattggc
    17221 ccgatcggca gaaagctccc agttgctgta acctttctca ccactggcgt aggggaaatc
    17281 atcggtatga cccgaaaggc taatacggtt gggaatacca ttcagaacgg gcgcgatggc
    17341 acgcagaatg tcgcgcatat agggttcgac gtcggcactg ccagttctaa acatcgggcg
    17401 attctggcta tcgatgatct gaatacgtag accttcctgg accagatcga ttttgagatg
    17461 tggacgtaac gcccgcagtt tcggatcgga ctctatcaat tggtcgagat cgccacgcaa
    17521 tttccgcaat cgactttgct ccatgcgttt tttcaactct tcgatgttcg gctgcttatt
    17581 cacttccccc tggctttggg tgtaatcatc gccaccgccg ggaattgggc tttcactatt
    17641 agaaatgcga tcgccgcccg taaccgcagt cgccagtgga gtccggaagt actccgcaat
    17701 ctgaatcaac tcttttgggc tggagatgga gatcagccac atcaccagaa aaaaggccat
    17761 catcgcggtc ataaagtcgg cataagcaat cttccacgat ccatgtgctg ccccgtggct
    17821 tttggctttg cgtcgtttga cgacaataat cggatgcgct tgattcttca tgcttcctcg
    17881 gttgtcgtct gttgttgcgg atttttcacc gcacgcacat gctcttccag ttcaataaac
    17941 gacggacgtt cgctggaata gagcgttttg cgaccaaact caacggcgat aggcggtgcg
    18001 taaccgttca gattagaaag cagtgtgact ttgacgcact gcatcatttt gctggtttcg
    18061 gcgcttttct gacgtaaaac actcgctaat ggggaaataa atccgtaagc caataaaatg
    18121 ccgaggaaag tccccaccat cgcatgtgcg ataagcgccc ccagctcggc ggcaggacga
    18181 tcggctgaac ctaacgcgtg aacgaccccc attacagccg cgacaatacc aaacgccgga
    18241 agtgagtccc cgaccagcgc cagactgttc gccgggactt ctgcctcgct ttcgtgcgtc
    18301 tcaatctctt catccatcag ggcttcgatt tcgaaggtgt tcatgtgacc gctgataatc
    18361 aggcgcagat aatcgacgat aaaatcaagc atgacgctat ccgcgatgat gcgtgggtag
    18421 ctggcgaaga tctcgctctc acggggattt tcaatatcac gttccagcga aaacatcccc
    18481 atctgccgcg atttcgccat caaccgataa agcagagcca gcagatccat atacattgct
    18541 ttggtgtatt tggagcgacg aaacagcaac ggcagcgctt tcagcgtgcc tttaatcgct
    18601 tttccattat tgccgacaat aaacgaccca atccctgcac cggcaataat caccagttca
    18661 gcgggttgat agagtgctcc aaggcttcca ccggtcatca aataaccgcc gaaaactgta
    18721 ccgagaacaa ccaggtaacc taataagata agcacgacat catccttcca ctgttgacca
    18781 tgacaggatg ttcagtcgtc aggcgttaac gcgcgattgg ggcaaaaaaa agcagcggta
    18841 cgtcgttacc gctgctggaa tgttgcgcca caccgtatca gttaaacagc ctgtactctc
    18901 tgttcatcca gcagttgtgg gataatatcg gcaggattct gggaaagttt acgtcttttt
    18961 actgcccggg atggcggttg acataagctg caggcaaagc tgccaacagg ctggtgagcg
    19021 tgggtaataa aattgccgcc gcagcagttg cagctggaaa gttgcagtaa tccactttca
    19081 acaaaccgca ccaatgtcca ggcacgggtt aatgccagca gcggtccttc ttctgcttgc
    19141 gggcactgtt caaggtataa acggtaggct ttgatcaccg catcgacgcc attacacaaa
    19201 ccggttttca gtaaaaactg ccatgcatta cagaacatcg aagcatgaac gttttgttcc
    19261 caagtcataa accagtcggt tgagaatggc agcatgcctt tcggcggtgg gcttccgcgc
    19321 agttctttat aaagttttat caggcgtccg cgacttaact gtgtttcgct ttccagcatc
    19381 tgcaaacgag cgcccagggt gatcaattcc attgccagct gaatatcccg cgcttcctga
    19441 acaatgcttt tttcactcat gatcaggccc ttttcttgcg cagcgcttct tcaggctgat
    19501 taacatcatt cagcaagcgt gttgagagca tgatgccggt atgaatttgc tggagatcgt
    19561 caacgcggga atcttgcgtc aacagagtaa tcgtctggtg gctgtcaaaa cggaagtgac
    19621 aaaccagctg attggtttct gccagcttaa ccatttgcgg aagagtcagt gccgctaatg
    19681 ttgtcgccat ttcttcattt atgccgagac gaaacatagc ggacgctttg tcctgaacaa
    19741 tcagacgctg tgcaagtagt aaatatgaca agttgatgtc ataaatgtgt ttcagcaact
    19801 cggaggtatg cattattccc acccagaata accaacttta tttttatgcg gtttcaccgc
    19861 accccgtgat gtcgccggga agccccggta aaaaataatt agcattagaa tagttgcgat
    19921 aagctgcaat aagcagagcc acctttttgg tttaataagt ccttacaaat agaagggggt
    19981 ctttacactt atctaagatt tttcctaaat cgacgcaact gtactcgtca ctacacgcac
    20041 atacaacgga ggggggctgc gattttcaat aatgtgtgat gcagatcaca caaaacactc
    20101 aattacttaa cataaatgta taagtcatac ttttgttttg ggtgtatttc aatctgttaa
    20161 aaagtttttc gctacgctag caagcaaaaa tgaaacagga ataatcgaaa tgggatgttg
    20221 cgcacagtta aaataactca ccgtaaataa tcatctgcta taaataatca ctttcatgca
    20281 ataccagata agctattttt aaacagacac ttaccgcaca acaaactaat taaatacaaa
    20341 gaa
//
LOCUS       DAZFAB010000082        19884 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZFAB010000082 DAZFAB010000000
VERSION     DAZFAB010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19884
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.082"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..88)
                     /locus_tag="ACHJWA_003386"
     CDS             complement(<1..88)
                     /locus_tag="ACHJWA_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4309918.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKSSVD"
     gene            106..297
                     /gene="yfgG"
                     /locus_tag="ACHJWA_003387"
     CDS             106..297
                     /gene="yfgG"
                     /locus_tag="ACHJWA_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFO4309919.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            608..1126
                     /gene="yfgH"
                     /locus_tag="ACHJWA_003388"
     CDS             608..1126
                     /gene="yfgH"
                     /locus_tag="ACHJWA_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO4309920.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            1142..1681
                     /gene="yfgI"
                     /locus_tag="ACHJWA_003389"
     CDS             1142..1681
                     /gene="yfgI"
                     /locus_tag="ACHJWA_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFO4309921.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1774..3351)
                     /gene="guaA"
                     /locus_tag="ACHJWA_003390"
     CDS             complement(1774..3351)
                     /gene="guaA"
                     /locus_tag="ACHJWA_003390"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFO4309922.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(3420..4886)
                     /gene="guaB"
                     /locus_tag="ACHJWA_003391"
     CDS             complement(3420..4886)
                     /gene="guaB"
                     /locus_tag="ACHJWA_003391"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFO4309923.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            5048..6418
                     /gene="xseA"
                     /locus_tag="ACHJWA_003392"
     CDS             5048..6418
                     /gene="xseA"
                     /locus_tag="ACHJWA_003392"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFO4309924.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDLQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQCNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGKVLKKVKQVKAGEMLTTRLEDGWVESEVKNIQPVKK
                     SRKKVH"
     gene            complement(6415..6630)
                     /gene="yfgJ"
                     /locus_tag="ACHJWA_003393"
     CDS             complement(6415..6630)
                     /gene="yfgJ"
                     /locus_tag="ACHJWA_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO4309925.1"
                     /translation="MELHCPQCQHVLDQDNGHAYCPSCGKVIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6699..8171)
                     /gene="der"
                     /locus_tag="ACHJWA_003394"
     CDS             complement(6699..8171)
                     /gene="der"
                     /locus_tag="ACHJWA_003394"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFO4309926.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(8289..9467)
                     /gene="bamB"
                     /locus_tag="ACHJWA_003395"
     CDS             complement(8289..9467)
                     /gene="bamB"
                     /locus_tag="ACHJWA_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFO4309927.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPAVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(9478..10098)
                     /gene="yfgM"
                     /locus_tag="ACHJWA_003396"
     CDS             complement(9478..10098)
                     /gene="yfgM"
                     /locus_tag="ACHJWA_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFO4309928.1"
                     /translation="MEIYENENDQVEAIKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(10116..11390)
                     /gene="hisS"
                     /locus_tag="ACHJWA_003397"
     CDS             complement(10116..11390)
                     /gene="hisS"
                     /locus_tag="ACHJWA_003397"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFO4309929.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(11501..12619)
                     /gene="ispG"
                     /locus_tag="ACHJWA_003398"
                     /gene_synonym="gcpE"
     CDS             complement(11501..12619)
                     /gene="ispG"
                     /locus_tag="ACHJWA_003398"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFO4309930.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12646..13659)
                     /gene="rodZ"
                     /locus_tag="ACHJWA_003399"
     CDS             complement(12646..13659)
                     /gene="rodZ"
                     /locus_tag="ACHJWA_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFO4309931.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPVVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTAATTPDGAAPLPTDQAGVTTPVADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13944..15098)
                     /gene="rlmN"
                     /locus_tag="ACHJWA_003400"
     CDS             complement(13944..15098)
                     /gene="rlmN"
                     /locus_tag="ACHJWA_003400"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFO4309932.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPAAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(15248..15679)
                     /gene="ndk"
                     /locus_tag="ACHJWA_003401"
     CDS             complement(15248..15679)
                     /gene="ndk"
                     /locus_tag="ACHJWA_003401"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFO4309933.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15820..16674)
                     /locus_tag="ACHJWA_003402"
     CDS             complement(15820..16674)
                     /locus_tag="ACHJWA_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO4309934.1"
                     /translation="MLSHNITIGAECARQRFRFATCHACVEACPAQALTLTEEGIAVD
                     AEQCIDCAVCQFICPQEAIRGVNSPLRYFCNHTLVAPFTTIAPTLEELLLWHKEQGIT
                     AIAIDLSQAKAWERALLLLNECLRQYGEEGWSVVSPHTSEVNVSRRRLFHVQRADVQM
                     TQIQPGLRRLHYLWPQISDARPMMDPQKCQLCGACWRACEQQVFSLNEGHLQINDALC
                     NGCQNCIAVCFHQAMTVELTILPAKIVNLHANRKVCKTCQKSFLTFQQNAQNCLYCQR
                     HRYGMRTP"
     gene            complement(16674..17495)
                     /locus_tag="ACHJWA_003403"
     CDS             complement(16674..17495)
                     /locus_tag="ACHJWA_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     family protein"
                     /protein_id="HFO4309935.1"
                     /translation="MHELPLMAFTLLLQASVGMTLWLAFLHRHILITSSARPVMRPPL
                     LLAFLAGAVGLLISTLHLGYPLNAMHALSHFSSSWLSREIIFGALYLALLGLSTLLVM
                     LRKGGWQLLLMLAAVVGIVDVFCMAQVYIHTSIITWQHYNTLIMFLGTVGILGSALVA
                     VFRISGILPQIDALRNGCVLVIALLVLLRLLVQPLWIGDLTANAMQIATLPHAPLAML
                     GQLKPILTLSWGISVIGMMFFAVGGCKKNIPAALFGSVMLVGSEVMLRFVFFSIG"
     gene            complement(17488..18117)
                     /locus_tag="ACHJWA_003404"
     CDS             complement(17488..18117)
                     /locus_tag="ACHJWA_003404"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex B
                     subunit"
                     /protein_id="HFO4309936.1"
                     /translation="MSQFKEFASVSDKQLGFFIDSSRCSGCKACQVACKDKNNLEVGR
                     RFRRVYEVNGGGFIATGEGGVSHNVFAYTLSVSCNHCADPICTKNCPTMAMHKRPGDG
                     IVRVNTDKCVGCGYCAWSCPYGAPQMNEQTGQMSKCDFCIDLQAKGEQPICVATCPLG
                     AIKFGPIDELREKYGDVSDVKGLPDSSITQPNLVIKPHQGAEKEASHHA"
     gene            complement(18114..>19884)
                     /locus_tag="ACHJWA_003405"
     CDS             complement(18114..>19884)
                     /locus_tag="ACHJWA_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex A
                     subunit"
                     /protein_id="HFO4309937.1"
                     /translation="TPYTYGVAASGNSMDTLLDTKLVILWGHNPTETIFGHTNYYFQK
                     MKQNGTRFIVVDPRYSDTVSSLADQWIPLLPTTDNALMDAMMYVIISENLHDKTFIDT
                     YTLGFDENSMPEGVPANESLVAYLFGAKDGIHKTPEWAEKITHVPAQSIRQLARDYAT
                     TKPAALIQGWGPQRHICGERTARGSTLLASITGNVGIKGGWAAGYGGSSNRKFCVGPD
                     MPENPVQAKISIMNWMQAADDASKVTPQDGLKGVDKLDSNIRLLFSLAGNYLANQNPD
                     VHQAAKLLEDESKIEFIVLSDLFMTPSAKYADVLLPETSFMERWNIGETWGTASYLIL
                     SEKLIEPDFERRTDYDWLRDVAKKLGVEAEFSQGRDEKQWIEHIWEQTRLAMPDENLP
                     DFATLQKTRRHLFKSAPHIAFEANIRDPQNNPFPTPSGKIEIFSKRLFDMQDPEIPAL
                     SHYVPAFEGPEDKLTAKYPLQLITWKGKNRANSTQYANPWLQEVQTQKLWLNPQDAKQ
                     RGISEGDSVKIYNDRGVSIIPVEITPRIIPGVVAMQAGAWWQPDAQGIDRGGCANVLS
                     STRITALAKGNSHQTMLVEVEKA"
BASE COUNT         4917 a         5380 c         4802 g         4785 t
ORIGIN      
        1 tatccactga cgatttttta cataacgaag aattgaccat tttgtcctgt tgtgccttaa
       61 tgtaagtacc gtccacggcg tgggacatac ttcaaggaac cttttgtgag tcaggcaacc
      121 agtatgcgaa aacgacaccg atttaacagt cgcatgaccc gtatcgtact gctcatcagc
      181 tttatcttct tctttggccg ttttatctac tcgtccgtcg gtgcctggca gcaccatcag
      241 agcaaaaaag aagctcagca atccacactc tccgtcgaat caccggtaca acgttagcgg
      301 ttaccttctc cactttcaca gaacataacg gcgcttcgct gtcggatgct tttgctcttt
      361 ggaattatca aagcggcaga tattctttca tcttaaatta tacgtctttg tcccgactga
      421 tgtttatcct gtttggctgc gaaataaata taaaattaat atatatgttg taatgatgta
      481 tttttataaa ttattcccgg cgtgaatttt aataaattta atccatccct ttatacgcaa
      541 tacatttact ttcctctttt gatgatctta aatgtcttat ttttcgtaat gtgtataaca
      601 aggaatagtg atgaaattta aaaaatgtct tctgcctgtg gcaatgttag cgtcattcac
      661 tctggcagga tgccagtcaa atgctgacga tcatgccgcc gatgtttatc aaaccgatca
      721 actgaatacc aaacaagaaa ctaaaaccgt taatattatt tccattcttc ccgcaaaagt
      781 tgccgtagac aactcccaaa ataaacggaa cgcacaagcc ttcggcgcgc ttattggcgc
      841 agtcgctggc ggtgttatcg gccacaacgt cgggtctggc agcaattccg gaacgacggc
      901 aggtgcagtt ggcggcggag ctgtaggcgc ggcagcgggt tctatggtga atgataaaac
      961 cttagtggaa ggtgtttctt taacgtataa ggaaggcacc aaagtgtata cctctactca
     1021 ggtgggtaaa gagtgccagt ttacgacagg tttagccgtt gttattacca cgacgtataa
     1081 cgaaacgcgt attcagccaa ataccaaatg tcctgaaaag agctaataat caggaggagt
     1141 catgaagaaa gtttttcttt gcgccatctt agcctcctta agctatccgg ctatcgcctc
     1201 atcattgcag gatcaactct ctgctgtcgc agaagcggaa cagcaaggta aaaatgaaga
     1261 gcaaaggcag catgacgaat gggtcgcgga gcgcaacagg gaaatccagc aagagaagca
     1321 acgtcgcgca aatgcccagg ctgccgctaa caaaagagcg gcaacggcag cggcaaataa
     1381 gaaagctcgt caggataaac tggacgccga agccactgcg gacaaaaaac gcgatcaaag
     1441 ttatgaagat gagctacgca gcttagagat tcagaaacaa aaactggcgc tggcaaaaga
     1501 agaagcccgc gttaagcgag aaaacgaatt tatcgatcag gagctgaagc acaaagccgc
     1561 gcaaactgat gtcgtgcaat ctgaagctga cgccaacaga aatatgactg aaggcggtcg
     1621 cgatctgatg aaaagcgtgg gcaaagcaga agagaataaa tcggacagct ggtttaatta
     1681 atcgatgtta gtaacttcaa gcctataatt cttgaagata aaaaaccctc tgtagtaaca
     1741 gagggttttg ttcattcata gtgcagggtc aaatcattcc cactcaatgg tagctggtgg
     1801 cttgccgctg atgtcataca ccacgcggga aataccgttc acttcattga taatgcggtt
     1861 ggaaacgcga ccgaggaaat cgtacggcag atgcgcccag tgtgcggtca taaagtcgat
     1921 ggtttcgaca gcacgcagag agacaaccca gtcatactta cgaccatcgc ccattacgcc
     1981 aacggaacgt actggcagga agacggtgaa cgcctggctg actttgtcgt acaggtccgc
     2041 tttacgcagt tcttcaatga agatggcgtc agcacggcgc agcaggtcac agtactcttt
     2101 cttcacttca cccagaacac gaacgccaag gcctggtccc gggaacgggt gacggtacag
     2161 catgtcgtac ggcaggccca gctccagacc aatcttacgc acttcgtctt tgaacagctc
     2221 tttcagcggt tcaaccaggc ccatcttcat ctctttcggc aggccgccca cgttgtggtg
     2281 agatttgatg acgtgtgctt taccggttgc agacgccgca gattcgataa cgtcagggta
     2341 gatggtgccc tgcgccagcc acttcacgtc ttccagtttc agcgcttctt catcgaatac
     2401 ttcaacgaaa acgcgaccga tgattttacg ttttgcttcc ggatcgtttt cgccagccag
     2461 cgctgacagg aagcgatctt ctgccggtac gtgaacaatg ttaagaccaa agtgatcgcc
     2521 aaacatatcc agaacctgct ctgcttcgtt gagacgcagc aggccgttgt cgacgaatac
     2581 gcaagtcagg tttttaccga tagcgcggtg cagcagcatt gcggttacgg aggaatccac
     2641 accaccagag aggccgagga tgactttatc gtcgcctacc tgctcgcgga tgcgagctac
     2701 agcatcgtcg ataattttcg ctggcgtcca cagggcttca cactggcaga tatcacgcac
     2761 aaaacgctcc agcatgcgca taccctggcg ggtgtgagtc acttccgggt ggaactgtac
     2821 gccatagaag cgtttttctt cgttagccat aatggcaaac gggcagcttt cggtgctggc
     2881 tacggtgacg aagtcggacg ggatagcggt aactttatcg ccgtggctca tccagacatc
     2941 gagcagcggt ttaccgtctg cggtcagcgc atcttcgata ccgcgaacca gtgcgctgtc
     3001 gtttacgact tcaacctgcg cgtagccaaa ttcacgttcg ttagaggctt caacgtgacc
     3061 gcccaactgc attgccatgg tctgcatgcc atagcaaacg ccgaataccg gtacgcctgc
     3121 ttcaaagaca tactgcggcg cacgcgggct gttttcttca gtagtacttt ccgggccgcc
     3181 ggaaagaata atgccgcttg gattgaagtc acgaatttgt gcttctgtca catcccatgc
     3241 ccacagttcg cagtaaacac ccagctcacg cacgcggcgc gcaaccagtt gagtgtactg
     3301 agaaccgaag tccagaatga ggatgcgatg cttatgaatg ttttccgtca ttgacgctta
     3361 ttccgaggca agtgaaacag ataatataaa tcgcccgaca tgaagtcggg cgaagagaat
     3421 caggagccca gacggtagtt cggggactct ttagtaatgg tcacgtcgtg aacgtggctt
     3481 tcctgaatgc ccgcaccgct gatacgtaca aactccgctt tagtacgcag ttcgtcgata
     3541 gtaccacagc cggtcaaacc catacaggag cgcaggccgc ccatctgctg gtgaatgatc
     3601 tctttcaggc gacctttata agctacgcga ccttcgatac cttccggcac cagtttgtcg
     3661 gcagcgttat cgctctggaa ataacggtca gaggaacctt tggacatcgc gcccagggaa
     3721 cccataccac ggtaagattt gtaagaacgg ccctggtaga gttcgatttc acccggagat
     3781 tcttcagtac ccgccagcat ggaacctacc atcaccgcgc ttgcgccagc ggcgatagct
     3841 ttggcgatgt cgccggagaa gcgaatacca ccatctgcga taaccggaat accggtgcct
     3901 tccagggctt caactgcgtc agcaacggca gtgatctgcg gaacacctac gccagtaacg
     3961 atacgagtcg tacagataga accagggccg ataccgactt taaccgcgct gcaaccagct
     4021 tctgccagag cgcgtgcacc tgcagctgtt gccacgttgc cgccgatgat ttgcagatcc
     4081 ggatatttag cacgggtttc acggatacgc tgcaaaacgc cttctgagtg accgtgggag
     4141 gagtcgatca gcagaacgtc aacgccagcg gcaaccagcg cgtcaacacg ctcttcgtta
     4201 cccgcacctg cgccaactgc agcaccaaca cgcagacggc cttgctcgtc tttacaggcg
     4261 ttcggtttac gttccgcttt ctggaaatct ttcacggtga tcatgccgat caggtggaat
     4321 tcgtcatcaa ccaccagcgc tttttcaacg cgtttttcgt gcatttttgc cagcaccact
     4381 tcacgggctt caccttcacg cacggtgacc agacgctctt tcggcgtcat gtaaacgcta
     4441 accggctggt tcaggtcggt aacaaaacgc acgtcacgac cggtgataat ccccaccagt
     4501 tcgttttctt cggtaacgac cggatagccc gcaaaaccgt tacgctcggt cagttctttc
     4561 acttcgcgca gcgtcgtggt tggcagaaca gtctgcggat cagtcaccac accagattcg
     4621 tgttttttca cacggcgaac ttcttctgcc tggcgttcaa tggacatgtt tttgtggata
     4681 aagccgatac cgccttcctg agccagagca atagccaggc gcgcttccgt tacggtatcc
     4741 attgctgcgg aaagcatagg gatattcaga cgaatagttt tcgtcagctg ggtgctgagg
     4801 tcagcagtat tcggcagaac ggtagaatga gcaggaacga ggagaacgtc gtcaaacgtc
     4861 agagcttctt tagcgatacg tagcatgggc aatatctcga ccagagtggt taataaatat
     4921 tgccgcggca ttatacagag cgtaaccgat tgcatctacc cctttttgca aaaaatgctt
     4981 gctatccccg aagggcgggt tactatcgac tgaataacct gctgatttag aatttgatct
     5041 cgctcacatg ttaccttctc aatcccctgc aatttttacc gttagtcgcc tgaatcaaac
     5101 ggttcgtctg ctgcttgagc atgagatggg acaggtttgg atcagcggcg aaatctctaa
     5161 tttcacacaa ccggcttccg gtcactggta ctttacactc aaagacgaca ccgcccaggt
     5221 acgctgcgcg atgttccgca acagcaaccg ccgggtgacc ttccgcccac agcatgggca
     5281 acaagtttta gttcgcgcca atattacgct ctacgagccg cgcggcgact accagataat
     5341 cgttgagagt atgcagccgg ccggtgaagg gctgctgcaa cagaagtacg aacagctcaa
     5401 agcgaagttg caggctgaag gattgttcga tctgcaatac aaaaaaccac ttccctcccc
     5461 tgcgcattgc gttggtgtga tcacctcaaa aaccggtgct gcgctacatg atattttgca
     5521 tgtgttaaaa cgtcgcgatc cttctctgcc ggtgatcatc taccccaccg ccgttcaggg
     5581 cgatgacgcg ccggggcaaa ttgttcgcgc cattgaactg gcgaatcagt gcaatgagtg
     5641 cgacgtgttg atcgttgggc gcggcggcgg ttcgctggaa gatttatgga gttttaacga
     5701 cgaacgcgta gcgcgggcga tttttgccag ccgcattccg gtcgtcagcg ccgtcgggca
     5761 tgagacggat gtgaccattg ccgattttgt tgccgatctg cgtgcaccaa caccgtcggc
     5821 tgccgccgaa gtggtgagcc gtaatcagca agagttactg cgccaggtgc aatcgacccg
     5881 tcaacggctg gagatggcga tggattatta tctcgccaac cgcacacgtc gctttacgca
     5941 aattcatcac cgattacagc aacagcatcc gcagctccgg ctggcacgcc agcaaaccat
     6001 gcttgaacgc ctgcaaaaac ggatgagctt tgcgctggaa aatcaactta agcgtgccgg
     6061 gcaacagcag cagcgattaa cacagcggct gaatcagcaa aatccacagc cgaagattca
     6121 tcgcgcgcaa acgcgcattc agcaactgga atatcgttta gcagaaaccc tgcgcgcgca
     6181 gcttagcgcc acgcgtgaac gtttcggtaa tgcagtaacg cacctcgaag ccgtaagccc
     6241 actgtcaacg ctggcgcgtg gatacagcgt tactactgct actgacggca aggtactgaa
     6301 aaaagtgaag caggttaaag cgggtgaaat gctaaccaca cgtctggaag acggctgggt
     6361 agaaagtgaa gtaaaaaaca tccagccggt aaaaaaatcg cgtaaaaaag tgcattaagc
     6421 cagcacgaat tcaacgcgtt ttttcgatat caagccatgc ccgtgctgac agaagtaatc
     6481 taccgcaccg caggctttta acacctgcaa cggttgatgg cagtccgggc aaagagcttt
     6541 catttcaata actttcccgc aggatgggca atacgcatgg ccgttatcct gatcaagcac
     6601 atgctgacat tgtggacaat gcagttccat atgaattcct ccggtagcca cccctctaca
     6661 ttcatagagg gaatggcaga taaaatactt acggataatt atttattttt cttgatgtgc
     6721 ttcatcagac gcttacgttt acgcatctgg gttggcgtca gggtgttacg cttgttcgca
     6781 tacgggtttt ccccttcttt gaactgaata cgaatcggcg atcccattac gtccagcgat
     6841 ttgcggaagt agttcatcaa gtaacgcttg taggaatcag gcaggtcttt cacctgatta
     6901 ccgtgaatca ccacaatcgg cggattataa ccaccggcgt gtgcatattt cagcttcaca
     6961 cgacgaccgc gtaccagcgg cggttggtga tcttcaacag ccatcgtcat gatgcgcgtc
     7021 agcatagagg tccccacacg acgggtggag ctgtcatacg cttcacgtac tgattcaaac
     7081 aagttaccaa caccactgcc gtgcaaggca gagataaagt gcacacgagc aaaatcgata
     7141 aagcccagac ggaagtccag cgtttctttc acctgctctt tcacttcctg actcaggcca
     7201 tcccacttat tcaccacaat gacaagtgag cgcccactat tgagaataaa gcccagcagc
     7261 gagagatcct gatcggaaat accttcgcgc gcatcaatca ctaacatcac cacgttggcg
     7321 tcttcaatgg cctgcaacgt tttgattacg gagaatttct ctacagcatc ggtgattttg
     7381 ccgcgtttac gtacgccagc ggtgtcaatg agcacatact cacgtccatc gcgttccatt
     7441 ggaatgtaga tactgtcacg cgtcgtacca ggcatgtcat aaacaacaac gcgctcttca
     7501 ccaagaatac ggttagtgag tgtagactta cctacgttcg gacgacccac aatcgccagt
     7561 ttgatcggca gactttgcgg gtcgaagtcg tcttcctctt cttcttcgcc gttctcttcc
     7621 gcttcaaatt gcgcccagta ttcagcgtct tcgtcgactt cctcttgcgg tgcgagatct
     7681 tccatccacg gcagtagcac atgctccagc agacttaata cgccacgacc gtgagacgcg
     7741 gcgatcgggt agatttcacc taaaccaagc gagtagaaat caaccactgc ctgatcggga
     7801 tccagaccgt cagttttgtt tgccaccagg aaggtcggtt tttcacggga gcgcagatgt
     7861 ttggcaatcg cttcatctgc cggcatcagg cccgcgcgcg catccaccat aaacagtacg
     7921 acgtccgctt cttcaatcgc cagcagcgac tgttccgcca tgcgggtttc tacaccgtct
     7981 tctgtgccat caatcccgcc ggtatcaata cagataaact cacggccttc aatttccgca
     8041 cgaccgtact tacggtcacg agtcagaccc gggaaatccg caaccagcgc atctcgggtg
     8101 cgagttagac ggttaaataa cgtggatttt cctacgttag ggcgcccgac aagcgcgacc
     8161 acaggtacca tgtttaaagc ctcattttta taaatcatca gacaacgcac gctatattcg
     8221 cgtcgttgtt aaaaacagga aaacggcccc tgtccaggag ccgttttcaa agtgaacgac
     8281 agagacgatt aacgtgtaat agagtacacg gttccgtctt ttgcctggat cagcagtttg
     8341 ccgtcagcgg caaccggttc agtctggaaa ccggaactat caactttttg ctgggcaacg
     8401 aaacgaccat cttcgacgtt aatccagtgc aaataacctt cactgtcacc gaccaccagg
     8461 ttgccattat acagcaccgg agaagtcagc aggcgatgca gcagatcgct ttgtgtccac
     8521 agcgtaacgc cgccatcaat ggtcaacgcc atcacccggt cattttgatc gaccagatag
     8581 atgcgattgc cgtcgacgat gaaatcattc accgaaccca gttcgcgttt ccacataatc
     8641 tgaccactgc gcagatcaag cgccgtcagg ttaccattat aggccagcgc gaaaacaacg
     8701 ccgttaacaa cggcgggagt cgtgtcaaca tcgctcagac ggtcaatttc ggtagaaccg
     8761 gtcgcttggg aaatacgctg ctgccaaatc atctggccct gttccatcag cactgcgctg
     8821 acgcgaccat tatcgccccc cacgacggcc gcaccaaaag ccgttgccgg cgcagactcg
     8881 ccacgcaaag agagcgaagg catatcgagg ttaactgtcc atttgacagc gccgtcagct
     8941 tcgttcagcg cttgtaactg accgttactg gtgtggatta acaccagacc gtcgctgacc
     9001 accgggcgcg aaagtgcttc acccgcgact ttagtttgcc atgccacagt accatcgctg
     9061 gtattcagcg cgtaaacctg cgccttttcg ctgccaatgt agacatgtcc accagacacg
     9121 gtcacaccgc cagaaagtaa tgcaggctct ttagagaacc agccatcttt ctcggccagg
     9181 ctgacagacc agatttcttt gccatcatcc gcattcagcg cttttactaa accagcgcgg
     9241 tccgctgcat agacaacgtt gtccgccagt gccggatgaa gattggaata gaagttgcca
     9301 atgccgctac caacggaagt gctccacgcc gtggtcggcg taaactggtt ttcaacggtt
     9361 ggcaatgggg acatctttac cacatcttct tcgctgttaa acagcgaaca gccgcttaaa
     9421 agggtaacgg aaagcagtcc tggcagcagt aatttacgca attgcatcgg gtccctctca
     9481 gatggacaaa ttattaattt tcatctgcat catttcgctc agtgccggag taacatcgct
     9541 tttcacgcct gcttcccatg cactacgcgc accttgctta tcacctttgc tcagcaatgc
     9601 ttcaccacgc aggtcggcaa caatggcagc ccacccttca cctttgatgg tatcaagggt
     9661 tttcagcgcg gcatcagcct gcttgagctg tacctgaacg cgagcaagac gcagatttat
     9721 cacggctttg agattttcat cgctcgtgtc tgccagcccc tgttgtaact gggcggcagc
     9781 tttctctagt tcatttttgt caacaaattg ctgcgcaagt tccaaagaag ccagcgcacc
     9841 ataagtattt ttattttcag cagcaaattt ttccgccgcc gggatgctat ccggtttgcc
     9901 ttcgctcact gcggtaaccg cattttgata ggcaagagaa gcggagcgtg cagaatcaac
     9961 ctgatggctg ttccagtagc gccagccaat cagtgcgcca acgcccaaaa tcaccccaac
    10021 agccagtgct ttgccatttt cagcaaaaaa gcgtttaatc gcttctacct ggtcgttttc
    10081 gttctcgtaa atttccacgc tgtccttctc cttccttaac ccagtaacgt gcgcaaatgc
    10141 gcggctacgc tatcctgcgc aactgccgtt tgctcaccag agcgcaaatc cttcactact
    10201 gctgtgccgt tagccacttc agactcaccc agcaccacag caacgcgggc accccattta
    10261 tcagcacggg caaactgttt cttaaagttg ccgccgccgt ggttggtcat caatttcacg
    10321 cccggtaatt catcacgcag acgctcagct aatgccatag ccgcagattg tgtatcagca
    10381 cctgaagcca ccaggtatat atcgacaaca ggatcggctt taaattccgg attaacggcc
    10441 tgtactaaca atacaagacg ttcgaggccc atcgcaaaac cgacagccgg tgttgcacgg
    10501 ccgcccagtt gttccacaag accgtcataa cgaccgcctg cacacacggt gccctgggag
    10561 ccgagactgt tagtcaccca ctcgaaaacg gtacggttgt agtaatccag accacgcacc
    10621 agacgctggt ttacggtgta agcgatcccc gcgctctcaa gcagtttgca cagaccggca
    10681 aaatgctcac gagattcctc gtccagataa tcacctaatg ccggagcgtc gttgagaagc
    10741 gcctgcactt ccggattttt tgaatccagc acgcgcagcg ggttagtgta catgcggcgt
    10801 ttgcagtctt cgtccagctt ttctttatgc tgctcaagga atgccaccag cgcatcgcgg
    10861 taattggcgc gtgcttccag cgaaccgata gaattcagct caagagttac gtgttcggaa
    10921 atacccagcg cgcgccacca gcgggcggtg agcataatca gttcagcgtc gatatccgga
    10981 ccttgcagac cgaaaacttc gcagcccaac tgatggaact gacgataacg ccctttctgc
    11041 ggacgctcgt gacggaacat cggcccgata taccacagac gctgttcctg attgtacaga
    11101 agaccatgct cgatgccggc gcgtacacag cccgccgtcc cttcagggcg cagagtcagg
    11161 ctgtcgccat tgcgatcctc aaaggtgtac atctcttttt caaccacgtc ggtgacttca
    11221 ccaatcgcac gtttgaatag cggggtctgc tctacaatcg gcaagcggat ttcactgtaa
    11281 ccgtagctgc cgagcacgtt tttcagtgtg ccttcaatgc gctgccagat ggccgtttcg
    11341 ccaggcaggt aatcgttcat gccgcgaatg gcttgaatgt tttttgccac gtttattctc
    11401 tttctgaata taaaaatgaa ccctcaacgc ttccctcaat gtttcgggag ccatgcgggt
    11461 tcaatcatac acgggaagcg aggcgcttcc catcacgtta ttatttttca acctgctgaa
    11521 cgtcaattcg acgcgcttcg tccagctgac tggctttcgc acgaatgcgt gcctccagct
    11581 ggtcgatcat atcgttgttg tccagacggt ctttgcgcac gccatcttca tagagaccgc
    11641 ttttcttgtt gccgccggtg acgccgagtg tagaaaccag cgcctcaccc gggccattca
    11701 ccacgcagcc gataatcgaa acgtccatcg gagtgatgat atcttccagg cgttgctcca
    11761 gcgcgttaac tgtaccgata acatcaaatt cctgacgcga acaggtcgga caggcgatga
    11821 agttgatacc tcgcgaacgg atacgaagcg atttcaaaat atcgaaaccg actttgatct
    11881 cttcgaccgg atcggccgcc agtgatacgc gcagcgtgtc gccgatgcct tcagacagca
    11941 gcagacctaa accaatggcg gattttactg ccccgctgcg cgcgccaccc gcttcggtga
    12001 tccccagatg cagcggctga tcgatttgtt ttgccagcaa acgataagac tcaacagcga
    12061 ggaagacgtc agacgctttc acgctgactt tgaactgatc gaagttcagg cgatcgagat
    12121 gatcaacatg gcgcatggcg gattccagca acgcctgcgg cgtcggttcg ccatactttt
    12181 cttgcagatc tttttccagc gatccggcgt taacgccaat acggatcgga atgtttttat
    12241 cgcgcgcaca gtcaaccacc atgcgaatac gctcttcatt accgatattg ccagggttaa
    12301 tacgcagaca atcgacgccg tattccgcta ctttcagcgc aatgcgatag tcgaagtgga
    12361 tgtcagccac cagcggcacg ttaacctgct gtttgatgag tttgaacgct tctgccgcgt
    12421 ccatcgtcgg tacggagaca cggacgatat cagcgccaac gcgttccagc gccttgattt
    12481 gattgaccgt tgcttcgaca tctgtcgtac gcgtattggt catggactgt acggcgatgg
    12541 gagcaccatc gccaatcggc acattcccaa cgtaaatacg tgttgatttt ctacgttgaa
    12601 ttggagcctg gttatgcatg aaaaatctcc cgcgttaccc gtctgttact gcgccggtga
    12661 ttgttcggca ttgagggtca gacgcgcaac ctggttagtt ctgataaaac gactcagatc
    12721 gacaggtttc ccttgatact ggatctgtac tgcggctggc gcaccaattt tcagtttgta
    12781 cggtgcctgg ccggttaagt tcaaattacc gtctttacgc tgcataccgc taaacaattt
    12841 tttaccggta gcatcagtga cctccagcca gcaatcggca gtaaagttca tcaccagcgc
    12901 attcggatca gccaccggcg tggtcacgcc agcctgatcg gttggcaacg gcgcagcacc
    12961 atctggcgtt gttgctgccg ttggtgccgg ggtcgcggcg gtatcaacat ttgcctgcga
    13021 aggcgaaaca accgcattct gttgcggatc aacagctggt gctggcgcag ttacgacagg
    13081 tgtttgcgtg ttggttgcgg tagtatccac agacgctggc ggcgtggacg ttgtagccgg
    13141 gtctgtagtt gtcgacgtat ttaacggaac actctgcccc tgctcgctat tactgctcag
    13201 ttccgccgaa gattgatcgg ccatagtggt gatctcttcc tgctgagctt tgtggtcttg
    13261 ccaccaccag gcaccgctca ggccgataac cacaaacaac accagccagg tgaaggtcat
    13321 cagccagccg tcgcgttttt tgcggcgttt accgagggaa aaactctgca tcggcgcaac
    13381 ttttgcagcc cgtagtggag cctgcttttc cagccctggc agcagttctt cttctggaat
    13441 atgtaccaga cgcgcataag agcggatata tccgcgcagg aatgttgaag caagatcggc
    13501 gggtgcctta tcttcttcaa tgtcgcgtac cgtggaaacc ttcaggcaaa gtcgctcggc
    13561 aacggcctgc tgactaagtc ctagttgttc gcgagcatta cgcaggcgag cgccggtagt
    13621 aagtgcttca ttttggtcgt gcgtggcttc agtattcatt cgctgcaggt acgtttaaat
    13681 gagaattgag atgccggtga accatcatgc tcacccacac cgcgaaacat ccgttaagtt
    13741 aaccgttatc atacagtata agaccgtctg tgtgctcgtg acaaagccca cattcactaa
    13801 ggctaactta ctgttgcatc gttacatact gccttaaagt cagcaaaaac gcaccgttaa
    13861 tattgaccag acaattgcaa cttaatactt cattcataaa acattacgcc acggtacata
    13921 aagtaaccgt ggcgtaatgg ctatcagacc gctttaatgt cgatggcttc accctgcatc
    13981 cgtttacgca gggtacgttt cgtacggtcg ataacatcgc ccgccaactg accacaggca
    14041 gcatcgatat catcaccacg cgttttacgc acaatagtgg tgaaaccgta gctcatcagc
    14101 acttttgaga aacggtcgat acggctgttc gagctgcgtc catacggcgc agccgggaac
    14161 gggttccacg ggatcaggtt gatcttacac ggcgtatctt tcagcagttc cgccagttgg
    14221 tgcgcgtgtt cagtgccgtc gttaacgtgg tcaagcatca cgtattcaat agtgactcgg
    14281 ccctgattgg cgttggattt ctccagataa cggcgcaccg cagcaaggaa cgtttcgata
    14341 ttgtactttt tgttgatcgg cacaatttcg tcacgaattt cgtcgttcgg cgcgtgcagg
    14401 gaaattgcca gcgcaacatc gatcatatcg cccagtttat ccagcgctgg aactacaccg
    14461 gaagtggaaa gcgtcacgcg acgtttagac agaccaaaac cgaaatcatc aagcatgatt
    14521 tccatcgccg gaacgacgtt gttcaggttg agcagcggct cgcccatgcc catcatcact
    14581 acgttagtga ttgggcgctg accggtgact tttgctgcgc cgacgatttt cgccgcacgc
    14641 cacacctggc caataatttc cgatacccgc aggttgcggt taaagccctg ctgggcggtg
    14701 gaacagaatt tacactccag cgcacacccc acctgcgaag agacgcagag cgtagcgcgg
    14761 tcgtcttccg ggatgtacac cgtttcgaca cgctggtcac ccacggcaat cgcccattta
    14821 atggtgccgt cagatgaacg ctgttcttca accacttccg gtgcgcggat ctccgccacc
    14881 tctttcagtt tgccgcgcaa cactttgttg atgtcggtca tctcatcaaa attgtcgcag
    14941 caatagtgat acatccactt catcacctga tcggcgcgga agggtttttc acctaaatct
    15001 ttaaaaaact cccgcatctg ctgacgattg agatccagca ggttgatttt tccatctttg
    15061 gtggtgacgt tttcaggtgt gactaattgt tcagacatat gctattccgg cctcgttatt
    15121 acacgttatg gcccctggag ggttgaaaaa agaaacgccc cggtgagcgg ctgctcgtcc
    15181 gggggcgctg cattgtacaa attctggcgc acggatgcca cgtttgcacg cggcatttac
    15241 gaaattatta acgggtgcgc gggcacactt cgccttcgcc gaagaaataa gcgatttcgc
    15301 gagcggcaga ttcgacggaa tcagaaccgt gggtaccgtt ttcggtcagg ctgtcagcgt
    15361 aatcagcgcg cagagtaccg gccagtgcgt ttgccggatt ggtcgcgccc agcagatcgc
    15421 ggtgacgctg aacggcgttt tcaccttcca gcacggaaac cacgatcggg ccagaggtca
    15481 tgaattcaac cagaccatca aagaacggtt ttccatcgtg ttcagcataa aagccacgtg
    15541 cctgttcaac ggtcaggtgc agcattttgg tgccgacaat tttgaaccct gcagcttcaa
    15601 agcgcgcaaa gatattacca atgacgtttt ttgctaccgc gttcggtttg ataatggaaa
    15661 aagtacgttc aatagccatt tttacctctg taaattgttc tgttgttgtc tgtaccagcg
    15721 tacgagatgg cgcggattat aatgagcaac agagccgttg actattgatg aaggtaacat
    15781 ttttttaaaa taaaatgagt tttagcaaca actcgacttt tatggtgttc tcatcccata
    15841 acgatgacgt tggcaataaa gacaattttg cgcattttgc tgaaaagtaa gaaaggattt
    15901 ttgacaggtt ttacagactt tgcgattagc atgaagattc acaatttttg ccggtagaat
    15961 cgtcaattca acggtcattg cctgatgaaa acaaacagca atacaatttt ggcatccgtt
    16021 acacaaggca tcatttattt gtaaatggcc ttcgttaagc gaaaagacct gttgctcgca
    16081 ggcccgccag catgcaccgc aaagctgaca cttttgagga tccatcatcg ggcgagcatc
    16141 actaatctgg ggccataaat aatgtaaccg acgtaatcct ggttgtattt gtgtcatctg
    16201 aacatccgca cgctgcacat ggaacaaacg acgccgggaa acattcacct cactggtatg
    16261 tggagatacc acagaccagc cttcctcgcc atattgccgt aagcattcgt ttaacagcaa
    16321 taatgcgcgc tcccatgcct tcgcctgact cagatcaatc gcgatcgctg tgatcccctg
    16381 ctctttatgc catagcaaaa gttcttccag cgtcggcgca atagtggtga aaggtgcgac
    16441 cagggtgtga ttgcaaaaat aacgtaaagg ggaattaacg ccacgaatag cctcttgcgg
    16501 acaaataaac tggcaaaccg cacaatcaat acattgttcc gcgtcgaccg ctatgccctc
    16561 ttctgtcagc gttagcgcct gtgccgggca agcctcgaca caggcatgac atgttgcaaa
    16621 acgaaagcgt tggcgcgcgc attccgcgcc aatcgttatg ttatgactta acattagccg
    16681 atactaaaaa agacaaaacg cagcataact tcactcccca ctagcattac gctaccgaat
    16741 aacgccgccg ggatattctt tttacaacca ccgacagcaa agaacatcat cccaatgacg
    16801 gaaattcccc agcttagtgt cagtatgggt tttaactgtc ccagcatcgc taatggcgca
    16861 tgtggcaatg tggctatctg catcgcattt gccgttaaat cccctatcca caacggctga
    16921 accaacaaac gcagcagaac caggagagcg atcaccaaca cgcatccatt acgcagggca
    16981 tcaatctgcg gcaaaattcc gctaatacga aacactgcta ccagcgctga accgagaatt
    17041 cccactgtac ctaagaacat aatcagcgtg ttgtaatgct gccaggtgat aatcgacgta
    17101 tggatgtaaa cttgcgccat gcagaaaaca tcaacaatcc cgaccactgc cgctagcata
    17161 agcaggagtt gccagccgcc tttgcgtaac atgaccagta atgtggataa acccaatagc
    17221 gccagataca gcgcaccaaa gataatttcc cgactcagcc atgaactgga aaagtggctc
    17281 agcgcatgca tcgcattaag tggataaccc aggtgtaagg tagaaatcag taaccctacg
    17341 gcacctgcca aaaaagccaa cagtaatggc ggccgcataa ccgggcgggc gctggaggtg
    17401 attaaaatat gacgatgaag aaacgccagc cacaacgtca taccgaccga tgcctgtaac
    17461 aacagcgtaa aagccattaa cggtaattca tgcatgatgt gacgcctcct tttccgcgcc
    17521 ctgatgcggt ttaatcacca gatttggctg cgtaatcgac gaatcaggta aacctttcac
    17581 atcactgaca tcgccatact tttcacgcaa ctcatcaata gggccgaact taatcgcccc
    17641 caacgggcac gttgccacgc aaatgggttg ttctccttta gcttgcagat caatacagaa
    17701 atcgcatttc gacatctgcc cggtctgctc attcatttgt ggtgcgccgt aaggacagga
    17761 ccaggcgcag taaccgcaac cgacgcactt atcagtattc acgcgcacaa taccgtctcc
    17821 gggacgctta tgcatcgcca tcgtcgggca atttttggta cagatcggat ccgcgcaatg
    17881 gttacaggaa acagaaaggg tataagcaaa gacattatga ctcacaccgc cctctccagt
    17941 ggcgatgaag ccacctccgt tcacttcata cacgcggcga aaacgtcgcc caacctcaag
    18001 gttgtttttg tctttacagg ccacctgaca cgctttacag cccgaacaac gcgaagagtc
    18061 aataaagaac cctaattgtt tgtcgctgac agatgcaaat tctttaaatt ggctcatgct
    18121 ttctcaacct ccaccagcat tgtttgatgc gaattccctt tcgccagcgc cgtaatgcgg
    18181 gtcgaactca gtacattggc acagcctccg cgatcgatac cttgtgcatc cggttgccac
    18241 catgcccccg cctgcatagc aactacgcca gggatgatac ggggggttat ttccaccggg
    18301 ataatgctca caccacgatc gttataaatt tttacgctat ctccttcgct aatcccgcgc
    18361 tgttttgcat cttgcggatt cagccagagt ttttgcgtct gaacttcttg cagccacggg
    18421 ttagcatact gggtggaatt agcacggttt ttccctttcc aggtgatgag ctgcaacggg
    18481 tatttcgccg tcagtttatc ttctggccct tcaaaggcag ggacatagtg cgaaagcgcc
    18541 ggaatttctg gatcttgcat atcaaaaaga cgcttagaga agatctcaat tttgccggaa
    18601 ggcgtcggga aaggattatt ctgcggatcg cgaatatttg cttcgaaagc gatgtgcggc
    18661 gcgcttttga aaagatgtcg acgtgttttt tgcagggttg cgaagtccgg caaattttca
    18721 tccggcatcg ccagacgagt ttgctcccag atatgctcga tccattgctt ctcatcacgt
    18781 ccttgactga actcggcttc gacacccagt tttttcgcca cgtcacgcaa ccagtcataa
    18841 tcggtgcgac gttcaaaatc cggctcaatc agtttttctg acagaataag atagctcgca
    18901 gttccccatg tttcgccgat attccagcgt tccataaagc tggtttccgg cagcaacaca
    18961 tcggcatatt tcgcgctcgg agtcatgaac agatcgctga gtacgatgaa ttctatttta
    19021 gattcgtctt caaggagctt cgcggcctgg tggacgtccg ggttctggtt cgccagataa
    19081 ttacctgcca gtgagaacag caggcggata ttactgtcga gtttatcgac acctttaagc
    19141 ccatcttgcg gcgtaacttt agaagcatca tccgccgcct gcatccagtt cataatcgag
    19201 attttcgctt gtaccggatt ttctggcata tctggtccaa cgcagaattt acgattagaa
    19261 ctaccgccgt aacctgccgc ccagccacct ttgatgccga cgttaccggt aatcgaggcc
    19321 agtaaagttg aacctcgggc ggtgcgttcg ccgcaaatat gtcgctgtgg cccccatccc
    19381 tgaatcaacg ctgcgggttt tgtagtggcg taatcgcgtg ccagttgacg aatggattgt
    19441 gctggaacgt gggtaatttt ttctgcccat tccggcgttt tgtgtatccc atcttttgcg
    19501 ccaaaaaggt aggcaaccaa cgattcgttg gctggtacgc cttccggcat ggagttttca
    19561 tcaaaaccga gagtatatgt gtcgataaat gtcttatcgt ggagattctc gctgataatg
    19621 acgtacatca tcgcgtccat caacgcattg tccgtggtcg gcaacagcgg gatccactga
    19681 tcggcaagtg aagatacggt gtcggaatag cgcggatcca ccacgataaa acgtgtaccg
    19741 ttttgcttca ttttttgaaa atagtaattg gtgtgaccga atatggtttc cgttgggtta
    19801 tgcccccaga gaatcaccag tttggtatcc agaagagtat ccatcgagtt accgctcgcg
    19861 gccacgccat acgtgtaagg cgtg
//
LOCUS       DAZFAB010000083        19512 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZFAB010000083 DAZFAB010000000
VERSION     DAZFAB010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19512
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.083"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(64..440)
                     /gene="rnpB"
                     /locus_tag="ACHJWA_003406"
     ncRNA           complement(64..440)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHJWA_003406"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(473..1618)
                     /gene="garK"
                     /locus_tag="ACHJWA_003407"
     CDS             complement(473..1618)
                     /gene="garK"
                     /locus_tag="ACHJWA_003407"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFO4309938.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYAEVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(1715..2605)
                     /gene="garR"
                     /locus_tag="ACHJWA_003408"
     CDS             complement(1715..2605)
                     /gene="garR"
                     /locus_tag="ACHJWA_003408"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO4309939.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2635..3405)
                     /gene="garL"
                     /locus_tag="ACHJWA_003409"
     CDS             complement(2635..3405)
                     /gene="garL"
                     /locus_tag="ACHJWA_003409"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFO4309940.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3421..4755)
                     /gene="garP"
                     /locus_tag="ACHJWA_003410"
     CDS             complement(3421..4755)
                     /gene="garP"
                     /locus_tag="ACHJWA_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFO4309941.1"
                     /translation="MILDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5130..6701
                     /gene="garD"
                     /locus_tag="ACHJWA_003411"
     CDS             5130..6701
                     /gene="garD"
                     /locus_tag="ACHJWA_003411"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFO4309942.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPKAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            6850..7185
                     /gene="prlF"
                     /locus_tag="ACHJWA_003412"
     CDS             6850..7185
                     /gene="prlF"
                     /locus_tag="ACHJWA_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HFO4309943.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            7185..7649
                     /gene="yhaV"
                     /locus_tag="ACHJWA_003413"
     CDS             7185..7649
                     /gene="yhaV"
                     /locus_tag="ACHJWA_003413"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFO4309944.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH"
     gene            complement(7704..8513)
                     /gene="agaR"
                     /locus_tag="ACHJWA_003414"
     CDS             complement(7704..8513)
                     /gene="agaR"
                     /locus_tag="ACHJWA_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFO4309945.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            8762..10042
                     /gene="kbaZ"
                     /locus_tag="ACHJWA_003415"
     CDS             8762..10042
                     /gene="kbaZ"
                     /locus_tag="ACHJWA_003415"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFO4309946.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKATALSQMVESYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLNRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            10065..10538
                     /gene="agaV"
                     /locus_tag="ACHJWA_003416"
     CDS             10065..10538
                     /gene="agaV"
                     /locus_tag="ACHJWA_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFO4309947.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGIPTEPAVDLFKLL"
     gene            10549..11328
                     /gene="agaW"
                     /locus_tag="ACHJWA_003417"
     CDS             10549..11328
                     /gene="agaW"
                     /locus_tag="ACHJWA_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFO4309948.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            11318..12196
                     /gene="agaE"
                     /locus_tag="ACHJWA_003418"
     CDS             11318..12196
                     /gene="agaE"
                     /locus_tag="ACHJWA_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFO4309949.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            12214..12648
                     /gene="agaF"
                     /locus_tag="ACHJWA_003419"
     CDS             12214..12648
                     /gene="agaF"
                     /locus_tag="ACHJWA_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFO4309950.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            12645..13778
                     /gene="nagA"
                     /locus_tag="ACHJWA_003420"
     CDS             12645..13778
                     /gene="nagA"
                     /locus_tag="ACHJWA_003420"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO4309951.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVSVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLSTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
     gene            14129..15283
                     /gene="agaS"
                     /locus_tag="ACHJWA_003421"
     CDS             14129..15283
                     /gene="agaS"
                     /locus_tag="ACHJWA_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFO4309952.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALHSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFAVL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELHRDNQAMRVIAIAAESSDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            15296..16156
                     /gene="kbaY"
                     /locus_tag="ACHJWA_003422"
     CDS             15296..16156
                     /gene="kbaY"
                     /locus_tag="ACHJWA_003422"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFO4309953.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            16323..16799
                     /gene="agaB"
                     /locus_tag="ACHJWA_003423"
     CDS             16323..16799
                     /gene="agaB"
                     /locus_tag="ACHJWA_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HFO4309954.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDVNVGNMHFSEGKKQISSKVYVDDQDLTDLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            16871..17641
                     /gene="agaC"
                     /locus_tag="ACHJWA_003424"
     CDS             16871..17641
                     /gene="agaC"
                     /locus_tag="ACHJWA_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFO4309955.1"
                     /translation="MAALVFVLGIDFWLEALFLFRPIIVCTLTGAILGDIQTGLITGG
                     LTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLPFSLLMQYVILF
                     FYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCTYLAQGAMQALV
                     KAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVCYIQVSNLLPVA
                     VLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            17631..18422
                     /gene="agaD"
                     /locus_tag="ACHJWA_003425"
     CDS             17631..18422
                     /gene="agaD"
                     /locus_tag="ACHJWA_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFO4309956.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFSLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            18423..19178
                     /gene="agaI"
                     /locus_tag="ACHJWA_003426"
                     /pseudo
     CDS             18423..19178
                     /gene="agaI"
                     /locus_tag="ACHJWA_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417610.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
BASE COUNT         4739 a         5058 c         4951 g         4764 t
ORIGIN      
        1 tttattcatc ttaccctgtg caccaaaagc aaaaacccgc cgaagcgggt ttttacgtaa
       61 atcaggtgaa actgaccgat aagccgggtt ctgtcgtgga cagtcattca tctaggccag
      121 caatcgctca ctggctcaag cagcctaccc gggttcagta cgggccgtac cttatgaacc
      181 cctatttggc cttgctccgg gtggagttta ccgtgccacg gactgttacc agccgcgcgg
      241 tgcgctctta ccgcaccctt tcacccttac ctgatcccgc ttgcgcgggc catcggcggt
      301 ttgctctctg ttgcactggt cgtgggtttc ccccccaggc gttacctggc accctgccct
      361 atggagcccg gactttcctc ccctccgccc gtctcccccg aagaggacga cgacgaagcg
      421 gcgactgtct ggtcagcttc ggcgcgcagt atagagggtt tgcgcgccct tgtcaccccg
      481 cgttgcgcat tccaatcgcc agtgtcgcgg cgatattacg tgaagcacgg cagatattgt
      541 cataagcccc gcggaatgct tcgtccaacg tacctatgct ggtcaatacg ctgaagaccg
      601 catcaatgcc atgctgatgt acaacgccaa catcatcggt caggctaccc gcaatgccaa
      661 tcaccggttt atggtacttc ttcgccacgt ttgcgacacc aatcggtacc ttgccgtgaa
      721 tactctggct gtcaatacgc ccttcaccgg tgatcaccag cgtacaatcg tgaatatgtt
      781 cctccagatt cagcgccgta gtgacgattt caataccact tttcagttcc gcaccaagaa
      841 acgccattag cgccgcgccc ataccacccg cagctcctgc gccggggaca tctttcacat
      901 caacatgcag cgctttttta atgacctcgg catagtgaga gaggttattg tccagctcaa
      961 caatcatcgc ttcactggct cccttttgtg ggccaaagat gcgcgatgcg ccgttatcgc
     1021 ccaccagcgg attggtgaca tcacaagcga cgcgaatgac gcaatctttt aagcgcggat
     1081 cgaggccgga aatatcaata tcattcagag tattaagact accgccgcca aaaccaattt
     1141 cattgccgtt ggcgtcgcat aatttcgccc ccagcgcctg taccatgcct gcgccgccat
     1201 catttgtagc gctgccgcca atgccgataa taatgtttgt cgcgccgctc tccagcgcct
     1261 gcaggattaa ctcgcctgtg ccgcgtgaag tggtcacgag tggatcgcgt ttttccgcag
     1321 gtaccagctc cagcccactg gccgccgcca tttcaataaa cgcggttttg ccatcgccgg
     1381 agatccccca actggcattc actttctcgc ccagcggccc tgtaacccag gcgtgacgtt
     1441 cggaaccctg ggtggctgca atcatcgctt ccaccgttcc ttcgccaccg tcggcaacag
     1501 gaacagaaac gtactgtgca tcaggaaaaa tttcccgaaa tcctttttct atcgcctgcg
     1561 caacctcgct ggcagataaa ctttctttat aagagtctgg ggcgattacg attttcatac
     1621 ctatgcctgt taccacatga cgccggaggg cgtttctctt attcggcctg gattccaggc
     1681 ccggattgca atacgccatc cgggcacgac gtcattaacg agtaacttcg actttcgcca
     1741 gtttttcgta gtagcacgcc agggcgctat gatccgccgt tcctaaacca tctgcacgca
     1801 gtgcctgcat catctccata accgcagctg tgagcggcag ttgtgcgccg acgccgtgag
     1861 aagtatccag cgcattcgcc agatccttaa tatgcagatc aatacggaag cccggcttga
     1921 agttgcggtc catcaccatc ggcgctttgg catccagcac ggtactgccc gccagtccac
     1981 cgcgaattgc ctgataaacc aggtccgggt taacgcccgc tttagttgcc agcgttaacg
     2041 cttctgacat cgcggcaata ttcagcgcca caatgacctg atttgccagt ttggtgacgt
     2101 tacctgcacc gatttccccg gtatgcacca cggaacccgc catcgctttc atcaaatcat
     2161 agtatttgtc gaaaatagcc ttgtcgccgc ctaccatcac tgacagcgta ccgtcgatgg
     2221 ctttcggttc accgccgctc accggagcat ccagcatatc aatgcctttc gctttcagcg
     2281 cttcgctgat ttcacggctt gccagcggtg cgatagaact catatcgatc aataccgtac
     2341 ctggcttcgc gccttcaata atgccattct cacccagcgc cacctctttc acatgagggg
     2401 agtttggcag catggttatg atgacgtcgc actgttcagc gatcgcttta gccgtagaag
     2461 ctgtttctgc acctgcagca atcacgtcag caatagcttc tgggttacgg tcagcaacca
     2521 ccagcgagta acctgctttc agaaggtttt tactcattgg tttacccata atccccaggc
     2581 caataaaacc aactttcata gtcatatcaa tcatctctct tgttgcggtg gtggttattt
     2641 tttaaaggta tcagccagtt tctgagtggc agagcggaag acgccgagat cgctgccgac
     2701 agccacaaac gtcgcgcccc attccagata acgacgcgca tcggcttcga ccggcgcgag
     2761 gataccgctg ggtttgccgt gcgcgctggc acggttaaaa atgtgctgaa ttgctttttg
     2821 tacatccggg tgtgatgcat tgccgagatg gcctaatgcc gcggccagat cgctggggcc
     2881 gacgaagatg ccgtctacgc cttcggtagc ggcaatagca tcgacgttat ctacgccctg
     2941 ctgactttct atctggacca gaatagtgat gttcttgttc gactgagcga aataatccgc
     3001 cacggtgcca aacatattgg cgcggtgaga aacggagacg ccgcgaatgc cttctggcgg
     3061 ataacgcgtc gatgccaccg cctgctctgc ttcctctttt gtttctacaa aaggaatcag
     3121 gaagttatag aaaccgatat ccagaagacg cttaataatt accggctcgt tggtcggcac
     3181 tcgcactact ggcgcgctgg cgctgccttt cagggccatt aactgcggaa taaacgttga
     3241 aatatcgttt ggcgcatgtt cgccatccag caccagccag tcaaacccag ccaaaccaag
     3301 aacttcagtg ctaatcgggt tagagagtgc tgaccagcaa ccaatttgta cctgtttcgc
     3361 agccagtgcg gctttgaatt tattcgggaa aacatcgtta ttcatcgctt atacctttgc
     3421 ttatttctgc aattccatac gtttaatgtc gccaactacg aagaggtagc agaccatcgc
     3481 cattagcgct gaacatccca cgaaaaccag tgctgcattg aaggagtgca gttcacttac
     3541 caggtagcca atcaccagtg gagtgacaat ggaggcaaca ttgccaaaga cgttaaagac
     3601 gccgccgcag aggccaacaa tctctttagg cgcggtgtca gaaatcaccg gccagcccag
     3661 cgcaccaaat cctttaccaa agaaagccag cgccatcagc atgaccacca gcgtggtgtt
     3721 gttggtgtag ttacataaga tgatggtgga agccagcaac attcccagca caatcggtag
     3781 cttacgtgcc agggtcaggg ataaaccgcg tttgatcaga taatccgaga agacacctcc
     3841 cagcacgccg cccgcaaaac cacacagtgc tggaatcgag gcgaccagac ccactttcag
     3901 aatcgacata cctttttcct gcaccagata aatcgggaac caggtgagga agaaccaggt
     3961 gatggtgttg ataaaatatt gtccgaaaaa tacgcccaac atcatgcggt tagagagcaa
     4021 ttgcttgatg taatgcagtt tgggtccgct tgctgccgca ctgcccggct ttttgtggtc
     4081 catatcgacc accgcgccat tttcagagat aaacttcagc tcttccgcag acatacgtgg
     4141 gtgatctgtc gggttatgaa tcaatttgat ccacagcgcc gtcagcacaa aaccaatcac
     4201 ccccataacg gtaaagacgt gctcccagcc ccaggcgaaa gtcagccagc caagcagagg
     4261 cgaaaagagc gccagcgaga aatattgcgc cgagttaaag atggcggagg cagtaccacg
     4321 ttctttcgtc gggaaccagg cggcgacaat acgggcgttc gccgggaatg atggcgcttc
     4381 cgagaagccg agcataaagc gcataaagaa catggagatc cctgcccagg ccagcgggaa
     4441 catatcaaca aagccttgca ggaaggtgaa tagcgaccag aaaaagaggc tgtaggtgta
     4501 aacttttttc gagccaaact tatcaagcag ccagccgccg gggatttgca tcagcaagta
     4561 ggcccagcca aaagcagaaa agatgtaacc catcgaaacc gcacttaact gcaactcttt
     4621 tgccacttcg gtaccagcaa tagacagcgt tgcacgatcg gcgtagttaa cggcggtaac
     4681 aataaaaata atcagtaata ttaaatagcg ggtatgcacg cctttctttt tttcgtcaac
     4741 ggtgtccaga atcattttat ttacctcggg tacttatgct gatttttatt attatgggga
     4801 agatgttatt tatgagtttc atttatgccg taacgacaat gaactcggga attagtataa
     4861 gcagcgcgag aataataatc attgtgcaaa tgctaattta attaatacta tttaaatatt
     4921 attttgagca tatgcacata aggttgcgcg ctaaagcaca gatttgcgct ttaccttgcc
     4981 gggcggcatt gcaatccctg aaatgattga cattgatcac atttctgcgt ttaaactcct
     5041 gacattctta tttcacccaa tgaagtcatt tatttttaaa tgagaccagg tcctcatttt
     5101 aataacctct ggctggagaa tattgcacaa tggccaacat cgaaatcaga caagaaacgc
     5161 caactgcgtt ttatataaaa gttcacgaca cagataatgt ggcaattatt gttaatgata
     5221 atggcctgaa agcaggaacg cgttttccgg atgggctgga attaattgaa catattcccc
     5281 aggggcataa agtcgcattg ctcgacattc cggctaatgg tgaaattatt cgttatggcg
     5341 aagtgattgg ttacgccgtg cgtgcaatcc cacgcggaag ctggatcgac gaatcaatgg
     5401 ttgtactgcc gaaagcgccg ccgttacaca cgctgccact ggcaaccaaa gtcccggaac
     5461 ccttaccgcc gctggaagga tacacctttg agggctatcg caatgccgat ggcagcgtgg
     5521 gcaccaaaaa cctgctcggc atcaccacca gcgtccactg tgtggcaggc gtggtggact
     5581 acgtagtaaa aatcattgaa cgcgatctgc taccgaaata cccgaacgtc gatggcgtgg
     5641 tggggctgaa tcatttgtac ggttgtggcg tggcgattaa cgcaccggcg gcagttgtac
     5701 ctatccgtac cattcacaat atttcgctga atcctaactt tggcggcgaa gtaatggtga
     5761 ttggcctggg ttgtgaaaag ttgcagcctg agcgcctgct gactggaacg gatgatgtgc
     5821 aagctattcc agtagaaagc gccagcattg tcagtttgca ggatgaaaag catgtcggtt
     5881 ttcagtccat ggtcgaggat attttgcagg tcgccgaacg ccatctacaa aaactgaatc
     5941 aacgtcagcg agaaacctgt ccggcttcag aactggttgt cggcatgcag tgcggtggca
     6001 gcgatgcctt ttctggcgta acggcaaacc cggcggttgg gtatgcgtct gatctactgg
     6061 tgcgctgcgg cgcaacggtg atgttctcag aagtcacgga agtgcgtgac gcgatccatc
     6121 tgctgacacc acgcgcagtg aacgaagagg tcggcaaacg gctgctggag gagatggagt
     6181 ggtacgataa ctatctcaat atgggaaaaa ccgaccgcag cgccaaccct tcgccgggca
     6241 acaagaaagg cggtctggca aatgtagtgg agaaagcgct cggctccatt gctaaatcgg
     6301 gtaaaagtgc aattgttgaa gtgctgtcgc ccggtcaacg cccgactaaa cgcggattaa
     6361 tttacgccgc gacgccagcc agcgattttg tctgtggcac gcaacaggtg gcttcgggta
     6421 tcaccgtgca agtgtttacg accggtcgtg gtacgccgta cggcctgatg gcggtacccg
     6481 tcattaaaat ggcgacccgc accgagctgg cgaaccgctg gtttgattta atggatatta
     6541 acgcgggcac tatcgccacc ggcgaagaaa ccattgaaga ggtgggctgg aagttgttcc
     6601 actttattct cgacgtcgcc agcgggaaga agaaaacctt ctcggatcaa tggggactgc
     6661 ataaccagct ggcggtgttt aacccagcac cggtgacctg atttcttttg gaacgtccct
     6721 cgcaaaacat ggccttagtg ccatgttttt attgtttaaa gcccccacgt ccattaataa
     6781 tgcatttgca ttaccttagt tcaagcttat aatttgagca gaaaacaggc tgtaaaagga
     6841 cagtgaatca tgcccgctaa tgctcgctct cacgctgtac tgaccactga atcaaaggtc
     6901 acgatacgcg gacaaacaac tatccccgcg ccagtgcgtg aggccttaaa actgaagcca
     6961 ggccaggaca gcattcatta cgaaattctg cctggtgggc aagtatttat gtgccgactg
     7021 ggagatgagc aggaggatca tactatgaat gcatttttgc gttttctgga tacagatatc
     7081 cagaacaacc cgcaaaaaac tcgtccattc aacattcaac aaggaaagaa acttgtcgct
     7141 ggcatggacg tcaacattga tgatgagatt ggcgacgacg aataatggat tttccacaaa
     7201 gggttaatgg ttgggcgcta tatgctcatc cctgttttca ggaaacctac gacgctttag
     7261 ttgccgaagt cgagacatta aagggaaaag atcctgaaaa ttatcagaga aaagctgcca
     7321 caaagttatt ggcggtggtc cataaagtga ttgaggagca tatcacggtc aatccatcat
     7381 caccggcatt ccgtcatggc aagtcgttag gctctgggaa aaataaagac tggtcacggg
     7441 taaaatttgg tgctggtcgc tatcgtctct tctttcgtta cagtgaaaaa gagaaagtca
     7501 tcattctggg atggatgaac gatgaaaaca cgctgcgcac ctacggtaaa aaaacagatg
     7561 cctataccgt attcagcaaa atgttaaaaa gaggacatcc tcctgccgac tgggaaaccc
     7621 tcacccgaga gacagaagaa acccattgat ggtgttcaca atgcccgccg aacgcattcg
     7681 tgttgaccag cgggcaccct ttctcactcc ccgaccagaa tcacttcaac cccagccttt
     7741 cgcagccctt ccaggctatc cgcaggaatg ccttcatcaa caatgatcat gtcgatacgt
     7801 tgagtatcaa tgatcttatg taaactggaa cgattgaatt tactggaatc ggtgaccacg
     7861 atgatccgtt ccgcaacttc gcacatccgg cggtttaaac gggcttcatc ttcattatgc
     7921 gtgctgacgc cgcgctccag atcgatcgca tctacaccaa gaaacagcat atcgaagtgg
     7981 taattttgca gcgattgctc agcctgatcg ccgtaaaaag attgcgactg acggcgcaaa
     8041 tgcccgccgg tcatcagcag ctcaacgcct tccgcttcca gcaacgcatt agccacgttc
     8101 ataccgttgg tcatcgcaat tacgtcagtg tgcttgcgca tcagacgagc aatctcaaaa
     8161 gtggtggtcc cggaatcgag gatcacccga tgacctggct gaatcaactc aacggcagct
     8221 ttcgcaacgc tgcgtttcat tgcggtgttc agcgcgcttt tatcttccac tgatggctcg
     8281 actgacggcg ttgtgctatc gcagatcaac gcgccaccat aggcacgcac agcgatcccc
     8341 tgcttttcca gaaacgccag atcgttgcgg atcgtcacgg tagatacgcc atacaatgcc
     8401 gacagatcgt taacctgcac actcccttgc tgtcgcagac gctgaatgat ctgttctcgt
     8461 cgctcgctgg tgcctgtcac tcgcttctca cctgaagcgt cggtattact catagtaagt
     8521 cctttcgtaa aactttcgtt tcatttcgtt ttgcctatta acgcctttct attaagcaaa
     8581 tgcaagccca ccttgcccat tgacgcaagc tactctcgtt tcagtgactt tcattatgtt
     8641 tcttttgtga atcagatcag aaaactatta tctttcgttt tatttttatc tcaccatgac
     8701 gcagtatcaa ctgaaacaaa acgaaagatt aatatcgcag taatctgaac tggagaggaa
     8761 agtgaaacat ctgacagaaa tggtgagaca gcacaaagcg ggcaaaacaa atggaattta
     8821 tgccgtttgt tccgcacatc cgctggtgct ggaggctgca atccgctacg ccagtgcaaa
     8881 ccaaacgccg ttactgattg aagcaacctc caatcaggta gaccagttcg gcggttatac
     8941 cggaatgacg cccgccgatt ttcgcggctt tgtttgtcag ctcgccgact cgttgaattt
     9001 cccgcaggat gcgttgattc tgggtggtga ccatctgggg ccaaaccgct ggcaaaacct
     9061 gccggccgct caggcaatgg ccaatgccga tgatttgatt aaaagctacg ttgcggcagg
     9121 attcaaaaaa atccaccttg attgcagcat gtcctgtcag gacgatccga ttcccttaac
     9181 tgatgacatc gtggctgaac gcgccgcccg tctggcgaaa gtggcggaag aaacctgtct
     9241 tgaacacttt ggcgaagccg atctggagta tgtcattggt accgaagtgc cggtacctgg
     9301 cggcgcgcat gaaaccttaa gcgagctggc ggtcaccacg ccggatgccg cccgcgccac
     9361 gctggaagcc catcgtcacg cctttgaaaa gcaaggtttg aatgccatct ggccacgcat
     9421 cattgccctg gtggttcaac ccggcgtcga attcgatcac accaacgtta ttgattatca
     9481 gcccgccaaa gcgaccgcct taagccagat ggtcgaaagc tacgaaacgc tgattttcga
     9541 agcgcactct accgattatc aaacgccgca atcgctgcgc cagctggtga ttgaccactt
     9601 tgccattctg aaagttggcc cggcgctgac cttcgccctg cgtgaagctc tgttctctct
     9661 ggcagcgatt gaagaagaac tggtgccagc aaaagcctgt tctggtctgc gtcaggtgct
     9721 ggaaaacgtg atgctcaacc gcccggaata ctggcaaagc cactaccacg gtgacggcaa
     9781 cgcgcgtcgt ctggcgcgtg gttatagcta ctcggatcgc gtgcgctatt actggccgga
     9841 cagccagatt gatgacgctt tcgctcatct ggtacgtaat ctggcggatt caccaattcc
     9901 gctgccgctg atcagccagt atctgccgct gcagtacgtg aaagttcgct ccggcgagct
     9961 gcagccaacg ccacgggaac tcattatcaa ccatattcag gacatcctgg cgcagtacca
    10021 cacagcctgt gaaggccaat aagcaaaaca aagaggaaca cgctatgcca aatattgttt
    10081 taagccggat tgatgaacgc ttgattcacg gtcaggtcgg cgttcagtgg gtcggatttg
    10141 cgggggcaaa tctggtgctg gtcgccaacg atgaggttgc cgaagatccg gtacaacaaa
    10201 acctgatgga aatggtactg gcagaaggga tcgccgtacg tttctggacg ctgcaaaaag
    10261 ttatcgacaa cattcatcgc gccgccgatc gacagaaaat cctgctggtt tgtaaaacac
    10321 ccgccgattt cctgacgctg gtgaaaggtg gcgttccggt gaatcgcatt aacgttggca
    10381 atatgcacta cgccaatggc aagcaacaaa tcgccaaaac ggtttctgtg gatgcgggcg
    10441 atatcgcagc atttaacgac ctgaaagccg ctggggtgga atgcttcgtt cagggcatcc
    10501 cgacagagcc tgctgtggac ctctttaaat tactttgagg gattcatcat ggaaatcagt
    10561 ctgttgcagg cattcgcgtt gggcattatc gcctttatcg ctggcctgga tatgtttaac
    10621 ggcttaaccc atatgcaccg cccagtggtc ctcggcccgt tagtcgggct ggtacttggc
    10681 gatctgcata ccggtatttt aaccggcggt acgctggaac tggtgtggat ggggctggcc
    10741 ccgctggcgg gcgcacagcc gcctaatgtg attatcggta ctatcgtcgg cacggcgttt
    10801 gccattacta ctggcgtgaa acccgatgtc gcagtcggtg tcgccgtacc tttcgctgtc
    10861 gcagtacaga tggggattac cttcctgttc tcggtgatgt ctggcgtgat gtctcgctgc
    10921 gaccggatgg cggagaacgc cgatactcgc ggcattgaac gcgtaaacta tctggcactg
    10981 ctggcactcg gcactttcta ttttctctgc gctttcctgc ctatctactt cggcgcggaa
    11041 catgcaaaaa ccatcattga tgtcctgccg caacgattaa tcgacggcct cggcgtcgcg
    11101 ggcggcatca tgccagcaat cggttttgcc gtgctgctga aaatcatgat gaaaaacgtc
    11161 tacatcccct acttcatcct gggctttgtt gccgcagcct ggctcaagtt accggtgctg
    11221 gctattgctg ccgccgcgct ggcgatggcg ctgatcgatc tgctgcgtaa atctcctgaa
    11281 ccgactcaac ctgcggcaca gaaagaggaa tttgaagatg gcatctaatc aaaccaccct
    11341 gccgaacgtc agtgaaaacg aagaaacatt gctgactggc gtcaatgaaa acgtgtatga
    11401 agatcagagc attggcgcgg agctgacgaa aaaagatatc aatcgcgtcg cctggcgttc
    11461 catgctgtta caagcgtctt ttaactacga acgtatgcag gcttccggct ggttgtacgg
    11521 tctgctgcct gctctgaaaa agatccacac taataaacgc gacctggcgc gcgccatgaa
    11581 ggggcatatg ggtttcttca atacccatcc gtttctggtg acatttgtta tcggcattat
    11641 ccttgcgatg gagcgttcta agcaggacgt taacagtatt cagagcacca aaattgccgt
    11701 cggtgcgccg ctcggcggga ttggcgatgc aatgttctgg ctaacgctac tgccgatttg
    11761 tggcgggatt ggagccagtc tggcattgca aggctccatt cttggcgcag tcgtctttat
    11821 tgtgctgttc aacgtggtcc acctggggct gcgttttggt ctggcgcatt atgcttaccg
    11881 catgggcgtg gcggcgattc cactcattaa agctaatacc aaaaaagtcg gccatgctgc
    11941 gtctatcgtt gggatgacgg taatcggtgc gctggtggca acctatgttc gtttaagcac
    12001 cacgctggaa atcacagcgg gcgacgcagt ggttaagtta caggctgatg ttatcgacaa
    12061 actgatgcct gccttcttac cgttggtcta caccctgacc atgttttggt tggtacgccg
    12121 cggctggagt ccgctgcgtc tgattgcagt taccgtggtt ctcggcatcg ttggtaaatt
    12181 ctgccatttc ctttaaatac aatgaggtat tagatgttaa gtattatttt gacagggcat
    12241 ggcgggtttg ccagcggcat ggaaaaagca atgaagcaaa ttctcggtga gcaatcgcag
    12301 tttatcgcca tcgattttcc ggaaacctca agcaccgcgc tgcttacatc gcagcttgaa
    12361 gaggccatcg cccaactgga ttgtgaagac ggcattgttt ttctgacgga tttgctgggc
    12421 ggcacgccgt ttcgcgtagc ttcaacgctg gcgatgcaaa aaccgggctg tgaagtgatc
    12481 accggcacca atttgcaact gctactggag atggtgctgg agcgcgaagg gttaagcggt
    12541 gaagaattcc gtgtacaggc gctggagtgt ggacatcgtg ggctgaccag tctggtggac
    12601 gagttaggcc gctgccatga agaatgtccg gtcgaggaag ggatatgaca cacgttctgc
    12661 gcgccagaag gctgctgact gaagagggat ggctcgatga ccatcagttg cgtattgctg
    12721 acggtgtcat cgcagcaatc gaaccgattc cagtgagcgt gactgaacgc gatgcggaac
    12781 tgctctgccc cgcttacatc gacacccatg tacacggtgg tgcgggcgtt gatgtaatgg
    12841 atgacgcgcc ggatgtactc gacaagctgg caatgcacaa ggcacgcgaa ggtgtcggca
    12901 gttggttgcc gactaccgta accgcgccgc ttagtaccat tcatgcggcg ctgaaacgta
    12961 ttgctcaacg ttgccaacgc ggcggacctg gtgcgcaagt gctggggagt tatctcgaag
    13021 gaccgtactt cacgccgcag aataaaggcg cgcatccgcc ggagttgttt cgcgagcttg
    13081 aaattgccga gctggatcaa ttgattgccg tttctcagca caccttacgc gtggtagcgc
    13141 tggcaccgga aaaagagggg gcattgcagg ccatccgcca tcttaaacag caaaatgtac
    13201 gagtgatgct ggggcatagc gcggcgacct ggcaacaaac tcgcgccgcg tttgatgctg
    13261 gtgccgacgg cctggtgcat tgctataacg ggatgacagg tttacatcac cgcgaaccgg
    13321 gaatggttgg cgcgggatta acggacaagc gcgcctggct ggaactgata gccgatggtc
    13381 atcatgtgca tccggcggca atgtcgctgt gttgttgctg tgcgaaagag agaatcgtac
    13441 tgatcaccga cgcgatgcag gcagccggga tgccggatgg tcgctatacg ttatgtggcg
    13501 aagaagtgca gatgcacggt ggcgttgtcc gtaccgcgtc cggtgggctg gcgggcagta
    13561 cgctgtctgt tgatgcggca gtgcgcaaca tggtcgagtt gacgggcgta acgcctgcgg
    13621 aagccattca tatggcgtcg ctgcatccgg cgcgaatgct gggtgttgat ggtgttctgg
    13681 gatcgcttaa accgggcaaa cgcgccagcg tcgttgcgct ggatagcggg ctgcatgtgc
    13741 aacaaatctg gattcagggt caattagctt cgttttgatg gtttgctcca ttattgggcc
    13801 ttcacttccc ccgtaaggcc tttctttttc tttcgttttg atctgtgcag cggtgtcgga
    13861 tgcgacgcta acgcgtctta tccgacctac agttggtgac cgcaaggccg gataaagcgt
    13921 ttgcgccgca tccagcaatc ccttttgctt cctttatctt ttctttcaac gatcacggat
    13981 ttcgttttat ttcctttttc tccattgaac tttcggtttc ttttctatag attttaatca
    14041 acgaaagaca tcaccaagtg aaatgaaacg aaaggcaagt gaaagcgaca acgcccgacg
    14101 tcaagttcat cagactaagg attgagttat gccagaaaat tacacccctg ctgccgccgc
    14161 aaccggtaca tggactgaag aagagatccg ccatcagcct cgcgcatgga tccgttcgct
    14221 caccaacatc gacgcgctac attccgcgct taataacttc cttgaaccgt tactgcgcaa
    14281 agagaatctg cggatcatcc tgaccggagc cggaacctcg gcatttatcg gtgacatcat
    14341 cgcgccgtgg ctcgccagcc ataccggtaa aaacttcagc gccgtaccga ccaccgatct
    14401 ggtcactaat ccgatggact acctgaaccc agcccatccg ctgctgttga tctccttcgg
    14461 tcgatccggc aacagcccgg aaagcgtcgc agccgtggaa ctggcaaatc aatttgtacc
    14521 ggaatgctat cacctgccga tcacctgcaa cgaagcgggc gctctttacc aaaacgcgat
    14581 caacagcgat aacgcgtttg ccgtgctgat gcccgcagaa acgcacgatc gcggctttgc
    14641 gatgaccagc agcattacca ccatgatggc cagctgcctc gcggttttcg cacctgagac
    14701 gatcaacagc caaaccttcc gcgacgtggc ggatcgttgc caggcgatcc tgacctcact
    14761 gggcgatttc agcgaaggtg tgtttggtta cgcaccgtgg aaacggatcg tttatctcgg
    14821 cagcggtggc ttacagggcg cagcacgcga gtcggcgctg aaagtgctgg aactgacggc
    14881 gggtaaactg gcggcctttt atgattctcc aaccggattc cgtcatggac caaaatcgct
    14941 ggtcgataac gaaacactgg tggtggtatt tgtctccagc cacccttaca cccgtcagta
    15001 tgatcttgat ctgctggctg aacttcaccg tgacaaccag gcaatgcgtg taatcgccat
    15061 cgccgcggaa agcagcgaca tcgtcgctgc cggtccacat atcatcctgc caccgtcacg
    15121 tcactttatc gacgttgagc aggcattttg cttcctgatg tacgcccaga cgtttgcact
    15181 gatgcagtcg ctgcacatgg gcaatacgcc ggatacccca tcagccagtg gcaccgttaa
    15241 ccgcgtggtg caaggcgtaa tcattcatcc gtggcaggca taagaggatc gcattatgag
    15301 cattatctcc accaaatatc tgttacagga cgcccaggcc aatggctacg cggtgcctgc
    15361 ttttaacatt cataacgccg agacgatcca ggcgatcctc gaagtgtgca gtgaaatgcg
    15421 atcgccggtg atcctcgccg gaacgccggg gacctttaaa catatcgcgc tggaagagat
    15481 ctacgccctg tgcagcgcct attccacaac ctacaacatg ccactggcgc tgcatctcga
    15541 ccaccacgaa tcgctggatg atattcgccg taaagtccac gcaggtgtgc gcagtgcgat
    15601 gatcgacggc agccacttcc cgtttgccga gaacgtaaag ctggtgaaat cggttgtcga
    15661 cttctgccac tcccaggatt gcagcgtgga agcagaactg ggccgcctgg gcggtgttga
    15721 agatgacatg agcgttgacg ccgaaagtgc attcctgacc gatccacaag aagctaaacg
    15781 ctttgtcgaa ctgactggcg tcgacagctt ggcggtagcg attggtacgg cgcacggctt
    15841 atacagcaaa acgccgaaga ttgatttcca gcggctggcg gaaattcgtg aagtggtgga
    15901 tgttcctctg gtgctacatg gtgccagcga tgttccggat gaatttgtcc gtcgcactat
    15961 tgaacttggc gtcacaaaag tgaacgttgc cacagaatta aaaatagcct tcgccggcgc
    16021 ggttaaagcc tggtttgcgg aaaatccgca gggtaatgat cctcgttatt atatgcgcgt
    16081 cggaatggat gcgatgaaag aagttgtcag aaataaaatt aatgtctgtg gttcagcgaa
    16141 tcgaatttca gcataatcat tagtttttta tgatttatcc caatgtactt cccgattaat
    16201 atcggggagt gccttaatgg aaaaggagat gactaaacct taataaatag atcagtacaa
    16261 tatcgcaaca ataatatatt taaaaaaatt atattattca actttatggt gaggattaca
    16321 caatgagcag tccaaatatt ctcttaaccc gtattgataa ccgtctggtt cacggtcagg
    16381 ttggcgtgac ctggacatcc accatcggtg cgaatctgct ggtagtcgtg gatgatgttg
    16441 tcgctaacga tgatattcaa cagaaattaa tgggtattac cgcagaaacc tacggctttg
    16501 gcattcgttt ctttactatc gaaaaaacca ttaacgtcat cggcaaagct gcaccgcatc
    16561 agaagatctt cctgatttgc cgtacgccac aaacggtacg taaattggta gaaggtggca
    16621 ttgacctgaa agatgtcaac gtcggcaata tgcatttctc ggaagggaaa aagcaaatca
    16681 gcagtaaagt ttatgtcgat gaccaggatc tcacggactt acgttttatt aaacaacgtg
    16741 gcgtgaatgt tttcattcag gacgtccctg gcgatcaaaa agaacaaatc cctgactaaa
    16801 tctaaaatcg ccttaatatt ggtttgaggt aataaaaatg catgaaataa ccctacttta
    16861 gggattatcc ctggcggcgt tagttttcgt tctggggatt gatttttggc tggaagcctt
    16921 atttttattc cgcccgataa tcgtttgtac cctaactggc gctattctcg gtgatattca
    16981 gactggctta attaccggtg gtctgacaga gttggctttc gccggattaa cccctgcagg
    17041 tggtgttcag ccgcccaacc cgattatggc gggtctgatg accaccgtca ttgcatggtc
    17101 tacgggcgtt gatgccaaaa cggcaattgg tcttggcctg ccgtttagtt tgttaatgca
    17161 gtacgtcatt ctgttcttct attctgcttt ctcattattt atgaccaaag ccgataaatg
    17221 cgcgaaagag gcggatacgg cagcgttttc ccgacttaac tggacaacga tgctcatcgt
    17281 cgcttcagcg tatgcggtga ttgctttcct ctgtacttac ctggcacaag gggcgatgca
    17341 ggcgctggtg aaagcgatgc ccgcttggct gacccacggc tttgaagtgg ctggcggtat
    17401 tctgcctgcc gttggttttg gcttgctgct gcgcgtgatg ttcaaagcac aatatatccc
    17461 ttacctgatc gccggcttcc tgtttgtttg ctacatccag gtcagcaacc tgttgccggt
    17521 tgccgtactg ggcgcaggct ttgcggtgta tgagtttttc aatgcgaaat cccggcagca
    17581 agcgcaaccg cagcccgttg ccagtaaaaa tgaagaagag gactacagca atgggatctg
    17641 aaatcagtaa aaaagatatc acccgtctgg gctttcgttc gtcgctgctg caagcgagct
    17701 ttaactacga aaggatgcaa gcgggcggtt ttacctgggc gatgttgccg atcctgaaaa
    17761 agatttataa ggatgacaaa ccgggcctaa gcgcagcgat gaaagataac ctcgaattta
    17821 ttaataccca cccgaatctg gtcggattcc tgatggggtt attaatttcg atggaagaaa
    17881 aaggagaaaa ccgcgacacc attaaaggcc tcaaagtggc actgtttggc ccaatcgccg
    17941 ggattggcga tgcgattttc tggtttactt tgttgccgat tatggcggga atttgctcat
    18001 catttgccag ccagggaaac ctgctggggc cgattctatt tttcgccgtt tacctgctta
    18061 tcttttccct gcgcgtcggc tggacccacg tcggttattc agtcggcgtg aaggcgatcg
    18121 ataaagtgcg agagaactcg cagatgattg ctcgctcggc aaccatcctc gggatcacgg
    18181 taatcggcgg gctgatcgct tcgtatgtgc atattaacgt ggtgacatcg tttgccatcg
    18241 acagtaccca cagcgtcgcg ctgcaacagg atttcttcga taaagtcttc ccgaacattt
    18301 taccgatggc ctacaccctg ctgatgtatt acttcctgcg ggtgaaaaaa gcgcatccgg
    18361 tgctgttaat cggcgtgact tttgtgctct ctattgtttg ttccgcattc ggcattttgt
    18421 aaatggaacg aggcactgcg tctggcggtg cctctttact taaggaattt catcctgtgc
    18481 aaacccttca gcaagttgaa aactatacgg cgttaagtga acgtgccagc gaatatttat
    18541 tggccgtgat ccgtagcaaa ccggatgccg tgatttgcct ggcgaccgga gccacgccat
    18601 tactgacgta tcattatctg gtagaaaaaa tccactagca gcaggttgat gtcagccagc
    18661 tcaccttcgt gaagctcgac gaatgggtgg atctgccatt aacgatgcct ggtacctgcg
    18721 aaactttcct gcaacagcat atcgtgcagc cgctggggct acgtgaagac cagctcatca
    18781 gctttcgctc cgaagagata aatgagacag agtgcgaacg ggtaacgaac ctgattgcgc
    18841 gcaaaggcgg tctggattta tgcgttctcg gattggggaa aaacggtcat cttgggctga
    18901 acgaaccggg agaaaacctg caaccggcct gccatatcag tcaacttgat gccagaacac
    18961 agcaacatga gatgttaaaa accgcgggtc gccccgtgac tcgtgggatc accttaggcc
    19021 tgaaggatat tctcaatgcc cgcgaggttt tgttactggt gactggcgaa ggaaagcagg
    19081 atgcgacaga acgttttctc acggctaaag tctctacctc tatcccggct tcgtttttgt
    19141 ggctgcacag taactttatt tgtttaatta atacctaacc agatgaaaaa tttgctcaaa
    19201 atgtttaaat taacttatgc aacagtcacg cattatatta aataacattt gactgggttg
    19261 aacataacgc caatagcaaa aggagttatg ttcaacaatt taattttaga tatacatata
    19321 acttgttcta ttaataaacc atctcgtatt ctgactcgcc ccctggcgca gcaaagtcat
    19381 gcacttatac tcttttgaat acatagaaaa ttgatatcaa tataatgaaa atatcaaata
    19441 tttgcatata aatataatct taaagtttag tctatttaat gttcaatgaa atatttctac
    19501 ctgtataatc tt
//
LOCUS       DAZFAB010000084        19351 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZFAB010000084 DAZFAB010000000
VERSION     DAZFAB010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19351
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.084"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            27..929
                     /locus_tag="ACHJWA_003427"
     CDS             27..929
                     /locus_tag="ACHJWA_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4309957.1"
                     /translation="MTNLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLRGLEQTINIVNKSELKKNFII
                     HGPQLISCSNNSMLIRCLRQDSSVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNRHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDER
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNECF"
     gene            1076..2296
                     /locus_tag="ACHJWA_003428"
     CDS             1076..2296
                     /locus_tag="ACHJWA_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO4309958.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQSLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            2269..2898
                     /locus_tag="ACHJWA_003429"
     CDS             2269..2898
                     /locus_tag="ACHJWA_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFO4309959.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRINAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(2899..4059)
                     /gene="ybdL"
                     /locus_tag="ACHJWA_003430"
     CDS             complement(2899..4059)
                     /gene="ybdL"
                     /locus_tag="ACHJWA_003430"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFO4309960.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQASAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     IADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTREHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            4168..5256
                     /gene="hcxA"
                     /locus_tag="ACHJWA_003431"
     CDS             4168..5256
                     /gene="hcxA"
                     /locus_tag="ACHJWA_003431"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFO4309961.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGVR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(5266..5463)
                     /locus_tag="ACHJWA_003432"
     CDS             complement(5266..5463)
                     /locus_tag="ACHJWA_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO4309962.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(5645..7750)
                     /gene="cstA"
                     /locus_tag="ACHJWA_003433"
     CDS             complement(5645..7750)
                     /gene="cstA"
                     /locus_tag="ACHJWA_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFO4309963.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKEPKPTAKETP
                     YEPMPENVEEIVVQAKGAH"
     gene            complement(7931..8344)
                     /gene="entH"
                     /locus_tag="ACHJWA_003434"
     CDS             complement(7931..8344)
                     /gene="entH"
                     /locus_tag="ACHJWA_003434"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFO4309964.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDI
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(8347..9093)
                     /gene="entA"
                     /locus_tag="ACHJWA_003435"
     CDS             complement(8347..9093)
                     /gene="entA"
                     /locus_tag="ACHJWA_003435"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFO4309965.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(9093..9950)
                     /gene="entB"
                     /locus_tag="ACHJWA_003436"
     CDS             complement(9093..9950)
                     /gene="entB"
                     /locus_tag="ACHJWA_003436"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFO4309966.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(9964..11574)
                     /gene="entE"
                     /locus_tag="ACHJWA_003437"
     CDS             complement(9964..11574)
                     /gene="entE"
                     /locus_tag="ACHJWA_003437"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFO4309967.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLLNDSGE
                     HNLQDAINHPADGFTATPSPADEVVYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQINVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(11584..12759)
                     /gene="entC"
                     /locus_tag="ACHJWA_003438"
     CDS             complement(11584..12759)
                     /gene="entC"
                     /locus_tag="ACHJWA_003438"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFO4309968.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12948..13904
                     /gene="fepB"
                     /locus_tag="ACHJWA_003439"
     CDS             12948..13904
                     /gene="fepB"
                     /locus_tag="ACHJWA_003439"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO4309969.1"
                     /translation="MRLAPLYRNALLLTGLLFSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSTEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQALLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(13908..15158)
                     /gene="entS"
                     /locus_tag="ACHJWA_003440"
     CDS             complement(13908..15158)
                     /gene="entS"
                     /locus_tag="ACHJWA_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFO4309970.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDG"
     gene            15269..16273
                     /gene="fepD"
                     /locus_tag="ACHJWA_003441"
     CDS             15269..16273
                     /gene="fepD"
                     /locus_tag="ACHJWA_003441"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFO4309971.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            16270..17262
                     /gene="fepG"
                     /locus_tag="ACHJWA_003442"
     CDS             16270..17262
                     /gene="fepG"
                     /locus_tag="ACHJWA_003442"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFO4309972.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            17259..18074
                     /gene="fepC"
                     /locus_tag="ACHJWA_003443"
     CDS             17259..18074
                     /gene="fepC"
                     /locus_tag="ACHJWA_003443"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO4309973.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTLGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(18071..19204)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHJWA_003444"
     CDS             complement(18071..19204)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHJWA_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFO4309974.1"
                     /translation="MSSLNIKQGSDAHFPDYPLASPSNNEIDLLNLISVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSEEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKMKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANINDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
BASE COUNT         4653 a         5415 c         4886 g         4397 t
ORIGIN      
        1 gaagaaaaat ataaccggag tagtgtatga ccaatctcta cgacttgaaa aagttcgact
       61 taaatcttct tgtcatattt gagtgtattt accagcattt aagcattagc aaagctgctg
      121 aatcactata tatcacccca tcagccgtga gccagtcgtt acaacgctta cgtgcgcaat
      181 ttaatgaccc tttattcatt cgctcaggaa aaggtatcgc gccgacgaca acagggttaa
      241 atctgcatca ccatcttgag aaaaatctca gaggccttga acaaacaatt aatatagtca
      301 ataagtccga actaaagaaa aactttatta ttcacggccc tcagctcatt tcttgctcta
      361 ataatagtat gctgatccgc tgcctacggc aagattcctc tgtcgagatt gagtgccatg
      421 atatcctcat gtcagcagag aatgctgaag aactgttggt tcaccgcaaa gccgatctgg
      481 tgatcaccca aatgccagtg atcagccgtt ccgtcatttg catgccattg catacgattc
      541 gcaatactct aatttgtagt aataggcatc cacgaattac tgacaatagc acttatgaac
      601 aaattatggc agaagagttc acccaattaa tatctaaatc agcaggggtt gatgatattc
      661 aaatggaaat agacgagaga tttatgaatc gcaaaatttc atttcgtggt tcctctttgc
      721 tgacaatcat taatagcatt gcagttacag atttactggg aatagtcccc tatgaacttt
      781 ataactccca ccgtgacttt cttaatttaa aagagataaa actagaacat ccgttacctt
      841 caataaagct ttacatttcc tataataaat cttctttaaa caatctggtg ttctcacgat
      901 ttattgaccg tctaaatgag tgtttttgat atataacctc agacagctac tataactttc
      961 acctatttat tcacagcgcg aatgaaaaat aaaacactct gcttttcagt gtttacttta
     1021 taattaacag tcttatctca ctgatcaccc tgtaacgttc agagagcgtc ttcagatgtc
     1081 tatttataaa attcctcttc cgctcaatat tctggaagct gcacgagagc gtattacctg
     1141 gacactcaac accctacccc gcgtttgtgt ctcattttct ggcggcaaag actctggttt
     1201 aatgctgcat ctgacagccg aactcgcccg acaaatgggc aaaaaaatct gcgttttgtt
     1261 tatcgactgg gaggcgcaat tctcttgcac cattaactat gttcagtccc tgcgcgagtt
     1321 gtacaccgat gtcatcgaag agttttactg ggttgcgctc ccgcttacga cgcaaaattc
     1381 cctttcacaa taccaacccg aatggcagtg ctgggaacct gatgtcgaat gggtacgtca
     1441 gcccccgcaa gatgcgataa ccgatcctga ctttttctgc ttttaccagc caggcatgac
     1501 cttcgaacaa tttgtacgtg agtttgccga atggttttca caaaaacgtc cggcggcgat
     1561 gatgatcggc atccgtgcgg atgagtccta caaccgtttt gtcgccatcg ccagtttaaa
     1621 taaacaacgt tttgccgacg ataaaccctg gactacggcc gcaccaggcg gtcatagctg
     1681 gtacatttac cccatttacg actggaaagt ggctgatatc tggacctggt atgcaaatca
     1741 tcaaagtctc tgcaacccac tgtataacct aatgtatcag gcaggcgttc ctctgcgtca
     1801 tatgcgaatt tgcgaacctt ttggcccgga gcaacgacaa ggattatggc tctatcacgt
     1861 tatcgaaccg gatcgctggg ctgctatgtg cgcacgagtc agcggggtaa aaagtggcgg
     1921 catttacgcc ggacatgata atcatttcta tgggcatcgg aaaatcctca agccagaaca
     1981 tttagactgg caagaatatg cattattgct gctcaatagc atgccggaaa aaacagctga
     2041 gcattaccgc aataaaatcg ccatttattt gcactggtat cagaaaaaag gcatcgaggt
     2101 tccacaaacc cagcaagggg acattggcgc gaaagatatc ccctcctggc ggcggatatg
     2161 caaagtttta ctcaataacg attactggtg tcgggcatta tcatttagtc ctacgaaagc
     2221 gaagaactat cagcgttata acgaacggat aaaaggaaaa cgtcaggaat gggggatact
     2281 atgcaacaac gattaacgca ggatttaacg caatttctcg ccagcttgcc ggaagatgat
     2341 cgcatcaacg cgattaatga aatccgcatg gcgatccatc aggttagccc ctttcgtgaa
     2401 gagccagtgg attgcgttct gtgggttaaa aacagccaac ttatgcctaa tgattacaac
     2461 ccaaacaacg tggcaccacc cgaaaaaaaa ctgctgaaaa aatccattga aatagatggt
     2521 tttacccaac ccattgtggt cacgcatacg gataaaaatg ccctggaaat cgtggatggt
     2581 tttcaccgcc atgaaattgg taaaggctca tcatcattaa aattacgctt aaaaggttat
     2641 ttgccggtga cctgtctgga aggtacgcgt aatcaacgca tcgccgcgac tattcgtcat
     2701 aatcgcgctc gtggacgcca tcaaatcacc gccatgtcgg aaatcgtccg tgaactcagt
     2761 cagttgggat gggacgataa taaaattggc aaagagctgg gaatggacag cgacgaagtg
     2821 ctgcgcctga agcaaattaa cggtctgcaa gagctgtttg ccgaccgtca atattcccgc
     2881 gcctggactg tcaaataact acagctggcg cagtcgttca gctgctgcca gcaacgtcga
     2941 ttccttcttg gcaaaacaga gacgaatcag tttatgtggg aagggatcgg cgcaaaacac
     3001 cgatagcgga atcgccgcca cgccgtgctc ccgcgtcagc cactggcaaa actcaacatc
     3061 atccagggta gaaaccgcgc tgtaatccac cagcaaaaag tatgtgcctt cgcacggtaa
     3121 aatctccagc cgactttcat ttaaggcatt taccagaata tcgcgcttct ggcgataaaa
     3181 gtccggtaac gcaagataat gctcaggttc tgcacgtagc atatccgcaa ttgccagctg
     3241 tgccggggta ttcaccgaaa aggtcagata ctgatgtacc ttgcgaattt cggcgctgat
     3301 gggcgctggc gcaacacaat aacccacttt ccagccggtc atatgatagg tcttgccaaa
     3361 cgatgacacc gccaccgccc gctcacgcag ctgcggatgc gccagcacac tggcatggcc
     3421 ctgttgtgaa aagttgatgt gctcgtagac ttcatcgcta atgacaaaaa tctcgtgccc
     3481 ggcgctcgcc tgccacaaag cggcgaaatc agcctgctgc cagacagttg cactgggatt
     3541 atgcggagtg ttgaggatca ccagtctggt gcgctcgctt aacaatgcgg caaattcctg
     3601 ccagtcaacg cgaaaatgcg gtggttgcag tgccatacgc ttcactattc ccccagaaag
     3661 cgcgatggcg ggggcgtaac tgtcatagct gggatcaaaa caaatcactt catcgccatt
     3721 gcgcaccagt gcggtaatcg ccgcgtataa cgcttccgtc gcccctgccg ttacggtgat
     3781 atcgctatcg gcatctggtt gatagccata caaacgttcc gttttctgag caatcgcctc
     3841 gcgcaaggcc tgcacgccgg tcatgggcgc gtattggttt gccccctggg caacgtggta
     3901 cgccagccgc tcctgtaaat agcgcggacc atcaaaatca ggaaagcctt gcgacaggtt
     3961 aatcgcctgg tgttgctgcg ccagcgcgct catctgggtg aaaatagtgg tgccaagttg
     4021 tggaagtttg ctttgtggaa tcagagggtt atttgtcatt tttattgtac ctgacgaagt
     4081 tgcgtgttga aggcactata acacgatgct agtatttggc aatcaagacg tttagatgtc
     4141 taaataaaac aataaggaca acacaacatg cctcacaatc ctatccgcgt ggtcgtcggc
     4201 ccggctaact acttttcaca tccaggaagt ttcaatcacc tgcacgattt tttcactgat
     4261 gaacaacttt ctcgcgcggt gtggatctac ggcgtacgcg ccattgctgc ggcgcaaacc
     4321 aaacttccgc cagcgtttgg acttccaggg gcaaagcata ttttgtttcg tggtcattgc
     4381 agcgaaagcg atgtacagca actggcggct gagtccggtg acgatcgcag cgtggtgatt
     4441 ggcgtcggcg gcggcgcact actcgacacc gcgaaagccc tcgcccgccg tctcggtctg
     4501 ccatttgttg ccgctccaac gatcgccgcc acctgcgccg cctggacacc gctctccgtc
     4561 tggtataacg atgccggaca ggcgctgcat tatgagattt tcgacgacgc caattttatg
     4621 gtgctggtgg aaccggagat tatcctcaat gcgccgcaac aatatctgct ggcggggatc
     4681 ggtgatacgc tggcgaaatg gtatgaagcg gtggtgctgg ctccgcaacc agaaacgttg
     4741 ccgctaaccg tgcgactggg gatcaataat gctcaggcca ttcgcgacgt cttgttaaac
     4801 agtagcgaac aggcgcttgc cgatcagcaa aatcagcagt taacgcaatc attttgcgat
     4861 gtggtggatg ccattattgc tggtggtggg atggtcggtg gtctgggcga tcgttttacg
     4921 cgtgtagcgg cggcccatgc cgtacataac ggtctgactg tcctgccaca aaccgagaag
     4981 tttctccacg gcaccaaagt cgcctacgga attctggtgc aaagcgcctt gctgggtcag
     5041 gatgatgtgc tggcgcaatt aaccggggcg tatcagcgtt ttcatctgcc gactacgctg
     5101 gcggagctgg aagtggatat caataatcag gcggagatcg ataaagtgat tgcccacacc
     5161 ttgcgcccgg tggagtccat tcattacctg ccagtcacgc tgacaccaga tacgttgcgt
     5221 gcagcgttcg aaaaagtgga atcgtttaaa gcctgacgtg caaatttagc agcagcgcgc
     5281 gccgcctttt ccaccgtagc gcgcgtcctg ccgctcacgg aaaaactctt cgtaggtcat
     5341 cggcgtttga tcgggatggt taacccgcat atgttcgaca tagttgtcgt aatcaggcat
     5401 accaatcatc agcttcgccg cctgacctaa atattttccg gcttttgcca gtgaatcaaa
     5461 catcttggcc tccgctaacc atgttgctga aagagtgccg gatgcggcgt gaacgcctta
     5521 tccggcctac aaatgctatc aacgcgctcg gtcagccccc tcgccccatc ggggagaggg
     5581 ttagggtgag ggggaaccca cggcgccatg ccaacattcg ccaacatccc ccctcactct
     5641 gactttagtg cgcgcctttt gcctgcacca cgatctcctc gacattttcc ggcattggct
     5701 catacggcgt ttctttcgcc gttggcttcg gctctttcaa tgccgcaagt gccgtcttaa
     5761 tcgagaacag tgccagaacc accacgacca ccataaagaa gatggttaac ccggcatcca
     5821 gacggttgtt gaacaccagt tgcgccagct gtgactcagt atactgcgat ggaatattac
     5881 cgctgtcgat cattgcctgg aacttattag caatggccag gaagccgact ttcgcatccg
     5941 ggctaaacgc tttctgccag cctgcggtca gggtacaaat cagcagccag gccgttggta
     6001 ccagcgccac ccaggcgtaa cgttgacgtt tcatcttgaa caacaccacg gcacagagca
     6061 tcagcgccat ccctgccagc atctggttgg caataccaaa cagcggccac agagtgttaa
     6121 taccgcccag cggatcgacc acaccctgat gcaggaagta gccccacgcc agcacgcaca
     6181 gcgccgttgc cagcaggtta gcaggcagtg aatcggtccg tttcaggccc ggagaaacca
     6241 cgcccagcag atcctgcaac ataaagcgcg cagcacgcgt acctgcatcc accgccgtca
     6301 gaataaacag agcttcaaac agaatggcga agtgatacca gaacgccaca tccatcatgc
     6361 cgcccagcgc gccatgcaga atgtaggcca tccccaccgc caacgtcggc gcaccgcccg
     6421 cacgggaaat gatcgactgt tctcccactt cgctggcaat ctggttgagc gtatccggag
     6481 taatcgcaaa gccccagcta ctcaccactt gcgcggcaga agcgaccaca tccgccgtcc
     6541 ctgccggagc cagcaccgcc atcgggctgt tcatggcgaa gtacacgccc ggatcgatga
     6601 tacaggcgga aaccagtgcc ataatcgcca cgaaggattc cattaacatt ccaccgtagc
     6661 cgataaaaca agcctgccct tcgttcgcca gcatcttcgg cgtggtgccg gaagagatca
     6721 gcgcatggaa gccagacacc gcgccacagg cgatggtgat aaacaggaac gggaacaggt
     6781 taccggtcca taccgggcca gtgccgtcaa caaatttggt caacgcaggc atggtcagcg
     6841 tcgggcgcat aatcaaaatg cctaccgcca gaccaacgat cgtcccgatt ttcaggaagg
     6901 tagagaggta gtcacgcgga gccagcagca accataccgg cagcaccgcc gccacaaaac
     6961 cgtaacccac cagcatccag gtcagctgca cgccggtaaa gtcaaagtac ggtgcccagg
     7021 tcggactttc tgccacccag ccgccagaaa taatggcgaa aatcaggaat accagaccga
     7081 tgaccgacac ttcaccaata cgccccggac gcagatagcg caggtagatc cccatgaaca
     7141 gcgccagcgg aatggtgaac gcaacggtat acgttcccca cgggctatga gtcagggctt
     7201 tcaccacgat catcgccagt actgcaagga taatgaccat gatcataaag caggccacca
     7261 gcgcgatcac cccggcggtt ggccccatct cttctttgac cagctcaccc agcgaacgac
     7321 cgtcacggcg cgtagaaaca aacagcacca tgaaatcctg caccgcaccg gcgagaacca
     7381 ccccggcgag tagccagatc atccctggca ggtaacccat ttgcgccgcc agtaccggcc
     7441 caaccaacgg acctgctccg gcaatggccg caaaatggtg accgaacagc actttcttgt
     7501 ccgtcggcac atagtccagc ccgtcgttat ggcgcaccgc tggcgtcata cgcgtcgggt
     7561 caaccgccag cacatttttg gcgatataca gaccataaaa acggtaagcg atcagataga
     7621 tacagaccga cgccaccaca atccacagcg cgttgatctg ttccccacga tttaaagcaa
     7681 tatatcccag agcaaatgct cccattacag agagcactgt ccagacgagg tatttccctg
     7741 atttgttcat agttgttatc cgtgtgcgtg tccatagaga tgttacattt tgtttctata
     7801 acatctttgt acgatttaac aacacagcaa ccatgtaaat cggagttgaa accaatattt
     7861 aacttaacaa tgttaactcg atcactccgt taaccgagtt ttcctgcaat ctcatataat
     7921 tctgtcgggt tcatcccaaa actgccgtac ccagccgaca agtgcagcaa cgccgcccct
     7981 gttcatcgaa aacgacgatt tcccagctct gattttgccg cccaagatgc agcggctggc
     8041 agacgccgcg taccttgcct tcagacaccg ggcgatgatg tgtggcgtta agttccgtgc
     8101 ccaccacaca ctgcccgtcg cgggtcatca taaatccggc catcgatccc agcgtttcag
     8161 ccagcgccgc cgacgcgccg ccatgcagca ggccaaacgg ctgatgagta cggatatcaa
     8221 ccggcatttc ggcttccagc acatcatcgc ccagacgggt atacacaatt cccagatgcg
     8281 ccaccattgt gttatcgctg gtggcgttca gttcgtcgag cgttaaatgg cgtttccaga
     8341 tcatgcttat gcccccagcg ttgagccgcc atcgaccaca atatcctgta gggtgatatg
     8401 gctggcgagg tcagaggcga ggaacaaaat cgtgttggcg atctcttgtg gacgggcgat
     8461 tttccccagc ggaatgccga gtttaaactg ctcgccaaag ccgcgaatac gctgttcttc
     8521 ggcgtcatcg ctcacccaca gcgtgcgttg catatcggta tcggtggaac ccggcgaaac
     8581 cacattacag cgcacgccgc tacccgccag ttccagcccg acgctcaacg ccaggctttt
     8641 cagcgccgct ttcgatgcgc cataagcact cataccaata cgcggcgtgt gtgcggcgtc
     8701 ggacgccaca gtgacaatcg ccccgccccg ctgacggcga aactggttca tggtttgctg
     8761 gaacaggtta aacgcaccgc cgacattaac cgcaaaagtc tgctgccagt cctccttact
     8821 gagctgatcg gtcgcgccca tgcgcaaaat tcccgccgca ttgaccagca cgtccagtcg
     8881 ctccgtttca gctaacagtc gctgacatac ctgcgccacc tgcgcagcat cggcaacatc
     8941 cattacttcg gtcgcaaagg gatattgctc ctgagcgaac gcttgatcaa aacctgtaac
     9001 tttcgctccc gcttcaacaa acgccagcgc cgtggcgtag ccgatacctt tccccgcgcc
     9061 ggttacccag acatttttac cgctgaaatc cattatttca cctcgcggga gagtagcttc
     9121 caccaggcgt cgatggtcgg gtttttcgcc aacatgacaa agtcgatatc gccatgcact
     9181 ttgcgccagc gcgccgccag cgccatcatg cgcaccgaat ccagaccgta gtcgatcagg
     9241 ttgtcgtcat cgaacggttc atcggactcg tccagcaacg gcaggatcac ctcacgcagc
     9301 gccgctttgc tggcggggat aggtgctggc agtaattctt cggtcatcac cacccggcca
     9361 gaacgtccgg ccacatattt cagcgacatc aaatgctcgt cacggctgaa atcggccagc
     9421 gcatccgcca ccataaacgg tttaatatcg cgcataaatg cgtcggttgc ggtggtcata
     9481 cagccaatgt gggcatatac cccggtaata atcagctggt tacgtccact ctctttgagc
     9541 atttgctcca gcggagaacg atgaaacgcg ctgtagcgcc acttcaccag caccgtgtcg
     9601 tcggcatctg gcgtcagtcg atccaccacc ttttgctgtt ccggcgaacg ggtcaggccc
     9661 ggcccccaca tatcattcaa cagcgcccga tcttcatcgc tctgctcttt cggctgggcg
     9721 gtgtaataaa ccgggatatt gtgctgtttg cagtagtcgc gcagcgcagc aatattcgcg
     9781 atcacctgct ccatcatcgg gcagttctcg ccccagaagc tgacaaaata gtcctgcata
     9841 tcatggatta acaacgcggc acgttgcggt tcaaaggccc agtcaacttt attctgcgga
     9901 atatcgtgag actccggcag tgcgtaagcc tgtaattttg gaatagccat cgtgttctct
     9961 ccttcaggct gatgcgcgtg acgccagcca ctgacgtaat tgttttttat cgactttccc
    10021 gaccgccgtc agcggaagtg aatccacaca ctccacgcga tccggtaatt taaattcggc
    10081 aataccctgt tcacgcagga aacgacgcac ctgcaccgcg cgaagcggct cttttaccac
    10141 cagataagca cagctttttt cgcccatcag ctcatcttcc atactgacca gtgcggcgta
    10201 gatcaccgcc ggatggcgca gtaacaggtt ttcgatctct tcggcagcga tcttctcgcc
    10261 gccacggttg atctgatctt tctcgcgccc ctgcacggtg atgtaaccct ctggatcaat
    10321 agagatcaga tcgccggaac agtaaaaacc gttggcatca aaggcgctgg cattgtgctg
    10381 cgggcttttg taatagccac ggaaggtata cggcccgcgc gtcatcagtc gtccgacttc
    10441 cccttgcggc agtggatttc cttcggcatc ggcaacccat acttcgtcat ccggacacat
    10501 tgggtaaccc tgggtatgga taattttctc cgcgctatca tcaagacggg tatagttcac
    10561 cagcccttcc gccataccaa acacctgctg caactggcaa ccaatctcag cgggaatacg
    10621 cgccgcaagc gtggcagaaa gacgtgcgcc accgacctgt aacagtttca gcgaggcaag
    10681 ctgcgcccgg ctttcgcctt cggtcagcgc ctgcaaccac aggctgactg ccggcggcac
    10741 cagcgcggta acgttaatct gatgtttttc aatcaatggg aagcaaagcg tggcgctggg
    10801 atcggccgcc agaacaaccg ttcctccggc aagaaagacg cccagcgatc ccggcgaact
    10861 catggcgtag ttatgagccg ccgggatcgc gcacaggtag cgtgtctgtt gtgtgaactg
    10921 acaaatctcg acgctacgac gcacgctgta gtagtagtcg ttatgagtgc gcgggatcag
    10981 cttcggtgta ccggtggtgc cgccggaaag ctggaaatag accacttcat cagcaggtga
    11041 cggcgtagca gtaaaaccgt ctgccggatg gttaatcgca tcctgcaagt tatgctcacc
    11101 gctgtcgttg agtagctgca ccacgcgaat ggaagaatgc tctgcaacaa atgtgttgag
    11161 gaaatcatcc ccgctaaaca gcgcatgttg gcgatcggca atcagcaatg cgggttcaat
    11221 ctggctggca taggcgttca gttcactacg ctgatggctg aacaacgcca gcaccggcgc
    11281 aacgcccagt ttcagcagcg cgaaaaaggt aatgtaaagt tcagcgacgt tacccagttg
    11341 taccagcgcg gtttcaccag gtttaatgcc ctgacggcgt aaactacacg cgaggttatc
    11401 cgccgcctga ttcagctccc ggtaactcaa ctgtcgctcg ccgtcgataa ccgcgatgct
    11461 gtcactcgca gcgtggcgag tcagaatgtc agtcagtggc aaatcctgcc agtagccttt
    11521 ttcccgatag cgacgggcaa actcttccgg ccagcgggtg aatggaatgc tcatcctcgc
    11581 tccttaatgc aatccaaaaa cgttcaacat ggtagaaagt ttgacgcctg tttcgcgcca
    11641 ctcacccaac ggtgacgacg caggcacaat ccccgctccg gcaaacagac gcacctgatt
    11701 ttcccgcagc ttcgcgcagc ggatggtcac cacccattcg ccgttacctt cgctgtcaca
    11761 ccaacccaca atgccgccaa acagttcgcg gtcgaacggc tccagttcag caataacctg
    11821 ggtcgcggcc tgatgcggga aaccgctcag cgcgggggtc ggatgcagca gacaggccag
    11881 agtcagtgcg ttttcttgcg aattcgcttt accttcaaag ggagttgcga gatgccacag
    11941 cgtcggcgtg gtaatcaatt gtggagagga aggaacgtgt aactcactac tgcgttcgcg
    12001 cagtacctct ttcatcgcct gagtcaccag ttcatgttca tggcgatctt tttctgacgc
    12061 cagcagacga ttacccgctt cgcgatcgag cacttcatcc ggctgacgac gtgcggaacc
    12121 ggctaacgga atggagctaa aacgctcacc gtctttacgt agcagcagtt ccgggctggc
    12181 ccccagcaga acgccaccat ctgccagcgg gacatggaag ttgtaactaa ccgggttttg
    12241 cgcaatcaac cgttccagca atacgccact atcaatggcg gcgtcagtgg tgatatcaat
    12301 caaccgtgac aacaccactt tgtcgacctg cggcgtggcg gtaagtgcgg cggcgcgggc
    12361 aaccatctgt tcaaacgtgg tttgctccgg aattgcctgg cgttccacca cgttcagcga
    12421 ctggctgcgg gtgaaacggc gtgcggaagc ttgtttttcc tgacgggaga acgactgcca
    12481 ggattcagga atatacagcg acgaaggctg acgtggatcg aaaggaatcg ccccgaccat
    12541 caccggattt ttgatgccct gcgctttggc atcggcaaac agcgcggcga gtttttgctg
    12601 gaagggactg tcgggcgaat ccccgttcac agccggttca tcgaagcggg cgaaacatcc
    12661 tgacgtcgta aaactgcggt acggcgacat aaagaaaaag cgattgggcg caagtgttgc
    12721 catggtctgc tgtacttcct cagccagtga cgtatccata tcatcctcca caaaatgata
    12781 aaggctttaa taatgattat catttatatt ttcggtcgct aacctaaaag caaacgcgca
    12841 ctatgtcaac tcttgaggta acgcaatctg caactcgggg ttgcatctgg ttgtggttat
    12901 aatgaaaatg agaagcatta acttcattaa taacaggagg ctgatttgtg agactcgccc
    12961 cgctctaccg caatgccctt ctattaacag gacttttgtt ttcaggaata gccgcagttc
    13021 aggccgccga ctggccgcgt cagattactg acagccgtgg cactcatacc ctggaaagcc
    13081 agccgcagcg tattgtttcc accagcgtca ccctgaccgg ctcactgctg gcgattgatg
    13141 ctccggtgat cgccagcggc gcgaccacgc cgaataaccg cgtcgcggat gaccagggct
    13201 ttttacgcca gtggagcaag gtggcgaaag aacgcaaact gcaacggctc tatatcggcg
    13261 aaccgagcac cgaagccgtt gccgcgcaaa tgccggatct gattttaatt agcgcaaccg
    13321 gcggggattc ggcgctggcg ctgtatgatc agctttccac catcgccccg acattaatca
    13381 tcaattacga cgacaaaagc tggcaggcgc tgttaacgca acttggcgaa attaccgggc
    13441 atgagaaaca agcggcagag cggattgcgc agtttgataa gcaactggcg gcggcgaaag
    13501 agcaaatcaa attaccgccg cagccggtca ctgccattgt ctatactgcc gctgcacaca
    13561 gtgccaatct ctggacgccg gaatcagcac aagggcagat gctggaacaa ctcggcttta
    13621 cgctggcgaa gttacccgca gggttaaatg ccagccaaag ccagggtaaa cgccatgaca
    13681 tcattcagct tggtggggaa aatctggctg cagggttaaa tggcgagtca ctattcctgt
    13741 tcgccggtga tcagaaagat gccgatgcta tttatgctaa tccgctgctc gcgcacctgc
    13801 ctgcagtaca aaacaagcag gtttatgcgc tgggaaccga gacgttccgt ctggattact
    13861 acagcgccat gcaagtgctg gaacgactta aggcgctgtt ttaagcatta accgtcagac
    13921 gctgtcacct gcggcggcgt ctggcgaaaa cgtcgcaact ccaccagcac cagcaataac
    13981 aacacgccga taatcaataa accaaaaccg ctcgcgcttg cggaagcaac cggtgtcatc
    14041 atcgctccca aaccacccag cagcgccgcg cctatggcat cgcccgtcac gttttgtgcc
    14101 gtccacaaac cgttaatccg ccctaacatc gcttccgggg tttgcgtttg cagcattgtg
    14161 tattgcagca acgagctgac cgcactcaac cagccgaaca gcgccagaca aaccacgcct
    14221 aaaatccaca tcggcatcag gccaaacaga ccaatggcga ggaacgatcc cagcgtggag
    14281 agcagcatca ataaccctgg tcgcgcgcta tgcgccagct tcccgctggt taacgcgcca
    14341 atagccgcgc cgagcgggat cgccgcatag agaaaaccaa tctgtgccgc cgacatctgc
    14401 cagttgtcag ccagcgccgg atacagtacc cgcaccgcac tcgccatcgt caataaacca
    14461 cccagcagcg caatccctcc caccagcggg ctggcgagta gaaaacgaaa tcctgccagt
    14521 aatgatttca acggatgctc acgcggctgc ggtggcggtg gcagtgccgg aaggcttaac
    14581 aacggtagca aggtaataaa cgtgcccgcc gccgccagcc cgtagttcca ggccacaccg
    14641 ccagtcgcca gcagtaaacc gccaatcatg ggcgaaatca ccgaccccag acgcacggtc
    14701 aacatggtga tcgccccggc ctgcattaag ttttcacgcc ctaccagtgc tggtgtcgcc
    14761 gccaatagcg ccgtaacgcc aagcgatgcg aaaaaaccat cccataaacc aagtaaatag
    14821 attgccagca atgacggctc cggcagcagc gcgttcaggc acagtccaat gaagccaatg
    14881 ccacaggtgc cgcgcgccag caaaatcact tttttgcgct catagcgatc cgccagcaca
    14941 ccgccgacca tcaggccaac aaacatcgcg ccgccggtca gcgtcaccga aagccccacc
    15001 tgccaggtgg aatgtgtcat catctggatc tgcaccggca ccgcgacgcc gagcaaaccc
    15061 agagacacaa ttgagatgaa acgagcgagg aatactgcgc gaaacgccgg gtgcgttttc
    15121 aacaggctga ggttaagcag ccaggattgt ttattcatta caatgccttg ccatcaaata
    15181 ttttaaatac ctattcttag gctgcacatg ctaacatatc caaataagat cgataacgat
    15241 aattaatttc attatcatgg aagttcgtat gtctggttct gttgccgtga cacgcgccat
    15301 tgccgtgccc ggattgctgt tattactgat tatcgcgacg gcattaagcc tgctcattgg
    15361 ggcaaaatca ctccccgctt ccgtagtgct ggaggccttc tccggcacct gccagagcgc
    15421 cgactgcacc atcgtgctcg acgcgcggct gccgcgtacc cttgccggtt tactggcagg
    15481 cggcgcgctt ggccttgccg gggcgttaat gcaaaccctc acccgaaacc cacttgccga
    15541 ccccggcttg cttggcgtga acgccggagc cagctttgcc attgtgctgg gtgcggcgct
    15601 gtttggttac tcttccgcac aggaacaact ggcgatggcc ttcgccgggg cgctggtggc
    15661 ctcgttgatt gttgccttta ccggcagcca gggcggtggg cagttaagtc cggtgcgttt
    15721 aaccctggcg ggcgtggcgc tggcggcggt gctggaaggg ctgaccagcg gcatcgccct
    15781 gcttaatcct gacgtctacg atcaactgcg tttctggcaa gccggttcgc tggatattcg
    15841 caatctacat accttaaaag tggtgctgat cccagtgctg atcgccggag caactgcgct
    15901 attactgagt cgcgcgctga acagtttaag ccttggcagc gacaccgcga cggcgctggg
    15961 cagtcgcgtg gcgcgcacac agttgattgg tctgctggcg attaccgtgc tttgtggtag
    16021 tgcgacggcg gtagttggcc ctattgcctt tattggcctg atgatgccgc atatggcgcg
    16081 ttggctggtg ggtgccgatc atcgctggtc gctgcccgtc acgctacttg ctacccctgc
    16141 cctgctgctg tttgccgata tcatcgggcg ggtgattgtg cccggtgaac tgcgcgtttc
    16201 tgtggtcagt gcgtttattg gtgcaccggt gctgatcttc ctcgtgcgac gtaaaacgcg
    16261 aggtggtgca tgatttacgt ctctcgccga ttactcatca cctgtttgct gctgatttcc
    16321 gcctgtgtgg ttgcaggtat ctggggatta cgcagcggtg ccgtcacgct ggaaatctcg
    16381 caggtattcg ccgcgctgat gggcgatgcg ccgcgcagta tgacgatggt ggtcaccgaa
    16441 tggcgtttac cacgcgtgct gatggcgctg ttgattggcg cggcactggg cgtcagcggt
    16501 gcgatttttc agtcgctgat gcgtaaccca ctcggcagcc ctgacgtaat gggctttaac
    16561 accggggcgt ggagcggcgt gttggtggcg atggtgctgt ttggtcagga tctgacggct
    16621 atcgcgctgg cagcaatggt gggcggcatt atcacttcgc tgttggtctg gctgctcgcc
    16681 tggcgtaacg gtatcgacac ctttcggttg attattatcg gtatcggcgt tcgcgccatg
    16741 ctggtggcct ttaatacctg gctgttgctg aaagcgtctt tagaaacggc gctaacagca
    16801 ggtttgtgga atgccggatc gctcaacggc ctgacgtggg caaaaacctc gccttccgca
    16861 cccatcatta tattgatgct aattgccgcc gccttactgg tacgccggat gcgcttgctg
    16921 gaaatgggcg atgataccgc gtgtgcgctg ggcgtcagcg tcgaacgttc gcgtctgtta
    16981 atgatgctgg ttgcagtggt gcttaccgct gcggcaacag cgcttgccgg gccgatttcc
    17041 tttattgctt tagtcgcgcc gcacattgcc cgacgcatta gcggcaccgc tcgctggggg
    17101 ctaacccagg cggcgctatg cggggcgctg ttactgctgg cggccgatct ctgcgcccaa
    17161 caactgttta tgccgtatca acttccggtt ggcgtcgtta ccgtcagcct cggcggtatt
    17221 taccttatcg ttttgttaat tcaggagtct cgcaaaaaat gaccgaatca gtagcccgtt
    17281 tgcgcggcga acagttaacc ctgggatatg gcaaatatac cgttgcggaa aatctgactg
    17341 tagaaatacc tgatggtcac ttcacggcaa ttatcgggcc aaatggctgc ggtaaatcca
    17401 cgttgctgcg taccttaagc cgcctgatga cgcctgctca tgggcatgtc tggctggatg
    17461 gcgagcacat tcaacattac gccagtaaag aagttgcccg gcggattggt ctgttggcgc
    17521 aaaatgccac cacgctggga gatatcaccg tgcaggagct ggtggcgcgt gggcgttatc
    17581 cacatcaacc gctgtttacc cgttggcgca aagaagatga agaggcggta acgaaagcga
    17641 tgcaggccac gggaataact catcttgcag atcaaagcgt ggataccctt tccggtgggc
    17701 aacgccagcg agcgtggatc gcgatggtgc tggcccagga aacggcaatt atgctgctcg
    17761 acgaacccac gacctggctg gatatcagtc atcagattga tttgctggaa ctgttaagcg
    17821 aactgaaccg cgagaaaggc tataccctgg cggcggtgct gcacgatctt aatcaggcct
    17881 gtcgttacgc cagccatttg attgcattgc gagaagggaa aattgttgct cagggagcgc
    17941 cgaaggagat tgtcactgct gaactgattg agcgcattta tggtctccgc tgcatgatca
    18001 ttgacgatcc ggtagccgga acgccgctcg tggtgccgct cggacgaacg gcaccttcaa
    18061 ccgcaaatag ttaaactaag tggtctgcca tcatggcatc ctgttttctg gatgccatcg
    18121 catagcgcaa taacacgcta ccacaagcca ccatcccgcc gatcagcgcg gaaaggatca
    18181 caataatcgc cttacccgga ccgtcttttt tcactggcaa tgacgggctt aactgatatt
    18241 taaacggcgt aaaattcaca tcgttgatat ttgcttttgt taactgctca acaagatact
    18301 gacgattacg taattcaccg ttcagttccg caacgtcagt gaccgctttt tcaatttcca
    18361 gtttgcgttc aataccgtct gcgccgagag aaatagagaa atcggggtca tctttaacgg
    18421 cctgaccatt actgtaaacg ggctttttaa ttcctgccgc gttggcaatg tcgagtgaat
    18481 aattgaggcg ctgaatgttt gcatcaagtt gatttttcat tttaatgcgg tcctgagcca
    18541 gtttttcttt ttcaaactgg gttttgatct ccagtttatt acggacgttt tctatcgact
    18601 ctttcaccac caacgcagag atataatcga tatacccgct caaaacggtc tgcgcctctt
    18661 cactggttgg cgcggtaaaa cttagcgtcc aggaggtata cagtgacggt tcatcttttt
    18721 tcttactggc attgtcatca accgctttca ttttttcgct caatgcgaca attgcgcgat
    18781 gcaaatccag ttcgtcgatt ttcgcctctt ttaattggtc catcacataa ggtgatgaac
    18841 gcaggtactc ttccagcaag ctaaccgact gaaacttctt gataaacagg ttaaatgctt
    18901 ctgtacgatc aattttgata tccagatcca gcacacgaag cttagtgaat gttttctcca
    18961 actcttgcca ctgaacaggt tctggaggcg tgacaaccgc cgcgctggtc catttttgcg
    19021 gcaggatgaa agagatcagc aagcctgcac aggcaaacgc aaaaacgacc gccatgaccg
    19081 tttttttagc ccgccataaa actgagatta gattaagtaa atcaatttca ttattactgg
    19141 gcgacgccag aggataatcg ggaaaatgag cgtcacttcc ctgtttaata ttcagtgatg
    19201 acatggggta taacctgaaa atgagtccaa tagggggaaa tctccacggc gctcacttta
    19261 ccagttggtg agaattatcc gaagctgaag tttgtaaatt cattccactg aatgcaacat
    19321 tcactctatt taagaaaaat aaccagcaaa c
//
LOCUS       DAZFAB010000085        19240 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZFAB010000085 DAZFAB010000000
VERSION     DAZFAB010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.085"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(281..1930)
                     /gene="pgi"
                     /locus_tag="ACHJWA_003445"
     CDS             complement(281..1930)
                     /gene="pgi"
                     /locus_tag="ACHJWA_003445"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFO4309975.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     regulatory      2167..2363
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2455..3804
                     /gene="lysC"
                     /locus_tag="ACHJWA_003446"
     CDS             2455..3804
                     /gene="lysC"
                     /locus_tag="ACHJWA_003446"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFO4309976.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            4057..4329
                     /locus_tag="ACHJWA_003447"
     CDS             4057..4329
                     /locus_tag="ACHJWA_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFO4309977.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(4330..5202)
                     /gene="rluF"
                     /locus_tag="ACHJWA_003448"
     CDS             complement(4330..5202)
                     /gene="rluF"
                     /locus_tag="ACHJWA_003448"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFO4309978.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            5550..6497
                     /locus_tag="ACHJWA_003449"
     CDS             5550..6497
                     /locus_tag="ACHJWA_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313031.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFO4309979.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTNISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGEMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            6567..7373
                     /locus_tag="ACHJWA_003450"
     CDS             6567..7373
                     /locus_tag="ACHJWA_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO4309980.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEIADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            7383..7790
                     /locus_tag="ACHJWA_003451"
     CDS             7383..7790
                     /locus_tag="ACHJWA_003451"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFO4309981.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQNEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            7790..8284
                     /locus_tag="ACHJWA_003452"
     CDS             7790..8284
                     /locus_tag="ACHJWA_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFO4309982.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIYKINEQ
                     LIAN"
     gene            8350..9147
                     /locus_tag="ACHJWA_003453"
     CDS             8350..9147
                     /locus_tag="ACHJWA_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFO4309983.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLLAPASTALDQLDD"
     gene            9158..9982
                     /locus_tag="ACHJWA_003454"
     CDS             9158..9982
                     /locus_tag="ACHJWA_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFO4309984.1"
                     /translation="MEQRKITLGDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPVKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VTMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            10034..11263
                     /gene="sorE"
                     /locus_tag="ACHJWA_003455"
     CDS             10034..11263
                     /gene="sorE"
                     /locus_tag="ACHJWA_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HFO4309985.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRMLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLTHAEAISHD"
     gene            11646..12335
                     /gene="pepE"
                     /locus_tag="ACHJWA_003456"
     CDS             11646..12335
                     /gene="pepE"
                     /locus_tag="ACHJWA_003456"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFO4309986.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(12426..14057)
                     /locus_tag="ACHJWA_003457"
     CDS             complement(12426..14057)
                     /locus_tag="ACHJWA_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFO4309987.1"
                     /translation="MLTLLNLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLAMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMSSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(14277..17960)
                     /gene="metH"
                     /locus_tag="ACHJWA_003458"
     CDS             complement(14277..17960)
                     /gene="metH"
                     /locus_tag="ACHJWA_003458"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFO4309988.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWEVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDIANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            18160..18984
                     /gene="iclR"
                     /locus_tag="ACHJWA_003459"
     CDS             18160..18984
                     /gene="iclR"
                     /locus_tag="ACHJWA_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFO4309989.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         4547 a         4997 c         5043 g         4653 t
ORIGIN      
        1 acgattaaaa atctatttta ggattaacct cacggtatga tttccgttaa atcacagaca
       61 aggggattta tctgataaaa caatgccgac ttatagtcgg cattgttttt aaggttattt
      121 aattaatcgt gctatgcatt aattgccgaa tacgatgtac atatcggcat cgacctgtag
      181 gcctgataag acgcgtaaag cgtcgcatca ggcaccagtt gccggatgcg gcgtaaacgc
      241 cttatccggc ctacatagcg gcatcaggca tgaacgatga ttaaccgcgc cacgctttat
      301 agcggttaat cagaccattg gtcgagctat cgtggctgct gatttcttta tcatctttca
      361 gctctggcag aatacggttc gccagctgtt tacccagttc cacgccccac tggtcgaagg
      421 tgaagatgtt caggatcacg ccctgagtaa agattttgtg ctcatacagc gcaatcaacg
      481 cacccaggct gaacggagtg atttcgcgca acaggataga gttggtcggg cggttacctt
      541 cgaacacttt gaacggcacc acgtagtcaa gcgttgccgg atctttaccc tgatcgcgat
      601 attcctgctc aaccacttcg cgggatttac caaacgccag cgcttcggtc tgggcgaaga
      661 agttagacag cagtttctgg tggtgatcag agagcgggtt atgggtgata gccggagcga
      721 tgaaatcgca cggtaccatt ttggttccct ggtggatcag ctggtagaac gcgtgctgac
      781 cgttagtgcc tggttcaccc cagataatcg ggccagtctg gtaatccaca acgttaccgt
      841 tacggtcaac atacttaccg ttggactcca tattgccctg ctggaagtac gccgcgaaac
      901 ggtgcatata ctggtcatac ggcagaatcg cttcagtttc cgcaccaaag aaattgttgt
      961 accagatgcc aatcagcgcc aacagtacag gcaggttttt ctcggcaggc gtggtggaga
     1021 aatgcttgtc catcgcgtgt gcgccagaaa gcagttcaac gaagttatca aagccgatgg
     1081 agagaacaat cgacaggcca atcgctgacc acaaagagta acgaccgccg acccagtccc
     1141 agaactcgaa catgttggca gtatcaatac caaactcgcc aacggctttg gcattggttg
     1201 agagcgccgc aaagtgtttc gcaacgtgtt tttcatcacc tgccgctttc aggaaccagt
     1261 cacgcgcgct atgggcgttg gtcatggttt cctgagtggt gaaggtttta gatgctacca
     1321 ggaacagcgt ggtttccggg tttacttttt tcagcacttc agcgatgtga gtcccatcga
     1381 cgttagaaac aaagtgcatg ttcaggtgat ttttgtacgg acgcagtgct tcggtcacca
     1441 tgtacgggcc gaggtcagaa ccgccgatcc cgatgttcac tacgtcagtg attgctttgc
     1501 cggtataacc cttccactca ccggagataa tcgcttccga gaaggttttc atcttctcca
     1561 gcaccgcgtt gacttctggc ataacatctt tgccatcaac cagaatcggg gtattgctac
     1621 gattacgcag cgctacgtgc agcacggcgc ggttttcagt gcggttgatc ttctcgccag
     1681 agaacatcga cttaatcgcg cccgccagat cgcactcttt cgccagatct tgtaatttcg
     1741 ccagcgtctc ttcagtgatg cggtttttgg agtaatccac cagcatctga tcgtcgaagg
     1801 ttgcggagaa cttagaaaaa cgatcgccgt ctttagcaaa aagatcggcg atcgtaacgt
     1861 ctttcatttc atcgaagtgt ttctgtagtg cctgccaggc agcggtctgc gttggattga
     1921 tgtttttcat tagcaatact cttctgattt tgagaattgt gactttggaa gattgtagcg
     1981 ccagtcacag aaaaatgtga tggttttagt gccgttagcg taatgttgag tgtaaaccct
     2041 tagcgcagtg aagcatttat tagctgaact actgaccgcc aggagtggat gaaaaatccg
     2101 catgaccccg tcgttgacaa ccgccccgct caccctttat ttataaatgt actacctgcg
     2161 ctagcgcagg ccagaagagg cgcgttgccc aagtaacggt gttggaggag ccagtcctgt
     2221 gataacacct gagggggtgc atcgccgagg tgattgaacg gctggccacg ttcatcatcg
     2281 gctacagggg ctgaatcccc tgggttgtca ccagaagcgt tcgcagtcgg gcgtttcgca
     2341 agtggtggag cacttctggg tgaaaatagt agcgaagtat cgctctgcgc ccacccgtct
     2401 tccgctcttc ccttgtgcca aggctgaaaa tggatcccct gacacgaggt agttatgtct
     2461 gaaattgttg tctccaaatt tggcggtacc agcgtagctg attttgacgc catgaaccgc
     2521 agcgctgata ttgtgctttc tgatgccaac gtgcgtttag ttgtcctctc ggcttctgct
     2581 ggtatcacta atctgctggt cgctttagcg gaaggactgg aacctggcga gcgattcgaa
     2641 aaactcgacg ctattcgcaa catccagttt gccattctgg aacgtctgcg ttacccgaac
     2701 gttatccgtg aagagattga acgtctgctg gagaacatta ctgttctggc agaagcggcg
     2761 gcgctggcaa cgtctccggc gctgacagat gaactggtca gccacggcga gctgatgtcg
     2821 accctgctgt ttgtcgagat cctgcgcgaa cgcgatgttc aggcacagtg gtttgatgta
     2881 cgtaaagtga tgcgtaccaa cgaccgattt ggtcgtgcag agccagatgt agccgcgctg
     2941 gcggaactgg ccgcgctgca gctgctccca cgtctcaatg aaggcttagt gatcacccag
     3001 ggatttatcg gtagcgaaaa taaaggtcgt acaacgacgc ttggccgtgg aggcagcgat
     3061 tatacggcag ccttgctggc ggaggcttta cacgcatctc gtgttgatat ctggaccgac
     3121 gtcccgggca tctacaccac cgatccacgc gtagtttccg cagcaaaacg cattgatgaa
     3181 atcgcgtttg ccgaagcggc agagatggca acttttggtg caaaagtact gcatccggca
     3241 acgttgctac ccgcagtacg cagcgatatc ccagtctttg tcggctccag caaagaccca
     3301 cgcgcaggtg gtacgctggt gtgcaataaa actgaaaatc cgccgctgtt ccgcgcgctg
     3361 gcgcttcgtc gcaatcagac tctgctcact ttgcacagcc tgaatatgct gcattctcgc
     3421 ggtttcctcg cggaagtttt cggcatcctc gcgcggcata atatttcggt agacttaatc
     3481 accacgtcag aagtgagcgt ggcattaacc cttgatacca ccggttcaac ctccactggc
     3541 gatacgttgc tgacgcaatc tctgctgatg gagctttccg cactgtgccg ggtggaggtg
     3601 gaagaaggtc tggcgctggt cgcgttgatt ggcaatgacc tgtcaaaagc ctgcggcgtt
     3661 ggcaaagagg tattcggcgt actggaaccg ttcaacattc gcatgatttg ttacggcgca
     3721 tccagccata acctgtgctt cctggtgccc ggcgaagatg ccgagcaggt ggtgcaaaaa
     3781 ctgcatagta atttgtttga gtaaatactg tatggcctgg aagctatatt tcgggccgta
     3841 ttgattttct tgtcactatg ctcatcaata aacgagcctg tactctgtta accagcgtct
     3901 ttatcggaga ataattgcct ttaatttttt tatctgcatc tctaattaat tatcgaaaga
     3961 gataaatagt taagagaagg caaaatgaat attatcagtt ctgctcgcaa acgaattccc
     4021 gtacactgaa ctctctggct cactgagggc accatcatgg cactcccccg tattacccaa
     4081 aaagagatga ctgaacgcga gcagcgtgaa cttaaaacac tgctggatcg ggcgcggatt
     4141 gcgcatggtc gcgtactgac caactccgaa accaacagca ttaagaaaga gtacatcgat
     4201 aaattaatgg tcgagcgtga ggctgaagcg aaaaaagccc gccagttaaa gaaaaagcag
     4261 gcttataaac cggatcctga agcgtcattt agctggtcag caaatacgtc gacgcgcggc
     4321 aggcgctgat tagcgcccct tcttttttcg ccccggcgag gtaaaacgct taccgttgga
     4381 cgccgggcga ccgccttttt ccgccgtttt ttccatctta acgactggac gtttgatgcc
     4441 cgctgttttc ggtttggcct tcgctttagg ttttgcctcg gaagaggaat tttcgatgag
     4501 cttaaagagg tcgattaact catcgtcggt taaatcgcgc cactccccca gcggaatgcc
     4561 gcttaagctg acgttcataa tgcgcgtacg ttccagcttt ttcacttcat agccgaaatg
     4621 ctcgcacatg cgccggatct gacggttcag cccctgcacc agggtaatgc ggaagacaaa
     4681 cggcgcttct tttttgactt tgcacttttt agtcaccgtc ccgaggatcg gcacccccgc
     4741 ccccatacca cgaataaatt catcggtaat cggtttatcg accgtcacca gatactcttt
     4801 ctcatgatcg ttacccgcac ggaggatctt attcaccaga tcgccgtgat tggtgaggaa
     4861 aatcagcccc tgggagtctt tatccaggcg gccaatcggg aacacgcgtt tgctgtggtt
     4921 aacgaaatcg acaatgttat cgcgctcgcc atcttcggtg gtgctgacaa tgcctacggg
     4981 cttgttcagg gcgataagta ccaaatcttc ggcttcccga ggctcaatca actgaccgtt
     5041 tactttcaca acgtcgccgg gtttcacctg atcgccaatg gtggctcgct tgccattaag
     5101 gaacacattg ccttgctcga tatagcgatc cgcttcgcgg cgtgagcaaa ttccgctttc
     5161 gctgatgtat ttatttaaac ggactgatga gtcgggcagc ataaattctc ctgtaaaacg
     5221 cggaatatac cgtacctctt agcggggaaa aacagggtta tgcggcaacg tcgaaattaa
     5281 atgtgatctg ccacgccttt tatcacaaaa gtgtctccgg aagtacaatg caaatttgcg
     5341 tggcaaaata aggataatca acatattacg aatcgctgca cacctgtgct gataactact
     5401 gcgcagagaa acaaatgtgc aagagaaagc gctatttctc cccatgcaaa gagtgaaaaa
     5461 tcgctttgaa aggccatcat gatggggtta tatcagatca agcggtgaga aagcgtattt
     5521 gcacaaatgt gcagaaccag gaggcggcaa tggaaaacag tgacgatatc cgtttgattg
     5581 tgaagattgc ccaactctat tacgaacagg atatgacgca ggcgcaaatc gcgcgcgaac
     5641 tgggtattta ccgcaccaac atcagccgct tgcttaaacg aggccgcgat cagggaattg
     5701 tcaccatcgc catcaactat gactacaacg aaaatctctg gctggagcag caactgaagc
     5761 aaaagtttgg cctgaaagac gttgtggtgg tgtcgggaaa tgatgaggat gaagagactc
     5821 aactggcgat gatggggtta cacggcgcgc aactgctgga tcgcttgctg gagcctggcg
     5881 atattgtcgg tttttcctgg ggtcgcgcgg tgagcgcact ggttgaaaac ttgccgcagg
     5941 cggggcaatc gcggcagtta atctgcgtac caattattgg cggaccgtcc ggtaaactcg
     6001 aaagccgcta tcacgtaaac acattaacct acagcgcggc agcgaagctg aaaggggaat
     6061 cgcatctcgc ggattttccg gctctactgg ataacccatt aattcgtaat gggatcatgc
     6121 agtctcagca ctttaaaacc atctctgcct actgggataa tctggatgtc gccctggtgg
     6181 gaattggctc accggccatt cgcgacggcg ctaactggca tgcgttttat ggtggtgaag
     6241 agagtgacga cctgaatgcc cgccaggttg ctggcgatat ttgctcgcgc ttttttgata
     6301 ttcacggcga aatggttgaa acgaatatga gcgaaaaaac actctctatc gaaatgaata
     6361 aattaaagca ggcacgatat tccattggca ttgccatgag tgaagaaaaa tacagcggaa
     6421 ttattggtgc actgcgtgga aaatatatta attgtctggt aacgaatagc agcacagctg
     6481 aactattact gaaataaaaa tcttcccgat gtttatcgta ataaaaatta cggcgggtcc
     6541 ctattatcta aaatcaggag tctgttatgc aaacgtggtt aaatttgcag gataaaatca
     6601 ttatcgtcac cggcggcgca tccggtattg gtctggcgat tgtagaggaa ttattagcac
     6661 aaggcgcaaa cgtacagatg gtagatattc acggtggcga tggacaatat gaaggtcata
     6721 aaggctatca gttttggccg accgatattt ccagcgccaa agaggtaaat catacggtag
     6781 cggaaattat ccagcgtttt ggtcgcatcg acggtctggt caataacgcc ggggtcaatt
     6841 tcccgcgtct gctggtcgat gagaaagcgc ctgccgggca gtatgaactc aacgaagctg
     6901 cattcgaaaa aatggtcaat atcaaccaga aaggcgtttt tctgatgtcg caggcggtgg
     6961 cgcgacagat ggtcaaacaa catgatggcg tgattgtgaa tgtttcctca gaaagtgggc
     7021 tggaaggctc agaaggccaa agctgttacg ccgctaccaa agccgcgctc aatagcttca
     7081 cgcgctcctg gtcgaaagag ctgggtaaac acggtatccg tgtggtcggt atcgcgccgg
     7141 ggattctgga aaaaaccggg ctacgcacgc cggaatatga agaggcgctg gcgtggacgc
     7201 gcaatatcac cgtcgagcag ctgcgtgaag gctataccaa aaacgccatt cctattgggc
     7261 gcgccggaag attagcagaa atcgctgatt ttgtttgtta tctgctgtct gaacgcgcca
     7321 gctatatcac cggagtaacc actaacattg cgggcggcaa aacgcgcggc taaggaggct
     7381 ttatggtcaa cgccattttt tgtgcccacg gcaaattagc ttgcgccatg ctggaatcgg
     7441 ttcagatggt ttacggcaac gccaatgttg aagccgttga atttgtgccc ggtgaaaacg
     7501 ccggagacat cgtagcaaag ctggaaaaat tagtaagcat tcacaatcaa aatgagtggc
     7561 tgattgccgt ggatttacag tgcggcagcc cgtggaatgc ggcagcgatg cttgcgatgc
     7621 gcaatcctcg tctgcgggta atcagcggac tttctctgcc gctggcgctc gaactggttg
     7681 ataaccagga cagcatgaat gttgatgaac tgtgtgaaca cctgacgcaa atcgcaaagc
     7741 aaacctgcgt tgtctggaaa caactggcaa cgacagagga ggatttctga tgaacattac
     7801 gctcgcccgc attgatgacc gcctgatcca cggtcaggtc actaccgtct ggtcgaaagt
     7861 ggctaacgcc cagcgcatta ttatctgcaa tgacgaagtt tataacgatg aagttcgccg
     7921 gacattattg cgtcaggctg cgccaccggg aatgaaggtt aacgtggtca atattgaaaa
     7981 agccgtcgcg gtttatcaca acccgcaata tcaggacgaa accgtttttt atcttttcac
     8041 tcgcccacag gatgccttag cgatggttcg ccagggggta aaaataggca ccctgaatat
     8101 tggcggcatg gcctggcggc ccggtaaaaa acagttaacc aaagcggttt cattagatga
     8161 tgatgatatt aacgcatttc atgagttaaa taatctaggc gtcatactgg atttacgcgt
     8221 cgtagcgtca gatccctcaa tcaatattat ctacaaaata aacgaacagt taatcgcaaa
     8281 ttaaataata gcgcctgtgt acatatgctt taacaggcag ggatgacgac acttaaaagg
     8341 tgacaaatca tggaaataag taccctacaa atcattgcta tatttctttt ttcctgtatt
     8401 gccggaatgg gcagcgtgct ggatgaattt cagactcatc gcccgttaat tgcctgtacg
     8461 gtgattggtt taattctcgg tgatttaaaa accggaataa tactcggtgg tacgctggaa
     8521 ttgatagctc tcggctggat gaacgtcggc gcggcgcaat ctccggattc tgcactcgcc
     8581 agcataatct ccgccattct ggttatcgtt ggtcagcaaa gcatcgccac cggaatcgcc
     8641 atcgcgttgc ctgtggctgc ggcaggccag gtgctgacgg tgtttgcccg taccatcacc
     8701 gtagtgttcc agcacgcggc ggataaagca gcggaagaag cacggtttcg cactctcgat
     8761 attctgcatg tctccgcgct tggcgtgcag gcgctgcgcg tcgccattcc agcactgatt
     8821 gtctcgctgt tcgtcagcgc cgatatggtg agcaatatgt taaacgccat tcccgaattt
     8881 gtgacccgag gtctgcaaat tgctggcggt tttatcgtgg tagtcggtta cgccatggtg
     8941 ctacgtatga tgggcgtgaa atatttgatg cctttctttt tcctcggttt cctcgcaggt
     9001 ggctacctcg atctcagtct gctggcgttc ggtggcgtcg gcgtgatcat ggccctggtc
     9061 tacatccagc taaatccaca gtggcgtaaa gctgaacctc aactcctggc ccccgcttcc
     9121 accgcccttg accaacttga cgattaacgg agccatcatg gaacagagaa aaattacact
     9181 cggcgatctg gtgagcatgt ttctgcgctc caacctgcaa caggcgtcct ttaactttga
     9241 acgtattcac gggctgggtt tttgctacga catgatcccc gccatcaagc gactttaccc
     9301 agtaaaagag gatcaggttg cggcgctcag gcgacacctg gtgttcttta ataccacgcc
     9361 agccgtatgt ggcccggtca tcggcgtcac tgccgccatg gaagaggcgc gggccaacgg
     9421 cgcggaaatt gatgacggca ccattaacgg catcaaagtc ggtctgatgg gaccattggc
     9481 aggggttggc gatccactgg tctggggaac gctgcgcccg attaccgccg cgctcggcgc
     9541 atcgctggcg ctgtctggca acattctcgg cccgctgctg ttcttcttta ttttcaacgc
     9601 ggtgcgtctg gcgatgaagt ggtatggcct acagctcggc tttcgcaaag gggtgaatat
     9661 cgtcagcgat atgggcggga atgtgctgca aaaactcacc gaaggcgcgt cgattctcgg
     9721 actgtttgtg atgggcgtgc tggtcaccaa atggacgtca atcaacgtac cgttggtggt
     9781 ttcacaaacg catgccgccg atggctccac cgtcaccatg accgtgcaga acattctcga
     9841 tcaactttgc cctggtttgc tggcgctcgg tctgacgcta ctgatggttc gtctgctcaa
     9901 caaaaaaatt aacccggtat ggctgatttt cgccctgttt ggcttaggga ttatcggcaa
     9961 tgcgctgggc ttcctgtcct gattcttcgc cccggcacga ctgccggggc catcgttcaa
    10021 catgaggtgg tttatgaaaa caacagctct gcgtctttat ggtaaacgtg atttacgcct
    10081 ggaaaccttt gaccttcctg aaatgcagga ggatgaaatc ctcgcgacgg tggtcactga
    10141 cagcctgtgc ctctcttcct ggaaagaggc caatctgggt gaaaaccata aaaaagtacc
    10201 cgacgatgtg gcgaccaacc caatcatcat cggccacgag ttttgcggcg atattctggc
    10261 cgtgggtaaa aagtggcagc acaaattcca gccgggtcag cgttatgtga ttcaggccaa
    10321 cctgcaactc cccgaccgcc cggactgccc cggctactcc ttcccgtggg taggcggcga
    10381 ggccacgcat gtggttattc ccaacgaggt catggaacaa gattgcctgc tggcatacga
    10441 cggcgaaacc tattttgaag gctcgctggt tgaaccgctt tcctgcgtga ttggcgcgtt
    10501 caacgccaac tatcatcttc aggaaggtag ttataaccac acgatgggga ttcgcccgca
    10561 agggcgcatg ctgatcctcg gcggcaccgg accaatggga ctgttggcga ttgattatgc
    10621 gctacatgga cccgttaacc cgtcgctgct cgtcattacc gataccgaca acgataaatt
    10681 gagttatgcg cgcaagcact atccatcaga accgcaaaca ctgattcatt atctcaatgc
    10741 cgccgatgca gcatttgata cgctaatggc gctgagtggc ggtcacggct tcgatgatat
    10801 tttcgtcttt gtgcctaatg aaggactggt gactctcgcc tcttccttgc tggcgacaga
    10861 tggttgcctg aatttcttcg ccggaccgca ggataaacat ttcagcgcgc caattaattt
    10921 ctacgatgtg cattatgcat ttacccacta cgtgggcacg tcaggcggca ataccgacga
    10981 catgcgcgca gcggtcaaat tgattgaaga gaaaaaagtg caggccgcaa aagtggtaac
    11041 acatattctt gggctgaatg ccgcgggcga aaccacgctt gaattgcctg ccgtcggcgg
    11101 cggcaaaaag ctggtgtata ccgggaaata cctgccgctg acgtcactca cgcagattca
    11161 ggatcaagca ctggcggcga ttctggcgcg tcatcagggg atctggtccg gtgaggcgga
    11221 gcaatatctg ctcactcatg cagaggcaat ttcccatgat taatcgcgct acttaactct
    11281 gcatgtcgct ggctggtcac cgcggcaact tcgcgacctg atgtgcggct gattgctgaa
    11341 gcggcccaat gtgcacgaga aggataaaaa aacatcagcg ccttaagggg cgctcaaatt
    11401 ggtggcaaag tacacattgt ttatgccgga tgcggaataa agccttattc ggcctacata
    11461 atctcgaaaa ttcaatatat tgcagttatc tggtaggcct ggtaagcgta gcgcatcagg
    11521 caatgctgtg tttgtcatca gtctcagcgc ccggtgcaat acgtgacatc gtgagccatg
    11581 tcagtaagtg gttaaattcg cagtggtagc atgccttccg tgatttctta ctaaccggag
    11641 aagtaatgga actgctttta ttgagtaact cgacgctgcc gggtaaagcc tggctggaac
    11701 atgcactgcc gctgattgct gaacagttgc agggtcgccg ctcagcggtg tttatccctt
    11761 tcgctggcgt aacgcagacc tgggatgatt acacagcgaa aacggctgcg gttctcgctc
    11821 cgctgggtgt ttctgtcacc ggtattcata gcgttgtcga tcccgttgcc gcgattgaaa
    11881 atgcggaaat tgtgattgtc ggcggcggta atactttcca gttgctgaaa cagtgccgcg
    11941 agcgcgggct gctggctcca attactgacg tggttaaacg tggcgcgctg tatattggct
    12001 ggagcgcagg cgctaacctt gcttgcccaa ccattcgtac caccaacgat atgccgattg
    12061 tcgatccgca aggtttcgat gcgctaaatc tgttcccgtt gcaaatcaac ccgcacttca
    12121 ccaacgcgct gccggaaggc cataaaggtg aaacccgtga gcagcgtatt cgcgaactgc
    12181 tggtcgtcgc gccagaactg acgattattg gtctaccgga aggtaactgg atcacagtga
    12241 gtaaaggtca cgcaacgctg ggtggcccga ataccaccta tgtgtttaag gccggtgaag
    12301 aagcggttcc gctggaagct ggtcaccgtt tttaagtgaa ttgccggatg catgatccgg
    12361 catcttgttc atagtgcctg atgcgacgct tgacgcgtct tatcaggcct acaggattca
    12421 caatattaat actcgtcccg tccctcatct tcatcaggct gttccagcac actgtaagcc
    12481 accgaacaaa acagcgagtt cagacgctgc atatctccca gtaagcctaa atgtagcgaa
    12541 ctggtttcaa tgctttgcac gttttgctga tgcaggcgat cgacgtgggc gtgggaatag
    12601 cggcgattaa gaatgcgaaa gcgatgcttg ctgcggcgca aacgacgagc gctggtgaca
    12661 tcgccagaga agaacaccga cattgccagc tttaaattac tgagcaattg ctcatagagc
    12721 gcatccagtt ccttcaagcc atcaagcgaa aatgctcgcc ttgcagccag cgatttgtcg
    12781 gcaatttcac tactcatgcg ctcgacgata tcggaggcct gttcaaggtt gagcgacatt
    12841 tcgatgatct ccgcccagcg gcgcgactct tcttctgcca gttcctcttt tggcatccgc
    12901 gccagataca gcttaatggc ggtatagaga acgttgatat catctgccag cttacgcagc
    12961 tctttctcct gccttggctc gccgtgcatc actttattca gcccttccat catctgttcc
    13021 atggcgtcgc caatgcgcag ggtttcgcgc gcggcgttcg ccagagcaag cgtgggcgta
    13081 tccagcgcgc tgacatccag atgtttaggc cgtagctggg tatccagttc cggttcatcg
    13141 cgaataatcg ttttgcaaaa ccgtgccatc gggtcaacaa atggcagcat ggccaggcaa
    13201 cgtacaaggt tgtagaagac gtggaaatag atcaccagtt ccgcttttgg caatgacaac
    13261 ttccccattg tctctgccag caaatggaca aacggcagga taatcaggct acccaccagc
    13321 ttaaacagca gactacccag cgcgacacgg cgggctgcgg cattggcggc actgttgttg
    13381 agcatcgcca gcaggccgga accgaggtta gcaccaatca ccagacagag cgccacgggg
    13441 aaggagataa tgcctgcggc ggtcagagtc gcggtcagca gtacagcagc aaggctggag
    13501 tagctgataa tggcgaacat cgcgccaatc agcgcatcca gcagaatatc gccggtcagc
    13561 gaggcaaaga tcacctgaac gccgtttgcc tgggtgatcg gcgttacggc ctgcacaatc
    13621 aactccagcg ccagcaaaat cagcccaaga ccaataccga cgcggcccag ttgcccggcg
    13681 cgtgactgtt tgcgtccgag gaaaaaaatc acgccgataa aaataagtaa cggtgacagc
    13741 caggataagt cgaaggtgag aatacgcgcc attagcgccg tcccgacatc tgcccccagc
    13801 acaatgacca gagccggtgc gagggctacc agatcctgag cgacgaacga ggtcaccagc
    13861 atggtggtgg cattactgct ctgtaccagt gcggtaacgc cgatccccgc gcaaaaggcg
    13921 agcggctttt tttcgacgct ccggctaagg acagtacgca aacgcgcgcc gaagacgcgc
    13981 attacgccgg ttcgaacaat atgagtcccc cagaccagca gggcgacggc agaaagcagg
    14041 ttaagcagcg ttaacacgag ttcaggttct ccttatcgtt atatgttcct gaagcaattg
    14101 aatgaccaaa ggaccggaaa cacgctgact ggcatcttta ctgtgccttt tgacattcgt
    14161 ttttccggct aagattattc cagcctgttt ccagtataag tgtttcatat taataaagag
    14221 acagggcact cattgagcgc cctgtttgtt gtaaggaaaa gtgacagatt tgtgaatcag
    14281 tccgcgtcat accccagatt cggtgccagc cagcgctcaa catcggagac gctcatacct
    14341 ttacggcggg catagtcttc aacctgatcg cgctgaattt gcgccaccgc gtagtacttg
    14401 ctgtccgggt ggctgaaata ccaacccgaa accgatgcac cgggccacat ggcgaaagat
    14461 tctgtgagtt tcatgccggt atgtttttcc acttccagca gctcccagat ggtggctttt
    14521 tccgtatgtt ccgggcaggc cgggtagccc ggtgccggac ggatgccctg gtagttttcg
    14581 cggatcagtt cttcgttgct gaggttctcg ttcggcgcat agccccagta gactttacgc
    14641 acacgctcat ggagatactc cgcaaaggct tcggctaaac ggtcggcaag cgctttcacc
    14701 atgattttgt tgtaatcatc gtgctgcgct tcaaaggcat cagccagtgc gtcctcttcc
    14761 agcccgccag tcacggcaaa tgcgccgatg taatctgctt taccagaaag cttcggcgca
    14821 acgaagtcag cgagacagta gttagcgaat cccgtttttt cggtctgttg acgcagatgg
    14881 tggctgacgt tgatcacatg ggtgcgcgtt tcgtcacggt agatttcaat gtcatcgccc
    14941 acacggtttg ccgggaacag gcccaccacg ccacgcggat tcagcgtttt ctcggcgctt
    15001 aatttatcca gcatgtcgtt ggcgtcttta aacagccgct gcgcctcaac gcccaccact
    15061 tcatcttcca gaatgcgcgg atacttcccg gccagcgacc aggtcataaa gaacggtgtc
    15121 cagtcgatgt aattacgcag agtttcgatg ctggcttcaa cttcctgcac gccgagacgg
    15181 tgcgccaccg gcggcgtgta agcctgccag tcaaaagcga agtcgttatc gcgcgccgct
    15241 tccagcgtga ccggtggtgt gcgtggtttc ttgcgcccgt gctgaatacg tacggtttcg
    15301 tactccttgc gggtacgagc gacaaaatca tcgcgctgag tatcggaaag cagcgccgcc
    15361 accacaccaa cggtgcgcga ggcattctgc acatacaccg tcgggccgct gtagttctgc
    15421 tcgattttca ccgccgtgtg cgcttttgag gtcgtcgcgc caccgatcag taacggaatg
    15481 gtgaagccct gacgctccat ctctttcgcc acgttaacca tctcgtccag cgacggcgtg
    15541 ataagccccg aaagtccaat cagatcagca ttcacttctt tagcggtacg gagaattttt
    15601 tccgcaggca ccataacgcc gagatcgaca atttcgtagt tgttacattg cagcaccaca
    15661 ccaacgatat ttttaccgat gtcgtggacg tcgcccttca cggtggcgat caccatcttg
    15721 ccgttggttt tgccctgctc tttgctggct tcaataaacg gttcgaggta ggccactgcc
    15781 tgcttcatca cacgcgccga tttgaccacc tgtggcagga acattttccc ttcgccgaac
    15841 agatcgccga ccacgttcat gccgtccatt agcgggcctt caatcacttc aatcgggcgc
    15901 gtagcctgct ggcgggcttc ttcggtatcc tgctcgataa actcagtaat gcctttgacc
    15961 agcgagtatt ccagacgttt attcacttcc cacgagcgcc actccgcctg ctgggcgtta
    16021 gcgatgtcgt cggttttgct gccgcgatat ttctcggcaa gctccagtaa acgctcagtg
    16081 ccatcatcgc gacgattaag aatcacatct tccaccgcgt cgcgcagttc agcgggcagg
    16141 tcgtcgtaaa tcgccagttg cccggcgttg acgatcccca tatccatgcc attgcgaata
    16201 gcgtagtaga ggaacaccgc gtgaatggct tcgcgcaccg gatcgttgcc acggaatgag
    16261 aaagaaacgt tagatacgcc gccggaaatc agcgcgtgcg gcagttcgcg tttgatgtct
    16321 tcgcaggcgc cgataaagtc ctgcgcatag ttgttgtgct cttcaatgcc ggtggcgacc
    16381 gcgaagatgt tcgggtcgaa gatgatatct tctggcggga agccaacctc ttcggtgagg
    16441 attttgtacg cccgacggca aatctcaatt ttccgtgcgc gggtatcggc ctgcccctgt
    16501 tcgtcaaagg ccattaccac caccgctgca ccgtaacgac gcaacagttt cgcatgatgg
    16561 ataaaggcgt cgacgccctc tttcattgag atagagttaa caatgccttt gccctgaata
    16621 cacttcagcc ccttttcgat gacctcccat tttgaggagt cgatcataat cggcacgcga
    16681 gcgatatccg gttcaccggc aatcagattg agaaaacgca ccatcgccgc ttcggcatcg
    16741 agcatccctt catccatgtt gatatcgata atctgcgcgc cgttttccac ctgctgacgc
    16801 gctacatcca gcgcctcgct gtatttctct tctttgatca ggcgcttgaa cttagcggaa
    16861 ccggtgacgt tggtgcgttc acccacgttc acaaacaggc tatcgtcgcc aatgttcagc
    16921 ggctccaggc cggacaaacg gcaggctacg ggaatttcag gcagtttgcg cggcgctaat
    16981 ccttctactg cacgactcat cgctgcaata tgttgtggcg tggtgccaca gcagccgccg
    17041 acgatattga gaaaacccgc ttgcgcccat tcacgtatct gttttgccat cgtgtcggcg
    17101 tcgagatcgt actcaccaaa ggcgttgggt agcccggcgt tcgggtgcgc ggtgacgtag
    17161 cattccgcaa tccgtgatag ttcctgcacg tactggcgca gttcatctgg ccccagcgcg
    17221 cagttgaggc caaaagtcag ggcttcggca tggcgcaggg agttgtaaaa tgcttcggtg
    17281 gtctgtccgg agagcgtgcg cccggaggcg tcggtgatgg tgccggagat cataatcggc
    17341 agctcaacgc ctaacgcttc gaactccgtt ttcaccgcaa agaccgccgc tttggcgtta
    17401 agggtgtcga aaacggtttc aatcagaatc agatccgcac cgccttccac taatgctttg
    17461 gtggattcgc gataggccgc caccagctgg tcaaaagtga tgttgcggaa tgccggatcg
    17521 ttcacgtccg gagaaatgga cgccgtgcgg ttggttgggc cgagaacgcc ggcaacgtag
    17581 cgcggtttct ccggcgtgcg cgcggtccac tcgtcagcac aggcgcgcgc cagtttcgcc
    17641 gccgcaaagt tgatttccgc cgacagggat tccatctggt aatccgccat cgcaatggtc
    17701 gtggagttga aggtgttggt ttcgatgata tccgcgcccg cttcaaagta ggcgttgtgg
    17761 atagcggcga tcacttccgg tttgcttagc accagcagat cgttattgcc tttgaggtcg
    17821 catggccagt cggcaaagcg ttcaccacga aaatcggctt cgttcagtcg atagctctgg
    17881 atcatggtgc ccataccgcc gtccagcacc agaatacgtt catttaactg cgcacgcagt
    17941 tgttccactt tgctgctcac acttgctccc gacacgctca acccagattt aataaaaatt
    18001 caacaaacca tactggcata aacgcatctg tggtaaaagc gaccaccacg caacatgaga
    18061 tttgttcaac attaactcat cggatcagtt cagcaactat tgcattagct aacaataaaa
    18121 atgaaaatga tttccacgat acagaaaaag gagactgtca tggtcgcacc cattcccgcg
    18181 aaacgcggca gaaaacccgc cgttgccacc gcaccagcga ctggacaggt tcagtcttta
    18241 acgcgtggcc tgaaattact ggagtggatt gccgaatcca atggcagtgt ggcactcacg
    18301 gagctggcgc aacaagccgg gttacccaat tccacgaccc accgcctgct aaccacgatg
    18361 caacagcagg gtttcgtgcg tcaggttggc gaactgggac actgggcaat cggcgcacat
    18421 gcctttatgg tcggcagcag ctttctccag agccgtaatt tgttagcaat tgttcaccct
    18481 atcctgcgca atctaatgga agagtctggc gaaacggtca atatggcggt gcttgatcaa
    18541 agcgatcacg aagcgattat tatcgaccag gtacagtgta cgcatctgat gcgaatgtcc
    18601 gcgcctatcg gcggtaaatt gccgatgcac gcttccggtg cgggtaaagc ctttttagcc
    18661 caactgagcg aagaacaggt gacgaagctg ctgcaccgca aagggttaca tgcctatacc
    18721 cacgcaacgc tggtgtctcc tgtgcattta aaagaagatc tcgcccaaac gcgcaaacgg
    18781 ggttattcat ttgacgatga ggaacatgcg ctggggctac gttgccttgc agcgtgtatt
    18841 ttcgatgagc accgtgaacc gtttgccgca atttctattt ccggaccgat ttcacgtatt
    18901 accgatgacc gcgtgaccga gtttggcgcg atggtgatta aagcggcgaa ggaagtgacg
    18961 ctggcgtacg gtggaatgcg ctgacttttt ctggcgggca gaggcaatat tctgcccgtc
    19021 atacctgagt ggtaatagaa taagggtgtc tgttaatcgc attgacgtca aaataactta
    19081 ttgtcataca cttcatacac ttttattaac tcagcattat ttttaaacat caaaccactt
    19141 aattattaac aaaatcgcaa gatctcattc caccgaaact tttacggctg cattatgaga
    19201 ttgagcgatt tatgatattt ctttagttgt aatcatatta
//
LOCUS       DAZFAB010000086        19167 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZFAB010000086 DAZFAB010000000
VERSION     DAZFAB010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.086"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..112)
                     /locus_tag="ACHJWA_003460"
     CDS             complement(<1..112)
                     /locus_tag="ACHJWA_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4309990.1"
                     /translation="MSRHDILLRSQFERIIEGDRVGQALISFYEKLPEENY"
     gene            complement(258..4160)
                     /gene="hrpA"
                     /locus_tag="ACHJWA_003461"
     CDS             complement(258..4160)
                     /gene="hrpA"
                     /locus_tag="ACHJWA_003461"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFO4309991.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRIGHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWFNKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            4361..4966
                     /gene="azoR"
                     /locus_tag="ACHJWA_003462"
     CDS             4361..4966
                     /gene="azoR"
                     /locus_tag="ACHJWA_003462"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFO4309992.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTTRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(5017..6309)
                     /gene="ynbD"
                     /locus_tag="ACHJWA_003463"
     CDS             complement(5017..6309)
                     /gene="ynbD"
                     /locus_tag="ACHJWA_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HFO4309993.1"
                     /translation="MLQGAGWLLLLAPFFFFTYGSLNQFTAVQDFNSHDIPSQVFGWE
                     TAIPFLPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATVMACCGFFLYPLKF
                     SFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIVLCWLLWRHFRQHLAVRWRKVCGGW
                     FLLIAISMLTTWQHHFIDVITGLAVGMLIDWMIPVDRRWNYQKPDQRRIKIALPYVVG
                     ACACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQGKLPPAVYW
                     LTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTFEFPRGRAT
                     KDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSALVVAAWLL
                     CYGHCKTVDEAISFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(6323..8080)
                     /gene="ynbC"
                     /locus_tag="ACHJWA_003464"
     CDS             complement(6323..8080)
                     /gene="ynbC"
                     /locus_tag="ACHJWA_003464"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFO4309994.1"
                     /translation="MENSRIPGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHIVDELAMPDTAFYAWDARGHGQTSGPRGYSPSLARSVRDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYHRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFISRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGT
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPGNEQVKNSLAGLAN
                     AIEPGGILLYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQGEMDSLVRDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(8096..8992)
                     /gene="ynbB"
                     /locus_tag="ACHJWA_003465"
     CDS             complement(8096..8992)
                     /gene="ynbB"
                     /locus_tag="ACHJWA_003465"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO4309995.1"
                     /translation="MLEKSLATLFALLILATLINRFRVWRLPERKGDEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFLALKEYCTLISVHFPRWLYWVIPLNYLLIGFSC
                     FELFLLFIPLAGFLILTTWRVLVGDTSGFLHTVSAIFWGWIMTVFALSHAAWLLMLTT
                     TNTQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     ASLIIGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAVKRDVGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            complement(8992..9597)
                     /gene="ynbA"
                     /locus_tag="ACHJWA_003466"
     CDS             complement(8992..9597)
                     /gene="ynbA"
                     /locus_tag="ACHJWA_003466"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO4309996.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLITGL
                     LLMLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGIRSYVGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLLMSAER"
     gene            complement(9768..12074)
                     /gene="ydbD"
                     /locus_tag="ACHJWA_003467"
     CDS             complement(9768..12074)
                     /gene="ydbD"
                     /locus_tag="ACHJWA_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HFO4309997.1"
                     /translation="MFKPRLCSWIGLLPLFMLSLPVQAELRCVANAVDIEPFFSAATA
                     EDKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGNLTVKGNIS
                     TFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSTIKGSINARGI
                     FINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWGLINDEDALKEIF
                     KRSNIRMAGNVCNQMKKEALFRLKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIMR
                     TLPRLKGISANLRKQLINSNDGQTIESMARYMPDNEILELTDQQLGYQPVVLGLLDRE
                     PLSVEIMTRMSHLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPES
                     IIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNDVVTSLALKLAEMKHHGQLLRM
                     TPQESDKIAVYLYQKFENDDDLIGALFLALPDNLQFNFVKRMEKKSPAYFCCRDMQII
                     HSDAALQRLLTRFNDPEGGSNLAKNQYLSTSMKQKIWQRALSHRKNNPKADSDAYETS
                     ADMILSELISYGEVDDQMLLNATSLIRSDDWDFLESALISWDNLPAVVLKELQQNTPR
                     NDIWAKFFLRQENSSRAQVNEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLT
                     FFKFGALNNRHTPPAVLAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALKLVNPE
                     LDLVRQLALNGKTRAIREQAMRKLDELY"
     gene            complement(12138..12998)
                     /gene="pdxI"
                     /locus_tag="ACHJWA_003468"
     CDS             complement(12138..12998)
                     /gene="pdxI"
                     /locus_tag="ACHJWA_003468"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HFO4309998.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIEALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEKV
                     LSTLDGISRE"
     gene            complement(13218..15677)
                     /locus_tag="ACHJWA_003469"
     CDS             complement(13218..15677)
                     /locus_tag="ACHJWA_003469"
                     /inference="COORDINATES: protein motif:HMM:NF015737.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO4309999.1"
                     /translation="MNEGGALTNEGVLNTVTLEGGTFNNNGTLNDVVKIEKNSNAVIN
                     NTGSLSTLQLHDGTVNNSGIASARVNAQGDAVFNNLAGGEARKGAILYNSAVVNNAGT
                     WKMGYQDENNNAGTLDIDDKSTFNNSGKLILDNSKNAIRFQGSNANATLYNTGEMTLD
                     AALGAGAIHYDDGASEFINKGVVDAKVTVAVSTAGATESDAFLWNQDGGVINFDKDNA
                     SAVKFTHNNYVALNDGVMNISGNNAVAMEGDKNAQLVNNGVINLGTEGTTDTGLTGMQ
                     LDANATADAVIENNGTINIFANDSFAFSVLGTEGHIVNNGTVVIADGVTGSGLIKQGD
                     SVNVEGVNGNSGNNTEVHYTDYTLPDMPNTYTTSPFSETTDSGSSDGSSNNLNGYIVG
                     TNVDGSAGKLKVNNASMNGVGINTGFAAGTADTTVSFDNVVEGINLTDADAITSTSVV
                     WTAKGSTDASGNVDVIMSKNAYTDVATDASVNDVAKALDAGYTNNELYTSLNVGTTAE
                     LNSALKQVSGSQATTVFREARVLSNRFSMLADAAPKVGNGLAFNVVAKGDPRAELGNN
                     TEYDMLALRKTVDLSESQSMSLEYGIARLDGDGAQKAGDNGVTGGYSQFFGLKHQMSF
                     DNGMRWNNALRYDVHNLDSSRSVAYGDVSKTADTDVKQQYLELRSEGAKTFEPREGLK
                     ITPYAGVKLRHSLEGGYQERNAGDFNLSMNSGSETAVDSIVGLKLDYAGKGGWSANAT
                     LEGGPNLSYSKSQRTASLAGAGSQHFNVDDGQKGGGINSLASVGVKYSSKESSLNLDA
                     YHWKEDGISDKGVMLNFKKTF"
     gene            complement(15897..18932)
                     /locus_tag="ACHJWA_003470"
     CDS             complement(15897..18932)
                     /locus_tag="ACHJWA_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed virulence protein BigA"
                     /protein_id="HFO4310000.1"
                     /translation="MQRKKLLSVCVAMALSSQTWAADTSIPDSTEKTRKSSKITCPAN
                     IHSLSKEQLENLSAECRENKDSAVLPWAAAGLAAVATGIAIYTLSDDDNHHHNNSPVP
                     DDGGDTPVPPDDGGDTPVPDDGGDTPVPPDDGGDTPVPPDDGGDTPVPPDDGGDTPVP
                     PDDGGDTPVKHNPVVYKNGVTWDQDAKTVKIRETTFTYSQNADGSYTLTAPEGKETIV
                     KSWTVHDDSNTVVFDGVNTSGGITWSYDDDGLIHITKEAGVVVDGTTGNAIEFGNAII
                     TDQGGNTALNGGTVMTVDGDNISLNNDGKTTAIGEGSVVGILTGDNITINNNGETEVD
                     GGTAVIINGDNTKLNTAGDSTITNGGTGSLINGDNARVDNQGTMSVDGENSTGSKIVG
                     DGATIKQEGDLYVSGGAHGIDVDGNDTFVSNKGNITVIEDNSIGMLLDGDGVSVINMG
                     DLNVGQAAAGENAIGIQIDGDNATFVNVGDISATNAGTGVSVAGDKANISLAGGLDVG
                     DFSTGLDVSGNNNNMTLATYELNVTGQKATGVNVSGDGNTIEIAGSILVDKDQKADNA
                     QPYFFNPSTGVNISGDNNDVTLDGQLTVVADSKTTSRSYASYDGAQEHIAGIVIAGDD
                     NTFTLNGGVHFVGEKNVMDDGSKPSASRRGIGDTPLINVDGHSPVYLNGESTISGEFP
                     LGFENLIQLSHGAELEIGADATFDMSDVDSFTYYYRVALSTISIDSGAKATNNGEVEL
                     KNIGFAAAWNKDSTVINNGSIGLAMYDFGTDPAPKVFDVEYGGIGVNNGTMTTKMMNQ
                     HSVLNYPAEWNLSDGTSFNNKALGLTGMLASYSSSILNGETGIIDMYGRGSVGMLAID
                     KSTADNEGQITLDTLWVDENDETSLRNNVANSTAKDFGVGMASGTDAYNGALTKATAT
                     NHENGVITVYNAGAGMAAYGNTNTVINQGTINLEKNENYNDSLGVNKLVGMAVYHEGT
                     AINDQTGVININAENGQAFYNDGTGLIINYGTICTFGVC"
BASE COUNT         4869 a         5138 c         4313 g         4847 t
ORIGIN      
        1 ggtaattttc ctctggaagt ttttcataga aagatattaa agcctgacca acgcgatcac
       61 cctctattat ccgttcaaat tgcgacctca gtaaaatatc atgtctactc attgcaatac
      121 gtcctgttcg cccaaggcga agtatgaatg aaacttatgt aaaatagtac cgcccttgct
      181 atatttcaga attcataaag aaaaaacccg gcgctaaatg cttaccgggt tttagattta
      241 tcaggcaaat agcagggtta accgctaatc tgctccatcg cctgcaaaat acgcttatct
      301 gaaatcggat aaggcgtacc aagttgttga gcgaagtaac taacgcgcag ctcttctatc
      361 atccaacgga tctctttcac gtcttcatcc tcacgacgtg caggcgggag tttgttgaac
      421 cattgctgcc acgcctgctg gacgttttcg actttcagca tctgtgcacg atcgcgatgt
      481 ggatcaaccg ccagtttttc cagccgtttt tcaatcgcct gcaaataacg cagcgtgtcg
      541 cccagccgtt tgaagccgtt accagtgaca aaaccgcgat ataccaaccc gcccatctgc
      601 gctttaatgt cagaaagccc cagcgccatg gtcatatcca cccgcccttt cagacgtttg
      661 ttgatattga acactgccgt aaggatttgc tcgacctgct tcgcaatatc caccaccgtg
      721 tcgttcagtt cggcacgcac tttttcatgc agcgcagcaa agccttcttc cgtccagacc
      781 gggcctccat tggcgtcgat cagttgatcc acaccgcatg agatacagtc gtcgatcagc
      841 tccagcactt tgccatacgg gttaaagtac agccccagct tggctttgtt tggtaacttc
      901 tcatgtaaat acttgattgg tgagggaata ttcagcagca gtagacggcg aagaccgttc
      961 cacattgcct gtttttgctc cagcgggtta tcaaacagtt tgatcgccac actgtcgcgc
     1021 tcatccacca gcgccggcca cgccttcact ttgtagttac cacgcttctg ttcgtagctt
     1081 tccggcaact gaccaaaact ccagatatgt aagccgctct gctcgatacc gtcatccgcc
     1141 accgcagata gcgtttcctg cactttgcct ttcagcgcat ctttcagatc ttgtagcgag
     1201 cgcccttctt ttagcttctt gtttttgtca tccaccacgc ggaaggtgat tttcaggtga
     1261 tcgggcacct gatcccagtg ccagtcttcg cggtcaacgg taacgccggt catccgccgt
     1321 aactcgcgct caaggctgtc gagcaacggt aactccagcg gtgtgacgcg gcctaaaaac
     1381 gcttcggcat agtttggcgc aggtacaaaa ttacggcgta ccggtttcgg caacgattta
     1441 atcagagcaa tcaccagttc gcggcgcaga ccagggatct gccattcaaa cccgctttcc
     1501 tcaacctggt taagtaacgg cagcggaata tgtacggtca caccgtcagc atccgcgccg
     1561 ggctcaaact gatagctcaa acgcagcttg agattgccct gatgccagaa gttcgggtaa
     1621 tccagcttgc tgatcttttc cgccccttct ttgatcaaca tgctcttttc aaagttgagc
     1681 aaatcaggcg tttcgcggct gacttttttc caccagctgt cgaagtgacg agcggagatt
     1741 acatcgtggc cgatgcgctg gtcgtagaac tcaaacagcg tttcgtcatc aaccagaata
     1801 tcgcggcgac gtgatttgtg ttcaagctct tccacttcgg cccgcagttt caggttttca
     1861 cggaagaatg cgtgacgcgt ctgccagtca ccttccacca gcgcgtggcg aataaagagt
     1921 tcacgacata acgccggatc gatctggctg tagttgacct tgcgcgcggc aacaatcggc
     1981 aaaccataaa cagtgacttt ttccgttgcc atcaccgcgc cctgcgcccg ttcccagtgc
     2041 ggttcgctgt aggtacgttt aatcaaatgc tgagcgacag gctccaccca ttccgggtcg
     2101 atacgcgcag caatgcgccc ccacaggcgg ctggtttcta ccagttccgc caccattacc
     2161 catttcggcg gttttttgaa taaaccagaa ccggggaaga tggagaaacg cgcgttacgt
     2221 gcgccggtat attcttgttt atcggcatct ttcatgccga tatgggaaag caaccccgtc
     2281 agcaacgcaa tgtgaatttc acgatactcc gccggttcgc tgttcaccgg aatgccgagt
     2341 tctttcacca cctggcgcaa ctgggtgtag atatcctgcc attcgcgcac acgcagatag
     2401 ttgagataat cggtacgaca caggcgacgg aaggcgttgg aagaaagcgc cttttgctgc
     2461 tcgccaagat aattccacag attcacaaac gcgaggaagt cagactcttt gtcgtggaag
     2521 cgacgatgtt tttcgtccga tgcctgctgt ttgtccatcg gacgttcgcg cggatcctga
     2581 atggagagcg cagacgtgat aatcatcgcc tcacgcacgc agccatgttt ttgcgcttcc
     2641 agcaccatac gcgccagacg tgggtcgaca ggcaactgcg agagctggcg accgagcggc
     2701 gtcagtttat aggcgctggc ctgttcatcc gtggtgatcg cgcccagttc ttcgagcaga
     2761 cgcacgccat cctggatatt gcgtttatcc ggtgcttcga caaacgggaa cgcagcgata
     2821 tcgcctagcc ccagcgcggt catctgcaaa ataaccgagg ccaggttggt acgcagaatc
     2881 tccggatcgg taaactccgg gcgcgagagg aaatcgtctt cggaatagag acgaatacag
     2941 atcccttcgg acacacgacc acagcggcct ttacgctgat tggcagacgc ctgcgaaatg
     3001 ggctcaatcg gcaaacgctg cactttggtg cgatagctgt agcggctgat acgcgccgta
     3061 ccggggtcga taacgtattt aatccccggt acagtcagcg aggtttccgc gacgttggtc
     3121 gccagcacaa tgcgtcgccc gctgtgcgac tggaacacgc ggttctgttc gctattcgaa
     3181 agccgcgcat aaagcggcaa gatttcggta tggcgtaagt tcagcttgtt cagcgcatcg
     3241 gcggtatcgc gaatttcccg ctcgccgctc ataaagatca gaatgtcgcc ggggctttcc
     3301 tggcttagtt cgtccacggc atcgaaaatt gcctgcaact ggtcgcgctc agtgtcgtcg
     3361 gcttcttcaa caatcgggcg atagcgcact tccaccggat aggtccgacc ggagacttca
     3421 ataatcggcg cattattaaa gtggcgcgaa aagcgttccg ggtcgatagt cgcggaagtg
     3481 ataatgattt ttaggtcagg acgccgcggc agcaactctt tcaaataacc gagcagaaaa
     3541 tcgatattca ggctacgttc gtgcgcttcg tcaataatga tagtgtcgta ctgcatcagc
     3601 aggcggtctt gctggatctc cgccagcagg ataccgtcgg tcattagctt gaccatcgtg
     3661 ttatcactta cgtgatcgct gaaacgcact ttgtaaccga tgcaaccgcc cggctccgtt
     3721 ttcagctctt ccgcaatacg gttcgctacc gttcttgccg ccaggcgacg cggctgggta
     3781 tggccgatca gccctttaat cccgcgcccc agctccatac agattttcgg taactgagtc
     3841 gttttaccag aacccgtttc cccggcgact atcaccacct ggtgatcacg aatcgcttcg
     3901 agaatgtcct gttttttctg actaaccggc aaattgtcag gataagtaat ttccggtcgt
     3961 gccgcttcac gcagcaggac tttccctgcc gcctggtcaa tctctttcgc catctcctgg
     4021 aaaatggcct gttgtgcatc aggattttta accttcttca cgccgtgcag acggcgagaa
     4081 aaacgcagtc tgtcacgcag catcagcgaa tccagccgct gctgcaaggc cgtaaaagtc
     4141 aatttttgtt gttctgtcat aacgttagtg ggcagtagtc tgcctgatta attttctttg
     4201 aaatgataga tatagagtac cacatcgcgg ctttctatgc gttgttcaat aaattcgaac
     4261 ataggcttcg atttattgcg ctatctctgt ggcaggattg tgaataaagt gtcaacaagc
     4321 aacggggcat cgcccattca aatatctata aggaaacacc atgagcaagg tattagttct
     4381 taaatccagc atcctggcag ggtactctca gtctaatcag ttgtccgatt attttgttga
     4441 acaatggcgc gaaaagcact ccgctgatga aatcaccgtt cgcgacctgg ctgcaaatcc
     4501 aattccggta ctggatggcg aactggttgg cgctctgcgt ccgagcgatg cgccgctgac
     4561 tacgcgtcag caggaagctc tggcactttc cgatgagctg attgccgagc tgaaagccca
     4621 tgacgttatc gttattgcgg caccgatgta taacttcaac atctcgactc agctgaaaaa
     4681 ctattttgac ctggttgcac gtgcaggcgt tactttccgc tataccgaga atggtccgga
     4741 aggtctggta acgggtaaaa aagccatcgt tattaccagc cgcggtggga tccacaaaga
     4801 tggaccaacg gacctggtga cgccgtatct gtccacgttc ctgggcttta tcggcattac
     4861 cgatgtgaaa tttgtcttcg ccgaagggat cgcatacggt ccggaaatgg cagcgaaagc
     4921 gcagtctgac gcgaaagcag ccatcgacag tattgttgct gcataaccgg acacgatctt
     4981 gatgtggact gtagttcagt ccacatctca atccacttac ctgttttccc ataatttcaa
     5041 catcgctttg tgctcttcct taagcacaat atgcgagcgt ctggctcgaa taaagctaat
     5101 cgcttcatca actgttttac agtgtccgta acataacagc catgccgcca ctaccagcgc
     5161 actgcgcgat aatcccaatg cgcaatggac cagaacgctg ccttgctctt cgcgtaatgt
     5221 ttccagcatc gccacggcct gtcggagctc cccctcttcc ggaaccacca gatccagcat
     5281 cggtacacag aaatagagtc gatcttttgt cgctcgtccg cgagggaatt caaaggtgac
     5341 atccagaacc gcattctgtg ccggaatatg tcgtggaaac gcccctaaat aaacaccagc
     5401 agtcattttg ctcaccggct ccaggcgacg acaaaaccaa cgcatagata gccacatccc
     5461 gatgcgccag ggcaatgtca gccagtaaac ggcgggcggg agtttcccct gactatcttt
     5521 ccctgttgtt atcgcgccaa gcccaccgta cccacggcca ataatgagta gcgataatac
     5581 tggccaacat aaccagactg accaccataa ctgaaccatc atcattagct ccatcaacac
     5641 aatgcacgcg cacgcgccta cgacatatgg cagtgctatt ttgattcgac gttgatcagg
     5701 tttctgataa ttccaacgac ggtcgacggg tatcatccag tcaatcaaca tacctaccgc
     5761 cagccctgtg atgacatcaa taaaatgatg ctgccaggtt gtcagcatcg aaatggcgat
     5821 gagtaaaaac catccgccgc agactttacg ccacctcaca gccagatgct gacgaaagtg
     5881 acgccaaagt agccagcaga gaacaatatg cagcgaagga gactggttat aaggcagatc
     5941 aaacagttca agttgcgaaa atagccatcc tgtcacccca ctcacttcag gacggataaa
     6001 actaaacttc agcgggtaga gaaaaaaacc gcagcaggcc attaccgttg ccagaataag
     6061 ccggtggaca agtcggcgct gttcgaatgt cgagctacaa acgaacagcg aaaatccata
     6121 taaaagatcc agactccagt aagggacaat agtccaggga agaaaaggga tcgccgtttc
     6181 ccagccgaat acctgactgg ggatatcatg gctgttaaag tcctgaaccg cggtgaactg
     6241 atttagagat ccataggtga agaagaaaaa cggggccagc aacaataacc agccagcgcc
     6301 ttgtagcaat acgttccggc gttcagttat cacgacgcac cgccatcgaa accgtaaaaa
     6361 tgccccactc atcaatccgt tgtgtgcatt tatcaaatcc ggcatcacgc acgagtgaat
     6421 ccatctcccc ttgcgaacgc acgcgcatta cccacggttt accatcttta tgactggtta
     6481 acaccccggc aatcagctcc agttgtgggt gccacggctg cccggtgtag aggagaatgc
     6541 cgcccggttc gatggcattg gcaagacctg cgagtgagtt ttttacctgc tcatttccgg
     6601 gaaaaagctc atacagtcca gagacaatcg ccagcgtagg ccgcggagtt aacgcgctga
     6661 gttcctccgg gttaaacgca tcgccctgtt caaaacgcac ccgcccagac attccccgtt
     6721 gagcaatcat ctcttgcccc tgtgcaacat ttaactcgct gtaatcacgt aacaaaatat
     6781 cgcttacggc aggctcgttt gccagcgcat ccagtacata gcgcccatgc cctgcggcaa
     6841 tgtcaaccac gcggacggct aaacctttgg cgtggagatc ggcaacggct tgtttaatca
     6901 gtatttgtaa atgggttttg cgctggcgaa taccgcgcca gccaacactg ttcaggtaga
     6961 ttttgtcgat taagcgcccg aatgcgttac taccttgcgg ttgattttga tagacataat
     7021 ccagcgaact gcctgaatca aagccggtgc tcattccgag atgcaggccc gcagagtgcg
     7081 tcccgaacaa tttcatcgct ttacgcataa agcgatacgc caaatcaacc ggcgataatg
     7141 gcacgggtcc acctgaaagg agccgccagc gatccgctga tggtccagtg cggtcttcat
     7201 gctgataatc aaatttttgt gacttgttag catataaacg actaataaag ctttgcattt
     7261 tttcaaatgc ctgcgccctg ttctcttcac ccaacgtgtc gtgataaaag cctggcagca
     7321 gatgcagctc tttcagaggg ctacgtaatc tatgataaaa atcaatctgc ggttggcgat
     7381 gcaccacata gtcatcgcct gatatcagaa gttgcgtggg gagcgtaatc gccgccgcat
     7441 cgctaacaat acgttcagac gttttataaa gatcgagcaa gatattaacg gcaatcgccc
     7501 gtgtgatcag cggatcatta ttgaaactcg ccacccgttg ccgatcgtgg gtcaaatagc
     7561 gtcctttcac ataggaatta ataaaaaaca gaccacgcag acgatgccat aacgccagcg
     7621 caggacgtgc cagcggcaca tacaatttaa ccttaaaggc cggagaagcc agcaccagcc
     7681 cgcgaattgc aggcgcataa tcatgaaccc atgtggcaac cagcactgcg ccgacgctct
     7741 gcgcgatcac taccacctct tccagcccga cctggctgtc gctggcagca aaacggacaa
     7801 actcatcgac atcccgcact gaacgcgcaa gagatgggct ataaccacgg ggccccgaag
     7861 tttgtccatg ccctcgggca tcccatgcat aaaaagcagt atctggcatc gccagttcat
     7921 caacgatatg ttgtagacga ccagaatgtt catgaccgcg atgaaataag acgatgacct
     7981 ttttcgcccc tggctgtaaa gcgggccagt gccgataaaa caacgctgta ttatcactgg
     8041 tggtaaaaaa atgttcccca gggatgcgtg aattttccat tttctctatt ttccttcagt
     8101 aacagcagta gcgaataaaa taaaaaaata ccggagcggt gaaaattaat gagtcaatcc
     8161 tgtcaaggag tccgccatgt cctggtaata gttttccgct atctttaaca cccacatctc
     8221 gtttgacggc tgacatcact acatcgccgc aaaaaccact aataccaatt aacaaacccg
     8281 ctaataatgc ctgtaatgta tttagcggtg tcagcaacgg tccaataatc agtgacgcaa
     8341 tcatggtggt gattacgccc cccaccagac cttccagcgt ttttcccggg ctgactttgg
     8401 ggaccacttt tcttctgccg caggatttcc cccataaata ctgtgcaata tcgtttgact
     8461 ctgttaacgc caaaagaaat aacaccagta acgccccgcc ctgggtattt gtggttgtca
     8521 acattaataa ccaggcggca tgactcaagg cgaaaacggt cattatccag ccccaaaaaa
     8581 tggcgctcac ggtatgcaga aaaccggagg tatcacctac taaaactcgc caggtggtta
     8641 atatcagaaa cccagccaaa ggtataaata acagaaacag ctcaaagcaa ctaaaaccga
     8701 taagcaaata gttaagagga ataacccaat ataaccaacg cggaaaatgt acggatataa
     8761 gcgtacaata ttctttcaat gccagaaaac ttatcagcgc aaaaaacgtc aacgtcatcc
     8821 agcgtggacc tgaaatcacc agtgaaaaac aaatgacgat accccaccag gtacgaatac
     8881 gtagtgtaac ttcatcccct tttctctccg gtaaccgcca tacccgaaaa cggtttatca
     8941 gcgttgctaa aattaacaac gcgaaaagtg ttgccagaga tttttccagc attatctttc
     9001 agcactcatc aggagcacac tccgacaacg attaatcgca gtccagagaa gcagaattga
     9061 agcaatgctc cataaaagat tattccattg catccactgt ggataaatgg caacggccag
     9121 cccccacaac ccaaatatta acgcgcgatc gcttttgcca aaaggaccga cataactacg
     9181 aataccgtta atcgtctgcg cgagtaaacc gcaaaactca gtcaatatgg tgcaaaataa
     9241 catgagtatt acgagtgatg cgttactttc cggtaaaaat aaaaacggta aatagagcgc
     9301 aatatcggaa ataacatcgc cagtttcatt taaaatcgcc cccagacgtg tttgctgatt
     9361 gcactcacgc gccaacatgc catccagcgc attgagtgcc atacggataa aaagcacgat
     9421 gggcaatagc aaaaagagga tgggttgtgc gaccaacatc aacagcaatc cggtaataag
     9481 agaaagcgcc agtgcagcaa gagtgatgtg attcgctgta acatggtgct tataaagcca
     9541 aaacatcgtc ggccttaaca gcgactgaaa gagcggtttt atctggtaga gcgtcattat
     9601 caatccttga taattcggtt tgcgctatgg tgcatcaatt cccctgacgg agatatcgca
     9661 acacgacgct tcattaaaaa ttcagaatat atcaaggtat tggggactca ctgtcaggaa
     9721 tgattatccc caataaatca agtggttaaa aattattacg ctgttattca atacaactca
     9781 tcaagttttc tcatcgcttg ttcccgtatt gcgcgcgtct taccgttaag cgccagctgg
     9841 cgaactaaat ccagttcagg gttaacaagc tttaacgcca gcaaaggatc acgtttcagc
     9901 ctcgccttta atacatcaac tggaaaacgg ggattattca tcgccacaat ccaccactcg
     9961 ggatcaattt ctgccgccag tactgcaggc ggtgtgtggc ggttgttaag tgcgccgaac
    10021 ttgaaaaatg tgagattgct tttcgccagt gtactccacc atatacgatc cggttgtttt
    10081 gccagtacat ccagttgtgc taacgcatcg gggtctagtg cataataaac acgtaacgct
    10141 tcgttaacct gcgcacggga actgttttcc tgcctcagaa aaaacttcgc ccaaatatca
    10201 ttgcgtggcg tgttttgctg taattccttg agaacaacag cgggtaaatt atcccaacta
    10261 attaatgcac tctctaaaaa gtcccagtcg tccgaacgta tcagagaggt ggcatttaat
    10321 agcatctggt catcaacttc accgtagctg ataagctcag acaaaatcat atcagcgctg
    10381 gtttcatatg catctgaatc tgctttggga ttatttttcc gatgcgataa agcacgttgc
    10441 caaatttttt gtttcatcga cgtgctcaga tactgatttt tcgccagatt actccccccc
    10501 tcaggatcgt tgaaacgtgt caataaccgt tgtaaagcag cgtcagagtg aattatctgc
    10561 atatcccgac agcaaaagta ggcaggggat tttttctcca tccttttaac aaaattaaac
    10621 tgtaagttat ctggcaacgc taaaaataac gcacctatta gatcatcgtc attctcgaat
    10681 ttttggtata aatatactgc tattttgtca ctctcttgtg gcgtcatccg caacaattga
    10741 ccatggtgtt tcatctctgc cagctttaac gctaagcttg tgacaacatc gtttgatgaa
    10801 tcgttcgcca actgcattgc ctgattgtag gtgagttgtc ctccagcacc gacctgacga
    10861 atagaggaat catcactgcg gatatagcca tcaataatgg attcgggcaa taaccacgca
    10921 tctttataaa gctcctgaat gatcatatcc caatctcttt tcgccagagt catgacgata
    10981 tccagaggga gattttcacg tagcgcaaga ttcaacggac cgacaccatc aggtagatga
    11041 ctcattcgcg tcattatttc gacagagagc ggttcacggt cgagcaaccc cagaacaaca
    11101 ggctggtaac ccaattgctg atcggtcaac tccagaatct cattgtcagg catatatctc
    11161 gccatacttt caatcgtttg tccatcattg ctattaataa gttgtttgcg taggttagct
    11221 gaaattcctt taagccttgg gagagttcgc attatggcaa gatcaaatgt cgcaatatct
    11281 cgtccttcaa atgcagcaac gttgccttca tccagcattt gcaattcctg cactaactca
    11341 ggagaaggct taaggcgaaa cagcgcttct tttttcatct gattacagac attccctgcc
    11401 atgcgaatat tgcttcgctt aaaaatttct ttcagagcat cttcatcatt tattagaccc
    11461 caataatatg catcaggatt tattatcatc agcgcttttt tgacattttc agtgtcattt
    11521 ttgtcactcg cacggaccat gaattcacta tttgtcgaag acgcaacaac aggtgcaatt
    11581 atgtcattga taaatatccc acgcgcatta atactcccct taatcgtaga cggattatcg
    11641 taatatgagt cgataaatac cagtccactt gcatttatcg aaccggtaat taacagtggt
    11701 gaatccgtaa ctatattcgt tgccgtcacg ttaccgagaa taacccatgg attagaaaga
    11761 gagaaagtgg aaatattacc tttgactgtc aaattgccaa gaacaatcaa tttttgatta
    11821 ctctctatat taccttctac cactaaatcg ccacgatgca ctttccagtc cgatgcagat
    11881 aaaccgaagg ggacaagatt cacgctactg ttgatagctt gttcaacttg ttgtttatct
    11941 tcagcggtag ccgcagaaaa aaaaggttca atatcaactg catttgcaac gcatcgaagt
    12001 tccgcctgta ctggaagact cagcataaac aggggaagta aacctatcca gctgcaaagc
    12061 ctgggcttaa acatatgcac tccatgccat taaaaaagaa acaaaaggga aatgcatctt
    12121 tcccttttgt atattcgtta ttctcgcgaa ataccatcca acgtagacaa cactttctca
    12181 gaaagatgca acttttcagc agccatattc tcccgtaaat gcgcaacaga agacgtccct
    12241 gggatcagca aaatattcgg tgaacgttgt aacagccacg ccagcgccac ctgcattggt
    12301 gttgcaccca agctcgcagc aacatcggaa agcgtggaag attgcagcgg cgtaaagccc
    12361 ccgagcggaa agaacggcac gtaggcaatg ccgtcacgag ccaaagcttc aatcattgcg
    12421 tcatcagcac gatgagcaat gttgtattcg ttctgcacac agacgatttc ggcaatcttg
    12481 cgcgcctctg caacctgcgt cggtgtgacg ttgcttaggc caatatgttt taccaggcct
    12541 tgttgttgca tctctgccag cacggtcagg ctggcttcaa tcgatccttc cgccgggcca
    12601 tgaccatccc ccatcatcac gcgcaggtta accacatcca gtacgtccag gccgagatta
    12661 cgtagattat cgtgcaccgc tttttgcagc tctgccggag aaaatgcggg caaccaggat
    12721 gcgtcctctc cacgccgcgc accaatttta gtgacaattg tcagatcgtc agagtaagga
    12781 taaagcgctt cgcggataat ctgattggtg acgtgcggac cataaaagtc gctggtatca
    12841 atgtgattga cgcccaatgc cagcgcctca cgcagcacgg ttatagcgac gtgacgatct
    12901 cttggggggc caaaaacccc aggacctgcc agttgcatcg cgccataacc aagacggtta
    12961 acggattttg taccgagagt aaatgtattg ctgctcatcg caccctgccc tgtaataaaa
    13021 cggattaaga aaaatcagta tagaaacgaa aagagataaa taatgcgaag gcgtaaacaa
    13081 gatttctgga tgagtagcac tattgtcctg aaagacacca aaacattaga cagcaaaaga
    13141 taagtcaagc gtcgcatccg gcaataaaaa accgcaacct gtatcagcgt tgcggtttgt
    13201 gtatcacatg ctaaaaatta gaacgttttc ttaaagttca gcatcacgcc tttgtcgctg
    13261 atgccgtcct ctttccagtg atacgcatcc agattcagcg aactttcttt gctactgtac
    13321 ttcacgccga cgctcgccag gctgttgata ccgccgccct tctgaccgtc atcgacgtta
    13381 aagtgctggc tgcctgcccc tgcaaggctt gccgtgcgct ggctcttgct gtagctcagg
    13441 ttcggcccgc cttccagcgt cgcattcgcg ctccagccgc ctttccctgc gtagtccagt
    13501 ttcagcccga cgatgctgtc caccgccgtt tcgctgccgc tgttcatgct caggttaaag
    13561 tctccggcat tgcgctcctg atagccgcct tccagcgagt gacgcagttt cactccggcg
    13621 tacggggtga ttttcagccc ttcgcgcggc tcaaaggttt tcgccccttc gctacgcaac
    13681 tccaggtact gctgtttcac atccgtatcc gccgttttgc tgacgtcgcc gtaagcgacc
    13741 gagcggctgc tgtcgagatt atgcacgtca taacgcagcg cgttgttcca acgcatacca
    13801 ttgtcgaagg acatctggtg cttcaggcca aagaactggc tgtagccgcc ggttacgcca
    13861 ttgtcgcccg ctttctgcgc accgtcgcca tccagacgcg cgataccgta ttccaggctc
    13921 atgctctggc tttcgctcag gtcaacggtt ttacgcagtg ccagcatgtc atactcggtg
    13981 ttatttccga gttccgcacg cgggtcacct ttcgccacca cgttaaaggc caggccattg
    14041 cccaccttcg gcgcggcatc cgccagcatg ctgaagcggt tgcttaacac acgcgcttca
    14101 cggaataccg tggtcgcctg gctaccgctc acctgcttca gggcgctatt cagttcagca
    14161 gtggtgccca cgttcaggct ggtatacagc tcgttattgg tgtaacccgc atccagtgcc
    14221 ttcgccacat cgttcaccga agcatcggtc gccacatcgg tgtaggcgtt tttgctcatg
    14281 atgacgtcaa cgttgccgct ggcatcggtg ctgcctttgg cggtccatac cacggacgtt
    14341 gaggtgatgg catcggcgtc ggtcaggttg atgccttcca ccacgttgtc gaaactgacc
    14401 gtggtgtctg ccgtaccggc ggcgaaaccg gtgttgatcc cgacaccatt catgctggcg
    14461 ttgttgacct tcagtttacc ggcgctgccg tcaacgttgg taccgacgat atagccgttg
    14521 aggttgttac tgctgccatc gctactaccg ctatcagtcg tttcactgaa aggtgaagtg
    14581 gtgtaagtgt ttggcatgtc cggcaacgtg tagtcggtgt aatgcacttc ggtattgtta
    14641 ccgctgttgc cgttcacccc ttccacattc acgctgtcgc cctgcttaat caaccccgaa
    14701 ccagtcacgc cgtcggcaat caccaccgta ccgttgttaa cgatatgacc ttctgtgccc
    14761 agtacgctga acgcaaacga gtcgttagca aaaatattga tcgtgccgtt gttctcaatt
    14821 accgcatcgg cggtggcatt ggcatccagt tgcataccag tcaatccggt atcggtggtg
    14881 ccttcggtac cgagattgat aacgccgttg ttaaccagct gtgcgttttt atcgccttcc
    14941 atcgccacgg cgttgttgcc gctgatgttc attacaccat cgttgagagc aacatagttg
    15001 ttgtgggtga atttaaccgc gctggcgttg tctttatcga agttaattac cccgccatcc
    15061 tggttccaca agaaggcatc gctttctgtc gcaccggcag tacttaccgc cacggtgact
    15121 ttcgcatcca ccacgccctt gttaataaac tcgctggcgc catcgtcgta gtgaatagcg
    15181 cccgcaccta atgcggcatc cagcgtcatt tcaccggtgt tatataacgt ggcgttagca
    15241 ttgctgccct ggaagcgaat ggcgtttttg ctgttatcaa ggatgagttt gccgctgttg
    15301 ttgaacgttg acttatcgtc aatatccagc gtcccggcat tgttgttttc atcctgataa
    15361 cccattttcc aggtacccgc gttattcact accgcagagt tatagagaat cgcgccttta
    15421 cgcgcctcgc cgcctgcaag gttattgaat accgcatcgc cctgagcgtt aacgcgcgcc
    15481 gacgcaatac cgctgttatt caccgtacca tcgtgaagtt gtaaagttga cagggaaccg
    15541 gtgttattaa ttaccgcgtt gctgtttttc tcaattttta cgacgtcatt aagcgttccg
    15601 ttgttattga aggttcctcc ttccagagtc accgtgttca atacaccttc attggttaac
    15661 gcaccgcctt cattcacgac gatggctgag ctaatggttc cgctcgactc gttaaccagc
    15721 gcaccatttt cgagtatcct gatcgttcca tttttaacag cacccgcact ggtgacatcc
    15781 ccgttgataa tcgcacctga cgataataat acggtctcac ccgcagaact taatacagcg
    15841 ccatcatcat ataaatcagc aatataatta tccgttggat tatattcatc ggccccctag
    15901 caaacgccaa aggtacaaat tgtgccatag ttgataataa gcccagtgcc atcgttatag
    15961 aatgcctgac cattttccgc gttaatgttg attacgcctg tctggtcatt aatggcggtg
    16021 ccttcgtgat aaaccgccat gccgacgagt ttattcacac cgagagaatc gttgtaattt
    16081 tcatttttct ccaggttgat ggttccctgg ttaatgaccg tattagtatt cccatacgcc
    16141 gccatccccg caccggcgtt gtagacagta atcacaccat tctcgtggtt ggttgccgtg
    16201 gctttagtta atgccccatt ataggcatcg gtacctgaag ccattcccac accgaaatct
    16261 ttcgccgtgc tatttgcaac attattacgc agcgatgttt catcattttc atcaacccac
    16321 agcgtatcca gggtgatttg cccttcgtta tccgcggttg atttatcgat ggctagcatc
    16381 cctacactgc cacggccata catatcaata atacccgttt cgccgttcag aatggatgac
    16441 gagtagctgg caagcatacc tgtcagccct aatgccttgt tattgaaaga ggtaccatca
    16501 gacaagttcc actctgccgg ataattcagc acactgtgct gattcatcat tttggtggtc
    16561 atggtaccat tgttaacgcc aatcccaccg tattcaacat caaagacttt tggtgccggg
    16621 tcggtaccaa aatcgtacat cgccaggcca atggatccgt tattgattac cgttgaatct
    16681 ttattccagg cagccgcaaa accgatattt ttcagttcca cttcaccgtt gtttgtcgcc
    16741 ttcgcaccag aatcaatact aatggtggat aacgctacac gataataata agtgaaagaa
    16801 tccacatcac tcatatcaaa cgttgcgtca gcgccaatct ccagctccgc gccatggctc
    16861 aactgaatga gattttcgaa ccccagcggg aactcgccgc tgatggttga ctcaccattc
    16921 aggtagaccg gggaatgccc atcaacgtta ataagcggtg tgtctccgat accacgtcga
    16981 gaagctgatg gttttgagcc atcatccatg acattttttt caccgacaaa atggacaccg
    17041 ccattaagcg taaaggtatt gtcatcgcca gcaataacaa tacctgcaat gtgttcctga
    17101 gcaccgtcgt aactcgcata ggaacgcgac gtggttttac tatcagcaac aacggttaac
    17161 tgaccatcca gcgtgacgtc gttgttgtca ccgctgatat ttaccccggt tgagggatta
    17221 aagaagtaag gctgcgcatt atcggctttc tgatctttat caaccagaat actgcccgct
    17281 atttcgatag tattaccatc acccgaaaca ttaacacccg tcgctttttg ccctgtcaca
    17341 ttcagctcat aagtcgccag cgtcatgttg ttattattgc cagagacatc aagaccggtg
    17401 gagaaatccc caacgtctaa cccacccgcg agtgaaatat tagctttatc cccagcaacg
    17461 ctgacaccgg ttccggcatt ggtcgcggaa atatcaccaa cgttaacaaa tgtcgcatta
    17521 tcaccgtcga tctgaatgcc aatggcattt tcacccgctg cggcctgacc aacattgagg
    17581 tcgcccatgt tgatcacgct gacgccatca ccatcaagca gcatgccaat agagttatct
    17641 tcgatgaccg taatattacc tttgttgcta acgaacgtat cattgccgtc aacgtcaata
    17701 ccgtgcgcac caccgcttac gtataagtcg ccttcctgtt tgatggtggc accgtcaccg
    17761 acaattttcg agccagtgga attctctccg tcaacgctca tggtaccttg attatcgacc
    17821 cgcgcattat cgccattgat aaggctaccc gtaccaccat tggtgatggt cgagtcgccc
    17881 gcagtgttta gcttggtatt atcgccattg atgattaccg cggtaccgcc atcaacttcc
    17941 gtctcgccat tattgttaat ggtgatattg tcgccagtga gtataccgac aaccgagcct
    18001 tctccgatgg ccgtcgtttt gccgtcgttg ttgagggaga tgttgtcacc atcaaccgtc
    18061 atgacagtac cgccattcag cgccgtgttg ccgccctgat cggtgatgat cgcgttacca
    18121 aactcaatcg cattcccggt tgtgccgtca acaacgacgc cggcctcttt agtgatgtgg
    18181 atgagtccat catcgtcgta actccaggtg atgccacctg aggtatttac gccatcaaag
    18241 acaacggtgt tgctgtcgtc atggactgtc caggacttaa ctatcgtctc cttgccctca
    18301 ggcgccgtta aggtgtagct cccatcggca ttttggctgt aggtaaaggt agtttcgcga
    18361 attttgacag tttttgcatc ctgatcccag gttacgccat ttttatagac gacaggattg
    18421 tgtttaaccg gggtatcgcc accatcgtct ggcggaactg gcgtatcacc gccgtcatct
    18481 ggcggaaccg gcgtatcacc gccgtcatca ggcggaactg gcgtatcacc gccgtcgtct
    18541 ggcggaacag gcgtatcacc gccatcatcc ggaactggcg tatcaccgcc gtcgtctggc
    18601 ggaacaggcg tatcaccgcc atcatccgga actggggagt tattatggtg gtgattgtca
    18661 tcgtcactta aggtatatat agcaattccc gtagctactg cagcaagacc tgcagctgcc
    18721 catggtaaaa ctgcactatc tttattttct ctgcactccg cggataaatt ttctagctgc
    18781 tctttgctca gggaatgaat atttgccgga catgttatct tactggactt acgtgttttt
    18841 tcggttgagt cgggtattga agtatccgca gcccatgtct ggctacttaa cgccatggca
    18901 acacaaacag acaataattt tttcctttgc atataaaatt ccttagatct agaccgtgcg
    18961 taacaagcca tgcagaattt gaagataaat cgtagtaaat attcatagtg aaataagcag
    19021 taaagaaatg cttatataat tcacaaaatc aatattaaaa tcaggcaagt aaaagcatac
    19081 aaaaaaaaca gttgatggaa ttatattcca caaaaaactc acttcaatct aaaaattaac
    19141 gacaaaaaac aaaaacacac taataat
//
LOCUS       DAZFAB010000087        19153 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZFAB010000087 DAZFAB010000000
VERSION     DAZFAB010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.087"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(293..1009)
                     /locus_tag="ACHJWA_003471"
     CDS             complement(293..1009)
                     /locus_tag="ACHJWA_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012773552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFO4310001.1"
                     /translation="MPIMQIGSLHLLVNRKAIKNLHISVLPPDGKVRVSAPEHMTETA
                     IRMAVASRFRWIRKQQQDFARQPRQSEREMVSGECHYLWGRRYRLLVIERHGRHEVKV
                     AGNNKLQLYVQPGTRDENKALALSEFYRHELKQQVAKLLPEWQDRIGVPLTFWGIKKM
                     KTFWGTCNTTTKRIWLNLELIKKPPECLEYILVHELVHLLERHHNARFRLHMDRFIPD
                     WRERRDLLNNMPLAFEAWEY"
     gene            complement(1009..4104)
                     /locus_tag="ACHJWA_003472"
     CDS             complement(1009..4104)
                     /locus_tag="ACHJWA_003472"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015872650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFO4310002.1"
                     /translation="MSTVGQRERATQQRLIQFFVEDLGYRHLSHRTGYTGNSNIDTNI
                     LSAWLKRRGVNDTLITRALRQLDSAAALGEGKKLYYANKEVYRLLRYGVKDKEGAGEQ
                     NQTVWLIDWANPQANDFAIADEVTVKGEYCKRPDLVLYVNGIALGVIELKRSSVHISE
                     GIRQNLDNQKKTFIRDFFTTMQLVMAGNDTQGLRYGTIETPEKYYLEWKEDNPGDYTH
                     KLDFHLSRLCSKSRLLDIVHNYIAFDVGVKKLCRHNQFFGVEAAKQHIARREGGIIWH
                     TQGSGKSLTMVWLAKWIRENVPNSRVLIVTDRTELDEQIESVFMGVNEDIYRTSSGND
                     LIATLNHPNPWLICSLVHKFGRRSEAEDNAATDAFITELQQSLTKTFRAKGDLFVFVD
                     ECHRTQSGKLHNAMTAILPDALFIGFTGTPLMKKDKKKSVEVFGPYIHTYKFDEAVAD
                     GVVLDLRYEARDIDQYLTSEKKVDDWFEAKTRGLSNLARTQLKQKWGSMQKLLSSKSR
                     LEQIVNDILLDMDTRPRLMDGRGNAMLVCSSIYQACKVYEMFSQTELAGKVAIVTSYR
                     PDAASIKGEETGEGLTEKLSKHATYRKMLADYFEQSEEKAAARITEFEREVKQRFIEE
                     PGQMRLLIVVDKLLTGFDAPSATYLYIDKTMTDHTLFQAICRVNRLDGEDKEYGYIID
                     YKDLFRSLDKAITDYTTGAFDDYDRDDVAGLLKDRLEQAQLDLDSALEMVRSLCEPVK
                     TPRSLQDYQHYFCGESGENQTSLNEKEALRLTFYQCVARLLRAYASIANEMPEAGYTA
                     EEVESIRAEVAEFEKLRYEIKLTSGDLLDMKRFEPAMRHLLDMYVRADDSEVLMDFEE
                     LSLIELIVEKGLAATVALPDDIRNNQDAMAETIENNVRKTIVDENPVNPKYYGRMSQL
                     LDELITLRRQNALNYQQYLERIRDLCKQVIRPEQTAGACYPATMDTQAKRAFYDNFGH
                     DEVLATRIDTTIRYTKRAEWIGDRFKEREIANALREETASYNIDIDEVIALARQQKEY
                     H"
     gene            complement(4123..5190)
                     /gene="darG"
                     /locus_tag="ACHJWA_003473"
     CDS             complement(4123..5190)
                     /gene="darG"
                     /locus_tag="ACHJWA_003473"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin DNA ADP-ribosyl
                     glycohydrolase DarG"
                     /protein_id="HFO4310003.1"
                     /translation="MITYTQGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPENMK
                     VYALACKQKQVITGKMFITETGELMGPRWIVNFPTKQHWRADSRMEWIEDGLQDLRRF
                     LIEENVQSIAIPPLGAGNGGLNWPDVRAQIESALGDLQDVDILIYQPTEKYQNVAKST
                     GVKKLTPARAAIAELVRRYWVLGMECSLLEIQKLAWLLQRAIEQHQQDDILKLRFEAH
                     YYGPYAPNLNHLLNALDGTYLKAEKRIPDSQPLDVIWFNDQKKEHVNAYLNNEAREWL
                     PALEQVSQLIDGFESPFGLELLATVDWLLSRGECQPTLDSVKEGLHQWPAGERWASRK
                     LRLFDNNNLQFAINRVMEFHC"
     gene            complement(5187..5843)
                     /gene="darT"
                     /locus_tag="ACHJWA_003474"
     CDS             complement(5187..5843)
                     /gene="darT"
                     /locus_tag="ACHJWA_003474"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system toxin DNA ADP-ribosyl
                     transferase DarT"
                     /protein_id="HFO4310004.1"
                     /translation="MAYDYSASLNPQKALIWRIVHRDNIPWILDNGLHCGNSLVQAEN
                     WINIGNPELIGKRAGHPVPVGTGGTLHDYVPFYFTPFSPMLMNIHSGRGGIKQRPNEE
                     IVILVSNLRNVAAHDVPFVFTDSHAYYNWTNYYTSLDSLDQIDWPILQARDFRRDPDD
                     PAKFERYQAEALIWQHCPISLLDGIICYSEEVRLQLEQWLFQRNLTMSVHTRAGWYFS
                     "
     gene            complement(5856..7205)
                     /locus_tag="ACHJWA_003475"
     CDS             complement(5856..7205)
                     /locus_tag="ACHJWA_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO4310005.1"
                     /translation="MVDLMVNEQRSGTAGKDVPAGYKLTEVGVIPEDWVVISIDDCTT
                     KVGSGKTPTGGASVYIDSGRPFVRSQNVDWGRLDLADVVHITDETHNTFSGSEIQQDD
                     VLLNITGASIGRCAKADFRVIGGNVNQHVCIIRTEAKKLSPSLLVELINSNYGQNQID
                     SYQAGGNREGLNFSQVKNLKFAITPNSFEQTEIVRTLNDINAFTLALENLIAKKQAIK
                     AAAMQQLLTGKTRLPQFALRKDGSAKGYKKSELGEIPEDWNCINLGELGNCIIGLTYK
                     PEDTSDYGTLVLRSSNIQNNHLAYDDNVFVSIEPPERTIVKEGDILVCVRNGSRQLIG
                     KCALIDKNAQGSAFGAFMSVFRTDIYGFTFHQFQSDIIQKQIAEVMGATINQITNKDM
                     LGFKIPLPENTEEQAAIAAILSDMDKEIQILQQRLDKTRQLKQGMMQELLTGKIRLI"
     gene            complement(7205..9643)
                     /locus_tag="ACHJWA_003476"
     CDS             complement(7205..9643)
                     /locus_tag="ACHJWA_003476"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFO4310006.1"
                     /translation="MAIKKTELYSSLWASCDELRGGMDASQYKDYVLTLLFMKYVSDK
                     YKGDPYGMIVIPKGASFDDMVALKNDKEIGDKINKIIHKLAEENGLKGVIDEADFNDE
                     DKLGKGKEMIDRLSKLVGIFEGLDLSSNRVEGDDLLGDAYEYLMRHFATESGKSKGQF
                     YTPAEVSRILAKVIGITPDTPRDATVYDPTCGSGSLLLKVNDEARRGLSIFGQEMDNA
                     TSALARMNMILHNNATAKIWQGNTLSDPQWKEANGKLKAFDFAVANPPFSNKNWTNGL
                     TPKKDPFERFGWGIPPEKNGDYAFLLHIIKSLKSTGKGAVILPHGVLFRGNAEANIRE
                     NLIKQGYIKGVIGLPANLFYGTGIPACIIVIDKEHAHSRKGIFMIDASRGFIKDGNKN
                     RLRSQDIHRIVDVFNHQRTVPGYSRMVPSSEIAGNDYNLNIPRYIESGEPEDLHDLTA
                     HLQGGIPVRDVDALKDYWRVFPALRNVLFADDHPGYCRAQVDAQQVKPTILAHQEFKD
                     FATRSLLPFKSWVKEAGLEEIRKGDKPKALIHDISEMLLAQYANSELLNKYSVYQILM
                     DYWADVMQDDVYVIMQDGWKAAAQIRELQPVKGKDGKNIWKETHDFEFTKRRYKADVL
                     PRSLVEIRCFPELLDALNRAQERSEEASRQLVEFIEEQAGEEDLLAEVKNDKDKVTQK
                     LVNARLAQLKPPPADPDELAVLNRCLALIKAEASAKKALKAAQEALDRAVFKHYPTLD
                     EAAIKTLVVQDKWLATLQAGIKAEIERITQQLASRVKELEERYAEPLPALEAAVEALS
                     EKVAGHLRAMGLEW"
     gene            complement(9683..9859)
                     /locus_tag="ACHJWA_003477"
     CDS             complement(9683..9859)
                     /locus_tag="ACHJWA_003477"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310007.1"
                     /translation="MNMNEDEIYRHIRQALSSAPRNQYTVELHLQMIKYADELEHITA
                     KAFCEGTGLNTDLL"
     gene            10872..12251
                     /locus_tag="ACHJWA_003478"
     CDS             10872..12251
                     /locus_tag="ACHJWA_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310008.1"
                     /translation="MYNNLYALTESLFWVDRLLEQEYENIGLNELLVELAEYNKRIDS
                     FIIEQTNSVQESRLNVYMAGNRNIERYKSGLFSMDRVILDDVLYSLSLRYRVNLSENV
                     VEKFIESDSKEKVEHLRKEIARFIRFAKENFDLIRGEFIVFVRRESSIKDSERANGIL
                     EDNSDTNFLFRYLPPNIARLYESRLSVQNIERLGNTNKIRLLNRNVLTNEIMLKIKGC
                     ESSYTNGYMYQHINNAIDNDDGTMSMEIVMGKHSGKKSYERWVQGAKNRTVLFHYKNL
                     ITDLNQAHSCRASLGAYCPFHGEILRKLDQRNEVQRVKLSIDVPFLEGLTPSELYKIR
                     TDYESSFNAFRSVLREAAYDIEAESDPYKRQLINQHFVERIWDEGLTDIEQKIQTYKR
                     KSNKELFLNIAPSLIGFIGAPNPVSLATGAFTILKEVFSITSHSSEIKSHPSYFLLMT
                     SKGKNKFYD"
     gene            complement(12388..12906)
                     /locus_tag="ACHJWA_003479"
     CDS             complement(12388..12906)
                     /locus_tag="ACHJWA_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310009.1"
                     /translation="MTILEEMEAEAWHILTTIYTYQRLMEGLTNSSTRYELANQLVAL
                     NALLEMLVIRLARLADKRKDVRSVSMFLKRGSYSASPEAVKLAAEKFLALTEPVLKIR
                     YEQIAHMKPGTLSSFEPRELPNEALRATEALIDLIDIARGHPQSYTYRVGSQEPAINL
                     RASVETSELVKI"
     gene            13262..14134
                     /locus_tag="ACHJWA_003480"
     CDS             13262..14134
                     /locus_tag="ACHJWA_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO4310010.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVPGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLNGHGHSMVITDLPGVGESRDRDA
                     EYETLYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTDAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELCTESVRGQAREQFTGAVDRIFDTVESVCIASVARTVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            14507..17356
                     /gene="cah"
                     /locus_tag="ACHJWA_003481"
     CDS             14507..17356
                     /gene="cah"
                     /locus_tag="ACHJWA_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding autotransporter Cah"
                     /protein_id="HFO4310011.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRTGVAVALSLAA
                     VTSVPALAADTVVQAGETVNGGTLANHDNQIVFGSTNGMTISTGLELGPDSEENTGGQ
                     WIQNGGIAGNTTVTTNGRQVVLEGGTASDTVIRDGGGQSLNGLAVNTTLNNRGEQWVH
                     EGGVATGTIINRDGYQSVKSGGLATGTIINTGAEGGPDSENVSSGQMVGGTAESTTIN
                     KNGRQVIWSSGVARDTLIYAGGDQTVHGHALNTTLNGGYQYVHKDGLALNTVINEGGW
                     QVVKAGGAVGNTTINQNGELRVHAGGEATAVTQNTGGALVTSTAATVTGTNRLGHFSV
                     GNGMADNVVLENGGRLDVLEGHSAQNTLVDDGGTLAVSAGGKATDVTMTSGGALIADS
                     GATVEGTNASGKFSIDGISGQASGLLLENGGSFTVNAGGQAGNTTVGHRGTLTLAAGG
                     SLSGRTQLSKGASMVLNGDVVSTGDIVNAGEIHFDNQTTQDAVLSRAVAKGDSPVTFH
                     KLTTTNLTGQGGTINMRVRLDGSNTSDQLVINGGQATGKTWLAFTNVGNSNLGVATTG
                     QGIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYA
                     SMLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGS
                     YGFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLN
                     LVHTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQ
                     LQYTWQGLSLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMTFGEGTSSRDTLRDSA
                     KHSVRELPVNWWVQPSAIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEAR
                     IRENITLGVQAGYAHSVSGSSAEGYNGQATLNMTF"
     gene            17467..>19153
                     /locus_tag="ACHJWA_003482"
     CDS             17467..>19153
                     /locus_tag="ACHJWA_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HFO4310012.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVDEPDVLSDSKNENGLL
                     FDKRKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLITLQEKLATPAGQQVAQSLQQTGDTRELL
                     DILADDVWLKNEYHGEDEIFTGLASLNDLVRLAAAMGTEFPFDEYAEVQKLPVIDVAF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEMMMRDQLQKASAVLAVMDYTQMNSKADE
                     EVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNGALPVDEAWVDDFIREAFGPMVEEDDWKDSTKVNKKAEKL
                     WNISLIDQLITEVIQSSHSRAAALAVDSAAAKLMQNAENVSEYLSLRHQGLQQSIQSL
                     QAHITSLLADIQEIEECQNQVTGDVRMAMEDINTKTGELLTKVCASLEEELNDYFRSG
                     KRKEQQMLEEENSAQPRERNAFAFFHDIFGTGNQHDRMRDFDPDSPEIKFSDRR"
BASE COUNT         4712 a         4799 c         4751 g         4891 t
ORIGIN      
        1 cgaccgccag gctggtatcg gaggcgggtt ctgaagaact gcgtattacc gtgaaggaga
       61 tcggtgagta accccctgtg tcagcatagt tgtcgcctca attttttaag gagataacca
      121 tgctagtaag cgttaacgga gcgccgtatt gatgcttact tattggtgag tactacgttc
      181 catggcagga gttgagcctg tacatagatt tgtgtaattg tctgattttg atatgttcaa
      241 tccagcatca aatgaaggtt aatttatgga caaaaaacag ttacaggctc aatcaatact
      301 cccatgcctc aaacgctaac ggcatattat ttaacaaatc ccgacgctca cgccagtcag
      361 gaataaaccg atccatatgc aaccggaagc gagcattatg gtgacgttcc agaagatgaa
      421 ccagttcatg caccagaata tactccagac actccggcgg cttcttaatt agctccagat
      481 tgagccagat tcgttttgtt gtagtgttac aggtgcccca aaaggttttc atctttttga
      541 tgccccagaa tgttagggga acaccgattc ggtcctgcca ttcaggtaga agcttagcca
      601 cctgctgttt tagttcatgt cgataaaatt cacttaacgc caatgcctta ttttcatcac
      661 gggttcccgg ttgaacataa agctggagct tattatttcc cgcaactttt acttcgtgac
      721 gcccgtgacg ctcaatcaca agtaggcgat atcttcggcc ccaaagatag tggcactctc
      781 cgctgaccat ttccctctct gattgacgag gctgtctggc gaaatcctgc tgctgttttc
      841 tgatccagcg aaagcgtgaa gcaaccgcca tacgaatcgc cgtctccgtc atatgttccg
      901 gcgctgaaac gcggactttg ccatcaggcg gcagcacgct gatatgcaga ttcttaatcg
      961 ccttacggtt gaccagcaag tgtaacgagc ctatttgcat gattggcatc agtgatattc
     1021 cttctgctga cgggcaagcg ctatcacctc atcaatatca atattatagc tggcggtctc
     1081 ttcacgtaat gcgttggcaa tttcccgttc tttaaaacga tcgccaatcc actctgccct
     1141 tttggtatag cgaatcgtcg tatctatgcg ggtcgccagt acctcgtcgt ggccgaagtt
     1201 atcatagaag gcgcgctttg cttgtgtatc catggtggca gggtagcacg ctccggcggt
     1261 ttgctccggg cggatgactt gtttacacaa atcgcggata cgttccagat actgctgata
     1321 attgagcgca ttttgtcggc gaagggtaat cagttcatcc agcagctgcg acatgcgtcc
     1381 atagtatttt gggttaaccg ggttttcatc cacgatggtt ttgcgcacgt tgttttcgat
     1441 ggtttccgcc atcgcatcct gattattacg gatatcatcc ggcagggcga cggtcgccgc
     1501 caagcctttc tcgacaatca gttcaatcag acttaactct tcaaaatcca tcagcacttc
     1561 gctatcatcg gcgcgaacat acatatccag taaatgacgc atagcgggtt caaagcgctt
     1621 catatccaga agatcgccgc tggtcagctt aatctcatag cgcagttttt cgaattcagc
     1681 gacttcggcg cgaatgctct ctacctcttc ggcagtgtag cctgcttccg gcatttcatt
     1741 ggcgatgctg gcataagcgc gtagcagacg ggcgacgcac tggtaaaatg tcaggcgcag
     1801 cgcctctttt tcgtttaacg aagtctgatt ctctcctgat tcaccgcaaa aatagtgctg
     1861 atagtcctgc aggctgcgcg gggttttgac gggttcacat aagctgcgaa ccatctccag
     1921 cgcgctgtcg agatccagtt gcgcctgctc cagcctgtct ttcagcaacc cggccacatc
     1981 gtcccgatcg taatcatcaa acgcgcccgt ggtgtaatcg gtaatcgctt tatccagcga
     2041 acggaacaga tctttataat cgataatata gccatactct ttatcctcac cgtccagccg
     2101 gttcacccga caaatcgcct gaaacagcgt atggtcggtc atggttttat caatgtagag
     2161 ataggtggcg gacggcgcgt caaagccagt cagcagctta tccaccacga taagcaggcg
     2221 catttgcccc ggctcttcaa taaaacgctg tttcacttcc cgctcgaact cggtaatgcg
     2281 tgcggcggcc ttctcttcac tttgttcaaa atagtcagcc aacattttac gataggtggc
     2341 gtgtttagag agtttctccg ttaacccttc gcccgtttct tcgcctttga tgctggcggc
     2401 atcggggcga tagctggtca caatcgctac ttttcctgcc agctcggtct ggctgaacat
     2461 ctcgtaaacc ttgcaggctt gatagatact gctgcatacc agcatggcgt tgccgcgccc
     2521 gtccatcagg cgtggacggg tatccatatc cagcaaaatg tcgttaacga tctgctccag
     2581 ccgcgacttg ctggagagca gcttctgcat tgatccccac ttttgcttaa gctgggtcct
     2641 ggctaaattc gacaaaccgc gagtcttggc ttcaaaccag tcatcgacct ttttctctga
     2701 tgtcagatac tggtcaatat cgcgtgcctc gtagcgcaga tccagcacca caccgtcggc
     2761 caccgcttca tcaaatttat aggtatggat ataagggccg aacacctcaa ccgacttctt
     2821 cttatctttc ttcatcagcg gtgtgccggt aaaaccgata aacagggcgt ccggcagaat
     2881 ggcagtcatg gcgttatgca gcttgccgga ttgtgtgcgg tggcactcgt ccacaaaaac
     2941 aaacagatcg cctttggcgc ggaatgtttt cgtcagggac tgctgtaatt cggtgataaa
     3001 ggcgtcggta gccgcattgt cttcagcttc gctgcgacga ccaaatttat gcaccagcga
     3061 gcaaatgagc cacgggttag gatgattgag cgttgcgata agatcgttgc cgctggaggt
     3121 gcgataaata tcttcattca cgcccataaa cacgctttcg atctgttcat ccagttcggt
     3181 gcggtcagtc acaatcaaca cgcgggagtt tggcacattt tcacggatcc acttcgccag
     3241 ccagaccatc gtcaggcttt tacccgatcc ctgtgtgtgc cagataatac cgccttcacg
     3301 acgggcaata tgttgtttag ccgcctcgac gccgaagaac tgattatggc ggcagagttt
     3361 tttcaccccg acatcaaagg caatgtaatt atggacgata tccaacagcc tggatttgct
     3421 acacagacga cttaaatgaa agtcgagttt atgtgtatag tcgcctggat tatcttcttt
     3481 ccactccaga tagtattttt ccggggtttc gatggtgccg tagcgcaggc cctgcgtatc
     3541 gttgcccgcc atcactaact gcatagtggt gaagaaatcg cggataaagg ttttcttttg
     3601 attatcgaga ttctgccgga tgccttcgct gatgtgcact gacgaacgct taagttcaat
     3661 cacgcccagc gcaatgccgt taacgtacag caccagatcc gggcgcttgc agtactcgcc
     3721 tttgaccgtc acctcatcgg cgatggcgaa atcattggct tgtgggttgg cccagtcaat
     3781 cagccacacg gtttggttct gctcgcctgc gccttctttg tctttcacgc cgtaacgcag
     3841 taggcgatag acctctttat tggcgtaata gagttttttg ccttcgccca gagcggcggc
     3901 ggaatccagt tgccgcaacg cccgggtgat caacgtatca ttaacgccgc gccgttttag
     3961 ccacgcgctc agtatattag tgtcgatgtt gctgttcccg gtgtacccgg tgcggtgact
     4021 cagatggcgg taacctaaat cttcaacaaa aaactgaatc agacgctgct gtgtggcgcg
     4081 ttcgcgctgg cccacggtac tcaaaaggcg ctccttaaac cctcagcaat ggaactccat
     4141 tacgcggtta atggcaaatt gcagattatt attatcaaac agcctcaact tacggctggc
     4201 ccagcgttcg cccgcaggcc actggtgcag cccttcctta acggaatcca gcgttggttg
     4261 acattcgcca cggctcagca accagtctac ggttgccagc aactccagac caaacggcga
     4321 ttcaaagccg tcaattaact ggctgacctg ctccagcgca ggtagccact ctctggcttc
     4381 gttgttcaga tacgcattaa cgtgttcttt tttctgatcg ttaaaccaga tcacatccag
     4441 cggttggctg tccgggatgc gtttctcggc tttaagatag gtgccgtcca gcgcattaag
     4501 cagatggttc agattgggcg cataggggcc ataatagtga gcttcaaacc gcagtttgag
     4561 gatatcgtcc tgttgatgct gctcaatggc gcgttgtaac agccaggcca gtttctggat
     4621 ttccagtagg ctgcactcca tccccaatac ccagtagcga cgcaccagtt cagcgatggc
     4681 ggctctggcg ggagtgagct tttttacgcc agtgcttttg gcaacgttct ggtatttttc
     4741 agtcggttgg taaatcagga tatcaacgtc ctgaaggtcg ccaagggcag attcaatctg
     4801 cgcccgaacg tccggccagt tcaaaccacc gttgcctgcg cccagtggtg gaatggcgat
     4861 ggactgcacg ttctcttcaa tcaaaaagcg gcgtaagtcc tgcaagccat cttcaatcca
     4921 ctccatgcgg gaatcagcgc gccagtgctg tttggtcgga aaattaacaa tccagcgcgg
     4981 tcccatcagt tccccggttt cggtaatgaa cattttgccg gtgatcacct gcttttgttt
     5041 gcaggcgagg gcatagactt tcatattttc ggggaagcgc tctttaaaca tcaacgcgat
     5101 gcctttcccc atgacaccga cggtattaac cgtattcacc agagcttcca ccggtgcgtc
     5161 cagtaaattg ccttgtgtat atgtgatcat gagaagtacc atccagcccg tgtgtgaacg
     5221 gacattgtta gattacgctg aaacagccat tgttctaatt gtaacctgac ctcttcgctg
     5281 taacaaatta tcccatccag cagagagatc gggcagtgtt gccagattag cgcctctgcc
     5341 tgatagcgtt caaatttagc cggatcgtct ggatcgcggc gaaagtcccg tgcctgcaaa
     5401 atgggccagt cgatctggtc cagactatcc agactggtgt agtagttggt ccagttatag
     5461 taagcatggc tgtcggtaaa cacgaagggt acgtcgtgtg ctgccacgtt tcgcaagttg
     5521 ctgaccagaa taacgatctc ctcattgggg cgttgcttaa tgccgccgcg cccgctgtgg
     5581 atgttcatca gcattggcga gaagggggta aaataaaacg gcacatagtc atgcagtgtc
     5641 ccgcccgttc cgacaggtac cggatgcccg gcacgcttgc cgatcagctc cgggttgccg
     5701 atattgatcc agttttccgc ctgcaccaga ctgttgccgc agtgcagccc gttatccaga
     5761 atccacggga tattgtcccg atggacgata cgccagatca gagctttttg cgggttgagg
     5821 ctggcgctgt aatcataagc catgtgcttt tccttctata ttaagcggat tttcccggtt
     5881 aacagttcct gcatcatccc ttgcttaagc tggcgggttt tgtccagacg ctgttgcagg
     5941 atctggattt ccttgtccat atcggagagg atggcggcga tggcggcttg ttcttctgta
     6001 ttttcaggta atgggatttt aaagcccaac atatctttgt tagttatctg attgatagtg
     6061 gcacccataa cttcagcgat ttgcttttga ataatatcag actggaattg gtgaaatgta
     6121 aatccatata tatcagttcg gaatactgac ataaatgcac cgaatgctga gccctgcgca
     6181 tttttgtcaa ttaatgcgca tttgccaata agttggcgac tgccatttct tacacatacc
     6241 aagatatcac cttctttcac aattgtgcgc tcaggtggtt ctatgctgac gaagacgtta
     6301 tcatcataag ctaagtgatt attttgaatg ttggatgagc gtaataccaa tgtaccataa
     6361 tcagatgtat cttcaggttt gtaagttaac ccaataatgc aattacctag ctcaccaaga
     6421 ttaatacaat tccaatcttc aggaatctcc cccaactcgc tctttttata tcccttagca
     6481 gagccatctt tgcgcaacgc aaactgtggc aaacgggttt taccggtaag tagttgttgc
     6541 atagcagcgg cttttatggc ctgtttttta gcaattaagt tttccagagc gagagtaaat
     6601 gcattaatat catttaatgt tctgacaatt tccgtttgtt caaaggagtt tggtgtaata
     6661 gcaaacttaa gatttttaac ttgagaaaaa ttcagtcctt ctctgtttcc tccggcttga
     6721 taactatcaa tctggttttg tccataattt gaattgatta attcaaccag taaggatggg
     6781 gagagttttt ttgcctctgt tctgatgatg caaacatgtt ggttaacatt accacctata
     6841 accctaaaat cagctttagc gcatcggccg atagatgctc ccgttatgtt gagtaaaaca
     6901 tcatcttgct gaatttcaga accagagaat gtgttatggg tttcatctgt aatatgtacg
     6961 acatcggcga gatctaatct tccccaatca acattttggc tacggacaaa agggcgtccg
     7021 ctatcaatat aaacagatgc tccaccagtt ggtgtttttc cactaccaac tttggttgta
     7081 cagtcatcaa tagagattac tacccaatcc tcaggaatca cccccacctc agtcagctta
     7141 taccccgcag gcacatcttt ccccgccgta cccgaccgtt gctcattcac cattaaatcc
     7201 accatcacca ctccagcccc atcgcccgca aatgcccggc cactttctcg ctcaacgcct
     7261 caacggcggc ttcaagtgct ggcaacggtt cagcataacg ctcttccagc tcctttacgc
     7321 gcgacgccag ttgctgggta atacgctcta tctccgcttt gatccctgcc tgcaacgttg
     7381 ccagccactt atcctgcact accagcgttt taatcgctgc ttcatccagc gtgggataat
     7441 gtttaaatac cgctcgatcc aacgcttcct gtgcggcttt cagcgctttt ttcgcactgg
     7501 cctcggcctt aatcagcgcc agacagcggt tcagcactgc cagctcatcc gggtcggcag
     7561 gggggggctt caactgtgcc agacgggcat tcaccagctt ttgcgtcacc ttatctttat
     7621 cgtttttcac ctcagccagc agatcctctt cccctgcctg ctcctcgatg aattccacta
     7681 gctgacgact ggcttcttca ctgcgctcct gcgcgcggtt cagcgcatcc agcagttccg
     7741 ggaaacagcg aatttccacc agcgaacggg gcagcacatc ggccttgtag cgccgtttgg
     7801 taaattcgaa atcgtgcgtt tctttccaga tgtttttgcc atctttgcct ttcaccggct
     7861 gaagctcgcg gatttgcgcg gcggctttcc agccatcctg cataatcaca tacacatcgt
     7921 cctgcatgac atcagcccag taatccatca ggatctggta cacgctgtat ttgttcagca
     7981 actcactgtt ggcgtactgc gccaatagca tttcgctgat atcgtggatt agcgctttgg
     8041 gtttatctcc tttgcggatc tcttccagac ctgcctcttt tacccacgac ttaaacggta
     8101 gcaggctgcg ggtggcaaaa tccttaaact cctgatgagc cagaatggtc ggtttcacct
     8161 gctgcgcgtc cacttgcgcc cggcagtaac cggggtggtc gtcagcaaac agcacattac
     8221 gcagggcagg gaatacccgc cagtaatcct tcaaagcatc tacatcgcgc actggaatac
     8281 cgccttgtaa gtgcgcggtc agatcgtgca aatcttccgg ctctccactc tcgatataac
     8341 gaggaatatt caggttgtaa tcattcccgg cgatctcgct ggagggcacc atccggctgt
     8401 agcctggcac cgttcgctga tggttgaaca cgtccacaat acggtgaata tcttggctac
     8461 gcaggcggtt cttgttgcca tctttgataa agccccgact ggcgtcgatc ataaaaatgc
     8521 ctttgcggct gtgggcgtgc tctttatcaa tcacaatgat gcacgccggg atgccggtgc
     8581 cgtaaaacag gttggctggc aaaccaataa cgcctttgat atagccctgc ttgattaggt
     8641 tttcacggat attcgcttcg gcgttaccgc gaaacagtac gccgtggggc aggatcaccg
     8701 cgcctttgcc ggtacttttc aggcttttaa tgatatgcaa caaaaaagca taatcgccgt
     8761 ttttttccgg cggaataccc cagccgaagc gttcaaacgg atcttttttc ggcgtcagcc
     8821 cgttggtcca gtttttattg gaaaaaggcg ggttcgccac ggcaaaatcg aaggctttca
     8881 gtttgccgtt cgcctctttc cactgcggat cactcagcgt attgccctgc cagattttcg
     8941 cggtggcatt gttatgcaga atcatgttca tccgcgccag cgcactggta gcgttatcca
     9001 tctcttggcc gaaaatcgac agtccgcgcc gggcttcatc attcactttc aacagcagcg
     9061 aaccagagcc acaggttggg tcataaaccg ttgcatctcg cggcgtatcg ggagtaatac
     9121 caatcacctt tgccagaata cgtgacacct ccgccggagt gtaaaactgt cctttactct
     9181 tgccggattc ggttgcgaag tgacgcatca gatattcata ggcatcgccc agcagatcgt
     9241 cgccttcgac ccggttgctg gagagatcta acccttcaaa gataccgaca agcttgctca
     9301 ggcggtcaat catttccttg cctttgccga gtttatcttc gtcgttaaag tcggcttcgt
     9361 cgatgacgcc cttcaggccg ttctcttccg ccagcttgtg gatgattttg ttgattttat
     9421 cgccgatctc tttgtcattt ttcagcgcaa ccatatcgtc aaagctggct cctttgggta
     9481 tcacgatcat cccgtaagga tcgcctttat acttatcgga aacgtacttc ataaacagca
     9541 gggtcagtac atagtctttg tactggctgg cgtccatccc gccacgcagt tcgtcgcagc
     9601 ttgcccacag ggaagaatag agttcggttt ttttgatagc cattaaatat ctcgttttgc
     9661 gcgataatgt gccgtaagtc ggttacagca gatcggtatt cagaccggtg ccttcgcaaa
     9721 aggcttttgc cgtaatgtgt tccagttcgt cggcatattt aatcatttgc aggtgcagct
     9781 cgacggtgta ctggttacgc ggcgcactgg aaagtgcctg ccggatatga cgataaatct
     9841 cgtcttcatt catattcatg gcaaccactg gtatactgga taagttagct aaattagcta
     9901 aatgtggtat caggttcaag aactacagtg agagtcggat ctgactgcgt gatttatgca
     9961 cgtaaatgct ttgtggcgga ttaatggtcg cagtatcctc tccctccgcg cctgcaaaat
    10021 caggcgtcgg aattagcctc ctgaaaaccg ttacaggaga cttctctata gactacaatc
    10081 cttgtccctg actgcgattt actcgatcta ttgttcgttc ccttataatg ccactataga
    10141 tttcaccgcc ttggtcgaca actgcgcccg ctttgccgaa tccctgatag aaacctagga
    10201 tctcgcgctg tagcttaccc tctgcgggcg atcttgtact gctgcaactc acgcaacacg
    10261 gcctcatccc gccgcatctt aacgaaagcc tgatcagtga tactctcccc accatgccag
    10321 agcaggttat ttctgaacct gattcttgtg cgaatattgc ctcgtgcggg ctgtgcatca
    10381 tcgcagctct ttgataagag gcgtcaaagg taaggggtag cgttattagt ttgggctata
    10441 cacttgcgct acaactcttc aaatagctat atatcaacaa tatttagcca taaaccgtct
    10501 acttccgtct caattatact caaattaagg catgcggcat ctttgtgaca catttgtttt
    10561 tttgatacag gttagagttg acctgctctc cgttgattaa ctcacattgt tgttaaccac
    10621 gtctactttt agcacgacac tgatgcgtta actgagcgaa tggtctgctt tgagcgagga
    10681 gcggacataa caactcacta ggattatgcg cagtttgttt tctaaatata cagttgataa
    10741 gtgctgtaat ccggcactaa cagcaggatg ctaggaagta catttaaatg taaaatgaga
    10801 gtgcactctg tgtgaaaacc actgagtaat cttgagaaca cagtactgaa gcttggtaaa
    10861 aaggagctag tatgtacaac aacctctatg cgctcacaga aagccttttt tgggttgatc
    10921 gacttttaga acaggaatat gaaaatatcg gattgaacga attgctagtt gaattagctg
    10981 aatataataa aaggatagat tcattcatta tcgaacaaac aaattctgta caggagtccc
    11041 ggctgaatgt ttacatggcg gggaatcgta atattgaaag gtataagagt ggtttgtttt
    11101 caatggacag agtcatcctt gatgacgttc tatattcatt gagcctacga tacagggtaa
    11161 atctatcaga aaacgtagtt gaaaaattta ttgaaagcga tagcaaagag aaggttgaac
    11221 atctcaggaa ggaaattgct aggtttatca ggtttgctaa agaaaacttt gatcttatcc
    11281 gtggagagtt tatcgttttt gtacgaagag aatcctctat taaagattcg gagagagcta
    11341 atggaatttt agaagataac tctgatacta atttcctatt tcgatatctc ccccctaata
    11401 ttgcccgtct gtatgaaagt agactatccg tacagaatat agaacgtctg ggcaacacaa
    11461 ataaaattcg gttgttaaac agaaatgttt taacgaatga aattatgttg aaaattaaag
    11521 gctgcgaaag ctcgtataca aatggatata tgtatcaaca tataaataat gccattgata
    11581 atgatgatgg taccatgtct atggaaattg tcatgggaaa gcatagcgga aaaaaaagtt
    11641 atgaacggtg ggtgcaggga gcaaaaaatc gtacagttct ctttcattat aaaaacctta
    11701 ttactgatct taaccaagcg cattcatgca gagcgtccct gggcgcatat tgcccatttc
    11761 atggagaaat tcttcgtaaa ctggatcaaa ggaatgaagt tcagagggtt aaactctcta
    11821 ttgacgttcc atttctagag gggcttactc caagtgaact ctacaaaatt agaactgact
    11881 acgaatcgag ttttaatgcc ttccgctctg tgctacgtga agctgcgtat gacatagaag
    11941 cagaaagtga cccatataag aggcagctga taaatcagca ttttgtcgag cgcatatggg
    12001 atgaaggatt gaccgatatt gagcaaaaaa tacagaccta taagcggaaa agtaataaag
    12061 aactttttct taacattgcg ccatcattga taggttttat tggggcacca aatccagtgt
    12121 cgttggctac aggagccttt acgattctaa aagaggtttt tagtataaca agccactctt
    12181 cggaaataaa gagtcaccct tcatatttcc ttctcatgac gtcaaaaggc aaaaataaat
    12241 tttatgactg aagcagtagg aaatattgtg aaaatatatt cacttaagca aaaaggttga
    12301 ggggctgccg gatctgttat taacggggag ctaattactt tgccgagtaa agcaactcgg
    12361 caaagtagca tagacttgag tgaattatta gattttaact agttcgctag tctctactga
    12421 agctctcaaa ttaatggcag gctcctgtga gccaactcgg tatgtgtaag actgagggtg
    12481 acctctggca atgtcaatga gatcgattag agcctcagtt gctctcagag cttcatttgg
    12541 aagttctcga ggctcgaagc tactgagtgt gccgggcttc atatgtgcaa tctgctcata
    12601 cctaatcttt aatactggtt ctgtgagtgc aaggaacttt tcagctgcca atttcacagc
    12661 ctctggtgaa gcagaatacg agccgcgttt caaaaacatt gatacagatc tcacatcttt
    12721 ccttttatcc gcaagacgcg ctaaacggat caccaacatt tccaacaatg cattgagggc
    12781 taccaactga ttggcaagct catatcttgt actgctatta gtaaggcctt ccatcaaccg
    12841 ctgatacgta tagatggttg ttaggatatg ccatgcttct gcttccattt cttcaagaat
    12901 cgtcataaat actccacaat ggtctgatag ttatcatgca gatatctaaa caaccaattt
    12961 aaactaaatt tataattcaa cacgtagcac atgatactga atcactacgt ataaattaga
    13021 tactttagga ttttatttgg acaaccccgc cccagaagac aattctacct tagtgtatat
    13081 aaggaccgct tatgacatag agcggactgt cagatgaggt ttagttctgt actaatgagc
    13141 cgatacaaat tagctgcata tacctgcatt ccaaataatt tcagacagat attatcgttg
    13201 tgaatccccc gccacctttc cggtgcgcgg ggttttgtct tttttcaccg ggaatacatg
    13261 tatgaatccg tctgatgcca ttgaggcaat tgaaaaaccg ctctcctccc tgccttactc
    13321 actttcccgt cacatcctgg aacatctgcg caaactcacc agtcacgaac ccgtgcctgg
    13381 cattatgggt aaaagcgggg ccggtaaatc ctcactctgt aatgcgctgt ttcaggggga
    13441 ggtcaccccg gtcagtgatg ttcacgccgg cacccgggaa gtgcagcgct tccgtctgaa
    13501 tggccatggt cacagcatgg ttatcactga cctgcccggg gtgggcgaga gccgggacag
    13561 ggatgcagag tatgagaccc tgtaccgtga cattctgcct gaactggacc tggtgctgtg
    13621 gctgattaaa gccgatgacc gggccctgtc tgtggatgag tatttctggc gacacatcct
    13681 gcaccgggga catcagcagg tgctgtttgt ggtgacgcag gccgacaaaa cggagccctg
    13741 ccatgaatgg gatatggccg gcattcagcc ttctcccgca caggcacaga acattcgcga
    13801 aaaaacagat gcggtattcc gtctgttccg gcccgtacat ccggttgttg ccgtatcggc
    13861 ccgcaccggc tgggagctgg atacgctggt cagtgcgctc atgacagcgc ttcccgacca
    13921 tgccgccagt cccctgatga cccgcctgca ggacgagctg tgcacggaat ctgtccgggg
    13981 gcaggcccgt gaacagttta ccggtgcggt ggaccggata tttgacacgg tggagagcgt
    14041 ctgtattgcc tctgttgcac gcacggttct gcgtgccgtc cgtgacacgg tggtctctgt
    14101 tgcccgcgct gtatggaact ggattttctt ctgaacagtc taccagggga aatgcctgat
    14161 tacattgcgt taaccaactg aaaacatgat acaaacacct catgcccctc ctgtttctct
    14221 tgcgtgactg ctctgctgtt aatagaataa aacgatcgat aaaacaggta tcgatcgtta
    14281 ctatcgatcg tttatagtgg ttaatggcct gcgaaaaggc agctgaatct cttcatcatg
    14341 cagaacggaa ttgcacacaa cggactgata cttctctggc ggatgagaga ggggaagcat
    14401 ttatgtccgg gaaaaaccac tttatctgac catgctgttt tgtacctgcc ggtatccaca
    14461 tttgtgggta ccggcttttt tattcaccct ctgtaaggaa aagctgatga aacgacatct
    14521 gaacaccagc tacaggctgg tatggaatca cattacgggc accctggtgg tggcctccga
    14581 actggcccgc tcacggggaa aacgcaccgg tgtggcggtt gcactgtctc ttgcggctgt
    14641 cacgtcagtt ccggcactgg ctgctgacac ggttgtacag gcaggagaaa ccgtgaacgg
    14701 cggaacactg gcaaatcacg acaaccagat tgtcttcggt tcgaccaacg gaatgaccat
    14761 cagtaccggg ctggaactgg ggccggacag tgaagaaaac accggtgggc aatggataca
    14821 gaatggcggg atagccggaa ataccactgt caccacaaat ggtcgtcagg tcgtgctgga
    14881 ggggggaaca gccagtgata cggttattcg tgacggcggg ggacagagcc tgaacgggct
    14941 ggcggtgaac accacgctga ataacagagg cgagcagtgg gttcatgagg gcggggttgc
    15001 caccggtaca attatcaacc gcgacggtta ccagagcgtt aaaagtggcg ggctggcaac
    15061 aggaaccatc atcaacaccg gcgcagaagg cggcccggat tctgaaaatg tatccagcgg
    15121 tcagatggtc gggggaacag cagaatccac caccatcaac aaaaatgggc ggcaggttat
    15181 ctggtcttca ggggtggccc gggataccct catttacgca ggtggtgacc agacggtaca
    15241 cgggcatgcg ctgaatacca cactgaatgg cggttaccag tatgtgcaca aggacggact
    15301 tgcgctgaac acggtaatta acgagggggg ctggcaggtt gttaaggcag gtggtgctgt
    15361 cggtaacacc accataaatc agaacggtga actgagggta catgccggcg gggaagccac
    15421 tgcagtcacc cagaacacgg gcggtgcact ggttaccagt actgctgcaa ctgtcaccgg
    15481 cacaaaccgc ctggggcatt tctccgtggg aaatggcatg gcagataacg tcgttctgga
    15541 aaatggcggt cgcctggatg tactggaagg ccattcagcg cagaataccc tggtggatga
    15601 cggcggtacc ctggcagtgt ctgccggtgg taaggcaaca gatgtcacca tgacatccgg
    15661 tggtgccctg atagcagaca gtggtgccac tgttgagggg accaatgcca gcggtaagtt
    15721 cagtattgat ggcatatccg gtcaggccag cggcctgcta ctggaaaatg gcggcagctt
    15781 tacggttaat gccgggggac aggcaggcaa caccactgtc ggacatcgtg gaacactgac
    15841 gctggctgcc gggggaagtc tgagtggcag aacacagctc agtaaaggcg ccagtatggt
    15901 actgaatggt gatgtggtca gtaccggcga tattgttaac gcaggagaga ttcactttga
    15961 taatcagacg acacaggatg ccgtgctgag ccgtgctgtt gcaaaaggcg actccccggt
    16021 aacgttccat aaactgacca ccaccaacct cactggtcag ggcggcacca tcaatatgcg
    16081 tgttcgcctt gatggcagta atacctcaga ccagctggtg attaatggtg gtcaggcaac
    16141 cggcaaaacc tggcttgcgt tcacaaatgt cggaaacagt aacctcgggg tggcaaccac
    16201 cggacagggt atccgggttg tggatgcaca gaatggtgcc accacagaag aaggtgcgtt
    16261 tgccctgagc cgcccgcttc aggccggcgc ctttaactac accctgaacc gtgacagcga
    16321 tgaagactgg tacctgcgca gtgaaaatgc ttatcgtgct gaagtccccc tgtatgcctc
    16381 catgctgaca caggcaatgg actatgaccg gattctggct ggctcccgca gccatcagac
    16441 cggtgtaaac ggtgaaaata acagcgtccg tctcagcatt cagggcggtc atctcgggca
    16501 cgataacaac ggcggtattg cccgtggggc cacgccggaa agcagcggca gctatggctt
    16561 cgtccgtctg gagggtgacc tgctcagaac agaggttgcc ggtatgtctc tgacgacagg
    16621 ggtgtatggt gctgcaggcc attcttccgt tgatgttaag gatgatgacg gctcccgcgc
    16681 cggcacggtc cgggatgatg ccggcagtct gggcggatac ctgaatctgg tacacacatc
    16741 ctccggcctg tgggctgaca ttgtggcaca gggaacccgc cacagcatga aagcatcatc
    16801 ggacaataac gacttccgcg cccggggctg gggctggctg ggctcactgg aaaccggtct
    16861 gcccttcagt atcactgaca atctgatgct ggagccacaa ctgcagtaca cctggcaggg
    16921 actctccctg gatgacggcc aggataacgc cggttatgtg aagttcgggc atggcagtgc
    16981 acaacatgtg cgtgccggtt tccgtctggg cagccacaac gatatgacct ttggtgaagg
    17041 cacctcatcc cgtgacaccc tgcgcgacag tgcaaaacac agtgtgcgtg aactgccggt
    17101 gaactggtgg gtacagcctt ctgctatccg caccttcagc tcccggggtg acatgagcat
    17161 ggggacagcc gcagccggca gtaacatgac gttctcaccg tcccggaatg gcacgtcact
    17221 ggacctgcag gccggactgg aagcccgtat ccgggaaaat atcaccctgg gcgttcaggc
    17281 cggttatgcc cacagcgtca gcggcagcag cgctgaaggc tataacggtc aggctacgct
    17341 gaatatgact ttctgattat acggtatccc ggaaatacga tttcaggaaa attcatgcag
    17401 taatgggaaa ggacgctcat tgctgttcac cgcagttctg aattattgaa tttataaagg
    17461 tgaatgatgc acgaaaagaa catcgccctg ctttgtgatg aagccgaccg gcttttgcaa
    17521 ctgaatatca atctgctccg gcaaatggtt gatgagccgg atgtgttatc tgacagtaag
    17581 aacgaaaacg ggctgctttt tgataaacgg aaagcgctga aaagaattga ggagctggag
    17641 ggcgaacaaa tcaaaaccgc ccgcagggag atggtgctgg ctgtggtcgg cacgatgaag
    17701 gcgggcaaat caaccaccat caacgccatt gtggggcagg aaattctgcc caaccgtaac
    17761 cgccccatga cctcggtacc gacattgatc cgccacgttc ccggaaaaac ggaaccggtc
    17821 ctccatctgg aacatattca gcccgtccgc aatttattaa tcacactgca ggaaaaactc
    17881 gccaccccgg cagggcagca agtcgcacag tccctgcagc aaaccgggga tacccgcgaa
    17941 ctactggata ttctggcgga tgatgtctgg cttaaaaatg aataccacgg ggaagacgaa
    18001 atctttaccg gactggcatc gttaaatgac ctggttcgtc ttgctgcggc aatggggact
    18061 gaatttcctt ttgacgaata cgcagaagtg cagaaactac cggtgatcga cgtggcgttc
    18121 agccacctgg tgggaatgga tgcatgtcag ggaacactca cactgctgga tacccccggc
    18181 cctaatgaag ccggacaacc acagatggaa atgatgatgc gggatcaact acagaaagcc
    18241 tctgcggtgc tggcggtgat ggattacacc cagatgaact cgaaagccga tgaggaggtt
    18301 cgtaaagagc ttaatgccat tgctgacgtc tcagccggtc gtctgtttgt gctggtcaat
    18361 aaattcgatg aaaaagatcg caatggtgat ggtgcggatg ccgtacgcca gaaggtgcca
    18421 gcaatgctga acagcgatgt gctgcccgcc tcccgcgttt atcccggctc atcacgtcag
    18481 gcgtacctgg ctaaccgtgc gcttcatgag ttacgaaaaa acggtgcgct tccggttgat
    18541 gaagcctggg tcgatgattt tatcagggaa gctttcggac ctatggtgga agaggacgac
    18601 tggaaagaca gcactaaggt aaataaaaag gcagaaaaac tctggaatat atctctgatc
    18661 gatcaactga taacggaagt catacagagc tcccattcca gagcggcggc actggcggtt
    18721 gactctgccg ccgcaaaact gatgcagaat gcagaaaatg tcagcgaata tctgtcatta
    18781 cgtcatcagg ggctgcagca aagcattcag tcactacagg cgcatattac cagcctgctg
    18841 gcggatatcc aggagattga ggaatgtcag aatcaggtga ccggtgatgt cagaatggcc
    18901 atggaggaca tcaataccaa aaccggggaa ttactgacga aggtctgtgc ctcactggag
    18961 gaggagctga acgactattt cagaagcggt aaacgcaaag aacagcaaat gctggaggaa
    19021 gaaaattcag cgcagccccg ggagagaaac gcattcgctt tctttcacga tattttcgga
    19081 accggaaatc agcatgacag gatgcgggac tttgatccgg acagccccga aataaaattc
    19141 agtgatcgca ggg
//
LOCUS       DAZFAB010000088        19010 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZFAB010000088 DAZFAB010000000
VERSION     DAZFAB010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19010
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.088"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(66..431)
                     /gene="yhaH"
                     /locus_tag="ACHJWA_003483"
     CDS             complement(66..431)
                     /gene="yhaH"
                     /locus_tag="ACHJWA_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO4310013.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            complement(723..1709)
                     /gene="yqjG"
                     /locus_tag="ACHJWA_003484"
     CDS             complement(723..1709)
                     /gene="yqjG"
                     /locus_tag="ACHJWA_003484"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFO4310014.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVEKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTRIISIGPWQDLDEPHGRDVRLG"
     gene            complement(1779..2171)
                     /gene="yqjF"
                     /locus_tag="ACHJWA_003485"
     CDS             complement(1779..2171)
                     /gene="yqjF"
                     /locus_tag="ACHJWA_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO4310015.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITGYAGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            complement(2357..2656)
                     /gene="yqjK"
                     /locus_tag="ACHJWA_003486"
     CDS             complement(2357..2656)
                     /gene="yqjK"
                     /locus_tag="ACHJWA_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFO4310016.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            complement(2646..3050)
                     /gene="yqjE"
                     /locus_tag="ACHJWA_003487"
     CDS             complement(2646..3050)
                     /gene="yqjE"
                     /locus_tag="ACHJWA_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO4310017.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            complement(3053..3358)
                     /gene="yqjD"
                     /locus_tag="ACHJWA_003488"
     CDS             complement(3053..3358)
                     /gene="yqjD"
                     /locus_tag="ACHJWA_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFO4310018.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            complement(3396..3764)
                     /gene="yqjC"
                     /locus_tag="ACHJWA_003489"
     CDS             complement(3396..3764)
                     /gene="yqjC"
                     /locus_tag="ACHJWA_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFO4310019.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHQKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            complement(3911..4294)
                     /gene="mzrA"
                     /locus_tag="ACHJWA_003490"
     CDS             complement(3911..4294)
                     /gene="mzrA"
                     /locus_tag="ACHJWA_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFO4310020.1"
                     /translation="MQIPRMSLRQLAWSGAVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPQGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            complement(4298..4960)
                     /gene="yqjA"
                     /locus_tag="ACHJWA_003491"
     CDS             complement(4298..4960)
                     /gene="yqjA"
                     /locus_tag="ACHJWA_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFO4310021.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            complement(4982..5065)
                     /gene="yqiM"
                     /locus_tag="ACHJWA_003492"
     CDS             complement(4982..5065)
                     /gene="yqiM"
                     /locus_tag="ACHJWA_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFO4310022.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            complement(5305..6081)
                     /gene="exuR"
                     /locus_tag="ACHJWA_003493"
     CDS             complement(5305..6081)
                     /gene="exuR"
                     /locus_tag="ACHJWA_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFO4310023.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            complement(6211..7509)
                     /gene="exuT"
                     /locus_tag="ACHJWA_003494"
     CDS             complement(6211..7509)
                     /gene="exuT"
                     /locus_tag="ACHJWA_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFO4310024.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            7992..9404
                     /gene="uxaC"
                     /locus_tag="ACHJWA_003495"
     CDS             7992..9404
                     /gene="uxaC"
                     /locus_tag="ACHJWA_003495"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFO4310025.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMSGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            9419..10906
                     /gene="uxaA"
                     /locus_tag="ACHJWA_003496"
     CDS             9419..10906
                     /gene="uxaA"
                     /locus_tag="ACHJWA_003496"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFO4310026.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVRLRQDVARGHKF
                     ALTNIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            10989..11540
                     /gene="ygjV"
                     /locus_tag="ACHJWA_003497"
     CDS             10989..11540
                     /gene="ygjV"
                     /locus_tag="ACHJWA_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFO4310027.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(11545..12789)
                     /gene="sstT"
                     /locus_tag="ACHJWA_003498"
     CDS             complement(11545..12789)
                     /gene="sstT"
                     /locus_tag="ACHJWA_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFO4310028.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(13188..14153)
                     /gene="alx"
                     /locus_tag="ACHJWA_003499"
     CDS             complement(13188..14153)
                     /gene="alx"
                     /locus_tag="ACHJWA_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFO4310029.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRVG"
     gene            complement(14437..15423)
                     /gene="ygjR"
                     /locus_tag="ACHJWA_003500"
     CDS             complement(14437..15423)
                     /gene="ygjR"
                     /locus_tag="ACHJWA_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO4310030.1"
                     /translation="MIRFAVIGTNWITHQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     EFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(15502..16194)
                     /gene="ygjQ"
                     /locus_tag="ACHJWA_003501"
     CDS             complement(15502..16194)
                     /gene="ygjQ"
                     /locus_tag="ACHJWA_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFO4310031.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWSDVNVVPARNVGLLLGARPGNRYFTRRIDTAAELYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRANKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLEKGRGKIVRLREKLARVSAVIDAKIFNRQPKYLGPSVII
                     GPFSEHGCPAQK"
     gene            complement(16271..16774)
                     /gene="ygjP"
                     /locus_tag="ACHJWA_003502"
     CDS             complement(16271..16774)
                     /gene="ygjP"
                     /locus_tag="ACHJWA_003502"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFO4310032.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            16859..17995
                     /gene="rlmG"
                     /locus_tag="ACHJWA_003503"
     CDS             16859..17995
                     /gene="rlmG"
                     /locus_tag="ACHJWA_003503"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFO4310033.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTPQTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            18279..18593
                     /gene="higB"
                     /locus_tag="ACHJWA_003504"
     CDS             18279..18593
                     /gene="higB"
                     /locus_tag="ACHJWA_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HFO4310034.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            18590..19006
                     /gene="higA"
                     /locus_tag="ACHJWA_003505"
     CDS             18590..19006
                     /gene="higA"
                     /locus_tag="ACHJWA_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFO4310035.1"
                     /translation="MIAIADILHAGEKLTAVAPFLAGIQNEEQYIQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
BASE COUNT         4867 a         4919 c         4817 g         4407 t
ORIGIN      
        1 attattcatt catcaacatg atgataaaag ccggatgagt cagacatccg gcgagcagat
       61 ttgttttaag gttccagttt tggatcaggt ccaaagcggt tttcgcctgg cgtacccgcc
      121 tggcagttaa agacgatgat aataagccag ccgataaacg ggattaagaa tagcagtgcc
      181 caccacgccg agcggtcggt gtcgtgcagg cggcggaact gaactgccca ccacggtaaa
      241 aacaccagaa taccgtagat tgtcgtcagg atgccttcgc caccagcgcg ttgccagcct
      301 aacattttat ccagcagccc cagcacgaac gtaaagatga tgttgaccag aataaacatc
      361 cagtactctt tgcgccgcgc acgaccccgg aaaccgacat aattttttag tactttcaga
      421 taccagtcca tttttgcctc aattttgctc aatcacttgt ttttaaagca gcaaattaag
      481 aatagacgtg aatgaaatgg cagtaaatat tcgttcaagg aattaataaa atgctgtgaa
      541 ggcgcgctaa gactgatgac aagcgcaaaa ttgccagatg cgctacgctt atctggcccg
      601 cgcggtcatt gcaacatatt gaatataaaa gttttgtagg tttgataagg cgttcatgcc
      661 gcatccgaca tgaacaacat gcacttagct aattatcaga ggcacctcgt aaggtgcttt
      721 ttttaaccta agcgaacatc tcgtccatgc ggttcatcga gatcctgcca cggaccaatt
      781 gaaataatcc gcgtagggtt gatggtctta tgactgcgga agtaatgatt acggatatga
      841 tcgaaattga ctgtttcggc aattcccggc atctggtaga tatcacgcag gaagccatac
      901 agattaaggt aatcgctgat gcggtgctta tcacacttga agtgggtcac atacactgga
      961 tcaaaacgga ccagcgtggt ccacaggcga atatcggctt cggttagctg gttgccggtc
     1021 aggtaacgat gctgacctaa aatctgttcc agtcgcgcca gcgattcaaa cactttctcc
     1081 accgcttcgt cgtaggcttg ctggctggtg gcaaaaccag ctttatacac gccgttgtta
     1141 acggtgtcat aaatccagcc gttaagttcg tcaattttcg tttgcagggc tggtgggtag
     1201 taatcacccg ctttcgcgcc cagcgcatca aacgcggtat taaacatgcg gatgatttct
     1261 gctgattcgt tgctgacgat ggtgtggttc tttttgtccc acagcacggg aacagtaact
     1321 cgtccgctgt agtgtggatc ggcgtggaga taaagctgat acaaaaattc atgttgatag
     1381 agcgtgtctc cggttgctcc cggaaaactg tcatcaaagg tccagccgtt ttccagcatc
     1441 agcgggttca ctacggaaac ggaaataaac ggttccagtc ctttgagttt gcgcatgatc
     1501 agcgtgcggt gcgcccacgg gcaggcgagt gaaacataga gatgataacg atctttctct
     1561 gcgataaaac cgcctgtgcc agtggggcca ggagcgccat cggcagtgag ccagttacga
     1621 aatgcggaag ctgaacgttg aaatttaccg ccggtagatt tggtatcgta ccaggtgtca
     1681 tgccagacgc cgtcaatcag ttgacccatt gttctctcct ccaatagcaa aaacgaggag
     1741 cttcctcctc gttttttatc gttaagtata gcgcgtgctt accacttttt attcagcagg
     1801 cggtcgatgc tatacgcgcc cggaccggta attgccagca gcaggaatcc gccagaaatt
     1861 gtcaggtttt tcatgaacat cagcgagttg acgccttcag caaagttgct gtgaaataaa
     1921 aatgccgtca gcagcgtaaa gcccgcagta aacagggctg tggtgcgagt caggaaaccg
     1981 aacaggattg ccagaccacc accaaactca agcagaatca ccagcggcag cataaaaccc
     2041 gggacgccca tcgcttccat atattgttgg gtacccgcgt aaccagtaat ttttccccag
     2101 cctgcggtaa taaacagaat cggcattaaa atgcgcgcta ccagtacacc aacatcttct
     2161 aattttttca tcatactctc cactgcgcca ctcaggctgc tgattgtttt tttgtgcaag
     2221 ggcgcggtat tagcgtcgtt gctgtcgatg gagagaatca taaacgtggt gaatgatgat
     2281 tgttagcaag gaaaactgtc aaaaatcttc aaaaaatttg atgggtaagg ccggaatggc
     2341 tccggccaga gggaagttaa ccgcgaagct gttgctgctt gagggtcgtt ttaaccagac
     2401 gccaggcgct ccatacgcca aaaccgcgtc tggcccagcg gaccagcata ttaggatggc
     2461 gaatcgtcca gatcgccatc acgctactgc caaccagcgc ccaggagcgc agacttagca
     2521 gcatattcca gcgacgatcg taagcgcctg ttgcctccag ccattcacga cgactggcag
     2581 aaagatccag ccgttgttgc tggatctggc taagcagttg cgccttacgt cgttcacgtt
     2641 cgactttact gctcacggga ctcctcctcg agcagctgcc gatcgtttgc cagctcatgg
     2701 cgtgtatggc gcagcaaggt ggatttacgc gatttacgta acgtccagat accgccaatc
     2761 agtgccagta gcagcaacac cacggtggtg gcaatcatcg cattcaggcg atattgcgga
     2821 tcaaccgccc aaataattag caccatcagg ctcataagac caaatgcagc gaaaagcatc
     2881 gtcaggccca gcatcagtaa aagttgaaag agattagctt tttcctcttc cagctcaacc
     2941 accgccagcc gcagacgtgt ttccaccatt tcaaccatga tggaaacaat tcgctgcccg
     3001 atgcccagaa cgcttttacc gggcccttgc gcgtgatgag tgtccgccat aattaacgac
     3061 gcgacagcag aacgccgagc actacaccga ttgcagcgcc aatgcccacg cccgtccatg
     3121 gattttcacg cacatactca tcggcacgcg ccgccgcgac acgggtttgt ttggcgatgg
     3181 catcaccggt ttcacccagg cgataacggc tctgtttcag tgcctgctcc gctttgctac
     3241 gaatcttact caactcttct ttcgacttct cgccagatga gctaagcacc tcttccagcg
     3301 tatcggaaag ggatttcaac tcagcacgca gatgttccgt agtgtgttct ttcgacatag
     3361 ttttctccag gtgagtaagt agtgactatt gtgagttagt agtcgcgcgc ttccagcttt
     3421 ttcagctctt cctgcgcttc tgccagtttc cgttcgcgtt tggcaatctt atcggcatcg
     3481 cctttttgct tcgcctcggc taaatcttgc tggcgttccg ccacctcatc tttctgcttt
     3541 gcgattttct tctgatgatc ggcacgcagc tggctatctg aacagttggc ccggacttca
     3601 ctcagggctt tattcagacc gtcaatacga ttctggtttt ggtgtttttc ggcatagctg
     3661 atctccttaa ggatattttg ctccttttcc tgacacaggg tagtggcata actaccggca
     3721 ctaagagcaa aaagagaaac agctaaagcg atgcggtatt tcattcttga accttccatt
     3781 gtgattcgga cctattacaa aggaacgatt gtccagacaa cgaagcggta gtgttaacca
     3841 ccgcctcatt aatcatagac agttacggtg aaatcaccaa agtgagtgat ctgtttcgga
     3901 caacaggaag ttatccgaag cgatgagagt tatcccgtag ccgggtcagc cactgcatag
     3961 cctgactgtt attgtcctgc tgcgcaatga tgtagccttg gggcaatgtt ctgtcgagga
     4021 ccgcttttgc agcggcgctc tggtcgctgg agtcaaaggt aattaacaga gtgtcgtttt
     4081 tgggggtgat acttttgaaa ggaatgccat gagcgtcaag gtgatgccag attgaaaaac
     4141 cgtctggcat cgttgtgcct tgatgaacgg cacgaatcgc cagcgtagac tcttgctggc
     4201 gaaccgcgga ccaggccagc aacagcgtgc cgaccagaag taaaacagcg ccggaccagg
     4261 ctagctggcg aagcgacatg cgaggtattt gcatccctta cccccgattt ccatatttct
     4321 ttttccataa tacgaccaga gaacctgcca ggccaaacac cagcagcacc accgggagca
     4381 gcatcaggca tgacatcagc tggtcctcgt actttaaaaa taccggcgtt ttgccgagca
     4441 tgtaacccag agttgtcagg atcaataccc acagcagacc gctcatccag ttgaaaaact
     4501 gaaagcgcgc gttattcagc cctgataacc cggcaatcgt cggcagcagt gttctgacaa
     4561 acgcaataaa gcgaccaatt aacagcgccg ataaaccgtg tttatgaaaa agatggtgtg
     4621 cgcgttgatg ataatgcgcg ggtaaatgag atagccagtt ttgtacggtg cgggtattgc
     4681 ccagccatcg cccctgaata tagctgaccc agcagccgag gctggcggca acggtcagca
     4741 gcagaatcgt ttgcggatag cccatcgcgc ctttcgcaat caacacgccg accaatacca
     4801 gtaaactgtc gcccggtaaa aaggccgccg gaagcaagcc gttttcaagg aacaaaatta
     4861 caaacaagac aaaatacaac atgccaatca tcgatggatt ggccagggtt tcaaaatcct
     4921 gcgcccacag ggcttgcagc aattgggtca aaagttccat tcattattcc tggtgaatcg
     4981 gttaacgcca cgctggtttt aaaagatgca ctaccgcaat gttattgctg ttttggtatg
     5041 ttctgcgggt ttgatacatc aacacagcct gaactattcc ctgttaaatc gtgagataag
     5101 aaagcattca aaaacataaa ttcaaattga atctaattgt aacaaaggtc aacgttgttc
     5161 gttatcaaaa ttacatgtcc gtggagtctg attttgctga atgcagggga gggtggcgag
     5221 aggatttaca caggctgaca ctttatactt ttcgcttacc gacgagacaa acaggcgcgt
     5281 cgcagcgcct gtcggacagg tatttcattt actgccactg gtggccgtat cgagatgcac
     5341 taccgggttt tcggcgaaca gatagcggtc ggcattgaac tcgaagtcgt cgctggtttc
     5401 gttgaataac atgatcttgg tgttttccag gtgctgccac attgccagct tagcagcatg
     5461 aggatctttg cgaatcagcg ccttgaggat ttgatcgtgg tcatcacacc agttatcgac
     5521 ggtacgggaa tcaatgtgtt cgtgcagttt tttccagtac gggttatggc tacgctgggt
     5581 ccacattttt tcaacgatag ccgccagggc ggagttctgc gtcgccaggg ctacctgaat
     5641 atggaactgc aaatcccact cggaatcacg gaagcattgt tcgccgcgcg cctgctcctg
     5701 gatggccatc aatttcatga tgtcctgttt cgttacctga gttgctgcga actcggcaat
     5761 attactttcg atgagttggc gagcctggag taactcaaac ggaccgtaat tggcgaattc
     5821 catattattg tccgcagcct gctgatggcg cggctggttg gaaaccacgt gaatacctga
     5881 acctttacgc acttcgacat agccttcaac ttccagcatg atgatggctt cacgaactac
     5941 cgtacggctg acgttctttt catcggcaat aaagcgctca gcaggcagtt tatcacccac
     6001 cagatagacg ccctgttcga tgcgctcttt caggtcagcg gcaagttgtt gatacaaacg
     6061 gcgtggttca gtgatttcca tatgcgctcc aggcatgata cggcagacta tatttgttat
     6121 accacttttg cgagtttagc tctactcgca gtgatgaaaa agccgcctga gaaggcggct
     6181 ttgaagcgaa actaaaactt acacgaagcg ttaatgttgc ggtgcgggat cgttatgtgt
     6241 ttcctgcgcc acctcaatcg ccggtttgtt ctgcaatacg gtccagataa ccagcgcacc
     6301 cagcaggtca aatactgcca gaactgcgaa cagcgggctg aagccgatgg tgtcagccag
     6361 tgcaccgaca accagcgcaa acagcgtact tgccagccat gcggacatcc cggttaaacc
     6421 gttcgccgtt gccacttcgt tacgaccaaa cacgtcggaa gagagcgtaa tcagcgcgcc
     6481 agacagtgcc tggtgggcaa aaccaccgat acacagcagc ataattgcga catacgggtt
     6541 ggtgaacagg ccgatcatac ccgggccaat catcagcact gcacccagcg ttacgaccat
     6601 cttacgggaa acgatcaggt tcacaccaaa ccaacgctgg aacagcggcg gcaggtaacc
     6661 accgaggata caaccgaggt cagcaaacag catcggcatc caggcgaaca tcgcgatctc
     6721 tttcaggtta aagccgtaaa ctttaaacat gaacagcggg atccacgcgt taaaagtacc
     6781 ccaggccggt tctgccagga aacgcggcag cgcgataccc cagaactgac ggttacgcag
     6841 gatctgacca acggacattt tcttcgccgt actcacctgg tgctgggctt cctgaccatt
     6901 aataatatag tcgcgttctt catcggtcag atgcttctgg tcgcgcggat gtttatagaa
     6961 aatcagccat gccatcgccc agataaagct caatgcaccg gagatgataa atgccatctg
     7021 ccagctgtgc atcacgattg cccataccac cagcggcggc gcaatcatcg caccaatcga
     7081 agaacctacg ttaaaatagc ctactgcgat ggaacgctct ttcgccggga accattcgga
     7141 gctggctttc agacccgccg ggatcatcgc tgcttccgcg gcaccgaccg caccacgagc
     7201 aacagccagg ccaccccagc tacctgccag cgcggttgca ccacagaaca cggcccacag
     7261 cacagcaaac attgcataac cgattttcgt acccagcaca tccagtacat aacctgctac
     7321 cggttgcatg accgtataag cagcagaata ggctgcgatg atataggaat actgttgggt
     7381 ggagatgttt aactcttcca tcagagttgg cgcagctgcc gccacagtgt tacgcgtcag
     7441 gtaaccaagc acggtgccga gcgtcaccag tgcgatcata taccaacgta accctttaat
     7501 tttacgcatg taaaacctca tcgttttgtt atatccgcag gaagcgcggc catcatcccg
     7561 ccagaggctg aagatgttag ttacgaccgg gtaaccgaaa aaaaaccggc acgccccgaa
     7621 cgttgccatg aaagtttcgt gctaattcgg cttccgtacc ggttaacccg attcatctaa
     7681 caaatgaatg aatcggcaat ttgcattccg tcgacttatg atttgcgacg gcagaaagat
     7741 aacttgtcat acaactttaa aaggtgagag ccatcacaaa tgtgcgaata tttgtaggga
     7801 cattacctga cgacagcgag gccagtactg gcgcggcctg cagagagatt taccactttg
     7861 agagtaattt ttttaactac gtttattgat ctaactcacg aaaatatctt cggactctgg
     7921 aaattggtgt gataactttg tcagcatcgc accataagca agctagctca ctcgttgaga
     7981 ggaagacgaa aatgactccg tttatgactg aagatttcct gttagatacc gaatttgccc
     8041 gccgtctgta tcacgactac gcaaaagacc agccgatttt cgattaccat tgccatttgc
     8101 cgccgcagca gattgcggaa gactatcgtt ttaaaaacct gtatgacatc tggctgaaag
     8161 gcgatcacta caaatggcgc gctatgcgta ccaacggtgt ggccgagcgt ctgtgtaccg
     8221 gtgatgcgtc tgaccgtgaa aaatttgacg cctgggcggc gactgttccg cacactatcg
     8281 gcaacccgtt ataccactgg acgcacctcg aactgcgtcg tccgtttggt atcactggca
     8341 aattgctttc tccgtcaact gccgatgaaa tctggaacga atgtaacgaa ctgctggcgc
     8401 aggataactt ctccgcgcgc ggcatcatgc agcagatgaa cgtgaaaatg gtcggcacca
     8461 ccgatgaccc gatcgattct ctggagcatc acgcagagat cgccaaagac ggctctttca
     8521 ccatcaaagt gctgccgagc tggcgtccgg acaaagcctt caacatcgaa caggcgacct
     8581 ttaacgacta catggcgaag ctgggcgaag tttccgatac cgacattcgc cgctttgctg
     8641 acctgcaaac tgccttgacc aaacgtctgg atcacttcgc cgctcacggc tgtaaagtgt
     8701 ctgaccatgc gctggacgtg gtgatgtttg ctgaagcgaa cgaagcggaa ttggacagca
     8761 ttctggcgcg ccgtctggct ggcgaaaccc tgagcgagca cgaagtggca cagttcaaaa
     8821 ctgcggtact ggtgttcctt ggtgccgaat atgcacgtcg cggctgggta cagcagtacc
     8881 acattggcgc gctgcgtaat aacaacctgc gtcagttcaa actgctgggg ccggatgtag
     8941 gctttgactc catcaacgac cgtccgatgg cagaagagtt gtctaagctg ctgagcaagc
     9001 agaacgaaga aaacctgctg ccgaaaacca ttctgtactg cctgaacccg cgcgataacg
     9061 aagtgctggg caccatgagc ggtaacttcc agggcgaagg tatgccgggc aaaatgcagt
     9121 tcggttccgg ctggtggttt aacgatcaga aagacggtat ggaacgtcag atgacccaac
     9181 tggcgcagct cggtttgctg agccgctttg tcggtatgct gactgacagc cgtagcttcc
     9241 tgtcatacac ccgtcacgaa tacttccgcc gcattctgtg ccagatgatc ggtcgctggg
     9301 tggaagcggg cgaagcaccg gcggacatca acctgctggg cgagatggtg aaaaatattt
     9361 gctttaacaa tgcgcgtgac tacttcgcca ttgaactgaa ctaaggtctg gggttgatat
     9421 gcaatacatc aagatccatg cgctggataa cgtcgcggtc gctttagcgg atttggctga
     9481 aggcacagaa gtcagtgtcg ataaccaaac tgttaggctg cgccaggatg ttgctcgtgg
     9541 acataaattt gcgttaacga atatcgcaaa aggggccaac gtcatcaaat atggcctgcc
     9601 gattggttat gcattggcgg atattgcggc gggggaacac gttcacgccc acaatacgcg
     9661 cacgaatctg agcgatctgg atcagtatcg ctatcaacct gattttcagg atctgcctgc
     9721 gcaagcggca gatcgtgaag tgcagatcta tcgtcgcgct aacggcgatg tcggggtgcg
     9781 taacgagctg tggatcctgc caaccgtggg ctgtgtcaac ggcatcgcgc ggcagatcca
     9841 gaaccgtttt ctgaaagaga ccaacaacgc tgaaggtacc gacggcgtgt tcctcttcag
     9901 ccacacctac ggctgctcac agctgggcga cgatcacatt aatacccgca ccatgctgca
     9961 aaacatggtg cgccacccga acgcaggcgc agtgctggtg attggtctgg gctgtgaaaa
    10021 caaccaggtt gccgcattcc gtgaaacgct gggcgatatc gatcctgaac gcgttcattt
    10081 catgatctgc caacagcagg atgatgagat cgaagccgga atcgagcatt tgcatcagct
    10141 gtataacgtg atgcgcaacg ataaacgcga gccaggcaaa ctcagcgaac tgaagtttgg
    10201 tctggagtgc ggtggttctg acggtctttc tggtattact gctaacccga tgctggggcg
    10261 tttctctgac tacgtgattg ctaacggcgg tactaccgta ctgaccgaag tgccggagat
    10321 gtttggcgca gagcagttgc tgatggacca ttgccgcgac gaagcaacgt ttgaaaaact
    10381 ggtcaccatg gtcaacgact tcaaacagta ctttattgcc catgaccagc cgatctatga
    10441 aaacccatcg ccggggaaca aagcgggcgg tatcaccacg ctggaagata aatcccttgg
    10501 ctgtacccag aaagcgggtt ccagcgtcgt ggttgacgtg ctgcgttacg gcgagcgtct
    10561 gaaaacgccg gggctgaact tgttaagtgc gccgggtaac gatgccgtag cgaccagcgc
    10621 cctggcgggt gcgggctgcc atatggtgct gttcagtact ggtcgtggca cgccatacgg
    10681 cggctttgta ccgacggtga aaatcgctac caacagtgaa ctggcggcga agaaaaaaca
    10741 ctggatcgac tttgacgcgg gtcagctgat ccacggtaaa gcgatgccgc agttgctgga
    10801 agaatttatc gataccatcg ttgagtttgc caacggtaaa caaacctgca acgagcgtaa
    10861 cgacttccgc gaactggcga tcttcaaaag cggcgtaacg ctataaagtc gaaatgagtt
    10921 gttaataaaa aacggcgttt cataatgtga agcgccgttt gcttttccat acctgataag
    10981 gatttatcat gaccgcgtat tggctggccc agggcgtggg tgtcatcgcc tttctgattg
    11041 gtatcacaac atttttcaat cgtgacgaac gtcgcttcaa aaagcagctt tcggtctata
    11101 gcgccgttat tggcgtacat ttttttcttc tgggcaccta tcccgctggt gccagtgcca
    11161 tccttaatgc cattcgtaca ttgattacct tacgcacgcg cagcttatgg gtaatggcga
    11221 tttttattgt gctgactggc ggaattggcc tcgcgaagtt ccatcatcct gtcgaactat
    11281 tgccggttat cggtacgatt gtcagtacct gggcgctgtt ccgctgtaaa gggctcacca
    11341 tgcgctgcgt gatgtggttt tcaacgtgtt gctgggtgat tcacaacttc tgggcggggt
    11401 cgataggcgg cacgatgatt gagggtagtt ttctgcttat gaatggcctg aatatcattc
    11461 gtttctggcg gatgcagaaa aggggaattg atccgtttaa agtagagaaa acccctcccg
    11521 ccatagacga aaggggttaa acaattaatt acgcagggcg ctatttgcca gacgatcgtc
    11581 ttccgcctgg caagctgccg cagtgaacag cacgtcagtt gaagagttca gcgcggtttc
    11641 gcaagagtcc tgcaatacgc cgataataaa gccgacggca accacctgca tggcgatatc
    11701 gttcgaaata ccgaacatat tacaggccag tgggatcagc agcaaagaac cccccgccac
    11761 gccggatgcg ccacaggcac acagagaagc cactacgctc aacagcagcg ctgtgggcag
    11821 atcgaccgga atacccagcg tattaaccgc agccagcgtc aacacggtaa tggtgattgc
    11881 tgcgcccgcc atattgatgg tggctcccag cggaatagaa acggaatagg tatcgcgatc
    11941 cagattcagc ttttcacaca gtgccatatt caccggaatg ttcgccgcag agctgcgggt
    12001 gaagaaggca tacacgccgc tttcgcgcag gcacagcagc accagcggga acgggttacg
    12061 acgaattttc caccacacca gcaatgggtt aaccaccagc gccaccagta acatacagcc
    12121 aaccagcacg accagcagtt gcgcgtagcc ccacagtgtg gagaaaccgg tggttgccag
    12181 ggtagaagaa accagaccaa aaataccaat cggtgcgaag cgaatgacca gtttcaccat
    12241 aaaggtgacg gcattcgaca tatcgttgac caggtttttg gtggtctcgt taccgtgacg
    12301 cagtgcgaaa ccgaggccaa ttgcccacac caggatcccg atgtagttac ctttcagcag
    12361 cgcgtcgatg gggttggaaa ccatgctcat taccagcccg cgcatcactt ctacaatgcc
    12421 tgacggtggc gaaatatcac ccgcgctact ggataagtgc agggtagaag ggaaggcgaa
    12481 gctgaaaacg actgcggcca gagcagcaga gaaggtaccc agcagataga ggaacaaaat
    12541 agggcggata ttggttttct gcccgtgctg gtggttagca atagatgcca tcaccagcat
    12601 caacaccagg atgggggcaa cggctttcag tgcgccgacg aacaaagtac ctaacagacc
    12661 aacagcttcc gccgcgggtt ttgagatcca tgccagaaga atccccagaa caaggccgac
    12721 caggatttgt tttaccaggc tgccatgagc cagacgccgg aatagccccg gtgaacgttg
    12781 cgtagtcatt tttcgatcct ttcattgtgt tgtcgcacat ccctggtgcg ttctgtcgac
    12841 gcatctttca ggatgtaaca aagtgtttgc aaggtcgagt ataaggaaag ttacagcttc
    12901 gggaagcgaa aaatgctgga ttttaaagag aggtcatatt ttgcaactta atggttacat
    12961 ttatttgaca taaaaataaa gccaacgtgc ggcttccaga ctgaggacta cgtcaaaaaa
    13021 ggactcggtc agtcccctcg ccccgtttgg ggagagggtt agggtgaggg gaacaggcca
    13081 gcatcggtgc gaaaaattac cctcaccccg accctctccc tggaagggag agggagaaaa
    13141 cggtgctcat gttgatgaca gggtacacgg cttaggcaga ttaaaaatca tccaacccgc
    13201 tgcttatcat gccgataatt cacccaggcg ttgataataa acgtcatcac caggatgcca
    13261 aacaccacgc ccagcgagac ggcgattgga atatggtaga agtcgacaat cagcatcttg
    13321 ataccgataa acaccagaat caccgccagg ccatatttga gcatcgagaa acgctctgct
    13381 acgcccgcca gcaggaaata catcgcacgc aggccgagga tcgcaaacag gtttgaggtc
    13441 agcacaatga acgggtcagt ggtcacggcg aagatagccg gaatgctatc cacggcaaaa
    13501 atcacgtcgc tcaattccac cagaatcagt accagcatca gcggtgtggc gtacagcaat
    13561 ccattcttac gcacaaagaa atgctcgttg tcgatggtgt cggtcatgcg caaatgaccg
    13621 cgtagccagc gcaccagcgg cttgtcgcca atgcctgatt catcttcatg ggcgagcgcc
    13681 atcttcacgc cggtaaacag caggaaggca ccgaagatat acaggatcca gtcgaactgc
    13741 gaaatcagcc agctgccagt gaagatcatg atggtacgca gaacaatcgc cccgagcacg
    13801 ccatacacca gcacgcggcg ttgtaatgcc gccggaacgg agaaatagct gaacaacatc
    13861 agccagacaa agacgttatc gaccgccagc gacttctcaa tcagataacc cgtgaggaag
    13921 gccagcgcct gtggatcggc aacggcgcga ccttcggttt gtaccagata ccaccagaaa
    13981 gcagcgttaa acagtaacga cagcgtcacc cagaccagcg accaggccgc agcctgtttc
    14041 atggtcatgg catgtgcccc acgacgtccc tgcaacaaca ggtcgatagc cagcataatg
    14101 gcgacaacaa cagcgaatcc gccccatagc aacggcgtgc cgacagtatt catagaagtt
    14161 ccttacacat aaaaaaaacg gccaacgtcg gaagacgtca gccgctgctt tttatgcata
    14221 gacctcgcct tccggcaagg tctcacttac aacaaaaaag gaatacgtct gcgtattcct
    14281 ttcgggttgc ccggtgaccg gatgtggttt ttcacacatc gtaatgacga tcgaccggca
    14341 atgaagttac tcccctttgc aggtaacaaa gtatgacaga ccattcagtc agtcaatggt
    14401 ctgttacata ccttttacac tgttttactt tggcaattat agttttacgc tatctgccgg
    14461 aaaaatcacc ccagtctggc ggcggatctc ggtcagcagt ttggctgtga tgcgactgac
    14521 cgccagccct ggatgatcca ccagatgctc atccaccagg gtagcgaaca gctctgcttc
    14581 gtagagcatg gtattaatat gctgcggctg ggtgagatcc tgcatctggc tgccacgcgg
    14641 cacgaagcac actttctggc attcagacag tttttcaatc accagcgatc ccgcttcgcc
    14701 ctgaatctcg cttgccagga cagaatcgct gacctttgag tgctgcaagg tgacgctgaa
    14761 atcaccgtaa tccatcacca ccacgccgtg ggcatctaca ccacttgcca gtaaactggc
    14821 ggttgcctgc acgcttttcg gctcaccaaa taacgctacc gccgacgcca ggcagtaaaa
    14881 gccgatatcc ataattgaac cgttagagaa tgccggatta aaggtgttgg gattctcacc
    14941 gtccaggtaa cgttgatacc gcgaggaata ctggcaatag ttgaaaaaaa ctttccgcag
    15001 tttgccgact ttcggcagcg cctggcgtaa cagatgaaag ttaggcaggc tggccgtttt
    15061 aaatgcctca aacagcacca cctgattttc acgcgcgcag gcaatggctg catccacttc
    15121 cgccagattc gacgccagtg gtttctcaca aatcacatga attttgtggc tcaggaaaag
    15181 ttgcgtctgg gaaaaatgca gggcgttcgg gctggcaata tacaccgcgt cgatagcgtc
    15241 actttccgcc attgcttcca gcgaagtaaa cagatgctcg acagaaaact cattggcgaa
    15301 gtgctgggcc tgttcaaggc tgcgggaata aacggcggtt aacttatatt taccgctttc
    15361 atgtgcggcc tccacgaact ggtgagttat ccagttcgta ccaatcacgg cgaaacgtat
    15421 cataacgcgt ggatactcca taaagggatt ctgtcgtcaa tgtagcacgc ctttttcatc
    15481 ttcgacgtgc gcggatacgc attatttctg cgccggacaa ccatgctcac taaacggacc
    15541 aataatgacc gacggcccga gatattttgg ctgacgattg aagattttcg cgtcaatcac
    15601 cgcgctgact ctggcgagtt tctctcgcag acggacaatc tttccgcgtc ctttttccag
    15661 gtctggtgcg ttaaacccga tagcatcgat gccgtattgt tttgccagcc agatggcgcg
    15721 ttggttatga aattcctgcg agataatggt gatatggttc tccccaaaca ctttattggc
    15781 acgcaccact gaatcaagcg ttgagaatcc ggcatagtca cagaagatca cttttgccgg
    15841 tacgcccttt gcgatcaaag cctgctgcat gcctgacgct tcatcgtaat ttttacgtcc
    15901 gttatcgcca ctgaccagca gccatttcac ttttccggcg tggtacaact ctgctgcggt
    15961 atcaatacgt cgggtaaagt agcgattacc cggcctggcc cccagcaata agcccacgtt
    16021 acgcgccgga acgacgttga cgtcgctcca ggtcagctgt ttgctggcgt tgaccattac
    16081 gcgatcggcg ataaaaatgg tcgctcctgc cacaagtaac agtaagcagg caatctttag
    16141 agtccggcga gaaaaacaaa tacggagaag aaggcgggca aatgcgcgca gcatggtcgt
    16201 tatccttaac gaaagggggg aggtcgattt taggcgctaa agaaagaaaa gataacgcct
    16261 gacatcattt tcagattttg tcctgcccaa gcgacaactg ggttagccac agtcgggtgt
    16321 cgaactcaag ctggtggtac tggggttcca tatgacagca caactggtaa aacgctttgt
    16381 tatgctcttt ctccttcagg tgcgccagct cgtgcaccac gatcatgcgc aaaaacggtt
    16441 ccggcgcgtt gcgaaacacg gtagcgacgc ggatctctgc ttttgctttc agcttgccgc
    16501 cctgcacacg agaaacagcg gtatgtaacc cgagcgcgtt ttttagcaca tggatcttat
    16561 tgtcatacat cactttattg atcggcgggg cattacgcag aaactgattt ttcagatcct
    16621 gagtatattg ccagagggct ttatcggtgg cgtagtcgtg tgttcccgga tagcgttttg
    16681 ccagcacatc gcccagacgc tgttcgttaa tcagtgttcg cacctgggaa agtagctgct
    16741 cgggataacc ctgaagataa gtaagattgc tcataaccgc ccacgaaaaa gagaaaaagg
    16801 gtatactcac gcaccctttt caggggcaat gtcgaaattt tatcattcag gagggccgat
    16861 gagccactta gacaacggtt tccgttcact gacactacaa cgttttccgg cgacggatga
    16921 cgttaacccg ctacaggcgt gggaagcggc ggatgaatat ttgctgcaac agttggacga
    16981 cacagaaatc cgcggcccgg tgttgatcct gaatgatgcc tttggtgcgt taagctgcgc
    17041 gctggcggaa cataagccgt acagcattgg cgactcatac atcagtgaac tggcgacgcg
    17101 cgagaattta cgcctcaacg ggattgatga gtcgagcgtg aagtttctcg acagcaccgc
    17161 cgactacccg caacagccgg gtgtggtgct gatcaaagtg ccgaaaacac tggcattgct
    17221 ggaacagcaa ctgcgtgcgc tgcgcaaagt ggtcacgcca caaacacgta ttattgccgg
    17281 tgccaaagcc cgtgacatcc acacttccac gctggaacta ttcgaaaaag tgctcggccc
    17341 gaccaccacc acgctggcat ggaagaaagc gcgcctgatt aactgcactt tcaatgaacc
    17401 accgctggcc gatgcgccgc agaccgttag ctggaagctg gaaggtactg actggactat
    17461 ccacaaccat gcgaatgtct tctcccgcac cgggctggat atcggcgcgc gcttctttat
    17521 gcaacatctg ccagagaatc tcgaagggga gattgtcgat ctcggttgcg gtaatggcgt
    17581 tattggtctg acgctgcttg ataaaaaccc gcaggcgaaa gtggtgtttg tcgatgaatc
    17641 gccgatggcg gttgcttcca gccgtttgaa cgttgaaacc aacatgccag aggcgttgga
    17701 tcgctgcgaa tttatgatca acaacgcgct ctccggcgtg gagcctttcc gctttaatgc
    17761 tgtgctctgc aacccgccgt ttcatcagca acatgcgctg accgataacg tcgcctggga
    17821 gatgttccac cacgcccgcc gctgcctgaa aatcaacggc gagctgtata tcgttgccaa
    17881 ccgtcacctg gattacttcc ataaactgaa gaaaattttc ggcaactgca ccaccatcgc
    17941 cacgaataat aaatttgtgg tgctgaaagc agtgaagctg gggcgtcgtc ggtaagattg
    18001 acagctacct gccagataag gcgtaaacgc cttatctggc atacgtttag cacattaccc
    18061 ccaggtcgga taagatgcgg caagcgtcgc atccggcaat gtctgcccag ttctggaatc
    18121 gcgctccata aaacaaaaaa tccctgtcac catcctctac attctcttgt ttagcgttgt
    18181 ttaacgttta ttcttccgtc acacagataa attcatccgt tgccaatctg tcaacggatg
    18241 ttatcatatt gccaatttgt caacggacgt gatgaagaat gcacctgata actcaaaaag
    18301 cattgaaaga tgctgcggaa aaatacccgc aacataaaac ggagttggtg gctctaggga
    18361 acacgattgc taagggatat ttcaaaaaac ctgagtcatt aaaagcagta ttcccatctc
    18421 tggataactt caaatatctg gataagcatt atgttttcaa tgttgggggc aatgaattac
    18481 gtgttgtagc aatggtcttt tttgaatcgc aaaagtgcta catacgtgaa gttatgacgc
    18541 ataaagaata cgattttttt accgctgttc atcgtactaa ggggaaaaaa tgattgcgat
    18601 tgccgacatc ttgcatgcag gagaaaagct aactgctgtg gcaccttttc tggcgggtat
    18661 tcagaacgag gaacaataca tccaggcgct ggaactggta gatcatctgc tgctcaacga
    18721 tcctgaaaac cccttactgg atctggtgtg tgccaaaata accgcgtggg aagaatcagc
    18781 gcccgaattt gcggaattta atgccatggc tcaagccatg cctggcggta tagccgtgat
    18841 tcgtaccctt atggatcaat atggtttaac cctttccgat ctgccggaaa ttggcagtaa
    18901 atctatggtg tcacgcgttt tgagcgggaa gaggaaatta acgctggaac acgctaaaaa
    18961 attggcaacg cgattcggca tttctcccgc cttgtttatt gattaacgcg
//
LOCUS       DAZFAB010000089        18516 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZFAB010000089 DAZFAB010000000
VERSION     DAZFAB010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18516
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.089"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(165..812)
                     /gene="ychE"
                     /locus_tag="ACHJWA_003506"
     CDS             complement(165..812)
                     /gene="ychE"
                     /locus_tag="ACHJWA_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFO4310036.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(843..896)
                     /gene="ychE"
                     /locus_tag="ACHJWA_003507"
     CDS             complement(843..896)
                     /gene="ychE"
                     /locus_tag="ACHJWA_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFO4310037.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            1289..3964
                     /gene="adhE"
                     /locus_tag="ACHJWA_003508"
                     /gene_synonym="adhC"
     CDS             1289..3964
                     /gene="adhE"
                     /locus_tag="ACHJWA_003508"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFO4310038.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            complement(4265..4882)
                     /gene="tdk"
                     /locus_tag="ACHJWA_003509"
     CDS             complement(4265..4882)
                     /gene="tdk"
                     /locus_tag="ACHJWA_003509"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFO4310039.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            5486..5899
                     /gene="hns"
                     /locus_tag="ACHJWA_003510"
     CDS             5486..5899
                     /gene="hns"
                     /locus_tag="ACHJWA_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFO4310040.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(6043..6951)
                     /gene="galU"
                     /locus_tag="ACHJWA_003511"
     CDS             complement(6043..6951)
                     /gene="galU"
                     /locus_tag="ACHJWA_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFO4310041.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(7153..8166)
                     /gene="rssB"
                     /locus_tag="ACHJWA_003512"
     CDS             complement(7153..8166)
                     /gene="rssB"
                     /locus_tag="ACHJWA_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFO4310042.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(8258..9163)
                     /gene="rssA"
                     /locus_tag="ACHJWA_003513"
     CDS             complement(8258..9163)
                     /gene="rssA"
                     /locus_tag="ACHJWA_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFO4310043.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            9276..9734
                     /gene="ychJ"
                     /locus_tag="ACHJWA_003514"
     CDS             9276..9734
                     /gene="ychJ"
                     /locus_tag="ACHJWA_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFO4310044.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELIAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            9784..10626
                     /gene="purU"
                     /locus_tag="ACHJWA_003515"
     CDS             9784..10626
                     /gene="purU"
                     /locus_tag="ACHJWA_003515"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFO4310045.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVSRFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            10786..10870
                     /locus_tag="ACHJWA_003516"
     tRNA            10786..10870
                     /locus_tag="ACHJWA_003516"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10820..10822,aa:Tyr,seq:gta)
     gene            10915..11045
                     /locus_tag="ACHJWA_003517"
     ncRNA           10915..11045
                     /ncRNA_class="other"
                     /locus_tag="ACHJWA_003517"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            11080..11164
                     /locus_tag="ACHJWA_003518"
     tRNA            11080..11164
                     /locus_tag="ACHJWA_003518"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11114..11116,aa:Tyr,seq:gta)
     gene            11211..11342
                     /locus_tag="ACHJWA_003519"
     ncRNA           11211..11342
                     /ncRNA_class="other"
                     /locus_tag="ACHJWA_003519"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            11389..11520
                     /locus_tag="ACHJWA_003520"
     ncRNA           11389..11520
                     /ncRNA_class="other"
                     /locus_tag="ACHJWA_003520"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(11704..12381)
                     /gene="narI"
                     /locus_tag="ACHJWA_003521"
     CDS             complement(11704..12381)
                     /gene="narI"
                     /locus_tag="ACHJWA_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO4310046.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(12381..13091)
                     /gene="narJ"
                     /locus_tag="ACHJWA_003522"
     CDS             complement(12381..13091)
                     /gene="narJ"
                     /locus_tag="ACHJWA_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO4310047.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLPKEDAHALG
                     IFLRDLTTMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLAQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVLFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(13088..14626)
                     /gene="narH"
                     /locus_tag="ACHJWA_003523"
     CDS             complement(13088..14626)
                     /gene="narH"
                     /locus_tag="ACHJWA_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO4310048.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(14623..18366)
                     /gene="narG"
                     /locus_tag="ACHJWA_003524"
     CDS             complement(14623..18366)
                     /gene="narG"
                     /locus_tag="ACHJWA_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFO4310049.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT         4684 a         4848 c         4436 g         4548 t
ORIGIN      
        1 aacatgatga tgaggagaag cagttaagga aatccttata actaactgta atttgagaag
       61 aatattttta cctattcctt ttgcttttga taactgcgcg ggaacaaagt aaaaataatg
      121 acgaagtaaa cggagcatgg cagctccgtt tcatttgaaa ggaattaatt aagcaggccg
      181 gggaaaatcc ccttaatacc agtaacgata aattcaatcc ccaatgccat cagcaatagc
      241 cccataatac gcgtaatcac gttgatgccg gtctggcgta aaacccgtac cagccacggt
      301 gccatgcgga acaatcccca acaacataaa gcgaacaatg caatagccac aaagaaacca
      361 aacagatagc taatgctgtg ataacgcgta ccccagacga tggtagaact gatcgcccct
      421 ggccccgcca tcaacggcaa cgccaatggc accacaccaa tactttcacg taccgcggtt
      481 tctgattttt cttgcttgtt ctgtttatcc tcgccaagct tgccgctgat catcgacatc
      541 gctatcgtca ccaccaggat gcccccggcg atacggaacg aatcaattga tataccaaaa
      601 agttgcagaa tcgtgtcgcc gagaaaaagc gagatccaca agataatggc cacagacagg
      661 ttggctgtaa ggttagtttt gtttcgcgct gccgctgtct gataactggt catgctgata
      721 aagacgggaa taatccctac cgggttgacc agcgcaaata acccgatgaa aaatttgaag
      781 taaacgggaa aatcaaaaag ggtctgaatc acggttagct ccgaagcaaa agccggataa
      841 tgttagccat aaataaggtt gaaaagacgc gctgacaata cgccttttga cagcattttt
      901 cacctcctaa ctacttaaaa ttgctatcat tcgttattgt tatctagttg tgcaaaacat
      961 gctaatgtag ccaccaaatc atactacaat ttattaactg ttatctataa tggcgaaaag
     1021 cgatgctgaa aggtgtcagc tttgcaaaaa tttgatttgg atcacgtaat cagtacccag
     1081 aagtgagtaa tcttgcttac gccacttgga agtgacgcat tagagataat aactctaatg
     1141 tttaaactct tttagtaaat cacagtgagt gtgtgcgcga gtaagctttt gattttcatc
     1201 ggttaagcaa atcatcaccg cactgactat actctcgtat tcgagcagat gatttactaa
     1261 aaaagtttaa cattatcagg agagcattat ggctgttact aatgtcgctg aacttaacgc
     1321 actcgtagag cgtgtaaaaa aagcccagcg tgaatatgcc agtttcactc aagagcaagt
     1381 agacaaaatc ttccgcgccg ccgctctggc tgctgcagat gctcgaatcc cactcgcgaa
     1441 aatggccgtt gccgaatccg gcatgggtat cgtcgaagat aaagtgatca aaaaccactt
     1501 tgcttctgaa tatatctaca acgcctataa agatgaaaaa acctgtggtg ttctgtctga
     1561 agacgacact tttggtacca tcactatcgc tgaaccaatc ggtattattt gcggtatcgt
     1621 tccgaccact aacccgactt caacagctat cttcaaatcg ctgatcagtc tgaagacccg
     1681 taacgccatt atcttctccc cgcacccgcg tgcaaaagat gcgaccaaca aagcggctga
     1741 tatcgttctg caggctgcta tcgctgccgg tgctccgaaa gatctgatcg gctggatcga
     1801 tcaaccttct gttgaactgt ctaacgcact gatgcaccac ccagacatca acctgatcct
     1861 cgcgactggt ggtccgggca tggttaaagc cgcatacagc tccggtaaac cagctatcgg
     1921 cgtaggcgcg ggcaacactc cggttgttat cgatgaaact gctgatatca aacgtgcagt
     1981 tgcatctgta ctgatgtcca aaaccttcga caacggcgta atctgtgctt ctgaacagtc
     2041 tgttgttgtc gttgactctg tttatgacgc agtacgtgaa cgtttcgcaa cccacggcgg
     2101 ctatctgttg cagggtaaag agctgaaagc tgttcaggac gttatcctga aaaacggtgc
     2161 gctgaacgcg gctatcgttg gtcagccagc ctataaaatt gctgaactgg caggcttctc
     2221 tgtaccagaa aacaccaaga ttctgatcgg tgaagtgacc gttgttgatg aaagcgaacc
     2281 gttcgcacat gaaaaactgt ccccgactct ggcaatgtac cgcgctaaag atttcgaaga
     2341 cgcggtagaa aaagcagaga aactggttgc tatgggtggt atcggtcata cctcttgcct
     2401 gtacactgac caggataacc aaccggctcg cgtttcttac ttcggtcaga aaatgaaaac
     2461 ggctcgtatc ctgattaaca ccccggcttc tcagggtggt atcggtgacc tgtataactt
     2521 caaactcgca ccttccctga ctctgggttg tggttcctgg ggtggtaact ccatctctga
     2581 aaacgttggt ccgaaacacc tgatcaacaa gaaaaccgtt gctaagcgag ctgaaaacat
     2641 gttgtggcac aaacttccga aatctatcta cttccgccgt ggctccctgc caatcgcgct
     2701 ggatgaagtg attactgatg gccacaaacg tgcgctcatc gtgactgacc gcttcctgtt
     2761 caacaatggt tatgctgatc agatcacttc cgtactgaaa gcagcaggcg ttgaaactga
     2821 agtcttcttc gaagtagaag cggacccgac cctgagcatc gttcgtaaag gtgcagaact
     2881 ggcaaactcc ttcaaaccag acgtgattat cgcgctgggt ggtggttccc cgatggacgc
     2941 cgcgaagatc atgtgggtta tgtacgaaca tccggaaact cacttcgaag agctggcgct
     3001 gcgctttatg gatatccgta aacgtatcta caagttcccg aaaatgggcg tgaaagcgaa
     3061 aatgattgct gtcaccacca cttctggtac aggttctgaa gtcactccgt ttgcggttgt
     3121 aactgacgac gctactggtc agaaataccc gctggcggac tatgcgctga ccccggatat
     3181 ggcgattgtc gacgccaacc tggttatgga catgccgaag tccctgtgtg ctttcggtgg
     3241 tctggacgca gtaactcacg ccatggaagc ttatgtttct gtactggcat ctgagttctc
     3301 tgatggtcag gctctgcagg cactgaaact gctgaaagaa tatctgccag cgtcctacca
     3361 cgaagggtct aaaaatccgg tagcgcgtga acgtgttcac agtgcagcga ctatcgcggg
     3421 tatcgcgttt gcgaacgcct tcctgggtgt atgtcactca atggcgcaca aactgggttc
     3481 ccagttccat attccgcacg gtctggcaaa cgccctgctg atttgtaacg ttattcgcta
     3541 caatgcgaac gacaacccga ccaagcagac tgcattcagc cagtatgacc gtccgcaggc
     3601 tcgccgtcgt tatgctgaaa ttgccgacca cctgggtctg agcgcaccgg gcgaccgtac
     3661 tgctgctaag atcgagaaac tgctggcatg gctggaaacg ctgaaggctg aactgggtat
     3721 tccgaaatct atccgtgaag ctggcgttca ggaagcagac ttcctggcga acgtggataa
     3781 actgtctgaa gatgcattcg atgaccagtg cactggcgct aacccgcgtt acccgctgat
     3841 ctccgagctg aaacagattc tgctggatac ctactacggt cgtgattatg tagaaggtga
     3901 aactgcagcg aaaaaagaag ccgctccggc taaagctgag aaaaaagcga aaaaatccgc
     3961 ttaatcagta gcgctgtctg gcaacataaa cggccccttc tggggccgtt tttttgttta
     4021 cccaaagcaa cttttccata aaccgacagc attagccttc atcatatttg cgacgatgta
     4081 taacgcctaa acacagggat attgtacttt acaggtcaca agtcaacgtc ggtgcttaag
     4141 agccctgtga ggcgtatagc ggcgttaaaa actgccgaga agggtgtata gcccggaaga
     4201 agtgcgtaaa acgaactgac aggataaaag tgccctgctc accctgtcag taaagaaatt
     4261 cttattaatc gtggcgatgc ctttcctgaa tagccgttaa tgagccgact tgtaacgcct
     4321 ctttatagtg tttacggcat acagaaacgt atcgttcatt accaccaata accacctgct
     4381 caccttcgtt ataaggtctg cctgcttgat caagacgcag caccatgctt gctttacggc
     4441 cacaaaaaca gatggttttt aattcaacca gtttgtcgga ccatgccagt aagtattggc
     4501 tgccaataaa taattcacct cgaaaatcgg tacgtaaacc ataacaaagt acgggtatat
     4561 cgagttgatc gacaacctcc gataattcat atacttgttg tctggttaaa aactggcatt
     4621 catcaaccag tacgcaatga attgcctgct gctcatgttc cgcacgaatc tcatcaaata
     4681 atgatgaatt ttggttaaat aattttgcag gcgatgacaa acctatacgc gaactgactt
     4741 tcccggcacc aaagcgatca tcaatttctg ccgtatatac gacagtgcgc atgccgcgtt
     4801 cctggtaatt gtatgaagat tgcaacaatg ctgtagactt acccgcattc attgcggaat
     4861 agtagaaata tagctgtgcc atcagccaca ggccctcaat gatatgaaat aaagttggct
     4921 ggagtttatc ataattcgtc agtttttcag ctcaacagta tgcacagaag tggtatatgc
     4981 gttctccctt acgaagcctt gcataatcct tctgagctat cattacaact gcctcgcttg
     5041 ttataagcgg gtaaatgact gctggtaact attcacaatc tttaacctgt tgcgcaagta
     5101 atagccctct gttgacctcc aggagatagt gcaatactaa gtccctgctc ttattgcgac
     5161 tgttctactt ttcatcattc gcttaatagg gaattctcgt aaacacgact aatacagaag
     5221 actgaaaggt cgtcagccta cgataatctc cccataaaat gtgacatgaa tcaggaagtt
     5281 ttaacctcac gtgctgcgaa atcatcggta caaatagggc tatatgccgc gtcttttctg
     5341 gctaatttta tgaaaagata tttattggcg gcacaaaata aagaacaatt ttgaattcct
     5401 tacattcctg gctattgcac aactgaattt aaggctctat tattacctca acaaaccacc
     5461 ccaatataag tttgagatta ctacaatgag cgaagcactt aaaattctga acaacatccg
     5521 tactcttcgt gcgcaggcaa gagaatgtac acttgaaacg ctggaagaaa tgctggaaaa
     5581 attagaagtt gttgttaacg aacgtcgcga agaagaaagc gcggctgctg ctgaagttga
     5641 agagcgcact cgtaaactgc agcaatatcg cgaaatgctg atcgctgacg gtattgaccc
     5701 gaacgagctg ctgaatagcc ttgccgccgt taaatctggc accaaagcta aacgtgctca
     5761 gcgtccggca aaatatagct acgttgacga aaacggcgaa actaaaacct ggactggcca
     5821 gggccgtact ccagctgtaa tcaaaaaagc aatggatgag caaggtaaat ccctcgacga
     5881 tttcctgatc aagcaataat cttttgtaga ttgcacttgc ttaaaatccc gccatcggcg
     5941 ggatttttta ttgtccggtt taagacaatt taataagaat aagctataaa aaaacggcgt
     6001 cgattgctca acgccgtttc gtggataaca ccgatacgga tgttacttct taatgcccat
     6061 ctcttcttca agccaggctt taaattccgt gccaagggtg ttatgacgaa taccgtattc
     6121 aacgaaggcc tgcatgtaac ctaatttatt accgcagtca tggctcttcc ctttcatatg
     6181 ataggcttca accgtttctt tttcgattag catatcaatt gcgtcggtga gctgaatttc
     6241 atcaccagct cccggagggg tttttgccag caacggccaa atatccgcac taagtacgta
     6301 acgacccaca atagcgagat tagacggcgc aacatccgct tttggttttt caaccacgcc
     6361 aaccatcggt acgctttcac ccggcgctaa ttcaacgcct ttgcaatcca caacgccata
     6421 tgcggtcaca tcagcaaccg gttcaaccat gatctggcta tgacccgttt catcgaagcg
     6481 gcggatcatc tctgccaggt tatcctgtga caaatcggat tcatattcat ccagaataac
     6541 gtcaggcaaa ataacagcca caggttcatc acccactacc gggtgagcac acaataccgc
     6601 gtgtcccagg cctttcgcca gcccctgacg aacttgcata atagttacgt gcggcggaca
     6661 aatagactgc acttcatcaa gcagttgacg ttttacacgt ttttccagca ttgcttccag
     6721 ttcaaaactg gtatcaaagt ggttttcaat agagttttta gatgagtgtg taaccagcac
     6781 aatttcagta atgccagccg caatacattc attcacgacg tattgaatta atggcttatc
     6841 gacaagtggc agcatctctt tcgggatggc tttcgtcgcc ggcaacatcc tggttcctaa
     6901 tcccgcaacg gggataacgg cttttttgac tttcgtatta atggcagcca tttaaattct
     6961 cctggactgt tcgtgttttg aacgtgttca tatttctgta tcgcatccca gtatatcagc
     7021 accccggagg ggctaagttc tgaaaaggat gcgagttagc ataattaaga caattactta
     7081 tacgaatggc gacgatagta ccaccagccg acattagctg gtaatgcaaa tttagcccgc
     7141 gttatcgttt gctcattctg cagacaacat caagcgcagt cgaccaccgg ttccccatat
     7201 ttggcattgc caggcatcgc atcgctggct caattgattc aaataagcgt tacctaaagt
     7261 gcctaacgga acaccattac tgatttgcac ctggtgttcg ccggtattta acgtcgcatt
     7321 cagacccgca gaaaccagaa tgagattttt cagttcgcga tgataatagc caactaatag
     7381 cggaaactgc cccggcagat tggcctgacg gagtaaatgg tttacctgct tcaataacgc
     7441 gcccaactct ggcaaccgtt gattttggtg tgcaagctgt tcctgtaata atccgttaaa
     7501 caatgcgcgt aataacaagg cagcaagtac gccattatgt ccagctcggg ttacatcaag
     7561 gcaataaaat gccagatcgt tttccgaaag tgcggcaata tcaagcacca ggccgggttt
     7621 gtccgcggca accaattgac ggtaattaac ccggcaatgg gaaatcacct gctgaaccgg
     7681 cggttgtagt tcctgtaata atttcgccgc tgcggcaggg ttatcaacca ttgcatccca
     7741 gtcgcgaaaa agcctttcct cttcctcaac gcgcgaatta aacatgctgg gatagagaca
     7801 ggcaaaaacc atctcgcgca agcgattcag atctttaact ggtttcagca aaacatcttc
     7861 aacgcccaga cgtaacgctt tggcaatatc tgccatattt tcagtggcag atatcaccag
     7921 aactggggtc tggtcgcctc tgttacgtat atgctccagc agtttaagcc cgttcattcg
     7981 tggcatcgcg atatcacata tcatcaggtc tggagtgaaa cctcccagca actcaagggc
     8041 atccacccca tcagccgcca gtaccgttgt cgctcccaat gaggaaaacc acgaatccag
     8101 aagcgagcga aatacctgct catcttcaac aatgagaatc tgttttccga ccaatggctg
     8161 cgtcatgttc tctcccctga ctggctttac tcaatagtgg catgctattg ccacctgcgc
     8221 ctgtcagaat taacttaagt gtagataaaa attctggtca aatgttggtg cgtaccaacg
     8281 gcaaaagttc gtccattttc ttttccactg ccagctgccc ggccgcaatg gcagcgtgcg
     8341 cgcgatggaa atcaagcgta gatatttgcg ggcaaacagg ttgaatcaga atatcgggcg
     8401 gatcacctgc catgcggttc cttttaaggc ggttctccag cacctggata gaagtggtca
     8461 taatctctgt tgccgttggt gctgtcaccg cccgacgtgt cgttatactg cctaaccttt
     8521 ctttcagacg cgcatgccac ggcagagaat caccattctc gctattttct tcactgacat
     8581 taaaggagag caaatcttgt tgcatcaaat gagcatcgtg ctgcaggtcg accgctatca
     8641 caatatcagc acccaatgca cgcgtgagcg aaataggaat tgggttaacg actgccccat
     8701 caaccagcca gtagccgtta tgcgcaacgg gtgccattag tcctggaata ctgcatgatg
     8761 cgcgaatagc aagatggaga tcgccttcag taaaccataa ttcacgccca gtacttaaat
     8821 tggtggcaac agccgcgaag cgacgggaac aattttcgat ctctgtttcc ggcattattt
     8881 cgcggtattg attgaagaca cgctcgccgc gcagtaaccc accgcgctgc caggagagat
     8941 ccatcagtcg taaaacatcc caataactga aagaggtcac ccaatcttcc agcgcagata
     9001 atcgatcgca tgcataggcc gcgcccacca gcgaaccaat tgaacatcct gcaacgatat
     9061 caatttcaat acccactttt tttagcgcat taataacgcc aatatgcgac caacctctcg
     9121 ccgcgccaga tcccagcgcc agccctattt ttatctttct cattattccc gcaagattcc
     9181 cctggaatgc aatcgtagcc acatcgctac tgctcggtta acatagtgct actcaggcgg
     9241 ttaatacgcc gttattgttt ccagggagat tatttgtgtc tcagctttgt ccctgtggta
     9301 gtgctgtcga gtatagccta tgttgccacc cttatgtgtc tggtgaaaag gttgcacctg
     9361 atccagaaca tctcatgcgt tcgcgttact gcgcttttgt gatgcaagac gcagattatt
     9421 taataaagac ctggcatcca tcttgtggag cagcggcgtt acgtgccgaa ttgattgccg
     9481 gatttgcgca tactgaatgg ttgggattga ccgtatttga acattgttgg caggacgccg
     9541 acaatatcgg atttgtcagt tttgttgctc gatttaccga aggtggcaaa acaggtgcaa
     9601 taattgaacg ttcgcgattt ttgaaagaaa acggtcaatg gtactatatt gacggtacac
     9661 gtccgcagtt tggccgcaac gatccctgcc cttgtggttc aggtaaaaaa tttaaaaagt
     9721 gctgcggcca ataatggttg acggtacggt ttagcaaaca ctctcaacaa ggtttttcca
     9781 gcaatgcatt cactccaacg taaagttctg cgtactattt gtccggacca aaaaggtctg
     9841 atcgcacgta ttaccaatat ttgctacaag cacgagttaa atatcgtaca gaacaatgaa
     9901 tttgttgatc accgtaccgg gcgctttttt atgcgcacgg aactggaagg gatttttaat
     9961 gattccaccc tgctggcgga tctcgatagc gcattgccag aaggctccgt gcgtgagctg
    10021 aatcctgccg gtcgtcgccg gatagtgatt ctggtcacta aagaagcgca ttgccttggc
    10081 gatttgttga tgaaagccaa ttatggcggc ctggatgtcg aaatcgcggc agtgattggt
    10141 aaccacgata ctttacgttc tctggttgag cgttttgata taccgtttga gctggtaagc
    10201 catgaagggt taagccgcaa cgagcacgat caaaagatgg cggatgccat tgatgcttat
    10261 caacctgact acgtggtgct ggcgaagtat atgcgggtat taacaccgga atttgtgtca
    10321 cgcttcccga ataagatcat caatattcac cattccttcc tgccagcgtt tatcggcgca
    10381 cgtccttatc accaggccta tgaacgtggc gtgaagatta ttggcgcaac cgctcactat
    10441 gtgaatgaca atctggacga aggcccaatc atcatgcagg acgttattca tgtcgatcat
    10501 acctacacag ctgaagatat gatgcgcgca ggtcgtgacg tcgagaaaaa cgtcttaagt
    10561 cgcgcgctct acaaagtact ggcgcagcgc gtctttgttt acggtaatcg gacgattatt
    10621 ctttaatcgc cagcaaaaat aactggttac ctttaatccg ttacggatga aaattacgca
    10681 accagttcat ttttctcaac gtaacacttt acagcggcgc gtcatttgat atgatgcgcc
    10741 ccgcttcccg ataagggagc aggccagtaa aaagcattac cccgtggtgg ggttcccgag
    10801 cggccaaagg gagcagactg taaatctgcc gtcatcgact tcgaaggttc gaatccttcc
    10861 cccaccacca tgattcacca cagcgatgtg gtaacttcgg tgagattgtg aaagtttgca
    10921 ctcatcgggg aagggtgaga accttcgact aaggttcgac tcgagcgaaa gcgagagaac
    10981 attttcacag gcaacggccc gaagggtgag gagcgaagcg acgaataatc ctttccccac
    11041 cgccatctca agcattacct caaaatttga attacccctg gtggggttcc cgagcggcca
    11101 aagggagcag actgtaaatc tgccgtcatc gacttcgaag gttcgaatcc ttcccccacc
    11161 accatcactt tcaaaagtcc ctgaactctc aagcgaatcc gtaaataaat attctgccca
    11221 agcggagaag gatgagaagc ttcgactaag gttcgactcg agcgccagcg agagagcgtt
    11281 gccgcaggca acgacctgaa gggcgaagcg cgcagcgctg agtaatcctt cccccaccac
    11341 catcactttc aaaagtccct gaactctcaa acgaatccgt aattaaatat tctgcccaag
    11401 cggggaagga tgagaagctt cgaccaaggt tcgacttgag cgccagcgag agagcgttgc
    11461 cgcaggcaac gacccgaagg gcgaagcgcg cagcgctgag taatccttcc cccaccacca
    11521 tcgcttcctg aaataatcaa aacctgttac tccgagctaa ttttgcccaa tggcaccacg
    11581 tttaccggac ctacaatatc ctcccttgta ggtcggataa gcgaagcgca tccgacaact
    11641 caccccgcac cgtgcccata aaaaaacccc gccgaagcag ggtctatgtg aactaaaatt
    11701 cgcttagtga cgagcgcgca ccagctggta cttacgtgtc agatactcca ccggtacgct
    11761 ccagatgtgt accagacgcg agaacgggaa cagcaggaat aacgtcatcc ccagcaccag
    11821 gtgtagacgg aagataaacg ccacaccatc gaggtgttga gaagcgccac cgtggaaggt
    11881 cactaccgac tgcgcccagc caaccagttt catcatctcg ctaccgtcca tatgttgggc
    11941 ggagaacgga atggtcaaca ggcccagcgc gcactggata acgagcagcg acaggatcag
    12001 tatatccgct ccggtagtcg ttgcacgcac gcgtgggctg aacagacgac gtttaagcaa
    12061 aagcacgccg ccaatcagac acagcacgcc gctggcacca ccagcaaaca tcgccatttt
    12121 ctgtttcact tcaatcggca gccaggcttc atacatccag tgcggcgtca acataccgaa
    12181 gaagtgaccg acaaaaatcc ccagaatacc gatatggaac aggttcgacg ccaggttcat
    12241 ccctttgcga tccagcatct ggctggacgc cgcgcgccag gtgtactgcc cgtagtcata
    12301 acgcagccag ctaccaatca ggaagaccgc cccggctatg tacgggtaga tatcaaagaa
    12361 gaacatattc aggaattgca ttagtgctgt cctccggtgg tgatattcag atattgcggc
    12421 gcgacggcac cggcaaagcg acgctgatga gcagtgattg ctgaatcacc gcagcctttg
    12481 tcagcaaaga atttaacctg ctcttcttcc caaacagcat ccagcgcctg cggcgtatca
    12541 tcgcgcgctt cgtcggcaat tttttccgcc actttgtcgc tgtcgatagc ggtattggcc
    12601 aatttcagca gcagatcaaa cagcacggca taacggcttt cacgctgttg cagacgcgca
    12661 ctcagcaatg ccagaatcgg cgcgatatct ttcaaacctt ccacggcttc gctttgcggc
    12721 agctgcgcca ggtactccag atacagcggc agatgatccg gcaattcgcg gctgtttaac
    12781 tgcaagccgt gctgctcgta ctgcgccagc aggtccacca tcgcctggcc gcggtcgcgg
    12841 gattcgccgt gcacatgttc aaacagcaac agtgacgtgg cgcggccacg gtcgaacagt
    12901 tcgctgtact gcgcctgggc atcgagcgga tccatcgtcg ttaaatcgcg caggaaaatg
    12961 cccagcgcat gggcatcctc ttttggcaga tttttcgacg cggcaatcgc ctcaaacatc
    13021 tcttgttgat gctgccataa ggcagcatcc ggatattcaa ggagacgcga tacaatcacg
    13081 agttcgatca tggatgcggc tccgttttgc tggtcacatc gatggcatcg atacgacggc
    13141 tgttgaacag attgaatttg gtatctgaac cgtggcagcc atcaccaaag gtaaagccgc
    13201 agccattttt ctccgggaag gcttcccgtg ccagttcacg atgactactc ggcaccacaa
    13261 agcgatcttc gtagttagca atcgccagat aacggtacat ctcctgtgcc tgggcttcgg
    13321 tcagaccgac ctcttccagc gcacgggtat caactttacc gtcaacggtt tcagcacgtt
    13381 tgtaatgacg catcgccagc atacgtttca gcgcgcgcag taccggtttg gtatcaccgg
    13441 cggtcagcag attcgccaga tactgtaccg gaatacgcag gctttcgacg tctggcagaa
    13501 tgccgttgct acccagctca cccgcgtctg ctgcagactg aatcggagag agaggcggca
    13561 cgtaccagac catcggcagt gtgcgatatt ctggatgcag cggcagcgcc agtttccatt
    13621 ccattgccat tttataaacc ggcgactgct gtgcagcttc aataacgctc agcggaatac
    13681 cgtctttaat cgcctgctcg atgactttcg gatcgttcgg atcgaggaac acctccagct
    13741 gacgctggta gagatctttc tcgttctcgg tgctggctgc acgttcaatc gcgtcggcat
    13801 cgtacagcag cacgccaaga taacggatac gaccgacaca ggtttctgag cacacggtcg
    13861 gctgacccgc ttcaatacgc ggatagcaga agatgcactt ctcagactta ccgctcttcc
    13921 agttgaagta gatttttttg tacgggcatc cggtgatgca catacgccag ccacggcatt
    13981 tatcctggtc gatcaggacg atgccatctt cttcacgctt gtaaatcgca ccgctcgggc
    14041 aggtcgccac acatgccggg ttcaggcagt gttcgcacag gcgcggcaaa tacatcatga
    14101 aggtgttttc gaactggcta tacatcgcct tctggatgtt gtcgaagttc ttgtctttcg
    14161 ccagtttgtc aaactcaccg cccaggtcat cttcccagtt cggccctttt tcgattttcg
    14221 ccatccgttc cccggtaatc agcgaacgcg gacgggcaat cggctgcgat ttgctgcctt
    14281 ccggcgcggt atgcaggttc tgatagtcaa aatcgaacgg ctcgtaataa tcgtcgatcc
    14341 ccggcagatg cgggttagcg aagattttac ccagcagcat ggcacggtta cccatgcgcg
    14401 gttgcagttt gccgttgatt ttacggatcc agccgccttt gtatttttcc tggttttccc
    14461 agtcagtcgg gaagccctgg cccggcttgg tttccacgtt gttgaaccac gcgtattcca
    14521 cgccttcacg gctggtccag acgtttttac aggtaactga acaggtgtgg cagccgatgc
    14581 acttatcgag attcagcacc atgccgactt gtgaacgaat tttcatttta cgctctcctg
    14641 tacctgatca ttgccttcgc catctaacca gtcaatgttc ttcatcttac gcactacaac
    14701 aaactcatcg cggttagaac ctacggtgcc gtagtagtta aagccgtatg ccagatgggc
    14761 atagccgccg atcatatgcg tcggtttcgg cgtgatacgg gtgaccgagt tatggatacc
    14821 accacgctgt tgggtaattt ccgaaccagg caggttaacg atacgttcct gcgcgtggta
    14881 catcatggtc atccctgccg gaacacgctg gctgacaacc gcacgggcag tcagagcacc
    14941 gttgctgttg aatacttcaa tccagtcgtt atcggcgata cccagatctt tggcatcggc
    15001 ttcactcaac cagaccaccg gaccaccgcg acctaaagtc agcatcagca ggttgtcgct
    15061 gtaggtggag tggatacccc acttctggtg cggcgtcagg aagttgagcg ctttttccgg
    15121 gttgccgttg gatttctggc ctatcacttc tttcaccgaa cgggtgtcga tcggcggacg
    15181 ataaaccagc aggctttcac cgaaatcacg catccactgg tgatcctgat acagttgctg
    15241 acgaccagag agcgtacgcc atgggatcag ctcgtgaacg ttggtgtaac cggcgttgta
    15301 agaaacgtgt tcatcttcca gaccagacca ggtcgggctg gagataattt tgcgcggctg
    15361 tgcctgaata tcgcggaagc ggatcttctc gtcttcttta ttcagcgcca gatgcgtatg
    15421 gtcacgaccg gtaaattcgc tcagggcagc ccaggctttc acggctacct gaccgttggt
    15481 ttccggtgcc agtgtcagga tcatctctgc cgcatcaatt gcggtgttca gcatcggctg
    15541 gcctttcgcc ggaccttccg ctttggtgta gttgagctta cgcagcagat ccatctcgct
    15601 ctgggtgttc caggcaatcc ctttaccgcc gttaccgatt ttctccatca gcgggccgat
    15661 agaggtaaag cgttcgtaag ttgccggata atcgcgctct acgaccataa tgtgcggtgc
    15721 agttttgcct gggatcaggt cgcattcgcc ttttttccag tctttcacat ccagcggctg
    15781 cgccagttca gcggcagagt cgtgctggat aggcagcgtg acgatgtcgg tttctttacc
    15841 cagatggccg acgcacactt cggagaattt cttcgcgatg gctttgtaga tttcccagtc
    15901 gcttttcgct tcccaggccg gatcgaccgc cgcagacagc gggtgaataa acggatgcat
    15961 atccgaagta ttcatgtcgt ctttttcgta ccaggtcgcc gtcggcaaaa tgatgtcgga
    16021 atagagacag gtgctcgaca gacggaagtc cagcgtaacc accagatcca gcttgccttc
    16081 cagaccattg tcctgccagt ccacttcttc cggcttcacg ccgccctgct gccccagatc
    16141 tttaccctgg ataccgtgct ccgtccccag caggtacttg agcataaact catgaccttt
    16201 accggaagaa ccgagcaggt tagaacgcca gataaacagg ttacgcgggt ggtttttgcc
    16261 gttttccggc tgttccgcag cgaaacggat agaaccctct ttcagggatt tcaccgtata
    16321 gtccaccgga ttcatcccgg ctttttctgc ttcgccagcg atagtcagcg ggttagtgcc
    16381 taactgcggc gcagacggca gccagcccat gcgttctgca cggacgttaa agtcgatcag
    16441 atgtccggta tagcgggatt tatccgccat cggcgacagc agttcttccg cagtcacggt
    16501 ttcataacgc cactggctgg agtggttata gaaataagag gtgctgttca tgtgacgcgc
    16561 cggacgctgc cagtcgaggg caaacgccag cggctgccag ccggtttgcg gacgcagttt
    16621 ttcctgacct acatagtgcg cccagccgcc cccgctctga ccgacacagc cgcagaaaat
    16681 cagcatgttg atcagaccac gatagttcat atcgaggtga taccagtggt tcagccccgc
    16741 accgacgata atcatcgaac gaccgtgcgt tttatcagcg ttatcggcaa attcacgggc
    16801 gatgcgaata atctggctgc gagaaacgcc ggtaatctgc tcggcccagg ccggggtata
    16861 agctttcaca tcgtcatagc tggttgcaca gttaacgtcg ttcaggccac gttccagacc
    16921 gtagtttgcc agcgtcagat cataaacggt ggtcaccagg gcggtgctgc catcagccag
    16981 ttgcaggcgt ttcaccggca gtttgtgcag cagcacgttt tccagttcca ctttgttgaa
    17041 gtgttccgtg ccgtcgccac caaagtacgg gaagcctacc tcagcgatct catcctggct
    17101 acccagcagg ctcagttgca gctcggtttc ttcgccagtt ttgccgtcgc gctgctcaag
    17161 attccatttg cccttctcgc cccagcggaa gccaatagaa ccgttcggcg caaccatttc
    17221 gccattggta ttaaaggcga cagttttcca ttccggattg ttttcctggc ccagcgcatc
    17281 aaccagatca gcagcgcgca gcatacgacc tgcagcgtag taaccgtcgc gttcttccag
    17341 catcaccagc atcggcatgt cggtgtagcg acgcacatag tcggtgaaat actggcttgg
    17401 gttgtcgaga tggaattcac gcagcattac gtggcccatc gccagcgcca ttgccgcatc
    17461 ggtgccctgt ttcggtgcca gccacagatc gcacagtttg gcgatttcag cgtagtctgg
    17521 tgtgacggca acagttttgg tgcctttgta acgcacttca gtaaagaagt gagcatctgg
    17581 ggtacgcgtc tgcggcacgt ttgaacccca ggcgatgatg tagctggagt tgtaccagtc
    17641 agcagattcc ggtacgtcag tttgctcgcc ccaggtttgc ggagacgcag gaggcaggtc
    17701 gcagtaccag tcgtagaagc ttaagcaagt accgccaatc agcgagagat agcgtgcacc
    17761 cgatgcgtaa gaaaccatcg acattgccgg aattggcgag aaaccagcaa cacggtccgg
    17821 gccgtagttt ttgatggtgt aaacgttaga tgcggcgatc agttcgttca cctcctgcca
    17881 ggaagaacga acaaaaccac cgcgtccacg cgcctgctta aagcttttcg ctttatcggc
    17941 gtcttcaatg atagaagccc atgcctcaac cggatcgcta tgcagcgcct tcgcttcacg
    18001 ccacattttc atcaggcgtt tgcgcatcat cgggtatttc aggcggttgg cactgtaaag
    18061 ataccaggag tagctggcac cgcgcgggca gccgcgaggt tcatggtttg gcagatccgg
    18121 acgggtacgc ggatagtcag tctgctgggt ttcccaggtg accagaccgt ttttgacgta
    18181 gattttccag ctgcaggagc cggtgcagtt taccccgtgg gtagagcgga cgattttgtc
    18241 atgctgccaa cgctggcgat atccatcctc ccagtcacgg ttggtattga gaagctggcc
    18301 atgcccatcg gcaaaggttt cacccttctg tttgaagtag cgaaaccggt ccaggaattt
    18361 actcatcggt tttctcctgt gggagcctgt cggccctctg ataaatcgac attgctaatg
    18421 tttaagggca aggtaacgct ctgaaacagc gtgggaattg ataacgatca agagtgatgg
    18481 ggatgaaaaa taaagtaaat tcctaagata cccctt
//
LOCUS       DAZFAB010000090        18516 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZFAB010000090 DAZFAB010000000
VERSION     DAZFAB010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18516
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.090"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..620)
                     /locus_tag="ACHJWA_003525"
     CDS             complement(<1..620)
                     /locus_tag="ACHJWA_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFO4310050.1"
                     /translation="MQKRVLPIDVYSDETDEPRLIVRKKEHDLYKYYILQDSKDYKPE
                     KPSDTELVFISNAETIRDYLLRLSVDELKLLAKYILQNVYIVFVQTDDFASSFRLFNV
                     LNSRGLPLSNADLLKNALFESASTHNKKSEQIESAWSQIEDMVGVRRLDKFLTLHKLS
                     EKKDRDRVLQKGFEAFIENLQQQFDGDAIAMSLMLVNSAKNYTKIL"
     gene            complement(629..913)
                     /locus_tag="ACHJWA_003526"
     CDS             complement(629..913)
                     /locus_tag="ACHJWA_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFO4310051.1"
                     /translation="MEAKECKVQDILTENKKFIIPSYQRPYSWTVDNAEQLIDDIYKS
                     SQSEENGYFIGSMICINKGQNQYEVVDGQQRLTTLSIIVSELKKSSRFRG"
     gene            1191..3623
                     /gene="hsdR"
                     /locus_tag="ACHJWA_003527"
     CDS             1191..3623
                     /gene="hsdR"
                     /locus_tag="ACHJWA_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EcoAI/FtnUII family type I restriction enzme
                     subunit R"
                     /protein_id="HFO4310052.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPEQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVKDTDDVGPLPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVRKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            3690..5159
                     /locus_tag="ACHJWA_003528"
     CDS             3690..5159
                     /locus_tag="ACHJWA_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFO4310053.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSRDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPAELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            5159..6913
                     /locus_tag="ACHJWA_003529"
     CDS             5159..6913
                     /locus_tag="ACHJWA_003529"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO4310054.1"
                     /translation="MSVEKLIVDHMKTWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASELLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVRISEIGHDWGQKTPDKDFTYIDVGSINKEYGIIEELSILSAKDAPSRARKIV
                     QQGTIIYSTVRPYLLNIAIIENEILPEPIASTAFAIIHPYTAMDANFIYYYLRSPVFV
                     CYVENCQTGVAYPAINDKQFFSGITPVPPSLEQVRIANKIKELMSLCDQLEQQSLTSL
                     DAHQQLVETLLGTLTDSQTAEELAENWARISEYFDTLFTTEASVDALKQTILQLAVMG
                     KLVPQDPNDEPASELLKRIAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCL
                     FEDIIDIQSGITKGRNLSNRTLVKVPYLRVANVQRGYLDLTEIKQIEIPIEEKEKYQV
                     VKGDLLITEGGDWDTVGRTTVWCHDWYIANQNHVFKGRNIGQDVDPYWLETYMNSPFS
                     RQYFANASKQTTNLASINKTQLRGCPVAIPPSSEAKKIMSKLHIFYKLCEELKNHIQS
                     AQQTQLHLADALTDAAVN"
     gene            7134..7475
                     /gene="symE"
                     /locus_tag="ACHJWA_003530"
     CDS             7134..7475
                     /gene="symE"
                     /locus_tag="ACHJWA_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HFO4310055.1"
                     /translation="MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTVVDVKVMEGCIVLTAQPPAAAESELMQSLRQVCKLSARKQRQVQEF
                     IGVIAGKQKVA"
     gene            complement(7522..8685)
                     /locus_tag="ACHJWA_003531"
     CDS             complement(7522..8685)
                     /locus_tag="ACHJWA_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1524 domain-containing protein"
                     /protein_id="HFO4310056.1"
                     /translation="MALGKESDKSLATAFQDLRELKVDVAYPFLLALYHDYKNDDLSH
                     EDFLSIIRLIESYVFRRAVCAIPTNSLNKTFATFYKVINKEKYLESIQVHFLNLPSYR
                     RFPNDDEFKRELKVRDLYNFRSRSYWLRRLENDKRRERVEEFTIEHIMPQNENLSAKW
                     REELGSDWQRIHKELLHTLGNLTLTRYNSRYSDRPFAEKRDIEDGFKHSPLYLNIGLG
                     QCEKWDEAAIHARADRLAELAVQVWQAPSLPEEVLAVYRGQPENKTSYSLSDYPFLAD
                     GSHSRVLFDHLRDEIMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLRLSLNMQFH
                     ELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMESALV"
     gene            complement(8733..9614)
                     /locus_tag="ACHJWA_003532"
     CDS             complement(8733..9614)
                     /locus_tag="ACHJWA_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFO4310057.1"
                     /translation="MKATEARLLDFLKRSQQFVIPIYQRTYSWTEQQCRQLWDDIIRA
                     GKRDDISAHFIGSVVYIEQGLYQVSGISPLLVIDGQQRLTTAMLLIEALSRHLGEDEV
                     FDGFSAMKLRNYYLLNPYESGEKGFKLLLTETDKDSLLALIKQRPMPENYSHRIMENF
                     TFFDEQIAKLGDDLIPLCRGLAKLLIVDVALNRGQDNPQLIFESMNSTGKALSQADLV
                     RNFILMGLEPEHQTRLYEDHWRPMEVACGQQGYSEYFDSFMRHYLTVKTGRSLGQMKS
                     MRHLNSMPAARVLLKKA"
     gene            complement(9711..9875)
                     /locus_tag="ACHJWA_003533"
     CDS             complement(9711..9875)
                     /locus_tag="ACHJWA_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HFO4310058.1"
                     /translation="MEFHENKAKTPFIRLVQLWQAVRRWRRQMQARRVLQQMSDERLR
                     DIGLRREDVE"
     gene            10052..11464
                     /locus_tag="ACHJWA_003534"
     CDS             10052..11464
                     /locus_tag="ACHJWA_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFO4310059.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMKLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDLVLDMLVAHS
                     DSSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQVARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLM
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQIYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVTRQLCRMKIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYRAME"
     gene            complement(11707..12664)
                     /locus_tag="ACHJWA_003535"
                     /pseudo
     CDS             complement(11707..12664)
                     /locus_tag="ACHJWA_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887616.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
     gene            13131..14363
                     /gene="mdtM"
                     /locus_tag="ACHJWA_003536"
     CDS             13131..14363
                     /gene="mdtM"
                     /locus_tag="ACHJWA_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HFO4310060.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRKPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEEQ"
     gene            14404..15675
                     /locus_tag="ACHJWA_003537"
     CDS             14404..15675
                     /locus_tag="ACHJWA_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HFO4310061.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQKKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMTARADAVKSYLKEDEAFLSELWGDLREWLKADINSEDSRVKERIARAGQWFGETLI
                     ADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKDLQF
                     IRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            15791..16942
                     /locus_tag="ACHJWA_003538"
     CDS             15791..16942
                     /locus_tag="ACHJWA_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HFO4310062.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKVRGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKAVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQFNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARIRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLKMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            16952..17719
                     /gene="yjiL"
                     /locus_tag="ACHJWA_003539"
     CDS             16952..17719
                     /gene="yjiL"
                     /locus_tag="ACHJWA_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HFO4310063.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDCITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLRMPVNTHPDAQFAGTIGAAVIGQRVRTRR"
     gene            17716..17982
                     /locus_tag="ACHJWA_003540"
     CDS             17716..17982
                     /locus_tag="ACHJWA_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HFO4310064.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGQTEAIYCQQGSDWQCVVHYDAEASTRQ"
     gene            18294..>18516
                     /locus_tag="ACHJWA_003541"
     CDS             18294..>18516
                     /locus_tag="ACHJWA_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HFO4310065.1"
                     /translation="MGSLFNIYKDIFPTLGMYSGLKACHEKNNLPFDINTEIETIQKQ
                     INYDINHLNDGLIKRVLNLFIHLISNPDNL"
BASE COUNT         4897 a         4490 c         4363 g         4766 t
ORIGIN      
        1 tctaaaatct tagtataatt ttttgcagaa ttaaccaaca tcagtgacat agcaatagca
       61 tctccatcaa attgctgctg gagattttca ataaaagctt caaaaccttt ttgtaacacc
      121 ctgtcacgat cttttttctc ggaaagctta tgaagagtaa gaaatttatc aagccgtctc
      181 actccgacca tatcttcaat ttgcgaccat gctgattcta tttgctctga ttttttatta
      241 tgtgtagaag ctgattcaaa cagtgcattt tttaaaagat ctgcattact taaaggtaaa
      301 cctcgactat ttaatacatt aaagagtcta aatgatgatg cgaaatcatc tgtttgcaca
      361 aatacgatat atacattttg caaaatatat ttagcaagca acttcaactc atcaacagat
      421 aaccgcaaca aataatctct aattgtttca gcgttgctaa tgaatactaa ctcagtatcc
      481 gatggctttt caggtttata atctttagaa tcctgcagta tataatattt ataaagatca
      541 tgctcttttt ttctaacaat tagtcttggt tcatctgttt catcagaata aacatcaata
      601 ggcagaactc ttttttgcaa atcatctttt atcccctgaa tcgggatgat ttttttagct
      661 cggagacaat aatgcttaac gttgtaagac gttgttgtcc atctacaact tcatactgat
      721 tttgcccttt attgatacaa atcatactcc caataaaata tccattctct tctgattgag
      781 agcttttata aatgtcatca atgagttgct ctgcattatc taccgtccaa ctgtaaggac
      841 gctgataaga aggaataata aactttttgt tttcagtaag gatatcctgc accttacatt
      901 ctttagcttc catttcatat acccaatcaa acaatcaaca ataacattat ctttcttgct
      961 cgaaagaaac gcaactataa gatttttata tattttgtta gagttgatat ggttcctgta
     1021 acttctgttt atctgtctct gatatgccca gattaattca agcaagatat aacagagata
     1081 tcaatgtgat ctctgctacg tactttacgt tgaaacaccg ccctaaatca tagccaggct
     1141 attgatttta tgtacaatca gaatcgtcct gataaaagcg agaatacccc atggcggaac
     1201 tgaacctaag taacctgacg gaagcagaca tcattaccaa atgcgttatg ccagccattc
     1261 tcaatgcagg ctgggacaac acaacgcaaa tcagacagga ggtcaaactc cgggacggta
     1321 aagtcatcgt acgtggtaaa gttgcggcac gcagaacggt aaaatctgct gacatcgtgc
     1381 tgtatcacaa acctggcatt cccttagccg tgattgaagc aaaagccaac aaacatgaaa
     1441 ttggcaaagg gatgcaacag ggcattgaat atgcgcgcct gctggacgtt ccttttgttt
     1501 ttgccaccaa cggtgatggc tttatcttcc gcgatgccac cgcagccgaa ggtgaatgcc
     1561 ttgaaaagca aataacgctg gatgacttcc cctcccccgc tgaactctgg caaaaattct
     1621 gcctctggaa aggatacaca caagctcagc ttccggtgat tactcaggac tattacgacg
     1681 atggcagcgg taaatcgcca cgttattacc agcttcaggc aatcaacaaa accattgaag
     1741 ccgtctccaa cgggcaaaac cgcgttctgc tggtcatggc gaccggaaca gggaaaacct
     1801 ataccgcatt ccagatcatc tggcgcctgt ggaaatcaaa aaataaaaaa cgcattttgt
     1861 tccttgccga tcgcaatatt ctggtcgacc aaaccaaaaa taatgatttc cagccatttg
     1921 gtacggcaat gaccaaagtc agcggacgca ccattgatcc cgcttatgaa attcacctcg
     1981 cgctctatca ggctataact ggcccggagg aagaccaaaa agcgtttaaa caagtcgcac
     2041 cagatttctt cgatctgatc gtgatcgacg aatgccatcg cggcagcgca tccgaagaca
     2101 gcgcctggcg agaaatcctt gattatttta gttccgccac ccaaattggc ttaaccgcca
     2161 cgccaaaaga gacgcatgaa gtctccagca cggattactt cggcgatccg gtttacgtct
     2221 actcactaaa agaagggatc gaagacggct tcctcgcccc ttataaagtt gtccgtgttg
     2281 atattgatgt tgatctgcaa ggctggcgcc caaccaaagg gcaaactgac ttaaacggcg
     2341 aagtgatcga cgatcgtatc tataaccaga aagatttcga tcgcacgatg gtaatcgacg
     2401 aacgcacaga actggttgcc agaaccatta ccgactacct caagcgtacc aatccgatgg
     2461 ataaaaccat cgtcttctgt aacgacatcg atcatgcaga acgtatgcgc cgcgccctgg
     2521 ttaatctcaa cccggagcag gtgaaaaaga acgacaaata cgtcatgaaa atcaccggcg
     2581 atgatgaaat tggcaaagct cagttggata acttcatcaa cccgaaaaaa ccgtacccgg
     2641 ttatcgcgac cacttcagag ctgatgacca ccggtgtgga tgctaaaacc tgcaaactgg
     2701 tagtactgga ccagaacatc cagtcgatga ccaaattcaa gcagattatc ggtcgtggta
     2761 cacgcatcga cgaacgttac ggcaaactct ggtttaccat cctcgacttt aaaaaagcca
     2821 ccgaactgtt tgccgatgag cgtttcgatg gcattcccga aaaagtcatg gataccacac
     2881 cagaggatat cgccgatcca gaatctgatt ttgaagagaa actcgaagaa atcagcgaac
     2941 atgacgacga acaggtaaca ggcgttgatg aaccgcctgc gccaccatac caggttaaag
     3001 ataccgatga tgtcggccca cttccggaag aagacgagaa gaaaatccgc aagtttcacg
     3061 tcaacggtgt agcagtgggc gttattgccc agcgtgttca gtattacgac gccgacggta
     3121 aactggttac cgaatccttt aaagattaca cccgcaaaac actgctcaaa gaatatgcct
     3181 cgctggatga ctttacccgc aagtggcagg acgccgatcg caaagaagcg atcattcacg
     3241 agctggagca acaggggatc atctgggaag tactggcaga agaagtcggt aaagatctcg
     3301 acccgttcga catgctttgc cacgtagtgt atggtcagcc gccgttaacc cgcaaagagc
     3361 gcgccgagaa cgtgcgcaag cggaactact tcacaaaata ctctgaagca gcgcaagccg
     3421 tgctcgataa tctgctggat aaatacgccg atgcgggcgt acaggagatc gaaagtattc
     3481 aggtgctgaa acttaagcca ttcgacagca tgggcacctt accggagatt attaaaaccg
     3541 gatttggcga ccgtaacggg tataatcagg cgctcagcga gctggaaaac gaaatctacc
     3601 aattaccgcc ccgctctgct taacaactct cttttgcgcc ttctgcaacc atcatgttgc
     3661 agaaggcctt gtttttcatg gaatacaaca tgtctatcag ctcagtaatc aaatcattac
     3721 aagatattat gcgcaaagat gccggtgtgg atggcgatgc gcagcgtctc ggtcagctct
     3781 cctggctgct gtttttgaaa atcttcgatg cccaggaaga ggcgctggaa ctggagcagg
     3841 ataactatca atatccgatc ccacagcgtt atttatggcg cagttgggcc gccaacgcgc
     3901 agggcattac cggtgattcc ctgctggaat tcgttaatga tgatctgttc ccggcgttga
     3961 aaaacctcac tgcgcctatc gataaaaacc cacgcggcta cgtggtgaag caggcgttca
     4021 gcgatgccta taactatatg aaaaacggta cgctactgcg tcaggtgatc aataagctga
     4081 acgaaattga ctttaccagc gccagcgaac gccatctgtt tggtgatatt tacgaacaga
     4141 tccttaaaga tctgcaatct gcgggcaatg cgggcgaatt ctatactcca cgcgccgtca
     4201 ctcgctttat ggtggatcgc gttgatccga aactcggcga atccattatg gacccggcct
     4261 gcggtacggg cggttttctt gcctgcgcat ttgatcatgt aaagaacaaa tacgtgaaga
     4321 gcgtcgccga tcatcagacg ctgcaacaac agatccacgg tgttgagaaa aaacagcttc
     4381 cgcacctgct ggcgaccacc aatatgctgc tacacggcat tgaagtgcca gtgcaaattc
     4441 gtcacgacaa cactctgaac aaaccgcttt cctcccggga tgagcaactg gatgtcattg
     4501 ttaccaaccc gccgtttggt ggcacggaag aagacggtat tgagaagaac tttccggcag
     4561 agatgcaaac ccgcgaaacg gcggatttgt tcctgcaact gattgtggaa gtactggcga
     4621 aaaatggtcg cgcggcggtg gtattgccgg atggcacact atttggcgaa ggcgttaaaa
     4681 ccaaaatcaa aaagctactt accgaagagt gcaacctgca taccatcgtg cgcttaccga
     4741 atggtgtgtt taacccctat accggcatta aaaccaacct gttgttcttt accaaaggtc
     4801 agccaaccaa agagatttgg ttctatgagc atccgtatcc ggcgggcgta aaaaactaca
     4861 gcaaaactaa gccgatgaag tttgaagagt ttcaggcgga gatcgactgg tggggtaacg
     4921 aggccgatgg ttttgccagc cgcgtagaga atgagcaggc gtggaaagtc agcattgatg
     4981 acgtgattgc gcgtaacttc aatctggata ttaaaaaccc acatcaggcg gaaaccgtca
     5041 gccatgatcc ggccgagctg ttagcgcagt atgcaaaaca gcaggcggag atccagacgc
     5101 tgcgtaatca actgcgcgat attcttggcg ctgcgctgtc tggcaaggag gttaactaat
     5161 gagtgtggaa aagctgatcg ttgatcatat gaaaacctgg acctcggcgt tgcaaacccg
     5221 ttccaccgcc gggcgcggca gttccggaaa aattgattta tatggcatta agaaattacg
     5281 tgagctgatt ctggaactgg ctgtgcgcgg taaactggtg ccgcaggacc cgaacgatga
     5341 accggcgtcg gagctgctga agcgtattgc ggcagaaaaa gcagagctgg tgaagcaagg
     5401 taaaattaaa aagcaaaaac cactgccgga aattagtgag gaagagaagc cgtttgaatt
     5461 gccggaggga tgggagtggg tgcgtattag tgaaattgga catgactggg gacaaaaaac
     5521 tccagataaa gattttactt acattgatgt tggttcaata aataaagaat atggaattat
     5581 tgaagagcta tctattcttt cagcgaaaga tgccccatcg cgagcgagaa aaattgttca
     5641 gcaagggact atcatatact ctactgttcg cccgtatttg ttaaatatag ctattattga
     5701 aaacgaaata ctacctgaac cgatcgctag tactgccttc gctattattc atccatatac
     5761 ggcgatggat gctaatttca tttattatta tctacgttct ccggtatttg tttgttatgt
     5821 tgaaaattgt cagacagggg ttgcctatcc agcaatcaat gacaaacaat ttttttctgg
     5881 aataactcca gttcctccat ccttggaaca agttcgcatc gcaaacaaaa tcaaagaatt
     5941 aatgtccctc tgcgaccaac tggaacagca atccctgacc agtctggacg cacatcaaca
     6001 actggttgaa accctgttgg gaacgcttac agacagccaa accgccgagg aactggctga
     6061 aaactgggcg cgtattagcg agtatttcga cactctattt accaccgaag ccagcgtgga
     6121 tgcgttaaaa cagaccattc tgcaactggc cgtaatgggc aaacttgtgc cgcaggatcc
     6181 gaatgatgaa ccagcctctg aactgctcaa acgaattgcg caggaaaaag ctcaactggt
     6241 gaaagaagga aaaataaaaa aacaaaaacc gttgccgcca attagcgatg aggaaaaacc
     6301 gtttgaactt ccggaagggt gggagtggtg tttatttgaa gatattattg atattcaaag
     6361 tggtatcact aaaggaagaa atttatcaaa tagaactttg gtaaaagttc cttatttacg
     6421 tgttgcaaac gtccaacgcg gatatcttga tcttacggaa attaaacaga ttgaaatccc
     6481 tattgaggaa aaagaaaaat atcaagtagt caagggagat ttattgataa cagaaggcgg
     6541 cgactgggat acagtcggga gaactacagt atggtgtcat gactggtata tagcaaatca
     6601 aaaccatgta ttcaaaggac gaaatatagg gcaagatgtt gatccatatt ggttagaaac
     6661 atatatgaat agcccattct caagacaata ttttgctaac gcaagtaagc aaaccactaa
     6721 tttagcttct attaataaaa cccagctcag aggttgtcct gttgctattc ctcctagctc
     6781 agaagcaaaa aaaataatga gtaaactaca tattttttat aaactatgtg aagaattaaa
     6841 gaatcatatc caatccgccc agcaaaccca actacacctt gcagatgcac tcactgacgc
     6901 ggcggtaaac taatttacct gccctcgaaa aaccaataaa gcggctaaat agccgcttta
     6961 ttcacatcac caaaatttat atttcacgct aattttcatc ttattttcat taatttttag
     7021 ctggactttc cccatattta ctgatgatat atccaggtat ttagcgcggt gcggatgtgc
     7081 gccaacacac ccgcaccgct aatcacaatc actatttata gggaatagca gttatgactg
     7141 atacgcattc tattgcacaa ccgttcgaag cagaagtctc cccggcaaat aaccgtcaat
     7201 taaccgtgag ttatgcgagt cgttacccgg attacagccg tattcccgcc atcaccctga
     7261 aaggtcagtg gctggaagcc gccggttttg ccaccggcac ggtggtagat gtcaaagtga
     7321 tggaaggctg tattgtcctc accgcccaac cacccgccgc cgccgagagc gaactgatgc
     7381 agtcgctgcg ccaggtgtgc aagctgtcgg cgcgtaaaca aaggcaggtg caggagttta
     7441 ttggggtgat tgcgggtaaa cagaaagttg cctgatacat tgacccggta gcgccaggac
     7501 taccgggcat ttttcgctgc gttataccaa cgcgctctcc atctgttttt caaatgcctg
     7561 acgaattaac cccataatgt aaggaagttg tgcgaggtca ctgaaaccaa tttccacatc
     7621 accattgccc catcgcccaa catttgtcac atctttggca atacctttcg gatcgaccag
     7681 ttcatgaaac tgcatattaa gcgacaatcg cagtcggctt ttttgcggca caacatcaac
     7741 aaaattcgtt tccgctttaa acgcaatata gagcttcaat acttcctgcg tgatccctgc
     7801 gtccaggcgc ataatttcat cgcgaagatg atcgaataac acccggctat gcgaaccatc
     7861 agcaagaaaa ggataatcac tcaggctgta actggttttg ttctcaggct gtccccgata
     7921 aacagctaaa acctcttcag gaagagaagg cgcttgccag acctgaaccg caagctccgc
     7981 cagacgatcg gctcgggcgt gaatggcagc ttcatcccat ttttcgcact gtccaagacc
     8041 gatattcaaa tagagcgggc tatgcttaaa gccgtcttca atatcgcgtt tttctgcgaa
     8101 aggtctgtca ctgtaacggg agttatagcg cgttaaagtg agattcccca acgtatgcaa
     8161 cagttcttta tgaatacgct gccagtcact tcccagctct tcgcgccatt tagccgacaa
     8221 attttcgttc tgcggcataa tatgttcaat cgtaaactct tccacgcgct ctctgcgttt
     8281 atcgttttcc agtcgtcgta accagtagct acgactacgg aagttataga gatcgcgaac
     8341 ttttaattcc cgtttaaatt catcatcgtt ggggaaacga cgatatgaag gtagattcaa
     8401 aaaatgtacc tgaatacttt ccagatattt ttctttatta atgaccttat aaaaagtagc
     8461 aaacgtcttg ttaagagaat ttgtcggaat cgcacatact gcacggcgga aaacataaga
     8521 ttcaattaaa cgaattatgc tcaggaaatc ttcgtgagac aaatcgtcat ttttatagtc
     8581 atgataaagc gccagtaaga aaggatacgc cacatcaacc tttaactcgc gcaaatcctg
     8641 aaaagccgta gcaagcgatt tgtcactttc ttttcccaat gccattgcac agtaatactc
     8701 cgcgtagata tgaatatctt caaccagccg atctacgcct ttttcagcaa cactctggct
     8761 gcgggcatgg agtttaaatg cctcatagac ttcatctgtc cgagggatct ccccgttttt
     8821 accgtcagat aatgacgcat aaaactgtca aaatattcgc tgtaaccttg ctgaccacaa
     8881 gcgacttcca ttggacgcca gtgatcttca tacaaccggg tttgatgctc tggttcgagg
     8941 cccatcagaa taaaattgcg caccagatcg gcctgactta acgccttacc ggtggagttc
     9001 atactttcaa aaatcagttg cggattatcc tgaccacgat taagcgccac atcgacaatt
     9061 aataactttg ctaacccacg acataagggg atcaagtcat caccgagttt ggcaatttgt
     9121 tcatcaaaga aagtaaagtt ttccattatt cgatgggaat agttttctgg cattggtctt
     9181 tgttttatta acgccagtaa actgtcttta tcagtctcgg tcagcagtaa tttaaaacct
     9241 ttctcgccgg actcataagg attgagcaaa taataattac gcaatttcat tgccgaaaag
     9301 ccatcaaaaa cttcgtcttc gccaagatgg cgcgataaag cctcaatcag caacattgcg
     9361 gtcgtcagcc gctgctgacc atcaatgacc agcaacggag aaataccaga aacctgatac
     9421 aatccctgct ctatataaac aaccgaaccg ataaaatgcg ctgatatatc gtcacgcttt
     9481 cctgcacgaa tgatgtcgtc ccaaagttgc cgacattgtt gttctgtcca tgaataagtc
     9541 cgttgataaa tgggaataac aaactgctgc gaacgtttta aaaaatcgag taggcgagct
     9601 tctgtagctt tcattctgac gtctttccgt gaaacgatga tttcgcaata ataaccactt
     9661 ccttatggag gttatagaga aatcctgttt atccgcgacg aacatacccc tcactccaca
     9721 tcctccctgc gtaacccaat atccctcaac cgttcatcac tcatctgctg taacacccgt
     9781 cgggcctgca tttgccgccg ccaacgcctc actgcctgcc agagttgcac aagtctgata
     9841 aacggcgttt tggctttgtt ttcgtgaaat tccatctgct gcctcctcat atttcagagg
     9901 cttcatcatc cgtcttctgg cagttcacaa tacagatgca aatattagtt ttgtttaaca
     9961 tacagacagg ttaaaacagc atctgcatcc ggttttttag cctcatctgt accggttttt
    10021 ctttctgtat ggtccaaata aggaatacag catgacgcgt tatcaacatc tggcgactct
    10081 acttgccgag cggattgagc aagggctgta tcgtcacggg gagaaattgc cgtcggtgcg
    10141 cagcttaagt caggagcacg gcgtcagcat cagcaccgtg cagcaggcgt accagacgct
    10201 ggagacgatg aagctcatca ctccgcagcc gcgttcgggt tattttgtcg cacaacgtaa
    10261 agcccagccg ccagtgccgc cgatgacgcg tccggtgcag cgcccagtgg aaattaccca
    10321 gtgggatctg gtgctggata tgctggtggc gcatagcgac agttccattg ttccgttaag
    10381 caaaagcacg ccggatgtcg aaacgcccag cctgaaaccg ctgtggcggg agctaagccg
    10441 ggtggtgcag cataatctgc aaaccgttct cggttatgac ttgctagccg gtcagcgggt
    10501 attgcgcgag caggttgccc gcctgatgct cgacagcggc tcggtggtca ctgccgatga
    10561 catcatcatc accagcggct gccataactc gatgtcgcta gcgttaatgg cggtgtgtaa
    10621 accgggcgat attgtcgcgg tcgaatcccc ctgttattac ggttcaatgc agatgctgcg
    10681 cggcatgggc gtgaaagtga ttgaaatccc aaccgatcca gaaacaggca tcagcgttga
    10741 agcgctggaa ctggcgctgg aacagtggcc gattaaaggc atcattctgg tgccaaactg
    10801 taataatccg ctgggattta ttatgccgga cgcgcgcaaa cgggccgttc tctctctcgc
    10861 tcagcgtcat gatattgtga tttttgaaga tgatgtctac ggcgaactgg caacggagta
    10921 tccgcgcccg cggaccattc attcctggga tatcgacggg cgagtgctgt tgtgcagctc
    10981 gttcagtaaa agtattgctc caggcctgcg cgtgggttgg gtcgcaccgg ggcgttatca
    11041 cgataaactg atgcatatga aatacgccat cagcagcttt aatgtgccgt ccacgcaaat
    11101 ggcggcggca acgtttgtgc tggaaggtca ctatcatcgc catatccggc ggatgcggca
    11161 gatctatcag cgcaatttgg cgctttatac ctgctggata cgggaatatt ttccctgcga
    11221 aatctgtatt acgcgcccga aaggcggatt tttactgtgg atagaattgc ctgaacaggt
    11281 cgatatggtc tgcgtcacgc ggcagctgtg ccgcatgaaa atccaggtgg cggcaggctc
    11341 gattttctca gcttccggca aataccgtaa ctgcctgcgc atcaactgtg ctttgccact
    11401 cagcgaaact tatcgcgaag cgctgaagca aattggcgag gccgtgtatc gggcaatgga
    11461 ataaattaga attagtggcg aagcacctga tttattctca cgaatcgcct gtgactcgtt
    11521 ttatctttgc aggatcatag ccacgttccg gcatggctta ggtaaagtgc gctttgttca
    11581 tgccggatgc catgtgaacg ccttaccagg tctacaaaat ctttaaagtt caatatattg
    11641 aatgggcttt gtagacctga taagcgtagc acatcaggct gctttgcgtt tgtcatccgt
    11701 ctctggttag ctcttgagcc atgaatagcg ctcacgctca ggcaagggct gccttaatgc
    11761 ctgcatttgc tctgccgaaa gtccggtaat cttctgtatc cattcaggat ctgccccatt
    11821 ctgcaacaac aatcgaagaa tgcgctgttc cccttcttcc cttcccaaca gccatccatc
    11881 attatgaatt cgctctgcaa tcgtcattat gaattcgctc tgcaatcgtc attattcgct
    11941 ctctgtgttg aggcattcga ctggttagtt cactgataaa ctcattaaaa cgctcttcat
    12001 cgccagtcag taaaatgtaa tttaacagcg cagttatctg gctgtcatta gcacactctg
    12061 taaccagtaa tactaccagt tgatcgataa gccccatcag atcgcgctgg cgaatatgct
    12121 tttggatcaa ctccaacagg gcgactctgc ggtgctgcac aatctcgtcg tccggcacga
    12181 cagtgatatc caccagcggg aacgcggcgt tataaagctt ccgtgcagta gtcgggtcgg
    12241 caaattcgtc caaccagcac aaagaccagg gataaggact acggctaccg tgataaaaca
    12301 gcatcgggat gaccagcggt agtggccggc gtttatcatg ctctatatgg cgctgcatca
    12361 ctgccatgga atagcgcatc aggcgaaagg ccatatgaat atcctcacgg ctctgatgtt
    12421 caatcaccac atagatatag ccatcgcctt cccgggtctt taccgaccac agaatatcgg
    12481 agtgtagcgc ccgcaatttt tcatcgacaa agctggcgga ttccagtttt aagctgtcga
    12541 gatcgcacag ttcacgtaaa tctttgggta agtggatctc cataaaatcc cgcgcggtgt
    12601 cagggtgcat gagaaaagat ttaaataatg cgtcatgcgg cgtgctggtc gtgaagtttg
    12661 tcatggcgat ccgtcacctg ttgataagat ggcccgactt tagcgactaa caaaaccatt
    12721 ctcacccggc aatttctcgt ttcccgaggc gctttccagg ctatttttgt tcgtctgcaa
    12781 acgatacatt tttccggaaa ctgactcgcg ttttacatgt cctgtacccg gcagatattc
    12841 ccccttccac cgcgctcttt tgattaatcg caaattttaa ttacacttaa ggtgtatatt
    12901 ttctatgcaa cccatcaatt caagaggtgt aatgtgctga tgactatttg taatcgttat
    12961 acattctgac ccgaagtcag aaggtatttc tctgtctgtg tgttcacagg cagtgtggtt
    13021 gattacatga aattcagtac atttgtcctt ctcgcctacc tttcgtcatt accgacggta
    13081 ttgaatttcg ttttccccgt tggggttctc cggacaagga gttgtttgtt atgccacgtt
    13141 tttttgcccg ccatgccgcc acgctgtttt tcccgatggc gttgattttg tatgactttg
    13201 ccgcctatct ttcgacggac ctgatccagc ctgggatcat taatgtggtc cgtgatttta
    13261 atgccgatgt cagtctggct ccggctgccg tcagtctcta tctcgctgga ggcatggcgt
    13321 tgcagtggct gctggggccg ctttccgaca ggattggccg caagccagtg ctgattaccg
    13381 gggcgctaat atttaccctt gcctgcgctg cgacaatgtt cacaacgtct atgacacagt
    13441 ttcttatcgc gcgtgcaatt cagggcacca gtatctgttt tattgccacc gttggttatg
    13501 tcacggtgca ggaggcgttc ggccagacaa aagggatcaa gttgatggcg attatcacct
    13561 ccatcgtact gattgcgccg attatcggcc cgctttccgg cgcagctctg atgcactttg
    13621 tgcactggaa agtccttttt gccatcattg cggttatggg ttttatctca tttgttggct
    13681 tactgctggc gatgccagag acggtgaagc gcggcgcggt tccatttagc gccaaaagcg
    13741 tcttgcgcga ttttcgtaat gtcttttgca atcggctgtt cctctttggc gcagcaacca
    13801 tctctttaag ctatatcccc atgatgagtt gggtggctgt ctcgccggtg atcctgattg
    13861 acgcaggcgg cttaacgacc tcgcagttcg cctggacaca ggttccggtg ttcggcgcgg
    13921 tgattgtcgc gaatgccatc gtggcgcgtt tcgttaaaga tccgaccgaa ccgcgcttta
    13981 tctggcgtgc cgtacccatt caactggtcg gcctcgcgct gttgattatc ggcaatctgc
    14041 tgtcaccgca cgtctggctg tggtcggtgc tgggcaccag tctgtatgct ttcgggattg
    14101 gtttgatctt cccgacctta ttccgcttta cgctgttttc caataactta ccgaaaggga
    14161 ccgtctccgc atcgctgaat atggtgatcc tgatggtgat gtctgtctcg gtcgaaattg
    14221 gccgctggct gtggtttaac ggcggtcgct tgccgtttca tctgttagcc gttgtggcgg
    14281 gcgttatcgt cgttttcacc ctggccggat tgcttaatcg cgtgcgccaa catcaggctg
    14341 ccgagctggc agaggagcag tgatttttgc gcgatcctgc cgtcaggctc tattcttaac
    14401 gttatgaata aactcattga actcagacgc gccaaaatgt tggcgctctc tttactgctt
    14461 atcgccgctg ctacctttgt cgttacgctg tttttgccgc ccaatttttg ggtgagcggc
    14521 gtgaaggcga ttgctgaagc ggcgatggtc ggcgcgctgg cggactggtt tgcggtggtg
    14581 gcgctgtttc gccgcgtgcc gattccgatc atttctcgtc atacggcgat tatcccgcgt
    14641 aataaagacc ggattggcga aaatctcggt cagttcgtgc agaaaaaatt tctcgatacc
    14701 caatcgctgg tggcattgat tcgacgtcac gaaccggcgt tgctgattgg caactggttt
    14761 agccagccag aaaacgcccg ccgcgttggt cagcatctgt tgcagatcat gagtggtttt
    14821 cttgaactga ccgatgatgc acgtattcag cgcctgctta agcgcgcggt ccatcgggcg
    14881 attgataagg tcgatctttc cggcaccagt gcgttgatgc tggagagtat gaccaaaaac
    14941 gatcgtcatc aggtgctgct ggatacgctg atcgcacagt tgatcgccct tctccagcgc
    15001 gataaatcgc gcaagtttat cgcccagcag attgttcgct ggctggagag tgagcatcca
    15061 ctgaaagcca aaattctccc caccgaatgg ctgggcgaac atagcgcgga gttggtttct
    15121 gacgcggtga attctttgct tgatgatatc agccgcgatc gtgcgcatca gatccgccat
    15181 gcatttgatc gcgccacttt tgccctgatc gacaagttga aaaacgatcc tgaaatgaca
    15241 gcgcgagctg atgccgtaaa aagctatctg aaagaggatg aagcctttct cagtgaattg
    15301 tggggggatt tacgggaatg gctgaaagcg gatatcaaca gtgaagattc tcgtgtgaaa
    15361 gaacgtatcg cgcgggcagg tcaatggttt ggcgaaacgt taattgccga tgatgccttg
    15421 cgggcgtcgt taaatggtca cctggaacaa gccgcgcacc gcgtcgcgcc tgagttttct
    15481 gcattcctga cgcgccatat cagcgatacg gtaaaaagct gggatgcccg ggatatgtcg
    15541 cggcaaatag agttaaatat cggcaaagat ctgcagttta tccgtgtcaa cggtacgctg
    15601 gttggcggtt gtattgggct aattttatat ttgctgtcac agctcccggc cttgttcccc
    15661 ctcggcaatt tttagaaatc catgaaaaac taagtggcta accgcgagag agatcaaata
    15721 aaccatacta aaagaatggt aataatgtgg cctcttttca ttatctcccg tggtacgggg
    15781 aaggaaaatc atgtcacttg tcaccgatct acccgccatt ttcgatcagt tctctgaagc
    15841 tcgccagaaa ggctttctca ccgtcatgga tctcaaggtg cgcggcattc cgctggttgg
    15901 cacttactgc acctttatgc cgcaagagat cccgatggca gccggtgcgg ttgtggtttc
    15961 gctctgttcc acctctgatg aaaccattga agaagcggag aaagatctgc cgcgcaacct
    16021 ctgcccgctg attaaaagta gctacggctt cggcaaaacc gataaatgcc cctacttcta
    16081 cttttcggat ctggtggtcg gtgaaaccac ctgcgacggc aaaaagaaaa tgtatgaata
    16141 catggcggag tttaagcccg ttcatgtgat gcagctgcca aacagcgtta aagacgatgc
    16201 ctcgcgtgcg ttatggaaag ccgagatgct gcgcttacaa aaagcggtgg aagaacgttt
    16261 tgggcacgaa attagcgaag atgctctgcg cgatgccatt gcgctgaaaa accgcgaacg
    16321 tcgcgcactg gccaattttt atcatcttgg gcagttcaat cctccggcgc ttagcggcag
    16381 cgacattctg aaagtggttt acggcgcaac cttccggttc gataaagagg cgttgatcaa
    16441 tgaactggac gcgatgaccg cccgcattcg tcagcagtgg gaagaaggcc agcgactgga
    16501 cccgcgtccg cgcattttaa tcaccggctg cccgattggc ggcgcagcag agaaagtggt
    16561 gcgcgcgatt gaagagaatg gcggctgggt tgtcggttat gaaaactgca ccggggcgaa
    16621 agcgaccgag caatgcgtgg cagaaacggg cgatgtctac gacgcgctgg cggataaata
    16681 tctggcgatt ggctgctcct gtgtttcgcc gaacgatcag cgcctgaaaa tgctcagcca
    16741 gatggtggaa gaatatcagg tcgatggcgt agttgatgtg attttgcagg cgtgccatac
    16801 ctacgcggtg gaatcgctgg caattaaacg tcatgtgcgt cagcagcaca acattcctta
    16861 tatcgctatt gaaacagact actccacctc ggatgttggg cagctcagta cccgtgtcgc
    16921 ggcctttatt gagatgctgt aaggagtagc agtgacatat tcgattggca ttgattctgg
    16981 ttctaccgcc accaaaggga tcttactcgc agacggcgtg attacgcgcc gtttcctcgt
    17041 tccaacaccc tttcgaccgg caacagcaat tactgaagcc tgggaaactc tgcgcgaagg
    17101 gttagagaca acgccgtttc tgacgctcac cggctacggg cggcaactgg tggattttgc
    17161 cgataaacaa gtgacggaaa tctcctgtca cgggctgggc gcacggtttc ttgcgccagc
    17221 aacgcgcgcg gtaatcgaca tcggtggtca ggacagcaaa gtgattcagc ttgatgatga
    17281 cggtaacctg tgcgatttcc tgatgaatga caaatgcgcg gcgggcaccg ggcgtttcct
    17341 ggaggtgatc tcgcgcacgc ttggcaccag cgtcgagcaa ctcgactgca ttaccgaaaa
    17401 tgtcacgccg cacgccatca cgagtatgtg cacagtgttt gctgaatcag aagtgatcag
    17461 cctgcgctca gcgggcgtcg cgccagaagc gattctcgcc ggagtgatta acgcgatggc
    17521 gcggcggagt gccaatttca ttgctcgtct ctcctgtgaa gcgccgattc tgtttactgg
    17581 tggcgttagt cattgccaga cgtttgcccg gatgctggaa tctcacctgc gaatgccggt
    17641 aaatacccat cctgatgcgc aatttgctgg cacaattggc gcggcggtaa ttggtcaacg
    17701 agtgaggaca cgccgatgaa agagttttta tttttatttc actccacggt cggcgtcata
    17761 caaacccgca aggcgttgca ggcagcgggc atgacttttc gcgtcagcga tattccccgt
    17821 gatttacgcg gcggctgcgg gttatgcatt tggctgacct gtccccccgg cgaggaaata
    17881 caatgggtga tccctgggca aaccgaagcg atatattgcc agcagggcag cgactggcag
    17941 tgcgtggtgc attacgatgc agaagcgtca accaggcaat aggatcttta aatataccaa
    18001 ccggataatt ggctgcgcat aatcctgcat gaaggaagtt gtagtaaata tctcgcctgc
    18061 cgtgaaatat ttcccatcca caaaatgagc gatttttgat agcaccttcc tattgcgcgt
    18121 ctaaaataca atatacattg aattatactt aaacccataa tcatgcatag agaaataaaa
    18181 tgattaccgg gtgttgtaat gttaaaagat aagtttctgt gcaaagctta tctttcccca
    18241 ccccctgatt attttagatt tagaagaata tttcacagtt caaggataaa aatatgggct
    18301 ccctatttaa catatacaaa gatattttcc caacactcgg catgtattcg ggactaaaag
    18361 cttgccatga aaaaaacaac ctaccatttg atattaacac ggaaattgaa accatacaaa
    18421 aacaaattaa ttatgatata aatcatttga atgatggttt gattaagcgt gtactgaatc
    18481 tttttattca ccttatctct aatcccgaca atcttg
//
LOCUS       DAZFAB010000091        18369 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZFAB010000091 DAZFAB010000000
VERSION     DAZFAB010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.091"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..401)
                     /locus_tag="ACHJWA_003542"
     CDS             complement(<1..401)
                     /locus_tag="ACHJWA_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4310066.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGR"
     gene            complement(503..1483)
                     /locus_tag="ACHJWA_003543"
     CDS             complement(503..1483)
                     /locus_tag="ACHJWA_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFO4310067.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVREAYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKELLTHLICLGDEEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPEKHLLDERLRKEKEDAINQINHVRNVLQQIKQEASHLLNH"
     gene            complement(1491..2201)
                     /locus_tag="ACHJWA_003544"
     CDS             complement(1491..2201)
                     /locus_tag="ACHJWA_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO4310068.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(2418..4241)
                     /gene="typA"
                     /locus_tag="ACHJWA_003545"
     CDS             complement(2418..4241)
                     /gene="typA"
                     /locus_tag="ACHJWA_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFO4310069.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            4614..6023
                     /gene="glnA"
                     /locus_tag="ACHJWA_003546"
     CDS             4614..6023
                     /gene="glnA"
                     /locus_tag="ACHJWA_003546"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFO4310070.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            6197..7246
                     /gene="glnL"
                     /locus_tag="ACHJWA_003547"
     CDS             6197..7246
                     /gene="glnL"
                     /locus_tag="ACHJWA_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFO4310071.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            7258..8667
                     /gene="glnG"
                     /locus_tag="ACHJWA_003548"
     CDS             7258..8667
                     /gene="glnG"
                     /locus_tag="ACHJWA_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFO4310072.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            8779..8889
                     /locus_tag="ACHJWA_003549"
     CDS             8779..8889
                     /locus_tag="ACHJWA_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFO4310073.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(9079..10452)
                     /gene="hemN"
                     /locus_tag="ACHJWA_003550"
     CDS             complement(9079..10452)
                     /gene="hemN"
                     /locus_tag="ACHJWA_003550"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFO4310074.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(10641..11147)
                     /gene="yihI"
                     /locus_tag="ACHJWA_003551"
     CDS             complement(10641..11147)
                     /gene="yihI"
                     /locus_tag="ACHJWA_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFO4310075.1"
                     /translation="MKPSSPSRSKGHAKARRKTREELNQEARDRKRQKKRRGHAPGSR
                     AAGGNNTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELLE
                     TDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQED
                     MMRLLRGN"
     gene            complement(11266..11432)
                     /locus_tag="ACHJWA_003552"
                     /pseudo
     CDS             complement(11266..11432)
                     /locus_tag="ACHJWA_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11729..12361
                     /gene="yihA"
                     /locus_tag="ACHJWA_003553"
     CDS             11729..12361
                     /gene="yihA"
                     /locus_tag="ACHJWA_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFO4310076.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFNEMQPVEETQDGE"
     gene            complement(12743..15529)
                     /gene="polA"
                     /locus_tag="ACHJWA_003554"
     CDS             complement(12743..15529)
                     /gene="polA"
                     /locus_tag="ACHJWA_003554"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFO4310077.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHRDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            15893..16825
                     /locus_tag="ACHJWA_003555"
     CDS             15893..16825
                     /locus_tag="ACHJWA_003555"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFO4310078.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
     gene            complement(16866..18296)
                     /locus_tag="ACHJWA_003556"
     CDS             complement(16866..18296)
                     /locus_tag="ACHJWA_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFO4310079.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYQNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDSSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQQSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT         4427 a         4645 c         4436 g         4861 t
ORIGIN      
        1 aaccgacctt gttgttcgct attgcccaat cgacttaccg acttgagcaa gaccggccag
       61 ttcatcatgt cgcaagtaac gccaaatagc gcccagacaa agaggatgcc ccaatacccc
      121 ggcattgtcg tcaaataaac acccagtaat cctgtcgcaa tgagtgagaa tgacatggtg
      181 tatctgcgcg gtagtttatc ggcaaaataa atagacagaa aaaagcccac tgtggtaaca
      241 aatgagttta ccgacatagc attaccaatt tgaccattgg tcaaatggaa atattcctgc
      301 atcgggatat aaaacgcatc tttcagcgac ggtaatttat aaattgtacc gccacacagt
      361 gttaatagac taaataacgc ccatttcttt ttcgtgagca taacatcccc taagcgattt
      421 taaaaatcac tgttttaaag gcaataagat accgaccatt cttatggaat ggttttatta
      481 actgcgtatg agttaaaact tgttagtggt tcagaagatg gctcgcctct tgtttgattt
      541 gttgtagtac gttacgcaca tgatttatct gattaattgc atcttctttt tctttacgta
      601 accgctcatc cagcaaatgc ttttctggca atggtgtttc actcatctgg cgataaggga
      661 tccacggatt atcatcttcg tcttcaaagc ggaacgctgg agcataataa tcaacctctt
      721 cttccaggcc atatgccgtc acctgcggtt cttcatcgcc gaggcagata aggtgtgtta
      781 ataactcttt gaacggcata tctccctgac ctgaaataca ggctttatga cccaggccac
      841 cttgttcttt aacgatcaag gcatctttga tatggacctg ggtaatatgc ggtgccatcg
      901 tttttaaggc gtctatggga tgctcatttg cattgatcat attcgcaaaa tcaaacagta
      961 aggaaagaga ctccatctca ctttctttga ccagcgacac caactcatga ctttttaaat
     1021 cttcatgctg ttcgatcgta aaagtcaggc cgctatcctg atacgcttcc cgcacatagg
     1081 caatgtcgtt agcgatgatc gataatacgt cgcgcaaatt accttcataa cgtggataaa
     1141 aacgtacgga cgatgcccca gttttcaacg cgatggcgac ggcttcgtca atagatgcct
     1201 tatctgaggc gctggtttca atatgaatat caaggttcag acggcgggct ttatccccaa
     1261 aggcagagag ttctttatcg tccatattac caagagaaaa acgctcgcca tccagaacat
     1321 ggattttgac gccacgtaga ttattttcac ttgcgatatc aagtaaatca gcaggcaaca
     1381 ctctttccag ccgcatattt aaatgaaagg cataagcatg taaataaaga ggaagagtgt
     1441 cgacacgttg tagaatcttt cttgcgttat ttattgtcac cataaaacac ctacagatta
     1501 atgatgaggt ctacggcctc aaatcgccaa tattcaatat ctatctggac aatattgcct
     1561 tgctggtcgg cgcgatgctt ttcaattaag atcgctggca tacccgcaga accgccaaga
     1621 tacttgctga tatcgcccgg cattctgacg ggtttgaaaa tcaatttttt tactaccgtc
     1681 tctttttggt agcacttttc ccagactgca gaaaatgagt ggttttcaag ttgttcaata
     1741 aaacccggag caacttcagg attaatatat gtttcgtgat agaaaacttt atgtccttcc
     1801 agcgcccccc agccggtgat gcgatagagt tcagttgatg gtgtcactgc aatcagtggc
     1861 gcgagcgtct cgggaatatc gctggtacgg tttttctcgg taaaccccca ggaaggctct
     1921 cgcccttgct caattgcggc ccgctgaaag ctcgccgaca gctccggact gtaattaaat
     1981 cgcggctggg tcacgaacca ccccttacga tccttacgga agattttgga ttctgcctcg
     2041 aggttcaaca acgcctggcg cagcgtcata cgtttaatgc ccagcagttc tcccagctct
     2101 ctttctgaag ggagttttcc acccggcgtg gtgatgccat ctttcaacca ccgatccagt
     2161 ttctcttttg cattttctac ggtggattga ttctccgcca ttgtttaatc cttttggttt
     2221 aaaccagatg aagcatctta cgcttacgcc aaaaaagaaa gcaattaacg gcgtatgact
     2281 ttgcagataa ttgtgacaaa gctctctatt gacgtaacca cagacttata agggagatac
     2341 tcgcattggt gtgcgctcac gacaggcaaa aaaaaacctg ccatcgctgg caggtttttt
     2401 atgactaaaa aacgaaatta atcgtctttc ggtgcgcggt tggcgcgacg acgatcgttt
     2461 tccgtcaggt gacgtttacg aatacggata gaggtcggag tcacttctac cagttcgtca
     2521 tcatcgatga actccagagc ttgttccaga gtcatgcgga taggcggaac cagaacaacg
     2581 gcttcgtcag taccggaagc acgcatgttg gtcagtttct taccggtcag gcagtttaca
     2641 gtcaggtcgt tagagcggct atgaataccg ataatctgac cttcgtaaac ttctgcaccg
     2701 tgaccgagga acagcttacc gcgatcctgc aaaccgaaca gcgcgaacgc gaccgcttta
     2761 ccctgaccgt tagagatcag tacgccgttc tgacgctgac ccacttcacc cggacgcacg
     2821 tcgtcgtagt ggctgaaggt ggagtacagc agacccgtac cggaagtcat ggtcatgaac
     2881 tcagaacgga agccaatcag accacggctt gggatcacgt agtcgagacg tacgcggcct
     2941 ttaccgtctg gattcatgtt tttcaggtcg cctttacgtt cgcccagcgc ctgcattaca
     3001 gaaccctgat gctgttcttc aacgtccaga gtcacgtttt catacggctc ttgtttacga
     3061 ccgtcgattt cacggaagat aactttcgga cgggataccg ccagttcgaa accttcacga
     3121 cgcatgtttt cgatcagaac agacaggtgc agttcgccac gaccagaaac gcggaacgca
     3181 tcggcgtctt cggtttcttc tacgcgcagc gcaacgttgt gtaccagttc tttgttcaga
     3241 cgatccagga tctgacgaga cgttacgaac ttaccttctt taccgcagaa cggcgaggtg
     3301 ttaacgcaga agaacataga aacggtcggc tcatcaacgg agagtgccgg cagcgcttca
     3361 acgttttgcg tgtcgcaaac ggtgtcagaa atgttcagtt cgcccaggcc cgtgatcgcc
     3421 acgatatcgc cagcttccgc cagatcggtt tcgatacgtt ccagaccgag gtggcccagc
     3481 actttaccaa ctttcgcgtt gcgggttttg ccttcgctat cgatgatagt aacctgctgg
     3541 ttcggcttca ctttaccgcg cttgatgcgg ccaatgccga taacgccaac atagctgttg
     3601 taatcgagct gagaaatctg catctggaac ggaccgtcaa ggtcaacgtc cggcgcagga
     3661 acgtggtcaa caatcgcctg gtacagcggg gtcatgtctt ccgccatatc ttcgtggtcc
     3721 agacccgcga taccgttcag cgcagaagcg taaacgatcg ggaagtccag ctgctcgtcg
     3781 gtcgcgtcga ggttaacgaa cagatcgaat acctgatcca caacccaatc aggacgcgcg
     3841 ccagggcggt caactttgtt gataacaaca atcggcttca ggccgtaagc aaacgctttt
     3901 ttggttacga agcgcgtttg cggcatcggg ccgtcaaatg cgtcaaccac cagcagcact
     3961 gagtctacca tggacattac acgttcaact tcaccaccga agtcggcgtg ccccggggta
     4021 tcaacgatgt tgatacggta atcattccat ttgatagcgg tgtttttcgc gaggatggta
     4081 atcccacgct ctttctccaa atcgttggag tccatcacgc gctcttgggt ttcggcacga
     4141 gagtcgaacg taccggattg ttggagcagc ttgtctacca gggtggtttt accatggtct
     4201 acgtgcgcga tgatggcgat attacgcaat ttttcgatca caactttgcc tcaggcatta
     4261 gaaatagcgc gttattgtac acggattaat cgcactacaa aacaggatca caaacatcct
     4321 ccgcaaacaa gtattgcaga gtccctttgt gatcgctttc acggagcata aaaagggtta
     4381 tccaaaggtc attgcaccaa tatggtgctt aatgtttcca ttgaagcact atattggtgc
     4441 aacattcaca tcatggtgca gcccttttgc acgatgttaa gcatgataac gccttttagg
     4501 ggcaatttaa aagttggcac agttttcgct ttatcttttt tacggcgaca cggccaaaat
     4561 aattgcagat ttcgttacca cgacgacaat gaccaatcca ggagagttaa agtatgtccg
     4621 ctgaacacgt actgacgatg ctgaacgagc acgaagtgaa gtttgttgat ttgcgcttca
     4681 ccgataccaa aggtaaagaa cagcacgtca ctatccctgc tcatcaggtg aatgctgaat
     4741 tcttcgaaga aggcaaaatg tttgacggct cctcgattgg cggctggaaa ggcattaacg
     4801 aatccgacat ggtgctgatg ccagacgcat ccaccgcagt gattgacccg ttcttcgccg
     4861 actccaccct gattatccgt tgcgacatcc ttgaacctgg caccctgcaa ggctatgacc
     4921 gtgacccgcg ctctattgcg aagcgcgccg aagattacct gcgttccact ggcattgccg
     4981 acaccgtact gttcgggcca gaacctgaat tcttcctgtt cgatgacatc cgtttcggat
     5041 catctatctc cggttcccac gttgctatcg acgatatcga aggcgcatgg aactcctcca
     5101 cccaatacga aggtggtaac aaaggtcacc gtccggcagt gaaaggcggt tacttcccgg
     5161 ttccgccggt agactctgct caggatattc gttctgaaat gtgtctggtg atggaacaga
     5221 tgggtctggt ggttgaagcc catcaccacg aagtagcgac tgctggtcag aacgaagtgg
     5281 ctacccgctt caataccatg accaaaaaag ctgacgaaat tcagatctac aaatatgttg
     5341 tgcacaacgt agcgcaccgc ttcggtaaaa ccgcgacctt tatgccaaaa ccgatgttcg
     5401 gtgataacgg ctccggtatg cactgccaca tgtcgctgtc taaaaacggc gttaacctgt
     5461 tcgcaggcga caaatacgca ggtctgtctg agcaggcgct gtactacatt ggcggcgtaa
     5521 tcaaacacgc taaagcgatt aacgccctgg caaacccgac caccaactct tataagcgtc
     5581 tggtcccggg ctacgaagct ccggtaatgc tggcttactc tgcacgcaac cgttctgcgt
     5641 ctatccgtat tccggtggtt tcttctccga aagcacgtcg tatcgaagta cgtttcccgg
     5701 atccggcggc taacccgtac ctgtgctttg ctgccctgct gatggccggt cttgatggca
     5761 tcaagaacaa gatccatccg ggcgaagcca tggacaaaaa cctgtatgac ctgccgccgg
     5821 aagaagcgaa agagatccca caggttgcag gctctctgga agaagcactg aacgagctgg
     5881 atctggaccg cgagttcctg aaagccggtg gcgtgttcac ggatgaagcg atcgatgcgt
     5941 acatcgctct gcgtcgcgaa gaagatgacc gcgtgcgtat gactccgcat ccggtagagt
     6001 ttgagctgta ctacagcgtc taatagttga agttgtacta cccggcgcaa caacgccggg
     6061 atttagttgc cgtggaaact ttcagcccat ctctgcatgg gcttttttct ccgtcaattc
     6121 tctgatgctt cgcgcttttt atccgtaaaa agctataatg cactaaaatg gtgcaacctg
     6181 ttcaggagac tgctttatgg caacaggcac gcagcccgat gctgggcaga tcctcaactc
     6241 gctgattaac agtattttgt taatcgatga caacctggcg atccattacg ccaaccccgc
     6301 cgcgcaacaa ctgctcgccc aaagctcccg caaattgttt ggtacgccgt taccggaact
     6361 gttgagctac ttctcattaa atattgagct gatgcaagaa agtctggagg cggggcaagg
     6421 ttttaccgat aacgaagtga cgctggtcat cgacggtcgc tcgcatatcc tttctgtgac
     6481 ggcccagcgt atgccggacg gcatgatcct gctggagatg gcgccgatgg ataaccagcg
     6541 ccgcttaagc caggaacagc tacaacatgc tcaacaagtt gctgcccgtg atttagtgcg
     6601 tggcctggca catgagatta aaaatccgct tggcggttta cgtggcgcgg cgcagctact
     6661 cagcaaagca ttacctgacc cgtcactgct ggaatatacc aaagtgatta tcgaacaggc
     6721 ggaccggctg cgaaatctgg tcgaccgtct gttggggccg cagctgcccg gtacgcgcgt
     6781 taccgaaagt attcacaaag tagctgaacg cgtggtaacg ctggtgtcga tggaactgcc
     6841 ggacaacgtg cggttgattc gtgattacga tcccagcctg ccggaactgg cgcacgaccc
     6901 ggatcaaatt gaacaggtct tgctgaatat tgtgcgcaat gcgctacagg cgctggggcc
     6961 ggaaggcggt gaaatcattc tgcgtacccg caccgcgttt caactgacct tacacggcga
     7021 acgctatcgg ctggcggcgc ggattgatgt ggaagataac gggccaggca ttccgcctca
     7081 tttgcaggat acgctgtttt acccgatggt cagcggccgc gaaggtggca ccgggcttgg
     7141 cttatccatc gctcgtaatt tgattgatca gcattcaggc aaaattgaat ttaccagttg
     7201 gccagggcat accgagttct cggtttacct gcctatcagg aaataaaggt gacgtttatg
     7261 caacgaggga tagtctgggt agtcgatgac gatagttcca tccgttgggt gcttgaacgt
     7321 gcgctcgctg gagcgggttt aacctgtaca acatttgaga acggcgcgga ggtactggag
     7381 gcgctggcga gcaaaacgcc ggatgtgctg ctttcagata tccgtatgcc gggaatggac
     7441 gggctggcac tgctcaagca gattaaacag cgccatccga tgcttccggt catcattatg
     7501 accgcacatt ccgatctgga tgctgccgtc agcgcctatc aacaaggggc gtttgattat
     7561 ctgcccaaac cgtttgatat cgacgaagcc gtggcgctgg ttgagcgcgc catcagtcat
     7621 taccaggaac agcagcagcc gcgtaatgtt cagcttaacg gcccaacgac cgatatcatc
     7681 ggcgaagcgc cagccatgca ggacgtgttc cgtattatcg gtcggctttc gcgttcttct
     7741 attagcgtgc tgattaacgg cgaatccggc accggtaaag aactggtcgc tcatgccctg
     7801 catcgccaca gtccgcgagc caaagcgcca tttatcgctc tgaatatggc ggctatcccg
     7861 aaggatttga tcgaatcaga actgtttggt cacgagaaag gcgcgtttac cggcgcgaat
     7921 accattcgtc aggggcgttt tgaacaggct gatggcggta cattatttct cgatgaaatt
     7981 ggcgatatgc cgctggatgt gcagacgcgt ttgctgcgcg tgctggcaga cggtcagttt
     8041 taccgcgttg gcggctatgc gccggtgaaa gtggatgtgc ggattatcgc tgccactcac
     8101 cagaatctcg aacagcgggt gcaggaaggt aagttccgtg aggatctgtt ccaccgcctg
     8161 aacgttatcc gcgttcatct gccgccgctg cgtgagcgtc gggaagatat tccccgcctg
     8221 gcgcgccatt ttttacaggt tgccgcgcga gaactgggcg tagaagcgaa gttgctgcat
     8281 ccggaaaccg aagccgcgtt gacgcgtctg gcgtggccag gcaacgtgcg ccagctggaa
     8341 aacacctgcc gctggctaac ggtaatggct gccggacagg aagtgttgat tcaggatttg
     8401 cctggcgaac tgtttgaatc aacggttgcg gagagtactt cgcaaatgca accggacagt
     8461 tgggcgacgc ttcttgcgca gtgggcagac agagcgctac gttccggtca tcaaaatctg
     8521 ctttccgaag cgcagccaga gctggagcgg acgttactga cgaccgcgtt gcgacatacg
     8581 caggggcata aacaggaggc ggcgcggcta ctcggctggg gccgcaacac cctgacgcgt
     8641 aagttaaaag agctggggat ggagtgattc acggcttgtg tgtaaagatt gattattgag
     8701 cgcaaattgc tggtattttg cgctttactg ttccgataag ttcagtatga tcttgcccgg
     8761 aaaacgggga gagtcattat gctggaatca ataattaatc tggtatcgag tggcgcagtt
     8821 gacagccaca caccgcaaac tgctgttgct gccgtgctgt gtgccgcgat gattgggctg
     8881 tttagttgag tttcagattg ctgatgaagt gctctttgtt catgccagat gctgcgtgaa
     8941 cgccttatcc ggcctacaaa agcaatattt tcaacaggtt ttgtaggcct gataagcgta
     9001 gcgcatcagg caatcttgtt ctcagcacat tgatggcgct ttcgttttct actttgtaaa
     9061 cgaagcgcca ttcactattt aaatcacccg agagaactgc tgcatccgcg ctttctggcg
     9121 cagataggta tcaaagcaca tgcaaatgtt gcggatcagc aagcgacctt tcgccgtcac
     9181 ctgtattccc ttctcatcca catccaccag cccatctttt gctaacgggg cgagcagctt
     9241 gagatcttcc gcaaagtaat cagcgaagtg caaatcccac tgtttctcaa taggggcgta
     9301 atccagacgg aagttgcaga tgagcgactt aatcacatcg cggcgaatac agtcatcacg
     9361 cgttagcgca ataccacgcc acagcgcatt gccttgttca tccacttgct gatagtactg
     9421 cttcaactct ttctggttct gcgcgtagca gtcgccaatc atgctgatgg cggaaacgcc
     9481 catccccagc agatcggtat cgccctgagt ggtgtagccc tggaaattac gatgcagcac
     9541 gccttcacgc tgggccaccg ccagctcgtc atccggacgg gcaaagtgat ccataccgat
     9601 aaactgatag cccgattgcg tcaggaaggc gatggtttcc tgcaggatat cgagtttttg
     9661 ctgcggactc ggcaggtcag catctttgat tttgcgctga gcagcaaaaa tggtcggcag
     9721 atgcgcatag ttaaagacgc tcagacggtc agggtttagc tccgccacgc gtttcagggt
     9781 aaaggcgaaa ctttccggcg tctgtttcgg caggccgtaa atcaggtcga tgttggtgga
     9841 ggtaaagcca atctcacgcg catggttaag cagcgcaaag ataaactctt catcctgctc
     9901 gcggttaacc agacgttgca cttctttgtt gaagtcctgc acgcccatac tcaggcgatt
     9961 aaagccctcg gcgcgtaaat gatcgagtac atccagttcg atttcccgcg gatcgacttc
    10021 gatcgaaatc tccgcatcgg cattgaactg gaagttttcg cgcagcagct tcatcaggcg
    10081 gctgatttgc gctttattaa gatacgtcgg cgttccgccg ccccagtgca gctggctgac
    10141 gtgacgcccg gcgaacagcg gtgcacgatg gacgatttct tgctccagcg cgtccagata
    10201 ctgatcggcc ttgtgctgct ggcgagtaac aatcttattg cagccgcaga agtaacaaag
    10261 cttatggcag aacgggatat gtacgtagag agataatgga cgctcaggat agcgcgccac
    10321 ggcttgtaaa aacgcctgtt cgccgaagtc ttctgaaaac tccagcgcgg tcgggtacga
    10381 ggtgtatcgt ggcccggaat agttatattt ctggatcagg gccagatccc agtcgatttg
    10441 ctgtacagac atgctcactc cttccgatgg cgtctctggc ggctacggcg taccggcgga
    10501 tgcccgtttc gggtcatcag ccggttgcgc agccactttt gtcgccgcga caaccgctgt
    10561 agtttaacga ataaccacac cagataacat ataaccggaa gggttatcag cagaacggga
    10621 aggcccacgg cggcaatccg ttagttgccc cgcagcagac gcatcatgtc ttcttgcttc
    10681 tcgtcttctt cctcttcttc gtcatcgtca taagagaggc cgagtttctg catcaactca
    10741 tcaatacgat ccagtttagc atccacccag gattgctctt cggcactcag ggtttcgcct
    10801 gcttccagac gttccagcag cgcatccaga cgctcatccg tttctagtaa ttccaactcc
    10861 gcctgcggtg aaagcatagg tttctcactc ttcggtttgt gctgtttggt gactttttca
    10921 gtcacgccca atggaatagg ggttttactg ccaatacgtg gatctttcgg tgcgttctgg
    10981 cctttgctgc ctgacgtgtt gtttccgccc gctgcgcggc tgcccggcgc atgaccacga
    11041 cgttttttct gacgcttgcg gtcacgagct tcctgattca gctcttcgcg cgttttgcga
    11101 cgcgcttttg catgaccttt gctgcgtgat ggtgaagatg gtttcataat ggttcgtctt
    11161 ttagccgttt tattcagtat agatttgcgg cggaatctaa cagaaagcaa gcaaagaaaa
    11221 aaggcgacag attactctgt cgcctttttt cctgactcat aacccttaac gggtcttaca
    11281 atccttgcag gccgccaaca caccctgttt ttccattagc cggaacaatc cgtgttgatt
    11341 ccatttccgt ttaattacgt ctccggacgt ttgtcttcct gacaatcctc tgtcttcgcc
    11401 tcctggcgct cctgaccctc atcctgagtc attgttcctg ttagcgtcct cgctctatgg
    11461 gtgctacttt acgcctttgc tttaaacaaa caatcaaccg ccatcagtat ttgcacattt
    11521 tcagagtttt atataaaagc aattaactga tttatatagc ttatttgaca tgcatcgcag
    11581 aattttctct gctatttcgc ataaagacta tcggcaatgt ctcacaaagc gtatggcttt
    11641 tacttacaac gctcacgccg aaaaatccgc cttttattat ggttcaggta taatcgccgc
    11701 aaagctttga ctgacggaga cgaccgcttt gactaatttg aattatcaac agacgcattt
    11761 tgtgatgagt gcgcctgata ttcgccacct accttccgat accggaattg aagtggcttt
    11821 tgcaggccgt tccaacgcag gtaaatccag cgcgctgaac acgctgacta accagaaaag
    11881 cctggctcgt acctcaaaaa cccctggacg cacccagctt atcaacctgt ttgaagtggc
    11941 tgacggcaag cgtctggttg acttgcctgg gtacggttat gcggaagtcc cggaagagat
    12001 gaagcgcaaa tggcagcgcg cgctcggcga gtacctcgaa aaacgtcaga gcctacaagg
    12061 tctggtagtg ctaatggata ttcgccatcc gctgaaagat ttggatcagc agatgattga
    12121 gtgggcggta gacagcaata tcgccgtgct ggtactgctg accaaagcgg acaaactggc
    12181 aagcggcgca cgtaaagcgc aattgaatat ggtgcgtgaa gcggtgctgg cgtttaacgg
    12241 tgatgtgcag gttgaaacgt tttcttcgct gaagaaacaa ggcgtggaca agctgcggca
    12301 gaaactggat acctggttta acgagatgca gcctgtagaa gaaacgcagg acggcgaata
    12361 attttcttac cttaatgctt atgccggatg tggcgtatcc ggcccataaa ttcaatagat
    12421 tacaattcta ccgttggcct gataaacatg gtgcatcagg cattacggat ctttcctttc
    12481 gcccaataaa aaacgcccca gtcattactg actggggcgg ctaaaatatt cagccaaatc
    12541 cgattacgtg aagtaaaagg tctgaaagat agaacatctt acctctgtac cctacgcgac
    12601 taactttact cttttttgtt cagttcagaa agcacttttt gtaatttttt ttcagttgtt
    12661 gcataggaat ttctaatagc catcacaaaa cgtgacagct tatgttgctt acttacgaaa
    12721 aaagggcatg ttcaggcgaa tcttagtgcg cctgatccca gttttcgcca ctccccactt
    12781 ccaccagcaa cggcacatcc agacgggtac agttttccat cagttgatga atctgcttcg
    12841 caacggcatc gacatcatct ctatgaactt caaataccag ttcatcgtgt acctgcatga
    12901 tcatacgtac acgcggttgc tcagcctgta accacgcatc aacggcaatc atcgcccgtt
    12961 tgataatgtc ggcggcggtt ccctgcattg gcgcgttaat ggctgcacgt tcagccgctg
    13021 cacgacgagc accattgctg gatttgatat ccggcagata cagacggcgt ccgtccagcg
    13081 tttcaacgta gccctgctct ttcgcctgag cacgggtgcg ttccatatac tccagcacgc
    13141 cagggtagcg ttcgaagtaa aggtccatgt acttctgcgc ttctttacgt ggaatgttca
    13201 attgccgcgc cagaccgaaa gcactcatgc cataaatcag accaaagttg atcgctttcg
    13261 cgctacggcg ttgctcgctg gtgacggttt ccagtggcaa accaaacact tctgccgccg
    13321 ttgcccggtg gatatctttt ccttccgcga atgcggtcag caagcctttg tcacgcgaga
    13381 gatgcgccat aatgcgcagt tcaatctgcg agtagtccgc tgagacaatc acataatcct
    13441 ctggcgcaat aaacgcctgg cggatacgac gaccttcttc gttacgcacc ggaatgtttt
    13501 gcaggttagg atcggttgac gataaacgtc ccgttgcagt tacagcctgg tgataagagg
    13561 tatgcacacg cccggttttc gggttgatca tcagcggcag cttgtcggtg taagtcgatt
    13621 tcagcttcgc cagaccacga tactccagaa tcacttttgg caacggatag tccagcgcca
    13681 gttcttccag tacctcttcc gacgttgacg gcgcgccacc cggcgttttc ttcagcggtt
    13741 taatgccctg tttttcaaac agaatggttt gtaactgctt ggtggaagaa aggttaaatt
    13801 cctcacctgc aatttcatgc gctttctttt ccagctcagc cagacgaagg gtgagctctt
    13861 cagaatgatt gtgcagcact ttcggatcga tcttcacacc gttacgttca atgcgtgaaa
    13921 gtactggcac cagcggcatt tcgatattct cgaagacgtt caacggccct ttgtgttttt
    13981 gcagatccgg ccacattttc agatgcaact gcaaggtgac atcagcatct tcggcggcgt
    14041 aacgtccggc ctcttcgagg gcaatctggt taaaagtcag ttgatttttg cctttacccg
    14101 caatctcttc aaaagtgatg gttttgtgct tcaaccaacg ttccgcgagg ctgtccatat
    14161 cgtgacgccc ggcaacgcta ttgagaatgt aggactccag catggtatca aacgcaatcc
    14221 cacgcagctc aatgccgtaa ttcgccagaa taccgcgatc gtatttcagg ttttgcccga
    14281 ccttcagcgc cttttcatct tccagcagcg gttttagcaa ctcgagtgca cgctcgcgag
    14341 agatttgatc gggcgcatca agataatcat gagcaaccgg aatatatgcc gctacgcctg
    14401 gctcgatagc aaaagaaagc ccgaccaggt tagctgagat gttatcaagg ctgtcggttt
    14461 cggtatcaaa tgcaaatacc ggcgcttttt ccagcttcgc aatccacgct ttcagtgttt
    14521 cttcatcaag gatggtgacg tagttgtcat aagaaatcac cgttgccgtc acttctggtg
    14581 cttcgtctgc aacactggtt tcctgtggct tcgcggctgg ttttgcccct ttggcctgta
    14641 accatttgcc cgcttcgaca tcagcagtcc agcgtttgaa ctcatacttt ttgaacagcc
    14701 ccaacaactc ttccgctgcc ggttgctgca cttccagttg ttcacaggtc agctccagtt
    14761 caacgtcggt tttaatcgtc gccagctggt atgagagata agcaacttct ttgttttgct
    14821 cgagcttcgc tgccattgtt ttcgcgccac ggaagctcaa cccagcaatt ttttctggct
    14881 cggcatacag cgtatccagc ccgccaagac cttgcagcaa tgcctgcgcg gttttttcac
    14941 cgacgcccgg tacgccagga atgttatcag aggagtcacc catcagcgcc aggaaatcga
    15001 tgatcagttc tggcggcacg ccgtacttat tcaccacctc ttccggtccg aggatggtat
    15061 tcgtcatggt gttgataagc gtaatatttg gcgtcaccag ctgcgccata tctttatcgc
    15121 cagtgctgat cagcaccgga cgcccggctt tttcggcttc gcgcgccaga gtaccgataa
    15181 cgtcgtccgc ttctacgcca gaaaccgcca gcagcggcag tcccatcgct ttaaccatcg
    15241 cgtgcaaggg ttcgatttgt gctcgcagat cgtccggcat tggcgggcga tgtgatttgt
    15301 aatgttcaaa cagttcatca cgaaaggttt ttcccttggc gtcaaagacc accgctgcat
    15361 gcgtcggttt atattgcatg atcagactgc gcagcatgtt gaggacacca tacatcgcac
    15421 cggtcggctc gcctgcgctg ttagtcagcg ggggaaacgc gtgatatgcg cgataaagat
    15481 aagatgaacc atctacaagg ataagtggat tttgggggat ctgaaccata atgtccgtgc
    15541 ctgtttatca gattatgggt aaaggatgcc acagacagga taaaaacatg agtttttcac
    15601 cgcttttcgt gaaaatcttt tgtagatctt ctggatcgct cgcaaatcgt catgtggata
    15661 actttgtgca taaattattt acatcgcttt taatttatta tcatgctttt tacagcatga
    15721 tgtttgtctt tgtttttcat gcagttaaaa ataaatccat ccctaataat tgagataatg
    15781 ggattttttc taatgtggat agatatgaat tatttttctc cttaaggatc atccgttatt
    15841 tgggtcgttt tctgctaaaa tctgcgcctt gcgcttcttt agcgcactgt ctatggctaa
    15901 ccttttgaat aaattcatta tgacgagaat actcgctgcg ataacccttt tgctgagtat
    15961 cgtattgacc attttggtca ctattttttg ttccgtcccg atcatcattg ccgggattgt
    16021 aaaactattg ctacctgtgc cagtcatttg gcgaaaggtt tcgcgttttt gtgattttat
    16081 gatgtattgc tggtgtgaag gtctggcggt attactgcat ctgaacccac acctgcaatg
    16141 ggaagttcac gggctggaag ggttaagtaa gaagaactgg tatctgctta tttgtaacca
    16201 ccgtagttgg gcagatattg tcgtactgtg cgtgctgttt cgtaagcaca ttccgatgaa
    16261 taaatatttc ctcaagcaac agctggcctg ggtgccattt cttggcctgg cgtgctgggc
    16321 gctggatatg ccgtttatga agcgttattc ccgcgcttat ttattacgtc atccggagcg
    16381 acgtggtaaa gatgttgaaa ccactcggcg ttcttgtgaa aagtttcgcc tgcatcccac
    16441 caccattgtc aatttcgttg aaggctcccg tttcacgcaa gaaaaacatc agcaaaccca
    16501 ctccactttc caaaacttgt tgccaccaaa ggcggcaggc attgcgatgg cgctaaatgt
    16561 actgggtaaa caattcgata aactgcttaa tgttacgttg tgttatccgg acaataaccg
    16621 tcagccgttc ttcgatatgt taagcggtaa actgacgcgg attgtcgtac atgtggattt
    16681 acagcctatt gccgatgagt tacatggcga ctacatcaat gacaaaagct ttaagcgcca
    16741 ttttcagcaa tggctgaact cattgtggca ggaaaaagac cgacttctga cgtcgttgat
    16801 gtcatcacag cggcaggaca agtaaatttt gctcgcctct gaatgagcag aggcgagtga
    16861 gtattttaat gaacaaaacg tccggcacga gacataaatt cttcttcgct catctcctgt
    16921 ccgttaaaaa caactttacc cggtgtataa cgcagctgca gcgaggcggt attgttgtca
    16981 atcgtgatta actgaaacat ctgacctaat gtcatcatcc cgctgatttg tttatcagcc
    17041 tgcttttgcg ctttttccgc atccattcct tcagataaat taagctgttt tgcggtttct
    17101 gtgacgacat taagcggtaa cttcacatca aaattgagcg atttgatatc tttgtttgtg
    17161 gatgatgaag atttggctgg gtcggcaata ctgatatcca gattggcatt tagttcgccg
    17221 agtgcgttct tccatcttac cggttgttta atggtcggct cactttgttg taataacggt
    17281 aaatactctt tgaacaatgc agcattcact tcttgcagag caacttcatc gtttgctaat
    17341 tcagggtgtg cagcaagctg cttctgcatc gcaatgttat actggatgat aaattggcgc
    17401 accgctgagg gatcaataga ttcagcaatc aatgaccact caccactacc gagttgctga
    17461 ttcccttttg ttaacttatc aagttcgtag gtcagattga cattgatata atctttgtct
    17521 ttaacgcgat ccagcctggt ttttgcaacg aaagcatcga tttgggcaac gtcttccccg
    17581 tgattgataa tgttaatctg attaaatttc gactcgcttt caccaaccgg aaatttcttt
    17641 tcttctaatc gcgccagaga atcaatggta aaactttttg ccgttacttt gttcattggt
    17701 gataattgca gaacaatatc ttcgactttg ccttcaatat ccaggttaga caatgaacta
    17761 ctatcaccat taaaagtaaa ctggccctca ccaaaagaaa actttccata ctctgcaggg
    17821 gcaacatcta atgtcgtaga gaatgagcca ccaaacgcga atcgcaatgt cgcttccacg
    17881 ggcgatttat ttttcgcggc gataaatagc ggttgtgtca gttcattatt cactaagttc
    17941 acttttgccg ctgccagtgc tgggatgaca ttaccatgca ttaacattgt gatgggcaac
    18001 ggaccgtgct caacatccac atcaaataca actttctggc ctggcttgat attgagatcg
    18061 ggtgcaccgt tatcgaatgt catttgcatt tgaaaacggg aattaaaaaa gcctttgtca
    18121 aaattttgat aacttaacgt catatcgtag gcatgttcac ctttcttttt agcatcgtta
    18181 aaatctttaa taaatttttc gacaccaggc tgaatttgcg tacctgtgta ccatgtgcca
    18241 ccaccccaga ttacggctaa cgcaacaata acacctgtag ctgacttacg tatcatataa
    18301 atattccctg ataaaacagc aaaactttga atatccactt atgctgaaat aatgtccagc
    18361 ggcaggatg
//
LOCUS       DAZFAB010000092        17906 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZFAB010000092 DAZFAB010000000
VERSION     DAZFAB010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17906
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.092"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            100..1239
                     /gene="arnB"
                     /locus_tag="ACHJWA_003557"
     CDS             100..1239
                     /gene="arnB"
                     /locus_tag="ACHJWA_003557"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFO4310080.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNHALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLQGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADINAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENIARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPSLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1243..2211
                     /gene="arnC"
                     /locus_tag="ACHJWA_003558"
     CDS             1243..2211
                     /gene="arnC"
                     /locus_tag="ACHJWA_003558"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFO4310081.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHILVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREYGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2211..4193
                     /gene="arnA"
                     /locus_tag="ACHJWA_003559"
     CDS             2211..4193
                     /gene="arnA"
                     /locus_tag="ACHJWA_003559"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFO4310082.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHHCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4190..5080
                     /gene="arnD"
                     /locus_tag="ACHJWA_003560"
     CDS             4190..5080
                     /gene="arnD"
                     /locus_tag="ACHJWA_003560"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFO4310083.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKYHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQQVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            5080..6732
                     /gene="arnT"
                     /locus_tag="ACHJWA_003561"
     CDS             5080..6732
                     /gene="arnT"
                     /locus_tag="ACHJWA_003561"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFO4310084.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLTIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            6729..7064
                     /gene="arnE"
                     /locus_tag="ACHJWA_003562"
     CDS             6729..7064
                     /gene="arnE"
                     /locus_tag="ACHJWA_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFO4310085.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAISKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            7064..7450
                     /gene="arnF"
                     /locus_tag="ACHJWA_003563"
     CDS             7064..7450
                     /gene="arnF"
                     /locus_tag="ACHJWA_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFO4310086.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(7444..7710)
                     /gene="pmrD"
                     /locus_tag="ACHJWA_003564"
     CDS             complement(7444..7710)
                     /gene="pmrD"
                     /locus_tag="ACHJWA_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFO4310087.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASCYSPDEWERQCKVAGKTQ"
     gene            complement(7820..9175)
                     /gene="menE"
                     /locus_tag="ACHJWA_003565"
     CDS             complement(7820..9175)
                     /gene="menE"
                     /locus_tag="ACHJWA_003565"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFO4310088.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGF
                     AVQGVVEDSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHVATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQTEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVMEYDHESVDLSEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(9172..10134)
                     /gene="menC"
                     /locus_tag="ACHJWA_003566"
     CDS             complement(9172..10134)
                     /gene="menC"
                     /locus_tag="ACHJWA_003566"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFO4310089.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELADTLPQAA
                     NYRTAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(10134..10991)
                     /gene="menB"
                     /locus_tag="ACHJWA_003567"
     CDS             complement(10134..10991)
                     /gene="menB"
                     /locus_tag="ACHJWA_003567"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFO4310090.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(11006..11764)
                     /gene="menH"
                     /locus_tag="ACHJWA_003568"
     CDS             complement(11006..11764)
                     /gene="menH"
                     /locus_tag="ACHJWA_003568"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFO4310091.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCLEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILSFWLVGYSLGGRVAMMAACQGLAG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRSEPLTAVFADWYQQPVFASLNDDQ
                     RRELVVLRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11761..13431)
                     /gene="menD"
                     /locus_tag="ACHJWA_003569"
     CDS             complement(11761..13431)
                     /gene="menD"
                     /locus_tag="ACHJWA_003569"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFO4310092.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIACRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPKSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETVLADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13520..14815)
                     /gene="menF"
                     /locus_tag="ACHJWA_003570"
     CDS             complement(13520..14815)
                     /gene="menF"
                     /locus_tag="ACHJWA_003570"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFO4310093.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAREFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIAHHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14894..15199)
                     /gene="elaB"
                     /locus_tag="ACHJWA_003571"
     CDS             complement(14894..15199)
                     /gene="elaB"
                     /locus_tag="ACHJWA_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFO4310094.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15254..15715)
                     /locus_tag="ACHJWA_003572"
     CDS             complement(15254..15715)
                     /locus_tag="ACHJWA_003572"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO4310095.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLDT
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEIYEEDGIPHIGMAREVIQA"
     gene            15780..16697
                     /gene="rbn"
                     /locus_tag="ACHJWA_003573"
     CDS             15780..16697
                     /gene="rbn"
                     /locus_tag="ACHJWA_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFO4310096.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPSPLFQELKAGKTIMLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            16885..>17906
                     /locus_tag="ACHJWA_003574"
     CDS             16885..>17906
                     /locus_tag="ACHJWA_003574"
                     /EC_number="3.4.19.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElaD/SseL family deubiquitinase"
                     /protein_id="HFO4310097.1"
                     /translation="MMVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIELLHNIALGYDEFGKKAEDILYHIVRNPTNDTLSIIKLIKNACLKLYNLAHTATK
                     HPLKSHDSDNLLFKKLFSPSKLMAIIGEDIPLISEKQSLSKVLLNDKNNELSDGTNFW
                     DKNRQLTTDEIACYLKKIAANAKNTQVNYPTDFYLPNSNSTYLEVALNDNIKSDPSWP
                     KEVQLFPINTGGHWILVSLQKIVNEKNNTQQIKCIIFNSLRALGHEKENSLKRIINSF
                     NSFNCDPTRETPNNKNITDHLTEPEIIFLHADLQQYLSQSCGAFVCMAAQEVIEQMES
                     NSDSAP"
BASE COUNT         4295 a         4794 c         4477 g         4340 t
ORIGIN      
        1 tattctatcc ttaaaatttc gctctaaatg gcaaaatata cacaacactc tttatagcaa
       61 atataagtgg acaggtattc aatggcggaa ggaaaagcaa tgtcagaatt tttgcctttt
      121 tcgagaccag caatgggcgt ggaggaactc gctgcagtta aagaggttct cgaatccggt
      181 tggatcacaa ccggtccgaa gaatcacgcg cttgaacaag ccttttgcca gttgacggga
      241 aatcagcatg ccatcgcggt cagttcagcc accgccggaa tgcatatcac gctaatggcg
      301 ttggaaattg gcaagggcga tgaagtgatt acgccttccc tgacctgggt ttcaaccctc
      361 aatatgattt ccttgcaggg tgcaacgccg gtaatggtgg atgtcgatcg cgatacgctg
      421 atggtcacgc ctgaagcgat cgaatcagcc attacgccac gcactaaagc catcattccg
      481 gtgcattatg ccggtgcgcc agcagatatt aacgccattc gcgccattgg cgaacgttac
      541 ggcatcgcag ttatcgaaga tgctgcccat gccgtaggta cgtattacaa agggcgacat
      601 attggcgcaa aaggtaccgc tattttttca tttcatgcca ttaaaaatat tacctgtgcg
      661 gaaggaggcc tgattgttac tgataatgaa aatattgccc gccagctacg gatgctgaaa
      721 tttcacggcc tgggtgtcga tgcctatgac agacaaacct ggggccgtgc accgcaggct
      781 gaagtcttaa cgccgggcta taagtacaat ctgaccgata ttaacgccgc gattgccctg
      841 acacagttag ccaaattaga gcacctcaac acccgtcggc gcgaaattgc ccagcaatat
      901 cagcaagcac tggcagctct cccctttcag ccattaagcc ttcccgcctg gccgcacgtt
      961 cacgcctggc atctgtttat tattcgtgtc gatgaacaac gttgtggtat cagtcgcgat
     1021 gcgttgatgg aagcgttaaa agaaagaggt attggtaccg ggttacattt ccgcgccgct
     1081 cacacacaaa aatattatcg cgagcgtttt ccctcgctgt cgttaccgaa taccgaatgg
     1141 aatagcgaac gcatctgttc tttgccgctg ttcccggata tgactaccgc cgatgccgac
     1201 cgtgtcatca cagcccttca gcaactcgca ggacaataag ccatgtttga aatccaccct
     1261 gttaagaaag tctcggtggt tattcccgtt tataacgagc aggaaagctt accggaatta
     1321 atcaggcgca ccaccacagc ctgtgaatcg ttggggaaag agtatgagat cctgctgatt
     1381 gatgacggca gtagcgataa ttccgcgcat atactggtcg aagcctcaca agcggagaac
     1441 agccatattg tatctatttt gcttaaccgc aattacgggc aacattcagc gattatggcg
     1501 ggattcagtc acgttactgg cgacttaatt attacccttg atgccgatct ccagaatccg
     1561 ccagaagaaa tcccccgcct ggtggcaaaa gccgatgaag gttacgacgt ggtagggact
     1621 gtacgccaga accgccagga cagctggttt cgtaaaacag cttcgaagat gattaaccgg
     1681 cttattcagc gcaccaccgg caaagcgatg ggtgactacg gttgtatgct gcgcgcctat
     1741 cgccgtcata ttgtcgatgc gatgttgcac tgccatgaac gcagcacctt tatcccgatt
     1801 ctggcgaata tcttcgcccg ccgtgccatt gaaattccag tgcatcatgc cgagcgtgag
     1861 tatggtgaat ccaaatacag ctttatgcgc ctgattaatt tgatgtacga cctagtgacc
     1921 tgccttacca caacgccgct acgtatgctg agtctgctcg gcagcattat tgcgattgga
     1981 ggttttagca ttgcggtgct gctggtgatt ttacgcctga ccttcggacc acaatgggcg
     2041 gcagaaggcg tctttatgct atttgccgtg ctgtttactt ttattggcgc tcagtttatc
     2101 ggcatgggat tactcggtga atatatcggc aggatctaca ccgatgtccg cgcccgcccc
     2161 cgctattttg ttcagcaagt tatccgtcca tccagcaagg aaaatgaata atgaaaaccg
     2221 tcgtttttgc ctaccacgat atgggatgcc tcggtattga agccctgctg gctgccggtt
     2281 acgaaattag cgccattttt acccataccg ataatcccgg tgaaaaagcc ttttatggtt
     2341 cggtggctcg tctggcggcg gaaagaggca ttccggttta tgcgccggat aacgttaatc
     2401 atccgctgtg ggtggaacgc attgcccaac tctcaccaga ggtgattttc tctttttatt
     2461 atcgccatct tatttgcgac gagattttgc agctcgctcc cgcaggtgca tttaatctgc
     2521 acggttcgct gttaccaaaa tatcgtggtc gcgcgccgct gaactgggtg ctggtgaacg
     2581 gtgaaacaga aactggcgtt acattgcacc gaatggtaaa acgtgccgat gccggggcca
     2641 ttgtggcgca actgcgcgtt gccattgcgc cagacgatat cgctattacg ctgcatcata
     2701 aattgtgcca tgccgcgcgc cagctactgg aacagacatt acccgccatt aaacacggta
     2761 atattctgga aatcgcccag cgcgaaaacg aagccacctg ttttggtcgc agaacgccgg
     2821 atgacagttt cctcgaatgg cataagccgg catccgtact gcacaacatg gtacgtgccg
     2881 ttgccgatcc gtggccgggt gccttcagct atgttggcaa tcagaaattc accgtctggt
     2941 cgtcgcgtgt tcatcctcat gccagcaaag cacagccggg gagcgtgatt tctgttgcgc
     3001 cactgctgat tgcctgtggc gatggcgcgc tggaaatcgt caccggacaa gcgggcgacg
     3061 gcattactat gcagggctcg caattagcgc agacgctggg cctggtgcaa ggttcacgct
     3121 tgaatagcca gcctgcctgc accgcccgac gccgtacccg ggtactcatc ctcggggtga
     3181 atggctttat tggcaaccat ctgacagaac gcctgctgcg cgaagatcat tatgaagttt
     3241 acggtctgga tattggcagc gatgcgataa gccgttttct gaatcatccg cattttcact
     3301 ttgtcgaagg cgatatcagt attcattccg aatggattga gtatcacgtc aaaaaatgtg
     3361 atgtcgtctt gccgctggtg gcgatagcca cgccgattga atatacccgc aacccgctgc
     3421 gcgtatttga actcgatttt gaagagaatc tgcgcattat ccgctactgc gtgaagtacc
     3481 gtaagcgaat catcttcccg tcaacttcag aagtttatgg gatgtgtagc gataaatact
     3541 tcgatgagga ccattctaat ttaatcgtcg gcccggtgaa taaaccacgc tggatttatt
     3601 cggtatcaaa acaattactt gatcgggtga tctgggccta tggcgaaaaa gagggtttac
     3661 agttcaccct cttccgcccg tttaactgga tgggaccacg actggataac cttaatgcgg
     3721 cacgaatcgg cagctcccgc gcgattacgc aactcattct caatctggta gaaggttcac
     3781 cgattaagct gattgatggc ggaaaacaaa aacgctgctt tactgatatt cgcgatggta
     3841 tcgaggcgtt ataccgcatt atcgaaaatg cgggaaatcg ctgcgacggc gaaattatca
     3901 acattggcaa tcctgagaac gaagcgagca ttgaggaact gggcgagatg ctgctggcga
     3961 gcttcgaaaa acatccgctg cgccatcatt tcccaccgtt tgcgggcttt cgcgttgtcg
     4021 aaagtagcag ctactacggc aaaggatatc aggacgtaga gcatcgtaaa ccgagcatcc
     4081 gcaatgccca ccactgcctg gactgggagc cgaaaattga tatgcaggaa accatcgacg
     4141 aaacgctgga tttcttcctg cgcaccgttg atcttacgga taaaccatca tgaccaaagt
     4201 aggcttacgc attgatgtcg atacctttcg tggcacccgt gaaggcgtgc cgcgtctgct
     4261 ggaaatcttg agtaagcata atattcaggc cagcattttt ttcagcgtcg gcccggacaa
     4321 tatgggccgc catctctggc gactggtgaa gccacagttt ttgtggaaga tgctgcgctc
     4381 aaacgcggca tcgctttatg gctgggatat tttactggca ggtacggcct ggccaggtaa
     4441 agagattggt catgccaatg ccgatatcat tcgtgaagcg gctaaatatc acgaagtcgg
     4501 cctgcacgcc tgggatcacc atgcctggca agcccgtagc ggtaactggg atcggcaaac
     4561 aatgatcgac gatattgcac gcggccttcg cactctggaa gagattatcg gtcaaccggt
     4621 aacctgttct gccgctgcgg gctggcgtgc cgaccagcag gtgatcgaag caaaagaagc
     4681 gttccatttg cgctacaaca gcgattgtcg tggggccatg ccgttccgtc cactgctcga
     4741 atcaggaaac cctggcactg cgcaaattcc ggtgacctta cccacctggg atgaagtgat
     4801 tggtcgggat gtgaaagcag aagattttaa cggttggtta ctcaaccgca tcctgcgaga
     4861 taaaggcacg ccggtttata ccattcatgc agaagttgaa ggctgcgctt atcagcataa
     4921 ttttgtggat ctcctcaaac gcgcagctca ggaaggcgtg acattttgcc ctttaagcga
     4981 actgttatca gagacgttgc cgctcggaca agttgttcgc ggaaatattg ccggacgtga
     5041 aggctggctg ggttgccaac aaattgcggg tagtcgctga tgaaatcggt acgttacctt
     5101 atcggcctct tcgcgtttat tgcctgctat tacctgttac cgatcagcac gcgtctgcta
     5161 tggcaacccg atgaaacgcg ttatgcggaa atcagtcgag aaatgctggc atccggcgac
     5221 tggattgtgc cccatctgtt agggctacgt tattttgaaa aacccattgc cggatactgg
     5281 attaacagca ttgggcaatg gctatttggc gcgaataact ttggtgtgcg ggcaggcgtt
     5341 atctttgcga ccctgttaac tgccgcgctg gtgacctggt ttactctgcg cttatggcgc
     5401 gataaacgtc tggctctact cgccacagta atttatctct cattgtttat tgtctatgcc
     5461 atcggcactt atgccgtgct cgatccgttt attgccttct ggctggtggc gggaatgtgc
     5521 agcttctggc tggcaatgca ggcacagacg tggaaaggca aaagcgcagg atttttactg
     5581 ctgggaatca cctgcggcat gggggtgatg accaaaggtt ttctcgccct tgccgtgccg
     5641 gtattaagcg tgctgccatg ggtagcgacg caaaaacgct ggaaagatct ctttatttac
     5701 ggctggctgg cggtcatcag ttgcgtactg acggttctcc cctggggact gacgatagcg
     5761 cagcgggagc ctgacttctg gcactatttt ttctgggttg agcatattca acgctttgca
     5821 ctggatgatg cccaacatag agctccgttc tggtactacg tgccggtcat cattgccggt
     5881 agtctgccgt ggctgggatt actccccggt gcactataca caggctggaa aaaccgcaag
     5941 cattccgcaa ccgtctattt gttgagctgg acgataatgc cgctgctgtt tttctccgtt
     6001 gctaagggta aattgcccac ctatattctt tcctgctttg cacctttggc aatgctgatg
     6061 gcgcattacg ctttgctggc agcaaaaaat aatcctctgg cgctgcggat taatggctgg
     6121 attaacatcg cttttggcgt cactggcatt attgccacat ttgtggtctc cccctgggga
     6181 ccaatgaaca cgccggtgtg gcaaaccttc gagagctata aagtcttttg tgcctggtcg
     6241 attttttcgc tatgggcatt tttcggctgg tacaccttaa caaacgtcga aaagacctgg
     6301 tcttttgccg cgctttgccc gctggggctg gcgttgctgg taggattttc aattcctgac
     6361 agagtcatgg aaggaaaaca tccgcaattt tttgtcgaga tgacacaaga atcacttcag
     6421 ccaagccgct atattcttac tgatagcgtc ggtgttgccg caggtctggc atggagtctg
     6481 caacgcgatg acatcatcat gtatcgccag acaggtgagt tgaaatacgg ccttaattat
     6541 ccggatgcga aagggagatt tgtcagcggt gatgagttcg caaactggct taatcaacat
     6601 cgtcaggagg ggattattac actcgtgctt tcggttgacc gcgatgaaga tatcaacagt
     6661 ctcgccattc cgcccgcaga tgccatcgat cgtcaggagc gtctggtgct gattcagtat
     6721 cgtcccaaat gatctggcta acattagtct ttgccagctt gcttagcgtt gccgggcagt
     6781 tgtgtcagaa acaggcaacc tgctttgtgg cgataagcaa acggcgcaaa catatcgtgc
     6841 tgtggctggg actggcgctg gcttgtcttg gtcttgccat ggtgctctgg ctgctggtct
     6901 tgcagaacgt accggtaggt attgcctacc cgatgttaag tctgaatttt gtctgggtga
     6961 cgctggctgc agtaaaactg tggcacgaac cggtatcgcc gcgtcactgg tgtggggtgg
     7021 cgttcattat tggtggcatt gtgatcctcg ggagtacggt gtaatgggcc tgatatgggg
     7081 attattcagc gtcattattg cctcagttgc gcaattaagc ctgggttttg cggcgagtca
     7141 tctgccgccg atgacgcact tatgggattt tatcgcggcg ctgttagctt ttgggctgga
     7201 tgcccggata ctgttactcg ggctgctggg ttatctgctt tccgtttttt gttggtataa
     7261 aacactacat aaacttgcgc taagcaaagc ctatgccttg ctcagtatga gttatgtgct
     7321 ggtatggatt gcttcaatgg tacttcccgg ctgggaaggg actttttcgc tgaaagcact
     7381 actgggagta gcctgtatta tgagcgggtt gatgctgatt ttcctgccca cgacaaaaca
     7441 acgttactga gttttccctg ccactttaca ctgccgttcc cactcatcgg ggctataaca
     7501 gcttgcagag aggactttca ccgtatgcag cttttcacga ttaatacaat aaagcgcatt
     7561 ctgcaaaggc gagagtaaat cccccacttt cacggcgaag tcactcttca cttcggcgat
     7621 catttttatc gcgccgccag catcgcagag cataagtaca tgtctgttat cttgtttatt
     7681 gcagcacgat tttttgacca gccattccat tgtattatcc tgtttgctaa gagttttcat
     7741 atcaatttac agattaacag ataaactctt tatcaacggc aatggaatgg cagaactgag
     7801 aagtgaaacg gagggttaat tattgctgac gttgcaccca ctcttttagc gcctgacgtg
     7861 aaattttaat accgccgttt ttcagctccg gcggcaaagt cagccaacgt accggttgct
     7921 gaaaacgggc cagcttatct ttcacccatt cactaagatc aacgctctcg tggtcatact
     7981 ccatcaccgc caccggtcga tgaccaaact ccttgtcggc aacggggacg ataaacacct
     8041 gcaaaaccgc aggatgtgca gcaattacgc gctcgacttc ttccgtctga ataccctctc
     8101 cgccactgaa gaatagattg tctaaacgtc cgacaatggt cagcttgcca ttatgcatct
     8161 caccgcgatc gcgcgtagcg taccagcctt cgtcattaac cagtgaaacc agttgcccgt
     8221 tacgccagta accttctgcc atactggcag cccgcagcca cacttcatta ttaacgattt
     8281 tcacttcccg acccggcagc ggcgaaccaa cgtctgccag gccgtcggct tctttcgcac
     8341 acaccgtgga ggcaaactcg gtcagaccat agccgcaaaa gcaacgaatc ccctgctcgc
     8401 gcgcctgttc cgtcaactcg accgggatag ccgcgccgcc aagtaacacc gctttcaggg
     8461 aaacggaact gcggttaacc agcaaacgcc agagttgtgt tggcaccagt gaagcgtgag
     8521 tacagcctgc cagcatttgc tccaatggct gtttatcacg caccgtcatc cgcgcaccag
     8581 cgtataacca gcgccacata attccctgac cggagacgtg aaacagcggt aaagagagca
     8641 accagtcatc gtgatcgcca aacggaatca gcgataacac cccttcggca ctggcaagat
     8701 gggcctgata agtatggaca gcggctttcg gcaacccggt agaacccgag gtcaacgtca
     8761 ttgagcacag acgcgtcggc tgccacgtag cgacatgtgc gccttcaacc cgctgaatgt
     8821 gcagcgacgt taatgccgga aacgtgtttt ccccatccgg caccagcgca aattgcagcg
     8881 tcagattggg cagcaattct tcaagcaacg gttgcggcag ctgagggttc acgggcaaca
     8941 cccgcgcccc gcattgcagt aacgccagcc aagcgagcag cgtttgcggc gtattccacg
     9001 cccgcaacat cacgccgctg tcctcaacca caccctgcac cgcaaatccg gaggctaatt
     9061 catcgacgcg agcacaaagc tcgcgccagt tgagttgctc gtcattaaga cgtaaggcga
     9121 tggcttctcc ccgcacttgc cgccagtgac gccacggcca gtcagagaag atcataacaa
     9181 ccgctccagt gcatcaactt ccacgacagg cagcgtgcta cccggccagc gacgtacctg
     9241 ctgcgcctgc attagatcca gcgtgtccag ccctggaatg gtgtccggcg ttaaccaggc
     9301 agcaatccgc gccagttgcg ttaagcctaa gctcgattca atggaagaac tgatcactgc
     9361 cgtcagcccc agcgcgtgcg ccgcctgtac ctgctcgcga actttctcca gactgcccgt
     9421 gagcgtgggt ttgataacta ccgcgcgcac gccctcttca gccacaaagg caaaatccgg
     9481 ctcgcgcaag ctttcatccc aggcaatggc aatgccggtt tcacgggcaa acgctcgcga
     9541 atcatcgcgg gttttgcacg gctcttcgag aaacgcgatg cggtggcgat aatccgggtt
     9601 aacgtatttg gcaaactgct gacctttcag cggtgtccag gcgcgatttg cgtcaagacg
     9661 caaatgcaga tccggaattg cctccagcaa cagattcacc accatgccgt cgcgcaccgc
     9721 ttcgtacaat cccactttga ccttcgccac tttctcgcct ggcatatctg caagtttgag
     9781 gatcagatcg tccggatcgc cattacacag tggtgccgta cggtaattgg ccgcctgcgg
     9841 gagcgtatct gccagctccg ccaatgcaca acttacgcca aaggcaacgg aaggcatctg
     9901 cggtaattcg caatcgcctg ccagccagtt atttacccag gcaagcagca cactttgcgc
     9961 ctcttcccag gtttcctgac tgaagcccgg cagtggggag atctcccccc acccttcgcg
    10021 ctcgccctca cgcaggcaaa cgtacagccc gtcgcgggtt tttaaccgcc tgtcgcgcag
    10081 aaccaccccc gcgtccatgg ggatctgcca gcggtatacc tgcgcgctac gcattacgga
    10141 ttccgtttga atttgctgaa gtcaggctga cgtttctggt tgaaggcgtt gcgaccttcc
    10201 tgaccttctt ccgtcatgta gaacagcatg gtggcgttgc ccgccagctc ctgcagacct
    10261 gcctgaccgt cacagtcggc gttcagtgca gctttcaggc agcgcagcgc catcggactg
    10321 ttttgcagca tttcacggca ccagcgaacg gtttcttttt ccagatccgc cagcggtacc
    10381 acggtgttga caaggcccat atccagcgcc tgttttgcgt cgtactgacg gcacaggaac
    10441 cagatttcac gcgctttttt ctgcccgacg atacgagcca tgtaggaagc gccccagccg
    10501 ccgtcgaagg aaccgacttt cgggccagtc tgaccgaaga tggcattatc tgccgcgata
    10561 gtcaggtcgc acatcatgtg cagaacgtga ccaccgccga tggaatagcc agccaccatc
    10621 gcgacaaccg gtttcggaca ggtacggatc tgacgctgga agtccagcac gttcaggtga
    10681 tgtacgccgg aatcatcttt atagccgccg taatcaccac gcactttctg gtcaccaccg
    10741 gagcagaacg ctttatcgcc tgcgccagtc agaatgatca cgccgacgtt atcgtcataa
    10801 cgcgcatctg ccagtgcctg gatcatctct tttaccgtca gaggacggaa ggcattgcgc
    10861 acctgcggac gattaatggt gatttttgcg ataccgtcgg tggatttttc ataacgaatg
    10921 tcctcaaagc cttcggagca gtcgtgccat tcaaccggtg cgtaaagcat tgcttcatca
    10981 ggataaatca tattgtgtcc tttagtcaga aacgcaagat ctgcgccaga cttgcgatta
    11041 cgccagcggg attttcccga tgcgcgttat gtccggcgcg aggaatgaca tggcagtcgg
    11101 cagccagttc cgccgccagg gcgcggaatt tgctgtcacg ttcaccacat aaataataaa
    11161 acgcaaatgt gcgggcgcta aggttagcac gtaaatcagg ctggacggcg agagaagtcg
    11221 cctccagcat ggcggcaagg gttgcgccat tattgttgct gcgcagcacc accagctccc
    11281 ggcgttgatc gtcattgagc gaggcaaaaa caggctgttg ataccagtcg gcaaataccg
    11341 cggttaatgg ttcggaacga aaacgctgcg cccattggcg atcggaacgc tgacgttccg
    11401 cgcgttgttc agcattttgc agccccggat gaccgccttc gacaacaacc ccacaaagcc
    11461 ccgccagccc ctggcaagcg gccatcatcg ccacccgtcc gccaagcgag taccccacca
    11521 gccagaagct aaggatgttg taactaacca gggttttacg cagtaagtcg gtgacatcat
    11581 caaatccatc gacgctaatc gccgccgaac caccgtgacc tgggagatca acatacaacc
    11641 gtgagtagtc ggcaaacgcc tcgccgactt cctgccattc gaggcaatcg ccggaaaaac
    11701 cgtgcaaaaa caccagccag ggtaaacctg gtttgccgtg ttttgcctgc gcgtgcagga
    11761 tcataaatgg cttacctgcg ccagcagctg ctggagcgtt tgcgcgccgt cggtgtcgtt
    11821 aaccaccatt tcaattaccg tggtggtcgg agtacgccag gcgtcggcaa gtaccgtttc
    11881 aagttcctgc cagttttgcg gacgatgata tttcagctca aacatcgcgg cagcgtgctc
    11941 aaaatggacg ttttgcggca tcagatagaa acgctcgcgc tcgcttttcg gcgttggcag
    12001 cagcgagaaa atttgcccgc cgttgttgtt caccacaatt aataccagcg gcgcggaaac
    12061 ctggcgcaat aacgccagcg cattcaggtc gtaaagtgcg gagagatcgc ccacaatcgc
    12121 cagcgtcggt ttgccgcttg cccgctgaac gccggcggca gtcgagagca gcccgtcgat
    12181 accgctggcc ccacggttgc tgtacaccgg gtaacctgcc ggaagttgcg aaagcgcatc
    12241 aatcagacgt accaccaggc tgttaccgac aaacaattgc ccctgttcag gcagatagtc
    12301 gctgatgcga tgcgccagtt gcgcttcgcc aaacgcatcg cgacaggcaa taaccgcctg
    12361 cattgcctgt tccgccaggc gcgggatttc aacgcaccag ggctggcgtt tttctgccgg
    12421 atgcagttcc agccagtcgg caatattggc aattaagcgg cgtccacgat ggtgtgccgg
    12481 atcaagccgc ccttcgatgt catccacaat ccagtactct tcgggttcac agcttgcctg
    12541 ccattgcagg agccgtttgc ccgtcaggct gcttcccagt tgcaccacaa tttgcgcctg
    12601 ttgcagttcg ctggtcgctt tggcattgcc taaccagaga tcggcacacg gcagcggctg
    12661 tccggtttgc gacagcacgt cgccaatcag cggccagcca agagtttgcg cccacagggc
    12721 aacttttttg ccctcttccg cactcatgcg cccggcaacc accacgccgc gcttttgccg
    12781 ccagaagaac cagtcgcgct gtttttcgct ctcaagacga ggcgcttcac gcagccacgg
    12841 cttatcatcc tgccaccagt cacccagacg ctgttgccag ctaagcccag tgtcgtccat
    12901 ctcgccatac agcggttcag caaacgggca gttgatatgg accccaccag catgaagcgt
    12961 accgagagca tggtcgatgg tagaaaccag ccaacgtgcg gggatatcct gggtcgggcg
    13021 cggcagtgaa atactgtgcg tggggtgaga ggcgaacatt cccggctgac gaatcgcctg
    13081 attcgcgccg cagtcaatta gctccggcgg gcgatcggcg gttaacagga tcagtttttc
    13141 tccggttaac ccagcttcaa tcagtgccgg atagagattt gctaccgccg tgccggaggt
    13201 cacaatcacc gccaccggct gcttgctgac tttcgccagc cccagcgcca gatgccccag
    13261 tccacgctca tcgaaatggg tgtggtgaat gaatgcggaa ttctccgccg ccgctaacgt
    13321 taacggtgta gaacgcgagc caggggcgat gcagatgtgt cttacgccgt gacgcgttaa
    13381 tgcttccaga atgaccgccg cccagcgtcg gttaaatgcg cttactgaca tgagtttgtc
    13441 cggtatcaat attgcggcta agtataagga gctacaaaaa tagattattg atatgaatcg
    13501 gtaatgatgc gactcattac tattccattt gtaataaagt acgcagccct gccgctttgt
    13561 tgtcgatttc ctgccactct tgctcggggt cggaaccacg gacaatgccc gcgccagcat
    13621 atagccgcac gacattgccg ctgatttttg ctgagcgcag ggaaacgcag aattcgcttt
    13681 gttgtaatga gagatagccc gcagaaccgg cgtaccattc tcgggtgaac ggttcgtgat
    13741 gggcgataaa ctgtcgcgcc agatcgcgcg gtaagccagc aactgctgcc gttggctgca
    13801 actgatgtaa acagatcaca tcatccgctt tgttgagtga agtccagata cagcggcgaa
    13861 gatgctgcac tttacgcaga cgcagtacct gcggcggtaa aacatccagc gtctgggtat
    13921 cggcctgtaa tcgttggcag atatcttcca ccaccagcat gttctcgcgc tggtttttat
    13981 catccgccat cagccactct cctaactgct gcgcctgctt atcatcagga tgatttgcta
    14041 ctgttcccgc cagcgcttca gtacgcagcg ctttgtcacg ccgccgccat aaccgttccg
    14101 gtgaagagcc aagaaaagca ttttcgccat caaaggccat gtaaaaatgg tagcaattga
    14161 gattcagtcg acgactggca gccatcatcg ccgccgcatt gaccggactt gcgaaatgca
    14221 ggtcagttgc ccgagcaagc accactttgt cgagctcacc ttcggcgatg gttttcgttg
    14281 ccagttcgat taattgcgtc cagcccgttt tgtccggcca gtgttgttct cgcgtagtgg
    14341 ttaaatgtaa cccaggcaag ggcttgatac tcaccagtgt ggcgataaat tctcttgcct
    14401 taatcgcatc gtgctgaagg gagctttcgc tgaatagcgt cagccgcagc gtggctttac
    14461 cgccacagcg tcgccattcc aggcgtggta aaagtaaatt gccctgcgac gggtcaaacg
    14521 cattcagccc ccaaatgcgt aagtcggcgt gttccgggtg ctggcgaaga aaacgttgtg
    14581 cctggtccaa cgacgtaaaa cgggtaatcg cgcccaggac ggcagcttct tcatcaccat
    14641 tacgttgttg ccagtagaat tgcgggtacg tttgctgact ggccagccag ctcaaggcat
    14701 caaaagcgtc tttgagaggg aaaggaatat cgataacccg aatgccgggt gtcgccggaa
    14761 tctcttgcga caaatggcgc agtagatttt ccagcgccgt agtaagtgat tgcacgcgga
    14821 cctctccctg ttaaaagcct cacattatac ggggtactac aaaaaaaatg cagtaccccg
    14881 gtgtagggag gttttaacgg cgtgccagca acagtcctag taccagccca acggccgcac
    14941 ccacaccaat tccctgccag ggtttttcgt ggacgtagtc atcagcacga taaacagcct
    15001 gcttcgcccg atagtaataa ctgtctgaag cctggctaac ccgttttttc acatcatcca
    15061 gcgctttttc tgcacgcgct ttcagctcaa catatttctg atcggcggga tcgccagagg
    15121 agcggagcac ctcttccagt gtttcactaa gcagcgtcag gtcgtcatcg atacgtgtat
    15181 caccaaactg attagacatt ctcgttctcc atttgcgtaa aacctgtcac taactataga
    15241 caagggttat tgattacgcc tgaattacct cgcgtgccat cccaatgtgt gggataccat
    15301 cttcttcata gatctccgtc acagggataa aaccgaaact ctggtagaag ttttgcagat
    15361 gcgcctgcgc cccgaggtag acaggcttat caggccagtg atgcgtgcaa gtgtcgaggg
    15421 ttttactcat cagttgctgc cccacttttt cgccacgcaa cgcttcactg acaatcaccc
    15481 gaccaataac gaccggctca agatcatcat cacttttcag aatcctcgca tatgccacca
    15541 gttcatcatt tttccaaccg agaatatggc gattatcccc cgtcaaatca tcaccatcga
    15601 tatcctgata agggcaattt tgctcgacaa caaacaccgc gcaacgcagt tgtaataagg
    15661 cataaagctg ggaaaccgat aattcagagt gatgcagatc ttgccattca atcataatgc
    15721 tctcctttta gatgtatgcc ttatactagc cggaatattt tttgaaacgt gatgaactca
    15781 tggaattaat ttttttaggt acttcggccg gtgtgccaac ccgcacgcgc aatgtcacgg
    15841 caatattgct aaacctgcaa catccgaccc agagcggact ttggttgttt gactgcggtg
    15901 aaggcaccca gcatcagcta ctgcataccg cctttaaccc tggaaaactg gacaagattt
    15961 ttatcagtca ccttcatggc gatcatcttt ttggtttacc cggcttgctg tgcagtcgtt
    16021 ctatgtcagg cattatccaa cccttaacga tttatggtcc gcaaggtatc cgtgaatttg
    16081 tggagaccgc gctgcggatt agcggctcat ggaccgatta tccgctggaa attgtcgaaa
    16141 ttggcgctgg cgaaattctc gatgatggcc tgcgcaaagt gaccgcttat ccaatggaac
    16201 acccactgga atgctatggc tatcgtattg aagaacatga taaaccgggt gcattaaatg
    16261 cccaggcatt aaaagctgct ggcgtaccgc ctagcccact gtttcaggaa ttaaaagcgg
    16321 gcaaaacaat catgctggaa gatggaaggc agattaacgg cgcagattac ttagctgctc
    16381 cagtgccagg taaagcgctc gctattttcg gcgataccgg cccctgcgat gccgcacttg
    16441 acctggctaa aggtgtcgat gtcatggtgc acgaagcgac gctggatata accatggaag
    16501 ccaaagccaa tagtcgcggt catagctcta cacgccaggc tgcgacactg gcccgtgagg
    16561 ctggagtcgg taagctaatc attacccacg tcagctcgcg ctatgatgac aaaggttgtc
    16621 agcacctgtt acgtgaatgc aggtcaattt tcccggcgac tgaactagcg aatgatttca
    16681 ccgtgtttaa cgtttaactc tgcttattat taaaaacagg gcgaaacttg ccctgttatc
    16741 gcaacccgcg ctacatatat ccactcacac gggcatattg agcgattttt gatatttatt
    16801 catctcctta cgcactatat tcagagcaat aagttgcctc tttttatctt cattcagcac
    16861 aacctttgca actaaaagga gcatatgatg gttacagttg tcagcaatta ttgtcaatta
    16921 tctcaaaccc aactcagtca gacatttgca gaaaaattta ctgtgaccga ggaattactg
    16981 cagtctttaa aaaaaacagc gttatccgga gatgaagaaa gcattgagtt actgcataat
    17041 attgcgttag gttatgatga atttgggaaa aaagctgaag atattcttta ccatattgtt
    17101 agaaacccaa caaatgatac cctatcgatt atcaagctta taaaaaatgc ctgtttaaaa
    17161 ttatataatt tggcacatac cgcaaccaaa caccccctca aatcacatga ttcagataat
    17221 ctcctgttca aaaaactatt ctccccttcg aaattaatgg caattatcgg tgaggacatt
    17281 cctctcatat cagaaaaaca gtcactttca aaggtacttt taaatgataa gaataatgaa
    17341 ctgagcgatg ggacaaactt ctgggataaa aatcgtcaat taaccacaga tgaaatagct
    17401 tgctatctta aaaagatcgc cgccaatgca aaaaatactc aagtcaatta tcctactgat
    17461 ttctacctcc ccaattccaa cagcacttac ctggaagtcg ctctcaatga taatattaag
    17521 agcgatccat catggccgaa agaagtccag ttattcccca taaatactgg cggacactgg
    17581 atattagttt cgctacagaa aatagttaat gaaaaaaaca acacacaaca aataaaatgc
    17641 atcatattca attcattacg tgcactaggc catgaaaaag aaaattcact taagcgtatc
    17701 attaacagtt tcaatagttt caattgtgac cccacgagag aaacgccgaa taataagaat
    17761 ataacagatc atttaactga accagagata atatttttac atgccgatct tcagcaatac
    17821 ttaagccaaa gttgcggtgc atttgtgtgc atggcagccc aggaagtgat tgaacaaatg
    17881 gaaagcaatt ctgacagcgc ccccta
//
LOCUS       DAZFAB010000093        17905 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZFAB010000093 DAZFAB010000000
VERSION     DAZFAB010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.093"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            220..945
                     /locus_tag="ACHJWA_003575"
     CDS             220..945
                     /locus_tag="ACHJWA_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4310098.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            903..1580
                     /gene="bglJ"
                     /locus_tag="ACHJWA_003576"
     CDS             903..1580
                     /gene="bglJ"
                     /locus_tag="ACHJWA_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFO4310099.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCET
                     SNVLHPY"
     gene            complement(1618..2406)
                     /gene="fhuF"
                     /locus_tag="ACHJWA_003577"
     CDS             complement(1618..2406)
                     /gene="fhuF"
                     /locus_tag="ACHJWA_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFO4310100.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDARLAQAVRATIAEHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIREYKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTTGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2547..2783
                     /locus_tag="ACHJWA_003578"
     CDS             2547..2783
                     /locus_tag="ACHJWA_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFO4310101.1"
                     /translation="MLQRTLGSGWGVLLPGFLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
     gene            complement(2822..2908)
                     /locus_tag="ACHJWA_003579"
     tRNA            complement(2822..2908)
                     /locus_tag="ACHJWA_003579"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2872..2874),aa:Leu,seq:cag)
     gene            complement(3098..4129)
                     /gene="rsmC"
                     /locus_tag="ACHJWA_003580"
     CDS             complement(3098..4129)
                     /gene="rsmC"
                     /locus_tag="ACHJWA_003580"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFO4310102.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFQHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            4232..4645
                     /gene="holD"
                     /locus_tag="ACHJWA_003581"
     CDS             4232..4645
                     /gene="holD"
                     /locus_tag="ACHJWA_003581"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFO4310103.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            4614..5060
                     /gene="rimI"
                     /locus_tag="ACHJWA_003582"
     CDS             4614..5060
                     /gene="rimI"
                     /locus_tag="ACHJWA_003582"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFO4310104.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            5075..5752
                     /gene="yjjG"
                     /locus_tag="ACHJWA_003583"
     CDS             5075..5752
                     /gene="yjjG"
                     /locus_tag="ACHJWA_003583"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFO4310105.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLNVEPGKLNEAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            5843..7432
                     /gene="prfC"
                     /locus_tag="ACHJWA_003584"
     CDS             5843..7432
                     /gene="prfC"
                     /locus_tag="ACHJWA_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFO4310106.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            7825..8430
                     /gene="osmY"
                     /locus_tag="ACHJWA_003585"
     CDS             7825..8430
                     /gene="osmY"
                     /locus_tag="ACHJWA_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFO4310107.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGIVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            8557..8718
                     /locus_tag="ACHJWA_003586"
     CDS             8557..8718
                     /locus_tag="ACHJWA_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFO4310108.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            8840..9913
                     /locus_tag="ACHJWA_003587"
     CDS             8840..9913
                     /locus_tag="ACHJWA_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFO4310109.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAYICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            9910..10692
                     /locus_tag="ACHJWA_003588"
     CDS             9910..10692
                     /locus_tag="ACHJWA_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO4310110.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGESGLDL
                     FGDDPQFERQQWFLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLSRTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRVVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFDVLCELRPEPEDEIAEVLLNNTYAVFNVRG"
     gene            complement(10906..11769)
                     /locus_tag="ACHJWA_003589"
     CDS             complement(10906..11769)
                     /locus_tag="ACHJWA_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFO4310111.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAAFIKGVGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALRERG
                     VSRLIFPALYL"
     gene            complement(11741..13290)
                     /locus_tag="ACHJWA_003590"
                     /pseudo
     CDS             complement(11741..13290)
                     /locus_tag="ACHJWA_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
     gene            13548..14327
                     /gene="deoC"
                     /locus_tag="ACHJWA_003591"
     CDS             13548..14327
                     /gene="deoC"
                     /locus_tag="ACHJWA_003591"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFO4310112.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            14454..15776
                     /gene="deoA"
                     /locus_tag="ACHJWA_003592"
     CDS             14454..15776
                     /gene="deoA"
                     /locus_tag="ACHJWA_003592"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFO4310113.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            15828..17051
                     /gene="deoB"
                     /locus_tag="ACHJWA_003593"
     CDS             15828..17051
                     /gene="deoB"
                     /locus_tag="ACHJWA_003593"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFO4310114.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            17108..17827
                     /gene="deoD"
                     /locus_tag="ACHJWA_003594"
     CDS             17108..17827
                     /gene="deoD"
                     /locus_tag="ACHJWA_003594"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFO4310115.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
BASE COUNT         4326 a         4548 c         4793 g         4238 t
ORIGIN      
        1 agatatttta aatgtctcca ggctatttca ccaggttatc atttgatgct aaccttctga
       61 cacatgcagt ggagttgttg tgcagcagga gtatgctgat atgaaagtga atgctaagga
      121 taatttattc gcttaatcta ttaatttgct ggaaacattt aaggaatgaa tatcctgaat
      181 aaaccatcat aatttttaga cagtggatgt ggaggaaata tgttgccagg atgctgcaaa
      241 aatggaattg ttatcagtaa aatacctgtt atgcaagcag ggttaaaaga ggtcatgagg
      301 actcacttcc ctgaatatga aataatatcc agcgcctctg cggaggacct taccttatta
      361 caattacgtc gttccggatt agtcattgct gatttagccg gtgaaagtga agatccacgt
      421 tctgtttgtg aacattatta ttctttaatc tcacaatatc gggaaattca ctgggttttc
      481 atggtgtcac gctcttggta ttcccaggct gtagaactgc tcatgtgccc tacggcgacg
      541 ttattgtctg atgttgaacc cattgagaat ctggtcaaga ccgtacgttc cggcaatacg
      601 cacgcagagc gtatcagcgc catgctgacc tctccggcaa tgactgaaac tcatgatttt
      661 agctatcgct ccgtcattct cactctttca gagcgcaagg tactgcggct attaggtaaa
      721 ggatggggca tcaaccagat agcttcattg cttaagaaaa gtaataaaac tatcagcgcc
      781 caaaaaaaca gtgcgatgcg tcgactggca attcacagca acgctgaaat gtatgcatgg
      841 ataaatagcg cgcagggtgc aagagaactt aacttgcctt ctgtttatgg agatgccgca
      901 gaatggaaca cagccgaatt aagaagagaa atgtcgcact catagaaaaa tgcgtcatga
      961 gtagtatcgg tattgagagt ttattcagaa agtttgcggg caacccttat aagctccata
     1021 cctataccag tcaggagtca tttcaggatg ccatgtcgcg gatctcgttt gcggcggtca
     1081 ttttttcttt ttctgccatg agaagtgagc gcagagaggg attatcttgc ctgactgaac
     1141 tggcgattaa gtttccgcgt acccggcgtt tagttattgc ggatgatgat attgaagctc
     1201 ggctgattgg ttcattgtcg ccatcaccgc tggacggtgt attaagtaaa gcgtcaacgc
     1261 tggagatttt tcatcaggaa ctctttttgt cattaaatgg tgtacgtcag gcgaccgacc
     1321 gactgaacaa tcagtggtac attaaccaaa gccggacgtt aagcccgacg gagagagaaa
     1381 tattgcgctt tatgtcgcgt ggctactcaa tgacacaaat tgccgagcag cttaaacgca
     1441 atatcaaaac gatccgtgca cataaattta atgtgatgtc gaaactgggc gtcagttctg
     1501 acgcagggtt gttggaggcc gcagatattc tgttatgtat gcggcattgc gaaacaagta
     1561 atgtgttgca tccctattaa tccgcatgat gccgggttta cttccccggc agtgctttca
     1621 tttcagcgta caatcgccac attgctgtac atccggtaag cgataacgct ggcagcaagt
     1681 gcggcgcacc agcaggccgt cgcgtagcac cacggtacgc cagagtggat tatcttcacc
     1741 ggtagtaagc gttttctcaa aaaagagggc atggcgcagc gattcaaccg tcgcctcgcc
     1801 gagcagttgc ttcatctcag taagatacca gttgatcaaa taaccggtat tactccagat
     1861 aagtttgccg ttgatctctc cggtcgcttc tagtgcctgc acaaccggaa ccagcgcctg
     1921 gctgattaac gtttccattc gctgctgcgg cgaatgtggt gttgcgtttt tatcttcaca
     1981 cacatcgacc cagaaacagg cgacgcgtcc ggtttcgtga aactcagcat ggaaatgttc
     2041 cggcgacaca tctaatgctt tttcctgcgt cagtagcgcc agcattaatg gtggcaccat
     2101 caggccgata taccattgtg cccatagtga gatcagcggt ttgtactcgc ggatcatcgt
     2161 tggttggttg cgatagatat gatcggaata gaccgccagt agagaactta gcgcattcgg
     2221 tgatgaccat tgcgccagcg tcatggcgtt aagcggagcg ggttcatcca ggcggataaa
     2281 ctccagcaaa tgttcacgat gttcggcgat cgtcgcacgt acggcttgtg caagcctagc
     2341 atcctgtggc tggagatgcg ttcgccagat gatatcttca tagagcggtg cggaacgata
     2401 ggccataatc gggatagtaa tctaaatgat aatgattgct aatcctagcg ataggtttac
     2461 ccgatagcaa gggatttctc tggcttgcaa gtgataaaaa ttatcatatg atattggtta
     2521 tcattatcaa tgaaagagat aaaaccatgt tgcaacgtac gctgggcagt ggctggggag
     2581 tgttgctgcc gggatttctg attgcagggc tgatgtatgc ggatttatcg ccggatcagt
     2641 ggcggattgt cattctgatg ggattagtat tgacgccggt gatgctgtat cacaaacagt
     2701 tgcggcatta cattttgcta ccatcgtgcc tggcgcttat tgctggcatc atgctgatga
     2761 taatgaattt gaatcaggga tgaaaaaaca gggaagaaac aagaaaggaa gtaaagataa
     2821 ttggtgcgag gggggggact tgaaccccca cgtccgtaag gacactaaca cctgaagcta
     2881 gcgcgtctac caattccgcc accttcgcat accatcaatt cttaaaaaga attgctacca
     2941 cggaggcgca ttctagtggt tttcagcttt tcgtcaatag ttaattatcg cctgatgtgt
     3001 aattgctgga aaaatgtcca tcaggagact aacgtgcagg ttttgtaagt cggataaggc
     3061 gttcacgccg catccgacac ggtattcggc gagataatta acctttcttc gcctggcggg
     3121 tcataatggc gcgatacacc ttgaagcgcc ctgtttgcgc gatcacttcg tggaagccaa
     3181 atgtctcatc cagcacgtcc ggataaggca ggaaggcgtt cgctacaatt cgcagctcgc
     3241 cgccgctatt aagatgacgc accgcaccgc gaatcagcgt ttgcgccgca tccaggctgg
     3301 tttgcatccc atcgtggaac ggcgggttgg agatgatcat atcaaaacga cctttcacct
     3361 cggaaaagac gttgctggca aagacttcac cttcaacacc gttggccgca agtgttgcgc
     3421 ggctggcttc taccgctggc gcagagacat cgcacaaggt gagacgaatt ttcggcgaat
     3481 ggcgcgcaaa ggcaaccgaa agcacgcccg cgccacagcc gacatccagc actttacctt
     3541 tcgtgtgcgg ggttaacgtc gagagcagca actggctacc gacatccaga ccgtcgcggc
     3601 taaacacgcc aggcagcgtt ttgaccgtca ggccatcgac gctgtattcg ccccagaatt
     3661 tatcggcatc aaataccggc tgtttttcca gacgaccaaa atagaggcca cagcgacggg
     3721 cgctgtcgac tttattcaac ggcgcataat cagccagcat ctgctcggcg ctgcgcacgc
     3781 cgctgcggtt ctcgccaaca acaaaaatat ccgtccccac cggaagcaga gaaagtaaat
     3841 tcatcaactg gaactgggct tccggtttgt tcttcggcca gtagtaaatc agcgtatcgc
     3901 aatctgcgac atcatccgcc gtggcgacca gactaaaacg ggcgttatcc cccatctggc
     3961 ggcttaacac ctgccaatgc tggaattgct gggtatgagc acggctggcc gcggtatcta
     4021 aacgcgcggg caggtcatcc tgtaagtctc cggcaaacag aatacggctt tgttcgaaat
     4081 catcactgtg acgcagcaag acttcacttg ccggggtaaa tgcagacatg gaatgctcct
     4141 caattgatac tggcggcgat tatagccata tgttggcgcg gtatcgacga atttgctata
     4201 tttgcgcccc tgacaacagg agcgattcgc tatgacatcc cgacgagact ggcagttaca
     4261 gcaactgggc attacccagt ggtcgctgcg tcgccctggc gcgttgcagg gggagattgc
     4321 cattgcgatc ccggcacacg tccgtctggt gatggtggca aacgatcttc ccgccctgac
     4381 tgatccttta gtgagcgatg ttctgcgcgc attaaccgtc agccccgatc aagtgctgca
     4441 actgacgcca gaaaaaatcg cgatgctgcc gcaaggcagt cgctgcaaca gctggcggtt
     4501 gggtactgac gaaccgctat cactggaagg cgctcaggtg gcatcaccgg cgctcaccga
     4561 attacgggca aacccaacgg cacgcgccgc gttatggcaa caaatttgca catatgaaca
     4621 cgatttcttc cctcgaaacg actgatttac cagcggctta ccacattgaa caacgcgccc
     4681 acgcctttcc gtggagtgaa aaaacctttg ccagcaacca gggcgagcgt tatctcaact
     4741 ttcagttaac gcaaaacggc aaaatggcgg cgtttgcgat tacgcaagtg gtgctggatg
     4801 aagctacatt gttcaatatt gcggtcgatc ctgactatca gcgtcaggga ttgggaaggg
     4861 cgctgctgga acatctgatc gacgaactgg aaaaacgcgg cgtggcgaca ctatggctgg
     4921 aagtccgtgc ctcaaatgct gccgccattg ccctgtacga aagtttaggc tttaacgagg
     4981 cgacgattcg ccgcaattac taccccacca cggacggtcg cgaagacgcc atcatcatgg
     5041 cgttgccaat cagtatgtaa gacaaggtgg aataatgaag tgggactgga ttttctttga
     5101 tgccgatgaa acgctgttta cctttgactc attcaccggc ctgcagcgga tgtttcttga
     5161 ttacagcgtc acctttaccg ctgaagattt tcaggactat caggccgtta acaagccact
     5221 gtgggtggat tatcaaaacg gcgcgatcac ttcattacag cttcagcacg ggcggtttga
     5281 gagctgggcc gaacggctga acgtcgagcc aggtaaactc aacgaagcct ttattaatgc
     5341 gatggcggaa atctgcacgc cgctgccggg cgcggtttct ctgcttaacg ccattcgtgg
     5401 caacgccaaa atcggcatca tcaccaacgg ctttagtgcc ttgcaacagg tgcgtctgga
     5461 acgcacgggc ctgcgtgatt acttcgattt gctggtgatt tccgaagaag ttggcgttgc
     5521 caaaccgaat aagaaaattt tcgattatgc gctggaacag gcgggcaatc ctgaccgttc
     5581 acgcgtgctg atggttggcg acactgccga gtccgatatt ctcggtggca tcaacgccgg
     5641 gcttgcgacc tgctggctga atgcacacca tcgcgagcaa ccagaaggca tcgcgcccac
     5701 ctggaccgtt tcttcgttgc acgaactgga gcagctcctg tgtaaacact gattgcctcc
     5761 cccccgttga tgggtaaaat agccgcaatt tttcgttttc aacaagcgcg gcgcgatgcc
     5821 gcttactcaa gaagaaagaa ttatgacgtt gtctccttat ttgcaagagg tggcgaagcg
     5881 ccgcactttt gccattattt ctcacccgga cgccggtaag actaccatca ccgagaaggt
     5941 gctgctgttc ggacaggcca ttcagaccgc cggtacagta aaaggccgtg gttccaacca
     6001 gcacgctaag tcggactgga tggagatgga aaagcagcgt gggatctcca ttactacgtc
     6061 tgtgatgcag tttccgtatc acgattgcct ggttaacctg ctcgacaccc cggggcacga
     6121 agacttctcg gaagatacct atcgtaccct gacggcggtg gactgctgcc tgatggttat
     6181 cgacgccgca aaaggtgttg aagatcgtac ccgtaagctg atggaagtta cccgtctgcg
     6241 cgacacgccg atcctcacct ttatgaacaa acttgaccgt gatatccgcg acccgatgga
     6301 attgctcgat gaagttgaga acgagctgaa aattggctgc gcaccgatca cctggccaat
     6361 tggctgcggc aagctgttta aaggcgttta ccacctttat aaagatgaaa cctatctcta
     6421 tcagagcggt aaagggcaca ccattcagga agtccgcatt gttaaagggc tgaataaccc
     6481 ggatctcgat gctgcggttg gtgaagatct ggcacagcag ctgcgtgacg aactggaact
     6541 ggtgaaaggc gcgtctaacg agttcgacaa agagctgttc cttgcgggcg aaatcactcc
     6601 ggtattcttc ggtactgcgc tgggtaactt cggcgttgat catatgctgg atggcctggt
     6661 ggagtgggcg cctgcgccga tgccgcgtca gaccgatacc cgtaccgtag aagcgagcga
     6721 agataaattt accggcttcg tatttaaaat tcaggccaac atggacccga aacaccgcga
     6781 ccgcgtggcg tttatgcgtg tggtgtccgg taaatatgaa aaaggcatga agctgcgcca
     6841 ggtgcgcact gcgaaagatg tggtgatctc cgacgcgctg acctttatgg cgggtgaccg
     6901 ttcgcacgtt gaagaagcgt atccgggcga tatcctcggc ctgcacaacc acggcaccat
     6961 tcagatcggc gacaccttta cccagggtga gatgatgaag ttcaccggta ttccgaactt
     7021 cgcaccagaa ctgttccgtc gtatccgcct gaaagatccg ctgaagcaaa aacagctgct
     7081 caaagggctg gtacagcttt ccgaagaggg cgcggtgcag gtgttccgtc caatctccaa
     7141 caacgatctg atcgttggtg cggttggtgt gctgcagttt gatgtggtgg tagcgcgcct
     7201 gaagagcgaa tacaacgttg aagcagtgta tgagtcagtc aacgttgcca ctgcccgctg
     7261 ggtagaatgt gcagacgcga agaaattcga agagttcaag cgtaagaacg aaagccaact
     7321 ggcgcttgat ggcggcgata acctcgctta catcgctacc agcatggtca acctgcgcct
     7381 ggcacaggaa cgttatccgg acgttcagtt ccaccagacc cgcgagcatt aattcttgcc
     7441 tccagggcgc ggtagccgct gcgtcctgtc aatttccctt ccttattagc cgcttacgga
     7501 atgttcttaa aacattcact tttgcttatg ttttcgctga tatcccgagc ggtttcaaaa
     7561 ttgtgatcta tatttaacaa agtgatgaca tttctgacgg cgttaaatac cgttcaatgc
     7621 gtagatatca gtatctaaag ccgtcgattg tcattctacc gatattaata actgattcag
     7681 aggctgtaat ggtcgttatt catcactcat cgcttttgtg atggcgacca ttgacttctg
     7741 tagagggtga agtctctccc tattcagcaa tgcaacctcg tgttgccagg ctcaaattac
     7801 gagcaaacat acaggaataa atcgatgact atgacaagac tgaagatttc gaaaactctg
     7861 ctggctgtaa tgttgacctc tgccgtcgcg accggctctg cctacgcgga aaacaacgcg
     7921 cagactacca atgaaagcgc agggcaaaaa gtcgatagct ctatgaataa agtcggtaat
     7981 ttcatggatg acagcgccat caccgcgaaa gtgaaggcgg cgctggtgga tcatgacaac
     8041 atcaagagca ccgatatctc tgtcaaaacc gatcaaaaag tcgtgaccct gagcggtttc
     8101 gttgaaagcc aggcccaggc cgaagaggca gtgaaagtgg cgaaaggtgt tgaaggggtg
     8161 acctctgtca gcgacaaact gcacgttcgc gacgctaaag aaggctctgt gaagggctac
     8221 gcgggtgaca ccgccaccac cagtgaaatc aaagccaaat tgctggcgga cgatatcgtc
     8281 ccttcccgtc atgtgaaagt tgaaaccacc gatggcgtgg ttcagctctc tggtatcgta
     8341 gattctcagg cacaaagtga ccgtgctgaa agtatcgcca aagcggtaga tggtgtgaaa
     8401 agcgttaaaa atgatctgaa aactaagtaa ttcgtcgtaa ttcgtcctcc cgaactttgt
     8461 cgggaggcgc gatgtgcacc acactaaaaa tatcgcattg agtgattttc acgctcatat
     8521 caagcggtga cattaactat ggtaaaggag acgcttatgt ttcgttgggg catcatattt
     8581 ctggttatcg cgttaatcgc cgccgcactt gggtttggtg gtctggccgg taccgctgca
     8641 ggcgcagcta aaattgtctt tgtcgtcggg attattctgt tcctggtgag tttgttcatg
     8701 ggccgaaaac gaccctagat ttcagaccat taagaaaatt ttatccaaag ccagtccagc
     8761 ggactggctt ttgcggtttt agcgaataat aaattgcgtt actttgtcat tctttcacaa
     8821 caaaaacagg aaggcagagg tggggcagcg aatacctgta acgcttggta atattgcgcc
     8881 gttgtcgcta aggccgttcc agcctggacg aatagctctg gtgtgcgaag gcggcggaca
     8941 gcgtggaatt ttcacggctg gcgtgctgga tgagtttatg cgcgcgcagt ttaatccttt
     9001 cgatctttat ctcggcacat ctgccggggc gcagaacctc tcggcgtata tctgcaatca
     9061 gcccggttac gcgcgcaaag tcatcatgcg ctataccaca aaacgcgaat ttttcgatcc
     9121 attgcgcttt gtccgtggag gaaatcttat cgatctcgac tggctggtgg aggccaccgc
     9181 aagccagatg ccgttgcaaa tggataccgc cgcgcggttg tttgacagcg gcaaatcgtt
     9241 ttatatgtgc gcctgtcgtc aggatgacta cgcgccgaat tactttttac caaccaaaca
     9301 aaactggctg gatgtgattc gcgcctccag tgcgatacct ggcttttatc gtagcggagt
     9361 gtcgctggaa ggcattaact acctggatgg cgggatcagt gatgcgattc cggttaaaga
     9421 ggcggcaagg cagggcgcta aaacgttggt cgtcatccgc actgtgccgt cacaaatgta
     9481 ctacacgccg cagtggttca aacgcatgga acgctggctg ggtgacagta gcctgcagcc
     9541 gctggtcaat ctggtgcagc atcatgaaac cagctatcgt gacattcagc aatttattga
     9601 gaaaccaccg ggcaagctgc ggatattcga aatttatccg ccgaagccat tacatagtat
     9661 cgcgcttggc agtcggattc cggcgctgcg tgaagactat aaacttgggc gtttatgcgg
     9721 tcgttatttc ctcgccacgg ttggcaagct attaactgaa aaagcgccgc ttacccgcca
     9781 tctggtgcca gtggtgacgc cggaatcgat tgtcattccg cctgcgccag tcgccaacga
     9841 tacgctggtt gccgaagtga gcgacgctcc gcaggcgaac gacccgacat ttaacaatga
     9901 ggatctggct tgatttgccg ttttatcgac acccactgcc attttgattt cccgcctttt
     9961 attggcgatg aagaggccag cctgcaacgc gcggcacaag cgggcgtagg caagatcatt
    10021 gttccggcaa ccgaggcgga aaattttgcc cgcgtgcagg cgttagcgga aaattatcaa
    10081 ccgctgtatg ccgcattggg cttgcatcct ggtatgttgg aaaaacatag cgatgtgtct
    10141 cttgatcagc tacagcaggc gctggaaagg cgtccggcga aggtggtggc ggtgggggag
    10201 agcggtctgg atctcttcgg cgacgatccg caatttgaga ggcagcagtg gtttctcgac
    10261 gaacaactga aactggcgaa acgctacgat ctgccggtga tcctgcattc acggcgcaca
    10321 cacgacaaac tggcgatgca tcttaaacgc cacgatttat cgcgcactgg cgtggttcac
    10381 ggtttttccg gcagcctgca acaggccgag cggtttgtac agctgggcta caaaatcggc
    10441 gtaggcggta ctatcaccta tccacgcgcc agtaaaaccc gcgttgtcat cgcaaaatta
    10501 ccgctggcat cgttgttgct ggaaaccgac gcgccggata tgccgctcaa cggttttcag
    10561 gggcagccta accgcccgga acaggctgcg cgggtgtttg acgtgctttg cgagttgcgc
    10621 ccggaaccag aagatgagat tgccgaagtg ttgcttaata acacgtatgc ggtgtttaac
    10681 gttcgtgggt agtttgccct tcgtaggcag catccggcag tcggcgcata atgcctgatg
    10741 cgacgcttaa cgcgtcttat catgcctaca ggtttgtgcc gaaccgtagg ccggataagg
    10801 cgttcacgcc gcatccggca gttggcgcac aatgcctgat gcgacgcttg acgcgtctta
    10861 tcaggcctac aagtctgtgc cgaaccgtag gtcgtatccg gcatgtcaca aatacagcgc
    10921 cggaaatatt aaccggctca ccccgcgctc ccgtaacgca tcagccaacg gctcaacgtc
    10981 ttccggcgtg gcgctcgccc agctttgcgc ctcgccatac acgccgtggg catgaaacgc
    11041 gttcaggcgt accggaacat cgccgactcc cttgataaac gccgccagtt cttcgatgtg
    11101 ttgcaaataa tccacttggc cagggattac cagcaaacgc agttccgcca gcttaccgcg
    11161 ctctgccagc aaacagatgc tgcgcttaat ctgctgatta tcgcgtccgg ttaattgctg
    11221 atgacattcg ctcccccacg ctttgagatc gagcattgcg ccgtcgcaca ccgggagcaa
    11281 tttttcccag ccggtttcgc tcaacatacc gttactgtcc accaggcagg tgaggtggcg
    11341 cagttgcgga tcgtttttga tagcagtaaa cagcgccact acaaacggca gttgggtcgt
    11401 ggcttcgccg ccactcaccg ttatcccttc gataaacagc actgctttgc ggacatggct
    11461 aagcacctca tccacgctca tggattgcgc cattggcgtg gcatgttgcg gacacctctt
    11521 caggcaggta tcacattgct cgcagaccac agcgttccac accactttgc cgtcaacaat
    11581 ctgcaacgcc tgatgcggac actgtggcac gcactcccca cagtcattgc aacgtcccat
    11641 cgtccacgga ttgtgacagt ttttgcagcg cagattgcag ccctgcaaaa acagagccag
    11701 acgactgcct ggcccgtcaa cgcaggagaa ggggataatc ttactgacta aagcgcatct
    11761 gctgttcatg gcttatcacg cgcggctggc gttccagaat acgagtgttg cgtgcggctt
    11821 cttcgcccag ccaggtggtg ttggtgcgtg aaccttcggc gcgatatttt tctaaatccg
    11881 acaaacgcac catataaccg gtaacgcgaa ccagatcgtt accgctgaca ttggcggtaa
    11941 attcacgcat tccggcttta aaggcaccga ggcaaagctg taccaacgcc tgcgggttac
    12001 gtttgatggt ttcgtcgagc gtcagaatgt cgctgatgcc agaataataa taagcatgat
    12061 gcgggcgaca gtttgcagat gggtgatcgg atctggctca tcgccatacg gcaaacgcgc
    12121 gcccggcgtg gtgccgatat cggaactgat ccccgactgt gcgtgtaaca tggcgcgttt
    12181 ttgccagcca tatttcacgg gggtattggc gacaaactcc gccagctgtg cactgatgcg
    12241 ataacctact tcatttgcgg cggcttcttt accgtagcgc gcggcaatcc cttctttttc
    12301 acacagcaag ttaaccgctt ccgccagccc atacatgcca aacattggca caaaacgttc
    12361 agggttaatc agcccttctt tcaccaggaa gctattctca aagaagtgtg attgttgata
    12421 gaggaattca caccgcgcat cgatgatggc gatctgctgc tggcagtagt gcggtagagt
    12481 gcgcgtaaag aagtcatcca gcgattcgct gcgctcggca atggctttca ggttaaggcg
    12541 taccagcgtg ctgccaccac ccgccagcgg cagtgagttg taacagctca caatcccgta
    12601 gccccctttt gtgaaaattt tatcatgcac cggaccgttg gcgatgtgcg gtttgctaca
    12661 ttcacagatg ttcttcgcca cttccagcag caggtcatca ggggtgattt caggatcgta
    12721 gataaaggtc aggttcggtg aaacctgctt caaatctgcg tctgcacgta agatcgcgcg
    12781 ggtaatgggc gaatcagacg ggccgatatt ggcgtgcata aaggcgtctg gcagggttct
    12841 gtcgaggtaa cgccagaaac gttttattcg aacatcgatc tcgtcttgtg ttagaattat
    12901 aacatacggt tgcaacaacg catccagttg ccccaggtag accggcatcg atgtgaccga
    12961 cggtacgtgg tggtaaagaa tggtcagcag agagagtgcg tcatcaagat ctttcgcgcc
    13021 ttccagctcc agccattcgg aaccgttcgc cagaaaacgg gcgtaatcgg gtaagacata
    13081 gcgcggtttg tacggcgcat gaccttcaaa catatcgcag attacacctt catccagcgc
    13141 gcggcgggct tcggcaggaa gctgtgggta aggcagattg ttttctgctt ccagtgccag
    13201 aaaatggcgc ttctgctccg ggctaagcac tgggctggtg acaatttgct ggcaacgttg
    13261 ttgcagtgca ttttcatgag aagtgggcat cttcttttcc ttttatgccg aaggtgatgc
    13321 gccattgtaa gaagtttcgt gatgttcact ttgatcctga tgcgtttgcc accactgacg
    13381 cattcatttg aaagtgaatt atttgaacca gatcgcatta tagtgatgca aacttgtaag
    13441 tagatttcct taattgtgat gtatatcgaa gtgtgttgcg gagtagatgt tagaatacta
    13501 acaaactcgc aaggtgaatt ttattggcga caagccagga gaatgaaatg actgatctga
    13561 aagcaagcag cctgcgtgca ctgaaattga tggacctgac caccctgaat gacgacgaca
    13621 ccgacgagaa agtaattgct ctgtgtcatc aggccaaaac tccggtcggc aataccgccg
    13681 ctatctgtat ctatcctcgc tttatcccga ttgctcgcaa aacactgaaa gagcagggca
    13741 ccccggaaat ccgtattgct acggtaacca acttcccaca cggtaacgac gacatcgaaa
    13801 tcgcgctggc agaaacccgt gcggcaatcg cctacggtgc tgatgaagtt gacgttgtgt
    13861 tcccgtaccg cgcgctgatg gcgggtaacg agcaggttgg ttttgacctg gtgaaagcct
    13921 gtaaagaggc ttgcgcggca gcgaatgtac tgctgaaagt gatcatcgaa accggcgaac
    13981 tgaaagacga agcgctgatc cgtaaagcgt ctgaaatctc catcaaagcg ggtgcggact
    14041 tcatcaaaac ctctaccggt aaagtggctg tgaacgcgac gccggaaagc gcgcgcatca
    14101 tgatggaagt gatccgtgat atgggcgtag aaaaaaccgt tggtttcaaa ccggcgggcg
    14161 gcgtgcgtac tgcggaagat gcgcagaaat atctcgccat cgcagatgaa ctgttcggtg
    14221 ctgactgggc tgatgcgcgt cactaccgct ttggtgcttc cagcctgctg gcaagcctgc
    14281 tgaaagcgct gggtcacggc gacggtaaga gcgccagcag ctactaagta agatgcttta
    14341 cgcctgatgc gctgcgctta tcaggcctac gagacgtatc tacccgtagg ccggataagg
    14401 cgtagacgca tccggcaaaa gccgcctcat actcttttcc tcgggaggtt accttgtttc
    14461 tcgcacaaga aattattcgt aaaaaacgtg atggtcatgc gctgagcgat gaagaaattc
    14521 gtttctttat caacggtatt cgcgacaaca ctatctccga agggcagatt gccgccctcg
    14581 cgatgaccat tttcttccac gatatgacaa tgcctgagcg tgtctcgctg accatggcga
    14641 tgcgagattc aggaaccgtt ctcgactgga aaagcctgca tctgaatggc ccgattgttg
    14701 ataaacactc caccggtggc gtcggcgatg tgacttcgct gatgttgggg ccgatggtcg
    14761 cagcctgcgg cggctatatt ccgatgatct ctggtcgcgg cctcggtcat actggcggta
    14821 cgctcgacaa actggaatcc atccctggct tcgacatttt cccggatgac aaccgtttcc
    14881 gcgaaattat taaagacgtc ggcgtggcga ttatcggtca gaccagctca ctggctccgg
    14941 cggataaacg tttctacgcg acccgtgata ttaccgcaac cgtggactcc atcccgctga
    15001 tcactgcctc gatcctggcg aagaaactgg cggaaggtct ggacgcgctg gtgatggacg
    15061 tgaaagtggg tagcggcgcg tttatgccga cctacgaact ctctgaagcc cttgccgaag
    15121 cgattgttgg cgtggctaac ggcgcaggcg tgcgcaccac cgcactgctc accgacatga
    15181 atcaggtgct ggcctccagt gccggtaacg cggttgaagt acgtgaagcg gtgcagttcc
    15241 tgacgggtga atatcgtaac ccgcgtctgt ttgatgtcac gatggcgctg tgtgtggaga
    15301 tgcttatctc cggcaaactg gcgaaagatg acgccgaagc gcgcgcgaaa ttgcaggcgg
    15361 tgctggacaa cggtaaagcg gcagaagtct ttggtcgtat ggtagcggca caaaaaggcc
    15421 cgaccgactt cgttgagaac tacgcgaagt atctgccgac agcgatgctg acgaaagcag
    15481 tctatgctga taccgaaggt tttgtcagtg aaatggatac ccgcgcgctg gggatggcag
    15541 tggttgcaat gggcggcgga cgccgtcagg catctgacac catcgattac agcgtcggct
    15601 ttactgatat ggcacgtctg ggcgaccagg tagacggcca gcgtccgctg gcggttatcc
    15661 acgcgaaaga cgaaaacagc tggcaggaag cggcgaaagc ggtgaaagcg gcaattaaac
    15721 ttgccgataa agcaccggaa agcacaccaa ctgtctatcg ccgtatcagc gaataacggt
    15781 atactgatct gatcatttaa atttgaagca ctgagtacgg agaacatatg aaacgtgcat
    15841 ttattatggt gctggactca ttcggcatcg gcgctacaga agatgcagaa cgctttggtg
    15901 acgtcggggc tgacaccctg ggtcatatcg cagaagcttg tgccaaaggc gaagctgata
    15961 acggtcgtaa aggcccgctc aatctgccaa atctgacccg tctggggctg gcgaaagcac
    16021 acgaaggttc taccggtttc attccggcgg gaatggacgg caacgctgaa gttatcggcg
    16081 cgtacgcatg ggcgcacgaa atgtcatccg gtaaagatac cccgtctggt cactgggaaa
    16141 ttgccggtgt cccggttctg tttgagtggg gatatttctc cgatcacgaa aacagcttcc
    16201 cgcaagagct gctggataaa ctggtcgaac gcgctaatct gccgggttac ctcggtaact
    16261 gccactcttc cggtacggtc attctggatc aactgggcga agagcacatg aaaaccggca
    16321 agccgatttt ctatacctcc gctgactccg tgttccagat tgcctgccat gaagaaactt
    16381 tcggtctgga taaactctac gaactgtgcg aaatcgcccg tgaagagctg accaacggcg
    16441 gctacaatat cggtcgtgtt atcgctcgtc cgtttatcgg cgacaaagcc ggtaacttcc
    16501 agcgtaccgg taaccgtcac gacctggctg ttgagccgcc agcaccgacc gtgctgcaga
    16561 aactggttga tgaaaaacac ggccaggtgg tttcggtcgg taaaattgca gacatctacg
    16621 ccaactgcgg tatcaccaaa aaagtgaaag cgactggcct ggacgcgctg tttgacgcca
    16681 ccatcaaaga gatgaaagaa gcgggtgata acaccatcgt cttcaccaac ttcgttgact
    16741 tcgactcttc ctggggccac cgtcgcgacg tcgccggtta tgctgcgggt ctggaactgt
    16801 tcgaccgccg tctgccggag ctgatgtctc tgctgcgcga tgacgacatc ctgatcctca
    16861 ccgctgacca cggctgtgat ccgacctgga ccggtactga ccacacgcgt gaacacattc
    16921 cggtactggt ttacggcccg aaagtaaaac cgggctcact gggtcaccgt gaaaccttcg
    16981 cggatatcgg ccagactctg gcaaaatatt ttggtacttc tgatatggaa tatggcaaag
    17041 ccatgttctg atggatttgg gcggagcgtt gactccgcct ttgttatgtc acaaaaagga
    17101 taaaacaatg gctaccccac acattaatgc agaaatgggc gatttcgctg acgtagtttt
    17161 gatgccaggc gacccgctgc gtgcgaagta tattgctgaa actttccttg aagatgcccg
    17221 tgaagtgaac aacgttcgcg gtatgctggg cttcaccggt acttacaaag gccgcaaaat
    17281 ttccgtaatg ggtcacggta tgggtatccc gtcctgctcc atctacacca aagaactgat
    17341 caccgatttc ggcgtgaaga aaattatccg cgtgggttcc tgtggcgcag ttctgccgca
    17401 cgtaaaactg cgcgacgtcg ttatcggtat gggtgcctgc accgattcca aagttaaccg
    17461 catccgtttt aaagaccatg actttgccgc tatcgctgac ttcgacatgg tgcgtaacgc
    17521 agtagatgca gctaaagcac tgggcattga tgctcgcgtt ggtaacctgt tctccgctga
    17581 cctgttctac tctccggacg gcgaaatgtt cgacgtgatg gaaaaatacg gcattctcgg
    17641 cgtggaaatg gaagcggctg gtatctacgg cgtggctgca gagtttggcg cgaaagccct
    17701 gaccatctgc accgtgtctg accacatccg cactcacgag caaaccactg ccgctgagcg
    17761 tcagaccacc ttcaacgaca tgatcaaaat cgcactggaa tccgttctgc tgggcgataa
    17821 agagtaattg tgtttcgctg aaaggcgatt gtcgtgttaa gccggagtgg gggactgctc
    17881 cggcttttta gtatctattc atcaa
//
LOCUS       DAZFAB010000094        17282 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZFAB010000094 DAZFAB010000000
VERSION     DAZFAB010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.094"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            117..371
                     /gene="bssS"
                     /locus_tag="ACHJWA_003595"
     CDS             117..371
                     /gene="bssS"
                     /locus_tag="ACHJWA_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFO4310116.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            486..1604
                     /gene="solA"
                     /locus_tag="ACHJWA_003596"
     CDS             486..1604
                     /gene="solA"
                     /locus_tag="ACHJWA_003596"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFO4310117.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDDLSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETADGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFSLSRFQ"
     gene            1652..1765
                     /locus_tag="ACHJWA_003597"
     CDS             1652..1765
                     /locus_tag="ACHJWA_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFO4310118.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            2026..2592
                     /gene="yceJ"
                     /locus_tag="ACHJWA_003598"
     CDS             2026..2592
                     /gene="yceJ"
                     /locus_tag="ACHJWA_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFO4310119.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            2596..3171
                     /gene="yceI"
                     /locus_tag="ACHJWA_003599"
     CDS             2596..3171
                     /gene="yceI"
                     /locus_tag="ACHJWA_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFO4310120.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(3213..4265)
                     /gene="trhO"
                     /locus_tag="ACHJWA_003600"
     CDS             complement(3213..4265)
                     /gene="trhO"
                     /locus_tag="ACHJWA_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFO4310121.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVKTFRAQLYAFDSALDGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            4490..5410
                     /gene="lpxL"
                     /locus_tag="ACHJWA_003601"
     CDS             4490..5410
                     /gene="lpxL"
                     /locus_tag="ACHJWA_003601"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFO4310122.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            5582..6808
                     /gene="mdtG"
                     /locus_tag="ACHJWA_003602"
                     /pseudo
     CDS             5582..6808
                     /gene="mdtG"
                     /locus_tag="ACHJWA_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
     gene            6891..7265
                     /gene="msyB"
                     /locus_tag="ACHJWA_003603"
     CDS             6891..7265
                     /gene="msyB"
                     /locus_tag="ACHJWA_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFO4310123.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(7266..7493)
                     /gene="yceK"
                     /locus_tag="ACHJWA_003604"
     CDS             complement(7266..7493)
                     /gene="yceK"
                     /locus_tag="ACHJWA_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO4310124.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(7666..10209)
                     /gene="mdoH"
                     /locus_tag="ACHJWA_003605"
     CDS             complement(7666..10209)
                     /gene="mdoH"
                     /locus_tag="ACHJWA_003605"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFO4310125.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(10202..11737)
                     /gene="mdoG"
                     /locus_tag="ACHJWA_003606"
     CDS             complement(10202..11737)
                     /gene="mdoG"
                     /locus_tag="ACHJWA_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFO4310126.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            12130..13287
                     /gene="mdoC"
                     /locus_tag="ACHJWA_003607"
     CDS             12130..13287
                     /gene="mdoC"
                     /locus_tag="ACHJWA_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFO4310127.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGTFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(13295..14776)
                     /gene="clsC"
                     /locus_tag="ACHJWA_003608"
     CDS             complement(13295..14776)
                     /gene="clsC"
                     /locus_tag="ACHJWA_003608"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309451.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFO4310128.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMSEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIVLKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(14718..15251)
                     /gene="ymdB"
                     /locus_tag="ACHJWA_003609"
     CDS             complement(14718..15251)
                     /gene="ymdB"
                     /locus_tag="ACHJWA_003609"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFO4310129.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(15346..15657)
                     /gene="ymdA"
                     /locus_tag="ACHJWA_003610"
     CDS             complement(15346..15657)
                     /gene="ymdA"
                     /locus_tag="ACHJWA_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFO4310130.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(15778..16110)
                     /gene="csgC"
                     /locus_tag="ACHJWA_003611"
     CDS             complement(15778..16110)
                     /gene="csgC"
                     /locus_tag="ACHJWA_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFO4310131.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(16169..16627)
                     /gene="csgA"
                     /locus_tag="ACHJWA_003612"
     CDS             complement(16169..16627)
                     /gene="csgA"
                     /locus_tag="ACHJWA_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFO4310132.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGGNHGGGGNNSGPNS
                     ELNIYQYGGGNSALALQADARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSA
                     TLDQWNGKDSHMTVKQFGGGNGAAVDQTASNSTVNVTQVGFGNNATAHQY"
     gene            complement(16668..17123)
                     /gene="csgB"
                     /locus_tag="ACHJWA_003613"
     CDS             complement(16668..17123)
                     /gene="csgB"
                     /locus_tag="ACHJWA_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFO4310133.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         4334 a         4384 c         4096 g         4468 t
ORIGIN      
        1 ccccccttta aaaattcggt gattaccctt atttattggt atactgaagt tgcttcagat
       61 aaaaacgcat tgaacctcga ataacgttgt ctagtaacac gaattagggg gccatgatgg
      121 aaaaaaataa tgaagtcatt cagactcatc cgctcgtagg gtgggacatc agcaccgttg
      181 atagctatga tgcgctgatg ttgcgtttgc actaccagac cccaaataag tccgagcagg
      241 aagggactga agttggtcag acgctctggt taaccactga tgttgccaga caatttattt
      301 cgatattaga agcaggaatc gccaaaattg aatccggtga tttcccggta aacgagtatc
      361 ggcgtcatta acatgacatg accatccacc caaaaggcac ctcaccggtg cctttaccat
      421 ttctgggttg tatatctgca ttcagttaat tactctgctt aaaccatgag agcagagaga
      481 aaaaaatgaa atacgatctc atcattattg gcagcggttc cgtaggcgct gccgccgggt
      541 attatgcaac ccgcgccggt ttaaacgtgc ttatgaccga cgcccatatg ccaccacatc
      601 aacacggcag ccaccacggc gatacgcgat taattcgaca tgcttatggt gaaggcgaaa
      661 agtatgtccc gctggtcctc cgcgcacaaa cgctgtggga tgacctctcc cgccacaacg
      721 aagatgatcc catttttgta cgctctggtg tcattaacct tggcccggct gactccgcat
      781 ttctcgccaa cgtcgcccac agcgccgaac agtggcaact caacgttgaa aagctcgatg
      841 cgcaagggat tatggcccgc tggccagaaa tacgcgtccc ggacaactac atcggcttat
      901 ttgagactga ttccggtttt ttgcgcagcg aactggcgat taaaacctgg atccaactgg
      961 cgaaggaagc gggctgtgcg caactgttca actgcccggt caccgcaatt cgtcatgacg
     1021 atgatggcgt aactattgaa acggctgacg gtgagtatca ggcgaaaaaa gcgattgtct
     1081 gcgcgggaac atgggtaaaa gacctgctcc cggagctgcc tgtccagccc gtacgtaaag
     1141 tatttgcctg gtatcaggcc gatggccgct atagcgtgaa gaataaattc ccggcgttta
     1201 ccggtgaact gcccaatggc gatcaatatt atggttttcc ggcagaaaac gacgcgttga
     1261 agattggcaa acataacgga ggccaggtta tccattcagc ggatgaacgt gttccgtttg
     1321 cggaagtggt cagcgatggt tcggaagcct tcccgttctt gcgcaatgta ttgccgggta
     1381 tcggttgctg cctgtacggc gctgcctgca cctatgataa ttcgcctgac gaagatttta
     1441 ttatcgatac cctacccggc cacgataata cactgctcat taccggcctg agtgggcacg
     1501 gttttaaatt tgcgtcagtt ttaggggaaa tagctgccga ttttgcgcaa gacaaaaaaa
     1561 gcgattttga tttgacgcca ttcagccttt cccgcttcca ataatcatca ttacggcctc
     1621 cagaatgagg ccgtttttac aggagtattt aatgcgtcgg ctgttgcact atctcatcaa
     1681 taatattcgc gagcatctga tgctatatct ttttctctgg ggattgttgg caattatgga
     1741 tctgatctat gtgttttatt tttagttttc cgcactgaca taaactgaca aatatttaat
     1801 ctccctttca tttagcgtga aaaattgaat gagattattc ctgcttctac agcactcacc
     1861 agacatctct gatgctgaac ccactaaact tatgaagatt ctttcattat taattcaaaa
     1921 ttattgaaat aaaaataaat atcattcaat ttgttatttt tgtgttgcaa ttcattctcc
     1981 gacacgccat tttctttctc tcacaaattc gttttggatg cacttatgtc attcacaaat
     2041 acccctgaac gctatggtgt tatatctgcg gccttccatt ggctaagcgc aattattgtc
     2101 tacggcatgt ttgctttagg tctgtggatg gtcacgctca gttattacga tggctggtat
     2161 cacaaagcac ccgaactaca taaaagcatc ggtattttgt taatgatggg gctggttatt
     2221 cgcgttctgt ggcgtgtcat atctccaccg ccgggtccgc tgccgagtta ttcgcccatg
     2281 actcgtcttg ccgcaaaagc tggacatctc gcattgtacc tattgctgtt cgccatcggc
     2341 atcagcggct atctgatctc aactgccgat ggtaaaccga tcagcgtttt tggctggttt
     2401 gacgtccccg cgacccttgc cgacgctggc gcacaggcag actttgccgg tgccctgcat
     2461 ttttggctcg cgtggagcgt cgtagtactg tccgttatgc acggatttat ggccctgaag
     2521 catcatttca tcgataaaga cgacactctg aagcgcatgc tgggaaagtc gtcatctgac
     2581 tatggagtat aaaaaatgaa aaaaagcctg cttggtttaa ccttcgcgtc cctgatgttc
     2641 tctgccggtt cagcggttgc cgccgattac aaaattgaca aagaaggtca gcacgccttt
     2701 gttaactttc gcatccagca tcttggctat agctggttat atggcacctt taaagatttc
     2761 gacggtactt ttacctttga tgaaaaaaat ccagctgccg ataaagtgaa tgtgacaatt
     2821 aacaccacca gcgtcgatac taatcacgcc gaacgtgata aacatcttcg cagtgcagat
     2881 ttcctcaata ccgcaaaata tccacaggca acattcacct ccaccagcgt gaagaaagac
     2941 ggtgacgaac tggatattac cggcgatctg acgctgaatg gtgtaaccaa acccgtcacg
     3001 ctggaagcga aattaattgg tcagggcgac gacccatggg gtggtaaacg tgcaggcttc
     3061 gaggccgaag gcaaaattaa gctcaaggac ttcaatatca agacagattt aggtccagct
     3121 tctcaggaag tggatctgat tatttcagtg gaaggcgtac agcagaagta agagtgaaat
     3181 gttgccggat ggcacgcatc cggcaatgat atttattctg ttggatcagg aatgcccagt
     3241 gttgtattca gacgtccacg agacttatta aagatcttat tgccattttc acgtcctgcc
     3301 cgacggcgtc gctgttcttc tggcggtaac gcgctttctt cgcagcaaat ttcactacaa
     3361 caacctttgt atttttccgc acatactgga cactgaataa acagcaggtg gcagccatca
     3421 tttttacagt tggtatggct gtcgcacggc gcaccgcact ggtggcaatg cgcgataatc
     3481 tcatcagata tacgttcgcc catccgctcg tcaaaaacaa aatttttgcc aataaaacgc
     3541 accggcaagc cctgctcgcg cgccttacgg gcgtattcaa taattccacc ctcgatatgc
     3601 cagactttat tgaatccgtt atgtttcatc caggcactgg ccttttcaca acgaatgccg
     3661 ccggtgcagt acatgacgat ttttttatct ttatgtgcct gcatcatctc gactgctttt
     3721 ggcagctgct cacggaaggt atctgccgga atttccagcg cgttttcaaa gtgccccact
     3781 tcatactcat agtggttacg catgtcgata aacagtgcat cgggatcgtc aagcatggcg
     3841 ttcacttccg ccgcttgcag atactcacca acattgctgg catcaaagtg aggatcgtca
     3901 ataccgtcgg caacgatacg atcgcggacc ttcatgcgca gtacccagaa ggatttcccg
     3961 tcatcatcca acgcgatatt caggcgtaag ccatctaaag ccgagtcgaa ggcatagagc
     4021 tgcgcgcgaa atgttttaac attgctcgca ggtacgctga tttgcgcgtt aatgccctca
     4081 tgcgccagat acactcgccc aaaaacattc agcgcggtaa acagctgata taaagcgtca
     4141 cgggtcgcct taggatcggc gatgtggaaa tacttgtaaa acgaaatggt ggttcgcggt
     4201 tcgctctcag ccaacatttt ggcttttaac gcgtcgttgg aaatgcggtt gtgtaacact
     4261 ggcatggtgt acggttcctg cgagatggga aagtaaaaat ccgcggcatg atatagcaat
     4321 tatcgataat taacatccac acattttacg ctacatttgc gcattaaaaa ttatttgtta
     4381 tttacaagcg cggcaatttc gcccagtctt cagccacaaa ttttggttgc gggcgaaaaa
     4441 atgcgacaat acatacaatt gcccgaatag gttgaaaaac aggattgata tgacgaatct
     4501 acccaagttc tccaccgcac tgcttcatcc gcgttattgg ttaacctggt tgggtattgg
     4561 cgtactttgg ttagtcgtgc aattgcccta cccggttatc taccgcctcg gttgtggatt
     4621 aggaaaactg gcgttacgtt ttatgaaacg acgcgcaaaa attgtgcatc gcaacctgga
     4681 actgtgcttc ccggaaatga gcgaacaaga acgccgtaaa atggtggtga agaatttcga
     4741 atccgttggc atgggactga tggaaactgg catggcgtgg ttctggccgg accgccgaat
     4801 cgctcgctgg acggaagtga tcggcatgga acacattcgt gacgtgcagg cgcaaaaacg
     4861 cggcatcctg ttagttggca tccattttct gacactggag ctgggtgcgc ggcagtttgg
     4921 tatgcaggaa ccggggattg gcgtttaccg cccgaacgat aatccactga ttgactggct
     4981 acaaacctgg ggccgtttgc gctcaaataa atcgatgctc gaccgcaaag atttaaaagg
     5041 catgattaaa gccctgaaaa aaggcgaagt ggtctggtac gcaccggatc atgattacgg
     5101 cccgcgctca agcgttttcg tccctttatt tgccgttgag caggctgcga ccacgaccgg
     5161 aacctggatg ctggcacgga tgtccggcgc atgtctggtg cccttcgttc cacgccgtaa
     5221 gccagatggc aaagggtatc aattgattat gctgccgcca gagtgttctc cgccgctgga
     5281 tgatgccgaa actaccgccg cgtggatgaa caaagtggtc gaaaaatgca tcatgatggc
     5341 accagagcag tatatgtggt tacaccgtcg ctttaaaaca cgcccggaag gcgttccttc
     5401 acgctattaa atctcccatg ccggatgctt cagaatggca tccggcatta ccacagcaaa
     5461 tccccctgat ttagcgataa aagctctctg gattgcgccc cctggaagtc gggcgcataa
     5521 ttagtgtgct tatcttttct tcttatgttc accgcgcctg gcgcaccaac agcggattgc
     5581 tatgtcaccc tgtgaaaatg acacccctat aaactggaaa cgaaacctga tcgtcgcctg
     5641 gctaggctgt tttcttaccg gtgccgcctt cagtctggta atgcccttct aacccctcta
     5701 cgttgagcag cttggcgtta ccggccactc cgccctgaat atgtggtccg gtattgtctt
     5761 cagcattaca tttttatttt cggccatcgc ctcaccgttt tggggtggac tcgccgaccg
     5821 taaaggccga aaactcatgc tattacgctc tgctctcggc atgggcatcg tgatggtgtt
     5881 gatggggctg gcacaaaata tctggcagtt tttgatcctg cgggcgcttc ttgggttact
     5941 tggcggattt gtccccaacg ctaatgctct tatcgccaca caagtaccgc gtaataaaag
     6001 cggctgggca ctgggtacgc tctccacagg cggcgttagc ggtgcgttgc tcggcccaat
     6061 ggctggcggc ctgctcgccg atagctacgg cttacgtccg gtattcttta ttaccgccag
     6121 tgtgctcata ctctgctttt tcgtcaccct gttttgcatc agagaaaaat tccagccggt
     6181 cagcaaaaaa gagatgctgc acatgcggga agtggtgaca tcacttaaaa acccgaaact
     6241 ggtactcagc ctgtttgtca ccacgttaat catccaggtg gcgacgggct caattgcccc
     6301 cattctgacg ctgtatgtcc gcgaactggc gggtaacgtc agtaacgtcg cctttatcag
     6361 tggcatgatc gcctcggtgc caggcgtagc tgctctgctg agtgcaccac gactcggcaa
     6421 acttggcgat cgaatcggac ccgaaaagat cctgattacg gcgctgatct tttctgtact
     6481 gctgttgatc ccaatgtctt acgttcagac gccattgcaa cttgggattt tacgtttttt
     6541 gctcggtgcc gccgatggtg cactactccc cgccgtacag acactgttgg tttacaactc
     6601 gagcaaccaa atcgccggac gtatcttcag ctataaccaa tcgtttcgtg atattggcaa
     6661 cgttaccgga ccattgatgg gagcagcgat ttcagcgaac tacggtttca gagcggtatt
     6721 tctcgtcacc gctggcgtag tgttattcaa cgcagtctat tcatggaaca gtctacgtcg
     6781 tcgtcgaata ccccaggtat cgaactgatt tttcgccttt catacttgca aaagcggaga
     6841 atcagctatc cttttccctg aaacctcatc aactcaaagg gagaatcgtg atgaccatgt
     6901 acgcaacgct tgaagaagcc attgacgctg cacgcgaaga atttcttgca gacaaccccg
     6961 gcatcgacgc cgaagatgcg aatgtgcaac agttcaatgc ccaaaaatac gttttgcagg
     7021 acggcgacat catgtggcaa gttgagtttt ttgccgacga aggggaagaa ggtgaatgtt
     7081 tacctatgct tagcggtgaa gccgcgcaaa gtgtttttga tggcgactat gatgagatag
     7141 agatccgcca ggagtggcag gaagagaata cattacatga atgggacgag ggggaatttc
     7201 agcttgagcc accgctggat accgaggaag gacgcgcagc agctgatgag tgggatgaac
     7261 gttaattact catacgggcc atgatgaatg tcgatcggca gcagtaaagt atcaaaaacc
     7321 agggagaatg gcagatcaag gattgtgaca taacgccagg cggagtcacg cacatcccat
     7381 tgcacaccgg gataatattg gttgccatgc ccctgccccg gtatagtgcg gctaatgatg
     7441 ctgccacagc cactcagcaa ggtcaccatg atgctcacca caattaaacg catttgatac
     7501 ctcttccgta aaaaaatacc ggcataacgc cggtattttt attggctcgt tgtttttgcc
     7561 agatgtggcg tgaacgcctt atccgaccta caaaatcttg caaaatcaac acattgcagg
     7621 agcgatgtag gcctgataag catagcgcat caggcaacta cgtttttatt gcgaagccgc
     7681 atccggttta cgcaatgcca gtggattgag ctttatccct tcgtaataac tcacccatga
     7741 agaatatctc tcaggggaat tccagacgcg gaaatgcaga cgggccatcg tcaccggatc
     7801 gcttagcagc acaaggcgac gatcgcgatt cagcttctct ggcgtctcgt tcagcgcctg
     7861 ttcaacatgg cggtcacggg cgatttccag caccttactg gcgcggtgac gcgcggtcgc
     7921 cattgcggtt gccagagcgt taaatgacgg gttaaacact gcgtgcataa aaccatcatc
     7981 aagggagcgt tgacggttca tctcaaggaa ccgatcggta tcaaccagta cctgcggcgg
     8041 tgaatactct tccgggatca ggaacagttt ccagcgtttg gtgcgcagac caacggtggc
     8101 acggctggaa atcaccgaaa caaacggtga caggatcaac gagaagacaa tcggtgccag
     8161 ccagaacagg aaacgcagat ccaaccacgc catcccgaca gcccacacta accctaacag
     8221 cagctgtgag ccgtggcgtt tgaacgcttc accccaggaa gtggagtcat catcacgctg
     8281 cggtgaattc cacaccactt cccagccaag gaacgcgctg acgacgaaga ccgtatggaa
     8341 cagcatgcgt accggagcca gcagcacgga aaacagcact tccagcagca acgataatgt
     8401 aacgcgccag aagccgccat attctttcgt tcctttacac caaataagca aaatgctcaa
     8461 tagctttggc agaaacaaca gcaccatggt cgaagcaaaa agtgcaatcg ccagctcagg
     8521 acgccactgc ggccacaccg ggaacaactg ccgtggttgc aggaagtatt gcggttcggt
     8581 caacgcatgc actacctgca atgcagtaga gagcgcgagg aacataaacc acagcggagc
     8641 ggagagataa gacatcacgc ccgtcaggaa taccgcacgg tgaaccgggt gcataccctt
     8701 caccaggaac agacggaagt tcatcaggtt accgtggcac cagcggcggt cacgttttag
     8761 ctcatcaagc aagttaggcg gtaattcttc ataagaaccc gggagatcgt aagcaatcca
     8821 gactccccaa cctgcacggc gcatcaacgc cgcttccacg aagtcatgtg acaggattga
     8881 accggcaaag gaaccttcgc ccggcagcgg ggccagtgca cagtgctcga taaacggttt
     8941 cacgcggata atcgcgttat gcccccagta gtgcgactcg ccaagttgcc agaagtgcaa
     9001 accggctgta aacagtggcc catacacgcg ggtcgcgaac tgctgacagc gtgcatacag
     9061 cgtatccata ccggacgctt tcggcgacga ctgaatgatc ccggcgttcg ggttggcttc
     9121 catcaggcgc accaggccgc acaaacaatc accggtcatt accgagtcag catccagcac
     9181 caccatatag ctgtactggc tgccccagcg acggcagaag tcatcgatat taccgctttt
     9241 acgcttcacg cgacggcggc ggcggcgata gaaaatctga ccttcgccac cgacttcagc
     9301 gataagctcc atccaggctt tttgctctgc gacgcagata tccgggttgt aactgtcact
     9361 cagaatgtag acatcaaagt gtttggcatt cccggtggct tttactgatt cccacgttgc
     9421 acgcaagcca gcaaaaacac ggttcacgtc ttcgttacag ataggcatga tcaacgccgt
     9481 gcgatgctcc gggtttaatg gttcatcgcc aactgttgac gcagatatac tgtatttatc
     9541 gcgaccaata agtagttgca ggaagcccat taacgccgtc cagaatccgg cggacaccca
     9601 acagaacagt accgcaaaga ggatcaggat accggtttgc agcatataag gcagaagctg
     9661 cataaaggaa acccacaaat cctgaccaac catatccata ggattaatca gcgcccaacc
     9721 ctgataagga agaatggtct tcatatacca ggtcgcgacg acagtttgcg cgagcgtcag
     9781 gatcaacaga atgtaacggc ggatggtacc gacggtacgc cacttttgct cgctctcctg
     9841 ctcttctttg gtcaaacgag ccagatagcg cggtgttaca tcgcgtccac gcaggcgatc
     9901 ccagaaacgg cctaccgggt tggtacgcca cgggtcggga aacatcgagg agcgttttgc
     9961 ttctggcatc gccttaagct gatcgcgccc ttcgtcatct ttaattaact gtccatcagc
    10021 aagtgaatct ggccaggctt gttccagacg cgcctttacc gagccttgcg gggagtcatc
    10081 ctcccgcgcc caggtgcggt gttcggcatc cagcgcctga tgaacggcgc ggatatcagt
    10141 cttcggcaat gccgctttct cgcttgcggc gatgggcatt gcgtcaatgt actcagttgt
    10201 cttattcatt ggcaggtaac tggtagctcc aggtttcact caacgtctga tcggcattca
    10261 ccagcgcagc gcgcatttca gtggttttct tggcatcttt cactttcaca cgcatcacca
    10321 gacgccagcc tttggtgacc gggttatagc gcaccgtgct ttcaactatc tcaccattat
    10381 caccaatgct ggtttgcgct gtgaccgggg tatcctctgg cagttttttc atctctgcgc
    10441 cggtaaaatc gaccacaaag gcgatagtac cgtcaggctg gcgaatcagg ttcgactgct
    10501 tcacatcccc cgttgaacga cgcgtttgtt gcacccatgc gttatctggc gcatgcagtt
    10561 tgtcttcatc acggctgaag gtgatggtgt atttaaagtt catctcttta cccggctccg
    10621 gcagctgatc cggcgtccag taagcgacga tgttatcgtt agtttcatcg ttggttggaa
    10681 tttccaccag ctcaacgctg cctttacccc actccccttt cggagtcacc catgcgcttg
    10741 gacggagatc gtaacgatca tcgagatctt caaagcggga gaaatcacga ccgcgctgca
    10801 acagaccaaa gccttgcggg ttttccatgg agaagctgct gaccgctaaa tgtttcgggt
    10861 tattcaacgg acgccagatc cattcgccat taccggcatg gatagagaga ccgttagagt
    10921 cgtgcaactc cggacgatag ttatttgctg gcgacggttg gttcggccca aacaggaaca
    10981 tactggttaa cggtgcaacc cccagtttgc cgactttatc gcgcagatag attttcgact
    11041 gcacatccac aaccgtgtca cgccctggca taactacgaa tttgtaagca cctgtcgcgc
    11101 gcggcgagtc aagcaatgca taaatggtta aacgtttatc agttggtttt ggacgctcga
    11161 tccagaactc tttgaagcgt ggaaattctt cacccgatgg caaggcggta tcaattgcca
    11221 gaccgcgggc agaaaggcca taaacctgac ctgcaccaat cacgcggaaa tagctggccc
    11281 cgagcatgct gacgatttca tcgtttttat ctttgctgtt gatcgggtaa agcactttaa
    11341 aaccggcaaa accaaggtct tttaccgtgt ctttgtcatg ctgaacatcg ccgaaagtga
    11401 aataatccgg gctgtatttg attcgtttga ctgcggtggc agtcacttca tttattttga
    11461 ccggggtatc gaagtacata ccctgatggt agaactcgag tttgaatggg gtcttcagat
    11521 tgttccagta cgctttgtca tgattaaact ggatctgctg atagtccgcg tatttcatat
    11581 cgcggaaaac ggagggcaag ttgcttttgg gcgcctcata gcctttcccg gctaaggatt
    11641 gagcttgctt tgcgacatca tcaatactga aagcccagct tgaagatgta tacagggtta
    11701 acattactgc agcactcaac caacgcattt tcatcatttg tagtttatgt ttcataatta
    11761 gtaagcactt ccccctttgt gtgcttatat cgatccgatc tattttaatg gataaccagc
    11821 cattccgaca acagaatccc ggtatcgttc agcacgctgg ctcatggatt aagcacctga
    11881 ttgcaaccgt tttcactttc cgtgcttctc ctaaagacag aagcatcgac ttcgtgtagg
    11941 gtttgccagc aaatttattt atcaaccgtc ttacagacgc ttccattctg gttagtggta
    12001 gcgatgatgc tatgcataaa aacttgaagc ctgcgcctgg cgcggtcagg tcggtgtcgc
    12061 tatggcaaca agcctaaagg cataacccga cagggataag acgaaaaatc cgagattagt
    12121 taaccatata tgaatccagt acccgcgcaa cgtgaatatt tcctcgactc catccgcgcc
    12181 tggctgatgt tgttagggat cccttttcat atttctttaa tctattcgag ccatacatgg
    12241 catgtgaata gcgccgaacc gtcattgtgg ctgacccttt ttaatgactt catccactcg
    12301 ttccgcatgc aggtattttt cgttatatcc gggtactttt cctacatgct ttttttacgc
    12361 tatcccttga aaaaatggtg gaaagtacgt gtcgaacgtg tagggatccc gatgttaaca
    12421 gccatccccc tactgacatt gccgcaattt attatgctgc aatacgtcaa agggaaagcg
    12481 gaaagttggc ctgggctgtc attgtatgac aaatataata cgttggcctg ggaattaata
    12541 tcacatctgt ggtttttact ggtgttagtg gtcatgacga cgctgtgcgt atggatattt
    12601 aaacgcatca gaaataattt agaaaattct gataaaacga ataaaaaatt ctcgatggta
    12661 aaactatcgg tgattttttt gtgcctcggc atcggttatg cggtaataag aagaacgatt
    12721 tttattgtgt atccacccat tctgagtaat ggcacgttca attttattgt catgcaaacg
    12781 ctattttatt taccgttctt tatcctcggc gcactggctt tcattttccc tcatcttaaa
    12841 gccttgttta ccacgccgtc tcgtggctgt accctcgctg cagcattggc gtttgtcgct
    12901 tatttactca accagcgcta tggcagtggc gatgcctgga tgtacgaaac cgagtcggtg
    12961 atcaccatgg tcctcggtct gtggatggtg aatgtggtct tctcctttgg ccaccgtttg
    13021 cttaacttcc agtcagcgcg ggtgacttat tttgttaacg catcgctgtt tatctatctg
    13081 gttcaccacc cgttaacgct gtttttcggc gcatacatta caccgcacat cacctccaac
    13141 tggcttggtt ttctctgtgg cctgatattc gtagtaggga ttgcgataat tctgtatgaa
    13201 attcatttgc gcatcccgtt actgaagttt ttgttctctg gtaaaccggt tgttaagcgt
    13261 gagaacgata aagcaccagc ccgttaagcc acatttacaa taaccattcc acgggcaata
    13321 tcgacgccag tctgaccata acccgcttcc agaaactggt agcgggttct tttttgagaa
    13381 caatctcttt ctttgcatga cgatcaaccc agttgatccg tccccacctg tccagacgga
    13441 gctgccaggc cgcatcatac tggctctgaa taaagcgttt atcaattaac tgagccagcg
    13501 tttcgctctc tatcacgaag cccatttcag tattgagcaa tgttgaacgc ggatcgaaat
    13561 tgaaagaacc gataaacacc gttttaccat cgatgctaaa ggttttagca tgcaggctgg
    13621 caccggaatt accggttatg ccgcgatcgt gtaacgtact actttgttca cgcgtcggct
    13681 tgagttcata taattccacg ccatagcgga gcaatttttt gcgccagcgc gcgtatccgg
    13741 catggacgac agcaacatcg ttagcggcaa gagaattggt taggatcgca atctttaccc
    13801 cttttctcac catccgtaag agttgcgcca cacctgcgcg tgtcggtaca aaataggaag
    13861 agataatatc gatgcgttca ctgggtgagc ccatgatatc gaacaggcgc tgcggtaaca
    13921 gtgaatgccg ttttgccttg ccctcccctt tcgccggatc atcacttaat aaacgtgttt
    13981 tcgcccagat aagcggcaac gttccatcaa ccagatgatt tataaacgga ctggattcca
    14041 ttttgcgtaa gtaacgatgc gtcatggcat cgttatgcca ggaggcgggt aactcgatgc
    14101 gatccgccat ttcaccttcc gggacatcca gcacctgctg taagggtgaa accgatttgc
    14161 aataccagta gcgggcgaaa tcatcggcaa cgtcctctac tacgggtcct attgccatga
    14221 catctaaatc cgaaaaaagt ggctcttccc ctgccccaaa ataggcatca ccaatatttc
    14281 gcccccctac caacgtcacc acgccatcga cagtgaaact tttattgtgc atacggcgat
    14341 tgagacggga aaagtcggtg atataaccaa gcggacgcag caagcgaaac gagaaaggat
    14401 taaaaagccg gacttcaatg cggggatggc tgtcaagcaa gcgtaaaatg tcgtcaagtc
    14461 cgggcgtatt gttgtcgtcc agcaacaaac ggacgcgaac gccacgcttt gctgcggcta
    14521 acagggcgga aaacagtaac cgacccgaca tatcgtcctg ccagatgtaa tactgaacat
    14581 ccagcgtatg ctctgacatt tcggccagac gataccgggc ggcaaacgca tccagacttt
    14641 tctccagagg gaaaagacca cactgaccgg gatgttgcga acacagtggc agcaccgcgc
    14701 tcgccagccg gggcaaatca ttcatctcct tgttgggtaa ggagtctttc gtagaggtgg
    14761 gcgttttctt catcataaca gacaaagtat acctgttcgg gtaaagcgtg acgggtaata
    14821 aattctgaaa cggtttttac tgcgatttca gccgctgccg cacgagggta accataaacc
    14881 ccagtactga ttgcaggaaa agccactgac gtatagctgt ttgccgccac cagtcgtagg
    14941 ctattgagat aggcatcctg caaaagctgg tcttcgtttt gttcaccacc acgccagact
    15001 ggcccgacgg tgtgcactac ggctttagca ggaagatcgc ctgcaagcgt aataacggca
    15061 tgccccgtag ggcaatcgcc ctgctgttgc ctgactttta aacaagcatc cagcagggcc
    15121 ggacccgctg cgcgatgaat ggccccatcg acgccgccac ctcccattaa tgaaggatta
    15181 gccgcattca caatcacatc aacggccagt ttggtaatat caccctgcac aacatgaata
    15241 cgcgttttca taacgcctcc ttacaccttc ttgtttctta agtgtacagc aggctttttg
    15301 gttaaatcgc atttttctgc tcagcaacgt gagttgttca gaaacttagt tatattcgat
    15361 gttcattaca gccagctttt tctgtttatc aagatactgc acttttactg aggctatttg
    15421 cgcgtttttg acattgccgc tcattaacgc tttgctggag taggttttac ccggaacaga
    15481 accattctgt gggcagttca tttctacggg tgatgactgg gtgctgacgt cacatgccgg
    15541 ttccacaatt tggccataaa aatggatcac gcccccttgc gcggtgcttc ctgcgatggc
    15601 aataaaagga aaaaacaaac tgccgagcat aagagagttt aaaaaaggtc tgaacattta
    15661 ttctatttcc tcaatgatta gtcatccttg agggttgtgt tatccatact ttcaggttgg
    15721 ctgattcgca gcagaccatt ctctccagat tcatcttatg ctcgatattt caacaaatta
    15781 agacttttct gaagagggcg gccattgttg tgataaatga agtgactgtc catcagaaac
    15841 agtaacaact attttcaccc gatcgtccgg ggaaatattt aaactcaact tcgtcaaagc
    15901 aatgggttga ttagcaggca atgaaagggt cttttcttgc ttcgtctgac tttgccctga
    15961 actgccttcg cgcagggaca atatttgtac tctgcacaga caagattgag taagagtgac
    16021 ttcaggaata atggtgtaca tatccccttg ctgggtcgta ttaaaggtta tctgactgga
    16081 aagtgccgca aggagtaata acgcattcat attcttctcc cgaaaaaaaa cagggcttgc
    16141 gccctgtttc tttaatacag atgatgtatt agtactgatg agcggtcgcg ttgttaccaa
    16201 agccaacctg agttacgttg acggtggaat tagatgcagt ctggtcaacc gctgcaccgt
    16261 tgccgccacc gaattgttta actgtcatat gagagtcttt accgttccac tgatcaagag
    16321 tggcgctgtt accaaagcca cgttgggtca gatcgattga gctgtcatct gagccctgac
    16381 caacatctgc accgttacca ccaccatgct gggtaatagt aagatcagag ttacgagcat
    16441 cagcttgcag agcaagtgca gagttaccac caccgtactg ataaatattc agctctgaat
    16501 tcgggccgct gttattaccg ccaccaccgt ggttaccgcc accgccgccg tactgaggaa
    16561 caacacctgc cagagcgcta ccggagaata cgattgctgc aattgctgct acttttaaaa
    16621 gtttcatgta aaacccccat cggattgatt taaaagtcga atggaaatta acgttgtgtc
    16681 acgcgaatag ccatttgcga ctgtctctgc actacaattg ccgttttttg agtaccatac
    16741 tgtgtaatat ttgctttatt accagaacct ttctggataa tcatcgcagt attaccataa
    16801 gcaccttgcg aaatactggc atcattggca ctgcccgcct gatcaatata tgcaaggtta
    16861 taatctcctg tctggtcaat ctttgcccgg ttgctactac cttcttgcgc aacaaccgcc
    16921 aaaagttttg agcctccctg ccgtaactga gcactattat tagtcccagc ttgaccaatt
    16981 atggctgcct gattaaatga agacttactc aattcattta ccgcgaagtt atattctgaa
    17041 ttagctaaat cataacctgc tgcggctgca atcccaggcg cacccagtat tgttaacatc
    17101 ataaataaca atttgttttt catgttgtca ccctggacct ggtcgtacat ttaagaaatt
    17161 aaatcatttc aacttggttg ttaacgcaac ctatattttg ttaacgctgc gttacgatgg
    17221 aaagtatgtc tgcggaaata tttttaataa ctcacccgcg cgttgtattt ttcttttttt
    17281 gt
//
LOCUS       DAZFAB010000095        16741 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZFAB010000095 DAZFAB010000000
VERSION     DAZFAB010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.095"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..245)
                     /locus_tag="ACHJWA_003614"
     CDS             complement(<1..245)
                     /locus_tag="ACHJWA_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFO4310134.1"
                     /translation="MILSTRISLGLFFKYFLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNENTNV"
     gene            complement(392..1675)
                     /gene="yeaH"
                     /locus_tag="ACHJWA_003615"
     CDS             complement(392..1675)
                     /gene="yeaH"
                     /locus_tag="ACHJWA_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFO4310135.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            complement(1788..3722)
                     /gene="yeaG"
                     /locus_tag="ACHJWA_003616"
     CDS             complement(1788..3722)
                     /gene="yeaG"
                     /locus_tag="ACHJWA_003616"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFO4310136.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            4158..4904
                     /gene="mipA"
                     /locus_tag="ACHJWA_003617"
     CDS             4158..4904
                     /gene="mipA"
                     /locus_tag="ACHJWA_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFO4310137.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNSNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPIVDKSWTGLISTGITYKF"
     gene            4994..5848
                     /gene="yeaE"
                     /locus_tag="ACHJWA_003618"
     CDS             4994..5848
                     /gene="yeaE"
                     /locus_tag="ACHJWA_003618"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFO4310138.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAVMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELAMLDKAYPAPK
                     GKTALDMV"
     gene            complement(5899..6783)
                     /gene="yeaD"
                     /locus_tag="ACHJWA_003619"
     CDS             complement(5899..6783)
                     /gene="yeaD"
                     /locus_tag="ACHJWA_003619"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFO4310139.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDANGVALTFELTQSEETKKFWLHDFTLLAHFRVDKNCEIDLEAHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASEMQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(6867..7862)
                     /gene="gapA"
                     /locus_tag="ACHJWA_003620"
     CDS             complement(6867..7862)
                     /gene="gapA"
                     /locus_tag="ACHJWA_003620"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO4310140.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            8204..8617
                     /gene="msrB"
                     /locus_tag="ACHJWA_003621"
     CDS             8204..8617
                     /gene="msrB"
                     /locus_tag="ACHJWA_003621"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFO4310141.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            8659..8931
                     /gene="yeaC"
                     /locus_tag="ACHJWA_003622"
     CDS             8659..8931
                     /gene="yeaC"
                     /locus_tag="ACHJWA_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFO4310142.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARNNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            9301..10377
                     /gene="ydjL"
                     /locus_tag="ACHJWA_003623"
     CDS             9301..10377
                     /gene="ydjL"
                     /locus_tag="ACHJWA_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO4310143.1"
                     /translation="MKALARFGKAFGGYKMIDVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            10404..11783
                     /gene="ydjK"
                     /locus_tag="ACHJWA_003624"
     CDS             10404..11783
                     /gene="ydjK"
                     /locus_tag="ACHJWA_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4310144.1"
                     /translation="MDQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMGVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRHTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLIAM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGTPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            11800..12843
                     /gene="ydjJ"
                     /locus_tag="ACHJWA_003625"
     CDS             11800..12843
                     /gene="ydjJ"
                     /locus_tag="ACHJWA_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HFO4310145.1"
                     /translation="MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVH
                     GFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCL
                     EGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGTLVEPAAVGMHA
                     AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATEIAVVDVLEKRLAMAEQLGATVVI
                     NGAKEDTIARCQQFTEDMGADIVFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGASAI
                     NFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYDYRDVQQAFEESVNN
                     KRDIIKGVIKISD"
     gene            12864..13700
                     /gene="ydjI"
                     /locus_tag="ACHJWA_003626"
     CDS             12864..13700
                     /gene="ydjI"
                     /locus_tag="ACHJWA_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFO4310146.1"
                     /translation="MLADIRYWENDATNKRYAIAHFNVWNAEMLMGVIDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIKLFGSDGK
                     AE"
     gene            13705..14637
                     /gene="ydjH"
                     /locus_tag="ACHJWA_003627"
     CDS             13705..14637
                     /gene="ydjH"
                     /locus_tag="ACHJWA_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO4310147.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMTTGGDA
                     INEATIISRLGHRTALMSRIGKDAAGQFILDHCRKENIDIQSLKQDVNIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFSQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMNICADMIKPRLNETLDDICEALSYVNYAEAKLLTGKETLDEIADSFLACGVKT
                     VVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNLRECAR
                     FANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            14647..15627
                     /gene="ydjG"
                     /locus_tag="ACHJWA_003628"
     CDS             14647..15627
                     /gene="ydjG"
                     /locus_tag="ACHJWA_003628"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HFO4310148.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTAPGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGFDYIDIYMTHWQSVPPFYTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEVLGR"
     gene            15764..16522
                     /gene="ydjF"
                     /locus_tag="ACHJWA_003629"
     CDS             15764..16522
                     /gene="ydjF"
                     /locus_tag="ACHJWA_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO4310149.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            16640..>16741
                     /locus_tag="ACHJWA_003630"
     CDS             16640..>16741
                     /locus_tag="ACHJWA_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310150.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLT"
BASE COUNT         4378 a         3740 c         4208 g         4415 t
ORIGIN      
        1 acatttgtgt tttcgttata taaggtagta atgcaaaata aaattaacgt cagcaataaa
       61 ctgaaaataa aaaagctttg agcttttaat ttttgtgtac ccatagaaac cataaaaagg
      121 agagcaatgt gagtgctgga ttttattgat ggcagagata tatagttttg accaggatca
      181 attttcgtta acgataaaaa atatttaaag aataacccca gggaaatacg tgttgacaga
      241 ataatttttc cgaatgattg ttttctggaa ctaaattgtg taaaggttat gctgtcatat
      301 ttaaagcgat tgtaagctaa tgtatgtaat aaatgagata acttacagtg agctaaaaat
      361 cagccaggcg ataatgaccc ggctgatagt tttagtcttt agctgttgca ttttgcttat
      421 gaaacagttc acggaacacc ggataaatat catcctggtc gcggatatgc tgcatcgcaa
      481 agttgtcgaa agtagattgc agatgctcat attctcgcca cagtgtctga tgcgcgcgac
      541 gggtaatttc gatatagctg taataacgaa caacgggtaa taattttttc gccagaattt
      601 catggcaaag cggagagtca tcggcccagt tatcgccgtc cgatgcttgt gcagcgtaaa
      661 tattccactg tgccgggtta taacgctctt tcactacctc atccatcagt ttcagggcgc
      721 tggaaacaat ggtgccgcca gtttcctgcg agtagaaaaa ctcatgttca tcgacttctt
      781 tcgcctgggt atgatggcgg atgtatacga cttccacgtt cttatacgtt ctgctgagga
      841 acagatacag cagaatataa aaacgcttag ccatatcttt agtggattga tccattgaac
      901 cggaaacgtc catcaggcaa aacatcactg cctggctgga gggatcgggc cgcttctcgt
      961 agttcttgta acgtaaatcg aaggtgtcaa taaaagggac gcgttcaatt ttggcacgta
     1021 attctgcaat ttctttgcgc agacgttcct cttccagcag ctgcgcaggt tcactgttgc
     1081 tgatgatggc caaattctct tccagtgcat gaagttcccg ccgcttgcct gccgtcatgg
     1141 ctgtgcgtcg cgccagtgag ttctgcaagg aacgcacaac gctgatattg gccggaacgc
     1201 cgttagcggt ataacccgcc cgatgcgttt tatattcggt cagctggcgt tgttggtttt
     1261 gtttcagatt cggtaaggcc aaatcttcaa agagcagatc aagatactca tctttcgaaa
     1321 tctgaaagac aaattcatcc tgaccttcac catcctggct ggcctggccc tgaccactgc
     1381 cggaacctcc gccgccaccc tggggacgtt caattcggtc gttctgaacg aaatggtcat
     1441 tgcccggatg cacgcggtgg cgcagaccgc cacgcccctg atgaaacatt ggttcgctaa
     1501 tatcttccgt gggaatggat acggactcgc cgctgtcgac gtcagtcacc gaacgcttat
     1561 taatggcctc ggagatcgac tgtttaattt gcgctttata acggcgtaaa aaacgctggc
     1621 gattcaccat gcttttgttt ttgccgttca gacgccggtc aataaaccag gtcatatgcc
     1681 ccccgtacta catttgccaa ctgtatgcta cgcctgtgtt gccatttctc tggcccacgg
     1741 ttcgctgtcg ttgtaggccc ggtagcatgt gtaccgggcc agggttgtta agacgattta
     1801 cgtacgcgca aataccattc gcacagtaaa cgcacctgtt tacgggtgta gcctttttcc
     1861 atcatacggt cgacaaagtc gtcgtgtttc ttctgctcgt cggttgacgt tttggcgtta
     1921 aacgagataa ccggcaacag ctcctcggta ttggagaaca ttttcttctc gataaccgtg
     1981 cgcagttttt catagctggt ccagttcgga ttgcgtccgc tgttattggc tctggcgcgc
     2041 agtacgaagt taacaatctc gttgcggaaa tcttttggat tactgatccc cgccggtttc
     2101 tcgattttct ccagctcggc gttaagagac tcgcggtcaa acagctgccc ggtatccgga
     2161 tcgcgatact cctgatcctg aatccagaaa tccgcgtagg taacataacg gtcgaaaatg
     2221 ttttgcccat attcggaata ggattcaagg taggccgtct ggatctcttt gccaataaac
     2281 tcggcatatt tcgggatcag ataacctttc aggaactcca gatagcgttc tgcctgctct
     2341 tgtgggaact gctcgcgctc gatctgctgt tccaggacgt agaacagatg gaccgggttt
     2401 gccgccactt ctacatgatc gaagttgaac acgcgggaga ggatcttaaa cgcaaaacgc
     2461 gtcgacagac cgttcatacc ttcatcgaca ccggcgtagt cacgatattc ctgatacgac
     2521 ttggctttgg gatcggtgtc tttcagactt tcgccatcat aaacccgcat ctttgaataa
     2581 atgctggagt tttctggctc tttcaggcgc gaaagaatgg aaaaacgtga cagtgtttcg
     2641 agagtgccag gggcgcatgg ggcgtgagtc aattcactgt gattaagcaa tttctcgtag
     2701 attttgatct cttcggaaat gcgcaagcaa tacggcacct tcacgatgta aacacgatcg
     2761 aggaaggctt cgttgttttt gttattacgg aaagtgaccc attcggactc gttcgagtgg
     2821 gcaagaataa tcccgttgaa cggcagggcg gagatacctt ccgtcccgtt gtagttacct
     2881 tcctgagtgg cggttaacaa gggatgcagc acttttatcg gtgctttaaa catctcgaca
     2941 aattccatga tcccttggtt ggcgcggcac agcgcaccgg aatagccgta ggcgtccggg
     3001 tcattctgcg cgtaatgttc gagtttacga atatcgactt tcccaaccag cgcggagatg
     3061 tcctggttgt tctcatcacc gggttccgtt ttggcgatag caatttgttg cagaattgac
     3121 ggccagacct tcactacccg gaacttagtg atatcgccac caaattcatg caggcgtttt
     3181 gccgcccacg gcgacatgat ggtgccgaga taacggcgag gaatgccata ctctttttcc
     3241 agaatctgcg catcttcctg cggattgaaa agacagaacg gatgatcgtt gaccgggcta
     3301 cgctcaccgt tggcgctcaa tacataaatc ggtacgagct gcattaatga tttcagtcgc
     3361 tcagcaagcg atgatttacc cccacccaca ggccccagca gatacaggat ttgtttcttc
     3421 tcttccagcc cctgagccgc gtgtttcaga taagagacaa tctgttcaat cgcgtcttcc
     3481 atgccgtaaa actcttcaaa cgccggataa cgtgcaatga cccggttaga aaagagtcga
     3541 gaaagtctgg gttcctgggc tgtatcgacc atgacaggct caccgatagc catcaatagc
     3601 cgctcagccg cgttggcata agcactgcga tcttgccgac aagtggtaag aaactcctgc
     3661 agtgtgaact cttcgtcctt ggcagcttca tatcgctggc gatagtgatc gaatatattc
     3721 atggtatgcc gtcctttcgt tttttagcac aggttaagag ccgttcatat gaatagtaga
     3781 ggctcccgga agaggtaaac tgaacgacgt ctcttgctaa aacacagcaa ctctcatgcc
     3841 attttatcga cgatgagaac aacgtgcccg gtgatacacc ttcaaaatta agcgtagatg
     3901 gcaaatggaa aacttgccta cacggctttc ttaatttcaa tggcatatca acaactcatc
     3961 cataaatttt gcataattaa tgtaaagacc aggctcgcca gtaacgctaa attcatttgg
     4021 ctgtaagcgc ggtgtcatcc gcgtcaggaa aattaaacag ttactttaaa aaatgaaaac
     4081 gtaaaaaggt tgggtttcga tgtattgacg ggtaaacttt gtcgcccgct aaacatttgt
     4141 ttaaggaatg attaattgtg accaaactca aacttctggc acttggagtg cttatcgcaa
     4201 cgtctgcagg cgtagcgcac gctgaaggta aattttccct gggcgcaggc gtaggtgtcg
     4261 ttgaacaccc atataaagat tacgataccg atgtttaccc agtaccggta atcaactatg
     4321 aaggcgataa cttctggttc cgtggcttag gtggtggtta ctacctgtgg aatgacgcaa
     4381 cggataaact ttcaattacc gcttactggt cgccgcttta cttcaaagct aaagacagtg
     4441 gcgatcacca aatgcgtcac ctggatgacc gtaagagcac catgatggct ggtctgtctt
     4501 atgctcactt tacccagtac ggttacctgc gtaccaccct ggctggcgat accctggata
     4561 acagcaacgg catcgtctgg gatatggcct ggttgtatcg ttacaccaac ggtggcctga
     4621 ccgtgactcc gggtattggt gtgcagtgga acagcgaaaa ccagaacgaa tactattatg
     4681 gcgtatcgcg caaagagtcc gctcgcagcg gtctgcgtgg ctataactcg aacgacagct
     4741 ggagccctta cctggagctg agcgccagct acaacttcct cggcgactgg agtgtttacg
     4801 gtaccgcgcg ctacacccgt ctgtctgatg aagttactga cagcccgatt gtggataaat
     4861 cctggactgg cctgatttct accgggatca cctacaaatt ctgataatgc atctttccag
     4921 ggcattttgt gcataaaaac agggcgtcac gccctgtttt gcattacggt ggtgcaatac
     4981 ctgggaggaa acaatgcaac aaaaaatgat tcaatttagt ggcgatgtct cactgccagc
     5041 catagggcag ggaacatggt atatgggcga agatgccagt cagcgcaaaa cagaagttgc
     5101 tgcactacgc gcgggcattg aactcggttt aaccctcatt gataccgccg aaatgtatgc
     5161 cgatggcggt gccgaaaagg tggttgggga agcattaacc ggtctgcgag agaaggtctt
     5221 tctcgtctct aaagtctatc cgtggaatgc tggcgggcaa aaagcgataa atgcatgcga
     5281 agccagttta cgccgtctca atactgatta tctcgatctc tacttattac actggtctgg
     5341 cagtttcgct tttgaagaga ctgttgcagt gatggaaaaa ttgatcgccc agggaaaaat
     5401 ccgccgctgg ggcgtttcga accttgatta tgctgatatg caggaactct ggcagctgcc
     5461 ggggggaaat cagtgtgcca ctaatcaggt gctttaccat ctcggttcac gagggattga
     5521 gtacgatcta ctcccctggt gccagcaaca gcagatgccg gtgatggctt acagtccgtt
     5581 agcccaggcc gggcggttgc gcaatggact gttaaaaaac gcggtagtca acgaaattgc
     5641 acatgctcac aatatcagcg cggcgcaagt attgttggcg tgggtaatca gccatcaggg
     5701 tgtgatggcg attccaaaag cggccacgat tgcccatgtc caacagaatg cggctgtgct
     5761 tgaggtcgaa ctttcgtcag cggaattagc catgctggac aaagcgtatc cggcaccaaa
     5821 aggaaaaact gcgctggata tggtgtgatg ttgatacgtg ccggatgcat cgcacccggc
     5881 acacaacatt gacgcaaatt aacgtttggc aacgcgaatg gattgcgcca gatgtgcagg
     5941 tttttctttg gtcacttttt gcatttctga ggcgtaagct gtttctacac aaacaaaggt
     6001 cttgtagcca tcgtccggca tgtcgcccat gctaactgaa agcgccggtc ccgggttcca
     6061 gcctacaacg ttcagatgat gctggtggcc tacggcgata atacgattca gcgcttcatc
     6121 attaatcacg ctgcaatctt gcggattcag atacacgcga tcggtacggt cagggaaggt
     6181 ctgaataccg tcggtcagta catcttcttt cgcgtcattt actttatcaa tgaagcgatc
     6241 gcccagccca ctgacgctta ccttagcgat atcacccacg ttaaagtagg tatgcagggc
     6301 agaggtggtt tcaaattcgc cgtgtgcttc aagatcgatt tcacagtttt tatccacgcg
     6361 gaaatgcgct aacagagtaa agtcgtgcag ccagaatttt ttcgtctctt cgctttgtgt
     6421 caattcaaaa gtcagcgcta cgccattagc atcttcacga tgtgatttca gcgtccacgg
     6481 caggttgcgg gcaaaaccgt gcgcaggcag gccttgttgt gccgccggac caaaccacgg
     6541 ccagcaaacc ggtacgccac cgcgaatagc aacgccattt ttgaacggtg tgttgttgct
     6601 caaccacaga acttcttctt cacctgcagg tttccacgag agaaggtgtg cgccctgtaa
     6661 tgcaaaagag gcttttacct ggggatgatc gactacaatg aggtccagtt catccagttt
     6721 acgacgggag aggacagggg agatttgttc gatgaccgga agggcaaaaa ttttcttaat
     6781 catgacgcag tcctttaact tcattttatc aggtaaaaaa aagagcgacc gaagtcgctc
     6841 tttttagatc acagtgtcat ctcaacttat ttagagatgt gagcgatcag gtccagaact
     6901 ttgttggagt aaccggtttc gttgtcgtac caggatacca gtttcacgaa gttgtcgttc
     6961 agagcgatac cagctttagc atcgaacacg gaagtgcaaa cttcgccgtt gaaatcggta
     7021 gatactacgt catcttcggt gtagcccaga acgcctttca tttcgccttc agcagcagct
     7081 ttaacggcag ctttgatctg ctcgtaagtt gcagcttttt ccagacgaac ggtcaggtca
     7141 actacggata cgttcggggt cggaacgcgg aacgccatac cagtcagttt gccattcagt
     7201 tctggcagta ctttacctac agctttagca gcaccggtag aggacgggat gatgttctgg
     7261 gaagcgccgc ggccgccgcg ccagtctttg tgagacgggc catcaacggt tttctgagta
     7321 gcggtagtag cgtgaacggt ggtcatcaga ccttcgatga tgccgaagtt atcgttgata
     7381 actttagcca gcggagccag gcagttggtg gtgcaggaag cgttggaaac gatgtcctgg
     7441 ccagcatatt tgtcgaagtt agcgccttta acgaacatcg gagtgttgtc tttagacgga
     7501 ccagtcataa ccactttctt cgcaccagcg gtgatgtgtt tacgagcagt ttcgtcagtc
     7561 aggaacagac cagttgcttc agcgacaacg tcaacaccaa cttcgtccca tttcaggtta
     7621 gccggatcac gttcagcggt aacacggatt tttttaccgt taacgatcag atgaccgtct
     7681 ttcacttcaa cggtaccgtc gaaacggccg tgagtggagt catatttcag catgtatgcc
     7741 atgtaatcag cgtctaacag gtcgttgatt gcaacgatct cgatgtcaga acgtttctga
     7801 gcagcacgga aaacaatgcg accgatacgg ccaaaaccgt tgatacctac tttgatagtc
     7861 atatattcca ccagctattt gttagtgaat aaaaggttgc ctgtaaaatt acaaaaacct
     7921 tacgcagcgt caagcggaat cgtgtcaatc attgcgacaa atcaatcctg tgcctaagca
     7981 ttacgcgact gactcgcctc actcttcctt tgggcttgag accacatggg gtcggcgccc
     8041 cgaattttaa agggcaatta cgataaaaat gtgattttca tcacgattta ggtgcagcca
     8101 tttcgtcgag atcaagttta ggacaaaatt ttgccagttg gtgttaatgt tttgttagaa
     8161 tcagtcacgt aatgtgagca cgattaaagt gagatgtgag caaatggcta ataaaccttc
     8221 ggcagaagaa ctgaaaaaaa atttgtccga gatgcagttt tacgtgacgc agaatcatgg
     8281 gacagagccg ccatttacgg gtcgtttact gcataacaag cgtgacggcg tatatcactg
     8341 tttgatctgc gatgccccgc tgtttcattc ccaaaccaag tatgattccg gctgtggctg
     8401 gcccagtttc tacgaaccgg tgagtgaaga atccattcgt tatatcaaag acttgtcaca
     8461 tggaatgcag cgcatagaaa ttcgttgcgg taactgtgat gcccatctgg ggcatgtctt
     8521 ccctgacggg ccgcagccaa cgggcgaacg ttattgtgtt aactctgctt ctttacgctt
     8581 cactgatggt gaaaacggcg aagaaatcaa cggttgaaaa aacgattcag caacttattc
     8641 cacaggagcg gaaaaattat gaatcttgat gacattatca acagcatgac gcctgaggta
     8701 taccagcgtt tgtcgaccgc cgttgaactg gggaaatggc ctgatggcgt tgcgttgacc
     8761 gaggaacaaa aagagaactg cctgcaactg gtgatgctgt ggcaagcccg caataatacc
     8821 gaagcacagc atatgacaat tgacacaaat ggtcagatgg tcatgaagag caaacagcag
     8881 ttaaaagaag attttggtat ctcagcaaag cctattgcca tgtttaagta agtggttcat
     8941 cccctttttg ccgcggctat gaaggggata gccaacaatg accccacgac tcatctcaat
     9001 tatctcgcca tgacctggct aagtcccagt ccgatctctt gccgtcgcgt tcacgcctga
     9061 atgacttctc ctgaacttaa gtctcagacc tatttggccg gtaatccctc tcaaatttgc
     9121 tccttttgcg ttccgcctta catcaacctg taacagaacc agccattttc tgcgtaattg
     9181 tgatctgagt tgtaaaacgc agattattat ctgtgtttta catgtttaat gttgacctca
     9241 aaaatcatgt gggtaagtat gatgaccatc taagcaaaac aaaacacaga ggatgacgca
     9301 atgaaagcac tggctcggtt tggcaaagcc tttggcggct acaagatgat cgatgtgcca
     9361 caacccattt gtggcccgga agatgtagtg attgaaatta aagccgcggc aatctgcggc
     9421 gcagacatga agcactacaa tgtcgatagc ggttctgatg agtttaactc tatccgcggc
     9481 catgagttcg caggttgtat tgcgcaggtt ggtgaaaaag tcaaagactg gaaagtgggg
     9541 caacgcgtcg tgtcggataa cagcggtcac gtctgcggcg tttgtccggc ctgtgaacaa
     9601 ggtgattttc tgtgttgtac agaaaaagta aaccttggtc tggataacaa tacctggggc
     9661 ggtggttttt ccaaatattg tctggttcct ggtgaaattc tcaaaattca tcgtcatgcg
     9721 ttgtgggaaa tccctgatgg tgttgattat gaggacgcag ccgtacttga ccctatctgt
     9781 aatgcctaca aatccatcgc gcagcaatcg aaattcctcc ctggtcagga tgtggtcgtc
     9841 atcggcactg gcccactcgg gctgttctcc gtacaaatgg cgcgaattat gggggcggta
     9901 aatatcgtcg tcgttggtct gcaagaagat gtggcggtcc gcttcccggt tgcaaaagaa
     9961 ctgggtgcga cggcagtagt aaatggttct accgaagatg tggtggcgcg ctgccagcaa
    10021 atttgtggca aagacaatct gggactggtg attgaatgct ccggtgccaa tatcgcactg
    10081 aaacaagcca tcgaaatgct ccgtccgaac ggggaagtgg tacgcgttgg aatgggcttc
    10141 aaacctcttg atttctcgat taatgacatt accgcctgga acaaaagcat cattgggcat
    10201 atggcctatg actccacctc atggcgtaac gctatcaggc tattagccag cggcgctatc
    10261 aaagtcaaac cgatgatcac gcatcgtatc ggcctgtcgc aatggcgcga agggtttgat
    10321 gcgatggtcg ataaaaccgc aatcaaagtg atcatgactt acgactttga tgaataacca
    10381 ctctcctgaa ggaacgttat cgcatggatc agataacaaa accgcattgt ggtgcacggc
    10441 tggatcgctt accggattgc cgctggcatt catcaatgtt tgccatcgtc gcgtttggct
    10501 tgcttgtctg ctggagtaat gccgttggtg gcttgatcct cgcgcagctg aaagcgttgg
    10561 gctggacaga taattccacc acagccacat tctcagcaat cacgaccgcc ggaatgtttc
    10621 tcggtgcgct ggtgggcggc atcattggcg acaaaaccgg tcgcagaaat gcgttcatcc
    10681 tctatgaggc cattcatatt gcctcgatgg ttgtcggtgc tttctcaccg aatatggatt
    10741 tccttattgc ctgccgtttt gtgatgggag ttgggctggg agctttactg gttacgctgt
    10801 ttgctggttt caccgaatat atgcccggta gaaatcgtgg aacgtggtca agtcgggttt
    10861 cctttattgg caactggtca tatccgctct gttcattgat agcgatggga ctcacgccgc
    10921 tgattagtgc agagtggaac tggcgggtac aactgcttat ccctgcaata ttgtcgctta
    10981 tcgctacggc gctggcctgg cgctactttc ctgaatcccc gcgctggctg gaatcgcgcg
    11041 gacggtatca ggaagccgag aaagtcatgc ggagtataga agaaggcgtc atacgccata
    11101 caggtaaacc tttgccgccc gtggttattg ctgatgacgg taaagcacca caagcggtgc
    11161 catattcagc cttactgaca ggagtattac tgaaacgcgt gatattaggc tcttgtgtgc
    11221 tgattgccat gaacgttgtg cagtacacac taattaactg gttgccaacg atattcatga
    11281 cccaggggat taatttaaaa gactcgattg ttttaaatac catgagtatg tttggcacgc
    11341 catttggcat ttttattgcc atgctggtga tggataaaat tccgcgtaaa acaatgggtg
    11401 tggggctatt aatcctgatt gcggtgctcg gatatatcta ttcactgcaa accagcatgt
    11461 tgctcataac gttgattggt ttcttcctga ttactttcgt ctatatgtac gtttgctatg
    11521 cctcggcagt gtatgtccct gagatctggc cgacagaggc caaactccgt ggctccggtc
    11581 tggcgaatgc ggtagggcga atcagtggta ttgccgcacc ttatgccgtt gcagtgctgc
    11641 tcagtagtta tggcgttacg ggagtcttta ttcttctggg ggcggtttca attattgtcg
    11701 caattgctat cgccaccatt ggaatcgaaa ccaaaggtgt ctccgttgaa agtttaagta
    11761 ttgatgcagt agccaataaa taatacgggt ggattcaaaa tgaaaaattc aaaagcaata
    11821 ttgcaggtgc cgggcacaat gaaaattatt tcagcagaaa taccagtgcc taaagaagat
    11881 gaagttttga ttaaagtaga atatgtcggt atttgtggtt cagatgtaca tggttttgaa
    11941 tcaggtccgt ttattccgcc taaagaccca aatcaagaaa ttggcctggg tcatgaatgc
    12001 gccgggacgg ttgtggctgt gggaagccgt gtgcgcaaat ttaaaccggg ggatcgggta
    12061 aatatcgaac ctggcgttcc ttgcggtcac tgtcgttact gtctggaagg caaatataac
    12121 atctgcccgg acgttgattt tatggcgaca caacccaact accgcggcgc attaacgcac
    12181 tatctgtgtc atccggagag ctttacttac aaactgcccg acaatatgga cacgatggaa
    12241 gggacgctgg tggagcctgc cgcagtcggg atgcatgccg cgatgctggc agatgttaaa
    12301 ccgggtaaga agataattat tctgggagca ggttgtattg gtttgatgac gttgcaagcg
    12361 tgcaaatgcc tgggagcaac ggaaattgcc gtcgttgatg tgctggaaaa acgtctggca
    12421 atggcggaac agcttggtgc gacagtggtt attaacggcg caaaagaaga cactattgca
    12481 cgctgtcagc aatttaccga agacatgggc gcagatattg ttttcgaaac agcgggttct
    12541 gcggtcaccg ttaaacaggc accttatctg gtaatgcgtg gcggtaaaat tatgattgtt
    12601 ggtactgtac ccggcgcttc ggcaatcaat ttcctcaaaa tcaatcgcga agtcactatc
    12661 cagacggtat tccgctatgc caatcgttat ccggtcacga ttgaagcaat ttcttcaggg
    12721 cgattcgatg tgaaatcgat ggtgacgcat atttacgatt atcgggatgt acaacaggca
    12781 tttgaagagt cagttaacaa caaacgcgac attattaaag gcgttattaa gattagcgat
    12841 taattaatac gggagaacag aacatgctcg cagatatcag atattgggaa aacgatgcca
    12901 caaataaacg ttatgctatt gcccatttta atgtatggaa tgcagaaatg ttgatgggcg
    12961 ttatcgacgc agccgaagaa gcgaaatcac cggttattat ttcttttggc acaggttttg
    13021 tcggcaacac ctcatttgaa gatttctctc acatgatggt atctatggca caaaaagcaa
    13081 cggtgccggt aataactcat tgggatcatg gtcggagtat ggagattatt cataacgcct
    13141 ggactcatgg tatgaattca ttaatgcgtg atgcttccgc atttgatttc gaagaaaata
    13201 ttcgtttaac caaagaggct gttgatttct tccatccgct gggtattccg gtagaggcgg
    13261 aattagggca tgtcggtaat gaaaccgttt atgaggaagc gctggcgggc tatcactata
    13321 ccgatcctga ccaggcggct gaatttgttg aacgtacggg ctgtgactca ttggctgtcg
    13381 ccatcggcaa ccagcatggg gtttatacgt cagagccaca attgaacttt gaggtcgtca
    13441 aacgcgtacg cgatgcagtg tctgttccgc tggttttgca tggcgcatcg gggatcagtg
    13501 atgccgacat taaaactgca atttcgttgg gtatcgcgaa aatcaacatc catacggagc
    13561 tctgtcaggc cgcaatggtc gcagtgaaag aaaaccagga tcagcctttc ctgcatttag
    13621 aacgtgaagt acgtaaagct gtaaaagaac gggcattgga aaaaattaaa ctgttcggtt
    13681 ctgatggcaa agcggaataa tgatatggat aatctcgacg ttatttgtat aggtgccgct
    13741 attgttgata ttccattgca accggtcagt aaaaatatct ttgatgtgga ttcttatcct
    13801 cttgaaagaa tcgcaatgac caccgggggt gatgcaataa atgaagcaac aattatttct
    13861 cgtctgggcc atcgtacagc attaatgagc cgtattggta aagatgccgc agggcagttt
    13921 attctcgacc attgccgcaa agagaatatt gatattcaaa gcctgaagca ggacgtcaac
    13981 atagatacct ctattaatgt tggactggtg acggaggatg gcgagcggac gtttgtcaca
    14041 aaccgtaacg gcagtctgtg gaaattgaat attgacgatg ttgattttgc gcggttttcc
    14101 caggcgaaat tattatcact ggccagtatt ttcaacagtc cactattgga tggtaaagcg
    14161 ctaacagaaa tttttacgca agctaaagcc cggcagatga atatctgtgc cgatatgatt
    14221 aaaccgcggt tgaatgaaac gctggatgat atttgcgaag cattgagtta tgtcaattat
    14281 gccgaggcaa aattactcac cgggaaagag acactggatg aaattgctga cagctttctt
    14341 gcgtgcggcg taaaaacggt ggtgattaaa acgggtaaag acggctgctt tatcaagcgt
    14401 ggtgacatga cgatgaaggt gccggcggtc gcaggaataa ccgccatcga caccattggc
    14461 gcgggcgata actttgcttc aggttttatt gcggcactgt tagaaggaaa aaatctgcgt
    14521 gaatgcgcac gctttgccaa tgcaacggcg gctatctcgg ttctaagcgt cggtgccacc
    14581 accggcgtaa aaaacagaaa gctggtcgaa caattgctgg aagaatacga aggataatga
    14641 aggcaaatga aaaagatacc tttaggcaca acggatatta cgctttcgcg aatggggttg
    14701 gggacatggg ccattggcgg cggtcctgca tggaatggcg atctcgatcg gcaaatatgt
    14761 attgatacga ttcttgaagc ccatcgttgc ggcattaacc tgattgatac tgcaccagga
    14821 tataactttg gcaatagtga agttatcgtc ggtcaggcgt taaaaaaact gccccgtgaa
    14881 caggttgtag tagaaaccaa atgcggcatt gtctgggaac gaaaaggaag tttattcaac
    14941 aaagttggcg atcggcagtt gtataaaaac ctttccccgg aatctatccg cgaagaggta
    15001 gaagccagct tgcaacgtct gggttttgat tacatcgata tctacatgac gcactggcag
    15061 tcggtgccgc cattttatac gccgatcgct gaaactgtcg cagtgcttaa tgagttaaag
    15121 gctgaaggga aaattcgcgc tataggcgct gctaacgtcg atgctgacca tatccgcgag
    15181 tatctgcaat atggtgaact ggatattatt caggcgaaat acagtatcct cgaccgggca
    15241 atggaaaacg aactgctgcc gctatgtcgt gataatggca ttgtggttca ggtttattcc
    15301 ccgctagagc agggattgtt gaccggcacc atcactcgtg attacgttcc gggcggcgct
    15361 cgggcaaata aagtctggtt ccagcgtgaa aacatgctga aagtgattga tatgcttgaa
    15421 cagtggcagc cactttgtgc tcgttatcag tgcacaattc ccactctggc actggcgtgg
    15481 atattaaaac agagtgattt aatctccatt cttagtgggg ctactgcacc ggaacaggta
    15541 cgcgaaaatg tcgcggcact gaatatcaac ttatcggatg cagacgcaac attgatgagg
    15601 gaaatggcag aggtcttggg gcgttaaata ctcaacacga ttcacctctg tttctgtgaa
    15661 aactgtgatg aaaatctgtg attcaaacag gttattttga aagtaaacat cggttatgcg
    15721 ataatcgcgc taatgtgatg taaaaagata caggggtgta atcgtggcgg caaaagacag
    15781 gattcaggca attaagcaaa tggttgccaa cgataaaaag gtgacagtct caaatttgag
    15841 tgggattttt caggtaaccg aagaaaccat tcgccgcgat cttgagaagc tggaagatga
    15901 aggctttttg accagaacct atggtggtgc tgttttaaat acagcgatgt tgacggagaa
    15961 tatccatttt tataagcgcg cttcatcgtt ttatgaagag aagcagctta ttgcacgcaa
    16021 ggcactaccc tttatcgaca ataaaaccac catggcagcc gattccagta gtaccgtaat
    16081 ggaattgctc aaattgttac aggaccgtag tgacctgacg ttgctaacca actcggcgga
    16141 agcgattcat gttctggctc agtcagaaat taaagtcgtt tcaacgggtg gggaactaaa
    16201 caaaaataca ctttcactgc aaggaagaat tactaaagag atcatcagcc gctatcatgt
    16261 cgatatcatg gtaatgagtt gtaaaggtct tgatattaac agcggcgcgc tggactctaa
    16321 tgaagcggaa gctgaaatca aaaagacaat gatccgtcag gcgacagaag ttgcgttatt
    16381 ggttgatcac tctaagtttg atcgcaaagc ttttgtccag ttagccgact ttagtcatat
    16441 taattacata ataactgata aatcaccggg tgcagagtgg atagcatttt gcaaagacaa
    16501 taatatccag ctggtgtggt aatttaagca cgcgttgtca atattatttg ttcctgcgcc
    16561 tggaaataaa atgatgataa agatgcggca agtgaatttg ccgcaattgc tacctttttt
    16621 agcaaatccg aggcacccaa tggaacaata tgatcaaatt ggcgcaagac tggaccgctt
    16681 gcctttggcc cggtttcatt atcgtatatt tggtattata agctttagtc tgttattaac
    16741 a
//
LOCUS       DAZFAB010000096        16714 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZFAB010000096 DAZFAB010000000
VERSION     DAZFAB010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16714
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.096"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..861
                     /locus_tag="ACHJWA_003631"
     CDS             <1..861
                     /locus_tag="ACHJWA_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4310151.1"
                     /translation="GFFETGRGIVDTVVAFSALAVFTWFGSGLLGFKAGIWFYSLIVI
                     AVGITIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLKDKTIWLIAFNVFFVYAVYCG
                     LTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGGMISDKILKSPSKYLCYTFI
                     ISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAVFFAPIGEAKIAENKTGAAM
                     ALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMACFAFCGAVVSVMLVKRISQ
                     RKKEMLAAEA"
     gene            complement(952..1644)
                     /gene="ompL"
                     /locus_tag="ACHJWA_003632"
     CDS             complement(952..1644)
                     /gene="ompL"
                     /locus_tag="ACHJWA_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HFO4310152.1"
                     /translation="MKNINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSIGS
                     GGAVYLDVNYKFTPWFNLTVRNRFNHNNYSSTDLNGDLDNNDSYEIGNYWNFIITDKF
                     SYTFEPHYFYNINDFNSGNGTKHHWEITNTFRYRINEHWLPYIELRWLDRNVEPYHRE
                     QNQIRIGTKYFF"
     gene            complement(1711..3114)
                     /gene="yihO"
                     /locus_tag="ACHJWA_003633"
     CDS             complement(1711..3114)
                     /gene="yihO"
                     /locus_tag="ACHJWA_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4310153.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLSIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFSLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPLTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCI
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEEKTASVTL"
     gene            complement(3157..4542)
                     /gene="yihP"
                     /locus_tag="ACHJWA_003634"
     CDS             complement(3157..4542)
                     /gene="yihP"
                     /locus_tag="ACHJWA_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4310154.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFIGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQQLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPANPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFSGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            complement(4588..6624)
                     /gene="yihQ"
                     /locus_tag="ACHJWA_003635"
     CDS             complement(4588..6624)
                     /gene="yihQ"
                     /locus_tag="ACHJWA_003635"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HFO4310155.1"
                     /translation="MDTPRPQLPDFEFHQNNDSFTLHFRQRLILTHSKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNKEGVQFLAYI
                     NPYVASDKDLCEEAAKRGYLAKDVAGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGISAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAQTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGETFHGGEITVEAPIGKPPVFYRADSEWAALFASLKNI"
     gene            complement(6732..7607)
                     /locus_tag="ACHJWA_003636"
     CDS             complement(6732..7607)
                     /locus_tag="ACHJWA_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFO4310156.1"
                     /translation="MDFFTLFSPDGVMLETNVVCIVIALMFLYTFVGICAGFGGGLTT
                     MPLVTLMLPVKMATPLSVIVGTATALYATWLSRKETDWRSAMVLIIFSFLGIPVGLYA
                     LSYLPDHFMKVGLGGFLILYSFYSMFIPRLPIYDRSWIAAPIGAVAGALGAAFSTNGP
                     PMVIYGMLRNLGPAAFRGTLNAFFTANNIAVIGGLATSGILTVSIFKLVLFCIPTMIL
                     GSLVGQYVHKRISVKVFRVMVFLLLIASGSMLIKGALGIPAAAALLPPFTLLVILQLV
                     FGKKVAAIRQAEASK"
     gene            complement(7668..8570)
                     /gene="yihR"
                     /locus_tag="ACHJWA_003637"
     CDS             complement(7668..8570)
                     /gene="yihR"
                     /locus_tag="ACHJWA_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HFO4310157.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHGSKAAIHGLLAWRDWQI
                     SELTATSVTLTAFLPPSYGYPFMLASQVVYSLNARTGLSVEIASQNIGTVTAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHANPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEITITHPQQALSVSLCSDQPWVQIYSGEKLQRQGLAVEPMSCPPNAFNS
                     GLDLLLLEPGKTHRLFFNIYGQHN"
     gene            complement(8637..9878)
                     /gene="yihS"
                     /locus_tag="ACHJWA_003638"
     CDS             complement(8637..9878)
                     /gene="yihS"
                     /locus_tag="ACHJWA_003638"
                     /EC_number="5.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HFO4310158.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWDYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPR
                     IPLAPGLAPAVAAGLLDINAK"
     gene            complement(9894..10772)
                     /gene="yihT"
                     /locus_tag="ACHJWA_003639"
     CDS             complement(9894..10772)
                     /gene="yihT"
                     /locus_tag="ACHJWA_003639"
                     /EC_number="4.1.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HFO4310159.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKILSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLSASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            complement(10796..11692)
                     /gene="yihU"
                     /locus_tag="ACHJWA_003640"
     CDS             complement(10796..11692)
                     /gene="yihU"
                     /locus_tag="ACHJWA_003640"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFO4310160.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRSVLFGENGVCESLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFNMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            11860..12756
                     /gene="yihV"
                     /locus_tag="ACHJWA_003641"
     CDS             11860..12756
                     /gene="yihV"
                     /locus_tag="ACHJWA_003641"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HFO4310161.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYKQAKSSQSAIMVDAK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVVLATGGDLAESVRFASGVAASKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            12790..13575
                     /gene="csqR"
                     /locus_tag="ACHJWA_003642"
     CDS             12790..13575
                     /gene="csqR"
                     /locus_tag="ACHJWA_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO4310162.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            13674..14273
                     /gene="yihX"
                     /locus_tag="ACHJWA_003643"
     CDS             13674..14273
                     /gene="yihX"
                     /locus_tag="ACHJWA_003643"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFO4310163.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGTWSDLTRIPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            14267..15139
                     /gene="yihY"
                     /locus_tag="ACHJWA_003644"
     CDS             14267..15139
                     /gene="yihY"
                     /locus_tag="ACHJWA_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFO4310164.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            15136..15573
                     /gene="dtd"
                     /locus_tag="ACHJWA_003645"
     CDS             15136..15573
                     /gene="dtd"
                     /locus_tag="ACHJWA_003645"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFO4310165.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            15618..16559
                     /gene="fabY"
                     /locus_tag="ACHJWA_003646"
                     /gene_synonym="yiiD"
     CDS             15618..16559
                     /gene="fabY"
                     /locus_tag="ACHJWA_003646"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFO4310166.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
BASE COUNT         4154 a         4139 c         4311 g         4110 t
ORIGIN      
        1 ggcttcttcg aaacagggcg tggcattgtc gataccgtgg tggcattttc tgcgttggca
       61 gtatttacct ggtttggcag tggcttatta ggttttaaag caggcatctg gttctattcc
      121 cttattgtga ttgccgtagg cattactatt ttctttgtcc tgaatgacaa agaagaggca
      181 ccgtccgttg aggtgaaaaa agaagacggg gcatcgcaaa acaccagtat gacctcggtg
      241 ctgaaagaca aaactatctg gcttatcgct ttcaacgtct tcttcgttta cgcggtttac
      301 tgtggcctga cattcttcat tccattcctg aaaaacatct atctattgcc cgttgcgctg
      361 gtgggggctt acggcatcat taaccaatac tgtctgaaaa tgattggtgg accgattggt
      421 ggcatgattt cagataaaat cctgaaatcg ccgagtaaat atctatgcta cacctttatc
      481 atcagtaccg ctgcgctcgt actgttgatt atgctgccgc acgaaagtat gccggtctat
      541 ttagggatgg catgtacgct gggctttggc gcgatagtct ttacacagcg agccgtattt
      601 tttgcaccta tcggcgaagc aaaaattgct gaaaataaaa caggcgcggc gatggcgttg
      661 ggtagcttta ttggttacgc cccggcgatg ttctgcttca gtctgtatgg ctacattctg
      721 gatttaaatc cggggattat tggctacaaa atcgtgtttg gcattatggc ctgcttcgca
      781 ttctgtggtg cggtggtttc cgtaatgctg gttaagcgta ttagccaacg taagaaagag
      841 atgctggcgg ctgaagctta attaatggcc ggatgtactt aatccggcca acgaaaaccg
      901 tatcaaccgt aggccggata aggcgtttac gccgcatccg gcagtttcac atcagaagaa
      961 atacttcgtc ccgatacgaa tctggttttg ctcgcggtgg tacggttcca cgttgcgatc
     1021 taaccaacgc aattcaatat agggcagcca gtgctcgtta atgcggtaac ggaaggtgtt
     1081 agtgatttcc cagtgatgct tcgtgccatt gccgctattg aaatcattaa tgttataaaa
     1141 ataatgcggc tcaaaggtat atgagaactt atcggtaata ataaagttcc agtagttgcc
     1201 aatctcgtag ctgtcattat tatccagatc accgtttaaa tcggttgagc tgtagttatt
     1261 atggttaaaa cggttgcgca ctgtcaggtt aaaccacgga gtaaatttgt agttaacatc
     1321 cagataaaca gcaccacccg aaccgatact cttatcatta attaagccac cgggctggat
     1381 ggttaattta tcggttggtt taaataacgg ataccaacct tcgatttcgt tatatccatg
     1441 ttttagttca tcttctcgct gaaaggtgta ggtattggtt aacataatac ccgcgcccat
     1501 atcgaagtta taacccacac gtagcataac ctcgccctga tcggaggcga gattatatgc
     1561 ctccctgttt tcgacgtatg cgccagcgaa tactgaggca gaggtcagag aagataataa
     1621 aattattgca ttaatatttt tcataataag tccattttta aacgtaggga ggccgccacc
     1681 caccaggatg gaaatagcgg ctattgacat ttataacgtt acggaagccg ttttttcttc
     1741 tgtcgccatc tctttatttt tgcgttggtt gatttcctca ataatcaacg caaaccgttt
     1801 ttcattgagt gtgtaaaaga atcccatcgt caatgcagca ataattgcca gtgcacaagg
     1861 ccagataaaa atgagctgac gcaaaccctg taatgtcgca tcactttgcg cgatgttggg
     1921 aacataaccg atttgcgtca gcataatgcc tggcaagaag ccagcaagtg cggcagaaat
     1981 tttacgaaag aaggtataac cggtataaac agaaccttcg gcacgaatac cggttttcca
     2041 ttcaccgtaa tctacggtat ctggcaccag tgcccagttc aggctgttta caaaggccgt
     2101 gccaaaaaag gcgatacaag agaacatgac gaaagtgaaa gagttacttc cccagaaata
     2161 attcaggata tcaccaaccg cccatagcac catgccagct aaataaacct gttttttacc
     2221 aaaacatttt acagttaatg gcaccagtaa tacgccgacg aggatgcatc ccatactgaa
     2281 gaatcccatc cacgacaata aattaatatc attcagcaca tactgggtgt aatagacctg
     2341 aatcgccagt ttgatattaa atgccgccag ggtacacaag ttagcaatgc ataaaaccag
     2401 caatggcgga ttccgaaata tcgcgcaaaa agatttgaga atactggctt tatgtccggt
     2461 tggtgctgtg tccacataat gctctttgac gcctctgtag cacagcatca taaaaataaa
     2521 gccaccaatg gagaacatca gtgccgcaca ggcataaccg acggttgagt cagaaaaaag
     2581 cgactgcaac ggaataaacg ccacggtaca aatcaatagc cctatggttg caccaccctg
     2641 gcggtaagcc gcgagctgcg cgcgctcatt cgggttttta gtaatggccg ggatcatcgc
     2701 accatacgag cagttcatca gactgtatga aagtccaaac atcataaaca gcgcggtggc
     2761 aatcgtcgtt ttaaccggca aactaaaagt ggtggcgata aactggagcg ttgcaattaa
     2821 cgccgccgga accgctgcgt ataaaataaa gggacgaaat ttgcctttag gcccaatatt
     2881 tttccgcgaa tcgagtaaaa atccggtgag catatcggta aacgcggtaa aaaacttcgc
     2941 caccagaaag ataatcccgc catagtaagc tggcatccct aattcatcgg tataaaattt
     3001 gaggagatac agagtaccga tgctaagcat taaattcgaa ccaacgtcgc ccataccata
     3061 ggcgattttt tcccgcagat tcagttttaa tgtcagtgga ttatggtcag acataatcac
     3121 tccttataac gccccgcagg gcattgttta ttattattac gccgtgcgtt tacgggcttc
     3181 tatttcttca acaatgcgga catacatctt ctcgttcagg ctgtagaagc agcccatcgc
     3241 cacaatggtg actaccgcca gtgcgcttgg gtagatgaag atcagctggc gcaacccttc
     3301 aatggtgtgg tcagcctgcg ccacgttcgg cacataaccg atttgcgtca gcatccagcc
     3361 ggggaagaaa ccagccagcg cctgagacac tttgcgaaag aaggtgaagc cggtgtagac
     3421 cgttccttcc gaacgaacgc cggtacgcca ctcgccgtac tcgacggtgt cggaaaccag
     3481 cgcccagttc aggctgttaa caaacgctga gccgaagaat gccaggcagg agaacgccac
     3541 gaagctgacc gaaccgccgc tgaagaaata gttgagcaga tcgcccagca cccaaatcag
     3601 taggccgccg atatagacct tcttcttacc aaaacgcctg actgcgccag gcatcaggaa
     3661 cacaccgatg aaaatacagc ccatgctgaa aaatcccata tatgacaaca ggatgggatc
     3721 gttaagtacg tactgggtgt aatagacctg gatggcgagc ttgacgttaa acgcccctaa
     3781 agtgcagagg ttggcaatgc acagaatgaa cagtgggcgg ttaccggcaa ttgcgcggaa
     3841 agattgcagc aggccaggct tttgcgccgg attggctggt tgggtttcga cataacgctc
     3901 tttcacgccc gagtagcaga tccacataaa cagcaggccg aacagtgaaa acagcgtggc
     3961 ggcgaagata tagccaagtt gctgattacc ttcgataaga ttcataaccg gcacgaatcc
     4021 caccgtgcac agcagcaggc ccagcgtagc gcctccctga cgccatgccg ccagtgatgc
     4081 gcgctcgttg gggtttttgg taatagcagg caccatcgcg ccataggagc agttcatcat
     4141 gctgaaaaac agcccgtaga gcataaacag aatagtggcc atcaccgttt taccagtgac
     4201 atcgaacggt gtgccgataa agttagcgat tgccaataag gtgaccggga atgacgcgta
     4261 caaaatgaaa gggcggaact tacctttcgg gccaatctta cgccgggaat ccaacatgat
     4321 gccggttccc atatcggtaa aggcggtaaa gaatttcgag atcaaaaaga taatgccgcc
     4381 ataggtgcca ggtagcccca gaacgtcggt ataaaacttc aaaagataaa gcgtaccaat
     4441 atccagcagg atgttagagg ccagatcgcc gataccgtaa gagagtttct ctttaaaggg
     4501 caggcgcaaa gttgccggat cttccgttgt gatgtgactc atcgttattc tccatttgcc
     4561 cgtgagtttc cccacgggcg ggcatgatta gatgtttttt aacgaggcga acagtgcagc
     4621 ccattcgctg tcggcgcgat aaaagaccgg cggcttgccg atgggcgctt caacagttat
     4681 ttctccaccg tggaaggttt ctccggtcca ggcgtggacc cagttatcct ctggcagata
     4741 gagcgtccag tcgctgcgac cttcttcgtg taccggagca accagaatgt cgcgacctaa
     4801 caggtactga tatttcaggg aataggtctg cgcgtcgtct tcgtaatgca ggaacagcgg
     4861 gcgcataacc ggcaggccgg acttcgcatt cagcgcgacg gcctctttca ggtaaggttt
     4921 cagggtggtg aagacggtgg tcatacgggc gaaatgggcg atggtttccg cgtcgccgtc
     4981 aaactgccag ttgtcgccag gacggttacc ttcgtgggtg cgcatcatcg gcgtgaaggc
     5041 gctgaaatcg caccagcgca gcagcagctc tttgctgcgc ttcatctcaa acagggtggt
     5101 ataaccgcca atgtcgctgt gatgcaggcc atgtccggtc attgccagag atagcgccgc
     5161 cgggacaacc gacgccaggc catcgtccag actccagtcc acgttctggt cacccgccca
     5221 catcatggtg gagtatttct ggctaccggt agaaccggcg cgcataaaga agaggatctc
     5281 gccgagcttg cccgtttctt caagggcttc gtagttacat ttcgcccaca gcgcgggcca
     5341 ggcgttatgc ataatttccg cgctgatgcc gttatgcagg tacgtgtcgg tgggcagata
     5401 ctcgccgaag tcagccatcc agccgccgca gccgagttca atcatgttct ttttgataac
     5461 ttccttgaac caggcgtagg cttccggatt ggtgagatcg acaacgccgc cgtagaactc
     5521 gccaaactcc accagatagt cgccaccggc gacatctttt gccagataac cgcgctttgc
     5581 cgcctcttcg cagagatctt tatcactggc aacatacggg ttgatatagg caaggaactg
     5641 cacgccctct ttgttccact gcttgatacg cgaatccagt tgcgggtagt tttcgctgtt
     5701 ccacttccag ttccacatca cgcgtttgcc aaaagaggtc atacgaatac cggaccagtc
     5761 ctgcgcccag atgccgttga ccttcacgcc cgcgttacgc atggtgtcca gtttcttctg
     5821 gcacacttcc gtcccgccct gaatgccgag cgttactccg tcataaatcc agtcgggcag
     5881 ttctggctgg cgtcccagca gggcggttaa tttttccagc agggagatgt atgtgtcagc
     5941 acattcaaaa cgcagcgttg ctttgtcttc ccacagcgcc agttcatggt attccggggc
     6001 actaaagtcg aagttcatat agcaactgtt atcaacatgg caataatact tctgcgtgct
     6061 gacaaacgta ggctgtggga agaaagtcca gtaatagtcg ccgcccgcat tttctttgca
     6121 gtcggcctgc caggtgacat aggtttgttt gttgcgacca acgccttgtt cactggtcca
     6181 tagcgggaac ggtttgccgc gcagatcgaa gtaggaaaac tgttcgccgc agccgtagat
     6241 atggtcctct ggttgagcgg caaggcgcag ccagatgcgg ttgtggttaa ggttgtcgtt
     6301 ttgtagttcc agcaataaac ggccctgatc gtcggcagag atattcagcg tggcgctaat
     6361 gtcagaacca cgactgaaat ggattaacca accatccggt gactggctga cgatggcgtc
     6421 ggtaagcgca attttctcct gtagtttatc tttaatgctg aaattgccgc ggaacatatc
     6481 gatgtccgct atacctgagc caatccataa acaaggatta tctttgctat gggttaaaat
     6541 aagacgttgt cgaaaatgta gggtaaaact gtcgttattc tgatgaaatt caaaatctgg
     6601 taattgtggg cgtggcgtat ccataaataa tctccataga gtataaagct aatatttcat
     6661 gccatcctgt tattacaggt ttaattaatt aaagaatgtg tgcggatata aatttaaacc
     6721 cgcataagtg attatttact tgcttcagcc tgacggattg ccgccacttt tttaccaaat
     6781 accagttgca gtataactaa caaggtaaac ggaggtagaa gtgcggcagc ggcgggaatt
     6841 cccagcgcgc cttttatcaa catggaaccg gaggcaatca gcaataaaaa gaccatcaca
     6901 cggaacactt tgactgaaat acgtttatgc acatattgtc caaccagtga accgaggatc
     6961 atggtgggaa tacaaaacag caccagttta aagatcgaaa cagtcaaaat tccgctggtc
     7021 gccagcccgc cgatcaccgc gatgttgttt gcggtgaaga aagcgttcag agtgccacga
     7081 aatgccgccg ggcctaagtt acgcagcata ccgtagataa ccatcggtgg tccgttggtt
     7141 gagaaagctg cgcctaatgc gcctgctact gcaccgatag gtgcggcgat ccagctacgg
     7201 tcataaatcg gcaggcgtgg aataaacata ctgtaaaagg agtacaggat aaggaatccg
     7261 cctaagccca ctttcataaa atggtcgggt aaatacgaca gtgcatataa accgacgggt
     7321 attcccagaa acgagaaaat aatcagtacc attgccgatc gccaatccgt ttctttacgt
     7381 gacagccagg tggcgtacaa ggcggtcgcg gtgccgacaa ttaccgagag tggtgttgcc
     7441 atttttacgg ggagcattaa cgtgacgagc ggcatagtgg ttaaaccacc gccaaatccc
     7501 gcgcatatcc ccacaaaggt ataaagaaac attaacgcga taacgataca taccacgttt
     7561 gtttccagca tgaccccatc cggtgaaaat aacgtaaaaa agtccataat atttcctgtt
     7621 gtgtaaatac aactctaaac aatgatctcg ttcttttctt aataaaatta attgtgttgc
     7681 ccgtaaatat taaaaaacag gcggtgcgtt ttccctggtt ccagtaataa taagtcgaga
     7741 cctgaattaa aggcgttagg cgggcaactc ataggttcaa ctgccagtcc ctgacgctgt
     7801 aatttttcac cgctataaat ttgtacccag ggttgatcgg aacaaaggct aaccgaaagc
     7861 gcctgttgtg gatgggtaat ggttatttcc cacagatcat tagcggtttt aaaggtgtgg
     7921 tcgatttttg tcgcggctat ttttttcgcc tgcgtgaaat ttaaatccag ctcatcaacg
     7981 tggtgaagcg ttgtcggatt agcgtgctca tcgacggcat aaacctggtt tgccggaagc
     8041 tggaacagat attcatcaac agatgttaag ttgcaggtta aataaggatg aatacccaca
     8101 ccatagggtg cggtcacagt accaatattc tgacttgcga tctcaacgga aagacccgtg
     8161 cgtgcattga gcgaataaac aacctgcgaa gccagcataa acggatagcc gtaactgggc
     8221 ggcagaaaag ctgtcagggt aacgctggtg gcggttaatt cgctgatttg ccagtcacgc
     8281 caggcgagca gcccatgaat agcggctttt gaaccatgtt cattaatggg taactgatat
     8341 tcctgcccct gatagcgata acagccgttg gcgatccggt tcggccacgg aataaggact
     8401 ttgcccagat gtgccagcgg catctcttcg ggtttatgtg gaataactaa atgacagccc
     8461 tgaaaggtta attcagccag acctgcaccg acggtcacaa tggtcgcgtg ataatccccc
     8521 gcggcaagtg aaacggtctg tccactgcaa tgcatgttgg tgatttgcat acgaggtacc
     8581 tttattgaat aacgacatac ccggcgtgca gcctgatatg tcgttttttt agtcagttat
     8641 ttcgcgttaa tatccagcag acccgccgca actgccggag ccaggcctgg cgctaacggg
     8701 atacgcggga tcaccaggca atgcagcagg tgataaatat cctgtttgcc gtcccagact
     8761 ttggtggtga ccttattgtc cgcatccagc tcctgccacc atgaaccatt ttcgtagtcc
     8821 atcaggtatt tgatgcagta gtcccaccat gtctgatacc aggtttcgta ctggcgatcg
     8881 ccggtgacgg tgtagagcgc gtaggccgta cccatcgctt cgacgatagg ccaacgcaca
     8941 cgttcgcgga ccaccggttt tccttcccag tcaacggtat aaacaatccc gtccgcacca
     9001 tcgggtgccc aggcatcacg cacggtggcg ttaaacagac ctttggcatc ttctagcagc
     9061 catgctggcg gttgttcgca acgggcttcc agggccgcgt ggatgtgcag cattaaacgg
     9121 ccccattcga tccagtggcc tggtgtacct ccgaacgcgc ggaagcggtg cgccgggtta
     9181 tctttgttgt aatccggcag cggattccac tgggtatcga agtgttcgtt aacgcgataa
     9241 tgattatttc ttgcgacgtc gtggataatc acggaagcca cgcgaatcgc gcgatccagc
     9301 cattttttgt catgcgttac gtcataaaca attaagaaag cttccactgc gtgcatattg
     9361 gcattgccgc cgcggtactc ttcggttttg ctgaaggctt cgtcccagga ctccaggcac
     9421 atctgctctt cttcgctcca gaagtatttc tcgataattt caatggtgta atcgagcagc
     9481 ttgcgcgctt ccgggtgacc cgttgtgacg gcgctggcag cacccagcag agcaaagaaa
     9541 tgttgatagc cctgtttgga ggcatccacc acgccctcgt cattcacaca ggcataccag
     9601 ccgccatatt ttttatcgcg cagtgcgccg ttcatggctt taataccgtg atcaaccaac
     9661 gagtaagcgc caggtcgacc catcgccgca gcaacggaat aaacgtgcaa catacgagct
     9721 gtgatccaca gatgggtgcc catctcttct ttgatttgcc ctttattgcc taaccagcca
     9781 aatccggtcg gtactacgga atttttacca aaatcaaaga tgcgatcggt ttcctgttcc
     9841 agccaacggt tgtgacttaa agtgttaaac catttcattt ttgtatcccc attttaacgg
     9901 cgtttagcca tcatttcgtc gacgatatcg ccaagttgtt gtaatttcgg tgcgcaaacg
     9961 tcacgcagca tcagctcgtt gtctggtaaa ccgacgaccg atgcccagac agcacgacca
    10021 gccaggaagc ccgatgcacc cgctgtcatt gccacgcgta cggcacgcgg gaacagtttt
    10081 tcgtcgacac cggaagagag gatcacccac ggcatattga tatggtcatt cagacgttgt
    10141 gaagcactga gaagctcttg ttgcggacct ttaccgtaaa gcggcatttc aactttgtag
    10201 aggtcagcgc cactgtcgcc cagctctttg gcggcatcga tgatcgcttg ttcgcgatcg
    10261 aatttatcgc cacgacgtgg tgggcggacg actggctcaa taatgcttac caggccgtgt
    10321 gagtggcaca gttcgttaaa ctctttcacc atatccaggc gttgctgcgc atcttcatca
    10381 ctacgccaca gcaccagcag ttttaaggct ttgccaccgt cttgtttgat ctgtagcgga
    10441 ttgattttgc ggtcaatgac cacgctatct accggaatac cgttgccagg aatgaactca
    10501 tcggcggcga caatcatggc gcaacttttg gcaatcgcgt tttgctcaac cacctggcgg
    10561 taacagaatt gttgatctac cagaatcgcc gaggcataag gcgagaggat cttcgccgcg
    10621 ttaactttga aatcagttaa aacgctatcg gctaccggag cgggtgcccc agccgcggca
    10681 aacatcatgc gcatggcttc gcgctgatcg accgccagca tggcaaaacc gcccgatgcg
    10741 cgcgtaatgt cgttgatggt gtacttattc attcatttat tcctttatcc agtcgttaca
    10801 tttttacttt ggcagtcatc ccggcactga cacggacctg ttccagaatg gcggaccagt
    10861 cctggcgacc gcgacccgct gcgcgcgcct ggctataaac ctcccgtgag gcggccccca
    10921 gcggcattgg cacatgcagc tggttggcga catcaagggc gatgccaaga tccttatggg
    10981 caagatcgat catgaaggcg ggagaaagat ccccgctgag gactttgttc ggccaggaag
    11041 tggtgaagtg gcctttaccg gcggcggtac cgctcatcac tttgacggca acatcgaagg
    11101 gaagattcag ggcttcgcac aaaacggcag cttctgccga aagcgcattg agcgcgatgc
    11161 tcatatagtt gttgatgagc ttaacgcgga tccccatgcc cggaccgcct gcgttgatca
    11221 actcactgcc catcgccatc aggatcggcg tggcacgttc aacttgttca gcggtgccgc
    11281 cagccagcag taacagagta ccggtaatgg catttgcaga agtacggcct accggaacat
    11341 ccatcatgtt gaagcctttg gcttgcatat cggcaatcaa tttatcggtt tgcagcggat
    11401 ggatggtcga catatcaatg accagcgcat cggtagataa gctttcgcaa acgccgtttt
    11461 caccgaacaa cacgctgcgc accagatcgc cattcggcaa catggtaatg ataaattcgg
    11521 catctttagc ggcctgcgcc gggttggcgg cgggagtcgc gcctttgtct accagatgcc
    11581 gcacagcctc ggcattcaca tcaaagacgc gaagttggtg cccttgctgc aataaattgc
    11641 tcgccattgg cgaacccatt tgtcctaaac cgataaacgc gattgctgcc ataaccttct
    11701 cctgaataca gtgatgtcac tttttgtcat ttatgacacg ctttgattgt ctgtttttga
    11761 tcgtatttgt aatttatagt caaaaaattg acagccgtca ctttttaaac aattggtgaa
    11821 attaaaatga acgcatccca aaatgtttaa ggaatgacca tgattcgtgt tgcttgtgta
    11881 ggtataaccg tgatggatcg catctattac gtggaagggt taccgacgga gagcggtaaa
    11941 tacgtggcga gaaattatac ggaagttggt ggcgggccag cggcgacggc agcggttgcg
    12001 gcggcaagac tgggagcgca ggttgatttt attgggcgcg tcggtgatga cgacaccggc
    12061 aacagcctgc tggcagagct ggaatcctgg ggcgttaaca cccgctacac caaacggtat
    12121 aaacaggcga aatcgtcgca atccgccatc atggtggatg ccaaaggcga gcggataatc
    12181 attaactacc ccagcccgga cctgctgcct gacgcagagt ggttggagga aattgatttc
    12241 tctcagtggg atgttgtgct ggcagatgta cgctggcacg acggcgctaa aaaagccttc
    12301 actctggccc ggcaggcggg cgtgatgacc gttctggacg gtgatattac gccgcaggat
    12361 atcagcgagc tggtggcatt aagcgatcat gcggcctttt cagaaccggg tctggcgcgc
    12421 ttaacgggcg tgaaagagat ggccagtgcg ctaaaacagg cacaaacgct cacaaatggt
    12481 catgtctatg tgacccaggg cagcgcaggc tgtgactggc tggaaaatgg cgggcgtcaa
    12541 catcaaccgg ccttcaaagt tgatgtggta gataccacag gtgcgggtga tgtttttcac
    12601 ggcgcattgg cggtggtgct ggcaacaggt ggggatttag cggagtcagt ccgcttcgcc
    12661 agcggtgtag cggcgtcgaa atgcacgcgt cccggtggac gagccgggat ccctgactgt
    12721 gatcaaaccc gatctttttt gtcacttttt gtataaaatg ccagggtgat ggtttttcga
    12781 ggaattttca tgagccttac cgaactaacc ggtaacccgc ggcacgacca actcctcatg
    12841 ctgatcgccg agcgtgggta tatgaatatt gatgagctgg caaatctgct ggatgtctcc
    12901 acgcagacgg tgcgccggga tattcgtaaa ttaagcgagc aaggcctgat tacgcgccat
    12961 cacggtggcg cgggtcgggc ttccagcgtc gttaatacgg cgttcgagca gcgtgaggtt
    13021 tcgcaaaccg aggaaaaaaa agcgattgcc gaagcggtgg cagactatat tcctgatggt
    13081 tcaacaatat ttatcaccat tggtacgacc gttgagcatg ttgcccgggc gttacttaac
    13141 cataatcatt tgcggataat caccaacagc ctgcgtgtgg cgcatattct ttaccacaac
    13201 ccgcgctttg aagtgatggt acccggcggt acgttgcgct ctcataatag cgggatcatt
    13261 ggcccttcag cggcgtcctt tgtggccgat tttcgtgctg attatctggt aacaagcgtt
    13321 ggggcgattg agagcgatgg cgcgttgatg gagtttgatg taaacgaagc taacgtggtg
    13381 aaaacgatga tggcgcacgc gagaaatatc ctgctggttg ccgatcacac taagtatcat
    13441 gcttcggcgg cggttgaaat tggtaacgtg gcacaggtca ccgcgctctt taccgacgag
    13501 ctgccgcccg ctgcgctaaa atcacgctta caagacagcc aaattgaaat catccttccc
    13561 caggaagacg cgtagatttt tgtgactgtt gacctggctt cataccttgc cacatagcca
    13621 aacccatcct ttcccgctac agttaatttc ttgtggcgcg aaaggaggca aaaatgctct
    13681 atatctttga tttaggtaat gtgattgtcg atatcgactt taaccgtgtg ctgggaacct
    13741 ggagcgattt aacgcgtatt ccgctggcta cgcttaagaa gagttttcat atgggggagg
    13801 cgtttcatca gcatgagcgt ggggaaatta gcgacgaagc gttcgcagag gcgctgtgtc
    13861 atgagatggc tctaccgcta agctacgagc agttctctca cggctggcag gcggtgtttg
    13921 ttgcgctgcg cccggaagtg atcgccatca tgcataaact gcgtgagcag gggcatcgcg
    13981 tggtggtgct ttccaatact aaccgcctgc ataccacctt ctggccggaa gaatacccgg
    14041 aaattcgtga tgctgctgac catatctatc tgtcgcaaga tctggggatg cgcaaacctg
    14101 aagctcgaat ttaccagcat gttttgcagg cggaaggttt ttcacccagc gatacggtct
    14161 ttttcgacga taacgccgat aatatagaag gagctaatca gctgggcatt accagtattc
    14221 tggtgaaaga taaaaccacc atcccggact atttcgcgaa ggtgttatgc taaaaaccat
    14281 tcaggacaaa gccaggcacc gtacccgtcc actatgggcc tggctaaaac tactctggca
    14341 acgcattgat gaggacaata tgacaaccct ggcaggtaac cttgcctatg tgtcgttgct
    14401 ctcattagtg ccgctggttg ccgttgtttt tgcgcttttc gccgcttttc ccatgttttc
    14461 cgacgtcagc attcagttac gtcactttat ttttgccaac tttctgcctg ctactggcga
    14521 tgttattcag cggtatatcg aacaatttgt tgccaattcc aacaagatga ccgccgttgg
    14581 ggcgtgcggg ctgatcgtca ccgcgttatt gttgatgtac tccatcgata gcgcgttgaa
    14641 taccatctgg cgcagtaaac gagcgcgacc caaaatttac tcgtttgccg tgtactggat
    14701 gattttaacg ctggggccgc tgctggcagg ggccagtctg gcgatcagtt cctatttgct
    14761 ctccctgcgc tgggcgagcg atctcaatac tgtcatcgac aacgtgctgc gtatttttcc
    14821 gttgctgttg tcgtggatct ccttctggtt gctgtacagc attgttccta ccatccgcgt
    14881 acctaaccgc gacgcgattg tcggcgcgtt tgttgctgca ctcctgttcg aagcaggaaa
    14941 gaaaggtttc gcgctttata tcaccatgtt cccgtcatat cagctcattt acggtgtgct
    15001 ggcggtgatc cccattctct ttgtttgggt ctactggaca tggtgtatcg tcttgcttgg
    15061 cgcggaaatt actgtcactc tcggggaata ccgcaaacta aaacaagcag ctgaacaaga
    15121 agaagacgac gaaccatgat tgcattaatt caacgcgtaa cccgtgccag cgtcaccgtg
    15181 gagggagaag tgacgggcga aattggtgcg ggacttttgg tgttattggg tgtcgaaaag
    15241 gatgacgacg aacagaaagc aaaccgtctg tgcgagcgtg tgctcggcta ccgcatcttt
    15301 agcgatgccg aaggcaagat gaatctcaac gtgcaacagg cgggcggcag tgtgctggtg
    15361 gtttcccagt ttaccctcgc cgcagatacc gaacggggga tgcgcccaag tttctccaaa
    15421 ggtgcatcac cggatcgcgc agaggcgtta tatgactatt tcgtcgaacg ctgccgtcag
    15481 caagagatga acacgcaaac aggacgcttc gctgcggata tgcaggtatc gctggtcaat
    15541 gatggccccg tgacattctg gttgcaggta tgagccagct tccagggttg tcacgggaaa
    15601 caagagagag tatcgctatg tatcaccttc gggttccaca aacagaagaa gaattagagc
    15661 gttactatca gtttcgctgg gaaatgttgc gtaagcccct gcatcaacca aaaggttcgg
    15721 aacgcgacgc gtgggatgcg atggcgcatc accagatggt cgtcgacgag cagggtaatc
    15781 tggtggcggt aggccgactg tatattaatg ccgacaatga agcgtccatt cgctttatgg
    15841 ccgttcatcc cgacgtgcag gacaaagggt taggcacgct gatggcgatg accctggagt
    15901 cggtggcgcg tcaggaaggc gttaagcgcg tgacctgtag cgcccgtgaa gacgcggtgg
    15961 agtttttcgc caagctgggg tttgttaatc agggagaaat caccacgcca accaccacgc
    16021 cgattcgcca ttttttgatg attaagcccg tcgccactct ggatgatatt ttgcatcgcg
    16081 gcgactggtg cgcgcagctg caacaggcgt ggtacgaaca tatcccgctt agtgaaaaaa
    16141 tgggcgtgcg cattcagcaa tataccgggc aaaaatttat cactaccatg ccagaaaccg
    16201 gcaatcagaa tccgcaccat acgctgtttg ccgggagttt attctcactg gcgacgctca
    16261 ccggttgggg gcttatctgg ctgatgctgc gcgaacgcca cctcggcgga acgattattc
    16321 tggcggatgc gcatatccgc tacagcaaac cgattagcgg taaaccccat gcggtagccg
    16381 accttggtgc cttaagcggc gatctcgacc gtctggcgcg cggacgaaaa gcacgggtgc
    16441 agatgcaggt cgaaatcttt ggcgacgaga cgccgggtgc agtgtttgaa ggcacgtata
    16501 tcgttctgcc cgcgaagcca tttggcccgt atgaagaggg cgggaacgaa gaagagtagc
    16561 tgatggagag gggcggtgct gcctctctca ttcaggtcat ttatattagt ccttggctat
    16621 ttcggttata ttgaataaga actctataaa tttttattct tcgttggctc catacgtgtc
    16681 ttgctacatc actgttcagg agcatttaaa tatt
//
LOCUS       DAZFAB010000097        16672 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZFAB010000097 DAZFAB010000000
VERSION     DAZFAB010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16672
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.097"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            258..1028
                     /gene="yjjP"
                     /locus_tag="ACHJWA_003647"
     CDS             258..1028
                     /gene="yjjP"
                     /locus_tag="ACHJWA_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313351.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFO4310167.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQHPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1019..1492
                     /gene="yjjB"
                     /locus_tag="ACHJWA_003648"
     CDS             1019..1492
                     /gene="yjjB"
                     /locus_tag="ACHJWA_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFO4310168.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1599..2138
                     /gene="dnaT"
                     /locus_tag="ACHJWA_003649"
     CDS             1599..2138
                     /gene="dnaT"
                     /locus_tag="ACHJWA_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFO4310169.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVDLDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2141..2878
                     /gene="dnaC"
                     /locus_tag="ACHJWA_003650"
     CDS             2141..2878
                     /gene="dnaC"
                     /locus_tag="ACHJWA_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFO4310170.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2927..3421
                     /gene="yjjA"
                     /locus_tag="ACHJWA_003651"
     CDS             2927..3421
                     /gene="yjjA"
                     /locus_tag="ACHJWA_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFO4310171.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDVENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3675..5966
                     /gene="opgB"
                     /locus_tag="ACHJWA_003652"
     CDS             3675..5966
                     /gene="opgB"
                     /locus_tag="ACHJWA_003652"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFO4310172.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6103..7125)
                     /gene="lgoD"
                     /locus_tag="ACHJWA_003653"
     CDS             complement(6103..7125)
                     /gene="lgoD"
                     /locus_tag="ACHJWA_003653"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HFO4310173.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITAGMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7264..8178
                     /gene="lgoR"
                     /locus_tag="ACHJWA_003654"
     CDS             7264..8178
                     /gene="lgoR"
                     /locus_tag="ACHJWA_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFO4310174.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8393..9754
                     /gene="lgoT"
                     /locus_tag="ACHJWA_003655"
     CDS             8393..9754
                     /gene="lgoT"
                     /locus_tag="ACHJWA_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4310175.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9803..11467)
                     /gene="tsr"
                     /locus_tag="ACHJWA_003656"
     CDS             complement(9803..11467)
                     /gene="tsr"
                     /locus_tag="ACHJWA_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFO4310176.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            11696..12238
                     /locus_tag="ACHJWA_003657"
     CDS             11696..12238
                     /locus_tag="ACHJWA_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310177.1"
                     /translation="MLNRDFDHLSTLLHLFFEREDICQKLNIIDQNNITGWEVWFQVE
                     FANMLCSTDHEWWREQALSCDMRKKPERPTLRTDFLLRKKGWAQDSYIALEIKQNRDA
                     TSCVKNMIADLEKSAKIKRSELDLRSFWTLGITPMIDRERLDSLIDCYLDEKFYLTKS
                     RKKHVLLKEINNTPYCYIVI"
     gene            12531..14681
                     /gene="btsT"
                     /locus_tag="ACHJWA_003658"
     CDS             12531..14681
                     /gene="btsT"
                     /locus_tag="ACHJWA_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFO4310178.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            14731..14934
                     /gene="yjiX"
                     /locus_tag="ACHJWA_003659"
     CDS             14731..14934
                     /gene="yjiX"
                     /locus_tag="ACHJWA_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO4310179.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            14945..15901
                     /gene="yjiA"
                     /locus_tag="ACHJWA_003660"
     CDS             14945..15901
                     /gene="yjiA"
                     /locus_tag="ACHJWA_003660"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFO4310180.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(15970..>16672)
                     /locus_tag="ACHJWA_003661"
     CDS             complement(15970..>16672)
                     /locus_tag="ACHJWA_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease family protein"
                     /protein_id="HFO4310181.1"
                     /translation="ASLSNLGVDEWIPPVMAFMNRMARTEDFNLDDFSQFITAFEKVY
                     MHGWLKKQIKSQREMVCYSALVAINNDMPFDSVINQINQHADNSGFIAALDEDLYEPR
                     PNQVNLIKAILLRLDMEQQDESVIKTYTGRITIEHILPQALVNEYWINRFQPQEHVYW
                     LHKIGNLTLISGSKNSEAQHYDFIKKKSIYEKLNSKSSFDLTKDVCNSSEWGLAELKM
                     RHEKMKTQLKKLWLV"
BASE COUNT         4066 a         4331 c         4029 g         4246 t
ORIGIN      
        1 ctaaaaaacg cgcccagcta tctattatcc tgacccgtaa aggctaaaac aataaattca
       61 ttatagtgat aatattcatg tgatagtttg tttgcggcga gagataattc gctttttatc
      121 accgagcttg tcccgataac caggattaat cagcgcaatt ttgatcttaa cctaccgcaa
      181 aacaaccttg cttcatgata caaatggatt ctcattatct gaacaatacg cagcacgtct
      241 atgacaaagg gcgagttatg caaactgagc aacagcgagc cgtaacacgg ctttgtatcc
      301 agtgtggatt atttcttttg caacatggtg cggaaagcgc gttggttgat gagctttcct
      361 cacgactggg tcgggcgctg ggaatggaca gcgtcgaaag ttctatctct tcgaacgcca
      421 tagtgctgac aactattaaa gatgggcaat gcctgacatc gacacgtaaa aatcacgatc
      481 gcggcattaa tatgcatgtg gtgactgaag tccagcacat tgtgattctt gcggagcatc
      541 atctgctgga ttacaaaggc gtagagaaac gatttagcca aattcagcca ttacgttacc
      601 caagatggct ggtagcctta atggttggcc tttcttgcgc ctgtttctgt aaactcaata
      661 acggtggctg ggatggtgcc gtcatcacct tctttgccag tacgaccgcg atgtatatcc
      721 gccagctgct ggcacaacgt catcttcatc cacagatcaa cttttgcctt accgctttcg
      781 ccgccaccac catttccgga ttgcttttgc aacaccccac tttcagcaat acccccacca
      841 ttgcgatggc cgccagcgtt ctgctgctag tgccgggctt tccgttgatt aatgccgtcg
      901 ccgatatgtt taaaggccac atcaataccg gactggcacg ctgggcgatc gccagtctgc
      961 tgacactggc tacctgcgtc ggcgtagtga tggcactgac gatttggggg ctacgcggat
     1021 gggtgtgatc gaatttctgt tagcgttggc gcaggatatg atcctcgccg ccattcctgc
     1081 ggtcggcttt gcgatggtgt tcaacgttcc cgtgcgggcg ttacgctggt gtgcgctgct
     1141 tggctcgata ggtcatggtt cccgaatgat cttgatgacc agcgggttga atattgagtg
     1201 gtcaaccttt atggcctcca tgctggtcgg caccattggt attcaatggt cgcgctggta
     1261 tctggcgcat ccgaaagtgt ttaccgtggc ggccgttatc cctatgttcc cgggcatatc
     1321 ggcttatacc gcaatgattt cggcggtaaa aatcagccag ttaggttaca gcgaaccgtt
     1381 gatgattacc ctgttaacca actttcttac agcttcatcg attgttggtg cgttatccat
     1441 cggtctttcc attcctggat tatggttgta ccgcaagcgc cctcgcgtat aaaattgcct
     1501 cgcttaagca cacggatgag agacagcctc ctctcctccg tgtgttacta taaaagttat
     1561 ctcccttctc gttcatcgtt ccatatttga gaaacagtat gtcttccaga gttttgaccc
     1621 cggacgtcgt tggtattgac gccctggtac acgatcacca aaccgttctg gcaaaagctg
     1681 aaggcggtgt ggttgccgta tttgctaaca atgccccggc gttttatgcc gtcacgcctg
     1741 cacgcctggc tgaactgctg gcgctggaag aaaagctggc gcgtccggga agcgatgtcg
     1801 atctggacga tcaactctat caggaaccgc aagccgctcc cgttgctgta cccatgggga
     1861 aattcgccat gtatccggac tggcaacccg atgccgactt tatccgcctg gcggctctat
     1921 ggggcgtggc gctaagagag ccggtaacca ccgaagaact ggcctcattc attgcctact
     1981 ggcaggcgga aggtaaagtc tttcaccatg tgcagtggca acaaaaactg gcgcgcagcc
     2041 tgcaaatcgg tcgtgccagc aacggcggac tgccgaaacg agatgtgaat acggtcagtg
     2101 aacctgacag ccaaattcca ccaggattca gagggtaacg atgaaaaacg ttggcgacct
     2161 gatgcaacgc ctgcaaaaaa tgatgcctgc ccatatcaaa cctgcattca aaacgggtga
     2221 agagctactg gcctggcaga aagaacaagg ggcgatccgc tccgccgctc tcgaacgtga
     2281 aaatcgggcg atgaaaatgc agcgcacctt taaccgctcc ggtattcgcc cactgcatca
     2341 gaactgctcc tttgagaact atcgcgttga gtgtgaaggg cagatgaatg cgttaagcaa
     2401 agcgcgccag tatgtcgaag agtttgacgg caacatcgcc agcttcatct tttctggtaa
     2461 gccaggaacc ggcaaaaacc atctggcggc ggcaatctgc aacgagctgc tgctacgcgg
     2521 taaatccgta ttgatcatca ccgtggccga tattatgtcg gcgatgaaag ataccttcag
     2581 gaatagcggt accagcgaag aacaactgct taacgatctg agcaacgttg atctgctggt
     2641 gatcgatgag atcggcgtgc agaccgaatc gaaatacgaa aaagtgatca tcaaccagat
     2701 cgtcgatcgc cgctcttctt ccaaacgccc aaccgggatg ctgaccaaca gtaatatgga
     2761 agagatgacc aagttgctgg gcgaacgcgt gatggaccgt atgcgcctgg gtaacagttt
     2821 gtgggtgatc ttcaactggg atagctaccg tagccgtgta acaggtaaag agtattaatc
     2881 cccgtttctg gcggtatact gcgacacaat tccaccttga gtaatgatga tgaaaactgt
     2941 taaacatctt ctgtgctgtg ccattgccgc cagcgcatta atttccaccg gggtgcatgc
     3001 tgcgtcctgg aaagatgcgc tctccagcgc cgccagcgaa cttggcaacc aaaacagcac
     3061 gacacaggaa ggcggttggt cgctcgcgtc attaactaac ttgcttagca gcggaaacca
     3121 ggccttaagc gcagataaca tgaacaatgc cgcaggcatt ctgcaatact gcgcgaagca
     3181 aaagctggct tcggtaaccg atgtcgaaaa catcaagaac caggtgctgg aaaagctggg
     3241 cctgaacagt gaagagcaaa aagaagacac caactatctg gacggcattc agggtttgct
     3301 gaaaaccaaa gatggtcagc aactcaatct ggataacatc ggaacaactc cgctggcaga
     3361 aaaggtgaaa accaaagcct gcgatctggt gttaaagcag gggctgaact tcatttcctg
     3421 aaccgcgaaa cgccgcgttt tagctgttac ccggctgacg cggctctctc ctgccctttt
     3481 ccctgctcct ggacggtttt ccccctgtta cacgggaagc gaccgcgatc aaaaaagtct
     3541 gtttataaac taattcctaa caacatcgca tttttttgac actagaattg cagcaatatg
     3601 gcgacgccat tacattgtgc ttctcaaaaa gcttaaacgt tggctaaacg gcccggttta
     3661 gtgaggatcg cccgttgtca gaactactct ctttcgccct ttttctcgcc tctgtgctga
     3721 tttacgcatg gaaagcggga cgtaacacct ggtggtttgc agccacatta acggtgctgg
     3781 ggctatttgt cgttttaaat atcaccctgt ttgccagcga ctattttact ggcgatggta
     3841 ttaacgacgc ggttctctat accttaacca acagcctgac cggtgctggc gtcagcaaat
     3901 acattttgcc gggtatcggc attgtgctgg ggctgacagc ggtgttcggt gcgctgggct
     3961 ggatcctgcg ccgtcgtcgc catcatccgc accattttgg ttacagcctg ctggcgctct
     4021 tactggcgct gggttcggtg gacgccagcc cggcatttcg tcagataacg gaactggtga
     4081 aatcccagtc acgcgacggc gacccggact ttgcggctta ttataaagag ccgtcgaaaa
     4141 ctatccctga cccgaaactc aacctggttt atatctacgg cgaaagtctc gagcggacct
     4201 attttgataa cgaggctttc ccggacctca cgcctgaact gggcgcgttg aaaaatgaag
     4261 gcctggattt cagccacacg cagcagctgc cgggaacaga ttacacgatt gcgggcatgg
     4321 tggcttctca gtgcggcata ccgctgtttg ccccctttga aggcaacgcc tccgcttctg
     4381 tttccagctt cttcccgcag aacatctgtc tgggcgatat cctgaaaaac tcgggttatc
     4441 agaactattt cgtgcagggc gcgaatctgc gttttgccgg taaagatgtg ttcctgaagt
     4501 cgcatggctt cgaccactta tacggctcag aagagctgaa aagcgtggta gccgacccgc
     4561 actatcgcaa cgactgggga ttctacgacg ataccgttct cgatgaagcg tggaaaaagt
     4621 ttgaagagct ttcccgctca ggtcagcgat tctcactgtt taccctgaca gtcgataccc
     4681 atcacccgga tggttttatc tctcgtacct gtaaccgcaa aaaatatgat tttgacggta
     4741 aaccgaatca gtcattcagc gcggtaagtt gcagccagga gaatatcgcg acgtttatca
     4801 acaaaatcaa agcgtcaccg tggtttaaag atactgtcat cgtcgtctct tctgaccatt
     4861 tagcgatgaa caacacggcg tggaaatacc tcaataagca ggatcgcaat aacctgtttt
     4921 ttgtcattcg tggcgacaag ccgcagcaag agacgctggc agtgaagcgt aacacgatgg
     4981 ataacggagc gacagtgctg gacattctcg gtggcgataa ctatctcgga cttggtcgta
     5041 gcagtttatc cgggcagtca atgtcggaaa tcttcctcaa tatcaaagag aaaacattgg
     5101 cgtggaagcc ggatatcatc cgtctgtgga aattccctaa agagatgaaa gagttcacca
     5161 tcgaccagca gaaaaacatg attgccttct cgggtagcca tttccgtttg ccgctgctgt
     5221 tgcgggtttc agacaaacgc gtggaaccgc tgccagaaag cgaatactca gcaccgctgc
     5281 gtttccagct ggccgatttc gctccacgcg acaatttcgt ctgggttgac cgttgctaca
     5341 agatggcaca actctgggct ccggaactgg cactttccac cgactggtgt gtctcgcagg
     5401 ggcaacttgg cggtcagcaa attgttcagc atgttgacaa aacaacatgg aagagcaaga
     5461 cggcatttaa agatacggtc atcgacatgg cgcgttacaa aggcaatgtc gatacgctga
     5521 agattgttga taacgatatt cgctacaaag ccgacagttt catctttaac gtcgccggtg
     5581 cgccagaaga ggtgaaacag tttagcggga tttcccgtcc ggagtcgtgg ggccgctggt
     5641 ccaacgcgca gctgggcgat gaagtaaaaa tcgagtacaa gcatccgctg ccgaagaaat
     5701 ttgacctggt gattaccgcc aaagcatacg gcaataacgc cagccgtcct attccggtac
     5761 gcgtaggcaa tgaagaacaa actcttgtgc tgggcaatga agtgaccacc accacactgc
     5821 atttcgataa cccaaccgat gccgacacac tggtaattgt gccgccggaa cctgtctcaa
     5881 ccaacgaagg gaatatcctc ggacactcgc cgcgtaagct cgggatcggc atggtggaaa
     5941 ttaaagtggt agaacgtgaa gggtaattcc tgactgatgt gtaggtcgca tagggtgcga
     6001 cctacacacc ttctccggct tacaacacat tattaagaaa caaaaaaaac agcgcccaac
     6061 acttccccgg acgctgcatg gcaactttaa taatctcgcc tttcagaaag taattacgcc
     6121 tttaattaac tcacgattgt taatcacatc gcgctcgtag gtttctgcca gcgtggcgaa
     6181 cggatagcga tgggttaaca tcatgccagc agtgattttt ccttccgcca tcagtcgacc
     6241 gactttagca aaatcttccg gcgtggcgtt gcggctgccc atcatcgtcg tttctttttt
     6301 atggaattcc ggatcggaga actgcaactc acctttaaac aggccgacaa ataccaccgt
     6361 gccgccgtga cgaatcagat tcacggtgtt attcatcgca tgttgattac ctgtcgcgtc
     6421 gatcactttc tgcgccagcg aaccaccaaa ctgcgcccgc agctgcgcgt caaaatcttc
     6481 ggctgacggg tccagcacgg gcaactccag acgcgttgcc acatgttcac ggcgcgccgg
     6541 actggtatcc gccaccacca cctgtgcgcc atcggcttta gcgattgccg ccgcgcccag
     6601 accgattggc ccggccccga ccaccagcac ctgctcgccg ggagcaatgg ctgcgcgacg
     6661 caccgcatga gcgctaatag cgaaaggttc aatcaatgcg gccgcctgcg ggtcaatacc
     6721 gtctgcgggc aaaatgttcg ccaccggcac gctcaaatac tcactaaaac cgccatcctg
     6781 atgcacgcca atgactgaaa ttttttcaca gcaattggta cgcccgcttt tacacgccgg
     6841 gcattgctga caggcaacat aagggatcac agcaacttgc tgaccatttt taagatcagc
     6901 aatattttta cccagcccaa caatctcccc acatatttca tggcctaaaa cccgtggata
     6961 actaaaaaat ggttgatttc caccccaggc atgaatatcg gtaccgcaaa tcccgacaga
     7021 cttaattttt attaatgctt cattgtcacc cggaataggt atctcgcgtt gtttccagac
     7081 taattctttc ggctgctggc aaattaaaac attcatcgta gacatgattc ggctctccat
     7141 tttttgttgc cttagatatc aatgaaaatg aaaaatggca gcggaacgga aaatctgttt
     7201 tgtgaaaaca cacgcataaa aatgttttaa atcgggtttt aatggactca caaggaggaa
     7261 aatatgagtc gttcacaaaa tttacgccac aatgtgatta accaggtcat tgatgatatg
     7321 gcgcggggtc atatcccttc cccacttcca tcgcaaagtg cgctggcgga aatgtacaac
     7381 atcagccgca ctacagtgcg tcatattctc agccacttac gcgaatgcgg cgtcttaacg
     7441 caggttggaa acgactatgt tattgcgcgt aaacctgacc atgatgacgg tttcgcctgt
     7501 actaccgctt caatgagtga acaaaacaaa gtgtttgagc aggctttttt cacgatgata
     7561 aaccagcgcc agttacgccc aggggaaact ttttctgaac tgcaactggc tcgggccgcg
     7621 ggtgtcagcc cggtggttgt aagagaatat cttttaaaat tcggccgtta taatcttatt
     7681 caaagcgaaa agcgcggcca gtggagcatg aagcaattcg atcaatccta cgctgagcag
     7741 ctgtttgagc tacgcgaaat gctcgaaaca cattcacttc agcactttct caacctgcct
     7801 gatcatgatc cccgctggct gcaggcgaaa accatgctcg aacgtcatcg gctactgcgt
     7861 gacaacatcg gcaatagctt tcgcatgttc tcgcaacttg acagagattt tcactcctta
     7921 ctgctttcag ctgccgacaa tatctttttt gatcaatcgc ttgagatcat ctcagtcatc
     7981 tttcattttc actatcaatg ggacgaaagc gatcttaaac agcgcaatat cattgccgtc
     8041 gatgagcata tgaccattct cagcgctctg atttgccgca gtgaccttga tgccaccctt
     8101 gcgctccgta accatttgaa ttcagcgaaa caatcaatga ttcgatccat taatgagaac
     8161 acgcgttatg ctcattaagt acggattaaa aacagcaaac tattagcggg tgcacggttt
     8221 tatcaaaaga taaataccgg gaaattcccg cttacctatg ctcacaatca gtcgactaaa
     8281 aatgtttttg tgagcggtag taaagtccta aaactttaac ctgccgttaa tgtcaacctt
     8341 gcgtaatgca caattacgta aacaattaaa attataaata caggagtccg gcgtggaaaa
     8401 agaaaatatc accctcgatc cgcgttcttc atttactcca tcttcgtcgg cagatattcc
     8461 cgtgccacca gatggattag ttcaacgcag tacccgaatt aaacgcattc aaaccaccgc
     8521 catgttgtta ttattttttg cggcggtaat caattatctc gaccgcagtt cgctgtcggt
     8581 agcaaattta acgattcgtg aagaattggg attaagtgcc accgaaatcg gcgctttgct
     8641 ctccgtgttt tcactcgctt acggcattgc gcaacttcct tgcggcccac tattggatcg
     8701 taaaggccca cgcctgatgc tgggactggg gatgttcttc tggtcactgt tccaggccat
     8761 gtctggcatg gtgcacagct ttacgcagtt cgtgttggtg cgtatcggta tggggattgg
     8821 tgaagcgccg atgaacccat gcggtgtaaa agtcattaac gactggttca acatcaaaga
     8881 gcgcggacgc ccgatgggct tcttcaacgc agcttctacc attggcgttg ccgtaagccc
     8941 accgattctg gcggcgatga tgctggtgat gggctggcgc gggatgttta ttaccattgg
     9001 tgtactgggg atttttctcg ccatcggctg gtatatgctc tatcgcaacc gcgagcacgt
     9061 agaactgact gccgttgaac aagcttatct caatgcaggt agcgtcaatg cccgccgaga
     9121 tccgctcagt tttgccgaat ggcgcagcct gttccgtaac cgcacaatgt ggggaatgat
     9181 gctcggattc agtggcatca actacactgc gtggctgtat ctggcctggc ttcctggtta
     9241 cctgcaaaca gcctataacc tggatttaaa aagcacaggg ttgatggcgg ctatcccttt
     9301 cctgtttggt gctgccggga tgctggtcaa cggttacgtt actgactggc tggtcaaagg
     9361 gggaatggct ccgattaaaa gccgtaagat ctgcattatt gccgggatgt tctgttctgc
     9421 cgcctttacg ctgatagtac cacaagcgac aacatccatg atggcggttc tgctgattgg
     9481 catggcactg ttctgtattc actttgccgg aacatcctgc tggggcttga tccacgtcgc
     9541 agttgcttct cgcatgactg cgtcggtggg cagtatccag aactttgcca gcttcatctg
     9601 cgcctctttc gcgccgatca ttactggttt tattgttgat acaacccact cattccgtct
     9661 ggcactaatc atttgcggtt gcgtcaccgc agcgggagca ctggcgtaca tcttcctggt
     9721 tcgtcagccg atcaacgacc cgcgtaaaga ttaaactccc tatgcccgat aacattttgc
     9781 ttatcgggca tttttatggc gattaaaacg tttcccagtt ctccccgcta tctgccactg
     9841 ccattttacg cggcgtagct ggcgtcacgg tttttaccac agccgatgtt tcacgctgct
     9901 gttgctgctg ttgctgaatc cggaacactg ccacagcttc ggtcaggcga ctggcctgct
     9961 cttccaacgc ggcggcggca gcggcagact cttccaccag cgcagcgttc tgttgagtta
    10021 cccggtccat ctcagcaacc gctaagccaa cctgatcgat accacggctc tgctcatcag
    10081 aagcagaagc tatttcgccc ataatgtccg tcacgcgggt cacagcactg acaatctccg
    10141 ccattgtttc cccggcgctt tcgaccagcg tagagccaac atccactttc cccaccgagt
    10201 cttcaatcag gcttttaatt tcacgagccg cctgggcgct acgctgggcc agattacgca
    10261 cttctcccgc aaccaccgca aaaccacgtc cttgctcacc cgcacgcgct gcttcaaccg
    10321 ccgcgttcaa agccagaata ttggtctgga aggcaatgcc gtcaattacg ctgataatat
    10381 cggcgatttt ctgcgaactg gtggagatat cacgcatagt ctgcaccacg ttatctacca
    10441 ctttgccgcc gcgttgcgcc gtttcagaag cacttaacgc caggtggctg gcctggcgcg
    10501 cattctcggc gttctgtttc accgttgcgg tgagttgctc catgctggct gccgtctctt
    10561 ccagcgaagc ggcctgttgc tcggtgcgcg aagagagatc gttattgccg gtagcgattt
    10621 cgctggcacc gctatagatg gcattggccc cgttgcgcac atcaccgacg gtacgcatca
    10681 gctctccctg catatggcgc aaactctctg ccagttgccc catctcatta gagccatcca
    10741 cctcaatcgg tttcaccaga tcgccgcctg caatatgacg aatgctgtca atcaggcgat
    10801 tcattggcgc taccagcgag gctttaatac cgaaccagac ggcgaagatg accgccagta
    10861 cgacgatcat cacgcccacc agaatccaca tcgcctggct gtaggaggca ttgttatcgc
    10921 tgacggcgat atcatagagc cgatcgtttt gctccatgta agccacatac tgcttctcga
    10981 aaccgtcctg atatccctgg gtcggctgat caaagaactc gttgattttg cctgcaccta
    11041 acagttggat cagctccgcc agcgcattgt gataaatatc gtaattacgt ttgatctctg
    11101 ccgctgcggc ggtgctctga cgcgggtcac gcggcaacgc ttcgtaatct gcccagtttt
    11161 tttccgcctg tttcaacgaa atactggcac tctgcatcag ctcagcaacg gttgaaccgc
    11221 taccaatatt attctgatcc atcatgtagc ggatacccgc gcggttgagg gtgttacgcg
    11281 tctgcaacaa cgcgacccag ctgccattca gcgtggattg ctgctggcga atggtttgta
    11341 aaacagtgaa attttctttg tcattcttta aggcattaaa gaacagaccg cctgatgtca
    11401 gttgtaaaag gccaaaaacg gccaaaacca gcagtaagct ggtcacgatc ttgatacgtt
    11461 ttaacatggt ttctctttcc tgtggaccga tgcaaaattt tcggcctgaa atgagaaaac
    11521 tttataggta gcgacttgaa actggttaat tttacctcac tttttttaat ttttgttcaa
    11581 aaatacaata accacttatt gatacatttt cttttcttat ttttttcaca cgaattgtat
    11641 ctattttgaa cgaaatattt tatagagtgt ggaattttaa gaaaggacat ctgaaatgtt
    11701 aaaccgtgac ttcgatcatc tctcaacgct attacatctc ttttttgaac gtgaggatat
    11761 ttgccaaaag cttaatatta ttgaccaaaa caatatcacc ggttgggaag tgtggttcca
    11821 ggtagagttt gctaatatgc tctgctcaac agaccatgaa tggtggcgtg aacaagcact
    11881 ttcctgtgat atgcgcaaaa aacctgagcg cccgacacta cgcacagatt tcttactacg
    11941 taaaaaaggc tgggcacaag atagctatat cgcgcttgaa ataaaacaaa acagagacgc
    12001 gaccagttgt gttaaaaata tgatcgcaga cttagaaaaa agcgcaaaaa taaaacgttc
    12061 cgaactcgat ttacgcagtt tctggacctt gggtatcacc cctatgatag acagggaaag
    12121 gctggactct ctcatcgatt gctatttaga tgagaaattt tatcttacta aatctcgtaa
    12181 aaaacacgtt ttacttaaag aaataaataa cacaccatat tgctatattg ttatttaata
    12241 tttatagtat gaatataatt tttaaatgag ttacacaacc gagggctaaa aatcgcttcg
    12301 gttgtgatca tcatcacata aataaccttc ataaaccttg ctcaaaaccg ctcaaccgca
    12361 aaactgaccg cttacgtccc taaaataacc actcagttat ttaccttact ttacgcgcgc
    12421 gtaactctgg cagcatcatt gcaggatagc ggtcaattta cctcctcaaa cgcaacgcaa
    12481 acctagaacg gcttcggcca actattaatc aatacatgcc aggttttact atggatacga
    12541 aaaaactatt caagcacata ccctgggtga ttctcggaat catcggtgca ttctgcctcg
    12601 cggtagttgc attacgtcgg ggggagcacg tcagcgccct gtggatcgtg gtcgcctctg
    12661 tgtcggtgta tctggtggcg tatcgctact acagtctgta catcgcccag aaggtgatga
    12721 aactcgaccc cacgcgcgcg acgcctgcgg ttattaacaa cgacggtctg aactacgttc
    12781 cgaccaaccg ttacgtgttg tttggtcacc acttcgccgc tatcgccggt gctggtccgc
    12841 tggtgggacc ggttctcgcc gcgcagatgg gctacctgcc tggcacgctg tggctgctgg
    12901 cgggggtagt gctggccggt gcggttcagg actttatggt gctgtttatc tcctctcgcc
    12961 gtaacggcgc atctctcggt gagatgatca aagaagagat ggggccagta ccggggacta
    13021 tcgcgctgtt tggctgtttc ttaatcatga tcatcatcct cgccgtcctg gcgctgattg
    13081 tggttaaagc cctggcagaa agtccgtggg gtgtcttcac cgtttgctca accgtaccaa
    13141 ttgcgctgtt tatgggtatc tacatgcgct ttatccgtcc ggggcgtgtg ggtgaagtct
    13201 ctgttattgg tatcgtgctg ctggttgcct ctatctactt cggtggcgtg attgctcacg
    13261 atccgtactg gggtccggca ctgaccttta aagacaccac cattaccttc gcgctgattg
    13321 gctatgcgtt tgtttccgcg ctgctgccgg tgtggctgat cctcgcacca cgcgactatc
    13381 tggcaacctt cctgaagatc ggcgttatcg tcggcctggc gctgggtatc gtggtgctga
    13441 acccggaact gaaaatgcct gcgatgaccc agtacattga cggtactggc ccgctgtgga
    13501 aaggcgctct gttcccgttc ctgttcatca ccatcgcctg tggtgcggta tctggcttcc
    13561 acgcgctgat ctcttccggt acgacgccga aactgctggc taacgaaacc gacgcgcgtt
    13621 tcatcggcta cggcgcaatg ctgatggagt ccttcgtggc gattatggcg ctggttgctg
    13681 catccatcat cgaaccgggt ctttacttcg cgatgaacac cccgcccgcg ggccttggca
    13741 tcaccatgcc taacctgcat gaaatgggtg gcgagaacgc gccgatcatc atggcgcagc
    13801 tgaaagacgt taccgcacac gcggcagcga ccgtcagctc ttggggcttc gtgatttcgc
    13861 cagagcagat cctgcaaacc gcgaaagaca ttggtgagcc ttctgtcctg aaccgtgcag
    13921 gtggtgcgcc gacgctggcg gtaggtatcg ctcatgtgtt ccacaaagtg ctgccgatgg
    13981 ctgacatggg cttctggtat cacttcggta ttctgttcga agccctgttc atcctgaccg
    14041 cgctggatgc gggtacccgt tctggccgct ttatgctgca agacctgctg ggtaacttca
    14101 tcccgttcct gaaaaaaacc gactctctgg ttgctggtat catcggtact gcgggctgtg
    14161 tgggtctgtg gggctacctg ctgtatcagg gcgtggtcga tccgctgggc ggcgttaaga
    14221 gcctgtggcc gctgttcggt atctccaacc agatgctggc agccgtagcg ctggtactgg
    14281 gcaccgttgt gctgattaag atgaagcgca cccaatacat ctgggtaact gttgttccgg
    14341 ctgtatggct gcttatctgc accacctggg cgctgggtct gaaactgttc agcaccaacc
    14401 cgcagatgga aggcttcttc tacatggcaa gccagtacaa agagaagatt gctaacggta
    14461 ctgaccttac ggcgcagcag attgccaaca tgaaccacat cgttgtgaac aactacacca
    14521 acgcaggcct gagtattctg ttcctgattg tggtgtacag catcatcttc tacggtttca
    14581 aaacctggct tgcggtacgt aacagcgaca aacgtactga caaagaaaca ccgtacgttc
    14641 caatcccgga aggcggcgtg aagatctctt cgcaccacta accgtgttta gccccgcttc
    14701 ggcggggctt tgttcttatc agagtgaact atgtttggta acttaggaca ggcaaaaaaa
    14761 tatctcggcc aggcggcgaa gatgttgatt ggcattccag actacgacaa ctacgtcgag
    14821 catatgaaga ccaaccatcc cgacaagccg tacatgagct atgaagaatt cttccgcgaa
    14881 cgccagaacg cgcgctacgg cggcgatggt aaaggcggta tgcgctgttg ttaatggaga
    14941 gagcatgaac ccgattgcag ttaccctact caccggtttt ttaggcgcgg gcaaaaccac
    15001 cctgctgcgc catattctta acgaacaaca cggctacaag attgccgtga ttgaaaacga
    15061 atttggcgaa gtctccgttg atgatcaact gattggcgac cgcgccacgc agatcaaaac
    15121 gctgaccaac ggctgcatct gctgttcgcg ctccaacgag ctggaagacg cactactcga
    15181 cctgctggat aatctcgata agggcaatat tcagttcgat cgtctggtca ttgaatgcac
    15241 cggcatggcc gatcccggcc cgattattca gacctttttc tcccatgaga ttttatgcca
    15301 gcgttacctg ctggacggcg tgattgcgct ggtggatgcg gtacatgccg atgagcagat
    15361 gaaccagttc accatcgccc agtcccaggt tggctacgcc gaccgtattc tgctgaccaa
    15421 aaccgacgtc gcaggcgaag cagaaaaact gcgcgaacgc ctggcgcgca tcaacgcccg
    15481 tgcaccggtc tacaccgtta cccacggcga catcgacctg ggcctgctgt tcaacaccaa
    15541 cggttttatg ctggaagaaa acgtcgtcag caccaaaccg cgtttccact ttatcgcgga
    15601 taaacaaaac gatatttcgt cgattgtggt agaactggat tacccggtag atatcagcga
    15661 agtttcccgc gtgatggaaa acctgctgct ggaatcggcg gataaactgc tgcgttacaa
    15721 agggatgctg tggattgacg gcgaacctaa ccgcctgctg ttccagggcg tccagcgcct
    15781 ctacagcgcc gactgggacc gcccgtgggg cgatgaaaaa ccgcatagca caatggtttt
    15841 tatcgggatt cagttgccgg aggaagagat tcgggcagcg tttgcggggt tgaggaagta
    15901 agttcgggaa agatcaactt tttgagagtt ccaccgcaac attattacga gtggaactct
    15961 ttagatatgt cagaccaacc aaagcttctt caattgcgtt ttcatttttt catgtctcat
    16021 tttcagttca gccaatcccc attctgaaga attacaaaca tcttttgtta aatcaaaaga
    16081 gcttttacta ttaagtttct cataaataga tttctttttg ataaaatcat agtgttgtgc
    16141 ttcagaattt ttggaaccac ttatgagtgt taaattacct attttatgca gccagtagac
    16201 atgctcttga ggttggaatc tattaatcca gtattcatta actaatgctt gaggaagaat
    16261 atgctcaatt gtgattctcc ccgtataggt tttaatcacg ctctcatctt gttgctccat
    16321 atctaagcga agcaaaattg cttttattaa gttcacttga ttcggacgag gctcatataa
    16381 atcttcatcc agagcagcta taaaacctga attatctgca tgttggttta tctgatttat
    16441 cacagaatca aaaggcatat cattatttat tgctaccaga gctgaataac aaaccatttc
    16501 tctttggctt ttaatttgtt tttttaacca cccatgcata tataccttct caaaggcggt
    16561 aataaattga gaaaaatcat ctaaattaaa atcttctgta cgagccattc gattcatgaa
    16621 tgccataacc ggaggaatcc actcatctac ccctaaatta cttagcgatg ct
//
LOCUS       DAZFAB010000098        16640 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZFAB010000098 DAZFAB010000000
VERSION     DAZFAB010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.098"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..182
                     /locus_tag="ACHJWA_003662"
     CDS             <1..182
                     /locus_tag="ACHJWA_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO4310182.1"
                     /translation="DKQYVWCWGKSYGWTECDTFEGYYDCSRNKWWAVTDNGEEPASK
                     VTHWMPLPEPPQEVK"
     gene            270..512
                     /locus_tag="ACHJWA_003663"
     CDS             270..512
                     /locus_tag="ACHJWA_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO4310183.1"
                     /translation="MFALIQRGQIYTDRAGYPVVITRSTQHSVFFRRMDGRSGRVRIG
                     EFNNLFEHIDQQEYRKILAGTEQEMRLKKLRAMQRR"
     gene            516..650
                     /locus_tag="ACHJWA_003664"
     CDS             516..650
                     /locus_tag="ACHJWA_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310184.1"
                     /translation="MHTAFEFWVRKTFGNRYDLTRDVDGFYCREVVKRMFDVWCHCRG
                     "
     gene            669..923
                     /gene="xisR"
                     /locus_tag="ACHJWA_003665"
     CDS             669..923
                     /gene="xisR"
                     /locus_tag="ACHJWA_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO4310185.1"
                     /translation="MQTIIYQITPSKWCTERVLIASTGLKPGTIERARRKSWMQGKEY
                     RHYAVEGDPGHYSECLYNIEEIMRWIENQKQPGAKNASSG"
     gene            957..2243
                     /locus_tag="ACHJWA_003666"
     CDS             957..2243
                     /locus_tag="ACHJWA_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4310186.1"
                     /translation="MSNASYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRMGSFDYAAQFPNSPNLKHFGLGKREITVKALSEKWLDLKKIEICAN
                     ALNRYQSVIKNMLPMLGEKKLVSSITKEDLLFVRRDLLTGYQKLSNGKTSSIKGRSVV
                     TVNYYMTTIAGMFQFATDNGYTSGNPFNGLAPLKKSKVKPDPLTRDEFIRFIEACRHQ
                     QTKNLWILAVYTGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGRT
                     IHLVQPAIDALKSQAEMTMLGKQHSVEVKQREYGRTAVHKCTFVFSPQVTKQQQLSGP
                     HYKVDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMV
                     FNVYGAWMKDNNHEQIELLNKRLSESVPCMPHKKAG"
     gene            2264..2965
                     /gene="mlrA"
                     /locus_tag="ACHJWA_003667"
     CDS             2264..2965
                     /gene="mlrA"
                     /locus_tag="ACHJWA_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFO4310187.1"
                     /translation="MSLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYP
                     AQTLTTHLFIPLRRRLQYQQPTLQALLAILDGVLINYIAICLASARKKQGKDALVVGW
                     NIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQL
                     TSWQEQGHDIFPLGI"
     gene            3025..3132
                     /gene="yohO"
                     /locus_tag="ACHJWA_003668"
     CDS             3025..3132
                     /gene="yohO"
                     /locus_tag="ACHJWA_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFO4310188.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(3113..3844)
                     /gene="yehW"
                     /locus_tag="ACHJWA_003669"
     CDS             complement(3113..3844)
                     /gene="yehW"
                     /locus_tag="ACHJWA_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFO4310189.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(3849..4775)
                     /gene="yehX"
                     /locus_tag="ACHJWA_003670"
     CDS             complement(3849..4775)
                     /gene="yehX"
                     /locus_tag="ACHJWA_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFO4310190.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGELRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPSGTLHFQDLLVEA"
     gene            complement(4768..5925)
                     /gene="yehY"
                     /locus_tag="ACHJWA_003671"
     CDS             complement(4768..5925)
                     /gene="yehY"
                     /locus_tag="ACHJWA_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFO4310191.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLASALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAGLVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            complement(5932..6849)
                     /gene="osmF"
                     /locus_tag="ACHJWA_003672"
     CDS             complement(5932..6849)
                     /gene="osmF"
                     /locus_tag="ACHJWA_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFO4310192.1"
                     /translation="MPLSKVWAGSLVLLAAVSLPLHAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLRRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGSDGPVAAQGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(7101..9398)
                     /gene="bglX"
                     /locus_tag="ACHJWA_003673"
     CDS             complement(7101..9398)
                     /gene="bglX"
                     /locus_tag="ACHJWA_003673"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFO4310193.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITILQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            9594..11309
                     /gene="dld"
                     /locus_tag="ACHJWA_003674"
     CDS             9594..11309
                     /gene="dld"
                     /locus_tag="ACHJWA_003674"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO4310194.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGHG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(11347..12279)
                     /gene="pbpG"
                     /locus_tag="ACHJWA_003675"
     CDS             complement(11347..12279)
                     /gene="pbpG"
                     /locus_tag="ACHJWA_003675"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFO4310195.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(12453..13040)
                     /gene="yohC"
                     /locus_tag="ACHJWA_003676"
     CDS             complement(12453..13040)
                     /gene="yohC"
                     /locus_tag="ACHJWA_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFO4310196.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            13210..13788
                     /gene="yohD"
                     /locus_tag="ACHJWA_003677"
     CDS             13210..13788
                     /gene="yohD"
                     /locus_tag="ACHJWA_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO4310197.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGLAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(13918..14679)
                     /gene="yohF"
                     /locus_tag="ACHJWA_003678"
     CDS             complement(13918..14679)
                     /gene="yohF"
                     /locus_tag="ACHJWA_003678"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO4310198.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGNLPEGAQALEKLIQRLGRIDVLVNNAGAMTKVPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(14732..16168)
                     /gene="mdtQ"
                     /locus_tag="ACHJWA_003679"
     CDS             complement(14732..16168)
                     /gene="mdtQ"
                     /locus_tag="ACHJWA_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFO4310199.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLIALTNHQTKSLTLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            16392..16475
                     /gene="yohP"
                     /locus_tag="ACHJWA_003680"
     CDS             16392..16475
                     /gene="yohP"
                     /locus_tag="ACHJWA_003680"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFO4310200.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
BASE COUNT         4106 a         4462 c         4199 g         3873 t
ORIGIN      
        1 acgataaaca gtatgtttgg tgttggggga agtcttacgg ctggactgag tgcgatacct
       61 tcgaagggta ttacgattgt tcgagaaaca aatggtgggc agttactgac aatggggaag
      121 aaccggcatc gaaagtaacc cactggatgc cgctaccgga gccgccgcag gaggtgaaat
      181 gatgaattgg cctgaagcat tcaccgcagt tggagttgca atagcggtgg catttattct
      241 gtattcgctt ttccgctggg gataaaggaa tgttcgctct gattcaacgt ggtcagatat
      301 acacggacag agccggatac cctgtggtga ttactcgcag tactcagcac tcagtgttct
      361 ttcgacgcat ggacgggcgc tccggacggg tacgcattgg tgagttcaac aacctgttcg
      421 aacatattga ccaacaggag taccgcaaaa ttctggcggg cactgagcag gaaatgcgcc
      481 tgaaaaaatt acgcgcaatg caacggaggt gatacatgca tacggctttt gagttctggg
      541 ttcgcaagac attcggcaat cgctacgacc tgacccgtga tgtcgacggc ttctactgcc
      601 gtgaagttgt gaaacgaatg tttgacgtgt ggtgccactg ccgtggatga aagttttatg
      661 aggttggcat gcagacaatc atctatcaga taacccccag caaatggtgt acggagagag
      721 tccttattgc atcaacaggg ctaaagcccg gcaccatcga gcgggccaga agaaagtcat
      781 ggatgcaggg aaaagaatac cgccattacg ctgtagaagg tgatcctggg cattacagtg
      841 aatgcctgta caacatcgaa gaaattatgc gatggatcga aaaccagaaa caaccaggtg
      901 ccaaaaatgc aagttccggt taacctgtta atgctcctgg acgtctggga ggtttaatga
      961 gtaacgcatc atacccgaca ggcgttgaaa accatggcgg atcactccgt atatggtttc
     1021 actataatgg caaacgtgtc agagaaaacc tcggtgttcc tgacacagcc aaaaaccgga
     1081 agatcgctgg tgaacttcgc acttccgttt gttttgcaat cagaatgggg agtttcgact
     1141 acgccgcgca gttccctaat tcccctaacc tgaaacactt tggtctggga aaaagagaga
     1201 taaccgttaa ggcactttcg gaaaaatggt tggaccttaa gaaaattgag atttgtgcga
     1261 atgcacttaa ccgttaccag tcagtaatta aaaacatgtt accaatgtta ggtgaaaaaa
     1321 aactggtttc atccataaca aaagaggatt tacttttcgt aaggagagat ttgttgaccg
     1381 gttaccaaaa gctttctaat ggaaagactt cttccataaa agggcgctca gtggtcacgg
     1441 taaactacta tatgacaacc atagctggaa tgtttcaatt tgcaacagat aatggttata
     1501 cctcaggaaa cccatttaac ggtctggctc ccttaaaaaa gtccaaggta aaaccagatc
     1561 ctctcacccg tgacgaattt attcgtttta ttgaggcttg ccgtcatcaa caaacaaaaa
     1621 acctgtggat tctcgctgta tacacgggta ttcgtcacgg ggagttggta tcgctggcat
     1681 gggaagatat agaccttaaa gcaaggacta taaccatccg tagaaattat acaaaacttg
     1741 gcgaattcac tccaccaaaa accgatgcag gcaccggaag gacaattcat ctggttcaac
     1801 cagctattga tgctcttaaa agccaggcgg aaatgaccat gcttggaaag caacattctg
     1861 tagaggtgaa gcagagggaa tatgggagaa ctgctgtgca taaatgcact tttgttttta
     1921 gtcctcaggt aacaaaacag cagcagttgt ccggacctca ctacaaggtt gactccatca
     1981 gggagtcatg gacaagtatc ttaaaacgcg caggtctgag acacagaaaa tcgtaccaat
     2041 ccaggcatac ttatgcatgc tggtcacttg ccgcaggagc taatcctagt tttatcgcaa
     2101 gccagatggg ccacacaaac gcacaaatgg tattcaatgt ttacggagca tggatgaaag
     2161 acaacaatca cgaacagata gaactcctta acaaaagact atctgaaagt gtcccatgta
     2221 tgccccataa gaaagcaggg taaaataaaa acttgcaaaa tcaattagtt tacccttaat
     2281 ccctgtcacg ttacgcgcgt ggcagaggcg ttacggattg ctgaaaccgc aacggacaga
     2341 cggcggtcat cggctgttca acgatgccga tattgaccgg atccgcgaga tcaaacgctg
     2401 gatcgacaac ggcgtgcagg tcagcaaagt taaaatgctg ctcagtaatg aaaatgttga
     2461 tgtgcagaac ggctggcgcg atcagcaaga aacattactg acctacctgc aaagcggcaa
     2521 tctgcatagc ctgcgaacgt ggatcaaaga gcgcggtcag gattaccccg cccagacgct
     2581 caccacacat ctgtttattc ctctgcgccg acggcttcag taccaacaac cgactctcca
     2641 ggcgctgctg gcgatcctcg acggcgtact gatcaactac atcgccattt gtctggcttc
     2701 ggcacgtaaa aaacagggta aagatgcgct ggtggttggc tggaatattc aggataccac
     2761 ccgtctgtgg ctggagggct ggattgccag tcaacaagga tggcgcattg atgtcctcgc
     2821 ccactcgctc aatcaactac gccctgaact attcgaaggc cgtacattgc tggtgtggtg
     2881 cggtgaaaat cgaacctccg cccaacagca gcaactcacc agttggcaag aacaaggcca
     2941 tgatattttc ccactcggca tttaatgatt cgttaacaaa tgcgctttac tgtacaatcc
     3001 tttcgttaac ataaggagtg cattatgcgc atagctaaaa ttggggtcat cgccctgttc
     3061 ctgtttatgg cgttaggcgg aattggtggc gtcatgctcg caggttatac ctttattttg
     3121 cgtgctggct aagcgcctgc accagccttt caaacaggcg gtctgcgatg atcgccgcca
     3181 gtgccaccag taacgccccc tggatcacat acgcggtatt aaatccgcta agcccgatga
     3241 tgatgggcgt acccagcgtg ctggccccta ccgttgaggc gatcgtcgcc gtaccaatgt
     3301 tgataatcac cgaggttcgc acgcccgcca gaatcaccgg agccgccagc ggtagctcga
     3361 ccttacgcag tcgctgacct cgactcattc ccataccttt cgcaacttct gtcacgctgg
     3421 catcaatcgc tcccagcccg gcaagtgtcg cctgcaggac gggcagcaca ccataaagga
     3481 tcaaggcgat aatcgctggt tgcagaccaa agcctatcac cggaacagca atcgccagca
     3541 ctgcgacagg cggaaaagtc tgcccaacgg cggcaatagt ttccaccagt gggcgaaatt
     3601 ccgcgcccca cgggcgagtg actgcaattc cggcaccagt gccaatgatc accgcaaaca
     3661 aactcgaaat tcccaccagc cagaaatgag ccagtgccaa agctgcaaaa ctttcttgct
     3721 gataaacggg tcgtggcagt tgtgggaaca aggcagcaaa cagcggctgg ctgtaaggca
     3781 gccagaaaat cagtgccaca aacaaagcaa tgagccagaa cagcggatcg cgcaacatct
     3841 tcatacgctt acgcctccac cagcagatcc tgaaaatgca gcgtgccgct aggctggccc
     3901 tgcgtgttca ccaccggcag cacctcgcat ccccgcgcga caaacaggga gagcgcatcg
     3961 cgcagcgtca tctcttctgc cagtgcctca ccttctgccc gttcttcgcg acgcacgtaa
     4021 tccgccacac tacgtaacga aagcaggcgc acacccagtt cactacgtcc aaaaaactgg
     4081 cggacaaaat cattcgccgg acgagtcagc atcgtcagcg gattcccctg ctgcaccact
     4141 tcaccgtgat ccatcaatac cagatgttct gccagccgta gcgcctcatc aatatcatgc
     4201 gtgaccagca caatggtacg ccccagcaaa cggtgaatgc gcgtcatctc ttgttgcaac
     4261 gcgccgcgcg ttaccgggtc cagtgcgcca aaaggttcat ccatcagtaa gacttgcgga
     4321 tcggcagcca gtgcgcgcgc tactcccaca cgttgctgct gaccaccgga aagctgatgt
     4381 ggataacgct cacgtaaatt tgactccagc cccagtagcg ccattaattc gtcgatacga
     4441 tcgtcaatcc tcgcccgcga ccatttttgt aattgcggca cggtggcaat gttttgtgcc
     4501 acgctccagt ggggaaacag gccaatagat tgaatggcat agcccatacg gcggcgcaac
     4561 tccagtactg gcagcgagcg aatttcttct ccagcaaagc ggagctctcc gctgtcatgt
     4621 tccaccaggc ggttaatcat tttcagggtg gtggatttgc cggagccaga tgtgccaatc
     4681 agcaccgaaa aactcccttc ctgaaaattg agattgagat cgttaacggc tttttgtgcg
     4741 ccgaacagtt tgctgacatg gctaaattca atcattacgt ttcaccttca acagtgcgat
     4801 tagtaaatcg aacagcgcat cgatcagcac cgccagaaca attaccggga tcacccccag
     4861 caacactaaa tcaatggcgc tgcttagcag cccctggaaa accagcgcac caaaaccgcc
     4921 tgcgccgatt aacgccgcaa tcaccgccat gcctacagtt tgcaccatca ccacccgcag
     4981 gctgcgcaga aataccggta acgccagcgg taactgaaca tgcaggaatc gctgcgcccc
     5041 gctcatgccc atcgctctgg cgctctccag cacatcgcgc gggatctggt tcaagccgac
     5101 taccacgccg cgcaccagcg gtagcaaggc atagagcacc agcgcaatca gtgcgggtgt
     5161 cattccggtt cctgctatgc caagcttccc cagccacgga aaggccgtaa ccagtccggc
     5221 aagcggcgca atcagcaggc caaagagcgc cacggaaggc acggtctgaa tgacattgag
     5281 cagagaaaaa atcgccccct gccgcgcggt ggaaaagtag caccagatgc ccaacggcac
     5341 accaatcact aacgcaggca gcaccgcacc aaacagcaac gtcagatgtt gtgccagcgc
     5401 gtcgtcaaac acatcctgac ggttggcgta ttctttcatt agtgagagat cgttaagcgt
     5461 gccggagtac agcaaccaca gcggaataat ggcaatctgc atatgcaaca accagcgcca
     5521 cagcggatgc gtggagattc ggcggatggc atcgctacag gccagcaatg ccagcgccga
     5581 agccaaccag aaaccactgc cgaggctggt acgcgccagc gcactgccat tttgcgccag
     5641 ttgggtcgcc gcctttccag ctccccacac cagcaatacg aagacgaatt gcgccagaat
     5701 gagtgcaaaa atgctgcctt ttttagcggg aataaaacag gctgtcagcc aggcgcaacc
     5761 aacgcccacc agcatccaga tcgtttgcgg ccacagttgc cagagatgac gcccttcacc
     5821 cgagactaaa cgattaggcg cgtagctgat aaacggcagc gctgctgcga ttgccgtcag
     5881 caacagcagc agcgccagaa caggattaat acgtagataa gtcacgggaa attacttcgt
     5941 ccaccctttt tgtttcaagt agtcggcagc cacttttttg gcatccagtc cttccactgc
     6001 aatgctggca ttcagttgct gcaatgtttt tgcatcgagg ctggcgaaga ctggctgtag
     6061 ccactgtgcc atttgcggat actctttcag caccgactca cgcaccactg gtgcaggcgc
     6121 gtagataggt tgcacgcctt gcggatcgct taaggtttgc agcccctgcg ccgcgaccgg
     6181 accgtcagag ccgtaagcca ttgcggcatt aacgccagaa gtttgctggg cagcggcttt
     6241 aatcgttacc gccgtgtcgc cgccagccag tgacagtaac tgatcctgat cgagtttaaa
     6301 gtcgtaggct ttttcaaacg cgggtaacgc atcggcgcgt tcgataaact cagccgaggc
     6361 tgccagtttg aaggtgccgc cctctttcag ataacgcctc aggtcagcaa gcgaagtgag
     6421 tttgtttttc tctgccacat cctgacgcac ggcgatggtc caggtgttat ttgcaggtgc
     6481 gggcgtcagc cagattaact tgttttgctc tgcatcgagt tttttgactt tctcgtaacc
     6541 ttgctgcgcg tttttccacg ctgcatcgtt ttcatcttta aagaaaaacg cgccattgcc
     6601 ggtatattcc ggatagatat ccagttcacc ggaagtaatc gccccgcgca ccacaggagt
     6661 cgttccaagt tgcactttat tgaccgttgg tactccgtgg ctttcgagta cttgcaaaat
     6721 gatattgccg agcagcgcgc cttcggtatc gatttttgaa ccgactttaa cgggggaagc
     6781 cgcgtgcagc ggcaggctca cggctgccaa caaaaccagt gaacctgccc agacctttga
     6841 gagtggcatg atgctttcct cattctttta ctgttgtttt cagcgaatta agagaaaagc
     6901 atagttgata atggcgggat tagcctgagc ggacggattc agttgcagaa tcagataaat
     6961 acttaagagg catattcggt ccggcgtttc ctgccggatg cggcgcgagc gccttaaccg
     7021 gcctacaaag ggcgaaaatt cagcaagtta agatatgaac atcaggcaaa gggcgcaaac
     7081 gtcgcgccct tgctaaagca ttacagcaac tcaaactcgc ctttcttaac gcgtgcggaa
     7141 tcagtgccga taaagacatt gaacttgcca ggctcggcgt catatttcat ctgttgattc
     7201 cagaacttca gcgcctcgat atcaatcggg aagctgacgg tctgggtttc gcccggcttc
     7261 agggtgattt tctcaaagcc tttcagctgc ttcactggac ggctcatgga agccgtcaca
     7321 tcctgcaagt acatctgcac taccgtggcc ccttcgcgtt taccggtgtt cgtcacctgc
     7381 acgctggccg tcactttgcc gtcacgcttc atggtcggcg cagaaagttt cacatcagag
     7441 acggtgaaag tggtatagct cagaccatag ccgaacggat aaagcgcccc gttagcttca
     7501 tcaaaataac gcgaagtgta tttgttcggc ttgtcggcat tatacggacg accggtgttc
     7561 agatggctgt agtacaccgg gatctgcccg acagaacgcg ggaaggacat cggcagctta
     7621 ccggacgggt tgtaatcgcc aaacaacaca tcggcaattg cattaccgcc ttcagtcccg
     7681 gcaaaccagg tttccagaat cgcatcagcc tgctgatctt ctttcaccag cgccagcgga
     7741 cgcccgttca tcagcaccag caccagcggt ttgccggtgg ctttcagggc ggcaatcaaa
     7801 tcacgctggc tttgcagaat agtaatatcg gtacggctgg aggcctcgtg cgccatcccc
     7861 tgtgcttcac cgactacagc caccaccaca tcagattgtt tcgccgtctg caccgcttca
     7921 tcaatcatct cttgcggcga acgcggatcg actttgaccg cttcttcata ctgattcagg
     7981 aaatcgataa tgcctttgtc actggtaacg ttcgcccctt tggcatacag cactttaccg
     8041 ttttcaccga cggcattttt aatcccggtc agcacggtca cggattgatc ggcaacacct
     8101 gccgccgacc agctgcccat cacgtcacgt ttactgtccg ccagtggccc aaccaccgca
     8161 atggtggctg attttttcag cggtaacgtt tcgagacggt ttttcagcaa caccaggctt
     8221 tcacgcgcca cttcacgcgc ttctttgcgg tgcaggcggc tttcggcatt ggtatccacc
     8281 gggtcagact ctttcggacc gagatggctg tatgggtcgt taaacaaccc catatcatat
     8341 ttaacgttca gtacatggcg ggcagcatcg tccagctctg ccatcgtcac tttaccggat
     8401 ttgatcaacc caggcagata cttcgagtag tactcgtcgc tcatgctcat gttgattccg
     8461 gatttcagcg ccacgcgcac tgcatcttcc ggatctgccg ccgtgccatg tttaatcagc
     8521 tctttgattg caccgtgatc ggaaacggtg atgcctttaa agccccactg gtcgcgcaga
     8581 acatctttca gcagccagga gtcggaggtg gctggcgtgc cgttcagcga gttcagcgcc
     8641 accatcaccg cgccgctgcc tgcgtccagc cccgctttgt acggcggcat ataatcatta
     8701 aacaggcgct gcggactcat atcgacggtg ttgtactctt taccgccttc taccgcgccg
     8761 tatgcggcaa agtgtttgac gctggtcatc accgagtaac gatctgccgg gcttttaccc
     8821 tgcatcgctt ccaccatggt tttacccatt attgaggtga gatacgtatc ttcgccaaaa
     8881 ccttcggaag cacgtcccca gcgcggatcg cgcgagacat cgaccatcgg tgcccaggtc
     8941 atattcaggc catcatctgc cgcttcataa gcagagacac gtccgaccgt ttttactgca
     9001 tcgaggttaa aagacgaggc cagaccgagg ctaatcggga acaccgtgcg ctgaccgtgc
     9061 agcacgtcgt aagcaaagaa aagaggaatt ttcaggcggc ttaattccat cacctgatcc
     9121 tgcatggcgc ggatatcctg acgggttacg gtgttgaaaa tagccccaac ctgaccgtct
     9181 ttgatcatct cgcggatcgc ctctttcggg ttatccgggc cgacgctgat taagcgcagc
     9241 tgaccaattt tctcatcaac tgtcattttc ttaagcagtt cggtgacgaa cgcatcccgc
     9301 gcttcaggcg ttaatggatg gttaccgaat aaatcatccg ccagtgctgg ctgcagggcc
     9361 agactcaccg cgattcctac tgaacatagc catttcatat ggatttttct tcctttgttg
     9421 cccgacgtgg cagcgaaaat ggtgcaaaaa ccgtagtttg ccataagcat gatggagaga
     9481 gaaaaagaat gctcagttta ttgtctgaat tttcaaaata ttcactcgct gaattgtcat
     9541 acaaggcgct attctagttt gtgatgtttt ttcgccacca caaggagtgg aaaatgtctt
     9601 ccatgacaac aactgataat aaagcctttt tgaatgaact tgcccgtctg gtcggtcatt
     9661 cacacctgct caccgatccc gcaaaaacgg cccgctatcg caagggcttc cgttctggtc
     9721 atggcgacgc gctggctgtc gttttccctg gctcactact ggaattgtgg cgggtgctga
     9781 aagcctgcgt caccgccgac aaaattattc tgatgcaggc tgccaataca ggcctgaccg
     9841 aaggatcgac gccaaacggt aacgactatg atcgcgatat cgtgatcatc agcaccctac
     9901 gtctcgacaa gctgcacgtt ctcggcaagg gcgaacaggt gctggcctat ccgggcacca
     9961 cgctttattc cctggaaaaa gccctcaaac cgctgggacg cgaaccgcac tcggtgattg
    10021 gttcatcgtg cattggcgca tcggttatcg gcggtatctg taataactct ggtggttcgc
    10081 tggtgcagcg tggcccggcg tataccgaaa tgtcgttatt cgcgcgtata aatgaagatg
    10141 gcaaactgac gctggtgaac catctgggga ttgatctggg cgaaacgccg gagcagatcc
    10201 ttagcaagct ggatgacgat cgcatcaaag atgacgatgt gcgtcacgat ggtcgtcacg
    10261 cccacgatta tgactatgtc caccgcgttc gtgatattga agccgacacg cccgcacgtt
    10321 ataacgccga tccggatcgg ttatttgaat cttctggttg cgccgggaag ctggccgtct
    10381 ttgcagtacg tcttgatacc ttcgaagcgg aaaaaaatca gcaggtgttt tatatcggca
    10441 ccaaccagcc ggaagtgctg accgaaatcc gccgtcatat tctggctaac ttcgaaaatc
    10501 tgccggttgc cggggaatat atgcaccggg atatctacga tattgcggaa aaatacggca
    10561 aagacacctt cctgatgatt gataagttag gcaccgacaa gatgccgttc ttctttaatc
    10621 tcaagggacg caccgatgcg atgctggaga aagtgaaatt cttccgtccg cattttactg
    10681 accgtgcgat gcaaaaattc ggtcacctgt tccccagcca tttaccgccg cgcatgaaaa
    10741 actggcgcga taaatacgag catcatctgc tgttaaaaat ggcgggcgat ggcgtgggcg
    10801 aagccaaatc gtggctggtg gattatttca aacaggccga aggcgatttc tttgtctgta
    10861 cgccggagga aggcagcaaa gcgtttttac accgtttcgc cgctgcgggc gcagcaattc
    10921 gttatcaggc ggtgcattcc gatgaagtcg aagacattct ggcgctggat atcgctctgc
    10981 ggcgtaacga caccgagtgg tatgagcatt taccgccgga gatcgacagc cagctggtgc
    11041 acaagctcta ttacggccat tttatgtgct atgtcttcca tcaggattac atcgttaaaa
    11101 aaggcgtgga tgtgcatgcg ttgaaagaac agatgctgga gctgctacag cagcgcggcg
    11161 cgcaataccc tgccgaacat aacgtcggtc atttgtataa agcaccggag acattgcaga
    11221 agttttatcg cgagaacgat ccgaccaaca gtatgaatcc ggggatcggt aaaaccagta
    11281 agcggaaaaa ctggcaggaa gtggagtaaa aattacggat ggcagagtat cgccatccga
    11341 attcacttaa tcgttctgtg ccgtctgccc cgccgccgcc atttgggcgg ctttttgttt
    11401 tttatagctt aacgctgctg ccggaacagg catcacttta ccggtttcaa tccaggtacg
    11461 caggcggctg gcatcggcaa aatgggtgta tttgccaaac gcatccatca ctaccagcgc
    11521 caccggttta ttattgataa ccgtacgcat caccagacaa tggcccgccg cattggtaaa
    11581 gccggttttg gttagctgga tattccagtt atcgcgatac accagatgat tagtattgcg
    11641 gaacggcagc gtatacgtcg ggttagagaa ggttgccata tcttcccggg tagtacttaa
    11701 ctgcccgatc aacggatatt gtttgctggc aatgagcagt ttggttaagt cacgggcggt
    11761 tgaaacgtta tgcaccgaca atccggtagg ttcaacaaag cgcgtgttgt tcattccgag
    11821 cgatttcgct ttcgcattca ttgccttaat aaaggctttg taaccaccgg gataatggtg
    11881 cgcaaggctt gccgccgcgc ggttttctga agacatcagc gccagcaaca gcatatcttt
    11941 acggctgatt tcgctattca gtcgtacgcg cgaatagacc cctttcatct ccggcgtctg
    12001 gctgatatcc acttttagtt tttcatccag cggcagtcgt gcatccagca cgaccatcgc
    12061 agtcattaat ttgctgatag acgcaatcgg acgcaccaga tccgggtggt tcgaatagat
    12121 cactttgttg gtattcagat caacaatcat cgcgctaccg gaggcaattt ccggttgtga
    12181 agcggtggta gcggctgccg ttttcgcaac ggcctgcggt gccaaaggca cagccagcat
    12241 cagggccagg ctaaataaag aaactcgaaa tttcggcatg atgagcattc agatagtggt
    12301 tcacgcgcac gggttgcgca ccgccggagt aaggatttac tgaggctagc gacgccatca
    12361 taacgagcaa aaagtgcgat cgtcaaagga gaatcgtgag gaaatgctgc attgctgaca
    12421 tttacgccag caatgcaacg tcaaagaact ttctagaaca aacgataacc gtagccccat
    12481 agtataacgg taagggcgag cagcacttcc agtaccagca cgccaatcgc cagcgtcgaa
    12541 ctggagaagc taaggccttc ctctttgttg atattcagga agctcggaat accaaggtaa
    12601 agtaggtaac cggtgtaaaa cagtgccacc gtgccgacca gcgcacacaa ccagaccagc
    12661 ggataaagcg ccaccagacc acttaaaaac agcggagttg caacgtagcc cgcgaagacc
    12721 atgcagtgcg caagtgacgg acgctgcgga taattacgcg ccatccacca gatgacccgc
    12781 cccatcaccg cgaccccagc gagcataacg ccataaaaca ggacagccag cgccagtccg
    12841 gtaaaccagg ataacttcag gatagtgcca tcgccaaaat tccagccaat ctgtgtagtg
    12901 ccaatgaagg cgcaaatcac cggaatcgcc gccatcagca aaacgtggtg ggtgtaatgg
    12961 tgagaaatcg tttcgttttc gcgattaatc acctgcattt cacgatcggg atgggaaaac
    13021 agtccccaga catggctcat accgccccct tgttgtgagt tcatgaacct gacagttcaa
    13081 gtataagtca gcttgtgatt attttttgtt cgcccatgta atttgcaccg tctgatgtac
    13141 gtcctttttc agcgtgaatg attcacaggg tgtatgctta caggcaacca aagggagaca
    13201 gactggccta tggatctcaa tacactcatc tcacaatatg gttatgccgc gctggttatc
    13261 ggtagcctgg cggaaggtga aaccgtgact ttgctgggag gccttgcggc acatcagggg
    13321 ctattaaagt tcccgctggt ggtactttct gtagcgcttg gcggcatgat tggcgaccag
    13381 gtgctctatc tgtgcgggcg gcggtttggc ggtaagctgt tacgccgttt ctctaaacat
    13441 caggataaaa ttgagcgggc gcagaaactg atccaacgcc atccgtatct gtttgtcatt
    13501 ggtacgcgct ttatgtatgg ctttcgggtg attggcccga cgctgattgg tgccagccag
    13561 ctgccgccga aaatctttct gccgcttaat attctcggcg catttgcctg ggcgttgatt
    13621 tttaccacta tcggttacgc tggtggtcag gtgattgcgc cgtggttgca caatctcgac
    13681 cagcatttga agcactgggt ctggttgatt ctggtcgtgg ttctggtggt aggcgtgcgc
    13741 tggtggctga aacgacgcgg gaagaaaaag ccggataatc aggcgtaaaa ccattgccct
    13801 ggataaggcg tgcgcgccgc atccgacatc cggttcacca tgcctgatgc ggcgcttgtg
    13861 cgtcttatca tgcctaccaa aattttaccc attgaaatat ccacacacct cactgcgcta
    13921 ttctgggttg aactgcggat tcgccaacat aaagccgcca tccactatca acgactgccc
    13981 ggtggtgtaa tttgcgccct ccgaacaaag ccacgccacc aggctggcaa tctcatgcgt
    14041 tgcgccaaaa cgccgcagag gaatcgaagg ctccgcgtcg ggcttcacgt cgctatcatc
    14101 catgccgttc attggcgtag cgatcgcccc aggtgcgact gcgttcacca aaatcttatg
    14161 cctgaccaac tccagcgcca ttgctttggt taacccgccg agcgcatgtt tagcggctgt
    14221 gtaggcgctg gcatccggca gcggcgtatg ttcatgtacc gacgtaatgt tgatgatgcg
    14281 accaccctgt ccttgtttca ccatctggcg ggcagcaatt tgcgagcata aaaatgcacc
    14341 atcgacatca acggtaaaaa tcttgcgcca ctcatcgaaa gccatatcaa gaaacggcac
    14401 tttggtcatt gcgcccgcat tattcaccag cacatcaata cgccccagcc gttgaataag
    14461 tttctccagc gcctgcgcac cttctggcag attgccgaga tccagttgca cgatctccgc
    14521 acgtacgccg tggctaacta cctcacgcgc ggtatctttt gccccttctt catctgagtg
    14581 ccaggtaatg ccaatatcaa acccttgctg cgccagtaat aacgcgcact ctttgccgat
    14641 ccccgaatcg gaggcggtaa taatcgctac ctgtgccatc gagttctcca cttaacgctg
    14701 aataaacgtt aagtatagaa gtcacatatc atcagcgttt gtaacccccg cccaacgcac
    14761 cagtgagttg aatggaggca tccagccact gcccttgcaa taacaggcca ttggcccgct
    14821 cacgcagcgc ggggattctg gcttcgctga cgcgggaacc agcaatgatg cccgcgttaa
    14881 agcgggcctg cgcaagacct accacacgca aggcatcgcg ctcaatttgc gcctgatgct
    14941 ggtttttctc cgccagtgtc tgaacctgac tggctgcccg cgccacgtca ttcaccgctt
    15001 caaccaccgc tttgttgtag ctggcgatag acaagttgct ttcggctttt gcgatatcga
    15061 gattggcgtt aagacgacca ctatcgaaaa tgggtagcgt caggcctgcc gtaacgccca
    15121 tttgctgcgc ggaatgacgg aacagatcgc ttaagtgcaa cgcatcctgt tgcaggaagg
    15181 ccatcaggtt gatgtcagga taaaatgccg cttttgccgc atcaatggtg cttagcgatg
    15241 actcaacgta ccagtgcgcc gcctgcaaat ctgcccgccg ggccagtaag gagtacccca
    15301 gttcatcagg aagctggctt gccactttcg gcaacgccac cggtttaagc gtcaatgact
    15361 tagtctggtg atttgtgagt gcaattaacc gtgcctcaat aattttcatt ttccccgcga
    15421 catcgttgag ctgctgccgg gttttgctgg cattaatatc ggtttccaca ccttcaactg
    15481 aagaagtaat cccgttctga tatagctggc gatcggtcgc gataatggtg ttctgctctt
    15541 tttcgatttg ctgcaagacc gtgtttaacg ccgcctgggt ttgccactcc cagtacaggc
    15601 gggctacgct gccagccagc aattggcggg tttgctcgcg ttccgccgcc cgtgctttaa
    15661 ccgtacccag gcgggcagta acctccgccc gattctttcc ccagatatcg agatgccagc
    15721 ccgccgttaa gccaaaagtt ccgttggtgt accacggacc ggtcgtacct gcggccggat
    15781 cgttcagagc aaacggcccc attagccctt ctgccgacat tttttgccgc tccatatccg
    15841 cagaaaagtc gatctgcgga ccatcctgag tagcaactgc cttcgcctgg gcttcagcta
    15901 actgaatgcg ctgttcagcc acctgcatat ccggtgcgtt ctgtagtgca ttgttaatta
    15961 aggaagtgag ttgattatcg tgatactcca gccaccattg gctgtctggc caaccatttt
    16021 tcagcgccgt gggtaatgcg gtgtcaactt gtgcagcggg cgtttgctgg cttaacgcct
    16081 ggcgggtttc atgcataggc gcacacccgg ccagcatcag taacagcgga aaacaggcga
    16141 tggctggata aaaggaatca cgattcatgg gggaataatc aggtaagaaa aggtgcgcgg
    16201 agattaccgt gtgttgcgat atatttttta gtttcgcgtg gcaatacatc agtagcaata
    16261 aaacgacata tccagaaaaa tatacactaa gtgaatgata tcttccgatt tatcttaatc
    16321 gtttatggat aacggcaaag ggcttcgttt tttcctatac ttattcagca ctcacaaata
    16381 aaggaacgcc aatgaaaatt atactctggg ctgtattgat tattttcctg attgggctac
    16441 tggtggtgac tggcgtattt aagatgatat tttaaaaatc agatatgaca tataagttgt
    16501 caggtcagga actagatatt ttgtgtactt gatttgcaaa cagctccggt gttggtggcg
    16561 tcaaaatcgc tgaagcgttc cccgaaggcc ggggcagccc acatggatgt gggctgaggg
    16621 cgcgtcttac aaggacgttt
//
LOCUS       DAZFAB010000099        16626 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZFAB010000099 DAZFAB010000000
VERSION     DAZFAB010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16626
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.099"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..1908)
                     /locus_tag="ACHJWA_003681"
     CDS             complement(<1..1908)
                     /locus_tag="ACHJWA_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6538 domain-containing protein"
                     /protein_id="HFO4310201.1"
                     /translation="MSQRYKLYRRTSGIYVVRISVPQRFRRYAGQCEIHTSTGTHDLH
                     EAKQKSALLLAVWYQTLQEYEQLDYRTLSDCAPLLAGEGMISLSNFAQSIELPISQLI
                     REVVNRNLPVFWLATGQFGFYVDEFNAVEREPGAKREKQSDDEKDQPKEVIILNSAFE
                     LGIESFANGYLRPFNPRHTLDCLLSAGVSEGEAAFRTSGDNQSGGWFFDLPGVDITAD
                     SLLISKVHAEGLRLTWLVKTTPPAVSIHPAVPLVAPVIANEYVHRKHYNENLSWLREE
                     YLKHRRKGKVSEAALRDIRYYFDLMIEVMGDIQLEDFDRDFLRAYESKLRTIPANRNL
                     MKGKHGVKTLDELIAKAAECGDKLMTEESVKKYINGLYGAMEWAVDDGKFLKSPCDNF
                     FPPDDKGEREQDHTDIFEPHEIKAIFSQPWFVAGTVERNAQGRFHQYCPFHYWAPLLG
                     LMTGARVNEIAQLMLDDVLADDGVYYLNLESDSENGKKLKNANSRRKIPVHSTLIELG
                     FIEYVDALKAAGYDRLFPELKPHKTKGYGRPVSAWFNESLLAGRLKLERDRSKSFHSF
                     RHSVSTLLKEKGVSSELRGQLLGHVRGKTETEVRYSKDLKPVHMVEVVEKIDFSLPEI
                     ARFNIPDGLDAV"
     gene            complement(1999..2083)
                     /locus_tag="ACHJWA_003682"
     tRNA            complement(1999..2083)
                     /locus_tag="ACHJWA_003682"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2047..2049),aa:Leu,seq:caa)
     gene            2279..3298
                     /gene="ahr"
                     /locus_tag="ACHJWA_003683"
     CDS             2279..3298
                     /gene="ahr"
                     /locus_tag="ACHJWA_003683"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFO4310202.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELRPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLPVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIKHVRDGKARYRVV
                     LKADF"
     gene            3428..4930
                     /gene="yjgR"
                     /locus_tag="ACHJWA_003684"
     CDS             3428..4930
                     /gene="yjgR"
                     /locus_tag="ACHJWA_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFO4310203.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIDVNDWQPHANPVVVWDIFG
                     EKGHSVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPAKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(5091..6173)
                     /gene="lptG"
                     /locus_tag="ACHJWA_003685"
     CDS             complement(5091..6173)
                     /gene="lptG"
                     /locus_tag="ACHJWA_003685"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFO4310204.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(6173..7273)
                     /gene="lptF"
                     /locus_tag="ACHJWA_003686"
     CDS             complement(6173..7273)
                     /gene="lptF"
                     /locus_tag="ACHJWA_003686"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFO4310205.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(7336..7386)
                     /gene="ytgA"
                     /locus_tag="ACHJWA_003687"
     CDS             complement(7336..7386)
                     /gene="ytgA"
                     /locus_tag="ACHJWA_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFO4310206.1"
                     /translation="MSIIILATISTTQENK"
     gene            7540..9051
                     /gene="pepA"
                     /locus_tag="ACHJWA_003688"
     CDS             7540..9051
                     /gene="pepA"
                     /locus_tag="ACHJWA_003688"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFO4310207.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            9405..9848
                     /gene="holC"
                     /locus_tag="ACHJWA_003689"
     CDS             9405..9848
                     /gene="holC"
                     /locus_tag="ACHJWA_003689"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFO4310208.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            9848..12703
                     /gene="valS"
                     /locus_tag="ACHJWA_003690"
     CDS             9848..12703
                     /gene="valS"
                     /locus_tag="ACHJWA_003690"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFO4310209.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(12758..13954)
                     /gene="yjgN"
                     /locus_tag="ACHJWA_003691"
     CDS             complement(12758..13954)
                     /gene="yjgN"
                     /locus_tag="ACHJWA_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFO4310210.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFFSCLVFVFFYFAILMTVSADMPLIGCV
                     LTLSLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGVWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIVSVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLPFLAVAGYIIFDQILNAYDSSVYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGIM
                     PSLPFL"
     gene            14147..14650
                     /gene="yjgM"
                     /locus_tag="ACHJWA_003692"
     CDS             14147..14650
                     /gene="yjgM"
                     /locus_tag="ACHJWA_003692"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO4310211.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLAGSESDICELQKMYFLPAIRGK
                     GLAKKLALKAMEEAREMGFKRCYLETTAFLKEAIGLYEHLGFQHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(14696..15112)
                     /gene="rraB"
                     /locus_tag="ACHJWA_003693"
     CDS             complement(14696..15112)
                     /gene="rraB"
                     /locus_tag="ACHJWA_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFO4310212.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            15274..16278
                     /gene="argF"
                     /locus_tag="ACHJWA_003694"
     CDS             15274..16278
                     /gene="argF"
                     /locus_tag="ACHJWA_003694"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFO4310213.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKPFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            complement(16335..>16626)
                     /locus_tag="ACHJWA_003695"
     CDS             complement(16335..>16626)
                     /locus_tag="ACHJWA_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310214.1"
                     /translation="LLKSYEDEKIYFDKLGYNFNNKESNEEIMKNQPKDVIEEKLNNE
                     LKLRFRMMQTILKSEVNVSPFIDQQRLNTLNPPENLRIAIEKFGWKKKTITA"
BASE COUNT         4312 a         4181 c         4225 g         3908 t
ORIGIN      
        1 tacagcatcc agcccatcag gaatgttgaa tctcgctatc tcgggcaaag aaaaatcaat
       61 cttttcgaca acctcaacca tgtgaaccgg ttttaaatct ttgctgtatc gcacttcagt
      121 ttctgttttg cctcgcacat gcccaagtag ctgcccacgc agttccgaac taacaccctt
      181 ctctttaagc aaagttgaaa cagaatgccg gaaagagtgg aaagatttgc ttctgtctct
      241 ttcaagtttt aatcgacccg caagcaatga ttcattgaac catgcggaaa ccggcctacc
      301 atagcctttg gttttatgtg gtttaagctc gggaaaaaga cggtcatacc cggcagcttt
      361 caacgcatcc acatactcga taaaaccgag ttcaatcagc gtagaatgaa ccggaatctt
      421 gcggcgggaa ttggcgtttt ttagtttctt tccgttttcg ctatcgcttt caaggttcag
      481 gtaataaacg ccgtcatctg ccagaacatc gtccagcatt aactgtgcaa tctcgttaac
      541 ccttgccccc gtcatcaagc ccaacaacgg cgcccaatag tgaaacgggc aatattgatg
      601 gaatcgccct tgcgcattac gttcaacagt tccagcgaca aaccacggtt gcgaaaaaat
      661 tgccttaatt tcatgcggtt caaatatgtc agtgtgatcc tgctctcgct cacctttgtc
      721 atcgggaggg aaaaagttgt cgcatggcga tttcagaaac ttaccatcat caacagccca
      781 ctccattgca ccataaaggc cgttgatata ctttttgaca gactcttctg tcatcagttt
      841 atcgccacat tctgccgctt tggcgattaa ctcatccagc gtcttaaccc cgtgcttacc
      901 tttcatcaaa ttacggttag caggaattgt gcgcaacttg ctctcataag cccggaggaa
      961 atcacggtcg aaatcttcca actgaatatc ccccatcact tcaatcatca aatcgaagta
     1021 atagcgaata tcgcggagcg ccgcttctga taccttgccc ttacgtcgat gtttcaaata
     1081 ctcttcacga agccatgaca agttttcatt gtaatgtttg cggtgaacat attcattagc
     1141 gataacagga gcgacaagag gcacggcagg gtgaatgcta actgctggtg gcgtggtctt
     1201 aaccagccat gtaagtcgaa ggccttcagc atgaactttg ctaatcaaca ggctatcagc
     1261 agttatatct acgccgggta aatcgaagaa ccaacctcca ctttggttat caccactagt
     1321 tcgaaatgca gcctctcctt cggatactcc agcgctcaac agacaatcta aagtatgccg
     1381 gggattgaag gggcggagat aaccatttgc gaagctctcg atacccagct caaacgcgct
     1441 attgagaatg atgacttctt taggttgatc cttttcatca tcagactgtt tttctcgttt
     1501 tgcacccggt tcccgctcta ctgcattaaa ttcatcaaca tagaaaccga actgaccagt
     1561 cgccagccag aataccggga ggttacgatt aaccacctct cgaatcaatt gcgatatagg
     1621 caactcgatt gactgagcaa agttagaaag cgagatcata ccctcaccag caagcagtgg
     1681 ggcgcagtca cttaaagttc gataatccaa ttgttcatac tcttgtaagg tctgatacca
     1741 gaccgccaac aggagcgcgg acttctgctt tgcttcatga agatcgtgag tgcctgttga
     1801 agtgtgaatc tcacactgtc ctgcgtatcg acgaaaacgt tggggaacgc taatgcggac
     1861 aacataaatg ccgctggttc gacgatagag tttatatcgc tgagacatcg cctcgtatac
     1921 ccttttgtaa gaggggctac gcgatttcaa gcaagattat gaattaaatt gttttaaaac
     1981 aatagtttag atgagtcttg gtgccgaagg ccggactcga accggcacgt atttctacgg
     2041 ttgattttga atcaactgcg tctaccgatt tcgccacttc ggcactgaag aggtatgcgg
     2101 aaaacgttgt ggattatacc tgtcgcacgc caccatgcaa gcgccagctt gctcgcacct
     2161 cgctaagtgc tgaaattttc agcattgcat acagcgatgt gtaacctttg tcacactcca
     2221 ggcaccccgc cctgccatgc tctacacttc ccaaacaaca ccagagaagg accaaaaaat
     2281 gtcgatgata aaaagctatg ccgcaaaaga agcgggcggc gaactggaag tttatgagta
     2341 cgatcccggt gagctgaggc cacaagatgt tgaagtgcag gtggattatt gcgggatctg
     2401 ccattccgat ctatcgatga tcgacaatga atggggattt tcacaatatc cgctggttgc
     2461 cgggcatgag gtgattgggc gcgtggtggc gctcgggagc gccgcgcagg ataaaggttt
     2521 gcaggtcggc cagcgtgtcg ggattggctg gacggcgcgt agctgtggtc actgcgacgc
     2581 ctgtattagc ggtaatcaga tcaactgcga gcaaggtgct gtgccgacga ttatgaatcg
     2641 cggtggcttt gccgagaagt tgcgtgcgga ctggcaatgg gtgattccac tgccagaaaa
     2701 tattgatatc gagtccgccg ggccgctgtt gtgcggcggt atcacggtct ttaaaccact
     2761 gttgatgcac catatcactg ctaccagccg cgttggggta attggtattg gcgggctggg
     2821 gcatatcgct ataaaacttc tgcacgcaat gggatgcgag gtgacggcct ttagttctaa
     2881 tccggcgaaa gagcaggaag tactggcgat gggtgccgat aaagtggtga atagccgcga
     2941 tccgcaggca ctgaaagccc tgtcggggca gtttgatctc attatcaata ctgtgaacgt
     3001 cagcctcgac tggcagcctt attttgaggc gctgacctat ggcggtaatt tccatacggt
     3061 cggtgcggtt ctcacgccgt tgccggttcc ggcctttacg ctgattgcgg gcgatcgcag
     3121 tgtctctggc tctgctaccg gtacacctta tgagttgcgt aagctgatgc gctttgccgc
     3181 ccgcagcaag gttgcgccga ctaccgaatt gttcccgatg tcgaaaatta acgatgccat
     3241 caagcatgtg cgcgacggta aggcgcgtta ccgcgtggtt ctgaaagctg acttctgatc
     3301 tctttgccgg gtgcctgtcg cccggcactt aaatctcttt ggaaggcgtc tcgcaatctg
     3361 ctgaatttag cggtgaagag tgcgcagccg ttgcctatac tccaggcagg aaactggagg
     3421 atatctcatg agcgaacccc tgttaattgc ccgcacgccg gacacagaac tatttttact
     3481 gccgggaatg gctaaccgtc acgggctgat tactggcgca acggggacgg gtaaaactgt
     3541 cacgctgcaa aaactggcgg agtcattgtc ggaaatcggc gtgccggtgt ttatggctga
     3601 tgtgaaaggc gatctgaccg gtatcgcgca ggcaggaacg gcgtcggaaa aactgctcgc
     3661 aaggcttaaa aatatcgacg tcaatgactg gcaaccgcat gctaatccgg tggtggtgtg
     3721 ggatatcttt ggcgagaaag gccattcggt acgggcgacg gtttcagacc tggggccact
     3781 gttgctggcg cggctgttga atctcaacga tgtgcaatct ggcgtgctga atatcatctt
     3841 ccgcattgct gacgatcagg ggctgttact gctcgacttt aaagatctgc gggcgattac
     3901 ccagtacatc ggcgataacg ccaaatcttt ccagaatcag tacggaaata tcagtagcgc
     3961 atcggttggt gccatccagc gcgggctgtt gtcgctggaa cagcaaggcg cagcacactt
     4021 ctttggtgag ccgatgctgg atatcaaaga ctggatgcgc accgatgcca acggtaaagg
     4081 cgttatcaat atcctcagcg ccgagaaact ttatcagatg ccgaaactgt acgccgccag
     4141 cctgctgtgg atgctctcag agttgtatga acaattgccg gaagcgggcg atctggagaa
     4201 accaaaactg gtgtttttct tcgacgaagc acatctgctg tttaacgatg caccgcaggt
     4261 actgctggat aagattgagc aggtgataag gcttattcgc tcaaaaggcg tgggcgtctg
     4321 gttcgtttcg caaaacccgt ctgatattcc ggataatgtg ctcggccagc tcggtaatcg
     4381 cgttcagcac gctttgcggg cttttacgcc caaagatcag aaagcagtaa aagctgcggc
     4441 gcaaaccatg cgggccaatc cggcgtttga taccgaaaag gcgattcagg aactgggcac
     4501 cggcgaggcg ttgatctctt ttctggatgc gaaaggaagc ccttctgtgg tggagcgtgc
     4561 gatggtgatc gcgccttgtt cgcggatggg gccggtgacg gaagatgagc gtaatggctt
     4621 gattaatcac tctccggtgt atggcaaata tgaggatgag gtggaccggg aatccgccta
     4681 tgagatgttg caaaaaggct ttcaggccag taccgagcag caaaataatc cccccgcgaa
     4741 agggaaagag gtagcggtgg atgacggcat tcttggtgga ttgaaggata ttttgttcgg
     4801 cactaccgga ccacgcggcg ggaagaaaga tggtgtggtg caaacaatgg ccaaaagcgc
     4861 cgctcgccaa gtgacgaatc agattgtacg cgggatgttg gggagtttgc tgggggggag
     4921 aagaaggtaa gtcaggcgag atttgtcttt gcctgatgcg acgctggcgc gtcttatcat
     4981 gcccacccca ctgcaatata ttgaatttta attatttttc aggaatgaac gaagcacact
     5041 ttgctaacaa taataaaggg agctttcgct ccctttattc gttcattcgg ttacgatttt
     5101 ctcattaaca gccacaggct gattaagaag aagctggcgc ttggcaacag tgcgccgatg
     5161 atcggcggga tgccataaac caacgtcagc gggccgaaga tctggtccag tacgtagaag
     5221 acaaaaccga aactgatacc ggtgaccaca cgcacgccca tcggtacgct acgcagtggg
     5281 ccaaagatga acgacagcgc catcagcatc atcaccgcca cggatagcgg ctggaagatt
     5341 ttgctccaca tgttgagctg ataacgtccg gcatcctgac cgctcgactt cagatacttc
     5401 acatagttgt gcaaaccgct gatagagagt gcatccgggt ccagcgccac cacgcccagt
     5461 ttgtccggcg tgaggttggt tttccaggtg ccgctcaccg tctgcgaacc ggtaatctgt
     5521 ttcggattgg tcagatcaga ttcatcaacc tgcgacaaac gccagacttt atgttccggg
     5581 tcaaacttcg cagtagcggc atagcgtacg gattgcagac gacgattctc gttaaaggca
     5641 taaatgctga tgccacctaa ctcttcgtca cctttaaccc gctcaatgta gacgaagttg
     5701 ttgccatctt tcgcccataa gccttgctgg gtagagagca acgagccgcc gtacatcgcc
     5761 tgcgcacggt agttacgcgc catctgctcg ccctgcggcg cgacccattc gccaatcgcc
     5821 atcgtcagca agaccagcgg aatggcggtt ttcatcaccg acagcgccac ctgcatacgg
     5881 gtaaaaccag aagcctgcat caccaccagt tcgctgcgct gcgccagcat cccaagacca
     5941 agcaacgccc caagcagagc cgccatcggg aagaatattt gcacatcttt cggcacgctc
     6001 agcaaggtat acattcctgc gcctaacgcg tcgtaactcc cctgcccggc ttttttcaac
     6061 tgatcgacaa acttgataat gcccgacagc gacaccagca tgaacagcgt catcatgatg
     6121 gtggtgaaaa tagttttacc gatatagcgg tcaagtacgc caaaaggttg catcacaccg
     6181 ctcctttacg cgaaaaactg gcgcgcaggc ggcggaccgg cacggtgtcc caaaggttga
     6241 gaacaatcgc caaagccaga taaatcaggt taacggtcca catccacagc gtcgggtcca
     6301 gcttaccttt accgccgttc gatttcaggg aggtctggat caggaagaaa agtagataca
     6361 gcagcatggc tggcagcatc gacagtacgc gtccctgacg tgggttaacc acgctcagcg
     6421 gtacgaccat aagtgccatc ataaacacgg tgaataccaa cgtgatacgc cagttcagtt
     6481 ctgcgcgagc acgatcggtg tcagtgttcc acaatgtgcg catgtccatc tggtcggtat
     6541 cgttcgggtc gagcgccacc gcctggtgac caatgatcgc ctgataatcc tggaaatccg
     6601 taatgcggaa atcacgtaac agtgcagtgc cttcgaagcg cgttccctgg ttgagagtga
     6661 cgacctggga gccgtcgcgc agctgggtta aatgtccgga atcggccacc accacagaag
     6721 gacgtgcatt accttttggt cgaatttgcg cgaggaacac atctttgaaa tcgctgccgt
     6781 caacgctttc gatgaacagc accgaactgc cattagtcgc ttgctggaat tgcccttgcg
     6841 ccagcgccgc catgccaggg ttcgctttcg cttctgctaa cacttcatcc tgatgacgcg
     6901 atgaccacgg tcccgcccac atcacgttaa ccgccgcgac gattgccgtg aataccgcaa
     6961 ggatcattgc cgctttcacc agaaccgctt tgctcaggcc gcaggcatgc attaccgtaa
     7021 tttcactttc ggtatacagt ttgcccagcg tcatcagcag cccgaggaac aggcttaatg
     7081 gcaggataag ctgcgccatt tccggcacgc ccaacccgag aagggagagc accagattcg
     7141 ccggaatatc gccgtcaacc gctgcgccga ggatcctcac taacttttga cagaagaaga
     7201 tcaaaagcaa gatgaagagt atcgccagct ggcttttgag cgtctcccgc accagatatc
     7261 ttatgattat cactttaaat acgcccgtaa aaactcgtct tttgcaggat tttagcttgt
     7321 ttcatggctt aaacgtcatt tattctcttg agtcgtcgaa atcgtcgcta agataattat
     7381 actcaacgga ttcacctctc agattttgtt ctgacgtgcc aatgccgtaa taacgttaag
     7441 attaacacga agtcatcgca acagcggaca tgagttacga aagcttgcaa ttctatctgt
     7501 agccaccgcc gttgtcttta agattcagga gcgtagtgca tggagtttag tgtaaaaagc
     7561 ggtagcccgg agaaacagcg gagtgcctgc atcgtcgtgg gcgtcttcga accacgtcgc
     7621 ctttctccga ttgcagaaca gctcgataaa atcagcgatg ggtacatcag cgccctgcta
     7681 cgtcggggcg aactggaagg aaaaccgggg cagacattgt tgctgcacca tgttccgaat
     7741 gtactttccg agcgaattct ccttattggt tgcggcaaag aacgtgagct ggatgagcgt
     7801 cagtacaagc aggttattca gaaaaccatt aatacgctga atgatactgg ctcaatggaa
     7861 gcggtctgct ttctgactga actgcacgtt aaaggccgta acaactactg gaaagtgcgt
     7921 caggctgtcg agacggcaaa agagacgctc tacagtttcg atcagctgaa aacgaacaag
     7981 agcgaaccgc gtcgtccgct gcgtaagatg gtgttcaacg tgccgacccg ccgtgaactg
     8041 accagcggtg agcgcgcgat ccagcacggt ctggcgattg ccgccgggat taaagccgcg
     8101 aaagatctcg gtaatatgcc gccgaatatc tgtaacgccg cttacctcgc ttcacaagcg
     8161 cgccagctgg ctgacagcta cagcaagaat gttatcaccc gcgttatcgg cgaacagcag
     8221 atgaaagagc tggggatgca ttcctatctg gcggtcggtc agggttcgca aaacgaatcg
     8281 ctgatgtcgg tgattgagta caaaggcaac gcgtcggaag atgcacgccc aatcgtgctg
     8341 gtgggtaaag gtttaacctt cgactccggc ggtatctcga tcaagccttc agaaggcatg
     8401 gatgagatga agtacgatat gtgcggtgcg gcagcggttt acggcgtgat gcggatggtc
     8461 gcggagctac aactgccgat taacgttatc ggcgtgttgg caggctgcga aaacatgcct
     8521 ggcggacgag cctatcgtcc gggcgatgtg ttaaccacca tgtccggtca aaccgttgaa
     8581 gtgctgaata ccgacgctga aggccgcctg gtactgtgcg acgtgttaac ttacgttgag
     8641 cgttttgagc cggaagcggt gattgacgtg gcgacgctga ccggtgcctg cgtgatcgcg
     8701 ctgggtcatc acattaccgg tctgatggcg aaccataatc cgctggccca tgaactgatt
     8761 gccgcgtctg aacaatccgg tgaccgcgca tggcgcttac cgctgggtga cgagtatcag
     8821 gaacaactgg agtccaattt tgccgatatg gcgaacattg gcggtcgtcc tggtggggcg
     8881 attaccgcag gttgcttcct gtcacgcttt acccgtaagt acaactgggc gcacctggat
     8941 attgcaggaa ccgcctggcg ttctggtaaa gcaaaaggcg caaccggtcg tccggtagcg
     9001 ttgctggcac agttcctgtt gaaccgcgct gggtttaacg gcgaagagta attgcgtcag
     9061 gcaaggctgt tattgccgga tgcggcgtga acgccttatc cgacctacac agcactgaac
     9121 tcgtaggcct gataagacgc gcaagcgtcg catcaggcaa tgctgttatt gtcggatgcg
     9181 gcatgaacgc cttatccgac ctacacagca ccgaactcgt aggcctgata agacgcggca
     9241 gcgtcgcatc aggcaatgct gttattgtcg gatgcggcgt gagcgcctta tccgacctac
     9301 acacagcact gaactcgtag gcctgataag acacaacagc gtcgcatcag gcgctgcggt
     9361 gtatacctga tgcgtattta aatccaccac aagaagcccc atatatgaaa aacgcgacgt
     9421 tctaccttct ggacaatgac accaccgtcg atggcttaag cgccgttgag caactggtgt
     9481 gtgaaattgc cgcagaacgt tggcgcagcg gtaagcgcgt gctcatcgcc tgtgaagatg
     9541 aaaagcaggc ttaccggctg gatgaagccc tgtgggcgcg tccggcagaa agctttgttc
     9601 cgcataattt agcgggagaa ggaccgcgcg gcggtgcgcc ggtggagatc gcctggccgc
     9661 aaaaacgcag cagcagcccg cgcgatatat tgattagtct gcgaacaagc tttgcagatt
     9721 ttgccaccgc tttcacagaa gtggtagact tcgttcctta tgaagattct ctgaaacaac
     9781 tggcgcgcga acgctataaa gcctaccgcg tggctggttt caacctgaat acggcaacct
     9841 ggaaataatg gaaaagacat ataacccaca agatatcgaa cagccgcttt acgagcactg
     9901 ggaaaagcag ggctacttta agcctaatgg cgatgaaagc caggaaagtt tctgcatcat
     9961 gatcccgccg ccgaacgtca ccggcagttt gcatatgggt cacgccttcc agcaaaccat
    10021 catggatacc atgatccgct atcagcgcat gcagggcaaa aacaccctgt ggcaggtcgg
    10081 tactgaccac gccgggatcg ctacccagat ggtcgttgag cgcaagattg ccgcagaaga
    10141 aggtaaaacc cgtcacgact acggccgcga agctttcatc gacaaaatct gggaatggaa
    10201 agcggaatcc ggcggcacga ttacccgtca gatgcgccgt ctcggcaact ccgtcgactg
    10261 ggaacgtgaa cgcttcacga tggacgaagg cctgtccaat gcggtgaaag aagttttcgt
    10321 tcgtctatat aaagaagacc tgatttaccg tggcaaacgc ctggtaaact gggacccgaa
    10381 actgcgcacc gctatctctg acctggaagt ggaaaaccgc gaatcgaaag gttcgatgtg
    10441 gcacatccgc tatccgctgg ctgacggtgc gaaaaccgca gacggtaaag attatctggt
    10501 ggtcgcgact acccgtccgg aaaccctgct gggcgatact ggcgtagccg ttaacccgga
    10561 agatccgcgt tacaaagatc tgattggcaa atatgtcatt ctgccgctgg ttaaccgtcg
    10621 tattccgatc gttggcgacg aacacgccga catggaaaaa ggcaccggct gcgtgaaaat
    10681 cactccggcg cacgacttta acgactatga agtgggtaaa cgtcacgcgc tgccgatgat
    10741 caacatcctg acctttgacg gcgatatccg tgaaagcgcc caggtgttcg ataccaaagg
    10801 taacgaatct gacgtttatt ccagcgaaat cccggcagag ttccagaaac tcgagcgttt
    10861 tgctgcacgt aaagcggtcg ttgctgcggt tgacgcactc ggcctgctgg aagaaattaa
    10921 accgcacgac ctgacagttc cttacggcga ccgtggcggc gtagttatcg aaccaatgct
    10981 gaccgaccag tggtacgtac gtgccgatgt actggcgaag ccggcagttg aagcggttga
    11041 gaacggcgac attcagttcg taccgaagca gtacgaaaac atgtacttct cctggatgcg
    11101 cgatattcag gactggtgta tctctcgtca gctgtggtgg ggtcaccgta tcccggcatg
    11161 gtacgacgaa gcgggtaacg tttatgttgg ccgcaacgaa gaagaagtgc gcaaggaaaa
    11221 taacctcggt gccgacgttg ccctgcgtca ggacgaagac gttctcgaca cctggttctc
    11281 ttctgcgctg tggaccttct ctaccctagg ctggccggaa aacaccgacg cactgcgcca
    11341 gttccaccca accagcgtga tggtatctgg tttcgacatc atcttcttct ggattgcccg
    11401 catgatcatg atgaccatgc acttcatcaa agatgaaaat ggcaaaccgc aggtgccgtt
    11461 ccacaccgtt tacatgaccg gtctgattcg tgacgacgaa ggccagaaga tgtccaaatc
    11521 caagggtaac gttatcgacc cgctggatat ggttgacggt atttcgctgc cagaactgct
    11581 ggaaaaacgt accggcaata tgatgcagcc gcagctggcg gacaaaatcc gtaagcgcac
    11641 cgagaagcag ttcccgaacg gtattgagcc gcacggcacc gatgccctgc gcttcaccct
    11701 ggcggcactg gcgtctaccg gtcgtgacat caactgggat atgaagcgtc tggaaggcta
    11761 ccgtaacttc tgtaacaagc tgtggaacgc cagccgcttt gtgctgatga acacggaagg
    11821 tcaggattgc ggcttcaacg gcggcgaaat gacgctgtcg ctggcggacc gctggattct
    11881 ggcggagttc aaccagacca tcaaagcgta ccgcgaagcg ctggacagct tccgcttcga
    11941 tatcgccgca ggcattctgt atgagtttac ctggaaccag ttctgtgact ggtatctcga
    12001 gctgaccaag ccggtaatga acggtggcac cgaagcagaa ctgcgcggta ctcgccatac
    12061 gctggtgact gtactggaag gtctgctgcg cctcgcgcat ccgatcattc cgttcatcac
    12121 cgaaaccatc tggcagcgtg tgaaagtact ttgcggtatc actgccgaca ccatcatgct
    12181 gcagccgttc ccgcagtacg atgcatctca ggttgatgaa gccgcactgg ccgacaccga
    12241 atggctgaaa caggcgatcg ttgcggtacg taacatccgt gcagaaatga acatcgcgcc
    12301 gggcaaaccg ctggagctgc tgctgcgtgg ttgcagcgcg gatgcagaac gtcgcgtaaa
    12361 tgaaaaccgt ggcttcctgc aaaccctggc gcgtctggaa agcatcaccg tgctgcctgc
    12421 cgatgacaaa ggtccggttt ccgttacgaa gatcatcgac ggcgcagagc tgctgatccc
    12481 gatggctggc ctcatcaaca aagaagatga gctggcgcgt ctggcgaaag aagtggcgaa
    12541 gatcgaaggc gaaatcagcc gtatcgagaa caagctggcg aatgaaggct ttgtcgcccg
    12601 cgcaccggaa gcggtcatcg cgaaagagcg tgagaagctg gaaggctatg cggaagcgaa
    12661 agcgaagctg attgaacagc aggctgttat cgccgcgctg taatcaaaac acagtgatga
    12721 aaacgaaggc cggagcatgc tccggccttt ttatctctta cagaaatggt aaagaaggca
    12781 taattccccg tgaaatactg gctaagaagc ctttatctgg ttgttcttct ttattgatta
    12841 aaggaaggtc atccaaatcg cccagcaaat acgtattttt tgcctgccag tcaatcatcc
    12901 atattttcag cagtggataa gccagaccgc ccgtaatccc ggatatcacc accaacgcac
    12961 acatgcgata aagcataccg tggtatgtta aagttgagcg aaaacgaata cgcccatcat
    13021 tcagtgacag gttgctcata aaatgatttc gcaacgacac cgttaaataa ctggtgctaa
    13081 cagcaatccc aaaataatag attaactgcg cgattatcat tttacgttgc atttccataa
    13141 attgctgtaa attctcaata tcatcatttg catacacaga actatcatac gcatttaata
    13201 tttgatcgaa gataatataa ccagcaacag cgagaaaagg taataaagct aaaaatgcga
    13261 ggatggcata ttttatacaa tacgcagtat ctaatttcac cttgaaacga tgtataccga
    13321 aactggtatt gctccagaga aaactcatta ccaggctata taaagtacca ttaaaaatac
    13381 caatggaaac aataccaact attgccatca gaaccacaga tacaataaca ctacttgaac
    13441 tattggcatg tagcatcttt gtcgagataa agaaaacagt ccccatccca atggccatta
    13501 aaatcggcaa gaaaaaggtt acccaccaga cccctttcat agagcaatta aaactaaatc
    13561 ttacgccgtt gagactggtc atcaaggcct gataacgcag tccttttgct gccataaata
    13621 taagcaaaac caacagtgac aaagtcaaaa cacagccaat aagcggcata tctgctgaca
    13681 ctgtcattaa gattgcgaaa tagaaaaaaa caaaaacaag acaactaaaa aaaacattcc
    13741 caccagtaat tccataagaa aatcgttgtc cgttaacttc catattagca tagagataac
    13801 gcttacattt cattaatgcc catggtaaat agatacctag cgttataatc gttaacaaca
    13861 cattcaccac acaaataaga aaatatctct cacctgtacc atgaaaaaca aacgaatggg
    13921 acggaacatc catttcatta ataacttgag ccataaaaat tccctgaatt aaaaaatcca
    13981 ttgatataat ttatagatac agacagtaaa atgttctgcc acgttacaat actcttatca
    14041 gtaataggct ggcaaaaact ttattgcttt ataagacgct tgcgcacgta agaagagagt
    14101 ggtattaaac aaatgtataa ttactttatt tttattgggt taaactatga ataacattgc
    14161 gccgcaatca cctgtaatgc gtcgcctgac gctgcaggat aatcctgcta tcgcccgcgt
    14221 cattcgtcag gtatccgccg aatacggtct taccgctgat aaaggctaca ccgtcgccga
    14281 tccgaatctt gacgagctgt atcaagtgta tagtcagccg ggtcatgcat attgggtggt
    14341 tgagtacgag ggtgaagtgg tcggcggcgg tgggattgcg ccattagcag gtagtgaatc
    14401 ggacatttgc gaactgcaaa agatgtattt tcttccggct attcgcggca aagggctggc
    14461 aaaaaaactg gccttaaagg cgatggagga ggcaagagaa atgggcttca aacgctgcta
    14521 tctggaaacg accgcttttt tgaaagaagc gattggtttg tatgaacatt taggttttca
    14581 acatatcgac tatgcacttg gctgcacggg ccatgttgat tgtgaagtgc ggatgctacg
    14641 cgaactctaa aaaaatgccc gctagtgtgt gctatcgggc attttaatca attaattagt
    14701 ggcgaacccc gtcatcgtct tcatcgacaa aatcttcatc gtcgccgtct tcgccgttgg
    14761 gatcttcaaa gtaagtgccc caaccgtcgt actcaacgtc aaatttctcc gccagcgtca
    14821 tcagttgttc aacctgggca tcgatcagat cggcattcaa cgcgcactcg ctgaggatgt
    14881 cgcagcaaat cacgatatca ccatcttcaa cttccagctc ttctggatcg gtcacttcgt
    14941 aaccgagttt aaacgcttca actgctgctt tttccagggt ttccagatcg tctgcggaaa
    15001 gatggtgttc gatggtgtac agcgcgtccg gatcgctgcc atcttccagt aattcttcaa
    15061 taatcaaacg tgtttcttca cgctgttctt ccagttgttc cgggtttgcc atggctcatt
    15121 cctcttaatg tcctgccgat actcatattg tcacacacca ctggcattgc ctccaccttt
    15181 gtgacaaaga tttatgcttt agacttgcaa atgaataatc atccatataa attgaatttt
    15241 aattcattga gacgttagcc acaggaggga tctatgtccg ggttttatca taagcatttc
    15301 ctgaaattac tcgatttcac gccagctgaa ctcaacagcc tgctgcagtt agccgcgaag
    15361 ctgaaagccg ataagaaaag cggcaaagaa gaagccaaac tcactggtaa aaacatcgcg
    15421 ctcatcttcg aaaaagactc gacccgtacc cgatgctctt tcgaagttgc cgcatatgac
    15481 cagggtgctc gcgttactta tctcggccca agcggcagtc agattggtca taaagagtcg
    15541 attaaagaca ctgcccgcgt gctgggtcgc atgtatgacg gtattcagta tcgcggctat
    15601 ggtcaggaga ttgtcgaaac actggcggaa tacgctggcg tgccggtatg gaacggcctg
    15661 accaatgagt ttcaccccac gcagttgctg gcggatctcc tcaccatgca ggagcattta
    15721 cccggtaaac cattcaacga aatgacgctg gtctatgcag gtgacgcacg taacaacatg
    15781 ggcaattcga tgctcgaagc tgcggcgctt accggtctgg atttgcgtct ggtcgcgcca
    15841 caggcatgct ggccggaagc tgcgctggtt acggaatgcc gcgccctggc acaacaaaat
    15901 ggcgggaata ttacgctgac ggaagatgta gctaaaggcg ttgaaggtgc tgactttatc
    15961 tataccgatg tatgggtgtc gatgggtgaa gcaaaagaaa aatgggctga acgcattgca
    16021 ttactgcgtg attatcaggt gaacagcaag atgatgcagt tgaccggtaa cccggaggtc
    16081 aaattcctcc actgcctgcc cgcgttccat gatgaccaaa cgacgcttgg caagaaaatg
    16141 gcggaagagt ttggcctaca tggcggaatg gaagtgactg atgaggtctt cgaatctgcc
    16201 gccagcattg tttttgatca ggcggaaaac cgcatgcaca ccatcaaagc ggtgatggtc
    16261 gcgacactca gtaaataatt gatatcgcta taaagatgcc gggaaataca gaaacatccc
    16321 ggcgtcaaat tattttatgc agtgatagtt tttttcttcc agccaaattt ttctattgct
    16381 atacgtaaat tttccggagg atttagtgta tttaaacgct gttggtcaat aaatggcgat
    16441 acattaacct ccgatttcaa gatagtttgc atcattctaa acctaagttt taattcatta
    16501 ttaagttttt cttcaataac atcttttggt tgatttttca taatctcttc attagactct
    16561 ttattattaa aattataccc taacttatca aaataaatct tttcatcctc atagctctta
    16621 agtaac
//
LOCUS       DAZFAB010000100        16288 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZFAB010000100 DAZFAB010000000
VERSION     DAZFAB010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16288
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.100"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(282..707)
                     /gene="arsC"
                     /locus_tag="ACHJWA_003696"
     CDS             complement(282..707)
                     /gene="arsC"
                     /locus_tag="ACHJWA_003696"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFO4310215.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTTLG
                     TRLCRPSEVVLEILPDAQKGAFTKEDGEKVVDEAGKRLK"
     gene            complement(720..2009)
                     /gene="arsB"
                     /locus_tag="ACHJWA_003697"
     CDS             complement(720..2009)
                     /gene="arsB"
                     /locus_tag="ACHJWA_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFO4310216.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(2063..2416)
                     /gene="arsR"
                     /locus_tag="ACHJWA_003698"
     CDS             complement(2063..2416)
                     /gene="arsR"
                     /locus_tag="ACHJWA_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFO4310217.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPSWAAKIIEQAWRCEQEKVQVIVRN
                     LARQNCSVDSKNTCS"
     gene            3160..3243
                     /gene="dinQ"
                     /locus_tag="ACHJWA_003699"
     CDS             3160..3243
                     /gene="dinQ"
                     /locus_tag="ACHJWA_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFO4310218.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3297..4649)
                     /gene="gorA"
                     /locus_tag="ACHJWA_003700"
     CDS             complement(3297..4649)
                     /gene="gorA"
                     /locus_tag="ACHJWA_003700"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFO4310219.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNVEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(4721..5563)
                     /gene="rlmJ"
                     /locus_tag="ACHJWA_003701"
     CDS             complement(4721..5563)
                     /gene="rlmJ"
                     /locus_tag="ACHJWA_003701"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFO4310220.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEPYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKSDGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            5766..7808
                     /gene="prlC"
                     /locus_tag="ACHJWA_003702"
     CDS             5766..7808
                     /gene="prlC"
                     /locus_tag="ACHJWA_003702"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFO4310221.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQVLREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            7816..8568
                     /gene="rsmJ"
                     /locus_tag="ACHJWA_003703"
     CDS             7816..8568
                     /gene="rsmJ"
                     /locus_tag="ACHJWA_003703"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFO4310222.1"
                     /translation="MKICLIDETGTGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(8617..10086)
                     /gene="dtpB"
                     /locus_tag="ACHJWA_003704"
     CDS             complement(8617..10086)
                     /gene="dtpB"
                     /locus_tag="ACHJWA_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFO4310223.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGTFTLFYMSINIGSLIAL
                     SLAPVIADKFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            10252..10305
                     /locus_tag="ACHJWA_003705"
     CDS             10252..10305
                     /locus_tag="ACHJWA_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310224.1"
                     /translation="MVLNIVLAGDFDCERRP"
     gene            10283..10336
                     /locus_tag="ACHJWA_003706"
     CDS             10283..10336
                     /locus_tag="ACHJWA_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310225.1"
                     /translation="MIANADRRPDKAFTLIR"
     gene            complement(10403..10837)
                     /gene="uspA"
                     /locus_tag="ACHJWA_003707"
     CDS             complement(10403..10837)
                     /gene="uspA"
                     /locus_tag="ACHJWA_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFO4310226.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11228..11563
                     /gene="uspB"
                     /locus_tag="ACHJWA_003708"
     CDS             11228..11563
                     /gene="uspB"
                     /locus_tag="ACHJWA_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFO4310227.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(11634..13133)
                     /gene="pitA"
                     /locus_tag="ACHJWA_003709"
     CDS             complement(11634..13133)
                     /gene="pitA"
                     /locus_tag="ACHJWA_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFO4310228.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            13365..14567
                     /gene="yhiN"
                     /locus_tag="ACHJWA_003710"
     CDS             13365..14567
                     /gene="yhiN"
                     /locus_tag="ACHJWA_003710"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFO4310229.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTVRLRSEVLSVAKDDTGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSC"
     gene            complement(14885..15937)
                     /gene="yhiM"
                     /locus_tag="ACHJWA_003711"
     CDS             complement(14885..15937)
                     /gene="yhiM"
                     /locus_tag="ACHJWA_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HFO4310230.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
BASE COUNT         4200 a         4105 c         4218 g         3765 t
ORIGIN      
        1 tgataaatca taatccagta cgtttttaaa ctgctgcact gtatcgctga tcactaagca
       61 gctcactgat acatcatgct ggacgcgata attggaatct cacgcaggga gtaatgtgta
      121 aatagcaaaa tcgcctgatg cgctacgcct gtcaggccta caaataacat acaatttatt
      181 gaattttcat gactttgtag atcggataag acgcgttagc gtcgcatccg acataaataa
      241 agcgcacttt tctaacaacc tgtggggggg atatagccgc tttatttcag gcgcttaccc
      301 gcttcatcaa ccactttctc gccatcttcc ttggtgaatg cgcctttttg cgcatctggc
      361 agaatttcca gcaccacttc tgaagggcgg cacaggcgag ttcccagcgt cgtcaccaca
      421 atcgggcgat taatcagaat cgggtgctga agcataaagt cgattaaccg atcgtcagta
      481 aatttatctt ccgcaaggcc cagctcctca tacggttcga cgtttttacg cagcagcgcg
      541 cgtacggaaa tccccatatc ggcaatgagt ttgaccagtt catcgcgcgt tggcggagtt
      601 tccagataat ggataatggt cggttctgtg ccgctgttgc ggatcatctc cagcgtatta
      661 cgcgacgtgc cgcaggccgg gttgtgataa atggtaatgt tgctcatatc agtatctcat
      721 tacaaagtga aagagagacg tagcgccagc gcagccagcg tcacaaacag cacaggcagg
      781 gtcatgataa tccctgtacg gaagtaatat ccccagctga tagtcatatt cttctgcgaa
      841 agtacgtgca gccagagcag cgttgccagg ctaccaatcg gggtgatttt cggccccaaa
      901 tcgcagccaa tcacgttggc ataaaccatc gcttctttga taacgccaga tgccgtgctg
      961 ccatcaatgg acaacgcgcc aaccagtacc gtcggcatat tgttcataat agaagagagg
     1021 aaggcggtga ggaatccggt gccgagcgtc gcggcccaca ggccgttatc cgccagcacg
     1081 ttgagtacgc cagaaagata ttccgttaat ccggcattgc gcaggccata aaccaccaga
     1141 tacatgccga gcgagaagat gacaatctgc cagggcgcac cgcgcagcac tttacccgta
     1201 ttaatcgcat gaccgcgttt agcgacgaca aataatatca acgcgcccac tgccgcaatg
     1261 gcgctgaccg gaatgccgag cggttccagg acgaaaaatc ccaccagcag aagcagtaaa
     1321 acaacccagc cagttttgaa cgtagcagga tctttaatcg cttctgcggg agatttcagc
     1381 agcgccatgt cgtagttctg cggaatatct ttgcggaaat agagatgcaa catcaccagc
     1441 gtggcaacaa tcgcggcgat atccaccggc accatcaccg aggcatattc gcgaaagccg
     1501 aggccaaaga aatctgcgga aacgatattc accaggttgg agacaataag cggcaggctg
     1561 gcggtatcgg caatgaatcc ggccgccatc acaaacgcca gcgtagtgcc tttactgaac
     1621 cctaaagcca gcagcatggc gatgacaatc ggtgtcaaaa taagcgccgc gccatcattg
     1681 gcaaacaggg cggcaacggc agcaccgagc aggacaatcc aggtaaacag caagcgaccg
     1741 cgtccattgc cccagcgtga aacgtgcagt gccgcccatt caaaaaagcc ggactcatcc
     1801 agcagcaggc tgatgataat aacggcaata aacgcagccg tcgcgttcca gacgatattc
     1861 cacaccaccg gaatatcgcc cggatgtact acgcccgtaa ctaacgccag tactgcgccg
     1921 agcgttgcac tccagccgat gcctaaacct ttcggctgcc agataaccaa tacaatggtc
     1981 aggacaaaga tagcgcctgc cagtaacata atgcctcccg gaaaaacaca tgtgcaaatc
     2041 catatgtgtt tagataaatt ttttaactac aagtgttctt actgtctacg gaacaatttt
     2101 gtctggccag gttgcggaca atcacctgaa ccttttcctg ttcgcatcgc caggcctgct
     2161 caataatttt cgcggcccat gatggaatat gcggtgataa gcggtaatga acccacttac
     2221 cctgcttgcg atccagcaac aaaccgcttt cacgaagaag cgccagatgg cgggagatct
     2281 tgggctgcga ctggtcgaga gcagtgcaga gatcacagac gcataactct cccagctcgc
     2341 tgagcagtaa aacgatgccc agacgggttt catcggcaag aattttgaac agttggatgg
     2401 gtaacagaaa tgacatattg cgctcctgat tgttgctggt agtgtctctc ttcgaagcgg
     2461 ataagtcaaa aacatatatg gtttaacgaa tgtgtatgtg cagaggaagg taataggtgt
     2521 gaattaagcg ttggctattc atttgaaagg aggtctgaat caggacgtaa aaaaaccgac
     2581 tttgcgtcgg ttttttactt tccagcccag agttggtggc tctggctgga gtgagaatgc
     2641 ccacatctaa cggttcaata ctattagcca gtggctaaca ttgcaagcgt tagcgatttc
     2701 ttcagctgga gaagggacag tttgctatac atgttggtga aagaggattt atatactttc
     2761 atcgggtcgc tgccgtacca ggctttgtcc gaatccggat gtaaaaaaac cgacttcgcg
     2821 tcggtttttt tactcttcag ccctgagttg gtggctctgg ctggagtgag aatgccatca
     2881 tctaacgcat caatactaat accatactga aactattgca aggacatgct ggttttataa
     2941 cctgaatata ctgtatgatt atccagttag ctctgaggca ttttcactct ggcaatgcgc
     3001 ataaacgctt tcaaagtcct gttcagtggt acgggtggtg ccgttaactg atgctctggc
     3061 cggagtgaga ataccattat ctaacaatag ggcatgcgcc gtgacaggcg atggatgagt
     3121 aagcggatgc attctcactc catcgtatgg agaacacggg tgattgataa agcaatcatc
     3181 gttcttgggg cgttaattgc gctgctggaa ctgatccgct ttctgcttca gcttctgaac
     3241 tgatagcgga aacgtaatta agggcaaaga gcacaccact ctttgccctt taacatttaa
     3301 cgcatggtca cgaactcttc tgccgccgtt gggtgaatgg cgacggtatt atcgaagtct
     3361 tttttggttg cgcccatctt cagtgccacc gcgaagccct gcaacatttc gtccatacca
     3421 aaaccaatgc cgtgaatacc gacaatcttc tcttctggcc caacgcacac cagcttcatg
     3481 cggcacggct ggcggtgagt ggtgacggcg gtatacatcg cggtgaaaga ggatttatac
     3541 actttcacct gatcgtcgcc gtactgctcg cgcgcctgcg gttccgttaa accaacagta
     3601 ccaatcggcg gatggctgaa gaccacggtc ggaatgttgc tgtaatccag atgctcatcc
     3661 ggcttgttat taaacaggcg ttcagagaga cggcgacccg ctgcaactgc caccggtgtc
     3721 agttctactg cacccgtgtt atcgcccacc gcgtaaatac cttcaacgtt ggtgttttga
     3781 tatttatcga cgacgatata gcctttttcg ttggttttaa cgccagcggc ttcgaggttg
     3841 atgttgtcat tggcaggctc gcggccaatc gcccaaatca ggcaatccac cgtttcactg
     3901 cgaccatctt ccagctccag cgtcaggcta ccatcggcat ttttcactac cgctttcggg
     3961 atggcgttgg tgtgcaactg cgggccttcg gcgttcatca cttcgaccag cgtttcagag
     4021 atcatcgggt cgaagctgcg cagcggcgca tgtttacgca caaacagatg cgttttcgcg
     4081 ccgaggccgt taatcacgcc agccagctca acggcgatgt aacccgcgcc aacaaccgcc
     4141 acacgctctg gcaaagcagg aagggcaaag aaaccatcag aatcaatacc gtattccacg
     4201 cccggaatat ccgggtggct cggacgaccg ccggtggcga tcagaatatg atcggctgtg
     4261 atggtttcac cgttaacttc cagcgttttg gcatcaacga agcgggcaaa gcctttgatt
     4321 acatcaacgt tatttttacc gagcacgttt tcataggaag tatgaatacg gtcgatatag
     4381 gcggtacggc tggcgatcaa cgtttcccag ttgaatttat tgatagtggt atcaaaacca
     4441 taatccgggc cgtacatatg gatcgcttca cggatttgcg ccgcgtgcca catcactttc
     4501 ttcggtacgc agccgacgtt cacgcaggtg ccgcccagct ctttggcttc aatcagcgcg
     4561 catttctgac cgtacatagc cgcgcggttg atggaggcga taccgccgct gccgccgccg
     4621 atggcgatgt aatcatagtg tttagtcatg acagagtgtc cttatcgttg attaccgcga
     4681 ttgtagcgcg agacgtaata ggtgccagca atggctgcaa ttactctggc acgatccagc
     4741 ttacggtggc gtgcccggtg ccagctggaa ccagtttgct gtgcagccac ggcagcacgt
     4801 tattcatctg ctgttccagt ttccacggcg ggttaatcac aatcatgccg gaagcggtca
     4861 tgccacggcg atcgctgtct ggcagtaccg ccagttcaat ttgcagaatt ttgcgaatgc
     4921 cggtcgcttc caggtcgtgg atcatgcgct taatttgctg gcgcagcacc accggatacc
     4981 acaatgcgta agtgccagtg gcgaaacgtt tgtaaccttc tgctatcccg ctgactaccg
     5041 cctgatagtc ggttttcatt tcatacggcg gatcgataag gattaaacca cggcgagaaa
     5101 ccggcggcag tttggcctta agctgctgga aaccgtcgga tttttcgacg cgcgcacggc
     5161 tgtctttctg aaattcagaa cgcagcaacg ggtaatcgct cgggtgcagt tcggtcagtt
     5221 gcaggctgtc ctgttcacgc agtaactgac gggcaatcaa cggtgaaccg gggtagtaac
     5281 gcaactgacc gctacggttg aagtgtttta ccacattgat gtacggctcc agctccgcag
     5341 gcaaatcgtc ctgctgccag atacgggcga taccttcgag atattcgccg gtacgctcgg
     5401 catgttcgct gcctaactga taacgcccgg cccctgcgtg ggtgtcgaga tagagaaacg
     5461 gtttatcttt ctctttcagc gactcgatga tcaggctctg aacggtatgt ttaaggacgt
     5521 cggcgtggtt gccagcgtga aagctgtggc gataactgag catgggtaaa ggtgttccgg
     5581 taagtaaaaa tcggcccgta ttcggggcgc acaaaagcct atcaggacag tataccgaaa
     5641 agaggccgcc gccgcgaaag cgtaacgttt ctcattgaaa ttcactacac ttaaccccat
     5701 gctacaaaca ttatgtaaag cgcctgttga gcgcttcctt aacctcttta accaggactg
     5761 cgctaatgac gaatccgtta ctgactccct ttgaattgcc tccgttttct aaaattctcc
     5821 cggaacatgt cgttccagcc gtgactaagg cattgaatga ctgccgcgaa aatgtggagc
     5881 gcgtagtagc gcaaggagca ccgtacacct gggaaaatct ctgccagccg ttggcggaag
     5941 tggacgatgt gttggggcgt attttctccc cggtcagcca cctgaactcg gtgaaaaata
     6001 gcccggaatt gcgcgaagcg tacgaacaaa ctctgccgct gctgtcggaa tacagcacct
     6061 gggtagggca acatgaaggg ctgtataaag cgtaccgcga cctgcgcgat ggcgatcatt
     6121 acgccacgct gaacacggct cagaaaaaag cggttgataa cgcactgcgc gattttgaac
     6181 tctcaggcat cggtctgccg aaagagaaac aacagcgtta cggcgaaatt gccactcgtc
     6241 tttctgaact gggcaaccag tacagcaaca acgtccttga tgcgacgatg ggctggacta
     6301 aactcgttac cgacgaagcg gaactggcgg ggatgccaga aagcgcgctg gctgcggcaa
     6361 aagcccaggc cgaagcgaaa gagctggaag gctacctgtt gacgctggat atcccaagct
     6421 acttgccggt aatgacctac tgcgacaacc aggtcttgcg tgaagagatg tatcgtgctt
     6481 acagcacccg cgcttcagat caaggtccaa acgccggtaa atgggataac agtaaggtga
     6541 tggaagagat cctcgcgctg cgtcacgaac tggcgcaact gctgggcttt gaaaactacg
     6601 cgtttaaatc actggctacc aaaatggcgg aaaacccgca gcaggtgctt gatttcttaa
     6661 ccgatctggc aaaacgcgcg cgtccgcagg gcgaaaaaga gctggcgcaa ctgcgtgcct
     6721 tcgccaaagc cgaatttggc gtggatgagt tgcaaccgtg ggatatcgca tactacagcg
     6781 aaaaacaaaa acagcacctc tacagcatca gcgacgaaca actgcgtccg tacttcccgg
     6841 aaaacaaagc ggttaacggc ttgtttgaag tggtaaaacg tatttacggc atcaccgcta
     6901 aagagcgtaa agatgttgat gtctggcacc cggatgtacg tttcttcgaa ctgtatgacg
     6961 agaacaacga actgcgcggc agtttctacc tcgacctgta tgcccgtgaa aacaagcgcg
     7021 gcggggcgtg gatggatgac tgcgtaggcc agatgcgtaa agccgatggt tcgctgcaaa
     7081 aaccggtcgc gtatctgacc tgtaacttca accgcccggt gaatggtaaa ccggcgctgt
     7141 tcactcacga cgaagtgatc actctgttcc acgagttcgg tcacggcctg caccatatgc
     7201 tgacccgcat cgaaaccgcc ggtgtttccg gtatcagcgg tgtgccgtgg gatgcggtcg
     7261 aactgccgag tcagtttatg gaaaactggt gctgggagcc ggaggcgctg gcgtttatct
     7321 ctggtcacta cgaaaccggc gaaccgctgc cgaaagagtt gctggataaa atgctggcgg
     7381 cgaagaacta ccaggcggcg ctgtttattc tgcgccagct ggagttcggc ctgttcgatt
     7441 tccgcctcca tgccgagttc cgcccggatc agggagcgaa aatcctcgaa actctggcag
     7501 aaatcaagaa actggttgcc gtagtaccgt ctccatcctg gggccgtttc ccgcacgctt
     7561 tcagccatat tttcgccggt ggttatgccg caggttacta cagctacctg tgggctgacg
     7621 tactggcggc agatgctttc tcgcgctttg aggaagaggg cattttcaac cgtgaaaccg
     7681 ggcagtcgtt cctcgacaac attctgagcc gtggcggttc agaagagccg atggatctgt
     7741 tcaaacgctt ccgtggtcgt gaaccgcagc tggatgcgat gctggagcat tacggcatta
     7801 agggctgatc attcagtgaa aatctgctta attgatgaaa caggcaccgg agacggtgcc
     7861 ttatctgttc tggcggcccg ctgggggctg gagcacgatg aagacaacct gatggcgctg
     7921 gtgttaacgc cggaacatct ggaattgcgc aagcgtgatg agccaaaact tggcggcatc
     7981 tttgttgatt ttgttggcgg agcgatggcg caccgacgca aattcggcgg tggtcgcggt
     8041 gaggcggtgg cgaaagcggt gggcattaaa ggcgattatt tgccggatgt ggtggacgct
     8101 accgcagggc tggggcgcga tgcctttgtg ctggcttcgg tcggctgccg cgtgcggatg
     8161 ctggagcgta atccagtcgt tgccgcgcta ctcgacgacg gcctggcgcg tggatatgcg
     8221 gatgcggaaa tcggcggctg gttgcaggag cgtttgcagt taattcacgc ctccagcctg
     8281 acggcgctga ccgatattac cccgcgtccg caggtggtct atctcgaccc gatgttcccg
     8341 cataagcaga aaagcgcgct ggtgaaaaaa gagatgcgcg tctttcagtc gctggtggga
     8401 ccggatcttg atgccgatgg attactggag cctgcacgct tgctggcgac caaacgcgtg
     8461 gtggtaaagc gcccggacta cgcgccaccg ctagcgaatg tcgccacgcc aaacgcggtt
     8521 gtgactaaag ggcatcgctt tgatatctat gcaggtacgc cagtgtaaaa gtaaagcagg
     8581 gtagcgataa cgctaccctg ctttctgcaa gaataattaa tggctttccg gcgtcgcaat
     8641 catgcgtttc agccacggca ccatcaacag catcactact gcaacgccca gcgtgaccag
     8701 accaatctta ccaaacacgt tggtatagac aggcaacgtc tcaagcggat cggtaatgtt
     8761 gtctggtact gcggtaaatg ttgccacata gccgcccagc aagaacgcgg cagcctgtgt
     8821 caggaaccac atcccgagaa taaagcccat caaatgctgc ggcaccagag cagcaatcat
     8881 cgccaggcca agggcgctaa taaacagttc acctaagctc tggaataagt acaccagcac
     8941 gataaaccat ggcgatgtca gcccttgtgc atccgcaaac cacattcccg cagctgccgc
     9001 cgtcaaaaag cccagtgagc acataaacat gccgagagta aatttcatcg gcatcgagag
     9061 gtctttgcct ttgttaccca gatgcgtgta aatgcctgcc agtattgggc tggcgagtac
     9121 cacccagaac gggttaagcg cctggaagct gaccgggttg atggaaaaac cgagaatttc
     9181 atgatgcacg ttgttgatgg caaagaagtt cagcgatgtt ggcatctggg cgtagagaat
     9241 gtaaaacacc accgcttcga gcatcaggac aaaggcgaca aacattttat tgcgcccggt
     9301 tttatccagc ttgaatgcct gacgaaagaa gatgatggtg acgacgatgg agagaacaat
     9361 cagcaccaga ttggcgactt ctacgttgtg catcagccag gcgcagacga agatcatcac
     9421 cacgctgcca agtaatacgt acaacagttt gctgaagctc attggccgga agtcgggctc
     9481 agaaccaatg tctttcacca ttccacgaca ggcgatgtaa accagtaatg cgataattaa
     9541 ccccgcaccg cacaggttgt aggtgactga atagccgaat ttatcagcga tcacaggggc
     9601 cagcgataac gctatcaacg agccgatatt gatcgacata tagaacaggg tgaatgtgcc
     9661 atcaaggcgc ggatctttcg gcggatagca cttcgaaagc aagctggctg ggttagcttt
     9721 aaacaggccg ttaccgacag cgatagtccc cagggcgatg aaaatcaggt caggcttaag
     9781 tagcgacatg ccggtcatga agtagccaat cgccagcaca agtgctccaa gaacgatggt
     9841 gcgtttggtc cccagcaggt ggtcgccgac atagccgcca atggaaatga ggccatagac
     9901 cagtgcagca aaagcaccaa aagtgacaaa agcctgctct tgcgagaatc caagctgttt
     9961 aacgaagaaa accgccagta cgccctgcac gccgtagtag ccgaatcgct cccataactc
    10021 gacaaaaaag atcatgaaaa atgggcgagg ttgctgcagc atccccatgg gtgttgttgt
    10081 attcatattt attaaccttc caataccatc ctgaaaagta tgaacgcgta agggataacg
    10141 cgcgaaaaag tttacatctg tcagagattc ttaatattga tgcggtgaat agattaatac
    10201 actattacaa cagaaaataa ccagctgatc acactaatga tggaaaatat gatggtgtta
    10261 aatattgtat tggctgggga ttttgattgc gaacgcagac cgtaggccag ataaggcgtt
    10321 tacgctgatc aggtaacaag gcgcgacaaa aagcaaaacg cccgcatatg cgggcgttca
    10381 ggaacacgtc gtggagggaa gattattctt cttcgtcgcg cagcggaaca atcagcatat
    10441 caacgtgaac ggtgttgatc agctgacgtg cggaagacat cagtttgctc cagaagtcct
    10501 ggtggtgacc acaaaccact agatccatat cgtatttctt gattgcatcg accagaacct
    10561 ggcccaggtc gccgctgccg ctcagggttt cagtgattgg gtagcctgca ttagtggaaa
    10621 gctcggtcag cgcatgatgt gtctcttcag agatgcgttt ctgcatatca ccaagattca
    10681 catcaataag cccggtatat aggtcagagt agtttacatc tacgtggatc agagaaactt
    10741 tcgcattgta tgggcgagcc atagagactg ctttctctac cagaactttg ctttccgggg
    10801 agagatcgac cgcgatgaga atgtgtttat aagccatagt gttactcctt ccataaagtt
    10861 gtcgatgact ggccagctag cgtttcttgt gccgttacgt cacccgcgtc ctgcgaacta
    10921 cgtgcgcggg gctaacccag ccgtaaagat tcagtgttaa gaccatcctt accttatagc
    10981 gacccggatg atccgtcaat ccgccttgct tacccgttag ataaacaaat tttcgacatg
    11041 aatatattga tagtggttaa ccttctggaa aaaaaacaac ctgatctcct acactatcta
    11101 tagagccgct cggatgttgg tcaccacaaa agcggtttct ccaggacctg gtgttaactg
    11161 attggcagcg tatcgggagc ggtagccccg gggtggaact ccgcgggcag gtcgccgggg
    11221 aggaggtatg ataagcaccg tcgcattatt ttgggcttta tgtgtcgttt gcattgttaa
    11281 catggcgcgc tatttctcat cactacgcgc gttgttagtg gtactgcgta actgcgatcc
    11341 attgctctat caatatgttg atggaggggg cttttttacc tcacatggcc aacccaacaa
    11401 acaggtgcgt ctcgtttggt atatctatgc ccaacgttat cgcgatcatc acgatgatga
    11461 gtttattcgc cgctgtgagc gggtgcgtcg gcagtttatt ctgaccagcg cattgtgtgg
    11521 tctggtggtg gtcagcctga ttgccttgat gatttggcat taagcacttc agcaataata
    11581 acgcataaaa aaagcgggcc agtaaactga cccgctcgtt ttggtgcgta ctattacagg
    11641 aactgcaagg agagccagta aagcccgccg gaaagcagta ccgcagccgg aagggtaaac
    11701 acccaggcca tcagaatgct ggtcacggtt ttacgctgta agccgccacc atctaccacc
    11761 atcgtccccg cgacagaaga ggagagtacg tgagtagtgg aaaccggcat cccggtataa
    11821 ctcgccaggc cgatagacac tgccgcagtc atctgggcag acatcccctg agcgtaggtc
    11881 atgcctttct taccgatttt ctcaccgata gtcgttgcca cacgacgcca gccaatcatc
    11941 gtaccgatac ctaacgccag cgcgaccgcc atgatgatcc acaccggtgc atactcgatg
    12001 gtgctaagca tgtcggactt cagtttcttc aacaggcgct gatcgtcagc actcacgcca
    12061 ggcatcttca ccactttgtc gatagtgtca gaaacgcaca gcataatgcg gcgcatctgg
    12121 ctacgttgat caagcgacag cttgtcgtag ctttccacat cggtggtcaa catacctttc
    12181 aggcggttga gcgcgttaat ggtattcgac ggatggcagt ggaactccgc aggttgcgtt
    12241 gcgccagctt ccggagccgg tactaactga tcagcaccag tagcttgttt cagcagcgca
    12301 ggatgctgct caaagtaagc ttcgacgttg ttgatggcat cacgggtacg ggtgatttcg
    12361 tagccagtgg cattcatgtt caccacgaag cctgctggtg cgacaccaat caataccaac
    12421 ataaccagac caatgccttt ctgaccatcg tttgcgccgt gcgaaaacgc cacgccgata
    12481 gcggaaagga tcagcgcgat acgcgtccag aacggcggct ttttcttgcc gtctttcttt
    12541 tcacgctccg ctggggtcag gtggatacgg gcgcgtttct tggtgccgct ccagtagcga
    12601 cgcagcaaga aaatcagacc gccagcaaac accaggccga caatagggga aacgatcaga
    12661 gaaccgaaaa tacttaatac tttcgggata ttgagtgcat ccaccactga cgtcccggtc
    12721 atcaacgcat tggttaaacc aatcccgatg atcgcgccaa tcagcgtatg agagctggat
    12781 gcaggcaaac caaagtacca ggtacccagg ttccagataa tcgccgccag caacatagag
    12841 aacaccatgg caaggccatg agacgatccc atattaagca gcagatccgt cggcagcata
    12901 tgcacaatgg cataggcaac actcagacca cccagcaaaa cacccaaaaa gttgaacacc
    12961 gctgccataa ccacggcgag ctgagaacgc atcgcgcggg tatagataac ggttgccacg
    13021 gcgttggctg tgtcatggaa accattgatg gcttcgtaga acagcacaaa agccagtgca
    13081 agcaataata acagcccggt atgcaaatcc aggccagcaa acaaatgtag cataggacgt
    13141 tacgccattt tgaggacatg aacgcggcgc attatcagtg actttcgcgg cgcgggcaaa
    13201 gtgaaatata gacttttttt gatttgcctc ctgtatggat ttcactcaaa aaataattat
    13261 cttatataat tcaggcaaat acttcctttt agtaatattg atgctggtgc gaccactgag
    13321 gaatctttac aattcacgcc cgttttttct aagaggagcg caacgtggaa aggtttgatg
    13381 ccattattat aggcgctggt gcggcgggta tgttctgttc tgcgctggca ggtcaggcag
    13441 gacgccgggt tctgctgatc gataatggta aaaaaccagg gcgcaaaatc cttatgtctg
    13501 gcggtgggcg ctgcaacttt accaaccttt atgtcgaacc gggcgcttat ctgagccaga
    13561 atccgcattt ttgtaagtct gcactcgcgc gttttaccca gtgggatttc attgatctgg
    13621 tcaataaaca cggcatcgcc tggcacgaga aaacgttagg gcaactcttc tgcgatgact
    13681 ccgcgcagca gattgtcgac atgctggtgg atgagtgcga gaagggcaat gtgaccgtca
    13741 gattgcgtag cgaagtgctg agtgtggcga aggatgatac aggcttcacg cttgaactga
    13801 acggcatgac tgtcggttgt gaaaagctgg tcatcgcgac cggcgggctg tcaatgccgg
    13861 gcctgggcgc gtcgccgttt ggttataaga ttgccgaaca atttggcctc aacgtgctgc
    13921 cgacccgcgc gggtctggtg ccattcactc tgcataaacc gttgctcgaa gagttacagg
    13981 tgctggcggg cgtggcggtg ccttccgtga ttaccgctga aaacggcacc gttttccgtg
    14041 agaacttact cttcacccat cgcggcttgt ctggaccggc ggtgttgcaa atttccagct
    14101 actggcaacc gggggaattt gtcagtatca atctgctacc ggatgtggac ctcgaaacct
    14161 tcctgaatga gcagcgtaac gcacatccga atcagagcct gaaaaacaca ctggcggttc
    14221 atctaccgaa gcggttggtt gaacgcttac agcaactcgg gcaaatcccg gatgtttcgc
    14281 taaaacagct caacgtgcgt gaccaacagg cactgattag cacattgacc gactggcgcg
    14341 tacaacccaa cggcactgaa ggctatcgca ctgccgaagt gacgctcggc ggcgtggaca
    14401 ccaacgaact ctcttcacgg acgatggaag cgcgcaaagt gcctgggctg tactttatcg
    14461 gcgaagtgat ggacgttacc ggctggctgg ggggctataa cttccagtgg gcgtggtcga
    14521 gtgcgtgggc ttgtgcgcag gatttgattg cagcgaagtc gtgctgagct atttttccta
    14581 aagatgaaaa ttattagagt cgcattaaaa atgagctaat tttgatagtg gctatcttgt
    14641 gattattttc taatgagccc gtgaactgaa ccccccatgc ttaatataag gtggagggaa
    14701 aggtgattga aaactcaccc agtgggctga tctttataaa atatgaacag gttgaaaaaa
    14761 aaacagtaac tttgctgttt tttatactta accactatgc attaatgctg cgttatttca
    14821 ttctacctaa gaaaaaccag cgttacgcaa atggttaacg ctggttttat ccggtacgtt
    14881 gcaattattt tttagcagaa cccgcttcta atattgaaac gattgagaac aacgtaaagc
    14941 ataccgctcc caaaccgacc agaacccgcg aaggaataaa atatcccata tcggtctgcg
    15001 ccatttccgc aagaaacgat gctaggaaaa ggcaaaacag gcaggtgaag acgggaatca
    15061 tcggtattct gttggctaac gaacaggtac gtctccagac cagtgccagt agccagactt
    15121 ttgagaatat gctgtaacag atcattccca agcaaatgag gataataccg ggagccagtc
    15181 gggcgcttgc atcagaactg actaagacgt atatgccctg cagcaccgtg attgagccga
    15241 gaaaaataac ccaataacac cacagccagt gctctttcgt tgaaaacgta ttacgtgttt
    15301 gatggacgat tgtggcaaca aggccaatta gacaggcaca gattgcggtt aacccgagca
    15361 atacgtgacc ggcgacataa tgcggagtta tgtcggcact acgtaacagc gtaatagacc
    15421 aaatgaaacc gagcagggtg agtaacacgg gaacggcaat gaggcaatta ccgattaatg
    15481 aagaataagc ctgtaccggt gttccgtcgc tcttgctccc cgctgcgttt ttaggaatga
    15541 gcagaaagtg accggatgac gctgccaccg tcgatacaca ggcggcaatc ataccaacgc
    15601 cgaaaataac atggccggcg acaaactcgt ctgccatcac atcattacct gctaacagtg
    15661 cccaacccca tatcatggtg ataattgacc ccgcatagcc aataatgggg aacaatgtat
    15721 tgtaaaacgt attaacgccg cgcgtgagct gcgaaatgat aataaatgcg gtagtgaata
    15781 atgccaggca tatggctgcc agagaaatga gcacatgacc cgcgacgaaa tactcgcttt
    15841 gccctgaact ttccagcaca aagccaccta acgcgataca aatcaagccc ataattaaag
    15901 ggattaattt gaaaagccac ccgatatata tgttcacttt aaactcctca ggtatccctg
    15961 ctaatattga aatgtgcatt aatgataatt tcagcctgtt aatacaatga cgagatatca
    16021 ttaaagacaa atgcatagca taactatagc ggggtttact ttgcagagcg ctacttttca
    16081 gcgttcaaaa ggccatgata aataaatatt tatcctaaga ttaattctta ttctgttgtt
    16141 ttttacaatt atcttccagg gagggattct ttttcattat ttggtaaaag accggatggt
    16201 ctcggtgctt aaaatcagcg agtatatacg catcattgct ttatatcgtc aaaaaatatg
    16261 tctttcagac atgtatcatt tattttgt
//
LOCUS       DAZFAB010000101        15537 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZFAB010000101 DAZFAB010000000
VERSION     DAZFAB010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15537
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.101"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            125..328
                     /locus_tag="ACHJWA_003712"
     CDS             125..328
                     /locus_tag="ACHJWA_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFO4310231.1"
                     /translation="MSSIFLTENELQTLTGCKYASHQRNWLIKNGLPFYTNRSGKPIV
                     SRELFTCIKTLPPREAEPDFGAI"
     gene            328..1375
                     /locus_tag="ACHJWA_003713"
                     /pseudo
     CDS             328..1375
                     /locus_tag="ACHJWA_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1403..1492)
                     /locus_tag="ACHJWA_003714"
     tRNA            complement(1403..1492)
                     /locus_tag="ACHJWA_003714"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1456..1458),aa:Ser,seq:cga)
     gene            1586..2383
                     /gene="mtfA"
                     /locus_tag="ACHJWA_003715"
     CDS             1586..2383
                     /gene="mtfA"
                     /locus_tag="ACHJWA_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFO4310232.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            2484..2559
                     /locus_tag="ACHJWA_003716"
     tRNA            2484..2559
                     /locus_tag="ACHJWA_003716"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2517..2519,aa:Asn,seq:gtt)
     gene            2873..10855
                     /locus_tag="ACHJWA_003717"
     CDS             2873..10855
                     /locus_tag="ACHJWA_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin-like protein
                     YeeJ"
                     /protein_id="HFO4310233.1"
                     /translation="MATKKRSGEEINDRQILCGMGIKLRRLTAGICLITQLVFPMAAA
                     AQGVVNAATQQPVPAQIAIANANTVPYTLGALESAQSVAERFGISVAELRKLNQFRTF
                     ARGFDNVRQGDELDVPAQVSENNLTPPPGNSSGNLEQQIASTSQQIGSLLAEDMNSEQ
                     AANMARGWASSQASGAMTDWLSRFGTARITLGVDEDFSLKNSQFDFLHPWYETPDNLF
                     FSQHTLHRTDERTQINNGLGWRHFTPTWMSGINFFFDHDLSRYHSRAGIGAEYWRDYL
                     KLSSNGYLRLTNWRSAPELDNDYEARPANGWDVRAEGWLPAWPHLGGKLVYEQYYGDE
                     VALFDKDDRQSNPHAITAGLNYTPFPLMTFSAEQRQGKQGENDTRFAVDFTWQPGSAM
                     QKQLDPNEVDARRSLAGSRFDLVDRNNNIVLEYRKKELVRLTLTDPVTGKSGEVKSLV
                     SSLQTKYALKGYNVEATALEAAGGKVVTTGKDILVTLPAYRFTSTPETDNTWPIEVTA
                     EDVKGNFSNREQSMVVVQAPTLSQKDSSVSLSSQTLSADSHSTATLTFIAHDAAGNPV
                     IGLVLSTRHEGVQDITLSDWKDNGDGSYTQILTTGAMSGTLTLMPQLNGVDAAKAPAV
                     VNIISVSSSRTHSSIKIDKDRYLSGNPIEVTVELRDENDKPVKEQKQQLNTAVSIDNV
                     KPGVTTDWKETADGVYKATYTAYTKGSGLTAKLLMQNWNEDLHTAGFIIDANPQSAKI
                     ATLSASNNGVLANENAANTVSVNVADEGSNPINDHTVTFAVLSGSATSFNNQNTAKTD
                     VNGLATFDLKSSKQEDNTVEVTLENGVKQTLIVSFVGDSSTAQVDLQKSKNEVVADGN
                     DSATMTATVRDAKGNLLNDVKVTFNVNSAAAKLSQTEVNSHDGIATATLTSLKNGDYT
                     VTASVSSGSQANQQVIFIGDQSTAALTLSVPSGDITVTNTAPLHMTATLQDKNGNPLK
                     DKEITFSVPNDVASRFSISNSGKGMTDSNGTAIASLTGTLAGTHMITARLANSNVSDT
                     QPMTFVADKDRAVVVLQTSKAEIIGNGVDETTLTATVKDPFDNVVKNLSVVFRTSPAD
                     TQLSLNARNTNENGIAEVTLKGTVLGVHTAEAILLNGNRDTKIVNIAPDASNAQVTLN
                     IPAQQVVTNNSDSVQLTATVKDPSNHPVAGITVNFTMPQDVAANFTLENNGIAITQAN
                     GEAHVTLKGKKAGTHTVTATLGNNNASDAQPVTFVADKDSAVVVLQTSKAEIIGNGVD
                     ETTLTATVKDPFDNAVKDLQVTFSTNPADTQLSQSKSNTNDSGVAEVTFKGTVLGVHT
                     AEATLPNGNNDTKIVNIAPDASNAQVTLNIPAQQVVTNNSDSVQLTATVKDPSNHPVA
                     GITVNFTMPQDVAANFTLENNGIAITQANGEAHVTLKGKKAGTHTVTATLSNNNTSDS
                     QPVTFVADKTSALVVLQISKNEITGNGVDSATLTATVKDQFDNEVNNLPVTFSTASSG
                     LTLTPGESNTNESGIAQATLAGVAFGEQTVTASLANNGASDNKTVHFIGDTAAAKIIE
                     LTPVPDSIIAGTPQNSSGSVITATVVDNNGFPVKGVTVNFTSNAATAEMTNGGQAVTN
                     EQGKATVTYTNTRSSIESGARPDTVEASLENGSSTLSTSINVNADASTAHLTLLQALF
                     DTVSAGDTTNLYIEVKDNYGNGVPQQEVTLSVSPSEGVTPSNNAIYTTNHDGNFYASF
                     TATKAGVYQVTATLENGDSMQQTVTYVPNVANAEISLAASKDPVIANNNDLTTLTATV
                     ADTEGNAIANSEVTFTLPEDVRANFTLGDGGKVVTDTEGKAKVTLKGTKAGAHTVTAS
                     MAGGKSEQLVVNFIADTLTAQVNLNVTEDNFIANNVGMTRLQATVTDGNGNPLANEAV
                     TFTLPADVSASFTLGQGGSAITDINGKAEVTLSGTKSGTYPVTVSVNNYGVSDTKQVT
                     LIADAGTAKLASLTSVYSFVVSTTEGATMTASVTDANGNPVEGIKVNFRGTSVTLSST
                     SVETDDRGFAEILVTSTEVGLKTVSASLADKPTEVISRLLNAKADINSATITSLEIPE
                     GQVMVAQDVAVKAHVNDQFGNPILNESVTFSAEPPEHMTISQNIVSTDTHGIAEVTMT
                     PERNGSYMVKASLANGSSYEKDLVVIDQKLTLSASSPLIGVNSPTGATLTATLTSANG
                     TPVEGQVINFSVTPEGATLSGGKVRTNSSGQAPVVLTSNKVGTYTVTASFHNGVTIQT
                     QTIVKVTGNSSTAHVASFIADPSTIAATNSDLSTLKATVEDGSGNLIEGLTVYFALKS
                     GSATLTSLTAVTDQNGIATTSVRGAITGSVTVSAVTTAGGMQTVDITLVAGPADASQS
                     VLKNNRSSLKGDFTDSAELHLVLHDISGNPIKVSEGLEFVQSGTNAPYVQVSAIDYSK
                     NFSGEYKATVTGGGEGIATLIPVLNGVHQAGLSTTIQFTRAEDKIMSGTVLVNGANLP
                     TTTFPSQGFTGAYYQLNNDNFAPGKTAADYEFSSSASWVDVDATGKVTFKNVGSKWER
                     ITATPKTGGPSYIYEIRVKSWWVNAGDAFMIYSLAENFCSSNGYTLPLGDHLNHSRSR
                     GIGSLYSEWGDMGHYTTEAGFHSNMYWSSSPANSNEQYVVSLATGDQSVFEKLGFAYA
                     TCYKNL"
     gene            complement(11117..12169)
                     /locus_tag="ACHJWA_003718"
     CDS             complement(11117..12169)
                     /locus_tag="ACHJWA_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFO4310234.1"
                     /translation="MFLVSLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEAMRKKNLKTMLFISQLKAKTNFQVVGLTMKCYSPVCKNASRMDQ
                     KLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIALNLEGSSQ
                     DRTFSLSIAENLIEKIQSETDIPIVIVHGPKGEDKARVLVDCYNNVYRLSLSPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            12483..13799
                     /gene="shiA"
                     /locus_tag="ACHJWA_003719"
     CDS             12483..13799
                     /gene="shiA"
                     /locus_tag="ACHJWA_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFO4310235.1"
                     /translation="MDSTLISTRPDERTVSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQYNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDNQRA"
     gene            13901..15355
                     /gene="amn"
                     /locus_tag="ACHJWA_003720"
     CDS             13901..15355
                     /gene="amn"
                     /locus_tag="ACHJWA_003720"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFO4310236.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT         4258 a         3695 c         3721 g         3863 t
ORIGIN      
        1 cgttaccagg caaaatacgt ttcagccatc tggcaaaaac tgctcccgag tcaggctgat
       61 ttttaatgtt caacccggat cgccgctaac ggcatatagt tagcggcggt tgtgagatat
      121 agctatgagc agcatatttt taaccgaaaa tgaattgcaa acgttaacgg gctgcaaata
      181 tgcaagccac cagcgcaact ggttaataaa aaatgggctt ccgttctata ccaatcgtag
      241 tggaaaaccg atcgtcagtc gggaactgtt tacctgtata aaaactttac caccacgtga
      301 ggctgaacct gatttcggtg cgatctaatg ggaagacgaa ggaaaaatcc cgaacacgaa
      361 aaattaccgc caaaggtata ccccaataaa tatagtgtat ggaaaccgac atccagagaa
      421 tctgtaacct taactgcaat cgaggatggt ttagccgcat tatggaaaaa gtatgaagaa
      481 acggttaacc atcgcgatcg cgcaatgaca tttgggcgtt tgtgggaaaa atttctcgcc
      541 agcgcctatt acagcgagct tagtcctaga actcaaaaag attatctgca acatcaaaaa
      601 aagctgctgg ccgtattcgg taaggtgctg gccgattctg taaaaccaga gcacatcaga
      661 cgatacatgg acaaaagagg cgagcagagt aaaacgcagg caaaccatga aaaaagcagt
      721 atgtcgcgcg tttatagttg ggggtatgag cgaggatacg tgaaggctaa cccatgtgca
      781 ggtgtaagta aattcaaggc caaaaaccgc gaacgatatg taaccgacaa agaataccag
      841 gcagtattaa gcgttgcacc tcttcctgtt tttatcgcaa tggaaattgc ctatctgtgt
      901 gcagcgaggg tttccgatgt gttatcgctg aaatgggagc agattggaaa cgacgggatc
      961 tttatccagc aagggaaaac agggaaaaaa cagataaaag catggagtcc acgattacag
     1021 gcggcgatcg aaaaagcaaa acagttacca acatccgcct atgtaatcag caatcaatac
     1081 ggcaaccgat atatgtacaa aggctttaac gaaatgtggg tagaagcaag aaatcacgca
     1141 ggcaaaattt caggtatttt aaccgacttc acctttcatg atctgaaggc gaaaggaatt
     1201 tcagactatg aaggaagcag ccgggataag caacttttct ctggtcacaa aaccgaaggg
     1261 caagtgctaa tctatgacag gaaggttaaa gtttcaccaa cacttgatgt cccgttacct
     1321 gaaaatattc caagaaaata ttccaagtaa ttccaagtgt gatttttgtc actgacttaa
     1381 tgatgtgtaa gtgattgaat tttggcggag agagggggat ttgaaccccc ggtggagttg
     1441 cccccactcc ggttttcgag accggtccgt tcagccgctc cggcatctct ccgttcagat
     1501 ggttgccatg atgccaggaa atttggcatt ttaacagtcc ctgtccgtgc aattttgttc
     1561 aagtgacgag tttgcgagca aaacgatgat taagtggccc tggaaagtac aagaatcagc
     1621 acatcaaact gcccttccct ggcaggaagc actatcgatc ccccttttaa cgtgtctgac
     1681 ggaacaggaa caaagcaaat tagtcactct tgccgaacgt tttttacagc aaaagcggct
     1741 tgttccttta cagggctttg aactggattc attaagaagc tgccggatag cacttctatt
     1801 ttgcctaccc gttctggagt taggactgga atggctggat ggttttcatg aagtcttaat
     1861 ttatcctgcg ccatttgtgg tcgatgatga atgggaagac gatatcggtc tggtgcataa
     1921 ccaacgtatt gttcagtcag gtcagagctg gcagcaaggg cctatcgttt tgaactggtt
     1981 ggatatacaa gattcttttg atgcttctgg ttttaacctg attattcatg aagtcgctca
     2041 taagctggac acccgtaacg gcgatcgcgc cagcggagtt ccctttattc cgttgcgtga
     2101 ggttgctggc tgggaacacg atcttcatgc tgcaatgaac aacattcagg aagaaatcga
     2161 attggttggc gagaatgcgg cgagcattga tgcttatgct gccagtgatc ctgctgaatg
     2221 ttttgccgta ctttctgaat atttctttag cgccccagaa ctttttgctc ctcgtttccc
     2281 ttcattgtgg caacgtttct gccaatttta tcaacaagat cctttgcaga gactgcatca
     2341 cgctaatgat acagactcgt tttcggcgac gaatgttcat taattaacaa ctttgcagat
     2401 taattaacca attgaaatga cttatgaaat ttagtgttga cagacaaggt accgctaagt
     2461 aatatgcgcc ccgttcacac gattcctctg tagttcagtc ggtagaacgg cggactgtta
     2521 atccgtatgt cactggttcg agtccagtca gaggagccaa attctaaaaa ttcgcttttt
     2581 tagcgcaatg tcactgacct tagttgaaca ttgtttttta acggatagcg ggtttttaac
     2641 atcttaagcg ccctcgacct ttatggttga gggcgttttg ctatgaacgc catcaccatt
     2701 ttcccctcga ttataaaact tgagttattc agtagtctcc cctcttgcaa ctcacatcca
     2761 aaactgccta acgaaaagtt attaattttc aatcatattg ctatcaggat ttacattttt
     2821 tcgctgtgct agaaagggcg catttatgtt agctcgttca gggaaggtaa gcatggctac
     2881 gaagaagaga agtggagaag aaataaatga ccgacaaatc ttatgcggga tgggaattaa
     2941 actacgccgc ttaactgcgg gtatctgcct gataactcaa cttgtgttcc ctatggctgc
     3001 ggcagcacaa ggtgtggtaa acgccgcaac ccaacaacca gttcctgcac aaattgccat
     3061 tgcaaatgcc aatacggtgc cctacaccct tggagcgctg gaatcggccc aaagcgttgc
     3121 cgaacgtttc ggtatttcgg tggctgagtt acgcaaactc aaccagtttc gtacgtttgc
     3181 tcgaggtttt gataatgtcc gccagggtga tgaactggat gtcccggcac aagttagtga
     3241 aaataattta accccgccac cgggtaatag cagtggcaac cttgagcaac agatagccag
     3301 tacttcacag caaatcgggt ctctgctcgc cgaggatatg aacagcgagc aagcggcaaa
     3361 tatggcgcgt ggatgggcct cttctcaggc ttcaggcgca atgacagact ggttaagccg
     3421 cttcggtacc gcaagaatca cgctgggcgt ggatgaagat tttagcctga agaactccca
     3481 gttcgatttt ctccatccgt ggtatgaaac gcctgataat ctctttttca gtcagcatac
     3541 tctccatcgt actgacgagc gtacgcagat taacaacggc ttgggttggc gtcatttcac
     3601 tcccacatgg atgtcgggca tcaacttctt tttcgaccac gatcttagcc gttaccactc
     3661 ccgcgccggc attggcgcgg agtactggcg cgactatcta aaattaagca gtaacggcta
     3721 tttgcgactg accaactggc gcagcgcacc tgaactggac aacgattatg aagcacgccc
     3781 ggccaatggc tgggatgtac gcgcagaagg ctggctaccc gcctggccgc accttggcgg
     3841 taaactggtc tatgaacagt attatggcga tgaagtggcc ctgttcgata aagatgatcg
     3901 gcaaagtaat cctcatgcca taaccgctgg acttaactat acccccttcc cgctgatgac
     3961 cttcagcgcg gagcaacgcc agggtaaaca gggcgaaaat gacacccgtt ttgccgtcga
     4021 ttttacctgg caacctggaa gcgcgatgca gaaacagctt gacccgaatg aagtcgatgc
     4081 acggcgtagc cttgcaggca gccgttttga tctggtggat cgcaacaaca acatcgttct
     4141 ggaatatcgc aaaaaagaac tggttcgcct gaccctgaca gaccccgtga cagggaagtc
     4201 aggagaagtg aaatcactgg tttcgtcgct acaaaccaaa tatgccctga aaggctataa
     4261 cgtcgaagcc accgctctgg aagctgccgg tggtaaagtg gttacaacgg gtaaagatat
     4321 tctggttacc ctgccggcgt accggttcac cagtacgcca gaaaccgata acacctggcc
     4381 gattgaagtc accgctgaag atgtcaaagg caatttttcg aatcgtgaac agagcatggt
     4441 agtcgttcag gctcctacgc taagccagaa agattcctcg gtatcgttaa gtagccagac
     4501 gttgagcgcg gattcccatt caaccgccac actgactttt attgcgcatg atgcagcagg
     4561 taatcctgtt atcgggctgg tgctctcgac gcgtcacgaa ggtgttcagg acatcaccct
     4621 ttctgactgg aaagataatg gtgacggaag ctatacccag atcctgacca caggagcgat
     4681 gtctggcacg ctgacgctga tgccacagct gaacggtgtg gatgcggcta aagcccccgc
     4741 cgtggtgaat atcatttctg tttcgtcatc ccggactcac tcgtcaatta agattgataa
     4801 ggaccgttat ctctccggga atcctatcga ggtgacggta gaactgagag atgaaaatga
     4861 caaacctgtt aaggagcaaa aacagcaact gaataccgca gtcagcatcg acaacgtgaa
     4921 acctggtgtc actacagact ggaaagaaac cgcagatggc gtctataagg caacctatac
     4981 cgcctatacc aaaggcagtg ggcttactgc gaagctgtta atgcaaaact ggaatgaaga
     5041 tttgcatacc gctggattta tcatcgacgc caacccgcag tcagcgaaaa ttgcgacatt
     5101 atctgccagc aataatggtg tgctcgccaa tgagaatgca gcaaacaccg tctcggtcaa
     5161 tgtcgctgat gaaggaagca acccaatcaa tgatcatacc gtcacgtttg cggtattaag
     5221 cggatcggca acttccttta acaatcaaaa caccgcaaaa acggatgtta atggtctggc
     5281 gacttttgat ctgaaaagta gtaagcagga agacaacacg gttgaagtca cccttgaaaa
     5341 tggcgtgaaa caaacgttaa tcgtcagttt tgtcggcgac tcgagtaccg cgcaggttga
     5401 tctgcagaag tcgaaaaatg aagtggtcgc tgacggcaat gacagtgcca caatgaccgc
     5461 gacagttcgg gatgcaaaag gcaacctgct caatgacgtc aaggtcacct tcaatgtcaa
     5521 ttcagcagca gcgaaactga gccaaaccga agtgaatagc cacgacggga tcgccacagc
     5581 tacgctgacc agtttgaaaa atggtgatta tacggttacg gcctctgtga gctctggttc
     5641 tcaggctaat caacaggtga tttttatcgg tgatcaaagt actgctgccc tgaccctcag
     5701 tgtgccttca ggtgatatca ccgtcaccaa cacagctccg ctacatatga ctgcaacctt
     5761 gcaggataaa aatggcaatc cactaaaaga taaagaaatc accttctctg tgccaaacga
     5821 cgtcgcaagt cggttctcga ttagcaacag cggaaaaggc atgacggata gcaacgggac
     5881 tgcaatcgcc tccctgaccg gcacgttagc gggcacgcat atgatcacgg ctcgtctggc
     5941 taacagcaat gtcagcgata cacagccaat gacgtttgtg gcggataaag acagagcggt
     6001 tgtcgttctg caaacatcga aagcggaaat cattgggaat ggcgtggatg agacgactct
     6061 gacagcaaca gttaaagatc cttttgataa cgtggttaaa aatctttcag tagtcttccg
     6121 cacctccccc gcagacacgc aactgagtct gaacgcgcgt aatactaatg agaacggtat
     6181 tgccgaagtt acccttaagg gcacggtttt gggtgttcat acagccgaag ccatactgct
     6241 taacggcaac agagatacga aaatcgtcaa tattgcgccc gatgccagca acgcgcaggt
     6301 caccctgaac atccctgcac aacaggtggt gacgaataac agtgacagcg tgcagctgac
     6361 ggcgacggtg aaagacccgt cgaatcatcc ggtggcggga ataacggtga acttcaccat
     6421 gccacaggac gtggcggcaa actttaccct tgaaaataac ggtattgcca tcactcaggc
     6481 caatggcgaa gcgcatgtca ccctcaaagg caaaaaagcg ggcacgcata ctgtgaccgc
     6541 cacgctgggt aacaataatg ccagcgatgc gcaaccagtc accttcgtgg cggataagga
     6601 cagcgcggtt gtcgttctgc aaacatcgaa agcggaaatc attgggaatg gcgtggatga
     6661 gacgactctg acggcaacag tgaaagatcc ttttgataac gcagtaaaag atctacaggt
     6721 caccttcagt accaaccccg cagatactca acttagtcag agcaaaagca atactaacga
     6781 cagtggtgtg gccgaagtta cctttaaggg cacggttttg ggtgttcata cagccgaagc
     6841 cacactgcct aacggcaaca acgatacgaa gatagtcaat attgcgcccg atgccagcaa
     6901 cgcgcaggtt acgctgaaca tccctgctca acaggtggtg acgaataaca gcgacagcgt
     6961 gcagctgacg gcgacggtga aagatccgtc gaatcatccg gtggcgggaa taacggtgaa
     7021 cttcaccatg ccacaggacg tggcggcaaa ctttaccctc gaaaataacg gtattgccat
     7081 cacccaggcc aatggggaag cgcatgtcac gctcaaaggt aaaaaagcgg gtacgcatac
     7141 ggttaccgca acgctgagta ataacaatac cagtgattca cagccggtaa cgtttgtggc
     7201 ggacaaaacc tcggctctgg ttgttcttca gatatcaaaa aatgagatca caggtaatgg
     7261 cgtcgatagc gcaacgctaa ctgcaacggt caaagatcag ttcgacaatg aggtgaacaa
     7321 tcttccggta acattcagca cagcttcttc aggcctcacc ctgaccccag gggaaagtaa
     7381 taccaatgag tctggcatcg cgcaggccac tctcgcaggc gttgcctttg gtgagcagac
     7441 ggtcactgca tcactggcta ataatggtgc cagcgacaac aaaactgtgc attttattgg
     7501 cgacacagcg gcggcaaaaa ttatcgagtt gacgcctgtc ccagacagca taatcgcagg
     7561 taccccgcag aacagctccg gcagcgtcat caccgccaca gtcgttgata ataatggctt
     7621 tccggtgaaa ggtgtgactg tgaacttcac cagcaacgca gcgacagccg aaatgacgaa
     7681 tggcggtcaa gccgtgacga acgaacaggg taaggctacc gtcacttata ccaatacccg
     7741 ctcctcgata gaatcaggag cgagaccgga taccgttgag gccagtctgg aaaatggtag
     7801 ctccacgctt agcacatcaa ttaatgtcaa cgctgatgcg tctacggcac atctcacctt
     7861 gctacaggca ctttttgata cagtctccgc aggcgacact accaatctgt atattgaggt
     7921 gaaggataat tacggcaacg gagtacccca gcaggaggta accctcagcg tttcaccaag
     7981 tgaaggtgtg acccccagta ataacgctat atatacgacc aatcacgacg gcaattttta
     8041 cgcaagcttt accgctacaa aagccggggt ataccaagtg acggcaaccc tcgaaaatgg
     8101 cgattcgatg caacaaacag tgacctatgt gccgaacgta gcgaatgctg aaatctcgct
     8161 ggcagcctcg aaggatccgg taattgccaa caataacgat ctcacgacac taacagcaac
     8221 agtcgctgat acagagggca atgcgatagc caacagtgag gtaacattta ctctgccgga
     8281 agatgtgagg gcgaacttca cgctgggcga tggcggtaaa gtggttactg atactgaagg
     8341 caaagcgaaa gtcacgctga aaggtacaaa agcaggcgct catactgtta cagcatcgat
     8401 ggctggcggt aagagtgagc agttggtggt gaactttatt gcggatacac tcactgcgca
     8461 ggttaatctt aacgttaccg aggacaattt tatcgctaat aacgtcggga tgaccaggct
     8521 gcaggcaaca gtgactgatg gaaacggcaa cccgttagcc aatgaggcgg tgacattcac
     8581 gctaccggca gatgtgagcg caagctttac tctcggacaa ggcggttccg ccattactga
     8641 catcaacggc aaggctgaag ttacactgag cggtacaaaa tccggcacct accccgtgac
     8701 agttagcgtg aacaattatg gtgtcagtga tacgaaacag gtgactttga ttgccgatgc
     8761 tggtaccgca aaactagcct ccttaacctc tgtatactca ttcgtcgtca gcacgaccga
     8821 gggcgcgacc atgactgcaa gcgtcactga cgctaacggc aacccggtag aaggtataaa
     8881 agttaatttc cgcggaactt ccgtcacgct aagcagcacc agcgttgaaa cggatgatcg
     8941 gggtttcgct gaaattcttg tgacaagcac cgaggtcgga ctgaaaacag tttcagcctc
     9001 tctggcagat aaacctactg aagtcatctc gcgattactg aatgcaaaag cagatattaa
     9061 ttctgcaacg attaccagtc tggagatacc tgaaggtcag gtcatggtcg cacaagacgt
     9121 agcagttaaa gctcacgtca acgaccagtt tggcaatccg attcttaatg aatctgtaac
     9181 attcagtgca gaaccaccag agcacatgac catcagccaa aatattgtct ctactgatac
     9241 gcatggtata gccgaggtca ctatgacgcc cgaaagaaac ggttcgtata tggtgaaagc
     9301 atccctggcg aatggatcct cttatgagaa ggatctggtg gtaatcgatc aaaaactgac
     9361 actctcggcg tccagcccgc ttatcggtgt caattcccca acaggtgcaa ctctgacggc
     9421 aacgctaact tctgcaaatg gcactccagt ggagggtcag gtcatcaact ttagcgtaac
     9481 gccagaaggt gcgacgttaa gtggcggaaa agtgagaacc aactcttcag gtcaggctcc
     9541 agtcgttctg accagcaata aagtcggtac atatacggtg actgcatcgt tccataacgg
     9601 cgtaacaata cagacacaga caatcgtgaa agtcactggc aactcaagca ccgcccatgt
     9661 tgctagcttt atcgctgatc catcgactat agccgccacc aacagtgatt taagtacctt
     9721 aaaggcaacg gttgaggatg gcagtggtaa cctgatcgaa ggtctcactg tgtacttcgc
     9781 cttaaaaagc ggctctgcca cattaacgtc attaacagcg gtgacagatc aaaacggaat
     9841 cgcgacaaca agcgtgagag gagcgataac ggggagcgtc acggtaagcg cagtcacgac
     9901 cgctggtgga atgcaaacag tagatataac gctggtggca ggcccggcag acgcctcgca
     9961 gtccgtcctt aagaacaatc ggtcatcatt gaaaggagac tttaccgata gtgctgagct
    10021 acatcttgtt ctgcacgata tatcaggcaa tccgatcaaa gtttctgaag ggctggaatt
    10081 tgtgcagtca ggtaccaacg cgccctatgt gcaagttagt gcaattgact acagtaaaaa
    10141 tttctcaggc gagtacaaag ccactgttac aggcggcgga gagggtatcg caacgctgat
    10201 ccctgtattg aatggtgttc atcaagcggg tctgagtacc acaatacaat tcactcgcgc
    10261 agaagacaaa ataatgagcg gtacagtgtt agtcaatggt gctaacctac cgacaactac
    10321 attcccttcg caggggttca ctggggcgta ttatcagttg aataatgaca actttgcccc
    10381 aggaaaaacg gcggctgatt atgagttttc aagctctgcc tcctgggttg atgttgatgc
    10441 taccggtaaa gtgacattta aaaatgtcgg cagcaaatgg gagaggatta cggcgacgcc
    10501 aaaaacaggc ggccctagct atatatacga aatccgagtg aagagttggt gggtgaacgc
    10561 cggcgatgct ttcatgatat acagccttgc tgaaaatttt tgcagtagca atggctacac
    10621 acttcccctt ggagaccatt taaaccatag tcgttcccga ggcatcgggt cactgtacag
    10681 tgaatgggga gatatggggc attacacgac tgaagctggt tttcattcaa atatgtattg
    10741 gtcatcgagt cccgcaaact caaacgaaca atacgtagtt tccctggcaa caggtgatca
    10801 aagcgtattt gaaaagcttg ggtttgctta tgcgacatgt tataaaaacc tctgattttc
    10861 cgttataatt tcttaaagag tgctccattc ggggcactct tttttacatt ccctctatag
    10921 agcttgccct tctctcacag aaaatgaata aataaaaatg cgataccgca agaatcgcgt
    10981 tcgatgtgac tatgatcgta tccggtgagt acagctttta tccaaaggct ggttttctgt
    11041 gatgattgac cagcctcaga gtattgatga caccaccgtt cataacagat cggcattact
    11101 atgcataagt actgtctcat attctcgcca aaacactttt taatgccttt gcgaattcat
    11161 ccagacttat attgtcaatt ttttgcccaa cgacgactga ctccgaaaca tcggccattg
    11221 cgggccatcg cgttttgtaa tcagcataaa ttgcaacaac gggggtatta taggcacttg
    11281 ccatatgtaa tattgaggtg tcaggagtta ttgcgatata agcatctttt atgattgctg
    11341 ctgaacgttt aatcgagggt gataaggata aacggtagac attattataa caatccacta
    11401 ataccctggc tttatcttcc ccttttggtc catgaacgat cactattggt atatctgttt
    11461 cactttgaat tttttctatt agattttctg caatcgatag tgaaaatgta cgatcctgcg
    11521 agctaccttc taaattaagc gcaatgtaag atcctaacga tcgcatttcc tcgcgcacct
    11581 catcgagtac atcctcactt agtggcaatt catatattgg cctgactgct ggaaaacccg
    11641 cctcacgcat cataaatgcc catgtcatgg gtacgggagc ccggagtttc tgatccatcc
    11701 gggacgcgtt cttacacaca ggggaatagc acttcatggt taaaccaaca acctgaaaat
    11761 tcgtttttgc tttcaactga ctgataaata acatcgtttt caggttcttt ttacgcatcg
    11821 cctctataca aagatccggc gtaccgtatt gctgtgttat gttcttcgct aaatctttta
    11881 tttcttttaa tgttgcgtga tcctgcatag tcattgtgac taatgttaat ttagtctgtt
    11941 caagtttgag cgcattaaag acttctaaat taattgtcga cgtaacaatt aatagatgct
    12001 taattttatg caattcaagc gcccgaataa caggaaagat ggccatagca tcgccaatct
    12061 gatcgggaat atggatgaca acaaagtctg ttttttcaat attgaaatta taagctttat
    12121 aatcgtagta actaaatgca atacgtctca acaatgatac taaaaacata actaacctcg
    12181 cctccctact ggttataatg caatgcagtc tatcagaatc atcagggtgc aattttgtgc
    12241 atatgcggac ttttatgttt catatctcga acctgtgggg cctctgctta atccttaaac
    12301 aacaccagca actcctgcgc tttcatcttc cagcgaattt ttcatgttgc cgttaatcag
    12361 ccataaaatc atttgcagat gcgctctgtc gaggtagtct cataaggttc gtttatagat
    12421 cgacgcaatg tgagttacct tttccatact aattataaaa agacagtaca aacaggatca
    12481 ttatggactc cacgctcatc tccactcgtc ccgatgaaag gacggtttcg ttaagtcgcg
    12541 cccgacgagc tgcgttaggc agtttcgctg gtgccgtcgt cgactggtat gattttttac
    12601 tctatggcat taccgccgca ctggtgttta atcgcgagtt tttcccgcaa gtaagcccgg
    12661 ctatgggaac gctcgccgca tttgccacct ttggcgtcgg attccttttc cgtccgctcg
    12721 gcggtgtcat tttcggtcac tttggcgacc gactgggacg taagcgcatg ttaatgctga
    12781 ccgtctggat gatgggcatc gcgacagcct tgattggtat tcttccttca ttctcgacca
    12841 ttgggtggtg ggcacctatt ttactggtga cgctgcgtgc cattcaggga tttgccgtcg
    12901 gcggcgaatg gggaggcgcg gcgttgcttt ccgttgaaag tgcaccgaaa aataaaaaag
    12961 ccttttacag tagcggtgta caagttggct acggtgtagg tttactgctt tcaaccggac
    13021 tggtttcatt gatcagtatg atgacgactg acgaacagtt tttaagctgg ggctggcgca
    13081 ttcctttcct gtttagcatc gtactggtac tgggagcatt gtgggtgcgc aatggcatgg
    13141 aagagtccgc ggaatttgaa caacagcaat ataatcaagc ggccgcgaaa aaacgcatcc
    13201 cggttatcga agcgctgtta cgacatcccg gtgctttcct gaagattatt gcgctacgac
    13261 tgtgcgagtt gctgacaatg tacatcgtta ccgcctttgc acttaattat tcaactcaga
    13321 atatggggtt accgcgcgaa cttttcctta atattggttt gctggtaggt ggattaagct
    13381 gcctgacaat tccctgtttt gcctggcttg ccgatcgttt tggtcgccgc agggtttata
    13441 tcacaggcgc gttgatcgga acgttgagcg catttccttt ctttatggcg cttgaagcac
    13501 aatctatttt ctggatagtt ttcttctcca taatgctggc aaacattgcg catgacatgg
    13561 tggtgtgtgt gcaacaaccg atgtttaccg aaatgtttgg tgccagttat cgctatagtg
    13621 gtgctggagt cggttatcag gttgccagtg tggttggcgg tggatttaca ccttttattg
    13681 ccgctgcgct catcacttac tttgccggga actggcatag cgtcgccatt tatttgctgg
    13741 ctggatgtct gatttccgca atgaccgctt tgttgatgaa agacaatcaa cgcgcttgat
    13801 agtctggcga agatcatccg atcttcgcct tacacttttg tttcacattc ctgtgacata
    13861 ctatcggatg tgcggtaatt gtatggaaca ggagacacac atgaataata agggctccgg
    13921 tctgacccca gctcaggcac tggataaact cgacgcgctg tatgagcaat ctgtagtcgc
    13981 attacgcaac gccattggca actatattac aagtggcgaa ttacctgatg aaaacgcccg
    14041 caaacaaggt ctttttgtct atccatctct gaccgtaacc tgggacggta gcacaacaaa
    14101 tccccccaaa acgcgcgcat ttggtcgttt tactcacgca ggcagctaca ccaccacgat
    14161 tactcgccct actctctttc gttcgtatct taatgaacaa cttacgttgc tgtatcagga
    14221 ttatggcgcg catatctcgg tgcaaccctc acagcatgaa atcccttatc cttatgtcat
    14281 cgatggctct gaattaacac ttgatcgctc aatgagcgct gggttaactc gctatttccc
    14341 gacaacagaa ctggcgcaaa ttggcgatga aactgcagac ggcatttatc atccaactga
    14401 attctccccg ctatcgcatt ttgatgcgcg ccgcgtcgat ttttccctcg cgcggttgcg
    14461 ccattatacc ggtacgccag ttgaacattt tcagccgttc gtcttgttta ccaactacac
    14521 acgttatgtg gatgaattcg ttcgttgggg atgcagccag atcctcgatc ctgatagtcc
    14581 ctacattgcc ctttcttgtg ctggcgggaa ctggatcacc gccgaaaccg aagcgccaga
    14641 agaagccatt tccgaccttg catggaaaaa acaccagatg ccagcatggc atttaattac
    14701 cgccgatggt cagggtatta ctctggtgaa tattggcgtg ggaccgtcaa atgctaaaac
    14761 catctgcgat catctggcag tgctacgccc ggatgtctgg ttgatgattg gtcactgtgg
    14821 cggattacgt gaaagtcagg ccattggcga ttatgtactt gcacacgctt atttacgcga
    14881 tgaccacgtt cttgatgcgg ttctgccacc cgatattcct attccgagca ttgctgaagt
    14941 gcaacgtgcg ctttatgacg ccaccaagct ggtgagcggc aggcccggtg aggaagtcaa
    15001 acagcggcta cgtactggta ctgtggtaac cacagatgac aggaactggg aattacgtta
    15061 ctcagcttct gcacttcgtt ttaacttaag ccgggccgta gcaattgata tggaaagtgc
    15121 aaccattgcc gcgcaaggat atcgtttccg cgtgccatac gggacactac tgtgtgtttc
    15181 agataaaccg ttgcatggcg agattaaact tcccggtcag gctaaccgtt tttatgaagg
    15241 cgctatttcc gaacacctac aaattggcat tcgggcgatc gatttgctgc gcgcagaagg
    15301 cgaccgactg cattcacgta aattacgaac ctttaatgag ccgccgttcc gataataagt
    15361 tctgaacctt cttatcaaaa atagatgtga aggattaagt aagacatctg gcaaatagcc
    15421 cgcaataaac gcgggctgtt tgcttccttg ggcggatacg agttttatta tcgtcttaat
    15481 gatttccaca tattaaaagc aagtatgctt tcaaaacaca attataaaaa atcccgc
//
LOCUS       DAZFAB010000102        15356 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZFAB010000102 DAZFAB010000000
VERSION     DAZFAB010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.102"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..177
                     /gene="hemP"
                     /locus_tag="ACHJWA_003721"
     CDS             <1..177
                     /gene="hemP"
                     /locus_tag="ACHJWA_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFO4310237.1"
                     /translation="SRKLTPETDANHKTASPQPIRRISSQTLLGPDGKLIIDHDGQEY
                     LLRKTQAGKLLLTK"
     gene            complement(181..1617)
                     /gene="selO"
                     /locus_tag="ACHJWA_003722"
     CDS             complement(181..1617)
                     /gene="selO"
                     /locus_tag="ACHJWA_003722"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFO4310238.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPDKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLNDYEPGFICNHSD
                     HQGRYSFDNQPAVALWILQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(1680..2393)
                     /gene="ydiV"
                     /locus_tag="ACHJWA_003723"
     CDS             complement(1680..2393)
                     /gene="ydiV"
                     /locus_tag="ACHJWA_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFO4310239.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            complement(2640..3104)
                     /gene="nlpC"
                     /locus_tag="ACHJWA_003724"
     CDS             complement(2640..3104)
                     /gene="nlpC"
                     /locus_tag="ACHJWA_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFO4310240.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            complement(3182..3931)
                     /gene="btuD"
                     /locus_tag="ACHJWA_003725"
     CDS             complement(3182..3931)
                     /gene="btuD"
                     /locus_tag="ACHJWA_003725"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFO4310241.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     DVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            complement(3931..4482)
                     /gene="btuE"
                     /locus_tag="ACHJWA_003726"
     CDS             complement(3931..4482)
                     /gene="btuE"
                     /locus_tag="ACHJWA_003726"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFO4310242.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLISAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            complement(4545..5525)
                     /gene="btuC"
                     /locus_tag="ACHJWA_003727"
     CDS             complement(4545..5525)
                     /gene="btuC"
                     /locus_tag="ACHJWA_003727"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFO4310243.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     SPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPMLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRALL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            complement(5626..5925)
                     /gene="ihfA"
                     /locus_tag="ACHJWA_003728"
     CDS             complement(5626..5925)
                     /gene="ihfA"
                     /locus_tag="ACHJWA_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFO4310244.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            complement(5930..8317)
                     /gene="pheT"
                     /locus_tag="ACHJWA_003729"
     CDS             complement(5930..8317)
                     /gene="pheT"
                     /locus_tag="ACHJWA_003729"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFO4310245.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHNGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQYKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            complement(8332..9315)
                     /gene="pheS"
                     /locus_tag="ACHJWA_003730"
     CDS             complement(8332..9315)
                     /gene="pheS"
                     /locus_tag="ACHJWA_003730"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFO4310246.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     misc_feature    complement(9524..9648)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            complement(9598..9642)
                     /gene="pheM"
                     /locus_tag="ACHJWA_003731"
     CDS             complement(9598..9642)
                     /gene="pheM"
                     /locus_tag="ACHJWA_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFO4310247.1"
                     /translation="MNAAIFRFFFYFST"
     gene            complement(9765..10121)
                     /gene="rplT"
                     /locus_tag="ACHJWA_003732"
     CDS             complement(9765..10121)
                     /gene="rplT"
                     /locus_tag="ACHJWA_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFO4310248.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     gene            complement(10174..10371)
                     /gene="rpmI"
                     /locus_tag="ACHJWA_003733"
     CDS             complement(10174..10371)
                     /gene="rpmI"
                     /locus_tag="ACHJWA_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFO4310249.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            complement(10468..11010)
                     /gene="infC"
                     /locus_tag="ACHJWA_003734"
     CDS             complement(10468..11010)
                     /gene="infC"
                     /locus_tag="ACHJWA_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFO4310250.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            complement(11014..12942)
                     /gene="thrS"
                     /locus_tag="ACHJWA_003735"
     CDS             complement(11014..12942)
                     /gene="thrS"
                     /locus_tag="ACHJWA_003735"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFO4310251.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKIAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            13465..>15356
                     /gene="espL1"
                     /locus_tag="ACHJWA_003736"
     CDS             13465..>15356
                     /gene="espL1"
                     /locus_tag="ACHJWA_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HFO4310252.1"
                     /translation="MSQNDIIIRTHYKSPHRMHIDSDIPTPSSEPINQFAPQLITLLD
                     TSDLSSMLSYCVTQEFTANCRKISQNCYSTALFTINFATSPIHAENIFITLHYKKEII
                     SLLLETTPIKANHLRSILDYIEQEQLTAENRNHCMKLSKKIHREKTIQPTVNLNSSAF
                     FSQSPSDAIFCRHLSLQYALDSLRNGKGKVNLIKHYSSVESIQQHVPLVRDAEFRALL
                     RHPPAGSRVIASKDFGFALDIFFCRMMANNVSHMSAILYIDNHTLSVRLRIKQSAYGQ
                     LNYVVSVYDPNDTNVAVRGTHRTARGFLSLDKFISSGPDAQTWADRYVRNCAIAILPL
                     LPEGVPGAIFTGIATRMPFAPIHPSAMLLIMATGQTQQLITLFRQLPILPEKEIIEII
                     TAQNSIGTPALFLAMMNGHTDNVKIFMQEIQSLVDNHIIHEDNLVKLLQTKSANETPG
                     LYISMLYGFDEIIDIFLNALTTPIAQELLNKKLVMSILAMKIHDGEPGLYAAMENNHP
                     LCVTRFLSKINGIAFKYKLSKANIMDLLKGATAQGTPALYIAMSKGNEDVVLSYISTL
                     GAFAKKHSFSQHQLFTLLAAKNHDNMSAVHIAIHHNHYKTVETYYAAINVISQSMSFS
                     ADELKT"
BASE COUNT         3942 a         4014 c         3494 g         3906 t
ORIGIN      
        1 agcagaaaac tcacgcctga aacggatgcc aatcacaaga ccgcttcccc gcagcctatt
       61 cggcgaattt ccagccagac actgttaggt ccggatggca aactgattat cgatcatgac
      121 gggcaagaat atctgctccg taaaactcag gctggcaagc tgctgttgac caagtagcct
      181 ttaactcgag cagctgactt ccagccgttt accccagtca ggtggacgac tgacatagtc
      241 atcatctctg tcgctgaaag gatttcgcaa cgcctcatgc aggcggtgca attccgtcat
      301 atcacccttt tctgccgctt caatcgcccg ttgcgccaac cagttgcgca ataccagagc
      361 ggggttaacg ctttgcatca gttgctgacg ctcactatcg ctaacctcgt cttgttgcaa
      421 acgccctcga taacgggcaa accagtcatc aaatgccgca cgatcaataa actcatcacg
      481 tagcggtgac gccgcgctgt gctgctcggt cagactcagc atgcgaaatg tgcgggtata
      541 atcgctgcgc tctcgcgcca tcagactgaa taattcattc agtagcgcgt tatcctcttt
      601 ttgctccgtc ataaagccca gtttctgccg catccgttgt ccataatgcg tcaacaaaac
      661 ctgctgatag ctgtccaggg cctcattcag ggcatctacg gcaacaaatg gtgacaatgt
      721 ctgcgccaga cgctgtaaaa tccacaacgc gacagcaggt tgattgtcaa agctgtaacg
      781 tccttgatga tccgagtgat tacaaataaa accgggttcg taatcattaa gaaaaccaaa
      841 cggcccgtaa tcaagcgtca gtcccagcag cgacatgttg tcggtattca tcaccccatg
      901 tgcaaagccg accgtctgcc attgggcaat taacgatgcg gtacgtgcga caacatcgtt
      961 aaaccagaga cggtatttgt cctcatcatc ttcaagatgt gaccagtaat gacgaatggc
     1021 aaagtcagcc aactgacgaa ccttatccgg ctcgcggcga tagtaaaaat gttcgaaatg
     1081 accaaagcgc agatgacttg gtgccacacg catcagcatc gcgcctggct ccaccgtttc
     1141 ccgatacact ggcgaatcgc tggtgacgat acttaacgcg cgggtcgtcg gaatgcccag
     1201 ataatgcatc gcctcactgg cgagactttc tcgtatcgtc gaacgtaata ccgcccgtcc
     1261 atcacccatt cgcgaataag gcgtcaggcc agcacctttc agatgccagt ccattgtagt
     1321 gccatcagca agcagttgtt cgccaagtaa aatgccgcgc ccatcaccca gttggcccgc
     1381 ccagacgccg aactgatgac cactgtaaac ctgcgccagt ggtgacatac caggcagtag
     1441 ggtttcgccg ccccagacac ctgcgccatt tttaaacagc gacgatggaa tactcagcgt
     1501 gttagcgagt tcggtattat gccaaatcag ccgggcatta tttaaaggcg taggggaaag
     1561 tgctgtatag gtttctggca attcatcgcg ccagcgggta ataaaagaca gggtcataga
     1621 gcctcctgtt tgatagtgta gacggttact ctcgtcttaa acaccagcaa acaggagggt
     1681 tatcgctgaa ccaacgtcgt tatctgattt atgggaacgg caggccacag gcatccctgg
     1741 agagcatgga aatcgaaggg ggtaatctgt gctaatattt ctggcgtatc aatgcccccc
     1801 gcgatgatgc aattgcaaca aggggaaatt tgcgcctgaa tcgcacgaat aaacggttcg
     1861 aaggagcgat gcgtgatttg ttgctgaata aaacttttat ccagcattac gcgggtgaaa
     1921 agaccatcaa aaaccgcttt cattgtgctg ttgcctgctc ccaaattacc taacacaagg
     1981 ggatacactt gcgataagga taaaagaccg cgattatctt ttccttcatt gagatgcggg
     2041 taattctcat taatcaacag ttcaataaac ggatatttta atagttcgcc tgcgtaatta
     2101 tccctctcta ataataatgt agccacttgt ggcgttaaat taagccaggc aaagagttta
     2161 tgttgaataa aaaaatgttg gcatgatttt agtaattcca actgctctga aaataattgc
     2221 caatgttgct cctcggtaag ttgcgctata acccgactcg tcgggatacg caccgtgcca
     2281 tcttcactgg agaaatgggt aattaactca acgccgacaa ggacttgctg gttatctctt
     2341 atcggcagga aatagcaatc agaatgataa agattctcca aaaaaatctt catggtaacc
     2401 gtccctctcg aaggatgttt ttcagtatcc ggctaaaagg gatgaggcca taagacataa
     2461 caattacaga aggagtaact ttcatttgtt ccatgttaac cactttttca ggggtccttt
     2521 ttttagatta tcctgattat aaacgaataa tccatttacg gaatttttgt ctgcaaaata
     2581 ctactgtatt ctggagtaaa tggacggcga tgtatcatcg acgtccaatt ttcgacatac
     2641 tagattcgtc tcgcctgcca gaaatttttc tgccaataga cattatcaag tgaggaacgc
     2701 atcactccct tgctggtaga ggcgtggata aattggttgt tggtatcgta aatacctaca
     2761 tgcaaaccat tttgtccgga acccgttttg aaaaagacca ggtcaccagg cagcaactcg
     2821 tctttatcaa tttgcgtgcc gatagaggct tgttctttgg tttctcgggg cagctgcaaa
     2881 tcgaaacgat cgcgcatcgt cacaaccaca aatcccgaac agtccacacc gcgccgcgtc
     2941 atgccaccat aacgatacgg cgtgccatgc cagctttgta gctggtcgtt caaaccggca
     3001 ataacggtaa tcgaatcaga aagtctggca tttggcggcg gtgctttatg gtggctacac
     3061 ccggccagaa acagtgctgt gatcaaaata aggcagaaac gcattccgta cggttcctct
     3121 gttttttatt cttgcattaa tttagcgtcg taattacccg attttcaaga tactaatgaa
     3181 atcagatggt cgaaatcagc attctgtgac cttcgatatc cagacggcga aaattcatcc
     3241 cataggcctg cgccagattt ggcggcgtga gcacatcttc tctgcgtcca ctggccagca
     3301 tttttccacc ttttagcaac cacgcccgat gcgcatgacg caatgtgtgg ttgagatcgt
     3361 gactgctcat cacaatcgcc agtccttgct gacacagcgc gctcagaatt ttgtctaacg
     3421 cactttgttg cgcaacatca agactgttca tcggctcatc aagaagcagc aattggcctg
     3481 cgggattggc ttgtggtgtg atttgcagca ccaccgcagc aagacgtacg cgttgccatt
     3541 caccgccgga aagttgattg gtgctacgac cgagtttgtc atcaagagcc agcgcccctg
     3601 cgacatcatt cagtagttcg gtacgcgttt tatcgtgctg atgcagtgtc aggtagtgcc
     3661 agaccggcat tgcaaacggc ggcgtctgct gttgtgaaag ataggcacga tgcagcgcga
     3721 gttttgttgc ggaccatgct tccagtggtt gccccgcgaa ctgaatgctt cccttaccgc
     3781 tggtcattcc ggccattcgc gccagtaagg tactcttacc cgcgccattc ggccccacca
     3841 ggtgcaggat ctccccagcc cgaacctcgc cagaaagcgg ccccaggcgg gtagattccg
     3901 caacatcctg taactgcatc acaatagaca ttattttgcc aacgccagtt taatgctttc
     3961 catcacaatg ggatcttccg gcgtcatatc cggggaaaaa cgctggatga ccaatccgtc
     4021 cctgccaact aaaaatttct caaaattcca taaaatatca tcagggtaca gcggtgcacg
     4081 acctttgctg accatacggg catagaatcc gctctcttcc ggcgcgactg cggtcggcgc
     4141 tgcggaaatc aatttttgat acagcggatg gcgtccttcg ccattaactt caatcttact
     4201 gaacatcggg aacgtcaccc cccatgtggt ggtacagtaa gttttaatct cttcatcgct
     4261 gcccggttct tgttccagaa actggttgca cgggaatccc agcaccacaa aacctcgatc
     4321 ggcccaggct ttctgaatat tctccaactg ctcatattgc ggcgttaagc cacactttga
     4381 ggcgacattg acaatcaaca gcacattacc ggcgtacttc tccagcgtgg tcacttcacc
     4441 gtcgatatct ttcactacgg tcgtcagaat ggaatcttgc atcgtttctc ctgggtgtgg
     4501 tcagtaaaaa tcttagcttt taatcataga ccgtcttttt gcggctaacg tcctgctttt
     4561 aacaataacc agataaacac cggcgcaccc aacgttgcgg tgaccacgcc aataggcagc
     4621 tctgcggcag ctaatgccag gcgcgctaca atatcggcca gcagcaatgc gctcgcccct
     4681 gccagcgcgc agccgggaag taatgcgcga tgatcggtta aaccacacaa ccgaagaata
     4741 tgtgggatca ccagaccaat aaagccgata gcacccgcca gcgctacact gacgccaacc
     4801 atccagccgg tcgctgccac cagcacattg cgccagaacc acaggggtaa acccagttgc
     4861 cgcgccgaga tctcgccaag tgctaacata ttcatcggcc tggactgaca acagatccac
     4921 aacaacatgg ggatcaatgc cagcatcagc cagctttgcc gccagtctac gccgccaaaa
     4981 ccgcccatca tccagtacat cagctgacgt aaatcaacag aggtggaaaa gtagatagcc
     5041 cacgtcatta gtgcgctaca gataatccct aatgcaacgc cagccagcaa taaccgactg
     5101 gtcgaaagat gacgacgggc gaaacgtaag agtattaaag tgatgataag cgccccagca
     5161 atcgcacaca gccctagcgc ccagttgggg agttgccctt gcccaagcaa taccgcggcg
     5221 ataagcccca cgcctgcgcc gttagagacg ccaagtagtc caggttctgc cagaggattt
     5281 tcaaacaacg cctgcattac agcgccggat atagccagcg ccgcaccaac cagcaataca
     5341 gccagcgtac gtggcaggcg aatttgccag acgaacagtt cgccacgagg agaaaaccag
     5401 tcacctggcg agatccattg ttcaccggcg caaaggctta agagaagcgc cagcagcatc
     5461 aaaactgaca ggcataataa ccagcgaata ttttgtcgct gttgttggcg ggcaagtgtc
     5521 agcatggtat ccgttctgct gaagtgtcat ggcgttgatt ttacggtgac tcttcgacag
     5581 tgaaaagaaa aaaggccgca gagcggcctt tttagttaga tcagattact cgtctttggg
     5641 cgaagcgttt tcgacccggc tttttaactt ctgcccgggt ctgaaggtca ccacgcgccg
     5701 tgctgtaatg ggaatatcct cgcccgtttt cgggttacgt cccgggcgtt gattcttatc
     5761 acgcagatcg aagttaccaa aaccagagag tttcacctgt tcgccgtttt ccagagcgcg
     5821 acggatctct tcgaaaaaca gttcaaccag ttctttggca tcccgcttgc taagcccaag
     5881 cttatcaaac agatattctg acatttcagc ttttgtaagc gccataggtt caatccctca
     5941 atgatgcctg gaatcgctct tttaatgcct ctacacattt ggcgacggta gcggcaatct
     6001 cctcttcttc gagtgtacgg ctggtatctt gcaggatcag gcttatggcg aggctcttat
     6061 acccctccgc aacaccctta ccgcggtaca cgtcaaataa gtttacgcca actacctgat
     6121 ttacgccaac tttcttacat tcggataaaa tatccgctgc gggaacgttt tctgcgacca
     6181 ccaccgcgat gtcacgacgg ttcgccggga agcgagaaat ctcgcgcgcc tgaggcacca
     6241 cgcggtctgc gagcttgttc cactccagtt cgaacaccag agtgcgaccg ttaagatcca
     6301 gtttacgttc cagttcagga tgaacaaccc caacaaaacc aatacgttca cctttcagat
     6361 aaatcgctgc ggattgcccc ggatgcagtg ccggattcgc ttctgcacgg aactcaacct
     6421 cattcagttt accggtcagg tcgagaacgg attcaagatc gcctttcaaa tcatagaaat
     6481 caacggtctc ttttgccagg ttccagtgct cttcgtaacg gttaccgcaa atcacaccgg
     6541 ctaacatcag atcctgacga atgcccaacg gtgcctgagt atctggtacg aaacgcagac
     6601 cgctttcgaa aatgcgcaca cggttctgct gacggttctg gttgtacacc acggttgcca
     6661 gcaggccggt ccacagagaa agacgcattg ctgacatttc aacagagatc gggcttggca
     6721 gcagtaaggc ttcaacgcct ggatggatca tctgctgcac tttcggatca acgaagctat
     6781 aggtgatcac ttcctgatag cctttatcgt tgagcagcgt tttcacgcgc ttgagcgaca
     6841 ggtcagcttc acggtgagta cccataatca ggcttgcctg taccggctca tccgggatgt
     6901 tgttgtagcc gtaaacacgc gcgacttctt cgaccagatc ttcttcaatc tccatgtcga
     6961 aacgccagct cggcgcaact gcctgccact cgtctttgcc ttcggtcact tcgcagccga
     7021 gacgacgcag aatgtcagtt acctgctcat ccgcaatatg atggccgatc aggcgatcca
     7081 gtttgctacg acgtaaagtg atggttgcac gcttcggcag cgttgcttcg ttggtgatat
     7141 caattaccgg accagcctca ccaccgcaga tgtcaatcag cagacgggtc gcacgttcca
     7201 tcgctttgta ctgcagtgcc ggatcaacgc cacgctcata acggtgagac gcatcagtat
     7261 gcaggccatg acgacgagca cgaccggtga tagacagcgg gctaaagaaa gcgcattcca
     7321 gcagcacgtt ttgtgtttcg tcattcacgc cagagtgttc gccaccgaag atgccgccca
     7381 tcgccagcgc cttgttgtgg tcggcgatga ccagagtgtc agcattcagc ttcgcttcag
     7441 taccgtcgag cagcaccagc gtttcgccct ctttcgccat ccgcaccaca atgccgcctt
     7501 caatgcgatc tttatcgaaa gcgtgcatcg gctggcccaa ttcgagcagc acatagttgg
     7561 tgacgtcaac aactgcatcg atagaacgga tcccgcaacg acgcagtttt tctttcatcc
     7621 acagcggagt tggcgcttta acgttaatgc cttttaccac acggccaaga taacgcgggc
     7681 aggcttccgg cgcttcgact gtaatcggca gcgtgtcgtc gatggtcgca ccaaccggaa
     7741 cgatttccgg ttcaaccagc ggcagctgat tcagcacggc aacgtcacgc gcaacaccaa
     7801 tgatacctaa acagtcggca cggtttggcg ttacgctgat ttcgatggtg ttgtcatcga
     7861 gtttcaggta ttcgcggatg tcagtgccaa tcggcgcatc tgcaggcagt tcgataatgc
     7921 cgttatggtc gtcagaaatt cccagctcag aaaaggagca cagcatccct tcagacggtt
     7981 cgccgcgcag tttcgccgct ttaattttga aatcacccgg cagaacagca ccaatggtcg
     8041 ctaccgccac acgcaggccc tgacggcagt ttggcgcacc gcagacgatg tccagcaggc
     8101 gatcgccgcc gacattcact tttgtcacac gcagtttgtc agcgttcgga tgctgcgcac
     8161 actcaaccac ttcaccaacg accacgccgt ggaagctacc ggcaaccggt tctacaccgt
     8221 caacttccag gcccgccata gtgatttgat tcgccagcgc atcgctatca atcgccgggt
     8281 tcacccattc gcgtaaccac agttcactga atttcataat ctattcctgc cttatttaaa
     8341 ctgtttgagg aaacgcagat cgttttcgaa gaatgaacgc aggtcggtga cgccgtaacg
     8401 caacatagtc agacgctcca tccccatccc gaaggcgaaa ccagagtaaa cttccgggtc
     8461 gatgccaacg ttacgcagca cgttcggatg caccatcccg cagcccagta cttccagcca
     8521 tttaccgttt ttacccatga catccacttc tgcagaaggt tcggtaaacg ggaagtagga
     8581 aggacggaag cgaatctgca aatcttcctc aaagaagtta cgcaggaagt cgtgcagcgt
     8641 gcctttcagg ttggtaaagc tgatgttggt atcaacaatc agaccttcca tctgatggaa
     8701 catcggcgtg tgagtctggt cgtagtcgtt acgataaaca cggccaggcg cgatgatacg
     8761 aatcggtggc tgctgggctt tcatggtgcg gatctgtacg ccagaggtct gggtacgcag
     8821 caggcgggta gcgtcaaacc agaaagtgtc gtggtcagcg cgcgccgggt ggtgaccagg
     8881 aatgttcaga gcatcgaagt tatgataatc gtcttcgatt tccggcccgg ttgccacggt
     8941 aaagccaagc tcaccgaaga aactttcgat acggtcgatg gtacgggtaa ccggatgcag
     9001 accgccgttt tcaatgcgac gacctggcag agagacatca atcgtttccg ccgccagacg
     9061 cgcattcagt gcagcgcttt ccagttccgc tttacgcgca ttcagcgcct gctgaacctg
     9121 ctctttcgct tcgttgataa ccgcaccggc tgccggacgc tcttctggcg gcagctcacg
     9181 cagggtcgtc atctgaaggg ttaagtgccc ttttttaccc aaatattcga cgcgcacatt
     9241 gtctaacgcg gcaacatctg acgcctggct aatggccgcc ttcgcactgg caaccagttc
     9301 tgcgagatgt gacatggttt tcctcattgt gtcagtggtg acactggttc gttggactta
     9361 gagcctatcc catcaggcta ttttacttgc cattttggtc ccgggcagtg ctcgaaatcc
     9421 tcacgtactt aagtacgctc cggtttctcc gcactggccg tatccagact ggctgcgaca
     9481 attacgcctg atagactggc tttttatttt tcaaaacgcg catacaaaaa aagcctccac
     9541 tgggaggctt tcaggcgctg ttttccgttt ctcttctcac gcgctagcct cctggattca
     9601 ggtgctaaag taaaaaaaga agcggaaaat agcagcattc attgcttgcg ttaccttttg
     9661 gtactcttca aaagaccttt attgaaaagg ctacggcgat aaaagtcaat gttttgatgg
     9721 cgttgaaacg aaaagaggga gactagctcc ctctttcaac tggcttatgc cagagctgct
     9781 ttcgcttttt caaccagagc ggtgaacgct actttgtcga atactgcgat atcagccagg
     9841 atcttacggt cgatttcaac agaggctttt ttcaggccat tgatgaattt gctgtaagaa
     9901 ataccgttct gacgtgctgc tgcgttgata cgcgcaatcc acagttgacg gaactgacgc
     9961 ttacgttgac gacggtcacg gtaagcatac tgaccagctt tgataacagc ctggaaggca
    10021 acgcggtata cgcgagaacg cgcaccgtag tagcctttag cttgtttcaa aattttcttg
    10081 tgacgtgcgc gtgcaataac accacgtttt acgcgagcca tatgtgctct cctgtatcta
    10141 tattctaatt aaaaagttaa aaacgttaac ggcttatgcg tacggcaggc acgcgattac
    10201 caggcccaga tcgcctttgg aaaccatggc tttcggacgc aggtgacgtt tacgtttggt
    10261 cgcttttttg gtcagaatgt gacgcaggtt agcgtgcttg tgcttaaaac cacctttacc
    10321 ggtttttttg aagcgcttag cagcaccgcg tacggtctta atttttggca ttttaataac
    10381 ttccacttcg cattgttaat aaacgaaaca aaggcgaaca aagcctgtga agcccgaagg
    10441 ctccacagac agtgctactt gaaggcctta ctgtttcttc ttaggagcga gcaccatgat
    10501 catctggcgg ccttcgatct tcgttgggaa ggattcgacc actgccagtt cttgcaaatc
    10561 gtctttcacg cgattaagca cttccatacc gatttgctgg tgcgccatct cacgaccgcg
    10621 gaaacgcagc gtgattttgg ctttatcacc ctcttcgaga aagcgaatca ggctgcggag
    10681 ttttacctga tagtcgcctt catctgtacc aggacggaat ttaatttcct taacctggat
    10741 aactttttgc tttttcttct gttccttaga agacttgctc ttttcataga ggaatttgcc
    10801 gtaatccatt atacgacaaa ccggcggctc ggcgttaggg ctgatctcga ctaagtctac
    10861 tccggcttct tctgctttct ccagagcttc tctcagactc acaataccaa gctgctcgcc
    10921 ttccagacct gttaagcgaa cttcctgggc gcgaatttcg ccattgatac ggttagggcg
    10981 cgccgtttga actcgttttc cgcctttaat accttattcc tccaattgtt taagactgcg
    11041 gctgcgaatc tcttgttgca gcttctcgat cacttcattt acgtccatgc ttcccaggtc
    11101 tttaccacgg cgggtgcgaa cggcaatttt gcctgattcc acctctttat caccacagac
    11161 cagcatatac gggacgcgac gcaaagtgtg ctcgcggatt ttaaagccaa tcttctcatt
    11221 tctcaagtct gctttaacac gaatgcccgc atttgatagt ttttgcgtca attcgttaac
    11281 gtattcagac tgtgaatcgg taatattcat gataacaacc tgaactggcg caagccaggt
    11341 cgggaagaaa ccagcgaatt cttcggtcag gataccgatg aaacgttcca tcgaccccag
    11401 aattgcgcgg tgaatcatta ccggtacctt acgctcgttg tcttcgccca cataggaggc
    11461 gctcagacga gacggcaagg agaagtccag ctgtactgta ccgcactgcc atgcacgatc
    11521 gaggcagtca tacagggtaa attcaatttt cggaccgtag aaagcgcctt cacccagttg
    11581 atattcaaac gggatgttgt tttcttccag cgcaaccgcc aggtccgcct cagcacgatc
    11641 ccacatttcg tcgctgccaa tacgcttttc aggacgagtg gagagtttga cgacgatctt
    11701 ctcgaagcca aaagtgctgt acatatcata gactaaacgg atacatccgt taacttcatc
    11761 gcgaatttgt tcttcagtac agaagatatg cgcgtcatcc tgggtgaatc cacgcacgcg
    11821 catcaggcca tgcagagaac ctgacggctc gttacggtgg cagctaccaa actcggccat
    11881 acgcagcggc agatcgcgat aagacttcag cccctggttg aaaatttgta cgtgacccgg
    11941 gcagttcatc ggcttaatgc agtattcacg gttctcagaa gacgtggtga acattgcatc
    12001 tttgtagttg tcccagtgac cggttttttc ccacaggaca cggtccatca tgaacggacc
    12061 tttaacttcc tgatactggt actctttcag tttagaacga acaaacactt ccagttcacg
    12121 gaagatggtc cagccgtcgt tgtgccagaa taccataccc ggcgcttctt cctgcatatg
    12181 gtacaggtcg agctgtttac cgattttacg gtggtcgcgt ttcgcggctt cttccaggcg
    12241 ctgcaggtaa gcgttaagcg cttttttgtc tgcccacgcc gtaccgtaaa tacgttgcaa
    12301 cattttgttg ttgctgtcgc cacgccagta agcccctgcc gttttcatta gtttgaaatg
    12361 atggcagaaa cgcatgttcg gtacgtgcgg accgcggcac atatcgacat attcttcatg
    12421 gaagtacaga cctggcttgt catcatgggc gatgttttcg tcaagaatgg agactttgta
    12481 gctctcccca cggttggcga aagtttcacg cgcttcgtgc cagctgactt tcttcttaat
    12541 gacgtcgtag tttttctcag caagctcatg catccgcttc tcgagtgctt cgacatcttc
    12601 ctgggttaac gtgcggtcaa gatcaacgtc gtaataaaaa ccgttgtcaa taaccgggcc
    12661 gattgccatt ttggtatgcg gccaaagttg tttaatcgcg tgccctaaca ggtgcgcaca
    12721 ggagtgacga atgatctcca gaccttcttc gtctttggcg gtaatgatcg acagttgtgc
    12781 gtcgttttca atcagatcgc aagcatcaac cagttcgcca ttaacgcgcc ctgcgataca
    12841 ggctttcgcc agacctggac caatgtccag cgcaacatcc atggggctta cagcgtggtc
    12901 gtaatggcgt tgactgccat caggaagagt tataacaggc attttatatc cttatttgca
    12961 gtggtgaccc acacgaaaga tcacatacaa agaaaaattt gtttattaac agttcattgc
    13021 gaaaccatct agccaacaaa tgctaaattg gttcgcaatc aggtacacaa atgaaagaca
    13081 ttaacctctc accttccacc cggttgagcg atgttaacac taaaaaaagg gagattgtac
    13141 ctttccgttt cacattgatt cgattcgcaa ttcgtttgta gtgagtacac cagcaaaacg
    13201 cttaaagaac aactgataac atcacggagc gtctgaaagg tttactgcga gcgaatttac
    13261 agcagcataa actgaatcca gcaaagttcg ttgcgtgatt tggcggtact atttaaacga
    13321 tcataagatt aatgacgaag tggtcatatc acaatgataa aagtgacaca attcttatag
    13381 caatttttcg tgcacatttc gttctggcga taataattaa tcatcatact caaaacctcc
    13441 agacatatat aaggcgaata gattatgagt caaaacgaca ttattatcag aactcattat
    13501 aagtctcctc atagaatgca catcgatagc gacataccaa cgccttcatc agagcctatt
    13561 aatcaatttg cgccccaact catcacccta cttgatacct ctgacttaag ttcgatgctg
    13621 tcatactgtg ttactcagga atttaccgca aactgtcgaa aaatatcaca aaattgttat
    13681 tccactgccc tttttaccat taactttgcc acttcaccca tccatgcaga aaatatattc
    13741 attacattac actataaaaa agaaatcatt tccttattac tggaaaccac gcctattaaa
    13801 gctaaccatt tgcgaagcat actggattat attgaacagg aacagttaac tgccgaaaat
    13861 cgtaaccatt gtatgaaact gtctaaaaaa atccatagag aaaaaactat acaaccaaca
    13921 gtaaatctca atagtagtgc atttttttcg caatctcctt ctgacgctat tttttgtcgc
    13981 catctgtcat tgcaatacgc acttgattca ttgagaaatg gaaaaggcaa ggtcaacctg
    14041 attaaacatt actcctccgt tgaatccata cagcagcatg tccccttagt ccgggacgcg
    14101 gagttcagag cattacttcg ccatcctcct gcagggagtc gcgttatcgc gagtaaggat
    14161 tttggcttcg ctttagatat tttcttctgt cgaatgatgg caaacaatgt cagtcatatg
    14221 tctgcgattt tatatataga caatcatact ttgtcagtaa ggctacgaat aaagcagtcg
    14281 gcgtatgggc aattaaatta tgttgtgtcc gtttacgacc cgaacgatac caacgttgcc
    14341 gtcagaggca cccacaggac agcacggggc tttctctcgc ttgataagtt catcagttca
    14401 ggtcccgatg ctcagacctg ggctgatagg tatgttcgca actgtgcaat tgctattctg
    14461 cccctattac ctgagggagt tccaggggct attttcacgg gtatcgcgac acgaatgcca
    14521 tttgccccta tacatccatc ggcaatgttg ttaataatgg ccacaggcca gactcaacag
    14581 cttattacat tattcagaca gttgcccata ctccctgaaa aagaaatcat tgaaataata
    14641 actgcgcaga atagcattgg tacacctgct ttatttttgg ctatgatgaa cggacatact
    14701 gacaacgtga aaatatttat gcaagaaatt cagtcactgg tagataatca catcattcat
    14761 gaagataatc tggttaaatt gttgcaaact aaaagtgcta acgaaacacc tggactttat
    14821 atctccatgt tgtatggatt cgatgagata atcgatatct ttctgaatgc attaaccact
    14881 cctatagcac aagagctttt aaacaaaaaa ctggtgatga gtattttagc catgaaaata
    14941 catgatggtg agccaggatt atacgccgca atggaaaata atcacccttt gtgtgtcaca
    15001 cggttcctct ctaaaattaa tggcatcgcc tttaaataca agttgagcaa agctaacatc
    15061 atggatttat taaaaggcgc tacagcacag ggaacccctg cattatacat cgctatgagc
    15121 aagggtaatg aagacgtcgt gttatcttat atatcgacgc tgggtgcttt tgcaaaaaaa
    15181 cattctttta gtcaacatca gttatttaca ctgttggctg ctaaaaatca tgacaacatg
    15241 tcagctgttc atatagccat tcatcataat cattataaaa ctgtagaaac atattatgct
    15301 gctataaatg taatcagcca aagcatgagt tttagtgctg atgaattaaa gacgta
//
LOCUS       DAZFAB010000103        14974 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZFAB010000103 DAZFAB010000000
VERSION     DAZFAB010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14974
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.103"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            140..382
                     /gene="yjbE"
                     /locus_tag="ACHJWA_003737"
     CDS             140..382
                     /gene="yjbE"
                     /locus_tag="ACHJWA_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFO4310253.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            496..1134
                     /gene="yjbF"
                     /locus_tag="ACHJWA_003738"
     CDS             496..1134
                     /gene="yjbF"
                     /locus_tag="ACHJWA_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFO4310254.1"
                     /translation="MKRPAFILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIIDGATWMRTMGWTEYQQVRYATARSVFKWDGTGTVKVGSDET
                     AVRVLDEEVSTDQARWHNRYWIDSGGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1131..1868
                     /gene="yjbG"
                     /locus_tag="ACHJWA_003739"
     CDS             1131..1868
                     /gene="yjbG"
                     /locus_tag="ACHJWA_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFO4310255.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERANPPLQGNYTLWVGPPPSTVTLFGLISRPGNQP
                     FTPGRDVASYLSGQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSEMPDALNADILQTLTQRIPQ"
     gene            1868..3964
                     /gene="yjbH"
                     /locus_tag="ACHJWA_003740"
     CDS             1868..3964
                     /gene="yjbH"
                     /locus_tag="ACHJWA_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO4310256.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERANTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIVRANRIVMNDLPDGIKTIRVTENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(4011..4289)
                     /locus_tag="ACHJWA_003741"
     CDS             complement(4011..4289)
                     /locus_tag="ACHJWA_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310257.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSALPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4290..4433
                     /locus_tag="ACHJWA_003742"
     CDS             4290..4433
                     /locus_tag="ACHJWA_003742"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310258.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            4559..4969
                     /gene="psiE"
                     /locus_tag="ACHJWA_003743"
     CDS             4559..4969
                     /gene="psiE"
                     /locus_tag="ACHJWA_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFO4310259.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQASKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVVTLWLCNSKRLKRE"
     gene            complement(5013..6488)
                     /gene="xylE"
                     /locus_tag="ACHJWA_003744"
     CDS             complement(5013..6488)
                     /gene="xylE"
                     /locus_tag="ACHJWA_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFO4310260.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPVYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAEGILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(6860..7750)
                     /gene="malG"
                     /locus_tag="ACHJWA_003745"
     CDS             complement(6860..7750)
                     /gene="malG"
                     /locus_tag="ACHJWA_003745"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFO4310261.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(7765..9309)
                     /gene="malF"
                     /locus_tag="ACHJWA_003746"
     CDS             complement(7765..9309)
                     /gene="malF"
                     /locus_tag="ACHJWA_003746"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFO4310262.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGREQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(9463..10653)
                     /gene="malE"
                     /locus_tag="ACHJWA_003747"
     CDS             complement(9463..10653)
                     /gene="malE"
                     /locus_tag="ACHJWA_003747"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFO4310263.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            11018..12133
                     /gene="malK"
                     /locus_tag="ACHJWA_003748"
     CDS             11018..12133
                     /gene="malK"
                     /locus_tag="ACHJWA_003748"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFO4310264.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            12205..13545
                     /gene="lamB"
                     /locus_tag="ACHJWA_003749"
     CDS             12205..13545
                     /gene="lamB"
                     /locus_tag="ACHJWA_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFO4310265.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEVNPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            13696..14616
                     /gene="malM"
                     /locus_tag="ACHJWA_003750"
     CDS             13696..14616
                     /gene="malM"
                     /locus_tag="ACHJWA_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFO4310266.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            14845..>14974
                     /locus_tag="ACHJWA_003751"
     CDS             14845..>14974
                     /locus_tag="ACHJWA_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310267.1"
                     /translation="MLGHISKFDGNNSLIKHGVVQGNNIVDFDLLRNFNGGPGLNRE"
BASE COUNT         3697 a         3844 c         3948 g         3485 t
ORIGIN      
        1 tttgatattc attccaatta tcctaaaacc ccatctctaa ttccccgcgc cgtaattcgc
       61 atgctttagt tgtgtatact cgatcccgcc cgaaatgttt ttgggtaaat ctccattcat
      121 tcaatgaagg gaaattgtta tgaaaaaagt tctgtatggc atttttgcca tatctgcgct
      181 tgcggcgact tctgcgtggg ctgcacctgt acaggtgggc gaagcggcag ggtcggcagc
      241 aacgtcggtt tcggcgggga gttcctccgc gaccagcgtc agcaccgtaa gctcggcggt
      301 gggtgtcgcg ctcgcggcaa ccggtggcgg tgatggttct aataccggga ccaccacaac
      361 cacgaccacc agtacccagt aataaagtat gtatccccaa aataattcga gtcattgcat
      421 ctgtggctag aagtatgaag ggatttacca taaccacact ccggtgtggt tattctgccc
      481 ctctggagaa gagtcgtgaa gcgacctgca ttcattctta tctgcctgct attacaggcc
      541 tgttcagcca cgactaaaga gctgggcaat tcactgtggg acagtctgtt cggcacgcca
      601 ggcgtacagc tgacggacga tgatattcaa aatatgccct acgccagcca gtacatgcag
      661 cttaatggtg ggccgcagtt gtttgtggtg ctggccttcg ctgaagacgg acaacaaaaa
      721 tgggtcactc aggatcaggc cactctcgtc acacaacatg gtcgtctggt gaagactttg
      781 cttggcggcg acaacctgat tgaagtgaat aaccttgccg ccgacccgct gattaaaccc
      841 gcacaaatca ttgatggcgc aacctggatg cgcacgatgg gctggaccga gtaccagcag
      901 gtacgctacg ccaccgcacg ctcagtcttc aaatgggatg gcaccggtac cgttaaggtc
      961 ggcagcgatg aaaccgcggt tcgcgtgctg gacgaagaag tctccaccga ccaggcgcgc
     1021 tggcataacc gctattggat cgacagcgga gggcaaattc gccagtcgga acagtatctc
     1081 ggcgcggatt atttcccggt gaaaaccact ctcatcaagg cggcaaaaca atgattaaac
     1141 aaactattgt cgcgttgctt ttgagtgtgg gagcgtcatc ggtcttcgcg gcaggaaccg
     1201 tcaaggtgtt cagcaatggc agcagtgagg ccaaaacgct aacgggcgca gagcatttaa
     1261 tcgatctggt aggccaaccg cggctggcaa acagctggtg gcctggtgcg gtgattagcg
     1321 aagagctggc aacggcggca gcattgcgtc agcagcaggc gttgctgaca cggctggcag
     1381 aacagggcgc agattccagc gccgacgatg ccgctgcgat taacgcctta cgccagcaaa
     1441 ttcaggcgtt gaaggtgacg ggcaggcaaa aaatcaatct tgatcccgat atcgtccgcg
     1501 ttgctgaacg cgctaacccg ccgttgcagg gcaactacac gctgtgggtc ggaccaccac
     1561 cgtccacggt cacgttgttc gggcttatca gccgtcctgg caatcagcca ttcactcccg
     1621 gtcgcgacgt ggcgagctat ctctccgggc aaagtctgct tagcggtgcg gatcgtagct
     1681 acgcgtgggt ggtttacccg gacggacgca cacaaaaagc gccggtggct tactggaaca
     1741 agcgtcacgt agagccgatg cccggcagca ttatttatgt tggcctcgcg gactccgtct
     1801 ggagtgagat gcctgatgcc cttaacgccg acattcttca gactctgacg cagcggatac
     1861 ctcaataatg aaaaaaagac atctgcttag cttactggcg ctgggcatta gcacagcttg
     1921 ctacggcgaa acatatcctg cgcccattgg tccgtcgcag tcggatttcg gtggcgtagg
     1981 attattacaa acgcccacgg ctcgcatggc gcgggaaggg gagttgagtc tgaactatcg
     2041 cgataacgat cagtaccgtt attactcagc ttcagtgcaa ctcttcccgt ggctggaaac
     2101 aacgctgcgc tacaccgacg tgcgcacccg gcagtacagc agcgtcgaag cgttctctgg
     2161 cgatcaaacg tataaagata aagccttcga tctcaaactg cgtttgtggg aagagagtta
     2221 ctggctgccg caagtggcgg ttggtgcgcg ggatatcggc ggtacggggc tgtttgatgc
     2281 ggaatatctt gttgccagca aagcctgggg gccgttcgat tttacgcttg gcctgggctg
     2341 gggatatttg ggcacaagcg gtaatgtgaa aaatccgctc tgttcagcca gtgataaata
     2401 ttgctatcgc gataacagct acaaacaggc gggatctatc gacggtagcc agatgttcca
     2461 cggtcctgcc tcactgtttg gcggcgtgga ataccagacg ccctggcaac cgctgcgcct
     2521 gaaactggag tatgaaggca ataattatca gcaggatttt gccggtaagc tggagcaaaa
     2581 aagtaagttt aacgtcgggg cgatttatcg cgttaccgat tgggccgacg ttaaccttag
     2641 ctatgaacgt gccaacacct ttatgtttgg cgtcacgctg cgcaccaact ttaacgatct
     2701 gcgcccgtct tacaacgata acgcccgccc gcaatatcaa ccgcagccgc aggatgccat
     2761 tttgcagcat tcggtggtgg cgaatcagtt aacgctgttg aaatacaatg ccggacttgc
     2821 cgatccgcag atccaggcga aaggcgatac gctgtatgtt accggcgagc aggtgaaata
     2881 tcgtgattcg cgcgaaggga tcgttcgtgc taatcggatc gtgatgaatg atctgccgga
     2941 tgggatcaag acgatccgcg ttacggaaaa tcgccttaac atgccgcagg tgacgacgga
     3001 aacggatgtc gccagcctga aaaatcatct cgccggagag ccgttgggcc acgaaacgaa
     3061 gctggcgcaa aaacgcgtcg agccagtggt tccgaagtcc accgagcagg gctggtatat
     3121 cgacaaatcg cgctttgatt tccatatcga tccggtgctg aaccagtcgg tcggtggccc
     3181 ggaaaacttt tacatgtatc agctgggcgt gatgggaacg gcagatttgt ggctgacgga
     3241 ccatctgctg accaccggca gcctgtttgc aaatcttgcc aacaactacg acaagtttaa
     3301 ctacaccaat ccgccgcagg actcgcactt accgcgcgtg cgtacccatg tgcgcgagta
     3361 tgtgcagaac gatgtctatg tgaataacct gcaagccaac tacttccagc atctgggcaa
     3421 cggcttctac ggtcaggtct acggaggtta tctcgaaacc atgtttggcg gtgcgggggc
     3481 agaagtgttg tatcgcccgc tggacagcaa ctgggcgttt ggtctggatg ccaactacgt
     3541 taaacagcgc gactggcgta gtgcaaagga tatgatgaaa ttcaccgact acagcgtgaa
     3601 aaccggacat ctgaccgcct actggacgcc atctttcgct caggatgtgt tagttaaagc
     3661 cagcgtcggg cagtatctgg caggggataa aggcggcacg ctggagatcg ctaaacgctt
     3721 tgacagcggc gtggtggtgg gcggctatgc cacgatcacc aacgtttcga aagaggagta
     3781 cggcgaaggg gacttcacca aaggcgtgta tgtctctgta ccgttggatc tcttctcgtc
     3841 tggcccgaca cgcagccgtg cggcgattgg ctggacgccg ctgacgcgtg acggtggtca
     3901 gcaacttggg cgtaagttcc agttgtatga catgaccagc gaccgtagcg tcaatttccg
     3961 ctaagtcatg ggaaaggtgc cagttttcgc aatcactggc acctttattc ttaatggcct
     4021 gttgagccat caggaacgca gttcaccccc ggtttgcgat ctggtgtgta aaggcgaaaa
     4081 cattctgtct tcctgtccgt cgatatcggt tgcaggtctg gttgcctgag cgactgggcc
     4141 atcattgggc tggagacatt gctttgcggt aggtcggctt tatcagcagt accgagcgga
     4201 ccagcataag caggaagagc agagactgat aacatcaaag cagcaaaata aggcttcatt
     4261 tttaccacct ttatcaggtt acgtttcatt tgttccagag gaacattgtc gatttttcgc
     4321 gcattgctgg tggctgggaa tcacctgaat gggtgatttt tgaattaccg gctttggtgc
     4381 ggtttgtctt gccggatgcg ccgccaggcg cggcttatcc ggcctacggg taggtatatc
     4441 cggctttggt ggaggcgcgc tccaaatcca ggttgaacaa aacatacaca aaaaatatag
     4501 atctccgtca catttttgcg ttatacagga agctcgccac tgtgaaggag gtactgctat
     4561 gacgtcactc tctcgtccgc gcgtggagtt tatctccacc attttgcaga ccgtactcaa
     4621 tcttggcctg ctgtgcctcg gccttatttt ggttgtcttc ctcggcaaag aaacggtgca
     4681 tctggctgat gtgctgttcg cgccagaaca agccagcaaa tatgagctgg tagaaggact
     4741 ggtggtttac tttctctatt tcgaatttat cgcgctgatt gtgaagtact ttcagtccgg
     4801 ttttcacttc ccgctacgtt actttgtcta tattgggatc accgccattg tgcgcttgat
     4861 catcgtcgat cataaatcgc cactggatgt gttgatctac tcggctgcga tcctgttact
     4921 ggtggtcacc ctgtggctgt gtaattcgaa acggctgaag cgggagtaaa aagacagcac
     4981 gccgaactgg cgcggcgtgc tggacaggaa gattacagcg tagcagtttg ttgtgttttc
     5041 ttcgtttccg gttcccagag ggcttccagc tcctcaaggg ttttaccttt ggtttccggg
     5101 acaaatttcc acataaacag tgctgccaga acgcccatac aaccgtaaat ccagtaggag
     5161 aaaccgttgt ggaaatgggc caccagccag gagtttttgt ccatcatcgg gaaggtccag
     5221 gagacgaagt agttcgccag ccactgggcc gccaccgcga ttgccagcgc tttacctcga
     5281 atagcattcg ggaagatttc cgacagcagt acccagcata ccggacccca ggacatggca
     5341 aaggcggcaa catagaacag catcgacagt agcgccacaa tacccggtgc ctgagtgtaa
     5401 aacgcggtac cgaggctaaa cataccgatt gccattccga gtgcgccgat aatttgcagt
     5461 ggcttacgac caaatttatc caccgtcata attgccagaa cggtgaaggt gaggttgata
     5521 actccgacaa taatggtctg caacagcgcg atatccgtgc tggcccccag cgttttgaac
     5581 acttccggcg cgtagtacag caccacattg atgccgacaa attgctggaa gatggagagc
     5641 ataacgccga tgacaatcac gcccacgcca aacatcagca gacgaccacc ggttttgcgg
     5701 ccatgatcca gggagtgttt aatttcctgt actgcctgag ttgcaagcgt gttgcccata
     5761 attttgcgca ggataccttc cgcctgttct tgcttgccgc gtgacatcag ccagcgagga
     5821 ctttctggca cggtatacag cagcattaag aacagcagtg cagggataca ttccgaggca
     5881 aacatataac gccagccgtc agtattcagc cagctggcat caccggaacg cgcaataaaa
     5941 tagtttacgc agtaaactaa aagctgcccg aaaataatcg caaactggtt aaatgaaacc
     6001 agtttcccgc gaatatgagc aggagcaagt tccgcaatat acattggtga aagcattgag
     6061 gctaaaccaa cgccaatacc gccaataata cgataaataa caaattccgg gacataacct
     6121 gccagataaa caggcactgt gttgtccggg tttatagagg taaaaccaag ttctggccag
     6181 gcagaaccta caccagaaat aaaaaacagg acagcagcaa tcttaagtga atcacgacga
     6241 ccgaagcggt tactgcaata accaccgagg gcaccgccga tgatgcaacc aatcagagcg
     6301 ctggccacgc aaaaccctaa cagggagttg gcagcggatt cacttaagtt ttgtggagca
     6361 acaaagacgg tattgagtga ctcaacagta ccggaaataa cggcggtgtc gtagccaaat
     6421 aataaaccac ctaatgtagc gactaaggta atcgaaaata tataactgga attatactgg
     6481 gtattcattc agacctgcct tagaccattc tgatctttta tcagtgcatt gatgttggaa
     6541 ttgtcaggca gttaaggcaa ttatcatttt ttacaatgcg tttacgtaat gtcttttctg
     6601 tgatcttaat tgtgataatt atccaaaatt gataaaaaca atactattgc cgtgactcag
     6661 agcacgaaag agaattatcg taagtgggaa aacaaataac gtaaaaataa taagctctat
     6721 gatggaaata ttaaccggcg aacgattcag attgcagacg aaagaaaaaa aggcgctccg
     6781 tggagcgccg aataacagtc acaagttggg ataacgtaag ttgagggtgc agcggcataa
     6841 cattggcaga acaacatctt taacctttca caccacctgc cgtcaggccg ttgaccagcc
     6901 agcgttgagc cagcaagaag acgatggtga tcggtaatgc agacatcacg gcagcggcgg
     6961 caaagtcacc ccacaggtag ttttgcggat tgaggtattg ctgcatcccc acggccaggg
     7021 tgtagctgtt tacgtcacgc agtaacagcg acgcgaccgg aacttcagta atggcagcga
     7081 taaacgacag gatgaatacc accgccagaa tcggtactga cagcggcaac aggacaaggc
     7141 ggaaggcctg ccacggtgtc gcaccatcca gcgcagccgc ttcttccagc gaactgtcga
     7201 tggtttcgaa atagcctttg atggtccaga cgtgcagcgc aatcccaccc agatacgcga
     7261 aaattacgcc gccgtgagta ttcaggccaa tgaatggaat gtactcaccc agacgatcaa
     7321 acaacgcata caacgcgacc agtgaaagta ctgccgggaa catctggaaa atcagcattc
     7381 ctttcagcag cgtcgctttg cctggaaagc gcatacgggc gaaagcgtag gcgcaggtgg
     7441 tggagagcgc cacaatgcca atcgcggaaa tcccggcgac ctttaccgag ttccacagcc
     7501 acagcagcac cgggaatggc ggtggcgtaa tgcgaccatc agcctgttcc acgctaaaac
     7561 ctaatgccag tttccagtga tcccaggaga tttgctccgg gatcaggctg ccggtcgcaa
     7621 agttcccctg gcgcagtgag atagcgacga ccatcagcag cgggaacata atcgccgcga
     7681 taaaaagtag cagtagcagg tgagtaataa acaaacgtgc tttttgcgat ttcggttgga
     7741 ccattgccat ttttgttatc tcccttaatc aaacttcatt cgcgtggctt tcaggttcac
     7801 tatcgccagc gcacccacca gcaggaagat cagcgtggca attgctgctg ccagaccgaa
     7861 gtcctgaccc ccgccgcctt caaaagcgat gcggtaggtg tagttaacaa gcaggtcggt
     7921 ataaccggct ggcgtggtcg tgccaagacg atccgggccg ccgttggtta acagttgaat
     7981 cagcacgaag ttgttaaagt taaaggcgaa gctggcgatc atcagcggcg tcagcggttt
     8041 aatcagcagc ggcagcgtaa tcttaaagaa gttctggaac ggacctgcgc catccattgc
     8101 tgaggcttca tacaaatcgt ccggaatcgc tttcagcaag cccatgcaga ggatcatcat
     8161 gtacggatag cccagccagg tattgacgat aatcagcatc gttcgggcgg tggtcggatc
     8221 gctgaaccag gcgggcttca cgccaaacag cgcgctcaac atcatgttga tctcaccgaa
     8281 gctctggtta aacaaccctt tgaaaatcaa gattgaaatg aacgatggca ccgcgtaggg
     8341 cagaatcagc aggacgcgat agaccgcttt gccgcgcaac gcttcccact gcaccagaca
     8401 cgccagaacc atgccgactg ccaccgttaa aaagacagtg atcagcgaga acaccacggt
     8461 ccagacgaaa atggcgagga acggtttctg aatgccttcg tcggtgaaga cgcgagtaaa
     8521 gtttttccag ccggtggtca cggtgtaacc ggggcttagc ttttcatcac cccagttgcc
     8581 gtcggcggta atggactggt aaaagccaat ttggttattc ggacgatatt tcacgccgct
     8641 ctgattattc gtcaacgtgc cgtcaccgtc gagtgtgtag agcggctgcg tgccagaaaa
     8701 ctggcgcagg gagctcatca tcactttgtt gccatccggc agaatggcgg taatgtcact
     8761 cagcgcctga cgattctggg taatcacgcg tagatttgcg cgttcgcctt cgggctgggc
     8821 ggtcgtttct ttcagttgca gtttttgctc tctgccaaat ttaaaagcgt cggagaggta
     8881 atttttgccg gtttcgccgt cgctgagcgc cagttgccac tcatcgcccg ccgggtaaag
     8941 accaaagtta taggttttgc ctgcttgcca ggagcgatct aacaacactt cctgtgcacg
     9001 ttcaaaagtc agctggttag tgctgctgta gttggtgaag gcaatggcga tggtgcagac
     9061 cagagggaag aggacgaata atcccattcc agccattccc gggtaaacat agcgccaggc
     9121 gtaggcttta cgattggcga aaatatacag ccccgctgaa ctcaatatca gcgtggtaat
     9181 ggcgaacagg tattcccctt gtgcgtacat taaaacaaca aggtaaccca ccagcaggcc
     9241 gagcagacct aacactgacc atttcagcgc gtcgctttgc caccaatgtt tctttttaat
     9301 gacatccatg gggttcttcc tcattccagg acggataagg ctttcatgcc ttatccgaca
     9361 acaactgcct gatgcgacgc tgacgcgtct tatcaggcct acatacgttt cggttttgta
     9421 ggccggacaa ggcgttcacg ccgcatccgg catttcacag cattacttgg tgatacgagt
     9481 ctgcgcgtct ttcagggctt catcgacagt ctgacgaccg ctggcggcgt tgatcaccgc
     9541 agtacgcacg gcataccaga aagcggacat ctgcgggatg ttcggcatga tttcaccttt
     9601 ctgggcgttt tccatggtgg ctgcaatacg tggatctttc gccaactctt cctcgtaaga
     9661 cttcagcgct acggcaccca gcggtttgtc tttattaacc gcttccagac cttcgtcagt
     9721 cagcagatag ttttcgagga actctttcgc cagctctttg ttcggactgg cggcgttaat
     9781 accggcgctc agcacgccaa cgaacggttt agatggttga cccttgaagg tcggcagtac
     9841 cgttacacca taattcactt tgctggtgtc gatgttggac catgcccacg ggccgttgat
     9901 agtcatcgct gtttcgcctt tattaaaggc agcttctgcg atggagtaat cggtgtctgc
     9961 attcatgtgt ttgtttttaa tcaggtcaac caggaaggtc agacccgctt tcgcgccagc
    10021 gttatccacg cccacgtctt taatgtcgta cttgccgttt tcatacttga acgcataacc
    10081 cccgtcagca gcaatcagcg gccaggtgaa gtacggttct tgcaggttga acatcagcgc
    10141 gctcttacct ttcgctttca gttctttatc cagcgccggg atctcttccc aggtttttgg
    10201 cgggttcggt agcagatctt tgttataaat cagcgataac gcttcaacag cgatcgggta
    10261 agcaatcagc ttgccgttgt aacgtacggc atcccaggta aacggataca gcttgtcctg
    10321 gaacgctttg tccggggtga tttcagccaa caggccagat tgagcgtagc caccaaagcg
    10381 gtcgtgcgcc cagaagataa tgtcagggcc atcgccagtt gccgcaacct gagggaattt
    10441 ctcttccagt ttatccggat gctcaacggt gactttaatt ccggtatctt tctcgaattt
    10501 cttaccgact tcagcgagac cgttatagcc tttatcgccg ttaatccaga ttaccagttt
    10561 accttcttcg attttggcga gagccgaggc ggaaaacatc atcgtcgtta atgcggataa
    10621 tgcgaggatg cgtgcacctg tttttatttt cataatctat ggtccttgtt ggtgaagtgc
    10681 tcgtgaaaac acctaaacgg actctagttt ctttatacgg caacctcttt ccatcctcct
    10741 tgcccctacg ccccaccgtt gctttgtgtg atctctgtta cagaattggc agtaatgtgg
    10801 agatgcgcac ataaaatcgc cacgattttt gcaagcaaca tcacgaaatt ccttacatga
    10861 cctcggttta gttcacagaa gccgcgttct catcctcccg cctcctcccc cataaaaaag
    10921 ctagggggtg gaggatttaa gccatctcct gatgacgcat agtcagccca tcatgaatgt
    10981 tgctgtcgat gacaggttgt tacaaaggga gaagggcatg gcgagcgtac agctgcaaaa
    11041 tgtaacgaaa gcctggggcg aggtcgtggt atcgaaagat atcaatctcg atatccatga
    11101 aggtgaattc gtggtgtttg tcggaccgtc tggctgcggt aaatcgactt tactgcgcat
    11161 gattgccggg cttgagacga tcaccagcgg cgacctgttc atcggtgaga aacggatgaa
    11221 tgacactccg ccagcagaac gtggcgttgg tatggtgttt cagtcctacg cgctctatcc
    11281 ccacctgtca gtagcagaaa acatgtcatt tggcctgaaa ctggcaggcg caaaaaaaga
    11341 ggtgattaac caacgcgtca accaggtggc ggaagtgcta caactggcgc atttgctgga
    11401 tcgcaaaccg aaagcgctct ccggtggtca gcgtcagcgt gtggcgattg gccgtacgct
    11461 ggtggccgag ccaagcgtat ttttgctcga tgaaccgctc tccaacctcg atgctgcact
    11521 gcgtgtgcaa atgcgtatcg aaatctcccg tctgcataaa cgcctgggcc gcacaatgat
    11581 ttacgtcacc cacgatcagg tcgaagcgat gacgctggcc gacaaaatcg tggtgctgga
    11641 cgccggtcgc gtggcgcagg ttgggaaacc gctggagctg taccactatc cggcagaccg
    11701 ttttgtcgcc ggatttatcg gttcgccaaa gatgaacttc ctgccggtaa aagtgaccgc
    11761 caccgcaatc gatcaagtgc aggtggagct gccgatgcca aatcgtcagc aagtctggct
    11821 gccagttgaa agccgtgatg tccaggttgg agccaatatg tcgctgggta ttcgcccgga
    11881 acatctactg ccgagtgata tcgctgacgt catccttgag ggtgaagttc aggtcgtcga
    11941 gcaactcggc aacgaaaccc aaatccatat ccagatccct tccattcgtc aaaacctggt
    12001 gtaccgccag aacgacgtgg tgttggtaga agaaggtgcc acattcgcta tcggcctgcc
    12061 gccagagcgt tgccatctgt tccgtgagga tggcactgca tgtcgtcgac tgcataagga
    12121 gccgggcgtt taagcacccc acaaaacaca caaagcctgt cacaggtgat gtgaaaaaag
    12181 aaaagcaatg actcaggaga tagaatgatg attactctgc gcaaacttcc tctggcggtt
    12241 gccgtcgcag cgggcgtaat gtctgctcag gcaatggctg ttgatttcca cggctatgca
    12301 cgttccggta ttggctggac aggtagcggc ggtgaacaac agtgtttcca gactaccggt
    12361 gctcaaagta aataccgtct tggcaacgaa tgtgaaactt atgctgaatt aaaattgggt
    12421 caggaagtgt ggaaagaggg cgataagagc ttctatttcg acactaacgt ggcctattcc
    12481 gtcgcgcaac agaatgactg ggaagctacc gacccggcct tccgtgaagc aaacgtgcag
    12541 ggtaaaaacc tgatcgaatg gctgccaggc tccaccatct gggcaggtaa gcgcttctac
    12601 caacgtcatg acgttcatat gatcgacttc tactactggg atatttctgg tcctggtgcc
    12661 ggtctggaaa acatcgatgt tggcttcggt aaactctctc tggcagcaac ccgctcctct
    12721 gaagcaggtg gttcttcctc tttcgccagc aacaatattt atgactatac caacgaaacc
    12781 gcgaacgacg ttttcgatgt gcgtttagcg cagatggaag tcaacccggg cggcacatta
    12841 gaactgggtg tcgactacgg tcgtgccaac ctgcgtgata actatcgtct ggttgatggc
    12901 gcatcgaaag acggctggtt attcactgct gaacatactc agagtgtcct gaagggcttt
    12961 aacaagtttg ttgttcagta cgctactgac tcgatgacct cgcagggtaa aggtctgtcg
    13021 cagggttctg gcgttgcatt tgataacgaa aaatttgcct acaatatcaa caacaacggt
    13081 cacatgctgc gtatcctcga ccacggtgcg atctccatgg gcgacaactg ggacatgatg
    13141 tacgtgggta tgtaccagga tatcaactgg gataacgaca acggcaccaa gtggtggacc
    13201 gtcggtattc gcccgatgta caagtggacg ccaatcatga gcaccgtgat ggaaatcggc
    13261 tacgacaacg tcgaatccca gcgcaccggc gacaagaaca atcagtacaa aattaccctc
    13321 gcacaacaat ggcaggctgg cgacagcatc tggtcacgcc cggctattcg tgtcttcgca
    13381 acctacgcca agtgggatga gaaatggggt tacgactaca acggcgatag caaggttaac
    13441 ccgaactacg gcaaagccgt tcctgctgat ttcaacggcg gcagcttcgg tcgtggcgac
    13501 agcgacgagt ggaccttcgg tgcccagatg gaaatctggt ggtaatagca aaacctgggc
    13561 cggataaggc gtttacgccg catccggcat aaaacaggtt gtcattatct gaaaggggcg
    13621 aaagcccctc tgattatcgg gtttagcgcg ctattgcctg gctaccgctg agctccagat
    13681 tttgaggtga aaacaatgaa aatgaataaa agtctcatcg ccctctgttt atcagcaggg
    13741 ttactggcaa gcgcgcctgg aatcagcctt gccgatgtta actacgtacc gcaaaacacc
    13801 agcgacgcgc cagccattcc atctgccgcg ctgcaacaac tcacctggac accggtcgat
    13861 caatctaaaa cccagaccac ccaactggcg accggcggcc aacaactgaa cgttcccggc
    13921 atcagtggtc cggttgctgc gtacagcgtc ccggcaaaca ttggcgaact gaccctgacg
    13981 ctgaccagcg aagtgaacaa acaaaccagc gtttttgcgc cgaacgtgct gattcttgat
    14041 cagaacatga ccccatcagc cttcttcccc agcagttatt tcacctacca ggaaccaggc
    14101 gtgatgagtg cagatcggct ggaaggcgtt atgcgcctga caccggcgtt ggggcagcaa
    14161 aaactttatg ttctggtctt taccacggaa aaagatctcc agcagacgac ccaactgctc
    14221 gacccggcta aagcctatgc caaaggcgtt ggtaactcga tcccggatat tcccgatccg
    14281 gttgctcgtc ataccaccga tggcttactg aaactgaaag tgaaaacgaa ctccagctcc
    14341 agcgtgttgg tcgggccttt atttggttct tccgctccag ctccggttac ggtaggcaac
    14401 acggcggcac cagctgtggc tgcacccgct ccggtgccgg tgaagaaaag cgagccgatg
    14461 ctcaacgaca cggaaagtta ttttaatacc gcgatcaaaa acgctgtcgc gaaaggtgat
    14521 gttgataagg cgttaaaact gcttgatgaa gctgaacgcc tgggatcgac atctgcccgt
    14581 tccaccttta tcagcagtgt aaaaggcaag gggtaattac gccccacagt gctgattttg
    14641 caacaactgg tgcgtctcct ggcgcacctt tttttatgct tcattccagg gatatgagcg
    14701 attttttata gtaactcact tcttcttcac taagaatatc cattatctca atgcattatc
    14761 agagattctt ttcctttcgc cggtagtgtc tggacgctca ggctactttt tccagttatt
    14821 tcatttctgt aatgcagagg ttttatgtta ggtcatatct caaagtttga cggcaataac
    14881 tctttgataa aacatggtgt ggtgcaagga aataatatag tagattttga tttactacgt
    14941 aattttaatg gggggccagg gttaaatcga gaaa
//
LOCUS       DAZFAB010000104        14751 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZFAB010000104 DAZFAB010000000
VERSION     DAZFAB010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14751
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.104"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            78..347
                     /locus_tag="ACHJWA_003752"
     CDS             78..347
                     /locus_tag="ACHJWA_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO4310268.1"
                     /translation="MSEQYLITLDEWKPKRFSLPITNTTLVKYGKLGYIVPRPQKIRG
                     RWLIDRRAVFVGPGETGIAPEIHTGDDDALKEILTHVTEATKKQH"
     gene            316..1434
                     /locus_tag="ACHJWA_003753"
     CDS             316..1434
                     /locus_tag="ACHJWA_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFO4310269.1"
                     /translation="MSPRPRKNSTDVAGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTD
                     KGKAEKIASTANQRIAAAEAEYFMRKIDESPSATKRRGIRLKAWVDRYLKIQDTRLKN
                     GDIAATTHKEKTRMAAYLVSRLGNHPLKELEVRDFALILDEWLDKDMVSTARVNRGLW
                     VDIYKEAQHAGEVPPGWNPPEATRKPIPKVTRARLTMEDWQKIYNATPEKHFIRNAML
                     LAIVTGQRRDDICHMRFSDVWNEHLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGC
                     RDRILSPYLIHSRHQKQPKPMSKDNLSDYFAKARDLAGVIPPAGKTPPTFHEQRSLSE
                     RLYRAQGIDTKTLLGHKVQATTDRYNDTRGQEWVKLVI"
     gene            1601..2395
                     /gene="potC"
                     /locus_tag="ACHJWA_003754"
     CDS             1601..2395
                     /gene="potC"
                     /locus_tag="ACHJWA_003754"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFO4310270.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            2392..3438
                     /gene="potD"
                     /locus_tag="ACHJWA_003755"
     CDS             2392..3438
                     /gene="potD"
                     /locus_tag="ACHJWA_003755"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFO4310271.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLSPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(3594..4415)
                     /gene="cobB"
                     /locus_tag="ACHJWA_003756"
     CDS             complement(3594..4415)
                     /gene="cobB"
                     /locus_tag="ACHJWA_003756"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFO4310272.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(4431..5342)
                     /gene="nagK"
                     /locus_tag="ACHJWA_003757"
     CDS             complement(4431..5342)
                     /gene="nagK"
                     /locus_tag="ACHJWA_003757"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFO4310273.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLPAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(5371..6615)
                     /gene="lolE"
                     /locus_tag="ACHJWA_003758"
     CDS             complement(5371..6615)
                     /gene="lolE"
                     /locus_tag="ACHJWA_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFO4310274.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(6615..7316)
                     /gene="lolD"
                     /locus_tag="ACHJWA_003759"
     CDS             complement(6615..7316)
                     /gene="lolD"
                     /locus_tag="ACHJWA_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFO4310275.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQLMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPWLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(7309..8508)
                     /gene="lolC"
                     /locus_tag="ACHJWA_003760"
     CDS             complement(7309..8508)
                     /gene="lolC"
                     /locus_tag="ACHJWA_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFO4310276.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            8770..9843
                     /gene="ycfT"
                     /locus_tag="ACHJWA_003761"
     CDS             8770..9843
                     /gene="ycfT"
                     /locus_tag="ACHJWA_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFO4310277.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLAALAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPVAVSYSR"
     gene            9971..13417
                     /gene="mfd"
                     /locus_tag="ACHJWA_003762"
     CDS             9971..13417
                     /gene="mfd"
                     /locus_tag="ACHJWA_003762"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFO4310278.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMEKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLENSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLESFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDTVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     LSRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            13561..14523
                     /gene="ldtC"
                     /locus_tag="ACHJWA_003763"
     CDS             13561..14523
                     /gene="ldtC"
                     /locus_tag="ACHJWA_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFO4310279.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTLPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(14606..>14751)
                     /gene="bhsA"
                     /locus_tag="ACHJWA_003764"
                     /gene_synonym="ycfR"
     CDS             complement(14606..>14751)
                     /gene="bhsA"
                     /locus_tag="ACHJWA_003764"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFO4310280.1"
                     /translation="ISANAGTNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAV
                     IYK"
BASE COUNT         3679 a         3842 c         3768 g         3462 t
ORIGIN      
        1 tcaggctgat ttttaatgtt caaccgtcgc cagttgtcgt tgatattctg caactggcgc
       61 gttccggagt gatagccatg agcgaacagt acctgataac gctcgacgag tggaaaccaa
      121 aacggttcag tctcccaata acaaacacta ccctggtgaa atacggaaaa ctaggataca
      181 tcgttccaag accacaaaaa attcgtgggc gttggctgat agatcgccga gcagtatttg
      241 ttgggcctgg tgaaacggga attgcgccgg aaattcatac tggcgatgat gatgcactga
      301 aggagatttt aactcatgtc accgaggcca cgaaaaaaca gcactgacgt agccggtctt
      361 tacgaaaagt ttgatcgcag aactggcaga gtttactacc agtataaaaa tcctgtgact
      421 ggaaaatttc acggactcgg aacagacaaa ggtaaggcag aaaaaatcgc ttccacagcc
      481 aatcagcgaa tagctgcagc agaagctgaa tatttcatgc gcaaaattga tgaaagtccg
      541 tcagcaacaa aacgtcgggg tatcagatta aaggcatggg ttgatcgata tctgaaaata
      601 caggacacgc gactgaaaaa tggagatatt gcagctacaa ctcacaaaga aaaaactcga
      661 atggctgcat acctggtttc ccgtctggga aaccacccat tgaaagaact ggaagtaaga
      721 gactttgcat taatactgga tgagtggctg gataaagaca tggtcagtac agcgagagta
      781 aatcgtggat tatgggttga tatttataaa gaagcacagc atgcagggga agttcctcct
      841 ggatggaatc ctccggaggc tacccgtaaa ccgatcccta aagtaaccag agccaggctc
      901 accatggaag actggcaaaa aatttacaat gcaacgcctg aaaaacactt tatccgtaac
      961 gcaatgcttc ttgcgattgt tactggtcag cgccgtgatg acatttgcca catgcgtttt
     1021 tcagatgtgt ggaacgaaca cttgcatatc acccagggaa aaaccggaat gcgtctggcg
     1081 ttaccgctta cactacgctg tgatgccatt gggataacgt taaaagaagt tattgatggg
     1141 tgccgagaca gaatattaag tccatatcta atccatagtc ggcaccagaa acaaccgaag
     1201 ccgatgagta aagacaacct gagcgactac tttgccaaag cacgggatct ggctggggta
     1261 attccaccag caggaaaaac tccgccaaca tttcatgaac aacgctctct atcagaacgg
     1321 ctgtaccgtg cacagggtat cgatacaaaa acattactag gacataaagt ccaggcaacc
     1381 accgatcgct ataacgatac tcgaggtcag gaatgggtta agttggttat ttgacaaaca
     1441 ccatatggaa tatccgtcac catctcagga aaaaagtgtt gtaaaatgcg tggcctagtt
     1501 ttggggaaac gttttggaga gattttggag aaaggaaaaa attgaataaa ttcaaaatcc
     1561 tgaaagtgtc ccgccttatg aataaaaagg tggaactcga atgatcggtc gactgcttcg
     1621 cggcggtttt atgaccgcta tctacgcgta cctgtatatc ccaatcatta ttttgattgt
     1681 gaactccttt aacagctcgc gctttggcat caactggcag ggttttacca ccaaatggta
     1741 tagcctgctg atgaacaacg acagcctgtt acaggcagcg cagcattcac taacaatggc
     1801 ggtgttttcg gcgacgtttg ctacgcttat cggttcactg acggcagttg cgctgtaccg
     1861 ttatcgcttt cgtggtaagc cgttcgttag cggaatgctg tttgtggtga tgatgtcacc
     1921 agatatcgtg atggcgattt cgctgctggt gctgtttatg ctgctgggta ttcagcttgg
     1981 cttctggtcg ctgctgttct cgcatatcac cttctgcctg ccatttgtgg tggtgacggt
     2041 gtattcgcgc ctgaaaggtt ttgacgtgcg gatgctggaa gcggcgaaag atctcggtgc
     2101 cagcgaattt accattctgc gaaaaatcat tctgccactg gcaatgccag cggtggcggc
     2161 aggctgggtg ttaagcttta ccctgtcgat ggacgatgtg gtggtttctt cgttcgtcac
     2221 cgggccgagt tatgaaattc tgccgttaaa aatttattcg atggtcaaag tcggcgtatc
     2281 gccggaagtt aacgcgctgg caaccatatt actggtgctg tcgctggtga tggttattgc
     2341 cagccagctt attgctcgtg ataaaacgaa aggtaacaca ggggacgtta aatgaaaaaa
     2401 tggtcacgcc acctgctcgc ggcgggtgct ctggcactgg gcatgagcgc cgctcacgcc
     2461 gatgacaaca acacgctgta tttctacaac tggaccgagt acgtgccgcc aggactgctt
     2521 gaacagttca ccaaagaaac cggtattaag gttatctatt cgacttacga gtcgaacgaa
     2581 accatgtatg ccaagctgaa aacgtacaaa gacggtgcct atgacctggt ggttccttca
     2641 acctattacg tcgataaaat gcgtaaagaa gggatgatcc agaagatcga caagtcgaag
     2701 ttaacgaact tcagcaatct cgatccagac atgctcaata agccgtttga cccgaataac
     2761 gattactcca ttccgtatat ctggggtgcg acggcgattg gcgttaacgg tgatgcggtg
     2821 gatccgaaat ctgtcaccag ctgggccgat ttgtggaagc ctgagtacaa aggcagcctg
     2881 ctgctgaccg acgatgcccg tgaagtgttc cagatggcgc tacgtaagct gggctactcc
     2941 ggtaacacca ccgatccgaa agagattgaa gctgcatata acgagctgaa aaaactgatg
     3001 ccaaacgtcg cagcgtttaa ctccgataac ccggctaacc cgtacatgga aggcgaagtt
     3061 aacctcggca tgatctggaa cggttctgct ttcgttgcac gccaggcggg tacgcctatt
     3121 gacgtggtgt ggccgaaaga aggcggcata ttctggatgg acagcctggc gatcccggca
     3181 aatgccaaaa acaaagaagg cgcgctgaaa ttgatcaact tcctgctgcg cccggatgtg
     3241 gcaaaacagg ttgctgaaac tatcggttat ccaacgccaa accttgcggc gcgtaagctg
     3301 ttaagtccag aagtggcgaa cgataagacg ctctccccgg atgctgaaac cattaaaaat
     3361 ggcgaatggc agaatgacgt tggcgcagcc agcagcattt atgaagagta ttatcagaag
     3421 ctgaaagcag gacgttaatt cagcaagcgg gcggtggtat taccgcccgt tttttagtta
     3481 gcaaggatgt ggctaatgag tcaggacagt aaagttttat cccgctcttt cgtcggtatt
     3541 gtggtccgct tgtaggcctg ataagcgtag cgcatcaggc aatgcttcct gctttataat
     3601 cccttcagca acttttcaac aaattctggc accacctggc ttgccgggcc gtaatatttc
     3661 tcggcaaatt cattaccgac ctgacttggt tcaagattca gctccacggt gtgcgcgcca
     3721 tgcagtttcg cttcgtgaac aaacccagcc gccggataaa catgcccgga agtaccaatg
     3781 gcaatgaaaa tatcggccat cgacaacgcc atataaattt catccatgcc gagtggcatt
     3841 tcgccaaacc atactacgtg tgggcgcaag ggggccggga actggcagca atggcattta
     3901 tcttctgggg taacgtctcc ggtccagtcg agaacctgac cactttgtga acaacgaact
     3961 ttcagcagtt ccccatgcat atgaatcaca ttggtattac ctgcgcgttc atgcaggttg
     4021 tctatattct gcgtcaccag caaaaagcga tcgccgagag catcttgcag tttagccagc
     4081 gcaagatgcg cggcgttagg ctgaatttct ggctgctgca gctgtcgacg acgggcgtta
     4141 taaaacgctt gcaccagttc aggatcgcga tcgaaacctt ccggcgttgc cacatcttca
     4201 acccgatgtt cttcccacag gccatctgcg gcgcgaaagg tacgaatacc tgattccgca
     4261 gaaattcctg cccctgtcag tacgagtact cttggttttt ccattgcttc cggcaccact
     4321 ttatctctga aaaaaatacg ctgacgcaaa cgctcgcgca ggcggcgttt atttttacga
     4381 aaacgactta accgatgacc ccgacgcgac agcatagcaa cctctgttgt ttaatcggtt
     4441 agatgtagga aggccgcacc acgcattcct cccgcatcac cgtggcgcgc gcgttcaatg
     4501 cgcggaacac gagctacagg taagagatga cgaggcagcc tgtccgccag ttgcgttgtg
     4561 attgccggga aattcgataa gccaccacca atgacgacca ggtcagggtc aacaatggtc
     4621 aggatatttc ccagacaaac cgctaataaa tccagataac gctcaacgtg cgcccttgcc
     4681 tgctcatcgc cttgatcata aagcgcaata atttcgggag ccggcaacgg ttgatgataa
     4741 tagtgttgat acagccacgc aaaaccgcga ccagacagat aattttcaat gcagccatgc
     4801 tgaccacagc cgcagcggcg taacgggaaa tccagcccca tcatggttaa cgcatcaacc
     4861 ggcagacgca tatggccaaa ctcgccggta atgtagcttt tcccggtaat cggtttgccg
     4921 ttgaaaatca gcccgccgcc aacgccggtg ccgagaatca accccatcac caacggatat
     4981 tgcgtaaatt cgtcatccca ggcttctgaa agggcaaaac agttggcatc gttatcaagg
     5041 cgtacatcgc gatcaagacg tgcgctcagg tcggcacgca gcggtttacc gctggcagca
     5101 gggacattgg cggcatacag cgtaccatct tctgtttccg gcattcccgg aataccgatg
     5161 ccgacagagc ctttacagcc aaaacgttga tcagcttcgg ctaccagctc gcacactgca
     5221 tctaaaaatg cgtcatagct gtcacgcggt gtcggcaccc gcttttccca ctgcaactgc
     5281 cgaccgctat caaacacgcc aagcgcaatt tttgttccac caatatcaaa cccgtaatac
     5341 attgccgctc cttgttttaa tgtactgcct ttactggccg ctaaggactc gcgcagggtc
     5401 aatattactg gcgcgccgcg ccggatacca acttgccaaa agactcagca acaatgctgt
     5461 gaccagtacg tagaagacgt ccagccagtg caattccgat ggcaggaagt caataaaata
     5521 gatatcgctg gagaggaact gatgaccgat cagcttttca atccgctcaa taatcggggt
     5581 aagttgcagt gaaacaacta cgccgataat cacaccacac aggctaccga atagccctgc
     5641 cagcaatcca taccagacaa agatggcgcg aattaaacca tctttcgccc ccagcgttct
     5701 taatactgcg atatcgccac tcttgtcttt caccgccatc actaaggtgg agacgatgtt
     5761 gaaacaggcc acgccaatca ccagtaccat cgccagatac ataatggcgc ggatcatctg
     5821 gatatcgcga tacatatagc cgtaagtacc aatccagctt ttaatataaa catagctgtt
     5881 ggtcacttca cccgcatcgc gtaccagctt attggcgttg aaaacatccg tcattttaag
     5941 ggcaatacct gacacgctgg aacccatatc aagatattgt tgggcatccg ccagcgggat
     6001 catggcaaaa ctgtgatcga gttgaccact caactgcaaa ataccggcaa cgtgcaaacg
     6061 cacacgtttt ggctgcatca gtttatgctc aggattcgag ttggggatca taatcgacac
     6121 ccaatcgccc tgcttcactt tcagcgcatc cgccacgcct ttgccgatga taatttgctg
     6181 ttcgcccgct ttaaaattgc gccaggcatc cccctgaaca aacgagggta atgcgctcag
     6241 acgctgttcc tgttgcgggt taacgccctt cacctggatt gcgcgcagat tcgctccact
     6301 ttccaccagc ccggtgaaat tgatatacgg cgcagcggcg gcaatacctg gcactttttg
     6361 cacgttatcc agtgcttcct gccagttagt ccacggttga tccaccgcct ctatttcgcc
     6421 atgcggcacc accgccagaa tgcggttatt cagttcgcgt tcaaagccgt tcatcgcgct
     6481 taagccgacg atcaataccg ccacgccaag ggcaatgcca atggtagaaa tgacggagat
     6541 cagcgacacc atgccgccgc gtcgccgtcc gcggctaaaa cgcaggccaa tcaataacga
     6601 taaaggcatc gccattactc cgcccccatc aggctcagtt ccgccgtcag acgaccatca
     6661 cgcatctcca gttggcggct catacgtttc gccagttgca ggtcgtgagt aaccaccagg
     6721 aaggcggtgc cctgcaagcg attcaattcc ccaagcaact ggaagatgct gtctgcgtta
     6781 cgcgcatcga ggttaccggt aggttcatcc gccagtacca gccacgggtt attgaccagc
     6841 gcacgggcaa tcgccacacg ctggcgttcg ccgccagaaa gttcagatgg gcggtgattc
     6901 gcacgatgct ccagccccac cgcttttaac atctcaagtg cacggctgtt gatttcagcg
     6961 ggctttttct tgccaatcag cagcggcata gccacgtttt ccagggcagt aaaatccggc
     7021 agcaggtggt gaaactgata aataaagccc agcttctggt tgcgcagttc tgctttcgcc
     7081 gccgaagaca gtttgctcat cagttgaccg ttaaagatca catcgccgga ggttggtgta
     7141 tccagcccgc ccagcaggtg cagcaaggta cttttaccgg aaccagagct accgacgatc
     7201 gccatcattt caccttcgcc cacgctgaaa ctgacattgt gcagtacatc ggtttgcaca
     7261 ctgccttcct gatagcgttt gcacaggttg tcgcattgca acaggatctt attcataacg
     7321 taaagcctca gcgggttgtg tggcggcagc gcgccatgaa gggtaaagcg tagacagcag
     7381 cgcgatagcc atcgccacca gcgcaataac aatgacctgt aaaggttcga tagccaccgg
     7441 cagcgccgcg ccatcaagca ggacgccgat tatcggcatc agattattta actggctggc
     7501 aagcagtgcg ccaagcgccg ctccgaggat cgcaccgata atcccggcgc tggccccttg
     7561 caccataaag accatcatga tttgtcgcgg agttaagcct tgcgtttgca ggatcgctac
     7621 ttcgccctgc ttctccatca ccatcagccc cagcgaggta ataatgttaa acgccgcaac
     7681 ggcgacaatc aggctcagca gtaagcccat catatttttt tccatgcgta cggcctggaa
     7741 cagctcgcct ttacgatcac gccagtcctg ccatttgctg ccttcaggca gtttttgctg
     7801 acttaaagag tcaactttca gcggctcatc cagccacaaa cgccagccgg taatattgcc
     7861 tgccggataa cgcatcagac gcgaggcatc ctcaatattc accagcattt catagccatc
     7921 gacttcactg ttagcggcaa aagtaccaat cacattgaac aggcgctgac ttggaatacg
     7981 ccccatcggc gtgaactggc tggcagatgg caccatcacg cggatttgat caccgcgatt
     8041 aacgcctagc tgtgaggcaa gttgttcgcc gaggatgaca ttatatttcc ccggctcgag
     8101 gtcagtttgt ttcacattga ccagatacgg cgttagtgga tctttttgcg ccggatcgat
     8161 acccagcatc accccgaccg ccacgctgcg cgcgctttgc agtaccacat caccggtagt
     8221 aataggtgcg acgcgattaa cgccgtccag tttgactgcc gtttccggga gttgctgcgg
     8281 gttaagagag ccatgctcag aagagagaat tgcctgtggc atcaggccaa ggatgttgtt
     8341 ttgcagctcg cgctcaaagc cgttcatcac tgacaatact gtgaccagcg ccatcacccc
     8401 gagggtaatg ccgatggtag aaagccagga gacgaaacga ccgaagcgat ccgctgcacg
     8461 cccacgcatg taacgcaggc caataaatag agcgacaggt tggtacatga aatccgtctg
     8521 gttgctgtag caaagacacg agtatataag cgaaagcaag atgggtaaac gtctgaactg
     8581 taagtgatcg tcagggctaa tctgaaaagc ccccgtaaat caatcggcta aattgccatg
     8641 ttttatcatc aatcttgccg cctgatctta atctgaaaaa tcatccaata cagacagata
     8701 cccattacac ctcgggtcat aacccgcagg atcaaccgca tgtaaattaa aaaagagcgt
     8761 gttgtcctga tgaaacaaaa agagctatgg attaaccaga tcaaagggtt atgtatttgt
     8821 ctggtggtga tttatcactc ggtcattacc ttttatccgc atatgaccac ttttcagcat
     8881 ccgttatcgg aagtcctgag caaatgctgg atctatttca atctttacct tgcacccttt
     8941 cgtatgccgg tttttttctt tatatccggc tatttgattc gccgctatat cgacagcgta
     9001 ccgtggggaa attgtctcga taaacggatc tggaacatct tctgggtgct ggcgctttgg
     9061 ggcgtggtgc agtggctggc gttaagtgcg ctgaaccagt ggctggcacc tgagcgcgat
     9121 ttaagcaatg cctccaatgc cgcgtatgcc gattccaccg gtgagttcct gcacgggatg
     9181 atcaccgcca gcaccagctt gtggtatctg tatgcgttaa ttgtctattt cgtggtatgt
     9241 aaaattttta gccgtctggc gctgccacta ttcgccttgt ttgtactgct gagtgtggcg
     9301 gttaatttcg ttcccacgcc gtggtgggga atgaacagtg tgatccgcaa tttgccttat
     9361 tacagccttg gtgcatggtt tggcgcaaca ataatgacct gtgttaaaga ggtgccgttg
     9421 cgccgccatc tgctgatggc ttctttgctg gctgctctgg cggtcggtgc ctggttgttt
     9481 actatctcgc tgctgttgtc gctggtgtcg attgtggtga tcatgaagtt gttttatcag
     9541 tatgaacaac gttttggcat gcgttccacc agtctgctga atgtgattgg ctccaacact
     9601 attgctatct acactaccca tagaattctg gtagagatat tcagcttaac tctgcttgcg
     9661 caaatgaacg cagcacgctg gtcgccgcaa gtcgaactga cactcctgct ggtttacccc
     9721 tttgttagtt tgttcatctg tactgttgcg ggcttgctgg taagaaaact ttcacagcga
     9781 gcattcagcg atctgttgtt ctccccgcct tcactgcccg ttgccgtcag ttactcccgc
     9841 taagccatcg ccccagccgg ggcgattcaa tttgctaaac catgtcgatt acggataatg
     9901 aacagcattg cttatcagag atatgccccc atatgttgag gcatatccta acgagaatct
     9961 gacaaccgtt atgcctgaac aatatcgtta tacgctgccc gtcaaagcgg gtgagcagcg
    10021 tctgctgggc gagttaaccg gcgctgcctg cgcgacgctg gtagcggaaa ttgccgaacg
    10081 tcacgccgga ccggtggtgc ttattgcgcc agatatgcaa aatgccctgc gtttgcatga
    10141 tgaaattagc cagtttaccg accagatggt gatgaatctg gcggactggg aaaccctccc
    10201 ttacgacagt ttttcgcctc atcaggacat tatctcctcg cgcctttcca ccctttacca
    10261 gctaccgaca atgcagcgtg gcgtactgat tgttccggtg aatacactta tgcagcgcgt
    10321 ttgcccgcac agttttctcc acggtcatgc gctggtgatg gaaaaaggtc agcgcctgtc
    10381 acgagatgca ttacgaaccc aactggatag cgccggttat cgccatgttg accaggtgat
    10441 ggagcacggc gaatacgcca cgcgcggcgc gttgctggat ctcttcccga tggggagtga
    10501 gttgccttat cgtcttgatt tctttgatga tgaaatcgac agtctgcggg tgtttgacgt
    10561 cgacagtcag cgcacgctgg aggaagtaga agcgatcaac ctgctgcctg cgcacgaatt
    10621 tccgaccgat aaagcggcga ttgaactgtt ccgcagccag tggcgcgata ccttcgaagt
    10681 gaagcgcgat ccggaacata tttaccagca agtgagtaaa ggcacattac ctgccgggat
    10741 cgagtactgg cagccattgt tcttcagcga accactgccg ccgctgttca gttatttccc
    10801 tgccaatacc ttgctggtga atactggcga tctggaaaac agtgccgaac gtttccaggc
    10861 tgacacgctg gcgcgttttg agaatcgcgg cgtcgatccg atgcgcccac tgttgccacc
    10921 acaatcgctc tggctgcggg tggacgagct cttctcagag ctgaaaaact ggccccgagt
    10981 gcagctaaaa actgaacatt taccgacaaa agccgcgaat gccaatttag gtttccagaa
    11041 actgccagac ctggccgttc aggcacaaca aaaagcgccg ctggatgcgc tgcgtaagtt
    11101 cctcgagtct ttcgacggtc cggtggtgtt ctcggtagaa agtgaaggcc gccgtgaagc
    11161 gctgggtgaa ctgctcgcgc gaattaaaat tgctccgcaa cgcattatgc gtcttgatga
    11221 agccagcgac cgtgggcgtt atctgatgat tggcgctgca gagcatggtt ttgtcgatac
    11281 ggtacgtaat ctggcgctga tttgcgaaag cgatctgctc ggtgaacgcg ttgcccgtcg
    11341 tcgtcaggat tctcgccgca ccatcaaccc cgatacactg atccgtaacc tcgcggaact
    11401 gcatattggt cagccggtgg tccatctgga gcacggcgtc gggcgctatg ccggaatgac
    11461 cacgctggaa gcaggcggca ttaccggcga gtatctgatg ctcacctatg ccaacgacgc
    11521 caaattgtat gttccggtgt cgtcgctgca tctgattagc cgttacgcgg gtggtgcgga
    11581 agaaaatgcc ccgctgcata aacttggcgg cgatgcgtgg tcacgcgcgc ggcagaaagc
    11641 ggcggaaaaa gtgcgtgatg tggcggcgga attgctggat atctacgcgc aacgcgccgc
    11701 caaagagggc ttcgcgttta aacacgatcg tgagcagtat cagttgttct gcgacagctt
    11761 cccgtttgaa accacgccgg atcaggcgca ggccattaat gcggtactta gcgacatgtg
    11821 tcagccgctg gcaatggatc gtctggtgtg cggcgatgtt ggctttggta aaacagaagt
    11881 ggcgatgcgc gccgctttcc tggcagtaga taaccacaag caggtggcgg tgctggtgcc
    11941 taccaccctt ctcgcgcagc agcattacga caacttccgc gaccgtttcg ccaactggcc
    12001 ggtacgtatc gaaatgctct cccgtttccg cagcgccaaa gagcagacgc aaatccttgc
    12061 ggaagtggcg gaagggaaaa tcgatattct gatcggtacg cacaaactgc tgcaaagtga
    12121 cgtcaagttt aaagatttag gcctgctgat tgtcgatgaa gaacaccgct tcggggtgcg
    12181 tcataaagag cgcattaaag cgatgcgcgc gaacgtggat attctgacgc ttactgcaac
    12241 gccgatccca cgtacgctga atatggcaat gagcggaatg cgtgacctat cgattatcgc
    12301 cacgccgccc gcccgtcgtc tggcagttaa aacctttgtc cgtgagtatg acagcctggt
    12361 ggtccgcgag gcgatcctgc gtgaaatttt gcgcggagga caggtttatt atctctacaa
    12421 tgatgtggaa aacattcaga aagccgccga acggctggca gaactggtgc cagaagcgcg
    12481 gatcgccatc ggtcacgggc agatgcgcga gcgcgaactg gaacgggtga tgaatgattt
    12541 ccatcatcaa cgtttcaacg tgctggtttg tacaaccatt atcgaaaccg ggatcgacat
    12601 cccgacagcc aacaccatta tcattgaacg cgcggatcac ttcggtctgg cgcaactgca
    12661 ccagttacgc ggtcgcgtcg gacgttcgca tcatcaggca tatgcatggc tgctgacgcc
    12721 gcatccaaaa gcgatgacta ccgatgcaca aaaacgtctt gaagcgattg cctcgctgga
    12781 agatctcggg gcaggttttg cgctggcaac gcacgatctg gagattcgcg gcgcgggtga
    12841 actgcttggc gaagaacaaa gcggctcaat ggaaaccatc ggtttctcgc tgtatatgga
    12901 gttgctggaa aacgccgtcg atgcactgaa agccggacgc gagccgtcgc tggaagatct
    12961 caccagccag caaacagaag tcgagctgcg gatgccgtcg ctattgccag atgatttcat
    13021 tcctgacgtg aatacgcgtc tgtcgttcta taaacgtatt gccagcgcca aaacggaaaa
    13081 cgaactggaa gagatcaaag tcgagcttat cgatcgcttc ggcctgctgc cggatccggc
    13141 gcgtaccctg ctggatattg cccgtctacg ccagcaagcg cagaaactgg ggatcaggaa
    13201 gctggaaggt aatgagaaag gcggcgtgat cgaatttgcc gagaagaatc acgttaatcc
    13261 ggcctggttg attggtttgc tgcaaaaaca gccgcagcat taccgccttg atggtccgac
    13321 gcgcctgaaa tttattcagg atttgagtga gcggaaaacg cgtatcgaat gggtacgcca
    13381 gtttatgcgt gaactggaag aaaacgcgat cgcttaatgg taaatcgggg gcgtttctgc
    13441 gcccccatct gcaacattta caaattcttt gcacttccct gcactatccg acactgtcac
    13501 catccataat tcaggcttat ctgtttatta caataacctt atatttatta tggatttttg
    13561 gtgatgatca aaacgcgttt ttctcgctgg ctaacgtttt ttacgttcgc cgctgccgtg
    13621 gcgctggcgc taccggcaaa agccaacacc tggccgctgc cgccagcggg cagtcgtctg
    13681 gttggcgaaa acaaatttca tgtggtggaa aatgacggtg gttctctgga agccatcgcc
    13741 aaaaaatata acgtcggctt tctcgctcta ttacaggcta accccggcgt tgatccttac
    13801 gtaccgcgcg cgggcagcgt gttaacgctc ccgttgcaaa ccctacttcc agacgcgccg
    13861 cgcgaaggca ttgtgatcaa cattgcggag cttcgtctct attactaccc gccgggtaaa
    13921 aattcggtaa ccgtgtatcc aataggtatt ggtcagttag gtggtgacac gctgacaccg
    13981 acaatggtga ccaccgtttc tgacaagcgt gcaaacccaa cctggacgcc aacggcaaac
    14041 attcgcgcgc ggtataaggc acaaggaatt gagttgcctg cggtagtgcc ggctggaccg
    14101 gataatccaa tgggccatca tgcgattcgt ctggcggcct atggcggcgt ttatttgctt
    14161 cacggtacga acgccgattt cggcattggc atgcgggtaa gttctggctg tattcgtctg
    14221 cgggatgacg atatcaaaac actctttagc caggtcaccc caggcaccaa agtgaatatc
    14281 atcaacactc cgataaaagt ctctgccgaa ccaaacggtg cgcgtctggt tgaagtacat
    14341 cagccgctgt cagagaagat tgatgacgat ccgcagctgc tgccaattac gctgaatagc
    14401 gcaatgcaat catttaaaga tgcagcacaa actgacgctg aagtgatgca acatgtgatg
    14461 gatgtccgtt ccgggatgcc ggtggatgtc cgccgtcatc aagtgagccc acaaacgctg
    14521 taaatcctgg ataaaaaaaa tccccgcgac acggcatgtc gcggggaaaa aatcagcagt
    14581 agcaggcatt aatgagggtt aatgcttatt tataaattac tgctgttcca tggagggtat
    14641 tcggaccggt tacagaagta atacggaaag attttgcgcc catctcatcc gctttttgcg
    14701 ccagctgctc ttccagcgat cccagatttg tccccgcgtt agcactgatt g
//
LOCUS       DAZFAB010000105        14605 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZFAB010000105 DAZFAB010000000
VERSION     DAZFAB010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.105"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            249..1079
                     /locus_tag="ACHJWA_003765"
     CDS             249..1079
                     /locus_tag="ACHJWA_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313311.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFO4310281.1"
                     /translation="MSMPLSNVLQSQIITDNHFLHHPKVESELTRKYERARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLDISKESWPFNYVTEACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIELGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1220..1993)
                     /gene="uxuR"
                     /locus_tag="ACHJWA_003766"
     CDS             complement(1220..1993)
                     /gene="uxuR"
                     /locus_tag="ACHJWA_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFO4310282.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2208..3668)
                     /locus_tag="ACHJWA_003767"
     CDS             complement(2208..3668)
                     /locus_tag="ACHJWA_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFO4310283.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3749..4933)
                     /gene="uxuA"
                     /locus_tag="ACHJWA_003768"
     CDS             complement(3749..4933)
                     /gene="uxuA"
                     /locus_tag="ACHJWA_003768"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFO4310284.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SH"
     gene            5273..6616
                     /gene="gntP"
                     /locus_tag="ACHJWA_003769"
     CDS             5273..6616
                     /gene="gntP"
                     /locus_tag="ACHJWA_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFO4310285.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(6790..7692)
                     /gene="fimH"
                     /locus_tag="ACHJWA_003770"
     CDS             complement(6790..7692)
                     /gene="fimH"
                     /locus_tag="ACHJWA_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFO4310286.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNQGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7712..8215)
                     /gene="fimG"
                     /locus_tag="ACHJWA_003771"
     CDS             complement(7712..8215)
                     /gene="fimG"
                     /locus_tag="ACHJWA_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HFO4310287.1"
                     /translation="MKWRKRGYLLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8228..8758)
                     /gene="fimF"
                     /locus_tag="ACHJWA_003772"
     CDS             complement(8228..8758)
                     /gene="fimF"
                     /locus_tag="ACHJWA_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFO4310288.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8768..11404)
                     /gene="fimD"
                     /locus_tag="ACHJWA_003773"
     CDS             complement(8768..11404)
                     /gene="fimD"
                     /locus_tag="ACHJWA_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFO4310289.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAVQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDRSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRSSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLARNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(11471..12196)
                     /gene="fimC"
                     /locus_tag="ACHJWA_003774"
     CDS             complement(11471..12196)
                     /gene="fimC"
                     /locus_tag="ACHJWA_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313302.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFO4310290.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     IIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGARVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(12233..12772)
                     /locus_tag="ACHJWA_003775"
     CDS             complement(12233..12772)
                     /locus_tag="ACHJWA_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4310291.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTPPGGNMQFQGVIIAETCL
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(12837..13385)
                     /gene="fimA"
                     /locus_tag="ACHJWA_003776"
     CDS             complement(12837..13385)
                     /gene="fimA"
                     /locus_tag="ACHJWA_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO4310292.1"
                     /translation="MKIKTLAIVVLSALSLSSTAALAAATTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLAQDGATSSAVGFNIQLNDCDTNVASKAAVAFLGTAID
                     AGHTNVLALQSSAAGSATNVGVQILDRTGAALTLDGATFSEQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
     gene            complement(13850..14446)
                     /gene="fimE"
                     /locus_tag="ACHJWA_003777"
     CDS             complement(13850..14446)
                     /gene="fimE"
                     /locus_tag="ACHJWA_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313299.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFO4310293.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTLERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
BASE COUNT         3636 a         3697 c         3454 g         3818 t
ORIGIN      
        1 atatagacct caatatagca acatcaaatt ccaattagta ataagatatg attttaaatt
       61 gcatgcaaat aattttaaat gagcgttttt tgatacttta gagtgtttcc taccctttaa
      121 tatcactgtg tagagttgat atcactacaa cagaaacatg catgtcattt gtttacttct
      181 ctttttacta cagaaaaatt tatctatttt tctaaacatc agtgaaatac tcataaaagg
      241 taactattat gtctatgcca ttaagcaatg tccttcagag tcaaattatt acagataatc
      301 acttccttca tcatccaaaa gtcgaaagtg agttaacacg taaatatgaa cgtgcacgcc
      361 tggatacgga aaatatctat cttctgcctc ttgctcgtgg caataaccat aattacgatg
      421 gaaaaagcgt tgtcgaaatt agaaaacttg atattagtaa agaatcctgg ccatttaatt
      481 atgtgacaga agcatgcaga gaatccgatg gtattacaac aacaggacgt atgctctatc
      541 gaaacttgaa aatcacctca gctttagacg aaatatatgg tggtatatgt aaaaaggcgc
      601 atgctgctac agagttagca gaaggtttac ggctaaattt atttatgaag agccctttcg
      661 atcctgttga ggactataca gttcatgaaa taacattagg ccctggttgc aatgttccag
      721 gttacgccgg aaccacgata ggatatattt caacccttcc agcttctcag gcaaaaagat
      781 ggaccaatga gcagccaaga attgatatct atattgatca gataataact gtctcagggg
      841 tagcaaattc ttccggattc gcccttgccg cattacttaa tgctaatatt gagttgggta
      901 atgacccaat aattggtata gaagcctatc caggtaccgc ggagatccat gcaaaaatgg
      961 gatataaagt tatccccggt gatgaagatg cgccactcaa acgcatgact ctgcaaccgt
     1021 catctcttcc agaactgttt gaattaaaaa atggtgaatg gaattatatt ggcaaataac
     1081 cactgagaag aatgagtact aacattcagg ttatatagat atttaaacaa taccctgcaa
     1141 tttacaaagt aatactcatt gcgacttctt aatttaagaa agcgcaagga acgtttaccc
     1201 ttgcgcttat tataataagt caggcgtcga ctttatccag cggccatgaa tcaaacagat
     1261 agccgtcaaa ataaatatcg tcaacgttcg agaattccag cagacgttgc ttaacgtttt
     1321 ccagatgctg ccacattgcc agcttcgccg ctcgggcatc ttttttgatt aacgcggcga
     1381 ggatctgttt gtgatcgccc aaccactctt tgcgatacag gctgtcatcc agatggctgt
     1441 gcaactggat ccacattggg ttgttttccc gccactgcca ggactgacgg aacagctcca
     1501 ccagcatgct gttatgcgtt gcttcggcaa tagcgagatg gaactgcatg tcaccgcttt
     1561 cgctgctgcc cggcgcactg gaagccagtt cacgctcttc cagttgcaat gcctgacgca
     1621 ttttgacgat atcttcgcgg gtagcctgca gggcggcaaa ctcggcgatg ttactttcca
     1681 gtaactgacg cgcctgtaac agctcaaaag gacccgcatc attgcagacg ttggcatccg
     1741 gactgtctgt gttgtggctg cctgagctat caagaacata gataccggca ccccggcgca
     1801 cttccaccag ccctttgatc tccagcatga tcagcgcttc acgtaccacc gtccgcgtga
     1861 catcaagcat ttctgcaatt tcacgctccg gcggtagccg ttcaccagga ttgtacggcg
     1921 tctttatgat cagatcgcgg atcatcgccc cgacttcctg gtaaggtctt tgcgcagagg
     1981 tggcagattt catggtgttg tgtccgttag gtgcggttaa tcaaattttt gccggatgcg
     2041 gcgtgaacgc cttatccggc ctacaacggg caattttacg gcccccgtta atctcaaact
     2101 ataccattag cactggaagt tggcacgtac aggtgggcaa aaaaacgaaa actggtcaac
     2161 caaatatcag tttgattgac cagtaagtct gtagggtaat cagttagtta cagcgcagcc
     2221 acacactcgc gcgcaccgcg ttcgcacagc tgctgatatg ccgcagtcac cgcgccaaca
     2281 aagtcggcat tctgcggcag atcatcggca aaaataccgc tcaggcccag cagcgctttc
     2341 acgcggtctg cgccctgata ctgcgcgtta atcttctgga actcggccag catcgggtcg
     2401 accacgtcaa tggcattacc ctgctcatcc acgccctggg tgtaacgcat ccagccagcc
     2461 acgcccagcg ccaggtgacg ccagctgccg ccgttttgca ggtgcagacg caccgggtcc
     2521 agcagacgct gcggtaactt ctggctgccg tccatcgcaa tctgccaggt acggtgacgc
     2581 agagacgggt tgctgaaacg ctcgatcagc agcgtcgcat aggcgttcag gtccgtacct
     2641 tccggcatcg acagcgttgg cgcttgttcc tgcatcatca gggcaaaggc cgctttgcga
     2701 taatccgggt tagtcatggt gtcggcaata gtttcatagc cgccgaggta accgaggtac
     2761 gccaggaaag agtggctgcc gttcagcata cgcagtttca tcatttcgaa cggcacaacg
     2821 tctgcaacga actgtgcgcc cactttatcc cagtccggac gaccattaac gaagttatct
     2881 tcaataaccc actgacggaa cggttcgcag gcaatggcgc acgggtcgta aacacccagc
     2941 tggtcagcaa tttcctgtaa ggtttctggc gtcgccgccg gaacgatgcg gtcaaccatg
     3001 gtgcacggga aggtgacgtt ctcttcaatc catgccgcta gctgcgggtc acgcgcctga
     3061 gccagcccca gtaccgcgac cttcgccaca tgaccgtttt cacgcacgtt atcgcaggac
     3121 atcaccgtaa atgctttcag ccctttttca cgacgcagac gcaaggcttc gacgatgtaa
     3181 ccaatcgcgg acttcggcgc agtcgggttt tccagatcgt gcttgatcag cgggttattg
     3241 agatccagtt gaccgcttgc cgcatcagcg cagtagcctt tttccgtgac cgtcagggag
     3301 acaatcgccg tttgcggacg cgccatcgcg ttgagaatac cttcgcagcc gtcgatttcc
     3361 gggtgcagcg cttctttcat cgaaccgata attttcagct cggtgctttc cgcgcctttt
     3421 tccgccacgg tgtacagcag ttgctgtttt ttcaggtttt cgatcagcac gcggtcgttg
     3481 cctggcatca ggttaacttc gcagataccc cagtcgctgt cggtgctttc cagcagatga
     3541 tgggtgtaca gcgcctggtg cgcacggtga aacgccccgc aaccgagatg cacaatgcgt
     3601 gattccagac gagaatgatc ccatgacggg cgggcaaccg gcagattgct gtcaacaata
     3661 gtagtcatcg taaactccag aatccgtctt cagagagtga cggcaaggaa gaaggagccg
     3721 gcacggcgcg cagccatgcc ggtgggtatt aatggctaaa gaaagcgcgc tggatcgcca
     3781 gttcgacacc gcgaacttcg gccaggcctt tcagacgacc aattgcggag taaccagggt
     3841 tggttttctt cttcaggtcg tccagcatct gatgaccgtg gtccggacgc atcgggatca
     3901 ggtcttcttt gccttccgct ttacgacggt gttcttcttc aacaatcgct ttcaccactt
     3961 cgtacatatc aacgtcaccg ttcaggtgcg ccgcttcgtg gaaggttttc gggttatctt
     4021 cacgcatggt ggagcgcaga tgggtgaagt aaatacgcgg accgaactgc ttgatcatat
     4081 caaccaaatc gttgtcagca cgcacgccgt aggaaccggt gcacatggtg aaaccgttcg
     4141 ccatgctgtt tacggtatca accatccact gcatatcttc aatggtggaa acaatgcgcg
     4201 gcaggccgag gatcgggcgc ggcggatcgt ccgggtgaac agccatacgg acgccaactt
     4261 cttcggcaac cggaataatc gctttcagga agacagcaaa gttttcgcgc agtttggctt
     4321 tgtcgatatc tttgtacagc tccaggtgct tacggaactg gtcgagggtg tacccttctt
     4381 ccgcacctgg cagaccagcg atgatgttac gggtcagacg cgctttgtct tcatcgctca
     4441 tagtggcgaa gcgttcagct gcctgagcaa tttcttcttc ggtgtaatcc gcttccgcgc
     4501 ctggacgttt caggatatgc atttcgaatg cagcgaattc gatctggtcg aagcgcagag
     4561 ctttggagcc gtctggcagc acgtattcga ggtcagtacg ggtccagtcg agcaccggca
     4621 tgaagttgta gcacacggtg cgaatgccgc actgcgccag gttgcgcaga gtctgctgat
     4681 agttagcgat ccactgctca tagttgccag tgtgggtttt gatatcttcg tgaattggta
     4741 cgctttctac gacagaccac accaggcctg cgtcttcaac gatcgccttg cgtttgagga
     4801 tctcttctac ggaccatact tcgccgttcg ggatatggtg cagcgcggta accacgccag
     4861 ttgcgcccgc ctgacggaca tcagctaaag aaaccggatc gtttgggccg taccagcgcc
     4921 aggtctgttc catgattttt cctctttcac atagtgggtt ccacagcaaa gagacgcgga
     4981 accctggagt tgtttaggta tccggatgaa caaccagacc gcagccgtgg cgttgcatcg
     5041 gatgagatgg cgtataagtt ctaccttttg gtaagacatg tcaacccgaa ttctgaaatt
     5101 ggttaaccac atcacaagaa tttcactctc atggttgttg ctgcaaattg cagcaaaggg
     5161 taatcgatgg ttattgatca gaggatgtga cattcatcgc aacaatggtt gaccaattta
     5221 cataacatgt cgcccaaaat aacaacggtt caaccaggta aggtaaaacg ttatgcatgt
     5281 gcttaacatt ctctgggtgg tattcggcat tggtctgatg ctggtactga atttgaagtt
     5341 caaaatcaat tcaatggtgg ctttgttggt ggcggcgctg tccgtcggga tgctggcggg
     5401 catggatttg atgtcgctgc tgcacaccat gaaagcgggc ttcggcaaca cgctggggga
     5461 actggctatc atcgtggtgt tcggtgcggt catcggtaaa ttgatggtcg actccggcgc
     5521 ggctcaccag atagcgcata cgctgctggc gcgtctcggt ctgcgctatg tacagctgtc
     5581 ggtgattatc atcggcctga tttttggtct ggcgatgttc tatgaagtgg cctttatcat
     5641 gttagcgccg ctggttattg tcattgccgc cgaagctaaa attccgttcc tgaaactggc
     5701 gatcccggca gtagcagctg ccactaccgc acattcactg ttcccaccgc agccgggtcc
     5761 ggtggcgctg gtgaatgctt atggcgcaga tatggggatg gtttatatct atggcgtact
     5821 ggtgacgatc ccaagtgtaa tctgcgcagg tctgatcctg ccgaagttcc tcggcaatct
     5881 tgagcgccca acgccatcat tcctgaaagc agatcaaccg gtagatatga acaatctgcc
     5941 ctctttcggc gtttcgattc tggtgccgct gatcccggcg atcattatga tctctaccac
     6001 catcgccaat atctggctgg taaaagatac ccctgcctgg gaagtggtta actttatcgg
     6061 ttcctcgccg attgcgatgt ttattgcgat ggtggttgca ttcgtactct ttggcaccgc
     6121 gcgtggtcat gacatgcagt gggtgatgaa cgcttttgaa agcgcggtga agagtattgc
     6181 aatggtgatt ctgatcatcg gtgcgggtgg cgtgctgaag caaactatca tcgacaccgg
     6241 cattggcgac accatcggca tgttgatgtc ccacggcaat atctcgccct acattatggc
     6301 atggctgatc actgtgctaa ttcgtctggc gacgggtcag ggtgtcgttt cggcgatgac
     6361 cgccgccggg attatcagtg ctgcaatcct tgatccagca actggtcagc tggttggcgt
     6421 gaatccggcg ctgctggtgc tggcgacggc tgcgggttcc aacaccctca cccacattaa
     6481 cgatgcatct ttctggttgt tcaaaggtta ctttgacctg tcggtaaaag acacgttgaa
     6541 aacctggggt ctgctggagc tggtcaactc cgtggttggg ctgattattg tgctgattat
     6601 tagcatggta gcgtaaaaga acaccagagc ctgccaatgg caggctcagt tcggattgtc
     6661 ggcaaagtgc gctttgttta tgccagatgc gacgctgacg cgtcttatct ggcctacaaa
     6721 gggctaacgt gcaggttttt agcttcaggt aatattgcgt acctgcatta gcaatgccct
     6781 gtgatttctt tattgataaa caaaagtcac gccaataatc gattgcacat tccctgcagt
     6841 cacctgcccg ccggtacgtg cgtaatttgc cgttaatccc agacttaccg ccgaagttcc
     6901 tactgctcct aacgataccg tgttattcgc tggaataatc gtaccgttgc gcgtcaactg
     6961 tacgccgacg ccctgcgctg gtgaaaacga cgcggtattg gtgaaaatcg agttgcccgc
     7021 atctgcggtt gtgccggaga ggtaataccc ctggttttgg cttttcgcac aataaacggt
     7081 aagaggaatt ggcactgaac cagggtagtc cggcagagta acggtgacat cacgagcaga
     7141 aacatcgcag ccgccagtag gcactaccac atcattattg gcgtaaatat tccacacaaa
     7201 ctggaaatca tcgctgttat agttgttggt ctgtcgcaaa ataagcacgg caattaatga
     7261 gccagcttta atcgccaccc cgcccgcact gctcacaggc gtcaaataaa gcgccaccgg
     7321 ccacggctta tccgttctcg aattataaac aacccgcggc gtttcgctgg tggtcggaaa
     7381 tggatagcta ctgccactat attttacggt cccggaaaaa ttagataaca cgccgccata
     7441 agccgagcct cgttgcagtg tgacatagtc tgtaatggtt tccggataat cgttatggca
     7501 aaagatttgc gtcgaaagat ctacgaccag gttttgcccc acattcacgg caggcgcaag
     7561 gtttacataa acattagcgc tgccaccgcc aatagggata gcggtaccat tggcggtttt
     7621 acaggcgaat gaccaggcat ttaccgacca gcccatcagc agtacagcaa acagggtaat
     7681 aactcgtttc attacaatta tctcttcggg ttcagctgta ggtataggtg atgctaatca
     7741 ctgcctgaat ggttccctga gtggctccgc catttaccgt caatgctctg acctgtaacg
     7801 ggaagtgcgc tgattgtgag gaatcatcca cctgaactgt tttggttgcg ccagtattca
     7861 atgtgttgcc actgtcatcc tgtagctcta actggatgtt ttgcgcggtc ccctggtttt
     7921 tataatatcc ggtactgtcg gctgccccgc tgaagctggc agtgaccctt gacgttccca
     7981 ccggacaatt agtcaactca agcgcaacat catgccaggc cgatgccgcc ccggcagaca
     8041 tcagactgaa agaataaaga tcgccgagat caaccgtggc attggtggtg gaaactgtgc
     8101 acggtttggc gacgacctta ccgttcaccg tgatggtgac atcggctgcc tgtatcgtcg
     8161 cacttgcgag cgccaatatt gccgccaata aatacccacg tttgcgccat ttcatgagca
     8221 tctccagtta ctgatattca agagtgaagg tagccgtggc attgatatgc cccgcagtga
     8281 caggcacctg tgtcgccatt agccgggcgt aaaaattcaa cgtatttggt ttacccggcg
     8341 tcagggtcgt ccacgaaatc gcggacgatg gagcattaag gggtatctga ttttgctgct
     8401 cattcagaag ctgtattccc agtcccgaag ccgctgacac cgtattttca agtgcaagca
     8461 ggttggcatt gtggctatct gcaacgccgg taaacccaac ttttacggca gaaacggcgt
     8521 taccacaggg tgacagcaaa atacgaaatg gaacgacagg agtcgtcgcg ccaatgttgt
     8581 taaattgctt cgccgcgttt tccatcagat caacagtaaa attggttgat tcagcggcca
     8641 cactacagcc gttatctctg acatagccgc ggatagtaat cgtgctatcc gcagccaaag
     8701 cgtgactcac tgccagccac aaaaaagcgc acagaagata aaaaggtttg tttctcatca
     8761 cgccccctta acgacattca gctgatagct gggttaataa ctgctgctga ctctctggtg
     8821 gcagttgata attggcgaca caatgagcat tttcctcttc tccccatttc acctgaactt
     8881 ttcccgctag aggcattccg ctgaggtaaa cctgaccatt atccgcaaca atgccgctac
     8941 tctggctact ctctgatgtc accatcgccc caaacggcag cggcttatta ttgtgggtta
     9001 gcgtcatgag cagttttatc ccaacgcgcg ctttaaactc tgctcgcacg atcgccccac
     9061 gagtgggaac aacgttagcg accgcgttat ctaaatcgac gttatcagcc agggtattgg
     9121 tatccagcgc cactctattt tcccgatatt cagtggcata aggcagcacg gcataaccgc
     9181 gccagtcggt acgcaccccc gtctggtttt cgacttttgc atcttttgcg ccaggcgctt
     9241 taacaagcac caccgtatcg tttaacggct gccccagcgt tacgccattg gcatgagcca
     9301 gtaccccacc gctgactccg taatagagct gcttaatatc atcgctatgg ctgtaaccga
     9361 tattggcatt gccgtaacca ccgcgataat tcagcgtggc gtagcctgtg cttccgctat
     9421 taccatcgcc tcccccggca tagccggttt gcacgctata gctgaggttg ttgtcttcca
     9481 gcaacgtacc gtatacacca gccagattgg tcatccgacc gttgagatcg tgtgacatgc
     9541 tgtagctggc actggcatgt cgccactgag atttactgtc agaacgcagc cagtggctga
     9601 aaggaatatt gacgttacgc gctaacatct gatcacgtcc tttttgccag gcgtttttcg
     9661 tcaggctata gctgagcgtc cagttgatat cttcgaacgc agtatttaat ccagcctgga
     9721 attgctcatc gacattactc gttccccaat aagtttgatg gctaccactc aaatacagtg
     9781 ttgatgagcg cccgagttgc tgagtaacgg tgagttgtaa tttcccgcgt ttgttataag
     9841 cgaggttgta atagtcggtg aatttcggct taacctgaat aactccgtcc tgtgtttcga
     9901 tgttgtagcc attcattcga ctgtatgttg tatcagcgaa attaaaatat ccgctggtcg
     9961 aataacggta acccactaac tgaatattcg tgcctgactc attgagcgat ttgttataga
    10021 gaaaacgcac cgattgtccg tcatgctgac tgtcatcggg aagtgtggaa ttagcctgag
    10081 tcatatccac agacagagcg cccagtgccc ccatattttt cccgatacca aaattaaaag
    10141 cacgataacg atctgccagt tgcgttccac catatattgt ccagccagct ggaaggccgt
    10201 ggagtaatgt actttggaaa aagcggggtt tttcctgttg cgcatttcca ctacggtatt
    10261 ctcctgccgt aatggaataa cgagtatgcc cttcacgttg caaaagcggg actgacgaat
    10321 agggtacggt aaaaatctgc gtgctgccgt cagcctcttt aatcgttacc tgcaagtcac
    10381 cactattacc tgcggcatag atatcgttga tggtaaaagg ccccggcggc accgtactat
    10441 tataaatgtc atacccattt tgtttaatag tgacctgtgc agtaccacga gcaataccgt
    10501 ggatcaccgg ggcaaatcct ctttggctat cgggtaacat attgtcatct gaggccaatt
    10561 gtgcgccgcg aaagttaata ccatcgaaaa tatcaccctg agtataacca tcacccagcg
    10621 tcagccggga acgtaacggt attatgtctc gctcaagcca ggtattgata tgctgccatt
    10681 tatttttgct acctgatgat ctgtcgctac tgttataact ccaggtggta ttgtcgcgta
    10741 aacgccacgc accaatattt aacccactct gtaggtttaa atatgcataa tggctgttac
    10801 ccccaatccg attctgtaca ctatttccgc tgaaattata attgagcaat ccggcattaa
    10861 taccgggatc ccataactca ggaggaatat aaccacgcgc gcgattactc ataaatgcct
    10921 gagggatcgt caggttcagt cgctgctgac caacatctaa atgcgcagta gcgtcctgga
    10981 ccattgtggt taatggcaca caggcatcat ccgccagcag attcataccg gcgacagaag
    11041 ccgtattcag ccccatactg gcgagttgcg cgcgtgtcag gcagggaaca atcccttgtt
    11101 cactgtcgcc cgtattaaat gtgacatcac gcgttgccat ataaccatta ttcaaataga
    11161 tatcgacgcg atacgtccct ggcggtaatt cttgcccatt ttcaaaacgc gataaatcgg
    11221 ccacagcctg gggatcatcc gctaaaaagc gcggattaaa atagagttcg gcagatgaca
    11281 aaggtgcctg tacggcaaaa gcacaggcga caaagagccg gacaaaaaaa ccagccaaac
    11341 gatgcttacg aatatgcaag cattgtgtgt ttcgctggta aagtcttaaa ttcagatatg
    11401 acattattcc tccggtttaa aatcacccgg cagtcaattc tttttattca ggcgaaaaat
    11461 tccccctgcg ttattccatt acgcccgtca ttttgggggt aagtgcacca taatcattta
    11521 ttgttcggta agtaatattg cttcccgcat cagaaggcaa tttaaccgtg ctttcgccca
    11581 ttggaggcac caaggcattt tcaagaaccc gggctccggc attcaactct gttaccgtca
    11641 ggtaataggg tgtcgggtta atcagcgtca gagaattcgc gctacgacga aatcttaatt
    11701 tttctgcggc ctgatcgggt ggcaacgcta atttagccgg acgatagtac agtttaatgc
    11761 ggctgataat tgcgagctgt agcgtattct cagtcaattt tgatttatcc attgacggaa
    11821 tcgctttaac gttcatccag aataaacttt cccggtcctg tggcaattgg ttatttgttg
    11881 catcaagaat acgtaaggta ttctcttttt ttcccttcat cgcaaacaga ggaggcgtca
    11941 cgataaaacg accatccttt acaccatcgg cattttccac ccatgattga attaaatagg
    12001 tactattttc atcattattt gtcacggcaa gttgcacttg tttttgccct gccggataaa
    12061 ttatgcgagt cgcacctaag gccactcccg cttcagcgcg tccggcaacc atcattgcca
    12121 tgaacatcag gatacctgcc agcaagcaga atgttatttc ctgcgatttc cttacattga
    12181 cgtttttatt actcactgtc ctgttcctgt tatcacatta tctgctgaac aattactgat
    12241 aggttaaaga gaaccaggcc tgtgcattgg cgatgccgcc agtaacccga cgcccggtag
    12301 cacgatattt ggcgatgaaa tgtagcgaag tagagcctga gtaaagccgt ttccagtttg
    12361 ctggaggacg attaatcggt acgaggtttc cttcatcatc aaacaacgct acgccaatat
    12421 tggtggctat ccctggcccc tctcccacgg aaagcacatc cggattttta ccatccgcga
    12481 caccgtgaaa cgccacaccc acacgttcac tcaccaccgt gctacattcc cgtaaatgaa
    12541 taacaaaagg caccggtgcg ctatcttccc ctaccgcatg aaaccggtta ctgctgattt
    12601 gccccatatt gaccgtcatt tgtttatcac cggcttcaat caggcaagtt tccgcaataa
    12661 tgacgccctg aaattgcata tttccgccgg gcggcgtggt attccattta tttccggcca
    12721 gagcaaacat tggcagcaac gacgctaata gaaataatct cttccttttc atcgttattt
    12781 ttatcgcaca agggtgggca tccctgcccg taatgacgtc cctgaacctg ggtaggttat
    12841 tgatactgaa ccttgaaggt cgcatccgca ttagcagcac ccggggttgc ctcgccgatt
    12901 gcataataac gcgcctggaa cggaatggtg ttagtaccgt tattcagggt tgtttgctca
    12961 ctgaatgtcg caccatccag cgtcagcgca gcacccgttc tgtccaggat ctgcacacca
    13021 acgtttgttg cgctacccgc agctgaactc tgcagagcca gaacgttggt atgacccgca
    13081 tcaatcgccg tacctaaaaa ggcaacagcg gctttagatg caacattggt atcgcaatca
    13141 ttcagctgaa tgttaaaacc gacagcagaa ctggttgctc cgtcctgtgc cagcgatgcg
    13201 gtacgaacct gtcctaactg aacggtttga tcaacagagc ctgcatcaac tgcgcaagcg
    13261 gcgttaacaa cttccccttt aaagtgaacg gtcccaccat taaccgtcgt ggcagcggcc
    13321 agagccgctg tagaactgag ggacagagcc gacagaacaa cgattgccag agttttaatt
    13381 ttcatgctgc tttcctttca aaaaactatt tctaaatcga catgggcagt cgttctgtac
    13441 actttgtttt gtcaacgagt ttgactgcca acactgcaca gttttccccc aaaagatgaa
    13501 acatttgggg caattttgac tcatagagga aagcatcgcg cacaaacttt ttcagtttat
    13561 ttgctggatt aatattctat tgttatcttt atttatagat ttttatattg catgaggtgg
    13621 tttttggaga gaagaatgag gaagatgcgt cgagccacag aaacgttagc tttacatata
    13681 gcggaggtga tgtgaaatta atttacaaga gaaataattt acatatcaaa cagttagttt
    13741 aaaattttta tgcttgagaa aaaatacgta acttatttat gatatggaca gtttggcccc
    13801 aattgtcttg tatttatttg ttttttaact ttattatcaa taagttaaat caaacttctt
    13861 ctctttttaa tttttcgttt atgagattat ttctttccca taatccggca aaacgagcag
    13921 cattactggc ggtataacgt acagtatggc gaatatttcg atgcccgaga taatcctgaa
    13981 ttaaacgagt atctgcacca cgctccgcca gttcataacc gcaagcatgc cttaacatat
    14041 gaggatgagt ctgcgttacg gttccagctt caataccggc atcacgaata atgcgatagg
    14101 cctgctggcg agaaagccga ctcccgcggc gagaaataaa tatggcgtcg gtccggtcag
    14161 cgcctttcca gttagcacgt tctagggtcc agcgttccac agcttcacgc tcatcaaaac
    14221 gtaacgggtg aacggtagaa aatccgttct tcagtcggcg aatatttatt cggccttcat
    14281 taaggtcaag atcctgataa tgcagatcaa gcagttcact aatacgcatc ccatgccgat
    14341 atgccaacag aataagacaa taatctctgg ctcccgttgc cccgtaacaa accgcctgca
    14401 tcatggcctg aacttcttta ccggtaagat aacgacgttt actcacaata gtagtactcc
    14461 taactgagat atatttgaat gaatacctat aggaaacctc aatcggtcaa aattagccca
    14521 atagataaaa agataaccac acacgaagtg atgtggctat cagtcaatta cattaaatat
    14581 catacaaaaa taaaatatca ctgat
//
LOCUS       DAZFAB010000106        14589 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZFAB010000106 DAZFAB010000000
VERSION     DAZFAB010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14589
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.106"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            99..455
                     /locus_tag="ACHJWA_003778"
     CDS             99..455
                     /locus_tag="ACHJWA_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO4310294.1"
                     /translation="MQWYLSVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLELPYLSMLYLLATFLPVLALAIRRLHDTDRSGAWALLFFVPFIGWLVLLVFFCTEG
                     TSGSNRYGNDPKFGSN"
     gene            complement(506..1402)
                     /gene="yhaJ"
                     /locus_tag="ACHJWA_003779"
     CDS             complement(506..1402)
                     /gene="yhaJ"
                     /locus_tag="ACHJWA_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFO4310295.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFNGK"
     gene            1507..2208
                     /locus_tag="ACHJWA_003780"
     CDS             1507..2208
                     /locus_tag="ACHJWA_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFO4310296.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQHLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRTYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            2231..2395
                     /gene="yhaL"
                     /locus_tag="ACHJWA_003781"
     CDS             2231..2395
                     /gene="yhaL"
                     /locus_tag="ACHJWA_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFO4310297.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2529..3839)
                     /gene="cyuA"
                     /locus_tag="ACHJWA_003782"
     CDS             complement(2529..3839)
                     /gene="cyuA"
                     /locus_tag="ACHJWA_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFO4310298.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHDG
                     VVFTQQACVAEGEQESPLSVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3867..5198)
                     /locus_tag="ACHJWA_003783"
     CDS             complement(3867..5198)
                     /locus_tag="ACHJWA_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO4310299.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFIIVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGLVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(5473..6837)
                     /gene="tdcG"
                     /locus_tag="ACHJWA_003784"
     CDS             complement(5473..6837)
                     /gene="tdcG"
                     /locus_tag="ACHJWA_003784"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO4310300.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKAQEELLSKTYYSVGGGFIVEEEHFGLS
                     HDVETPVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6909..7298)
                     /locus_tag="ACHJWA_003785"
     CDS             complement(6909..7298)
                     /locus_tag="ACHJWA_003785"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFO4310301.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(7312..9606)
                     /gene="tdcE"
                     /locus_tag="ACHJWA_003786"
     CDS             complement(7312..9606)
                     /gene="tdcE"
                     /locus_tag="ACHJWA_003786"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFO4310302.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDKNGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9640..10848)
                     /gene="tdcD"
                     /locus_tag="ACHJWA_003787"
     CDS             complement(9640..10848)
                     /gene="tdcD"
                     /locus_tag="ACHJWA_003787"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFO4310303.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSLRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10874..12205)
                     /gene="tdcC"
                     /locus_tag="ACHJWA_003788"
     CDS             complement(10874..12205)
                     /gene="tdcC"
                     /locus_tag="ACHJWA_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFO4310304.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLVLKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(12227..13216)
                     /gene="tdcB"
                     /locus_tag="ACHJWA_003789"
     CDS             complement(12227..13216)
                     /gene="tdcB"
                     /locus_tag="ACHJWA_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFO4310305.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(13315..14253)
                     /gene="tdcA"
                     /locus_tag="ACHJWA_003790"
     CDS             complement(13315..14253)
                     /gene="tdcA"
                     /locus_tag="ACHJWA_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFO4310306.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         3805 a         3883 c         3390 g         3511 t
ORIGIN      
        1 atcaaaacct ttataacatc cgaaaaagaa agatacgaat agcatttaca caatacccgg
       61 aatagacttt cgtcactttt aattaaaggg atgtttttat gcagtggtat ttatccgtat
      121 taaaaaatta tgttggtttc tctggtcgcg cacgtcgtaa agagtactgg atgtttactc
      181 tgattaacgc catcgtcggc gctattatca atgtcattca attgatttta ggtctggagc
      241 ttccatatct gtctatgctt tatttgctgg caaccttcct tcccgtcctt gcgcttgcta
      301 tccgtcgctt acacgatacc gaccgttcag gtgcctgggc cttgctcttt tttgttccgt
      361 ttattggctg gctcgtgctt ctggtttttt tctgcacaga aggtacttct ggcagcaatc
      421 gttacggaaa cgatccgaag tttggttcaa attaatctta gaattggggc gatatttcgc
      481 ccctttttat taacaataat aaatattatt ttccgttaaa aagtttggga atttcccgca
      541 gacaccagga tttcgcttcc cccatactgt cacgacgcca ggccataata atatcgatct
      601 cgctggtcga ttccgggctg acgacacgca accgcccttc cgcaatatct ttttcgacca
      661 tcggatacgg catcgtcgcc acgccaagcc ccgccagtaa tgcctgacgt ttatcttcaa
      721 tcgtgctcac cgttaagcgc ggctgtttgt ccagcagctg tacagtcaac accgggcgct
      781 cacgagcggt atccgccacc gcaattccac gatatttcac acgcgtcact tcagataacg
      841 gttccggctc ctgatgaatc gggtgatcag gcgcggcaac gtagacgttc attaacgtat
      901 agagcttgcg cgagttgatc tccgacgagg aacgaaaatg catatccggc gcgataacaa
      961 tatccgcccg cccctgctcc agccgttccc acgcccccgc cagcacttct gtgatgattg
     1021 ccagttgggt attggctttt gccgccagtt tgtcgattaa cgggaaaaag gcaggtgtcg
     1081 gtaccagcgc ttcggtcaca atggtgagat gcgtttccca accgcgcgcg agagcttccg
     1141 catcagtagt cagtttatct gcggcttcca gcaaaacgcg ccctcgctcc aacaacatcc
     1201 tcccgacatt ggtgaatttg gtgcgatggc ccgagcggtc aaacagcacc acatccagct
     1261 cttcttccag tttttgcatg gtgtagctaa gtgcggaagg cacgcgtccc agctcatccg
     1321 ctgccgccgc aaaactgccc cggcgatcga tcgcatccat aacccgtagt gcttccagcg
     1381 ttaatgccct ttctttggcc atttcgttct cattcaggaa atttgaacat accgggcaga
     1441 atatctggct aacaatgtag cgtccagccc cttaccataa aaggaagtaa agagaggtca
     1501 agaattatga ttactacccg aactgccagg cagtgtggac aagcagacta cggatggttg
     1561 caggcccggt atactttttc ctttggacac tacttcgacc cgaaattgtt aggctatgcc
     1621 tccctgcgtg tgcttaacca ggaagtgctg gccccaggtg ccgcctttca gccgcgaacc
     1681 tatcccaaag tcgatatttt aaatgtgatt ctggatggag aagcagagta tcgcgacagc
     1741 gaaggcaatc atgttcaggc cagcgccggt gaggcgttgc tgctctctac ccagccgggt
     1801 gtcagctaca gcgaacacaa tctcagcaaa gacaaaccgt taacacgaat gcagctttgg
     1861 ctggacgcct gcccgcagcg agagaatccg ctgattcaaa aactggcgct taatatgggc
     1921 aagcagcatt taatcgcctc gccagagggt gcgatgggaa gcctgcaatt acgccagcaa
     1981 gtgtggctgc accatatcgt gctcgacaaa ggcgaaagtg cgaatttcca gttgcatggg
     2041 ccacgcacgt atttgcaatc gattcacggg aaatttcatg cgcttacgca tcatgaagag
     2101 aaagcggcgc tgacctgcgg tgatggggcg tttattcgtg acgaggctaa cattacgctg
     2161 gttgccgatt ccccactgcg cgctttgctg atagatttgc ctgtctagtt gtttttacag
     2221 ggagatgatg atgagtaaaa aatcggccaa aaagcgccag ccagtgaagc ccgtggtggc
     2281 gaaagaacct gctcgcaccg ccaaaaattt tggctatgaa gagatgttga gcgagctgga
     2341 agccatcgtc gcggatgctg aaacgcgttt agccgaggat gaagctaccg cgtaaagaca
     2401 ggttgccgga tgcggcgaaa acgccttatc cggcctacaa aatccaacaa cattaacgaa
     2461 ttaaacaacc cgtaggccgg ataagatgcg ccagcatcgc atccggcacg atacccaaaa
     2521 cctggcgttt atctggcctt gctcgccata atctcgataa tctgccgatc cgtttgctgc
     2581 atcgaatggc ttgctaacgc acacaggttg gcaatcgact gctcaacatc atgcgccacg
     2641 atcccttcat tgccggtcac ggcggtatca tccagcgcca ttaacaccgc tttccacgca
     2701 gccgaagcac tggtcgaaac cttcatcgcg cagctgttcg acgcaccatc gcaaatcatg
     2761 ccgctgacat cgccgatcat actgctgatc gccatcgaga tggtttcata acgcccatcc
     2821 accagccatg ccatcccggc ggcggccccc attgctgcgg tcgttgcggc acacagcgca
     2881 gacaaacgcg gtaactggtt atggatgtaa attgcgctca aatgagaaag catcagcgca
     2941 cgcgccagcc gttcatcatc cgctccgaag tgttctgcta ccaccaccac gggcattgtt
     3001 gcggtgatcc cctggttacc cgagccggag ttactcattg ccggaagcgt ggcgccgccc
     3061 atacgcgcat cggatgccgc gctggtacga atcacaatgg atgaagagag atctttcgcc
     3121 agcaaaccgc gctcgcactg tttttccagc gtcgcgccaa tatgcagccc ccacttaccg
     3181 ctcaaacctt cctgcgataa cgcacaattt agcttcgcgg aatcgagaat aaagcggatc
     3241 gccgcaaacg ggacttcatt gacgaacttc aggatctcag ccagcgtcgt tctggaaagc
     3301 accgatagcg gagactcttg ctcgccctct gccacacacg cctgctgggt aaacaccaca
     3361 ccatcgtgcg tctcaatatg cacaatgttg gtatgcccgc cgacgatagt gacacacgcc
     3421 cacttctcac cgttccagac tttggcgcgt gagaagagga tttcattgca aggttcctgg
     3481 atcttaacgg agactttccc cgccgccagc agtgctttgg catcggcaat tgcctgcgca
     3541 gttgcgtctt tcagcacttc cagcccggcg ttagcatttc cacctaacgc ccccagcgcc
     3601 gccgcaatcg gcagccccac cattcccgtg ccgggaacgg tgacgcccag accgttcttc
     3661 atcagatttg gcgaaaccca ggcttctaca cgttcaaccg gaccttccag ttctgccgca
     3721 gcaaccgccg ccgccagcgc cagtgaaatc ggttcagtac atcccagcgc cggttttact
     3781 tcctcctgaa cggcgaggat gtaacgctgc cataacggat ttaaagtcga atcaaacata
     3841 ttaaaaacct taaaatttca ggtaaatcaa gaaaatgcca ggaacggaga aacacaaagc
     3901 aacaaaccag tgacgataat caggtacaga gacatccctt tgtatttgtg caatgccggt
     3961 actttgtaaa ccagccacgc cgggatcagg caccctacca agccgaaaat cgggctacag
     4021 atagaggtga agctcaacac cggtgcgttc agtacgatgg cgctccaggc cagcaaaatg
     4081 gcgaaaatca tgatgccgcg ctgaacaaga ttttcgttaa tcttctcggc aggcatcttg
     4141 cgacgcagga tgttcattac gatcccttgc gttgcttcgc gaaaacctaa atagacgcca
     4201 aagaatgcag tcatgacagc aaagatattg agaatgacgc tgaccacttt cacccacgct
     4261 gcgccgtcac cgctaataaa ctgtgcggca atcgccagcg cggaaatatt ctgctcgtag
     4321 gcttttaccg cttcgtcatg tcccatcgcc agcgtgaacg acacggcgta gaaaaagacg
     4381 atgataaaca aaatgccaaa cgcgatattc attgcccgca atgctttatg ccgcgctact
     4441 tcaatcgatt tttcccgcga gcgataagag atcaccatcg ggcttaacgt ctggataaac
     4501 agaatcgacg tcagggtaaa cggcagcgta ataatagcgt ttttcaccag cagccccagc
     4561 ggcggtagcg aaccgacgtt atacagatgc cacattccca ccatcgacac gcccagcgcc
     4621 gcgaccacca gcagcttggt cagcaccatg ccagtcgaaa ttttgaataa caatttctcg
     4681 ccgcgtgaag agatcgccac cagaatgcaa atcagtacca gaccataaaa gggactgtct
     4741 gacagcaacc cttccgtcac gccgaaggta tgcaggtagg aagcactatc gttggtgatg
     4801 gcggtggaat agacgaacat ccaaatcacc agcattacga aatagagtgc acctaacagg
     4861 atgccccagt ttttacctaa ataaccgcta atgacgctcg ggtaatcttt acattctggt
     4921 gattctgcca gcgtattaat aaacaaccgc tgaaacagat acattgccgg gtaaccaatc
     4981 accgatgaga gcaaaaatac ccacaatccc atcaaaccga cctgcaccgg gagaaaaaca
     5041 atccccgcgc caatcgccat cccgatactc ataatcaccc agccggtgtc agtactgtcg
     5101 aatttgatcg cttctcgcca ctcgctctca ctcattccag cacgacccgc cggggtcgaa
     5161 gcgtctgcaa tgacgccttt attcgatgca atttccataa tttctcgctc aatattttgt
     5221 agggcttatt atttttttcc gagccgcatc aaggcgatat gcggtctacg tttttgcagg
     5281 cgagttatta gaataaagaa atgatacgcg cgaggtcaga gaaaatcttc acaatcaaga
     5341 ctttgaaata acggagatgg ataagaattt tctactaaat taatcgcaag agaatattca
     5401 ctctctattt gcggcagatc acaaaaaaaa ggtgcacatt tgtgcaccca aggatgaaag
     5461 ctgacagcaa tgtcagccgc agaccacttt aatggccagt ccgccgcgtg atgtttcgcg
     5521 gtatttatcg ttcatatctt tgccggtttc atacatcgtc tcgatcactt tatcgagtga
     5581 aacacgcggt gccgaggtgc ggcgcatcgc catccgcgcg gcgtttactg ctttcacggc
     5641 attaatggca ttacgttcaa tgcacgggat ttgtacctgt ccggcaaccg gatcgcaggt
     5701 cagtccaagg ttatgctcca tcgcgatttc cgccgcattg catacctgcg ccggactgcc
     5761 gcccagtagt tcagttaacc ctgccgccgc cattgaacag gccacgccaa tctccccctg
     5821 acagccgacc tccgcgccag agatggaggc gttcatttta tacagcgcgc caatagcccc
     5881 ggctgcaaga aaatagcggg caattgaccg ctcgtttacc ggacgacgga acttatcgta
     5941 ataagccagc actgccggaa taatgccgca cgcaccgtta gtcggtgccg ttaccacgcg
     6001 cccgccagct gcgttttctt cactaaccgc cagcgcgtac atgttgatcc agtcgatgac
     6061 attcatcgga tcgttagaga tgttatcgct ggaaaccagc tgacgacgca gcgctacggc
     6121 acggcgcggc acattgagcg gaccaggcag cacgccttca gtgttcatcc cgcgttcaat
     6181 accgtcatgc atcacttgcc agatacgggc aaaaccggcg tcaatttccg ctttactgcg
     6241 cagcgctagc tcgttgtgca tcatcaggcc agaaatagac aggccgttgt aatcacacat
     6301 tttcagcagt tcacctgctg agtggaaatc gtaaggtacg ggtgtttcga catcgtgcga
     6361 caggccgaag tgttcttctt cgacaataaa cccgccgccg acagagtaat aggttttact
     6421 taatagctct tcctgtgcct tccaggcagt gatccgcatt ccgttctcat ggcgaggcaa
     6481 catttcggga tggaagataa tgttctttgc tacaggaaaa tcaacaatat gcgcaccaga
     6541 tgccactggc agccgcccgc tgcgcgttac taactctata aatgcaggga tctcatcaat
     6601 gacaacatcc tgcggactgt ttcctgccag tcccatgatg atggcgacat ccgtggcatg
     6661 gcctttgccc gtcagtgaca acgacccgta cagatcgacc acaatatggc tcgtcgcggt
     6721 taataagccg ctactttcca gccgatcaat aaaacttttt ccggcattca ttggccccac
     6781 ggtatgcgaa ctggagggac caatcccaat tttgaaaata tcgaatgcac taatcatatc
     6841 cacaccctcg gattgccgtt cagtgaagtg gagcggaacg accttacgac cgtcccgctc
     6901 acgaggcttt acgcactacg tactgcgata gcttcaattt ccagcttcac atctttcggc
     6961 aaacgcgcga cctgtacaca gctccgggtc gggtaggtcg cctgatgctc atcgaagaat
     7021 tgcttataga cttcgttgat ggtggcaaaa tcattcagat cggtgatgaa cacggtcatc
     7081 ttgatgatat cgcccacgct cagcccggca gcaaccacga tcgctttgac gttttcgagg
     7141 cttaaacgcg cctgatcttg cacatcagcc gggatctcac cggtctgtgg gcaaaccgga
     7201 atttgaccgg aggtgaagac catgctgcct aaatcaacgc cttgaacata agggccgatt
     7261 gcgcctgggg cacgttgcgt ttcgataatc tttttcatac atcctccggc gtcagagcgc
     7321 ctgggtaaag gtacgtgaaa taacatcctg ctgctgttca cgggtcagtg cgttgaagcg
     7381 cacggcgtag ccagagacac ggattgtcag gttaggatat ttttccgggt gctcgatggc
     7441 atccagcagc atttcccgat tcattacgtt gacgttgagg tgttgaccgc cttcgacatc
     7501 cgcttcgtgg tggaaatacc catccagcag gccgacaagg ttggttttac gtactggatc
     7561 ttctttgccc agcgccgcag ggacgattga gaaggtgtac gagatcccat ctttggcgta
     7621 ggtgaacggc agtttcgcca ccgacgtcaa tgaggccacg gcacctttgc ggtcacgacc
     7681 atgcatcggg ttagcgcccg gcgcgaacgg tgttccggcg cgacgaccgt ccggcgtgtt
     7741 accggttttc tggccgtaca ccacgttaga agtgatagtc agaatcgact gggtagggac
     7801 ggcgttgcga taggttggca gcgctttaat tttcttcata aaacgttcaa ccaggtcgca
     7861 ggcaatgctg tctacgcgct cgtcgttgtt gccgtactgc ggatattcac cgtcgatttc
     7921 aaagtccacc gccaggccgt ttttgtcacg gattggtttc acgcgggcat atttgatcgc
     7981 agacagggag tccgttgcca ccgacaggcc cgcgatgccg cacgccatgg tgcgatagac
     8041 atcacgatcg tgcagcgcca tcagcgaagc ttcgtagctg tacttgtcgt gcatgtagtg
     8101 aatgatattc agcgcgctga tgtactgcac cgccagccag tccatgaagt gatcgaggct
     8161 gtccatcact ttgtcgtaat ccagcacatc gtccatcaac ggtgccgttt tcggcccgac
     8221 ctgaatcttc agcttctcgt ccaccccgcc gttaattgcg tacagcagtg ttttcgccag
     8281 gttagcgcgt gcaccaaaga actgcatttg cttaccaatc accattgggc tgacgcagca
     8341 ggcaatcgcg taatcgtcgc tgttgaagtc agtacgcatc agatcgtcgt tttcatactg
     8401 caaggaagag gtgacgatcg acacctgcgc ggcatatttt ttgaaggcaa tcggtaattc
     8461 ttccgaccaa agaatggtca ggttaggttc cggtgcaggc cccatagtgt gcagggtgtg
     8521 caaatagcgg aaggagtttt tggtcaccag cgtacgaccg tccagcccca tcccgccgat
     8581 cacttccgtc gcccagattg ggtcgccgga gaacagcgaa tcaaattccg gcgtacgcaa
     8641 gaagcgcacc atacggatct tcatgatgaa gtgatcgatc agttcctgtg cctgctgctc
     8701 attgagtacg ccagctttaa agtcgcgctc aatgtagata tccaggaacg atgccgtgcg
     8761 gcccagcgac atcgcgccgc cgttctgtga tttcactgcc gccagataag cgaagtagag
     8821 ccactgcacc gcttcctgcg cattctgcgc cgggcgagag atatcaaagc catatttcgc
     8881 cgccatttcc tgaatctgca acagcgcatg acgatgctct gccagctcct cacgcagacg
     8941 gatggtggct tccagatcct cgcctttttc cagacgagac tggagatcgg caaattgcag
     9001 ttcgcgttca cgcaccagat aactgatgcc atacagcgct acgcggcgat agtcaccgat
     9061 aatgcgccca cggccatagc catctggtaa accggtcagc acgccagatt tacggcagcg
     9121 cagcatatcc ggtgagtaaa catcaaatac gccctggtta tgggttttac gcagatcggt
     9181 aaacagatat tcaaattcac tgtccatttc acgaccatag gcgtggaatg aacttttaat
     9241 catattaatg ccaccgaacg ggtgtaacgc acgtttcaac ggcgcatccg tttgcaggcc
     9301 aacaattttt tccagcggct ggttaatata tcccgcatca tgagcggtaa ttgtggtggc
     9361 aatattggta tcgaaatcaa ccggcgcgtg ggttgcgttt tcaatacgaa tgccttccat
     9421 caccttttcc cacaattccg tggtggctgg cgtcgcttca gcgaggaaag attcatcgcc
     9481 ttcatacggt gtatagttat gttgaataaa atcgcggaca ttaatttcgt ttttccagtc
     9541 cgtaccttta aagccaagcc atgcgtcggc gtacagctta tcgctggtat caatatctac
     9601 cttcatgaaa aataatctct ctacaatact tcaactaaat tatgcaaatt ctgcgggtgc
     9661 gttaactttg cctaaatgaa tggcatccaa agcaatcatt ttttcttcgt tggtcggaat
     9721 tacggcgcaa atgacacgcg cattttcact ggaaacaatt cgctcgccaa aagagttaga
     9781 tcgattattc atttctgtat caatctctac gcctaataca gccaaatgtt ccatgaccag
     9841 acgacgaatt aaacttgaat tctctcctat tccgccggtg aatataattc catccaggcg
     9901 acgtaatgaa gctgcgtgtc cggcaatatg acgggcaatt cggtgaacaa aggttttaat
     9961 tgccagctgc gcgcgttcgt gaccttcatg ccaggctttt tccagaacac gtaaatccga
    10021 agaaagaccg gaaataccta ataatcccga ctctttattc actacgcgtt ccaggtcacc
    10081 caggctctgg ttggtttggc tggcgaccca ggacatcgca ccaaagtcga catcgccact
    10141 gcgggtaccc atcatcaagc cttccagcgg cgtcattccc attgaggtgt caacactctg
    10201 accgttgcga actgcgcaga ttgacgcgcc attgccaaga tgcgccacaa ccaggccgga
    10261 gtcatcttcc gccagattca gcaaagaatg tgcgcgcagg gaaacatagc ggtgcgacgt
    10321 gccgtggaaa ccataacggc gtacacctaa ctcttcataa tatttccacg gcaggccgta
    10381 taaataagct tccggagcca tcgtctggtg gaaactggta tcaaataccg ccacctgagt
    10441 tacgcccgga aataattgct gcgccgattc aataccactt aaattggcgt aattatgcag
    10501 gggtgccagt ggagaaacgc gacggatatt atcaatgact tcatcggtaa taatggcgga
    10561 ctcggtaaaa atactgccgc cgtgagcgat gcggtggcca attaaggcca cgctgtcatt
    10621 taaattccgt ttttccagtt caaatgcaat tgccttcaat gcaccttcgt agctgtggtg
    10681 agccagcggt gctggctctc ccccatttac ggataagaat gcattttccg agttaatacc
    10741 gtcggcaata cctgacatta atacttcaca gtcgctggca tcgagtacgg aaaacttaat
    10801 cgaagacgaa ccacagttaa taaccaaaac aaccggaaat tcattcatct cttttctcat
    10861 cctgagttac ggattaaaac agtttatata cgatgttcag gatggtcagc agaccaatca
    10921 cggtaacaaa cacgttatcc agacgaccac ggtatttcgc cagagacggc gctttacgga
    10981 tggcatacat cggcaacagg cacagcaggg atgcgataat cggtgcgccc atggcttcaa
    11041 tcaggtcgag gatgttcggg ttggcgtagg caacaaccca ggtggagccc atgatgaaga
    11101 tcatgctgat agtgttcagt ttacccagcg atactttagt tttgtcgcct ttataaccaa
    11161 acttcaggac caggccattc agaccttcca gcgttcccag atagtgaccg aagaaagatt
    11221 tgaagatagc cacgagtgcg atgatggaag ccgcatattc cagtgtaatc gcgaacgttg
    11281 ttttggtacc ggtcatggac gcaaagtggt tagccagata agaaagcact ggaatattct
    11341 gcgctttggc ttccgccatg ttggccggag acagcgtaaa caggcagcta aaggcaaaga
    11401 acatcaccac tgcaaccatc agcatgctgg cacgagaaat gatttgggaa catttacgtt
    11461 cggtgaagtc gcgaccgaag tctttctcat actcttcacg tttagaaacc acgaaggaag
    11521 agacgattgg cgagaagtta aaggagaaaa ccatgatgga aatccccagc cagacagtga
    11581 tcaggatacc gtcatgaccg gttaacgaca gcgaaccgag gtcaacctgg tcgataactg
    11641 cagagttcca gtaagggatc agcgacaaag aaatcagcac caggctggcg ataaacggcc
    11701 ataccaggta gctcatcact ttaaccatca gatccttacc aaaccagatg acgaaagcca
    11761 tcagcagcaa caggaacagc gccacaaagc cgcgattcag cggtgcaaag ccgagctggt
    11821 tttcccagaa cgtcataaag gtattggtaa tagtaacgcc ataaatccac agcagtgggc
    11881 aaatcgcgaa gaagtacagg aacgtgataa ccacgccgcc agttttacca aaatgctctt
    11941 ccaccgtttc cgtaatgttg ccggaagggt tagagccaga aagacacaga cgcgccagcg
    12001 cccggtggca ataaaatgcg atggggtatg ccaataccaa catcagaaga atcgggatca
    12061 gtccgccaaa acctgcgcgg atagggaaga acagcacccc ggcgccgatt gccgtaccaa
    12121 acaagcctaa cgtccatgtg gtatctgatt tacgccagga cgattgtttt gtctggctgg
    12181 atacaatgct atctgaagta ctcatatcct atcctcaacg aattaattaa gcgtcaacga
    12241 aaccggtgat ttgagagacg cgagaaagat cgatattgcc gccggaaata atactgacgg
    12301 tttttctgtt ttgaatatat tggtctaatt taccgcttaa taatgcagca catgccagag
    12361 cgcctgcgcc ttcggtgacg actttattgc gctgaattaa ggcaatcata ctgtttctga
    12421 tttcgtcttc gctgaccagc acgatgtcat cgactaattc acgaacgatt tcgtaagtta
    12481 aattacccgg gcgggagaca tcacaaccat ccgccagggt gccggtagtt cggtgcgtgg
    12541 ttatttctcc ggagtggaaa gaagccgcca tgccgtgaac gttttcagac tgtacgccaa
    12601 taacacgaat ggtcgggtta atagatttaa ttgccaccgc aataccagca attaaaccgc
    12661 caccaccaat tggcacaatc acgttatcga catcatagag atcttccata atttccaggc
    12721 caatcgttcc ctgaccagca atcactttcg gatcatcgta aggtgggata aaaatacggc
    12781 cttccatttc gacaatttcg ctcactttag cgatagtgtc gttgaagtta tcaccatgca
    12841 gaacgacttc tgcggagtag tcgcacgttg ccgctacttt ggatttcggc gcgccttttg
    12901 gcatcaccac tttaccgtcg atacccagca tcgcgcagga gagcgaaacc ccttgcgcat
    12961 ggttgcccgc agaacaggcc accacgcctt tgcgtttttc cgcatcagtc agtgaactta
    13021 atttattaaa tgcgccacga attttaaatg aacccgtacg ctgcatattt tcaaacttca
    13081 ggaatatttc acctttgcaa cgttcactaa aatagttgga gcgaggcatg cctgttttat
    13141 aaattcgccc agccagtcgt tgtttcgctt caataatgtc atcaatagca accggcagat
    13201 cgtatgtaat atgcattata aaacctcttc gcctgaatta aatatgtagg taaccgtaac
    13261 cgacacctgc aagacagaca ggtggattat ttatgtttaa ataaaaacaa ataactaacc
    13321 aacttctatt aattgccttc gtctacaccc attataagat gaatactctt tggctaattc
    13381 caccaaaacc gatgctgctt ttttaatacg ataatttttc gaccataccg cggcatattg
    13441 tgccacaggt aatgtttctt caaccggaat agtaataaat tgattagaac caaaaggaga
    13501 cgtcatatca caaggaatta cagttaagaa atcagcattg agaacaagat tataaattgt
    13561 cacgactgag tcggttttaa cgatgttttc aatactgatg ccatttcttt gtaacgtagt
    13621 aagcagttcg ctgtagtacc ccatattagt ttgtggcaac acccactgtt cgttcttcaa
    13681 cgactccagc gtggtggtgc cggtgcatgt tcgggactta ctggctacca gcacaaactc
    13741 ggactcgaac agcggctcaa catgtaaatc ctgaagcttc atttctgcgc ttaacgtacc
    13801 aatcgcaaaa tcaagtcgac cgtcgcggat tgccggtaag aacgaagaca gttgcgcttc
    13861 atacatagaa acctgcgctt tcgggaacac ctctttgaac ttgttgatca tccctgacat
    13921 aaaagtaaaa ccaatcaatg aaggaaaacc aaatgagact tccaccaccg cctcagaaga
    13981 cataccgctt atctcattaa ccatattttt catttcacgg gtaatggatt cggaacgaga
    14041 gagtaacaat tgaccggcag gtgttaatgt tactccggta tttttccgca ccactaattc
    14101 cacaccaaaa taatcttcaa tatcgttaat gattttactg acggccggtt gagttaaccc
    14161 taattctttt gcagccgagc cgatagaacc acttctaatg acttcctgaa agactaccag
    14221 gtgctgcgtt ttcggaagaa gaatagtgct cataacgacc tacgttaatt acctcattga
    14281 cggcatgaag tgtatcaaaa tgaaatgaac aggatatgtg cgaccactca caaattaact
    14341 ttcaatactt tccagggtat cgtcatgtca attaaatacc tttaaatatc aataaattaa
    14401 agtataaaaa tcagcataaa ccctgatttt tgtcaaaaaa aatgacgggg ataaccatat
    14461 tttatggcga taacatcatt cgttatgtgg ttaatacaaa aaaggggctg agaccatatt
    14521 tcaagcaatt accggataat taccaggcaa aatttgagtt aaatttaatg agcaagttct
    14581 caaatttta
//
LOCUS       DAZFAB010000107        14566 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZFAB010000107 DAZFAB010000000
VERSION     DAZFAB010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14566
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.107"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..1794
                     /gene="espX6"
                     /locus_tag="ACHJWA_003791"
     CDS             <1..1794
                     /gene="espX6"
                     /locus_tag="ACHJWA_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector pentapeptide repeat protein EspX6"
                     /protein_id="HFO4310307.1"
                     /translation="SHHCNVKTEQLQQFIKIMEQKAQLPIYIDKNNLKESIFSVLHND
                     PQQVDKDQHLPCEKFLKHACKSSNSFEVKLDATHQYQHLNNFMISFDPVENQLTIRDN
                     NNKTETFSFTNLQWENLLQYYKENHQQPNIAGSRNLTDNIDKIKNTISTSEIIECASP
                     EIRSSVLNDLYSIANFLPDNNLTPNESWKRFCETCERFYVAQKSITGDKSERLTRKLS
                     ISDAGITMTFKIGDVVINTISTAIPEDATGQRCIEGLNLAEMDLTDIDLSKMALRNVN
                     FNGSILRNAKFSGTICEGVDFTDCDLRNAEFENASLENNDFRKVRHLTYVNFKNANLR
                     NSNFNGKVLTGVTFTGSDLSNAYLEHIDFTTVILYETSKIPGIPGTPQIPGTPKVILT
                     GAILNYSDLSGKDLSEYNLTGILCMYTNFSNANLTNCKISNANFSNAKFYNTNCTGAN
                     CSNILFDYAWFDNTIFIKTLFKNTCFYNVRAKNVYLEGAYLNNDNIVNQANNSTEKQS
                     IDSTDKQANDSTVQQSIDSTVQQANDSTDKQANDNIDKQVNDSTDKQAKNSTEQQDSN
                     SFNQARLKKEVNRRFSIPGLTSYQPTYIVEE"
     gene            2060..2902
                     /locus_tag="ACHJWA_003792"
     CDS             2060..2902
                     /locus_tag="ACHJWA_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313321.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFO4310308.1"
                     /translation="MKKRITFIVFLCAIVAASLFFVQSCVRKSQHVAGFQNYQATIDG
                     KEITGVTKNISSLTWSAQSNTLFSTINKPATIVEMTTEGDLIRTIPLDFVKDLETIEY
                     IGDNKFVISDERDYAIYVISLNADSEVSILKKIKIPLQETPTNCGFEGLAYSSQDHTF
                     WFFKEKNPIEVYKVTGLLRSDELHISKDKTLQRQFTLDDVSGAEFNPQKNTLLVLSHE
                     SRALQEVTVRGDVIGEMSLTKGKYGLSHNIKQAEGIAMDDSGNIYIVGEPNLFYRFTS
                     TKSR"
     gene            2970..4148
                     /gene="yjiJ"
                     /locus_tag="ACHJWA_003793"
     CDS             2970..4148
                     /gene="yjiJ"
                     /locus_tag="ACHJWA_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4310309.1"
                     /translation="MNSTSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMLAE
                     GEFSFSELSWIASGNYAGYLAGSLLFSFGAFHLPSRLRPFLLASALATGLLILAMAWL
                     PPFLLVFIIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGVGIALGNEYVLA
                     GLHFALSSQTLWQGAGALSAIILLALALLIPSNKHVIPPAPLAKIAQQPMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGQPVLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAICVLLTLASSSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALASATLCGAAALFIAALICGMQIFKLHTNYS
                     "
     gene            4359..9600
                     /gene="iatD"
                     /locus_tag="ACHJWA_003794"
                     /pseudo
     CDS             4359..9600
                     /gene="iatD"
                     /locus_tag="ACHJWA_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168882.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter IatD"
     gene            complement(9757..10352)
                     /gene="kptA"
                     /locus_tag="ACHJWA_003795"
                     /pseudo
     CDS             complement(9757..10352)
                     /gene="kptA"
                     /locus_tag="ACHJWA_003795"
                     /inference="COORDINATES: protein motif:HMM:NF014001.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
     gene            10557..11240
                     /gene="yjiH"
                     /locus_tag="ACHJWA_003796"
     CDS             10557..11240
                     /gene="yjiH"
                     /locus_tag="ACHJWA_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFO4310310.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            11237..11698
                     /gene="yjiG"
                     /locus_tag="ACHJWA_003797"
     CDS             11237..11698
                     /gene="yjiG"
                     /locus_tag="ACHJWA_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFO4310311.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            11711..12883
                     /gene="iadA"
                     /locus_tag="ACHJWA_003798"
     CDS             11711..12883
                     /gene="iadA"
                     /locus_tag="ACHJWA_003798"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFO4310312.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIVRAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            13005..13859
                     /gene="hypT"
                     /locus_tag="ACHJWA_003799"
                     /gene_synonym="qseD"
     CDS             13005..13859
                     /gene="hypT"
                     /locus_tag="ACHJWA_003799"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HFO4310313.1"
                     /translation="MTLEKCRNFSQAAVSRNVSQPAFSRRIRALEQAIGVELFNRQVT
                     PLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRKIKIAAAHSLSLGLLPSIIS
                     QMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLLEAPFDHIRLFESQLFPVCA
                     SDEHGEALFDLVQPHFPLLNYSRNSYMGRLINRTLTRHSELSFSTFFVSSMSELLKQV
                     ALDGCGIAWLPEYAIQQEIRSGQLVVLNRDELVIPIQAYAYRMNTRMNPVAERFWREL
                     RELEIVLS"
     gene            complement(13852..14244)
                     /gene="iraD"
                     /locus_tag="ACHJWA_003800"
     CDS             complement(13852..14244)
                     /gene="iraD"
                     /locus_tag="ACHJWA_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFO4310314.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSTLPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(14241..14324)
                     /gene="idlP"
                     /locus_tag="ACHJWA_003801"
     CDS             complement(14241..14324)
                     /gene="idlP"
                     /locus_tag="ACHJWA_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HFO4310315.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            complement(14440..14565)
                     /locus_tag="ACHJWA_003802"
     CDS             complement(14440..14565)
                     /locus_tag="ACHJWA_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310316.1"
                     /translation="MNNFPPELSSSGDKYFHLRNYSEYSEYTSGFFLSLMIFIKS"
BASE COUNT         3943 a         3559 c         3424 g         3640 t
ORIGIN      
        1 agtcatcact gcaatgttaa aaccgagcaa ctgcagcagt ttattaaaat aatggaacaa
       61 aaagcgcaac taccaatcta tattgacaag aacaatttga aagagagtat tttctctgtt
      121 ttgcacaatg acccacaaca agtagataaa gatcaacacc ttccctgtga aaagttttta
      181 aaacatgcct gcaaaagttc aaattcattt gaagtgaaat tagatgccac tcatcaatat
      241 caacacctga ataacttcat gatttctttt gacccagtag aaaatcaatt aaccatacgg
      301 gataacaata acaagactga aactttctcg tttacaaact tacaatggga aaatttgctg
      361 caatactaca aagaaaacca ccagcagcca aatatagcag gatcacgaaa tctcacggat
      421 aatatagata aaattaaaaa tacaatatcc acctctgaaa ttattgaatg cgcctctcct
      481 gaaataagaa gtagcgtcct gaacgatctt tatagcattg ctaatttcct cccggacaat
      541 aatctgaccc caaatgagag ctggaaaaga ttttgtgaga catgcgagcg cttttacgtt
      601 gctcagaaga gtatcactgg agataagagt gaacgtctta cgcgaaaact ctctatctct
      661 gatgcaggaa ttacaatgac cttcaagata ggtgatgttg tcatcaatac tattagcact
      721 gctattcctg aagatgcaac gggtcaacgg tgtatcgaag ggttgaattt agcagagatg
      781 gatttaaccg acatagactt gtcgaaaatg gcgctaagga atgtcaattt taatggcagc
      841 attcttagaa atgccaagtt ctccggtacg atctgtgaag gcgtggattt taccgattgt
      901 gatctgcgta atgcagaatt cgaaaatgcc tcattagaaa ataatgattt tcgtaaagtt
      961 cgccacttga cttatgtaaa tttcaaaaac gcaaatctac gaaacagtaa cttcaacgga
     1021 aaagttctca ctggcgtaac ctttactgga agtgacctta gtaacgcgta tcttgaacac
     1081 atagatttca caaccgtgat tctatatgaa acatctaaaa tacctggaat acctggaaca
     1141 cctcaaatac cgggaacacc taaagtaatt cttactggcg caatactaaa ttattccgat
     1201 ctatcgggaa aagatctttc agaatataat cttactggta ttctctgcat gtataccaac
     1261 ttttcaaacg ctaatttaac aaattgtaaa atctctaatg caaacttttc gaatgcaaaa
     1321 ttctacaata ctaattgtac tggtgcaaat tgttcgaata tcctatttga ctacgcatgg
     1381 tttgacaata caatatttat aaaaacgctt tttaaaaata cctgttttta caatgtcaga
     1441 gcgaaaaatg tctatcttga gggagcatat ctgaacaatg ataatatcgt gaatcaagcc
     1501 aataacagta ccgagaaaca atccattgac agtaccgata aacaggccaa tgacagtacg
     1561 gtgcaacaat ccattgacag tacggtgcaa caagccaatg acagtaccga taaacaagcc
     1621 aatgacaata tcgataaaca ggtcaatgac agtaccgata aacaagccaa gaacagtacc
     1681 gagcaacagg acagtaacag ttttaatcaa gcccgtttaa agaaagaagt gaataggaga
     1741 ttttccattc cgggtttaac gtcttatcag ccaacatata tagttgaaga atagttcatt
     1801 aatcacccaa acagagtggg tatggcagac gatatagatt aacatttaaa cgttaaccgc
     1861 ctcatctatc attcatagat gaggcgcatt tatcagtcga tgtaagctga aaatgttctg
     1921 ccatccagag acaaccgccg ccccctgcgg cgtttactat ttcatcgccc gcgttaagaa
     1981 ttcattaagc ttcatccgta tactccttat ctgtcgcact gaatttgacg agaaaaaagc
     2041 aataataagg agcaataagt tgaaaaagcg catcaccttt atcgtttttc tttgcgccat
     2101 cgtggcggca agcctgtttt ttgtgcaatc ctgcgtccgt aaaagccagc atgtcgcagg
     2161 gtttcagaat tatcaggcca ctatcgatgg taaagagatt acaggagtaa ctaaaaatat
     2221 ctcctcactg acctggtcag cgcagagcaa taccttattt agcactatca acaaacctgc
     2281 aaccatcgtt gaaatgacga ccgagggcga cttgatccgc accattccgc tcgattttgt
     2341 taaggatctc gagacgattg aatacattgg tgataataaa tttgtcatca gtgatgaacg
     2401 tgactacgcc atttatgtga tttcacttaa cgctgactcg gaagtgagca tcctcaaaaa
     2461 aattaaaatc ccattgcagg aaacaccaac taactgtggc tttgaagggc tggcttactc
     2521 ctctcaggac cataccttct ggttttttaa agagaaaaat ccgatagagg tctacaaagt
     2581 aaccgggcta ttgcgtagcg atgagttgca tatcagcaaa gataaaacgt tgcagcgtca
     2641 gtttacttta gacgatgttt ctggcgcgga atttaaccca cagaaaaata cgttgctggt
     2701 gttgtcacat gagtcacgcg ccctgcagga agtgaccgtg agaggtgacg tgatcggcga
     2761 aatgtcgtta accaaaggta agtatggcct ttcacacaac atcaagcagg cggaagggat
     2821 agcgatggat gatagcggca acatctatat cgttggcgaa ccgaatcttt tttatcgctt
     2881 tacgtcgaca aaatcacgat aacagtctgg caggttctcc ttttttgtta ttaacttgtt
     2941 aactcaaaat aatacaaacc aggcagaaca tgaattcgac atcgcatccc gtagaacgtt
     3001 tttctttcag caccgcgtta ttcgggatgc tggttctgac cttaggtatg ggtttaggcc
     3061 gctttctcta tacgccgatg ctgccagtca tgctggcgga aggcgagttt tcatttagcg
     3121 aactctcatg gatcgccagt ggtaactatg ccgggtatct ggcggggagc ctgctgtttt
     3181 cattcggcgc gtttcattta ccctcacgcc tgcgcccgtt cctgttagct tccgccctcg
     3241 caaccggatt attaatcctc gcgatggcgt ggctgccgcc gtttcttctg gttttcatca
     3301 ttcgctttct ggcgggagtc gccagcgccg ggatgttgat tttcggctca acactcatca
     3361 tgcaacatac tcgccatccc tttgtccttg cggcgctatt ttctggtgtt ggcgtcggca
     3421 tcgctctggg taatgaatat gtgctggcag gcctgcattt tgccctctct tcacaaacgt
     3481 tgtggcaagg tgccggagca ctttctgcca ttatattgct tgctctggcg ctgctcatcc
     3541 cgtcgaataa acacgttatc ccgccagcgc cattggcaaa aatcgcgcaa caacccatga
     3601 gctggtggtt actggcgatt ctgtatggtc tggcgggttt tggttatatc atcgtcgcca
     3661 cctacctgcc gctcatggcg aaagacgcgg gccagcctgt gttgacggct cacctctgga
     3721 cactggttgg cttgtcgatt gtcccaggtt gctttggctg gctgtgggca gccaaacggt
     3781 ggggagcatt accttgcctg accgcgaatt tgctggtgca ggcgatctgc gtgctgttaa
     3841 ccctcgccag cagctctcct ttattactca tcatcagcag tattggtttt ggcggcacct
     3901 ttatgggaac gacctcgctg gtgatgacca tcgcccgcca gcttagcgtg ccgggaaatc
     3961 ttaacctttt gggctttgtg acactcattt atggtatcgg gcaaattctt ggcccggcgc
     4021 tgaccagtat gctcggcaac ggaacgtcgg cgctcgccag cgccacactc tgcggcgcag
     4081 cggcgctatt tatcgcagca ttaatctgcg ggatgcaaat attcaaattg catacgaatt
     4141 attcttaagt aatttaacta atttaacttt tcagcacatc acgtaaaata attaaaatat
     4201 cctttttctt tggaggtact cagccaccgc cggtattatt cctggtttag caattccacg
     4261 gctaattcat tatttctggt catattatag tcaggatcgc attatatctc cgtgctagaa
     4321 aaaacaagtt taattctgtt ttttcacgga aggtcactat ggcttcgaca aatgcgcatc
     4381 aaataaaaaa taatgatcaa aactctttat gtggtctggg tgacaaaata cgccgcctga
     4441 ccgccggagt ttgtttattt acacaatttt ttttccccgt catggcgacg gcacaaaatg
     4501 tggtacatgc taaacctcag acgacagttt catctgcccc cccctctact agaaaataat
     4561 actgtgcctt acacgcttgg tgcgctggaa tcagcgcaaa gcgttgctaa tcgcttcggt
     4621 atttcactgg aggagcttcg tcgtcttaat cagttccgta cttttgctcg cggctttgat
     4681 aacgtgcgcc agggtgaaga actggatgtt ccggcaacaa cctcgcagaa aagccatgag
     4741 caacaaaatg ccgtaccgcc tgcgaatggc gaaaacacgc tggagaatca aatagccagc
     4801 acctcgcagc gagttggcac tctgctttca caagatatga atagtgagca ggccagcggc
     4861 atggcgcgtg gttgggcgtc ttcagaagcc tcaggcgcga tgactgattg gttaaataac
     4921 tttggtactg cgagaatctc tctgggtgtg gatgaagatt ttagcctgaa aaattcgcaa
     4981 ttcgacttcc tgcatccgtg gtatgacaca cctgattatc tgctcttcag ccagcatacc
     5041 cttcaccgaa cagacgatcg tacccagatc aacaccggtt tgggctggcg tcatttcacc
     5101 tccagctgga tgtcaggcat caaccttttt tttgaccacg acctgagccg ctatcactcc
     5161 cgcgcagggc ttggcgcaga atactggcgt gattatctga agttgagcag caacgcttat
     5221 atcggcctga ccggctggcg tagcgcacca gaattggata acgacttcga agcccgcccg
     5281 gccaacggct gggatttacg cgcggaaggc tggttacctg cctggccaca actgggggga
     5341 aaactggtct atgaacaata ctatggcgat gaagtggcgc tgtttgacaa gaatgatcgt
     5401 caaagtaacc cccatgctat taccgcaggc ctcaactata cccccttccc gcttctgact
     5461 ctcagtgcgg aacagcgtca ggggaagcaa ggtgaaaatg acacacgttt tgccgttgat
     5521 ctgacctggc aacccagcag ttcaatgcag aaacagctta atccggacga agtggccgga
     5581 cggcgcagtc tggccggtag tcgttatgac ctgattgatc gcaacaacaa catcgttctg
     5641 gaataccgca agaaagagct gattcgcctg agtctgctgg atccggtgaa agggaagtct
     5701 ggagaaataa aaccgctggt ttcctcgcta cagaccaaat atgcccttaa aggctataac
     5761 atcgaagccg ctgcgctgga agctgccgga ggtaaagtca gcacgtctgg aaaagatatc
     5821 acggtcacgc tgccaggtta ccgcttcact aacaccccag aaaccgataa tacatggtcg
     5881 atagacgtta ccgccgagga tgtaaaaggt aacctgtcac ggcatgaaca aagcatggta
     5941 gttattcagg ctccgacatt aagccagaaa gattctctgt tatccgtcaa tccgctaacc
     6001 gtggctgcag ataaaaaatc gacgaccaca ttgaccgtta ctgcgcacga ttccgacgga
     6061 actccggtgc cggggctggc gctgcaaacc cgcagtgaag gcgttcagga tatcaccctg
     6121 tctgactgga cagataacgg tgatggtagt tacacacaga tactgaccgc cggaacgaca
     6181 tcaggttcag taacactgac gccgcaaatt aacggtgaga gtgcggtaaa agaatccatc
     6241 gtcgttaata tcgtccctgt tgtctcatcc cgcgaccatt catcaataac aattgataac
     6301 gtatcgtatt atgccggaga cgacatcaag gttagggtgg aactgaaaga cgatagcaat
     6361 caaccggttg catatcaaaa agaggaattg gtaaaagccg ttactgtcga aaacagcaaa
     6421 cctggcgcca cgattgtctg gcacgaagag cagccggggg tttatgccgc gaattatccg
     6481 gcctataagc aagggactgc actaagggca caacttagcc ttcacaactg gaatgctcca
     6541 ctgcaatcgc atatttataa cattgaggca aaccagaata aggctcgcgt tgccacatta
     6601 tcagcgacaa ataatgacgt ttacgccgat aaaaagacat ttaataccct cacgatcaac
     6661 gtcactgatg agagtgataa tcccctgaca aatcatcagg tcacctttaa gaatgaaaaa
     6721 ggaagcgcgg agtttgtcga accgccgcag caaaatacgg atgcatatgg tgttgccaca
     6781 ataaacatgg taagtcaggt tgcggaagaa aatacgatta gcgccacgct gccaaatggt
     6841 ttttcacaac ggataattgc gaaattcgtt agcgattcga gtacgccaaa attcaaacaa
     6901 ctggttgccg atccagatac cattattgct ggcaacagcc agggcagtac tctgaccgcc
     6961 atcatcacag actttcataa caacccgtta aaagatatga aagtgaattt tgtggcacct
     7021 ggtggctcgc aactggacaa cacgaccgcc acaacagacc agtccggtat tgtgcgggtg
     7081 cacctgacca gttcaaaagc tggtagctat tccgtcgatg cctcgcttga ggtggataaa
     7141 aatattcacc agtcggtcac gatcaccgtg gtcccaaaca gggaacaatc ggtaatgacc
     7201 ttgaatgccg ggtcgggcag tgcgatcgct aacaatacaa atatcgttac cctgactgcc
     7261 agtgtgaaag atgtttatgg acacccgttg ccggatgagg atgtgaaatt taccttgcca
     7321 gcctccatga ccgggaactt cacgctaagt agtgaaaccg cccgcaccga tgcaaacggt
     7381 gatgccgtgg tcacattgcg aggcacaaaa gcgggtgagt ttacagttac ggcgacgctg
     7441 accagaaata ataccgttgc ttatcagcaa gtcactttta ttggggatac aaacagtgcg
     7501 cagctccagc cgctgactgc ctcattaaat tccattgttg cgggtaacag tacggggagt
     7561 accctgacgg caacgatcct ggacgcttac caaaatccgc ttaaagacca gttggtcact
     7621 ttccagagta acgatgtcac tctaagcgaa acagaagtca ccaccaatac gctgggtcag
     7681 gcgacggtaa caatgaccag caatattgcc ggacaacata acgtcgtggt gagccggaaa
     7741 gcgcaagctt ccgataataa aacgtttagt ttatcagtgc taccggatga aagttcggcg
     7801 aaggtaataa gtataaccgg agccgaaaaa acgataacgg tgggcgaaaa catcacgcta
     7861 cggatactcg tccaggacgc gtttaacaat gtaatcgcgg gtcaacgcgt cagattaagt
     7921 gcgcagccaa caactaacat tacgataggc gatacggctt acaccgataa taacggttat
     7981 gcgtacgtta accttctcag cacccaacct ggggtttatc aggtgacggc aacgctggac
     8041 aataacagta gtagtaaggt tgacgtgaat gtggcaaatg gcaaactcga gttaacatca
     8101 tcgaaaccag aaactacggt ccataatagt gagggtatta cgctgaccgc aacggcgaga
     8161 aatgcgcggg gtgaattgat gccagggcaa attatcacct ttagcgtaac gcctgaaggt
     8221 gcaacgctaa gcaatacagg ggaagtcctt actgaccagt caggtcaggc caaagtgacg
     8281 ctgaccagtg acaaagtgaa tgtctatacc gttacggcca taatgggcaa agatgttccc
     8341 gttcagagcc aggtaacggt tgcggttaag gcagatgcta aaacggcaca tgttgtgagc
     8401 gtcgtggctt ctcctgacac catcaccgcc gacggcatcg atagcagcac catcacttca
     8461 cgagtagaag atgattacgg attcccggtt gaaggtgtcg atattagtca tggcttagac
     8521 accaaaggca gcccggtagt taatattcca actacgcgta ccgatcagtc cgggcaagtc
     8581 acggcgacaa taaccagtac attggcagaa accttaacag tcaatgtgca agttcctggc
     8641 acagccaacc aatccgcaac cattacattg gttgccggca cggccgatga aagtaagtca
     8701 attttgaaat ccgatgttga cactctgaag gctgactacc agcagagcgc aaaacttacg
     8761 ctaacattgc aagacaagta cggtaacccg atagtgacgt ctgatcatct ggaatttgtc
     8821 cagtcaggcc ccttcgtgaa ctttctcaag ttgagcgata ttgattacag ccaaagaaat
     8881 tatggcgagt acaccgtgac tgtcactggc ggaaaagagg gaacagcgac actcattccc
     8941 atgctgaacg gggttcatca ggcaaactta agcatatcgc tgaatctcat ccaatcgata
     9001 aaagaaatgt ccggtcatgt cactgcaaac aaccatacct tctccacggc taaattcccg
     9061 agcgaaggct ttgcaggagc gtattacaca ctcaacaatg ataactttga agcgggtaaa
     9121 accgttgatg attatatgtt ttcaagttca cagggttggg tgtctgtcga tgcttcgggt
     9181 aaagtttctt tcgcaaatat cggcgatcaa acgtcagtca caataagcgc tgttccccga
     9241 caaggaggta caacctacca gaccttaatt aagctgaaag gctggtgggt gaataatgga
     9301 aatcatacca atatctggct agctgccaat gcgctctgtc atgctaaaaa tgatggatat
     9361 aatcttcctg gcatcacaca tttgacgtct ggcgaaaaca aacgcacgca gggatcactg
     9421 tatggtgaat gggggaacgt tggagcgttt tccagtaatt cgcaatttac accgggagct
     9481 tactggacaa gtgaatctga tgattacagt cggcactact atgtgcagat gctaaccggt
     9541 atgaccggaa gcgacgctga ttccagcccc caactgaccg cctgccgtaa atcactttaa
     9601 tttcagcagt tgttattaaa ggataccctt ttacgagggt atcctttcag actgataaca
     9661 aacgctacat tgccggatgt gctgcgctta tcaggcctat ataacccctg caatatattg
     9721 aatttattgt ttttgtaccc cagccggcat aaacgactac tcaagaaact caacagcaac
     9781 cgttgatgtc agccagaccc cgttttccgc ttgccagaaa gggatgcctc gtttcgccat
     9841 ttcctgtgct ttgacggtta agacaaccgg cgatccgtgt cttgctccca ctttgcgtgc
     9901 cgttgcttca tcagcagaga gatgcacgta atgacgatcc cctgcaatca gcccctgttt
     9961 ttttatctcg tcaaggaaac ggcttgccgt accgtggtag agaaactgtg gcggtgtttt
    10021 ttcggcaaag gaaatagcga cctgcgaagt cgaatgcccc tgaaccgcgc ggatgcacct
    10081 accatcactg gaataactaa aacgctttta tcgctggttg cgaccacggt atccagcaat
    10141 gcacgggtta atcgcttgcc cgctttttgg gcacaaagta tgagcttatc gatattcgcc
    10201 catccttcac ggtccagtac gataccaatc gcctccggtt tgtgtcggag gacaaaactt
    10261 aaaaacttgc tcgtatccgc cagctctttt tcgttgtact ttgccatcgc gttttcgtcc
    10321 ctggattttc tgtttttatt taccaatgtc attgtcactg ggtttatgaa ttttctgcaa
    10381 ctcaatcctg cgcagaacct tttccacaca gcgtaatttt tcccctatgc aaaaaacgca
    10441 tagccagaaa catacttgca ttttccccat acccacatgg ctgataaggt ttttaagttc
    10501 caggaatttt cctggctcaa gcaacacaca aacacaaaaa aacaatgggg attgttatga
    10561 cacaacaagg ggatgctgta gccggggagc tggcgacaga aaaagtgggc attaaagggt
    10621 atctggcttt ttttctcacc ataatctttt tttccggtgt tttttccgga acagacagct
    10681 ggtggcgcgt ttttgatttt tccgttctga acggatcgtt tgggcaactg cctggtgcga
    10741 atggagccac cacctcattt cgcggcgcgg gtggcgcggg ggctaaagat ggctttctct
    10801 ttgcgctgga gctggcaccg tcagtcattc tctctctggg gattatttca attaccgatg
    10861 gtcttggtgg attacgcgcc gcgcagcagt taatgacacc tgtgctaaaa ccgttgttgg
    10921 gcattccagg gatctgctcg ctggcgctga tcgctaactt gcaaaacact gatgccgccg
    10981 ccggtatgac caaagagctg gcgcaggaag gtgaaattac cgaacgcgat aaagtgattt
    11041 tcgccgcata tcaaaccagc ggtagcgcca tcatcaccaa ctacttctct tccggcgtcg
    11101 cggtgttcgc ctttctgggc acgtccgtaa tcgtaccttt agccgtcatc ctggtgttta
    11161 aatttgtcgg agccaacatt ttgcgcgtct ggcttaactt cgaagaacgt cgcaacccaa
    11221 cgcaaggagc gcaagcatga caactcaggt ccgcaaaaac gtcatggata tgtttatcga
    11281 tggcgcgcgc cgtggcttta ctatcgccac gaccaacctg ctgccaaacg tggtgatggc
    11341 gtttgtcatc attcaggcgc tgaaaattac cggcctgctc gactgggtgg ggcatatctg
    11401 tgaacccgta atggcgctgt ggggattacc cggtgaagcg gcaaccgtcc ttttagccgc
    11461 attaatgagt atgggcggcg cagtcggtgt tgccgccagt ctggcaaccg ctggcgcatt
    11521 aaccggtcac gatgtcaccg ttttactgcc cgcgatgtat ttaatgggca acccggtgca
    11581 aaatgtcggt cgctgcctgg ggacagccga agtgaatgcc aaatattacc cgcatatcat
    11641 tacggtctgc gtgattaacg cattgctgtc gatctgggtt atgcagctta ttgtttaaca
    11701 aggagttacc atgattgatt ataccgcagc cggttttacc ctgctgcagg gagcgcattt
    11761 gtatgcgccg gaagatcggg gaatttgcga tgtcctcgtc gctaacggca aaattatcgc
    11821 cgttgccagt aatatccctt ctgacattgt accggactgc acggttgtcg atctcagcgg
    11881 gcagatcctc tgcccaggtt ttattgatca acacgtccat ttgattggcg gtggcggcga
    11941 agcaggtccc acgacgcgca cgccggaagt ggcgctaagt cgcctgacgg aagcgggcgt
    12001 cacgtcagtg gttggtctgc tgggcaccga ctctatctct cgccacccgg aatccctgct
    12061 cgccaagacc cgtgcgctca atgaagaagg catcagcgcc tggatgctga ccggcgctta
    12121 tcatgtccct tcccgcacca ttacgggttc cgtggaaaaa gacgtggcga ttatcgatcg
    12181 tgtgattggc gtgaaatgtg ccatctctga tcaccgttct gccgcaccgg acgtttatca
    12241 cctggctaat atggcggcag aatcccgcgt tggcggtttg ctcggcggta aacctggcgt
    12301 caccgtgttc cacatgggcg acagtaaaaa ggcattacag cctatttatg acctgctgga
    12361 aaactgcgat gtgccgatca gcaagctgct gccgacccac gttaaccgca acgtaccgtt
    12421 gtttgagcag gcgctggagt tcgcgcgcaa aggcggcacc atcgatatca ccagcagcat
    12481 tgacgaaccg gtcgcccctg ccgaaggtat tgtccgcgcc gttcaggcgg gtattccgct
    12541 ggcacgcgtc accctcagct ccgacggcaa cggtagccag ccgttcttcg atgatgaagg
    12601 gaatttaacc catatcggcg ttgcaggttt tgaaacgttg ctggaaaccg tgcaggtgct
    12661 ggttaaagac tatgatttca gtatcagcga tgccctgcgc ccgctcacca gtagcgtagc
    12721 cggtttcctt aacctgaccg ggaaaggcga aattctgcca ggcaatgacg cagacttact
    12781 ggtcatgacg ccagaattgc gcattgagca ggtatacgct cgcggcaaac tgatggtcaa
    12841 agacggcaaa gcctgcgtga aaggaacgtt tgaaacggct taaacggttg tcagccgccc
    12901 taatggacgt agaatgcccc caagggcggc tgacagagta aaacgtaatg gatgactgtg
    12961 gtgcggtttt gcataatatt gaaaccaaat ggctttaaga ttttctgacc ctggaaaaat
    13021 gccgcaattt ttcccaggcg gcagtcagtc gtaacgtctc gcaaccggca ttcagccgcc
    13081 gcatccgcgc gctggaacag gcgattggcg ttgaattgtt taaccgccag gtgacgccgc
    13141 tgcaactctc agaacaaggg aaaatcttcc attcgcagat ccgccatctg ttgcaacagt
    13201 tagaaagcaa ccttgcagag ctgcgtggcg gcagcgatta cgcgcaacgt aaaatcaaga
    13261 tagccgctgc acactctctt tccctcggtc tgttaccgtc cattatcagc cagatgccgc
    13321 cgctctttac ctgggcgatt gaagccattg atgtcgatga agcggtcgat aaactgcgtg
    13381 aaggacaaag tgactgtatt ttttcctttc acgacgaaga tttgctggaa gcgccgtttg
    13441 accacattcg cttatttgaa tctcaattgt tccctgtctg cgccagtgac gaacatggag
    13501 aagcactttt tgacctcgtg cagccacact ttccgttact gaattacagc cgcaactcct
    13561 acatggggcg attgattaat cgcaccctga cgcgccacag tgagttaagt ttcagcacct
    13621 tttttgtctc ttcgatgagc gagcttttaa agcaggttgc cctcgacggc tgtgggattg
    13681 cctggctgcc ggagtatgcc atccaacaag aaattcgcag cgggcagctc gttgtgctga
    13741 atcgggacga actggtgatc ccgattcagg cctacgcata ccggatgaat acccgcatga
    13801 atccagttgc cgaacgcttc tggcgtgaac tgcgcgagct ggagattgtg cttagctgac
    13861 attctcaagc gtcgcactgc gccaaccgcc attatcccag accagatcaa acgttagcgg
    13921 ttcatcgtcg tggtacagca ttatctcaag gctgatcgcc agagtgtggc agtacgcttc
    13981 tttcaaatta acctgcaacg ccgcaatccg tggctcatga aataacagtg cagaacgcag
    14041 ctgacggcaa taccagctct ggagatgttt gtcgctcggg ttatgcactt gccactcggg
    14101 catgcctgaa acaagtaatg acggtaatgt cgaatgagga gaattaaaaa gagtgaggag
    14161 atcgctgcat accgatttac gcagcaatga cgttttattg cctgaaattt caggtaaaac
    14221 agcctgaagt gattggcgca tcattttgcg cactcctgac gtttagcaac atatgcggct
    14281 tgccctgaac accgggcacc actgtattga tgctcatttt ccatagccat tcccttttgt
    14341 tcattacatc catgtcagtc tgagacaggc ctgaatcctg tcagcaaagt ataaaacctt
    14401 acttgcctat gaatatctac tgacttttca ggaaaaaagt catgatttga taaaaatcat
    14461 caaactcaaa aaaaaaccgc tagtatattc tgaatattcc gaatagttac gtagatgaaa
    14521 atacttgtca ccgctgctac ttaattccgg tgggaaatta tttatt
//
LOCUS       DAZFAB010000108        14477 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZFAB010000108 DAZFAB010000000
VERSION     DAZFAB010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14477
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.108"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(130..882)
                     /gene="tcyN"
                     /locus_tag="ACHJWA_003803"
     CDS             complement(130..882)
                     /gene="tcyN"
                     /locus_tag="ACHJWA_003803"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFO4310317.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARKLLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPEQPRTRQFLEKFLLQ"
     gene            complement(879..1547)
                     /gene="tcyL"
                     /locus_tag="ACHJWA_003804"
     CDS             complement(879..1547)
                     /gene="tcyL"
                     /locus_tag="ACHJWA_003804"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFO4310318.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     TAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(1562..2548)
                     /gene="dcyD"
                     /locus_tag="ACHJWA_003805"
     CDS             complement(1562..2548)
                     /gene="dcyD"
                     /locus_tag="ACHJWA_003805"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFO4310319.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASVEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(2653..3453)
                     /gene="tcyJ"
                     /locus_tag="ACHJWA_003806"
     CDS             complement(2653..3453)
                     /gene="tcyJ"
                     /locus_tag="ACHJWA_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO4310320.1"
                     /translation="MKLAHLGRQALMGVMAVALIAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(3541..4092)
                     /gene="fliZ"
                     /locus_tag="ACHJWA_003807"
     CDS             complement(3541..4092)
                     /gene="fliZ"
                     /locus_tag="ACHJWA_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFO4310321.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRISLVRDGKIV
                     NKIEISRLDTLLDENGWQTEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(4138..4857)
                     /gene="fliA"
                     /locus_tag="ACHJWA_003808"
     CDS             complement(4138..4857)
                     /gene="fliA"
                     /locus_tag="ACHJWA_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFO4310322.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(5177..6934)
                     /locus_tag="ACHJWA_003809"
     CDS             complement(5177..6934)
                     /locus_tag="ACHJWA_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliC/FljB family flagellin"
                     /protein_id="HFO4310323.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQATTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGETITIDL
                     KKIDSDTLGLNGFNVNGKGTITNKAATVSDLTSAGAKLNTTTGLYDLKTENTLLTTDA
                     AFDKLGNGDKVTVGGVDYTYNAKSGDFTTTKSTAGTGVDAAAQAADSASKRDALAATL
                     HADVGKSVNGSYTTKDGTVSFETDSAGNITIGGSQAYVDDAGNLTTNNAGSAAKADMK
                     ALLKAASEGSDGASLTFNGTEYTIAKATPATTTPVAPLIPGGITYQATVSKDVVLSET
                     KAAAATSSITFNSGVLSKTIGFTAGESSDAAKSYVDDKGGITNVADYTVSYSVNKDNG
                     SVTVAGYASATDTNKDYAPAIGTAVNVNSAGKITTETTSAGSATTNPLAALDDAISSI
                     DKFRSSLGAIQNRLDSAVTNLNNTTTNLSEAQSRIQDADYATEVSNMSKAQIIQQAGN
                     SVLAKANQVPQQVLSLLQG"
     gene            7200..8597
                     /gene="fliD"
                     /locus_tag="ACHJWA_003810"
     CDS             7200..8597
                     /gene="fliD"
                     /locus_tag="ACHJWA_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFO4310324.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSSTTAFSATTAGNAIAGKYTISVTHLAQA
                     QTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNAKAG
                     VSASIINVGNGDYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEVSVA
                     AQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIKDWV
                     NAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVSSSS
                     YKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGSNLT
                     SWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSLNST
                     SSYLTQQFENNSNSK"
     gene            8622..9032
                     /gene="fliS"
                     /locus_tag="ACHJWA_003811"
     CDS             8622..9032
                     /gene="fliS"
                     /locus_tag="ACHJWA_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFO4310325.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVETLMRNIADAWKESLLSPSLIQDPV"
     gene            9032..9397
                     /gene="fliT"
                     /locus_tag="ACHJWA_003812"
     CDS             9032..9397
                     /gene="fliT"
                     /locus_tag="ACHJWA_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFO4310326.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVDPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            9475..10962
                     /gene="amyA"
                     /locus_tag="ACHJWA_003813"
     CDS             9475..10962
                     /gene="amyA"
                     /locus_tag="ACHJWA_003813"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO4310327.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKVQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPNEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRNYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVL"
     gene            complement(10996..11409)
                     /gene="yedD"
                     /locus_tag="ACHJWA_003814"
     CDS             complement(10996..11409)
                     /gene="yedD"
                     /locus_tag="ACHJWA_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFO4310328.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            11596..12801
                     /gene="yedE"
                     /locus_tag="ACHJWA_003815"
     CDS             11596..12801
                     /gene="yedE"
                     /locus_tag="ACHJWA_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFO4310329.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVAAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            12798..13031
                     /gene="yedF"
                     /locus_tag="ACHJWA_003816"
     CDS             12798..13031
                     /gene="yedF"
                     /locus_tag="ACHJWA_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFO4310330.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            13140..13808
                     /locus_tag="ACHJWA_003817"
     CDS             13140..13808
                     /locus_tag="ACHJWA_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFO4310331.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAREWMRQEISGKEASEIAASGCVPANQFSWHPVSRAVGNVKNQGAEL
                     IQPV"
     gene            13919..14398
                     /locus_tag="ACHJWA_003818"
     CDS             13919..14398
                     /locus_tag="ACHJWA_003818"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO4310332.1"
                     /translation="MFTIKTDDLTHPAVQALVAYHISGMLQQSPPESSHALDVQKLRN
                     PTVTFWSVWEGEQLAGIGALKLLDDKHGELKSMRTAPNYLRRGVASLILRHILQVAHD
                     RCLHRLSLETGTQAGFTACHQLYLKHGFVDCEPFADYQLDPHSRFLSLTLCEDNELL"
BASE COUNT         3527 a         3706 c         3607 g         3637 t
ORIGIN      
        1 aattactttt gcaagccatc gcattctctt atgttattaa tgagttatgc tgatttttta
       61 agcagtttta tcaggcttga aatggcgttc cgccccgact gtcgaatcgt cggggctgat
      121 tttttcttat tattgcagca gaaacttctc gaggaactgg cgggtgcgag gctgctcggg
      181 atcggcaaat aacgcttttg cggccccctg ctcgactatc cgcccctggt ccataaaaat
      241 cgcccggtcc gcaacatccc gggcaaagct catttcgtgt gtcacaatca ccatcgtgcg
      301 cttttcctgc gccagctgac ggatggtgtt caggacttcg cccaccagct ctggatccag
      361 cgctgacgtt ggctcgtcaa acaaaatcac ctcaggacgc attgccagcg cacgcgcaat
      421 cgcaacacgc tgctgttgac cgccagacaa acgacgtgga tagctggttt ctttaccggc
      481 cagcccaact ttcgccaaca gcttgcgagc gcgcgccgtg gcctcttctt tcggttcgcc
      541 tttgacgatc accggccctt caataatgtt ctccagcacc gtacgatgcg gaaacaaatt
      601 aaagttctgg aagacaaacc cgacgtgctg acgcaactgg cgaatcagcg atttttgctg
      661 acttaatgaa cgtgcagtat caatagtgat atcgccaacg gtgatcgttc ccgcttccgg
      721 ttgttccagc agatttatgc tacgtagcaa tgtggttttg ccggaaccac tcggaccgat
      781 aattgccacc acttcgccag gctttacctc gaggtcgata ccgtgcagca ccgtctgacc
      841 gtggaatttt ttcactaggt tcttaacttc aatggcactc atttgggttc tctctcctgg
      901 cgattaagtt gattctcaaa atggttctgc aacgtcgata acacagtcgc catgatccag
      961 tagatgagcg aagccgccag atacatggtg aatacctcca gcgtacgcga ggtaatcaac
     1021 tgcgcctgac ggaacagctc cggcacctgg attgtcgcgg ccagcgacgt atcttttacc
     1081 agactgataa agctgttcga gagcggcggc aacgccaccc gcgccgcctg tggcaaaatc
     1141 gcgcgacgca tagtctgcca tggcgtcata ccaatactgg cagctgcttc ccactgacct
     1201 ttatcaatag aagaaatggc ggcgcgaagc gtttcggcag tataggcggc agtgttgagc
     1261 gacagaccaa tcattgctga cggaatggga tctaattcga taccaaactg cggcaggccg
     1321 taatagatca taaacagttg tgcgatgagc ggcgtaccac gaaaaataga gatataaaaa
     1381 cgcgccagcc agcgcacggg ccagattggc gacaggcgca tcagcgcgag aataaaccct
     1441 agcagtaaac caaaaaacat gccgccgata ctgagttgca gcgtataccc agcccctttc
     1501 agcaggaacg gcaaggaatc aataaccagt tgtatacttt cttgcatgag cgttcccgga
     1561 gctaaacgtg gggatgatag gcgaacagcg caggcgcgcc gccggtatga ataaacagaa
     1621 tcggcccttc atctttgaag cgtttctgac tgataccgtc aatcagaccc gccatcgctt
     1681 ttccggtata cacaggatca agcagaatgc cttccagccg cgccagcaat ttcactgctt
     1741 ccatgccttc gtcgttcggc acgccgtagc caggtgcaaa atagtcatcc cagagtaaaa
     1801 tttccactga tgcggtcagc tccagttctt tcgcaatcgc ctgttgtagg ttaaccactt
     1861 tcggcaattg atcggcaacg gaacgcgaca cggtcacgcc aatcagttcg ctttcaggca
     1921 tcaggtgttc cagcccaaca gccagtccgg cgtgagttcc ggcactgccc gatgcgacta
     1981 ccaccgatga aatattaacc gccccttcac actgttgcgc gatttccagc gcactctcca
     2041 cataacctag cgcgccaaga gcattagaac cgccaaccgg aatgacatac gggcgaaagc
     2101 cttgtgcttc gactcgcgtc gccagctctt ccagttgggc attgggatcg gtcagtgcgt
     2161 cgcacatttc aatctgggta ttgaacagat ccagcaacaa gcgattgccg ttggttaaat
     2221 agttttctgc ggttgtgcca ataggatttt ccagcagcgc cacgcagtgc agaccgagtt
     2281 tggcagcgac tgcggcagtc tgccgcacat ggttagactg gatcgccccg gcagtaatca
     2341 gcgtatcggc accttcacgc agagcatctg ccgcgagaaa ttctagctta cgtaatttat
     2401 tgccgcccat tgccatgggg gtgacgtcat cccgtttgat gaaaatttcc cgtcctagat
     2461 aatcagaaaa gcgcggcaga tattcgagcg gcgttggcgc gccgataaac tccagccgtg
     2521 gaaaacgggt taaattatgc agtggcataa cagcctccga tgtgtgttgt tgtgattttc
     2581 tcattatgca cgctgaaaac gcgtaaataa aaaaggcgct agtgaaagcg cccttttttg
     2641 tcattatgct gattatttgg tcacatcagc accaaaccat ttttcggaaa gggcttgcaa
     2701 agtgccgtct ttttgcattt ccgcaattgc atcattcact gctttcagca ggtcttcatt
     2761 tcctttacgc agcgccacgc cagactcctg acgggagaat gcttcaccgg ttactgccag
     2821 cgtatcgttg gttttcttca ccagatccag cgccgccaga cgatcaacga ggatcgcatc
     2881 gatacgccct acgcgcagat cctgatattt ggtcgggtca tcatcatagg tacgcacatc
     2941 gacgccctga acattctgcc gcagccactc ttcatagttg gtgcccagac cgacccccac
     3001 ttttttgcct ttcagatcat cggctgtttt aatggtgcct tcgttacctt ttttcaccag
     3061 cgcctgaata ccagaaatgg tgtacggggt tgagaaatcg tattttttct tgcgctcatc
     3121 agaaatggtg acctgattaa tcaccacatc aatacgttta gagtccagcg acgccagcat
     3181 accgtcccat ttggtcggtt ttagtgacgc ctcaacgcca agatgttttg ccagctgttg
     3241 ggcaaattcc acttcaaaac cggttaattt gccgtcatcc ccctgaaaac tgaacggcgg
     3301 ataagtacct tccagcccta ccagcagcgt gccgcgctct ttaactttat taagcagacc
     3361 ttcatctgca aaacttttaa cgctcatgcc cgcaatcagc gctacggcca tcacacccat
     3421 caatgcctga cgtcccagat gtgctaattt catattcacc ccgaatgttg ttatgtctgt
     3481 ttgcagtgta gagccattgt ttgtaaacac aaaaacaact ccgctacatc ttattcttat
     3541 ttaatatata tcagaagcag gcaggctgga ggatttctgc acaagtccgg tacaggtttg
     3601 gcgctgatag tgttgatact tccgtaacgc gatgcggtaa ttgttggtgc tggtggtggg
     3661 cagccacggg gcgaggattt catcaaggaa accgtcctgc agctgatcga gggaaatatt
     3721 ttgctcgtgt agatgattcc ccaaacggcg cagacgaacg acatattcac gcaccgtacc
     3781 agggctcatt tcagtttgct caaaaagaaa ttgcttaaag ccgataatat cgaaaaaatc
     3841 actctgcgtt ttgcaatgta aatcaccgca aaatcggcac aatgccgccc atgatttttg
     3901 ttccgtttgc cagccatttt catcaagcag cgtgtccagg cgggaaatct cgattttatt
     3961 cactattttg ccgtcgcgaa ctaagctaat gcgatcgagc aatttacggc aatgcgcgca
     4021 atgggtctgg ctgtgtttaa agtctttaag atagcggctt aatggccgtc ttttcaggtg
     4081 ctgcaccatc attaagaact cctggtagtc aaagttaaag tgcggcattt actgacgtta
     4141 taacttaccc agtttagtgc gtaaccgttt aatagcctgg ctgtgtaact gactgacccg
     4201 cgattccccg acctccagca ctgcaccaat ctctttgaga ttcagctctt cctggtaata
     4261 gagggttaat accagttttt cgcgctccgg caacgtttcg atagcttcca tcacccgctg
     4321 gcgcagatta ctgtccagta gttgttgtag cgggttttct cgttgatgat catcagtaac
     4381 caattcgatg ctatcgccgt gctcttcgcg ccactcatcg taggagaaga gctggctgtt
     4441 attggtgtcg agcaacattt ggcgataatc ggcaatatcg atccctaaac gttccgctac
     4501 ctcagtttcc gtggcgttgc ggccaagttc ctgctccagt tgccctattg cctgtgccac
     4561 ttcacgcgcg ttgcgtcgca cgctgcgcgg cacccagtca cggctgcgaa gttcatccag
     4621 catagcgcca cggatacgct gcactgcgta agttgtaaat gccgttcctt gtagggcgtc
     4681 atagcgttcg acggcattaa gtaacccaat gccgcccgcc tgtagcagat cgtcaagttc
     4741 cacgctcgcg ggcagtcgaa cctgcaggcg caatgcttcg tgacgcacca gcgggacata
     4801 acgctgccac agcgagtgtt tatccattac accttcagcg gtatagagtg aattcacgat
     4861 aaacagccct gcgttatatg agttatcggc atgattatcc gtttctgcag ggtttttaat
     4921 cggacgatta gtgggtgaaa tgaggggtta tttgggggtt acaggtaaat tccaggcaga
     4981 aaaaaccccg ccggtggcgg ggaagcacgt tgctgacaaa ttgcgcttta tgttgccgga
     5041 tgcggcgtaa acgccttatc cggcctacaa gaatgtgcaa attcaataaa ttgcaattca
     5101 acttgtaggc ctgataagcg cagcgcatca ggcaatttgg cgttgccgtc agtctcagtt
     5161 aatcaggtta caacgattaa ccctgcagca gagacagaac ctgctgcggt acctggttag
     5221 cttttgccag cacggagtta ccggcctgct gaatgatctg cgctttcgac atgttggaca
     5281 cttcggtcgc atagtcggcg tcctgaatac gggactgcgc ttcggacagg ttggtagtgg
     5341 tgttgttcag gttggtgact gcggaatcca gacggttctg gatagcaccc agggaagaac
     5401 ggaatttgtc gatggagctg attgcgtcgt ccagggcagc aagcgggttg gtcgttgcag
     5461 aaccagcact ggtagtctca gtagtgattt tacccgcgga gttcacattt acagcagtac
     5521 caattgctgg agcataatct ttattggtat cagtcgctga agcatacccg gcaacagtca
     5581 cagagccgtt atccttgtta acgctgtaag agactgtata gtcggcaacg ttagtgatac
     5641 cacctttatc atccacataa gacttcgcag catcactgga ttcacccgcg gtaaacccaa
     5701 tagttttgct cagtacaccg gaattaaagg taattgaaga tgtcgcggca gccgctttgg
     5761 tttcgctcaa tactacatct ttactcactg tagcctgata agtaatccca ccagggatta
     5821 acggagctac tggagtggtt gtcgcaggag ttgcttttgc gatggtatat tctgtgccat
     5881 tgaatgtcag agaggcaccg tcactacctt cgctcgctgc tttgagcagc gctttcatat
     5941 cagctttagc tgcgctacca gcgttgttag tcgtcaagtt gcctgcatcg tctacgtatg
     6001 cctggcttcc accgatggtg atattacctg ctgaatccgt ttcgaaagaa acagtaccat
     6061 cttttgtggt gtaagaacca ttaacagatt tacccacatc agcatgaagg gtggcagcta
     6121 acgcatcacg ttttgaagct gaatcagcag cctgcgccgc ggcgtctaca cccgtaccag
     6181 cagtagattt agtggtagta aaatcaccag atttagcgtt gtaagtataa tctacgccgc
     6241 caactgtgac tttatcgcca ttccctaatt tatcgaatgc agcatcggta gttaacaagg
     6301 tattttcggt tttcagatca taaagacctg tcgtggtgtt taacttcgcg ccagcagaag
     6361 ttaaatcact taccgttgca gctttgttgg taatagtacc tttaccattt acgttaaagc
     6421 cattcagacc cagagtatca gaatcgattt ttttcaggtc gatcgtgatg gtttcgccgt
     6481 cattcgcacc aacctgaatt ttcattgaac cgtctttcgc cagcacgttc acgccgttga
     6541 actgggtctg gccggatacg cggtcaattt catcaagacg agatttgatt tcgtcctgga
     6601 tggagtccag gtcagaatcg gagttagtcc ctgtagtggc ctgaaccgtc agttcacgaa
     6661 tacgctgtaa gttgttgttg atttcggaca gcgcgccttc ggtggtctgc gcaacagaaa
     6721 taccgtcgtt ggcgttacgg gccgcctgag tcaggccttt aatgttagaa gtaaaacggt
     6781 tagcaatcgc ctgacctgcg gcgtcatcct tcgcgctgtt aatacgcaag ccagaagaca
     6841 gacgctcgat agaactcgac agcgcagact ggttcttgtt gatattattt tgagtgatca
     6901 gcgagaggct gttggtatta atgacttgtg ccatgattcg ttatcctata ttgcaagtcg
     6961 ttgattacgt attgggtttc cacccgtcgg ctcaatcgcc gtcaaacctg ttatcgtctg
     7021 tcgtaaaaca acctttagaa tttttttcac aaacagccat tttttgttag tcgccgaaat
     7081 actcttttct ctgcccctta ttcccgctat taaaaaaaac aattaaacgt aaactttgcg
     7141 caattcaggc cgataacccc ggtattcgtt ttacgtgtcg aatgataaaa ggaaatcgca
     7201 tggcaagtat ttcatcgctg ggagtcgggt caggtctgga tttaagttca atccttgata
     7261 gcctcaccgc cgcgcaaaaa gcgacgctaa cccccatttc aaatcagcaa tcgtcgttta
     7321 ccgctaaact tagcgcctac ggtacgctga aaagcgcgct gacgactttc cagaccgcca
     7381 atactgcatt gtctaaagcc gatctttttt ccgccaccag cagcaccacc gcgttcagtg
     7441 ccaccaccgc gggtaacgcc atcgccggga aatacaccat cagcgtcacc catctggcgc
     7501 aggcgcaaac cctgaccacg cgcaccacca gagacgatac gaaaacggcg atcgccacca
     7561 gcgacagtaa actcaccatt caacaaggcg gcgacaaaga tccgattacc attgatatca
     7621 gcgcggctaa ctcgtcgtta agcgggatcc gtgatgccat caacaacgca aaagcaggtg
     7681 ttagcgcgag cattattaac gtgggtaacg gtgattatcg tctgtcagtc acatcaaatg
     7741 acaccggcct tgacaatgcg atgacgctct cggttagcgg tgatgatgcg ctacaaagtt
     7801 ttatgggcta tgacgccagt gccagcagca acggcatgga ggtctcggtc gccgcccaga
     7861 atgcgcagct gacggtcaac aacgtcgcca ttgagaacag cagcaacacc atcagcgacg
     7921 cgctggaaaa catcaccctg aacctgaacg atgtcaccac gggcaaccag acgctaacca
     7981 tcactcagga cacctccaaa gcgcaaacgg cgattaaaga ttgggtgaat gcctataact
     8041 cgctaataga taccttcagc agcctgacca aatacaccgc cgtagatgcg ggagctgata
     8101 gccagagttc tagcaatggc gcactgctcg gcgactccac gctgcggacg attcagacgc
     8161 agttgaagtc gatgctgagt aataccgtca gttcttccag ctataaaacg ttggcgcaga
     8221 ttggtatcac gaccgatccc agcgatggca aactggaact ggatgccgac aaactcaccg
     8281 ctgcactgaa aaaagatgcc agcggcgtag gtgcattgat tgttggcgat ggtaaaaaaa
     8341 ccggcatcac gaccaccatc ggcagcaacc tgaccagttg gctttcgaca acgggcatta
     8401 ttaaagccgc taccgatggc gttagtaaga ccctgaataa attaactaaa gactacaacg
     8461 ccgccagcga tcgcattgac gcgcaggtcg cgcgctacaa agaacaattt acccaactgg
     8521 acgttttaat gacctcgtta aacagcacaa gcagctactt aacgcagcag ttcgaaaaca
     8581 acagtaattc caagtaatca ttattcatcg ggagacaggt catgtacgcg gcaaaaggca
     8641 cccaagccta tgcacaaatt ggcgtcgaaa gcgccgtaat gagcgccagc cagcagcagc
     8701 tggtcaccat gctatttgat ggagtgctga gcgcactggt tagagcgcgc ctgtttatgc
     8761 aggacaacaa tcagcaaggc aaaggcgtct ctttgtcaaa agcgatcaac atcattgaga
     8821 acggactgcg ggtgagtctt gatgaagaga gcaaagacga actaacccaa aacttgattg
     8881 ctctttatag ctatatggtc aggcgtttgc tgcaagccaa tttacgcaac gatgtctccg
     8941 cagtcgaaga agtggaaaca ttaatgcgca atattgccga tgcctggaaa gagtcgttac
     9001 tctccccttc tttgattcag gacccagtct gatgaaccat gcaccgcatt tatatttcgc
     9061 ctggcaacaa ctcgtcgaaa aaagccagct catgttacgc ctggcaacgg aagaacaatg
     9121 ggacgaactc atcgccagcg aaatggcgta tgttaatgcg gtgcaggaga ttgcacattt
     9181 gactgaagag gttgacccgt ccaccacgat gcaggagcag ctccgcccga tgctgcgcct
     9241 gattctcgac aacgaaagca aggtaaagca gttattacag attcggatgg atgaactggc
     9301 gaaactggtc ggtcagtcat cggtgcaaaa atcggtgttg agtgcctatg gcgatcaggg
     9361 cggctttgtg ctggctccgc aggataacct cttttgaatc tgaatgagtc gatggctcgc
     9421 gaataatccg attacggcta cgcttctaat gttccccttg aatggagtcg aagaatgcgt
     9481 aatcccacgc tgttacaatg ttttcactgg tattacccgg aaggcggtaa gctctggcct
     9541 gaattggccg agcgcgccga cggttttaat gatattggta tcaatatggt ctggttgccg
     9601 cccgcctata aaggcgcatc gggcgggtat tcggtcggct acgactccta tgatttattt
     9661 gatttaggtg agtttgatca gaaaggcagc atccctacta aatatggcga taaagtacaa
     9721 ctgctggccg ccattgacgc cctgaaacgt aatgacattg cggtgctgtt ggacgtggta
     9781 gtcaaccaca aaatgggcgc ggatgaaaaa gaagctattc gcgtgcagcg cgttaatgct
     9841 gatgaccgta cgcaaattga tgaagaaatc attgaatgcg aaggctggac gcgttacacc
     9901 ttccccgctc gtgccgggca atactcacag tttatctggg atttcaaatg ttttagcggt
     9961 atcgaccata tcgaaaaccc caatgaagat ggcattttta aaattgttaa cgactacacc
    10021 ggcgaaggct ggaacgatca ggttgatgat gaactcggta atttcgatta tctgatgggc
    10081 gagaatatcg attttcgcaa tcatgccgtg acggaagaga ttaaatactg ggcgcgctgg
    10141 gtgatggaac aaacgcaatg cgacggtttt cgtcttgatg cggtcaaaca tattccagcc
    10201 tggttttata aagagtggat cgaacacgta caggaagttg cgccaaagcc gctgtttatt
    10261 gtggcggagt actggtcgca tgaagttgat aagctgcaaa cgtatattga tcaggtggaa
    10321 ggcaaaacca tgctgtttga tgcgccgctg cagatgaaat tccatgaagc atcgcgcatg
    10381 ggacgcaact acgacatgac gcagattttc actgggacgc tggtggaagc cgatcctttt
    10441 cacgccgtaa ccctcgttgc caaccacgac acccaaccgt tgcaagccct cgaagcaccg
    10501 gtcgaaccgt ggtttaaacc gctggcgtat gctctaattt tgttgcggga aaatggcgtt
    10561 ccttcggtgt tctatccgga cctctacggt gcgcattacg aagatgtcgg tggtgacggg
    10621 caaacctatc cgatagatat gccaataatc gaacagcttg atgagttaat tctcgcccgt
    10681 cagcgtttcg cccacggtgt acagacgtta tttttcgacc atccgaactg cattgccttt
    10741 agccgcagtg gcaccgacga atatcccggc tgcgtggtgg tcatgtcgaa cggagatgat
    10801 ggcgaaaaaa ccattcattt aggagagaat tacggcaata aaacctggcg tgattttctt
    10861 gggaaccggc aagagagtgt agtaaccgac gaaaacggcg aagcgacctt cttttgcaac
    10921 ggcggcagcg ttagcgtgtg ggttatcgaa gaggtgcttt aaattcatcc ccggcggcaa
    10981 gccggggaga tttcattacg gcaatggcgt cggtaacggc gctttatcca gcgcagcggc
    11041 acactctgcc gtcgggcgat ccacacgctc catcgtcata ccatcgtact caatggtgtt
    11101 accgtcacgc tcaacctcgt acagctcacg ttttacggta acgttagtga gatcatcaga
    11161 catcaatgtc aacttgcccg gcacagcaat cacccgctgc cactgacggc aatctagggt
    11221 atcgccctct ttagtgacaa tcaggctgcc tatcgcttcc gggctgacca gcgcacgttg
    11281 cggccctttg gtctgccagt agcccgccag ccagtccggc gcgggggttt tcacgacatt
    11341 gttatagttt tcgacttcgg cacagcccgc cagcaacatg agcgcgcctg caattgcgag
    11401 ttttttcatc attcgtcctg catgagaaga aaagaggatt gtggcattaa agccctgatg
    11461 tcgccagcct ttccaggttc agagagaaat ttgatctaac tattattttt gtgaattatt
    11521 accccgaaaa taaatttgca tggtgtatga ttcgcgactc ttgtttcaaa ccttctgagt
    11581 tcagaggcta cattcatgtc atggcagcaa ttcaaacacg cctggttgat taaattctgg
    11641 gcgcccatcc ccgcggtcat cgcggcgggt attctctcca cttactattt tggcatcact
    11701 ggcacctttt gggctgtcac gggtgaattt acccgttggg gcggtcagct cctgcagctg
    11761 ttcggcgtcc atgctgaaga gtggggttat tttaaaatta tccatctgga aggatcgcca
    11821 ttaacccgca tcgacgggat gatgatcctc ggtatgtttg gcggctgctt tgccgcagcg
    11881 ctgtgggcca acaatgtcaa actacgcatg ccgcgtagcc gtatccgcat tatgcaggcc
    11941 attattggcg gcattatcgc cggttttggc gcgcgtctgg caatgggctg taacctggcg
    12001 gcattcttta ccggtattcc tcagttctcg ctgcatgcct ggttctttgc catcgccact
    12061 gccattggtt catggtttgg cgcgcgcttt accctgctgc ccatcttccg tattcccgtg
    12121 aaaatgcaga aagttgctgc cgcctcaccg ctgacgcaaa aaccggatca ggcgcggcgt
    12181 cgttttcgtc tcgggatgct ggtctttttc ggcatgctgg gctgggcgct gctcacagcg
    12241 atgaaccaac ccaaactggg gctggcaatg ctgtttggcg tcggctttgg tttactgatt
    12301 gaacgtgcgc aaatctgctt tacttcggcg ttccgcgata tgtggatcac cggacgtacc
    12361 catatggcga aagcaatcat tattggtatg gcggtgagtg ccatcgggat cttcagttac
    12421 gtacagttag gcgttgaacc caaaatcatg tgggcgggac caaacgcggt aattggtggt
    12481 ttactgtttg gttttggcat cgtgctggct ggcggctgcg aaaccggctg gatgtaccgc
    12541 gcggtagaag gccaggtgca ctactggtgg gtcggtctgg gcaatgtgat cggctcaacg
    12601 attctggcgt attactggga tgatttcgct ccggcgctgg ccaccgactg ggacaaaatc
    12661 aacttgctga aaacctttgg tccgatgggg ggcctgctgg tgacatattt gctgttgttt
    12721 gctgcgctga tgttgattat cggctgggaa aaacgcttct tccgccgtgc ggcaccgcag
    12781 actgctaagg agatcgcatg aaaaatatcg ttcctgatta ccgccttgat atggtgggtg
    12841 aaccctgccc ttatccggca gtcgcaaccc ttgaggcgat gccgcagtta aaaaaagggg
    12901 aaatcctgga agtggtgagc gactgtccgc agtcgatcaa taatattcca ctggatgcgc
    12961 gtaatcacgg ctatacggtg ctggatattc agcaagacgg gccgaccatt cgttatttaa
    13021 ttcagaagta atccctcatt cccgctgtga cccacagcgg gaacatcgtt acctttgtat
    13081 cacatagatt ttacccgccc cctctcctat tcttttcagt atgatctcag gaggtttcta
    13141 tgtgtggacg ctttgcccaa tcccaaacgc gtgaagatta ccttgcgctt ctcgcggaag
    13201 atattgaacg cgatattccc tatgatcccg aacccattgg cagatacaac gtcgcgccgg
    13261 gaaccaaagt cctactgctc agtgaacgtg atgaacacct tcatctggat ccggttttct
    13321 ggggatatgc tcccggatgg tgggataaac cgccgctgat taacgcccgc gtagaaactg
    13381 cggcaaccag tcgtatgttt aaaccgctct ggcaacatgg tcgggcaatc tgttttgccg
    13441 atggctggtt tgagtggaaa aaagaaggcg acaaaaagca gccttatttt atctatcgtg
    13501 ctgatggaca acctgttttt atggcagcga taggcagcac accgttcgag cgcggtgacg
    13561 aagccgaagg atttttgatt gtcactgcag cagcagacca gggtctggta gatattcatg
    13621 accgccgccc gctggttctg tcgccagaag ctgcgcggga atggatgcgg caagagatta
    13681 gcggtaaaga agcctcagaa atcgcggcca gtggctgtgt tccagcaaac cagttctcct
    13741 ggcacccggt atcgcgcgcg gtgggtaatg ttaaaaacca gggtgcggag ttaattcaac
    13801 ctgtttgata agcctggaga ttattgatca acaatactgc gtcataagta atctctatta
    13861 gacaaagatt tcattacctg ttggcatatt gcaaaaataa caccaatacg gaatcgtcat
    13921 gttcacgatt aaaacagatg atctcaccca tccagcagtg caagcattag tggcttacca
    13981 tatttccggc atgctgcagc agtctccccc tgaaagcagt catgctttag acgtgcaaaa
    14041 attacgtaac ccgacagtga cattctggtc agtatgggaa ggcgaacaac tcgcaggaat
    14101 tggtgcgctg aagttgctgg atgataaaca tggcgaactg aaatcaatgc ggaccgcgcc
    14161 aaattattta cgtcggggtg tcgccagtct gattttacgc cacattttgc aggtcgccca
    14221 tgacagatgc cttcatcgcc tgagtttaga aacgggtaca caggctggat ttacggcctg
    14281 ccatcaactt tatttgaagc atggtttcgt tgattgcgaa ccgtttgccg attatcaact
    14341 tgatccacac agtcgatttt tgtcattgac gctatgcgaa gataatgagt tgctttgagc
    14401 cagacgcagc acattcttgc attcgacgtg ctgcgtcttt atttatcacc aacaggaaac
    14461 gccttgtcca tagacgc
//
LOCUS       DAZFAB010000109        14424 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZFAB010000109 DAZFAB010000000
VERSION     DAZFAB010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.109"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..462
                     /locus_tag="ACHJWA_003819"
     CDS             <1..462
                     /locus_tag="ACHJWA_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFO4310333.1"
                     /translation="SEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSI
                     IAEQKKESEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVVNEDDCKIRRG
                     NAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVFAGSGLS"
     gene            728..2965
                     /gene="nrfB"
                     /locus_tag="ACHJWA_003820"
     CDS             728..2965
                     /gene="nrfB"
                     /locus_tag="ACHJWA_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFO4310334.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            2952..5924
                     /gene="nfrA"
                     /locus_tag="ACHJWA_003821"
     CDS             2952..5924
                     /gene="nfrA"
                     /locus_tag="ACHJWA_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFO4310335.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSITTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAEALRLARLLYQQESANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARISQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAARQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGSNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRFFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            5925..6815
                     /gene="ybcH"
                     /locus_tag="ACHJWA_003822"
     CDS             5925..6815
                     /gene="ybcH"
                     /locus_tag="ACHJWA_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFO4310336.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAVPGVTPDGWYFSAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEHVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            6998..7759
                     /gene="envY"
                     /locus_tag="ACHJWA_003823"
     CDS             6998..7759
                     /gene="envY"
                     /locus_tag="ACHJWA_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFO4310337.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(7805..7881)
                     /locus_tag="ACHJWA_003824"
     tRNA            complement(7805..7881)
                     /locus_tag="ACHJWA_003824"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7845..7847),aa:Arg,seq:tct)
     gene            8124..8756
                     /gene="fimZ"
                     /locus_tag="ACHJWA_003825"
     CDS             8124..8756
                     /gene="fimZ"
                     /locus_tag="ACHJWA_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HFO4310338.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRY
                     LVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVELIDYAKLYELI"
     gene            complement(8759..9274)
                     /gene="sfmF"
                     /locus_tag="ACHJWA_003826"
     CDS             complement(8759..9274)
                     /gene="sfmF"
                     /locus_tag="ACHJWA_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HFO4310339.1"
                     /translation="MRRVFFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALLPFSLRLEGCPPGSVAILFTGTPASDANLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     KADAIFMINYN"
     gene            complement(9285..10292)
                     /gene="fimH"
                     /locus_tag="ACHJWA_003827"
     CDS             complement(9285..10292)
                     /gene="fimH"
                     /locus_tag="ACHJWA_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFO4310340.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNVFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAVKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            complement(10305..12914)
                     /gene="sfmD"
                     /locus_tag="ACHJWA_003828"
     CDS             complement(10305..12914)
                     /gene="sfmD"
                     /locus_tag="ACHJWA_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFO4310341.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTFVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSAHGYIPPEEWDEGIPALLLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNGSW
                     NYFRGNGYHSEQWNNIGTWVQRAIIPLKSELVMGDGNTGSDIFDGVGFRGVRLYSSDN
                     MYPDSQQGFAPTVRGIARTAAQLTIRQNGFIIYQSYVSPGAFEITDLHPTSSNGDLDV
                     TIDERDGNQQNYTIPYSTVPILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTALGGL
                     PQEFTAYGGTQLSANYTAFLLGLGRNLGNWGAVSLDVTHARSQLADDSRHEGDSIRFL
                     YAKSMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDYDYDGEHRDEPIIVNYH
                     NLRFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWVGISYSL
                     SFSWNESVGIPDNERIVGLNVSVPFNVLTKRRYTRENALDRAYASFNANRNSNGQNSW
                     LAGVGGTLLEGHNLSYHVSQGDTSNNGYTGSATANWQAAYGTLGVGYNYDRDQHDVNW
                     QLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIENQTGILTDWRGYAVMPYATVY
                     RYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGGKPVPF
                     GSLVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQA
                     VTVISAVCTHPGS"
     gene            complement(12945..13637)
                     /gene="fimC"
                     /locus_tag="ACHJWA_003829"
     CDS             complement(12945..13637)
                     /gene="fimC"
                     /locus_tag="ACHJWA_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFO4310342.1"
                     /translation="MMTKIKLLMLIIFYLIISASVHAAGGIALGATRIIYPADAKQTA
                     VWIRNSHTNERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVNSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(13857..14399)
                     /gene="fimA"
                     /locus_tag="ACHJWA_003830"
     CDS             complement(13857..14399)
                     /gene="fimA"
                     /locus_tag="ACHJWA_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO4310343.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
BASE COUNT         3817 a         3581 c         3442 g         3584 t
ORIGIN      
        1 agtgaaaaga gtcacggcag agaagaaatc cgtcttcata ttgtttgcga tgtccctgat
       61 gaacttattg atttcacgtt tgaatggaaa gggctgaaga aattatgcgt ggcagtctcc
      121 tttcggtcca taatagcaga acaaaagaaa gagtcagaaa tgacggtcag atattatatc
      181 agttctgctg atttaaccgc agagaagttc gccacagcaa tccgaaacca ctggcacgtg
      241 gagaataagc tgcactggcg tctggacgtg gtagtgaatg aagacgactg caaaataaga
      301 agaggaaatg cagcagaatt attttcaggg atacggcaca ttgctattaa tattttgacg
      361 aatgataagg tattcaaggc agggttaaga cgtaagatgc gaaaagcagc catggacaga
      421 aactatctgg cgtcagtctt tgcggggagc gggctttcgt aatcttgccc tgcctgctca
      481 cctccaggca cttccactac atcatgacct gatcggtgcc accactcctc ttcactatga
      541 cgttaacctc ccctccttga caccacgctt ttgtctacat tctacgcaaa cggcaacttt
      601 tataaccagt gtaaaaataa cgtgccgcaa tagtcatacc tattaatggt aaaaagctgt
      661 cacaattcat aaaaaacctt aatatacgcc accctaaaca taaccagcgt taatgtaagg
      721 tttttgtgtg gactggcttc ttgatgtttt tgctacctgg ctctatggct taaaagtaat
      781 cgcgataacg ttagcggtca tcatgttcat cagcgggctg gacgattttt ttattgatgt
      841 cgtctactgg gtacgccgca ttaaacgcaa gttgagtgtt tatcgccgct acccgcgaat
      901 gagttaccgc gaactgtata aaccagatga aaaaccgtta gcgattatgg ttccggcgtg
      961 gaatgaaacg ggcgtcatcg gcaatatggc cgagctggcg gcgaccacgc tcgactacga
     1021 aaactatcat atctttgttg gcacctaccc caacgacccg gacactcagc gtgatgttga
     1081 cgaagtgtgc gctcgcttcc cgaatgtgca taaggtagtc tgcgcgcgtc ctggccccac
     1141 cagcaaagcc gactgtctga acaacgtgct ggacgccatc acccagtttg agcgtagcgc
     1201 caatttcgct tttgctggtt ttattctgca tgacgccgaa gatgtgattt caccgatgga
     1261 attgcgtctg ttcaactatc tggtcgagcg taaagatctg attcagatcc cggtgtatcc
     1321 gtttgaacgc gaatggacgc acttcaccag catgacttac attgatgagt tttcagagct
     1381 gcatggcaaa gatgttccgg tgcgtgaagc cctcgccgga caagtgccca gcgcaggcgt
     1441 cggcacctgt ttcagccgcc gcgccgtgac cgctctgtta gctgacggtg acggtattgc
     1501 tttcgacgtg cagagtctga ctgaagatta cgacattggc ttccgcctga aagaaaaagg
     1561 tatgacggaa atttttgtcc gttttccggt ggtggacgaa gccaaagaac gcgagcagcg
     1621 taaattttta cagcacgcac ggacgtcaaa catgatctgc gtgcgcgaat atttccccga
     1681 taccttttcg actgcggttc gacaaaaatc tcgctggatc atcggcattg ttttccaggg
     1741 ctttaaaacc cataaatgga cctccagcct gacgctgaac tactttctct ggcgcgaccg
     1801 caaaggggca atcagtaact ttgtcagctt cctcgcaatg ctggtgatga tccagctttt
     1861 gctgttgctg gcgtatgaaa gtttgtggcc cgatgcctgg catttccttt ctatttttag
     1921 cggcagcgca tggttaatga ccctgctgtg gctaaacttt ggcttgatgg ttaaccgcat
     1981 cgtgcagcgg gtgattttcg tcactggcta ctacggcctg acgcaggggc tactatctgt
     2041 cctgcgtctt ttctggggca acctgattaa ctttatggcc aactggcgcg cgttaaaaca
     2101 ggtacttcaa cacggcgatc cacgtcgcgt cgcgtgggat aaaacaacgc atgacttccc
     2161 cagcgtcact ggcgataccc gctcgttgcg cccgttaggt caaattctgc tggaaaatca
     2221 ggtcatcact gaagaacagc tcgatacagc actgcgtaat cgcgtcgaag gtctacgcct
     2281 gggcggttca atgctgatgc aggggctgat tagcgccgag cagctggcac aggcgctggc
     2341 agagcaaaac ggcgtggcgt gggaatccat cgatgcctgg cagatcccgt cctcgctgat
     2401 tgccgaaatg ccggcctccg tggcgctgca ttatgcggta ctgccgctgc gtctggaaaa
     2461 tgatgagtta attgtcggca gtgaagatgg tattgacccg gtttcgctgg cggccctgac
     2521 gcgtaaagtc ggacgcaaag tgcgttatgt cattgttctg cggggacaaa ttgtcacggg
     2581 gttacgtcac tggtatgcac gccgacgcgg tcacgatccg cgggcaatgt tgtacaatgc
     2641 ggttcagcat cagtggctca cggaacagca ggccggtgaa atctggcggc aatatgtgcc
     2701 gcatcagttc ctgttcgccg aaatactgac cacgctcggt catattaatc gttcagcaat
     2761 taacgtgttg ttattgcgcc atgaacgcag ttctctgccg ctcggcaagt ttttggtcac
     2821 cgaaggcgtt atcagccagg aaacgttgga tcgcgtcctg acaattcaac gcgaattaca
     2881 agtttcgatg caatcactat tactcaaagc aggtttaaac acagaacagg ttgcgcaact
     2941 ggagtccgaa aatgaaggag aataacctta atcgcgtcat cggatggtct ggtttactgc
     3001 tgacgtcttt attgagtacc agcgcactcg cagacaatat cggcaccagc gcagaagagc
     3061 tggggctgag cgattatcgc cattttgtta tttatccccg tctcgataag gcgctgaagg
     3121 cacagaaaaa taacgacgaa gcaaccgcca tccgcgaatt tgaatatata caccagcagg
     3181 tgccggataa tattccgctg actttatacc ttgcggaagc ctatcgccat tttggtcatg
     3241 atgaccgggc acggctgttg cttgaggatc aactgaaacg tcacccagga gatgcccgac
     3301 ttgagcgcag tctggcggct attccggttg aagtgaaaag cattacaact gttgaagaac
     3361 tgcttgccca gcaaaaagcg tgcgatgctg cgccgaccct gcgttgtcgc agtgaagtcg
     3421 ggcagaatgc cctgcggctg gcacagttac ctgtcgccag agcgcaactg aacgacgcga
     3481 cgtttgctgc atcgccggaa ggaaaaacgc tgcgaaccga tctgctgcaa cgggcaatct
     3541 acctgaaaca atggtcccag gcagatacgc tatacaatga agcacgccag cagaacacat
     3601 taagcgcggc agaacgccgt cagtggtttg acgtgcttct tgccgggcag ctggacgatc
     3661 ggatcctggc actgcaatca caggggatct tcaccgatcc tcagtcatat attacttacg
     3721 cgaccgcgct ggcttatcgt ggcgaaaaag cacgcctcca gcattatctc attgaaaata
     3781 agccgctgtt taccacggac gcacaagaga aaagttggct ctatctgtta tctaaataca
     3841 gcgccaaccc cgttcaggca ttggcgaatt atacggtgca gtttgccgat aaccgccagt
     3901 atgttgttgg cgcgacgcta ccggtgctgt taaaagaagg tcagtacgac gcagcgcaaa
     3961 aactgctcgc caccctcccc gccaatgaaa tgcttgagga gcgttatgct gtcagcgtgg
     4021 cgacccgtaa caaggctgaa gctctgcgtc tggcacgctt gctgtatcag caagaatcgg
     4081 caaatcttac ccgcctggat caactaacct ggcaactgat gcagaacgag cagtcacggg
     4141 aagctgccga tttattgctg caacgctatc ctttccaggg cgatgcgcgt atcagccaga
     4201 ctttaatggc gcgactggcg tctctgctgg aaagtcatcc ttacctggca acgccggcga
     4261 aggtggcgat tttatcgaaa cccttaccgc tggcggagca acgtcagtgg caaagtcagt
     4321 tgccgggtat tgcagataat tgcccggcaa tagttcgctt gctgggcgat atgtcgcctt
     4381 cctacgatgc cgccgcctgg aaccgtctgg caaagtgtta tcgggacacg ctacccggtg
     4441 tggcgttgta tgcatggctt caggccgaac aacgacaacc gaacgcctgg caacatcgtg
     4501 cggtagccta tcaggcgtat caggttgagg actacgccac cgcactggcg gccaggcaga
     4561 aaatcagtct tcacgacatg agcaatgagg atctgcttgc tgctgccaat accgcccagg
     4621 cggcaggaaa tggtgcggct cgcgatcgct ggctacaaca ggcagaacaa cgtggactgg
     4681 gaagcaatgc cctctactgg tggctgcatg cgcaacgtta cattcctggt cagccggaac
     4741 tcgcactgaa cgatctcacg cgctcaatca atattgcgcc ttctgccaac gcttacgttg
     4801 cgcgggcgac aatttatcgc caacgtcata atgtcccggc cgcggtgagt gatttgcgcg
     4861 ccgcgctgga actggaaccg aataatagca acacccaggc agcgcttggt tacgccttgt
     4921 gggatagcgg tgatatcgca cagtcgcggg aaatgctcga acaggcgcat aaagggctac
     4981 cggacgatcc ggcactgatc cgacaactgg cctatgtgaa ccagcgtctg gatgacatgc
     5041 ctgcgacgca gcactacgcc cggctggtga ttgatgacat tgataatcag gcgctgataa
     5101 ccccactgac cccagagcaa aatcagcaac gcttcaattt ccgccgtctg catgaggagg
     5161 tcggtcgccg ctggacgttc agtttcgatt cttccatcgg cttgcgttcc ggggcaatga
     5221 gtaccgctaa caataatgtt ggcggcgcag cgccagggaa aagctatcgt agctacgggc
     5281 aactggaagc cgaataccgc atcggacgca atatgctgct ggaaggcgac ctgctctcag
     5341 tttatagccg cttctttgcc gataccggag aaaacggggt gatgatgccg gtgaaaaatc
     5401 cgatgtccgg caccggtctg cgctggaagc cgctgcgcga tcagatcttt ttcctcgccg
     5461 tcgaacagca gttgccgctg aacgggcaaa atggcgcatc cgataccatg ctgcgcgcca
     5521 gcgcctcatt ctttaatggc ggcaaataca gcgacgaatg gcacccgaac ggttcaggct
     5581 ggtttgccca aaacctgtac ctcgatgcgg cgcaatatat ccgccaggat attcaggcgt
     5641 ggacggcaga ttatcgcgtc agctggcatc agaaggtagc taacggacag actattgagc
     5701 cttacgctca cgttcaggac aacggctatc gtgataaagg cactcagggc gcgcagcttg
     5761 gcggtgtcgg ggtccgctgg aatatctgga ccggcgagac gcactacgac gcctggccgc
     5821 acaaagtcag tctcggcgtc gagtatcaac atacctttaa ggcgattaat caacgtaacg
     5881 gagagcgcaa caacgcgttt ctcaccattg gagtgcactg gtaaatgcgt aagttcattt
     5941 tcgtattgct gacactgctt ttggtcagcc ctttttcctt tgcgatgaaa ggtattatct
     6001 ggcaaccaca aaaccgtgat agtcaggtta ccgataccca gtggcagggg ctgatgagtc
     6061 agttacgttt gcaaggcttc gataccctgg ttttgcaatg gacccgttac ggcgatgcat
     6121 tcacccagcc agaacagcgc gcgttattgt ttaagcgggc cgcagctgcg caacaggctg
     6181 gcctgaagct gattgtcggg ctgaacgccg atccggaatt ttttatgcac cagaaacagt
     6241 catccgcggc gctggaaagc tatcttaatc gcctgctggc tgccgatctc cagcaagcca
     6301 gattatggag tgccgtacct ggcgtaacgc cggatggctg gtacttcagc gctgaaattg
     6361 acgacctgaa ctggcgcagc gaagccgccc gtcagccttt gctaacatgg ttaaacaacg
     6421 cgcagcggct gattagcgat gtttcagcaa aaccggttta tatcagtagt tttttcgccg
     6481 gaaacatgtc gccagatggc tatcgccaac tgctggaaca cgttaaagca accggcgtta
     6541 atgtctgggt acaggatggc agcggcgtgg ataaacttac cgctgaacag cgtgaacgtt
     6601 atctacaggc cagcgccgat tgccaaagct ccgccccagc aagcggcatt gtttatgaac
     6661 tgtttgtcgc cggcaaaggc aaaaccttta cagcgaaacc gaaaccggac gcagaaattg
     6721 cctcgctgtt agcgaaacgt tcctcttgcg gtaaagacac tctctatttc tctctgcgct
     6781 atttgcccgt cgcgcacggc attctcgagt attaaatctc ctccaggtaa gtcgggtacg
     6841 acctggctta cctctttcgc tctcgtcaga attccatcaa gattaattgc taaaaagcgt
     6901 gctaaagaaa gaagatcgtt atcggcgaac gggccgatca ctcatgcagg aagcatagat
     6961 cctttgagcg gagtttctga tatctgagga gtgcgaaatg caattgagca gcagtgaacc
     7021 ttgcgtggtg atcctgaccg aaaaagaggt agaggtaagc gtcaataacc atgctacgtt
     7081 tacccttccg aaaaactacc tggccgcctt cgcgtgcaac aataacgtca ttgaactctc
     7141 aacgttaaat cacgtattaa tcacccacat caaccgtaac atcatcaacg attatctgtt
     7201 gtttttaaat aagaacttaa cctgtgtaaa gccctggtcg cggctggcta ccccggttat
     7261 cgcttgtcat agccgtacac cggaagtgtt ccggctagcc gccaaccaca gcaagcagca
     7321 acccagcaaa ccctgcgaag cggagttgac gcgcgcattg ctttttaccg tattgtctaa
     7381 ctttcttgag caatcgcggt ttattgccct actgatgtat atcttacgca gcagcgtccg
     7441 cgacagcgtc tgccgcatta ttcaaagcga tattcagcat tactggaatc tgcgaattgt
     7501 cgccagttcg ctatgtttaa gccccagcct gctcaaaaag aaattaaaaa acgaaaatac
     7561 cagctatagc cagattgtca cagagtgtcg tatgcgttac gccgtacaga tgttattgat
     7621 ggataacaaa aatatcactc aggtggcgca attatgtggc tatagcagca cgtcgtactt
     7681 tatctctgtt tttaaggcgt tttacggcct gacaccgttg aattatctcg ccaaacagcg
     7741 acaaaaagtg atgtggtgaa gggcaaagcg gaaacggata agacgggcat aaataaagaa
     7801 gaaatggcgc gccctgcagg attcgaacct gcggcccacg acttagaagg tcgttgctct
     7861 atccaactga gctaagggcg cgttgatacc gcaatgcggt gtaatcgcgt gaattatacg
     7921 gtcaaccctt gctgagtcaa tggcttttga tctggttgct gaacaagtga acgaccgcgt
     7981 ctgattttct gatttatttc gctatagcgg caaacaaacg cacaccgctg cgcgtctgaa
     8041 tcaagaaaac ccgtattttc atgtatcaaa gtacaattta ccgccctaac ggaaaattgt
     8101 ccgctcctat gagactggta actatgaaac caacgtcggt gatcattatg gatactcatc
     8161 ctatcatcag aatgtctatt gaagttctgt tgcaaaaaaa cagtgaattg cagattgtcc
     8221 tgaaaacgga tgattaccgc ataaccatcg attatctccg aacccgtcct gttgatttaa
     8281 tcattatgga tatagacttg cccggaacag acggttttac cttcctgaaa aggatcaaac
     8341 aaatccagag cacagtgaaa gtgttatttt tatcatcgaa atcagaatgc ttttatgctg
     8401 gcagagcgat acaagctggt gctaacggtt ttgtcagtaa atgcaatgat cagaatgata
     8461 tttttcatgc cgttcagatg atcctctccg gatacacgtt ttttcccagc gaaacgctta
     8521 actatataaa aagcaataaa tgtagtacga atagttcaac ggtcactgtg ctatctaatc
     8581 gtgaagtgac catattacgt tatctagtta gcggattatc taataaagaa attgccgata
     8641 agttattact tagcaataaa acagttagtg cgcataaatc taatatttat ggcaagctag
     8701 gtttgcattc aattgtagag cttatcgact acgccaaatt atacgaatta atataatatt
     8761 aattataatt gatcataaat atcgcatccg ctttcgccac acctggccga acaccgctgg
     8821 ctaacgctcg ataattggca aaaaagttta gtgtgacatt gccatttgca tctacttcct
     8881 cagtcgggct cgcctccccc agtgcgagcc gggagcgatc gctattacgt aattcgatgg
     8941 cgacggtttg tgccattgca ggatcatcca gagccagcag gttggcatca gatgccggcg
     9001 ttcccgtaaa taaaatcgca actgaacccg gaggacatcc ctccagccgc aggctaaaag
     9061 ggaggagtgc cgtggtatcg ccagcgttca gtaattgtgt cgtaggccat ctgcctaaat
     9121 ctaccgtctt atcaatatcc gctgtgttta cggtacagga gaaatcaaca acgttaccgt
     9181 gcaaattgat attaatcgtt cctaaagggt cagctgccca tccactggaa ctccacagta
     9241 acccgcagaa acagctaaag aatactcttc tcattctccg tacctcattg ataatcgacg
     9301 cgtaaatacc ccagcgcgct aaacggccct tcggtcggtt tttgaccggt aatactgata
     9361 ggccaggcgc gaagtgtgac attggctgcc gcagctgcat ccagacggaa aggaataacg
     9421 ctattgagat cgttaggcgt gatcggcgta tcgttctgat cggcgacaat aaaacctaaa
     9481 tcctgattgt ccgacaccat cgcctgacca gaaacggcac tggcttccag acgcattgtt
     9541 aaataagcct gcgcagcaac attcgtacat ttgaccgcaa tgctcttggt ttgcggcatg
     9601 acaccagcag gccgattacc cggccctgcc gcactaaata acgatgcgcc gatatcacca
     9661 aaatcaaatt caacaatctg cccggcattt aattcgcagt tttgcggtac ttcaacccgg
     9721 ccaccaaaac taatggtata aacaggtgta accaacggat cgtaaggcgt tgatgtcaca
     9781 tacacggtaa acatcacctg tctggggatc tccaccatgt taataaaggg ccgaataacc
     9841 tttaaacgaa atatcaaacg agaatccatc accccgaacg gaaggttttg cgaaacgtta
     9901 ggatctgttc ccatacgaat ataattttgc gggggaaatt gaatatccat cacactatca
     9961 accagactca tcgcacctaa tagataatca tttaattgca tatatttata attatcgata
    10021 gtttcctgaa caataaagtt agtgacataa ctacggtatg tataattcac cagcgtgccg
    10081 ggtggacaaa ttgctgagac acctacccag ccggattttt ccggtaaaac aacaatctgc
    10141 cctggctggt tattggtgct attaaagaca tttgagagat catagtgttc atcttctgct
    10201 acgccagtac tatttgcaca aacagtagcc caggatatgt ttgccagaca caaacatgcc
    10261 atcgccaata ataatctgca gattatcttc attgataatt tcctttatga gccaggatgt
    10321 gtgcaaactg ccgaaataac agtgacggct tgctgtaagc tttgcttcgg caatacatag
    10381 tgcgcaatgc agcgtgagtt cgcgccgtct ccccactgaa ccagtaattc accagacaat
    10441 ggcgcaccac ttaaataaac ttgcccgtca tcacccacca tactggttat tccggtactg
    10501 ttttcccgta ccagtgatcc aaacggcacc ggttttccgc cctgggtaac ggtaatgagc
    10561 gcccgcacgc ctatgcgggt atcaaaattg gcacgaacca acgcgccttg cgtcggcaca
    10621 acgctgctaa tatttttttc aacatcgatg gaattcccca tcgtattggt atcaagcgcg
    10681 atacggttat accgataaac cgtggcatac ggcatcaccg catagccgcg ccagtcggtt
    10741 aaaatgccag tttgattttc aatgcgtaca ccgcctgcgc caggcgcttt aatcaaaaca
    10801 ttggtatccc ctaaaggctg gctcagcgtt atgccatttt catgtccgac cacaccgcca
    10861 gacagctgcc agttaacgtc atgttgatcg cggtcgtagt tatacccgac ccccagcgta
    10921 ccgtaagcgg cctgccagtt tgccgtggcg ctgcccgtat acccattatt cgaggtatca
    10981 ccctggctta cgtgataact caggttgtgg ccttccagta aggtcccacc tacacctgcc
    11041 agccagctat tttgcccgtt gctgttacgg ttggcgttaa aggaggcata agcgcggtcg
    11101 agcgcatttt cccgggtgta gcgacgtttg gtcagaacat tgaaaggcac tgaaacatta
    11161 agtccgacaa tacgttcgtt atcggggatc cctacagatt cattccacga aaatgagagt
    11221 gaataactga tgccaaccca gctgctggta taccccacct gataccaggt atctgaatcc
    11281 gaagtattcc agtatttttg atgggtacca gagatataaa gcgagccaaa gtcattaagt
    11341 gattgtgaaa tatttaactg caaacggtct ttacggctaa agcgtaaatt gtggtaattc
    11401 acgattatcg gttcatcccg atgctctccg tcataatcgt aatcatattc gtacccctcc
    11461 attcgacgat acgcaacatc atcaagggta taaaaacctt gtgtcgaata gcggtaaccc
    11521 attaactgaa aattggtgcc gaaggtgttc atcgatttcg catagaggaa gcgaatggaa
    11581 tccccctcat gacgactgtc gtcggctaac tgactgcgcg catgggtcac atccagcgac
    11641 actgcgcccc agttcccgag gttgcgcccc agccctaata aaaaggcggt gtaattggca
    11701 gataattgcg tcccgccgta ggcagtaaat tcctgtggta aaccgccgag tgccgtgccc
    11761 tgaaaaaaga aaggcgatga ttgctgacta ttaccgctac gaaaatcgcc cgccgtcagg
    11821 tcaaatttga aacgcccttc gcgttgtaaa attggcactg ttgaatacgg aatagtgtaa
    11881 ttctgctgat tgccatcgcg ttcgtcgatg gtgacgtcca gatcgccatt tgaagatgtc
    11941 gggtgcaaat ctgtaatttc aaaagcgccg ggggaaacat agctttgata gataataaaa
    12001 ccattttgcc gaatcgttag ctgggccgcc gtacgggcaa tcccacgtac cgttggggca
    12061 aacccttgct ggctatcagg atacatatta tcggaagaat aaagccgtac accacgaaat
    12121 ccaacaccat cgaaaatatc acttcctgta ttgccgtctc ccattaccag ttcacttttc
    12181 agcggaataa tggcgcgctg tacccaggtg ccaatattat tccactgttc tgaatgatat
    12241 ccatttccgc gaaaatagtt ccaggaacca ttgttgcgta aacgccacgg gccaatatta
    12301 atcccgctga gctcactaaa aaaatagcta tcgttaccat tacctctgtt accggtgaaa
    12361 ttataattca ggagtaaagc aggaatacct tcatcccact cttcaggcgg aatgtaaccg
    12421 tgagcgctac tcaacaacgc tatctgagga atagtgatgt tcaggcgcat cgctgcaaaa
    12481 tcaaagttaa ttgtggcatc aggtacagct ttcagtaaat tgatgcattt attgttttgc
    12541 tgctgggcta actcgggaaa tgcactgcta ttaaggccaa ttcgttcaag gagtacctgg
    12601 ttaaaacagg gcattaaccc acctgattta tcacctgtat tttctgttgt cgattcaaat
    12661 acgatatcct gtgaaccaat gaactcatca ttacgccaga gatccacccg atacacgccc
    12721 gcaggttgat gattacctct ctcaaagcgc gataaatcag caacgaatgt gccattctct
    12781 aataaaaatg ccgggttgaa ataactttcc gcatagctta aaaaggaggg taaaatggca
    12841 agagatgaat aacaaattcc ggccagacac caggtatacc tctgcggaat atccgtagta
    12901 gtgggtattt tcataattag ctctgatatt gtcagatgat tcggttagtt taagttcact
    12961 tctctgaccg gggttactga accataatca ttaacggtct gaaatttcag ctttccctgc
    13021 actcctgagg gtaagggaat ttgtgaatta actctgggtg cagccatagc attccccaac
    13081 ttttggctgc ccacttgtaa gttaaccagg gtgacataaa aaggtgatgg attatgaaca
    13141 ttgatatagt taccggatcg cgaaaacttg agtgtatccg gcgcttctgc gggtaattct
    13201 tgtagttgaa ttggacggag aaataacttc atgcgcgata aaatcgccag ttgcaaaaca
    13261 ttcctgccgt tcaatgcatt tttatctacc gaaggaatcg tcttaacatt catccagaac
    13321 agagactcac gatctgctgc cagcggtgga ccggtgtaaa taatacgcaa agtattttcg
    13381 cttttgggtt cactaacaaa tagtggcggt gtaatgatga atgacttttc ttttacaccg
    13441 ctgctgtttt caatccacga attgaccaga aaacgctcat tggtatggct atttctaatc
    13501 cataccgcag tctgtttagc atcagcggga taaataatac gcgtggcacc taatgcgatc
    13561 cctccggcag catggacgct ggccgaaatg attaaataaa atataatgag catcaataac
    13621 tttattttag tcatcatagt taatacgcct tgtgtattcc tgacggcaaa aataagaaat
    13681 ataaagaaat gattgctttg gtttcttaca acacatcatt cccttttcat ttaataaagc
    13741 ggcatgatac aaagtgaaca tgatttcgat ttacattgcc accataccgc ttcagacaac
    13801 cggtacatta ctttgttata agacgtgata tataattcaa aacaacgtgg ttttgattat
    13861 tcgtatctca taataaaagt cgcgtcagca tttgcttgcc ctgctgatgc acttgtaccg
    13921 gtgcctttat aacgtgcaga aaaatgaaga acgttggtcc cggggatcaa gttctggttg
    13981 gttgagaagc tattcccatc aggtttgaga attgcggatg tattatcaag tatttcaata
    14041 ccgacacccg ttgctgttgt tgtattggta ctggatgcaa tggccaataa attatcattg
    14101 attgcatcag cctgaccaga aaatgcaacg gcagcattag cggcaacaac aggatcgcag
    14161 tcgttcaact gaatggtgaa tggaattaat gcagaggtat taccaacagc attgaaaata
    14221 tcggtacgat attgaccgag tgtgacaacc tggtctgccg agtcagtatt cactgaacag
    14281 gcagcattca ccagttcacc ttcaaagtga attgtaccgc catctacaac ggcagcataa
    14341 ctacccgtag cggcgaatag tgcggcagcc agcgcagacg aaataaatct taatttcata
    14401 tatattcctt caatctcatt tatc
//
LOCUS       DAZFAB010000110        14251 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZFAB010000110 DAZFAB010000000
VERSION     DAZFAB010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14251
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.110"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            207..1265
                     /gene="citC"
                     /locus_tag="ACHJWA_003831"
     CDS             207..1265
                     /gene="citC"
                     /locus_tag="ACHJWA_003831"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFO4310344.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIAMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1280..1576
                     /gene="citD"
                     /locus_tag="ACHJWA_003832"
     CDS             1280..1576
                     /gene="citD"
                     /locus_tag="ACHJWA_003832"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFO4310345.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1573..2481
                     /gene="citE"
                     /locus_tag="ACHJWA_003833"
     CDS             1573..2481
                     /gene="citE"
                     /locus_tag="ACHJWA_003833"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFO4310346.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2492..4024
                     /gene="citF"
                     /locus_tag="ACHJWA_003834"
     CDS             2492..4024
                     /gene="citF"
                     /locus_tag="ACHJWA_003834"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFO4310347.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLILASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTLFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4028..4579
                     /gene="citX"
                     /locus_tag="ACHJWA_003835"
     CDS             4028..4579
                     /gene="citX"
                     /locus_tag="ACHJWA_003835"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFO4310348.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4554..5432
                     /gene="citG"
                     /locus_tag="ACHJWA_003836"
     CDS             4554..5432
                     /gene="citG"
                     /locus_tag="ACHJWA_003836"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFO4310349.1"
                     /translation="MSMPATLTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQLLTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5483..6946
                     /gene="citT"
                     /locus_tag="ACHJWA_003837"
     CDS             5483..6946
                     /gene="citT"
                     /locus_tag="ACHJWA_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFO4310350.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            7060..7866
                     /gene="rna"
                     /locus_tag="ACHJWA_003838"
     CDS             7060..7866
                     /gene="rna"
                     /locus_tag="ACHJWA_003838"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFO4310351.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQVSIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8096..8506
                     /gene="rnk"
                     /locus_tag="ACHJWA_003839"
     CDS             8096..8506
                     /gene="rnk"
                     /locus_tag="ACHJWA_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFO4310352.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8644..8733
                     /gene="yldA"
                     /locus_tag="ACHJWA_003840"
     CDS             8644..8733
                     /gene="yldA"
                     /locus_tag="ACHJWA_003840"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFO4310353.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(8737..9975)
                     /gene="ybdR"
                     /locus_tag="ACHJWA_003841"
     CDS             complement(8737..9975)
                     /gene="ybdR"
                     /locus_tag="ACHJWA_003841"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HFO4310354.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRLQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10196..10624
                     /gene="uspG"
                     /locus_tag="ACHJWA_003842"
     CDS             10196..10624
                     /gene="uspG"
                     /locus_tag="ACHJWA_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFO4310355.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10745..12310)
                     /gene="ahpF"
                     /locus_tag="ACHJWA_003843"
     CDS             complement(10745..12310)
                     /gene="ahpF"
                     /locus_tag="ACHJWA_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFO4310356.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12439..13002)
                     /gene="ahpC"
                     /locus_tag="ACHJWA_003844"
     CDS             complement(12439..13002)
                     /gene="ahpC"
                     /locus_tag="ACHJWA_003844"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFO4310357.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13374..14120
                     /gene="dsbG"
                     /locus_tag="ACHJWA_003845"
     CDS             13374..14120
                     /gene="dsbG"
                     /locus_tag="ACHJWA_003845"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308670.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFO4310358.1"
                     /translation="MLKKILLLALLPAIAFAEKLPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQEYEASGGKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
BASE COUNT         3409 a         3755 c         3565 g         3522 t
ORIGIN      
        1 aataaataaa aaaccactaa ctctatgtga aataaatcaa aatttcacgc cgaaatactc
       61 cttaggatgt atagcgaaag gagaaaaaga tatacctcga tcacccccct ttctcccaag
      121 tgaaaataaa aggttatcag tttgcaacat tgaacaacat tcgttgcaaa tcgataacaa
      181 catgcacctt caggatacta tttattatgt tcggcaatga tattttcacc cgcgtaaaac
      241 gttcagaaaa taaaaaaatg gcggaaatcg cccaattcct gcatgaaaat gatttgagcg
      301 ttgacaccac agtcgaagta tttattaccg taacccgcga tgaaaagctt atcgcgtgcg
      361 gtggaattgc cggaaatatt attaaatgcg ttgctatcag tgaatccgtt cgcggtgaag
      421 gactggcgct gacattagcc actgaactga taaacctcgc ctatgagcgg cacagcacgc
      481 atctgtttat ttataccaaa accgaatacg aggcgctgtt ccgccagtgc ggtttttcca
      541 cgctgaccag cgtacccggc gtgatggtgc tgatggaaaa cagcgccacg cgactgaaac
      601 gctatgccga atcgctgaaa aaatttcgtc atccagggaa caagattggc tgcattgcga
      661 tgaacgccaa tccctttacg aatggtcacc gttatctgat tcaacaagct gcggcacagt
      721 gcgactggtt gcatctgttt ttagtcaaag aagattcttc acgcttcccc tatgaagacc
      781 ggctggatct ggtgttaaaa ggcaccgccg atattccacg cctgactgtg catcgcggct
      841 ccgaatacat catctcccgc gctacgttcc cttgctactt cattaaagaa cagagcgtca
      901 ttaaccattg ttacaccgaa attgatctga agattttccg tcagtacctc gctcccgcgc
      961 tgggtgtaac tcaccgcttt gtcggtactg aacccttttg tcgcgttacc gcccagtaca
     1021 accaggatat gcgctactgg ctggaaacgc cgactatctc cgcagcgccc atcgaactgg
     1081 ttgaaattga gcggctgcgt taccaggaga tgccgatatc cgcttcccgg gtacgtcaac
     1141 tgctggcgaa aaacgatctc acggctatcg cgccgctggt ccctgcagtc acgctgcatt
     1201 atttgcagaa cctgcttgag cactcccgcc aggacgcggc agctcgtcaa aagacccccg
     1261 catgagaaac aggtgaaaaa tgaaaataaa ccagcccgcc gttgcaggca cccttgagtc
     1321 tggggatgtg atgatacgca tcgccccact cgatacgcag gatatcgacc tgcaaatcaa
     1381 tagcagcgtt gagaaacagt ttggcgatgc aattcgcacc accattctgg acgttctcgc
     1441 ccgctacaac gtgcgcggcg tacagctgaa tgtcgatgat aaaggcgcac tggactgcat
     1501 tttacgtgca cgactggaag ccctgctggc acgcgccagc ggtatcccgg ctctgccatg
     1561 ggaggattgc caatgatttc cgcttcgctg caacaacgta aaactcgcac ccgccgcagc
     1621 atgttgtttg tgcctggtgc caatgccgcg atggtcagca actccttcat ctacccggct
     1681 gatgccctga tgtttgacct cgaagactcc gtggcattac gtgaaaaaga caccgcccgc
     1741 cgcatggttt atcacgcgct gcaacatccg ctgtatcgcg atattgaaac cattgtgcgt
     1801 gtaaacgcgc tggattccga atggggtgtt aacgacctgg aagccgtcgt tcgcggtggt
     1861 gcggacgttg tacgtctgcc gaaaaccgat accgctcagg atgttctgga tatcgaaaaa
     1921 gagatcctgc gtatagaaaa agcctgtggt cgtgaacccg gcagcaccgg cctgctggcg
     1981 gcgattgaat ctccgctggg cattacccgc gcagtggaaa tcgctcacgc ttccgagcgt
     2041 ttgatcggta tcgccctcgg tgcagaagac tatgtgcgca acctgcgtac agaacgctcc
     2101 ccggaaggaa ctgaactgct gttcgcacgc tgttccattt tgcaggccgc gcgctctgcg
     2161 ggtattcagg cgttcgatac cgtctattcc gacgctaaca acgaagccgg attcctgcaa
     2221 gaagccgccc acatcaaaca gctgggcttt gacggcaaat cgctgatcaa cccgcgtcag
     2281 attgatctgc tgcacaacct ctacgcaccg acccagaaag aagtggatca cgcccgccgc
     2341 gtcgtagaag ccgctgaagc cgccgctcgc gaaggcctcg gcgtggtttc cctgaacggc
     2401 aagatggtgg acggtccggt tatcgatcgt gcccgtctgg tgctctcccg tgcagaactt
     2461 tccggcatcc gcgaagaata aggcaatcaa aatgacgcag aaaattgaac aatctcaacg
     2521 acaagaacgg gtagcggcct ggaatcgtcg cgctgaatgc gatcttgccg ctttccagaa
     2581 ctcaccaaag caaacctacc aggctgaaaa agcgcgcgat cgcaaactgt gcgccaacct
     2641 ggaagaagcg attcgtcgct ctggtttaca ggacggcatg acggtttcct tccatcacgc
     2701 tttccgtggc ggtgacctga ccgtcaatat ggtgatggac gtcatcgcga agatgggctt
     2761 taaaaacctg atcctggcgt ccagctccct gagtgattgc catgcgccgc tggtagaaca
     2821 cattcgccag ggcgtggtta cccgcattta tacctccggc ctgcgtggtc cactggcgga
     2881 agagatctcc cgtggtctgc tggcggaacc ggtgcaaatc cactctcacg gcggacgtgt
     2941 gcatctggta cagagcggcg aactgaatat cgacgtggct ttcctcggcg tcccgtcctg
     3001 tgatgaattc ggtaatgcca acggctacac cggtaaagcc tgctgcggct ccctcggcta
     3061 tgcaatggtt gatgccgaca acgcaaaaca ggttgtgatg cttaccgaag aactgctgcc
     3121 ttatccgcat aatccggcaa gcattgagca agatcaggtt gatttaatcg tcaaagttga
     3181 tcgcgttggc gatgctgcaa aaatcggcgc tggtgctacc cgtatgacca ctaacccgcg
     3241 cgaactgctt attgcccgta gcgctgcgga tgtgattgtc aactcaggct acttcaaaga
     3301 aggtttctcc atgcaaaccg gcaccggcgg cgcatcgctg gcggtaaccc ttttcctgga
     3361 agacaaaatg cgtagccgcg atattcgcgc cgacttcgcc ctcggtggta ttaccgcgac
     3421 gatggttgat ctgcacgaaa aaggtctgat ccgcaaactg ctggatgtac agagctttga
     3481 cagccacgct gcgcaatcgc tggcccgtaa ccccaatcac atcgaaatca gcgccaacca
     3541 gtacgctaac tggggttcga aaggtgcatc ggttgatcgt ctcgacgtgg tggtactgag
     3601 cgcgctggaa attgacaccc agttcaacgt taacgtgctg accggctctg acggcgtact
     3661 gcgtggtgct tccggtggtc actgcgatac cgcgattgcc tctgcgcttt ccatcatcgt
     3721 cgcgccgctg gtacgcggtc gtattccgac tctggtggat aacgtgctga cctgcatcac
     3781 cccgggctcc agtgtcgata ttctggtcac agaccacggt atcgcagtta acccggcacg
     3841 tccggaactg gcagaacgtc tgcaggaagc gggcattaaa gtggtttcca ttgagtggct
     3901 gcgcgaacgt gcgcgtctgc tgaccggtga accacagccg attgaattca ccgaccgcgt
     3961 cgttgccgtt gtgcgttacc gcgatggctc ggtgatcgat gttgtgcatc aggtgaagga
     4021 ataagccatg cacctgcttc ctgaactcgc cagccaccat gcggtgtcaa ttcccgagct
     4081 gctcgtcagc cgggatgaaa ggcaagcacg gcaacacgtc tggctcaagc gccatcctgt
     4141 tccactggtc tcctttaccg tggttgcgcc tgggccgatt aaagacagtg aggtcacacg
     4201 ccgaattttt aatcatggcg tgacagcctt gcgtgcctta gccgcaaaac agggctggca
     4261 aattcaggag caggctgcac tggtttccgc cagcgggccg gagggtatgt tgagcattgc
     4321 cgccccggct cgcgacctca agctcgccac cattgagctt gaacatagtc atcctctcgg
     4381 gcggttatgg gatatcgatg tcctgacgcc cgaaggcgaa attctctccc gccgcgacta
     4441 ttcactgccg cctcgccgct gcctgttgtg cgagcaaagc gcagccgtct gcgcgcgtgg
     4501 aaaaacccat caactgaccg atttactcaa ccgcatggag gcactgctga acgatgtcga
     4561 tgcctgcaac gttaactaaa accacaaagc ttgcgacgtc attaatcgat gagtacgccc
     4621 tgctgggctg gcgcgccatg ctgactgaag tcaatctgtc accgaaacca ggtctcgtgg
     4681 atcgcattaa ctgcggtgcg cataaagata tggcgctgga agatttccac cgcagcgcgc
     4741 tggcgattca gggctggcta ccccgtttca ttgaatttgg tgcctgtagt gcggaaatgg
     4801 caccagaagc ggtactccac ggattacgcc caattggtat ggcttgcgaa ggtgatatgt
     4861 tccgcgccac cgcgggcgta aacacgcata agggcagcat tttttcttta gggctactgt
     4921 gtgcggcaat tggccgtttg cttcagctca accagttgct aacgccaaca accgtttgtt
     4981 ctacggcggc aagtttctgc cgtggcctga ccgatcgcga actgcgtacc aataattcac
     5041 aactgacggc aggtcaacgg ttgtaccagc agcttggcct taccggcgca cgcggtgaag
     5101 ccgaagcggg ttatccactg gtgatcaacc acgccttgcc gcattacctc actctgctgg
     5161 atcaggggtt agatcctgaa ctggcattgc tcgatacctt gctcctactg atggcgatca
     5221 acggcgatac caacgttgct tcgcgcggtg gcgagggggg cctgcgctgg ctacagcgcg
     5281 aggcgcaaac attattgcaa aaagggggca ttcgaacccc cgcagatctc gattatctcc
     5341 ggcagttcga cagggagtgt atcgaacgaa atctcagtcc aggcggcagt gccgacctgc
     5401 tgatccttac ctggttttta gcacagattt aattatttaa gaacttgata aatttggaaa
     5461 tattaatttt cggagaaccc gtatgtcttt agcaaaagat aatatatgga aactattggc
     5521 cccactggtg gtgatgggtg tcatgtttct tatccctgtc cccgacggta tgccgccgca
     5581 ggcatggcat tacttcgctg tgtttgtggc aatgattgtc ggcatgatcc tcgagccaat
     5641 tccggcaaca gcgatcagtt ttattgcggt tactatttgc gttattggca gtaattacct
     5701 gctctttgat gccaaagaat tagctgaccc agcgtttaat gcgcaaaaac aggcgctgaa
     5761 atggggcctg gctggttttt ccagcactac ggtatggctg gtatttggcg catttatttt
     5821 tgcattaggg tatgaagttt ccgggttagg tcgtcgcatt gcccttttcc tggtgaaatt
     5881 catgggcaaa cgcacgctga cgttgggtta tgcgattgtc attatcgaca ttctgctggc
     5941 accgtttaca ccgtccaaca ccgcgcgtac cgggggtacg gtttttccgg tcattaaaaa
     6001 cctgccgccg ttgtttaaat cattcccgaa cgatccgtcc gcgcgtcgta ttggcggcta
     6061 tttgatgtgg atgatggtca ttagtaccag tctgagttcg tccatgtttg tcaccggtgc
     6121 ggcaccaaac gtgctgggtc tggagttcgt cagcaaaatt gccggtatcc agattagctg
     6181 gttgcagtgg ttcctctgct tcctgccggt tggggttatc ttgcttatca ttgcgccgtg
     6241 gctttcctac gtgctgtaca aaccggaaat cacacacagt gaagaagtgg caacctgggc
     6301 gggtgatgaa ctaaaaacca tgggtgcgct gacacgcaga gagtggacgc tgattggcct
     6361 tgtattgctc agcttaggtt tgtgggtatt tggcagtgaa gtcattaatg ctactgcggt
     6421 tggtctgctg gcagtttcgc taatgctggc tctgcacgtt gtgccgtgga aagacattac
     6481 ccgctataac agcgcatgga acacgctggt caacctggca actctggttg tgatggctaa
     6541 cggcctgact cgttctggtt ttattgactg gttcgccggt accatgagta cgcacctgga
     6601 aggattctca ccaaacgcaa cggtgattgt actggttctg gtgttctact ttgcacacta
     6661 cctgtttgcc agcctgtctg cgcacaccgc aaccatgctg ccggttattc tggccgtcgg
     6721 taaaggtatt ccgggcgtac caatggaaca actgtgtatc ctgctggtgc tgtctatcgg
     6781 tatcatgggc tgtctgacgc cgtatgcaac cggtcctggg gtgattattt acggctgtgg
     6841 ctatgtgaaa tcaaaagatt actggcgtct tggcgcaatc ttcggggtga tttacatctc
     6901 tatgttgctg ttggttggct ggccgattct cgccatgtgg aactaatacg ttcgccaaac
     6961 gctgaataaa tcatcacgcc cgccaggtta gcccacctgg cgggcttttt tatgatttaa
     7021 tagatagtat tcagtgtatg tatgagttcc acacccatta tgaaagcatt ctggcgtaac
     7081 gccgcgttgc tcgcggtttc tctgcttccc ttctcttctg ccaacgccgt agcgttgcag
     7141 gcaaaacagt atggcgattt tgatcgctat gtcctggccc tctcctggca aaccggattt
     7201 tgccagagtc aacacgatcg aaatcgtaac gaacgagatg aatgtcgcct gcaaaccgaa
     7261 acgaccaaca aagctgattt tctgaccgta catggtctgt ggccaggatt gcctaaatcg
     7321 gttgctgccc gtggtgttga tgaacgccgc tggatgcgct tcggttgcgc tactcgccca
     7381 atcccgaatc taccagaagc gcgcgccagc cggatgtgtt catcgccgga aaccggatta
     7441 tcactggaaa cggccgctaa actaagtgaa gtcatgccag gagctggcgg acgttcctgc
     7501 ctggaacgct acgaatatgc caaacacggt gcctgctttg gttttgatcc ggacgcatac
     7561 ttcggtacga tggtacgcct gaatcaagag ataaaagaga gcgaagccgg aaaattcctt
     7621 gcggataatt acggtaaaac agtgagccgc cgtgactttg acgccgcctt tgccaaaagc
     7681 tggggaaaag agaacgtgaa agcagttaag ctaacgtgcc agggtaaccc tgcgtatttg
     7741 actgaaattc aggtctcgat caaagctgac gccatcaacg ctccgctttc tgcaaactca
     7801 ttcttgccac aaccacaccc aggtaactgt ggcaaaacct ttgtgattga taaagcgggt
     7861 tattaatcct ctgatttaca aaacacgcta attcgtcttt cataaaccat gacgattaag
     7921 aattatacat aatgcgctgc attgaggaat tccctgaatg tgacgcaaat cacttcaaag
     7981 agtgagcaac agtcagtatc atcatgtgag ttaaaccctc gccgcctgac ggtgagggtt
     8041 ttcttttggg tatgtttatc tgcgacactc gcagtaccga cgacatggag taaaaatgtc
     8101 cagaccaact atcatcatta acgacctgga tgccgaacgc atcgatattc tgctggagca
     8161 acccgcctat gctggtttgc caatcgccga cgcgttaaac gcagagttgg atcgcgccca
     8221 aatgtgttcg ccagaagaga tgccacacga cgtggtgaca atgaacagcc gggttaaatt
     8281 ccgcaatctt agcgatggcg aagtgcgtgt gcgcacgctg gtgtatccgg caaaaatgac
     8341 cgatagcaat actcagcttt ccgttatggc tccggtaggt gccgcactgc tgggactgcg
     8401 cgttggcgat tccattcact gggaacttcc gggcggcgtt gcaacccacc ttgaagtgct
     8461 ggaactcgaa taccagccag aagctgctgg cgactacctg ctttaatccc atctgtacaa
     8521 gcgataactc gctttgccca gaggatgcac tcgcatcctc tttatgcaaa atcagcaatt
     8581 tttcctggtt ctcactgttc acttttgctg caaaattatt ccgtttcgtt tgtgggaata
     8641 accatggcag aagcatttta catcctgata ggatttctga ttatggcggc gattatcgtc
     8701 atggctgtcc tttacctcga aaaccactcc tgacgctcat attgttcccc ccggcatcgc
     8761 attcaccaga cctgaaaccg ccttctgcgc cgcctctgcg ctttgtgcac cgggtaccag
     8821 aatcacctta cggcactcct cttcacgctt ttcaaagatc tcatatcccc gggcggcctc
     8881 ttcaaacggc atatagtggg taacaatttc ttctggtttc agtaatcctt tctcaattaa
     8941 cggtaataat tctcccagcc atgcgtgaac gtgggtctgt cccattttaa acgtcaaccc
     9001 tttatcaaag gcgtcgccaa acaggaaacc gtgaataaat ccagcgtaga cgcccggtac
     9061 gctaacaatg ccgccacgcc tgaccgccgc aatacactga cgcaacgctt taccgctgct
     9121 gccctccagt ttcaggttag tcagcaccgt ttccgtggtg ctgcctttcg cttcaaaacc
     9181 gacggcgtct attactgcat ccacgccccg gtgacctgcc gtttgttcaa taattgactg
     9241 tgccggatcg ctatcttcat cgaaattaat cgggatcgcg ccgtagcggt cagcggcgaa
     9301 acgcaagcgg tagggatgat gatcaacaac aaaaatctgt tccgcaccga gcaaccgtgc
     9361 acaggcgatt gtcaacaatc ccacaggacc ggcaccatag acagcaacgc ttgaaccttg
     9421 ttggatctgc gcattttttg ctgcctgcca tgccgttggc agaatatcag aaaggaaaag
     9481 ggctttatca tctgaaagca aaggcggtac tttaaacggc cccacattcc ctttagggac
     9541 acggacatac tccgcctgtc caccaggaac gccaccatac aggtgactat aaccaaacaa
     9601 tgccgcggga gctggtatct gttttttatt gagcgcagcg cctttacccg cattggtatt
     9661 ttcgcaggcg gcatattgct gcagtcgaca gaaaaaacag tcgccacaag caatgacgaa
     9721 cggaattacc acccggtcgc ctttttgcaa attttttaca tcctttccgg tttcaactac
     9781 ttcccccata aattcatgac caaaaatatc gccatgttta acctggggta ttttgcctcg
     9841 ataaagatgg aggtcagagc cacagatcgc cgttgccgtt atacgcagaa taatatcatc
     9901 tgcctgttca atgcccggat cgggaacatt ttctacctga acgtgatgtg ggccgtgata
     9961 agtcaatgct ttcatgcaac ctccagagac agttcaaaaa agagtcagtc tttaagggta
    10021 gctatcacag cgggaaaacg ggcggtaccg caggttttgg gagaattcct ggtgttcagt
    10081 gagaatatat tcatcacaga aacagttcgt aaggaaaaag aaccagacaa atatcaggga
    10141 taaatattat aatcatggcg aatgatttgt tcatgattaa cagggagaaa gggttatgta
    10201 taagacaatc attatgccag ttgatgtatt tgaaatggaa ttgagcgaca aagctgttcg
    10261 ccacgctgaa ttcctcgccc aggatgacgg agttattcat ctacttcacg tactacccgg
    10321 gtcagccagc ctgagcctgc accgttttgc cgctgatgtg cgtcgttttg aagagcatct
    10381 gcaacatgaa gcagaagaac gtttgcaaac gatggtcagc cacttcacca tcgatccttc
    10441 ccgcattaaa caacatgtcc gttttggtag cgtgcgggat gaagtcaatg agttagcaga
    10501 agaactgggg gctgatgttg tagttattgg ttctcgcaac ccatcgattt cgacccatct
    10561 gttaggttct aacgcctcga gcgtgatccg ccacgccaat ctgcctgtac tggtcgttcg
    10621 ctaacagata aaagtattta tgccgccctg cctggaacat cccggcaggg ctttttaatg
    10681 ccataaaaaa gccgccaggt ttgaccccgg cggctaagca attgcaggtg aatcttactt
    10741 cttcttatgc agttttggtg cgaatcaggt agtcaaaagc actcagagag gctttggcac
    10801 cttcgccagt ggcgatgatg atctgcttgt acggaaccgt cgtacagtca cccgctgcga
    10861 acacgccttt cacgttggtt tcgcatttcg catcaatgat aatctcgccc atgcggttac
    10921 gttcgactgc gccttcgagc cagttggtgt tcggcagcag accaatctgc acgaaaatac
    10981 cggccagttc gatgttgtga atatcgccgc tgacacgatc tcgatattcc agaccaacga
    11041 ctttgctgcc gtcgcctttc acttccgtgg tttgcgcatt cagaataatg tcgacgtttt
    11101 tcaggctgcg cagtttgtcc tgcagaacct ggtcggcttt catttctggc gcaaattcca
    11161 gcagcgttac gtgctcaacg atacccgcca ggtcaattgc cgcttccacg ccggagttac
    11221 cgccgccgat aaccgctacg cgtttacctt taaacagcgg gccgtcgcag tgcgggcagt
    11281 aggtcacgcc tttggtgcga tactgatctt cgcccggaac gttcatgttg cgccattttg
    11341 caccggtcgc cacgataatg ctgcgtgctt tcagtaccgc gccagaagct gtttcaatct
    11401 gatgcagacc accttcaact gctgctggga tcagtttgct ggcgctctgg ctgtcgatca
    11461 catcaacgtc gtattcatca acgtgaactt tcagtgcgcc tgccagcttc tgcccttcag
    11521 tcttcggtac agaaatgtag ttttcgatat caacggtatc gaggatctga ccaccaaaac
    11581 gttcgcccat cagaccggta cggatgcctt tacgtgcgga gtaaattgct gccgctgcac
    11641 ccgccgggcc ggaaccgacg attaatacgt cataagcatc acgcttgttc agctcttctg
    11701 ccgcacgttt ttccgcgccc gtatcaattt tggcaacgat ttcagtcaaa gtcatgcggc
    11761 cctgaccaaa ctctttcccg ttcacgaaca ctgccggaac gcccatcacg ttacgatcag
    11821 tgatctcgtt ctggaaggtg ccgccgtcaa ttgcagtgtg cttgatgcgc gggttcaata
    11881 cgctcatcag gttcagcgcc tgtaccacgt ccgggcagtt atggcaagag agcgagtaat
    11941 aggtttcgaa ttcaaaatca ccgtcaatat ggcgaatctg ctccagcaga gactgcgctt
    12001 ctttcgacgg atgaccaccg gtccacagta acgccagtac cagcgaggtg aactcgtggc
    12061 ccagcgggga gcctgcaaaa cgtggcccct ggttagaacc tgggttggtg atcaggaaag
    12121 acggcttacg caccggcaag ctgttatctt ctttaaaggt gactttgtct gacagttctg
    12181 cgatttcagc caacagttcc ttgatttctg ccgatttagc gctgtcatcc agcgtggcaa
    12241 ttaactcaac aggcttggtc aatttctcaa ggtaagcctt gagttgagtt ttcatatttg
    12301 tgtcgagcat gtttatctcc tgggctttaa acatcatcat gcaagcggcc tctgcggcta
    12361 cctggatgca gcttgcatca tggtgcgggg aagaaaatcg ggtgccgcag cacccgatta
    12421 attacgcgat tgcggaactt agattttacc aaccaggtcc agagacggag ccagagttgc
    12481 ttcaccttct ttccatttag ccgggcaaac ttcacctggg tgagaagcta cgtactgtgc
    12541 tgctttgatt ttacgcagca ggtcagacgc gtcacggcca atgccttcag cggtaacttc
    12601 gattgcctgg atgataccct gcgggtcaac aacgaaggtc gcacggtcag ccagaccttc
    12661 atcttcacgc atgttgtcga agttacgggt cagggcgcca gtcgggtcgc cgatcatcgc
    12721 atatttgatt ttagcgatgg tttcagagct gctgtgccat gctttgtggg tgaagtgagt
    12781 atcggtagat actgcgtata cgtctacgcc cagtttctgc agttcttcgt agtggtcagc
    12841 aacgtcaccc agttcggtcg ggcatacgaa agtaaagtca gccgggtaga agaagaagac
    12901 gctccagcgg ccttcggtat ctttttcggt gatttcgatg aattcgccgt ttttgaatgc
    12961 ctggttttta aaaggtttaa ttttggtgtt aatcaaggac atctatactt cctccgtgtt
    13021 ttcgatgaga tgtaaggtaa ccaattttta ccgattcggc taatgcgttt ccattatcaa
    13081 atcgataagt tttaccttac aaccttcgta agacaacttt gtgaactttg caccgccagc
    13141 ggcgataaaa agtaaaatgg ccccctttcc aggtgaccgt gtaatctgaa ctaccctttg
    13201 gtatcttcag cgccagtctg caggctgcgt tgttgctctt acaaatgcaa caacgacatg
    13261 gattacaaca ccctcataaa caaagggcaa tcacctgatc taagctctta cctatgacaa
    13321 tgataggtta tgccttttac tcgacttttg cactgactga aaaggacaaa ttaatgttaa
    13381 aaaagatact tttactggct ctgcttcctg caatcgcctt cgcagagaaa cttcctgctc
    13441 cagtaaaagc gattgaaaaa cagggcatta caatcatcaa aacgttcgat gcccccggag
    13501 gaatgaaagg ttatctcgga aagtatcagg atatgggcgt caccatctac ctgactccag
    13561 atggtaagca cgctatctct ggttacatgt acaacgagaa aggtgaaaac ctgagtaaca
    13621 cacttatcga aaaagaaatt tacgcaccag ccggacgcga aatgtggcaa cggatggaac
    13681 aatcccactg gctcctcgac ggtaaaaaag atgcgccggt cattgtctac gtcttcgccg
    13741 atccgttctg cccatattgt aaacagttct ggcagcaggc gcgcccgtgg gtagattctg
    13801 gcaaagtaca gttgagaaca ttgttggttg gggttatcaa accagaaagt ccggcgacag
    13861 ccgcggcaat tcttgcctcc aaagatcccg caaaaacctg gcaagaatat gaagcctctg
    13921 gtggcaagct taagctaaac gtgcctgcaa acgtaagtac agagcaaatg aaagtgttaa
    13981 gcgacaacga gaaactcatg gacgatctgg gggcaaatgt cactccggct atctattaca
    14041 tgagtaagga aaatacgctg caacaggccg tggggttgcc cgatcagaaa acgcttaata
    14101 tcattatggg aaataaataa aaggtaattc atggatggtt gaactatatc cctgcttttc
    14161 aaccatccta aaaaagcatg aattgctcgc tctgccctaa gtggctgtgc tattttagaa
    14221 aaacttaatc ctgagttaag caaaaaaaac a
//
LOCUS       DAZFAB010000111        14189 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZFAB010000111 DAZFAB010000000
VERSION     DAZFAB010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14189
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.111"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            517..2148
                     /gene="oppA"
                     /locus_tag="ACHJWA_003846"
     CDS             517..2148
                     /gene="oppA"
                     /locus_tag="ACHJWA_003846"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFO4310359.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2234..3154
                     /gene="oppB"
                     /locus_tag="ACHJWA_003847"
     CDS             2234..3154
                     /gene="oppB"
                     /locus_tag="ACHJWA_003847"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFO4310360.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3169..4077
                     /gene="oppC"
                     /locus_tag="ACHJWA_003848"
     CDS             3169..4077
                     /gene="oppC"
                     /locus_tag="ACHJWA_003848"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFO4310361.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            4089..5102
                     /gene="oppD"
                     /locus_tag="ACHJWA_003849"
     CDS             4089..5102
                     /gene="oppD"
                     /locus_tag="ACHJWA_003849"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFO4310362.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            5099..6103
                     /gene="oppF"
                     /locus_tag="ACHJWA_003850"
     CDS             5099..6103
                     /gene="oppF"
                     /locus_tag="ACHJWA_003850"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFO4310363.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6156..6485)
                     /gene="yciU"
                     /locus_tag="ACHJWA_003851"
     CDS             complement(6156..6485)
                     /gene="yciU"
                     /locus_tag="ACHJWA_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFO4310364.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6520..7980)
                     /gene="cls"
                     /locus_tag="ACHJWA_003852"
     CDS             complement(6520..7980)
                     /gene="cls"
                     /locus_tag="ACHJWA_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFO4310365.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFHSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKVYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            8123..8296
                     /gene="yciY"
                     /locus_tag="ACHJWA_003853"
     CDS             8123..8296
                     /gene="yciY"
                     /locus_tag="ACHJWA_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFO4310366.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8351..9604)
                     /gene="kch"
                     /locus_tag="ACHJWA_003854"
     CDS             complement(8351..9604)
                     /gene="kch"
                     /locus_tag="ACHJWA_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFO4310367.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(9905..10201)
                     /gene="yciI"
                     /locus_tag="ACHJWA_003855"
     CDS             complement(9905..10201)
                     /gene="yciI"
                     /locus_tag="ACHJWA_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFO4310368.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            10425..11144
                     /gene="tonB"
                     /locus_tag="ACHJWA_003856"
     CDS             10425..11144
                     /gene="tonB"
                     /locus_tag="ACHJWA_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFO4310369.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQQKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            complement(11184..11582)
                     /gene="yciA"
                     /locus_tag="ACHJWA_003857"
     CDS             complement(11184..11582)
                     /gene="yciA"
                     /locus_tag="ACHJWA_003857"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFO4310370.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATDALFKYVAVDPEGKPRALPVE"
     gene            complement(11687..12226)
                     /gene="yciB"
                     /locus_tag="ACHJWA_003858"
     CDS             complement(11687..12226)
                     /gene="yciB"
                     /locus_tag="ACHJWA_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFO4310371.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(12256..12999)
                     /gene="yciC"
                     /locus_tag="ACHJWA_003859"
     CDS             complement(12256..12999)
                     /gene="yciC"
                     /locus_tag="ACHJWA_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFO4310372.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGVFASIRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(13027..13116)
                     /locus_tag="ACHJWA_003860"
                     /pseudo
     CDS             complement(13027..13116)
                     /locus_tag="ACHJWA_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            13356..13994
                     /gene="ompW"
                     /locus_tag="ACHJWA_003861"
     CDS             13356..13994
                     /gene="ompW"
                     /locus_tag="ACHJWA_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFO4310373.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            complement(14040..>14189)
                     /locus_tag="ACHJWA_003862"
     CDS             complement(14040..>14189)
                     /locus_tag="ACHJWA_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFO4310374.1"
                     /translation="PSFHEQRSLSERLFREQGVDTKILLGHSNQKMTDIYNDARGKEW
                     KKLVI"
BASE COUNT         3807 a         3341 c         3482 g         3559 t
ORIGIN      
        1 attccactgc ctttggtaga ggaaagtgct aaataataat caattgttaa attattgtgc
       61 atttcactac tggaactgta atcagaaaag atcgacatgc ttaaccaatc tctatttgat
      121 tgaatttaaa gatgtttgtt aagacatgga tgcaagctat agattctgat acggtcaata
      181 aaagagaatt gcttaacaat tttgcaaaat gtattggcga gtaagaaccg catttggtac
      241 tttccgggca accgtcaggc gattctttat tggtaatgag aataattaac aattaaagag
      301 cgtcgcgaaa gaataatgtg tctcgacagg ggagacacag tacgaatcga cataaggtga
      361 tcgtctgaat caccagaata aataaagtcg gtgatagcaa tccgtaacga taaagtaacc
      421 tgacagcaga aagtctccga gcctgtgcag ggtcccaatc cgggattaca catgctggtt
      481 aataccagta attataatga gggagtccaa aaaacaatga ccaacatcac caagagaagt
      541 ttagtagcag ctggcgttct ggctgcgcta atggcaggga atgtcgcgct ggcagctgat
      601 gtacccgcag gcgtcacact ggcggaaaaa caaacgctgg tacgtaacaa tggttcagaa
      661 gttcagtcat tagatccgca caaaattgaa ggtgttccgg agtctaatat cagccgagac
      721 ctgtttgaag gcttactggt cagcgatctt gacggccatc cggcacctgg cgtcgctgaa
      781 tcctgggata ataaagacgc gaaagtctgg accttccatt tgcgtaaaga tgcgaaatgg
      841 tctgatggca cgccagtcac agcacaagac tttgtgtata gctggcaacg ttctgttgat
      901 ccgaacaccg cttctccgta tgccagttat ctgcaatatg ggcatatcgc cggtattgat
      961 gaaattcttg aagggaaaaa accgattacc gatctcggcg tgaaagctat tgatgatcac
     1021 acattagaag tcaccttaag tgaacccgtt ccgtacttct ataaattact tgttcaccca
     1081 tcaacttcac cggtgccaaa agccgctatc gagaaattcg gcgaaaaatg gacccagcct
     1141 ggtaatatcg tcaccaacgg tgcctatacc ttaaaagatt gggtcgtaaa cgaacgaatc
     1201 gttcttgaac gcagcccaac ctactggaac aacgcgaaaa ccgttattaa ccaggtaacc
     1261 tatttgccca ttgcttctga agttaccgat gtcaaccgct accgtagtgg tgaaatcgac
     1321 atgacttata acaacatgcc gatcgaattg ttccagaagc tgaaaaaaga gatcccggac
     1381 gaagttcacg ttgatccata cctgtgcact tattattacg aaattaacaa ccagaaaccg
     1441 ccattcaacg atgtgcgtgt gcgtaccgca ctgaaactgg gtatggaccg cgatatcatt
     1501 gttaataaag tgaaagcgca gggcgacatg cccgcctatg gttacactcc accgtatact
     1561 gatggcgcaa aattgactca gccggaatgg tttggctgga gccaggaaaa acgtaacgaa
     1621 gaagcgaaaa aactgctggc tgaagcgggt tataccgcag acaaaccgtt gaccatcaac
     1681 ctgttgtata acacctccga tctgcataaa aagctggcga ttgctgcctc ttcattgtgg
     1741 aagaaaaaca ttggtgtaaa cgtcaaactg gttaaccagg agtggaaaac gttcctcgac
     1801 acccgtcacc agggtacttt tgatgtggcc cgtgcaggct ggtgtgctga ctacaacgaa
     1861 ccaacttcct tcctgaacac catgctgtcg aacagctcga tgaataccgc gcattataag
     1921 agcccggcct ttgacagcat tatggcggaa acgctgaaag tgactgacga ggcgcagcgc
     1981 acagctctgt acactaaagc agaacaacag ctggataagg attcggccat tgttcctgtt
     2041 tattactacg tgaatgcgcg tctggtgaaa ccgtgggttg gtggttatac cggcaaagat
     2101 ccgctggata atacctacac ccggaatatg tacattgtga agcactgatg gcaatacgtg
     2161 gggcaggagt gtcctgctcc acggtgtctg atttttatcg cattacagaa ggcacaggcc
     2221 agaaggtagg gcaatgttaa aatttattct acgtcgctgt ctggaagcga ttccgacgct
     2281 atttattctt attactattt cgttctttat gatgcgcctc gcgccgggaa gcccttttac
     2341 cggcgaacgt actttaccgc cggaagtaat ggccaatatc gaagcgaaat atcatcttaa
     2401 tgatccgatc atgacacagt atttcagcta cctgaaacaa ctggcgcatg gcgatttcgg
     2461 tccatcgttt aaatataaag attattcggt caatgacctg gttgcatcca gttttcccgt
     2521 ttctgccaaa ctgggagccg cagcattttt ccttgcggta atactgggtg ttagtgctgg
     2581 cgttattgcc gcattaaaac aaaacaccaa atgggactat accgtgatgg ggctggcaat
     2641 gaccggggtt gttatcccca gttttgtggt tgcgccatta ttagtcatga tatttgcgat
     2701 cattttgcat tggctgccgg gcggtggctg gaatggtggg gcgcttaaat tcatgatatt
     2761 gccgatggtg gcgttgtcac tcgcctatat cgccagtatt gcgcgtatta cccgtggctc
     2821 tatgattgaa gtattacact ccaactttat tcgtactgcc cgggcgaaag ggttacctat
     2881 gcggcggatc attttacgcc acgcattaaa acctgctctg ttacccgtgc tctcctatat
     2941 gggccccgca tttgtcggca ttattaccgg ttctatggtt atcgaaacca tttatggttt
     3001 gccggggatt gggcaactgt tcgttaatgg cgcattgaac cgtgactatt ccttagtgtt
     3061 aagcctgacc atcctggttg gtgcgttaac cattttgttt aatgccattg tcgatgtgct
     3121 atatgcggtt atcgacccga aaatccgtta ctgatactgg agctcgcgat gatgttaagt
     3181 aagaaaaaca gcgagacgct ggaaaatttc agtgaaaagc tggaggtcga agggcgcagc
     3241 ttgtggcagg acgcacgtcg acgttttatg cataaccgtg cggcggttgc aagtctgata
     3301 gtgctggtgc tgatcgcgtt atttgtaatc ctggcaccga tgctttcgca gtttgcctat
     3361 gacgatactg actgggcgat gatgtccagc gccccggata tggagtccgg tcactacttt
     3421 ggtactgact catccggtcg cgacctgctt gtgcgcgttg cgattggcgg acgtatctca
     3481 ctcatggtcg gtgttgctgc ggcactggtg gcagtggtcg tggggacact ttatggttcg
     3541 ctttccggtt atctgggcgg taaagtggat tcggtaatga tgcgtttgct ggaaatcctc
     3601 aactccttcc cattcatgtt cttcgtcatt ttgctggtga cctttttcgg tcaaaacatc
     3661 ctgctgattt tcgtggcgat aggcatggtt tcctggctgg atatggctcg tattgtgcgt
     3721 gggcaaaccc tgagtctgaa gcgcaaagag tttattgagg cggcacaagt tggcggtgta
     3781 tcgacgtcgg gcattgttat tcgccacatt gtgccgaacg tactcggtgt ggtggtggtc
     3841 tacgcatcgc tactggtgcc cagcatgatc ctctttgaat ctttccttag cttcctgggg
     3901 ttgggtacgc aagagccgtt aagcagctgg ggggcattgc tgagtgatgg cgcgaactca
     3961 atggaagtct ctccatggtt actgttgttc ccagcgggat tcctcgtggt gacgctgttt
     4021 tgtttcaact ttatcggcga tggcttgcgt gatgccctcg acccgaaaga tcgttaagga
     4081 gtgcagccat gagcgtaatt gaaactgcaa ctgtgccgct cgcacaacaa caggctgacg
     4141 cactgctgaa cgtgaaagat ttgcgtgtca cctttagtac cccggacggc gacgtcacgg
     4201 cggtcaatga tttaaatttt tccctacgtg ccggagaaac gctgggcatt gtaggtgagt
     4261 ctggttcggg taaatcgcaa actgcatttg cgttgatggg cctgttggct gccaacggac
     4321 gcattggcgg atcggcaacc ttcaatgggc gtgaaatcct caatttgcca gagcgtgaac
     4381 tcaataaact gcgcgctgaa caaatctcaa tgatttttca ggacccaatg acttcgttga
     4441 atccctatat gcgcgtcggt gagcagttga tggaagtgct gatgctgcat aagaacatga
     4501 gcaaagctga ggcgtttgaa gagtcggtgc ggatgctcga tgcggtaaaa atgccggaag
     4561 cacgtaaacg catgaaaatg tacccgcacg aattttctgg cggcatgcgt cagcgagtta
     4621 tgattgcgat ggcattgcta tgtcgaccta agctgctgat tgcggatgaa cccactactg
     4681 cgctggacgt caccgtacag gcgcagatca tgacgctatt gaatgaactg aagcgggaat
     4741 ttaataccgc catcattatg atcacccacg atcttggcgt ggtggcgggg atctgcgaca
     4801 aagtgctggt aatgtacgcc gggcgcacga tggaatatgg caacgcgcgc gatgtctttt
     4861 atcaacccgt tcatccttat tctatcggtt tgctcaacgc ggtgcctcgt ctcgatgcgg
     4921 aaggtgaaac aatgttgact attccgggta atccgccaaa cctgctgcga ttaccgaaag
     4981 gttgcccatt ccagccacgt tgtccgcatg cgatggaaat ttgtagtagc gcaccgccgc
     5041 tggaagagtt tacgcctggc cgtctgcgtg cttgctttaa accggtggag gaactgttat
     5101 gaatgctgta accgaaggaa gaaaagtcct cctcgaaatc gccgatctga aagtgcactt
     5161 tgaaatcaaa gatggcaaac agtggttctg gcaaccgccg aaaacgctca aagccgtaga
     5221 tggtgtaact cttcgcctgt atgaagggga aacattaggt gtggtagggg aatcgggatg
     5281 cggtaagtcc acctttgctc gcgccatcat tggcctggtt aaagcgaccg acggtcatgt
     5341 tgcctggtta ggtaaagagt tgctgggcat gaaacccgat gaatggcgtg ccgttcgcag
     5401 tgatattcag atgattttcc aggatccgtt ggcatcgcta aacccgcgta tgaccatcgg
     5461 cgagatcatc gctgaaccac tgcgtactta tcatccgaaa atgtcacgcc aggaagttcg
     5521 cgagcgcgtg aaggcgatga tgctgaaagt cgggttattg cctaacctga ttaaccgcta
     5581 tccgcatgag ttctctggtg ggcagtgcca gcgtatcggg attgcgcgtg ctcttattct
     5641 tgaaccgaag ctgattatct gtgatgagcc ggtgtcggcg ctggacgtgt caattcaggc
     5701 acaggtggtc aacctgctcc agcaattgca acgtgagatg ggattgtcat taatttttat
     5761 cgctcatgac ctggccgtgg taaaacacat ttccgatcgt gtgttggtga tgtatctcgg
     5821 ccatgcggta gaactgggga cctatgatga ggtctaccac aatccactac atccttacac
     5881 cagggcattg atgtcggcag tccccatacc tgatccggat ctggagaaga acaaaaccat
     5941 ccagttactg gaaggggaat taccgtcgcc gatcaacccg ccttccggtt gtgttttccg
     6001 tacccgttgc ccgattgccg gtccggagtg tgccaaaaca cgtcctgtgc tggaggggag
     6061 tttcagacac gccgtttctt gcctgaaagt cgatccgctt taaacacaaa aagggctgac
     6121 agctgtcagc cctttttcac gccgaaagtg attatttatt cccgccagat gatatggcaa
     6181 agtttgtgat ctttttcgcg gcataacaga atgcgggcga aaacatcgtt gatttcacca
     6241 tcttcactgt ccgccagacc aatcacaact tcggcaaaaa agtctgggtt caaatcgaaa
     6301 tcaacatgtt cctgccagtc ttccgccgga tcgaataact ccgcgccgcc gcgctcttca
     6361 aactgaagat tgaacagcag aacatcagct ggatcgagat tgtcggcagc cagttcgaga
     6421 aaaatatcgt aggcctgctc aagcgtttca tcttcagtca ggcgattgtt tagatccata
     6481 tccatagtca ctacctgttt aacctctgtt ggcgacgttt tacagcaacg ggctgaagaa
     6541 gtaaaacagt cgctcggcga cacgttgcca taatggacgt tttagccata aacgggcatc
     6601 gagcagacgt gaacgcgaaa tataatcgtc ctgaacggcg gcaaggtctg caccaaaacc
     6661 tttatcgtcg attgccaggg taatctcgaa attcagccac agactacgca tatcaaggtt
     6721 aacggtgcca accagactta gttcgccatc gaccagcacg ctcttggtat gcagtaaccc
     6781 gccttcaaac tgataaactt taaccccagc agcaagcagt tccgtaaaga atgcgcgact
     6841 ggcccagccg accagcatcg agtcattttt tcgcggaagg ataatactga catccacccc
     6901 gcgctgcgcc gccgtgcaaa tcgcatgaag taaatcatcg cttggcacaa agtagggcgt
     6961 ggtcatgatc aaatattcac gcgccgaata agccgcagtc aataatgcct ggtgaatgag
     7021 atcttccgga aagccggggc cagaagcaat tgtgtgaatg gtatgaccgc tggcctgctc
     7081 aaacggcata atattgacat caggtggtgg cggtagaata cgtttccctg tttcaatctc
     7141 ccagtcgcag gaataaataa tccccatcgc ggtggcgatg gggccttcca tacgcgccat
     7201 cagatcaatc cattgcccta cgcccgcatc ttgtttgaag tagcgagggt cgaccatatt
     7261 catgctgccg gtgtacgcga tgtaattatc gatcatgatc atcttgcgat gttggcgcag
     7321 gtccatacgg cgtaaaaaca cacgcatcag attgaccttt aaggcttcga ccacttcaat
     7381 accggcatta cgcattagct cgggccacgg gctgtggaaa aaagctacac tcccggcgga
     7441 gtcgagcatc aatcggcaat gaatgccgcg tcgcgcagcc gccattaatg attcagccac
     7501 ctggtccgcc atgccgccgg gctgccagat ataaaacacc atctcaatat tatggcgcgc
     7561 gagctggatg tcgcggatta acgcctgcat cacatcatct gactcggtca tcagttgtag
     7621 ctgattccct ttgaccccag cgatcccctg acgacgctcg caaagcttga ataatggcgc
     7681 agcgacactg ctattttctt cggcaaagat atgcttacag gctttaaggt cgttaagcca
     7741 ttttgcggtg gaaggccaca tcgctctggc gcgctcagcg cggcgtttgc ctaaatggag
     7801 ctcgccaacg gcaagatagg caataattcc gactaacggc agaatgtaaa taatcaacag
     7861 ccaggccatc gcggagggaa ctgcgcgtcg tttcattaga atgcgtaaag ttacgcctgc
     7921 aatgagcaac cagtatccca gaatggccaa ccaactcacc aacgtataaa cggttgtcat
     7981 agattagaaa tccttttgaa agcgcattgt tatgagttta cgcatatgga ttcatctggc
     8041 aaataaaaac gcgggaaaag cactggtcag cgtcgggggc gggggtataa tgaccattct
     8101 gttattgcat agagtagtta acatgaagcg gagtagaacg gaagtggggc gctggcgcat
     8161 gcagcgtcag gctagccgac gtaaatcgcg ttggcttgag gggcagtcgc gccgaaatat
     8221 gcgtatccac agcatcagga agtgcattct aaacaaacag cgtaactcgt tattgtttgc
     8281 gatctacaat atctaaatgt actgggcacc attgcggtgc ccaataagtt tactcttccg
     8341 tctttaaact ctatttttgc gccgattctt tactgtcagc tttcgtttct tgttcatcgt
     8401 tatcgctgaa aataccatga cctgagttca acaacattga aacgagcata tcattattaa
     8461 tctcttcacc atttaaaact cgcgccagaa tttcgctgcc aaacagttgc ggtgagagaa
     8521 tgatatccgg atgtaccatc ttaatcttat tcaggttttt gctatcactg acggcgagaa
     8581 ctgttttgac atcactgctc atatcttttg ccgagagtac aacaaacgcg ttatctgcat
     8641 cgttatcact cagcgccaga atggcccggc aacgatcgat tcccgctttc tttaatacag
     8701 aactgtcatt actgtcaccg gggataacat cagcgttatc gcctaagcgt tgctcaagtt
     8761 gcttgatatc atcttcaggc aagttgctga taaccgttac gttttgtccg cgttgattca
     8821 gttgcaggat cgtattaatg gcgagaatcg aatgtccgca aacaataaaa tgatctttac
     8881 gatgcattgt atgattgttt ccttttacaa gtttgttgaa tcccccgcgg ataagcggac
     8941 caaaaattga ggtcatagat gtggcaaaaa cggtaatgcc ggaaataatg accgaaatag
     9001 tgaacaatcg tgctgattca gaaacaggga caatatcgcc atagccgacg gttgacatgg
     9061 tttctatcga aaaatagaac gcggtcatca aactttctat tcgcggatta aaaccttcgc
     9121 ttaaataaag cgcaccgtag gttgagtaaa acagtaatgt cgtgaaacta ataaatgcaa
     9181 aaattgtccc ggctgcggcg ctactgtggg agaagtcttt gcgcagtatc agtaaaaaca
     9241 ccagcgtaaa aatgcaaaat ccaatactaa atttcagcca gggataaaaa tgcagggtgt
     9301 aaattagcgc tatcaacaac aaaatgatac tgattgccca tgcgagcttt gcccggaaca
     9361 gtaagccaat ggagttcagg acaagaaaga cgccgagcat aaatagtggg gcgtttgcca
     9421 gcaaggagag atccaactct gaaaatgctt tgatatggaa aatatcaagc aaattgaccg
     9481 acataccata gattgtttta aaaataagca acccatttaa aaagacggcc agcgcgagaa
     9541 tgtcgtgccg tagggtcacc cataaatttg ttgctgtttg tttgaatgta gcccagtgac
     9601 tcactgatac ctctgtaacc acaattttgt ttaattatct taacgtgcac attttctgaa
     9661 catacatgcg acacgtgaat gtgttaaaca atagtagatg actttatccg cagtaatcaa
     9721 aggttttgag tgagcaactc tgcgtcagga aagggcattt ttcctgaaca tgctcacatc
     9781 atataaagaa atacagataa agttattatc tgcttgtggt ggtgaatgca atgaccgacc
     9841 tataaggaaa ggccaaacaa gaacacggtt gcaaaaaccg tgcccttaaa tattggatct
     9901 ctactcagaa cactttctta aatggtttca ctgaaacgtg ttcatagact cctgccgcta
     9961 cgtacgggtc agcatcggcc caggcctgag ctgcttccag cgattcaaat tcagcaataa
    10021 cggttgagcc agtaaatccc gcagcccctg gatcgttact gtctaccgct ggcattggac
    10081 cagctgtcaa caaacgacct tcatcatgca gtaactgtaa acgtgctaaa tgtgccggac
    10141 gaacggaaag gcgtttttcg aggctatcag ctttgtcttg agcgtaaata acatacaaca
    10201 cgggcacaac tccttgttcg ggaaagttgt aaagtacgtt atttgaaagg gcgaagatct
    10261 gcaacggaaa gatgatgtct ttgttaagac catgcataaa gtaaggtcaa ttacgccaaa
    10321 aatgacattt tcactgatcc tgatcgtctt gccttattga atatgattgc tatttgcatt
    10381 taaaatcgag acctggtttt tctactgaaa tgattatgac ttcaatgacc cttgatttac
    10441 ctcgccgctt cccctggccg acgttacttt cggtctgcat tcatggtgct gttgtggcgg
    10501 gtctgctcta tacctcggta catcaggtta ttgaactacc tgcgcctgcg cagccgattt
    10561 ctgtcacgat ggttgcgcct gctgatctcg aaccgccaca agccgttcag ccgccaccgg
    10621 agccggtggt agagccagaa ccggaacctg agccgatccc cgaaccgcca aaagaagcac
    10681 cggtggtcat tgaaaagccg aagccgaaac ctaagccaaa accgaagccg gtgaaaaagg
    10741 tacaggagca gcaaaaacgc gatgtcaaac ccgtagagtc gcgtccggca tcaccgtttg
    10801 aaaatacggc accggcacgc ccgacatcaa gtacagcaac ggctgcaacc agcaagccgg
    10861 ttaccagtgt ggcttcagga ccacgcgcat taagccgtaa tcagccgcag tatccggcac
    10921 gagcacaggc attgcgcatt gaagggcagg ttaaagttaa atttgacgtc acgccggatg
    10981 gtcgcgtgga taacgtacaa atcctctcag ccaagcctgc gaacatgttt gagcgtgagg
    11041 tgaaaaatgc gatgcgcaga tggcgttatg agccgggtaa gccaggcagt gggattgtgg
    11101 tgaatatcct gtttaaaatt aacggcacca ccgaaattca gtaagcagaa agtcaaaagc
    11161 ctccgaccgg aggcttttga ctattactca acaggtaagg cgcgaggttt tccttcagga
    11221 tcaaccgcga catacttaaa taatgcgtct gtcgctttat agcgttgccc gattggttca
    11281 gacgctactt ttttcaccca cacttcaata ttaatgctga ccgatgtcgt ccctttctgg
    11341 acacagcgtg catagcagca caccacatcg ccgaccgcaa ccggccgtaa gaaagtcatt
    11401 ccttcaaccc gcacagtcac tacgcgaccg tgggcaattt cttttgccag aatagcgccg
    11461 ccaatatcca tttgtgacat taaccaacca ccaaagatgt caccattggc attggtatcg
    11521 gcgggcatgg ctaaagtacg taaaacaaga tcgccctgag ggacgttatg tgttgtagac
    11581 atggtaaaac cgacttaaaa ggaaaatcac aggcgcgatg ctactatgat tttcctgagg
    11641 agaacagagg agatacagcg ccagccccaa aggactggca gtctggttag gatttatctt
    11701 cctgcggcat gtggcggtag atatagatac cgcttaacaa tgtgaagatt aaggtaaggg
    11761 cggtcaggcc aaagacttta aagttgaccc aaatattttg cggcagccag aatgcgatgt
    11821 agatgtttgc cagaccgcaa aggataaaga aaacagccca ggccagattc agcttcgacc
    11881 ataccggttg cggcagcgtg agttctttac ccagcatccg ctgaattagc ggctttttca
    11941 tcacccattg gctgactaac agggctcccg caaacagagc ataaatgact gtaaccttcc
    12001 atttaataaa ctcatcattg tggaagaaca gcgtcaagcc accgaagacg accaccagaa
    12061 caaaagtgat cagggccatc ttctcaacct tacgaaagcg aacccagcta tagataagca
    12121 caatcgccgt ggcgacgatc agcgccgcag tagccgcata gatgtcataa attttgtaaa
    12181 acgcgaaaaa gacaaccagc ggtaaaaaat caagaaactg cttcatttta cgattccgta
    12241 atcaaatgct taaggttatt ggcgaatcaa catatacagg cggaacagat agatgagcaa
    12301 tatggctgaa atcaggttgc tcaaggtgtt cgccagtacg gcaccaattt ccggggttaa
    12361 tgcggcaaaa gaagaggcaa aaagcagcaa cagtgttttt gccagcaacc agctcagtac
    12421 tgcgggtgcc accagacgca tattcgccca agtcagccgc atactgctac ggatcgaggc
    12481 aaaaacgccc attttgtcct gtaccagcat caccggtgcc agggccagta atatggccat
    12541 gataattccc ggaacgacca ccagcatgat gccaatctgt actaaaaggg tagtcagaaa
    12601 aatcagaata aataactttg gcaatatcgg cgcactggca ccaatagctc gcagcgcact
    12661 gactctctga cccgcagaca ccagctggat aattaatatt acgcctccgg cgagaatggc
    12721 gttaccgatt aatcctgaaa aagtggacgc cgctgaagcc tgcagcaaaa tttgttgctg
    12781 ttccggtgac atattctgaa ccaggtcgaa caacccacta ctgccgctaa cgggcacgcc
    12841 gtcattgagc tgcgcaagct gtgcatcact gggtgagaaa acatgcccta acaccactgt
    12901 gataaacgca catagcaacg ataccaacag aatggtcata aattgattac ggaagaaatt
    12961 tccggtgtca cggtatacgg actgcgccgt gatagacatg cactctcctt gagttttatg
    13021 caggtgttaa ttagcgggca attgtaccct ggttaagcct cgggtggcag catcaggtct
    13081 tgtatggaaa agcatatctt tgtaaagcgg aggtaattgc tggccttcag gcgtgggcaa
    13141 accgcggcaa gtggcgaacg agttaatatt tgcgtagcga aaatatttaa aaattgattt
    13201 aaatcacgtt aaccaggatt ctcaatgcaa cttctaaatt aatccagatc aataaatggt
    13261 gaattatcat atgtaatgtg atctatgtag gatcatttgt tactccaatg taggtatatt
    13321 cgtcacgttt ttataaccat aacgacggag cggatatgaa aaagttaaca gtggcggctt
    13381 tggcagtaac aactcttctc tctggcagtg cctttgcgca tgaagcaggc gaatttttta
    13441 tgcgtgcagg ttctgcaacc gtacgtccaa cagaaggtgc tggtggtacg ttaggaagtc
    13501 tgggtggatt cagcgtgacc aataacactc aactgggcct gacgtttact tatatggcga
    13561 ccgacaacat tggtgtggaa ttactggcag cgacgccgtt ccgccataaa atcggcaccc
    13621 gggcgaccgg cgatattgca accgttcatc atctgccgcc aacactgatg gcgcagtggt
    13681 attttggtga tgccagcagc aaattccgtc cttatgttgg ggcaggtatt aactacacca
    13741 ccttctttga taatggattt aacgatcatg gcaaagaggc ggggctttcc gatctcagtc
    13801 tgaaagattc ctggggagct gccgggcagg tgggggttga ttatctgatt aaccgtgact
    13861 ggttggttaa catgtcagtg tggtacatgg atatcgatac cacggctaag tacaaatctg
    13921 gtgttactac cgtaaaagac tcggtacgac tggatccgtg ggtgtttatg ttctccgcag
    13981 gatatcgttt ttaattttct ctgcaaaacc tctgcaaaac ccctctgcaa aactggtcat
    14041 caaatgacca gttttttcca ttccttaccg cgtgcgtcgt tgtaaatatc ggtcattttt
    14101 tgattcgaat ggcctagcaa aattttggta tcaaccccct gctctctgaa caatcgctct
    14161 gataaagatc tctgctcatg gaaagaggg
//
LOCUS       DAZFAB010000112        14187 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZFAB010000112 DAZFAB010000000
VERSION     DAZFAB010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14187
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.112"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..2092)
                     /locus_tag="ACHJWA_003863"
     CDS             complement(<1..2092)
                     /locus_tag="ACHJWA_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFO4310375.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASVHAQTVEKSAQAHERMAREVEQTRQRMEALSQK
                     MREEQAQAMALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGIGQQ
                     DYLALISEVTAKTRVLTQAEEEATRQKVAFIRQLKEQATRQNLSSSELLRAKAAQLGV
                     SSAAEVYIRKMEQAGKATHSLGLKSAAARQEIGVLIGELARGNLGALRGSGITLANRA
                     GWIDTLMSPKGMMPGAVIGGIAAAVYGLGKAWYDGQKEGEEFNRQLSLTGHYAGVTAG
                     QLWTLSRAISGNGITQHAAAGALAQVVGSGAFRGNDIGMVARAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNELHFLTATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     RRTADIDNNLNALGSTLKYLSDLWSRFWDAAMNIGREDSLDEQIAALQEKVSRAKRLP
                     WTASSSQVEYDQQRLNDLQEKKRQKDLQDAKEQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQREDERYEKALASGKKKTRETRNDEATRLLL
                     QYSQQQAQVEGQIAAARQSAGIATERMTEARKQLLALQQRISDLDGKKLTADEKSVLA
                     RKDELIQALTLLDVK"
     gene            complement(2064..2393)
                     /locus_tag="ACHJWA_003864"
     CDS             complement(2064..2393)
                     /locus_tag="ACHJWA_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO4310376.1"
                     /translation="MQFVMRLAREFRRADWRRMLSEMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            complement(2402..2788)
                     /locus_tag="ACHJWA_003865"
     CDS             complement(2402..2788)
                     /locus_tag="ACHJWA_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO4310377.1"
                     /translation="MFLKQGTFNYEKQSVVLSELSGLQRIEYLAFVQQRTAKFDAEEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASVITTWSYDALGAGAEM
                     VLSLSGMGAIENAGDLEHEVLTPEKS"
     gene            complement(2849..3592)
                     /locus_tag="ACHJWA_003866"
     CDS             complement(2849..3592)
                     /locus_tag="ACHJWA_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4310378.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASASVNVGATTTLTFTVKPDGASDKAISVHSTDPQTATVTLNGLVATVKG
                     VKQGSVSIVGMTADGNFVAVAAVTVSAAG"
     gene            complement(3603..4004)
                     /locus_tag="ACHJWA_003867"
     CDS             complement(3603..4004)
                     /locus_tag="ACHJWA_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO4310379.1"
                     /translation="MNRHTQIRQVVLARLREQCGDSATFFDGLPAFVDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDVSALSGLID
                     TLIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            complement(4001..4579)
                     /locus_tag="ACHJWA_003868"
     CDS             complement(4001..4579)
                     /locus_tag="ACHJWA_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4310380.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAGSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLGTARVRLAQR
                     GGKLQYRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIPLSGPLTQ
                     AFEDARDRIIAAEMPKQLGYALKQQLRLWLTR"
     gene            complement(4591..4866)
                     /locus_tag="ACHJWA_003869"
     CDS             complement(4591..4866)
                     /locus_tag="ACHJWA_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HFO4310381.1"
                     /translation="MSDPFSRLAARMDAITVRKMGKTASINDVDMTVIPGETLAELNA
                     LSGPAVSLVVFSSGYRPRRGDRVVYDGQQWTVTRHERFNGKPMIFIE"
     gene            complement(4859..5182)
                     /locus_tag="ACHJWA_003870"
     CDS             complement(4859..5182)
                     /locus_tag="ACHJWA_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HFO4310382.1"
                     /translation="MAKNFVEEGKTVAIVASAAISSGDLVQVGDVFAVALTDIPQGET
                     GDGMTEGVFMLPKLKTDDMKTGKKVYLKSGKVQLTNSGSDPLVGVVWADAGTSAEEVP
                     VKLNV"
     gene            complement(5269..7296)
                     /locus_tag="ACHJWA_003871"
     CDS             complement(5269..7296)
                     /locus_tag="ACHJWA_003871"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HFO4310383.1"
                     /translation="MPQRNDRSRSTPTTSPKNNSWFRMQAGHQSDADIYIYDEIGFWG
                     VTAKQFISDLNALGDITHINLNINSPGGDVFEGIAIFNALKTHGASITVYVDGVAASM
                     ASVIAMVGKPVIMPENSFMMIHKPFGFTGGDAEDMRTYADLLDKVEAVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAHGFADQVTPAVKAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRVQDDGPAASRTPVQAAAPVVDENSIRAQVLAEQKARVNGINDLFAMFG
                     GRYQTLQAQCLADPECSLEQAREKLLNEMGRESTPSNKNTPAHIYAGNGNFVGDGIRQ
                     ALMARAGFEKTERDNVYNGMTLREYARISLTERGIGVSSYNPMQMVGAAFTHSTSDFG
                     NILLDVANKAILQGWEDAPETYEQWTRKGQLSDFKIAHRVGMGGFSALRQVREGAEYK
                     YVTTGDKQATIALATYGELFSITRQAIINDDLNMLTDVPMKLGRAAKSTIADLVYAIL
                     TSNPKISTDNVSLFDKAKHANVLESAAMDVASLDKARQLMRVQKEGERHLNIRPAFVL
                     VPTAMESVANQVIRSSSVKGADINAGIINPVKDFATVIAEPRLDDNSQTTFYLAASKG
                     SDTIEVAYLNGVDTPYIDQMEGFSVDGVTTKVRIDAGVAPVDHRGLVKCTA"
     gene            complement(7241..7576)
                     /locus_tag="ACHJWA_003872"
     CDS             complement(7241..7576)
                     /locus_tag="ACHJWA_003872"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4310384.1"
                     /translation="MASGVIRLPRDLDRSSLYTAVYSGPVMPWIDPVKEAEAWKIQIR
                     GGAATESDWVRAGGRNPDDVKRRRKAEIDENRKLDLVFDTDPASDKGGSSAATKRQEP
                     QHTDDQSEE"
     gene            complement(7698..8822)
                     /locus_tag="ACHJWA_003873"
     CDS             complement(7698..8822)
                     /locus_tag="ACHJWA_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4310385.1"
                     /translation="MGAPPCGSDYTTTGASRVPAGEVLMAILDDVIGVFSPGWKAARL
                     RSRAVILAYEAVKTTRTHKARRENRTADQLSQYGAVSLREQARYLDNNHDLVIGVFDK
                     LEERVVGKNGIIVEPHPVLRNGAIARDLAAEIRTRWSEWSVSPEVTGQFTRPMLERLM
                     LRTWLRDGEVFAQMVSGRINSLTPSAGVHFWLEALEPDFIPMTSDESNRLNQGVFVDD
                     WGRPEKYLVYKSRPLSGRQMETKEVDAERMLHLKFVRRLHQMRGTSLLSGVLIRLSAL
                     KEYEDSELTAARIAAALGMYIRKGDGQSYEPDGNGSKDKERELTIQPGIIYDDLKPGE
                     EIGMVKSDRPNPNLETFRNGQLRAVAAGSRLNFFQYSAQL"
     gene            complement(8750..8962)
                     /locus_tag="ACHJWA_003874"
     CDS             complement(8750..8962)
                     /locus_tag="ACHJWA_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310386.1"
                     /translation="MNQNDIEAMIQRYTEAEMAVLDGKSVTFNGQQMTMENLSEIRQG
                     RQEWERRLAALITRRRGHPGYRLARF"
     gene            complement(8959..11061)
                     /locus_tag="ACHJWA_003875"
     CDS             complement(8959..11061)
                     /locus_tag="ACHJWA_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO4310387.1"
                     /translation="MLNQETAKAARTDSGYILRAPRRMRVADAVAQYMRVPMGAGNSV
                     PWDPLVAPYVIEPMNCLASREYDAVIFVGPARTGKTIGLIDGWVIYNVICDPADMLII
                     QMTEEKAREHSKKRLARTFRVSPEVVSRLSPNKNDNNVYDRTFLAGNYLKIGWPSVNI
                     MSSSDYKCVALTDYDRFPEDIDGEGDAFSLASKRTTTFMSSGMTLVESSPGRDVKDVK
                     WRRTSPHEAPPTTGILSLYNRGDRRRWYWPCPHCGEYFQPCGDVVAGFRDIADPVLAS
                     EAAYIQCPSCSGRIMPEQKRELNGRGVWLRDGESINADGSRYGDPRRSRIASFWMEGP
                     AAAYQTLSQLVYKLLTAEQEYETTGSEETLKTVINTDWGLPYLPRASMEQRKSELLEQ
                     RAEPVPSSSVPDGVNFLVATVDVQAGRHRRFVVQVTGYGSRGERWIIDRYNITQSLRG
                     DSDGESQRIDPASYPEDWDVLLTDVFHKSWPLASDPSQQMRLMAMAVDSGGEDGVTDN
                     AYKFWRRCRRDGLGKRIYLFKGDSIRRAKLITRTFPDNTGRTGRRAQAAGDVPLWLLQ
                     TDALKDRVNNALWRDSPGPGYVHFPDWLGSWFYDELTYEERSSDGKWSKPGRGANEAF
                     DLMVYAEALVILHGYEKIRWPDAPGWASRETWLESVPDSTEPSPSPEPVSTPVKKQKR
                     KKTVTDDVNPWLTSGGWL"
     gene            complement(11061..11552)
                     /locus_tag="ACHJWA_003876"
     CDS             complement(11061..11552)
                     /locus_tag="ACHJWA_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFO4310388.1"
                     /translation="MDHELKNLVLNINQLAALSGLHRQTVVARLKNIRPAGGHDKLKL
                     YRLTDILTEFMGLPPPVAEGEMDPHERKAWYQSERERLKFEQETAQLIPASDVRREFA
                     IWAKAVVQVLETLPDILERDCGLQPAAVSRVQSIIDDLRDQIALRVTEAGADDEEELQ
                     QEE"
     gene            complement(12227..12379)
                     /locus_tag="ACHJWA_003877"
     CDS             complement(12227..12379)
                     /locus_tag="ACHJWA_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310389.1"
                     /translation="MPSMIAIILLIILHIWLCRQGGAHFWSESWLNISLLMLDIEHLA
                     RGKGLR"
     gene            complement(12367..12834)
                     /locus_tag="ACHJWA_003878"
     CDS             complement(12367..12834)
                     /locus_tag="ACHJWA_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO4310390.1"
                     /translation="MSRVTAIISALVICIIVCQSWAVNHYRDNAIAYKEQRDKKVSEL
                     KQATATITDMQQRQRAADALDAKYTKELADAKAKNDALRRKLDNGGRVFVKGKCPVPS
                     SDETSSASGMGNDATVELSPVAGRNVLGIRDGIIRDQTALRTLQEYIRTQCLR"
     gene            complement(12831..13328)
                     /gene="rrrD"
                     /locus_tag="ACHJWA_003879"
     CDS             complement(12831..13328)
                     /gene="rrrD"
                     /locus_tag="ACHJWA_003879"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFO4310391.1"
                     /translation="MSPSLRKAVAAAIGGGAAAIASVLITGPSGDDGLEGVSYIPYED
                     IVGVWTVCHGHTGKDIIPGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            complement(13328..13543)
                     /locus_tag="ACHJWA_003880"
     CDS             complement(13328..13543)
                     /locus_tag="ACHJWA_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO4310392.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            13686..14084
                     /locus_tag="ACHJWA_003881"
     CDS             13686..14084
                     /locus_tag="ACHJWA_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310393.1"
                     /translation="MLHIISPRGISPRQRRLFYPFSSALALFYYAAAFTSGTMLSIST
                     QYVVLLVTNIITINRHRISPVYRNRVKKPEPESNHRMTICQWQVIKKRPRHAQPELTR
                     KMIEGNNISDVRMAPPAKFCNDQTELATCP"
     gene            14099..14187
                     /locus_tag="ACHJWA_003882"
                     /pseudo
     CDS             14099..14187
                     /locus_tag="ACHJWA_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311526.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3250 a         4193 c         3286 g         3458 t
ORIGIN      
        1 gttttacatc cagcagcgtc agtgcctgaa tcagttcatc tttacgggcc agcacactct
       61 tttcatctgc cgtcagtttt ttcccgtcca ggtcgctgat gcgctgctgc agagccagaa
      121 gctgtttacg cgcttctgtc atcctttccg tggcaatgcc tgctgactgt ctggcagcag
      181 caatctgtcc ttccacctgt gcctgttgct gactgtactg cagcaataac cgggtggcct
      241 catcattacg ggtttcgcgt gtttttttct taccggatgc cagggctttc tcgtaacgtt
      301 catcttcacg ttgtatcgcc gcatccctga cagcctgatc ggcgtactgc atggcattaa
      361 tacgcgctat ttcacgctga tgtcgtgctg cttccgtttc attcatccgg ttcagtgcag
      421 cattttcagc attacggcgt ttctgttgct cctgataatt ccgctctgcc tgctcttttg
      481 catcctgcaa atccttctgg cgttttttct cctgaagatc gttaagacgc tgctgatcgt
      541 attcaacctg agaagatgat gccgtccagg ggagtctttt cgcccgcgac actttctcct
      601 gtaaagcggc aatctgttca tccagcgagt cttcacgacc aatattcatg gccgcatccc
      661 agaaacgact ccacaaatca gacagatact tcagcgtact gccaagcgca ttgaggttat
      721 tatcaatatc cgcagtacgc cgaccggttt cctctgccag tgcagacatg gctatccgtg
      781 cagcatcact ggaccgcccc tgctccccaa ggacgcgtat ctgctcaagc tgagtggcag
      841 taagaaaatg cagctcattg tccagagcct tcgcggcatt tacaggatca tccttcagcc
      901 gcttaaactg atttatggta tcgctgaccg actggccaac cgatcgctcc atctgtgcgg
      961 cagctctcgc caccataccg atatcgtttc cacgaaatgc accactcccc accacctgag
     1021 ccagcgcacc ggctgcagca tgttgcgtga taccattccc ggaaatagca cgactgagcg
     1081 tccacagctg cccggcagtg actccggcat aatgccccgt cagcgacagc tggcggttaa
     1141 attcttcccc ctccttctga ccgtcatacc aggctttacc cagaccatag acagccgcgg
     1201 caataccgcc aataaccgct cctggcatca tgcctttcgg tgacatcagt gtgtctatcc
     1261 acccggcacg gttagccagc gttattccgg atcccctcag cgcacctaaa ttgccgcggg
     1321 ccagttcacc tatcagaacg cctatctcct gacgggctgc tgcacttttc agacccagcg
     1381 aatgcgtggc ttttcctgcc tgctccattt tgcggatata cacttctgca gcactgctta
     1441 cccccagctg ggcagcctta gcacgaagca actcagaaga agaaagattc tggcgggttg
     1501 cctgctcttt aagctgacgg ataaacgcca ctttctgtcg ggtagcctct tcctcagcct
     1561 gtgtaagaac acgggttttc gccgtaacct cagaaatcag cgccagataa tcctgctgac
     1621 caatcccgcc actgtttctg gcctgtcgga tctgttgctg aatacgctgt aattcctgca
     1681 gccccgcacc ggcctgtttc acactgtcaa tctgacgata aaacgcagca gccgctttat
     1741 cctgagcctc cgccagagcc atggcctgcg cctgttcctc gcgcattttc tggctcagtg
     1801 cctccatacg ctggcgggtt tgctccacct cgcgggccat gcgttcatga gcctgtgcgc
     1861 tcttctccac cgtctgcgca tggacggatg cggctgttgc agccgaagaa gccgcctgca
     1921 ttgtctgccg ggcggcctga gtctgacgct ccataaaacg ctgcatacgg gcagaagacc
     1981 gttctgcatc gctggctgca ccattcagaa ggtttttgat acggggaatt tcatttttaa
     2041 actctgccgc atcaatcccc aaatcaatga ccaggttggc tatctggtcc ataacgcaca
     2101 cctccggaaa taccttcccc aagatgcatc agttcttcgt ccgttcgctc cggtatcccg
     2161 ttctcttccg gtaaaaggct gaaatcagcc accgcagcat cactgctacc ggacaccatt
     2221 ctcacgatca atgccttcag cgaggcaaac tgcgcatcca tccacacatc actgaagctc
     2281 tgcatccgga aataatcgcc ccactcacca agctcagtgg ccgacatttc cgacagcatc
     2341 cgccgccagt ctgcccgccg gaactcccgg gcaagccgca tgacaaactg catttcccgc
     2401 gtcaggactt ttccggcgtc agcacctcat gctccaaatc cccggcattc tcaatggctc
     2461 ccataccgct cagcgacaga accatctccg cccccgctcc cagggcatca tacgaccatg
     2521 ttgtaataac ggatgcgcaa agcgtctcaa catcctgaga ctgttccgca ttccacagtg
     2581 agcgggaaac cagccaggca ttgatatcca tccccatccg cagaaaagca atctgtcgtt
     2641 cagcctccgg cagttctccc tcttcggcat caaactttgc cgttcgctgc tgaacaaacg
     2701 ccagatattc aattctctgc agcccggaca gctcactgag caccacggac tgcttttcat
     2761 aattaaacgt gccctgtttc agaaacatca tgttctccac ctgcaaaaaa gccccggata
     2821 accggggcaa atgatgagta tcgtcctgtt aacctgcagc gctgacagtc accgcagcca
     2881 cagccacaaa attcccgtca gcggtcatgc ccacaatgct gacgctgccc tgcttcacgc
     2941 ctttcaccgt ggccacaagt ccgttcaggg tcacagtggc tgtctgtgga tctgtcgaat
     3001 gcacactgat cgctttgtca ctggctccgt caggttttac tgtaaaggtc agcgtggtgg
     3061 ttgctcccac attgacactg gcggatgccg gtgccaccgt cagcccggta acgcccacag
     3121 tttcagtgcc ctcctctgcc agatatggac gccccacacc gctgattttc actgtgcggg
     3181 tcatcacgtc ttttgaggca atggttttac ccagtgagct cagccagccg cggaaaacat
     3241 caacagtgcc gttgggatat ttgatacgaa acgcgcagac ttcaccggag tcgaacaact
     3301 gaaccagttt tttctgcccg ctgtcacccg gacgccaggc cagcgtcgcc gaagtatcac
     3361 caacggattt ctgcccctgg gttgtcgttt tccagtctgc atcttcatca tcgagataag
     3421 tgtcatcttc tgcatcagcg gtcatttcgc caggttgcag atccttcacc atcgcaagac
     3481 gcagccagtc agtgtccgac aaagggttcg caaacgcatc gccgttgccg gtatacatcc
     3541 agaacgtcgt ccccgcacct ttcgtctttg ccagtggatt tggcgtggtc attaccacct
     3601 ccttaattcg tgtacgtgat ctggtacgtg atttccgcca tcgcccaggt ggccatctca
     3661 ttatcacgtt gatagttaaa accgagtggg atcagggtgt cgatgagtcc ggaaagtgcc
     3721 gatacatcat tcagggccgg gaaaatggtg ctctccatcc acatatccag ctctgaatcc
     3781 ggtgcctgtg cccggatgaa gacagcaata tgcagaacag cctgccagtc atcttcatcc
     3841 gtcatttttc cggtgtactg agcatcactc agccacaccg ccacggcagg cagttcctgc
     3901 gcatcaacaa atgccggaag cccgtcaaaa aacgtggcgc tgtctccaca ctgttcccga
     3961 aggcgtgcca gtacgacctg gcggatttgt gtatgtcggt tcatcgggtc agccataacc
     4021 tcagttgttg tttcagtgca taccccagct gtttcggcat ttccgcagca atgatgcggt
     4081 cgcgggcatc ttcaaatgcc tgtgtcagcg gtccggacag cgggattttc accacatcaa
     4141 tggggtaacg atttttgcca tcaatacgcc gcatcacatg ccagcgacca ttcgccagtt
     4201 gctgaataaa cgcatcccgg aaaagatatt tacccacctt cagcacactg ccacggtact
     4261 gcagttttcc accacgctgg gccagtctga cccgggctgt ccccagctta atggcgggca
     4321 gattgccccg gttaacgcgg atcctggccg tcatttttcc tgacggactg gctttaaaca
     4381 cccggacacg ctgacgaacc agtttcaggg ggatcccttt cacctggtta tctcccgcaa
     4441 cggtattccc ggcaacctgc cgggtggcaa ccgagcccgc tttctgtgcc acacggttta
     4501 tcgcccatgc gctggcctgt ggcaccatac gggtatcaag gctgttcaga ttgcggatgg
     4561 cattctcaag ccccttcatc ccacaccgct ttactcaata aagatcattg gcttaccgtt
     4621 aaagcgttca tgccgtgtga ccgtccattg ttgtccgtca taaacaacgc gatccccgcg
     4681 ccgtgggcgg tatcccgaag aaaacaccac cagagagacc gcaggtccgg acagagcatt
     4741 cagctctgcc agtgtttctc ccgggatcac agtcatatcg acatcattaa tcgaggctgt
     4801 ctttcccatc tttctgaccg tgatagcatc catacgcgct gccagccggg aaaagggatc
     4861 agacattgag ttttaccggc acttcttctg cactggttcc ggcatctgcc cagacaaccc
     4921 cgaccagcgg atcagagccg ctgttagtca gctgaacttt tcctgacttc agataaacct
     4981 tcttacccgt tttcatgtca tccgttttca gcttaggcag cataaacaca ccttcggtca
     5041 tgccgtcgcc tgtttcaccc tgtggaatat cggtcagcgc caccgcaaaa acatcgccca
     5101 cctgcaccag atctccgctg ctgatggctg cactggcaac aatcgccacc gtttttcctt
     5161 cttctacaaa attctttgcc ataactgtct ccgcacagcc ccgttcaggg gctgatttca
     5221 ggtacaaaaa aagcccttac gggccatcag agttgttgtc tgcgacgttt acgccgtaca
     5281 tttcaccaga ccgcggtgat caactggcgc gacaccggcg tcaatacgca ctttcgttgt
     5341 cacgccatcc acactgaagc cctccatctg atcaatatat ggcgtatcca caccgttgag
     5401 ataagccact tcaatcgtat cggagccttt tgacgcagcc aggtagaagg tggtctggct
     5461 gttatcatca agacgaggct ctgcaataac ggtcgcaaaa tctttcaccg ggttaataat
     5521 accggcgtta atgtcagccc ccttgacact tgaggagcga atgacctggt tagcaacaga
     5581 ctccatcgcc gtcggtacca gtacgaacgc aggacgaata ttcagatgac gctccccctc
     5641 tttctgaacg cgcatcaact ggcgggcttt atccagcgat gccacgtcca ttgcagcgct
     5701 ctccagtacg tttgcatgtt tcgctttatc gaacagactt acattatctg tggagatttt
     5761 cgggttagac gtcagaatgg cataaaccag atcggcaata gtggatttcg ccgcacggcc
     5821 cagtttcatc gggacatcgg tcagcatatt cagatcatca ttgataatgg cctgacgggt
     5881 gatactgaac agctcgccat aggtcgccag tgcaatagtg gcctgtttat ctccggtggt
     5941 gacgtattta tattccgccc cttcacgcac ctgacgcaga gcactgaagc cccccatacc
     6001 cacacgatgg gcaattttaa aatcagacaa ctgacctttc cgcgtccact gttcataggt
     6061 ttcaggggca tcttcccagc cctgcagaat ggctttgttc gcaacatcca gcagaatatt
     6121 accgaagtca gacgtactgt gtgtgaacgc cgcaccgacc atctgcatcg ggttataact
     6181 ggaaacccca ataccccgtt cagtcagtga aatacgggca tattcacgca gggtcatccc
     6241 gttgtagaca ttatcacgtt cggttttttc aaatccggca cgcgccatca gcgcctggcg
     6301 gatcccatcc cccacaaaat taccgtttcc ggcataaata tgagccgggg tatttttatt
     6361 agatggcgtg gactcgcgcc ccatctcgtt caacagcttt tcgcgggcct gctccagcga
     6421 acattcagga tcggcaagac actgagcctg cagcgtctga taacgcccgc caaacatggc
     6481 aaacagatca ttaataccgt ttacacgcgc tttttgctct gccagtacct gcgcacggat
     6541 actgttttca tccaccacag gtgctgctgc ctgcactggc gtccgggagg ctgcaggtcc
     6601 atcatcctgt acgcgtggag cactgttgcg tggcggagta atcatgtttc gaatggattc
     6661 cggcatcttt ttaaattcct ctgtacgttt tgactgaata catgccattg ccttaacggc
     6721 tggcgtcacc tgatcagcaa atccatgtgc cagacattcg gcaccggaca tccaggtctc
     6781 atccgccagc atggcagcaa tttcatcggt ggttttcccg gttttctgtg cataagcggg
     6841 taacagaacc gcctcaactt tatcgagcag gtcggcatag gtgcgcatgt cctccgcatc
     6901 accgcccgta aagccaaatg gtttatgaat catcatgaag gagttttccg gcataatgac
     6961 cggttttccc accatcgcaa tgaccgacgc cattgacgcc gccacaccgt cgacataaac
     7021 ggtaatggac gcaccatgtg ttttcagtgc attaaaaatg gcgatgcctt caaagacatc
     7081 gccacccggt gaattgatat tgagattaat gtgggtgata tcgcccagtg cattcagatc
     7141 actgataaac tgcttcgctg taacacccca gaaaccaatc tcgtcataaa tataaatatc
     7201 cgcgtcactc tggtgaccag cctgcatcct gaaccaggaa ttattcttcg gactggtcgt
     7261 cggtgtgctg cggctcctgt cgtttcgttg cggcactgct gcctccttta tcactggccg
     7321 gatcggtatc aaataccaga tccagcttgc ggttttcatc aatttcggcc ttgcgccgac
     7381 gtttgacatc atccggatta cgaccacctg cacgtaccca gtctgattct gtcgctgctc
     7441 caccacgaat ctgaattttc caggcctcag cctccttaac agggtcaatc cacggcatca
     7501 ccggtccgga atacaccgcg gtatacagtg aagaacggtc aagatcgcgg ggtagcctga
     7561 taacaccgga tgccacagcc tgtttcagcc aggcacgata catcgggcgg gtgacggcac
     7621 caataaacca gtcctgcagg atcaggtagc catcagtgga ttcaaccagc tcctgacgct
     7681 gggcgctgta agtgccgtta tagttgcgcg ctgtactgga aaaaattcag acgactgccc
     7741 gccgccacgg cacgcaactg accattacga aaagtttcaa ggttaggatt ggggcgatcc
     7801 gacttcacca ttccgatttc ttcgccgggt ttcagatcgt cgtaaataat gcctggctga
     7861 atggtaagct cgcgttcctt atccttgctg ccattaccat ccggttcata gctctgccca
     7921 tcgcctttcc ggatgtacat ccccagagca gcggcgatcc ttgctgcagt cagctcagaa
     7981 tcctcatact ctttcagggc actgaggcgg atcagcacac cggacaacaa agacgtcccg
     8041 cgcatctggt gcagacggcg aacaaattta agatgcagca ttcgctctgc atccacttct
     8101 ttggtttcca tctgccgtcc ggataaggga cggcttttat acaccagata tttttcggga
     8161 cgcccccagt catcaacaaa cacgccctga ttcagcctgt tgctctcatc actggtcatg
     8221 ggaataaagt ctggctcgag cgcctccagc cagaaatgaa caccggcaga aggcgtcagg
     8281 ctgtttatgc gcccggaaac catctgggca aacacctcac catcgcgcag ccaggtacgc
     8341 agcatcagac gttccagcat cggacgggta aactgcccgg tgacttccgg actgacagac
     8401 cattcactcc atcgggtgcg tatctccgct gccagatcac gggcaatggc cccattgcgt
     8461 aataccggat gtggctcgac aataatcccg tttttcccca ccacccgttc ttccagcttg
     8521 tcaaatacac caataaccag atcgtggttg ttatcaaggt aacgggcctg ctcacgtaac
     8581 gacacggccc cgtactggct taactggtcg gcagttcggt tttcccgccg ggctttgtgt
     8641 gtccgcgtcg tttttacggc ctcataagcc aggatcaccg cacgggaacg cagccttgcc
     8701 gctttccatc ctggtgaaaa aacgccaatc acatcatcaa gaattgccat cagaacctcg
     8761 ccagccggta cccgggatgc ccccgtcgtc gtgtaatcag agccgcaagg cggcgctccc
     8821 actcctgccg tccctgccgg atctcagata agttttccat ggtcatctgc tgaccattaa
     8881 aggtgacgga ttttccgtcc agcaccgcca tttcagcttc cgtataacgc tgaatcatgg
     8941 cttcgatatc attctggttc ataaccatcc tccggaagtc agccaggggt taacatcgtc
     9001 agttactgtt ttcttccgtt tttgtttttt aacaggcgtg gataccggtt ccggtgaggg
     9061 tgacggttcg gtactgtccg ggacactctc cagccaggtt tcccggctcg cccaccccgg
     9121 tgcatccggc cagcggatct tttcgtatcc atgcagaatg accagagcct cggcatacac
     9181 catcaggtca aaagcttcgt tggcaccgcg acccggctta ctccatttcc cgtcactact
     9241 ccgctcttca tacgtcagtt cgtcgtaaaa ccagctcccc agccagtcag ggaaatgcac
     9301 atagccggga cctggagagt cacgccataa cgcgttattc acccggtctt tcagtgcatc
     9361 cgtctgaaga agccagagcg gcacatcacc tgcggcctgc gcccgtcggc ccgttcgtcc
     9421 ggtgttatca gggaatgtac gggtgatcag ttttgcgcgc cggatgctgt cgcccttaaa
     9481 caggtaaata cgtttaccaa ggccatcacg acggcaacga cgccagaatt tataggcatt
     9541 atcagtgacc ccgtcttcac cgccggagtc caccgccatt gccatcagtc gcatttgttg
     9601 agaaggatcg gaggccagcg gccagctttt atgaaaaaca tccgtcagca ggacatccca
     9661 gtcttccgga tagctggccg gatcaattcg ctggctctca ccgtcgctgt caccgcgcaa
     9721 tgactgcgtg atgttgtaac gatcaataat ccagcgttcg ccacggctgc catagcccgt
     9781 tacctgaacc acaaaacggc gatgacgtcc cgcctgcaca tccactgtcg ccacaaggaa
     9841 attaacgcca tccggcacac tgctggaagg aactggctct gcccgctgct caagcagttc
     9901 actttttcgt tgctccatgc tggcgcgggg aagataaggt aatccccagt cggtattgat
     9961 aaccgtcttg agtgtttctt cacttccggt tgtctcgtat tcctgttctg cagtaagcag
    10021 tttgtaaacg agttgcgaga gtgtctggta agcagctgcc ggaccctcca tccagaatga
    10081 cgcaatacgt gagcgtcggg gatcaccata acgactacca tccgcattga tggattcacc
    10141 atcccgcaac cagaccccac gtccgttcag ctcacgtttt tgttcaggca taatccgtcc
    10201 tgaacaggaa ggacactgaa tataagccgc ctcacttgcc agcacgggat cggcaatatc
    10261 acggaaacca gcaaccacat cgccgcaggg ctgaaaatac tcaccacagt gtggacaggg
    10321 ccagtaccag cgacggcgat cgccacggtt atagagcgac agtatccccg tggttggtgg
    10381 agcctcatgc ggtgaagtcc gtcgccattt cacatccttc acatccctgc cgggggaact
    10441 ctccaccagc gtcataccac tggacataaa tgtggtggta cgttttgagg caagagagaa
    10501 ggcatccccc tcgccatcaa tatcttccgg aaaacggtca taatccgtca gcgccacgca
    10561 tttataatct gatgaggaca tgatattgac tgacggccag ccgattttca ggtagttgcc
    10621 agcaaggaat gttctgtcat aaacgttgtt gtcatttttg ttcggactca ggcgactgac
    10681 cacttccggg ctgacgcgaa acgttctggc gagtcgtttt ttggagtgtt cgcgggcttt
    10741 ttcctccgtc atctgaatga tcagcatatc cgcaggatcg caaatcacgt tgtaaatcac
    10801 ccagccgtca atcaggccga tagtcttgcc ggttcgtgcc gggccaacaa atatcactgc
    10861 gtcgtattca cgcgaggcca ggcagttcat cggctcaata acatacggtg ccaccagcgg
    10921 atcccacggg actgagttcc cggcccccat gggcacccgc atatactgag caacggcatc
    10981 agcaacccgc attcgtctcg gtgcgcgaag gatataacct gaatcggttc gtgctgcctt
    11041 tgcggtttcc tgattcagca ttactcctcc tgctgtaatt cctcctcatc atccgcacct
    11101 gcttcggtca cccgcagggc tatctgatcg cgcagatcat caataatgga ctgaacacgg
    11161 ctcacagcgg caggctgcag accacagtca cgttccagaa tatccggtaa tgtctccagc
    11221 acctgcacga ccgcttttgc ccagatggca aactcccgtc tgacatcact ggccggaatg
    11281 agttgtgccg tttcctgttc gaacttaaga cgctcacgtt cagactgata ccaggctttg
    11341 cgttcatgtg gatccatttc gccctcagca accggcggtg gtaaccccat aaattcagtc
    11401 agaatatcgg tcaaccggta tagcttgagt ttgtcatgtc caccagcggg acgaatgttt
    11461 ttcagtcttg ccacgacagt ctggcggtgc agaccagata aagccgccag ttgattaata
    11521 ttcagcacca ggtttttcaa ctcatgatcc atatttcctc cggagagctt taaacatgca
    11581 tcgtgcgaac aactttaaga aaacgcgttc gatgtcgaac aaaaaacact caattcgaca
    11641 tacaaaaaac aaataaccat taataatcaa taagatgcaa agatgatggt ggccgataaa
    11701 aatgcaaaaa ctagcctttt tccgcgacgc tcccgccccg tggcaggcca ccccaccggg
    11761 aggagccgtc agcctgaaag ccatgacgaa cgtctgatac agcccttgca tgaatggcat
    11821 cgggataatc cagaaaggaa tagcatcgac ccacaagaat ctgtgtgagt gtcctgtttc
    11881 ttccaccccc gcacaggact ggcgagcatg agggacaaac ccgcgaatca ttagcgcggt
    11941 aaaaacccgg tgtgcatcgt ttttgattat tcccgcacac tcgcgcagaa ggagttcccc
    12001 gtcgggctac ggtcatggtt aatgcgggaa tacagcgacg atacagcgca tgatgtgtca
    12061 ggcttgaata cctttatccg ttaaaaggga tatcagttaa gttatcccgt gtagggtata
    12121 agccattatc aaagccactc tgtagggagt ggcttttgta atggcaataa aaaagccccg
    12181 cgaatgcgag gctaaatcca ggtattttta atgactggct cttatctcaa cgcagcccct
    12241 taccgcgcgc cagatgctca atatcaagca tcagcaatga gatgtttaac catgattcac
    12301 tccagaagtg agcaccaccc tgtctacaga gccagatgtg aaggatgatg agtaaaatta
    12361 tcgctatcat cgaaggcatt gcgtcctgat gtattcctga agcgttctca gtgctgtttg
    12421 gtcgcggata attccgtccc ggataccgag aacgtttcgt ccagcaactg gagagagttc
    12481 gacggtggca tcattgccca tgccggaggc gctggaggtt tcgtctgagg atggcacagg
    12541 gcattttcct ttgacgaaca cccgaccacc attatcaagc ttgcgccgaa gagcatcatt
    12601 tttagctttc gcatcagcta actccttcgt gtatttagca tcgagtgcat cagcagcacg
    12661 ctggcgctgc tgcatgtcag taatggtggc ggtcgcctgc ttcagctcac tgactttttt
    12721 atcacgctgt tctttatagg cgatggcgtt atcacggtaa tgattaaccg cccatgactg
    12781 gcagacgatg atgcagataa ccagagcgga gataatcgcg gttactctgc tcattgttgc
    12841 ccccacaaac agacttcacg ctcaatctcg cggcgagtca tcagcccttt ccattgctta
    12901 ccgccagcgt atgtccagcg ccgtagctga tcacatgcgc ctttgatatc accctggttt
    12961 attttgcgaa gaagcgtcga tgttctgaaa ttaccagcgc ccacgttgta aacgaacgag
    13021 taaagagcgc cgcgcgttgt ttccggtata tcgactttga tgtacgggtt aatttgtctg
    13081 gcgaccgtgg caaggtcttt attcaggagg gctttgcatt ctgcttcggt atacgtttta
    13141 ccgggaatga tgtcttttcc ggtgtgtccg tgacatacag tccatacgcc aacgatatct
    13201 tcgtatggta tgtagctgac accttccagg ccatcgtcac cactcggacc agtgatgagc
    13261 acagacgcta tggcagcagc cccaccacca atagcagcag caacagcctt gcgtaatgat
    13321 ggcgacatta ttcacctctc gcagccttac gcttatcttc tttaatcttg aaataaaggt
    13381 ttgtcagata cgtcagcaag ccaaacacca agctacccaa tacgcctatt gccacccact
    13441 gggatgggga gactttgtcc agcaactgca gtagccagta tcccgtcccc accgctgacg
    13501 tggtgtatga cacacctgtt gttatttttt ccatctggta cataccccgt ctcccgttat
    13561 ccggaagctg acaacaataa aaagccacca gttaattcct gatggccctg atgcataaac
    13621 gtcataatac ctgactgtta tgattgacaa taatgataat gtttatatag aaaggttccc
    13681 gatgtgtgtt acatatcatt tctccacggg gaatatcccc acgccagcgc agactctttt
    13741 acccgttctc ttctgcgctg gctctttttt attatgctgc tgcatttacc tctggcacca
    13801 tgctttctat ctcaacacaa tacgtggtac ttcttgtaac caatatcata acgattaatc
    13861 gacatagaat ttctcccgtg tacaggaaca gagttaaaaa gccggaaccg gaatcaaatc
    13921 acaggatgac catctgccag tggcaggtca taaaaaaaag gccgcgccat gcgcagccag
    13981 aactcacaag gaaaatgata gaaggaaata acattagtga tgtacgcatg gcgcctcccg
    14041 ctaagttctg caatgatcaa acagaactcg ctacgtgccc ttaaaactcg atcatttagc
    14101 ccctccaagg aggattcacc atgcggttga ttttttaata aacagtaaac aaaaaagtca
    14161 agaattattc attctgttct ttcatca
//
LOCUS       DAZFAB010000113        14111 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZFAB010000113 DAZFAB010000000
VERSION     DAZFAB010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14111
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.113"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            250..549
                     /gene="yoaC"
                     /locus_tag="ACHJWA_003883"
     CDS             250..549
                     /gene="yoaC"
                     /locus_tag="ACHJWA_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFO4310394.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(669..848)
                     /gene="yoaH"
                     /locus_tag="ACHJWA_003884"
     CDS             complement(669..848)
                     /gene="yoaH"
                     /locus_tag="ACHJWA_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFO4310395.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            922..2283
                     /gene="pabB"
                     /locus_tag="ACHJWA_003885"
     CDS             922..2283
                     /gene="pabB"
                     /locus_tag="ACHJWA_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFO4310396.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRRVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPAPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2287..2865
                     /gene="nudL"
                     /locus_tag="ACHJWA_003886"
     CDS             2287..2865
                     /gene="nudL"
                     /locus_tag="ACHJWA_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFO4310397.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            3049..4413
                     /gene="sdaA"
                     /locus_tag="ACHJWA_003887"
     CDS             3049..4413
                     /gene="sdaA"
                     /locus_tag="ACHJWA_003887"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO4310398.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     TANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4544..6142
                     /gene="pdeD"
                     /locus_tag="ACHJWA_003888"
     CDS             4544..6142
                     /gene="pdeD"
                     /locus_tag="ACHJWA_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO4310399.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTTDRVLIKGSPVLIQWTPAASSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFSFTINVNGPGAPALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(6146..7702)
                     /gene="yoaE"
                     /locus_tag="ACHJWA_003889"
     CDS             complement(6146..7702)
                     /gene="yoaE"
                     /locus_tag="ACHJWA_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFO4310400.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(7690..7848)
                     /gene="yoaL"
                     /locus_tag="ACHJWA_003890"
     CDS             complement(7690..7848)
                     /gene="yoaL"
                     /locus_tag="ACHJWA_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFO4310401.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            8165..9136
                     /gene="manX"
                     /locus_tag="ACHJWA_003891"
     CDS             8165..9136
                     /gene="manX"
                     /locus_tag="ACHJWA_003891"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFO4310402.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            9199..9999
                     /gene="manY"
                     /locus_tag="ACHJWA_003892"
     CDS             9199..9999
                     /gene="manY"
                     /locus_tag="ACHJWA_003892"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFO4310403.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            10012..10863
                     /gene="manZ"
                     /locus_tag="ACHJWA_003893"
     CDS             10012..10863
                     /gene="manZ"
                     /locus_tag="ACHJWA_003893"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFO4310404.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10918..11376
                     /gene="yobD"
                     /locus_tag="ACHJWA_003894"
     CDS             10918..11376
                     /gene="yobD"
                     /locus_tag="ACHJWA_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFO4310405.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLERIYKLLVSTQ"
     gene            11687..11809
                     /locus_tag="ACHJWA_003895"
     CDS             11687..11809
                     /locus_tag="ACHJWA_003895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310406.1"
                     /translation="MHINRYACMNAKTHRGLLFMYACLLGAMMCFMDTPVRTLS"
     gene            11806..12372
                     /gene="mntP"
                     /locus_tag="ACHJWA_003896"
     CDS             11806..12372
                     /gene="mntP"
                     /locus_tag="ACHJWA_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFO4310407.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(12369..13178)
                     /gene="rlmA"
                     /locus_tag="ACHJWA_003897"
     CDS             complement(12369..13178)
                     /gene="rlmA"
                     /locus_tag="ACHJWA_003897"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFO4310408.1"
                     /translation="MSFSCPLCHQPLTREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLNEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQKSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQILAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(13344..13553)
                     /gene="cspE"
                     /locus_tag="ACHJWA_003898"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(13344..13553)
                     /gene="cspE"
                     /locus_tag="ACHJWA_003898"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO4310409.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            complement(13566..13709)
                     /gene="yobF"
                     /locus_tag="ACHJWA_003899"
     CDS             complement(13566..13709)
                     /gene="yobF"
                     /locus_tag="ACHJWA_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFO4310410.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
BASE COUNT         3513 a         3513 c         3481 g         3604 t
ORIGIN      
        1 ccgttgtgtt cgccctttga ttagcaactc tggttttctt tcttagctga acaatctggt
       61 tatagcgtgc ggactgaccg ttaattttct ccatcttact ggataacaca ccatgagctg
      121 ctacctttaa aacacctgaa tatcaacagg ttaactcgtg ccatatcgtt ataatccctt
      181 tgatattaga tgcaaattaa ggtcatatat agccttatcg ataacatggt taattttaag
      241 gataagaata tgcctgctgt aatagataaa gccctggatt tcattggtgc catggatgta
      301 tcagcgccaa caccaagttc gatgaatgaa agcacggcga agggaatctt taaatattta
      361 aaagaactgg gagtacccgc gagtgccgct gatattacgg cgcgagctga ccaggaaggc
      421 tggaaccctg ggttcacgga aaaaatggtt ggatgggcaa aaaaaatgga gacaggtgaa
      481 cgctctgtga ttaaaaatcc tgagtacttt tcaacatata tgcaggaaga actaaaagca
      541 ctggtctgag ttaaatttat atcagcataa atgggtcagg acgcttttaa tcacatataa
      601 aaagcgtcct ttttctcaca atcaaataat catcagcttc tataacgaaa tcgatacgga
      661 ttagtgtgtt actcgtcttc atcctcaaaa cgcgccacga ttcgctcgcc ggagtggttg
      721 gcacgcagct cttctgccac cagcgcgatt gcctggccgc tgctcatccc ttgtgccatc
      781 agttcctgga tccgctcgac agctttttgc tgctgttcat gggtgagtga aggtaaacct
      841 gcaaacattg ttaactcctg ctaaattgtt ggcgctaatt atttcatgct acccggcaca
      901 tagccagtag agtcaggact gatgaagacg ttatctcccg ctgtgattac tttaccctgg
      961 cgtcaggacg ccgctgaatt ttatttctcc cgcttaagcc acctgccgtg ggcgatgctt
     1021 ttacactccg gctatgccga tcatccgtat agccgctttg atattgtggt cgccgatccg
     1081 atttgcactt taaccacttt aggtaaagaa accgttgtta gtgaaagcga aaaacgcaca
     1141 acgaccactg atgacccgct acaggtgctc cagcaggtgc tggatcgcgc agacattcgc
     1201 ccaacgcata acgaagattt gccatttcag ggcggcgcac tggggttgtt tggctacgat
     1261 ctgggccgcc gttttgagtc actgccagaa attgcggaac aagatatcgt tctgccggat
     1321 atggcagtgg gtatctacga ttgggcgctc attgtcgacc accagcgtca tacagtttct
     1381 ttgctgagtc ataatgatgt caatgcccgt cgggcctggc tggaaagcca gcaattctcg
     1441 ccgcaggaag atttcacgct cacttccgac tggcaatcca atatgacccg cgagcagtac
     1501 ggcgaaaaat ttcgccgggt acaggaatat ctgcacagcg gtgattgcta tcaggtgaat
     1561 ctcgcccagc gttttcatgc gacctattct ggcgatgaat ggcaggcatt ccttcagctt
     1621 aatcaggcca accgcgcgcc atttagcgct tttttacgtc ttgaacaggg tgcaatttta
     1681 agcctttcgc cagagcggtt tattctttgt gataatagtg aaatccagac ccgcccgatt
     1741 aaaggcacgc taccacgcct gcccgctcct caggaagata gcaaacaagc agaaaaactg
     1801 gcgaactcag cgaaagatcg tgccgaaaat ctgatgattg tcgatttaat gcgtaatgat
     1861 atcggtcgtg ttgccgtagc aggttcggta aaagtaccag agctgttcgt ggtggaaccc
     1921 ttccctgccg tgcatcatct ggtcagcacc ataacggcgc aactaccaga acagttacac
     1981 gccagcgatc tgctgcgcgc agcttttcct ggtggctcaa taaccggggc tccgaaagta
     2041 cgggctatgg aaattatcga cgaactggaa ccgcagcgac gtaatgcctg gtgcggcagc
     2101 attggctatt tgagcttttg cggcaacatg gataccagca ttactatccg cacgctgact
     2161 gccattaacg gacaaatata ctgctctgcg ggaggtggaa ttgtcgccga tagccaggaa
     2221 gaagcggaat atcaggaaac ttttgataaa gttaataaga tattacgcca actggagaag
     2281 taagacgtgg aataccgtag cctgacgctt gatgattttt tatcgcgctt tcaacttttg
     2341 cgcccgcaaa ttaaccggga aaccctaaat catcgtcagg ctgccgtgtt aatccccatc
     2401 gtccgtcgac cgcaaccggg gttgttgctg actcagcgtt caattcatct gcgtaaacac
     2461 gctggacaag tggcattccc tggaggtgca gtcgatgaca cggacgcatc agttatcgcc
     2521 gccgcgctgc gcgaagctga agaagaggtt gctataccgc cttccgccgt tgaagttatc
     2581 ggcgtgctgc cgcccgtcga tagcgtaact ggctaccagg taaccccagt ggtcggcatt
     2641 atcccgcccg atctgccgta tcgcgccagt gaagatgaag tttccgctgt gtttgaaatg
     2701 ccgctcgccc aggcattaca tctgggtcgt tatcaccctt tagatatcta ccgccgcggc
     2761 gattcacatc gggtatggct atcctggtac gaacagtatt ttgtatgggg aatgaccgca
     2821 ggcataattc gtgagctggc gctgcaaatt ggtgtgaaac cctgactata cttatcttta
     2881 catctacaaa acactacttg agacaatcat cgcaatatta gttaaatcgc ggtttttgat
     2941 tagtttaatt catgtgaata gttaagccag tcgccgcgtt ccctcttaca ctatgcgctg
     3001 ttattagttc gttactggaa gtccagtcac cttgtcagga gtattatcgt gattagtcta
     3061 ttcgacatgt ttaaggtggg gattggtccc tcatcttccc ataccgtagg gcctatgaag
     3121 gcgggtaaac agttcgtcga tgatctggtc gaaaaaggct tactggatag cgttactcgc
     3181 gttgccgtgg acgtttatgg ttcactgtcg ctgacgggta aaggccacca caccgatatc
     3241 gccattatta tgggtcttgc aggtaacgaa cctgccaccg tggatatcga cagtattccc
     3301 ggttttattc gcgacgtaga agagcgcgaa cgtctgctgc tggcacaggg acggcatgaa
     3361 gtggatttcc cgcgcgacaa cgggatgcgt tttcataacg gcaacctgcc gctgcatgaa
     3421 aacggtatgc aaatccacgc ctataacggc gatgaagtcg tctacagcaa aacttattat
     3481 tccatcggcg gcggttttat cgtcgatgaa gaacactttg gtcaggatac tgccaacgaa
     3541 gtgagcgtgc cgtatccgtt caaatctgcc accgaactgc tcgcgtactg taatgaaacc
     3601 ggctattcgc tgtctggtct cgctatgcag aacgaactgg cgctgcacag caagaaagag
     3661 atcgacgagt atttcgcgca tgtctggcaa accatgcagg catgtatcga tcgcgggatg
     3721 aacaccgaag gtgtactgcc aggcccgctg cgcgtgccac gtcgtgcgtc tgccctgcgc
     3781 cggatgctgg tttccagcga taaactgtct aacgatccga tgaatgtcat tgactgggta
     3841 aacatgtttg cgctggcagt taacgaagaa aacgccgccg gtggtcgtgt ggtaactgcg
     3901 ccaaccaacg gtgcctgcgg tatcgttccg gcagtgctgg cttactatga ccactttatt
     3961 gaatcggtca gcccggacat ctatacccgt tactttatgg cagcgggcgc gattggtgca
     4021 ttgtataaaa tgaacgcctc tatttccggt gcggaagttg gttgccaggg cgaagtgggt
     4081 gttgcctgtt caatggctgc tgcgggtctt gcagaactgc tgggcggtag cccggaacag
     4141 gtttgcgtgg cggcggaaat tggcatggaa cacaaccttg gtttaacctg cgacccggtt
     4201 gcagggcagg ttcaggtgcc gtgcattgag cgtaatgcca ttgcctctgt gaaggcgatt
     4261 aacgccgcac ggatggctct gcgccgcacc agtgcaccgc gcgtctcgct ggataaggtc
     4321 atcgaaacga tgtacgaaac cggtaaggac atgaacgcca aataccgcga aacctcacgc
     4381 ggtggtctgg caatcaaagt ccagtgtgac taatacttct tactcgccca tctgcaacgg
     4441 atgggcgaat ttatccccgc tttctcgtct gctgtaatat tccccactac acttccactg
     4501 ttgcgtcagg cgtttgtcgc catacgctta cagagtggcc cgcatgcaaa aagcacaacg
     4561 gatcattaaa acctatcgcc gtaatcgaat gattgtttgt acgatttgcg cactcgttac
     4621 gctcgcttcg accctgagcg tgcgatttat ttcacagcgt aacttaaatc aacaacgggt
     4681 agtacaattc gccaatcacg ctgtagagga attagataaa gtactgcttc ccctacaggc
     4741 aggtagcgaa gtcttgcttc cgctgattgg tctgccctgc tctgtcgccc atttgccatt
     4801 acgtaaacag gcggcaaaac tccaaactgt gcgatccatt ggcctggtgc aagacggcac
     4861 actttattgc tccagcattt ttggttatcg caatgtgccc gtcgtggaca ttctggctga
     4921 acttcctgca ccgcaaccac ttttacgcct gacgaccgac cgtgtcctga ttaaaggcag
     4981 tccggttttg attcaatgga cgcctgcagc gagcagtagc aatgctgggg tcatggagat
     5041 gattaacatc gacttactga cggcaatgct gcttgagcca caactgccgc aaatcagtag
     5101 cgccagcctg acggtggaca aacggcattt gctctatggt aatgggctgg tagattccct
     5161 tccacaacct gaagacaatg aaaactatca ggtttcttcg caacgctttt cttttaccat
     5221 taacgttaat ggtccggggg ctccggcgct ggcatggcac tatcttccaa cacaattacc
     5281 gctggcggta ctgctaagtt tactggtggg ctacatcgcc tggctggcga ccgcttaccg
     5341 aatgagcttt tcccgcgaaa tcaatctggg cctggcgcaa catgagttcg aattgttctg
     5401 tcagcctttg cttaatgcgc gcagccagca atgtattggt gtagagattt tgctacgctg
     5461 gaacaatccg cgtcagggct ggatttcacc ggatgtgttt attcctatcg cggaagaaca
     5521 tcatttaatt gtgccactga cccgctatgt gatggcagaa accattcgtc agcgccatgt
     5581 tttcccgatg agtagtcagt ttcatgttgg cattaacgtc gcacccagcc attttcgccg
     5641 tggtgtgctg ataaaagatc tcaatcagta ctggtttagc gctcacccga ttcagcaact
     5701 gatcctcgaa atcaccgaac gcgatgcctt actggatgtt gattatcgga ttgcccgcga
     5761 gctacatcgt aaaaacgtca aactggcgat tgatgacttc ggcaccggca acagttcatt
     5821 ttcctggctt gaaacattac gtcctgacgt gctgaaaatt gataagtcat ttaccgcagc
     5881 tataggttct gacgcggtta actcgacggt gaccgatatc atcatcgcgc tggggcaaag
     5941 actgaatatt gaactggtgg cggagggcgt ggaaacgcaa gaacaggcga agtatttgcg
     6001 ccgtcatggc gtgcatattt tgcaagggta tttgtacgca cagccgatgc cgctacgtga
     6061 ttttcccaaa tggctggcgg gcagccaacc gccgcccgcc cggcataatg gacatatcac
     6121 gcccattatg ccgttacgtt aacgcttact catcttcatc gtgcgccggt tgctctttaa
     6181 caatgcgaac cagatcaaca cgataatcat tggcttcaat gatggtgata tgcagtggcc
     6241 ctacatcaat cacatcgccc acacggggaa tgtgaccatt tgccgagatc acgaggcccg
     6301 cgaccgtcgc gatatcatcg tcatcggcaa ggtgctcaac atcaagcgcc tgctgcaagg
     6361 catgcaaatc tgtaccgcct tttaccagcc agccgtcacc atcagtaatg atttccggcg
     6421 tttcgtcagc gtccgggaat tcacccgcaa tggcttccag cacatccagc ggcgtgacca
     6481 agccttgtac cacaccaaac tcgttggtca cgataacaaa gctcccgcga gcacgacgca
     6541 gcacgcccag caggttgatc ggatcgaggg tttcagggac gataatcgcc ggagacgccg
     6601 aagcaatcgc cgccacatca acgccctctt ccagcgccac cagcagttct ttagcacgta
     6661 cgattccgat gatttcatcc agttcaccgc gacataccgg gaacaggctg tgcggtgagg
     6721 agagcagctg ctcgcggatt tcatcgaccc cgagattagc gtcaacccag cttatttcac
     6781 cgcgcggcgt catgatcccg cgcagagaac gcgacgccag cgtcagtacg ccgttaatca
     6841 tgtaacgttc ttcttcggca aatgcacctt ccgggatcgg catcggcatc gggttatcgg
     6901 catcgtgctg aacattggcc tgacgtttcc cgcccatcaa acgcaggatg gcatcggcag
     6961 tacgcgctcg cagcggcaaa gtcgactggt gacgaataaa gttgcgacgc gcaatctggt
     7021 taaacacttc gatgatgatc gagaagccaa tcgcggcata caggtaacct ttcggaatgt
     7081 ggaaaccgaa accttctgcc accagactca gaccaatcat taacaggaag ctcagacaga
     7141 gcaccaccac cgtggggtgc tggttaacga atcgcgtcag cggtttggat gccagcaaca
     7201 taaccgccat cgcaatcact accgccgcca tcatcaccgg cagatggtta accatcccta
     7261 ctgcagtaat taccgcatcc aacgagaaga cggcgtcaag gatgacaatc tgtgtgacga
     7321 ccacccagaa actggcgtag cctttaccgt ggccggaatc atgatcgcgg ttttccagcc
     7381 gttcatgcag ttcggttgtt gctttgaaca gcaagaatat ccccccgaac aacataatca
     7441 ggtcgcgtcc ggagaaggag aaatccatga cggtaaatag cggtttggtc agcgtgacca
     7501 tccatgaaat cagcgacagc agccccagac gcataatcag cgccagtgat aaccccagca
     7561 aacgcgcttt atcgcgttgt tttggcggca gtttgtcagc aagaatggcg atgaagacca
     7621 ggttatcgat acccagcaca atttcgagaa caacaagcgt gagtagcccc gcccaaattg
     7681 aggggtccat taagaattcc atgacaagct cctgcttaag gaatagctat tcgacgccag
     7741 aaataatgca ggcgtaacga caaaatgcaa acgaaaggtg cggcatagag tgccagaaag
     7801 gcaggcgtta aaaggcctga tgctgaaatg acgtcggtga cgatccatac tgcgggctac
     7861 tgccctatac tccatgattg ttaaacggga gttaaacata tcagagacgc ctctgatttg
     7921 gcaaagattt accttccttt gcaaacgaat gtgacaagga tattttacct ttcgaaattt
     7981 ctgctaatcg aaagttaaat tacggatctt catcacataa aataattttt tcgatatcta
     8041 aaataaatcg cgaaacgcag gggtttttgg ttgtagccct tatctgaatc gattcgattg
     8101 tggacgacga ttcaaaaata catctggcac gttgaggtgt taacgataat aaaggaggta
     8161 gcaagtgacc attgctattg ttataggcac acatggttgg gctgcagagc agttgcttaa
     8221 aacggcagaa atgctgttag gcgagcagga aaacgtcggc tggatcgatt tcgttccagg
     8281 tgaaaatgcc gaaacgctga ttgaaaagta caacgctcag ttggcaaaac tcgacaccac
     8341 taaaggcgtg ctgtttctcg ttgatacatg gggaggcagc ccgttcaatg ctgccagccg
     8401 cattgtcgtc gacaaagagc attatgaagt cattgcaggc gttaacattc caatgctcgt
     8461 ggaaacgtta atggctcgtg atgatgaccc aagctttgat gaactggttg cgctggcagt
     8521 agaaacaggc cgtgaaggcg tgaaagcact gaaagccaaa ccggttgaaa aagccgcgcc
     8581 agcacccgct gccgcagcac ccaaagcggc tccaactccg gcaaaaccaa tggggccaaa
     8641 cgactacatg gttattggcc ttgcgcgtat cgacgaccgt ctgattcacg gtcaggtcgc
     8701 cacccgctgg accaaagaaa ccaatgtctc ccgtattatt gttgttagtg atgaagtggc
     8761 tgcggatact gttcgtaaga cactgctcac ccaggttgca cctccgggcg taacagcaca
     8821 cgtagttgat gttgccaaaa tgattcgcgt ctacaacaac ccgaaatatg ctggcgaacg
     8881 tgtaatgctg ttatttacca acccaacaga tgtagagcgt ctcgttgaag gcggcgtgaa
     8941 aatcacctct gttaacgtcg gtggtatggc attccgtcag ggtaaaaccc aagtgaataa
     9001 cgcggtttcg gttgatgaaa aagatatcga ggcgttcaaa aaactgaatg cacgcggtat
     9061 tgagctggaa gtccgtaagg tttccaccga tccgaaactg aaaatgatgg atctgatcag
     9121 caaaatcgat aagtaacgta ttgtgttgat tatcagtcag ttttcacact ttagtcatac
     9181 gtaaacagga gaagtacaat ggagattacc actcttcaaa ttgtgctggt atttatcgta
     9241 gcctgtatcg caggtatggg atcaatcctc gatgaatttc agtttcaccg tccgctaatc
     9301 gcgtgtaccc tggtgggtat cgttcttggg gatatgaaaa ccggtattat tatcggtggt
     9361 acgctggaaa tgatcgcgct gggctggatg aacatcggtg ctgcagttgc gcctgacgcc
     9421 gctctggcat ctatcatttc taccattctg gttatcgcag gtcatcagag cattggtgca
     9481 ggtattgcac tggcaatccc tctggccgct gcgggccagg tactgaccat catcgttcgt
     9541 actattaccg ttgctttcca gcacgctgcg gataaggctg ctgataacgg caacctgaca
     9601 gcgatttcct ggatccacgt ttcttctctg ttcctgcaag caatgcgtgt ggctattccg
     9661 gccgtcatcg ttgcgctgtc tgtaggtacc agcgaagtac agaacatgct gaatgcgatt
     9721 ccggaagtgg tgaccaatgg tctgaatatc gccggtggca tgatcgtggt ggttggttat
     9781 gcgatggtta tcaacatgat gcgtgctggc tacctgatgc cgttcttcta cctcggcttc
     9841 gtaaccgcag cattcaccaa ctttaacctg gttgctctgg gtgtgattgg tactgttatg
     9901 gcagtgctct acatccaact tagcccgaaa tacaaccgcg tagccggtgc gcctgctcag
     9961 gcagctggta acaacgatct cgataacgaa ctggactaac aggtgagcga aatggttgat
    10021 acaactcaaa ctaccaccga gaaaaaactc actcaaagtg atattcgtgg cgtcttcctg
    10081 cgttctaacc tcttccaggg ttcatggaac ttcgaacgta tgcaggcact gggtttctgc
    10141 ttctctatgg taccggcaat tcgtcgcctc taccctgaga acaacgaagc tcgtaaacaa
    10201 gctattcgcc gtcacctgga gttctttaac acccagccgt tcgtggctgc gccgattctc
    10261 ggcgtaaccc tggcgctgga agaacagcgt gctaatggcg cagagatcga cgacggtgct
    10321 atcaacggta tcaaagtcgg tttgatgggg ccgctggctg gtgtaggcga cccgatcttc
    10381 tggggaaccg tacgtccggt atttgcagca ctgggtgccg gtatcgcgat gagcggcagc
    10441 ctgttaggtc cgctgctgtt cttcatcctg tttaacctgg tgcgtctggc aacccgttac
    10501 tacggcgtag cgtatggtta ctccaaaggt atcgatatcg ttaaagatat gggtggtggc
    10561 ttcctgcaaa aactgacgga aggggcgtct atcctcggcc tgtttgtcat gggggcattg
    10621 gttaacaagt ggacacatgt caacatcccg ctggttgtct ctcgcattac tgaccagacg
    10681 ggcaaagaac acgttactac tgtccagact attctggacc agttaatgcc aggcctggta
    10741 ccactgctgc tgacctttgc ttgtatgtgg ctactgcgca aaaaagttaa cccgctgtgg
    10801 atcatcgttg gcttcttcgt catcggtatc gctggttacg cttgcggcct gctgggactg
    10861 taagactgtt gtacactacc ggggcctttt ggccccgttt ttttatctgg aggattaatg
    10921 acaatcacgg acctggtact gattcttttc atcgccgcac tcctggcctt cgcgatctac
    10981 gatcagttca tcatgccccg ccgtaacggc cccaccctgc tggcaattcc tttgctccgg
    11041 cgtggtcgca tcgatagcgt tatcttcgtc ggattgattg tcattcttat ctataacaac
    11101 gtcacgaatc atggtgcgtt aataacgaca tggttattaa gcgcactggc tctgatgggt
    11161 ttttatatat tctggatccg cgttccgaag atcatcttta aacaaaaagg ttttttcttc
    11221 gccaatgtct ggattgaata tagccgaatc aaagcgatga acttgtcgga agatggcgtg
    11281 ttggtgatgc aattagaaca gcgtcggctg ttaatccgcg ttcgaaatat cgacgatctg
    11341 gaaagaattt ataaacttct cgtttcaact caatgagtta ttaatttagc caaagctatg
    11401 tttagtgtat ttttaataat cagacatagc ttaggctata ttacctcttc ccttatttgt
    11461 tatttattat aacgtttcat tgatatataa atctaaatga aaatcgttat caacaaagca
    11521 ataaaataat atattccaac agttgtttta tattctcaaa atatgttaag gttgcgccct
    11581 catttgggga gtagccaatt tccagattcc ggaaatgtac gtgtcaacat actcgttgca
    11641 aaacgtggca cgtacggact gaagactttc agtcaggcga gaccatatgc acatcaatcg
    11701 ctatgcctgc atgaacgcga aaacccatcg cggcttgttg ttcatgtatg catgtttgct
    11761 gggggcgatg atgtgtttta tggatacccc ggtcaggacg ttgtcatgaa tatcactgct
    11821 actgttcttc ttgcgtttgg tatgtcgatg gatgcatttg ctgcatcaat cggtaaaggt
    11881 gccaccctcc ataaaccgaa attttctgaa gcattgcgaa ccggccttat ttttggtgcc
    11941 gtcgaaaccc tgacgccgct gatcggctgg ggaatgggca tgttagccag ccggtttgtc
    12001 cttgaatgga atcactggat tgcgtttgtg ctgctgatat tcctcggcgg gcgaatgatt
    12061 attgagggtt ttcgtggcgc agatgatgaa gatgaagagc cgcgccgtcg gcacggtttc
    12121 tggctactgg taaccaccgc gattgccacc agcctggatg ccatggctgt gggtgttggt
    12181 cttgctttcc ttcaggtcaa cattatcgcg accgcattgg ccattggttg tgcaaccttg
    12241 attatgtcga cattagggat gatggttggt cgctttatcg gctcaattat tgggaaaaaa
    12301 gcggaaattc tcggcgggct ggtgctgatc ggcatcggcg tccagatcct ctggacgcac
    12361 ttccacggtt aataagaacg ctgccagagg tgaatattaa agtccgtctg gcagtcgaac
    12421 acttcttttg ctgccagtat ttgccagact tctggcttcg cacgccaggc aaacggcgtc
    12481 atctgcaata atgcgacggc ttcatcaccg cgaagacgca tcggataaca caactcatca
    12541 ctcttctgta atgtaaaacc ttccagttgt tctgcatgag gtgcatgaag atgtacttca
    12601 ttgtaaatca gccccttcag ctccatcaaa tgtcgcggtc ccggcgtggc agtaatgacc
    12661 cagccgccgg gcttcactac tcgtgctaat tcttctgctt tacacggcgc gtaaatacgt
    12721 attatggcgt ccatactggt atcggaaaac ggcaaacggt ggctggaagc gacacaaaga
    12781 gtgacctgcg gatagcgttt cgccgccgct tttatcgcta ccttcgaaac atccagacca
    12841 aacgtggtga tttcgggcaa cgcatcggca aatgcgtgtg tgtaataccc ttcaccacag
    12901 ccaatatcca gcaccgccgt ggccttttca ttaagccgtt cccgcagttg ggcgacaatt
    12961 gcatcacgca gcggctgata atgtccggca tctaagaatg cgcggcgtgc ttgcatcatt
    13021 tccgcgctgt cgcccggatc acgagaccgt ttatgctgaa cgggcagcag attgacatac
    13081 ccttctttcg ccatatcaaa ctgatgtcgt tggggacaga tatagctgtt tttttcacgc
    13141 gtaagaggct gatggcaaag tggacaggaa aacgacatga caactccggc aggatactaa
    13201 aggccgcaag tgtaacgcga atcacgcggc gggcaaatat ccgaacgcca ggagcgaaga
    13261 taaagcatag taaaaagcct cgcattcgac gaggctttat agcgagattg aagcgtattc
    13321 acacttcaga tcagtggatt cgatcagata gctgttacgt taacagctgc cggacctttc
    13381 tggccgtcct gaatttcgaa ctcaacgttc tgaccttcag ccagagtttt gaagccatta
    13441 ccctggatag cggagaagtg tacgaacacg tctttgctgc catcagcagg agtaatgaag
    13501 ccaaaacctt tagactcgtt gaaccactta acctgacctt taatctttgc catttgaaaa
    13561 attccttaga ttgttttctt cgcccgcagg cataacatag ataaaactga cacattactg
    13621 catgaggcac caatataagg ctcggcagag aagcggtatt caacgtcaac gtgtttactc
    13681 aggacttctt tactgaaaat gccacacata aacagaactg tacctcgttt aacccgaaat
    13741 ctgttatcac atacaacgtt aattatggca agccattttt aaacatgtct cgatcagacg
    13801 cacaaatctc gatacgtctt ccactttttt tgcacactta tgcaacggaa tacgcgccga
    13861 tatatcattg tgcttaacct tgtcagttcc gcagatactt aaactgacgt agtttctaac
    13921 atagttcaat aagttcagtt gttccaggtc agtgggagaa gttattacat agtgcgtgca
    13981 atatcacatt ttctgctatg caatgaataa aaagttatat cactttttct cataaaacag
    14041 gcagttaacg gctctcaatc accccaaaga gaagtcaatc ccaaaggaat tggaaattta
    14101 aaataagaaa a
//
LOCUS       DAZFAB010000114        13981 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZFAB010000114 DAZFAB010000000
VERSION     DAZFAB010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13981
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.114"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            121..1413
                     /gene="espX5"
                     /locus_tag="ACHJWA_003900"
     CDS             121..1413
                     /gene="espX5"
                     /locus_tag="ACHJWA_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector pentapeptide repeat protein EspX5"
                     /protein_id="HFO4310411.1"
                     /translation="MRYNGLNNMFFPLCLINDNHSVTSLSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKMDDHEKVIDLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIASYANNK
                     CDDRITRRVYLNKDKEVSIKVVYFINNVTVHNNTIEIPQTVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFNFAIMEKILFDNCILDDSYFAQIKMTDGT
                     LNSCSAMHVQFYNATMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYINLFRADLSF
                     SKLDLINFKHADLSRVNLNKAILQNINLIDSKLFFTRLTNTFLEMVICTDSNIANVNF
                     NNANLNNCHFNCSVLTKAWMFNTRLYRVNFDEASVQGMGISILRGEENIPINSDTLVT
                     LQKFFEEDCTSHTGMSQTENNTHEVAMKITADIMQHAD"
     gene            complement(1567..3216)
                     /locus_tag="ACHJWA_003901"
     CDS             complement(1567..3216)
                     /locus_tag="ACHJWA_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFO4310412.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(3367..4716)
                     /gene="ghxP"
                     /locus_tag="ACHJWA_003902"
                     /gene_synonym="yjcD"
     CDS             complement(3367..4716)
                     /gene="ghxP"
                     /locus_tag="ACHJWA_003902"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFO4310413.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(5262..5726)
                     /gene="soxR"
                     /locus_tag="ACHJWA_003903"
     CDS             complement(5262..5726)
                     /gene="soxR"
                     /locus_tag="ACHJWA_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFO4310414.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            5812..6135
                     /gene="soxS"
                     /locus_tag="ACHJWA_003904"
     CDS             5812..6135
                     /gene="soxS"
                     /locus_tag="ACHJWA_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFO4310415.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(6138..7724)
                     /gene="pdeC"
                     /locus_tag="ACHJWA_003905"
     CDS             complement(6138..7724)
                     /gene="pdeC"
                     /locus_tag="ACHJWA_003905"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFO4310416.1"
                     /translation="MNHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIVPVNGYTIVSADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVPRNGYLYATVY
                     STKRPIAAIVATSYQRLIAHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWPGEQGQVMNPAEFIPLAEKEGMIEQV
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            8153..8434
                     /locus_tag="ACHJWA_003906"
     CDS             8153..8434
                     /locus_tag="ACHJWA_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFO4310417.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            complement(8533..9069)
                     /gene="ssb1"
                     /locus_tag="ACHJWA_003907"
     CDS             complement(8533..9069)
                     /gene="ssb1"
                     /locus_tag="ACHJWA_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFO4310418.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            9324..12146
                     /gene="uvrA"
                     /locus_tag="ACHJWA_003908"
     CDS             9324..12146
                     /gene="uvrA"
                     /locus_tag="ACHJWA_003908"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFO4310419.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLYARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(12181..12537)
                     /locus_tag="ACHJWA_003909"
     CDS             complement(12181..12537)
                     /locus_tag="ACHJWA_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO4310420.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(12541..12957)
                     /locus_tag="ACHJWA_003910"
     CDS             complement(12541..12957)
                     /locus_tag="ACHJWA_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFO4310421.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(13068..13781)
                     /gene="aphA"
                     /locus_tag="ACHJWA_003911"
     CDS             complement(13068..13781)
                     /gene="aphA"
                     /locus_tag="ACHJWA_003911"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFO4310422.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKNFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     SMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         3738 a         3568 c         3341 g         3334 t
ORIGIN      
        1 ttttcccata atattaaaca aataaactca ttaaaaaaaa atgagcgatt tttgacagtc
       61 gtagaaaatg ataatgcaga gaatatgcct tttctttctt gttaattata aggatatttt
      121 atgcgctata atggtttaaa taatatgttt ttccctcttt gcctgattaa cgataaccac
      181 tctgtcacaa gtctatcaca tacaaagaaa acaaaatcag ataattacag caaacatcat
      241 aaaaacacgt taattgacaa taaagccctc tctcttttca aaatggatga tcatgaaaaa
      301 gtgatagact tgattcagaa aatgaaaaga atttatgata gtttaccatc aggaaaaatc
      361 acgaaagaaa cggacaggaa aatacataaa tattttatag atatagcttc atatgcaaat
      421 aataaatgtg acgatagaat tacgagaaga gtttacctta ataaagataa ggaagtgtca
      481 attaaggtgg tatattttat aaataatgtc accgtccata ataatactat cgaaatccca
      541 cagacagtga atggtggtta cgatttttca caccttagcc tgaaaggtat cgtgattaaa
      601 gatgaagatt tatccaattc gaattttgca ggttgcagac tacaaaacgc tattttccag
      661 gactgtaata tgtataaaac gaattttaat ttcgccataa tggaaaaaat actttttgat
      721 aattgtattc tcgatgactc atatttcgct cagataaaaa tgactgacgg aactctaaat
      781 tcatgctccg ctatgcatgt tcaattctac aatgcaacaa tgaatagagc caatattaaa
      841 aataccttcc ttgattattc aaatttttat atggcataca tggctgaggt aaatctttat
      901 aaagtaatag cgccatatat taatttattt agagccgacc ttagcttctc taaacttgat
      961 ttaattaact ttaaacatgc tgatctgtct cgcgtcaatc tgaacaaagc aatcctccag
     1021 aatataaact taattgatag taaactcttt tttacacggc taacaaatac gttcctcgaa
     1081 atggttatat gcaccgattc taatatagct aatgttaatt ttaataatgc caatttaaac
     1141 aattgccatt tcaactgttc tgttttaaca aaagcctgga tgtttaatac ccgtctctat
     1201 cgggttaatt tcgatgaggc tagcgtccag ggaatgggca tttccattct ccgtggggag
     1261 gagaatattc ccattaatag tgataccctg gtaacactac agaaattctt tgaagaagat
     1321 tgtacctctc atactggcat gtcacaaact gagaataata ctcatgaagt agctatgaag
     1381 attactgcag atattatgca acacgcagat tgagattaag tcgaagaaca gccaatgaaa
     1441 tgactttaaa agagagataa cttatttgtt ttagaatcat gaatgttagc ccgagctaac
     1501 ctgttaaagg tgcaaaagat tgcacctttt tcagattacc tcgggcctgg ttacgtcgct
     1561 ttcggcttac tgattttcct caattagcaa cgcttcaagc aaatcgagat cgtgcagtaa
     1621 tttttgcagc gtttcgttgc tgatctcccg cgtggcgcgc aggtggtaca gttcagcacg
     1681 ttcagaacgc aatgccgcca gacggaagcg acgctcaagg ttctcttcct gcacggaact
     1741 ttcaacgtcg ttacgtccat cggcgcgacg acgcaggtta ccaatgacgc gagaactgac
     1801 ctccgtaagc agctggttat cgatattctc ttcggtatcc gccgccagac gctcctccat
     1861 tttctggatc gccacaatcg ccacttctgc cgttgccgct cgcgcaatac gttcttcttt
     1921 caattgttgc gaatggtccg cgacttcaat gtgttgtagc agaataggca acatcaccac
     1981 gccgacaaac agcgagaaga gaatgacacc agccgccaga aacaccagct catagcgcgc
     2041 cgggaagacg ttaccatccg gcaagagcag cgggatagag agcacaccgg ccaaagtgat
     2101 cgccccacgc accccggcga aagacgcgat caggatttct cgtgtggtcc acgaaccaaa
     2161 ctccatcggc ttcttcttca ggaagcggtt gctgaacttt ttcatcgtcc acagccagcc
     2221 gaaacggacc agcatcagcg ccgcatatat cagaataata tcggtaaaca gcatccagat
     2281 ttcgacgtta gggtcgattt ctgctgccat cagcgacgtc tccagaatac ccggcagctg
     2341 cagacctaac agcaggaaca ccatgccgtt aaagacaaat tccagcatcg cccaggtgct
     2401 gtttgcacgc aggcgcattg ccagcggcgc acggcgcatc acaccggagc gggtgatggt
     2461 catcccggcg gcaaccgcag cgaggatgcc ggaaacgcca atatgttcgg caatcagata
     2521 agaagcgaat ggcagcagga acagcaggac gatctgcgtc gcgggttcat caccgcccca
     2581 gcggctgagg aatcgcagcg agcgaccgta cagccagctc accacaaaac cggcgagaat
     2641 accgccaatg gctactttca taaattcgac cgtcgcaccg ccgacggtga agatcatcgt
     2701 ccccatcgcc actgccacgg caaacttcaa cgacaccagg ccggaggcgt cattcatcaa
     2761 cgcttcgccc tgcaaaatgc ccatgatttt tttcgggatg cgcccttcgc caacaatccc
     2821 ggagagcgcc acagcatcgg tcggagaaag caccgccgcc agcgcaaagg cggggatcag
     2881 cggaatgccc ggcaccaccc agtaaataag gaagccaatg ccgaccacgg tgaccaccac
     2941 cagcgccagc gcgaggccga aaatctctcg accatgttcg agaaattcac gggtcggcgt
     3001 tttccagcca tcagcgaaca gcaacggcgg gataaacaag actaaaaaga gttcaggatc
     3061 aaactccaca tgcaaaccaa acgtcggcca cgccagtagc gcaccgatgg cgatttgcat
     3121 aagcggaagc gggatctgaa agggcatgac acgagtgacc accccggaca gcgagaccac
     3181 gagggtcatt atcagtatgg tgaagaagat ttccatgcgt tccctgtttg cgatgttttt
     3241 tatgatctga gaaagacgtt atgcctgaat tctcattttt tcagaatatc gcaaaagctc
     3301 acacaatgtg cggagaaaat aaaaacgggc agccattgcc acccgttttc agaatccgat
     3361 gaaagattag atagcccagc cacccgcata gaaggtcacc agcgccacgg cgataacgac
     3421 cgtaccgatg ttcaacttgc gccattcacc ggaaaccaga cgaccaatca ccagagtcgc
     3481 gaagccgatc atgatgcctg ttacgatgtt acaggtcagc acgatgaata ccgccgtaac
     3541 cagacccgcc atcgcatcaa caaaatcagc aaagtcgatt ttcgccacgt tgctcagcat
     3601 cagcaggcca acgtacatca gcgccggagc cgttgcatac cccggaacga ggtaagaaag
     3661 cggagagagg aacagaatca gcaggaacag cacgccaacg gtgatagccg tcaaaccggt
     3721 tttaccgccc gccgccgtac ccgccgcaga ctcgatgtat accgctgccg gagctgcacc
     3781 caccaggcca gagaaaacgc tgctcatgga gtcagtggtc agtgctttcc cgccgtcgat
     3841 gatctgccca tctttatcca gcaggttcgc ctggccggcg acagcgcgga tagttccggt
     3901 ggcatcaaat actgccgtca tcaccagcgc cagaacgctt ggcaggacta caggattcag
     3961 cgcgcccata atgtccaggc tgccaatcag tgaattgccg ttttcatcgc tcaatgaagg
     4021 catggcgaaa acgccggaga aatggacgtt aggatcgaag atcaaaccga caattgagat
     4081 accgataatg gtcagcagaa tgccaccagg gactttcagt ttttccaggc cgatgatcac
     4141 cgccagacct accagtgaca taatcaccgg gaaggtcgcg aaatcaccca gcgcaacggg
     4201 cagaccatca agcgggtttt taatcaccag accgacaccg ttagcggcaa tgagcagcag
     4261 gaacagaccg ataccgatcc ccgtgccgtg cgccacaccg tgcggcaagt tgcgcaaaat
     4321 ccagctacgg atacccgtgg cagaaattac cgtaaacagc acacccatca ggaacacggc
     4381 acccagcgcg acaggtacgc taatatgttg ccccagcacc aggctgaatg cggtaaacgc
     4441 cgtcagggag atggcgcaac caatcgccaa cggcagatta gcccacagac ccatcacgat
     4501 agaaccgagt ccggcaacca gacaggtcgc aacgaaaact gccgcaggcg ggaagcccgc
     4561 tttacccaac atgcctggaa cgacgataac cgagtagacc atcgccagaa acgttgttaa
     4621 cccggcaacc acttcctgac ggacagtgct tccacgttgt gaaattttaa accaggcgtc
     4681 gagtgaaccg ccggtacgcg ctgatggcgt agacataaga gaacatcccc tgagagttta
     4741 attttcgtca agatgcgtgg cggacaatcc cctgccagcg aaacgttatc ttcctgctcc
     4801 aggtttgcga caatttgctg cgtcgcaaaa aagcaaacgt ttagctccgc gcatacaaaa
     4861 cgggtggtaa aaaaagcaaa cgattatccg gcgatcatag cggctttttc aaccgaagtt
     4921 tgctgctttt ttctctttta ttgtcttgac gatgaaaaac caggcaattg atgcgcaaac
     4981 gttatcgtcg atgcgcaaga ttgcagcgtt atcgagccgt ataccgttga ggattgcgat
     5041 aaaaaatcgt ctgagatgac aaaaatattg cggctaaaaa gtatactttt acactggcga
     5101 agaaacgtcg gtgacataca attaaagcat caacaccaac cggaacctcc accacgtgct
     5161 cgaatgaggt gtgttgacgt cgggggaaac cctcctgtgt accagcgaga tagagagaaa
     5221 gacaaagacc ggaaaacaaa ctaaagcgcc cttgtggcgc tttagttttg ttcatcttcc
     5281 agcaagcgtg cgccggtacc ttcttctcct aagcggtcgc ccgggttacg caacgggcaa
     5341 tcactgcgcg aaaggcagcc acaaccaata catccgtcca gttcgtcacg cagcgccact
     5401 aaggtatgaa tgcgccgatc caactcttct cgccattggg acgaaagctg tttccactct
     5461 ttcgcactta acgtatgccc ttcgggcaac acgccaaacg cttcaccaat ggtcgcgaga
     5521 ggaatgccaa tacgctgagc aattttgata attgcgacat atcgcaacac atcacgttta
     5581 taacgccgct gattgccgct gttacggata ctggtaatca accctttgct ttcatagaaa
     5641 tgcagcgccg ataccgccac accgctgcgt ttcgccactt cgccgggggt tagcagcgct
     5701 ttaatgcggg gtaatttctt ttccataaat cgctttacct caagttaact tgaggaatta
     5761 tactccccaa cagatgaatt aacgaactga acactgaaaa gaggcagatt tatgtcccat
     5821 cagaaaatta ttcaggatct tatcgcatgg attgacgagc atattgacca gccgcttaac
     5881 attgatgtcg tcgcaaaaaa atcaggctat tcaaagtggt acctgcaacg gatgttccgc
     5941 acggtgacgc atcagacgct tggcgattac attcgtcaac gccgtctgtt actggccgcc
     6001 gttgagttgc gcaccaccga gcgtccgatt tttgatatcg caatggacct gggttatgtc
     6061 tctcagcaga ccttctcccg cgttttccgt cggcagtttg atcgcactcc cagcgattat
     6121 cgccaccgcc tgtagtttta ttgcccgcgc gttaactccc gcgcgggtaa ttgctccatc
     6181 cattgcataa acacctgcgg cggcatcgcc ttcgcaaaga accatccctg gcaatagcgc
     6241 acgccgcgtt tgcgcagcca gttaacctgc tcctcagttt cgacgccttc agcgatcgtt
     6301 tttaacccca ggctgtgcgc cagctcgatg atatgttcag caatcaaatg actggtttta
     6361 tgggtggtca gcgtctcaac aaacgatttg tcgattttca aaatatcgac attcaatgat
     6421 ttaaggttat gcaagttaga gtagccaata ccaaaatcat caattgccac ttcgtaacca
     6481 gcctggcgga aagccagaat aatcggcgtc attttgtcga catcaagaaa tgcatgctca
     6541 gtcacttcaa atttaatttg ctgcggacgc accgcgtatt gctctgtttt ctgattgatt
     6601 cgcgctatca accgtgacgt atgaaaatcg gaggccgaca gattaataga aacatagcga
     6661 tcagcatgtg ttgcaaggta cgcgcccaga tcgcggaaga cattatcaat aacataatca
     6721 gttacctgtt ctatcatccc ctccttttct gccagaggaa taaactctgc cggattcatt
     6781 acttgcccct gctcaccagg ccaacgtaac aacgcttcag cgccgataca tttttctgtt
     6841 ttgatatcaa ttattggctg gtaataaagg catagctgat gtttttcgag ggcgcgttgc
     6901 aatttgcgtt tgggagacaa atagttttgt cgaatacgta gccagagtaa tagcagaaca
     6961 agactcccca aaataccggc gggcaacgca aaaataagat gattataaaa atgggctata
     7021 agacgttgat atgaagtcgc aacaatggct gcaattgggc gttttgtcga ataaactgtc
     7081 gcatataaat agccatttct tggtacggtt aaatccttca aatgaatcag cggagaaaac
     7141 gttgctgctg aggcctcttt gcttaacgag aaaaaggttt tcgtcaccgt atcatacaca
     7201 ccccattgca atgtcgggtc atcagacatc acttcactcc agaagagagg gttgataacc
     7261 gccacataat ttccccgctg catataggtc attttatagc cagagaaaaa aggcgtatcg
     7321 cggtaataat agatagaaac attaggttca cgcttataat cggccgatac aatcgtatag
     7381 ccgtttacag gcactatcag cgatgagcat aaaaaatgat tatcacgggc atagatcaat
     7441 tcattaatat aaagatagcc acgaataata ttcaacattc gcttttgatg ggctggagtg
     7501 caaacttgcc cctgatagcg ttcagctgca tcgctcacta aatctgcctg gcgaatgacc
     7561 agctcggatt tatccaacgc cagttgagca aaggttcgga gctgattatt cacttctgat
     7621 tttgcccaaa ggaaggcaat ccatagcgaa agaatgatgg gaaagagaac taaaaagata
     7681 atgcccggca acgccagtaa ttggtgtcgt gcacgatgat tcattgctat caatttcctg
     7741 cctgtttata cccggccatt gcaaggtgaa accctggcta ccgacgtctt tagataaatc
     7801 ttacggtata aatagcactt ttgcctgatt tgaaattgat acctttttaa tcgtcacttg
     7861 gatgaaaata attcattgat attcaaaatt aattttctac aggaatacag aggcggcggg
     7921 aagatatacc cttaatgcca ctgaaaaacc acataatctg tacagcaata aaagtcacga
     7981 cctaatctgg ctaacaaaca atcaattgtg atatagttca caaaattaat gaaacaaaca
     8041 gagtgtttca tttttgtgtt ctgcaagccc cgcgatagcc agagtgtacc acgcctcccg
     8101 tgaacaacgc cgcgctgtcc agggcatcgg ctcttttcag aggaattgtt tgatggctac
     8161 cctcaccact ggcgtggttc ttcttcgctg gcaacttctt agtgccgtaa tgatgtttct
     8221 ggccagcacg ctcaacatcc gttttcgtcg gtctgattat gtcgggcttg cagtgatcag
     8281 cagcggtctg ggcgtggttt ccgcctgctg gttcgcaatg gggttgcttg gcatcacaat
     8341 ggcggacatc accgccatct ggcacaacat cgagtcggtg atgatagaag agatgaatca
     8401 gacaccgcca cagtggccaa tgattttgac ctgatacgta gaagcctcca taacggaggc
     8461 ttctttttta tggctggcag atgctttaat catccacctt aaaacaatat aacctattgt
     8521 tttaatgaca aatcagaacg gaatatcgtc atcaaagtcc atcggcggct cgttagacgg
     8581 cgctgccgga gcggactgct gcgggcgaga ctgcgcgccg ccgctgaact gattgccacc
     8641 ctgcggctgc tgaggctgac cccaaccgcc ctgcggctga ccaccaccga tattgccacc
     8701 tgcaggagcg ccaccaccct gacgaccacc cagcatctgc atggtgccgc caacgttcac
     8761 cacgacttct gtggtgtagc gatcctgacc ggattgatcg gtccatttac gggtacgcag
     8821 ctgaccttcg atataaacct gagaaccttt acgcagatat tcgctggcca cttctgccag
     8881 tttgccgaac agcacaacgc ggtgccattc agtctgctct ttcatctcgc cggtcgcttt
     8941 atcacgccag gattcggaag tagccagcgt aatgttggca actgcgccac catttggcat
     9001 gtagcgtact tccgggtcct gacccagatt accaacgaga ataaccttgt ttacgcctct
     9061 gctggccatg ttcgtgtctc ctgaaaaaaa atcgttctga ataagtgtaa acgcgcgatt
     9121 gtaccattac caatagcgct tttactatgt tgtgacctcg gttccgggaa acaaacctgg
     9181 ccagacattg ttacacaaca ctccgggtaa tgcattccaa tactgtatat tcattcaggt
     9241 caatttgtgt cataattaac cgtttgtgat cgccggtagc accatgccac cgggcaaaaa
     9301 agcgtttaat ccgggaaagg tgaatggata agatcgaagt tcggggcgcc cgcacccata
     9361 atctcaaaaa catcaacctc gttatccccc gcgacaagct cattgtcgtg accgggcttt
     9421 cgggttctgg caaatcctcg ctcgctttcg acaccttata tgccgaaggg cagcgccgtt
     9481 acgttgaatc cctttccgcc tacgcgcggc agtttctgtc actgatggaa aagccggacg
     9541 tcgatcatat tgaggggctt tctcctgcca tctcaattga gcagaaatcg acgtctcata
     9601 acccgcgttc tacggtgggg acaatcaccg aaatccacga ctatttgcgt ttgttgtacg
     9661 cccgcgtcgg cgaaccgcgc tgtccggacc acgacgtccc gctggcggcg caaaccgtca
     9721 gccagatggt ggataacgtg ctgtcgcagc cggaaggcaa gcgtctgatg ctgctcgcgc
     9781 caatcattaa agagcgcaaa ggcgaacaca ccaaaacgct ggagaacctg gcaagccagg
     9841 gttacatccg tgctcgtatt gatggcgaag tctgcgatct ttccgatccg ccgaaactgg
     9901 aactgcaaaa gaaacacact attgaagtgg tggttgatcg cttcaaggtg cgtgacgatc
     9961 ttacccaacg tcttgccgag tcgtttgaaa ccgcgctgga gctttccggt ggtaccgcgg
    10021 tagtggcgga tatggacgac ccgaaagcgg aagagctgct gttttccgcc aacttcgcct
    10081 gcccaatttg cggctacagt atgcgtgaac tggagccgcg actgttttcg tttaacaacc
    10141 cggcaggtgc ctgcccgacc tgcgacggcc ttggcgtaca gcaatatttc gatcctgacc
    10201 gcgtgatcca gaatccggaa ctgtcgctgg caggtggtgc gatccgtggc tgggatcgcc
    10261 gcaacttcta ttatttccag atgctgaaat cgctggcaga tcactataag ttcgacgtcg
    10321 aagcgccgtg gggcagcctg agcgcgaacg tgcataaagt ggtgttgtac ggttctggca
    10381 aagaaaacat tgaattcaaa tacatgaacg atcgtggcga tacctccatc cgtcgtcatc
    10441 cgttcgaagg cgtgctgcac aatatggagc gccgttataa agagacagaa tccagtgcgg
    10501 tacgtgaaga attagccaag ttcatcagca atcgcccgtg cgccagctgc gaagggacgc
    10561 gtctgcgtcg ggaagcgcgc cacgtgtatg tcgagaatac gccgctgccc gccatctccg
    10621 acatgagcat tggtcatgcg atggaattct tcaacaatct caaactcgca ggtcagcggg
    10681 cgaagattgc ggaaaaaatt cttaaagaga tcggcgatcg tttgaaattc ctcgttaacg
    10741 tcggcctgaa ttacctgacg ctttcccgct cggcagaaac gctttccggc ggtgaagccc
    10801 agcgtatccg tctggcgagc cagattggtg cgggcctggt tggcgttatg tacgtgctgg
    10861 atgagccgtc tatcggcctg caccagcgcg ataacgagcg cctgttgggt acgcttatcc
    10921 atctgcgcga tctcggtaat accgtgattg tggtggagca cgacgaagac gcgattcgcg
    10981 ccgctgatca tgtgatcgat atcggtcctg gtgcaggtgt acatggcggt gaagtggtcg
    11041 cggaaggtcc gctggaagcg attatggcgg tgccggagtc gttgaccgga cagtacatga
    11101 gcggtaaacg caagattgaa gtgccgaaga aacgcgttcc ggcgaatccg gaaaaagtac
    11161 ttaagctgac tggcgcacgc ggcaacaacc tgaaagacgt gacgctaacg ctgccagtcg
    11221 gtctgtttac ctgcatcaca ggggtttcag gttccggtaa atcgacgctg attaacgaca
    11281 cactgttccc gattgcccaa cgccagttga atggtgcgac catcgccgaa ccggcaccgt
    11341 atcgcgatat tcagggactg gagcatttcg acaaagtaat cgatatcgac caaagcccaa
    11401 ttggtcgtac tccgcgttct aacccggcga cctataccgg cgtgtttacg cctgtgcgcg
    11461 aactgtttgc gggcgtaccg gaatcccgtg cgcgtggtta tacgccagga cgtttcagct
    11521 ttaacgtccg tggcggacgc tgcgaggcct gtcagggcga tggcgtgatc aaagtggaga
    11581 tgcacttcct gccggacatt tacgtgccgt gcgaccagtg caaaggtaaa cgctataacc
    11641 gtgaaacgct ggagattaag tacaaaggca aaaccatcca cgaagtgctg gatatgacca
    11701 tcgaagaggc gcgtgagttc tttgatgcgg tgccagctct ggcgcgtaag ctgcaaacgt
    11761 tgatggacgt tggtctgacg tacattcgct tggggcagtc cgcaaccaca ctttctggtg
    11821 gtgaagccca gcgcgtgaag ctggcgcgtg agctgtcaaa acgcggcacc gggcagacgc
    11881 tgtatattct cgacgagccg accaccggtc tgcacttcgc cgatattcag caactgctcg
    11941 acgtactgca taaactgcgc gatcagggca acaccattgt ggtgattgag cacaatctcg
    12001 acgtgattaa aaccgctgac tggattgtcg acctggggcc ggaaggcggc agtggcggcg
    12061 gcgagatcct cgtctccggt acgccagaaa ccgtcgcgga gtgcgaagct tcgcatacgg
    12121 cacgcttcct caagccgatg ctgtaatcgt taaggccgcc ttccgagcgg ccttttcctt
    12181 tcagagttgc accagcaatt tacgtttttc ttccggcagt aaattcaccg cctgctgata
    12241 agacgcatcc accagataat agatttgcga atccggcagc gaaccgtcga gatacacggt
    12301 gctccagtgc gctttattca gatggcggct tggacgcaca tcgctgtgct gctgacgtag
    12361 cagctccgcc agctccgggc tggttttcag cgaaacagct gggcgatttt ctacttcttt
    12421 caccatcgca aacagtacat cttccacttt gatctgcgtc gctttccagt cgttatgcac
    12481 gctctgttct gcgcctggtt ttgccatgca atattgtagc aactccgaaa tggtcatttt
    12541 ttactcccct tgtagtgtcg cgatgatgcg acgcgatccg ccgtggatgc gatgttcccc
    12601 cagccaaatg ccttgccagg tgccggtctg aatacgccct ttatgcaccg gcaatacaag
    12661 cgatgttccc agcattgagg atttgatatg agaaggcata tcgtctgctc cctcatagtc
    12721 atgctcataa tttccgttgt cgggaacggt gcggaggaaa aaacgctcca tgtcgtggcg
    12781 tacggtggga tcgcagttct cattaagtgt cagagaggcg gaggtatgtt gcagcaacag
    12841 atgcagtaag ccgatgttaa cgcgcggcat atcagccagc tgattcagaa tttcatccgt
    12901 taccagatga aacccacgag atttggcgct aagcgtgagc gtcttttgat accacatgtg
    12961 ctgctccttg ataaaactct cttaatcagt ttgcagcaag acagcgaaag gataaaggtg
    13021 tgattaaaaa aacagcattc gggagagtgt cacgctctcc cgctctgtca gtattctgaa
    13081 ttgacgatca cctcttcacc aaacgcaccc gcttgtggca agggtttgta ggtagagttg
    13141 gaggcacgca gaatgcggat accacgagcg ccgacatcgc gtgcggcggt aatatcgtta
    13201 tcggaatcgc cataaaaaat tcggatattt ttatcctgca gccattgcga ttttgtattt
    13261 tgccctggtt tatcgcccgc aaagatcacc ggattcatgc tggtggcagg aatatgaaaa
    13321 ttatccgcca gcgtttttga aaccgtttct gttttcgtcg ggctacgacc agtcacaaag
    13381 aagatcgcgt caccacggcg tacatgcata tcaatcagct ggcgagcgac ctcttttgga
    13441 atgctgaatt catcccagcc gttgttcatt ttttcccaga atacagggtt tttcagataa
    13501 tcttcgcttt ctggcgagaa gttttttttg ccgcgccaga agcccggact ggaaaaaagt
    13561 accgtgtcat cgatatcaaa ccccaccgcc attggcggac gccctgcgag gctattttca
    13621 atttgtgcga ccgaaaccca atgaattggt gcctgttcag caagcctggc aacgttagtc
    13681 ccagggttaa gcggtgaagg agatgaggcc agggcaacag cggaactgtt tagagcgaac
    13741 aataagcaaa cggcactgat tgcctgtgtg atcttgcgca tatttttccc taaatagtca
    13801 gtatgttgaa actttttatt gtgagatttg ttgcaaaaac tatctgacca taacgccagc
    13861 tacaggggaa aggaaggatt atctgcggtt tttgtgatga aaagcaggat gattatattt
    13921 aacctagtaa atacgttcct gtcgtttttt tgtaaaaaat ggttaatgct cgatgtaagg
    13981 a
//
LOCUS       DAZFAB010000115        13842 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZFAB010000115 DAZFAB010000000
VERSION     DAZFAB010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13842
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.115"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            5..258
                     /gene="yahH"
                     /locus_tag="ACHJWA_003912"
                     /pseudo
     CDS             5..258
                     /gene="yahH"
                     /locus_tag="ACHJWA_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahH"
     gene            308..1258
                     /gene="yahI"
                     /locus_tag="ACHJWA_003913"
     CDS             308..1258
                     /gene="yahI"
                     /locus_tag="ACHJWA_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFO4310423.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDELQKANPDWRFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTDAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPEKQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            1268..2650
                     /gene="yahJ"
                     /locus_tag="ACHJWA_003914"
     CDS             1268..2650
                     /gene="yahJ"
                     /locus_tag="ACHJWA_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFO4310424.1"
                     /translation="MKENNSRREFLSQSGKMVTAAALFGTSVPLAHAAVSGTTNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQNGKIVALRENKQHPDAT
                     LPHYDAGDKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDELAHRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            2949..3359
                     /locus_tag="ACHJWA_003915"
     CDS             2949..3359
                     /locus_tag="ACHJWA_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFO4310425.1"
                     /translation="MTIKEKFSQKYPHASFCTFGDSAALADHLATLIATGVKTASCGS
                     LAGCIEDNAFPMIGEYKIVENSRGEPVCVIRVIGLHLLRFSDVTAELARKEGEGDLSL
                     EYWRNEHRRFFQAEGSYSPEMDVIFEEYALIDVV"
     gene            3613..4596
                     /locus_tag="ACHJWA_003916"
     CDS             3613..4596
                     /locus_tag="ACHJWA_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO4310426.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVEAGKAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDVSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDPASAGYAL
                     AAVASTLLKGEEIKPGLEMQNLGKADVDMDKRIIRFHKVLLVNKDNIDSLY"
     gene            4645..6129
                     /locus_tag="ACHJWA_003917"
     CDS             4645..6129
                     /locus_tag="ACHJWA_003917"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO4310427.1"
                     /translation="METFLSLRHINKTFHATRALRDVSLDFMSGEVHCLAGQNGCGKS
                     TLIKIMSGVYRPDEGAEITLGGKNWSKLTPAASVAQGIQVIYQDLSLFPNLSVWENIA
                     VNHYHHGLFVNRRRLREVAQAAMTSINVTLPLDTLVSELSIARCQLVAICRALAQDAR
                     LIVMDEPTASLTHQEVQGLLQVVHQLRERGICVVFVSHRLEEVMEVSDRISVLKDGEL
                     VGTFPAAEMTTKQLGFLMTGQEFEYQVRELWQGKSSTPVLEVRNLSRHGEYLNINLRV
                     EAGEVVSIVGLLGAGRTELCLSLFGMTRPDAGEILINGQLVTLHSNQDAIRHGIGYVS
                     EDRMSRGLVMAQSIEDNIISTVFHKVKDRFGFLSETKVRDLVDRLIKALTIKVSDPHL
                     PVNTLSGGNAQRVSIAKWLAIGPRLLILDSPTVGVDIANKAGIYGIISDLAAHGIAVL
                     MICDEIEEAWYQSHRILVMQKGQITHSFLPDSSSQARIAEVVNG"
     gene            6122..7093
                     /locus_tag="ACHJWA_003918"
     CDS             6122..7093
                     /locus_tag="ACHJWA_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4310428.1"
                     /translation="MAELKKRHEFWLALLIVVLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKIGGNFAVAFALAGCIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            7090..8046
                     /locus_tag="ACHJWA_003919"
     CDS             7090..8046
                     /locus_tag="ACHJWA_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4310429.1"
                     /translation="MKKSWRNNVEFYLIGLLVLTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVLPILATLGMMTLLKGINILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFAAVALGLWILLEKTPLGKAIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQARGMLGLDRVV"
     gene            8133..9182
                     /gene="yahK"
                     /locus_tag="ACHJWA_003920"
     CDS             8133..9182
                     /gene="yahK"
                     /locus_tag="ACHJWA_003920"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFO4310430.1"
                     /translation="MKIKAVGSYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            9425..10240
                     /gene="yahL"
                     /locus_tag="ACHJWA_003921"
     CDS             9425..10240
                     /gene="yahL"
                     /locus_tag="ACHJWA_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HFO4310431.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFINSVRTDC
                     KCILLEHHMPLLRLFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYP
                     VFPESLVMLLLSVLDVKTYRDDARLNEGISNRVQELGDRYYPVNKHVKIRYTL"
     gene            10691..10900
                     /locus_tag="ACHJWA_003922"
     CDS             10691..10900
                     /locus_tag="ACHJWA_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310432.1"
                     /translation="MPLLSSLQSGTLPLFGYSGRVRPMKKAHTGESGLKWEAKDSSKL
                     IGNKDHALRGLSSPMVGIRQIRLIT"
     gene            complement(10917..11588)
                     /gene="yahN"
                     /locus_tag="ACHJWA_003923"
     CDS             complement(10917..11588)
                     /gene="yahN"
                     /locus_tag="ACHJWA_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFO4310433.1"
                     /translation="MMQLVHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPINAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASIIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            11735..12010
                     /gene="yahO"
                     /locus_tag="ACHJWA_003924"
     CDS             11735..12010
                     /gene="yahO"
                     /locus_tag="ACHJWA_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein YahO"
                     /protein_id="HFO4310434.1"
                     /translation="MKIISKMLVGALAFAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTADIYKKK"
     gene            complement(12108..13694)
                     /gene="prpR"
                     /locus_tag="ACHJWA_003925"
     CDS             complement(12108..13694)
                     /gene="prpR"
                     /locus_tag="ACHJWA_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HFO4310435.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRQGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKIPAPRLLTPQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
BASE COUNT         3470 a         3230 c         3638 g         3504 t
ORIGIN      
        1 cggcgtgaac gccttatccg gcctacgaat ggcgcaagaa tctgtaggcc tgataagcgt
       61 agcgcatcag gcatttgtca ccattgccgg atgcggcgtg aacgccttat ccggcctacg
      121 aatggcacga gaattgtagg cctgataagc gtagcgcatc aggcatttgt caccattgcc
      181 ggatgcggcg tgaacgcctt atccggccta cgaatggcac gagaatttgt aggcctgata
      241 agcgtagcgc atcaggcagt ctggcgttgg tcataacccc atcaccctct gtagcggaca
      301 taacaacatg aaagaacttg tggtcgttgc cattggtggc aacagcatta tcaaagataa
      361 cgccagccag tcgattgagc atcaggcgga ggcggtgaaa gccgtggccg atacggtgct
      421 ggaaatgctg gcttccgatt acgacattgt gctgacccac ggtaacgggc cgcaggtcgg
      481 gctggattta cgccgtgcgg agattgccca cgagcgcgaa gggctgccct taacgccgct
      541 ggcgaactgt gtggcagata cgcagggcgg cattggctac ctgatccaac aggcgctgaa
      601 caaccggctg gcgcgtcacg gcgagaaaaa agccgtcacc gtggtgactc aggtggaagt
      661 ggataaaaac gatccaggtt ttgcccatcc caccaagccc attggtgcat tcttcagtga
      721 aagccagcgt gacgaattac aaaaggcaaa ccctgactgg cgttttgttg aagatgctgg
      781 acggggctat cgccgcgtgg tcgcctcgcc ggaaccgaaa cgtattgtcg aagcacccgc
      841 cattaaggcg ctgatccaac aaggctttgt ggtgattggc gcgggcggcg gcggaattcc
      901 tgtggtgcgt actgacgcgg gagattacca aagcgtggac gcggttatcg acaaagatct
      961 ctctaccgcg ctgctggccc gtgaaattca cgccgacatt ctggtgatca ccaccggcgt
     1021 cgaaaaagtc tgtatccact ttggtaaacc cgagaagcag gcgctggatc gggtggatat
     1081 tgccaccatg actcgctata tgcaggaagg acatttcccg cccggcagca tgttgccaaa
     1141 aatcatcgcc agcctgacgt tcctggaaca gggcggtaaa gaagtgatta tcaccacgcc
     1201 ggaatgcctg ccagcggcgc tgcgcggtga aacgggcacc cacattatta aaacgtaagg
     1261 acgtaagatg aaagaaaata atagccgccg tgaatttctg agccagagcg gtaagatggt
     1321 caccgccgcc gcgctgtttg gtacctctgt gccgctcgcc catgcggcgg tctctggcac
     1381 cacaaactgc gaagcgaaca acaccatgaa aatcactgac ccgcattact atctcgacaa
     1441 cgtgctgctg gaaaccggtt ttgactacga aaatggcgtg gcggtgcaga cccgcacggc
     1501 gcgccagacc gtggagattc agaacggcaa aattgtcgcg ctgcgcgaga ataagcagca
     1561 cccggatgcc acgctgccgc actatgacgc tggcgataag ctgatgctgc ccaccacccg
     1621 cgacatgcat attcatctcg acaaaacgtt ttacggcggg ccgtggcgct cgctcaatcg
     1681 cccggcaggc accaccatcc aggacatgat caaactcgag cagaaaatgc tgccggagct
     1741 gcaaccgtac actcaggagc gggcagaaaa actgattgat ttattgcagt cgaaaggcac
     1801 caccattgcc cgcagccact gcaatatcga accggtttcc ggcctgaaaa atctgcaaaa
     1861 tttgcaggcg gtgctggcgc gacgtcaggc gggctttgag tgtgaaatcg tcgccttccc
     1921 gcagcacggt ttgctgctgt cgaaatcgga acccttaatg cgtgaagcaa tgcaggcggg
     1981 tgcgcattac gtcggcgggc tggacccgac cagtgtcgat ggcgcgatgg aaaaatccct
     2041 cgacaccatg ttccagattg cgctggacta cgacaaaggc gtcgatattc acctgcacga
     2101 aaccactccg tcgggcgtgg cagccatcaa ttatatggtt gaaacggtag agaaaacgcc
     2161 tcaactgaaa ggtaagctga ccatcagcca cgcctttgcg ctggctacgc tcaacgagca
     2221 acaggtagat gaactggcgc atcgcatggc ggcgcagcaa atttctatcg cctcgacggt
     2281 gccgattggc acgctgcata tgccgctcaa acagttgcac gacaaaggcg taaaagtgat
     2341 gactggcact gacagcgtta tcgaccactg gtcgccttat ggtctgggcg acatgctgga
     2401 aaaagccaat ctctacgcgc agctctatat tcgtcctaac gaacagaacc tctcccgttc
     2461 gctgtttcta gccactggcg atgtattgcc gctgaatgaa aaaggcgagc gtgtatggcc
     2521 aaaagcgcag gatgacgcca gctttgtgct ggtggacgcc tcctgttccg ccgaggcggt
     2581 ggcgcgtatc tcgccgagaa ccgcaacgtt ccataaaggg caactggtgt gggggagtgt
     2641 ggcaggttga tgcgcatttg aaacgcctgc aacggtgaat agtaagagat ttaagcccca
     2701 gggagtgggg cgggaatggt ttgctaatga atgatcctga actttataat ctcccggcat
     2761 aatttccagg ggattatttg agagaaatac acaaaagtga gttttaacaa aggtatttat
     2821 caggccataa atgtgacctg cttcggataa aaaagattca gtatcgaaaa accaatgata
     2881 ctcttgctga tggttaccaa cagtaattaa gatgtgattt gtttttccgt aatcgcaaaa
     2941 atattgccat gaccataaaa gaaaaattct cacaaaaata tccccatgct tccttttgta
     3001 cttttggcga ttcagccgcg ttggccgatc acctcgcaac gttgattgcg actggtgtta
     3061 aaacagcttc ctgtggttcg ctggcgggat gtattgaaga caacgcattt ccgatgattg
     3121 gtgagtataa gattgtagaa aatagccgtg gtgagcctgt atgtgttatc agagtgattg
     3181 gcctgcattt actgcgcttt tctgatgtca ccgcggagct tgcccgtaag gaaggtgaag
     3241 gtgacctcag tcttgaatat tggcgtaatg aacaccgtcg ttttttccag gcagaaggaa
     3301 gctattcacc agaaatggac gttatctttg aagaatatgc ccttattgac gttgtgtaga
     3361 taactattta tctacatgaa ataagctcac aaacataaaa tatcgctaat tttgtttatt
     3421 ttgctgtttt ttacgtgatg aatgtcacac ttattattgc tgcccgtttt gtccatattt
     3481 ataaccatat ttttaatagc tgcctgaaat agagctgttt attctgcata aaaatattcg
     3541 aaaattctta ttctctccag cgcgctaatt tgggcgaaca cttcctgact accctgcaat
     3601 gaggctgaaa tgatgaataa acgttttgtt atcaatatgg tgtcttcgct gttattaggc
     3661 gctgcgctaa tttctgcgcc gctccaggca gcggaaaaag tcgtggtgaa tatttctaaa
     3721 gtcgatggta tgccgtggtt taatcgcatg ggcgaaggtg tggttgaggc gggtaaagcc
     3781 tttggtgtta atgcctcaca ggtggggcct tcaagtactg atgctccgca acaagttaaa
     3841 attattgagg atttaatcgc ccgtaaggtt aatgcaatta ctatcgtgcc aaacgatgcc
     3901 aacgtgctgg aaccggtatt caaaaaagcg cgagatgccg gaattgtggt tctgaccaac
     3961 gaatcccccg gtcagccaag tgctaactgg gatatcgaaa tcattgataa cgaaaaattt
     4021 gccgctgaat atgttgagca tatggcgaaa cgcatgggtg gcaagggcgg ctatgtgatt
     4081 tacgtgggta gtttgaccgt gccgcagcat aacttatggg ctgacttgct ggtgaaatac
     4141 cagaaagagc attacccgga tatgcatgaa gttacccgcc gcatgccggt tgccgagagt
     4201 gtggatgtct cacgtcgtac tacactggac ctgatgaaaa cctatcctga cttgaaagcg
     4261 gttgtctcct ttggttcaaa cggtccgatt ggcgcgggac gtgcggtgaa agagaaacgg
     4321 gcgaaaaata aagtcgcagt ctatggcatg atgatccctt cacaggcggc atcactgatt
     4381 aaaagtggtg atatcaccga agggattact tacgatccgg cctctgcagg ttacgcactc
     4441 gcagccgtcg ccagcacgtt gctgaagggt gaagagatca agcctggatt agaaatgcag
     4501 aatctgggta aggccgacgt agatatggat aagcgaatta tccgtttcca taaggtatta
     4561 ctggtcaaca aagacaatat cgactcactt tattaatcca tttcttcggg gcgaaagccc
     4621 cgattcttat tgccagggga tgttatggaa accttccttt cccttcgtca tatcaacaaa
     4681 acgtttcatg ccacgcgagc gctgcgcgat gtttctctcg actttatgtc gggtgaagtg
     4741 cactgccttg ccgggcaaaa cggctgtggt aaatcgacgt tgattaaaat tatgtccggt
     4801 gtttatcgcc cggatgaggg agcggaaatt acgcttggtg ggaaaaactg gtcaaagctg
     4861 acacccgccg cttcggtggc gcaggggatt caggtgattt atcaggacct ctctttattt
     4921 cctaacctga gtgtctggga gaatatcgcc gtgaatcact atcaccacgg tctgtttgtt
     4981 aaccgccgtc gtctgcgtga ggtggcgcag gcggcaatga ctagtattaa cgtcacattg
     5041 ccactggata cgctcgtttc tgagctttca attgcccgct gtcagctggt ggcaatttgc
     5101 cgtgcactgg cgcaggacgc acggttgatc gtgatggatg aaccaaccgc ttcgctgacc
     5161 catcaggaag tgcaagggtt attacaggtg gtgcatcaat tgcgtgaacg cgggatctgc
     5221 gtggtctttg tcagtcatcg tctggaagaa gtgatggaag tttccgaccg tatttcagtg
     5281 ctgaaagatg gtgagctggt cgggactttt ccggctgcag agatgaccac aaaacaactc
     5341 ggtttcctga tgaccggcca ggagtttgaa tatcaggtgc gagagttgtg gcagggaaaa
     5401 tccagcaccc cggtgctgga ggtgcgtaat ttaagtcgtc atggggaata tctaaatatt
     5461 aacttgcggg tggaagcggg cgaagtggta tcgattgttg gcctgcttgg tgcggggcgc
     5521 acggaattat gtctgagtct gtttggtatg acccgaccag atgctggcga gatcctcatc
     5581 aacggccagc tggttacact gcatagtaac caggacgcca tccgccacgg tattggttat
     5641 gtttcggaag atcgcatgtc gcgcggtttg gtgatggcgc aatctattga agacaacatc
     5701 attagcactg tttttcacaa ggtaaaagat cgcttcggct ttctgagtga aactaaagtg
     5761 cgtgacctgg tggacaggct aatcaaggca ttgaccatca aagtctctga tcctcatctt
     5821 ccggtgaata cgctttctgg tggtaacgcg cagcgagtat cgatcgccaa atggctggcg
     5881 attgggccac gactgttgat tctcgatagc ccgacagtcg gtgttgatat cgccaacaaa
     5941 gcaggaatat acggcattat cagcgatctg gcggctcatg gcattgcggt attgatgatc
     6001 tgcgatgaaa ttgaagaagc gtggtatcaa agccaccgga ttttggtaat gcaaaaaggt
     6061 cagatcaccc atagcttcct gcctgacagc agttctcagg ccaggattgc ggaggtggta
     6121 aatggctgaa ctgaaaaaac gccatgaatt ctggctggca ttactgattg tggtgctgtt
     6181 tgttggcctt gcctggcgca gcgacgagtt tctgacattc ggtaatttgt acgatctcgc
     6241 caataactat gccatgttga ctattctcgc ctgtggtttg tttgtggtgt tgatttccgg
     6301 tggaattgat atttcgtttc cagcaatgac catcattgcg caatacggca tggtgctgtt
     6361 gctgcaaaaa attggtggca acttcgctgt cgcgtttgca ctggcaggct gcatcggcat
     6421 tttacttggc ttaattaacg ccttactggt taatcgccta cgggtgcctt ctatcatcat
     6481 cactatctcg acgctgaata ttttctatgg cctgctgtta tggttgagta aaggtgtgtg
     6541 gctgtacgac tttccgccgt ggtttgagca gggggttatg ttgttcaagt acaccgatgc
     6601 tgatggctat gactatggcc ttggtctgcc gctgatcgcc atgattacgg tggtgctgct
     6661 gacagcgttt atcatgaatt tcaccagtgt agggcgcaaa atttatgccc ttggcgggaa
     6721 ccgcgaatca gccagtcgca tcggctttag cgtgctgaaa ctgcaacttt tcgtctacgg
     6781 ctatatggga ttgatgtctg gcgctgcggg tgtagtgcag tcgtggacgg tgatgactgt
     6841 cgcccccgat tctcttctgg gttatgagct gacagtactg gctgcggtgg tgcttggcgg
     6901 cactagtttg ctcggcgggc gcggcacgtt aactggtact ttgctcggcg tggtgttgtt
     6961 ggcagtgatg caaaacgggc taaatttatt gggagtctcg tcttactggc aaacattgat
     7021 caccggcatc atcatcgttg ccagcattag cgccacggcg tggagtcagc atcagaaccg
     7081 gagcctgcta tgaaaaaatc gtggcgtaat aacgttgagt tttacttgat cggcctgctg
     7141 gtgctaactg ttgccgcgtt cagcattact atgccggaga ttttctggtc gataagtaat
     7201 ttccagtccg tcgcttcgca aatgcccgtg ctgggcattc tggcgttggc tatggcggtg
     7261 accatgcttt gcggtggcat taacttgtca attatagcca ctgctaacgc ctgttcactg
     7321 gtgatggcgt gggtggcgac tcagtatcca ccgggtattg ctacggttgt cgctacgtta
     7381 ctggcgggcg ctggcgcggc ggtgataatc ggtttatgta acggcgtatt gattgctggt
     7441 attcgagtgt tgccaattct tgcaacgctt gggatgatga cgttgctcaa agggataaat
     7501 attctggtga caggtggtag cgccattgcc aattatccct catgggtgct gtggttgaat
     7561 catgcgcaat ggttcggtat tccgctaccg atgtggttgt ttgccgcagt ggcactgggg
     7621 ctgtggatat tgctggagaa aacgccgctc ggtaaagcca tttacctgat tgggtctaac
     7681 gaacgagcaa cactttactc cggcattaac actcgtcggg tgctgatttg ggtttatgtc
     7741 atttccgcct tgctttgtgc ggtagcggca tttttgatga tgtcgaaact caactcggct
     7801 aaggcctctt atggcgaatc gtacttgctg gtgtcaatcc tcgcggcagt actcggcggg
     7861 gttaatccgg acggcggcag cgggcggata attggtatgg tgctggcgct gtttcttctg
     7921 caaattattg aaagcggctt caatattctc ggtataagcc cctacctgac gatggcctta
     7981 tggggaacgt tgctgctctg tttcattcag gctcgcggca tgttggggct ggatcgggtg
     8041 gtttaaatat tggctatatt caatggacgc gttttgccgc gatgacatat caggcgtcgc
     8101 caaatacaca tagctaatca ggagtaaaca caatgaagat caaagctgtt ggttcatatt
     8161 ccgctaaaca accacttgaa ccgatggata tcacccggcg tgaaccggga ccgaatgatg
     8221 tcaaaatcga aatcgcttac tgtggcgtct gccattccga tctccaccag gtccgttccg
     8281 agtgggcggg gacggtttac ccctgcgtgc cgggtcatga aattgtgggg cgtgtggtag
     8341 ccgttggtga tcaggtagaa aaacatgcgc cgggcgattt ggtcggtgtc ggctgcattg
     8401 tcgacagttg taaacattgc gaagagtgtg aagacgggct ggaaaactac tgtgatcaca
     8461 tgaccggcac ctataactcg ccgacgccgg acgaaccggg ccatactctg ggcggctact
     8521 cacaacagat cgtcgttcat gagcgatatg ttctgcgtat tcgtcacccg caagagcagc
     8581 tggcggcggt ggctcctttg ttgtgtgcag ggatcaccac gtattcgccg ctacgtcact
     8641 ggcaggccgg gccgggtaaa aaagtgggcg tggtcggcat cggcggtctg ggacatatgg
     8701 ggattaagct ggcccacgcg atgggggcac atgtggtggc atttaccact tctgaggcaa
     8761 aacgcgaagc ggcaaaagcc ctgggggccg atgaagttgt taactcacgc aatgccgatg
     8821 agatggcggc tcatctgaag agtttcgatt tcattttgaa tacagtagct gcgccacata
     8881 atctcgacga ttttaccacc ttgctgaagc gtgatggcac catgacgctg gttggtgcgc
     8941 ctgcgacacc gcataaatcg ccggaagttt tcaacctgat catgaaacgc cgtgcgatag
     9001 ccggttctat gattggcggc attccagaaa ctcaggagat gctcgatttt tgcgccgaac
     9061 atggcatcgt ggctgatata gagatgattc gggccgatca aattaatgaa gcctatgagc
     9121 gaatgctgcg tggtgatgtg aaatatcgtt ttgttatcga taatcgcaca ctaacagact
     9181 gaaaaaatta ataaatacgc tgtggtttaa catattaact tcgctctcca cttaactttt
     9241 tagttaagga gagcgaaata atatcaaagt agcagtaaaa cctataacgt aaattcaaat
     9301 tgttaaatta actccctcca gtacctaata cttcacacgt tagttatgag cgatttctga
     9361 tagtgcctgg tttaatcaga gctttattat ctgcgacgtt tatttttatt taagagagag
     9421 cgcgatgata tcattaaaag ctccgcacaa taatttgatg ccatatacgc aacaaagcat
     9481 acttaatacg gttaaaaaca atcagttacc agaggatatc aaaagctccc tggtttcctg
     9541 tgtcgatata ttcaaggttt tgattaaaca atattatgat tatccttatg attgtcgtga
     9601 tgatttagtc gatgacgaca aactcattca tctcatggct gccgtacgag actgtgagtg
     9661 gtcagacgat aacgcactca ccataaatgt gcagtttaat gatttccctg gattttatga
     9721 ctggatggat taccctgatc acccggttaa gtttgttttt cgcatacttg aaaatcaaaa
     9781 agggacagtt tgggtatatg accaggatga tgcgtttctt gatattaaag caaatgtgca
     9841 ggctggacgc tttaccgggc ttaagaaatt agtgcagttt atcaattcag tgcgaacaga
     9901 ttgcaaatgt attcttcttg aacaccatat gcctttgctg agacttttcc ctaaagggaa
     9961 agagtgtatg catgtggaaa aatggcttcg cgaaatgtcg tctattccgg agacagacgc
    10021 gcccattaag caggcgcttg cgcatggatt attattacac ttaaaaaata tctaccctgt
    10081 ttttcctgaa tcactggtga tgttattgct ctctgttctg gacgtaaaaa catacagaga
    10141 tgatgcacga ttaaatgaag ggatatctaa tagagtccag gaattaggtg acagatatta
    10201 tcctgtaaat aagcacgtta aaatccgcta cactctttag ttcagaaagg gttccacctg
    10261 caagttgtta ttctacctga gagtgaatcg caatgcaggt aatcatttca tctgtatatc
    10321 tgtatgccaa ctggcattcc atctcttttt tcgccgatat tttgttgtga tcggcgacac
    10381 ttcgcttaaa aaagcaccag tagtggtttc gcagccatgc ggtgtataaa aaaaatgatc
    10441 tcatgcagat gttttgtgaa tgtgtgggtt gacattcata tgaaaaaaat cataattcca
    10501 tcatgtttgt gtggggtctt ttctgtatct tacgcatcgc actcaagcct gacagaaaat
    10561 atgctgtaag gctcatatca aaaaccgcca ttagctcatc aggaagagca gacgacaacc
    10621 aggattgttg tatggtacgg ggttcgaggc cccgatggcg gtccaacttt tcaaaactct
    10681 tctgaattag atccccttat tatcatccct gcaaagtgga acattgccat tgtttggcta
    10741 ttcaggacgg gtcagaccaa tgaaaaaagc ccacacaggg gagagtgggc tgaaatggga
    10801 agctaaagac tcaagtaaac ttatcggaaa taaggaccac gcattacggg ggctatcatc
    10861 gccgatggtc gggattcggc aaatcaggtt gattacgtag agagtaaatt atctgctcac
    10921 cgctgcgtca ccccttcgta aatcaggcgt agcgcgaata caccaataat tgcaccaata
    10981 acccgactgg caacgcgttg catacgccca taagcacgac gcacagcggg caaagaaaac
    11041 gcctgactaa gaaaaactcg ccagataatt gatgcgagca caatccccgc ccaggccatt
    11101 aaacgtgccc atgttggtgt ttcggcattt aatgttactg agaaaatact aataaaaaat
    11161 aaaacggttt gtgggttaga gagatcggta attaatccac ggcgaaaaaa gacataccag
    11221 ggggcgttaa tcggttgttg tagtgtgctc atttgcggtg ttgactggcg gcgcatgctg
    11281 caccacgcaa accataagag ataagcgccg ccgacgattc tgataagcga aaaaatctcc
    11341 tcacactgcg taattagcgt tgcaagacca aacaaaccca acccggaata aaatgcatcg
    11401 cccagcgcca cgcccagtcc ggtcagcact cctgcgcgtc gaccggaagc caggctggtt
    11461 tgtactacca caaaaagatt ggctcccgga ttaaaaaaag taatcacgaa cagtcctacg
    11521 gtcaggtaaa cggcatgcaa aggatccata gtgatttcat ccataaataa gtgaactaac
    11581 tgcatcatat ttctaccaaa aataatcggg tgcgagagag atcacaaagt gtcttatttc
    11641 cggttactgg cgtttatgcc ctgactgaac taattattaa tcaacccaat aatgtcggtg
    11701 ggtgatagtg tgataacaac tctggagccg taatatgaaa ataatctcta aaatgttagt
    11761 cggtgcgtta gcgtttgccg ttaccaatgt ctatgccgct gaattgatga ccaaagcgga
    11821 atttgaaaaa gttgaatcgc agtatgaaaa aataggtgat atttcaacca gtaatgaaat
    11881 gtcgactgca gatgcaaaag aagatttaat caaaaaagcc gatgaaaaag gggctgatgt
    11941 gttggtactg acctccggtc aaactgacaa taagatccac ggcacggcgg atatttataa
    12001 gaagaagtaa ttctgaatcc tatgtaaaca tccccgatgc gtaagtttat cggtgatcat
    12061 ctattgaaat ttatgccgga taaagcgttc gcgctgcatt cggcagttca gcttttcagc
    12121 cgccgccaga acgtcgtccg gctgatgcct aaataattcg ccgctgctgt tttatcgcca
    12181 ttaaatttct ccagtgcctg ttgtggtgtc agcaagcgtg gagcgggaat tttcgccgac
    12241 tcgcgcgcca gttccggcag tagcagctgc aaaaactgcg gcgttaaatc cggcgtcggt
    12301 tccacactta aaaatagcgc cagtcgctcc atcatattgc gcagttcacg aatattacct
    12361 ggccagtcgt agtgcaccag cacggtttcg cttgcctgta atccctggcg taatgcggca
    12421 gaaaatgggg cggagagcgc cgccagagac actttcaaaa agctttccgc cagcggaaga
    12481 atatctgcca cccgctcgcg cagtggtggc aattgcagac gcaaaatact cagccgataa
    12541 aacagatcac ggcgaaactg cccttgccgc atatcttctt ccagattgca gtgagtggcg
    12601 ctaatgaccc gtacatctac cggaacaggc tgatgcccgc cgacgcgggt gacctctttt
    12661 tcttccagca cccgtaacag gcgagtctgc aaaggtagcg gcatttcgcc aatctcatcc
    12721 agaaacagcg tgccaccgtg ggcaatttcg aacagcccgg cgcgacctcc gcgtcgcgag
    12781 ccggtaaacg ccccttcctc atagccaaac agctccgctt ccagcagcga ttcggcaatc
    12841 gccccgcagt tgacggcaac aaacggatgc gactttttgc cctgtcgcgc atcgtggcgg
    12901 gcaaaatatt cccgatgaat tgcctgggcc gccagctctt tgcccgtccc cgtttccccc
    12961 tcaatcaaca ccgccgcact ggagcgggca tacagcaaaa tagtctgccg cacctgctcc
    13021 atctgtggtg attgaccgag catatcgccc agcacgtaac gagtacgcag ggcgttgcgg
    13081 gtggcatcgt gagtgttatg gcgtaacgac atgcgcgtca tatccagcgc atcgctgaac
    13141 gcctggcgca cggtggcggc ggaatagata aaaattccgg tcattccggc ttcttctgcc
    13201 agatcggtaa tcagccccgc gccgaccacc gcttcggtgc cgttagcttt tagctcgtta
    13261 atctgcccgc gtgcgtcttc ttcggtaatg tagctacgtt gatcgaggcg caaattaaag
    13321 gttttttgaa acgccaccag tgccggaata gtttcctgat aagtgacaac gccgatagaa
    13381 gaggtgagtt ttccggcttt tgccagtgcc tgtaacacat cgtagccgct cggtttaatc
    13441 aaaataactg gcactgacag gcggcttttc aggtacgcgc cgttagagcc agcggcgatg
    13501 atggcgtcac agcgttcgtt tgccagtttc ttgcggatgt aggtcactgc tttttcaaag
    13561 ccaagctgaa taggggtaat gttcgccagg tgatcaaact cgaggctgat atcgcgaaac
    13621 agctcgaata agcgcgttac agataccgtc cagataaccg gtttgtcgtc attcagccgt
    13681 ggtggatgtg ccatagcgca ccgcaaagtt aagataccga atattgggtt tagtcttgtt
    13741 tcataattgt tgcaatgaaa cgcggtgaaa cattgcctga aacgttaact gaaacgcata
    13801 tttgcggatt agttcatgac tttatctcta acaaattgaa at
//
LOCUS       DAZFAB010000116        13466 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZFAB010000116 DAZFAB010000000
VERSION     DAZFAB010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13466
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.116"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            142..1200
                     /locus_tag="ACHJWA_003926"
     CDS             142..1200
                     /locus_tag="ACHJWA_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HFO4310436.1"
                     /translation="MNKKIWFMEGLSSQRDIIQGVKSFAQKNNFAITVFASHRNERNE
                     ILSVADYSLTEPEDPQKRLQFIQETIQTYGIHHIHTGRNSQWFEEHRSAIEPTGATLT
                     TGATGVDWLTLADEKVTFAQFMEQKGLPVVPSWRVNTLAELKTHLAAPPFTDSPVCVK
                     PVTGIYGMGFWRFDDSASPMAVFNHPEHRLVSPQQYIAAASAAESFKPLVLMPYLPGP
                     EFSVDILADKGEILAAVGRRKEGAIQYLVNEGSAWELACDCARVMKADGLVNVQTRND
                     VNGNPVLLETNMRPSGGVGYTLHSGVNLPGLFAAFKLGLMSEDMVRQSAKNTFSPVAV
                     RSITDVIAYPESLSNLLN"
     gene            1210..2352
                     /locus_tag="ACHJWA_003927"
     CDS             1210..2352
                     /locus_tag="ACHJWA_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase domain-containing
                     protein"
                     /protein_id="HFO4310437.1"
                     /translation="MSDILEDMIYRRTLSCGTIQVTRDQGEVSLDDLFDIAERRNPKR
                     AFLFVSKVLGRHIPVPPSVMRQAYRQLASQFPSTLTGPVLFIGMAETAVGLGAGVFDE
                     VRHQHRESVYLTSTRHPVDGTLLCEFKEEHSHATDHLIYLPDDEEKRRRVTNARTLVL
                     IDDEATTGNTFINLLSALRNTGKLQHIEQVIAVTLTDWSGNALSERTPLPVTSVSLVS
                     GQWGWTPLPDAPVPDMPKVNVTSRGEWDIQGKQSWGRLGMLAPAADLGHEVSVHKGER
                     ILVLGTGEFVWEPFLLAERLEAAGAQAFYGSTTRSPVAVGYAIESAISFTDNYGLGIP
                     NFVYNVAHQQFDRILVCTETPAESIDTQLLKALAEVAPVVEIVTYE"
     gene            2345..3118
                     /locus_tag="ACHJWA_003928"
     CDS             2345..3118
                     /locus_tag="ACHJWA_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310438.1"
                     /translation="MNKPVVLSDLDDTLFQTRRKMVDELALEPFRTGAVDRTLNPRSF
                     MTEEQSMLVDWLLEQAELIPVTARGTEEISRVRIPFHSWAITTHGAVILTPEGKPDEE
                     WKAHMLGQLAPYQEKLTSMQRLITEMMDAKGINAWARLNFEYGETAVYMVMKHRDSTR
                     LDELNAIADEIETVFPTEGFYIHRNSNNVAWLPTPVEKGLAVSWLLEKLRAERGVFPV
                     IGLGDSLSDHRFMKLCSWFGIPRQSQFADAISQRIFGEN"
     gene            3120..4199
                     /locus_tag="ACHJWA_003929"
     CDS             3120..4199
                     /locus_tag="ACHJWA_003929"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HFO4310439.1"
                     /translation="MQPHETFTGSYQPGDVEFLLKPVVIEMTPVEKKEELIQSGKKHY
                     SDMLSQEPAPTQWHLDLFHRALDRGAERLAKEVTQLAIALAERFGDEPIVLASLVRAG
                     VPLGVMLHQALRDMGKTSWHYGISIIRDRGIDGAALDVIEERHGTSGIVFVDGWTGKG
                     AITGELVRALKDRPGYPEQPRLVVLADPCGCSWLAASDDDWLIPFGIMGAPVSGLISR
                     SVWSSEGLHGCMVCEHLSEFECSRMLADTVAHFRKKLTPSSLAPLSWNTESARVLWQT
                     SRDVIAFLADEFKVDSVNRIKPGIAEATRAVLRRVPDHVFVRSIDDPDVALLVGLARE
                     KGIVVTEMGGTLGQYRAVTIIKKVL"
     gene            4199..5155
                     /locus_tag="ACHJWA_003930"
     CDS             4199..5155
                     /locus_tag="ACHJWA_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HFO4310440.1"
                     /translation="MKNRLSPWNLGATLYMPATREDIADAVLHGKIPGLRSLVICLED
                     AVSEADIPVALKNLEHLLHELSNSMSSLGKNDWPLVFIRPRHAEMGRWLKAHYDLSAV
                     DGFVLPKFTLSSLAEWWDIMVGTHLCMMPTLETEDVFDVVQMRELATRLEEHPCHDRI
                     IALRIGGNDLMNVVSLRRPRDLTLYDSPMGYVIKMLVSVFGPRDFALTAPVCEHIDDH
                     AVMARELALDMAHGLVGKTAIHPGQIEVIQNALMVTQGEHSDALRILNSTQAVFKSQG
                     AMCEPATHRRWAAGILDRARFYGLQNEQSADGIRLLTVTQHH"
     gene            5166..6374
                     /locus_tag="ACHJWA_003931"
     CDS             5166..6374
                     /locus_tag="ACHJWA_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HFO4310441.1"
                     /translation="MGFRFRKSINIIPGVRLNLSNGAPSLSVGPRGASVSFGSRGTYA
                     NLGLPGTGLSYRTRLDRAARSRGENRTATDPGLRQALEQKAAELMSAVTAIRNIHELT
                     PDPKTGISWAELEAVYLHNRTSPFQVPAPVRPEKPDYLVLPEKPAESEGISFLGKWFE
                     SESAKAERHAENLRRWQQELIDVERENTLRQHRYQQQRTAWAEQYANWKFEAEEHEKR
                     LATAQADARQQFRTDAAFFESYLAGVLAETEWPRETLVAFEVKPELSAVLLDVDLAEI
                     EDFPDKIYGVNARGTELTEKAMTQKAVRENYAHHVHGCLFRLVGIVLHTLPFDNVIVS
                     GFTQRVSKRTGYLEDEYILSCKCTRSQMSSVNFAGIKHIDPVEALGDQPVIRKMSSTF
                     IFQPIEPLTL"
     gene            6392..6859
                     /gene="terW"
                     /locus_tag="ACHJWA_003932"
     CDS             6392..6859
                     /gene="terW"
                     /locus_tag="ACHJWA_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance protein TerW"
                     /protein_id="HFO4310442.1"
                     /translation="MQLNTRQARIFKLANLLGTGKPVSAADIITSLECSEPTLTRALK
                     ELRESYSAEIKYSKAGHSYHLVNPGQLDKKTLRRMNEALAQNAELKTGESTGKVVLDK
                     DKKTAVSLSLRMRILRKIDRLAALSDSTRSEAVEKLALHSVDELIKEYSAKKS"
     gene            7120..7449
                     /locus_tag="ACHJWA_003933"
     CDS             7120..7449
                     /locus_tag="ACHJWA_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO4310443.1"
                     /translation="MSKPRWTLDQKKHHVAAWRASGLTREQYCELYDIPFKSLRQWPQ
                     DVAKAEKRARAPEIIPVSVSGSSGMTDGRPLSDEPVTLFLPGGIRMCCQPSQLTDVFR
                     ALRHADA"
     gene            7436..7777
                     /gene="tnpB"
                     /locus_tag="ACHJWA_003934"
     CDS             7436..7777
                     /gene="tnpB"
                     /locus_tag="ACHJWA_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309368.2"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO4310444.1"
                     /translation="MLTPDNVFLVVKPVDMRRGIDTLTQYVQNELNAAWHDGAAFVFT
                     NKVRSRIKVLRWDKHGVWLCTRRLHRGSFRWPRKGDATWHLTQDEFHWLVFGVDWQQV
                     KGHDLAKWVYQ"
     gene            7859..8689
                     /locus_tag="ACHJWA_003935"
                     /pseudo
     CDS             7859..8689
                     /locus_tag="ACHJWA_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(8720..10333)
                     /locus_tag="ACHJWA_003936"
     CDS             complement(8720..10333)
                     /locus_tag="ACHJWA_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element IS682 family transposase"
                     /protein_id="HFO4310445.1"
                     /translation="MSRKYLIRITELERLLSEQAEALRQKDQQLSLVEETEAFLRSAL
                     ARAEEKIEEEEREIEHLRAQIEKLRRMLFGTRSEKLQREVEQAEAQLKQREQESDRYS
                     GREDDPQVPRQLRQSRHRRPLPAHLPREIHRLEPEESCCPECGSELDYLGEVSAEQLE
                     LVSSALKVIRTVRVKKACTKCDCIVEAPAPSRPIERGIAGSGLLARVLTGKYCEHLPL
                     YRQSEIFARQGIELSRALLSNWVDACCQLMTLLNDTLYRYVMNTRKVHTDDTPVKVLA
                     PGRKKAKTGRIWTYVRDDRNAGSSEPPAVWFAYSPDRQGKHPVQHLRPFRGILQADAF
                     SGYDRLFSAEREGGALTEVACWAHARRKIHDVYISSKSATAEEALKRISELYAIEDEI
                     RGLPESERLAVRQQRSKVLLTSLHEWMVEKNGTLSKKSRLGEACSYVLNQWDALCYYS
                     DDGLAEADNNAAERALRAVCLGKKNFMFFGSDHGGERGALLYGLIGTCRLNGIDPEAY
                     LRHILSVLPEWPSNRVDELLPWNVVLTNK"
     gene            complement(10364..10714)
                     /gene="tnpB"
                     /locus_tag="ACHJWA_003937"
     CDS             complement(10364..10714)
                     /gene="tnpB"
                     /locus_tag="ACHJWA_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO4310446.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLDENPFSGHLF
                     IFRGRRGDTIKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            complement(10711..11136)
                     /locus_tag="ACHJWA_003938"
     CDS             complement(10711..11136)
                     /locus_tag="ACHJWA_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO4310447.1"
                     /translation="MEQKILSSEPRRSFSNEFKLQMVKLASQPGASVARIAREHDIND
                     NLLFKWLRLWQNERRISRRLPVTTSSGAGVELLPVEITPDEQKEPMAALTPLLSTPSQ
                     STVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            11190..11941
                     /locus_tag="ACHJWA_003939"
                     /pseudo
     CDS             11190..11941
                     /locus_tag="ACHJWA_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            12281..12454
                     /locus_tag="ACHJWA_003940"
     CDS             12281..12454
                     /locus_tag="ACHJWA_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310448.1"
                     /translation="MSSPEQYLSSSEPASDTSLAVAPAQQNKSTIVRLRSFVCIADDA
                     PEKRVNMSSPEYY"
BASE COUNT         3252 a         3545 c         3507 g         3162 t
ORIGIN      
        1 gttataagga atccaaaaaa cactgacgca ctccataacc acaggagtta ttaatcatat
       61 tatgattgac atgtagctta gacactcata gactcacttc tcaatcataa atttgcatgg
      121 cgttccggat aattaaaaaa aatgaacaag aaaatttggt ttatggaagg tttatcctcc
      181 cagcgagata ttattcaggg ggtaaaatca tttgcacaaa aaaataattt tgccattacc
      241 gtttttgcct cccaccgtaa cgaaagaaat gaaatccttt ccgttgccga ttattctttg
      301 actgaacctg aagatcctca aaaacgtctt cagtttatcc aggaaaccat tcagacctac
      361 ggcatccacc atattcatac tggccgtaac agccagtggt ttgaagaaca ccgttcagcc
      421 attgaaccga ccggtgccac ccttactacc ggtgcaacgg gcgtcgactg gttaactctg
      481 gctgacgaaa aagttacttt tgctcagttt atggagcaaa agggtctccc ggtcgtacca
      541 tcctggcggg tgaatacgct ggcagaatta aagacacacc tcgcggcccc gccgttcact
      601 gacagcccgg tatgcgtgaa gccggtgacg ggtatctatg gcatgggatt ctggcgcttt
      661 gatgacagtg cttcgcctat ggccgtcttt aatcatcccg aacatcgtct ggtcagtccg
      721 caacagtata ttgcagcagc atcagctgct gagtcgttta aaccccttgt tttgatgccg
      781 tacctgccag gcccggaatt ttccgtcgat atcctcgcgg ataagggcga aatactcgca
      841 gccgtgggac gccgtaagga aggggctatc cagtatctgg taaacgaagg aagcgcctgg
      901 gaactggcgt gtgactgcgc ccgtgttatg aaggccgacg ggctggtgaa tgttcagacg
      961 cgaaacgatg tgaatggcaa cccggtgctg cttgaaacca acatgcgtcc gtcagggggg
     1021 gtgggttata cccttcacag cggtgtgaac cttcctgggt tatttgctgc ctttaagctc
     1081 ggtctgatgt ctgaagatat ggtacgccag agcgctaaaa acaccttttc tccggttgcg
     1141 gtgagatcca ttacggatgt aattgcatac ccggaatcac tctctaacct tctgaattaa
     1201 ggcgtattta tgtctgatat cctcgaagat atgatttatc gccgtaccct ctcatgcggt
     1261 acgattcagg taacccgtga ccagggtgaa gtttcgctcg atgacctgtt tgacatcgct
     1321 gaaagacgta acccgaaacg cgcctttctg tttgtcagca aagtactcgg caggcacatt
     1381 cccgtgccac cctcagtcat gcggcaggcc tacagacagc ttgccagcca gttcccctcg
     1441 acactgacag gacccgtact gtttatcggt atggctgaaa ccgccgttgg gcttggggcc
     1501 ggtgtatttg atgaagtgcg ccaccagcat cgtgaatctg tctatctgac ctctacccgt
     1561 cacccggttg atggcacgtt gctttgcgag ttcaaggaag aacacagcca cgccaccgat
     1621 catctgatct atctgccaga tgatgaagag aaaagacgtc gtgttaccaa cgcacgaacg
     1681 ctggttttga ttgatgacga ggcaaccacc ggtaatacct ttattaacct gctttctgcc
     1741 ctgcgtaata ccggcaagct tcaacatatt gaacaggtta tcgccgtaac gctgaccgac
     1801 tggagtggca atgccctgtc ggagcgcacc cccttgcccg tcacttcggt ttcgcttgta
     1861 agcggtcagt ggggatggac cccattacct gatgcacctg tcccggacat gccgaaagtc
     1921 aacgtaactt cacgaggaga atgggacatc caggggaaac agtcatgggg ccgactgggg
     1981 atgctcgcac ctgcggccga tctcggccat gaggtctccg tccacaaggg ggaacgtatt
     2041 ctggttctcg ggaccgggga attcgtctgg gagccgttcc tgcttgctga acggctcgaa
     2101 gctgccggag cacaggcatt ttatggatcg accacccgct cccctgtcgc cgttggttat
     2161 gccattgagt ccgccatttc ctttacggat aactacgggc tgggcatccc caattttgtc
     2221 tataacgtcg cccaccagca gtttgaccgc attcttgtgt gtactgagac acccgcagaa
     2281 agtattgaca cgcagcttct taaagcgctg gctgaggttg cgcccgtcgt ggagattgtt
     2341 acctatgaat aaacccgttg ttctgagcga ccttgacgac acgctctttc agacccgtcg
     2401 taaaatggtg gacgagctgg ccctggagcc attccgtacc ggtgccgttg atcgcaccct
     2461 gaatccacga agctttatga cggaagaaca gtccatgttg gtggactggc tcctggagca
     2521 ggctgaactc atacccgtta ccgcacgggg aactgaagaa atcagccgcg tacggatccc
     2581 tttccactcc tgggcaatca caacgcacgg ggccgtcatt ctcacgcctg aaggcaaacc
     2641 cgacgaggag tggaaggccc atatgctcgg ccagctggct ccctaccagg aaaagctgac
     2701 atcgatgcag cgtctgatca ctgaaatgat ggacgcaaaa ggaatcaatg cctgggcaag
     2761 gctgaacttc gaatacggtg aaacggcggt ttatatggtg atgaaacacc gcgacagcac
     2821 ccgccttgac gagctcaatg ccattgcaga tgagatagaa acggtgtttc cgaccgaggg
     2881 cttctacatc caccgcaaca gtaataacgt ggcctggctt cccaccccgg ttgagaaagg
     2941 gctggcagtc agctggctgc ttgaaaaact tcgggctgaa cgaggagttt tccccgtaat
     3001 tggtttgggc gacagcctga gcgatcatcg ttttatgaaa ctgtgcagct ggtttggcat
     3061 cccgcgtcag agtcagtttg cagatgccat ttcacagcga atttttggag aaaattaaaa
     3121 tgcagccaca tgaaacattt accggctcat accagcccgg tgacgtggaa tttctgctaa
     3181 agccggttgt cattgagatg acgccggttg agaaaaaaga agaactgatt cagtccggga
     3241 agaaacatta ttcggacatg ctcagccagg agccagcgcc tacacaatgg catcttgatt
     3301 tgtttcaccg ggcgctggat cggggcgcag agaggcttgc aaaagaagtc acacagctcg
     3361 ctattgctct ggccgaacgc ttcggtgatg agcccattgt actggccagt cttgtcaggg
     3421 ctggcgttcc gctcggcgtt atgctgcacc aggccctgcg tgacatgggg aaaacctcat
     3481 ggcattacgg tatcagcatt atccgggatc gtggaattga cggtgcagcc ctcgacgtca
     3541 ttgaagagcg gcatggtacc agcggtattg tctttgttga cggatggaca ggtaaaggcg
     3601 caattaccgg agagcttgta cgcgcactga aagatcgccc gggctatcct gagcagccgc
     3661 gactggttgt cctggccgat ccctgtggct gctcctggct tgcagccagt gatgatgact
     3721 ggctcattcc ttttggcatc atgggggcgc cggtatcagg cctgatctcc cggtcagtat
     3781 ggtcctctga aggattacac gggtgcatgg tttgcgagca tctcagtgaa ttcgaatgca
     3841 gccggatgct tgccgatacc gtcgctcatt tccgtaagaa gttaacaccg tcatccctcg
     3901 ctcccctgag ctggaatacg gaatcagccc gggtcttatg gcagacaagt cgcgacgtta
     3961 tcgcgttcct ggccgatgaa tttaaagtgg acagcgtcaa tcgtattaaa cccggtattg
     4021 ctgaagcaac ccgggctgta ttacgtcggg taccggacca tgtatttgtg cgttctattg
     4081 acgacccgga cgttgccttg cttgtagggc ttgctcgtga aaagggaata gttgttacag
     4141 aaatgggggg aaccctcggc cagtatcggg ctgtaaccat tatcaagaag gtactctgat
     4201 gaaaaatcgt ctttctccct ggaatctggg agccacgcta tacatgcctg caacacggga
     4261 agatattgct gatgccgtcc tgcacgggaa aatcccgggc ttgcgctctc tggtgatttg
     4321 cctggaggat gctgtcagtg aagcagacat ccccgtcgcc ctcaaaaatc tggagcacct
     4381 gctgcacgag ctcagtaaca gcatgagtag cctgggtaaa aatgactggc ctctggtgtt
     4441 cattcgcccc cggcatgccg aaatgggcag atggttaaaa gcgcattatg atctttccgc
     4501 tgtcgacggg tttgtgctgc ctaaatttac cctcagttcg ttagctgaat ggtgggatat
     4561 catggtcggg actcacctgt gcatgatgcc aacgctggaa acagaagatg tctttgacgt
     4621 ggttcagatg cgcgagctgg ctacccgtct ggaggaacat ccctgccacg accgcattat
     4681 tgcgcttcga atcggcggca acgatcttat gaacgttgtg tcgcttcgcc gtccccggga
     4741 cctgacgctg tatgacagcc cgatgggcta cgtcatcaaa atgctcgttt cggtcttcgg
     4801 cccgcgtgat tttgccctga ccgcgcctgt atgtgagcat attgacgatc atgccgttat
     4861 ggccagagag ctggctcttg atatggcaca cgggcttgtt gggaaaacgg ccattcaccc
     4921 gggtcagata gaggtcattc aaaacgcgct gatggtcact cagggtgagc attctgacgc
     4981 tctgcggatc ctgaactcta cccaggccgt gtttaagtcg cagggagcaa tgtgtgaacc
     5041 cgccacacat cgccgctggg cggctggcat tctggacagg gctcgttttt atgggttaca
     5101 gaacgagcaa agcgctgatg gaatcagatt acttaccgtg acccagcatc attaaggaaa
     5161 tcataatggg gttcaggttc agaaagtcga tcaacattat tcctggcgtt cgcctcaacc
     5221 tgagtaacgg tgcaccgagc ctgagtgtcg ggccgagagg tgcttccgtt tcttttggta
     5281 gccgggggac ctatgccaat ctgggcttgc ccggtaccgg gctgagttac cgtacccggc
     5341 ttgaccgggc cgcgcgttcc agaggtgaaa accggacggc aaccgacccg gggctcagac
     5401 aggcgcttga gcagaaagcc gctgaactca tgtcagcggt aaccgcaatc cgtaatattc
     5461 acgagctgac gccggatcca aaaacaggca tcagctgggc agagctggaa gcagtatacc
     5521 tgcataacag aacgtcgcct tttcaggttc cggcaccggt gcgtccagaa aagccagact
     5581 accttgtatt gccggaaaag cctgccgaga gcgaaggcat tagttttttg ggtaaatggt
     5641 ttgaatcgga atcagctaaa gctgagcgcc acgccgaaaa tcttcgccgg tggcagcagg
     5701 agctgattga tgtggagcgt gagaataccc ttcgacagca ccggtaccag caacaacgga
     5761 cggcctgggc cgaacagtat gcaaactgga agtttgaagc tgaagaacat gaaaaacggc
     5821 tcgccacggc tcaggcagat gcccggcagc agttccggac agacgccgcg tttttcgaat
     5881 catacctggc gggtgtgctg gcagaaactg aatggccgcg tgaaacgctt gttgcatttg
     5941 aagtaaagcc ggagctatca gcagtcctgc tggacgttga tttagctgag attgaagatt
     6001 tccctgataa gatttacggc gttaatgccc ggggaacgga gctgacggaa aaagccatga
     6061 cgcaaaaagc cgtacgcgaa aactatgccc accacgtcca tggctgcttg ttccgcctgg
     6121 tcggtatcgt tttacatacg ctacctttcg acaacgtgat tgtgtcaggc tttacgcaac
     6181 gggtcagtaa gcggaccggc tatctggagg atgagtatat cctgtcctgc aaatgcactc
     6241 gcagccagat gtcgtcagta aattttgcag gcataaaaca cattgatccg gttgaagcgt
     6301 taggcgatca accggttatt cgaaagatga gcagtacctt catttttcag cctattgaac
     6361 cactaaccct ttaagcaggc taaatcttac gatgcaatta aataccagac aggcccggat
     6421 ttttaagctt gccaacttac tgggcaccgg caagcccgtt tccgctgccg acattataac
     6481 cagtctggaa tgctctgagc cgacattaac ccgggccctc aaagagctac gcgagtcata
     6541 ttctgcggag atcaaataca gcaaggcagg tcattcctat catctcgtta atccgggcca
     6601 gttggataaa aagaccctcc gccggatgaa tgaagcgctc gcacagaatg ctgaactgaa
     6661 aaccggtgag tccacaggga aggtcgtact cgataaggat aaaaaaacag ccgtgtcatt
     6721 atcgctacgc atgcggattc taagaaaaat tgaccgtctg gctgcgctga gtgactcgac
     6781 ccgaagtgaa gcggtagaga agctggctct gcactccgtt gatgaactga taaaagaata
     6841 cagcgctaaa aagtcctgac gctaaatctc agaccggggt gaaaactccg gttttgtcat
     6901 atttcacctc tgtattggcg tgatgcaaaa tggcatctga atcgatcatg gtttggcatc
     6961 catttccgat cactgtttgg catcacaatc gatcacgatt tggcatgctt ccgctcattg
     7021 attggcatcc tggtgtcgtt acggggaagt catcttcaca gggatcttca gtcagttagc
     7081 attaccgaca tccattcacc aacttaccgg agataatcaa tgtcaaaacc ccgatggacg
     7141 cttgatcaga aaaaacatca cgttgccgca tggcgcgcca gcggccttac ccgtgaacag
     7201 tactgtgaac tttacgacat tccgtttaaa tccctgcgcc agtggccgca ggatgtcgca
     7261 aaagccgaaa aacgagccag agcaccagaa attattcctg tttcagtatc tggctcttcc
     7321 ggaatgactg atggcagacc attgtcggat gagcctgtca ctctcttcct gcccggtggt
     7381 atccggatgt gctgccagcc ttctcagtta accgatgttt tccgggctct tcgccatgct
     7441 gacgcctgat aacgtattcc tggtcgtaaa acccgtcgat atgcgccggg gtatcgacac
     7501 actgacacag tacgtccaga acgaacttaa cgctgcatgg catgacggtg ctgcttttgt
     7561 cttcaccaat aaagtccgct cccgcataaa agttctccgg tgggacaaac atggcgtatg
     7621 gctgtgtacc cgacgactgc accggggaag cttccgctgg ccccggaaag gtgatgccac
     7681 atggcatctc acacaggatg agtttcactg gctggtcttt ggggttgact ggcaacaggt
     7741 gaaagggcat gacctggcaa aatgggtata ccagtaagaa cccgaagatg ctcagagcaa
     7801 cgcaataact caatgaaata taatgaatat ccatgcgtga tgtcatataa tccaccgcat
     7861 ggatatctcg cttctcagta ccaccagtga ccctgaacag cttcgcgctc tggcaattgc
     7921 catggtgcaa aaagtcatgg ctgagaatgc agaacttcag aaccgtatcc gcatcctgga
     7981 agagcagatg aagctcgcac gccagcagcg tttcggtaaa aaatgcgaat cgctggccgg
     8041 gatgcagcgt tccctgttcg aagaggatgt tgacgcggat atcgcagaaa tctcggcgca
     8101 tctggacaaa ctgcttcctc agaccgggga cgaagaaaaa acaacaacac gtcctgttcg
     8161 taagcccttg ccttcacctc ttccccgtgc tgaaaaggtt atcccgccag ctgaggaacg
     8221 ttgtcctgac tgtgatgccc ccctgcactt cattcgcgat gaagtcagcg agaagctgga
     8281 atatattccg gcacaggtcg tggtcaaccg ctatatccgc ccgcagtaca gctgtccctg
     8341 ctgtgagaaa gtgttcagcg gaaaaatgcc ggcacatatc cttccgaaaa gtgctgttga
     8401 gccatcggtc atcgcacagg ttgtcatcag caaatatacc gaccatctgc ccctgtatcg
     8461 ccagcagcac attttctccc gaatgggggt ggagctcccc gtcagtacaa tggcggatat
     8521 ggtcggtgtt gctggcgctg cactggcccc gctggcaaaa ttgttacgcc acgagcttct
     8581 gacacgggat gttattcatg cagatgaaac gtccctgagg ctccttgata cccggaaagg
     8641 cggtaaatcc tgctccggct ggctgtgggc ttacgtcagc ggagaaagag taagcgtcaa
     8701 cggagcaccg tattgacgct tatttattgg tgagtactac gttccatggc aggagttcat
     8761 caactcggtt ggaaggccat tctggcagta cgctcaggat atggcgcaga tacgcttccg
     8821 gatcgatacc gttcagacgg caggtgccga tcagcccgta caacagtgct ccacgctcgc
     8881 cgccgtgatc gctgccaaag aacataaagt ttttctttcc gagacagact gcacgaagcg
     8941 ctctttccgc agcattatta tccgcctccg ccagaccgtc atcactgtaa taacagaggg
     9001 catcccactg attcagtaca tagctgcacg cttcgcccag tctggatttt ttcgacagcg
     9061 taccattctt ctccaccatc cattcatgca gtgacgtcag taacactttg cttcgctgct
     9121 gcctgacggc aagacgctct gactccggta atccccgtat ttcatcctcg atggcgtaca
     9181 gttcactgat tcgcttcagg gcttcttctg ccgtcgcact tttgctgctg atgtatacat
     9241 cgtggatttt tcgccgggca tgggcccagc acgcaacttc tgtcagtgca ccaccttcac
     9301 gttctgcact gaacagccga tcgtaaccgc tgaacgcatc cgcctgcagg ataccccgga
     9361 agggacgaag gtgttgtacc ggatgttttc cctgcctgtc tggtgagtag gcgaaccaga
     9421 ccgccggtgg ctctgatgag cccgcattcc ggtcatcccg gacatacgtc cagatgcgtc
     9481 ctgtttttgc cttttttctg cccggtgcca gcacttttac tggtgtgtcg tcagtgtgaa
     9541 ccttgcgggt gttcatcacg taacggtaca gggtatcatt cagcagagtc attaactgac
     9601 aacatgcgtc aacccagttg gagagtaatg cccggctcag ttcgatgccc tggcgggcaa
     9661 agatttcact ctgacgatac agtggcaggt gttcgcagta ttttcccgtt aacacgcggg
     9721 caagtaatcc ggagcccgcg atgccgcgct ctatcgggcg ggacggcgct ggcgcttcaa
     9781 ctatacagtc acattttgta caggcttttt ttacccgaac agtgcggatc actttcaggg
     9841 cgctactcac cagttccagc tgctcagcac tgacttcccc cagataatcc agctcactgc
     9901 cacactccgg gcaacaactt tcttcaggct ccagacggtg tatttcacgg ggaagatgtg
     9961 ccggtaacgg acgacgatga cgtgactgtc gcaactggcg gggaacctgc gggtcatcct
    10021 cacgcccact gtaacgatcg ctttcctgtt cgcgttgttt cagttgggcc tcagcctgtt
    10081 caacctcacg ctgcagtttt tcagaacggg taccgaacaa catccggcgc agtttttcta
    10141 tctgggccct cagatgttct atttcccgct cctcctcttc gatcttttcc tcggcacgtg
    10201 ccagtgcaga gcgcaggaag gcctccgtct cttcaaccag actcagttgc tgatctttct
    10261 gacggagggc ttcagcctgc tcagagagca gcctttccag ctcagtgata cgaatgaggt
    10321 atttccgact catgaccgtt tttataatcc ggccatgaca tttttacaac attgtcagtg
    10381 cattaaggcg ggatgttttg ggctgacgcc agtccagttt atcgaggagc attgccagct
    10441 gcgagcgggt aatggatacc ttaccgtcac gcaccgcagg ccagataaac tggccttcct
    10501 ccagacgttt ggtgaacagg cacagaccat cagcatcagc ccacagaatt ttaatcgtgt
    10561 caccccgtcg gccgcgaaag ataaacaggt gaccggagaa ggggttctca tccagcacat
    10621 gttgtacctg ttctcccaga ccgttgaagg atttacgcat atcagtaacg ccggcaacca
    10681 gccagattcg agtgcctgat gggagcgaga tcatcgtccc ctcccggtca gttcacggat
    10741 caacaccgtg agcagctctg gtgaaggatt ttccagcgtc atgttaccgt ggcggaactc
    10801 aactttacag gaactggcac tgactgtgct ttgtgaagga gtggataaaa gcggagtaag
    10861 agccgccata ggctctttct gctcatcagg cgttatctca acaggtaata attcaacgcc
    10921 agcgccagaa gaggttgtta ccggaagacg ccgcgatata cgcctttcgt tctgccagag
    10981 cctgagccat ttgaacagga ggttatcatt gatatcgtgt tcccgggcaa tacgggcaac
    11041 agaggctcct ggttgtgaag ccagtttaac catttgaagt ttaaactcat ttgaaaatga
    11101 tctgcggggt tctgaggata atattttctg ttccataaca ggtgtccact agttgaaaag
    11161 gtgggcacct acgttaccaa tactggctta atggctacat acggcggtca gtttacgctt
    11221 acgagaaaga agcggtccgc cggtcgtctg ttttgactcg cagaccggac gggccctcag
    11281 atatcctgag gcctggctgc agggctggtg tggcggtacg ctggtgagtg acggatacag
    11341 cgtgtacaaa tctctggcgg ataatgaccc cggtattaca tctgcctgtt gctggagcca
    11401 cgcaaggcgc ggtttcgcaa atctgtataa ggccagcagg gagccccgtg cagccatggc
    11461 gctgagaaaa atcgcgggtc tttatcgcat tgagaagttc atccgtgaac gtcctgtgga
    11521 aaaaatacgg caatggcgac aacggtattc ccggccgata gtgaatgacc tgtttgcctg
    11581 gcctgaagag caggagccgt gctgtccgcc cgacggacca ctaaacaaag cgattaatta
    11641 tatcctgaac cggcgcgatg aactgagctg cttcctgagt gacggagccg tgcccctgga
    11701 caacaatatc tgcgagcgtg ctatccgccc ggtggtcatg gggcggaaag catggttatt
    11761 cgccggttca ctgatggccg ggaaccgcgc ggcacagata atgagccttc tggaaacggc
    11821 aaaacgtaat ggtctggagc cgcatgcctg gctgacagac gttctgacgc gtctaccgga
    11881 gtggccggaa gaccgactgg aagagttgct gccccttgag ggatttacct tctccgggtg
    11941 agtgatacct gccatcaggt gttcgtgcag cgggccataa cccgcagtcg ggagttgaac
    12001 gcctgacgac aggaaatgag ccagagcagc gaccacgcgg gctgacagtg agtcagaacc
    12061 tgaatcagcc cggagccatt cccggggctg tacttcatca cctgccatca gtacccgtgt
    12121 cagtacttcg cgacctgacg caattacccg actcagttct tcagaacccg cctccgatac
    12181 cagcctggcg gtcgcccctg tgcaacaaaa taaatcaacc atcgtcagac ggtgatcaat
    12241 cgtatgcgct gccagtggct cgcctgaaaa atgcgtcaat atgagttcgc cggaacaata
    12301 cctcagttct tcagaacccg cctccgatac cagcctggcg gtcgccccgg cgcaacagaa
    12361 taaatcaacc atcgtcagac tgcgatcatt cgtatgcatc gctgatgatg cgcccgaaaa
    12421 acgcgtcaat atgagttcgc cggaatatta ctgacgacac ggtttgtagt tacgttcctt
    12481 cggtgagtga gtacctgtgc gtcagggtaa cgcaatcagt tcaggtttag cggggtgtca
    12541 agtcggtttg gctaaccgac cgcagaatta actgtacctt taaaggtcgc ccccgatata
    12601 tctgcctgtc agacatatcg ccgattcggt atctcactac ataaaactcc ttaaaaaaca
    12661 atgcgattct gttggtacga agctttcagt aagtcagtcc ggagaagcgc cagtacgata
    12721 cagaatgagg ttgaaaaaaa cgttcacgtc tggtcacttc ttcaaatgtt aaaaaacatc
    12781 tcgtaaagag ccagtttatg cgggttcgtt gagtagacgt gcgagcgtca ctttgtcagc
    12841 tttaacgctg acgaaagtga cgcccgcaca gcttcaaact ccacatgcac acgcaagcga
    12901 ccaaatttca aatggtctta tgccgtgcgc acagtcataa gaagcgcatg cggaaagtca
    12961 gaaaatatct taagacgcag attttaaagg cgtttttatg ccagtctgcc gggaatacac
    13021 agtagtccgg ttgtccggaa gcaccacggt aatccgaagg aaaaatcaac gtacctcaat
    13081 caccgtagtg atatccgttc ccagaggcta aacggatgta ccaccataaa ggcatcctct
    13141 ttcaggctac gaggatattg caatcacccg aaggcgtttc tctcagattg ctgaaacatt
    13201 ggctggcagt cacccgaagg cgtccctctc agactgctgg aacattggct ggtcgcaaaa
    13261 gcgacagtta atgcaaatac aggttaacaa aaagggaaat ctgccgtcaa cccaaccata
    13321 tgaacatgtt gatttcatta tgaatgtgat gttctaatat aaattaatca gtaacttcat
    13381 aaaaagaata taagaaacag ataatgcatg tatttacaca catatcagca caatgataga
    13441 ttaccacccc agaaacacga taaagg
//
LOCUS       DAZFAB010000117        13326 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZFAB010000117 DAZFAB010000000
VERSION     DAZFAB010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.117"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..843
                     /locus_tag="ACHJWA_003941"
     CDS             <1..843
                     /locus_tag="ACHJWA_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFO4310449.1"
                     /translation="LSVNRLVYFSLLKKLFQAMNLCQIPGRAGALLTTLLRHISTING
                     FDNLMAQPLLSDEPLTALMDHYLDTDALADGLPLYVSLYPTEGGMQDIIDCIRAELGV
                     GTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQGTMFGDGGMGGWRNMQGNT
                     PVTPLVDAGCNMVIVTHLSDGSLWDRQAFPDTTILEIRPRKRLKYAGDGGNSGGLLSF
                     TSAHTDAWRQQGYEDTMLAMEHIRKPLAARQALTRSEAVLQKSLDITEEADLALRNAM
                     ARIK"
     gene            979..1662
                     /locus_tag="ACHJWA_003942"
     CDS             979..1662
                     /locus_tag="ACHJWA_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310450.1"
                     /translation="MKLPVKLLMSLISLVRVIARAGEYKNYSRDEIKYWRYTSYKGGK
                     LPEGFTDEKFSSAIYNGRIFTMKRLHTLMLFLAVLFTGFNFNAEAATVKQALSCNPEA
                     WAEQPGACPSTYELYEGDATYKAAIDKALKPVGLSGMFGKGGYMDGPGGGITPVNING
                     TVWFQGDGCKANTCGWDFIVTLYNPKTHEVVGYRYFGLDDPAYLVWFGEIGVHEFAYL
                     VKNYVAAVN"
     gene            1678..2088
                     /locus_tag="ACHJWA_003943"
     CDS             1678..2088
                     /locus_tag="ACHJWA_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310451.1"
                     /translation="MKTFIKTLLVAVTILFSVFATAKQVKLPNNIKYVNTTEAFSCTE
                     IDGMNCQTKNQFNYKDNSYVFVLERGGAWCYDYTVSVVNLKTGKAQMIEYGDNQLCSG
                     SNKPFFEIKNGVPTVGVIDTSGKPVGVAQDKLKI"
     gene            2309..3130
                     /locus_tag="ACHJWA_003944"
     CDS             2309..3130
                     /locus_tag="ACHJWA_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO4310452.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELIRHVPSIFGEDRHTSRSER
                     YAYIPTITVLENLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEII
                     LLNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPQPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETMLESLR"
     gene            3212..3691
                     /locus_tag="ACHJWA_003945"
     CDS             3212..3691
                     /locus_tag="ACHJWA_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO4310453.1"
                     /translation="MKTVSQNTPTIYSATTPENNPPQLVASLVPDEQRISFWPQHFGL
                     IPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMN
                     GNRAEMSPEAAGIAACLMTYSHHACRTENYAMTVHYYRLRDYALQHPEGSAIMRIID"
     gene            3706..4182
                     /gene="yeeS"
                     /locus_tag="ACHJWA_003946"
     CDS             3706..4182
                     /gene="yeeS"
                     /locus_tag="ACHJWA_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFO4310454.1"
                     /translation="MQQISFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            4245..4466
                     /gene="yeeT"
                     /locus_tag="ACHJWA_003947"
     CDS             4245..4466
                     /gene="yeeT"
                     /locus_tag="ACHJWA_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO4310455.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            4540..4908
                     /gene="cbeA"
                     /locus_tag="ACHJWA_003948"
     CDS             4540..4908
                     /gene="cbeA"
                     /locus_tag="ACHJWA_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system cytoskeleton
                     bundling-enhancing antitoxin CbeA"
                     /protein_id="HFO4310456.1"
                     /translation="MSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHPDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLSSCGYVYLAVYPTPEMKN"
     gene            4997..5370
                     /gene="cbtA"
                     /locus_tag="ACHJWA_003949"
                     /pseudo
     CDS             4997..5370
                     /gene="cbtA"
                     /locus_tag="ACHJWA_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system
                     cytoskeleton-binding toxin CbtA"
     gene            5367..5561
                     /gene="yeeW"
                     /locus_tag="ACHJWA_003950"
     CDS             5367..5561
                     /gene="yeeW"
                     /locus_tag="ACHJWA_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO4310457.1"
                     /translation="MMTLEADSVNVQALDMGHIVVDIDGVNITELINKAAENGYSLRV
                     VDDRDSTETPATYASPHQLL"
     gene            5607..5687
                     /locus_tag="ACHJWA_003951"
                     /pseudo
     CDS             5607..5687
                     /locus_tag="ACHJWA_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5976..6120)
                     /locus_tag="ACHJWA_003952"
                     /pseudo
     CDS             complement(5976..6120)
                     /locus_tag="ACHJWA_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            6174..6356
                     /locus_tag="ACHJWA_003953"
     CDS             6174..6356
                     /locus_tag="ACHJWA_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFO4310458.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKCWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCR"
     gene            complement(6457..6786)
                     /gene="tmaR"
                     /locus_tag="ACHJWA_003954"
     CDS             complement(6457..6786)
                     /gene="tmaR"
                     /locus_tag="ACHJWA_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFO4310459.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(6958..8016)
                     /gene="yeeA"
                     /locus_tag="ACHJWA_003955"
     CDS             complement(6958..8016)
                     /gene="yeeA"
                     /locus_tag="ACHJWA_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFO4310460.1"
                     /translation="MRANKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     GTWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSSLICRALRK"
     gene            complement(8215..8688)
                     /gene="sbmC"
                     /locus_tag="ACHJWA_003956"
     CDS             complement(8215..8688)
                     /gene="sbmC"
                     /locus_tag="ACHJWA_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFO4310461.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(8807..9973)
                     /gene="dacD"
                     /locus_tag="ACHJWA_003957"
     CDS             complement(8807..9973)
                     /gene="dacD"
                     /locus_tag="ACHJWA_003957"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFO4310462.1"
                     /translation="MKRRLIIAASLFIFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIALGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            complement(10813..10970)
                     /locus_tag="ACHJWA_003958"
                     /pseudo
     CDS             complement(10813..10970)
                     /locus_tag="ACHJWA_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701877.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
     gene            11298..11948
                     /gene="nleG7"
                     /locus_tag="ACHJWA_003959"
     CDS             11298..11948
                     /gene="nleG7"
                     /locus_tag="ACHJWA_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector NleG7"
                     /protein_id="HFO4310463.1"
                     /translation="MPIFLNFTAGSILPENELASLRYIVQQNQNDTVIIKERYKMDIR
                     YIESVNGFTVNPVCSNHFSIFMARQNTIARNLEQQINNGRSFAQISQDFMLQLSSNIG
                     WKKGAENALKNKIHSHSFVVNPDEFSCDTQFLKCPITLCVPEKGVFVKNALNSNICTL
                     YDKSAFMNLTREHLPHPLSREKIVKEMIIERNMCYFDTISQHFIIMDTDQQKQHCK"
     gene            complement(12173..13048)
                     /locus_tag="ACHJWA_003960"
     CDS             complement(12173..13048)
                     /locus_tag="ACHJWA_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspS"
                     /protein_id="HFO4310464.1"
                     /translation="MFSIRTLLPISASVSVPTKQSQSIPITLAGRTIEKAQEKEGLLV
                     FLGMKSVNDYTLNILGQNVSRVTTGKKPYDLLFLNDATKQDFDKRKMEFTYPGANKSH
                     LQSSNSDVVAAAAISITATEMKTILPDDLTLGKYNKIYLSGHGSAGLPLLKCGDEFLS
                     PSDIVDRIVQHNLHEIDDIRLTSCNSANIIKNKDFSPDEIEKSANMNNGWLARALFGQ
                     KRSLAEHVYAEFERRGINVSISGYHGTGVFYVPEHGKPTTHLRSTTVPATPEHTVRRS
                     DYRATLGRTQPIDIE"
     gene            complement(13189..>13326)
                     /locus_tag="ACHJWA_003961"
     CDS             complement(13189..>13326)
                     /locus_tag="ACHJWA_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO4310465.1"
                     /translation="IIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGRWENIGGM
                     K"
BASE COUNT         3560 a         3276 c         3037 g         3453 t
ORIGIN      
        1 ctgtcggtaa acagattggt ttacttttca ttgctgaaaa aattgttcca ggcaatgaac
       61 ctctgccaga tccccggacg tgcaggagca ctgcttacga cgcttcttcg ccatatatcg
      121 acaatcaacg ggtttgacaa tctgatggct cagccgttgt tgtcagatga gcccctgaca
      181 gcgctgatgg atcattatct tgatactgat gctctggcag acgggctacc gctgtatgtg
      241 tcgctgtacc ccacagaagg gggcatgcag gatattattg actgcattcg tgctgaactg
      301 ggtgtcggaa ccacgaaaaa cgccgttttt cagcatatcc agagcctgcc ccgcggacag
      361 cagaaagagg ctctgcttgc gtcagccgcg ctgcccctgc tgttccgtcc ccgtgaggtt
      421 caggggacaa tgttcggtga tggtggtatg ggaggatggc gaaatatgca gggaaatacc
      481 cctgtgacgc ctctggtcga tgccggatgc aatatggtga ttgtgacgca tctgagtgac
      541 ggttctttat gggatcgcca ggcttttccg gacaccacaa tccttgagat ccgtccccgg
      601 aaaaggctga aatatgcagg tgatggtggc aacagcggcg gtctgctcag ttttacatcg
      661 gcacataccg acgcctggcg tcagcagggc tatgaagaca cgatgctggc gatggagcat
      721 atccggaaac cgctggcagc acgtcaggca ctgacccggt cagaggcggt attgcagaaa
      781 agcctggata taacggaaga ggcagatttg gcactgagaa acgcgatggc ccggattaaa
      841 taaagacgcc ccaaagaaac gccaccgcct gtggtggtgt ttttcctgtc tggtgttatt
      901 gaaaatattt tcagatatat attacctgag agaaagtttt acccctcacc tgtcaattca
      961 ctcactggaa atttaattat gaaattacct gtaaagttat taatgagcct tatatctctg
     1021 gtccgcgtta ttgcacgtgc cggggaatat aaaaattaca gccgggatga aataaaatac
     1081 tggcgatata catcatacaa gggggggaaa cttccggaag gtttcactga tgagaaattt
     1141 tccagcgcca tttacaacgg aagaatattt acaatgaaac gtttacatac ccttatgtta
     1201 tttctggcgg ttctgttcac gggctttaac tttaacgcag aagcggctac cgtaaaacaa
     1261 gctcttagtt gtaacccgga agcctgggct gaacaacctg gagcgtgtcc atcaacgtac
     1321 gagttgtacg aaggtgacgc tacctacaaa gccgctatcg acaaagcatt aaaaccggtc
     1381 ggactgagcg gcatgttcgg taaaggcggc tatatggatg gccctggtgg aggtatcacg
     1441 ccagtaaaca ttaacggtac agtctggttc cagggcgacg gttgcaaagc caatacctgc
     1501 ggctgggact ttatcgtaac actctataac ccaaaaaccc atgaagttgt tggctaccgt
     1561 tactttggtt tagatgaccc ggcctacctg gtttggttcg gtgaaattgg cgtgcatgaa
     1621 ttcgcgtatc tggtgaaaaa ctacgtagct gcggttaact aatttaagga gtctatcatg
     1681 aaaactttta tcaaaacttt actcgttgct gtaactattc tgttctctgt cttcgctact
     1741 gcgaaacaag taaaactgcc aaataacatc aaatacgtta atactacaga agcgttttcc
     1801 tgtaccgaaa ttgacggtat gaattgccag acaaagaatc agttcaacta taaagataac
     1861 agctatgttt tcgtgcttga acgtggtggt gcctggtgct acgactacac tgtctcggta
     1921 gttaacctga aaaccgggaa agcacaaatg attgaatacg gggacaatca actgtgctca
     1981 ggtagcaaca aaccgttctt cgaaatcaaa aatggcgtac cgacggtagg agtcatcgac
     2041 acatccggaa aacctgtcgg tgtagcccag gacaaactta aaatctaagg agtaaaccat
     2101 gcgtttctct gcacattatt aatatcaaag gtaaatacag ccccagccat tgttaccgga
     2161 aagccgctta atgcgtggct cgccagtgaa aggcctctca gacaataaac cttattcatt
     2221 tcacccgtca ggccgcgtct tctccgggag acgcggcttt tttcatttat tgcaccagta
     2281 aatcttaacc accgataagg agcaaagtat gcgattagcc agtcgttttg gttatgctgc
     2341 aaaccagata cgccgtgacc gtccgctgac acatgaagaa ctgatacgcc atgtacccag
     2401 tatttttggg gaagaccggc acacctcccg cagtgaacgg tatgcgtaca ttcccaccat
     2461 taccgtcctg gaaaatctgc agcgggaagg ctttcagccg ttcttcgcct gccagacccg
     2521 tgtgcgcgac ccgggccgcc ggggatacac aaaacacatg ctgcgtctgc ggcgggccgg
     2581 agagataaac ggagaacatg tccctgaaat tattctgctc aactctcatg acggtacctc
     2641 cagctaccag atgctgccgg gttacttcag gttcgtctgc cagaacgggt gcgtctgtgg
     2701 ccagtctctg ggggaagtgc gtgttccaca ccggggaaat gtagtggaga aagttatcga
     2761 aggggcttac gaggtggtgg gcgtgtttga ccggatagag gagaagcgtg atgccatgca
     2821 gtcgctggtc ctgccgcaac cggcacgcca ggcgctggca caggcggcac tgacttaccg
     2881 ttatggtgac gaacatcagc ccgtcaccac cgccgacatt ctgacgccgc gacgccggga
     2941 ggattacggt aaggacctgt ggagcgcata tcagaccatc caggagaata tgctgaaagg
     3001 cgggatttcc ggtcgcagtg caaaaggaaa acgtatccat acccgggcca ttcacagcat
     3061 tgataccgac attaagctca atcgcgcatt gtgggtgatg gcagaaacga tgctggagag
     3121 cctgcgctga tgccgtttcc ctgagtgagg gaatcattca gctttcgttc ccgaaaaaca
     3181 cacatttatt tgttatttaa ggagttatct catgaaaacc gtttctcaga atacccccac
     3241 aatttattca gctacaacac cagagaataa tccgcctcag ttggttgcca gcctcgtccc
     3301 tgatgaacag cgcatcagct tctggccgca gcattttggc ctcattccac agtgggtcac
     3361 cctggagccc cgtgtcttcg gctggatgga ccgtctgtgc gaagactact gcggtggtat
     3421 ctggaatctg tacaccctga acaacggcgg ggcatttatg gcccccgaac cggatgatga
     3481 tgatgacgaa acatgggtac tgttcaatgc catgaacggt aaccgcgctg aaatgagccc
     3541 ggaagccgcc ggtattgccg cctgtctgat gacgtacagc catcatgcct gtcgtacgga
     3601 gaattatgcc atgacggtcc attattaccg gttgcgggat tacgccctgc agcatccgga
     3661 aggcagcgcc attatgcgca tcattgactg aaaggggccg gaataatgca acagatttcc
     3721 tttctgcccg gagaaatgac gcccggcgag cgcagcctca ttcaacgggc cctgaaaacc
     3781 ctggaccgcc atcttcatga acccggtgtg gccttcacct ccacccgtgc agcacgggaa
     3841 tggctgattc tgaacatggc gggactggag cgtgaagagt tccgggtgct gtatctgaac
     3901 aaccagaatc agctgattgc cggtgaaacc ctcttcaccg gcaccatcaa ccgcacggaa
     3961 gtccatcccc gggaagtgat taaacgcgcc ctgtaccaca atgccgctgc cgtggtactg
     4021 gcacacaatc acccgtccgg tgaagtcaca cccagtaagg cagaccggct tatcaccgaa
     4081 cgtctggtac aggcactggg cctggtggat atccgggtgc cggaccatct gatagtcggt
     4141 ggcaaccagg ttttctcctt tgccgaacat ggtctgcttt aacccgtcac aaccacatca
     4201 cacctgtttt cacttttatc ttctgtcttc agaggtatcc cattatgaaa attatcaccc
     4261 gtggtgaagc catgcgtatt caccaacaac atccgacatc ccgtcttttt ccgttctgta
     4321 ccggtaaata ccgctggcac ggcagtgctg aagcgtatac cggtcgtgaa gtgcaggata
     4381 ttcccggtgt gctggccgtg tttgctgaac gccgtaagga cagttttggt ccgtatgtcc
     4441 ggctgatgag cgtcaccctg aactgagtgg gaattctgat gagcagaatt atcgccacta
     4501 ccggaccatt cttagccgat tttctgtaag gattttatcg tgtcagacac actccccggg
     4561 acaacacttc ccgacgacaa tcacgaccgc ccctggtggg ggctgccctg caccgtgacg
     4621 ccctgtttcg gggcacgtct ggtgcaggag ggtaaccggt tgcattacct tgccgaccgc
     4681 gccggtatca gaggcctgtt cagcgatgca gatgcgtacc acccggacca ggcctttccg
     4741 ctgctgatga aacaactgga actcatgctc accagcggtg aactgaatcc ccgccatcag
     4801 cataccgtca cgctgtatgc aaaagggctg acctgcaaag ccgataccct cagcagttgt
     4861 ggttacgttt atctggctgt ttatccgacg cccgaaatga aaaattaact ctccagaata
     4921 gccttctgct acggcctggt gttttcacca cgccactttt ccatttttat atctgcatat
     4981 caggaaaatc ttcagtatga aaacattacc tgtattaccc gggcaggcgg ccagttctcg
     5041 cccgtctcct gttgaaatct ggcagatact gctgtcccga ctgctggacc agcactatgg
     5101 cctcacactg aatgacacac cttttgccga tgaacgtgtg attgagcagc atattgaggc
     5161 aggcatttca ctgtgtgatg cggtgaactt tctcgtggaa aaatacgcgc tggtgctacc
     5221 gaccagccgg gattcagcgc ctgtacccgc tctcagttaa taaacagcat cgatatcctc
     5281 cgggctcgca gggcgaccgg cctgatgacc cgcgacaatt acagaacggt aaataacatt
     5341 accctgggta agtatccgga ggcgaaatga tgacactgga agccgacagc gttaacgtac
     5401 aggcgctgga tatggggcac attgtcgttg acattgatgg tgttaatatc actgaactga
     5461 ttaataaggc cgctgaaaac ggttattcac tccgcgtggt ggatgaccgt gactctaccg
     5521 aaacaccggc aacttatgcc agccctcacc agttgctgta agacaatgca gtgatgcagg
     5581 gtatagtgcc attaaccaca gaaacagcac tgttgtcctg tattaagcag gcaagagaag
     5641 atgcccttac cctgcgccat ctgcagcttc tgcaccagaa ccggtgaatc tcactcaaca
     5701 ggcttcattt cccctgacgc cagcctgaat acagctggcg ttttcattta taaacagaaa
     5761 ggaaaaccga taataatgga atggacgccc cgaccatgaa gcaacggtag gatattactg
     5821 ataaaccttc cagtggaggt gcgttatggc cgttttaacg attagcattg acctgacaaa
     5881 aaatgaattc caaatccacg gtctgggtag gaacagaaaa atctaagctc agaaaacgga
     5941 ttaaagtaag cgtcaacgga gcaccgtatt gacgcttatt tattggtgag aactacgttc
     6001 catggcagga gttcgtcaac acggttggag ggccattccg gcagtatgct cagaatatgg
     6061 cgcagatacg cttccggttc aaccgttcag acggcaggtg cctgcttttc cggcaacaag
     6121 caggaatctc agcgctgaag caggaattag aggtaaaaac gccctaccgg gccatgaatc
     6181 acccggtcat tggagtagta accaaagcag atttagccag catggaacaa atatctttgg
     6241 tgaaatgttg gttacgggaa gctggagcgc acaacgtgtt agtaaccagt gcggttaata
     6301 acaatggagt tacagaactt tttgccttgc tgcatacaga agaaggctgc cgctaatttc
     6361 gataggttct cagatgagaa tgatgacggt gatacacaga taagaatcaa tgagcaatga
     6421 gggttgccgg gcaaccctca ttgaataaaa caggaattac ttcgcttcgc cgtttttcat
     6481 ttcgcccata gctttcagct ttttggagat atcgcggcgt tctttggaga gctcggcatt
     6541 tttgatgatg taatcgtcaa cgcgatcttc atagtcacct ttcatgctgg cgatgatgcc
     6601 ctggattgct tcaacgctca tccctggctt gatgtaatcg ctcaggttgt ccagcaacag
     6661 gacgcgcttc tggttgtcac ggatcttttt ctcaacgtcc tgaatttcac gttgcagttt
     6721 gttcttacga cggaacagac gaacaaattc cagtacgtcc tggaatgaag gcttggtagt
     6781 ttccattttt atacccctga taatgtgaga gtcggattcg tttaagcaac cgctattcgt
     6841 tagggccaac attactcata gctgccgcag atgacaatgc ttttatccct tatcactatc
     6901 atacccttta tccttgctga atcgaagcag cagcaagatg attctgaagt tcaggaatta
     6961 tttgcgcaag gcccggcaaa tcagactcga tagcaactca agctgatgtg ccgtttccat
     7021 gttcagccac acataaccat agattggtgt ttccacaacc ttcaggtcat ggtggttatt
     7081 cagcaactga cgcagctctt ccacagcatc gttcaatttt tccgtattgg caaaaaccgg
     7141 ctgcggatta ccttcgtaca gcgcatgaac aaggctcagc agtatttgct gcatcatgtg
     7201 ctgggtatca cgaagttttt gcgcgttcaa taacacgaaa tggctggggc gcgtggccca
     7261 gtatgcattg atttgcaact ccagcataca aaccagattg cggttaatgg tctggatacc
     7321 ttcatatatc gattttggaa tacgagtttc tttgctggcg ggcgcaatca gcccacgcat
     7381 tttcacggca tcggtcagga gtttttgtag atggctttcc agacgtgggc gttcgagtaa
     7441 gttcggtgag aatgcagatt gatagacccg attatactcg gtcagacttt tcgccagttg
     7501 aatgcgccag tggatgaacg cccgttgtgg ccagataccg gtaaacaaca ttgccagtaa
     7561 agagccgagg atcacatcgc cgcttcgcca taacgccgta tcaatttcac ctgtcgggga
     7621 acccacaaca attgccagcg tcaccccaat caataatcct tgatacggtt tcttgcccag
     7681 cgccagccaa ccgcaaagaa acatcgccgc cgcgcaccag actaacatca gcggtaacga
     7741 aattaactcc agttgcagag cgataagacc taaaatcgac cccaacaccg taccgccaat
     7801 acgttcgaag gcacgaggga caacgttccc ccagaacgag attggcccca taatcaccac
     7861 catggtgacc agcggccagg tgccttccgg gatagtaaac aggcggataa tgagaaaagt
     7921 gagcaggaat gccagcgcga cccgagtacc atgcacaatg cggtaatggc ggtatacccg
     7981 gatttcaaac gggcttaatg acttattggc acgcacgcag aaactccgtt aatgaagatt
     8041 aaaagcgctc ataacacatc tgtcggtcag aaggcgcgcc tctgaattat ccgacggaca
     8101 aaccgggaac agtgtaattt cccggcagga gcgaaaaaat tgaaaggcgc atcttactct
     8161 tttcgctttc aaaaaaagag tggtcttcgc gtcaacacac cgccctgaga tgaattagtg
     8221 atgttttggc tgcaccgcaa catacatttc gatatcccag tacccatctt ccgcgccatt
     8281 gttcaagtaa acctcgaagc agggctttgg taacatttca taagcactgt cctgcaagag
     8341 gctattaaag aactgatacc agggtttagc aaaatcatca ccgactacac gagccaccgc
     8401 caccgcatat tgaccacctg aaatttctgt cagaatgacg ccctcactgt tttcggggag
     8461 cgtaaagtta ttcggtaccg tcacgacggt gtcgcagcgt aatttttcgg cgggtgtttc
     8521 atccggattg tcgtaataga cagcaaccca ctccttcggc acaatatttt tgctatctac
     8581 ccacatcatc aactgctcaa aacctttctt taccgtctgt tcccacgggc caacgagatg
     8641 gaaacctgca acggtacgtt tatcttcctg cttaatctcg tagttcatga cgcctccatt
     8701 attactgttt ttatatacag tatagttgca atattaaaac cacaaggaat gagtgttgat
     8761 tatgcgagca gactcgcact cctgccagtc tgctgcaaaa gaaaggtcag gccttatggt
     8821 ggaaataatc actcaggcga gaaaacatgc tgccttcccc gacagattcc agggtaacca
     8881 gcggccagtg cgccacctgt ttatcacggt cgtaaagttc aatttcccct acccgctgat
     8941 gggcgctaat tggcgcggtg agctctttac catcaagggt atatttggct ttgatatgtg
     9001 gaatttcggc tttcggtagc accatccaga actcttgttc cgttcccagg gcgatatttt
     9061 ctttatcgcc ataccagatg cgttccgtac cgaccttttt cccacggtgc aaaatttgca
     9121 ccgtagtaaa gttttgttgc ccccaacgca gtaattttct tgcctcttcc tcacgacctt
     9181 ttgcactgtc agcccccatt accactgcaa tgagacgacg ctgcccatct acagccgaag
     9241 caatgagatt aaacccggca ccagaagtat gacccgtttt caggccgtca acattcatgg
     9301 ttttatccca caataacccg ttacggtttt gctgggtgat accgttccag gtgagacttt
     9361 tctcactgta catatgataa aactcgggct cgccgtggat gatagcgcga gaaagcacag
     9421 ctaaatcata agccgagcta tgctgacctg gtgcatccag accatgcact gtttcaaaat
     9481 gcgtatcctt gagatgcagc ttctcggcat agttgttcat catttcaaca aactgccgtt
     9541 gcccaccggc aatatagtca gccagtgcaa cacaagcgtc atttccggaa tccacaatta
     9601 aaccacggct taaatcacgt accgatacgc gatcgccctc tttcaaaaac atcagtgaag
     9661 aaccgacaaa cactggatta tctttcgccc acgcatcgcg ccccacggtg acaatatcgt
     9721 ctggcgtaat gcgatgacta tcgatagcgc gatccacgac atagcccgtc atcagctttg
     9781 tcaggctggc gggattgcgc tgttgatgct cattacccgc ggtgaggatc tgaccggtgg
     9841 tgtaatccat caatacccag gagccggcat gaatctctgg aggctgaggt gaaaaaggaa
     9901 tgttttccgc cgcaaaacca gacgataagt taaaaatgaa caaagaagca gcaataataa
     9961 gacggcgttt caacagcaaa ccctcaggag tttcaaatag ctgttctttt tacggaaata
    10021 cttatgaact ggctggaata aagtgcaaga aaatgtgact accctctcat ttttatctga
    10081 catgatctgt tgccactcgc tgtcaaattg tcgcgctaaa gctgattagc acggtgatat
    10141 ttgatactct ggcagacagc agaaacaacg gatttaacct aatgatgaat gacggtaagc
    10201 aacaatctac ctttttgttt tcacgattac gcgcaacacc cctgagagcc gccatgaaca
    10261 agggtttcat aatttgtgat tttagtttgg tacgcaattt ggtacacaac acaattttca
    10321 cttcagtggg tagtcatcaa actcaccata acgagcatcg ttgatgatgt atgttataac
    10381 cacaaacatc acatctggac tagttccgtt ttcatgagcg ggtatttgtt tacgcctccc
    10441 tttctgcaaa tcctaaagat gagattttgg ggttttggga tatcttttat cctcagttct
    10501 ctatccatta tgcaaaccag taatgaacca tcacagtgtg atagtgaggc gtctgccacg
    10561 ataagcgcag agaaatgatg caatggtggg cggactggct tgatgaaaag gtagagtgag
    10621 cgaccttaaa ctatcgaata gaacaaagtc ttgcaatcca gtgcaaagct ttgtgcgcct
    10681 cagttttgtc taagcgttct actgaaaaca tagtaaaatc ggtaacggct ggaaatcatt
    10741 caatactcgc actatcgaaa gttcgccagc cagccgtggc acgttcttgc atacgacgtg
    10801 ccgcggatcc tattattcta gtatgaaatc cctaaccgat ttcaaattct ccagactgct
    10861 cagattctct tttatcccac gctggcgctg ataaatctcg tcattgcaat cgacctgcgc
    10921 tatcgctgcc gcagttcttc cagtttctgc atcgataact ccatcttctg agcaataaac
    10981 ctcacctatg caatatttta tgtcaattat ttcaattgca ttaatatctt gaatggatta
    11041 catagagtta atgtatccag tactccctat tctcttgcat ataatacatg ttgcaactta
    11101 catctcagcg ctatgaaaaa acaccacctc tctctttatg aaattctgga tttaccaagc
    11161 gctaatttat catttcaatc tacctttaag cattgcattt atctccctac gaggtcatac
    11221 tttaggaagt taaatatgaa tgataatata cctacagcgc gaaatcacaa acaatcgact
    11281 tgtattacag aaaaaacatg cctatctttt taaacttcac agcaggcagt atcttacctg
    11341 agaatgagct agcatcttta cgttatattg tgcagcaaaa tcaaaatgat actgtaatca
    11401 taaaagaacg ttataaaatg gatatccgtt atatcgaatc agtcaatggt tttacagtaa
    11461 atcctgtatg cagtaatcat ttctccatat ttatggcgag acaaaacact attgctcgca
    11521 acctggaaca gcagatcaac aacggacgaa gttttgcaca aatatctcag gattttatgc
    11581 ttcaattatc ttcaaatata ggatggaaaa aaggggccga aaacgccctt aaaaataaaa
    11641 tccattctca ttcatttgtt gtaaatcctg atgaattctc ttgcgacaca caatttctta
    11701 agtgcccaat aacattatgc gttccagaaa aaggggtttt tgtcaagaac gcactgaact
    11761 ccaacatatg cactctttat gataagtctg cgttcatgaa tctcacaaga gaacatctac
    11821 cccaccctct cagcagggaa aagatagtaa aagaaatgat tattgaaagg aatatgtgtt
    11881 attttgacac cataagtcag catttcataa ttatggatac agaccaacag aaacagcatt
    11941 gtaaataaaa tgtaataatt acatactatt agtgattctc atgcatcgta agcggctcgc
    12001 cagaaccgta ttgatattta ctgagcgctc agatcaactt tccatggcaa cagatcgcgt
    12061 acccggtttg ccggccagtc ctggatatgc tcccgccgac aatattaatg gccaggaaat
    12121 gtttaaaaca atgattctgt ttcctaacca tcaagtgtat atgctggctc aattactcaa
    12181 tatctatggg ttgagttcta cccaaagtgg ctctgtagtc gcttcttctt acagtatgtt
    12241 caggtgttgc aggcacagtt gtggagcgta gatgcgttgt tggtttacca tgctctggta
    12301 cataaaaaac gccagtgcca tggtaacctg atatagaaac gttaattccg cgacgttcaa
    12361 actcggcata gacgtgttct gctaaagacc tcttttgacc aaataatgcc ctggccaacc
    12421 agccgttatt catatttgcg gatttttcta tttcatcagg agagaagtct ttgtttttta
    12481 ttatgttggc tgagttacag gatgttaatc tgatatcatc tatttcatga agattatgct
    12541 gaacaatgcg gtcgacaata tctgacggtg ataaaaattc atctccgcac ttaagaagag
    12601 gtagaccagc agaaccatgc ccagacagat aaattttgtt gtattttcct agtgttaaat
    12661 catctggcag gatggttttc atctctgtcg ctgtaatact tatagctgca gcagcaacaa
    12721 catcgctatt acttgattgt agatggcttt tatttgctcc aggatatgta aactccattt
    12781 tccttttatc aaaatcttgt tttgtagcat cattcaggaa taataaatca tacggttttt
    12841 tccccgttgt gactcttgaa acattttggc caagaatatt aagagtatag tcattaacgg
    12901 atttcattcc taaaaaaaca agtaatcctt ctttctcttg cgctttttcg attgttctcc
    12961 ctgctaaagt tattgggatg gattgagatt gttttgtcgg aactgatacg ctggcgctaa
    13021 taggtagtag ggttcttatg ctaaacatac aacctctttc ttaattttcg aacctgttta
    13081 ggattctgtg taaattcaaa ataaacctgt ctgaacattc ctaacaaaca tccaccggac
    13141 atgacaacaa aaaccggagc cggactccgg tttttgtgaa gctgtcggct atttcattcc
    13201 gccaatattt tcccacctcc cgtcagcacg cagaatttgc agcggtctta ccacgcactg
    13261 tatctgcttt ttatccgcat ccagtatcac cacctgcgtg attaccctgt cctgctccgg
    13321 gataat
//
LOCUS       DAZFAB010000118        13322 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZFAB010000118 DAZFAB010000000
VERSION     DAZFAB010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13322
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.118"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            303..1247
                     /locus_tag="ACHJWA_003962"
     CDS             303..1247
                     /locus_tag="ACHJWA_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310466.1"
                     /translation="MSPDNIGSAVNRFSGWPSNDSLKTDKESMTSPVTVQVIKMIAAI
                     LDPHRIVVGIDTLYTTQRETFTSESQQLIESALQTLHVMNLNNLETNRDEESHYLAFE
                     YNVDTSDRITVSAYNEKKPGCYGQCLVIQDDETDLFNLIKIKIFEARHNYVAQLMSDP
                     EFMFKFSYSAQQNRLEPHLLVPAYFPLKTNEPVTQEDVLLLYRFFKMNDDFNKLTSDE
                     YMSILTPLMKCERSVHDNNRYVTGKDTLLLDYPPSGNQIHFHVFPDESATLVLYFSNN
                     DIECFVFERDIPSQYRFFKMFTNLALVIDQLTEANKIL"
     gene            1329..1751
                     /locus_tag="ACHJWA_003963"
     CDS             1329..1751
                     /locus_tag="ACHJWA_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310467.1"
                     /translation="MIPSQISFNTLPVNATYASETTVDTQKFSDILYSAGCSLKDMAY
                     LSRCLASIHPTLAKNIYETENLSDQKLLHIDCRSTNEIKINIFFGQQREGLIEINSDT
                     VIFSILSSLIDSAISKFQHQIPVNGSVNRELLYEDYTD"
     gene            2299..2748
                     /gene="glmS"
                     /locus_tag="ACHJWA_003964"
     CDS             2299..2748
                     /gene="glmS"
                     /locus_tag="ACHJWA_003964"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit S"
                     /protein_id="HFO4310468.1"
                     /translation="MKKATLVIGVIGADCHAVGNKVLDRVFSNHDFRVINLGVMVSQD
                     EYIDAAIETGADAIVVSSIYGHGDIDCLGMRERCIERGLGDILLYVGGNLVVGKHDFA
                     DVETKFKEMGFDRVFAPSHDLEDVCQLMAHDINQRHDVDTRILEEAI"
     gene            2748..4136
                     /gene="glmL"
                     /locus_tag="ACHJWA_003965"
     CDS             2748..4136
                     /gene="glmL"
                     /locus_tag="ACHJWA_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase accessory protein GlmL"
                     /protein_id="HFO4310469.1"
                     /translation="MQIVSVDIGSTWTKAALFTREGDALTLVNHVLTPTTTHHLAKGF
                     FSSLNQVLNVDNALPLLSSGEVALKYSSSAKGGLAVAAMGLVPSITLETAKVTAHSAG
                     AKIAQYYAYKLNRRDIQALEETQPDILLFTGGTDGGEESYGLNNARVLAESKLDCAII
                     YAGNRDIQDEVQEILGHKDLTIVDNVLPDLDHPNPVAARQAICDIFLKRIVKGKGLDV
                     IVDKTGEEPMPTPWTVFELVKAISNVDHSWKEFMLIDMGGATTDVYSACANTLSPDTV
                     LHGVPEPFVKRTVEGDLGMRVSAVVVGESAKELVNVNFAQQPAQLDAFYHYLRHLVAH
                     PDYLPANAEEKYFDTVLAGLCVGYATERHAGTKKEVCTCVGNVDLQMGRDLTTVRKVV
                     GSGGWLSRASQFDMHHWLKYRELNDDGKRILLPTEFEYYRDSRGLLPLLANVARVDPL
                     AAARTSIQCLTL"
     gene            4155..5600
                     /locus_tag="ACHJWA_003966"
     CDS             4155..5600
                     /locus_tag="ACHJWA_003966"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0019670 - anaerobic glutamate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit E"
                     /protein_id="HFO4310470.1"
                     /translation="MELRNKKLTHDEFMTERQQVLKTWETGKDVENFEDGVKYQQTIP
                     EHKRFSLALLKADKEGKTLSQPRAGVALMDEHIELLKTLQEECDLLPSTIDAYTRLNR
                     YEEAAVGIKKSIEAGTSKLNGLPVVNHGVAACRRLTETLQKPLQIRHGTPDARLLAEI
                     SMASGFTSYEGGGISYNIPYAKRVTLEKSIRDWQYCDRLMGMYEEHGIRINREPFGPL
                     TGTLIPPFISHSIAIIEGLLALEQGVKSITVGYGQVGSLTQDVAAIQSLRELAHEYFQ
                     SYGYTDYELSTVFHQWMGGFPEDESKAFAIISWGAAVAGMSGATKVITKSPHEAWGIP
                     TAAANIQGLKASRQMLNMVNEQKFPPCPAVELEIELIKSEVRAVLNKVFELGNGDIAR
                     GTVLAFEAGVLDVPFAPAACNAGKILPVRDNTGAIRVLEAGAVPLPKDILDLHHDYVA
                     ERARCEGRQPTFQMVVDDINAVSHSKLIGRP"
     gene            5600..6841
                     /locus_tag="ACHJWA_003967"
     CDS             5600..6841
                     /locus_tag="ACHJWA_003967"
                     /EC_number="4.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050096 - methylaspartate ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0019670 - anaerobic glutamate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate ammonia-lyase"
                     /protein_id="HFO4310471.1"
                     /translation="MKIKQALFTAGYSSFYFDDQQAIKNGAGHDGFFYTGEPVTQGFN
                     AVRQAGECVSVQLILENGAVAVGDCTAVQYSGAGGRDPLFLAEHFIPFLNDHIKPLLV
                     GRDVDAFLPNARFFDKLRIDGNLLHTAVRYGLSQALLDATALATGRLKTEVVCDEWQL
                     PRVAESIPLFGQSGDDRYIAVDKMILKGIDVLPHALINNVEEKLGFKGEKLREYVRWL
                     SDRILSKRTSARYHPTLHIDVYGTIGLIFDMDPLRCAQYIASLEKEAQGLPLYIEGPV
                     DAGNKPDQIRLLTAITKELTRLGSGVKIVADEWCNTYQDIVDFTDAASCHMVQIKTPD
                     LGSIHNIVDAVLYCNSHSMEAYQGGTCNETDVSARTCVHVALAARPMRMLVKPGMGFD
                     EGLDIVFNEMNRTIALLQAKD"
     gene            6845..8215
                     /locus_tag="ACHJWA_003968"
     CDS             6845..8215
                     /locus_tag="ACHJWA_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HFO4310472.1"
                     /translation="MARSFKVLSPTAILGYGFPEESFRKAMAESPDLIAVDAGSSDPG
                     PHYLGAGKPFTDRAGVKRDLRYMITAGVQNNIPVVIGTAGGSGAAPHLEWCREIIHEI
                     AREEHLSFSMALIPADVDKAIVHQALDNGKITALDFVPPLTHDAIDESTYIVAQMGIE
                     PFQRALKERAQVVLGGRAYDPACFAALPIMQGFDEGLALHCGKILECAAIAATPGSGS
                     DCAMGIIDDNGFTLKTFNPKRKFTETSAAAHTLYEKSDPYFLPGPGGVLNLKGCTFKA
                     VNDGEVYVSGSKHEETPYALKLEGARRVGFRCLTIAGTRDPIMIAGIDKIIDEVKTSV
                     SRNLSLDDDSIRINFHLYGKNGVMGDHEPMQTAGHELGIVLDVVAPTQEIANSVCSLV
                     RSTMLHYGYENRIATAGNLAFPFSPSDIQGGPVYEFSIYHLIEANDALRFDFHIEQVT
                     PEGVQA"
     gene            8212..8529
                     /locus_tag="ACHJWA_003969"
     CDS             8212..8529
                     /locus_tag="ACHJWA_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4387 domain-containing protein"
                     /protein_id="HFO4310473.1"
                     /translation="MKQSIVSLAQVIRSKNAGPYELVLDILFKTKENYERVKSSGQLT
                     PELIARLYHVEPDFIHRIVWFDPSNAVKIVMPRDIISGNVGDNDVYGAQQHAPLLNMS
                     FDL"
     gene            8690..9970
                     /locus_tag="ACHJWA_003970"
     CDS             8690..9970
                     /locus_tag="ACHJWA_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308785.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HFO4310474.1"
                     /translation="MKKISLTTMILLALVLGMIIGVVLNNTASPETAKLYAQEISIFT
                     TIFLRLIKMIIAPLVVSTLVVGIAKMGDAKALGRIFSKTLFLFICASLLSIALGLITV
                     NFFMPGTGINFVAHGAETTGVVAAEPFTLKVFISHAFPTSIVDAMAHNEILQIVVFSI
                     FLGCSLTAIGEKGSAIVHALDSLAHAMLKLTGYVMLFAPLTVFAAISALIAERGLAVM
                     VSAGIFMGEFYFTMLLLWVLLIGLAIVYVGPCIRRLTRALSEPALLAFTTSSSEAAFP
                     GTLEKLEQFGVSPKIASFVLPIGYSFNLVGSMAYCSFATVFIAQACNIHLSIGEQITM
                     LLILMLTSKGMAGVPRASMVVIAATLNQFNIPEAGLILLMGVDPFLDMGRSATNVMSN
                     AMGAAMVSRWEGEHFGEGCRGKALKPNESNVALP"
     gene            10078..11730
                     /locus_tag="ACHJWA_003971"
     CDS             10078..11730
                     /locus_tag="ACHJWA_003971"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO4310475.1"
                     /translation="MSKPFIWQEPFLQNKDGTEYTLISDQHITVTELDGEEVIKIAPE
                     ALTLLARQAFYEASFFLRSAHLQQVASILNDQQASSNDKYVALQLLRNAEVSAKGVLP
                     NCQDTGTATIVASKGQQIWTGGNDAEALSKGIYSTFQENNLRFSQNAPLDMYTEVNTQ
                     TNLPAQIDISAVAGDEYHFLCVNKGGGSANKAALYQETKSLLQPEKLTAFLIEKMKSL
                     GTAACPPYHIAFVVGGLSADQTLKIAKLASTKYYDNLPTSGNEQGQAFRDIELEKVLL
                     EASQQFGIGAQFGGKYFAHDIRVIRLPRHGGSCPIAMALSCSADRNIKAKINKHGIWL
                     EKLEHNPGQYIPASLREENHAQHVQLDLNRPLRDVMQDLARLPVGTRVSLSGPIVVAR
                     DIAHAKIKARLDSGEPMPEYLKHHIVYYAGPAKTPENMACGSLGPTTGGRMDGYIDTF
                     QAAGGSLVMLSKGNRSQQVTDACHKHGGFNLGSIGGAAALLAQEYVKSLRCLEYPELG
                     MEAVWMMEVENLPAFILVDDKGNNFFSQFEQQHRCASCPAGH"
     gene            11740..12342
                     /gene="cobO"
                     /locus_tag="ACHJWA_003972"
     CDS             11740..12342
                     /gene="cobO"
                     /locus_tag="ACHJWA_003972"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308783.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFO4310476.1"
                     /translation="MEARISTERHVQRQQKLKEQVDTRVAAAIEKKGILIVFTGNGKG
                     KSTAAFGTVTRAVGHGKTVGVAQYIKGQWDNGEYNLLQPLGVEFHIMGTGFTWETQNR
                     QADIDAAKEVWSESKRMLADKRYDLVVLDELTYMLAYHYLDTEEVIASLQNRPAQQSV
                     IVTGRGCHSQILKMADTVSEIRPVKHAFDNGIQAQPGIDW"
     gene            complement(12376..13281)
                     /locus_tag="ACHJWA_003973"
     CDS             complement(12376..13281)
                     /locus_tag="ACHJWA_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO4310477.1"
                     /translation="MRGKIPKTELLVTFEVVARHESYTRAAEELALTQSAVFRQVSAL
                     EEFLRTPLFSHSKKRIFLNDTGKYYLGIVKETLNKLERDTNTIMTWQPTVQVIELAVN
                     PTFSTHWLIPNLHEFTKLHPDIIVNIHSLANNGDFLNREYDAVIMRENFCAPWAEVEY
                     LFEEEILPVCSGSLLAMSDQKLSVAELLTELPLLHQSTRITGWEEWFALSGVSSPLVN
                     NGPRFDLLSMLIAAVRSNLGVALLPRFAIQHDLDSGDMVIPCDVPIRTGNRFIMTWQE
                     EKSDSPHLQQFREWLLAKSVVPQEM"
BASE COUNT         3609 a         3241 c         3160 g         3312 t
ORIGIN      
        1 ttttataata ccaattgatt agagtagcca atttgttgct attttacata acatattttt
       61 atcaaagtta attgattgtt gcctttatga gcaccgaccg taagcccgct atgttattgt
      121 ttcattagta ataatcactt aaatgtatcg atgaaacagg tatgacttac cgggagtgat
      181 tgtttctgag tgaacagagg ccactttcgc ctgataaatg aaaaatgagc gatttttgca
      241 agttagtttt tctatagccg agtacgctca gcgcctcgct tgattacaat gaggttgctg
      301 ctatgagtcc tgataatatc ggttcggctg tcaatcgctt ctcaggttgg ccatctaatg
      361 attctctaaa aacagataaa gaatcaatga catcacccgt gactgtgcag gtaataaaga
      421 tgattgccgc catccttgac ccacatcgga tagtcgttgg aattgataca ctttatacaa
      481 cgcagcgcga aacatttacc tcagagagtc aacagctcat tgaatcagca ttgcaaacac
      541 ttcatgtgat gaatttaaat aatctggaaa caaatcgcga tgaagaaagt cactatctgg
      601 cttttgaata taacgttgac acctctgaca gaataaccgt ttctgcttat aatgaaaaaa
      661 aacctggctg ctatggccaa tgtcttgtaa tacaggatga cgaaacagac ttatttaacc
      721 tgataaaaat taaaattttc gaagccaggc ataattatgt cgctcaattg atgagcgatc
      781 cggaatttat gttcaaattc agttatagcg ctcaacaaaa tagattagaa cctcatcttc
      841 tggttcccgc ttatttccct ctaaaaacaa acgaaccggt tacgcaagag gatgtactgt
      901 tactttatcg tttctttaaa atgaatgatg attttaataa attaacatca gatgaataca
      961 tgagtatcct gacacctctg atgaagtgtg agcgtagcgt ccacgacaac aacaggtatg
     1021 tgacgggcaa agataccctt cttctggact atcctccttc tggtaaccag atacatttcc
     1081 atgttttccc tgacgaatca gcaacactag tcctttattt ttcaaacaat gacattgaat
     1141 gtttcgtctt tgaaagagat attccgagcc agtatcgctt tttcaaaatg tttaccaatc
     1201 tggcactggt tatagatcag ttaaccgaag ccaataaaat actttaattt tctatatatc
     1261 ccctatggca aatattgcta aacaatgcct ggggattaat ttttattact cacggaaaga
     1321 tttaaataat gataccatct cagatctctt tcaataccct tccagtcaat gcaacatacg
     1381 cttcagagac aacagttgac acacaaaaat tctctgacat cctctactca gcggggtgtt
     1441 ccctaaagga tatggcttat ctgagcagat gtctggcatc aatacacccg actctcgcca
     1501 aaaacattta tgaaacagaa aatctcagcg atcaaaaatt actacatatt gattgccgta
     1561 gtacaaatga aatcaagata aatatctttt ttggtcaaca acgagaagga ttaattgaaa
     1621 ttaattctga cacagtcatt ttttcgatat tgtcttcatt aattgactcc gccatttcta
     1681 aatttcaaca ccaaatcccc gttaacggtt cagttaatcg tgaactattg tatgaggatt
     1741 atactgatta aatccaccct gtcgcgaaaa atggcctgcg ttattgccag acgtaataaa
     1801 acctttgatt aacgttactc atactgatag caccttcatg tacctctctt ttttacgaaa
     1861 aattaaaaga gctaactaag gtcataattc gcatgaatca ttaaccattt gttattttca
     1921 acattatttt tacattaagc agagatgaat tgcagtgaaa ttcgacttaa atcttatgaa
     1981 atgcatttac tcatggctaa aaccacgtca caaaaatgca aattagaagt agatcacaat
     2041 tatctcaata cattaaatca agttaatttt aaaaatttaa cactcatgac attagttgac
     2101 ataacacact gaattaacat ggaaatttaa aaacacactt caaacaatca tttcccgaaa
     2161 gacattttga aaatagatca attgcaacat tacggtcaga taaagacact cctgtgatat
     2221 taaacctgtg ccgttatgaa cggccataaa tacgacgtgt gtaacttatt ttactctctg
     2281 tcttacaaag gattacccat gaagaaagca acacttgtga ttggcgttat tggcgcggac
     2341 tgccatgcag taggcaataa agttctggac cgcgttttta gcaaccacga cttcagagtc
     2401 attaatctgg gcgtaatggt cagccaggac gaatatattg atgctgccat tgaaactggc
     2461 gctgacgcga ttgtcgtctc ctctatctat gggcatggcg atatcgactg tctgggcatg
     2521 cgcgaacgct gcatcgagcg tggactgggg gacatcctgc tctatgtggg cggtaacctg
     2581 gtggtcggaa aacatgactt cgctgacgtg gaaaccaaat tcaaagagat gggatttgat
     2641 cgcgtctttg ccccaagtca tgacttagaa gatgtttgcc agctgatggc ccacgatatc
     2701 aaccaacgtc atgacgttga tactcgtatt cttgaagagg ctatctgatg caaatcgtct
     2761 ctgtcgatat tggatcgacg tggaccaaag cagccctctt tacccgggag ggggacgcgt
     2821 taacgttggt taaccacgta ctgaccccaa ccaccaccca tcatctggcg aaggggtttt
     2881 tctccagcct gaaccaggtg ttaaacgtag ataatgccct ccccctgtta agcagcggtg
     2941 aggttgcatt gaagtattcc tcctctgcca aaggtgggct ggcggttgcc gcaatgggat
     3001 tggtgccttc tatcaccctg gaaaccgcga aagtaaccgc tcactctgcg ggtgctaaaa
     3061 ttgcgcaata ttacgcctac aagctgaacc gccgtgatat ccaggcactg gaagaaacgc
     3121 agcccgatat tctcttgttc accggcggca ccgacggcgg agaagaaagc tacggactga
     3181 ataacgcccg tgtgctggca gaatccaaac tcgactgcgc cattatttat gccggaaacc
     3241 gggatattca ggacgaggtg caggagatcc tcgggcataa agacctgacc attgtcgaca
     3301 acgttctgcc cgatcttgac catccgaacc ctgtagctgc acgccaggct atttgcgaca
     3361 tattccttaa acgcatcgtt aaagggaaag ggctggatgt cattgttgat aaaacaggcg
     3421 aagaaccaat gccgacacca tggacggtct ttgaactggt caaggcaatc agtaacgtcg
     3481 atcacagctg gaaagagttc atgctgatcg acatgggcgg cgcaaccacc gatgtttatt
     3541 ccgcctgtgc caacacgctc tcgcccgaca ccgttttaca cggcgtaccg gaacccttcg
     3601 taaaacggac ggtggaaggc gatctgggaa tgcgcgtctc ggcagtggtg gttggtgaaa
     3661 gtgcgaaaga gttagtcaat gtgaattttg ctcagcaacc cgcacagctg gacgcttttt
     3721 atcattacct ccgtcatcta gtcgctcatc ccgactactt acccgcaaat gcagaagaga
     3781 aatatttcga taccgtcctg gccggactgt gtgtgggtta tgccaccgaa agacatgcgg
     3841 gcaccaaaaa agaggtctgc acctgcgtcg gcaatgtcga tttacagatg ggacgtgacc
     3901 tgacaaccgt gcgtaaagtc gtcggatccg gcggatggct ctcgcgtgcc agtcagttcg
     3961 atatgcatca ctggttgaaa taccgcgagt taaacgacga cggtaaacgc attctcttac
     4021 cgaccgaatt tgaatattac cgcgattccc gggggctatt accactgctg gcaaacgtcg
     4081 ccagagtcga tccgctggcc gccgcgcgca ccagtattca atgcttaacc ctataacaat
     4141 taaggctgca atcaatggaa cttcgaaata agaaattaac ccatgacgaa ttcatgaccg
     4201 agcggcaaca ggtactgaaa acctgggaaa ccggcaagga tgttgaaaac tttgaagatg
     4261 gcgttaaata ccaacaaacc atccctgagc ataaacgttt ctctcttgcg ctgctgaaag
     4321 cagacaagga aggtaaaacc cttagccaac cgcgcgcagg tgtggcgctg atggacgaac
     4381 acatcgaact gctgaaaaca ctacaagaag agtgcgatct gctgccaagc accatcgatg
     4441 catatacccg tctgaatcgg tacgaagaag cggctgtagg gattaaaaaa tccatcgaag
     4501 ccggaacctc taaactgaac ggtttaccgg tagttaacca tggtgtcgca gcttgccgtc
     4561 gcctcaccga aaccctgcaa aaaccgctac agatccgcca cggcacgcca gatgcgcgtc
     4621 tgctggctga aatctcaatg gccagcggct ttaccagtta tgaaggcggt ggcatttcct
     4681 acaacattcc gtatgccaaa cgcgtaacgc tggaaaaatc gatccgcgac tggcagtact
     4741 gcgaccgttt aatggggatg tatgaagaac acgggatccg catcaaccgc gaaccgtttg
     4801 gcccactaac gggtacgctg atcccgccgt ttatctcgca ctctatcgcg ataatcgaag
     4861 gcttgctggc gctggaacag ggtgtgaaat ctatcaccgt tggttatggc caggtcggca
     4921 gcctgacgca ggatgttgcg gcgatccagt ctctgcgtga acttgcacac gaatattttc
     4981 agagctatgg ctataccgac tatgaattga gtaccgtgtt tcaccagtgg atgggcggct
     5041 ttccggaaga cgaatcgaaa gcgttcgcta ttatctcctg gggcgcagcc gtcgcgggga
     5101 tgtctggtgc caccaaagtc atcaccaaaa gtccgcacga agcctggggt atcccgactg
     5161 ccgctgccaa cattcagggg ctgaaagcct cccgccaaat gctcaacatg gtcaatgagc
     5221 agaaattccc gccgtgccct gcggttgaac tggaaattga actgattaag agcgaggttc
     5281 gcgcggtact gaataaagtg ttcgaactgg gtaatggtga tattgcgcgc ggaacggtgc
     5341 tggcatttga agcgggcgta ctggatgtgc cgtttgcgcc agccgcctgc aacgcgggca
     5401 aaattcttcc ggtccgtgac aacaccggcg cgatacgcgt actggaagcc ggagccgttc
     5461 cattgccaaa agatattctc gacctccacc acgattacgt tgcggaacgc gcacgctgtg
     5521 aaggacgcca accgacattc cagatggttg tagatgacat caatgctgta tcccacagta
     5581 aattaatagg aagaccataa tgaaaataaa acaggctctg ttcaccgctg gctactcctc
     5641 attctatttc gatgaccagc aggcgataaa aaacggagcg ggtcatgacg gcttttttta
     5701 taccggggag ccagtaacac aggggtttaa cgccgtacgt caggccgggg agtgcgtatc
     5761 ggtacagttg attctggaaa acggcgcggt cgccgtaggt gactgtactg ccgtacagta
     5821 ttccggggca ggcggtcgcg atccactgtt cctcgcagag cactttattc cgttcctcaa
     5881 cgaccatatc aagccattac tggtaggccg cgatgtggat gctttcctgc cgaatgcccg
     5941 tttcttcgac aaattgcgta ttgacggcaa cttgctgcat accgccgtgc gctacggatt
     6001 atcacaggcg ctgcttgatg ctaccgcgct ggcaaccggc cgtctgaaaa ctgaagtggt
     6061 ctgtgatgaa tggcagttgc cacgcgtggc ggaatccatt ccattatttg gtcagagcgg
     6121 cgacgatcga tatatcgccg tcgataagat gatccttaaa ggcatcgacg tgctgcccca
     6181 tgcgctgatt aataacgtcg aagagaagct gggctttaaa ggtgaaaaac tgcgcgaata
     6241 tgtccgctgg ttgtcggatc gcattctaag caagcgcacc agcgcacgct accaccctac
     6301 cctgcacatc gatgtatacg gcactatcgg tctgatcttc gatatggatc cgcttcgctg
     6361 tgcgcaatac atcgccagcc tggaaaaaga agcgcaaggc ctgccgctct acatcgaagg
     6421 gccggtcgat gccggtaaca agcccgatca aattcgcctg ctgaccgcga ttaccaaaga
     6481 gctgacgcgc ctcggttccg gcgtgaaaat tgtggccgat gaatggtgta acacctacca
     6541 ggatattgtt gatttcactg atgctgccag ttgtcacatg gtgcaaatca aaaccccgga
     6601 tctgggcagt attcacaaca tcgtcgatgc ggttctttac tgcaacagcc acagcatgga
     6661 agcgtaccag ggcggcacct gcaatgaaac tgatgtcagt gcccgcacct gtgtccacgt
     6721 cgcccttgcc gctcgcccca tgcgtatgct ggtaaaacca gggatgggct ttgacgaagg
     6781 cctcgatatc gtcttcaacg aaatgaatcg tactatcgcg ctgttgcagg ctaaggatta
     6841 acaaatggct cgttctttta aagtcttatc gccaacggca atcctcgggt atggattccc
     6901 ggaagagagc ttccgcaaag cgatggcaga atcgcccgat ctgatcgccg ttgatgcagg
     6961 ctcttccgat ccaggccccc actacctcgg ggcaggcaaa ccgtttaccg atcgcgcagg
     7021 ggtcaagcgc gatctgcgct atatgatcac cgcaggcgtg caaaataata taccggtggt
     7081 gatcggaacg gctggcggtt ctggtgccgc accacacctg gagtggtgcc gagaaataat
     7141 tcatgagatt gcccgggaag agcacctctc gttctcgatg gcgttgatcc cggcagacgt
     7201 tgacaaagcc atcgtccacc aggcgctgga taacggcaaa atcacggcgc tggattttgt
     7261 cccgccgtta acccacgacg cgattgacga aagtacgtat atcgtcgcgc aaatgggcat
     7321 cgaacccttc cagcgggcgc tgaaagaacg cgcgcaagtg gtgctgggcg ggcgcgctta
     7381 cgacccggcc tgctttgctg cgctcccgat catgcaaggc tttgatgaag gtctggcgct
     7441 gcattgtggg aaaatccttg aatgcgcggc aatcgccgca acgcccggct caggctctga
     7501 ctgtgcgatg ggcatcattg atgacaacgg ctttacgctg aaaacattta atccgaagcg
     7561 taaatttacc gaaacgtcag cagctgcaca cacactgtat gagaaatccg atccctactt
     7621 cctgccagga cctggcggcg tgttgaacct gaaaggctgc acatttaaag cagtaaatga
     7681 cggcgaagtc tacgtcagcg gttctaagca tgaagaaacg ccgtatgccc tgaaactgga
     7741 aggtgcacga cgggtgggct tccgctgtct gaccatcgcc ggaacgcgcg acccgattat
     7801 gatcgccggg atcgataaaa tcatcgatga agtcaaaacc agcgtttcgc gtaacctgtc
     7861 gctcgacgat gacagcattc gcatcaattt ccacctgtac ggcaaaaacg gggtgatggg
     7921 agaccatgaa ccgatgcaaa ctgccgggca tgagctgggg attgtccttg atgtagttgc
     7981 accgacccag gaaattgcca acagcgtttg ctcgctggtg cgctctacca tgctgcacta
     8041 cggctatgaa aaccgcatcg ctaccgcagg taatctggcg ttcccgttct ccccttctga
     8101 tatccagggc gggccggtat acgaattttc catatatcac ctgattgaag ccaacgacgc
     8161 tctgcgtttt gatttccata ttgaacaggt gacgccagaa ggagttcagg catgaaacag
     8221 tcaattgtct cactggcgca agtcattcgt tcgaaaaatg ccggacctta cgaactggtt
     8281 ttagatattt tatttaaaac gaaagaaaac tatgagcgag ttaaatcatc agggcaatta
     8341 accccagaac tgattgcacg cttatatcat gttgaacctg actttattca tcgtattgtc
     8401 tggtttgatc cttctaacgc agtaaaaatc gtcatgccac gagatattat ctccgggaat
     8461 gtaggtgaca atgatgtcta tggtgcacag caacatgcac ctttattaaa tatgagtttc
     8521 gatttgtaaa aatccgaata cttttagcca actgacatcc cgtcacttat tgcagttcaa
     8581 cacttcagct atttgtaagg ttgtaatagc aggtagatgc ctgaatctct attcaggcat
     8641 ctacttctcc tgattctgat ttatcgccgt gcacctgatg gaggaaaaaa tgaagaaaat
     8701 aagtttaacc acgatgattc ttttggcgct ggtacttgga atgattatcg gcgtagtgct
     8761 caataacact gcttcaccgg aaaccgcaaa actctatgcg caagaaatat cgatattcac
     8821 gacgattttc ttacgactga taaaaatgat tatcgctccg ttagtggtct ctaccctggt
     8881 ggtaggtatt gctaaaatgg gcgatgccaa agcccttggt cgtatttttt ctaaaacact
     8941 ctttttattt atttgcgcct cattgctgtc aattgcctta ggcttgataa cggtaaattt
     9001 cttcatgcca ggtacaggaa ttaattttgt tgcacacggt gccgaaacca ccggagtggt
     9061 cgcggcagaa ccctttacgc taaaagtatt tatttcgcat gctttcccca ccagcattgt
     9121 cgatgccatg gcgcacaatg aaattttgca aatcgtggtg ttctcaattt tcctcggctg
     9181 tagcctgacg gcgattggcg agaaaggcag cgccatcgtt cacgccttag attcgctggc
     9241 acatgccatg ttaaagctca ctggctacgt catgctcttc gctcccctga ccgtattcgc
     9301 tgctatttca gcattgattg ctgaacgagg actggcagtt atggtgagcg ccgggatctt
     9361 tatgggtgaa ttttatttca ccatgttatt actttgggta ctgcttatcg gtctggccat
     9421 cgtttatgtc ggcccctgca tcagacgcct gacccgcgcc ctttcggaac ccgccctgct
     9481 ggcatttacc acatccagtt ctgaagcggc ttttccggga acgcttgaaa aactggagca
     9541 atttggcgtt tcccccaaaa ttgccagctt tgtcttaccc attggctact catttaatct
     9601 cgttggatca atggcctact gctccttcgc cacagttttc atcgcccagg cctgcaatat
     9661 ccatttatcc atcggtgagc aaatcaccat gctgttgatc ctgatgttga cctcgaaagg
     9721 aatggctggc gtaccacgcg cctcaatggt ggttatcgcc gccacgctca accagttcaa
     9781 tattccggaa gcggggctga tcttgctgat gggcgttgat ccgttccttg atatggggcg
     9841 ttccgcgaca aacgtcatga gcaacgcaat gggcgctgcg atggtgagtc ggtgggaagg
     9901 cgaacatttc ggcgagggct gtcggggtaa agcattaaaa cccaatgaat cgaacgttgc
     9961 tctgccctga gggggatctt aagcaatcag aagccaatga ctacgcattt ttataagccg
    10021 gatcatgaag ttagacggca attaagtaac actaacgttt aaggtgaaaa gtgaaatatg
    10081 tccaaaccat ttatctggca agaaccgttt ctacaaaata aagacggtac agaatataca
    10141 ttaattagcg atcaacatat tacagtaacg gaattagacg gcgaagaagt gataaaaatc
    10201 gctcctgaag cattaacgct gcttgcccgg caggcatttt atgaagcctc ttttttctta
    10261 cgttccgctc atttgcaaca ggtcgccagc attcttaacg atcagcaggc cagcagtaat
    10321 gataaatacg ttgcgttgca gttattacgt aatgctgaag tctcagcaaa aggggtatta
    10381 cccaactgtc aggataccgg caccgcaact attgttgcca gtaaagggca acagatatgg
    10441 accggaggta acgacgccga agccttaagt aaaggcattt acagtacttt ccaggaaaat
    10501 aatctgcgct tttcacaaaa tgcgccgctg gatatgtata ccgaagtcaa tacgcaaacc
    10561 aatctcccgg cgcagattga tatcagcgct gtcgctggtg atgagtacca ttttctctgt
    10621 gttaataaag ggggcggctc cgccaataaa gccgcgcttt atcaggaaac caaatcctta
    10681 ttgcaaccag aaaaactgac cgcattttta atcgaaaaaa tgaaatcgct gggcaccgct
    10741 gcctgcccgc cttaccacat cgcctttgtc gtcggagggc tttcagccga tcaaacctta
    10801 aaaattgcca agctggcctc aacgaaatat tacgacaatt tgcccaccag cggaaacgag
    10861 cagggacaag cattccgtga tattgagctg gaaaaagtgt tgctcgaggc cagtcagcaa
    10921 tttggtattg gtgcgcaatt tgggggtaaa tatttcgctc atgatattcg cgtcattcgt
    10981 ctgccgcgtc acggtggctc ttgcccgata gctatggcgc tctcctgttc tgccgatcgc
    11041 aatatcaaag caaaaattaa caaacacggt atctggctgg aaaaactcga acacaatccg
    11101 gggcaatata ttccagcctc actgcgggaa gaaaatcacg cgcaacatgt acaactggat
    11161 ctaaaccgtc cgttgcgtga cgtgatgcag gatctcgcca gattaccagt aggcacccgc
    11221 gtgtcgttaa gtggtcccat cgtagtggcg cgcgatatcg cccacgccaa aataaaagcg
    11281 cggctggata gcggcgaacc gatgccggag tacctgaagc atcatattgt ctattacgct
    11341 ggcccggcga aaacaccgga gaacatggcc tgcggttcac tgggtccaac cacgggcggt
    11401 cgaatggacg gttatatcga taccttccag gcggctggcg gcagtctagt gatgttatcg
    11461 aaaggtaacc gcagccagca ggtcaccgat gcctgtcaca aacatggcgg cttcaatctg
    11521 ggaagtattg gcggtgcagc tgcgttactg gcgcaagaat atgtcaaaag tctgcgttgc
    11581 ctggaatacc cggagctggg tatggaagcg gtgtggatga tggaagtgga aaatctgccc
    11641 gcattcatcc tggtagatga taaaggcaat aatttcttta gtcagtttga gcagcaacac
    11701 cgctgcgcca gttgccctgc gggacattaa ggaaaatgaa tggaagcgcg aatttcaact
    11761 gaacggcatg tgcagcgcca acagaaattg aaggaacagg tcgatacgcg cgtcgcggcg
    11821 gcgatagaga aaaaagggat cttgattgtt ttcaccggca acggcaaagg gaaatccact
    11881 gccgcgtttg gtactgtgac gcgcgcggtg ggtcatggca aaacggtggg ggttgcgcag
    11941 tacatcaagg ggcaatggga caacggcgaa tacaaccttc tccagccgct gggggttgag
    12001 tttcatatta tgggaaccgg ttttacctgg gagacacaga accggcaagc ggatatcgac
    12061 gcggcgaaag aggtctggtc ggaaagcaaa cggatgctgg ctgacaagcg ttacgactta
    12121 gtggtgcttg atgagctgac ctatatgctg gcttaccatt atctggatac cgaagaagtg
    12181 atcgcttctc tgcaaaaccg cccggcacaa caaagtgtca ttgtcaccgg acgcggttgc
    12241 cacagtcaga tattaaaaat ggcagatacg gtcagcgaaa tccgccccgt caaacatgct
    12301 tttgataacg gcattcaggc ccagccgggt atcgactggt aataactgcg cctggcatcc
    12361 cgccaggcgc aggttttaca tctcctgcgg tacgaccgat ttcgccagca accattcacg
    12421 aaattgctgc aagtgcggtg agtcagactt ctcttcctgc cacgtcataa taaaacgatt
    12481 accggtacga ataggcacat cacagggaat caccatatcg ccactatcca aatcatgttg
    12541 aatagcgaaa cgtggtaaca gcgccacgcc gagattagag cgcacggcgg caatcagcat
    12601 tgaaagcaaa tcgaaacgtg gtccgttgtt gaccagcgga ctgctaacgc cagataaagc
    12661 aaaccactct tcccagccag taatacgggt gctttgatgc agaagtggta actcagtaag
    12721 cagttctgcc actgacagtt tttgatcgga cattgcaagc aaactgccgc tgcataccgg
    12781 caaaatctcc tcttcaaaga gatattctac ttccgcccag ggcgcgcaaa aattttcgcg
    12841 cataatgaca gcatcgtact cccgattaag aaaatcgccg ttattagcta aagaatgaat
    12901 attaacaata atatcgggat gtaactttgt gaattcatgt aaatttggga ttaaccagtg
    12961 ggtactaaaa gtaggattaa cggcaagttc aataacctga accgtaggct gccaggtcat
    13021 aatggtattg gtatcgcgtt ccagcttatt taatgtttct tttactatcc ccaggtaata
    13081 cttccctgta tcattaagaa aaatgcgttt tttagagtgt gaaaataacg gagtacgtaa
    13141 aaattcctcc agcgcactta cttgtctgaa tactgcactt tgggttaacg ctaactcctc
    13201 tgccgctctg gtataacttt catgacgtgc gacgacctca aaagtcacca gcagttccgt
    13261 tttaggtatt ttccctcgca taatgccgtc ccttaaaaat agtttaaacc atcaaaaata
    13321 ca
//
LOCUS       DAZFAB010000119        12823 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZFAB010000119 DAZFAB010000000
VERSION     DAZFAB010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12823
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.119"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(11..1423)
                     /locus_tag="ACHJWA_003974"
     CDS             complement(11..1423)
                     /locus_tag="ACHJWA_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310478.1"
                     /translation="MSYALIDNASLTAVERTLGDILVKNPDTINGDLVAFENLIQAIL
                     FYDTLICVDNYKKEYRDKRIAKFDFIKFVSESDFQLSELDQLAQVESRQITSEIRGGE
                     FVDDDFRQLVEMLKLNMICTWDLRSSVYYLTMKMLGQPGTPEYAKYSELSASIFNELS
                     DASDTKGYWSTDVKLVSSSGHEYTEREFELEREKSTRGKGGMTRSLEMFIASLNWLAY
                     KSIYYSVIAKHFKADSFIHPIRHAYQLHWMKKTGAFGHDYTARLVQSLSDKISTARSE
                     IVDHGRTSTISLDLPIFSAWLANETGNVSQVINSALELRTHDHFRTCRDVIREIHVTY
                     DESGISAGNKKVTKLLSDLDKISGDIKRSYGVPSNQGIQGSFSIKCINSLTGLVGIPG
                     LPDKEFALSTPEFMKSKQHKAFSTVFKDVTNELTSIERLGGLRDKLASNFTIDDSYYT
                     EPKIEDPRFRYVSSNWKQPM"
     gene            complement(1610..1786)
                     /locus_tag="ACHJWA_003975"
     CDS             complement(1610..1786)
                     /locus_tag="ACHJWA_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310479.1"
                     /translation="MEKDLKELREYLLLSPVYSEINDCLASLSMEDVTNEEFAERLGI
                     APDWMDAIDTSQLA"
     gene            2089..2715
                     /locus_tag="ACHJWA_003976"
     CDS             2089..2715
                     /locus_tag="ACHJWA_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO4310480.1"
                     /translation="MNVRIYCRASTEGQHADRALTSLREFSKSKGWQIAGEYIENASG
                     AKLERVELMRLLSEAQSGDLLLVEAIDRLSRLEHSAWVELKDTLNRKGLIIVSMDLPT
                     SWQMVEMAGNDLTSGILRAVNAMLIDILATMARQDYETRRKRQQQGIERAKSEGIYIG
                     RAKNQEAREIVREMLEQGVKPELIMKAAGISRATYYRIKNELLIVKSE"
     gene            3066..3233
                     /locus_tag="ACHJWA_003977"
     CDS             3066..3233
                     /locus_tag="ACHJWA_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001316510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO4310481.1"
                     /translation="MLHSAMHSLMNSQARDTRDRSQNDDNVLEISEPCIRSTFERLYQ
                     NMNEVFGLGDY"
     gene            complement(3313..4560)
                     /locus_tag="ACHJWA_003978"
     CDS             complement(3313..4560)
                     /locus_tag="ACHJWA_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HFO4310482.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(4632..5546)
                     /locus_tag="ACHJWA_003979"
     CDS             complement(4632..5546)
                     /locus_tag="ACHJWA_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HFO4310483.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGT
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTEIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDRDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVDCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            5762..7195
                     /gene="cscA"
                     /locus_tag="ACHJWA_003980"
     CDS             5762..7195
                     /gene="cscA"
                     /locus_tag="ACHJWA_003980"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFO4310484.1"
                     /translation="MTQSRLHAAQNALAKLHERRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDENDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFVAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSISPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVVQHGALWQLG"
     gene            complement(7203..8198)
                     /locus_tag="ACHJWA_003981"
     CDS             complement(7203..8198)
                     /locus_tag="ACHJWA_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO4310485.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNDHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGSVTRIPCPLLIRCST"
     gene            8441..8659
                     /locus_tag="ACHJWA_003982"
                     /pseudo
     CDS             8441..8659
                     /locus_tag="ACHJWA_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            8677..10005
                     /gene="dsdA"
                     /locus_tag="ACHJWA_003983"
     CDS             8677..10005
                     /gene="dsdA"
                     /locus_tag="ACHJWA_003983"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFO4310486.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLEDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAEQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMEQYLAKGR"
     gene            complement(10113..11651)
                     /gene="emrY"
                     /locus_tag="ACHJWA_003984"
     CDS             complement(10113..11651)
                     /gene="emrY"
                     /locus_tag="ACHJWA_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFO4310487.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     SINLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVIFLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTETIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(11651..12814)
                     /gene="emrK"
                     /locus_tag="ACHJWA_003985"
     CDS             complement(11651..12814)
                     /gene="emrK"
                     /locus_tag="ACHJWA_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFO4310488.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
BASE COUNT         3715 a         3013 c         2787 g         3308 t
ORIGIN      
        1 ttaactatat ctacattggc tgcttccaat tactacttac gtaacggaat ctcggatcct
       61 ctatttttgg ctcggtataa taagaatcat caatagtaaa gttcgacgct aatttatccc
      121 gaagcccacc taaacgttca atgcttgtta gttcgttagt aacatccttg aaaacggtag
      181 aaaatgcttt atgttgttta gacttcataa attcaggagt gctcagtgca aactctttat
      241 caggcaaacc tggaataccc acgagtcctg tcaatgaatt tatacatttt atcgaaaaac
      301 tgccttgaat accttgattt gatggaacac cgtagcttcg tttaatatct ccagatattt
      361 tatctaaatc acttagtaat tttgttactt ttttatttcc tgcacttatc cccgattcat
      421 cgtaggtcac atgaatctct cggattacat ctcgacacgt acgaaaatga tcatgtgttc
      481 taagctctaa agctgaattg ataacttgag agacatttcc tgtttcgttt gctagccatg
      541 cagaaaatat tggtaaatct aatgaaatag tagatgtgcg tccatgatct acaatttcag
      601 atcttgcagt actgatttta tcagacaacg attgcaccaa ccttgcagta taatcatgac
      661 caaaagcacc tgtttttttc atccaatgca attgatatgc atgccttatt ggatgaataa
      721 atgagtctgc tttaaaatgc ttagcaatga cgctgtaata aatagattta tatgccaacc
      781 aatttagcga tgcgataaac atttcaagtg aacgagtcat acctccttta ccgcgagtag
      841 atttttccct ttctagttca aattcacgct cagtatattc atgtccagat gaactcacta
      901 gcttcacatc ggttgaccag taccctttcg tatcagaagc atctgataat tcattaaaaa
      961 tcgatgcact tagttcacta tatttcgcat attcaggagt accgggctga ccaagcattt
     1021 tcattgttaa gtaatacacg ctagaccgaa gatcccatgt gcaaatcata ttcagcttaa
     1081 gcatctctac aagttgccta aaatcatcgt caacaaactc cccacctcta atttcggaag
     1141 taatttgccg agattcaact tgtgctaact gatcaagttc actgagttga aaatcacttt
     1201 cagatacaaa tttaataaag tcaaatttag caatgcgttt atctctatat tcttttttat
     1261 aattatctac acagatcagg gtatcgtaaa aaagtatcgc ttgaataaga ttttcaaaag
     1321 cgactaaatc tccgttaata gtatctggat tcttaacaag aatatcaccc agtgttcttt
     1381 cgactgctgt taacgaagca ttatcaatca acgcataaga cattaaacta cctccattag
     1441 ataatatcta ataacattat atttcaatta ggtttaaaaa ttgtgatact tatgacagat
     1501 ttgagtgaca ttttatcaat ctactgattt tgatggtgaa atttattaga aggtatgcgt
     1561 agtgctacgg gagaaatgag aaacgcgccc agcaatggcg catcctttct taagcaagtt
     1621 ggctagtatc tatagcatcc atccaatcag gagcaattcc caaacgttcc gcgaactctt
     1681 cattggtcac gtcttccatt gagagactag ccagacaatc gttaatttca gaatagacgg
     1741 gggaaagtaa taggtattcc cgcaactctt tcaggtcttt ttccatctta acactccttg
     1801 ttaatagacc gtcgaacaat ggtacagatt tgtaccattc caagtactgg caagcaaccc
     1861 caccagcaca acaaattatc tcatacggta aaaacggtgt cgggctgttt ctttcggcta
     1921 gctcaatata tcaccttaaa caccagccgt tatcattgtc tcaattgggt ataccgtaat
     1981 gacaaggtga tacaaagtct caatagggtt gtataggaac attttgacta cacaaactgc
     2041 attatgtatc atttgcactt atattcacaa cccttttgag acaatgcaat gaacgttaga
     2101 atttactgcc gagcatcaac tgaggggcaa cacgctgatc gagcacttac cagcctacgt
     2161 gaattttcaa aatcaaaagg ctggcagatt gcaggagagt atatagaaaa tgctagcggg
     2221 gcgaagcttg agcgcgtaga gcttatgcgg ctattgtctg aagcccagtc cggcgatctg
     2281 ttgctagtgg aagctataga tcgtctgagt cgcctagaac atagtgcatg ggtcgaactg
     2341 aaagatacgc tcaaccgaaa agggttaatc atcgtatcaa tggatttacc aacgagctgg
     2401 cagatggtcg agatggcagg caatgatcta accagcggca tcctgagagc cgtaaatgcc
     2461 atgttgattg atatccttgc cacaatggca cggcaggatt acgaaacacg ccgtaaacgc
     2521 caacagcaag gcattgaacg agctaaaagc gaaggcatat acattgggag ggcgaaaaat
     2581 caggaagccc gagagatcgt tagagagatg ttggagcaag gagtaaaacc tgaactcatc
     2641 atgaaggcag caggaatcag ccgagcgact tactaccgga taaaaaacga gctattgatc
     2701 gtaaaatcag aataaaattt gaaaggtcgt caggcttgtt tcatcaggta aaaaccagca
     2761 tttctgatca caacgcttgg gggcacctat tggggcactc accaaaatcg aaagagaaat
     2821 aaactacaat tcaaattgtt agctaatttt tcgattcctg cagggacacc agataccctt
     2881 caaaccaaat ctaccttcat cccgtaaacg atgggtttgg cagcacactt gccctatatc
     2941 tactcatttt tactgcaaca ggttgaaatc tcagcactgt cagaaagcgc tgatgactaa
     3001 acagcccagg gccgggcgat gtaaccatca cacagaatcc tgatagcgaa atacggcgtg
     3061 actcgatact tcactccgca atgcattcct tgatgaattc gcaggcccgt gatacacggg
     3121 acaggtcgca gaatgacgac aatgtcctgg aaatcagcga accgtgtatc cggagtacat
     3181 ttgagcgact gtaccagaac atgaatgagg tgtttggatt aggcgattat tagcagggct
     3241 aagcatttta gtattattat tttccggttg aaggatatgg agatatcgac aacaaccgga
     3301 aaaagtttac gtctatattg ctgaaggtac aggcgtttcc ataactattt gctcgcgttt
     3361 tttactcagg aagaaaatgc caaatagcaa catcaggcag acaatacccg aaattgcgaa
     3421 gaaaactgtc tggtagcctg cgtggtcaaa gagtatccca gtcggcgttg aaagcagcac
     3481 aatcccaagc gaactggcaa tttgaaaacc aatcagaaag atcgtcgacg acaggcgctt
     3541 atcaaagttt gccacgctgt atttgaagac ggatatgaca caaagtggaa cctcaatggc
     3601 atgtaacagc ttcactaatg aaataatcca ggggttaacg aacagcgcgc aggaaaggat
     3661 acgcaacgcc ataatcacaa caccgataag taatgcattt tttggcccta cccgattcac
     3721 aaagaacgga ataatcgcca tgcacagcgc ttcgagtacc acctggaatg agttgagata
     3781 accatacagg cgcgttccta catcgtgtga ttcgaataaa cctgcataaa agacaggaaa
     3841 aagttgttga tcaaaaatgt tatagaaaga ccacgtccca acaataaata tgacgaaaac
     3901 ccagaagttt cgatccttga aaactgcgat aaaatcctct ttttttaccc ctcccgcatc
     3961 cgccgctacg cactggtgat ccttatcttt aaaacacatg ttgatcatca taaatacagc
     4021 gccaaatagc gagaccaacc agaagttgat atggggactg atactaaaaa atatgccggc
     4081 aaagaacgcg ccaatagcat agccaaaaga tccccaggcg cgcgctgttc catattcgaa
     4141 atgaaaattt cgcgccattt tttcggtgaa gctgtcaagc aaaccgcatc ccgccagata
     4201 ccccaggcca aaaaagagcg cccccagaat tagacctaca gaaaaattgc tttgcagtaa
     4261 cggttcataa acgtaaatca taaacggtcc ggtcaagacc aggatgaaac tcatacacca
     4321 gatgagcggt ttcttcagac cgagtttatc ctgaacgatg ccgtagaaca tcataaatag
     4381 aatgctggta aactggttga ccgaataaag tgtacctaat tccgtccctg tcaaccctag
     4441 atgtcctttc agccaaatag cgtataacga ccaccacagc gaccaggaaa taaaaaagag
     4501 aaatgagtaa ctggatgcaa aacgatagta cgcatttctg aatggaatat tcagtgccat
     4561 aattacctgc ctgtcgttaa aaaattcacg tcctatttag agataagagc gacttcgccg
     4621 tttacttctc actattccag ttcttgtcga catggcagcg ctgtcattgc ccctttcgcc
     4681 gttactgcaa gcgctccgca acgttgagcg agatcgataa ttcgtcgcat ttctctctca
     4741 tctgtagata atcccgtaga ggacagacct gtgagtaacc cggcaacgaa cgcatctccc
     4801 gcccccgtgc tatcgacaca atccacagac attccagcaa aatggtgaac ttgtcctcga
     4861 taacagacca ccaccccttc tgcaccttta gtcaccaaca gcatggcgat ctcatactct
     4921 tttgccaggg cgcatatatc ccgatcgttc tgtgtttttc cactgataag tcgccattct
     4981 tcttccgaga gcttgacgac atccgccagt tgtagcgcct gccgcaaaca caagcggagc
     5041 aaatgctcgt cttgccatag atcttcacga atattgggat cgaagctgac aaaacctccg
     5101 gcatgccgga tctccgtcat cgcagtaaat gcgctggtac gcgaaggctc ggcagacaac
     5161 gcaattgaac agagatgtaa ccattcgcca tgtcgccagc agggcaagtc tgtcgtctct
     5221 aaaaaaagat cggcactggg gcggaccata aacgtaaatg aacgttcccc ttgatcgttc
     5281 agatcgacaa gcaccgtgga tgtccggtgc cattcatctt gcttcagata cgtgatatcg
     5341 actccctcag ttagcagcgt tctttgcatt aatgcaccaa aaggatcatc ccccacccga
     5401 cctataaacc cacttgttcc gcctaatctg gcgattccca ccgcaacgtt agctggcgcg
     5461 ccgccaggac aaggcagtag ccgcccgtct gattctggca agagatctac gaccgcatcc
     5521 cctaaaaccc atactttggc tgacattttt ttcccttaaa ttcatctgac ttacgcatag
     5581 tgataaacct ctttttcgca aaatcgtcat ggatttacta aaacatgcat attcgatcac
     5641 aaaatgtcat agttaacgtt aacatttgtg atattcatcg catttatgaa agtaagggac
     5701 tttattttta taaaagttaa cgttaacaat tcaccaaatt tgcttaacca ggatgataaa
     5761 aatgacgcaa tctcgattgc atgcggcgca aaacgcacta gcaaaacttc acgagcgccg
     5821 aggtaacact ttctatcccc attttcacct cgcgcctcct gccgggtgga tgaacgatcc
     5881 aaacggcctg atctggttta acgatcgtta tcacgcgttt tatcaacatc acccaatgag
     5941 tgaacactgg gggccaatgc actggggaca tgccaccagc gacgatatga tccactggca
     6001 gcatgagcct attgcgctag cgccaggaga cgagaatgac aaagatgggt gtttttcagg
     6061 tagtgctgtc gatgacaatg gtgtcctctc acttatctac accggacacg tctggctcga
     6121 tggtgcaggt aatgacgatg caattcgcga agtacaatgt ctggctacca gtcgggatgg
     6181 tattcatttc gagaaacagg gtgtgatcct cactccacca gaaggaatca tgcacttccg
     6241 cgatcctaaa gtgtggcgtg aagccgacac atggtggatg gtagtcgggg cgaaagaccc
     6301 aggcaacacg gggcagatcc tgctttatcg cggcagttca ttacgtgaat ggactttcga
     6361 tcgcgtactg gcccacgctg atgcgggtga aagctatatg tgggaatgtc cggacttttt
     6421 cagccttggc gatcagcatt atctgatgtt ttccccgcag ggaatgaatg ccgagggata
     6481 cagttaccga aatcgctttc aaagtggcgt aatacccgga atgtggtcgc caggacgact
     6541 ttttgcacaa tccgggcatt ttactgaact tgataacggg catgactttt atgcaccaca
     6601 aagctttgta gcgaaggatg gtcggcgtat tgttatcggc tggatggata tgtgggaatc
     6661 gccaatgccc tcaaaacgtg aaggctgggc aggctgcatg acgctggcgc gcgagctatc
     6721 agagagcaat ggcaaacttc tacaacgccc ggtacacgaa gctgagtcgt tacgccagca
     6781 gcatcaatct atctctcccc gcacaatcag caataaatat gttttgcagg aaaacgcgca
     6841 agcagttgag attcagttgc agtgggcgct gaagaacagt gatgccgaac attacggatt
     6901 acagctcggc actggaatgc ggctgtatat tgataaccaa tctgagcgac ttgttttgtg
     6961 gcggtattac ccacacgaga atttagacgg ctaccgtagt attcccctcc cgcagcgtga
     7021 cacgctcgcc ctaaggatat ttatcgatac atcatccgtg gaagtattta ttaacgacgg
     7081 ggaagcggtg atgagtagtc gaatctatcc gcagccagaa gaacgggaac tgtcgcttta
     7141 tgcctcccac ggagtggctg tggtgcaaca tggagcactc tggcaactgg gttaacataa
     7201 tatcaggtgg aacaacggat caacagcggg caagggatcc gcgtcacact tcccccttca
     7261 cgaccttcaa taatatgcaa tgcagcttcc cgcccgataa tgtcatgtgg aagctgaatt
     7321 gtggtcagcg gcggtaaaaa cagatgcccg acgccaacca gattatcaaa gcccattacg
     7381 gcgacatcct gcgggatacg taccccctta gccaggagaa cctgataagc cacaaaggct
     7441 gcgcgatcgt taccacatat cagaacatca aaatctggtt tgcccgattt gaagtggtca
     7501 ttgagtaaac ttgcgagatc ggtgtagtga tcatcacctg ttgccatgtg aaattgtttc
     7561 acctcagcca gatctcgtcc agcatcacgc caggcctgct caaatccctg ccgacgatac
     7621 cctgttgcca acgcactttc cggtagccag aagcataacg gttgacgata gcccgccgcg
     7681 agcaaatgct gtgttgattc atattgtgca gtgtaatcat cagggatata actgggtaac
     7741 gctgggtcat ccgccacaca gttcgccaat acaatatttt caccatacag agactcaggt
     7801 agcgtgatat gtcgcagccc cattgtagta tagataatgc catccggacg gtgggcaagc
     7861 agctgacgtg ccgcgcgggc agcgtcatct tcagaaaaaa tattgattaa aaaactattc
     7921 cagccgaact cgctggcggt ttgctcaatg gcaagcagaa tatcaacaga gaaaggagtg
     7981 gtagcagtgt cctgcgccag cacggcgaga gtcgacggct tacgtccttg agcgcgcatc
     8041 ttacgggcgg aaagatcagg aacataattc agggtctgga ttgcctgcaa tacgcggtca
     8101 cgcgttgcag gacgcacaga ttctgcatta tgcatcaccc gggagactgt catcatcgac
     8161 actcccgcca ggcgtgcgac atcctttaat gaagccatac ccaagccgtt tgccgtaaaa
     8221 cgggcactgt agcagaaaca gacgtcactg gcgagatcca acgaaattct cgacgcccta
     8281 tcacccgaca cggcaataca ataaaaaata acaataattg ccggataatt gtacccaatt
     8341 ccgcctctgt tctcgccaac gagtgaccat ataggcaagt aagaaaaaca tgccaactgg
     8401 catcgccaac ctggagaaga aaatataaat atggatcatt gtgctgccgc tgtatcccga
     8461 catcagcccg gaaattattg cgattgctat cggttcaggc gcgattggct gcacgatcgt
     8521 tacggactcg cttttctggc tggtgaagca atattgcggc gctacgctca atgaaacatt
     8581 taaatactat acgacagcga catttatcgc ttcagtcgtc gctctggcgg gcacattcct
     8641 gctgtcattt atcatctaag cgcaaagaga cgtactatgg aaaacgctaa aatgaactcg
     8701 ctcatcgccc agtatccgtt ggtaaaggat ctggttgctc ttaaagaaac cacctggttt
     8761 aatcctggca cgacctcatt ggctgaaggt ttaccttatg ttggcctgac cgaacaggat
     8821 gttcaggacg cccatgcgcg cttatcccgt tttgcaccct atctggcaaa agcatttcct
     8881 gaaactgctg ccactggggg gattattgaa tcagaactgg ttgccattcc ggctatgcaa
     8941 aaacggctgg aaaaggaata tcaacaaccg atcagcgggc aactgttact gaaaaaagat
     9001 agccatttgc ccatttccgg ctccataaaa gcacgcggcg ggatttatga agtcctggca
     9061 catgcagaaa aactggctct ggaagcgggg ttgctgacgc ttgaagatga ctacagcaaa
     9121 ctgctttctc cggagtttaa acagttcttt agccagtaca gcattgctgt gggctcaacc
     9181 ggaaatctgg ggttatcaat cggcattatg agcgcccgca ttggctttaa ggtgacagtg
     9241 catatgtctg ctgatgcccg ggcatggaaa aaagcgaaac tgcgcagcca tggcgttacg
     9301 gtcgtggaat atgagcaaga ttatggtgtt gccgtcgagg aaggacgtaa agcagcgcag
     9361 tctgacccga actgtttctt tattgatgac gagaattccc gcacgttgtt ccttgggtat
     9421 tccgtcgcag gccagcgtct taaagcgcaa tttgccgagc aaggtcgtat tgtcgatgct
     9481 gataaccctc tgtttgtcta tctgccgtgt ggtgttggcg gtggtcctgg tggcgtcgca
     9541 ttcggactta agctggcgtt tggcgatcat gttcactgct tttttgccga accaacgcac
     9601 tccccttgta tgttgttagg cgtccataca ggattacacg atcagatttc tgttcaggat
     9661 attggtatcg acaaccttac cgcagcggat ggccttgcag ttggtcgcgc atcaggcttt
     9721 gtcgggcggg caatggagcg tctgctggat ggcttctata cccttagcga tcaaaccatg
     9781 tatgacatgc ttggctggct ggcgcaggaa gaaggtattc gtcttgaacc ttcggcactg
     9841 gcgggtatgg ccggacctca gcgcgtgtgt gcatcagtaa gttaccaaca gatgcacggt
     9901 ttcagcgcag aacaactgcg taatgccact catctggtgt gggcgacggg aggtggaatg
     9961 gtgccggaag aagagatgga gcaatatctg gcaaaaggcc gttaataacg tttcaacgca
    10021 gcatcgcaat cctttccctg ggtgagcgat gctgccgatg gcgcagactt aagatcccca
    10081 gtcttacccg ctataacccc ctgcttttac catcacccaa cgcctttcgc tgtaaacggc
    10141 ggtttcgcaa accaaaccaa aaccgtaagt aagataaaag caatagccgc catacggaaa
    10201 atctcatttg cagaaataga aagtgactgc tgggttattt cattattaat ttcattaaga
    10261 actcctgaaa gcgaaccata gtatttatcc ataatttgtg acgatgaatt aaacacgggg
    10321 ttaaattgat cgatggtttc tgtcaactga ctatggtgta acgattcgcg tcgtccccac
    10381 agcgtcattg tcaacgacgt accaactgat cctgacaagg tacgaaaaaa attactcata
    10441 ctcgaggcat tggcaaattt attatctggc aagcctgaaa acgaaatcgt tgttaagggt
    10501 aaaaagaaac aggcaacggc gaatccctga aaaaactgcg gcaaaatgat acctgtaaaa
    10561 tcaatcgttg gcataaatgt cacagaacgc cagtaatagc aaaccgcata catcaaaaaa
    10621 ctgaatgtca ccaacacccg catgtctatt ttgttgccat aacgtcctat caaaggtgaa
    10681 attaatagtg gcatgatgcc gatgggcgca taagcaagtc cggcccatat cgcattatac
    10741 cccatcgttt cctggagtaa ctgcggcata aggacgatcg ctccagagta aaataaatac
    10801 gcgcatgtga tactcacaat accaatggtg aagttacggg acttaaacaa actgagatca
    10861 agaatcgggt tctctgaggt cgactcccaa atgactaaag agatcagaaa aataactgat
    10921 actactgtta atattattat tgtactcgag ttgaaccaat ccagatcgcg ccctttatca
    10981 agcataattt gcaagccacc aacaccgagc actaacaggg tcagtcctgg tagattcatt
    11041 ttgaccggtg aagtctcagt ttctcttcct ttaagtaagg ttaagcataa tgtcaggacg
    11101 ataatcccca tagggacatt gattaaaaat atccaacccc agctaaagtt atcacaaata
    11161 taaccgccca atatcggccc acatatcgga gcgataatca cggtcattga ccataatgcc
    11221 agagcaaatg ttcttttttc tggtggataa ttccttaata ataaactctg tgacagtgga
    11281 attaacggcc ctgccattaa cccctgaacg actctaaaaa atatcagcac atcaagattg
    11341 atcgataaac tacacattaa ggaagacagc gaaaaaaaag tgactgaaag taaaaataac
    11401 cttaattcgc ctattctttg tgccagcctg ccggtaacag ggatcgcaat ggcatttgct
    11461 acgccaaacg aggtgataac ccaggtgcct tcgtctgttg atgctcccag aaagccagat
    11521 attgtcggta ttgcgacgtt agaaatagtg gaatccaaca tttgcataaa cgtcgctaat
    11581 gacaatgcaa tagtgacgca ccataacgtc ccaccggtta atggtgccgg agttgattta
    11641 gtgattgcca ttaaagttgt ccattatgcg aaataatgtt gctaatttct ttttctatcg
    11701 gactggtatc gataactaaa gccttactgg tataagccgg catggacgtc acggttgaag
    11761 ccagctcagg catctcagca atgtcttcgt tcttcgtatc aatagttgca gtcatcgata
    11821 aaccaatgcg caaggggtgt tccatgagtt cttttggatc aagagaaact tcaaccggta
    11881 cacgctgaac gattttgatc cagttccctg tcgcattttg tgcaggtaat aaggagaacg
    11941 cattgccggt tcccatattg atccctgtca cccggccatg aaacacaaca ttctcaccat
    12001 aaagatcgct gataatattg accgattgac caatccgtac atccgtgagt tgtgtttctt
    12061 taaagttagc attaacccac atttgacgtg ccggtacgac agccattaac gattgtccgg
    12121 gactcactgt ttcgccgacc tgaacacttc tctgggcaat atagccggta accggactct
    12181 taatatccgt acgtttaagc gccaaccagg cttctttggt tgcatccgca gcttcaacga
    12241 cttgtggctg acggtttaat ggtgtgttca ttactaaagc tttattcgct ttataagcct
    12301 ggatagcggc attcaatgcc gctttgctac ttattaacgt atctttggta tgctccagcg
    12361 tttcttttga aataaccccc tgcttcgcta aaggcacacg acggttataa tcttctaaag
    12421 attgttgata ctgaatacgt gctgaagcga cttcggcact gtattgttta tcctgtaagt
    12481 acagtttatt cgtttgccga acaatatttg ccagattatt tttagcttta ttgagtgcga
    12541 tagtggcatc agttttatcc agtgaaacta aaatgtcacc ctgtcgaacg tagttcgtat
    12601 ctttatgatt aacgacagtg acactacctg agacttgtgc agaaattgga tctgcattcc
    12661 ccgtgacata ggcgtcatct gtactaatca tgtcttctaa ttccattgac caataggcat
    12721 aagcacctga aaacgcaata aataaaacta tcgccaataa agaaaagtat tttcttctgt
    12781 tagaatgttt tttatttgaa ttaatctgtt ccactattat ctc
//
LOCUS       DAZFAB010000120        12428 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZFAB010000120 DAZFAB010000000
VERSION     DAZFAB010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.120"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..1326
                     /gene="xanP"
                     /locus_tag="ACHJWA_003986"
     CDS             <1..1326
                     /gene="xanP"
                     /locus_tag="ACHJWA_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFO4310489.1"
                     /translation="IYRLEDRPPLPQTLFAACQHLLAMFVAVITPALLICQALGLPAQ
                     DTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQGTSFNFVAPLIMGGTALKTGGAD
                     VPTMMAALFGTLMLASCTEMVISRVLHLARRIITPLVSGVVVMIIGLSLIQVGLTSIG
                     GGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNPYLRVASLVIAMAAGYALAWFMG
                     MLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLMLVFMITSLETIGDITATSDVSEQ
                     PVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCFGQNNGVIQLTGVASRYVGFVVA
                     LMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIAASGVRIVSREPLNRRAILIIAL
                     SLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGITAIVLNLIFPPEKQ"
     gene            1447..3156
                     /gene="yicH"
                     /locus_tag="ACHJWA_003987"
     CDS             1447..3156
                     /gene="yicH"
                     /locus_tag="ACHJWA_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO4310490.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTTLDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSSEVTSTE
                     PVQ"
     gene            complement(3209..5527)
                     /gene="yicI"
                     /locus_tag="ACHJWA_003988"
     CDS             complement(3209..5527)
                     /gene="yicI"
                     /locus_tag="ACHJWA_003988"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFO4310491.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAGRNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTALAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFSEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPVYVRDNTLLALGNNEQRPDYAW
                     HEGTAFQLFNLQDGHEAICEVPAADGSVLFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     IVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(5537..6919)
                     /gene="yicJ"
                     /locus_tag="ACHJWA_003989"
     CDS             complement(5537..6919)
                     /gene="yicJ"
                     /locus_tag="ACHJWA_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFO4310492.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDNFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLCGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            7212..7306
                     /locus_tag="ACHJWA_003990"
     tRNA            7212..7306
                     /locus_tag="ACHJWA_003990"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7246..7248,aa:Sec,seq:tca)
     gene            7502..8683
                     /locus_tag="ACHJWA_003991"
     CDS             7502..8683
                     /locus_tag="ACHJWA_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4310493.1"
                     /translation="MALTDVKVKTAKPKERPYKLADGGGMYLLINANGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKILAAGGDPGEAKKEEKIALQMSLKNTFEA
                     VAREWHQTKADRWSLRYRDEIIDTFEKDIFPYIGKLPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLTD
                     LAGYTGSIITKTATQIIMLTGVRTQELRFAHWEDIDFEAKLWEIPAEVMKMKRPHIVP
                     PSEQVIALFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHAQYLDGRREMMQWYADYIDSLSEL
                     A"
     gene            8782..9998
                     /locus_tag="ACHJWA_003992"
     CDS             join(8782..9087,9090..9998)
                     /locus_tag="ACHJWA_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611429.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEc31 family transposase"
                     /protein_id="HFO4310494.1"
                     /translation="MISSPQHKTGDLMNKKTKRTFTPEFRLECAQLIVDKGYSYRQAS
                     EAMNVGSTTLESWVRQLRRERQGIAPSATPITPDQQRIRELEKQVRRLEEHNTILKKA
                     NRALDVRLAERFTIVARLSDSHSVVSLCSALEIHRSSYQYWRKRRDTVNPARVRLCSE
                     IRRAWNQSRGSAGARTLAEMLTQNGVPMSRYRAGRLMKYLNLSSCQPGKHQYKNARQE
                     HTCLPNLLERQFAVPEPDRVWCGDITYIWAGNRWCYLAVVMDLFARRVIGWSLSANAD
                     TALISSALRMAYEVRGQPRDVMFHSDQGSQYTGLKYQQLLWRYRIKQSVSRRGNCWDN
                     SPMERFFRSLKTEWVPTDGYTGKDVARQQISSYILNYYNSVRPHHYNGGLTPEESENR
                     YHFYCKTVASIT"
     gene            10007..10159
                     /locus_tag="ACHJWA_003993"
                     /pseudo
     CDS             10007..10159
                     /locus_tag="ACHJWA_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0051495 - positive regulation of
                     cytoskeleton organization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
     gene            10249..10623
                     /locus_tag="ACHJWA_003994"
     CDS             10249..10623
                     /locus_tag="ACHJWA_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO4310495.1"
                     /translation="MKTLPDTHVREASRCPSPITIWQTLLGRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYVLVRTDQPGFSACTRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKHPEAK"
     gene            10620..11111
                     /locus_tag="ACHJWA_003995"
     CDS             10620..11111
                     /locus_tag="ACHJWA_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312567.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO4310496.1"
                     /translation="MMKLALTLEADSVNVQALNMGRIVVDIDGVNLAELINMVCDNGY
                     SLRVVDESDRTSADCTPPFTALTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGNGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAGCLPGLPT
                     FNW"
     gene            11123..11320
                     /locus_tag="ACHJWA_003996"
     CDS             11123..11320
                     /locus_tag="ACHJWA_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO4310497.1"
                     /translation="MTSLTPEAALDILIAWLQDNIDSESGIIFDNDEDKTDSAALLPC
                     IEQVREDVRTLRQLQLLQQNR"
     gene            11405..11746
                     /locus_tag="ACHJWA_003997"
     CDS             11405..11746
                     /locus_tag="ACHJWA_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310498.1"
                     /translation="MSGIIISRPEVDTGHTDVICSTSIRHVVTVRNAALQQTETLIRQ
                     LAEISVLTAADIGGKTALDRAMKQDFHCGCWLMEKPETAMKAITRNLDCEIWRDLMQR
                     SGMLSLMDAQG"
     gene            11807..11950
                     /locus_tag="ACHJWA_003998"
     CDS             11807..11950
                     /locus_tag="ACHJWA_003998"
                     /inference="COORDINATES: protein motif:HMM:NF025085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310499.1"
                     /translation="MNAFKQLHQSKDEVFERGVINVFRGPNGITKPIPPVNSAVKLSS
                     TTW"
     gene            11944..12363
                     /locus_tag="ACHJWA_003999"
                     /pseudo
     CDS             11944..12363
                     /locus_tag="ACHJWA_003999"
                     /inference="COORDINATES: protein motif:HMM:NF025085.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
BASE COUNT         3151 a         3226 c         3132 g         2919 t
ORIGIN      
        1 atttaccgtc ttgaagatcg tccgccgctt cctcaaaccc tgtttgccgc ctgtcagcat
       61 ctgctggcga tgttcgttgc ggtgatcacg ccagcgctat taatctgcca ggcgctgggt
      121 ttaccggcac aagacacgca acacattatt agtatgtcgc tgtttgcctc cggtgtggca
      181 tcgattattc aaattaaggc ctggggtccg gttggctccg ggctgttgtc tattcagggc
      241 accagcttca actttgttgc cccgctgatt atgggcggta ccgcgctgaa aaccggtggt
      301 gctgatgttc ctaccatgat ggcggctttg ttcggcacgt tgatgctggc aagttgcacc
      361 gagatggtga tctcccgcgt tctgcatctg gcgcgccgca ttattacgcc gctggtttct
      421 ggcgttgtgg tgatgattat cggcctgtcg ctaattcagg ttgggttaac gtccattggc
      481 ggcggttacg cagccatgag cgataacacc ttcggcgcac cgaaaaatct gctgctggca
      541 ggcgtggtct tagccttaat tatcctgctt aaccgtcaac gtaaccctta cttacgcgtg
      601 gcctcactgg tgattgcgat ggcggccgga tatgcgctgg cgtggtttat gggcatgttg
      661 ccagaaagca acgaaccgat gacgcaagaa ctgattatgg tgccaacgcc gctctattac
      721 ggtcttggca ttgaatggag tctgctgctg ccgctgatgc tggtctttat gatcacttcg
      781 ctggaaacca ttggcgatat cacggcgacc tctgacgttt ccgaacagcc agtgtccggt
      841 ccgctgtaca tgaaacgcct gaaaggcggc gtgctggcaa acggcctgaa ctcgtttgtt
      901 tcggcggtgt ttaacacctt cccgaactcc tgcttcgggc aaaacaacgg agtgatccag
      961 ttgactggtg ttgccagccg ctatgtcggt tttgtcgtcg cgctgatgtt gatcgtgctg
     1021 ggtctgttcc cggcagtgag cggttttgta caacacattc cagaaccggt tctgggcggc
     1081 gcaacgcttg taatgtttgg caccatcgcc gcctccggtg tgcgtatcgt ttctcgtgag
     1141 ccgctgaacc gtcgggcgat tctgattatc gcgctgtcgc tggcggttgg tctgggcgtg
     1201 tctcagcagc cgctgatttt gcagtttgcc cctgaatggc tgaaaaacct gctctcctcc
     1261 gggatcgccg cgggcggtat tactgccatc gtgctgaatc tgattttccc accagaaaaa
     1321 cagtaattca ctggcggcgg taagataatt accgccgcag tctcacctct ttcaccattc
     1381 ccgccttgag gattgcgcgt aaatcgtgca taactccctt atgtgcattt cacgggatgg
     1441 aagaccatga aatttattgg gaagctgctt ctctacattc tcatcgctct gttagtggtg
     1501 atcgctggcc tctattttct tctgcaaacc cgctggggag cagaacatat cagcgcatgg
     1561 gtttccgaga atagcgacta tcatctggcc ttcggggcga tggatcaccg tttttccgcg
     1621 ccatctcata tcgtgctgga gaacgtcacg tttggtcgtg atggtcagcc cgcgaccctg
     1681 gtggcaaaaa gtgtcgacat tgcgctaagc agtcggcaac tgaccgaacc acgccatgtc
     1741 gataccatcc tgctggaaaa cgggacgctg aatctcaccg accagaccgc gccgctaccg
     1801 ttcaaagccg atcgtctgca actgcgtgat atggcgttta atagcccgaa tagcgaatgg
     1861 aaactgagcg cgcagcgggt aaatggcggc gtggttccgt ggtcaccaga agccggtaaa
     1921 gtgctgggta cgaaggcgca gattcagttt agtgccggat cgctttcgct caatgatgtt
     1981 cctgccacca atgtactgat tgaaggcagt attgataacg atcgcgttac gctgactaac
     2041 ctgggtgccg acatcgcccg cgggacatta accggaaacg cgcagcgtaa cgccgacggc
     2101 agctggcaag tggaaaatct gcgcatggcg gatatccgtc tacaaagcga aaaatcgcta
     2161 accgacttct ttgcgccatt acgctctgtc ccgtcgttgc agattggtcg cctggaagtg
     2221 atcgatgctc gtttgcaagg tccggactgg gcggtgaccg acctcgatct cagcttgcgc
     2281 aacatgacct tcagtaaaga tgactggcag acacaagaag gcaaactgtc gatgaacgct
     2341 agcgagttca tttatggttc gctgcattta tttgacccga ttataaacgc ggaattttcc
     2401 ccgcagggcg tagcgctgcg ccagttcacc agccgctggg aagggggtat ggtcagaacg
     2461 tcagggaact ggctgcgtga cgggaaaacg ttgatccttg atgatgcggc aattgccggg
     2521 ctggaatata ccttgccgaa aaactggcaa cagttgtgga tggaaacgac acccggttgg
     2581 ttaaacagcc tgcaactgaa gagatttagc gccagccgca atctgatcat tgatatcgac
     2641 cctgacttcc cgtggcagct caccacgctc gatggttacg gtgccaacct gacgctggtt
     2701 accgatcata aatggggcgt ctggagtggc tcggcgaatc tgaatgccgc cgccgcgaca
     2761 ttcaatcgtg ttgatgttcg tcgcccgtcg ctggcgctga ccgccaacag cagcacggtg
     2821 aatatcagcg aactgagtgc atttactgaa aaaggcattc tggaagccac tgccagtgtt
     2881 tcacaaacgc cacaacgtca gacccatatc agcctgaatg gacgcggtgt gccggtgaat
     2941 attttgcaac aatggggatg gcctgaatta ccgttgactg gcgacggcaa tattcagctt
     3001 accgccagtg gcgatattca ggccaatgtc ccgctgaaac ctacggttag cgggcaactc
     3061 catgccgtga acgccgcaaa gcagcaagtg actcaaacca tgaatgcggg cgtcgtttcc
     3121 agtagcgaag ttacatcgac agagccggtg cagtaaaaat caatctcgcc gggtacgact
     3181 cagacgtacc cggcattcca tcaatagatt acaacgtaat tgtcagcgca ttcccttgag
     3241 gcgtcaccac cagcccctgc tcactttcag cctgcgaacc accttgcaga ccatttactt
     3301 tcacaatatt gcgcaagcac agtgtccagt tcttcgcctc gcccgcacca gtcacagtaa
     3361 tcgtgttgcc agtacgcgcc gctttcaggg tgaaaagaac ggaaccgtca gcagcgggca
     3421 cttcacagat ggcttcatgc ccgtcttgta gattaaagag ctggaatgcc gtgccttcgt
     3481 gccacgcgta atcgggacgt tgctcgttgt tgcccagcgc cagtagggtg ttatcacgca
     3541 cataaacggg cagactcagg aagctgtgct gctgtttatg ccagcgacta ccatcgagtt
     3601 catcgttgtg ccacagatgt gtccagcgac cttccggcaa gtagaactgc acatcgcccg
     3661 cttcactgaa caccggagca accatcacgt tgtcgcctaa catgtattga cggtcaaggt
     3721 aatcacaagc cggatcgtcc gggaactcca tcatcatggc ccgcatcatc ggcgtacccc
     3781 gcgcgttcgc acgagcagct tcacgataca gatacggcat catgcggcat ttcagttgcg
     3841 tgaagaagcg caccacatca caggactcat catcgtaggc ccacggcaca cgataagatt
     3901 tgctgccatg taaacggcta tggctggaga gcaaaccaaa cgcgcaccag cgtttgtaaa
     3961 cgtgcgccag agcggtattc tcaaagccgc cgatatcgtg gctccagaaa ccaaaacctg
     4021 aaaggccaat agacaaacca ccgcgcaggc tttccgccat tgattcgtag ttagcgtaac
     4081 agtcaccacc ccagtgtacc gggaatttct gcgcaccaac ggaggccgag cgagcaaaca
     4141 agacggcttc ttcctcgcca acggtgtcct tgagcacgtt ccacaccagt tcgttgtaga
     4201 tgtacgcata atggttatgc attttctgcg gatcggaacc gtcaaaccac tgaacatcga
     4261 ttggaatacg ttcgccaaag tcggtcttaa agcaatcaac gcccatcgcg accagacctt
     4321 tcagtttgtc ggcgtaccat ttgcaggcat ccggattggt aaagtcataa atcgccagac
     4381 ctggctgcca tttatcccac tgccataacg aaccgtccgg gcgtttgagt aaatagcctt
     4441 tctcttgtaa ctctttaaag acaggggatt tttgcccgat ataggggtta atccagacgc
     4501 agatttttag ccctttcgct ttcaggcggc ggatcatccc ttccgggtcc gggaaagtca
     4561 gcgggtccca ctcaaaatcg caccactgga aggctttcat ccagaaacag tcaaagtgaa
     4621 aaacatgcag tggcagattg cgtcccgcca taccatcgat aaagctgttt accgtcgctt
     4681 cgtcgtagtt ggtggtaaat gaagtggtta gccacaggcc gaaggaccac gcgggcggca
     4741 gcgccggacg accagtaaaa cgggtataac gatcaagtac cgctttcggc gtcgggccgt
     4801 cgataacaaa gtattcgaga tattcactct caacgctgaa ctgcactttg gagactttct
     4861 ccgatcccac ttcaaaagag acgcattgag gatgattgac cagtacccca tagccacggt
     4921 tagtcatata gaacgggata tttttatacg cctgttcagt acttgtgccg ccgtcccggt
     4981 tccaggtctc taccgtctgg ccattgcgca ccagggcagt aaagcgctct cccagaccgt
     5041 aaactgtttc gccaacgcca agatccagcc gctcaaacat ataatttcgt tgattattcg
     5101 tgtcctgcac gtagccatta tttttcacct gactaccggt aatacgttcg ccgttgcgca
     5161 gaaaatccag tgaccagaac tcacctttgc tgacacgcgc gcttaagttg ccacttttaa
     5221 actcagcgta acgttctgtg ttttcgattg tgaccttcac gtcctgcaaa atattgagcg
     5281 gataatgagg accgttattc agcgccccct gaaaatgctc aatccgtaca ccgacaatac
     5341 cttcctgtgg ggagaaaaag cgcagcgtaa ataaaggcgt atcaagctgc caggttcgtt
     5401 cacgcacatc acggggggca gcatagacca ccatttcatt accctgctgt tcaacctcga
     5461 acacctgaag cgggtgaatc aaattgaggc caggttgaat caaccagttt ccgtcgctaa
     5521 ttttcattgc cgttccttag ttctgcactt cttgagaggt gaattgttgc tggcaacggc
     5581 gtttgccctg ggccagctgt tccataacgg ttttcagatt gtgcgtcgtg agtgagtagt
     5641 agcgtttagc gataatcgcg ctcagcaaat aacagatcgc cggaacaatc gtgaatagcg
     5701 caataatgat gctaatcgtg gcgctgttct gcgctttttc tgccgcatca tatccgccat
     5761 aagccagcat ccagccgata agagcgccgc cgaaggccaa ccccagtttg agcacaaaca
     5821 gcgtgccagc aaaactgatc ccggtcaggc gtttaccatt gcaccactcg ccgtagtcga
     5881 cggtatcgga catcattacc cactggatag gtgtcaccag ttgatgcaat acaccaatca
     5941 cgaagatgaa gacaaacata gtgatgctgg cctgcatagg aacaaagaac atcgcgaggc
     6001 taatcactgc cagcagggcg ttcgtccacc agaagatagt gactttacat ttccagtcgg
     6061 tcagcggttt tgccagtgcg gaaccaatca ggttacccac gcaataagtg gtgagaaaag
     6121 cgacaaacac ttccggcgta cccaaaatcc atgtgacgta atacatcatc gctccaccgc
     6181 ggacgcacac cgccaggata ttgaaaatgg ttagtaaacc gacaatccgc cactggtcgt
     6241 tttgccagat atcacgtaaa tcttcccgca tagacgtcgt tgtaggtggt gcttcaacgc
     6301 gttctttagt ggtgaagaaa cagaatgcca gcatcatgaa tgccaccacg gaaaggaccg
     6361 cgataccgcc ctggaaaccg agtggtttat tatcaccgcc aattaaatta accagtggca
     6421 tcatcagaac agtagagagc atgcctcccg cggtcgccag cacaaaacgc caggattgca
     6481 gcgagatacg ctgagtcggg tcattggtga ttacaccacc caatgcgcag taagggatat
     6541 tgacgacggt ataaagtaag gtaagtaggg tgtaagtaat tgctgcatag atcattttgc
     6601 cgttcatact gagatctggc gtgctatagg ccagtacaca gacgatccca aatggcagtg
     6661 cgccacacag tacccacgga cgaaatttac cccagcgaga gcgcgttcga tcggccaaca
     6721 accccatgca aggatcggaa atcgcatcca gtgcacgagc gaccaaaaac atggttccga
     6781 caaatccggc aggtatgcca aaattatcgg tataaaagaa catcatatat aacattacgt
     6841 tatcgaaaat aatgtggctg gcggcgtctc ccatgccata accaattttc tctttaacgg
     6901 acaacacttc actcttcatc tgcttttcct tgatgctata ggggtgctga gaccgataac
     6961 gcattttgta aaccatccct gtgagagcga atattccgat atctggttat taatttatga
     7021 ttcttgtttt atgtgatcgt ggtagcgtta attccgctca tatatcattg taaaatatga
     7081 gttttatatg aattataatg ctttcgtgat aatacgctgc gtgtattagg cggaaaaaac
     7141 tgatctgggg gatgtagaaa ctcaaggaag tagctataat gcgccccgcc tccatgtagc
     7201 aatcgaggcg cggaagatcg tcgtctccgg tgaggcggct ggacttcaaa tccagttggg
     7261 gccgccagcg gtcccgggca ggttcgactc ctgtgatctt ccgccagttt gcctctcctg
     7321 aactctcccg acatcaacaa agccccttat aatcccttat atatcaggtt ttctttgtat
     7381 cctgaggtca aacgacttta acccatatca agttgtttgt gggggtgcat tgggggtatc
     7441 aggttggtta ctggcggaaa ttcccccagg aggatggatt catcaaaagc tggaggatgt
     7501 catggcgcta accgatgtta aagtgaaaac cgcgaagcca aaagaaaggc cctataagct
     7561 ggctgatggc ggaggtatgt atctgctgat taacgctaat ggttccaaat actggcgtat
     7621 gaagtaccgc ttcgccggta aagaaaagat gctgtctatt ggcgtatacc ccgatgtaac
     7681 gcttgccgac gcacgtgaga aacgcagtga agccaggaaa atacttgcgg ctggtggcga
     7741 tccgggtgag gcaaaaaaag aagaaaaaat agcgctgcaa atgagtttga aaaacacttt
     7801 cgaggccgtt gctcgcgagt ggcatcagac gaaagccgat cgctggtcac tgcgctaccg
     7861 cgatgaaata atcgacacct ttgagaaaga tatcttcccc tacatcggca agctacctat
     7921 tgccgaaatc aagccgatgg agctattgga agcactacgt aagatggaga aacgtggagc
     7981 actggagaaa atgcgcaagg tccgccaacg ttgtggtgag gtgtttcgct atgcaattgt
     8041 tactggtcga gccgactata accctgctcc cgatcttgcc agtgctttag ctacaccgaa
     8101 aaaagtacat tttccctttc ttactgccaa tgaacttccc cacttcctta ctgatctggc
     8161 gggttatacc ggaagtatca tcactaagac agctactcag atcattatgt taaccggtgt
     8221 acgaacgcaa gagttgcgtt tcgcgcattg ggaggatatt gattttgagg caaaattatg
     8281 ggagatcccg gcagaagtaa tgaagatgaa acggcctcat attgtgccgc cctctgagca
     8341 ggttattgcg ctatttaagc aacttgaacc aatctcaaaa catcatcctc tggtctttat
     8401 cggtaggaac gatcctcgca agccaattag taaggagagc attaaccagg tcattgaatt
     8461 gctggggtat aagggaagac tcacagggca cggtttcagg cataccatga gcacaattct
     8521 gcacgaacaa ggctttaatt ctgcctggat tgaaatgcag ttggctcatg tggataaaaa
     8581 ctccatcagg ggtacctata accacgccca gtatctcgat ggtcgccgtg aaatgatgca
     8641 atggtacgct gactacattg attcactttc tgagctggcc tgatgaatga aagttcggtg
     8701 acacaaaaat aatgaataac catccagccc caacaggcgg atggttgtag tggcacagta
     8761 aatttggcca cctgattaaa ggtgatatcc tcaccacaac acaaaacagg tgacttaatg
     8821 aacaagaaaa ccaaacgtac tttcacccct gaattcaggc tggaatgtgc acagctaatt
     8881 gttgataagg gctactcata tcgacaagcc agtgaagcga tgaatgtcgg ttctaccacg
     8941 cttgagagtt gggtgcgcca gctcaggcga gagcgtcagg ggattgcgcc ctctgccaca
     9001 cctattactc cagaccagca acgtatccgc gaactggaaa agcaggttcg ccgcctggag
     9061 gaacacaata cgatattaaa aaaggctaca accgcgctct tgatgtccga ctcgctgaac
     9121 ggttcacgat agttgccaga ctgagtgaca gccactcagt tgtcagcctc tgttctgctc
     9181 tggaaataca ccgcagcagt taccagtact ggcgaaaacg acgcgatacg gttaatccgg
     9241 cgcgagtcag gttgtgcagc gaaatacgcc gggcgtggaa ccaaagtagg ggctctgcgg
     9301 gggcgcgcac gctggctgaa atgctgaccc aaaacggcgt cccgatgagc cgttaccgtg
     9361 ccgggcgtct gatgaaatat ctgaacctga gcagttgtca gcccggaaaa catcagtaca
     9421 aaaatgctcg tcaggaacat acctgcctgc cgaatctgct tgagcgccag ttcgctgtgc
     9481 ccgagccaga tagggtatgg tgcggagata ttacgtatat ctgggcagga aatcgctggt
     9541 gctatctggc ggtcgtcatg gatctttttg cccgcagggt tatcggctgg agcctgtcag
     9601 cgaatgccga tactgccctg ataagcagtg ccctgcggat ggcgtatgag gtgcgtggtc
     9661 agccgcggga cgtcatgttc catagcgacc agggaagtca atatacagga ctgaaatatc
     9721 aacaacttct ctggcgttac aggataaagc aaagtgtcag ccgacgggga aactgctggg
     9781 ataacagtcc aatggaacgc ttcttccgca gtctgaaaac agaatgggtg ccaaccgatg
     9841 gctacacagg caaagacgtg gcccggcagc aaatcagcag ttatattctg aattattaca
     9901 atagcgtcag gcctcaccat tataacggag ggctgacgcc ggaagagtca gagaacagat
     9961 atcattttta ctgtaaaacc gtggccagta ttacttgacc actacactgg aactcatgct
    10021 caccagcggt gagctgaatc cccgccatca gcataccgtc acgctgtatg caaaagggct
    10081 gacctgcaaa gccgataccc tcggcagtcg aggttacgtt tatatggctg tttatccgac
    10141 gcccgaaacg aaaaagtaaa tcatcagaat aaccttctgc tccggcctgg tgttttcacc
    10201 acgccacttc cccatttctc atctctgcat atcaggaaaa actttagtat gaaaacatta
    10261 cccgatacac acgtacggga ggcatcgcgc tgcccgtctc ccatcaccat ctggcagaca
    10321 ctgcttggcc gtctgctgga ccagcactac ggcctcacgc tgaatgacac accgttcgct
    10381 gatgaacgtg tgattgagca gcatattgag gcaggtattt cactgtgtga tgcggtgaac
    10441 tttctcgtgg aaaaatacgt gctggtgcgt accgaccagc cgggattcag cgcctgtacc
    10501 cgctctcagt taataaacag catcgatatc ctccgggctc gcagggcgac cggcctgatg
    10561 acccgcgaca attacagaac ggtaaataac attaccctgg gtaagcatcc ggaggcgaaa
    10621 tgatgaaact ggccctgaca ctggaagccg acagcgttaa cgtacaggca ctgaatatgg
    10681 ggcgaattgt cgttgacatt gatggtgtta atctcgctga actgattaac atggtctgcg
    10741 ataacggcta ctcccttcgt gttgttgatg aatctgaccg gacctcagca gactgcacgc
    10801 caccatttac tgcccttacc ggcatacgct gcagtaccgc acatatcacg gaaacggaca
    10861 acgcctggct gtactcgctg tcacaccaga ccagtgactt cggtgaatca gaatggattc
    10921 attttaccgg taacggctat ctgttgcgta ccgatgcgtg gtcgtacccg gttctgcggc
    10981 ttaaacgcct gggtctgtca aaaacgttcc gtcgtctggt cgtcacactc atccggcgtt
    11041 atggtgtcag tctcattcat ctggatgcca gtgccggatg tctgccaggt ttacccactt
    11101 ttaattggta actaggaaca acatgacatc attaaccccg gaagcagcac tggatattct
    11161 gattgcgtgg ctgcaggaca atatcgacag cgaatccgga attatcttcg acaacgatga
    11221 ggataaaacg gattcggcag cattgttgcc ctgtatcgaa caggtcaggg aagatgtccg
    11281 taccctgcgc caactgcagc ttctgcaaca gaaccggtga gtctcactca ccatacttca
    11341 ttccactgac gccagcctga acagggctgg cgttttcgtt tataaaccaa aaggaaaacc
    11401 gataatgtca ggcatcataa tctcccgtcc ggaagtggac accgggcata cggacgttat
    11461 ctgctccacc agtatccgac acgttgtgac ggtacggaat gcagcactgc aacaaaccga
    11521 aacgcttatc cggcaactgg cagaaatctc agtcctgaca gcagcagaca ttggcggcaa
    11581 aaccgccctg gaccgggcga tgaaacagga ttttcactgc ggctgctggc tgatggagaa
    11641 acctgaaacc gcaatgaaag ccatcacccg caacctcgac tgcgaaatct ggcgtgacct
    11701 gatgcaacgc tccgggatgc tgagtctgat ggatgcacag ggctaggata catggtaccg
    11761 ctcactggag tacgataata ttccggaaat cagcgaggcg aatatcatga atgcattcaa
    11821 acaactgcac cagagtaagg atgaagtgtt tgagcgtggg gtgatcaacg tcttcagagg
    11881 gccgaacgga attacaaaac caattccccc tgtaaattcg gcagtaaaat tatcgtcaac
    11941 aacttggtga gatgggacag atggagattt catcttatca gtggaatgca ggcagatcgc
    12001 ttagctgacc tggaaagaat gttgcatctg ctcagcggta aaccgctccc caacaaccga
    12061 gggaatatca ccattaatct ggatgaccac ctacagtccg ttcagggtaa agagcgctat
    12121 gaagacaagt aaatacttta agaagggccc gcacacatca cattcagaaa gccagagctg
    12181 gttgacaggc ttaatgatat tattgccaga cactacctag gaatgttacc ttcacaaaaa
    12241 tctctaagaa ttgattcaat tgtgtatttt agcctcattc cctcatgtaa agtcatttgg
    12301 gggttattat gggggtcaat catttatcca acacgcatta atcatttaaa atcaatgcat
    12361 taataatcat attcgactcc tgtgatctcc gccaaacttc ctcttctgaa ctctcccgac
    12421 gtcaacaa
//
LOCUS       DAZFAB010000121        12106 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZFAB010000121 DAZFAB010000000
VERSION     DAZFAB010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12106
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.121"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            79..681
                     /gene="yebB"
                     /locus_tag="ACHJWA_004000"
     CDS             79..681
                     /gene="yebB"
                     /locus_tag="ACHJWA_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFO4310500.1"
                     /translation="MNINYPAEYEIGDKVFTCIGDALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(683..1204)
                     /gene="ruvC"
                     /locus_tag="ACHJWA_004001"
     CDS             complement(683..1204)
                     /gene="ruvC"
                     /locus_tag="ACHJWA_004001"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFO4310501.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(1239..1979)
                     /gene="yebC"
                     /locus_tag="ACHJWA_004002"
     CDS             complement(1239..1979)
                     /gene="yebC"
                     /locus_tag="ACHJWA_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO4310502.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(2008..2460)
                     /gene="nudB"
                     /locus_tag="ACHJWA_004003"
     CDS             complement(2008..2460)
                     /gene="nudB"
                     /locus_tag="ACHJWA_004003"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFO4310503.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(2578..4350)
                     /gene="aspS"
                     /locus_tag="ACHJWA_004004"
     CDS             complement(2578..4350)
                     /gene="aspS"
                     /locus_tag="ACHJWA_004004"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFO4310504.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLRSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPAALAELSIQVVKKAENN"
     gene            4660..5226
                     /gene="yecD"
                     /locus_tag="ACHJWA_004005"
     CDS             4660..5226
                     /gene="yecD"
                     /locus_tag="ACHJWA_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO4310505.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILNAL"
     gene            5223..6041
                     /gene="yecE"
                     /locus_tag="ACHJWA_004006"
     CDS             5223..6041
                     /gene="yecE"
                     /locus_tag="ACHJWA_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFO4310506.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNQVIL
                     DSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            6094..6489
                     /gene="yecN"
                     /locus_tag="ACHJWA_004007"
     CDS             6094..6489
                     /gene="yecN"
                     /locus_tag="ACHJWA_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFO4310507.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            6530..7273
                     /gene="cmoA"
                     /locus_tag="ACHJWA_004008"
     CDS             6530..7273
                     /gene="cmoA"
                     /locus_tag="ACHJWA_004008"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFO4310508.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            7270..8241
                     /gene="cmoB"
                     /locus_tag="ACHJWA_004009"
     CDS             7270..8241
                     /gene="cmoB"
                     /locus_tag="ACHJWA_004009"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFO4310509.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(8406..10835)
                     /gene="torZ"
                     /locus_tag="ACHJWA_004010"
     CDS             complement(8406..10835)
                     /gene="torZ"
                     /locus_tag="ACHJWA_004010"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFO4310510.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGYNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFAELAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAQLRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDQPAVQA"
     gene            complement(10860..11960)
                     /gene="torY"
                     /locus_tag="ACHJWA_004011"
     CDS             complement(10860..11960)
                     /gene="torY"
                     /locus_tag="ACHJWA_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFO4310511.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGFLMAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFEAHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKLLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT         2780 a         3200 c         2941 g         3185 t
ORIGIN      
        1 atatacattg cgcctcctga aaaaataaat tttttatgct attacgtata ttcgtatcta
       61 tttcaatgga atgacaacgt gaatattaat taccctgctg aatatgaaat tggagataaa
      121 gtctttacat gtataggtga tgccttattt ggtcaaatat cagctgcatc aaattgctgg
      181 agtaatcacg tcgggatcat tatcggtcat aacggtgaag acttcctggt tgcagaaagc
      241 cgggttcccc tttcaaccat caccacgcta tctcgtttta ttaaacgttc gtctaatcaa
      301 cgctatgcta taaagcgatt agacgctggg ctgacagaac aacaaaaaca acgaattgtt
      361 gaacaggttc cctcccggct acgcaaactt taccacaccg gttttaaata cgaatcttcg
      421 cgccagttct gttcaaaatt tgtttttgat atttataaag aagcgctatg tattccggtg
      481 ggtgaaatag agacgtttgg agaattgtta aatagcaatc cgaatgcaaa actcactttc
      541 tggaaattct ggttcctggg ttctattccg tgggagcgta aaaccgtcac gccagccagt
      601 ttgtggcatc atccaggttt ggtgttgatt catgcggagg gagtggaaac gcctcagccg
      661 gaactgaccg aggcggtata acttaacgca gtcgccctct cgccaggttc agccgcgatt
      721 cgctcatctg catcgcattc tgactaacgt ggcagtgggt gatggcaatc gccagcgcat
      781 cggcggcatc cgcctgtgga ttagcgggca gtttcagcaa ggtgcggacc atatgctgca
      841 cctggctttt ttcggcacta ccaataccta ccaccgtttg ctttacctga cgtgccgcgt
      901 attcaaatac tggcaactcc tgattcaccg ccgccacaat cgccacgccg cgcgcctggc
      961 ccagtttcag ggctgagtca gcgttctttg ccataaagac ttgttcaatg gcgaaataat
     1021 cgggctggaa ctgggtgatg atttccgtca cgcccgcata gatgagtttc agacgagacg
     1081 gtaaatcatc cactttggtg cgtatgcatc cgctacccag gtaggacagt tgcctaccta
     1141 cctggcggat gacgccgtag ccggtcacgc gcgaacccgg atcaatgccg agaataatag
     1201 ccatcacgcg tctccgtttt gctgtttagc aggcctcatc agagagtcgc tgcgacctca
     1261 tcagagattt caccgttatg gtaaacttcc tgcacgtcgt cgcaatcttc cagcatatcg
     1321 atcagacgca tcagtttcgg tgcggtttct gcatccatat cagctttggt agacgggatc
     1381 atggaaactt ccgcgctgtc tgctttcaga cctgccgctt ccagagcgtc gcgcacttta
     1441 cccatttctt cccatgcagt gtagacatca atcgcgccgt catcataggt cacaacgtct
     1501 tcagcacctg cttccagtgc tgcttccatg atggtgtctt catcgccttt ctcgaaggag
     1561 atgacgcctt ttttgctgaa cagataggct acggaaccat cagtaccgag gttaccgcca
     1621 catttgctga atgcatgacg cacttcagca acggtacggt tgcggttgtc agacagacat
     1681 tcaatcatga ttgccgtgcc gccaggaccg taaccttcgt agatgatggt ttccatgttc
     1741 gcatcatcat caccgcccac accacgtgca attgcgcggt tcagagtgtc acgggtcatg
     1801 ttgttagaca gtgctttatc aattgctgca cgcagacgcg ggttagcgtc cggatcgcca
     1861 ccgcccagct tagccgcggt taccagctca cgaatgattt tagtgaagat tttaccgcgc
     1921 ttagcatcct gcgcagcttt acgatgtctg gtgttggccc atttactatg acctgccata
     1981 aaaatatctc cagatagccc tgcctgttca ggcagcgtta attacaaact gttcaatcgc
     2041 ctgccggttg ctccaggact tagtgagcgc cgccgcagca ggcgcatcaa gccacttgta
     2101 agccagatgt tcagtgaaaa cgatctgccg ctcatgcgga agcgcaagac agaaccatga
     2161 ttccgtatta cgcgtcacgc ccggcgcata gcgatgacgt aaatgtgaaa aaatttcaaa
     2221 ctctaccgtg cgctgacagt caattaaggt cagttgttca gcgacaacat caatggtgac
     2281 ctcttccttt acttcgcgca tggcagcttg cggcgcggtt tcaccctctt ccacgctgcc
     2341 ggttaccgac tgccagaaat cggggtcatc acgccgctgc aacatcagca cccgtttcgt
     2401 atcttgtgcg tagatgacca ctaagatcga aacgggacgc ttatacactt tatccttcac
     2461 gctgcctctt cgttgactgc cttcgctcat cccattcaca tagttatcta tgctcatggg
     2521 agttcactca gttgccgcct cgatgcaacg cgaatgattt cgtgtatttg agtcatatca
     2581 gttattctca gccttcttca caacctgaat gctcagctca gccagtgcag ccgggttagc
     2641 aaagctcggt gcttcagtca tcagacacgc tgccgccgtg gttttcggga aggcgataac
     2701 gtcacggata ttgtcggtgc cggtcagcag catggtcaga cggtcaagac cgaatgccag
     2761 acctgcgtgc ggcggagtac cgtatttcag agcgtcgagc aggaagccga atttctcgcg
     2821 ctgttcctct tcgttgatac ccagaatacc aaacaccgtc tgctgcatat caccattatg
     2881 gatacgtact gaaccaccgc ccacttcgta accattgatg accatatcgt aagcgttcgc
     2941 caccgcattt tccggtgcag ctttcagctc tgcagccgtc atgtctttcg gtgaggtgaa
     3001 cggatggtgc attgccgtca ggccgccttc accgtcgtct tcaaacatcg ggaagtcgat
     3061 aacccacagc ggtgcccatt tgctttcgtc ggtcagacca aggtctttac ccactttcag
     3121 gcgcagtgcg cccatcgcgt cggcaacaat tttcttgttg tcggcaccga agaaaatcat
     3181 atcgccatct tgcgcggcag tacgatccag gatggcttcg atgatttctg cattaaggaa
     3241 cttcgctacc gggctgttga taccttccag acctttcgcg cgttcgttaa ctttgatgta
     3301 agccagacct ttcgcgccgt agattttaac gaagttaccg tattcgtcga tctgcttacg
     3361 ggtcagcgat gcgccacccg gaacacgcag agcggcaacg cgacctttcg gatcgttcgc
     3421 cggacctgca aatactgcaa actcaacaga tctcagcaga tcagcaacgt cggtcagttc
     3481 catcgggtta cgcagatccg gtttatcaga accataacgg cgttcggctt ccgcaaaggt
     3541 cattactggg aaatcgccca gatccacgcc cttcacttcc agccacagat ggcgcaccag
     3601 cgcttccatc acttcacgca cttgcggcgc ggtcatgaaa gaagtttcca catcgatctg
     3661 agtaaattca ggctgacgat cagcgcgcag gtcttcgtca cggaagcatt taacgatctg
     3721 atagtagcgg tcaaagccgg acatcatcag cagctgtttg aacaactgtg gggattgcgg
     3781 cagtgcgtag aatttacctt tgtgcacacg agaaggcacc aggtagtcac gcgcgccttc
     3841 cggcgtggct ttggtcagca tcggagtttc gatgtcgagg aaaccgtggt catccataaa
     3901 acggcgcacc aggctggtga ttttagcgcg ggttttcagg cgctgagcca tttccggacg
     3961 acgcaggtcg aggtagcggt atttcagacg cgcttcttcg gtgttgacgt ggttagagtc
     4021 aagcggcaga acatctgcgc ggttgatgat agtcagcgag gacgccagca cttcgatttc
     4081 gccggtcgcc atatcgcggt taatattttt ttcgtcacgc gcacgtacgg tgcccgtgac
     4141 ctgaatgcag aactcattac gcagttcaga ggccagcttt aacgcgtccg cacgatccgg
     4201 atcgaaaaat acctgcacga taccttcgcg gtcgcgcata tcgatgaaga tcaggctacc
     4261 aagatcacga cgacggttga cccaaccaca cagagtcacc tgctgcccca cgtgggacaa
     4321 acggagctgt ccacaatatt ctgtacgcat gagatatccc ttaacttagc tgccggcgga
     4381 tgccccctgc tgcgcaggtg accaagtcgc agcgttagct gtatgtcaca actgaatgaa
     4441 aaaaggcggc tattatactg gaaattctgc cgcaccgtaa gagcctggcc cgcgctggaa
     4501 cgcctcgtta ccactttata tcgggcctga aatcagactc tacgccagtt tgctataaag
     4561 gtgttgcccg aactcataca aattaacaaa atttgtcgtt ccgccatcgg ctaatcgcat
     4621 taaggtgtga ggcacgattt tgttttttca ggagtcatca tgcttgaact taatgctaaa
     4681 accactgcac tggtggtgat cgatttacaa gaaggcatct taccttttgc cggtggtcca
     4741 catactgccg atgaggtagt taatcgcgcc gggaagctgg cggcgaaatt tcgcgccagc
     4801 ggtcagcccg tgtttctggt gcgcgttggc tggtctgccg attacgccga agcattaaaa
     4861 cagccggttg atgccccttc ccccgctaaa gtgttgcccg aaaactggtg gcaacatcca
     4921 gctgcattag gtgcaactga cagtgatatc gaaataatca aacgtcagtg gggtgcgttt
     4981 tacggtacgg atctggagtt gcaattacgc cgccgaggta tcgatacaat agtgttatgt
     5041 gggatctcga ccaatatcgg agttgaatcc accgcccgca atgcctggga acttggcttt
     5101 aatctggtga ttgccgaaga tgcctgtagt gccgctagcg ccgagcagca caataacagt
     5161 atcaaccata tctacccgcg catcgcccgt gtgcgtagcg tggaagagat cctcaacgcg
     5221 ttatgattta catcggtcta ccgcaatggt cgcatcctaa atgggtgcgg ttggggatca
     5281 ccagccttga agagtatgcc cgtcacttta actgcgtgga gggcaacacc acgctttacg
     5341 ccctgccgaa acccgaggtt gtcctgcgct ggcgtgaaca gaccacagat gacttccgct
     5401 tctgttttaa gtttccggcg accatttcgc atcaggcagc attacgacat tgcgatgatt
     5461 tagtgactga atttttgacc cgcatgtcac cgttggctcc gcgcattggg caatactggc
     5521 tgcaactgcc tgccacattc ggcccacggg agctgcctgc gctttggcat tttctcgatt
     5581 ctctccctgg cgaatttaat tatggtgtgg aagtccgcca tccacagttt ttcgccaaag
     5641 gggaagagga acaaacgctt aatcgcggtt tacatcagcg cggcgttaat caggtgattt
     5701 tagacagccg cccggttcat gcagcacgtc cacacagtga agctattcgc gacgctcaac
     5761 gaaaaaaacc taaagttccg gtacatgctg tactgacggc gacaaatccg ctgatccgtt
     5821 ttatcggtag tgatgatatg acgcaaaacc gggaattatt tcaggtctgg ttacaaaaat
     5881 tagcgcagtg gcatcagacc actacgcctt atcttttttt acatacgcca gatattgccc
     5941 aggccccgga actggtacat accctgtggg aagacttacg taaaacgctt ccagagatcg
     6001 gagcagttcc ggctattcca cagcaatctt ctcttttctg aatttgccac ctatcataga
     6061 caggtgccat cggccatttt aaagggagtt tgtatggtaa gcgcgctgta tgccgtttta
     6121 agtgcgttgt tattaatgaa gttctctttt gatgtcgttc gcctgcgaat gcagtaccgc
     6181 gttgcctatg gtgacggtgg ttttagcgaa ctgcaaagcg ctattcgcat tcatggtaac
     6241 gcggtggaat atattcccat cgcgattgtg ctgatgctgt ttatggaaat gaatggcgca
     6301 gaaacctgga tggtgcatat ttgcggcatc gttttgcttg ctggtcgtct gatgcattat
     6361 tacggttttc atcaccgtct gttccgctgg cgacgttccg gcatgagcgc cacctggtgt
     6421 gcgctgttgc tgatggtgct ggcgaatctt tggtatatgc cctgggagtt ggttttctcc
     6481 ctgcgttagc gcacaatacg ccactttctt tttcccggat ttttacgtta tgtctcaccg
     6541 cgacacgcta ttttctgccc ctatcgccag actgggcgac tggacctttg atgaacgggt
     6601 agctgaagtc ttcccggata tgatccagcg ttccgttccc ggctattcca atattatttc
     6661 catgattggt atgttagccg agcgcttcgt tcaacctggt acgcaggttt acgatctggg
     6721 ttgttctctg ggcgcggcga cgctctcggt gcgtcgcaac attcatcatg ataattgcaa
     6781 aattattgcc atcgacaact ccccggcgat gattgaacgc tgccgtcgtc atattgacgc
     6841 ctataaagcc cctacgccag tagacgtcat tgaaggtgat attcgcgata tcgccattga
     6901 aaacgcatcg atggtggtgc tgaattttac cctgcaattc ctggaacctt ccgagcgcca
     6961 ggcgttactg gataaaattt atcaagggct gaaccccggc ggcgcgctgg tgctttcgga
     7021 aaaattcagt ttcgaagatg ccaaagttgg tgaactgttg ttcaacatgc accacgactt
     7081 taaacgtgcc aacggttaca gcgaactgga gatcagccag aagcgcagca tgctggaaaa
     7141 cgtgatgctg accgattccg tggaaaccca taaagcacgc ctgcataaag ccggttttga
     7201 gcatagcgag ctgtggttcc agtgctttaa ctttggttca ctggtggcat taaaagcaga
     7261 ggacgctgca tgatcgactt tggtaacttt tattctctga ttgccaaaaa tcatctttca
     7321 cactggctcg aaacgctgcc cgcgcagatt gctaactggc agcgcgagca gcagcacggg
     7381 ttgtttaagc agtggtcaaa tgcggtggag tttctacctg aaattaaacc gtatcgtctt
     7441 gatttattgc atagcgttac ggcagaaagc gaagagccac tgagcgcagg gcaaattaaa
     7501 cgcattgaaa cgctgatgcg caacctgatg ccgtggcgca aagggccgtt ctcactgtat
     7561 ggcgtcaaca tcgataccga atggcgttcc gactggaaat gggatcgtgt tctgccccat
     7621 ctttctgatt taaccggacg caccattctg gatgtcggct gcggcagcgg ttatcacatg
     7681 tggcgcatga ttggcgcagg cgcgcacctc gcggtgggga tcgaccctac gcaactgttt
     7741 ctgtgccagt ttgaagcggt gcgtaaacta ctgggtaatg atcagcgcgc gcatttgtta
     7801 ccgttaggta ttgaacaact tccggcactg aaagcctttg ataccgtctt ttcgatgggc
     7861 gtgctttatc atcgtcgttc accgctggag catctctggc agttgaaaga tcaactggtc
     7921 aatgaaggtg aactggtgct tgaaacgctg gttattgatg gcgatgagaa cacggtgctg
     7981 gtgccgggcg atcgttacgc gcaaatgcgt aatgtctatt tcatcccttc cgcgctggcg
     8041 ctgaaaaact ggctgaagaa gtgtggtttt gtagatatcc gcattgcgga tgtgagcgtt
     8101 accactacag aagagcagcg acgcactgaa tggatggtta ccgagtctct gtccgatttc
     8161 ctcgacccac atgatccgag taagacagtt gaaggttatc ctgcgcctaa acgcgcggtg
     8221 ctgattgcgc gcaagccgta aaggtctggt attactgccg gatgcggcgt gaacgcctta
     8281 tccagcctac aaaaccttgc taattcaata tattgcaggg actatgtagg cctgataagc
     8341 atagcgcatc aggcagcttt atgtttgcat aacctcagcg cccgtttccg ggcgcttttc
     8401 acgtcttatg cctgaacagc tggctgatca aacgccgtta acttcggtgc gttacccgta
     8461 tatttttcga tatacaccag cgcagagtta ccggcacagg catttgccag ctgcgagctg
     8521 gggatatccg ccgttaacac gttcgcactg ccgtttttac acaagccatt ttccagatct
     8581 ggccatgcac cttcatgcac gcataccacg ccttttttga tcccgtcagt caccacagcg
     8641 cctgtcaaaa tctgaccacg tttgttccag acacggacca gatcgccatt cgcaatacca
     8701 aagcgagcag catcttcggt gtgaatagtg attggttcac gatcggcaac cgcatatttt
     8761 ttacgcagtt gcgcatagtt aagctgactg tgtaaacggt gtgccggatg tgcggtcaga
     8821 agctgtaact gcttctcgtc ggcggtacct ttccactcat caggcgcaag ccaggttggg
     8881 tgtgccgggc aatccttata gccaaatttt tccagcgttt tggagtaaat ctcaattttg
     8941 ccgcttggcg tacccagcgc atttttcacc ggatcggcgc ggaaatcacc ataacgaacg
     9001 tactgttcgt tcttctcgct gcggcgcatt tcgatcagtt tattttgctg ccagaaggca
     9061 ttaaacattg gcatagtgac gcgttgcgca cgggcacctt tctgagcggc atcatagaaa
     9121 aatttcagcc acgccatttc atctttacct tcggtataga tctcttttcc gcctggtttc
     9181 agtaattccg caagttcggc aaacacgtca aaatcgtttc gcgcttcaaa ttgcggagcg
     9241 acagcctgct tcatcggtac aatatgctgg ttgctgtaat caccggtcat cgtcaagtca
     9301 ttgcgctcaa acgatgtggt gatcggtaat acgatatctg catgtttagc agctgcggtc
     9361 cagtagcatt cagaaacgac gatcatctcc ggtttctgcc atgctttgat cagacgatta
     9421 gtatcctggt gatgggtaaa gttaccgcca cccgcccacc agatcatttt gatattcgga
     9481 tacgtttgtt ctttaccgtt atgctgatat ttgccgccag gattttccag cgcatcgacg
     9541 atacgggcaa caggaatcgc cgtcattccg ccatcatcag cggcctcgct ggcctgtccg
     9601 gcaatcgccg cagacatttc cggcagcacg ccgccaacac gcgtcgggtt gccaccgttg
     9661 gagtaatgat aagagaaacc aaaaccgccg cccggtgtac caatctgccc taacatcgcc
     9721 gccagcgtca ccagcatcca gtgtttctgt tcaccatatt gctggcgctg aattccccag
     9781 cctgccatca gcattgtgcg attcgccgcc atcagttcag cgagtttgac tatctgcgct
     9841 tccggtacgc cagtaatttc cgccgtccag gccgcgctct tcggagtatt atcgctctta
     9901 cctgtcagat actcttcaaa ctgcggatac ccggtggtgt atttttcaag gaacacttta
     9961 tcgtgtttgc cttgtgtcat cagggtatgc gcaatcccta acatcagtgc cacgtcggtg
    10021 cccatattcg gcgcgatcca ggtggcatta tagccaaaga actcgatggt ttcagagcgg
    10081 ataggatcaa tggcaatcac tggtttgcca gattttttca gctgatggaa gtattccagc
    10141 ccttgctcat cggtactgct ccaggcaatt tttaaggtat tcagcgggtt cattccccac
    10201 agcaccacaa cctggctgtt ttccagaatc agcggccagg aggtctgctg ttcatacacc
    10261 tctacagaac cgaccacatg cggcatgatc acctgtgctg ccccggttga ataatcgccg
    10321 ctatgcccgg aataaccgcc cgccaggttc atataacgtt gcagtaaggt ttgcgcttta
    10381 tgcaacacgc cagaagagcg ccagccgtaa gatccggcaa aaatggccga tggtccgtta
    10441 gctttacgaa tacgctcatg ttgctcatga atcagtttta atgcctgttc ccagctcacc
    10501 tgtacatagg tatcttcgcc acgacctttc gccggttgca gtggattgtc gagatagctt
    10561 tttctcacca tcggatgctg aatacgcgcc gtggtgtgta cctgatccgc cgccgtagac
    10621 tgtaaggagt tcggtatggt tttcgccagc gcgcctgtcg aagaaacaat cttgccgtcc
    10681 ttcacttcta cgttcatcgc tccccaacgt cccgcggtga ggattttacc gcccttctct
    10741 tctgcccatg cgggtagcgg tgctgccgat gtcaccacca gcgctccagc ggcaataccg
    10801 ctgtgtttaa taaattcacg tcttgttaat gtcataactt cctccctgat caacgaggat
    10861 cactgtttct cggtaatatc tttggcgttg tactggaaat accgcgttaa aatgtccagt
    10921 tcgttttcgc tcatgctggt tcgtgccccc attcctttgg caatggacgg ccacgcattg
    10981 acggtgtaat ggtcggcggc aataggggca tgacaaccag cgcaataggt atcgtcaagt
    11041 ttttcagcgt attgccatag cagtttacgg tccgctaatg cgggatcggt aagcgcaccc
    11101 tgtaaagacg cctgacgcca ttgattgccg tattcgtcag cctgccattc cccgtttacc
    11161 gtgagcgcct tgataccttc ttcacttaat gtggctagcg ccagccgttg acctgccgcc
    11221 aggtagagcg tgttttcact gccctgcatt tgataaccct gcaacagaac gatcggctgt
    11281 ttgccactgg catcaacgac ggtgagatcg gtaccaggat tcacggtagc cagctcgcct
    11341 atgcgagaag ttttgaaagg ataaatatgt gcgccattag tcactgaagt agcggcctga
    11401 ctttccagct catgcgccgc gttgtcatcc atttttattt ctggcggaaa atgggcaatg
    11461 cctttatgac aatcgataca ggtttcgccg cccttttgtg ctttgttatg cattttctgc
    11521 gcagattcac tttgcgaggc aatatccatg gcatcaaaag aatggcaact acggcacgtt
    11581 gcagagtcag ttgcttttaa ttctttccat actgtttcgg ccatttcctg gcgatgagct
    11641 tcgaacttat cgtcactgtc tattttgccg ctaacaaatt catgataaat atctttagat
    11701 gcttttaatt tagcgaataa ataatccatc cctgactttg gaatatggca atcggcacat
    11761 tccgcacgta tccctttctg gttcgaaaag tggacagttc cctgatattc ctcaaaaggt
    11821 ttactcatcg agtggcagga aaggcaaaat gctgtatccg acgttttatg taaggctttt
    11881 tgcgccatca agaacccgcc accaccaacc acaaccgcta tcagcaaaaa caataaccca
    11941 atgcgttttt tccctcgcat agattcctct aattaaattc cagaacaccg aaatcattgt
    12001 ataaataaat acacaacgaa ttgactactt gcttaattcg gcacaacact gcctgaaata
    12061 atcgctaaag aatatgattt attacaatgt aatcattaat tgctaa
//
LOCUS       DAZFAB010000122        12095 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZFAB010000122 DAZFAB010000000
VERSION     DAZFAB010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12095
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.122"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..2384)
                     /locus_tag="ACHJWA_004012"
     CDS             complement(<1..2384)
                     /locus_tag="ACHJWA_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO4310512.1"
                     /translation="MSKIFICAAIPDEQAIKEDSAVAVATAIEAGDERRARAKFHWQF
                     LEQFPAAQDCAYKFIVCEDKPGIPRPALDSWDTEYMQENRWDEESASFVPVEPESDPM
                     NVNFDKLSPEVQNAVLVKFDTCENITVDMVISAQELLQEDMATFGGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCEPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLTRA
                     RVNQQNTENAAEKTGAVTVAIRREYKQTWKTLDNELACALWPGDVDAGNIDGTIHRWA
                     TNEVIDKDREDWKRISASMRKQPEALGYDRQTIFGLVRERPIDIHKDPVALNKYISEY
                     LTTKGVFEHEETDQSSTDALQPSAAQTAPVETAESDTQKNEILVEAEPSVEREGPFYF
                     VFTDKGGEKYGRANKLSGLDKALAAGGTEISKEEYFARKNGTYTGLPQNVDTAEDSEQ
                     SEPVKVTADEVNKIMQAANISQPDADKLLAASRGEFVEGISDPNDPKWVKGIQSRDAE
                     DQNQPNVKQNEPEAEQNSPDTQQNGPEEQQPEPAVQQELEKVCTACGQTGGGNCPDCG
                     AVMGNATYLETFDEENQAEAQKNDPEEMEGTEHLHKENTGSDQYHASDNKTGETANPL
                     IKVNGHHEISSTSRLWHHLMIDLETMGKNPDAPINSIAGKFFDPATGEMGPEFSKTID
                     LETAGGVIDRDTIKWWLKQSREAQSSILTDEITLDDALLQFREFIDENSGEFFVQVWG
                     NGATFDNVILRRSYERQGIPCPWRYTNDRDVRTMVALGLVMDFDARTTIPFEGER"
     gene            complement(2478..2669)
                     /locus_tag="ACHJWA_004013"
     CDS             complement(2478..2669)
                     /locus_tag="ACHJWA_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO4310513.1"
                     /translation="MNTTFALVLTVYLVSGESLELVTGLYGSMKECMAAAAEQKIPGN
                     CYPVDKTTHTNNNEIPAGL"
     gene            complement(2666..2854)
                     /gene="dicB"
                     /locus_tag="ACHJWA_004014"
     CDS             complement(2666..2854)
                     /gene="dicB"
                     /locus_tag="ACHJWA_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO4310514.1"
                     /translation="MEKLSCNASTSELRFEIGVITGDKTFIEDAIKQRKLEQDLLNEV
                     CIPSMLARLDLLQKGYKQ"
     gene            3428..3613
                     /locus_tag="ACHJWA_004015"
     CDS             3428..3613
                     /locus_tag="ACHJWA_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310515.1"
                     /translation="MPNKPCPACNALSGFLEKGGYYIFNCPEHVEFHISKLDSIITKP
                     NQYQSDLLNKELNAARD"
     gene            complement(3800..4189)
                     /locus_tag="ACHJWA_004016"
     CDS             complement(3800..4189)
                     /locus_tag="ACHJWA_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310516.1"
                     /translation="MEFKDLLKEIQEIAAHALHQRLNEVELESATKKYIDNMARNVRD
                     AFTGLYSVSVTNNQNTEETAKRIASVMGFHVEEKYSKKEFWKTTKKLQSENCHLLRQS
                     LLSMRKVIQMTQDYRNCSHYLKNSELS"
     gene            complement(4201..4329)
                     /gene="ydfB"
                     /locus_tag="ACHJWA_004017"
     CDS             complement(4201..4329)
                     /gene="ydfB"
                     /locus_tag="ACHJWA_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfB"
                     /protein_id="HFO4310517.1"
                     /translation="MDFDTIMEKAYEEYFEGLAEGEEALSFSEFKQALSSSAKSND"
     gene            complement(4331..4486)
                     /gene="ydfA"
                     /locus_tag="ACHJWA_004018"
     CDS             complement(4331..4486)
                     /gene="ydfA"
                     /locus_tag="ACHJWA_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO4310518.1"
                     /translation="MDTIDLGNNESLVYGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            complement(4764..5051)
                     /locus_tag="ACHJWA_004019"
     CDS             complement(4764..5051)
                     /locus_tag="ACHJWA_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO4310519.1"
                     /translation="MLPILWLPSARDDLRQIITYIAKENPPAARRLKIRIETSVLPLS
                     EHPYLYPPSERVSGLREIVTHPNYIILYRVAASSIEIVSVTHSRRQFPFSI"
     gene            complement(5051..5242)
                     /locus_tag="ACHJWA_004020"
     CDS             complement(5051..5242)
                     /locus_tag="ACHJWA_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFO4310520.1"
                     /translation="MGTALSPIVSEFETTEQENSYNEWLRTKVTSSLADTRPAIPHDE
                     VMAEMENLIAQIAVTNKSE"
     gene            complement(5270..5671)
                     /locus_tag="ACHJWA_004021"
     CDS             complement(5270..5671)
                     /locus_tag="ACHJWA_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4310521.1"
                     /translation="MMSALEVSMYRISKLLQETGWSQAELARRIGVTQQTVQQWVSGK
                     ATPKASSLDKLVEVSGHPLHWFLLPPEECEQIFTPDTMKIGPRQRELLQAFSAFPEED
                     QEKMLQEIKDKKKSMEETIARWLAAQKSRRA"
     gene            5780..6052
                     /locus_tag="ACHJWA_004022"
     CDS             5780..6052
                     /locus_tag="ACHJWA_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO4310522.1"
                     /translation="MLDSTREKIRQKYTQAEIGRYMGVAQQTVWQWFSFGVPPKQVIP
                     LCQLMKWEVTPHEIRPDIYPNPTDGLPVGCKVNTSNAPELIHENQA"
     gene            6036..6461
                     /locus_tag="ACHJWA_004023"
     CDS             6036..6461
                     /locus_tag="ACHJWA_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO4310523.1"
                     /translation="MKIKHEHIRMAMNAWAHPDGEKVPAAKITKAYFELGMTFPELYD
                     DSHPEAMARNTQKIFRWVEKDTPDAVKKIQALLPAIEKAMPPPLVARMRSHSSAYFRE
                     LVETKERLVKDIDDFVASAIVLFDQMNRGGPAGNTLAVH"
     gene            complement(6668..7123)
                     /locus_tag="ACHJWA_004024"
     CDS             complement(6668..7123)
                     /locus_tag="ACHJWA_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310524.1"
                     /translation="MYFLKSLYQAHVLNVAATNRWCNSPEMLPDYRAWLRAETYLRLD
                     ILISELQKETASIHNLQGIDAVRILVSRHSALSIIEVRHLSFSELIFLLQPALESANI
                     PPEVIQYPPHVDEQLQDVPYNQRAGLTPCSEAEWDHSLLKKYQDLYNPQ"
     gene            7202..8293
                     /locus_tag="ACHJWA_004025"
     CDS             7202..8293
                     /locus_tag="ACHJWA_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO4310525.1"
                     /translation="MRDYAKVSPRFWLGETGRELRKAGAEAQVVAFYLMTSPHANMLG
                     LYYLPVLYLAHETGLGLEGASKGLKRAVEAGFCSYDHDAEMVWVHEMAAWQVGETLKP
                     GDNRCAGVRNEYASLPENAFLSVFYDRYKTDFHLDVRRNNSRNSVRGFEGAFKGLRSQ
                     EQEQEQEKEQEQDKNTMVHGKKNTTNQAGDVQTVNPGQPAGTTPEADSGAVQQVMTAG
                     SEQSHQLQQPEADSAIQREADRVVPESTGQSVGRVDYPDVFEQVWREYPLRAGANPKK
                     SAFSAWKARLREGVPPETMLDGVRRYARYLAATGKAGTEFVQRATTFFGPDRNFENPW
                     LLPVSGTNNQRCVNHISEPDTEIPPGFRG"
     gene            8300..9046
                     /locus_tag="ACHJWA_004026"
     CDS             8300..9046
                     /locus_tag="ACHJWA_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO4310526.1"
                     /translation="MKNIAAAGVLERIRRLAPQASVPPYRTVEEWREWQLAEGRKRSE
                     EINRQNHQLRVEKILNRSGIQPLHSKCSFANYQVQNDGQKYALSQAKSIADELMTGCT
                     NFVFSGKTGTGKNHLAAAMGNRLMAKGRSVIIVTVSDVMSVLHDSYDNGKSGEKFLQE
                     LCSVDLLVLDEIGVQRETKNEQVVLHQIIDRRTASLCSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGGRWVTFNWDSWRPNVSNMRVVK"
     gene            9068..9838
                     /locus_tag="ACHJWA_004027"
     CDS             9068..9838
                     /locus_tag="ACHJWA_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO4310527.1"
                     /translation="METVSDALKALKKASSHVVAARLGISREEAVNELWELKINGVVD
                     KTGHTWFLAGEGESRVTEERPVKSEAQDMLTGEVEQKVTADMMIEFIGQDGAKTCEEL
                     AGKFGVSTRKVASTLAVVTATGRLARVNQNGKFRYCMPGDNLPAEPKAALVTESDGKA
                     FPQPAGAALPVREAATQEEIKTETVADIVQPLPSFTETQADELIFPSLRRANLALRRA
                     KSDVQKWERVCAALRELNKHRDIVRQITDSSRRVVSEK"
     gene            9854..10267
                     /locus_tag="ACHJWA_004028"
     CDS             9854..10267
                     /locus_tag="ACHJWA_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO4310528.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRRSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPVVVDPDLIWSLPDG
                     EIRRYDRQLNIICSECRNSEVMQRVLIFYTGVMME"
     gene            complement(10619..11392)
                     /locus_tag="ACHJWA_004029"
     CDS             complement(10619..11392)
                     /locus_tag="ACHJWA_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO4310529.1"
                     /translation="MTKHMDIHCQTRIFNSINHDTKYKICIFTPKNIKKNNATLYILD
                     GNSANNYISDILPVIDALPNPPVLVTLGYESWNNLSIHRRAYDYTPDGENAIVDNSKP
                     AWIYFTGGGSQSFRELLLTQIMPWVSTIAPNSSRIGIWGHSLGAIFVLDCLKNNSCFN
                     YYYISAPSLLWQNERIIKIIKDDISESKHTKSICLLNGNLSLDYSASLYPEAIKAESV
                     LRNILTEKYSNFSIVQFPELNHQETFSAALWNSIIHFSI"
     gene            11997..>12095
                     /gene="hokD"
                     /locus_tag="ACHJWA_004030"
     CDS             11997..>12095
                     /gene="hokD"
                     /locus_tag="ACHJWA_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO4310530.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVR"
BASE COUNT         3153 a         2622 c         2987 g         3333 t
ORIGIN      
        1 cgttcacctt cgaatggaat agtcgttctt gcgtcgaaat ccatcaccag tcccagagca
       61 accatcgttc ttacatcgcg atcattggtg taacgccacg ggcaggggat cccctgccgt
      121 tcatatgaac ggcgtaaaat cacgttgtcg aaagttgcac cgttacccca gacctgaaca
      181 aaaaattcac cggagttttc gtcgataaat tcccggaatt gcagcagtgc atcatccaac
      241 gtgatttcat cggtcagaat ggaggattgt gcttcgcgtg actgtttcag ccaccactta
      301 atggtgtccc gatcgatgac cccacctgcg gtttccagat cgatagtttt gctgaattct
      361 ggccccatct ctccggttgc cggatcaaaa aacttaccgg ctatagagtt tattggcgca
      421 tcaggatttt ttcccattgt ttcaaggtca atcatcagat ggtgccacaa cctgctggtg
      481 gatgagattt catgatgacc gttcacttta attaagggat ttgctgtttc gccagtttta
      541 ttatcgctgg cgtgatactg atcgctgcca gtgttctcct tgtgcagatg ttcagtgcct
      601 tccatttcct ccggatcatt tttctgagct tcagcctgat tctcttcatc gaatgtttcc
      661 aggtaggttg cgttccccat caccgcacca cagtcaggac agttgccgcc accggtctga
      721 ccgcatgcgg tgcaaacttt ttccagttcc tgttgcactg ctggttctgg ttgttgttct
      781 tctggcccgt tttgttgcgt atccgggctg ttttgttccg cttctggctc attttgtttc
      841 acgttgggct gattctggtc ctcagcgtcg cgactctgga tccctttcac ccatttcgga
      901 tcattcgggt cgctaatccc ttcaacaaat tcaccacgtg atgcagcaag caatttatcg
      961 gcgtcaggct ggctgatatt ggctgcctgc ataattttgt ttacttcgtc agcggtaact
     1021 tttaccggct ctgattgttc ggaatcttca gcggtatcca cattttgcgg taagcccgtg
     1081 tatgtgccat tttttcgggc aaaatattct tcttttgaga tttcggtacc gccggcagcc
     1141 agcgccttgt ccagaccaga aagtttgttt gccctgccgt atttttcccc gcccttatcg
     1201 gtaaagacga aataaaatgg tccttcacgc tctacagatg gttcagcttc caccaggatt
     1261 tcattttttt gagtatcgga ttctgccgtc tccactggag cagtttgtgc tgctgacggc
     1321 tggagagcat cagtagagct ctggtctgtt tcttcatgtt caaacacgcc ctttgtcgtc
     1381 aggtattcac tgatatattt gttcagtgca acgggatctt tgtgaatatc gatcggacgt
     1441 tcgcgaacaa ggccaaaaat agtctgacgg tcatagccaa gtgcttcggg ctgtttgcgc
     1501 attgatgctg agatacgctt ccagtcttcg cgatccttgt cgataacctc atttgtcgcc
     1561 cagcgatgga tggtaccgtc aatgtttcct gcatccacat caccgggcca gagggcgcag
     1621 gccagttcat tgtcgagtgt tttccatgtc tgtttgtatt cgcggcgaat ggcaacagtg
     1681 acagccccgg ttttttcagc agcgttttca gtgttctgtt ggttgactct ggcgcgggtg
     1741 agatcaacaa cagacgtgta ttttccggtt tccttgcgtt caccttcgcg acgttttttc
     1801 cagatgcgca tctctgcctg aatttcaggc catttagcgc caggctcaca tttatgctta
     1861 acccacccga tggcatgcag tttaagttca ggatacatgg cgttaacttc tggcattttc
     1921 atcaacgctt caacgatatg tccgccgaat gttgccatgt cttcctgtag taattcctgt
     1981 gcgctaatca ccatatcaac ggtgatgttt tcgcatgtgt cgaatttaac caggactgcg
     2041 ttctgtactt caggggacag cttgtcaaaa ttgacgttca tcggatcgga ttctggttcg
     2101 accggtacaa aggaagctga ctcctcatcc cagcggtttt cctgcatata ttcggtatcc
     2161 caggaatcga gggcagggcg gggtatgccg ggtttatcct cgcagacaat aaatttataa
     2221 gcgcagtcct gagctgccgg gaattgctcc agaaattgcc agtgaaattt tgcgcgtgcg
     2281 cgacgctcat caccggcttc aatggcagtg gccaccgcaa cagcgctatc ttcttttatg
     2341 gcctgttcgt caggaatagc agcgcaaata aagattttac tcattgtgtt ttaacctcat
     2401 tacagattta agggtgaaca aatccctgcc attgctggca tataaaaatg aaaccggata
     2461 ttaattacgg tgctgtttta aagtcctgcc ggtatttcgt tattattagt gtgagtagtt
     2521 ttatctaccg gataacagtt accgggaatt ttttgttctg ctgctgcagc catgcattct
     2581 ttcattgaac cgtataagcc agtcaccagc tcaagagatt cgccggaaac aagataaact
     2641 gtcagaacga gtgcaaatgt tgtattcatt gtttatatcc tttttgcagc aggtccagac
     2701 gagccagcat tgaaggaatg catacttcat ttaacaggtc ctgctcgagt tttctctgct
     2761 taatggcgtc ttcaataaat gttttgtctc cagtgataac gccaatttcg aaacgaagtt
     2821 cagacgtgct ggcattacat gataactttt ccattatcgc gtcctcaaca atgaattttg
     2881 tgatgcggtg cctggtgcct ccaggtgacg ttaaccagtt aacaattaac gccggataca
     2941 gagcattctc gttactcagc aaatgaccgc tttaccgatt taactgttcc gcgtgcgctg
     3001 agccgcattc accgcatcac aaaattcact tttaaaaaag ggcggcagag cagttacgga
     3061 gtaaaactga taccgccaaa tgtcaccaga atattgataa cagagggcgt tgcagcgggg
     3121 ttgtcactta agcgtatggt caacctgaca acccggtgtc ctcaacgggg aaagaataac
     3181 cccgccatac ttaccgccgc gccatttcgc ggattgccac aaccggaagc gcacggtcga
     3241 attaaattta acgacggctc taagggagaa ggacttcgcc gtgcactttc gtgttatgcc
     3301 ctgacttttc agggatatat cctttcagta aactgtcagt gccggattct tatctgtgtc
     3361 cggcgcacga ccacacgtag cagcgtgttg gtctccattt ttaattcagc ccttaatgga
     3421 ggataaaatg ccaaacaaac catgccctgc ctgtaacgct ctttctggct ttttagagaa
     3481 gggcggttat tacattttta actgtccaga acatgttgaa tttcatatat caaaacttga
     3541 cagtatcatt acaaagccaa atcagtatca gtctgattta ctgaataaag agttaaacgc
     3601 cgcccgagat taagggcgta gtggcccatt aaaaatattg caaagaccaa ctattgttga
     3661 actggattaa accagtaatt ctctattgct cttattgtta ccatcggatg attgccgggg
     3721 gttattttaa gttcgggagg aaaaggctct ccatcagcag ccatcattga gtatgtgaaa
     3781 tccgccgacg cagtcccttt catgataatt ccgaattttt caagtaatga gagcagttcc
     3841 ggtaatcctg tgtcatctga ataactttcc tcatagaaag aagcgattgc cgcaggagat
     3901 gacagttctc gctctgcaat ttttttgtag ttttccagaa ttcttttttt gaatactttt
     3961 cctcgacatg aaaacccatc accgaggcaa tccgctttgc agtttcttca gtattctggt
     4021 tgtttgttac cgaaacagag tacaatccgg taaacgcatc gcgcacatta cgagccatat
     4081 tatcaatgta tttcttcgtt gcagattcta attcaacttc gttcagacgt tggtgaagtg
     4141 catgtgctgc aatttcctgg atttctttta gtaaatcttt aaattccatc tttaactccg
     4201 ttagtcgtta gattttgccg aactggaaag cgcctgttta aactcactga agctgagagc
     4261 ttcctcgcct tcggcaaggc cttcgaagta ttcttcgtaa gccttttcca tgattgtgtc
     4321 gaaatccata tcactcacct gagtttcttt ccagccagcg acgggcacca ttttcggttt
     4381 taaacgtttt gcttttggta tacgtcatcg cggtgaatgt accgtcctgg ttgggaaaca
     4441 cgccgtacac cagagattcg ttgttgccaa gatcgatagt atccatgttg acctcatttc
     4501 cccttaacgc cgggtagcgg aacaaaaacc tgctgcatag ttattaaagt tgaaccctgc
     4561 cgtcatgttc atacgcctcg ggctggctac ttaccccctg accactgctt ggtaactcga
     4621 agtattgccc ggcgttctgt ggggcggggt gggtggttgg tgtatgtaat ctacaattaa
     4681 aaactgtttt tgtgtcaaca gtttttaatt gttgttttgg gcaaaaaaat ccctcgaaag
     4741 agggagtatg aaaattgttc aactcagata gagaagggaa attgtcgccg agaatgtgtc
     4801 acgcttacaa tctcaatgct tgaagcagct actctgtaca ggattatgta gttagggtgg
     4861 gtcacgatct ctctcaatcc agaaacccgt tcgcttggtg gatataagta cggatgctca
     4921 gatagaggta ataccgatgt ttcaatgcgt atttttagtc tacgtgctgc cggtgggttc
     4981 tccttggcga tgtaagttat gatctggcgc aaatcatcgc gagcagacgg tagccataaa
     5041 atgggtaaca ttactcgctc ttgttagtta cagcaatttg agcaataaga ttttccattt
     5101 cagccattac ctcgtcatgt ggaattgcgg ggcgagtgtc tgcaaggctt gacgttactt
     5161 tagtgcgcaa ccattcgttg taactgtttt cttgttcggt agtttcgaat tctgaaacta
     5221 tcggagaaag ggctgtaccc atggcataac tcctcttctt gtactgtggt cacgcccggc
     5281 ggcttttttg tgccgccagc cacctagcaa tggtttcttc cattgatttt ttcttgtctt
     5341 tgatttcttg aagcattttt tcttggtctt cctcaggaaa cgcactaaaa gcttggagca
     5401 gttcgcgttg gcgaggacca attttcatcg tgtcaggggt gaaaatttgc tcacactctt
     5461 caggaggcaa taaaaaccaa tgcaatggat gccctgaaac ctcaaccagt ttatccaaac
     5521 ttgaggcttt aggtgtagcc ttaccgctga cccattgttg aacagtttgt tgtgtcacac
     5581 caattctacg ggcaagctca gcctggctcc atccagtttc ctgaagaagc ttgctgattc
     5641 tgtacataga tacttctagg gcgctcatca ttattcaatt ttacaggtaa atactgttaa
     5701 aagcatcaca ataaaaaact gttgattgca tacagttttt tattgtaggc tttgcttata
     5761 gtttttagag gagggcaaaa tgctagatag cactcgcgaa aaaattaggc agaaatacac
     5821 tcaggctgaa ataggtcgtt atatgggggt cgctcaacag actgtttggc aatggtttag
     5881 ctttggcgtt cccccaaagc aggtaattcc gttatgccaa ctaatgaagt gggaagttac
     5941 cccgcatgaa atccgcccag atatttatcc taacccaacc gacggtttac ctgttggatg
     6001 taaggttaac acatcaaatg cgccggagtt gattcatgaa aatcaagcat gaacacatcc
     6061 gcatggcgat gaatgcctgg gcgcatccgg acggcgaaaa agtaccggct gcgaaaatta
     6121 ccaaagcgta tttcgagctg ggaatgacgt tcccggaact gtatgacgac agccatccgg
     6181 aagccatggc tcgcaatact cagaaaattt tccgctgggt ggagaaagac actcctgatg
     6241 cggttaaaaa aattcaggcg ttgttaccag cgatcgaaaa agcgatgccg cctccgctgg
     6301 tggcccgaat gcgcagccac agttccgctt attttcggga gttggtagag acgaaggaac
     6361 ggctggtgaa agatattgat gatttcgttg catcagcgat cgttctgttc gatcagatga
     6421 atcgtggtgg cccggcagga aacactctgg ctgtgcatta attgggtaat aaatatgagt
     6481 aatgacaaaa aattgacact gagcgtttac gaaaacagtc cgcacatctg gcgtggcggt
     6541 ttatctgatg tggagctggc agagtggttg atacataaag ctaatgcgct gctctggcgt
     6601 ttgtcagcca gagaacagcg caaggaaacc agaataaagc tggctgatgc agaagcgtgt
     6661 gccgggctta ttgaggatta tacaaatctt ggtatttctt cagcagagag tgatcccatt
     6721 cagcctctga gcagggagtc aatccagcac gctggttgta tggcacatct tgtaactgct
     6781 cgtcaacatg aggtgggtat tggatcactt ccggcgggat attcgctgat tccagagctg
     6841 gttgaagcaa gaaaatcagt tcagaaaaag agagatgacg cacttcaatt attgagagag
     6901 cactatggcg cgataccaga atgcgaacag cgtcgatacc ctgaaggtta tgaatggatg
     6961 cagtctcttt ttgaagttcg ctaatcaata tgtcgagacg aaggtatgtt tcggcgcgca
     7021 gccaggctct gtaatccggg agcatttcgg ggctgttaca ccagcggttt gttgctgcaa
     7081 catttaatac atgagcctga taaaggcttt tcaaaaaata catgtcgaac ctcctctggt
     7141 tctgtcgatt gggaaccaca gattatatcc ggaggaaggt tcggcaccag atgaggtagc
     7201 catgcgtgat tacgcaaaag tttctccgcg attctggctg ggagaaacgg ggagagaact
     7261 tagaaaggcg ggtgcagaag cgcaagttgt tgctttttac ctgatgacat cccctcacgc
     7321 aaatatgctg ggtttgtatt acctgccagt tttatacctt gctcatgaaa ccgggcttgg
     7381 tctggaaggg gcttcaaagg ggcttaaaag ggctgttgaa gctggttttt gtagctatga
     7441 ccatgatgca gagatggtct gggtccatga aatggcagcc tggcaggttg gggaaacgtt
     7501 gaagcctggc gataaccgtt gtgcaggtgt caggaatgag tatgcatcat tacctgaaaa
     7561 cgcttttctg tcagtgtttt acgacagata taaaacggat ttccatctgg atgtgaggcg
     7621 gaataatagc cgaaattcgg taaggggctt cgaaggggct tttaaggggc ttcgaagcca
     7681 agaacaggaa caggagcagg agaaagaaca ggaacaggac aaaaacacta tggttcatgg
     7741 caaaaaaaac accacgaacc aggcagggga tgttcagacc gtcaatcctg gtcagccagc
     7801 aggcacgaca ccggaagccg attcgggcgc tgtgcagcag gtgatgaccg cagggtcgga
     7861 gcaatcacac caactgcagc agcctgaagc cgattccgcc attcagcggg aagccgatcg
     7921 ggtagtcccg gaaagcaccg ggcagtctgt gggacgagtg gattatccgg atgtgttcga
     7981 acaggtctgg cgggaatacc cgttgcgtgc tggggcaaac ccgaagaaat ccgctttcag
     8041 tgcctggaag gccagattgc gcgagggggt gccaccagag accatgctgg atggtgtgag
     8101 gcgttacgcg agatacctgg cggcgaccgg gaaagcggga acggaatttg ttcagcgagc
     8161 gacgacgttt tttgggccgg accggaattt tgaaaacccc tggttgctcc cggtaagcgg
     8221 cacgaacaac cagcgttgtg tgaatcatat ttctgaaccg gataccgaaa ttccgccggg
     8281 attcaggggg tgatgtggca tgaaaaacat tgcggcagcc ggggttcttg aacgtattcg
     8341 cagacttgca ccacaggcgt cggttccacc gtaccggacg gtggaggagt ggcgggaatg
     8401 gcaacttgct gaaggacgaa aacgcagcga ggagattaac cgccagaatc accagttgcg
     8461 ggtggaaaaa atcctgaatc gttcgggcat ccagcctctg cacagcaaat gctcgtttgc
     8521 gaattatcag gtgcagaacg acgggcaaaa atacgcgctg agccaggcca aatccatagc
     8581 tgacgaactg atgaccgggt gcacgaattt tgtgttcagc ggtaaaaccg gcaccgggaa
     8641 aaatcacctt gcagcggcga tgggtaaccg gctgatggcg aaggggcgca gcgtgattat
     8701 cgtcaccgtg tctgatgtca tgagcgtgtt gcatgacagc tacgacaacg gcaaatccgg
     8761 ggaaaaattt ttacaggagc tttgcagtgt tgatttgctg gtcctggatg aaataggcgt
     8821 tcagcgggag acgaaaaacg agcaggtggt attacaccag ataattgatc gccggacagc
     8881 atcactgtgc agtgtcggga tgttaacaaa cctgaatcat gccgcaatga gtacacttct
     8941 tggtgagagg attatggacc gcatgaccat gaacggtggt cgatgggtga cgtttaactg
     9001 ggatagctgg cgtccaaatg tcagcaatat gagggttgtg aagtaatttt gtccggagga
     9061 aattttaatg gaaaccgtat ctgacgcact gaaagcactg aaaaaagcct cttcacatgt
     9121 ggtggcagct cgccttggaa tcagtcgtga agaggctgtc aacgagctgt gggaactcaa
     9181 aataaatggc gtcgttgata aaactggtca cacctggttt ctggctggcg aaggtgaatc
     9241 ccgggtaacc gaagagcggc cagtaaaatc tgaagcacag gatatgctga ccggggaggt
     9301 cgaacaaaaa gttaccgcag acatgatgat tgagtttatc ggtcaggatg gggctaaaac
     9361 gtgtgaggaa ctggcgggta agttcggtgt cagtactcgc aaggttgctt ccacgctggc
     9421 ggtggtaacc gcaacggggc ggctggcacg cgttaatcag aacggtaaat ttcgttactg
     9481 catgccgggc gataatttac cagcagagcc gaaagccgcg ctggtaacgg aaagtgatgg
     9541 taaggccttt cctcagccag caggtgctgc gttaccagtc cgggaagccg caacacagga
     9601 agaaattaaa acagaaactg tggcggacat tgtgcagccg ttgccatcgt ttaccgaaac
     9661 gcaagcagat gagctgattt ttccgtccct tcgcagggca aacctggcgc tgcgcagggc
     9721 gaaaagtgat gttcagaagt gggagcgagt ctgcgccgcg ctgcgggagc tgaacaagca
     9781 ccgggatatt gttcgacaga ttactgattc ttcccgccgt gttgtatcgg aaaagtgatt
     9841 gccggaggcg cttatggcaa aagtatttac acaggaagag cgggaaaaaa ttaaagggca
     9901 ggttgttgag ctagtacgcc ggagtgggcg cgagacgtta cggcaactgg aagccaagac
     9961 aggtgcgaca agatatctga tgagcgttct tgccagagag ctggttgcca gtggcgatgt
    10021 atacaactct ggttacgggt tattcccatc tgaacaggct cgtaaggact ggcaaaacgc
    10081 ccgcaaaaaa ctatcgaggg caaaactgaa gaaaccggtt gtggttgatc ctgaccttat
    10141 ctggtcatta ccagacggag aaatacgccg ctatgacagg caattgaaca taatttgtag
    10201 tgagtgccgt aacagtgaag tgatgcagcg agttttgata ttttacacag gagtaatgat
    10261 ggaatagtga aaataacatg aaacctttgg attctggtgt ttcagtggat gggaaaagag
    10321 cagatacata gaaaatgaat agcaataatt cacagtctgg atgttgtttg tatgcttata
    10381 aaatgatcct agtatgcatt gccggagttg aatcagctcc aatgaagaaa ctgagaggag
    10441 tatttagaag atagctaaat ggatgtaatt attagtatct aaaaaatagt atttttgaaa
    10501 tgggtctaag aacacaacaa tgtacaaatg agaagttatc ttttaatatg ttcaaagacc
    10561 cattaggatt gatatcagtt atgttagcta gattgataaa gtgattacat attttctgtt
    10621 atatgctgaa atgaataatg ctattccaga gtgcagcaga gaaagtctcc tgatgattga
    10681 gctctggaaa ttgtacaata gaaaaattac tgtatttttc tgtcaaaata tttctcaaaa
    10741 cagattccgc tttgatggct tcaggatata aagatgcgga gtaatcaagg ctaagatttc
    10801 cgttaagcaa gcatatactt ttagtgtgct ttgattctga tatatcatct ttaattatct
    10861 tgatgattct ttcattctgc catagaagtg atggtgcaga tatataataa tagttaaagc
    10921 atgaattatt ttttaagcaa tcaagcacaa aaatagcacc tagcgagtgc ccccatatac
    10981 cgattctgga actgttcgga gcaatagtac taacccatgg catgatctga gttagtaata
    11041 attctcggaa agactggctt cctccaccag taaagtagat ccatgctggt ttagagttat
    11101 caacaatagc attttcacca tcaggagtat aatcgtaagc acggcgatga atgctaaggt
    11161 tattccaaga ttcataacca agtgtaacta acactggtgg attgggtagt gcatcaataa
    11221 caggcagaat atcagaaatg taattgttgg cactattccc atcaagtata taaagagttg
    11281 cattattttt tttaatgttt tttggagtaa aaatacatat tttatatttg gtgtcatggt
    11341 taattgagtt aaatatgcgt gtttgacaat gaatgtccat atgtttagtc atgcgttctc
    11401 cccatgagca ttaaaggatg cttaagatat gctatgtcac tgataaaaaa tttgttgtgt
    11461 gtgtttagtt attttatcta cctccattat gatttaagac tgatatatgt tatcagcaaa
    11521 ggtacatcgt tattatttta cctgtcaata catttgatat tgattatcgt ttacatcgct
    11581 tgcttaggaa attgaaggca ggtaactatc atgtcaatga actaacccac agtctaacgt
    11641 acttctttcc ccaatcacca acaaccaaat tcctgttatc accactggga ttttggcgca
    11701 acttcatggt gcagaggtaa atagcatcaa gcaaaatctc tttttccgtt gttgtcctta
    11761 tgggacgtct gtctttctga ccgatttcat attggcgagg taatgggaag ttaagtagaa
    11821 tagctgcggg tgcttgaggc tatctgcctc gggcatgaac accaacggca gatagagaaa
    11881 agccccagtt aacattacgc gtcctgcaag acgcctaaca ttaatctgag gctcaatcta
    11941 tgaacggcaa atctaggtta gcctcttacg tgccgaaagg caaggagaag caggctatga
    12001 agcagcaaaa ggcgatgtta atcgccctga tcgtcatctg tttaaccgtc atagtgacgg
    12061 cactggtaac gaggaaagac ctctgcgagg tacga
//
LOCUS       DAZFAB010000123        12091 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZFAB010000123 DAZFAB010000000
VERSION     DAZFAB010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12091
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.123"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            1..2114
                     /locus_tag="ACHJWA_004031"
                     /pseudo
     CDS             1..2114
                     /locus_tag="ACHJWA_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
     gene            2451..3428
                     /gene="glaH"
                     /locus_tag="ACHJWA_004032"
     CDS             2451..3428
                     /gene="glaH"
                     /locus_tag="ACHJWA_004032"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFO4310531.1"
                     /translation="MNALTAVQNNAVDSDQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGV
                     DDVKQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPMARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIEISKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            3448..4716
                     /gene="lhgO"
                     /locus_tag="ACHJWA_004033"
     CDS             3448..4716
                     /gene="lhgO"
                     /locus_tag="ACHJWA_004033"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFO4310532.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSELEMERM
                     RALWERTAANGIEREWLNADELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSGLSEHKSGVVIRTRQGSDYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            4739..6187
                     /gene="gabD"
                     /locus_tag="ACHJWA_004034"
     CDS             4739..6187
                     /gene="gabD"
                     /locus_tag="ACHJWA_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO4310533.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPVWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKAHERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            6201..7481
                     /gene="gabT"
                     /locus_tag="ACHJWA_004035"
     CDS             6201..7481
                     /gene="gabT"
                     /locus_tag="ACHJWA_004035"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFO4310534.1"
                     /translation="MSSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAT
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHNKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            7719..9119
                     /gene="gabP"
                     /locus_tag="ACHJWA_004036"
     CDS             7719..9119
                     /gene="gabP"
                     /locus_tag="ACHJWA_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFO4310535.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYADVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLWMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            9140..9802
                     /gene="csiR"
                     /locus_tag="ACHJWA_004037"
     CDS             9140..9802
                     /gene="csiR"
                     /locus_tag="ACHJWA_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFO4310536.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(9803..10252)
                     /gene="kbp"
                     /locus_tag="ACHJWA_004038"
     CDS             complement(9803..10252)
                     /gene="kbp"
                     /locus_tag="ACHJWA_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFO4310537.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(10336..10494)
                     /gene="yqaE"
                     /locus_tag="ACHJWA_004039"
     CDS             complement(10336..10494)
                     /gene="yqaE"
                     /locus_tag="ACHJWA_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFO4310538.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            10677..10976
                     /gene="ygaV"
                     /locus_tag="ACHJWA_004040"
     CDS             10677..10976
                     /gene="ygaV"
                     /locus_tag="ACHJWA_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFO4310539.1"
                     /translation="MTELTQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            10986..11504
                     /gene="ygaP"
                     /locus_tag="ACHJWA_004041"
     CDS             10986..11504
                     /gene="ygaP"
                     /locus_tag="ACHJWA_004041"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFO4310540.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPAKLRRDQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKKAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVILGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQ"
     gene            complement(11557..11961)
                     /gene="stpA"
                     /locus_tag="ACHJWA_004042"
     CDS             complement(11557..11961)
                     /gene="stpA"
                     /locus_tag="ACHJWA_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFO4310541.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         3013 a         2969 c         3123 g         2986 t
ORIGIN      
        1 cttgtggagt ttatcctcag gatttaaaag acagagaaat tatatcaact tttcacgcag
       61 aaaaagtcat taatgatctg ttaaaaaacg attataaaat ttccctttct ccagatacaa
      121 cttaccgaga gctgaataaa gctgcacagc gtagcattac agcgccagac aggataggag
      181 aagggaaaat atgggtttat caacgagata caatggttga aagaggtgat aacagcggtg
      241 ttcatcagta tggtccagct gaacatttca ctcacattat atctgacaaa ccgtccccaa
      301 aagataagta tgttgcatat gctattaaca ttcctgacta tgagctagca gccgatgtat
      361 ataatattaa cgtgacgtca ccttccggac agcaagaaac atttaaaata ttaatcaatc
      421 cagaacatct acggcaaaca cttgagcgta aatcccttac tgctgttcag aaatcacaat
      481 gtgaaatcat cacccccaaa aaacctggcg aagcgattct tcatgctttt aatgccacct
      541 accagcaaat cagggaaaat atgcctgagt ttgcacgttg ccattatggg tatatacaaa
      601 tccctccagt gacaactttc cgcgccgacg gaccagaaac tcccgaagaa gaaaaaggtt
      661 actggtttca cgcttatcaa cccgaagatc tttgtaccat ccataatcca atgggagatt
      721 tgcaggattt tatcgcattg gttaaagatg ctaaaaaatt tggtatcgat atcattcctg
      781 attatacctt taactttatg ggaatcgggg gtagtggtaa aaatgacctg gattatccct
      841 ctgctgatat acgagcgaag atcagtaaag atatagaagg tggtatccct ggctattggc
      901 aaggtcaggt tttgattcca tttactatag atccagtaac aaaagaacgt aaacaaatcc
      961 atccagaaga tatacatctc acagcaaaag acttcgaagc aagtaaagat aatatttcta
     1021 aggatgaatg ggaaaacctc catgcattaa aagaaaagcg tttaaatgga atgcctaaaa
     1081 ccccccccaa aagtgactag gttattatgt tgcaaaatca atacgttcgt gaaatgcgaa
     1141 aatatggcgt acgaggttta cgttacgatg cagcaaaaca ctcaaaacat gaacaaatag
     1201 aaagatcaat aaccccaccg cttaaaaatt ataatgagcg attacacaat actaacttat
     1261 ttaacccaaa atatcatgaa aaagccgtta tgaattacat ggaatatctg gtaacttgcc
     1321 agttggatga agaacaaatg tcatcgctgc tttatgaaag agatgattta agcgccattg
     1381 atttttcatt gctcatgaag acgataaaag ctttttcatt tggtggcgat ctccaaaccc
     1441 ttgcatcaaa accgagttcc acaatctcaa gcatcccatc aaaaagacgg atattgatta
     1501 acattaacca cgattttcct aacaatggca atcttttcaa tgactttcta tttaaccatc
     1561 aacaagatga acaattagca atggcatata tggccgctct cccgttcagc aggcctttag
     1621 tttactggga tggccaagta ttaaaatcaa cgactgaaat taaaaattat gatgggtcga
     1681 cgcgtgtcgg cggtgaggcg tggcttaata aaggttgctc tacctatcag cagctctaca
     1741 atgaattcca cgcattatat atagataaag caggaatatg gagcgcattt gagggtgtat
     1801 ttgcaactaa aaacgttctg gcctttagtc gtggggattc ggtgaacatt aatcactctc
     1861 ctcatgatgg actagttata ataaataaag gaaacgaaga agttgaaggt gcctggccta
     1921 acaaattgca acctggaaaa tataaaaaca tggggagtaa tagcgttaac attattatta
     1981 ataatacccg aaaaattatc ccccctggta aagcatttat gcttagaggc ggaactctaa
     2041 atatcaatat tcctgggcgt agcgctcttc ttttagggaa aacaggagaa ccgctgaact
     2101 atctctattt gtaatttaat gttatatctg ccccgctaaa acggggcaga taatatattt
     2161 agtttactag cggtcatttt gcagtgaagc catttaccgt tttttatcga ccagataatc
     2221 tgttctctga tggtaatccc cccctaacct gttgctttag ttattcattt cctgtctcac
     2281 tttgccttaa taccctacgt taaatgttac caatttgttg ctttttatca caataagaaa
     2341 acaatatggc gcatttgtgc gcatttttca gaaatgtaga tatttttaga ttatggctac
     2401 gaaatgagca tcgccatgtc accctacatc tcataagagg atcgcttctg atgaatgcac
     2461 tgaccgccgt acaaaataac gctgtcgatt cagaccagga ctatagcgga ttcactctca
     2521 tcccgtcggc gcaatccccg cgtctgctgg aactcacctt caccgaacag acgaccaaac
     2581 agtttctcga acaggttgcc gaatggcccg tgcaggcgct ggagtacaaa tcgtttctgc
     2641 gttttcgggt aggtaaaatt cttgacgatc tgtgtgcgaa ccagctgcaa ccattgctgt
     2701 tgaagaccct gctaaaccgt gctgaaggtg cgctgttgat caatgcggtg ggtgtcgatg
     2761 atgtgaagca agcggatgag atggtgaagc tggcgacggc ggtggcgcat ctgattggcc
     2821 gttccaattt cgatgcgatg agcggtcagt attacgcgcg ttttgtagtg aaaaatgtcg
     2881 ataactcaga cagctatctg cgtcagccgc accgcgtaat ggagctgcac aacgacggca
     2941 cctacgtcga agagatcact gactatgtgc tgatgatgaa aatcgacgag cagaacatgc
     3001 agggtggaaa ttcgttgctg ctgcatctcg atgactggga acatctggac cactatttcc
     3061 gccatccaat ggcgcgtcgc ccgatgcgct ttgccgcgcc gccgagcaaa aacgtcagca
     3121 aagatgtttt ccatccagtg ttcgacgttg atcaacaggg tcgcccggtg atgcgctata
     3181 tcgaccagtt cgtccagcca aaagacttcg aagaaggcgt gtggttgagc gagctttcgg
     3241 acgccattga aatcagtaaa ggcattcttt ctgtacccgt tcccgttggc aaattcctgt
     3301 tgattaacaa cctgttctgg ctgcacggtc gcgaccgctt tactccgcac ccggatctgc
     3361 gccgtgaact gatgcgtcag cgtggctatt tcgcttacgc cactcaccac tatcagacgc
     3421 atcagtaagc gcaaaggaat tgagcggatg tatgattttg tgattattgg cggcggcatc
     3481 atcggcatgt cgaccgccat gcaactgatt gatgtctatc cggatgcccg cattgcgttg
     3541 ctggaaaaag agtccggccc ggcctgtcat cagacgggcc acaacagcgg cgtgatccat
     3601 gccggggttt attacacgcc cggtagcctg aaggcgcagt tttgcctggc gggaaaccgc
     3661 gccactaaag ccttttgcga tcaaaacggc attcgctatg acaactgcgg caagatgctg
     3721 gtcgccacct ctgaactcga aatggaacgg atgcgcgcgt tgtgggaacg cacggcggca
     3781 aacggtatcg agcgcgagtg gttaaacgcc gatgaactgc gcgagcgcga accgaatatc
     3841 accgggcttg gcggtatttt tgtgccgtcc agcggcattg tcagctaccg cgaagtcacg
     3901 gcggcgatgg caaaaatttt ccaggccaga ggcggcgaga ttatctataa cgccgaagtc
     3961 agcggcctta gtgagcataa aagcggcgtg gtgatacgta cccgtcaggg cagcgactat
     4021 gaagcatcga cgctgattag ctgttccggg ctgatggctg accggctggt gaaaatgctc
     4081 ggcctcgaac cgggctttat tatctgcccg ttccgcggcg agtatttccg cctggcgccg
     4141 gagcataacc agattgttaa ccatctgatt taccccattc ccgacccggc aatgccgttt
     4201 ttgggcgtgc atctcacccg catgatcgac ggcagcgtca ccgtcgggcc aaacgcggtg
     4261 ctggctttca aacgcgaagg ctatcgcaag cgcgacttct catttagcga cacgctggag
     4321 attttgggct cgtcggggat tcgccgggtg ctgcaaaacc atctacgctc aggactgggc
     4381 gagatgaaaa actcgctgtg caaaagcggc tatctgcggc tggtgcaaaa gtattgtccc
     4441 cggctttcgt taagcgatct ccagccctgg cccgccggtg tgcgggcgca ggcggtatcg
     4501 ccggacggca agctgattga cgattttctg tttgtcacca ccccgcgcac gatccacacc
     4561 tgcaatgcgc cctccccggc agcgacatca gcaattccta ttggtgcgca tattgtcagc
     4621 aaggtacaaa cgctgttggc aagccagagt aaccccggac gcacgctgcg agcggcacgt
     4681 agtgtggatg ccttacacgc cgcgtttaat caataacctt tgaaaacagg atgtagcgat
     4741 gaaactgaac gacagtaact tattccgcca gcaggcgttg attaacgggg aatggctgga
     4801 cgccaacaat ggtgaagtca tcgacgtcac caatccggcg aacggcgaca agctgggtag
     4861 cgtgccgaaa atgggcgctg atgaaactcg cgccgctatc gacgccgcca accgcgccct
     4921 gcccgtctgg cgtgcgctca ccgccaaaga acgcgccaac attctgcgca actggttcaa
     4981 tttgatgatg gagcatcagg acgatttagc gcgcctgatg accctcgaac agggtaaacc
     5041 gctggctgaa gcgaaaggcg aaatcagcta cgccgcctct tttattgagt ggtttgccga
     5101 agaaggcaaa cgcatttatg gcgacaccat tcccggtcat caggccgata agcgcctgat
     5161 tgtgatcaag cagccgattg gcgttacagc ggccatcacg ccgtggaact ttccggcggc
     5221 aatgattacc cgcaaagccg gtccggcgct ggcggcaggc tgcaccatgg tgctgaagcc
     5281 cgccagtcag acgccgttct ctgcgctggc gctggcggag ctggcgatcc gcgcgggcat
     5341 tccggctggc gtgtttaacg tggtcaccgg ttcggcgggc gcggtgggta atgaactgac
     5401 cagcaacccg ctggtacgca aactgtcgtt taccggctcg accgaaattg gccgccagtt
     5461 aatggaacag tgcgcgaaag acatcaaaaa agtgtcgctg gagctgggcg gcaacgcgcc
     5521 gtttatcgtc tttgacgatg ccgatctcga taaagccgtg gaaggcgcac tggcctcgaa
     5581 attccgcaac gccgggcaaa cctgcgtctg cgccaaccgt ttatacgtgc aggacggcgt
     5641 gtatgaccgc tttgccgaaa aattgcagca ggcggtgagc aaactgcata tcggcgacgg
     5701 gctggataaa ggcgtcacca tcgggccgct gatcgatgaa aaagcggtag caaaagtgga
     5761 agagcatatt gccgatgcgc tggaaaaagg cgcgcgcgta gtttgcggcg gtaaagcaca
     5821 tgaacgcggc ggcaacttct tccagcccac cattctggtg gacgtgccgg ccaacgccaa
     5881 agtgtcgaaa gaagagacct tcggccccct cgccccgctg ttccgtttta aagatgaagc
     5941 cgatgtgatc gcgcaagcca atgacaccga gtttggcctt gccgcctatt tctatgcccg
     6001 tgatttaagt cgcgtcttcc gcgtgggcga ggcgctggag tacggcatcg tcggcatcaa
     6061 taccgggatt atttccaatg aagtggcccc gttcggcggc attaaagcct cgggtctggg
     6121 tcgtgaaggt tcgaagtatg gcatcgaaga ttacttagaa atcaaatata tgtgcatcgg
     6181 tctttaactg gagaacgcga atgagcagca ataaagagtt aatgcagcgc cgcagtcagg
     6241 caattcctcg tggcgttggg caaattcacc ccattttcgc tgatcgcgcg gaaaactgcc
     6301 gggtgtggga cgttgaaggc cgtgagtatc ttgatttcgc gggcggcatt gcggtgctca
     6361 ataccgggca cctgcatccg aaagtggttg ccgcggtgga agcgcagttg aaaaaactgt
     6421 cgcacacctg cttccaggtg ctggcttacg agccgtatct ggagctgtgc gagattatga
     6481 atcagaaggt gccgggcgat tttgccaaga aaacgctgct ggttacgacc ggttccgaag
     6541 cggtggaaaa cgcggtgaaa atcgcccgcg ctgccaccaa acgtagcggc accatcgctt
     6601 ttagcggcgc gtatcacggg cgcacgcatt acacgctggc gctgaccggc aaggtgaatc
     6661 cgtactctgc gggcatgggc ctgatgccag ggcacgttta tcgcgcgctt tatccttgcc
     6721 cactgcacgg catcagtgaa gatgatgcta tcgccagtat ccaccgaatt tttaaaaatg
     6781 atgctgcgcc ggaagatatc gccgccatcg tgattgagcc ggttcagggc gaaggcggtt
     6841 tctacgccgc gacgcctgcg tttatgcagc gtttacgcgc gctgtgtgac gagcacggga
     6901 tcatgctgat tgccgatgaa gtgcagagcg gcgcggggcg taccggcacg ctgtttgcga
     6961 tggagcaaat gggcgtggca ccagatctca ccacctttgc gaaatcgatc gcaggcggct
     7021 tcccactggc gggcgtcacc gggcgcgccg aagtgatgga tgccgtcgct ccaggcgggc
     7081 tgggtggcac ctatgccggt aatccgattg cctgcgtggc ggcgctggaa gtgttgaagg
     7141 tgttcgagca ggaaaatctg ctgcagaaag ccaacgatct ggggcagaag ttgaaagatg
     7201 gattgttggc gatcgccgaa aaacaccctg agatcggcga cgtacgcggg ctgggggcga
     7261 tgatcgccat cgagctgttt gaagacggcg atcacaacaa gccggacgcc aaactcaccg
     7321 ccgagatcgt ggctcgcgcc cgcgataaag gcctgattct tctctcctgc ggcccgtatt
     7381 acaacgtgct gcgcatcctt gtaccgctca ccattgaaga cgctcagatc cgtcagggtc
     7441 tggagatcat cagccagtgt tttgctgagg caaagcagta gcgccgctcc tatgccggag
     7501 gcgacgctgc gcgtcttgtc cggcctacgg ggatccaggt cggataaggc gtttacgccg
     7561 catccgacaa tctgtacgtg aacaggaata aatctatgtt ggccgggtaa ggcggagccg
     7621 ctctccggca aaaagaatca ataacaatta tacgcgtgac ccgacgcggg aaatgtcggg
     7681 gcgctctccc aagtgacaca ctttcgagag gattcaggat ggggcaatca tcgcaaccac
     7741 atgagttagg cggcgggctg aagtcacgcc atgtcaccat gttgtctatt gccggtgtta
     7801 tcggcgcaag tctgtttgtc ggttccagcg tcgccatcgc cgaagcgggc ccggcggtat
     7861 tactggccta tctgttcgcc gggctactgg tggttatgat tatgcggatg ttggcggaaa
     7921 tggcggttgc cacgcccgat accggttcgt tttccaccta tgccgataaa gctattggtc
     7981 gttgggcggg ttataccatc ggctggttgt actggtggtt ttgggtactg gttatcccgc
     8041 tggaagccaa catcgccgcc atgatcctgc actcatgggt tccaggcatt cccatctggt
     8101 tattttccct cgtcattacc cttgccttaa ctggcagtaa tttattaagc gttaaaaact
     8161 acggcgaatt tgagttctgg ctggcgctgt gcaaagtcat cgctatcctg gcctttattt
     8221 tccttggtgc agtcgcaatt agcggttttt acccgtatgc cgacgtgagc gggatctcaa
     8281 gattgtggga tagcggcggc tttatgccca acggcttcgg tgcggtatta agcgcgatgt
     8341 tgatcaccat gttctcgttt atgggcgcag aaattgtcac cattgccgcc gcggaatccg
     8401 acacgccgga aaaacatatt gtccgcgcta ccaactcggt tatctggcgt atttctatct
     8461 tctatttgtg ctctattttt gtcgtagtgg cgttaatacc gtggaatatg ccggggctga
     8521 aagccgttgg atcttatcgc tcggttctgg aattgctcaa tataccccat gcgaaattaa
     8581 tcatggactg cgtgatatta ctttccgtaa ccagttgcct gaactcggcg ctgtataccg
     8641 cgtcaaggat gctctactcc ttaagccgtc gcggtgatgc gcccgcggta atgggcaaaa
     8701 tcaaccgcag taaaaccccg tatgtggcgg tgttactctc caccggagcg gcatttttaa
     8761 cggtcgtggt gaactattac gcacctgcga aagtgtttaa attcctgatc gacagctccg
     8821 gtgctatcgc cctgctggtt tatttagtca tcgccgtttc acagttgtgg atgcgtaaaa
     8881 ttctgcgagc agaaggaagc gaaattcgct tgcgcatgtg gctttacccg tggctcacct
     8941 ggctggtaat cggctttatt acctttgtgt tggtggtgat gttattccgc ccggcgcaac
     9001 agttagaagt gatctctacc ggcttattag cgatagggat tatctgtacc gtgccgatta
     9061 tggcgcgctg gaaaaagctg gtattgtggc aaaaaacacc cgttcataat acgcgctgat
     9121 aatgatcagg agtcacacca tgaccattac gtctctggat ggctatcgct ggctgaagaa
     9181 cgatattatt cgcggtaatt ttcaaccgga tgaaaaatta cgaatgagtt tgctgacatc
     9241 gcgttatgca cttggcgttg ggccgttacg ggaagctctt tcgcaactgg tggcggaacg
     9301 gctggtcacg gtggtgaatc aaaaagggta tcgggtggcg tctatgtcag agcaggagct
     9361 gctcgatatt ttcgacgccc gcgccaatat ggaagcgatg ttagtgagtc tggcgattgc
     9421 ccgcggtggc gatgagtggg aggcagacgt tctcgcaaaa gcgcatctgc tgagtaagct
     9481 tgaggcctgt gacgccagcg agaaaatgct tgatgagtgg gatctgcgtc atcaggcgtt
     9541 tcatactgca attgtggcgg gctgtggttc tcactatttg ctgcaaatgc gtgaacggtt
     9601 gtttgatctg gcggcgcgtt atcgatttat ctggctgcgg cgaacggtgc tttcggtgga
     9661 aatgctggag gataaacacg atcagcacca gaccctgact gcggcggtac tggcgcgaga
     9721 taccgcgcgc gccagtgagt taatgcgcca gcatttactg acgccaattc ccattatcca
     9781 gcaggcgatg gctggcaatt aactactctt ccggaatacg caacacttgc cccggataaa
     9841 ttttatccgg gctttttagc atcggtttat tcgcttcgaa gattttattg tacagattag
     9901 cgttaccgta gacctgtttg gaaatggcac tcagagtgtc gccagactta acggtataaa
     9961 actggctggc agtggctggt gtcgccgttt tcacctgatc atcaacactg gcaataccgg
    10021 aaatattccc caccgcaaca aggattttct ccttcgcctc ctgactcagg ccgtcaccag
    10081 tgaccgtcgc tttgccatcg gcaatttgaa tgttcacttt atcggcatca ggtataccgg
    10141 ttttgctcag atgctcctgc accttcttcg cctgatcatc tttatcgtgc tgacctgtaa
    10201 ccgcgtccca gagtttttct ccggcatctt tcacaaaatt gaacagaccc atagctacct
    10261 cattcgttaa cggaaacact cagaaagtgt agtagaggaa cgcggcgcgt taacgccgcg
    10321 gggatgagca caggtttaat cgcgggtttg cacccagaac gcgtgaatca aaccggggat
    10381 atacccaagc agtgtcaaca gaatattaat aatgaacgcc caaccgaacc ctttaccgag
    10441 cagcacgccg agcggcggca gaatgatggt gatgacgatt ctccagaaac ccatatgtac
    10501 tccctataag aaaattactt attgtttaaa aagagatttt atctcttaag cgtagtaatt
    10561 ttagcggagg ctgccagttt cagcttacgc ttttactctc atttacgctg ctgaggctgg
    10621 tatatgttgc atttttagtt tagagtttac ttaatttaga aaatacttaa ataactatga
    10681 ccgaactcac gcaattacag gccagtgctg aacaggcagc ggccttattg aaagcaatga
    10741 gccaccctaa acggttgctg attctgtgca tgcttagcgg ttcccccggc accagcgcgg
    10801 gagagctgac gcgcattacc ggactgagcg cctctgcgac atcacagcat ctcgctcgta
    10861 tgcgggacga agggcttatc aacagccaac gggatgccca acgcattcta tattccatta
    10921 aaaatgaggc ggtaaatgcc attatcgcca ccctgaaaaa tgtctattgt ccgtaaggag
    10981 tcactatggc tttgacaacc atttcgccgc atgatgcaca agaattaatc gcacgcggcg
    11041 caaagttaat cgatattcgt gacgctgatg aatatcttcg tgaacatatt cctgaagcag
    11101 atctggctcc attatccgtg ctggaacagt caggtctccc ggctaaatta cgtcgcgatc
    11161 aaattatatt ccactgccag gcaggtaaac gcaccagtaa taacgccgat aaattagcag
    11221 cgattgccgc ccccgcagaa atctttttac tcgaagatgg gattgatggc tggaaaaaag
    11281 caggattacc agtagcggta aataaatctc aacccttgcc gttaatgcgc caggtgcaga
    11341 tcgctgcggg tggtttaata ttaatcggcg ttatactggg ttataccgta aatagcggtt
    11401 tcttcttatt aagcggcttt gttggtgccg ggttactgtt tgcaggaatt agcggttttt
    11461 gtgggatggc aaggttgtta gataagatgc cgtggaacca atgagcttga gaagcgacgc
    11521 cggacgcgcc ctagcagcga catccggcct cagtaattag atcaggaaat cgtcgagaga
    11581 tttaccttct gccagcgcct gagcgattgg cttcggtgta cggccctgac cggtccaggt
    11641 tttagtttca ccgttaacat cggtgaattt atatttcgcc ggacgcggct ggcgtttttt
    11701 accagcacgt ggtgcagcag cagagctatt acccaataac tcttccgggt taattccgtc
    11761 agctttcatc agctccagcc aggtgctaat tttttcctgg cgctctgcca gttcacgctg
    11821 ctgctgttct tcttcttcac gtctttcttt agtgacaacc ctgaattttt cgagcatttc
    11881 ttcaagaacg tcaatggaga attcgcgagc catcgcacgg agggtgcgaa tgttatttaa
    11941 actttgtaac attacggaca taataaagaa aaccttttaa cgccaaaaca aaaaagtatt
    12001 tcgtcgcgta gtgtaagcga tcacttatta tatttccaca aaatttaatc tatttacagt
    12061 cctgcgcgag attatttcag ctgttatttt c
//
LOCUS       DAZFAB010000124        11892 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZFAB010000124 DAZFAB010000000
VERSION     DAZFAB010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.124"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            67..669
                     /gene="lpfA2"
                     /locus_tag="ACHJWA_004043"
     CDS             67..669
                     /gene="lpfA2"
                     /locus_tag="ACHJWA_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA2"
                     /protein_id="HFO4310542.1"
                     /translation="MKPNMIVGALALTSVFMAGHLQAADGTVHFRGEIIDSTCEVTPE
                     TKDQVVDLGKVNRTAFSGVDDVAAPTAFSIDLTQCPETFKSAAIRFDGNEDAHGNGNL
                     AIGTPLDNSNDAAAGISPSDNSGDYTGAGAVSAAKGVAIRLYNRADNTQVKLYENSAS
                     TPISNGNASMKFMARYIATETTIDPGTANADSQFTVEYIK"
     gene            830..1156
                     /locus_tag="ACHJWA_004044"
     CDS             830..1156
                     /locus_tag="ACHJWA_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO4310543.1"
                     /translation="MATAGIVVGGTRVIFYGDKPDTTISIFNKETNLPYLLQTWVDPF
                     NKEDKSKPPFSVIPPVSRIEPQQEKILRILKTAGELPKDRESVFWLNVKNIPPSIDKK
                     IPMFLR"
     gene            1309..1458
                     /locus_tag="ACHJWA_004045"
     CDS             1309..1458
                     /locus_tag="ACHJWA_004045"
                     /inference="COORDINATES: protein motif:HMM:NF014773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4310544.1"
                     /translation="MDDKDVSLNMIRPFEILTLPIPAGVAGKSLVWRFINDYGAISQP
                     LKKNL"
     gene            1480..4014
                     /locus_tag="ACHJWA_004046"
     CDS             1480..4014
                     /locus_tag="ACHJWA_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO4310545.1"
                     /translation="MASSHLFITLASGICLLCSISAFARDSLFNPRLLELDHPADNID
                     IHQFNRSNTLPAGTYKVDVMINGMLFERQEVKFAQDNPDAELHPCYVAIKNVLATYGI
                     KVDAIKSLANVDDKTCVNPVPLIDGATWLLDASKLALNITIPQIYLNNAVNGYISPSR
                     WDQGINAMMMNYDFSASHTIRSNYDDDDDSYYLNLRNGINLGAWRFRNYSTLNSYDGN
                     VDYHSVSNYIQRDIMALRSQIMIGDTWTASDVFDSTQVRGVRLYTDDDMLPSSQNGFA
                     PVVHGIAKTNATVIIKQNGYVIYQSAVPQGAFALTDLNTTSSGGDLDVTIKEEDGSEQ
                     HFIQPFTSLAILKREGQTDVDLSIGEVRDESGFTPEVLQLQAMHGFPLGITLYGGTQL
                     ANDYASAALGIGKDMGALGAISFDVTHARSQFDYDDNESGQSYRFLYSKRFEDTNTTF
                     RLVGYRYSMEGFYTLNEWVSRQDNDSDFWVTGNRRSRFEGTWTQSFTPGWGNIYLTFS
                     RQEYWQTDEVERLLQFGYNNNWRNISWNVSWNYTDSIKRSLGNHHDDNNDDFGKEQIF
                     MFSMSIPLSCWMEDSYVNYSLTQNNHHESTMQVGLNGTMLEGRNLSYNVQESWMHSPD
                     DSYSGNAGMTYDGTYGSVNGSYSWSRDSQHFDYGARGGVLVHSDGVTFSQELGETVAL
                     VKAPGAEGLSIENATGISTDWRGYTVKTQLSPYDENRVALNSDYFSKANIELENTVIN
                     LVPTRGAVVKAEFVTHVGYRVLFNVRQVNGKPIMFGAMATASLETGTVTGIVGDNGEL
                     YLSGMPEKGEFLLSWGQAADEKCKAAYHITHKPDDTSLVQMDAICR"
     gene            4026..5096
                     /locus_tag="ACHJWA_004047"
     CDS             4026..5096
                     /locus_tag="ACHJWA_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4310546.1"
                     /translation="MKKILSGLILLLCCPYGFAANGDGATHMSNLSFGPLTVAAANNH
                     SGYNIFEALSNTTGTYPVRCHCDDTHGGPGQQTAFFPIFYTGDAAPGLVLERTLNGLN
                     YYALNDYLSVGVTIFIINNQYAAIPFEHLSNQSTSPQHTCGAGNNGSTVNLDSGRSAK
                     LSFYVRHSITGTVTIPTTEVAWLYAGMSDHFPKTTPVSKVTIRGQLTAPQNCELTPNQ
                     SIDVDFQKINSAEFSSTAGSIIAERKIKTEVTVSCTGMEDVRSTEVVSASMIAANRSA
                     DATMIVTSNPDVGIKIFDKNDRPVNVDGGNLPADMGAISRLGKTDGSVTFYSAPASLT
                     GAKPAPDNGFTATATLVIEFTN"
     gene            5124..6206
                     /locus_tag="ACHJWA_004048"
     CDS             5124..6206
                     /locus_tag="ACHJWA_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4310547.1"
                     /translation="MKKWHYIFCIILFHLGLPCGYAANDGTCATRGGTHTLSLNFPLT
                     TVSAANNVPGNTLIDIANATSSENYSVLCNCDSKHSNGAYHEIYYTADPAPGMVYSTT
                     ASGLAFYYLNEYVDVGTKISVLNAGYTAVPFEHVSNQATTTDHTCQGNKTTAVGVSLK
                     TGADAKISFRIKRSINGTVVIPITDIALLYANISSTTTRGEAIAKVRISGSLTAPQSC
                     QINAGQVIYFDFDTIPASEFSSTAGQAITSRKITKTVSIECTGMGYERTQKVDASFTG
                     TNRSSDDTMVATDNADVGIKIYNKSNAEVSVNNGKLPADMGNTTIFGRKNGSVTFSAA
                     PASFTGARPQPGVFNATATLTIEFVN"
     gene            6418..7458
                     /gene="pstS"
                     /locus_tag="ACHJWA_004049"
     CDS             6418..7458
                     /gene="pstS"
                     /locus_tag="ACHJWA_004049"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFO4310548.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVLPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            7545..8504
                     /gene="pstC"
                     /locus_tag="ACHJWA_004050"
     CDS             7545..8504
                     /gene="pstC"
                     /locus_tag="ACHJWA_004050"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFO4310549.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            8504..9394
                     /gene="pstA"
                     /locus_tag="ACHJWA_004051"
     CDS             8504..9394
                     /gene="pstA"
                     /locus_tag="ACHJWA_004051"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFO4310550.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            9577..10350
                     /gene="pstB"
                     /locus_tag="ACHJWA_004052"
     CDS             9577..10350
                     /gene="pstB"
                     /locus_tag="ACHJWA_004052"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFO4310551.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            10365..11090
                     /gene="phoU"
                     /locus_tag="ACHJWA_004053"
     CDS             10365..11090
                     /gene="phoU"
                     /locus_tag="ACHJWA_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFO4310552.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDPKE"
     gene            11515..11649
                     /locus_tag="ACHJWA_004054"
     CDS             11515..11649
                     /locus_tag="ACHJWA_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_307391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310553.1"
                     /translation="MAISSYSTVTDKFANLTKMPDENNGLQKNQKNFVCLLVMRRSIQ
                     "
     gene            11616..>11892
                     /locus_tag="ACHJWA_004055"
     CDS             11616..>11892
                     /locus_tag="ACHJWA_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001310363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III effector protein"
                     /protein_id="HFO4310554.1"
                     /translation="MSVGNEEKYSITHKDITNLIAGDRQSFLKLTLWEFILDLFPMYH
                     IKEAKETIVEFVTKTEDKVKRFERIKSLAIPEEKWRFSTTTEFTVDEN"
BASE COUNT         3138 a         2734 c         2931 g         3089 t
ORIGIN      
        1 aggtttatcc aaatttaata aatggataac acacaggaat aatatcaaat taatggagtt
       61 atatttatga aaccaaatat gattgtagga gcattagcgt taacttctgt gtttatggca
      121 ggtcacctac aggcggctga tggaacagtc catttccgtg gtgaaattat tgacagtact
      181 tgcgaagtca ctcctgaaac taaagatcag gtcgttgatt taggcaaagt aaaccgtaca
      241 gcctttagtg gcgtcgatga tgtggctgcc ccgacggctt tttctatcga tctgactcaa
      301 tgcccggaaa cctttaagtc cgccgcaatt cgtttcgatg gtaatgaaga tgctcatggt
      361 aatggcaacc tggcaattgg taccccgctg gataactcta acgatgctgc cgctggtatt
      421 agcccgagtg ataacagtgg ggattatact ggtgcgggtg ccgttagtgc agcgaaaggc
      481 gtagctattc gtttatataa ccgtgcagat aacactcagg tcaagttata tgaaaattct
      541 gcatcaactc cgatttctaa tggtaatgca tccatgaagt tcatggctcg ttatattgct
      601 acggaaacga ctattgaccc tggtacagct aacgccgact cgcagtttac agttgaatat
      661 ataaaataaa tcattcttaa cataatacct taaataaaat ggcgctcgcc attatggttc
      721 tcttttactt aaatgaaggg cgtcattatt atatttttta tgagatacat ttatgcctat
      781 gtatttactt agattgtttt ttgctgttac tttgattacg cagatgtcga tggctacggc
      841 tggtattgtt gttgggggaa ctcgcgttat tttttatggt gacaaacctg ataccacaat
      901 ctctattttt aataaagaaa cgaaccttcc ttatctattg cagacatggg ttgatccatt
      961 taataaagag gataaaagca aaccgccatt ttctgtgata cctcccgttt ctcgcattga
     1021 accacagcaa gagaaaatat tacgcatttt aaaaacggct ggcgagttac caaaagatcg
     1081 ggagtccgta ttctggttga acgtgaaaaa catccctcct tcaattgata aaaaaatacc
     1141 aatgttcttg agatagccat aaaaacaaga attaagttat tctggcgacc agccgcggta
     1201 aagataatcc ctgagcaggc tattaaaaat gttaaatggt cgcggcaggg taataatctc
     1261 atcgtcgaaa atccaaatcc gatccatatt aatgtaatga gtgtgacggt ggatgataaa
     1321 gacgtgtcgc taaatatgat ccgccctttt gaaatattga cattaccaat tccggctggc
     1381 gtggcaggaa aatcgctcgt ttggcgcttt attaatgatt acggtgcaat tagtcaacca
     1441 ctaaagaaaa acctttaagc gccggataag gatatatcga tggcctcttc gcatcttttc
     1501 attacgcttg catcgggcat atgtctgctc tgttccatat ctgcttttgc ccgggatagc
     1561 ttgttcaacc ccagattact ggaactggat catcctgcgg ataatattga tattcaccag
     1621 ttcaaccgtt cgaatacctt acctgcggga acatacaaag ttgatgtgat gatcaacggc
     1681 atgctcttcg aacgccagga agttaaattc gcccaggata accctgatgc tgaactccac
     1741 ccatgctacg tggcgataaa aaacgtgctg gcgacctatg gtataaaagt tgatgcgata
     1801 aaatctctgg cgaatgttga tgacaaaaca tgcgtaaatc cagttccgct gatcgacggg
     1861 gctacctggt tactggacgc cagtaaactt gcattgaata ttactattcc gcaaatttat
     1921 ctcaacaatg cagttaatgg ttatatcagc ccttcccgtt gggatcaggg gatcaatgcc
     1981 atgatgatga attatgattt ttcggcatcg cataccatcc ggtcaaatta tgacgacgac
     2041 gatgacagtt attatctgaa tttgcgtaat ggtattaatt taggcgcatg gcgttttcgt
     2101 aattacagca ccctgaattc ttatgacggt aatgtggact accattccgt cagtaattac
     2161 attcagcgcg acatcatggc attacgtagc cagattatga ttggcgatac ctggacggca
     2221 agcgatgtat ttgatagtac acaggtgcgt ggcgtgcggc tgtataccga tgacgatatg
     2281 ttgccctcca gccagaacgg ctttgcgcca gtggtacatg ggattgcgaa aactaacgcc
     2341 acggtgatca tcaaacaaaa cggctacgtt atttatcaat cagccgtacc acagggcgca
     2401 tttgccctca ccgacttaaa cacgaccagt agcggcggcg atctcgatgt cactatcaaa
     2461 gaagaagatg gcagcgagca gcactttatt cagccattta cttcactggc cattctcaag
     2521 cgtgaaggtc agaccgatgt agaccttagc attggagaag tgcgcgacga aagcggcttt
     2581 acgcctgagg tcttgcagtt acaagcaatg cacggtttcc ctttgggaat aactttgtat
     2641 ggcggaacac aattggcaaa tgattacgct tctgccgcgc tgggtattgg taaagatatg
     2701 ggggcgctgg gcgcgatttc ttttgacgtg actcatgccc gctcgcagtt tgactacgac
     2761 gataatgaga gtggtcaatc gtatcgtttt ctctattcca aacgttttga agacaccaat
     2821 accacctttc gtctggtggg ttatcgctac tctatggagg ggttctacac cctcaatgaa
     2881 tgggtgtcgc gacaggataa tgattctgat ttctgggtaa cgggcaaccg tcgcagccgc
     2941 ttcgaaggca cctggacgca atctttcacg ccaggctggg gcaatattta tttaacattc
     3001 agtcgacagg aatactggca gaccgatgag gtcgaacgtt tattacagtt cggctataac
     3061 aacaactggc gaaacatctc ctggaacgtt tcctggaact atacggactc gatcaagcgc
     3121 tcattgggca accatcatga tgataacaat gatgatttcg gcaaagaaca gattttcatg
     3181 ttctcaatgt cgataccgct atcgtgctgg atggaagaca gctacgtcaa ctattcgtta
     3241 acgcaaaaca accaccatga aagcacgatg caggtcggtc tgaacggaac gatgctggaa
     3301 gggcgtaacc tgtcttataa cgtacaggaa tcgtggatgc actctcctga tgactcctac
     3361 agcggcaatg ccggaatgac ctatgacggg acttatggct cggtcaatgg tagctattcc
     3421 tggagccgtg actcccaaca ttttgattat ggcgccagag gcggcgtgct ggtgcatagt
     3481 gacggagtga ccttctcgca ggaactgggc gaaacggtgg cattggtcaa agcgccgggc
     3541 gcagaaggcc tgtccattga aaacgccacc gggatttcta ccgactggcg tggttatacc
     3601 gtaaaaacgc agcttagccc gtatgacgaa aaccgcgtgg cattgaacag cgactatttc
     3661 tccaaagcca atattgaact ggaaaacacc gtcatcaacc tggtaccaac gcgcggtgcg
     3721 gtggtgaaag ccgaatttgt cacccatgtc ggttatcgcg tgctatttaa cgtccgccag
     3781 gtcaacggta aaccaataat gtttggcgcg atggcaaccg cctctctcga aacgggcaca
     3841 gtcaccggga ttgtcggtga taacggcgaa ctgtatctct ccgggatgcc tgaaaaaggc
     3901 gagtttttat tgagttgggg acaagctgcg gatgaaaaat gtaaggcggc ctatcacatc
     3961 acccataaac ctgatgatac cagcctggtt caaatggatg cgatttgtcg ctaaaggaag
     4021 catgaatgaa aaaaatatta tcagggttga ttctgctgct ttgctgtcct tatggtttcg
     4081 ccgctaatgg tgatggcgca acgcacatgt caaatttatc atttggtccg ctgacggtgg
     4141 cagcggcgaa taaccactcc ggatacaata ttttcgaggc actgagcaac acgactggaa
     4201 catacccggt gcgctgtcac tgtgatgaca cgcatggcgg accgggccaa caaacagcat
     4261 tttttcctat cttctacacg ggagatgccg caccggggct tgtgcttgag cgcactctta
     4321 atgggttaaa ttactatgct ctgaatgatt atttatcggt cggcgtgacg atttttatta
     4381 ttaataacca atatgcggcc attccttttg aacacttatc caaccaatcc acctcaccgc
     4441 aacatacctg tggagcaggt aataatggga gcactgtaaa tctggattca gggcgctcgg
     4501 caaaattatc tttctatgtt cggcattcta ttactggcac ggtgacaata cccacaacgg
     4561 aagtcgcctg gttgtacgcg ggcatgtccg atcattttcc caaaacgacc cccgtttcta
     4621 aagtgacaat tcgcggacaa ctaacggccc cgcagaactg tgagttaacg ccaaatcaga
     4681 gcatcgatgt cgattttcaa aaaattaata gcgctgagtt ctcctcaacg gcgggttcaa
     4741 ttattgcaga aagaaagatt aaaaccgaag tcacagtatc ctgtaccggg atggaagacg
     4801 taaggtccac ggaggtggtg agtgcgtcga tgattgcggc aaacagaagt gccgatgcca
     4861 ccatgatcgt gacgagtaat ccggatgtgg gaattaagat ttttgataag aacgaccgtc
     4921 cagtgaatgt ggatgggggc aacttacccg ctgatatggg tgctattagt cgattaggaa
     4981 aaaccgatgg tagcgtaacg ttttattcag cgcccgccag tctgacgggc gcaaaaccag
     5041 cgcctgataa tggatttacc gctacagcca cgctggttat tgaatttact aactaatctt
     5101 gtgttagtaa aaggaataat ttcatgaaaa aatggcatta tatattttgc ataattctct
     5161 ttcatttagg gttaccgtgc gggtatgcgg caaatgatgg cacgtgtgca acaagaggcg
     5221 gcacacatac attaagcctt aattttcctc tgacaacggt cagtgcagca aacaatgtgc
     5281 ctggaaatac attaatagat attgctaatg caacatcttc tgaaaattat agcgttctgt
     5341 gtaactgtga ttcaaaacat agcaatggcg cttatcacga aatatattat accgcagacc
     5401 ctgctcccgg tatggtttat agcaccaccg caagtggtct tgctttttac tatcttaacg
     5461 aatatgtcga tgtgggaaca aaaatatctg tgctaaatgc ggggtatacg gcagttcctt
     5521 ttgaacatgt ttccaaccag gcaactacaa cagatcacac ttgtcaggga aacaaaacta
     5581 cagcggttgg cgtgagcctg aaaactggag cagatgcgaa gatttcattt cgtattaaac
     5641 gttcaataaa tggaacggta gtaataccta tcaccgatat tgcattgctg tatgccaaca
     5701 tatccagcac cacgacccgt ggtgaggcga ttgcaaaagt tcgaatttca ggcagtttga
     5761 ccgcaccaca gtcttgtcag ataaatgcag gacaggtgat ttattttgat tttgatacta
     5821 ttcctgcgtc cgaattttca tctaccgccg ggcaagccat tacttcacga aaaatcacta
     5881 aaacagtgag tattgagtgt acggggatgg ggtatgagcg tacgcagaaa gtcgatgctt
     5941 cttttacggg gacgaaccga agcagtgacg atacgatggt ggcgacagac aatgctgatg
     6001 tcgggatcaa aatttacaat aaatcgaatg ctgaagttag cgtcaacaac ggcaagttac
     6061 ccgcagacat gggcaacacg accatttttg gtcgtaaaaa tggttcggta actttttcgg
     6121 cagcacctgc cagctttacc ggtgcccggc ctcagcccgg cgtttttaac gctaccgcga
     6181 ccttaaccat tgaatttgta aactaacctt ttgcggctgt tttatgacag ccgttcatat
     6241 ttcctctcct ttcatgaaaa aatgctgata tttttcccct tcaatatcaa taagtaataa
     6301 ctttcctttg ggtgtcatca aactgtcaca ttccttacat ataactgtca cctgtttgtc
     6361 ctattttgct tctcgtagcc aacaaacaat gctttatgaa tcctcccagg agacattatg
     6421 aaagttatgc gtaccaccgt cgcaactgtt gtcgccgcga ccttatcgat gagtgctttc
     6481 tctgtgtttg cagaagcaag cctgacaggt gcaggtgcaa ccttccctgc gccggtgtat
     6541 gccaaatggg ctgacactta ccagaaagaa accggtaata aagttaatta ccagggtatc
     6601 ggttcttccg gtggcgtaaa acagattatc gccaataccg ttgattttgg tgcttctgac
     6661 gcgccgctgt ctgacgaaaa actggctcag gaaggtctgt tccagttccc gaccgtgatt
     6721 ggcggcgtgg tgctggcggt taacattcca ggtctgaagt ctggtgagct ggtgctggat
     6781 ggtaaaaccc tcggcgatat ctacctgggc aaaatcaaga agtgggatga tgaagccatc
     6841 gccaaattga atccgggtct gaaactgcct tcacagaaca ttgctgtagt gcgccgcgca
     6901 gatggctccg ggacttcctt cgtcttcacc agctacctgg cgaaagtgaa tgaagagtgg
     6961 aaaaacaacg ttggtactgg ctctaccgta aaatggccga tcggtctggg tggtaaaggt
     7021 aacgacggta tcgctgcgtt cgttcagcgt ctgccgggtg caattggtta cgttgaatat
     7081 gcttatgcga agcaaaacaa cctggcgtac accaaactga tctccgctga tggtaaacct
     7141 gttttgccga ccgaagaaaa cttcgctaat gcagcaaaag gtgcagactg gagcaaaacc
     7201 ttcgctcagg atctgaccaa ccagaaaggc gaagatgcgt ggcctattac ctctaccacg
     7261 ttcattctga tccacaaaga tcagaagaaa ccagaacaag gcacagaagt gctgaaattc
     7321 ttcgactggg cgtacaaaac cggggctaaa caggcgaacg acctggatta cgccagcctg
     7381 ccggatagcg tagttgaaca ggttcgcgct gcgtggaaga ccaatattaa agacagtagc
     7441 ggcaagccgc tgtactaata aaactccagg ccggataagg cattaaacgg cttatccggc
     7501 attacaaaat gactttgtaa acgcgtttaa ctgaagagta acttatggct gcaaccaagc
     7561 ctgcttttaa cccaccgggt aaaaagggcg acataatttt cagcgtgctg gtaaaactgg
     7621 cggcgctgat tgtgctattg atgttgggtg gcattattgt ctctctgatc atctcctcat
     7681 ggccgagcat tcagaaattt ggtctggctt tcctgtggac caaagagtgg gatgcgccga
     7741 acgatatcta cggggcgctg gtgccgatct acggtacgtt ggtgacttcg tttatcgcgc
     7801 tgctgatcgc cgtcccggtg agtttcggta tcgccctgtt cctgactgag cttgcgcctg
     7861 gctggctgaa acgcccgctg ggtatcgcca ttgagctgct ggcagccatt ccaagtatcg
     7921 tttacggcat gtggggcctg tttatctttg cgccgctgtt cgccgtttac ttccaggagc
     7981 cggtcggcaa tatcatgtcg aatatcccga ttgtcggcgc gctgttctct ggcccggcat
     8041 ttggtatcgg tatcctcgcg gcaggcgtga tcctcgccat catgattatt ccgtacattg
     8101 cggcggtaat gcgcgatgtg ttcgaacaaa ccccggtgat gatgaaagag tcggcctacg
     8161 gtattggctg caccacctgg gaggttatct ggcgtatcgt tcttccgttc accaaaaatg
     8221 gtgttatcgg cggcattatg ctggggctgg gccgcgcgct tggtgaaacc atggcggtga
     8281 cctttattat cggtaacacc taccagctcg acagcgcctc gctgtacatg ccgggcaaca
     8341 gtatcacctc tgcgctggcg aacgaatttg ccgaagcgga atccggtctg cacgttgccg
     8401 cactgatgga actgggcctg atcctgtttg tgattacctt catcgtcctc gccgcatcga
     8461 agtttatgat tatgcgcctg gctaagaatg agggggctcg ctaatggcta tggttgaaat
     8521 gcaaaccact gcggcgctgg cggaatcccg ccgcaaaatg caggcgcgtc gccgcctcaa
     8581 aaaccgtatt gcactgacgc tctcaatggc gacgatggcc ttcggtctgt tctggctgat
     8641 ctggatttta atgtccacca tcacccgcgg tatcgacggt atgtcgctgg cgctgttcac
     8701 tgaaatgacg ccgccgccca atacggaagg tggtggtctg gcgaacgctc tggcaggtag
     8761 cgggctgtta attttgtggg ccacggtatt cggtacgccg ctgggcatta tggcggggat
     8821 ttatctggct gaatatggtc gtaaatcctg gctggcagaa gtgattcgct tcattaacga
     8881 cattctgctc tctgcgccgt cgattgtggt tggtctgttt gtttacacca tcgtggtggc
     8941 gcagatggag cacttctccg gttgggcggg tgtgattgcg ctggcgttgt tgcaggtgcc
     9001 gattgttatc cgcaccaccg agaacatgct gaaactggtg ccggacagcc tgcgtgaagc
     9061 ggcttatgcg ctgggtacac cgaagtggaa gatgatctcc gcgattacgc tgaaagcgtc
     9121 ggtgtccggg atcatgaccg gtatcctgct ggcgattgcc cgtattgctg gtgaaaccgc
     9181 gccgctgctg tttaccgcgc tctccaacca gttctggagc acggacatga tgcagccgat
     9241 cgccaacctg ccggtgacga tctttaagtt tgcgatgagc ccgtttgcgg aatggcagca
     9301 actggcctgg gccggggtat tgatcattac cctgtgcgta ctgctgctga acattctggc
     9361 gcgcgttgtt ttcgcgaaga ataaacacgg ttgatattgc tgacacggtt ttcccctcac
     9421 cctaaccctc tccccagagg ggcgagggga ccgaccgagc gcctttttga ctctgtacac
     9481 ggttaacact ttgccggatg cggcgtgaac gcctgatccg gcctacggta agcctgattc
     9541 gcgaagtgca tcaggcacga tgaggaaaag attgcaatga gtatggttga aactgccccg
     9601 agtaaaattc aggttcgtaa tttgaacttc tactacggca aattccatgc cctgaaaaac
     9661 atcaacctgg atatcgccaa aaaccaggta acggcgttta tcgggccgtc cggctgcggt
     9721 aaatcgacgc tgctgcgtac cttcaacaaa atgtttgaac tgtacccgga acagcgtgcg
     9781 gaaggtgaaa ttctgctcga tggcgacaac atcctgacca actctcagga tatcgccctg
     9841 ctgcgtgcga aagtgggcat ggtgttccag aaaccgacgc cgttcccgat gtccatctac
     9901 gacaacatcg cctttggcgt tcgtctgttt gagaagctct ctcgtgccga catggacgag
     9961 cgcgtgcagt gggcattgac caaagccgca ttgtggaacg aaaccaaaga taaattgcat
    10021 cagagcggtt actctctctc tggtggtcag caacagcgtc tgtgcattgc gcgtggtatc
    10081 gccattcgcc cggaagtgct gctgctcgac gaaccatgtt cggcgctcga ccccatctcc
    10141 accgggcgta ttgaagagct gatcaccgaa ctgaagcagg attacaccgt ggtgatcgtc
    10201 acccacaaca tgcagcaggc tgcgcgttgt tccgaccaca cggcgtttat gtacctgggc
    10261 gaattgattg agttcagcaa cacggacgat ctgttcacca agccagcgaa gaaacaaaca
    10321 gaagactaca tcaccggtcg ttacggttga ttcaggagtg cgttatggac agtctcaatc
    10381 ttaataaaca tatttccggc cagttcaacg ccgaactgga aagtatccgc acgcaggtga
    10441 tgaccatggg cggcatggtg gagcagcagc tttctgatgc aatcaccgcg atgcataacc
    10501 aggacagcga tctggcgaag cgcgtgatcg aaggcgacaa gaacgtcaac atgatggaag
    10561 tggcgatcga tgaagcctgc gtgcgcatta ttgccaaacg ccagccgacg gcgagcgacc
    10621 tgcgattggt tatggtgatc agtaaaacca ttgccgaact ggagcgtatt ggcgacgtgg
    10681 cggacaaaat ctgccgtact gcgctggaga aattctccca gcagcatcag ccgttgctgg
    10741 taagtttgga gtcgctgggc cgtcatacta tccagatgct gcacgacgtg ctggacgcgt
    10801 tcgcgcggat ggacattgac gaagcggtac gtatttatcg tgaagataaa aaagtcgatc
    10861 aggagtacga aggtattgtt cgtcaactga tgacctacat gatggaagat tcgcgcacca
    10921 ttccgagtgt acttaccgcg ctgttctgtg cgcgttctat cgaacgtatt ggcgaccgct
    10981 gccagaatat ttgtgagttt atcttctact acgtgaaggg tcaggatttc cgtcatgtag
    11041 gcggtgatga gctggataaa ctgctggcgg gaaaagaccc caaagagtag tgctaactgt
    11101 aggccagata aagctttttg ccgcatctgg caatcagccc ggtcatacat agttaccggg
    11161 ctgatacact ttctcactga aatattgcca cacaaactca cctcgaaact ctccccagta
    11221 cattgaatat acattatttc atttgaatta ttttactttt aatgtagtgc ctcgctataa
    11281 aaacatattt gttattcttt tcatttatat cgctacctgc acagagaatg gaaaagaaaa
    11341 caaaaaacag ggtaaaagcc ctatatcgag acataaaaaa tgagcgattt tttacagtat
    11401 tcataggtgg tttagtcata atcgtagcaa accaacaccc aggtatgctt tttaagacaa
    11461 atcggcaatg tattatgctc gggcgaataa atattgtgtt caggagaaat gattatggct
    11521 atatcatcat atagtacagt tacagataaa tttgctaatc tgactaaaat gccagatgaa
    11581 aataatggat tgcaaaaaaa tcagaaaaat tttgtttgtc tgttggtaat gaggagaagt
    11641 attcaataac ccataaagat ataacaaacc ttatagctgg tgatagacaa tcttttctta
    11701 aactaactct ttgggaattt atactcgatc tctttcccat gtatcatatt aaggaggcta
    11761 aagaaacaat tgtagaattt gtaacgaaaa ctgaggacaa agttaagaga tttgagagaa
    11821 ttaaatctct cgctattcca gaagaaaaat ggcgctttag tacgacgaca gaatttacag
    11881 tagatgagaa ta
//
LOCUS       DAZFAB010000125        11887 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZFAB010000125 DAZFAB010000000
VERSION     DAZFAB010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11887
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.125"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            151..3519
                     /locus_tag="ACHJWA_004056"
                     /pseudo
     CDS             151..3519
                     /locus_tag="ACHJWA_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ECSE_1600 family autotransporter"
     gene            3522..4448
                     /gene="hipA"
                     /locus_tag="ACHJWA_004057"
                     /pseudo
     CDS             3522..4448
                     /gene="hipA"
                     /locus_tag="ACHJWA_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
     gene            4719..4988
                     /locus_tag="ACHJWA_004058"
                     /pseudo
     CDS             4719..4988
                     /locus_tag="ACHJWA_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            5422..5985
                     /gene="fimA"
                     /locus_tag="ACHJWA_004059"
     CDS             5422..5985
                     /gene="fimA"
                     /locus_tag="ACHJWA_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO4310555.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
     gene            6346..7056
                     /locus_tag="ACHJWA_004060"
     CDS             6346..7056
                     /locus_tag="ACHJWA_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO4310556.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSNLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDIPHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            7098..9749
                     /locus_tag="ACHJWA_004061"
     CDS             7098..9749
                     /locus_tag="ACHJWA_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO4310557.1"
                     /translation="MTAFRAAFKAYRMHQVLLLPRFARLTIALSLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMTSRDITFITDDNNADLIP
                     CLSTDLLVSLGIKKSALLDNKEHSAEKHVPDNSACTPLQDRLVDASTEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYNSGSSNSSDSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSAANGGDLQVTIKEADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSSPRFIQGSLMHGLEGNWTPYGGMQIAEDYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADGTRHSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDSNEQTQFIDYFNLFYSKRGQEQISISQQLGNYGATFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTHGGNTSSGTSGYSTLNYRGAY
                     GNTNVGYSRSGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVVTVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGKLQVSWGNDK
                     NSNCIVDYKLPEVSPGTLLNQQTAICR"
     gene            9763..10293
                     /gene="ydeS"
                     /locus_tag="ACHJWA_004062"
     CDS             9763..10293
                     /gene="ydeS"
                     /locus_tag="ACHJWA_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4310558.1"
                     /translation="MKYNNIIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDGNMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            10306..10809
                     /gene="ydeR"
                     /locus_tag="ACHJWA_004063"
     CDS             10306..10809
                     /gene="ydeR"
                     /locus_tag="ACHJWA_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4310559.1"
                     /translation="MKRLHKRFLLATFCALLTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPAETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDATLKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            10869..11783
                     /gene="ydeQ"
                     /locus_tag="ACHJWA_004064"
     CDS             10869..11783
                     /gene="ydeQ"
                     /locus_tag="ACHJWA_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4310560.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVNLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGTTTDSARQVFANTAPDATKASGVGVSLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
BASE COUNT         3318 a         2505 c         2881 g         3183 t
ORIGIN      
        1 ttcgaaagta ttagcaagtt agccaaattg acactttccc acctccaact aacactcaat
       61 acagacctct gacaatttaa taaatttcat ttttaatgtc tgacgactca tctctttcgt
      121 ggctgtattg tttgtatttg gagcgtttat atgaatagaa tctatcgcgt gatatggaat
      181 tgcactctac aggtatttca ggcctgctcg gaattaactc gcagggtagg taaaacatca
      241 acggttaatt tgcgaaagtc ctctggactg acaacgaaat tcagtagatt gacgctgggt
      301 gttttgctgg cactaagcgg ttcagtgtct ggtgcgagtc tggaagttga taatggtcag
      361 attaccaata ttgatactga tgttgcttat gatgcctacc tggttggctg gtatggcact
      421 ggagtgctta atattttggc tggcggtaat gcctccttaa ccactattac taccagcgtc
      481 attggcggta atgaggattc ggagggtacc gttaatgttt tgggtggcac ctggcgattg
      541 tatgatagcg gaaataatgc aaggccttta aatgtgggtc aatccggaac ggggacgctg
      601 aatattaaac agaagggtca cgtcgatgga ggctatttaa gattaggtac tcaagctgca
      661 ggcgtcggga cggttaatgt tgagggagag gactcggttt tgacgaccga attattcgaa
      721 atagggagtt acggtacggg ctcattaaat attacggata agggttacgt cacgagttca
      781 atagtcgcca ttttaggata tcaagcgaac agtaatggta aggttgtcgt tgaaaagggt
      841 ggcgagtggc taataaaaaa taatgattcc tcaattgaat ttcaaattgg taatcaagga
      901 actggggagg cgactattcg cgagggtggg ctgattacgg ctgaaaatac gattatcggt
      961 ggcaatgcca ccggtgtcgg aaccctgaat gtgcaggatc aagactctgt catcacggta
     1021 cgcagactct ataatggata tttcggtaat ggcgcagtca atatttccaa taatggacta
     1081 attaataaca aagaatattc attggtgggc gttcaggacg gttcccacgg tgtcgtcaac
     1141 gtgaccgata aagggcattg gaatttcctc ggaacgggcg aagctttccg ctatatctat
     1201 atcggtgatg ctggcgacgg tgaacttaat gtctcgcgtg aaggtaaagt agattcggga
     1261 attatcactg cggggatgaa agaaacaggc acaggcaacc ttactgttaa ggataagaac
     1321 tccgttatca ctaatctcgg aactaatctt ggttatgacg gccacggcga aatgaatatc
     1381 agtaatgagg ggcttgttgt cagcaacgga ggaagctctc tcggttatgg agaaaccggc
     1441 gtcgggaagg ttagcatcac cacggggggg atatgggagg tcaataagaa tgtctatacc
     1501 accattggtg ttgcgggcgt cggaaacctc aatattagcg atggcggtaa gttcgtatcg
     1561 caaaatatta cttttttggg cgataaagca agcggtatcg gcacactgaa cctgatggat
     1621 gcgacatcgt cgttcgatac tgtgggtatc aatgtcggta attttggtag cggtatcgta
     1681 aatgtcagta atggtgccac ccttaattca acgggctatg gatttatcgg aggaaatgcc
     1741 tccggtaagg gaatagttaa tatttcaacg gatagtctct ggaatttaaa gacgtcttct
     1801 actaacgccc aattgctaca ggtcggtgta ttaggcacgg gtgaactgaa tattaccacc
     1861 ggaggtatag ttaaagcgcg tgatacacag atagctctca atgacaaaag taagggcgac
     1921 gtgagggtgg atgggcagaa ctctcttctt gaaacattca atatgtacgt agggacatct
     1981 ggtacgggta cgttaaccct gacgaatagc ggcacgctga atgtcgaagg tggagaagtt
     2041 tacttaggtg tttttgaacc tgctgtagga acgctaaaca ttggtgcagc tcacggtgag
     2101 gcggcggcag atgccggatt tatcaccaac gcgacgaaag tagagtttgg ctctggcgaa
     2161 ggtgtttttg tctttaatca tactaataac agtgatgccg gctaccaggt cgatatgctg
     2221 attacaggtg acgataaaga cggaaaagtg atccatgatg caggccatac ggtgttcaat
     2281 gcagggaata cttatagcgg taaaacgctg gtcaatgacg gcctcctgac cattgcgtct
     2341 catacggcag atggggtaac gggcatgggg tcgagtgaag taaccattgc aagccccggt
     2401 acgctcgaca ttctcgcatc aacgaacagt gcaggagatt acacgctgac caatgcgctc
     2461 aaaggcgatg gcttgatgcg agttcagctg tcatcctccg acaagatgtt tggctttaca
     2521 catgcaacag ggactgaatt cgccggtgtt gcccaactga aagacagtac cttcactctg
     2581 gaacgcgaca acaccgctgc gcttactcac gcgatgttgc agtctgacat tgaaaatacc
     2641 acatcggtaa acgtgggaga gcaatccatt ggtggactgg ccatgaatgg cggtacgctc
     2701 attttcgata cggatattcc tgctgcgacg cttgcagagg gatatatcag cgtcgatacg
     2761 ctggttgtcg gcgcgagtga ctacacctgg aaaggccgta actatcaggt aaacgggacg
     2821 ggcgacgtgc ttatcggcgt gcctaaaccg tggaatgatc ctatggcgaa taaccctctg
     2881 acgacgctca atttgctgga acacgatgat aaccatgtcg gcgttcaact ggtgaaggcg
     2941 caaacggtta ttgggtcggg tggctcatta acgttacgtg atttacaggg cgacgaggtg
     3001 gaagcggaca aaacgttaca cattgcgcaa aacggaacgg tggtcgccga gggtgattat
     3061 ggattccgcc tcacgaccgc accaggtgat ggtttgtacg ttaactatgg gctgaaagcg
     3121 ctgaacatcc atggagggca aaagctgacg ttagccgaac atggcggagc ctatggcgca
     3181 acggccgata tgtcggcaaa aatcggtggt gaaggggatc tggcaatcaa tacggtgcga
     3241 caggtttcgc tttccaacgg tcagaacgac tatcaggggg caacctacgt tcagatgggg
     3301 acattacgta ccgatgcgga tggcgcgctg ggcaacaccc gggaactgaa catcagcaac
     3361 gcagccatcg tcgatcttaa tggatcgacg cagacggtag agacattcac cgggcagatg
     3421 ggttcgactg ttttgttcaa agaagggtcg ctgacggtaa ataaaggtgg gatcagtcag
     3481 ggtgaactga caggtggcgg aaacctgaat gttacagggg taaagcagat atcccgctag
     3541 gcatgattag agaagaaaat gactttcgca tctcggttgc tggcgcacag gagaagacag
     3601 cactgctcag aataggcaat gactggtgca ttccgaaagg aataacgccg acgacgcaca
     3661 tcattaaatt accgattggg gaaatcaggc agcccaatgc gacgctcgat ctcagccaaa
     3721 gcgttgataa tgagtattac tgtctgctgc tggcgaaaga acttgggttg aatgtcccgg
     3781 acgcagaaat cattaaagcg ggaaatgtgc gcgcgttagc ggtcgaacgt tttgacaggc
     3841 gttggaatgc tgagcgaacg gttttacttc gcttgccaca ggaggatatg tgtcagacat
     3901 tcggtttacc ttcatcggtg aaatatgaat cagatggagg cccaggcatc gcgcggatca
     3961 tggctttttt gatggggttc agcgaggcgc ttcgcgatcg ttatgatttt atgaaattcc
     4021 aggtcttcca gtggttgatt ggcgcaacgg atggccatgc aaaaaacttc tccgtattta
     4081 ttcaggctgg cggcagttat cgactcacgc cattttacga catcatttca gcatttccgg
     4141 tccttggcgg tacgggaata cacatcagcg atctcaaact ggcaatgggg cttaacgcat
     4201 ccaaaggcaa aaaaacggca atcgataaaa tttatccgcg acattttttg gcgacagcaa
     4261 aggtgctgag attcccggaa gtgcagatgc atgaaatcct gagtgacttt gccagaatga
     4321 ttccggcagc actggataac gtgaagactt cattaccgac agattttcca gagaacgtgg
     4381 tgacggcagt tgaaaccaat gtgttgaggt tgcacggtcg gttaagccga gaatacggta
     4441 ttaagtaaga gtaagatatg gggtttggtc gttgttaatg agcaggacaa tcatgaccgc
     4501 caacgactag attctccctt ggtttatatt ggtgcgcata atgtatatta tgttaaatcg
     4561 tatctgagtg atacgaagtg attttccggt agtcgttatt cgttcaggat tccagaactt
     4621 aagcttctcc tctttcataa ttggcctgtg catgacggat ataggctggc tggtatgccg
     4681 atttgccgtt tgccgcatct tcccagcgtt gaagactcaa gctggcaaac cgcgtgcaaa
     4741 tgagcacgcc ctctttccat caacacttca ggcagcttgc gggaatgagt ccactccggt
     4801 atcagaaatg gctacgtctc aatgaagtca ggcgaacgat gctgaatgaa cattacgatg
     4861 tcaccacagc agcctatgct gtcggttacg aaagcttatc ccatttccga cgggaatatt
     4921 tgcggatgtt tggagagtca cctaagagag atattaccag gttaagaaag tctgtcggtc
     4981 agctttaata aaccctgcac ttatctgttt agagcaattc ggtgttagtt acaccaggca
     5041 aacattaact atagctaatg atttatagcc atataaacca ttagtagccc ttgacctttc
     5101 ctgacacttc ccttgttcgc aataatattc tgtttagtca cggtttataa ataacatgca
     5161 taatgaaatc gtcatgttat ttagcgacat aaaatgtttt tgcaaatact ctcttcaaca
     5221 agtgcagcaa taaacttgtt gtgattaggt gtccttgtga tttaaagata ttatcgttct
     5281 tcgtgaaatg taataaccct ggaaactata ttctcagggc aacaaccaat caagaatgat
     5341 tcttgtttta taagcaataa cagaatgact taattgttaa ctctctttat tcagctactt
     5401 aaatataaat tttggagaat tatgaaactc aaacatgttg gtatgattgt cgtttctgtg
     5461 ttggcgatgt cgtctgctgc ggtaagcgca gccgagggtg atgaatcagt aacgaccact
     5521 gttaatggcg gtgttattca ttttaaaggt gaagtggtaa atgccgcttg tgcgattgat
     5581 tccgaatcaa tgaaccaaac ggttgagctg ggtcaggttc gttcttctcg cctggctaaa
     5641 gcgggtgacc tcagctccgc cgttggcttc aatatcaagc tgaatgattg tgataccaat
     5701 gtttccagta atgcagctgt tgcattcctg ggtactactg tcaccagtaa tgacgatacg
     5761 ttagcgctgc agagttcagc ggcaggctct gcccaaaatg tcggtattca aattttggac
     5821 cgtacgggtg aggtattaat acttgatggg gccactttta gtgctaaaac cgacttgatt
     5881 gatggcacga atatactacc attccaggct cgttatattg ctctcgggca gtccgtagct
     5941 ggtactgcaa acgcagatgc gaccttcaaa gttcaatatc tataattcaa tattaaggtg
     6001 gctatacggc atctgaaatt aaaacagggt gtgtgtggca tggctacgca cacccgagca
     6061 attcaccaat gtgaatctca ttattaatga tgagtggttt gtatttggag cgctatgaaa
     6121 aatcacataa tatctaaacg ttcaataaac aacagtagtt aaacttactg ttattaaaaa
     6181 tatttacttt ttagcaaggt tttcctatta aaagagaacc aacctttatt acaaacgcat
     6241 taagtctgga aacccggtga ctcgccaatg tattagcaac atttaccgat tgtctgatgt
     6301 aacggttttc ccgcttatcc tcttaacaca tggagaatgc atcagatgca aactacgcgt
     6361 accccctact ctatttcctt tatggcgaca gtgttacttt tattgctctt tgcttgccat
     6421 agcaccgttg ccaacgcagc tgtcgcactg ggtgcgacgc gggtaattta tccggcaaac
     6481 cagaaacagg ttttattgcc tgtcacgaat aatgatcctg ccagtgtcta tctcattcag
     6541 tcatggatag aaaatgctgg tgaccaaaaa gacacccagt ttgtcattac cccacctctt
     6601 ttttccatgc aaggtaaaaa agagaatacg ctgcggataa tcaacgccac taatcatcag
     6661 ttacccggcg accgtgaaag cctgttctgg gttaatgtta aagctattcc tgctatggaa
     6721 aaagaccaga agaatgagaa tacattacag ctggcaatta ttagccgcat aaaaatgttt
     6781 tatcggccca cgaacctggc aatggcacct gaagaagcgc ctgcaatgct tcggtttcgt
     6841 cgcagcggta gcaatctcac tcttattaat ccaacgccct acttcattac cgtaacaaat
     6901 atgaaagcag gaaatagcaa tctcccaaac accatggtgc cgccaaaagg tgaagtgtcg
     6961 gtggatatcc cgcatgctgc caccggggat atcagttttc agacgatcaa tgattacggc
     7021 gcattaactc cgcgtattaa ggcaaccatg cagtaagcca gtgacctaat taataataaa
     7081 ctcaatcgga attaataatg acagcctttc gtgccgcatt taaggcatat cgtatgcatc
     7141 aggtgttact actgcctcgc tttgcacggc ttaccattgc ccttagctta gcaactgcgg
     7201 tatttcccgt tgacgcagag tattatttta atccacggtt tttaagcaat gatcttgctg
     7261 aatctgtcga tttatccgcc tttactaagg gacgcgaggc accacctggt acatatcggg
     7321 tcgacattta tttgaacgac gaatttatga ctagtcggga tattactttt attacagatg
     7381 ataataacgc agatctcatc ccatgcctga gtacagatct tcttgtcagt cttggcataa
     7441 aaaaatctgc gttattagac aataaggaac attcagcaga aaaacatgtg ccggacaaca
     7501 gcgcatgcac accacttcag gatcgtttgg tcgacgcatc aactgagttt gatgttggtc
     7561 agcaacatct gtcactctcc gttccgcaaa tttatgttgg cagaatggct cgcggctatg
     7621 tttctcctga tctgtgggaa gaaggaataa atgctgggct tctaaactat agttttaatg
     7681 gtaattctat taataatcgt agtaaccata atgcaggaaa atccaactat gcatatttga
     7741 atttacagag tggcatcaac attggtagtt ggcgactacg cgataactca acgtggagtt
     7801 ataacagtgg gagtagcaat tcatctgaca gcaataaatg gcagcatatc aatacgtcgg
     7861 ctgaacgtga cattattccc ttacgctcac ggttaacggt aggtgatagt tataccgatg
     7921 gcgatatttt tgatagtgtg aactttcgtg gcctcaaaat aaattcaaca gaagcgatgt
     7981 tgcccgatag ccaacatggt ttcgctccgg tgattcatgg tattgcccgt ggcaccgcac
     8041 aagtgagtgt aaaacagaac ggatacgatg tttatcagac tactgttcca cccggccctt
     8101 ttactattga tgatatcaac tctgcggcca atggtggtga tctgcaagta accataaaag
     8161 aggcagacgg cagtattcag acattatatg ttccttattc gtctgttccg gttctccaac
     8221 gtgctggata tacgcgttat gcgcttgcca tgggggaata tcgtagtgga aataacctgc
     8281 aaagctcccc caggttcata caaggtagct tgatgcatgg actggaagga aactggacac
     8341 cttatggcgg aatgcaaatt gcagaagatt atcaggcctt caaccttggt attggtaaag
     8401 atttaggact ttttggtgcc ttttctttcg atatcacgca ggccaatacg acacttgcag
     8461 atggcacccg tcacagcggg caatcggtta aatccgtcta cagcaaatcc ttctaccaga
     8521 caggaaccaa tatccaggtc gcaggatatc gctattctac gcaaggtttt tataacttat
     8581 ccgacagtgc ctacagtcga atgagtggtt acaccgtcaa gcctcctacc ggagacagca
     8641 atgagcagac acaatttatt gattatttta atctgttcta cagtaagcgt ggtcaggaac
     8701 aaataagcat ctctcagcag cttggaaatt acggtgcgac atttttcagt gccagtcgcc
     8761 aaagttactg gaacacgtca cgcagcgacc agcaaatatc atttggatta aatgtgccgt
     8821 ttggtgatat tacgacttcg ctgaattaca gctattccaa taatatatgg caaaacgatc
     8881 gggatcattt actcgctttt acgcttaatg ttcccttcag tcattggatg cgtacagaca
     8941 gtcagtcggc atttcgtaat tcaaacgcca gttacagtat gtcaaacgat ttgaaaggcg
     9001 gcatgaccaa tctatcgggg gtttatggca ctctgctgcc ggataataac ctgaattata
     9061 gcgttcaggt cggtaacacc cacggaggta atacatcgtc tggcaccagt ggttacagta
     9121 ctcttaatta tcgtggagct tacggcaata ctaatgtcgg ttacagtcgg agtggtgaca
     9181 gcagccagat ttattacgga atgagtggtg ggattattgc tcatgctgat ggcatcacct
     9241 ttggacagcc gctgggcgac acaatggttc tggttaaggc tcctggcgct gataatgtca
     9301 aaatagagaa ccagaccgga attcataccg actggcgtgg ctatgccata ttaccatttg
     9361 cgacagaata tagagaaaat cgtgtcgctc ttaacgcgaa ttcccttgca gataatgttg
     9421 aactggatga aaccgtggtc actgtcatcc caactcacgg tgctattgcc agagcaacat
     9481 ttaatgcaca aatcggcggg aaagtattaa tgacgttgaa gtacggtaat aaaagcgttc
     9541 cattcggtgc aattgtcact cacggagaga ataaaaatgg cagcattgtc gcggaaaacg
     9601 gtcaggttta tctgactgga cttccacagt cagggaaatt acaggtttca tggggcaatg
     9661 ataaaaactc aaactgtatt gtcgattaca agcttcctga agtctctcct ggaaccttgc
     9721 tgaaccagca gacagcaatc tgtcgctaag gaacaataga ttatgaaata caataacatt
     9781 attttcctcg gtttatgtct ggggttaacc acctattctg ctttatccgc agatagcgtt
     9841 attaaaatta gcgggcgcgt cctcgattat ggctgcacag tctcatcgga ttcgcttaat
     9901 tttaccgtag atctccaaaa aaacagtgcc agacaatttc caacgaccgg tagcacaagt
     9961 ccagccgtcc cttttcagat tacgttaagt gaatgcagca aagggacaac gggggttcgg
    10021 gttgcattta acggtattga ggacgcagaa aataatactc tgttgaaact ggatgaggga
    10081 agcaatacgg cctccggttt aggtatagaa atactggacg gaaatatgcg tccggtgaaa
    10141 ctgaatgacc ttcatgccgg gatgcagtgg atcccactgg taccagaaca gaacaatatt
    10201 ttgccttact ccgctcgtct gaagtcaact cagaagtccg tcaatccggg actggtgagg
    10261 gcttcggcaa cctttaccct tgaatttcaa taatcagagt cagatatgaa aagacttcac
    10321 aagaggttcc tcttagctac gttttgcgcg ttattaacag caactctcca ggccgccgat
    10381 gtcactatca ctgttaatgg tcgggtagtc gctaaaccct gcactattca aaccaaagaa
    10441 gctaacgtta atctcgggga tctttatacg cgcaatctgc aacaacctgg ttctgcatct
    10501 ggctggcaca atattacttt gtcattaacc gattgtccgg ctgaaacaag tgcagtgacg
    10561 gcaatcgtga caggttcaac tgacaatacg ggttattaca aaaatgaagg tactgccgaa
    10621 aatattcaga tagagcttag ggatgaccag gatgcgacgt taaaaaatgg cgatagcaaa
    10681 acggttattg ttgatgagat cactcgtaat gcacagtttc cacttaaggc aagagctatc
    10741 acggtgaatg gaaacgcaag ccagggaacg atcgaggcgc taatcaatgt gatctacacc
    10801 tggcaataac gtcgattcga ttttcttagc cttcttttta aatcaatttt ttaaccttag
    10861 gatatatcat gggtaaaaca atcagtataa aggtcctgtt cggtatttat cttctattaa
    10921 tggcaggcaa agtttttgcc ttcagttgta atgtcgatgg cgggtcaagt attggagctg
    10981 gtacaacctc tgtttatgtt aatctcgacc ctgtaataca gccgggccag aatctggttg
    11041 tagacttgtc tcagcatata agttgctgga atgattacgg cggctggtac gacactgatc
    11101 atataaacct ggtacaaggc tcagctttcg caggctctct acagtcatat aaaggatcgc
    11161 tgtactggaa taatgtgacc taccccttcc cgttgacgac aaataccaac gtattggata
    11221 ttggtgataa gacaccgatg ccattaccgt taaaactcta tattacccca gtcggggcgg
    11281 ccggtggagt tgtcataaaa gccggggaag ttattgcccg tattcatatg tataaaattg
    11341 ccacgttagg aagcggaaat ccgcgtaatt ttacgtggaa tattatttca aataacagtg
    11401 ttgttatgcc aacaggcggc tgcactgttg atagtcgtaa tgtcaccgtg aatctcccgg
    11461 actttcctgg aagcgcagaa atcccccttg gcgtatattg ctcaagcgaa caaaagctct
    11521 cattttatct ttcgggtact accactgaca gtgctagaca agtgtttgct aacacagcac
    11581 cagatgcaac aaaagcatcg ggagtgggtg tctctctgat gcgtaatgga aaaatactgg
    11641 ctacgggaga aaatgtctcc ctggggacag taaataaaag caaagtacct ctgggtttaa
    11701 gcgcaaccta tggacaaaca gggaacaaag tttcagccgg aaccgtccag tcagtcattg
    11761 gtgtcacttt tatctatgaa taattgtgat tttgacctta gtctatgcgc tccattcacg
    11821 aaaattgcgc ggataattat tttgtgaagg ctattatcct acacttgtaa gaaaatccgc
    11881 gcacaat
//
LOCUS       DAZFAB010000126        11855 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZFAB010000126 DAZFAB010000000
VERSION     DAZFAB010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11855
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.126"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            336..4319
                     /gene="ehaA"
                     /locus_tag="ACHJWA_004065"
     CDS             336..4319
                     /gene="ehaA"
                     /locus_tag="ACHJWA_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HFO4310561.1"
                     /translation="MNKIYRLKWNRSRNCWSVCSELGSRVKGKKSRAVLISAISLYSS
                     LVFADDVIVNQDKTIDFGKENQSIDYRITVTDNANLVINATDTSRPRLTLASGGGLDI
                     TGGKVTINGPLNFLLKGTGFLNVSNAGSELYADDLYESNSGMRHDRGYFNVSNGGKIH
                     VKGTSRLTYLQGNVSGEGSQVNSETFFMGVYGSYGGNQYLSVNNGGEVNARKQISLGY
                     YDQVSDTTLAVSEGGKISAPTISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAKT
                     SEKKITLNHTDKDATISADIVSGSEGLGYINALNGTTYLTGDNSAFSGKVKIEQNGAL
                     GITQNIGTAEINNRGKLHLKADDSMTFANKISGNGTISIDSGTVELTGNNYAFSGYID
                     VASGAVAVISEDKNIGRAELDVDGKLQINANKDWVFDNDLEGRGIVEINMGNHEFSFD
                     EFAYTDWFQGSLAFQNTTFNLEKNAEFLQKGGITAGQGSLVTVGKGAHSISTLGFSGG
                     TVDFGALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVRSVSRDIDSALSLTEVDD
                     GNSTIKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTS
                     GVNNDGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKITGSGDLAFSSQKGQ
                     TVSLSNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNGHSQTVGTLNG
                     SADSLLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSAN
                     VLVSHAQGLGSANVENNGTLALNNSAEKRAAASVNYALGGNLTNNGTLMTGMSGQQAG
                     NVLVVKGNYHGNNGQLVMNTVLNGDDSVTDKLVVEGDTSGTTAVTVNNAGGTGAKTLN
                     GIELIHVDGKSEGEFVQAGRIVAGAYDYTLARGQGANSGNWYLTSGSDSPELQPEPDP
                     MPNPEPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGN
                     TYYTDMVTGEQKQTTMWMRHEGGHNKWRDGSGQLKTQSNRYVLQLGGDVAQWSQNGSD
                     RWHVGVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLDSWAQ
                     YSWFDNTVKGDDLQSESYKSKGFTASLEAGYKHKLAEFNGSQGTRNEWYVQPQAQVTW
                     MGVKADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLH
                     NSKDFSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAM
                     VGIKWQF"
     gene            4461..5549
                     /locus_tag="ACHJWA_004066"
     CDS             4461..5549
                     /locus_tag="ACHJWA_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO4310562.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMAGVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(5591..6522)
                     /locus_tag="ACHJWA_004067"
                     /pseudo
     CDS             complement(5591..6522)
                     /locus_tag="ACHJWA_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
     gene            complement(6614..7121)
                     /locus_tag="ACHJWA_004068"
                     /pseudo
     CDS             complement(6614..7121)
                     /locus_tag="ACHJWA_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
     gene            7379..7984
                     /locus_tag="ACHJWA_004069"
     CDS             7379..7984
                     /locus_tag="ACHJWA_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFO4310563.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNGDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            8024..8887
                     /locus_tag="ACHJWA_004070"
     CDS             8024..8887
                     /locus_tag="ACHJWA_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO4310564.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFRLIAWQQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVIDKQNI
                     ASAVASMMGLGIGLTPSADDYLTGLALILFISGHPAGKYKEEFYLGLQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            8877..10424
                     /gene="fdrA"
                     /locus_tag="ACHJWA_004071"
     CDS             8877..10424
                     /gene="fdrA"
                     /locus_tag="ACHJWA_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO4310565.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRGETPVDEQGLQFARGTKEAALKAVMLSGVKQEHLDLHTLNQPLITDVRA
                     RLKPQQKYIRGLFCGGTLCDETMFAVMEKHSDVYSNIQPDPEFRLKDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            10424..11842
                     /locus_tag="ACHJWA_004072"
     CDS             10424..11842
                     /locus_tag="ACHJWA_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO4310566.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQFDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPILRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVAQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAVDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
BASE COUNT         3132 a         2742 c         3198 g         2783 t
ORIGIN      
        1 aagagttacc acttttaccg gctgtatatt aaatgtattt ctcctgcttt tgtttatatt
       61 ttgagttgat ttgagttccc ggatacatat aatgcgcagg tattagagac tgggggggaa
      121 tgtaatgata aagttaagag tgttaactta aaataagttt ttagtagtaa cttgctattt
      181 tattaccctc tgaactacat actcagatat gtggttgcca atgctatgca aaatttttga
      241 atttttatat gcgatgaaca gtaaccggaa tggcatttaa tgcgttgctg tttatgcaat
      301 cttggtttta tttggatgta ttgctggaga ttgtgatgaa taaaatatat cggctaaagt
      361 ggaacaggtc ccgtaactgt tggagcgtct gctcggagct ggggagcaga gtaaaaggaa
      421 aaaagtcccg ggctgtttta attagcgcga taagtttata ttcatctctg gtattcgccg
      481 atgatgtcat cgtaaaccag gataaaacta ttgattttgg caaagagaac cagagcatcg
      541 attaccgtat tacggtgaca gacaatgcca atctggtaat caatgcgaca gatacttccc
      601 gtccgcgtct gactctcgct tctggtggtg ggttggatat taccggagga aaggtaacaa
      661 tcaatggccc acttaacttt ttgctgaaag gtacggggtt cctgaatgtc tccaatgctg
      721 gcagcgagtt atatgctgat gatttgtatg aatcaaactc aggcatgaga cacgatcgcg
      781 gctattttaa tgtctccaac ggcggcaaaa tccatgttaa gggcaccagc cgtctgacct
      841 atttgcaggg aaatgtcagt ggtgaaggta gccaggtaaa ttccgaaacc ttctttatgg
      901 gcgtttacgg cagttacggt ggtaatcagt acctgtcagt taataacggc ggtgaagtta
      961 atgccaggaa gcaaattagc ctgggctatt atgatcaagt ctccgataca acacttgctg
     1021 tttcggaagg tggtaaaatt tctgcgccta ctattagttt aagcaccaac tctgagttag
     1081 cgttaggggc acaggaagga agcgcagcga aggcagcagg gattattgat gccgaaaaaa
     1141 ttgagtttgt gtgggcaaag acatccgaga agaaaatcac cttaaaccac acggataaag
     1201 acgcgactat ttccgcggat attgtcagtg gcagcgaggg cctgggctat atcaatgcgc
     1261 tcaatggcac gacttactta accggtgata actctgcctt tagtggtaaa gtcaaaattg
     1321 agcaaaatgg cgctttaggg atcacccaaa atataggtac agcagagatc aacaaccgcg
     1381 ggaaattaca cctgaaggct gacgatagca tgacctttgc caataagatc tctggcaacg
     1441 gtacaataag tatcgacagt gggacggtgg agttgaccgg caataactat gcattcagcg
     1501 gatatattga tgttgcttct ggtgctgtcg ctgttatttc tgaagacaag aatatcggtc
     1561 gtgcagagct ggatgtcgat ggcaaattgc aaattaatgc caacaaagat tgggtatttg
     1621 ataacgatct tgaaggtaga ggcattgttg aaataaacat ggggaatcac gaattctcct
     1681 tcgatgagtt tgcttataca gactggttcc agggttcact ggcgttccag aacacgacat
     1741 ttaatctgga aaagaatgct gagtttctgc agaaaggcgg gatcactgcg ggtcagggaa
     1801 gcctggtaac agtgggtaag ggcgctcact ccattagcac tttgggattc tccggcggaa
     1861 ccgttgattt tggtgccctg acagcaggtg cacagatgac agaagggacg gtcaacgtta
     1921 gtaaaacgct ggatttgcgc ggcgagggtg tgattcaggt ttctgacagt gacgttgtcc
     1981 gctcagtatc tcgtgatatt gactctgcgt tatcgctcac tgaagtcgat gatggtaaca
     2041 gcaccattaa gttggttgat gcgcaaggtg cggaagttct gggcgatgcg ggcaatctgc
     2101 aattgcagga taaaaatggg caaatcctct ccagcagcgc ccaacgtgat attcagcaga
     2161 atgggcaaaa agcggccgtc ggcacttacg actatcgtct gacgagtggg gtaaacaatg
     2221 acggtctgta tattggttac ggcctgaccc agcttgattt acacgctacc gacagcgatg
     2281 ctctggtgct gagctctaac ggtaaaagcg agaatgccgc cgatctcagc gcaaagatta
     2341 ccggcagtgg tgacctggca ttcagcagcc agaagggtca gaccgtatcg ctttctaaca
     2401 aagacaacga ctataccggt gttaccgatc tgcgcagtgg gacgcttttg ttgaataacg
     2461 ataacgtgtt gggtaatacc catgaactgc gtctggcggc agagactgaa ctggacatga
     2521 atggtcacag ccagaccgtg ggcacgctca atggcagcgc cgattcactg ctgagcttaa
     2581 atggcggcag tctgacggtt accaacgggg gcacttcaac cggttcgtta acggggagcg
     2641 gagagctgaa tattcagggc ggcacgctgg acatcgcggg cgataacagc aacctgacgg
     2701 cgaatgtgaa cattgctaat tcggctaatg tcctggtaag tcatgcgcag ggattgggta
     2761 gcgcaaacgt tgagaacaac ggtaccctgg cgttgaataa tagcgctgaa aaaagagcgg
     2821 ctgcgtctgt gaattacgcc ctgggcggca atctgaccaa caacggtacg ctgatgaccg
     2881 gaatgtcagg acagcaagct ggcaatgtgt tagtggtgaa ggggaactac cacggtaata
     2941 acggtcaact agtaatgaat acggtactga atggcgatga ctcagtaacc gataaattgg
     3001 ttgtcgaggg cgatactagc ggcacgactg ccgttacggt gaataacgct ggcggtacag
     3061 gtgcgaaaac ccttaacggt atcgaactta tccatgtaga cggtaagtct gagggcgaat
     3121 ttgttcaggc tgggcgtatc gttgcggggg cgtatgacta cactctcgcg cgtggacaag
     3181 gggcaaatag tggtaactgg tatctgacca gcggcagtga ttctcctgaa ctgcagccgg
     3241 agccagaccc gatgccgaat ccagagccaa acccgaatcc agagccgaac cctaacccga
     3301 cacctacgcc gggtccggat ctgaatgtgg ataatgacct gcgaccggag gcgggtagct
     3361 acattgcgaa ccttgcagca gcgaatacca tgttcaccac gcgtctgcat gagcgtctgg
     3421 gtaatacgta ctataccgac atggtgacgg gtgagcagaa acaaaccact atgtggatgc
     3481 gccatgaagg tggtcataat aaatggcgtg atggcagcgg ccagctgaaa acccaaagca
     3541 atcgctatgt tctgcaactg ggaggcgatg tcgcgcagtg gagccaaaac ggcagcgacc
     3601 gctggcatgt tggggtcatg gcgggatatg gcaacagcga cagcaaaacc atttcctcgc
     3661 gaaccggtta tcgtgcaaaa gcgagtgtga acggatatag cacaggcctc tatgccacct
     3721 ggtatgccga tgacgagtcg cgtaatggcg cgtatctcga cagttgggcg cagtacagct
     3781 ggtttgataa cacagtgaaa ggggatgact tacaaagtga atcctataaa tcaaaaggat
     3841 ttaccgcttc actggaagct ggatacaaac acaaattagc tgaatttaat ggcagccagg
     3901 gaacgcgtaa tgaatggtat gttcagccgc aagcacaggt tacctggatg ggagtcaaag
     3961 ccgataagca ccgcgaaagc aacggaaccc tcgttcatag caacggtgat ggcaatgttc
     4021 aaacccgact tggcgtaaaa acctggctga agagccacca taaaatggat gacggtaaat
     4081 cccgcgagtt ccagccgttt gtagaagtga actggctaca taacagtaag gatttcagca
     4141 ccagtatgga tggcgtgtct gtcactcagg atggagcccg aaatattgct gagataaaaa
     4201 ccggggtgga aggacagcta aatgccaacc tgaatgtctg ggggaatgtg ggcgttcagg
     4261 ttgccgatag gggatataat gacacctctg caatggttgg cattaagtgg caattctgat
     4321 ctgaataatg agtaaaagga gagagagccg aacgggagat aattcggctc tctgtgtcat
     4381 tttcttgcag ccagctttat ccgtcagagt gaagactcgc agatttagct ggcgcacaag
     4441 atttaatcaa ggtgtgttca atggcttcgc atgatttgag tgtttttttg gaagagttcg
     4501 gggcaacggt gaatttgacg ttgcctggta tcgtgtccga aaaagaacgg cttttactca
     4561 agctgctaat ggagggaatg tcggttacag aaatctcaca gtacagaaat cgcagtgcaa
     4621 agacaatttc acatcaaaag aagcagctgt atgaaaaact aggcatccag agtgatatta
     4681 ccttctggcg ggatatcttt tttcaatatc atccgcaagt cattagtggc acgggaaata
     4741 aaaataattt ttatatccct gataaccgtt gtcaccatat cgtcacgcct gaagccatca
     4801 gtctggcgtt ggaaaaccat gaatttaaac cgtggatcca accggttttc tgcgcgcaga
     4861 ctggggtact gacgggctgt gaagtgcttg tccgctggga acatccacaa acgggaatca
     4921 tcccgccgga tcagtttatt cctctggcgg agtcatccgg tcttattgtc ataatgaccc
     4981 gccaactgat gaaacagact gcggatattc tgatgccggt aaaacatttg ctgccggata
     5041 atttccatat tggtatcaac gtctcggcgg gttgtttttt ggccgcggga tttgaaaaag
     5101 aatgcctgaa cctggtcaag aaattaggaa acgataaaat caaactggtt ctcgagctaa
     5161 cggaacgtaa ccctattccg gtaacgccag aagccagagc gatatttgac agccttcatc
     5221 agcataacat tacctttgcg ctggatgact ttggtacggg ttatgcgacc tatcgttatc
     5281 tgcaggcgtt tccggtcgat tttattaaga tcgataagtc atttgtgcaa atggcgggtg
     5341 tcgacgaaat atccggtcat attgtggaca atattgtcga actggcgcgt aagcctggtc
     5401 tgagtatcgt ggcggaaggg gtagaaaccc aggagcaggc ggatttaatg atcggtaaag
     5461 gcgttcactt tttgcagggc tatttgtact ctccgccagt accgggtaat aaatttatct
     5521 ctgaatgggt aatgaaagca ggtggttgat gtaaaccgct attcacagcg catcgggagg
     5581 ttggcagcga ttaattctcc cgatgcagta aatcctgata gatccccgtt aatatgccat
     5641 ttggcgcaaa ttgtttttta atccactgcg tcacctgacc cgtagcagaa tgctgagtcg
     5701 ccagtaacat gcgtgaatcc tggcgcgggt tatggatttg tcgggtaacc agcagggatt
     5761 gcgccatcgc ctcacggacc atgtaatctg gcaaaaaaac aatcccttca cctaaaatct
     5821 ggcactgaca tttggtgttg aaatctggca ccagaattga ctcctgcccg tgcagcaacc
     5881 agcccacttt tttattaatc gtatgcgcgg tgtcctccac cataatatta gggtagagac
     5941 gcagttggct ttcggcgatg gttccggcac aaaggctaac gggtgatccg gggcgatagc
     6001 aaaagcccag cgaatcgcgc caatttcagt gtaatcaata ccgccgccgt ccagcagcgt
     6061 gtcgggtgcg ccgatagcga tatttgcctg attattaata atcgcatccc agacgccgtt
     6121 atacacttcg gtggtgacgg taatctggca ggtaggaaac tgctttttca gcacctgtaa
     6181 taagcgtgcg gtgtgtttgg gcgtataaag caactgatta atacaaatgc gcacacgcgc
     6241 ttctatgccc tgcgaaatag tatcgatgct gcgtttgatg gcgtgaaaat cattcagcag
     6301 gtcggtagct ttgcggaaaa aataacgccc ggactccgtt aactcaatgc tgcgggtact
     6361 gcgagtgaac agcaccacat ccagccctgt ttccattcgc ttgatggtgt agctaatggc
     6421 ggaagtggtt aaacccaact cttctgcggc tttactgaaa ctgccaaaac gcgcagcggt
     6481 agtaaatgcc aacagattct cttcggtaaa aattgagttc attaaaccat tcccgccatt
     6541 atctaatatg aatttctcca agcataactg tagaggtatc aattagagat gggtaatcat
     6601 ttttgaataa cttttaatac ccgtcgcgtt tagtcgtcgc ttcgcttaat ttttgtccgc
     6661 catattctga aattatcccc acgcagttac ccgccagaaa acccagtaac gtcaccgtcc
     6721 agttcatccc gctggcaaaa aacagcgtca tgccgagaaa gccaccggga ataaatgaca
     6781 gtaaccagaa acgcccttgc cagaccacca ccgccgagaa gggtacggta gtaattacgc
     6841 tcgcccagaa cgcggacagt ccacttacag aggccagcca accggcagcc agcgcggcga
     6901 aaaacgccca caccatcccg gaataattaa ccgacagact tctgataaac cccgatttac
     6961 cgccgcctgc ggcataaaaa ctgcaaaagg cgacgaagtc aatggttcct aataattcct
     7021 aataattccc atccctgaga gagcccgaca tgtgaagaca ctaactgcca aagccctgca
     7081 caaataccga cggtgacacc cgttgccgtg agtccgttca tcttgttccc ctctggttag
     7141 acatggacga aagctactct tacatgtgca gactatagag aaataaaacc tgattgagtg
     7201 aaagggtatc gggtcaaaga aacaaatatt gcactaccgc acactgcgaa aagattgttg
     7261 aacatcattc aacaaaaagg cgaaatacgg cgaattaaat ttaagcgggt tcttttatca
     7321 gattatcgtt gctattctca attcatcgaa aacagccaca aaaaccaggg gaatgtgaat
     7381 gagtataaaa aatctacctg ccgactattt attggctgca caacagggtg atatcgataa
     7441 agtaaaaacc tgccttgcgc tgggtgtcga tattaatacc tgcgatcgtc aggggaaaac
     7501 ggcaattacg ctggcaagtt tatatcagca atatgcttgc gttcaggcat taattgatgc
     7561 cggagcggat attaataagc aagatcatac ctgtttaaat ccttttttaa ttagctgtct
     7621 gaacggtgat ttaacgctgc tacgaattat tttaccggct aaaccagacc ttaattgcgt
     7681 cacccgtttt ggcggtgtcg gcctgacgcc tgcctgtgaa aaaggccatt taagtattgt
     7741 aaaagagctt ttggcgcata cggaaattaa cgttaaccag accaaccatg ttggctggac
     7801 gccactactg gaagcgattg tgcttaacga tggcgggatt aaacagcagg cgattgtgca
     7861 gttattgctg gaacacggtg ccagcccgca tctgaccgat aaatatggca aaacgccact
     7921 ggaactggcg cgggaacggg gctttgaaga gattgcgcag ttactgattg ccgcaggtgc
     7981 ataaaccggg aggcttgcta tcaacacacc agaaaaacgg tgtgtgtggg cgctaactgc
     8041 ggatgcggat tttctggcgc agcgagggca aggacaggtt gaacaggtct ttgccagagc
     8101 ggtaaatatc gcactcccgg ctcgccagca gttgctgacg ctgctttgtg aagagtacga
     8161 caatgcgcca aacagttgtc ggttggcact cactcacttt gatgatctgt tccggcatgg
     8221 tgataaggtt cagtttgacg atcaaggtat tacggttggt caacatcttc atatagagat
     8281 gagtcgttgt cggcgttggc tgtccccaac cttgcaaatg accgctgtga attttcgcct
     8341 tatcgcctgg caacagtggc acgacatcat tcatcagcac ctgggggaaa atgaaaccct
     8401 gtttaattat cgcggcgata atccgtttta tcaggcgtta aataaagaat tacatattaa
     8461 acgacgggca gttatacagg cagtaatcga taaacaaaat atcgcctcag cggtcgccag
     8521 tatgatgggc ttagggattg gccttacgcc atcagccgat gattatttaa caggtctggc
     8581 acttatttta tttatttccg ggcatccggc gggaaaatac aaagaggaat tttatctcgg
     8641 cttgcaacgc ggcagaaata ataccacgtt attaagtgcc ataacgctgg aagccgcatt
     8701 acaacaacgc tgccgggaaa atattcatcg ttttattcac aacattattt atgacatccc
     8761 tgggaacgca actcaggcaa tagaaaaaat taaacatatt ggctccagtt ccggctgcga
     8821 catgctgtat ggcatggccg atggttgtgc gctgagccaa acctacggag ggaattatgt
     8881 cagttaaaat agtcattaaa ccgaatacct attttgattc tgtctcgctg atgtctatct
     8941 ccacgcgtgc aaataaactc gacggcgtcg agcaggcatt tgtggcgatg gcgaccgaaa
     9001 tgaacaaagg tgtgctgaaa aatttaggac tgctgacgcc ggagctggag caggcgaaaa
     9061 acggcgacct gatgattgtc atcaatggta aatcgggggc ggacaacgag cagttgctgg
     9121 tggagattga agaactgttc aacaccaaag cgcaaagcgg ctcgcacgag gcgcgttacg
     9181 ccactattgc cagcgccaaa aagcatatcc cggaaagtaa cctggcggtg atttcggtca
     9241 acggtctgtt tgccgctcgc gaagcgcgtc aggcgctgca aaacgatctc aacgtgatgc
     9301 tgttttccga taacgtctca gttgaagatg aactggcgct caagcaactg gcccacgaaa
     9361 aagggctgct gatgatgggg ccagactgcg gcacggcgat tatcaacggt gcggcgctct
     9421 gctttggtaa cgccgtgcgt cgcggcaaca tcggtattgt tggcgcatcc ggcaccggca
     9481 gtcaggagtt gagcgtccgc attcatgaat ttggcggcgg cgtttcgcaa ctgattggca
     9541 ccggcgggcg cgacctgagc gagaaaatcg gcggcctgat gatgctcgac gccatcggga
     9601 tgctggaaaa cgatccgcaa actgaaatca ttgcgcttat ctccaaaccg cctgcgcctg
     9661 cggtggcccg caaagtgctg gaacgtgcgc gcgcctgccg caagccggtg gtcgtttgct
     9721 tcctcggtcg tggcgaaacg ccagtggatg agcaggggct acagtttgcc cgcggcacca
     9781 aagaggcagc gctaaaagcg gtgatgctct ccggcgtgaa acaagaacat ctcgacctgc
     9841 ataccctgaa ccagccgttg attactgacg tgcgcgctcg cctgaagccg cagcagaaat
     9901 acattcgtgg cctgttctgc ggtggcacgc tgtgcgatga aacgatgttc gcggtgatgg
     9961 aaaaacacag cgatgtctac agcaacatcc agcccgatcc ggaattccgc ctgaaggata
    10021 tcaaccgcag catcaaacac accttcctcg actttggcga tgacgacttc accaacggca
    10081 agccgcaccc gatgattgac cctaccaacc gcatcagccg cttgatcgaa gaggcgcgcg
    10141 atccagaagt ggcggtgatc gtgatggatt ttgtgctcgg atttggatcg catgaagatc
    10201 cggtcggttc caccatcgag gcgatcaaag aagcgaaagc gatcgccgct gccgaaggac
    10261 gcgagttgat cattctcgcc tatgtgctgg gtaccgatct tgatacgcca tcgttagaac
    10321 aacaaagcca gatgctgctt gatgccggcg tgattctggc gagcagcagc accaataccg
    10381 gattgctggc gcgtgaattt atctgcaaag gggaggaagc ctgatgagcc agtcactgtt
    10441 tagccaacca ttgaacgtta ttaacgtcgg catcgccatg tttagcgatg acctgaaaaa
    10501 gcagcatgta gaagtgactc aattcgactg gacgccgccg gggcagggca atatgcaggt
    10561 ggtgcaggcg ctggataaca ttgccgattc gccgctggca gacaaaatcg ccgccgctaa
    10621 ccagcaggcg ctggagcgca ttatccagtc gcatccggtg ctgattggtt ttgatcaggc
    10681 gattaacgtg gtgccgggca tgacgccgaa aaccattctt cacgccgggc cgccgatcac
    10741 ctgggaaaaa atgtgcggcg cgatgaaagg cgcggtcacc ggagcgctgg tgttcgaagg
    10801 actggcgaaa gatctcgacg aggcggctga actggcggct tccggggaga tcaccttctc
    10861 gccgtgtcac gagcacgact gcgtgggatc gatggcgggt gtgacctcgg cctcgatgtt
    10921 tatgcacatc gtaaaaaaca aaacctacgg caacatcgct tataccaaca tgagcgagca
    10981 gatggcgaag attttgcgta tgggcgctaa cgaccagagc gtgattgacc gcctgaactg
    11041 gatgcgtgat gtgcagggac caatactgcg cgacgcgatg aaaattatcg gcgaaatcga
    11101 tctgcgctta atgctggcgc aggcgctgca tatgggcgat gagtgccata accgcaataa
    11161 cgccgggacg acgctgctga ttcaggcgct gacgccgggg attatccagg cgggctattc
    11221 cgtcgcgcaa cagcgcgaag tgtttgagtt tgttgccagt agcgattact tctccggccc
    11281 gacgtggatg gcgatgtgta aagcggcgat ggatgcggcg cacggcatcg aatacagcac
    11341 cgtcgttacc accatggcgc gtaacggcgt cgagttcggc ctgcgggtga gcggcctgcc
    11401 ggggcagtgg tttaccggcc cagcgcagca ggtcatcggc ccgatgtttg ctggttataa
    11461 gccggaagat tcggggctgg atatcggcga cagcgccatc acagaaacct acggtattgg
    11521 cgggtttgcc atggcgaccg cgcctgcaat cgtcgcgctg gtggggggca cggtggaaga
    11581 ggctatcgat ttctcccgtc agatgcgcga aatcaccctt ggcgaaaacc ccaacgtcac
    11641 cattccgctg ctcggtttta tgggcgtgcc gtcggcagtc gacatcaccc gcgtgggtag
    11701 cagcggcatt ctgccggtga tcaacaccgc catcgcccat aaagatgcgg gcgtcggcat
    11761 gattggcgcg ggcattgtgc atccaccatt cgcctgcttc gagaaagcca ttcttggctg
    11821 gtgcgaacgt tacggcgttt gacctacctg ttcat
//
LOCUS       DAZFAB010000127        11756 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZFAB010000127 DAZFAB010000000
VERSION     DAZFAB010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11756
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.127"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..250
                     /locus_tag="ACHJWA_004073"
     CDS             <1..250
                     /locus_tag="ACHJWA_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310567.1"
                     /translation="ILFSSVDDGPVMVVSSQRLHDMLNPTKDTNWNSTYIYKSRHEML
                     PVNLTPETLFGSKSYDKHALFPIFTASWRANRIKNKGI"
     gene            473..970
                     /gene="ubiC"
                     /locus_tag="ACHJWA_004074"
     CDS             473..970
                     /gene="ubiC"
                     /locus_tag="ACHJWA_004074"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFO4310568.1"
                     /translation="MSHPALTQLRALRYCKEIPALEPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            983..1855
                     /gene="ubiA"
                     /locus_tag="ACHJWA_004075"
     CDS             983..1855
                     /gene="ubiA"
                     /locus_tag="ACHJWA_004075"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFO4310569.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2010..4433)
                     /gene="plsB"
                     /locus_tag="ACHJWA_004076"
     CDS             complement(2010..4433)
                     /gene="plsB"
                     /locus_tag="ACHJWA_004076"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFO4310570.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPVHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4604..4972
                     /gene="dgkA"
                     /locus_tag="ACHJWA_004077"
     CDS             4604..4972
                     /gene="dgkA"
                     /locus_tag="ACHJWA_004077"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFO4310571.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5082..5690
                     /gene="lexA"
                     /locus_tag="ACHJWA_004078"
     CDS             5082..5690
                     /gene="lexA"
                     /locus_tag="ACHJWA_004078"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFO4310572.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5763..7088
                     /gene="dinF"
                     /locus_tag="ACHJWA_004079"
     CDS             5763..7088
                     /gene="dinF"
                     /locus_tag="ACHJWA_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFO4310573.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVRGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTSWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLASLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7204..7413
                     /gene="yjbJ"
                     /locus_tag="ACHJWA_004080"
     CDS             7204..7413
                     /gene="yjbJ"
                     /locus_tag="ACHJWA_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFO4310574.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7455..7970)
                     /gene="zur"
                     /locus_tag="ACHJWA_004081"
     CDS             complement(7455..7970)
                     /gene="zur"
                     /locus_tag="ACHJWA_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFO4310575.1"
                     /translation="MEKTTTQELLAQADKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            8289..9245
                     /locus_tag="ACHJWA_004082"
     CDS             8289..9245
                     /locus_tag="ACHJWA_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFO4310576.1"
                     /translation="MLKIIPGATGYFNKTLNSNQFDNEDAIKGKVNSIYGKSIDYSTM
                     RHRDIIIAKIDLFIQRITHNLWTAREKNVTLIKQINDLKMCVNEYIGDCTDEELNDRE
                     FIASVVDRAIFHFAINSICNPEDNKDATLIERCTFDVETKNGLPSTVQLFYEESKDNE
                     PLANIHLQAIGSGFLTFVNACQEHDDNSLKLFASLLISLSYSSVYSDFAGRVNINEYN
                     DNYLKAQFEELSQRDMKKYLGEMKRLADGGEMNFDGYLDKMSHLVNEGTLAPDILSKM
                     RDAAPKLIDFAKSFDPNSKEKIKIYTGTSKLIYDLFGVKSEK"
     gene            9607..9711
                     /locus_tag="ACHJWA_004083"
                     /pseudo
     CDS             9607..9711
                     /locus_tag="ACHJWA_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
     gene            9858..10391
                     /locus_tag="ACHJWA_004084"
     CDS             9858..10391
                     /locus_tag="ACHJWA_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310577.1"
                     /translation="MLTLDEIGQSVRNNIQLIIDHVGLPLAVGPLSDDDYKILCGGYG
                     ELEWDYALSTYGNSREKYEFCIKLVQQGRVQGIPSGAAICVYGVEENIFRIHMIERFS
                     REDESHPLKGRMVLLTLMSAFIFCKAVECKVVHIVEPVPELVQYYESFGFRMEQCGYV
                     MSAVIDELQDIFLKFAQ"
     gene            10588..10761
                     /locus_tag="ACHJWA_004085"
     CDS             10588..10761
                     /locus_tag="ACHJWA_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310578.1"
                     /translation="MKDQKATKPQVKFDTMKAFAGMGAAVEVLMKAAPNAFTHATVSG
                     KEQQGKLRRRKAA"
     gene            complement(10809..11090)
                     /locus_tag="ACHJWA_004086"
     CDS             complement(10809..11090)
                     /locus_tag="ACHJWA_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyocin activator PrtN family protein"
                     /protein_id="HFO4310579.1"
                     /translation="MNTLFLLMAEFNTPNIELSAVSQKYFGMSPATAEAKANACKLPV
                     PTYRIGTSQKAKRCINIQDLAEYIDKRREEGRIEWEQVRTVKQKDKEHH"
     gene            complement(11127..11477)
                     /locus_tag="ACHJWA_004087"
                     /pseudo
     CDS             complement(11127..11477)
                     /locus_tag="ACHJWA_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
     gene            complement(11435..11632)
                     /locus_tag="ACHJWA_004088"
     CDS             complement(11435..11632)
                     /locus_tag="ACHJWA_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310580.1"
                     /translation="MAELTKWLQNTITGIETVVDDKSFVCDEIVFKIDVVKNVLTAFK
                     VALVSLEVLMILCRSAMLSQN"
BASE COUNT         2883 a         2653 c         3013 g         3207 t
ORIGIN      
        1 tatcttgttc tcctctgtcg atgatgggcc agttatggtg gtaagctccc agcgcttaca
       61 tgatatgttg aatcctacaa aagataccaa ttggaattcc acgtatatct ataaatccag
      121 acatgagatg ttgcctgtta atcttactcc ggaaacactt ttcggctcca aatcttatga
      181 taaacatgcg cttttcccca tttttactgc gagttggcga gctaatcgta taaagaataa
      241 aggtatttaa gtaaaggaaa acatcaccgc tcttggcatc atgggcggtg atgcccctat
      301 ggttgccctc gccagcacgg gcatcggcaa agcgtaaggt tcaacagcgt tttaccactt
      361 catgcgattg ttgcgttttt gttgcgcatt agatcactta atttgcttta catctcccgt
      421 aaacactttt ctgcgataca atgcctttac gttatgtaac ggagagttcg gcatgtcaca
      481 ccccgcgtta acgcaactgc gtgcgctgcg ctattgtaaa gagatccctg ccctggagcc
      541 gcaactgctc gactggctgt tgctggaaga ttccatgaca aaacgttttg aacagcaggg
      601 aaaaacggtg agcgtgacga tgatccgcga agggtttgtc gagcagaatg aaatccccga
      661 agaactgccg ctgctgccga aagagtctcg ttactggtta cgtgaaattt tgttatgtgc
      721 cgatggtgaa ccgtggcttg ccggtcgtac cgtcgttcct gtgtcaacgt taagcgggcc
      781 ggagctggcg ttacaaaaat tgggtaaaac gccgttagga cgctatctgt tcacatcatc
      841 gacattaacc cgggacttta ttgagatagg ccgtgatgcc ggactgtggg ggcgacgttc
      901 ccgcttgcga ttaagcggta aaccgctgtt gctaacagaa ctgtttttac cggcgtcacc
      961 gttgtactaa gaggaaaaaa atatggagtg gagtctgacg cagaataagc tgctggcgtt
     1021 tcatcgctta atgcgtacgg ataagccaat tggcgcgtta ctgctgctct ggccaacatt
     1081 atgggcgttg tgggtggcga caccgggcgt tccccagctc tggatcctgg cggtgtttgt
     1141 cgcgggtgtc tggctgatgc gcgctgccgg atgtgtggtg aatgattatg ctgaccgcaa
     1201 gtttgatggt catgttaagc gcacggcgaa ccgaccactt cccagcggcg cggtaacaga
     1261 gaaagaggcg cgcgcgctgt ttgtagtgct ggtactgatt tcgtttttac tggtgctgac
     1321 gctgaatacg atgaccattc tgttgtcgat tgccgcgcta gcgctggcgt gggtgtaccc
     1381 gtttatgaag cggtataccc atctaccgca agtggtgctg ggcgcggcgt ttggctggtc
     1441 gattccaatg gcttttgctg ctgtgagtga gtcggtgcca ttgagttgct ggttaatgtt
     1501 cctcgccaat attctctggg cggtggctta cgacacgcag tatgcgatgg ttgaccgtga
     1561 tgatgatgtg aagattggca ttaaatccac cgcaatcctg ttcggccaat acgataaatt
     1621 gattattggt attttgcaga ttggcgtact ggcgctgatg gcgattatcg gtgagttaaa
     1681 tggcttaggc tggggatatt actggtcaat tctggtggct ggcgcgctgt ttgtttatca
     1741 acaaaaactg attgccaacc gcgagcgcga agcctgcttt aaagcattta tgaataataa
     1801 ctatgttggt ctggtactgt ttttagggct ggcaatgagt tactggcatt tctgatgatg
     1861 taaaaaagcc ggatgatcat ccggctttct tctgggttgc ctgatgcgct gcgcttctca
     1921 ggcctacaca acagatcgca atttattgaa tttgcagatt atggaaggcc ggataaggcg
     1981 tttgcgccgc atccggcaat tctctctgat tacccttcgc cctgcgtcgc actctcaatc
     2041 gtcaaacgca cgtctgatgt aatcaactcc gccagcaact gataaacctt catcgtttct
     2101 gccggttcgg catcgccgct atcgctgata tacccttcat cacgcagcgt aagcaccaga
     2161 gaactgaaca ccgccttgtc gaagaactcc ggcgcgttga tgccgtgcag cacggagaga
     2221 cgttgcgcga cggtgcggct ctctttctcc agcgtaccgc ggttgatcga cgggttggcg
     2281 ctcaacagcc agaaggtgat ggcataacgt tgcagcgttt cgcgcgcgcc tgcggcgagc
     2341 agctgtagcg tgcgagaatg caccgggttg atatgcaact catcatcttg cagggtaatc
     2401 agcccctgac gttgcatctc atttgccagc gcatcaataa cgtccggcaa ctcgtcgcga
     2461 tcccagcgca ggaacagctc cgctttcagc attgggtaaa gcacattgac gtgctccatc
     2521 aatacgtcgc gggagatgtg gcgatgctgg gtgacaattg ccgccatcag cgaaggtagc
     2581 accaacatat gcgcaatgtt gttgcgatag taggtcatca gcaccgcttg ctcgcgcggc
     2641 agaatgatga tgtcgccgat agtgtctttc tcaacttcaa acttgttcat ttgcagcgcg
     2701 tgatcgataa gctcgctggc gctggctgaa ggaacggtag agtccgtgga gtaaggcacg
     2761 ttgcgcatca gatccaggta gcagttgagt tgctcggtta actgctcgcg ggtgagtgag
     2821 cgctgacgtg atgccagtag cgcagtacag cacaggttca tggcgtttgc cgcgcctgcg
     2881 ttgttaatgc gtaccatcag atcggcagca atattattga ccgtcggcgt taaccatgcc
     2941 ggacgcactg cttcgatggg atcgatagat tcacgccagt ctggtacgtg ctggttaagg
     3001 taggtcatca acggcattgg ttcaccgaaa ttgacgtaac cctggccgag attacgcagc
     3061 ttgcttaaac cgcgcagcat ctgcggcagg ctctctttct ctttcgtcgc gccgcgcagt
     3121 tctttggcgt aagtacccac ttccatgacg tgctcataac cgatatagat cggaatcagc
     3181 gtaatcggac gcgtgccgcc acgcagcatc gcctgaatgg tcatcgacag cgtaccggtt
     3241 ttcggatcca gcaaacgccc cgtacgggaa cgaccgcctt ctacgaagta ctcgacggaa
     3301 taaccacggc tgaacagttc gccgagatac tcacggaaaa cggtggaata gagcttattg
     3361 cctttaaaag tacggcgaat aaagaacgca cccagacggc ggaaaatcgg cccggcaggc
     3421 cagaaattca ggttgatccc ggcggcgata tgcggcggca ctagcccctg gtggtacagc
     3481 acgtaagaaa gcagcaggta gtccatgtga ctgcggtggc aaggcacata taccagctca
     3541 tgaccgtcgt gggccagctg gcgaacgcgc tcggcgttat ggacgttgat gccctggtaa
     3601 aggcggttcc aggtaaagcc cagaatacgg tcagtcaggc ggatcatctc gtaagagaaa
     3661 ttcgccgcaa tctcttccat cagcgcaatc gcgttctgct gcgctttttc atgggagatt
     3721 tttttgctgc gcgcttcatc ttctaccgct ttggcaatgg cgcgggaggc gagcagctta
     3781 ttaaacagat cctgacgagc aggcagacgt ggtcctacgg cagccagacg ttgacgggca
     3841 aagtgcatac gcgccacgcg cgccagtttc tgcgcgatag ttttatccgt gccgtgttca
     3901 tccgccatac ggcgcagcga aactgacggc gagaaacgca caaaactgtc gcgaccgagc
     3961 cacagtacag cgaaaaattt ctgtacgccg ttaagcatac gcagcggcgg gttcacttcg
     4021 cctttttcac gccccggcgc gcgaccaaac atcaccgaca ctggcaccat ctgcacatcc
     4081 agatttgggt tgctacggtg caaatcgaga tagtcgtgga acagcttaat agactcttct
     4141 ttcggcgtgt aataggtgaa tacacgcggc ccgccgtgaa tgaacacata gcgcggcagt
     4201 agcgtgccgt cgatttccag cggctctaac gggtcaggca agtcatgtgc cagacactgg
     4261 gcgcgcaacg tcagcaaatc tgctttcgag ttgtacggta aaacgtacat aattggacga
     4321 gaggtatcca gccccagttc cggggcagga tctgccggaa tagacttgct ttttaccagg
     4381 atgcttaatg gtaaattcag taatttgtag taaattcgtg gccagccgga cataaacgat
     4441 gtaaagcctc tggttaataa tgcaaatgcg cggcaaggat agcagaaagt catgggaaat
     4501 tctgtggtat ccgctcatgt ttcgcgcggc gttacgcaaa cccgaatcat cggatttaac
     4561 ggtacactga tattgacgct cataatgtaa aaaggttctt tcaatggcca ataataccac
     4621 tggattcacc cgaataatca aagctgctgg ctattcctgg aaaggtttac gcgctgcatg
     4681 gatcaacgaa gcggcattcc gtcaggaagg cgtagcggta ttgttggcgg tggtcatcgc
     4741 ctgctggctg gatgtggacg cgattacccg cgtgctgctt atcagctccg tgatgctggt
     4801 gatgattgtg gaaatcctca atagcgccat cgaagcagtg gttgaccgaa ttggctctga
     4861 ataccatgag ctttccggac gcgctaaaga tatgggatcc gccgcggtgc tgattgcgat
     4921 tatcgtcgcc gtgattacct ggtgcatcct gttatggtcg cattttggat aacccttcca
     4981 gaattcgata aatctctggt ttattgtgca gtttatggtt ccaaaatcgc cttttgctgt
     5041 atatactcac agcataactg tatatacacc cagggggcgg aatgaaagcg ttaacggcca
     5101 ggcaacaaga ggtgtttgat ctcatccgtg atcacatcag ccagacaggt atgccgccga
     5161 cgcgtgcgga aatcgcgcag cgtttggggt tccgttcccc aaacgcggct gaagaacatc
     5221 tgaaggcgct ggcacgcaaa ggcgtaattg aaattgtttc cggcgcatca cgcgggattc
     5281 gtctgttgca ggaagaggaa gaagggttgc cactggtagg tcgtgtggct gccggtgaac
     5341 cgcttctggc gcaacagcat attgaaggtc attatcaggt cgatccttcc ttgttcaagc
     5401 cgaatgctga tttcctgctg cgcgtcagcg ggatgtcgat gaaagatatc ggcattatgg
     5461 atggcgactt gctggcagtg cataaaactc aggatgtacg taacggtcag gtcgttgtcg
     5521 cacgtattga tgacgaagtt accgttaagc gcctgaaaaa acagggcaat aaagtcgaac
     5581 tgttgccaga aaatagcgag tttaaaccaa ttgtcgttga ccttcgtcag cagagcttca
     5641 ccattgaagg gctggcggtt ggcgttattc gcaacggcga ctggctgtaa catatctctg
     5701 agaccgcgat gccgcttggc gtcgcggttt gtttttcatc tctcttcatc aggcttgtct
     5761 gcatggcatt cctcacttca tctgataaag cactttggca tctcgcctta cccatgattt
     5821 tctccaatat cactgttccg ttgctgggac tggtcgatac ggcggttatt ggtcatcttg
     5881 atagtccggt ttatttgggc ggcgtggcgg ttggtgcaac ggcgaccagc tttctcttta
     5941 tgctgttgct gtttttacgc atgagcacca ccgggctgac tgcgcaggct tatggtgcca
     6001 aaaatcctca ggcattagcc cgtgcgctgg tgcaaccttt gctgttggcg ttgggggctg
     6061 gggcgttaat tgcgctgctg cgtacgccga ttatcgatct ggcgctgcat attgttggcg
     6121 gtagtgaagc agtcctggaa caggcgcggc gctttcttga aatccgctgg ttaagcgcac
     6181 cggcgtcgct ggcgaatctg gtattacttg gttggttact cggcgtgcaa tatgcccgtg
     6241 cgccagtaat tttgttagtg gtcggcaata tcctcaacat tgtgctggat gtctggctgg
     6301 tgatgggact gcatatgaac gtgcggggcg cggcgctggc gacggttatt gcggaatatg
     6361 caacattgct gattggtctg ctaatggtgc gtaaaatcct caaactacgc ggaatctccg
     6421 gcgaaatgct gaaaacttcc tggcgaggaa acttccgtcg cttgctggca cttaaccgcg
     6481 atatcatgct gcgctcgctg ttgttgcaac tctgtttcgg cgcgatcacc gtacttggcg
     6541 cgcgactggg gagtgacatt atcgctgtta acgcggttct gatgacgcta ctcaccttta
     6601 ccgcctatgc gctggacggt tttgcctacg cggttgaagc acattccggt caggcatacg
     6661 gtgcgcgcga cggtagccag ttgctggatg tctggcgggc agcatgccgc cagtcgggga
     6721 tcgtagcgtt actgttttcg gtggtttatt tgctggcagg ggaacacatc attgcgttac
     6781 tggcgtcgtt aacccagatt cagcagctgg ccgaccgcta tcttatctgg caggtgattt
     6841 tgccgttggt cggcgtctgg tgttatctgc tggacggcat gtttataggc gcaacgcgtg
     6901 ctgctgaaat gcgtaacagt atggcggtgg ccgccgcagg ttttgcgctg acgctcctta
     6961 cgctgccgtg gctgggtaat catggtttgt ggttggcgtt aaccgtcttt ctggcgttgc
     7021 gcgggctttc tctggcagct atctggcggc gtcactggcg caacggtacc tggtttgccg
     7081 caacgtgacg gttaaaaatt ctgaataaat aatcctaagc caaattgctg actacactta
     7141 atctcacgtt cagaagaaaa gtgaacgtac tctcattcac aacctaacga tgaggtcttg
     7201 attatgaata aagatgaagc cggcggtaac tggaaacagt ttaaaggtaa agtgaaagag
     7261 caatggggca aactgaccga tgatgatatg acgatcattg aaggtaaacg tgatcaactg
     7321 gtcggtaaaa tccaggaacg ttatggttat cagaaagatc aggcagaaaa agaggtcgtg
     7381 gattgggaaa cccgcaatga atatcgctgg taattaatcc ctcctgcccg acgtgtacaa
     7441 ggatgtacgc cctcttaacg cggtttcttt ttcacctgca cagagtgatc atgctggcac
     7501 tgttcaggat gacgacacgc ttccacttcc acgcatgccg cacagagccc atgtgcttca
     7561 atcacattat gccgcagggc aaaccccatt tttgccgcca gcgtatgcat gatgtcttcc
     7621 acgccttcag cacattcttc tttcaccgcg ccgcagcgat cgcaaataaa catagctgac
     7681 gtatgggtgg gctggtcgaa cagatggcag agcacataac tgttggtgga ttccacctta
     7741 tgcacaaaac cttgctcaag cagaaaatcc agcgcgcgat aaaccgttgg cggcttggct
     7801 tgcggttcag cttcgcgcag caaatcaagc agatcataag cgctgatagc gccatcctgc
     7861 agactcatca ggcgcaacac ttccaggcgc tgtggggtca ggcgcacatt acgctgcgcg
     7921 cagattttgt cagcctgcgc taataactcc tgcgttgtgg tcttttccat cgggcacctc
     7981 aaagtggggg ttaagaatgt ccttacttta ccatgttcca ggaaaaacac cgagaaccct
     8041 tattgttgcc gtaatattgc ttttctgttt tgtaggtaag atgttgtgtt gcctggcagt
     8101 accattatca acacgataat aattaataat tcatttttaa ataaattgct tcaagttaac
     8161 tcgctattta tttaaccgtg gctaatcgta tacccgaatt tcagaaaatt atgagcgatt
     8221 tttgatagtt accggttcca aaaacactta taaggaggac acggtacgac aaaaaaggta
     8281 gatgaatcat gttaaagata atacccggtg caacaggata ctttaataaa acactcaatt
     8341 caaatcaatt cgataatgaa gatgcaatca aaggtaaagt aaatagtata tatggtaaat
     8401 ctattgatta ttcaacgatg cgtcatcggg atataattat tgcaaaaata gatttattta
     8461 ttcaaagaat cactcataat ttatggactg cacgtgaaaa aaatgtgacg ttaattaagc
     8521 aaataaacga tctcaaaatg tgtgtcaatg agtatattgg agattgtact gatgaggaat
     8581 taaacgatcg tgaatttatt gcatcagttg ttgacagggc tatttttcat ttcgcgatta
     8641 atagtatatg taatcccgag gataataaag atgcgacact catagaacga tgtacttttg
     8701 atgtagaaac caagaatggt cttccctcca cggttcagct attttatgag gaatctaaag
     8761 ataatgaacc tttagcgaat atacatttgc aagcaatagg ttctggtttc ttaacgtttg
     8821 ttaatgcctg ccaggaacat gatgacaaca gcttaaaatt atttgcttcg ctgttaattt
     8881 ccctttcata ttctagtgtc tactcagatt ttgcaggaag agtgaatatt aatgaatata
     8941 atgataacta cctgaaagct cagtttgaag aattatctca acgtgatatg aagaagtacc
     9001 tgggagagat gaagcgtctg gcagatgggg gagaaatgaa ttttgatggc tatctggata
     9061 agatgtcaca tctggtgaat gaaggaacgc tcgcccctga tattttaagc aaaatgcgag
     9121 atgctgcacc gaaattaatt gactttgcta agtcgtttga cccaaactca aaggaaaaga
     9181 ttaaaatata tacaggcact tctaaattaa tttatgattt attcggggtt aaatcggaga
     9241 aataatatgt gaagttcttc gatggtatga aaggtattac ataaaagaac ccaatgttta
     9301 ttgggttctt tttttctcta tcagtgctat tagcagggag agatatcacc ggagtttaat
     9361 gtgtgatttt tatttatcgt cgaatctgta ttgtttatca ttgcttttca caacgctaac
     9421 atctaataag attagatatt cacttcgtga gagcttcatg ccttgagagg atctcaattt
     9481 tcttttgcaa tgagacaggc gcttcctgtt gttatggtat agtaccccgt tattgagcct
     9541 cctgaacagt gatgctgaat aacataacca catgatatat cgataaaata atctctacat
     9601 ttgaaaatgc acggtaattc tgaaatgcaa aaaatcaacc aaaccagcgc aatgcctgaa
     9661 aaaactgacg ttcactggag tggtcggttt agcgttgcac caatgctcga taggatgtac
     9721 cgtttttgaa aaacaaatag ttatatactt tgtgggagcc tattgggaac ccggcgtttt
     9781 catttcaagg tgtaatccat acgcggctta agaatgagat ataatgcgac ttttagtgtt
     9841 ccgcttgaga ggccatgatg cttaccctgg acgagatagg tcaatcagta cgtaacaata
     9901 tccagttgat tattgatcat gtcggcttac ctcttgctgt tggtccgctc agtgatgatg
     9961 attacaagat tctgtgtggt ggctatggtg agcttgaatg ggactatgcg ttaagtacct
    10021 atggcaactc cagagaaaag tatgagttct gcataaaact tgttcagcaa ggtcgggttc
    10081 agggaatacc atcaggagca gcaatttgtg tttatggggt tgaagaaaac atctttcgta
    10141 tccatatgat cgaaaggttt tctagagaag atgaatctca cccattgaaa gggcgcatgg
    10201 ttttactcac tcttatgagt gcttttatat tttgtaaagc tgttgaatgt aaagttgtcc
    10261 acattgtaga gccagtacca gaactggtgc agtattacga gtcttttggt ttccgcatgg
    10321 aacagtgcgg ttatgtgatg tcggcagtca ttgatgagct gcaggatatc tttcttaaat
    10381 ttgctcagta ggtatagacg agaagggtct acaaattgta ggatacccgt ccagattacc
    10441 ttaaaggtac atctatggca gtcgttttgt gcttaaacta ctaagaaacg atgtcaccaa
    10501 tcgacatgat cgattggcat aagttagcga aacaagctag ctttaaagaa agggttagag
    10561 acgcctttac tgtctcggga gttttctatg aaagatcaaa aagcaaccaa gccacaggtt
    10621 aagttcgaca caatgaaagc attcgcaggt atgggtgctg ctgttgaagt tctgatgaag
    10681 gctgctccta atgcgttcac tcacgctact gtctctggta aagagcagca gggtaagctt
    10741 cgtcgtcgca aagcagcatg atcatagctg gtgctttttg aaaacccgcc ttcaggcggg
    10801 ttttttcttt agtgatgttc tttgtccttc tgtttgactg ttctgacctg ttcccactcg
    10861 atacgtcctt cttctcgcct tttgtctatg tattccgcaa gatcttgaat attgatgcaa
    10921 cgttttgctt tttgtgatgt gccgatgcga tatgttggaa cgggcaactt acaagcgttt
    10981 gcttttgctt ctgccgtggc tggactcata ccaaagtact tttggctaac tgctgagagt
    11041 tcaatgtttg gggtattgaa ttcagccatc agtaaaaaca aggtgttcat aattttctcc
    11101 atcaaaaccg gctgcacccg ggaaaatcat aattctgtgc tggtggcagg aattaatttc
    11161 tgccagatag cggaaacata ttttgcctga tgacgggcat cagccagggc gttgtgccgt
    11221 tcgccatcga aaggcatgtc cattttgggg tcgaatccga tggaacgccc aagcgtaacg
    11281 atcgtgcgta catcgtggtc attccagtac gcccacgggc agatttgtcc tgctcgctcg
    11341 taagctccac gtaaaattac gttgtcgaag gtggccccgt taccccagac ttttaaatat
    11401 ttcgtattgt ctgcgtgccg gttaatgaaa tggtttagtt ctgagagagc atcgctgatc
    11461 gacaaagtat catcaatact tccagcgata ccagcgcgac tttaaatgcg gtaagtacgt
    11521 ttttaaccac atcgattttg aatactattt catcacatac aaacgattta tcgtctacta
    11581 ccgtttcaat tccggtaatc gtgttttgta gccatttggt taattcagcc atttttcatt
    11641 accgcccttt cgggcggtct cctgatgttc tgagggtgca ggaatccctc cggttaagga
    11701 ttaaatttta tttacagaac tgaatttaat tattcagata tacgtatctg taacct
//
LOCUS       DAZFAB010000128        11543 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZFAB010000128 DAZFAB010000000
VERSION     DAZFAB010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.128"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..144
                     /locus_tag="ACHJWA_004089"
     CDS             <1..144
                     /locus_tag="ACHJWA_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFO4310581.1"
                     /translation="NIKIKNMTLDEINNTYCNNDYYNEAIREEPIDFLDRSFSSSSWP
                     FYH"
     gene            450..1838
                     /gene="narU"
                     /locus_tag="ACHJWA_004090"
     CDS             450..1838
                     /gene="narU"
                     /locus_tag="ACHJWA_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFO4310582.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWKNKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAR
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRMKMKGGSDEQA
                     QREAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1920..5660
                     /gene="narZ"
                     /locus_tag="ACHJWA_004091"
     CDS             1920..5660
                     /gene="narZ"
                     /locus_tag="ACHJWA_004091"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFO4310583.1"
                     /translation="MSKLLDRFRYFKQKGDTFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKSLSYKQVRGRGGFIR
                     SNWPELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGKETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLAGGSTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLSPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRICPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5657..7201
                     /gene="narH"
                     /locus_tag="ACHJWA_004092"
     CDS             5657..7201
                     /gene="narH"
                     /locus_tag="ACHJWA_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO4310584.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIDAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSIAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7201..7896
                     /gene="narW"
                     /locus_tag="ACHJWA_004093"
     CDS             7201..7896
                     /gene="narW"
                     /locus_tag="ACHJWA_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO4310585.1"
                     /translation="MQILKVIGLLMEYPDEMLWECKEDALALIRRDAPMLTDFTRNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     TLSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7893..8573
                     /gene="narI"
                     /locus_tag="ACHJWA_004094"
     CDS             7893..8573
                     /gene="narI"
                     /locus_tag="ACHJWA_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO4310586.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8652..9545
                     /gene="yddE"
                     /locus_tag="ACHJWA_004095"
     CDS             8652..9545
                     /gene="yddE"
                     /locus_tag="ACHJWA_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFO4310587.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTVWQTSLAGK
                     HRVTIEKHHDGYRISLEQGTPGFEPPLEGETRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLRVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGAAVILFHAEWAIKL"
     gene            complement(9641..10485)
                     /gene="nhoA"
                     /locus_tag="ACHJWA_004096"
                     /pseudo
     CDS             complement(9641..10485)
                     /gene="nhoA"
                     /locus_tag="ACHJWA_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
     gene            10784..11227
                     /locus_tag="ACHJWA_004097"
     CDS             10784..11227
                     /locus_tag="ACHJWA_004097"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFO4310588.1"
                     /translation="MPVEFEPPRVTIVVDKSTWTRELIERNGKFGIVIPGVAATNWTW
                     AVGSVSGREEDKFNCYGIPVVRGPVFGLPLVEEKCLAWMECRLLPATSAQEEYDTLFG
                     EVVSAAADARVFVEGRWQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11231..11458)
                     /gene="pptA"
                     /locus_tag="ACHJWA_004098"
     CDS             complement(11231..11458)
                     /gene="pptA"
                     /locus_tag="ACHJWA_004098"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HFO4310589.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIAPQMEALIKKPGYSM"
BASE COUNT         2743 a         2981 c         3026 g         2793 t
ORIGIN      
        1 aatataaaaa ttaaaaatat gaccttagat gaaataaaca atacttattg taataacgat
       61 tattacaatg aggcaataag agaggaaccg atagactttc tggacagatc gttttcctcc
      121 agctcatggc ctttttatca ctaattctat aagtgtctgg tcttgttaaa aatatagtta
      181 atatacaacc ctttggattc atcatctgaa gggttgaatc atgacatgcg tacatagctt
      241 aaccccgttt aatggttagc ttgttagtta catttagtaa cacataactg gctgcttaat
      301 ccccattctg cccccgcggt tacgctttct tatctattct ttcaatcagc acttcaccca
      361 tttcatcctt aattctaata actggctatt gcgccttacc tcctgttttg tctttttttc
      421 attaccaatg tgtacatgtg aggaacaata tggcactgca aaatgagaaa aatagtcgtt
      481 atcttttacg cgactggaaa ccagaaaatc cggccttctg gaaaaataaa ggaaaacata
      541 ttgctcgaag aaacctctgg atatcagtca gttgtctact tcttgccttc tgtgtctgga
      601 tgctatttag cgcagttacc gttaatctca ataaaatcgg ttttaatttc actaccgatc
      661 aactcttttt attaaccgca ttaccctccg tttctggcgc attattacgt gtcccctact
      721 cctttatggt gcctatattc ggtggacgtc gatggacggt ttttagtact gcaatcctga
      781 ttattccttg cgtctggctc ggaattgccg tgcagaatcc gaatactcct tttgggatat
      841 ttatcgttat cgctttgcta tgcggttttg caggtgcgaa ctttgcttcg agcatgggca
      901 atatcagttt cttctttcca aaagccaggc aaggtagcgc tcttgggatt aatggcggat
      961 taggaaactt aggtgtaagt gtaatgcagc tggttgcacc gctggtcatt tttgtacccg
     1021 tatttgcctt tctcggcgtc aatggagtac cgcaggccga cggttcggtg atgtcgctgg
     1081 cgaatgccgc atggatttgg gtaccgctac tagcgattgc cacgatcgcc gcctggtcag
     1141 ggatgaatga tatcgccagt tcacgcgcgt caattgccga ccagctccct gtcttacaac
     1201 gcctgcatct ctggctgctg agcctgcttt accttgccac cttcggttcg tttatcggtt
     1261 tttctgcggg tttcgccatg ctggcaaaaa cccagttccc ggatgtgaat attctgcgcc
     1321 tggcgttctt tggcccattt atcggtgcca tcgcgcggtc ggtgggtggt gctatttccg
     1381 ataaattcgg cggcgtgcgg gtgacgttga tcaactttat ttttatggcg attttcagcg
     1441 ccctgctgtt ccttacctta ccgggcacag gctccggtaa cttcatcgcc ttttacgccg
     1501 tatttatggg gctgtttctg accgcgggtc tgggaagtgg ttctactttc cagatgatcg
     1561 ccgtcatctt tcgccagata accatttatc ggatgaagat gaaaggcggt agtgatgagc
     1621 aagctcagag agaagccgtc accgaaacgg cggcggctct gggctttatc tcagccatcg
     1681 gcgcagtggg cggctttttt attccgcagg catttggcat gtcactcaat atgaccggct
     1741 ctccagtggg cgcgatgaaa gtgtttttaa tcttctacat cgtttgtgtg ctactgacct
     1801 ggctggttta tggtcggcgg aagttcagcc aaaaataaga gatcgatgcc tgatgtacaa
     1861 ccgtgtcgca tccgacaagg catcagatcc caattcattc ctggagcagg agtcatgtca
     1921 tgagtaaact tttggatcgc tttcgctact tcaaacaaaa gggcgatacc tttgccgatg
     1981 gtcacggaca ggtgatgcat agcaaccgcg actgggagga cagctatcgc cagcgttggc
     2041 agttcgacaa aatcgtgcgt tccacccacg gtgttaactg tacaggctcc tgtagctgga
     2101 aaatctacgt taaaaatggt ctggtgacct gggaaatcca acagaccgac tacccgcgca
     2161 ctcgccctga cctgcccaat catgaacctc gcggctgccc gcgtggcgca agttactcct
     2221 ggtatcttta cagcgctaac cgcctgaaat acccgctcat tcgtaaacga ctgattgaac
     2281 tgtggcgcga agccctcaaa caacacagcg atccggtact ggcgtgggca tcgattatga
     2341 acgatccgca aaagagcctg agctacaaac aagtgcgtgg gcgcggcggg tttatccgct
     2401 ccaactggcc ggaactaaac cagctgattg ccgccgctaa cgtctggact atcaaaacct
     2461 acggcccgga tcgcgttgcc ggtttctcgc cgatcccggc gatgtcgatg gtttcttacg
     2521 ccgccggaac acgttatctg tcactattag gtggcacctg tttaagcttc tacgactggt
     2581 attgcgacct gccgcccgcc tcgccgatga cctggggcga gcaaaccgac gtaccggaat
     2641 ctgccgactg gtataactcc agttacatca tcgcgtgggg gtctaacgta ccgcagacac
     2701 gtacgccgga cgcccatttc tttaccgaag tacgctacaa aggcactaaa accatcgcca
     2761 ttacccctga ctactctgaa gtggccaaat tgtgcgacca gtggctggca ccaaaacaag
     2821 gcactgatag cgccctggcg atggcaatgg gccatgtgat tttaaaagag tttcatctcg
     2881 ataatcccag cgactacttt atcaactact gccgccgcta tagcgacatg ccgatgctgg
     2941 taatgctgga acctcgcgac gatggtagct acgtacccgg gcgcatgatc cgcgcgtctg
     3001 acctggtaga tggactaggc gaaagcaata atccgcagtg gaaaaccgtg gcggttaata
     3061 ccgcaggtga attggtagtg ccgaatggtt cgattggttt ccgctgggga gaaaaaggca
     3121 aatggaatct ggaatccatt gccgccggta aggaaaccga attgtcgtta accctgctcg
     3181 gtcaacatga cgccgttgca ggcgtggctt tcccctactt tggcggcatc gaaaatccgc
     3241 attttcgcag cgtcaaacac aatcctgtcc tggtgcgtca attacccgtt aaaaacctga
     3301 cattagccgg tggcagcacc tgtccagtgg tcagcgttta tgatttggta ctggcgaatt
     3361 acggcctcga tcgcgggctg gaagatgaaa acagcgcgaa agattacgct gaaatcaaac
     3421 cgtacacccc agcctggggt gagcaaatta ccggcgtgcc gcgccagtat attgaaacca
     3481 tcgctcgtga atttgccgat actgcccata aaacgcatgg gcgctcgatg attatcctcg
     3541 gcgcaggtgt taaccactgg tatcacatgg acatgaacta ccggggaatg atcaatatgc
     3601 tgatcttctg cggttgtgtc ggacaaagcg gtggcggctg ggcacactat gtcggtcagg
     3661 aaaaactgcg cccgcaaacc ggctggttgc cgctggcctt tgcgctcgac tggaaccgac
     3721 caccgcgcca gatgaacagc acctcgtttt tctacaatca ttccagccaa tggcgctatg
     3781 aaaaagtctc tgcgcaggag ttactttcac cgcttgccga tgccagtaag tacagcggtc
     3841 atctgattga ttttaacgtt cgcgccgaac gcatgggctg gctaccttct gcgccgcagt
     3901 tggggcgtaa cccgctcggg attaaagctg aagccgacaa ggccggatta tcccccacag
     3961 aatttaccgc ccaggcgctg aaatcgggcg atttacgtat ggcctgcgaa caaccagata
     4021 gcggcagcaa tcatccgcgt aatttgtttg tctggcgttc taacctgctt ggctcctccg
     4081 gcaaaggcca cgagtatatg cagaagtatc tgctggggac cgaaagcggg attcagggcg
     4141 aggaactcgg tgccagcgac gggatcaaac cggaagaagt cgagtggcaa actgcggcga
     4201 ttgaaggcaa gctcgacctg ctggtgacgc tcgacttccg catgtccagt acctgcctgt
     4261 tctccgatat cgttctgccc accgccacct ggtatgaaaa agacgatatg aacacctcgg
     4321 atatgcatcc atttattcat ccgctttctg cggcggtcga tccggcctgg gaatcacgca
     4381 gcgactggga aatctacaaa ggtattgcca aagcattttc gcaagtgtgc gtgggccatc
     4441 ttggcaaaga aaccgacgtg gtattacaac cactgctgca tgactctccg gcagagctct
     4501 cacagccgtg tgaagtgctc gactggcgca aaggcgaatg cgatctgagc ccgggtaaaa
     4561 ccgcgccgaa tattgtggcg gtggagcgcg actaccctgc tacgtatgaa cgctttacct
     4621 cgctcgggcc attgatggac aaacttggca acggcggtaa agggatttcg tggaatacgc
     4681 aggatgaaat cgatttcctc ggtaaactca attacaccaa gcgtgatggc ccagcgcagg
     4741 ggcgtccgct gattgacacc gccattgacg cttcagaagt gattctggca ctggcaccag
     4801 aaaccaacgg tcatgttgca gttaaagcgt ggcaggcgct gggcgagatc accggacgcg
     4861 aacataccca tctggcgctg cacaaagagg acgagaagat tcgctttcgc gatattcagg
     4921 cgcagccgcg taaaattatc tccagcccca catggtctgg tctggaaagc gatcacgtct
     4981 cctataatgc gggatacacc aacgttcatg agttaattcc gtggcgcacg ctgtcgggac
     5041 gccagcagct ctatcaggat catccgtgga tgcgtgcttt tggtgaaagc ctggtggcat
     5101 atcgcccgcc tatcgacacc cgtagcgtca gtgagatgcg ccagatcccg ccaaacggct
     5161 tcccggaaaa agcacttaac ttcctgacgc cgcaccagaa atggggcatt cactcaacct
     5221 acagtgaaaa cctgctaatg ctgacgctct ctcgcggtgg accgattgtc tggatcagcg
     5281 aaaccgatgc ccgtgaacta accattgtcg ataacgactg ggtggaagtg tttaacgcca
     5341 atggcgcgct gacggcccgc gcggtggtca gccaacgtgt accgccgggt atgaccatga
     5401 tgtatcacgc tcaggaacgc attatgaata ttcctggttc ggaagtaact ggcatgcgcg
     5461 gcggtattca taactcggtc acccgcattt gcccgaaacc aacgcatatg attggcggtt
     5521 acgcgcagct ggcctggggc tttaactact acggcaccgt cggctcgaac cgcgacgaat
     5581 tcatcatgat ccgcaagatg aagaacgtta actggctgga tgatgaaggt cgcgatcagg
     5641 tacaggaggc gaaaaaatga aaatccgttc gcaagtcggc atggtgctta atctcgataa
     5701 atgtatcggc tgccatacct gttcggtgac ctgtaaaaac gtctggaccg gacgcgaagg
     5761 catggagtac gcatggttta acaacgtcga aaccaaaccg ggcattggtt atccgaaaaa
     5821 ctgggaagat caggaagagt ggcaaggcgg ctgggtccgc gatgtgaatg gcaagatacg
     5881 cccgcgtctg ggcagcaaga tgggtgtaat aaccaaaatc ttcgccaacc cggtggtgcc
     5941 gcagattgat gattactacg aacctttcac cttcgactac gaacatttgc atagcgcacc
     6001 ggaaggcaaa catatcccta ctgctcgccc gcgttcgctg attgacggta agcggatgga
     6061 caaagtgatc tgggggccaa actgggaaga actgctgggc ggcgagtttg aaaaacgtgc
     6121 ccgcgaccgc aacttcgagg ccatgcaaaa ggagatgtac gggcagtttg aaaacacctt
     6181 catgatgtac ctgccgcgcc tgtgcgaaca ctgcctcaat cccagctgcg tggcgacctg
     6241 cccaagcggc gctatctaca aacgcgaaga agacggcatt gtgctgattg atcaggataa
     6301 atgccgtggc tggcgtttgt gcattagcgg ttgtccgtac aaaaaaatct acttcaactg
     6361 gaaaagcggc aagtcagaaa aatgtatctt ctgttaccca cgaattgagt ccggtcaacc
     6421 gaccgtgtgc tcagaaacct gcgtgggtcg catccgatat ctgggcgtgc tgctttacga
     6481 cgctgaccgc atcgaggaag cggcgagcac cgagcgcgag gttgacctct atgaacgcca
     6541 gtgcgaagtg ttcctcgatc cgcacgatcc ctcagtgatc gaggaagccc tgaaacaagg
     6601 tattccacaa aatgtgattg acgctgccca gcgttcgcca gtctacaaaa tggcgatgga
     6661 ctggaaactg gcactaccgc tgcaccctga ataccgcacc ctgccgatgg tctggtacgt
     6721 tcctccgctg tcaccgattc agtcctacgc agatgcgggc ggtttgccga aaagcgaagg
     6781 cgtgctgccc gccatcgaaa gcctgcgtat tccggtgcaa tatctcgcca atatgttgag
     6841 tgccggcgat accggtccgg tactgcgggc gctgaaacgg atgatggcga tgcgccacta
     6901 tatgcgttca caaaccgtgg aaggcgttac tgatactcgt gccatcgacg aagtaggcct
     6961 gagcatcgcc caggtcgaag aaatgtatcg ctatctcgcc attgccaact atgaagatcg
     7021 ttttgttatc ccgaccagcc atcgggaaat ggcgggcgat gccttcgcag aacgcaatgg
     7081 ctgcggtttt acctttggtg acggttgtca cggctcggac agcaaattca acctgttcaa
     7141 cagtagccgt atcgatgcca tcaacatcac cgaagtgcgc gacaaagcgg agggcgaata
     7201 atgcagatcc tcaaagtgat cggcctgttg atggagtatc cggacgagat gttgtgggaa
     7261 tgcaaggagg acgcgctggc gttgatccgc cgcgatgcgc cgatgcttac ggatttcacc
     7321 cgcaacctgc ttaacgcgcc gctgctggat aaacaggccg aatggtgcga agtgtttgac
     7381 cgcgggcgca ccacgtcgct gctgctgttc gaacatgttc atgccgagtc ccgcgatcgc
     7441 ggccaggcaa tggtggacct gctggcggag tatgaaaagg tcggcctgca gctggattgt
     7501 cgggaactgc ccgattatct accgctgtat ctggagtatt taagcgtact gcctgacgat
     7561 caggcgaaag aaggattgct caacgttgcg ccgatcctcg ccctgcttgg cggtcgctta
     7621 aaacaacgcg aggcaccgtg gtacgcgttg tttgatgctc tgctgcaact ggcgggaagt
     7681 acactttcaa gtgacagtgt cacaaaacaa gtaaacagcg aagagcgcga tgacacccgc
     7741 caggcgcttg atgcagtgtg ggaagaggaa caggtgaagt ttattgaaga taacgccacg
     7801 gcgtgtgaca gctcgccgct taatcaatat cagcgacgct ttagtcagga tgtcgcgccg
     7861 caatatgtcg acatcagtgc gggaggtggg aaatgattca gtatctgaac gtcttttttt
     7921 acgatatcta cccgtacatt tgtgcgacgg tgtttttcct cggcagctgg ctgcgctacg
     7981 actacgggca gtacacctgg cgcgcctcct caagtcagat gctcgataaa cgcgggatgg
     8041 tgatatggtc gaatctgttc catatcggca ttttggggat cttcttcggg cacctgttcg
     8101 gcatgttaac gccacactgg atgtacgcgt ggtttttgcc agtggcagcg aaacaactaa
     8161 tggcgatggt gctcggtggt atttgcggtg tgttgacgtt gattggcggc gcaggactgc
     8221 tgtggcgcag gctgacaaat cagcgcgtgc gtgccacttc cactacgccg gatatcatca
     8281 tcatgagcat tttgctgatc cagtgtctgt tggggctaag taccataccg ttctcagcac
     8341 agtatcctga tggtagcgaa atgatgaagc tggtgggttg ggcgcaaagc attgtcactt
     8401 tccgtggtgg atcgtcagag atgcttaacg gtgtagcgtt cgtcttccgc ctgcatctgg
     8461 tgttgggaat gaccatcttc ttgctcttcc cattcacccg actggtgcat gtgtggagcg
     8521 ccccgtttga gtactttact cgtcggtatc agattgtgcg ctcgcggcga taatttcacc
     8581 ctggccggat agcccttcgc tctccggcca cttgcaggct atgatagttc tccagtcaca
     8641 ggagaacgtt aatgaaaccg caggtttacc acgtcgatgc ttttacctct caaccatttc
     8701 gcggcaattc tgccggtgtt gttttccccg ccgataatct tagcgaagcg caaatgcagc
     8761 ttatcgcccg cgaattaggt cattcggaaa ccgcttttct gctgcacagc gacgacagcg
     8821 atgtgcgcat ccgctacttt acgccaacag ttgaagtgcc tatttgcggt cacgcgacag
     8881 tagctgcgca ctatgtacgt gcgaaggtgt taggtttagg aaattgcacg gtctggcaaa
     8941 catcgctggc aggaaaacat cgcgtgacta tcgaaaagca ccacgatggt tatcgtattt
     9001 cgctggaaca aggtacgcca ggctttgagc caccgctgga aggtgaaaca cgtgcggcga
     9061 ttatcaacgc gcttcatctc actgaggacg atattctgcc aggcttgcca attcaggtgg
     9121 caaccacagg acactcaaaa gtgatgatcc cactgaaacc ggaagtggat atcgacgccc
     9181 tttcgcctga ccttaatgcg ctgaccgcta tcagcaaaca gattggctgc aatggtttct
     9241 tcccgttcca gatccgccca ggcaaaaacg aaaccgatgg tcgcatgttc tcgcctgcga
     9301 ttggtattgt ggaagatccg gtgactggca atgccaatgg cccgatgggt gcatggttgg
     9361 tacatcacaa cgtattgccc cacgatggca acgtgttgcg tgttaaaggc catcaggggc
     9421 gcgcattggg gcgtgacggt atgattgaag tgacggtgac aattcgtgat aaccaaccgg
     9481 aaaaagtcac catttctggc gcggcggtga ttttgtttca tgccgaatgg gcaattaaac
     9541 tctgaaaccg ttgcctgatg cgcttcgcct gtcaggtcta caaccgggct aatgtatgcc
     9601 ggataaggcg tttacgccgc atccggcaac ctgacataaa ttattttccc gcctccgggt
     9661 gcgtatcaaa cgccgccatc accagcgcta actcatccac ggtaaagcca tgtttcgcat
     9721 catccacgcc cagaccaaac tgttcttgca tcacagcata taacgacgcc acatccggta
     9781 gatttcgctg ctccaccgcg tgcccatttt cataatggga aaatgaaaat tggtcagtgt
     9841 cagcttgccg ccgtccggca aatggcggca catcagcaaa tgatggcgaa aatgcgactg
     9901 cggccagtgc gccgaccaga aattacccat cacataatcg ctttgttgct gctcgcagag
     9961 atcaaaacgg tacatcgact gccagtgctg atgatgatta aactgcaaca cccaatcatc
    10021 accctcctgc aacaaccgat actctccgtg tggcgtggtc tgcacaagat cgggaactaa
    10081 acgaatcggc gcggttagcg tctgcccacc gaaaccgaca tcagcaatcc atttttcctc
    10141 ttccagttcc accaacagca aacgatgggt gcgcggcggt aatgctggcg gatttgataa
    10201 cactacccgc cctaacaagc tgcgaacgtt aaaccccagc tcgcgtaaca cccgctcaaa
    10261 aacgccattc tgctcaaaac agtaaccgcc acgacgggct atcaccagtt tctcttccag
    10321 cgattgatca tcaagctgca tttccctcgg cagcaaaacg tcgaggtttt caaacggaat
    10381 ggtgcaattg tgtttcaggt gcaatgcacg aagcgtatca atattgaccg cagcagctcc
    10441 cgaccagtta atacgggcaa aatagtgatt cagaatgggc gtcatcaggc gtttccctta
    10501 gtggttttct caacttataa cctggaatag acgccgcacc ggctattttg tgcgatcgcg
    10561 gacatgcatc acgttcgcaa atcgccaatc ccgttaattt cgttacttat tgcgttacgg
    10621 actacgctta aaaaaacctt cttcgcgagg cgataccatg agccgattca tccccataga
    10681 attacaccat gccagccggc tgttaaatca tggcccaact gtcatgatca ccagtttcga
    10741 tgagcaatcc cagcggcgta acattatggc tgcagcctga tcaatgccgg tggagtttga
    10801 acctccacgc gtgacgattg tggtagataa atcgacatgg accagggagt tgattgaacg
    10861 taacggtaaa tttggcatcg ttatcccggg cgttgcagca actaactgga cgtgggcagt
    10921 gggaagtgtg tcgggtcgtg aagaagataa atttaattgc tatggcattc cggttgtgag
    10981 aggcccggta tttggtttgc ctctggtcga ggaaaaatgt ctggcgtgga tggagtgtcg
    11041 attgctacct gcgacttctg cgcaagaaga atacgacacg ctgtttggcg aagtagtatc
    11101 agcagcggca gacgcacggg tatttgtcga aggccgctgg cagtttgatg atgataagct
    11161 caatacgttg catcatttag gtgctgggac gtttgttacc agcggcaagc gtgttacggc
    11221 gggttaagca ttacatgctg taaccgggtt tctttatcaa agcctccatt tggggcgcga
    11281 tttcggcatc ccagatagct tgccaagatt ctggttgaat ctgttgtaga gcaatgctta
    11341 tcgaactgtc tttactgttc agatggcgaa taataacgtc ggtaatatct gcagcaagtg
    11401 ctgctttttg ttgttcgtcc agttcacgcg gaaaacattt aatgtctatg tgcggcatga
    11461 ttacgctcct tttgaaagat gtcagtaaaa ttaacacaca ttgtgaatgt cgtgtgtccg
    11521 gtaaagcgtt tttacgacaa taa
//
LOCUS       DAZFAB010000129        11394 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZFAB010000129 DAZFAB010000000
VERSION     DAZFAB010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.129"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            138..4040
                     /gene="espP"
                     /locus_tag="ACHJWA_004099"
     CDS             138..4040
                     /gene="espP"
                     /locus_tag="ACHJWA_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter EspP"
                     /protein_id="HFO4310590.1"
                     /translation="MNKIYSLKYSHITGGLIAVSELSGRVSSRATGKKKHKRILALCF
                     LGLLQSSYSFASQMDISNFYIRDYMDFAQNKGIFQAGATNIEIVKKDGSTLKLPEVPF
                     PDFSPVANKGSTTSIGGAYSITATHNTKNHHSVATQNWGNSTYKQTDWNTSHPDFAVS
                     RLDKFVVETRGATEGADISLSKQQALERYGVNYKGEKKLIAFRAGSGVVSVKKNGRIT
                     PFNEVSYKPEMLNGSFVHIDDWSGWLILTNNQFDEFNNIASQGDSGSALFVYDNQKKK
                     WVVAGTVWGIYNYANGKNHAAYSKWNQTTIDNLKNKYSYNVDMSGAQVATIENGKLTG
                     TGSDTTDIKNKDLIFTGGGDILLKSSFDNGAGGLVFNDKKTYRVNGDDFTFKGAGVDT
                     RNGSTVEWNIRYDNKDNLHKIGDGTLDVRKTQNTNLKTGEGLVILGAEKTFNNIYITS
                     GDGTVRLNAENALSGGEYNGIFFAKNGGTLDLNGYNQSFNKIAATDSGAVITNTSTKK
                     SILSLNNTADYIYHGNINGNLDVLQHHETKKENRRLILDGGVDTTNDISLRNTQLSMQ
                     GHATEHAIYRDGAFSCSLPAPMRFLCGSDYVAGMQNTEADAVKQNGNAYKTNNAVSDL
                     SQPDWETGTFRFGTLHLENSDFSVGRNANVIGDIQASKSNITIGDTTAYIDLHAGKNI
                     TGDGFGFRQNIVRGNSQGETLFTGGITAEDSTIVIKDKAKALFSNYVYLLNTKATIEN
                     GADVTTQSGMFSTSDISISGNLSMTGNPDKDNKFEPSIYLNDASYLLTDDSARLVAKN
                     KASVVGDIHSTKSASIMFGHDESDLSQLSDRTSKGLALGLLGGFDVSYRGSVNAPSAS
                     ATMNNTWWQLTGDSALKTLKSTNSMVYFTDSANNKKFHTLTVDELATSNSAYAMRTNL
                     SESDKLEVKKHLSGENNILLVDFLQKPTPEKQLNIELVSAPKDTNENVFKASKQTIGF
                     SDVTPVITTRETDDKITWSLTGYNTVANKEATRNAAALFSVDYKAFLNEVNNLNKRMG
                     DLRDINGEAGAWARIMSGTGSASGGFSDNYTHVQVGVDKKHELDGLDLFTGFTVTHTD
                     SSASADVFSGKTKSVGAGLYASAMFDSGAYIDLIGKYVHHDNEYTATFAGLGTRDYST
                     HSWYAGAEAGYRYHVTEDAWIEPQAELVYGSVSGKQFAWKDQGMHLSMKDKDYNPLIG
                     RTGVDVGKSFSGKDWKVTARAGLGYQFDLLANGETVLRDASGEKRIKGEKDSRMLMSV
                     GLNAEIRDNVRFGLEFEKSAFGKYNVDNAVNANFRYSF"
     gene            complement(4296..4777)
                     /locus_tag="ACHJWA_004100"
                     /pseudo
     CDS             complement(4296..4777)
                     /locus_tag="ACHJWA_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            5482..5616
                     /locus_tag="ACHJWA_004101"
     CDS             5482..5616
                     /locus_tag="ACHJWA_004101"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310591.1"
                     /translation="MPHPERNHCLLLMGYLHGVQASELLGIKLSDIDLQAGNLNIRRL
                     "
     gene            6218..7039
                     /locus_tag="ACHJWA_004102"
     CDS             6218..7039
                     /locus_tag="ACHJWA_004102"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFO4310592.1"
                     /translation="MLNARHLPVLMYHHVSRCPGLVTLSPETFCEQMKWLADNNWRTV
                     TSAELEYFYQGGTLPRKSVMLTFDDGYLDNWLRAWPVLKEYNLHAHIFLITGLIGKGN
                     VRSRQEQEYSHRDCERLIADNRSDEVMLRWSEVREMRDSGLVEFHVHTHSHKRWDRLS
                     VSRAEQCRLMKEDILVGKQCLTEKLGFCSSHLCWPEGYYNRDYINLAGKLGFSYLYTT
                     ERRMNCPENGSLRIGRISTKEREHSGWLKRRLFYYTTPLFSSVLALHKGPRLPDN"
     gene            7039..8145
                     /locus_tag="ACHJWA_004103"
     CDS             7039..8145
                     /locus_tag="ACHJWA_004103"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO4310593.1"
                     /translation="MMKILFTESSSDIGGQELQALAQMTALQKQGHSVLLACREKSKI
                     APEARKRGHDVTFIPFRNSLHLPSILRLRRIIGEFKPDLVICHSGHDSNIAGLSRLIC
                     CHRFSIVRQKTYITRKTRTFSLNYLCDFIVVPSSAMMAHLMAEGVRTPVTVIPPGFDW
                     PALHNEAMRPLPLHIHAWAASADNVPLIVQVGMLRPEKGHEFMLRVLYQLKMEGKSFR
                     WLVVGAGREEYEARLRQQTEHLGMSGDVLMAGALFPALPVYRIASVVVMPSENEAFGM
                     VLAEASVSGVPVIASETGGIPDVIQKNVTGTLLPVGDVSAWTGALRDFLSRPERFRMM
                     AASAREDIEYRFDINRTAQIIVSLASQAKGKCNR"
     gene            8235..9956
                     /gene="cptA"
                     /locus_tag="ACHJWA_004104"
     CDS             8235..9956
                     /gene="cptA"
                     /locus_tag="ACHJWA_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFO4310594.1"
                     /translation="MHLNTGQNRPTFSWSALGWAIFYFGFFSTLLQVIIFSSGYSGTN
                     GIRDSLLFSCLWLIPVFLYPDRIKIIAAVVGFILWGTSLAALCYYFLYGHEFSQSVLF
                     VMFETNAREAGEYFSQYFSLKLLLISLVYTAVSVFLWTRLRPVYIPLPWRRIVSFLLL
                     YALLLHPVVLKSLIRQEPLNDTLGKLASRMEPAAPWQFVSSYYQYHQQLNALTTFLNE
                     NSALPPLGNLRDESGERPRTLVLVIGESTQRERMSLYGYLRETTPELDALRKTDPGLT
                     VFNNVVASRPYTIEALQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTIT
                     ARNTMLTVFSRQTDRQYYMNQQRTQSAREYDTNVLKPFREVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPEGQGRFDGITGHIPTGLNAKELEVYNDYDNANLFNDHVVASLIKDFRAT
                     APDGFLLYFSDHGEEVYDTPPYKTQGRNEDNPTRPMYTVPFLLWTSEKWHAAHPRDFS
                     QYVDRKYSLAELIHTWSDLAGLTYDGYDPTRSLVNPQFRETTRWIGNPYKKNGLTDFD
                     TLPYGEP"
     gene            10030..11028
                     /gene="lpxM"
                     /locus_tag="ACHJWA_004105"
                     /gene_synonym="msbB"
     CDS             10030..11028
                     /gene="lpxM"
                     /locus_tag="ACHJWA_004105"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052689.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFO4310595.1"
                     /translation="MVPPSAVLCYHNEISRQIPVNMKNIRTEFIPRFNLTLCFPRYWM
                     TWTGIGIICVFAMVPPALRDPLLGKLGMLVGRLGKSARQRALINLSLCFPEYSDKEKE
                     NIVDAMFATASMAVVLMAELALSGPDKISHRIRWNGLEIVEKMAQNNEKVIFLVPHAW
                     GVDIPAMLMAASGRKMAAMFHNQRNPVVDYVWNSVRRRFGGKLHARNDGIASFVRSVR
                     QGYWGYYLPDQDHGPEFSEFADFFATYKATLPVIGRLSRISGARIIPLFPVYDGKTHH
                     LTIHVSPPLAIRQKSDAHIARQINEVVENFVRPHPEQYTWILKLLKTRKEGEEDPY"
     gene            11081..11269
                     /locus_tag="ACHJWA_004106"
                     /pseudo
     CDS             11081..11269
                     /locus_tag="ACHJWA_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767718.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3166 a         2471 c         2719 g         3038 t
ORIGIN      
        1 tgtttgttat ccgtatgata acaaacacat aaaggagtaa acatttatct ggacgatgat
       61 tacaatgttc acgactgtca gagacacacc tctgatacat tagaaaaacc aatgtttccc
      121 ttaaaaatgg agcttatatg aataaaatat actctcttaa atacagccat attacaggag
      181 ggttaatcgc tgtttctgaa ttatccggca gagtatcatc aagagcaact ggtaagaaaa
      241 aacacaaacg catacttgca ttatgttttt taggcttatt acaatcctca tattcttttg
      301 cgtcacagat ggatatttca aatttctaca tccgtgacta tatggatttt gcacagaaca
      361 agggcatatt tcaggctggc gcaacaaata ttgaaatagt gaagaaagat ggctccaccc
      421 tgaaactacc ggaagtacca tttcctgact tctcaccggt tgcaaacaaa gggtcaacca
      481 catctattgg tggtgcatac agtatcacag ccacacacaa tacgaaaaac caccactcag
      541 ttgcgacgca aaactggggg aacagcacgt acaaacaaac tgactggaat acttcacatc
      601 ctgattttgc agtatcccga cttgacaagt ttgttgttga gacccgaggt gcgactgaag
      661 gcgcagatat ttcgttatca aaacagcagg cacttgaacg ttacggggtt aattataaag
      721 gagaaaagaa acttatcgca ttcagagccg gctctggtgt ggtatccgtt aaaaaaaatg
      781 gacgcataac tccatttaat gaggtttctt ataagccaga aatgttaaat ggctctttcg
      841 ttcacattga tgactggagt ggatggctga tattaaccaa caaccagttt gatgagttta
      901 ataacattgc ctctcagggt gacagcggtt cagcactgtt cgtctatgat aaccaaaaga
      961 aaaagtgggt tgtcgctgga actgtctggg ggatttataa ttacgccaat ggcaaaaacc
     1021 acgcagcata cagtaaatgg aaccagacaa ccattgacaa cctgaagaac aagtattctt
     1081 acaacgtgga tatgtcaggg gctcaggttg caaccattga aaatggaaaa ctgacaggca
     1141 ctggctcaga caccaccgat ataaaaaata aggacttaat atttactggc ggtggagata
     1201 tcctcctgaa atcctctttt gataatggtg ctggcggtct tgtctttaat gataaaaaga
     1261 cctatcgagt aaacggggat gatttcacct ttaaaggtgc cggtgttgat acaagaaacg
     1321 gcagcaccgt tgagtggaat atccggtatg ataataaaga caaccttcac aaaattggtg
     1381 atggcacatt agatgtccga aaaacccaga acaccaacct gaaaacaggt gagggtcttg
     1441 tcattcttgg agctgaaaaa acattcaata atatctacat aaccagtggt gatggaactg
     1501 tccgactgaa tgcagaaaat gcactgtctg gcggtgaata caacggtatt ttctttgcga
     1561 aaaatggcgg aactcttgac ctgaacggat ataatcagtc tttcaataaa attgctgcaa
     1621 ctgattcagg tgctgtaata accaatacgt caaccaaaaa atccatttta tccctgaata
     1681 atactgctga ctatatctat cacggcaaca taaacgggaa tctggacgta cttcagcatc
     1741 atgagacgaa aaaagagaac cgtcgtctta ttcttgatgg gggcgtggac acaacaaatg
     1801 atataagcct gcgtaataca caactgtcca tgcagggaca tgccactgaa catgccattt
     1861 atcgggatgg agctttctct tgttcactac cagctcctat gcgctttttg tgtggcagtg
     1921 attatgttgc aggaatgcaa aatacagaag ctgatgctgt aaaacaaaac ggaaatgcct
     1981 ataaaaccaa caatgctgtc tctgatttat cgcagccaga ctgggaaacc ggaacattca
     2041 gatttggaac gctacatctt gaaaattccg atttttctgt tggtcgtaat gcaaatgtaa
     2101 tcggggacat tcaggccagt aaatcaaaca ttactattgg tgacactaca gcatatattg
     2161 atttgcatgc tggtaaaaat attaccggtg atggttttgg cttccgccag aatattgtgc
     2221 gtggaaactc acaaggagaa acgctgttta caggagggat cacagcagaa gacagcacta
     2281 tcgttattaa agataaagca aaagcattat tttcaaatta tgtatacctg ctgaacacaa
     2341 aagcaaccat agagaacggt gctgatgtga caactcaaag tggtatgttc tccacgagcg
     2401 atatcagcat ctctggtaat ctgtccatga caggcaatcc cgacaaagac aataaattcg
     2461 agccctcaat atatctgaat gatgcttctt atctactgac tgacgactcc gccagactcg
     2521 ttgccaaaaa taaagcatct gtggtgggag atatacactc cactaaaagt gcatccatca
     2581 tgtttggtca tgatgaaagc gacctctcgc agttgtctga cagaacctca aaagggcttg
     2641 cacttggtct tttaggtggc tttgatgtct catatcgcgg ttcagtcaat gccccgtcag
     2701 catctgccac tatgaacaac acctggtggc aactaaccgg agattctgcg ctgaaaacac
     2761 tgaaaagtac aaacagcatg gtctatttca ctgacagcgc aaacaataag aaattccata
     2821 cgctgacggt cgatgagctg gcaaccagca acagcgccta tgcgatgcgt acaaaccttt
     2881 ctgaatcaga caaactggag gtcaaaaaac acttgtctgg tgagaacaat attttactcg
     2941 ttgatttcct tcagaaacca acgcctgaaa aacaactgaa tattgaactg gtaagcgcgc
     3001 caaaagacac caatgaaaat gtctttaaag ccagtaaaca aaccattggt ttcagtgatg
     3061 taacgccggt cattacaacc agggaaaccg atgacaaaat aacatggtca ctgacaggct
     3121 ataacacggt agcaaacaag gaagcaaccc ggaatgccgc cgccctgttc tctgttgact
     3181 ataaagcgtt tctgaacgag gtcaacaacc tgaacaaacg tatgggtgac ctgcgtgata
     3241 tcaacggcga agccggtgca tgggcacgca tcatgagcgg taccggctct gccagtggtg
     3301 gtttcagtga caactacacg cacgttcagg tcggggtcga caaaaaacac gagctggacg
     3361 gactggattt gtttaccggt ttcactgtca cacacactga cagcagtgcc tccgccgatg
     3421 ttttcagtgg taaaacgaag tctgtggggg ctggcctgta tgcttccgcc atgtttgatt
     3481 ccggtgccta tatcgacctg attggcaagt atgttcacca tgataatgag tacactgcaa
     3541 cctttgccgg actcggaacc cgtgattaca gcacgcattc atggtatgcc ggtgcagaag
     3601 cgggctaccg ctatcatgtc actgaggatg cctggattga gccacaggct gagctggttt
     3661 acggttctgt atccggtaaa cagtttgcat ggaaggacca gggaatgcat ctgtccatga
     3721 aggacaagga ctacaatccg ctgattggcc gaacgggtgt ggatgtgggt aaatccttct
     3781 ctggtaagga ctggaaagtg acagcccgtg ccggtctggg ctaccagttc gacctgctgg
     3841 ctaacggcga aaccgtattg cgggatgcat ctggtgaaaa acgcatcaaa ggtgaaaagg
     3901 acagccgtat gctgatgtcc gttggcctga atgcagaaat cagggataac gtccgctttg
     3961 gactggagtt tgagaaatcc gcctttggta agtacaacgt tgataatgct gtcaacgcta
     4021 atttccgtta ctcgttctga tactccgtat tcaaaagggc ctgcgggccc tttacgctca
     4081 gtgcctgccg ccctgatatg tctgagagac atttccggga tgtcagtcga ctggtttact
     4141 taactgcgat gactaaagct ggcagtaatg ccggcttttt tacggccaca tacgtcttcc
     4201 tctcatccgg aactgtgata caaatcctgc atacaggatt gaccgccggt tcctgcaaat
     4261 ggcacatttt cagtaagata gcgaaggtta catttgtcac tggttaacat accgatggta
     4321 aaaggggcaa gcagcgccag caattcatca gcccgggggc caaaagtgta ggccagaacg
     4381 ccaccagttt tcgtgttgta cgcgtaccag agcctgtgct gtcacgcttt actgccgata
     4441 aaactccatt gctcatcaag ttcgcagatg agtgccatat cagcatgagc gaccggggat
     4501 gacgttattc ttcgtggcga gcgtttttta aagtccgaat gacagtatta atgccgattt
     4561 tcagcgtacg ggcagtatcg cgaactccgg caccgttgaa aggcatttca gtgatttggt
     4621 cttgaactcc cggtttacgg gcctcgtaac ggtaagtgag ctgaaacact cgatgacagt
     4681 cacggcagcg aaatcgatca tgtccttttg ggttcttccc gtgacggtaa acctgaacgg
     4741 actggcaacg ggggcaatga aggttaactg tgaccattaa agaaccttaa aagtgactat
     4801 tataccccta attcaactaa ttggaggcat taccatgcat tagtgcaagc gccttgtgga
     4861 ttcttttgct aatggcggaa acatattgta caatattgca tttatccctc aataaataaa
     4921 tgagttatca gattgcaaaa atctgttatc aaactaattc aaataaaaag agattgatac
     4981 gaccaacgta cacaaaaatt ttcgatattc gaactaaata acagctccta ataccgctac
     5041 actttagcta tccagtctgc gcctcagaaa tggacgatgc cgttgaagga ctggtgtatg
     5101 gcaatgagcc gctttattat cgaattcggt gaccgcttgg acggtcactt cggagaaaag
     5161 gcatttacac agcatcctaa acaggttcag cataggtgat ataccgcctt ttcattttct
     5221 tctccattat ttataaggga ggtgtattat tatgcaatat tattggtttt atccattgtt
     5281 tttagttcat gaatgtttgc aaaaaaataa atgaacaata attttatgta tttaaaataa
     5341 attgatttta cttctgtaat ggtgtcatga ttagcagctt tttaaactaa tatcgacttt
     5401 caataaataa ggacaggtaa tggacaacaa aaatatctga acccgggtga gattcataag
     5461 ttgcacagtg ctgtcttcaa agtgccacac ccggaacgta atcactgcct gctccttatg
     5521 gggtatcttc atggtgtcca agcatcagag ttattaggta taaaactgtc tgatattgat
     5581 ttgcaggccg gtaacctgaa tatccggcgg ctctaaacgg tttgtcaact cagcccctcg
     5641 gctgaaacag gaggtacagt ccattcgtcg ctggctgaaa cttcgggatg aatgggtaga
     5701 agagggatat gaatggttat ttaacctcta tggcaggtct gctacaggta ttactgttgt
     5761 tctggggggc actggtgtgg ttaatatcta cgatgcaaga gattctcacg atggcccggt
     5821 ccatcagtgc atgcggcgcg atattgtgcg agtatttttc agggggagtg gtatccggat
     5881 gcagaaaatt ccgctactcg actggtttta tgcaataaat caggatgata atcttctgat
     5941 tttttactga ggcggatatc tctcgctgtg tttactgaaa ttgcggtatt ctgttttttc
     6001 tgaaaaacag aaaatctgat taaaaagagc atatgcgtta agggaaattt aagttctggt
     6061 tgattaactg ataaaaatat taaaaaatcc ctgttgtcgg taatctccgg accattccgg
     6121 gattacacaa taatatcacc atgtgtgata tctatggatc ctcactgttg tttacgcgag
     6181 gatattttca gaatgcttaa cagacgatta tttttttatg ttgaatgcaa ggcatcttcc
     6241 cgtactgatg tatcatcatg tcagtcgttg tccggggctt gtgacactct cacctgaaac
     6301 attttgtgaa cagatgaagt ggctcgcaga taataactgg cggacggtaa catctgctga
     6361 gcttgaatat ttttatcagg ggggaacgct tccacgaaag agtgtcatgc tcacattcga
     6421 tgatggttat cttgataact ggctcagagc atggccggtc ctgaaggaat ataaccttca
     6481 tgcacatatt tttctcatta cggggctgat tggtaaggga aacgtacgta gcaggcagga
     6541 gcaggaatat tctcaccgcg actgtgaacg gttgattgcc gataaccgtt cagatgaggt
     6601 catgcttcgc tggtctgaag tccgggaaat gcgtgattcc ggactggttg aatttcatgt
     6661 gcacacccat tcacataaac gctgggacag gttgtctgta tcacgtgctg aacagtgccg
     6721 gctcatgaaa gaagatattc tggtgggaaa acaatgtctg acggaaaagc tggggttctg
     6781 tagttcccat ctgtgctggc ctgaaggata ttataatcgg gattatatta atctcgccgg
     6841 taagcttggt ttctcttatt tatatacaac cgaaagaaga atgaactgtc ctgaaaatgg
     6901 ttcattacgc atagggcgta tcagcaccaa agagcgggaa cattccggct ggctgaaacg
     6961 acgccttttt tattatacaa cgccactttt ttcttcagta ctggctcttc ataaggggcc
     7021 aagattacca gataattaat gatgaaaatt ttattcactg aatcttcgtc agatattggg
     7081 ggccaggagc tgcaggcact ggcccagatg actgctcttc aaaagcaggg acattccgtt
     7141 ttacttgcct gcagagaaaa aagtaaaatt gctccggaag ccagaaaaag agggcatgat
     7201 gtcactttta ttcctttcag gaacagcctt caccttcctt ccatcctcag acttcgacgg
     7261 attatcgggg aatttaaacc tgatctggtc atctgccata gtggccatga cagtaatata
     7321 gcaggactga gccgtctgat atgctgtcac cgcttcagta ttgtcagaca aaaaacctac
     7381 atcacccgta agaccagaac gttttccctg aattatctgt gtgatttcat tgttgtgcct
     7441 agctcagcca tgatggcgca tctgatggcg gaaggtgtca ggacaccggt aacggttatt
     7501 ccaccgggtt tcgactggcc cgcattgcat aatgaagcca tgcgaccgtt acccctgcat
     7561 atccacgcct gggctgcatc agcagacaat gttccgctta tcgttcaggt ggggatgcta
     7621 cgtcccgaaa aaggacatga atttatgctg cgtgtattgt atcagctgaa gatggaaggg
     7681 aaatcatttc gctggctcgt ggtgggcgcc gggcgtgaag aatacgaagc acgtctgcgt
     7741 cagcagacag aacacctggg aatgagcgga gatgtgttaa tggcaggtgc ccttttccca
     7801 gcgttaccgg tctaccggat tgccagtgtg gtggtcatgc cttcggaaaa tgaagcgttt
     7861 ggtatggtac tggctgaagc gtcagtctcc ggcgtgcccg ttatcgccag tgagacgggc
     7921 gggatccctg atgtcattca gaagaatgtg acagggacgc tgttacccgt gggggatgtg
     7981 tctgcatgga ccggcgcact gcgcgatttt ttatcccgac cggaacgctt ccggatgatg
     8041 gcagccagcg ccagagaaga tattgagtac cggtttgata ttaacagaac ggcacagatc
     8101 attgtgtctc tggctagtca ggctaagggg aaatgcaaca gataacggcg gatcctcttt
     8161 tcagcccgaa ttttgttcag cggttttccg gtaacggagt aatttatcac attgtttaaa
     8221 tacggggacg gtcaatgcat ttaaataccg gacagaacag gcctactttc agctggagcg
     8281 cgctgggatg ggcaattttt tattttggct ttttttccac tctcctgcag gtcatcatct
     8341 tcagcagtgg gtacagcgga acgaatggaa tacgggactc actgttattc agttgtctgt
     8401 ggttgatccc ggtgtttctc tatcctgatc ggataaaaat aattgcagct gttgtcggtt
     8461 tcattctctg gggcacgtcg ctggcagcac tttgttatta ttttctctat ggtcatgaat
     8521 tctctcaaag tgttcttttc gttatgtttg aaacgaatgc cagagaggct ggtgaatatt
     8581 tcagccagta tttcagcctt aaactgttgc ttatctcgct ggtatatact gcggtgtccg
     8641 tttttctgtg gacacgtctg cgtcctgtat atattccttt gccatggcgg agaattgtct
     8701 ctttcctgct gctttatgct ctgcttctgc atccggttgt tctgaaatcg ttaatcagac
     8761 aggagccgct gaatgatact ctaggcaaac tggcatcccg aatggagcct gctgccccct
     8821 ggcagtttgt atccagttat tatcagtacc atcagcaact gaatgcactg acaaccttcc
     8881 tgaatgaaaa tagcgcactg ccaccactgg gtaatctcag ggatgaatca ggggagagac
     8941 cacgcacact ggtcctggtg attggtgagt cgacacagcg cgaacgcatg agcttatacg
     9001 ggtatctacg tgaaacgacg ccggagctgg atgcactgcg taaaaccgat ccgggtctta
     9061 ctgtgtttaa taatgtggtg gcatcgcgtc cgtacaccat tgaagcattg caacaggccc
     9121 ttactttcgc caacgaaaag aatcctgatc tgtatctgac gcagccgtcg ctgatgaaca
     9181 tgatgaagca ggcaggctat aaaacattct ggattaccaa ccagcagaca atcacagccc
     9241 gtaacaccat gctcactgta ttttcgcgcc agacggacag gcagtactac atgaatcagc
     9301 agcgaacaca aagtgcgcgt gaatatgaca ctaacgtgct gaagccgttc cgggaagtgc
     9361 tgaatgaccc tgcaccaaag aaactgatca tcgtgcatct gctgggtacg cacattaagt
     9421 ataaataccg ttatccggaa ggtcaggggc ggtttgatgg cattacaggg catattccca
     9481 ctggattaaa tgcgaaagag ctggaagtgt ataacgatta cgataatgcc aatctgttta
     9541 acgatcatgt ggtggccagt ctgataaagg acttcagagc aactgcgccg gacggctttc
     9601 tgctttattt ttcagaccat ggtgaagaag tatatgatac tccgccatat aaaacgcagg
     9661 gacggaatga agataacccc acacgtccca tgtacactgt tccgttcctg ctgtggacct
     9721 cggaaaagtg gcatgctgca catccgcgag atttttcgca gtatgttgac cgcaaataca
     9781 gtctggctga actgatccac acctggtcag atttggcggg actgacatac gatggttacg
     9841 atccgacccg ttctctggtg aatccgcaat tcagggaaac cacccgctgg attggaaatc
     9901 cgtataaaaa gaatgggctc actgatttcg acactcttcc gtatggtgag ccatagtatt
     9961 ttcttatctt cggttccgtt ctgaaggcag gaggatgtgt tttcctctgc tgtccggtgg
    10021 cctccaggga tggtaccacc ttctgctgta ctctgttatc ataatgagat aagcaggcag
    10081 ataccggtaa atatgaaaaa tatcagaact gagtttattc cccgctttaa ccttacatta
    10141 tgcttccccc gttactggat gacgtggaca ggtatcggta ttatctgtgt gtttgcaatg
    10201 gtcccccccg cactgagaga tccattgttg ggaaaattgg gcatgctggt tgggcgtctg
    10261 gggaaaagcg cacgtcaacg ggccttgatt aatttatcgc tttgttttcc ggagtacagt
    10321 gataaagaaa aggagaatat cgttgatgcc atgtttgcaa cagcctccat ggccgttgtg
    10381 cttatggctg aattagcact gtccgggcct gataaaattt cgcatcgtat acgctggaat
    10441 ggtctggaga ttgtggagaa gatggcacag aacaatgaaa aggtcatttt tcttgtgcca
    10501 catgcctggg gagtggatat tcccgcaatg ctgatggctg cgtcgggcag aaaaatggca
    10561 gcgatgtttc ataaccagag aaatccggtg gtggattatg tctggaacag tgttcgtcgt
    10621 cgtttcggag gaaaactgca cgcacgtaat gacggtattg cctcctttgt ccgctcagtc
    10681 cggcagggat actggggata ttatcttcct gatcaggatc atggcccgga attcagtgag
    10741 tttgcagatt tttttgcgac gtataaagca actttaccgg ttatcggacg gctcagcagg
    10801 atcagtggtg cacggataat accgcttttc cctgtttatg acggaaagac ccatcatctg
    10861 actattcatg tcagcccgcc acttgctata cgacagaaga gtgatgctca tattgcgcga
    10921 cagataaacg aagtggttga aaatttcgtt cgtccacatc ctgagcagta tacgtggatc
    10981 ctcaagttgc tgaaaacgcg taaagaaggg gaagaggatc cttactgagg cagctttttc
    11041 aggataatga gcttacccag caatagtgga cacacgctaa gtgagtaaac tctcagtcag
    11101 aggtggctca tatgacaaaa aaacagtacc aaccagtaaa aaaccccgta aatagcattc
    11161 gcctgaattt cgcagtgaag ccctgaagct tgctgaacgc atcggtgttg ctgtcgcagc
    11221 tcgtgtgctc agtctgtatg aatcacagct tgtaagcggc tcgtcagaac tgtattgata
    11281 tttactgagc ggtcagttca acgtaagcga gtgttatgcg ccatggaagc ttacccagga
    11341 atatgttatc aggcctcagc atggcgaagt gttgcaacgc tggaagcaga atga
//
LOCUS       DAZFAB010000130        11382 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZFAB010000130 DAZFAB010000000
VERSION     DAZFAB010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11382
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.130"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            77..211
                     /locus_tag="ACHJWA_004107"
     CDS             77..211
                     /locus_tag="ACHJWA_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFO4310596.1"
                     /translation="MIYYLGYDRFLLIFFTDYSHFMDRITLPSTDTARGKGKWKIITA
                     "
     gene            190..294
                     /gene="speFL"
                     /locus_tag="ACHJWA_004108"
     CDS             190..294
                     /gene="speFL"
                     /locus_tag="ACHJWA_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFO4310597.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            605..2803
                     /gene="speF"
                     /locus_tag="ACHJWA_004109"
     CDS             605..2803
                     /gene="speF"
                     /locus_tag="ACHJWA_004109"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFO4310598.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVSDVE
                     RGKLDEIDATGYDIPVFIATENEERIPAEYLSRISGVFEHGEARKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEALFRADLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELITEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFIPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2800..4119
                     /gene="potE"
                     /locus_tag="ACHJWA_004110"
     CDS             2800..4119
                     /gene="potE"
                     /locus_tag="ACHJWA_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFO4310599.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSHVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4176..5816)
                     /gene="pgm"
                     /locus_tag="ACHJWA_004111"
     CDS             complement(4176..5816)
                     /gene="pgm"
                     /locus_tag="ACHJWA_004111"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFO4310600.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKAVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5842..6387)
                     /gene="seqA"
                     /locus_tag="ACHJWA_004112"
     CDS             complement(5842..6387)
                     /gene="seqA"
                     /locus_tag="ACHJWA_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFO4310601.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPIKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6572..7336
                     /gene="ybfF"
                     /locus_tag="ACHJWA_004113"
     CDS             6572..7336
                     /gene="ybfF"
                     /locus_tag="ACHJWA_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO4310602.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIAHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFSAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7476..7769
                     /gene="ybfE"
                     /locus_tag="ACHJWA_004114"
     CDS             7476..7769
                     /gene="ybfE"
                     /locus_tag="ACHJWA_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFO4310603.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7909..8439
                     /gene="fldA"
                     /locus_tag="ACHJWA_004115"
     CDS             7909..8439
                     /gene="fldA"
                     /locus_tag="ACHJWA_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFO4310604.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8649..8735
                     /gene="uof"
                     /locus_tag="ACHJWA_004116"
     CDS             8649..8735
                     /gene="uof"
                     /locus_tag="ACHJWA_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFO4310605.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8728..9174
                     /gene="fur"
                     /locus_tag="ACHJWA_004117"
     CDS             8728..9174
                     /gene="fur"
                     /locus_tag="ACHJWA_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFO4310606.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9258..9584)
                     /gene="chiQ"
                     /locus_tag="ACHJWA_004118"
     CDS             complement(9258..9584)
                     /gene="chiQ"
                     /locus_tag="ACHJWA_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFO4310607.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9634..11039)
                     /gene="chiP"
                     /locus_tag="ACHJWA_004119"
                     /pseudo
     CDS             complement(9634..11039)
                     /gene="chiP"
                     /locus_tag="ACHJWA_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
BASE COUNT         2790 a         2792 c         2820 g         2980 t
ORIGIN      
        1 ttaacgcaaa tataaaacac gaaattacaa catttaagta acaaaacagc atctagtatg
       61 gcgctattca taattaatga tttattatct ggggtatgat cgttttttgt tgatcttctt
      121 cacagattat agccatttca tggatagaat aactctacct tcaactgaca cagcaagagg
      181 taaaggtaaa tggaaaataa taaccgctta atgcctcata taaggcggac aacccatatc
      241 atgaagtttg cccatcgtaa tagttttgac tttcatttct ttaatgcccg ctagtcttct
      301 gactaaaggg caccccagcg tacaggtctc cctgacttta agcattacag gttaatacct
      361 gtatccctcg gtgctcatat actgctaacc cttttaaact ctacataatt cgagtcgcag
      421 catatgcaac ttgaagtatg acgagtatag ccagttaccg ggctggtctg ggttattgca
      481 tctgcaaaaa gcaaactact gatttattta tcagcggtgg agctttgctt tttttccggc
      541 gtgatcgatt tctcctttga gaaattgagg acctgctatt acctgaaata aagagatgaa
      601 caaaatgtca gaattaaaaa ttgccgttag tcgttcttgc ccggattgtt tttccactca
      661 tcgtgcatgc gtgaatatag acgaaagtaa ttatattgac gttgccgcca ttattttatc
      721 agtcagtgat gttgaacgtg gaaaactcga tgaaatagac gctactggct atgacattcc
      781 tgtttttatt gcaacggaaa atgaagaacg tataccagca gaatatcttt cacgtatttc
      841 tggtgtcttc gaacatggag aagcacgcaa agaattttat ggtcgtcagt tagaaaccgc
      901 tgccagccat tatgaaactc aactgcgccc acctttcttt cgcgcactgg tcgattatgt
      961 caatcagggc aacagcgcat ttgactgccc cggtcatcag ggcggcgaat ttttccgtcg
     1021 ccatccggcg gggaatcagt ttgtggaata ctttggtgag gcgctgttcc gtgccgactt
     1081 gtgcaacgcc gacgtagcga tgggcgatct gctgattcac gaaggcgcgc catgcattgc
     1141 acagcaacat gcagcaaaag tgtttaatgc cgataaaacc tacttcgttt taaatggcac
     1201 ttcatcttct aacaaagtgg ttttaaacgc cctgctgaca ccgggtgatc tggtgctatt
     1261 tgaccgcaat aaccataagt ctaaccacca cggggccttg ctacaggctg gtgcaacacc
     1321 ggtttatctg gaaacagcac gtaacccgta tggttttatc ggtggtattg atgcacactg
     1381 cttcgaagaa agttatctgc gtgaactgat tacagaagtt gcaccgcaac gggcaaaaga
     1441 ggcgcgtccg ttccgcctcg ctgtgattca gctaggcacc tacgacggta cgatttataa
     1501 cgcccgccag gtggtggata aaattggtca tctgtgtgac tacatcctgt ttgattccgc
     1561 atgggtcggc tatgaacagt ttattccgat gatggctgac tgttcgccgc tgttgctgga
     1621 tcttaatgag aacgatccgg gtattctggt tacgcaatcc gtacataaac agcaggctgg
     1681 tttttctcaa acttcacaaa tccataaaaa agacagccac atcaaagggc aacagcgtta
     1741 tgtaccgcac aaacgcatga acaacgcctt tatgatgcat gcctccacca gcccgttcta
     1801 tccgctgttt gccgcactgg atatcaacgc caaaatgcat gaaggtgtca gcggtcgtaa
     1861 tatgtggatg gattgtgtgg taaatggcat taatgcccgc aaactgatcc tcgataactg
     1921 tcagcatatt cgtccgttca tacctgaact ggtggatggt aaaccctggc agtcgtatga
     1981 aacagcgcaa attgcggttg atctgcgctt cttccagttt gtaccaggtg agcactggca
     2041 ttcttttgaa ggctatgcag agaatcaata ctttgttgat ccgtgcaaac tgttgctgac
     2101 aaccccaggt attgatgcac gtaacggcga atatgaagcg ttcggtgtac ccgcgacgat
     2161 tcttgctaac ttcctgcgcg aaaatggcgt agtgccggaa aaatgcgatc ttaactccat
     2221 cctcttcctg ctgactccgg cagaagatat ggccaaactt cagcaacttg ttgccctgct
     2281 ggtacgcttc gaaaaactgc tggaatctga tgcgccgctg gcggaagtgc taccttccat
     2341 ctacaaacag catgaagagc gctacgccgg ttataccctt cgtcagttgt gccaggaaat
     2401 gcatgatttg tatgcccgcc acaacgtgaa acaactgcaa aaagagatgt tccgtaagga
     2461 acacttccca cgcgtcagca tgaatccgca agaagctaac tacgcctatt tacgcggtga
     2521 agtggagctg gttcgtctgc cggatgcaga aggccgtatc gctgccgaag gtgcgcttcc
     2581 ttatcctccg ggtgtgctgt gtgttgttcc gggtgaaatc tggggtggtg ctgttttgcg
     2641 ttacttcagc gctctggaag aagggatcaa cctgctgcca ggttttgcac cggagctgca
     2701 gggtgtctat atcgaagaac atgatggtcg taagcaagtt tggtgctatg tcatcaagcc
     2761 tcgtgatgcg caaagcaccc tgctgaaagg ggaaaaatta tgagtcaggc taaatcgaac
     2821 aaaatgggcg tcgttcagtt aaccatactg acgatggtca acatgatggg ctccggtatc
     2881 atcatgctgc cgacaaagct tgccgaagtt gggacaatct caattatctc ctggctggtg
     2941 acagccgtag gctcaatggc actggcatgg gcattcgcaa aatgcggtat gttcagccgt
     3001 aaatcaggcg gtatgggcgg ttatgccgag tatgctttcg gtaaatccgg taactttatg
     3061 gcgaactata cctacggcgt ctcactgctg attgctaacg tcgcgattgc tatttcggcg
     3121 gttggttacg gcaccgaact gctcggcgcg agtttgtcgc cagtgcagat tggtcttgcg
     3181 accatcgggg tgctgtggat ttgtaccgtg gctaactttg gtggtgcgcg cattaccggg
     3241 caaatcagta gcattactgt gtggggagtc attattccgg tcgtcggtct gtgcattatt
     3301 ggctggttct ggtttagccc gacgctgtac gttgattcct ggaatccgca tcatgcaccg
     3361 ttcttcagtg cggtaggttc ttccatcgct atgacgctgt gggcttttct tggtctggag
     3421 tctgcgtgtg caaatactga tgtagtggaa aacccggaac gtaatgtgcc aatcgcggta
     3481 ctcggcggta cgttaggtgc ggcggtgatt tatatcgtct ccaccaacgt gattgccggg
     3541 attgtgccaa atatggagct ggcaaattca acggcaccat ttggtctggc cttcgcgcag
     3601 atgttcacgc cggaagtggg taaagtcatt atggcgctga tggtgatgtc ctgctgcggt
     3661 tcgctgcttg gctggcagtt caccattgcc caggtgttta aatcttcatc tgatgaaggc
     3721 tacttcccta aaattttctc ccatgtaacc aaagtggatg caccggtgca gggaatgttg
     3781 accattgtga ttatccagag cgggttggcg ctgatgacga ttagcccgtc gctaaacagt
     3841 cagttcaacg tgctggttaa cctggccgtg gtgaccaata tcattccgta tattctgtca
     3901 atggcggcat tagtcattat tcagaaggtc gctaatgtgc ccccctcaaa agcgaaagtt
     3961 gcaaactttg ttgcttttgt tggcgcgatg tacagctttt atgcgctgta ctcatccggg
     4021 gaagaagcca tgctgtacgg ttccatcgtg accttccttg gttggacact gtatggtctg
     4081 gtatcaccac gctttgaact gaaaaataaa cacggttaaa aaagcaaagt aaaaaagggc
     4141 gatcttgcga ccgccctttt tcttatttaa tgtgtttacg cgtttttcag aacttcgcta
     4201 acaatctcaa ccgcttcttt ctcaatctgc ttgcgatgtt cttcaccgag gaagctttcg
     4261 cagtagatct tatatgcgtc ttccgtgcct gacggacgcg cggcgaacca gccgttgtca
     4321 gtcatcactt tcagaccgcc aatagaagca ccgttgcccg gagcagcagt caggcgcgcg
     4381 gtgatcgggt caccagccag ggtgctggca ctcaccattt ccggagacag cttagacagc
     4441 gccgcttttt gtgcggaagt cgcagctgcc tgcaaacggt tgtagctcgg cgcaccaaag
     4501 cgttttgcca gttcgttgta gtgttcctgc gggttcttac cggtgacagc ggtgatttcc
     4561 gccgccagca gacacatgat gatgccgtct ttgtcggtgg accacggcgt gccgtcgaaa
     4621 cgcaggaagg aagcccctgc gctctcttcg ccgccaaagc cgaagctgcc gtcgaacaga
     4681 ccatcaacaa accatttgaa acctaccggg acttctacca gtttacggcc caagtcgttg
     4741 accacacggt cgatcatcgc agatgaaacc agcgttttac cgacggcaac agctttgccc
     4801 cactgcggac gatgctggaa caggtagttg attgccaccg ccaggtagtg gttcggattc
     4861 atcaaacctg ccggagtgac gataccgtga cggtcataat ccgggtcgtt agcaaacgcc
     4921 agatcgaact tatcacgcag tgccagcagg cccgccatcg cacactcgga ggagcagtcc
     4981 atacggatcg cgccgtcttt atcaaggtgc ataaagcgga aggtttgatc gacctgatcg
     5041 ttaacgatgg tcaggttgag gttgtaatac tcgccaatac gcttccagta ttcgataccg
     5101 gaaccgccca gcggatcaac gccaagcgtc aggcccgctt tctgaatcgc cgccatatca
     5161 acgatatcgg ccagcccttc cacaaacggc tgcaccagat cctgctcttt cacatgaccg
     5221 gatgccatcg cttcgtcgag ggagatacgc ttcacgcctt tcaggccatc ggccatcagt
     5281 gcgttggctc tgtcttccac cactttagtg acgttggtat cagccgggcc accatttggc
     5341 ggattgtact tgataccacc atcttccggc gggttatggg acggtgtaat aacgataccg
     5401 tctgccagcg ggccaccttt tttattgtga accaggatgg cattggaaac ggcaggcgtc
     5461 ggggtgaagc cattgttttc ctgcacaata acatctacgc cgttcgctgc cagcacttcc
     5521 agaacggaaa taaatgcagg ttcggacagg gcgtgagtat ctttacccac atagcaaggg
     5581 ccagtgatgc cgtttttcgc acgttcttca gcaattgcct gagcgatcgc cagaatgtgc
     5641 ggctcgttaa agctgtggcg cgctgcactg ccacggtgac cggaagtacc gaatttcacc
     5701 gcgtgctccg cattccctgc ttctggtttc agtacataat attgcgccgt cagttgggcg
     5761 acgttaatca aatcactctg ttgtgcaggt tggcctgcac gattgtggat tgccattgct
     5821 ttgtcctttg tctgcaacgt tttagatagt tccgcaaacc ttctcaatca attccgccgg
     5881 gaattgcatc gactgcatga tgtgttcgat catgctgcat ttacggccgg tgttggtgtt
     5941 ggtgatcacc caatacggcg tgcctggcac atgtttcggc ttggtctgat taccattttt
     6001 cagcagcgtt tgttcatctg ccgcaaagta aacgcgtgta cgaccgtgca acgattccgt
     6061 tgcttcggca aacgcctggg cgtcaagaga atatagtgta gacaacagca gcataaagcg
     6121 attgaccgct cgcttttgct ctgcatattc atccgaaagc agaagttcac gcattgcgcg
     6181 aaccttgtct ttaatcgttt tgatcggctt cgcttcgacg atagcaggtg acgcaacgcg
     6241 aacctctttc gtcaccggag cagcaggctg tgatgcggcg gaaaatttca acatacgccg
     6301 taaaatgtcg gatgcgctct cgccgatatg cttagtgtgg ctggcaatat agctgtagag
     6361 ttcatcatca acttcaatcg ttttcatctt aatccagtgc agtgtcttag ctgaatacaa
     6421 gtcgccagga gtatatgaat aaatcccggc agcggatagc gtcaagcctg gcttgcagaa
     6481 aaactcggaa taaaccttat ttgttgcagc cctgtggcac aatggtcaac ataataccct
     6541 aacccgacaa cgcgaaaaaa agaactttgc catgaaattg aatatccgcg cgcaaactgc
     6601 acaaaaccag cacaataatt ctcccatcgt tcttgtccat ggtctgtttg gcagcctcga
     6661 caaccttggc gtactggctc gcgatctggt aaacgatcac aatatcatcc aggttgatat
     6721 gcgtaaccac ggtctttcac cgagagatcc ggtaatgaat tacccggcga tggcgcagga
     6781 tcttgttgat actctggatg cacagcagat cgacaaagca acatttatcg ctcactccat
     6841 gggcggtaaa gcggtaatgg cacttactgc actggccccc gatcgcatcg ataaactggt
     6901 ggcgatcgat atcgcaccgg tcgactatca cgtacgccgt catgatgaga ttttctcggc
     6961 tatcaacgcg gtcagtgaat cggacgcaca aactcgccag caagcagcag caataatgcg
     7021 ccagcatctt aatgaagaag gggtgattca gtttctgttg aaatcttttg ttgacgggga
     7081 gtggcgcttt aacgtgccgg tattgtggga tcagtatccg catattgtgg gttgggagaa
     7141 aatcccggca tgggatcacc ctgccctgtt tatccctggt ggtaattctc cgtatgttag
     7201 cgagcagtac cgtgatgatt tactggcaca atttccacag gcacgggcgc atgtgattgc
     7261 aggcgcgggt cactgggtcc atgctgaaaa accggatgcg gtattacgcg ctatccgtcg
     7321 ctatctcaat gattaacgca actgattaaa aacccagcgt ccgcgtcgct ttccgcgcgt
     7381 gggcgttggc gtgactcatc gcgctgatgt attatggcgc gctatccatc cgggcagagg
     7441 cctggctgat tgtttccccc gaagtcacca agatcatggc caaagaacaa acggaccgta
     7501 cgacattaga tctgttcgcg cacgagcgtc gaccgggacg accgaaaaca aatccgcttt
     7561 cgcgcgatga acagctgcgt attaataaac gcaaccaact aaaacgcgac aaagtacgtg
     7621 gcctaaagcg tgtcgaactg aagctgaacg cggaagctgt cgaggcgctg aacgagctgg
     7681 cggaatcgcg caatatgagc cgtagcgaac tgatcgaaga gatgttaatg cagcaactgg
     7741 cggcgctgcg tagtcagggc atcgtttaaa tttccacttt catgtagcac agtgtgcagt
     7801 cctgctcgtt tgctgattcc gcaagactgc ctgttctgct atgattgcct ttatccgtgg
     7861 gcaattttcc acccccattt caataagttt caagaggtta tttcactcat ggctatcact
     7921 ggcatctttt tcggcagcga caccggtaat accgaaaata tcgcaaaaat gattcaaaaa
     7981 cagcttggta aagacgttgc cgatgtccat gacattgcaa aaagcagcaa agaagatctg
     8041 gaagcttatg acattctgct gctgggcatc ccaacctggt attacggcga agcgcagtgt
     8101 gactgggatg acttcttccc gactctcgaa gagattgatt tcaatggcaa actggttgcg
     8161 ctgtttggtt gtggtgacca ggaagattac gccgaatatt tctgcgacgc attgggcacc
     8221 atccgcgaca tcattgaacc gcgcggtgca accatcgttg gtcactggcc aactgcgggc
     8281 tatcatttcg aagcatcaaa aggtctggca gatgacgacc actttgtcgg tctggctatc
     8341 gacgaagacc gtcagccgga actgaccgct gaacgtgtag aaaaatgggt taaacagatt
     8401 tctgaagagt tgcatctcga cgaaattctc aatgcctgat gtgatgcggc gtagactcat
     8461 gtctacgccg tattaataga taatgccaat caaaataatt gctacaaatt tgtaactttt
     8521 gccgttgtac ctgtacaatg tcccggtgtt caagtggcct tgccgttgta aatgtaagct
     8581 gtgccacgtt tttattaaca atatttgcca gggacttgtg gttttcattt aggcgtggca
     8641 attctataat gatacgcatt atctcaagag caaattctgt cacttcttct aatgaagtga
     8701 accgcttagt aacaggacag attccgcatg actgataaca ataccgccct aaagaaagct
     8761 ggcctgaaag taacgcttcc tcgtttaaaa atcctggaag ttcttcagga gccggacaac
     8821 catcacgtca gtgcggaaga tttatacaaa cgtctgatcg atatgggtga agaaattggt
     8881 ctggctacgg tatatcgcgt actgaaccag tttgacgacg ctggtatcgt cacccgccac
     8941 aattttgaag gcggtaaatc cgtatttgaa ctgacacagc aacatcacca cgatcacctg
     9001 atctgcctcg actgcggcaa ggttatcgaa tttagtgatg attccatcga agcgcgtcag
     9061 cgtgaaattg ccgcaaaaca tggcattcgc ctgactaacc acagtctcta tctttacggt
     9121 cactgcgccg aaggcgattg ccgcgaagat gagcacgcgc acgaaggcaa ataagccagt
     9181 ctgaacgaga aaagccaacc tgcgggttgg cttttttatg caagggaaat aagaaaagcg
     9241 agagttacag ctctcactta tttgttatta ctgcgaattt cctgccagac cttatcacaa
     9301 tcggctttca ccgcctgatc atttccggtt tgcgttgcac gcagacactg ctgataatcc
     9361 agtacgcgga cactttcctg gtcggcaaac tgctgatgtt gtttctcttt cttcagcacg
     9421 tttaacacgc tctggcaggc ttcaattttt tccggcgaac cttgtgcggt gttgatacag
     9481 gcgctgtatg cctctttcag acggctgtct tcctgtggcg cggttgattg cgcacaagct
     9541 accagccccg atgccatcat ggcgatgaga atcagttttt tcatagcaaa ttcctcaaac
     9601 gtgcggcagg cacaaaacct gccgcgtcgg gcatcagaag atggtgaatg gtgcgattac
     9661 cataaatttt acgtcacgct catcctggaa gatgttgccg taaccgccgc cccagcttgg
     9721 gatgtcggag tggttgtcgt attcggtgaa gtgcagtttg aacatcgtgc ctttggcgcg
     9781 accgtcctga atggtgtaga ccgcatccag gctgtatgca gactcttcaa tagtccggtt
     9841 tttgtcgtag tacgcatccg gattgctctg ccaggtcgca ggtttagcat cccatgcgta
     9901 aacgtaggaa gcgccgatgg cgaagcctgg aagattccag tttttcaggt catacatcgc
     9961 accgaagaag accgcttttt cgccgttggc gttgaagtca gaacggttat cccaccagat
    10021 atccaggcga ccgttcgagg aagcgtaggt tggagtcata cgttgcagga agtatccctg
    10081 ctgaccgtca gctttaaccc aggtgccttc gaggcgcaaa tccactacgt cagccgcccg
    10141 gtaaccaaag gtcaacgctt gcagccaggc ggtgccgtca taaagatcgt tgacgctgcg
    10201 atcgtcaact ttatcgcgcg taccgtagaa ctggtagctg gtggttaacg ggctaccggc
    10261 gatatcaaat ttgtagctgg ctttggcaaa gtattgatcg atataccctt ccgcctgacc
    10321 aaatgccgct tccagtacga agttattttt gaagtcgtat ttcgccccaa gggagtgcag
    10381 ataatcaact ttggtggttt tatcgttctg ataaaactca tccatttcca gatgccacgg
    10441 tgctttgtat tcgttggtcc acatgtagga gaaactcaac gcaccagcat cgccgtaatc
    10501 aaaattcgcc ccggcctccg caccctgata agtacccggc ataaagctcc agtgcggcgc
    10561 taacagcgtt tgaccagttg gctgaatata acctgccctc gccacaccgg accgtattta
    10621 aatttggccg ctgctttata caggcttata ccgcttttat cgccggacca gtcttcgtca
    10681 taggctttat tactttttga aaacgcgatt tcgttcgggt gagagctgtc gccgttttcc
    10741 gccatttcaa tcgccgtaaa cgcggcaata tcaaggccga acatatcagc agcataaccg
    10801 gactgaaaat cgaggttggc gttccaggtg gagtgagaaa ggttggtttt gtatttatcg
    10861 ccgtcggtaa catctttacg gtcgcgttca cgctgccagt aatagatacc gccggttaag
    10921 gttgaatcgt caatgaatcc ttcggcgcga gcctccggca ttgccataaa gcccgacatt
    10981 gctgtaacac cggcgatagc cagcgccagc gtactacgtt tgccactaaa cgtacgcatg
    11041 ggttaatcct ctttgacgta taaattgctg caccacaggt gaacagcgaa aaaaataaaa
    11101 attaaaaaag tagcgttgag aaaagtctcg ctgactacag gaacagacta tttttcgcga
    11161 cgcgaattat caatctcttt ctgtaaccag aatgtaagaa catgagcaac cgcacatatt
    11221 ttgatgattt ttgttacaag gcttaagttt gcggttgaaa ggcttgcaga agaataaaag
    11281 cgtgaaattc cagcaagatg aaagcggtta cattttatct aattatgttt tgtgttttta
    11341 aatttatttt atggataaat tgcgaagcgg aattaattcg cg
//
LOCUS       DAZFAB010000131        11141 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZFAB010000131 DAZFAB010000000
VERSION     DAZFAB010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11141
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.131"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            363..977
                     /gene="evgA"
                     /locus_tag="ACHJWA_004120"
     CDS             363..977
                     /gene="evgA"
                     /locus_tag="ACHJWA_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFO4310608.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            982..4575
                     /gene="evgS"
                     /locus_tag="ACHJWA_004121"
     CDS             982..4575
                     /gene="evgS"
                     /locus_tag="ACHJWA_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFO4310609.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPLRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     ESPFKDVFSNTHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASEHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEIIQQVAAVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNVDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLNCGMNLCLFKPLTLDVLKTHLSQLHQVAHIVPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQQNN"
     gene            complement(4631..5776)
                     /gene="yfdE"
                     /locus_tag="ACHJWA_004122"
     CDS             complement(4631..5776)
                     /gene="yfdE"
                     /locus_tag="ACHJWA_004122"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFO4310610.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGLSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQTILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(5850..6794)
                     /gene="yfdV"
                     /locus_tag="ACHJWA_004123"
     CDS             complement(5850..6794)
                     /gene="yfdV"
                     /locus_tag="ACHJWA_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFO4310611.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(6864..8558)
                     /gene="oxc"
                     /locus_tag="ACHJWA_004124"
     CDS             complement(6864..8558)
                     /gene="oxc"
                     /locus_tag="ACHJWA_004124"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFO4310612.1"
                     /translation="MSDQLQMTYGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(8612..9862)
                     /gene="frc"
                     /locus_tag="ACHJWA_004125"
     CDS             complement(8612..9862)
                     /gene="frc"
                     /locus_tag="ACHJWA_004125"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFO4310613.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPDWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(10375..11010)
                     /locus_tag="ACHJWA_004126"
     CDS             complement(10375..11010)
                     /locus_tag="ACHJWA_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFO4310614.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AAERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKK
                     TNVNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVM
                     ENQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT         3264 a         2505 c         2293 g         3079 t
ORIGIN      
        1 tcattcttca tatcaaacac agtgcatgtg ttgcacatta tccctggaac gttggaggcc
       61 taatttaaat gaggtgcaaa aaaaacaaca gaggactaaa ccgtggcttt tgcaatacaa
      121 ttcttacgcc tgtaggatta gtgagaagac ttatagtgct aacttgaaac tataaatcat
      181 cggtataatc cctgatttta ttgttgacat ttcatatatg ccaactattt atatggtata
      241 cttgtcgaat tatcttaaag gaagctcaaa ttttcttatt tttattgaga aaatgagatg
      301 atgccttatg tctgtattac tacagggaga agggagatgc ttcattgcaa agggaataat
      361 ctatgaacgc aataattatt gatgaccatc ctcttgctat cgcagcaatt cgtaatttat
      421 tgatcaaaaa cgatattgaa atcttagcag agttgactga aggcggaagt gccgttcagc
      481 gggtggaaac acttaagcct gatatcgtca tcattgatgt cgatatcccc ggagttaacg
      541 gtatccaggt gttagaaacg ctgaggaagc gccaatatag cggaattatt attatcgtct
      601 ccgctaaaaa tgaccatttt tacgggaaac attgtgctga tgctggcgct aatggtttcg
      661 tgagtaaaaa agaaggcatg aacaatatca ttgcggctat tgaagctgca aaaaatggct
      721 actgctattt ccccttctct ctcaaccggt ttgttggaag tttaacgtcc gaccagcaaa
      781 aactcgactc cttatcgaaa caagaaatta gtgtcatgcg gtatattctt gatggcaagg
      841 ataataatga cattgctgaa aaaatgttca tcagcaacaa aactgtcagc acttataaaa
      901 gtcgcctgat ggaaaaatta gaatgtaaat cactgatgga tctttacaca ttcgcacaac
      961 gtaacaaaat cggctaacca catgaagttt ttaccctata tttttcttct ctgttgtggt
     1021 ctttggtcga ccataagttt cgcagacgaa gattacatcg aatatcgtgg catcagtagt
     1081 aacaaccgtg tcacacttga tccactacgt ctgagcaaca aggaattacg ttggttagcg
     1141 agcaaaaaaa atcttgtgat tgcagtacac aagtcccaaa cggctacgtt gttgcatacc
     1201 gattcgcagc aacgggttcg tggtattaat gctgattatt taaatctttt aaaaagagcg
     1261 ttaaatatca aattaacact ccgggaatac gcagatcatc aaaaagcaat ggacgcgctg
     1321 gaagaaggtg aagtcgatat agtgttatca catttagttg cttcgccgcc tcttaatgat
     1381 gacattgctg caaccaaacc actgataatt acctttccgg cgctggtcac cacccttcac
     1441 gattcaatgc gaccgcttac ctcatcaaaa ccagtaaata ttgctcgagt agcaaattat
     1501 ccccctgacg aggtaattca tcaatcattt ccaaaagcaa caattatctc ttttacaaat
     1561 ttatatcagg cattagcatc cgtctcagcc ggacagaatg attactttat tggtagtaac
     1621 atcattacca gcagtatgat ttcccgctat ttcactcact ccttaaatgt agtgaaatat
     1681 tataactcac cgcgtcaata taacttcttc ttaaccagaa aagaatctgt cattcttaat
     1741 gaagtactca atcgatttgt tgatgcttta acaaatgaag ttcgctatga agtatcacaa
     1801 aattggcttg atacaggaaa cctggccttt ctgaacaagc cattagaact cactgaacat
     1861 gaaaaacagt ggattaagca gcatcccgat ttaaaggtgc tggaaaatcc ttactcgccc
     1921 ccctattcta tgacggatga aaatggctcg gttcggggcg ttatggggga cattcttaat
     1981 attattacct tgcaaacagg tttaaatttt tctccgatca ccgtttcaca caatatccat
     2041 gctggaacac agctgaatcc cggcggatgg gatatattac ccggtgctat ttatagtgaa
     2101 gatagagaaa ataatgtttt atttgctgaa gccttcataa caacgcctta cgtttttgtc
     2161 atgcaaaaag cgcctgacag tgaacaaaca ttaaaaaaag gaatgaaagt tgccattcca
     2221 tattattatg agcttcattc gcaattaaaa gagatgtatc cggaggtaga gtggataaaa
     2281 gtcgataacg ccagcgctgc atttcacaag gtcaaggaag gcgaacttga tgctctggtc
     2341 gcgacacagt taaattcacg ttacatgatc gaccattact atcctaatga actgtatcat
     2401 tttcttattc ccggcgttcc gaatgcatcg ctttcgttcg cttttcctcg cggagaaccg
     2461 gaacttaagg atattattaa taaagcactg aatgcaatcc ccccaagcga agttctgcgc
     2521 ctgaccgaaa aatggattaa aatgcccaat gtgaccattg acacatggga cctctatagc
     2581 gagcaatttt atattgttac gacattatcc gttttattag ttggcagtag ccttttatgg
     2641 ggattctacc tgttacgctc agttcgtcgt cgtaaagtca ttcagggtga tttagaaaac
     2701 caaatatcat tccggaaagc gctctcggac tccttaccga atccaactta tgttgtaaac
     2761 tggcaaggta atgtcattag tcacaatagt gcatttgaac attatttcac tgctgattac
     2821 tacaaaaatg caatgttgcc attagaaaac agtgaatcac cctttaaaga tgttttttct
     2881 aatacgcatg aagtcacagc agagacgaaa gaaaaccgaa caatatacac acaggtattt
     2941 gaaattgata atggcatcga gaaaagatgc attaatcact ggcatacatt atgtaatctg
     3001 ccagccagcg aacatgctgt ttatatttgt ggttggcaag atattaccga gacgcgtgat
     3061 ttaattcatg cactcgaagt agagagaaat aaagcgatca atgcaactgt cgcaaaaagt
     3121 cagtttctgg caacaatgag tcacgaaata agaacaccaa taagctccat tatgggcttc
     3181 ctggaacttc tgtcgggttc tggtcttagc aaggagcaac gggtggaggc gatttcacta
     3241 gcctacgcca ccggacaatc actcctcggc ttaattggtg aaatccttga tgtcgacaaa
     3301 attgaatcgg gtaactatca acttcaacca caatgggtcg atatccctac tttagtccag
     3361 aacacttgtc actctttcgg tgcgattgct gcaagcaaat cgatcgcatt aagttgcagc
     3421 agtacatttc ctgatcatta cctggttaag atcgaccctc aggcgtttaa gcaggtctta
     3481 tcaaatttac tgagtaatgc tctcaaattt accaccgagg gggcagtaaa aattacgacc
     3541 tccctggttc acattgatga caaccacgct gtaatcaaaa tgacgattat ggattctgga
     3601 agtggattat cacaggaaga acaacaacaa ctgtttaaac gttatagcca aacaagtgca
     3661 ggtcgtcagc aaacaggttc tggtttaggc ttaatgatct gcaaagaatt aattaaaaac
     3721 atgcagggtg atttgtcatt agaaagtcat ccaggcatag gaacaacatt tacgatcaca
     3781 atcccggtag aaattatcca acaagtggcg gctgtcgagg caaaagcaga acaacccatc
     3841 acactacctg aaaagttgag catattaatc gcggatgatc atccgaccaa caggctatta
     3901 ctcaaacgcc agctaaatct attaggatat gatgttgatg aagccactga tggtgtgcaa
     3961 gcgctacaca aagtcagtat gcaacattat gatctgctta ttactgacgt taatatgccg
     4021 aatgtggatg gttttgagtt gactcgcaaa ctccgtgagc aaaattcgtc cttacccatc
     4081 tgggggctta cagccaacgc acaggctaac gaacgtgaaa aagggttaaa ttgcggcatg
     4141 aacttatgtt tgttcaaacc gttgaccctg gatgtactga aaacacattt aagtcagtta
     4201 caccaggttg cgcatattgt acctcagtat cgccaccttg atatcgaggc cctgaaaaat
     4261 aatacggcga acgatctaca actgatgcag gagattctca tgactttcca gcatgaaaca
     4321 cataaagatt tacccgctgc gtttcatgca ctagaagctg gcgataatag aactttccat
     4381 cagtgtattc atcgcatcca cggtgcggct aacatcctga atttgcaaaa gttgattaat
     4441 attagccatc agttagaaat aacacctgtt tcagatgaca gtaagcctga aattcttcag
     4501 ttgctaaact ctgtaaaaga acacattgca gagctagacc aggaaattgc tgttttctgt
     4561 caacaaaata actaaatagt ggctcccaca atgttcaaat gtgggagcta tttaccagca
     4621 catcttacat ttatgatgag aactcctggc gaatttgctc cccatgctgg tcgagcgttg
     4681 ccgctcccgg cataacatgc gggtccgcgc agccactgat ttttatcgga ttacccggca
     4741 tcattattcc cccggcttca atcaacatat ttctcgcctg agtttgtggc aaattaatgg
     4801 cctcagccac acttaataac ggcgcgacgg gcacaccaac ttcatgtatt ctggctaacc
     4861 aaacttcagc tgcctgtgtt tttaacgtcc gctcaatata ttgtttaaga atagtctggt
     4921 tttgtacgcg taaaatattg ctgctaaatc tgggatcatt aaccagttcc gtaagctcca
     4981 gtgcctggca taacgcagag aaaagcttgt cattaccaca acaaatcgta atcggcttat
     5041 cctgagtatc gaaaacatca aaaggtgcca tgtaggggtg gcgatttccc aggcgttgtg
     5101 gtgacttccc tgtcgcaata tatgccatca gaccatgctc cagaaaactc agcgtggcat
     5161 caaacatcgc tatatcgaca tgcgcccctc tttggctctt ttcgcggcca taaagtgcac
     5221 tcactattcc gctgaataaa taaacaccgc cgcatagatc cgcaagagag gtaccaacac
     5281 gcactggcgg agcatcaggg taccctgttt ccatcattat cccgctcatt gcctgaatga
     5341 tggtatcgta ggcaggagca tcttttagcg gaccggtatg tccgaaacct gacgatgaag
     5401 catatatgag gcgcgggttg atttcttgta acctttccca tgaaagcccc agtttttcca
     5461 ttgtacctgg gcgaaaattc tcagctaata catcagcttg tttaagcata tttataaata
     5521 tacttttatc gtgatcattc tttaaatcaa gaaccacact ctctttacca tgattaataa
     5581 aactgtaata gagtgactgt ccatccacat agggaccaaa tgtgcgggta tcatcaccat
     5641 gacccggcgg ctcaacttta attacccttg cgcccatatt acaaagaagt tgagttccga
     5701 aaggtccatt aaggacatgt gtcatatcga taactaataa gccttcaaac ggccctttgc
     5761 tttcattatt tgtcatttgc ttatgccttg aaaaaagccg aaggtgaaaa gacacttcgg
     5821 catttatgta aaatcaataa catgattaat tatgaaacca gtcgactgac ataaatccac
     5881 aagggagccg tgacgacaaa acccagtacg ctcacagcca atgacgctgt accagtgcgg
     5941 gtgtagacat taaaccggct ggcaataatg attccggaga atgccggcgg taatgcgcct
     6001 gccagtacca tcatctgcag atgttcgctg ttcaaatgac atgccatacc aacaagaagc
     6061 agcgccagtg gcatcagaat cagcttcagg aaggtgttat aagcaatttc ggcactgaac
     6121 tcgaatttat gtgcagccag agtcaacccc gcagcgaata ccgctacccc tgagttagcc
     6181 ttcgcaatca gattaaaggt tgggtcccat gctgccggaa tttttacccc aaccaacacc
     6241 aggatcgttg ccagaacagg tgcccataca actggctcct ttgccgcaga aattaatgcg
     6301 ctcagattac tattcttctt accatccgct cctgaggaag gattcagcaa atacagacca
     6361 ataggaatag taattgcgtt aacaataata gaaataattg ctaccactaa acctgttgat
     6421 acggaatcac cataaatagg atcgagaact gcaaacccca ggaatccaat ggtaggtgaa
     6481 cctgcaatta atgcacatac agctgcttct gcatgggtac gtttaaaaaa tttgtagcaa
     6541 ccaaaccagg agaaaaagaa acatccgaca ataaccacga gtgatacaag ggtcagacga
     6601 gtgtccgcaa aaatcatttc cctgtttgcc cgggtaatag aaacaaatag ggctgcagga
     6661 agcgcgtagt ttaataccag tttattaaat gcccgggctt gatcttctga aaaagtttct
     6721 cgtctgccgc taaaataacc caacagcatg attacgataa taggcaataa atcgccaata
     6781 aaaaatgtta gcataatagt atttccgata agggtcccct caccatcgca taatgagttg
     6841 gcaagaggag acgatgtcag tatttaatta ccagcgactt gttttgggtt aagtttggta
     6901 atatggccac tttcagttcc tgctgcaggg tcgatgacca cattaataat ggtcggtttg
     6961 cgcgactgaa taccggtggt taaagcatga cgaagttcat ctgtcgtggt gacgttatag
     7021 ccaacgccac gaaacgcatc cattaatttg tcataccttg catggtgcaa cagatctgtt
     7081 ggtgatggtg caccagcgcc actgagatca acaccgtctc ctctgtagat gccgccatta
     7141 ttaaaaataa cgatcgttac cggcaggtta tatcgacaaa tcgtttcaat ttccatccca
     7201 ctgaaaccaa aagcactatc accttcaatg gcgacaaccg gagaaccaga ggtcacgcta
     7261 gcaccgatgg catagcccat accgatgccc atgacacccc aggtaccaca atccagacga
     7321 cgacgtggtt tatacatatc aataatattt cgtgcattat ccagggtatt tgcaccttca
     7381 ttaactaaat aaatatcctg gttctcacgc aatacatcgc gcacagcact taatgcatta
     7441 aagtaattta atggttgggt atctgtactt aatttttcat gcattttttg tgcattttgc
     7501 tgcttgtgga tatttaaaat atcgcgccat accagtggag tcgtaaatgt gttttgtttc
     7561 agctctgcca gcataccttg catactggat gcaatatcgc caacgactgg cacagcaatg
     7621 gggcggttgc tgtcaatttc ctgcggttca atatccagtt gaataaactg tgtatctgcc
     7681 gcccatcctt ttttaccgtg tgccagtaac caattcagtc gtgcaccaac aagcatgaca
     7741 acgtcagcat ttgccagggc aaacgaacgc gcagctgccg cagaaagtgg atgcgtatct
     7801 tcgaggatcc ctttcgccat agacattggc aggaatggaa tctgggcact ttcaataaat
     7861 tcacgaagct gttcgtcagc ttgtgaatac gccgcgcctt tgccaaggat aattaatggc
     7921 cgttcagctt ttgctaaaag cgaaattgcg ctagtgactg acttcgggca tggcaataat
     7981 gctggcgacg gattttcaac ttttacaatc gtggttaacg cttcgtcttt ttccatcgtc
     8041 gcggccagga catttgctgg caaatcaaga taaactccgc cagggcgacc cgatactgag
     8101 acccggatag cgcgtgccaa tgcaatgcca agatcctgcg gctgattaac gcgaaatgct
     8161 gctttggcat acggttttgc cgcattcatt tggtccagct cttcataatc accttgctgt
     8221 aggtcgacga tcgcgcggtc gctggagccg ctaatcatga tcatcggaaa accatttacc
     8281 gttgcgttgg ccaatgcggt caaaccattg aggaatcctg gcgcagacac tgtcaggcag
     8341 atccctggtt tttgggtaag aaaaccgctt gccgcagcgg catagcctgc cgactgctca
     8401 tgacgaaaac caatataacg aatgccttcc gcctgggcat ggcgcgccat atccgtcaca
     8461 ggaataccta caacaccata aatagtgtca atattattct gttttaatgc ttcaacgatg
     8521 atatgcatac catatgtcat ttgaagttga tctgacatga ttaataacct cgaaattttt
     8581 ataaattggg agccagaagc ccccgttaat atcagatggc gtggttttgc ttcattgcag
     8641 caatttcatc gtcgctataa cccagctcct gcaataccgc agcggtatgt tcacctaata
     8701 gaggcgcagc tttaatatcc ggcgtaaagg cagagaattt cattggacaa ccaactgtca
     8761 gatattttcc acgcaatggt tgttccactt cgacaacact gccgctttgg cgtaaagagg
     8821 gatcaagtga aatttctttc atacttaaaa ccggtgcaca aggaatatca aactgagtca
     8881 aataagccac tgcttcatgt ttatcaatag tgacagtgta tttttcgatt tcagcaaaaa
     8941 tatcgaaaat atgtggctgt cgggcatggg ctgtactgta tgccggatcg gtaatccaat
     9001 ctggtttgcc gatggcttta caggtgtttt cccagttttg ctcctgaata gtgaaataaa
     9061 tataggcgtt aggatcagtt tcccagcctt tacatttcag gatccagcca ggctgaccgc
     9121 caccacccgc attacctccg cggggaactg catcaccaaa tgtgccattc ggatactgcg
     9181 ggtattcttc cagataaccc aatttatcga gacgctgctg gtcgcgtaat ttcacgcggc
     9241 aaaggttcaa tacggcatcc tgcattgaca tggtgactcg ttgcccacgc cccgtttttt
     9301 cgcgatgcag caaagcagca agtaaaccga tcagtaaatg cattccggtg ttgctgtcac
     9361 ccaacgctgc agcgcttacc agcggcggac cgtcccaaaa acccgtagtg gatgccgcgc
     9421 cacccgctgc ctgagcaacg ttttcatagg cttttacatt cacataaggc gaacactcat
     9481 caaacccttt gatcgaacca aaaatcagac gtggattgat ttcttgaata tgctcccagg
     9541 tgaagcccat gtgatcaatg gcccctggat gaaagttctc gactaagata tcagcttcgc
     9601 ggatcagctt ttccattacc tctttgcctt ccgctgtttt ggtatttaac tcaatagaac
     9661 gtttgttact gttaagcatg gtgaagtaaa gcgcatcgat atcaggaata tctcgcagct
     9721 ggtgacgcgt tacgtcacca acgccggggc gttcaatttt aatgacgtca gcgccaaacc
     9781 aggccagcat ttgagtacaa gatgggccag attgcacacc ggtgaaatcg agaactttaa
     9841 ttccttgaag tggagttgac atagcaatac ctattagtta attaaatatg tgttaattta
     9901 gcgtttattt aaggcataac gccctctata aataatccgc aaaatatcgt catagcggca
     9961 ctcgatattt ttaaattcct gtatcaagtg ctgaaaatca tttcatcgct acttccaatt
    10021 cctttctgca tggtaagtag tgaggacgaa aagaagaata tgataaatat tggttcgtgg
    10081 taatcagaga gagtcgcgac atttgtgatg ttattcttac aactgtaaga agcgccggtg
    10141 atttacaagt aagggtatga ttacgctttt tagagcgtac cataaaatat aacatattaa
    10201 atgatttgtg atgacacggg attattgcgg tttactttga ggaatataaa atgcgagttt
    10261 atagaacagc gagcgccaaa taattttatg accacaacct gtcgtattgt ggtcataaaa
    10321 aattgtcggc tggagggagt taaatccttt acctcaatat actgcctgtc agcattagtt
    10381 cacaaacagt gcttcgctat cgacgatgat tccatcttcc gcacctttaa gcgccaggtt
    10441 tgcctcgtag tattgctttt tatcaagcag tttctgcgca tcagcaagtg cattacgagt
    10501 ctgtttaagc ggcatcagat attggttttc cattacacca acaccggcca ggcgaagctc
    10561 ttccattgcg ccctttttat cccctttcgc cattttttca ttggcaattt ttatcgcggc
    10621 ttctttttcg ggtgtcgcta cataactctc agaaattccg acagatgcat taatgacaat
    10681 atattggtca tcattgacat tggtcttttt acctggctta gcgaactttg cccattcggt
    10741 actgtcatct gacaacaaag ctgaagcttc attggtcagt tcttttgcct tttccggatc
    10801 gccatgaaac agagccaggc gcgctacctg cacgtcacgc atcgcatata aaccttgttc
    10861 agagatacgc tctgctgcag ctgccgtttt ctgggtttgc tgaacttgct gagtttgctg
    10921 ttgtgccgct accggagcat tatcagcagc ccatacagtt gaagatgcca gaatagctgt
    10981 gaccatcgta gccataatta aacgtttcat acttacctca ttctttaaca gttttagaaa
    11041 acgccgccat catattgggt gagcatgaaa tgagaaatca gagagcaacg gtcattttga
    11101 aagggttatc ttacagttgt agttattatc gcaagaagta t
//
LOCUS       DAZFAB010000132        10995 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZFAB010000132 DAZFAB010000000
VERSION     DAZFAB010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10995
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.132"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            218..868
                     /gene="csgD"
                     /locus_tag="ACHJWA_004127"
     CDS             218..868
                     /gene="csgD"
                     /locus_tag="ACHJWA_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFO4310615.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            873..1262
                     /gene="csgE"
                     /locus_tag="ACHJWA_004128"
     CDS             873..1262
                     /gene="csgE"
                     /locus_tag="ACHJWA_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFO4310616.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            1287..1703
                     /gene="csgF"
                     /locus_tag="ACHJWA_004129"
     CDS             1287..1703
                     /gene="csgF"
                     /locus_tag="ACHJWA_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFO4310617.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1730..2563
                     /gene="csgG"
                     /locus_tag="ACHJWA_004130"
     CDS             1730..2563
                     /gene="csgG"
                     /locus_tag="ACHJWA_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFO4310618.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2627..3118)
                     /locus_tag="ACHJWA_004131"
     CDS             complement(2627..3118)
                     /locus_tag="ACHJWA_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFO4310619.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILSYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLIFGYAMKNSGLWLAARSAKTALREQEIK
                     NKA"
     gene            complement(3220..3774)
                     /gene="ycdY"
                     /locus_tag="ACHJWA_004132"
     CDS             complement(3220..3774)
                     /gene="ycdY"
                     /locus_tag="ACHJWA_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFO4310620.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3798..4535)
                     /gene="ycdX"
                     /locus_tag="ACHJWA_004133"
     CDS             complement(3798..4535)
                     /gene="ycdX"
                     /locus_tag="ACHJWA_004133"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFO4310621.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4590..5528)
                     /gene="ghrA"
                     /locus_tag="ACHJWA_004134"
     CDS             complement(4590..5528)
                     /gene="ghrA"
                     /locus_tag="ACHJWA_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFO4310622.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALA
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLKPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGWEELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            5762..5849
                     /locus_tag="ACHJWA_004135"
     tRNA            5762..5849
                     /locus_tag="ACHJWA_004135"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5796..5798,aa:Ser,seq:gga)
     gene            complement(5999..6841)
                     /locus_tag="ACHJWA_004136"
     CDS             complement(5999..6841)
                     /locus_tag="ACHJWA_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO4310623.1"
                     /translation="MSDITISRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISVLTESIGGKTAPDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEVSEANILSTFEQLHQNKDEVFERGVINVFRGLS
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGHQVDRLADLERMLHLFSGKPIPDNR
                     ENITLRLDDHIQSVQGKEDYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKHYPD
                     MLAV"
     gene            complement(6926..7123)
                     /locus_tag="ACHJWA_004137"
     CDS             complement(6926..7123)
                     /locus_tag="ACHJWA_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO4310624.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(7135..7626)
                     /locus_tag="ACHJWA_004138"
     CDS             complement(7135..7626)
                     /locus_tag="ACHJWA_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO4310625.1"
                     /translation="MMKLALTLEADSVNVQALNMGRIVVDVDGIELAELINMVGDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGNGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(7623..7997)
                     /locus_tag="ACHJWA_004139"
     CDS             complement(7623..7997)
                     /locus_tag="ACHJWA_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO4310626.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSACPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(8044..8418)
                     /locus_tag="ACHJWA_004140"
     CDS             complement(8044..8418)
                     /locus_tag="ACHJWA_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO4310627.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLNAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            complement(8581..8802)
                     /locus_tag="ACHJWA_004141"
     CDS             complement(8581..8802)
                     /locus_tag="ACHJWA_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO4310628.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(8865..9341)
                     /locus_tag="ACHJWA_004142"
     CDS             complement(8865..9341)
                     /locus_tag="ACHJWA_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFO4310629.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(9357..9842)
                     /locus_tag="ACHJWA_004143"
     CDS             complement(9357..9842)
                     /locus_tag="ACHJWA_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO4310630.1"
                     /translation="MTTVSHNSTTPSVSVTTASGNNPPQLVATPVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(9933..10751)
                     /locus_tag="ACHJWA_004144"
     CDS             complement(9933..10751)
                     /locus_tag="ACHJWA_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO4310631.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMRHVPSIFGENRHTSRSEHY
                     AYIPTITVLENLQQEGFQPFFACQTRVRDQSRREYTKHMLRLRRAGQITGQHVPEIIL
                     LNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
BASE COUNT         2776 a         2737 c         2739 g         2743 t
ORIGIN      
        1 aatataacca gttacactta gttacatgtt taacacttga tttaagattt gtaatggcta
       61 gattgaaatc agatgtaatc cattagtttt atattttacc catttagggc tgatttatta
      121 ctacacacag cagtgcaaca tctgtcagta cttctggtgc ttctatttta gaggcagctg
      181 tcaggtgtgc gatcaataaa aaaagcgggg tttcatcatg tttaatgaag tccatagtat
      241 tcatggtcat acattattgt tgatcactaa accttctttg caggcgacag ctctcttgca
      301 gcaccttaaa caatcgctgg caattacagg aaaattacat aatattcaac gttctctgga
      361 cgatatctct tcaggctcta ttattcttct ggatatgatg gaagcggata aaaagcttat
      421 ccattattgg caggatactt tgagcaggaa aaacaacaat atcaaaatat tgttgctaaa
      481 tacgcctgaa gattacccgt accgcgacat tgaaaactgg cctcatatca acggcgtttt
      541 ttatgccatg gaggatcaag aacgcgttgt caatggattg caaggcgtcc tgcgcggcga
      601 atgctacttt acgcaaaagc ttgccagtta cctgattaca cattctggta actatcgtta
      661 taacagcacg gaatcagccc tccttactca tcgggaaaaa gagatcctga ataagctgcg
      721 tatcggcgcg tctaataacg agatcgctcg ttcgttgttc atcagcgaaa atacggttaa
      781 aacgcatctt tataatcttt tcaagaagat agccgtaaaa aaccggacac aagcggtttc
      841 ctgggcaaac gataacctca ggcgataaag ccatgaaacg ttatttacgc tggattgtgg
      901 cggcagaatt tctgttcgcc gcagggaatc ttcacgccgt tgaggtagaa gtcccgggat
      961 tgttaactga ccatactgtt tcatctattg gccatgattt ttaccgagcc tttagtgata
     1021 aatgggaaag tgactatacg ggtaacttaa cgattaatga aaggcccagt gcacgatggg
     1081 gaagctggat cacaataacg gtcaatcagg acgttatttt ccagactttt ttattcccgt
     1141 tgaaaagaga cttcgaaaaa actgtcgtct ttgcactgat tcaaactgaa gaagcactaa
     1201 atcgtcgcca gataaatcag gcgttattaa gtacgggcga tttggcgcat gatgaattct
     1261 aaataaaaaa ttgttcggag gctgcaatgc gtgtcaaaca tgcagtagtt ctactcatgc
     1321 ttatttcgcc attaagttgg gctggaacca tgactttcca gttccgtaat ccaaactttg
     1381 gtggtaaccc aaataatggc gcttttttat taaatagcgc tcaggcccaa aactcttata
     1441 aagatccgag ctataacgat gactttggta ttgaaacacc ctcagcgtta gataacttta
     1501 ctcaggccat ccagtcacaa attttaggtg ggctactgtc gaatattaat accggtaaac
     1561 cgggccgcat ggtgaccaac gattatattg ttgatattgc taaccgcgat ggtcaattgc
     1621 agttgaacgt gacagatcgt aaaaccggac aaacctcgac catccaggtt tcgggtttac
     1681 aaaataactc aaccgatttt taagccccag cttcataagg aaaataatca tgcagcgctt
     1741 atttcttttg gttgccgtca tgttactgag cggatgctta accgccccgc ctaaagaagc
     1801 cgccagaccg acattaatgc ctcgtgctca gagctacaaa gatttgaccc atctgccagc
     1861 gccgacgggt aagatctttg tttcggtata caacattcag gacgaaaccg ggcaatttaa
     1921 accctacccg gcaagtaact tctccactgc tgttccgcaa agcgccacgg cgatgctggt
     1981 cacggcattg aaagattctc gctggtttat accgctggag cgccagggct tacaaaacct
     2041 gcttaatgag cgcaagatta ttcgtgcggc acaagaaaac ggcacggttg ccattaataa
     2101 ccgaatcccg ctgcaatctt taactgcggc aaatatcatg gttgaaggtt cgattatcgg
     2161 ttatgaaagc aatgtcaaat ctggcggggt tggggcaaga tattttggga tcggtgccga
     2221 cacgcaatac cagctcgatc agattgccgt gaacctgcgc gtcgtcaatg tgagtaccgg
     2281 tgagatcctc tcctcggtga acaccagtaa gacgatactt tcctatgaag ttcaggccgg
     2341 ggttttccgc tttattgact accagcgctt gctggaaggg gaagtgggtt acacctcgaa
     2401 cgaacccgtt atgctgtgcc tgatgtcggc tatcgaaaca ggggtcattt tcctgattaa
     2461 tgatggtatt gaccgtggtc tgtgggattt gcagaacaaa gcagaacggc agaatgacat
     2521 tctggtgaaa taccgccata tgtcggttcc accggaatcc tgacagataa aaatgccgga
     2581 attaataatc cggcatttac tgtttaagcg aaagcgtgag tgcccctcac gctttatttt
     2641 tgatttcctg ctcacggaga gccgtctttg cactacgcgc cgccagccac aggccgctgt
     2701 ttttcattgc gtaaccaaat atcagcccaa gcgccagcga aggtaacacc agtttccagt
     2761 caccctgccc ggcaaacgtc gcgcatgcgc ctataaacgt ccccggaaca aatgaaagca
     2821 gcagctgttt ggcctgaata cacatcagaa acgcgacaat accggttatg acataactga
     2881 gaatttccag atgtggcgcc agtgcactac cgtaaataat gaccatcgcc cacaccacgc
     2941 cactaagcag agttgcagcg gagatcgcca gccctttcag cccaccttgc gggcaggcaa
     3001 agtaggccgt acagcccagg aagcccgccc agcttagtaa gcccagggaa acagccaccc
     3061 atccccagat accggagaga atgcctgttg tgattgcaat agagagaagt atgttcatgc
     3121 cgcgcatgat agcagaaaac gcggccatga atgagatcga gcacacattt tatgcaacag
     3181 caaattatct gttgcagtta tgagtgacgc atatcacatt tactcttcag aatcttcttc
     3241 cagctcgtcc cacattgcac taatggcatc gcgggttagc ggtgccatgg tgcgccagaa
     3301 aggcgtggtt gcatgggcct ccactttgcc aaggaacgca ccacaccagg gtaacagata
     3361 ctcactgaac agtgtttcca gtgcttcgct ctcatcttcc gttgactgat cttccagcca
     3421 ggaagccgca agcagcaatg tgccgatgtg atcggcaggc gtatccgcta atggcatccc
     3481 tcgttcagaa agaaaagcgc gcacttccgc ttccgttgcg tcctcaaccc atgcgctacg
     3541 atatggcggc acggcacatt catcgccgat aaacaacgca ttgtaatcgg cagagacttg
     3601 cgtcatatca cagcttttct gcaaacgtgt cagcaactca tcctgctcca gtggccagtt
     3661 cgcagccagt ttcccctcac gaatcagggt aaacagcggc accagtaaag gatcttgcgg
     3721 ttggcggtaa tagagcgaac ccagcacacg acagaggata gaaaactcgt tcattaatat
     3781 atttccatta ctagtaatta aaggtctgca aattccgcaa tcggtgccat accgcgagat
     3841 tcaaggaagt tcagtaagcg gcgcggagaa acattcaaaa tgcgctctgg cggaaaatct
     3901 accgcgtcga ggattttaag acactcttca aattccccca tggtaaacgc ggtgtgagaa
     3961 tccgagccta atgccaccca acctccagca tcacgtaccg ctgcggctac cgcacgacag
     4021 ttgtcttcac tgcccttacg tgagtgtaaa aatgaggaat tattgatttc cagcgccacc
     4081 tgatgtttcg cggctgcttc agcaacggct ttaacatcta tctcatattt cggatttccg
     4141 ggatggctta ttatatgcac attgccgctg gcgatagtgg cgatcatcgc ttgtgtattg
     4201 gtcgctttgt catgtggtgc aaaaaccggc tcatgaaaac cggcaataat cagatccagc
     4261 gagtcaaaca ttttaccgct gcaatcaatt tcaccatcaa catttttaat gttagcttcg
     4321 atgccgcgca ggatccctac cccatcaacc actcgcggcc agatacgcat gttaatgaag
     4381 tgccagtgat gcggcgcatc ttccatgtcc gggccatgat cggtaatcgc aaaaagttta
     4441 atgccctttt gtttggcctg ggcaatgtaa tcactcaatg tgctatatgc atgtgtgctg
     4501 gcaacggtat gcatatgaag gtcgacggga tacataactc tctcctgtat tcaattcccg
     4561 ccaaggatag caggaatcct gatgctttat tagtagccgc gtgcgcggtc gacttgcccg
     4621 cagaccctct cccctttttc gagctgggca atagtgcgag aaatgtactc cacagcttca
     4681 gcgggacggg taatcgcggc gacatgtggt gttatcgtca cgcgtggatg ttgccagagc
     4741 ggactttcag gcggtaaggg ttcacgatta aaaacatcca acattgcgcc tttaactttg
     4801 ccgctatcca gcgccgcgag caggtcatct tccacgacat gaacaccacg cgccaggttg
     4861 aggagatacg cgccatccgg taatttttcg agtaattgtt gattaataat gccgacggtt
     4921 tcaggggtat tcggtaacaa attaatcaat acccgacatt ggctcagaaa tgcagacagt
     4981 tcttcccatc cggcaaagct ttgcacgcca ggccacgatt tacgggttcg actccagcaa
     5041 cgcagcggaa agcgccaggt ttgcagactc tgagcaactt tactgcccag tacgcctgcg
     5101 cccaaaatgc cgatggtaaa atcttcccga tgatattcag gcagcggttg ccaatgcgaa
     5161 ctattttgct ggatgcgata atcgtcaaaa cgtcgaaacc aatgcagcac ctgactgaca
     5221 gcatattcct gcatttgctc gcccataccg gtatcttcca ggcgaaaaag tggaacagaa
     5281 ggcttcagca tttcagggtg tgcctgtagc ttgctcaaaa tagaatcaac accggccccg
     5341 agtgcgaaca ccgctttaag atcgcgccct gccagcattt caacaggagg atgccaggct
     5401 aaagcataat cagcagagtc attatctccg cttttccatg ctctgactct tgcctgagga
     5461 atagctttgc gcagtgcctc tatccaccat tgggtatcga acgttgggtg ataaaagatg
     5521 atatccatac tgactcccga aaagcgttgt gcgaaattta ttcgcactta tcgttatgat
     5581 ctacaaagac caccagcata acaaatccgt ggtcggtggc caaaaaagca gattttgctt
     5641 attaaaactg catattgatt gaagtttgaa taaacgcgcg attttttaaa aaagtttgtt
     5701 gacctcaggt catgatttcc ctaaattagc gcccgttcca gcaagacagg aacgacaatt
     5761 tggtgaggtg tccgagtggc tgaaggagca cgcctggaaa gtgtgtatac ggcaacgtat
     5821 cgggggttcg aatccccccc tcaccgccag attataagcg catgttattg tttaacatgc
     5881 gcttttttat ttatccttcc ggacagtatg ccatcgggta tgccatctga aaccggttta
     5941 tatgccccgc gatcatatct tatcggacaa tttccgatct ttgagcatac tggcaaagct
     6001 atactgccag catgtcagga tagtgttttg cgataatatc attcagtctg tcaaccagtt
     6061 ctggtttcct gaacgtgatg tgtgcggagc ccttcttaaa gtatctgatg ctgaacatct
     6121 catcttcata gtcctcttta ccctgaacag actggatgtg gtcatccaga cgaagggtga
     6181 tgttttcccg gttgtcgggg atcggtttgc cgctgaacag gtgcagcatt ctttccaggt
     6241 cggcaagtcg atctacctgg tgcccggtga taagatgaaa tccccaccgg tcccatctca
     6301 ccagattgtt gacgataatt ttactgccaa atttacaggg gcaattagtt ttgtaattcc
     6361 agctcaatcc cctgaagacg ttgatcaccc ctcgctcaaa cacttcatct ttgttctggt
     6421 gcagttgttc aaatgtgctc agaatgttcg cttcactgac ttccggaaaa ttatcgtact
     6481 ccagtgaccg gtaccatgta tcacgggcct gtgcatccat taaggaaagc atccctgaac
     6541 gttgcatcag gtcacgccag atttcgcggt caagattccg ggtaatggct ttcattgctg
     6601 tttcgggttt ctccatcagc cagcagccgc agcgaaaatc ctgtttcatg gcccagtccg
     6661 gggcagtttt acctccgatg ctttccgtca gcaccgagat ttcagccagt tgtcggataa
     6721 gtgtgtcgat ttgcaacagt gtactctttc gtacagccag aatgtggcga atgctggttg
     6781 agcagataac gtccgtatgc ccgttgacca cttccggacg ggagattgtg atatctgaca
     6841 taatcgatat tcctttttgc agataaatga aaacgctagc cctgtgcagg ctggcgtcag
     6901 aggaatgaag tctggtgagt gagcttcacc ggttctggtg cagaagctgc agatgacgca
     6961 gggtacggac atcctcccgg gcctgttcga tacagggcaa cagtgctgct gaatccgttt
     7021 tatcctcatc gttgtcaaag ataattcccg atccgcagtc gatattgtcc tgcagccacg
     7081 caatcagaat atccagcgct gtttccgtgg ttaatgattt catgttgttc ctggttacca
     7141 gtcgaaagtg ggtaaacccg gcaggcattc agcgctggca tccagatgaa tgagactgac
     7201 gccataacgc cggatgagtg tgacgaccag acgacggaac gtttttgaca gacccaggcg
     7261 tttaagccgc agaaccgggt acgaccacgc atcggtacgc aacagatagc cgttaccggt
     7321 aaaatgaatc cattctgatt caccgaagtc actggtctgg tgtgacagcg agtacagcca
     7381 ggcgttgtcc gtttccgtga tatgtgcggt actgcagcgt atgccggtaa gggtagcaaa
     7441 tggtggcgtg cattctgctg aggtctggtc agattcatca acaacacgaa gggagtagcc
     7501 gttatcgccg accatgttaa tcagttcagc gagctcaata ccatcgacgt caacgacaat
     7561 gcgccccatg ttcagtgcct gtacgttaac gctgtcggct tccagtgtca gggccagttt
     7621 catcatttcg cctccggata cttacccagg gtaatgttat ttaccgttct gtaattgtcg
     7681 cgggtcatca ggccggtcgc ccggcgagcc cggaggatat caatactgtt tattaactga
     7741 gagcggggac aggcgctgaa tcccggctgg tcggtacgca ccagcgcgta tttttccacg
     7801 agaaaattaa ctgcatcaca cagtgaaatg cctgcctcaa tatgctgctc aatcacacgt
     7861 tcatcggcaa acggtgtgtc attcagtgtg aggccataat gctggtccag cagtcggatg
     7921 agcagtgtct gccagatggt gacgggagac gggcagcgag atacctcccg tacatgtgtg
     7981 tcggataatg ttttcatctt aagttctctg taactggtag tgctaaaggt gagagcttcg
     8041 gggttatgag gtggttgcgg gtgctggtgt cggataaaca gccagataaa cgtaaccaca
     8101 ggagccgagg gtgtcggctt cgcaggtcag cccttttgca ttcagcgtga cggtatgctg
     8161 atatcgggga ttcagttcac cgctggtgag catgagttcc agttgtttca tcagcagcgg
     8221 aaaggcctgg tccagatggt atgcatctgc gtcgctgaag cggcctctga taccggcgcg
     8281 gtcggcaagg taatgcaggc ggttaccctc ttgcaccaga cgagcgccaa aacagggcct
     8341 cactgtgcag ggaagccccc accaggggcg gtcgtgattg tcatcgggat gagttatccc
     8401 ggagagtttg tctgacacga taaaatcctc tctgaaaagt ggttaagaat ggtttaacga
     8461 tgacaggtca gccggcgcgc agaccggttt cggtgaagat gtatgcattg atgagaatgg
     8521 cctcatccac agcggcgtct gaggtcatct ggtcgtactc gtttcccggg tgacggaaca
     8581 tcagttcagg gtgacactca tcagccggac atacgggcca aaactgtcct tacggcgttc
     8641 tgcaaacacg gcaagaacgc cgggaatatc ctgcacttca cgaccagtat acgcttcagc
     8701 actgccgtgc cagcgatact taccggtaca gaacggaaaa agacgggatg caggatgctg
     8761 ttggtgaata cgcatggctt caccacgggt gatgattctc atgatgtgat acctctgaag
     8821 acagaagata aaagtgaaaa caggtgtgat gtggttgtga cgggttaaag cagaccatgt
     8881 tcggcaaagg agaaaacctg gttgccaccg actatcagat ggtccggcac ccggatatcc
     8941 accaggccca gtgcctgtac cagacgttcg gtgataagcc ggtctgcctt actgggtgtg
     9001 acttcaccgg acgggtgatt gtgcgccagc accacggcag cggcattgtg gtacagggcg
     9061 cgtttaatca cttcccgggg atgaacttcc gtacggttga tggtgccggt gaagagggtt
     9121 tcaccggcaa tcagctgatt ctggttgttc agatacagca cccggaactc ttcccgctcc
     9181 agtcccgcca tgttcagaat cagccattcc cgtgccgcac gggtggaggt gaaggccacg
     9241 ccgggttcat gaagatggcg gtccagggtt tgcagggccc gcagaatgag actgcgctcg
     9301 ccgggcgtca tctctccggg cagaaaggaa agctgctgca ttgtccctgc ctccattcag
     9361 tcaatgatgc gcataatggc gctgcattcc ggatgctgca gggcgtaatc ccgcagccgg
     9421 taatagtgga ccgtcatggc ataacactcc gtacgacagg catgatgact gtacgtcatc
     9481 agacaggcag caataccggc ggcttccggg ctcatttcag cgcggttgcc gttcatggca
     9541 ttgaacagta cccatgtttc gtcatcatcg tcatccggtt cgggtgccat aaatgccccg
     9601 ccgttgttca gggtgtacag attccagata ccaccgcagt agtcttcgca aagacggtcc
     9661 atccagccga agacacgggg ctccagggtc acccactgtg gaatgaggcc aaaatgctgc
     9721 ggccagaagc tgatgcgctg ttcatcaggg acgggagtgg caaccagctg aggtgggtta
     9781 ttccctgatg cagtggttac ggaaacagaa ggtgtggtgg aattatgcga aacggttgtc
     9841 atgaggttat tccttataaa aagtaaatga atggaagaag ccccggggca ggaacagatg
     9901 tgagtcagga ctgcactttc agtgaaacgg catcagcgca tgctctccag cagcgtttct
     9961 gccatcaccc acaatgcgcg attgagctta atgtcggtat caatgctgtg aatggcccgg
    10021 gtatggatac gttttccttt tgcactgcga ccggaaatcc cgcctttcag catattctcc
    10081 tggatggtct gataagcact ccacaggtcc ttaccgtaat cctcccggcg tcgtggtgtc
    10141 agaatgtcgg cggtggtgac gggctgatgt tcgtcaccat aacggtaagt cagtgccgcc
    10201 tgtgccagcg cctggcgtgc cggtggcggc aggaccagcg actgcatggc atcacgcttc
    10261 tcctctatcc ggtcaaaaac gcccaccacc tcgtaagccc cttcaatgac tctgtccacc
    10321 acatttcccc ggtgtggaac acgcaattct cccagagact gaccgcagac cagtccattg
    10381 gtacaaatgg cacgaaaata tccgggtaac atctggtagc tggatgaacc gtcatgggag
    10441 ttgagcagaa taatttcagg gacatgctga ccggttatct gtccggcccg ccgcagacgc
    10501 agcatgtgtt ttgtgtattc ccggcggctc tggtcgcgca cgcgggtctg gcaggcaaag
    10561 aacggctgaa agccttcctg ctgcagattt tccaggacgg tgatagtggg aatgtacgca
    10621 tagtgttcac tgcgagaggt atgtcggttt tccccaaaaa tactcggtac atggcgcatc
    10681 agttcttcgt gtgtcagcgg acgatcacga cgtatctggt tcgcataacc aaaacgactg
    10741 gctaatctca tgatttgctc cttatgaatg attaagcgat ataaatgaaa aagccgcgtc
    10801 tcccggagaa gacgcggcct gatgggtgaa atgaatgagg tttattgtct gagaagccct
    10861 tcactggcga accacgtatt aagctgtatt ccggcatcgc cagtgcaact gaccttcagc
    10921 attgcggata acccgccgga aatatgttca ctggttgttt ttgtatcttc ccccaaaatt
    10981 aaataaattc atctt
//
LOCUS       DAZFAB010000133        10907 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZFAB010000133 DAZFAB010000000
VERSION     DAZFAB010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10907
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.133"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            63..824
                     /gene="adiY"
                     /locus_tag="ACHJWA_004145"
     CDS             63..824
                     /gene="adiY"
                     /locus_tag="ACHJWA_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFO4310632.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            961..2298
                     /gene="adiC"
                     /locus_tag="ACHJWA_004146"
     CDS             961..2298
                     /gene="adiC"
                     /locus_tag="ACHJWA_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFO4310633.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2402..4045
                     /gene="eptA"
                     /locus_tag="ACHJWA_004147"
     CDS             2402..4045
                     /gene="eptA"
                     /locus_tag="ACHJWA_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFO4310634.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4042..4710
                     /gene="basR"
                     /locus_tag="ACHJWA_004148"
     CDS             4042..4710
                     /gene="basR"
                     /locus_tag="ACHJWA_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFO4310635.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4711..5811
                     /gene="pmrB"
                     /locus_tag="ACHJWA_004149"
     CDS             4711..5811
                     /gene="pmrB"
                     /locus_tag="ACHJWA_004149"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFO4310636.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(5787..5876)
                     /gene="pmrR"
                     /locus_tag="ACHJWA_004150"
     CDS             complement(5787..5876)
                     /gene="pmrR"
                     /locus_tag="ACHJWA_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFO4310637.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(5988..7490)
                     /gene="proP"
                     /locus_tag="ACHJWA_004151"
     CDS             complement(5988..7490)
                     /gene="proP"
                     /locus_tag="ACHJWA_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFO4310638.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVVGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7754..8632)
                     /gene="rdcB"
                     /locus_tag="ACHJWA_004152"
     CDS             complement(7754..8632)
                     /gene="rdcB"
                     /locus_tag="ACHJWA_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HFO4310639.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMREEIFSVNLV"
     gene            complement(8629..10857)
                     /gene="crfC"
                     /locus_tag="ACHJWA_004153"
                     /pseudo
     CDS             complement(8629..10857)
                     /gene="crfC"
                     /locus_tag="ACHJWA_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313118.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
BASE COUNT         2724 a         2861 c         2690 g         2632 t
ORIGIN      
        1 tttcccggta tcgctttgct ttagcgagaa ctggtctttt attcgcactc aggagtacat
       61 gtatgaggat ttgcagcgac caaccttgta ttgttttatt gactgaaaaa gatgtctgga
      121 taagggttaa tgggaaagaa cctattagcc ttaaagctaa ccatatggcg ttattaaatt
      181 gtgaaaataa tattatcgac gtctcctctc ttaacaacac tttggttgct catattagtc
      241 acgacatcat caaagattac ctccggtttc tgaataaaga tctctcgcaa ataccagtat
      301 ggcaacgtag cgctacgccc attctcaccc tgccatgcct gacaccagac gtctttcgcg
      361 ttgccgcgca acacagcatg atgcccgcag aaactgagtc agaaaaggaa cgaacacgcg
      421 cattattatt cacggtgcta tcccgttttc tcgacagtaa aaaattcctt tcattaatga
      481 tgtatatgtt acgtaattgt gtaagtgaca gcgtttatca aattattgaa agcgatattc
      541 acaaagactg gaatcttagt atggtagcca gttgtttatg tcttagccca agtctgttaa
      601 agaaaaagct gaaaagcgaa aacaccagtt atagccaaat aatcaccacc tgccgcatgc
      661 gttatgccgt aaatgaatta atgatggacg gtaaaaatat ctctcaggta tcacagtcct
      721 gcggctacaa cagtacgtcg tactttattt ctgtctttaa agacttctac ggtatgacac
      781 cgctgcatta tgttagtcag cacagagaac gcactgtcgc ctgattttta accttaacga
      841 agagctatat taataacggc atcagcgata acccggtcga taataattca actatcgaat
      901 gcaggcgtat gatatgacgt aatttattgt cacgaagctc gccttcgcag gagtttaatt
      961 atgtcttcgg atgctgatgc tcacaaagtg ggcttaatcc ccgtcaccct gatggtgtcg
     1021 gggaatatta tggggtcagg tgtttttctg ttacctgcaa acctggcctc caccggcggg
     1081 attgccattt atggatggtt ggtgacgatt atcggtgcgc tggggctctc gatggtatac
     1141 gccaaaatgt cgttcctcga cccaagtcct ggtggttctt acgcttacgc ccgccgctgc
     1201 tttggcccgt ttctcggtta tcaaaccaac gtcctctact ggctggcctg ctggatcggc
     1261 aatatcgcca tggtggtcat tggcgtagga tatttaagtt acttcttccc gattctgaaa
     1321 gatccattgg tattaaccat cacctgcgtc gtggtgctgt ggatcttcgt cctgctgaac
     1381 attgtcggtc cgaaaatgat cacccgtgtg caggccgttg ccaccgtgct ggcgctgatc
     1441 cccatcgtcg ggattgccgt atttggctgg ttctggtttc gtggtgaaac ctatatggcg
     1501 gcatggaacg tcagcggcct gggcaccttc ggtgcaattc aaagtaccct taacgttacg
     1561 ctgtggtcgt tcatcggtgt ggaaagtgcc tccgttgccg caggtgtggt gaaaaacccg
     1621 aaacgcaacg tccctatagc caccattggt ggggtattga ttgccgccgt ttgctatgtg
     1681 ctctccacca ccgcgattat ggggatgatc cctaatgccg cactgcgcgt ttctgcctcg
     1741 ccattcggtg atgccgcacg gatggcgttg ggtgacaccg ccggggccat cgtttccttc
     1801 tgcgcagctg cgggttgctt aggttcactg ggcggctgga cattgctggc gggtcaaacg
     1861 gcgaaagcgg ctgccgatga cggactgttc ccaccgattt ttgcccgtgt aaataaagcg
     1921 ggtacgccag tggcggggct gattatcgtc ggtattttga tgaccatctt ccagctcagc
     1981 agcatttcac caaacgcgac caaagaattc ggtctggttt cttccgtgtc ggtcatcttt
     2041 acactggtgc catatcttta cacctgtgcg gcgttactgc tgctcggaca cggtcacttt
     2101 ggtaaagcac gcccggcata tctggcggtt actaccattg ccttcctcta ctgcatctgg
     2161 gccgtggtgg ggtccggggc gaaagaggtt atgtggtcat ttgtcaccct gatggtcatc
     2221 accgccatgt atgcgctgaa ttacaaccgg ctgcataaaa acccgtatcc cttagatgca
     2281 ccaataagca aagattaatt ccccttaatc cagcaaagat aaaagccaac cttaagaact
     2341 taaggttggc ttaattttgc tttgcgagca tatgcgcact ttgttcgatg gaaacaccgt
     2401 gatgttgaag cgcctactaa aaagaccctc tttgaattta ctcgcctggc tattgttggc
     2461 cgctttttat atctctatct gcctgaatat tgcctttttt aaacaggtgt tgcaggcgct
     2521 gccgctggac tcgctgcata acgtactggt tttcttgtcg atgccggtcg tagccttcag
     2581 cgtgattaat attgtcctga cactaagctc tttcttatgg cttaatcgac cgctggcctg
     2641 cctgtttatt ctggttggcg cggctgcaca atatttcata atgacttacg gcatcgtcat
     2701 cgaccgctcg atgattgcca atattattga taccactccg gcagaaagct atgcgctgat
     2761 gacaccgcaa atgttattaa cgctgggatt cagcggcgtg cttgctgcgc tgattgcctg
     2821 ctggattaaa atcaaacctg ccacctcgcg tctgcgcagt gttcttttcc gtggagccaa
     2881 tattctggtt tctgtactgc tgattttgct ggtcgccgca ctattttata aagactacgc
     2941 ctcgttgttc cgcaacaaca aagagctggt gaagtcctta agcccctcta acagcattgt
     3001 tgccagctgg tcatggtact cccatcagcg actggcaaat ctgccgctgg tgcgaattgg
     3061 tgaagacgcg caccgcaacc cattaatgca gaacgaaaaa cgtaaaaatt tgaccatcct
     3121 gattgtcggc gaaacctcgc gggcggagaa cttctccctc aacggctacc cgcgtgaaac
     3181 taacccgcgg ttggcgaaag ataacgtggt ctatttccct aataccgcat cttgcggcac
     3241 ggcaacggca gtttcagtac cgtgcatgtt ctcggatatg ccgcgagagc actacaaaga
     3301 agagctggca cagcaccagg aaggcgtgct ggatatcatt cagcgagcgg gcatcaacgt
     3361 gctgtggaat gacaacgatg gcggctgtaa aggtgcctgc gaccgcgtgc ctcaccagaa
     3421 cgtcaccgcg ctgaatctgc ctgatcagtg catcaacggc gaatgctatg acgaagtgct
     3481 gttccacggg ctggaagagt acatcaataa cctgcagggt gatggcgtga ttgtcttaca
     3541 caccatcggc agccacggtc cgacctatta caaccgctat ccgccgcagt tcaggaaatt
     3601 taccccaacc tgcgacacca atgagatcca gacctgtacc aaagagcaac tggtgaacac
     3661 ttacgacaac acgctggttt acgtcgacta tattgttgat aaagcgatta atctgctgaa
     3721 agaacatcag gataaattta ccaccagcct ggtttatctt tctgaccacg gtgaatcgtt
     3781 aggtgaaaat ggcatctatc tgcacggtct gccttatgcc atcgccccgg atagccaaaa
     3841 acaggtgccg atgctgctgt ggctgtcgga ggattatcaa aaacggtatc aggttgacca
     3901 gaactgcctg caaaaacagg cgcaaacgca acactattca caagacaatt tattctccac
     3961 gctattggga ttaactggcg ttgagacgaa gtattaccag gctgcggatg atattctgca
     4021 aacttgcagg agagtgagtg aatgaaaatt ctgattgttg aagacgatac gctgttattg
     4081 cagggactga ttctggcggc gcaaaccgaa ggctacgcgt gcgatggcgt gacaaccgcg
     4141 cggatggcgg aacaaagcct tgaggcaggt cattacagcc tggtggtact ggatttaggg
     4201 ttacccgacg aagatggact gcattttctc gcccgtatcc ggcagaaaaa atataccctg
     4261 ccggtactga tcctcaccgc tcgcgatacg ctgaccgaca aaatcgccgg gctggatgtc
     4321 ggtgccgacg actatctggt gaagcctttt gcgctggaag agttacatgc ccgtatccgc
     4381 gccctgctac gacgccataa taatcagggc gaaagtgagc tgattgttgg caatctgacg
     4441 ctgaacatgg gtcgccgtca ggtatggatg ggcggtgaag agttgattct gacgcccaaa
     4501 gaatatgctc tgctgtcacg gttaatgctc aaagcaggca gtccggtgca tcgggaaatt
     4561 ctctacaacg acatctataa ctgggacaat gaaccctcga ccaacaccct ggaagtgcat
     4621 atccacaatc tgcgcgacaa agtgggcaaa gcccgtatcc gcaccgtgcg cggctttggc
     4681 tatatgcttg tcgcgaatga ggaaaactaa ttgaatctga tgcgttttct gcgccgacca
     4741 atatcgctgc gccaacggct gatattgacc atcggggcca ttttgttggt gtttgagctg
     4801 atcagcgtct tctggctatg gcatgaaagt accgagcaga ttcagctgtt tgagcaggcg
     4861 ctacgcgaca atcgcaacaa cgatcgccac atcatgcgtg agatccgcga ggcggtcgcc
     4921 agcctgattg tccccggcgt ctttatggtc agcctgacgc tatttatctg ctatcaggcg
     4981 gtacgccgca tcacccgccc gctggcggag ctgcaaaaag agctggaagc gcgcaccgcc
     5041 gacaacttaa cgcccattgc cattcacagc gccaccctcg aaatcgaagc ggtggtttcg
     5101 gcgttgaacg atctggtcag tcgcctgacc agcacgctgg ataacgaaag gttatttacc
     5161 gctgacgtcg cgcacgaact gcgaacgcca ctggcggggg tgcgtttgca tctggaactg
     5221 ctggcgaaaa cgcatcacat tgatgtagca ccgttagtgg cacggcttga tcagatgatg
     5281 gagagcgtct cccagctgct gcaactggcg cgtgccggac agtcattttc ttccggtaat
     5341 tatcaacatg taaaactgct ggaagatgtc attctcccct cgtatgacga actcagtacc
     5401 atgctcgacc agcgacagca aaccctgcta ttgccagaga gcgccgccga catcaccgtt
     5461 cagggtgatg caaccctgct gcggatgtta ttgcgaaacc tggtagaaaa cgcccatcgt
     5521 tacagcccgc aaggcagcaa cattatgatt aagctgcaag aagatggcgg agcggtcatg
     5581 gccgttgaag atgaaggacc gggtattgat gagagtaaat gcggggagtt gagtaaagcg
     5641 tttgtacgta tggacagccg ttatggcggg attggtctgg ggttaagtat tgtcagccgc
     5701 attacacagt tgcatcacgg gcagtttttc ctgcaaaacc ggcaagagac ttccggcacg
     5761 cgggcctggg tacggctgaa gaaagatcag aacgtggcaa accagatata aagaaagctg
     5821 ctgaccacca gcacgctgaa cacggtagtt aaactttcat aaacacggtt tttcattgca
     5881 ctctcctccg ttaacctgga ggagagtatg cgcgtcagag attaagtgaa ccttaagagt
     5941 tcaattggcc tgataagaca gcgttacatc aggccatccg tttcagctta ttcatcaatt
     6001 cgcggatgtt gctgcaccag gcgggtacgt ttcgcctgca aatcggcaat ctcgtggtca
     6061 atatcatcga ttttctgctc gatattatcg taatgctcga cgagaatttc cttcgcttcc
     6121 tgtatatctg acgccgccgg tgttgcacct ttcaacggac gatttgccgt ctctttcatg
     6181 gttacgccgg tgattaaacc aaccaccgcc actaccatca ggtaataggc tggcatcatc
     6241 agattctgcg agctttcgac cagccaggcc gccagtgttg gcgtcagacc ggcaaccagc
     6301 accgaaatat taaatgccgc cgccagcgcg ctgtaacgga tatgcgtcgg gaacatcgct
     6361 ggcaaggtag aagccataac gcccgtaaag cagttaagga tcaccgccag catcagtaac
     6421 ccggcaaaaa tcaggccgat gacgttactg ttaatcagaa taaacgccgg gatcgccaac
     6481 acaaacagtg caacactacc aagtagcaca aacggacgac ggccaaaacg gtcactcagc
     6541 aagcccatca ccggctggac aaacagcata ccgatcataa tggcgataat aatcagcacc
     6601 ccgtggtctt cggagtaatg caggttatgc gacaaataac tcggcatata ggtcagcaac
     6661 atgtagtaag tcacgttggt ggcaattacc agaccaatac atgtcaacag gctgcgccag
     6721 tatttagtgg caatctcttt aaacgagact ttcgggccat cctgcaaacc ttcgcggtcg
     6781 ccctgttcca gtttatcaac atgctgctgg aacgccggag tttcttccag cgcatggcgc
     6841 aggtaaagcc cgataatccc taacggcaga gcaataaaga acggaatacg ccagccccag
     6901 tcgaggaagt tcgcttcgcc gacaatggtc gaaattaaca ccaccacgcc cgcacccagc
     6961 acaaacccgg caatagaacc gaagtccagc cagctgccca taaagccacg tttacggtca
     7021 ggggagtatt ccgcaacaaa tatcgacgcc ccggtatatt caccgccgac cgagaaacct
     7081 tgtgccatct tacagatcaa cagcagaatc ggtgcccaaa taccaatcgt gtcgtaggac
     7141 ggtattaagc caatacagaa cgtactgatc gacataatca caatagtgat agcgaggatc
     7201 ttctggcgac catatttatc gcccaacata ccaaagaaga gtccgccaag cggtcgaatc
     7261 agaaagggaa cggagaaagt ggcaagtgca gcaaccatct gcacgctggg gtcagccccc
     7321 gggaaaaaaa ctttacctaa tgcgtaagca acaaaaccat aaacaccaaa atcaaaccat
     7381 tccattgcat tacccagtga tgctgcggta atggctttac gcagtttacc gtcatcaata
     7441 atggtgacat cacgaagggt aatcggtttt acttttttcc ttttcagcat agctttcctc
     7501 gcagagttgg cccatttccg caaacgccag aaaaatcagc gcgacggccg caaaagggga
     7561 gcgggaattg caggatgcaa tctctgtaac aagcgtagcg ggtttgctta caccctccgg
     7621 ttaaggaatg tacaatcaaa ccagttgacg cctgaattgg gcagttaatg acctttttac
     7681 cctaccagcg tttaaatttg tgatcaactt cacacatgga tttcgtttag gactcattga
     7741 tgtaactgat tatttacacc aaatttacag agaagatttc ctcgcgcatt gcccccgcca
     7801 gacgctgcgc tgaacagagt cgtggcatcc ccagcgcgac agtttgccag cgttgggtca
     7861 ctgaccagca aacgggttgt ttatctgccg tggtccactt tcccagccaa cttaaaaaat
     7921 cagcatgatc cccgcccgtc ggaacggtcg aagaggcaaa ccactgatgg taatagtgac
     7981 gcgtggtcgg ggccgcattg atactctctg ccagatgttg tgtcgctttc acgcgcaaat
     8041 cacccagcaa caacgccacc gcttgcgggc ttcccagacg acaggcatcg ccaaatgccc
     8101 cccaacgcaa ctcttcgaga acgataaaac agcgcccagg caacgaccag gcgttatacg
     8161 ccccagcacg ccagcgggta aaaatttgct cgctctgttc acccgcctgc accagtaggc
     8221 ctcgttgcgt taacgccttt tccttccagc gacaacgggc tgaaaaccac tcatacaagg
     8281 cggcaatttg ctgggggagc tccgggaaag cagagtgctg gcggtgatcc attttttgca
     8341 gcagcgccag atgctgcgcg atctgcgtca gtgccagatg accgggatcc agcatcgacg
     8401 ccagtttttc caccaatgtc agacgcagac taagcggcgc atttaacatc ccgccgttcg
     8461 tccatgtctg aagctgcacg cgcgcaagaa gttcctgcat cagtcgttca cgaaactgct
     8521 gctgatgggc ttgtggaagg cgggcatcat cgccctgcgc cagatccact aaaaagcggg
     8581 gataaaccga ctccagcacg cgaccggggc cgtccagtaa cgtcttggtc aatatcgttc
     8641 tgccgtgaaa agtgtttgaa tatcatcgcg taacagctga gcgtcggtgt aaatccagcc
     8701 gtgagtcatc acagtctgct gcaattgctg ttgcatcagc ctgaccaccg attcattttg
     8761 ttgacgcaga gccagacttt cgcgtaaacg cgtctgtaat tccgtcaaac atgaagcgaa
     8821 ctcagcgaaa aaagtattca tgcctgccgt aacagattca tcgacctgct ctgccagaac
     8881 tttagccatt tgttggcaat aaagatcgac ttctgcgctt aatgctcgtt gcaacacact
     8941 gtaatcaacc gtttctgtcg gggatttctc atttccccgt ccccagtcag gctgattcaa
     9001 ccagcgcgaa aaagtctcac gcacaacgcc taatcgcgtg ctctgctcgt ccgttgcttc
     9061 ctggcgcgaa atgacttcac tgaacagctg gcgagtgttg aagtggggaa ctacgccgtg
     9121 aaaaacagga aaatgaaacc caggacgaaa ccctgactcg ctcaattcca ttttgacttg
     9181 ttgctcaatg gggcgaataa catcggttaa cacccggcaa agggtggatt caagctcggc
     9241 aaaacgcagc gtaaagtcgc gactgatggt gttctgcgcc gtctgtaaca gtgtctcaca
     9301 gcgggtacga atctcgctta acggctccga atcatcctga aacaaggcgg ctaactgcac
     9361 cttcagcgca tcttgctgtt gacgcagaaa gtggttggcg gaggtcaggg ccagctcgat
     9421 ttcatgttta atctcgccgc tcacctgcgc ctgattgagt tgcaatagct gcaaactttc
     9481 ttcgatctgg tggatatttt gccgcaattg ttcacaagcg acgtttaacc cgtgcgcacg
     9541 aaaatccagg tattcccgcg cctgctgcgc gtaattcaac agtttatgcg cggcagatcg
     9601 cagagcatac aacgaggcgt tagcgtaagc ggcatgaagc aacatctgaa ttggctgggc
     9661 gaacagcgaa tcttcccaca actgatcggc agcatgacga atatgttcga ggtccgccag
     9721 atcggcatga cgccagcgcc tgccgagcgc ggcatgggca aaatcttcca cccagcgttg
     9781 ttgctctggc ggtggtaact taccgttgtt ggctaactca tggcgcgccc gatttgccag
     9841 gtagccccac atcgacgaca ccggaaatat ctgctgtggc gtaatacagc ctttcatcag
     9901 cgtcccggaa atcagtgccc gcacctggtc ggcgtcgtca ctgttacgat cctgttgatc
     9961 gaacttattg accagcacat acagcggcac cgattgcccc accgccaaaa tcgcctcacg
    10021 gacctcttca tcggagatcg atttcagttg cgtataatcc agcaccgcca gtaccgccga
    10081 ggcgcgtgcc agctgctggt taagcatttt ttgcagatgc ggttgcccgg cttcatttgg
    10141 cccgggggta tccagtaacg tcaattgacc gggataactt tccagccccg ccagatggac
    10201 aaactccact tcaatcacgg gaatatgctc aatggcggcg taagcagaaa aaggaaaatc
    10261 gacgtccagc gccttcgcca gtcgcaccaa atcattcaaa cttttcagac aatgaaaaat
    10321 aggctgggca cccagataat atttttcgaa agcgacgcca ttttcgatcc gctgcataag
    10381 cgcacgcata tctttatcta tttccagcac atcggtcaga tgcttaatat cgcaatcacg
    10441 caggcgctgt tgtaattttt aaattaaaca atcgattggc gcgacatgtg aaaaatgcag
    10501 taccggttcc ttttggccgg gcgtatggcg aataagcgtc ggcagcgcag tcattgggcg
    10561 attgcgatta ggcagtacct ccgtaccaac aatggcatta atggtggttg atttccctgc
    10621 tttcatggta ccgacaatcg ccagcaccat ttccagtcgg gaaattttac gcaactcatt
    10681 attcagcatc gcgtgacgct cggcgatatt aggctgactc cagggtaaag ccagttgtgg
    10741 cgcatcgtct ccgggtacag agagaggcat tttttccagt aactgcaact gttggcgaga
    10801 aagctgtaac agccgttcag cctcctgact taactcatac agggtctgtg tgtacataga
    10861 aaattcttcc ttaaagcaaa ttttattact tttatttagc cagattg
//
LOCUS       DAZFAB010000134        10858 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZFAB010000134 DAZFAB010000000
VERSION     DAZFAB010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10858
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.134"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(310..1902)
                     /gene="lsrK"
                     /locus_tag="ACHJWA_004154"
     CDS             complement(310..1902)
                     /gene="lsrK"
                     /locus_tag="ACHJWA_004154"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310145.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071518 - autoinducer-2 kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HFO4310640.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTIRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKVGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSTIHPSSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(1981..2934)
                     /gene="lsrR"
                     /locus_tag="ACHJWA_004155"
     CDS             complement(1981..2934)
                     /gene="lsrR"
                     /locus_tag="ACHJWA_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HFO4310641.1"
                     /translation="MTINDSVISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            3183..4718
                     /gene="lsrA"
                     /locus_tag="ACHJWA_004156"
     CDS             3183..4718
                     /gene="lsrA"
                     /locus_tag="ACHJWA_004156"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HFO4310642.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITSADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFTRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAAGTSVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNDKEINRLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLELYRRALNI
                     KYNQPEQAARTLSGGNQQKILIAKCLEASPQVLIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     AAC"
     gene            4712..5752
                     /gene="lsrC"
                     /locus_tag="ACHJWA_004157"
     CDS             4712..5752
                     /gene="lsrC"
                     /locus_tag="ACHJWA_004157"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HFO4310643.1"
                     /translation="MLKFIQNNREITALLAVLLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAVLGAWFL
                     TQIDSVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALELNLRRQKYARFMTPPPSV
                     KPASSGKKREAA"
     gene            5752..6744
                     /gene="lsrD"
                     /locus_tag="ACHJWA_004158"
     CDS             5752..6744
                     /gene="lsrD"
                     /locus_tag="ACHJWA_004158"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HFO4310644.1"
                     /translation="MRIRYGWELALAALLVIEIVSFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGYPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGAIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6756..7778
                     /gene="lsrB"
                     /locus_tag="ACHJWA_004159"
     CDS             6756..7778
                     /gene="lsrB"
                     /locus_tag="ACHJWA_004159"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HFO4310645.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELGVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            7805..8680
                     /gene="lsrF"
                     /locus_tag="ACHJWA_004160"
     CDS             7805..8680
                     /gene="lsrF"
                     /locus_tag="ACHJWA_004160"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HFO4310646.1"
                     /translation="MADLDDIKDGKDFRTDQPQKNIPFTLKGCGALDWGMQSRLSRIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGTQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            8704..8994
                     /gene="lsrG"
                     /locus_tag="ACHJWA_004161"
     CDS             8704..8994
                     /gene="lsrG"
                     /locus_tag="ACHJWA_004161"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HFO4310647.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            9051..9809
                     /gene="tam"
                     /locus_tag="ACHJWA_004162"
     CDS             9051..9809
                     /gene="tam"
                     /locus_tag="ACHJWA_004162"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFO4310648.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENVDYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGRESLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLKEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(9813..10727)
                     /locus_tag="ACHJWA_004163"
     CDS             complement(9813..10727)
                     /locus_tag="ACHJWA_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFO4310649.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQVEVLAHYLKTEDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKVLPDRHYQLT"
BASE COUNT         2674 a         2668 c         2804 g         2712 t
ORIGIN      
        1 aaaactgttt ctttcagtag gaaaattatc atcatcatat ttaacaaaga atagcaccaa
       61 ttgctaaaaa tcgaagttta ttaaacccct tttgcatcca ttaatgtctt taggaatatt
      121 cgttataaaa taagggttat gcttaatgct ttaagaaaaa tagcaatttc cccttgaata
      181 tagtactggt gatgaaacga tgaatctgca agctggcatt taacaagcca gctctaaaag
      241 aagggaaata agaataacta tactcaaaaa ctaacagcca cggtcatcat gatgtggctg
      301 tcaatgaaac tataacccag gcgctttcca taacgacgtc gtcagtccat gatcaaccag
      361 ccccagctga tcctgataaa ctgcctgcca cttatcgcgt gaatcctgat aaagttcatg
      421 cttttccggg tctggtgtgt gcgtccgttc ccagcgaacc aggcgttctc cggtttctgc
      481 cattgatgaa aaaattccgg caccgacgcc agctgcaatg gcacatccta atgcagtggc
      541 ttctttgacc accggaatat tgacgggtaa tcccgagaca tcagcgagaa tttgactcca
      601 taatttccct tttgaacctc cgcctgcaaa gactaacgat gaaggatgaa tagtcgaaaa
      661 atcagcaatt tgctgcaagt tacacgctga tacaatcgcc gcattttctt ccagcgcacg
      721 gaacaatgtc gctttgttac atttatccgg atcaatggac aaattaataa aggaaggcgc
      781 agcgtgatac caggttttaa agcgcattct gtcggagaag atcggcatta cgccccacga
      841 cccaggcggc acccgactgg ccatctcttc cagcagcgta taggtgtcga tgcctaaacg
      901 ttcggcaatc agtttttctt cggcacagaa agcatcgcgg aaccagcgca tggtgagtcc
      961 ggtaaaaaag cttatagatt cagcttgtac catgccagga ataacatgag gattaacgcg
     1021 cacgttcatt tctgggtctg tcaccggcgc ggctaaattt acaacttgct gccagaatgt
     1081 gccgccaaga accgcggttt gtgccggacg cacaacgcct aaccccaggc aaccaagctg
     1141 cacgtcgcct cctccaacga ccaccggagt gcccaccttc agaccgcaga gttccgccgc
     1201 ttgtgaactt accacgccca gcaatgtgcc ggtttctttg acaggagaaa gaatatcggc
     1261 acgcaggcca gccatatcca gcaatgcagg tttccagtca cggatggtta gatcaagaag
     1321 tcccgtggtg ccagcgttag agggatccac cgcaagttcg ccgctgagca tataggccag
     1381 ccagtcgctg atcatggtga tggttgatgc ctgacggtaa atatcggaac gatggtgcgc
     1441 cagccaaagt aatctgggga tggcacttaa agccagtgtt tgtccggtcg cgcgataaac
     1501 ttcgttttca aaggtattgt tgtgcagttc tttaagttcg ctaacttcgc gcgccgctct
     1561 ggcatccaca ttggcgcagg cccagatcgg tgttccttca ttgttatata aaacaatgcc
     1621 ttcacgcatc gaacatgccg aaacggcagc gatatactcc ggggctatgc cggcgttgtg
     1681 cagcgcctgg cgcatacact cacacgccag ttgccagttt ttgttgagat caaattccat
     1741 agaaccagga acgtccggta ctgccagatg ccgccactcc gcctgtccca ctgctatttg
     1801 attgccttcc aggtcgaata tcacagcccg aatacttccg gtgcctgcat ccagcgccat
     1861 caggtagtac tttgattctg aaggggtaaa gagtcgagcc attagattat cctcggctta
     1921 tgcgttctgt ctgttcctct atacgttctc catcattccc ggtaataagg tcatgcaaat
     1981 ttaactacgt aaaatcgccg ctgctgtgtc ctgatcggta accagtgcgt tgatataacc
     2041 gcctttcatt gcagcggcaa ttgcttcggc tttattttct ccccctgcca cgccaacccg
     2101 gacgggtatg gtcttcagcg cgcttaaagg taagccaatc agctcgttat gtattttaat
     2161 gtccgtgaca acgtcacctt ttgcatcaaa aaagtagcct aaaatgtcgc caaccgcccc
     2221 ttttcggcca atcattaact gttcgccctg gctgatataa ccggagcgaa tgatcgtcgc
     2281 gtcgtcttgt tgactaacgg ccccaatgcc gacaatcgcc acatccgctg cttgcgcggc
     2341 taacagaaca tctttgacac aattttcatt ttttagcgta cgggcaatgt cagcggagga
     2401 tgcccgcaac ggagccggaa taatattcac actgcacgcc gcattaagct gtccgatacc
     2461 cgtcatataa gaaccgacgc caccggagag cgtgaccagg cgaatttgct gtgacgaaat
     2521 aaaaccactt aagcgttgca gcgtattcat ggttgcctcg ccaaaaccaa tcgccagcat
     2581 ctgttgtggt tgaagtaaac tcatcaacat atgcgccgcg cctatcccca gtcgcccgcc
     2641 gacatcagca tccgcaagcc cagggatcac ccggacatgt tgcagcgaaa actgacgacg
     2701 taattgtgtt tcatattcca gacagccttc aaagcgagaa ttaatctgca cgcgaatgat
     2761 gccggactga tgccctttct ccagtaatcg cgacactttc aaacgggtca ggccgagacg
     2821 atcgctgatc tcactctggg tcagcccgtc gtggtagtaa aaccacgcga tccgcgcgac
     2881 ctgttcttct tcacacattc cctgttctga aattaccgaa tcgttgattg tcataattca
     2941 ttcttcactt tgaacatatt taaatcttta atgcaattgt tcagttcttg cgcatttata
     3001 tctgtgatgg caaccacagt ttgactctac gagcatgaac aaacgcaacc gtgaaaatca
     3061 aaatagtata aattgtgatc tattcgtcgg aaatatgtgc aatgtccacc taaggttatg
     3121 aacaaattaa aagcagaaat acatttgttc aaaactcacc tgcaaaactg aacgggggaa
     3181 atatgcaaac gagtgatacc cgcgcgttac cgctactttg cgcccgctcg gtttataaac
     3241 agtattcagg ggtcaatgtc ctgaaaggca tcgattttac gttgcatcag ggggaggtcc
     3301 acgccctgct cggcggcaat ggtgccggta aatcgacgtt aatgaagatt attgccggta
     3361 ttacctctgc tgatagcggt acgctggaga ttgggggcaa caactacgcc agattaacgc
     3421 cagttcatgc tcatcagctg ggtatttatc tcgttcccca ggaaccgctg cttttcccaa
     3481 gcctgtcgat aaaagaaaac atcctgtttg ggctggcaaa aaaacagctc tccatgcaga
     3541 aaatgaagaa cttgctggcg gcgctgggct gccagtttga tctgcatagt ctggcaggat
     3601 cgctggatgt cgccgatcgc caaatggtgg aaatcctccg cgggctgatg cgcgactcgc
     3661 ggattctgat cctcgatgaa cctaccgcct cgcttacccc tgcggaaacc gaacgcttgt
     3721 ttactcgctt gcaagagctg cttgctactg gcgtgggtat tgtttttatc tcgcataagc
     3781 tgccggaaat tcgccagatt gccgatcgga ttagcgtgat gcgcgacgga accatcgcct
     3841 taagcggcaa aaccagcgaa ctgtctaccg acgacattat tcaggccatc accccagcgg
     3901 tacgggaaaa atcgctctct gccagccaaa aattatggct ggagttacct ggcaaccgcc
     3961 cacaacatgc cgcaggaacg tcggtgctga cactggaaaa tctgactggc gaaggtttca
     4021 ggaatgtcag cctgacgcta aatgccggag aaattctggg tctggctggg ctggtgggtg
     4081 ccggacgcac agaactggcc gagacgctct atggtctacg tactttgcgt ggcggacgca
     4141 ttatgctgaa tgataaagag atcaatagat tatccactgg agaacgttta ctgcgcggtc
     4201 tggtttatct gccggaagat cgccagtcat ccggactgaa tctcgatgct tcgctggcct
     4261 ggaacgtctg cgcccttact cataaccttc gtggattctg ggcgaaaacc gcgaaagata
     4321 atgccaccct ggaactttat cgtcgggcgc tgaatattaa atataaccaa ccggaacaag
     4381 ctgcacggac attatccggt ggtaaccagc aaaaaatcct gattgccaaa tgcctggaag
     4441 cctcgccgca agtattgatt gtcgatgagc cgacgcgcgg cgtggatgtc tcggcgcgta
     4501 atgatatcta ccaactgttg cgcagcatcg ccgcacagaa tgtggctgtg ctgcttatct
     4561 cctctgatct ggaagagatc gaactgatgg cagatcgtgt gtatgtgatg catcagggcg
     4621 aaattaccca ctctgcactg accgggcgcg atattaatgt cgagactatt atgcgcgttg
     4681 ccttcggcga tagtcagcgt caggaggcgg catgctgaag tttattcaga acaaccgtga
     4741 aatcacggca ctgctggcgg tgctgctgct gtttgtatta cccggttttc tcgaccgcca
     4801 gtatttaagt gtgcaaacgc tgaccatggt ttatagcagc gcgcaaatcc tgatcctgct
     4861 ggcaatgggc gcgacgctgg taatgcttac gcgcaatatt gatgtttcag tgggttcgat
     4921 taccggaatg tgcgcggtgc tgttggggat gttactgaac gcaggatatt cactacctgt
     4981 tgcctgtgtc gcgactttac tgcttggttt gctcgcggga tttttcaacg gtgtcctggt
     5041 cgcgtggcta aagatccctg ccattgttgc cacccttggc acgttagggt tgtacagagg
     5101 catcatgttg ctgtggactg gcggtaaatg gattgaaggg ttacccgccg aactgaaaca
     5161 gctctccgcc ccgctgctgc ttggcgtttc agcaattggt tggttaacga taattctggt
     5221 ggcatttatg gcctggctgc tggcgaagac agcgtttgga cgcagttttt atgccacggg
     5281 cgataattta cagggcgctc gtcaactggg cgttcgtacg gaagccattc gcattgtggc
     5341 attttcgttg aacggctgca tggcggcact ggcgggaatt gtgtttgctt cgcagattgg
     5401 ttttatcccc aaccagaccg gtaccgggct ggagatgaaa gcaattgcag cctgcgtgct
     5461 gggcggcatt agtttgctcg gcggttccgg tgcgatcatc ggtgcggtac tcggtgcggt
     5521 actcggcgca tggttcctga cgcagatcga tagcgtactg gtgctgttgc gcattccggc
     5581 atggtggaat gattttatcg cgggtctggt tctgctggcg gtgctggtgt ttgatggacg
     5641 cctgcgttgt gcgctggaac ttaatctgcg gcggcaaaaa tatgcccgct ttatgacgcc
     5701 accgccatcc gttaaacccg cttcgtcagg taaaaaacgg gaggctgcat aatgcgtatt
     5761 cgctacggtt gggaactggc tcttgccgca ctgcttgtta ttgagattgt ctcatttggt
     5821 gcaattaacc cgcggatgtt agatctcaat atgttgctgt tcagcaccag tgactttatc
     5881 tgcattggca ttgtcgccct accgctgacg atggtgattg tcagtggcgg gatcgatatt
     5941 tcgtttggtt cgaccatcgg cctctgcgcc attgcattgg gcgtactgtt tcaaagtggt
     6001 gtgccgatgc cgctggcgat tctcctgacc ttactgctcg gcgcattgtg cgggctgatc
     6061 aacgccggat taattatcta taccaaagtt aacccgctgg tgattacgct tggcacgctg
     6121 tatctgtttg ccggaagcgc cctgctgctt tccgggatgg ccggggcgac ggggtacgaa
     6181 ggtattggtg gatacccgat ggcgtttaca gatttcgcta acctggatgt gttggggctc
     6241 cccgttccgc tgattatctt cctgatatgt ctcctcgttt tctggctctg gctgcataaa
     6301 acccatgccg gacgtaacgt gtttttgatt gggcaaagcc cgcgcgtggc gctttatagc
     6361 gcgattccag ttaaccgtac cttatgtgcg ctctatgcca tgacggggct ggcgtctgcg
     6421 gtcgccgctg tgctgctggt gtcgtatttt ggttcagcac gttccgatct cggtgcgtcg
     6481 tttctgatgc ccgccatcac cgccgtggtg cttggcggtg ccaatattta tggtggttcc
     6541 ggtgccatta tcggcaccgc cattgcggtt ttattagtgg gatatttgca acaaggtttg
     6601 caaatggcag gagtgccaaa tcaggtgtcc agcgcccttt ccggtgcgct acttatcgtc
     6661 gttgtcgtag gtcgttccgt tagcctgcat cgccagcaaa ttaaagagtg gctggcgcgt
     6721 cgggccaata acccattgcc ataaaggata tcttcatgac acttcatcgc tttaagaaaa
     6781 tcgccttact tagcgctctt ggcattgccg caatctctat gaatgtgcag gccgcagagc
     6841 gtattgcatt tattcccaaa ctggttggag tgggattttt taccagcggt ggcaacggcg
     6901 cacaacaagc gggtaaagag ctgggcgttg atgtgaccta cgacgggccg acagaaccca
     6961 gtgtttctgg tcaggtacag ttgattaata acttcgtcaa tcaaggttat aacgccatta
     7021 tcgtttctgc ggtttcgcct gatggcttgt gtccagcact gaaacgcgcc atgcaacgtg
     7081 gtgtgagagt gctgacctgg gactctgata ctaaaccgga gtgccgctct tactacatta
     7141 atcagggaac tcccgcccag ttgggaggta tgttggtgga tatggcggcg cgtcaggtga
     7201 ataaagacaa agccaaagtc gcgtttttct actcaagccc caccgttacg gaccaaaacc
     7261 agtgggtgaa agaagcgaaa gcgaaaatcg ccaaagagca tcctggctgg gaaattgtca
     7321 ctacgcagtt tggctataac gatgccacta aatcattaca aaccgcagaa ggaatattaa
     7381 aagcgtatag cgatctcgac gccattatcg cccccgatgc caatgccctg cccgctgccg
     7441 cacaagccgc agaaaacctg aaaaatgaca aagttgcgat tgtcggattc agtacgccaa
     7501 acgtgatgcg tccatatgtg gaacgcggca cggtgaaaga atttggcctg tgggatgtgg
     7561 ttcagcaagg caaaatttca gtttatgtcg cggatgcatt attgaaaaaa ggatcaatga
     7621 aaacgggcga caagctggat attcagggcg taggtcaggt ggaagtctcg ccaaacagcg
     7681 tccagggcta tgactacgaa gcggatggta atggcatcgt actgttaccg gagcgcgtga
     7741 tattcaacaa agagaatatc ggcaaatacg atttctgatg tgcattactt aaccggagta
     7801 agttatggca gatttagacg atattaaaga tggtaaagat tttcgtaccg atcaaccgca
     7861 aaaaaatatc ccttttaccc tgaaaggttg cggtgcgctg gattggggaa tgcagtcacg
     7921 cttatcgcgg atatttaatc cgaaaacggg taaaaccgtg atgctggctt ttgaccatgg
     7981 ttattttcag ggaccaacta ccggacttga acgcattgat ataaatatcg ccccgctgtt
     8041 tgaacatgcc gatgtattaa tgtgtacgcg cgggattttg cgcagcgtag ttcccccggc
     8101 gaccaataag ccggtggtac tgcgggcgtc aggtgcgaac tctatcctgg cggaattaag
     8161 caatgaagcc gtggcgttat cgatggatga cgccgtgcgc ctgaacagtt gcgcggtggc
     8221 ggcgcaggtt tatatcggca gcgaatatga acatcagtcg atcaaaaata ttattcagct
     8281 ggtcgatgcc ggaatgaaag tgggaatgcc gaccatggcc gtgactggcg tgggcaaaga
     8341 catggtgcgc gatcagcgtt atttctcgct cgcgactcga atcgccgctg aaatggggac
     8401 gcaaattatc aaaacctatt atgtcgaaaa aggttttgaa cggattgttg ccggatgtcc
     8461 ggtacccatt gttattgctg gcggtaaaaa attaccggag cgcgaggcgc tggaaatgtg
     8521 ctggcaggct atcgatcagg gcgcttctgg tgtggatatg gggcgtaata ttttccagtc
     8581 tgaccatccg gtggcgatga tgaaagccgt acaggcggtg gttcaccata acgaaacggc
     8641 tgatcgggca tatgaactct atctgagtga aaaacagtaa ctgcggattt aaggagaaga
     8701 attatgcacg tcacactggt tgaaattaac gttcatgaag acaaggttga cgagtttatc
     8761 gaagtttttc gccagaacca cctgggctct gtacaggaag aaggcaattt gcgcttcgat
     8821 gtcttacagg acccggaagt gaattcgcgc ttttatatct acgaagccta taaagatgaa
     8881 gacgcagtgg cgttccataa aaccacgccc cactacaaaa cctgtgtcgc gaaactggaa
     8941 tctttaatga ccgggccgcg taaaaaacgt ctgttcaatg ggttgatgcc gtgaggcgaa
     9001 tttatcagtt ttatctacaa ttggggtaac gcgctgacgg gagtaaataa atgtctgact
     9061 ggaacccctc tttatatctg cactttgccg ctgaacgatc gcgtccggcg gtggagctgc
     9121 ttgccagagt gccgctggag aatgtcgatt atgttgccga tcttggctgt ggcccaggta
     9181 acagcaccgc cctgctgcat caacgttggc ctgctgccag gataacgggc atcgactcgt
     9241 ctccggcgat gattgctgaa gcgcgcagtg ctttgccaga ctgccagttc gtggaagcag
     9301 acatccgcaa ctggcaaccg gaacaggcac tcgatctgat ttttgctaat gcctcactgc
     9361 aatggctgcc cgaccactac gaattgtttc ctcacttggt ttctttactt aatccgcagg
     9421 gcgtgctggc agtacagatg ccagataact ggctggagcc gacccatgtg ctcatgcgcg
     9481 aagttgcctg ggaacaaaac tacccggatc gcgggcgtga gtcgttggct ggtgttcatg
     9541 cttactacga tattttgagc gaagccggat gtgaggtcga tatctggcga accacctact
     9601 atcaccagat gccgtcgcac caggcgatta tcgattgggt gaccgccact ggattacgtc
     9661 cgtggttaca ggatctgacc gagagcgaac agcagctttt tcttacgcgc taccatcaga
     9721 tgctgaaaga gcagtatcca ctgcaagaga acggacagat actgctggca tttccgcgtc
     9781 tgtttattgt tgcccgccgt acggagtaaa tatcacgtca gttggtaatg gcgatcggga
     9841 agaacttttg ctggaatttc ggcttcatcg ttcatctgta agaggtcaat ttcaatagcg
     9901 ttacagatgg catccagcgg taaatcattg ttttcagtac cgaacggatc ttccagttct
     9961 tccgccagac aatccagcga aataaaagta taggaaatca gcacagagat aaaaggcgtc
    10021 atgtaatgca ggtccacgac cagcgcgaac ggcagcatga tacaaaacag ataaacggta
    10081 cgatgcaaaa tcagggtgta ggcaaaggga attggcgtgt aggcaatgcg ctcgcagcct
    10141 gccagaaccg ctgaaatatc gttaagccga tcgttgaggc taataaacag gatatctgaa
    10201 agttgtccat tgcgtcgctg aaccgccaac cactccccca ttattaacaa gatgcggtta
    10261 gctggagagt tcgaagccag tacacgctga agatcttcag tcttgagata atgagccagc
    10321 acttccacct gtggctgttt gcgtaatgtc atgcgtaaac agtgggcgaa ggcgatttgc
    10381 agccgggcaa actcccttac acttgccgaa tccggcaatg tcgtttttac ctcgcgcagt
    10441 aacgaccgtg aggcaatcat caactgcccc caaagtttgc gcgcttcaac gtaacgggcg
    10501 taaccggcat tattacgaaa accaagaaaa atggcgatgg cgacaccgag aatgctgaac
    10561 ggtgcgaggg tgaatttgat gcccagatgc gtgtaccagg gcagcatgaa aataacagcg
    10621 atagaaaaga gaaaattgag tagtaagcgc gaggatatct tggataatac tgagccatgc
    10681 cagacaaaaa tacggcgcag ccagtgttgt tgtggacgaa caatcatggt tatcttcagg
    10741 cgtggaaaag tcgccctatt aaacgtgatt acgatcacat tctcaagaca ctacttacaa
    10801 attacctaca aaattacaac attaacaggt gctgcatgtg gcacctgtta atgataaa
//
LOCUS       DAZFAB010000135        10789 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZFAB010000135 DAZFAB010000000
VERSION     DAZFAB010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10789
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.135"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..773
                     /locus_tag="ACHJWA_004164"
     CDS             <1..773
                     /locus_tag="ACHJWA_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4310650.1"
                     /translation="GWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKLEKTLLIN
                     QLASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGF
                     TASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIDIGLSGLLSPVLAIV
                     ALCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLLGAVGGFIAPILRVKAETLF
                     ASDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            799..1593
                     /gene="yfaU"
                     /locus_tag="ACHJWA_004165"
     CDS             799..1593
                     /gene="yfaU"
                     /locus_tag="ACHJWA_004165"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HFO4310651.1"
                     /translation="MINHPFKERLRKGEVQIGLWLSSTTAYMAEIAATSGYDWLLIDG
                     EHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKPLIKQVLDIGAQTLLIPMVDTADQA
                     RQVVSATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDNLDE
                     ILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAVAPD
                     MAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(1634..2560)
                     /gene="rpnE"
                     /locus_tag="ACHJWA_004166"
     CDS             complement(1634..2560)
                     /gene="rpnE"
                     /locus_tag="ACHJWA_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFO4310652.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLEGLQEGMHEQAI
                     KIALRMLEQGFDRDLVLAATQLSEADLAANNH"
     gene            complement(2753..3943)
                     /gene="glpC"
                     /locus_tag="ACHJWA_004167"
     CDS             complement(2753..3943)
                     /gene="glpC"
                     /locus_tag="ACHJWA_004167"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFO4310653.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSVAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(3940..5199)
                     /gene="glpB"
                     /locus_tag="ACHJWA_004168"
     CDS             complement(3940..5199)
                     /gene="glpB"
                     /locus_tag="ACHJWA_004168"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFO4310654.1"
                     /translation="MRFDTIIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQAVTDIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELPHQRITPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLAVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDQTLRPSQAGQTIENLFTIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(5189..6817)
                     /gene="glpA"
                     /locus_tag="ACHJWA_004169"
     CDS             complement(5189..6817)
                     /gene="glpA"
                     /locus_tag="ACHJWA_004169"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFO4310655.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            7090..8448
                     /gene="glpT"
                     /locus_tag="ACHJWA_004170"
     CDS             7090..8448
                     /gene="glpT"
                     /locus_tag="ACHJWA_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFO4310656.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHQELLQKRN
                     GG"
     gene            8453..9529
                     /gene="glpQ"
                     /locus_tag="ACHJWA_004171"
     CDS             8453..9529
                     /gene="glpQ"
                     /locus_tag="ACHJWA_004171"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO4310657.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(9753..10787)
                     /locus_tag="ACHJWA_004172"
     CDS             complement(9753..10787)
                     /locus_tag="ACHJWA_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310658.1"
                     /translation="MLQNNKLQPTEIYKESQQTAKLEFFCDEFLKISQSRYGLSTSTD
                     SIANLLAFFTKASDAIDSIKTQKIDVHSYGFVPIRHFVEHVITYKNEFAADYEPYTLT
                     FTRGENNEGILSVESKEGFISQRTINLNEYETAINIINEHVIKENIHNTVQSPTEKYI
                     SKINSSDKPYKTSSKESIKSQLYSDQRNIQTCYYILRKNTEWYKYASSEERYDKFKNS
                     SKEIKNTYKQIVQAQKKLNQMKYINKLSGELIDITDKKLAPLINDSFSYTRDFFAYSK
                     QEKNIFTFDNSKFVDPKEKEGLMVQHSNGQLVITGKYCPEGVQTAFTQEQYDKLIRYI
                     NIFFTFPKLE"
BASE COUNT         2507 a         2561 c         2871 g         2850 t
ORIGIN      
        1 ccggctggtt ctggatgttt gtgattgaag gattgttggc agtcggcgct ggggtattca
       61 cattcttttg gcttgatgac acaccggagc aggcacgttt tctgagtaaa ctagaaaaaa
      121 cgttgcttat caatcaactg gcaagtgaag aacaacagaa agtgacttct cggctgagcg
      181 atgcgctgcg taatgggcga gtctggcaac tggcgattat ctacctgacc attcaggtgg
      241 cggtttacgg attaattttc ttcctgccga cccaggttgc ggcattgctg ggaacaaaag
      301 tgggctttac agcgtcggtg gtcaccgcca ttccgtgggt tgcggccttg tttgggacct
      361 ggcttattcc gcgctattcc gataaaaccg gcgaacggcg taatgtcgca gcgctgacat
      421 tactggcggc gggcattgat attggtctgt ccgggctgct ttctccagta ctggcgatcg
      481 tagcgctgtg tgttgcagcc atcgggttta ttgccgtgca gccggtgttc tggacgatgc
      541 cgacacaact gctttccggt acggcgctgg ctgcggggat tggttttgta aacctgcttg
      601 gtgcagtggg cgggtttatt gccccgatcc tgcgcgtgaa agcagaaacg ttatttgcca
      661 gcgatgcggc gggattactg acgctggcag cggtggcggt catcggttcg ctgattattt
      721 tcactctgcg tgtaaatcgc accgttgcgc agaccgacgt ggcacatcat taaataggtt
      781 aaggaacacg atgaacgcat tattaaccat ccctttaaag aacgtttacg caagggcgaa
      841 gtgcaaattg gtctgtggtt aagctcaacg actgcctata tggcagaaat tgccgctact
      901 tccggttatg actggttgct gattgacggg gagcatgcgc caaacaccat tcaggatctt
      961 tatcaccagc tacaggcggt agccccctat gccagccaac ccgtgatccg tccggtggaa
     1021 ggcagtaaac cgctgattaa acaagtcctg gatattggcg cgcaaactct actgatcccg
     1081 atggtcgata ctgccgatca ggcacgtcag gtggtgtctg ccacgcgcta tcctccctac
     1141 ggtgaacgtg gtgttggggc cagtgtagca cgggctgcgc gctggggacg cattgagaat
     1201 tacatggcgc aagtcaacga ttcgctttgt ctgttagtgc aggtggaaag taaaacggca
     1261 ctggataacc tggacgagat cctcgacgtc gaagggattg atggcgtgtt tattggacct
     1321 gcggatcttt ctgcgtcgtt gggctacccg gataacgccg ggcacccgga agtgcagcga
     1381 attattgaaa ccagcattcg gcggatccgt gctgcgggta aagcggctgg ttttctggct
     1441 gtggctcctg atatggcgca gcaatgcctg gcgtggggag cgaactttgt cgctgttggc
     1501 gttgacacga tgctctacag cgatgccctg gatcaacgac tggcgatgtt taaatcaggc
     1561 aaaaatgggc cacgcataaa aggtagttat tgatatcaaa ggcccatggg gatcggctgt
     1621 gggactgtgt taattagtgg ttattcgctg ccagatcggc ttcgcttagc tgggtggccg
     1681 cgagcaccag gtcacgatca aagccctgtt ccagcatgcg caaggcaatt ttaatagctt
     1741 gttcatgcat gccttcctgc aaaccttcta atttaccttc ctgccaacca atttgatgtc
     1801 cttcctgccg tagccgttca gcaatagtca ttaatctctc cttttgtagt ggtgaacgtt
     1861 cggcaatctc ctgaataaaa cgggtgaaac gggaggtatc gccgcattgc agcagataat
     1921 taaacagtgt ttgtagctgg ctgtcattag tgaagcctct aaccaaaagc gtggtgatcc
     1981 tgtcgaccat accgattaaa tcgcggtcgc gaatatgctt ttgaatcagt tcaagcagag
     2041 ctatacgccg atgttgcatg atctcatcgt caggtacgat ggtaatatcc accaacggaa
     2101 aagcttcggt gtacaactgc cgggcaagtt gcggatcgtc aaactcatcc agccagttga
     2161 gtgagtacgg gtagggcgag gtttcgccat gataaaacag caacggcacc accagcggga
     2221 ctctgtcata gcctttgtcc aggtgacgct gcatggcggc agtggcatag cgcattagcc
     2281 gaaaagccat attcttttct gcagagcttt gatgttcaat cacgcagtag atatagccgt
     2341 caccgtcgct ggtttccacg gaccacaaaa catccgagta gtaagcgcgt aaactttttt
     2401 caataaaact agtgggttcc aggcgtaagg tttgcaggtt gcaaagcttg cgcagtggtt
     2461 ctggtaaatg tatttcgaga aaatcccgtg cggtttcggg tgtgaacata aaggttttaa
     2521 atactgcatc atgcggggag gaggttgttg attcggtcat ggttgtccgt cacctgatat
     2581 ttatgatgac gcgacattac ggttatccca ggcctttgac atctgccagt ttcagccttt
     2641 gcgagacgtt ttcagaggaa ttttcactgc gttgcaacgc atacattggg cacggaatcg
     2701 tgtacgcaaa aatgcccggt aaccacatgc ttaccgggca tcagaaagga gtttaagcca
     2761 gcgcctgggc cagtagcgta atcggatgtt cgcagcgaag acttgtggac atctcaatct
     2821 gccatttaca ggtttcgcag tcggtgacca ccagatctgc gccgctttct tctatctggc
     2881 ggaacagtgg tgcgccgatg gcttgtgagg tggggtagtt ctcttttttg aaaccgtaag
     2941 tacccgcaat accgcagcac tgggaatcca gcaccgttaa ctcaagcccc gggatgttac
     3001 gcaacagctc cagggtgtag agcgtccagc ccattttttc catatggcac ggagtgtgat
     3061 aaaccacttt cagcggcagc ggtttcagcg gtaacgtttt gccttcgtcc agcttgcgcc
     3121 acagccagcg ggtcgccagt tcgatatgat cgcgcaagcc tttgttgtcg acattcagca
     3181 cttccgggta ttcgtcgcgc agggcaaatg tacaggttga ggaggtggca atcaccggaa
     3241 tgccttttac tcccacagct tcgcggatcg actctacatt cgtaattgcc tgtttgcgtg
     3301 ctttatcggt aaagccgttg gcgatcagcg gtacgccgca gcatttttct ttgctgagca
     3361 gttgtacacc ggtacccatt gcgttgagca ctttaattaa atctttacct aactgcggat
     3421 ggttgtagtt aacgaagcag ccgtgaaaga aagcgacctg gtctttatac tgcgcttgct
     3481 gagccgccac gctgcgatac cagcgacgaa acgtgccgaa ggagtatttc ggtagcgtgc
     3541 ggcgatgatc gatttttaac gccgcatcaa gcaactgccg caccggtttc agcgaggtag
     3601 cggtgttgac gattggtgcg aacggcgtgg aaacgctacc catcaggtcg gtatggctta
     3661 aaacaaaatt gcgcagcgac gggcgcgtgg tgtcatattt cgcccgtgcg cgctggatga
     3721 tatcgccaat cttcacatcg gacgggcagg cgacttcgca acgtttgcag ttgatgcaat
     3781 atttcagtgc ctcgtcatac agtgcgccat cttttaaacg cagacgctcg ccatccggcc
     3841 cggcttgttt tggccctgga taaccgggat tcacccggct cactgggcag gcggtggtgc
     3901 agacggtgca cttaatgcag ttttcgaagc tggtgtcatt cattgttggc ctcctgcgcg
     3961 ttgggcaatc tgttgagcgg catgtaaagc actgacggca caaacaccgc cgccgcatcc
     4021 ctgggcgatg ggatcaaatc cgcccagcac cgaaccgatg gtaaacaggt tttcgatggt
     4081 ttgccctgcc tgcgacgggc gtagcgtctg atcagtggtt acaccgaact gctgccacgg
     4141 ttgcggcgca aaaaaatctc ccttatacca ttcaccccgc gtggcggttt gtagcacatc
     4201 aaggccgaga atcggctctc gaatgccgtt acgttccgct accagtccgc cactaaagaa
     4261 actgccgctg gcgagaaccg cgaaacgtgg acgcagcggg atatcggcgt gattgcgggt
     4321 ccagatttca ttcactacgc catttttaca ggtcactttt ttcacttcat cgcccggcat
     4381 ccacacgcca ccctgacgca caaactggcg ctgtaactgg ttttgcagac gaatgcccag
     4441 cacggaaggc ggcagcgttg gcaaaagcat cagtgaacaa ggtagttttt cattcaacca
     4501 acgccacagt ttgtcatcag ccaggccgaa gcaggcgggc atcaggatca tttcgcaggt
     4561 attggcgaca ggaataagcg catcaagtaa cagcggccag ttttcttcat tatcaaggaa
     4621 acgggcgata ttcaccgcgc ggaactcggt ggcgttattg cgcaggacat ccagttccgg
     4681 cagctctatt tctgcggttt caacggcaag gtcgagttca cgcaacgaag ctgccgcaag
     4741 gtgcgcctga aaatccatca gtccgctaat tcccactaca catattttct tcgcgggcag
     4801 cggccagacg gggacttctg gcgaacttag ccaggtagag cgcagagtgc cgagcggcgt
     4861 aatccgctgg tgaggcagtt ctacgctgcc ctgcaattgc gcaccgcttt cagcgattaa
     4921 tgcctgcgcc tggcaagcga gatcgagcac gcgttgtggc ccgagaagag agtaaggatg
     4981 tgcaggtgcc tgctgacgca aagattccag tccactgtga atgtctgtca cagcttgacc
     5041 atctggcaga tggctcagca aatccagcga accggatgag aaatgcagcg cgctttgacc
     5101 acgcgtgaca atggcacagc gcaggccgtg tttttgcagc tgcaggccac agagtaatcc
     5161 ggcgaggccg ccgcccataa tgatagtatc aaagcgcatc tttctgctcc ttctccagac
     5221 cacacagtcc ctgataaacc cagcgggtaa attcgctttc gcgcagtgca tctccccagg
     5281 cgatgggttg cacgcctttc cagcgttcgt taaggaaggt ggaaagttgc tcgatagatt
     5341 gcgcggacgt cgtgacgtta aaacgttgca gcagtccagc agcgcggcag gcgcagagtt
     5401 cgccctggca ggtacccatc cccacacggg tacgacggcg taaatccagc aggctattaa
     5461 cgtttaaatt ttctaccgcg tactgcactt cacccgcagt taccgcttcg cactcacata
     5521 ccaggctacg gtgcagacgg ccttcgctca gccaggcagg cgtgcgatcg ccatgacgat
     5581 aaaccgcaga accgcgcagc ggggcaggca gggagatgac tttacgcaag gtaacttcag
     5641 cgggttcctg tgaacccgga agtgccagat cggcggtcgt acaggggcgc gtgttgccca
     5701 gtttgcggca taccgcgtcg gtagcccatt cagccatcag ccgataggtc atcagtttgc
     5761 caccggtgat ggtgataaat ccgtccagac catcgcgttc agcatggtcg agcagcacga
     5821 tgccacggct gacgttacgt ccgctcgggt cgtcatcgct ggcaaccagc gggcgcacgc
     5881 cagaataggc ccgcaaaatg cgcgttttcg ccatcacggg ggccagtttt tccccttcac
     5941 gcagcagaat atcaacctct tctgccgtca ctcgattatc gtcaatctcg ttgtaatcaa
     6001 tacgtaaaga ggtggtgcca atcagcgaaa tggtatcgcc aggcaccaga atatcggcgt
     6061 cggaaggttt acggcagcgg ttgatcacat gctgattaat gcggtgatcc atgatcagca
     6121 gcgatccttt cgccgggaac atgcgaatgc gcagatcggc atattcggca atgtgttgcc
     6181 cccagatccc agcggcatta accacgacag gtgcatgaag ggcctgagtt tcgccggtga
     6241 gatggttacg tacacgaaca ccgcacaccg tcgcgccttc acgaatcagc cccgtgactt
     6301 catgagcggt aaggataaca gcaccgtgtt ctttggcatc cagcatgttt gctgcggtca
     6361 gacgaaatgg atcaacggtg ccatccggaa ctttcaccgc gccaatcagt gccgggttaa
     6421 cggcaggttc gataatgcgc gcttgctgcg ggtctatagc ttctgcgctg atccctgctt
     6481 cttcgcaggc gcgaataaaa gtggcctgga aggagaggtc atcttccggc agggtgataa
     6541 acaggccgtt ggttggttca acgcagtgac gtgcaatgcg tttcaggatc tggttttcac
     6601 taatgcattc gcgggccgat tccgcatcgg ttaccgcata gcgcgcaccg ctgtgcagca
     6661 ggccgtggtt acgcccggtg gcaccggttg cgatgtcgtg gcgctcaacc aaaatcacgc
     6721 gcagcccgcg cagggcacag tcgcgggcaa ttccggctcc cgttgcgccg ccgccaatga
     6781 taatcacgtc acttgattgc gagtcgcgag ttttcattgt tatccctctg aagttcgttt
     6841 tttaccattt agccatagta aaaacatgag ttgtttgatt tcgcgcatat tcgctcataa
     6901 ttcgaaagtg agacgtgatt tcatgcgtca ttttgaacat tttgtaaatc ttatttaata
     6961 atgtgtgcgg caattcacat ttaatttatg aatgttttct taacatcgcg gcaactcaag
     7021 aaacggcagg ttctctcact gaatcaggct gttaatcata aataagacca cgggccacgg
     7081 aggctttcaa tgttgagtat ttttaaacca gcgccacaca aagcgcgctt acctgccgcg
     7141 gagatcgatc cgacttatcg tcgattgcgc tggcaaattt tcctggggat attctttggc
     7201 tatgctgctt actatttggt tcgtaagaac tttgcgctcg ctatgcctta cctggtggag
     7261 cagggattct cacgcggtga tttaggtttt gcgctttctg ggatctcgat cgcctatgga
     7321 ttttcgaaat tcatcatggg ttcggtatcg gatcgctcga atccgcgcgt tttcctgccc
     7381 gcaggtttga ttctggcggc ggcagtgatg ttgtttatgg gctttgtgcc atgggcgacg
     7441 tcgagcattg cggtgatgtt tgtactgttg ttcctctgcg gttggttcca ggggatgggg
     7501 tggccgccat gcggtcgtac tatggtgcac tggtggtcgc agaaagaacg tggcggcatt
     7561 gtgtcagtgt ggaactgtgc gcacaacgtc ggtggtggta ttccgccgct gctgttcctg
     7621 ctggggatgg cctggttcaa tgactggcac gcggcgctct atatgcccgc tttctgcgcc
     7681 attctggtgg cattattcgc ctttgcgatg atgcgcgata ccccgcaatc ctgtggcttg
     7741 ccgccgatcg aagagtacaa aaatgattat ccggacgact ataacgaaaa agcggaacag
     7801 gagctgaccg cgaagcaaat cttcatgcag tacgtgctgc cgaacaaact gttgtggtat
     7861 atcgccatcg ccaacgtgtt cgtttatctg ctgcgttacg gcatcctcga ctggtcaccg
     7921 acttatctga aagaggttaa gcatttcgcg ctggataaat cctcctgggc ctacttcttc
     7981 tatgagtatg cgggtattcc gggcaccctg ctgtgcggct ggatgtcgga taaagtcttc
     8041 cgtggcaacc gtggggcaac cggcgttttc tttatgacac tggtgaccat cgcgactatc
     8101 gtttactgga tgaacccggc aggtaaccca accgtcgata tgatttgtat gattgttatc
     8161 ggcttcctga tctacggccc tgtgatgctg atcggtctgc atgcgctgga actggcaccg
     8221 aaaaaagcgg caggaacggc agcgggcttt accggtctgt ttggttacct gggcggttcg
     8281 gtggcggcga gcgcgattgt tggctacacc gtggacttct tcggctggga tggcggcttt
     8341 atggtaatga ttggcggcag cattctagcg gttatcttgt tgattgttgt gatgattggc
     8401 gaaaaacgtc gccatcagga attactgcaa aaacgcaacg gaggctaatg gcatgaaatt
     8461 gacgctgaaa aaccttagca tggcgatcat gatgagcact atagtcatgg gaagcagtgc
     8521 aatggcggcg gacagcaacg aaaaaatagt catcgcccat cgcggtgcca gtggatattt
     8581 gccggagcat acgctgccag caaaagcgat ggcgtatgcg cagggagcgg attatctgga
     8641 acaggatttg gtgatgacca aagacgacca tctggttgtt ctgcatgacc attacctcga
     8701 tcgtgttact gatgttgccg atcgtttccc ggatcgggcg cgcaaagacg gtcgttacta
     8761 cgcgatagat ttcacgctgg atgaaattaa gtcgttgaaa tttaccgaag gtttcgatat
     8821 tgaaaacggt aaaaaagtgc agacttatcc agggcgtttc ccaatgggta agtccgactt
     8881 ccgggtgcac acctttgaag aagagattga atttgttcag gggttaaatc actctaccgg
     8941 gaaaaatatc ggtatctatc cagaaatcaa agcgccgtgg ttccatcatc aggaagggaa
     9001 ggatattgcg gcaaaaacgc tggaagtgct gaagaaatat ggttacaccg gtaaagacga
     9061 taaagtttat ttgcaatgtt ttgatgctga tgagctgaag cgtattaaga atgagctgga
     9121 acccaaaatg ggcatggatc tcaatctggt acagctgatt gcctataccg actggaatga
     9181 aacgcagcag aaacagccgg acggaagctg ggttaattac aactacgact ggatgtttaa
     9241 gccgggtgcc atgaaacagg tggcggaata tgcagatggt attggtccgg attaccatat
     9301 gttgattgag gagacatcgc agccgggtaa tatcaaactc actggcatgg tgcaagatgc
     9361 tcagcagaac aagctggtag tgcatcctta taccgtgcgg tcagataaac tgcctgaata
     9421 cacaactgat gtgaatcagt tatatgatgc tctgtataac aaagcgggtg taaatgggtt
     9481 gtttactgat ttccctgata aagcggttaa attccttaat aaagagtaat gctgctactg
     9541 caggatggcg ttcatgcgcc atcctctgct aaagcgaaat aatgctacta ccggttattc
     9601 ttttcattaa ccggtgcgtc ctgtaaaaaa gatgccaaca taattttcgt aaaactcatt
     9661 cgttaaaaaa gctaacactg ggcattgtta ttctttaaac agtatgaata ggtctcaata
     9721 ataaattgta atgcagttta tactgtaaag tattattcta atttcggaaa ggtaaagaaa
     9781 atattaatgt atcttattaa tttatcatat tgttcttggg tgaatgctgt ttgcacacct
     9841 tcagggcaat atttaccagt aataaccagt tgcccattgc tgtgttgtac cattaaacct
     9901 tctttttcct ttggatctac aaacttagaa ttatcaaatg tgaagatatt tttttcctgt
     9961 tttgagtaag caaagaaatc tcgtgtatag ctgaaactat catttattaa tggtgctaac
    10021 tttttgtcgg tgatatcgat aagctcacca cttaatttat tgatgtattt catttggttc
    10081 aattttttct gcgcttgtac aatttgcttg taggtatttt taatttcctt agaagaattc
    10141 ttgaatttat catagcgttc ctcggaggat gcgtatttgt accattctgt attttttctc
    10201 agaatgtagt agcaggtctg tatatttctt tgatcgctgt aaagttgact tttaatactt
    10261 tccttactgc tggttttata aggtttatca gagctgttta tttttgaaat gtatttttct
    10321 gtcggtgact ggaccgtgtt gtggatattt tcttttataa catgttcatt gatgatgtta
    10381 attgcagttt catattcatt taagtttatt gttctttgtg aaataaaccc ttctttgctt
    10441 tcgacactca aaataccttc gttgttttca cctcgggtaa aggtaagggt atagggttca
    10501 taatccgctg caaattcatt tttatacgtt atgacatgtt cgacaaaatg tcttattggt
    10561 acgaaaccat aactgtgaac gtcaattttt tgggtcttga tactatcaat tgcatctgag
    10621 gctttcgtga aaaaagccaa taaattagct atgctgtctg ttgaggttga taacccgtat
    10681 ctactttgag atatttttag aaactcatcg caaaaaaact caagtttggc ggtttgttgt
    10741 gattctttgt aaatttctgt tggttgcaat ttattatttt gtaaaatat
//
LOCUS       DAZFAB010000136        10390 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZFAB010000136 DAZFAB010000000
VERSION     DAZFAB010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.136"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            225..1118
                     /locus_tag="ACHJWA_004173"
     CDS             225..1118
                     /locus_tag="ACHJWA_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310659.1"
                     /translation="MVANINLIKQESYSVVNLEKQLMSNESPKQSLLNDFSHRLKMEG
                     EEQLFHCVNELKYGLSSNNEYDLFKNRETSLWKRKVPESAALAMTGTVYKLFGSRLLN
                     ARNQLDKNLLEMAMQMVYGKDMVTKSGDILIDIQLNKDGSLQSKHYTFDVGEVINSYN
                     IDLFVNASSINQTYLHDKNPGDVISLYGTLDNIPLVVKECTGKNHQINVKAKLPQERS
                     EKVEDMCERMRGGISMFNHTTKTAGNIEHNLQLSFLVDSRPSITNKYSAKDVAPDILV
                     LPVNVYHADFKIKINNAVEKT"
     gene            1125..2039
                     /locus_tag="ACHJWA_004174"
     CDS             1125..2039
                     /locus_tag="ACHJWA_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO4310660.1"
                     /translation="MLCAVQKNLHAARKVLSGIIGNVPKNNKPKTIIFDLRFLSAIKQ
                     KVFLGSEQPLFVKHTAMMREACKELPQLVEYVPLACEAHSHRSVALAKTVAAPIMATG
                     VGAALMYATSSGPYYAQSLSGSPDIEMIKKRMAELFNQSDTSVRNKYRPAFDKWNKLV
                     DKLNHERNTVPFACLTTIIATAINETPEGDTNVAVVMGCKSAKDRTISIVLGNSMLQT
                     LFEKRLADDGEIENLFDQQGYFSCDSLTAEEVMMLKDLFDIRVLHLSNKFNVGLQGNI
                     NTDVLQDSFFKNVDFIHESNSYTVGMGV"
     gene            2733..2951
                     /locus_tag="ACHJWA_004175"
     CDS             2733..2951
                     /locus_tag="ACHJWA_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFO4310661.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            3168..3410
                     /locus_tag="ACHJWA_004176"
     CDS             3168..3410
                     /locus_tag="ACHJWA_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFO4310662.1"
                     /translation="MNSLAGIDMGRIILDLSNEVIKQLDDLEVQRNLPRTELLREAVD
                     QYLVNQSQTVRTSAFGIWQGCEEDGVEYQRKLREEW"
     gene            complement(3740..4669)
                     /gene="fdhE"
                     /locus_tag="ACHJWA_004177"
     CDS             complement(3740..4669)
                     /gene="fdhE"
                     /locus_tag="ACHJWA_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFO4310663.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(4666..5301)
                     /gene="fdoI"
                     /locus_tag="ACHJWA_004178"
     CDS             complement(4666..5301)
                     /gene="fdoI"
                     /locus_tag="ACHJWA_004178"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO4310664.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(5298..6200)
                     /gene="fdoH"
                     /locus_tag="ACHJWA_004179"
     CDS             complement(5298..6200)
                     /gene="fdoH"
                     /locus_tag="ACHJWA_004179"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFO4310665.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNHADEEENNLHEEKDEERK"
     gene            complement(6213..9263)
                     /gene="fdnG"
                     /locus_tag="ACHJWA_004180"
     CDS             complement(6213..9263)
                     /gene="fdnG"
                     /locus_tag="ACHJWA_004180"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(8676..8678),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO4310666.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQGNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVISCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            9457..10290
                     /gene="fdhD"
                     /locus_tag="ACHJWA_004181"
     CDS             9457..10290
                     /gene="fdhD"
                     /locus_tag="ACHJWA_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFO4310667.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         2645 a         2471 c         2623 g         2651 t
ORIGIN      
        1 taatgcttaa ttcatcatct tagttcaggt ttgagcgatt tttgatagtc aaaaccccct
       61 ctggtgccat agttttctca taaatacttt aaaaatctat agctaacaaa cattatgcaa
      121 agataatggg ctggcatcat tgtcagtatc attcatttac tgctctggat gttataaata
      181 attatgtagc atgtttgctc ttaaaccgtg ggaaggaggc tgtaatggtt gccaatatta
      241 atctcattaa acaagaaagt tattctgttg ttaatttaga aaaacaacta atgagtaatg
      301 aatcaccgaa gcaatcgcta ttaaatgatt tttcacatcg ccttaaaatg gaaggtgagg
      361 aacaactgtt ccattgtgtt aacgaactga agtatggttt gtcgagtaat aatgaatatg
      421 acctgtttaa aaatagagaa acctcactgt ggaaacgcaa agtaccagaa agcgcagcgc
      481 tggcaatgac gggaacagta tataaattat ttggtagtcg cttactcaac gcccgcaatc
      541 agttagataa aaatctcctc gaaatggcta tgcaaatggt ttatggcaaa gatatggtga
      601 caaagtccgg ggatatattg attgatatac aattaaataa ggatggcagc ctccagagta
      661 agcactacac atttgatgta ggtgaagtta taaacagtta taatattgac ctctttgtga
      721 atgcttcgag tattaatcaa acttacttac atgataaaaa tcctggtgat gttattagcc
      781 tgtatggcac tttagataac attcctcttg tagttaagga atgcacgggt aaaaatcatc
      841 aaattaacgt taaagccaaa ttgccacaag aacgaagtga aaaagttgag gatatgtgtg
      901 aaagaatgag aggtggcatc tctatgttta atcacaccac aaaaacagct ggtaatatag
      961 agcataattt gcagctaagt tttctcgtgg attcacggcc gtcgatcacc aataaatact
     1021 cagccaaaga tgtagcgcca gacatacttg tattgcccgt caacgtatat catgcggatt
     1081 ttaaaattaa aatcaataat gcagtagaga agacttagtc aattgtgtta tgtgctgtgc
     1141 aaaaaaatct ccatgcagca aggaaggttc ttagcgggat aataggtaat gtaccaaaaa
     1201 ataataaacc aaaaacaatc atttttgatt tacgttttct tagtgcaatt aagcagaaag
     1261 tttttttggg cagtgaacaa ccgttgtttg taaagcatac cgcaatgatg agggaggcat
     1321 gtaaggaatt accgcagcta gtggagtatg ttcctttagc ctgtgaagcg catagccatc
     1381 gcagcgtcgc ccttgccaaa accgtggctg cacccatcat ggcgacaggt gtgggcgcgg
     1441 ctttaatgta tgcaacatca tcaggaccgt attatgcgca aagtctttct ggttcgccgg
     1501 atatcgagat gataaaaaaa cgcatggctg aattgtttaa ccagtcggat acttctgtgc
     1561 gcaacaaata tcgcccggct tttgataaat ggaataaact ggtagataaa ttaaatcacg
     1621 aaagaaatac agttccgttt gcgtgtttga ccaccattat agcgacagcg ataaatgaaa
     1681 ctcctgaggg ggatacgaat gttgccgttg tgatggggtg taaatctgcg aaagatcgca
     1741 cgatatccat tgtccttggc aactcgatgt tgcaaacact ctttgaaaaa cgcctcgccg
     1801 atgatggaga aatagaaaac ctttttgatc aacagggata ttttagttgc gattcattaa
     1861 cagccgaaga agtaatgatg ttaaaagacc tttttgatat tcgagttctt catctctcta
     1921 acaaatttaa tgttgggttg caaggtaata tcaacacgga tgttttacaa gattcgtttt
     1981 ttaaaaatgt cgatttcatt catgaatcta actcgtatac cgtagggatg ggagtctgaa
     2041 gagtaaaact cattaggatt atcagaattc ggccaatgca ttgcaaaaat gaaatacaaa
     2101 gagagatgtg tgcaggcgct tagccgcgag gggcatggcg aagccataca gaaagcaccg
     2161 gcattcagaa ctcctgtgaa agcggcatag tgccgctgct caggagtttt gttatgaccc
     2221 gtatcgtgct gaacgttgcc accctatgat gcgattaaaa aaactcaccg tcgagccgat
     2281 gattgacgtc aatgctatcg gatgatgccg ggggtgttcc ggaaggaatt ccttgtttcc
     2341 cgaactgtta ccaacaaaat tataacgatg agcaagtaaa gaccggttgg tggcagtact
     2401 gaccggtctg tgatggaaga ttaatttcac cttaaatcct ggtgctgtta ggatgctgac
     2461 gtttttcggg tacaccattc agattatgca aataaagatt caatgaaaaa ataaatatcc
     2521 ctgaatataa tatattcgta gcacccataa ctcatcctgg gcgataacta aaccattttt
     2581 gagtaacaaa tgaaacaact gcaacgctct tattatttct ggaaaacgtc tcgcaaaaac
     2641 aaaacaacat gcaaaaatcc gctttcgtta attaattttt cggcgtaatt ttcgcacata
     2701 cattaatgag taatatatgt aaggtggtgg taatggcgat gaatacggtt tttcttcatt
     2761 tatcagaaga ggcaattaaa cggctgaaca agcttcgggg ctggcgtaag gtttcacgat
     2821 ctgcaatttt acgcgaagcg gtagagcaat atctggaacg tcagcaattc ccggtgcgta
     2881 aagcaaaggg aggcaggcaa aagggggagg tagttggtgt tgatgatcag tgtaaggagc
     2941 ataaggaatg acaaaaattt ttcattcttg aatataaaaa acagatgccc ttattctggt
     3001 attaatagaa ggctgtttta cttgaactta taataactgc aactgttaca tcatatctgg
     3061 aaaacgcctc gcaaaaataa aaaatgatgc gtaaatgagc tttctttaaa gattcgacaa
     3121 gggtactttt ctctccatag atatcaaatg atattggtca aatatttatg aactctctgg
     3181 caggtattga tatgggcaga attatactcg atttatcgaa tgaggtgatt aagcaacttg
     3241 atgatcttga ggtgcagcgt aatcttcctc gtacggagct attacgggaa gcggtagatc
     3301 aatacttggt aaatcaatcg caaacagtaa gaaccagtgc ttttggcata tggcaagggt
     3361 gtgaggaaga tggtgtcgaa taccagcgta agctgcgcga ggaatggtaa tgggaaaaaa
     3421 tgatatcaat cagattgctg acaacgtgcg cgttgttcat gccggatgcg gcgtgaacgc
     3481 cttacccggc ctacaaagtc gtataaattc aatgtattgc agtttattgg taggcctaat
     3541 aagcgcggcg catcaggcaa tcttacgctt atcatcagtc tcatacccat aaccttattg
     3601 atgtgggggt gggtaaattg aggcaaaaca ggcactggcg gcatagcttg aggtggtaac
     3661 gcggaatacg agagatggtg tcgtcacacc ctatgagctg taatccgggc gaccagcgcc
     3721 acccggaaaa cgtcagagat tactcccctt cacccggaaa caggaacggg ttgatggaac
     3781 tgcgggcata gccttcttgc tccattcgcg cgtccagtac cagagaggcg aggtcatctg
     3841 ccacggcttc aactttcggt tctttttcct gatagagaat tttcaggtaa gtgccgcagt
     3901 catcgcagct ttcggcttta atcgcggcct gttcgtcatc cagcgaccag taatgcagtt
     3961 tgccgctctg ctcgcagttg ctgcatttta cgcgcacaac gtgccattca gtttcacaca
     4021 ggttgcagtg caggtaacgt agcccctgag tggtgccaat ttgcaccatg ctggacaccg
     4081 gcatagaacc acaaaccggg caatattgac gttgctcgcc gtattcagcg cgggctttgc
     4141 cggggatcag attggccatc tgcgcccagt agagcgacag tgcagcccag ataaacggcg
     4201 ctttatcgct gctgacggac gagaaatcag aggcaaacag tgcgctggcc atatcttcca
     4261 gctcctgagt cgatgccttc tccagattct caatcactgc cagcgccggg ccgctcattt
     4321 caggtttcag ctcagcaatc agcgccatca gcagcttttg ccagtgctta tcacgcggca
     4381 gaacgtgaat atccagcggg ggcttgcctt gtgcgctggc ttctttaatg cgcgcagtca
     4441 gatccatctc cagcggatgg tcgtacagca ccacttcctg ggcgtgggcg ataagcgcag
     4501 caaagcgcag gtaatcaccc agcggattat tttctgccag ctcgcgcaga cgctcggcgc
     4561 ggcggttgta taaattcttg agccgaggga acaataacgg cggaatcata tccgccgtac
     4621 gtttctcgct cgaacccagc tcatcttgcg ggattatgcg aatactcatt cagctttctt
     4681 ttccgttgtc ttgcggacct cacggtacca gcgcgggtga tgtttcttcg cccaagcgct
     4741 ggtaacccat ccttccacca tggcggtaat cgtgcctttc acccaaaggg cggcgtagat
     4801 atgcaccatg ataaccacaa ttaacgccac tgcggcaaat gaatgcagca ttaacgcgaa
     4861 tcggatcacc gggattgaga aagcaggcgc aaaataagga cgccagataa tcacgccgct
     4921 caccagcagc agaaccagga aaataatcgc cgcccagaaa acgcatttct gaccgaagtt
     4981 ataacgcccg gtgtcaccta cttcctcgtt gacgacgatc ttacgaatat tcttcgccca
     5041 aaagatatca tcccgattga ttaggttgtg atgccagtaa cggaaaaaca tgatgatgaa
     5101 cgaggcaaac ataaccacgc cgacaaacgg gtgcagaatt cgcgccagct gcggtgtgcc
     5161 catgatttgc atcaaccagt tgaaggacgg gaacaaaaag cccagcccgc tcaccgccgc
     5221 caggatgaag cagaaggcgg tgatccagtg gttgatacgt tccggcgcgg tgtagcgcac
     5281 gatggtgtca cgtcgtttca tttgcgctcc tcgtctttct cttcgtgcag attattctct
     5341 tcctcatccg catggttcgg accgacaccc acgtagtgga agatactggc tgcgaaggta
     5401 gccgcaaagc caacagctgc gagcggtttc cagatgcctt tccagaattt cacggtttcg
     5461 ctgatttccg ggttctccgg caagccatga tacagatttg gcttgtcagc atggtgcagc
     5521 acgtacataa cgtgtgtacc accgacgcct gccggatcgt acaggcccgc attgtcgtaa
     5581 ccgcgggttt tcagctccgc cacgcgctcg ctcgccagcg ttttcatcga ctctttcgta
     5641 ccgaagtgaa tcgcgcccgt tgggcaggtc ttcacgcagg ctggttcttg cccaaccacc
     5701 acgcggtcaa cgcacagcgt acatttgtag acgcggttgt cttccgggtt gaggcgcgga
     5761 atgtcgaacg gacagcccgc aatgcaataa ccgcagccaa tgcactgctc ggactggaag
     5821 tcgacgatac cgttggcata ctgaataatt gcaccttccg ccgggcacgc tttcagacag
     5881 cctggatcgg aacagtgcat acagccgtct ttgcggatca gccattccag tttgtcgttc
     5941 tgctccactt ccgagaagcg catcaccgtc cacgatttgg cgcttaaatc attggggttg
     6001 tcgtacaccc caatgttatt gccgacggta tcgcgaatgt cgttccactc tgaacacgcc
     6061 acctgacagg ctttacagcc gatacaggtg gtgacgtcga tgagtttcgc cacttcttcc
     6121 tggaagtccc gcgcctgagg cgcgggggtc agaccgttag tcgcggaacg acgaatgata
     6181 tcttgagatt gataagccat atgtcgtctc cgttacacct tttccacatt cacaaggaag
     6241 gacttaaact ccggcgtctg cgtgttcgca tcaccgacga atggcgtcaa cgtattggca
     6301 ataaagcctt ttttcgcaac accttcatag ccccagtgaa taggaatacc gatggtatcg
     6361 atatctttgc cgttcgcttt cagcgtgcga atacgtttgg tcaccaccgc tttggctttg
     6421 atatagccac ggttggagga gactttcacg gtatcgccct gggcaatgcc aagtttattc
     6481 gccagcgact ccccgatttc cacaaactgc tctggttgca aaatcgcgtt caacagcgcg
     6541 tgtttggtcc agtagtggaa gtgctcggtc agacgatagg tggttccgac atacgggaac
     6601 ttatcggctt tacccaatgc ttcggcgtcg tctttaaaga tacgtgcagc cgggttcgag
     6661 ataacgtttg gatgcagcgg gttagttccc agcggcgttt caaacggctc gtagtgttcc
     6721 gggaacggac cttccgccat cttatcgagg gcaaacagac gccccatgcc ttcctgctgc
     6781 atgataaacg gcccgacgcc gctacccgga ggcgctgcgc tgtagtccgg aatatcccag
     6841 ccggtccact tagtgccgtc ccatttcagc aactgacgct tcggatccca cgggttaccc
     6901 tgcggatctg cggaggcgcg gttatacaga atgcggcggt taagcggcca tgcccatgcc
     6961 caacccagcg tgttaccgag gccagacgga tcggcgttat cacggcgtgc catctggttg
     7021 ccttccggcg tccagctacc ggcgaaaatc cagcaaccac aggaagttgt accgtcatcg
     7081 cgcagttggg caaacgagct aagttgttgg cctttcttga cgataaccgc accggttgcc
     7141 ggatcggtaa tatcggccag cgccttaccg ttgctctcca tcgctacttc ttccgacttc
     7201 ggctcatgcg gaatggcgta gttccaggtc atgttcagca cctggtccgg gttcgcgcca
     7261 ccctgttcgg catacatctt gcgcaagcgc aggaagatac cggagaggat ctcgccatca
     7321 gtcagcgcaa tccccggggc gtccgcacct ttccagtgcc actgcaacca gcgacctgag
     7381 ttaacgattg aaccgttctc ttctgcgaag caggtcgatg gcagacggaa cacttcggtc
     7441 tggatcttcg acgagtcaac ttcgttcagc tcaccgtggt tctgccagaa gttagaggtt
     7501 tcagtgttca gcgggtcgat ggtgacgagg aacttcagtt tcgacagaca actgatcact
     7561 ttgtttttgt tcgggaatga ggcaacaggg ttaaagccct ggcagatata gccattgacc
     7621 ttgccttctt tcatcatctc gaagtattgc aggacgtcgt agcctttatc ccacttcggc
     7681 aaccagtcaa agccccagct attttctgcc gtcgctttat caccaaagaa ggccttcatc
     7741 atagagacga agaatttcgg gtagttgccc cagtagtttc cctggccttc cagcagcggt
     7801 tttggcgtgt tggcggtaag gtaggtttgc agatcggtct gcttctcgct tggcagcgtc
     7861 atgtaacctg gcaggctctg cgacagcagc cccaggtccg tcagcccctg aatattggag
     7921 tgaccgcgca gggcgttaac gccgccgcca gccatcccca tgttaccgag cagcagctgg
     7981 atcatcgcca tcgtacgaat gttttgcgca ccaacggagt gttgcgtcca gccgagggca
     8041 tacaggaacg aggcagtttt atcgtgagca ctggtttctg cgatgtattc gcagactttc
     8101 aggaacgcgt cttttggcgt accacagatg ttttcaacca catctggcgt gtagcgggaa
     8161 acgtgctgtt tcagcaagtt ccacacgcag cgcggatgtt gcagcgtggt atcgcgtttg
     8221 gcgaagccgt tttcgtccag ttcataagtc caggaggatt tatcgtactt gcgtttttcc
     8281 gcgtcgtagc cggtgaacag gccatcttca aagccgtaat cctcacgcac gatcaggctg
     8341 gcgttggtat aggcttcagt gtattcgcgg ttgaattttt cattgttcag caggtacagc
     8401 aatacgcctg acaggaaagc aatgtcagta ccggaacgaa taggggcata gtagtcagcc
     8461 accgcagccg tacgcgtaaa gcgaggatcg atcacaatca gcttcgcgcc attgtgaatt
     8521 ttggcttcca tcgcccagcg gaacccgacc gggtgagctt cagcggcgtt accgcccatc
     8581 accacgacga ggttggcgtt cttgatgtcg acccagtggt tggtcatcgc accgcgacca
     8641 aatgttggag caagacttgc taccgttggt ccgtgtcaga cacgcgcctg gttgtcgacc
     8701 gcgagcatac ccagcgcgcg ggagaatttt tgcgttaaat agccggtttc gttactcgac
     8761 gcggaagcac acagcatccc ggtggagagc cagcggttaa cagtcacgcc ttcggcgttt
     8821 tgcgcaatgt agttagcatc gcggtcttct ttcatcagtt tggcgatgcg atcaaacgcc
     8881 tcttcccaac tgatttgctg ccatttatca gaacctggcg cacggtattc cggaaacttc
     8941 agacggcttt cggagtggat gaaatccacc aggccagcgc ctttcggaca aagtgcaccg
     9001 cggttgaccg ggtgatccgg atcgccttcg atatggaaga tagatgcttt ggcgtttttt
     9061 gctccgtcac cgaggctgta catcaacagc ccacagccta cggaacaata ggtgcaggta
     9121 ttacgggttt cgcgggtgcg cagcagttta tactgccggg tttccgcgag tgctacgctg
     9181 ggtgcaaaac ccagtgccgc tgccgtggtg cctgccatac cgccagcgca gatcttaaag
     9241 aactgccttc tgctgacctg catggattgc tccttgtttc gacattgtca cgtcccattt
     9301 acattcgctt gctgcgtgtg cagggagtgg gagttatttt tctttgcgga aggggccgca
     9361 aaggtccaga attggctcaa tttccctcca tccaggaagg gtttgtaaca gaataccata
     9421 atgttggtgt gtgtgttctt atctggttaa gagaaagtga aaaaaacaca gcaaaaagaa
     9481 atcgaaaatg tgacaaatat cacaggtgtt cgtcaaattg agttatggcg gcgtgatgat
     9541 ttacaacacc cacggttaga tgaggtcgcg gaagaagttc ccgttgcgct ggtctacaac
     9601 ggcatttcgc atgtggtgat gatggcgtcg cccaaagacc ttgagtactt tgcgctcggt
     9661 ttttcgcttt ccgaggggat aatagaaagt ccgcgcgata tcttcggcat ggatgtcgtt
     9721 ccttcctgta atggtcttga agtacaaatt gagctttcca gccgccgctt tatggggttg
     9781 aaggagcgcc gccgggcgct ggcgggacgt acgggctgtg gcgtatgcgg cgtggagcaa
     9841 cttaatgaca tcggaaaacc ggtgcagccg ctaccgttca cccagacgtt tgatctcaac
     9901 aaactggatg atgcattacg tcatctcaac gattttcagc cagtgggaca actgaccggt
     9961 tgtactcacg ccgccgcctg gatgttgcca tctggcgaac tggtcggcgg gcatgaagac
    10021 gtgggtcgcc atgtggcgct ggacaaactg ttaggccgcc ggtcacaaga aggggaaagc
    10081 tggcagcaag gtgcggtact ggtttccagt cgtgccagtt atgaaatggt gcaaaagtcg
    10141 gcgatgtgcg gcgtagagat tttgtttgcg gtgtctgccg cgaccacgct tgctgtagaa
    10201 gtggctgagc gctgtaatct gacactggta ggtttttgta aaccgggtcg ggcaacggtt
    10261 tatacccatc cgcagcgttt gagcaattaa ttaaaataag tcctgcgata tatattaaat
    10321 tgcaggactt attcatttcg tgaattttat tattttattt ataaacaaaa tgcgtgtagt
    10381 ggcgttttct
//
LOCUS       DAZFAB010000137        10363 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZFAB010000137 DAZFAB010000000
VERSION     DAZFAB010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10363
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.137"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            109..684
                     /gene="ssuE"
                     /locus_tag="ACHJWA_004182"
     CDS             109..684
                     /gene="ssuE"
                     /locus_tag="ACHJWA_004182"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFO4310668.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAA
                     EDLLYARFDSPALHTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLHSLRGNAHA"
     gene            677..1636
                     /gene="ssuA"
                     /locus_tag="ACHJWA_004183"
     CDS             677..1636
                     /gene="ssuA"
                     /locus_tag="ACHJWA_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFO4310669.1"
                     /translation="MRNIIKLALVGLLSVSTIVVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLLYVG
                     VEPPKPKAEVILVAENSPIKTAADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1633..2778
                     /gene="ssuD"
                     /locus_tag="ACHJWA_004184"
     CDS             1633..2778
                     /gene="ssuD"
                     /locus_tag="ACHJWA_004184"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFO4310670.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVDFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVENDFIPRRVAQS"
     gene            2790..3581
                     /gene="ssuC"
                     /locus_tag="ACHJWA_004185"
                     /pseudo
     CDS             2790..3581
                     /gene="ssuC"
                     /locus_tag="ACHJWA_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309044.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
     gene            3578..4345
                     /gene="ssuB"
                     /locus_tag="ACHJWA_004186"
     CDS             3578..4345
                     /gene="ssuB"
                     /locus_tag="ACHJWA_004186"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO4310671.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQEHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMQRGESETRLRKQG"
     gene            complement(4388..7000)
                     /gene="pepN"
                     /locus_tag="ACHJWA_004187"
     CDS             complement(4388..7000)
                     /gene="pepN"
                     /locus_tag="ACHJWA_004187"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFO4310672.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLCLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7266..8468
                     /gene="pncB"
                     /locus_tag="ACHJWA_004188"
     CDS             7266..8468
                     /gene="pncB"
                     /locus_tag="ACHJWA_004188"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFO4310673.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQVQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLEIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8637..10037
                     /gene="asnS"
                     /locus_tag="ACHJWA_004189"
     CDS             8637..10037
                     /gene="asnS"
                     /locus_tag="ACHJWA_004189"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFO4310674.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQTSKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTEAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2340 a         2643 c         2796 g         2584 t
ORIGIN      
        1 aaaaagatac acaaaattta tatttggcat tttcttgtct ctccgacaga ctgagcgaat
       61 tgttatcaat gttaacaaaa caagaacaat tggttataag gagagagtat gcgtgtcatc
      121 accctggcgg gtagtcctcg ctttccttct cgctccagct ccttgctgga atatgcgcgg
      181 gaaaaactaa atggcctgga tgttgaggtt tatcactgga atctgcaaaa cttcgccgcg
      241 gaagatctgc tttatgctcg tttcgatagt ccggcactcc ataccttcac cgaacagttg
      301 caacaggctg acgggctgat tgtcgccacg cctgtgtata aagccgctta ttccggtgcg
      361 ctgaaaaccc tgctcgacct gctgccagaa cgtgcgttgc aaggcaaagt ggtgctaccg
      421 ctggcgacgg gcggtacggt ggcccatctg ctggcggtcg attatgccct taagccagtt
      481 ttaagtgcgc tgaaagccca ggagatcctg cacggcgtgt ttgccgatga ctcacaagta
      541 attgattacc atcacaaacc ccagttcacg ccgaatttac aaacccgtct tgataccgcg
      601 ctagaaactt tctggcaggc attgcaccgc cgcgatgttc aggttcctga tcttcattct
      661 ctgcgaggta atgcccatgc gtaacatcat taaactggcg ctggtgggat tgcttagcgt
      721 ctctacgatt gtggttgctg cagaatcctc gcctgaagcg ttacgtatag gctatcagaa
      781 aggcagtatt ggtatggtat tggcaaaaag ccaccagtta ctggaaaaac gctatccgca
      841 atcaaaaatc tcgtgggtgg agttccccgc tggcccgcaa atgcttgagg cgttaaacgt
      901 tggcagtatt gatctcggca gtaccgggga tattccgcca atcttcgccc aggctgccgg
      961 ggctgatttg ctgtacgtgg gcgtcgagcc gccgaagcca aaagccgagg tgattctggt
     1021 ggcagaaaac agcccgatca aaaccgcagc cgatcttaaa ggccacaaag ttgcgttcca
     1081 gaaaggttcc agttcacaca atcttttact gcgcgcacta cgccaggccg gacttaagtt
     1141 tactgacatc cagcccactt atctgacgcc agctgatgcc cgcgccgcgt tccagcaagg
     1201 taacgttgac gcctgggcta tctgggatcc ctactactcc gctgcattat tacagggcgg
     1261 cgtgcgggtg ttgaaagacg gcaccgatct caatcaaacc ggatcgtttt atctggcagc
     1321 tcgcccctat gcagaaaaaa acggcgcttt tattcagggt gtactggcaa cctttagtga
     1381 ggccgatgcg ttaacccgca gccagcgcga gcaaagtatc gctttactgg caaaaacgat
     1441 gggcttaccg gcaccggtga ttgcctctta cttagatcat cgccctccta ccaccatcaa
     1501 accggttaac gccgaggttg ccgccttaca gcagcaaacg gcagatctgt tttatgaaaa
     1561 tcgtctggtg ccgaaaaaag tcgatattcg ccaacgcatc tggcagccca ctcaactgga
     1621 aggaaaacaa ttatgagtct gaatatgttc tggtttttac cgacccatgg tgacgggcat
     1681 tatctgggaa cggaagaagg ttcacgcccg gttgatcacg gttatctgca acaaattgcg
     1741 caagcggcgg atcgtcttgg ctataccggt gtgctgatcc caacggggcg ctcctgcgaa
     1801 gatgcgtggc tggttgccgc atcgatgatc ccggtgacgc agcggctgaa gtttcttgtc
     1861 gccctgcgtc ccagcgtaac ctcacctacc gttgccgccc gccaggccgc cacgcttgac
     1921 cgtctctcaa atggacgtgc gttgtttaac ctggtcacag gcagcgatcc acaagaactg
     1981 gcaggcgacg gcgtgttcct tgatcatagt gagcgctacg aagcctcggc ggaatttacc
     2041 caggtctggc ggcgtttatt gctgggggaa accgtcgatt tcaacggtaa acatattcat
     2101 gtgcggggag ccaaactgct cttcccgccg attcaacagc cgtatcctcc gctttacttt
     2161 ggcggatcgt cagacgtcgc ccaggagctg gcggcagaac aggttgatct ctacctcacc
     2221 tggggcgaac cgccggaact ggttaaagag aaaatcgaac aagtgcgggc gaaagctgcc
     2281 gcgcatggac gcaaaattcg tttcggcatt cgtctgcatg tgattgttcg tgaaactaac
     2341 gacgaagcgt ggcaggccgc cgagcggtta atctcgcatc ttgatgatga gactatcgcc
     2401 aaagcacaag ccgcattcgc ccgcacggat tccgtggggc aacagcgaat ggcagcgcta
     2461 cataacggca agcgcgacaa tctggagatc agccccaatt tatgggcggg cgttggctta
     2521 gtgcgcggcg gtgccgggac ggcgctggtg ggcgatggtc ctacggtcgc tgcgcgaatc
     2581 aacgaatatg ccgcgcttgg catcgacagc tttgtgcttt cgggctatcc gcatctggaa
     2641 gaagcgtatc gggtcggcga gttgctgttc ccacatctgg atgtcgccat tccggaaatt
     2701 ccccagccgc agccgctgaa tccgcaaggc gaagcggtgg agaatgattt tatccctcgc
     2761 agagtcgcgc aaagctaagg aagaagataa tggcaacgcc agtgaagaag tggttattgc
     2821 gcgttgcccc ctggttttta ccggtgggca tcgtgacggt gtggcaactg gcctcctcgg
     2881 ttggctggtt gtcgacgcgt attttgccct caccggaagg ggtggtgatg gcgttctgaa
     2941 cgctctcagc cagcggcgaa ttgtggcagc atctggcgat cagctcctgg cgggcgctga
     3001 ttggtttttc aattggcggc tcgctggggt tgattttggg actgattagc ggactgtcgc
     3061 gctggggaga aaggctgctg gatacctcaa ttcagatgtt gcgcaacgtg ccgcatctgg
     3121 cgctgatccc gctggtgatt ttgtggtttg gcattgatga atccgcaaaa atctttctgg
     3181 tggcgctcgg tacgctattc cccatttata tcaacacctg gcatggcatc cgtaatatcg
     3241 atcgcgggtt ggtggagatg gcgcgtagct atggattatc cggcataccg ctgtttatcc
     3301 atgtgatcct gcctggtgcc ctgccctcaa ttatggtcgg cgtgcgtttt gcgttgggcc
     3361 tgatgtggct gacgctgatt gttgccgaaa ccatttctgc caattcaggc attggttatc
     3421 tggcgatgaa tgcgcgggag tttttgcaaa cggacgtggt ggtagtcgct attattcttt
     3481 acgccctgct cggcaaactt gctgacgtca gcgcgcagct gttagagcgc ctctggttgc
     3541 gctggaaccc ggcttatcat ttgaaggagg ccacggtatg aatactgctc gtctgaacca
     3601 gggcacacca ttgttgctca atgcagtaag caaacattac gcggaaaata tcgtcctgaa
     3661 ccaactggat ttacatattc cggcaggtca gtttgtggcg gtggtgggcc gcagcggtgg
     3721 tggcaaaagt accctgctgc gcctgctggc aggtctggaa acgccaaccg ctggcgatgt
     3781 gttagcgggc accacaccgc tggctgaaat tcaggaagac acgcgaatga tgtttcagga
     3841 tgcgcgtctg ctaccgtgga aatcggtgat tgataacgtt gggttaggcc ttaaaggcca
     3901 gtggcgcgat gccgcgcgtc gggcgttggc tgcggtagga ctggagaatc gcgccgggga
     3961 atggcctgcc gcactttctg gcgggcagaa acagcgagtg gcgctggcaa gagcgttgat
     4021 tcatcgaccg ggtttattgt tgcttgatga accgctcggt gcgctggatg ccttaacgcg
     4081 actcgagatg caggatttga ttgtgtcact ttggcaggag cacggtttta ccgtactgtt
     4141 ggtgacgcat gatgtgagcg aagcggtagc aatggctgat cgggtgttgt taattgaaga
     4201 gggaaaaatt ggtctggatt tgacggtgga tattccccgt ccgcgccggt tagggtcggt
     4261 gcgattagcg gagctggaag cggaagtgtt gcaacgggtg atgcagcgtg gtgaatctga
     4321 aactcgcctg agaaaacagg ggtaaaatcc ccgaatggcg gcgctattgc cgccattcgg
     4381 ggatttatta agccagtgct ttggtgatct tctcgtacag atcgccagat agattttcca
     4441 gccctttcaa ctgttccagc gccgcgcgca ttttctcctg acgtttggca tcataacgtt
     4501 tcaggcgaat cagcggttca atcaggcgtg aagctacctg cgggttacgg ctgttcaggt
     4561 cggtaagcat ttccaccagg aactggtaac cgctcccatc ttcggcatgg aacgctgccg
     4621 ggttgctgcc cgcaaacgca ccaatcaacg aacgaatgcg gttcgggttg ctcatggtaa
     4681 atgagcgatg ctgcaacagg ccgcgcaccg tctccagcac attcgccgcc gggctggtgg
     4741 cttgcaggat aaaccattta tccatcacca gaccatcctg atgccacttg tcgtcgtact
     4801 cctgcatcag cgcgtcacgg caaggcagct gtgcagcaac cgccgcagaa agcgctgcca
     4861 gcgcatcggt catattgttt gcttcatggt attgcttgct caccagcacg tcagccagat
     4921 gcgtttcacc aaaagcaagg aagcgcaggc aggcattacg cagagtgcgt tttgcaatat
     4981 cttcatgctc aacacggtac tcgctctggt aattcgcgtt gtaaatagcc agtagctcat
     5041 ccgccagttc agtcgccaga gtacgagtga gtgcttcgcg tacttcggca atagcaatcg
     5101 ggtcgatgat atcgaacagt tcagccattt cattgacaga aggcagcgtc aggatttctg
     5161 ccgccagcgc cggatcaatc ttctcatcga gcagcaccgc gcggaaagcg tcagccacat
     5221 gtaccggcag agacagcggc tgcccttgct gatgacgggc gacgttcagc ttgatgtagg
     5281 ttgccagcaa actttgcgcc gcatcccaac gggaaaaatc attacgcgca tgacgcatca
     5341 ggaaggtcag ttgctgatcg ctccacttat attccagttt cactggcgca gagaattcgc
     5401 acagcagcgc aggcaccggc tggaagtaga cattatcaaa aacaaaggtc tgttccgcct
     5461 gggtgacgtt cagcacggaa ttcaccggat gaccgccttt ctgcaacggg atcactttgc
     5521 cttcgttgtc atacaattcg atggcaaacg gaatatgcag cggctgtttt tctgcttgat
     5581 ccggcgtggc tggcgtgcgc tggctgatgg tcagggtgta ctgctcggtt tccggattgt
     5641 agtcgtcttt gacggtcaca accggcgtac cggactggct gtaccaacgg cggaaatggg
     5701 agagatcgac attcgacgca tcttccatcg cctgaacgaa atcgtcgcag gtcgctgcac
     5761 tgccatcatg gcgctcgaaa tagagctgca tccctttctg gaagttttct tcgccaagca
     5821 gagtgtggat catacgaatc acttccgcgc ccttctcgta aacggtcagg gtgtagaaat
     5881 tgttcatctc aatgaccatg tccgggcgga ttgggtgcgc catcgggctg gcgtcttccg
     5941 caaactgcaa tccgcgcatg gtgcgcacat tattgatacg gtttaccgcg cgggaaccaa
     6001 ggtcagagct gaattcctga tcgcggaaga cggttaaacc ttctttcagg ctgagctgga
     6061 accagtcgcg gcaggtcact cggttaccgg tccagttatg gaaatattca tggccgataa
     6121 cgcgttcaat atcgaggtaa tctttgtcgg tggcggtatc ggtgcgggcc agcacatatt
     6181 tggagttaaa gatattcaga cccttattct ccattgcgcc catattgaag aaatccaccg
     6241 cgacgatcat atagatgtcg aggtcatact ccaggccaaa gcgttcttca tcccatttca
     6301 tggagttttt cagcgaggtc atcgcccacg gcgcgcgatc aaggttgccg cgatcgacgt
     6361 acagctccag tgctacttcg cgaccagaac gcgtggtaaa ggtatcgcgc agtacatcaa
     6421 agtcgcctgc caccagcgca aacaggtagc acggtttcgg gaacgggtcc tgccactgta
     6481 cccaatggcg tccgttttcc agttcgcctt gcgcaacgcg gttaccgttg gaaagcagga
     6541 agggatattt ggttttatcg gcaataattt tggtggtaaa acgcgccagc acgtccgggc
     6601 ggtcgagata atacgtaata tggcggaaac cttcggcttc acactgggtg caaagcgcat
     6661 cgcctgactg ataaagccct tccagcgccg tattcgccgc cgggcttatt tcattaatga
     6721 tcttaagcgt aaaacgctcc ggcaaattac tgatgaccag tgcgccctct tcttctttcc
     6781 aggcggtcca cggctcatca ttaatatgaa cagaaaccag tttgaggtct tcgccgttga
     6841 gacaaagcgg agcatctgat gcaccatgac ggacagcctg gctgaccgcg gtaacgaccg
     6901 tcttttgcgc gtcgaggtca aaggtcaagt caatatcagt aatctggtaa tccggcgcac
     6961 gataatcgtg acggtatttg gcttgtggct gttgagtcat aaataacctt tagcatcttt
     7021 tatagagtct ggtgttcagt ctattcctgt tgcgtaaatc gcgctatgca gaatcttcat
     7081 cttttcaggt acaaacgcct ttattgctac atttttataa catacaccgc gtaatgccat
     7141 cgaccagaaa ggtggcatat ggtgtgatcg gggttcaata aattgcgaaa caaggtatac
     7201 tccagcagtt cctgaagatg tttattgtac taaacgctcc tgtacgagga cgctactgcg
     7261 cacctatgac acaattcgct tctcctgttc tgcactcgtt gctggataca gatgcttata
     7321 agttgcatat gcagcaagcc gtgtttcatc attattacga tgtgcatgtc gcggcggagt
     7381 ttcgttgccg aggtgacgat ctgctgggta tttatgccga tgctattcgt gaacaggttc
     7441 aggcgatgca gcacctgcgc ctgcaggatg atgaatatca gtggctttct gccctgcctt
     7501 tctttaaggc cgactatctt aactggttac gcgagttccg ctttaacccg gaacaagtca
     7561 ccgtgtccaa cgataatggc aagctggata ttcgtttaag cggcccgtgg cgtgaagtca
     7621 tcctctggga ggttcctttg ctggcggtta tcagtgaaat ggtacatcgc tatcgctcac
     7681 cgcaggccga cgttgcgcaa gccctcgaca cgctggaaag caaattagtc gacttctcgg
     7741 cgttaaccgc tggtcttgat atgtcgcgct tccatctgat ggattttggc acccgtcgcc
     7801 gtttttctcg cgaagtacaa gaaaccatcg ttaagcgtct gcaacaggaa tcctggttcg
     7861 tgggcaccag caactacgat ctggcgcgtc ggctttccct cacgccgatg ggaacacagg
     7921 cacacgaatg gttccaggca catcagcaaa tcagcccgga tctagccaac agccagcgag
     7981 ctgcacttgc tgcctggctg gaagagtatc ccgaccaact tggcattgca ttaaccgact
     8041 gcatcactat ggatgctttc ctgcgtgatt tcggtgtcga gttcgctagt cggtatcagg
     8101 gcctgcgtca tgactctggc gacccggttg aatggggtga aaaagccatt gcacattatg
     8161 aaaagctgga aattgatcca cagagtaaaa cgctggtttt ctctgacaat ctggatttac
     8221 gcaaagcggt tgagctatac cgccacttct cttcccgcgt gcaattaagt tttggtattg
     8281 ggactcgcct gacctgcgat atcccccagg taaaacccct gaatattgtg attaagttgg
     8341 tagagtgtaa cggtaaaccg gtggcgaaac tttctgacag ccctggcaaa actatctgcc
     8401 atgataaagc gtttgttcgg gcgctgcgca aagcgttcga ccttccgcat attaaaaaag
     8461 ccagttaata tcatcaggga gctaatcggc tccctttttt tacctttaat tccgaaatct
     8521 ttcgctgcat ttgcgaattc tgcttgtctg attgcagatg ccaggtaaca taggtatccc
     8581 cccatttacg ggtggacaag tgtttatttt ttccgactat taacagagag aatattatga
     8641 gcgttgtgcc tgtagccgac gtactccagg gccgcgtagc cgttgacagc gaagtcaccg
     8701 tacgcggatg ggtacgtacc cgccgagatt caaaagctgg catctccttc ctcgccgttt
     8761 atgacggttc ctgctttgat cctgtacagg ctgtcatcaa taattctctg cccaattaca
     8821 atgaagacgt cctgcgtctg accaccggct gctcggtcat tgtgacgggt aaagtcgtgg
     8881 cgtcgccggg ccaggggcaa caatttgaaa ttcagaccag caaggttgaa gttgctggtt
     8941 gggttgaaga tccagacact tacccgatgg cggcaaaacg ccacagcatt gagtatctgc
     9001 gtgaagtcgc tcacctgcgt ccgcgcacaa acctgattgg tgccgtcgcg cgcgttcgcc
     9061 atacgctggc gcaggcgctg catcgcttct ttaacgagca gggattcttc tgggtttcaa
     9121 cgccactgat tactgcgtct gataccgaag gggctggcga aatgttccgc gtttctacgc
     9181 tggatctgga aaatctgccg cgtaacgatc agggcaaagt ggatttcgac aaagacttct
     9241 ttggtaaaga gtctttcctg accgtatctg gccagttgaa cggcgaaacc tacgcttgcg
     9301 cattatccaa aatttatacc ttcggcccga ctttccgtgc tgaaaactcc aacaccagcc
     9361 gtcacctggc ggaattctgg atgctggagc cggaagtggc gtttgctaac ctgaacgata
     9421 tcgcgggtct ggctgaagcc atgctgaaat atgtcttcaa agcggttctc gaagaacgcg
     9481 ctgacgacat gaaattcttc gctgaacgcg tagataaaga tgccgtttca cgtctggaac
     9541 gctttattga ggccgatttc gcgcaggtgg attacaccga agcagtaacc attctcgaaa
     9601 actgcggcag gaagtttgaa aacccggttt actggggcgt cgatctctct tctgagcatg
     9661 agcgttatct ggcggaagaa cactttaaag caccggtagt ggttaaaaac tatccgaaag
     9721 atattaaagc gttctatatg cgcctcaacg aagacggtaa aaccgttgcg gctatggatg
     9781 ttctggctcc gggcatcggt gagatcattg gtggctccca gcgtgaagag cgtctggacg
     9841 tgctggacga gcgtatgctg gaaatgggcc tgaacaaaga agattactgg tggtatcgcg
     9901 atctgcgtcg ctacggtact gttccgcatt ccggtttcgg tcttggtttt gaacgcctga
     9961 ttgcttacgt aactggtgtg caaaacgtgc gtgatgtgat tccgttccca cgaactccgc
    10021 gtaacgccag cttctaattt agcgtctaca agagccagca cccgctggct cttttttatc
    10081 ctttctgtca agttatctgt ttgttaagtc aatcaatcta tttgcaaccc cgccataacg
    10141 atcatcctgt tacggaatat tacattgcaa catttacgca caaaaaataa cccgcattct
    10201 tattgcggat tagttttttc ttagctgata gcacaatttt catactattt ttttggcatt
    10261 ctggatgtct gaaagaggat tttgggccag gtcgataaag tttccatcag aaacaaaatt
    10321 tccgtttagt taatttaaat ataaggaaat catataaata gat
//
LOCUS       DAZFAB010000138        10061 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZFAB010000138 DAZFAB010000000
VERSION     DAZFAB010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10061
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.138"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            6..81
                     /locus_tag="ACHJWA_004190"
     tRNA            6..81
                     /locus_tag="ACHJWA_004190"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="ACHJWA_004191"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="ACHJWA_004191"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(467..1225)
                     /gene="yhdZ"
                     /locus_tag="ACHJWA_004192"
     CDS             complement(467..1225)
                     /gene="yhdZ"
                     /locus_tag="ACHJWA_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFO4310675.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1233..2336)
                     /gene="yhdY"
                     /locus_tag="ACHJWA_004193"
     CDS             complement(1233..2336)
                     /gene="yhdY"
                     /locus_tag="ACHJWA_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO4310676.1"
                     /translation="MTKVLLSHPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFALERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2346..3527)
                     /gene="yhdX"
                     /locus_tag="ACHJWA_004194"
     CDS             complement(2346..3527)
                     /gene="yhdX"
                     /locus_tag="ACHJWA_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO4310677.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLNNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(3595..4620)
                     /locus_tag="ACHJWA_004195"
     CDS             complement(3595..4620)
                     /locus_tag="ACHJWA_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO4310678.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            complement(5051..5272)
                     /locus_tag="ACHJWA_004196"
     CDS             complement(5051..5272)
                     /locus_tag="ACHJWA_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310679.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            complement(5525..8674)
                     /gene="acrF"
                     /locus_tag="ACHJWA_004197"
                     /pseudo
     CDS             complement(5525..8674)
                     /gene="acrF"
                     /locus_tag="ACHJWA_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
     gene            complement(8686..9843)
                     /gene="acrE"
                     /locus_tag="ACHJWA_004198"
     CDS             complement(8686..9843)
                     /gene="acrE"
                     /locus_tag="ACHJWA_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFO4310680.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEVR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
BASE COUNT         2676 a         2593 c         2430 g         2362 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggactccc catgcgagag tagggaactg ccagacatca
      241 aataaaacaa aaggctcagt cggaagactg ggccttttgt tttatctgtt gtttgtcggt
      301 gaacactctc ccaaccagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg
      361 gcccggaggg tggcgggcag gacgcccgcc ataaactgcc agacatcaaa tcaagcgaaa
      421 ggccatccga aaggatggcc tttatgcttt tcgaactaac attcaattaa tggattacct
      481 gcgataaaaa tgccctcgta cgctctgact taggatgcgc aaaaaactca tccggtgcag
      541 cttgctccac tatttcgcca cgatccataa aaatcacccg gtcagcgacg gttcgcgcaa
      601 accccatctc atgggttaca cacaacattg tcatacccga ttgcgccagc ccaatcatcg
      661 tatccagcac ctctttcacc atctccggat cgagcgccga tgtgggctca tcaaacaaca
      721 taattttcgg cttcatacac agcgagcgcg caatggcaac gcgttgctgc tgaccacctg
      781 aaatctgtcc gggaaactta tgcgcatgtt cggcaattct cacccgctca aggtaatgca
      841 tcgccagagc ttcagcctct ttctttggca tcttgcgtac ccaaatcggt gccagggtac
      901 agttctgtag aacggtcaga tgagggaaga gattgaaatg ctgaaagacc attcccactt
      961 cctgcctgac gcgctcaata ttgcggatat cttcattaag ttcgatgcca tctaccacga
     1021 tccgtccctg ttgatgttct tccagatgat taatacaacg aatggttgtc gatttaccgg
     1081 aacctgaagg gccacacaga acgatccgtt ctcccggttg cacggttaaa tttatatttt
     1141 tcaaaacatg gaattgtcca taccatttat tgacgttttc cagcgtaatc atcgcgttag
     1201 caggttgcag taaaatttgg ctcatagtgt cctcaatgcg gtgtacgccc ggtgttaaaa
     1261 cgtttttcca gatgctggct atagcgcgac atgctgaaac agaagatcca gtagatcagc
     1321 gcggcgaaaa catacccttc cgtcgacata cccaaccagg cgggatcaac ggttgcttgc
     1381 tgaacgctac tgaaaagatc gaacaacccg ataatgatca ccaggctggt atctttgaag
     1441 agtgcgatga tggtatttac cagcccagga attaccagct tcaacgcctg tggcagaata
     1501 accagcccct gagttttcca gtaacccaac gccagcgact ctgccgcttc atattgccct
     1561 ttaggcagcg cctgtaatcc acctcgcacg acttccgcaa catatgctga ctgaaacagg
     1621 atcacgccaa ccagcgcgcg gatcagtttg tcgatactgg tgccttctgc cataaacaaa
     1681 ggcagcatga ccgaagacat aaacagaacg gtaatcagcg gcacgccgcg ccagaattcg
     1741 ataaaaatga ccgataagat gcgcacaatc ggcatatggg agcggcgacc taacgccagt
     1801 aagatccccc acggtagcgc ccccgcaatc ccaactgatg caataattaa agttagcgtc
     1861 agcccgcccc attgccgggt ttcaacccgc tcaagagcaa aaaaaccgcc atacatcagc
     1921 caccagacaa tcagtgggta aatcactgcc cagaccgcaa tatagcgacc gcgatgcggg
     1981 agtattttcc agaacattgg tgcgattgac acaaggccaa tcagtaatgc caagttaatt
     2041 cgccagcgtt ggtcgtgtgg gtaaagtcca tacataaact gaccaaatcg ttcgtggatg
     2101 aagacccaac aggcaccggc ttttgtgcag tcggcacgcg ttgagccaac ccagttagcc
     2161 tgcaaaaatg cccagttcag caacggtggg atcagttccc acatcaacca tatgcagcca
     2221 atagtcagca ggctattgct ccagctggag aacagatttt ttcgcaccca caccatcgcg
     2281 cggcttgagt tatggctcgc cgggcgcgag ggatgagaca gcaatacttt tgtcatcatg
     2341 actccttagc gttccacgat cgcgatgcgg cggttataga tattcatcag cagcgagata
     2401 gtcaggctga taatcagata gaccgacatg gtcatggcta tcgtctcgat ggcttgcccc
     2461 gtctggttca gcacggtgcc agcaaacagc gaaaccatat cgggatagcc aatagcggcg
     2521 gcaagagagg agtttttgac gatgttgaga tactggctgg ttaacggtgg aataatcacc
     2581 cgcagtgcct ggggaataat gacctggcgt agcgtaaccg ggttgggtaa tcccagtgac
     2641 cgagccgctt catgttgacc ataaggcact gcctggatcc ccgcgcggat aatctcggcg
     2701 ataaatgcag aggtataaac cgaaagtgcc agcgttaagg ctgccagttc aggaattaaa
     2761 accatcccgc cgcggaagtt aaagccttgt agggctggga catcccagtg tagtgctgcg
     2821 ccaaaaagcc attgcgccag taaaggcaaa ccgatgatca acactgcggc gatgggccag
     2881 gtgcggcgca gctgtccggt ctttatctgg tatgttttat taaagcggaa tagcccaaca
     2941 gaaaggacta tagccataac aacagccagg ataaacgcaa taaatccgtc tcccagctgc
     3001 ggtgacggaa tataaagccc acgattgctc aaaaaggcca gatcaaacgc gctcacagcc
     3061 tggcgtggtc cgggcaaatt gcgcaacacg gcaaagtacc agaagaagat ttgcagcagc
     3121 gggggaatat tacggaagat ctcaatataa attgtggaaa gctttcgtag cagccagtta
     3181 tccgaaagtc tcgccagacc gataaagaag cccaaaacag aagcgaacac aatacacaat
     3241 gcagaaacca gtagcgtatt gagtaagccg acaataaaaa cgcgtccgta ggtgtcgccc
     3301 tgctggtaat cgatcaaatg ctggacaata ccgaagccag cgccgcgatc cagaaaggca
     3361 aaacctgaag taatgccacg attattgaga ttcgttacag tgttgtgaaa taaccaacca
     3421 acaatgccga caacagcaac aacggcaagg atctggaata accaggcgcg aaccgtaggg
     3481 ttggcaaaag agagtgagcc tttaacggtt gagcggcgat gagacataga aacctcggaa
     3541 agcataacgc tgaagagagc gccgccgtag cgacgctcgt tacctcacac cgacttaacg
     3601 cacgggcggt gcgtactgaa taccgccgtt attccagaga ttattttgcc cacgtttaat
     3661 tttcagcggg ctttctgaac ctacgttacg ctcgaaaatt tccgagtagt tacccacctg
     3721 tttgatgatg ttataggccc atttattatc caacttcaga tccttgccgt aatcgccttc
     3781 tttacccagc agatgtgcca tatcaggcgt tgctggatta gccgcttttt catcgacgtt
     3841 ctgggaattg atgcccatct cttcagcatt tagcatggcg aaaagcgtcc agcgtacaat
     3901 cgagaaccat tcatcatcgc cacgacgaac taccggccca agcggttctt tagagataac
     3961 ctccggtaag acaatccatt cagccgggtt gcttaatttg atgcgcaggg catacagttg
     4021 ggattgatcc gaagccagcg tatcgcagcg accggattcc agtgccttcg ctgattcgtc
     4081 agaacgatcg aaagtcactg gtgtgtactt catattgttt gccttgaaat agtcggcgac
     4141 gttgagttcg gtatcagtac ccgcctgaat acagacggta gcgccatcca gttctttcgc
     4201 gctttttagc cccgctttat cgtgcgtcag gaagccaatg ccgtcgtaat aagtgacgcc
     4261 agtaaatgcc attcccatcc cggcatcgcg agatgaagtc caggtcgtat tacgggagag
     4321 caaatccact tcccctgact gtaaagccgt gaagcgttct tttgctgtga gtggggtata
     4381 tttcactttc gtgtcgtcac caaatacagc agcggcaaca ccacgacaaa catcaacatc
     4441 aatacctgaa aacttaccgt ctgcatcggc ataagagaac ccaggtaatc catcactgat
     4501 cccgcattgc acaaaacctt ttttctgaac ggcatcaagc gtcgcgccag catgcgcctg
     4561 atttgcaacg gcaagcagca cgctggcggc agccagtgtg gctatcatca tctttttcat
     4621 aatgcttcct gtgaggcgaa aattatcgtt ataggacgtt tgggaaacgt cttttgcctg
     4681 tctgtggcca tcctcttata agcaaaagta gtgccagctt ttgcgcacga tttcgcgtcg
     4741 caataagctg agtgtagaca ggaaataata aaaacagcgc cccgcaatgg tgcgaaaatg
     4801 aaacatgcgc catattttgg cgcaaaacat ttacgcgtgt tttactaagc agaataatga
     4861 ttgttagcgt gagtatattt ctggtgggaa gtgcgaaagt taaattgatg cgttatttcg
     4921 aaagcagcga aataacacga tataacatgc taaatcagca gagaattagc cagcagaata
     4981 tagcaatatt ttatcttatg taaagatact gtatcgttaa acagagcgcg aaactccgcg
     5041 ccctgttcag ttatttcgac agagcgatga tccccatcgt caggccagca acagccgctg
     5101 ccccgccagc aatagcccca gccgtttccc agttatcctg agtcgtgcct ttcatacagg
     5161 tattggtgtg aaccagacga ccctgatcgt cataaaccgg aacgcagggc gaatcgtgag
     5221 cacagcccgc cagcaatgcg gtgagcaatg ccacaggaat taatcttttc atggtgttac
     5281 ctcagtaata aacgctaacg cttaataagt taactctctt attaacgagc tgaatgccat
     5341 tttatcaccg gaggttataa atcttgcgga atgaataatg acaaattatg tgaattgtaa
     5401 aaatagtgaa gggagcggga gctatagaaa gcttttaaag agtgagcaaa agaatatctt
     5461 attaattttt atgggtttca ctggaaaaca taaaggcacc cgaaagcgcc tttatgtttc
     5521 agatttatcc tttaaagcaa cggcggatca ccacaaagaa caccggtacg aagaagattg
     5581 ccagcaacgt tgcagagacc attcctccca ttaccccgat acccactgcg ttctgcgcgc
     5641 cactgccggc accgttactg atagccagcg gtaatacgcc gagaataaag gcgagagagg
     5701 tcatcaggat aggacgcaga cgcatacgta ctgccattag tgtcgcttca acaacacctt
     5761 taccctcttt ctccatgaga tctttagcga actcaacaat caaaatagcg tttttggccg
     5821 acaagccaat tgtcgttagc aagcctacca taaagtagac gtcatttttt tgattaaaga
     5881 gtgtcgccgc cagcagcacg ccgacaatcc ctaacggcac gaccaacata accgagacag
     5941 gaattgacca gctttcatag agtgcagcaa ggcacaggaa aacaaccaca aaggaaattg
     6001 ctaccagagc gggagcctgg tttcccgata agcgttcctg atacgacata cccgtccagt
     6061 cataaccaat gcccgcaggt aattttgacg ctaggttttc catcaacgcc atggcatcgc
     6121 cggaactggt tcctggcgcg gcttccccct gaatctccat tgacggcaga ccgttgtagc
     6181 gttccagtcg cggagagcca tacacccaat gtgaagtggt aaaggcagag aatggcacca
     6241 tttcgccgtt ggtgctgcgg acataaagtt tatcgacatc ttctggcagc atacggaatt
     6301 tggcatccgc ctgaacatac acctttttca cgcgaccacg gtcgatgaag tcgttaacgt
     6361 aagtcccacc caacgccgtt gaaatggtct gattgatgtc agaaagtgaa acacctaatg
     6421 cctgcgcctt ttcctggtca acttccagtt taaactgcgc ggtgtcttcc aggccattgg
     6481 gacgcacgct gactaagctg gcaggatgtt gcgccgccat accaagcaac tggttacggg
     6541 cctgggttag ggcatcgtga cccagcccag ccttatcaat taactcaaag tcgaaacccg
     6601 ttgccgtgcc cagttcaaca atggctggca tattgaatgg aatgacaaaa ccgtcgcgga
     6661 tcttgcccaa ctccattttg gcacgatgga ttaccgcttc cgcactgttt tcatcaccat
     6721 tacgctcttc ccacggtttc agactgacga aggccatacc tgtagattca attggtcaac
     6781 gcaacagtta tgtgaaaaca tggggttgcg gaggtgcctg gccgctgaag ctaaagccgt
     6841 taaccgtaaa gacactttca acgtttgctt tctcgttctt cagatagtaa tccgtcactt
     6901 gatccaacac tttttgcgtc cgctcttgcg tcgcgccagc gggtaactga atcatggtca
     6961 gaaagacacc ctgatcctct tcaggtaaga aggaagacgg aagacgtaaa aacaacacca
     7021 ccattcctgc aacaatcagc gcatagatca gtaaatatcg tcctgtggaa ccgaggattt
     7081 tgccgacgct gttggtgtag tggttaacgc tatgatcgaa ggtggtatta aaccagccga
     7141 agaaaccacc cttattttcg tgatgctcag ctgaggtggg tttgagcagt gttgcacata
     7201 acgcaggggt aagaatcaat gccaccagaa cagaaagcgc cattgccgaa acgatggtga
     7261 tagagaactg acgataaatt gccccagtag aaccgccgaa gaatgccatc ggaataaata
     7321 ccgctgacag caccatcgcg atacccacca gtgcgccctg tatttgcgac attgattttt
     7381 ccgtcgcttc ttttggcggg agcttatcct ccatcatcac gcgctcgacg ttctccacca
     7441 ccactatcgc atcatcgacg agcagcccga tggcaagcac catcccgaac atcgttagtg
     7501 tgttgatgga gtaaccaaaa gcggcgagga tggcaaacgt ccctaacaac accacgggta
     7561 ccgcaatggt ggggatcagc gttgctcgca tattctgcaa gaacagatac atcaccagga
     7621 acaccagcat aatagcttcg aacagtgttt ttaccacttc gtgaatagaa agctggacga
     7681 atggcgtggt gtcataaggg tagagaacct tcattccctg cgggaagaat ggctgtaatt
     7741 ccgccagttt tgccttaatg gctttcgcgg tatcgagagc attcgcgccg gttgccagct
     7801 taatacccag gcccgccgcc ggttttccgt tgatgcgagc gataacgtta tagttttcac
     7861 cgccaagttc aacccgtgcg acatctttca gacgcaccac cgagccgtca ctgtttacgc
     7921 gcagggtcac tttgccgaat tcttccggat tttttaaccg cgtctgagca ataatcgagg
     7981 cattcaactg ttgccctggt aacgctggcg ttccgcccaa ctgtccggca gcgatctgag
     8041 cgttctgtac cttcaactgg ttaatcacat caaccggtgt cagtttatat ttgtttagca
     8101 gatcggcgtc cagccagata cgcatcgcat actgtgcgcc gaaaagctgt acgtcaccga
     8161 cgctattcag acggctaagc gtatctttaa cgttagaggc cacatagtcc gagatatcgt
     8221 cctgtgtggt gtctgggtta tcagagacaa agcctgccac catcaaatag ctgctactgg
     8281 acttttcaac actgatcccc tgctgctgaa cctcctgcgg cagcaacggc gtggcgagct
     8341 ggagtttgtt ctgcacctgc acttgcgcga tatcaggatc ggtcccggac tggaaggtaa
     8401 gggtaattgt cacgctaccg gcggaatcgc tggtggagga catatacatc aggttatcga
     8461 taccgttcat attctgttcg ataacctgcg tcaccgtatc ctgcacggtc tgcgcatcag
     8521 cgcccggata gtttgctgaa acagaaaccg caggcggtgc aatggttgga tactgagcga
     8581 cgggcaattg taggatcgcc agtgcgcccg ccatcatcag aataatggcc agcacccatg
     8641 caaatatcgg tcgtcgaata aaaaagtttg ccatgtcaga ttaccttact tcgatgcagt
     8701 atctgcgggg gtatcggtag tggctttaac ctgctctccc ggacgcgctt tttgcaggcc
     8761 gctgacaatg acttgatcgc cagatttcag accttcgcta atcaaccatt tatcgccaat
     8821 cgcctgactg gcaacgacag ggcgcacttc cacctgactt ttatcgttaa caatcagcac
     8881 ggttgcatca ccacgcggtg tgcggctaac gccttgttgc gggatgagaa tggcgtcagg
     8941 ttggacgcct tcatcaatcc gtgcacgcac aaacataccc ggcaaaagcg tatgttgcgg
     9001 gttagggaag acagcacgta gggttatgga gccggtgctt tcatcaacgg tcacatcgga
     9061 gaattgcagc gtacctttca ggggataggt ttgaccgttt tccatgacca actctacgtt
     9121 gctggtggcg ttttccttat gcaaatttcc ttgctctacg gactgcttca gcctcataaa
     9181 atcgttgctg gattgggtca catcaacata gataggatcg agctgctgga cagttgccag
     9241 ttcagtcgtt tgcccattag tgacaagagc gccttcggtc acagtcgatt tgccgatacg
     9301 tccgctaatt ggcgcagtca ctttggtata agcaagattg atgcgagcgc tttcgactgt
     9361 ggcttttgcg gcaatcacgg cggcatcggc ctggcgagca tcagcaatgg cctggtcgta
     9421 ctcctgctgg ctgatgtatt tcgtacccac gagcggaacg taacgtttta ccgtcaaatg
     9481 cgcgatggcg gcggcggctt cacttttcgc cagttcgcct ttcgcgctgt cataatttgc
     9541 ctgataggtc gcgggatcga tctggtacag ggactggcct gcttgcacat cgctgccttc
     9601 agtgaaattg cgattcagta cgatcccgct aacctgtggg cgaacttcgg ctatacgata
     9661 agcattggtg cggcctggta attcagtctt aacttctaac ggggccgttt ttacaatatg
     9721 aacggtaacc tgcggttcac cgacgtgagc tttctcttct cccttatcgt tacaaccggc
     9781 gattaaagcc gcggagatca gaataaagga gggcaggagg aaaaacctgg catgtttcgt
     9841 cattactatt cctcaaaaaa ccaaaagcgc gttatttacc caaaaaggca acgcgttaac
     9901 tcgcagaaag aaaaatacag ttcgctatcc tacaaattat cattcgtcga tgtaaggaat
     9961 agttatgaat acaggcgtct caaggcacat aaacacaaaa aaagattaat attctactgt
    10021 tttattttga cgcgggttga aagaggcaga attaaaaaat c
//
LOCUS       DAZFAB010000139        10015 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZFAB010000139 DAZFAB010000000
VERSION     DAZFAB010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10015
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.139"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            114..188
                     /gene="tnaC"
                     /locus_tag="ACHJWA_004199"
     CDS             114..188
                     /gene="tnaC"
                     /locus_tag="ACHJWA_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFO4310681.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            409..1824
                     /gene="tnaA"
                     /locus_tag="ACHJWA_004200"
     CDS             409..1824
                     /gene="tnaA"
                     /locus_tag="ACHJWA_004200"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFO4310682.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENASNIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1915..3162
                     /gene="tnaB"
                     /locus_tag="ACHJWA_004201"
     CDS             1915..3162
                     /gene="tnaB"
                     /locus_tag="ACHJWA_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFO4310683.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFLVDYSILRDATSSTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3294..4469
                     /gene="mdtL"
                     /locus_tag="ACHJWA_004202"
     CDS             3294..4469
                     /gene="mdtL"
                     /locus_tag="ACHJWA_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFO4310684.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIITSVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WTMAIMGIAVLMLSLFILKETRPAAPAASDKSRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLTVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGISAWNMLIGILIACSIVSLLLIMFVAPGRPVTAHEEIHHHA"
     gene            4444..5403
                     /gene="yidZ"
                     /locus_tag="ACHJWA_004203"
     CDS             4444..5403
                     /gene="yidZ"
                     /locus_tag="ACHJWA_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFO4310685.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKQIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHEKWDLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRDTIKNLYASMA"
     gene            5560..6309
                     /gene="yieE"
                     /locus_tag="ACHJWA_004204"
     CDS             5560..6309
                     /gene="yieE"
                     /locus_tag="ACHJWA_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFO4310686.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWVSKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWELDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTMKAFSPN"
     gene            6331..6897
                     /gene="yieF"
                     /locus_tag="ACHJWA_004205"
                     /gene_synonym="chrR"
     CDS             6331..6897
                     /gene="yieF"
                     /locus_tag="ACHJWA_004205"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFO4310687.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(6951..8288)
                     /gene="adeP"
                     /locus_tag="ACHJWA_004206"
     CDS             complement(6951..8288)
                     /gene="adeP"
                     /locus_tag="ACHJWA_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFO4310688.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRIRDLSPCVIIVALLFILKIVFIDAH"
     gene            8454..9119
                     /gene="yieH"
                     /locus_tag="ACHJWA_004207"
     CDS             8454..9119
                     /gene="yieH"
                     /locus_tag="ACHJWA_004207"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFO4310689.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFREFGIHVDLKEIFT
                     RFKGVKLYEIIDIISKEQGVTMVQADAEHVYRAEVARLFDTELEDIAGANELLASINV
                     PMCVVSNGPVSKMQHSLGKLNMLHYFPEKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSSAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTELSQLPALWKARGWN
                     ITR"
     gene            complement(9256..>10015)
                     /gene="espY4"
                     /locus_tag="ACHJWA_004208"
     CDS             complement(9256..>10015)
                     /gene="espY4"
                     /locus_tag="ACHJWA_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector EspY4"
                     /protein_id="HFO4310690.1"
                     /translation="KYHFDNSRVEFFIFKDMQENAQNIINNMIESDRIKFVESIINKM
                     IPPPDGTILTENPKEYVQKQIKESHKESATNVTFDYKELAPIFEGVEEKQIKALSNQL
                     EHIKSFKTDYDSRLNKYARDFYYFSSAFAINWEDLLKNYQEIRASIKDYDDLLREIKE
                     LIISRNKSLEDKRTLFDNKRDNWNSIEVQDEVNALNAKIVDCDDKIRSKLTIVRNNRL
                     ENQYKDDKNISDAMRNILDWFERYPDIVQNITQA"
BASE COUNT         2502 a         2443 c         2405 g         2665 t
ORIGIN      
        1 ataaacacag ccaataacta aatgtgattc gattcacatt taaacaattt cataatagac
       61 aaatttcttg agtattatac tgtagcttcg tgtctttcga ggataagtgc attatgaata
      121 tcttacatat atgtgtgacc tcaaaatggt tcaatattga caacaagatt gtcgatcacc
      181 gcccttgatt tgcccttctg tagccatcac cagagccaaa ccgattagat tcaatgtgat
      241 ctatttgttt gctatatctt aaatttgcct tttgcaaagg ccatctctcg tttatttact
      301 tgtgttagta aatgatggtg cttgcatata tacatggcga attaatggat attgcagatg
      361 caatattcac agggatcact gtaattaaaa taaatgaagg attatgtaat ggaaaacttt
      421 aaacatctcc ctgaaccgtt ccgcattcgt gttattgagc cagtaaaacg taccactcgc
      481 gcttatcgtg aagaggcaat tattaaatcc ggtatgaacc cgttcctgct ggatagcgaa
      541 gatgtgttta tcgatttact gaccgacagc ggcaccgggg cggtgacgca gagtatgcag
      601 gccgcgatga tgcgcggcga cgaagcttac agcggcagcc gcagctacta tgcgttagcc
      661 gagtcagtga aaaatatctt tggttatcaa tacaccattc cgactcacca gggccgtggt
      721 gcagagcaaa tctatattcc agtactgatt aaaaagcgcg agcaggaaaa aggcctggat
      781 cgcagcaaaa tggtggcgtt ctctaactat ttctttgata ccacgcaggg ccatagccag
      841 attaacggct gtaccgtgcg taacgtctat atcaaagaag ccttcgatac gggcgtgcgt
      901 tacgacttta aaggcaactt tgaccttgag ggattagaac gcggtattga agaagttggt
      961 ccgaataacg tgccgtatat cgttgcaacc atcaccagta actccgcagg cggtcagccg
     1021 gtttcactgg caaacttaaa agcgatgtac agcatcgcga agaaatatga tattccggtg
     1081 gtaatggact ccgcgcgctt tgctgaaaac gcctatttca tcaagcagcg tgaagcagaa
     1141 tacaaagact ggaccatcga gcagatcacc cgcgaaacct acaaatatgc cgatatgctg
     1201 gcgatgtccg ccaagaaaga tgcgatggtg ccgatgggcg gcttgctgtg catgaaagac
     1261 gacagtttct ttgatgtgta caccgagtgc agaacccttt gcgtagttca ggaaggcttc
     1321 ccgacgtatg gcggcctgga aggcggcgcg atggagcgtc tggccgtagg tctgtatgac
     1381 ggcatgaatc tggactggct ggcttatcgt attgcgcagg tgcagtatct ggtcgatggt
     1441 ctggaagaga ttggcgttgt ctgccagcag gcgggcggtc acgcggcatt cgttgatgcc
     1501 ggtaaactgc ttccgcatat cccggcagat cagttcccgg cacaggcgct ggcctgcgag
     1561 ctgtataaag tcgccggtat ccgcgcggta gaaattggct ctttcctgtt aggccgcgat
     1621 ccgaaaaccg gtaaacaact gccatgcccg gctgaactgc tgcgtttaac cattccgcgc
     1681 gcaacatata ctcaaacaca tatggacttc attattgaag ccttcaaaca tgtgaaagag
     1741 aacgcgtcga atattaaagg gttaaccttt acctacgaac caaaagtatt gcgtcacttc
     1801 accgcaaaac tgaaagaagt ttaattaata ctactgagtg gctataagga tgttagccac
     1861 tctcttaccc tacatcctca ataacaaaaa tagccttcct ctaaaggtgg catcatgact
     1921 gatcaagctg aaaaaaagca ctctgcattt tggggtgtta tggttatagc aggtacagta
     1981 atcggtggag gtatgtttgc tttacctgtg gatcttgccg gtgcctggtt tttctggggt
     2041 gcctttatcc ttatcattgc ctggttttca atgcttcatt ccgggttatt gttattagaa
     2101 gcaaatttaa attatcccgt cggctccagt tttaacacca tcaccaaaga tttaatcggt
     2161 aacacctgga acattatcag cggtattacc gttgccttcg ttctctatat cctcacttat
     2221 gcctatatct ctgctaatgg tgcgatcatt agtgaaacga tatcaatgaa tttgggttat
     2281 cacgctaatc cacgtattgt cgggatctgc acagccattt tcgttgccag cgtattgtgg
     2341 ataagctcgt tagccgccag tcgtattacc tcattgttcc tcgggctgaa gattatctcc
     2401 tttgtgatcg tgtttggttc tttcttcttc ctggtcgatt actccattct gcgcgatgcc
     2461 accagctcca ctgcgggaac gtcttacttc ccgtatatct ttatggcttt gccggtgtgt
     2521 ctggcgtcat ttggtttcca cggcaatatt cccagcctga ttatttgcta tggcaaacgc
     2581 aaagataagc taatcaaaag cgtggtcttc ggttcgctgc tggcgctggt gatttatctc
     2641 ttctggctct attgcaccat ggggaatatt ccgcgagaaa gctttaaggc gattatttcc
     2701 tcaggcggca acgttgattc actggtgaaa tcgttcctcg gcaccaaaca gcacggcatt
     2761 atcgagtttt gcctgctggt gttctccaac ttagctgtcg ccagctcgtt ttttggtgtc
     2821 acgctgggat tgttcgatta tctggcggac ctgtttaaga ttgataactc ccacggcggg
     2881 cgtttcaaaa ccgtgctgtt aaccttcctg ccacccgcgt tgttgtatct gatcttcccg
     2941 aacggcttta tttacgggat cggcggtgcc ggactgtgcg ccactatttg ggcggtcatt
     3001 attcccgcag tgctggcaat caaagctcgc aaaaagtttc ccaatcagat gttcacggtc
     3061 tggggcggca atcttattcc ggcgattgtc attctctttg gtataaccgt aattttgtgc
     3121 tggttcggca acgtctttaa cgtgttacct aaatttggct aaatccttca agaagccagt
     3181 cattcgctgg cttcttgcct ctcaggaaat cacttatgtc caaatggcaa ctcgcctgat
     3241 cctccttcac cacgtatgct ttgcgtcacc ttactatcag gacgctttag cccatgtccc
     3301 gctttttgat ttgtagtttt gccctggttt tactttatcc cgctgggatt gatatgtacc
     3361 tcgttggttt accgcgcatc gccgccgatc tcaatgccag cgaagcgcag ttacatattg
     3421 cgttctccgt ttatctggca ggtatggcag ctgcgatgtt atttgccggt aaagtggccg
     3481 atcgttcagg gagaaaaccg gtcgccatcc ccggcgcggc gctatttatt atcacttcgg
     3541 tgttctgttc actggctgaa accagcacgt tatttcttgc agggcgcttt ctacaggggt
     3601 tgggcgcggg ctgttgttac gtggtggcat tcgccatttt gcgcgacacg ctggatgatc
     3661 gacgtcgggc aaaagtgctg tcgttactca acggcattac ctgcatcatt ccggtgttag
     3721 cgccggtgct cggtcatctg attatgctta aattcccgtg gcagagtctg ttctggacga
     3781 tggcaataat gggcatcgcg gtactgatgt tgtctttgtt tattttaaaa gaaacgcgcc
     3841 ctgccgcccc cgcagcttcg gacaaatcac gagaaaatag cgagtcgctg cttaatcggt
     3901 ttttcctcag ccgtgttgtt atcaccaccc tcagcgtttc ggtgatcctc accttcgtca
     3961 atacatcgcc ggtattgctg atggaaatca tgggttttga gcgcggagaa tacgccacca
     4021 ttatggcgtt gaccgctggc gtcagcatga ccgtttcatt ttccacgcca tttgcgctgg
     4081 gaatttttaa gccacgtacg ttgatgatca cctcacaggt gttattcctg gcggcgggca
     4141 tcactcttac cgtttcacct tcccatgcgg tttctctgtt tggtatcacg ctgatttgcg
     4201 ccggtttctc ggtaggtttt ggtgtggcga tgagtcaggc gttagggccg ttttcattac
     4261 gcgcgggcgt agccagctcg accttaggta ttgcgcaggt ttgcggttcg tcactgtgga
     4321 tttggctggc agcggtggtt ggtatcagcg catggaatat gctgatcggg attctgattg
     4381 cctgtagcat agtgagcctg ttgctgatta tgttcgtcgc gcctggacgc ccggttaccg
     4441 ctcatgaaga aatccatcac cacgcttgat ctcaatctgc tgctctgtct gcagctgctg
     4501 atgcaggagc gcagcgtaac caaagcggcg aagcggatga acgtgacacc ttcggcggtg
     4561 agtaagtcgc tggcaaagtt aagagcgtgg tttgacgacc cgctctttgt gaactcaccg
     4621 ttggggctgt cgcccacacc gctaatggtc agcatggagc aaaatctggc ggagtggatg
     4681 caaatgagca acctgctgct ggataaaccg caccaccaga caccgcgcgg cctgaagttt
     4741 gagctggcgg cagaatcacc gctaatgatg atcatgctta atgcgctgtc gaaacagatc
     4801 taccaacgtt acccgcaggc gaccatcaaa ttacgtaact gggattacga ttccttagat
     4861 gccattactc gtggtgaagt ggatatcggt ttttccggtc gcgaaagtca tccccgttcg
     4921 cgggagctgt taagctcgct accgttagcc attgattatg aagtgttgtt tagtgatgtg
     4981 ccctgcgtct ggttacgcaa agatcatccg gcactgcatg aaaagtggga tctcgatacc
     5041 tttttacgtt atccgcatat cagcatttgc tgggaacaga gcgatacctg ggcgctggac
     5101 aatgtgttac aggagctggg gcgcgaacgt acgattgcaa tgagcctgcc ggaattcgag
     5161 cagtcactgt ttatggcagc gcagcccgac aatctgctcc tggcgaccgc gccgcgctac
     5221 tgtcagtact acaatcaact ccaccaactg ccgttggttg ctcttcctct cccgtttgac
     5281 gaaagccagc aaaaaaagct ggaagtccct tttaccctgc tgtggcataa acggaacagc
     5341 cataatccga agatcgtctg gttacgggat accattaaaa atctttacgc gtcgatggca
     5401 taaccgaatc gtatgaaacg cgacccgatt tcacaataaa atgtaaaaaa gttgtaataa
     5461 gcttgtctga atcgaacttt tagccgcttt agtctgtcca tcattccagt aaatgattac
     5521 tcttgtattc ataatggacc attaagcatg gagcgaaaaa tggcgactca ctttgcccga
     5581 gggattttaa cggaaggaca tctgatttct gttcgtctcc cctcccagtg tcatcaagaa
     5641 gcccgaaaca ttccccctca tcgtcaaagc cgttttctgg cgtccagagg tttactcgca
     5701 gaactgatgt tcatgctgta tggcattggc gaattgccgg aaatcgtcac cctgccgaaa
     5761 ggtaaaccgg ttttcagtga taaaaatttg ccttcgtttt ctatttccta tgccgggaat
     5821 atggttggcg tggcgttaac aaccgaaggt gaatgtggcc tcgatatgga actgcagcgt
     5881 gcgacgcgcg ggtttcatag cccacacgcg cccgataacc acacattttc cagcaatgaa
     5941 tcgctatggg tcagtaaaca aaatgatcca aacgaagcgc gggcgcagct catcacgctg
     6001 cgccgaagcg tgctaaaact aaccggtgat gttttgaatg acgatccgcg cgatctgcaa
     6061 ctgctgccca ttgccggacg cctgaaatgt gctcatgtaa atcatgtaga agcgttatgc
     6121 gacgcggagg acgtgctggt gtggtccgtg gcggtcacgc ccacgattga aaagctcagt
     6181 gtctgggagt tagatggcaa acacggctgg aaaagcctgc cggatattca cagccgcgcc
     6241 aacaatccta ccagccggat gatgcgtttt gcccagctct ctaccatgaa ggctttttcg
     6301 ccaaattgat agacaaccac aggagtcatt atgtctgaaa aattgcaggt ggttacgtta
     6361 ctggggagcc tgcgcaaagg ctcatttaat ggcatggttg cacgtaccct accgaaaatt
     6421 gctccggcga gcatggaagt caatgcgtta ccttccattg ccgacattcc cctgtatgac
     6481 gctgacgtac agcaggaaga aggttttcca gcaacggttg aagctctggc ggaacagatc
     6541 cgtcaggctg acggtgtggt gatcgtcacg ccggaatata actattcggt accgggtggg
     6601 ctgaaaaatg ccatcgactg gctttcccgc ctgccggatc aaccgctggc cggtaaaccg
     6661 gtgttgattc agaccagctc aatgggcgtg attggcggcg cgcgctgtca gtatcacctg
     6721 cgccagattc tggttttcct cgatgcaatg gtgatgaaca agccggaatt tatgggcggc
     6781 gtgattcaga acaaagttga tccgcaaacc ggagaagtga ttgatcaggg tacgctggac
     6841 cacctgaccg ggcaattgac cgcatttggc gagtttattc agcgagttaa gatctaaata
     6901 aaaaacccgc cagcaatcat gcatggcggg tttttaacgc gctatcgatt ttagtgagcg
     6961 tcgataaata caatcttcag gataaacagc agcgcaacga tgattacgca cgggctaagg
     7021 tcacgaatac gcccggtacc aatcttcatc acgcagtagg agataaagcc cagcgcaata
     7081 ccttcggtaa tcgagaagct gaacggcatc atcacagcgg taataaacgc cggaacagat
     7141 tcagtaagat cctgccagtt cacgcgagca agacttgagg tcatcaacac gccaacgtaa
     7201 atcagcgcgc cagctgcagc gtagcctggc accattcccg ccagcggcga cagaaagata
     7261 accagcagga acagcagacc aacaaccact gccgtcagac cggtacgacc gccaaccgat
     7321 acgccggaag aggactcaat ataagccgta acggaagaag taccgataaa cgaaccggtc
     7381 acggaagaga tactgtcgac atacagcgcc tgcttcatgc gcgggaattt ccccttctca
     7441 tctgccagac cggctttatc ggtcacgcca atcaacgtgc cggaggagtc aaacaggttg
     7501 accagcatga aagagaaaat cacccctgcc agcccgaggt taaacgaccc ggctaaatct
     7561 acatgaccca cgacagtcat tacgctcggc ggcgcagaaa cgatgccatt gtagtggaca
     7621 tcacccagca tccagcccag cagcgttgtc accacgatag aaaccagcac cgctgcgtga
     7681 atgttgcgtg aggccagaat agcaatgatg aagaagccga ggatccccag cagcacgctg
     7741 tgagaagtca gattaccgat gctgaccagc gtttccgggt tagcgacaat cacacctgcg
     7801 tttttcagcc ccatcatgcc aatgaacaga ccgataccgc tggtaatgcc cacacgcaga
     7861 ctcaccggaa tgttggcaat catccagtag cgaacgcgga aaatcgtcag taacagcaga
     7921 ccaatcgcgc cccagaagat tgcgcccatc ccgacttgcc acggcaagcc catcgcctgc
     7981 acaacgacaa aagcgaagaa cgcattcagg cccatagcgg gtgccagtgc aactggcagg
     8041 ttagcaaaca gccccatcat aatactgccg aatgcagcaa tcagacaggt agtgacgaag
     8101 acggcgctgg tatccatgcc agcaacgcca agaatttgcg ggttaacaaa aacgatgtaa
     8161 accatcgtca ggaaggtggt aaaaccggcg atcacttcgg tccgtgccgt cgtgccatgt
     8221 tcgcgcagtt taaacacgcg ttccagcatc ccctggccag aagcctgggt tgtgtgttgt
     8281 tgactcatta tctatttccg aacaaggagg gaaaatccgt cgctatcgta taccaaaatg
     8341 agacaatatg gacggctgaa gataactttt ttcattgtat ttgcttatac ggcaacgatt
     8401 gcgtcatgcg aattggcatg acgaaaacgt taaactaacg ataaaggaaa gacatgtccc
     8461 agatagaagc ggtatttttc gactgcgacg gtacgctggt cgatagcgag gtcatttgct
     8521 ctcgcgccta tgtcacgatg ttcagggaat ttggcattca cgttgacctg aaagagattt
     8581 ttacccgttt taaaggcgta aaactctacg aaatcatcga tatcatcagt aaagaacagg
     8641 gcgtgacgat ggtccaggcc gatgccgagc acgtttaccg cgcagaagta gcacgtctgt
     8701 ttgataccga acttgaggac atcgcaggag cgaacgaatt gctggcgagc attaacgtgc
     8761 cgatgtgcgt cgtctctaac ggtccggtca gcaaaatgca gcattcgctt ggtaagctca
     8821 acatgctgca ctacttcccg gaaaaactgt tcagcggata tgacattcag cgttggaagc
     8881 ccgatccggc attaatgttc catgcggcaa aagcgatgaa tgtgaacgtt gaaaactgca
     8941 ttctggtcga tgactccagc gctggcgctc aatcagggat agatgccgga atggaagtgt
     9001 tttacttctg cgctgacccg cacaataagc cgatcgttca cccgaaagtc accaccttta
     9061 ccgagctgtc gcagctgcct gcgctctgga aggcacgcgg ctggaatata acgcgataat
     9121 cgcctcctta cttcaacact tacacatcag tcacgctggt ttgagataca gaacaccagg
     9181 tgtctgatga gtaagcgtct attccctatg aataatttat gtcgttattc gtcctgtcat
     9241 ccaccctctg tatggttaag cctgggttat attttgtaca atatcgggat accgttcgaa
     9301 ccaatccaga atattacgca tcgcgtcact gatgttttta tcgtctttat attgattctc
     9361 aagtctatta tttcgaacaa tagtaagctt agaacgtatt ttatcatcac aatcgacaat
     9421 tttcgcattc aatgcattta cttcatcctg cacttctata ctattccaat tgtccctttt
     9481 gttatcaaat aaagtccttt tatcctctaa tgatttatta cgacttatga ttaactcttt
     9541 aatttctcgc aataagtcat cgtaatcttt aatggaagct cttatttcct gatagttttt
     9601 tagtaaatcc tcccagttaa ttgcaaatgc actggaaaaa tagtaaaaat cgcgagcata
     9661 cttattgagc cttgagtcgt aatctgtctt aaacgatttt atatgttcca gttggtttga
     9721 tagtgcttta atttgttttt cttcaacacc ttcaaaaatg ggtgcaagtt ccttataatc
     9781 aaatgttaca tttgtagcag attctttatg agactccttt atttgctttt ggacatattc
     9841 ttttggattt tcagtaagta ttgttccatc tggcggcgga atcattttat ttattataga
     9901 ttcaacaaac ttaatccggt cactttctat catattgtta atgatatttt gtgcattttc
     9961 ctgcatatct ttaaaaataa aaaactcaac cctactatta tcaaaatgat atttt
//
LOCUS       DAZFAB010000140         9950 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZFAB010000140 DAZFAB010000000
VERSION     DAZFAB010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9950
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.140"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..198)
                     /locus_tag="ACHJWA_004209"
     CDS             complement(<1..198)
                     /locus_tag="ACHJWA_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310691.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWPCG
                     EFFSLNYKLTKRIYFITFIYNK"
     gene            276..1370
                     /gene="ddlA"
                     /locus_tag="ACHJWA_004210"
     CDS             276..1370
                     /gene="ddlA"
                     /locus_tag="ACHJWA_004210"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO4310692.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1394..1606)
                     /locus_tag="ACHJWA_004211"
     CDS             complement(1394..1606)
                     /locus_tag="ACHJWA_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFO4310693.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1866..2174
                     /locus_tag="ACHJWA_004212"
     CDS             1866..2174
                     /locus_tag="ACHJWA_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFO4310694.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2233..3327)
                     /gene="yaiW"
                     /locus_tag="ACHJWA_004213"
     CDS             complement(2233..3327)
                     /gene="yaiW"
                     /locus_tag="ACHJWA_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFO4310695.1"
                     /translation="MSRVNHLSSLSFLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSGIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAAKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3340..4560)
                     /gene="sbmA"
                     /locus_tag="ACHJWA_004214"
     CDS             complement(3340..4560)
                     /gene="sbmA"
                     /locus_tag="ACHJWA_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFO4310696.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFALFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4912..6069
                     /gene="ampH"
                     /locus_tag="ACHJWA_004215"
     CDS             4912..6069
                     /gene="ampH"
                     /locus_tag="ACHJWA_004215"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFO4310697.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6070..6693)
                     /gene="iprA"
                     /locus_tag="ACHJWA_004216"
     CDS             complement(6070..6693)
                     /gene="iprA"
                     /locus_tag="ACHJWA_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFO4310698.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6781..9723)
                     /gene="ehaB"
                     /locus_tag="ACHJWA_004217"
     CDS             complement(6781..9723)
                     /gene="ehaB"
                     /locus_tag="ACHJWA_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaB"
                     /protein_id="HFO4310699.1"
                     /translation="MHSWKKKLIVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLTIKNSTIHGMITSECMTTDCADDRATGYVYDRLTLSVDNSTIDDNYEHYTYNGTYN
                     NAADTHVVDVYDMGTAITLDQEVDLSITNNSHVAGITLTQGYEWEDIDDNTVSTGVNS
                     SEVFNNTITVKDSTVTSGSWTDEGTTGWFGHTGNASNYSNTLTADDVAIAAIANPYAD
                     NAMQTTVTLDNSTLMGDVVFSSNFDENFFPQGANSYRDADGDVDTNGWDGTDRMDVTL
                     NNGSKWVGAAMSVHMVDEDGDGSYDGYAVGTEATATLLDIAANSLWPSSTVGVDNINT
                     QYDENGHIVGNEVYQSGLFNVTLNGGSEWDTTKSSLIDTLSINSGSQVNVADSRLISD
                     TVSLTGGSNLNIGEDGHVATNTLTIDNSTVKMSDDVSAGWGLEDAALYANTITVTNDG
                     LLDINVDQFDANPFQADTLNLTSTTDTNGNIHAGVFDIHSSDYVMDTDLVNDRTNDTT
                     KSNYGYGLIAMNSDGHLTINGNGDNDNTASIEAGQNEVDNNGDHVAAATGNYKVRIDN
                     ATGAGSIADYNGNELIYVNDKNSNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTD
                     YANMALSIPSANTNIWNLEQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTINY
                     DQDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFA
                     NNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVTPY
                     GSISGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYV
                     YDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSA
                     NVGVKYTW"
BASE COUNT         2579 a         2453 c         2346 g         2572 t
ORIGIN      
        1 tttattataa ataaaagtta tgaaatatat tctcttcgtt aacttataat taagagaaaa
       61 aaactcaccg catggccatt taagccagga ataaattagc ttcccgttca tcatttttgt
      121 gatcttgcgc acatctttga gataacgcac tttcttcacc agtacgttgc acgctttgtc
      181 agcagaagat tgcaacaatc tttcagaagg ccgcgaatcc cccgttttgt cgtggtaatg
      241 tatgcacttt gtgttagacg ggataggttt ttaagatgga aaaactgcgg gtaggaatcg
      301 tttttggtgg taaatcagcg gaacatgaag tgtctctgca atcggcaaaa aacattgtcg
      361 atgccattga taaaagtcgc ttcgacgttg tgctgctggg cattgataaa caagggcaat
      421 ggcacgtcag cgatgccagc aattatctgc taaatgcaga cgatcctgcc catattgcgt
      481 tgcgcccttc ggcgaccagc ctcgcccagg tgccaggtaa acatgagcat cagcttatcg
      541 acgcgcaaaa cggtcagccg ttgccgacgg tggatgtcat tttcccgatt gtccacggta
      601 cgctgggcga agatggttcc ttgcagggaa tgctgcgggt cgccaattta ccgtttgtag
      661 gttctgatgt tctggcttca gcagcctgta tggataaaga tgtcaccaaa cgtctgctac
      721 gcgatgccgg gctgaacatt gcgccattta ttaccctgac gcgcgctaat cgtcacaaca
      781 tcagttttgc cgaagtggag tctaaactgg ggttaccact gtttgtaaaa ccggctaatc
      841 agggctcttc tgttggtgtc agcaaagtaa ccagtgaaga acaatacgca attgccgtcg
      901 atctggcgtt cgagttcgat cataaagtga tcgttgagca agggatcaaa ggtcgtgaga
      961 tcgaatgcgc agttctgggc aacgacaatc cgcaagccag cacctgtggc gagatcgtac
     1021 tcaccagcga tttctatgcc tacgacacca agtacattga cgaagatggc gcgaaagtgg
     1081 tagttccggc agccattgcg ccagaaatca acgataagat ccgggcgatt gccgttcagg
     1141 cttatcagac gttgggatgc gcaggcatgg cgcgtgtaga cgtgttttta accccagaga
     1201 acgaagtggt gatcaacgag atcaacacac tgcctggctt caccaacatc agtatgtatc
     1261 cgaagctgtg gcaagccagc ggtctgggtt acaccgatct gatcacacgt ttgattgagc
     1321 tggcgctgga gcgtcacgct gcggataacg cattgaaaac cacaatgtaa tattttaacg
     1381 cccgataata ttcttattgg gcgttttcag tttcttcatc gcgacgacga ataatcaacc
     1441 ccgccagcca gaaactaatc acccacgtca ccagaccgac ggcataggtc tgccaacctt
     1501 ttgcttcaaa tcccagtaac cccaccacgc cattaagaat gaatataagg ccaatggcga
     1561 acgcatagta gtgccagtca cggcggattt ttacaggcag attcatggct gctccgttaa
     1621 cgaaaaacaa catccttgca tattctgcaa caaccgtctg tggtgtagac aacattttcg
     1681 caattgttaa taaatttaac ttaaagttgc tattttgtga ctcgcaacga agattcacgc
     1741 ttcgggaata ttcccaacat caatttacca tgattctgat attgacaatc taaatgacct
     1801 gtcagacttg ccataagccg ctggtatcac accagaagag aagcagatga cttccggagg
     1861 tttttatggc tgatttcacc ctgtcaaaat cgctgtttag cggaaaatat cgcaatgcct
     1921 cttcaacgcc tggcaacatt gcctatgcgt tgtttgtgct gttttgcttt tgggctgggg
     1981 cgcaattgct gaacctgtta gtgcatgcgc ccggcgtcta tgagcgttta atgcaggtcc
     2041 aggaaacagg tcgcccacgg gtggaaattg gtttaggtgt cggcaccatt ttcgggctga
     2101 tcccgttttt agtaggctgc ctcatttttg cagtggtggc gctatggctg cactggcgac
     2161 atcgccgcca gtaagattta cgttgccaga tacggcctaa aacgccttat ctggcatgac
     2221 attcctgttt gttcactgtt tcatacaacg cgcccgccgt tcgtctacgc gcttcgcaaa
     2281 ccaggccgta gtcaggttgc gggtgatttt cgggctttcc agttgaatgc caggcaacat
     2341 ttcgcggggg agtgatttac ccgttttcgc ttcggcaagt tgataaactt tcttgtacag
     2401 cgccgtctcc tcaaaagaga agctgtcacc tttctctagc tgacgacgaa tttcgctgtc
     2461 gctcatcccc agttttgccg ccagtttgcg cgttgccagt tcggttttcc cgggttcttt
     2521 actgtcgtaa cgaattaaat caccgtccag tgccagcttc acgccgctgg ccttactgac
     2581 cgcgttctga aacgcggcat tacggctggc gtaccagcca gcgttaaaat cagcaaaacg
     2641 gtatatcggt gcgctataac tggcgggata attcagtaaa tggtaagtac caaaccacaa
     2701 cccgccgcgg cgactaaaga cttcctgacg gactgtaccg tccattttcc acggataccc
     2761 tttggtatgc tgttcggcaa aagcaatgct gacctgcatc ggcccaccgg tgcgcaccgg
     2821 attaagcgaa ccaaacaacg tctgccccat tggcaccatg ctgatcagat cgtcaaaaat
     2881 cccgctcaat tgcttttctg tacgcaccga atctaaccgt tcgctataac ttttgccgtt
     2941 tggcgatttg atttttagcg cagtatgaac cagaaaagca ggaatgtgca tccgttcagc
     3001 acgacggtca atctcttgcc aggcgatctt gcttaagccc ggaacggccg gatctgcctg
     3061 ataatttgac tcctgctgcg ccaccgccag caccgagcag acgttttcca gcgtcggtgc
     3121 cagcccctga ctttcaaagg tggtggcgag atctttcgcc caggcatcgc ggtctttcac
     3181 gctcgcaggc atcttctgcc gcaccacgct agccacatct atcgcttttt cgcccttttt
     3241 caacggctgt ggcgcctgac tgctacaccc cgctaaaacc agaaccgcca aaaatgaaag
     3301 cgaagacaga tgattgactc gcgacattac ttctcctttt tagctcaagg tatgggttac
     3361 ttcctgaatt ttgtcaccat ccagctcatg ttcaaagctg cgcagacgtt tgtagataga
     3421 catcaactca accagtgtgg tccatgagtt aatcaggtac tggaaagcac cgcgaacctg
     3481 accaaaaacg ttggtaatct gcgtcatcag gccgagcgta atcgtaccgg caacaattga
     3541 cggaaacagc aagaacaaac cgaaaacgtt atcgacctgc aaatagagaa tgcgagcgat
     3601 gttgaaatac atatagtgaa aatagaggcg gaaatagttt ttccgtacgg cgctaaacag
     3661 ctcgcgtacc gttggcggcg tcgcgcgcgt ggcatcgtct tcaccataaa ccagctcttt
     3721 acggtaggca gcctctacac gctggttttt aaactccagc cccggcagtt tgatccctac
     3781 cactgccagc aatccggtcc ccatcagcga ccagacgata gcggcaatca ccagaccata
     3841 cggaatgtgc ccgataatcg gcagctccgg cacatgcgcg gagagcgtta ccaacaccgg
     3901 caggaaggcg atcaacgtca tgatggcgtt gataaaactg acacccatat tctccagcgt
     3961 tgaagcaaaa cgcatggtgt cttcctgcac acgctgtgcg gcgccttcga tatgacgcag
     4021 ttgttgccag ttcgccatgt aatattcgtt catcgccgta cgccagcgga acacgtagtg
     4081 actgacaaag aagttattca gcacactgat cactaccgcg atcagcgcaa tccccagaaa
     4141 gacgcccact tcgcggtaaa attgttcgat agtgacttta tgcggcgaac ttagcgcggt
     4201 ttgaatcaga tcatagaacg gtgcatacca ggcgttgacg gcaaccccaa cttccaccaa
     4261 aaaccaggtg acgaagatga tcagtgcagt accgagtatt gaccagtatt gccaacggtg
     4321 cgggctgtag ataaaccaga acaatgcaaa aagtcctacg caaacaatgt agtaagcgta
     4381 aaaaatcagg aaatccaacg accagaaacg cgcggcgcta atcgggatct gcccggaagc
     4441 gccggtgata cgcgccaccc agtccccccc accggcttgc cagaagataa cggcaatcaa
     4501 tgcccaaaca aaggccgaga gaaaaaacgt tcccggcttt gggaaaaaag acttaaacat
     4561 atgcactcct gctaacttct tatcgtttta acaactgctg tgtattaacc gcatgaccgc
     4621 tacaaagcgt cagccaaatg aaaccgccca ttgtgactca tagcaaggcg cttagttcgc
     4681 cagcctggcc ataagaattg tttcgacgga tttcacaaac caaagattgt ttacataagc
     4741 ggattaacac gcgcaggatc acaaatgcag gcattggtga gcaggaaaaa gatcgtgttt
     4801 acgcgccgtt acgtttttaa cactattatt gcactaattc gccctttgca atccatcaat
     4861 gagtagtata aatacgctca gttaccttca ttcaatttat ggacaccacc gttgaaacgt
     4921 agtctgcttt tttctgccgt gctgtgtgcg gcgtcattga cctccgtcca tgcggcgcag
     4981 ccgatcactg aaccggagtt tgcctctgat attgtcgatc gttatgccga tcatattttt
     5041 tacggcagcg gtgccacggg gatggcgctg gttgttatcg acggtaacca gcgcgtcttt
     5101 cgcagttatg gcgaaacgcg acctggtaat aacgttcgcc cgcagctgga ttcggtcgtg
     5161 cgtattgctt ccctcaccaa gctaatgacc agtgagatgc tggtgaaatt gctcgaccag
     5221 gggaccgtga agttaaacga tccgctaagt aaatatgctc cgccaggcgc acgcgtgcca
     5281 acctacaatg ggacgcccat tacgctggtc aatctggcaa cccataccag cgccctgccc
     5341 cgtgaacagc ccggtggcgc ggcacatcgt ccggtgtttg tctggccaac gcgcgagcaa
     5401 cgctggaaat acctttctac ggcgaagctg aaagccgcgc caggcagtca ggcggcgtac
     5461 tctaaccttg cgttcgacct gctggcagat gcactggcga atgcgtccgg caagccttat
     5521 acccagttgt ttgaagagca gattacccgt ccattgggga tgaaagacac cacctacact
     5581 ccctcaccgg atcagtgccg ccgtttaatg gttgctgagc gcggtgccag tccgtgcaat
     5641 aacacgctgg cggcaattgg cagcggcggc gtctattcca cgcctggcga tatgatgcgc
     5701 tggatgcagc agtatttgtc atctgatttt tatcagcgca gtaaccaggc agatcgcatg
     5761 cagacgttga tttatcagcg cgcgcagttt acgaaagtga ttggcatgga tgtccccggc
     5821 aaagcggatg cgcttggttt aggctgggta tatatggccc cgaaagaggg tcgtccgggg
     5881 attattcaga agacaggtgg tggtggcggt ttcattacct atatggcgat gatcccgcag
     5941 aagaatattg gcgcgtttgt ggtggtcact cgttcgccgt tgacgcgctt taaaaatatg
     6001 agcgatggca tcaatgatct ggtgaccgag ctaagcggga ataaaccgtt ggttatcccc
     6061 gcatcctgat taatactctg aaggcaaacg gttgatagcg accagtttgc ctttattcat
     6121 ttcgatatag ccgcctttac gcaaagcagc gagaacttct gcgacgacag aacgcgatat
     6181 gcgtgtacgt tgatggatat aattcatcac accaatacgt gatcgcaatt cttcattcca
     6241 gtcaatcatt gataataatg ttgcgcggat ttgttcgtag gaattatgcc caatgagctg
     6301 cacgtcgcgt aattccagaa ttctattttg ccaggctaac cagtaaaaag cgtctcgcca
     6361 gagttgattt tgttcaataa gcgtaatggt ttgtttggct ggtagatgat atcccgtaca
     6421 atttccttct gatattaatt tgtatggtat atcgtttttc attaaaccat cagccagccc
     6481 cataatataa ggagcctggg taataccgat aagtacgttt tcttctctac gcagagagat
     6541 aactccctct aaaataacga aagtatcttc gttattgaca tcactggaaa atataacttg
     6601 cttttcatta ttaaactcga agcgcgtacc gtatctggac aaacatttat cgagcttacc
     6661 aaattcctga agaggtttaa ctacagataa catttgcgcg tcctttgcag taatgcccgt
     6721 caaatccttg acgggcatta tttagattaa attaccagta tttcttcgga gtgaagaata
     6781 ttaccaggta tatttaacac ccacgttcgc ggaccagtct tgatctacgt caccaccacc
     6841 gaggtagtta gcatcggtat aggcgctgaa gttcttggtg aagctgaact gagtacccag
     6901 accaacacgt accgcagacc cttcagtacc gttatcgatg gaatcaccgt tcacatcgtt
     6961 atcgttgtta gagtcgtcgt agacgtaagc cagtttgaag tacggagtca gagcctgatc
     7021 ttcgctgtag gtgaaggtat aacctgcatc tacacccagt tcataacgca tgctgtcgta
     7081 agactgaccg tcaactttca tgtcgttgct cagctggtag tcatcaccag actggaacag
     7141 accagaaatg ctgccgtaag gagtcacgta accagcatca cccagtttga agtcgtaacc
     7201 ggctttcaaa ccgaagcccc aagcgtcgga gttggtgcta ccgtcaacgt aagtaccgtt
     7261 gctcatggtt gcagacaggt cgttgttgaa gtgagagtag ctcaagctac catcaacaaa
     7321 gacgttgttc gcgaagtgag cagaagagta gatgtaggca gtctggctgt cttgatccac
     7381 ctgaccagaa cggtcattca tgtcaccttt agcgaagcct gcagccgcac cgacgatcca
     7441 cttagcgttg ttaccgtcaa ttttggtatc aacaccgacc atgatgccgt taacatcctg
     7501 atcatagttg atggtgccgt tgtcgccgtt gaagttacca ccgaagtagc ttacccatgc
     7561 gccgccgtta tcagccaggc catgacgaga gttggtcaaa cgagtaccaa cggtgtcttg
     7621 ttccaggttc cagatattgg tgttcgcaga tgggatgctc agcgccatgt tagcgtagtc
     7681 ggtcaactcc atctgttgca gaacaacggt gttaccgcgc tgttcagcct gataggtgta
     7741 tgcacccagg tcagctttgt tagcagcaga gaaggtcgcg ttgctgtttt tgtcgttgac
     7801 gtagatcagc tcgttgccgt tgtagtcagc gatagaacca gcaccagtag cgttgtcgat
     7861 acgaactttg tagttaccgg tcgcggctgc aacatggtca ccgttgttat caacttcgtt
     7921 ctgaccagct tcgatagaag cagtgttgtc gttatcgccg ttaccgttaa tagtcaggtg
     7981 accatcagag ttcattgcga ttaagccata accgtagttt gacttggtag tatcgttggt
     8041 acgatcgttg accagatcgg tatccattac gtaatcactg ctatggatat cgaatacacc
     8101 agcgtgaatg ttgccgttag tatcagtggt actggtcaga ttcagggtat cggcctggaa
     8161 cgggttagca tcgaactgat caacgttaat atccaacaga ccgtcgttag ttacggtgat
     8221 ggtatttgcg tacagtgcag catcttctaa accccagccc gcagaaacat catcagacat
     8281 tttaacggta ctattgtcga tggtcagggt attagtcgct acatgaccgt cttcaccgat
     8341 gttcaggtta gaaccgccag tcagagagac agtgtcagag atcagacgag agtctgcaac
     8401 attaacttgg gaaccgctgt taatacttaa agtatcaatc agagaagatt ttgttgtatc
     8461 ccactctgaa ccaccgttca aagtcacatt aaacaaaccg ctctggtaaa cttcgtttcc
     8521 tacgatatgg ccattttcgt catattgagt attgatgtta tcaacaccga cagttgatga
     8581 aggccacagg ctgttagctg caatatcgag cagagttgca gttgcttcag taccaacagc
     8641 atatccgtcg taagaaccat caccatcttc atcaaccata tgaacagaca ttgcagcgcc
     8701 aacccacttg ctgccgttgt tcagagtcac atccatacgg tctgtgccat cccaaccgtt
     8761 ggtatctaca tcaccatcag catcgcgata gctgttagca ccttgcggga agaagttttc
     8821 atcgaaatta ctggagaaaa caacatcacc catcagtgtt gagttgtcta aagttactgt
     8881 agtctgcatc gcattatcag catacggatt tgcgattgcg gcaattgcaa catcgtctgc
     8941 agtcagcgtg ttgctatagt tgctggcatt accagtatgg ccaaaccaac cagtagtacc
     9001 ttcatcagtc catgaaccag aggtcacagt agaatcttta acagtaatag tgttattaaa
     9061 cacttcgctg ctgtttacgc cagtgctgac tgtgttgtcg tcaatatctt cccactcata
     9121 accctgagtc agcgtaatac ctgctacatg agagttatta gtgatggaca gatcaacttc
     9181 ctgatccagt gtaatagcag tacccatatc gtaaacatct acaacatgag tgtcagcggc
     9241 attattatag gtaccgttgt aagtataatg ctcgtagttg tcatcgatcg ttgaattatc
     9301 aacgctcagt gtcagacgat cataaacata accagtagca cggtcatcag cacaatcagt
     9361 agtcatgcac tcagaggtaa tcataccgtg aatagtgctg tttttgattg tcagactatt
     9421 ggcgttggta ttggtagaaa taccgtcgtc cagatagtag gtagaaatca cgccgttaac
     9481 ggtagcgtta ttaatgaccg ggtagatatc gccgttgtaa tagctatcag cggcatagtt
     9541 gttccagcct acataaccat catagtaaac gtcatcagca taggtggcgt cgttgtagtg
     9601 atggaaagta ttgtaagttt gaccagaaat atcgttggtc gctgcattag cctgagaggt
     9661 gatagccaga gtgcaagcca atgctaattg tgatactata agtttctttt tccaggagtg
     9721 catttgtcat ccctcctcag ggacgtaata agttgatcca tcaattatca atgcatagaa
     9781 aacatcttat gtttcgcgtt gacgattatg cggcgttata gaggaatggt tcaattattc
     9841 tttcccctaa gtccgattgc ggacatatat aaaaatatct atacgcacaa agaataagtt
     9901 gacaaaaaca ctgcatttca acacgttaat gaaatcatta attttgaatt
//
LOCUS       DAZFAB010000141         9926 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZFAB010000141 DAZFAB010000000
VERSION     DAZFAB010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9926
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.141"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..1332)
                     /gene="rhsB"
                     /locus_tag="ACHJWA_004218"
     CDS             complement(<1..1332)
                     /gene="rhsB"
                     /locus_tag="ACHJWA_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsB"
                     /protein_id="HFO4310700.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQ
                     ALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEVLPAPLPPYRVLTGLVDRFGR
                     TQTFHREAAGEFSGEITGVTDGAGRHFRLVLTTQAQRAEEARQQAISGGTEPSAFPDT
                     LPGYTEYGRDNGIRLSAVWLTHDPEYPENLPAAPLVRYGWTPRGELAVVYDRSGKQVR
                     SFTYDDKYRGRMVAHRHTGRPEIRYRYDSDGRVTEQLNPAGLSYTYQYEKDRITITDS
                     LNRREVLHTQGEGGLKRVVKKEHADGSVTQSQFDAVGRLRAQTDAAGRTTEY"
     gene            complement(1492..1704)
                     /gene="rpmE"
                     /locus_tag="ACHJWA_004219"
     CDS             complement(1492..1704)
                     /gene="rpmE"
                     /locus_tag="ACHJWA_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFO4310701.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            1907..4105
                     /gene="priA"
                     /locus_tag="ACHJWA_004220"
     CDS             1907..4105
                     /gene="priA"
                     /locus_tag="ACHJWA_004220"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFO4310702.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMAVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDSEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGHAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            4261..5286
                     /gene="cytR"
                     /locus_tag="ACHJWA_004221"
     CDS             4261..5286
                     /gene="cytR"
                     /locus_tag="ACHJWA_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFO4310703.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            5378..6337
                     /gene="ftsN"
                     /locus_tag="ACHJWA_004222"
     CDS             5378..6337
                     /gene="ftsN"
                     /locus_tag="ACHJWA_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFO4310704.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVTRAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            6430..6960
                     /gene="hslV"
                     /locus_tag="ACHJWA_004223"
     CDS             6430..6960
                     /gene="hslV"
                     /locus_tag="ACHJWA_004223"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFO4310705.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            6970..8301
                     /gene="hslU"
                     /locus_tag="ACHJWA_004224"
     CDS             6970..8301
                     /gene="hslU"
                     /locus_tag="ACHJWA_004224"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFO4310706.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            8368..9294
                     /gene="menA"
                     /locus_tag="ACHJWA_004225"
     CDS             8368..9294
                     /gene="menA"
                     /locus_tag="ACHJWA_004225"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFO4310707.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVVGIFLSQWAA"
     gene            9387..9872
                     /gene="rraA"
                     /locus_tag="ACHJWA_004226"
     CDS             9387..9872
                     /gene="rraA"
                     /locus_tag="ACHJWA_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFO4310708.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
BASE COUNT         2309 a         2675 c         2751 g         2191 t
ORIGIN      
        1 gtattctgtt gtcctgcctg cggcatccgt ctgtgccctg agcctgccca ccgcgtcaaa
       61 ctgactctgc gtgacgctgc cgtccgcgtg ttcctttttc accacccgct tcagcccgcc
      121 ttcaccctgc gtgtgcagga cttcacggcg gttcaggctg tcggtgatgg tgatgcggtc
      181 tttctcatac tgatacgtgt agcttaagcc tgccgggttt agctgttctg tcacccgccc
      241 gtcgctgtcg taacggtaac ggatttccgg ccggcccgtg tgacggtgcg ccaccatccg
      301 gccccggtat ttatcatcgt aagtaaagct gcgcacctgt ttgccactac ggtcatacac
      361 caccgccagt tcgccgcgcg gcgtccagcc atagcgcacc agcggcgcgg caggtaaatt
      421 ctccgggtat tccgggtcgt gcgtcagcca cacggcagac agacggatgc cgttgtcccg
      481 gccatattcg gtgtaacccg gcagggtatc aggaaaagcg gacggttccg tcccgccgga
      541 aatggcctgc tgccgggctt cttctgcccg ctgcgcctgc gtggtcagta ccagccggaa
      601 gtgacgcccg gcaccatccg tcacgccggt gatttcgccg ctgaattcac cggcggcttc
      661 gcggtggaac gtctgtgtgc gcccgaagcg gtccaccagc ccggtcagta cccggtacgg
      721 cggcagcggc gcaggcagca cctcatccgc ttccggcacc cgctcacacc agccgagcag
      781 ccaccacggc ccctgcggac tgtttgtcgc cagataacga tgcggactta agcggagttc
      841 ttccggcagc gcctgccaga gtgcggccag ccggtgacct tcatccagtt tcgccacgcc
      901 gccgcgcacc agccagagtg actcgctgcg gctgtaaccg tcctcaccgg gaaacaggtg
      961 ctcaaaatac aggcttctgc cgccgttatc actgagtatc agtgtgttat cgcgcagctg
     1021 taagcggata tccgcaggca ttttccagcc ggggccgagg ctccccaccg gcgcgggcgt
     1081 ttttgtccgg taactgctgt aggtgcggga gaggatgaac ggcagcgggc cgggcagggc
     1141 gatgtcggtt tcaccgggaa ggacctttgc accgagcagg ggattgaccg gatggccgga
     1201 cgtcactccg ccggggcaca ccgaacaggc cacgccggtg ggggcaccaa tgcgcacccc
     1261 ggctgaaccc tgaacaatgc taccgccata ctgcgtcatg tcgccctgac gcgccgccgg
     1321 ttttccgctc atcgtaaaat ccttttgaaa taaataagaa ccagaagcct agcgccagtc
     1381 aacaacagga aatgataatg agttaacacc ccggaatttt tcagaattta taaaaggcaa
     1441 attacaggca caaaaaaagc gccgtgcggc gcttttttcg gaaatccggt cttatttgct
     1501 gcccgggatg ttgaaacgct tgttgaagcg gtcaacacgg ccaccggtag caacatcacg
     1561 ctgtttgcca gtgaagaacg ggtggcactt gctgcacacg tcgaggttca ggtcatgacc
     1621 aacggtggag cggattttca ttacgttacc gcaagagcag ctagcagtaa tttcttcgta
     1681 tttcgggtga atatcttttt tcatgggaaa acctcggttt aaggccgcgt cgctcttcca
     1741 gccctaacgc cagacaccac gcgatgttaa aagtatagct tcaatacgat catttcgtac
     1801 gaaggcgcga aatcatacag aaattaacca gcatatgcaa actgatccgc actcttctac
     1861 ggcaatgtgt atactaaccc accgaatttc aagtcaggat gatgctatgc ccgttgccca
     1921 cgttgccttg cccgttccgc ttcctcgtac ctttgactat ctcctgccag aaggcatggc
     1981 ggttaaagcc gggtgtcgcg tgcgcgtgcc gtttggcaaa cagcaggagc gcatcggggt
     2041 tgtggtgtca gttagcgacg tcagtgagct gccgcttaac gagctaaaag cggtagtcga
     2101 agtgctggat agtgagccgg tgtttactca ctccgtctgg cgattgctgc tctgggcggc
     2161 ggattactat catcatccga ttggcgatgt gctgtttcat gccttgccga ttttactgcg
     2221 ccaggggcgg cctgcggcga acgcgccgat gtggtactgg tttgccactg aacaaggcca
     2281 tgcggtggat ctgaacagcc tgaaacgttc gccaaagcaa caacaggcac tggcggcatt
     2341 gcggcaaggc aaaatctggc gcgaccaggt tgccacgctt gaatttaatg atgccgcgtt
     2401 acaggcgctg cgcaaaaaag gtctgtgtga tttagcaagt gaaacaccag agtttagcga
     2461 ctggcgaacg aactatgccg tttctggtga acggttgcga ttgaataccg aacaggccac
     2521 cgccgttggc gcaattcata gcgcggcgga taccttctct gcttggctgc tggcgggcgt
     2581 tacaggttcc ggtaaaacag aggtttattt gagcgtactg gaaaacgtgc tcgctcaggg
     2641 caaacaggcg ctggtgatgg tgccggaaat cggcctgaca ccgcaaacta tcgcccgttt
     2701 tcgtgaacgt tttaatgccc ccgtagaagt tctgcattcc ggcctgaacg acagcgagcg
     2761 tctttcggcg tggttgaaag cgaaaaatgg tgaggcggcg attgtcatcg gcacccgctc
     2821 cgcgctgttt acgccgttta aaaatctcgg tgtgattgtc attgatgaag agcacgacag
     2881 ttcctacaag cagcaggaag gctggcgcta tcatgcccgc gacctggcgg tttatcgtgc
     2941 gcacagcgag caaatcccga ttattcttgg ctccgcaaca cccgcactgg aaacgttatg
     3001 caacgtccag cagaaaaaat accgcctgct gcgcctgacg cgtcgggcgg ggaatgcgcg
     3061 tccggcaatt caacatgtgc tggatttaaa aggtcagaag gtgcaggcgg ggctggctcc
     3121 ggcgttaatc acccgtatgc gccagcattt acaggccgac aaccaggtta tcctctttct
     3181 taaccgccgt ggctttgcgc ctgccttgct gtgccacgac tgcggctgga ttgccgaatg
     3241 tccacgttgc gatcactact acacgctgca tcaggcgcaa cagcatttgc gctgtcacca
     3301 ctgtgacagc caacgcccgg tgccgcgcca gtgcccttcc tgcggttcca cgcacctggt
     3361 acccgtgggg ctgggcaccg aacagcttga acagacgctc gcgccgttat tccctggtgt
     3421 gcccatttct cgtatcgacc gcgataccac cagccgcaaa ggagcgctgg aacaacaact
     3481 ggcggaagtg catcgcggcg gcgcgaggat attgattggc acgcaaatgc tggcgaaagg
     3541 tcaccacttt ccggatgtga cgctggtagc gttactggat gtggacggcg cgctgttttc
     3601 tgccgatttt cgctcggcag agcgttttgc ccagctatac acccaggtag caggccgtgc
     3661 cgggcgtgcg ggtaaacagg gcgaagtggt gctgcaaacg catcatccgg aacacccgct
     3721 attgcaaacg ttgctctata aaggctacga cgcttttgcc gaacaggcac tggctgagcg
     3781 gcgaatgatg caactgccgc cgtggaccag ccatgtgatt gtgcgtgcgg aagatcataa
     3841 caatcagcac gcgccattgt tcctgcaaca actgcgtaat ctgatcctct ccagcccgct
     3901 ggcagacgac aaattgtggg ttctcggtcc ggttccggca ctggcaccta aacgtggcgg
     3961 tcgctggcgc tggcagatat tgttgcagca cccttcccgc gtgcgcttgc aacacatcat
     4021 taacggtacg ctggcactca tcaatacaat accggattcc cgtaaggtga aatgggtgct
     4081 ggatgttgat ccgattgagg gttaaaccgc tcacgatgcg aggcggatcg aaaaattcaa
     4141 tattcatcac acttttcatg aaaattctgt aaccgttttc acgcgctatc tgctaaaaat
     4201 gttgccgatg tgaagtaaac atggatgtag tacgcctgac gtgccaggcg aggagtgagt
     4261 gtgaaagcga agaagcagga aactgccgcg accatgaaag acgttgccct caaggcaaaa
     4321 gtctctacag cgaccgtctc ccgagcatta atgaatcccg ataaagtctc ccaagccacc
     4381 cgtaatcggg ttgaaaaagc ggcccgtgaa gtgggttatt taccgcagcc tatggggcgc
     4441 aacgtcaagc gtaatgaatc ccgcaccatt ctggtgattg tcccggatat ctgcgatccc
     4501 ttctttagcg aaattattcg cggtatcgaa gttacggcgg caaatcacgg atatctggtg
     4561 ctgattggcg actgtgcgca tcaaaatcag caggaaaaaa cctttatcga tttgatcatc
     4621 accaagcaaa ttgatggcat gttgctgctg ggttcaaggc tgccgtttga tgccagcatt
     4681 gaggaacagc gtaatctgcc gccgatggtg atggcgaacg aatttgcacc ggaactggag
     4741 ctgcctaccg ttcatatcga caatctgacc gccgcatttg atgcagtaaa ttatttatat
     4801 gagcaagggc ataaacggat tggctgtata gccggtcccg aagagatgcc gctgtgtcac
     4861 taccgcctgc aaggttacgt tcaggcgctg cgtcgctgcg gcattatggt tgatccgcaa
     4921 tacatcgccc gtggcgactt caccttcgaa gccggaagca aagcgatgca gcagctgctt
     4981 gatcttccac aaccgcctac tgctgtcttc tgccatagcg atgtgatggc gctcggcgca
     5041 ctttctcagg caaaacgcca ggggctgaaa gtcccggaag acctttccat aatcggtttt
     5101 gataacatcg acctgacgca attttgtgat ccgccgctga caaccatcgc gcagccgcgt
     5161 tacgaaatcg gtcgggaagc tatgctgtta ttgcttgatc aaatgcaggg gcaacacgtt
     5221 ggcagtggct ctcgtttaat ggactgcgaa cttatcatcc ggggatcaac acgcgcgtta
     5281 ccttaaagta aacctttaag actgccgtga ctggtcaaag gcccgccgct taagtaacat
     5341 ggcgggctga cgaacgaata aatacagcga aacgatagtg gcacaacgag attatgtacg
     5401 ccgcagccaa ccggcacctt cgcggcgaaa aaagagcacc tcacggaaaa agcaacgaaa
     5461 tctgcctgcg gtttctcccg ctatggtcgc tattgctgcc gccgttcttg tgacctttat
     5521 cggtggtctg tacttcatta cgcatcacaa gaaagaagag tccgagacgc tgcaaagcca
     5581 gaaagtgacc ggaaacggac taccaccaaa accagaagaa cgctggcgct acatcaaaga
     5641 gctggaaagt cgccagccgg gtgtgcgtgc gcccacagaa ccttctgccg gtggtgaagt
     5701 gaaaacgccg gagcaactga caccagaaca acgtcagctt cttgaacaaa tgcaggctga
     5761 tatgcgccag cagccaacgc agctggttga agttccgtgg aatgaacaga cgccggaaca
     5821 gcgtcagcaa acgctacagc gccaacgtca ggcgcagcaa ttagcggaac agcaacgcct
     5881 ggctcagcag tcgcgtacga ctgaacaaag ctggcagcag cagacgcgta cgtcgcaagc
     5941 cgcgccggta caggcgcaac cgcgccagtc taaaccggct tctactcagc agccgtacca
     6001 ggatctgctg caaacccctg cgcacacgac cgcgcagtcg aagccgcagc aagctgcgcc
     6061 ggttactcgt gccgctgacg cgccaaaacc gacggcggag aaaaaagacg aacgccgctg
     6121 gatggtgcag tgcggttcgt tcagaggcgc ggaacaggca gagacggtac gtgctcagct
     6181 ggcgttcgaa ggctttgact caaaaattac caccaacaat ggctggaatc gtgtggtcat
     6241 tggcccggtg aaaggcaaag agaacgcaga cagcaccctc aatcggttga agatggcggg
     6301 tcatacaaac tgcattcggc tcgccgccgg gggttgaaac cctcaaaatc ccccccatct
     6361 ataattgcat tatgccccgt acttttgtac ggggtttgta ctctgtattc gtaaccaagg
     6421 ggtcagctcg tgacaactat agtaagcgta cgccgtaacg gccatgtggt catcgctggt
     6481 gatggtcagg ccacgttggg caataccgta atgaaaggca acgtgaaaaa ggtccgccgt
     6541 ctgtacaacg acaaagtcat cgcgggcttt gcgggcggta ctgcggatgc ttttacgctg
     6601 ttcgaactgt ttgaacgtaa actggaaatg catcagggcc atctggtcaa agccgccgtt
     6661 gagctggcaa aagactggcg taccgatcgc atgttgcgca aacttgaagc actgctggca
     6721 gtcgcggatg aaactgcatc gcttatcatc accggtaacg gtgacgtggt gcagccagaa
     6781 aacgatctta ttgctatcgg ctccggcggc ccttacgccc aggctgcggc gcgtgcactg
     6841 ttagaaaata ctgaacttag tgctcgtgaa attgctgaaa aggcgttgga tattgcaggc
     6901 gacatttgca tctataccaa ccatttccac accatcgaag aattaagcta caaagcgtaa
     6961 ggatctccca tgtctgaaat gaccccacgc gaaatcgtca gcgaactgga taagcacatc
     7021 atcggccagg acaacgccaa gcgttccgtg gcgattgctc tgcgtaaccg ctggcgtcgc
     7081 atgcagctca acgaagagct gcgccatgaa gtgaccccga aaaatatcct gatgatcggc
     7141 ccgaccggtg tcggtaaaac tgaaatcgcc cgtcgtctgg ctaagctggc gaatgcgccg
     7201 ttcatcaaag ttgaagcgac caaattcacc gaagtgggct acgtcggtaa ggaagtggat
     7261 tctattattc gcgatctgac cgatgccgcc gtgaaaatgg tacgcgtcca ggctatcgag
     7321 aaaaaccgtt atcgcgctga agaactggca gaagaacgta ttctcgacgt gctgatccca
     7381 cctgctaaaa acaactgggg acagaccgaa cagcagcagg aaccgtccgc tgctcgtcag
     7441 gcattccgca aaaaactgcg tgaaggccag cttgatgaca aagaaatcga gatcgatctt
     7501 gccgcagcac cgatgggcgt tgaaattatg gctcctccag gcatggaaga gatgaccagc
     7561 cagctgcagt ccatgttcca gaacctgggc ggtcagaagc aaaaagcgcg taagctgaaa
     7621 atcaaagacg ccatgaagct gctgattgaa gaagaagcgg cgaaactggt gaacccggaa
     7681 gagctgaagc aagacgctat cgacgctgtt gagcagcacg ggatcgtgtt tatcgacgaa
     7741 atcgacaaaa tctgtaagcg cggcgagtct tccggtccgg atgtttctcg tgaaggcgtt
     7801 cagcgagacc tgctgccgct ggtagaaggt tgcaccgttt ccaccaaaca cgggatggtc
     7861 aaaactgacc acattctgtt tatcgcttct ggcgcgttcc agattgcgaa accgtctgac
     7921 ctgatcccgg aactgcaagg tcgactgcca atccgcgttg aactgcaggc actgaccacc
     7981 agcgacttcg agcgtattct gaccgagccg aatgcctcta tcaccgtgca gtacaaagca
     8041 ctgatggcga ctgaaggcgt aaatatcgag tttaccgact ccggtattaa acgcatcgcg
     8101 gaagcggcat ggcaggtgaa cgaatctacc gaaaacatcg gtgctcgtcg tttacacact
     8161 gttctggagc gtttaatgga agagatttcc tacgacgcca gcgatttaag cggtcaaaat
     8221 atcactattg acgcagatta tgtgagcaaa catctggatg cgttggtggc agatgaagat
     8281 ctgagccgtt ttatcctata atcgcgttca atcattttca tcattgtttg atggggctga
     8341 aaggccccat ttttattggc gcgtattatg actgaacaac aaattagccg aactcaggcg
     8401 tggctggaaa gtttacgacc taaaaccctc cccctcgcct ttgctgcaat tatcgtcggg
     8461 acggcgctgg catggtggca aggtcatttt gatccgctgg tcgccctgct ggcactgatt
     8521 accgccgggc tattacagat cctttctaac ctcgccaatg attacggcga tgcggtaaaa
     8581 ggcagcgata aacctgaccg tatcggaccg ctacgcggca tgcaaaaagg ggtcattacc
     8641 cagcaagaga tgaaacgggc gctcattatt accgtcgtgc tcatctgtct ctccgggctg
     8701 gcactggttg cagtggcgtg ccatacgctg gccgattttg tcggtttcct gattcttggc
     8761 gggttgtcga tcattgccgc tatcacctac accgtgggca atcgtcctta tggttatatc
     8821 ggtctgggtg atatttccgt actggttttc tttggctggt taagcgtcat ggggagctgg
     8881 tatttacagg ctcatacatt gattccggca ctgatccttc cggcgaccgc atgcggcctg
     8941 ctggcaacgg cagtactgaa cattaataac ctgcgtgata tcaatagcga ccgcgaaaac
     9001 ggcaaaaaca cgctggtggt gcgcttaggt gaagtgaacg cgcgtcgtta tcatgcctgc
     9061 ctgctgatgg gctctctggt atgtctggcg ctgtttaatc tcttttcgct gcatagcctg
     9121 tggggctggc tgttcctgct ggcggcaccg ttgctggtga agcaagcccg ttatgtgatg
     9181 cgggaaatgg acccggtggc gatgcgaccg atgctggaac gcactgtcaa gggagcgtta
     9241 ctgactaacc tgctgtttgt tgtagggata ttcctaagcc agtgggcagc ataactgaca
     9301 aatatcaatt aacaattgat gattttgcca acagaccaca tagcgcgata tactgaaaat
     9361 tctcgcagca actgaatgtt aagcctatga aatacgatac ttccgagctt tgtgacatct
     9421 atcaagaaga tgttaacgtc gtggaaccgc tgttctccaa ctttggcgga cgggcgtcgt
     9481 ttggcggaca aataatcacg gtaaaatgtt tcgaggacaa cgggttgctg tacgatctgc
     9541 tcgaacagaa tggccgtggt cgtgttcttg tcgtcgatgg cggtggttct gttcgtcgcg
     9601 cactggtcga tgctgaactg gcgcgtctgg cagtacaaaa tgaatgggaa ggtctggtca
     9661 tttacggcgc ggtgcgtcag gtagatgacc tggaagagtt ggatatcggc atccaggcga
     9721 tggcggcaat tccggttggt gccgctggcg aaggcattgg cgaaagcgat gtccgcgtca
     9781 attttggcgg tgttaccttc ttctccggcg accatcttta tgccgacaat accgggatta
     9841 ttctttcaga agatccgctg gatattgaat aagtatctgc catgcgttaa aaggggaatt
     9901 aaattcccct tttttataca tggacg
//
LOCUS       DAZFAB010000142         9765 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZFAB010000142 DAZFAB010000000
VERSION     DAZFAB010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.142"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            268..687
                     /locus_tag="ACHJWA_004227"
     CDS             268..687
                     /locus_tag="ACHJWA_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFO4310709.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            687..1955
                     /gene="umuC"
                     /locus_tag="ACHJWA_004228"
     CDS             687..1955
                     /gene="umuC"
                     /locus_tag="ACHJWA_004228"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFO4310710.1"
                     /translation="MFALCDVNAFYASCETVFRPELWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(2001..2531)
                     /gene="dsbB"
                     /locus_tag="ACHJWA_004229"
     CDS             complement(2001..2531)
                     /gene="dsbB"
                     /locus_tag="ACHJWA_004229"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFO4310711.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(2677..4218)
                     /gene="nhaB"
                     /locus_tag="ACHJWA_004230"
     CDS             complement(2677..4218)
                     /gene="nhaB"
                     /locus_tag="ACHJWA_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFO4310712.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            4440..5159
                     /gene="fadR"
                     /locus_tag="ACHJWA_004231"
     CDS             4440..5159
                     /gene="fadR"
                     /locus_tag="ACHJWA_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFO4310713.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(5211..6743)
                     /locus_tag="ACHJWA_004232"
     CDS             complement(5211..6743)
                     /locus_tag="ACHJWA_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFO4310714.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSVELLERCPPRMG
                     NL"
     gene            7106..8404
                     /gene="dadA"
                     /locus_tag="ACHJWA_004233"
     CDS             7106..8404
                     /gene="dadA"
                     /locus_tag="ACHJWA_004233"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFO4310715.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            8414..9484
                     /gene="dadX"
                     /locus_tag="ACHJWA_004234"
     CDS             8414..9484
                     /gene="dadX"
                     /locus_tag="ACHJWA_004234"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFO4310716.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEMYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
BASE COUNT         2437 a         2401 c         2607 g         2320 t
ORIGIN      
        1 aatgtcttta tttctattaa tatgataaat atcaaacaat gtttaatgtc attatggcga
       61 atgcttctat tctatttttt agccgggtga tatttttcat ttctgctgga tgagcgtcgt
      121 cgccagaagg ccgcgtgagc acaggataag agaacgaaaa atcagcagtc tatgcagcga
      181 caaatattga tagcctgaat cagtattgat ctgctggcaa gaacagacta ctgtatataa
      241 aaacagtata acttcaggca gattattatg ttgtttatca agcctgcgga tctccgcgaa
      301 attgtgactt ttccgctatt tagcgatctt gttcagtgtg gctttccttc accggccgca
      361 gattacgttg aacagcgcat cgatctgaat caactgttga tccagcatcc cagcgcgact
      421 tacttcgtca aagcaagtgg tgattctatg attgatggtg gaattagtga cggtgattta
      481 ctgattgtcg atagcgctat taccgccagc catggtgata ttgtcatcgc tgctgttgac
      541 ggcgagttta cggtgaaaaa attgcaacta cgcccgacgg tacagcttat tcccatgaac
      601 agcgcgtact cgcccattac catcagtagt gaagatacgc tggatgtctt tggtgtggtg
      661 atccacgtcg ttaaggcgat gcgctgatgt ttgccctctg tgatgtaaac gcgttttatg
      721 ccagctgtga gacggtgttt cgccctgagt tatggggtaa accggtggtt gtgctatcga
      781 ataatgacgg ttgcgttatc gcccgaaacg ctgaggcaaa ggcgcttggc gttaaaatgg
      841 gcgatccctg gttcaaacaa aaagatctgt ttcgtcgctg tggcgtggtt tgctttagca
      901 gcaattatga gctttacgca gacatgagca atcgggtgat gtcgacgctg gaagaactat
      961 cgccccgcgt cgagatttac agtattgatg aggcattctg cgatctgaca ggtgtgcgta
     1021 attgtcgcga tctgactgac tttggcagag aaattcgcgc aacggtgcta caacgtaccc
     1081 atcttactgt tggtgtgggg atcgcccaga ccaaaacgct ggctaagctt gccaatcatg
     1141 cggcaaaaaa atggcagcgg cagacgggtg gggtggtgga tttatcaaat ctggaacgcc
     1201 agcgtaaatt aatgtctgct ctccccgtgg atgacgtctg ggggattgga cggcggatca
     1261 gcaaaaaact ggacgcgatg gggatcaaaa ccgttctcga tttggcggat acagatatcc
     1321 ggtttatccg taaacatttt aatgtcgtgc tcgaaagaac ggtgcgtgaa ctgcgcggtg
     1381 aaccctgttt gcaactggaa gaatttgcac cgacaaagca ggaaattatc tgttcccgct
     1441 cgtttggtga acgcatcacg gattatacgt cgatgcggca ggccatttgt agttacgctg
     1501 cccgggcggc ggaaaaactt cgcagtgagc atcaatattg tcggtttatc tccacattta
     1561 ttaagacgtc accatttgcg ctcaatgaac cttattacgg caatagcgcg tcggtaaaac
     1621 tgctgacgcc cactcaggac agcagggata tcattaacgc cgctacgcga tctctggatg
     1681 ccatctggca agcgggccat cgctaccaaa aagcgggcgt gatgctgggg gatttcttca
     1741 gtcagggagt cgcgcagctc aatttattcg atgacaacgc tccgcgcccc gggagtgagc
     1801 aattgatggc ggtaatggat acactgaatg ctaaagaggg cagaggaaca ctctattttg
     1861 ccgggcaggg gatccagcaa caatggcaga tgaagcgagc catgctttca ccacgttata
     1921 caacgcgaag ttctgattta ctgcgggtca aataaacaca gcggcaggaa aaaagcgctc
     1981 ccgcaggagc gccgaatgga ttagcgaccg aacaggtcac gttttttcgc tttaaacggc
     2041 tgggaaatca ccaccagcac cgcgacaatc aggtaagcga taaaaatacc gagcagccac
     2101 tgcggcattt ccagacctaa aaaatcccac tgacgctcgg cgcaatcgcc agaggcgaca
     2161 aacacttgcg gcacccattt atccagcggc agccattccg ggaaacgaac cataaaatca
     2221 caggtggcaa acggcgaagg atagagctga agcatggtgt gctcgtaagt taactgcaca
     2281 ccgcggaacg cactatacaa ccagataacc atcgctacat aacgcagtgg cgtttttggg
     2341 gcgatcgcgc caatcagcgc agcacccaga acgccgaata atgcgcagcg ttcataaata
     2401 cagagcacgc aaggtttcag taacatcaca tgctggaacc acagcgccgt cagttccagt
     2461 gccagagcag taaacgccat caacagccac gcgccccggc cttgtgaaca ttggttcaaa
     2521 aatcgtaaca taatcatttc cctgcaatat gcatagagtg cgcagtttaa accaattcat
     2581 tcgctgcgcc accaggaggc agcaaaaata ggacgcaatt ggacaattat cgggcgaaaa
     2641 ggcaaaccgg gcatttatat gcccggtaag ttgttatcaa agcgttgcta tccagcccat
     2701 ttgcataaac cattcggtta caggggcaag cgtaaactcg acgcagagca gtccgacgag
     2761 tgtcaggaca agggtgtaag gcagggccat ccacaccatg cggccataag agaggcgaat
     2821 caatggcgcg agtgcagagg tcagcaggaa caggaacgca gcctgaccgt tcggcgtagc
     2881 gacagagggc agattggtac cggtattaat ggcgaccgcc agcagctcgt attgcttcaa
     2941 cgtgatagcg ccactttcca ttgccgcttt cgcttcgttg atataaatcg tccccacgaa
     3001 gacgttatcc gaaatggatg acagcagacc gttgaaaata tagaacagcg acagctgagc
     3061 atgttccgat gcctgcaaca caaactgaat aatcggcgaa aacagttgtt ggtcgataat
     3121 caccgcgact accgagaaaa acaccgtcaa cagtgcggtg aatggcagag attcggtgaa
     3181 ggctttaccg atagcatgtt catcggtgac accggtcaat gatgttgcca gaataatgac
     3241 tgacaaacca atcaagccaa cttccgccag atgcaacgcc agcgcagtca ccagccagac
     3301 gccaataatc gcctggacaa tcagacggat tttatcctga cgggtacgct ggtggcggct
     3361 ttgatcgtca aactgttgca acacttcgcg gactttctcc ggcagcgttt caccgtagcc
     3421 aaaccaacgc agcttctcta ccagcaggca ggttaacagg ccacaaatca gaaccggaac
     3481 ggtcaccggc gacatgcgca ggaagaaatc gccaaaatgc cagccagccg ctttagcgat
     3541 gatcaggttc tgtggttcgc ccaccatggt cattacgccg cctaatgcgg tgccgacacc
     3601 ggcatgcatc atcaggctac gcagaaagcc acggaactgt tccagaacca ctttgtaatg
     3661 cttgtcgata tgactatcgt cttgcaggtc ggtgtcttcg gtacgggaag aggctacgcg
     3721 atgataaata ccataaaaac cgacggcaac gctgatcacc accgccacga cggttaaggc
     3781 atcgaggaac gcggagagga acgctgccgc tacgcaaaaa gagagcgaca gcagcatttt
     3841 ggagcgaatg cttaacagca aacgggtaaa tatgaacagc aatagctgtt tcataaaata
     3901 gatacccgcc accataaaca tcagtaacag caagacttca agatttgccg ccacctcttc
     3961 acggacgtgt tccgcgctgg tcatgccgat gaataccgct tcgatagcca acaggccacc
     4021 ggggagcagc gggtagcatt tcagggccat cgccagagtg aaaataaatt ccgctaccag
     4081 caaccagcca gcgacgaaag ggctgatgag gaaaattaac ggatttacga ttaagaaaat
     4141 gatgagggcg agtttgtacc agtcggggga ctggcccaaa aaattgcgcc atagcgcgcg
     4201 gccccaggag atctccatga tggtttccct taccttacaa ataatcaatg atgtttttat
     4261 gtttaaacgc aaagcttaac ggtcaggcag gagtgaggca agtcttgata gtcaagggga
     4321 aagagatgcg gaaaatgaag ccttgatccc ttttttcttc tttttgtctg ctatcagcgt
     4381 agttagccct ctggtatgat gagtccaact ttgttttgct gtgttatgga aatctcacta
     4441 tggtcattaa ggcgcaaagc ccggcgggtt tcgcggaaga gtacattatt gaaagtatct
     4501 ggaataaccg cttccctccc gggactattt tgcccgcaga acgtgaactt tcagaattaa
     4561 ttggcgtaac gcgtactacg ttacgtgaag tgttacagcg tctggcacga gatggctggt
     4621 tgaccattca acatggcaag ccgacgaagg tgaataattt ctgggaaacc tctggtttaa
     4681 atatccttga aacactggcg cgactggatc acgaaagtgt gccgcagctt attgataatt
     4741 tgctgtcggt gcgtaccaat atttcaacta tttttattcg caccgcgttt cgtcagcatc
     4801 ccgataaagc gcaggaagtg ctggctaccg ctaatgaagt ggccgatcac gccgatgcct
     4861 ttgccgagct ggattacaac atattccgcg gcctggcgtt tgcttccggc aacccgattt
     4921 acggtctgat ccttaacggg atgaagggac tgtatacgcg cattggtcgt cactatttcg
     4981 ccaatccgga agcgcgcagt ctggcgctgg gcttctacca caaactgtcg gcgttgtgca
     5041 gtgaaggcgc gcacgatcag gtgtacgaaa cagtgcgtcg ctatgggcat gagagtggcg
     5101 agatttggca ccggatgcag aaaaatctgc cgggtgattt agccattcag gggcgataat
     5161 cccttccgtt taaaaaacaa acccctcaaa cgaggggttt tttgttgttt ttacagattt
     5221 cccattctcg gcgggcaacg ttccagcaac tcgacgctgc cgtcttcgtt ttgctgttcc
     5281 agcatcacat caaatcccca caggcgatgc acatgcttca gtacttcttt gcgcccccga
     5341 tccagcggtg cgcgattatg tggaatgtaa cgcagcgtca gcgaacggtc gccgcgcaaa
     5401 tccacgttcc agatctgaat attcggctcc agattactta agttatattg cgacgatagc
     5461 cggttacgga tctcccgata accttcttca ttatgaatag cggaaatctc cagataatta
     5521 tgccgatcat catccagcac ggtgaagaag cggaaatcac gcatcacttt cggcgacagg
     5581 aactggctga taaaactctc atctttgaaa tcacgcatcg cgaaatgcag cgtttccagc
     5641 cagtcggaac cggcgatatc cgggaaccag tatttgtctt cttccgttgg cgactgacaa
     5701 atccgtttaa tatcctggaa catggcgaac ccgagggcat acgggttgat gccgctgtac
     5761 cacgggctgt tatagggggg ctggaagacc acattggtgt ggctgtgcaa aaactccagc
     5821 ataaaacgtt ccgttacttt cccttcatca tacagatggt taaggatggt gtagtgccag
     5881 aaggtggccc agccttcgtt catcacctga gtctgttttt gcggataaaa atactggctc
     5941 accttacgca caatacgcag gatttcacgc tgccatgatt ccagcagtgg cgcatttttc
     6001 tccataaaat agagcaggtt ttcttgtggt tcggacggat aacggcgcgc ttcagcaacc
     6061 gttttctctt cctcgcgctt cggcagggta cgccagagca tattgacctg actttgcaaa
     6121 tactcttcgc gacttttctg ccgggctttc tcttcttgca gcgagatttt ttgcgggcgt
     6181 ttgtaccggt ccacgccgta gttcatcagc gcgtggcacg agtccagaag ccgttctact
     6241 tcatcaacgc cataacgttc ttcgcactcg gtaatatatt tacgggcaaa aatcagataa
     6301 tcgacaatcg aactggcgtc ggtccagcta cggaataagt aattgttttt gaagaaagag
     6361 ttatgcccat agcaggcatg agccatcacc agcgcttgca tggtaatggt gttctcttcc
     6421 atcaggtaag cgatacacgg gttagagtta atgacgattt cataggccag tccttgctga
     6481 ccgtgcttat acagccgttc agtctcgata aactttttac cgaatgacca gtgcggatag
     6541 ttaattggca tgccgacgct ggagtaggca tccatcatct gttctgaggt tatcacttca
     6601 atctggtgcg ggtaggtatc cagccggtag agtttcgcca cccggtctat ctctgccaga
     6661 taaacatcca gcaggtcgaa cgtccagtcg ggtccatcgc tcaaacgtgt ggtgtcctta
     6721 ttcatagaat cgatcgtcgc catacgcgca cctcattgtt gtcggcgctc tctgtgtgga
     6781 gcacctcatt gttgtcggcg ctctctgtgt ggagcacctc atttcaagca tagaacacct
     6841 gttaaaaacc gcgtcgccgg agaatttttt tctttgcgat ttcttattat cagagtgcca
     6901 ctaatccgtt tctgaacgga attttatgct ggataaaaag ggcgttcagc aggagatact
     6961 aaagacgcca tattgccgca gagtcaggga gatgtgagcc agctcaccat aaaaaagccg
     7021 tatgttgaat aatattttca actgagttat caagatgtaa ttagattatt attcttttac
     7081 tgtatctacc gttatcggag tggctatgcg agttgtcata ctgggaagtg gtgtggtagg
     7141 cgttgccagc gcctggtact taaatcaggc aggacatgag gtcaccgtca ttgatcggga
     7201 gccgggggcc gccctggaaa ccagtgccgc gaatgccggg caaatctccc ccggatatgc
     7261 tgcaccgtgg gcggcaccag gtgtgccttt aaaagcgatt aaatggatgt tccagcgcca
     7321 tgcgccgctg gcggttcgtc tcgacggtac gcagttccag ttgaaatgga tgtggcaaat
     7381 gttacgtaac tgcgacacca gccactacat ggaaaacaaa gggcggatgg tgcgtctggc
     7441 ggaatacagc cgtgattgcc tgaaagcatt gcgcgccgaa accaatattc agtatgaagg
     7501 gcgtcagggt gggacgctgc aactgttccg taccgaacaa cagtatgaaa atgcgacccg
     7561 cgatatcgcc gtgctggaag atgccggcgt accgtatcag ctgctggaat ccagccgcct
     7621 ggcggaagtg gagcccgcgc tggcagaagt agcgcacaaa ctgacgggtg gtctgcaatt
     7681 gcccaatgat gaaaccggag actgccagct gtttacccag aatctggcgc ggatggcgga
     7741 gcaggcgggg gttaaattcc gctttaatac gcccgttgac caactgcttt gcgacggcga
     7801 gcaaatctac ggcgtgaagt gtggcgatga agtgattaag gccgatgcgt atgtgatggc
     7861 gtttggttct tactcgacgg cgatgctcaa aggcattgtt gatattccgg tgtatccgct
     7921 gaaaggctac tcgctgacca ttccaattgc gcaggaagat ggtgcgccgg tatccaccat
     7981 tcttgatgaa acctacaaaa tcgccattac ccgtttcgat aaccgcattc gtgttggcgg
     8041 aatggcggaa attgttggtt ttaataccga gctgttgcaa ccgcgtcgtg aaacgctgga
     8101 gatggtggtt cgcgatctct atccacgcgg cggtcatgtc gagcaggcga ctttctggac
     8161 gggtctgcgc ccgatgacgc cagacggcac gccggttgtc gggcgtacac gctttaaaaa
     8221 tctgtggcta aataccggtc acggcacgct cggctggacg atggcttgcg gttccggtca
     8281 gttgttaagc gatctgctct ctggtcgcac gccagcgatc ccatatgagg atctaagcgt
     8341 agcgcgctac agccgtggat ttacgccatc acgtccgggc catttacatg gcgcacacag
     8401 ctaaggaaac gagatgaccc gtccgataca ggccagcctc gatctgcagg cattaaaaca
     8461 gaatctgtcc attgtccgcc aggccgcgcc gcacgcgcgc gtctggtcgg tggtaaaagc
     8521 gaacgcttac gggcacggta ttgagcgtat ctggagcgcg ctcggggcta ccgatggctt
     8581 tgcattactt aacctggaag aagcaataac gttacgtgag cgcggctgga aaggaccgat
     8641 cctgatgctg gaaggatttt tccatgctca ggatctggaa atgtatgacc agcaccgcct
     8701 gaccacctgc gtacacagca actggcagct caaagcactg caaaatgcgc ggctaaaagc
     8761 accgttggat atttatctta aagtgaacag tgggatgaat cggttgggct tccagcccga
     8821 tcgcgtgctt accgtctggc agcagttgcg ggcgatggcg aatgttggcg aaatgaccct
     8881 gatgtcgcat tttgccgagg cggaacatcc tgatggaatt tccggcgcga tggcgcgtat
     8941 tgagcaggcg gcggaagggc tggagtgtcg gcgttcgttg tccaattcgg cggcgactct
     9001 gtggcaccca gaagcgcatt ttgactgggt tcggcctggc attattttgt atggcgcttc
     9061 gccgtccggt cagtggcgtg atatcgccaa tactggatta cgtccggtga tgacgctaag
     9121 cagtgagatt attggtgtcc agacgctaaa agcgggcgag cgtgtgggct acggcggtcg
     9181 ttatactgcg cgcgatgagc agcgaatcgg cattgttgcc gcaggatacg ccgacggtta
     9241 tccgcgccac gcgcctaccg gtacccctgt tttagtggac ggcgtgcgca ccatgacggt
     9301 ggggacagtc tcgatggata tgctggcggt cgatttaacg ccttgcccgc aggcgggtat
     9361 tggtacgcca gttgagctgt ggggcaagga gatcaaaatt gatgatgtcg ccgccgctgc
     9421 cggaacggtg ggctatgagt tgatgtgcgc gctggcgtta cgcgtcccgg ttgtgacggt
     9481 gtaacttgtt gtaagtcgga tcggaggcaa cgtcttctgg ttgcaaaaaa aatcatccat
     9541 ccggctggtc agcaactgta gttgttaatg tgacagagcc attgcccatg atagtgccca
     9601 ttaaaaggat ggacactatt tccccggaac ctgaactcac cgcacaggcg ttctacataa
     9661 aacgcttacg cttcattgtt gactcgaact cgacttcaga taaatcacgc ttcacccttg
     9721 atgcagattc gggttcatgt tcagaatgtt gattaacagg ctgcg
//
LOCUS       DAZFAB010000143         9707 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZFAB010000143 DAZFAB010000000
VERSION     DAZFAB010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9707
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.143"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            27..2057
                     /gene="malS"
                     /locus_tag="ACHJWA_004235"
     CDS             27..2057
                     /gene="malS"
                     /locus_tag="ACHJWA_004235"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO4310717.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDASAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKAIDGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCIAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            2235..3488
                     /gene="avtA"
                     /locus_tag="ACHJWA_004236"
     CDS             2235..3488
                     /gene="avtA"
                     /locus_tag="ACHJWA_004236"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFO4310718.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEAQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(3640..4113)
                     /gene="ysaA"
                     /locus_tag="ACHJWA_004237"
     CDS             complement(3640..4113)
                     /gene="ysaA"
                     /locus_tag="ACHJWA_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFO4310719.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(4215..4355)
                     /locus_tag="ACHJWA_004238"
                     /pseudo
     CDS             complement(4215..4355)
                     /locus_tag="ACHJWA_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
     gene            complement(4357..5172)
                     /locus_tag="ACHJWA_004239"
     CDS             complement(4357..5172)
                     /locus_tag="ACHJWA_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4310720.1"
                     /translation="MLELSMTLPIKVQNGGLFISRGVGRHSARRLESWEIIFVEKGRL
                     TIQEEERIFLVDAGESLLLWPNRQHVGVEDFPADLKFYWLHFEVKAPDNDPRWLTHLS
                     VPQQTKVVDPQALVALFRQFLSEQEKHQRTPALEFIVLLILQQLTRDARLDEQADGAG
                     TTLAWKAQKLIRTQYHRPLSTALLARELYCNADYLGRVYRRVFHLTLTEAIHRQRVRE
                     AEKLLISEARSLTEVAALCGFNDVGYFRQIFRKHTGLTPAAWKRRYSKEHINS"
     gene            5398..6798
                     /locus_tag="ACHJWA_004240"
     CDS             5398..6798
                     /locus_tag="ACHJWA_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4310721.1"
                     /translation="MTSTPITTADIAKGKIDDALSVREKIGYGLGDAGGTVITCLIMN
                     FLTFFYTDVFGLTPALVGTLFIALRVFDAISDPVMGVIADRTQSRWGRFRPWQLWIAI
                     PIGIIGILTFTVPDASMGVKIAWAFGTYLLLSVGYTAINVPYCARINTMTTRHNEVIS
                     CQSWRFVLCGVAGFLVSVGLPWMVALFGQGNAARGYQLGVGVLCAIAVVMFLCCFFWV
                     RERVPLSTMGKFTLREHLAGLRNNDQLLLMLVMSFLLINVFNIRGGGYMYFITYVLQG
                     STGYTSLFFTMVTFASIIGSVIVSPLTRRFDTVKIYYYTNLLLAALAVLMWFLPSGPA
                     YQTLWLAVILGNGVILGFTLPLHFSLMAFADDYGEWKTHVRSSGMNFAFNLFFIKLAW
                     ASSAGIISLLFIFVAYQPGVENQTASSLGGIAAMETLLPALFHLLLAGAIRFCKLNNP
                     MMSRIATDLRQRHVQP"
     gene            6809..8779
                     /locus_tag="ACHJWA_004241"
     CDS             6809..8779
                     /locus_tag="ACHJWA_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HFO4310722.1"
                     /translation="MNISEVDLHKLTVSDPFLGQYQQLVRDVVIPYQWDALNDRIPEA
                     EPSHAIENFRIAAGLQEGEFYGMVFQDSDVAKWLEAVAWSLCQKPDAELEKTADEVIE
                     LIASAQCEDGYLNTYFTVKAPEERWSNLAECHELYCAGHLIEAGVAFFQATGKRRLLG
                     VVCRLADHIDSVFGPDESKLHGYPGHPEIELALMRLYEVTEEPRYLALTNYFVEQRGA
                     QPHYYDQEYEKRGQTSHWHTYGPAWMVKDKAYSQAHLPLAQQQTAIGHAVRFVYLMTG
                     VAHLARLSHDDSKRQDCLRLWNNMAQRQLYITGGIGSQSSGEAFSSDYDLPNDTVYAE
                     SCASIGLMMFARRMLEMEGDSQYADVMERALYNTVLGGMALDGKHFFYVNPLEVHPKS
                     LKFNHIYDHVKPIRQRWFGCACCPPNIARVLTSIGHYLYTPREDALYINIYAGNSMEV
                     PVENGTLRLRVSGNYPWQEQVTIAVESPQPVRHTLALRLPDWCTQPQIILNGEEVEQD
                     IRKGYLHITREWQEGDTLNLTLPMPVRRVYGNPLVRHVAGKVAIQRGPLVYCLEKADN
                     GESLHNLWLPTDAPFTTFEGKGLFSHKILIQAPGYRYEQSNPEQQPLWHYDSAPAKRQ
                     TQTLTFIPWFSWANRGEGEMRIWVNEEKHCHP"
     gene            complement(8909..9649)
                     /gene="yiaT"
                     /locus_tag="ACHJWA_004242"
     CDS             complement(8909..9649)
                     /gene="yiaT"
                     /locus_tag="ACHJWA_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HFO4310723.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDSFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDYAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITDSPMIDRNESFTFVTGVSWRF"
BASE COUNT         2350 a         2611 c         2468 g         2278 t
ORIGIN      
        1 atcgcagcaa taaggactca tccaccatga aactcgccgc ctgttttctg acactccttc
       61 ctggcttcgc cgttgccgcc agctggactt ctccggggtt ccctgccttt agcgaacagg
      121 gaacgggaac atttgtcagc cacgcgcagt tgcccaaagg tacgcgtcca ctcacgctaa
      181 attttgacca gcagtgctgg cagcctgcag atgcgataaa actcaatcag atgctttccc
      241 tgcaaccttg tagcaacacg ccgcctcaat ggcgattgtt cagggacggc aaatatacgc
      301 tgcaaataga cacccgctcc ggtacgccaa cattgatgat ttccatccag aacgccgccg
      361 aaccggtagc aaacctggtc cgtgaatgcc cgaaatggga tggattaccg ctcacgctgg
      421 atgtcagcgc cactttcccg gaaggagccg ccgtacggga ttattacagc cagcaaattg
      481 cgatagtgaa gaacggtcaa ataacgttac aacccgctgc taccagcaac ggtttactcc
      541 tgctggaacg ggcagaaact gacgcctctg cccctttcga ctggcataac gccacggttt
      601 actttgtgct gacagatcgt ttcgaaaacg gcgatcccag taatgaccag agttacggac
      661 gtcataaaga cggtatggcg gaaattggca cttttcacgg cggcgattta cgcggcctga
      721 ccaacaaact ggattacctc cagcagttgg gcgttaatgc tttatggata agcgccccat
      781 ttgagcaaat tcacggctgg gtcggcggcg gtacaaaagg cgatttcccg cattatgcct
      841 accacggtta ttacacacag gactggacga atcttgatgc caatatgggc aacgaagccg
      901 atctacggac gctggttgat agcgcacatc agcgcggtat tcgtattctc tttgatgtcg
      961 tgatgaacca caccggctat gccacgctgg cggatatgca ggagtatcag tttggcgcgt
     1021 tatatctttc tggtgacgaa gtgaaaaaaa cgctgggtga acgctggagc gactggaaac
     1081 ctgccgccgg gcaaacctgg catagcttta acgattacat taatttcagc gacaaaacag
     1141 gctgggataa atggtgggga aaaaactgga tccgtaccga tatcggcgat tacgacaatc
     1201 ctggattcga cgatctcacc atgtcgctag cctttttgcc ggatatcaaa accgaatcaa
     1261 ctaccgcttc tggtctgccg gtgttctata aaaacaaaac ggatacccac gctaaagcca
     1321 tcgacggctt tacccctcgc gattacttaa cccactggtt aagtcagtgg gtccgcgact
     1381 atgggattga tggttttcgg gtcgataccg ccaaacatgt tgagttgccc gcttggcagc
     1441 aactgaaaac cgaagccagc gccgcgcttc gcgaatggaa aaaagctaac cccgacaaag
     1501 cattagatga caaacctttc tggatgaccg gtgaagcctg gggccacggc gtgatgcaaa
     1561 gtgactacta tcgccacggc ttcgatgcga tgatcaattt cgattatcag gagcaggcgg
     1621 cgaaagctgt cgattgtatt gcgcagatgg atacgacctg gcagcaaatg gcggagaaat
     1681 tgcagggttt caacgtgttg agctacctct cgtcgcatga tacccgtctg ttccgtgaag
     1741 ggggcgacaa agcagcagag ttattactat tagcgccagg cgcggtacaa atcttttatg
     1801 gcgatgaatc ctcgcgtccg ttcggtccta caggttctga tccgctgcaa ggtacacgtt
     1861 cggatatgaa ctggcaggat gttagcggta aatctgccgc caacgtcgcg cactggcaga
     1921 aaatcagcca gttccgcgcc cgccatcccg caattggcgc gggcaaacaa acgacacttt
     1981 cgctgaagca gggctacggc tttgttcgtg agcatggcga cgataaagtg ctggtcatct
     2041 gggctgggca acagtgactt ttccggcttc ccgttcgtca gtacctcggg aagccgccaa
     2101 ccaggataaa atgtcagccc taatcagcgt tgcaggataa agcaccgctc actcttcaac
     2161 agaccgattt gcaccccagc aaatgtagcg ttattgttac cttccttgct acagagttcg
     2221 acagatatcc cgctatgaca ttctcccttt ttggtgacaa atttacccgc cactccggca
     2281 ttacgctgct gatggaagat ctgaacgacg gtttacgcac gcctggcgcg attatgctcg
     2341 gcggcggtaa tccggcgcag atcccggaaa tgcaggacta cttccagacg ctactgaccg
     2401 acatgctgga aagtggcaaa gcgactgatg cactgtgcaa ttacgacggt ccacagggga
     2461 aaacggagct actcacactg cttgccggaa tgctgcgcga gaagttgggt tgggatatcg
     2521 aagcacagaa tattgcacta acaaacggca gccagagcgc gtttttctac ttatttaatc
     2581 tgtttgccgg acgccgtgcc gatggtcggg tcaaaaaagt gctgttcccg ctggcaccgg
     2641 aatacattgg ctacgctgac gccggactgg aagaagatct gtttgtctct gcgcgtccga
     2701 atattgaact gctgccggaa ggccagttta aataccacgt cgattttgag catctgcata
     2761 ttggcgaaga aaccgggatg atttgcgtct cccggccgac gaatccaaca ggcaatgtga
     2821 ttactgacga agagttgctg aagcttgacg cgctggcgaa tcaacacggc attccgctgg
     2881 tgattgataa cgcttatggc gtcccgttcc cgggtatcat cttcagtgaa gcgcgcccgc
     2941 tatggaatcc gaatatcgtg ctgtgcatga gtctttccaa gctggggctg cctggctccc
     3001 gctgcggcat tatcatcgcc aatgaaaaaa tcatcaccgc catcaccaat atgaacggca
     3061 ttatcagcct ggcacctggc ggtattggtc cggcgatgat gtgtgaaatg attaagcgta
     3121 acgatctgct gcgcctgtct gaaacagtca tcaaaccgtt ttactaccag cgtgttcagg
     3181 aaactatcgc catcattcgc cgctatttac cggaagatcg ctgcctaatt cataaaccag
     3241 aaggagccat tttcctctgg ctttggttta aggatttgcc cattacgacc gagcagctct
     3301 atcagcgcct gaaagcacgc ggcgtgctga tggtgccggg gcacaacttc ttcccagggc
     3361 tggacaaacc gtggccgcat acgcatcaat gtatgcgcat gaactacgta ccagagccgg
     3421 agaaaattga ggcgggggtg aagattctgg cggaagagat agaaagagcc tgggctgaaa
     3481 gtcactaaaa attgcctgat gcgctacgct tatcaggcct acatgatttc tgcaatatat
     3541 ttaatttgca agaatttgtg ggccggataa ggcgtttacg ccgcatctgg cataaacaaa
     3601 gcgcactatt ccggcgttga gaaacgccgg aaaaacgttt caaacaggct gctgccgtag
     3661 ccgatgccgc tgcactttct cgacatccat gcactgcaac gcctttgtcg ggcaggcttc
     3721 aacacaggcc ggtcccgtct cccgatgcca gcacagatcg cacttaatcg ccctcgcctt
     3781 tttgcgcgaa gaaacgacct ccatcgcacc aaacgggcaa gccagcatac agcttttaca
     3841 gccaatgcaa cgtgtttgtt caacgaaaat atgcccatgt tcgcggctta tcgcgtcaac
     3901 agggcagaca ttcgcgcacg gtgcatcttc acactgatga caggctactg ccgtggtcca
     3961 gctgtggtct ttaatgacac gaatacggga aataaactcg tctggtgaca acgcagcgca
     4021 atcctgattc tcctgatgcg acactgcgca agccacttca caggtacggc aaccgataca
     4081 tttcgtcgca tccgcaataa taaaccggtt catgatcact cctttgcact aaaacagcag
     4141 agtgccagag agcgcgtccc tactgctttg atccaacgaa ggaaatgtta aagttttggc
     4201 acttgtccaa agcgttatgt gatcgcgccc tggtcgtccc tgacagtaaa tcccagttca
     4261 ttagaaatag cctgcgcggt ttcacgcagt ggtttcagga gatttttctc ccccacctgt
     4321 ttcagacgtg atgtcgaaag cgaaatcgcc ccggcactac gaattgatat gttccttgct
     4381 gtaacgccgc ttccacgctg ctggcgttaa accggtgtgt ttgcgaaaaa tctgccggaa
     4441 atagccaacg tcattaaacc cgcacaacgc cgccacttcc gtgagcgatc gcgcttcgct
     4501 aattaacagc ttttcggctt cgcgcacccg ctggcgatgg attgcttctg ttaacgtcag
     4561 atgaaacacc cgccggtaga cgcgcccaag ataatcggca ttgcagtaca gctcgcgggc
     4621 cagaagcgcc gtagaaagcg ggcgatgata ttgcgtgcgg atcagtttct gcgctttcca
     4681 ggcaagggtg gtgcctgcgc catcggcctg ttcatcaaga cgcgcgtcac gggtgagctg
     4741 ctgcaaaatg agcagcacaa taaactccag cgccggagtg cgttgatgct tttcctgttc
     4801 gcttaagaac tggcgaaaca gggccaccag cgcctgcgga tcgacaacct tcgtttgttg
     4861 cggcacgcta agatgagtaa gccagcgagg gtcgttgtcc ggcgctttaa cctcaaaatg
     4921 cagccagtaa aatttgagat cggcgggaaa atcctccacc ccaacatgtt gtcggttcgg
     4981 ccagagtaat aaactctctc ccgcatcgac cagaaatatc cgttcttctt cctgaatcgt
     5041 taatcgacct ttttcgacaa agataatttc ccatgactcc agccgccttg cagaatgcct
     5101 ccctacgcca cgggaaataa ataatccacc attttgcact ttaatcggta acgtcatgga
     5161 taattcgagc attgatatgt aatttcccgc ttattcatta atgattaaac acattacctt
     5221 ttgattatta atcaaaaaca ttaatttcac ctgatccatt tcacactttt aaattcaggt
     5281 cggaatcgtc accttttttt actcttttct cgccttgttg atattcccgt tcacggcaga
     5341 attaatcagg tcatttcaat aaaactaaaa attgctccct attcacgagg aatatctatg
     5401 acctctacac cgattactac cgctgatatc gctaaaggta aaattgacga tgcgttatct
     5461 gtacgggaaa aaataggcta cggcctgggt gacgcaggcg gcaccgtaat aacttgcctg
     5521 atcatgaatt ttctcacctt tttctacacc gacgtttttg gattaactcc ggcgctggtt
     5581 ggcacgctgt ttattgcact gcgcgtgttt gatgccatct ccgacccggt gatgggcgtc
     5641 attgccgacc ggacgcaaag ccgatggggg cgctttcgtc cgtggcagct atggattgcc
     5701 attcccatcg gcattatcgg catcctgacg ttcaccgtgc cagatgccag catgggagta
     5761 aaaatcgcct gggcgttcgg tacttacctg ctcctttcag tcggttatac cgccatcaac
     5821 gtaccgtact gcgcgcggat caacaccatg accacccgcc acaatgaagt gatctcctgc
     5881 cagtcctggc gattcgttct ctgcggcgta gcgggatttc tggtttcggt aggcttaccg
     5941 tggatggtag ctctcttcgg tcagggcaac gctgcacgcg gctatcaact gggcgtcggg
     6001 gtattgtgcg ccattgccgt ggtgatgttc ctgtgctgtt tcttctgggt tcgtgaacgg
     6061 gtgccgctct ccacaatggg gaaatttacc ctgcgcgaac atcttgccgg gctgcggaac
     6121 aacgaccaac tgctgctgat gctggtcatg tctttcctgc tgattaacgt ctttaacatt
     6181 cgcggcggtg ggtatatgta cttcattacc tacgtcttac aaggcagcac gggctacacg
     6241 tcgctgttct tcaccatggt caccttcgcc tccattatcg gctcggtgat tgtcagcccg
     6301 ttaacgcggc gtttcgatac cgtcaaaatt tattactaca ccaacctgct cctcgctgca
     6361 ctggcggtgt tgatgtggtt cctgccctcc ggcccggctt atcaaacgct gtggctggcg
     6421 gtgatcctcg gtaatggcgt gattcttggc ttcacattgc cactgcactt ctcattgatg
     6481 gcctttgccg atgactacgg cgagtggaaa acccacgtac gttcttccgg catgaacttc
     6541 gccttcaatc tgtttttcat caagctggcc tgggcctcca gcgccgggat catcagcctg
     6601 ctgtttattt ttgtcgccta ccagcctggc gtggaaaacc agaccgccag ttcgcttggc
     6661 gggatcgcgg cgatggaaac attactgcct gcgctattcc acctgctgct ggcaggggcg
     6721 atccgctttt gcaaactcaa taatcctatg atgtcacgca tagctaccga cctgcgtcag
     6781 cgtcatgtac agccttaagg agcacaagat gaacatttcg gaagtcgatc tgcataaact
     6841 gacggtcagc gatccgttcc tcggtcagta ccaacaactg gtccgcgacg tggtgattcc
     6901 ttatcagtgg gatgccttga acgatcgtat cccagaagcg gaacccagcc atgcgattga
     6961 aaactttcgc attgccgccg gacttcagga gggtgaattt tacgggatgg tgtttcagga
     7021 cagcgacgtc gccaaatggc tggaagcggt agcctggtcg ctgtgccaga agccggacgc
     7081 cgaactggaa aaaaccgccg acgaggtaat cgaactgatc gcctccgccc aatgcgaaga
     7141 cggctatctc aatacttact ttacggtaaa agcacccgaa gaacgctgga gcaatctggc
     7201 ggagtgtcat gaactttact gcgcaggtca tctgattgaa gccggagtcg ccttcttcca
     7261 ggccacggga aaacggcgct tgctgggggt cgtttgccgc ctggccgatc atatcgacag
     7321 cgtatttggt ccagatgaaa gtaagttaca cggttatcct ggtcacccgg aaattgaact
     7381 ggcactaatg cgcctgtatg aagtgactga agagccgcgc tacctggcgc tgacgaacta
     7441 ttttgtcgaa cagcgtggtg cgcaaccgca ctattacgac caggaatatg aaaagcgcgg
     7501 gcagacatcg cactggcaca cctacggccc ggcgtggatg gtgaaagaca aagcctacag
     7561 ccaggcacat ttgccccttg cgcaacagca aaccgccatc ggtcacgcgg tacgttttgt
     7621 ctatctgatg accggcgtcg cgcatctcgc gcgtttaagt cacgatgaca gcaagcgtca
     7681 ggactgcctg cggctgtgga acaatatggc ccagcgtcag ttatatatta ccggcggcat
     7741 cggctcacaa agcagcggcg aagcgttcag cagcgattac gatctgccga atgacacggt
     7801 ttacgccgaa agttgtgctt ccatcggcct gatgatgttc gcccgacgaa tgctggaaat
     7861 ggaaggcgac agtcaatatg ccgatgtgat ggagcgcgca ctgtacaaca ctgtgctcgg
     7921 cggcatggca ttggatggca aacatttctt ctatgtgaat ccactggaag tacatccaaa
     7981 atcgctgaaa ttcaaccata tctacgatca cgttaaaccg atccgccagc gttggtttgg
     8041 ctgcgcttgt tgtccgccaa atatcgcccg cgtgctgacc tcgattggtc attatctcta
     8101 cacgccgcgt gaagatgcgt tgtatatcaa catctacgca ggaaacagca tggaagtgcc
     8161 ggtagaaaat ggcacgctgc gcctgcgggt tagcgggaac tatccgtggc aggagcaggt
     8221 gacgattgcg gttgaatcgc cccagccggt acgtcatacg ctggctttac gtctgccgga
     8281 ctggtgcaca cagccgcaga tcatattgaa tggggaagag gtcgagcagg atattcgtaa
     8341 agggtatttg cacattaccc gcgaatggca ggagggcgat acgctgaatc tgactttgcc
     8401 gatgccggta cgccgcgttt acggtaaccc gctggtgcgt cacgtcgccg gaaaagtggc
     8461 gattcagcgc ggcccgctgg tgtattgcct ggaaaaggcc gacaacggcg agtcactgca
     8521 taatctgtgg ctgcccaccg atgcgccatt tacgacattt gaaggcaagg gattgtttag
     8581 ccataagatc ttaatccagg caccgggtta ccggtatgaa cagagcaatc cagagcagca
     8641 accgctgtgg cattacgaca gcgcgccagc caaacgccag acgcaaactc tgacctttat
     8701 cccgtggttt agctgggcca accggggtga aggcgaaatg cggatctggg tgaatgagga
     8761 aaagcattgc catccgtagg ttggataagg cactcgcgcc gcatccaacg taaggagcac
     8821 attgcctgat gcgacgctta acacgtctta tcaggcatac aagtcggtgc gagagcgtag
     8881 gccagccgca ccgacatcca ccggtgaatt aaaaacgcca gctcacgccg gtgacaaagg
     8941 taaaactctc attacgatca atcatcgggc tgtcggtaat ctcttccggc agctcgctgt
     9001 atcctgcgct cgccatcaat acgacgtgct ctcctatcgg gtattttgcc gtcaggctga
     9061 catagggcac ccaggcatcc tgcgcggaat aacttgccag accgctacga cgggactcac
     9121 tctctgaaat gccatagtaa tagtcactga aattctcgtc ataatagaga acgcccagcg
     9181 caggtgtcag cgacagacga cctatctgca ttttgtggaa taccgatagc tcccccaccc
     9241 agccgttgct gttatccaga acgtccgcag ctgcagaggc tttcacggtt ccccaacgct
     9301 cgtggtgata ccaggcaacc cccgccatag ccgtactatc acgcttatca agctgttgca
     9361 tggcataatc gtcattatcg gcagggtcaa attccagcgg catccaggat gcggtcaggc
     9421 taagttcatt tttttcactt tgcgacagaa taaaacctaa cgtggtctga cgaacataaa
     9481 aagaatcacc ttcataacta atcagcggaa ttgcctttgt attttcatta taaccgcgat
     9541 aaggcgattc attataagcc gcaccagcac caatggataa ttcagaagca gttgcgcttg
     9601 ccataaaagg caacgcaaat aacgccacaa tattgcggtt aattaacatg tccggtattc
     9661 cattttaaaa taagataaaa aagagtcggc atagtaatgc ttacaat
//
LOCUS       DAZFAB010000144         9467 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZFAB010000144 DAZFAB010000000
VERSION     DAZFAB010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9467
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.144"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            372..2273
                     /locus_tag="ACHJWA_004243"
     CDS             372..2273
                     /locus_tag="ACHJWA_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFO4310724.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLTQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTILQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MEGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     RGAGTALENHFAVIPADRTWRPQPRLKPLVDGPQSAVVTGPEGEEIFCDEHGRVRVKF
                     NWDRYNPADQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENHTPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGREQVYIHAQKNMDTEVLND
                     RTTTVKHDHRETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGS
                     VHVDAVNNGGEGNGIQAYTAIKEIMLAVEESKIALTPDGIQLQVGESTVIRLSKDGIT
                     IVGGSVFIN"
     gene            2301..2762
                     /locus_tag="ACHJWA_004244"
     CDS             2301..2762
                     /locus_tag="ACHJWA_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFO4310725.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREPVSQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            2782..7632
                     /locus_tag="ACHJWA_004245"
     CDS             2782..7632
                     /locus_tag="ACHJWA_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO4310726.1"
                     /translation="MSEGPGGPQGATAGGTLAMRMLSQRAMAASQMKRAANDKAIAQM
                     LASKKSGPPAARLGDEIQHKSFLGALAGAVLGAIVTIAEGCLIMAACATGPYALVLVP
                     ALMYASYKASDYVEEKQNQLESWINSFCDTDGAINTGSKNVKINGKPAARAAVTLPPP
                     PPPGAIPEVPQGEPSWGDIATDLLESAAEKAVPLAKAWGNAVITLTDSNAGFMDRVSA
                     GASLLFPAGPVLMEFATMVGGRGEIKKDVDFPEAGEDTALCDKENKPPRIAQGSSNVF
                     INNQPAARKGDKLECSAAIVEGSPDVFIGGEQVTYLDIQPEFPPWQRMILGGITIASY
                     LLPPAGLLGKLGNLAKLGKLGNLLGKSGKLLGAKLGALLGKTGNSLKSIANKVIRWVT
                     DPVDPVTGAYCDERTDFTLGQTLPLSFTRFHSSVLPLHGLTGVGWSDSWSEYAWVREQ
                     GNRVDVISLGATLNFAFDGESDTAVNPYHAQYILRRRDDYLELFDRDALSSRFFYDAF
                     PGMRLRHPVTDDTSDDRLAHSPADRMYMLGGMSDTASNRITFERDTQYRITGVSHTDG
                     IRLKLTYHASGYLKAIHRTDNGIQTLATYEQDARLDYHLFYEYDAADRIIRWSDNDQT
                     WSRFTYDAQGRCVTVTGAEGYYNATLDYGDGCTTVTDGKGIHRYYYDPDGNILREEAP
                     DGSTTTYEWDEFHHLLARHSPAGRVEKFEYNAAHGQLSRYTAADGADWQYCYDERGLL
                     SNITAPAGQTWTQQCDERGLPVSLVSPQGEETRLAYTPQGLLSGIFRQDERRLGIEYD
                     HHNWPETLTDVMGREHHTEYSGHDLPVKMRGPGGQSVRLQWQQHHKLSGLERAGTGAE
                     GFRYDRHGNLLAYTDGNGVVWTMEYGPFDLPVARTDGEGHRWQYRYDKDTLQLTEVIN
                     PQGESYLYILDNCGRVTEERDWGGVVCRYRYDADGLCTARVNGLEETILYSRDAAGRL
                     AEVITPEGKTQYAYDKSGRLTGIFSPDGTSQRTGYDERGRVNVTTQGRRAIEYHYPDE
                     HTVIRCILPPEDERDRHPDGSLLKTTYRYNAAGELTEVILPGDETLTFSRDEAGREVL
                     RHSNRGFACEQGWNAAGQPVSQRAGLFPAEATWGGLLPSLLREYRYDSAGNVSGVTSR
                     EDYGRETHREYRLDRNGQVTAVTASGTGLGYGEGDETYGYDSCGYLKAQSAGRHRISG
                     ETDQYAAGHRLKQAGNTQYDYDAAGRMVSRTKHRDGYRPETERFRWDSRDQLTGYRSA
                     QGEQWEYRHDASGRRTEKRCDRKKIRFTYLWDGDSIAEIREYRDDKLYSVRHLVFNGF
                     ELISQQFSRVRQPHPSVAPQWVTRTNHAVSDLTGRPLMLFNSEGKTVWRPGQTSLWGL
                     ALSLPADTDYPDPRGERDPEADPGLLYAGQWQDAESGLCYNRFRYYEPETGMYLVSDP
                     LGLQGGEQTYRYVPNPCGYIDPLGLAICQLARWTKWGSEQSNISDVLNSLGNRALKYA
                     NGDWIKSEAAFNKYINMINKRLELTGSKFRVEIQPAIKNGERVPATTNGPFKVNGKWT
                     SGTHYTGGSKRLDAGIIDITSPTNQYGLHPVIEGFDITLNKTKPSAVDIYSDVFGGID
                     INDFRL"
     gene            7650..8057
                     /locus_tag="ACHJWA_004246"
     CDS             7650..8057
                     /locus_tag="ACHJWA_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310727.1"
                     /translation="MKEIDLLYENIYQLLIKPYLLDLSSQSGKKIESNYTCKIKDAAD
                     EIKGSMIFNDVDGKQKATCTIRVLILKTFHDGRYRFVIESVIYDLINNYSGFILTGRL
                     FWQGEGFGHELFPVTNKYNAWRWKNKKIIDISW"
     gene            8226..>9467
                     /locus_tag="ACHJWA_004247"
     CDS             8226..>9467
                     /locus_tag="ACHJWA_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zincin-like metallopeptidase toxin
                     domain-containing protein"
                     /protein_id="HFO4310728.1"
                     /translation="MTASGTGLGYGEGDESYGYDSCGYLKAQSAGGHRISEETDQYAG
                     GHRLKQAGNTQYDYDAAGRMVSRTRHRDGYRPETERFRWDSRDQLTGYCSAQGEQWEY
                     RHDASGRRTEKRCDRKKIRFTYLWDGDSIAEIREYRDDELYSVRHLVFNGFELISQQC
                     SRVRQPHPSVAPQWVTRTNHAVNDLTGRPLMLFNSEGKTVWRPGQTSLWGLALSLPAD
                     TDYPDPRGERDAEADPGLLYAGQWQDAESGLCYNRFRYYEPETGMYLVSDPLGLQGGE
                     QTYRYVPNPCGWVDPLGLAASSKISSLMDYIGDGRRVSGHTGFLDGVRLSRSQINNIA
                     KEMEKLGIKVIRKADKYLPPNARAAFDYGLRNIYLRKNATLYEVYHEVIHAKQFAKIG
                     REAYEALGRLSREEHVLNEILK"
BASE COUNT         2387 a         2394 c         2806 g         1880 t
ORIGIN      
        1 atgtgcagat catgttatca gggtggagcg gtagcatata ttttttaagc aatcattcat
       61 tttaatgatg ttaattgggt tatttagttg tgaataaaaa catattatga atattggttt
      121 aactttgcgt ttttgttact cgctgtgatg tgcgcacctg tcctgaataa cgtcaggaaa
      181 caataagttg agcttgtgat tatttttttg actgaacaga cacccgtttc tgtaaagtaa
      241 tatttaaaca aaatcctcac ataatcatag cattttttgt tccttcccta ataacgtctt
      301 aagtcgtccc gcttatctca ttatccgcag ttgtacggtg cggattatta ttcattttac
      361 cggaggttgc tatgtcaacc ggattacgtt tcacactgga agtggacggc ctgccaccgg
      421 acgcttttgc ggtggtttcc tttcatctca cccagtcact ctcttcgctc ttttccctcg
      481 atctctcctt ggtcagccag cagtttctct cccttgaatt tgcgcaggtg ctggacaaaa
      541 tggcctacct gacaatatgg cagggtgatg atgtacagcg ccgggtgaaa ggtgtggtga
      601 cctggtttga gctgggggag aacgacaaaa accagatgct gtacagcatg aaggtgcacc
      661 cgccgctgtg gcgtgccggt ctgcgccaga acttccgtat cttccagaac gaggacatca
      721 aaagcatcct cggcacgata ttgcaggaaa acggggtgac cgagtggagt ccgctgttca
      781 gtgaaccgca tccttcccgt gagttttgtg tccagtacgg tgagactgat tacgatttcc
      841 tgtgccggat ggcggcggag gaaggcatct tcttttatga ggagcatgct tacaaaagta
      901 ccgaccagag cctggtgctg tgcgacacgg tccgccatct gcccgaatct tttgaaatcc
      961 cctggaaccc gaacacccgt accgaggtga gcaccctctg catcagccag tttcgctaca
     1021 gcgcacaaat ccgcccttct tccgtggtga ccaaagacta cacctttaaa cgccccggct
     1081 gggcaggacg ttttgatcag gaaggccagc accaggatta ccagcgcacg cagtatgagg
     1141 tgtatgacta ccccggacgt ttcaagggcg cccacgggca gaactttgcc cgctggcaga
     1201 tggagggctg gcgaaacaat gcagaaaccg cgcggggaat gagtcgctcg ccggagatat
     1261 ggccgggacg gcgaattgtg ctgacggggc atccgcaggc gaacctgaac cgggaatggc
     1321 aggtggtggc aagtgaactg cacggcgagc agccgcaggc ggtgccggga aggcggggag
     1381 cagggacggc gctggagaac cattttgcgg tgatcccggc agaccggaca tggcgacccc
     1441 agccgcggct aaaaccgctg gtggacggtc cgcagagcgc cgtcgtgacg gggccggagg
     1501 gtgaggaaat cttctgtgat gaacatggcc gagtgcgggt gaaattcaac tgggaccgtt
     1561 ataacccggc agaccaggac agctcgtgct ggatccgtgt ggcacaggcg tgggcaggca
     1621 ccggttttgg caacctggcg ataccgcgtg tgggtcagga ggtgatagtg gacttcctca
     1681 acggcgatcc ggaccagccg atcattatgg ggcgcaccta ccaccaggaa aaccacaccc
     1741 ccggcagcct gccggggacg aagacgcaga tgaccatccg ttccaaaacg tatatgggca
     1801 gcgggtttaa tgagctgaag tttgatgatg cgacagggag agaacaggtc tacatccacg
     1861 cgcagaagaa catggatacc gaagtgctca acgaccgtac caccaccgta aaacacgatc
     1921 atcgcgaaac cgtaaaaaat gaccagacgg tcacgatcca ggaaggtaac cgccttctta
     1981 cggtggaaaa aggccacaag atcaccggag tactgaaagg gtctttatct gaggatgtct
     2041 ttcaggacag aggcacgatt gccggttcgg tgcatgttga cgctgtaaac aatggtggcg
     2101 aaggcaacgg tatacaggct tatacggcaa ttaaggaaat tatgctggcc gtggaggaaa
     2161 gcaaaattgc gctgacgccg gatggcattc agctacaggt cggggaatca acggtaatca
     2221 ggctgtcgaa ggatggcatc accatcgtgg gcggttctgt tttcatcaac tgattcactc
     2281 agattaacag ggagtatttt atgcagttca cttttaatga aggtcatatt caactgccat
     2341 cgcaatggca ggatcagtcg atgcaggtcc tggtatccac ggataacagt ggcatcaacc
     2401 tggtcattac ccgcgaaccc gtttcacagg gaaccctgac accagagctg taccaggaaa
     2461 cgctggcgct gtaccagggc aaacttgatg ggtacaccga acatgcctgc cgggaaatca
     2521 ctctggcaga ggccccggcc tggctgctgg attattcctg gaacggtccg gaagatgaag
     2581 ggaatcaggg gcggattagc cagatagccg tattccagcg gcgtggagat acgctgctga
     2641 ccttcacttt cagcacctcc ttatccctga agaacagtca aaagacgatg ctgctggagg
     2701 tgataaagag cttcaccccg ttgccaccgg agaatgacat ccaaaaggac cagccgcgct
     2761 gacataaacg agggataaaa catgagtgaa ggaccaggcg ggccacaggg agcgaccgca
     2821 ggcggtacgc tggcaatgcg gatgctgtcg cagcgggcga tggccgccag ccagatgaaa
     2881 cgggcagcca acgacaaagc cattgcacag atgttggcat caaaaaagtc tggccccccc
     2941 gccgccaggc tgggcgatga aattcagcac aagagttttt tgggggcgct ggcaggggcc
     3001 gtgctggggg cgatagtgac catagcagaa ggttgcctga ttatggccgc ctgtgccacc
     3061 ggcccttatg cgctggttct ggtgcctgcg ctgatgtatg ccagctataa ggcgagtgat
     3121 tatgtggagg agaaacagaa ccagcttgaa tcatggataa acagcttttg tgacacggac
     3181 ggcgccatca ataccggttc taaaaatgta aaaattaacg gaaagccagc agcccgtgca
     3241 gccgtcaccc ttccccctcc tcccccacct ggagcaatac ctgaagtccc acagggggaa
     3301 ccctcatggg gtgatattgc cactgacctg cttgaatcgg cagcggaaaa agcagtacca
     3361 ctggcgaagg cctgggggaa cgctgttatc accctgacgg acagcaatgc cggttttatg
     3421 gatcgcgtca gcgccggcgc atcgcttctg tttcccgccg gtccggtatt aatggagttt
     3481 gccaccatgg tgggcgggcg tggcgaaatc aaaaaagatg tggatttccc ggaagccggt
     3541 gaggacacgg cgctctgcga caaggagaac aaaccaccga ggatagccca gggcagcagc
     3601 aacgtcttta tcaacaatca gcctgccgcg cgcaagggcg acaaactgga gtgcagcgcg
     3661 gcaatcgtgg aaggttcgcc ggacgtcttt attgggggtg agcaggtcac ctatctggat
     3721 atccagccgg agttcccgcc atggcagaga atgatcctgg gaggaataac gatagccagc
     3781 tatcttctgc cgccagcagg actgctggga aaactgggga atctggcgaa actgggcaaa
     3841 ctgggaaacc tgctggggaa aagcggtaag ctgctgggcg caaagctcgg cgcgttgctg
     3901 gggaaaacag gtaattcgtt aaaaagtatt gccaataaag tcatcagatg ggtaacagat
     3961 cctgtcgatc cggtaaccgg cgcatactgc gacgaacgta ccgactttac gctgggccag
     4021 acactccccc tctccttcac ccgtttccac agttctgtac tgccgctgca tggcctgacg
     4081 ggcgtgggct ggagcgactc ctggagcgaa tacgcctggg tgcgtgaaca gggaaaccgg
     4141 gtggatgtca tcagcctggg agccacgctg aacttcgcct tcgacggtga aagtgatacg
     4201 gcggttaacc cgtatcacgc ccagtacatt ctgcgccgcc gtgatgatta tctggagctg
     4261 ttcgacaggg atgcactgag cagccgcttc ttttatgacg cctttccggg aatgcgtctg
     4321 cgccacccgg tgactgacga taccagcgat gaccgcctgg cacacagccc cgcagaccgg
     4381 atgtacatgc tgggcgggat gagcgacacc gccagcaacc gcatcacgtt tgagcgcgac
     4441 acccagtacc ggatcacggg tgtcagtcac accgacggga tccggcttaa actgacgtac
     4501 cacgccagcg gctacctgaa agccattcac cgcacggata acggcataca gacgctggcg
     4561 acctacgaac aggatgcgcg gctggactac cacctgtttt atgagtacga cgctgcggac
     4621 cggatcatcc gctggtccga taacgaccag acgtggagcc gtttcaccta cgatgcacag
     4681 ggccggtgcg tgaccgtcac cggggcggag ggctattaca acgccacgct ggactatggt
     4741 gacggctgca ccaccgtgac ggacggcaag ggcattcacc gttattacta tgatcctgac
     4801 ggcaatattc tgcgggaaga agcgccggac ggcagcacca ccacgtatga atgggatgaa
     4861 ttccatcacc tgctggcccg ccactcccct gccgggcggg tggagaagtt tgaatacaac
     4921 gccgcacacg gtcagttaag ccgttacacg gcggcagacg gcgcggattg gcagtactgc
     4981 tatgatgagc gcggcctgct cagcaacatc accgcccctg ccgggcagac gtggacgcag
     5041 cagtgtgatg aacgcggcct gccggtgagt ctggtatcgc cacagggcga agagacccgg
     5101 ctggcgtaca cccctcaggg gctgctgtcg gggatattcc gccaggatga acggcgtctg
     5161 ggcatagagt acgaccacca caactggccg gaaacactca ccgacgtgat gggccgcgaa
     5221 caccacaccg aatacagcgg tcacgacctg ccggtgaaga tgcgcggccc cggcggtcag
     5281 tcagtgcggt tgcagtggca gcagcaccat aaactgagtg gccttgagcg ggcaggaacc
     5341 ggcgcggaag gattccgcta cgaccgccac ggcaacctgc tggcgtacac ggacggtaac
     5401 ggcgttgtct ggacaatgga gtacggcccg tttgatttgc cggtggcgcg aacggacggt
     5461 gaaggccacc gctggcagta ccgctacgat aaagacacgc tgcaactgac agaagtcatt
     5521 aacccgcagg gcgagtctta tctttatatt ctggacaact gtggccgggt gacggaagaa
     5581 cgtgactggg gcggcgtggt ctgtcgttac cgttatgacg ctgatggcct gtgtaccgcc
     5641 agggtcaacg gcctggagga aaccatcctc tacagccggg atgccgcagg ccgcctggca
     5701 gaagtcatca ctccggaagg caaaacgcag tatgcgtatg acaaatccgg caggctgacg
     5761 ggtatcttca gcccggacgg cacatcacag cgcaccggct atgatgaacg cgggcgggtg
     5821 aatgtcacca ctcagggccg acgggccatt gaataccact accccgacga acacaccgtc
     5881 atccgctgta tcctgccacc ggaagatgaa cgcgacagac accccgacgg atccctgctg
     5941 aaaaccacat accgctacaa cgccgccgga gaactgacgg aggttatcct gccgggggat
     6001 gagacgctga cgttcagccg tgatgaggcg ggacgtgaag tgctccggca cagtaaccgg
     6061 ggttttgcct gtgaacaggg ctggaatgca gccggtcagc ctgtcagcca gcgcgccgga
     6121 cttttcccgg cggaagccac atggggcgga ctgctccctt cactgctacg ggaataccgt
     6181 tacgacagcg cgggtaacgt atcaggcgtc accagccggg aagattacgg acgggaaaca
     6241 caccgggagt accggcttga ccggaacggc caggtcacgg cggtgacagc ctcaggcacc
     6301 gggctgggct atggcgaagg cgacgagact tatggctatg acagctgcgg ctacctgaag
     6361 gcgcagtctg cgggcagaca ccggataagc ggagagactg accagtatgc cgcaggccac
     6421 cggctgaaac aggccggaaa cacacagtat gactatgacg ccgcaggccg gatggtcagc
     6481 cgcacaaaac accgtgacgg ctaccgccca gaaacagagc ggttccggtg ggacagccgg
     6541 gaccagctga ccgggtatcg cagcgcacag ggggagcagt gggaataccg ccacgacgcc
     6601 agcggcagac ggacggaaaa acgctgcgac cggaagaaaa tccgttttac gtacctgtgg
     6661 gacggcgaca gtattgcgga aatccgggaa taccgcgatg ataaactgta cagcgtaagg
     6721 cacctggtgt ttaacggctt tgagctgata agccagcagt tcagccgggt acgacagccg
     6781 catccgtccg tggccccgca gtgggtgacg cgaacgaatc atgcggtgag cgacctgacg
     6841 ggccgcccgc tgatgctctt taacagtgaa ggtaaaaccg tctggcggcc ggggcagacc
     6901 agcctgtggg ggctggcact cagcctgccc gcagacacag actacccgga cccgcgcggg
     6961 gagcgggacc cggaagcgga ccccggcctg ctgtatgcgg gacagtggca ggatgcagaa
     7021 tcggggctgt gctataaccg gttccggtac tacgagccgg aaaccgggat gtacctggtg
     7081 agtgatccgc tggggttgca gggaggggag cagacttatc ggtatgtgcc gaatccttgt
     7141 gggtatatcg atcctttggg gctggctata tgtcagttag cccgctggac gaaatggggg
     7201 agtgagcaaa gcaacatatc tgatgttttg aactcattag ggaatagagc acttaaatat
     7261 gctaatggtg attggataaa atcagaggct gcattcaata aatacataaa catgataaat
     7321 aaaagactag aattaacagg tagtaaattt agagttgaga ttcaaccagc cataaaaaat
     7381 ggagagcgag ttcctgcgac aacgaatgga ccatttaaag taaatggtaa gtggacatcc
     7441 ggcactcatt atacaggtgg ttccaaacgt ctagatgccg gtattattga tatcacatct
     7501 cctacaaacc aatatggatt acatccagtt attgaaggat ttgatataac acttaataaa
     7561 acaaaaccat cagcagtgga tatatattca gatgtgtttg gtgggattga tattaacgac
     7621 tttcggttat aaaacaataa ggcggcaata tgaaagagat tgatttatta tatgaaaata
     7681 tttatcagct tttaattaaa ccttacctac tcgatctttc tagccagtct ggaaaaaaaa
     7741 tagaatcaaa ttacacatgc aaaataaaag atgctgctga tgaaataaag ggtagtatga
     7801 tttttaatga tgtagatggt aaacaaaaag caacttgcac tatcagggtg ttaatactaa
     7861 aaacatttca cgatggtcgt tatagatttg taattgaatc tgtgatttat gatttaatta
     7921 ataattacag tggatttatt ttaacgggga gattgttttg gcaaggggaa ggttttggcc
     7981 atgaattatt ccccgtgaca aacaaatata atgcttggag atggaaaaat aaaaaaataa
     8041 tagatatttc ttggtaagga aaatattccc cgaaagaggc cacatgggga atactgctcc
     8101 cctcactgat acgggagtac cgttacgaca gcgcgggtaa cgtatcaggc gtcaccagcc
     8161 gggaagatta cggacgggaa acacagcggg agtaccggct tgaccggaac ggccaggtca
     8221 cggcggtgac agcctcaggc accgggctgg gctatggcga aggcgatgag tcctatggct
     8281 atgacagctg tggctacctg aaggcgcagt ctgcgggcgg gcaccggata agtgaagaga
     8341 ctgaccagta tgccggaggc caccggctga aacaggccgg aaacacgcag tatgactatg
     8401 acgccgcagg ccggatggtc agccgcacca gacaccgtga cggctatcgc ccggaaacag
     8461 agcggttccg gtgggacagc cgggaccagc tgaccgggta ttgcagcgca cagggtgagc
     8521 agtgggaata ccgccacgac gccagcggca gacgaacaga gaaacgctgc gaccggaaga
     8581 aaatccgctt cacgtacctg tgggacggcg acagtattgc ggaaatccgg gaataccgcg
     8641 atgatgaact gtacagcgta cggcacctgg tgtttaacgg ctttgagctg ataagccagc
     8701 agtgcagccg ggtacggcag ccgcacccgt ccgtggcccc gcagtgggtg acacgaacga
     8761 atcatgcggt gaacgacctg acgggccgtc cgctgatgct ctttaacagt gaaggtaaaa
     8821 ccgtctggcg gccgggacag accagcctgt gggggctggc actcagcctg cccgcagaca
     8881 cagactaccc ggacccgcgc ggggagcggg acgcggaagc cgaccccggc ctgctgtatg
     8941 caggacagtg gcaggatgca gaatcggggt tgtgctataa ccggttccga tattacgagc
     9001 cggaaaccgg gatgtacctg gtgagtgatc cactggggtt gcagggaggg gagcagactt
     9061 accggtatgt gccgaatcct tgtgggtggg ttgatccgct gggattggct gcaagttcta
     9121 aaatcagcag tttgatggac tatattggcg atggtcgtcg tgttagtggg catacgggtt
     9181 tcctggatgg ggttcgttta tcacgtagtc aaataaacaa tattgctaaa gaaatggaga
     9241 agctaggaat taaagtaata aggaaagcag ataaatattt gccaccaaat gctagggcag
     9301 cttttgatta tggtcttcgc aatatttatc ttaggaaaaa tgctacctta tatgaggtgt
     9361 atcatgaagt gattcatgct aagcaatttg cgaaaattgg acgagaagca tacgaagcac
     9421 taggacgttt atctagggag gaacacgttc taaatgaaat attaaaa
//
LOCUS       DAZFAB010000145         9381 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZFAB010000145 DAZFAB010000000
VERSION     DAZFAB010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9381
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.145"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(11..511)
                     /locus_tag="ACHJWA_004248"
                     /pseudo
     CDS             complement(11..511)
                     /locus_tag="ACHJWA_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535762.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
     gene            complement(1170..1352)
                     /locus_tag="ACHJWA_004249"
     CDS             complement(1170..1352)
                     /locus_tag="ACHJWA_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310729.1"
                     /translation="MLLAENPRQGMTTEARVVIRILLISDHVNRQDNTQIQTIMRIIY
                     ICLLQKIKIIDKTKYK"
     gene            1291..3381
                     /gene="iha"
                     /locus_tag="ACHJWA_004250"
     CDS             1291..3381
                     /gene="iha"
                     /locus_tag="ACHJWA_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HFO4310730.1"
                     /translation="MRITTLASVVIPCLGFSASSIAAAEDVMIVSASGYEKKLTNAAA
                     SVSVISQEELQSSQYHDLAEALRSVEGVDVESGTGKTGGLEISIRGMPASYTLILIDG
                     VRQGGSSDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITRKN
                     ADKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQVRGSTQQRQGSSVTSL
                     SDTAATRIPYPTESQNYNLGARLDWKASEQDVLWFDMDTTRQRYDNRDGQLGSLTGGY
                     DRTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKRDKWGLAGQPRE
                     LKESNLILNSLLLTPLGESHLVTVGGEFQSSSMKDGVVLASTGETFRQKSWSVFAEDE
                     WHLTDALALTAGSRYEHHEQFGGHFSPRAYLVWDVADAWTLKGGVTTGYKAPRMGQLH
                     KGISGVSGQGKTNLLGNPDLKPEESVSYEAGVYYDNPAGLNANVTGFMTDFSNKIVSY
                     SINDNTNSYVNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSY
                     TPEHMVNAKLNWQITEEVASWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAW
                     TVVDAGLSWKMTDALTLNAAVNNLLNKDYSDVSLYSAGKSTLYAGDYFQTGSSTTGYV
                     IPERNYWMSLNYQF"
     gene            complement(3599..3749)
                     /locus_tag="ACHJWA_004251"
                     /pseudo
     CDS             complement(3599..3749)
                     /locus_tag="ACHJWA_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(3961..4281)
                     /locus_tag="ACHJWA_004252"
                     /pseudo
     CDS             complement(3961..4281)
                     /locus_tag="ACHJWA_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053346.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
     gene            complement(4704..5279)
                     /locus_tag="ACHJWA_004253"
     CDS             complement(4704..5279)
                     /locus_tag="ACHJWA_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO4310731.1"
                     /translation="MAVSLVKGGNVSLTKEAPTMNVAMVGLGWDARVTDGQGFDLDAS
                     VFAVGEDGKVLSDAHFIFFNNKTSPDGAVEHQGDNRTGEGDGDDEQVKIDLTKVSADI
                     KKLVFAVTIYDAEARKQNFGMVSNSFMRVYNNDNGTEIARFDLSEDASTETAMVFGEL
                     YRHGTEWKFKAVGQGFAGGLSALASQHGVNV"
     gene            complement(5348..5926)
                     /gene="terD"
                     /locus_tag="ACHJWA_004254"
     CDS             complement(5348..5926)
                     /gene="terD"
                     /locus_tag="ACHJWA_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerD"
                     /protein_id="HFO4310732.1"
                     /translation="MSVSLSKGGNVSLSKAAPSMKNVLVGLGWDARSTDGQDFDLDAS
                     AFLLASNGKVRGDSDFIFYNNLTSSDGSVTHTGDNRTGEGDGDDESLKIKLDAVPSEV
                     DKIIFVVTIHDAQARRQSFGQVSGAFIRLVNDDNQTEVARYDLTEDASTETAMLFGEL
                     YRHNGEWKFRAVGQGYAGGLASVCAQYGINAS"
     gene            complement(5975..7015)
                     /gene="terC"
                     /locus_tag="ACHJWA_004255"
     CDS             complement(5975..7015)
                     /gene="terC"
                     /locus_tag="ACHJWA_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerC"
                     /protein_id="HFO4310733.1"
                     /translation="MVSTHIGFPTETVIVFIALSVGAIFIDLFMHRDDKPISLKSAAL
                     WSVFWVVVAMAFAGFLYIHHGAEVASLFVTGYALEKVLSVDNLFVMMAIFSWFAVPDR
                     YRHRVLYWGIIGAIVFRGIFVAIGTSLLSLGPYVEVVFAIIVAWTAVMMLKSGDDDDE
                     IEDYSQHLAYRMVKRFFPIWPKLRGHAFLLNQKEVDAELAKPENSDVTIGRGKKAALY
                     ATPLFLCVAVVELSDVMFAFDSVPAIIAVSREPLIVYSAMMFAILGLRTLYFVLEALK
                     QYLVHLEKAVIVLLFFIAAKLGLNATDHIWHHGYSIAATTSLYVVLGVLALGILASVM
                     FPGKPESEEKGS"
     gene            complement(7038..7493)
                     /locus_tag="ACHJWA_004256"
     CDS             complement(7038..7493)
                     /locus_tag="ACHJWA_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFO4310734.1"
                     /translation="MSFFDKVKGAINSGRDELTRQVGRFKNKKFMQGTVAVCARIAVS
                     SDGVSSEEKQKMMGFLRSSEELKVFDTNEVIEFFNKLVSSFDFDVEIGKGETMKYILA
                     LKDQPEAAQLALRVGIAVAKSDGNFDQDEKLASREIAIALGFDPAEFGL"
     gene            complement(7516..8673)
                     /locus_tag="ACHJWA_004257"
     CDS             complement(7516..8673)
                     /locus_tag="ACHJWA_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO4310735.1"
                     /translation="MNLTPGGNAPVPAQELRVRITSGGQVDASAFRLYADGKVQGDAD
                     MVFYGQPRNDDGTVSLVSEGQYSTFTVALNRLKPDVQKIAFTVTCDGGQTVSGLRNLS
                     IDVEQGATGLVSGSVELSGRQEAALILGEFYRRNNDWKFRFVAQGFNGGLKPLAEHFG
                     VNIADEPAPAAPTPPVVTPPPVETKISLSKVSLTKEKPAISLTKRDNFGEIRINLNWH
                     RGSSKSGFAGMFGSKGIDLDLGAFVELQDGYKSVIQALGNAFGDYRDEPYVQLKGDDR
                     TGDVSDGEWLHINGREWKHIREVLIYAFIYEGVPSWDKTDGVVTIHVPDQPPIETRLT
                     EGENRRTLCAIARLVNENGAIKVERINQYFKGQDEMDRAFGWGFRWSAGSK"
     gene            complement(8673..9254)
                     /locus_tag="ACHJWA_004258"
     CDS             complement(8673..9254)
                     /locus_tag="ACHJWA_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO4310736.1"
                     /translation="MVSLVKNQTVSLSKESSALSQLHFGLGWDPVKKKGLLGGLFGGN
                     DSIDLDAGCVLMDSTGKTIDTIWFRKLKSTCGAVVHSGDNLTGEGDGDDEVIKVNLSR
                     LPANVEYLAFTVNSFRGQSFNDVENAFCRVVDQTGKELARYKLTEQGSHTGIVISSLR
                     RNNGNWDFTALGHACRGRTIDDMHSDIVSAVIR"
BASE COUNT         2453 a         2316 c         2277 g         2335 t
ORIGIN      
        1 aaagattcta acaaagcatt tcaacaatat atacttgtta aaaatccata tcgaaatgcc
       61 gttgcagcat taatatatgc ctcattcata aaatgtaaaa gagcaagctc gtaccagtag
      121 gggggaaatt cattgacatg tcctgtcaat acgtacagag ccctgccatg cttgccagca
      181 cgtaacaatt ccgcccccag taaccagcgt gttccctgat tattctcagg gttatatgtt
      241 agtattttat caatgagtat aacggcatcc tgatgacgct gtaaatgaac attcgccaga
      301 attgcagcat aaagagcgca aatgcatatg ccagatgagc gtgaatatca atgatgtcag
      361 gagcatgtca ggcaagacgc ctgagtttgt tgacgtaatt tgtttcgttt atttcatcac
      421 tatcgtatgc gtcaagagtt ttttcaaact catcccgaaa tgggccaagc ctggtttcag
      481 gaaataagaa atacccttgg ttattgttca cttaaaagta taatttgtaa ttcaaatcct
      541 gagcctgtaa agcggggagt aacatattct attctgaaga gaataaaagt cgtgatgcga
      601 tgtatcaagc ccggattgta atcccagatt aacatagatc acaaaacaac ttattcttca
      661 ctaacgtcaa gataaatatc gttgtatgcc ttatcacgac tacgaatacc agcaagatac
      721 aactgacata cggaaagata ccacgttttt tactcccgaa aataacgcta aaaagctact
      781 tccccatcgt ttgtccttag tattgccagc gccaacaatg tgggctgaca tgataaagct
      841 gtctaggaaa ttgttcgcct cctcagcgga caatccaaat ggtgattgtc tctgttaaac
      901 gtttattttg aaggtcgact gaataaggtg atgacgctgt agaatttttc acgtgccaca
      961 gaattttgaa cgctttctct tacaatattt caatgtttct atcagtattc gccggaagaa
     1021 gtcatcgacc aaatcatccc agtcgtctcg catcactgac cattcatggt tgacatgtgg
     1081 tggaaatccg cttctacagt aaccattttt tattcgcaaa accgaacacg ccatacggat
     1141 agctgttaac tggcatgccg aggcagtcgt tatttatatt tggttttgtc aataatcttt
     1201 attttttgta aaaggcaaat ataaattatt ctcattattg tttgtatttg tgtattgtct
     1261 tgccggttaa catgatcgga gattagtaat atgcgaataa ccactctggc ttccgtagtc
     1321 attccctgtc tcggattttc agccagcagc atagctgctg cagaggatgt gatgattgtc
     1381 tcggcatccg gctatgagaa aaagctgact aacgcagccg ccagtgtttc tgtgattagc
     1441 caggaggaat tgcagtccag ccagtaccac gatctggcgg aggctctgag atcagtagag
     1501 ggtgtggatg ttgaaagtgg tacgggtaaa accggagggc tggaaatcag catccgagga
     1561 atgccagcca gttacacgct gatactgatt gatggtgttc gtcagggcgg aagcagtgac
     1621 gtgactccca acggtttttc tgccatgaat accgggttca tgccccctct ggccgccatt
     1681 gagcgtattg aggttatcag ggggccgatg tccacactgt atggctctga tgcgatgggc
     1741 ggtgtggtga atatcattac cagaaagaat gcagacaaat ggctctcttc cgtcaatgca
     1801 gggctgaatc tgcaggaaag caacaaatgg ggtaacagca gccagtttaa tttctggagc
     1861 agtggtcccc ttgtggatga ttctgtcagc ctgcaggtac gcggtagcac acaacagcgt
     1921 cagggttcat cggtcacatc actgagcgat acagcagcca cgcgtattcc ttatcccacg
     1981 gagtcacaga attataatct tggtgcacgt cttgactgga aggcgtcgga gcaggatgtg
     2041 ctctggtttg atatggatac cacccggcag cgttatgata accgggatgg gcaactgggg
     2101 agtctgacgg ggggatatga ccggaccctg cgctatgagc gaaacaaaat ttcagctggc
     2161 tatgatcata ctttcacctt cggaacatgg aaatcgtatc tgaactggaa cgagacagaa
     2221 aataaaggtc gtgagcttgt acgcagtgta ctgaagcgcg acaaatgggg gcttgccggt
     2281 cagccgcggg agcttaagga atcgaacctt atcctgaatt cattactgct tacccctctg
     2341 ggagaatctc atctggttac ggtggggggc gagtttcaga gctcgtccat gaaagacgga
     2401 gttgtccttg ccagcacagg tgaaactttc cggcagaaaa gctggtcggt atttgctgag
     2461 gatgagtggc atctcacgga tgcacttgcg ctgactgcgg gcagccgcta tgaacatcat
     2521 gagcaattcg ggggacactt cagtccgcgt gcatatctgg tctgggatgt ggcagatgcc
     2581 tggacgctga aaggcggtgt gaccacggga tataaggcac ccagaatggg gcagctacat
     2641 aaagggatta gtggtgtgtc cgggcaggga aaaacaaatc tacttggtaa ccccgacctg
     2701 aagccggaag agagcgtcag ttatgaggct ggggtgtatt acgataaccc cgccggtctg
     2761 aatgccaatg tcacaggttt tatgactgac ttctccaaca agattgtctc ttattccata
     2821 aatgataaca ccaatagcta tgtaaacagc ggaaaggccc ggttgcacgg tgtggaattt
     2881 gccggcacat tgccgctgtg gtcagaggat gtcacgctgt cactgaatta cacctggacc
     2941 cgaagtgaac aacgtgatgg tgataacaaa ggtgcgccgc tgagttatac ccctgaacac
     3001 atggtgaatg cgaaactgaa ctggcagatc accgaagagg tggcatcatg gctgggtgcc
     3061 cgttatcgcg ggaaaacacc acgtttcacc cagaattatt cgtcactgag cgctgtacag
     3121 aagaaagtgt atgatgagaa aggagaatac ctgaaagcct ggacggtggt ggatgcaggt
     3181 ctgtcgtgga agatgacgga tgccctgacg ctgaatgctg cggtgaataa cctgctcaac
     3241 aaggattaca gtgacgtgag cctgtacagt gccggtaaga gtacgctgta tgccggtgat
     3301 tacttccaga cgggatcatc aacaacagga tatgtgatac ctgagcgaaa ttactggatg
     3361 tcgctgaact atcagttctg ataataacaa aacgctatca ctgacggtag aatacgttgc
     3421 cactgcaact cctggcggaa cagtggcaac gtattaggtt aagtgcattt ccgatccgct
     3481 aatgagattt cgttaccaac aactaatatc gtcacaggaa atgcacggat tatttttaac
     3541 ttatcattta catacttgtc cagagtgtaa gcgcaccgtg aaggacgtgg ggtaaaaatt
     3601 agtttacaga gagagtgacg ttccagggca acaactcttt catgcggttg gcaggccagg
     3661 tgttgattac actgatcacg tggcgtagcc acgtttccgg ctcgattccg ttaagttttg
     3721 cagctaccga tcaggctgta catcactgcc gcactatcgc tcgtcatctc aaagtccggt
     3781 ctcgtcagca ggaaggtatc attctctccc gccatttttc caggggcccg gtcagataag
     3841 tccctttgtc tatcgctgac tcctgactca taacccggtt agcagaatgc agggtcacca
     3901 cctcgccacg accaaatcca aataagtcaa ttgcaccttc tcaatcgcca ttttgtcagt
     3961 aagcgtacag cctgaactga tggtatcttc accatcaatg acaacggtga tcgcaatttt
     4021 actgacgttc gccggaacac gatccagtgc tatctcaatg ctggcctgct gcgaaccggt
     4081 aacgagcctg actgccccct caggagaaga caaattatta taaaagataa agtcagaatc
     4141 gccactgacc tttccctgag cattaagcat gaacaggcag gtatcgggtt cgcctttaaa
     4201 gccggatttg gcagggtact gaagattcag cctgatcgta gattgctgaa ggggtatgtt
     4261 ttgtccggat tgtaaattca tattaactct cctgattttt gattattatt aatgcgcagc
     4321 gtttatatat gttccctagg cttagttctg gacgctggat attcggtgag gcgtaaatat
     4381 ggtatgacac catttttcat aacgctgaag tttctatacc tgttgaattt gaattttcat
     4441 tgaccgggta tcttattttc cagggcccgt tccttcataa gtcgcaaaag taacatatat
     4501 ccgaagggca tgctgttgat atcagacacg gaatactggc tttaaccagc aaccacagat
     4561 aaacccgggg cctgcataaa aggttcatgc cagaattaac atagctctgc tttctgcccc
     4621 ccccctttcc catattcacc cgttgatagc ggatcgatac ccaaaaaaaa ccccggcatt
     4681 gccggggtca gactcagcgt cttttagaca ttgacgccgt gctgggaagc aagcgctgac
     4741 agaccaccgg caaaaccctg gccaacagct ttaaacttcc actcagtacc atggcgatac
     4801 agttcaccga agaccattgc ggtttcggtt gaggcgtctt cagacagatc gaaacgggca
     4861 atttccgtcc cgttgtcgtt gttgtaaacg cgcatgaagc tgttgctcac catgccgaag
     4921 ttttgtttac gcgcttctgc atcatagatg gtaacggcaa ataccagttt tttgatgtct
     4981 gctgagactt tggtcagatc gattttgacc tgctcatcgt cgccgtcgcc ttcaccggta
     5041 cggttgtcgc cctggtgctc tactgcgcca tcagggctgg ttttattatt gaagaaaatg
     5101 aaatgggcat ctgacagtac tttaccgtct tcacctactg cgaatacgga agcgtccaga
     5161 tcaaaaccct gaccatcggt tacacgggca tcccagccca ggccaaccat agcgacattc
     5221 atggttggtg cttctttggt cagagatacg ttgccgcctt ttacgagaga aactgccatt
     5281 tttagctcct gcaaacagtt gaatgaggct gaattaacag ccccagatat gaaaagttac
     5341 ttttcgatca ggacgcgtta atgccgtact gagcacatac agatgccaga ccaccagcat
     5401 aaccctggcc tactgcgcgg aatttccact caccattgtg gcgatacagc tcgccgaaca
     5461 gcatggcagt ctcagtggac gcatcttcgg tcagatcgta gcgagcgact tcagtctggt
     5521 tatcgtcatt aaccagacga ataaacgcac cggatacctg accaaagctc tggcgacgag
     5581 cctgagcatc gtggatggtc acaacgaaga tgatcttgtc aacttcagac gggacggcgt
     5641 ccagtttaat tttcagcgat tcatcatcac catcgccctc accggtgcgg ttatcgccgg
     5701 tgtgcgttac ggaaccgtcg gatgacgtca ggttgttata gaagatgaaa tctgaatcgc
     5761 cgcgcacttt gccgtttgag gccagcagga atgctgaagc atccaggtca aagtcctgac
     5821 cgtctgttga acgcgcatcc cagccaaggc ccaccaggac atttttcatt gacggagctg
     5881 ctttactcag ggagacgttc ccgcctttgg aaagagaaac actcataaaa taacctcttc
     5941 gattagtaat tgttcaggtt aacacttaag gggattaact ccccttttcc tcagattcag
     6001 gtttgcccgg gaacatgacg cttgcgagaa tgcccagcgc cagtacaccc agtacaacat
     6061 acaggctggt tgttgccgcg atgctgtaac catgatgcca gatgtgatcg gtcgcattca
     6121 ggccgagttt tgccgcgatg aagaacagca gcacgataac ggccttctcc agatgaacca
     6181 ggtactgttt cagtgcctca aggacaaaat acagagtacg cagacccagg atagcaaaca
     6241 tcatggcact atagacgata agcggttcac gactgacggc aatgattgcc ggtaccgagt
     6301 caaacgcgaa cattacgtcc gagagttcaa ccacagccac acacaggaac agcggggtcg
     6361 catacagcgc cgctttttta ccacggccaa tggtgacatc gctgttttct ggtttcgcca
     6421 gttcagcatc cacttccttc tggttaagta ggaaggcatg ccctctgagc ttcggccaga
     6481 tagggaagaa gcgtttaacc atacggtaag ccagatgctg ggagtaatcc tcaatttcat
     6541 catcgtcatc accgctttta agcatcatga ccgccgtcca ggcaacgata atagcgaaga
     6601 caacttcaac gtacggcccc agactcagca ggctcgtacc gatggcgaca aagatgcccc
     6661 tgaagacgat ggcaccaatg atcccccagt agagaacacg gtggcgataa cgatccggaa
     6721 cggcgaacca ggagaagatt gccatcatga cgaacaggtt atcgaccgac agcactttct
     6781 ccagcgcata acccgtgaca aacagactgg caacctcagc accgtggtgg atatagagga
     6841 aaccggcaaa tgccatcgca accacaaccc agaatacgga ccagagcgca gcactcttca
     6901 gcgagattgg cttgtcatca cggtgcataa acaggtcaat aaagatggca ccgactgaaa
     6961 gcgcaataaa aacaatgacc gtttcagtcg ggaagccgat gtgtgtggaa accatatttt
     7021 acccttaggg atgtggatca gaggccaaat tcagccgggt cgaagcccaa cgcgatagcg
     7081 atctcgcggg aggccagttt ctcgtcctga tcgaagttac cgtcactttt cgcaacggca
     7141 ataccaacac gtaaggccag ctgagcggcc tcaggctgat ctttcagcgc caggatgtat
     7201 ttcatggttt cgcccttgcc aatttcaaca tcgaaatcga agcttgaaac cagtttattg
     7261 aagaactcaa tcacctcatt ggtatcgaag accttcagct cttctgaaga gcgcagaaag
     7321 cccatcattt tctgcttttc ttccgaactt acgccgtcac tcgatacggc aatacgggca
     7381 catacagcaa cggtgccctg catgaatttt ttgtttttga aacggccaac ctggcgggtc
     7441 agttcgtcac ggcctgagtt aatggcacct ttaactttgt cgaaaaagct catacctgtt
     7501 tcctttgttg ctgtgttatt tagaaccggc gctccagcga aatccccagc caaatgcccg
     7561 gtccatttcg tcctggcctt tgaagtactg gttaattcgc tcgactttaa ttgcgccgtt
     7621 ttcgtttacg agtctggcaa tggcgcacaa tgtgcgacgg ttctcacctt cggtcaggcg
     7681 ggtctcaatg ggcggttgat ccggcacatg aatggtgact acaccatcag tcttgtccca
     7741 gctgggaaca ccttcataaa taaaggcgta aatcagcact tcgcggatat gcttccattc
     7801 acgtccattg atatgcagcc actcgccgtc tgatacatca cccgtccggt catcgccctt
     7861 gagctgaaca tacggttcat cgcgataatc accgaatgca ttaccgagcg cctgaatgac
     7921 cgatttatac ccgtcctgaa gctcaacaaa ggcccccaga tccaggtcga ttcctttgga
     7981 gccgaacata cctgcaaagc ctgatttgct actgccccgg tgccagttca ggttgatccg
     8041 gatttcaccg aagttatccc gtttggtcag gctgattgca ggcttctctt ttgtcagcga
     8101 taccttgctc aggctgattt ttgtctcaac tggcggaggg gttacaaccg gaggagttgg
     8161 ggctgcggga gccggttcgt cggcaatatt tacgccgaaa tgctctgcca gcggtttaag
     8221 tccaccgttg aacccctgcg caacgaagcg gaatttccag tcattattac gacggtaaaa
     8281 ttcccccaga attaaggctg cttcctgacg gccgctcagt tcgacactgc cgctaaccag
     8341 gccggtagcc ccttgctcca cgtcgatgga aagattacga agaccggaca ccgtctgccc
     8401 accgtcacag gtcacggtga acgcaatttt ctggacatca ggtttcagcc ggttgagcgc
     8461 gacagtaaag gttgagtact ggccttcact gaccaggctg acggtaccat catcgttacg
     8521 tggctgacca taaaacacca tgtccgcatc accctgcacc ttcccgtcgg catacaggcg
     8581 aaacgccgaa gcatccacct ggccgcccga ggtgatccgg acccgcaact cctgggctgg
     8641 aacgggggca ttgccccccg gcgtcaggtt cattagcgaa taaccgctga aacgatatcg
     8701 gagtgcatat cgtcaattgt gcggccgcga caggcatggc caagggcggt aaagtcccag
     8761 ttgccgttat tacggcgcag cgaagaaata acgatcccgg tatgtgagcc ctgctcagtc
     8821 agcttgtagc gagccagctc tttgccggtt tgatcgacaa cgcggcagaa agcgttttcg
     8881 acgtcattga acgactgacc acggaagctg tttaccgtaa acgccaggta ttcaacgttt
     8941 gcaggcaggc gggagaggtt gaccttaatc acttcatcgt caccgtcacc ctcaccggtc
     9001 aggttatcgc cactgtgcac aacagcgccg caggtggatt tcagtttgcg gaaccagatg
     9061 gtgtcaattg ttttacctgt gctgtccatt aatacacagc cagcatcgag atcgattgag
     9121 tcgttgcctc caaacaatcc tccaagaaga ccttttttct taaccggatc ccaaccgaga
     9181 ccgaaatgaa gctggcttaa tgcagatgat tctttgctga gtgataccgt ctggttctta
     9241 actaatgaaa ccatgtttta ttccttaaaa tggtaatgac cgagacaatc aaaggtatat
     9301 ttagtaactg acgaagaaaa tcatatcatg atgaagttct ctgacaaggg aaatttttgt
     9361 ggttttctgt aaaaacacaa a
//
LOCUS       DAZFAB010000146         9201 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZFAB010000146 DAZFAB010000000
VERSION     DAZFAB010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9201
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.146"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(1048..1578)
                     /gene="yodB"
                     /locus_tag="ACHJWA_004259"
     CDS             complement(1048..1578)
                     /gene="yodB"
                     /locus_tag="ACHJWA_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFO4310737.1"
                     /translation="MNRFSKTQIYLHWITLLFVAITYAAMELRGWFPKGSSTYLLMRE
                     THYNAGIFVWVLMFSRLIIKHRYSDPSIVPPPPAWQMKAASLMHIMLYITFLALPLLG
                     IALMAYSGKSWSFLGFNVSPFVTPNSEIKALIKNIHETWANIGYFLIVAHAGAALFHH
                     YIQKDNTLLRMMPRRK"
     gene            complement(1921..2571)
                     /gene="zinT"
                     /locus_tag="ACHJWA_004260"
     CDS             complement(1921..2571)
                     /gene="zinT"
                     /locus_tag="ACHJWA_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFO4310738.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLAPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(2828..3463)
                     /gene="msrQ"
                     /locus_tag="ACHJWA_004261"
     CDS             complement(2828..3463)
                     /gene="msrQ"
                     /locus_tag="ACHJWA_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFO4310739.1"
                     /translation="MRLTAKQVTWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWIILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKSRSLFNRLRKQVHNKLSV"
     gene            complement(3464..4468)
                     /gene="msrP"
                     /locus_tag="ACHJWA_004262"
     CDS             complement(3464..4468)
                     /gene="msrP"
                     /locus_tag="ACHJWA_004262"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFO4310740.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKALEFSKPAAWQNNLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(4576..4989)
                     /gene="hiuH"
                     /locus_tag="ACHJWA_004263"
     CDS             complement(4576..4989)
                     /gene="hiuH"
                     /locus_tag="ACHJWA_004263"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFO4310741.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNKHYHVPLLLSQYGYSTYRGS"
     gene            5122..5793
                     /gene="hprR"
                     /locus_tag="ACHJWA_004264"
     CDS             5122..5793
                     /gene="hprR"
                     /locus_tag="ACHJWA_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFO4310742.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            5793..7151
                     /gene="hprS"
                     /locus_tag="ACHJWA_004265"
     CDS             5793..7151
                     /gene="hprS"
                     /locus_tag="ACHJWA_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFO4310743.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDLLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNSYLNIDIASPGAK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRITLPQ
                     RN"
     gene            complement(7259..8110)
                     /gene="hchA"
                     /locus_tag="ACHJWA_004266"
     CDS             complement(7259..8110)
                     /gene="hchA"
                     /locus_tag="ACHJWA_004266"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFO4310744.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPQKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKLLTGDSPFAANALGKLAAQE
                     MLAAYAG"
     gene            complement(8702..>9201)
                     /locus_tag="ACHJWA_004267"
     CDS             complement(8702..>9201)
                     /locus_tag="ACHJWA_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFO4310745.1"
                     /translation="VHKGTHNTRYGDRFDATAGGETAVAWTVGAKYDANNVYLAAMYA
                     EPRNMTGYGDADAIANKTQNFEVVAQYQFDFGLRPSIAYLQSKGKDLGGVNSDNFDSQ
                     GNHHYTNKDLVKYVDIGMTYYFNKNMSTYVDYKINLLDNDDDFYKENGIATDDIVAVG
                     LVYQF"
BASE COUNT         2548 a         1857 c         2141 g         2655 t
ORIGIN      
        1 ttgtcaagtg attatatgag aaagacttgg tttattgttg attaatggta tgacctccga
       61 ttttcattga tagcttatgt gaaattcata ttgtgcttga ctttcgaagt gattgagctc
      121 agtgacgaga tttattggaa ttatagttaa acagaagaga ggttaaatac cgtggagagc
      181 tcgagttata caaggcgtta acgtgagcaa taaataggat gcgaagtaat tttggtggct
      241 cggaaaaggc gttttctgtc gttatcaatt gttcgggtgc agttttattt ttaacctgtt
      301 tgcttggaat attctgaggt tgtttgatgc taaccatctg atttttcaat cctaaatata
      361 gaatatttat tccaaataaa acacataata tattgatttt attaacccat agcataggtc
      421 tcgaaaaccg gagtaggggc aactctaccg ggggttcaaa tccccctctc tccgccactt
      481 tatcaatgac ttatctcccg acttcccgcc ttgcttttcc taaacagaac aatcgtagaa
      541 tattcttgaa gggttagatc gtcactgttt tctgttcgat actgtgacat tcagcacttg
      601 attcgctatg gatctgacag gaagctttcg agcgaaaatg tgcagttatt cagtcgtttt
      661 cttatcgctc accattattc ttttagacat tcatcctatg aaactgccgc aaagttggtg
      721 gtgggaactg aagttgcgta gagaaggggt caagaattga gtcaaaaaag gaacgcggcc
      781 gtcagattaa agccgcggga caaagtccat aatttccaag agttgttgaa tttcccagct
      841 tttttgaaag cctttgtatc atactaacaa gtaaaaaagg tttaaatacc aaaattaacc
      901 tgtatttttg ataagtctta ccattgtacg atttacacat tttctgctcc tccttgttga
      961 tagtggaaag agtgcggtat gcacttgcgt aaacatttat gatgcgttga tgtccatggt
     1021 tcacatttcc tttgtcagag gatcgactta tttgcggcga ggcatcattc gtaacagagt
     1081 attatctttc tgaatgtagt gatgaaagag tgctgcgcca gcgtgagcta cgattaaaaa
     1141 gtagcctata tttgcccagg tttcgtgaat attttttatc agtgctttaa tttcgctgtt
     1201 tggggtaaca aagggagaca cattgaaacc aaggaaactc cacgattttc cactgtaagc
     1261 catcaaagca atccccagca gaggaagcgc aaggaaggtt atataaagca tgatgtgcat
     1321 tagcgaagcc gctttcattt gccaggctgg tggcggtggc acaatagaag gatcactata
     1381 acggtgtttt ataatcaggc gtgaaaacat taacacccaa acgaatatac ccgcattgta
     1441 atgtgtttct cgcatcagca gataagtact actaccttta ggaaaccagc cacggagttc
     1501 catcgcggca taggttattg caacgaaaag cagcgttatc caatgtaaat aaatttgagt
     1561 ttttgagaat cggttcattt ttgtagctcc tgtaattttc acagtcaagc tatcccacga
     1621 accttaaaaa ttccttatac agatgtggtt ttaagaattt gttatttaaa aaattgatag
     1681 tgcacattgt aaccagccaa tataagcaat ggcgtgagca catgtatctt tctctgttgg
     1741 ccgtattgtg tatggaatcc gttattggtt aactcaatgc gggttagctt attaatatac
     1801 agacacgtca tagtgatgag cgtgtctgta gctagcttgc gttcagtggc gtttgtggat
     1861 ggaccagtgg cagttatgat gacatactaa agcggcatcg aggcgttatc atgagaatac
     1921 tcaatgagac atcatttcct cgaccacttc ttcgctactc aactgatatg gataatacgt
     1981 tggccagttt tccatttcat ttaataaaga ttgctgtgaa tcattaccca taaaaatgtg
     2041 aaaatgagat gattttcgtg gtgcaattat atgatcgcta aattgtatat atttaggggc
     2101 tttgctttca ggatctttac attcgaataa gtaacgaacg cctttcttgc ctgatttata
     2161 ggtgagtatt ttgtatccat cgtaatcata tttacaggat gttgtttcat tatttctatg
     2221 gaattcaaca atgccgtcct caatgccaat catctcgata tctgttgcat agcctttgtg
     2281 ataataatct ttaatttcag caaatgtttt agttttatct gcatccgctt tcttctgaaa
     2341 gacgggggca agtttgccac tttgcagtaa aggataaacg gattgccaga ctccatccca
     2401 gtcactgagc gttcggtttt gtacattggc atcatcaaaa acaccattag cagctttttg
     2461 ttcgacctct gttaagggtt tgccgtgtga gtgatgacca tgcgaaaagg caggagcgct
     2521 aacaataaag acacctaaag caacagccag tttgtgaaga cgaatcgcca aaacagtttc
     2581 ctccagagtc attgtatatg atgtgtaatg ttatattgta acatatgaca aaatgcaaca
     2641 actcttagga gatgagagca tagttttctc aggtgtactt atctatctta ttgaaataaa
     2701 atgaattatg ttgataattt gaggttgatg acaaacgata cataacctga tgtgatctgc
     2761 gtaggccgga tagggcgctc gcgccacatc cggcagggat aacgcgtact ttgtcggcaa
     2821 tatgaagtta tacagataat ttgttgtgaa cctgcttgcg taaccggtta aaaagggaac
     2881 gcgacttctt ataccgtaag gctaaaagca gtacagccag cccagcgtag atgagcggct
     2941 gcggtgaaat aatcttcact gaccacagat aatgtattgg ggccaggatc gcgacaagat
     3001 agacgaaatt atgcaactgt tgccagtgct tgccgagttt tcgctgcatc gcctgggttg
     3061 aagtaaacgc taaagcaagc aaaataatcc agctgataat tcctaacgtt aaataaggcc
     3121 gggtaattaa ctccttgcct aataacgcca gattattcac gcccagctcc agcaatgcgt
     3181 aactggttaa atgcagtgtc gcccaggcaa agcaccataa gcctaacagg cggcgtgtgc
     3241 gtatcaataa cggctgtttt gcgtagcgtg ccagaggtgt gattaacaag gtcgccagca
     3301 aaaatttcag tgcagtgcga ccggtaaaat gctgaatatc tttcaccgga tcggcaccca
     3361 gtccaccgtg attgatcgcc cagaccagcc agagaaacgg caacaatccg gcaagatgca
     3421 ggcaaacttt cagccatgtc acctgttttg ctgtcagacg cattcagaaa ttctcccgca
     3481 aatccaggcc acgatacagc gatgccacct ggtcggcgta accattaaac agtagcgttg
     3541 gctggcgctg tacatcgagg atgccgcctg aaccaataaa tcgttcggta gcctgtgacc
     3601 agcgtgggtg atcaacatgc ggattaacgt tggcgtaaaa accgtattcg tcaggcgctg
     3661 ccagattcca ggtggttggc ggacgctcgc gggtcagctt aatactgacg atcgatttaa
     3721 tccctttaaa gccatatttc cacggcacaa tcagtcgcac cggcgcgcca ttttgtggcg
     3781 gtaacgcctt gccataaaca cctacggtca tcagtgtgag cggatgcatt gcttcgtcga
     3841 gacgcaatcc ttcgacataa ggatatttca gcccgccgcc gataaagcgg tcctgctggc
     3901 ctggcatctg ttccggtgca taaattgttt cgaaagcgac atacttcgca ttgctggtgg
     3961 gttcggcaag cgccagcaat ttgtgcagcg gaaaaccaat ccacggcacc accatcgacc
     4021 acgcttccac gcagcgcata cgataaatac gctcttccag cgggaagcga cgggttaaat
     4081 catcgtgatc gagggtcaat ggttttgcca cttcgccgct gattttcagt gtccatggat
     4141 cggttttcag gctaccagca ttagcggcgg gatcggcttt atccagcccg aattcataga
     4201 agttgttata accggagact ttatctgctg gcgtcagtgg caggttattt tgccaggcgg
     4261 caggcttgct gaactccagc gcttttccgg cgggggcggg tgggcgatcg ttccctttaa
     4321 accagctaag cagatcggca tgcgcagcgt gaggcaaaga aagtgcagct gcgctgatgc
     4381 ccagtgcttt taacacctgc cgacgcttca taaagaatac cgactcggcc gtaacatctg
     4441 attcttttaa aaattgattc tttttcataa gccatcacac tttttgtcgt tagccagcaa
     4501 gagccgttta ctgttaatct gtcagggagt gtcctcccta acagtagcag agatctgctt
     4561 ttacatattt atgcattaac tgccacgata ggttgaatac ccatattggc taagtaataa
     4621 aggcacatga taatgcttgt tcactttatt aatatgaaat tcaacgggga tctcagggaa
     4681 gaaactttca agattttgtt tcttgaaata gtccccggtt ttaaatacga cacggtaatc
     4741 gcccgtagtt gcagtttgct cgggccacag tgccttaatt cgtccatcct tatctgtttt
     4801 ggcggtatta agttgtaacc agccgttgtc cgctttcttt tcaagagtga ctgtcacgtc
     4861 ggcagcgggt tttcctgttt gctggttcaa aatgtgcacg ctaagaatgt tttgttgtgc
     4921 agcatagacc aaagaaggta atgaaaatgc agccgttact acggagagta ctaaataacg
     4981 ctttaacatg tttatatcct tgtcatgtga atgagttttg aggatagcga aaaagcgtcc
     5041 agggtaacct tacagcagca ttacaatttt gtaatgaacc aggctgtttc tatgacatat
     5101 gatttatggc atattatttt catgaagatt ttacttattg aagataatca aaggacccag
     5161 gagtgggtaa cgcaggggct ttccgaagcg ggttatgtca ttgatgccgt ttctgatggc
     5221 agagatgggc tttatcttgc gctgaaggat gattatgcat tgatcattct ggatattatg
     5281 cttccgggta tggatggctg gcagatctta caaacgttaa gaacggcaaa gcaaacccct
     5341 gttatttgcc ttactgcaag ggattctgtc gatgacagag tcagagggct ggacagtgga
     5401 gcaaatgatt atctggtaaa acctttttca ttttctgagt tgctggcaag ggttcgggca
     5461 caattaaggc aacatcacgc tttgaattca acattagaaa tcagcggctt aagaatggac
     5521 tctgttagtc aaagtgtgag cagggacaat atcagtatta cactgacgcg caaggagttt
     5581 cagttacttt ggctactggc ctccagagct ggcgaaatta tacccagaac ggttattgcg
     5641 agtgaaattt ggggaatcaa ctttgatagt gataccaata cggtggatgt cgccattcgc
     5701 aggctccgcg caaaagttga tgatcctttt cctgaaaagc taattgccac aatccgtggg
     5761 atgggatatt cgttcgtagc ggtaaaaaaa taatgaaaag attatctata accgtccgtt
     5821 taaccttgct ttttatattg ctgctgtctg ttgctggcgc cggaattgtc tggactctct
     5881 ataatggcct cgcaagtgag ttgaaatggc gcgatgatac aacactcatt aaccggacag
     5941 cgcagatcaa gcagttatta attgatgggg taaatccaga tacgttacct gtatacttta
     6001 accggatgat ggatgttagt caggatatct tgatcattca tggtgatggc atcaataaaa
     6061 ttgttaaccg gacaaatgtc agtgatgaca tgttaaataa catacctgct agtgagacaa
     6121 tcagcgcagc tggcatttac agaagcatta ttaatgatac agagatagat gctttacgaa
     6181 ttaatattga tgaagtttcg ccatcattaa cggttactgt ggctaaattg gcttcagcca
     6241 gacataacat gcttgaacag tataaaatca atagcattat aatttgcatt gtcgccatta
     6301 tactgtgctc agtattaagt ccgctgttaa tccgaacggg gttacgagag atcaaaaagt
     6361 tgagtggtgt aacggaagcg ctgaattata acgatagccg ggagcctgtt gaggttagcg
     6421 cattaccgag agaactaaaa cctcttgggc aggcgttgaa taaaatgcat catgctttag
     6481 tcaaagattt tgagcgtcta agtcagtttg ctgacgatct cgctcatgaa cttagaacgc
     6541 caattaatgc attactgggt cagaatcagg ttacgctcag tcaaaccaga agtatcgctg
     6601 aatatcaaaa aacaattgcc ggtaacattg aagagctgga aaatatttcg cggttaacag
     6661 agaacatact gtttcttgcc agggcagata aaaacaatgt tttggtgaaa ctggacttgc
     6721 tttctctcaa taaggaagtc gaaaatttgt tggactatct tgaatatctt tcagacgaga
     6781 aagagatttg ctttaaggtc gagtgcaatc agcaaatctt tgcggataaa attttactgc
     6841 aacgaatgtt atcgaatctt attgttaacg ctattcgata ttcgccagaa aaatcgcgta
     6901 ttcatataac cagttttctt gataccaaca gctatcttaa tattgatatc gccagccctg
     6961 gagcgaaaat taatgagcct gaaaaactct tccgtagatt ttggcgggga gataattcgc
     7021 gtcattccgt aggtcaggga cttggtcttt ctttagtcaa agcgattgcc gaattgcatg
     7081 ggggaagtgc tacgtatcac tatctcaata agcataatgt gttccggatt acgttgccgc
     7141 aaagaaatta acatagggct tcagtacgcc ataaattaac cgtgaaaaaa cgcagccacg
     7201 tcgtgagtac taacgcggct gcgattgatt atgcgcttac attcaaacgt aacagggatt
     7261 aacccgcgta agctgccagc atttcctgcg ccgccagttt acccaacgca ttcgctgcaa
     7321 aaggactgtc gccggtgaga agtttacggt ccttatgtac tcgcccggtg atgtcgtcat
     7381 taatgatgtt catgcccatt ttcttcagtt cttcgccgaa gtaccaggtg agatgacccg
     7441 gcatatagcc aatctctggc gtttgtttgt ctgcggcgtc tgggaatgcg caaatggaat
     7501 aaccattcag tggattatcg ccgtggcgaa gcgccagaaa agccgccggg ccgtggcaaa
     7561 gggagataac aaaacggtca tttttgattg cccactgtaa agcggcagct acgtcctggc
     7621 tttcaggtaa accaataagt gcgccatgac caccaggaac aaagattgct gcatattcgc
     7681 tatcagcgtt gaggctggcg acaacatccg cgagtttctt cggattgcgg aacaacgatt
     7741 tatgctgctc aaagaatggc attacttttt catctttctg cggcatagcc cagtattcaa
     7801 acttggtcat cagaccggaa atggtcgcca cttcgaattc gaaacctgca gcatggagat
     7861 gatacaacgg cagcaacgtt tcaatcggat ggttaccggt cgagaacagt tttccgttat
     7921 cggtcggcaa ataacgttcg tccgcggcga tcaccagaat tttatgttta ccgcgataag
     7981 gttttggata gtccacgcca tcaagatcag agacaggact ggtatattgg ctaagcgaat
     8041 attctgaagg gaagaatgca ttatcttcag caatatcgac ctgcggattt ttactggttt
     8101 gaacagtcat agtgtattcc ttattgttgc ttagttaggg tagtcactat atttgcgact
     8161 gagctggtcg ctatttttaa ccaggctgga aaattgagtc aatatatcga aagcggtagt
     8221 cggcgggtgt aagtatgtaa tgatgatgct gcactggtgt tgaatattat tcacgccaaa
     8281 ttttccttat tgagcaactt cgtcgcaagt ggcctcaatg attttagacc attatcagag
     8341 gtacggggcg gcggggagag atttagtgct gtggaatatt gtggtgctga tgaatattta
     8401 tatcgcggat tggtagagaa aaagcttatt tcatgcacta tttttgcaaa tatttctata
     8461 tgggaagcgt gtttgttacg gattaattca taaagaaatg catttgggtg ttggcttgaa
     8521 gagtcgctgg ttgatgttat tgtgccagga ggggcagggt aagggggtgt atcgataagc
     8581 tttggcaatt gcgtgtgccg cagatggaga tgaaatgcca ataaaaaagc ctgagttcga
     8641 tgagggacga actcaggcta gtgttcagtt ttacaataac acgccgcggc gcgttggctg
     8701 attagaactg gtagactaag cccacagcca cgatatcatc ggtagcgatg ccgttttctt
     8761 tgtagaagtc gtcgtcgttg tccagcaggt tgattttgta atcaacataa gtggacatgt
     8821 ttttgttgaa gtagtaggtc ataccaatat caacatattt aaccagatct ttgttggtgt
     8881 aatgatgatt accctggctg tcgaagttat cactatttac accgcctaaa tccttacctt
     8941 tagattgcag ataagcgatg gacgggcgca gaccgaagtc gaactgatac tgagcaacaa
     9001 cttcaaagtt ttgggttttg ttagcgatgg catcagcatc accgtaacca gtcatgttac
     9061 ggggttcagc atacatagct gctaagtaaa cgttattagc gtcgtattta gcaccaacgg
     9121 tccatgcaac tgctgtttca ccgccagcag ttgcatcgaa gcgatctcca taacgagtgt
     9181 tatgagtacc tttgtgaacc t
//
LOCUS       DAZFAB010000147         9161 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZFAB010000147 DAZFAB010000000
VERSION     DAZFAB010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9161
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.147"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..234
                     /locus_tag="ACHJWA_004268"
     CDS             <1..234
                     /locus_tag="ACHJWA_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO4310746.1"
                     /translation="GKLIDTGFCIFALSKLAMALSSTLDSIPLSMQRQFPDLTPRHLD
                     HLKTLIAKGANQCARAGDKLPDLLDEYIRATTE"
     gene            206..2135
                     /locus_tag="ACHJWA_004269"
                     /pseudo
     CDS             206..2135
                     /locus_tag="ACHJWA_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
     gene            2119..2325
                     /locus_tag="ACHJWA_004270"
     CDS             2119..2325
                     /locus_tag="ACHJWA_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFO4310747.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLDELN
                     RAINDAESVLGTTRRRRRPLGVRL"
     gene            2322..3914
                     /locus_tag="ACHJWA_004271"
     CDS             2322..3914
                     /locus_tag="ACHJWA_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4310748.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYNGGGAGFGGQMAEWLPPAQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMSGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQPVWDTETTQ
                     LFRTRFKAVSPKRVDTPGHGMGNRFLRAGVEVDRYGRAVAYHICEDDFPRSGSGRWER
                     IPRELPTGRPAMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKAMYA
                     ATIESDLDTEKAFEYIAGAPQGQKDNPLINILEKFSSWYDTNNVTLGGVKIPHLFPGD
                     DLKLQTAQDSDNGFSALEQALLRYIAAGLGVSYEQLSRDYSKVSYSSARASANESWRY
                     FMGRRKFIAARLATQMFSYWLEEALLRGIIRPPRARFDFYQARSAWSRAEWIGAGRMA
                     IDGLKEVQESVMRIEAGLSTYEKEPALMGEDYQDIFRQQVRESAERQKAGLSRPVWIA
                     QAYQQQIAESRRPEEETTPRET"
     gene            3904..5409
                     /locus_tag="ACHJWA_004272"
     CDS             3904..5409
                     /locus_tag="ACHJWA_004272"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO4310749.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADSQVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMSHVSYAGHLAQAGVDITLIYSGAHKVDGN
                     QFEALPAEVRQNMQQRIDAARRMFAEKVAMFTGLSVDAVTGTEAAVFEGQSGIDAGLA
                     DELVNASDAISVMATALNSNVRGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAAAAPQQPVASTQSEADRIMACEEANGREQLAATLAAMPE
                     MTVEKARPILAASPQADAGPSLRDQIMALDEAKGAEAQAEQLAACPGMTVESARAVLA
                     AGSGKAEPVSASTTALFERIMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            5446..5793
                     /locus_tag="ACHJWA_004273"
     CDS             5446..5793
                     /locus_tag="ACHJWA_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO4310750.1"
                     /translation="MVTKNITEQRAEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     EASGKLVVWDGQKAGSAVGILVLPLEGTETVLTYYKSGTFATEAIRWPESVDEHKKAN
                     AFAGSALSHAALP"
     gene            5851..6102
                     /locus_tag="ACHJWA_004274"
                     /pseudo
     CDS             5851..6102
                     /locus_tag="ACHJWA_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
     gene            6099..6839
                     /locus_tag="ACHJWA_004275"
     CDS             6099..6839
                     /locus_tag="ACHJWA_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4310751.1"
                     /translation="MPLEPVKGAGTTLWVYNGQGDAYANPLSDDDWQRLAKVKDLTPG
                     EMTAESYDDNYLDDEDADWTATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFESGDVR
                     AYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSKITPVSAI
                     KVTPTSGTVAKGKTTTLTVSFEPESATDKTFRAVSADPSKATISVKDMTITVNGVATG
                     KVQIPVVSGNGQFAAVAEVTVTEAGAAG"
     gene            6853..7284
                     /locus_tag="ACHJWA_004276"
     CDS             6853..7284
                     /locus_tag="ACHJWA_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO4310752.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEQAESSGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTQSPSMNEAVMKIEQEVLTTWPADAIARAED
                     VVLCLSGMSGAVRPDTDITEVAKNNTLTDDDFSAGKSSTAS"
     gene            7311..7724
                     /locus_tag="ACHJWA_004277"
     CDS             7311..7724
                     /locus_tag="ACHJWA_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO4310753.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWHRFYRTHYFQDTQLDMHFSGLTY
                     AVLSLFFCDPDMHPSDFSLLVPRHEEEQVERPDEDKMLMQKAAGLAGGVRFGGDGGRD
                     ILSSADVADVMVDDAALMMASAGIPGGVRYVPAGW"
     gene            7705..>9161
                     /locus_tag="ACHJWA_004278"
     CDS             7705..>9161
                     /locus_tag="ACHJWA_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFO4310754.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMSRVRRHFSGLDTDVRKTASAV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGVTSLAVATGALVYAWYQGDSTLSAFNKTLVLSGNQS
                     GLTADRMLTLSRAGQAAGLTFNQARESLAALVNAGVRGGEQFDAINQSVARFASASGV
                     EVDKVAEAFGKLTTDPTSGLIAMVRQFRNVTAEQIAYVAQLQRSGDEAGALQAANDIA
                     TKGFDEQTRRLKENMGTLETWADKTGKAFKSMWDAILDIGRPESSADMLASAQKAFDE
                     ADKKWQWYQSRSQRRGKTASFRANLQGAWNDRENARLGLAAATLQSDMEKAGELAARD
                     RAERDASQLKYTGEAQKAYERLLTPLEKYTARQEELNKALKDGKILRADYNTLMAAAK
                     KDYESTLKKPKSSGVKVSAGERQEDQAHAALLALET"
BASE COUNT         2094 a         2243 c         2897 g         1927 t
ORIGIN      
        1 ggaaaactga ttgacaccgg gttctgtatt tttgccctca gtaagctggc aatggcgtta
       61 tccagtacgc ttgattccat ccctttatcc atgcagcgac agtttcctga tttaacaccg
      121 cgccatcttg accatctgaa aacccttatt gctaaggggg caaatcagtg tgcgcgggca
      181 ggggataaat taccggattt acttgatgaa tatatcagag caacaactga ataatatgat
      241 gagcgctgtc acaactgcat tacagcccct gataagggca ttgccggtga cgccagttga
      301 atgggctgat caaaattatt atctgcctaa agaatcttca tatggtgagg gcgaatggaa
      361 aacgctgcca ttccagatcg ccatcatgaa cagtatgggg aatgatcaga tccgcactgt
      421 taatctgatt aaatctgccc gtgttggcta tacaaagatg ttgctggggg gggggcgggt
      481 attttattga gcataaatcc cgaaacagtc tgctttttca gcccacggat tctgccgctg
      541 aagattttat gaagtctcac gtggaggcga cgattcggaa cgtgccatgc ctgaaagacc
      601 tttccccatg gctgggtcgt aaacatcgtg acaatactct cacgctgaaa cgcttttcat
      661 cgggcgtcgg tttctggtgc ctgggcggcg ctgccgccaa aaactaccgt gaaaaatccg
      721 ttgacgtggt ctgctatgac gaactttcct cgttcgagcc ggatgtcgaa aaagagggct
      781 cgccaaccct gctgggggat aagcgtattg aggggtcggt gtggccaaaa tccattcgcg
      841 gctcgacgcc taaaatcaaa ggcacctgcc agatcgaaaa agccgctaac gagtcggcgc
      901 attttatgcg tttttatgtg ccctgcccgc actgtgggga ggcgcagtat ctgaaatttg
      961 gcgatgagtc cacgcctttt gggcttaaat gggagaagga cagccctgaa agtgttttct
     1021 acctctgtga acatcatggc tgcgtgatcc atcagtctga actggaccag agcaacgggc
     1081 ggtggatctg tgaaaacacg ggcatgtgga cccgtgacgg tctgacgttt ttcagcgccc
     1141 ggggtgatga aattccgccg ccgcgctcca tcatgttcca tatctggacg gcgtacagtc
     1201 cgttcaccac ctgggtacag attgtctatg actggctgga tgcactgaaa gatcccaacg
     1261 gcctgaaaac ctttgtgaac accacgctgg gcgagacctg ggaagaggcc gtgggcgaaa
     1321 aactcgatca ccaggtactg atggataagg tggtgcgtta cacggcggcg gtgcctgccc
     1381 gggtggttta tctgacggcg ggcattgact cgcagcgaaa ccgttttgag atgtatgtct
     1441 ggggatgggc tccgggagag gaagcctttc tggtggataa aatcatcatt atggggcgtc
     1501 ctgatgagga agagacgctg ttacgtgtgg atgcggcgat caacaaaaaa taccgccatg
     1561 cggatggcac cgaaatgact atttcccgtg tctgctggga caccgggggg atcgatggtg
     1621 aaattgttta tcagagatca aaaaaacacg gtgttttccg ggtgctgccg gtaaaaggcg
     1681 catctgtcta tggcaagccg gtgatcacca tgccaaaaac ccgcaatcag cggggcgtgt
     1741 atctgtgtga agtgggaacg gacaccgcaa aagaaattct ctatgcccgt atgaaagccg
     1801 atccctcgcc tgcggatgaa gccacgtcgt atgccatccg ttttcctgat gatccggaga
     1861 ttttttcgca gacagaggcg cagcaactgg tggcggaaga gctggtggag aagtgggaaa
     1921 aaggaaagat gcgtctgctg tgggataaca aaaagcggcg taacgaagcg ctggactgcc
     1981 tggtgtatgc ctacgcggca ttacgtgtgt ccgtgcaacg ctggcagctt gatctggctg
     2041 tactggcaaa atcccgggaa gaagagacga cccggccaac cctgaaagaa ctggcagcga
     2101 agctgtccgg aggagtgaat ggttacagtc gctgaactgc aggcgctgcg tcaggcgcgc
     2161 cttgatttat taaccggtaa acgggtggtg tctgtccaga aagatggtcg cagaattgaa
     2221 tatacggcgg cttctctgga tgagcttaac cgggcgatca atgatgcgga gtcggtactg
     2281 gggacaaccc ggcgtcgccg tcgtccgctg ggagtgaggt tatgaaacga acgcctgtcc
     2341 tgattgatgt gaacggcgtt ccgcttcgtg agagtctcag ctacaacggg ggcggtgcag
     2401 gatttggcgg gcaaatggcg gagtggttgc caccggcgca gagtgccgat gcggccctgc
     2461 tgcccgcgtt gcgtctgggg aatgcccggg cagatgatct ggtgcgcaat aacggaatag
     2521 cggccaatgc ggtggcactg cataaggatc acattgtcgg gcatatgttt cttatcagct
     2581 accgtccgaa ctggcgctgg ctggggatgc gggagaccgc agcaaaaagc tttgtcgatg
     2641 aggtggaggc ggcctggtcg gaatacgccg aagggatgtc tggcgagatc gacgtggaag
     2701 gaaaacgcac gttcacggaa tttatccgtg aaggtgtggg cgttcatgcg tttaacggcg
     2761 aaatctttgt gcagccggtc tgggatacgg aaaccacgca gttattccgt acgcgtttta
     2821 aagccgtgag tccgaaacgg gtggacacgc caggacacgg tatggggaac cgttttctgc
     2881 gggccggggt ggaggtcgat cgatatggcc gtgccgttgc gtaccatatc tgtgaggatg
     2941 attttcctcg ctccgggagt ggacgatggg aacggatccc gcgtgaactt cccaccgggc
     3001 gtccggccat gctgcatatt ttcgagccgg tggaggacgg gcagacccgt ggggccaacc
     3061 agttttacag cgtcatggaa cggctgaaga tgctcgattc cctgcaggca acacagcttc
     3121 agtcggccat tgtgaaagcc atgtatgcag cgacgattga aagtgacctt gataccgaaa
     3181 aggcctttga atatatcgcc ggtgcgccgc aggggcagaa ggataatccg cttattaata
     3241 ttctggagaa gttctccagc tggtatgaca cgaataacgt gacgctgggt ggtgtcaaaa
     3301 ttccgcacct tttccccggg gatgatctga aactacagac tgcgcaggat tcagacaatg
     3361 gattttcggc gcttgaacag gcgctgctgc ggtatatcgc cgccggtctt ggcgtttcct
     3421 acgaacagtt gtcccgtgat tactcgaagg tcagttattc aagtgccagg gcctctgcca
     3481 atgagtcgtg gcgctatttt atggggcggc gaaaatttat tgcggcccgg ctggccacgc
     3541 agatgttttc ctactggctg gaagaggcac ttcttcgggg gattatccgt ccgccacggg
     3601 cgcgttttga tttttatcag gcgcgatcag cctggtcacg ggcagagtgg attggtgccg
     3661 gaagaatggc cattgacggg ctcaaggagg ttcaggaatc ggtgatgcgc attgaggccg
     3721 gactgagcac gtatgagaaa gagccggcgc tgatgggcga ggattatcag gacattttcc
     3781 gccagcaggt cagggaatct gcagagcggc aaaaagccgg actctcacgt ccggtgtgga
     3841 tagcgcaggc gtatcagcag cagatagcgg agagtcgcag gccggaagag gagacaacac
     3901 cccgtgagac gtaatctttc acacattatt gccgcagcat tcaatgaacc gctgcttctg
     3961 gagcccgcct atgcgcgggt tttcttttgc gcgctcgggc gcgagatggg ggcagcaagt
     4021 ctttcggtac cacagcagca ggtacagctt gatgctcccg gaatgctggc tgaaacggac
     4081 gagtacatgg ccggaggtaa acgaccggcc cgtgtttacc gggtggtgaa cggtattgct
     4141 gtactgccgg tgaccggcac gctggtgcac cggctggggg gtatgcggcc attttccgga
     4201 atgacaggct atgacggcat tgtcgcctgt cttcagcagg caatggcgga tagccaggtg
     4261 cggggcgtac tgctggacat tgacagtccg ggcgggcagg ccgccggcgc gtttgactgc
     4321 gctgacatga tttaccgcct ccgtcagcag aagccggtct gggcactgtg caatgacacg
     4381 gcctgttctg cagccatgct gctggcgtcg gcctgctccc gacggctggt tacccagaca
     4441 tcccgtatcg gctccattgg cgtgatgatg agccatgtca gctatgccgg tcatctggcg
     4501 caggccggtg tggatatcac gctgatttac tcaggggcgc acaaggtgga tggcaatcag
     4561 tttgaagcct taccggcaga ggttcgccag aacatgcagc agcgcattga tgcggcgcgc
     4621 cggatgtttg ccgaaaaagt ggccatgttt accggtctgt ctgttgatgc cgtcacggga
     4681 acagaggccg ccgtttttga aggtcagtcc ggcattgatg ccgggctggc ggatgaatta
     4741 gtcaatgcgt cggatgccat cagtgtgatg gccacggcgc tgaacagtaa tgtcagagga
     4801 ggcactatgc cgcaattaac tgcaacggaa gccgccgcgc aggagaacca gcgagtgatg
     4861 gggatcctga catgccagga agcgaaagga cgtgaacagc ttgccacgat gctggcagga
     4921 caacagggca tgagcgttga acaggcccgg gcgattctgg ccgcggcggc accgcagcag
     4981 ccggtggcat ccacgcagag tgaagccgat cgcattatgg cgtgtgaaga agcgaacggt
     5041 cgtgaacaac tggcggcaac gctggcggcg atgccggaga tgacggtgga aaaagcccgc
     5101 ccgatcctgg ctgcttcacc gcaggcggat gccggaccct cactccgtga tcagatcatg
     5161 gcactggatg aggcaaaagg ggctgaggcg caggctgaac agctggctgc ctgcccggga
     5221 atgactgtgg agagcgcccg ggctgtgctg gctgcgggat caggtaaggc agaaccggtc
     5281 tctgcatcca caaccgccct gtttgaacgc atcatggcga accattcacc ggctgcggta
     5341 cagggtggcg tgccacagac gtcagcagac ggtgatgcgg acgtgaaaat gctcatggcc
     5401 atgccatgaa gtcagtgctg accatcaaca ggaggttttt acaatatggt aacgaaaaac
     5461 atcactgaac agcgtgcgga agtacgtatt tttgccggta atgatccggc tcataccgcc
     5521 acaggcagca gcgggatttc ctcggcaaca ccggcactga cgcccctgat gctggatgaa
     5581 gccagcggga aactggtggt ctgggacgga cagaaagccg gtagtgcagt tggcatactg
     5641 gtactgccgc ttgaaggcac agagacggta ctgacctatt acaagtcggg gacctttgcg
     5701 acggaggcaa tccgctggcc tgaaagtgtg gatgaacaca aaaaggcaaa tgcctttgcc
     5761 ggcagtgccc tgagtcacgc ggcgctgccg taacacgtta tcaggccacc gcggtggcct
     5821 gactgatttc tgaatgaaag gaactgattt atgggattgt ttacgacccg ccagttactc
     5881 ggttataccg aacaaaaagt gaaatttcgt gcgctgtttc tggagctgtt tttccgccgt
     5941 acggtgaatt tccataccga agaggtgatg ctggacaaaa ttaccggaaa aacgccggtg
     6001 gcggcctatg tctccccggt tgttgaagga aaagtgctgc gtcatcgcgg tggtgaaacc
     6061 cgcgtgttac gtccgggcta cgtcaagccg aaacacgaat tcccctggag ccggtaaaag
     6121 gagccggtac caccctgtgg gtttataacg gtcagggtga cgcctatgca aacccgttgt
     6181 cagacgatga ctggcagcga ctggctaagg tgaaggatct gacgccgggc gagatgacgg
     6241 cagaatccta cgatgataac tacctggatg atgaagacgc ggactggacc gcgaccgggc
     6301 aggggcagaa atctgcaggt gataccagtt ttacgctggc ctggaaaccg ggagaggaag
     6361 gccagaaagg gcttataggc tggtttgaaa gcggcgatgt ccgggcctat aaaatccgtt
     6421 ttccgaatgg cacggtggat gtgtttcgtg gctgggtcag cagtatcggt aaggccgtga
     6481 cggcgaaaga agtgatcacc cgcacggtga aagtcactaa cgtgggtaaa ccttctgtag
     6541 cggaagaacg cagcaaaatt acgccggtca gtgcgattaa ggtgacgccg acatccggta
     6601 cggtggcaaa agggaaaaca accaccctga cggtttcttt tgagccggaa agtgcaacag
     6661 acaagacgtt cagagcggtt tccgccgatc cgtcgaaagc caccattagt gtgaaagata
     6721 tgacaattac ggtaaacggc gtggcgacag gtaaggtgca gatccctgtg gtgagcggaa
     6781 atggtcagtt cgccgcagtg gctgaagtca ccgttactga agcgggcgct gcagggtaaa
     6841 cggaggtcat acatgtttct gaaaacagaa caatttgaat ataacggtgt gtctgtcacg
     6901 ctttccgaat tgtctgcgct gcagcgtatc gagcatcttg ccctcctgaa acggcgtgca
     6961 gaacaggcag aatccagcgg caacctgcag gtaagcgtgg aagatctcgt cagaaccggc
     7021 gcgtttctgg tggcgatgtc cctgtggcat aaccatccac agaaaacgca gtcaccgtca
     7081 atgaatgagg ccgtgatgaa gatagagcag gaagtgctca ccacctggcc tgccgatgcc
     7141 attgcccggg cggaagacgt ggtgttgtgc ctgtccggga tgagcggggc tgttcgtccg
     7201 gatactgata ttactgaagt ggcgaaaaat aacacgctga ctgatgatga tttttctgcg
     7261 ggaaagtctt cgacggcgag ctgaactttg ccctcagact ggcgcgtgag atggggagac
     7321 ccgactggcg cgccatgctt gccgggatga catccaccga atatgccgac tggcaccgtt
     7381 tttaccgcac gcattatttt caggataccc agctggatat gcatttttcc gggctgacgt
     7441 acgctgtact cagcctgttt ttttgcgatc cggatatgca tccctctgat ttcagtctgc
     7501 ttgtcccccg gcatgaggaa gagcaggtgg agaggccgga tgaggacaaa atgctgatgc
     7561 agaaagcggc aggacttgcc ggaggcgtcc ggttcggtgg ggacggaggg cgcgatattt
     7621 tatcgtctgc ggatgtggcg gatgtcatgg tggatgatgc cgcattaatg atggcttcag
     7681 cggggattcc gggaggtgtg agatatgtcc cagccggttg gtgatcttgt tattgacctg
     7741 agtctggatg ctgtccgttt cgatgagcag atgagccggg taaggcgtca tttttcaggt
     7801 ctggataccg acgtcagaaa aaccgccagt gctgttgaac agggcctgag ccgccaggcg
     7861 ctggctgcac aaaaagccgg gatttccgtc gggcagtata aagcggccat gcgaaccctg
     7921 cccgcacagt ttacggatat cgccacgcag cttgccggtg gtcagaatcc ctggctgatc
     7981 ctgctgcaac agggcggtca ggtgaaggac tccttcggcg ggatgatccc catgttcagg
     8041 gggcttgccg gtgcgatcac cctgccgatg gtcggggtca cctcgctggc ggtggcgaca
     8101 ggtgcgctgg tgtacgcctg gtaccaggga gattccacgc tttcagcgtt taataaaacc
     8161 ctggttcttt ccggtaatca gtccggactg actgccgatc gcatgctgac gctctcaaga
     8221 gccgggcagg cagcagggct gacgtttaac caggcgagag agtcactggc agccctggtg
     8281 aatgccggtg tgcgtggtgg tgaacagttt gatgccatca accagagtgt cgcgcgtttt
     8341 gcttctgcat ccggtgtgga ggtggacaag gttgcagagg ctttcggaaa actgaccacc
     8401 gaccctacgt cggggctgat tgcgatggtg cgccagttcc gtaacgtgac ggcagagcag
     8461 attgcgtatg ttgcgcagct gcagcgttcc ggtgatgagg ccggggcctt acaggcggcg
     8521 aacgatatcg ccacgaaagg ctttgatgag cagacccgtc gcctgaaaga aaacatgggg
     8581 acactggaga cctgggcgga taaaaccggg aaggcattca aatcgatgtg ggatgccatt
     8641 ctggatatcg gtcgtcctga gtcctcagcg gatatgctcg ccagtgcaca gaaggcattt
     8701 gatgaggcgg ataaaaaatg gcagtggtac cagagccgga gccagcgccg gggaaaaacc
     8761 gcctctttcc gggccaacct tcagggcgca tggaatgacc gggaaaatgc ccgtctgggg
     8821 ctggcagcgg ccacgctgca gtcggatatg gaaaaagccg gtgaactggc cgccagggac
     8881 cgggccgaac gggacgcatc acagctgaag tataccggag aggcgcagaa ggcgtatgag
     8941 cgtctgctga cgccgctgga gaaatatacc gcccgtcagg aagaactgaa taaggccctg
     9001 aaagacggga aaatcctgcg ggcggattac aacacgctga tggcggcggc gaaaaaggat
     9061 tatgaatcga cgctgaaaaa gccgaagtcg tcaggagtca aagtgtcagc cggtgagcgt
     9121 caggaagacc aggcgcatgc tgccctgctg gcgcttgaaa c
//
LOCUS       DAZFAB010000148         8978 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZFAB010000148 DAZFAB010000000
VERSION     DAZFAB010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8978
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.148"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            126..554
                     /gene="uspC"
                     /locus_tag="ACHJWA_004279"
     CDS             126..554
                     /gene="uspC"
                     /locus_tag="ACHJWA_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFO4310755.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCRKYH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(561..1985)
                     /gene="otsA"
                     /locus_tag="ACHJWA_004280"
     CDS             complement(561..1985)
                     /gene="otsA"
                     /locus_tag="ACHJWA_004280"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFO4310756.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(1960..2760)
                     /gene="otsB"
                     /locus_tag="ACHJWA_004281"
     CDS             complement(1960..2760)
                     /gene="otsB"
                     /locus_tag="ACHJWA_004281"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFO4310757.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDINVQLHTVIAQYPGAELETKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(2927..3913)
                     /gene="araH"
                     /locus_tag="ACHJWA_004282"
     CDS             complement(2927..3913)
                     /gene="araH"
                     /locus_tag="ACHJWA_004282"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFO4310758.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVA
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(3928..5442)
                     /gene="araG"
                     /locus_tag="ACHJWA_004283"
     CDS             complement(3928..5442)
                     /gene="araG"
                     /locus_tag="ACHJWA_004283"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFO4310759.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(5512..6501)
                     /gene="araF"
                     /locus_tag="ACHJWA_004284"
     CDS             complement(5512..6501)
                     /gene="araF"
                     /locus_tag="ACHJWA_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFO4310760.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITSNELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKGVEPTKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            7298..7801
                     /gene="ftnB"
                     /locus_tag="ACHJWA_004285"
     CDS             7298..7801
                     /gene="ftnB"
                     /locus_tag="ACHJWA_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFO4310761.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(7881..8132)
                     /gene="yecJ"
                     /locus_tag="ACHJWA_004286"
     CDS             complement(7881..8132)
                     /gene="yecJ"
                     /locus_tag="ACHJWA_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFO4310762.1"
                     /translation="MSQTLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(8247..8333)
                     /gene="azuC"
                     /locus_tag="ACHJWA_004287"
     CDS             complement(8247..8333)
                     /gene="azuC"
                     /locus_tag="ACHJWA_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFO4310763.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            8595..8918
                     /gene="yecR"
                     /locus_tag="ACHJWA_004288"
     CDS             8595..8918
                     /gene="yecR"
                     /locus_tag="ACHJWA_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFO4310764.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGISDRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
BASE COUNT         2325 a         2273 c         2046 g         2334 t
ORIGIN      
        1 gattaattgt ctttactaat aatcagacta atatttacct gtttgactga gttgggattg
       61 cgtcgtttct ccattaggag taaagcttta atgtcacctg aagttcacag aataaggaac
      121 aggttatgag ctatagcaat attcttgtcg ctgttgcggt tacaccggaa agtcagcaac
      181 tgctggcaaa agcggtatct atcgccaggc cagtaaaggg acacatcagt ttaattactc
      241 tcgcttccga cccggaaatg tacaatcaat tagctgcgcc gatgcttgaa gatttacgca
      301 gcgtgatgca ggaagaaacg caaagctttc ttgataagtt aattcaggat gcggggtatc
      361 ccgttgacaa gacgtttatt gcctacggtg agttaagcga acatattctg gaagtttgtc
      421 gcaaatatca tttcgatttg gtgatttgcg gtaatcacaa tcacagtttc ttttcgcgag
      481 catcctgctc ggcgaaaaga gtgattgcct caagtgaggt cgatgtgctg ttagttccac
      541 ttacgggaga ttaaccgctc ctacgcaagc ttaggaaagg tagcaacttt atcgcgctgc
      601 tggctttccg cgcttcgcgg aactatctgc tttaggtcgc taatgaagca ctcctgccag
      661 tggttaatat cgtttttcac gataacgtcc agcatttctg catgacggga aatacgttcc
      721 gccagcgaca tagtcaatgc acgatccagc gcagctgcaa cttcgtcacg atcgtaggga
      781 ttaacaatta acgccgacgt taactcgttt gccgctcccg caaattgcga aagaacaaga
      841 acgcccgggt tggctgggtc ctgagcggca acatactctt ttgctaccag gttcatcccg
      901 tcacgcagtg gcgtcactaa gcccacgtca gagtagcgga atattttcat cagtaattta
      961 cggtcaaaat gctgattcaa ataataaagc ggcgtccagc ctaattgccc gtatttacca
     1021 ttaattcgtc cggcttcatt ttcgagctga tgacgaatat cctgataggc ttgcacatca
     1081 ccacgcgacg ttggtgcaat ctgggtataa cgaattttac catgatgctg cggatatttt
     1141 tccagcaacg cttcataggc gagaaaacgc tctggcaaac ctttggaata atccagccgt
     1201 tcgacagaaa agatattttg tacgtttttc agttccgctt taagttgcgc cagttttggc
     1261 ggcagtggcc cggcagcctg tttggctatt tcttttggct caatgccaat cgggtaaact
     1321 tctgttcgaa acgctttgcc ccaggctgta tggctttttg cgctacgtgt cgtgacgcgg
     1381 gtcaggttag aaaggcaatc caggaacgcc agacgatcgt tttctgtctg gaaacccagc
     1441 aaatcataat cacaaagctg ttcaagcaag gtgtcatatg tcggcagcgc gttgaagatt
     1501 tccggtgtcg ggaaaggaat atgcagaaag aaaccaatgc gattattcac tccccgtttg
     1561 cgtaattcat gcgcaaatgg caacaggtga taatcgtgga tccagataat gtcatcgtct
     1621 tgcaacagcg gcagtaattt atctgccagc aacgcattta cgcgtagata gccgtcccag
     1681 gcaggacgct gaaattgcac cagatcgagg cgataatgaa aagcaggcca gagaacggca
     1741 ttggagaatt ggttgtagta ttcgtcaagg tcctgttcgc tgaggttaaa agaggcccac
     1801 gtaatgttac cctttttcac cttttttagc ggctgatcct cattccctgt ttcaccactc
     1861 cagccaaacc acagtccgcc tgcggctttc agtgccccca gtatgccaac ggcaaggcca
     1921 ccggcactgg cggcgtgctc atctggtggt gcaatccggt tagatactac gactaaacga
     1981 ctcatagtca tcactcctgt tattttctct tttttgttgt aatgcggtgg ttatcatttc
     2041 aagccagctc cagacatccg gtacgcccgc cagtcgccat gatgcctgag ttgcgcctgt
     2101 gccaattttt actgacattc cgcccagtcg gttaacgact gcgaagccag attcatcggt
     2161 taaatcatcg cccagaaata cgggcgttcg cccgataaag ggagcttcct gcataaaggc
     2221 agcaatcgcc tcacctttac tggtacctct cggtttgatc tcgacaacac actttccctg
     2281 ctgtaacgcc atttgtggcc agatctgagt aatacgttgc gctaatgtca ttaatgcgtc
     2341 ttcatgctgc ggagcctgac gataatgcag cgcaaaagcc atccctttcg tctccagctc
     2401 cgcaccggga tactgggcga tgaccgtatg cagttgcacg ttaatatcac gtgcaatcgc
     2461 atccggcaga tgaacgatat gcgttttacc attgatgtca cggcgctccg ccccatgcac
     2521 gcccgctaac gggaagcgat aaggttttgc cagtgcgtcc agctccacca ttgagcgccc
     2581 tgatatcaat gccaatgcac catcacttgc ggttgccagt agctgaagtc cttgcagaat
     2641 attgtcaggt acgacgacct gatcgggatg cggtttgatt tccgccagcg ttccatcgag
     2701 atcaaaaaac caggcatatt tcgcggatag ttcaggggtt tcggttaacg gttctgtcac
     2761 ccggttctcc tccttctctt ttcttattgt gttgcgcaga acgcgcttta cagacatgta
     2821 agtatagaca gtgtgacggg ggtcgccatt tgcaacgaag aacagccaac cgttgtggcg
     2881 gctggctgtg gtgggaaaaa acgttaaatt gttgtggaaa aaagcatcag acagtgcgtt
     2941 tcgctttttg cttgtaacgg tcgaagatca ccgctgccag caggattaag ccgcgaacca
     3001 cgtactgcgc gaaaggagaa atattaagca ggttcatggc gttttccacg gtgcctaaaa
     3061 ttaagatacc cgccaccaca tatgagattt ttccgatgcc acctttcaga gaaacgccac
     3121 ctaaaacgca ggcggagata acaataagct cataaccaat cgacgtcatt ggctgcccac
     3181 tggtcatacg tgaagccaga ataattccgg ctatcgcaga taccaggcca gagagaacaa
     3241 agataataat tttggtgcga gcaaccggta cacccgccag acgcgcagcc tcttcgttcc
     3301 cgccaatcgc cagggtatta cgaccaaagg tagttttatt cagcagcaaa ccaaagataa
     3361 tcagacacgc gacggtgagc cagattggcg caggcagacc gaaccagttg gcgtaaccaa
     3421 gggcaaagaa gctttcatct tcgataccga ccgctttacc gtcggaaatg atgtacgcca
     3481 gacctcgaac aatctgcatc gttgccagtg tcgtgatcag agcatttatt ttcagtttgg
     3541 cgataacaaa gccattgacc aggccacaga gaatgccaag cagcaacccc gctgccacgc
     3601 caatccacag gctttcagtc agattgataa ccaccgccgt ggtgacaccc gcacaggcaa
     3661 ttacggaggc gacagaaagg tcaaagtcac cggaagcaag gcagaacaac atgccacacg
     3721 ccaccatccc cgacatggaa attgccaggc ccaacccttt catattaata aaggtggcaa
     3781 agttcgggac aaaaatggca caggcgataa aaagcaccgc aaacaccacc agcatgccgt
     3841 actgatccca gatacgcccg aagctgaatg acgacttagg tgcgccagaa cccgatgtag
     3901 aaacagaaga catcatactc tccttactca ggcaactgcc tggctgactt taggcatcgc
     3961 aaggctcagt gcctgacgct catctgcctg ctcgtgtaac aattcaccgg cgatttcacc
     4021 ttcgcgcatc accacaatcc ggtcggcaac gccgaggact tcaggtaagt cgctggaggc
     4081 aaacagcacc gccacaccct gcgccgccag cgcataaatc acgttatata tttcgtgctt
     4141 agcaccaaca tcaatgccgc gcgtaggttc atccagcaaa atgaccttca tctcttccga
     4201 taaccagcgg cccagaatgg ctttttgctg atttccacct gagagattca taatcagttg
     4261 ctcagcgccc ggcgttttga tgttgagcga acgaatgtgg tgatcggcat tgttttcttc
     4321 ccaaccgttg ttgattacac aaccgccgag cacatgttta cgtctggcac tgatgttgat
     4381 attgtcgcga acggagtgca caggaataat gccttccgct ttgcgatctt ccgggcagag
     4441 catcatgcct gcggcaatgg cgtggctcgg tttacgaata tcgatcggct gttggtcgat
     4501 ataaacctga ccggcggtga tttgcgtccc gccaaacatg cctttcatta attcgctacg
     4561 ccccgcccct accagaccaa acagcccaac aatttcacca ctgcgaaccg ccagacttat
     4621 tggcgtacgc acgcctggtg ctttcacagc atcaagacgc aggcgctcct cgccataact
     4681 acgcggttgc cagccgtaga tatcgccaat gtcgcgcccg accatcgcct gcaccagcgc
     4741 gtcgtggtca acctgctgca tatcggtaaa ggttttgaca taacgtccat ctttaaagac
     4801 agtgatggca tcgctgaggg caaatatttc ttccatacgg tgagaaacgt ataagatcac
     4861 ccgcccctct tttcgcagtt cacgaataac gcggaaaaga ttgtcgattt cgcgggcaga
     4921 aagggagctg gttggctcat caaaggcgat aattttagcg ttacgcgcca gcgccttggc
     4981 gatttcaacc atctgccact gaccaatgga gagatatttc agcggcgtgt ccgggtcaat
     5041 atccatacca agatgtttaa gttgtaaacc cgcctcataa ttcagcaatg agcgattcac
     5101 aatgccgcct ttatgcggca gctggccgag atagatgttt tccgcgacgg tcatttccgg
     5161 cacgagatgc agttcctggt aaataatcgc cacacccgcg ttaagtgctg cggtcgtgtc
     5221 ggaaaaggac atttcctgcc cattaatcac tacagaaccc gtggttggcg catagttgcc
     5281 gctgaggatt tttaagagag ttgattttcc tgcgccattt tcacccatca acgcatgaac
     5341 ctgaccggca tagcagtcaa aactaatatc cgtcagcgcc ttaacgccgg gaaacgtttt
     5401 accgatgccg cgaaatgaga gatacggggt agactgttgc ataacgtctc cgtgaatcac
     5461 tggtcgttca caccccctct gcatcatgca aaggggaatt tttccggcaa attacttacc
     5521 gcctaaacct tttttctcca gttcttcttt aaagttgtca cgcgtgatca gcaccacgtc
     5581 ggtaacttcg gtgaatttgg tcggttcaac accttttgct acccagttgt aaagcatttc
     5641 gctggactta taaccatgta cgtccgggct tggcaacagg gaaccgtaga agccggttgc
     5701 ctgtgcctta gacagttcgc tcaccgcatc cacaccgtta ataccgatac cgatgatatc
     5761 ggccgcttta aagccctgac cttccgtcgc gcgtacgccg cccagcacgg tgctgtcgtt
     5821 cataccgacg atcagccaat gtttaacttc cggatgttga accagcattg agttggcagc
     5881 gtcaaatgcc ccggggatgt cgttagattt ggtaggtacc tgataaattt gtttttccgg
     5941 gaatcctgca gctttcagcg catccataga acccgtagta cggcgacgag ccgtatccag
     6001 ttcgttggag gtaatcgcca tcaccgcgct ttctttgaca tcccagccac gtttctgcat
     6061 ctctttatac agttcctggc cctgacgttc gccaattttg gtcgccgcca tcatcaccag
     6121 aggaacggta tccattggct tacctttggc gttaacaaac tggtcatcca cggcaatgac
     6181 tttcatatcg tagccacgcg ctttcgccac gatggcagag ccgagtttcg ggtccggagt
     6241 acaaataacg aaaccttttg cgccactggc ggccaggcta tcgatcgcgt tcaatgtttt
     6301 ttcgccatcc ggcacggcaa tcttaataac ctcaaaccct aaatccttcc cggctttatc
     6361 ggcaaacttc cattcggtct ggaaccacgg ctcttccggt tgcttcacca ggaaaccgag
     6421 cttcaggttc tccgccatag cggattgtga cataacggct gccagaccaa tggctgccag
     6481 ggctttagta aatttgtgca tggttctctc cagctttagt gtcgttttgt gcagggcaaa
     6541 aacgaatgac attcgttaaa ttaatcggaa aacaaagcat taccttttaa ctaaaagata
     6601 agtgactgtg ttgacatagt tttagcgaga aattaattct ccataggaga gcaatatcac
     6661 atcgcagaat tacagtgaga acgtgcataa atttagcggg aaaagacata aggaaaagcc
     6721 aatttgtcag acaaattgtt gaatgcacag cagattaatc cataagatta gcctggaaat
     6781 ccttgttgtc tttggtaccc atgcgggatg tcttcttttt caccagtcaa taggccgcat
     6841 tacctggcgt tgagtttttg aaatggtgta ataaccgcaa ctcaaagatg tggaaaatgc
     6901 acgtcattca tttcgtcatt aattatcact gtgctcatta attaacagaa cacgtataat
     6961 gagagccatc tcgcaaaaat gaaaaaacgt tttataaaat aatcacttca tcatgaattc
     7021 aaattcattg attaatatca acaagataca aaaagcacta tcattaaaat tcattgcagt
     7081 tacattgatt tcatcaatga aatgtaaaaa tatataaact tgatgattta agcattttct
     7141 tatacccgtt cagacgttat tcttatttca gatcatcgtc agaattgact ccacgatcac
     7201 atttcggacc ggcagaaagg aattattctg caaacagtaa ttatggtgtt ttgatttatc
     7261 ttgcacctct ccacttctgg atataaggat attaggtatg gcaaccgctg gaatgcttct
     7321 caaactcaac tctcaaatga accgcgagtt ttacgcatcc aatctctacc ttcacctgag
     7381 taactggtgt tctgaacaga gtctgaacgg caccgccact ttccttcgcg cccaggcaca
     7441 gagtaatgtg acccaaatga tgcgcatgtt taactttatg aagagtgtcg gcgctacccc
     7501 catcgttaaa gccattgatg ttcccggtga aaaactgaac tctctggaag aactgttcca
     7561 aaaaacgatg gaagaatacg agcaacgttc tagtacgttg gcacagttag ccgatgaagc
     7621 gaaagaactg aatgatgatt caaccgtcaa tttcctgcgc gatctggaaa aagaacagca
     7681 gcatgatggc ctgttgctgc aaaccattct tgatgaagtg cgcagtgcga aacttgcggg
     7741 tatgtgccca gtgcagaccg accaacatgt tctgaatgtc gtgtcgcatc agctgcattg
     7801 atcatacatc gacgctaatg cattgcaccg atgaagtttt tgagaaaccg ctgcctcatc
     7861 ttttttgaag cagcggtttt ttaatgggat tcgccttgtg gggtaaactt gagctcaatc
     7921 agcgcgatgg ctttttggat tgcccgcatg gtgaccgggt ctgcggcagc gggatgatga
     7981 gtaaagtcga tattcttcag ctgactggac attttttcac gaacttcaac gggcgcgatt
     8041 acatcgagaa catccagaat ttgtttgata accaactggc aagcaaccac atcagaaacc
     8101 agttcctgat cggcattcag cgtctgggac atcgtaaact cctgatagca ttttgaaagc
     8161 cgttatagta gcggcttcac atcttcaacg atagtcacat cccccctcat caggacacaa
     8221 aaaaacctgc cggagcaggt tttttgttat cggaacatat tgcctggcgg tacgtctttg
     8281 aacgtcttgc aatagttatt gaacatactt ttcaggattt tgcgcagttt catcgcggca
     8341 ctccgaccat ttgttataca ggtgttattg tctttgcgct tataatatga caaccatcac
     8401 aaaaatcaat ctttatgtga tacaaatcac ataaataccc ctttaatgtt ataaaaatga
     8461 taatcaaaga acagccccct atttctgaca cctacagatg gcaagaaata gcgcctgcca
     8521 ggcgtctttt ccggccattg tcgcagcgct gtaacgcgta aaatagtgct ttctcttact
     8581 cttctggctg gaccatgaga cttctgattc tgactctttc attaataacg ctggcggggt
     8641 gtacggttac tcgccaggcc catgtgagcg aagttgacgc ggcaacaggt attgtacggt
     8701 tggtttatga ccaggctttt ttgcagcatg cccataccga tcgttatgtc agtcgcggga
     8761 tctccgatcg cgcatgccaa caggaaggct atacccacgc gatccccttt ggtcagccag
     8821 taggcaactg tagccttttt gcgggttcct tatgtttgaa tacggaattc actttgtcat
     8881 atcagtgcca tcattccgcc ttccctgtct ttctctaaat attttaatca gcgaggggat
     8941 cttcgctgat taaagaaata gcggtaatgc gttttatt
//
LOCUS       DAZFAB010000149         8681 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZFAB010000149 DAZFAB010000000
VERSION     DAZFAB010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8681
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.149"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(237..1289)
                     /gene="fbaB"
                     /locus_tag="ACHJWA_004289"
     CDS             complement(237..1289)
                     /gene="fbaB"
                     /locus_tag="ACHJWA_004289"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFO4310765.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            1546..2823
                     /locus_tag="ACHJWA_004290"
     CDS             1546..2823
                     /locus_tag="ACHJWA_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFO4310766.1"
                     /translation="MKTTTKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFTGAALMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            2820..3824
                     /locus_tag="ACHJWA_004291"
     CDS             2820..3824
                     /locus_tag="ACHJWA_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFO4310767.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPNLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFINDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRTTTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGINAIDPALKAELDAVNQLDFNRYATALAKYRQQREAA
                     "
     gene            3821..4786
                     /locus_tag="ACHJWA_004292"
     CDS             3821..4786
                     /locus_tag="ACHJWA_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFO4310768.1"
                     /translation="MSGARLHTLLPELTTRQPVMVIGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPEALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFATPLIIRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(4760..5506)
                     /locus_tag="ACHJWA_004293"
     CDS             complement(4760..5506)
                     /locus_tag="ACHJWA_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO4310769.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(5558..6376)
                     /locus_tag="ACHJWA_004294"
     CDS             complement(5558..6376)
                     /locus_tag="ACHJWA_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFO4310770.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIHIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPVIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGSVDFNVFNGTEEELQAFVDGIKETP"
     gene            complement(6441..7241)
                     /gene="thiD"
                     /locus_tag="ACHJWA_004295"
     CDS             complement(6441..7241)
                     /gene="thiD"
                     /locus_tag="ACHJWA_004295"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFO4310771.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRALLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(7238..8026)
                     /gene="thiM"
                     /locus_tag="ACHJWA_004296"
     CDS             complement(7238..8026)
                     /gene="thiM"
                     /locus_tag="ACHJWA_004296"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFO4310772.1"
                     /translation="MQVDLLSSAQSAHTLHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGVANGGRGVDTTDAAVNAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDML
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(8061..8157)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8360..8599
                     /locus_tag="ACHJWA_004297"
     CDS             8360..8599
                     /locus_tag="ACHJWA_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor"
                     /protein_id="HFO4310773.1"
                     /translation="MTETQIYENIKQAISSAPRNSQTMEMHLQMIKYADHLKNVTAKE
                     FCEGVGLKASFATEFSKMRNLTERLKAAGLDTTKL"
BASE COUNT         2038 a         2253 c         2229 g         2161 t
ORIGIN      
        1 cgagataacg atcacaaaaa cgacaatatg aaaattaata gaggaatgaa aggcaaaaaa
       61 aacggcctcc cggagggaag ccgcaacaaa gtgcgcgtgg ttttatgcag gatgcggcgt
      121 aaacatatgt ggcagcgctg cgtaggcatg ataagacgct tcagcgtcgc atcaggcata
      181 ggttgccgga tgcggcgtaa acgccttatc cgtcctacgg ggtagtgccg ttcgtatcag
      241 gcgatagtaa ttttgctatc gagataaacg tcctgcacgg cgttaatcag tttcacgccg
      301 tcagccatcg atttcttgaa cgccttacgt ccaagaatca gccccattcc gccagcgcgt
      361 ttgttgataa ccgcagtacg cactgcatca ctgaggtcag tttcaccgcc tgcagcacca
      421 ccggagttta tcaacccggc ccggcccata tagcagttag ctaactgata acgcaccaga
      481 tcaatcgggt tttcgctggt taacttgctg tacacgcgat cgtcggtata accgtaatta
      541 attgctttat agccgccgtt attttccgcc attttttgtt tgacgatatc tgcacctatg
      601 gttgccgcca gatggttcgc ctgaccagtc aggtcggcgg agacatggta atcaacgcca
      661 tctttcttaa aggcggagtt acgcaaatag gcccacagca ctgtcaccat gcccagctcg
      721 tgcgcacgtt caaaagccgc agaaatttct tcaatctggc gacgtgactc ttccgagcca
      781 aaatagatag tcgcaccaac cgccaccgcg cccatgttga aggcctgctc cacgctggca
      841 tacagcgttt gatcgtaggt attcgggtaa cttagcgtct cgttgtgatt aagttttacg
      901 agaaatggaa tgcgatgcgc atagcgccgc gataccgacg ccaacacgcc gtaagttgat
      961 gccacacagt tacagcccgc ttcgatcgcc agttcaacaa tgtttttcgg gtcaaagtag
     1021 agcgggttag cagcaaatga agctccggca gagtgctcaa cgccctggtc aaccggcaga
     1081 atagaaagat atcctgtgcc agccagacgc ccggtgttgt acaacgtctg catattacgt
     1141 aacaccgctg gcgggcgatt attgtcaatc atcacgcggt ctacgtagtc atgtccgggg
     1201 agataaagct ggtcagaagg aatagtcata caacggtgct gtaaaaggtt gtcggcgtct
     1261 ttgccaagca actgcgcaat atctgtcatt actatgctcc cgtaaattcc gattggatat
     1321 cggctatgga ttgtcctggc ccgccttttg cgggcaatca taatcctggt cgttacgggc
     1381 gagtttttcc atcttttgga tgttttttca gcgttttctg ttggctcgat tcatcagaaa
     1441 aaatgttagc gcggtcaagt tttcaccgca aaggtattta aaaggtatta ttaagtggta
     1501 ttgtcatcgc gtaccttaca ttacctgtca tgaaggaatt aaaagatgaa aacaacaaca
     1561 aagctgtcgt tcatgatgtt tgttgaatgg tttatctggg gcgcgtggtt tgtgccattg
     1621 tggctgtggt taagtaaaag cggttttagt gccggagaaa ttggctggtc gtatgcctgc
     1681 acagccattg cggcgatcct gtcgccaatt ctggttggct ccatcactga ccgctttttc
     1741 tcggcgcaga aagtgctggc ggtattgatg ttcactggtg cggcgctgat gtatttcgct
     1801 gcgcaacaga ccacttttgc cgggttcttc ccgttactgc tggcctactc gctaacctat
     1861 atgccgacca ttgcgctgac taacagcatc gcttttgcca acgtgccgga tgtggagcgt
     1921 gatttcccgc gcattcgtgt gatgggcact atcggctgga ttgcctctgg tctggcatgt
     1981 ggtttcttgc cgcaaatgct ggggtatgcc gatatctcac cgactaacat cccgctgctg
     2041 attaccgccg gaagttctgc tctgctcggt gtgtttgcgt ttttcctgcc cgacacgccg
     2101 ccaaaaagca ccggcaaaat ggacattaaa gtcatgctcg gcctggatgc gctgatcctg
     2161 ctgcgcgata aaaacttcct cgtctttttc ttctgttcat tcctgtttgc gatgccactg
     2221 gcgttctatt acatctttgc caacggttat ctgaccgaag ttggcatgaa aaacgccacc
     2281 ggctggatga cgctcggcca gttctctgaa atattcttta tgctggcatt gccgtttttc
     2341 accaaacgct ttggtatcaa aaaggtatta ttgcttggtc tggtcaccgc tgcgatccgc
     2401 tatggcttct ttatttacgg tagtgcggat gaatatttca cctacgcgtt actgttcctc
     2461 ggcattttgc tgcacggcgt aagttacgat ttttactacg ttaccgctta catctatgtc
     2521 gataaaaaag cccccgtgca tatgcgtacc gctgcgcagg gactgatcac gctctgctgc
     2581 cagggcttcg gcagtttgct cggctatcgt cttggcggtg tgatgatgga aaagatgttc
     2641 gcttatcagg aaccggtaaa cggactgact ttcaactggt ccgggatgtg gacttttggc
     2701 gcggtgatga ttgccattat cgccgtgctg ttcatgatct ttttccgcga atccgacaac
     2761 gaaattacgg ctatcaaggt cgatgatcgc gatattgcgt tgacacaagg ggaagttaaa
     2821 tgaaaacaga acgtattctc ggtgctcttt atgggcaggc gttaggggat gcgatgggga
     2881 tgccctccga gctttggcca cgcagccgcg ttaaagcaca ctttggctgg attgaccgtt
     2941 ttctgcctgg accaaaggag aacaacgcgg cctgttattt taaccgcgcc gaattcaccg
     3001 acgatacctc gatggcgctg tgtctggcgg atgcgttact ggaacgtgaa ggcaagatcg
     3061 acccgaatct gattgggcgt aatattctcg actgggcgct gcgtttcgac gcctttaaca
     3121 aaaacgtact aggtccgacc tcgaagattg cgcttaacgc cattcgcgac ggtaaacccg
     3181 ttgctgaact ggaaaacaac ggcgtgacca acggcgcagc gatgcgcgtc tcgccattag
     3241 gttgtttgct tccggcgcgt gatgttgatt cctttattaa tgatgtggcg ctggcgtcga
     3301 gcccgaccca taaatccgat ctggcggttg cgggcgcggt agtcatcgca tgggcgattt
     3361 ctcgtgctat tgacggagaa agctggtcag cgattgttga ttcattgcct tcaattgcgc
     3421 gacatgcaca gcaaaaacgc accaccacgt tcagcgcctc actggcagcg cggctggaga
     3481 ttgcgctgaa aattgtgcgc aatgccgacg gcactgaatc cgccagcgaa cagctttacc
     3541 aggtcgttgg cgcaggtacc agcactattg agtccgttcc gtgcgccatt gcgctggttg
     3601 aactggcaca aaccgacccg aatcgctgcg ccgtcctgtg cgctaacctt ggcggcgata
     3661 cagacaccat cggtgctatg gcgacggcaa tctgcggcgc gttgcatggc attaacgcta
     3721 tcgatcctgc gttaaaggcg gagttggatg cggtaaatca gcttgatttc aaccgctatg
     3781 ccacagcgct ggcgaaatat cgtcaacaac gggaggcggc atgagcggcg ctcgattaca
     3841 cacgctgctg cctgaattaa ccacgcgtca gccggttatg gtgattggcg cggcagtcat
     3901 tgatgtgatc gccgacgctt atgccctccc ctggcgtggg tgcgatatcg aactgaaaca
     3961 gcagagcgtt aacgttggcg gctgcgcact gaatattgcc gtggcgttaa agcgcctcgg
     4021 catcgaagcg ggtaacgcct tgccgctcgg tcagggcgtg tgggcggaga ttattcgcaa
     4081 ccggatggca aaagagggct taatcagcct gatcgataac gccgaaggtg ataacggctg
     4141 gtgtctggcg ctggttgagc cggatggcga acgcactttt atgtcattca gcggtgtgga
     4201 aaatcagtgg aatcgccagt ggctggcgcg attaaccgtt gcgcctggca gcctgctcta
     4261 tttttccggt tatcaactgg cctcgccctg cggcgaatta ttagtggaat ggctggaaaa
     4321 gctgcaagac gtgacgccgt ttatcgattt tggcccacgt attggcgata tcccggaggc
     4381 attactggcg cggatcatgg cctgtcgacc tttagtgtcg ctcaatcgtc aggaggctga
     4441 gattgccgcc gaacgttttg ctttatccgc agagataaca acacttggca agcaatggca
     4501 ggagaaattt gccacgccgt tgatcattcg tctcgataaa gagggcgcat ggtatttcag
     4561 caacgacgct tctggctgca ttccggcatt tcccacgcaa gttgtagaca ccattggggc
     4621 gggcgacagt catgcaggtg gcgtacttgc cgggttggcc tctggtctgc cactggcgga
     4681 tgccgtatta ctgggcaatg cagtggcgtc gtgggttgtc gggcatcggg gcggtgattg
     4741 tgcgccaacg cgcgaggaac tactcctcgc acacaaaaac gtatagatcg ctgcgacagt
     4801 ggctaatgct gtactcaata ggccgttgct gttggtcaag cgccacttgc ttgatcacca
     4861 gcaccggtat tttgctatct aactgaatat gtgactgaaa ctcggcatct ggcatccggg
     4921 cgctaacgcg ggaacgtgta cgttgtgggt aaatgtgctg gctgcggaaa tagtcataaa
     4981 gagaaattcc gatggcatca acatcatgaa ttaaatgcgc cggaacccac gattcctcaa
     5041 tcgataccgc ttcttcatcg acatagcgaa tacgcttaag caaaaagaca tcacttcccg
     5101 cttcgaccgc cagctgctgc gcgacttctt cgggacattt cacgacacgc ttgttaaccc
     5161 atagcgtatc gggctttttc ccgcgcaata ccacctgctg agaaaaacct cgcgcttctt
     5221 tcaacgagta ttcgaagatg ttgttgatct gcgtgccgta accgcgcgaa cgcgtcacca
     5281 caccctcttc ttccagcgcc tgcatcgcct tgcgcaccgt aatgcgtgaa acgccggtta
     5341 actgacttaa atcacgctca ccgggcaaaa tattgccatg ctccagcacc ccgctgcgca
     5401 cggcattttt taccgtttcg gcaaacttaa tataaagcgg cgtgttatcc gccgctaaaa
     5461 tacgctcgtt cagttgggca atcagctggg tatgcgcttg ttccatttgt ctttttcctg
     5521 ccagggagtc tggtgggagt atatcactgc ttcttattta aggcgtttct ttaatcccat
     5581 caacgaatgc ctgcaactcc tcctctgtgc cattaaacac attaaaatcc accgaaccat
     5641 taatgccatc tacctgccca cggtcggaat gctgccagaa acgccaggcc atgccgtcat
     5701 tgtccgggcg acgttgatag tagtgagcca cccaccacgg atattcattg aaatagcccg
     5761 ccagattggt gtgataaaaa acggctcctg agtaaataac cggctttttt cccgtacttt
     5821 tttcgaccat ttttagccac tgacttaccc gcttgcgtaa ttcttttgcc gataattttc
     5881 cccgttcttc tacgtccagc acggcaggga aatcgccttg tgagaaatcc accgtttgca
     5941 gaaataatcg cgcctgaaca ggcgctgcta ccgacgggga gaaataatga tacgccccgc
     6001 gcagcaggcc attttcgcgg cttagttgcc agttacgcga aaaatagggg tccaccagct
     6061 tttcgccttc cgtcgccttg ataaaggcaa attgtaagcg gataccgtta tcgcgcattt
     6121 ttgccacccg ctgccagtcg atccgctcct gccagcgcga aacatctatt ccgtgaatgg
     6181 tgtaactggc aggaatatga atagcgaaag atttaaccgg gcgataaccg tagaagtgga
     6241 tgtagtctgt aatctgaact gctgtcgaat agaaaaaatt caccgtctga cgcggataaa
     6301 ttgcaacgat ggaaattagc ccaagagcgc ataatagagc ggtgagtttt tttcgactgg
     6361 taattcttag ctgcattggt ttcatccctg aatgtcagtg ccaaaggctg acaataacca
     6421 aagcagctat agtacggtgc tcaccaccag gcgtggaagt ggtgaaccgg accaataccg
     6481 tgaccaactt ccagcgtgtc ggcctgggct aacgccgatg aaagccagct ttttgcctcc
     6541 tgtacggtgt cagcccagtt tgtatggcgc gggcgtagtg cagccaacgc cgcagagagt
     6601 gtacaaccag tgccgtgggt gtttttggtc ataatgcgcg gtgcggtaaa ccgttgttca
     6661 ccctcgcggg taaacagcca gtccgggctt tgctcatcat ccagatggcc acctttcatt
     6721 agcactgcgc cacagcccat cgccaacagc gctcgccctt gttccagcat ttcctgttcg
     6781 gtgcgcgcgt gtggcgcgtc gagcaaggca gcagcttcgg gcaagtttgg cgttattaat
     6841 gaaacctgtg gcaataatcg actgcgtagc gtggcaactg ccgaaggtga aagcagtggg
     6901 tcgccacttt ttgccagcat aacggtgtcg agtaccacgt tttggatctg ataacgttgc
     6961 aaccgttctg ccaccgcttc aacaatatcg gtttccgcca acataccgat tttagtggta
     7021 tcgattcgca catcgctgaa caccgaatcg agctgggcgg cgacaaaatc aggctcaatg
     7081 cgatacaccg actgtacgcc acgggtattt tgcgccacca gtgcagtaat aactgagcaa
     7141 ccataagcgc caagtgccga gaaggtttta agatcggcct gaatccccgc accaccactc
     7201 ggatcagtac cggcaatcgt cagagcgtta attcgtttca tgcctgcacc tcctgcgtca
     7261 attgccagag cgcatcaagg aaatgtggaa caaaactgcc tggcccctcg cttctggcga
     7321 ctgcgcgttc tccggcttgt ttcatccagt gacaggcaga tgcgacattt tccagcatat
     7381 cgcctggtaa cgcacaacag gcagcgacaa ccgccgataa tgcacagcca gttcctacca
     7441 ctttggtcat taacggatcg ccaccgtgaa tgccaacggc gcgatggcca tcggtaacat
     7501 aatccacctc gccagtgacc acgacgattg cgccagtttc ccgtgccagt gtttgtgcag
     7561 cgggtatcgc gttaactgcg gcgtcagtgg tatccactcc ccgtccacca ttagcaacgc
     7621 cagctaatgc catgatttcc gaagcattac cacgtatcgc tgccggttta aaagataaaa
     7681 gttcatgaca aaaatggcgg cgataatcga gcgcaccaac cgctactgga tcaagcgtcc
     7741 agggtgtttg agagcttttt gcttgctcaa cggcagcacg catcgcctgt gcgcgtggct
     7801 gcgtcagtgt gccaacgtta atcaacaagg cactggcgat agccgcaaac tgactggcct
     7861 cttcggtttc gataaccatc gctggcgatg caccgagcgc cagcaaggta ttggcagtaa
     7921 aggtttgcac cacatcgttg gtcatgcagt gcacaagagg ggaatgttgg tgaaaaaggt
     7981 gtaacgtgtg cgcagattgc gcggaactca gcaggtcgac ttgcatagtt tgctcctgcc
     8041 ataacgtgaa gaagcaatga cctggtggtc cgtgacttcc ctacgctggc attatccaga
     8101 tcaggtgata cgggtatttc tcagccttca cgcagaaggg caccccgagt cgtttggttg
     8161 aatctctata ttttgtgcca gcttagggga agaggcttgc agatgcaagc tcgcctccat
     8221 tcattacgtc ttcactgtgt accccatatc agtaatgaaa aagaaacaat tcacacccat
     8281 cattcaggtg gtaaaaactg tagactatct taattagcta atttatatta tttatctaaa
     8341 tttgataggt gagataaaaa tgacagaaac ccagatctac gagaacatta agcaagccat
     8401 tagcagcgca ccacgaaata gccaaacgat ggaaatgcac ctccagatga tcaaatacgc
     8461 tgaccatctg aaaaacgtca cggcaaaaga gttttgcgaa ggtgtaggac ttaaagccag
     8521 ctttgctaca gagttcagca aaatgagaaa tcttaccgag cgactcaaag ccgccggact
     8581 tgataccacg aagttataac aaacgaaatc atcaatatat tactttcgtt ttccagggtt
     8641 ccctgtatat gtacctatga tcataaaaac cagttaccac c
//
LOCUS       DAZFAB010000150         8633 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZFAB010000150 DAZFAB010000000
VERSION     DAZFAB010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8633
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.150"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(275..538)
                     /gene="rpsT"
                     /locus_tag="ACHJWA_004298"
     CDS             complement(275..538)
                     /gene="rpsT"
                     /locus_tag="ACHJWA_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFO4310774.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            641..865
                     /locus_tag="ACHJWA_004299"
     CDS             641..865
                     /locus_tag="ACHJWA_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFO4310775.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGSQFLPVICAMTMT
                     MKSHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            873..1814
                     /gene="ribF"
                     /locus_tag="ACHJWA_004300"
     CDS             873..1814
                     /gene="ribF"
                     /locus_tag="ACHJWA_004300"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFO4310776.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1857..4673
                     /gene="ileS"
                     /locus_tag="ACHJWA_004301"
     CDS             1857..4673
                     /gene="ileS"
                     /locus_tag="ACHJWA_004301"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFO4310777.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPDEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFAHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYTEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4673..5167
                     /gene="lspA"
                     /locus_tag="ACHJWA_004302"
     CDS             4673..5167
                     /gene="lspA"
                     /locus_tag="ACHJWA_004302"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFO4310778.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVTLVVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            5255..5704
                     /gene="fkpB"
                     /locus_tag="ACHJWA_004303"
     CDS             5255..5704
                     /gene="fkpB"
                     /locus_tag="ACHJWA_004303"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO4310779.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5706..6656
                     /gene="ispH"
                     /locus_tag="ACHJWA_004304"
                     /gene_synonym="lytB"
     CDS             5706..6656
                     /gene="ispH"
                     /locus_tag="ACHJWA_004304"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFO4310780.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6722..7636
                     /gene="rihC"
                     /locus_tag="ACHJWA_004305"
     CDS             6722..7636
                     /gene="rihC"
                     /locus_tag="ACHJWA_004305"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFO4310781.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLSTLPQLNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            7803..8624
                     /gene="dapB"
                     /locus_tag="ACHJWA_004306"
     CDS             7803..8624
                     /gene="dapB"
                     /locus_tag="ACHJWA_004306"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFO4310782.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKENGLFDMRDVLDLNNL"
BASE COUNT         1969 a         2167 c         2447 g         2050 t
ORIGIN      
        1 aaatagagtg gtcttaagtt taatacctca atgtgtatca caatatccac attctttgta
       61 ggggagtctg gagattgagt agatattctt gttcagaatg tatcagccga tggttctacg
      121 attcttaagc cacgaagagt tcagatagtg caacggcatg tctcttttga ctatctggca
      181 accggcagtg tgttctctca cgcatcacaa aagcagcagg cataaaaaaa cccgcttgcg
      241 cgggcttttt cacaaagctt cagcaaattg gcaattaagc cagtttgttg atctgtgcag
      301 tcaggttagc cttatgacgt gcagctttgt ttttgtggat cagaccttta gcagcctgac
      361 ggtccacgat cggttgcatt tcgttaaatg ctttctgtgc agcagctttg tcgccagctt
      421 cgatagctgc gtatactttc ttgatgaaag tacgcatcat agagcgacgg cttgcgttgt
      481 gcttacgagc cttttcagac tgaatggcgc gcttcttagc tgatttgata ttagccaagg
      541 tccaactccc aaatgtgttc tatatggaca attcaaaggc cgaggaatat gcccttttag
      601 ccttcttttg tcaatggatt tgtgcaaata agcgccgtta atgtgccggc actcgttacg
      661 tagtgatggc gcaggattct accagcttgc ggggtgtgaa tacagctttt ccgcgataaa
      721 aattgcagca ggcagtcagt ttcttcccgt gatttgcgcc atgaccatga caatgaaaag
      781 ccacttcttt ctgatttcgg tactcaatcg ccggttaacc ttgaccgctg tacaaggtat
      841 actcggacga ttttcactgt tttgagccag acatgaagct gatacgcggc atacataatc
      901 tcagccaggc cccgcaagaa gggtgtgtgc tgactattgg taatttcgac ggcgtgcatc
      961 gcggtcatcg cgcgctgtta cagggcttgc aggaagaagg gcgcaagcgc aacttaccgg
     1021 tgatggtgat gctttttgaa cctcaaccac tggaactgtt tgctaccgat aaagccccgg
     1081 cacggctgac ccgactgcgg gaaaaactac gttaccttgc agagtgtggc gttgattacg
     1141 tgctgtgcgt acgtttcgac aggcgtttcg cggcgttaac cgcgcaaaat ttcatcagcg
     1201 atcttctggt gaagcatttg cgggtaaaat tcctcgccgt gggggatgat ttccgctttg
     1261 gcgctggtcg ggaaggcgat ttcttgttat tacagaaagc tggcatggaa tacggcttcg
     1321 atatcaccag tacgcaaact ttttgcgaag gtggcgtgcg tatcagcagc accgccgtgc
     1381 gtcaggccct tgcggatgac aatttggctc tggcagaaag tttactgggg cacccgtttg
     1441 ccatctccgg gcgtgtagtc cacggtgatg aattagggcg cactataggt ttcccgacgg
     1501 cgaatgtacc gctacgccgt caggtttccc cggtgaaagg ggtttatgcg gtagaagtgc
     1561 tgggcctcgg tgaaaagccg ttacccggcg tggcaaacat cggaacacgc ccaacggttg
     1621 ccggtattcg ccagcagctg gaagtgcatt tgttagatgt tgcaatggac ctttacggtc
     1681 gccatataca agtagtgctg cgtaaaaaaa tacgcaatga gcagcgattt gcttcgctgg
     1741 acgaactgaa agcgcagatt gcgcgtgatg aattaaccgc ccgcgaattt tttgggctaa
     1801 caaaaccggc ttaagcctgt tatgtaatca aaccgaaaca cggaaccgag aatctgatga
     1861 gtgactataa atcaaccctg aatttgccgg aaacagggtt cccgatgcgt ggcgatctcg
     1921 ccaagcgcga accgggaatg ctggcgcgtt ggactgatga tgatctgtac ggcatcattc
     1981 gtgcggctaa aaaaggcaaa aaaaccttca ttctgcatga tggccctcct tatgcgaatg
     2041 gcagcattca tattggtcac tcggttaaca agattctgaa agacattatc gtgaagtcca
     2101 aagggctttc cggttatgac tcgccgtatg tgcctggctg ggactgccac ggtctgccga
     2161 tcgagctgaa agtagagcaa gaatacggta agccggatga gaaattcacc gccgccgagt
     2221 tccgcgccaa gtgccgcgaa tacgcggcga cccaggttga cggtcaacgc aaagacttta
     2281 tccgtctggg cgtgctgggc gactggtcgc acccgtacct gaccatggac ttcaaaactg
     2341 aagccaatat catccgcgcg ctgggcaaaa tcatcggcaa tggtcacctg cacaaaggcg
     2401 cgaagccggt gcactggtgc gtagactgcc gttctgcact ggcagaagcg gaagttgagt
     2461 attacgacaa aacttctccg tccattgacg tcgctttcca ggcggtcgat caggatgcgc
     2521 tgaaagcgaa atttggcgta agcaacgtta acggcccaat ctcgctggtg atctggacca
     2581 ctacgccgtg gactctgcct gcgaaccgcg caatctctat tgcacctgat ttcgactatg
     2641 cgctggtgca gatcgacggt caggccgtga ttctggcgaa agatctggtt gaaagcgtaa
     2701 tgcagcgtat cggcgtgacc gattacacca ttctcggcac ggtaaaaggt gcggagcttg
     2761 agctgctgcg ctttgcccat ccgtttatgg gcttcgacgt cccggcaatc ctcggcgatc
     2821 acgttaccct ggatgccggt accggtgccg ttcacaccgc gcctggccac ggcccggacg
     2881 actatgtgat cggtcagaaa tacggcctgg aaaccgctaa cccggttggc ccggacggca
     2941 cttatctgcc gggcacttat ccgacgctgg atggcgtgaa cgtcttcaaa gcgaacgaca
     3001 tcgtcgttgc gctgctgcag gaaaaaggcg cgctgctgca cgttgagaaa atgcagcaca
     3061 gctatccgtg ctgctggcgt cacaaaacgc cgatcatctt ccgcgcaacg ccgcagtggt
     3121 tcgtcagtat ggatcagaaa ggtctgcgcg cgcagtctct gaaagagatc aaaggtgtgc
     3181 agtggatccc ggactggggc caggcgcgta tcgagtcgat ggtcgctaac cgtcctgact
     3241 ggtgtatctc ccgtcagcgt acctggggcg taccgatgtc tctgttcgtg cacaaagaca
     3301 cggaagagct gcatccgcgt accctcgaac tgatggaaga agtggctaaa cgcgttgaag
     3361 ttgatggcat ccaggcgtgg tgggatcttg atgcgaaaga gatcctcggc gatgaagctg
     3421 atcagtatgt gaaagtgccg gatacgctgg atgtatggtt tgactccggc tctactcact
     3481 cttctgttgt tgacgtgcgc ccggaatttg ccggtcacgc tgcggacatg tatctggaag
     3541 gttctgacca acaccgtggc tggttcatgt cttctctgat gatctccacc gcgatgaagg
     3601 gcaaagcgcc gtatcgtcag gtgctgaccc acggctttac cgtagatggt cagggccgca
     3661 agatgtctaa atccatcggc aacaccgtgt cgccgcagga tgtgatgaac aaactgggcg
     3721 cggatattct gcgtctgtgg gtggcatcaa ccgactacac cggtgaaatg gccgtttctg
     3781 acgagatcct gaaacgtgct gccgacagct atcgtcgtat ccgtaacacc gcgcgcttcc
     3841 tgctggcaaa cctgaacggt tttgatccgg caaaagatat ggtgaaaccg gaagagatgg
     3901 tagtactgga tcgctgggcc gtaggttgtg cgaaagcggc acaggaagac atcctcaagg
     3961 catacgaagc atacgatttc cacgaagtgg tacagcgtct gatgcgcttc tgctccgttg
     4021 agatgggttc cttctacctc gacatcatca aagaccgtca gtacaccgcc aaagcggaca
     4081 gcgtggcgcg tcgtagctgc cagactgcgc tgtatcacat cgcagaagcg ctggttcgct
     4141 ggatggcacc aatcctctcc ttcaccgctg atgaagtgtg gggctacctg ccgggcgaac
     4201 gtgaaaaata cgtcttcacc ggcgagtggt acgaaggtct gtttggtctg gcagacagtg
     4261 aagcaatgaa cgatgcgttc tgggacgagc tgttgaaagt gcgtggcgaa gtgaacaaag
     4321 tcattgagca ggcgcgtgcc gacaagaaag tgggtggctc gctggaagcg gcagtaacct
     4381 tgtatacaga accggaactg gcggcgaaac tgaccgcatt gggcgatgaa ttacgatttg
     4441 tcctgttgac ctccggcgct accgttgcag actataacga cgcacctgct gatgctcagc
     4501 agagcgaagt gctcaaaggg ctgaaagtcg cgttgagtaa agccgaaggt gagaagtgcc
     4561 cacgctgctg gcactacacc caggatgtcg gcaaggtggc ggaacacgca gaaatctgcg
     4621 gccgctgtgt cagcaacgtc gccggtgacg gtgaaaaacg taagtttgcc tgatgagtca
     4681 atcgatctgt tcaacagggc tacgctggct gtggctggtg gtagtcgtgc tgattatcga
     4741 tctgggcagc aaatacctga tcctccagaa ctttgctctg ggggatacgg tcccgctgtt
     4801 cccgtcgctt aatctgcatt atgcgcgtaa ctatggcgcg gcgtttagtt tccttgccga
     4861 tagcggcggc tggcagcgtt ggttctttgc cggtattgcg attggtatta gcgttaccct
     4921 ggttgtgatg atgtatcgct cgaaggccac gcagaagcta aacaatatcg cctacgcgct
     4981 gattattggc ggcgcgctgg gcaacctgtt cgaccgcctg tggcacggct tcgttgtcga
     5041 tatgatcgac ttctacgtcg gcgactggca cttcgccacc ttcaacctcg ccgatactgc
     5101 catctgtgtt ggtgcggcac tgattgtgct ggaaggtttt ttgccttcta aagcgaaaaa
     5161 acaataataa accctgccgg ataaggccta cagattgctg cgaaatcgta ggccgcatcc
     5221 ggcaaaaatc ctcaaaatat aagagcaaac ctgcatgtct gaatctgtac agagcaatag
     5281 cgccgtcctg gtgcacttca cgctaaaact cgacgatggc accaccgccg agtctacccg
     5341 caacaacggt aaaccggcgc tgttccgcct gggcgatgct tctctttccg aagggctgga
     5401 gcaacacctg ctggggctga aagtgggcga taaaaccact ttctcgctgg agccagatgc
     5461 ggcgtttggc gtgccgtcac cggacctgat tcagtacttc tcccgccgtg agtttatgga
     5521 tgcaggcgag ccagaaattg gcgcaataat gctttttacc gcaatggatg gcagtgagat
     5581 gcctggcgtg atccgcgaaa ttaacggcga ctccattacc gtcgatttca accatccgct
     5641 ggccgggcag accgttcatt ttgatattga agtgctggaa atcgatccgg cactggaggc
     5701 gtaacatgca gatcctgttg gccaacccac gtggtttttg tgccggggta gaccgcgcta
     5761 tcagcattgt tgaaaacgcg cttgccattt acggcgcacc gatatatgtc cgtcacgaag
     5821 tggtgcataa ccgctacgtg gtcgatagcc tgcgcgagcg tggagctatc tttattgagc
     5881 agatcagcga agtgccggac ggcgcgatcc tgatcttctc cgcacatggt gtttctcagg
     5941 cggtacgtaa cgaagcgaaa agccgtgatt tgacggtatt cgacgccacc tgtccgctgg
     6001 tgaccaaagt gcatatggaa gtcgcccgcg ccagccgtcg tggcgaagag tctattctca
     6061 tcggtcacgc cgggcacccg gaagtggaag ggacgatggg gcagtacagc aaccctgaag
     6121 ggggaatgta tctggtcgaa tcgcctgacg atgtgtggaa actgacggtc aaaaacgaag
     6181 agaagctctc ctttatgacc caaaccacgc tgtcggtaga tgacacgtct gatgtgatcg
     6241 acgcgctgcg taaacgcttc ccgaaaattg tcggtccgcg caaagatgac atctgctacg
     6301 ccacgactaa ccgtcaggaa gcggtacgcg ccctggcaga acaggcggaa gttgtgttgg
     6361 tggtcggttc gaaaaactcc tccaactcca accgtctggc ggagctggcc cagcgtatgg
     6421 gcaaacgcgc gtttttgatt gacgatgcga aagatatcca ggaagagtgg gtgaaagagg
     6481 ttaaatgcgt cggcgtgact gcgggcgcat cggctccgga tattctggtg cagaatgtgg
     6541 tggcacgttt gcagcagctg ggtggtggtg aagccattcc gctggaaggc cgtgaagaaa
     6601 atattgtttt cgaagtgccg aaagagctgc gtgtcgatat tcgtgaagtc gattaagtca
     6661 ttagcagcct aagttatgcg aaaatgccgg tcttgttatc ggcatttttt atggagaaaa
     6721 catgcgctta cctatcttcc tcgatactga ccccggcatt gacgatgccg tcgccattgc
     6781 cgccgcgatt tttgcccccg aactcgacct gcaactgatg accaccgtcg cgggtaatgt
     6841 ctcggttgag aaaactaccc gtaacgcctt gcaactgctg catttctgga atgcggagat
     6901 tccgctcgcc cagggagccg ctgtgccgct ggtacgcgca ccgcgtgatg cagcatctgt
     6961 gcacggcgaa tcgggaatgg ctggctacga ctttgttgag cacaaccgaa agccactcgg
     7021 gataccggcg tttctggcga ttcgggatgc cctgatgcgt gcaccagagc ccgttaccct
     7081 ggtggccatc ggcccgttaa ccaatattgc gctgttactt tcacaatgcc cggaatgtaa
     7141 accgtatatt cgccgtctgg tgatcatggg tggttctgcc ggacgcggca actgtacgcc
     7201 aaacgccgag tttaatattg ctgccgatcc agaagctgct gcctgtgtct tccgcagtgg
     7261 tattgaaatc gtcatgtgcg gtttggatgt caccaatcag gcaatattaa ctcctgacta
     7321 tctctctaca ctgccgcagt taaaccgtac cgggaaaatg cttcacgccc tgtttagcca
     7381 ctaccgtagc ggcagtatgc aaagcggctt gcgaatgcac gatctctgcg ccatcgcctg
     7441 gctggtgcgc ccggacctgt tcactctcaa accctgtttt gtggcagtgg aaactcaagg
     7501 cgaatttacc tcaggcacga cggtggttga tatcgacggt tgcctgggca aaccagccaa
     7561 tgtacaggtg gcattggatc tgaatgtaaa aggcttccaa cagtgggtgg ctgaggtgct
     7621 ggctctggtg ccgtaacctg tcacatgtta tcggcatgca gtcattcatc gactcatgcc
     7681 tttcactgat atccctccct gtttatcatt aatttctaat tatcagcgtt tttggctggc
     7741 ggcgtagcga tgcgctggtt actctgaaaa cggtctatgc aaattaacaa aagagaatag
     7801 ctatgcatga tgcaaacatc cgcgttgcca tcgcgggagc cggggggcgt atgggccgcc
     7861 agttgattca ggcggcactg gcattagaag gcgtgcagct gggcgcggcg ctggagcgcg
     7921 aaggatcttc tttactgggc agcgacgccg gtgagctggc tggtgcaggg aaaacaggcg
     7981 ttaccgtgca aagcagtctt gatgcgataa aagatgattt tgatgtgttt atcgatttta
     8041 cccgtccgga aggtacgctg aaccatctcg ctttttgtcg ccagcatggc aaagggatgg
     8101 tgatcggcac tacggggttt gacgaagccg gaaaacaggc aattcgtgac gccgccgccg
     8161 atattgcgat tgtctttgcc gccaatttta gcgttggcgt taacgtcatg cttaagctgc
     8221 tggagaaagc agccaaagtg atgggtgact acaccgatat tgaaattatt gaagcacatc
     8281 atagacataa agttgatgcg ccgtcaggca ccgcactggc aatgggagag gcgatcgccc
     8341 acgcccttga taaagatctg aaagattgcg cggtctacag tcgtgaaggc cataccggtg
     8401 aacgtgtgcc tggtaccatt ggttttgcca ccgtgcgtgc aggtgacatc gttggcgaac
     8461 ataccgcgat gtttgccgat attggcgagc gtctggagat cacccataag gcgtccagcc
     8521 gcatgacatt tgctaacggt gcggtaagat cggctttgtg gttgagtggt aaggaaaatg
     8581 gtctttttga tatgcgagat gtgcttgatc tcaataattt gtaaccacaa aat
//
LOCUS       DAZFAB010000151         8621 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZFAB010000151 DAZFAB010000000
VERSION     DAZFAB010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.151"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..1891
                     /gene="espL4"
                     /locus_tag="ACHJWA_004307"
     CDS             <1..1891
                     /gene="espL4"
                     /locus_tag="ACHJWA_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
                     /protein_id="HFO4310783.1"
                     /translation="APIFEEYLMEILPQRSDALDKKELNLNSDRKEKEFPRVKLNGQC
                     YFPGRPQNRIVCRHIAAQYINDIYQNVDYKPHQDDYSSAEKFLTHFNKKCKNQTLALI
                     SSRPEGRCVAACGDFGLVMKAYFDKMESNDLSVMAAILLVDNHALTVRLRIKNTTEGC
                     IHYVVSVYDPNVTNDKIRIMSESKEDIKHYSLMDFMNVDYSLLKWSNDHVINQSVAII
                     PALPKEQLLMLKGTVDEITPPLSPATMNLLMAIGQNHQLKQLMIQLQKMPELHRTEML
                     TAYNSINLPGLYLAINYGNADIVETIFNSLSEPGYEGLLSKKNLMHILEAKDKNGFSG
                     LFLAISRKDKNVVTSILNALPKLAATHHLDNEQVYKFLSAKNSTSSHVLYHVMANGDA
                     DMLKIVLDALSLLIRTCHLTKEQVLDLLKAKDFYGCPGLYLAMQNGHSDIVKVILEAL
                     PSLAQEINISASDIVDLLTAKSLARDTGLFMAMQRGHMNVINTIFNALPTLFNTFKFD
                     KKNMKPLLLANNSNEYPGLFSAIQHKQQNVVEMVYLALSDHARLFGFTAEDIMDFWQH
                     KAPQKYSAFELACELGHRVIAELIFNTLNKMAESFGFTDNPRYIAEKNYMEALLKKAS
                     PHTVR"
     gene            complement(1860..3596)
                     /gene="aceK"
                     /locus_tag="ACHJWA_004308"
     CDS             complement(1860..3596)
                     /gene="aceK"
                     /locus_tag="ACHJWA_004308"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HFO4310784.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAEFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLHWQNKSRDIHYIIRHLTETLGPENLSKSHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(3780..5084)
                     /gene="aceA"
                     /locus_tag="ACHJWA_004309"
     CDS             complement(3780..5084)
                     /gene="aceA"
                     /locus_tag="ACHJWA_004309"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFO4310785.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAEAIHAKYPGKLLAYNCSPSFNWQKNLDDNTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(5114..6715)
                     /gene="aceB"
                     /locus_tag="ACHJWA_004310"
     CDS             complement(5114..6715)
                     /gene="aceB"
                     /locus_tag="ACHJWA_004310"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HFO4310786.1"
                     /translation="MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLEDRRVEITGSVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEEHNNQVLNKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(6984..7913)
                     /gene="metA"
                     /locus_tag="ACHJWA_004311"
     CDS             complement(6984..7913)
                     /gene="metA"
                     /locus_tag="ACHJWA_004311"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFO4310787.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPEVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            8070..8513
                     /locus_tag="ACHJWA_004312"
     CDS             8070..8513
                     /locus_tag="ACHJWA_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO4310788.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVKHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLDLAYVGE"
BASE COUNT         2214 a         2105 c         2112 g         2190 t
ORIGIN      
        1 tgctccaata tttgaagaat acctaatgga aattttacct caacgctcag acgctcttga
       61 taaaaaagaa ttaaatctaa actcagatag aaaagaaaaa gaattcccaa gagttaagct
      121 taatggtcaa tgttattttc cggggcgccc ccaaaaccgt atagtatgcc gacacattgc
      181 tgcacaatat attaatgata tttatcagaa tgttgactac aaaccccatc aagatgatta
      241 ctcttcagct gaaaaatttc tcactcactt caacaaaaaa tgcaaaaacc agactttggc
      301 gttgatttcc agccgtcctg aggggcgttg cgttgctgcc tgcggtgatt tcgggctagt
      361 tatgaaggca tattttgaca agatggaatc aaatgacctc agtgttatgg ctgccatatt
      421 attggtagat aaccatgctt tgacggtccg gctaagaata aagaacacaa ctgaaggatg
      481 tatccattac gtggtttcgg tttatgatcc taatgtaact aacgataaaa taagaatcat
      541 gagcgaaagt aaagaggata ttaaacacta ttcactgatg gattttatga atgtagatta
      601 tagcctcctg aaatggtcaa atgatcatgt tattaaccaa tctgttgcaa taattccagc
      661 acttccaaaa gaacagctat tgatgctaaa aggaactgtg gatgaaataa cccctccatt
      721 atcacctgca acgatgaatt tgctaatggc aattggtcag aatcaccaac ttaagcaact
      781 gatgattcaa ctccagaaaa tgccagaact acatagaaca gaaatgttga ctgcctataa
      841 tagtattaac ctccccggtt tatatttggc tataaattat ggtaatgcgg atatcgttga
      901 gactattttt aattcattgt cagagccagg atatgaagga ttactctcga aaaaaaatct
      961 catgcatatt ctggaggcaa aagataaaaa tggtttttct ggattatttt tagcgatatc
     1021 acgtaaggat aaaaatgttg taacctcgat tctgaacgcc ttacctaaac tggccgcaac
     1081 acatcattta gataacgaac aagtgtataa attcctgagt gccaaaaata gtacgtccag
     1141 ccatgtttta taccatgtta tggcgaatgg tgatgccgac atgctgaaaa ttgttttgga
     1201 cgcgttatct ttgttaattc gcacatgtca tttgactaaa gaacaggtac tcgatctcct
     1261 gaaggcaaag gatttttatg gttgcccagg actatacctg gcgatgcaaa atggacatag
     1321 cgatatcgtg aaagttattc ttgaagcatt gcccagcctg gcccaggaaa ttaacatttc
     1381 agcttccgat attgtagatc ttctgaccgc taaaagtctt gcgcgcgaca cgggtttgtt
     1441 tatggccatg cagcgcggac atatgaacgt tattaatact atttttaacg cattacccac
     1501 tctgtttaat acgtttaaat tcgataaaaa aaatatgaag cccctcctcc tggcaaataa
     1561 ttctaatgaa tacccaggtt tgttttcagc gatacagcat aaacagcaaa acgttgtaga
     1621 gatggtttat cttgctttat ctgaccatgc acgcctgttt ggatttaccg ctgaagatat
     1681 tatggatttt tggcaacaca aagcgccaca aaaatactct gcctttgagt tggcttgtga
     1741 attgggtcac cgggttattg ctgaattaat ctttaataca ttaaataaga tggctgaaag
     1801 ctttggcttt acggataacc ctcgatacat tgcggagaaa aattatatgg aagctttact
     1861 caaaaaagca tctccccata ccgtacgcta aatctttgcc tgcgccgata cgcataaaca
     1921 tcttccacat gcccttcacg tatgcggttt tgtagtgcgc gccagtaatc agcgcggaac
     1981 aggtcggcgt gcatctcttc aaacagcgga ccaatacgcg ggtcggcgca tagccaatgg
     2041 cgaaactctt ccgggaaaac atcgcccggc gagacgctgt accacggttc gctggcaagt
     2101 tcgtcttccg ggtagcgcgg cggcgggata tcgcggaaat tcacttccgt catgtagcaa
     2161 atttcatcgt aatcataaaa aaccacacgc ccgtgacggg tgacaccgaa gtttttaaac
     2221 agcatgtcgc ccgggaaaat gttagcagcg gcaagctggc gaatggcatt gccgtactct
     2281 tcaatggcgt cgcgcagctg ctgaccttcc acttgttcca gccagatatt gagcggcacc
     2341 atccgccgct caatataaag atggcgaatc acaatttgtt cgccgagatc ggtgattttt
     2401 tccgctgctt cctggagcag taattccatt aatgccgggg aaatatgccg cttctccagc
     2461 acaaagtttt caaactcctg ggtatccgcc attcggccca cgcgatcgtg ctctttcacc
     2521 agttgatagc aggcacgaac gtgagcggca gacatctctt tctgcggcgc gaacttgtct
     2581 ttgatgactt taaatacacg gtcaaagccc ggcagcgtga acaccaacat caccatgcca
     2641 cgaatacccg gcgcttcaat gaactgctca ttacagccct gtagataaac gagatattcg
     2701 cggtagcttt cggttttggc gtgcttctgg cagccgatag ccatatacaa ttcagcggtg
     2761 gttttacctg gcagaatttc ccgtaaccac tcgaccagcg ctgcgggcag cggcgcataa
     2821 accataaaat aagaacgcgc aaagccaaaa acaatgctcg cttcggcggt cgtcgtcaga
     2881 caggtatcaa taaacagttc gccgtcatcc gtctggtgga tcggcaataa aaatggcaat
     2941 gtgccggaag gcgttattag tttgcccacc agccaggcgg ctttattacg gtaaaacagt
     3001 tcgttcgcca cctgtaaatg acttttggag aggttttctg gccccaacgt ttccgtcaga
     3061 tggcgaataa tgtaatggat gtcacggctt ttattctgcc agtgcagacg caacggcagg
     3121 tcgctgataa cgcgcatcag cagagattcc cagccgtgat cggggtgaaa gtctttcgcc
     3181 agcgggcggg gaatggtacg aaagcggcgc tctggctggg agctaaagat aaaaagccgc
     3241 tcgggagtaa gcgagcggtg gtcaaataac cgacagtaca cggagttaaa aaagctctcc
     3301 gcaatctcga agcgcgggta atccggcaac agccgggtgt aatgctcttt gacgcgcagt
     3361 aaaaattccg cgtccgtgct ttggccgttg gtaatgcagc gcagttgctc cacgaccaga
     3421 ccaacgtgat gatcgtaaag atggatacgg tttttcatcg cctgctggac agcatgccag
     3481 tcggcttgtt cgaaacgctg ctgcgccccg gaagtcactt cgaggaatcg accatactga
     3541 gcgtcgaagc cttgcaaaat ggtttgagca atcagtaatt ccaggccacg cggcatttag
     3601 cgccctcatc aggagcagag aattgccgga tgcggcgtaa acgccttatc cagcctacgt
     3661 tcggcaacgg ttgtaggcat gataagacgc gcaagcgtcg catcaggcat cgtgcaccga
     3721 ttgccggatg cggcgtatat gccttatccg gcctacagtc agcaacgttt gtaggttgct
     3781 tagaactgcg attcttcggt ggagccagtc agcgcggtaa ccgacgacgt gccgccctga
     3841 ataatggtcg tcactttatc gaagtaacct gtacccactt cctgctggtg agatacgaag
     3901 gtgtagccat ctttcgcggc ggcgaattcc ggctgctgca ctttctcaac atagtgcttc
     3961 ataccctcgc cctgggcata ggcgtttgcc agatcaaaca tgttgaacca catgctgtgg
     4021 atacctgcca gggtgatgaa ctggaacttg tagcccatat ccgacagctg ctgctggaag
     4081 ctggcaatag tgttgtcgtc gaggtttttc tgccagttga acgacggcga gcagttataa
     4141 gccagcagtt tgcccggata tttcgcgtgg atagcttccg caaagcgacg cgccagttcc
     4201 agatccggcg tggaggtttc acaccatacc aggtcagcat atggcgcata cgccaggcca
     4261 cggctgatcg cttgctcaat gcccgcatga gtacggaaga agccttcact ggtacgctcg
     4321 ccggtaataa attcgctgtc atacgggtcg caatcggagg tgatcagatc cgccgcatca
     4381 gcatcggtac gtgcaaccag cagggttgga acgcccgtca cgtcagctgc cagacgcgcc
     4441 gcgaccagtt tctgaatagc ttcctgagtt ggcaccaaaa ctttgccgcc catgtgaccg
     4501 catttcttca ctgacgccag ctgatcttcg aagtgaactg ccgctgcacc ggcttcaatc
     4561 atcgctttca tcagttcaaa ggcattcagg acaccgccaa aaccggcttc cgcgtcggca
     4621 acgatcggca ggaagtaatc gacatagcgc ggatcgcccg gctcaatgcc cgcggaccat
     4681 tggatctgat ccgcacggcg gaaagtgttg ttgatccgct ccaccacagc tggcaccgag
     4741 tttgccggat agagcgactg atccggatac atgctggccg ccaggttagc gtccgccgct
     4801 acctgccatc ccgacagata gaccgcttca atacccgctt tcgcctgttg cagcgcctga
     4861 ccgccagtca gtgcaccgag gctgttgatg tagccttttt tcgactcgcc atgcagcaga
     4921 cgccacattt tcgccgcgcc cagttgcgcc agcgtgcatt caggattgac tgaaccgcgt
     4981 aatttcacca catcttccgc gctgtatggg cgagtaattc cttcccaacg cggttgagtc
     5041 cactctttct gtaattcttc aatttgttgt gtacgggttt tcatgtgcag atgctccata
     5101 gttatgtggt ggtttacgct aacaggcggt agcctggcag ggtcaggaaa tcaattaact
     5161 catcggaagt ggtgatctgt tccatcaagc gtgcggcatc gtcaaaacgc ccctgggaga
     5221 aacgttcttc gcccagttcg ctggcaatga ctttcatctc ttcgcccagc atctggcgga
     5281 acaaggcttt agttaccggt ttgccattgc tcaacgtttt ttgatgatgg atccactgcc
     5341 agattgaggt acgggaaatt tcagccgtcg ccgcatcttc catcaggcca taaatcggca
     5401 cgcagccgtt gccggagatc catgcttcga tgtactgcac agccacgcga atgttggcgc
     5461 gcataccttc ttcggtgcgt tcaccatcac aaggtgccag cagctgatcg gcagtaatcg
     5521 gcgcgtcttg ttcgcgcatc acttcaagct gatttttacg ggagccgaga atgtcgttga
     5581 ataccgccat tgccgtgtcc gcaaggcctg ggtgagcgat ccatgtgcca tcgtgaccgt
     5641 tattggcttc cagcgattta tccgctttta ctttgttgag cacctggtta ttgtgctctt
     5701 catctttgct cggaataaac gccgccatgc cgcccatcgc aaaagcaccg cgtttatggc
     5761 aggttttaat caacaggcgt gagtaagcat tcaggaatgg tttatccatc gttactgcct
     5821 gtctgtctgg caggacgcga tcgggatagt ttttcaacgt tttgatatag ctgaagatgt
     5881 aatcccaacg accgcagttc agaccaacaa tatggtcacg cagcgcgtga aggatttcat
     5941 ccatctggaa cacggcgggc agcgtttcaa tcagcaacgt cgccttgatg gtgccgcgcg
     6001 gcagattaaa gcgatcttct gcatagctga agacttcgct ccaccaggcc gcttcctgcc
     6061 aggactgggt tttcggcaga tagaaatagg gaccactgcc ctttgccaac agtgcctgat
     6121 agttgtggaa gaaatagagc gcaaaatcaa acaggctgcc ggggattgcc tcaccacgcc
     6181 aggtgacatg tttttccggc aagtgcagac cgcgtacccg acaaatcaaa accgctggat
     6241 tgggcttgag ctggtaaatt ttgcctgctt cattggtgta actgatggtg ccgttaaccg
     6301 catcacgcag gttaatttgc ccgtcgatca ctttgttcca gtctggtgcc agtgaatctt
     6361 cgaaatcggc cataaagact ttcacattgg cgttgagcgc gttgatcacc atcttgcgct
     6421 ctacggatcc ggtaatctct acgcggcgat cttctaagtc cgcaggaatc ccgcgaattt
     6481 tccagtcagc atcacgaatg gaagctgttt ccgaaataaa atcaggcaac gttccgttat
     6541 caatatcttg ctgctgctga atgcgcgctg ccagaagttt attgcgttgt ggcgtaaaat
     6601 gcgtcaccag ctcagtcaga aattctaccg cttcggcagt aagaatttgc ttctcctgct
     6661 cgccatacgg ccttatgaaa gccagttcat cggttgttgt tgcctgttca gtcatcgtgc
     6721 agctcctcgt catggatccg aaaacttccc cactgtgaac gaaggatcgt tgtgcgcttt
     6781 tcgttcagca caactaagac tactcattta aaaatcaaaa tcaaaaacaa tttccatttt
     6841 aattaaaaat acttgataac ttattgataa caatatgaat aaaatttatt cacctactga
     6901 ggtgtgtttc ggaaataaaa aaggcacccg aaggtgcctg aggtaaggtg ctgaatcgct
     6961 taacgatcga ctatcacaga agattaatcc agcgttggat tcatgtgccg tagatcgtat
     7021 ggcgtgatct ggtagacgta atagttgagc cagttggtaa acagcaaatt accgtgacta
     7081 cgccagctcg ctcgcggttt gttttgcgga tcattgtgcg ggaaatagtt atacggtact
     7141 tccgggtcta gtccggcttc cacatcgcgg aaatattcct gcgccagcgt ttgcgcatca
     7201 tattcgggat ggcccgtcac aaaggcaatg cgcttatctt tactggcaaa cagatatgca
     7261 tccccttctt ccgtctctgc cagaatttcc agatcggtgt aatcacgaat caacgctgcc
     7321 ggaaagtcag catagcgcga atgcggtgcc aagaatgaat catcaaagcc acgcgtcaga
     7381 agcgcatgag gatggagaat atgatgctcg taaacgccag agagtttgtc ggtgcgagtt
     7441 tgcttaggaa tgccgtagag gatattgagc gctgcctgta ccgcccagca gacaaacagc
     7501 gtcgaggtga cgtgatcttt cgaccactcc agcacctgtt tgatctgcgg ccagtaagcg
     7561 acatcattaa actccaccag gcccagcggc gcaccggtta caatcagacc gtcaaagttc
     7621 tgttcctgaa tatcttcaaa gttacagtag aagttgttca gatgctctgc gggcgtgtta
     7681 cgcgattcac gggaatcgat gcgcaacagc tgaatatcga cctgcaaagg tgagtttgaa
     7741 agcaggcgca gaaactgatt ttcagtttca atcttcttcg gcatcaggtt aaggatcaga
     7801 accttaagtg gacgaatttc ctgaccagac gcacgagaag ttgtcatcac aaagacgttt
     7861 tcttcacgca agaaattgac ggcgggtagc tcgtccggca cacgaatcgg cataacctga
     7921 ttacctcact acatacgctt atacgtttag acatccagat agctgaagat aaccagaaaa
     7981 tttgccaatg tcgagcctgc atgttgaagg tgagaaagtt tcaaggaaaa gactacgtta
     8041 gaatataaga atgactaaaa ggagagaaaa tggttattag tattcgccgc tcacggcatg
     8101 aggaagggga ggaactcgtt gcgatttggt gtcgatctgt cgatgccacc cacgattttt
     8161 tatcagcaga gtatcgggcc gagctggaag agctggttcg ttcctttctg ccggaagcac
     8221 cgttgtgggt cgcggttaat gaacgggatc agccggttgg gtttatgttg ttaagtgggc
     8281 agcatatgga tgcgctgttt atcgatcctg atgtgcgcgg ctgcggcgtg ggccggatgc
     8341 tggtgaagca tgcgctctca atggccccgg aactgacaac caatgttaat gagcaaaatg
     8401 agcaggcggt tgggttctat aagaaggtgg gttttaaggt tacgggacgc tctgaggtgg
     8461 acgatttggg gaaaccgtat ccgttgctgg atctggcgta tgtgggggaa taagctgccc
     8521 cctatcgtgc ctgcgtaagc tggataaaac gcgccagcgt cgcatccggc atttttttgc
     8581 accaacgcaa aaaggccatc cgtcaggatg gcctttcgct t
//
LOCUS       DAZFAB010000152         8427 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZFAB010000152 DAZFAB010000000
VERSION     DAZFAB010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8427
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.152"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(206..282)
                     /locus_tag="ACHJWA_004313"
     tRNA            complement(206..282)
                     /locus_tag="ACHJWA_004313"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Asp,seq:gtc)
     gene            complement(415..1146)
                     /gene="dnaQ"
                     /locus_tag="ACHJWA_004314"
     CDS             complement(415..1146)
                     /gene="dnaQ"
                     /locus_tag="ACHJWA_004314"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFO4310789.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRLVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            1211..1678
                     /gene="rnhA"
                     /locus_tag="ACHJWA_004315"
     CDS             1211..1678
                     /gene="rnhA"
                     /locus_tag="ACHJWA_004315"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFO4310790.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1675..2397)
                     /locus_tag="ACHJWA_004316"
     CDS             complement(1675..2397)
                     /locus_tag="ACHJWA_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO4310791.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFY
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2431..3186
                     /gene="gloB"
                     /locus_tag="ACHJWA_004317"
     CDS             2431..3186
                     /gene="gloB"
                     /locus_tag="ACHJWA_004317"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFO4310792.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            3258..4616
                     /gene="mltD"
                     /locus_tag="ACHJWA_004318"
     CDS             3258..4616
                     /gene="mltD"
                     /locus_tag="ACHJWA_004318"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFO4310793.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNSMP
                     DS"
     gene            complement(4664..5434)
                     /locus_tag="ACHJWA_004319"
     CDS             complement(4664..5434)
                     /locus_tag="ACHJWA_004319"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO4310794.1"
                     /translation="MTTQSHHDHVEKQFSSQASEYLTSTVHASGRDLQLLAVCLADYP
                     DASVLDMGCGAGHASFVAAQNVSTVVAYDLSAQMLDVVAQAAEVRQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSPGHPVRDIWL
                     QTVEALCDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFSSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVDAHKAA"
     gene            complement(5512..6312)
                     /locus_tag="ACHJWA_004320"
     CDS             complement(5512..6312)
                     /locus_tag="ACHJWA_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO4310795.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            6553..7467
                     /gene="yafC"
                     /locus_tag="ACHJWA_004321"
     CDS             6553..7467
                     /gene="yafC"
                     /locus_tag="ACHJWA_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFO4310796.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPVACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDREIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(7464..8267)
                     /gene="dkgB"
                     /locus_tag="ACHJWA_004322"
     CDS             complement(7464..8267)
                     /gene="dkgB"
                     /locus_tag="ACHJWA_004322"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFO4310797.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGLAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKEGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRENLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT         2252 a         2003 c         2051 g         2121 t
ORIGIN      
        1 aatataaatt tattctctgg aaatatattt aagctgaatg gttcgatatc attattgaat
       61 tttaatgaat gagacaacaa gcgagaatca cgcacttacg ccagagtaaa atcattgaac
      121 agagaaatgg tggaattcaa atacaaaaaa aaccgcaaaa ttaaaaatct tgcggttctc
      181 tgaactcatt ttcatgaggt gaatttggcg gaacggacgg gactcgaacc cgcgaccccc
      241 tgcgtgacag gcaggtattc taaccgactg aactaccgct ccgcgttgtg ttccgttggg
      301 aacgaggcga atattacgga ttgcctcacc cttcgtcaac ggtttttctc atcttttgaa
      361 tcatttgctg caaaaatcgc ccaagtcgct atttttagcg ccttttacat gtatttatgc
      421 ccgccagagg cagcttccgc cctttttctg caccagatcg agacgggctt catgtgctgc
      481 aagctcttca tctgtcgcaa aaacaacgcg taacttactt gcctgacgta caaggcgctg
      541 gattgttgct tcaccttgtt gctgttgtgt ctctccttcc atcgcaaaag ccatcgacgt
      601 ttgaccaccg gtcatcgcca gataaacttc cgcaaggatc tgggcatcga gtaatgcccc
      661 gtgcagcgtt cgtttactgt tatctatttc gtagcgggca cataacgcat cgaggctgtt
      721 gcgtttaccg ggaaacattt tcctcgccac cgcaaggcta tcggtgacct tacagaaggt
      781 attagtcttc ggaatatcgc gcttaagcaa cgagaactcg tagtccataa agccgatatc
      841 gaacgctgcg ttatggatca ccaactccgc gccgcggata tagtccatga actcatcggc
      901 tacttcggca aacgtgggct tatcgagcaa aaattcatcg gcaataccat gtacgccaaa
      961 ggcttccgga tccaccagcc gatcgggttt gagataaaca tggaagttat tgcccgtcag
     1021 gcgacggttc accacttcaa cggcaccaat ctcaatgatc ttgtggcctt catagtgcgc
     1081 accaatctgg ttcataccgg tggtttcggt atcgagaacg atctggcgtg taattgcagt
     1141 gctcatagcg gtcatttatg tcagacttgt cgttttacag ttcgattcaa ttacaggaag
     1201 tctaccagag atgcttaaac aggtagaaat tttcaccgat ggttcgtgtc tgggtaatcc
     1261 tggacctggg ggttacggcg ctattttacg ctatcgcgga cgcgagaaaa cctttagcgc
     1321 aggctacacc cgcaccacca acaaccgtat ggagttgatg gccgctattg tcgcgctgga
     1381 ggcgttaaaa gaacattgcg aagtcatttt gagtaccgac agccagtatg tccgccaggg
     1441 tatcacccag tggatccata actggaaaaa acgtggctgg aaaaccgcag acaaaaaacc
     1501 agtaaaaaat gtcgatctct ggcaacgtct tgatgctgca ttggggcagc atcaaatcaa
     1561 atgggaatgg gttaaaggcc atgccggaca cccggaaaac gaacgctgtg atgaactggc
     1621 tcgtgccgcg gcgatgaatc ccacactgga agatacgggc taccaagtcg aagtttaagc
     1681 ctgtggttta cgacattgcc gggtggctcc aaccgcctgg cgaattcgtg gcttgttttt
     1741 actctgtttc atcggattta gcgttaaagg aatagtccgt ttccgggcaa caataagttg
     1801 taagcaacca agcgcaggaa tatgcgcatt caaaagtttt cctccgtgtt tgttccacgg
     1861 gagaacgtgg aaacggctgg cgtgtagcac ttcaaaattc aacaaagaga gccagtccag
     1921 ttgccgcatc agagtaaaca tccggctgtt atagggcgag gttttgcgca ataccggcac
     1981 aagtttgcgt aatcccatta aactaatggg attgaagcca ctaatgacca gccagccatc
     2041 atcaatcaat acccgatcgg cttcacgcaa taaacgatgc ggatcggtgc accacggcaa
     2101 tgtatgtgcc agtagacaaa catcaacgga tttatcggca aaaggaagat gaagtgggtc
     2161 cgcctgcacc tggacaggca ttccttgcgc agaaacattc acttgatgag aaaccgcgca
     2221 cgcttcgcaa ttgatttctg cgcttaaatt gccaatctta agcagataaa aaccatacat
     2281 tttagtgaac cacgggttga gctggcgctc cagcgccttg cgataaagct ctccccaggg
     2341 caaatcgccc cagcgatcag gagccacgac agtttgaggg actcttgccg gtttcatcac
     2401 aaccttccgt ttcacactga gaggtaatct atgaatctta acagtattcc cgcctttgat
     2461 gacaattaca tctgggtttt gaatgatgaa gcaggtcgct gcctgattgt cgatcccgga
     2521 gacgcagagc cagtattaaa cgccattgcc gccaataact ggcaaccgga ggccatattt
     2581 ctcacccacc atcatcacga tcacgttggc ggcgtaaaag aactggtgga aaagtttccg
     2641 caaattgtgg tgtatggtcc acaagagaca caagataagg gaacaacaca ggtagtcaaa
     2701 gatggcgaaa ctgccttcgt tttggggcat gaatttagtg taattgccac gccgggtcac
     2761 actttaggac atatctgtta cttcagtaaa ccttatctat tttgcggcga cacgctgttt
     2821 tctggtgggt gtggtcggtt gtttgaaggg acagcatcac aaatgtatca atcacttaaa
     2881 aagttaagtg cgttacctga cgatacattg gtatgttgtg ctcatgaata tacgttatca
     2941 aatatgaagt ttgctttgag tattcttccg cacgatttgt ccataaatga ttattatcgt
     3001 aaagttaagg agttacgggc aaaaaatcaa ataacactac ccgtaattct gaaaaatgag
     3061 cggcaaatta atgttttttt aagaacggaa gatattgatt taattaatgt aattaatgaa
     3121 gaaacattat tgcaacaacc tgaagagcgt tttgcatggt taaggtcaaa gaaagatagg
     3181 ttctgataaa actttcttgt catcggctcc gttcgccgtt atgatcggtc gtcttttaag
     3241 caactattga cgcacacatg aaggcaaaag cgatattact cgcctctgtc ctgctcgtgg
     3301 gttgccagag taccggcaac gttcaacagc acgcacagag cctttctgca gctggtcaag
     3361 gggaagcagc aaagtttaca agtcaggcac gatggatgga cgatgggacg tctatcgcgc
     3421 cagatggtga cttgtgggct ttcattggcg acgagctaaa gatgggaatt ccggaaaatg
     3481 accggattcg cgaacagaaa cagaaatatt tacgcaataa gagctatctc cacgatgtaa
     3541 ctttacgggc agagccgtat atgtactgga tagccgggca agttaaaaaa cgtaacatgc
     3601 ctatggaact ggtactacta cccatagtgg agagcgcttt tgatcctcac gcaacgtctg
     3661 gcgccaatgc cgcaggcatc tggcagatca ttccgagcac ggggcgcaat tatggtttga
     3721 aacagacccg caattatgac gcgcgtcgcg atgttgttgc ttcaacaact gccgcgctga
     3781 atatgatgca gcgtctgaac aaaatgtttg atggcgactg gcttctgacc gtagcggctt
     3841 ataacagcgg cgaaggtcgg gtcatgaagg caattaaaac gaacaaagcg cgtgggaaat
     3901 ccacggactt ctggtcgtta ccgttgccgc aggaaacgaa gcagtacgtg cctaaaatgc
     3961 tggcattgag tgatattctc aaaaacagca agcgttatgg cgtacgtctg ccaacgaccg
     4021 atgaaagccg tgctctggcg cgtgtgcacc tgagtagccc ggttgaaatg gcgaaggttg
     4081 cagatatggc ggggatttcc gtcagcaagc tgaagacatt caacgctggc gtgaaaggct
     4141 ccacgctggg cgcaagtggt ccgcagtacg tgatggtgcc aaagaagcat gcagatcaac
     4201 tgcgtgaatc tctggcttca ggcgaaattg ctgctgtaca gtcgacgctg gttgccgaca
     4261 atacgccgct taacagccgt gtttacaccg tacgctctgg cgacacgctt tcaagtatcg
     4321 cttcacgtct cggcgtaagc accaaagatt tgcagcagtg gaacaaactg cgcggctcta
     4381 agctgaaacc aggccaaagt ctgacgattg gcgcaggtag tagcgcacag cggttggcaa
     4441 acaacagcga tagcattacg tatcgtgtgc gcaaaggcga ttcgctttca agcattgcta
     4501 aacgccacgg cgtgaacatc aaagatgtga tgcgctggaa cagcgatact gcgaatctgc
     4561 aaccaggcga taagctgacg ttgtttgtga aaaacaacag catgccagac tcctgacaaa
     4621 ccagataata gaaaggcacc gattcccccg gtgccttttt tatttatgcc gctttatgtg
     4681 catctaccat gatgatatca ctggtgaaag agccatcatt ctgcaaggca aaatacgttc
     4741 tcacctctgt cgatgcgctc tgttggtaaa tgcgaatagc gtctactaac gcttctggcg
     4801 tacgcattct cgcgacccat gaagaaaatt ccagcggtaa cttatctgta attaaattat
     4861 caactatcag attggcttca ttgattaacc tcaaccactc accgctggcg tagtttcgta
     4921 cgtgagaggt atcgcataat gcttctaccg tctgtaacca gatgtcgcgc actgggtggc
     4981 ccggagacat tacgtccatc acaatcagcc taccgccagg tttcaatatc cggttcactt
     5041 cacgcagtgc tgcaccaaca tcatgccagt gatgggcaga ataacggctg ataacaatat
     5101 caaatgcgtt atcggcaaat ggcagacttt cggcataccc ctggcgggtg gtgatatttt
     5161 tcagttgccg gacttcggca gcttgtgcca cgacatccag catttgggca gataagtcat
     5221 acgccaccac cgtgctcaca ttttgcgcag cgacaaagct ggcatgccct gctccgcagc
     5281 ccatatcaag tacacttgca tcaggataat cagccagaca caccgccagg agctgtaaat
     5341 ctcgtccgga tgcatgtacg gtactggtca gatattcgct ggcttgagag ctaaactgct
     5401 tttctacgtg gtcatggtgg gattgtgttg tcattgcact gtccttttgt tgtttgagta
     5461 taaaagggca gctccaaacg ttggtctgcc ctgtggcaga cctgacatac cttatttatc
     5521 aggcttgccg ggactgaatt caacgagtag cggattgtga tcggacgcgc gcgtaaccag
     5581 tacagaagct tcgctgacgt tcagaccacg gtagaaaaca aaatcgagcg ggcgaccaaa
     5641 cgcccgacgg cgctgatcat cggtaaaacg cacctgacgc agcgacattt cccgcgcaaa
     5701 acgatataac gcgttcatcc ttctacggct ccaagcattg aaatctcccg ccataatgac
     5761 cgggccgctg tggtgagcta tctgatcacc aataggaagt aactgcttac tataaacatc
     5821 cacgcccaga ctgaagttga cggcgtgtat attaaccacc attaacaggc gggtgtcagg
     5881 taatggatag accgtcacca gtgccgactt cgccagacgc aaaatgggtt ctcgttcacg
     5941 taacgggcag caatacactg gatgtgccgc cgaaagggtc attacgccag aaggatgttg
     6001 tggcagcacg aaagcgggta cctgatcggc ggcaagatag ttagcagtcg caaactgtac
     6061 caactctggc gttgtttgcg cttcctgcaa taacaccaga tgtgcgtctt tgccgtaatt
     6121 ctttaatacc gacaaccatt cagcgcgttg ctgtttgtat atgttccaca ccaggatccg
     6181 aatacgctct tcggtactta acggttctcc gggtggtaat gcctggccga tgctcgcaaa
     6241 agaccccggc ggtaagatcc tctccgcagg ttgtccggca acatagcgca tggcataggt
     6301 gttttttcgc acttttgact ttgaaacctc tattacgtca acccttccgc actcgggagg
     6361 gttctactgt tatagggatt ttagctcact ctttcaacga gcaattgtat attgttatgt
     6421 aagcaagtgc ttacaccggt cagaattttg ctcattattg agaacattaa atcggatgac
     6481 atatcacgca tcctgatggt ttaatgacaa ataaattcaa aatatctcat ctattttgca
     6541 acaggagcaa aaatgaaagc cacgtcggaa gaactcgcca tttttgtttc ggtcgtcgaa
     6601 agcggcagct ttagccgggc agcggaacaa ttagggcaag caaactcagc ggtaagccgg
     6661 gcggtgaaaa agctggagat gaaacttggc gttagcctgc ttaatcggac cacgcggcaa
     6721 cttagcctga cggaagaagg cgagcgttat tttcgtcgcg tacagtcaat tttgcaggag
     6781 atggcagcgg cagaatcaga aattatggag acgcgtaata caccgcgtgg actgttgcgc
     6841 atcgatgccg caactccagt ggtgctgcac tttctgatgc cgttgattaa gccttttcgt
     6901 gaacgctatc cggaagtcac tttatcgcta gtctcctccg aaacgattat taatttgatc
     6961 gaaagaaaag tggatgtcgc gatacgcgcc ggtacgttaa cggattccag cttacgtgcc
     7021 aggccgttat ttaacagtta tcgaaaaatt atcgcctccc ccgattatat ttcccgctac
     7081 gggaagccag aaacgatcga cgatttaaag caacatgttt gcctgggatt cactgaaccc
     7141 gcttccctca atacctggcc ggtagcctgt agcgatggac aattacatga ggtgaagtac
     7201 ggtttgtcat ccaatagtgg ggaaacactg aaacagcttt gcctgagtgg caacgggatt
     7261 gcgtgtttgt ccgactatat gatcgacaga gaaatcgctc gcggagaatt ggtggagtta
     7321 atggcagata aagtgttgcc agtggaaatg ccattcagtg ccgtctatta cagcgaccgt
     7381 gcggtaagta cgcgcatccg ggcttttatc gatttcctta gcgagcatgt aaaaacagct
     7441 cccggaggag ctgtcagaga ggcttaatcc cattcaggag ccagaccttc cgggctaacc
     7501 aggcggtcgt tgcaatccag tgcggcgatc gcttttttat cttcggcatc aagctgtaaa
     7561 ttttgtgcct taagattact ctccaggttt tcacgtttag tagaagaagg aattactgag
     7621 taaccttccc ccatagccca cgccagaatc acttgtgccg gagtcgcatt gtgtttagct
     7681 gcgatacgag caataacctc atctttcagg gccttaccat acgccagcgt catataggaa
     7741 gtaatatgga tgccgtgctg tttagcccag gcaaccactt tacggttttg cagataagga
     7801 gagagttcaa tctggttagt agcgatgttt tcagcgccaa cagcagcaat cgccttttcc
     7861 atcaatggga tcgtgaagtt ggaaataccg atctcacgcg tcaacccttc ttttttggct
     7921 tccagcagcg cctgcataaa ctcttcaaca gagacttcat cgtttggtga cggccagtgg
     7981 attagcgtca gatcaacata atcggtacgc aatttttgca ggctctcttt tagactcggg
     8041 atcaatttgt ctttgctgag attttcaatc cagattttag tggtgatgta gagttcatga
     8101 cgtggcacgc cactttctgc aatcgccaga cctactgcgg cttcgttatc atagatttgt
     8161 gcagtatcaa ttgcgcgata accaagttca agcgccgttt tcacagatga aataacaacg
     8221 tcgtctttca gacggaaagt acctaaacca aatgcaggga tagccataat atgcctcttt
     8281 taatgctatt ttcgttaact gaggtgatta tgggctttag aatgttgcgg aaaaagggcg
     8341 taaaacgcag aggatttttg cgattttagc aataatagaa atgtagacga aaaaaaacct
     8401 cgagcgataa ctcgaggctt cttaata
//
LOCUS       DAZFAB010000153         8388 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZFAB010000153 DAZFAB010000000
VERSION     DAZFAB010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8388
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.153"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(192..1385)
                     /gene="tyrB"
                     /locus_tag="ACHJWA_004323"
     CDS             complement(192..1385)
                     /gene="tyrB"
                     /locus_tag="ACHJWA_004323"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFO4310798.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAVPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLAMLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(1638..2717)
                     /gene="alr"
                     /locus_tag="ACHJWA_004324"
     CDS             complement(1638..2717)
                     /gene="alr"
                     /locus_tag="ACHJWA_004324"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFO4310799.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKVGEPVGYGGTWISERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(2770..4185)
                     /gene="dnaB"
                     /locus_tag="ACHJWA_004325"
     CDS             complement(2770..4185)
                     /gene="dnaB"
                     /locus_tag="ACHJWA_004325"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFO4310800.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISSYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4268..5251
                     /locus_tag="ACHJWA_004326"
     CDS             4268..5251
                     /locus_tag="ACHJWA_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFO4310801.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(5417..5659)
                     /gene="pspG"
                     /locus_tag="ACHJWA_004327"
     CDS             complement(5417..5659)
                     /gene="pspG"
                     /locus_tag="ACHJWA_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFO4310802.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(5793..6830)
                     /gene="dusA"
                     /locus_tag="ACHJWA_004328"
     CDS             complement(5793..6830)
                     /gene="dusA"
                     /locus_tag="ACHJWA_004328"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFO4310803.1"
                     /translation="MHDNHEAQKINQTSVMPEKTGVYWNSRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            6919..8016
                     /locus_tag="ACHJWA_004329"
     CDS             6919..8016
                     /locus_tag="ACHJWA_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4310804.1"
                     /translation="MAYYNIEKRLKSDGTPRYRCNVIIKEKGVITYRESKTFPKHAHA
                     KTWGTQKVMELDLYGIPSSNAVDGLTVRDLLHKYLNDPNAGGKAGRTKRYVLELLMDS
                     DISAIKLSELTENDVIEHCRLRNNAGAGPATVSHDVSYLGSVLDAAKPVYGINYTSNP
                     AKSARPYLLKLGLIGKSNRRNRRPASDELDMLIEGLQQRSTHKCSKIPFVDILKFSVW
                     SCMRIGEVCRLRWEDLDQEQKSILVRDRKDPRKKEGNHMKVALLGEAWDIVQRQPKKS
                     EFIFPYNSTSVTAGFQRVRSKLGIKDLRYHDLRREGASRLFEAGFSIEEVAQVTGHRS
                     LNVLWQVYTELYPKSLHNRFEELQKSRNKTS"
     gene            8078..8326
                     /locus_tag="ACHJWA_004330"
     CDS             8078..8326
                     /locus_tag="ACHJWA_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO4310805.1"
                     /translation="MRIEICIAKEKMTKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWKSADEWFVH"
BASE COUNT         2231 a         2147 c         2031 g         1979 t
ORIGIN      
        1 tactccacac gaaaaaaaca gggaaaggaa ttccagataa ttgggataaa cttaacaata
       61 aaagaactaa caacgtttat tatctctcac gtagaacgat ggtatcaaaa aaatcatcta
      121 taaagaaaga gtgaaagcga cgacagttta atttcactgc aggctgggta attccagcct
      181 gctttcctgc attacatcac cgcagcaaac gcctttgcca cacgttgcac atttgccgta
      241 tttaacccgg cgacacacat gcgaccgctg gcgatgagat agacaccaaa ttcttcacgt
      301 agtcggtcaa cctgagcggc acttaaaccg gtataactga acatgccgcg ctgattaagc
      361 agataatcga aattgcgttc tggcatctct gtgcttaata ccttcaccag ttcctgacgc
      421 attgccagaa tgcgagtacg catctcttct acttccgcca gccagctggc tttcaatgcc
      481 tcgtcattca gcactgcagc caccacctgc gcaccaaaat tcggcgggct ggagtagttg
      541 cggcgaactg ttgctttcaa ttgccccagt acgcggcctg cagcttcggc atcttcacac
      601 agaacagaaa gtccgccgac gcgctcgccg taaagggaga aaattttcga gaacgaattg
      661 ctcaccagag cgggtaatcc agcgctggca atggcgcgaa tggcgtaggc atcctcttcc
      721 ataccggcac caaatccttg ataggcaata tcaaggaatg ggataagctc gcgggctttg
      781 agaatttcaa tcaccgcatc ccactggtca ttagtgagat cggcacccgt tgggttgtgg
      841 caacatggat gcagcaacac aatactgcgg gcaggtaatg ttttcagcat cgccaacagg
      901 tcattaaagc gcacgccgtt agtcgcttcg tcataccagg ggtaagtgct tacttcgaat
      961 ccagccccgg cgaatattgc tacgtggttt tcccaggtag gatcgctgac ccagacgcct
     1021 gattccggga agtagcgttt caggaaatcc gcgcccactt tcaatgcccc tgagccgcca
     1081 agggtttgaa tggttgctac gcgctgttgt tgcagtaccg gatggtcggc accaaacagc
     1141 agcggcgcaa tggcatggcg atagctgtta agcccttcca tcggtaaata aagcgaagcg
     1201 ccatgaggca ccgcattcag gcgcgcttcc gcctccgcca cggctttcaa ttgtggaata
     1261 attccgtctt cgttgtagta cagaccgata cttaaattca ctttgtcgct gcgagggtct
     1321 tctttaaaac gctccataag cgtaagaatc gggtcgccag cgtaggcgtc aactttttga
     1381 aacacgcgat ggttctccag gtttacgggc aggtggttaa aacacaataa accggaagaa
     1441 ggcgaagatc gagtggatgt tcaggggcga acggcaatta gcaacagagt gagactcatg
     1501 acaaacgtac atccgccaga ggcacgacct ttataagagc atgaaagaat atcaacttat
     1561 tgaattggta ggattttatt ggccggataa ggcattcacg ccgcatccgg cacagacaat
     1621 caaatattac agaacgatta atccacgtat ttcatcgcga cccttgaagt caggcgcgta
     1681 ataagttcgt aagcgcttac tttcgtcatt tcagcgatac gttctacggg caaaccttca
     1741 ccccacaaaa tgaccgggtc cccggcttta tcctgtgcct gtggacctaa gtctacgcag
     1801 atcatatcca tcgccacgcg cccgacaatc ggcacttcgc gaccgttcac cagcactggc
     1861 gtaccggacg gcgcggcgcg cggataacca tcgccatagc ccatcgcaac tacaccaaga
     1921 cgagtatcac gttcgcttat ccaggttcca ccataaccga caggctctcc gactttatgc
     1981 tcacgcacgg caatcaggct ggaggttagt gacatcactg gctgacagcc aaaatcggca
     2041 ccggtggagc gatcttccag cggcgagacg ccataaagaa tgatgcccgg gcgcacccag
     2101 tcaaaatgcg actgtggcca cagcagaatg ccacccgacg cggcaatgga acgttgacct
     2161 ggtttgcctt cgcaaaaggt attaaagata gcgagttgtt tctcggttgc gccacatttt
     2221 ggttcatccg cgcgcgcaaa atggctgacg atattcaccg gctgccgaac gtttttgcac
     2281 tgggtcaggc gatgataaaa cgcctcagcc tgttccggcc ttacgcccag acggtgcata
     2341 ccggtatcga gtttcatcca gacggtaacc ggctcgtcca ggctagcctc ttccagcgca
     2401 gccagctgtt cttcgttatg cacggcggta tgaaaatgtt gcgcagaaat cgtcggcaga
     2461 tctctggcat caaaaaagcc ttcgagtaac agtacaggtt tggtgattcc ccccgcacgc
     2521 agtcgcagag cttcttcgag acgggctacg ccaaaggcgt cagcatcggg gagcgttcgc
     2581 gcggtctcaa gaagaccgtg accataagcg ttcgctttca ccaccgcaac cattttactg
     2641 gcaggtgcca gttcacgaag acgttgcagg ttgtgtcgca gagcgcggcg gttaatcaca
     2701 acagttgccg cttgcatttg tgttccttga taagtgtttg ctttaattac ctaattcata
     2761 aaataattat tattcgtcgt cgtactgcgg ccccgcatag ttgtcgaagc gcgaccattg
     2821 accgttaaag gtcaggcgta ccgtcccgat tgggccgtta cgttgtttac cgataataat
     2881 ttccgcgatg ccttttaaat cactgttttc gtgatacacc tcatcacgat agataaacat
     2941 gatcaagtcc gcatcctgct cgatagagcc agattcacgc aggtcggagt tgaccggacg
     3001 tttgtcggca cgttgttcca gagaacggtt caactgggac agcgccacca ccggcacgtt
     3061 cagttctttc gccagtgctt tcagcgagcg ggagatttct gcaatttcca gcgtacggtt
     3121 atcggaaagt gccggtacgc gcatcagttg caggtagtcg atcatgataa gcccgatgcc
     3181 gccgtgttca cgggcaatac ggcgtgcgcg ggaacgcact tccgttggcg tcaggccgga
     3241 ggagtcatcg atatagatat tgcgtttttc gagcaaaata cccatggtgc cggaaatgcg
     3301 cgcccagtct tcatcatcga gctgcccggt acggatttta gtctggtcaa cgcgcgacag
     3361 cgacgccaga gaacgcatca tgatctgttc tgatggcatc tccagcgaga aaataagtac
     3421 cggtttatcc tgcaacatcg ccgcgttttc gacgaggttc atcgcaaatg ttgttttacc
     3481 catcgacgga cgcgcggcga cgatgatcaa atccgacggc tgcaagccag cggttttttt
     3541 gttgagatca tcataaccgg tgtttacccc ggtaacgcca tcgtgtggct gctgaaataa
     3601 ctgctcaata cgcgccacgg ttgcgtcgag cacatcggcg atgttcttcg gcccttcgtc
     3661 tttgttcgca cgactttcgg caattttaaa gacgcgggat tcagccagat ccagcagatc
     3721 ttcgctggta cgcccctgcg gatcaaaacc agcttcggca atctcattcg caaccgagat
     3781 catctcacgg acaacggcac gttcacgcac gatgtccgca taggaactga tgttcgcagc
     3841 acttggcgta ttttttgaca actctgccag ataagcaaaa ccaccgacgc tatcgagttg
     3901 cccctggcgt tccagcgatt ccgcaagggt aatcagatcg ataggactac cgctttcctg
     3961 caaacgcgcc atttcagtaa agatatgacg gtgtgggcgg gtgtaaaaat cgtctgctac
     4021 cacacgctcg gctacatcat cccagcgttc gttatccagc attaaaccgc ccaacaccga
     4081 ctgctccgct tcgatcgagt gcggaggcac tttcagcccg gcaacttgtg gatcgcgttc
     4141 gcggggttca gcctgctgtt tgttgaaggg tttatttcct gccatagtga atggagttac
     4201 cgagataaag aatgggtcga aactttacca tatgaagcag accctgacga tacgttctgg
     4261 aggacacatg gcaacacgaa ttgaatttca caagcacggt ggcccggaag tacttcaagc
     4321 cgtagagttc actcctgccg atccggcgga gaatgaaatc caggtcgaaa ataaagccat
     4381 cggcatcaat tttatcgaca cgtatatccg cagcggcctt tacccgccgc catcactacc
     4441 cagcggatta ggtaccgaag cggcaggcat cgtgagtaaa gtcggcagtg gtgtaaagca
     4501 tattaaggca ggcgatcgtg tagtctatgc gcagtcggcg ttaggcgctt acagctctgt
     4561 gcataacatt aatgcggata aagcggcgat tctgcctgcg gcaatttctt ttgagcaagc
     4621 tgcagcatct ttcctgaaag gcttaacggt ttattatctg ctgcgcaaaa cctatgaaat
     4681 taaacccgat gaacagttcc tgttccacgc agcagctggc ggcgttggct tgattgcctg
     4741 ccagtgggca aaagccctgg gcgcgaaact tatcggcacc gtaggaaccg cgcaaaaagc
     4801 gcagagcgcg ctaaaagcgg gcgcgtggca ggttattaac tatcgtgaag agaatctggt
     4861 cgagcggtta aaagagatca ccggtggtaa aaaagtgcgc gtagtgtacg attccgtggg
     4921 cagagacacc tgggaacggt cgctggattg cctgcaacgc cgcggcttaa tggtgagttt
     4981 tggcaactca tcaggtgcgg ttaccggtgt gaacttaggc attctcaatc agaaaggctc
     5041 gttgtatgtg acacgccctt ccctgcaagg ctatatcacc acgcgggagg aattaaccga
     5101 ggccagtaat gaactgttct ctttgattgc cagcggtgtg attaaggtcg atgtcgccga
     5161 gcagcagaaa tatccgctga aggatgcgca gcgtgcgcat gagattctgg aaagccgagc
     5221 gacgcaaggt tccagcctgt tgattccata aaagaaatag ggcttccacc tgggaagccc
     5281 tttcttttta tagttcggct gtatgtaggg tacagcacga tgaatctgtt agaggcgcaa
     5341 tagtgacaga tttgattatc aattcctatt ttgttctaag gataaaacct taggttgtga
     5401 tcatccgcac aatcccttag taacgccagc ggtcataacg ctgatatttc ggcacttttg
     5461 gtgctttaat cgccttaata acccacacca ccgcaatcgc caggagtaac cacggcagca
     5521 acttaatcat caatgccagc ataccgccga ggaacataat ggccgtcgcc acaaccagcg
     5581 cggcgataat gcccagcaac gaaacgccgg tgaccatcaa catgacaaaa aagccaatca
     5641 caaaaagtag ttccagcatg atgctctccc aaatatgaaa tctcttgcag gcattacaag
     5701 aatcatgcca aaaataatct attgatttaa cagcaaaacg ccccgcgacg gtgcgcaggg
     5761 cgtggtgaat ttgactactt tttggtgaaa agttaacgct tatccgccac cagtttgagc
     5821 gcgtgttcca gcacgttaat gtctgccccc gctttatggg cattttcact taaataacgc
     5881 cgccactgcc gcgcgccagg aataccctgg aacaagccca acatatgccg ggtaatatgg
     5941 ccaagatacg tcccctggct gagttcacgc tcaatgtacg gatacatggc gcgcactacc
     6001 gccaccggat cggcatcggt atccgaggaa ccaaagatct ctcggtctac cgccgccaga
     6061 atacccggat tctgatacgc ttcgcgcccg accatcacgc catccatatg ttgcaaatgc
     6121 gctttagcct cttccagcga cttgatacca ccgttaatcg acatcgtcag atgcggaaag
     6181 tcacgcttca gttgatacac acgcggataa tcgagcggcg ggatttcacg gttttctttc
     6241 gggcttaacc ccgaaagcca ggctttacgt gcgtggatga tgaacatctc acactcgcct
     6301 ttgccggaaa cggtgttgat gaaatcgcag agaaattcat agctgtcctg gtcatcgatg
     6361 ccaatacgcg ttttcaccgt caccggaatc gacaccacat cgcgcatcgc tttcacgcag
     6421 tcggcaacca gctgcgcatt acccatcaga cacgcaccaa acatgccgtt ctgcacccgg
     6481 tcagacgggc agccgacatt caggttgatc tcatcatatc cacgcgcttc tgccagcttc
     6541 gcacactgcg ccagcgccgc cggatcgcta ccgccgagtt gcaacgctac cggatgttct
     6601 tcttcactgt acgccaggta atcaccttta ccgtgaataa tcgcccctgt agtcaccatt
     6661 tcggtataca gcaacgtatt gcgggaaagc agacgcaaga aatagcggca atgtctgtcc
     6721 gtccagtcga gcattggtgc aacgctaaac cgagaattcc agtaaacacc agttttttca
     6781 ggcatcacgc tggtttgatt aattttttgt gcttcatgat tatcgtgcat ttttgaacat
     6841 ttcaggctat ttttctcgcg ttaggttccc gcacaggttc ccacgtttta tgggaacccg
     6901 aaataacgag gtcgtgtaat ggcgtactat aacatagaga aacgactaaa atccgatggc
     6961 acaccacgct atcgctgtaa tgtgattatc aaagaaaaag gtgttatcac ttacagggaa
     7021 agcaaaacat tccctaaaca tgctcatgcc aaaacatggg gcacacagaa agtgatggaa
     7081 ttagatctat atggcattcc atcatcaaat gcagttgacg gacttacagt ccgtgactta
     7141 ctacacaaat atttaaatga cccaaatgcc ggaggtaaag caggccgtac taaaagatat
     7201 gtgctggaac tgcttatgga tagtgacata tccgcgatca aactatctga actgacagaa
     7261 aatgacgtaa ttgaacattg caggctaaga aacaacgctg gtgcaggccc agcaacagtc
     7321 agccacgatg ttagttatct tggcagtgtt ctggatgcgg caaaacctgt atacggaatc
     7381 aattacacat caaacccggc gaaaagcgct cgtccatatc tacttaaact cggtttgatt
     7441 ggtaaatcaa accgtcgtaa tcgtagacca gcatctgatg aacttgacat gctcattgaa
     7501 ggccttcaac aacgatctac tcataaatgc tcaaaaattc cgttcgttga tatcctcaaa
     7561 ttttctgtgt ggtcctgtat gcgaatcgga gaagtatgcc ggttacgatg ggaagatctc
     7621 gaccaagaac aaaaatctat actagtaaga gataggaaag atccacgtaa aaaggaaggt
     7681 aaccatatga aagttgcctt gcttggggaa gcctgggata tcgtccagcg acaaccaaaa
     7741 aaatcagaat tcatttttcc atataacagc acttctgtta ccgcaggatt tcagagggta
     7801 agaagcaaat taggtattaa agatctgcga tatcatgatt tgcgtagaga aggggcaagt
     7861 cgcttatttg aggctggttt tagtattgag gaagtcgctc aagttacagg gcatcgttca
     7921 ttaaacgtgc tatggcaggt atataccgaa ctgtatccga aatctttaca taatcgtttt
     7981 gaagaactcc aaaagagcag aaacaagacc tcttgacact gtttatttat acagataaaa
     8041 ataacactgt atacaaacac agtatagagg gatttttatg cgtattgaaa tctgcatagc
     8101 caaagagaaa atgactaaaa tgccaaacgg tgctgtggat gcgttaaagg aagaattaac
     8161 tcggcgcatc agtaagcgtt atgacgatgt agaagtgatc gtaaaagcca ccagcaatga
     8221 tgggctttct gttacacgca ctgcagataa ggattctgca aaaacatttg ttcaggagac
     8281 tctgaaagat acctggaagt ctgctgacga gtggtttgtt cactaattag cacgtaaatc
     8341 ggtaacggct ggaaatcatc caagactagc actatcgaaa gttcacca
//
LOCUS       DAZFAB010000154         8254 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZFAB010000154 DAZFAB010000000
VERSION     DAZFAB010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8254
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.154"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(8..214)
                     /locus_tag="ACHJWA_004331"
     CDS             complement(8..214)
                     /locus_tag="ACHJWA_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFO4310806.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            527..616
                     /gene="kdpF"
                     /locus_tag="ACHJWA_004332"
     CDS             527..616
                     /gene="kdpF"
                     /locus_tag="ACHJWA_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFO4310807.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALIHAEAF"
     gene            616..2289
                     /gene="kdpA"
                     /locus_tag="ACHJWA_004333"
     CDS             616..2289
                     /gene="kdpA"
                     /locus_tag="ACHJWA_004333"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFO4310808.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLAWLINDIPLPGTTGVER
                     VLFSALGVSNHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALVIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2312..4360
                     /gene="kdpB"
                     /locus_tag="ACHJWA_004334"
     CDS             2312..4360
                     /gene="kdpB"
                     /locus_tag="ACHJWA_004334"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFO4310809.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSVAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVVATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAVTYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4369..4941
                     /gene="kdpC"
                     /locus_tag="ACHJWA_004335"
     CDS             4369..4941
                     /gene="kdpC"
                     /locus_tag="ACHJWA_004335"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFO4310810.1"
                     /translation="MRGLRPALSTFIFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTDNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPNASTSVPVELVTASASGLDNNITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            4934..7618
                     /gene="kdpD"
                     /locus_tag="ACHJWA_004336"
     CDS             4934..7618
                     /gene="kdpD"
                     /locus_tag="ACHJWA_004336"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFO4310811.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRLAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RKLALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            7615..>8254
                     /gene="kdpE"
                     /locus_tag="ACHJWA_004337"
     CDS             7615..>8254
                     /gene="kdpE"
                     /locus_tag="ACHJWA_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFO4310812.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPSHF"
BASE COUNT         1719 a         2158 c         2419 g         1958 t
ORIGIN      
        1 tcagcaatca gtaaaaatca ccggttgcct tttcggcctg atccatccaa accggtttat
       61 cgctggtttt cgaccagacg cgatgcaggt agctgtaaaa gcgggcgcgg tctttccaga
      121 aaagcatgaa aggcagcgcc agaacgcccg ctgcaaccgc ataagtacgg cgtaaaaaga
      181 taagccatgc aggatattct ctgtagagtt ccatactttt ctcccccttt tgtatctacc
      241 cggtgaatag caccggaaaa atgaatttgt ttatctgatg aaaatagtac cgccttttgt
      301 gtaattttac tactcatccg accacttatt tttgcttatt gatggtttat ttacattcat
      361 cctgtaatta agttacacaa aagttaaatt aatactaaac attagttaaa tcatggcttt
      421 tgccattttt atactttttt tacaccccgt ccgcagattt ttgcgaaatc tttgcagcca
      481 gaattctacc cttccggtat cacttttagg ccactggagg tgcactgtga gtgcaggcgt
      541 gatagccggc gtgttgctgg tgtttttatt actgggttat ttggtttatg ccctgatcca
      601 tgcggaggca ttctgatggc tgcgcaaggg ttcttactga ttgccacgtt tttactggta
      661 ttaatggtgc tggcgcgtcc cttaggtagc gggctagcgt ggctgattaa tgatattcct
      721 cttcctggta cgacgggcgt tgaacgcgta ctctttagcg cgcttggcgt ctctaaccat
      781 gagatgaact ggaagcagta tctctgtgcc attctcggcc tgaacatgct ggggctggct
      841 gtgctgtttt ttatgttgct cggtcagcac tatctgccgc ttaatccaca gcagttgccg
      901 gggctgtcgt gggatctggc gctgaatacc gccgtcagct ttgtcacgaa taccaactgg
      961 caatcttata gcggtgaaac cacgttgagc tatttcagcc agatggcggg cttaacggtg
     1021 caaaactttc tttctgccgc cagcgggatt gcggtgattt ttgccctcat tcgtgcattt
     1081 acccgccaga gcatgagcac actcgggaat gcctgggtcg atctgctacg catcacgtta
     1141 tgggtgctcg tccctgtggc gttggtgatc gcactgtttt ttattcaaca aggtgcgctg
     1201 caaaactttc tgccctatca ggcagtgaat accgttgaag gagcgcaaca gctgttacca
     1261 atgggtccgg tggcttctca ggaagcgatc aagatgctcg gcactaacgg cggtggtttc
     1321 tttaatgcca actcgtcgca tccgtttgaa aacccaaccg cactgaccaa cttcgtgcag
     1381 atgctggcga tcttcttgat cccaacagcg ctgtgctttg cctttggtga agtgacgggc
     1441 gatcgccgtc aggggcgcat gttgctgtgg gcgatgtcag tgatttttgt catctgcgta
     1501 ggcgtggtga tgtgggcaga agttcagggt aatcctcatc tgctggcgct cggcgcagac
     1561 agcagcatca atatggaagg taaagagagc cgtttcggcg tgctggtcag tagcctgttt
     1621 gcggtcgtga ctacggcggc gtcctgtggc gcggtgattg caatgcatga ttcgtttacc
     1681 gctctcggtg gcatggtgcc gatgtggctg atgcaaattg gtgaagtggt attcggcggt
     1741 gtcggttctg gtctttacgg catgatgctg tttgtcctgc tggcggtgtt tattgccggg
     1801 ctgatgattg gtcgtacacc ggaatatctg ggtaaaaaaa tcgacgtacg tgagatgaaa
     1861 ctgactgcac tggcgattct ggtcaccccg acgctggtgc tgatgggcgc ggcgctggca
     1921 atgatgaccg acgccggacg tagcgccatg ctcaaccctg gcccgcatgg ttttagcgaa
     1981 gtgctgtacg ccgtgtcatc cgccgctaac aacaacggca gcgcctttgc cggattaagc
     2041 gccaactctc cgttctggaa ctgtttactg gcgttctgca tgtttgtcgg tcgcttcggg
     2101 gtgattatcc cggtgatggc aattgccggt tcgctggtga gtaaaaagag ccaacccgcc
     2161 agctccggca cgctgccaac gcacggcccg ctgtttgttg gcctgttaat cggcaccgtg
     2221 ttgctggttg gcgcactgac ctttatccct gccctggcgc ttggtccggt ggcggaatat
     2281 ctctcctgat gatattgagt gagcactgaa tatgagtcgt aaacaactgg cgctattcga
     2341 accaacactt gtcgttcagg cgctgaaaga agcggtgaaa aaattaaacc cgcaggcgca
     2401 atggcgcaat ccggtgatgt ttatcgtctg gatcggcagt ctgctgacca cctgtattag
     2461 catcgcgatg gcaagcggcg cgatgcctgg caatgcgctg tttagcgtgg ccattagcgg
     2521 ctggctgtgg gtcaccgtac tgttcgctaa tttcgccgag gcgctggcag aaggccgcag
     2581 taaagcgcag gccaacagcc tgaaaggggt gaaaaaaacg gcctttgccc gcaagctgcg
     2641 tgagccgaaa tatggcgctg cggcggacaa agttcccgcc gatcaactgc gtaaaggcga
     2701 tatcgtactg gtagaagcag gcgatattat cccctgcgat ggtgaagtta ttgaaggcgg
     2761 cgcatcggtc gatgaaagcg ccatcaccgg ggaatcggca ccggtgatcc gtgaatccgg
     2821 cggcgatttt gcctccgtca ccggcggcac gcgtattctt tctgactggc tggtgattga
     2881 gtgtagcgtt aaccccggcg agacatttct ggatcggatg atcgcgatgg tggaaggcgc
     2941 acagcgacgc aaaacgccga acgagattgc cctgaccatc ctgctgattg ccctgactat
     3001 cgtcttttta ctggcaaccg ccacgctgtg gccgttttcc gcgtggggcg gtaatgcagt
     3061 cagcgtaacg gtactggtgg cgctgctggt ctgtctgatc ccaaccacta ttggcggcct
     3121 gttgtcagcg atcggcgtcg ccgggatgag tcggatgctg ggcgcgaatg tggttgccac
     3181 cagcggacgt gcagttgaag cggcaggtga cgttgacgtt ctgctgctgg ataaaaccgg
     3241 caccatcaca ctcggtaacc gtcaggcatc ggagtttatc cctgcgcagg gcgtggatga
     3301 aaaaacgctg gctgacgccg cacaactggc ttcgctggct gatgaaacgc cggaaggccg
     3361 cagtattgtg atcctcgcca agcagcgttt taacctgcgc gagcgcgatg tgcagtcgct
     3421 ccatgccacc tttgtaccgt ttactgcgca aagccggatg agcgggatca atatcgacaa
     3481 ccgcatgatc cgtaaaggtt ctgtcgatgc cattcgccgc catgttgagg ctaacggtgg
     3541 tcacttccct gccgatgttg atcaaaaagt cgatcaggtt gcgcgtcagg gggccacacc
     3601 gctggtagta gtagaaggtt ctcgtgtgct gggcgttatt gcgttgaaag atatcgtcaa
     3661 aggcggcatt aaagaacgct tcgcccaact gcgcaaaatg ggtattaaaa cggtgatgat
     3721 taccggcgat aaccgtctga ctgccgccgc gatagctgcg gaagcgggcg tcgatgattt
     3781 tctcgccgaa gcgacaccgg aggccaagct ggcattgatc cgtcagtatc aggcggaagg
     3841 ccgtttggta gcaatgaccg gcgacggcac caacgatgct ccggcgctgg cgcaggcaga
     3901 tgtcgcggtg gcgatgaact ccggtactca ggcggcgaaa gaagcgggca atatggtgga
     3961 tctcgactct aacccgacca agttgatcga ggtggtacat attggcaaac agatgctgat
     4021 gacccgtggc tcgctgacca ccttcagcat tgccaacgat gtggcgaaat acttcgccat
     4081 tattccggcg gcattcgcgg taacgtatcc acagttaaat gcgctgaaca tcatgcgcct
     4141 gcattcgccc gactccgcca ttctcagtgc ggtcattttc aacgccttga ttatcgtctt
     4201 tttgattccc ctggcgttaa aaggcgtgag ttataaaccg cttaccgctt ctgccatgtt
     4261 gcgccgtaac ttatggattt acggtctggg tgggctgctg gtgccgttta ttggtatcaa
     4321 agtcattgat ttactactga ccgtttgcgg tctggtgtga ggtttaccat gcgtggatta
     4381 cgtccggcat tatcgacatt tatctttctg ttattgatta ctggcggtgt ttacccgctg
     4441 ctgaccaccg cgctggggca atggtggttt ccctggcagg ccaacggttc gttgatccgt
     4501 gaaggtgata cggtgcgggg ttcggcatta atcgggcaga attttaccga caacggctat
     4561 tttcatggtc gcccgtcggc aacggcagaa atgccttata atccacaggc ttctggcggg
     4621 agcaatctgg cggtcagtaa ccctgagctg gataaactaa tagccgcacg cgttgctgca
     4681 ttacgggccg ctaacccgaa tgccagcacg agcgttccgg ttgaactggt gacggcgtcg
     4741 gcaagcgggc tggacaataa catcaccccg caagcggcgg cctggcagat cccacgcgta
     4801 gcgaaagcgc gtaatctcag cgttgaacag ctcacgcaac tgatcgcaaa atacagccaa
     4861 caaccactgg tgaaatatat cggccagccg gtcgtcaaca ttgtcgaact caatctggca
     4921 ctggataaac ttgatgaata acgaaccctt acgtcccgac cccgatcgtc tgctggaaca
     4981 aactgccgcg ccgcatcggg ggaagctgaa agttttcttc ggtgcctgcg caggcgtcgg
     5041 gaagacctgg gcgatgctgg cagaagccca gcgactgcgg gcgcaagggc tggatattgt
     5101 ggttggcgtg gtagaaaccc acgggcggaa agataccgcc gccatgctgg aagggctggc
     5161 tgttctgccg ccaaaacgcc tggcgtaccg tgggcggcac atcagcgagt ttgatctcga
     5221 tgccgccctc gcccgccgtc cggcgctgat cttaatggac gaactggcgc acagtaatgc
     5281 gccaggttct cgtcatccta aacgctggca ggatatcgaa gaactgctgg aagctggcat
     5341 tgatgttttc acaaccgtca acgttcagca tctggaaagt ctgaatgatg tggtcagcgg
     5401 cgtcaccgga attcaggtac gggaaaccgt gcccgatcct tttttcgatg ccgccgacga
     5461 cgtggtgctg gtggacttgc ccccggacga tctgcgccag cggctgaaag aaggcaaagt
     5521 ctatattgcc gggcaggcgg agcgcgccat tgaacatttt ttccgcaaag gcaatctgat
     5581 cgccctgcgc aaactggcac tgcgccgtac tgccgatcgc gttgatgagc aaatgcgcgc
     5641 ctggcggggg catcctggcg aagagaaagt ctggcacacg cgcgacgcga tccttttatg
     5701 catcggccat aacaccggca gcgaaaaact ggtccgcgcg gcggcgcggc tggcgtcacg
     5761 gctggggagc gtctggcacg cggtgtatgt tgaaacccct gccctgcatc gcttaccgga
     5821 gaaaaaacgt cgggcaattc tcagcgcctt acgtctggcg caggagctgg gcgcggagac
     5881 ggcaacactt tctgatccag cggaagagaa agcggtagtg cgttatgccc gtgaacataa
     5941 tctcggcaag attattctcg gtcgcccagc ctcgcgccgc tggtggcgtc gggaaacgtt
     6001 tgctgaccga ctggcgcgca tcgcccccga tctcgatcag gtgctggtcg cgcttgatga
     6061 accacccgcc cgcacgatta acaacgcgcc ggatagccgc tcttttaaag acaagtggcg
     6121 ggtacagatt cagggatgcg tggttgccgc cgcgttatgc gccgttatca ccttaattgc
     6181 catgcagtgg ctgatggcgt ttgatgccgc caacctggtg atgctgtatc tacttggcgt
     6241 ggtggtggtg gcgctatttt atggacgctg gccttcggtg gttgctaccg tcattaatgt
     6301 ggtgagtttc gatctctttt ttatcgcccc acgcggcacg ctcgccgtct ctgatgtgca
     6361 atatctgctg accttcgcgg tgatgttaac cgtcgggctg gtgatcggga accttactgc
     6421 gggtgtgcgt tatcaggcgc gggtagcccg ttaccgcgag caacgcacac ggcacttata
     6481 tgaaatgtcg aaagctctgg cggtgggccg cagtccgcag gacatcgccg ccaccagcga
     6541 acaatttatt gcctccacgt ttcatgcccg cagtcaggtg ttgttgcccg atgacaacgg
     6601 taaattgcag ccgttaacac atccgcaagg aatgacgccg tgggacgatg ccatcgcgca
     6661 gtggagtttt gataaaggcc tgcctgcggg tgcgggcacc gacacgttac ccggcgtgcc
     6721 gtaccagatt ttgccgttaa aaagcggtga gaaaacctac gggctggtgg tggtggaacc
     6781 ggggaatctg cgtcagttga tgatcccgga acagcagcgc ctgctggaga cgtttacgct
     6841 gttagtcgcc aatgcgcttg agcggctgac gctgaccgcc agcgaagaac aggcgcggat
     6901 ggcaagcgaa cgtgaacaga tccgcaacgc cctgctggcg gcgctttcgc atgatttacg
     6961 cactccgctt acggtgctgt ttggtcaggc agaaatctta acgctcgatc tggcaagcga
     7021 aggatcaccc cacgcccgcc aggccagcga gattcgtcag catgtgctga acactacccg
     7081 actggtgaat aatctactgg atatggcgcg aattcagtcc ggcggcttta atttgaagaa
     7141 agagtggtta acgctggaag aagtagtcgg cagcgcgctg caaatgctgg aaccgggttt
     7201 atcgtcgccc atcaatcttt ctctgccaga accgctgacc ttaatccacg ttgacgggcc
     7261 actctttgaa cgggtgctga ttaatctgct ggagaacgcg gtgaaatatg cgggtgcgca
     7321 ggccgaaatt ggtatcgatg cccacgttga gggcgaaaat ctacaactgg atgtctggga
     7381 taacggcccc ggtcttccgc caggccagga gcagacgata tttgataagt ttgctcgcgg
     7441 gaataaagag tcggcggtac cgggggtagg gcttggactg gcaatttgtc gggcgatagt
     7501 ggatgtacac gggggcacta ttaccgcgtt caaccggccg gaaggtggtg cctgttttcg
     7561 tgttacactt ccccagcaaa ctgcccctga acttgaagaa tttcatgagg atatgtgaca
     7621 aacgttctga ttgttgaaga tgaacaggct attcgtcgct ttctgcgcac ggcgctggag
     7681 ggcgacggga tgcgcgtcta tgaggccgaa acgctgcaac gcggcttgct ggaagcggct
     7741 acccgtaagc cagatttgat tattctcgat ctcggcctgc ccgatggtga tgggattgag
     7801 tttatccgcg acctgcgcca gtggagcgcg gtgccggtga ttgtgctttc cgcacgcagc
     7861 gaagagagcg acaaaatcgc cgcgctggat gccggagcgg atgattatct aagtaagccg
     7921 tttggcattg gcgaattgca ggcccgtctg cgcgtcgctt tacgccgcca ctccgccacc
     7981 gccgcgcccg atccgctggt gaaattttcc gatgttaccg tcgatttagc cgcccgcgtg
     8041 attcaccggg gtgaggaaga ggtgcatctc accccgattg agtttcgcct gctggcagtg
     8101 ctgctcaaca acgccggaaa agtgctcacc cagcgccaac tccttaacca ggtgtggggg
     8161 ccaaacgcgg tcgaacacag tcattatttg cgtatttata tgggacatct gcgacaaaaa
     8221 ctggaacagg atcccgctcg ccccagccat ttca
//
LOCUS       DAZFAB010000155         8233 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZFAB010000155 DAZFAB010000000
VERSION     DAZFAB010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8233
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.155"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(230..305)
                     /locus_tag="ACHJWA_004338"
     tRNA            complement(230..305)
                     /locus_tag="ACHJWA_004338"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(270..272),aa:Asn,seq:gtt)
     gene            complement(406..1893)
                     /gene="yeeO"
                     /locus_tag="ACHJWA_004339"
     CDS             complement(406..1893)
                     /gene="yeeO"
                     /locus_tag="ACHJWA_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFO4310813.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     FAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSW
                     PGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIG
                     IPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITG
                     RRLGVGQIAQAEIQLRHVFWLTTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILI
                     WLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVG
                     VWMGMFADWAVRAVLFYWRMVTGRWLWKYPRPEPQKCEKKPAVSE"
     gene            2054..2129
                     /locus_tag="ACHJWA_004340"
     tRNA            2054..2129
                     /locus_tag="ACHJWA_004340"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2087..2089,aa:Asn,seq:gtt)
     gene            complement(2167..3117)
                     /gene="cbl"
                     /locus_tag="ACHJWA_004341"
     CDS             complement(2167..3117)
                     /gene="cbl"
                     /locus_tag="ACHJWA_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFO4310814.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLHNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPHDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIILSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEKNLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(3219..4136)
                     /gene="nac"
                     /locus_tag="ACHJWA_004342"
     CDS             complement(3219..4136)
                     /gene="nac"
                     /locus_tag="ACHJWA_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFO4310815.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            4462..4537
                     /locus_tag="ACHJWA_004343"
     tRNA            4462..4537
                     /locus_tag="ACHJWA_004343"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4495..4497,aa:Asn,seq:gtt)
     gene            complement(4593..5528)
                     /gene="ldtA"
                     /locus_tag="ACHJWA_004344"
     CDS             complement(4593..5528)
                     /gene="ldtA"
                     /locus_tag="ACHJWA_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO4310816.1"
                     /translation="MMRRVNILCSFALLFASHTSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPVTVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(5590..6669)
                     /gene="cobT"
                     /locus_tag="ACHJWA_004345"
     CDS             complement(5590..6669)
                     /gene="cobT"
                     /locus_tag="ACHJWA_004345"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFO4310817.1"
                     /translation="MQTLANLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALGV
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVNVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPFLNMDMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASKIVLPGNTTSDLNS"
     gene            complement(6681..7424)
                     /gene="cobS"
                     /locus_tag="ACHJWA_004346"
     CDS             complement(6681..7424)
                     /gene="cobS"
                     /locus_tag="ACHJWA_004346"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFO4310818.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLVLMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(7421..7966)
                     /gene="cobU"
                     /locus_tag="ACHJWA_004347"
     CDS             complement(7421..7966)
                     /gene="cobU"
                     /locus_tag="ACHJWA_004347"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFO4310819.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPEHWRTVERWQHLDELIHADINPNEVVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(8118..>8233)
                     /locus_tag="ACHJWA_004348"
     CDS             complement(8118..>8233)
                     /locus_tag="ACHJWA_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HFO4310820.1"
                     /translation="VPAVALIHYLNEMCQSTETAVNDAFCRTLTRESLAIP"
BASE COUNT         2277 a         2224 c         1780 g         1952 t
ORIGIN      
        1 aaggcatttt ttctttatct ttgtacggta cttcatggaa cagagttttt gaccttgcga
       61 atcgtgatgt ctgttggggg caatttactc actgaagcgt gagactcgat taagcgcacg
      121 aaacacagaa atcaaaaaac ccggtcactt tttacaaggc aaccgggtaa aaataatttt
      181 tattttttaa ctgccctgag actcataaag atgcctcaaa actaaaattt ggctcctctg
      241 actggactcg aaccagtgac atacggatta acagtccgcc gttctaccga ctgaactaca
      301 gaggaatcgt gagaacgagg cgaatattag cgatgcccac ccacaatgtc aaagcctgtt
      361 ttttaaattt gaaatcgttt gctgaaataa tctgcatttt gtcgtttatt ccgacacagc
      421 tggctttttt tcacactttt gcggttcggg tcgagggtat ttccacagcc aacgtccagt
      481 aaccattcgc cagtaaaaca gcacggcccg cacagcccag tcggcaaaca ttcccatcca
      541 gacaccaacc acaccccagc caagcatgat gcccagcaca taaccgacga caacccgaca
      601 accccacatg ctcaacatag aaacccacat agcgtaacgg gcgtcacgag cccccttaaa
      661 tccagcgggt agcacccatg aagcggacca aataggcata aataaagcat ttagccaaat
      721 cagaatcaca acgacatgtt taacctgtgg atcctgggtg taaaacgatg ccataacccc
      781 ggcaaaggga gccgttagcc aggcgatggc cgttaatcca agagtggtca gccaaaacac
      841 atgccgcaac tgaatctctg cttgcgctat ctgccctacc cccaaccttc ggcctgtaat
      901 gatcgtagaa gcagaaccga gcgcacttcc gggtaagttg ataagagccg caattgaaaa
      961 cgcgataaaa tttccggcaa taacactggt ccccattccg gcaacgaaca tttgggttaa
     1021 taaccgacca ctggtaaata acactgattc gacactcgct ggaataccaa tccccatgac
     1081 ttcccagata atgctaaaat tcagcggttt aaaatagctc tttaacgaaa tccttagcgc
     1141 aggattaaaa ccaatcgcca gcacccacaa aattgcaact gcgccaatat aacgagaaat
     1201 ggttaaaccc aacccagccc cgacaaatcc cagtcccggc caggagaaaa ggccgtaaat
     1261 caatatgccg ctaataataa tattaagaat attcaggcta ccgttaatca gtagcggtat
     1321 tttcgtattc cctgcaccac gaagtgcccc gctaccaatt agagtgatgg cagctgctgg
     1381 ataactgagt accgtcagct ccagataagt caacgccagt gctttaactt ctgtcgtggc
     1441 atcacccgcg acgaaatcaa taatttgctc gccaaaatga tgaataagcg ttgccaacag
     1501 tacggcaaac aacgtcatga tcaccaatga ctgccgcgtc gccaccctcg ctcgtcgtcg
     1561 atcccgctta ccgagactaa atgccacaac gacagtagta ccaagatcga tagcagcaaa
     1621 aaaagccata atgaccatat tgaagctgtc cgccaatccc acgccggcca tcgcatcttt
     1681 tcccagccag ctgaccagaa aagtgctcag aacccccatc aacaggacac aggcattctc
     1741 catgaagata ggaacagcaa acggggttat ctcgcgccag aacaacactt tgtagctctt
     1801 gcgtttagcg tgccagcgag tgccgtgaac aacctggcgt aaagcagagg agatattcaa
     1861 agccgacctt aattgcagaa agtgaaacca catttcaaat aatgagggag aatcagcaaa
     1921 gctgcaaaga ttttcgccat caaattgtct gcaaatgcaa caaactgttg atagaaacgg
     1981 caaacagttg ggaaaattaa aaatcgggtt tgacaaaaga tttttcgccg ttaagatgtg
     2041 cctcaacagc gattcctctg tagttcagtc ggtagaacgg cggactgtta atccgtatgt
     2101 cactggttcg agtccagtca gaggagccaa atttctgaaa agcccgcttt tatagcgggc
     2161 tttttgctat atctgataat caatttcctc ttcactgttt tccatcacct gccgcttgat
     2221 atcctcaact gaaagtcctg cattgcaaag ttccagaaag cgccagacat agttacgctg
     2281 aagttgtcct cgcttcagtc ccaaccagac agtattagca tcgaaaagat gccgcgtatc
     2341 caggcggatt aaattctttt cctcttgttc accgctggat tgttcggcaa ctaatccgat
     2401 cccaagccca agagcaacat aggttttaat gacatcagaa tcctgcgcac ttaatataat
     2461 atctgccagc aaacctttgc gggcaaatgc gtcatcaata cgtgagcgcc ccgtaatccc
     2521 ctgtcggtaa gtgattaacg gccacttcgc tattgattcc agcgtcaatg gtgtaatttg
     2581 cgtcaaggga tgatcgtgtg gaacaagcaa actatggtgc caacgaaacc acgggaaggc
     2641 gacgagctgc gggtcattac tcaaacgctc gctggcgata ccaatatcag cttcaccatt
     2701 atgcaacaac gtcgcaattt cctgtggcgt cccctggatt agctcgagcc gaacctccgg
     2761 gaaaagttcg cgaaaagctt taatgacctc tggcaagcta taacgtgcct gagtatgcgt
     2821 cgttgcaata gtgagaacgc cagacgtatc gttggtaaac aggtctgcaa gccgacgaac
     2881 attactggct tcattgagaa tacgttctgc aatgaccagt aatgctttgc ccggttcagt
     2941 catgcccagc agtcgcttac ctcgtcgaac aaatatttcg atgccaagtt catcctccag
     3001 ttcccgaata tgacggctga cgcctgactg tgaggtaaaa agcatattcg caacctctgt
     3061 caggttgtaa tcctgacgtg cagcttcgcg gattatcttt agttgttgga aattcacggt
     3121 aaactccggg cagttcagat atcccgttat tgttaaagtc taacgcccgg cataacaaat
     3181 aataaaaacc cgcatcttat tccatcccga tataacgctt agctcaccaa ttgccactgc
     3241 cttttttcca tcactggaga actaatcact gacattaaca actctttcac tgcctgtgcc
     3301 tgtggcgata agttcgctct ggcgggtaaa tttaatgaca aagagagact catggaaggc
     3361 gtggtaatgc gtgacatcca cccatttact gcgccacata acgaacgcgc ggccgattcg
     3421 ggtaatactg caacgcccat gccgctggca atcgctgcgg taagcgtagc aatagactca
     3481 atttcaccaa tgacttttgc cgtgagccgc cgtagggaaa acgcctcatc aacacgaagt
     3541 ctaacagcgc tgtaatcact ggggagaaaa aggttcattt gtgcaatagc attaacatca
     3601 acgctttgcc ccgggcaatc ttgagttcct accaggaaaa gatcttcttt cagcaaagcc
     3661 tgactggata caccagccac aggggaatgc tcataaatca ccgccatatc gagttggtga
     3721 tttatcagtt tttcgttaag cactgcaccg ctattttcat gaagatagat aacgacctcc
     3781 gggaattcag cgcgaaccgc ctgtaataag ggcatggtga tggatgacgc agcggttcct
     3841 ggcgcaaagc caatcgagac ttgccccgat aatgcctgac caacgttatg caccgccagt
     3901 tgggcctgct cacactgacg taaaatggcc cgcgcatggg tatagagaat ttttccggcg
     3961 tctgttggcg taacgccccg ttttgtgcga atcaaaagtt gttgatttaa ctcaccttcg
     4021 agtgtggcaa cctgctggct gagcgctggt tgtgcgatat gcaatacttc agcagcctga
     4081 gtcaggctac caatatctac aatttttacg aagtatttca ggcgtctgaa gttcatgttg
     4141 cctccggttt ttgagaatcg gcccaagtgc cgccattact tacaaccaga ttgcaagatg
     4201 cttgccagtt ttattttggc gttgatgtac aagctaacca actgtcaaat aagagattat
     4261 gatagattcg tcattggctc ttttcaccag cagtcgcgtt cccctgccct ataaaaggag
     4321 ggtatgcacc acgatggttc attaccaata agattgaaat ctcatcactt tgttgaaatt
     4381 gacagcaaac aaacaaaaaa atgcatttca ccctttgaca tcaccatgca ctgccattaa
     4441 tatgcgcccc gttcacacga ttcctctgta gttcagtcgg tagaacggcg gactgttaat
     4501 ccgtatgtca ctggttcgag tccagtcaga ggagccaaat tcaaaaaagc ctgctttcta
     4561 gcaggctttt tgctttctaa ttaccaacgc tcttaaaaca tctgtcttga accagaacta
     4621 atttgcacag gcattcccga tcgacgttgc aacgcagcat ttgcgcgatt cacatcaact
     4681 tcttgcccgt tgataaacgc ccgcaaagat ggggttaccg gcaatggcac ttttcggtca
     4741 gactcatatt cagcacgatt gcgcgacagc ggctcatgaa cttccagcca ctttgaacca
     4801 tccggttccg ttgtgtattt cactggctga tcaataattt gcacacgagt acctacagga
     4861 acattatcaa acagatattt gatatcgtca ttgcgcagac gaatacagcc ctgacttacc
     4921 cggagcccaa taccaaaatt ggcattggta ccgtggatgg catatagcct gccaatataa
     4981 atcgcgtaca gccccatggg attatcgggc cccgcaggaa caaatgcggg caaactctcc
     5041 cctcgtttcg catattcgcg ccgggtgttc ggcgttggcg tccaggttgg tgcttcttgt
     5101 ttacgttcaa cggtagtcac ccagttacgc ggggtttctc gcccagcctg gccgatacca
     5161 ataggaaaga cttccacagt attactgtct ggtgggtagt aataaagacg catctcagcg
     5221 acgttaacca caatcccttt acgaacagtg acgggcaaaa tcagttgctg cggaatggtg
     5281 agttgcgaac ctgacttcgg taagaagaca tcagcgcccg gattcgcttc cagcatgtta
     5341 cttaaccctt gcccgtattg tgcggcaaaa gtctccagcg gctgggtatt gtgatcagga
     5401 acagttacag taagcgactg ccccactaaa cggctaccct ctggaggtaa tggataagtt
     5461 accgccaggc tagtatggct ggcaaaaagc agagcaaatg agcaaagaat atttacacga
     5521 cgcatcatgt ccctttccta agtcgcgaaa gctatcggtt aagtatagct tttatcagac
     5581 ttttcgtttt taactgttca aatcagaagt cgtattcccc ggtagaacaa ttttactggc
     5641 agcaagttcg cccatgttgt tgtatatcgc acaagcagct tcgatgatgg gcatcgccag
     5701 agctgcgcca ctcccctcac ctaaacgcat atccatattg agaaaaggct ccagccccaa
     5761 atgcgacagc gctatacgcg cccctttttc tgccgacagg tgagaaggaa tgagatacgg
     5821 tttgattgca ggagacatct ggcaggctgc gagcgcagca gcataagaaa gaaatccatc
     5881 cagcaacacg ggtaaaccgc aggaagcagc gcctaacatc acgccagcca ttccgaccaa
     5941 atcaaatcca cccacttttg ccagaacatt aacgccatcc tgcggatttg gctgattcaa
     6001 cgtaatcgcc cggcgcacaa catcaatttt attagccagt ttatctgtcg gcaggtttgc
     6061 gccaatccca accacttctt caggatcccg gccagtgatt gtgctgacta ttgctgctgc
     6121 cggtgtcgtg tttgccatcc ccagttcacc tacaccaaac agcgtgacac cgttttttgc
     6181 cagctcccgc gtataacata tgacgtccaa aagtaacttt tcagcctgac ggcgactcat
     6241 tgccggagct gaagcaatat tgccgctacc tcgtgcgaca cgcatgttga taagcccggg
     6301 gataggctca gcagtatcaa taccaacatc aattacgtgg acgttagcgc ccgcttgtgc
     6361 tgccagcaca cacacgccgg ttgttccgcg ggtcatattt tcagcctgaa tggctgtcac
     6421 ttcttttggg gaaatagcga ccccttcctc ccagacgccg tgatcggcac acataaccag
     6481 taccgctttt ttgcccacat gcggtatgcc attcaacccc ggcattcccg ccagttgtac
     6541 gccaagcgcc tccagccttc ccaggctacc aacaggtttg agtaacccgt caatatgccg
     6601 ttgtgcacgc gacatagcgg caggatcgat ggcagggatc gtattcagta aattggcaag
     6661 tgtttgcatg tcacgtctct ttatagcagt gccagcagga agaccagttc accaagctct
     6721 atggctgctc ccagcgtatc gccggtttgc ccgcccagcg tacgtttgag aagctgaccg
     6781 aggatgaaaa tcgccaccat cgtcaccacc atagcagcca caccatgcat acccggcaat
     6841 aatacggcag caaaaatagc ggctaagccg agtgtgacgc aggtttgtcg cccatcaatt
     6901 ttgccaatga acacattgcc aagcccttct tcccgggcat aacgatgacg atacatcaat
     6961 aatgcggcag tcccgcggct gaccgcacat gctgccgcca gcgatgcaag gatcggttcg
     7021 ccacgcagag ccaactcgct taacaccaaa atctttgcca gtacaacaaa aatcaatgcc
     7081 agaccgccgt gggtacctaa acggctatca cgcatgattt ccaacatgcg atcgcggcta
     7141 cgtgcagaaa atacgccatc acaggtatca gcgaggccgt ccagatggaa tcccccggtc
     7201 atcagcacca gcacaagtac gctaaacagt gccgccagtg gtgcgccaca ccatgcctgc
     7261 agcaccatga agaccagccc gctaatcgcg ccaagcaata atccaatcaa aggaaaagta
     7321 ataataccgc gagaataatg ctcgaaatcc agtccctggg accagcgacg cggtacaggc
     7381 agacgcgtaa taaatgagag catcgcccaa aataatttac tcatttgatt tttactccaa
     7441 tacccgaaac caccagccat acttcatttg ccgcagcggc caactgctga tttacccgcc
     7501 cggcaatatc acgaaaatgt cgtgccagac gactctccgg cacaatcccc attcccactt
     7561 cgttagtcac taatacaacc tttgcggggc aacgttggca ggcagcaatc aacgactgaa
     7621 tctcagcatt aatcgcctgt tccatcgcct gataatccca ttcatcaggg tctttatcgc
     7681 cgccataatc aaacaacaga ttagtcacca ttgtggtaac gcattcaagc aacacaacct
     7741 cattcgggtt aatgtctgca tgaattaatt catcaagatg ttgccagcgc tccactgtgc
     7801 gccagtgctc cgggcggcct tgccgatgat gttctatccg tgcagccatc tcatcatcaa
     7861 ggatttgcga ggtagcgata tacagaacct gtgaagagtc cccaataagc gcctctgcgt
     7921 ggcgactctt cccgctccgt gcgccccccg tcaccaaaat catcataccg actcctgatt
     7981 caaatgtatg atggtattca ttgaacagaa caatggatcc cccaccgttg ctgaccgcaa
     8041 aacatttacg cgattaatac tgcgcgtaat ataattaaaa atccagcatt ctcaatccat
     8101 tacgaaataa gatggcatta cggaatagcc agactttctc gtgttaacgt ccgacaaaat
     8161 gcgtcattga cagcggtttc tgtggattga cacatttcat tcagataatg aattaatgct
     8221 actgccggaa caa
//
LOCUS       DAZFAB010000156         8185 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZFAB010000156 DAZFAB010000000
VERSION     DAZFAB010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8185
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.156"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(578..718)
                     /locus_tag="ACHJWA_004349"
     CDS             complement(578..718)
                     /locus_tag="ACHJWA_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO4310821.1"
                     /translation="MPQKTIIVGMLCLTMLLTVWVLHASPCEFRVSFMWSEIAAFLQC
                     KP"
     gene            complement(1020..1283)
                     /gene="ykgM"
                     /locus_tag="ACHJWA_004350"
     CDS             complement(1020..1283)
                     /gene="ykgM"
                     /locus_tag="ACHJWA_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFO4310822.1"
                     /translation="MKPNIHPEYRTVVFHDTSIDEYFRIGSTIKTDRVIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVDAKKGG"
     gene            1708..1833
                     /locus_tag="ACHJWA_004351"
                     /pseudo
     CDS             1708..1833
                     /locus_tag="ACHJWA_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
     gene            1898..2158
                     /locus_tag="ACHJWA_004352"
                     /pseudo
     CDS             1898..2158
                     /locus_tag="ACHJWA_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2208..2447
                     /locus_tag="ACHJWA_004353"
                     /pseudo
     CDS             2208..2447
                     /locus_tag="ACHJWA_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(2495..3112)
                     /gene="ureG"
                     /locus_tag="ACHJWA_004354"
     CDS             complement(2495..3112)
                     /gene="ureG"
                     /locus_tag="ACHJWA_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HFO4310823.1"
                     /translation="MNIIKQPLRVGVGGPVGSGKTALLEALCKSMRDTWQLAVVTNDI
                     YTREDQRILTEAGALEAERIVGVETGGCPHTAIREDASMNLAAVEALSEKFGNLELIF
                     VESGGDNLSATFSPELADLTIYVIDVAEGEKIPRKGGPGITKSDFLVINKTDLAPYVG
                     ASLEVMERDTLRMRGERPWGFTNLKSGEGLQNIIAFIEEQGMLGK"
     gene            complement(3124..3798)
                     /locus_tag="ACHJWA_004355"
     CDS             complement(3124..3798)
                     /locus_tag="ACHJWA_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HFO4310824.1"
                     /translation="MPTPEKRLRLMQLASNSLPVGGYSWSQGLEWAVEAGWVEDSAAF
                     EHWQQLQMEQSFFAVDLPLLARLYRACEAGDPDSAGRWTAYLLACRETRELRDEERNR
                     GAAFTRLLVDWQPDCPAEWRKLCQQSQLTGMAWLGVRWQISVSDLALSLGYSWIESAV
                     MAGVRLVPYGQLAAQQLIMRLCARYAANMDSALATPDHAIGSATPLASIASARHETQY
                     SRLFRS"
     gene            complement(3799..4263)
                     /gene="ureE"
                     /locus_tag="ACHJWA_004356"
     CDS             complement(3799..4263)
                     /gene="ureE"
                     /locus_tag="ACHJWA_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HFO4310825.1"
                     /translation="MLYLTRRVETPAQTTASVTLPVDMRVKSRIKVTLNDGRQAGLLL
                     PRGLLLRDGDILSNENGDEFIKVIAADEAVSVVRCADPFMLAKACWHLGNRHVPLQIM
                     PGELRYHHDHVLDDMLRQFGLDVDFAHLPFEPEAGAYASKSHAHNHDQEHSH"
     gene            complement(4273..5976)
                     /gene="ureC"
                     /locus_tag="ACHJWA_004357"
     CDS             complement(4273..5976)
                     /gene="ureC"
                     /locus_tag="ACHJWA_004357"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HFO4310826.1"
                     /translation="MSNISRQAYADMFGPTTGDKIRLADTELWIEVEDDLTTYGEEVK
                     FGGGKVIRDGMGQGQMLSAGCADLVLTNALIIDYWGIVKADIGVKDGRIFAIGKAGNP
                     DIQPNVTIPIGVSTEIIAAEGRIVTAGGVDTHIHWICPQQAEEALTSGITTMIGGGTG
                     PTAGSNATTCTPGPWYIYQMLQAADSLPVNIGLLGKGNCSNPDALREQVAAGVIGLKI
                     HEDWGATPAVINCALTVADEMDVQVALHSDTLNESGFVEDTLTAIGGRTIHTFHTEGA
                     GGGHAPDIITACAHPNILPSSTNPTLPYTVNTIDEHLDMLMVCHHLDPDIAEDVAFAE
                     SRIRQETIAAEDVLHDLGAFSLTSSDSQAMGRVGEVVLRTWQVAHRMKVQRGPLPEES
                     GDNDNVRVKRYIAKYTINPALTHGIAHEVGSIEVGKLADLVLWSPAFFGVKPATIVKG
                     GMIAMAPMGDINGSIPTPQPVHYRPMFAALGSARHRCRVTFLSQAAAANGVAEQLNLH
                     STTAVVKGCRTVQKADMRHNSLLPDITVDSQTYEVRINGELITSEPADILPMAQRYFL
                     F"
     gene            complement(5969..6289)
                     /locus_tag="ACHJWA_004358"
     CDS             complement(5969..6289)
                     /locus_tag="ACHJWA_004358"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HFO4310827.1"
                     /translation="MIPGEYKVKPGYIELNIGRATCSIIVENHGDRPIQVGSHYHFAE
                     VNPALKFDRQKARGYRLNIAAGTAVRFEPGQKREVELVALSGARIVHGFRGDIMGELE
                     ANDE"
     gene            complement(6298..6600)
                     /locus_tag="ACHJWA_004359"
     CDS             complement(6298..6600)
                     /locus_tag="ACHJWA_004359"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HFO4310828.1"
                     /translation="MELTPREKDKLLLFTAALLAERRLARGLKLNYPESVALISAFIM
                     EGARDGKSVAALMEEGRHVLSREQVMEGIPEMIPDIQVEATFPDGSKLVTVHNPII"
     gene            complement(6691..7389)
                     /locus_tag="ACHJWA_004360"
     CDS             complement(6691..7389)
                     /locus_tag="ACHJWA_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HFO4310829.1"
                     /translation="MLDLRFQRLHGKTTLTTRHHVGLLTVQRPFYPEEETCHLCLLHP
                     PGGIVGGDELIINAIIDSDCHTLITMPGASKFYRSSGAQAHLQQNLTLCNNATLEWLP
                     QDSIFFPGAHATLHTVFHLSSSSTLLAWDLLCLGRPVIGETFSHGTLANRLEIWVDGS
                     PLLIERLHVANGELTCVARKPWVGTMLFYLGNETQLEDIREKLTPLENYAGATLTDGL
                     LTVRFLSDDNLRCQ"
     gene            7770..8045
                     /locus_tag="ACHJWA_004361"
     CDS             7770..8045
                     /locus_tag="ACHJWA_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310830.1"
                     /translation="MTEHWSGHADDNFPFSWGSDSIFMHACLQSYGESICDTVHTFVA
                     DVAPESLTGLQTVYLYTAGLLCDIVFHIEGGPPEMNGIIIFPDDIIP"
BASE COUNT         1996 a         2061 c         2061 g         2067 t
ORIGIN      
        1 tatataatga tatgtatagt tttagcaata ccttctgcaa ttatcgtttt attctttcac
       61 ttttatcaaa acatcaccag aaactggaag tgacaaccgc aataatgaac ataaaaaggc
      121 aaatcagaat ggcagtaaat caataaagta ccatttcata tgaatcatca gaaaaacggt
      181 ggacgcgaca tccagcacga ggcaaaacac agcaacaaaa taagccggat atcgcgccct
      241 gtacttttct aactcaactc ccggcattgt gcaagtttta atcgcaggtg gattcagagc
      301 cacgccccca ttgattcagc ctgaagacac gtatatcctt ggagaaagca ggaatctcat
      361 tgctgtgaaa ttccttacag aatcttcagc cccattttat ggggcttttt ttggtgcccc
      421 cacgctgtta ccacactttc gggatgcgaa taaaaaagcc cacagttttt acactgcgga
      481 cttttgtttt ttctttgtga tttgtgtgca agggtgcgac tttcgaccga acctttggca
      541 accggatagc ggggactgtt gtccccgctg cggtttctta cggtttacac tgcaagaacg
      601 ccgcaatttc cgaccacatg aagcttaccc tgaattcaca cggtgatgcg tgtaacaccc
      661 agacggtcag caacatggtc aggcataaca tgcccacaat gatcgttttt tgcggcatag
      721 cacttgctcc ttttacagag aggcgctaac cttccacttg ctgaaggtag atggtcaggg
      781 cctcgggtta acgtttaagt tgactcgggg cctttccaca tccggccttc aggtaatcct
      841 tccagccgtc agccgaaagg cacccgcgcg taatgtactg gttttcagag aggtccgcaa
      901 tcttccggtg acgagatcat ctgcacacgc cccggtggga cgggctaacg gtatattcag
      961 gtgcaggatc tctctgtctg gtgcgttttt aaattgtcaa acgcaccctg gcggcaaaat
     1021 cagccacctt ttttcgcatc aacaaaacgc ccaaaacgct gggtaaagcg tgcaacatta
     1081 ccttctgaag cgacagttct cagtttacct gtataaaacg gatgcgactt agaagaaacg
     1141 tcaattgtaa catatgggta agttacacca tccagttcaa ttacccggtc agttttgatc
     1201 gtcgaaccga tcctgaagta ctcatcaata ctggtatcgt gaaatactac tgtacggtat
     1261 tccggatgga tattcggttt cacaacagtt ttcccccttt aagtgaaaat tccgcataaa
     1321 ggtacatatt cagacggtca gattcaactg tctgttctta taacctcact ctctttcagt
     1381 attacaatat gttatataat tataaactgg acttcccgaa aaaatccatt aaataagaag
     1441 cgagccgttg cggaggcttg ctctctcctt taacctcatg tgcaatatcc gtaacgcgct
     1501 cacataaatc atcatctgat aatataacaa cacccattat tttattctga cctaaaaact
     1561 tcttatgaaa taaacgttta cccgacgtat ctgatcccgc tgtttttacc tgtatccttt
     1621 aacatacttg agaagcccgc tgaaaaaata taccgtaaag gcggtttctc tgtcttccat
     1681 aaacctgtcg gataagaagg gcattaaatg aaaaccaaaa aagttatgat gattgcactg
     1741 gtcagctctg cgctggctct ttcaggatgt ggagccatgg gcacagcgat caaaaaacga
     1801 aatctggaag taaaaaccca gatgagtgaa acctgaggta gcctgagttt aacggacact
     1861 ccttcctgaa atagaatggc atcagaagga gctaataatg agcagaaaaa cccaacgtta
     1921 ctctaaagag ttcaaagccg aagctgtcag aacggttctt gaaaatcaac tttcgatcag
     1981 tgaaggcgct tcccgattat ctcttcctga aggcacttta ggacaatggg ttaccgccgc
     2041 cagaaaaggg ctcggtactc ctggttcccg cacggtggct gaactggaat ctgaaattct
     2101 gcaactgcgt aaggcgttaa atgaagctcg ccttgagcga gatatattaa aaaaagcagc
     2161 tgtagattca atctgtcaat gcaacacccc tttcaattat ctctttcact gcccgcgacg
     2221 gtatcggtta tcagtttgac ctgctggcta acggtgaaac cgtactgcgt gatgcatccg
     2281 gcgaaaaacg catcaagggt gaaaaagacg gtcgtatgct catgaatgtt ggcctgaatg
     2341 cggaaattcg cgacaacatc cgcttcggtc tggagtttga aaaatcggca tctggtaagt
     2401 acaacgtgga taacgcggtg aacgccaact tccgttattc cttctgataa aaacaagggg
     2461 ccgcctgccg gccctgatac agaggatgtt gtgattactt acccagcatg ccctgctctt
     2521 cgataaaggc gatgatgttt tgcagacctt caccactttt caggttagtg aacccccatg
     2581 ggcgttctcc gcgcatacgt aaagtatcac gctccattac ctcgagggag gcgcccacgt
     2641 atggtgcaag gtcagtttta ttgatcacga ggaagtcgga tttggtgatc cccggaccgc
     2701 ctttacgcgg gatcttttct ccttctgcca catctatgac atatatggta aggtccgcca
     2761 gttcggggct gaaggtcgca ctcaggttgt cgccgccgct ttccacaaag atcagctcga
     2821 gattaccaaa tttctcactc agtgcttcca cggctgccag attcattgat gcgtcttcac
     2881 gaatggctgt atgagggcag ccccccgttt cgacaccgac aatgcgttcc gcttccagtg
     2941 ctccggcctc agtgagaatg cgttgatctt ctctggtgta gatgtcgttg gtgactacgg
     3001 ctaattgcca ggtgtcacgc atggatttgc agagggcttc cagaagcgcg gttttacctg
     3061 agccgaccgg accgccgacg ccgacacgca gcggttgctt gattatgttc attttatttt
     3121 ctcctaagaa cggaataatc gggaatactg ggtttcatgt cgcgcggaag cgatagatgc
     3181 aagtggcgtt gctgaaccaa ttgcgtgatc cggggttgca agggcactgt ccatgttcgc
     3241 cgcatagcgg gcacacaggc gcataattaa ctgctgggca gccagctggc cgtagggtac
     3301 cagcctgacg cccgccatca ctgcgctttc aatccagctg tagccaaggc tcagggccag
     3361 gtctgaaacg gatatttgcc agcgtacgcc gagccatgcc ataccggtta actggctttg
     3421 ctggcatagt ttgcgccatt cggccgggca gtcgggctgc cagtcgacca gaagtctggt
     3481 gaaggctgca ccgcggttac gttcttcatc gcgtagctca cgggtttccc tgcaggccag
     3541 cagataagcg gtccagcgcc cggcactgtc aggatcgccc gcttcacagg cccggtatag
     3601 ccgggcaagc agtggcaggt cgacggcaaa aaaactctgt tccatctgca actgctgcca
     3661 gtgttcaaag gcggcgctat cttctaccca gcctgcttcc actgcccact ccagtccctg
     3721 ggaccagctg tagcctccaa cagggaggct gttactggca agctgcatca gccgcagacg
     3781 tttttccgga gtcggcattc agtggctgtg ctcctgatcg tgattatggg cgtgcgactt
     3841 gctggcgtaa gcgccggctt ccggctcaaa cggaagatgt gcaaaatcaa catccagccc
     3901 aaactggcgc agcatatcat cgaggacgtg atcgtgatgg tagcgcagct ctccgggcat
     3961 tatttgcagc ggaacgtggc gattgccgag atgccagcag gctttcgcga gcataaaagg
     4021 atctgcgcag cgcactacag aaacggcttc atcagcggcg atcaccttaa tgaattcatc
     4081 accgttttcg ttactgagaa tatcaccatc gcgcagcagc agaccgcgtg gcagcaacag
     4141 tccagcctga cgtccatcgt taagggtgac tttaatacgg ctttttaccc gcatgtcaac
     4201 agggagggta acgctggcag ttgtctgagc cggggtctcc acccgccggg tcaaataaag
     4261 catttgtgct ccttaaaaca ggaaataacg ttgcgccatt ggcagaatgt ccgccggttc
     4321 actggttatc agttcgccgt tgatacgcac ttcgtaggtt tgtgaatcca cggttatatc
     4381 aggcaacagg ctgttgtggc gcatatcggc tttttgtact gtgcggcagc cttttaccac
     4441 agcagttgtg ctgtgcaggt taagctgttc agcgacgcca tttgctgctg ctgcctgcga
     4501 caggaaagtc acacgacagc ggtgacgggc actgcccaat gcagcgaaca ttgggcgata
     4561 gtgcaccggc tgcggtgtgg ggatagagcc gttgatatca cccatcggcg ccatggctat
     4621 cattccgcct ttgacgatag tcgccggttt tacgccaaag aacgccgggg accagagcac
     4681 cagatccgcc agttttccca cttcaatcga gccgacttca tgagcaatac cgtgggttaa
     4741 tgccggatta atggtgtatt tagcgatata gcgcttcacg cggacgttgt cgttatcacc
     4801 actttcttcc ggtaacgggc cgcgctgaac tttcatccgg tgtgccacct gccaggttcg
     4861 taacactact tctccgacgc gtcccatggc ctgcgaatcg gacgaggtga gggagaacgc
     4921 gccaaggtcg tgcaggacgt cttccgcggc aatggtttcc tggcgaatgc gcgattcggc
     4981 aaaggctacg tcctcggcga tatccgggtc aagatgatgg caaaccatca gcatgtccag
     5041 atgctcatca atagtgttga cggtataggg tagcgtcgga ttggttgagg aaggcagaat
     5101 attggggtgc gcgcaggcgg tgataatatc cggagcatga ccaccacctg caccttctgt
     5161 atggaaggta tggatagtgc gcccgccgat ggcagtcaga gtatcctcaa cgaatcctga
     5221 ttcgttaagc gtatcactgt gtagcgcaac ctgaacgtcc atttcgtcgg ctacagtcag
     5281 tgcgcagttg attaccgcag gtgtagctcc ccagtcttcg tgaattttga ggccgataac
     5341 cccggccgcg acctgctcac gaagcgcatc cggattggag caattgcctt tacccagcaa
     5401 cccgatattg accggcaggc tgtctgcagc ctgcagcatt tgataaatgt accatggtcc
     5461 tggggtacag gttgtggcgt tagaacccgc tgtcgggcca gtaccgccac cgatcatggt
     5521 ggtaatgccg gatgtcagcg cttcttcagc ctgctgtggg cagatccagt gaatatgcgt
     5581 atcgacgcca cctgcggtaa cgatcctgcc ttctgcggca ataatttccg tggatacgcc
     5641 gattgggata gtgacgttgg gttgtatatc aggattaccg gctttgccga tagcaaatat
     5701 ccttccatct ttgacgccga tatcggcttt aacgatcccc cagtaatcga tgatcagggc
     5761 attggtcagc accagatcag cgcagccggc ggagagcatt tgcccctgtc ccataccgtc
     5821 gcggattact ttaccgccgc cgaatttgac ctcttcgccg taggtagtta aatcatcttc
     5881 gacctcgatc cacagctcag tgtctgccag acgaatttta tcaccggtgg tagggccgaa
     5941 catgtcagca taggcctggc gtgaaatatt actcatcatt tgcctccagc tcacccatta
     6001 tatctccgcg gaaaccgtgc acgatacggg caccgctcag cgccaccagt tcaacctcac
     6061 gcttttggcc tggttcgaag cgcactgcag ttccggcggc gatattgaga cgatagcctc
     6121 ttgccttctg gcgatcgaac ttcagtgccg ggttaacctc cgcgaagtga taatgggaac
     6181 cgacctgaat tggtcggtct ccgtgatttt caacgataat actgcaggta gcccggccga
     6241 tgttaagctc tatatagcca ggtttaacct tatattctcc agggatcatc tgttacctca
     6301 gattatcgga ttatggacgg taaccagctt ggagccgtcc ggaaaggtgg cttcgacctg
     6361 gatatcgggg atcatttctg gtatgccttc catgacctgc tcgcgactca ggacatgccg
     6421 tccttcttcc atcagcgcag cgacgctttt gccgtcgcga gcgccctcca ttataaaagc
     6481 gctaatcagg gccacggatt cgggatagtt aagtttcagg ccgcgggcca gacgacgctc
     6541 tgccagcagt gcagcggtaa acagtaatag tttgtctttt tctcttggag tcagttccat
     6601 agcgttctct taagtcagcc agatccgggg aagtaccggt gatttgtcag tcagttgcgg
     6661 ccgcataaat tgccagatat cacgcattac ctactggcaa cgcagattat catcacttaa
     6721 aaagcgaact gtcagtagac catcggtgag cgtggctccg gcgtagtttt ccagtggtgt
     6781 gagtttttcg cgtatgtctt ccagttgcgt ttcattcccc aggtagaaca gcatcgttcc
     6841 cacccatggt ttccgggcaa cgcaggtaag ttctccattg gccacgtgca gacgctcaat
     6901 caacagcgga gaaccgtcga cccatatctc cagacggtta gcgagtgttc cgtggctgaa
     6961 ggtttctccg atgaccggac gtccaaggca cagaagatcc caggccagca gtgtactgga
     7021 ggacgacaga tgaaacacgg tatgcaacgt ggcgtgagcg ccgggaaaaa aaatcgaatc
     7081 ctgtggcaac cactccagcg ttgcgttatt gcacagcgtc aggttttgct gaaggtgcgc
     7141 ctgcgcgccg ctgctgcggt aaaatttact ggcaccaggc atggtgatca gcgtatggca
     7201 atcagagtca attattgcgt tgatgatgag ttcgtcaccg ccgacgatac caccgggtgg
     7261 atgaagcaga cagaggtggc aggtttcttc ttcgggataa aaaggacgct gaacagtgag
     7321 cagaccgaca tgatgacggg tggtgagcgt ggttttcccg tgcagacgct gaaaacggag
     7381 atccagcatg gcctgccagc cttttttatt ttgtggtaac gcggtactga gcaatcttac
     7441 gcccctgata aataaagata aaacgacagt aaagagccgg atttccttgt tatataaaag
     7501 atgatgcctt tccagtgagt tttcgtctga ataatacgaa tgtttgtttt cgtcatgaag
     7561 cgttccggct cccggggcta tacatcgcca gaaaagcaat gcatattgtc gggattatcc
     7621 agagaatcag gcagaaagat ccgacaatgg gctgtgatta tgccatctgg ctcagaatga
     7681 tatcaaggtg ttatggggat aacttgctga tacaatagca gtatgacaga ttcattaatg
     7741 cggaaactgc tcactgaaac agagtgttca tgactgagca ctggtcgggt catgcagatg
     7801 ataatttccc cttttcttgg ggaagcgaca gtattttcat gcatgcctgt cttcagtcat
     7861 acggggagag catctgtgat acagtccata catttgttgc ggacgtcgct cctgagtcac
     7921 tgactggtct gcaaacagtt tatctgtaca cggcagggct tctgtgtgat atcgtttttc
     7981 atatcgaggg ggggcctcca gagatgaatg gaataataat atttcctgat gatataatac
     8041 cttagagaat ttctgtgtat gctattgtga acatattttt tattaaaaag atgttagtgg
     8101 tgttgtaaaa taaaagaatc agtaactata aacgccttta ttgacataaa gttttgatga
     8161 tgaagttgaa gtttgacctg gtata
//
LOCUS       DAZFAB010000157         8140 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZFAB010000157 DAZFAB010000000
VERSION     DAZFAB010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8140
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.157"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            91..1424
                     /gene="adeQ"
                     /locus_tag="ACHJWA_004362"
                     /gene_synonym="yicO"
                     /pseudo
     CDS             91..1424
                     /gene="adeQ"
                     /locus_tag="ACHJWA_004362"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
     gene            1477..1929
                     /gene="yicN"
                     /locus_tag="ACHJWA_004363"
     CDS             1477..1929
                     /gene="yicN"
                     /locus_tag="ACHJWA_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFO4310831.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2045..2368)
                     /locus_tag="ACHJWA_004364"
     CDS             complement(2045..2368)
                     /locus_tag="ACHJWA_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4310832.1"
                     /translation="MTAKTKFKSPAFEAIHSAAAGLSSVNAISAETMRTFDKACLTSV
                     QDLQPVEIKALREKLKVSQPVFAHYLNTSVSTVQKWESGAKRPNGISLKLLSIVQKHG
                     LEVLL"
     gene            complement(2352..2705)
                     /locus_tag="ACHJWA_004365"
     CDS             complement(2352..2705)
                     /locus_tag="ACHJWA_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO4310833.1"
                     /translation="MFKTKWFAREARSHAITDEELCRAILETEQGKADALGGGVFKKR
                     LHQNRERAIILAKGVSNWFYTFLYAKQDMSNINSQELAGFREIAKHYAFLTKAQLTAM
                     INTKELTEICYDCKN"
     gene            2939..4129
                     /gene="nepI"
                     /locus_tag="ACHJWA_004366"
     CDS             2939..4129
                     /gene="nepI"
                     /locus_tag="ACHJWA_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFO4310834.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGCFLGELIGWRNVFNAAAAMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMTLAGFGVDGLTLVLLSFGIASFVGTSLSSFIL
                     KRSVKLALAGAPFVLALSALVLTLWGSDKIVATGVAIIWGLTFALIPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(4170..4463)
                     /gene="yicS"
                     /locus_tag="ACHJWA_004367"
     CDS             complement(4170..4463)
                     /gene="yicS"
                     /locus_tag="ACHJWA_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFO4310835.1"
                     /translation="MKPTMLLMITVFLIFPALSQAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            4685..5503
                     /gene="nlpA"
                     /locus_tag="ACHJWA_004368"
     CDS             4685..5503
                     /gene="nlpA"
                     /locus_tag="ACHJWA_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFO4310836.1"
                     /translation="MKLTTHHLRAGAALLLAGVLLAGCDQSSSDEKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNNGELDANVFQHRPFLEQDNQTHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIEQTGLSPVNDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            complement(5507..6430)
                     /gene="yicL"
                     /locus_tag="ACHJWA_004369"
     CDS             complement(5507..6430)
                     /gene="yicL"
                     /locus_tag="ACHJWA_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HFO4310837.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLIGTFLLVTHGNPTSLSISPAALF
                     WGIASAFTAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYAGQGTNFIVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(6541..6714)
                     /locus_tag="ACHJWA_004370"
                     /pseudo
     CDS             complement(6541..6714)
                     /locus_tag="ACHJWA_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            6894..6999
                     /locus_tag="ACHJWA_004371"
                     /pseudo
     CDS             6894..6999
                     /locus_tag="ACHJWA_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
     gene            7010..7075
                     /locus_tag="ACHJWA_004372"
                     /pseudo
     CDS             7010..7075
                     /locus_tag="ACHJWA_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444509.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            complement(7450..>8140)
                     /locus_tag="ACHJWA_004373"
     CDS             complement(7450..>8140)
                     /locus_tag="ACHJWA_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFO4310838.1"
                     /translation="LPNTVNLSSLTYNKEDDFLYATQNSPAQLLKITKSGDIMDRAPL
                     PFISDAETIEHIQGNIFAAVDEKTSELFFFTVTKDMHISFRNKIQLEKFNKKNRGFEG
                     LAWKADDRMLFVAKERRPSKIFIYQLSPDLLSVKQATIPEALNDIRVNDISGLAFNNE
                     SLMILSDESRKLLKFNLTEMSFIEMLDLTKGNHSLTSDLPQPEGIVTLPDESIYVASE
                     PDILAKFVPNK"
BASE COUNT         2034 a         1759 c         1984 g         2363 t
ORIGIN      
        1 tgcaattgaa atgtgataat tatcactgaa ccgttgttgt catttgtttt acaaaagcaa
       61 gggattaaat ctcctccaat ggacaaaaaa atgaataatg acaataccga ttacgtgagt
      121 aatgaatcag ggacgctttc gcgattattt aaactacctc agcatgggac caccgtccgc
      181 acagaattga ttgcggggat gaccactttt ttaaccatgg tgtacatcgt ttttgtgaac
      241 ccgcaaatcc tcggcgcggc acaaatggac ccgaaagtgg tgtttgttac cacctgtttg
      301 attgccggta tcggcagtat tgcgatgggg atatttgcta acttacccgt ggcgctggct
      361 ccggcaatgg ggctgaacgc cttctttgcg ttcgtggtcg tgggggcgat gggtatctcc
      421 tggcagaccg ggatgggcgc gatattctgg ggcgcaattg ggctattttt gctcacgcta
      481 tttcgtatcc ggtactggat gatctccaac attccattaa gtttacgtat tggtatcacc
      541 agcggaatcg gattatttat cgccttaatg ggattaaaaa atactggcgt tattgtcgcc
      601 aataaagaca ctctggtgat gattggcgat ttaagttctc acggcgtgtt gttaggtatt
      661 ttagggtttt ttattataac cgtgttgtca tcacgtcatt ttcatgccgc ggtgcttgtt
      721 tctattgtgg tgacgtcttg ctgtggatta tttttcggtg atgttcattt tagcggcgtc
      781 tattccattc cgcctgatat tagcggcgtc attggtgaag tagatttgag cggcgcgtta
      841 acacttgaac tcgccggtat cattttctcc tttatgctga tcaacctatt tgattcatca
      901 ggaacattaa ttggtgtaac tgataaagct ggcttaatag atagtaacgg taaattcccc
      961 aatatgaata aggcgctata tgttgatagc gtagttcggt ggcgggcgcg tttatcggca
     1021 cctcgtctgt taccgcctat attgaaagta cttctggtgt ggcagtcggt ggtcgcacgg
     1081 ggctgactgc ggttgtggtc ggcgttatgt tcctgttggt tatgttcttc tcaccgctgg
     1141 tggcgatggt tcctccttac gcaaccgccg gagcgttaat ctttgttggc gtgttgatga
     1201 cctcaagtct ggcgcgcgtt aactgggatg attttaccga atcggtgcct gcgtttgtta
     1261 ccacggtgat gatgcccttt actttctcga tcaccgaagg gattgcactt ggctttatgt
     1321 cgtactgcat catgaaagtg tgcaccgggc gctggcgcga tctgaacctg tgtgtggtgg
     1381 tggtcgcagc tctgtttgca ctgaagatta ttctggtgga ttagtaactg gcgagggttt
     1441 ccagatatca tgagttctga ttacgcagga gaactcatga tctggataat gctcgccacg
     1501 ctggcggtag tgtttgtggt tggttttcgg gtgctgacat ccggggccag aaaagcgatt
     1561 cgccgtctca gcgatcggct gaatatcgat gtcgtacccg tggagtcgat ggtcgatcaa
     1621 atgggaaagt cagccggtga cgaattttta cgttatttgc atcgtccgga tgagtcgcac
     1681 ctgcaaaacg ccgcgcaggt gttgctcatc tggcaaattg tcattgtcga tggtagcgaa
     1741 cagaacctgc tgcaatggca tcggatttta caaaaagccc gccttgccgc tcccattact
     1801 gatgctcagg tcaggctggc gctgggtttt ctgcgcgaaa ccgaacctga aatgcaggat
     1861 attaatgctt ttcagatgcg ctataacgcg ttctttcagc ctgccgaggg cgttcactgg
     1921 ctgcattaaa accgcttcgt cttatcagcc tacagaccgc gcctgacctg tagggtggat
     1981 aaggcgttca cgccgcatcc ggtatcggaa ctgacagtag gtgaacgcat tatctacgtt
     2041 ctatttacag cagcacctcc aacccgtgtt tttgcacaat gctgagcagt tttaatgaaa
     2101 tgccattggg gcgtttagcg ccactttccc atttttgtac agttgaaacg ctggtattga
     2161 gatagtgagc gaagacgggc tgactcactt ttaatttttc ccgcagtgct tttatctcaa
     2221 caggttgtaa gtcttgcacg ctggtcaggc aggctttgtc aaaagtacgc atcgtttcag
     2281 cagaaatagc attgacgctg gacaatccag ccgctgcgct gtgaatagcc tcaaatgcag
     2341 gacttttaaa tttagttttt gcagtcatag cagatctctg tcagttcttt agtgttaatc
     2401 attgctgtta gttgggcttt cgtcaggaag gcataatgct ttgcaatttc acgaaaccca
     2461 gccagttcct gactattgat gttcgacata tcctgttttg catataaaaa cgtgtaaaac
     2521 caattgctta cccctttcgc cagaataatt gcacgctcac gattttggtg aaggcgcttt
     2581 ttaaaaacgc caccgccaag tgcatcagct ttaccttgtt ctgtttctaa aatggcccgg
     2641 cacaactctt catctgttat tgcatgagac ctggcttccc ttgcaaacca tttggtttta
     2701 aacacgcgta tatcgttgag gtcctgttat taaagtatat caccaagtgc aatactttat
     2761 cccttgttcc caacacaatc gcgaaaatac atatcacctt gtcctatgat ttttatttct
     2821 tcgagcagcc tcgagctttt cccacaacac cgtaaaagtt gcaaaaacgt taaatgcgtc
     2881 acacatttca atgttaaagt ggtcggcttt tcccctgaaa catgccacgg gtaacaccat
     2941 gagtgaattt attgccgaaa accgcggcgc ggatgccatc acccgaccga actggtcagc
     3001 cgttttctcg gtggcgtttt gcgtcgcctg tctgattatc gttgagtttt tgcctgtcag
     3061 tttgttgacg ccaatggccc aggatttagg catttcggaa ggggttgccg ggcaatcggt
     3121 gaccgtgacc gcctttgtgg caatgtttgc cagtttgttt attacccaga caattcaggc
     3181 cactgaccgc cgctacgttg ttattttgtt tgccgttttg ctgacgctct cctgcttgct
     3241 ggtttccttt gctaactcat tcagtttgct tttaatcggt cgtgcctgtc tggggctggc
     3301 gctgggcggg ttctgggcga tgtcggcgtc gctgaccatg cgtctggtgc cgccgcgtac
     3361 ggtgccgaag gcgctgtcgg tgatcttcgg cgcggtttct attgcgctgg tgattgccgc
     3421 gccgttgggc tgttttttag gcgagcttat cggttggcgc aatgtcttta atgcggcggc
     3481 ggcgatgggt gtgctgtgta ttttctggat tatcaaatca ctgccttcgc tgccaggtga
     3541 accttcgcat cagaaacaaa atactttccg cttattacaa cgtccgggag tgatggccgg
     3601 gatgatcgcc atctttatgt cctttgccgg acagtttgct tttttcactt atattcgccc
     3661 agtgtatatg actctggcgg gattcggtgt tgatggctta accctggtgc tgctgagttt
     3721 tggtatcgcc agctttgtcg ggacatcgct ttcgtcgttt attctcaaac gttcggtgaa
     3781 actggcctta gcgggcgcac cgtttgtgct cgccctgagc gcgctggtac tgacgctgtg
     3841 gggaagcgat aaaattgttg ctactggtgt ggcgattatc tgggggctga cctttgcttt
     3901 aattcccgtt ggttggtcaa cgtggatcac tcgctcattg gccgatcagg cagaaaaggc
     3961 tggatctatt caggttgccg ttattcagct tgccaatacc tgcggtgcgg caatcggcgg
     4021 ttatgcgttg gataacattg gtctgacttc gccgctgatg ttgtccggca cattgatgtt
     4081 gctgactgca ttgttggtga ctgcaaaggt gaaaatgaag aaatcctgat atcaggtaaa
     4141 gaagcgttgg tcagtggttg ttgccgttct tacatatccg ggcattctac cttacccaac
     4201 gcttcccaat aattgctctg attattacgt tccatcgcca cgatggcctc gcgtatatgt
     4261 tgtttattgt tttcatctga caacattaac ttttcccacg cttcatctga taatgacgcc
     4321 tgtggcgtgc aaattttacg taaatcttgc aataccgtgg ttttctcttt tgccgactgt
     4381 agagaactga atggcgactc ggcctgagaa agggctggaa aaatcaggaa tacggtaatc
     4441 atgagtagca tcgttggctt catagcggca accttctgca aatgtgcatc gaatgaagca
     4501 taagtgtagt ttgcttttgc cgctctcatc atgctgtacg aaaggtgttt gcgtggtcag
     4561 taagaagtgc cagactttat attccacttt tattcctttt tattcttatc gatagcgttt
     4621 tgttttttaa actgcagcga ccttaccgct atagtccggt aatcattaat aaaaggataa
     4681 aaaaatgaaa ctgacaacac atcatctacg ggcgggggcc gcattattac tggcaggagt
     4741 tctgctggca ggttgcgacc agagcagcag cgatgaaaag catattaaag ttggcgttat
     4801 taatggcgca gaacaagatg tcgcagaagt cgctaaaaag gtagcaaaag agaaatatgg
     4861 cctcgatgtt gaactggtag gatttagcgg ttcgttgcta ccgaacgatg cgacaaataa
     4921 tggcgagctt gatgcgaacg tcttccagca tcgacccttt cttgaacaag ataatcagac
     4981 gcatggttat aaactggtag cggtgggaaa tacgtttgtc ttccctatgg cgggctattc
     5041 caaaaaaatc aaaaccgtgg cgcaaataaa agaaggtgcg acagtggcga tccccaacga
     5101 cccgactaac cttggtcgcg cgcttttact gctgcaaaaa gagaagctaa ttactctgaa
     5161 agagggaaaa gggttattac ctaccgcgtt ggatataacc gataatccac gccatttgca
     5221 gataatggag ctggaagggg cacagttgcc acgtgtactg gacgacccga aagtggatgt
     5281 agcgattatc agcaccactt acatcgagca aacaggactt tctccggtta acgacagcgt
     5341 atttattgaa gataaaaatt cgccgtatgt gaatattctg gtggcacggg aagataataa
     5401 gaatgcagaa aacgtgaagg aatttctgca atcttatcaa tcacccgaag tcgctaaagc
     5461 ggcagaaacc atctttaacg gtggtgcggt gcctggctgg taattctcac tcatgccgcg
     5521 ccggacgatt tatttttctg gcgcggcggc gggaatccat tgaaatcaaa atcaccgatg
     5581 acagaatcag cagcgttccc agccagtccg gcaatgtgaa ggtgatcccc agcaacagca
     5641 aagagagcag cgcgctactt aatggttcag cacagcttaa aatgctggct tttggaccgc
     5701 caattaattg tgctcctttc aggtacagac taaatgtcag ggacgtacca atgaccacca
     5761 aataaaaaaa cgccagaatc aaacttccgt taacgataaa gtttgtccct tgtccggcat
     5821 aaaagggcag caggatcaga ccgccaatga gcatactcca gccgacgacc ggtaacgtgc
     5881 catagcgggc aatcagcgtt gagggatagg tggtatagaa tgcagcagta aatgccgagg
     5941 caatgcccca gaataacgcg gcaggagaga tcgataatga cgtcggatta ccgtgtgtca
     6001 ccaataaaaa agtcccgatc agcgatgtca aaatagcgca gaaaaccaga atgcccgggc
     6061 gggatttacg caccagtgag aaccaggcga cgataatagt cggtgagagg aattgcagca
     6121 ccgttgccgt ggctgcgttc gatttttcga tggttagcaa aaaagtgagc tgtacggtta
     6181 gcgcgccaac cacggaaaaa atcagtaggc taatggcatc tttgtgattg ttaataatag
     6241 aaaagatttt atcaccatga acaaatgata gcgtcagcag aatcaaaccg gcgaatatca
     6301 aacgcgtcat agtcaaaaac tgcgacgaca tctggctttg ctccatgatg tattgcgcgc
     6361 agacccctga acttccccac aacacggcgg caatcagaac gttcagcatc ccctttctgg
     6421 tggaacccat ttttccctct gcatactgtt ttgtcgttta tgtatgcagg catgatagca
     6481 aaaatggcga ggatggctca gtttgcgcca cgccgatttt tcttagccca gactggtgag
     6541 ctaaatatct ttaataaaca gcaagcaaat cattgcaata cccaacatcc ctatcgccag
     6601 ccagaacaac gtgtggtagc tccagatttc aaccataatt tcgtcaacag agccggcgat
     6661 aatctagccg aagcgtgagg tatttgcata taacgttgtt gcggaaccta ttcttattta
     6721 tcggagtgcc ttgccagtaa tttactgacg aggatcctca taatagcgtt acccatctgt
     6781 tgcattgaga ctttacaggc cgttattcta aatgaccaaa attaaatcag tgcataaggg
     6841 gaagtattct gtagtgatgt ctccctgaga tgcaggttcc actggagaaa attatgagca
     6901 gtttgtttca gaatgaaata aaggataaat ttaaaactgg acttgcatat gtgcgggggc
     6961 cagaaaaaag cggatcttcc gctcaaaatg caggtagcga aggtggtaaa aaatgcgccg
     7021 ggagaagcag aagattgtcc cgcgatgtcc gtttgagaaa cacgagtttg attaaacgcg
     7081 tgagcatgat gatattagta gctgaccaga gaaattatat ccgtcgtaag caataaaaat
     7141 cagaagtgat gttatggata tttttaacct gaataactcc gtaagtacgg ggttattcag
     7201 gtttttgcta gcctgtgctt ttctatacca gatgtggtgt gaacactcta ttctgcatac
     7261 aaattaaata tattgtagga tctaatataa gtctgataat actaatagac tgagtaagat
     7321 tctggttgat aataactgat atccttttca actattaatg tgtttattta gcgtgaaatt
     7381 atactcgtat ttttataact ttatgagatt gccaggtttg gtcgataaat aataaggcca
     7441 gtgacagact tacttattag ggacaaattt cgcgaggata tcaggttcac tggcaacata
     7501 aatgctttca tcaggcagag ttacaattcc ttcaggttgg ggcaaatcac tggtcagaga
     7561 atgatttcct tttgtcaggt caagcatctc aatgaaagac atctctgtca ggttaaactt
     7621 gagtaattta cgcgattcat cggacagaat catcagactt tcattattaa aggccaggcc
     7681 ggatatatcg ttaacacgta tatcatttag tgcctctgga atagttgctt gctttacact
     7741 cagcaggtct ggagaaagtt gatatataaa tattttactt gggcgtcttt cttttgcgac
     7801 aaacagcatc cgatcatctg ctttccatgc cagcccttca aaccctctat tctttttatt
     7861 gaatttttct aactgaattt tatttcgaaa ggagatatgc atatcttttg taactgtaaa
     7921 aaagaatagt tcagaagtct tttcgtctac tgcagcaaaa atgttccctt gtatgtgttc
     7981 aattgtttcg gcatcagaga taaaaggcag tggggctctg tccatgatgt cgccactttt
     8041 ggttatttta agtagctgtg cagggctatt ctgggtggca tacaggaaat catcctcttt
     8101 attataagta agagatgata agttcaccgt atttggtaat
//
LOCUS       DAZFAB010000158         8060 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZFAB010000158 DAZFAB010000000
VERSION     DAZFAB010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.158"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(192..677)
                     /gene="sixA"
                     /locus_tag="ACHJWA_004374"
     CDS             complement(192..677)
                     /gene="sixA"
                     /locus_tag="ACHJWA_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFO4310839.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGKGEFNWQMSPCNLKMAKA
                     I"
     gene            complement(880..3024)
                     /gene="fadJ"
                     /locus_tag="ACHJWA_004375"
     CDS             complement(880..3024)
                     /gene="fadJ"
                     /locus_tag="ACHJWA_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFO4310840.1"
                     /translation="MEMTSAFTLNVRLDNIAIITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTEVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGIP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVAHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3024..4334)
                     /gene="fadI"
                     /locus_tag="ACHJWA_004376"
     CDS             complement(3024..4334)
                     /gene="fadI"
                     /locus_tag="ACHJWA_004376"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFO4310841.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVVESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQLLAEDNNIRGNSSLADFAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     ADLTLIDMHEAFAAQTLANIQLLGSERFAREALGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4514..4798)
                     /locus_tag="ACHJWA_004377"
     CDS             complement(4514..4798)
                     /locus_tag="ACHJWA_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFO4310842.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5170..6510
                     /gene="fadL"
                     /locus_tag="ACHJWA_004378"
     CDS             5170..6510
                     /gene="fadL"
                     /locus_tag="ACHJWA_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFO4310843.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGKTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRVFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            6875..7906
                     /locus_tag="ACHJWA_004379"
     CDS             6875..7906
                     /locus_tag="ACHJWA_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310844.1"
                     /translation="MLSSISINNTSAAYPESINENNNDEVNGLVQEFKSLFNGKEGIS
                     TCIKHLLELIKNAIRGNDDPDRININNSSVTYINIGSNDTDHITIGIDNQEPIKLPAS
                     YKDKELVRTIINDNIVEKTHDDINNAEFTDGGIDDRSDIGIDSNDTDDIDIDNQEPIK
                     SPANDENKKVGQTIINENIVENTHDINNKKMIFSAIKEIYDEDPSFIFDKISHKLRHT
                     VTEFDESGKSEPTGLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDGYHIQKGFR
                     IDGDNLTQYCSKKARSASAWFEHSKAIIAESFATGSDHQVVNELKGERLRELNEAFNP
                     SNLARGYEY"
BASE COUNT         2198 a         2041 c         1827 g         1994 t
ORIGIN      
        1 ttgatgcttt gtaaaaatac attacaactt agtctcattt cgtcagaaat taatagggaa
       61 gcagaggaaa aagtgcgatg aattctgtat cgaagcgtat caactccgtg aagggttttg
      121 agtgaatttc tactattgca aaaagacaaa tgcccgatgt acagcttaca tcgggcattt
      181 tttcaaacga atcagatagc tttcgccatc ttcaaattac atgggctcat ctgccagttg
      241 aactcgcctt ttccgctttc atcaagcgtt acgctggcaa tggccgaggt ggtaaacata
      301 ggcggcgttt cgcccggaca taactcggcg accagatacc caactaacgg taagtgggag
      361 ataaccaaca ccgaagcaac accttcattg gtcaacgcct gcaaataggc actgaccaga
      421 ccaacatcgc cgcagggcgt taactccggc agaacttctg cactggaagg caggttcaga
      481 caatccccaa cttcctccag tgtttgctcg gctcgcagga acgggctcac cagaacgcgt
      541 tcgatttcca ctttttgacc tttcagccag ttcgccatca ggcgagattc gtcacaaccg
      601 ttagtggtca gaggacgaac cgaatcactg gcggcatcga gggctgcgtc gccgtgacgc
      661 atgataaaaa cttgcatatt gcaccgcttt tgttaaccag attcgcccgt tttctttcat
      721 ctgaaaccag agaaagaaaa cggtggccgg cattgtgcct tatccattca ccgaatgaaa
      781 cgctgttttt tacctcaatg gcgtaagtat agtcaatatg cgtttacatt ttgaccaaca
      841 atccgccatt cagcgcggat tcatatagct ttgaccttct tattgcaggt cagttgcagt
      901 tgttttccaa aaactttccc cacgcgcacc catctcgact aaacgctcgc aaggggtaaa
      961 acgtgaacca tactgcgtgg ccagtcgttg cattattgca accacttcgc ccgcgccgag
     1021 agaatcgata tagcgaaacg gtccaccgag aaatggcgga aaaccaatgc caaataccgc
     1081 accaatatcc ccgtcacgca cgctacggat aacctgctca tcaacacaac gtacagcttc
     1141 attcagcatc aacatcacac accgttcagc aacctgcggt gcggagagtc gcccctgccc
     1201 ttgtgcgcca atgagcgggt aaatggcagg atcgacctgt tttttgcttt tacgcccttt
     1261 ctgaccataa agatagaaac ctcgaccatt ttttctgcct ttgcgatcgt cgttcaaaat
     1321 tgaagaaaca acatttgcag gcgcgctaaa acgttctcca taagcggctt ccagtacagg
     1381 aataatttta gtcccggtgt cgattcctac ctcatccaaa agttggattg gacctaccgg
     1441 aaaaccaaat ttcactagcg cggcatcaat gtgcgctacc cgttcacctt cggtcagcat
     1501 gcggatagct tcattaatgt aaggcgctaa gatgcgattg acgtaaaacc cggctttgtc
     1561 acgcacgaca atgggcgttt tgccctgttt tttcgccagt tttaccgtag tggcgatggt
     1621 ttgtgctgat gtacccgcat gaggaataat ctccaccagc ggcatttttt ccaccggact
     1681 gaagaaatgc aggccgataa cttgctcagg tcgcgtggcg tgagcggcga tatcaccaat
     1741 cggtaaagat gacgtattcg aagcaaagat ggtatgagcg gcgcaatttt gctcaacttc
     1801 cgccaccatc tgttgtttca attcgagatt ttcaaacacc gcttcaataa tcagatcgcg
     1861 atgggcaaag ccgcgatagt ccgtcgttcc ggagattaat gccagctgtt tgtcacgttc
     1921 gctggctttg agatgacgac ggcgaacttt gccctccagc tgatcccaac tgtacttcag
     1981 cgcatgattt atgccctgcg ggttgatatc tttaattctg accggaatcc ccgctttaca
     2041 agcagtgaca taagcaatac cgccgcccat caagccacca cctaaaatcc ccacgctgtt
     2101 taatggcgca ggcggcgcat cactgccggg atctttcttc acttccgtac tggcaaaaaa
     2161 gatactacgc aaagcttgcg attgtggcgt catcgccagt tcgccaaacg cccgtgcttc
     2221 agcgtcatag ccactgctgg tgccctgcgc taatcccgtt tcaacaacct ccaggatgcg
     2281 ttctgtcgcc ggataattgc cttgagtttt gtgttctgtt ttcttgccga ccattttgaa
     2341 cagcagcgca cgacctaacg gccccgccag aatacgctcg cgtacaggta gagggcggga
     2401 agatgggcga tccttctttg ccagctcaac agcggcttcc agcagaatgg agtgcggaac
     2461 aacatcatcc accagcccca gctttaatgc ctgtttcgcc cgaagttgtt ttccggtgag
     2521 gatcatctct aatgctgtgc tgacgcctat cagacgcggt aaacgctggg tgccgcctga
     2581 accgggtaac aatccaagtt gtacttcagg caaaccgagc accgttttag gatcgtcagt
     2641 acaaacgcga ccgtggcacg ccagcgccaa ctccagccca ccacccaggc aagcgccatg
     2701 aatagccgcg ataaccggaa tgggcaaagc atgaatctcc gccatcaact gttggccctg
     2761 ccgcgccaga gcttccgctt cttgcgccgt tttgcaattg ccgatcatgt tgatgtctgc
     2821 gccagcaatg aagttgtccg gtttagcgga gacaaacacc acgcctcgca actctttgtt
     2881 ttcacggagt tgcttaataa tggcgcgcac ctgcgaggca aactccgcct tcagggtatt
     2941 cattttctca cccggtacgt cgatggtgat gatagcaatg ttgtccagac gaacattaag
     3001 agtaaacgct gatgtcattt ccattattcc gcctccagaa ccattgccgc gccaagccca
     3061 ccggcagcac aggcggtaac taaaccaaat ccaccgccgc gacggcgaag ttcatgcaat
     3121 gtctgggtaa tcatccgcgc gccggtcgcc gcgaagggat gcccgtaagc aatcgaaccg
     3181 ccaagcacgt taaatttgct atcgtccact tcgccagtgg catgtgcacg ccccagcgct
     3241 tcacgagcaa aacgttcact acccagtagc tgaatattgg ccaacgtctg ggcggcaaag
     3301 gcttcgtgca tatcaatcaa tgtcagatct gccattgtca aaccggcacg ctccagcgcc
     3361 agcggtgttg accaggctgg accgagcaac atgtcctgcc agacatcgat agcggtaaat
     3421 gcgtagctgc gcagatatcc cagcggtacc agccctaatt ctttcgcccg ggattcagtc
     3481 atcaggatca ccgccgctgc gccatcggtc agcggcgtac tgttcgccgc cgttaccgtt
     3541 ccgtgtttac gatcaaacgc cggacgcagc tttgcgaaat cagcaagcga ggaattaccg
     3601 cgaatattgt tgtcttccgc gagcagttgt ttataaggag ggataaaggc agtcatcacc
     3661 tcttctttga gttttccgtc tgaccatgcc tgagcggcac gctgatgcga acggtgcgct
     3721 aatgcatctt gctgttctcg ggtgatgccg taggttttcg ccatttgctc tgcagtgtcg
     3781 cccatccgca agccggtaga atattctgct accgcaggtg gtacgggcat taagtcgcgc
     3841 aaacgcaggc gagagaagag tttcagacgc tggctcattg tgcgggcttt gttgacatca
     3901 accagcacgc gcgccagttt tttactgacg ccaattggca ataccgaaga ggaatctgcc
     3961 ccaccggcaa tccccgctcg gatagtcccc gccatcaggc tttctacgac gtttgcaacc
     4021 gcctggaaac tggtagcgca agcgcggctg acgctgtaag catcggtatg cacattcatt
     4081 ccagtaccga gaacaatttc acgcgcaatg ttgggagctt caggcatttg tacgacctga
     4141 ccaaagacca gttgttcaat cacttcggcg gggatctcgc tgcgtgccag cagttcgcct
     4201 accaccatct tccctaaatc aaccgcggga atgccatgaa aagccgtcgc ctggcgggca
     4261 aaaggcgtac gtaaaccgct aacaatggcg atacgatcgc cctggcgggt aaccagcggt
     4321 aaaacctgcc ccataacact cccctgtaaa aaaataaata aagtggtctg acctgatcat
     4381 agtcttaacc atttttttac atttagccaa gcggagaaaa gggaaattgg gagctatgac
     4441 acagtgaaga caggagaaag aaaagaaaac gcccctacct gttgctggca ggagcgtact
     4501 tatccgagag gaattaacgc agacccagct ggaagatcag catttcagct tcgcaggcaa
     4561 aagtgaaatc gatatccagg cgcacaccat cagactcttc agtgaaagtc ggggagattt
     4621 tgcacggttc ggattccacg ctacgggctt tctcagtcag cgccgccagc gtttgttctg
     4681 cttctgcgcg gtttgcaaac acgcggctgt aagacgctgt gcagtcggag ttgtccataa
     4741 tggtgccaac atccatacag cagcaaaccg gggtttcatc agcactacat ttactcatcg
     4801 ttgatttcct ctgtatgtgc acccaaggtg ccagataaac gttgtggata ttttacgctt
     4861 ccggaaagtg ctgctccagt tgttaattct gcaaaatcgg ataagtgacc gaaatcacac
     4921 ttaaaaatga tctaaaacaa aattcacccg aatccatgag tgcgccacct ccaaattttg
     4981 ccagctggat cgcgtttctt agatcatatt tgaaaaaaga tagaaacata cttgcaacat
     5041 tccagctggt ccgacctata ctctcgccac tggtctgatt tctaagatgt acctcagacc
     5101 ctacacttcg cgctcctgtt acagcacgta acatagtttg tataaaaata aatcattgag
     5161 gttatggtca tgagccagaa aaccctgttt acaaagtctg ctctcgcagt cgcagtggca
     5221 cttatctcca cccaggcctg gtcggcaggc tttcagttaa acgaattttc ttcctctggc
     5281 ctgggccggg cttattcagg ggaaggcgca attgccgatg atgcaggtaa cgtcagccgt
     5341 aaccccgcat tgattaccat gtttgaccgc ccgacatttt ctgcgggtgc ggtttatatt
     5401 gacccggatg taaatatcag cggaacgtct ccatctggtc gtagcctgaa agccgataac
     5461 atcgcgccta cggcatgggt tccgaacatg cactttgttg caccgattaa cgaccaattt
     5521 ggttggggcg cttctattac ctctaactat ggcctggcaa cagagtttaa cgatacttat
     5581 gcaggcggct ctgtcggggg tacaaccgac cttgaaacca tgaacctgaa cttaagcggt
     5641 gcgtatcgct taaataatgc atggagcttt ggtcttggtt tcaacgccgt ctacgctcgc
     5701 gcgaaaattg aacgtttcgc aggcgatctg gggcagctgg ttgctggtca gattatgcaa
     5761 tctcctgccg ggaagactcc tcaagggcaa gcattggcag ctaccgccaa cggtatcgac
     5821 agtaatacca aaatcgctca tctgaacggc aaccagtggg gctttggatg gaacgccggt
     5881 atcctgtatg aactggataa aaataaccgc tatgcactga cctaccgttc tgaagtgaaa
     5941 attgacttca aaggtaacta cagcagcgat cttaatcgtg tgtttaataa ctacggtttg
     6001 ccaattccta ccgccacagg tggcgcaacg caatcgggtt atctgacgct gaacctgcct
     6061 gaaatgtggg aagtgtcggg ttataaccgt gttgatccgc agtgggcgat tcactatagc
     6121 ctggcttaca ccagctggag tcagttccag cagctgaaag cgacctcaac cagtggcgac
     6181 acgctgttcc agaaacatga aggctttaaa gatgcttacc gcatcgcgtt gggtaccact
     6241 tattactacg atgataactg gaccttccgt accggtatcg cctttgatga cagcccagtt
     6301 ccggcacaga atcgttctat ctccattccg gaccaggacc gtttctggct gagtgcaggt
     6361 acgacttacg cgtttaataa agatgcttca gtcgacgttg gtgtttctta tatgcacggt
     6421 cagagcgtga aaattaacga aggcccatac cagttcgagt ctgaaggtaa agcctggctg
     6481 ttcggtacta actttaacta cgcgttctga taacgcgttc gcctggataa agtcacctgc
     6541 tatgcaggtg actttaactc ccccacttca ccgaaagtag tttcctcatt gcttgcctcc
     6601 tgagttttgc aaacagcctg atggcagcta gccctttcaa atcagtaagt ggtatccaca
     6661 gacaaacctg aagggaaacg catattttca ggcgttctgc tcgtccttct caaagagttt
     6721 acttttctga atttccagga tactcaaccc cctggctatt gtgcgctcat acacgcaaat
     6781 taaagatagg ttctaaataa atgagcgttt tttgatagtc tatttcatta ggtaatatat
     6841 atttgtaaca aatcaatcaa catggaataa aatcatgcta tcatctattt caatcaacaa
     6901 taccagcgca gcttacccag aatccatcaa tgaaaataac aatgatgaag ttaatggatt
     6961 agtacaagag ttcaaaagcc tttttaatgg taaggaagga ataagcacct gtattaaaca
     7021 tctacttgag cttataaaaa acgccatacg aggaaacgac gatcctgata gaataaatat
     7081 caataattct tcagttactt atattaatat tggttccaat gatacagacc atattactat
     7141 tggtatcgac aaccaagaac caataaaatt acctgcgagt tataaagaca aagaactcgt
     7201 ccgtactatc attaatgaca acatagttga gaagactcat gatgatataa ataatgctga
     7261 atttactgat ggtggtattg atgatcgtag tgatattggc attgactcca atgatacaga
     7321 cgatattgat attgataatc aagaaccaat aaaatcaccc gcgaacgatg aaaacaaaaa
     7381 agtcggccaa accatcatca atgaaaacat agtagagaat actcatgata tcaataacaa
     7441 gaaaatgatc ttcagcgcaa taaaagaaat atatgatgaa gatccaagtt ttatcttcga
     7501 taaaatatca cacaaactca gacatacggt aacggaattc gatgagagcg ggaaaagcga
     7561 accaacgggc ttatttacct ggtacggtaa agataaaaaa ggcgactctc tcgctattgt
     7621 aattaaaaat aaaaatggaa atgattactt atctctcggt tactacgatc aggatggcta
     7681 ccacattcaa aaaggatttc gtattgatgg tgataatctc acccaatatt gtagtaaaaa
     7741 agccaggagt gcttctgcgt ggtttgaaca cagtaaagca atcattgcag aatcatttgc
     7801 aacaggttcc gatcatcaag ttgtaaacga actcaaaggg gaaagactga gagaacttaa
     7861 cgaagctttt aatccttcaa atctagcaag aggatatgaa tattaagagg atgatagaaa
     7921 atatacattt gagaatacaa aagaaatagt ttctacttta gtcggtaaca aagttaacgt
     7981 tggccattct caaaaaatat ataaacattt taaggatctc gaaggtaaaa ctgaagaaag
     8041 gcttcaaaat cgccaggctg
//
LOCUS       DAZFAB010000159         8021 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZFAB010000159 DAZFAB010000000
VERSION     DAZFAB010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8021
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.159"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..386)
                     /gene="gpU"
                     /locus_tag="ACHJWA_004380"
     CDS             complement(<1..386)
                     /gene="gpU"
                     /locus_tag="ACHJWA_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail terminator protein"
                     /protein_id="HFO4310845.1"
                     /translation="MKHREIRAAVLSALKENISERVSWFDGRPVFIDEQELPAVAVYL
                     TDASAADEFVDEGTWEATLHIEVFLRAKEPDSALDMWMEEKILPALEAVPGLSALLLK
                     MNLQGYDYRRDDEFMMWGSADLLWKIT"
     gene            complement(399..1022)
                     /locus_tag="ACHJWA_004381"
     CDS             complement(399..1022)
                     /locus_tag="ACHJWA_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4310846.1"
                     /translation="MAIKGLAQAMKNLDAIDRRAVPRASAMTLNRVAGAIIAKTASSV
                     ARELAVPRRLIRARIRLSPARPDKVYAKVYINTGNLPAIKLGEARVRLSRRKRRKKGQ
                     RAALKGGGSVLIVGKRRIPDAFITRLANGRWHVMQRMPWASSSTGADSKGRPKRHRLP
                     IEVVKITTAGPLAETFERERDRMYREKLPAQMMKAMTHQLRLVLKRK"
     gene            complement(1025..1306)
                     /locus_tag="ACHJWA_004382"
     CDS             complement(1025..1306)
                     /locus_tag="ACHJWA_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HFO4310847.1"
                     /translation="MSDLFTRMCCRMDVATVRVMGKQAEINGVVYDVMPEEESAEMGA
                     LSGSQLSLVVFSARYRPARHDVVVFAGRTLTVTRYDTYNGKPRIFVEQE"
     gene            complement(1299..1625)
                     /locus_tag="ACHJWA_004383"
     CDS             complement(1299..1625)
                     /locus_tag="ACHJWA_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid cement protein"
                     /protein_id="HFO4310848.1"
                     /translation="MAKNFVQDGTTIELVNAGDQTILSGAAVVVGSMVAVAITDIPAG
                     EAGDGFAEGVFLLPKQSADDIQSGAVVYLKDGVVQLAADGAVAAGVAWENAPANSATV
                     AVKINV"
     gene            complement(1713..3737)
                     /locus_tag="ACHJWA_004384"
     CDS             complement(1713..3737)
                     /locus_tag="ACHJWA_004384"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HFO4310849.1"
                     /translation="MPELNDSISATPKASMKNKSWFRMQAGGPGDADIYIYDEIGFWG
                     VTAKQFVSELNALGDITHINLHINSPGGDVFEGIAIFNALKNQGATITVYVDGVAASM
                     ASVIAMAGDTVIMPENAFMMIHKPWGFSGGDAEDMRSYADLLDKVESVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAHGFADQVTPAVEAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRDTTVTIPAPAVTEPSPVPAVSDEATIRARVMAEQKARMSGINDLFAMF
                     GGRYQTLQAQCVADPDCSLEMARERLLNEMGKESSPTNKNTPAHIYAGNGNFVGDGIR
                     QAMLARAGFENVEKDNAYNGMTLREWARMSLTERGIGVASYNPMQMVGLALTHSTSDF
                     GNILLDVSNKGLIQGWEESEETFQKWTRKGRLSDFKTAYRVGMGGFGSLRQVREGAEY
                     KYITTSDRKETIALATYGEIFSITRQAIINDDLNMLVDVPMKMGRAAKATIGDLVYKV
                     LTDNPKLSDGKALFHADHKNIATGGISVSGLDAARQMMRLQKEGDRALNIRPAFMLVP
                     VALETVANQTIKSASVKGADANAGVINPIQNFAEVIAEARLDAADPKTWYLAAAQGTD
                     TIEVAWLDGVDTPYIDQQEGFTTDGIATKIRIDAGVAPLDWRGLVRSSVA"
     gene            complement(3682..5184)
                     /locus_tag="ACHJWA_004385"
     CDS             complement(3682..5184)
                     /locus_tag="ACHJWA_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4310850.1"
                     /translation="MAIIDDVIGVFSPGWKAARLRSRALIMAYEAVKPTRTHKARREN
                     RSADQLSKYGAVSLREQARFLDINHDLVIGVFDKLEERVIGARGIIVEPQPLRKNVEM
                     AAELAADIRRLWAEWSVSPDVTGQYTRPVLERLLLRTWLRDGEVFAQMVSGAGNGLER
                     TAGVPFWLEAMEPDFVPMRTDESAGLNQGVFLDEWGRPKKYLVYKNYPVRGRQSDTKE
                     IAAGKMIHLKFTRRLHQTRGSSMLSGVLMRISALKEYEDAELTAARIAAALGLYIRKG
                     DGQDYEDPGSKETEREVHITPGIIYDDLRKGEDIGMVKSDRPNPNLETFRNGQLRAVA
                     AGSRLSFSSAARNYNGTYSAQRQELVESTDGYLILQDCFIGAVTRPVYRTWLNMVVAA
                     GLLKIPADVEMKTLYNATYSGPVMPWIDPVKEAEAWRIQIRGGAATESDWVRAGGRNP
                     DEVKRRRKAEIDENSRLGLVFDTDPVNDKGGNSAGTERQYQRDTESQHEE"
     gene            complement(5184..5396)
                     /locus_tag="ACHJWA_004386"
     CDS             complement(5184..5396)
                     /locus_tag="ACHJWA_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310851.1"
                     /translation="MSTEEAREMIQRYREAEMAVLEGKSVTFNGQQLTLESLSQIRAG
                     RQEWERRLAAMVSRRRGKPGFKLARF"
     gene            complement(5393..7516)
                     /locus_tag="ACHJWA_004387"
     CDS             complement(5393..7516)
                     /locus_tag="ACHJWA_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO4310852.1"
                     /translation="MNQVNESHSRASDIWREVASLFRPPSRLPVAEAIRRYMRVPRGA
                     NTSGPWESSLTPYMIDPINTLSAREYDAVVFVGPARTGKTEGLIDGWIVYGIICDPAD
                     MLVVQMTETKAREHSRTRLSRTFRHSPEVSKRLSPSRNDNNVHDKMFLDGSFLKIGWP
                     SITVFSSSDYRRVALTDYDRFPENVDGEGDAFTLASKRTTTFMSSGMTLVESSPGRDI
                     TDTKWRCGGAHEAPPTTGILSLYNRGDRRRWYWPCPHCGEYFQPVMDNMTGYRNNPDF
                     VAAGQAARLMCPHCRGLIAPEQKRELNNQGIWLREGERAAADGSITGTPRNSRIASFW
                     MEGPAAAFQTWEQLIFKLLAAEEEYERTGSEETLKAVVNTDIGRPYLPRSATEQRKSE
                     LLEQRAEPFPRRSVPDGVRFIEATVDVQGGKNRRFVVQITGYGEQGERWIVDRYNIRH
                     SLRCSPNGESLPVDPAAYPEDWDLLLTDVFHKTWPLASDPDVRMRLMAMAVDTGGEAG
                     VTDNAYRFWRRCRSDGLGNRVFLFKGDGLRRDRLINRTFPDNTGRSARRARASGDVAL
                     WLVQTDAFKDRVNNALWRDTPGPNYIHFPDWLGRWFYDELTYEERGSDGKWRKPGRGA
                     NEAFDLLVYADALAVLHGYEKIRWPSAPDWAQRETWLVFPQERSSETVSPELTAGAEK
                     RRRRKKKLWTERAEDNPWITSGGWL"
     gene            complement(7513..7989)
                     /locus_tag="ACHJWA_004388"
     CDS             complement(7513..7989)
                     /locus_tag="ACHJWA_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFO4310853.1"
                     /translation="MDRELKNLHLNISQLAALSGAHRQTVAARVKNISPAGGHESNLK
                     LYRLTDILAELMKAPLPVDNEEMDPHARKAWYQSERDRLKFEQETGQLVPVSDVRRSF
                     SVVVKAIVQVLETWPDRLERDRGWTASQLNEVQIVVDEIRDTLEKAVIDCCDEADM"
BASE COUNT         1721 a         2523 c         1917 g         1860 t
ORIGIN      
        1 gtaattttcc acaggagatc tgccgatccc cacatcataa actcatcatc ccggcggtag
       61 tcatacccct gaagattcat cttcagcagt aatgcactga gcccgggaac tgcctccagc
      121 gcaggaagaa ttttttcttc catccacata tccagtgccg agtccggttc ttttgccctg
      181 agaaaaacct caatatgcag tgtcgcctcc caggttccct catcaacgaa ctcgtcagca
      241 gcagacgcat ctgtcaggta aacagcaaca gcaggcagtt cctgttcatc aataaaaacc
      301 gggcgaccgt caaaccagct caccctctca gaaatatttt ctttcagggc agacagaact
      361 gccgcccgta tttcacggtg tttcatacac cctcccagtc attttctttt cagcaccagg
      421 cgtaactgat gcgtcatggc tttcatcatc tgcgccggta atttttcccg gtacatccgg
      481 tcccgttcac gttcaaaggt ttctgccagc ggtccggcag tcgtaatctt caccacttcg
      541 atcggcagac ggtggcgttt cggcctccct ttgctgtccg cgccggtgga tgatgatgcc
      601 cacggcatac gctgcatcac atgccagcgt ccgttagcca gccgggtgat aaaggcgtcc
      661 gggatccgtc ttttccccac aatcagcaca ctgccgcccc ctttcagggc cgcacgctgt
      721 cctttctttc tccgttttct gcgggaaagt cgaacgcggg cctcccccag tttgatggcg
      781 ggcaggttgc cggtattgat gtaaaccttt gcgtaaacct tatccggtcg tgccggactt
      841 aaccggatgc gggcacggat aagacgacgg ggaacggcca gctccctggc aactgaagag
      901 gccgttttcg caataatggc ccccgccact cggttcagtg tcatggcaga ggcccgggga
      961 acggcacggc gatcaattgc atccagattt ttcatggcct gcgccagacc ttttattgcc
     1021 atactcattc ctgttcgaca aaaatccggg gtttaccgtt gtacgtgtca taacgggtca
     1081 ccgtcagtgt gcgccccgca aacacaacaa catcatgacg ggccggacgg taccgggctg
     1141 aaaacaccac cagtgacaac tggctgcccg aaagcgcccc catctccgcg gactcttcct
     1201 ccggcatcac gtcgtacacg acgccgttaa tctccgcctg tttgcccatc acccgaacgg
     1261 tcgccacgtc catccggcaa cacattcgcg taaacagatc agacattgat ttttaccgcc
     1321 acagtggcgc tgtttgcagg ggcattttcc caggctaccc ccgctgccac cgcaccgtct
     1381 gcagccagct gcacaacccc gtccttcaga taaaccaccg cgccggactg aatgtcgtca
     1441 gcagactgtt tgggcagcag gaacacgcct tcggcaaaac cgtcaccggc ctcaccggca
     1501 ggaatatcgg taatggccac ggccaccata ctgccgacca ccaccgcagc accgctcagg
     1561 atggtctgat ctccggcatt caccagttca atggtggtac cgtcctgtac aaaatttttc
     1621 gccataatgc tgtttctccg gacagcccct gtggggctgt ttttcaggca taaaaaaagc
     1681 cctttcgggc agtgattgtg ataacgcggt tatcaggcca ccgacgaacg caccagcccg
     1741 cgccagtcaa gtggtgccac tccggcatca atacggattt ttgtggcaat gccgtcagtg
     1801 gtgaaacctt cctgctgatc aatgtatggc gtgtccacac catccagcca ggccacttca
     1861 atggtgtcag tgccctgtgc cgccgccaga taccaggttt tcgggtctgc cgcatcaaga
     1921 cgcgcttctg caatcacctc agcaaagttc tggatagggt taatgacacc ggcgtttgca
     1981 tccgcccctt tcacactggc cgatttgatg gtctggttcg ccaccgtctc cagtgccacc
     2041 ggtaccagca taaaggccgg acggatattc agggcacgat cgccttcttt ctgcaggcgc
     2101 atcatctgac gggccgcatc cagtccggaa acggagatcc ccccggtggc aatatttttg
     2161 tgatcggcat ggaacagcgc cttaccgtcg gacagtttcg ggttatccgt cagcaccttg
     2221 tagaccaggt caccaatcgt tgccttcgcc gcacgcccca tcttcatcgg cacgtccacc
     2281 agcatattca gatcatcatt gataatggcc tggcgggtga tggagaaaat ctccccgtaa
     2341 gtggccagtg caatggtctc cttgcgatct gaggtggtga tgtatttata ctccgccccc
     2401 tcacgaacct ggcgcagaga accaaaaccg cccatcccca cgcgatacgc tgttttgaag
     2461 tctgacaggc gtcccttacg ggtccacttc tggaaggttt cttctgattc ctcccagccc
     2521 tggatcagcc ccttgttcga cacatccagc agaatattgc caaaatcaga ggtgctgtgc
     2581 gtcagcgcca gcccgaccat ctgcatgggg ttataactgg ccaccccaat accgcgctcc
     2641 gtcagtgaca tgcgagccca ttcacgcagg gtcatcccgt tataggcgtt atccttctcg
     2701 acattttcaa atccggcacg ggccagcatc gcctggcgga tcccgtcccc cacaaaattg
     2761 ccgtttccgg cataaatatg ggccggtgta tttttgttgg tcggcgagga ctccttgccc
     2821 atttcattca gcagtcgttc acgggccatt tccagcgaac agtcaggatc agccacgcac
     2881 tgtgcctgaa gcgtctgata gcgaccaccg aacatggcaa acagatcgtt aatgcctgac
     2941 atgcgggctt tctgttctgc cataacgcgg gcgcgaatgg tcgcctcatc agacactgcc
     3001 ggtaccggtg atggttctgt taccgccggt gcagggattg tcactgtggt atcacgcggg
     3061 gcactgttgc gtggcggagt aatcatgttt cggatggatt ccggcatctt tttaaattcc
     3121 tctgtacgtt ttgactgaat acatgccatt gcctcaacag cgggtgtcac ctggtcagca
     3181 aatccgtgtg ccagacattc ggcaccggac atccaggttt catccgccag catggcggca
     3241 atttcatcgg tggtttttcc ggttttctgc gcataggctg gcaacagtac cgattcgact
     3301 ttatccagca aatcggcata actgcgcata tcctcagcat ccccgccact gaatccccat
     3361 ggcttatgga tcatcatgaa ggcattttcc ggcataatga ccgtatcacc ggccatcgca
     3421 atcacagatg ccatcgaggc ggcaacgcca tccacataca cggtaatggt cgccccctga
     3481 tttttcaggg cattaaaaat ggcgatgcct tcaaagacat cgccacccgg tgaattgata
     3541 tggagattaa tgtgggtgat atcacccagt gcattcagtt cgctgacaaa ctgcttcgcg
     3601 gtaactcccc agaaaccaat ctcgtcataa atataaatat ccgcgtcacc cggcccccca
     3661 gcctgcatcc tgaaccagga tttattcttc atgctggctt tcggtgtcgc gctgatactg
     3721 tcgttcagtt ccggcactgt tgcctccttt gtcgttgacg gggtcagtat caaagaccag
     3781 ccccagtctg ctgttttcat caatttcagc cttgcggcga cgtttgacct catccggatt
     3841 gcgcccacca gcacgcaccc agtcagattc tgtcgctgca ccaccccgga tctgaattct
     3901 ccaggcttca gcttccttaa ccgggtcgat ccacggcatc accggaccgg aatacgtcgc
     3961 gttatatagc gttttcatct ccacatccgc cggaattttc agcagacctg ccgcaaccac
     4021 catattcagc catgtccggt acaccgggcg ggttaccgcg ccaataaaac agtcctgcag
     4081 gatcaggtaa ccatccgtgg actcgaccaa ctcctgccgc tgggcgctgt aggtgccgtt
     4141 atagttacgc gccgcactgg aaaaactcag acgactgcct gctgccactg cacgcaactg
     4201 gccgttgcgg aaagtttcaa ggttgggatt gggacggtca gatttgacca tgccgatatc
     4261 ctcgcccttg cgcaaatcgt cataaataat acccggggtg atatggactt cccgctcggt
     4321 ctctttgctc cccggatctt catagtcctg tccgtcacct ttacggatat acagtcccag
     4381 cgccgcagca atacgcgccg ctgtcagttc cgcatcctca tactccttaa gggcactgat
     4441 ccgcatcagc acccccgata acatggatga gcctcgcgtc tgatgcagac gacgagtgaa
     4501 cttcaggtgg atcatttttc cggcagcgat ttctttcgta tcactctgcc ggcctctgac
     4561 cggataattt ttataaacca gatatttttt cggtcttccc cactcatcaa gaaaaacccc
     4621 ctgattcagt ccggcggatt catcagtgcg catgggaaca aaatccggct ccatcgcctc
     4681 aagccagaat ggcactcccg ccgtccgttc cagaccgttt cccgcaccac tgaccatctg
     4741 cgcaaacact tcaccatccc gcagccaggt ccgcagcagt aaacgttcaa gcacaggacg
     4801 ggtatactgc cctgtcacat ccggactcac ggaccattca gcccacaaac ggcggatatc
     4861 cgcagccagc tcagccgcca tttccacgtt ttttcgtaat ggctgaggct ccacaataat
     4921 tcccctggca ccaatcaccc gctcttccag cttgtcaaac acaccaatca ccaggtcatg
     4981 attgatatcc agaaaacggg cctgctcccg cagggaaacc gcaccgtatt tactgagctg
     5041 atcagcagag cgattttccc gccgggcttt atgtgtccgg gtcggtttca ccgcctcata
     5101 ggccatgatt aacgcccttg aacgcagtct ggctgctttc cacccggggg aaaacacgcc
     5161 gatcacatca tcaataattg ccattaaaac ctcgccagtt taaatcccgg ttttccccgc
     5221 ctgcggctca ccatcgcggc aagcctgcgt tcccactcct gacgtccggc gcggatctga
     5281 gaaaggcttt ccagcgtcag ttgctgcccg ttgaaggtga cagactttcc ctccagtacg
     5341 gccatttccg cttcacggta ccgctgtatc atttctctgg cttcttctgt gctcacaacc
     5401 agcctcctga tgttatccat ggattatctt ccgcacgctc cgtccacagt tttttcttcc
     5461 ggcgacggcg tttttctgcc ccggccgtca gttccgggga taccgtttca ctagaacgct
     5521 cctgcgggaa gacgagccac gtttcccgct gtgcccagtc cggtgcggag ggccagcgga
     5581 tcttttcgta accatgcaga acggcaagcg catccgcata aaccagcagg tcaaacgctt
     5641 cgttagcgcc cctgcccggt tttcgccatt ttccgtcact gccgcgctct tcataggtca
     5701 gctcatcgta aaaccaccgc cccagccagt cgggaaagtg gatatagttc ggccctggtg
     5761 tgtcacgcca cagggcatta tttacacgat ccttaaacgc atccgtctga accagccaca
     5821 gcgcgacatc gccactggct ctggcacggc gggcacttct gccggtatta tccgggaagg
     5881 ttcggttaat cagcctgtca cggcgaagtc catccccctt gaacagaaac accctgttgc
     5941 ccagtccgtc actccggcaa cgacgccaga aacgataggc gttatctgtc accccggctt
     6001 cccctcccgt atccaccgcc atggccatca gacgcatgcg cacatccgga tcagaagcca
     6061 gcggccatgt tttatggaac acatccgtca gcaacaaatc ccagtcctcc ggatatgccg
     6121 ccggatcaac cggcagactt tcaccgttgg gactgcagcg cagtgaatgc cggatgttgt
     6181 agcgatcaac aatccagcgt tccccctgct ctccgtatcc ggtgatctgc acaacaaaac
     6241 ggcgattttt accgccctgt acgtcaaccg ttgcctcaat aaaacgcaca ccatccggca
     6301 cagatcgccg gggaaacggc tcggcacgct gttcaagcag ttcactttta cgctgttccg
     6361 tggctgaacg gggcagatag ggtcgtccga tatcggtgtt caccaccgct ttcagggtct
     6421 cttcactgcc ggttcgctca tactcttctt ctgccgccag cagtttaaaa atcagttgtt
     6481 cccaggtctg aaacgccgca gctggcccct ccatccaaaa tgacgcaatc cgggagtttc
     6541 gtggcgttcc ggtgatactg ccgtccgccg ccgcccgttc accttcacga agccagatcc
     6601 cctggttatt cagttcgcgt ttctgctcag gggcgatcag cccgcgacaa tgcggacaca
     6661 tcagacgggc agcctgaccg gcagccacaa aatccgggtt attccggtat ccggtcatgt
     6721 tatccatcac cggctgaaaa tattccccgc agtgcggaca cggccagtac caccggcggc
     6781 ggtctccccg gttatacagt gacaggatcc ccgttgttgg cggtgcctca tgtgcgccac
     6841 cacaacgcca tttggtatcg gtgatatccc gccccggtga actctcgacc agggtcatcc
     6901 ccgaggacat aaaggtggtg gtacgctttg aggccagcgt gaaggcatcc ccttccccgt
     6961 ccacgttttc agggaaacgg tcataatccg tcagcgccac acgacggtaa tccgaagagg
     7021 aaaagacggt gatcgacggc cagccaatct tcaggaagga gccgtcaaga aacattttat
     7081 cgtggacgtt gttgtcatta cgggaaggac tgaggcgctt gctgacctcc ggactgtggc
     7141 gaaacgtcct ggaaagacgc gttctggaat gctcacgcgc cttcgtctca gtcatctgca
     7201 ccaccagcat atccgccgga tcacagatga tgccgtacac aatccagcca tcaatcagcc
     7261 cttcggtttt cccggttcgc gcaggtccca caaacaccac cgcgtcatat tcacgggctg
     7321 ataatgtatt aatggggtca atcatatagg gcgtcagcga tgactcccac ggaccggaag
     7381 tattggctcc ccgtggaacc cgcatataac gcctgatggc ttccgctact ggtaaccggc
     7441 tgggtgggcg aaacagcgag gccacttcgc gccagatatc ggatgcgcgg ctatggctct
     7501 cgttcacctg attcacatat cggcctcatc acaacagtca atgactgcct tttccagtgt
     7561 gtcgcggatc tcatcaacca caatctgtac ttcattcagt tgtgatgcgg tccaccccct
     7621 gtccctctcc agccggtcag gccaggtttc cagtacctga actatcgctt tcaccacgac
     7681 agaaaaggac cgcctgacat cactgactgg cacaagctga ccagtttcct gctcaaattt
     7741 cagtcggtca cgttctgact ggtaccatgc tttacgcgca tgaggatcca tttcctcgtt
     7801 atctacaggc agaggagctt tcatcagctc ggcaaggata tctgtcagtc ggtacagttt
     7861 gagattgctc tcatgaccac cggctgggct tatgtttttt acccgagccg caacagtctg
     7921 tcgatgagca ccggataatg cggccagttg ggaaatattc agatgcagat tttttaattc
     7981 acgatccata actccccctg aaaattatgt aaacacacac c
//
LOCUS       DAZFAB010000160         7993 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZFAB010000160 DAZFAB010000000
VERSION     DAZFAB010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7993
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.160"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            96..635
                     /gene="elfA"
                     /locus_tag="ACHJWA_004389"
     CDS             96..635
                     /gene="elfA"
                     /locus_tag="ACHJWA_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4310854.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGSVTFNGKVIAPACT
                     LVAATKDSVVTLPDVSATKLQTNGQVSGVQTDVPIELKDCDTTVTKNATFTFNGTADT
                     TQITAFANQASSDAATNVALQMYMNDGTTAIKPDTETGNILLQDGDQTLTFKVDYIAT
                     GKATSGNVNAVTNFHINYY"
     gene            718..1419
                     /gene="elfD"
                     /locus_tag="ACHJWA_004390"
     CDS             718..1419
                     /gene="elfD"
                     /locus_tag="ACHJWA_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO4310855.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSTWAAGKGGVGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKLVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            1444..4043
                     /gene="elfC"
                     /locus_tag="ACHJWA_004391"
                     /pseudo
     CDS             1444..4043
                     /gene="elfC"
                     /locus_tag="ACHJWA_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            4034..5104
                     /gene="elfG"
                     /locus_tag="ACHJWA_004392"
     CDS             4034..5104
                     /gene="elfG"
                     /locus_tag="ACHJWA_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4310856.1"
                     /translation="MQIIFGEKCVSLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTTGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            5132..5659
                     /gene="ycbU"
                     /locus_tag="ACHJWA_004393"
     CDS             5132..5659
                     /gene="ycbU"
                     /locus_tag="ACHJWA_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4310857.1"
                     /translation="MFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDVATKSA
                     LQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAPTLLKL
                     TDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKSTKAGAT
                     GGNATAVLYFDLVYQ"
     gene            5667..6182
                     /gene="ycbV"
                     /locus_tag="ACHJWA_004394"
     CDS             5667..6182
                     /gene="ycbV"
                     /locus_tag="ACHJWA_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4310858.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFSHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            6148..6884
                     /gene="ycbF"
                     /locus_tag="ACHJWA_004395"
                     /pseudo
     CDS             6148..6884
                     /gene="ycbF"
                     /locus_tag="ACHJWA_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
     gene            6995..>7993
                     /gene="pyrD"
                     /locus_tag="ACHJWA_004396"
     CDS             6995..>7993
                     /gene="pyrD"
                     /locus_tag="ACHJWA_004396"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFO4310859.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIV"
BASE COUNT         2170 a         1748 c         2020 g         2055 t
ORIGIN      
        1 atgcttgatt taaatggcgg acaattattg ttattattaa gtctaatgtc atttaaaggc
       61 attatcatat ttacgaagag atcaggatga taacgatgaa aaaaagtgta ttgacggcgt
      121 ttataactgt ggtatgtgca acgtccagcg ttatggctgc tgatgataat gctatcacgg
      181 atggctcagt aacatttaat ggtaaagtta ttgctccagc ttgtaccctg gtagctgcga
      241 cgaaagattc cgtggtgact ttgccagatg ttagtgccac gaagttgcaa accaatggtc
      301 aggtttctgg cgtgcaaact gatgtgccaa ttgaattaaa agattgtgat actaccgtaa
      361 caaaaaatgc aacgttcacc tttaatggca ctgcggatac tactcagatt acagcgtttg
      421 ctaaccaggc ctcatctgat gctgctacaa acgtggccct gcaaatgtat atgaatgatg
      481 gtacaacggc catcaagcca gacacagaaa ccgggaacat tttgttgcaa gatggagatc
      541 agacgttgac ttttaaagtt gattatatcg ctacggggaa agcgacttct ggtaatgtga
      601 atgcggtaac aaatttccat attaactatt attaatagaa ctcattaatt gttttattaa
      661 ttagtacccc tccagtgtta tggaggggat attcatattt tttaagagtg actatttatg
      721 aaaacttgca taacaaaggg aattgtgacc gtaagtttaa cggcaatatt actcagctgt
      781 tcgtccacat gggcggcagg taaaggcggg gttggacttg cagcaacacg tcttgtttat
      841 tcggaaggtg aagagcaaat ttcactgggt gtacgtaaca ccagtccgga tgttccttat
      901 ctgattcagt catgggtgat gaccccagat aataaaaaat cagcggactt tattattaca
      961 ccaccgctat ttgtgctgaa tccggcaaac gagaatctgt tacgcattat gtacattggc
     1021 gcgccgttgg cgaaagacag agaaactctt ttcttcacta gcgtacgggc agtcccttca
     1081 acaacgaagc gggaagaagg caataccctg aagattgcca cacaaagcgt catcaaactt
     1141 ttctggcgac caaaaggttt agcgtatccc ttaggcgagg ctccggcgaa actgcgttgc
     1201 acttcgtcag ctgacatggt tacggtcagt aacccaacac cttatttcat taccctgaca
     1261 gacctgaaaa taggtggaaa gttagttaaa aatcaaatga tttccccctt tgataaatac
     1321 caattctctc tgccaaaggg tgccaaaaat agcagcgtaa cgtatcgaac catcaatgac
     1381 tacggggcgg aaacgccgca actcaactgt aaatcgtaat tcgtcttcag ttaagagagc
     1441 gagatgtata gaactcaccg acaacacagc ctgttaagct ctggtggagt gccatcgttt
     1501 attggtgggc tggtggtgtt tgtgtcggca gcgttcaatg cacaagctga aacctggttc
     1561 gatccagcct ttttcaaaga tgatccctca atggtggccg atttgtctcg tttcgaaaaa
     1621 ggacaaaaaa taacgccagg ggtttatcgt gtcgatattg ttctgaatca gacaattgta
     1681 gatacgcgca acgtcaattt tgttgagata acgccagaga aggggattgc cgcctgtttg
     1741 acgactgaaa gcctggatgc aatgggcgtg aatactgatg catttccggc ttttaaacaa
     1801 ctggacaaac aagcgtgtgc gccattggcg gagattattc cggatgccag cgtaactttt
     1861 aatgtgaata aactccgtct ggaaatttca gtaccgcaaa ttgccatcaa aagtaatgct
     1921 cgtggttatg tccccctgaa cgttgggatg aagggatcaa cgcgctatta ctgggatatt
     1981 catttagcgg ggctaacagt attcatagca gcgcagacag tgattctggc gacagttatt
     2041 ttctgaattt aaacagtggc gttaatttag gcccatggag attgcgcaac aattcaacat
     2101 ggagtcgtag tagtggccaa accgcagaat ggaagaatct cagcagctat ttgcaacggg
     2161 cggttattcc tctgaaaggc gaactgaccg taggtgatga ttatactgca ggcgattttt
     2221 tcgatagtgt cagctttcgt ggtgtgcagc tggcgtcaga tgacaacatg ctgccagaca
     2281 gcctgaaagg ggttgcgcct gtggtgcgtg gtatcgccaa aagcaatgcc cagataacga
     2341 ttaagcaaaa tggttacacc atttaccaaa cttatgtatc gcctggtgct tttgaaatta
     2401 gtgatatcta ttccacgtcg tcgagcggtg atttgttagt tgaaatcaaa gaagcggacg
     2461 gcagcgtcaa tagctacagc gtaccctttt ccagcgtgcc attactccag cgtcaggggc
     2521 gaatcaaata cgcggtgaca ctggcgaaat acagaaccaa tagtaatgaa cagcaggaga
     2581 gcaaatttgc ccaggccacg ttgcagtggg gcggaccgtg gggaacgacc tggtatggtg
     2641 gtggacaata tgctgaatat taccgtgccg ccatgtttgg tctgggattt aacctcggcg
     2701 atttcggagc aatttcgttc gatgtgaccc aggcgaagag tacgctggca gaccaaagcg
     2761 aacataaagg tcagtcatat cgttttctgt atgccaaaac gctcaaccaa ttgggtacta
     2821 actttcaatt gatgggctat cgctattcga cgtcgggttt ctacaccctt tccgacacca
     2881 tgtataaaca tatggatggc tacgaattta atgacggtga tgatgaagat acgccgatgt
     2941 ggtcgcgtta ttacaatttg ttttacacca aacgtggcaa actgcaggtc aatatctccc
     3001 agcaattaag cgagtacggt tcgttttatt taagtggtag ccagcaaact tactggcata
     3061 ccgatcaaca ggatcggcta ttacagtttg gctataacac gcaaattaaa gatctctcgc
     3121 tggggatctc ctggaactac agtaagtccc gtggccaacc tgatgctgat caggtgtttg
     3181 cacttaattt ttccctgcca ctcaatctgt tgctctccag aagtaatgat agctatacca
     3241 gcaaaaaaaa ttacgcctgg atgacctcta acaccagtat cgataacgaa gggcacacta
     3301 cacaaaacct gggtttaacg gagacactac tcgatgacgg taacctgagc tacagcgtgc
     3361 aacagggata taacagcgag gggaaaacgg ctaatggcag cgccagcatg gactacaaag
     3421 gggtgtttgc agatgcccga gtgggctaca actacagcga taacggcagt caacaacaac
     3481 tgaactacgc tctttcaggc agtttagttg cccattcaca gggcattacc ctggggcaat
     3541 cactggggga aactaacgtt ctgattgcag caccaggcgc agagaatact cgtgtggcga
     3601 acagcaccgg gctgaaaact gactggcgcg gatataccgt tgttccttat gccacttctt
     3661 atcgtgaaaa tcgaatcgca cttgatgcgg cgtcgttaaa acgtaacgtg gatcttgaaa
     3721 atgcagtagt caacgtggtt cccaccaaag gggcgttggt tctggcggag ttcaatgccc
     3781 atgcgggagc aagggtatta atgaaaacat caaagcaggg tatatcgctg cgttttggcg
     3841 cgatagcgac gctggacggc gtacagacta atagcggcat aattgatgat gatggctcgc
     3901 tctatatggc gggtttaccg gcgaagggaa caataaccgt gcgctggggc gaagctcccg
     3961 atcaaatttg tcatatcagt tacgagctta ccgaacaaca aattaactct gcgattacgc
     4021 gaatggatgc catatgcaga taatctttgg agaaaaatgc gtgtcattac tacgactatt
     4081 ttttgccgcc gtcttaatgc tatggtgcgc tcaaaccgct gcttatagcg ggcagtgtca
     4141 taccactcag gggaatccgt atattggcgt caattttggc gttaaaaccc tggaggaaga
     4201 agaaaatacg actggggtag taaaagacaa attttatcag tggaacgaat cgaatgatta
     4261 ttatgtttcc tgtgattgcg ataaagacaa tgtcagaagt ggccgatggg cattcgccgc
     4321 ggattcaccg ttagtctatt taggcgacaa ctggtacaaa attaatgact atcttgccgc
     4381 caaagtttta ttgcaggtta aaggcagttc tcctacagcg gttcctttcg aaaacgtggg
     4441 gactggggca gatacccggt ggcatatttg tgaccccggc ggtcaacgtt taggcggcca
     4501 gggagctagc ggtaatagcg gtagcttttc cctgaaaata ttgcagccgt tcgttggttc
     4561 ggtcgtcatt cctcctatgg cgctggcgcg attatttgaa tgctacaaca tacccgcagg
     4621 tgattcctgc acgactacag gcacaccggt tttagtgtat tacctgtctg gtactatcaa
     4681 ttcacttggc tcatgttccg tcaatgccgg agaaacaatc gaggtcgatc tgggcgacgt
     4741 atttgcggct aactttcgtg ttgtagggca taagcctctt ggggccagaa cggcagaact
     4801 tgcaattcca gtcaggtgta acacgggaaa cgcggggtta gttaacgtca acctgagtct
     4861 gacggcaacc acagacccca gctatcccca ggcgattaag acgtcacgtc ctggcgtggg
     4921 cgtggtggtg accgatagcc agaacaacat tatttcccct gctggtggaa cattaccgct
     4981 ctctattcct gatgatgcag acagtatcgc gcgaatgaat gtctatccag tcagcacgac
     5041 aggtgtacca ccagaaaccg ggcgatttga agccacggca acggtgagaa taaattttga
     5101 ttaacgcgtg aacgtatgaa gaaaaaaaac gatatttcag tgcgttatct tgttctttag
     5161 cattcttaac atccatgtcg ggatggctgg gcctgaacaa gttagtatgc atatttatgg
     5221 gaatgtggtc gatcagggct gtgatgtcgc caccaaaagt gcattacaaa atattcatat
     5281 tggtgatttt aatatcagtg attttcaggc cgcgaatacc gtaagcactg ctgctgattt
     5341 gaatatcgat atcaccggtt gtgccgctgg tattactggc gcggacgtcc tttttagcgg
     5401 cgaggctgac acccttgcgc cgacactgct caaactaact gacacaggcg gaagcggtgg
     5461 tatggcaacg gggattgccg tgcaaattct tgatgcgcaa agtcagcaag aaatcccgct
     5521 caatcaggtc cagcctctta cgcccttaaa agccggggat aacacactca aatatcaact
     5581 tcgttataag tccacaaagg cgggagcaac gggcggtaat gcgacggcgg ttctctattt
     5641 tgatctggtt taccagtgaa ggagtgatgt tgaaacggat aatctggatt ctgttcttac
     5701 tgggattaac gggggggtgt gagctatttg cccatgatgg cacggtcaac attagcggat
     5761 cgtttcgccg taatacatgc gtgctggcac aggatagcaa gcaaatcaat gtgcagttag
     5821 gcgatgtctc actgacacgt ttttctcatg ggaattatgg cccggagaaa tctttcatca
     5881 ttaatttgca ggattgcgga acagatgtca gcacggttga cgtcaccttt tcaggaaccc
     5941 ccgacggagt gcagagcgaa atgttgtcca ttgaaagtgg tacggatgca gccagcgggc
     6001 tggcaattgc gattctggat gatgcgaaga tattgattcc gcttaaccag gcgagtaaag
     6061 actacagcct acatagcggt aaggtgccat taacttttta tgcgcaattg cgacctgtca
     6121 atagcgatgt gcagtcgggt aaagtgaatg ccagcgcgac atttgtactt cattatgact
     6181 aatacgtgga acagattggc gctcttgatt ttcgccgttt tatcgttgct ggtggcgggt
     6241 gaattgcagg caggagtggt ggtcggcgga acgcgattta tctttccggc agacagagaa
     6301 tcgatatcta ttttactgac taataccagt caggaatcct ggcttattaa cagtaaaatc
     6361 aaccgcccaa cgcgttgggc cgggggtgaa gcgtcgacag tgccagcacc attactggcc
     6421 gctccgccac ttattctcct gaagcccggt acgacaggca cgttgcgctt gctgagaacg
     6481 gaaagcgaca tcttgcctgt ggatcgcgaa acgctatttg agttaagcat tgccagcgtg
     6541 ccatccggca aagttgaaaa tcagagcgta aaagtggcga tgcgctcggt atttaaactg
     6601 ttctggcgac ccgaaggttg ccgggcgacc cgctggaagc ttatcaacaa ttacgctgga
     6661 cacggaagtc gcagggtgta caactcacta acccaacgcc ttattacatt aacctgattc
     6721 aggtgagcgt aaatggtaaa gcgttaagta atgtgggagt tgtgccgcct aaaagccagc
     6781 gtcagacaag ctggtgtcag gcaatagcac cgtgtcatgt tgcctggcgg gcgataaatg
     6841 attacggcgg gttgagtgca aagaaggagc aaaatctgcc ctgaaacagg ttcggaaaac
     6901 gtttgcgttt tttttgccgc aggtcaattc ccttttggtc cgaactcgca cataatacgc
     6961 ccccggtttg cacaccggga atccaggaga gttcatgtac taccccttcg ttcgtaaagc
     7021 ccttttccag ctcgatccag agcgcgctca tgagtttact tttcagcaat tacgccgcat
     7081 taccggaaca ccgtttgaag cactggtgcg gcagaaagtg cctgcaaaac ctgttaactg
     7141 catgggcctg acgtttaaaa atccgcttgg tctggcagcc ggtcttgaca aagacgggga
     7201 gtgcattgat gcgttaggcg cgatgggatt tggatcgata gagatcggta ccgtcacgcc
     7261 acgtccacag ccaggtaatg acaagccgcg tctctttcgt ctggtagatg ccgaaggttt
     7321 gatcaaccgt atgggcttta ataatcttgg cgttgataac ctcgtagaga acgtaaaaaa
     7381 agcccattat gacggcgtcc tgggtattaa catcggcaaa aataaagata cgccagtaga
     7441 gcagggcaaa gatgactatc tgatttgtat ggaaaaaatc tatgcctatg cgggatatat
     7501 cgccatcaat atttcatcgc caaatacccc aggattacgc acactgcaat atggtgaagc
     7561 gctggatgat ctcttaactg cgattaaaaa taaacaaaat gatttgcaag cgatgcacca
     7621 taaatatgtg ccgatcgcag tgaagatcgc gccggatctt tctgaagaag aattgatcca
     7681 ggttgccgat agtttagttc gccataatat tgatggcgtt attgcaacca ataccacact
     7741 cgatcgttct ctggttcagg gaatgaaaaa ttgcgatcaa accggtggct taagtggtcg
     7801 tccgcttcag ttaaaaagca ccgaaattat tcgccgcttg tcactggaat taaacggtcg
     7861 cttaccgatc atcggtgttg gcggcatcga ctcggttatc gctgcgcgtg aaaagattgc
     7921 tgcgggtgcc tcactggtgc aaatttattc tggttttatt tttaaaggtc cgccgctgat
     7981 taaagaaatc gtt
//
LOCUS       DAZFAB010000161         7989 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZFAB010000161 DAZFAB010000000
VERSION     DAZFAB010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7989
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.161"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(142..999)
                     /gene="murI"
                     /locus_tag="ACHJWA_004397"
     CDS             complement(142..999)
                     /gene="murI"
                     /locus_tag="ACHJWA_004397"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFO4310860.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
     gene            complement(944..2788)
                     /gene="btuB"
                     /locus_tag="ACHJWA_004398"
     CDS             complement(944..2788)
                     /gene="btuB"
                     /locus_tag="ACHJWA_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFO4310861.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGGSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQPDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLVDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNIIIGHGNVGAG
                     VDWQKQSTAPGTAYVKDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     regulatory      complement(2853..3043)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3157..4257
                     /gene="trmA"
                     /locus_tag="ACHJWA_004399"
     CDS             3157..4257
                     /gene="trmA"
                     /locus_tag="ACHJWA_004399"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFO4310862.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     gene            complement(4297..4656)
                     /gene="yijD"
                     /locus_tag="ACHJWA_004400"
     CDS             complement(4297..4656)
                     /gene="yijD"
                     /locus_tag="ACHJWA_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFO4310863.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4656..5306)
                     /gene="fabR"
                     /locus_tag="ACHJWA_004401"
     CDS             complement(4656..5306)
                     /gene="fabR"
                     /locus_tag="ACHJWA_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFO4310864.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            complement(5410..6726)
                     /locus_tag="ACHJWA_004402"
     CDS             complement(5410..6726)
                     /locus_tag="ACHJWA_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate-proton symporter"
                     /protein_id="HFO4310865.1"
                     /translation="MTAIVNETVSVKKKPSGRRVIFASAFGNALEFFDFGVYNFFVVY
                     ISTLFFPPSADKNVALLLAFATFGVSFFMRPLGGIIVGAWADRFGRKPAMVFTIALMS
                     LGTLMIGIAPTYETAGYWGTATLVLARLIQGVAAGGEVGASMSLLVESAPANRRGFYS
                     SWSLATQGLATTFGGVVALGLSAWLPFATGSETVMAEWGWRVPFFIGVLLAPVGCWLR
                     LSLENDVPEPAHNKKAAASESAFSLLMQHKATIANGILLAIGSTVATYISLFYYGTWA
                     AKYLGMNQNYSHAAMLLAGVITFVGALLVGMLCDSVGRKKLILISRVMVLICSWPSFW
                     LLVNYPSPGMLLTVVFVMVSFTTLGGVPVMLLISELLPKRIRALGFALVYSIGVAIFG
                     GFAQYFATQSIVLLDSLTAPAWYLGGGTLLSMLALLYVKEPAKELQ"
     gene            complement(6769..7935)
                     /locus_tag="ACHJWA_004403"
     CDS             complement(6769..7935)
                     /locus_tag="ACHJWA_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HFO4310866.1"
                     /translation="MIEHYIRGFEEELREIRHQIHENPELGLQEFKTSALVAEKLRQW
                     GYEVEQGLATTGIVATLKVGDGEKSIGLRADMDALPIYENSGKPWASKHPGLMHACGH
                     DGHTTILLGAARYFAETRRFNGTLRLIFQPAEEMINGGEIMVKEGLFDHFPCDVIFGM
                     HNMPGLPVGKFYFQPGALMASMDQFHITVRGCGGHGAIPHKAIDPVLVAAHITTALQS
                     IVSRNVDPLEAAVITVGSIVAGEAANVIPDSAEMKISVRSLSRDTRQLLLTRIPALAQ
                     AQAASFGATAEVTHVNGTPVLVNDEEMARFAWQVACKTFGEDRAEFGIKPLMGSEDFS
                     FMLEAQPKGGFLLFGNGDVGEGSCMVHNPGYDFNDASLVPASSYWGALVEAWLQ"
BASE COUNT         2091 a         2172 c         1897 g         1829 t
ORIGIN      
        1 cggaattctc aggagaaccc cgctgacccg gcggcgtgtt tgccgttgtt ccgtgtcagt
       61 ggtggcgcat tatagggagt tattccggcc tgacaagagg aaatttaaaa taattttccg
      121 accgcgcaac attcaaccaa atcagcctaa aactgccagt ttttcgagcg tttcgaagcc
      181 gtaacgctgt aaaacgggca ataattgttc agcttctggc gtcattgcca tacaaaaggc
      241 aatattcgca tcggcagatt ttgcatccgg ggcttcatgt tctaacaacc aggccgttcg
      301 gcgagcaatc gctgcgccag aatccaccag ccgggttccc tctggcagca cttgtaacag
      361 ttcttcttgt agtagaggga aatgggtgca acccaataca acggtatctg gcggctcttt
      421 cattcttaac cacgggcgta ggatacgttt tagtgcatcc agagaaacat cttcgccatg
      481 cagcttcgct tcagccaact caaccatctc tgccgagccc agcatttcta tctggcattc
      541 attagcgaaa cgcgcgatca gctcatgagt ataagaacgt ttaactgttc cgcgggttgc
      601 cagtaatccg acaatgccat ttgccgtcag acgtgcagca ggtttaatcg ccggcacgac
      661 accaacaacc gggaagtcga acttttcgcg taatgcagga agtgaaacgg tactggcagt
      721 gttgcaagcg accacagcca gcgcaagggg ataacgttct tgcaccgcgg tgacaattgc
      781 caccactcgc tcaacaataa acgcttcgct tttttcgcca tacgggaaag cgacgttatc
      841 gaaagcataa atgtaatgga gatccggtaa gagatgccgg atctcgtcat agaccgacaa
      901 cccaccgacg ccggagtcaa acaccagcac ggtgggacgt ggttcagaag gtgtagctgc
      961 cagacaaggt gtattcccgt cctgcagttt ggtagccata gactgtctca taatctttgt
     1021 cgaacaggtt ggctatttta ccacgaactg tcaggtgaga ggtgaccgga tacgcaaccg
     1081 caagatccca caagctcaca ccgcccattt taacggtttg ataaggataa gatgagtaat
     1141 ccttatcata gcgagtgcct aaatactgat aagtaatacc ccagtcgaag tcatacaact
     1201 gccagtcgag ctggtatttc acctgctgtt tagcacggcg taacaacggc gtgtcggtaa
     1261 ttgcattgcg cgcatcgaca taatcataac tcacagtatg cgtcagtggt ccggtatcaa
     1321 aattggcggt cgcctcgacg cccttaatcc gcgctttccc ttcgttgtaa tatttcaggg
     1381 tgtgatcatc ataatcgatc aagtcactga catcgttacg atatccggaa atacgccagt
     1441 tcaccccagc ggttaagcct tcaaacgcgc cttcccactg tttgcttttc tccgggtcca
     1501 gattcggatt tccgtagaag ccatacagtt gccccagatt tggtgcctta taagatgtcc
     1561 cgtaggaagc aatgaagcga taaccttcga tgaattccca accggcgctg gtttgccagg
     1621 ttccatgacg accaaactgt gagttatcgt cgctgcgtgc tgcgccttca aaggtaaaat
     1681 cgccgacttg ttgcagcccg gtcagataga tgccggtatt acgttgatca tatccatcct
     1741 taacataagc tgtgcccggt gccgtgctct gcttctgcca gtcaacaccc gcaccaacat
     1801 taccgtggcc aatgatgatg ttgtttgccc actggacggt gtattgcttc atctcatcga
     1861 gcgtcgccga cgaatcataa cgaccataat ggggatcgta gttgtaatct ttgctatggc
     1921 tatagctggt aatgagttgt gatttaatca gttcgccgtt atatcgcagc ccggcgtccc
     1981 aactttgact atagagttta cgggtatcga ccaatggtga acccggagaa taatacgcgt
     2041 cataattggt acggttatca tagccatagc cgcgcacaaa gccgctccag gcatcagtaa
     2101 agttatgctc cagcgcgcca taaagcgttt tacttaaaaa accatcgtta tctggctgcg
     2161 cttgcgttcc ggtattacca taggcaacaa catcataacc atgagtatgg gcataatcgc
     2221 ccaacaacgt tactcgtgtc ttatccccca gttgttgctg tgtagagaca tcatagtttt
     2281 gataactatt gcttccccac cctgctgaaa tttccgttcc gggttcatcg cgcgtcgtga
     2341 tgatattcac caccccacct attgcatcgg aaccataaac ggcggagcgt ggcccacgga
     2401 tatattcaac acgctggaca agcgcaatag ggaactggct aaggtcggca gaaccactcc
     2461 cccccgccag attcaggcgt acgccatcaa ttaacaccaa cacatgactg gcatttgtac
     2521 cgcgaataaa aatagatgag agctgacctg aaccgccgtt ttgggtgata tcgacgcccg
     2581 gaagacggcg cagcacatca ttaaccgagg tcgactgcca gcggtcgata tcctgacgcg
     2641 tcacaacggt ggttggtgca agcacagtgc tgcgcggctg ttcaaaacgg ttagcagtaa
     2701 cgacgagagt atccgggctg gtatcctgtg cccaagcgga aaaggctgtg acagaacacg
     2761 ccgtcagcag cgaagctttt ttaatcattg taaagcatcc acaatagaag aaggatgccg
     2821 caggtttcat caatattacg cgatgatgag aaccagatgc gacgttggcc ggcaggtctt
     2881 cgggcttgga ggggtatcta agatactaag agatgatgac ttcccaccga atggcagtgt
     2941 ccgcataacg caatcatcgc acctttcctt accgctgcgc gtcagctcca gattcgcact
     3001 ggattcccta ttaactcaca ggaccggcaa gtggatgcta caggttgtaa caagttactg
     3061 tccagacgta gctcacaaat aggaattcat caagatctgg acatctgatg agcaatccct
     3121 acaatcgccg cgtactttaa tttttcagga tacatcatga cccccgaaca ccttccaaca
     3181 gaacagtatg aagcgcagtt agccgaaaaa gtggtacgtt tgcaaagtat gatggcaccg
     3241 ttttctgacc tggttccgga agtgtttcgc tcgccggtca gtcattaccg gatgcgtgcg
     3301 gagttccgca tctggcacga tggcgatgac ctgtaccaca tcattttcga tcaacaaacc
     3361 aaaagccgta tccgggtgga tagcttcccc gccgccagtg aactcatcaa ccagttgatg
     3421 acggcgatga ttgcgggtgt gcgtaataat cccgttctgc gccacaagtt gttccagatt
     3481 gattacctca ccacgctgag taatcaggcg gtggtttccc tgctgtacca taaaaagctg
     3541 gatgatgagt ggcgtcagga agcggaagcc ctgcgcgatg cactgcgcgc gcagaatctg
     3601 aatgtgcatc tgattggtcg ggcaacgaaa accaaaatcg cgctggatca agattacatc
     3661 gacgagcgtc tgccggtcgc agggaaagag atgatctatc gtcaggtaga aaacagcttt
     3721 acccagccga acgcggcgat gaatattcag atgctggaat gggcgctgga cgtaaccaaa
     3781 ggctcaaaag gcgatttact ggagctgtac tgcggcaacg gtaacttctc attagcgctg
     3841 gcgcgcaatt ttgatcgggt attagccacc gaaatcgcca agccatcagt cgctgccgcg
     3901 caatacaaca ttgccgctaa ccatattgat aacgtacaaa ttattcgtat ggcggcagaa
     3961 gaatttactc aggcgatgaa tggcgtgcgc gagtttaacc gcctgcaagg gatcgactta
     4021 aagagttatc agtgcgaaac catttttgtc gacccacctc gcagcggtct ggacagtgaa
     4081 accgagaaaa tggtgcaggc gtatccacgt attctgtata tctcctgcaa tccggaaacg
     4141 ttatgcaaaa atctggaaac attaagccag acgcacaagg tcgaacgtct ggctctgttt
     4201 gatcagttcc cctacacgca ccatatggag tgcggcgtat tactgaccgc gaagtaaaac
     4261 ccatgccgga tgcgccagca tccggcatga taccgattac tcagcaactt cctgcttacg
     4321 gttacgcatc ttcgcgccaa tccagaacac cataatgacg gagagtactg ccgggaagaa
     4381 gttagagccg atatccggat attccgcacg aactaccgtg ctgtacagca gtacgccgag
     4441 aataaaacag gcagctgcca gacccggcaa gcctaccggc atggtgcgat taagataacg
     4501 ttgatgcagg cagtaaaccg tcagcaccag ggaaataatc gggaatacag aaaatggcac
     4561 aatggagcta aacagcgctg cgaaagtacc attaatcgat aagccagcaa ccaacgccag
     4621 cagcagcgta cctctatctt gatttgcttg tttcattact cgtccttcac atttcccgga
     4681 ataattgcgg ttttctcttg ttcacggcga taccagtaat aagccccttt cgaaatcatt
     4741 cgcagttgca gtaccagtcg ctcttctaat tgccgacgtt gttcaacgcc gacgtccaac
     4801 gcctcggcac ccgcactgaa gacaattgtc accattgctt cggcttgcgc ttcagtaaac
     4861 gcacgcggca tatggttttc gagttccaga tagtccgcaa gttccgcaat gaagtgctga
     4921 atttcacgcg caacggcggc acgaaacgca gcggaggtgc cggagcgttc ccgcaataat
     4981 aaccggaagg cgttaggatt attaccgatg aactccataa atgtggagac cgaggtgcgg
     5041 atcacactcc cgcctttggc gatacgctga cgcgcctggc gcatgagttg gcgtagcatt
     5101 aaaccgctct catcaaccat ggtcagaccc agttcgtcta cgtctcggaa atgccgataa
     5161 aaagaggtgg gagcaatgcc cgcttcacgc gccacttcac gcaaactcag gctggcgaag
     5221 ctgcgttcag cacttaattg gctaaatgcg gcttccacca gcgaacggcg ggttttttct
     5281 ttttgttgcg ctcttacgcc catcacgatg tctgaatcct tgccaaaagt actgctggca
     5341 ctataccaga gaatgaacat actggattgc tccagtattg tgaatgaacg gtaacgcaat
     5401 aaaacatatt tactgcaatt ctttggccgg ttcttttacg tacaacaacg ccaacatcga
     5461 cagcaacgtg cctccaccca gataccacgc cggagccgtc aaactatcca gcaacacgat
     5521 ggattgcgta gcgaaatatt gagcaaaacc gccaaagatt gccacaccaa tgctataaac
     5581 cagcgcaaaa cccaatgccc gaatccgttt cggcaacagt tcagagatca acaacatcac
     5641 tggcacaccg ccaagcgtgg taaagctgac catcacgaaa accaccgtca gcaacatgcc
     5701 tgggcttggg taattcacca acagccagaa tgacggccag ctacagatca gcaccatcac
     5761 acgggagatt aaaatcagct ttttacgccc gaccgaatcg cacaacatcc ccaccagcag
     5821 cgcacccaca aacgtaataa cgcccgccag taacatcgct gcatgagaat agttttggtt
     5881 cattcctaaa tacttcgccg cccatgtacc gtaataaaag agggagatat aggttgcgac
     5941 tgtactacca atagcgagca agataccgtt agcgatagtc gctttatgtt gcataagtaa
     6001 ggagaaggcg ctttcgctgg ctgcggcctt cttattatgt gctggctcag gaacatcatt
     6061 ttccagactc aaacgcaacc aacagccgac aggcgctaat aacacgccaa taaaaaacgg
     6121 cacacgccag ccccattcag ccatgacggt ttctgaaccg gtcgcaaagg gcagccatgc
     6181 acttaaacct aaagcgacaa cacctccaaa agttgttgcc aggccttgag tcgccagtga
     6241 ccaactgctg taaaaaccgc gacgatttgc cggagctgat tcaaccaaca aagacattga
     6301 ggcaccgact tcgcctcctg ccgcaacgcc ctgaattaga cgcgctagca ccagcgtcgc
     6361 ggttccccaa tagcccgccg tttcatacgt cggtgcgatg ccgatcatca gtgtaccaag
     6421 actcatcagc gcaatggtga acaccatcgc gggtttgcgt ccgaaacgat cggcccaggc
     6481 ccccacaata atgccgccga gcgggcgcat aaaaaagctc acgccgaaag tggcaaacgc
     6541 cagcagtagc gcgacgtttt tatctgcact gggcggaaaa aatagcgtgc tgatatagac
     6601 cacaaaaaag ttgtaaacgc caaaatcaaa aaattcgagc gcattgccaa acgccgaggc
     6661 aaaaatcacc cgcctgccgc tgggcttttt cttcacactc acggtttcat ttacgatcgc
     6721 cgtcattcca tgttccttgc caaaaagtgt tgttgtgtgt tgttttattt attgcagcca
     6781 ggcttctacc agtgcgcccc agtagctgct tgccgggacc agacttgcat cgttgaagtc
     6841 gtaacccggg ttatgcacca tgcaggaacc ttccccgaca tcgccattac cgaataacag
     6901 gaaaccgcct ttgggttggg cttccagcat gaaagagaaa tcttcactgc ccatcagggg
     6961 cttgatgcca aactcggccc gatcttcacc aaacgtttta cacgccacct gccaggcgaa
     7021 gcgcgccatc tcctcgtcat ttactaatac tggtgtaccg ttaacatgcg taacctcagc
     7081 cgtagcgcca aagctggcag cctgggcttg tgccagagca ggaatacggg tcagaagaag
     7141 ttgtcgggta tcgcggctaa gagagcggac gctgattttc atctcagcac tgtcaggaat
     7201 gacgttagcc gcctcacccg caacaatgct gccgacggta attaccgccg cttccagcgg
     7261 atcgacattg cgtgacacaa tgctttgtaa tgcggtggtg atatgtgcgg caactagcac
     7321 cggatcgatg gctttgtgcg ggatcgcacc gtgtccgcca caaccacgaa ctgtaatatg
     7381 gaactgatcc attgacgcca tcagtgcccc tggctggaaa taaaacttac ccaccggcag
     7441 accgggcatg ttatgcatgc cgaaaatgac atcgcagggg aaatgatcaa aaagcccctc
     7501 tttaaccatg atctcaccgc cattaatcat ctcttccgca ggctggaaga taagacgtaa
     7561 cgtgccgtta aaacggcggg tttcggcaaa ataacgcgcg gcccccagca gtatggtggt
     7621 atgcccgtca tgcccgcagg cgtgcattaa accaggatgt ttactggccc agggcttgcc
     7681 actattttca taaatcggca gcgcatccat atccgcccgc aggccaatac ttttctctcc
     7741 gtcgcccact tttaacgtgg ccactatccc cgtcgtcgcc agtccttgct ctacttcata
     7801 gccccactga cgcagctttt ccgcaaccaa agcactggtt ttaaattctt gtaatcccag
     7861 ctccgggttt tcgtggatct gatggcggat ctcacgcagt tcttcctcaa accctcgaat
     7921 atagtgttcg atcatcggct attcctttat tcacttataa acaggctaat taacggattt
     7981 tattgatgg
//
LOCUS       DAZFAB010000162         7735 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZFAB010000162 DAZFAB010000000
VERSION     DAZFAB010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7735
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.162"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(193..336)
                     /gene="ypaB"
                     /locus_tag="ACHJWA_004404"
     CDS             complement(193..336)
                     /gene="ypaB"
                     /locus_tag="ACHJWA_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFO4310867.1"
                     /translation="MALLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVINNCS
                     AIS"
     gene            464..2749
                     /gene="nrdA"
                     /locus_tag="ACHJWA_004405"
     CDS             464..2749
                     /gene="nrdA"
                     /locus_tag="ACHJWA_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO4310868.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            2895..4025
                     /gene="nrdB"
                     /locus_tag="ACHJWA_004406"
     CDS             2895..4025
                     /gene="nrdB"
                     /locus_tag="ACHJWA_004406"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO4310869.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDSEVDTDDLSNFQL"
     gene            4025..4279
                     /gene="yfaE"
                     /locus_tag="ACHJWA_004407"
     CDS             4025..4279
                     /gene="yfaE"
                     /locus_tag="ACHJWA_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFO4310870.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4333..4983)
                     /gene="inaA"
                     /locus_tag="ACHJWA_004408"
     CDS             complement(4333..4983)
                     /gene="inaA"
                     /locus_tag="ACHJWA_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFO4310871.1"
                     /translation="MAVSAKHDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVTPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(5064..6254)
                     /locus_tag="ACHJWA_004409"
     CDS             complement(5064..6254)
                     /locus_tag="ACHJWA_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4310872.1"
                     /translation="MKEKLWTKDFWAITIISFIIFFVFYVLLTLLPIYISDRLHASPD
                     KAGLLVTLFLIAAIVIRPFAGQWVGKYSNKTILVLSSLAFLVVTALYPFCHSIESLLF
                     IRVLHGITFGVITTVKGTISARLIPASRRGEGISFFSLAMGLAMVVGPWIGLNMARWE
                     AFNMAFWLCTGVAAVGIILSLIMTVPPVISHADGSKPKMGFAAMFDRAALPFAMVTFF
                     MTFSYAGVSAFLALYARELNLMSAASNFLLCYAIFLMICRTFTGNVCDKKGPKYVVYP
                     CLLFFTVGLVVLGYTQGSVMMVVSGALIGIGYGSVTPVFQTQIISSVEPHKIGVANSL
                     FFNAMDAGLALGACVMGMMVAHTGYRMIYLLGALLVVVAGGVYALQMKGKSGVALVVA
                     KEIH"
     gene            6406..7284
                     /locus_tag="ACHJWA_004410"
     CDS             6406..7284
                     /locus_tag="ACHJWA_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4310873.1"
                     /translation="MTLTQINSLLAVLDYGGFTEASKRLFMTQSAVSQAIAALEQELG
                     VNILLRDRRKAIQLTTAGHRIVQHLRAINREVNAVKEIAEQEKQNPQRTLRLGCFPSV
                     CACILPVVIRYFEIHHPNIKIIPFEENSTAIIDSLQNESIDAGFVHFPVSGMYSVPIY
                     QDKFTVVLPPNHALAKNSTITVEELMGEPLIISKGRYELSIMALFKEKNITPQIKYEF
                     NHPDTAISFIRQGLGIALLPELTLKTIADELCSVPLEPTFYRQISLLAKEKPVEGSPL
                     FLLQMCTEQLVVSGKI"
BASE COUNT         2084 a         1991 c         1808 g         1852 t
ORIGIN      
        1 tggcttcgca tttaacaata accgaataga aaacaaccat ttcgacatca acaatcgctt
       61 acattcgctt atatattgac cacaactgat acatcagatt atgtgatgac tcgtgcttag
      121 atcaattttt gcaatcatta gcaaaaagat taataaacca tctatatcaa tttatccaac
      181 ctattctgcc gttcaagaaa tcgccgaaca gttatttata acaaattttt ctcttcccat
      241 tgactttccc ggacaccttg tctgacctaa ggtgcgcgaa agccactttt tccttcctga
      301 gttatccaca aagttatgca cttgcaagag ggccattttc acactatctt gcagtgaatc
      361 ccaaacatac cccctatata tagtgttcta agcagcttcc cgtactacag gtagtctgca
      421 cgaaactatt gcggaaagaa ttccaaaaac aggtacgaca tacatgaatc agaatctgct
      481 ggtgacaaag cgcgacggta gcacagagcg catcaatctc gacaaaatcc atcgcgttct
      541 ggattgggcg gcagaaggac tgcataacgt ttcgatttcc caggtcgagc tgcgctccca
      601 cattcagttt tatgacggta tcaagacctc tgacatccac gaaaccatta tcaaggctgc
      661 cgcagacctg atctcccgtg atgcgccgga ttatcagtat ctcgccgcgc gcctggcgat
      721 cttccacctg cgtaaaaaag cctacggcca gtttgagccg cctgcgctgt acgaccacgt
      781 ggtgaaaatg gtcgagatgg gcaaatacga taatcatctg ctggaagact acacggaaga
      841 agagttcaag cagatggaca cgtttatcga tcacgaccgt gatatgacct tctcttatgc
      901 tgccgttaag caactggaag gcaaatatct ggtacagaac cgtgtgaccg gcgaaatcta
      961 cgaaagcgcc cagttccttt atattctggt tgctgcgtgc ctgttctcga actacccgcg
     1021 tgaaacgcgc ctgcaatatg tgaagcgttt ttacgacgcg gtttccacat ttaaaatttc
     1081 gctgccgacg ccaatcatgt ccggcgtgcg taccccgact cgtcagttca gctcctgcgt
     1141 actgatcgag tgcggcgaca gcctggattc catcaacgcc acctccagcg cgattgtgaa
     1201 atatgtttcc cagcgtgccg ggatcggcat caacgccggg cgtattcgtg cgctgggtag
     1261 cccgattcgc ggtggcgaag cgttccatac cggctgcatt ccgttctaca aacatttcca
     1321 gactgcggtg aaatcctgct ctcagggcgg cgtgcgcggc ggtgcggcaa cgctgttcta
     1381 cccgatgtgg catctggaag tggaaagcct gctggtgttg aaaaacaacc gtggtgtgga
     1441 aggcaaccgc gtgcgtcata tggactacgg ggtacaaatc aacaaactga tgtatacccg
     1501 cctgctgaaa ggtgaagata tcaccctgtt cagcccgtcc gacgtaccgg ggctgtacga
     1561 cgcattcttc gccgatcagg aagagtttga acgcctgtat accaaatatg agaaagacga
     1621 cagcatccgc aagcagcgtg tgaaagccgt tgagctgttc tcgctgatga tgcaggaacg
     1681 tgcgtctacc ggtcgtatct atattcagaa cgttgaccac tgcaataccc atagcccgtt
     1741 tgatccggcc atcgcgccag tgcgtcagtc taacctgtgc ctggagatag ccctgccgac
     1801 caaaccgctg aacgacgtca acgacgagaa cggtgaaatc gcgctgtgta cgctgtctgc
     1861 tttcaacctg ggcgcaatta atagcctgga tgaactggaa gagctggcaa ttctggcggt
     1921 tcgtgcactt gacgcgctgc tggattatca ggattacccg atcccggccg ccaaacgtgg
     1981 agcgatgggt cgtcgtacgc tgggtattgg tgtgatcaac ttcgcttact acctggcgaa
     2041 gcatggtaaa cgctactccg acggcagcgc caacaacctg acgcataaaa ccttcgaagc
     2101 gattcagtat tacctgctga aagcgtctaa tgagctggcg aaagagcaag gcgcgtgccc
     2161 gtggtttaac gaaaccactt acgcgaaagg gatcctgccg atcgatacct ataagaaaga
     2221 tctggatgcc atcgctaatg aaccgctgca ttacgactgg gaagctctgc gtgagtcaat
     2281 caaaacacac ggcctgcgta actccacgct ttctgctctg atgccgtccg agacttcttc
     2341 gcagatctct aacgccacta acggtattga accgccgcgc ggttacgtca gcatcaaagc
     2401 gtcgaaagac ggtattctgc gccaggtggt gccggactac gagcatctgc acgacgccta
     2461 tgagctgctg tgggaaatgc cgggtaacga tggctacctg caactggtgg gtatcatgca
     2521 gaaatttatc gatcagtcga tctctgctaa caccaactac gatccgtcac gcttcccgtc
     2581 aggaaaagtg ccgatgcagc agttgctgaa agacctgctc accgcctaca aattcggggt
     2641 caaaacgctg tattatcaga acacccgtga cggcgctgaa gacgcgcagg acgatctggt
     2701 gccgtcaatc caggacgatg gctgcgaaag cggcgcatgt aagatctgat attgagatgc
     2761 cggatgcggc gtaaacgcct tatccggcct acggctcggt ttgtaggcct gataagacgc
     2821 gccagcgtcg catcaggcac aggatgcggc gtaaaatgcc ttatccggca ttaaactccc
     2881 aacaggacac actcatggca tataccacct tttcacagac gaaaaatgat cagctcaaag
     2941 aaccgatgtt ctttggtcag ccggtcaacg tggcccgtta cgatcagcaa aaatatgaca
     3001 tcttcgaaaa gctgatcgaa aagcagctct ctttcttctg gcgtccggaa gaagttgacg
     3061 tttctcgtga ccgtatcgat taccaggcgc tgcctgaaca cgaaaaacac atctttatca
     3121 gcaacctgaa atatcagacg ctgctggact ccattcaggg tcgtagcccg aacgtggcac
     3181 tgctgccgct gatctcgatc ccggaactgg aaacctgggt agaaacctgg gcgttctcag
     3241 aaacgattca ttcccgttcc tatactcata tcattcgtaa tatcgttaac gatccgtctg
     3301 ttgtgtttga cgatatcgtc accaacgagc agatccagaa acgtgcggaa gggatctcca
     3361 gctactacga tgagctgatc gaaatgacca gctactggca cctgctgggc gaaggcaccc
     3421 acactgttaa cggtaaaacc gtgaccgtta gcctgcgcga gctgaagaaa aaactgtatc
     3481 tctgcctgat gagcgttaac gcgctggaag cgatccgctt ctacgtcagc tttgcttgtt
     3541 cctttgcctt tgcagaacgc gaactgatgg aaggtaacgc caaaattatt cgcctgattg
     3601 cccgcgacga agccctgcac ctgaccggta ctcagcatat gctgaatctg ctgcgcagcg
     3661 gtgcggatga tccggagatg gcggaaattg ccgaagaatg taagcaggag tgctatgacc
     3721 tgtttgtcca ggcggcacag caagagaaag actgggcgga ttatctgttc cgtgacggtt
     3781 cgatgattgg tctgaataaa gacattctct gccagtacgt tgaatacatc accaatatcc
     3841 gcatgcaggc agtcggtttg gatctgccgt tccagacgcg ctccaacccg atcccgtgga
     3901 tcaacacctg gctggtgtct gataacgtgc aggttgctcc gcaggaagtg gaagtcagtt
     3961 cttatctggt cgggcagatt gactcggaag tggacaccga cgatttgagt aacttccagc
     4021 tctgatggct cgcgttaccc tgcgcatcac tggcacacaa ctgctgtgcc aggatgaaca
     4081 cccttccctt ctggcggcgc tggaatccca caatgtggcg gttgagtacc agtgccgcga
     4141 aggttactgc ggctcctgtc gcacacgcct ggttgcaggc caggtggact ggattgccga
     4201 accgttagcc tttattcagc cgggggaaat tttgccctgt tgttgccgag caaaaggcga
     4261 tattgaaatc gagttgtgat cgggtactag cgccagatac gacgcttacg cgtattatct
     4321 ggcctacaac gattacatgg cgtagtaata cgcttttacc tgctcccagt ccgctttggg
     4381 gatcggctcc agatattttt ccagctggcg gaagtcatga ttaatcgctt tatcgcgacg
     4441 caagcggcga cgacttttct ccagatcaag aaaaccagct tctgccttac cttctgtttt
     4501 cacatagata tggcgaacat aacaacagcc atgctgacga ttaacgctat gcattttctt
     4561 aaacgccagc gccactgctt tcaacatggc ttgccgtact tcgtcagaat agggcgttac
     4621 tgcatgctgg gcataccagt cagcaatgct gatgaacccc gccatatctt cagtcaccag
     4681 caacgcgcgc cattccccct caattttcac cgcttcacca aaaacgatct tcggcacgat
     4741 gacaccggcc cgttccagtt ctttaattac cgcaacctca cggacaatcg ttggtcggcc
     4801 gaaaggataa cgtacggaat gaaacagatg atgcgtcatg cgctttacat atagcttttt
     4861 gccgttgcgc tcgacgcatt gcaccccgct cataccatta cggcgatagt taggctcttc
     4921 aacccagtcg ccctctgttg cccaccagtg attaaattcg tcgtgctttg ctgaaactgc
     4981 cataccctat cgcctgtcat tttttattaa cgacaatgac tataggtggt tacctgcgaa
     5041 aaatcttaat gaaacgtgtc gtattaatga atttcttttg ccactactag cgcgacaccg
     5101 ctttttccct tcatttgcag cgcatagact ccaccagcca ctaccactaa tagtgcgccc
     5161 agcagataaa tcattcggta gccagtatgt gcaaccatca tccccatcac acaggctccc
     5221 agcgccaggc ctgcatccat cgcattgaag aagagggagt ttgcgacacc gattttatgc
     5281 ggttccactg aactgataat ctgcgtctga aaaactggcg tcacggaacc atacccgata
     5341 ccaatcaacg cgccagaaac gaccatcatt acgctgccct gggtgtagcc gagaaccacc
     5401 agcccaaccg taaagaacag caggcagggg taaaccacat atttcgggcc ttttttgtcg
     5461 caaacattgc cggtgaaggt acggcagatc atcaggaaga tggcgtagca gagcaggaaa
     5521 ttactggccg ccgacatcag attaagttcg cgggcgtaaa gcgccagaaa ggcagaaacc
     5581 ccggcatacg aaaaggtcat aaagaatgta accatggcaa acggcaatgc cgcgcgatcg
     5641 aacatggcgg cgaagcccat ctttggcttt gaaccgtcgg catggctgat aaccggcggc
     5701 acggtcataa tcagcgacag gataatcccc accgccgcaa cgccagtaca taaccagaaa
     5761 gccatattaa aggcctccca gcgcgccata ttcaggccaa tccacggccc gaccaccatt
     5821 gccagcccca ttgccagaga gaaaaaactg atgccctccc cacgtcggga ggccgggatc
     5881 agccgcgcgg aaatcgttcc ctttaccgtt gtgataaccc cgaaggtaat accatgaagc
     5941 accctaataa aaagcagtga ttctattgag tggcaaaaag gatacagcgc agtgaccacc
     6001 aaaaaggcca gagaagagag caccagaata gttttattcg aatatttacc cacccattgc
     6061 ccggcaaagg gtcgaataac aatcgccgca attaaaaata aagtcaccaa caaacctgct
     6121 ttatcaggag aggcatgcaa gcggtcagag atataaattg gcaacaatgt tagtaaaaca
     6181 taaaagacga agaaaataat aaagctgatg atggttattg cccaaaaatc cttcgtccat
     6241 aacttctctt tcatactcta ccgcctgtca ggatgacgca cgcagacaac actcccgtta
     6301 ctgcggttgc gtcaaattgt gatgtacttt gcagaacaga agtataaatg gcggcatgct
     6361 attagagaaa ttaatcgttt taatattatt gattagcaaa ctctaatgac tcttacccaa
     6421 ataaattctt tactcgccgt gctggattac ggtggcttta ctgaagccag caagcgattg
     6481 ttcatgacgc agtcggcggt aagtcaggca attgctgccc tcgaacaaga acttggtgtg
     6541 aatatactgt tacgcgatcg ccgtaaagct atacaactga ccacagcagg tcaccgcatt
     6601 gtgcaacatc tgcgcgccat taatcgggaa gttaacgcag taaaagagat agccgagcag
     6661 gaaaagcaga accctcaaag aacgctgaga cttggttgtt tccccagcgt ctgcgcctgt
     6721 atcttaccgg ttgtgattcg ctactttgaa attcatcatc cgaatataaa aattattcct
     6781 tttgaggaaa atagcacagc gattattgat tcgcttcaga atgaatccat tgatgccggt
     6841 tttgttcatt ttccggtgag cggaatgtac tccgtgccca tttatcagga taaattcacc
     6901 gtagtgttac cgcccaacca tgctctggca aaaaacagca ctattacagt ggaagaatta
     6961 atgggtgaac cattaatcat tagtaaagga cgctatgaat taagcattat ggcactattt
     7021 aaagaaaaga atattacccc acagataaaa tatgaattta atcacccgga tacagcgatc
     7081 agttttattc gacagggatt aggcatcgcc ttattaccag agttaacgct gaaaaccatt
     7141 gcagatgaat tgtgttccgt tcctctcgaa ccaaccttct atcgacagat ttcgttgctg
     7201 gctaaagaaa agccggtaga aggcagtcca ctgtttttac tacaaatgtg tacggaacaa
     7261 ctagtggtga gtggaaaaat ttgatgtaag acatgagatt gatgacaaat gcaagactgc
     7321 ctgattcgcc acacttagtt gtactatagc ctttaccagg cgtaataatt cgttttcacc
     7381 tgattttggc ccactttgtg aattggcgat atatttatcc agttgggaga agtgacaatg
     7441 actccaggta gaaaccggta tgaagcgccc gactcactgt caaaaccgat tgttgagagt
     7501 aatgacactc cggcgaacag tacacagttt actctggagc cattctcaat cttcaagtgg
     7561 gatacctaat aacttgtcag ggaagttatc aatatctgtt tgttacttat taccgtattt
     7621 attaacaata atgatacttt ctaacatgcg taaaggtctt cgtaataaaa tgagctaaat
     7681 ttgatagtga atatattctg caaagattaa attgtatctt gttaactcac aatac
//
LOCUS       DAZFAB010000163         7531 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZFAB010000163 DAZFAB010000000
VERSION     DAZFAB010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7531
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.163"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            12..1034
                     /locus_tag="ACHJWA_004411"
     CDS             12..1034
                     /locus_tag="ACHJWA_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HFO4310874.1"
                     /translation="MSLTRRRFTQILASTLFLHHLPSFAQSVKFWASLTLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLSEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETWISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASRFVGEAQAFAASPAANLRFYAARGPRGLETGLKGSLHTEAAELLG
                     LHNVAQIADRQGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWHGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAT"
     gene            1034..2014
                     /locus_tag="ACHJWA_004412"
     CDS             1034..2014
                     /locus_tag="ACHJWA_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFO4310875.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENVPV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACTA
                     ILWGLSIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFVLIVCATLITASTVAIAGIIGWVGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            2011..2769
                     /locus_tag="ACHJWA_004413"
     CDS             2011..2769
                     /locus_tag="ACHJWA_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO4310876.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEVLLDSLPVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLAARRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISH"
     gene            2779..3591
                     /locus_tag="ACHJWA_004414"
                     /pseudo
     CDS             2779..3591
                     /locus_tag="ACHJWA_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903984.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            3588..4442
                     /gene="modD"
                     /locus_tag="ACHJWA_004415"
     CDS             3588..4442
                     /gene="modD"
                     /locus_tag="ACHJWA_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HFO4310877.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQHGYIEFFLRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAIQNVLEW
                     SCGVSDYLDQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQDWSGAINQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPAASI"
     gene            4468..6438
                     /locus_tag="ACHJWA_004416"
     CDS             4468..6438
                     /locus_tag="ACHJWA_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFO4310878.1"
                     /translation="MRLKKHYLCTALSLAFTQQAVAAQESDSLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLQGPNQMGGAINITTQKPTKPLEASLGYRQG
                     WSRSQDNAYDMHASFAASSELGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMINSSA
                     DDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNSGQKSRYWQWPEYDKESFYY
                     QGTTQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQLAAD
                     VRENDLLTFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVAGISY
                     DWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFVNEDTLALSYYDRKRFPTLK
                     ERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTHDWGFEVSVYYNRVSDAILSH
                     NIDADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKITDLPT
                     QTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHGFSVN
                     ASVNNLFDTQYAYSEGFVEEGRNFWAGVEYTF"
     gene            complement(6488..6742)
                     /locus_tag="ACHJWA_004417"
     CDS             complement(6488..6742)
                     /locus_tag="ACHJWA_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO4310879.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            6943..>7531
                     /locus_tag="ACHJWA_004418"
     CDS             6943..>7531
                     /locus_tag="ACHJWA_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFO4310880.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWIGGQLISKIL
                     VITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AGLHEGMRFAQIEVNMGQWGVFHFDAQLISISERKV"
BASE COUNT         1828 a         1997 c         1923 g         1783 t
ORIGIN      
        1 ggatataact tatgtcacta accagacgga ggtttacaca gattcttgcg tcgacgttgt
       61 tcctgcatca tctgccttct tttgcacaat cagtcaaatt ctgggcctca ctgacgcttc
      121 ccgaagccca aaacattaca cgcatcgtca gcgcaggtgc gcccgccgat ttgctattgc
      181 tggctgtcgc gccagaaaaa atggtcggtt tttcctcttt tgattttgcc cgtcaggcat
      241 taattccatt gtcagagcac attcgccagt tacccaggct gggacgactc gccgggcgcg
      301 ccagcacact ctcgctggaa gggcttatgg cgttacatcc cgatttggtc gttgattgcg
      361 gcaatacgga tgaaacctgg atctcccagg cacggcaggt tagcgaacag acacaaatac
      421 cctggttatt gcttaacggg aaactggcgc aatcagcaga acagttaaca acgcttggca
      481 aaacgttagg cgaagagcat cgcgccgcag aacaagccaa tctcgccagc cgcttcgttg
      541 gtgaagctca ggcattcgcc gcctcacccg ccgctaacct ccgcttttat gctgcgcgcg
      601 gccctcgagg gctggagacg ggcttaaagg gttcgttgca taccgaggcg gcggaattat
      661 taggtttgca caacgtcgcg caaatagccg atcgccaagg cctgacacag gtttccatgg
      721 aaaatctcct gcgctggcag ccggatatta ttctggttca ggaggccgtt actgcagatt
      781 ttattcgtcg tgatcctctc tggcatggcg tgaaagccgt tgcagaacaa cgcatcttat
      841 ttttaagtgg cttgcccttt ggctggctgg atgccccacc gggaatcaac cgtcttctgg
      901 gattacgcag acttcacgcc tggctggatc ccgccatcaa tcgccagttt aaaagtgaca
      961 tgcagcatta cgcccaactg ttctggcatt gttcactcag tgacgccgac tatcaaaaat
     1021 tggtggcgac ctaatgagaa tcgtaaacgg atgtattttg ctgctggcag ctatcagtat
     1081 cacttttgcc gccgtaagtg gggcttatca tctcgatatg cagcaactgc tggcgctaat
     1141 tcttcgtcaa gaaaatgttc ctgtgcagga acaaattgtc ttctggcaaa tccgcgtgcc
     1201 gcgtattctt gctgcgctgt tactgggcgc ggcgctggca ggagccggaa ccacctatca
     1261 gggaatgttg cgtaatccct tagtttcacc ggatatttta ggcgtctccg ccggtgccgg
     1321 acttggtgcc tgcacggcaa ttctgtgggg attatccatt gtttacatcc agctgtacgc
     1381 attttgtggc gggctgatgg tggtcgcggg cgtctggttg attacgcgtc gggtgacccg
     1441 ccacgatccg atcttaaccc tggtgctggt aggcatcgcg ttaggaacgt tgtgcggagc
     1501 ggggatatcg ctaattaaaa cgctggccga tccttacacc cagttaccgt caattacctt
     1561 ctggttgctg gggggactgt ccaccgtgac cttacgcgat ctctgctatg ctgcaccgat
     1621 cattctcata ggctccttgc ccctgttttt tctccgctgg cgcatgaatt tactcacgct
     1681 ttccgacgac gaagcccgtt cgctgggact gaacgtgacg cgtctgcgct ttgtattgat
     1741 tgtctgcgcc acactaatca ccgccagtac ggtagcaata gcaggcataa ttggttgggt
     1801 cggcctggtc gttccgcaca ttgcccgctt gttgaccggt cataaccatc agcagctgtt
     1861 gcccatggca atgtgtactg gcgcgattct gctcctgctt accgatacgc tggcacgcag
     1921 cattggaaca acagaaatcc cacttggcat tctgaccgct tttgttggcg caccgttttt
     1981 cctgcttttg ttgttacgcg gaggtcgcca gtgactttgc ttgccgtccg tcatgcgtct
     2041 ctgggttact cccgccatcc ggtgctacgc gacgtgtcct tcaccctgtc gcaaggaaca
     2101 atttgttgtc tgttaggagc caatggttgc ggcaaaacca cactcatgcg ctccattctt
     2161 ggcgttatac cgttattaaa aggcgaggtc ttactggata gcctccctgt gcagacactc
     2221 agccatcgcc agcgcgcgca ggctattgcc tgggtgccgc aggcgcacga cggtattttt
     2281 gcttttagtg tgctggatat ggtgttgatg ggcctagccc ccaccatagg cgctttttcc
     2341 gttcccggta agcaagaacg cctgaaagcc acggagcaac tggaaaagct gggcattttg
     2401 catctggcgg cacgacgctg gaatacgctt agcggcggtg aacgtcagct ggctttaatc
     2461 gcccgtgcac tcgtccagca accgcgcttg ttgttactgg atgagcctgc ctccagcctc
     2521 gatttcggtc atcagatcca actactggac acgctggcac agctcaaaaa taacggcatg
     2581 acaatgttaa tgtcgactca tcatccgctc catgccaacg ccattgccga cagcattatc
     2641 caggtagagc cagatggacg agtaacacaa ggcttaccga ccgaacagtt aacaacaaac
     2701 aaactggctg ccctgtatcg ggtttctgcc gaccagattc atcatcacct ctctgcaatt
     2761 agccactaag gaaaaatcat gctcattgat gatattgatt ttgcagacct ttatctgcag
     2821 caactcaagc tggcccaccg cacagaaaaa acgccggatc actgggatca acgggcggaa
     2881 aaaatggcgg aaaattgcgc ctcgcctacg gacagctacc tgcaacaact gatcgctaaa
     2941 attgacctac aaggcgctca gaccttattc gatatgggct gcggaccggg taccgtatcg
     3001 ctggcgctgg ctgacaaact cacgacgatt tatggcgttg attacagtca ggggatgctg
     3061 aacgttgccg cccgtcgcgc cgccgcgctg aaggcagaca atgtccactt gatccagcgc
     3121 gcctgggaag aggactggag tgacctgcca cgctgcgata ttgccgtggc ttcccgctcc
     3181 actctggtgg ctgatatgcg ccaggcaatg agcaaactga ataatcaggc acggcttcgc
     3241 gtctatacga ctcatctggt cagcacctcg ttcgtctctc cagccattca gcgggcggct
     3301 ggtcgggaag tgatcgaatt acccaactat atctttgcgc tcaacgtcct gtatcagatg
     3361 ggaatttatg cccacgttga ttttatccgt ggacaaaatt gccaacagga taacagcacc
     3421 taggaacgtt ttgagcagaa cgtcagctgg agtcttggcg cgcttaacga cgatgagcgt
     3481 gagcggttat atcgctggta tcaacaacaa gatgctcgtg ccctcgcccc tgccagccgc
     3541 gactgggcgc tcatctggtg ggatagcgta ccgcaggagg cactgcgatg attttccttt
     3601 ctcaggcaca aatcgatgcg ttacttctgg aagatatcca gggcggtgac ctgaccacga
     3661 gagcattaaa tattggacac cagcatggct atatagagtt ttttctccgt cagggcggct
     3721 gcgtcagcgg cgtttctgtc gcgtgtaaga tgttaactac gctgggatta actattgatg
     3781 acgcggtcag cgacggttca caagcgaacg cgggtcagcg gctaatccgt gcgcagggta
     3841 atgccgcagc acttcatcag ggatggaagg caatccagaa tgtgctggag tggagttgcg
     3901 gtgtttctga ttatctcgat caaatgctgg cgttacttcg tgaacgttat cctgatggca
     3961 atatcgcctg cacccgaaaa gcaattccgg gcacccgctt actggcctcg caggcaattc
     4021 tggctgccgg aggactgatt catcgtgccg gatgtgcgga aaccatatta ttgtttgcca
     4081 accaccgcca ttttcttcat gacaatcagg actggtcagg cgcaatcaat cagttacgtc
     4141 gccacgcacc cgagaagaaa attgttgttg aagccgacac gccgaaagag gcaatcgccg
     4201 cgttacgcgc gcaaccagac gtacttcagc tcgacaaatt tagtccgcaa caggcaactg
     4261 aaattgcgca aatagcgccg tcgctggctc cccactgcac gctggctctg accggcggaa
     4321 ttaatctgac gacactcaaa aattacctcg actgcggcat tcgcctcttt attacctccg
     4381 cgccttatta cgcggcaccg gctgacatta aagtcagtct gcaacccgca gcctctattt
     4441 aattctttgt taaacggaat ttattctatg agactcaaaa aacattatct ctgcacagca
     4501 ttatcacttg cctttaccca gcaggccgta gcggctcagg agagcgactc gctgaccgta
     4561 tggtccagtc cggtatcatc gacgacgacc accgttctcg atcaacccac catgaaggcc
     4621 ctggataaac agaatgtcgc tcaggcatta agtgtcgtcc ccggcgtggt gctgcaaaag
     4681 tcaggcagcc gcaacgaaga acaggtaaaa gttcgtggct ttgatagtcg gcaggtgccg
     4741 gtctatttcg acggtgtgcc catttatgtt ccctatgacg gcaacctcga tctggcgcgg
     4801 attctgacca acaatctggg ggcagtcgaa gtttccaaag ggtattcgtc gctgcttcag
     4861 ggacctaatc agatgggcgg agccattaat atcaccaccc agaagccaac aaaacctctg
     4921 gaagcaagtc tgggatatcg ccagggatgg agccgtagcc aggacaatgc ctatgatatg
     4981 catgcttcat ttgccgccag cagcgaactg gggtatttgc aagtcagcgg tagccagcta
     5041 aagcaggatt ttctcggcct gccgcatggt gtaaataatg atattgcagg caaacacggc
     5101 aagatgatta attcatcggc tgatgataaa cgcggcattg tgaagctcgg atttacacca
     5161 cgtgaaaacg atgaatacac actgacttac attaagcagg atggtgaaaa agataacccg
     5221 ccatacagcg gaaatagtgg tcaaaaatca cgctactggc agtggccaga gtatgacaaa
     5281 gaaagttttt attatcaggg aacgacccaa ctaaacgatc gttttaccct gaaaagtcgg
     5341 ctgtatcgcg acacctttga aaatacgctg atgatgtaca actcgctggc tgatttgaaa
     5401 aataaaaaag gcagctacag ccattattcc gattacagcg acggtgccgg attacaactg
     5461 gcagccgatg tgcgtgaaaa cgatctgctg acgtttgccg ttaactggaa agatgacgta
     5521 caccgggaaa aaggtgcgcc gcacgccgct tacgatcgct atgaagatcg tacctggtcg
     5581 ctcgccagtg aatatcaatg ggctgctgcc gataatgtcg atgttgtggc tggaatcagc
     5641 tatgactggc gcgatagcgt agaagcgaaa aaacacgaga aagatggcag catcacccat
     5701 tatgacgaca acaatcagtc agcttttaac tggcaggtga tggggaaata ccactttgtc
     5761 aatgaagaca cgctggcgct ttcgtactat gaccgcaaac gctttccgac gctgaaagaa
     5821 cgctatacca catccaaacc tgcgtataac cagatagcga ttgttaaccc gcagctcaag
     5881 ccggaacgcg ctcgcggggt ggatttaacc tggaatggtg ccttcacgca cgactgggga
     5941 tttgaggtca gcgtttacta taaccgggtg agtgatgcca tcctctcgca caatatcgat
     6001 gccgatacca ttcaaaatca gaacagcggc acggtggatt acagcggtct ggatgccggt
     6061 attaagggga aaatcagcaa tatactggat gtaggattga gctacgccct gatccacgct
     6121 gacgccaaac gtaaagacat cggcaagata accgatctgc caacgcagac aatgaccgca
     6181 tggatgactc tcaaaccgtg ggagccgtta agcgtaacgc tgtcggaaga ggcgcgttcc
     6241 tccagctaca gtaacagtga cggttcacaa aaagccgccg gttttgcggt gacccacatt
     6301 cgagccgatt acaccttagg tcatggcttc agcgtcaatg cgtcggtcaa taacctgttt
     6361 gatacccaat atgcctacag tgaagggttt gttgaagaag gtcgcaattt ctgggcaggt
     6421 gtcgagtata cgttctgatg tgaaagcgga tagcggcatc tgtatcgcta tccgccttat
     6481 gaaaatctta ttctcttcgc aggaggcgga atacgcccag aacgagaata gctcccacca
     6541 ccgccaccag gaagctgtgc aaattaaaac cgctgatgga gccgccaatg ccaaacatgg
     6601 tcgccagcca gccgccgacc accgcaccga ctatcccgag aatacaggtc aggaaaaatc
     6661 caccaccatc acgccccggc atgattagct tggcgataat gccggctatc aggccaaaaa
     6721 taatccaggc aataattccc atgttcaggc cctcgtttcg atgaatcgga cgcaagaaat
     6781 gcttacgtca acgctctaag tataggcaag ggcgggatcg atgctgggcg tatgtcggct
     6841 gatttaagaa caacatgtaa aaaatattca cgcatttgta ttaagttttg ttaactgtga
     6901 ccgataaacc aaagacagtt tgtcagtcag gagtttttcc gcgtgagtca ttaccatgag
     6961 cagttcctga aacaaaatcc gttagccgtc ctgggcgtgt tacgcgattt gcacaaagcc
     7021 gcaattcctt tgcgtctcag ttggattggc gggcagctga tcagcaaaat actggtaata
     7081 accccggata aactggtgct ggatttcggc agtcaagccg aagacaacat cgccgtgcta
     7141 aaggcacagc acattaccat taccgccgaa actcagggtg cgaaagtcga gtttactgtt
     7201 gaacaactac agcagagtga atacttgcag cttccggcat ttattaccgt accgcctccc
     7261 accttatggt ttgtacaacg acgccgatat ttccgcatct ccgccccact ccatccgcct
     7321 tatttttgcc agaccaaact ggcggataac agtacgttac gtttccgcct gtatgatttg
     7381 tcgttaggcg gcatgggcgc attactggaa acagcaaaac ctgccgggtt gcacgaaggt
     7441 atgcgcttcg ctcagattga agtcaacatg gggcaatggg gtgtttttca ctttgacgcc
     7501 cagttaatct ccatcagcga gcgcaaagtg a
//
LOCUS       DAZFAB010000164         7441 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZFAB010000164 DAZFAB010000000
VERSION     DAZFAB010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.164"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            464..697
                     /locus_tag="ACHJWA_004419"
     CDS             464..697
                     /locus_tag="ACHJWA_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310881.1"
                     /translation="MLKSTLIAKCLYQNRMVSSISIGESAVKSIFEEYFPGHDFNKWN
                     TKLPPAVSTRILKATERASTIRVNYFIKDLWDL"
     gene            complement(675..1082)
                     /locus_tag="ACHJWA_004420"
     CDS             complement(675..1082)
                     /locus_tag="ACHJWA_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310882.1"
                     /translation="MEFKDLPTPLQEMASNIVRSQLATLDLSTAEKETIDNMVRNVRN
                     AFSGLYGSDNQKQESDVNKRVISVCVNGHVLSSIKTETATVFDCLCIVQSLVDALFRS
                     VNLENDANLRGRIIAHPYAHTLGSVDIKDPTNL"
     gene            complement(1105..1323)
                     /gene="ydfC"
                     /locus_tag="ACHJWA_004421"
     CDS             complement(1105..1323)
                     /gene="ydfC"
                     /locus_tag="ACHJWA_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFO4310883.1"
                     /translation="MQKRDPVIIAPDYTDDELYEWMHQKIKAVQDLKWANEARAKQAE
                     NLSALEQDITNLEKAAALSIARMITYPR"
     gene            complement(1396..1695)
                     /locus_tag="ACHJWA_004422"
     CDS             complement(1396..1695)
                     /locus_tag="ACHJWA_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310884.1"
                     /translation="MALYIDISAIAGQVRVIRAVTKRYAPLLQKVSGECTEDIVNDFV
                     IELRGLIFSYKVTTIFADGSRETVRALRLKGCVKDLATTFWARKLDCIHNQFPLE"
     gene            complement(1959..2366)
                     /locus_tag="ACHJWA_004423"
     CDS             complement(1959..2366)
                     /locus_tag="ACHJWA_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4310885.1"
                     /translation="MKNETFGARLLYRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPVNLTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            2443..2670
                     /locus_tag="ACHJWA_004424"
     CDS             2443..2670
                     /locus_tag="ACHJWA_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO4310886.1"
                     /translation="MLKIDAIAFFGSKTKLANVAGVRLASVAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRAGRLNNENHH"
     gene            2654..3205
                     /locus_tag="ACHJWA_004425"
     CDS             2654..3205
                     /locus_tag="ACHJWA_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO4310887.1"
                     /translation="MKITTEQVCEALDTWVCRPGMTQEQATILITEAFWALKERPNID
                     VQRVTFNDGEVDQRALGVNRVKIFERWKAIDTRDKRDKFTALIPAIMEAIRISDFRLY
                     CEITDGKSITYMIAGLNKEYGDVVESGLLFADPVVVERETDELIEKAIAFKHAYRQQY
                     QYYFADKQMSARGAYEYRCTTMG"
     gene            3177..4217
                     /locus_tag="ACHJWA_004426"
     CDS             3177..4217
                     /locus_tag="ACHJWA_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFO4310888.1"
                     /translation="MSIDALRWAKKVKTGSSSSKSVLTWLADMCGADLCAYPSVSALA
                     EVTELNKKTVQDSLRHLMEIGLIVDTGERKGRTKQIVVYRLIGVEESVAEPEYTQKRE
                     SLKVGKIGAVNKNSTENGYVSAQNRPKNRTLSCMENNQRHPNFPSKTPKNGSRNPKEP
                     KDLNPTHNARESAPTSEQEVLSLQAAPPVFLYGLSEPIGKFPMSDSWYPSRDFRRRAA
                     LWGMALPETEFTPAELAAFRDYWAAEGKVFTQIQWEQKFARHVNHVRAQVKPVSKGVN
                     HAAAPGGTASRAVQEIRAAREQWERENGFISDGNGLEAVGTHGGGLFEPLDPEERGRT
                     FEALDCTDWRDD"
     gene            4210..4671
                     /locus_tag="ACHJWA_004427"
     CDS             4210..4671
                     /locus_tag="ACHJWA_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO4310889.1"
                     /translation="MTEQQIRQVCRQCMDRCRAGETWPPDLAEFVALISKSGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWSQPVLYHICLEMRSKGIERQMTEGELKRLAE
                     RQLTKWAKHVSNGLSVPPVRRQLAAPKRPSGPTPIELLKQEYERRKAAGFV"
     gene            4759..5439
                     /locus_tag="ACHJWA_004428"
     CDS             4759..5439
                     /locus_tag="ACHJWA_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO4310890.1"
                     /translation="MAARLDISREEVLNELWELKKAGFVDKSVYTWRVADNNVQQEQP
                     APEETTTATEAKISECDLTATIEQRGPQTADELATLFGTTSRKVASTLAMAISKGRLI
                     RVNQNGKIRYCIPGDNLPAEPKVESVAETDGKAFPQPAGVALPVQKDATQEDIKTETV
                     ADIVQSLPSFTATREDDLILPSLHMANRELRRAKNHVQKWERVCAALRELNKHRDMVA
                     GICRKSGQ"
     gene            5436..5600
                     /locus_tag="ACHJWA_004429"
     CDS             5436..5600
                     /locus_tag="ACHJWA_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310891.1"
                     /translation="MSGWCRPEIMILTMKVKCIGSLIGRSEAAVRMKAQVKGISLILR
                     GDFHQSTKYP"
     gene            5605..6138
                     /locus_tag="ACHJWA_004430"
                     /pseudo
     CDS             5605..6138
                     /locus_tag="ACHJWA_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498860.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            6299..7057
                     /gene="paa"
                     /locus_tag="ACHJWA_004431"
     CDS             6299..7057
                     /gene="paa"
                     /locus_tag="ACHJWA_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcfC family adhesin Paa"
                     /protein_id="HFO4310892.1"
                     /translation="MRNIMAGFLIFLSSAAYADINLYGPGGPHTALLDAAKLYAEKTG
                     IIVNVHYGPQNKWNEDAKKNADILFGASEQSALAIIRDHKDSFSEKDIQPLYLRKSIL
                     LVKKGNPKNIRSIDDLTRPGIGVIVNDGGGTSNTSGTGVWEDIAGRKGNIETVAAIRK
                     NIILYAPNSGTARKALENQPGADVWITWADWAASNPEIGDVVEIAPDYVIWRDMNITV
                     RQDANDETRRFAEWLQTDEAAPAFKKYGWTRKGT"
BASE COUNT         2057 a         1620 c         1905 g         1859 t
ORIGIN      
        1 cccataacac tgtttttagc tttaactgtt ccgcgtgcgc ttagccgcat tcactgcatc
       61 acaaaattca ctttaaaaag ggcggacatc agttcatggg caaacagatg ccgccaaacg
      121 tcaccagaaa attgataaca gagggcgttg cagcggggtt gtcacttaag cgtatggtca
      181 acctgacaac ccggtgtcct caacggggaa ggaataaccc cgccatactt accgctgcgc
      241 catttcgcgg agtgccacaa ccggaagcgc acggtcgacg aaaatttaac gacaggctat
      301 ctatgaacca gcaacctcgc cgtgcgcttt cgcgttatgc tctgactttt cagagaaata
      361 tcctttcagt aaactgtcag tgccggatgt tcacccgtgt ccggcgcacg cactccactt
      421 cacccgtgga gaactcctta attaccaacc ctcaggaggg tgaatgttaa aatcaactct
      481 tattgctaaa tgcctttatc aaaatcgcat ggtaagcagc atttcaatag gcgagtctgc
      541 agttaaaagt attttcgaag agtactttcc cgggcatgat tttaataaat ggaataccaa
      601 attaccgcca gcagtttcaa cgcgtattct gaaagcaacc gaaagagcaa gtacaattcg
      661 cgttaactat ttcattaaag atttgtggga tctttgatat ccacagagcc taaagtatgt
      721 gcatatggat gtgctattat gcgccctcgc agatttgcat cattttctaa attcactgaa
      781 cgaaacaggg catcaacaag gctctgtaca atgcaaaggc aatcgaagac tgtcgccgtt
      841 tctgttttga ttgatgaaag aacatggcca ttcacgcaaa cagaaattac ccgtttatta
      901 acatcgcttt cctgcttttg attatcagaa ccatatagcc cagaaaaagc attgcgcaca
      961 ttacgaacca tattatcgat ggtttctttt tctgcggtac taaggtcaag agtagccagt
     1021 tgtgaacgaa ctatattcga tgccatttcc tgtaatggcg ttggtaaatc tttaaattcc
     1081 attattagcc tcgttggtta gctattaacg tgggtatgta atcattctgg caatgcttaa
     1141 tgccgctgct ttttccagat tggtgatatc ctgctccaga gcggacagat tttcagcctg
     1201 cttagccctg gcttcattgg cccatttcag gtcctgcacc gccttaattt tctggtgcat
     1261 ccactcataa agttcatcat cggtatagtc tggcgcgatg atgacggggt ctcgtttctg
     1321 catgtcggct ccttgtggtt agcgttgcct gctttttaac cacgtcaggc gaggtggtat
     1381 cctctgaggg gtctgttact cgagaggaaa ttggttatga atacaatcaa gttttcttgc
     1441 ccagaatgtg gtggctaagt ctttgacaca tcctttaagc cgcagggctc tgacagtttc
     1501 gcgggagcca tctgcaaaaa ttgtggtcac cttgtaactg aagatgagtc ctcgcagttc
     1561 gatgacgaaa tcgttgacaa tatcttcggt gcactcacca gagactttct gaagtaaagg
     1621 cgcataccgc ttagttaccg ctctgataac tcttacctgt ccggcaatgg cgctgatatc
     1681 aatataaagc gccatcgctg tttctttgct gatccctgga cgccttccat tctgatgttt
     1741 gactcgccca ctgagaaatc ctctgcttcc ccttaacgcc gggtagcgga actgtttgct
     1801 gagaacaccg tgcggtgtct tgatgagtag aatttagaat agcctaagag ttatggtcaa
     1861 gctttttgtg tagaaaaacc taagcttctt gatgtaaaaa acacaagtat ttgaaagttt
     1921 gtgcttttta ttacagagag ttgcgaaaaa aaggggggtt atttatttgc gcttcttttg
     1981 cgagctttga gtagttcttc aaaaagtttg ttgaaattct caactcgagc acgcatctct
     2041 gacaacagag ccttttgctc tgactcaggc agtgcgtcga acagttgaag caactctttt
     2101 tgatcttctg tcagattgac tggctgatta tctgggatcg gttcgcctgg ttgcttatct
     2161 tcatctccaa aaagaagcca agtcggcgag cactgaagcg cctggctcag tgcgaataat
     2221 ctcttccccg ctggctgtgt ttcatctctt tcccattgag aaattgttac gtgagccact
     2281 ttgaccagct tacctaatgc ggcctgagac agttttaatt tttttcgcct gtataagagg
     2341 cgagcaccga aggtttcgtt tttcatatta ggtaattcta atttttcttg acttaggttt
     2401 ctctacgatc tagtttcctt aggaaaatct aagggtttcg atatgttgaa aattgatgct
     2461 atagcgtttt ttggcagcaa aacaaagctt gccaatgtcg caggagttag gctggcaagc
     2521 gttgctgcat ggggggaact ggttcctgaa ggtcgcgcga tgcgcctgca agaggcatcc
     2581 ggcggggaac ttcagtacga ccccaaagtt tatgacgaat atcgtaaggc aaagcgggcg
     2641 gggcggttga acaatgaaaa tcaccactga acaggtttgt gaggctctgg atacctgggt
     2701 atgccgacca ggaatgacac aggagcaggc gacgatatta atcacggaag cattctgggc
     2761 tctgaaagaa cgcccgaaca tcgatgttca acgcgtcacg tttaatgatg gcgaggttga
     2821 tcaacgggcg ctgggcgtta accgggtgaa gatattcgaa cgctggaaag ctatcgacac
     2881 cagagataag cgtgacaaat tcacggcgct gattccggca attatggagg ctatccggat
     2941 cagcgatttc agattgtatt gtgaaattac tgacggaaaa agcattacgt acatgatcgc
     3001 cgggttaaac aaagaatatg gcgatgtggt ggagtccggg ctgctttttg cggatccagt
     3061 tgttgtggaa cgtgagactg acgagcttat agaaaaagct attgctttca agcacgcgta
     3121 tcgtcagcaa taccaatatt actttgcaga taaacaaatg tctgccaggg gggcgtatga
     3181 gtatcgatgc actacgatgg gctaaaaagg tgaaaaccgg cagttcatcc agtaagtcag
     3241 tattgacctg gcttgctgat atgtgcggtg ccgatttgtg tgcatacccg tctgtatctg
     3301 cactggcaga agtaacggaa ctgaacaaaa agactgtgca ggacagctta cgacacctga
     3361 tggagattgg gttaattgtt gataccggtg agagaaaagg cagaacaaag caaattgtgg
     3421 tgtaccgact tatcggtgta gaagaaagtg ttgccgagcc tgaatacacc caaaaacggg
     3481 agtctttaaa ggtgggtaaa attggtgctg ttaataaaaa cagtaccgaa aatggttatg
     3541 tttcagcaca aaacagaccc aaaaacagaa ctcttagctg catggaaaat aaccaaagac
     3601 acccaaattt tccatcaaag acacccaaaa acggatcacg gaacccaaag gaacccaaag
     3661 atctaaaccc cacacataac gcacgcgaga gtgctccgac cagtgagcag gaagttttgt
     3721 cgttacaggc agccccccct gtattcctgt atggcctgag cgaacccatc ggaaaattcc
     3781 cgatgagcga tagctggtat ccgtcacggg attttcgacg acgggctgcg ttgtggggga
     3841 tggctttgcc ggagacagaa tttacacctg ctgaacttgc cgccttccgg gactactggg
     3901 cagcggaggg gaaagtgttt acgcagattc agtgggagca gaaattcgcc cgtcacgtaa
     3961 atcacgtcag ggcgcaggtt aaaccagtca gcaaaggggt aaaccatgca gcagcaccag
     4021 gtggcaccgc atcacgggca gttcaggaaa ttcgggcagc acgtgagcag tgggaacgtg
     4081 aaaacggatt tatcagcgac ggaaacggcc tggaagctgt gggaactcat gggggtggtt
     4141 tattcgaacc gctggatcca gaagaacggg gccgcacctt cgaagctctg gattgcacag
     4201 attggcgcga tgactgagca gcaaatccgg caggtctgcc gccagtgcat ggaccgctgc
     4261 cgggcgggtg aaacatggcc tccggacctg gctgagtttg tggcactgat ttcgaaaagc
     4321 ggagccaatc cattcggtct gacggtggat gctgtgatgg aggagtaccg ccgctggcgc
     4381 aatgagtcct ggcgatacga cggaagtgat aagtacccgt ggtctcagcc tgtgctgtat
     4441 cacatttgcc tcgagatgcg ttcaaagggg attgagcgcc agatgaccga aggggaatta
     4501 aaacggcttg cagaacggca actgacgaaa tgggcaaagc atgttagtaa cggcctgagc
     4561 gttccgccag tccggcgaca actggcggcc cccaaacgcc cgtcggggcc aacgccaatt
     4621 gagttgctga aacaggaata tgaacgccgg aaagcggctg gttttgtttg agttgagaag
     4681 tgatttttta ccgggaggaa atttatggag actgtttttg acgcactgaa agcgatggga
     4741 aaagccacgt cggtatagct ggctgcgcga cttgatatca gtcgtgaaga agtactgaac
     4801 gagctgtggg aactgaaaaa ggctggcttc gttgataaaa gcgtatacac ctggcgtgtg
     4861 gctgataaca acgttcagca ggaacagcca gcgccagaag aaaccaccac ggcaacagaa
     4921 gcgaaaatct cagagtgcga tttaaccgcg acgattgaac aacgcggacc acaaacggcg
     4981 gatgaactgg ctacgctgtt cggtacaaca tcccgcaaag ttgcttcaac gctggcaatg
     5041 gcaatcagca aaggtcgtct gattcgcgta aaccagaacg gtaaaattcg ttactgcata
     5101 ccaggtgata atttaccagc agagccgaaa gttgaatcgg tagcggaaac cgatggtaaa
     5161 gcctttcctc agccagccgg tgttgcgtta ccggtacaga aagatgcaac acaggaagat
     5221 attaaaacag aaactgtggc ggacattgtg cagtcgctgc catcgtttac tgcaacgcga
     5281 gaagatgatt tgattttgcc atcgctgcat atggcaaatc gcgaactgcg tagggcgaag
     5341 aatcatgtcc agaagtggga gcgagtctgc gccgcgctgc gggagctgaa caagcaccgg
     5401 gatatggttg ccgggatttg tcggaagtcc gggcaatgag cggatggtgc aggcctgaaa
     5461 tcatgatact aacaatgaag gtaaaatgca tcggcagtct gattggtcgt agtgaggcgg
     5521 cggtcaggat gaaagcccag gttaagggaa taagcctgat tctgcggggt gattttcacc
     5581 agtcaacaaa atatccgtag cgcgataacg gtcaaaaatt atggcgctga cacttttgtg
     5641 ccactggaga tgactgtacc taagttcagg ggagaagaac acgtccggtg ggatggtcgg
     5701 gccagattta aagggcaggt catggctcca gcctgtacgc tggcaatgga ggctgcctgg
     5761 cgggaaattg atatgggaac cacgccactc agggatttac tgccggtcca gagaataaat
     5821 tcctgttacg gttacaccac tgtgatcttg caagtgcagg aaagtaggtc tacacggtaa
     5881 cgcgagtgcg tgtaactttt gatgtcattc ccgtagaaac accggacaaa ttttcgctga
     5941 caggtcatgc agaaggtata aatctgcaga ttatggacaa ttacggatat ccggcaagag
     6001 ccggaaaaag catgccgcct ctaattctca gtggaagatg gacttgatta tactcattgc
     6061 attgtcagaa atagttatcc attaaaggct ggctattcca aacaggatgt tgattacaaa
     6121 aatgtaatca acatgtaagg tttatactct tcaatatgcg tataattttc cttattttgt
     6181 tgactttaaa taacaagcta tgcacgaggt aaagtcggat aagtttatct ggatgtaata
     6241 tatattattt gtagtgttta taactttatt tcatgataac caataaaagg agttttttat
     6301 gaggaacata atggcaggtt ttttaatatt cctgtcttct gctgcttatg ctgatatcaa
     6361 tctgtatggt cctggtggcc cgcatacagc cttgcttgat gcagccaaac tttatgccga
     6421 aaaaacaggt attatagtga acgttcatta cggcccacag aacaaatgga atgaagatgc
     6481 caaaaaaaat gcagatatct tgtttggcgc atcagaacaa tctgctctgg ctatcattcg
     6541 ggaccataaa gacagcttca gtgaaaaaga tattcagcct ctttatctgc gaaaaagtat
     6601 tttactggta aagaaaggta atcctaaaaa tatccggagt attgacgacc tgaccagacc
     6661 tgggattggc gtaattgtta atgatggtgg tggtaccagt aatacatcag gcactggcgt
     6721 ctgggaagat attgccggac gtaaagggaa tatagaaact gtcgccgcaa tccgaaaaaa
     6781 tattatttta tatgcgccca atagcggaac tgcacgtaag gctcttgaga atcagcctgg
     6841 agcagatgtc tggataacct gggctgactg ggcagccagt aatccagaaa ttggtgatgt
     6901 cgtggaaata gcgccagact acgtgatatg gcgtgatatg aacattacag tacgtcagga
     6961 tgcaaatgat gaaacccgtc gatttgcaga atggctacaa accgatgaag cggcgcctgc
     7021 attcaaaaaa tatggctgga ccaggaaagg cacttgacat cctcgtcctt caggacgtgg
     7081 attctttttc cggatgccgc gccagcggca tgtaggggca gctcacaaaa cggaaaaaat
     7141 tgtacgctaa gcctcgccag gtgaactgaa ttcattccga tatgggaatt cccatatcgg
     7201 gcgaaaacgg tttgctgtaa cggcagagtt aagtaggatt gctgcgggtg cttgaggcta
     7261 tctgcctcgg gcatgaacac caacggcaga tagataaaag ccccacccga ctataaatcg
     7321 aagtgaggcc cctatatgct cgtcacatat agattgcctc ttacggaccg aaaggtcaag
     7381 gagaagcagg ctatgaagca gcaaaaggcg atgttaatcg ccctgatcgt catctgttta
     7441 a
//
LOCUS       DAZFAB010000165         7422 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZFAB010000165 DAZFAB010000000
VERSION     DAZFAB010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7422
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.165"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(198..404)
                     /locus_tag="ACHJWA_004432"
     CDS             complement(198..404)
                     /locus_tag="ACHJWA_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4310893.1"
                     /translation="MNQHNTRLIRLPEVLHRTGYGKAWIYHLINQNRFPSPVKIGARS
                     VAFIESEVDEWIQLTIENSRKHVA"
     gene            complement(504..1355)
                     /locus_tag="ACHJWA_004433"
     CDS             complement(504..1355)
                     /locus_tag="ACHJWA_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6387 family protein"
                     /protein_id="HFO4310894.1"
                     /translation="MKNWSVEHTREVKKWLDIDTYRGFEELPLIHLYHELLARTLFFK
                     PYHEEFEAEAVRLYIDRIFTGKPFLITEKHLGYLTREDTLYQPPHFFLTTTERLAQLS
                     IVGLRNSLFFWDGSDEYSVNREFLDSPVSEVLPKLFRDTVMVEIDLANGTDEEIAESL
                     KAALPQWRKVRGIEPDVTEAIRFGYGTIKKIINYRLLPMIDILVWSKLYKVRISEDRL
                     SRLLYTDDDDEINTRLNHQIRDTDKPLALKAASIPFIRQFNLFINKNSHLKKIRVSDV
                     MKIADSD"
     gene            complement(1782..6368)
                     /gene="ypjA"
                     /locus_tag="ACHJWA_004434"
     CDS             complement(1782..6368)
                     /gene="ypjA"
                     /locus_tag="ACHJWA_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin-like autotransporter YpjA/EhaD"
                     /protein_id="HFO4310895.1"
                     /translation="MNRTSPYYCRRSVLSLLISALIYAPPGMAAFTPDVIGVVNDETV
                     DGSQRVDERGTTNNTHIINHGQQNVYGGVSNGSLIESGGYQDVGRHNNYVGQSNNTTI
                     NGGRQSIHDGGISTGTIIESGNQDVYKGGISNGTTIKGGASRVEGGSANGTLIDGGSQ
                     IVKVQGHADGTTINKSGSQDVVQGSLATNTTINGGRQYVEQSTVETTTIKNGGEQRVY
                     ESRALDTTIEGGTQSLNSKSTAKNTQIYSGGTQIIDNTSSSDVIEVYSGGVLDVSGGT
                     ATNVTQHDGAILKTNTNGTTVSGTNSEGAFSIHNHVADNVLLENGGHLDINAYGSANK
                     TIIKDKGTMSVLTNAKADATRIDNGGVMDVAGNATNTIINGGTQNINNYGIATGTNIN
                     SGTQNIKSGGKADTTIISSGSRQVVEKDGTAIGSNISAGGSLIVYTGGIAHGVNQETG
                     SALVANTGAGTDIEGYNKLSHFTITGGEANYVVLENTGELTVVAKTSAKNTTIDAGGK
                     LIVQKEAKTDSTRLNNGGVLEVQDGGEAKHVEQQSGGALIASTTSGTLIEGTNSYGDA
                     FYIRNSEAKNVVLENAGSLTVVTGSRAVDTIINANGKMDVYGKDVGTVLNSAGTQTIY
                     ASATSDKANIKGGKQTVYGLATEANIESGEQIVDGGSTEKTHINGGTQTVQNYGKAIN
                     TDIVSGLQQIMANGTAEGSIINGGSQIVNEGGLAENSVLNDGGTLDVREKGSATGIQQ
                     SSQGALVATTRATRVTGTRADGVAFSIEQGAANNILLANGGVLTVESDTSSDKTQVNT
                     GGREIVKTKATATGTTLTGGEQIVEGVANETTINDGGIQTVSANGEAIKTTINEGGTL
                     TVNDNGKATDIVQNSGAALQTSTANGIEISGTHQYGTFSISGNLATNMLLENGGNLLV
                     LAGTEARDSTVGKGGAMQNQGQDSATKVNSGGQYTLGRSKDEFQALARAEDLQVAGGT
                     AIVYAGTLADASVSGATGSLSLMTPRDNVTPVKLEGAIRITDSATLTIGNGVDTTLAD
                     LTAASRGSVWLNSNNSCAGTSNCEYRVNSLLLNDGNVYLSAQTAAPATTNGIYNTLTT
                     NELSGSGNFYLHTNVAGSRGDQLVVNNNATGNFKIFVQDTGVSPQSDDAMTLVKTGGG
                     DASFSLGNTGGFVDLGTYEYVLKSDGNSNWNLTNDVKPNPDPNPNPNPNPKPDPKPDP
                     KPDPKPDPTPEPTPTPVPEKRITPSTAAVLNMAATLPLVFDAELNSIRERLNIMKASP
                     HNNNVWGATYNTRNNVTTDAGAGFEQTLTGMTVGIDSPNDIPEGIATLGAFMGYSHSH
                     IGFDRGGHGSVGSYSLGGYASWEHESGFYLDGVVKLNRFESNVAGKMSSGGAANGSYH
                     SNGLGGHIETGMRFTDGNWNLTPYASLTGFTADNPEYHLSNGMESKSVDTRSIYRELG
                     ATLSYNMRLGNGMEIEPWLKAAVRKEFVDDNRVKVNNDGNFVNDLSGRRGIYQAGIKA
                     SFSSTLSGHLGVGYSHGAGVESPWNAVAGVNWSF"
     gene            complement(6433..6552)
                     /locus_tag="ACHJWA_004435"
     CDS             complement(6433..6552)
                     /locus_tag="ACHJWA_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFO4310896.1"
                     /translation="MPHSYAEYHHFIMMSLMSGEHNTLALSLQDESPGLVPVG"
     gene            complement(6776..6934)
                     /locus_tag="ACHJWA_004436"
                     /pseudo
     CDS             complement(6776..6934)
                     /locus_tag="ACHJWA_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(7304..>7422)
                     /locus_tag="ACHJWA_004437"
     CDS             complement(7304..>7422)
                     /locus_tag="ACHJWA_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310897.1"
                     /translation="ITGVAPFDDILFMDDDFDDNSPEDAPIENSPVVNSPLV"
BASE COUNT         1852 a         1848 c         1595 g         2127 t
ORIGIN      
        1 ttatttgacg tcaaataaac aggtacggct agtgggctgt atccataccc gatattaaga
       61 aaaccatctc taagccttat agcaaaagct ctgccagaaa aaacgtaaag aattacgtta
      121 aatatagatt tttttaagat ttaaacttta caaatctgag aattaatcct cataaataag
      181 aagactgtca ttttatttca ggcaacatgc tttcttgaat tttcgatagt taattgaatc
      241 cattcgtcta cttcactttc aatgaaagca acagagcgcg caccgatttt aacgggggaa
      301 ggaaaacgat tttggttgat gaggtgatag atccaagctt taccgtatcc agttcgatgc
      361 agaacctctg gtaaacgaat aaggcgggtg ttgtgctgat tcatagtcgt ttcctctgtg
      421 ccgttttgat ggaaatgact atacaaatga atcagcagga aaaagaccga agctgggaac
      481 gccagagcta atggaggttg gtcttagtca gaatcagcga ttttcatcac gtctgaaact
      541 ctgatctttt tcaaatggct gttcttgttg atgaacagat taaactggcg aataaaaggt
      601 atactcgccg ccttcaaagc aagaggtttg tctgtatccc tgatttggtg gttcaggcga
      661 gtgttgatct catcatcgtc atctgtatac aacaggcgag aaaggcggtc ttctgatata
      721 cgtactttat ataattttga ccataccaga atgtcgatca tgggaaggag tctgtaatta
      781 atgattttct taatcgttcc ataaccaaag cgaatagctt ccgttacatc tggttctatg
      841 ccacgcactt tacgccattg aggaagtgca gcctttaggg attcagcgat ctcttcatct
      901 gtcccgtttg ctaggtcgat ttcgaccatg acagtatccc tgaacaattt tggcaaaact
      961 tccgatacag ggctatcgag gaattctctg ttaacagaat actcatcgct tccatcccaa
     1021 aaaaacagtg agttccgtaa tccaactatg ctgagttgag ccagtctttc ggttgtcgtc
     1081 aaaaagaagt gcgggggctg gtaaagagta tcctctcttg tgagatagcc gagatgcttt
     1141 tctgtgatga gaaagggctt tccagtgaag atcctgtcga tgtagagcct tacggcttct
     1201 gcttcaaatt cttcatgata tggtttgaag aacaatgtcc ttgccagcaa ttcatggtag
     1261 aggtgtataa gcggaagctc ttcgaatccg cgatatgtat ctatatccag ccacttcttc
     1321 acctctctcg tatgttcaac tgaccagttt ttcataacga tctccaacag gcattagact
     1381 taatgtatgt agcaaacgta atcgacatgt taaaggaaga ccaatgaaat gagttcagat
     1441 gccatcagga agttgaacca aaaggtttga tattttttga tacagttcgc taacggacta
     1501 ttttcagggg tttatgccgt tacgatttat ggttccttta tagaaccaaa ggtaaagtca
     1561 gaaacagcag ttatctagaa aattcaatca aatagatatc tagttacagg gacgccagcc
     1621 aatcatgatt ggacggtgta aggacaacac caacaaaaac aggaagttag aagtctcagc
     1681 aggacaccga ccagacggtg aggagacata aaaggatacg caaaggagcc gcggctcctg
     1741 gtgacatgaa agcccacaga tgtgggcttt ttcgttgatg gtcagaacga ccagttcaca
     1801 ccagctaccg cgttccacgg ggattccaca ccggcaccat ggctataccc caccccaaga
     1861 tgcccgctta acgtactgct gaatgaggct ttaatacctg cctggtatat tccacgtctg
     1921 cccgacaaat cattgacgaa attaccgtca ttattcacct tcacccggtt atcatcgaca
     1981 aattctttgc gcacagccgc cttcagccac ggctcaattt ccataccgtt ccccagacgc
     2041 atgttgtaac tcagcgttgc gcccagttca cgatatatac tgcgggtatc gactgatttc
     2101 gattccatgc cattggataa atgatattcg gggttatcag cggtgaaccc cgttaacgat
     2161 gcatacggcg tcaggttcca gttaccatcg gtaaatcgca tcccggtttc aatgtgaccg
     2221 cccagcccgt tgctgtggta actgccattg gcggctccac cgctgctcat tttaccggct
     2281 acgttacttt caaaacggtt cagcttcacg acaccgtcca gatagaaacc actttcatgt
     2341 tcccaactgg catagccgcc cagagaataa ctgcccacac tgccatgtcc tccgcgatca
     2401 aaaccgatat gtgaatggga ataacccata aaagcgccca gcgtcgcaat cccctcagga
     2461 atatcattag ggctgtcgat ccccactgtc attccggtca gcgtctgctc aaacccggcc
     2521 cccgcatcgg tggtgacatt attacgggtg ttatacgtcg ccccccagac attattgttg
     2581 tgtggactcg ctttcattat gttcaaccgc tcgcgaatac tgtttagctc agcatcaaat
     2641 accaacggta atgttgctgc catattgagt acggctgcgg tagaaggcgt gatgcgtttc
     2701 tccggaacgg gtgtcggcgt tggctcggga gtcgggtctg gtttcggatc cggttttggg
     2761 tctggttttg gatccggctt cggatttggg ttgggatttg ggttgggatc cgggttgggt
     2821 ttgacatcat tggtcaggtt ccagttgctg ttgccatcgc ttttcaggac atactcatag
     2881 gtcccaagat caacgaaacc gccagtattg cccagcgaaa acgaagcatc ccctccccct
     2941 gttttcacca gcgtcatcgc gtcgtcagac tgaggactga cgccggtatc ctgaacaaag
     3001 attttaaaat taccagtggc gttgttgttg acgaccagtt gatcgccccg agagcctgca
     3061 acgttggtat gcaggtagaa attaccgcta ccggaaagtt cattggttgt cagcgtattg
     3121 tatataccgt tagttgtggc aggcgctgct gtttgtgctg ataaataaac attaccgtcg
     3181 ttaagtagca aactgtttac tctatactcg cagttgctgg tgcctgcaca ggaattattg
     3241 ctgttaagcc agacactgcc ccggctggca gccgtcaggt cggcaagcgt cgtatcaacg
     3301 ccattgccga tagttaatgt cgcgctatcg gtaatccgga tcgccccttc gagtttaact
     3361 ggcgtaacat tatcccgtgg cgtcattaac gacaggcttc ctgtcgcgcc actgaccgat
     3421 gcatccgcca gcgtacctgc gtagacgatt gctgtcccgc cagcaacctg gagatcttct
     3481 gcccgggcca gagcctgaaa ctcatctttt gaccgcccaa gggtatattg cccaccagag
     3541 ttaacctttg tggcggagtc ctgaccctgg ttttgcattg cccccccctt gccaaccgtg
     3601 gagtcgcgag cttcggtacc tgctaatacc aataaattac cgccattttc cagcaacata
     3661 ttggtcgcta aattgccgga aatggaaaaa gtgccgtact ggtgagtacc gctgatttca
     3721 ataccgttag ccgtgctcgt ctggagagcg gcaccgctgt tctggacgat atctgtcgct
     3781 ttgccattat cgttgactgt cagcgtaccg ccttcattga tcgttgtttt tattgcctct
     3841 ccgttagctg aaactgtttg tattccgccg tcgttaattg ttgtctcatt cgccacaccc
     3901 tcgacaattt gttcaccgcc ggtgagcgtc gtgcctgtcg cagtggcttt tgttttgacg
     3961 atctcccgtc cgcccgtatt gacctgtgtt ttgtcagaag aggtgtctga ctccacggtt
     4021 aacactccgc catttgccag caggatattg ttcgccgcac cctgctcgat gctgaacgcg
     4081 acgccatccg cgcgtgttcc tgtgacccgc gtcgccctgg tggttgcaac caacgcgccc
     4141 tggctactct gctgtatccc cgttgcgctg cctttctccc gcacatcgag tgtgccgcca
     4201 tcattaagca ccgagttttc agccagaccg ccctcattaa ctatctgtga accgccatta
     4261 ataatggaac cttccgctgt cccgtttgcc ataatttgtt gtaggccaga gacgatatcg
     4321 gtattgatcg ccttaccata attctgaacg gtttgcgtgc caccattgat gtgtgttttc
     4381 tctgttgacc caccatcaac aatttgttca ccactttcga tatttgcttc agtggctaaa
     4441 ccatataccg tttgcttgcc acctttgata tttgctttat cagaagtggc actggcatat
     4501 attgtttggg tgccagcact attgagtaca gtgccaacat cttttccata aacatccatt
     4561 ttgccgttgg cattaataat cgtgtcaact gcccgggaac cagtgacgac tgttaatgag
     4621 ccagcgtttt ccagcactac atttttagct tctgaattcc tgatgtagaa agcatcacca
     4681 taactgttgg ttccttcgat aagtgttccg gaggtcgtgg aagcaattaa tgcgccgccg
     4741 gattgttgct caacatgctt agcctcacca ccgtcctgaa cctccagaac gccgccatta
     4801 ttaagtctgg tgctatctgt tttagcctcc ttctggacaa tcagcttacc gccagcatca
     4861 atggtagtat ttttcgccga ggttttagcc actaccgtca gttcgccggt attttccagc
     4921 acaacataat tagcctcccc tccggtaata gtgaagtgag agagcttgtt gtatccttcg
     4981 atatcagtcc ctgcacccgt gttggcaact aaagcactgc ccgtctcctg gttaacccca
     5041 tgtgcaatac cgccggtata gacaatcagc gagcctccgg cgctaatatt gctgccaatt
     5101 gccgtaccat ctttctcaac aacctgccgg ctcccggagg atataattgt tgtgtcagct
     5161 ttcccgccgc ttttgatatt ttgcgttccg ctgttgatat tggtgcctgt ggctatgcca
     5221 taattattaa tattctgtgt gccaccatta attatggtat ttgtcgcgtt tcctgcaaca
     5281 tccataaccc cgccattatc tattcgggtc gcatcagctt tagcattggt taaaactgac
     5341 attgttcctt tatctttaat aatcgtcttg tttgccgaac catatgcgtt tatgtctaaa
     5401 tgaccaccgt tttccagcaa cacattgtct gccacgtgat tgtggatgga gaatgcacct
     5461 tcactattcg taccgctcac cgtcgtaccg ttagtgttag tttttaaaat tgcaccatcg
     5521 tgctgggtaa catttgttgc cgtaccacca ctaacatcaa gcacgccacc ggaataaact
     5581 tcaataacat ccgaggagct ggtgttatca ataatttgcg taccaccaga atagatctga
     5641 gtattttttg ccgttgactt actattcaga gactgagttc cgccttcaat cgtcgtgtcc
     5701 agcgcacggc tctcatatac tctttgctca ccgccatttt tgatggtggt tgtttctact
     5761 gtgctctgtt caacatactg tcgaccacca tttatggttg tgttcgttgc cagacttcct
     5821 tgtactacgt cctgagagcc agacttattt atcgttgtac catcagcatg cccttgaact
     5881 tttactatct ggctaccacc atcaatgagt gttccattcg cactccctcc ctctacgcgt
     5941 gaagcaccgc ccttaattgt cgttccattg ctgatacccc ctttataaac gtcctgattg
     6001 ccactctcga ttatcgtacc tgtggaaata cccccgtcat gaattgactg tctgcccccg
     6061 ttaatggtgg tattattaga ctgccccaca taattgttat gccttcctac atcttgatat
     6121 ccaccagatt caataagact tccattagat accccgccat aaacattctg ctggccatgg
     6181 ttgataatat gagtgttatt tgttgtacct cgttcatcta ctcgttggct gccatctaca
     6241 gtctcatcgt ttaccacacc aataacatca ggagtgaagg cagccatccc gggcggggca
     6301 tatatcaagg cagatatcaa taaggaaagt actgagcgac gacaataata gggactggtc
     6361 ctgttcataa atttcatcct ctgaaaagtg aatactgagt agcgtttaag cgaccttagc
     6421 tttgctgcaa catcagccca caggcaccag accaggggat tcatcctgaa gagacagcgc
     6481 aagtgtattg tgttcaccgc tcatcaaaga catcatgatg aaatgatgat attccgcata
     6541 agaatgaggc attttttaaa cgcagtgcgc tgaagtgtgg ttggataaaa aagtcaatcc
     6601 attcaggaaa tacgggcgta ttcttttctt tcgacaatga ggccgttggc aaaataaaat
     6661 gatttacata atcgtttctg atgaatatct tctgctcaca taaaaatcac acaataactt
     6721 tgagatcgca gattgtttta cttttacagc attcgtcccc ccattgttgg gcaaatatag
     6781 attgggccag agcacgaaag ttaataccac gttcgcacag ctcctccaac agcacgacaa
     6841 gatgccacat actgcgcccc agtcggttca gtttacagac tagcagtgct ttccctgccg
     6901 atgatgtcct gagcagtttt ttcagtccgg acctcctgcc acggttttac gttgaagcgg
     6961 taaccctgag cacgcagttc ttcagtcagg cgtggtgcac cgtaacgctg ttattgctgg
     7021 gtgagatcaa aaaactttca ggcagctaag gaaagttgaa ccagacaata gatgaaatat
     7081 ttcaaccaat tacagcacca attcagtcac tgccagccca ccaaataaat caaggggtta
     7141 catgaaaacg tagccccttt ttctttggta gtgacactaa aatggatgta gtgtgaagaa
     7201 taatcccgtt tactcaatca ataatacata ttgtttcaat ctacgttatt atctctttat
     7261 aaaaattgcc atttatttat cattgaaaac tgcttttaga actttataca agaggactat
     7321 tcacaacagg actattctca atgggagcat cctcagggga gttatcatca aagtcatcat
     7381 ccataaataa aatatcatcg aatggtgcca cacccgtgat ca
//
LOCUS       DAZFAB010000166         7357 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZFAB010000166 DAZFAB010000000
VERSION     DAZFAB010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7357
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.166"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..872
                     /locus_tag="ACHJWA_004438"
     CDS             <1..872
                     /locus_tag="ACHJWA_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO4310898.1"
                     /translation="DSDIARLLLKLRKSRNLTVTELAQRSGVSQAMISKVERGTTDAG
                     AGFEQTLTGMTVGIDSRNDIPEGIATLGAFMGYSHSHIGFDRGGHGSVDSYSLGGYAS
                     WEHESGFYLDGVVKLNRFESNVAGKMSSGGAANGSYHSNGLGGHIETGMRFTDGNWNL
                     TPYASLTGFTADNPEYHLSNGMESKSVDTRSIYRELGATLSYNMRLGNGMEVEPWLKA
                     AVRKEFVDDNRVKVNSDGNFVNDLSGRRGIYQAGIKASFSSTLSGHLGVGYSNGAGME
                     SPWNAVAGVNWSF"
     gene            complement(1244..1510)
                     /gene="minE"
                     /locus_tag="ACHJWA_004439"
     CDS             complement(1244..1510)
                     /gene="minE"
                     /locus_tag="ACHJWA_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFO4310899.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(1514..2326)
                     /gene="minD"
                     /locus_tag="ACHJWA_004440"
     CDS             complement(1514..2326)
                     /gene="minD"
                     /locus_tag="ACHJWA_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFO4310900.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(2350..3045)
                     /gene="minC"
                     /locus_tag="ACHJWA_004441"
     CDS             complement(2350..3045)
                     /gene="minC"
                     /locus_tag="ACHJWA_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFO4310901.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            3565..3933
                     /locus_tag="ACHJWA_004442"
     CDS             3565..3933
                     /locus_tag="ACHJWA_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFO4310902.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLSEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(4036..4437)
                     /gene="pliG"
                     /locus_tag="ACHJWA_004443"
     CDS             complement(4036..4437)
                     /gene="pliG"
                     /locus_tag="ACHJWA_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFO4310903.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            4508..4666
                     /locus_tag="ACHJWA_004444"
     CDS             4508..4666
                     /locus_tag="ACHJWA_004444"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310904.1"
                     /translation="MAFLRSVKSRRGIFAGGITYKGAWTQQTVEIYSWLACGAGFIDV
                     SLCQNLAS"
     gene            4678..4971
                     /locus_tag="ACHJWA_004445"
     CDS             4678..4971
                     /locus_tag="ACHJWA_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFO4310905.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKQALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            5043..5702
                     /locus_tag="ACHJWA_004446"
     CDS             5043..5702
                     /locus_tag="ACHJWA_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFO4310906.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            5794..6240
                     /locus_tag="ACHJWA_004447"
     CDS             5794..6240
                     /locus_tag="ACHJWA_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFO4310907.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(6447..7357)
                     /gene="hlyE"
                     /locus_tag="ACHJWA_004448"
                     /pseudo
     CDS             complement(6447..7357)
                     /gene="hlyE"
                     /locus_tag="ACHJWA_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309704.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
BASE COUNT         1888 a         1626 c         1778 g         2065 t
ORIGIN      
        1 tcgatagcga tattgcacgt ttgttactca agctcagaaa gtctcgaaac ttgactgtta
       61 cagagcttgc ccaacgatct ggtgtcagtc aggcgatgat cagcaaagtt gaacgaggca
      121 ccactgatgc gggtgccggg tttgagcaga cgctgaccgg aatgacagtg gggatcgaca
      181 gccgtaatga tattcctgag gggattgcga cgctgggcgc ttttatgggt tattcccatt
      241 cacatatcgg ttttgatcgt ggaggacatg gcagtgtgga cagttattct ctgggcggct
      301 atgccagttg ggaacatgaa agtggtttct atctggacgg tgtcgtgaag ctgaaccgtt
      361 ttgaaagtaa cgtagccggt aaaatgagca gcggtggagc cgccaatggc agttaccata
      421 gcaacgggct gggcggtcac attgaaaccg ggatgcgatt taccgatggt aactggaacc
      481 tgacgccgta tgcctcgtta acggggttca ccgctgataa ccccgaatat catttatcca
      541 atggcatgga atcgaaatca gtcgataccc gcagtatata tcgtgaactg ggtgcaacgc
      601 tgagttacaa catgcgtctg gggaacggta tggaagttga gccgtggctg aaggcggctg
      661 tgcgcaaaga atttgtcgat gataaccggg tgaaagtgaa tagtgacggt aatttcgtca
      721 atgatttgtc gggcagacgt ggaatatacc aggcaggtat taaagcctca ttcagcagta
      781 cgttaagcgg gcatcttggg gtggggtata gcaacggtgc tggtatggaa tccccgtgga
      841 acgcggtggc tggtgtgaac tggtcgttct gaccatcaac gattaaactg cgcttcggcg
      901 cagttttcgt ttacaggatg ttaaaagaga aaattctggg gtaaaaaaag cccgccagtt
      961 acggcgggaa acctcatcct atgggagaac aatgaataat gaaattgcgg ggttatcatc
     1021 tcccagtata tcaacactaa caataaggtt atttactcaa ccaggcataa atattttgtt
     1081 ttgtgcgtgg gaacagcctt aaggtgtaaa gggggaggtg gaaatagcaa tgaggagtat
     1141 cagcaagaat actcgccgct ttaccacaac gtggatgaga gggatgaaaa actcaaggca
     1201 gagataactc tgccttgaag ataaatgcgc ttttacagcg ggattatttc agctcttctg
     1261 cttccggtaa ggtcacgttc agctcaagaa tagaaatatc gccatctttt tgctcaagct
     1321 gtacggttac catctcagga tcaatttgta cgtatttaca aatgacctca agaatatctt
     1381 tacgcaactg cggcagataa tgcggttctg catcgctgcg acggcgttca gcaacaataa
     1441 tctgcagccg ttcttttgca atgttggctg tatttttctt ccgcgagaga aagaaatcga
     1501 gtaatgccat aacttatcct ccgaacaagc gtttgaggaa gcctttcttc tcttcttcaa
     1561 tgaagcggaa aggacgttct tctcccaaca gacgttctac ggtatctgcg taggctttac
     1621 ccgcatcggc gttaatgtcg agaatgaccg gttcaccctg gttagaggcg cgcaatactg
     1681 attgatcctc tgggatcacg ccgacgagtt tgatgcgcag gatctccagc acatcttcca
     1741 tgctcagcat gtcacctctg cttacgcggc ctgggttata gcgcgttaac agcaggtgct
     1801 ctttaatagg ctcttcgcca ttttctgcgc ggcgtgattt tgacgccaga atgcctaaaa
     1861 tacggtcaga gtcgcgtact gaggagactt ccgggttggt ggtaataatg gcttcgtctg
     1921 caaaatagag tgccattaac gcaccggttt caatccctgc cggggagtca caaacgataa
     1981 attcaaaatc catcgctttc agatcatcaa gaactttagc gaccccttca cgggtgaggg
     2041 catctttatc gcgtgtttgc gatgccggca gaatatagag attttcagta cgcttatctt
     2101 taattaacgc ctgatttagc gttgcatcgc cctgaatgac gttgacgaaa tcgtaaacga
     2161 cccggcgttc acaacccata atcaggtcga gattacgcag gccgatatca aaatctatca
     2221 cgacagtttt ctttcccttc tgggccaaac cagtggcgat ggccgcgctg gaggttgtct
     2281 taccaacacc ccctttgccc gaagtaacaa caataatgcg tgccatagaa attccttgtt
     2341 aaaaagggat caatttaacg gttgaacggt caaagcgttt tcgactaact gcagtcgcgc
     2401 cgctttgcca taaaattctg ctgggatttg atcactcagc cagtattcac ctgcgatgga
     2461 caccaattcc gccatcaggt tcgtacaaaa tatttgcgtt tcccggtcac cacttgcccc
     2521 tgccagcgca cgaccgcgca tcatgccata gacatgaatg ttcccatcgg caatcaattc
     2581 ggccccagcg ctaacgtggc ttgtaacaat cagatcacat tgtggagcat aaatacgctg
     2641 accggaacgc accggggtat ctattaaacg cgtttttgtg accggcgttg tattttgcgc
     2701 tggagcctgc ggtgcgggag ctggacgtgg cgctttttcc tttccttccg tcaggatagg
     2761 cagccccatt ttttcaattt cggctttaag ttgcgcatct ttgcagccac ttacgccaat
     2821 aacccgcaaa ccggttgccg aaaccgcctt atgcatcgct gaccagttta ccgggtcttc
     2881 cagtgcactg acgttgagta caacgggggc atgttttaaa aatgcggggg cctgagcgat
     2941 tttgtcttcc agcgcctgat ggataacctt aggttctgcc tcatgcagat gaaccacaga
     3001 taaagtgaag ctactgcctt taagctcgat tggcgtgttt gacatcctgg ccttactcaa
     3061 ttagctatta atcatcgcca gcgcgcgatg atgttccgaa gactataagg catgttatag
     3121 tctggattat attgaggcaa gtcaccctcc catttattca gagtaaaagt ctattctgtg
     3181 ataaatggcg ctgattcata gcttaaaaaa tacccttgtc aatcaaccca ttgccgtcgt
     3241 acttttgatt cttcttattt acgcttcctt tttccgcacc ctaatcgtga cagcatctcc
     3301 agacctgttt aaatatccag tattgaggca agtgtatttt ttcaccacgc caggctttct
     3361 taactttcca ttttttgcag catcattaaa gaacagatac ccgttatctt aatatccgcc
     3421 acagaattgc tctatcatag aaaccgcaga agacgtctga tacttctgtc aggtcatcgt
     3481 aactcgtcca ggtcagcggt tttgattacg gattgatgat gaaatgagca taagtgttgt
     3541 acggttggat tatcaggatg tgctatgaat atgatgcgta ttttttatat cggattgtca
     3601 ggtgtgggga tgatgttctc atcaatggcg tctggacatg acgctggcgg acttcaatct
     3661 ccggcgtgcg gcgttgtttg cgacccgtat atttgtgtga actcagatgg catttcccca
     3721 gagttaacaa ggaaatatct cagcgaaaaa gccgctgaaa acttacaatc attacaaggc
     3781 tacgatccca gcgaatttac attcgctaac ggtgtatttt gcgatgttaa agaaaaatta
     3841 tgccgtgatg atcgttattt tggtgtagat ggtaagcgga gtggaaaaat caatcaaacc
     3901 accacaaaga tgttatttat gtgtcgtgag tgatgtcttg tttacattat gttctattcg
     3961 tcattaacct cttgagcgac agagaggagt ggggcgtcaa cgtttatgac ggggagactc
     4021 cccgtcggtt gacatttact taatttgaat atcgacgtta tattttttag ttttgttttt
     4081 acgggcgtcg tttcgggttt gcagcactcg cagctcatat ttgccagaag cagggagtga
     4141 gtattgtccg tgactatcga gttcagggga atatctggat aaatcaacag aatcatcgat
     4201 tcctggcccg aagaggtaag tatcagcgcc ttcattagag atactcacat gcactttctg
     4261 accttttttg gcatagaagg tatatgtatc gtaatcgtat ccctttattt cgccggagta
     4321 ctgagcgctg ctatggcctt ttctgaactc aacattgaca ttcttacccg cagcaaagct
     4381 cgtggaggtt agcagtgcaa gcagcaatac agccttactg atgctcttga ttttcattgg
     4441 tagtcttctg ttcagaatga gtacgatgaa ctgttataat ataacaatcc cttacgggag
     4501 aattccagtg gctttcctgc gttcggtaaa atcccgtcgc ggcatttttg ccggggggat
     4561 tacttataaa ggggcatgga ctcaacaaac cgtagaaatt tacagctggc tggcttgtgg
     4621 cgcgggtttc attgatgtta gcttatgcca aaacctggca tcttaaagag taaaagtatg
     4681 ttttgtgtga tttatcgaag cagcaagcgt gaccagacct atttatatgt cgaaaaaaaa
     4741 gacgattttt cgcgtgttcc tgaggaactg atgaaaggtt ttggtcagcc tcagttagcg
     4801 atgattctgc cgctggatgg gcggaagaaa ctggtcaatg ccgatattga aaaggttaaa
     4861 caggcattaa ccgagcaagg ttactatttg cagttaccgc caccacccga agatttgctg
     4921 aagcaacatc tttccgtcat ggggcagaaa acagacgaca ctaacaaata accgatatcc
     4981 ggcggtggca ttatctttgt cggcgcgggt tttcatatcc acgataaggt gaggggaacg
     5041 ttatgtatca acatcacaac tggcaaggtg cgctgctgga ttatccggtg agtaaagtag
     5101 tttgtgttgg cagtaactat gccaaacata ttaaagagat gggcagcgca gtgcccgaag
     5161 agccagtgct gtttattaaa ccagaaacgg cactgtgcga tctgcggcag ccattggcga
     5221 tcccatccga tttcggttca gttcatcatg aagtcgaact agcggtgttg attggcgcga
     5281 cgctgcgtca ggctacggaa gagcatgtcc gcaaagccat tgccggttac ggcgtggcgc
     5341 tcgatctgac gttgcgtgat gttcagggaa aaatgaagaa agccgggcag ccgtgggaaa
     5401 aggctaaagc gtttgataac tcttgtccgc tttccgggtt tattcccgcg gcggaattca
     5461 ccggcgatcc gcaaaataca acgctgggcc tgagcgtaaa cggcgaacaa cgccagcaag
     5521 gtacgactgc ggacatgatc cataaaatcg ttccgctgat cgcttatatg agcaagtttt
     5581 ttaccctcaa ggccggtgac gttgtgctga caggcacgcc tgatggcgtc ggcccgttgc
     5641 aaagcggtga tgagctgaca gtcactttcg atgggcattc tttgacaact cgcgttttgt
     5701 aatacttttt gccgcctgaa agcggcggca aaacttgcat cgctgtgcca gactggttat
     5761 aaggtgcgtt ttaacgtaat ggcggaacac ctgatgagcg atgtaccttt ctggcaaagt
     5821 aaaaccctgg acgaaatgag cgatgcggaa tgggagtcgt tgtgtgatgg ttgcggtcag
     5881 tgttgcctgc ataaactgat ggatgaagac accgacgaaa tctacttcac taacgtcgcc
     5941 tgtcgccagc tcaatattaa aacctgtcaa tgtcggaact acgaacgtcg tttcgagttt
     6001 gaacccgact gcattaaatt aacccgtgaa aatctgccaa cattcgaatg gctaccaatg
     6061 acctgcgctt atcgtttgct ggcggaaggt aaagatttac ctgcgtggca tccgctgctt
     6121 actggttcga aagcggcaat gcatggtgaa cgtatctctg tgcgtcatat cgcagtgaaa
     6181 gaatcagaag tgattgactg gcaggatcat atcttaaata aacctgactg ggcgcagtga
     6241 taaatttaat agatgttgat gggggctttc ttaatatacg tctggatgct cgccatttcg
     6301 tagccctgtt tttgaaagcg ctctgtcata atgagagtta cccgatacct tattatcaac
     6361 aggtaactct caatctttaa aaaacgtctg tatttttaaa tgtgataaac tttatacctt
     6421 gagtacatgg agagaataat tgctgatcag acttcaggta cctcaaagag tgtcttctta
     6481 ccgtgtcttt tctgatactc attacaggtg ttaatcattt tattggccgc ttcttttagc
     6541 aaagaaagca ttaaatcatc ataatcaaca tagaatctgg ttgtttcagt ttccgttttt
     6601 atctccccga tggcgctatt tcggtggtta atttcaattt ggcggcatcg atatctttat
     6661 tcgcttgttt aaccgtgtta gacagggtgg taaagaaact ctgcacagat tttaacttgt
     6721 tcttcaattc tggaatcagt ttcccttcaa ctacgcccgc agcaatagaa taggaaatga
     6781 ttaaaccaaa tggaccggcg acgacaccgg ctgcggcacc ggcatacgct tccttcctga
     6841 ttttatctac ctgtgactgg aaatagctgc ttttttctga aaaatcattg gttaactggc
     6901 tatctaacgc cagcagtttc ccggaagcgt tgttgaaact ttgtgagctt accagcaggg
     6961 atttttgcgc ttcattcagc ttcgtgatgc cgtcatccag taccttaatg agaatgtctt
     7021 tctgggcgga tgctttcttc tcattatact catcaaatag caaaatatac gctgcgagca
     7081 attgcgtcgc aacaccacac cattcataca ctgtttgggt tgcttcaaaa tacttatcct
     7141 ggctatccat tagtaaggtt ttaatattgc caactaaaac ggaggctgcc tgtgaatact
     7201 cctgtttaaa gcgacttaac tcttttatgg tttcatcaaa ggtctgccag gggatgacct
     7261 gatcgagata tttattataa agatccaatg ctccatctgc ggtttcgatt gcgtttttaa
     7321 ctacctctac cgttttatct gcaacgattt cagtcat
//
LOCUS       DAZFAB010000167         7207 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZFAB010000167 DAZFAB010000000
VERSION     DAZFAB010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7207
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.167"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(329..1465)
                     /locus_tag="ACHJWA_004449"
     CDS             complement(329..1465)
                     /locus_tag="ACHJWA_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO4310908.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1468..1830)
                     /locus_tag="ACHJWA_004450"
     CDS             complement(1468..1830)
                     /locus_tag="ACHJWA_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFO4310909.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIASLSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2367..4280
                     /gene="mtlA"
                     /locus_tag="ACHJWA_004451"
     CDS             2367..4280
                     /gene="mtlA"
                     /locus_tag="ACHJWA_004451"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFO4310910.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVETLSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            4375..5523
                     /gene="mtlD"
                     /locus_tag="ACHJWA_004452"
     CDS             4375..5523
                     /gene="mtlD"
                     /locus_tag="ACHJWA_004452"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFO4310911.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVDAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISDLDANSEVVSEAVTAYKAMQ"
     gene            5523..6110
                     /gene="mtlR"
                     /locus_tag="ACHJWA_004453"
     CDS             5523..6110
                     /gene="mtlR"
                     /locus_tag="ACHJWA_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFO4310912.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(6122..6331)
                     /gene="yibT"
                     /locus_tag="ACHJWA_004454"
     CDS             complement(6122..6331)
                     /gene="yibT"
                     /locus_tag="ACHJWA_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFO4310913.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            6616..6978
                     /locus_tag="ACHJWA_004455"
     CDS             6616..6978
                     /locus_tag="ACHJWA_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFO4310914.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         1851 a         1798 c         1812 g         1746 t
ORIGIN      
        1 ttgtgatcta tagattaaat attttttaaa tgtgactgtc atccttcaga taataaagcc
       61 atcatcgcaa tagatggaaa agcactcctg ctcttttttg attagagccg tcgcaggagg
      121 aacaactcat atcatcattg cgatctcgac gatgtatagt ctcatcctgc ggcagaacaa
      181 gacggataaa aaatccaatg agagcacact catccagaac ttattaacat aatgaatatt
      241 aaagaaaaat catcgcagct aatgaagtaa tatgcaaaaa taattagtcc ttacaaggag
      301 caaagttcca taatctcccc cctccccctc aatgatccaa ataaagataa tgcatccagc
      361 tggtcattct tagcagcact ttccgcatca ccgaaacatg gctgaaatgg tcggagtaaa
      421 ccgccacctg ggcgtagatg ccgtcgggta aggcatcgat atcatcgtta gggtccagtt
      481 caatggttcc cagcacaccg tccgtgccgg gcacgaccgt taatgattgc aataccccct
      541 gcgcctgata agaaccgcct ggcacgacag gtaaaatact agtcagtttg ccgtgaaaca
      601 cctgcccagg tagcgcgtta aacaccactt ccgcatcatc gccgggtttc agacgtaaca
      661 gcgagttttg ccgaaattgg gcgacaattt gccgtttttg ctcggggatg aagaccatca
      721 ccggacgcag cggcaaggca gctgcgtatg tacctgggcg gatcagtacc tgagtaacgt
      781 agccattgct cggcgcgcga atgacagtct gctcaaggtt atattttgct tcagtaagtt
      841 gcgctcttaa gctcacaatc tgcgattgct cgccgttaac catactgtcg agctggctct
      901 ggatctgcgc ctgctccgcc accgagcctt tcaccagcgc atcctgcgcg aggaaatttt
      961 gccgcgcatc gtcgatgtca cgttccgaga acggattcac cgccgcctgg ctgcctttca
     1021 agtaacgttg ataattttta aacagacggt cgcgctccgc tgaaacctgg gtggtgttgg
     1081 cctgcgcttc agtgagctgc gcacgcagcg tctttatatt atgcgtcgcc gtcatcaggt
     1141 cagcctgaag tctgtcaact cgcgcctggt aacgaaccgg gtcgagctta aaaagcacct
     1201 cgcccttttg aataagctga ttattcttgt cagtgacttc agtaacaatt cccgtcacct
     1261 gtggcgtgat agggatcgct atcactgcct tttgcgcggt aaaagtgtaa gggtggttgt
     1321 agttcatcaa taaaatcaaa ccactcacca gaaacacgcc tcccagcgcc gccgtcgcca
     1381 gcgtccactg atttaccgga atgcggaaga ttttaaagac cgcccacgcc agcgccacgt
     1441 aagttaaaac aatcaataga tccataatta gatctccggg aacgtggaat agtcagtctt
     1501 tttctcggcg gcgagttgat gctccagccg ggaaattcgg tcagaaagtg aggcgatttc
     1561 cgggtcagtc gctttctcct gcgacgcaac atgtgactgc ataccccagc cacgctccgg
     1621 ttgatagagc gtcgcccaga tccacagaaa cggccagata acatgcagag taaacaggct
     1681 cacccagccc gccgtatgaa tagcgtcggc atgggggtga ttgcgctttt tggcaatcag
     1741 ataagggatg tcatgtatcg cgatgattcc ataaaaaatc accagaaaga cgaagatcag
     1801 cactcccaac gcgaaatagt ttagaaacat atctgcctcg gattcacgtt tatcagtgtt
     1861 gtttttgggc tggcagccag aagggagtca ggctgatatt ttgacaataa tccgggttcg
     1921 cgattctcgc cataacacca aagaataatt tttagaggtg atgagttgct tagttacata
     1981 acgattgtat gacgaaggca taacatgctg tagatcacat caggtgaacg ccgtaagaaa
     2041 atatcttgtg attcagatca caaagattca acaaaccatc aaaacaaaaa tgtgacacta
     2101 ctcacattta aatgccattt ttagcgaaaa tcgccgcctt gttgcttttt tacacaagcg
     2161 ttttgtgatg aacgtcacgt caattacctc tctaccccct atatttatgt gattgatatc
     2221 acacaaaagg ccgtcgactg gacagttaac cgattcagtg ccagatttcg cagtatctac
     2281 aaggtccggc tacctctgcc gccacattaa caaaaaacct cgggcttcca gcctgcgcga
     2341 cagcaaacat aagaaggggt gtttttatgt catccgatat taagatcaaa gtgcaaagct
     2401 ttggtcgttt cctcagcaac atggtgatgc caaatatcgg cgcgtttatc gcgtggggta
     2461 tcatcaccgc gttatttatt ccaacagggt ggttaccgaa cgagacgctg gcgaagctgg
     2521 tcgggccgat gatcacttat ctcctgccgc tgctgatcgg ttataccggt ggtaagctgg
     2581 taggcggcga acgtggcggc gtagtcggtg ccatcaccac catgggcgtt atcgtcggcg
     2641 cagacatgcc gatgttcctc ggttctatga ttgcaggtcc gctgggcggc tggtgcatta
     2701 agcacttcga ccgctgggta gacggtaaga tcaaatccgg ttttgagatg ctggtgaata
     2761 acttctccgc aggcatcatc gggatgatcc tcgctattct ggcattcctc ggcattggcc
     2821 cgattgttga aaccctgtcc aaaatgctgg ctgcgggcgt taacttcatg gttgtccatg
     2881 acatgctgcc gctggcgtct atctttgttg aaccggcgaa aatcctgttc ctcaacaacg
     2941 ccattaacca cggtatcttc tcgccgctgg gtattcagca gtcccatgaa ctgggtaaat
     3001 caatcttctt cctgattgaa gctaacccag gtccaggtat gggcgtgctg ctggcgtaca
     3061 tgttctttgg tcgtggtagc gctaaacagt ctgcgggcgg tgcggcaatc atccacttcc
     3121 tgggtggtat ccacgaaatc tacttcccgt atgtgctgat gaatccgcgt ctgatcctcg
     3181 cagtcatcct cggcggtatg actggcgtgt tcacgctgac tatcctgggc ggtggtctgg
     3241 tttctccggc atctccgggt tctatccttg ctgtactggc gatgacacca aaaggtgctt
     3301 atttcgctaa catcgcgggt gtgtgtgcgg cgatggctgt ctccttcgtt gtctctgcta
     3361 ttttgctgaa aaccagcaaa gtgaaagaag aagatgatat tgaagcagca actcgtcgta
     3421 tgcaggacat gaaagctgag tctaaaggtg catctccgct gtctgcgggc gatgtgacta
     3481 acgacctgag ccacgtacgt aaaatcatcg ttgcctgtga cgccggtatg ggttccagtg
     3541 cgatgggcgc aggcgtgctg cgtaagaaaa ttcaggatgc aggtctgtca cagatttcgg
     3601 tcactaacag cgcgatcaac aacctgccgc cagatgtgga cctcgtcatc actcaccgtg
     3661 acctgaccga acgcgctatg cgccaggttc ctcaggcaca gcatatttcg ctaaccaact
     3721 tcctcgacag cggcctgtac accagcctga ctgaacgtct ggttgctgcc cagcgacata
     3781 ctgaaaacga agtgaaagtg aaagacagtc tgaaagacag ctttgacgat tccagtgcta
     3841 acctgttcaa actgggcgcg gagaacatct tcctcggtcg caaagcagca accaaagaag
     3901 aagcgattcg ttttgctggc gagcagctgg tgaaaggcgg ttacgttgag ccggagtacg
     3961 ttcaggcgat gctggatcgt gaaaaactga ccccgactta tctgggtgag tctatcgcgg
     4021 tgccacacgg tacggttgaa gcgaaagatc gcgtactgaa aacgggcgtg gtgttctgcc
     4081 agtacccgga aggcgtgcgc ttcggtgaag aagaagatga cattgctcgt ctggtgattg
     4141 gtattgctgc ccgtaacaac gagcacattc aggttatcac cagcctgacc aacgcactgg
     4201 atgatgagtc cgtcatcgag cgtctggcac acaccaccag cgtggatgaa gtgctggaac
     4261 tgctggcagg tcgtaagtaa tccaatccta ccctctcctc acggagaggg tttgggtgag
     4321 ggaaaagcct caccccagcc ctctcgggta aaaacattga tgaaggttaa tactatgaaa
     4381 gcattacatt ttggcgcagg taatatcggt cgtggcttta tcggtaaact gctggcagac
     4441 gcgggtatcc aactgacgtt tgccgatgtc aatcaggtgg tacttgatgc cctgaatgcc
     4501 cgtcatagct atcaggttca tgtggtcggt gaaaccgagc aggtagatac cgtttccggc
     4561 gtcgatgctg tcagcagcat tggtgatgat gtcgttgatc tgattgctca ggtcgattta
     4621 gtcactaccg ccgttggccc ggttgtgctg gaacgtattg ctccggctat cgccaaaggg
     4681 ctggtgaaac gtaaagaaca aggtaatgaa tccccgctga acatcatcgc ctgtgaaaac
     4741 atggtacgcg gtaccacgca gctgaaaggt catgtgatga acgccctacc agaagacgcc
     4801 aaagcgtggg tagaagaaca cgttggcttt gtcgattccg ccgttgaccg catcgtaccg
     4861 ccttcggctt cggcaactaa cgatccgctg gaagtgacgg tagaaacttt cagcgaatgg
     4921 attgtcgata aaacgcagtt caaaggcgca ctgccgaaca tcccaggcat ggagttaacc
     4981 gacaacctga tggcatttgt cgaacgtaaa ctcttcaccc tgaacacggg tcatgctata
     5041 accgcgtacc tcggaaaact ggccggtcat cagaccattc gtgacgctat tctcgacgag
     5101 aaaatccgcg cggtggtaaa aggtgcgatg gaagaaagtg gtgcggtact gatcaagcgc
     5161 tacggctttg acgcagacaa acatgcggcg tacatccaga aaatcctcgg tcgttttgag
     5221 aacccgtatc tgaaagatga tgtagagcgc gtaggccgtc agccgctgcg taaactgagt
     5281 gctggcgacc gtctgatcaa gccattgctc ggtacgctgg aatacagcct tccgcacaag
     5341 aatctgattc aggggattgc tggtgcaatg cacttccgca gtgaagacga tccgcaggct
     5401 caggaactgg cagcactgat cgctgacaaa ggtccgcagg cggcgctggc acagatttcc
     5461 gatcttgatg ccaacagcga ggttgtatcc gaggcggtaa ccgcttataa agcaatgcaa
     5521 taatggtgga ccaggcgcag gacaccctgc gcccgaataa cagattgtca gatatgcagg
     5581 caacaatgga acaaacccag gcctttgaaa accgtgtgct tgagcgtctg aatgctggca
     5641 aaaccgtgcg aagctttctg atcaccgccg tcgagctttt gaccgaggcg gtaaatcttc
     5701 tggtgcttca ggtattccgc aaagacgatt acgcggtgaa gtatgctgta gaaccgttac
     5761 tcgacggcga cggtccgctg ggcgatcttt ctgtgcgttt aaaactcatt tacgggttgg
     5821 gcgtcattaa ccgccaggaa tacgaagatg cggagctgct gatggcattg cgtgaagaac
     5881 taaatcacga cggcaacgag tacgccttta ccgacgacga gatccttgga ccctttgggg
     5941 aactgcactg cgtggcggcg ttaccaccgc cgccacagtt tgaaccagca gactccagtt
     6001 tgtatgcaat gcaaattcag cgctatcaac aggctgtgcg atcaacaatg gtcctttcac
     6061 tgactgagct gatttccaga atcagcttaa aaaaagcctt tcaaaagtaa gcaacgtctg
     6121 cttactgccc ctctacctgc ttcggccgat aaagccgacg ataatactcc agacgttgta
     6181 gatataacgg cacgctttca tcgggtattc cggacggaat cgcgttacgg ggaggaagtt
     6241 ttttcagata ctcccggaac gcctggcttg atgccatgaa atctacggct ttatcgataa
     6301 gaagcggaac gttttcacct aatttgccca tgatgatatc tccgtattac cccgcgccgg
     6361 gaatgcgcgg ccgccaattt tagtttaggt tcccggtaaa gatgacatta aggaaaacgt
     6421 gctgaatcct caataatcat ccgctgctgt ttattatctt ttttctatag atttcttaat
     6481 taatcaataa attgcgtagt ttttaagcat gttaatgatt acgttacaga actattgcaa
     6541 tgtcatcagt tgtgaacaaa gcacctggtc gcgcatacta ggggctataa atttatcttt
     6601 atcagaagcc atcacatgaa agaagtcgaa aaaaacgaaa tcaaacgtct cagcgatcgt
     6661 ctggacgcca tccgccacca gcaggccgat ctgtcgctgg ttgaagccgc agacaaatat
     6721 gccgagctgg aaaaagagaa agccacgctg gaagcagaaa ttgctcgcct gcgtgaagtt
     6781 catagccaaa aactgagtaa agaagcacag aagctgatga agatgccgtt tcagcgcgca
     6841 attaccaaaa aagagcaggc tgatatgggc aagctgaaga aaagtgttcg cggactggtg
     6901 gttgtgcacc caatgaccgc gctgggccgt gaaatgggcc tgcaggagat gactggattc
     6961 tctaagactg cgttttaagc gcctcagacg actggttcga tcatatcaac ccgcatctgg
     7021 cttgccttag ctggtcaatg cggggactct cctgtctact cgcctaatta ccaaacaatt
     7081 cactcccttt acgtcgattt accgctggtt gatattctga aaatgcattt ttaccgaaat
     7141 agccacctat aaacaataaa aatatataga agatatcatt tattaattaa caagcagtta
     7201 cgtctct
//
LOCUS       DAZFAB010000168         7146 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZFAB010000168 DAZFAB010000000
VERSION     DAZFAB010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7146
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.168"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..645
                     /locus_tag="ACHJWA_004456"
     CDS             <1..645
                     /locus_tag="ACHJWA_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO4310915.1"
                     /translation="SMIFPVHAGMVIFGTRIIYPAEQKEVMVQLMNQGNRSSLMQAWI
                     DDGDTSLPPEKIQVPFMLTPPVVKVAANSGQQIKIKIMPNKLPSNKESIFYLNVLDIP
                     PNSPEDEGKNALKFAMQNRIKLFYRPAGIAPVNKTTFKKLRVSRSGNNVVIKNDSPNW
                     VTISDVKANNVKVNYETIMIAPQESQNVSVKNNNANGWQLTIIDDHGNYISDKI"
     gene            661..3141
                     /locus_tag="ACHJWA_004457"
     CDS             661..3141
                     /locus_tag="ACHJWA_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFO4310916.1"
                     /translation="MLRMTPLASAIVALLLGIEAHAAEETFDTHFMMGGMKGEQVTNL
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPHETCLTREIVKRLGINSDNFAREN
                     QCLTFEQLVQGGSYSWDIGIFRLDLAVPQAWVEELENGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTDNNPGEWKSNALYLEHGF
                     SQILGTLRIGDMYTSADIFDSVRFTGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFSISDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFLSQTSTRFGLAAWRYSSRDYRT
                     FNDYVWANNKDNYRRDKNDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYTLAASQAYDENHAEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGNRDQFNYGINLSHQHQGNETTAGANL
                     TWTAPAATVNGSYSQSSTYRQVGASVSGGLVAWSGGVNLANRLSETFAVMHAPGIKDA
                     YVNGQKYRTTNCNGVVVYDGLTPYRENHLMMDVSQSDSETELRGNRKMTAPYRGAVVL
                     VDFDTDQRKPWFIKALRSDGQPLTFGYEVNDMHGHNIGVVGQGSQIFIRTNEIPPAVN
                     VAIDKQQGLSCTITFGKEIDESKNYICR"
     gene            3152..4186
                     /locus_tag="ACHJWA_004458"
     CDS             3152..4186
                     /locus_tag="ACHJWA_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4310917.1"
                     /translation="MMFRNRILLIFILWANFTWAGCRTTASLNITDGINVGEILANET
                     SFSKSVVFTGISCDTSTDKIVYKNIQSDWVEVGPFGNGEKLKVKIESLGKTSDTIGKS
                     SNAQAVLPYVVKIARGTPDFTGERKSTWFISDTVIANIGGESSSSIDFWLGICKALKF
                     NWCVNYLTSKLAGDTFTLGLNISYYPKNTTCKPENTVIKVDDIALFQLRNQGKIAANS
                     KEGTITLKCDNLFGDKKQASRNMVVYLSSSDLVKGSNTILRGKTDNGVGFVLDLTEPP
                     KGTEAAIKISANGDQGAATSLWKTDKPGVSLNSNIINIPVMASYYVYDEKKVKSGALE
                     ATALINVKYD"
     gene            complement(4268..4606)
                     /gene="rcnB"
                     /locus_tag="ACHJWA_004459"
     CDS             complement(4268..4606)
                     /gene="rcnB"
                     /locus_tag="ACHJWA_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFO4310918.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHYKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(4824..5687)
                     /locus_tag="ACHJWA_004460"
     CDS             complement(4824..5687)
                     /locus_tag="ACHJWA_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFO4310919.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIISTA
                     FWMFWRTWRGERNWLENMHEHHHHDHEHHQDHDHDHDHDHDHDHDHEHHHHHEHGDNE
                     EYQDAHARAHANDIKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTL
                     GATLVVSFSIGLALTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVY
                     MGVHGFMGIMR"
     gene            5808..6080
                     /gene="rcnR"
                     /locus_tag="ACHJWA_004461"
     CDS             5808..6080
                     /gene="rcnR"
                     /locus_tag="ACHJWA_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFO4310920.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            complement(6190..6504)
                     /locus_tag="ACHJWA_004462"
     CDS             complement(6190..6504)
                     /locus_tag="ACHJWA_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein"
                     /protein_id="HFO4310921.1"
                     /translation="MKKLLVTVIFVAMMPNAFALSHGDSATLKEGTFNCKKLTDFYEM
                     ISYIQDNDQQAMLSLITSNKCRVLDESMTVEIQSVDDKGFVSFITPGGHGGWAVKQFF
                     EN"
     gene            complement(6514..6861)
                     /locus_tag="ACHJWA_004463"
     CDS             complement(6514..6861)
                     /locus_tag="ACHJWA_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310922.1"
                     /translation="MKSKIFAALLLLPLSTYASSYKCDALQQGQIGSNGKTVTAQSSI
                     DIDGKQMHFSLNGVSWDLSYIGEKQIAKMYSTESGNVSVSFIDANPIIFVLHLVNDDG
                     RKGTYNISECSEL"
BASE COUNT         2060 a         1397 c         1696 g         1993 t
ORIGIN      
        1 tctatgatat tccccgtgca tgccggaatg gttattttcg ggacccgtat catttacccg
       61 gcagagcaga aagaggtcat ggtacaactg atgaatcagg gaaaccgctc ttcgctgatg
      121 caggcgtgga ttgatgatgg cgacacgtct ctgccgccag aaaagattca ggttcctttc
      181 atgttaacgc caccagtagt gaaagtagcg gccaattctg ggcaacaaat aaaaattaaa
      241 atcatgccta ataaattacc cagtaataag gaaagtattt tttatcttaa tgttctggat
      301 attccaccga atagcccaga agatgaagga aagaatgcgt taaaatttgc catgcaaaac
      361 agaatcaagt tgttttaccg acctgctggt attgctccgg taaataaaac gacttttaaa
      421 aaattacgag tgagtcgtag cggtaataac gtagtcataa aaaatgactc gccaaattgg
      481 gtaacgattt ctgatgttaa ggctaataac gtcaaagtga attatgaaac aatcatgatt
      541 gcaccacaag aaagtcagaa tgttagtgta aaaaataata atgcgaatgg ctggcaactg
      601 accattatcg atgatcacgg caactatatt agtgacaaaa tttaattgca ggagctgcct
      661 atgttgagaa tgaccccgct tgcatcagca atagtcgcgt tattgctcgg cattgaagca
      721 catgcagcag aagaaacctt tgacacccat tttatgatgg gaggaatgaa aggtgagcaa
      781 gtaacaaatt tgcgtcttga tgataatcag cctttacctg ggcagtacga cattgatatt
      841 tatgtcaata aacaatggcg tggaaaatat gagattatcg tcaaagataa cccacatgaa
      901 acttgcctga cgcgtgaaat tgtaaagcgg ttaggtataa atagcgataa ttttgcacgc
      961 gaaaatcagt gtttaacatt tgagcaactt gttcagggcg gaagctactc ctgggatatt
     1021 gggatttttc gtttggatct cgctgttccg caagcctggg ttgaagagct ggaaaatggt
     1081 tacgttccgc cagaaaactg ggagcgggga atcaatgcgt tttatacgtc ttattacgta
     1141 agtcagtatt acagtgacta taaagcctcg ggcaatagta agagcactta tgtgcgtttt
     1201 aacagtgggc taaatttgct gggatggcag ctgcattcag atgccagttt tagcaaaacg
     1261 gataataatc ctggtgagtg gaaaagcaac gcgctgtatc tggagcatgg attttctcaa
     1321 attctcggta cgctgcgtat tggtgatatg tacacctcag ccgatatatt tgattccgtt
     1381 cgcttcaccg gggttcggtt atttcgtgat atgcagatgt tgccaaactc gaagcaaaat
     1441 tttaccccac gggtacaggg gattgcccag agtaacgcgt tggtaactat tgaacagaac
     1501 ggttttgtcg tttatcagaa agaggttcca ccaggtccgt tttcgattag tgatttgcag
     1561 ttagcgggcg gaggggcgga tcttgatgtt agtgttaagg aagccgatgg ttctgtgact
     1621 acgtatctgg tgccttatgc ggcagtacct aatatgctgc aacctggtgt atcaaaatat
     1681 gattttgcgg caggccgtag tcatattgaa ggtgcgagca agcaaagtga ttttgtccag
     1741 gcgggttatc agtatggttt taataattta ttgacgctgt atggcggcac gatggttgct
     1801 aataattact atgcctttac cctcggaaca ggttggaaca cgcgtattgg cgcaatttca
     1861 gtcgatgcaa ctaaatcgca cagtaagcaa gacaatggtg atgtgtttga cggacaaagt
     1921 tatcaaattg cctataacaa atttttgagc caaacatcaa cacgttttgg tctggcggcc
     1981 tggcgttatt cgtcgcgtga ttaccgcaca tttaacgatt atgtgtgggc aaataataaa
     2041 gataattatc gtcgtgataa aaacgatgtc tatgatattg ccgattatta ccagaatgat
     2101 tttggtcgca aaaatagctt ttccgccaat atgagtcagt cattgccaga aggctgggga
     2161 tctgtgtcat taagtacttt atggcgtgat tactgggggc gaagcggcag tagtaaagat
     2221 tatcaactga gttattccaa taactggcgg cggataagct ataccctcgc ggcaagccag
     2281 gcgtatgacg agaaccacgc cgaagagaaa cgctttaata tttttatatc gattcccttt
     2341 gactggggcg atgacgttac gacgccgcga cggcaaatat atatgtctaa ctcaacgacc
     2401 tttgatgatc agggttttgc ttcaaacaat accggattat cgggaaccgt tgggaaccgc
     2461 gatcagttta actatggtat caacttgagc catcaacatc agggaaatga aacgacagct
     2521 ggggccaatt tgacctggac cgcaccggcc gcaacagtga atggcagcta tagtcagtcg
     2581 agtacttatc gacaggtcgg agccagtgtt tcagggggac tggttgcctg gtctggtggc
     2641 gttaatctgg cgaaccgtct ttccgaaacg tttgctgtga tgcatgcgcc gggaatcaaa
     2701 gatgcttatg tcaatgggca aaaatatcgt acaacaaact gtaatggtgt ggtggtgtac
     2761 gacggactga caccttatcg ggaaaatcac ctgatgatgg atgtgtcgca aagcgatagc
     2821 gaaacagaat tacgcgggaa ccgtaaaatg accgcccctt atcgcggcgc ggtcgttctg
     2881 gttgattttg ataccgatca gcgtaagccc tggtttataa aagcattaag atccgatgga
     2941 cagccattaa cgtttggtta tgaagtcaat gatatgcatg gtcataacat tggtgttgtc
     3001 ggccagggca gtcagatatt tattcgcacc aatgaaatac cgccagcggt taatgtggca
     3061 attgataagc aacaagggct ttcatgcaca atcaccttcg gtaaagagat tgatgaaagt
     3121 aaaaattata tctgtcggtg agaggtgaaa tatgatgttc agaaatagaa tattactaat
     3181 atttatattg tgggctaatt ttacctgggc tgggtgtcgt actactgcat cattaaatat
     3241 tacagatggt attaatgttg gggagatttt agcgaatgaa acttccttta gtaaaagtgt
     3301 cgtgtttact gggatatctt gtgatacgag cacggataaa atagtttata aaaatatcca
     3361 aagtgattgg gttgaagttg ggccttttgg taatggcgaa aaattaaagg ttaaaataga
     3421 gtctttaggt aaaaccagcg acacaattgg gaaatccagc aatgcgcagg cagtattacc
     3481 ttatgtggtt aaaatagcca gaggcacacc tgattttact ggagaaagaa aatctacctg
     3541 gtttatttca gataccgtga ttgcaaatat tggcggtgag tcatcgtcat ccatcgattt
     3601 ttggttgggt atttgtaagg cattgaagtt taactggtgt gtgaattatc tcaccagcaa
     3661 actggcgggg gatacattta cgcttgggtt aaatatttcc tattatccta aaaatacgac
     3721 ctgtaagcct gaaaacaccg ttataaaagt agatgatatc gccttgttcc agctcagaaa
     3781 tcagggaaag attgcggcga acagtaagga aggaacaatt acgttgaaat gtgataatct
     3841 tttcggcgac aaaaaacaag catcgcggaa tatggttgta tatctttcta gcagtgactt
     3901 agttaaagga agtaatacta ttttgcgtgg taaaacagat aatggtgtag ggtttgtgtt
     3961 ggatctaaca gaaccaccaa aagggactga ggctgccatt aaaatttcgg ccaacggcga
     4021 tcagggcgcg gcgacatcat tatggaaaac agataaacca ggagtttcat taaatagcaa
     4081 cattattaat ataccagtca tggccagtta ctatgtatat gatgaaaaaa aagttaaatc
     4141 tggcgcactg gaagcaaccg cattaatcaa cgtgaaatac gattaataac aagcaagatg
     4201 agcaagtggc taataataag aaataataag gtgtctgcat ttccccctct ctccgagggg
     4261 gcttttttta gcgatgataa aaaatctcac cgtcgtaggc tttaatgatt ttaccgtcgg
     4321 tgtcgctgat caacacgtac gcgccaccca tataggtcca gtgcgtcccg gcatccggcg
     4381 cgggcaggtt acgcaactgc cactgcttaa tgttgtactc atcggtacgg tacatctctg
     4441 gtacggtgtc ccctggtttg taatgcttaa agtcagcaat gaaactgctt aactcatact
     4501 tagaaattcc cgaggtattg gtcgaacccg cggtggctgg tgcggcccag gcggcactgg
     4561 taaccagcaa aagcgcaccc agcaacatct tatttttaat ggtcataaac tctccacttt
     4621 ggggatctgt aatatcagtc gcatatcttt cctgccatac aatataactg acaagcgata
     4681 ttttgttagt gatcccatga tttcgtaaaa agtgtaaatg aaaggatgta gcaaaagcat
     4741 cctcattgag cgagaagtga gcggaataag ctgatttaag aatcatcctg ggctaaaagg
     4801 atagcgaagc ccaggtgatt gttttatctc ataataccca taaagccatg cactcccata
     4861 tacaccccga ctaaaccaat taacagactg gaaaaatagg gggcgcgttt agcgagagtg
     4921 ttaaatccgc tccagcgttt tgcaacttgt tgaacactga ttgccgctcc aacgcctact
     4981 gtaaccagcg ttagtgccag accgatgctg aaactgacga ccagtgttgc gcccagtgtc
     5041 agggctttca actgaatgca aatcaacagc acggtaattg ctgccgggca gggaataagg
     5101 ccacctgtta agccaaataa caaaatttgc cagttggtga cctctctacc atcaaagcgt
     5161 cgtttaatgt cattggcatg ggctcgtgca tgggcatcct gatactcttc gttgtcgcca
     5221 tgttcatgat ggtgatgatg ttcgtggtcg tggtcgtggt cgtggtcgtg gtcgtggtcg
     5281 tggtcgtggt cttgatgatg ttcgtgatcg tggtgatgat gctcatgcat attctccagc
     5341 cagttgcgtt cgccgcgcca ggtacgccag aacatccaga aagcggtgct aataatgatc
     5401 actgcggaaa tcaactggag ccacggttct gctgattgag cagtaaagcg cttgctgatc
     5461 accatcccgc caaaggcaat caaccagacc actgcggtat gcgaaatggt tgctgccagt
     5521 ccgagcatca ccgcctgttt aatggtgcct ttgatggcga tgataaacgc cgccatcatc
     5581 gtttttgagt gccctggttc caggccatga agcgcaccaa gtaagatggc gctggggatg
     5641 aagaaccagg cgtttccttg ctgaagaaga gttgtaaatt cggtcatgat aataattctt
     5701 agtattaatt cggcaatctg attctactcc cccccagtac ctgatactac cccccagtag
     5761 attaatagtg ctatgatttt tcatgttctt gttaaccagg tgttgccatg tctcatacaa
     5821 tccgtgataa acagaaactg aaagcgcgtg ccagtaagat tcagggccag gtcgtggcgc
     5881 tcaagaaaat gctcgacgag ccgcacgaat gcgctgcagt tttacaacag attgctgcta
     5941 tccgtggcgc ggtaaacggt ctgatgcggg aagtgattaa aggtcatcta acggaacata
     6001 tcgttcacca gggggatgag ctaaaacgtg aagaagatct ggatgttgtt ctgaaggtgc
     6061 tggattctta tatcaaatga aagtgcctaa tatggccgta gttgaggcaa taaatagagg
     6121 gacaagtgac gttaagctga attagagtaa atggcgtgag gacacacgcc attcgaggtt
     6181 atagtttgat taattttcga aaaattgctt cacagcccag ccaccatgac cacctggagt
     6241 tatgaatgaa acaaaacctt tgtcatctac gctctggatt tctaccgtca ttgattcatc
     6301 taacacgcga catttattgg atgtaatgag actcagcata gcctgttggt cattgtcctg
     6361 gatgtaactg atcatctcgt aaaagtcagt taacttttta cagttaaatg taccttcttt
     6421 aagtgttgcg ctgtctccgt gacttagtgc gaatgcattt ggcatcattg ccacaaagat
     6481 gacggtaacg agtagttttt tcattttagc ctcttataat tcagaacatt cactgatgtt
     6541 gtaagtgcct tttctaccat catcgtttac gagatgtagc acgaagatga tgggattggc
     6601 atcaatgaaa gatacagaga cgttcccgct ttcggtgcta tacatctttg caatctgttt
     6661 ttcaccaata tatgagagat cccaactcac tccgttcaag ctaaaatgca tctgtttgcc
     6721 gtcaatatcg atggaggatt gtgccgtaac ggttttccca ttgctgccaa tttgaccctg
     6781 ctgaagagca tcacatttat aggagcttgc ataagtagat agaggtagta gcagaagtgc
     6841 agcaaaaatt ttactcttca tattttccct ttgcttggat ttgggttacc tattttgggg
     6901 tagtcattat aaaactatta attagtaaaa cgaacaagtt ttcatttgtt tagcaatggt
     6961 attgtttagg tttgaaatta ttacgtattt tcatagcaat aggttggata aacagcagtt
     7021 cagggaacaa gttgcactat ctacatactt ttccgctttc cattgcatgt taggtaatag
     7081 atagcctttg cccgtaagac taaaaatatt agaaatgact cattcgaata ccttctattc
     7141 acctta
//
LOCUS       DAZFAB010000169         7055 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZFAB010000169 DAZFAB010000000
VERSION     DAZFAB010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7055
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.169"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..409
                     /locus_tag="ACHJWA_004464"
     CDS             <1..409
                     /locus_tag="ACHJWA_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFO4310923.1"
                     /translation="TLVDRKSRYTIILRLRGKDSVSVNQALTDKFLSLPSELRKSLTW
                     DRGMELARHLEFTVSTGVKVYFCDPQSPWQRGTNENTNGLIRQYFPKKTCLAQYTQHE
                     LDLVAAQLNNRPRKTLKFKTPKEIIERGVALTD"
     gene            complement(417..683)
                     /locus_tag="ACHJWA_004465"
                     /pseudo
     CDS             complement(417..683)
                     /locus_tag="ACHJWA_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494339.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
     gene            841..2025
                     /locus_tag="ACHJWA_004466"
     CDS             841..2025
                     /locus_tag="ACHJWA_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFO4310924.1"
                     /translation="MSETRFHGARVTESTDLVTAINDVDSSVIGIVATADDADAELFP
                     LNKPTLLTRVNDVLGKCGTTGTLYRALKAIADQVSTKVIVVRVAEHKEEGGKTQDQLV
                     IGGSESDGSYTGMYALLVAEQDESIGYRPRILAAPELDTEAVTKSLCVIAGKLRAFVY
                     ATCHGCNTMAEAITYRQKFNEREVMLLWPDFIAYNLKSGKNETFPAPAYACGLRAYID
                     HEQGWHKSLSNVPVKNVLGMSRHVFWSLQAEDSDANSLNNKEITTIIRRNGFRFWGNR
                     TPETNAYIFEVYTRTAQVLADSIAEAQFETIDSPLTPANVKDVISAIRAKLDSLVTAG
                     KLIGAECWYDIEDNSTTNLRQGRVRIRYKYTPVPPLEDMELYQSFTDEFFGPAFAVLG
                     GA"
     gene            2025..2537
                     /locus_tag="ACHJWA_004467"
     CDS             2025..2537
                     /locus_tag="ACHJWA_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFO4310925.1"
                     /translation="MAVPKHLRFFTLFVDGENEVGKVTSVTLPKLTRKTDSYRGGGMM
                     GAVSIDLGLDDSALDASFVMGGAVRELFLKYGGTIDGTLLRFAGEYYTDDESDLYEVE
                     MRGRVTEIDMGEAKQGEATSHTYAIKNTYYKLSVNDRPLWEIDLLNFIYRKDGKDIVP
                     DRIRSALGLG"
     gene            2592..2957
                     /locus_tag="ACHJWA_004468"
     CDS             2592..2957
                     /locus_tag="ACHJWA_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4310926.1"
                     /translation="MKETKNIDTENTVVTDTVKETSERGVKLTQPIERGGEKITYVEI
                     TGAIEQAGSLRDLSLSDVLNLKAESMFTLLSRVTSPRLDEVTIKKMASRDFIQLCVVA
                     VNFLSGADSGGKNEQATEA"
     gene            2966..3121
                     /locus_tag="ACHJWA_004469"
     CDS             2966..3121
                     /locus_tag="ACHJWA_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFO4310927.1"
                     /translation="MCFEHIEDFVADIAVIFNWSPAEIFMMTPGEVVSWRERAALRSG
                     NADNEDS"
     gene            3108..5911
                     /locus_tag="ACHJWA_004470"
                     /pseudo
     CDS             3108..5911
                     /locus_tag="ACHJWA_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
     gene            5924..6412
                     /locus_tag="ACHJWA_004471"
     CDS             5924..6412
                     /locus_tag="ACHJWA_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310666.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4310928.1"
                     /translation="MMMIYGMFVFELRTLPHQQLQQNKSWRHVKNERVNRSASWQYIG
                     AGDDRIVLSGVLYPEITGGEVSLSLLTTQAYTGRPWPLIDGVGQIYGMYVLTGTNTTR
                     SEFDRYGKAKKIEFSLTLERCDEDLRERLQSSSFSDMLSGFKDKVTSSLNSAASSVKG
                     LL"
     gene            6569..>7055
                     /locus_tag="ACHJWA_004472"
     CDS             6569..>7055
                     /locus_tag="ACHJWA_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HFO4310929.1"
                     /translation="MLSYLMAIHFVLFGNSTNLKNFWKHEVIRRKRMDIWRLLREKKQ
                     RNRNFLFWWRLANEMYINGNKLHKKAAKKLNSKIINKFGCEIGLGANIGKGLTIPHHA
                     GIVVHFAVDAGENLVLRQNTTIGQIDGDMPSSRVKIGSNVDIGANCCIIGLSRKIGDN
                     VK"
BASE COUNT         1745 a         1647 c         2011 g         1652 t
ORIGIN      
        1 cacacttgta gaccgaaaat cacgttatac gatcatcctc agactcaggg gcaaagattc
       61 tgtctcagta aatcaggctc ttaccgacaa attcctgagt ttaccgtcag aactcagaaa
      121 atcactgaca tgggacagag gaatggaact ggccagacat ctagaattta ctgtcagcac
      181 cggcgttaaa gtttacttct gcgatcctca gagtccttgg cagcggggaa caaatgagaa
      241 cacaaatggg ctaattcggc agtactttcc taaaaagaca tgtcttgccc aatatactca
      301 acatgaacta gatctggttg ctgctcagct aaacaacaga ccgagaaaga cactgaagtt
      361 caaaacaccg aaagagataa ttgaaagggg tgttgcattg acagattgaa tctacaagtg
      421 cctttttctg tcagcggttc tcctttccag ccaatgagaa ccgtcaggcg cgcgccccgt
      481 ggcggtagct gcaactgacc atctgcatca tccagcgtga tggtgagctg gtccgcctca
      541 aatccccggt tgtcggtcag tgacaggctc atcaggcgct ctgccacgcc tgacagcgtt
      601 ttaccctccg cgagaatatc aaaatccggc actttcacgt ggtctgtgcc ctgactgagc
      661 aattgcatgg tggtgtcggt catctgttcc ctccctgtgc ggcatggtcg catgtgcgtg
      721 cggagggggt tactgctttt tgttgtcgcc gtggcgggag aacggcgcag gggtgagatt
      781 acgcgcgtgg tgggtgatga ttgttgccga atcatttaac ggatacaagg ggctgaagct
      841 atgagtgaaa ctcgttttca tggtgcccgt gttacggaaa gtaccgacct ggtaacagcg
      901 attaatgatg ttgattccag tgttatcggt atcgtggcaa cggcagatga tgcggacgcg
      961 gagctgttcc cgctgaacaa gcccacactg ctgacccgcg tcaatgacgt gctgggaaaa
     1021 tgcgggacta cggggacgct ttatcgtgcg cttaaggcta tcgcagacca ggtgagcaca
     1081 aaagtgatcg tcgttcgcgt ggctgaacac aaagaagaag gcggtaagac gcaggatcaa
     1141 ctggttatcg gtggttctga atctgacggc agctatacgg ggatgtatgc gctgcttgtt
     1201 gcagagcagg atgaaagcat cggataccgt ccgcgtattc tggccgcgcc ggagctggac
     1261 acggaggcgg taacaaaatc cctgtgcgtg attgcgggta aactgcgcgc gtttgtgtat
     1321 gccacatgtc atggttgtaa cacgatggct gaggcgatta cctaccgcca gaaattcaac
     1381 gaacgtgagg tgatgctctt atggcctgac ttcatcgcct acaacctgaa aagtggcaaa
     1441 aacgaaacgt tccccgcgcc tgcttatgcg tgcggccttc gtgcgtacat tgaccatgag
     1501 cagggctggc acaaatcgct gtccaacgtt ccggttaaaa atgtgcttgg gatgtcgagg
     1561 catgtgttct ggtcgttgca ggccgaagac agtgatgcca acagcctcaa caacaaagaa
     1621 atcacgacca ttattcgtcg caacgggttc cgcttctggg gcaaccgcac accggaaacg
     1681 aacgcctaca tctttgaggt gtatacccga accgcacagg tgctggctga ttcaattgcg
     1741 gaagcgcagt ttgaaaccat cgacagtcca ctgacacctg cgaacgtgaa ggatgttatc
     1801 agtgccatca gggcaaaact ggattcactg gttactgccg ggaaactgat tggcgcggag
     1861 tgctggtatg acatcgagga taacagcacc acgaatttac gtcaggggcg tgtgcgtatt
     1921 cgctacaaat atacgcccgt tcctccgctg gaagacatgg agctttacca gtcgtttact
     1981 gatgaattct ttggtcccgc atttgcggtg ctgggaggtg cctgatggct gtaccaaaac
     2041 atcttcgctt ttttacgctg tttgtggatg gtgaaaacga agtgggtaag gtgacgtccg
     2101 tcaccctgcc taaactgacg cgcaaaaccg acagctaccg gggtggtggc atgatgggtg
     2161 cggtaagtat tgacctcggc ctggacgact ccgcgcttga tgcgagcttt gtcatggggg
     2221 gcgcagttcg tgagctgttc ctgaagtatg gcggcacgat tgacggcacg ctactgcgtt
     2281 ttgcggggga atactacacc gatgatgaaa gcgacctgta tgaagtcgaa atgcgcggac
     2341 gtgtgacgga aattgatatg ggggaagcca aacagggcga agccacatca cacacttacg
     2401 ccattaaaaa cacctactac aagctgagtg ttaacgatcg cccgttgtgg gagattgacc
     2461 tgctgaactt catttaccgg aaggacggca aggacattgt gcccgatcgc atccgttccg
     2521 cgcttgggct tggctgataa gtaatatgca ggcggcgcag tgcgtcgcct ctgactgaaa
     2581 ggagtttcct gatgaaagag acgaaaaaca tcgataccga aaacacggta gttactgaca
     2641 ctgtgaaaga aaccagtgag cgtggtgtaa aacttaccca accaattgag cgaggcggcg
     2701 aaaaaatcac gtatgtggag atcaccgggg ctattgagca ggctggatct ctgcgagatt
     2761 tgtcgctgtc tgatgtgctg aatctgaaag cggaatccat gtttacgctg ctgtcacgcg
     2821 tgacatcacc gcgactggat gaagtgacga tcaaaaaaat ggcatcccgt gactttattc
     2881 agttatgtgt ggttgccgta aattttttga gcggtgcgga ctctggcggg aagaacgaac
     2941 aggcgacgga agcctgatca cggttgtgtg ctttgagcac atagaagact ttgtggcaga
     3001 tattgccgtt atttttaact ggtcgcccgc cgaaatcttc atgatgacgc ccggcgaagt
     3061 ggttagctgg cgtgagcggg cggcacttcg cagcgggaat gcagacaatg aagactcttg
     3121 atatccgggt cgctttcagc gccgttgaca ggctgacccg acctgccgaa aacgcacgcc
     3181 gcctgatggg gcagtttggt gactccatcc agcgaacgca gggggcgatc aaaaatctcg
     3241 agcgtcaggc gcgatcattt gagcgcgccc gcgacgctgt cagtaaagcg gatgctggca
     3301 tcgtgaaagc acgacgccag cttaacgccc ttaatcagtt acaacgcacg ggtacagtgc
     3361 tcagcgaaaa acaacaaaag ctgatgcagc agttaagcac ccggcttgaa cgcctgaatg
     3421 aatcgcgcac acgggaaatt cagaaaatga gggaacttgg cggagagctg aaacgccacg
     3481 gcatttccct gacaggcagc gataacacca ttcagcaggc catcagacgc accgaacaat
     3541 acaacaacca gcttgaacgc gaacggcagg cgcttgcgcg tgtaacgcag gcgcgtgagc
     3601 ggtattcgca cgcgcaggaa accgcgggaa aactgaaaac aggtggtgcg ctggcaattg
     3661 gtgcggcagc ggcgggcggc tatgctgccg ggcgtttttt gcagcctgcg atcgggttcg
     3721 gcaaagagat gtcccgcgtt caggcactga cgcgaatcga caaaaacagc ccgcagttta
     3781 aggcgctgcg tgagcaggcg ttaaaacttg ctgaaacaca gtttactgcg agtgatgccg
     3841 ccagtgggca gagctttctg gcaatggctg gttttactcc gcaggccatt caggccgcat
     3901 tgcccggtgt tcttaatatg gcgctggcag gtggcgtcga actcggcgag acggctgata
     3961 taggctccaa tatcctcaca cagttcaacc tgacagccga tcaaatggac cgggtgggcg
     4021 atacgctgac agcagcattt acccggacca atactgattt acgcgcgctg ggcgaaacca
     4081 tgaagtatac cggtccggtt gccgcaaaac ttggtatcag tcttgaagaa gcggcggcca
     4141 tggccgggat gcttgccaat aatggtcttc gcggaagcga tgctggcacg gccatgcgcg
     4201 caagtctgtc ccgccttgca tcaccgccaa aagctgcggc tgatgcactg aaagagctgg
     4261 gggtgtcagt tgctgacgcc agaggcaaaa tgcgcccgat ggaggatgtg ctgcttgatc
     4321 tctataaggc gacacaaaaa tacggacagg tggaccaggt ctccttcttc aaggacatcg
     4381 ccggagaaga ggcgttcgtt ggtttgcaga cgcttgttgc ggcggctggt tcaggagagc
     4441 tgcaaaaact gaccagagaa ttgcaggggg caaggggaga ggccgatcgc gtcgcaaaag
     4501 taatggctga taatcttgat ggggacctga aaaatctcga cagcgcatgg gaaggtcttc
     4561 gtattcgcat cagtgatctg gttgacggtc cgctgcgttc tgtcacgcag tggctcacgc
     4621 gggtgcttga aaaaatcacc tcgctggcgc aggcccatcc ggtactgacg cgccagctac
     4681 tgatagcagg cggtgcgttg ctggcaatga ctgcaacgat tggctcgttg tcgctggtta
     4741 ttggggtgct ttacgggaag ctggccaccc tgcgtcttgg ttttgacatt cttacccggt
     4801 caatgaatgt cgtcagggtg ttacctgcgc tatggggaat ggtgacgggt tccgtttctt
     4861 tactgggagg cgttatcggg gcgttgttca gtccggttgg tcttatcgtg gctgcgcttg
     4921 ccggagctgc cgttcttatc tggaaatact gggatcctat cagggctttc tttgccgggg
     4981 tgttcagcgg gattatggaa aggctgaccc cgttgcgcga cacctttgaa cggtttggcc
     5041 ctgtttttga cgtaatcgga agcggttcag ccaggtgttt aactggttta aatcgctgct
     5101 gtcaccgatg gagtccagca aggaaacgct ggataaatgt accagtgctg gcgaggtatt
     5161 cggtaacgtt cttggtggtg cgttacagct tgttctgacg cctgcaaaaa tgttgctgga
     5221 tacgctggcg tggatacttg aaaaactcgg tgtgcttccg gatgaagcgg aaagggcgcg
     5281 caagaaaatc gaagacgcac aacgtgcggc cattcttcag gacaaggttg ctctgtttca
     5341 gggagacatt gcgaaaatca atccgccgaa gtctgcggga aatagcaatg gcaccggaga
     5401 tgataaaccc aaagacaaca aacctctcac agacagcaat accggcacgc tacgcagact
     5461 cagcaaaatt gctgataaca caggtaagct ggttgatgag acaaaaaaac gcattggccc
     5521 cggcgatatt gtctttaaga acctgccccg cgcacttgct gttcgtgggg agtggcagga
     5581 gcggaagatt gcgcaggtca gtaagcctgc ccccgcaatt aatatcacac ccgtggtccc
     5641 ggctccgctg cctccggcgc tggtccctgt tgttgcggcc agctcccgcc cggtggtgga
     5701 ggccatacga tcgccagtgg catcagttcc tgcaacttcc cgtaaccggg agcctgttgc
     5761 ctccggattt ggcggtgaaa ttcatgttca tctgcataac gttgttacgc agaatccccg
     5821 cgaactggcg aaactggtcg gcgaaatggt cagggcagaa atggaacggc gcgcccgtgc
     5881 caggcgtggc agtttttacg ataaagattg aggagtcatg gccatgatga tgatctacgg
     5941 catgtttgtt tttgagctgc gcacgttgcc gcatcagcag ttacagcaaa acaaaagctg
     6001 gcgacatgtg aaaaatgaac gcgttaaccg ttcagcaagc tggcagtata tcggtgcagg
     6061 tgatgatcgc atcgttcttt ctggtgttct ttatcctgag attactggtg gcgaagtgtc
     6121 gctgtcgttg ttgaccacgc aggcatatac aggacgtcct tggcctttga ttgatggcgt
     6181 cgggcagatt tacggcatgt atgtcctgac cggaacgaat acgacccgct ccgagtttga
     6241 tcgctacggt aaggcgaaaa agatagaatt ttcactgact cttgaacgct gtgatgagga
     6301 tttgcgggag cgcctgcaat cctcatcgtt cagcgatatg ctgtccggct tcaaagataa
     6361 ggtgacatca tcccttaata gcgcggccag ctcagttaag gggctgctct gatttaacgt
     6421 atgtcgccaa tttcctgatg aaggtgactg gcgacttgct gttgtatgtc cttctcagaa
     6481 aattgttttt gaataacaaa taacaggatt ttataatctc ttaaccttat aacatgtgtg
     6541 gtctgaaata ataattaagg agattatcgt gctatcttac ttaatggcaa ttcactttgt
     6601 tttatttgga aactctacta atttaaaaaa cttctggaaa catgaagtaa ttcggcggaa
     6661 acgtatggat atctggaggc ttttaagaga gaaaaagcag cgtaaccgga atttcctttt
     6721 ctggtggcgg ttggctaacg aaatgtatat taatggtaat aaattacata agaaagcagc
     6781 caaaaagtta aacagtaaaa taattaacaa gtttggttgt gaaataggat tgggcgcaaa
     6841 tattggaaaa gggttgacaa ttccccatca tgctggaatt gttgttcatt ttgctgttga
     6901 tgcgggtgaa aatctggtat tacgacagaa tactaccatt ggacagatag atggtgatat
     6961 gcctagttca agagtaaaaa ttggtagtaa cgttgatatc ggagctaatt gttgcatcat
     7021 tggattatca cgtaaaattg gggataatgt aaaaa
//
LOCUS       DAZFAB010000170         6917 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZFAB010000170 DAZFAB010000000
VERSION     DAZFAB010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6917
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.170"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(50..1426)
                     /locus_tag="ACHJWA_004473"
     CDS             complement(50..1426)
                     /locus_tag="ACHJWA_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFO4310930.1"
                     /translation="MRQDIEASVIGGLLIGGLTPTASDVLATLEPEAFSIPLYRKAFE
                     VIRKQARNRNLIDALMVAEACGEEHFTSILMTSKNCPSAANLKGYAGMVADNYHRRLV
                     LEIMDEMREPIQSGTIDASSQAMDELVKRLSAIRKPRDEVKPVRLGEIITDYTDTLDR
                     RLRNGEESDTLKTGIEELDAITGGMNAEDLVIIAARPGMGKTELALKIAEGVASRVIP
                     GSDVRRGVLIFSMEMSALQIAERSIANAGRMSVSVLRNPASMDDEGWARVANGMSQLA
                     DLDVWVVDASRLSVEEIRSIAERHKQENPNLSLIMADYLGLIEKPKADRNDLAIAHIS
                     GSLKAMAKDLKTPVISLSQLSRDVEKRPNKRPTNADLRDSGSIEQDADSIIMLYREAV
                     YDENSSAAPFAEIIVTKNRFGSLGTVYQRFCNGHFVACDQDEARQICTASNAPAARGR
                     RYAQGADV"
     gene            complement(1423..2244)
                     /locus_tag="ACHJWA_004474"
     CDS             complement(1423..2244)
                     /locus_tag="ACHJWA_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO4310931.1"
                     /translation="MGVVKLADYRPQLEVVEHRVADTEDGFMRVANEITDSLLMADLT
                     VRQLKVMLAIMRKTYGFNKPMDRLTNTQIAAMTGIHHTHVCAAKRQLIERKFLIADGV
                     KIGVNKVVSQWISQDSLTLAKTANKTLAKSANGYKPSQLNTKDNIQKTINTNTPLPPN
                     GDGDGQVKPERRKAERIDYESFLNAYNTEVGDRLPHAVAVNEKRKRRLKKIIPQLKTP
                     NVDGFRAYVRAFVHQAKPFYFGDNDTGWTADFDYLLREDSLTGVREGKFADRGIA"
     gene            complement(2231..2392)
                     /locus_tag="ACHJWA_004475"
     CDS             complement(2231..2392)
                     /locus_tag="ACHJWA_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310932.1"
                     /translation="MTKRRKKYQEKEEIRHPDSPEGLVVAAANNRAFAERLVGVYRLA
                     KAGVKHGRR"
     gene            complement(2425..2721)
                     /locus_tag="ACHJWA_004476"
     CDS             complement(2425..2721)
                     /locus_tag="ACHJWA_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HFO4310933.1"
                     /translation="MERTSYSKLSQRDVDRAETDLLINLSTLTQRGLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFKYALDEITKKKSPAATEDFKQIDMQF"
     gene            complement(2863..3078)
                     /locus_tag="ACHJWA_004477"
     CDS             complement(2863..3078)
                     /locus_tag="ACHJWA_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4310934.1"
                     /translation="MSNLRKYRESLNISQTTLAKAVGCTQGAIGHWESGRRFPDLKTC
                     RALVACLNKLGAKVSLDDVFPPEHKAA"
     gene            3153..3848
                     /locus_tag="ACHJWA_004478"
     CDS             3153..3848
                     /locus_tag="ACHJWA_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFO4310935.1"
                     /translation="MDGSSTERNNKDMKMKWYELARSRMKELGITQEKLAEELGMTQG
                     GIGHWLRGSRHPSLSDIGVVFKYLGIDNISFNHDGTFSPVGEYSSAPVKKQYEYPVFS
                     HVQAGMFSPELRTFTKGDAERLVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPDGML
                     ILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSVVG
                     KVIASQWPEETFG"
     gene            4350..4871
                     /locus_tag="ACHJWA_004479"
     CDS             4350..4871
                     /locus_tag="ACHJWA_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310936.1"
                     /translation="MAKSNVSVQAFKDFLEELMSLNIMKEATARNLKNSSARLLTVVQ
                     EEEMGDVTQLDVNELAERYINATEPKPSDSSITAYKSRMESAIKKFVAFQSGEEIPYT
                     PIDKESSEEKDLTGEPTKVEGKANALHTYDLPVVLRPESGVTVTIKGIPNDITNEEAE
                     RISSILKVYVRPQ"
     gene            5440..5622
                     /locus_tag="ACHJWA_004480"
     CDS             5440..5622
                     /locus_tag="ACHJWA_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310937.1"
                     /translation="MEQTGRLFKQRRLSTTWLKSQITQPHKLWDAMPKQPSQEELRDC
                     IAKVYSGGIYVQKNRI"
     gene            5600..5872
                     /locus_tag="ACHJWA_004481"
     CDS             5600..5872
                     /locus_tag="ACHJWA_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein N"
                     /protein_id="HFO4310938.1"
                     /translation="MSRKTEFKGTAASRRRARRANLQSQEAISSDKLHRPTPSRVVLQ
                     CKRKPAMRAEVITLTTLTRKYEGSTCLPNVALYAAGYRKSKQLTAR"
     gene            5931..6182
                     /locus_tag="ACHJWA_004482"
     CDS             5931..6182
                     /locus_tag="ACHJWA_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310939.1"
                     /translation="MEFHESAICDFRANANSVKPQPIAVLFKTMGAWAVLCFAADDTD
                     ARMAIGQEMEMDPTNDEFIIYGAPSNYLLDTCNIYNKAA"
     gene            6365..6733
                     /locus_tag="ACHJWA_004483"
     CDS             6365..6733
                     /locus_tag="ACHJWA_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2528 family protein"
                     /protein_id="HFO4310940.1"
                     /translation="MSNIKKYIIDYDWKASIEIEIDHDVMTEEKLHQINNFWSDSEYR
                     LNKHGSLLNAVLIMLAQHALLIAISSDLNAYGVVCEFDWNDGNGQEGWPPMDGSEGIR
                     ITDIDTSGIFDSDDMTIKAA"
     gene            6806..>6917
                     /locus_tag="ACHJWA_004484"
     CDS             6806..>6917
                     /locus_tag="ACHJWA_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HFO4310941.1"
                     /translation="MQYAIAGWPVAGCPSESLLERITRKLRDGWKRLIDIL"
BASE COUNT         1889 a         1629 c         1560 g         1839 t
ORIGIN      
        1 agaaccatta gtaacgataa ccctgctatt agctcagtga tgtagatggt catacgtcag
       61 ccccttgtgc atatcgtctg ccacgcgcag caggtgcatt tgatgctgtg caaatctgtc
      121 tggcttcatc ctggtcacat gcaacaaagt gtccgttaca gaaccgctgg taaactgtac
      181 caagcgagcc aaaacggttt ttcgtcacga tgatttcagc aaatggcgcg gcgctactgt
      241 tctcatcata taccgcttcc cgatagagca tgatgattga gtctgcgtcc tgttcaatgc
      301 ttcctgaatc acgcaaatct gcgtttgtcg ggcgtttgtt tggtcgcttc tcaacatcgc
      361 gcgaaagctg actcagggag ataaccggtg ttttcaggtc tttcgccatc gccttcaggc
      421 ttccggagat gtgagcaatt gcgaggtcgt tgcggtctgc tttcggcttc tcaatcaggc
      481 caagataatc cgccatgatg agtgacaggt ttggattttc ctgcttatgc cgttctgcga
      541 ttgagcgtat ttcttcgacc gataaccgcg aagcatcgac tacccataca tccaaatctg
      601 caagctgact catgccgtta gcaacacgtg cccagccctc gtcatccatc gatgcaggat
      661 ttcgcagtac gctaaccgac atcctcccgg cgttggcaat gcttcgctct gcaatctgca
      721 atgcgctcat ttccattgag aaaatcaata ccccgcgccg gacgtcagaa ccaggaataa
      781 cgcggcttgc cacaccttcg gcaatcttca gcgccagttc ggttttcccc ataccaggac
      841 gagcggcgat aatcaccagg tcttctgcgt tcatccctcc agtgatagcg tcaagctctt
      901 cgattccggt cttcagggta tctgactctt ctccgttcct cagacgcctg tcaagcgtgt
      961 cagtgtagtc agtgatgatt tcccctaacc gtacaggttt aacctcgtca cggggctttc
     1021 tgatggctga aagacgcttt acaagttcat ccatcgcctg actcgatgcg tcgatggttc
     1081 cgctttggat tggttcacgc atttcatcca tgatttccag caccagacgg cggtgatagt
     1141 tatccgcgac cattccggca tatcccttca ggtttgcggc actcgggcag tttttgctgg
     1201 tcatcaggat tgacgtgaaa tgctcctctc cgcacgcctc ggcaaccatc agcgcatcga
     1261 ttaggtttct gtttctcgcc tgcttgcgaa taacctcgaa ggctttccgg tagagcggaa
     1321 ttgaaaacgc ttccggctcc agcgttgcca gaacgtcact ggcggttgga gttaatccac
     1381 caatcagcag gccaccgata acgctagctt cgatatcctg tctcatgcaa tccccctgtc
     1441 tgcaaacttc ccttcccgaa ctcccgttaa cgaatcttcc ctcagcaggt aatcaaaatc
     1501 tgccgtccag cccgtgtcgt tgtctccgaa gtaaaacggc ttggcctgat gcacaaacgc
     1561 cctgacatac gctctgaaac cgtccacgtt tggcgttttc agttgcggga taattttctt
     1621 caggcggcgt tttcgtttct cgttgaccgc aacagcgtgt ggaagtctgt caccgacttc
     1681 ggtgttgtag gcgttcagga aggattcgta gtcgattcgt tctgccttgc gacgttcagg
     1741 tttaacctgc ccatcgccat ccccgttagg gggtaagggg gtatttgtat ttattgtctt
     1801 ttgtatattg tcttttgtgt ttagctgact tggcttatac ccattagccg acttggctaa
     1861 tgttttatta gctgttttag ctaatgttaa gctgtcctgg ctaatccact gagaaaccac
     1921 cttgttcact ccgattttca cgccatcagc aatgaggaat ttacgctcaa taagctggcg
     1981 cttggcagcg caaacatgag tgtgatgaat acctgtcatg gctgctatct gcgtgtttgt
     2041 gagtcgatcc atcggcttat tgaatccgta tgtcttgcgc atgatagcga gcatcacctt
     2101 caactgccgg acggttaaat cagccatcag cagactgtcg gtaatctcgt tagcaacgcg
     2161 catgaaacca tcttcggtat ctgccacgcg atgctccacg acctccagtt gaggcctgta
     2221 atcagctaac ttaacgacgc ccatgtttca ctcctgcttt ggctagtctg taaacaccaa
     2281 caaggcgctc tgcgaacgcc ctgttatttg ctgcggctac cactaatccc tcaggtgaat
     2341 cagggtgtcg aatctcttct ttttcctggt atttcttacg acgttttgtc ataattactc
     2401 ctgtggattg atccagtaat tccctcagaa ttgcatatca atttgcttaa aatcctcggt
     2461 ggcagccggg gattttttct ttgtgatttc atcaagcgca tacttaaaag ccctgctaat
     2521 cggactgatg tctgatgcca ttccgaaagc acacaagacc gaagcaataa accgccagtc
     2581 cgttctgctt atcttcgatt catgacagcc aatcatcttt gccagaccgc gctgtgtaag
     2641 cgttgacagg ttgatgagta aatctgtttc tgcgcgatca acgtcgcgct gtgatagttt
     2701 gctgtaactt gttcgttcca tttcttaaga tttccaatag tgaatagtta gttgaaaggt
     2761 atgcgtggaa acgcatatgg ccttagttgg tcagatatct tgggactcgc ttttcagcga
     2821 cgtaggacga atgtccgttg ttacaaagag cggctccgct tattaagcgg ctttgtgctc
     2881 cggcgggaac acgtcatcaa gacttacttt tgcgcctaac ttgtttagac acgcaacaag
     2941 agcacggcat gttttaaggt ctgggaagcg acgaccagat tcccaatgtc cgatagctcc
     3001 ctgtgtgcat ccaactgcct tagcaagtgt tgtttgagag atattcagtg actctcgata
     3061 ttttcgtagg ttgctcatat gccctccata gtaaccacga ataaaaaata caatatgtac
     3121 ttctcaaata caagtaaaaa tacacattgt gcatggatgg ttccagtaca gagcgtaata
     3181 ataaggacat gaaaatgaaa tggtatgaac tggctagatc cagaatgaaa gagctcggca
     3241 taactcaaga gaagttagcc gaagagctag gtatgacgca gggtggaatt ggacactggt
     3301 tgcgcggatc tcgtcatcca tctcttagtg atattggtgt ggtgtttaaa taccttggta
     3361 ttgataacat atcattcaac cacgacggga cattttcacc tgttggcgaa tactcatcgg
     3421 ccccagttaa aaaacaatat gagtaccctg ttttttctca tgttcaggct gggatgttct
     3481 ctccagaact cagaaccttt accaaaggtg atgcggagag attggtaagc acaaccaaaa
     3541 aagccagtga ctctgcattc tggcttgagg ttgaaggtaa ctcaatgacc gcaccaacag
     3601 gttccaaacc tagttttcct gacgggatgt taattctggt tgaccctgag caagctgttg
     3661 agcccggcga tttctgcata gccagacttg gtggtgatga atttaccttc aagaaactga
     3721 tcagggatag cggtcaggtg tttctacaac cactaaaccc gcaatatcca atgatcccat
     3781 gcaatgagag ttgttccgtt gtggggaaag ttatcgccag ccaatggcct gaagagacgt
     3841 ttgggtaaag aggatagatg gagtttaatg gccatgagta tcaagcggtc aaaaagaagt
     3901 tcatctattc attgaaagca taaggccatc tgagtatatc cgtaatgatc tggatattgt
     3961 ctataacatc aatgaccgga cgatagatat cggtgagcag cgtcctgtat ggcagggtga
     4021 ggcaggtgaa aaaacgtcct gccatcaaca agaatcaagt gcatccgctc tctggacaga
     4081 tggaaaattt attggatgca aaaagatatg aaataacacc tgtatagcac ggagctttct
     4141 ctgaccgatg cacttgaagt tgttagggct gacccagcct gctgtttctt taggtaagag
     4201 aaaagagatt taggagatga aaggtcgcag aggtgcggcc ttttttattg agagtggatc
     4261 ttgaacggaa attctcaaga cttgagtctt gcatgcaaat caattcctgg ataaactcga
     4321 tctgagtcaa taacttagag agagagcaaa tggcaaagtc aaacgttagc gtgcaggcat
     4381 tcaaggactt ccttgaagag cttatgtcgc tgaacataat gaaggaggcc accgctcgaa
     4441 atttaaaaaa ctcatccgct cgcctcttaa cggtagtcca agaagaggaa atgggtgatg
     4501 ttactcagct tgatgtgaat gagcttgccg agcgatacat caacgcaact gagccgaagc
     4561 ctagcgacag cagcattact gcatataaaa gccgcatgga aagtgcaatc aaaaagtttg
     4621 tagctttcca gtctggtgaa gaaatcccat acactccgat tgacaaagaa tccagtgagg
     4681 aaaaagattt gactggcgaa ccaacaaaag tcgaaggcaa ggctaatgca cttcatacct
     4741 atgatcttcc agtagttctt cgacctgaat caggggttac agtaacgatt aaaggcattc
     4801 ctaacgatat cacaaacgaa gaagccgaac gcatctcttc aattctgaag gtttacgttc
     4861 ggcctcaata atgcaaaagc attcagcaca atgtccaact ccccagtccg acattgatgc
     4921 tgtttaacca gagcctccaa aaggagccct tgttaaggta cacaatattt gcgatgtaac
     4981 cttagcgcgt ctggtacatt ttttcaagcg gttgtagggc tgccgccaat atgagaaaac
     5041 acagatgtct acatacaatt tgaatgacca attcgatcga gaagttcatg tgaatgccta
     5101 tgaacggata aggcatggaa atcttgaaca tgtgtgtgag cattatcgct caaggccaca
     5161 ccgctaatca tcaacccggc ctccatgccg ggttttcttt tcctctcgcc caaaaaaaca
     5221 cataaccaat tgtatttatt ggaaaataaa tagatacaac tcactaaaca acgcaattct
     5281 gatctctcct tacatcgccg aggcaataca cccacgctaa aaaacaatac tattaaatac
     5341 aaagcgttgt aaaaaaacgc cccgttttag aacaaattgt attgaccaag tgaagtacat
     5401 atcgtactat ttaaccatca gcaggacgct ggaagccaaa tggaacagac tggcaggctc
     5461 tttaaacaac gtcgactctc gactacgtgg ctgaaaagcc agatcaccca accacataag
     5521 ctgtgggatg caatgccgaa gcaaccgtct caggaggagc ttcgagattg catcgccaaa
     5581 gtttattcgg gaggaatcta tgtccagaaa aacagaattt aaaggcaccg cagcttctcg
     5641 ccgtagagct cgtcgcgcaa acctgcaaag tcaggaggcg attagctccg acaaactaca
     5701 caggccaacc ccatcacgag tggtcttaca atgcaaacgc aaaccagcaa tgagagcaga
     5761 agtaataaca ctgacaacgt tgaccagaaa atatgaaggc tcaacttgtc ttccaaatgt
     5821 agctctttac gcggcaggct accgtaaatc caaacaactg acagcaagat gacttgtgtt
     5881 ggtcgccaga aaatgaaatt aggcagcaaa ccacttattt gaggtgatat atggaatttc
     5941 atgaaagtgc gatttgtgat tttcgcgcta acgcaaattc agtaaaacca cagccaattg
     6001 cagttctttt taaaacaatg ggtgcgtggg ctgttttatg cttcgccgct gacgacactg
     6061 acgcaagaat ggcaatagga caagagatgg agatggaccc gacaaacgat gaattcataa
     6121 tttatggcgc tccatctaat tacttacttg atacctgcaa catttacaac aaggctgcct
     6181 gatggtggcc tttatttttg gcataaacaa cagaataaac acagcactgt gtattcattc
     6241 caacgagtga atacacggag caatgtcgct cgtaactaaa caggagccga cttgttctga
     6301 ttattggaaa tcttctttgc cctccaatgt gagggcgatt ttttatctgt gaggatatga
     6361 acagatgtca aacatcaaaa aatacatcat tgattacgac tggaaagcat caatagaaat
     6421 tgaaatcgac catgacgtaa tgacagagga aaaacttcac cagattaata atttctggtc
     6481 agactctgaa taccgactca ataaacacgg ctctttatta aatgctgtat taatcatgct
     6541 ggcgcaacat gctctgctta tagcaatttc aagcgactta aatgcatatg gtgttgtgtg
     6601 tgagttcgac tggaatgatg gaaatggtca ggaaggatgg cctccaatgg atggtagcga
     6661 aggaataaga attaccgata tcgatacatc aggaatattt gattcagatg atatgactat
     6721 caaagccgcc tgagcgcggc gttaccgcat accaataacg cttcactcga ggcgtttttc
     6781 gttatgtata aataaggagc acaccatgca atatgccatt gcagggtggc ctgttgctgg
     6841 ctgcccttcc gaatctttac ttgaacgaat cacccgtaaa ttacgtgacg gatggaaacg
     6901 ccttatcgac atactta
//
LOCUS       DAZFAB010000171         6808 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZFAB010000171 DAZFAB010000000
VERSION     DAZFAB010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6808
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.171"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(678..1484)
                     /locus_tag="ACHJWA_004485"
     CDS             complement(678..1484)
                     /locus_tag="ACHJWA_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO4310942.1"
                     /translation="MSGSVIHSQSAVMVPAVYSAGQPASLPVAIDYPAALALRQMSMV
                     HDELPKYLLAPEVSALLHYVPNLHRKMLLATLWNTGARINEALALTRGDFSLAPPYPF
                     VQLATLKQRTEKAARTAGRMPAGQQTHRLVPLSDSWYVSQLQTMVATLKIPMERRNRR
                     TGRTEKARIWEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIP
                     LKVLQSLMGHKSISSKEVYTKVFALDVAARHRVQFAMPESDAVAMLKQLS"
     gene            complement(1485..1790)
                     /gene="ccdB"
                     /locus_tag="ACHJWA_004486"
     CDS             complement(1485..1790)
                     /gene="ccdB"
                     /locus_tag="ACHJWA_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HFO4310943.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1792..2010)
                     /gene="ccdA"
                     /locus_tag="ACHJWA_004487"
     CDS             complement(1792..2010)
                     /gene="ccdA"
                     /locus_tag="ACHJWA_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HFO4310944.1"
                     /translation="MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERW
                     KAENQEGMAEVARFIEMNGSFADENRDW"
     gene            2553..3152
                     /locus_tag="ACHJWA_004488"
     CDS             2553..3152
                     /locus_tag="ACHJWA_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001495930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO4310945.1"
                     /translation="MTSLSPDTVRRIEDAAAALIAAGTPNPTNEQVRQHLGGGSLSHI
                     SPVMRAFRARQREQATPLPPELAQLLTGQLGLLWQAAVKQAEAGALAAREQADDDIAR
                     ADKERDEALANVAALESELAVLREVVAERDRLLQEVRELRAEALPLREQVARLTATGE
                     HLAAQLQDTKAELKEAREDGRQLQTELLALARQDGKVKK"
     gene            3149..3769
                     /locus_tag="ACHJWA_004489"
     CDS             3149..3769
                     /locus_tag="ACHJWA_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310946.1"
                     /translation="MRQERVLRIKAFRRALELGGGERADGCRHFPRWKTELRNFPHSS
                     CDLASNTLAYYLKERERCHPCIIFMEGNAGFHEAENSTVHGHVIVLLDGEYIDLTLDQ
                     FDEYPDYIPYEPVESDGQIGKLLRNIMKHEDPVKTRQIDLDGGEELYAWLRDTADEVL
                     AADPEWQARERSIEEAREAAITVFPFLSDVQKTECEQGPDSQEAAR"
     gene            3766..3966
                     /locus_tag="ACHJWA_004490"
     CDS             3766..3966
                     /locus_tag="ACHJWA_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310947.1"
                     /translation="MKIYHYTDLNGLKGIIESGSLWATHFSFLNDSNELTHGMNCLEN
                     ALQYLRDDFNPKTLKFIEQAPL"
     gene            4374..5351
                     /locus_tag="ACHJWA_004491"
     CDS             4374..5351
                     /locus_tag="ACHJWA_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFO4310948.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PVSLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKLFCIHYRRPR
                     LKAPNDESKENPLPPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            complement(5636..6376)
                     /locus_tag="ACHJWA_004492"
     CDS             complement(5636..6376)
                     /locus_tag="ACHJWA_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4310949.1"
                     /translation="MNNVIPLQNSPERVFLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLDGARPFVRILSEK
                     VRARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAV
                     RRAEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFAL
                     DMAATLAVPFTGDGRDAAEILRTLPPLR"
     gene            complement(6497..6727)
                     /locus_tag="ACHJWA_004493"
     CDS             complement(6497..6727)
                     /locus_tag="ACHJWA_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HFO4310950.1"
                     /translation="MWILNVFQVHKSVRIYIAHFSVFNCHLHPSIDRSLASWHYPNSP
                     KNGKPALSPRHDWLNTGSLLQNVPLRIWFYPQ"
BASE COUNT         1696 a         1793 c         1737 g         1582 t
ORIGIN      
        1 acaatttttt agattgtcac actaaataaa aaagagtcaa taagcaggga taactttgtg
       61 aaaaaacagc ttcttctgag ggcaatttgt cacaacacct tctctagaac cagcatggat
      121 aaaggcctac aaggcgctct aaaaaagaag atctaaaaac tataaaaaaa taattataaa
      181 aatatccccg tggataagtg gataacccca agggaagttt tttcaggcat cgtgtgtaag
      241 cagaatatat aagtgctgtt ccctggtgct tcctcgctca ctcgagggct tcgccctgtc
      301 gctcgactgc ggcgagcact actggctgta aaaggacaga ccaaacatca tggttctgtg
      361 ttcattaggt tgttctgtcc attgctgaca taatccgctc cacttcaacg taacaccgca
      421 cgaagatttc tattgttcct gaaggcatat tcaaatcgtt ttcgttaccg cttgcaggca
      481 tcatgacaga acactacttc ctataaacgc tacacaggct cctgagatta ataatgcgga
      541 tctctacgat aatgggagat tttcccgact gtctcgttcg cttctcagtg gataacagcc
      601 agcttctctg tttaacagac aaaaacagca tatccactca gttccacatt tccatataaa
      661 ggccaaagca tttattctca ggataattgt ttcagcatcg caaccgcatc agactccggc
      721 atcgcaaact gcacccggtg ccgggcagcc acatccagcg caaaaacctt cgtgtagact
      781 tcctttgaac tgatggactt atgtcccatc aggctttgca gaactttcag cggtatacca
      841 gcatacagca tgtgcatcgc ataggaatgg cggaacgtat gtggtgtgac cggaacagag
      901 aacgtcacac cgtcagcagc agcggcggca accgcctccc caatccaggt cctgaccgtt
      961 ctgtccgtca cttcccagat ccgcgctttc tctgtccttc ctgtgcgacg gttacgccgc
     1021 tccatgggta ttttcagtgt tgccaccatc gtctgcagct ggctgacgta ccaggagtca
     1081 gagagcggaa ccagccggtg agtctgctga ccggcgggca ttctccccgc cgtcctggca
     1141 gctttttcgg tccgttgttt cagggtcgca agctgcacaa acggatacgg aggcgcaagc
     1201 gaaaaatccc cccgcgtcag cgccagtgct tcattaatgc gtgctccggt gttccacagt
     1261 gtggccagca gcatcttgcg gtgcagattc gggacgtaat ggagcagggc actcacttcc
     1321 ggagccagca gatattttgg cagttcatca tggaccatcg acatctggcg aagtgccaga
     1381 gctgccggat aatcaatggc aacaggcagc gatgcaggct gtccggcaga atacactgcc
     1441 ggtaccatga ctgcagactg gctgtgtata acggagcctg acatttatat tccccagaac
     1501 atcaggttaa tggcgttttt gatgtcattt tcgcggtggc tgagatcagc cacttcttcc
     1561 ccgataacgg agaccggcac actggccata tcggtggtca tcatgcgcca gctttcatcc
     1621 ccgatatgca ccaccgggta aagttcacgg gagactttat ctgacagcag acgtgcactg
     1681 gccaggggga tcaccatccg tcgcccgggc gtgtcaataa tatcactctg tacatccaca
     1741 aacagacgat aacggctctc tcttttatag gtgtaaacct taaactgcat ttcaccagtc
     1801 cctgttctcg tcagcaaaag agccgttcat ttcaataaac cgggcgacct cagccatccc
     1861 ttcctgattt tccgctttcc agcgttcggc acgcagacga cgggcttcat tctgcatggt
     1921 tgtgcttacc agaccggaga tattgacatc atatgccttg agcaactgat agctgtcgct
     1981 gtcaactgtc actgtaatac gctgcttcat agcacacctc tttttgacat acttcgggta
     2041 tacatatcag tatatattct tatgccgcaa aaatcagcgc gcaaatacgc atactattat
     2101 ctggctttta gtaagcctta tgtattttac ctttcgttat gttaaccaat aaaaattaaa
     2161 atctgcctta taaaaacaaa gcgtaattac cgcattcccg tttcgtatgg atccgacttt
     2221 atttgaataa acgactttcc ggaagtgtac cacctgagaa aatcatcata tcgctgtttt
     2281 ccgcccctgc gcggcgcatc acagtccaga gatacaacac aacatatagt gccatgggca
     2341 actgaatgag gcactgcatg ctgtgctgac gggatgtaac gctgtgcgga gggagaggaa
     2401 gatccgtaac caccagaaac cggatctgac caggaaaaga gaaagagtgt atgtaagttc
     2461 tgtactgtat gtatgaattg tattgtatgt attgtgtatt tttcataccg ggcatacgat
     2521 ggcgccattc cctgagaaac cggagccaga ccatgacatc cctctccccg gacaccgtcc
     2581 gccgcattga agacgccgcc gccgccctga tcgccgccgg caccccgaac ccgaccaacg
     2641 agcaggtccg tcagcacctc ggcggcggct ccctgtccca catctccccg gtgatgcgcg
     2701 cgttccgcgc ccgccagcgc gagcaggcca cgcccctgcc gccggagctg gcgcagctgc
     2761 tgaccggcca gctggggctg ctgtggcagg cggccgtgaa acaggctgaa gccggggccc
     2821 tggcggcgcg cgagcaggcc gacgacgaca ttgcccgggc cgacaaggag cgggatgagg
     2881 cgctggcgaa cgtggccgcg ctggaaagcg agctggcggt gctgcgcgag gtggtggccg
     2941 aacgtgaccg cctgctgcag gaggtacgcg agctacgcgc ggaggcgctg ccgctgcgcg
     3001 agcaggtggc ccgcctgacc gccaccggcg aacacctggc ggcgcagctg caggacacga
     3061 aggcggagct gaaggaagcc cgggaggacg gccgccagct tcagaccgaa ctgctggctc
     3121 tggcccgcca ggacgggaag gtgaagaaat gagacaggaa agagtattac gtataaaggc
     3181 atttcgacgg gcactggagc ttggtggcgg tgaacgggct gacgggtgtc gtcattttcc
     3241 ccgctggaaa acggaactga ggaatttccc gcacagcagc tgcgatttag ccagcaatac
     3301 tctggcgtac tacctcaaag aacgagagag atgccatccg tgcatcattt ttatggaagg
     3361 gaacgctggc tttcatgaag cggaaaactc aacagttcac gggcatgtca tcgtattgct
     3421 ggacggagag tacatcgatt tgacgctgga tcaattcgac gaatatccgg actacattcc
     3481 ttacgaacct gtcgaatctg acgggcagat tggtaagctg ttgcgtaata tcatgaagca
     3541 tgaggacccg gtaaaaaccc gccagattga tctcgatggt ggcgaagagc tgtatgcctg
     3601 gttacgcgat accgccgacg aagtgctggc agcagatccg gaatggcagg cccgggaacg
     3661 gtcgatagaa gaagcaaggg aagccgctat tacggtgttc ccgtttctgt ctgacgtgca
     3721 aaaaactgag tgcgaacagg gcccggacag tcaggaggct gccagatgaa gatttatcat
     3781 tacactgacc tgaacggcct gaagggcatc atcgaatcag gctcactgtg ggcgacgcac
     3841 ttttcctttc tcaatgacag taatgagctg actcacggca tgaactgcct tgagaatgcc
     3901 ctgcagtacc tgagggacga ctttaacccg aaaaccctta agttcatcga acaggcaccc
     3961 ttataagcgg ttgtgtatgc tgtttccctg gaatgattta tccacatatc cggaggctcg
     4021 atccagtaaa cagatccatg aatgatcaac aaaggatcca ttaaagatcc ccataccgct
     4081 gcaaaccttg tcactcatgg gccgggacca cgatcacata agcagtggca tgttactgat
     4141 aaactgtaac atgctaatga taagctgtat tcagtaatcc atatactgaa gtaagttaat
     4201 gacataaact atggtcagta cgccagactc agctgttaaa tacaggctgc aggtttttct
     4261 tcagtcagtt agcggggctc tgacacacga tttgctgttt attcttttac tgtccacagg
     4321 caggaggctt tctggaaaac gaaaattcag acatcaaaaa actgttcggc gaggtggata
     4381 agtcgtccgg tgagctggtg acactgacac caaacaataa caacaccgta caacctgtgg
     4441 cgctgatgcg tctgggcgtt tttgtaccga cccttaaatc actgaagaac agtaaaaaaa
     4501 atacactgtc acgtactgat gccacggaag agctgacacg tctttccctg gcccgtgctg
     4561 agggattcga taaggttgag atcaccggcc cccgcctgga tatggataac gatttcaaga
     4621 cctgggtggg gatcattcat tcctttgccc gccataacgt aattggtgac aaagttgaac
     4681 tgccttttgt cgagtttgca aaactgtgtg gtataccttc aagccagtca tcacgcaggc
     4741 tgcgtgagcg catcagccct tccctgaaac gcattgccgg taccgtgatc tccttttccc
     4801 gcaccgatga gaagcacacc cgggaataca tcacccatct ggtacagtca gcctactacg
     4861 atactgaacg ggatattgtt cagttacagg ctgatccccg cctttttgaa ctgtaccagt
     4921 ttgacagaaa ggtccttctc cagcttaagg cgattaatgc cctgaagcga cgggagtccg
     4981 cccaggcact ctacaccttt atagagagcc tgccccggga tccggcaccg gtatcgctgg
     5041 cgcggctgcg tgcacgcctc aatctgaagt ctcctgtatt ttcccagaac cagacggtca
     5101 gacgggcaat ggagcagctg cgcgagattg gatatcttga ttacacggag atccagcggg
     5161 ggcggacaaa actcttctgc attcactacc ggcgtccccg gttaaaagca ccgaatgatg
     5221 agagtaagga aaatccgttg ccaccttcac ctgcggaaaa agtcagtccg gagatggcgg
     5281 agaagcttgc cctgcttgag aaactgggca tcacgctgga tgacctggaa aaactcttca
     5341 aatcccgctg aacataaact gtagtcagtg aagagtgttc ctttactgac tacagcttat
     5401 attatcaggt gcagtgagtg gtctgctcac tgcagtttat attcagtttc ctgcagtgct
     5461 gcctgtagct gagctgtcat ctgccggtcc cttacgtgag tcaccccgta acctgatgct
     5521 gaggcattgc tcccttcata aaacatgact tactcactac agcttatata catgctccag
     5581 cttatgttat gtctgttctg ctgaccacag cttatataag gaagcgcgta tgccgtcacc
     5641 tcaggggagg cagtgtacgc aggatctccg cagcatcccg cccgtcacct gtgaaaggca
     5701 ctgccagcgt ggcagccata tccagcgcaa acactctggt ataaacctcc atcgaacgtg
     5761 gatccctgtg acctgccagg gcctggatga ctttccgggg ctggcggtga tagagcatat
     5821 gcatgatata gctgtgccgg aaggtgtgtg gtgtgaccgg aatcgaaaag tgtactccgt
     5881 cagcttcggc ccgtctgaca gcctgcttca gccagttgcg catggtttcg tcggtcacgg
     5941 cccataatgg ttcacgacga cggggccggg tggtgatcat ccagctttcc atctgcctga
     6001 catagcttat atccgtcagc ggaaccaggc ggacttcatc ttttggcggg cgtccgcgtc
     6061 gcgcacgcac tttttcggac aggatccgca caaacggcct tgctccatcc aggtcaaatg
     6121 attccggcgt cagcatccgg gcttcgccaa tacgcattcc ggtattccag agggtggcga
     6181 acagcatatg gtgacgctga tccggcatat agaaaagaag ggcactcact tccggggcca
     6241 gcaggtaggc cggtgtggcc cctgtggaag tggccattct tctcagggag agcgctgttg
     6301 caaaatccac ccccggcgca atgggtaaca ggaagacgcg ttctggtgaa ttctgcaggg
     6361 gaatgacatt gttcatgagt tatttttcac gtagcacacg cactgtttat ggatacaggt
     6421 attctgacct gaacgcccgg atccttccac gacaataaat cgcagcgtgc ggatcctttt
     6481 gtttgcttat cacgcattat tgcgggtaaa accaaatacg taaaggtacg ttttgcagca
     6541 gggatcccgt attaagccag tcgtggcgcg ggctgagggc tggtttgccg tttttgggag
     6601 aattggggta atgccaggac gctaatgatc tatcaatgga tggatgtaag tgacaattga
     6661 aaactgaaaa atgcgctatg tagattctga cagatttatg cacctgaaat acgttcaaaa
     6721 tccacatagc gtaactctga ttttccttac cagaaaaaag agggggaata accagtatct
     6781 atccccccta cacaattgca gaataata
//
LOCUS       DAZFAB010000172         6565 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZFAB010000172 DAZFAB010000000
VERSION     DAZFAB010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.172"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..1508)
                     /locus_tag="ACHJWA_004494"
     CDS             complement(<1..1508)
                     /locus_tag="ACHJWA_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscV/YscV/HrcV family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO4310951.1"
                     /translation="MFNKVLVGLRSHPELIILGLMVMIIAMLIIPLPTYLIDFLIGLN
                     LTLAILVFLGSFYVDRILSFSSFPSILLITTLFRLALAISTSRLILLEADAGEIITSF
                     GEFVIGDSLVVGFVIFSIVTIVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLR
                     AGIIDADLAKERRSVLERESQLYGSFDGAMKFIKGDAIANIIIIFVNIIGGLSVGVGQ
                     NGMDFSTALTVYTILTVGDGLVSQIPALLIAISAGFIVTRVNGDSDNMGQNIMSQLLS
                     NSFVIVVTCVLALSIGLLPGFPLLVFLCLAVILGIYFYFKFKKKGTEETVVEGDITVG
                     LEPYNQDDDISLGIINKLDQVITETVPLVLIMNSVQAKKYTEINLADRIRSQFFIEYG
                     IRIPGIVIREGEGLNDEDVILMLNEVRASQFKIYHDLVLLVEYSDEVVSTLIKKPVIV
                     NSNGEQYYWVTKSDAQKLTKIGCYTRTAMDEMYNHLSVCLAHNINEYFGIQET"
     gene            complement(1501..2646)
                     /locus_tag="ACHJWA_004495"
     CDS             complement(1501..2646)
                     /locus_tag="ACHJWA_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopN/LcrE/InvE/MxiC type III secretion system
                     gatekeeper"
                     /protein_id="HFO4310952.1"
                     /translation="MAIHVEHVGVLERAREVSRLEDIITEDNEDIEAEMPKMRDDPAG
                     KEARFLQATDEMSAALTQFMKKKIYEEQLANFLDGEEYVLEDQPIEKTDKVMEALKAA
                     TTHDYEVYSFAKKLFPDESDLVVVLRAILRKKQISENVRLNAEALLRKVNQETTKKFI
                     NSGINSALKAKLFGQALSLNPKLLRASYRQFLMAEDDAVDTYVEWIGSYGYQNRMLVT
                     KFIKETLFSDINALDASCSSLEFGMFLNKLSQLLSLQSAEALFLKTLMNNPIIKKFIS
                     AEDYWIFFLISLIKFPETAEELLNNALVTLPADANYKDKTLLLKAIYSGCTNLPFSLF
                     INNEQLLEIRECCKQAIKVTFAAELFDTQNCNKKQNKKPWKKVMFNV"
     gene            complement(2651..4354)
                     /gene="sctC"
                     /locus_tag="ACHJWA_004496"
     CDS             complement(2651..4354)
                     /gene="sctC"
                     /locus_tag="ACHJWA_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system outer membrane ring
                     subunit SctC"
                     /protein_id="HFO4310953.1"
                     /translation="MKIKLRITIILISVLCIFNGLLTPGAYAAAANGYVANKENLRSF
                     FETVSSYAGKPTIVSKLAMKKQISGNFDLTEPYALIERLSAQMGLIWYDDGKAIYIYD
                     SSEMRNALINLRKVSTNEFNNFLKKSGLYNSRYEIKGDGNGTFYVSGPPVYVDLVVNA
                     AKLMEQNSDGIEIGRNKVGIIHLVNTFVNDRTYELRGEKIVIPGMAKVLSTLLNNNIK
                     QSTGVNVLSEISSRQQLKNVSRMPPFPGAEEDDDLQVEKIISTAGAPETDDIQIIAYP
                     DTNSLLVKGTVSQVDFIEKLVATLDIPKRHIELSLWIIDIDKTDLEQLGADWSGTIKI
                     GSSLSASFNNSGSISTLDGTQFIATIQALAQKRRAAVVARPVVLTQENIPAIFDNNRT
                     FYTKLVGERTAELDEVTYGTMISVLPRFAARNQIELLLNIEDGNEINSDKTNVDDLPQ
                     VGRTLISTIARVPQGKSLLIGGYTRDTNTYESRKIPILGSIPFIGKLFGYEGTNANNI
                     VRVFLIEPREIDERMMNNANEAAVDARAITQQMAKNKEINDELLQKWIKTYLNREVVG
                     G"
     gene            complement(4351..5100)
                     /locus_tag="ACHJWA_004497"
     CDS             complement(4351..5100)
                     /locus_tag="ACHJWA_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4310954.1"
                     /translation="MIEEGLLLPMNVLLQGQKLTLLDPEMWFLRGNENKDVTLVITIG
                     NNHQKLMVVEDILLLIDRSQIEVTAGKVIYHPLRIDILSKLLAFIDESTGSVEREHHE
                     LFADALPFASEVVLFSKVASEAWFLATYLSSDNINEILFQHLRKTECYKLVRYLLSQS
                     LIQTSLYDLGELYGVSYSHFRRLCSYALGGKVKTELCGWRVARAVLEIIEGNSDMTTI
                     AHKYGYSSSSHFSAEVKSRLGKTPRELCKKL"
     gene            complement(5446..5625)
                     /locus_tag="ACHJWA_004498"
     CDS             complement(5446..5625)
                     /locus_tag="ACHJWA_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GhoT/OrtT family toxin"
                     /protein_id="HFO4310955.1"
                     /translation="MINKLLLAYLIGLVVTSTLIFIFSEEKVTYRLFAAIITGLTWPL
                     SLIPSIISLMIRKSD"
     gene            complement(5692..>6565)
                     /locus_tag="ACHJWA_004499"
     CDS             complement(5692..>6565)
                     /locus_tag="ACHJWA_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310956.1"
                     /translation="ALLIAYLDKANFYVMEDSGAWEEDARLNTSSVALVTSGLERLSN
                     LLSKKDSVFVSDLLREAKVNELDETLSTTRLNHLIDKGYERITLQLDLGGESPGYLEK
                     DKHYREADAALLNVIYPANLSKINTRRKEQVLKIVKKLAGPYGIKRYEKDNYQSANFW
                     FNDIKTDTDQNSHAKREKSFIPSTEAEWFFDSWYAKSAAIVYKESRKEEYLNDSVQFM
                     NRSLAQITGENMIGANGRSVPEMALPESYNYIHKSGTLHEAPSPIIPLNWSKASMTLM
                     LKEMSNLINDEGIK"
BASE COUNT         1952 a         1391 c         1067 g         2155 t
ORIGIN      
        1 gtttcttgta taccaaagta ctcattaata ttatgagcca gacagacaga taaatgatta
       61 tacatttcat ccatggccgt gcgagtataa cagccaattt ttgttaattt ttgagcatca
      121 cttttcgtaa cccaataata ttgttcacca ttactattta caatgacagg tttcttgatc
      181 aaagtcgaaa ccacttcatc tgaatattca accaacaaga caagatcatg ataaatttta
      241 aattgtgaag ctctgacttc atttagcatt aatatgacat cttcatcatt aagaccttcc
      301 ccctcacgaa tgacaatacc gggaatgcgg ataccatact caataaaaaa ctgactacga
      361 atcctatcag caagattgat ttcagtgtat tttttagcct gtacactatt cataatcaag
      421 accaagggta ccgtttcggt aataacctga tcaagtttat taatgatacc taatgagata
      481 tcatcatctt gattataggg ttccaaccca acggtaatat ctccctcaac aaccgtttct
      541 tcagtccctt tctttttgaa tttaaagtaa aagtaaattc ctaagattac tgccaaacat
      601 aaaaacacta gaaggggaaa acctggaagc aacccaatac ttagcgcgag gacacatgtc
      661 acaacaatga caaaagaatt acttaataac tgggacatta tattttgccc catattatca
      721 ctatcgccat ttacgcgcgt aacgataaat cctgcgctaa tagcaattaa taaagctgga
      781 atttgagaaa caagaccatc accgacagta agaattgtgt agacagtcaa cgctgttgag
      841 aaatccattc cattttggcc tactcctact gatagcccac cgataatatt aacaaagata
      901 ataataatgt ttgcaatagc atcacctttg ataaatttca tcgcaccatc aaaggaacca
      961 tacaactggc tctctcgttc taatacacta cgacgttctt ttgctaaatc tgcatcaatg
     1021 atcccggcgc gtagatcggc gtcaatgctc atttgtttac ctggcatacc gtcaagcgaa
     1081 aagcgggcag caacttcagc cacacgttcc gaacctttag taatcacgat aaactgaaca
     1141 atagtaacaa tggagaaaat aacaaaacca actaccaggc tatctccaat aacgaactca
     1201 ccaaaactcg ttataatttc tccagcatcc gcctcaagaa gtataagtcg actggtactg
     1261 atcgccagcg ccaaacgaaa taacgttgtg ataaggagga ttgatggaaa tgaagaaaag
     1321 cttaagatcc tatcaacata gaatgatcct aaaaatacaa gtattgccaa tgttaagtta
     1381 agcccaatca aaaagtcgat taaataggtg ggcaatggaa ttatgagcat ggcgataatc
     1441 atcaccatta atccaagaat tattaattct ggatgactcc ttaaccctac caaaactttg
     1501 ttaaacattg aacatcacct tcttccatgg ttttttattt tgttttttgt tacagttttg
     1561 tgtatcaaag agctctgctg caaatgttac ttttattgcc tgtttacaac actccctaat
     1621 ctctaataat tgttcattat taataaaaag tgaaaatgga agatttgtac atccactgta
     1681 aatcgccttc aggagtaacg ttttgtcttt ataattagca tctgcgggaa gggtcaccag
     1741 tgcattgttc aggagttcct ctgcagtctc aggaaatttt attaacgata tcagaaaaaa
     1801 tatccagtaa tcttctgcgc tgataaattt tttaattatt gggttattca ttaaagtttt
     1861 cagaaataaa gcctctgccg actgaagaga caatagttgt gacaacttat tgagaaacat
     1921 accaaattcc aatgatgaac aactcgcatc aagtgcattt atatctgaga aaagcgtctc
     1981 tttgataaat tttgtcacca acattctatt ttgatagcca taactaccaa tccattcgac
     2041 ataagtatct acagcatcgt cttctgccat taaaaattgc cgataactcg cccttagtaa
     2101 ttttggattt aatgacagcg cctggccaaa taactttgct tttaatgccg agttaattcc
     2161 gctattaatg aattttttag tagtctcttg attaactttt ctaagtaatg cttcagcgtt
     2221 tagccggaca ttttctgata tttgtttttt acgtaaaatt gcacgtaaca caactaccag
     2281 atcgctttca tctgggaata attttttagc aaagctatac acttcatagt catgggtagt
     2341 tgcagcctta agcgcctcca taactttgtc ggttttttct attggctggt cttccaggac
     2401 atattcttct ccgtccagaa aattggccag ttgttcttcg taaatttttt tcttcataaa
     2461 ctgcgttaac gctgctgaca tctcgtcagt agcttgtaag aaccgagctt ctttacctgc
     2521 gggatcatcc ctcattttag gcatttcagc ttcaatgtct tcattatctt ctgtgataat
     2581 gtcctctaag cgagaaactt ccctggctct ttctaataca ccgacatgct ctacatgaat
     2641 cgccatttca ctatcctccg acgacttcac gattcaggta agtttttatc catttctgca
     2701 gtaattcatc gttgatttct ttattttttg ccatttgctg tgtaatcgct ctggcatcaa
     2761 ccgcagcctc attcgcatta ttcatcatgc gttcatcgat ctctcttggc tcaataagaa
     2821 agacacgaac gatgttattc gcatttgttc cttcataacc aaataattta ccaataaatg
     2881 gaatgctgcc taatattggg atcttgcgac tttcataggt attcgtatcg cgtgtatatc
     2941 ccccaatcaa aagacttttc ccttggggca cgcgtgcaat agtacttata agtgttctac
     3001 caacctgagg cagatcatca acattggtct tgtcggaatt gatttcattt ccatcttcaa
     3061 tattcaagag taattcaatt tgatttcgcg cagcaaatcg tggtagcacg ctaatcatcg
     3121 taccataggt tacttcatcc aattccgcag tacgctcgcc gactaattta gtatagaaag
     3181 tgcggttatt atcaaatata gcaggaatat tctcttgtgt taaaacgacg ggacgagcaa
     3241 caacggcggc tcttcttttt tgtgcaagtg cctgtattgt tgcaataaac tgtgtcccat
     3301 caagggtact aattgaacct gaattattaa atgatgcact taagctggat ccaattttta
     3361 tcgtgcccga ccagtcagca cctagctgtt ctagatcagt cttatcaata tctattatcc
     3421 ataaagataa ttcaatgtgt cgctttggaa tatcaagcgt agctactaac ttctcgataa
     3481 aatcaacctg agatactgtt cctttaacaa gtaaactgtt ggtatcagga tatgcaataa
     3541 tttgtatatc gtcagtctct ggcgctcctg ctgtcgatat tattttctct acttgtaggt
     3601 catcatcttc ttcagcccca ggaaaaggag gcatacgtga tacgtttttt agttgttgac
     3661 gacttgaaat ttcagaaagt acgttcaccc ctgtgctttg cttaatgtta ttatttaaca
     3721 acgtcgatag tacttttgcc atgccaggaa taactatctt ttcgccacgt aactcataag
     3781 tccggtcatt aacgaatgtg ttgaccagat gaattattcc tactttattt cgaccgatct
     3841 caatgccatc gctgttttgc tccattaatt tcgcagcatt aacgaccagg tcaacataaa
     3901 ccggtggccc cgatacatag aaagtaccat tgccgtcccc tttaatttca taacgagagt
     3961 tatacagacc cgattttttt aagaaattat taaactcatt cgtcgatacc tttctcagat
     4021 taatcaatgc attacgcatc tctgatgagt catagatata aatagctttg ccatcatcat
     4081 accaaataag ccccatctgt gccgacaggc gttcaatcaa ggcatagggt tctgttaaat
     4141 caaaatttcc gctgatctgt ttcttcatgg caagcttact aactattgta ggcttacccg
     4201 catatgatga aaccgtttca aaaaaactac gtagattttc tttatttgct acgtatccat
     4261 tcgctgctgc ggcatatgca ccaggagtca ataatccatt aaaaatgcat aagactgaaa
     4321 ttaatataat agtaatgcgt aattttattt tcataacttt ttacatagtt ctctcggtgt
     4381 ttttcctaac cggcttttta cttcggctga aaagtgtgat gaggacgagt aaccatattt
     4441 atgcgcaata gttgtcatat cactgtttcc ttcaatgatt tccaacacgg ctctggcaac
     4501 tctccagcca cataattccg ttttaacttt accacccaat gcataactac ataaacggcg
     4561 aaaatgtgaa tacgatacgc cgtaaagctc accaagatca tagagtgaag tctgtatgag
     4621 cgattgcgat aataaataac gaaccagttt atagcattct gtttttctta gatgctgaaa
     4681 caggatttca ttaatattat cgctgctcag ataagtggct aaaaaccagg cttcacttgc
     4741 aactttactg aatagcacaa cttctgatgc aaagggcaag gcatcagcaa acaattcatg
     4801 gtgttccctt tccacactcc ccgttgattc atctataaaa gctaataatt tactcaggat
     4861 atcaatgcga agtggatgat aaattacttt ccctgcagtt acctcaattt ggcttcggtc
     4921 aatgagcagt aatatatctt ccactaccat taatttttga tggttattac caatcgtaat
     4981 aacaagggtt acatccttgt tttcatttcc ccgtaagaac cacatttcag ggtccagtag
     5041 ggttaacttc tgtccttgaa gcaaaacatt cattggaagt aacagccctt cttcaatcat
     5101 gtttcatctc cttattccat attctcattc tccataaact tgctataaaa acatgattaa
     5161 aaagtgatga ttactattta taaaataatg attttgttat gagcgaatag ctatcagcca
     5221 cttacactag tacgtcatcc tgtccatata cagatttgta actgttgata tgttttactt
     5281 aaacacacct cgtattactt ataacacttg tttggtaact attttcaata caaatcatat
     5341 ttgttagttc ttcattaaca cactgcgagt gttttgttac tccccgcata tattttattc
     5401 tcttgttcga ttctattttt cttaatcgat catataattt tcttgttagt cggatttacg
     5461 aatcatgaga cttataatgg aaggaatgag actaagcggc caggttaaac ctgtaattat
     5521 tgcagcaaac aaccgatatg ttactttttc ttccgagaag ataaatatta aagtcgatgt
     5581 cacaaccaga cctatcaggt aagctaacag taatttattg atcactttta cctccccttt
     5641 cgatttctct ttgagcggtc cttttaatcc aggaccgcct cattatgaaa attatttaat
     5701 tccctcatca ttgataaggt tggacatctc tttcaacatg agagtcattg aagctttcga
     5761 ccaatttagc ggaatgatag ggcttggcgc ttcatgcaaa gttcccgact tgtggatata
     5821 gttatagctt tcgggaagtg ccatttctgg tacgctcctg ccatttgcac caatcatatt
     5881 ctcgcctgtt atttgggcca atgatctatt cataaattgt actgagtcat tcaaatactc
     5941 ttcttttctt gattctttat aaacaattgc tgctgatttt gcataccatg agtcaaaaaa
     6001 ccattctgct tctgttgatg gaatgaagct cttttctctt tttgcatgcg aattttgatc
     6061 ggtatcggtt ttaatatcat tgaaccaaaa atttgccgac tgataattat ccttctcgta
     6121 tctttttata ccatacggac ctgctagttt tttaactatt tttagaactt gctcttttct
     6181 tctggtgttt atctttgaaa gatttgcagg ataaataaca ttaagtaaag ccgcatcggc
     6241 ttctctgtaa tgtttatctt tttccagata acctggggat tctcccccta aatcaagctg
     6301 taacgttatt ctttcataac ctttatctat gaggtggttc aacctcgttg ttgaaagagt
     6361 ttcatccagt tcattgactt tagcttcgcg tagtaaatca gagacaaaaa cagagtcttt
     6421 cttcgagagt aggtttgaca aacgttccag tccagaggtc accagtgcaa cggaagatgt
     6481 gtttaatcgg gcatcttcct cccacgctcc ggaatcttcc ataacataga aattggcttt
     6541 atccagataa gcaatcagca gcgca
//
LOCUS       DAZFAB010000173         6527 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZFAB010000173 DAZFAB010000000
VERSION     DAZFAB010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6527
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.173"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(14..475)
                     /locus_tag="ACHJWA_004500"
     CDS             complement(14..475)
                     /locus_tag="ACHJWA_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO4310957.1"
                     /translation="MTEQQIRQVCRQCMDRCRAGETWPPDLAEFVALISESGANPFGL
                     TVDAVMEEYRRWRNESWRYNGSDKYPWPQPVLYHICLEMRSKGIERQMTEGELKRLAE
                     RQLTKWAKHVSNGLSVPPVRRQLAAPKRPLGPTPIELLKQEYERRKAAGFV"
     gene            complement(468..1508)
                     /locus_tag="ACHJWA_004501"
     CDS             complement(468..1508)
                     /locus_tag="ACHJWA_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFO4310958.1"
                     /translation="MSIDALRWAKKVKTGSSSSKSVLTWLADMCGADLCAYPSVSALA
                     EVTELNKKTVQDSLRHLMEIGLIVDTGERKGRTKQIVVYRLIGVEESVAEPEYTQKRV
                     SLKVGKIGAVNKNSTENGYVSAQKSPKNGTLCCMENNQRHPNFPSKTPKNGSRNPKEP
                     KDLNPTHNARESAPTSEQEVLSLQAAPHVFLDGLSEPIGKFPMTDSWYPSRDFRRRAA
                     LWGMALPETEFTPAELTAFRDYWAAEGKVFTQIQWEQKFARHVNHVRAQVKPVSKGVN
                     HAAAPGGTASRAVQEIRAAREQWERENGFISDGNGLEAVGTHGGGLFEPLDPEERGRT
                     FEALDCTDWRDD"
     gene            complement(1480..2031)
                     /locus_tag="ACHJWA_004502"
     CDS             complement(1480..2031)
                     /locus_tag="ACHJWA_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO4310959.1"
                     /translation="MKITPEQVCEALDAWVCRPGMTQEQATILITEAFWALKERPNID
                     VQRVTFNDGEVDQRALGVNRVKIFERWKAIDTRDKREKFTALIPAIMEAIRISDFRLY
                     REISDGKSITYMIAGLNKEYGDVVESGLLFADPAVVERETDELIEKAIAFKHAYRQQY
                     QYYFADKQISARGSYEYRCTTMG"
     gene            complement(2015..2242)
                     /locus_tag="ACHJWA_004503"
     CDS             complement(2015..2242)
                     /locus_tag="ACHJWA_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO4310960.1"
                     /translation="MFKSDVINFYGTKAKVAKAAGVDPSAVSQWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRAGRLNNENHP"
     gene            2320..2727
                     /locus_tag="ACHJWA_004504"
     CDS             2320..2727
                     /locus_tag="ACHJWA_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4310961.1"
                     /translation="MNEKTLGQRIRERRKQVGLSQNDLSKAAGVSGSSISLWESDHTA
                     PRGQNLHRLAEVLQCSPTWILFGDEDKTPDPPVALNSALDLSEDELEMLRLYRALPKS
                     EQQAQISELRARVENFNRLFTELLEARKRNKHQ"
     gene            2917..3072
                     /locus_tag="ACHJWA_004505"
     CDS             2917..3072
                     /locus_tag="ACHJWA_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO4310962.1"
                     /translation="MDTIDLGNSESLVCGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            3074..3202
                     /gene="ydfB"
                     /locus_tag="ACHJWA_004506"
     CDS             3074..3202
                     /gene="ydfB"
                     /locus_tag="ACHJWA_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfB"
                     /protein_id="HFO4310963.1"
                     /translation="MDFDTIMEKAYEEYFEGLADGEEALSFNEFKQALSSSAKSNG"
     gene            3232..3450
                     /gene="ydfC"
                     /locus_tag="ACHJWA_004507"
     CDS             3232..3450
                     /gene="ydfC"
                     /locus_tag="ACHJWA_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFO4310964.1"
                     /translation="MQKREPVIIAPDYTDDELYEWMRQKINAAQDLKWANEARAKQAE
                     KLSALEQDITNLEKAAALSIARMITYPR"
     gene            complement(3454..3618)
                     /locus_tag="ACHJWA_004508"
     CDS             complement(3454..3618)
                     /locus_tag="ACHJWA_004508"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310965.1"
                     /translation="MNQLPRRALSRYGLTFQGNILSVNCQCRMLTRVRRTHSTSPVEN
                     YLITNLSFVG"
     gene            4016..4204
                     /gene="dicB"
                     /locus_tag="ACHJWA_004509"
     CDS             4016..4204
                     /gene="dicB"
                     /locus_tag="ACHJWA_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO4310966.1"
                     /translation="METSLPNVNTSEGCFNIGILLSNREFTEDAIRMRKYEPYLLNDN
                     SILSRIALLELGIFGGQQ"
     gene            4201..4389
                     /locus_tag="ACHJWA_004510"
     CDS             4201..4389
                     /locus_tag="ACHJWA_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO4310967.1"
                     /translation="MSSAFALMMTVFLITGEPQNVITGIYASKESCHQARDEQKISSE
                     CLPLNKVSLYLNNEIPAG"
     gene            4482..>6527
                     /locus_tag="ACHJWA_004511"
     CDS             4482..>6527
                     /locus_tag="ACHJWA_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
                     /protein_id="HFO4310968.1"
                     /translation="MSTDKEEIALYYEAKNDKVRKRLGIKGGFYWRTAKKLSVAISRG
                     VVAMDDAGFDEEDFKKPVRVNLPIVNDLPPEGVFDTEFCNRYEKGGEDGITMIFIAPS
                     PSVQDKPASSDNTNVNGEDMAEIEDNMLLPISGQELPIRWLAQHGSEKPVTHVSREEL
                     QALHIARAEELPAVTALAISHNTKLLDPLEIRDLHKLVRDTDKVFPNPVNSSLGLMTA
                     FFEAYLDADYTDRGLLTKEWMKGNRVLRISRTPSGANAGGGILTDRGEGFVHDDASVE
                     RDVAAGVLARSMDIDIYNPHPAHAKRIEEIVSENKPPFSVFRDKFIAMPGHLDYSRAI
                     VVASVKEAPIGIEATPHRVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTKE
                     GKQDDEEKPQPSGAMADEQATTEAVEPDTTEHNQDTQSMDAQPQINSVDAKYQKLRAE
                     LHEARKNIPPQNPVDADKLLAASRGEFVEGISDPNDPKWIKGIQTRDSVYQNQPETEQ
                     NDQKAEQNSPNTQQNEPETKQPEPVVQQEPEKICTACGQRSGGNCPDCGAVMGDATYQ
                     ETFDDKNLVEVQEDDSEKMEGAEHPHKENAGSAQDHASDSETGETADPLITVNGHRII
                     TSTSRTCDHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTIDLETAGGVID
                     "
BASE COUNT         1749 a         1659 c         1523 g         1596 t
ORIGIN      
        1 attacttctc aaatcagaca aaaccagccg ctttccggcg ttcatactcc tgtttcagca
       61 actcaattgg cgttggtccc aacgggcgtt ttggtgccgc cagttgtcgc cgtactggcg
      121 gaacgctcag gccgttacta acatgctttg cccatttcgt cagctgccgt tctgcaagcc
      181 gttttaattc cccttcggtc atctggcgtt caatcccctt tgaacgcatc tcgaggcaaa
      241 tgtgatacag cacaggctga ggccacggat atttgtcgct tccgttatat cgccaggact
      301 cattacgcca gcggcgatac tcctccatca cagcatccac cgtcaggcca aatggattgg
      361 ccccgctttc cgaaatcagc gccacaaact cagccaggtc cggaggccat gtttcacccg
      421 cccggcagcg gtccatgcac tggcggcaga cctgccggat ttgctgctca gtcatcgcgc
      481 caatctgtgc aatccagagc ttcgaaggtg cggccccgtt cttctgggtc cagcggttcg
      541 aataaacctc ccccatgagt tcccacagct tccaggccgt ttccgtcgct gataaatccg
      601 ttttcacgtt cccactgctc acgtgctgcc cgaatttcct gaactgcccg tgatgcggtg
      661 ccacctggtg ctgctgcatg gtttaccccc ttgctgactg gtttaacctg cgccctgacg
      721 tgatttacgt gacgggcgaa tttctgctcc cactgaatct gcgtaaacac tttcccctcc
      781 gctgcccagt agtcccggaa tgcggtaagt tcagcaggtg taaattctgt ctccggcaaa
      841 gccatccccc acaacgcagc ccgtcgtcga aaatcccgtg acggatacca gctatcggtc
      901 atcggaaatt ttccgatggg ttcgctcagg ccatccagga atacatgggg ggctgcctgt
      961 aacgacaaaa cttcctgctc actggtcgga gcactctcgc gtgcgttatg tgtggggttt
     1021 agatctttgg gttcctttgg gttccgtgat ccgtttttgg gtgtctttga tggaaaattt
     1081 gggtgtcttt ggttattttc catgcaacaa agagttccgt ttttggggct cttttgtgct
     1141 gaaacataac cgttttcggt actgttttta ttaacagcac caattttacc cacctttaaa
     1201 gacacccgtt tttgggtgta ttcaggctcg gcaacacttt cttctacacc gataagtcgg
     1261 tacaccacaa tttgctttgt tctgcctttt ctctcaccgg tatcaacaat taacccaatc
     1321 tccatcaggt gtcgtaagct gtcctgcaca gtctttttgt tcagttccgt tacttctgcc
     1381 agtgcagata cagacgggta tgcacacaaa tcggcaccgc acatatcagc aagccaggtc
     1441 aatacagact tactggatga actgccggtt ttcacctttt tagcccatcg tagtgcatcg
     1501 atactcatac gaacccctgg cagatatttg tttatctgca aagtaatatt gatactgctg
     1561 acgatacgca tgcttgaaag caatagcttt ttctataagc tcgtcagtct cacgttccac
     1621 aacagctgga tccgcaaaaa gcagcccgga ctccaccaca tcgccatatt ctttgtttaa
     1681 tccggcgatc atgtacgtaa tgctttttcc atcactgatc tcacgataca acctgaaatc
     1741 actaattcgg atagcctcca taattgccgg aatcagcgcc gtgaattttt cccgcttatc
     1801 cctggtgtcg atagctttcc agcgttcgaa tatcttcacc cggttaacgc ccagcgcccg
     1861 ttgatcaacc tcgccatcat taaacgtgac gcgttgaaca tcgatgttcg ggcgttcttt
     1921 cagagcccag aatgcttccg tgattaatat cgtcgcctgc tcctgtgtca ttcctggtcg
     1981 acatacccag gcatccagag cctcacaaac ctgttcaggg gtgattttca ttgttcaacc
     2041 gccccgcccg ctttgcctta cgatattcgt cataaacttt ggggtcgtac tgaagttccc
     2101 cgccggatgc ctcttgcagg cgcatcgcgc gaccttcagg aaccagttcc ccccattgag
     2161 aaacagcaga tggatcaaca ccagcagctt tcgctacttt ggctttcgtc ccataaaaat
     2221 taattacgtc tgatttaaac atcacccctc caaagttgag ttttctcaat agtaatcact
     2281 caaggaatct caagtcaagg gtgattaaga tatctaaata tgaacgagaa aactttaggt
     2341 caacgaatta gagaaagacg caaacaggtt ggtttaagtc aaaacgattt aagcaaagcc
     2401 gctggcgtat ctggctcatc aatttcacta tgggaaagcg accatacagc cccgcgcggg
     2461 caaaatttgc atcgcctggc tgaggtattg caatgttcac caacttggat actgtttggt
     2521 gacgaggata aaacaccaga tccaccagtt gcactcaaca gcgccttaga cttatcggaa
     2581 gatgagttgg agatgttgcg attgtatcgc gcacttccaa aatcagagca gcaagcacaa
     2641 atcagcgaac tccgtgcccg cgttgagaat tttaatcgcc tattcaccga gctactagaa
     2701 gctcgcaaac gtaacaaaca tcagtaaccc ccttcacaaa ttttaaagcc ttacatttca
     2761 atgtattggc tttattttgc attaaatatt gagttttctc attaaaaata cttgaccaac
     2821 attcatgaga aaactaaatt accacccatc aagacaccgc acggtgttct cagcaaacag
     2881 ttccgctacc cggcgttaag gggaaatgag gtcagcatgg atactatcga tcttggcaac
     2941 agcgaatctc tggtatgtgg cgtgttcccc aaccaggacg gcacgttcac cgcgatgacg
     3001 tataccaaaa gcaaaacgtt taaaaccgaa aatggtgccc gtcgctggct ggaaagaaac
     3061 tcaggtgagt gatatggatt tcgacacaat catggaaaag gcttatgaag aatacttcga
     3121 aggtcttgcc gacggcgaag aagctctcag cttcaacgaa tttaaacagg cgctttccag
     3181 ctcggcaaaa tctaacggct gataagcgaa acagcaccgc gaggaatcag tatgcagaaa
     3241 cgagaacccg tcatcatcgc accagactat accgatgatg aactttatga gtggatgcgc
     3301 cagaaaatta atgcagcgca ggatctgaaa tgggccaatg aagccagggc taagcaggct
     3361 gaaaaactgt ccgctctgga gcaggatatc accaatctgg aaaaagcagc ggcattaagc
     3421 attgccagaa tgattacata cccgcgttaa tagctaacca acgaagctaa ggttggtaat
     3481 taagtagttc tccacgggtg aggtggagtg cgtgcgccgg acgcgggtga gcatccggca
     3541 ctgacagttt actgaaagga tatttccctg aaaagtcaga ccataacgcg aaagcgcacg
     3601 gcgaggtagc tggttcatag atagcctgtc gttaaatttt cgtcgaccgt gcgcttccgg
     3661 ttgtggcaac ccgcgaaatg gtgcggcggt aagtatggcg gggttatttc ttccccgttg
     3721 aggacaccgg gttgtcaggt tgaccatacg cttaagtgac aaccccgctg caacgccttc
     3781 tgttatcaat tttctggtga cgtttggcgg tatcagtttt actccgtgac tgctctgccg
     3841 ccctttttaa agtgaatttt gtgatgtggt gaatgcggct gagcgcacgc ggaacagtta
     3901 aagctaaaaa cagcgttatg ggtgattctg tattccggcg ttaattgtta actggttaac
     3961 gtcacctgga ggcaccaggc accacatcac aaaattcatt gttgaggacg cgataatgga
     4021 aacgtcacta ccaaacgtta atacgtctga agggtgtttt aatattggta ttctgctcag
     4081 taaccgggag tttactgaag acgccatcag gatgagaaaa tatgagcctt atcttctcaa
     4141 tgataattcc atactctcca gaattgccct tcttgaactt ggcattttcg gagggcagca
     4201 gtgagttcag cgtttgcact gatgatgacg gtttttctta taacaggtga gccacagaat
     4261 gtgattaccg gaatttatgc cagtaaagaa tcctgccatc aggcaagaga cgagcaaaaa
     4321 atttccagtg aatgtctccc gttaaacaaa gtatcgctgt acctgaataa cgaaataccg
     4381 gctggataac ccgccagccg tattaacgcc atacccgttg attaagcatg ccagcaatgg
     4441 cagggattcg tacaacctta aaatagttat gaggtttatc aatgagcact gataaagaag
     4501 aaattgcact gtattacgaa gccaaaaatg acaaagtcag aaaacgcctt gggattaaag
     4561 gcggttttta ctggcgcaca gcaaaaaaat tatcggttgc aatatcacgg ggtgttgtcg
     4621 caatggacga tgctggattt gacgaagagg atttcaaaaa acctgttcgc gtgaatttgc
     4681 ccattgttaa tgacctgccg cctgaaggtg tgttcgatac tgaattctgc aaccgctatg
     4741 aaaaaggcgg ggaagatggc atcacaatga tatttatagc gccttccccc tcagttcagg
     4801 acaaaccagc cagctctgac aataccaacg tcaatggcga agacatggct gagattgagg
     4861 ataatatgct cctgccgatt tccggtcagg aactgcccat tcgctggctt gcgcaacatg
     4921 gcagcgaaaa accggtaacg cacgtttcac gggaagaact tcaggcatta catatcgcac
     4981 gagctgaaga actgcctgct gttactgccc tggctatttc ccacaacaca aagctgctcg
     5041 acccgctgga gattcgcgac cttcacaaac tggtacgcga cacagacaaa gttttcccta
     5101 atcccgttaa ttccagtctg gggttaatga ctgctttttt cgaagcatac ctggacgctg
     5161 actataccga tcgaggtctg ctgacaaaag agtggatgaa aggaaatcgt gttttacgca
     5221 tcagccgcac gccatccggc gctaatgctg gcggaggaat tcttaccgat cgcggtgaag
     5281 gttttgtcca cgatgatgcg tcagtagaac gtgacgttgc cgctggcgtt ctggcccgtt
     5341 caatggacat cgatatttac aatccacatc cggcacacgc caaacgcatt gaagaaatcg
     5401 tttcagagaa taagccgccc ttttctgttt ttcgtgacaa attcatcgcc atgcctggtc
     5461 acctggatta ttcccgcgcg atagtggttg cgtccgtgaa agaagcacca attggtatcg
     5521 aggctactcc ccaccgtgtt accgaatatc tgaacaaagt actgaccgaa accgaccatg
     5581 ccaaccctga tccagaaatc gtggatattg cctgcggtcg ctcctctgct ccaatgccgc
     5641 agcgtgtaac aaaagaagga aaacaggatg atgaagaaaa accgcagcca tctggcgcaa
     5701 tggcagatga acaggcaacg actgaagcag tggaaccgga tacaactgaa cataatcagg
     5761 acacgcagtc gatggatgct cagccacaga taaattctgt tgatgcgaaa tatcagaaac
     5821 tgcgggcaga actccatgaa gcccggaaaa acattccgcc ccaaaatcct gtcgatgcag
     5881 acaaattact ggctgcctct cgcggagaat ttgttgaagg gattagcgac ccgaatgatc
     5941 cgaaatggat taaggggatc cagacccgcg attctgtgta ccagaatcag ccagaaacgg
     6001 aacagaacga ccagaaagcg gaacagaaca gcccaaatac gcaacaaaac gagccagaaa
     6061 cgaaacaacc tgaaccagta gtgcaacagg aaccggaaaa gatctgcacc gcctgcggtc
     6121 agaggagtgg cggcaactgc cctgattgtg gcgcggtgat gggcgacgca acataccagg
     6181 aaacattcga tgacaagaac ctggttgaag ttcaggaaga cgattcggag aaaatggaag
     6241 gcgctgaaca tccacacaag gagaatgctg gcagcgctca ggaccacgcc agcgatagtg
     6301 aaactggcga gacggcagat cccttaatta cggtgaacgg tcatcgcatt atcacatcca
     6361 ccagcaggac gtgtgaccat ctaatgatcg accttgaaac catgggaaaa aatcctgatg
     6421 ccccgattat ctcaataggt gcaatatttt tcgatccgca aaccggagat atgggaccgg
     6481 aatttagtaa gactatcgat ctggaaactg ctggcggagt cattgat
//
LOCUS       DAZFAB010000174         6454 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZFAB010000174 DAZFAB010000000
VERSION     DAZFAB010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6454
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.174"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(179..2212)
                     /gene="betT"
                     /locus_tag="ACHJWA_004512"
     CDS             complement(179..2212)
                     /gene="betT"
                     /locus_tag="ACHJWA_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HFO4310969.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            2341..2928
                     /gene="betI"
                     /locus_tag="ACHJWA_004513"
     CDS             2341..2928
                     /gene="betI"
                     /locus_tag="ACHJWA_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HFO4310970.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRHQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            2942..4414
                     /gene="betB"
                     /locus_tag="ACHJWA_004514"
     CDS             2942..4414
                     /gene="betB"
                     /locus_tag="ACHJWA_004514"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HFO4310971.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPSLEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCNAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGLDNGAWVAPTVFTDCSDEMTIVREEIFGPV
                     MSILTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            4428..6116
                     /gene="betA"
                     /locus_tag="ACHJWA_004515"
     CDS             4428..6116
                     /gene="betA"
                     /locus_tag="ACHJWA_004515"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HFO4310972.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAM
                     DLDNWAKEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDCKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHDLPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKMADMIRGKEALPRSTAGYFVANGMPVRAKKMSRDLN"
BASE COUNT         1591 a         1786 c         1766 g         1311 t
ORIGIN      
        1 tccattcagc taatacaaaa taaaatcgtt aattaataaa atataaaatc atgaagtgat
       61 acaagaggaa tttcaggata tgacatgaca atcatatata atatatgcat aattttttac
      121 agcgagtcaa tgattcccgc gccatatgca gacatggcgc ggttttatgc aataacaatc
      181 acgcgtccgg gaacatcacg ctatggcccg gcgcttcacg atggagatga ataaagttaa
      241 ggtgccgctc gtactggtca agaatatcgg tgatcacctg ctctttgctg tagtccatca
      301 ggtcgttgcc ctggctgcct tctaacagga aggtttccag ccggtagtag gtcgatttcc
      361 cgctacgtgc gcggtaggta aagcccggca ccgagtattg ctgcggccaa atctgataga
      421 caaagttttg ctcttcgccc atatgcacca acaaatccag atgccccaac tgttgtccct
      481 cttccggcgg caggcttttt agctccacgt acgcgccgcg caaccgcaac tcctgcgcca
      541 cttcttccat tgccgggtaa cagaccgtct ccatcatctg tttagtgtaa cgcgtgcccg
      601 gataattcat caggcgcgag agacgttttt tccagctcag gcgatcctga agccccagcg
      661 gtcgcggtgc ggtatcgcgg ttggcacttt cacggcggta atcttctacc ttcagagatt
      721 tatacaaccc cgccatcacg aagaagatca caaagctgaa cggcagcccc ataatcaccg
      781 tggtgttttg cagcgcggat atcccgttgg tcatcagcat gccgagcgtc agcaggccaa
      841 tcgccaccga ccagaagacg cgcagccagc cgggggcgtc gctgttgata tctttaagct
      901 gcgaggtgaa attccccagc accagcgccc cggagtccgc cgaggtcaca taaaacagca
      961 ggccagtaat ggtggcgacg gaggcgctaa aggtaaacgc cggatactgc gccagcaggc
     1021 tgtagaagcc gcgctccgga tggaccatcg cttcctcggc aaatgccgcg ccgccgtgga
     1081 tgatttcata cagcgcgcta ttgccgaaca ccgagagcca taacagcgtg aaggtaaacg
     1141 gaataatcaa cgtgcccagc acgaactggc gaatggtacg cccacgcgag atacgcgcca
     1201 ggaacaagcc gacaaacggc gaccatgcca cccaccatgc ccagaagaag agcgtccagt
     1261 tattcatcca ctcaaccggg cggtcgaagg caaaactgtt gagcgtcatg cccataaagc
     1321 gattcacata gtcgccaaca ttcagcacca gtgcattaag caggaacgaa gtgtcgccca
     1381 taaacaacac gaacaggatc aatcccagcg ccagcgcgac attaagctcc gataacacgc
     1441 gaatgccctt atcgacaccg gatgtcaccg agatagtggc gattatcacc gacaaggcga
     1501 tcagtgccgc ttttgccgcc atcgaatcgg gaatatcaaa cagtacgctc aagccatagt
     1561 taagctgcac cacaccgata ccgagcgtag tggcaatacc gaagatagtg ccgatcaccg
     1621 ctgcaatatc cactgagtga cctatcggcc cgttaatccg tttaccgaag atcgggtaca
     1681 gcgccgagcg gatggtgagc ggcaaattat aacgatagct aaagtatccg agcgccatgc
     1741 ccatcagcgc atacatcgac cagccggtta agccgtagtg aaacagcgtc cagaccatcg
     1801 cctgacgcgc ggcctcaatc gtctgtcccg cgccttccgg cggctgcata tactgcgtta
     1861 ccggttcggc tacggaaaag aacatcagat cgataccgat cccggcagca aacagcatcg
     1921 ccgcccaact cagcaggctg aattccggtt tggattgttc tggcccgagc ttcaccgaac
     1981 caaaacgcga acaagcgata cagaccacaa agacaatata gagcgttgcc gccagcagat
     2041 agtaccaacc gaaggtttta gaaacccagt ccagcgtgcg gccaatccac agggccgaga
     2101 agtcgcgaaa caggatcgtt gtcagggaaa acaacaaaat cagtccggcg gaggtgtaaa
     2161 acaccaccgg attgattttg tccttttccc tgctgtgtga aaggtctgtc atccagtatc
     2221 cccactgtta ttgttaccat ttaaaatcaa attcgtaaca attaagacac attttatatt
     2281 gaacgtccaa tcaataaccg ctttaataga taaacaacgc tgatgaatgg agtggcaaaa
     2341 atgcccaaat tggggatgca gtcgatccgg cgcagacaac tgatcgacgc cacactggaa
     2401 gcaataaatg aagtgggcat gcacgatgca acgatcgcgc agatcgcccg ccgtgcaggc
     2461 gtttctacgg ggatcatcag ccactatttc agggacaaaa atggtctgct ggaagcaacc
     2521 atgcgcgata tcaccagtca gctgcgtgac gcggttttga atcgattaca tgcacttccg
     2581 cagggcagtg cagagcagcg attacaggcg attgttggcg gaaacttcga tgaaacgcag
     2641 gtgagcagtg cggcgatgaa agcctggctg gcgttctggg ccagcagtat gcatcagccg
     2701 atgctctatc gtttacagca ggtcagcagc cgccgcttgc tgtcgaatct ggtgagcgag
     2761 tttcgccgcg aattgccgcg ccaccaggca caggaagcgg gctacggcct tgccgcgctg
     2821 attgacggat tatggctgcg cgcggcactg agcggcaaac cgctggataa acctctcgca
     2881 cactcgctga cccgtcactt tattactcag catttaccca ccgattaacc gaggagacgt
     2941 gatgtcccga atggcagaac agcagcttta tatacatggt ggttatacct ccgccaccag
     3001 cggtcgcacc ttcgagacca ttaacccggc caacggtaac gtgctggcga ccgtgcaggc
     3061 cgccgggcgc gaggatgtcg atcgcgccgt gaagagtgcc caacaggggc aaaaaatctg
     3121 ggcggcgatg accgctatgg aacgctcgcg tattctgcgt cgggccgtcg atattctgcg
     3181 cgagcgcaat gatgaactcg ccaaactgga aaccctcgac accggaaaag cgtattcgga
     3241 aacctcaacc gtcgatatcg ttaccggtgc ggacgtgctg gagtactacg ccgggctgat
     3301 cccgtcgctg gaaggcagcc agatcccgtt gcgtgagaca tcatttgtct acactcgccg
     3361 cgaaccgctg ggcgtggtgg cagggattgg cgcatggaac tacccgatcc agattgccct
     3421 gtggaaatcc gccccggcgc tggcggcggg taatgcgatg attttcaaac cgagcgaagt
     3481 cactccgctt accgcgttaa agctggctga aatttacagc gaagcgggcc tgccggacgg
     3541 tgtatttaac gtgttgccgg gcgtgggcgc ggagaccggg caatatctga ccgagcatcc
     3601 gggcattgcc aaagtgtcgt ttaccggcgg cgtcgccagc ggcaaaaaag tgatggctaa
     3661 ctcggcagcc tcttccctga aagaagtgac catggaactg ggcggtaaat caccgctgat
     3721 cgttttcgac gatgcggatc tcgatctcgc cgccgatatc gccatgatgg caaacttctt
     3781 cagctccggt caggtgtgta ccaatggcac ccgcgtcttc gttccggcga aatgcaacgc
     3841 cgcatttgag cagaaaattc tggcgcgcgt tgagcgcatt cgcgcgggcg acgttttcga
     3901 tccgcaaact aacttcggcc cgctggtcag cttcccgcat cgcgataacg tgctgcgcta
     3961 tatcgccaaa ggcaaagagg aaggcgcgcg cgtactgtgc ggcggcgatg tactgaaagg
     4021 cgatggcctc gataacggcg catgggttgc accgaccgtg ttcaccgatt gcagcgacga
     4081 gatgaccatc gtgcgtgaag agatcttcgg gccggtgatg tccattctga cctacgagtc
     4141 ggaagacgaa gtcattcgcc gcgccaatga taccgactac ggcctggcgg cgggtatcgt
     4201 gacggcggac ctgaaccgcg cgcatcgcgt cattcatcag ctggaagcgg gtatttgctg
     4261 gatcaacacc tggggcgaat ccccggcaga gatgcccgtt ggcggctaca aacactccgg
     4321 tattggccgc gagaacggcg tgatgacgct ccagagttac acccaggtga agtccatcca
     4381 ggttgagatg gctaaattcc agtccatatt ctaaccagga ggtttatttg caatttgact
     4441 acatcattat tggtgccggc tcagccggca acgttctcgc tacccgtctg actgaagatc
     4501 cgaatacctc cgtgctgctg cttgaagcgg gcggcccgga ctatcgcttt gacttccgca
     4561 cccagatgcc cgctgccctg gcattcccgc tacagggtaa acgctacaac tgggcttacg
     4621 agacggaacc tgaaccgttt atgaataacc gtcgcatgga gtgcggacgc ggtaaaggcc
     4681 tgggtggatc gtcgctgatc aacggcatgt gctatatccg tggcaacgcg atggatctcg
     4741 acaactgggc aaaagaaccc ggtctggaga actggagtta tctcgattgc ctgccctact
     4801 accgcaaggc cgagacccgc gacgtgggcg agaacgacta ccacggcggc gacggcccgg
     4861 tgagcgtcac cacctccaaa cccggcgtca atccgctgtt tgaagcgatg attgaagcgg
     4921 gcgtgcaggc gggctacccg cgcacggacg atctcaacgg ttatcagcag gaaggtttcg
     4981 gcccgatgga tcgcaccgtc acgccgcagg gccgccgcgc cagcaccgcg cgcggttatc
     5041 tcgatcaggc caaatcgcgc ccaaacctaa ccattcgtac tcacgccatg accgatcaca
     5101 ttatttttga ctgtaaacgc gcggtgggcg tcgagtggct ggaaggcgac agcaccattc
     5161 cgacccgcgc gacggcgaac aaagaagtgc tgttatgtgc aggcgcgatt gcctcaccgc
     5221 agatcctgca acgctctggc gtcggcaacg ctgaactgtt ggccgagttt gatattccgc
     5281 tggtgcatga tttacccggc gtcggtgaaa atcttcagga tcatctggag atgtatctgc
     5341 aatatgagtg caaagaaccg gtttccctct accctgccct gcagtggtgg aatcagccga
     5401 aaatcggtgc ggagtggctg tttggcggca ccggcgttgg tgccagcaac cactttgaag
     5461 caggcggatt tattcgcagc cgagaggaat ttgcgtggcc gaatattcag tatcacttcc
     5521 tgccggtagc gattaactat aacggctcga atgcagtgaa agagcacggc ttccagtgcc
     5581 acgtcggctc gatgcgctcg ccaagccgtg ggcatgtgcg gattaaatcc cgcgacccgc
     5641 accagcatcc ggcaattctg tttaactaca tgtcgcacga acaggactgg caggaattcc
     5701 gcgacgcaat tcgcatcacc cgcgagatca tgcatcaacc cgcgctggat cagtatcgtg
     5761 gccgcgaaat cagccccggc acggaatgtc agacggatga gcagctcgat gagtttgtgc
     5821 gtaaccacgc cgaaaccgcc ttccatccgt gcggtacctg caaaatgggc tacgacgaga
     5881 tgtccgtggt tgacggcgaa ggccgcgtgc atgggctgga aggcctgcgc gtggtggatg
     5941 cgtcaattat gccgcagatt atcaccggga atttgaacgc cacgacgatt atgattggcg
     6001 agaaaatggc ggatatgatt cgcgggaagg aagcgttgcc gaggagcacg gcgggatatt
     6061 ttgtggcaaa tgggatgcca gtaagagcga aaaaaatgag tcgtgatttg aactgactca
     6121 ggaaccgatc agattgagag ttaccgttcc aggtatgggg acagaatcgt tatataaaac
     6181 ataggggtaa ctctcatgct tcatagtgac aacctcgcca gcaaattcta agctgactcg
     6241 ttcaatctcg ctgatgaagg tactatgaat gcactgataa accgcaggtg aaatgccact
     6301 cagcttaaga attacgccgc tagtgttcct ggctatggtg acttgttcac tcccgctcac
     6361 ggttagcata tacgaaaagg gttcttgtaa cgaggacaaa aataagtcag accaaaacaa
     6421 cgtggagctt taaacacaat tcgtataatt tatt
//
LOCUS       DAZFAB010000175         6400 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZFAB010000175 DAZFAB010000000
VERSION     DAZFAB010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6400
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.175"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            1..355
                     /gene="ydjO"
                     /locus_tag="ACHJWA_004516"
                     /pseudo
     CDS             1..355
                     /gene="ydjO"
                     /locus_tag="ACHJWA_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="protein YdjO"
     gene            complement(359..1750)
                     /gene="tcyP"
                     /locus_tag="ACHJWA_004517"
     CDS             complement(359..1750)
                     /gene="tcyP"
                     /locus_tag="ACHJWA_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFO4310973.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHH"
     gene            complement(1883..2473)
                     /gene="ydjM"
                     /locus_tag="ACHJWA_004518"
     CDS             complement(1883..2473)
                     /gene="ydjM"
                     /locus_tag="ACHJWA_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO4310974.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2636..3304)
                     /gene="hxpB"
                     /locus_tag="ACHJWA_004519"
     CDS             complement(2636..3304)
                     /gene="hxpB"
                     /locus_tag="ACHJWA_004519"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFO4310975.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3451..3987
                     /gene="yniB"
                     /locus_tag="ACHJWA_004520"
     CDS             3451..3987
                     /gene="yniB"
                     /locus_tag="ACHJWA_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFO4310976.1"
                     /translation="MTYQQAGRIAVLKRIMGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGTRMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(4028..4888)
                     /gene="yniA"
                     /locus_tag="ACHJWA_004521"
     CDS             complement(4028..4888)
                     /gene="yniA"
                     /locus_tag="ACHJWA_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFO4310977.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4994..5284)
                     /gene="ghoS"
                     /locus_tag="ACHJWA_004522"
     CDS             complement(4994..5284)
                     /gene="ghoS"
                     /locus_tag="ACHJWA_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO4310978.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5385..6314)
                     /gene="pfkB"
                     /locus_tag="ACHJWA_004523"
     CDS             complement(5385..6314)
                     /gene="pfkB"
                     /locus_tag="ACHJWA_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFO4310979.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
BASE COUNT         1653 a         1591 c         1570 g         1586 t
ORIGIN      
        1 ctgtcgggag catttttgct gcgattcaaa aaaatgagag tctcctaaag tttccttcga
       61 taatatcaat gcttaatatc aatcttcttt ttaatccact aaatcttccg ggaatgggaa
      121 gtggtatact tgaagatatt atgagtattc cagatagttc acttagaaaa cgtctcgggt
      181 atgaggtttt aagcttctcg ctacaggcac attcattgag ccaggaatgt attgataagt
      241 tggacatttt ttcgctgacg atctgtttaa atatgagtca gtatgcatcg ccgcgatgga
      301 acatttaaaa agtaaggcaa cggccccttt acaaaacgga ccgttgccag cataagaatt
      361 aatggtgtgc cagttcggcg tcgtcttcgc tatccagaat ggctttatcg gtttgcttca
      421 gccactggct ggtcagcgtg ccagctgtca tcgagccact aacgtttagc gccgtacggc
      481 ccatgtcgat aagcggttca acggagatta acagcgccac cagggttact ggcaggccca
      541 tcgcaggcag cacaatcagt gcggcgaaag ttgcaccacc accgacaccg gcaacgcctg
      601 cggagctaac ggtaacaata ccgaccagcg tcgcaatcca catcgggtcc agcgggttaa
      661 tgccaaccgt aggcgcaacc atcaccgcca gcattgccgg atacaaaccg gcgcagccgt
      721 tctgaccaat ggtcgcaccg aaagaggcgg cgaaactggc gatggattca ggaacaccga
      781 gtcgacgcgt ttgtgcttcc acattcagtg ggatagacgc agcactggaa cggctggtaa
      841 aggcaaacgt cagcacaggc cataccttac ggaagtactt cagcggactc acgccattaa
      901 tgcccagcag aatgccatgc actgcaaaca taatcagcag accgaggtag gacgcgacaa
      961 cgaaacttcc cagtttgatg atgtcttgca ggttagaacc tgcaaccact ttggtcatta
     1021 gtgccagaac gccgtaaggg gtcaactgca tgaccaggcg aactagtttc atcacccagc
     1081 tttgcagggt atcgatagcg gttaagacgc gttcaccttt cggcgcatca tccttcagca
     1141 gtttcagcgc agctacaccg aggaatgcgg caaaaattac cacgctgata attgacgtcg
     1201 gattggctcc ggtcagatcg gcaaacgggt ttttcgggat aaaggacaag accagctgcg
     1261 gaacgctcag gtcagagact ttaccaacat agttactttc gatggcgttc agacgtgcag
     1321 tttctgcacc accctgaacc agaccttcag ccgtcaaacc aaacaggttg gttaccagca
     1381 caccgaccag cgccgcaatc agcgtggtaa acaaaagcgt accaatggtc agaaaactga
     1441 ttttgcctaa ctgagatgcg ttatgcagac gggcaaccgc gctcagaata gaggcgaaga
     1501 ctaacggcat aacgatcatt tgcagcagtt gaacatagcc gttaccaaca atgttaaacc
     1561 actgtacaga atctttaagt acctggctgt cagaaccata aatggtatgc agggcaaggc
     1621 caaaaaccac acccatcacc agacccacca gcactttttt cgccagactc cactgtttat
     1681 ggcgggtctg agccagcgca aacagcagta caacgaacac cacgatgttc gcaattaatg
     1741 gaaagttcat ccccgttctc ctgatcttat tatttctatc actgcgtagc agtcatagtg
     1801 ttggcgcaag attaacagaa ctgtgagctg gctcttatat tcaaatggaa ttgcttatta
     1861 cagaaaggcg atttttgcct ttttagtatt caacctgatg cttaataagc cgatgaaact
     1921 ggatctgcaa ggtattgatc atttgcgatg accaacgaac agcgctgttt tcgggtaatg
     1981 aatgaggcat ccataccgac cagacaaata atgccatgca gataaaacgt tccagttggt
     2041 tacccttttg gggaaccagg ataggcaagc ggaaacgcca gcggcatggc cagagcaggg
     2101 gaacaccggc gggtgtcagc atatcggcaa gtatgtggct taaataaccc agcaccattc
     2161 cttgtagcgc atcagccgga atgaaccagc tttcaggaac cttaaggtaa aaggttgcca
     2221 gcagcgcaaa taccgccagc agactgtggg taaaaccacg atgcccaaaa gcgcgggcga
     2281 tcggttttga tatccacttt aatcgctgcc caagaaacga ctttgggtga tcgatgtccg
     2341 gtaacaaaca cgtcaggatt gcggaaggga caatatgcca ccagtcaccc tgtgccagca
     2401 cgggcgtcag ctcggcattt ttggcaaata ccgcacaagc aatagaaaag agaaggtgac
     2461 cttccgccgt catgataaaa ctcactaaac tgtcgatacg tacagtatag gatttttata
     2521 cagtgcacgg aagtggtgac ggtgtaactc tttctcttaa attgacgatt aatccgtcag
     2581 ttgaatgcaa attcagataa agaaccaggg gcagcgtgat cactgcccct ggtcattaac
     2641 cgagaaggtc ttttgcggtg agttctgtca gcgatgagag tttgacgttt gctaatacaa
     2701 aacgtggatc attttgcgct tctggcgcag gaacgacgat ggaacgcatg cgggctgctt
     2761 tagaggcgat catgccattt accgaatcct ccagcgctac gcaggtcagt gggtcaacgc
     2821 ccagttttgc tgcgcagtcg agatatactt gcggatgcgg cttgctgtaa ggcagttttt
     2881 cggccgaggc gagggcatcg aaactgtcgc gtaagtcaaa catggtcaac actttttcca
     2941 gcatatgtag tggtgacgcg gaggccagtc ccaccaataa accttgttct ttgcataacg
     3001 caacggcttc gcgcacgcct ggtaataatg gacgtgtctc ttcaaccagt gaaatggcac
     3061 gggcaataac ccgttctact acttcctgac ggcttggccc attccacggt tgccgggcgt
     3121 accaaagatc gaccaccata tcgatgcgta aacctaaggt gtccggcagc tcgttacgac
     3181 gggagatatc cacccccagg cttgccatca catccagttc ggctcgatcc cataaaggtt
     3241 ctgagtcgat aagtaatcca tccatatcaa aaattgcagc aagaatctga cgcggggttg
     3301 acattacaac ctctccttta gagcattatg tgtaaggtat taagccgtgc ttatgattaa
     3361 gcgcctatca tatcgccttt aaagaatggg cgaaaacgga tatctacagg taaactaagt
     3421 gaaacgacgc gtcttaatca aggagaactc atgacgtatc aacaagctgg acgcattgct
     3481 gttttgaaaa ggattatggg ttgggtgatt tttatacctg cattgatctc tacattgatt
     3541 tctttgctga aatttatgaa tacccggcag gaaaaccagg aaggcattaa tgcggtcatg
     3601 ctcgacttta ctcatgtcat gatcgatatg atgcaggcga atacgccttt tttgaatctg
     3661 ttctggtata actccccgac gcccaatttt aatggtggcg tgaacgttat gttctgggtg
     3721 attttcatcc tgatttttgt tggactggcg ttgcaggatt ccggtacccg gatgagtcgc
     3781 caggcgcgct tcttgcggga aggtgttgaa gatcaactca ttctggaaaa agccaaaggg
     3841 gaagaggggc taacgcgtga acaaattgag tcacgtattg ttgttccgca ccatactatt
     3901 ttcctgcagt ttttctcact gtatatcctg ccggttattt gtattgctgc aggttatgtg
     3961 ttcttttctc tgcttgggtt tatttaatca aaaggccgca cggagcggcc attatcttca
     4021 acccatatca cgcagctaat aatctatcca gtgactgctg agcaataacc aaatgctgac
     4081 cgccaaataa tcttgcacga tttaacagcg tgtagagttg gtaaaccggt tgacgttcaa
     4141 ggaaatcggc aggtagcggg gatactgact gatagccgtc atagatttgt ggcggctgtt
     4201 cagtatgcag cggtaacatc gccaggtcgc actctcggtc accccagtag caggccgggt
     4261 cgaaaatgta cgggccatcc ggacccagcg cacagttgcc ggaccataaa tcgccgtgca
     4321 acagagaagg ctgcggttga tgtgaggcga gacgctgctg aatatgctcg acgatggcat
     4381 cgatattgcc gaaagcgatc cctttttctg ctgccagttc caactgccag ccaatccgtt
     4441 gttcagcaaa aaacgttgac cagcgacgtt gccaggtgtt gggctgtgga gttgtggaga
     4501 gcgagttatc aaaatcgagt ccaaattgtg gttggtcgct ccattgatgt aaacgcgcaa
     4561 tttgctgacc aagaataaat gcgctatgcg catccagcgg acgaggtggg agataatcca
     4621 tcaccagaaa actgtagtca cggtcagcgc caactgccca aaccttaggc acggtgacgg
     4681 ttttactacg cgacagtaac tccagttggt cggcttcggc ggtaaaaccg ggaagcagtt
     4741 ccctttcatc acatttgacg aaaaagtcat gtcctgcata gcgcaaatgc catgcggcat
     4801 ggacttctcc gccaggcagt tcattacgca gttcgatttc gccttcacct aactgctcgc
     4861 tcaaaagacg actgattgcc tgccacatga tgtctctccc atgttgtctg ccagatcata
     4921 aggttagcgc aagaatgcgg tgtaaaaaca cgagctaacg cacaccacag actgattatt
     4981 gcccaaaaac catttatttt ctgttgctat tccattcctc ccaggtcgtg atggtgattt
     5041 caggatcttt gccggttgca ctttgggtaa gccccgaaac cagttctcta atttcttctt
     5101 cactttgggt actgataagt ccaaaagtgt tagtccccag ctcatggata tttccctcat
     5161 catccgtcat ggtgagcaaa aaaccgtcgc gagtcaggta attattcaac tcgttaattt
     5221 cagtcaatgt atcctcatgc aacatgacgg ttatgacata acggacaaga tcgccactgg
     5281 ccatagttca cccctttgtt atcacatgac ttttgagcat agtcggagaa acgcgttgcc
     5341 gacaggttgg tgatgattcc cccaatgctg ggggaatgtt tttgttagcg ggaaaggtaa
     5401 gcgtaaattt tttgcgtatc gtcatgggag cacagacgtg ttccctgatt gagtgtggct
     5461 gcactccccg cagctacgcc aaaacgaacc atctcttcaa gagaggcatt ttctgccagt
     5521 ttcagtgtca tcgcgccgac catgctatca ccagcgccaa cggtactctg gcttttcacc
     5581 ggtggtggca ccacctgaat gcagttttca ctatcaacac ccagcgctcc ttgtggaccc
     5641 agggaaacga caacccgttt ggccttgccg ctattaacga tttcctgcgc ggctttgcgg
     5701 acatcgtctg gctgggtgag ttcgcgattc accagcgcac tgagttcttt ttggttaggc
     5761 ttaaccaact cgatgttacc aatcgccagt gctgcactta acgcttcgcc agaactgtcg
     5821 atgatgcagc ggatcccttg gttttgcgca gcggaaatca gttgggttaa tttttccagc
     5881 ttcacacctg gcggcaggct tccgcttatg accaggatgg ccccggattc aatttccaga
     5941 acttgctctt caagctggcg aaactcatct tcatttaatg ccgcgcctgg cataacaaaa
     6001 cgatactgct caccgcttgc ttccacatgt acgtgtaaat tctgccgggt ccagtctttg
     6061 gcttctacag tagcgacggg gacattttca tccgccaaca gtgaaaccag gtgttcgccg
     6121 gtcgcgccac ccgccgggaa gatcgctgtg gcactgcctc caagatgggc aatggcgcgg
     6181 gcgacgttga tgccgccgcc cccgggttcg aacaccggtg cggtacagcg cagttttcct
     6241 tcgggataaa tttgcggggt aattgttgcg ctatcgagag agggcgcaag tgtcaacgta
     6301 tagatacgta ccatcatttc ctcctatagg ctgatttcag tctggcaccg aatcagcgga
     6361 aagtgaagaa attaacaata tgatttattg gagctttatt
//
LOCUS       DAZFAB010000176         6353 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZFAB010000176 DAZFAB010000000
VERSION     DAZFAB010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.176"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(253..855)
                     /gene="fimB"
                     /locus_tag="ACHJWA_004524"
     CDS             complement(253..855)
                     /gene="fimB"
                     /locus_tag="ACHJWA_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HFO4310980.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
     gene            2311..3027
                     /gene="nanC"
                     /locus_tag="ACHJWA_004525"
     CDS             2311..3027
                     /gene="nanC"
                     /locus_tag="ACHJWA_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFO4310981.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            3047..4153
                     /gene="nanM"
                     /locus_tag="ACHJWA_004526"
     CDS             3047..4153
                     /gene="nanM"
                     /locus_tag="ACHJWA_004526"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFO4310982.1"
                     /translation="MNKTITALAILMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLETQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLISHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAVDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNRLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            4218..5198
                     /gene="nanS"
                     /locus_tag="ACHJWA_004527"
     CDS             4218..5198
                     /gene="nanS"
                     /locus_tag="ACHJWA_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO4310983.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQSYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALVKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEAIYGNYQNNILANIIFVDFQQQGARGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSSAARRGIISDRFVEAILQFWRER"
     gene            5321..6016
                     /locus_tag="ACHJWA_004528"
                     /pseudo
     CDS             5321..6016
                     /locus_tag="ACHJWA_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT         1896 a         1364 c         1349 g         1744 t
ORIGIN      
        1 ttgcttaaaa aataaaggaa aaagtgcaga aacaggagag tttaagtgat gtgtaaattt
       61 atgaatgatt ttgtttattt attttgaaga acataaacca ttgtattttg tatggatatg
      121 gttaacttaa tgtttattat gataattaag aatcatactt acaataacat caattaataa
      181 atggttaatc atttttgata tatcgtaaga ataatgtagt ttttaacacc atccctggta
      241 tctcaactat ctctataaaa cagcgtgacg ctgtcgtcct ctggctctat cccagatgcc
      301 gtaaaaacgc cctgcattgc tggcagtata ccagacagta tgacgaatat tgcgatgccc
      361 aagataatcc tggataagtc gcgtatctat tcccatattc gccaaagcaa aaccacacga
      421 atggcgtaac atgtgcggat gaatctccag tgacaacccg gcattaccac cggaagtcga
      481 gataatatgg taaaattgtt gccgagaaag cggattcccc ttacgcgata aaaataccca
      541 ctcgctctca gcatgcggat aagaagtacg gatactcaac cagtttttta aagcctgaac
      601 ttctttgttc aatagcgggt gcgttgttga aaagcctttt tttaatcgat ggatatatat
      661 acactttgcc ttaagatcaa tatccgaaat cctcaatcga caaatttcac tcgcccggaa
      721 accatgaata aaacaaagca aagtcagaca ataattacgt gctgcatgag gcccggtatt
      781 tgctgcttta aggagtgatt cgatttcact atgggtcagg aagttccttt ttttgttatc
      841 agccttattc ttcatcgttt ttcccttata attacagacg cgcactagct gtgctaggtt
      901 agcaataatc caacattttt atcctgatta tgtttataaa agcgaacgtt tgcttaataa
      961 ctaacgtaag tggaccagtt cttctgagtg aacttaaatg gagtagcaac agttaattat
     1021 aataacgttg ccatacaaac gctatgctct tgcatgctat gtacctttat atatttatca
     1081 atcggatacg aatctaataa taaccctcat cattaatgca actaatctta tctatatatc
     1141 atgtgatatg ttctgtaaca agtaaaatca acataaaaaa ttcagcaaaa tttaatattc
     1201 gttcaactag ccgtctcaca tatgacatta ttagtcaact ccatttgcca caaaatggct
     1261 atttcatgga acacagtaat ttcttagtgg ctattatcaa gctaattctt aggtccctag
     1321 cgattattcc tggctgattc gtcagcttac gccatgagat tgttacattc ctttttatcc
     1381 tggagatatg ggggaacgca ggagatgatt ttttcatcta cattcgttgg taaatatagc
     1441 tttggtcact acgacttaaa ccttcctcaa tcacagccat tcgccgggcg atacggcaaa
     1501 caaactaagt gaaacatcat cctgaaaaca ccaacatcaa taagcctctc cagatcgact
     1561 tcagaagtga ccagttacaa gccacaaacc aggaaacata tttcatgatg agaattatgc
     1621 tcagcctgat ggcgggtaaa cactgattta caccaagctc actgagttag cttgcaaagc
     1681 ttcccttgtt tagactctaa ataaattatt tataaaacaa atagttaaac gaagatcttt
     1741 agtttttgaa gaccaccgca agtgttcgtc tggcttcaca tggcatcttc ttcttagaaa
     1801 aagatcgaca tattttgtga cacgaattgc aaatctggtt ttgttgtatg gattgcgtga
     1861 ttttcgatct ggtataacag gtataaaggt gcaccacgat agacaatgag acagggcatc
     1921 ttgcaatcta tggcaaacat cacttcagtt ctttctcatc gggtgatgaa aacgcacttc
     1981 agcctgaaag gaatatgaaa atgagatcaa cagacactct attttatgac tctgggtaaa
     2041 atgaattgag taagtgatat agcttacgaa cattcaaatc aattaaacat cagaatagat
     2101 tttatactca ggtatttaat ctggctctct gtttatttaa ataatgtgga aagagatttt
     2161 tcacaggaga cattatacaa aaaatataaa atacagctac cggttgccaa agacactata
     2221 agcctggcaa aaaaatatta cacaacataa atgctaattg tttatgcggg ctttgtattg
     2281 ctttctgtat cctacaaatg agtgaaattt atgaaaaagg ctaaaatact ttctggcgta
     2341 ttattactgt gcttttcgtc accattaatt tctcaggctg cgacactgga cgtacgtggt
     2401 ggatatcgta gtggaagcca cgcctatgag actcgactca aagtcagtga gggatggcaa
     2461 aatggatggt gggcaagcat ggaaagtaat acctggaata ccattcatga taataaaaag
     2521 gaaaatgccg cactcaatga tgttcaggtt gaagttaatt acgcgattaa acttgatgat
     2581 caatggacgg tgcgcccggg aatgttaacg cattttagca gcaacggcac acgctacgga
     2641 ccctacgtaa aactgtcctg ggacgcgaca aaagatctta attttggcat tcgctatcgt
     2701 tacgactgga aagcttaccg acaacaagac ttatccggtg atatgtctcg tgataacgtt
     2761 catcgttggg atggatatgt cacttaccat attaatagtg atttcacctt cgcatggcaa
     2821 acgacgctat acagcaaaca gaacgattat cgctatgcaa accataagaa atgggcgacg
     2881 gaaaatgcat ttgttctaca ataccatatg acgcccgata ttacgccata catagaatat
     2941 gactacctgg accgtcaggg tgtttacaac ggcagagata atttatcgga aaacagttat
     3001 cgcattggtg tgtcatttaa actgtagtag acaggagaca gtcacaatga ataaaacaat
     3061 aacggcgctt gctatcctaa tggcttcatt tgccgcaaac gcgtctgtat taccggaaac
     3121 tcctgtacca tttaaaagtg gtaccggagc aattgataac gacactgtct acattggttt
     3181 aggtagcgca ggtacggcat ggtacaagct ggagacacag gccaaagata aaaaatggac
     3241 agcgttagct gcattccctg gtggaccaag agatcaagca acctcggcat ttattgatgg
     3301 caatctgtat gtgtttggcg gcattggcaa aaacagcgag ggcttgactc aggtatttaa
     3361 tgacgtacac aaatacaacc ccaaaaccaa tagctgggtt aaattgatat cgcacgcgcc
     3421 gatgggcatg gcgggccatg tgacttttgt acacaacggc aaggcttatg ttactggcgg
     3481 tgttaaccag aatatcttca atggctattt tgaagatctc aacgaagctg gaaaagattc
     3541 aaccgctgta gataaaatca atgcacacta ttttgacaaa aaagcagaag attatttctt
     3601 taataagttt ctgttgtctt ttgatccctc tacacagcaa tggagttacg ctggcgaatc
     3661 tccctggtac gggacggctg gtgcggcggt tgtgaataaa ggtgataaaa cctggcttat
     3721 taatggcgaa gccaaacccg gattgcgaac ggatgccgta tttgaacttg atttcaccgg
     3781 taataattta aaatggaata ggcttgctcc cgtctcatca ccagatggcg tcgctggcgg
     3841 ttttgcgggg ataagcaatg attctcttat atttgccgga ggggccggat tcaaaggttc
     3901 acgagaaaat taccaaaacg gtaagaacta tgcgcatgaa ggcctgaaaa aatcatatag
     3961 cactgatatt catctttggc ataacgggaa atgggataaa tcgggtgaat tatcgcaagg
     4021 tcgggcctac ggagtatcat tgccctggaa taatagtcta ttgattattg gcggtgaaac
     4081 tgcaggcggc aaagcggtga cggattcagt tttgatctct gtgaaggata ataaagtcac
     4141 agtacaaaac taacgcttca gggccccggt aaaggggccc tgctatcaat ttgtcatttc
     4201 aattacgcga aatttatatg aacgcaataa tatcgcctga ttattactat gttcttactg
     4261 ttgctggtca gtctaatgcc atggcgtatg gcgaaggact gccattaccg gacagggaag
     4321 atgcgcctca tcccagaatt aaacaattag cgagatttgc gcatacgcat cccggaggcc
     4381 cgtcatgtca ctttaacgac attattccac tgactcactg cccacacgat gttcaggata
     4441 tgcagagtta tcaccatcct ctggcaacga atcatcaaac acagtacggc accgttggcc
     4501 aggcactgca tattgcacgg aaattactgc ccttcattcc tgataatgca gggattctca
     4561 tcgttccgtg ttgccgtggc ggatcggctt ttaccgcagg cagcgagggg acatattcag
     4621 aacggcacgg agccagccat gatgcttgtc gttggggaac ggatactccg ctataccagg
     4681 atttagtcag cagaacgcga gccgcactgg taaaaaatcc gcagaacaaa ttcctcggcg
     4741 tatgctggat gcaaggcgaa tttgacttaa tgaccagtga ctacgcgtca catcctcaac
     4801 actttaatca tatggttgaa gcctttcgta gggatctaaa acaataccat tctcagctta
     4861 ataatattac tgacgcgccg tggttttgcg gcgataccac ctggtactgg aaagaaaatt
     4921 tccctcatgc gtatgaagct atttatggca attatcaaaa taatatttta gctaatatta
     4981 ttttcgtcga cttccagcaa caaggtgcaa gagggctgac gaacgcgcct gatgaagatc
     5041 cggacgattt aagcacggga tattacggtt cagcgtaccg gtcaccggag aactggacga
     5101 cggcactgcg aagcagtcat ttcagctcag cagcccgtcg ggggattatt tctgacaggt
     5161 ttgtagaagc aattttgcag ttttggcgcg aaaggtgagc gttcattaat ttgatttatt
     5221 cgcatccatc agcgctttta actcctccgg cgtgcggcac tataacgact tccacaacaa
     5281 ggagccattt tcttatacca ctttcagtga ccatccgctt atgccaccac cctctacgca
     5341 actgtttgca gagcaccttc ccaccgagtg gatacagcac ttcctgacgc tttctgctca
     5401 tgcgaccgtt cgtcgtctta acctgagcgt ggatggcgaa gcggggatga acctgctggc
     5461 gcccgcagcg ctgtcaccca ggcgcgtcag cgcgtggggg ccgccccggt ggaatggctc
     5521 ttccgccaga ccacacagac atggggcgcg gaacgttacc tgaaggatga ctggcacggc
     5581 ctgcaacttt ttgccattga tggcgcacag ttcaggacac ctgatgaacc cgagctgcgt
     5641 gaatattatg gctctgccaa cacatccact gagcggcaga gcgcctaccc cgtcatgcgt
     5701 ctggtggcgt taatgaatct gggaatcaca ttctactgaa tgccatgacc gcgcctaccg
     5761 gcggagcgat acggcgtgag gcaacaaaag cggcgtcaga gatcagcttt aagttcgcgt
     5821 tccagtttat cgtgacagaa atgatagtgc tgggaaacac ggcgtcaccg gggactatcc
     5881 cgaaacgact ggagcatctg cgggggctct ggaagtggtg ttcataacaa aacgcccccg
     5941 accatcaagg gcgagggcgg ttaagatatc aaaaacccgt tatcctgtga aacacagtgc
     6001 cgccccgctt aagtgaacgg cattgtgttt ctgagggacc ttaattttcc ctgattctct
     6061 gacgttcctt accattgata tttccttcat tcctcaccag agaaataaaa aatcactata
     6121 ctcatcaaaa tatgagatta cctttattca ggtgatcacc ttactatttt ttacggaaaa
     6181 taaattatga ataacaagaa atcactgaca aaccacctgt aaaatgtcaa tcattttagt
     6241 tcaattgcct ttgtcatgat tatcttttta tcgtcctgac gatactatta acaattgcaa
     6301 tttttatacc cctcctgaac aaatagaaaa gaactaatat attcgtttat aat
//
LOCUS       DAZFAB010000177         6163 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZFAB010000177 DAZFAB010000000
VERSION     DAZFAB010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6163
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.177"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..446)
                     /locus_tag="ACHJWA_004529"
     CDS             complement(<1..446)
                     /locus_tag="ACHJWA_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4310984.1"
                     /translation="MWNLLRRTRKNQKSGRDVREAGWTSLFQAVAEPFSGAWQQGVKA
                     DPEAVLSFHAVFACISLISQDIAKMRLRLMQTDAHGIRRETRRGDIARLCRRPNAQQN
                     RIQFFELWLNAKLRHGNTVVLKIRNARGQIKELRILDWSRVEPLVA"
     gene            complement(392..613)
                     /locus_tag="ACHJWA_004530"
     CDS             complement(392..613)
                     /locus_tag="ACHJWA_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310985.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVRRPA
                     GQKKKRDAEHGECGTFYGEPEKTRNQDVT"
     gene            complement(658..2595)
                     /locus_tag="ACHJWA_004531"
     CDS             complement(658..2595)
                     /locus_tag="ACHJWA_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO4310986.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSAIPFLWQHDHSRPVGQCTVRRVSEGLEITATLVKPVPDMPSQLAARLDEVWAAIK
                     TGLVRGLSVGFRPHEYTFLDGGGLHFLRWELMEVSAVTVPANAECTIRTIKSYDRPFS
                     AASGNRKPVVKIASSAGAAAQSTTVFHKEKTIMNIGEQIKSFENKRAALAASLEEVMT
                     KAAEEGRTLDVEEEEHYDNTAAEIRQVDAHLKRLRELEAGKAATAQPVKQAGNGNVAA
                     VASAPVIRVEQKLDKGIGFARFAKSLAAAKGVRSEALEVARRQYPDDSRLHHVLKSAV
                     GAGTTTDPQWAGSLSEYQEYAQDFIDYLRPQTIIGRFGQGGIPALRQVPFNIRVHAQV
                     SGGAAGWVGEGKAKPLTKFDFESITFSHAKVSAIAVLTEELIRFSSPAADALVRNALA
                     EAVVARLDTDFVDPKKAAVADVSPASITHDVKGTASTGNPDADAEAAFGQFVAANLQP
                     TGAVWLMSSTNALALSMRKNALGQKEYPDMTLLGGSFQGLPVIVSQYVGDQLVLVNAP
                     DIYLADDGGVAVDMSREASLEMQSEPGGDSTTPSPVELVSMFQTGSVAIRAERWINWR
                     RRRTAAVAVITGVNYGSASGG"
     gene            complement(2659..4320)
                     /locus_tag="ACHJWA_004532"
     CDS             complement(2659..4320)
                     /locus_tag="ACHJWA_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO4310987.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPAPIYPEQAAHALAIFKQLRI
                     VDAPGSPTFGESCAQWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVESGAHLLMENLAM
                     VNPNLGYSVDEAFLYREYRKAREAGEEAFRGFMSKHANVEIGLALRSDRWAGADFWEQ
                     QGRRVSLDDILQRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWAHETAVV
                     RRKSEASRFQDLVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLDHIGIDPSGVGQIL
                     DSLAEAGIPDGIVVGISQGWKLGGAIKTTERKLAEGVLVHGDQPLMAWCVGNARVEPK
                     GNAILITKQASGRGKIDPLMALFNAVSLMSLNPEPKKKEYAVFFI"
     gene            complement(4317..4880)
                     /locus_tag="ACHJWA_004533"
     CDS             complement(4317..4880)
                     /locus_tag="ACHJWA_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO4310988.1"
                     /translation="MLTTQKRKFALALMSGKNKTASAIAAGYSAKTARVKGSQLAKDP
                     EVLAFIARKQCETVEVDEVPVYRQKKSEQEDKPRRREAAAIPQPDETNPEMPPPVVIS
                     PGIEYMEDGLPDPVKAMGRLLVENINTDPRLALDAAYKLAQFTHHKKGDAGKKSAKGD
                     AAKKAANRFAVPPPPRLVVNNDNEGNG"
     gene            complement(5171..5536)
                     /locus_tag="ACHJWA_004534"
     CDS             complement(5171..5536)
                     /locus_tag="ACHJWA_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO4310989.1"
                     /translation="MAVLRTLPGRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLR
                     DAGQCCLCGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSAREAAGGMPD
                     PTLPEVSGGHGRADDIIGL"
     gene            5578..5805
                     /locus_tag="ACHJWA_004535"
     CDS             5578..5805
                     /locus_tag="ACHJWA_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO4310990.1"
                     /translation="MSSKNRPRRTTTRNIRFPNQMIEQINIALDQKGSENFSAWVIES
                     CRRELAADIKYARQLTIKKNDTQYALRWLFI"
BASE COUNT         1340 a         1940 c         1535 g         1348 t
ORIGIN      
        1 tccgccacca gaggttcaac ccggctccag tccagaatac gcagttcttt gatctgcccc
       61 cgggcattac ggattttcag caccaccgta ttgccatgac gcagtttggc gttcagccac
      121 agttcaaaaa actggatgcg gttctgctgg gcgttgggac gacgacagag gcgggcaata
      181 tccccccggc gcgtttccct gcgtatccca tgcgcatccg tctgcataag acgcagccgc
      241 attttggcga tatcctggga tatcagcgaa atacatgcaa acaccgcatg aaaggagagg
      301 acggcttcag gatcggcttt cacgccctgc tgccaggcgc cggaaaaggg ctcagccacc
      361 gcctgaaaca ggctggtcca gcccgcctct cttacgtcac gtcctgattt ctggtttttt
      421 cgggttcgcc gtaaaaggtt ccacattcgc catgctccgc atcacgtttc tttttctgac
      481 ctgccggacg tcgcaccgtg atgtactccg cctttcccag gcgaaccagc acctccgcac
      541 acggctgtgc cacatcacgg atatccccgg cccgggcatc atgcgtgccc tgcagatatc
      601 ggatctttgc cataacctgt tacgggaggc gcacgcctcc cgtcctcctt atcagactca
      661 gccgccggac gcactgccgt agttcactcc ggtgatcacc gccaccgccg cagtacggcg
      721 acgacgccag ttgatccagc gctccgcacg gatggccacg ctgcctgtct ggaacatgga
      781 aaccagctcc accggggacg gcgtggtact gtcgccgccc ggctcagact gcatttccag
      841 tgatgcctcg cgggacatat ccactgccac gccgccgtca tccgccagat aaatatccgg
      901 ggcattcacc agcaccagct ggtcacccac gtactgggag acaatcaccg gcagcccctg
      961 gaaggagcca cccagcaggg tcatgtccgg gtattccttc tgacccagcg catttttacg
     1021 catggacagt gccagggcat tggtgctgga catcagccag accgcaccgg tgggctgcag
     1081 gtttgctgcc acaaactgtc caaacgcagc ctcggcatcc gcatccgggt taccggttga
     1141 tgccgtgccc ttcacatcat gggtgatgga cgccggggag acatctgcca ctgcggcttt
     1201 tttcgggtcc acaaagtctg tatccagacg cgccaccacc gcttccgcca gcgcattacg
     1261 gaccagtgca tcagcagccg gactggaaaa acggatcaat tcttccgtca gtaccgcaat
     1321 ggccgacacc ttcgcatgac tgaaggtgat ggattcaaaa tcaaacttcg tcaggggttt
     1381 tgccttaccc tcacccaccc agccggcagc accgccggac acctgggcgt gcacacggat
     1441 attgaatggc acctgacgaa gtgcagggat cccgccctga ccaaatcgcc cgataatggt
     1501 ctgcggacgc aggtaatcaa taaagtcctg tgcgtattcc tgatattcag acaggctgcc
     1561 tgcccactgc ggatccgtgg tggtccccgc gcccactgcc gatttcagga catgatgcag
     1621 acgactgtca tccggatact gacgacgggc cacttccagg gcttcagatc ggacgccttt
     1681 agccgcagcc agcgatttgg caaagcgggc gaagccaatc cccttatcca gtttctgctc
     1741 cacacggatc accggcgcag aagccaccgc ggccacattc ccgttaccgg cctgtttcac
     1801 cggctgcgcc gtggcggcct taccggcttc cagttcacgc aggcgcttca ggtgcgcatc
     1861 cacctgacgg atttccgctg cggtgttgtc gtaatgctct tcctcctcca catccagcgt
     1921 gcgcccttcc tctgcggctt tggtcatgac ctcctcaagg gaggctgcca gcgctgcacg
     1981 cttgttttca aaacttttaa tctgttcgcc aatattcatt atggtctttt ccttatgaaa
     2041 aacggttgtt gactgtgccg cagcgccggc agaagatgcg attttcacca ccggtttccg
     2101 gttgccggac gcggcagaaa acgggcggtc gtaagattta atggtccgga tggtgcattc
     2161 cgcattcgcg ggcacggtga cggcagacac ctccatcagt tcccagcgca gaaaatgcag
     2221 tccgcctccg tccagaaagg tgtattcatg gggacggaag cccacggaca gccccctgac
     2281 cagcccggtc ttaatggccg cccagacctc atccagccgg gcagccagtt gcgacggcat
     2341 atccggtacg ggcttcacca gtgttgccgt gatttccagc ccttcgctga cccggcgcac
     2401 cgtacactgc cccaccgggc gggaatggtc atgctgccag agaaacggga tcgcactgcc
     2461 aaactccgcg ccctccggct ccaggatgtc accatcccga tccggagaag gcgttgacgc
     2521 aatcccggtg atcacccgtt catcctcact gaaggatttc accgtcagca gggaacaggc
     2581 ccgtttaaga gtcacatcag cctcctgaaa ataaaaaaac cgccgcagcg gttcatgatg
     2641 gttacagtgt gaacagggtt atatgaaaaa aaccgcatat tctttctttt tcggttccgg
     2701 gttaagggac atcagggaga ccgcattgaa cagcgccatc agcgggtcaa tttttccccg
     2761 tccactggcc tgtttggtga taagaatggc gttaccttta ggctccaccc gggcattgcc
     2821 gacacaccag gccatcaggg gctggtcacc atgcaccagc accccttcag ccagtttgcg
     2881 ctcggtggtt ttaatggccc cgcccagttt ccagccctgg cttatcccca ccacaattcc
     2941 gtcggggatc ccggcttccg ccagtgaatc cagaatctgc cccacccctg acgggtcaat
     3001 accgatatgg tccagtaact cagcctcatg aatgcgacgc acatattccg ccacttccgc
     3061 cgtgtcatcc ccgacacgcc ggacaatggt catatctcca caggcaacaa gatcctgaaa
     3121 ccgggacgcc tcgctcttcc gtcggaccac cgcggtttca tgcgcccagg catggcccca
     3181 gcccagccat tcgcgggttt ccctgtcacg gccaatcacg tacattccca gcagatcatc
     3241 caggcccccg ccgtcaatcc ccaccgtcac cacatcagcg cgctgcagga tatcgtccag
     3301 gctgacgcgc ctgccctgct gctcccagaa atccgcaccc gcccagcggt cagaacgcag
     3361 ggcaagacca atttccacat tggcatgttt tgacatgaag ccacgaaatg cttcctcacc
     3421 agcctcccgg gctttacggt actcccggta cagaaaagcc tcatccaccg aataaccgag
     3481 attcgggtta accatggcga ggttttccat cagcaggtga gccccgcttt ccaccatttc
     3541 aggaggatgc tcaaaaatca ccggcagaaa gtgcggatca tgaattttgc cgtcacggac
     3601 atcccgggcg tactgcagtt tctgtctgaa caccccggct ggcggctcat tcgactgggt
     3661 ggtcgtatac accacaaacc cttccgggcg ggaggcaagg ccgcctatgg cttcacgtaa
     3721 catgtcctcc gctttgtact gcttgccaaa taaccacagt tcatcaatca gcgtccccac
     3781 ggacttgata ccggataccg tattcggatc ggcagccacc accttcaggg tggtgtccgt
     3841 caccctgtgg gtgatggtcc ggatatgtgt ctgcacctga cagaggtcat ccagatcatc
     3901 gtcccgtcgt accatatccc tggcagggtt gaaggcgtta gccgccacct ccacggtcgg
     3961 ggccagaatg gtgtagcccg ccgcctgccg ccagttcagt aacagcgccg tcatcatgat
     4021 cccggcagcc agcgtggact tgctgttttt cttggggata aggataaaaa cttccttgat
     4081 atggcgtaca ccggtctgcg catcgtagga gccaaacagg gccgccacca ggtcaaacac
     4141 ccactgtgcg caggactccc cgaacgtcgg gctgcccggt gcatccacaa tccgcagttg
     4201 tttaaaaatc gccagggcat gtgcggcctg ctccggataa atcggagccg gaataatcga
     4261 cagccccttt ttcaggcgct ctgcccagtc cgggcaggcc gtgctccaca caggtatcat
     4321 ccgttgccct cattatcatt attcaccacc aggcggggtg gtggtggcac cgcaaaacgg
     4381 ttagccgctt ttttcgccgc gtcacctttt gccgattttt taccggcatc cccttttttg
     4441 tggtgcgtga actgcgccag cttataagcc gcatccagcg ccagcctggg gtcggtatta
     4501 atgttctcca ccagaagacg ccccatcgct ttcaccggat cgggaagacc gtcctccata
     4561 tactcaatac caggagatat caccacgggc ggtggcatct ccggatttgt ttcgtccggc
     4621 tgtggtattg cagccgcctc acggcgacgg ggtttatcct cctgctctga ttttttctgc
     4681 cggtaaacag gaacctcatc cacctccacc gtctcgcatt gtttacgggc tataaacgca
     4741 agcacctcag gatcttttgc cagctgcgag cctttaaccc tggcggtctt cgccgaataa
     4801 ccggcggcaa tggctgacgc tgttttgttt ttcccggaca tgagcgccag cgcaaatttt
     4861 cgtttttgcg ttgtcagcac agcctcctcc cgggtccaga acgcactcag ccgggtatgg
     4921 ttcagcccat ttttcccggc atctcatgcc gcaaatgtta actgctgcct ggttaacatt
     4981 tgctgaaaaa gccagttaac attttttccg cacaacaaac tgaataataa agataaaaac
     5041 cgcaaaaatg cccggacagc cagttaacat gttaactgcc ctgaaacggg aattttttct
     5101 ctgcgtgaga gggggcgcgg tgtccggagc gatcgttttt ttcgccggat gatccccccc
     5161 ccggggcggg tcacagtccg atgatatcgt ctgccctgcc atgacctccg gacacctccg
     5221 gcagcgtcgg gtccggcata ccacccgccg cttcacgagc agacttttgt cgatggcatt
     5281 cggtacagag cgtccagaga ttcgtctcct cattaccacc accgaactga agtgcaattc
     5341 ggtgatcgag ttcactgtca cagaggtcaa ccacacgacc acagagacag cactgcccgg
     5401 catccctgag ccagatatga cgcttgaggg aaacacgtgc actgccactg acacgacgct
     5461 gttcaccctt cagaatattc acccgccggg tgttcagagt tttgattctg cccggtaacg
     5521 tacgaagcac agccatgtaa aatcctcgcc atatagcttg tcaccagagg aaagaaaatg
     5581 tcatcgaaaa accggccccg cagaacaaca acccgcaaca tccgatttcc aaaccagatg
     5641 attgaacaaa ttaacatcgc tcttgaccag aaaggttcag aaaatttttc tgcgtgggtc
     5701 attgaatctt gccgccggga gctggcagca gacataaaat atgcccgtca gttgactata
     5761 aaaaagaatg atacacagta tgctctgcga tggctgttca tataactatt tctttatatt
     5821 gctgaattta taaaaactca cagacattag ctgtattaat tccgaattga aataatcagc
     5881 catatagaat aaaaataaag cataacaata ataatcttct acccaatcag tacattactg
     5941 ctgtgactcc aacacggcag tttttttatt gaacagattc cagtttcttc caccatcgca
     6001 ccggacgggc gaccatgagg ggagaacgcc gcgctccgtt tacgcggtaa accccggtgt
     6061 gtatcgtttt tgattatccc cgcacactct cgcagaggag tctccctgtc gggctgcggt
     6121 ctctgttaat gcaggaatac ggcgacgata cggcgcatca gca
//
LOCUS       DAZFAB010000178         6096 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZFAB010000178 DAZFAB010000000
VERSION     DAZFAB010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6096
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.178"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(7..201)
                     /locus_tag="ACHJWA_004536"
     CDS             complement(7..201)
                     /locus_tag="ACHJWA_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310991.1"
                     /translation="MRALCAVNGSHPENLRFWLKACPVVLRERFHQQCKCRFNWSTQQ
                     LCENMGLRRFGTMEERNRLQ"
     gene            complement(222..503)
                     /locus_tag="ACHJWA_004537"
     CDS             complement(222..503)
                     /locus_tag="ACHJWA_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4310992.1"
                     /translation="MRCGLLMAICLCLVSFSGYAATGKSDAQIKKEIIKESIESYPGN
                     CACPYNHARNGSRCGGRSAYSRNGGYDVVCYEGDVTDEMIRQWKQENAE"
     gene            complement(1042..1128)
                     /locus_tag="ACHJWA_004538"
     tRNA            complement(1042..1128)
                     /locus_tag="ACHJWA_004538"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1092..1094),aa:Leu,seq:taa)
     gene            complement(1141..1214)
                     /locus_tag="ACHJWA_004539"
     tRNA            complement(1141..1214)
                     /locus_tag="ACHJWA_004539"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1180..1182),aa:Cys,seq:gca)
     gene            complement(1268..1343)
                     /locus_tag="ACHJWA_004540"
     tRNA            complement(1268..1343)
                     /locus_tag="ACHJWA_004540"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1308..1310),aa:Gly,seq:gcc)
     gene            complement(1495..2043)
                     /gene="pgsA"
                     /locus_tag="ACHJWA_004541"
     CDS             complement(1495..2043)
                     /gene="pgsA"
                     /locus_tag="ACHJWA_004541"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFO4310993.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(2100..3932)
                     /gene="uvrC"
                     /locus_tag="ACHJWA_004542"
     CDS             complement(2100..3932)
                     /gene="uvrC"
                     /locus_tag="ACHJWA_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFO4310994.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(3929..4585)
                     /gene="uvrY"
                     /locus_tag="ACHJWA_004543"
     CDS             complement(3929..4585)
                     /gene="uvrY"
                     /locus_tag="ACHJWA_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFO4310995.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     ANAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            4727..4843
                     /locus_tag="ACHJWA_004544"
     CDS             4727..4843
                     /locus_tag="ACHJWA_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO4310996.1"
                     /translation="MVTPVTNGLPDRFTVSPATKCDKNYLYSAKMKISPLNM"
     gene            4881..5057
                     /gene="yecU"
                     /locus_tag="ACHJWA_004545"
     CDS             4881..5057
                     /gene="yecU"
                     /locus_tag="ACHJWA_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFO4310997.1"
                     /translation="MIKIFIGHYINAFNSTVDGTLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            5044..5268
                     /gene="yecF"
                     /locus_tag="ACHJWA_004546"
     CDS             5044..5268
                     /gene="yecF"
                     /locus_tag="ACHJWA_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFO4310998.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEQQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(5336..6058)
                     /gene="sdiA"
                     /locus_tag="ACHJWA_004547"
     CDS             complement(5336..6058)
                     /gene="sdiA"
                     /locus_tag="ACHJWA_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFO4310999.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMETAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFNE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSAREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILRWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
BASE COUNT         1635 a         1569 c         1347 g         1545 t
ORIGIN      
        1 gctatttcac tgaagcctgt tccgttcttc catagttcca aacctccgca accccatgtt
       61 ttcacataac tgttgcgttg accaattgaa tctacactta cattgttgat gaaatcgctc
      121 acgaaggacc accggacagg ctttcagcca gaagcgcaga ttttcgggat gtgaaccgtt
      181 aacggcacat aatgctcgca caacaaacct gttaaaagag attactcagc attctcctgc
      241 ttccattgcc ggatcatttc atcggtaaca tcaccctcat aacataccac gtcataccct
      301 ccattacggc tatatgcact gcgtccacca catctactac cattccttgc atgattataa
      361 ggacacgcac aattgcctgg ataagattca atggattctt taattatttc ttttttgatc
      421 tgagcatcag actttcctgt tgctgcatat ccactaaaag atactaaaca aaggcagatt
      481 gccattaata aaccacatcg cataccgata cactcaatta ctaaccaatc atagcccaca
      541 tcataaaatc gagatgagga gcacaatacc tttaacaaga aggtgtcata acgataatat
      601 gtcgtgcaat tcatcaaaaa aaagatctat atcaaagaga tagaactctt atcagtgctt
      661 cagacatcgg cattcgaggc agcagaaaaa cagtaaatct gacgaatttg ccttttccca
      721 agatgttgtc agattaaaaa gatagattag cgcaggtacc tgttggtgga ttgcaggagg
      781 aaaaataaag tcgagtggaa aaatcccaga aaaatttttg tttaacagag gtcctggttc
      841 actatgcgaa atacggagga gctgcagaag aattctgctt catccttaga gtttaaacag
      901 cacgtgcact accattgttt ctgcatatcg ccacaccatc gccactctac cgccattgca
      961 caaaatacaa atacgaaaaa accacccgaa ggtggtttca cgacactgct tattgctttg
     1021 attttattct tagctttccc atggtacccg gagcgggact tgaacccgca cagcgcgaac
     1081 gccgagggat tttaaatccc ttgtgtctac cgattccacc atccgggctc gggaagaaag
     1141 tggaggcgcg ttccggagtc gaaccggact agacggattt gcaatccgct acataaccgc
     1201 tttgttaacg cgccaaattc ttcaggcctt tcagccagac atccgctgac gccgatgtct
     1261 tttaaactgg agcgggaaac gagactcgaa ctcgcgaccc cgaccttggc aaggtcgtgc
     1321 tctaccaact gagctattcc cgcattcatc aagcaatcag ttaatcactt gattttatta
     1381 tcgtctggca atcagtgccg ccgttcgatg cgttgcattc tacttacctg gcgcgatgag
     1441 tcaacgatat ttttcaccac ttttgatcgt ttgctgaaaa ttgcgccgaa acgatcactg
     1501 atcaagcaaa tctgcacgcg cagcgctcaa atattgcaac attgaccaca gagtcagtac
     1561 cgcagccaca aagaaaagtg caataccggc gtactcaacc caaatgttcg gacgccacag
     1621 cagccatgcc aacgccacca tctgggcagt ggttttcact ttcccaatcc aggagacagc
     1681 cacgctactg cgtttaccca actccgccat ccattcgcgt agcgcagaaa taataatttc
     1741 acgggcgatc atcgttgccg ccggtaaggt cacccaccag ctgtggtaat gctcggttac
     1801 cagcaccatg gcgatagcca cgagaacttt atctgctaca gggtcaagga aagcaccaaa
     1861 ccgggtactc tggttccagc ggcgtgccag aaaaccatcg aaccagtcag tcaccgccgc
     1921 gacgcagaaa atgagcgcgg cggcaaacgg cgaccaagtg acaggcagat aaaagaccaa
     1981 tacaaagaat gggataagga tgacacggaa cagtgtaagc aacgtaggga tattaaattg
     2041 cataatgacg ggtaactatc tgttgtcagt aagattaccc ctatgttgct acagagacat
     2101 caatgtttca acgaccagaa gatcttttct gccagacctt gcgaaatacc cggcactttt
     2161 gcaatttcct cgacgctggc gttacgtaaa ccttgcaaac cgcccatata tttcaacaac
     2221 atttgccggc gttttggccc gacgccttca atggtttcca gggaactggt atttttgacc
     2281 ttcgcccgtt ttttacggtg cccgccaatc gcgtgatcgt gtgattcatc gcgaatatgc
     2341 tggataacat gcagcgcggg agaatctggc ggcaaactga atccctcacc ttccggctca
     2401 aagaacagcg tttccagccc agccttacga tctgctcctt tggcaacacc aagtagcagc
     2461 ggatgatttt tatcccatga gacatccagt tcggcgaaga catttttcgc ctgcgcaagc
     2521 tggcctttgc cgccgtcgat aagtatcaca tccgggatct tactgtcgtc gatggcttta
     2581 ccataacgcc gacgcagcac ctgattcatc gccgcataat catcgcccgg cgtgatgcca
     2641 gtaatgttat agcgccgata ctccgcacgc agcgggccgt tagcatcaaa caccacacag
     2701 gaagcgacgg tttgttcacc catggtatgg ctgatgtcaa agcactccat ccgcttcact
     2761 tccggcaatt tcaacacgct ggcaagtgct gtaagccgct ggtgaacggt agattgctgc
     2821 gaaagtttgc tggttaaggc cgtcgccgca ttggtgcgcg cgagtttcag gtaacgcgcc
     2881 ctatcgccac gaggtttggt ttgaacatta atcttgcgtc ccgccagttc tgaaagggaa
     2941 tcggcgagca gcgttttatc gctaagatta aaatcgagca ggatctcacc cggtaaggtg
     3001 cgcatctggc tgccttgtaa atagaactgg cctacgaagg tttctaccac ctcgctcagt
     3061 tccgtaccgc caggcacttt cgggaaatag ctgcggctgc cgagcacttt gccctgacga
     3121 atgaacaata cgtggacaca agccataccc gcatcgaacg ccacaccaat aacgtcgagg
     3181 tcgtcgccgg tattggaaac gaattgtttt tcggtgacgc gtcgcaccgc ctgaatttgg
     3241 tcgcgaatac gtgccgcttc ttcaaactcc agattctgac tggcagtttc catacggcta
     3301 atgagttgcg taagcacctg atcatctttg ccagacaaaa acaggcgcac atactcgacc
     3361 tgctgagcgt attcttcttc actcaccagt ccttcaacgc acggtcccag acagcgccct
     3421 atctgatatt gcagacacgg acgcgagcga ttgcgataaa cactattttc gcactggcga
     3481 atggggaaaa tcttttgcag tagcgccagt gtttcacgta cggcatagcc attcgggaac
     3541 gggccgaaat attcaccttt ggcatgcttt gcaccacgat gcatcgccag acgcgggtgg
     3601 gtatcgccac tcaggaagat aaaaggatat gatttatcat cgcgtagcaa aacgttgtaa
     3661 cgcggctgat agagtttgat gtagttgtgt tccagcaaca gcgcttcggt ttctgtatga
     3721 gtaaccgtta catcaatttg ctggatctgg gcgaccagcg cttcggtttt gcgcgaagcg
     3781 aggttgctac ggaaatagct ggaaagccgt tttttcaggt ctttcgcttt gccgacatag
     3841 ataaccgtac caccagcatc gtacatgcga taaacgccag gctggctggt tacggttttt
     3901 aaaaacgctt ttgcgtcaaa ctgatcactc actgacttga taatgtctcc gcattacaca
     3961 gaccatggcg aattgccagg tgagtcagct caacatcgcc atgaatgttt agtttactga
     4021 acatacgata gcggtagctg ttcaccgttt tcggactgag attgagctgt tctgagatct
     4081 cattgacctt ctggcccttc gtgatcatca gcataatctg caattcacgt tcagacaaac
     4141 tggcaaatgg gctttctgtt ttttctggtt cgatctggct taatgccatt tgttgagcga
     4201 tgtcagaagc aatgtaacgc tgcccagaat agacagaacg gatcgcactc acgacttcct
     4261 gcggagccgc gcctttgctg aggtagcccg cagcaccggc ctgcatgact ttcgctggta
     4321 aagggttttc tgtatggacg gtaagcatga tgatttttac atcagctgtg gaacgcgcga
     4381 ttttacgcgt cgcctcaaga ccgccaatgc ccggcatact catgtccatt agcaccacgt
     4441 caacggcatt tgcccggcac cacttaacgg cgtcttcacc gcacgatgcc tcaccgacga
     4501 cttttatacc ctttatatct tccagaatgc gtcgtatccc tgcgcgcacc agttcgtggt
     4561 catcaacaag tagaacgttg atcaaaggaa tatctccaga aatagggata acgctactga
     4621 tagttagtca ttcgtatatt agcggttttt attgcaactt tgaaacgtta aaaatggtgg
     4681 gtattcgatt tttctctcgt ttttgtcaat tcgattgtcc acagttatgg tcacgcccgt
     4741 caccaacggc ttaccggatc gtttcacagt aagccctgcg acgaaatgtg acaaaaatta
     4801 cctttattca gcaaaaatga aaatcagccc gctgaatatg taacattaat taacccgaac
     4861 aatacgggca aaaaacattc atgataaaaa tatttattgg tcattatatt aacgcattta
     4921 atagcaccgt tgacggcacg ctcaaaaaac aaccactgct atttttagca aagcttatgg
     4981 tatactccgc cgccttaaca tttttcaccg caaattttca ttgcaacatg acgaggaaaa
     5041 taaatgagta cgcctgattt ttctactgcc gagaataatc aagaactggc aaatgaagtc
     5101 tcctgcctga aagcgatgct gacgctgatg ctgcaggcga tgggacaagc tgacgcgggc
     5161 cgcgtgatgt taaagatgga acaacagctt gcgctgatcg aagacgaaac ccaggctgca
     5221 gtattttcca aaacggttaa gcaaattaaa caagcctacc gtcagtaata taaaaccggc
     5281 tgatagcgtg cctttcagcc ggtttttgca tctggcacgc aggacagaaa agagatcaaa
     5341 ttaagccagt agcggccgcg taacaggcaa cctgggtctt atttggtgca ttaatttttt
     5401 tctgcatgtt tttctggtgg aaattgaccg tattctcaga gattgacaaa atcatcgcta
     5461 tctctgctga tgtcttccct tccgccgtcc acctcagaat ttctttttcg cgcttgctga
     5521 aattcatctc tggcgtcatc actatttcat catttaaacg catcagagcc atcagacttt
     5581 cgcgcaccag taactgcatt tttaattgca gttcatcact aagaatgggt atttcgcgcg
     5641 cgctgcaacg ggaaaaggac aaaaagccca gcgcccggtt tggcagcatt aaatactgag
     5701 tgacaccgcg gcgtaaacca tgtgcgcgcg cggcttccca taacggctgt gcttcgttga
     5761 ataagtcatc attccacatt agatggccct gactaaagtt ttcagggttc agcaccggat
     5821 caattgcgag aaagtttttt gcctgataat aactaaccca cgcctcaggg taattggtgt
     5881 agaaagccac tttaggtcga gtgaatggta ccgggtggcg gacacataac gaatagtaat
     5941 cgtactccag ctgctgagcc tgaagctcaa tttcatggta gacctcttct gcggtctcca
     6001 tcctctgaaa acgcaacagc atcgtgcgac gccagctgaa aaaatccgta tcctgcatag
     6061 taaaccgcaa cgcccctgag agtgagtatc atttat
//
LOCUS       DAZFAB010000179         6095 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZFAB010000179 DAZFAB010000000
VERSION     DAZFAB010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6095
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.179"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            118..720
                     /gene="ais"
                     /locus_tag="ACHJWA_004548"
     CDS             118..720
                     /gene="ais"
                     /locus_tag="ACHJWA_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFO4311000.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLGRLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKDKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(759..1184)
                     /gene="nudI"
                     /locus_tag="ACHJWA_004549"
     CDS             complement(759..1184)
                     /gene="nudI"
                     /locus_tag="ACHJWA_004549"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFO4311001.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEPGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLAHYDLNVATRKTLRLKGLL"
     gene            1463..2005
                     /locus_tag="ACHJWA_004550"
     CDS             1463..2005
                     /locus_tag="ACHJWA_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFO4311002.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            2105..3307
                     /locus_tag="ACHJWA_004551"
     CDS             2105..3307
                     /locus_tag="ACHJWA_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFO4311003.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTLALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            3527..4309
                     /locus_tag="ACHJWA_004552"
     CDS             3527..4309
                     /locus_tag="ACHJWA_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFO4311004.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCNAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTKLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPATVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYADVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            4324..5524
                     /gene="rhmD"
                     /locus_tag="ACHJWA_004553"
                     /pseudo
     CDS             4324..5524
                     /gene="rhmD"
                     /locus_tag="ACHJWA_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050032 - L-rhamnonate dehydratase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
     gene            5581..>6095
                     /locus_tag="ACHJWA_004554"
     CDS             5581..>6095
                     /locus_tag="ACHJWA_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4311005.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMG"
BASE COUNT         1452 a         1457 c         1588 g         1598 t
ORIGIN      
        1 ggcattttgc cagcatcgta ggatttttaa tattacacca actgctaatt atcgccagaa
       61 atgtcgctca atttcacggt aattgtctgg ttgcgcttgt ctataggtgg agtttacgtg
      121 ttagcttttt gccgctcatc attgaagtca aaaaaatatt tcatcatttt actggcgctc
      181 gctgcaattg ccggactggg tactcatgcc gcctggagta gcaatggttt gccacgtatc
      241 gacaataaaa cgctgggcag actggcacag cagcacccgg ttgtcgtttt gtttcgccat
      301 gctgaacgtt gcgatcgttc aaccaatcaa tgcttgtcag ataaaacagg tattacggtt
      361 aaaggtaccc aggatgcccg tgaactgggc aacgctttta gtgctgatat ccctgatttc
      421 gatctttatt ccagtaatac cgtccggacc attcagtccg cgacctggtt ttcagcgggt
      481 aaaaaattga cggtagataa acgacttctt cagtgcggta atgagattta tagtgcgatt
      541 aaggacttac aaagcaaagc gcctgataaa aatatcgtta ttttcaccca taatcattgc
      601 ctgacatata ttgctaaaga taagcgtgat gcgacattta aacctgatta tctggatggt
      661 ttagtcatgc atgtggaaaa aggcaaagtt tatctggatg gggaattcgt taatcactaa
      721 attttcaatc tgacagccag taatggctgt catcattgtt acagaagacc tttcaaacgt
      781 aacgtttttc gggtggcgac attcaaatca tagtgcgcca gatcttcagg ttttacccac
      841 gcgtagtcct gaaactcttc gtttattttc acttctcggt tggcagaaac gcaatcaaaa
      901 atcaggtaaa tcatataaat ttcttccttg cgaccatctg catacgtctt ggtgcgaata
      961 tcatcgctga aggtccaggg cgtgatttct gtcaaaagca gctgttctcc cagttcttcg
     1021 cgaatttcgc ggcgtagagc ctcttcaatt cgttcgccgg gctccacgcc gccacccgaa
     1081 agcgcccatt gaccggggaa aacgccgcga tcgtcggcca ttttacacag caaataagca
     1141 ccatcatttt gaatcaaagg gcatacaata gtccgttgtc gcacaggggt attccttata
     1201 aataaaaaga aaccatgaga atacggtaac tcagaaatat gtgaaagtga cagtaatctc
     1261 ttttgcgatg ataactgcac ttattattat ttttgtgtgt atcggtaaac tgtgtttatt
     1321 ttttcggcgt ttaattattt aagaagtatt tagttacaat aagtgactag atcccttttt
     1381 tcgctcattg aatcctctgc gccaggcagt acaatcccgc agctttggga attttgatta
     1441 attgcttgtg ggaaagggga taatgaaaaa aattgcgcta gcaggtctgg ccggaatgct
     1501 gttggtttct gcatcggtca atgcaatgag catcagcggc caggcgggta aagaatacac
     1561 caatattggt gtcggttttg ggactgaatc gacgggcctg gctttaagcg gtaactggac
     1621 acataacgac gacgacggtg acgtcgcggg cgtggggctg gggttgaatc tgcctctcgg
     1681 gccgttaatg gcgaccgttg gcggaaaagg cgtttacacc aacccgaatt acggcgatga
     1741 aggctatgcc gcagcggtag gaggtggttt gcagtggaaa attggcaaca gcttccgttt
     1801 gtttggcgag tattactact ctccggattc actctccagc ggtattaaaa gttatgagga
     1861 agcgaatgct ggcgcgcgtt acaccattat gcgtccagtc agtattgagg cgggttatcg
     1921 ctacctgaat ctgtcgggta aagacggtaa ccgcgacaac gccgtggctg acggcccgta
     1981 tgttggggtt aacgccagtt tctgatccct tccggcgcgg cattctgtcg cgccattcag
     2041 tttcttacta cgccttatct ccctttgcgt tatagtgatt cgataacaaa agcaggagat
     2101 aaccatgtta aaagtggaaa tgctatccac cggggatgaa gtgttacacg ggcaaatcgt
     2161 tgacactaac gctgcctggc tggccgattt tttctttcat caggggttgc cattatctcg
     2221 ccgcaatacg gtgggggata accttgatga cttagtcacc attcttcgcg aacgtagtca
     2281 gcacgccgat gtgctgatcg ttaacggcgg gctgggaccg accagcgatg atttaagcgc
     2341 actcgccgct gcgacagcaa aaggtgaagg cctggtgctg catgaagcct ggctcaaaga
     2401 gatggaacgc tatttccacg aacgtggacg agtaatggca ccgagcaacc gtaaacaagc
     2461 ggagctgcct gccagtgctg aatttatcaa taacccggta ggcaccgcct gtggttttgc
     2521 cgtgcagctt aatcgttgcc tgatgttctt tactcccggc gtaccgtcag aatttaaggt
     2581 gatggtcgag cacgaaatcc tgccgcgtct gcgcgagcgt ttttctttac cacagccgcc
     2641 ggtttgtctg cgtttaacta cctttggtcg ttcggaaagc gatctggcac aaagcctgga
     2701 cactctacaa ctgccgccgg gcgtaacaat gggctatcgc tcctcaatgc cgatcatcga
     2761 actgaaactc accggaccgg caagcgagca acaggcgatg gaaaaactgt ggctggacgt
     2821 taaacgtgtt gccggacaga gcgtgatttt cgaaggcacc gaaggactgc ccgcgcagat
     2881 cagtcgcgaa ttgcaaaacc gccagttcag cctgacgttg agcgagcaat tcaccggtgg
     2941 tttattggct ttgcaacttt ctcgcgcagg tgctccactg ctggcgtgtg aagtggttcc
     3001 ttcacaggaa gaaaccctgg cgcaaactgc gcactggatt acagaacggc gagccaacca
     3061 ttttgccggg ctggcactgg ctgtttcggg tttcgagaac gagcatctca actttgcgct
     3121 agccacgcca gatggcactt tagctctgcg tgtgcgtttc agcactacac gctatagcct
     3181 ggctatccgt caggaagtgt gcgcaatgat ggcactgaat atgctacgcc gttggttaaa
     3241 cggccaggac attgccagtg agcatggctg gattgaggtt gttgagtcca tgaccttatc
     3301 cgtctgattc cgccactctg tgccggttta ctgcaccggc acattccccc tccggcaaaa
     3361 cttgtccaac ctgttgtact caataagaaa aaatccgtgc gctgattcac aaatttgtgc
     3421 gtaatttctc cagtccgttg gacgaggagc gggatttgat gcgcttcaca attttactcc
     3481 ccctgagcaa cgagactgat tccagtatct aagactggag tccagtatgt tggaatcaag
     3541 caaagttccc gccctgacac gggctattga tattctcaat ttgattgccc gcattggccc
     3601 ctgtaatgct gcgacgatca ttgacacatt ggggatccct aaaagcacgg cctatttgct
     3661 gcttaatgaa ctcaggcgtc agcgttttct tagccttgat caccaggaaa atttttgttt
     3721 gtggaccaag ctggtggagc tttccggtca tgcgttaagc aagatggatc tccgggaact
     3781 ggctcgaccg cgtttgacgc aactgatgga taccaccggg ctgttatgtc acctggggat
     3841 tattgataac ggcagcgctt attacatttt gaaagtggag tcatcagcca ctatcagcgt
     3901 gcgttcacat gaaggaaaaa gcctttcgct ttatcgctcc ggaataggta aatgcctgct
     3961 tgcctggcaa cctgcaactg tacagcaaag tattatcgaa gggctggtct gggagcaggc
     4021 gacacccaca acgattactc atccacagca acttcacgaa gaactggcgc gtattcgccg
     4081 tcagggctgg agctatgaca acggcgaaga ttacgccgat gtccgttgtg tcgctgcgcc
     4141 ggtgtttaac gccaataacg agcttaccgc tgcgatctcg gtggtcggta cccgcttaca
     4201 aatcaatgaa gaataccgtg attatctggc gggcaaggcc attgcctgcg ccagggatat
     4261 ttcacgtctg ttgggatgga aaagtccctt tgacttacaa gcctcataac aatggagaac
     4321 atcatgaccc taccaaaaat taaacaggtt cgcgcctggt ttactggcgg tgcgacagca
     4381 gaaaaaggcg ctggcggcgg tgattatcac gaccaggggg cgaatcactg gatagacgat
     4441 catattgcca ccccgatgag taaataccgc gattacgagc agttacgcca gtcatttggc
     4501 attaacgttc ttggcacgtt ggttgttgaa gtcgaagcag aaaacggcca gaccggattc
     4561 gctgtttcga cagccggtga aatgggctgt tttatcgtcg aaaaacacct taaccgtttc
     4621 attgagggta aatgtgtcag tgatatcaaa ctgatccatg atcaaatgct caatgccacc
     4681 ctgtattact ccggctctgg tggcctggtg atgaacacga tttcctgtgt cgatctggct
     4741 ctgtgggatc tgttcggcaa agtggtcggg cttccggttt ataaactttt aggcggcgct
     4801 gttcgtgatg agattcagtt ctacgccaca ggtgcgcgtc cggatctggc aaaagagatg
     4861 ggctttatcg gtggcaaaat gccgacgcac tgggggccac atgatggcga tgcggggatc
     4921 cgcaaagatg ccgctatggt cgcggatatg cgtgaaaaat gcggtgagga tttctggtta
     4981 atgctcgact gctggatgag tcaggacgtg aactatgcga ccaaactggc ccacgcttgc
     5041 gcgccctata acctgaaatg gagagtgtct gccgccacag cagtatgaag gttatcgcga
     5101 actgaaacgc aacgcgccag tcggaatgat ggtcaccagc ggtgagcacc acggcacact
     5161 gcaatctttt cgtacgcttt cagaaaccgg tatcgacatt atgcagccgg atgttggctg
     5221 gtgcggtggc ttaaccacgc tggtggaaat tgccgcaatc gccaaatcca gggggcaact
     5281 ggtggtgccg cacggttcgt ctgtttactc ccaccatgcg gtgatcacct tcactaatac
     5341 gccattcagc gaattcctga tgactagtcc ggattgttca acgatgcgtc cgcagtttga
     5401 cccgattctg ctcaatgagc cggttccggt gaatggtcgt attcataaat cagtgcttga
     5461 taaacccggt tttggcgtcg aactcaatcg tgactgcaat ttgaaacgcc cctacagcca
     5521 ctaatcactc cgggcgttgc tgtgacaacg gtaacgccca catattctaa ggagcttttt
     5581 atgagcaccg ctttgcttga cgccgtggtg aagaaaaacc gtgtgcgttt aattccgttt
     5641 atgttggcgc tgtatgtgct ggcgtttctc gaccgttcga atatcggttt tgccaaacag
     5701 acctaccaga ttgataccgg gttgagtaat gaagcttatg cgctgggagc aggcattttc
     5761 tttgtggtat atgcgtttct gggtgttccg gcgaatcttt tgatgcgcaa actgggggcc
     5821 agaacctgga ttggtacgac aacactgctg tggggatttc tttcggcagc catggcatgg
     5881 gccgatactg aagcgaaatt tctgatagtt cgcactctgc ttggtgctgc ggaggccgga
     5941 tttttccctg gtatgattta tctcacctcg caatggtttc cgcagcgtaa tcgcgccagc
     6001 attatggggc tgttctatat gggcgcaccg ctggcgttaa cactgggatc accgctttcc
     6061 ggcgcgctgc tggagatgca tggatttatg gggca
//
LOCUS       DAZFAB010000180         6071 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZFAB010000180 DAZFAB010000000
VERSION     DAZFAB010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6071
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.180"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..253
                     /locus_tag="ACHJWA_004555"
     CDS             <1..253
                     /locus_tag="ACHJWA_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO4311006.1"
                     /translation="SGAVIRGVFDDPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRG
                     DTLTIGEENFWIDRISTDDGGSCHLWLGRGVPPAVNRRR"
     gene            265..842
                     /locus_tag="ACHJWA_004556"
                     /pseudo
     CDS             265..842
                     /locus_tag="ACHJWA_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            839..1234
                     /locus_tag="ACHJWA_004557"
     CDS             839..1234
                     /locus_tag="ACHJWA_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO4311007.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVLDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            1242..1982
                     /locus_tag="ACHJWA_004558"
     CDS             1242..1982
                     /locus_tag="ACHJWA_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4311008.1"
                     /translation="MPTPNPLAPVKGAGTTLWVYNGNGDPYANPLSDNDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWAATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASTSVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGEFAAVAEINVTAS"
     gene            1998..2420
                     /locus_tag="ACHJWA_004559"
     CDS             1998..2420
                     /locus_tag="ACHJWA_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO4311009.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPKKTQMPSMNEAVKQIEQEVLTTWPAEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            2402..2836
                     /locus_tag="ACHJWA_004560"
     CDS             2402..2836
                     /locus_tag="ACHJWA_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO4311010.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            2829..5378
                     /locus_tag="ACHJWA_004561"
     CDS             2829..5378
                     /locus_tag="ACHJWA_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO4311011.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSMSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGEEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQSQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRMLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAVLGDKVTYQEHLNALAQQADKFA
                     QQQRAKRAAIDAKNRGLTDRQAAREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGSWMAGLRSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGGASASGGTAIQAAAAKFHFAAGGFTGTGGK
                     YEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSMADSRSQASGTFE
                     QNNHVVINNDGTNGQIGPQALKAVYDVARKAAMDVVTGQMRDGGLFSGGGR"
     gene            5375..5704
                     /locus_tag="ACHJWA_004562"
     CDS             5375..5704
                     /locus_tag="ACHJWA_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4311012.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNADLKTY
                     SVTLSVPRWEATALESFLAEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            5704..>6071
                     /locus_tag="ACHJWA_004563"
     CDS             5704..>6071
                     /locus_tag="ACHJWA_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO4311013.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGN"
BASE COUNT         1409 a         1486 c         1988 g         1188 t
ORIGIN      
        1 gtccggcgca gtaatacgtg gtgtttttga tgaccctgaa aatatcagct atgccggaca
       61 gggcgtgcgt gttgaaggct ccagcccgtc cctgtttgtc cggactgatg atgtgcggca
      121 actgcggcgt ggagacacgc tgaccatcgg tgaggaaaac ttctggatag accggatttc
      181 gacggatgat ggcggaagct gtcatctctg gcttgggcgg ggcgtaccgc ctgccgttaa
      241 ccgtcgtcgc tgaagggggg atgtatggcc ataaaaggtc ttgagcaggc cgttgaaaac
      301 ctcagtcgta tcagcaaaac ggcggtgcct ggtgcgtcag caatggccat taaccgcgtt
      361 gcttcatccg cgatatcgca gtcggcgtca caggttgccc gtgagacaag ggtacgccgg
      421 aaactggtaa aggaaagggc caggctgaaa agggccacgg ttaaaaatcc gcaggcagaa
      481 tcaaggttaa ccggggggat ttgcccgtaa tcaagctggg taacgcgcgg gttgtcctgt
      541 cccgacgcag gcgtcgtaaa aaggggcagc gttcatccct gaaaggtggc ggcagcgtgc
      601 ttgtggtggg aaaccgtcgt attcccggcg cgtttattca gcaactgaaa aatggccggt
      661 ggcatgtcat gcagcgtgtg gccgggaaaa accgttaccc cattgatgtg gtgaaaatcc
      721 cgatggcggt gccgctgacc acggcgttta aacagaatat tgagcggata cggcgtgaac
      781 gtcttccgaa agagctgggc tatgcgctgc agcatcaact gagaatggta ataaagcgat
      841 gaaacatact gaactccgtg cagccgtact ggatgcactg gagaagcatg acaccggggc
      901 gacgtttttt gatggtcgcc ccgctgtttt ggatgaggcg gattttccgg cagttgccgt
      961 ttatctcacc ggcgctgaat acacgggcga agagctggac agcgatacct ggcaggcgga
     1021 gctgcatatc gaagttttcc tgcctgctca ggtgccggat tcagagctgg atgcgtggat
     1081 ggagtcccgg atttatccgg tgatgagcga tatcccggca ctatcagatt tgatcaccag
     1141 tatggtggcc agtggctatg actaccggcg cgacgatgat gcgggcctgt ggagttcagc
     1201 cgatctgact tatgtcatta cctatgaaat gtgaggacga tatgccaaca ccaaatcctc
     1261 tggcaccggt gaaaggggcc gggaccacac tgtgggttta taacgggaac ggcgacccat
     1321 atgcaaaccc gctttcagac aatgactggt cgcgtctggc aaaggttaaa gacctgacgc
     1381 ccggcgaact gaccgctgag tcctatgacg acagttatct cgatgatgaa gatgcggact
     1441 gggccgcgac cggacagggg cagaaatccg ctggagatac cagcttcacg ctggcgtgga
     1501 tgcccggaga gcaggggcag caggcgctgc tggcgtggtt taatgaaggt gatacccgtg
     1561 cctataaaat ccgcttcccg aacggcacgg tcgatgtgtt ccgcggctgg gtcagcagta
     1621 tcggtaaggc ggtgacggcg aaggaagtga tcacccgcac ggtgaaagtc accaacgtgg
     1681 gacgtccgtc gatggcagaa gatcgcagca cggtaacagc ggcaaccggc atgaccgtga
     1741 cgcctgccag cacctcggtg gtgaaagggc agagcacgac gctgaccgtg gcattccagc
     1801 cggaaggcgc aaccgacaag agcttccgtg cggtgtctgc ggataaaaca aaagccaccg
     1861 tgtcggtcag tggtatgacc atcaccgtga aaggtgttgc tgcaggcaag gtcaacattc
     1921 cggtcgtatc cggtaatggt gagtttgctg cggttgcaga aatcaacgtc accgccagtt
     1981 aatccggaga atcagcgatg ttcctgaaaa ccgaatcatt tgaacataac ggcgtgaccg
     2041 tcacgctttc tgaactgtca gccctgcagc gtattgagca tctcgccctg atgaaacggc
     2101 aggcagaaca ggcggagtca gacagcaacc ggaagtttac tgtggaagac gccatcagaa
     2161 ccggcgcttt tgtggtggcg atgtccctgt ggcataacca tccgaagaag acgcagatgc
     2221 cgtctatgaa tgaagccgtt aaacagattg agcaggaagt gcttaccacc tggcccgcgg
     2281 aggcaatttc tcatgctgaa aacgtggtgt accggctgtc cggtatgtat gagtttgtgg
     2341 tgaatgatgc ccctgaacag gcagaggacg ccgggcctgc agagcctgtt tctgcgggaa
     2401 agtgttcgac ggtgagctga gttttgccct gaaactggcg cgcgagatgg ggcgacccga
     2461 ctggcgtgcc atgcttgccg ggatgtcatc cacggagtat gccgactggc accgctttta
     2521 cagtacccat tattttcatg atgttctgct ggatatgcac ttttccgggc tgacgtacac
     2581 cgtgctcagc ctgtttttca gcgatccgga tatgcatccg ctggatttca gtctgctgaa
     2641 ccggcgtgag gctgacgaag agcctgaaga tgatgtgctg atgcagaaag cggcagggct
     2701 tgctggaggc gttcgttttg gcccggacgg gaatgaagtt atccccgctt ccccggatgt
     2761 ggcggacatg acggaggatg acgtaatgct gatgacagta tcagaaggga tcgcaggagg
     2821 agtccggtat ggctgaaccg gtaggcgatc tggtcgttga tttaagtctg gatgcggcca
     2881 gatttgacga gcagatggcc agagtcaggc gtcatttttc cggtacggaa agtgatgcga
     2941 aaaaaacagc ggcagtcgtt gaacagtcga tgagccggca ggcgctggct gcacagaaag
     3001 cgggaatttc cgtcgggcag tataaagccg ccatgcgtat gctgcctgcg cagttcactg
     3061 acgtggccac gcagcttgca ggtgggcaaa gtccgtggct gatcctgctg caacaggggg
     3121 ggcaggtgaa ggactccttc ggcgggatga tccccatgtt ccgggggctt gccggtgcga
     3181 tcaccctgcc gatggtgggg gccacctcgc tggcggtggc gaccggtgcg ctggcgtatg
     3241 cctggtatca gggcaactca accctgtccg atttcaacaa aacgctggtc ctttccggca
     3301 atcaggccgg gctgacggca gatcgtatgc tggtcctgtc cagagccggg caggcggcag
     3361 ggctgacgtt taaccagacc agcgagtcac tcagcgcact ggttaaggcg ggggtaagcg
     3421 gtgaggctca gattgcgtcc atcagccaga gtgtggcgcg tttctcctct gcatcaggcg
     3481 tggaggtgga caaggtcgct gaagccttcg ggaagctgac cactgacccg acgtcggggc
     3541 tgacagcgat ggcgcgccag ttccataacg tgacggcgga gcagattgcg tatgttgctc
     3601 agttgcagcg ttccggcgaa gaagccgggg cattgcaggc ggcgaacgag gccgcgacga
     3661 aagggtttga tgaccagacc cgccgcctga aagagaacat gggcacgctg gaaacctggg
     3721 cagacaggac agcacgggca ttcaaatcca tgtgggatgc ggtgctggat attggtcgtc
     3781 ctgataccgc gcaggagatg ctgattaagg cagaggccgc gtttaagaaa gcggacgata
     3841 tctggaatct gcgcaaggat gattattttg ttaacgatga agcgcgggcg cgttactggg
     3901 atgatcgtga aaaggcccgt cttgcgcttg aagccgcccg aaagaaggct gagcagcaga
     3961 gtcaacagga caaaaatgcg cagcaacaga gcgataccga agcgtcacgg ctgaaatata
     4021 ccgaagaggc gcagaaggct tacgaacgcc tgcagacgcc gctggagaaa tataccgccc
     4081 gtcaggaaga actgaataag gcactgaaag acgggaaaat cctgcaggca gattacaaca
     4141 cgctgatggc ggcggcgaaa aaggactatg aagcgacgct gaaaaagccg aaacagtccg
     4201 gcgtgaaggt gtctgcgggc gatcgtcagg aagacagtgc tcatgctgcc ctgctgacgc
     4261 ttcaggcaga actccggatg ctggagaagc atgccggagc gaatgagaaa atcagccagc
     4321 agcgccggga tttgtggaag gcagaaagtc agttcgcggt actggaggag gcggcacaac
     4381 gtcgccagct gtccgcacag gagaaatccc tgctggcgca taaagacgag acgctggagt
     4441 acaaacgcca gctggctgta cttggcgaca aggtcaccta tcaggagcac ctgaacgcgc
     4501 tggcacagca ggcggataaa ttcgcacagc aacaacgggc aaaacgggcc gccattgatg
     4561 caaaaaaccg ggggctgact gaccggcagg cagcgcggga agccacggaa cagcgcctga
     4621 aggaacagta tggcgataat cctctggcgc tgaataacgt catgtcagag cagaaaaaga
     4681 cctgggcagc tgaagaccag cttcgcggga gctggatggc aggcctcagg tcaggctgga
     4741 gtgagtggga agagagcgcc acggacagta tgtcgcaggt taaaagtgct gccacgcaga
     4801 cctttgatgg tattgcacag aatatggcgg cgatgctgac cggcagtgaa cagaactggc
     4861 gcagcttcac ccgttccgtg ctgtccatga tgacagaaat tctgcttaag caggcaatgg
     4921 tggggattgt cggaagtata ggcagcgcca ttggcggcgg cgcatcagcg tcaggcggta
     4981 cagccattca ggcagctgcg gcgaaattcc attttgcggc cggagggttt acgggaaccg
     5041 gcggcaaata tgagccagcg gggattgttc accgtggtga atttgtcttc acgaaggagg
     5101 caaccagccg gattggcgta ggaaatcttt accggctgat gcgcggctat gccaccggcg
     5161 gttatgtcgg tacaccgggc agcatggcgg acagccggtc gcaggcgtcc gggacgtttg
     5221 agcagaataa ccatgtggtg attaacaacg acggcacgaa cggtcagata gggccacagg
     5281 cactgaaggc ggtttatgac gtagcccgta aggcggcaat ggatgttgtg accgggcaga
     5341 tgcgcgatgg tggtctgttc tccggaggtg gacgatgaaa accttccgct ggaaagtgaa
     5401 acccggtatg gatgtggctt cggccccttc cgtaagaaag gtgcgctttg gtgatggcta
     5461 ttcccagcga gcgcctgccg ggctgaatgc cgacctgaaa acgtacagcg tgacgctttc
     5521 tgttccccgt tgggaggcca cggcgcttga gtcgtttctg gctgagcacg ggggctggaa
     5581 ggcctttctg tggacgccgc cttatgagtg gcggcagata aaggtgacct gcgcaaaatg
     5641 gtcgtcgcgg gtcagtatgc tgcgtgttga gttcagcgca gagtttgaac aggtggtgaa
     5701 ctgatgcagg atatccggca ggaaacactg aatgaatgca cccgtgcgga gcagtcggcc
     5761 agcgtggtgc tctgggaaat cgatctgaca gaggtcggtg gagaacgtta ttttttctgt
     5821 aatgagcaga acgaaaaagg tgagccggtc acctggcagg ggcgacagta tcagccgtat
     5881 cccattcagg ggagtggttt tgaactgaat ggcaaaggca ccagtacgcg ccccacgctg
     5941 acggtttcta acctgtacgg tatggtcacc gggatggcgg aagatctgca gagtctggtc
     6001 ggcggaacgg tggtccggcg taaggtttac gcccgttttc tggatgcggt gaacttcgtc
     6061 aacggaaaca g
//
LOCUS       DAZFAB010000181         6070 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZFAB010000181 DAZFAB010000000
VERSION     DAZFAB010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6070
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.181"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..2390)
                     /locus_tag="ACHJWA_004564"
     CDS             complement(<1..2390)
                     /locus_tag="ACHJWA_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO4311014.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AAAREQRRALAELNSQLTEIRGSAVGMAGAFAGAFASGHLISLADEWSSVNARLKQAS
                     QSSDEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSAGDVLKVTEAISTG
                     LKISGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIVRALAAGMGVARKDLK
                     AMADDGKLTADKVVPALISQLGILRDEYAAMPETVSSSITKVENAFMAWVGGANEASG
                     VTKTLSGMLNGVAGQIDNVATAVGALVAVGVARYFGNMASGAMSATAGLVTAARNEVA
                     LAEAQFRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAATQERLNRNIAART
                     AAQNALNSTTAVGSRLMSGALGLVGGVPGLVMLGAAAWYTLYQNQEQARESARQYALT
                     IDEIAHKTPSMSLPEASDNEGRTRAALTEQNRLIDEQASRVKSLQEKIAGYQYVLANP
                     GWTTGDGFMINHLTSVKTVTEGLAQATEQLAVEQSRLAQMQEKAQSIQDVLAGLEDRR
                     VALIRQQAAEQNKVYQSMLVMNGQYTEFNRLLGLGNELLQQRQGLVNVPLRLPQATLD
                     DKQQSALTKTERELALSRLKGEEKERVRLGYAADDLGFVGDPYQEARQRYISNALEAW
                     RNNEANKPKSRGGKSETEKAEDSFSRLLKQQKEQLALAGQNTELAKLKYQTALGELKT
                     LSEIQKQELLRNAALIDQQKIREQLRYREETLKNDNVAARASNESELLGYGQGERA"
     gene            complement(2443..2652)
                     /locus_tag="ACHJWA_004565"
     CDS             complement(2443..2652)
                     /locus_tag="ACHJWA_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFO4311015.1"
                     /translation="MWMEFDRVSPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEVDPFAALEDALSLAAMS"
     gene            complement(2748..3122)
                     /locus_tag="ACHJWA_004566"
     CDS             complement(2748..3122)
                     /locus_tag="ACHJWA_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFO4311016.1"
                     /translation="MAKDLKTLALARLSGFRHKTVKVPEWRNVSVVLREPSAEAWYLW
                     QEVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
     gene            complement(3128..3844)
                     /locus_tag="ACHJWA_004567"
     CDS             complement(3128..3844)
                     /locus_tag="ACHJWA_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFO4311017.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMEKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDTVYAFKVQ
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSYVVPLAFVKNLEKTLTVN
                     TGALLTMSVSVNGGTPPYKHAWKKDGQPVEGQTTDTFSKANTQSGDKGAYTCEVTDSA
                     EQPQSITSDACTVTVNGAGG"
     gene            complement(3903..4247)
                     /locus_tag="ACHJWA_004568"
     CDS             complement(3903..4247)
                     /locus_tag="ACHJWA_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HFO4311018.1"
                     /translation="MTEADLYPHLAHLAGGQVYPYVVPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVSVQIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENR
                     CYRATLEFQVTV"
     gene            complement(4244..4690)
                     /locus_tag="ACHJWA_004569"
     CDS             complement(4244..4690)
                     /locus_tag="ACHJWA_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFO4311019.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VVSRAPVRRGKLRRNVVVLSRCSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
     gene            complement(4687..5037)
                     /locus_tag="ACHJWA_004570"
     CDS             complement(4687..5037)
                     /locus_tag="ACHJWA_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFO4311020.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDHVLNVVGVPVPDAT
                     GGRLEILCRLGGEK"
     gene            complement(5047..5373)
                     /locus_tag="ACHJWA_004571"
     CDS             complement(5047..5373)
                     /locus_tag="ACHJWA_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO4311021.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDEMLMDKVRQATAVLLAYIQGSR
                     DKVISEDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREDLVQGELPFSVSVLIYDL
                     RCPTVL"
     gene            complement(5370..6070)
                     /locus_tag="ACHJWA_004572"
                     /pseudo
     CDS             complement(5370..6070)
                     /locus_tag="ACHJWA_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365916.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
BASE COUNT         1242 a         2015 c         1435 g         1378 t
ORIGIN      
        1 ctggctcgtt ccccctgccc gtaccccagc agttcagatt catttgatgc acgcgcagcc
       61 acattatcat tcttcagggt ctcttcccgg taccgcaact gctcccggat tttttgctgg
      121 tcaatcaggg ccgcattgcg cagcagttcc tgcttctgta tctccgacag ggttttcagt
      181 tcacccagcg ctgtctggta cttcagcttc gccagctccg tgttctgacc ggccagtgcc
      241 agttgctctt tctgctgctt cagcagccgg gaaaaactgt cttccgcttt ttccgtctct
      301 gattttccac cccgggattt gggtttattc gcctcgttat tgcgccaggc ttccagggca
      361 ttactgatat aacgttgtct cgcctcctga tacggatccc ccacaaaacc gaggtcatcc
      421 gccgcatacc ccagtcggac gcgctctttt tcttcccctt tcagtctgga cagggccagc
      481 tcacgctctg tttttgtcag ggcactctgc tgtttatcat ccagagtggc ctgtggcagc
      541 cgtaacggca cattcaccag tccctgccgc tgctgaagca gttcattccc cagccccagc
      601 agacggttga attccgtata ctgaccgttc ataaccagca tggactggta caccttattc
      661 tgctctgccg cctgctgacg aattaacgcc acacgacggt cttccagccc ggcaagcaca
      721 tcctgaatgg actgcgcttt ttcctgcatc tgtgccagac gggactgctc aacggcaagc
      781 tgctctgttg cctgagcaag cccttccgtt acggtcttca ccgatgtcag atggtttatc
      841 atgaatccgt caccggtcgt ccagcccggg ttcgccagaa catactgata tcctgcgatt
      901 ttttcctgca gggatttcac ccgactggcc tgttcatcaa tcagccggtt ctgctctgtc
      961 agcgccgccc gtgttcgtcc ttcattatct gaggcttcag gcagagacat tgacggcgtt
     1021 ttatgcgcga tttcatctat cgtcagtgca tactggcgcg cagactccct ggcctgctcc
     1081 tgattctggt acagcgtgta ccatgctgca gcccccagca tcaccagtcc gggtacgcca
     1141 ccaaccagcc ccagcgcacc gctcatcaga cgtgagccca ccgccgttgt actgttcagc
     1201 gcattctggg cggcggttct ggcagcaata tttctgttca ggcgttcctg tgtggccgcc
     1261 agacgggcct ctgctgcaat ctgcatctcc gtcccgcggg ctgccgccac ggcctgctga
     1321 gcacggtaca cggctgccct tgcccgcgcc gtggcaatct gcgttcccct gaactgtgct
     1381 tccgccagtg caacttcatt acgtgcagcc gtcacaagtc ctgccgtggc agacatcgct
     1441 ccggaggcca tattgccaaa gtaacgtgca accccgacgg caaccagcgc gcccacggct
     1501 gttgccacat tatcaatctg tccggcaaca ccgttcagca tgccggagag cgtttttgtc
     1561 accccgctgg cctcattcgc accgcccacc caggccataa aggcgttttc caccttcgtg
     1621 atactactgg aaaccgtttc cggcatggcc gcatattcat cacgtaatat ccccagctgg
     1681 ctgattaacg cggggaccac tttatccgct gtcagttttc cgtcatccgc cattgccttc
     1741 agatctttac gggccacgcc catacccgca gccagtgcac gtacgatccg gtcaccactt
     1801 tcattgaccg aattaaattc ctcaccgcgc aacacaccct gtgccagcgc ctggctgaac
     1861 tgggtgatca ccgaacccgc ctcagccgta ctggcaccgg agattttcag ccccgtggaa
     1921 atggcctccg tcaccttcag tacatcaccg gcactgtaac catattcacg catcgaggca
     1981 gccgaacggg caaacagggc cgcattatcc gaaaatgccg tgcctgtccg ctggctgata
     2041 tccatcagca ctttctgtga tgacgaaaat tcatcggatg actgtgatgc ctgtttcaga
     2101 cgggcattta cggaactcca ctcatccgcc agtgaaatca ggtgtccgga ggcaaaggca
     2161 ccggcaaatg cgccagccat tccgacagca gaaccgcgaa tttccgtcaa ctggctgttc
     2221 agttctgcca gggcacgtcg ctgctcccgg gctgccgcag cggcctgacg cccgccattc
     2281 tgcagggtcc ggtaatattc actgcccata cgggacgccc gctggatctc cgactggaat
     2341 gactgtgaat ttgccgaaat tttgataatc agttcacgta acgtcgccat tcacctttct
     2401 ccgggcaaaa aaaaacctgc cacagcaggt tttcatcatt atttatgaca ttgctgcaag
     2461 gctcagcgcg tcttccagcg ccgcaaacgg atccacctcc ggcttatcct catcctcgcc
     2521 ccagcagagc atggcgtcct tcagtgcaac attcatcccc tgtgccccga aaaccgcttt
     2581 cacgatctgt gcattacgga tatccccgcg ctcatcaccc agcggggata ccctgtcgaa
     2641 ctccatccac atcatcgcct cgctcgcact caggctgtgc cgcagttcgg ataaggtgcg
     2701 ccccagacgg agcgcaagtc gcatcagaaa gcgaatttcc gggcgggcta cttttttctg
     2761 gccgactctg catcagcgat cagttccagt gcctgacgca gcaaccgggc atgtaccgga
     2821 ccatagacgg ccagcacctg ctcacggtcg tccggagtga acacccgttg caggtcagta
     2881 tcacacagga catcgcagaa cagcgtcaca tccgcttcca ggttacggcg ggttttcgcc
     2941 accaccgaca gggtatcgtc atcctctcca tcaccattga gcacttcctg ccacagatac
     3001 caggcctctg ccgaaggctc ccgcagcacc acgctgacat ttctccattc cggcaccttc
     3061 accgttttat gacggaaccc cgacagtctg gccagcgcca gtgttttcag atcttttgcc
     3121 ataagcctta tccgcccgca ccattaaccg ttactgtaca cgcatcagag gtaatgctct
     3181 gcggctgttc tgcagaatcc gttacctcgc aggtataagc ccccttatca cctgactgcg
     3241 tattggcttt actgaaagtg tcagtagtct gtccctctac cggctgacca tccttcttcc
     3301 aggcgtgttt ataaggcggc gttcccccgt tgacactgac tgacattgtc agcagcgcac
     3361 cggtattcac ggtaagtgtc ttctccagat ttttcacaaa cgccagcggt accacatagg
     3421 acaccggttt acccttcagg cgaagtgaaa acgttgcagc caccacgccg ttggtaccgg
     3481 atgaccaggt gtgctgacgc acttccgcca ggaacttaaa gcccttaccg gacggaaact
     3541 gcaccttaaa cgcatacacc gtgtcattgt cataggcatc acgcagggcg ttctgggcct
     3601 gattcagata aaaattaccc gacatggaaa tctcggacga cgcccccaga ccgttgatgt
     3661 tctcctgctc tgtggagcag agcgtggtca catcaatatc ctgtttctga ccggcggtga
     3721 actggacttc cttgatggtg cagtccaggc gcagatattc cgccttctcc atggtttcag
     3781 cagtcgccgg ggcagatgaa atcatcacct gcgtcagctg tgaacgttca tacaaagcag
     3841 acattctgcc tcctgataat aaaaaacccg cacgcggcgg gttatgggtt ctgttgaaaa
     3901 aattacaccg tgacctgaaa ctccagggtt gcacggtaac agcggttttc cggaatatag
     3961 tcctgcattt cactgacgga tcccggggcc agcagcatta tggcttcacg ggcgtcctga
     4021 cgtatctgac gcgcctgcgt cacagtcccg gcataaacgt ctatctgcac cgacactgag
     4081 gactccgcct gcccgcccat cacgtccgcc gacaccgatg aaatcaggct gaaaaccacc
     4141 cacggaagcg ccaccgacgg cctgccatcc agcaggggga ccacatacgg gtacacctgc
     4201 ccgccggcaa gatgcgccag atgaggatac aaatccgcct ccgtcatcgt ctcagtacct
     4261 catcaatggc ccggttcatc cgagcaatcg ccacctgtgc cgcctgttca ctgcgcacat
     4321 caaatgccgg gcgcacaaac gggtgcggtg gcatattcac ggtccccatt tccacaaacc
     4381 gccagtagaa agcattgcgc gggttatccg ccttcatggt gttatcgctg ttaccggtgt
     4441 ccggattaac accccggata tgcacaccgg attccatccc gccatcgcgg gagcaccggg
     4501 aaaggaccac cacattgcgg cgaagttttc ccctgcgtac cggtgcccgt gacaccactt
     4561 cttctttcag cacattcgca cccgcacggg ttgcctcacg cagcacccgg ttattttccg
     4621 caccactcag aagctgcaaa tcgcggctga tgtcctccag ccccgaaaaa tccagcaggg
     4681 tttcgatcat ttttcccctc ccagccgaca gagaatttcc agacgcccgc cggttgcatc
     4741 cggaaccggg accccgacga cattcaggac atggtcacgc cagggaccac tcagcacatg
     4801 aagtcgtgac gccgccgtga tttcacgacc agactgaccg cgtacccaga tgcggatttc
     4861 cgcctgcgcc atttccgcac cggactgcat ccgctcccgg ctgctcctgc cccggatatc
     4921 cgcatgaatt ttcccgcatg acacccattc ttccgtcatt tctccggcag cattacgggt
     4981 taacaccggg ttcagaacac tgatcatctg tgtcagacga cctgcagata ttgccattcc
     5041 ccctcctcat aacaccgtcg gacaacgcaa atcgtaaatc agcacggaaa cagaaaacgg
     5101 cagttccccc tgcacgaggt cttcccgctc agcaagatcc ggattccggt acagcatccc
     5161 ggtcagtcgc atggcagccc ccttcatccg ggttaatgcc tcaccaggga tcagctcacc
     5221 gtcctcacta atcactttat cccggctgcc ctggatgtag gccagcagca cggcggtagc
     5281 ctgacgaacc ttgtccatca gcatctcatc atccgcgtca tggtcaacac gcagatgtgc
     5341 cttgatttct tccagtgtca gtaatgctgt cactttccac ctcctgcatc ccgcccacgt
     5401 tttgcagcca gagtccagcc tgatgaatga gcttctccgg gcttatcacc ggtcatactg
     5461 ttgcagtgcc acaacgagcc ccccacgtca ccgtatcgcc ggggtggtaa gtttcgccga
     5521 ttctgaacac accacggtag agcatcaccg gcagggaaaa tgttttttcc gtacgctggc
     5581 cactgctctg ccggaccacc acagagaacg accgttcacc cgtcatgctg acgtcaatat
     5641 ccgccacccc gtcaaccagg cattcccatc cccgcatccc gtacgttttt tcatacgccc
     5701 gccagagtcc accctggtgt gtggcatacg agccccgggg aaaggatttt tgatcgtcaa
     5761 tggcggggag tatttccagt gccgtggcat cacgcccgtc ctgcggagcc ggcagggcac
     5821 tcaccgcctc cagaaccgcc ttctgcagaa catccggatc gtagtcacga ccatcacgcg
     5881 gaacaggaat atggcttacc gcctccttca ccatctgttc aagcatcgga tgcacatcat
     5941 ccggagtgat acttttaccg tctgccggta ccggtatttt cgcgaccgca tcattcaccg
     6001 ccttctgcag aacatccgga tcgtagtcac gaccgtcgcg cggaacagga atatggctca
     6061 ctgcctcttt
//
LOCUS       DAZFAB010000182         6049 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZFAB010000182 DAZFAB010000000
VERSION     DAZFAB010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6049
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.182"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..2390)
                     /locus_tag="ACHJWA_004573"
     CDS             complement(<1..2390)
                     /locus_tag="ACHJWA_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO4311022.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AAAREQRRALAELHSQLTEIRASAVGMTGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFASSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTG
                     LKISGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIVRALAAGMGVARKDLK
                     AMADDGKLTADKVVPALISQLGILRDEYAAMPETVSSSITKVENAFMAWVGGANEASG
                     VTKTLSGMLNGVAGQIDNVATAVGALVAVGVARYFGNMASGAMSATAGLVTAARNEVA
                     LAEAQFRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAATQERLNRNIAARS
                     AAQNALNSTTAVGSRLMSGALGLVGGVPGLVMLGAAAWYTLYQNQEQARESARQYALT
                     IDEIAHKTPSMSLPEASDNEGRTRAALTEQNRLIDEQASRVKSLQEKIAGYQYVLANP
                     GWTTGDGFMINHLTSVKTVTEGLSQATEQLAVEQSRLAQMQEKAQSIQDVLAGLEDRR
                     VALIRQQAAEQNKVYQSMLVMNGQHTEFNRLLGLGNELLQQRQGLVNVPLRLPQATLD
                     DKQQSALTKTERELALSRLKGEEKERVRLGYAADDLGFVGDPYQEARQRYISNALEAW
                     RNNEANKPKSRGGKSETEKAEDSFSRLLKQQKEQLALVGQNTELAKLKYQTALGELKT
                     LTEMQKQELLRNATLIDQQKIREQLRSREETLKNENAAARASNDAELLGYGQGERA"
     gene            complement(2438..2647)
                     /locus_tag="ACHJWA_004574"
     CDS             complement(2438..2647)
                     /locus_tag="ACHJWA_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFO4311023.1"
                     /translation="MWMEFDRISPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEVDPFAALEDALSLAAQS"
     gene            complement(2743..3117)
                     /locus_tag="ACHJWA_004575"
     CDS             complement(2743..3117)
                     /locus_tag="ACHJWA_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFO4311024.1"
                     /translation="MKKDLKTLALARLSGFRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     QEVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
     gene            complement(3132..3848)
                     /locus_tag="ACHJWA_004576"
     CDS             complement(3132..3848)
                     /locus_tag="ACHJWA_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFO4311025.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMDKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDALYAFKVL
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSFVVPLAFVKNLDKTLTVN
                     TGALLTMSVSANGGTPPYKYAWKKDGQPVDGQTTDTFSKPGAQSADAGKYTCVVTDSA
                     EKAQSVTSVECTVTVSAAAG"
     gene            complement(3914..4258)
                     /locus_tag="ACHJWA_004577"
     CDS             complement(3914..4258)
                     /locus_tag="ACHJWA_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HFO4311026.1"
                     /translation="MTEADLYPHLAHLAGGQVYPYVVPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVSVQIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENR
                     CYRATLEFQVTV"
     gene            complement(4255..4701)
                     /locus_tag="ACHJWA_004578"
     CDS             complement(4255..4701)
                     /locus_tag="ACHJWA_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFO4311027.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VVSRAPVRRGKLRRNVVVLSRRSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
     gene            complement(4698..5048)
                     /locus_tag="ACHJWA_004579"
     CDS             complement(4698..5048)
                     /locus_tag="ACHJWA_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFO4311028.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDAT
                     GGRLEILCRLGGEK"
     gene            complement(5058..5384)
                     /locus_tag="ACHJWA_004580"
     CDS             complement(5058..5384)
                     /locus_tag="ACHJWA_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO4311029.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREELIQGELPFSVSVLIYDL
                     RCPTVL"
     gene            complement(5387..>6049)
                     /locus_tag="ACHJWA_004581"
     CDS             complement(5387..>6049)
                     /locus_tag="ACHJWA_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4311030.1"
                     /translation="DGRDYDPEVLQKAVNDAVANIPQPADGKSLTPDDVRPMLEQMVK
                     EAVSHIPVPRDGRDYDPDVLQKAVLDAVSALPAPQDGRDATALEILPAIDDQKSFPRG
                     TYATHQGGLWRAYEKTHGMRGWECLVDGVADIDVSMTGERLFSVVVRQSSGQRTEKTF
                     SLPVMLYRGVFRAGETYHPGDTVTWGGSLWHCNSMTEDKPGEAHSSAWTLAAKRGRDA
                     GG"
BASE COUNT         1217 a         2027 c         1447 g         1358 t
ORIGIN      
        1 ctggctcgct ccccctgccc gtaccccagc agttcagcat cattcgacgc acgcgcagcc
       61 gcattctcat tcttcagtgt ctcttcccgg gatcgcaact gttcccggat tttttgctgg
      121 tcaatcaggg tcgcgttacg cagcagttcc tgcttctgca tctccgtcag ggttttcagt
      181 tcacccagcg ctgtctggta tttcagcttc gccagctctg tattctgccc caccagtgcc
      241 agttgctctt tctgctgctt cagcagccgg gaaaaactgt cttccgcttt ttccgtctct
      301 gattttccac cccgggattt gggtttattc gcctcgttat tgcgccaggc ttccagggca
      361 ttactgatat aacgttgtct cgcctcctga tacggatcac ccacaaaacc gaggtcatcc
      421 gccgcatacc ccagtcggac acgctctttt tcttcccctt tcagtctgga cagggccagc
      481 tcacgctctg tttttgtcag ggcactctgc tgtttatcat ccagggtggc ctgcggcagc
      541 cgtaacggta cattcaccag tccctgacgc tgctgaagca gttcattccc cagccccagc
      601 agacggttga attccgtatg ctgaccgttc ataaccagca tggactggta caccttattc
      661 tgctctgccg cctgctgacg aattaacgcc acacgacggt cttccagccc ggcaagcaca
      721 tcctgaatgg actgcgcttt ttcctgcatc tgtgccagac gggactgctc aacggcaagc
      781 tgctctgttg cctgagaaag cccttccgtt acggtcttca ccgatgtcag atggtttatc
      841 atgaatccgt caccggtcgt ccagcccggg ttcgccagaa catactgata tcctgcgatt
      901 ttttcctgca gggatttcac ccgactggcc tgttcatcaa tcagccggtt ctgctctgtc
      961 agcgccgccc gtgttcgtcc ttcattatct gaggcttcag gcaaagacat tgacggcgtt
     1021 ttatgcgcga tttcatctat cgtcagtgca tactggcgcg cagactccct ggcctgctcc
     1081 tgattctggt acagcgtgta ccatgctgca gcccccagca tcaccagtcc gggtacgcca
     1141 ccaaccagcc ccagcgcacc gctcatcaga cgtgagccca ccgccgttgt actgttcagc
     1201 gcattctggg cggcgcttct ggcagcaata tttctgttca ggcgttcctg tgtggccgcc
     1261 agacgggcct ctgcagcaat ctgcatctcc gtcccgcggg ctgccgccac ggcctgctga
     1321 gcacggtaca cggctgccct tgcccgcgcc gtggcaatct gcgttcccct gaactgtgct
     1381 tccgccagtg caacttcatt acgtgcagcc gtcacaagtc ctgccgtggc agacatcgct
     1441 ccggaggcca tattgccaaa gtaccgggca accccgacgg caaccagcgc gcccacggct
     1501 gttgccacat tatcaatctg tccggcaaca ccgttcagca tgccggagag cgtttttgtc
     1561 accccgctgg cctcattcgc accgcccacc caggccataa aggcgttttc cacctttgtg
     1621 atactactgg aaaccgtttc cggcatggcc gcatattcat cacgtaatat ccccagctgg
     1681 ctgattaacg cggggaccac tttatccgct gtcagttttc cgtcatccgc cattgccttc
     1741 agatctttac gggccacgcc catacccgca gccagtgcac gtacgatccg gtcaccactt
     1801 tcattgaccg aattaaactc ctcaccacgc aatacaccct gcgccagcgc ctgactgaac
     1861 tgggtgatca ccgagcccgc ctctgccgta ctggcaccgg agattttcag ccctgtcgaa
     1921 atggcctccg tcaccttcag cacatcatca gcactgtaac catattcacg catcgaggca
     1981 gccgaacggg caaacagggc cgcattatcc gaaaatgcgg tgcctgtccg ctggctgata
     2041 tccatcagca ctttctgtga tgacgcaaat tcatccgatg actgcgacgc ctgtttcaga
     2101 cgggcattca cggagctcca ttcatccgcc agcgaaatca ggtgtccggt ggcaaaggca
     2161 ccggcaaacg caccggtcat tccgacagcc gaagcgcgga tttccgtcaa ctggctgtgc
     2221 agctcagcca gagcccggcg ctgctccctg gctgccgcag cagcctgacg tccgccattc
     2281 tgcagggtcc ggtaatattc actgcccata cgggacgccc gctggatctc cgactggaat
     2341 gactgtgaat ttgccgaaat tttgataatc agttcacgta acgtcgccat tcacctttct
     2401 ccgggcgtaa aaaaaccgcc tcagcggttc tcatcattca tgactgtgct gcaaggctca
     2461 gcgcgtcttc cagcgccgca aacggatcca cctccggctt atcctcatcc tcgccccagc
     2521 agagcatggc gtccttcagt gcaacattca tcccctgtgc cccgaaaacc gctttcacga
     2581 tctgtgcatt acggatatcc ccgcgctcat cacccagcgg ggatatcctg tcgaactcca
     2641 tccacatcat cgcctcgctc gcactcaggc tgtggcgcag ttcggataag gtgcgcccca
     2701 gacggagcgc aagtcgcatc agaaagcgaa tttccgggcg ggctactttt ttctggccga
     2761 ctctgcatca gcgatcagtt ccagtgcctg acgcagcaac cgggcatgta ccggaccata
     2821 gacggccagc acctgctcac ggtcgtccgg agtgaacacc cgctgcaggt ccgtatcaca
     2881 caggacatcg cagaacagcg tcacatccgc ttccaggtta cggcgggttt tcgccaccac
     2941 cgaaagggta tcgtcatcct ctccatcacc attgagcact tcctgccaca gataccaggc
     3001 ctctgccgaa ggctcccgca gcaccacgct gacattaccc cattccggca ccttcaccgt
     3061 tttatgacga aaccctgaca gtctggccag cgccagcgtt ttcagatcct ttttcatgat
     3121 gacccatccc cttatccggc ggctgcgctc actgtcacgg tgcattcaac agacgtcaca
     3181 ctctgtgctt tctctgccga atcggtcacc acacaggtat atttccccgc atcagcggac
     3241 tgcgcacctg gcttactgaa ggtgtctgtc gtctgcccgt caaccggctg accatccttc
     3301 ttccaggcgt atttatacgg cggcgttccc ccgttggcac tgactgacat tgtcagcagc
     3361 gcaccggtat tcacggtaag tgtcttatcc agatttttca caaacgccag cggtaccaca
     3421 aaggacaccg gtttgccttt cagacgcagt gagaacgttg cagccaccac gccgttggta
     3481 ccggatgacc aggtgtgctg gcgcacttcc gccaggaact taaagccctt accggacgga
     3541 aacagcacct taaacgcata caacgcgtca ttgtcatagg catcacgcag ggcgttctgg
     3601 gcctgattca gataaaaatt gcccgacatg gaaatctcag acgacgcccc cagaccgttg
     3661 atgttctcct gctctgtgga gcagagcgtg gtcacatcaa tatcctgttt ctgcccggcg
     3721 gtgaactgca cttccttgat ggtgcagtcc aggcgcagat attccgcctt atccatagtt
     3781 tcagcagtcg ccggggcaga tgaaatcatc acctgcgtca gctgtgagcg ttcatacaaa
     3841 gcagacattc tgcctcctga taataaaaaa cccgcacgcg gcggggtatg ggttttgtag
     3901 aaaaaagaaa aagtcacacc gtgacctgaa actccagggt tgcacggtaa cagcggtttt
     3961 ccggaatata gtcctgcatt tcactgacgg atcccggggc cagcagcatt atggcttcac
     4021 gggcgtcctg acgtatctga cgcgcctgcg tcacagtccc ggcataaacg tctatctgca
     4081 ccgacactga ggactccgcc tgcccgccca tcacgtccgc tgacaccgat gaaatcaggc
     4141 tgaaaaccac ccacggaagc gccaccgacg gcctgccatc cagcaggggg accacatacg
     4201 ggtacacctg cccgccggca agatgcgcca gatgaggata caaatccgcc tccgtcatcg
     4261 tctcagtacc tcatcaatgg cccggttcat ccgcgcaatc gccacctgtg ccgcctgttc
     4321 actgcgcaca tcaaatgccg ggcgcacaaa cgggtgcggt ggcatattca cggtccccat
     4381 ttccacaaac cgccagtaga aagcgttgcg cgggttatcc gccttcatgg tgttatcgct
     4441 gttaccggtg tccggattaa caccccggat atgcacaccg gattccatcc cgccatcgcg
     4501 ggagcgccgg gaaaggacca ccacattgcg gcgcagtttt cccctgcgta ccggtgcccg
     4561 tgacaccact tcttctttca gcacattcgc acccgcacgg gttgcctcac gcagcacccg
     4621 gttattttct gcaccactca gaagctgcaa atcgcggctg atgtcctcca gccccgaaaa
     4681 atccagcagg gtttcgatca tttttcccct cccagccgac agagaatttc cagacgtccg
     4741 ccggtcgcat ccggcacggg cagcccgaca acgttcagga tccggtcacg ccagggacca
     4801 ctcagcacat gaagtcgtga cgctgccgtg atttcccgac cggactgacc gcgcacccag
     4861 atgcggattt ccgcctgcgc catttccgca ccggactgca tccgctcccg gctgctcctg
     4921 ccacggatat ccgcatgaat tttcccgcat gacacccatt cttccgtcat ttctccggca
     4981 gcattacggg ttaacaccgg gttcagaaca cttatcatct gtgtcagacg acctgcagat
     5041 attgccattc cccctcctca taacaccgtc ggacaacgca aatcgtaaat cagcacggaa
     5101 acagaaaacg gcagctcccc ctgaatcagt tcttcccgct ccgcaagatc cggattccgg
     5161 tacagcatcc cggtcagtcg catggcagcc cccttcatcc gggttaatgc ctcgcccggg
     5221 atcagttcac cgtcctcacg aatcacctta tcccggctgc cctgaatgta ggccagcagc
     5281 acggcggtag cctgacgaac cttgtccatc agcatgtcat catccgcgtc atggtcgaca
     5341 cgcagatgtg ccttgatctc ttccagtgtc agtaatgccg tcattttcag cctcctgcat
     5401 cccgcccacg ttttgcagcc agggtccagg ctgatgaatg agcttctccg ggtttatctt
     5461 cggtcatact gttgcagtgc cacagcgagc ccccccacgt caccgtatcg ccggggtggt
     5521 aggtttcacc ggctctgaac acaccgcggt agagcatcac cggcagggaa aatgtttttt
     5581 ccgtacgctg gccactgctc tgccggacca ccacagagaa caaccgctca cccgtcatgc
     5641 tgacgtcaat atccgccacc ccgtcaacca ggcattccca tccccgcatc ccgtgcgttt
     5701 tttcatacgc ccgccagagt ccgccctggt gtgtggcata cgtgccccgg ggaaaggatt
     5761 tttgatcgtc aatggcgggg agtatttcca gagccgtggc atcacgcccg tcctgcggag
     5821 ccggcagggc actcaccgca tccagaaccg ccttctgcag aacatccgga tcgtagtcac
     5881 gaccatcacg cggaacagga atatggctta ccgcctcctt caccatctgt tcaagcatcg
     5941 gacgcacatc atccggggtg agacttttac cgtctgccgg ctgcggaata tttgcgaccg
     6001 catcattcac cgccttctgc agtacttccg gatcatagtc acgaccgtc
//
LOCUS       DAZFAB010000183         5994 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZFAB010000183 DAZFAB010000000
VERSION     DAZFAB010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5994
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.183"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            165..683
                     /locus_tag="ACHJWA_004582"
     CDS             165..683
                     /locus_tag="ACHJWA_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFO4311031.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            893..3034
                     /locus_tag="ACHJWA_004583"
     CDS             893..3034
                     /locus_tag="ACHJWA_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFO4311032.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFQQILDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQKLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIASILGTILQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVLYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     RGSGTTLDNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAEKIGNNQAITVTNNQILNIGVNQIQTVGVNQVETVGSNQIIKVGSNQV
                     EKVGIIRALTVGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYSVNVGNNVTFS
                     VGKTMKENTGQTAVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHLEGESDVNGDAP
                     VINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
     gene            3110..>5994
                     /locus_tag="ACHJWA_004584"
     CDS             3110..>5994
                     /locus_tag="ACHJWA_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFO4311033.1"
                     /translation="MGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGIT
                     YANPVNPVLGAKVLPGETDLALPGPLPFILSRAYSSYRTRTPAPVGVFGPGWKAPFDI
                     RLQIRDEGLILNDNGGRSIHFEPLFPGEISYSRSESFWLARGGVAEQHSSQPLSALWQ
                     VLPEDVRLSPHMYLATNSLQGPWWILNWPERVPGADEVLPPEPPAYRVLTGVVDGFGR
                     TLAFHRAAEGDVAGAVTGVTDGAGRRFHLVLTTQAQRAEVFRKQRATSLSSPAGPRSA
                     SSSLVFPDTLPAGTEYGADNGIRLEAVWLTHDPAYPDELPAAPLARYTYTASGELRAV
                     YDRSGTQVRGFAYDAEHAGRMVAHHYAGRPESRYRYDDTGRVTELVNPEGLDYRFEYG
                     QDRVTITDSLNRREVLYTEGEGGLKRVVKKEHADGSITRSEYDEAGRLKAQTDAAGRR
                     TEYSLHMASGAVTAVTGPDGRTVRYGYNSQRQVTSVTYPDGLRSSREYDEKGRLAAET
                     SRSGETTRYSYDDPASELPTGIQDATGSTKQMAWSRYGQLLTFTDCSGYTTRYEYDRY
                     GQQIAVHREEGISTYSSYNPRGQLVSQKDAQGRETRYEYSAAGDLTAIVAPDGSRSEI
                     QYDAWGKAVSTTQGGLTRSMGYDAAGRITVLTNENGSQSTFRYDPVDRLTEQRGFDGR
                     TQRYHYDLTGKLTQSEDEGLITLWHYDASDRITHRTVNGDPAEQWQYDEHGWLTTLSH
                     TSEGHRVSVHYGYDDKGRLTGERQTVENPETGEMLWEHETGHAYSEQGLATRQEPDGL
                     PPVEWLTYGSGYLAGMKLGGTPLVEYMRDRLHRETARSFGGEAYELATAWNTSGQLRS
                     RHLNLPQLDRDYDWNDNGQLIRISGPQESREYRYSDTGRLTGVHTTAANLDIDIPYAT
                     DPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYIYRYDEYGRLAEKTDR"
BASE COUNT         1370 a         1597 c         1930 g         1097 t
ORIGIN      
        1 attttaaaaa tagtttttac gacaaccgta aattctagct cggtttgtat gtaaaaaata
       61 ctgaaaaatg atgcggttcc gtgaagtgag ctgcatctgc ctgtctggga ttaacggcag
      121 gtcattgtaa gtagtaatta accacgttaa caaggagttt acaaatgcca acaccgtgtt
      181 atatctccat cactggtcag acccagggaa atattactgc aggtgcattt accgctgatt
      241 ctgtcggcaa catctatgtg caggggcacg aagacgagat gctggtgcag gaattcctgc
      301 ataacgttac tgtcccgacc gatcctcagt ccggtcagcc tgcgggtcag cgtgctcaca
      361 agcctttcat ttttaccgtg gcgctgaaca aagcggttcc gctgatgtac aacgcactgg
      421 cctccggcga aatgctgcca accactgaac ttcactggtg gcgtacctct gtggaaggca
      481 aacaggagca ttatttcact acccgcctga ctgattcgac gattgtcgat atgaaactgc
      541 atatgccgca ttgccaggac ccggcgaaac gcgagttcac ccagttgctc gaagtttcac
      601 tggcctaccg caaaattgaa tgggaacacg tcaaatctgg cacctccggt gccgacgact
      661 ggcgcgcacc gctggaagca taagttaagc caacagcatc cggctatcag gccggatgct
      721 gtttttgtat tacgccgtag aaagaaaggt tgtcagtaag gggttacgtt cacgggagat
      781 ggcggctgcc ggaaacttcg gcggtagcgt gcctgaacgc aagcgtagca ctgtactgtc
      841 acagccgctt taacagcgaa ctgtctgttt ttccttaacc ccggaggttg ctatgtcaac
      901 cggattacgt ttcacgctgg aagtggacgg cctgccaccg gatgcttttg cggtggtctc
      961 ctttcatctg aaccagtcac tctcttcgct tttttccctc gatctctctc tggtcagcca
     1021 gcagtttctc tcccttgaat tccagcagat cctcgacaaa atggcctacc tgacgatatg
     1081 gcagggcgat gacgtacagc gccgggtgaa aggtgtggtg acctggtttg aactggggga
     1141 gaacgacaaa aaccagaagc tgtacagcat gaaggtatgc ccgccgctgt ggcgcacagg
     1201 gctgcgccag aacttccgta tcttccagaa tgaggacatc gcaagcatcc tcggcacgat
     1261 attgcaggaa aacggggtga ccgagtggag cccgctgttc agcgagccac atccttcccg
     1321 tgagttttgt gtccagtacg gtgagactga ttacgatttc ctgtgccgga tggcggcgga
     1381 ggaaggcatc ttcttttatg aggagcacgc gcaaaaaagt accgaccaga gcctggtcct
     1441 gtgcgatacc gtgctttatc tgccggagtc ctttgagatc ccctggaatc cgaacacccg
     1501 taccgaggta agcaccctct gcatcagcca gtttcgctac agcgcacaaa tccgcccttc
     1561 ttccgtggtg accaaagact acacctttaa acgccccggc tgggccggac gttttgacca
     1621 ggaaggccag caccaggatt accagcgcac acagtatgaa gtgtatgact atcccggacg
     1681 tttcaagggc gcccacgggc agaactttgc ccgctggcag atggacggct ggcgaaacaa
     1741 cgcagaagtg gcgcgcggaa caagccgttc gccggagata tggccgggac ggcgaattgt
     1801 gctgacgggg catccgcagg cgaacctgaa ccgggaatgg caggtggtgg caagtgaact
     1861 gcacggtgag cagccgcagg cggtgccggg acgcaggggt tcaggcacca cgctggataa
     1921 ccattttgcg gtaataccgg cagacagaac atggcgacca cagccgttgc tgaaaccgct
     1981 ggtggacggc ccgcagagcg ccgtcgtgac gggaccggca ggcgaggaaa tcttctgtga
     2041 tgaacatggc cgcgtgcggg tgaaatttaa ctgggaccgc tataacccgt caaaccagga
     2101 cagttcatgc tggatccgtg tggcacaggc gtgggcaggc accgggtttg gtaacctggc
     2161 gataccgcgt gtgggtcagg aggtcattgt ggacttcctc aacggcgatc cggaccagcc
     2221 gatcattatg gggcgtacct accaccagga aaaccgcaca cccggtagcc tgccggggac
     2281 gaagacgcag atgaccattc gttcgaaaac ctataagggc agcgggttta atgaactgaa
     2341 gtttgacgat gcgacaggga aagaacaggt ctacatccac gcgcagaaga acatgaacac
     2401 cgaggtgctg aataaccgca ccacggatgt gataaacaac catgccgaaa aaataggtaa
     2461 caaccaggcg atcaccgtta ccaataacca gatcctgaac attggcgtta atcagataca
     2521 gacggttggt gtcaaccagg tggaaacggt aggcagtaac cagattatca aagtgggatc
     2581 aaaccaggtt gaaaaggtgg ggatcattcg tgcgctgacg gtgggtgtgg cgtatcagac
     2641 gacggtaggc ggcattatga atacctcggt ggcgttgttg cagtcctcac aggtagggct
     2701 gcataaatca ctgatggtgg gaatgggcta cagcgtcaat gtagggaata acgtcacctt
     2761 ctcggtgggc aagacgatga aggaaaacac cggacaaaca gcagtttatt ctgccggtga
     2821 gcatcttgaa ctctgctgtg gtaaggcaag gctggtgctg acgaaggacg gaagcatatt
     2881 tcttaatggt acgcacattc atctggaagg ggagtcggat gtgaacggtg atgcgccggt
     2941 gattaactgg aactgtggtg ccacacaacc tgtaccggat gcgcctgtgc cgaaagattt
     3001 accccccgga atgccggata tgcggcaatt ttgagtcagg tgcaaaaaat caaaacggaa
     3061 ctggtgcagt gaccagataa accatattaa aagcagaagg aattgattca tgggaggaaa
     3121 accggcggcg cggcagggtg acatgacccg caagggactg gatattgtgc agggttcagc
     3181 aggggtgctg ataggtgcgc cgacgggcgt ggcctgctcg gtgtgtccgg gagggattac
     3241 ctatgctaac ccggtgaacc cggtgctggg tgcgaaggtg ctgccgggcg agacggacct
     3301 tgcgctgccc ggcccgctgc cgtttattct ttcccgcgcc tacagcagct accggaccag
     3361 aacacccgcg ccggtggggg tgtttggtcc cggctggaaa gcgcccttcg atatccgctt
     3421 acagatacgc gatgaaggcc tgatactcaa cgacaacggc ggcaggagca ttcactttga
     3481 gccgctgttt cccggcgaga taagctacag ccgcagcgag tcgttctggc tggcgcgggg
     3541 cggggtggcg gagcagcaca gttcgcagcc gctaagcgcg ctctggcagg tgctgccgga
     3601 agatgttcgc ctgagtccgc atatgtacct ggcgacaaac agccttcagg ggccgtggtg
     3661 gatactcaac tggccggagc gggtgccggg ggcggacgag gtgctgccgc cggagccgcc
     3721 cgcataccgg gtgctgacgg gcgtggtgga tggcttcggg cggacgctgg cctttcaccg
     3781 ggcggcggag ggtgatgtgg cgggcgcggt gacgggggtg acggacggcg cgggccgccg
     3841 ttttcacctg gtgctgacca cacaggcaca gcgggcggaa gtattccgta aacagcgcgc
     3901 cacgtcttta tcttcccctg ccggtccccg ttctgcttcc tcttctctgg ttttccccga
     3961 cacgctgccc gccggtacag aatacggtgc cgataacggt atccggctgg aggcggtatg
     4021 gctgacacac gacccggcat acccggatga actgcccgcc gcgccgctgg cgcgctacac
     4081 gtacacggcc agcggagaac tgcgggcggt gtatgaccgc agcgggacgc aggtgcgcgg
     4141 gtttgcttat gatgcggagc acgccgggcg gatggtggcg caccattatg cgggtaggcc
     4201 ggagagccgc taccggtatg atgataccgg ccgggtgacg gagctggtca acccggaggg
     4261 gctggactac cgctttgagt acgggcagga ccgtgtgacc atcacggaca gcctgaaccg
     4321 gcgggaggtg ctgtacacgg aaggcgaggg tggcctgaaa cgtgttgtga agaaggaaca
     4381 tgcggacggg agcatcaccc gcagcgagta tgatgaggcg gggaggctga aggcacagac
     4441 ggatgcggcg ggacggcgga cggagtacag cctgcatatg gcgtcgggtg cggtgacagc
     4501 ggtgacgggg ccggacggca ggacggtgcg gtatggctat aacagccagc ggcaggtgac
     4561 gtcagtgacg tacccggacg ggctgcgcag cagccgggag tatgatgaga agggaaggct
     4621 ggcggcggag acctcgcgca gcggagagac gacgcggtac agctatgatg acccggcgag
     4681 tgagctgccg acagggatac aggacgcgac gggcagtaca aaacagatgg catggagccg
     4741 ttacggtcag ctgctgacct ttacggactg ctcggggtac acgacgcggt atgagtatga
     4801 ccggtacggt caacaaatcg ccgttcaccg ggaagaaggc atcagcactt acagcagtta
     4861 taacccgcgt ggccaactgg tcagtcagaa ggatgcgcag gggcgtgaaa cccgttatga
     4921 gtacagcgcc gcaggcgacc tcaccgctat cgttgccccg gacggcagcc gcagtgagat
     4981 acagtatgat gcgtggggaa aggccgtcag caccacgcag ggcggtctga cgcgcagcat
     5041 ggggtatgac gctgccgggc gcatcaccgt gctgaccaac gagaacggca gccagtccac
     5101 gttccggtat gacccggtgg acaggctgac tgaacagcgc ggttttgacg gccggacgca
     5161 acgttaccac tatgacctga ccggaaaact cacgcagagt gaagacgagg ggcttatcac
     5221 cctctggcac tacgatgcgt cggaccgcat cacgcaccgg acggtgaacg gcgacccggc
     5281 agagcagtgg cagtatgatg agcacgggtg gctaaccacc ctcagccata ccagtgaagg
     5341 ccaccgggtg tcggtccact acggctatga cgataaaggc cgcctgacgg gtgaacggca
     5401 gacggtggag aacccggaga cgggggagat gctgtgggag catgagacgg ggcacgcgta
     5461 cagcgaacag gggctggcga cccgtcagga gccggacggt ctgccgccgg tagagtggct
     5521 gacgtatggc agcggttatc ttgcggggat gaagctgggc ggaacgccac tggtcgagta
     5581 catgcgggac cggctgcacc gtgagacggc ccgcagcttc ggcggggagg catatgaact
     5641 tgccaccgcc tggaatacca gcggccagct ccggagcagg cacctgaacc ttccgcagct
     5701 tgaccgtgac tacgactgga acgacaacgg acagctaatc cgcatcagcg gcccgcagga
     5761 gagccgggag taccgttaca gtgacacggg aaggctgacg ggcgtccaca ccacggcagc
     5821 gaacctggat atcgatatcc cgtatgcaac ggacccggca ggaaaccggc tgccggaccc
     5881 ggaactgcat ccggacagca cgctcacggc gtggccggat aaccgcatcg cggaagatgc
     5941 gcactatatc tatcgctacg atgaatacgg caggctggcg gagaagacgg accg
//
LOCUS       DAZFAB010000184         5976 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZFAB010000184 DAZFAB010000000
VERSION     DAZFAB010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5976
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.184"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..2864
                     /locus_tag="ACHJWA_004585"
     CDS             <1..2864
                     /locus_tag="ACHJWA_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO4311034.1"
                     /translation="NCTIQLKAKRNSTTVVVNTLASENPDEAGRYSMDVEYGQYSVIL
                     LVEGFPPSHAGTITVYEDSQPGTLNDFLGAMTEDDVRPEALRRFELMVEEVARNASAV
                     AQNTAAAKKSASDASTSAREAATHATDAADSARAASTSAGQAASSAQSASSSAGTAST
                     KATEASKSAAAAESSKSAAATSAGAAKTSETNAAVSQQSAATSASTATTKASEAASSA
                     RDASASKEAAKSSETSAASSASSAASSATAAGNSAKAAKTSETNAKSSETAAEQSASA
                     AAGSKTAAALSASAASTSAGQASASATAAGKSAESAASSASTATTKAGEATEQASAAA
                     SSASAAKTSETNAKASETSAESSKTAAASSASSAASSASSASASKDEATRQASAAKSS
                     ATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAEDIASAVALEDASTTKKGI
                     VQLSSATNSTSESLAATPKAVKAAYELANGKYTAQDATTAQKGIVQLSNATNSTSEML
                     AATPKSVKAAYDLANGKYTAQDATTAQKGIVQLSSATNSASETLAATPKAVKAANDNA
                     NGRVPSARKVNGKALSSDITLTPKDIGTLNSTTMSFSGGAGWFKLATVTMPQASSVVS
                     ITLIGGAGFNVGSPQQAGISELVLRAGNGNPKGITGALWQRTSTGFTNFAWVNTSGDT
                     YDIYVAIGNYATGVNIQWDYTSNASVTIHTSPAYSANKPEGLTDGTVYSLYTPSEQFY
                     PPGAPIPWPSDTVPSGYALMQGQTFDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGR
                     AVLSQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKSTNNTGAHTHSVSGTAASAGN
                     HTHSVTGASAVSQWSQNGSVHKVVSAASVNTSAAGAHTHSVSGTAASAGAHAHTVGIG
                     AHTHSVAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            2864..3445
                     /locus_tag="ACHJWA_004586"
     CDS             2864..3445
                     /locus_tag="ACHJWA_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO4311035.1"
                     /translation="MAFRMSEQPRTIKIYNLLTGTNEFIGEGDAYIPPHTGLPANSTY
                     IAPPDIPAGFVAVFNSDESSWHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSP
                     EGEFQKWNGTAWVKDAEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEEE
                     TSLLEAWKKYRVLLNRVDTSTAPDIEWPVIPEV"
     gene            complement(3565..4455)
                     /locus_tag="ACHJWA_004587"
     CDS             complement(3565..4455)
                     /locus_tag="ACHJWA_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFO4311036.1"
                     /translation="MFRHVKKLQYTVRVSEPNPGLANLLLEQFGGPQGELAAACRYFT
                     QGLSDEDAGRRDMLMDIATEELSHLEIIGSLVGMLNKGAKGALAEGTENEAELYRSLT
                     QNGNDSHITSLLYGGGPALTNSAGVPWTAAYVDTIGEVTADLRSNIAAEARAKIIYER
                     LINVTDDPGVKDTLAFLMTREAAHMLSFEKALHSIRNTFPPGKLPPLEKYKNVYYNMS
                     EGEDVRGSWNSDENFDYVSDPVSAVDGGDGKASINLSTKQEAMIKAMATRLKSHEDIN
                     PVTGAELAEGEPQTKINSKN"
     gene            complement(4474..4980)
                     /locus_tag="ACHJWA_004588"
     CDS             complement(4474..4980)
                     /locus_tag="ACHJWA_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO4311037.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAEKMLESMASRIENYPELRSRIEQ
                     HISETKNQLSQLESILDRNNISRSVIKDSMSKMAAFGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASVPIIEAILNEEKQMAEWLLNHIPDTTEQFMVRSE
                     IDGVEAKK"
     gene            complement(5017..5517)
                     /locus_tag="ACHJWA_004589"
     CDS             complement(5017..5517)
                     /locus_tag="ACHJWA_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO4311038.1"
                     /translation="MQIKSVEDIFIHLLSDTYSAEKQLTKALSKLSRSAYSDKLTAAF
                     QSHLDETHGQIERIDQVVDSEDGLKLKRIKCAAMEGLIEEANEVIESTDKNEVRDAAL
                     IAAAQKVEHYEIASYGTLVTLAEQLGYKKAAKLLKETLEEEKATDVKLTDLAFNNVNK
                     KAQDNS"
     gene            complement(5596..5778)
                     /locus_tag="ACHJWA_004590"
     CDS             complement(5596..5778)
                     /locus_tag="ACHJWA_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO4311039.1"
                     /translation="MTEHRGGAGNFAEDRQKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQNSHGGGRKSDS"
BASE COUNT         1610 a         1420 c         1443 g         1503 t
ORIGIN      
        1 agaactgcac aatccagctg aaagcaaaac gtaacagtac cacggtggtg gtgaacacgc
       61 tggcctcaga aaatccggat gaagccgggc gttacagcat ggacgttgag tacggtcagt
      121 acagcgttat tctgttggtg gaagggttcc cgccgtcaca tgccgggacc atcaccgtgt
      181 atgaagattc tcaacccggt acgctgaatg attttcttgg tgccatgact gaggatgatg
      241 tccgtccgga ggcactgcgc cgttttgagc tgatggtgga agaggtggcg cgtaacgcgt
      301 ccgcggtggc acagaacacg gcagccgcga agaagtcagc cagcgatgcc agcacatcag
      361 cccgtgaggc ggcaacccat gcgactgatg ctgcggactc agcacgcgca gccagcacgt
      421 cagccggaca ggccgcgtcg tcggctcagt cagcgtcttc cagcgcagga acggcatcaa
      481 caaaggctac tgaagcatca aaaagtgctg ccgctgcaga gtcctcaaaa agcgcggcgg
      541 ctaccagtgc cggtgcggcg aaaacgtcag aaacgaatgc ggcagtgtca caacaatcag
      601 ccgccacttc tgcatccacc gcgaccacga aagcgtcaga agctgcctcc tcagccaggg
      661 atgcgtcggc ttcaaaagag gcggcaaaat catcagaaac gagcgcagcc tcgagcgcca
      721 gtagtgcagc ctcctcggca acggcggcag gcaattccgc gaaggcggcc aaaacgtctg
      781 agacaaacgc taagtcctct gaaacggcag cagaacagag tgcctccgca gcagcaggct
      841 caaaaacagc ggctgcatta tctgccagtg ccgcgtcaac aagtgccggg caggcctcag
      901 ccagtgccac cgccgccgga aaatcggcag aaagtgccgc atcgtctgct tcaacagcca
      961 caacgaaggc tggcgaagcc actgaacagg ccagcgcagc agcgagttct gcttccgcag
     1021 cgaagacatc cgaaacgaac gcgaaagcgt cggaaaccag cgcagaatcc tcaaaaacgg
     1081 ctgccgcatc gtcagccagt tcggcggcgt catcggcatc atctgcgtct gcttcaaaag
     1141 atgaggcgac cagacaagcg tcagcagcga agagcagcgc cacgacggca tccacgaagg
     1201 cgacagaggc agctggtagt gcgacggcag cagctcagag caaaagtacg gcggaatctg
     1261 cagcaacgcg cgctgagaca gcggcaaaac gggcagagga tattgcatcc gccgtggcgc
     1321 ttgaggatgc gagcacgacg aaaaagggga tagtacagct cagcagtgcg actaacagca
     1381 cttccgagtc actggcggca acgccaaaag ccgttaaggc cgcgtatgag ctggctaacg
     1441 ggaaatacac cgcacaggat gcaacgacag cacagaaagg gatagttcag cttagcaacg
     1501 cgaccaacag cacatctgaa atgctggcgg caacgccaaa gtcggtaaag gcagcctatg
     1561 accttgctaa cgggaaatat actgctcagg acgctacgac agcacaaaaa ggaattgtcc
     1621 agctcagtag tgcaaccaac agcgcatctg aaacgcttgc cgcgacaccg aaagcagtga
     1681 aagcagctaa tgataatgcg aatggtcggg taccttctgc ccgtaaggtg aatggtaagg
     1741 cgctttcatc ggatataaca ctgacgccga aagatattgg tacgcttaac tcaacaacaa
     1801 tgtcattcag cggtggtgct ggttggttca aattagcaac ggtaaccatg ccacaggcga
     1861 gttctgttgt ttcaattacg ttgattggtg gcgcgggatt taacgtgggg tcacctcaac
     1921 aggcaggtat atctgaactt gttttgcgtg caggtaatgg taatccgaag gggattactg
     1981 gtgctttatg gcagcgcaca tcgacagggt ttacaaattt tgcctgggtc aatacatctg
     2041 gtgatactta cgatatttac gttgcaatcg gaaattatgc gactggtgta aatattcaat
     2101 gggattatac cagtaatgcc agcgtgacga ttcatacgtc accagcatat tctgctaata
     2161 agccggaagg gttaacggac ggtacagttt attcactcta tacgccatca gagcagtttt
     2221 atccgcctgg cgcaccaatc ccgtggccat cagataccgt tccgtctggc tatgccctga
     2281 tgcaggggca gacttttgac aaatctgcat acccgaaact tgcagccgct tatccgtcag
     2341 gcgtgatccc tgatatgcgt ggctggacga ttaagggcaa acctgccagt ggtcgggccg
     2401 tattgtctca ggaacaggac ggcattaaat cgcacaccca cagcgccagc gcatccagta
     2461 cggatttggg gacgaaaacc acatcgtcgt ttgattacgg cactaaatcc acgaataaca
     2521 ccggggcgca cacgcacagt gtgagcggta cagccgcaag tgccggaaac catactcata
     2581 gtgtcacagg cgcatcagca gtcagccagt ggtcacaaaa tgggtcagta cataaggtag
     2641 tgtctgcggc cagtgtgaat acaagtgctg caggagcgca cactcatagt gtcagcggca
     2701 cagctgcatc tgcaggtgct cacgcacata ctgtcggtat tggtgctcat acgcactctg
     2761 ttgcgattgg ctcacatgga cacaccatca ccgttaacgc tgcgggtaac gcggaaaaca
     2821 ctgtcaaaaa catcgcattt aactacattg tgaggcttgc ataatggcat tcagaatgag
     2881 tgaacaacca cggaccataa aaatttataa tctgctgacc ggaactaatg aatttattgg
     2941 tgaaggtgac gcatatattc cgcctcatac aggtctgcca gcaaacagta cctatattgc
     3001 accgccagat attccggctg gcttcgtggc tgttttcaac agtgatgagt catcgtggca
     3061 tctcgttgaa gatcatcggg gtaaaacggt ttatgacgtg gcttccggcg acgcgttatt
     3121 tatttctgaa ctcggctcat taccggaaaa tgttacctgg ttatcgccgg aaggggagtt
     3181 tcagaagtgg aacggcacag cctgggtgaa agatgcagaa gcagaaaaac tgttccggat
     3241 ccgggaggcg gaagaaacaa aaaacagcct gatgcaggta gccagtgagc atattgcgcc
     3301 acttcaggat gctgtagatc tggaaatcgc aacggaggaa gaaacctcat tgctggaagc
     3361 ctggaaaaag tatcgggtgt tgctgaaccg tgttgataca tcaactgcac ctgatattga
     3421 gtggcctgta atacctgaag tttaaaataa aagcccgctt aaaatatcgc gggcttcgaa
     3481 atataaatgt attttcagag gctagagctt aggatatctc agcggcaatt aacttatgag
     3541 ggatagctaa ataccagaag ataattagtt tttactattt atctttgttt gtggttctcc
     3601 ttcagcaagc tcagcgccag tgacaggatt gatgtcttca tgggatttca accttgttgc
     3661 catagctttt atcatagctt cttgcttggt cgaaagattt atcgaggcct tcccatcccc
     3721 accgtcaacc gctgaaactg gatcggaaac atagtcaaag ttttcatcac tgttccaact
     3781 acctctgacg tcttcacctt ctgacatgtt gtaatatacg tttttgtatt tctcaagtgg
     3841 tggtaatttt cctggtggaa aggtgttacg aattgaatgt aatgcttttt caaatgaaag
     3901 catgtgtgct gcttcccggg tcattaaaaa tgccagagtg tcttttactc caggatcatc
     3961 agtaacatta atgagacgtt cgtaaatgat ctttgcccga gcttcagctg caatatttga
     4021 acgaagatcg gccgtgactt cgccaatagt atcaacataa gctgcagtcc agggtactcc
     4081 agctgaatta gttaatgctg ggcctcctcc gtagaggaga gaagttatat ggctatcatt
     4141 gccattttga gttaaggaac ggtaaagctc agcttcattc tcagttcctt cagcaagtgc
     4201 tccttttgca cctttgttga gcataccaac aagagaacca ataatttcga gatgacttag
     4261 ttcttctgtt gctatgtcca tcagcatatc ccttcggcct gcatcttcat cacttaagcc
     4321 ttgagtgaag tatcggcatg ctgctgcaag ctcaccctgt ggcccaccga attgttctaa
     4381 gagtagatta gccaagcctg ggtttggctc acttacacgt actgtatatt gaagtttttt
     4441 cacgtgtcta aacataatgc ctcctcaaaa gaattatttt ttggcttcaa ctccatcaat
     4501 ttcagatctg accatgaatt gctcagtggt gtcaggaata tggttaagaa gccattcagc
     4561 catttgtttt tcttcattaa gaattgcttc aataatgggt actgaagctg tatcacccgc
     4621 atttttggct gctgcgagaa gagatgtgta acacgcaatt tcaaattgtt cgaacacata
     4681 gccactgatc gaacctttga caatttcatc agatggaaaa attccaccaa tggattgtcc
     4741 aaacgcggcc attttactca tagagtcttt aatgacagag cgcgaaatat tattacgatc
     4801 aagtatgctt tcaagctgtg aaagctggtt ctttgtttcg ctaatgtgct gctcaattct
     4861 ggaacgtaac tcaggatagt tttctatccg gctggccata gactcaagca ttttttcagc
     4921 ttgtttttcc atcgcatgtg catcacgaag ccagtcatgg taatgttcaa ttctattcat
     4981 aatctgttta tcctaaaaga aattgaagtc tatagttcag gagttatcct gagctttttt
     5041 attaacgtta ttaaatgcca ggtctgttaa tttgacatca gtagcttttt cctcttccag
     5101 tgtttctttc aggagtttag ccgctttttt atatcctaat tgctcagcga gtgttaccaa
     5161 agtgccataa ctggcaattt catagtgctc tactttttgt gctgcagcaa tcaatgcagc
     5221 atcacgtact tcatttttat cagtgctttc aattacctca ttcgcctctt cgataagtcc
     5281 ttccattgcg gcacacttaa ttcttttcaa tttaagacca tcttcagaat caacaacctg
     5341 atcgatgcgt tcaatttgac catgtgtctc atcaagatgt gactgaaaag cagcggttaa
     5401 tttatcacta tatgcagagc gactaagttt agacagtgcc tttgttagtt gtttctcagc
     5461 gctgtatgta tccgataata agtggatgaa gatatcttca acagatttaa tttgcataat
     5521 cactccagag ttttcactgc gagcccgtag gctcgcagtt agcttcagtt attcacgaat
     5581 gtagggggtg ataaattaag agtcagattt acggccgcca ccatggctat tctgaccacc
     5641 ttttttccct gcttctgatg ctctttgtgg atcgttttta aaattaccgc cactatgttg
     5701 gccgccttta cgaccagcgt cagaagcttt ttgtcgatct tcagcgaaat tacctgcacc
     5761 gccacgatgt tcggtcatat catttctcct ctgattttca aattgtaaat aacatgtgcc
     5821 ttgtcagcac ccaattaact ctagtaacaa ataagaaagt gtcaaataag agattggtat
     5881 gcgcatctta atgtgagaaa tagcaattgt aaaaatgtgt actcagtgag tataatctgt
     5941 tgatatcttg gtgattaacg gaaagtttag gttgta
//
LOCUS       DAZFAB010000185         5807 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZFAB010000185 DAZFAB010000000
VERSION     DAZFAB010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5807
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.185"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>5807
                     /gene="toxB"
                     /locus_tag="ACHJWA_004591"
     CDS             <1..>5807
                     /gene="toxB"
                     /locus_tag="ACHJWA_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toxin B"
                     /protein_id="HFO4311040.1"
                     /translation="RLDFLTNVLKLSPEVQNDFKSTVEKNKRDIDLLKNTISQKFGDR
                     FQLRDINTLESFKKPQDYFFYQQEMLLRWNYAAASDQVRINILKEYGGIYTDTDILPA
                     YSDKVSQIINEKSDDKRFFEDLKLRRIISESILSLIKGEKYSIKHDGLDETTLNQLNN
                     ILSEIEKLTIDDYFKPVETKVVRDTFKIFKRYQKWTENTWNIRGNNNFMLTHKGSKCI
                     DFILSGQKKQYLELQRIRDNISYNNLFYTTEDLKSLNNVAIGGIPAKKYLEHGLFSEY
                     RQDGTIPYVVSTLNISGPDMIMRQMKKYYKSLGRIGEVHIKDNKLSDVNFLGVYASSN
                     KDNKSFNWLNPVSVGINDITPDDESSWAVRNNDINKILFEKINCHVPEKLPTSLYYEI
                     DSRSFFQGWDNKSIKHVTEINKDLIKDINLLLTSSNIDVKLLIKLDRELYAISSKIDN
                     PLALRSIRTLQLQLANYVTSNTFEPENTINFIYDFYRKKQDDLLSAIKLFSRNDADTK
                     IIVWYNSVMEKNVFLREVISCVLRSKKVDSYINENKKNLSKEDAGALRDYAKLKMKEL
                     FSMLDDDGYKKIITTNAYIKERDKLSGIIYNIENSIISGHESFDIIRSNQHEWGDLST
                     VEQFKKFEFYVKSELSSAKSIFDDIKNKYITDPETKRNVLYHQLDSDIKERIAFLDIS
                     HYAYPGSLLEKLQLSGYVFSDINIIAEYLLASYGVSGHYSHGVVYPAPSDKLLELLRR
                     HTKSNSEWIEKITPYVYDILSDNVSNVLRPPLSEEQKKILNDIKLEISKSVSEQYFMK
                     LTEQKSSVIGIKYSVDFDRYNENLFLSLPINQNLTLPFMYRYFEMLYDIHIGIIENKA
                     NREFIYSKFSSLNLDFLINDERVLNLEGLIKKYKYLSLSEIHRTLTNSTSFADISIPL
                     LQTICPSITTIIKKTEYYGHQLTNAMTVASVVKPYDFSNLGAINSIDKSVSDVPALHT
                     IVEQAKYNLLSWNDFYNTHASIWDTIARQHKSTNIEFHPQSLLFDRDSKGKCLGLSLL
                     YLDTGGYGGGYQKLRHNIDTASTLYQTKYNDNLKLSNRDDFFLRKTQRIITMSNELGN
                     NRLKNAQLEVLELKDPILTEGILYQRRISSLLITTEYHSLALQQISSFWRVTDPNFGH
                     CDFHSLAQALTFIKNITSNRNFSSLYGSGIVKIYFSESLNNWKYIKLPLVQTGSLLRD
                     IYLTTPEKLSTSGGSLNIMGHLVPVSFIYDIGGVINGNRISESTDVKNKIRSLKINGD
                     ILQHYINTHYLSEEQTQKIKDIVDFLGIQDNTIKVKLESDIKPISEIQQPLHSILSRQ
                     KEHVKNLLSGLLDEFSNKLRKQGLSLKTNVLSVNNFKESKINSDTVEVTVTDLQGRLY
                     RVDIDTRVIGLTFKEGINSLSEALEHMNIDAIMSVIGLVQYARMIKMNDNISAIDHAG
                     AVSDIKNIVDKFLGGILTLTNNRVYNPGGVSGASLEGFISSGLEVCASRMGGTAGRYL
                     SNVAKVIKLPLLDIGINIWSLYDSSLNHAKATTQIEYISTAIDVSFSSINTALSIGAI
                     AYPPLAIAIVPITIFSHEVKNYAVYVNQINERHKLWLEAEKYLDNGSAKVLSINKATG
                     IIDLSNNQVLGNIYLDMRENPPILHGEKSYNSGKNIGSHPDMTDREIMESRAYNFACT
                     KLSDAGEPDIFGWGDKEICNSMELSESQLANGYSNRQWPSQIPVIPEGIYNTVYLGYG
                     ETLRANTEVTLSSTGYFYEIARAYTDDELSEPLLTVCNQHSHVIGGKEPLTIIIPAIE
                     HGMLGSNLHMIERFKNYNFSISGGKGGIKLMVGGIGDYNIECTPGVRNIISFEQLSRD
                     FNLDLDLSDGRKQNFNFHHPSGFYSGKVMSITQKGINAVVGTKLGYDKIRGNNLDNTF
                     SLGSGGGVIYSGGGSNTYFVPATLQDNLHIYISEK"
BASE COUNT         2141 a          835 c         1035 g         1796 t
ORIGIN      
        1 cagacttgac tttttaacta acgttttaaa actttcacca gaagttcaaa atgactttaa
       61 atctacagta gagaaaaaca aaagagatat agacttatta aaaaatacaa tatctcaaaa
      121 atttggggat agatttcaac tgagagatat taacacattg gagtcattta aaaaaccaca
      181 agattatttt ttttatcaac aagagatgct attaagatgg aactacgccg ctgcatcaga
      241 tcaggtaaga attaatatat tgaaagagta tggagggatt tataccgata cagatattct
      301 tcctgcatat tcagataaag tatcacagat aataaatgaa aaaagtgatg ataagaggtt
      361 ttttgaagat ttaaaactaa gaaggattat atcagaatct attctgtctc tgataaaagg
      421 ggaaaagtac tcaattaaac atgatggatt agatgaaaca acactaaatc agttaaataa
      481 catattgagt gaaatagaaa aactgacgat agatgattat tttaaacccg ttgagacaaa
      541 agttgtcaga gatacattta aaatttttaa aagatatcaa aaatggacag aaaacacctg
      601 gaatattaga gggaataata atttcatgtt aactcacaag ggaagtaaat gtattgattt
      661 tattctatca ggacagaaaa aacagtattt agaactccaa cgcatcagag ataatataag
      721 ttataataat ctattttata ctacagagga tctaaagtct ttaaataatg tggcaatagg
      781 aggaatacct gcaaagaaat atttagaaca cggtttgttt tctgaatata gacaggacgg
      841 tacaattcct tatgtggtta gcactttaaa tatatctggc ccagacatga ttatgcgaca
      901 aatgaaaaaa tactataaat ctcttgggcg cataggtgaa gtgcatatta aagataataa
      961 attgagtgat gttaattttt tgggtgtata tgcaagttca aataaagaca ataaaagttt
     1021 taactggcta aatccagtga gtgttggtat taatgatatt acaccagatg atgaaagctc
     1081 atgggctgtc agaaataatg atataaataa aattctattc gaaaaaataa actgccatgt
     1141 acccgaaaaa ctacctacct ccctatacta tgaaatcgat tcgcggtctt tttttcaggg
     1201 gtgggataat aaaagcataa agcatgttac agagattaat aaagacctca tcaaagatat
     1261 taatttatta ttaacatcgt ctaatattga tgttaaactt ttaataaaac tggatcgtga
     1321 attatatgcg attagcagca aaatagataa tccgttggcc ttgcgctcta taagaacctt
     1381 acagctacaa ttagctaatt acgtaacttc gaatacattt gaacctgaaa acacaattaa
     1441 ttttatttat gatttttata gaaaaaaaca agatgactta ctcagcgcaa taaaattatt
     1501 ttcgcgtaat gatgccgata caaaaattat tgtctggtac aattccgtaa tggaaaaaaa
     1561 tgtgtttctc cgagaggtta tatcatgtgt tctacgaagt aaaaaagtag atagttatat
     1621 aaatgaaaac aaaaaaaact tatctaagga ggatgccgga gctttaagag attatgcaaa
     1681 attaaaaatg aaagaactat tttcgatgtt agatgatgat ggttataaaa aaatcataac
     1741 tacaaatgca tatattaaag agagggataa actatcaggc attatataca acatcgaaaa
     1801 ttcgattata tcgggtcatg aatcatttga tataatacga agcaatcaac atgagtgggg
     1861 ggatttatca acggtagagc aatttaaaaa atttgagttt tatgtgaaaa gtgaattatc
     1921 ttcggctaag tcaattttcg atgatataaa aaacaaatat ataacagatc ctgaaacaaa
     1981 acgaaacgta ctctaccatc agttagatag tgatattaaa gagcgaatag catttctcga
     2041 tatatcccac tatgcatatc cgggtagttt attagaaaaa ttacaattaa gtggctacgt
     2101 tttttctgat atcaacataa ttgcagaata cttattggct agttacggag tttctggaca
     2161 ttattctcac ggcgtggtat atccagctcc atctgataaa ttacttgagc tattaagaag
     2221 acatacaaag agcaattctg agtggataga aaaaataacc ccatatgttt atgatatatt
     2281 atccgataat gtatccaacg tcttgcgccc ccctttatca gaagaacaga aaaaaatatt
     2341 gaatgatata aaattagaaa ttagtaaatc tgtatcagaa cagtatttca tgaagttgac
     2401 agagcaaaaa tcatcagtta ttggtattaa atattctgtt gattttgaca gatacaatga
     2461 gaatttgttt ttgtctttac ctataaatca aaatttaacg ctgcctttta tgtatcgcta
     2521 cttcgagatg ctatatgaca ttcacattgg aataatagaa aacaaggcaa acagggagtt
     2581 tatatatagt aagttttcat ctttaaacct ggattttttg attaatgacg agagagtttt
     2641 gaatcttgaa ggtttaataa aaaaatataa atatttatcg ctgagtgaaa tacatagaac
     2701 tttaacaaat agtacttcat ttgcagatat ttctatacca ttattgcaga ccatatgtcc
     2761 cagcataaca acaataataa aaaaaacaga atattatggg catcagttaa caaatgctat
     2821 gactgttgct tcagtggtta aaccatatga ctttagcaat cttggtgcca taaatagtat
     2881 agataaatcc gtttctgatg tgcctgcatt gcatacaatt gttgaacaag caaaatataa
     2941 tttactttca tggaatgatt tttataacac tcatgcgtca atatgggata caatagcaag
     3001 acagcataaa agtacaaata tcgaatttca cccacaaagt cttctttttg atagagattc
     3061 gaaaggaaag tgccttggtt tatctcttct gtatcttgat acaggggggt acggaggcgg
     3121 atatcaaaaa ctgaggcaca atattgacac tgccagcaca ttatatcaaa caaaatacaa
     3181 tgacaatcta aaattatcta accgtgatga tttcttttta agaaaaacac agcgtatcat
     3241 tacaatgtcc aatgagttag gtaataacag gttaaaaaat gcacagttag aggttctgga
     3301 attgaaagac cctatactaa ctgaaggcat attataccag cgtcgtatat catctctttt
     3361 aataacaaca gaatatcatt cacttgcatt acaacaaata tcttcttttt ggagagtaac
     3421 tgatcctaat tttggtcatt gtgactttca ttccttagca caggctttaa cgtttataaa
     3481 aaatataacg agcaatcgta acttttcatc tttatatgga agtggcattg ttaaaattta
     3541 cttcagtgaa tctcttaata attggaagta tattaagctc ccccttgttc agactggttc
     3601 tttgttaaga gacatatatt taacaacccc agaaaaatta tccacaagcg ggggttcatt
     3661 aaatattatg gggcatctcg tgcctgtcag ttttatttat gatatcggag gggttattaa
     3721 tggtaacaga ataagtgaaa gtacagatgt gaaaaataaa attaggagct taaaaataaa
     3781 tggggatatt ttacaacatt atataaatac gcattatttg tcagaggagc aaactcagaa
     3841 gataaaagat attgtggatt ttttaggtat tcaggataac acaataaaag ttaagcttga
     3901 aagtgatatt aaacctataa gtgaaatcca acagcccctt cattccattc tttcacggca
     3961 gaaagaacac gtaaagaatt tactatctgg attgcttgat gagttttcaa ataagttaag
     4021 aaagcaaggt ctttcactta aaacaaatgt tctttcagtt aataacttca aagaatcaaa
     4081 gataaatagt gatacagtgg aggttacagt aacagatctg caaggaagac tttatcgtgt
     4141 tgatatagat acaagagtta ttggcttaac ttttaaagag gggataaata gtttatccga
     4201 agcactcgaa cacatgaata tcgatgcgat tatgtctgtt attggcttgg ttcaatatgc
     4261 acgaatgata aaaatgaatg acaatattag tgcaatagat catgcaggag cagtatcaga
     4321 tataaaaaat attgtagaca aatttttagg cggaattttg actcttacta ataatcgcgt
     4381 atataatcct ggcggtgtat ctggtgcctc gcttgaagga tttatatctt ctgggttgga
     4441 ggtgtgtgct tcaagaatgg gtggcaccgc agggcgttat cttagcaatg tggcaaaagt
     4501 aattaaatta cctttgctag atataggcat aaatatttgg tcattatatg attcttcatt
     4561 aaatcatgct aaagccacca cacaaattga atatatatca acagcaatag atgtatcttt
     4621 ttcatctata aatacagctt tgtcaatagg cgcaatcgca tatcctcctt tggcaatagc
     4681 tatagttccg ataactattt tttctcatga agtaaaaaac tatgctgttt atgtgaatca
     4741 aattaatgag cgccataagt tatggctcga agctgaaaag tatctggaca atggaagtgc
     4801 aaaggttcta agcattaaca aagccacagg cataatcgat ttatcaaata atcaggtgtt
     4861 aggtaatatt tatttagata tgagagaaaa tcctccaata ttacacggtg agaaatcata
     4921 taatagcgga aagaatattg gtagtcatcc tgatatgact gacagagaga ttatggagtc
     4981 tagagcatac aattttgcat gcacaaaact ctcagatgca ggtgaacccg atattttcgg
     5041 ttggggagat aaggaaatat gtaattcaat ggaattatca gaatcacaat tagctaatgg
     5101 gtattctaac cgccaatggc cttctcagat tccagttatt ccagaaggaa tttataatac
     5161 agtatatctt ggatatggag aaacattgcg agctaatact gaagttacat tgagtagcac
     5221 tggttacttt tacgagattg caagggcata tactgatgat gaactatctg agccattatt
     5281 aacagtatgt aaccaacata gtcatgttat tggtggaaaa gaaccattaa caattattat
     5341 tcccgctatt gaacatggta tgcttggctc taatctacat atgattgaac gttttaaaaa
     5401 ctataatttc tcgatatcag gagggaaggg agggataaaa ttaatggttg gtggtattgg
     5461 tgattacaac atagaatgta cccccggtgt tagaaatatt atatcgtttg aacaattatc
     5521 acgtgacttc aatctggatt tggatttatc agatgggagg aaacaaaatt ttaattttca
     5581 tcatccctca ggtttttatt caggaaaagt tatgagcata acgcaaaagg gtataaatgc
     5641 ggttgttggt actaaactgg gatatgataa gattcgtggt aataatcttg ataatacctt
     5701 ttctcttggg agtggaggag gtgttatata ttctggagga gggagcaata cctatttcgt
     5761 tcccgccaca ctacaggata atttacacat atatatatca gaaaaat
//
LOCUS       DAZFAB010000186         5777 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZFAB010000186 DAZFAB010000000
VERSION     DAZFAB010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5777
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.186"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            18..1007
                     /gene="ldhA"
                     /locus_tag="ACHJWA_004592"
     CDS             18..1007
                     /gene="ldhA"
                     /locus_tag="ACHJWA_004592"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO4311041.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            1118..1540
                     /gene="hslJ"
                     /locus_tag="ACHJWA_004593"
     CDS             1118..1540
                     /gene="hslJ"
                     /locus_tag="ACHJWA_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFO4311042.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTCDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(1537..1803)
                     /locus_tag="ACHJWA_004594"
     CDS             complement(1537..1803)
                     /locus_tag="ACHJWA_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFO4311043.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            2077..5601
                     /gene="nifJ"
                     /locus_tag="ACHJWA_004595"
     CDS             2077..5601
                     /gene="nifJ"
                     /locus_tag="ACHJWA_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFO4311044.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPAFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT         1445 a         1487 c         1491 g         1354 t
ORIGIN      
        1 tcactggaga aagtcttatg aaactcgccg tttatagcac aaaacagtac gacaagaagt
       61 acctgcaaca ggtgaacgag tcctttggct ttgagctgga attttttgac tttctgctga
      121 cggaaaaaac cgctaaaact gccaatggct gcgaagcggt atgtattttc gtaaacgatg
      181 acggcagccg cccggtgctg gaagagctga aaaagcacgg cgttaaatat atcgccctgc
      241 gctgtgccgg tttcaataac gtcgaccttg acgcggcaaa agaactgggg ctgaaagtag
      301 tccgtgttcc agcctatgat ccagaggccg ttgctgaaca cgccatcggt atgatgatga
      361 cgctgaaccg ccgtattcac cgcgcgtatc agcgtacccg tgacgctaac ttctctctgg
      421 aaggtctgac cggctttact atgtatggca aaacggcagg cgttatcggt accggtaaaa
      481 tcggtgtggc gatgctgcgc attctgaaag gttttggtat gcgtctgctg gcgttcgatc
      541 cgtatccaag tgcagcggcg ctggaactcg gtgtggagta tgtcgatctg ccaaccctgt
      601 tctctgaatc agacgttatc tctctgcact gcccgctgac accggaaaac taccatctgt
      661 tgaacgaagc cgccttcgat cagatgaaaa atggcgtgat gatcgtcaat accagtcgcg
      721 gtgcattgat tgattctcag gcagcaattg aagcgctgaa aaatcagaaa attggttcgt
      781 tgggtatgga cgtgtatgag aacgaacgcg atctgttctt tgaagataaa tccaacgacg
      841 tgatccagga tgacgtattc cgtcgcctgt ctgcctgcca caacgtgctg tttaccgggc
      901 accaggcatt cctgacagca gaagctctga ccagtatttc tcagactacg ctgcaaaact
      961 taagcaatct ggaaaaaggt gaaacctgcc cgaacgaact ggtttaatct tgccgctccc
     1021 ctgcaatcca ggggagctga ttcagataat ccccaatgac ctttcattct ctattcttaa
     1081 aatagtcctg agtcagaaac tgtaattgag aaccacaatg aagaaagtag ccgcgtttgt
     1141 tgcgctaagc ctgctgatgg cgggatgtgt aagtaatgac aaaattgctg ttacgccaga
     1201 acagctacag catcatcgct ttgtgctgga aagcgtaaac ggtaagcccg tgacctgcga
     1261 taaaaatccg ccagaaatca gctttggtga aaaaatgatg atttccggca gcatgtgtaa
     1321 ccgctttagc ggtgaaggca aactgtctaa tggtgaactg acagccaaag ggctggcaat
     1381 gacccgtatg atgtgcgcta acccgcagct taatgaactc gataacacca ttagcgaaat
     1441 gctgaaagaa ggtgcacaag tggatctgac cgcgaaccag ttaacgctgg cgaccgcgaa
     1501 acagacatta acttataagc tggcggattt aatgaattaa tagctgccac agctcccggc
     1561 ggcaagtgac tgttcgctac agcgtttgcc gttgggtaat gcacacatcc caatcgccgt
     1621 accatccagt tgacgggcaa cagaaagcga accgccgatc attgcacaat ttgcttctcc
     1681 actactggac atcgacgctt ttaaacctgg cgctacgtgc gccgcagtcg cctgctgaac
     1741 aggctcacta ctacacgccg acaacaataa agcggcacac cctacccaaa acgctgctcg
     1801 catctcttct tcctctgatc ttcaagccaa acgacaccgc cataaataat aggcagcaca
     1861 gagggcgacg tcgagagctg tcctgcgcgt tgccccgcat ttttactttt ttatggctat
     1921 ttttttgccc tctgtttgat caaaacattc attacgctga tgtgggggac acaaaagcga
     1981 aaatgcagaa gaaagccatt tgctaaaatt gaaagattac tactgggcgc gcagcaattt
     2041 cgtgcgcccc tcatttgcgc aatgtaaggg tgtcatatga ttactattga cggtaatggc
     2101 gcggttgctt cggtggcgtt tcgcaccagt gaagttatcg ccatctaccc tattaccccc
     2161 agttccacga tggcagaaca ggctgatgcc tgggccggaa acggcttgaa gaacgtttgg
     2221 ggagacacac cacgcgtggt tgaaatgcag tcggaagcgg gtgctatcgc taccgtgcat
     2281 ggcgctttgc agacgggtgc cctttcaaca tcgtttacgt catcgcaggg tttgctgctg
     2341 atgatcccga cgctgtacaa actggcaggc gaactcacac cgtttgtcct gcatgtagcg
     2401 gcacgtaccg ttgccacaca tgcactctct atttttggcg atcattccga cgtcatggcg
     2461 gtgcgccaga cgggttgcgc gatgttgtgt gcggcaaacg tccaggaagc gcaagacttt
     2521 gctctcattt cgcacatcgc gacgctgaaa agccgcgtgc catttattca tttctttgat
     2581 ggtttccgta cgtcccacga aatcaataaa attgtcccgc tggccgatga cactattctt
     2641 gatctcatgc cgcaggccga aattgatgct catcgcgccc gggcgctcaa cccggaacat
     2701 cccgtgatcc gcggtacgtc agccaatcct gacacttatt tccagtctcg cgaagccacc
     2761 aacccatggt acaacgcggt ctatgaccat gttgaacagg cgatgaatga tttctctgcc
     2821 gcgacaggtc gtcagtatca gccgtttgaa tattacgggc atccgcaagc ggaacgggtg
     2881 attatcctga tgggctctgc cattggcacc tgtgaagaag tggttgatga attgctaacc
     2941 cgtggcgaaa aagttggcgt gctgaaagtt cgcctgtacc gtcccttctc cgccaaacac
     3001 ttgctgcaag ctctgccggg atccgtacgc agcgtggcgg tactggacag aaccaaagaa
     3061 cccggtgccc aggcagaacc gctctatctg gatgtaatga ccgcactggc agaagccttt
     3121 aataatggcg agcgcgaaac tctgccccgt gtcattggtg ggcgctatgg tctttcatcc
     3181 aaagaatttg gcccggactg tgtactggcg gtatttgccg agctcaacgc ggctaaaccg
     3241 aaagcgcgct ttacggttgg tatttacgat gatgtgacca atctgtcact gccgttgccg
     3301 gaaaacaccc tgccaaactc ggcgaaactg gaagccttgt tttatggcct tggtagtgat
     3361 ggcagcgttt ccgcgaccaa aaacaatatc aagattatcg gtaattccac gccgtggtac
     3421 gcacagggct attttgttta cgactccaaa aaggcgggcg gcctgacggt ttctcacctt
     3481 cgcgtgagcg aacagccgat tcgttccgct tatctcattt cccaggctga ttttgttggc
     3541 tgccaccagt tgcagtttat cgataaatat cagatggctg agcgtttaaa acctggcggc
     3601 attttcctgc tcaacacgcc gtacagcgca gatgaagtgt ggtcgcgctt gccgcaggaa
     3661 gttcaggccg tgctaaatca gaaaaaagcg cgcttctatg tgattaacgc ggcgaaaatc
     3721 gcccgcgaat gtggcctggc ggcccgtatt aataccgtca tgcagatggc ttttttccat
     3781 ctgacacaaa ttctgcctgg cgatagcgcc ctcgcagaat tgcagggtgc gattgccaaa
     3841 agttacagta gcaaaggtca ggatctggtg gaacgcaact ggcaggctct ggcgctggcg
     3901 cgtgaatccg tagaagaagt tccgttgcaa ccggtaaatc cgcacagcgc caatcgaccg
     3961 ccagtggttt ccgatgccgc ccctgctttc gtgaaaaccg taaccgctgc gatgctcgcc
     4021 gggcttggtg atgccctccc cgtttcggcg ctgccgccag acggcacctg gccgatgggc
     4081 accactcgct gggaaaaacg caatatcgcc gaagagatcc ccatctggaa agaggaactc
     4141 tgtacccaat gtaaccactg cgtcgccgct tgcccacact cagctattcg cgcaaaagtg
     4201 gtaccgcctg aagcgatgga aaacgcccct gccagcctgc attcgctgga tgtgaaatcg
     4261 cgtgatatgc gcgggcagaa atatgtcttg caggtggctc cggaagattg caccggttgt
     4321 aacctgtgcg tcgaagtttg cccggcgaaa gaccgtcaga atccagagat taaagccatc
     4381 aatatgatgt cgcgcctgga acatgtcgaa gaagagaaaa tcaattacga tttcttcctc
     4441 aacctgccag aaatcgaccg tagcaaactg gaacgtattg atattcgcac atcgcagctg
     4501 attacaccgc tgtttgaata ttccggtgct tgctccggtt gtggcgagac gccgtatatt
     4561 aaattgctga ctcagctcta tggtgaccgg atgttgatcg ctaacgccac cggctgttct
     4621 tcaatttatg gcggtaacct gccctctaca ccgtatacca ccgatgccaa cggtcgtggg
     4681 ccggcatggg cgaactctct atttgaagat aatgccgaat ttggccttgg tttccgcctg
     4741 acggtcgatc aacaccgtgt ccgcgtgctg cgtctgctgg atcaatttgc cgataaaatc
     4801 ccggcggaat tactgacggc gttgaaatca gacgccacgc cagaggttcg tcgtgaacag
     4861 gttgcagctt tacgccagca actcaacgat gttgccgaag cacatgaact gctgcgtgac
     4921 gcagatgcac tggtggaaaa atcaatctgg ctgattggtg gcgacggctg ggcttacgat
     4981 atcggctttg gcggtctgga tcatgtattg agtttgacgg aaaacgtcaa cattctggtg
     5041 ctggatacgc aatgctattc caacaccggt ggtcaggcgt cgaaagcgac accgctgggt
     5101 gcagtaacta aatttggcga gcacggcaaa cgtaaagcgc gtaaagatct tggcgtcagt
     5161 atgatgatgt acggtcatgt ttatgtggct cagatttctc tcggcgcgca gctgaaccag
     5221 acggtgaaag cgattcagga agcggaagcg tatccgggac catcgctgat cattgcttat
     5281 agcccgtgtg aagagcatgg ttacgatctg gcactcagcc atgaccagat gcgccaactc
     5341 acagctaccg gcttctggcc gctatatcgc ttcgatccgc gtcgtgccga tgaaggcaaa
     5401 ctgccgctgg cgctggattc acgcccgccg tcagaagcac tggaagaaac gttacttcac
     5461 gagcaacgtt tccgtcgtct gaattcgcag cagccagaag tggcagaaca gttatggaaa
     5521 gatgctgcag ctgatttgca aaaacgctat gacttcctgg cacaaatggc cggaaaagcg
     5581 gaaaaaagca acaccgatta aatgctctgg ataaggatta tccaattcta aaaaaaagcc
     5641 cggacgactg ttcgggcttg tctttttata tgttggaaaa tcagtggcaa tgcaatggcc
     5701 caacagaaaa taaaatctgt gacaataaag gcatataacc cgcgcagaat aacgtataga
     5761 aaattaaaag tataatt
//
LOCUS       DAZFAB010000187         5738 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZFAB010000187 DAZFAB010000000
VERSION     DAZFAB010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5738
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.187"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            50..940
                     /gene="prpB"
                     /locus_tag="ACHJWA_004596"
     CDS             50..940
                     /gene="prpB"
                     /locus_tag="ACHJWA_004596"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFO4311045.1"
                     /translation="MSLHSPGKAFRAALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYHYEEKLDDLFVRSQAK"
     gene            1096..2265
                     /gene="prpC"
                     /locus_tag="ACHJWA_004597"
     CDS             1096..2265
                     /gene="prpC"
                     /locus_tag="ACHJWA_004597"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFO4311046.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVESKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            2299..3750
                     /gene="prpD"
                     /locus_tag="ACHJWA_004598"
     CDS             2299..3750
                     /gene="prpD"
                     /locus_tag="ACHJWA_004598"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HFO4311047.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTGMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVSIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPVFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRARLEQMPVNEYLDLYVI"
     gene            3790..5676
                     /gene="prpE"
                     /locus_tag="ACHJWA_004599"
     CDS             3790..5676
                     /gene="prpE"
                     /locus_tag="ACHJWA_004599"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HFO4311048.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLNQIRQ
                     AMEE"
BASE COUNT         1276 a         1564 c         1676 g         1222 t
ORIGIN      
        1 gctttgtcgt tatcaacgaa aataacaagt tgataacaaa ggatgggcta tgtctctaca
       61 ctctccaggt aaagcgtttc gcgctgcact gactaaagaa aatccattgc agattgttgg
      121 caccattaac gccaaccatg cgctgttggc gcagcgtgcc ggatatcagg caatttatct
      181 ctccggcggt ggcgtggcgg cagggtcgct ggggctgccc gatctcggta tttctactct
      241 tgatgacgtg ttgacagata ttcgccgtat caccgacgtt tgttcgctgc cgctgctggt
      301 ggatgcggat atcggttttg gttcttctgc ttttaacgtt gcgcgtacgg tgaaatcgat
      361 gattaaagcc ggtgcggcgg gattgcatat tgaagatcag gttggtgcaa aacgctgtgg
      421 tcatcgcccg aacaaagcga tcgtctcgaa agaggagatg gtggatcgga tccgcgcggc
      481 ggtggatgcg aaaaccgatc ctgattttgt gatcatggcg cgcaccgatg cgctggcggt
      541 agaggggctg gatgcggcga tcgagcgtgc gcaggcctat gttgaagcgg gtgccgagat
      601 gttgttcccg gaggcgatta ccgaactcgc catgtaccgc cagtttgccg atgcggtgca
      661 ggtgccaatc ctcgccaaca ttaccgaatt tggcgccacg ccgctgttta ccaccgacga
      721 attacgcagc gctcatgtcg cgatggcgct gtacccgctt tcagcgtttc gcgccatgaa
      781 ccgcgccgcc gaacatgtct acaacgtcct gcgccaggaa ggcacgcaga aaagcgtcat
      841 cgacaccatg cagacccgca acgagctgta cgaaagcatc aactactacc actacgaaga
      901 gaagctcgac gacctgtttg tccgtagcca ggcgaaataa aaaacgcccg ttggttgtat
      961 tcgacaaccg atgcctgatg cgccgctgac gcgacttatc aggcctacgg ggtgaactgt
     1021 aggtcggata agacgcatgg cgtcgcatcc gacaacaatc tcgaccctac aaatgataac
     1081 aatgacgagg acaatatgag cgacacaacg atcctgcaaa acagtaccca tgtcattaaa
     1141 ccgaaaaaat ctgtggcgct ttctggcgtt ccggcgggca atacggcgct ctgcaccgtg
     1201 ggtaaaagtg gcaatgactt gcattaccgt ggctacgata ttcttgatct ggcggaacat
     1261 tgcgaatttg aagaagtggc gcatctgctg atccacggca aactgccgac ccgtgacgaa
     1321 ctcgccgcct acaaaacgaa actgaaagcc ctgcgcggtt taccggctaa cgtgcgtacc
     1381 gtgctggaag ccttaccggc ggcgtcacac ccgatggatg ttatgcgcac cggcgtttcc
     1441 gcgctcggct gcacgctgcc agaaaaagag gggcataccg tctctggcgc gcgggatatt
     1501 gccgacaaac tgctggcgtc acttagctcg attcttctct actggtatca ctacagccac
     1561 aacggcgaac gcatccagcc ggaaaccgat gacgactcca tcggtggtca cttcctgcat
     1621 ctgctgcacg gcgaaaagcc gtcgcaaagc tgggaaaagg cgatgcatat ttcgctggtg
     1681 ctgtacgccg aacacgagtt taacgcctcc acctttacca gccgggtgat tgcgggcact
     1741 ggctctgata tgtattccgc gattattggc gcgattggtg cactgcgcgg gccgaagcac
     1801 ggcggggcga atgaagtgtc gctggagatc cagcaacgct acgaaacgcc ggacgaagcc
     1861 gaagccgata tccgcaagcg ggtggaaagc aaagaagtgg tcattggttt tggtcatccg
     1921 gtttatacca tcgccgaccc gcgccaccag gtgattaaac gtgtggcgaa gcagctctcg
     1981 caggaaggcg gctcgctgaa gatgtacaac atcgccgatc gcctggaaac ggtgatgtgg
     2041 gagagcaaaa agatgttccc caatctcgac tggttctccg ctgtttccta caacatgatg
     2101 ggcgttccca ccgagatgtt cacaccactg tttgttatcg cccgcgtcac cggctgggcg
     2161 gcgcacatta tcgaacaacg tcaggacaac aaaattatcc gtccttccgc caattatgtt
     2221 ggaccggaag accgccagtt tgtcgcgctg gataagcgcc agtaaacctc tacgaataac
     2281 aataaggaaa cgtacccaat gtcagctcaa atcaacaaca tccgcccgga atttgatcgt
     2341 gaaatcgttg atatcgtcga ttacgtcatg aactacgaaa tcagctccaa agtagcctac
     2401 gacaccgcac attactgtct gctcgacacg ctcggctgcg gtcttgaagc tctcgaatac
     2461 ccagcctgta aaaaactgct ggggccaatt gtccccggca ccgtcgtacc taacggcgtg
     2521 cgcgtccccg gaactcagtt ccagctcgac cccgtccagg cggcatttaa catcggcgcg
     2581 atgatccgct ggctcgattt caacgatacc tggcttgcgg cagagtgggg ccatccttcc
     2641 gacaatctcg gcggcattct ggcaacggcg gactggcttt cacgcaacgc ggtcgccagc
     2701 ggcaaagcgc cgttgaccat gaaacaggtg ctgaccggaa tgattaaagc ccatgaaatt
     2761 cagggctgca tcgcgctgga aaactccttt aaccgcgtcg gcctcgacca cgttctgtta
     2821 gtgaaagtgg cttccaccgc cgtggtcgcc gaaatgcttg gcctgacccg cgaggaaatt
     2881 ctcaacgctg tttcgctggc gtgggtggac ggtcagtcgc tgcgcaccta tcgccatgcg
     2941 ccgaacaccg gcacgcgtaa atcctgggcg gcgggcgatg ccacttcccg cgcggtacgt
     3001 ctggcactga tggcgaaaac gggcgaaatg ggttacccgt cagccctgac tgcgcctgtg
     3061 tggggcttct acgacgtctc ctttaaaggt gaatcgttcc gcttccagcg tccgtacggt
     3121 tcttacgtga tggaaaatgt gctgttcaaa atctccttcc cggcggagtt ccactcccag
     3181 acggcagttg aagcagcgat gacgctctat gaacagatgc aggcagcagg caaaacggcg
     3241 gcagatatcg aaaaagtgtc catccgcacc cacgaagcct gtattcgcat catcgacaaa
     3301 aaggggccgc tcaataaccc ggcagaccgc gaccactgca ttcagtacat ggtggcgatc
     3361 ccactgctat tcgggcgctt aacggcggca gattacgagg acaacgttgc gcaagataaa
     3421 cgcattgacg ccctgcgcga gaagatcaat tgctttgaag atccggtatt taccgctgac
     3481 taccacgacc cggaaaaacg cgccatcgcc aatgccatta cccttgagtt caccgacggc
     3541 acacgatttg aagaagtggt ggtggagtac cccattggtc atgctcgccg ccgtcaggat
     3601 ggtattccga aactggtcga taaattcaaa atcaatctcg cgcgccagtt cccgactcgc
     3661 caacagcagc gcattctgga ggtttctctc gacagagctc gcctggaaca gatgccggtc
     3721 aatgagtatc tcgacctgta cgtcatttaa gtaaacggcg gtaaggcgta agttcaacag
     3781 gagagcatta tgtcttttag cgaattttat cagcgttcga ttaacgaacc ggagcagttc
     3841 tgggccgagc aggcccggcg tattgactgg cagacgccct ttacgcaaac gctcgatcac
     3901 agcaatccgc cgtttgcccg ttggttttgt gaaggccgaa ccaacttgtg ccacaacgct
     3961 atcgaccgct ggctggagaa acaaccagag gcgctggcgc tgattgccgt ctcttcggaa
     4021 acagaagaag agcgcacctt tacctttcgc cagcttcatg acgaagtaaa cgcggtggcg
     4081 tcaatgctgc gctcgctggg cgtgcagcgt ggcgatcggg tgctggtgta tatgccgatg
     4141 attgccgaag cgcatattac tctgctggcc tgcgcgcgca ttggcgctat tcactcggtg
     4201 gtgtttgggg gatttgcctc gcacagcgtg gcggcgcgaa ttgatgatgc taaaccggtg
     4261 ctgattgtct cggctgatgc cggagcgcgc ggtggcaaaa tcattcccta taaaaaattg
     4321 ctcgacgatg cgataagtca ggcgcagcac cagccacgcc atgttttgct ggtggatcgc
     4381 gggctggcga aaatggcacg cgtcagcggg cgggatgtcg atttcgcgtc gttgcgccat
     4441 caacacatcg gcgcgcgggt accggtggcg tggctggaat ccaacgaaac ctcctgcatt
     4501 ctctacacct ccggcacgac cggcaaacct aaaggcgtgc agcgtgacgt tggcggatat
     4561 gcggtggcgc tggcgacctc gatggacacc atttttggcg gcaaagcggg cggtgtgttc
     4621 ttttgtgctt cggatatcgg ctgggtggtg gggcattcgt atatcgtcta cgcgccgctg
     4681 ctggcgggga tggcgactat cgtttacgaa ggattaccga cctggccgga ctgcggcgtg
     4741 tggtggaaaa tcgtcgagaa atatcaggtt agccggatgt tctcagcgcc gaccgccatt
     4801 cgcgtgctga aaaaattccc taccgctgaa attcgcaaac acgatctctc gtcgctggaa
     4861 gtgctctatc tggctggaga accgctggac gagccgaccg ccagttgggt gagcaatacg
     4921 ctggatgtgc cggtcatcga caactactgg cagaccgaat ccggctggcc gattatggcg
     4981 attgctcgcg gtctggacga caggccgacg cgtctgggaa gccccggcgt gccgatgtat
     5041 ggctataacg tgcagttgct taatgaagtc accggcgaac cgtgtggcgt caacgagaaa
     5101 gggatgctgg tggtggaagg gccgctgccg ccggggtgta ttcagaccat ctggggcgac
     5161 gacgaccgct ttgtgaagac gtactggtcg ctgttttccc gcccggtgta cgccaccttt
     5221 gactggggca tccgtgacgc tgacggttat cactttattc tcgggcgcac tgacgatgtg
     5281 attaacgttg ccgggcatcg gctggggacg cgcgagattg aagagagtat ctccagtcat
     5341 ccgggcgttg ccgaagtggc ggtggttggg gtgaaagatg cgctgaaagg gcaggtggcg
     5401 gtggcgtttg tcattccgaa agagagcgac agtctggaag atcgtgatgt ggcgcattcg
     5461 caagagaagg cgattatggc gctggtggac agccagattg gcaactttgg ccgcccggcg
     5521 cacgtctggt ttgtctcgca attgccaaaa acgcgatccg gaaaaatgct gcgccgcacg
     5581 atccaggcga tttgcgaagg acgcgatcct ggggatctga cgaccattga tgatcctgcg
     5641 tcgttgaatc agatccgcca ggcgatggaa gagtaaggta ttgtcggatg cgtcgcgtgg
     5701 cgcatccgac agttgtgcgc agatgcctga tgcgacgc
//
LOCUS       DAZFAB010000188         5633 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZFAB010000188 DAZFAB010000000
VERSION     DAZFAB010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5633
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.188"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(182..850)
                     /gene="artM"
                     /locus_tag="ACHJWA_004600"
     CDS             complement(182..850)
                     /gene="artM"
                     /locus_tag="ACHJWA_004600"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFO4311049.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(850..1566)
                     /gene="artQ"
                     /locus_tag="ACHJWA_004601"
     CDS             complement(850..1566)
                     /gene="artQ"
                     /locus_tag="ACHJWA_004601"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFO4311050.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGIVEIPVQMQIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPMGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(1573..2304)
                     /gene="artI"
                     /locus_tag="ACHJWA_004602"
     CDS             complement(1573..2304)
                     /gene="artI"
                     /locus_tag="ACHJWA_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFO4311051.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITAEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(2322..3050)
                     /gene="artP"
                     /locus_tag="ACHJWA_004603"
     CDS             complement(2322..3050)
                     /gene="artP"
                     /locus_tag="ACHJWA_004603"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFO4311052.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(3268..3783)
                     /locus_tag="ACHJWA_004604"
     CDS             complement(3268..3783)
                     /locus_tag="ACHJWA_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO4311053.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYHIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            4407..>5633
                     /locus_tag="ACHJWA_004605"
     CDS             4407..>5633
                     /locus_tag="ACHJWA_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311054.1"
                     /translation="MEYRFSWVIFNMINNISDQASSFPCTQLNQSDNFLDSLREFFAI
                     LNSSRKGELSTWDTIYLHLILVINADSDLIKNDVLLAENIPTADYQFNTFFSNAFETD
                     VKKYLGKSEDNEVEIKTGNERISIGIRNRSNGKLERQKFLFPLDYENKLQHQLDKHFT
                     IESHPLLYRHTMGSKIANVIFEKLYSRIDFNKEQYISFIKDAFLHFYDYSRRYAINEN
                     IAKDAVTNNIALMSTFYDSDNTSGEVLNNDFTEESFETALDVEHTIVLGFADDNFETK
                     PVHYQDLLTRFSAFQDTVFNLFPEMHSSHYNDICSVSVDMTKGTQCMIHLMVNEEVFM
                     SLPVPVATMVREDASNLINLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNES
                     ILKKCCFENGSLGDVKI"
BASE COUNT         1573 a         1269 c         1307 g         1484 t
ORIGIN      
        1 tgaaataaaa aaagacggac aacttagtga gttgtccgtc tttattataa gaatttatgc
       61 actatgtagg ccggataagg cgtccacgcc gcatccggca caggcaccgt gctgatgtct
      121 gatgcgacgc tggcgcgtct tatcagacct acaaaacccc cggcgaatgt acgcagccac
      181 attaattcct tcgctcaaac gccagcgctt tgcgctcgat cagacgcatc atcagcgtca
      241 gcaggccgtt aacgaccaga taaataatcc ctgccgcgcc gaacaccatg acatcgtagg
      301 tgcgtccgta caacaactgg ctgtatccca tcacttccat cagcgtaatg gtgtaagcca
      361 gagaggtact tttgaacacc agcaccactt cgttggaata agaagagagc gagcgtttaa
      421 aggcatacgg cagcaggatc gccagcgtat cttttttgct cattcccagg gcgctacagg
      481 actgccactg accttccggg atcgcacgaa ttgcaccgta aaacagctgc gtggtatacg
      541 ccgcactgtt cagcgacaac gcaatcagcg cacataacca cggttctgac aacaaatgcc
      601 acagtgccgg atactcctgc aacgtcggga actgacccgg cccgtaataa atcaggaaga
      661 tctgcaccag cagcggtgta ccggtaaaca gcgtgatata accccgcacc agccacacca
      721 gcaccggcgt tttcagcgtc aggatgatgg taaaaatcaa cgccagaatc agtgccacaa
      781 tcagcgaggc aacggttagc gtcaggctgg tgtgcagccc tttcatcagt tcgggtaaat
      841 actcaaacat tagctgggcc tccgctcaaa acgtgtcgcg cgcaggtcaa tgcgtttgag
      901 aatgtactga ctgagcaggg tgatcaccag gtaaatcgcc gccgccacaa tgtaccaggt
      961 aaatggttcc tgggtacgag tagcgatgct tttggtttgc agcattaaat cattcacact
     1021 aatcaaactg accagcgcgg tatctttcag cagcaccagc cactggttac cgaggccagg
     1081 cagcgcatga cgccacatct gcggcatcac cagacggaaa aagatagccg atttcgacag
     1141 ccccagcgcc tggccggatt cccactgacc catcggcacc gctttcaacg cgccgcgcag
     1201 cgtttgcgag gcataggcgg catacagcag tgacagagcg atgacaccac aaaggaacgg
     1261 gctaacgtcg aagttttcaa tctgcatctg caccgggatc tctacaatcc caagattgat
     1321 agtgaagcca tccgaaagtg tcagcagcag ctgcgaggag ccaaaataga taaacagcac
     1381 caccagaatt tctggcaggc cacgcagaat ggttaccagc gctgaacctg cccacgcgac
     1441 aggacgccat ttcgcagact cccataccgc aaagaacatc gccagcgcca gcccgacaat
     1501 caatgcacaa acggcaaggc cgacggtcat cccggcggcg cttgctaaag gaaaaaattc
     1561 attcatcagg aattacttct ggaaccattt gttgtagatg gtttcgtaag tgccatcttt
     1621 cttcactttt tccagcgcag tgttgagttt ctgctgcagc tcagtgttgc cctgacgtac
     1681 cgcgatgccg aggccagtac cgaagtaatc tttatcggtc actttatcgc ccactgccgc
     1741 cagtttcggg ttatctttca gccattcagt taccactgcg gtgtcaccaa atacgctgtc
     1801 gatacgaccg ttttgcagat ccagttttgc gttctggtag ctgtcatacg gcacggtggt
     1861 gatttccggg tgcttatcca taatgaattt ctggtgagtc gtaccattct gtacgccgac
     1921 ttttttgcct ttcagctgat caacgctggt gtatttaccc tgctgaccca caaacagggc
     1981 agagttgtca tagtacggcg tggtaaacag tacctgcttt tcacgttccg cggtgatatc
     2041 catcccggcc atcaccgctt ccacgcgacg gaatttcagg cttgggatca ggctgtcaaa
     2101 tgcctggtta gagaaagtac aggtcgcatc aatctcttta cacagcgcct gtgccaggtc
     2161 gacgtcaaaa ccaacgatct ggttgtttgc atcaatcgat tcaaacggag gataggaggc
     2221 ttcggtagca aaacgaatgg tttcggcagc tgtggcggaa agactaaaac ctgcaattaa
     2281 cgcggcaatc agaacttttt tcattgttgt tatcccgaat cttagtgaga gagatagttt
     2341 ttaaatgctt cggtttgcgg ctcggtaaag cagctcgcgt cgccttgttc tacgatatga
     2401 ccgttttcca tatacaccac tcggctggcg gttttacgcg ccacttcaac ttcgtgggtg
     2461 acgatcacct gggtaatatt cgtttctgcc agctcacgaa tgatgctgac gatttgtgcc
     2521 gtaatttccg ggtccagtgc ggcggtcggt tcatcaaaca gcagtacctg cggttccatc
     2581 attaacgcac gggcaatagc aacacgctgc tgctgaccac cagaaagatg cagcgggtaa
     2641 cgatcgctat aaggtttgag acgcagacgt tccagcagtt tttctgcacg ggccagcgcc
     2701 tgatctttac tcaaccccag tacacggcag ggcgcttcaa tcaggttttg ctgcacggtc
     2761 agatgcggcc acaggttgta ttgctgaaac accatgccaa cgttacgacg caaatcgcga
     2821 atcgctttgt ctgagggtgt tttggtgaaa tcgaaatggt tgcctgcaat gttgagcgta
     2881 ccggagcgcg gcatctcaag cagattgagt acacgcagca gcgagctttt acccgcgccg
     2941 ctggggccaa gtaacaccag cgtttcgccc tgtgggcaat ccagcgtgat atcgaacagc
     3001 gcctgatgcg cgccgtagaa gcaattaatg ccgtttaatt gaatactcat tgacactcgt
     3061 atactggcag tctgatagct attgaggtcg aagatagtac ctttgacaga ataattatgc
     3121 aatatttctg ctttaaaagt taaaagcaaa gcgcattatt caataaacat agcacaaaat
     3181 aacgggggcg gtggtcggcg agcataaatg tcggcattcc tcacgaaatg ccggacaatt
     3241 tacggggttt attggttgat caaggcgtta gcgattctcg atggactgac ggagcgtacc
     3301 cgccgtggca tgaacgctac cgcctaagta gcgcacatcg tcgatcaccc aacactggcc
     3361 ttcctgaatc attaacactt catcctgcca gccctggtca ccctgcttga gatccacgcg
     3421 caatggaatg ttacgggcat cacgattagg gatagtcgat gcactggcaa cgtgggcgct
     3481 gtctggcaag gtggttcgac tggagaacgg atcactgctt aacaattcac gatgactatt
     3541 atcgcggctg gcatcgctaa gtagggttgc cagtttgtca ctcaaatagg ggcgcagggc
     3601 ggtaatgtca ttgctgcgat gttgaatgtg gtagtcatag aattgttggg ccacgctgtc
     3661 aggccccccc tggacacagg aaccggtgcg gggaccatta tctttataag ctggcgtgac
     3721 ggtggtacag gcactcagca gcagtgcaca tggaataagt agagatagtg aacggtagcg
     3781 cataatcatt tccttattag cttgctgtct gcgtcacact acgaccaaac gctatcagat
     3841 tatttaatac attgttttta atacaattct gaaacaatct gaacacctga ttgggtttga
     3901 gtccattgct ggcgacccac tcaaggttgc tcgtagctga cttacttaaa ttacccaaac
     3961 aatagacagt gtaaaccatt tgtgaggttg agcgtgttat atggctgaaa tgatcattta
     4021 aggtaaatat gatagttctg aaatggtggt ataaatcctc ctgtagaggt taagagcatc
     4081 tgcaagaagc cagtttcaca tttataacgc gtgataaatg aacgcggtta ttgtctaatc
     4141 attacaaatt tttcacatgc gataagagat cctgctatta agatgattgc caaaattacg
     4201 ttgtattttt gaaaatgagc gaaatttgat agtcccagct ttgacttttg ataatattat
     4261 ttatgctaca gttgtgggta taaaatataa tctttttgaa caagtgaatt tgagctaaga
     4321 ataaatcaag ttgtttcagt gtttcgttaa ttaatatgag tataaaaggt gttgtgcttt
     4381 tataataata aagcgcgagg agttcggtgg agtatcgttt ttcttgggtg atttttaata
     4441 tgataaacaa tatatctgat caagcgtcat catttccatg cacacaattg aatcaaagtg
     4501 ataatttcct cgattcactg agggaatttt tcgcgatatt aaattccagt cgtaagggtg
     4561 agttgtctac atgggatacc atctacttgc atctgatatt agtaataaat gctgacagtg
     4621 atctcataaa aaatgatgtg ttgctcgcgg aaaatattcc aacagcggat tatcaattta
     4681 atacattttt tagtaatgct ttcgagactg atgttaaaaa atatctgggt aaatcagaag
     4741 ataacgaagt tgaaataaaa acgggtaatg aaagaatctc tattggaata cgtaacagat
     4801 cgaatggaaa acttgaaaga caaaagtttc ttttcccact ggactatgag aataaattac
     4861 aacatcaatt agacaagcat tttacgattg aatcgcaccc attattatac aggcatacta
     4921 tgggtagtaa gattgcgaat gtgatttttg aaaaattata cagtcgtatt gactttaaca
     4981 aagaacaata tatatccttt ataaaggacg cgtttcttca tttttatgat tatagcaggc
     5041 gatatgcaat taatgaaaat attgcaaaag acgctgtaac aaacaatatc gcattgatga
     5101 gcacttttta tgacagtgat aatacgtcag gtgaggtttt aaataatgat tttacagaag
     5161 agtcatttga aactgcgtta gatgtagagc atacaatagt gcttggattt gctgatgata
     5221 attttgaaac aaaacctgtt cattatcagg atttattaac acgatttagt gcctttcaag
     5281 ataccgtgtt taatctgttt cctgaaatgc actcttcgca ttacaacgat atttgtagcg
     5341 taagtgtgga tatgacaaag ggaacgcaat gcatgattca cttgatggtg aatgaagaag
     5401 ttttcatgtc tctgcctgtt ccagttgcca cgatggtacg tgaagatgca agtaatttaa
     5461 tcaatcttaa aacactactc aatgatggtt gctttattaa atatagtcat tttaatgacg
     5521 tggcattaat aaagcaaaac ataagtaatc tctatttatc acacactgtt gtgaatgaaa
     5581 gcatattgaa aaaatgttgt tttgaaaacg gcagtttagg tgatgttaaa ata
//
LOCUS       DAZFAB010000189         5610 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZFAB010000189 DAZFAB010000000
VERSION     DAZFAB010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5610
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.189"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..396
                     /gene="cesT"
                     /locus_tag="ACHJWA_004606"
     CDS             <1..396
                     /gene="cesT"
                     /locus_tag="ACHJWA_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesT"
                     /protein_id="HFO4311055.1"
                     /translation="NRLCSFAIDEIYYISLSDANDEYMMIYGVCGKFPTDNSNFALEI
                     LNANLWFAENGGPYLCYEAGAQSLLLALRFPLDDATPEKLENEIEVVVKSMENLYLVL
                     HNQGITLENEHMKIEEISSSDNKHYYAGR"
     gene            456..3260
                     /gene="eae"
                     /locus_tag="ACHJWA_004607"
     CDS             456..3260
                     /gene="eae"
                     /locus_tag="ACHJWA_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin type gamma"
                     /protein_id="HFO4311056.1"
                     /translation="MITHGCYTRTRHKHKLKKTLIMLSAGLGLFFYVNQNSFANGENY
                     FKLGSDSKLLTHDSYQNRLFYTLKTGETVADLSKSQDINLSTIWSLNKHLYSSESEMM
                     KAAPGQQIILPLKKLPFEYSALPLLGSAPLVAAGGVAGHTNKLTKMSPDVTKSNMTDD
                     KALNYAAQQAASLGSQLQSRSLNGDYAKDTALGIAGNQASSQLQAWLQHYGTAEVNLQ
                     SGNNFDGSSLDFLLPFYDSEKMLAFGQVGARYIDSRFTANLGAGQRFFLPANMLGYNV
                     FIDQDFSGDNTRLGIGGEYWRDYFKSSVNGYFRMSGWHESYNKKDYDERPANGFDIRF
                     NGYLPSYPALGAKLIYEQYYGDNVALFNSDKLQSNPGAATVGVNYTPIPLVTMGIDYR
                     HGTGNENDLLYSMQFRYQFDKSWSQQIEPQYVNELRTLSGSRYDLVQRNNNIILEYKK
                     QDILSLNIPHDINGTEHSTQKIQLIVKSKYGLDRIVWDDSALRSQGGQIQHSGSQSAQ
                     DYQAILPAYVQGGSNIYKVTARAYDRNGNSSNNVQLTITVLSNGQVVDQVGVTDFTAD
                     KTSAKADNADTITYTATVKKNGVAQANVPVSFNIVSGTATLGANSAKTDANGKATVTL
                     KSSTPGQVVVSAKTAEMTSALNASAVIFFDQTKASITEIKADKTTAVANGKDAIKYTV
                     KVMKNGQPVNNQSVTFSTNFGMFNGKSQTQATTGNDGRATITLTSSSAGKATVSATVS
                     DGAEVKATEVTFFDELKIDNKVDIIGNNVRGELPNIWLQYGQFKLKASGGDGTYSWYS
                     ENTSIATVDASGKVTLNGKGSVVIKATSGDKQTVSYTIKAPSYMIKVDKQAYYADAMS
                     ICKNLLPSTQTVLSDIYDSWGAANKYSHYSSMNSITAWIKQTSSEQRSGVSSTYNLIT
                     QNPLPGVNVNTPNVYAVCVE"
     gene            complement(3524..4744)
                     /gene="escD"
                     /locus_tag="ACHJWA_004608"
     CDS             complement(3524..4744)
                     /gene="escD"
                     /locus_tag="ACHJWA_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscD"
                     /protein_id="HFO4311057.1"
                     /translation="MLSSYKIKLLNGAMRNRELQLPMGNLTLGTEDNDIVYFPLEQGL
                     NQFLLDIREEGVFLLSPVEFWIDGQPTPYEADKPLPVGKVIDIAGCCFIIGDIDHSLP
                     LSDVPERFSAKSRRKKRLILASVIGATFALSGAIGSYVLLSPKAEPPTFTRADVYQQL
                     KENKLHAITLVWHGKNIALYGRCESTTDLTPFFNYLKEKNIFYYNNIICNNQIISAIN
                     DVLTEYGYKDIIITKGNKPGFFLLSGYIPPSPKWSEVENLLLNTPGVAGWEIHNNSNN
                     KINELASEFKKNKLINYVNIFKKNDVIIVAGEVSQQNESKILAIINAMNKNSNAKILF
                     QNIQPYISADIFPGKILRISGTMKNPTIALDNGTSLGIGSILKGGYVIDAIDPKDGIN
                     ISRPDEYIHIPLSY"
     gene            4887..>5610
                     /gene="sepL"
                     /locus_tag="ACHJWA_004609"
     CDS             4887..>5610
                     /gene="sepL"
                     /locus_tag="ACHJWA_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE gatekeeper SepL"
                     /protein_id="HFO4311058.1"
                     /translation="MANGIEFNQNPASVFNSNSLDFELESQQLTQKNSSNISSPLINL
                     QNELAMITSSSLSETIEGLSLGYRKGSARKEEEGSTIEKLLNDMQELLTLTDSDKIKE
                     LSLKNSGLLEQHDPTLAMFGNMPKGEIVALISSLLQSKFVKIELKKKYARLLLDLLGE
                     DDWELALLSWLGVGELNQEGIQKIKKLYEKAKDEDSENGASLLDWFMEIKDLPEREKH
                     LKVIIRALSFDLSYMSSFEDKVK"
BASE COUNT         1689 a         1041 c         1210 g         1670 t
ORIGIN      
        1 aacagattgt gttcttttgc tattgatgaa atttattata tttcgttatc tgatgccaat
       61 gacgaatata tgatgattta tggtgtctgt gggaaattcc cgacagataa ctctaacttc
      121 gctcttgaga ttttgaatgc aaacttatgg tttgcagaga atggtgggcc atatctgtgc
      181 tatgaggctg gagcacaatc gctgttgtta gcgttacgtt tccctctcga tgatgctacc
      241 cctgaaaaac tcgagaatga aatagaagtc gttgttaagt caatggaaaa cctgtatttg
      301 gtattacata atcagggaat aacattagaa aacgaacata tgaaaataga ggaaatcagt
      361 tcaagcgaca ataaacatta ttacgccgga agataaaatc cgatctatta atataattta
      421 tttctcattc taactcattg tggtggagcc ataacatgat tactcatggt tgttataccc
      481 ggacccggca caagcataag ctaaaaaaaa cattgattat gcttagtgct ggtttaggat
      541 tgttttttta tgttaatcag aattcatttg caaatggtga aaattatttt aaattgggtt
      601 cggattcaaa actgttaact catgatagct atcagaatcg ccttttttat acgttgaaaa
      661 ctggtgaaac tgttgccgat ctttctaaat cgcaagatat taatttatcg acgatttggt
      721 cgttgaataa gcatttatac agttctgaaa gcgaaatgat gaaggccgcg cctggtcagc
      781 agatcatttt gccactcaaa aaacttccct ttgaatacag tgcactacca cttttaggtt
      841 cggcacctct tgttgctgca ggtggtgttg ctggtcacac gaataaactg actaaaatgt
      901 ccccggacgt gaccaaaagc aacatgaccg atgacaaggc attaaattat gcggcacaac
      961 aggcggcgag tctcggtagc cagcttcagt cgcgatctct gaacggcgat tacgcgaaag
     1021 ataccgctct tggtatcgct ggtaaccagg cttcgtcaca gttgcaggcc tggttacaac
     1081 attatggaac ggcagaggtt aatctgcaga gtggtaataa ctttgacggt agttcactgg
     1141 acttcttatt accgttctat gattccgaaa aaatgctggc atttggtcag gtcggagcgc
     1201 gttacattga ctcccgcttt acggcaaatt taggtgcggg tcagcgtttt ttccttcctg
     1261 caaacatgtt gggctataac gtcttcattg atcaggattt ttctggtgat aatacccgtt
     1321 taggtattgg tggcgaatac tggcgagact atttcaaaag tagcgttaac ggctatttcc
     1381 gcatgagcgg ctggcatgag tcatacaata agaaagacta tgatgagcgc ccagcaaatg
     1441 gcttcgatat ccgttttaat ggctatctac cgtcatatcc ggcattaggc gccaagctga
     1501 tatatgagca gtattatggt gataatgttg ctttgtttaa ttctgataag ctgcagtcga
     1561 atcctggtgc ggcgaccgtt ggtgtaaact atactccgat tcctctggtg acgatgggga
     1621 tcgattaccg tcatggtacg ggtaatgaaa atgatctcct ttactcaatg cagttccgtt
     1681 atcagtttga taaatcgtgg tctcagcaaa ttgaaccaca gtatgttaac gagttaagaa
     1741 cattatcagg cagccgttac gatctggttc agcgtaataa caatattatt ctggagtaca
     1801 agaagcagga tattctttct ctgaatattc cgcatgatat taatggtact gaacacagta
     1861 cgcagaagat tcagttgatc gttaagagca aatacggtct ggatcgtatc gtctgggatg
     1921 atagtgcatt acgcagtcag ggcggtcaga ttcagcatag cggaagccaa agcgcacaag
     1981 actaccaggc tattttgcct gcttatgtgc aaggtggcag caatatttat aaagtgacgg
     2041 ctcgcgccta tgaccgtaat ggcaatagct ctaacaatgt acagcttact attaccgttc
     2101 tgtcgaatgg tcaagttgtc gaccaggttg gggtaacgga ctttacggcg gataagactt
     2161 cggctaaagc ggataacgcc gataccatta cttataccgc gacggtgaaa aagaatgggg
     2221 tagctcaggc taatgtccct gtttcattta atattgtttc aggaactgca actcttgggg
     2281 caaatagtgc caaaacggat gctaacggta aggcaaccgt aacgttgaag tcgagtacgc
     2341 caggacaggt cgtcgtgtct gctaaaaccg cggagatgac ttcagcactt aatgccagtg
     2401 cggttatatt ttttgatcaa accaaggcca gcattactga gattaaggct gataagacaa
     2461 ctgcagtagc aaatggtaag gatgctatta aatatactgt aaaagttatg aaaaacggtc
     2521 agccagttaa taatcaatcc gttacattct caacaaactt tgggatgttc aacggtaagt
     2581 ctcaaacgca agcaaccacg ggaaatgatg gtcgtgcgac gataacacta acttccagtt
     2641 ccgccggtaa agcgactgtt agtgcgacag tcagtgatgg ggctgaggtt aaagcgactg
     2701 aggtcacttt ttttgatgaa ctgaaaattg acaacaaggt tgatattatt ggtaacaatg
     2761 tcagaggcga gttgcctaat atttggctgc aatatggtca gtttaaactg aaagcaagcg
     2821 gtggtgatgg tacatattca tggtattcag aaaataccag tatcgcgact gtcgatgcat
     2881 cagggaaagt cactttgaat ggtaaaggca gtgtcgtaat taaagccaca tctggtgata
     2941 agcaaacagt aagttacact ataaaagcac cgtcgtatat gataaaagtg gataagcaag
     3001 cctattatgc tgatgctatg tccatttgca aaaatttatt accatccaca cagacggtat
     3061 tgtcagatat ttatgactca tggggggctg caaataaata tagccattat agttctatga
     3121 actcaataac tgcttggatt aaacagacat ctagtgagca gcgttctgga gtatcaagca
     3181 cttataacct aataacacaa aaccctcttc ctggggttaa tgttaatact ccaaatgtct
     3241 atgcggtttg tgtagaataa ttccataacc accccggcta aaatatgtat tgttttagtc
     3301 ggggcataat tatttcttct taagaaataa cctcttataa tcaaatctac tactggtctt
     3361 tttatctgct taataggtct ctttcaaaga gacacattca cgttttctca tgtaggttga
     3421 tccaaccacg ctgataccaa agctgaatca catcaagcaa caactatgct cacaacatcc
     3481 acacaataaa aaaccctccg aagaggggga agaggataag aaattaatac gacagtggaa
     3541 tatgtatata ttcgtctggg cgggagatgt tgataccatc tttaggatca atcgcgtcta
     3601 tgacatagcc tcctttcaga atgctaccaa tccccaggct ggtgccatta tcaagagcaa
     3661 ttgttggatt cttcatcgta ccgctgattc tgagtatttt tccggggaaa atatctgctg
     3721 aaatgtacgg ctgaatgttc tgaaagagta tcttggcgtt actatttttg ttcattgcat
     3781 ttataatggc aaggatctta ctctcattct gttgagaaac ttcccccgca acaatgatga
     3841 catcattctt tttgaaaata ttgacatagt ttattagttt attcttttta aactctgatg
     3901 ccagttcatt gattttattg ttactgttgt tgtgtatttc ccatcctgca actcctggcg
     3961 tattcagtaa cagattttca acctcgctcc atttgggtga agggggaatg tatcccgata
     4021 acagaaagaa tccaggctta ttaccttttg taataatgat atctttgtaa ccgtattccg
     4081 ttaagacatc attaattgcg gatatgattt ggttattgca aatgatatta ttgtaataaa
     4141 aaatattttt ttcttttagg taattaaaaa atggagttaa atccgtggtt gattcgcaac
     4201 gcccataaag cgcgatattt ttcccatgcc ataccagagt gattgcatga agtttgtttt
     4261 cttttagctg ctgatataca tcagctctgg taaacgttgg gggttcagct tttggggaaa
     4321 gcaaaacgta actgccgatg gcaccagaca acgcaaaagt agcaccaatc acgctcgcca
     4381 ggataaggcg ttttttgcgt cgacttttgg cagagaacct ttctggcaca tcagacaatg
     4441 gcaaggaatg gtcaatatca ccaatgatga agcaacatcc cgctatatca ataacctttc
     4501 cgacaggtaa tggtttatct gcttcatagg gcgtgggttg cccatcaatc caaaattcaa
     4561 ccggagataa aaggaaaaca ccttcctcac gtatatcaag taaaaactga ttgaggcctt
     4621 gttcaagggg aaagtaaaca atatcgttat cttccgtacc tagcgttagg tttcccatcg
     4681 gaagttgtaa ttcccgatta cgcatcgcac cattgagaag ttttatttta tatgaggata
     4741 acatttttaa attttattca tcctggtggt tgaacaatga gaaaaaatat ggtgaactta
     4801 catcgtctaa gaatagagta gaaaggaagc tgttgtacag tactatttaa tggaatattc
     4861 ataattaatg attacgtgag tttccaatgg ctaatggtat tgaatttaat caaaaccccg
     4921 catctgtttt taattctaat tcattagatt ttgaattaga atctcagcaa ttaacgcaaa
     4981 aaaattcttc taatatttct tcgccattaa taaatttgca aaatgaatta gcaatgatta
     5041 ctagttcatc gctttctgaa acgattgaag ggctaagcct gggatatcgc aaaggtagcg
     5101 caaggaaaga agaggaaggc tcgacaatcg agaagctgct caatgatatg caagagctgc
     5161 ttactcttac cgatagtgat aaaataaaag aactatcatt aaaaaacagc gggctgctgg
     5221 aacaacacga tcctactttg gcgatgtttg gcaacatgcc aaagggggaa attgttgcgc
     5281 taatatcttc tttattgcaa tctaagtttg ttaagattga attaaaaaag aaatatgcca
     5341 ggttattatt agatttatta ggcgaagatg attgggagct ggctctgctt tcctggttag
     5401 gggtgggtga gttaaatcag gaaggtatcc agaagatcaa gaagctttat gaaaaggcta
     5461 aggatgagga ttctgaaaat ggcgcctctt tacttgactg gtttatggag attaaggatc
     5521 ttcccgagcg tgagaagcac ttaaaagtca ttattagggc gctgtcgttc gatctctctt
     5581 atatgtcttc ttttgaagac aaagtaaaga
//
LOCUS       DAZFAB010000190         5528 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZFAB010000190 DAZFAB010000000
VERSION     DAZFAB010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5528
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.190"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(113..451)
                     /locus_tag="ACHJWA_004610"
     CDS             complement(113..451)
                     /locus_tag="ACHJWA_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311059.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSGGEYDASHLRPERRRRDILLEEVLRQAGIPLLRSHDARKLLQMTGEWLNTTG
                     AAQQSPEHRS"
     gene            complement(489..698)
                     /gene="hha"
                     /locus_tag="ACHJWA_004611"
     CDS             complement(489..698)
                     /gene="hha"
                     /locus_tag="ACHJWA_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFO4311060.1"
                     /translation="MEKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSTSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            complement(744..1205)
                     /locus_tag="ACHJWA_004612"
     CDS             complement(744..1205)
                     /locus_tag="ACHJWA_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFO4311061.1"
                     /translation="MRKYIPLVLFIFSWPVLCADIHGRVVRVLDGDTIEVMDSRKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRMLGQVYAPDGMNVN
                     QFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            complement(1450..1662)
                     /locus_tag="ACHJWA_004613"
     CDS             complement(1450..1662)
                     /locus_tag="ACHJWA_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFO4311062.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGSRAVILEVSGRWMEAAEAWRRAAG
                     VAPRTDWQQFARKRAEHCHRRCRGRG"
     gene            complement(1795..2355)
                     /locus_tag="ACHJWA_004614"
     CDS             complement(1795..2355)
                     /locus_tag="ACHJWA_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFO4311063.1"
                     /translation="MAEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTQDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            complement(2458..3318)
                     /locus_tag="ACHJWA_004615"
     CDS             complement(2458..3318)
                     /locus_tag="ACHJWA_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO4311064.1"
                     /translation="MKITDHKLSEGIALTFRVPEGNIKHPLIILCHGFCGIRNVLLPC
                     FANAFTEAGFATITFDYRGFGESDGERGRLVPAMQTEDIISVINWAEKQECIDNQRIG
                     LWGTSLGGGHVFSAAAQDQRVKCIVSQLAFADGDVLVTGEMNESERASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLSDDESKVFFEKIKARHPEMDIKIPFLTVMETLQYKPAESAA
                     SVQCPVLVVIAGQDSVNPPEQGRALYDAVASGTKELYEEADACHYDIYEGAFFERVVA
                     VQTQWFKQYL"
     gene            complement(3377..4123)
                     /gene="traX"
                     /locus_tag="ACHJWA_004616"
     CDS             complement(3377..4123)
                     /gene="traX"
                     /locus_tag="ACHJWA_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HFO4311065.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSLLVWSPGQRDIIKTVALVLM
                     VLDHANRILHLDQTWMFLAGRGAFPLFALVWGLNLSRHAHIRQSAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSHRLYRAEDRTERLVLVACLLAVIPALNLASSDAAAVAGLVMTVLTVGLVSC
                     AGKSLPRFWPGDFFPTFYGCHLAVLGILAL"
     gene            complement(4143..>5528)
                     /locus_tag="ACHJWA_004617"
     CDS             complement(4143..>5528)
                     /locus_tag="ACHJWA_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI
                     domain-containing protein"
                     /protein_id="HFO4311066.1"
                     /translation="RIAGISRDDGLITLQDAEGNTRLISPREAVAEGVTLYPPDTIRV
                     GTGDRMRFTKSDRGRGYVANSVWTVTAVSGDSVTLSGGQQTRVIRPAQERAEQHIDLA
                     YAITAHGAQGASETFAIALEGTEGSRKLMAGFESAYVALSRMKQHVQVYTDNRQGWTD
                     AINNAVQKGTAHDVFEPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPA
                     RFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGEGRVKGSGDAQF
                     VALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAITGGRVWGDIP
                     DNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLPDGRTEQA
                     VREIAGQERERAVTSEREAALPESVLREPQREREAVREVVRENLLQERLQQMERDMVR
                     DLQKEKTLGGD"
BASE COUNT         1232 a         1651 c         1237 g         1408 t
ORIGIN      
        1 aatgtgattt aaactgtgaa atagtatggt tttcagttat ggaaacgccg ggagcgggga
       61 aaacttgctt tttcccgttt ccggggttgg acaactgagc aacgcgaagg cgtcagctac
      121 gatgttccgg tgactgctga gcagcccctg ttgtattcag ccattctccg gtcatctgca
      181 gcagttttct ggcatcgtgg cttctgagca acggaatacc agcctgcctc agaacctctt
      241 ccagaagaat atcccggcgt ctgcgttcag gtcgtaaatg gctggcatca tattcaccgc
      301 cggacagtat tcgctctgca tcaccggtcc gtatacctgc cgcatgcaat tgcatcagca
      361 aaggagaatg ctgatttaca cttctgagga taatccggat aagtaacgct gcaataatca
      421 gaacaatgag aataaaaata atcagcttca ttgtttatct gctcctggta attaattatg
      481 ctgaactatt accttacata tttccatatt tcttttggta tcctgtcata taactttccg
      541 gtgattaatt ctgctaaccg gtggtctgct gctgaattaa atgattcacg ttcactggtc
      601 gataaactgt ctctgttttt atgaataatt ttttccagcg tattgactga tgaacatcgc
      661 cgtaattgat acagccattc ctgttttgtt ttttccattt ttattcccgc tgagaacaga
      721 tgaatgataa gattgccatc gctttattta cggtgtctcc atatccaggg gggaaccgga
      781 tcagcatctg accagagccc acgtttttgt tgtctggctt cgttctgaag gacaggcagc
      841 acgggatctg tgttgtactg ctcatatacc caggcggccc cggcccggac cataaactga
      901 ttaacattca ttccgtctgg tgcataaacc tgcccgagca tacgaccata acgatctcgc
      961 tgaaaatatg tgacggtaac cgtttttcct gcaaccagtg atttcatcat gtctgtcgac
     1021 caccggccat aatcctgttt cttttccggc gcatcaatat ttaccagcct gatccgcacc
     1081 gccttccgtg agtccatgac ctcgatggta tcaccgtcca gtacacgaac aacccgtcca
     1141 tgaatatctg cacacaatac cggccatgaa aagataaata ataccagtgg aatatatttc
     1201 ctcatgttat tctccgtatc agataaatag cacctgtatt tcaggcatcc tgaatattgc
     1261 agcacacact taataacacc ttattttgtg aaattaatca caaaatataa aatcacgact
     1321 taatacggaa aaaactctgt gctactcaga actggccggt catactgccc gaccggatat
     1381 taaaaaaagc aaatctcatg cattattcag ccttctgaat ccctggagta atgaaggctg
     1441 cttttttcgt tacccccttc ctcggcagcg ccggtggcag tgttcagccc tttttcgggc
     1501 aaactgctgc cagtcagtcc ggggtgcaac accggcagcc cgtcgccatg cttctgccgc
     1561 ttccatccag cgtccactga cttccagaat aacggcccgg cttccggcac gctggtaacg
     1621 ccagacctga ccgttccgtg aactgtttct gaatccattc atctttatca ctcctcttta
     1681 acccgtcagt attatggtgt taaataatct actgaattgg cttcctttcg ggtacggaag
     1741 ttatttcctt ttaagcctta tttccctgcc tcctctctct gagggaggct ttttttattt
     1801 ctcatcaagc acggcctgaa gttctgcctt tatccggttc tggcggcgga ttttatccag
     1861 acgcgctgcc gcatacgctt cctcctcctg agaaatatgc tccgtcacat acccttccgt
     1921 gtcataccgg caggcaccgg ctttcatggc acacagatag ctttctgaac gggtgatggc
     1981 cttcagcgcc ctgcgcagct ttttatgcga gagcgggata ttccgctgcg ccacgtcttc
     2041 cagtaacacg tcccggatac cgcaggccag aagccggggc gtgtcaccgt caaataatcc
     2101 cggccaccag ggtttcaggg tgttcacggc ctcatcctgc gtgggcaggt tcagataaat
     2161 ggacagcgcc tgtctggcct gcgctttttt tgccgccagc tccgcttccc gggctgcttt
     2221 ctcggccagc ttctgctttt tcaccttcca ttttggtggc gtggtgacat tgatgatggt
     2281 tttccggctg cggacaggcg tctctccttc cgttttccgc ttcagtgtca gtaccggtcg
     2341 tttttgctct gccatgctta ctctgacctt cgtgaaaata tgccctgcag gccgttgaaa
     2401 cggctcatcg taacaggaag ttaatttcaa cgacctttaa cgtataaata ctgacaatta
     2461 aagatattgt ttaaaccatt gtgtctgcac agctactaca cgttcaaaaa acgcgccctc
     2521 ataaatatca taatggcatg catcagcttc ctcgtataat tccttagtac cagaagcaac
     2581 agcatcatat aatgctcttc cctgttccgg tggattaacg ctatcctggc cggcaataac
     2641 aaccaaaact gggcactgga cgctggctgc actctctgcc ggtttatatt gcaacgtttc
     2701 catgaccgta aggaaaggta tttttatatc catttcggga tgccgggctt tgattttctc
     2761 gaagaatacc ttcgattcat catcacttag tacccgtgta acccccacaa acatctcttt
     2821 cccggtgttt ttcttcttct ctgccatttt attcagcgtg gatagaaaag acgctctctc
     2881 cgattcattc atctctccgg taaccagaac atcaccgtca gcaaaagcca gctggctgac
     2941 aatacatttt acgcgttggt cctgcgcagc tgcactgaag acatgccccc ctcccagcga
     3001 tgttccccat aacccaatac gctgattatc aatacattcc tgcttttctg cccaattgat
     3061 aactgaaata atatcttcgg tctgcattgc aggaaccaga cgccctcgtt ctccatcact
     3121 ctcaccgaat cctcgataat caaaagtaat cgttgcgaat cctgcttctg tgaaggcatt
     3181 cgcaaagcaa gggagcagaa cattcctgat accgcaaaac ccatggcata agataatcaa
     3241 aggatgtttt atatttcctt caggaacacg aaatgtcagg gcaatacctt cagagagttt
     3301 gtgatccgta attttcataa atatcctgtg ttatgttgtt tattaataag cagaatcagc
     3361 gtcagtatca taccagtcac aacgccagaa tgcccagcac agccagatga cagccataga
     3421 acgtcgggaa aaaatctccg ggccagaaac ggggtaatga tttccctgca caggacacca
     3481 gaccaacggt cagcaccgtc atcacaagac cagctacagc cgccgcatca ctggaggcaa
     3541 ggttaagtgc cggaatcacg gccagcaggc atgcaaccag caccagacgt tccgttctgt
     3601 cttccgcccg gtacagccgg tggctgactg ccagcatcag cagcccggca atgccgtaac
     3661 tggtgccgga caaaggcccc cacagtgcca tcaggagaat ggccgctgct gtacgccacc
     3721 cactgcgcgt ttcacaccac gtcagcacct gtgccgccac tgcaaaggca aacaggatat
     3781 tcccctcata ccacggaaag cctgccagat aatacgcgaa ctgggcaata attccccatc
     3841 cccacagccg gttaatggcc gactgcctga tatgcgcatg gcgggacaga ttcagccccc
     3901 acaccagagc aaacaacgga aaggctccac gcccggccag aaacatccac gtctggtcca
     3961 gatgcagaat gcggtttgcg tggtccagta ccatcagcac cagtgccacc gttttgatga
     4021 tatcccgctg tccgggtgac cagaccagca acgactgcaa ccaggtatcg gtccgggcag
     4081 ccagtgaatc gttacgtgtc gtgttcgtgt tatccgttgt catcagtgtt tgtcttcctg
     4141 tatcagtctc cgcccagggt tttctctttc tgcaggtcac gaaccatatc ccgctccatc
     4201 tgctgcagtc gctcctgcag caggttttcc cggacaacct cacggaccgc ctcccgctcc
     4261 cgttgtggtt cacgcagtac actctccggc agcgcggctt cccgttcaga agtgacagcc
     4321 cgttcacgct cctgcccggc aatctccctg acagcctgct ctgttctgcc gtccggcagg
     4381 tcaggtttgt tctctgccat tttacggaca atttcatctg cgcggcgttc cgtttcacgg
     4441 cggatggcct cttcagcctg ccgctgtgcc agtacctctg ccgtgaccgg ctcgccattt
     4501 ccggctcccg gctggacgct gttgtccggg atatcccccc acacgcgacc accggtgatg
     4561 gcaccgggat tccacggacg accttcaccg gcgattctca ccaccacacc gctgtcagga
     4621 ttatcacggg caatccggac accatcctgc atattatcag ccagcaggct ctctccgtta
     4681 cggctgccct gcagggccac gaactgcgca tccccgctgc ctttcacccg tccttcacca
     4741 ctgaatcccc gcagcccgtt tccgtcatcc gtggtcagcg ggttcagcca gataccggca
     4801 gacttgccgt tacggtcaaa cgccggcagt gccacatacg gctgtggata tttacgtccc
     4861 ggagcaataa accgtgcagg actgtctccc ccggcaagcc ccgcctgacg aagaacggca
     4921 cgccctgccg ccacgtcccg cagctcccgc gccgtactga acagccgctc tgcattcatg
     4981 acctcccggt ccggtttcgg ttcaaacaca tcgtgggctg ttcctttctg tacggcattg
     5041 ttaatggcat ccgtccagcc ctgacggtta tcggtgtaca cctgcacatg ctgcttcata
     5101 cgcgacaggg ccacgtaggc tgactcaaag ccggccatca gtttccggct gccttccgta
     5161 ccttcaagcg cgatggcaaa ggtttcactt gccccctgcg caccgtgagc ggtgatggca
     5221 taggccaggt caatatgttg ctctgcccgc tcctgggcgg ggcgaatcac ccgggtctgc
     5281 tgtccgcccg acagcgtgac actgtcaccg gaaaccgctg tcaccgtcca gacgctgttg
     5341 gccacataac cacgcccccg gtcactcttc gtgaagcgca tccggtcacc ggtccccacc
     5401 ctgatggtgt ccggggggta cagtgtgacg ccttcagcca ccgcctcccg gggcgaaatc
     5461 agccgcgtgt taccttccgc atcctgcaga gttatcagcc cgtcatccct gctgataccg
     5521 gcaatccg
//
LOCUS       DAZFAB010000191         5080 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZFAB010000191 DAZFAB010000000
VERSION     DAZFAB010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5080
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.191"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            3..1061
                     /gene="tsuA"
                     /locus_tag="ACHJWA_004618"
     CDS             3..1061
                     /gene="tsuA"
                     /locus_tag="ACHJWA_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFO4311067.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGAFIAAKASREFRVRA
                     ADAQTTVRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWVGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            1075..1302
                     /gene="tsuB"
                     /locus_tag="ACHJWA_004619"
     CDS             1075..1302
                     /gene="tsuB"
                     /locus_tag="ACHJWA_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFO4311068.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(1345..2769)
                     /gene="sbcB"
                     /locus_tag="ACHJWA_004620"
     CDS             complement(1345..2769)
                     /gene="sbcB"
                     /locus_tag="ACHJWA_004620"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFO4311069.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSD
                     TLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYAEEIQMLA
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            2951..4129
                     /locus_tag="ACHJWA_004621"
     CDS             2951..4129
                     /locus_tag="ACHJWA_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4311070.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRPIF
                     DQIKPFLEKAMTTYNDVLFPGEDINKPISIAAANRFVNRIRGGMDLGYWRTHDFRRTL
                     VTRLSEMNVEPHVTERMLGHELGGIMSVYNKHDWIEAQRKAYELHADKLFWHIRSISD
                     "
     gene            complement(4110..4301)
                     /locus_tag="ACHJWA_004622"
     CDS             complement(4110..4301)
                     /locus_tag="ACHJWA_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001576843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4311071.1"
                     /translation="MTDTSLIPEKEVMNKLGVSSRQTIWNYTKRHGFPKPVRTHPKSY
                     LREAVEGWILNGGVNQKCS"
     gene            complement(4379..4723)
                     /locus_tag="ACHJWA_004623"
     CDS             complement(4379..4723)
                     /locus_tag="ACHJWA_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311072.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHGFGAPH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKIHGNKAP
                     ETYRRISELRKD"
BASE COUNT         1248 a         1203 c         1279 g         1350 t
ORIGIN      
        1 ttatgttttc aatgatatta agcgggctaa tttgtggtgc tctgctggga tttgtcatgc
       61 agcgtgggcg tttttgcctg actggtggtt ttcgcgatat gtatatcgcg aaaaataatc
      121 gcatgtttta cgccttgctg attgcaattt cggtacaaag cgtgggggtt tttgcgttaa
      181 ttcaggcggg cctactgact tacgaagccg gggcgttccc gtggcttggt actgttatag
      241 gtgggtatct cttcgggctg ggaattgttc tggcgggtgg atgtgccacc gggacctggt
      301 atcgcgcggg tgagggattg atcggcagtt ggatcgcgct tttcacttat atggtgatga
      361 gtgcggtgat gcgttctcca cacgccagtg gtttaaatca aaccttgcag cactacacta
      421 ctgaacataa ctctattgct gagactttta atttgtctgt gtggccgttg gttgccgttt
      481 tgctggtgat aacgctctgg gtggtgatga aagagttgaa gaagccaaaa ctcaaagtcg
      541 cgaccttacc gccgcgccga accgggatcg ctcatattct gtttgaaaaa cgctggcatc
      601 cctttgtcac agctgtactc atcggtttga ttgcgctttt agcctggccc ctgagtgaag
      661 caaccggacg tatgtttggg ttaggcatca cttccccaac ggccaatatt ctgcaatttc
      721 tggtcgcggg tgacgtgaaa tacattaact ggggcgtttt cctggtgtta gggatcttcg
      781 tgggggcatt tattgcagcc aaagcgagcc gtgagttccg cgttcgcgca gctgatgcac
      841 aaacaacagt acgtagcggg ctaggtggtg tactgatggg cttcggtgcc agtattgcag
      901 gtggttgctc tatcggtaat ggactggtta tgactgcaat gatgacctgg cagggctggg
      961 ttggccttgt atttatgatt ctcggagtct ggactgcgtc ctggcttgtg tatgttcgac
     1021 cgcagcgtaa ggcgcgactg gcaactgctg cggcaaatta agtaaggtgt aaagatggcg
     1081 attaaaaagc tggatgtggt gacgcaggtc tgtccgtttc cactcataga agcaaaagca
     1141 gcactggcag aaatggccag cggcgatgag ttggtaattg agtttgattg cacccaggca
     1201 actgaagcaa tcccacagtg ggcggcagaa gaagggcatg ccatcaccga ttatcagcag
     1261 attggcgatg cagcgtggag catcaccgtt caaaaagcct gactcaacat tgtcctccgc
     1321 cgtaccagcg gcggaggctt cacattagac aatctcttcc gcgtactgcc aaagcgcttt
     1381 taacagcgcc actttttctt tatcgtcggc atactgctgc gccagcatct gaatctcttc
     1441 tgcatagcct tgtaaaaact ctggcgtgaa gacctggcgg cggtgctcca gccagcgttg
     1501 ctgctcggca taatccagcg tccccgggaa gttgcgtgcc cgataattga acaacagctt
     1561 ttcaatccgt ttatcaacaa aagtgatatc cagtgccggt aaattacgcg gctcggtttc
     1621 aagcacaatt ttcattgctg cacgatcggc gtcactgaaa aagccattat aaagctgtgc
     1681 atccacgtta tctgaaggcg taaacggttc ggcttccgcg aatatcgcca ccactttttc
     1741 acgcacttgc ggattttcac gcagaatttt caggttatcg aggcaatgct gacgattaat
     1801 tcccagtcgg tcggcatctt ccgggcgtag cgtattcgcc tgggccagca ccggacattt
     1861 attgatatgt accagtttaa ccggaacggc ggcgttatcg ccaagatcgg tttttgcggt
     1921 atataaacgc tcgcgcaatg tgtcgctatc cagttccagt aatggcgaaa tatctcctgc
     1981 caaatccacc ataattacgg cattgcgatt ttcaggatgc cacgccagcg gtgccaccca
     2041 gctggtattg ccgcgccatg ccccaaacat tccggaaacg tgaaccaggg gtttcatctg
     2101 cggaacatca atcaacgcca tcagtttgtg tttattacga tgggtaaaga gataatcaaa
     2161 caggcgtggt tgacgcgttt ttaccagttt cgccatcgca atagtggcgt acacatcagc
     2221 catcgcatcg tgggcgttgc tatgttcaat accattcgct ttggttaaat gctcaaggcg
     2281 aaagctcggt agaccgtcat cattttcagg ccagtttatt ccttccgggc gcagggcata
     2341 gcaggcacgc ataacatcca gtaaatccca gcgcgagtta tcatgctgcc agctccaggc
     2401 gtaaggatcg tagaaattac gataaaaaac gttgcgtgtg acttcgtcgt cgaaacgcac
     2461 attgttgtag cccagaatac aggtcttcgg tacggtaaaa agcgagtgaa tacgggcggc
     2521 aaatgcggct tcgttttctc ctttcgcccg agcttcctgc ggggtaatac cggtaattaa
     2581 tactgctcca ggctggggta aatagtcatc cgcgggcttg cagtaaaaga cttcgggttc
     2641 gccgatgaca ttgaattcgt catcggtgcg aatggctgcg aactgtgcag ggcgatctaa
     2701 cgcggggtgc gtgccaaagg tttcgtaatc gtgaaagagg aaggtaggtt gcttatctgt
     2761 gtctgtcatt gtctagcatt gtccaatgta gttaattaac tgtctgttat aactcgaaaa
     2821 acaacccttt tttcacctga gtatgtctac gaacgtcaaa tatagttgtt tactgtgcgc
     2881 tgttgtccat gtgatattgt gtaccagatg tgtaccaaac cattatttct gagcgtacca
     2941 aatattattt atggctataa gcgacacaaa actgcgtact atttatggta aaccatattc
     3001 tggcccacaa gaagtggctg atgccgatgg cctcagcgta cgaatttcac cgaagggggt
     3061 catccagttc cagtaccgct atcgctggca tggcaagcct aatcgacttg ggcttggtcg
     3121 atacccatcc ctgtctttga aggatgccag acagatcact gctgacttgc gaaagctcta
     3181 tttctcagga acggatccac gcacttattt tgaagagaag gtggagaact ccatgacggt
     3241 cgcccagtgt ctcgactact ggttcgacaa ctacgtctct acaactctca gagaaaagac
     3301 ccaagcactt taccgatcaa cggttatgaa gcgcatgcat gacgccttcc ctaatcgtcc
     3361 ggcatcttct atcacggtta agcaatgggt tgacctgctt accgaagaag aaaaagataa
     3421 tccacgccga gcaaggcagg tgctaagtca actaagatca gcaattagtt ggtgcatgcg
     3481 gcgtcagttg atagatagtt gcgcaattat gagcatccaa ccaagggact tcggctcccg
     3541 cgctgaggta ggggatcggg tactgtcgta tcacgaactg gctaagatct ggcttgctat
     3601 tgaaagaagc cgtgcgtcta cgtcaaataa gctccttcat cagatgctta tgctgtgggg
     3661 ggcgaggctc tcagagctta ggctggcaac aaagacagaa tttgacctgc tggacaacgt
     3721 atggaccgta ccgaaagagc atagcaagat gggtaatgtt atccgccgtc caatcttcga
     3781 tcaaattaag ccttttctcg aaaaggccat gacaacgtac aatgatgttc ttttccctgg
     3841 agaagacata aacaaaccga tcagcatcgc tgcagctaac cgattcgtaa atagaataag
     3901 gggagggatg gatctaggtt actggcgaac acatgatttc agaagaacgc ttgttacacg
     3961 tctgtccgag atgaatgtcg agcctcatgt tactgagcga atgctcggtc atgaacttgg
     4021 cgggataatg tccgtataca ataaacacga ctggatagag gctcagcgca aagcgtatga
     4081 gcttcacgct gataaattgt tctggcacat caggagcatt tctgattaac gccaccgtta
     4141 agaatccacc cttcaacagc ttcacgaagg tatgatttgg ggtgggttct gactggcttc
     4201 ggaaatccgt gccgtttggt atagttccag attgtctgac gtgatgaaac accgagcttg
     4261 ttcatcactt ctttctcagg aatcaggctg gtatcggtca tcttaattct ccaggcaaaa
     4321 agaaaccgcc atatagcggc tctatcagat atgaacaggc ctcatcgagt gtgaggcgtt
     4381 agtccttgcg tagctcgctg attcttctgt aagtctctgg tgctttgttt ccgtgtatct
     4441 tcatttcaga cttcaacaga gcaacgaggg aatcccattc gttgaggatt cctttgaatg
     4501 ccggaacgcg ctttgcaacc ttgtcgaatg aatctctgat ttctggaatc tgctcaacaa
     4561 gtgcaacgca tcgtctgaaa tcggctgcgt catgtggagc accgaagcca tgaccataga
     4621 tattcttttt caggccacat gcgattgagg caagagttgc gctactgatg ccgacatcgc
     4681 cagttgattg ccatttcaaa accttcatag ccaaatctga catttcttgt ctccataaaa
     4741 caaaactcgc cgtagcgagt tcagataaaa gaaatccccg cgagtgcgag gattgttagt
     4801 tgcgctctgc tgctgcctta gccattacca tatccacaca atttcaccag acatgattct
     4861 cgcaatcact atcatcacca aagtgataat cacaacttta actggcggca tcattcacca
     4921 tcctgctgcg gtgctgctgg atatactttt ctcatccaat gcgtggcgtc atgaataacg
     4981 aactgatatt catcatcagt aaacatcgag ccatcccact gtagcccgtc aagaacacct
     5041 ctggatggag agaataccca gatatcgaaa tcgtatttct
//
LOCUS       DAZFAB010000192         5043 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZFAB010000192 DAZFAB010000000
VERSION     DAZFAB010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5043
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.192"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..384
                     /locus_tag="ACHJWA_004624"
     CDS             <1..384
                     /locus_tag="ACHJWA_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO4311073.1"
                     /translation="LAEFVALISESGANPFGLTVDAVMEEYRRWRNESWRYDGSDKYP
                     WPQPVLYHICLEMRSKGIERQMTEGELKRLVERQLTKWAKHVGNGLSVPPVRRQLAAP
                     KRPPGPTPIELLKQEYERRKAAGFV"
     gene            419..1117
                     /locus_tag="ACHJWA_004625"
     CDS             419..1117
                     /locus_tag="ACHJWA_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO4311074.1"
                     /translation="METVFDALKALKKASSHEIAARLEISRDDAVTELWKLKRRGEAD
                     NKGSMWWLTSEATEAAPKTTAEMLINAIEQHGPQSADELALMFGITSRRANSSLAMAV
                     SKGRLIRVNQDGKYRYCIPGDNLPAEPKAASVTETDGKAFPQPAGVALPVREAETQEE
                     IKTESVAVTVQSQSSFIRKHPDGLILPSLHVANRELRRAKGQVQKWERLCAALREINK
                     HRDVIQKITTGDNG"
     gene            1139..1363
                     /locus_tag="ACHJWA_004626"
     CDS             1139..1363
                     /locus_tag="ACHJWA_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311075.1"
                     /translation="MKIRYHDFGPVSHMLISSTVLETRKHNHILDMLRLADPYLVINT
                     SGIFFLRSTVSGKTSHVLRAYKTAVREEGE"
     gene            1360..1716
                     /locus_tag="ACHJWA_004627"
     CDS             1360..1716
                     /locus_tag="ACHJWA_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="eae-like protein"
                     /protein_id="HFO4311076.1"
                     /translation="MSEINYQELRVELGAAKKRIEELEANRVVLEVENERLKHAMAVA
                     LEHISVTDAGQAGVAAMIIYDAMYHSEKTDPYAFLAELRAQGVEMVREHPAIKLCSLT
                     HICDELVAELRKGGNK"
     gene            1749..1901
                     /locus_tag="ACHJWA_004628"
     CDS             1749..1901
                     /locus_tag="ACHJWA_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311077.1"
                     /translation="MAKKHHYFAEGEITSICGGWMYFGNEREPDTFESPDDCKKCRRK
                     LNKGGK"
     gene            1898..2209
                     /locus_tag="ACHJWA_004629"
     CDS             1898..2209
                     /locus_tag="ACHJWA_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311078.1"
                     /translation="MSVIKEMPVERNEYGCWTHPEYEKFCDGREYISTEEFNAWMEKN
                     NLQWAIRSMDEDDFNLDADGPDISAWEPERPEGEGWFIGSIHDTEDGPVCVWLRNKAE
                     A"
     gene            2336..2899
                     /locus_tag="ACHJWA_004630"
     CDS             2336..2899
                     /locus_tag="ACHJWA_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311079.1"
                     /translation="MTELTKEWLQNTITGIESSRDEIPFGLDEDQNNMLTALKIALAS
                     LASVSDERAAYELFMEKRFGESVDRRRAKNGDREYMAWDMALGWIIWCHRAAMLQAGN
                     FRENKGSSTNNFRIISETSTNSPAIPDEVLSAILKVARLRADFDASEVDRRGIGSCLD
                     EAEQELIVTINEYASQIAVEATQGENQ"
     gene            3009..3113
                     /locus_tag="ACHJWA_004631"
     CDS             3009..3113
                     /locus_tag="ACHJWA_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311080.1"
                     /translation="MSDDISLVMEGALAVIAVVGVYCLVVFLMERLGN"
     gene            3357..3512
                     /gene="hokD"
                     /locus_tag="ACHJWA_004632"
     CDS             3357..3512
                     /gene="hokD"
                     /locus_tag="ACHJWA_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO4311081.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            3680..3958
                     /locus_tag="ACHJWA_004633"
     CDS             3680..3958
                     /locus_tag="ACHJWA_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311082.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDWYQHDPCTEEQAEW
                     LIQCYRRRGYEVKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            3960..5009
                     /locus_tag="ACHJWA_004634"
     CDS             3960..5009
                     /locus_tag="ACHJWA_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO4311083.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIRHAMNGTQERE
                     LSLAELSWWAACNQVVDALPEAVARRSLGLPAEKIRSVYRESDIVPGEQTAISILKQR
                     TKNIALPLHVHQQQNPPQKKTVVSIAVDPESPESFMRRPKRCRWVNEKYTRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKAHDIFTLPLCREHHNELHADPLEFEKKYGSQIEL
                     IFRFLDHAFATGVLG"
BASE COUNT         1355 a         1132 c         1408 g         1148 t
ORIGIN      
        1 ctggctgagt ttgtggcgtt gatttcggag agcggggcaa atccatttgg cctgacggtg
       61 gatgccgtga tggaggagta ccgtcgctgg aggaatgaat cctggcggta cgacgggagt
      121 gataaatacc cgtggccaca gccggtgctg taccacattt gcctcgagat gcgttcaaag
      181 gggattgagc gccagatgac cgaaggggag ttaaaacggc ttgtggaaag gcagctgacg
      241 aaatgggcaa agcatgttgg taatggcctc agtgttccgc cagtccggcg acaactggca
      301 gcacccaaac gcccgccagg gccaacgcca attgaattgc tgaaacagga atatgagcgc
      361 cggaaggcgg ccggttttgt ctgagttgag aagtaatttt taccgggagg aaaatttaat
      421 ggaaaccgtt tttgacgcac tgaaagcact gaaaaaagcc tcgtcgcacg agattgcagc
      481 ccgtcttgaa atcagccgtg acgatgctgt taccgaactc tggaagctga agcgtcgtgg
      541 cgaagctgat aacaaggggt cgatgtggtg gctgacgagt gaggcaactg aagcggcccc
      601 aaaaaccact gcagagatgc tgattaacgc gattgaacag catggtcctc agtcggctga
      661 cgaactggcg ttaatgttcg ggattacctc ccgccgggcg aattcatcac tggcgatggc
      721 tgtcagcaaa ggtcgtctga ttcgcgtaaa tcaggacggt aaatatcgtt actgcatacc
      781 gggcgataat ttaccggcag agccgaaagc tgcatcggta acggaaaccg atggtaaagc
      841 ctttcctcag ccggcaggtg ttgcgttacc agtccgggaa gcggaaacac aggaagaaat
      901 taaaactgaa agtgtggcgg tcacagtgca gtcacagtcg tcgttcatca gaaagcatcc
      961 ggatggtctg attttaccat cgctgcatgt ggctaaccgc gagctgcgcc gggcaaaagg
     1021 tcaggttcag aagtgggagc gcttatgcgc cgcgctgcgg gaaattaata agcatcgtga
     1081 tgtcatacag aaaataacga caggagataa cgggtaattt ttacagcagg gtgatgatat
     1141 gaaaatcaga tatcatgatt tcggccccgt gtcacatatg cttatttcaa gtacggtgct
     1201 ggaaacaaga aaacataatc acatactgga tatgctgcgt cttgctgacc cgtatctggt
     1261 aataaacacc agtggaattt tctttctgag aagcactgtg tcaggaaaaa catcgcatgt
     1321 gcttcgggca tataaaacag cagtgcggga ggaaggagaa tgagcgagat taactatcag
     1381 gagctgcgtg ttgagctggg agcagcaaaa aaacgcatag aagagctgga ggctaatcgt
     1441 gtggtgcttg aggtggaaaa tgagaggttg aaacacgcaa tggccgtagc ccttgagcat
     1501 atatccgtca cagatgcagg acaggcaggt gtcgctgcaa tgattatcta tgatgccatg
     1561 taccacagtg aaaaaactga cccctacgct tttctggcag aattgcgggc gcagggtgtg
     1621 gagatggtgc gcgaacatcc agcaatcaaa ctttgctctt tgacgcacat atgtgacgag
     1681 ttagtcgcag agcttcgcaa aggaggcaac aagtgagtaa aacgaatccg ggctgggcaa
     1741 ggccacttat ggcgaaaaaa catcattatt ttgctgaagg tgaaataaca agtatttgcg
     1801 gtgggtggat gtattttggc aatgagcgtg agccggatac attcgaaagt ccagatgatt
     1861 gtaaaaagtg tcgtagaaaa ttaaataagg gggggaaatg agcgtaatta aagaaatgcc
     1921 agtggaacgt aatgaatatg gctgctggac gcatcctgaa tatgaaaaat tctgtgatgg
     1981 tcgggaatat atttcaacgg aagagtttaa cgcctggatg gagaaaaata atcttcagtg
     2041 ggctatccgt agtatggatg aagatgattt taatctggac gcagatggcc ctgatatttc
     2101 agcatgggaa cctgagcgac cagaaggtga gggatggttt atcggttcga tacatgacac
     2161 cgaagatggt cctgtttgtg tatggctgag aaataaggcc gaagcataaa ggctataaac
     2221 caacaactaa acactgaaaa tttaaatcag aaatgatttt tattaaatcc ttaaccggag
     2281 ggattcctgc accctcagaa catcaggaga ccgcccgaaa gggcggtagt gaaaaatgac
     2341 tgaattaacc aaagaatggc tacagaacac gattaccgga attgagtcat cacgggatga
     2401 aataccgttc ggactcgatg aagatcaaaa caacatgctt accgcattaa aaattgcact
     2461 ggcatcactg gcatcagtat cggatgaacg ggcagcctat gaattattta tggagaagcg
     2521 tttcggggaa tctgtagatc gccgcagagc aaaaaatggc gatagagaat acatggcatg
     2581 ggatatggcg cttggctgga ttatctggtg tcaccgcgcc gccatgcttc aggctggaaa
     2641 ctttcgggaa aataagggtt cgtcaaccaa taattttcgg ataatctcgg aaacgtcaac
     2701 caactctccg gcaatccctg atgaggtgtt gtccgcaatc ctgaaggtcg ccaggcttcg
     2761 tgcagatttc gatgcttctg aagtggacag gcgaggtatc ggtagttgtc tggatgaggc
     2821 cgagcaagaa cttatcgtta ccattaacga atacgccagt cagatcgcag tagaagcgac
     2881 acagggggag aaccaatgag ccggccagat gcatttgcag gcgttgctat agctattgct
     2941 tttctagtat atgttatttg tcggtggggg taaaaacgtt cgccgggatt cacaccaagg
     3001 gagggaatat gtcggatgat atttcactgg taatggaagg cgctctggct gttattgctg
     3061 ttgtgggtgt ttactgcctg gttgtgtttt tgatggagcg cctggggaac tgaattcatt
     3121 ccgtatggga attcccatat cgggcaaaac ggtttgctgt aaagcgagag ttaagtagaa
     3181 ttgctgcggg tgcttgaggc tgtctgcctc gggcatgcca ccgtaaggca gacagagaaa
     3241 agccccagtt aacattacgc gtcctgcaag acgcttaaca ttaatctgag gcccaatcta
     3301 tgcttcacaa acgtaggtta gcctcttacg cgccgaaagg caaggagaag caggttatga
     3361 agcagcaaaa ggcgatgtta atcgccctga tcgtcatctg tttaaccgtc atagtgacgg
     3421 cactggtaac gaggaaagac ctctgcgagg tacgaatccg aaccggccag acggaggtcg
     3481 ctgtcttcac agcttacgaa cctgaggagt aagagaccag gcgggggaga aatccctcgc
     3541 caccgctgat gtgtcaggca tcctcaacgc acccgcactt aacccgcttc ggcgggtttt
     3601 gttttttctg gtcgttctgg tttacaatcc atccgtcagc ctgaacaact ggcacctgct
     3661 gcgccagcag agaaaacaga tggcgcacga taccaaattt tacaattcgg ataactctgc
     3721 cgcccctgcc agcaggcacg ggcggcgttc tcatgcattc aaatctgact ggtatcagca
     3781 cgacccctgc accgaagaac aggctgaatg gctgattcag tgttaccgca ggcgcggata
     3841 cgaggttaag aaagccctca gtctcgatta tcgtcactgg ataatctccg tcaggcttcc
     3901 ttactccgaa cgcccaccgc gtccgtcccg cacattccag caacgcatct ggaggtaacg
     3961 tgcgggtatt acttcgacct gttctggtac cggaactcgg gctggtgatc gttaagccgg
     4021 gccgtgaatc catgccggta ttccacaata cccgggtact ggtggagccg gaaccgaaaa
     4081 gcatgcgtaa tctgccgtcc ggggtcgttc ctgccgttcg ccagccgctg gcggaggata
     4141 aatcattact gccatttttc agcgacgaac gagtgattcg tgctgctggt ggcgctggcg
     4201 cactgtctga ctggttactg cgccatgtta aatcctgcca gtggccacac ggcgattatc
     4261 accacagtga aaccgtcatt caccgttatg gtaccggcgc aatggtgttg tgctggcact
     4321 gcgacaacca gttgcgtgac cagacctccg aatcacttga gcaacttgct caccaaaacc
     4381 tgtcagcatg gatgattgac gtcattcgtc acgcaatgaa tggcacacag gagcgtgaat
     4441 tatcgctggc tgaattatcc tggtgggcgg cctgcaatca ggtggtggat gcactacctg
     4501 aggcagtagc gcgtcgttcg ctgggattac cagcggaaaa aatccgctcc gtataccgtg
     4561 agagtgacat cgtaccggga gaacagacag ccatcagcat actgaagcag cgcacaaaaa
     4621 atattgcgct gccacttcac gtccaccagc aacaaaatcc accacagaaa aaaacggttg
     4681 tcagtatcgc cgttgatccg gagtctcctg aatcgttcat gaggcggcct aaacgttgcc
     4741 gctgggttaa tgagaaatac acgcgctggg taaagacaca gccgtgtgcg tgttgtggta
     4801 agcctgctga cgatccgcat cacctgattg gtcatggtca ggggggaatg gggacaaagg
     4861 cccacgatat tttcacgcta ccgttgtgcc gggagcacca caacgaactt catgcagacc
     4921 cgctggagtt tgagaaaaag tacggctctc agattgagtt aatttttcgt tttcttgatc
     4981 acgcctttgc gactggcgtg ctcgggtaaa agaggtgact gatgctcata gatttggttt
     5041 tac
//
LOCUS       DAZFAB010000193         4965 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZFAB010000193 DAZFAB010000000
VERSION     DAZFAB010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4965
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.193"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..612)
                     /gene="yibB"
                     /locus_tag="ACHJWA_004635"
     CDS             complement(<1..612)
                     /gene="yibB"
                     /locus_tag="ACHJWA_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HFO4311084.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRVEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDESKMHLFTIKKGLTVTSQQQVFDFMIGNHVYIIGGAI"
     gene            916..1848
                     /gene="rfaD"
                     /locus_tag="ACHJWA_004636"
     CDS             916..1848
                     /gene="rfaD"
                     /locus_tag="ACHJWA_004636"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFO4311085.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LNIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            1858..2904
                     /gene="rfaF"
                     /locus_tag="ACHJWA_004637"
     CDS             1858..2904
                     /gene="rfaF"
                     /locus_tag="ACHJWA_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFO4311086.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLKELNALLLQEEA"
     gene            2908..3900
                     /gene="rfaC"
                     /locus_tag="ACHJWA_004638"
     CDS             2908..3900
                     /gene="rfaC"
                     /locus_tag="ACHJWA_004638"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFO4311087.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPEIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGENQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            3897..>4965
                     /gene="rfaL"
                     /locus_tag="ACHJWA_004639"
     CDS             3897..>4965
                     /gene="rfaL"
                     /locus_tag="ACHJWA_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HFO4311088.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEIFKAII
                     ENTLLCTIAIPIILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVAIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNK"
BASE COUNT         1352 a         1106 c         1153 g         1354 t
ORIGIN      
        1 aattgcgccc ccaataatat atacatgatt gccaatcatg aaatcaaaaa cctgttgttg
       61 agatgtgaca gtaagccctt tttttatagt gaaaagatgc atcttacttt catcaaatgg
      121 gaaatcccaa attttcaatc ctcgagttac atttggctta cggcaatagc caaaatctat
      181 ccacgcaaca agaggcgttt tcactaagcc catgttgata gctttattta caaaataggc
      241 cttaaggtta cataccaaaa catactctgg tgaccagtac tctgggtttt ttaattgtcg
      301 aggctcaagt ctatttgtaa acgactcatc tttttgaatt ttttcgatac gacttctgat
      361 atatctaaat ttctttttta tatcgataac gataaccgtt gttggttttc cgttgcgaat
      421 cgcttcaacc cttggtttca aatcgggaga agtgaaaata atcatctcat tttcaagtgc
      481 ggccagacgc tcaaagtaac taaaataaac atcaactgaa cgagctagtt tttcacggaa
      541 ccctttatta gcagtccaat cccctctacc aatgtcaaaa tatgcagtga taattgtggt
      601 ggatgatttc attactaata agaccattaa tataaaaaaa gaattatatc ataaatatta
      661 acagaatgta tactgtatat tatccattca aatgcttccg ttatttatca tacatcggaa
      721 tattgatact aaagcactat tcacatgcaa aaccaacatc cgccatgaag gactagctaa
      781 aacccaaact agtttgttgc aattagcatc cttgcacctc tatgtaaagg gctgaaggga
      841 ttcggatgtg atggtatgat tacagacatt cgtgtctgag attgtctctg actccataat
      901 tcgaaggtta cagttatgat catcgttacc ggcggcgcgg gctttatcgg cagcaacatc
      961 gttaaagccc tgaatgataa aggcatcacc gatattctgg tggtggacaa cctgaaagac
     1021 ggcaccaagt ttgtgaacct ggtggatctg aatatcgcag actatatgga taaggaagac
     1081 ttcctgatcc agattatggc tggcgaagag ttcggcgatg tcgaagcgat tttccacgaa
     1141 ggcgcgtgct cttccaccac cgagtgggac ggcaagtata tgatggataa caactatcaa
     1201 tactccaagg agctgctgca ctactgcctg gagcgtgaaa tcccgttcct gtacgcttct
     1261 tccgcagcca cctacggcgg acgcacctcc gactttattg aatcccgcga gtacgaaaaa
     1321 ccgttgaacg tctacggtta ctcaaaattc ctgtttgatg aatatgttcg ccaaatcctg
     1381 ccggaagcga actcgcagat tgttggcttc cgctatttca acgtttatgg accgcgtgaa
     1441 ggccataaag gcagcatggc gagcgtcgct ttccatctca acacccagct taataacggt
     1501 gaatcaccga agctgttcga aggtagcgag aactttaaac gcgacttcgt ctatgttggc
     1561 gatgtggcag atgtaaacct gtggttcctg gaaaatggcg tttccggcat cttcaacctc
     1621 ggcaccggtc gtgcggaatc cttccaggct gtagctgatg ctacgctggc ttatcacaag
     1681 aaaggccaga tcgaatacat tccgttcccg gataagctga aaggccgcta ccaggcgttc
     1741 actcaggcag atctgacaaa tctgcgcgcg gcgggttacg acaaaccgtt caaaaccgtt
     1801 gctgaaggtg taacggaata catggcctgg ctgaatcgcg acgcataaga gctctgcatg
     1861 aaaatactgg tgatcggccc gtcttgggtt ggcgacatga tgatgtcgca aagtctctat
     1921 cgcacgctcc aggcgcgcta tccccaggcg ataatcgatg tgatggcacc ggcatggtgc
     1981 cgtccattat tatcgcggat gccggaagtt aacgaagcta ttcctatgcc tctcggtcac
     2041 ggagcgctgg aaatcggcga acgccgcaaa ctgggtcata gtctgcgtga aaagcgctac
     2101 gaccgcgcct acgtcttacc taactccttc aaatccgcat tagtgccttt cttcgcgggt
     2161 attcctcatc gcaccggctg gcgcggcgag atgcgctacg gtttactcaa cgatgtacgc
     2221 gtgctcgata aagaagcctg gccgctaatg gtggaacgct atgtcgcgct ggcctatgac
     2281 aaaggcatta tgcgcacagc acaagatctg ccgcagccat tgttatggcc gcagttgcag
     2341 gtgagcgaag gtgaaaaatc atatacctgt aatcaatttt cgctttcatc agaacgtccg
     2401 atgattggtt tttgcccggg tgcggagttt ggtccggcaa aacgctggcc acactaccac
     2461 tatgcggagc tggcaaagca gctgattgat gaaggttatc aggtggttct gtttggctct
     2521 gcgaaagatc atgaagcagg caatgagatt cttgccgctt tgaataccga gcagcaggca
     2581 tggtgtcgga acctggcggg ggaaacacag cttgatcaag cggttatcct gattgcagcc
     2641 tgtaaagcca ttgtcactaa cgattctggc ctgatgcacg ttgcggcggc gctcaatcgt
     2701 ccgctggttg ccctgtatgg tccgagtagc ccggacttca caccgccgct atcccataaa
     2761 gcgcgcgtga tccgcctgat taccggctat cacaaagtgc gtaaaggtga cgctgcggag
     2821 ggttatcacc agagcttgat cgacattact ccccagcgcg tactgaaaga actcaacgcg
     2881 ctattgttac aagaggaagc ctgacggatg cgggttttga tcgttaaaac atcgtcgatg
     2941 ggcgatgttc tccatacgtt gcccgcactc actgatgccc agcaggcaat cccagaaatt
     3001 aagtttgact gggtggtgga agaagggttc gcacagattc cttcctggca cgctgccgtt
     3061 gagcgagtta ttcctgtggc aatacgtcgc tggcgtaaag cctggttctc ggcccctata
     3121 aaagctgaac gcaaagcgtt tcgtgaagcg ctacaagcag agaactatga cgcagttatc
     3181 gacgctcagg ggctggtaaa aagcgcggcg ctggttacgc gtctggcaca tggcgtaaag
     3241 catggcatgg actggcaaac cgctcgcgaa cctttagcca gcctgtttta caatcgtaag
     3301 catcatattg caaaacagca gcacgccgta gaacgcaccc gcgaactgtt tgccaaaagt
     3361 ttgggctata gcaaaccgca aacccagggc gattatgcta tcgcacagca ttttctgacg
     3421 aacctgccta cagatgctgg cgaatatgcc gtatttcttc atgcgacgac ccgtgatgat
     3481 aaacactggc cggaagaaca ctggcgagaa ttgattggtt tactggctga ttcaggaata
     3541 cggattaaac ttccgtgggg cgcgccgcat gaggaagaac gggcgaaacg actggcggaa
     3601 ggatttgctt atgttgaagt attgccgaag atgagtctgg aaggcgttgc ccgcgtgctg
     3661 gccggggcta aatttgtagt gtcggtggat acggggttaa gccatttaac ggcggcactg
     3721 gatagaccca atatcacggt ttatggacca accgatccgg gattaattgg tgggtatggg
     3781 gagaatcaag tagagtgtcg ttctacaagt atgtctcttg cagatttgcc agctcaaacc
     3841 gtttttcaaa atttaaatct tgaaataata accaataagt tgacatcgga gataagatga
     3901 cctcaacatt atttttctct ctcgagaaaa aaaactggat agcgtactgg aacagagctc
     3961 tcgtattctt attcattacc acctattttt tgggtgggat tacaaggtat aaacatctta
     4021 ttgttattct tatgacaata acgacaatcg tctatctctg caaacggcca aaacactatc
     4081 tctcactatt taaaacattt ctttttggta gtgttgccat attaactatt gctgcattgc
     4141 tgtcacttct tcaatcccct gatgcaggtg ctagcatgaa ggaaattttc aaagctatta
     4201 ttgagaatac tttactatgc acaatagcaa taccgatcat attgagagac gagaaaagag
     4261 aagatgtcga aaaaatcgtt ttcttctcat ttattagtgc gttgggctta cgctgttttt
     4321 ctgaattgat tacctattat aaggactatc aacaagggat aatgccattc gcagattata
     4381 gacaccgtag catttctgac tcgatggtct ttttattccc tgcattgtta aatctctggc
     4441 ttatcaaatc agcaaaatac cgcatttctt ttgtggttct aagcgttatt tttatttttc
     4501 tgatattagg aactttatcc agaggggcct ggctttccgt gttagtcatt ggattaatat
     4561 ggattctgat gtttaaacaa tggaagttac tattagtagg agtaatggtt gccatcattg
     4621 cattgtcggt tattttcaca cataaggaga tgaccgcaaa gctaacgtat aaacttcaac
     4681 aaactaatag ttcttatcgc tatgcaaatg gtactcaagg cagcgcactc gatctaatat
     4741 tagaaaatcc tgttattggt tatggttacg gtaacgttgc atataaagat gtctataata
     4801 aacgtgtcat tgattatcca gaatggacct ttagacaatc aatagggcca cataattttg
     4861 cgctattcat ctggtttggc actggtttat tagggctggt aagtcttatg atgctatact
     4921 gtgcaatatt gaaagagtgt ataaaaaatg gcgtcaagaa taaat
//
LOCUS       DAZFAB010000194         4960 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZFAB010000194 DAZFAB010000000
VERSION     DAZFAB010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4960
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.194"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..845)
                     /locus_tag="ACHJWA_004640"
     CDS             complement(<1..845)
                     /locus_tag="ACHJWA_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO4311089.1"
                     /translation="MAFKHYDVVRAASPSDLAEKLTHKLKEGWQPYGGPVAITPYTLM
                     QAVAIEGDPQVGPSSKPDWFYVVVLAGQSNGMAYGEGLPLPDSYDAPDPRIKQLARRS
                     TVTPGGESCTYNDIIPADHCLHDVQDMSTLNHPKADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGAEGTFSADTGASQDSARWGVGKPLYQDLIARTKAAL
                     QKNPKNVLLAVCWMQGEFDMSAATHAQQPALFTAMLTQFRADLSVFNAQCHGGSAADV
                     PWICG"
     gene            1114..1230
                     /locus_tag="ACHJWA_004641"
                     /pseudo
     CDS             1114..1230
                     /locus_tag="ACHJWA_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1335..1411)
                     /locus_tag="ACHJWA_004642"
     tRNA            complement(1335..1411)
                     /locus_tag="ACHJWA_004642"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1375..1377),aa:Arg,seq:tct)
     gene            complement(1425..1501)
                     /locus_tag="ACHJWA_004643"
     tRNA            complement(1425..1501)
                     /locus_tag="ACHJWA_004643"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1465..1467),aa:Arg,seq:tcg)
     gene            complement(1509..1584)
                     /locus_tag="ACHJWA_004644"
     tRNA            complement(1509..1584)
                     /locus_tag="ACHJWA_004644"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1549..1551),aa:Ile,seq:cat)
     gene            complement(1613..2326)
                     /locus_tag="ACHJWA_004645"
     CDS             complement(1613..2326)
                     /locus_tag="ACHJWA_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4311090.1"
                     /translation="MVVVCSVIMVCSPINIFLEKDTLSLKPGSVVLATKCIRALFLMH
                     YGKVKIFDINHSIVSQYLEIQHKLTRTHLTDVPLYLSLEPNNPALAEALITSQRFSGD
                     TTDMFLMMACLSLFESDERILLFLSGCLSSISAKVRAIIQTDISASWTLGAIALRLHM
                     SESLLKIKLKNEGHMFSRLLLEERMRVAVNMLCSRHGYGQAVAEKCGYSSWSYFISVF
                     HRYYGFPPDRYVSRQGLDY"
     gene            complement(2464..2660)
                     /locus_tag="ACHJWA_004646"
                     /pseudo
     CDS             complement(2464..2660)
                     /locus_tag="ACHJWA_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
     gene            complement(2947..3765)
                     /locus_tag="ACHJWA_004647"
     CDS             complement(2947..3765)
                     /locus_tag="ACHJWA_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFO4311091.1"
                     /translation="MWIVLVLSLSTLSWHKVVAFSLLTVSVVLAVLNDIIDWSVLFFV
                     ATIVFFIILKFNWKYNAWAKSIYEVGIVLSAIALFFHLWPGFHNPVVLNSVTVGPQST
                     PYTMYFNFDKALVPFLLVLCTSSLFKKEVKSEVSLWKWGALSLSVPLILFLAVFFGGL
                     KPEIHFPEWLPEFILANLFFVSLAEESLFRGYIQSRLSEVTSPLVALIVAALLFGFYH
                     YSGGALLVLFATLSGVVYGLSWMWSGRLWVATLFHFGLNLCHLLFFTYPFLKHN"
     gene            complement(3917..4288)
                     /locus_tag="ACHJWA_004648"
     CDS             complement(3917..4288)
                     /locus_tag="ACHJWA_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO4311092.1"
                     /translation="MARDIQMVLERWGAWAANNHEDVTWPSIAAGFKGLIPTKVKSRP
                     KCSDDDAMIICGCMARLNKNNQYLHDLLVDYYVGGMTFMALARKHRCSDGLIGKRLYK
                     AEGIIEGMLMALNVRLDMDMR"
     gene            complement(4278..4649)
                     /locus_tag="ACHJWA_004649"
     CDS             complement(4278..4649)
                     /locus_tag="ACHJWA_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO4311093.1"
                     /translation="MRIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAEVLIDDEQFDEINIVRGQ
                     PVPGGRLGVKIYEIRGGNDGA"
     gene            complement(4662..>4960)
                     /locus_tag="ACHJWA_004650"
     CDS             complement(4662..>4960)
                     /locus_tag="ACHJWA_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO4311094.1"
                     /translation="SFMKRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQG
                     GMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLIFRFLDHAFATGVLG"
BASE COUNT         1465 a         1285 c         1015 g         1195 t
ORIGIN      
        1 tcaccacaaa tccacggcac atctgcagca ctgccaccat ggcactgcgc gttaaacacg
       61 gagaggtcag cacgaaactg tgtcagcatg gctgtaaaca gcgcaggttg ctgtgcgtgg
      121 gtggcggcgc tcatgtcaaa ctctccctgc atccagcaca ccgccagcaa cacatttttc
      181 gggttcttct gtaatgcagc tttagtgcgc gcaatcaggt cctgatataa cggtttaccc
      241 acaccccagc gtgccgaatc ctggctggcc cccgtgtccg cactgaatgt cccctcagcg
      301 ccctgggtga atgcagaacc accacgacag catggtacca gcaggatccc cgcgttattc
      361 gggatatacg gaagcagttt tttggcaata tgtaagccct ggccgacaca gccgtactgc
      421 cctttgctca ggtctgcctt cggatgattc agcgtactca tatcctgcac atcatgcagg
      481 cagtggtcgg ccggaatgat gtcgttatac gtacaactct ctccacccgg agttaccgtg
      541 ctgcggcgcg ccagctgttt aatgcgcgga tccggagcat cgtaagaatc cggtaacgga
      601 agcccttcac cgtaggccat gccgttggac tgtccggcaa gcacaaccac gtagaaccag
      661 tccggcttag atgaagggcc gacctgtgga tctccttcaa tagccaccgc ctgcatcagt
      721 gtgtacggcg taatggcaac cggtccgccg tatggctgcc agccctcttt cagtttgtgt
      781 gtcagctttt ccgcaaggtc tgacggcgac gccgccctga caacatcata gtgtttaaat
      841 gccatgaatc ctcccggccg ggataatatt gtgagtaaaa tgaggagcgg gctgaagtcc
      901 ggaagttaca ggacaatggc agaagagaga caacagcccg caatacgaaa aaggccgcgc
      961 tattgcgcag agtgattact gtcggatatt attcgccagc tgaaatatta cttcacgttt
     1021 tgttgtttat tccttgccgc ccgcgtctcc ctgcgcgggc tttttttgtc cataagaaag
     1081 cccctccgga gaggggctgg agagtggcgc tatgtgccat tgcatggtgc cgggtgcctc
     1141 ccggtgaatt cagtaccagc acctgaatcc gcgattatcc catataccta ctcgctgatt
     1201 gcccctccgc acagggggat tcaccatgcc agtttctttt aacaaactcc ccgcaaacca
     1261 gacaacagtc aaccgcctga attgtgagac atttaaaaaa aaggcccgca aaagcgagcc
     1321 agggaaaata agtgtggcgc gttgtactgg attcgaacca gtgaccgatt gcttagaagg
     1381 caattgctct gtccggctga gctaacaacg caggatacag ataatggacc gccttcgggg
     1441 acccgaactc cgcgcaacca gcttcgaaag ctggcgctct ttcctgatga gctaatggcg
     1501 gtatgtgatg gtggcccttg ctggatttga accagcgacc tggcgattat gagtcgctcg
     1561 ctctcaccac tgagctaaag ggctgggtca aaaaataata atcagatgaa aatcaataat
     1621 caagcccttg cctggataca tatctgtctg gcgggaagcc ataatagcgg tgaaatacag
     1681 aaataaagta ggaccagctt gaataaccgc atttttctgc tacagcctgt ccatatccat
     1741 gccgggaaca taacatattg acagcaacac gcatccgctc ttccagcaac aagcgactga
     1801 acatgtgccc ttcatttttc agttttatct ttaacaaact ctcactcata tgcaggcgta
     1861 acgcaatcgc accaagcgtc cagcttgctg atatatctgt ctgaattatc gccctgactt
     1921 tggcacttat actggataaa catccactta aaaataataa tatccgttca tctgattcaa
     1981 acagcgacag gcatgccatc ataagaaaca tatccgtggt atctccggaa aatctctggc
     2041 tggtaattaa agcctcagcc aacgcagggt tgttgggttc cagtgacaga taaagcggaa
     2101 cgtcagtcag atgagttctt gtcagcttat gctgaatttc cagatattga cttactatgg
     2161 aatggtttat atcgaaaatt ttaactttgc cataatgcat aaggaaaagc gccctgatgc
     2221 atttggtggc cagaacgact gagccgggct taagtgacaa cgtatccttt tcaagaaaaa
     2281 tattaattgg ggagcaaacc atgataactg aacagacaac aaccattata attttacttt
     2341 cattagcaat tggttagttc aattatagcc ccaaaaggta aattatcatc aacacataag
     2401 caaaggactg acaggtgccg ctaacaccca ccagccgccc atttaccaca aataaaaaag
     2461 ccttcaggac tgaaggcgtc tgtaacaacc gaactgatag tctgccagac ccgcataacc
     2521 agctgggtca gtattaactg gcagcgctcg cgtgaaaggt acgtattctg cgcaatctcc
     2581 ccgaccgtcg ccggttcggt gacgcttaat tcatcaaaca caactctggc ggtttctgtc
     2641 atatcctgct gtttcagcat gtctttttac cctttccggt taacgtgaca caccaataac
     2701 tcttgtcgaa aaagccagca agctgaaaga cctgtattaa taactaccag cacatttaac
     2761 gcactgcgct actttgcggg cacaaaaaac ccgctcagag gcggggtcaa gctatgcggc
     2821 gaaataacca ctcttaacag catacctgat tttttacgta cgtaaatgct ttgccgtgca
     2881 tatttttcat gcaaatgtca cgtcctgcta tttttcagtc ttataaattt aaaaccatag
     2941 aaaaaatcaa ttatgtttta aaaatggata ggtaaagaat aacaagtgac acagattcaa
     3001 accaaaatgg aaaagggtgg caacccacaa acgcccactc cacatccatg acaatccata
     3061 cacaacacca gataacgtgg caaataatac aagtaaagca ccacctgaat agtgataaaa
     3121 accaaacaac aaagccgcca caattaatgc aaccaatgga gacgttactt ctgatagccg
     3181 tgattgaata taccctctaa ataatgattc ctctgccaga gacacaaaaa acaaattagc
     3241 caatataaac tctggcaacc actcaggaaa atgaatctct ggctttaatc caccaaaaaa
     3301 aacagccaaa aacaggataa gaggaacaga gagcgacaga gccccccact tccacaaaga
     3361 cacttctgat tttacttctt ttttaaacaa agaagatgta cacaggacta acaaaaatgg
     3421 caccagcgct ttatcaaaat taaaatacat tgtataggga gtactttgag ggccaacagt
     3481 aacagaattt agcactacag gattgtgaaa ccctggccat agatggaaaa ataatgctat
     3541 ggctgataaa actatgccaa cttcatatat agatttagcc caggcgttat atttccagtt
     3601 gaacttcaaa ataataaaaa aaacgattgt agcaacaaaa aataacaccg accaatcaat
     3661 aatatcatta agcacagcca ggacaacaga caccgtcaac aatgaaaaag ccactacctt
     3721 atgccaactg agagttgaca gtgacagtac taacacaatc cacataagca ctccttttat
     3781 ttaatgatga agattgatta tcaatatttt caattcacca ggcaacattt tatctacctt
     3841 ccacaataca tgaccatcaa aaaataaaca tgttaattat aaacacagaa aacataaccc
     3901 tcatcactat atatccctac cgcatatcca tatctaaccg gacattcaga gccataagca
     3961 ttccttcaat aataccttcc gctttataaa gccttttacc aataagccca tcagaacatc
     4021 tatgcttacg tgcaagagcc ataaatgtca ttccacctac gtaataatcc accaacaaat
     4081 cgtgcaaata ctgattattc ttgtttaatc gtgccataca accacaaatt atcatggcgt
     4141 catcatcaga acacttagga cgtgatttca ctttagtcgg gattaatcct ttaaaaccag
     4201 cagctatcga gggccatgtt acatcttcat gattatttgc tgcccatgcc ccccatcgct
     4261 caagaaccat ctggatatca cgcgccatcg ttaccacctc tgatttcgta aatcttcacg
     4321 cccagccgtc cacctggcac aggctgaccg cgcacaatat tgatttcatc aaactgctcg
     4381 tcatcaatga gcacttccgc atgcgtcagc gcatccagcg gtgctttcag aatgttgtcc
     4441 aggtcacggc ggcgcttatc cggtggttct gcaataattt ttattgccag ccgtccggac
     4501 aggcttaatt tcagccgctg ctggcgaaca ataagcgcca cagcccggcg ataacgctca
     4561 ccggcttttg atacaaaata tgtgctgcca cgacgtcgcc agtaggtgtt caccgtcggc
     4621 gggtaaggca aaacaaactc tatacgcatc agtaacctct tttacccgag cacgccggtt
     4681 gcaaaagcgt gatcaagaaa acgaaaaatt aaatcaacct gggaaccatg cttttcttcg
     4741 aacgccagcg gatccgcatg aagctcgttg tgatgctccc gacacagcgg tagcgtgaaa
     4801 atatcgtggg attttgttcc cattccaccc tgaccatgac caatcagatg atgaggatcg
     4861 tccgctggct taccacaaca cgcacacggc tgtgtcttta cccagcgtgt gtatttctca
     4921 tttacccagc ggcgacgttt aggtcgtttc atgaaggatt
//
LOCUS       DAZFAB010000195         4872 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZFAB010000195 DAZFAB010000000
VERSION     DAZFAB010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4872
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.195"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..635
                     /locus_tag="ACHJWA_004651"
     CDS             <1..635
                     /locus_tag="ACHJWA_004651"
                     /inference="COORDINATES: protein motif:HMM:NF013043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO4311095.1"
                     /translation="ILLSIFFMRIKKLIDHDELLSTLSYDSETGIFKWLKTNSVVRVK
                     GSIAGGVSGGYICISINNVLYKAHRLAWFYVYKKWPPKFIDHVNGNRLDNRISNLRLA
                     TEEQNARNIVGNRLNTSGAIGVSWYKPTGRWKSYVGYKNKTISLGYFDSKEDAAFIAA
                     LARKKLYGTYASKALNCEHELLSQFNNDEDKLAEYLKEKYKRTRKRVKKR"
     gene            1099..1431
                     /locus_tag="ACHJWA_004652"
     CDS             1099..1431
                     /locus_tag="ACHJWA_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311096.1"
                     /translation="MDESRKQFEEYVAKKLRLPFEMITEARNGDRYFAFSSMDIRHSL
                     NDWWALWQASRAAIELDIDWPESNDDLWKDGEEGAYAMGYEDGRDKTVIAVMKAIRAA
                     GIKEKNFR"
     gene            1428..1838
                     /locus_tag="ACHJWA_004653"
     CDS             1428..1838
                     /locus_tag="ACHJWA_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFO4311097.1"
                     /translation="MKQTIFLRTKQQQQAAINAILATPLDKDKPVTIRITDYKRNLDQ
                     NAKFHAMLADIARQVQWCGKWLKPEQWKVLLISGHAVATKQEADVLPGLEGEYVNIRE
                     SSAQMSVKRMASLIEYTTAWAIGQGVRFTDRRYE"
     gene            1835..2026
                     /locus_tag="ACHJWA_004654"
     CDS             1835..2026
                     /locus_tag="ACHJWA_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001254209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO4311098.1"
                     /translation="MRRQRRSITDIICENCKYLPTKRSRNKPKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARNTRGLT"
     gene            2292..2624
                     /locus_tag="ACHJWA_004655"
                     /pseudo
     CDS             2292..2624
                     /locus_tag="ACHJWA_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309226.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
     gene            2895..3161
                     /locus_tag="ACHJWA_004656"
                     /pseudo
     CDS             2895..3161
                     /locus_tag="ACHJWA_004656"
                     /inference="COORDINATES: protein motif:HMM:NF022018.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
     gene            3258..3563
                     /locus_tag="ACHJWA_004657"
                     /pseudo
     CDS             3258..3563
                     /locus_tag="ACHJWA_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309584.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="P22AR C-terminal domain-containing protein"
     gene            3638..4360
                     /locus_tag="ACHJWA_004658"
     CDS             3638..4360
                     /locus_tag="ACHJWA_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor protein"
                     /protein_id="HFO4311099.1"
                     /translation="MNELINSNVIKMTSIEISELTGKRHDNVKRTIETLAKNGVIRHP
                     QIEDCGRINGLGLNQSFRVYVFEGEQGKRDSIIVVAQLSPEFTARLVDRWRELEGATA
                     KIPQTFSEALRLAADLEDQKAELEKQLALAAPKVEFADRVGEASGILIGNFAKVVGIG
                     PNKRFAWMRDHKILIASGARRNVPMQEYMDRGYFTVKETAVNTNHGIQISFTTKITGR
                     GQQWLTRKLLDNGMLKVTGEAA"
     gene            4360..>4872
                     /locus_tag="ACHJWA_004659"
     CDS             4360..>4872
                     /locus_tag="ACHJWA_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO4311100.1"
                     /translation="MAKPARRKCKICKEWFHPAFSNQWWCSPEHGTKLALERRNKERE
                     KAEKTAEKKRRREEQKQKDKIKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCISC
                     GTLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELINRIGQE
                     AVDEIESNHNR"
BASE COUNT         1551 a         1020 c         1159 g         1142 t
ORIGIN      
        1 atatattatt aagtatattc tttatgcgaa taaaaaaact aattgatcac gatgagcttc
       61 tgtcaacatt atcatatgac tcagaaacag gaatatttaa atggctaaaa acaaattcag
      121 tagtaagagt aaaaggtagt attgctggag gtgttagtgg tggttatata tgcattagta
      181 taaataatgt tttgtataag gcgcatagac ttgcttggtt ctatgtatac aaaaaatggc
      241 ctcctaagtt tattgatcat gtaaatggga acagacttga taataggatt tcaaacctaa
      301 gactggcaac agaagagcag aatgcaagaa acattgtagg gaatagatta aacacatccg
      361 gtgcaattgg agtgtcttgg tataagccaa ctggcaggtg gaagtcttat gttggttata
      421 aaaataagac aatatcgtta gggtatttcg atagcaaaga agatgcagca ttcatagcag
      481 cactagcaag aaagaaacta tatggaactt atgcgagtaa agcacttaat tgcgagcatg
      541 agcttttatc tcaatttaat aatgatgagg ataaacttgc ggaatatctt aaggaaaaat
      601 ataaaaggac tcgaaagcgt gttaaaaaaa gataaaggcc tgctggtaat cgcaggcctt
      661 tttatttggg ggagagggaa gtcatgaatc tggacaagca agatgcacaa actattagct
      721 catacataag ggcatcaaga ccagattaca aaggtccggt attcgtagat ttatctcgcc
      781 ttgaatagat ttacatgtgg aaagcaaggt tacttacgca ttttgttatt cgaacgatga
      841 ctagcaacat tacaaaacca atgtaactgg aagaggtgaa tatgagcaca ctcgcagacc
      901 ttattcatgc cgatatggct gaagatggag caaggcgtaa taggtactgg aaatcatcga
      961 gccttccagt ttgtgaaaga ttcaatcaca ggccaaaacc aaaacgtagc cgacgagaca
     1021 aagtgttgaa aaaactcatg caaattaaca tggctggttt tgtcagattc gtgagtgaaa
     1081 cgactaacgg tgattgatat ggacgaatca agaaagcagt ttgaggaata cgttgccaaa
     1141 aaattgagat taccattcga gatgataacc gaggcaagaa atggtgatag gtacttcgca
     1201 ttttcaagca tggatattcg tcactcctta aatgattggt gggctttatg gcaggcatcg
     1261 cgagcagcta ttgaactgga tatcgactgg cccgaatcga atgacgacct ttggaaagat
     1321 ggtgaagaag gtgcttatgc gatgggttat gaggatgggc gtgacaaaac ggtaattgca
     1381 gtaatgaaag ctatcagagc cgctggaatt aaagagaaga attttcgatg aagcaaacaa
     1441 tcttcctccg aactaagcaa caacagcaag ctgcaataaa tgccatcctc gcaacaccac
     1501 tcgataaaga caagccagtc accatccgca ttactgacta caagcgcaac cttgaccaga
     1561 acgcaaaatt tcacgcgatg ctggcggata tcgctcgtca ggttcaatgg tgcggcaaat
     1621 ggttaaaacc agaacaatgg aaggttttgt tgatcagcgg tcatgcagtg gcaacaaagc
     1681 aggaagctga tgttttgccc gggcttgaag gcgaatacgt caacattcgc gaaagcagcg
     1741 cgcagatgag tgtgaagcgt atggcaagtc tgattgagta cacgacagca tgggctattg
     1801 gtcagggtgt cagatttacc gacaggaggt acgaatgaga cgacagcgac gaagtatcac
     1861 cgacataatc tgtgaaaact gcaaatacct tccaacgaaa cgctccagaa ataaacccaa
     1921 gccaatccca aaagaatctg acgtaaaaac cttcaactac acggctcacc tgtgggatat
     1981 ccggtggcta agacatcgtg cgaggaatac aagggggttg acgtgatgat ttatccgggg
     2041 ctatattcct cacacgccag caaaatctgg cgtcgggatt ggcgtcctgg atagagaccg
     2101 cgacagatac acgccgcgag cgtgtttttt attgtcgtat gcacgcgcac atctgaatta
     2161 tggtgggctg tgtgggggcg gagagatccg cgccggtcgg tttcccggtt acgccaaccc
     2221 tgcacagttc accaccagac gattggcgtc gtcggtggtg agttattaag aaaccactcg
     2281 agggcgtcat tatgacaact caaatctctg ttgaaactct ctccccgatc acccataacc
     2341 agattcctgt tattaccacc gaacttttgg cgcagttgta cggtgctgaa gttaagaaca
     2401 ttcaaaataa ctacgccaga aatgctgagc gctttattga aggaaagcat tttttcaaag
     2461 tggctggcga tgccctgaaa aatttgcggg ttactttaag taactcacaa aatttgcaac
     2521 catctttaag aggtttgcaa atttccccca aaactcgctc cctcatcctc tggacagaac
     2581 gaggcgcagc ccgtcacgcc aaaatgctcg aaaccgatca ggcttgacgt tataggttgt
     2641 tacttgtaga tttatcttcg tgaggcgtca gaacctctct aaagcggaca aacccaaccc
     2701 cgatagtgtt ggatttttta tgcctgtcat tttatggacg cattgcgcgg tcataccccg
     2761 aacaatgtcg ggagggcgac gaatacaaca cccgaaaggg gaatgagtcc gcggttcttt
     2821 aggccgttct gaacctcccg acaccacaat cagaagtggt aattcagaaa aagctaaagg
     2881 aggtcgttat gaccatgcaa ctcgccgtag aatccctcgt cgttgttact tactcgaaca
     2941 ttcccgtaat aacaacggag ttattggcaa cactatacaa aacagaacaa aagcatatac
     3001 ggcaaaattt caaacgtaac gaatgtcgtt ttattgcagg aaaacacttc tttaaagtaa
     3061 gtggtagtga actcgatgat ttgagaacct ctcaaagagg tttgcaaatt tcccccaaaa
     3121 ctcgctccct catcctctgg acagaacgag gcgcagcccg tcacgccaaa atgctagaaa
     3181 ccgatcaggc gtgggaagtg ttcgaaaaac tggaagactg ctatttcagc cagacttcga
     3241 aaagcgcagg tcatcacgag aagaaaacca gcgagctttc tgcaaaagat gcaaacagtc
     3301 tggtatggtt gtgggattat gccaaccgct cacaggcatt gttccgcgaa ctgtatccgg
     3361 cgctaaaaca aattcaatcg aactattccg gcagatgcta cgactacggc catgagtttt
     3421 cgtatgttat cggaatggcg agagatgttt taatcaatca cacacgagat gttgatatta
     3481 atgatccaga cggaccaacg aatctttccg catgggtaag acttaagaac aaagaattac
     3541 ctcctgcact gcatcgctac tgacaattga caacttaaca aacccagctt cggctgggtt
     3601 ttttattggt gaattttcaa tatgagagga catgacaatg aacgagctga taaatagcaa
     3661 cgtcatcaaa atgacgagca ttgaaatctc tgagcttaca ggtaagcgtc atgacaatgt
     3721 gaaacgtact atcgaaacgc tggctaaaaa tggtgttatc cggcatcctc aaattgagga
     3781 ttgtggaaga atcaatgggt taggcttaaa tcaaagtttt cgtgtgtatg tattcgaagg
     3841 cgaacaaggt aagcgcgata gcatcattgt tgtcgctcag ttgtcgccgg aattcaccgc
     3901 tcgccttgtt gaccgctggc gagaactcga aggggcaacc gcgaaaatac cacaaacctt
     3961 ttctgaggca ttgcgccttg cggccgacct tgaagaccag aaggctgaac tggagaaaca
     4021 gcttgctctc gcagcaccta aagttgagtt tgccgatcga gttggcgagg ccagcggaat
     4081 tttgattgga aactttgcaa aggttgttgg tattggtcca aacaaacggt ttgcgtggat
     4141 gcgcgatcac aaaatcctta ttgcttcagg tgcccggcgc aatgtgccaa tgcaggaata
     4201 tatggatcgc ggctatttca cagtgaaaga aacagcggtc aatacaaatc acggaataca
     4261 gatatcgttc accacaaaaa tcaccgggcg tggtcaacag tggctgacaa gaaagctgct
     4321 agataacgga atgcttaaag taacagggga ggctgcttaa tggctaaacc agcgcgaagg
     4381 aaatgcaaaa tctgtaagga atggtttcac ccggcattct caaatcagtg gtggtgcagc
     4441 ccggaacacg gaactaaatt agcactcgaa cgacgaaata aagaacgcga aaaggcggaa
     4501 aaaacagcag agaagaaacg acgacgagag gagcagaaac agaaagataa aattaagatt
     4561 cgaaaactcg ccttaaagcc ccgcagttac tggattaaac aagcccaaca agccgtaaac
     4621 gccttcatca gagaaagaga ccgcgactta ccatgtatct cgtgcggaac gctcacgtct
     4681 gctcagtggg atgccggaca ttaccggaca actgctgcgg cacctcaact ccgatttgat
     4741 gaacgcaata ttcacaagca atgcgtggtg tgcaaccagc acaaaagcgg aaatctcgtt
     4801 ccgtatcgcg tcgaactgat taatcgcatc gggcaggaag cagtagacga aatcgaatca
     4861 aaccataacc gc
//
LOCUS       DAZFAB010000196         4738 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZFAB010000196 DAZFAB010000000
VERSION     DAZFAB010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4738
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.196"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            70..213
                     /locus_tag="ACHJWA_004660"
     CDS             70..213
                     /locus_tag="ACHJWA_004660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311101.1"
                     /translation="MNNFSKTSTMQIKRIKPSIIYRLLLIVLGIPMVIYGLVCPLTIE
                     TRD"
     gene            complement(432..1970)
                     /gene="aldB"
                     /locus_tag="ACHJWA_004661"
     CDS             complement(432..1970)
                     /gene="aldB"
                     /locus_tag="ACHJWA_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFO4311102.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKNRYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAEAWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(2078..3373)
                     /locus_tag="ACHJWA_004662"
     CDS             complement(2078..3373)
                     /locus_tag="ACHJWA_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFO4311103.1"
                     /translation="MAIKSPPTLIPLSHLSGEELQAHLRFNRVTDEKGRYLPFDELQY
                     RIKKGENVDVAWTLTRLARNAAIQRINYCNEAGEQAGFNITPVIAEACELVDKRATAL
                     ALKDQTERLRGAGAELSQLRLEEPITSSQLEGANTTTLVARKMLETGRSPRTEDEHMI
                     AGNARLMAEIPHLLAEPLTPALIRQLHAIGMGGINDAKYRPGEFRETDDVVIADYDGN
                     IVHQPPAAALLPERLEKVCQWLNSHEGYIHPLVRACILHFMLAHEHPFRDGNGRTSRA
                     LFYWYMLKSGYDVFKYISISRLLHAAPVKYAASYQYTESDGMDLTYFLEYQAGVIKRA
                     LQNWQQHIDEITQRSAKLDSVLFSSGVLKRLNPRQVTLLNVMLANPGKEYTVAEISAS
                     LGVSDNTVRADLRTIVKEGFAQEKKINDQQAVYFAHYPL"
     gene            complement(3503..4654)
                     /gene="yiaY"
                     /locus_tag="ACHJWA_004663"
     CDS             complement(3503..4654)
                     /gene="yiaY"
                     /locus_tag="ACHJWA_004663"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFO4311104.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLAANALKDACGFTNPIQATHEEIVAIYRAAM"
BASE COUNT         1143 a         1256 c         1151 g         1188 t
ORIGIN      
        1 gtgtgctgcg tggcttttta acaggtcgag gcttatcata ttgaaataat ggaactatca
       61 gccaaactaa tgaataactt cagtaaaaca tccacaatgc agattaagcg tattaaacct
      121 tcaataatct accgtttatt gctgattgtc ctcggaatac caatggtgat ttacggtctg
      181 gtttgcccgc tcaccatcga aacgcgagat taaactcctg gcaaaacacg ccccagaaac
      241 gcacttttgt tggcgtagtc tgggttattg cacacgtagg tttaaaacgt aaattgtaac
      301 aacgtgcgct ttgtttatgc cggatgcggc gtgtacgcct tatccggcct acggtcaggt
      361 ccccataggc atgataagac gcgtaagcgt cgcatcaggc aattaatacc cgatgcgacc
      421 agcttcttac atcagaacag ccccaacggt ttatctgagt agctcactag caggcacttg
      481 gtttgctggt aatgctccag catcatcttg tgggtttcgc gaccgatacc tgattgtttg
      541 tagccaccaa acgccgcatg tgccgggtaa gcatgataac agttggtcca cacgcgccca
      601 gcctgtatgc cgcgccccat cttataggcc agattaccgt tgcggctcca gacgcccgcg
      661 cccaggccat attgcgtatc gttcgccagc tccagcgctt cttccatcgt tttgaaggta
      721 gtcaccgcca gcaccgggcc aaaaatctcc tcctggaaca cgcgcatatt gttctgacca
      781 aacagaatcg tcggttcgag gtagtagccg tctttcagtt caccttccag cagcttgcgc
      841 cgcccgccgg tgagcacatc agcgccctct tttttaccga tatcaatgta gttgaggatg
      901 gtttccagtt gcccgtgaga aacctgcgca cccatttgcg tcacgctgtc gagtgggtta
      961 ccgctgcgaa tgctttcgac acggcggatg gcgcgttcca taaagcgttc gtagattgac
     1021 tcctgcacta aggcacgact cggacaggtg caaacttcgc cctggttaaa ggcaaacagt
     1081 gcaaagcctt ccagcgcttt atcgaaaaag gcatcttctt catccatcac atcagcaaag
     1141 aagatatttg gcgacttacc gcccagctcc agcgtcaccg gaataatgtt ttgcgtggcg
     1201 tattgcataa tttgttgacc cacttccgtt gagccggtaa acgccacttt ggcgatgcgt
     1261 ttcgaggtcg ccagatattc gccaatttcc ccacctgcac cgttgaccac gttcaccacg
     1321 cccggcggca gtaaatcacc gacgatttcc attagcagca gtacagaaag cggggtaaga
     1381 cgtgcgggtt tcagcaccac acagttgccc gccgccagcg cgggagccat tttccagctc
     1441 gccatcagca gcgggaagtt ccacgggata atctgcccca ccacgcctaa cggttcatgg
     1501 aaatgatagg ccacggtttc gctatcaact tcactgatcc caccttcctg cgcccgaata
     1561 cacgaggcga aatagcggaa atggtcaatc gccagcggta catccgcagc actggtttcg
     1621 cgaatgggtt tgccgttatc ccaggcttca gctgtcgcta acagctcgag gttttgttcc
     1681 attcgatcgg caatcttaaa cagaatcgcc gcgcgatcct gcaccgaggt gtgcgcccat
     1741 ttatctttca ctttgtgcgc agcatccagc gccagatcga tgtctcgttt gcccgaagac
     1801 gccacttcgc acagcagctg cccggtcacc ggcgtcaggt tctggtaata ctcaccatcg
     1861 gcaggggcta cccattcgcc gccaataaag ttgtcatagc ggttttttaa cttgaggggg
     1921 aaaccatact cgccgggctt aatctgtgct gaagggggat tattggtcat gatcgtctcc
     1981 ttgcggtgtg aggtataaca agggtagacg tggctggcga aatcttcgcc agtcggtaac
     2041 agcttttcga cggctatcac gaatttacgg gcaagggtta cagcggataa tgcgcgaagt
     2101 acacggcttg ctggtcatta attttcttct cttgcgcaaa cccttcttta acaattgtcc
     2161 gtaaatcagc tcgaaccgta ttatcagaga cgcccaggct cgcgctaatt tcagcaactg
     2221 tgtactcttt ccccggattg gcgagcatca cattcaggag cgttacctgg cgcggattca
     2281 ggcgtttaag cacaccagag ctaaacagca cactatcgag ttttgcgctt cgttgtgtaa
     2341 tttcatcgat atgctgctgc cagttttgca gagcacgttt aatgacaccc gcctgatatt
     2401 caaggaaata agtcagatcc ataccatctg attcagtgta ctggtagctg gcggcatatt
     2461 tgaccggtgc agcatgaagc aaacggctga tggaaatata tttaaagaca tcgtagcccg
     2521 atttcagcat ataccagtaa aacagcgccc gactggtgcg cccgttgcca tcccgaaacg
     2581 gatgttcatg ggccagcata aaatgcagaa tgcaggcacg gaccaacgga tgaatatacc
     2641 cttcatggct gtttaaccac tggcaaactt tttccagccg ttccggtaac aatgccgcag
     2701 cgggcggttg atgcactata ttgccatcat agtcggcgat cacaacatca tctgtttcac
     2761 gaaattctcc cggacgatat ttcgcgtcat tgattccacc cataccaatg gcatgtaact
     2821 ggcgaataag cgcaggtgtc agcggttctg cgagtaagtg cggaatttcc gccatcagcc
     2881 gggcatttcc cgcaatcata tgctcgtctt cagtgcgtgg cgaccgtcct gtttccagca
     2941 tttttctcgc caccagcgtt gtagtattcg caccttccag ctgggagctg gtgatcggct
     3001 cttccaggcg taactggctt aactcggctc cggccccgcg taaccgttca gtctgatctt
     3061 tcagcgccag cgcggtagca cgtttgtcga caagctcgca cgcttctgca atcaccgggg
     3121 taatgttgaa tccggcctgt tcacccgctt cattgcaata attaattcgt tggatcgccg
     3181 cgttgcgcgc cagacgggta agcgtccacg ccacatcgac gttttctcct tttttgatgc
     3241 ggtactgcaa ctcatcaaaa ggcagataac gtcctttttc atcggtaacc cggttaaagc
     3301 gcaggtgggc ctggagttcc tcacccgata agtgtgataa aggaatcagc gtgggaggtg
     3361 atttgatagc catttgctta tctcaaaaaa gcgcaatatt gagatagtgt atagcaggaa
     3421 agagaaacta tctcaaaaaa agcaagtttt gagatagttg aaaaaatggc tgacaatttg
     3481 ccagccgttg tggaaatgat gattacatcg ctgcgcgata aatcgccaca atttcttcgt
     3541 gagttgcctg gatcgggtta gtaaagccac aggcatcttt cagggcatta gccgccagaa
     3601 ccgcgaaatc ttcttctttc acgttcaggt cgcgtaggcc tgccgggata tccactttct
     3661 tcgccagttc acggatggcg ttaatgcagg cttcagcacc ttccgcgtcg tttttacctg
     3721 tcacgttcac gcccattgca gcggcacagt cacgcagacg tgcagcggcg actttgctgt
     3781 tgaatacctg aacatgcggc agcagaacgg cgttacatac accgtgtggc aggttgtaga
     3841 aaccgcccag ctggtgcgcc atcgcatgaa cataacccag agaagcatta ttgaacgcca
     3901 taccggcgag gaactgggca taagccattg cttcacgcgc tttcgcatta ctgccatctt
     3961 caacggctaa cggcaggttt tcggcaatca tggtcacggc tttcagtgca caagcgtcag
     4021 tgatcggcgt ggcggcaata gaaacatacg cttcgatagc gtgcgttaag gcatccatac
     4081 ccgttgcggc ggtcagtgac ttcggcatac caatcatcag agaggagtca ttgacagaaa
     4141 gcagcggagt gacatgttta tcaacaatcg ccattttgat atgacgcgct tcgtcagtga
     4201 tgatgcagaa acgggtcatt tcagaggccg tacccgccgt ggtattgatg gcgatcatcg
     4261 gcagctgcgg ttttgcagag cggtcaacgc cttcgtaatc gcgaatatcg ccgccattgg
     4321 ctgccaccag cgcaatacct tttgcgcagt cgtgtggaga accaccgcct aaggagatca
     4381 cgctatcgca attattctct ttaagtaatt tcaaacctgc ggcgacgttt tccgtggtgg
     4441 ggttaggttg ggtgccatca taaataacgc taaaaatatt gcgttcttcc agtgcttttt
     4501 gcacatcgcc cgccatacct aatttcgtta acatactgtc agtgacaatt aaggtacggg
     4561 taaatccata atctgccatc atattcattg catcagtcaa tgaatcagcg ccgatgacat
     4621 tcacagaagg aataaagaaa gttgaagatg ccataacaca ctcctgaaag tgtaaaaaag
     4681 ggtgggcagc ttactgagga ttttcattcg aaaacatgat cattattatt aattatca
//
LOCUS       DAZFAB010000197         4673 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZFAB010000197 DAZFAB010000000
VERSION     DAZFAB010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4673
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.197"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(139..354)
                     /locus_tag="ACHJWA_004664"
                     /pseudo
     CDS             complement(139..354)
                     /locus_tag="ACHJWA_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(398..964)
                     /locus_tag="ACHJWA_004665"
                     /pseudo
     CDS             complement(398..964)
                     /locus_tag="ACHJWA_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
     gene            complement(976..1380)
                     /locus_tag="ACHJWA_004666"
                     /pseudo
     CDS             complement(976..1380)
                     /locus_tag="ACHJWA_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(1380..1565)
                     /locus_tag="ACHJWA_004667"
     CDS             complement(1380..1565)
                     /locus_tag="ACHJWA_004667"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311105.1"
                     /translation="MDVRNKKLVFWFVRVDDEGYPEIARCTEREFATILAGISAGGMY
                     CPECGTVHWPDGVPPPF"
     gene            1742..3952
                     /gene="katP"
                     /locus_tag="ACHJWA_004668"
     CDS             1742..3952
                     /gene="katP"
                     /locus_tag="ACHJWA_004668"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase KatP"
                     /protein_id="HFO4311106.1"
                     /translation="MIKKTLPVLILLALSGSFSTAVAADKKETQNFYYPETLDLTPLR
                     LHSPESNPWGADFDYATRFQQLDMEALKKDIKDLLTTSQDWWPADYGHYGPFFIRMAW
                     HGAGTYRTYDGRGGASGGQQRFEPLNSWPDNVNLDKARRLLWPVKKKYGSSISWGDLM
                     VLTGNVALESMGFKTLGFAGGREDDWESDLVYWGPDNKPLADNRDKNGKLQKPLAATQ
                     MGLIYVNPEGPGGKPDPLASAKDIREAFSRMAMDDEETVALIAGGHTFGKAHGAASPE
                     KCIGAGPDGAPVEEQGLGWKNKCGTGNGKYTITSGLEGAWSTSPTQFTMQYLKNLYKY
                     EWELHKSPAGAYQWKPKKAANIVQDAHDPSVLHPLMMFTTDIALKVDPEYKKITTRFL
                     NDPKAFEQAFARAWFKLTHRDMGPAARYLGNEVPAESFIWQDPLPAADYTMIDGKDIK
                     SLKEQVMDLGIPASELIKTAWASASTFRVTDYRGGNNGARIRLQPEINWEVNEPEKLK
                     KVLASLTSLQREFNKKQSDGKKVSLADLIVLSGNAAIEDAARKAGVELEIPFTPGRTD
                     ASQEQTDVASFSVLEPTADGFRNYYSKSRSHISPVESLIDKASQLDLTVPEMTALLGG
                     LRVMDINTNNSSLGVFTDTPGVLDNKFFVNLLDMSTRWSKADKEDTYNGFDRKTGALK
                     WKASSVDLIFSSNPELRAVAEVYASDDARNKFIHDFVKSWNKVMNSDRFDLNNK"
     gene            3996..4385
                     /locus_tag="ACHJWA_004669"
     CDS             3996..4385
                     /locus_tag="ACHJWA_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFO4311107.1"
                     /translation="MQKSNKVIISCGMALYFLCGGITVAEELDESMQVLARNMSVLQS
                     SMDKKELLEALDRMDDAVDESMKRLPESISPADEEGKNDYIKELQKLEKEIMLAKQKV
                     LSGQLSDIPDSVNKMNKIRVEGHDKFR"
     gene            4442..4673
                     /locus_tag="ACHJWA_004670"
                     /pseudo
     CDS             4442..4673
                     /locus_tag="ACHJWA_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    4498..4614
                     /locus_tag="ACHJWA_004670"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT         1282 a         1043 c         1175 g         1173 t
ORIGIN      
        1 aaaaagaaca gatatgcgag ttatgatctt gatcgatagg aatttaaatc ccaaaaagat
       61 taaaaaaaca cccaaaaacg gatgtttatt caacactgcc tgcgtgccat gagtgcaccg
      121 ccgtcaatta aatggatcta tttcatcctg accagcatag tgctccagac ggctcatcgg
      181 caccggcggt tttttcaggt aagagccgaa gtacaccgcc acatgtgtgg cgttatccat
      241 cacccgggat acgttgacat tccagccccg ccggtaatga ctgtccagga agcgattcca
      301 gtcccgttta ctgcttcctt ctgctgccag cgcatccggt atcaccaggt ccggtgaggt
      361 gtactggcaa tagcggacac taccatttgt tcttttttta agcagccatc tgatgatatt
      421 tttccctgaa ggctgccggg gagatattcc ccagacgaga gtgacgacgc tgacgattgt
      481 agaaaatctc aatgtattcc cgtattactg agatggcttc atcccggtta ttaaaacgat
      541 agtggctcag gctctcattt ttcagcgttc cccagaagct ttccatcgga gcgttgtcgt
      601 aacagttacc tttacgcgac attgatgttt tcagaccaaa ctgctcctgt atgacccggt
      661 aatcgtatgc gcagtactgt gaacctcgat cagagtggtg gattagcccg gcaggtgggc
      721 gctggctcct gagcgccata aacagggctt tacctgtcag ctcttttgtc atgcgctctc
      781 ccatggcgta gccgacaatt tcgcacgtat aaacatcttt gatgccagcg aggtacaacc
      841 atccctcctg tgtggcaaca tacgtcaggt ccgccaccca gacctgattt ggtgctgcag
      901 gagcgaacgt ctggttcagc agatttggcg caactggcag attgtggttc gagttcgtag
      961 tcgcacatct gcctagcggc agatttcctg tatcacatcc gcagcaatgc ggctcatctc
     1021 tgccagtaac caccggttgt ggaacaccag ggaccagtac tggcagggaa gtgtgaacac
     1081 aatatgctgc cacggacagt cgggaaccag actcagcaga tactgtatcc actgtgcgcc
     1141 agccttcact ccgcagtgcg ggcaggagcg acttttacac cggaagcaga ccttttttat
     1201 gtggctgcag tccggagatg aacagcacca ctgtgtgtac cccatcagcg tggtcccgca
     1261 ggccatgatt tttgtcacag actcaatgac taccggacgc actgaacctt ccggttgttt
     1321 ctccagccag ttaagccagc ggtttccctg ctgaaaaatg tcggcaaaac ggggaagcat
     1381 cagaagggcg ggggaactcc gtccggccag tgaaccgtgc cacactccgg gcagtacatg
     1441 ccgccggcgc tgataccggc aagaatggtc gcaaactccc gctccgtgca gcgggctatt
     1501 tcaggatacc cttcgtcatc aacacgtaca aaccagaaga ccagcttttt gtttctgaca
     1561 tccacaaaga agggaatatt caggtctgcg cagcactcaa cggcatcgtc agttgcggct
     1621 tggaacccct tagtattttt tgtctgtagt atctatccca gcaataggta tatcctgttg
     1681 catcaataaa gttgactttt gtatacaaca tgcgaatttc ccttaatccg gagctattcg
     1741 tatgataaaa aaaactcttc ctgttctgat tcttctggcg ctatcgggga gcttttctac
     1801 cgctgtagcc gctgataaaa aagagactca aaatttctac tatccagaaa cactggattt
     1861 aactcctctg agattacaca gccctgaatc aaatccctgg ggggctgatt ttgattatgc
     1921 caccagattt caacagctgg atatggaggc tctgaaaaaa gatatcaaag atttgctgac
     1981 aacttcccag gattggtggc ctgcggatta tggtcattat ggtcctttct ttattcgtat
     2041 ggcttggcac ggtgccggaa catacaggac atatgatggc cggggaggcg ccagtggtgg
     2101 tcagcaacgt tttgaaccgc tgaacagctg gccggataac gttaatctgg ataaagcccg
     2161 tcgattgctg tggccagtca agaaaaaata cggctccagt atttcctggg gagacctgat
     2221 ggtcctgact ggtaatgttg cccttgaatc catgggattt aaaacgctgg gatttgctgg
     2281 cggaagagaa gatgactggg agtcggacct ggtatactgg gggcctgaca acaagcctct
     2341 tgcagataac cgggataaaa acgggaaact tcagaaacct cttgccgcca cgcagatggg
     2401 acttatttat gtcaatcctg aaggccccgg tggaaaacca gatcctctgg cttccgcgaa
     2461 agatatcagg gaagcttttt cacgtatggc catggatgat gaggagactg tggccctgat
     2521 cgcgggaggg catacatttg gtaaagcaca tggtgcagcg tctcctgaaa aatgtattgg
     2581 cgcagggcct gatggtgcac ctgtggagga gcagggactg ggatggaaaa ataaatgtgg
     2641 tacaggaaac ggcaaatata ccatcaccag tggcctggaa ggagcctggt cgacatcgcc
     2701 aacccagttc acaatgcagt atctgaagaa tttatataaa tatgaatggg agctgcacaa
     2761 gagtcctgcc ggtgcttatc agtggaagcc taaaaaagcg gcaaatatag ttcaggacgc
     2821 gcatgatccg tctgtcctgc atccgttgat gatgtttacg acggatattg ctcttaaagt
     2881 tgatcctgaa tataagaaaa taaccacccg tttcctgaat gatccaaaag cttttgagca
     2941 ggcattcgca agagcatggt ttaaactgac ccaccgggat atgggaccgg cagcccgata
     3001 tcttggtaat gaagttcctg cagaatcatt tatctggcag gatcctcttc ctgcggcgga
     3061 ttatacaatg attgatggta aagacattaa gtcgctgaaa gagcaggtta tggatttggg
     3121 tatccctgca tctgagctga taaagacagc ctgggcttca gcttccacat ttcgtgtgac
     3181 tgattatcgt gggggaaata atggtgcccg catcaggtta cagcccgaaa ttaactggga
     3241 agttaatgag cctgaaaaac tgaagaaagt actggcatcc ctgacctcat tacagcgtga
     3301 atttaacaaa aaacagtctg acggaaagaa agtgtcgttg gctgatttaa ttgttctttc
     3361 gggtaatgct gcaatcgaag atgcggccag aaaagccggg gtggaacttg agattccctt
     3421 tactccggga agaactgacg cctctcagga gcagacggat gttgcctcat tcagtgtact
     3481 ggagccgaca gcagatggat tcagaaatta ttactcaaaa agcagaagtc atatatcgcc
     3541 ggttgaaagc ctcattgata aagccagtca gctggatctc accgttcctg aaatgacggc
     3601 attactgggt ggtctgcggg taatggatat taatacaaat aattcttcgt tgggagtgtt
     3661 taccgatacc cctggtgttc tggataacaa gttttttgtt aatctgctgg atatgtcaac
     3721 acgatggagt aaagcagata aagaagatac atacaatgga ttcgatcgta aaacgggagc
     3781 attaaaatgg aaagcatcct ctgttgattt aatcttcagt tcaaatcctg aattacgtgc
     3841 ggtggcagaa gtatatgcct cggatgatgc gagaaataag tttattcatg attttgttaa
     3901 atcgtggaat aaagttatga atagcgatcg gtttgattta aacaataaat gataaaactg
     3961 atgagggagg gcacctccct catgaaggag aaattatgca gaaaagcaat aaagttataa
     4021 tcagctgtgg tatggcatta tattttttgt gtggggggat tacagtggca gaagaacttg
     4081 atgaaagcat gcaagttctt gcaaggaata tgagtgtact tcagtcatca atggataaaa
     4141 aggagcttct tgaagctctt gacagaatgg atgatgctgt agatgagtca atgaagcgtc
     4201 ttcctgagag catttcaccg gctgatgaag aagggaagaa tgactatata aaagaacttc
     4261 aaaagcttga aaaagagatt atgctggcga aacaaaaagt cttatcggga cagttatcag
     4321 atatacccga ttcggtaaac aagatgaata aaatcagagt tgaggggcat gataaattca
     4381 gataaatcag ttggttgatt cctgcgggac gaatacatac gtcaggaaca taactttatg
     4441 gctggaacgt gaaaatcgtg aactgcgccg cagtaacgat atccttcgcc aggcttccgc
     4501 ttattttgcg aaggcggagt tcgaccgcct ctggaaaaaa taatgccact gctggataag
     4561 ctgcgtgagc agtacggggt cggaccggta tgcagtgagc tgcatattgc cccgtcaacg
     4621 tattaccact gtcagcaaca gcgacatcat cctgataaac gcagtgcccg tgc
//
LOCUS       DAZFAB010000198         4647 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZFAB010000198 DAZFAB010000000
VERSION     DAZFAB010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.198"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..143
                     /locus_tag="ACHJWA_004671"
     CDS             <1..143
                     /locus_tag="ACHJWA_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFO4311108.1"
                     /translation="GKNETITTPRLSFRFLNVSPTVERQLQRIIFSLEREAREKADKV
                     RD"
     gene            complement(145..756)
                     /gene="emtA"
                     /locus_tag="ACHJWA_004672"
     CDS             complement(145..756)
                     /gene="emtA"
                     /locus_tag="ACHJWA_004672"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFO4311109.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            856..1770
                     /gene="ldcA"
                     /locus_tag="ACHJWA_004673"
     CDS             856..1770
                     /gene="ldcA"
                     /locus_tag="ACHJWA_004673"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFO4311110.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQYDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNTFTEHHFWLALRNETFTIEWQ
                     GEGPTCRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILTNTREGTQLTISGHPVLKM"
     gene            1866..3602
                     /gene="cvrA"
                     /locus_tag="ACHJWA_004674"
     CDS             1866..3602
                     /gene="cvrA"
                     /locus_tag="ACHJWA_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFO4311111.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(3760..>4647)
                     /locus_tag="ACHJWA_004675"
     CDS             complement(3760..>4647)
                     /locus_tag="ACHJWA_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001366955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO4311112.1"
                     /translation="MPLLDKLREQYGVGPVCSELHIAPSTYYHCQQQRHHPDKRSARA
                     QRDDWLKREIQRVYDENHQVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRGK
                     KVRTTVSRKAVSAGDRVNRQFVAERPDQLWVADFTYVSTWQGFVYVAFIIDVFSGCIV
                     GWRVSSSMETTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYTERLKEAKLLASTG
                     STGDSYDNAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRRLLGRLGHIP
                     PAEAEKAYYASIRNDDLAA"
     misc_feature    complement(4576..>4647)
                     /locus_tag="ACHJWA_004675"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
BASE COUNT          989 a         1213 c         1209 g         1236 t
ORIGIN      
        1 atggcaagaa tgaaaccatc accactcccc gtctgagctt ccgttttctt aacgtcagcc
       61 cgacggtgga gcggcaatta cagcggatta ttttctctct cgagcgggaa gcccgggaga
      121 aagcggacaa agtacgcgac tgatttacat cgcgtccagt gcctgctcaa gtttgtagat
      181 atagcgcgga gcctgcggcg caggatgatt tcgcgctacg tgatcgagga actcgtcagc
      241 atccagatcg ttgattttgc tgattgcctt tttccgatct gacgagaaag tccgtagcag
      301 cgcacctgcc ccgttagcat atgacaccac cagcgcatat tgcagtacct tcggatcttc
      361 aatgcctgcc agcgggccgg tttccagaat attcaggtaa gccgccccca tagaaatatt
      421 acgctccgga tttttcagct cgctggtcgt cggctcacca ctccagccca tacgccgata
      481 aacatcacgt ccggaggttg aagcttttaa ctgcatcaaa ccaatggcat tcgatttact
      541 caccgcgttc ggattaccac ccgattcgat agcgataatc gccgtgatca attgtggatc
      601 gacgccccag gctgcaccgg ctttctggct tattggcatc cactgcatcg cacgttgcac
      661 cggaactttc gcgttccacg gcgggttcgt atagtcatgc tttgatgaac aacccgctaa
      721 taacacaatc aaaaaggcaa accatctcaa tttcacttct tctatcctta tagcctggaa
      781 tcgccgttga ggtgacaacg gcacacctaa gcggcatgat atacatttcg tatctgttgt
      841 cagcaaggaa ttgccatgtc tctgtttcac ttaattgccc catcgggtta ctgcattaaa
      901 cagcacgccg cgctgcgtgg tatccagcgc cttaccgacg cggggcatca agtcaataac
      961 gtcgaggtta ttgcccgtcg ttgtgagcgt tttgcgggta cggaaacgga gcgtctggaa
     1021 gatctcaatt cccttgccag actgactacc cccaacacca tcgtgctggc tgtacgcggc
     1081 ggttacggtg ccagtcgttt actggcagat attgactggc aggcactggt ggctcgccaa
     1141 caatatgacc cgttgctcat atgcgggcat agcgatttta ccgccattca gtgcggtctt
     1201 ctggcgcaag gcaatgtcat cacctttagc ggccccatgc tggtagcaaa ctttggcgcg
     1261 gatgaactga acacctttac cgagcaccat ttctggctgg cgttacgcaa tgaaaccttt
     1321 accattgaat ggcaagggga aggcccgaca tgtcgggccg aaggcacgtt gtggggaggc
     1381 aatcttgcga tgctgatttc actgatcggt acaccgtgga tgccaaaaat tgagaacggt
     1441 attctggtgc tggaagatat taacgagcac cctttccgcg tcgaacgtat gctgttgcag
     1501 ctctatcatg cgggaatttt gccacgtcag aaggcgatta ttctcggtag ctttagcggc
     1561 agtacgccta atgattatga cgcaggttac aacctggagt cagtgtacgc atttttgcgt
     1621 tcccgcctgt cgattccact cattaccggc ctcgattttg gtcatgaaca acgcacggtt
     1681 acgctgccgt tgggcgcaca tgccattctt actaacaccc gggaaggcac tcagttgacg
     1741 attagcggtc atcctgttct taaaatgtaa aaaaaatcac gcttcagggc taagtaaaac
     1801 gctgtctctg ccgctaatat ggtagggttt atataagaga cagcgtaatc aggagtaacc
     1861 gaccgttgga tgccacaaca ataattagcc ttttcatctt aggatccatc cttgttacca
     1921 gcagtatatt acttagttca ttttcttctc gtcttggcat tcctattctg gttatttttc
     1981 tggcgatcgg catgctggcg ggagtcgatg gcgtcggcgg tatcccattt gataattacc
     2041 ccttcgccta catggtaagt aacctggcgc tggcgattat tttgctcgat ggcgggatgc
     2101 gcactcaggc cagttccttt cgtgtggcgt taggaccggc gctgtcgctg gcgacgctgg
     2161 gcgtacttat cacctctggt ttaaccggca tgatggcggc gtggctgttt aatcttgatt
     2221 tgattgaagg cttattaatc ggcgctatcg tcggctccac cgatgctgca gcggtctttt
     2281 ctctgctggg tggtaagggg cttaacgaac gtgttggctc gacgctggaa attgaatccg
     2341 gcagtaatga tccaatggcg gtctttctga cgattaccct gattgcgatg atccagcagc
     2401 atgaaagcag tgtcagttgg atgttcgtgg tcgatattct gcagcaattt ggcctcggga
     2461 ttgtcatcgg tcttggcggt ggttatttac tgctgcaaat gattaatcgc atcgccctgc
     2521 ccgccggttt atatccattg ctggcattaa gcggcggtat tttaattttt gctttaacca
     2581 cggcgctgga aggtagcggt attctggctg tttatctgtg cggttttctg ctgggtaatc
     2641 ggccgattcg caaccgctac ggcatcctgc aaaatttcga cggcctcgcc tggctggcgc
     2701 aaatcgccat gttcctggtg ctggggctgt tggttaaccc aagcgatctg ctgcctattg
     2761 ccattccggc gctcattttg tccgcatgga tgattttctt cgcccgtcct ctttcggtat
     2821 ttgccggatt gctaccattc cgtggcttca atctgcgtga gcgcgtgttt atcagctggg
     2881 tgggattacg cggcgcggtg ccgattattc tggccgtgtt cccgatgatg gcggggctgg
     2941 agaatgcacg tctgttcttt aatgtcgcct tctttgtggt tctggtttca ctgctcttgc
     3001 agggaacatc actctcgtgg gcggcgaaaa aagccaaagt ggtggttccg cccgtcggac
     3061 gtccggtgtc acgcgttggc ctggatattc atccggaaaa tccgtgggag cagtttgttt
     3121 atcaattgag tgccgataaa tggtgcgtgg gcgcggcact gcgcgatttg catatgccaa
     3181 aagagacgcg tattgcggca ctgtttcgtg ataaccagtt gcttcatcct accggcagca
     3241 cccgactgcg cgaaggcgat gtgttgtgtg taattggtcg ggaacgcgat ctcccggcgc
     3301 tcggtaaact gttcagccag tcgccgccgg tcgcgctgga tcaacgcttc tttggtgact
     3361 tcattctcga agccagcgcc aaatatgcag atgtggcgct gatatatggc ctggaagacg
     3421 ggcgagaata tcgtgataag caacaaacgc tgggtgaaat tgtccagcag ttgttaggcg
     3481 cggcaccggt tgtcggtgac caggtagagt ttgccgggat gatctggacg gtggccgaga
     3541 aagaagataa tgaagtgttg aagattggcg ttcgagtggc tgaagaggaa gctgaatctt
     3601 aaatttaatg aagcatctat cgccggttac gatgctttgc ttaacgcagc ctgagcctgt
     3661 atataaactt gtcatttcct gattttgata tgcacattcc aacatcagaa gaaggtttga
     3721 accgccccgg ttttcctgga gagtgtttta tctgtgaact caggctgcca gatcatcgtt
     3781 tctgatggaa gcataataag ctttttctgc ttctgccgga gggatatgac ccagccttcc
     3841 cagcaatcgt cgattgttat accagtccac ccacgtgagt gtggccagtt ccacttctgc
     3901 acggtttttc cagctcttac ggtgtattac ctccgctttg taaagaccat tgatgctctc
     3961 cgccatcgcg ttgtcatacg agtcacctgt actccctgtt gatgccagca gttttgcttc
     4021 ttttagtcgc tccgtatagg ccagtgatac atactgagaa cctttatcac tgtgatggac
     4081 tgtgccggac ggccgacggg cccacaacgc ctgctccagt gcatccagca cgaatgtcgt
     4141 ttccatagac gatgagactc gccaccccac gatacatccg gaaaacacat caatgatgaa
     4201 cgccacatag acgaagccct gccatgtgct gacgtaagta aaatcagcca cccacagctg
     4261 gtcaggacgt tctgccacga actgacggtt tacgcggtcg cctgcggaaa cggctttccg
     4321 gctgacggta gtacggacct ttttaccccg gagaacaccg gcaagtccca taaccgccat
     4381 gaggcgcgcc actgtacatc tggccaccct gataccttcg cgtaacaact ggcgccagac
     4441 tttacgcaca ccgtacacct gatgattttc atcgtatacg cgctgtatct ctctcttcag
     4501 ccagtcatcg cgctgcgcac gggcactgcg tttatccgga tgatgtcgct gttgctgaca
     4561 gtggtaatac gttgacgggg caatatgcag ttcactgcat accggtccga ccccgtactg
     4621 ctcacgcagc ttatccagca gcggcat
//
LOCUS       DAZFAB010000199         4643 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZFAB010000199 DAZFAB010000000
VERSION     DAZFAB010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.199"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..191
                     /gene="ylcI"
                     /locus_tag="ACHJWA_004676"
     CDS             <1..191
                     /gene="ylcI"
                     /locus_tag="ACHJWA_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO4311113.1"
                     /translation="TKNRTRRTTTRNIRFPNQMIEQINIALDQKGSGNFSAWVIEACR
                     RRLTSEKRAYTSIKSDEE"
     gene            complement(356..562)
                     /gene="ybcW"
                     /locus_tag="ACHJWA_004677"
     CDS             complement(356..562)
                     /gene="ybcW"
                     /locus_tag="ACHJWA_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbcW"
                     /protein_id="HFO4311114.1"
                     /translation="MNKEQSADDPSVDLIRVKNMLNSTISMSYPDVVIACIEHKVSLE
                     AFRAIEAALVKHDNNMKDYSLVVD"
     gene            848..1258
                     /gene="ybcV"
                     /locus_tag="ACHJWA_004678"
     CDS             848..1258
                     /gene="ybcV"
                     /locus_tag="ACHJWA_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFO4311115.1"
                     /translation="MAQVAIFKEIFDQVRKDLNCELFYSELKRHNVSHYIYYLATDNI
                     HIVLENDNTVLIKGLKKVVNVKFSRNTHLIETSYDRLKSREITFQQYRENLAKAGVFR
                     WVTNIHEHKRYYYAFDNSLLFTESIQNTTQIFPR"
     gene            1550..1843
                     /gene="bor"
                     /locus_tag="ACHJWA_004679"
     CDS             1550..1843
                     /gene="bor"
                     /locus_tag="ACHJWA_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serum resistance lipoprotein Bor"
                     /protein_id="HFO4311116.1"
                     /translation="MKKMLLATALALLITGCAQQTFTVQNKPAAVTPKETITHHFFVS
                     GIGQKKTVDAAKICGGAENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSQ"
     gene            complement(1934..2116)
                     /gene="rzoD"
                     /locus_tag="ACHJWA_004680"
     CDS             complement(1934..2116)
                     /gene="rzoD"
                     /locus_tag="ACHJWA_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HFO4311117.1"
                     /translation="MRKLKMMLCMMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(2333..2809)
                     /locus_tag="ACHJWA_004681"
     CDS             complement(2333..2809)
                     /locus_tag="ACHJWA_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFO4311118.1"
                     /translation="MQNLNPQRKAFLDMLAWSEGTDNGRQPTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLHPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVRESEV"
     gene            complement(2796..3101)
                     /locus_tag="ACHJWA_004682"
     CDS             complement(2796..3101)
                     /locus_tag="ACHJWA_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO4311119.1"
                     /translation="MHEKESLAGAFWLVLLIIAGWGGLVRYLIDVKQSKATWSWINAL
                     AQIVVSGFTGVIGGLISIESGFSIYMILATAGISGAMGSVALTYFWERLTGVKNAKS"
     gene            complement(3423..4112)
                     /locus_tag="ACHJWA_004683"
     CDS             complement(3423..4112)
                     /locus_tag="ACHJWA_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFO4311120.1"
                     /translation="MNTQYLQYVREQLIVATADLSGATKGQLEAWLEHAQFDTGTYKR
                     KKPRILDEVTGRMITLDNPPISGKQSYAKGSSIALVSQVEFSTSSWRRAVLSLEEHQK
                     AWLLWSYSESVRWEHQVTITQWAWSEFKTLLGTRKIAGKTLERLKKLIWLAAQDVKNE
                     LAGRKTYEYQELASLVGVTSKNWSETFTERWVAMKHIFLQLDSQALLLLTKTRSKQKT
                     TFSQQDIAKLD"
     gene            complement(4109..4249)
                     /locus_tag="ACHJWA_004684"
     CDS             complement(4109..4249)
                     /locus_tag="ACHJWA_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFO4311121.1"
                     /translation="MMFESYMAERLRHRWMRLRLYRFPGSVLTDYRILKNYAKTLKGA
                     AA"
     gene            complement(4246..4608)
                     /gene="rusA"
                     /locus_tag="ACHJWA_004685"
     CDS             complement(4246..4608)
                     /gene="rusA"
                     /locus_tag="ACHJWA_004685"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFO4311122.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVARIIKG
                     SMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGKLELTITELGNE"
BASE COUNT         1274 a         1132 c          990 g         1247 t
ORIGIN      
        1 caacgaagaa cagaacccgc agaacaacaa cccgcaacat ccgctttcct aaccaaatga
       61 ttgaacaaat taacatcgct cttgatcaaa aagggtccgg aaatttctca gcctgggtca
      121 ttgaagcctg ccgtcggagg ctaacgtcag aaaagagagc atatacatca attaaaagtg
      181 atgaagaatg aacatcccgc gttcttccct ccgaacagga cgatattgta aattcactta
      241 attacgaggg cattgcagta attgagttgc agttttacca ctttcctgac agtgacagac
      301 tgcgtgttgg ctctgtcaca ggttaagtag tttgaatgat tagcagttat ggtgatcagt
      361 caaccaccag ggaataatcc ttcatattat tatcgtgctt caccaacgct gcctcaattg
      421 ccctgaatgc ttccagagac accttatgtt ctatacatgc aattacaaca tcagggtaac
      481 tcatagaaat ggtgctatta agcatatttt ttacacgaat cagatccacg gagggatcat
      541 cagcagattg ttctttattc attttgtcgc tccatgcgct tgctcttcat ctagcggtta
      601 aaatattact tcaaatcttt ctgtatgaag atttgagcac gttggcctta catacatctg
      661 tcggttgtat ttccctccag aatgccagca ggaccgcact ttgttacgca accaatacta
      721 ttaattgaaa acattcctaa tatttgacat aaatcatcaa caaaacacaa agaggtcaga
      781 ccagattgaa acgataaaaa cgataatgca aactacgcgc cctcgtatca catggaaggt
      841 tttaccaatg gctcaggttg ccatttttaa agaaatattc gatcaagtgc gaaaagattt
      901 aaactgtgaa ttgttttatt ctgaactaaa acgtcacaat gtctcacatt atatttacta
      961 tctagccaca gataatattc acattgtgtt agaaaacgat aacactgtgt taataaaagg
     1021 acttaaaaag gttgtgaatg ttaaattctc aagaaatacg catcttatag aaacgtccta
     1081 tgataggttg aaatcaagag aaatcacatt tcagcaatac agggaaaatc ttgctaaagc
     1141 aggagttttc cgatgggtta caaatatcca cgaacataaa agatattact atgcctttga
     1201 taattcatta ctatttactg agagcattca gaacactaca caaatctttc cacgctaaat
     1261 catcacgtcc ggtttcttcc gtgtcagcac cggggcgttg gcataatgca atacgtgtac
     1321 gcgctaaacc ctgtgtgcat cgttattaat tattcccgga cactcccgca gaggagtttc
     1381 cctgtcaggg ctgcggacat agttaatccg ggaatacagt gacgatcctt cgcatctgac
     1441 atacattaat aaatattaac aatatgagat ttcaactcat tgtttagggt ttgtttaatt
     1501 ttctacacat acgattctgc gaactttaaa aagcatcggg aataacacca tgaaaaaaat
     1561 gctactcgct actgcgctgg ccctgcttat tacaggatgt gcccaacaga cgtttactgt
     1621 tcaaaacaaa ccggcagcag taacaccaaa ggaaaccatc acccaccatt tcttcgtctc
     1681 tggaattggg cagaagaaaa ctgtcgatgc agctaaaatt tgtggcggcg cagaaaatgt
     1741 tgttaaaaca gaaacccagc aaacattcgt aaatggattg ctcggtttta ttactttagg
     1801 catttatact ccgctggaag cgcgtgtgta ttgctcacaa taattgcatg agctgcccat
     1861 cgatatgggc agctctatct gcactgctca ttaatatact tctgggttcc ttccagttgt
     1921 ttttgcatag tgatcagcct ctctctgagg gtgaaataat cccgttcagc ggtgtctgcc
     1981 agtcgggggg aggctgcatt atccacgccg gaggcggtgg tggcttcacg cactgactga
     2041 cagactgctt tgatgtgcaa ccgacgacga ccagcggcaa catcatcatg cagagcatca
     2101 ttttcagctt tcgcatcagc taactccttc gtgtattttg catcgagcgc agcaacatca
     2161 cgctgacgca tctgcatgtc agtaattgcc acgttcgcca gcttcagttc tctggcattt
     2221 ttgtcgcgct gggctttgta ggtaatggcg ttatcacggt aatgattaac agcccatgac
     2281 aggcagacga tgatgcagat gagcagagcg gagataatcg cggttattct gctcatacct
     2341 cactctctct gaccgttccg ccagcttctt tgaattttgc aatcaggctg tcagccttat
     2401 gctcgaactg accataacca gcgcccggca gtgaagccca gatattactg caacggtcga
     2461 ttgcctgacg gatatcaccg cgatcaatca taggtaaagc accacgctct ttaatctgct
     2521 gcaatgccac tgcgtcctgg cttttggggg agaagtcttt caaaccaagc tgtttacggt
     2581 aagcatccca ccagcgtgaa agaagctgat aacgtccggc ggctgttgat ttgagtttgg
     2641 ggtgtagcgt gacaagtttg cgagggtgat cggagtaatc agtaaacagt tcgccgccaa
     2701 caataacatc ataaccgtgg ttacgtgtcg gttgtcgccc gttatccgtt ccttctgacc
     2761 acgccaacat atcgaggaag gctttacgct gaggattaag attttgcatt tttcacccct
     2821 gtcagtcgtt cccagaagta cgtcagtgca accgaaccca tcgcaccact aatccccgct
     2881 gtcgcgagaa tcatgtaaat actgaatcca ctttcgatgc tgatcaggcc accaataaca
     2941 ccggtgaatc ctgataccac tatttgagcc agagcattta tccaactcca cgttgcttta
     3001 ctctgcttca catctatcag gtagcggacc agaccgcccc aacctgcgat gatcagcaaa
     3061 acgagccaga acgctccggc aaggctctct ttttcgtgca tatgaatagc caatgtttcg
     3121 ccgccgacaa aaggccggga cgttaaatgt cagaaatcag gctcacgggg taatttaacg
     3181 acaaagcacg gagttgatgc tccccacaag cctggaataa aaaagccagc atgtagctgg
     3241 caacagaggg ctaagcaata tcaactcaac agctgaagac cccctggctg gggtaggttg
     3301 gaaggctact caccgttcag aaacagaaaa gcccaaggct ttaaacctcg ggcttgaatt
     3361 tggattactg ccagtgcgta caacattggc aaaatatcag atttacataa aatatatgat
     3421 ttttaatcca gttttgcaat atcttgctgt gaaaatgtgg tcttttgttt tgaacgtgtt
     3481 ttcgttaaaa gcaataaagc ttggctatca agctgtagaa aaatgtgttt cattgcaacc
     3541 cagcgttcag taaatgtctc agaccagttt tttgatgtca ctcccaccag tgatgccagc
     3601 tcctggtatt cataggtctt acgccctgcc agctcgttct tcacatcctg tgccgccagc
     3661 cagatcaact tcttcaaacg ttccagtgtc ttacctgcaa ttttcctggt acccaacaga
     3721 gtcttaaact cgctccatgc ccactgcgtt atggtgacct gatgttccca gcgaacactt
     3781 tcgctgtaac tccacagcaa ccacgctttc tgatgttctt cgagagacag aaccgcgcgg
     3841 cgccatgacg aggttgagaa ctcaacctgg ctgaccagtg caatggatga accttttgcg
     3901 tacgactgct taccggaaat cggcggatta tccagcgtaa tcatcctgcc agttacctca
     3961 tccagaatgc gcggcttctt tcgtttgtat gtaccagtat caaattgtgc atgctccagc
     4021 caggcttcaa gctgtccttt cgttgctccg ctcaaatcag cggtagccac aatgagttgc
     4081 tcgcggacat actgtaaata ttgggtattc atgcggcagc tcctttcagt gttttggcgt
     4141 aattcttcag tatccggtaa tcggtcaaaa cagaaccagg aaaacgatat aagcgcaggc
     4201 gcatccagcg gtggcgaaga cgttctgcca tataagactc aaacatcatt cattccccag
     4261 ttcggtgatg gtcagctcca gcttcccacc tttggtaacg ggcatcttca caacacggta
     4321 atcaacgacc tgagcatcat ccagccagaa acctgctttg gtgagtgcgt caaaagcggc
     4381 tttttgcaga ttatccaggt cacggcgacg gcgatccggc atgtggcact caatacggat
     4441 tttcactggc atagccaggc cgatatccag catggagcct ttgatgattc gggcgacgtt
     4501 atcacggtat gcctgccctt ctgcgctgat gtgcgtgcgc ccgcgattat gccggtagta
     4561 gcgattattg ctcggaggcc agggtaatgt gatgctgtag gtattcacgc cttaataacc
     4621 ccctctttca gccagataac ctg
//
LOCUS       DAZFAB010000200         4610 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZFAB010000200 DAZFAB010000000
VERSION     DAZFAB010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4610
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.200"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..1269)
                     /locus_tag="ACHJWA_004686"
     CDS             complement(<1..1269)
                     /locus_tag="ACHJWA_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO4311123.1"
                     /translation="MTFKHYDVVRAASPSDLAERLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVAVTGNEGKAVAVSATRAPEYYFVVVLAGQSNGMSYGEGLPLPETY
                     DRPEPRIKQLARRSTVTPGGAACRYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGT
                     VGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGADGTYSDASGASENSTRWGVDK
                     PLYKDLIGRTKAALKKNPKNVLFAVVWMQGEFDFGGTPANHAAQFGALVDKFRADLAD
                     MAGQCVGGSADGVPWICGDTTYFWKQKNEATYQTVYGSYKNKTEKNIHFVPFMTDENG
                     VNVPTNKPEEDPDIPGIGYYGSKWRDSSATWTSQDRASHFSAWARRGIISDRLATAIL
                     RHAGRVALNAGASSTVSEVRPSSPSGAEATG"
     gene            1513..1836
                     /locus_tag="ACHJWA_004687"
     CDS             1513..1836
                     /locus_tag="ACHJWA_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFO4311124.1"
                     /translation="MNWIVIDTVIQPACGISFSAIWCNIKLILWYQSDTFLPPGCIFT
                     PTRTGVILNNKELPVTIYNVAPFNKNVWNLIKNSQECPANTGNITDKCFNHRCILEIC
                     PYGRK"
     gene            complement(2133..2402)
                     /locus_tag="ACHJWA_004688"
     CDS             complement(2133..2402)
                     /locus_tag="ACHJWA_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2c subunit B"
                     /protein_id="HFO4311125.1"
                     /translation="MKKMFMAVLFALVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
     gene            complement(2414..3373)
                     /locus_tag="ACHJWA_004689"
     CDS             complement(2414..3373)
                     /locus_tag="ACHJWA_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HFO4311126.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     DSQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            complement(3466..3542)
                     /locus_tag="ACHJWA_004690"
     tRNA            complement(3466..3542)
                     /locus_tag="ACHJWA_004690"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3506..3508),aa:Arg,seq:tct)
     gene            complement(3556..3632)
                     /locus_tag="ACHJWA_004691"
     tRNA            complement(3556..3632)
                     /locus_tag="ACHJWA_004691"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3596..3598),aa:Arg,seq:tcg)
     gene            complement(3634..3709)
                     /locus_tag="ACHJWA_004692"
     tRNA            complement(3634..3709)
                     /locus_tag="ACHJWA_004692"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3674..3676),aa:Ile,seq:cat)
     gene            complement(4023..4511)
                     /locus_tag="ACHJWA_004693"
     CDS             complement(4023..4511)
                     /locus_tag="ACHJWA_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO4311127.1"
                     /translation="MGIRELNLTKEQHEWLNGWLELWGAWVYSGRLEKRMSSVIAKFM
                     ESVEPGRVMTRPMCNDDDGMLISQVVDSVMYIDKKAFGILLSYYAHGSSKHAIASYYH
                     SVARPRKMLCRGGGRIQKPSLATCRREVDEILNASLFMIYPILDSAFKNRKRVEKIKH
                     VA"
     gene            complement(4502..>4610)
                     /locus_tag="ACHJWA_004694"
     CDS             complement(4502..>4610)
                     /locus_tag="ACHJWA_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311128.1"
                     /translation="AVKPLKFANQMYIDTGAVFCGNLTLIQVQGEGAWA"
BASE COUNT         1225 a         1217 c          986 g         1182 t
ORIGIN      
        1 gcctgtggct tctgcaccgg aaggcgatga cgggcgcacc tctgatactg tcgatgatgc
       61 ccccgcgttt agcgccactc ttcccgcatg gcgcaaaatc gccgttgcca gacggtcgga
      121 aataatccca cggcgtgccc aggcgctgaa atggctcgcc ctgtcctgtg acgtccaggt
      181 ggctgagctg tcacgccatt tcgaaccgta atatccgata cccggaatgt ccgggtcttc
      241 ttccggtttg ttcgtcggca cattcacccc gttctcatcc gtcatgaacg gtacgaaatg
      301 gatatttttt tccgttttgt ttttgtagct gccgtacacc gtctggtagg tggcttcgtt
      361 cttctgcttc cagaaatacg tcgtgtcccc gcatatccag ggaacaccgt cagcagagcc
      421 accgacgcac tgacctgcca tatccgccag gtctgcacgg aatttatcaa ccagcgcacc
      481 aaactgtgct gcgtgatttg ccggcgtacc gccaaaatca aattccccct gcatccacac
      541 cacggcaaac agcacatttt tcgggttctt cttcagtgct gcttttgttc gaccgataag
      601 gtccttatac agcggcttgt ccacccccca gcgggttgaa ttctccgaag caccactcgc
      661 gtcactgtat gtgccatcag ctccggtggt gaacgctgaa ccaccacgac agcacggaac
      721 cagcagaatg cccgcattcg ccggtataaa cggcagcaat tttttggcga tatgcagccc
      781 ctgccccacg gttccgtact gcccctttga caggtccgct ttcggatggt taagacggct
      841 catgtcctgc acatcatgca gacaatggtc cgccggaatg atgtcgttat atctgcatgc
      901 tgcaccaccc ggtgtcaccg tactgcgacg cgccagttgc ttaatacgcg gctccggacg
      961 gtcatatgtc tccggcagcg gaagaccttc accatacgac atgccgtttg actgccctgc
     1021 cagaaccaca acaaagtaat actccggggc tctggtggca ctgacagcca ccgccttacc
     1081 ctcattaccg gtcaccgcca ctggtgtggt gacatcacct tccgccgcaa tggcctgcat
     1141 cagggtgtaa ggcgtgatgg ccaccggact gccaaatggc tgccagccct ccttcagttt
     1201 ttgtgtcagt cgttcagcaa ggtctgacgg cgatgccgcc ctgaccacat cgtagtgttt
     1261 aaatgtcata acctctcctg ccgccagcaa agtaattatc cggaacacac aatcatgtaa
     1321 cacataaaaa acacgctata aaaaaaccat gtctcttata taacaaaaaa acgccgacaa
     1381 tttaaaatgc cctcttattt taaacaaaga ataaacgaca ccagaaaaac aactcagcct
     1441 gacagattgc tttcaaaaca tcacctataa tttatggcat catttaaaac cttaagaaaa
     1501 taaggaaagc aaatgaactg gatagtgatt gacacggtaa tccagccagc atgtggcata
     1561 tcattttcag ccatatggtg taacataaaa ttaatattat ggtatcagtc agataccttc
     1621 ctccctccgg gctgtatttt tacaccaacc cggacaggcg taatactcaa taataaagag
     1681 ctccccgtca ccatttacaa cgtggcccca tttaataaaa acgtctggaa tttaattaaa
     1741 aacagccagg agtgccctgc aaacacagga aatatcacag ataagtgttt taatcatcgc
     1801 tgcattctgg aaatatgccc atacgggcga aaataacaga atcattcaga taaaaagccc
     1861 ctccggagag gggctgacac tgcgtatctg tatcatcatg tacatggtgc cgggtgcctc
     1921 ccggtgagtt cagtccggtg tccctgaacc cgcgtatctc gctccaggtt gtcgtcagag
     1981 atgacacctt atacaccagt cgcccctccg cacaggggga ttcaccatgc gaattttttt
     2041 aacgaatgct cagtctgaca ggcaactgtc aactgactga attgtgacac agattacact
     2101 tgttacccac ataccacgaa tcaggttatg cctcagtcat tattaaactg cacttcagca
     2161 aatccggagc ctgattcaca ggtactggat ttgattgtga cagtcattcc tgtcaactga
     2221 gcactttgca gtaacggttg cagattccag cgactggtcc agtactcttt tccggccact
     2281 tttactgtga atgtatcatt ctcattatac ttggaaaact caattttacc tttagcgcaa
     2341 tccgccgcca ttgcattaac agaaactaat gcaaataaaa ccgccataaa catcttcttc
     2401 atacttaact cctttattta cccgttgtat ataaaaactg tgactttctg ttcagaaacg
     2461 ctgcagctgt attactttcc cataatgtat tgtttatttt tataacgggc ctgtcgccag
     2521 ttatctgaca ttctggttga ctatcttcat tcacggcgcg aacagaacgc gccccctgat
     2581 gatgacaatt cagtataacg gccacagtgc ccagtatcgc cgatatatta ttaaaggata
     2641 ttctccccac tctgacacca tcctctcccc gatactccgg aagcacattg ctgattcgcc
     2701 cccagttcag agtgaggtcc acgtctcccg gcgtcatcgt atacacagga gcagtttcag
     2761 acagtgcctg acgaaattct ctctgtatct gcctgaagcg taaggcttct gctgtgacag
     2821 tgacaaaacg cagaactgct ctggatgcat ctctggtcat tgtattacca ctgaactcca
     2881 ttaacgccag atatgatgaa accagtgagt gacgactgat ttgcattccg gaacgttcca
     2941 gcgctgcgac acgttgcaga gtggtataac tgctgtccgt tgtcatggaa accgttgtca
     3001 caccgggcac tgatatatgt gtaaaatctg aaaaacggta gaaagtattt gttgccgtat
     3061 taacgaaccc ggccacatat aaattatttt gctcaataat cagacgaaga tggtcaaaac
     3121 gcgcctgata gacatcaagc cctcgtatat ccacagcaaa ataactgccc ggtggggtgt
     3181 ggttaataac agacaccgat gtggtcccct gagatatatg ttcaagaggg gtcgatatct
     3241 ctgtccgtat actatttaac gaagatacat aactttgttg agtcgaaaag tctatcgtaa
     3301 attcccggga ataggatacc gaagaaaagc ccagtaacag gcacagtacc catttaaata
     3361 atatacactt catatacaag gtgttccttt tggctgaagt aatcagcacc agacccggcg
     3421 cagatataaa aaaggcccgc aaaagcgagc cagggaaaat aagtgtggcg cgttgtactg
     3481 gattcgaacc agtgaccgat tgcttagaag gcaattgctc tgtcctgctg agctaacaac
     3541 gcagaatacc gataatggac caccaccggg gactcgaacc tcgcgcaacc agcttcgaag
     3601 gctgacgctc tatcccgatg agctaatggc ggttggtggc ccttgctgga tttgaaccag
     3661 cgacctggcg attatgagtc gctcgctctc accactgagc taaagggccg agcgtaggat
     3721 aataacgtta cgaaatcaat gttgcaagag ttcacaaatt cactgattca aaattattct
     3781 tgtaaaaaat cttttaacgt caggcacaaa gagcctgtac atagatttgt gtaattgcct
     3841 gattttgata tgttcaatcc agcatcaaat gaaggttaat ttatggacga aaaacagtta
     3901 caggctctaa tgacctttat acgattgacc caaataaaaa aagccaccgt tgcaacttaa
     3961 gagtcactaa cggcagctta tgcgaatagt gttgctcatt tgctcaacga tgtcaacacg
     4021 ttctatgcta catgcttaat tttctctaca cgtttccggt ttttaaacgc actatccaga
     4081 atcgggtaaa tcataaacaa cgaggcattg aggatttcgt caacttcccg tcggcaggtt
     4141 gctagcgatg gtttttgaat gcgcccgccg ccccggcata acatcttgcg aggtcttgcg
     4201 acgctatgat agtaagatgc aatggcgtgc ttggaagagc cgtgggcgta gtagctgagg
     4261 aggatgccaa aggctttctt gtcaatgtac atgacggaat cgacgacctg agaaatcaac
     4321 attccatcat catcattaca cattggcctt gtcataactc ttcccggctc cacgctctcc
     4381 atgaatttag ctattacgct gctcatgcgc ttttccagac gacctgaata aacccatgcg
     4441 ccccacagtt caagccagcc attcagccac tcatgctgtt ctttggtgag gtttagttct
     4501 cttatgccca cgcgccttct ccctgtacct gaatcaatgt gagatttccg cagaacactg
     4561 cgccggtatc gatatacatc tggttggcaa atttgagtgg tttcactgct
//
LOCUS       DAZFAB010000201         4565 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZFAB010000201 DAZFAB010000000
VERSION     DAZFAB010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.201"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            2..1156
                     /locus_tag="ACHJWA_004695"
     CDS             2..1156
                     /locus_tag="ACHJWA_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4311129.1"
                     /translation="MILDKKSNLPTYYQNLFLTTDIRNRGATASTMEIVATNLLIFSN
                     FLDGRGIDIIERVELKKYLSVAEIDDLVRYAKQRFDRQKIINIKSTNYRFIAKRTFSY
                     RIHVFSRYLKWLCGLVHSSKGIHAKYEVDVFIESIRAHIPRNSSLNMNEISEKSLNEE
                     EIKVLFRLLEIGGIENPFHKEVQVRNRLIFTLLLNLGLRAGELLNLKIDDFDLRDNTL
                     SVVRRHDSKEDGRSYQPLVKTGERVIPLSDELAREIFDYISNSREKMTKRKKHNFLFV
                     AHCTGKNAGEPLSISAYEKVISTLKRASPELYNLSGHRLRHSWNYMYSKGIEGAELEY
                     NRRKDLRNYLMGWSKESIMCDRYNYKYISQQEKDVVLKVYKSVSKIISGV"
     gene            1158..2471
                     /locus_tag="ACHJWA_004696"
     CDS             1158..2471
                     /locus_tag="ACHJWA_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4311130.1"
                     /translation="MENKCIESEQIFFAKMNRYSFKLSDKKWQLDKENCVYPHKVVDR
                     MPTKMKLSYLKTLAYYASEYSSFYIQSINNLFYEWFGAMTIDTIDDKAIYQLNVYLGS
                     ERNYKLNLIKAFIIKWKNLNYPGVEATAIRMLEKIKIIPNQTGDAVKRQDPNKGPLTE
                     AEFNNIINAVGKFYHEKKIQCFLYCYILLLAITGRRPLQLISLKAKDLIKNERGCFLN
                     VPKVKQRKCFRKEFNMVMIEPFLYDSLSMLINQNQAFVEDKFSVGISNYRGELPIFMN
                     LDKITETKRIEDFLYDLTTDFFHMKNSVMSKLLKHFPSKFDVRSERTNSYIELNARRF
                     RYTLGSRLANEGASIEVIAKALDHKSVNSSIIYIKNNPDNVYDIDKRLSAFFNPLSNI
                     LMGIEIEENKNFFIKFVSDAFFLLEDTKEDLKCLTCKKFNPWRAL"
     gene            2468..4099
                     /locus_tag="ACHJWA_004697"
     CDS             2468..4099
                     /locus_tag="ACHJWA_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO4311131.1"
                     /translation="MNNIFIFEPSNKNNPLDNVIKFIEFCKSTISNNNLTTSWESNKW
                     KGLYRFTKFNSKNNLNSKECLDDSFINFAKAYMLHVHSFNKSKTKHSTLSMLKIVEFV
                     LLKINMEANVNYCNNSIYDECIRIASEKYSKAHAFAIGKELEKLSSFLNDNRMTNSFY
                     LFWVNPIRYRITQSWTGYDSSLEGHSRLPDIKSVIAIAEIFSKRDEQLSSRDIFTTSV
                     LALLMCAPSRISEILALPADCEITECDGKGIQRYGLRFFSAKGYEGNIKWIPTLMIPV
                     AKKAISRLKELSSQARLLAAEIQKNYSNSTKGTLKENIPPDLFWYDREKKIKYSNALC
                     LLTEGQLNQNKKEMSDKLFRPTTNFFKTDIIDSDYIKGYFNVFKRHGYINEDGSPYLL
                     RTHQLRHLLNTFAQINGMDEFSIARWSGRKLISQNVSYDHRSHLQMSKAIREKKLSVC
                     VNEHRIKDIPVVDLNEFDSLSSGAVLVSKHGYCKHSYAFKPCDNYPIKNSGLDNETIS
                     NIHDKILKRTLYDKNDGNINADKWYEFHKKIKKGE"
     gene            4100..4498
                     /locus_tag="ACHJWA_004698"
     CDS             4100..4498
                     /locus_tag="ACHJWA_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311132.1"
                     /translation="MAKHINGYHEKKIVDVINTWSIDEKLTWNALCDRLVRVIGKRPS
                     RQSLSSHVRIAESFNVKKTTIKSGVIHTVKPANLKVASQRIKRLEAENEALKALNERY
                     LEQFKVWLYNAHLKQITIEELNNPLPAKYL"
BASE COUNT         1718 a          627 c          841 g         1379 t
ORIGIN      
        1 cctgatcctt gataaaaaaa gtaatctacc aacttactat caaaatctat ttcttactac
       61 agacataaga aatagaggcg caacggcctc aacgatggaa atagttgcaa ctaacttact
      121 aattttcagt aatttcttgg atggtagggg aattgatata attgagagag ttgaacttaa
      181 aaaatatctc agcgttgcag aaatagatga tctggtaaga tatgcgaaac aaagattcga
      241 cagacaaaaa atcataaata taaaatcgac aaattatagg tttattgcga agagaacctt
      301 tagttataga atacacgttt tttcacgcta ccttaagtgg ttatgtggat tagttcattc
      361 gtcaaagggg attcatgcaa agtatgaagt agacgtattt attgaaagta tcagagcaca
      421 tattccaaga aacagttcat tgaatatgaa tgaaatatct gaaaaatcgt tgaatgaaga
      481 agagatcaaa gtgcttttcc gtctattgga aattggcggg attgaaaacc ctttccataa
      541 ggaagtacag gtcaggaaca gactaatatt taccttgctc ttaaatttag gtttaagggc
      601 tggagagttg ctcaatctta aaatagatga ttttgatctc agagacaaca ctttaagtgt
      661 agtcagaaga catgattcaa aagaagatgg aagatcttat caaccattag taaagactgg
      721 agaaagggtt attccgttat ctgatgagtt agcccgtgaa atttttgatt acataagcaa
      781 tagcagagaa aaaatgacaa aaaggaaaaa gcataatttc ttatttgtgg ctcattgcac
      841 cggaaaaaac gcaggagaac ccttaagcat ttcagcttat gagaaggtga tatcaacact
      901 gaaacgagca agtccagagc tatataatct ttcaggccat aggttaaggc actcttggaa
      961 ttatatgtat tctaaaggaa tagaaggtgc tgaattagaa tataatagaa ggaaagattt
     1021 aaggaattat ttaatgggtt ggtcaaagga gtcaataatg tgtgatagat acaactataa
     1081 atatatatcg caacaagaga aggatgtcgt cctaaaggtc tataaatcag taagtaaaat
     1141 aattagtgga gtttagtatg gaaaacaaat gtattgaaag tgagcaaatc ttttttgcta
     1201 agatgaacag gtatagtttc aaactgtcag ataagaaatg gcaactggat aaagaaaact
     1261 gcgtataccc tcataaagtt gtagatagaa tgcctacaaa aatgaaactt agctacttaa
     1321 aaacattggc ttactatgcg tctgaatata gttcttttta tattcaaagt attaacaatc
     1381 tattttatga gtggtttggt gcgatgacta tcgatactat tgatgacaaa gcaatatatc
     1441 aattgaatgt ttatttaggt tcagaaagaa actacaaact aaacttaatt aaggctttca
     1501 tcattaaatg gaaaaatctc aattaccctg gggtagaagc gactgccatt agaatgctgg
     1561 agaaaataaa aatcattcca aaccaaacag gagatgcagt taaaagacaa gatccaaata
     1621 aaggaccttt aactgaagcg gaattcaata acatcattaa cgccgttgga aaattttatc
     1681 atgagaagaa aattcaatgc tttttgtatt gttatatcct tttgctggca ataacaggaa
     1741 gaaggccatt acaattaata tctctaaaag ctaaagatct cattaaaaat gagagagggt
     1801 gctttttgaa tgtaccaaaa gtaaaacaaa gaaaatgttt cagaaaagaa tttaacatgg
     1861 ttatgataga gccgttctta tatgacagct tatcaatgct aattaatcaa aatcaggcgt
     1921 ttgttgaaga taaattcagt gttgggatta gtaactatag aggcgaatta ccaatcttca
     1981 tgaatttaga taagattacg gaaacaaaaa ggattgagga ttttttatat gatttaacaa
     2041 cagatttttt ccatatgaaa aattcagtta tgtcaaaact attaaaacac tttccgtcaa
     2101 aattcgatgt taggtcagaa aggactaaca gctatataga acttaatgct agaagattca
     2161 gatatacgtt aggaagtcga ctggctaatg aaggagcctc aattgaggtg attgctaaag
     2221 cgttagatca taaatcagta aactcttcta taatatatat aaaaaataat cctgacaacg
     2281 tttatgacat cgataagaga ctaagtgcgt tttttaaccc cttatctaat atacttatgg
     2341 gcatagagat tgaagaaaac aagaactttt ttatcaagtt tgtttcagat gcatttttct
     2401 tattggaaga tacgaaagag gatttgaaat gtttaacgtg taaaaaattc aatccctgga
     2461 gagcattatg aacaatatat ttatttttga accaagcaat aaaaacaacc ctctcgataa
     2521 tgttattaag tttatcgagt tttgtaagag tactatttct aataataatt taacaacttc
     2581 atgggaaagc aataaatgga aaggtttata taggtttact aagtttaact caaaaaacaa
     2641 cctaaacagc aaggagtgct tagatgatag ctttattaat tttgctaaag catatatgtt
     2701 gcatgtgcat tcattcaata aatctaagac gaaacactca acattatcaa tgttgaagat
     2761 tgtcgaattc gttttactta aaatcaatat ggaagctaat gtaaactatt gcaacaattc
     2821 aatctatgat gaatgcataa ggatagcctc tgaaaagtat tctaaagcac atgcatttgc
     2881 tattgggaaa gaacttgaga aattgagttc atttttaaat gataatagga tgactaactc
     2941 attttattta ttttgggtaa atccaattag gtataggatt actcagtctt ggactggtta
     3001 tgattcttca ctggaaggcc attctagatt gcctgatatc aaatcagtta ttgcgattgc
     3061 tgagattttt tcaaagcggg atgaacaatt atcgtcaaga gatatattta ctacatctgt
     3121 gcttgcttta cttatgtgtg caccgagtag gatatcggaa attttagctt tgccagcgga
     3181 ttgtgaaatc acagaatgtg atggaaaggg cattcaaaga tacggtttaa gatttttttc
     3241 agcaaaaggg tatgaaggca atataaaatg gattccaact ttaatgatac ctgtagctaa
     3301 aaaggctatt agcagattaa aagaattatc aagtcaagcg aggttattgg ctgctgaaat
     3361 tcaaaagaat tactctaatt caacgaaggg aacccttaaa gaaaatatac ctcctgatct
     3421 cttttggtat gatagagaga agaaaatcaa atattctaat gcgctttgct tgttaactga
     3481 aggacagtta aatcaaaata aaaaggaaat gtcagataaa ttattcagac ctacaacgaa
     3541 tttttttaaa actgatatca ttgattctga ttatataaaa gggtatttta atgtttttaa
     3601 aagacatggt tatataaatg aagatggtag cccatatttg ctaagaacac atcaactaag
     3661 gcatcttctc aacacatttg ctcaaataaa tggtatggat gaatttagta ttgctcgctg
     3721 gtctggacgt aagcttattt ctcaaaatgt ttcttatgac cacagatcgc atcttcaaat
     3781 gtctaaagca ataagagaaa aaaagttatc agtatgtgtt aatgagcaca gaataaagga
     3841 tattccagta gtggatctta atgagtttga ctcacttagt agtggtgcag tacttgtatc
     3901 aaaacatggc tactgcaagc actcatatgc gtttaagccg tgtgataatt atccaattaa
     3961 gaactctggt ttagataacg aaacgatttc aaatatccac gataaaattt taaaaagaac
     4021 actgtatgat aaaaatgatg ggaacataaa tgctgataaa tggtatgaat tccataaaaa
     4081 aataaaaaaa ggagaataaa tggctaagca tataaatggt tatcatgaga agaagattgt
     4141 agatgttatt aacacatggt ctatagatga aaaactaacc tggaatgctt tgtgcgatag
     4201 gttagtcagg gttattggga aaagaccatc aagacaatcc ttaagtagtc atgtaaggat
     4261 tgcggaaagc ttcaatgtga aaaaaaccac aatcaaatct ggtgtaatac acactgtaaa
     4321 acctgctaat ttaaaagtgg cttctcaacg aattaaaaga cttgaagcag aaaatgaagc
     4381 tttgaaggca ttaaatgaac gctatttaga gcagttcaaa gtatggcttt ataatgctca
     4441 ccttaaacaa ataacgattg aagaacttaa taatccgtta cctgcaaaat acctttaata
     4501 aaattgatag gttttagcta ttgacatggt gaaatattat gttatttatt ttcttgtcag
     4561 gacga
//
LOCUS       DAZFAB010000202         4282 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZFAB010000202 DAZFAB010000000
VERSION     DAZFAB010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.203"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..284
                     /gene="sepL"
                     /locus_tag="ACHJWA_004699"
     CDS             <1..284
                     /gene="sepL"
                     /locus_tag="ACHJWA_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE gatekeeper SepL"
                     /protein_id="HFO4311133.1"
                     /translation="DNYADIISISIKKDKDIILNEVLSIIEHVWLTEDWLLESPSRVS
                     IVEDKHIYYFHLLKDFFTSLPDACFIDSEQRENALLMIGKVIDYKEEII"
     gene            343..921
                     /gene="espA"
                     /locus_tag="ACHJWA_004700"
     CDS             343..921
                     /gene="espA"
                     /locus_tag="ACHJWA_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     filament protein EspA"
                     /protein_id="HFO4311134.1"
                     /translation="MDTSNATSVVNVSASSSTSTIYDLGNMSKDEVVKLFEELGVFQA
                     AILMFSYMYQAQSNLSIAKFADMNEASKASTTAQKMANLVDAKIADVQSSTDKNAKAK
                     LPQDVIDYINDPRNDISVTGIRDLSGDLSAGDLQTVKAAISAKANNLTTVVNNSQLEI
                     QQMSNTLNLLTSARSDVQSLQYRTISAISLGK"
     gene            934..2058
                     /gene="espD"
                     /locus_tag="ACHJWA_004701"
     CDS             934..2058
                     /gene="espD"
                     /locus_tag="ACHJWA_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     pore-forming subunit EspD"
                     /protein_id="HFO4311135.1"
                     /translation="MLNVNNDTLSVTSGVNTASGTSGITQSETGLSLDLQLVKSMNSS
                     AGWTESSPLPTPPAGHSLVTPSAAEDVLSKLFGGISGEVTSRTEEAEPQRTSYPYLSQ
                     VNTVDPQQMMMMVTLLSLDTSAQKVSSLKNSNEIYMDGQTKALENKTQEYKKQLEEQQ
                     KAEEKSQKSKIVGQVFGWLGVALTAVAAVFNPALWAVVAIGATAMALQTAVDVMGENA
                     PQGLKTAAQVFGGISMAASILTAGVGGVSSLLSKFGNVANKIGSSVVKVVEKAAEALV
                     KNVFAKISTVAEGVTNGIRSAGTTALNNEAAQLQMLSQLAAFAVQNLTRQSESLGESA
                     KLELDKAASELQNQASYLQSVSQLMSDSARVNSRIVSGRI"
     gene            2079..3017
                     /gene="espB"
                     /locus_tag="ACHJWA_004702"
     CDS             2079..3017
                     /gene="espB"
                     /locus_tag="ACHJWA_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     pore-forming subunit EspB"
                     /protein_id="HFO4311136.1"
                     /translation="MNTIDNTQVTMVNSASESTTGASSAVAASALSIDSSLLTDGKVD
                     ICKLMLEIQKLLGKMVTLLQDYQQKQLAQSYQIQQAVFESQNKAIEEKKAAATAALVG
                     GIISSALGILGSFAAMNNAAKGAGEIAEKASSASSKAAGAASEVANKALVKATESVAD
                     VAEEASSAMQKAMATTTKAASRASGVADDVAKASDFAEDLADAAEKTSRINKLLNSVD
                     KLTNTTAFVAVTSLAEGTKTLPTTISESVKSTHEVNEQRAKSLENFQQGNLELYKQDV
                     RRTQDDITTRLRDITSAVRDLLEVQNRMGQSGRLAG"
     gene            3024..3431
                     /gene="cesD2"
                     /locus_tag="ACHJWA_004703"
     CDS             3024..3431
                     /gene="cesD2"
                     /locus_tag="ACHJWA_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LcrR family type III secretion system chaperone
                     CesD2"
                     /protein_id="HFO4311137.1"
                     /translation="MVDTFNDEVFNYYLEQKGYTIQKEFLCGSAFFIGWRIETPFFSL
                     AYRLDEQELILCSFEARNQTGLNGPVLSLTHLLEELYHHFSGIKKISAMKSKIGSDSE
                     RQKREELFNYFIRKGAVQQETEDGIWFVMNVNS"
     gene            3467..3688
                     /gene="escF"
                     /locus_tag="ACHJWA_004704"
     CDS             3467..3688
                     /gene="escF"
                     /locus_tag="ACHJWA_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE needle filament
                     protein EscF"
                     /protein_id="HFO4311138.1"
                     /translation="MNLSEITQQMGEVGKTLSDSVPELLNSTDLVNDPEKMLELQFAV
                     QQYSAYVNVESGMLKTIKDLVSTISNRSF"
     gene            3694..3972
                     /gene="escG"
                     /locus_tag="ACHJWA_004705"
     CDS             3694..3972
                     /gene="escG"
                     /locus_tag="ACHJWA_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE needle protein
                     cochaperone EscG"
                     /protein_id="HFO4311139.1"
                     /translation="MVNDISANKILVWAAVAAANHKLPKYAEAILNVFPQIIPDKKDI
                     AHLEFIILFGLNRKNDAVKALEDCMDDETSQLLYSLVHENGSGWVRGF"
     gene            4057..>4282
                     /locus_tag="ACHJWA_004706"
     CDS             4057..>4282
                     /locus_tag="ACHJWA_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspF"
                     /protein_id="HFO4311140.1"
                     /translation="MLNGISNAASTLGRQLVGIASRVSSAGGTGFSVAPQAVRLTPVK
                     VHSPFSPGSSNVNARTIFNVSSQVTSFTPSR"
BASE COUNT         1261 a          779 c         1021 g         1221 t
ORIGIN      
        1 ttgataacta tgcagatatt atttcgatct ctattaagaa agataaagat atcattttaa
       61 atgaagtgtt atcgattatt gaacatgtct ggctaacaga agactggttg ctggagagtc
      121 cttctcgggt atcgattgtc gaagataaac atatttatta ttttcattta ttgaaagact
      181 tttttacatc attaccagat gcttgcttta ttgatagtga gcagagagag aatgcattat
      241 taatgattgg taaagttatc gactataagg aggaaattat ttgatattaa ttactcaata
      301 atttttttgt tttcctgaga aaaattatca agaggtatat agatggatac atcaaatgca
      361 acatccgttg ttaatgtgag tgcgagttct tcgacatcga cgatctatga cttaggtaat
      421 atgtcgaagg atgaggtggt taagctattt gaggaactcg gtgtttttca ggctgcgatt
      481 ctcatgtttt cttatatgta tcaggcacaa agtaatctgt cgattgcaaa gtttgctgat
      541 atgaatgagg catctaaagc gtcaaccacg gcacaaaaga tggctaatct tgtggatgcc
      601 aaaattgctg atgttcagag tagcactgat aagaatgcga aagccaaact tcctcaagac
      661 gtgattgact atataaacga tccacgtaat gacataagtg taactggtat tcgtgatctt
      721 agtggtgatt taagcgctgg tgatctgcaa acagtgaagg cggctatttc agctaaagcg
      781 aataacctga caacggtagt gaataatagc cagctcgaaa ttcagcaaat gtcgaataca
      841 ttaaatctct taacgagtgc acgttctgat gtgcaatctc tacaatatag aactatttca
      901 gcaatatccc ttggtaaata accggagata actatgctta acgtaaataa cgataccctg
      961 tctgtaacgt ctggggttaa taccgcctcg ggtacttctg gtattactca atctgaaacg
     1021 ggtttatcgc tggatttaca actggttaaa tccatgaact cgtcagcagg ctggacagaa
     1081 agtagccctt taccgacgcc gccggcaggt cactcattag tgacgccctc tgctgctgag
     1141 gatgtcctta gtaaattgtt tggtggtatt agtggtgagg ttacaagtcg cactgaggag
     1201 gcagagccac agcgcacaag ctatccctat ctctctcagg tgaataccgt tgaccctcag
     1261 caaatgatga tgatggtcac tctgttatcc ctggatactt ccgcgcagaa agtctcgagt
     1321 ctgaaaaact ctaacgagat ttatatggat gggcaaacta aagcgctgga gaataaaacg
     1381 caggagtata aaaaacagct cgaagaacaa cagaaagccg aagagaaatc acaaaaaagt
     1441 aaaattgttg gccaggtctt tggttggttg ggcgtcgcat taacagccgt tgccgctgtt
     1501 tttaacccag cactctgggc tgttgttgcc attggtgcaa cagcaatggc actgcaaacg
     1561 gcagtcgatg taatggggga aaatgcccct cagggattaa agactgcagc acaggtcttt
     1621 ggcggaatat ctatggccgc aagcattctg acagccggcg ttggcggggt gtcttcactg
     1681 ttatctaaat ttggtaatgt tgctaacaaa attggctcaa gcgttgtaaa agtcgttgag
     1741 aaggcggcag aagcgctggt taaaaacgtt tttgcaaaaa tttcgacagt ggctgagggc
     1801 gttacgaacg gtattcgttc tgccgggaca actgcgttga ataatgaggc tgcgcaactc
     1861 caaatgttgt ctcagttagc tgctttcgcg gtgcaaaact taactcgaca gagtgaaagc
     1921 ttaggtgaga gtgcgaagct cgagctggat aaagcggcaa gcgagttaca aaatcaggcg
     1981 agctatttac aaagtgtttc tcaactgatg tccgattcag cacgggtaaa tagtcgtatt
     2041 gttagtggcc gaatttaatt attaaagaga attttattat gaatactatt gataatactc
     2101 aagtaacgat ggttaattcc gcttcggaga gtacgaccgg cgcttccagt gcagttgccg
     2161 catctgcttt atcaattgat tcatctctgc ttactgatgg taaggttgat atttgtaagc
     2221 tgatgctgga aattcaaaaa ctcctcggca agatggtgac tctattgcag gattaccaac
     2281 aaaaacaatt ggcgcaaagc tatcagattc agcaggccgt ttttgagagc cagaataaag
     2341 ctattgagga aaaaaaagcc gcggcaaccg ctgctttggt tggcgggatt atttcatcag
     2401 cattggggat cttaggttct tttgcagcaa tgaacaacgc ggctaaaggg gctggtgaga
     2461 ttgctgaaaa agcaagctct gcatcttcaa aggctgctgg tgcggcttct gaggttgcaa
     2521 ataaagctct ggtcaaggct acggaaagtg ttgctgatgt cgcagaggag gcatccagtg
     2581 cgatgcagaa agcgatggcc acaacaacga aagcagccag ccgtgcatct ggcgttgcag
     2641 atgatgttgc gaaagcctct gactttgctg aagatcttgc agacgccgcc gagaagacaa
     2701 gcagaatcaa taagttgttg aattccgtag ataaactgac caataccaca gcatttgttg
     2761 ccgtgaccag tcttgctgaa ggtacgaaaa cgttgccaac aacaatatct gagtccgtca
     2821 aatcgactca tgaggttaat gaacaacgtg cgaagtcgct ggaaaacttc cagcagggga
     2881 atctggagct gtataaacaa gacgttcgca gaacgcagga tgatatcacg actcgtctgc
     2941 gtgatataac gtccgctgtc cgcgatctcc ttgaggtcca gaatcgtatg gggcaatcgg
     3001 gtcgcttagc tgggtaattg atcatggtcg atacgtttaa tgatgaagtg tttaattact
     3061 atcttgaaca aaaagggtac acaatacaga aggagtttct ttgtggcagt gcctttttta
     3121 tcggatggcg gattgagaca cctttttttt cattagcgta cagactggat gaacaagaac
     3181 tgattttgtg ctcttttgaa gcacgtaacc aaacagggct taacggccct gttttatcgc
     3241 tgactcattt gctcgaagag ttgtatcacc atttttcggg tattaagaaa atcagcgcga
     3301 tgaaatctaa gattggttca gattcagaac gtcaaaagcg cgaagagttg tttaattact
     3361 tcatcagaaa gggtgccgtg cagcaagaaa cagaagacgg aatttggttc gtaatgaacg
     3421 taaatagtta aaagttttta ttttattaac ttctgaggga aatttaatga atttatctga
     3481 aattactcaa caaatgggtg aagtaggtaa aacgctgagc gattctgtgc cagagttact
     3541 taatagcacc gatttggtta atgaccctga aaaaatgtta gagttgcagt ttgcggttca
     3601 gcaatattct gcttatgtta acgtagaaag tggaatgttg aaaacgataa aagatctggt
     3661 ctcaaccatt tctaaccgta gtttttaatt ttaatggtta atgatatttc tgctaataag
     3721 atactggtgt gggcggctgt agctgcggca aaccataagc ttcccaaata tgcggaagct
     3781 atcctgaatg tattcccaca aattatacct gataaaaaag atatcgcaca tttagaattt
     3841 attatcttat ttggattaaa tagaaaaaat gatgcggtaa aggctttgga ggactgtatg
     3901 gatgatgaga cgagtcagtt attgtacagt ttggtccatg agaatggtag tggctgggta
     3961 cgaggatttt aatttatatt gatttaaaaa tcattttatt attcgttttg atatatatga
     4021 gagttagcca agcttagata taaagaggca taaattatgc ttaatggaat tagtaacgct
     4081 gcttctacac tagggcggca gcttgtaggt atcgcaagtc gagtgagctc tgcgggggga
     4141 actggatttt ctgtagcccc tcaggccgtg cgtcttactc cggtgaaagt tcattcccct
     4201 ttttctccag gctcgtcgaa tgttaatgcg agaacgattt ttaatgtgag cagccaggtg
     4261 acttcattta ctccctctcg tc
//
LOCUS       DAZFAB010000203         4271 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZFAB010000203 DAZFAB010000000
VERSION     DAZFAB010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.204"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(32..2008)
                     /gene="speA"
                     /locus_tag="ACHJWA_004707"
     CDS             complement(32..2008)
                     /gene="speA"
                     /locus_tag="ACHJWA_004707"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFO4311141.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2017..2148)
                     /gene="yqgB"
                     /locus_tag="ACHJWA_004708"
     CDS             complement(2017..2148)
                     /gene="yqgB"
                     /locus_tag="ACHJWA_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFO4311142.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            complement(2242..2385)
                     /gene="yqgG"
                     /locus_tag="ACHJWA_004709"
     CDS             complement(2242..2385)
                     /gene="yqgG"
                     /locus_tag="ACHJWA_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFO4311143.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(2496..2747)
                     /gene="yqgD"
                     /locus_tag="ACHJWA_004710"
     CDS             complement(2496..2747)
                     /gene="yqgD"
                     /locus_tag="ACHJWA_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFO4311144.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            2803..3957
                     /gene="metK"
                     /locus_tag="ACHJWA_004711"
     CDS             2803..3957
                     /gene="metK"
                     /locus_tag="ACHJWA_004711"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFO4311145.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
BASE COUNT         1095 a         1153 c         1020 g         1003 t
ORIGIN      
        1 ataaaccgac ccgccagcag aataatctta attattcatc ttcaagatag gtataaccgt
       61 acaaacctgc ctcgaactct tcaaggaact gttgttgcag ttcagcatca agatcggttt
      121 tcttcacttg atcacggaac tgagttaaca gcgttttcgg atcgagctgt acatattgca
      181 gcatgtccgc cacggtatcg ccttcgtcag acagttccac ttctacacta ccgtcaggga
      241 agacgaacac gtcaaccgct tcggtatcac cgaacaggtt gtgcatgttg ccgaggatct
      301 cctgatatgc gccgaccata aaaaaaccga gcatcggcgg attctctgga tcgtactccg
      361 gcataggcat tgtcgtggca ataccatcac catcgatata gtggtcgata gcaccgtcag
      421 agtcacaggt aatatccagc agcacagcgc ggcgttccgg cacttgatcc agcccttcca
      481 gcggcagaac cgggaacaac tggtcgatcc cccacgcatc cggcatcgac tggaacagcg
      541 agaagttgac gtacattttg tccgccatac gttcctgcag ctcgtcgata atcggacgat
      601 gagcacggtt ttgcggatcc agctgctttt gcacttcatg gcacatgctc aaataaagct
      661 gctcagccca tgcacgttct tgcaggctaa aggtgccgga agagtagccg atatgaatgt
      721 cgtgcagatc catctgactg tcgtgtaacc attcacgcag agaacggcga gttcccggtt
      781 cgtgcatctc ctgccaggtt tcccacatgc tttgcagcgc gcgcggcgca tcttctgcag
      841 gcgcggtcgg caccgtgtat tcgttacgtt ccacgccgat gatattagac accagcacgg
      901 tgtgatgcgc agtcactgca cgacccgatt cggtgattac cgtcggatgc ggcagaccgt
      961 tttcttcaca cgcatcacca atcgcccaga tgatgttgtt ggcatattca ttgaggccgt
     1021 agttcaccga acagtcggac tgcgaacgag taccttcata atccacgccc agaccgccgc
     1081 cgacgtcgaa gcactgaata ttgacgccca gcttgtgcag ctcaacatag aaacgcgcgg
     1141 attcacgaac gcctgtcgcg atatcgcgaa tattcgccat ctgcgaaccg aggtggaagt
     1201 gcagtagttg caggctgtcg agacgcccgg cttcacgtag ggtttcaacc agttgcagta
     1261 cctgagtcgc cgccaggccg aacttcgatt tttccccgcc ggaggactgc catttaccgg
     1321 agccctgcga agccagacgt gcacgcacgc ccagacgagg aacgacattc agacgttctg
     1381 cttcatccag cacaatggcg atttctgaca tcttctcaat gaccagatag accttgtgcc
     1441 ccatcttctc gccaattaat gccaggcgga tatattcgcg gtctttataa ccattgcaga
     1501 cgatgacgct acgggtcatg ccagcatgtg ccagcactgc catcaactcg gctttggaac
     1561 cggcttccag acccagcggt tcgcccgaat gaatcaggga ctcaatcacg cgacggtgct
     1621 ggttaacttt gatcggataa acaaggaagt aatcgccgtt atagccgtag gattcccgcg
     1681 cacgtttgaa cgcggcgtta atggaacgca aacggtgctg caggatctgt gggaaacaga
     1741 acagtgcagg cagacgctga ccctgtgctt cacgagtttt cactaactgc gcgagatcga
     1801 cgcgagcttc cgggacgtcc gggtccgggc acacgctgat gtggcccagc tcgttaacgt
     1861 catagtagtt attgccccac caggcaatat tgtaagtacg cagcatcttg ctggcttcct
     1921 gggagctcat tgcaacctcc tgcatggagc gtagtacacc gtgttcgccc gctgacgaag
     1981 gcaaacccat agacatgtcg tcagacatag cgaacctcaa attattttat taagtgtaaa
     2041 acagttaacg actatcgcag cctggaactg cgataacaac ccataagcac atggattttc
     2101 cagcagtgaa tgctgacgct ccaactgcgc aaccggtttc tttttcataa cattattaag
     2161 cacataaccg aacgtaagtg tgacagttcg gcgaaaccac gagaaaactc ttgtttttac
     2221 aagagcgccc ttgttcagtc ctcagtaact gtaaccagct cttgaatcct gagaagcgcc
     2281 gagatgggta taacatcggc aggtatgcaa agcagagatg cagagtgcgg ggaacgaatc
     2341 ttcaccagaa cggtgagaca ggttaagcag cagacaacgg ttcattattt cgtatcacct
     2401 ccacggccgc ctgttaagac gaacccacaa gccaaaactc tgatttcaac ccggctggaa
     2461 gtggcaacac gaaagaaacg tcgtgtgctt tttatttaag ccgcgcgccg cgttttatac
     2521 cccacaatgg cagaaaattg caaaagataa atacgcagaa tgccggcatt gtcaggaaaa
     2581 atttccagcc acgtttttaa cagaatgaga cacgattcaa aaaaaagtgg aaatcgggtg
     2641 aagaattgac ctaaaatagc catccagatg ttaatccatc cataccgatt aacactcaga
     2701 ctgccagtgt ttttaacctg cagagtcgtg gtaggatccg ctaccacaga aaatccacac
     2761 aacagtttga gctaaccaaa ttctctttag gtgatattaa atatggcaaa acaccttttt
     2821 acgtccgagt ccgtctctga agggcatcct gacaaaattg ctgaccaaat ctctgatgcc
     2881 gttttagacg cgatcctcga acaggatccg aaagcacgcg ttgcttgcga aacctacgta
     2941 aaaaccggca tggttttagt tggcggcgaa atcaccacca gcgcctgggt agacatcgaa
     3001 gagatcaccc gtaacaccgt tcgcgaaatt ggctatgtgc attccgacat gggctttgac
     3061 gctaactcct gtgcagttct gagcgctatc ggcaaacagt ctcctgacat caaccagggc
     3121 gttgaccgtg ccgatccgct ggaacagggc gcgggtgacc agggtctgat gtttggctac
     3181 gcaactaatg aaaccgacgt gctgatgcca gcacctatca cctatgcaca ccgtctggta
     3241 cagcgtcagg ctgaagtgcg taaaaacggc actctgccgt ggctgcgccc ggacgcgaaa
     3301 agccaggtga ctttccagta tgacgacggc aaaatcgttg gtatcgatgc tgtcgtgctt
     3361 tccactcagc actctgaaga gatcgaccag aaatcgctgc aagaagcggt aatggaagag
     3421 atcatcaagc cgattctgcc cgctgaatgg ctgacttctg ccaccaaatt cttcatcaac
     3481 ccgaccggtc gttttgttat cggtggcccg atgggtgact gcggtctgac tggtcgtaaa
     3541 attatcgttg atacctacgg cggcatggcg cgtcacggtg gcggtgcatt ctctggtaaa
     3601 gatccatcaa aagtggaccg ttccgcagcc tacgcagcac gttatgtcgc gaaaaacatc
     3661 gttgctgctg gcctggccga tcgttgtgaa attcaggttt cctacgcaat cggcgtggct
     3721 gaaccgactt ccatcatggt agaaactttc ggtactgaga aagtgccttc tgaacaactg
     3781 actctgctgg tacgtgagtt cttcgacctg cgcccatacg gtctgattca gatgctggat
     3841 ctgctgcacc cgatctacaa agaaaccgca gcatacggtc actttggtcg tgaacatttc
     3901 ccgtgggaaa aaaccgacaa agcgcagctg ctgcgcgatg ctgccggtct gaagtaatct
     3961 ttcttcacct gctttcaaag gccagcctcg cgctggcctt tttcttttac ctcaccgcgg
     4021 tggataggcg ttcacgccgc atccggcaaa aaaaccgcct gcacaataac atcattcttc
     4081 ctgatcacgt ttcaccgcaa attatcatca caactgaaac cgattacacc aaccacaaca
     4141 gacaaagatt tgtaatattt ccatattatt attcggtttt cacagttgtt acatttcttt
     4201 tcagtaaagt cttaattgca gataacagcg tttaatctat gatgatataa ctcaattatt
     4261 ttcatgcact t
//
LOCUS       DAZFAB010000204         4231 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZFAB010000204 DAZFAB010000000
VERSION     DAZFAB010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4231
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.205"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            36..398
                     /gene="rusA"
                     /locus_tag="ACHJWA_004712"
     CDS             36..398
                     /gene="rusA"
                     /locus_tag="ACHJWA_004712"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFO4311146.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHVSAEGQAYRDNVARIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            395..535
                     /locus_tag="ACHJWA_004713"
     CDS             395..535
                     /locus_tag="ACHJWA_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFO4311147.1"
                     /translation="MMFEFNMAELLRHRWGRLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            621..1055
                     /locus_tag="ACHJWA_004714"
     CDS             621..1055
                     /locus_tag="ACHJWA_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO4311148.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLIPEKVKSRPQ
                     CCDDDAMVICGCIARLYRNNRDLHDLLVDYYVLGETFMALARKHGCSDTCIGKRLHKA
                     EGIVEGMLMMLGVRLEMDRYVERELPGGRTSVFYQRKNSLRS"
     gene            1562..2509
                     /gene="stxA1a"
                     /locus_tag="ACHJWA_004715"
     CDS             1562..2509
                     /gene="stxA1a"
                     /locus_tag="ACHJWA_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit A"
                     /protein_id="HFO4311149.1"
                     /translation="MKIIIFRVLTFFFVIFSVNVVAKEFTLDFSTAKTYVDSLNVIRS
                     AIGTPLQTISSGGTSLLMIDSGTGDNLFAVDVRGIDPEEGRFNNLRLIVERNNLYVTG
                     FVNRTNNVFYRFADFSHVTFPGTTAVTLSGDSSYTTLQRVAGISRTGMQINRHSLTTS
                     YLDLMSHSGTSLTQSVARAMLRFVTVTAEALRFRQIQRGFRTTLDDLSGRSYVMTAED
                     VDLTLNWGRLSSVLPDYHGQDSVRVGRISFGSINAILGSVALILNCHHHASRVARMAS
                     DEFPSMCPADGRVRGITHNKILWDSSTLGAILMRRTISS"
     gene            2519..2788
                     /gene="stxB1a"
                     /locus_tag="ACHJWA_004716"
     CDS             2519..2788
                     /gene="stxB1a"
                     /locus_tag="ACHJWA_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit B"
                     /protein_id="HFO4311150.1"
                     /translation="MKKTLLIAASLSFFSASALATPDCVTGKVEYTKYNDDDTFTVKV
                     GDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR"
     gene            3288..>4231
                     /locus_tag="ACHJWA_004717"
     CDS             3288..>4231
                     /locus_tag="ACHJWA_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO4311151.1"
                     /translation="MTFKHYDVVRAASPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVLVKPSDGEGTVISATRDTEYYFVVVLAGQSNSMAYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPGGAACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGT
                     VGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGADGTYSDASGASENSTRWGVDK
                     PLYKDLIGRTKAALKKNPKNVLFAVVWMQGEFDFGGTPVNHAAQFGALVDKFRADLAD
                     MAGQCVGGSAGGVPWICGDTTYFWKQKNESSYQTVYGS"
BASE COUNT         1076 a          863 c         1137 g         1155 t
ORIGIN      
        1 caggttatct ggctgaaaga gggggtaatc aaggcgtgaa tacctacagc atcacattac
       61 cctggcctcc gagcaataat cgctattacc gccataatcg cgggcgcacg cacgtcagcg
      121 cagaggggca ggcataccgc gataacgtcg cccgaatcat taaaaacgca atgctggata
      181 tcggcctggc tatgcctgtg aaaatccgca ttgagtgcca catgccggat cgccgtcgcc
      241 gtgacctgga taatctgcaa aaagccgctt ttgacgcact cactaaagca ggtttctggc
      301 tggatgatgc tcaggtcgtt gattaccgcg ttgtgaagat gcctgttacc aaaggtggga
      361 ggctggaact gaccatcacc gaaatgggga atgaatgatg tttgagttta atatggcaga
      421 acttcttcgc caccgctggg ggcgtctgcg cttatatcgt ttccccggtt ctgttttgac
      481 cgattaccga atactgaaga attacgccaa aaccctgaca ggagcaggag tatgaagtca
      541 gagataacaa tcaactaata ctgttttgtt gatttttgct tgtaattggc gttctggtct
      601 gatttttgtg gagtaagttg atgcgtgata ttcagatggt tcttgagcgt tggggagcgt
      661 gggcggctaa taatcatgaa gatgtgacct ggtcgtccat tgccgccggt tttaagggac
      721 tgatccccga aaaagtaaaa tcacgcccgc agtgctgtga cgatgatgcg atggtgatat
      781 gcgggtgtat agcccgcctt taccggaaca atcgcgatct gcatgacttg ctggttgatt
      841 attacgtgtt gggggagacg ttcatggcgc tggcacggaa acatgggtgc tctgacacct
      901 gtataggtaa acgccttcac aaagcggagg ggattgttga aggcatgctg atgatgctgg
      961 gagtgaggct tgagatggat cggtatgttg agcgtgaatt gccgggaggg agaacctctg
     1021 tattttatca gcgaaaaaat agtttacgat cgtaaaaatc tgcatatcat gataagagtg
     1081 gttacattgc cacgctgctt aacccgccga tgcgcgggtt tttttgtacc cagaatcctg
     1141 tgagctatac ggaaagtaca cagaaaggaa ggtgcgacca caattaataa caaaatctta
     1201 aaaattgcac atggcactat tagttttcta aatattgtgt attttttgta ttgcaggatg
     1261 accctgtaac gaagtttgcg taacagcatt ttgctctacg agtttgccag cctcccccag
     1321 tggctggctt ttttatgtcc gtaacatcct gtgtatcaat aaatgttgtt atctacgtac
     1381 gtcaagtagt cgcatgagat ctgaccagat atgttaaggt tgcagctctc tttgaatatg
     1441 attatcattt tcattacgtt attgttacgt ttatccggtg cgccgtaaaa cgccgtcctt
     1501 cagggcgtgg aggatgtcaa gaatatagtt atcgtatggt gctcaaggag tattgtgtaa
     1561 tatgaaaata attattttta gagtgctaac ttttttcttt gttatctttt cagttaatgt
     1621 ggtggcgaag gaatttacct tagacttctc gactgcaaag acgtatgtag attcgctgaa
     1681 tgtcattcgc tctgcaatag gtactccatt acagactatt tcatcaggag gtacgtcttt
     1741 actgatgatt gatagtggca caggggataa tttgtttgca gttgatgtca gagggataga
     1801 tccagaggaa gggcggttta ataatctacg gcttattgtt gaacgaaata atttatatgt
     1861 gacaggattt gttaacagga caaataatgt tttttatcgc tttgctgatt tttcacatgt
     1921 tacctttcca ggtacaacag cggttacatt gtctggtgac agtagctata ccacgttaca
     1981 gcgtgttgca gggatcagtc gtacggggat gcagataaat cgccattcgt tgactacttc
     2041 ttatctggat ttaatgtcgc atagtggaac ctcactgacg cagtctgtgg caagagcgat
     2101 gttacggttt gttactgtga cagctgaagc tttacgtttt cggcaaatac agaggggatt
     2161 tcgtacaaca ctggatgatc tcagtgggcg ttcttatgta atgactgctg aagatgttga
     2221 tcttacattg aactggggaa ggttgagtag cgtcctgcct gactatcatg gacaagactc
     2281 tgttcgtgta ggaagaattt cttttggaag cattaatgca attctgggaa gcgtggcatt
     2341 aatactgaat tgtcatcatc atgcatcgcg agttgccaga atggcatctg atgagtttcc
     2401 ttctatgtgt ccggcagatg gaagagtccg tgggattacg cacaataaaa tattgtggga
     2461 ttcatccact ctgggggcaa ttctgatgcg cagaactatt agcagttgag ggggtaaaat
     2521 gaaaaaaaca ttattaatag ctgcatcgct ttcatttttt tcagcaagtg cgctggcgac
     2581 gcctgattgt gtaactggaa aggtggagta tacaaaatat aatgatgacg atacctttac
     2641 agttaaagtg ggtgataaag aattatttac caacagatgg aatcttcagt ctcttcttct
     2701 cagtgcgcaa attacgggga tgactgtaac cattaaaact aatgcctgtc ataatggagg
     2761 gggattcagc gaagttattt ttcgttgact cagaatagct cagtgaaaat agcaggcgga
     2821 gattcataaa tgttaaatac atctcaattc agtcagttgt tgccggtctg ataatagatg
     2881 tgttagaaaa tttctgcatg gtgaatcccc ctgtgcggag gggcgactgg tgaacggtat
     2941 gatctctttg atgatcgtaa gcgagaatac gcgggtttgg tggcaccagg ccgaactcac
     3001 cgggaggcac ccggcatcat gctgtataca gagattaggc atatatccag gctcctcatc
     3061 gcaggagcct ttttacatgc aaaaaaaagc ccgagtgggt tcgggcaaca gcatgagata
     3121 cttgcattgt catttttatc gtgcggattt taaccaggat tcataaggct gcgcaactgc
     3181 gcggcctttt tcgtatttcg ggctgtagtc cccgtgtgtc attcaggctt ccggactaca
     3241 gcccactcca tatctgattt aatacactat cccggccggg aggaataatg acatttaaac
     3301 attatgatgt tgtcagggcg gcgtcgccgt cagaccttgc ggaaaagctg acacacaaac
     3361 tgaaagaggg ctggcagccg tttggtagtc cggtggccat cacgccttat actctgatgc
     3421 aggccattgc ggcggaaggt gatgtcacca cacctgtgtt ggtgaagccg tcggatggag
     3481 aaggcacagt aatcagcgcc accagagaca cggagtatta ctttgttgtg gttctggcgg
     3541 ggcagtcaaa cagcatggca tatggtgaag gccttccgct gccggagaca tatgaccgtc
     3601 cggacccgcg tattaagcag ctggcgcgcc gcagtacggt gacaccgggc ggtgcagcat
     3661 gcaaatataa cgacatcatt ccggcggacc attgtctgca tgatgtgcag gacatgagcc
     3721 gccttaacca tccgaaagcg gacctgtcaa aggggcagta cggaaccgtg gggcaagggc
     3781 tgcatatcgc caaaaaactg ctgccgttta taccggcgaa tgcgggcatt ctgctggttc
     3841 cgtgctgtcg tggtggttca gcgttcacca ccggagccga tggcacatac agtgacgcga
     3901 gtggtgcctc ggagaattca acccgctggg gtgtggacaa gccgctgtat aaggacctta
     3961 tcggtcgaac aaaagcagca ctgaagaaga acccgaaaaa tgtgctgttt gccgtggtgt
     4021 ggatgcaggg ggaatttgat tttggcggta cgccggtaaa tcacgcagca cagtttggtg
     4081 cgctggttga taaattccgt gcagacctgg cggatatggc aggtcagtgc gtcggtggct
     4141 ctgctggcgg tgttccctgg atatgtggag atacgacgta tttctggaag cagaagaacg
     4201 aatcctcgta ccagacggtg tacggcagct a
//
LOCUS       DAZFAB010000205         4227 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZFAB010000205 DAZFAB010000000
VERSION     DAZFAB010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4227
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.206"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            246..854
                     /locus_tag="ACHJWA_004718"
     CDS             246..854
                     /locus_tag="ACHJWA_004718"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFO4311152.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVTQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            952..2343
                     /gene="selA"
                     /locus_tag="ACHJWA_004719"
     CDS             952..2343
                     /gene="selA"
                     /locus_tag="ACHJWA_004719"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFO4311153.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2340..4184
                     /gene="selB"
                     /locus_tag="ACHJWA_004720"
     CDS             2340..4184
                     /gene="selB"
                     /locus_tag="ACHJWA_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFO4311154.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALNIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDPPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQCLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
BASE COUNT          971 a         1075 c         1214 g          967 t
ORIGIN      
        1 gccggttttc cgctcatctg ctcatccttg ttttatcggg ttatttgtct gcttccaggc
       61 aaaaccctac agatttataa gaaagcctta agtgagatat ccatcagata gaaacaatta
      121 ggaaaatatt tatatcacca tgataaatat gtgatttatt tggaagtaac aaaccatcaa
      181 cattttctgc cctttttccc atcaccaggc catactgata tttttcctcg cacaaaggac
      241 tcggcatgaa actcgtcggt agctacacca gcccgtttgt acgcaaactt tctattctgt
      301 tgttagaaaa gggcataact ttcgaattta ttaatgaact gccctataac gcggacaacg
      361 gcgtgacgca atttaacccg ttaggaaaag tgccggtgct ggtgaccgaa gagggcgaat
      421 gctggtttga ttcgccgatc atcgctgaat atattgaatt aatgaatgtc gctccggcga
      481 tgttgccgcg cgatccgctg gagtcgttgc gggtgcgcaa aattgaggca ctggcggatg
      541 gcattatgga tgccgggctg gtatcggtgc gtgaacaggc gcgtccagcg gcgcagcagt
      601 ctgaagatga attgttacgc cagcgggaga aaatcaaccg cagtctggat gtgctggaag
      661 gatatctggt cgatggcaca ctcaaaaccg atacggtcaa tctggcgact atcgccattg
      721 cctgtgccgt cggatatctc aatttccgcc gcgttgcgcc aggttggtgt gtcgaccgcc
      781 cacacttagt caaactggtc gaaaacctgt ttagccgcga aagttttgct cgcaccgaac
      841 cgccaaaggc ttgatgcgcg atatgtcctc ctgacccatc tcacgttaca atccgtggtt
      901 atgttaaacg cccttctccg tgtgagaggg ccttgatcag ccaggtttcc tatgacaacc
      961 gaaacgcgtt ccctctatag tcaacttccg gctattgatc gcttattgcg cgatagctcc
     1021 ttcctttctt tgcgtgatac ttatggtcac acccgcgtgg tggaattgtt gcgtcagatg
     1081 ctcgacgaag cgcgagaagt gattcgtggc agccagacgc tgcctgcgtg gtgtgaaaac
     1141 tgggcgcaag aagtcgatgc ccggttgacg aaagaagcgc agagcgcgct gcgtccggtg
     1201 atcaacctga cgggaaccgt gctgcatacc aaccttgggc gagctttaca ggcggaagcc
     1261 gcggtggaag ccgttgcgca ggctatgcgt tcgccagtga ccctcgagta cgatctggac
     1321 gacgccggac gcggacatcg cgatcgggcg ctggcgcagc tgctgtgccg tattacgggg
     1381 gcggaagatg cctgtatcgt caataacaat gcggcggcgg tgttattgat gttggcggcc
     1441 actgccagcg gaaaagaggt ggtggtatct cgcggcgaac tggtggagat tggcggcgcg
     1501 tttcgtattc ccgatgttat gcgtcaggca ggctgcaccc tacacgaagt agggaccacc
     1561 aaccgcacgc acgcgaatga ttatcgtcag gcggtgaatg aaaataccgc actgttgatg
     1621 aaagtacata ccagtaacta cagcattcag gggttcacca aagcgataga tgaagcggaa
     1681 ctggtggcgc tcggcaaaga gctggatgtt cccgtagtga ctgatttagg cagtggctcg
     1741 ctggtcgatc ttagccagta cggtttgccg aaagagccaa tgccgcagga gttgattgcg
     1801 gcgggcgtca gtctggtgag cttctccggc gacaagttgt taggcgggcc gcaggcaggg
     1861 attatcgttg gtaaaaaaga gatgatcgcc cgcctgcaaa gccacccgct gaagcgtgca
     1921 ttacgcgcgg ataaaatgac cctcgcggcg ctggaagcca cgttgcgtct ttatttacac
     1981 cctgaagctc tgagtgaaaa attaccgacc ctgcgcctgc ttacccgcag cgcagaggtc
     2041 attcaaatcc aggcacaacg tttacaggct ccccttgccg cacattacgg cgcggagttt
     2101 gcggtacagg ttatgccatg tctttcgcag attggcagtg gttcgctgcc ggttgatcgc
     2161 ctgccgagcg cggcattaac gtttacaccc catgatggac gcggtagcca ccttgagtca
     2221 ttagccgccc gctggcgtga attgccagtg ccggtgattg gtcgtattta tgacggacga
     2281 ttgtggctgg atttacgctg ccttgaagat gagcaacggt ttttggagat gttgttgaaa
     2341 tgattattgc gactgccgga cacgttgacc acggcaaaac aaccttattg caggcgatta
     2401 ctggcgtaaa tgctgaccgt ctgccggaag aaaaaaagcg cggcatgacc atcgatctcg
     2461 gctatgccta ctggccgcag ccggatggtc gcgtgcctgg ttttatcgac gtacccggtc
     2521 atgaaaagtt tctttccaac atgctggcgg gcgttggtgg tatcgatcac gcgctgttgg
     2581 tggtggcgtg cgatgacggc gtgatggcac agacccgtga gcatctggcg attttgcagc
     2641 tgaccggtaa cccgatgctg acagtggcgc tgaccaaagc cgatcgcgta gacgaagcgc
     2701 gtgttgatga ggttgaacgc caggtaaagg aggttctgcg ggaatacggt tttgctgagg
     2761 caaaactgtt tatcaccgca gcaaccgaag gtcggggaat tgatgccctg cgcgagcatc
     2821 tgcttcagtt gccggaacgc gagcacgcca gccaacatag tttccgcctc gcgattgacc
     2881 gcgcatttac cgtaaaaggt gccgggctgg tcgtcaccgg tacggcgtta agcggggaag
     2941 tgaaggtagg cgattcactc tggctgactg gtgtaaataa accgatgcgt gtacgtgcgc
     3001 tgcatgcgca aaaccagcca acagaaaccg ccaatgccgg gcagcgtatc gcgcttaaca
     3061 tcgcgggtga tgcggaaaaa gagcagatta accgtggcga ctggctgctt gccgatgtgc
     3121 cgccagagcc gttcacacgg gtgattgtcg agcttcaaac ccatacaccg ctgacccagt
     3181 ggcagccgct gcatattcac cacgccgcca gccacgtcac gggacgcgtt tcactgctgg
     3241 aagataacct tgctgaactg gtcttcgacc ccccgttatg gctggcagat aacgaccgcc
     3301 tggtattgcg cgatatctct gcccgcaaca cgctggccgg agcgcgcgtc gtgatgctta
     3361 acccgccgcg tcgcggtaaa cgtaaaccag agtatctgca atgcctggct tccctcgcca
     3421 gggcacagaa tgatagcgaa gcgttagccg ttcacctgca acgcggcgcg gttaatcttc
     3481 ctgatttcgc ctgggcgcgc cagctcaacg gcgaagggat gcgcgaactg ctgcaacagc
     3541 ctggctatat tcaggctggt tatagcttgt tgaatgcgcc ggttgccgct cgctggcaac
     3601 ggaaaattct cgacacatta gcgacttatc atgagcaaca tcgcgatgaa cctgggcctg
     3661 ggcgcgaacg tctgcgacgt atggcgttgc caatggaaga tgaagcgcta gtactgttgc
     3721 tgattgaaaa gatgcgcgaa agcggcgaca tcctcagtca tcacggctgg ctgcatctgc
     3781 cggatcacaa agcgggcttc agcgaagagc agcaggccat ctggcaaaaa gcagagccgc
     3841 tgtttggtga cgagccgtgg tgggtgcgtg atctggcaaa agagacgggt accgacgagc
     3901 aggcaatgcg cctgacgcta cgccaggcgg cgcagcaagg aataattacc gcgatcgtca
     3961 aagatcgtta ttaccgtaac gatcggattg tcgaatttgc caatatgatc cgcgatctcg
     4021 atcaggagtg tggttcaacc tgcgcggcgg atttccgcga tcgcttaggc gtaggccgaa
     4081 agctggcaat tcagattctg gaatattttg accgcattgg ctttacgcgt cgtcgtggaa
     4141 atgatcattt attgcgcgac gcattattat ttccggaaaa ataaggaaat gattaattaa
     4201 tttttaaaat aaattaatac aaatttc
//
LOCUS       DAZFAB010000206         4217 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZFAB010000206 DAZFAB010000000
VERSION     DAZFAB010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.207"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..209)
                     /locus_tag="ACHJWA_004721"
     CDS             complement(<1..209)
                     /locus_tag="ACHJWA_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO4311155.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="ACHJWA_004722"
     tRNA            complement(324..399)
                     /locus_tag="ACHJWA_004722"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="ACHJWA_004723"
     tRNA            complement(406..480)
                     /locus_tag="ACHJWA_004723"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="ACHJWA_004724"
     tRNA            complement(597..681)
                     /locus_tag="ACHJWA_004724"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="ACHJWA_004725"
     tRNA            complement(690..765)
                     /locus_tag="ACHJWA_004725"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="ACHJWA_004726"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="ACHJWA_004726"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFO4311156.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHJWA_004727"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHJWA_004727"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFO4311157.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVTVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHJWA_004728"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHJWA_004728"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFO4311158.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFDAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLSQFPTAPNYPQADGSVKLAAGWLIDQCQLKG
                     MQMGGVAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1083 a         1047 c          944 g         1143 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctggaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagcgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc gacacgatga agaaacagcc gaatggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctgggagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctctg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg tagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcactat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaagtc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttctgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagaca tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagctatta agactgccat gacgttgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgcga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgcg gtagaagagg tcagactacg caaataattt gcaggggagc taatgctccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagcaataa agcgccttgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccaa cttttcccag
     2281 cgcgaaagat aaggtgccaa tccttcttgt tcgaagagtt ccagcgccgc gcgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcgagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacgacgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccggtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agcttgcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gaccccggcg accacggcca gcctgctggt attctgcaac gcaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggtactgat tcgtggagtc aatcactggc
     2821 agcacagtta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 ataggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcctaacgt ttcacccaac
     3001 tgctcacctg agtgaaattc gccattcgct aacagcgcga ttaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc aaccccaccc atttgcatcc cttttagctg gcactgatcg ataagccaac
     3301 ctgctgccag ttttactgaa ccatccgcct gggggtaatt tggtgctgtt ggaaattgtg
     3361 acagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcatcaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcgg tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgccct tctgcggttg catgctgcca ggcattgagt agttgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagtt atgacgacag ggagagtttg tagaaacgca aaaaggccca
     4201 gtcttccgac cgggcct
//
LOCUS       DAZFAB010000207         4158 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZFAB010000207 DAZFAB010000000
VERSION     DAZFAB010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4158
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.208"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..159
                     /locus_tag="ACHJWA_004729"
     CDS             <1..159
                     /locus_tag="ACHJWA_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein NleE"
                     /protein_id="HFO4311159.1"
                     /translation="DIMQELNYELPELKAVKSEMIIAREMGEIFSYMPGEIDSYMKYI
                     NNKLSKIE"
     gene            537..1514
                     /locus_tag="ACHJWA_004730"
                     /pseudo
     CDS             537..1514
                     /locus_tag="ACHJWA_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041206064.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            2034..>4158
                     /locus_tag="ACHJWA_004731"
     CDS             2034..>4158
                     /locus_tag="ACHJWA_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TcdA/TcdB catalytic glycosyltransferase
                     domain-containing protein"
                     /protein_id="HFO4311160.1"
                     /translation="MRLPEKVLFPPVTSGLSGQEKQKKPKSITGFQENYQRNIRPIKT
                     ASEARLRFFDKMVSKENSLEDVVSLGEMIQKEIYGHEQRTFSPVHHTGNWKSSLLHNA
                     LLGLANVYNGLRETEYPNTFNRDGIKSTNSFRDNLLTKTRTPRDNFEEGIKHPEHATI
                     PYDNDNESNKLLKAGKIAGNNNELLMEIKKESQSDHQIPLSDKFLKRKKRSPVAEDKV
                     QNSLTPENFVQKISLSDELKTKYANEILEIKRIMGEYNLLPDKNSRNGLKLLQKQADL
                     LKIIMEDTSVTENTFKNIEMAIADIKREYYSHTVDIEKNIHAIWVAGSPPESISDYIK
                     TFLKTYKEFTYYLWVDEKAFGAAKFTSVLKQIAFDLACRTIQQNTPQKNIDFINLYNE
                     IRKKYNNNPSGQQEYLNKLRELYATYQKISTPLKHMFNSFFLENMIKLQDNFFNYCIV
                     KGVTEINDELRINYLKNVIKLSDDDIGNYQKTINDNKDRVKKLILDLQKQFGENRISI
                     KDVNSLTSLSKSENNHNYQTEMLLRWNYPAASDLLRMYILKEHGGIYTDTDMMPAYSK
                     QVIFKIMMQTNGDNRFLEDLKLRRAISDGVLRYVNNQNIDEVNYNEISDADKNIIKKI
                     LTEISKMPEDSIFTKINTRIPRDTMPILRRYHLWPDGWNIRGLNGFMLSHKGSEVIDA
                     VIAGQNQAYRELRRIRDNIHSEIYFK"
BASE COUNT         1459 a          784 c          818 g         1097 t
ORIGIN      
        1 gatatcatgc aggaattaaa ttatgagtta ccagagttaa aggcagtaaa atctgaaatg
       61 attatcgcac gtgaaatggg agaaatcttt agctacatgc ctggggaaat agacagttat
      121 atgaaataca taaataataa actttctaaa attgagtaga gaagctcagc cacttgtgta
      181 ggggggctaa aattaattct atttcattat atgagaacac caataagaat aataatattt
      241 gatcacaact gaagatgctt tttacaatga agaattattg ttaaaaaacg ctttgttgaa
      301 tcaaccgaac tgttgctgag ttgcaggatc acatcacgta ttttcctgaa aacgcaggct
      361 gcttagtagc aaagcagaag ttcaaaatca ccaactaaat agctgcgccg aatagtagat
      421 caatgaaagg gaactcagcc cggattgtgc gatctgatca atcgccaaaa atccccccct
      481 aaccggactg agcgatgccg atcatagcac caattccccg cgacgaatga cgcctgatgc
      541 aaaaagcaat ctataaaact cacgataaaa attatgcccg caggctgacc gccatgctga
      601 tgctgcaccg gagcgctcgc gtcagtgacg ttgccagaac gctctgctgc gcccgttcct
      661 ccgtcaggcg ctggattaac tggttcacgc tgtcgagtgt tgaaggtctg aaatcattac
      721 ccgcaggacg ttcccgccgc tggccgtttg agcatatctg cacattgttt cgtgaactga
      781 taaaacactc tcccggatat tttggctatc agcgttcacg ctggagtaca gaactgctgg
      841 caataaaaat caacgagata accgagtgcc agttacatgc cggaaccgtt cgccgctggt
      901 tgccattagt cgggcttgtg tggcgcaggg ctgcgccaac cctgcgtacc cgtaacccgc
      961 ataaagcagc aattcacaaa gcactggacg aatgtagtgc ggagcatccg gtcttttatg
     1021 aagatgaagt gtatatctat cttaatccca aaaccggtgc ggactggcaa cttcgcagac
     1081 agcaaaaaca tgtggtaaca ccggggcaga atgaaaaata ctatctggct ggtgctttac
     1141 atagtggaac gggcaaagtc agctatgtgg gtggcaacag caaaagctca gccttattta
     1201 tcagtctgtt gaaacatctg aaattgacat accgacggga taaacctatc acgctcatag
     1261 tggacaacta catcatccat aaaagtcggg aaacacagcg ctggctgaaa gaaaatccga
     1321 agttcagagt gatttatcag ccagtttact cgccgtgggt gaatcatgtt gagcggttat
     1381 agcaggcgct tcacgatacg ataacgcgca atcatcagtg ccgttcaatg tggtaactgc
     1441 ttaaaaatgt tcattatttt atggaaaccg tgagcccatt ccccggtggt aaatatgggc
     1501 tgttaaaagt gtagcggtat tcggcgcagc tatttaggat ggtcaggtcg cagaagttct
     1561 gtgccactac agtgcgtgta ctaaacaaaa tgacgagggc acgaatgtca taatacgtgc
     1621 gtattacctg acagtagcta ccaactgagg gggcttattc caaacctgat ttattcaaca
     1681 aagctggact tatccttact ctgaattatt ttccagacat atgcatcatg tactggctcg
     1741 cttggttcag gaaatacagg agcaaatcta cagctattag atttattcct atttgtatgc
     1801 aaagtcgcat tgcataatgc ggagctatgt tacagatacg cctgacgtgc gtagtaaaat
     1861 tataaagcaa catatatata caaggagaaa ccgagaaaaa taaatgagtc gtatgttggg
     1921 tagcatcgag ataaatattt tttttatcac attagcacct tggttaaggg tgttaataat
     1981 aatattctct cacttattaa aacataaata attttttaag cggaaatgta attatgagac
     2041 tgccagagaa agttcttttt cctcctgtca ctagtggcct gtcagggcag gaaaaacaaa
     2101 aaaaaccgaa gagcattacc ggatttcagg aaaattatca acgcaatatc aggccaatca
     2161 aaacagcatc agaagcccga ctacgcttct ttgataaaat ggtttcgaaa gaaaactctc
     2221 tggaagatgt tgtttcttta ggtgaaatga ttcagaagga aatttatggg catgaacaaa
     2281 gaacattttc accagttcat catacaggta actggaaatc atcattgtta cacaacgcgc
     2341 tccttggtct ggcaaatgtt tataatggct tacgggaaac agaataccct aacactttca
     2401 acagagatgg tataaaaagt actaactctt ttagagataa cttattgaca aaaacaagaa
     2461 ctcccagaga taattttgag gaaggaataa aacatcctga acatgcaaca ataccatatg
     2521 acaacgacaa tgaaagtaat aaattgctaa aagcaggaaa gatagctggt aacaataacg
     2581 agctgttgat ggaaataaaa aaggaatccc aaagcgacca tcaaatcccc ctgtcagata
     2641 agttcctgaa aaggaaaaaa cgatctcctg tagctgaaga taaagttcaa aactcgttaa
     2701 caccagaaaa ttttgttcag aaaatttcac ttagtgatga gcttaaaaca aaatatgcaa
     2761 atgaaattct agagataaaa agaataatgg gagaatacaa tcttttaccg gataaaaaca
     2821 gtcgtaatgg tctaaaactt ctacagaagc aagctgattt actaaaaata atcatggagg
     2881 atacctctgt tacagaaaat acctttaaaa acatagagat ggctatagca gatataaaaa
     2941 gggagtacta ttcgcataca gttgatattg agaaaaatat tcatgccata tgggttgcgg
     3001 gttccccacc tgaaagcatc tcggactata ttaagacttt tctcaaaacc tacaaagagt
     3061 ttacttacta tctttgggtt gatgaaaaag catttggagc cgcgaaattt accagtgtct
     3121 taaaacaaat tgcatttgat ttagcatgca gaactataca gcagaatact ccacaaaaaa
     3181 atattgattt tattaatcta tataatgaaa taaggaagaa atacaacaac aacccatcgg
     3241 gacaacaaga gtacttaaac aaactcagag agctttatgc tacttatcaa aaaatatcca
     3301 cccctctgaa acacatgttt aattcatttt ttttagaaaa catgattaaa cttcaggata
     3361 atttcttcaa ctattgcatt gtcaaaggtg ttacagagat taatgatgag ttacgaataa
     3421 actaccttaa aaatgtaata aaactgtcag acgatgacat tggtaattat cagaaaacaa
     3481 ttaacgacaa taaagataga gtaaaaaaac taattcttga tttacagaaa caatttggtg
     3541 aaaaccgcat ttcaattaaa gatgttaact ctttaacctc tctttcaaaa tcagaaaata
     3601 atcacaatta tcaaactgaa atgttgcttc gatggaacta tcctgccgcc tcagacctgc
     3661 tcaggatgta tatccttaaa gagcatggtg gtatttatac agacacagat atgatgcctg
     3721 catactctaa acaagtaatt tttaaaatta tgatgcagac aaacggagat aatcgtttct
     3781 tggaagattt gaaactacgt cgtgcaatat ctgatggtgt attaagatat gttaataacc
     3841 aaaatattga tgaagttaac tataatgaaa tcagtgatgc agataaaaac attatcaaga
     3901 agatattaac agaaatatct aaaatgccag aagatagtat ttttactaag atcaatacaa
     3961 ggattcctcg agacacaatg cccatccttc gtcgttatca cctatggcct gatggatgga
     4021 atattcgtgg gctcaatgga ttcatgctat cacataaagg tagtgaagtg attgatgctg
     4081 ttatcgcagg ccagaatcag gcttacaggg aactaagaag aataagagat aatattcata
     4141 gtgaaatata cttcaaac
//
LOCUS       DAZFAB010000208         4136 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZFAB010000208 DAZFAB010000000
VERSION     DAZFAB010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.209"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..79)
                     /locus_tag="ACHJWA_004732"
     CDS             complement(<1..79)
                     /locus_tag="ACHJWA_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO4311161.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTS"
     gene            complement(91..489)
                     /locus_tag="ACHJWA_004733"
     CDS             complement(91..489)
                     /locus_tag="ACHJWA_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO4311162.1"
                     /translation="MTKDELIARLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DETAGQDTPLSRENVLTGHENEVGSAQPDTVILDTSELVTVVALVKLHTDALHVTRDE
                     PVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            complement(531..1556)
                     /locus_tag="ACHJWA_004734"
     CDS             complement(531..1556)
                     /locus_tag="ACHJWA_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO4311163.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVREKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            complement(1612..1944)
                     /locus_tag="ACHJWA_004735"
     CDS             complement(1612..1944)
                     /locus_tag="ACHJWA_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO4311164.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTSS
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            complement(1954..3273)
                     /locus_tag="ACHJWA_004736"
     CDS             complement(1954..3273)
                     /locus_tag="ACHJWA_004736"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO4311165.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPGDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVPAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            complement(3254..>4136)
                     /locus_tag="ACHJWA_004737"
     CDS             complement(3254..>4136)
                     /locus_tag="ACHJWA_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4311166.1"
                     /translation="EDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKAMYAATIES
                     ELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLMPGDSLNLQ
                     TAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANESWAYFMGRR
                     KFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGSGRMAIDGL
                     KEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPPAWAAAAFE
                     SGLRQSTEEEKSDSRAA"
BASE COUNT          814 a         1294 c         1125 g          903 t
ORIGIN      
        1 ctgacgttcc catgtacccg cgtatcgttt catcggcgcg ggcaatggca gcatcgaaca
       61 ggttatcgaa atcagccaca gcgcctcccg ttattgcatt ctggccaggc cgcgctctgt
      121 catttcggct gccacaccgg cagagacacg aaacgccgtt cccggcagca caaatgccac
      181 aggttcatcc cgcgtgacgt gaagtgcatc agtatgcagc ttcaccagtg ccacgaccgt
      241 gaccagttca gacgtatcca gaatcacggt atccggctgc gctgatccca cctcattttc
      301 atgtccggtc agcacatttt cccggctgag aggggtgtcc tgaccggcag tttcatccgt
      361 gtcatcaagc tcctcttcca gctctgccac acggagcgcc agttcttctt tcgtccccgt
      421 caggctgaca tcacggttca gttgttcacc cagcgagcgg agacgggcaa tcagttcatc
      481 tttcgtcatg gactcctcca cagaaaaaca atggccccga agggccatga ttacgccagt
      541 tgtacggaca cgaactcatc agggtcagcc agcagcatca gcggtgctga ctgaatcatg
      601 gtgaactcac gcgccggatc gccggtggtc acccagtttt tcgggtaacg ggcagaggcg
      661 ttaatgcctt cgcgctgtgc gtccgcatcc tgaatgcagc cataggtgcg cagaccgcgt
      721 gcctgagtgt tccccagcac catcgtgttg tccggcagga agttcttttt gacgccgttt
      781 tccacgtact gtccggaata cacgacgatg gccacatcgc catacattcc cttataagac
      841 accgctttgc ccaggtcttt taccgctgtc tccagttcgg aatgagagcc gcgacgggta
      901 tccagcttct ccctgacggc tttgaaggaa cggaacagcg cccagccttt cgggtcaaac
      961 acgatgatat tcaccacgcc gctggcgttc agcgcgtagg cttcgatatc gtcggtcggg
     1021 tcatacgtgg acttgtcacg cttgctccac tccgtgccgc cggactgcgt gatgttgttc
     1081 gccgcactgc ggcccatatc cacctcaacc ggatcgaagg cttcaccggt catggtgtat
     1141 ttgcccttga gcacggcaga aactgcctgc atctcttcga cctgagcaat ggccagctct
     1201 tcgtcacgca tgttctgcat gatgatgcga cggcggcggt aagccgggtc cgccagattc
     1261 tgcggatctt catccggcag gcgacgcagg gtcatctgcg gattcacttc atgcttcggc
     1321 ttgacatatc ccggcgtaaa ttcagaggtg gagccgccac gggaacggat aacctcacca
     1381 gaaacaatcg gcgaaacgta cagcgccatg tttaccagtc ccggaatttg tgagagatag
     1441 actttctccg tggtgaaggg atagctctca cggaaaaaga gacgcagaaa cagcggatca
     1501 aacttaaatt tctgctcatt tgccgccagc agttgggcgg ttgtgtacat cgacataaaa
     1561 aaatcccgta aaaaaagccg cacaggcggc ctttagtgat gaagggtcag gttaaacgat
     1621 gctgattgcc gttccggcaa acgcggtccg ttttttcgtc tcgtcgctgg cagcctccgg
     1681 ccagagcaca tcctcataac ggaacgtgcc ggacttgtag aacgtcagcg tggtgctggt
     1741 ctggtcagca gcaaccgcaa gaatgccaac ggcagcaccg tcggtggtgc catcccacgc
     1801 aaccagctta cggctggagg tgtccagcat cagcggggtc attgcaggcg ctttcgcact
     1861 caatccgccg ggcgcggttg cggtatgagc cgggtcactg ttgcccagcg gctggtaatg
     1921 ggtaaaggtt tctttgctcg tcataaacat cccttacact ggtgtgttca gcaaatcgtt
     1981 aacggcatca gatgccgggt tacctgcagc cagcggtgcc ggtgccccct gcatcagacg
     2041 atccagcgca gtatcactgc gcgcctgtgc actctgtggt gctgcggcca gaatgcggcg
     2101 ggccgtttcc acggtcatac cgggggtttc tgccagcacg cgggcctgtt cttcgcgtcc
     2161 gtgagcctcc tcacagttga ggatccccat aatgcggctg ttttctgccg caaccgcagc
     2221 ggtgatctgc gcgttcacgt ccggctgcgc cgcgctggcg ttttcgccct ccgtcgctgg
     2281 caccacgcca gtaacgtcag cctgcgaagc agtggctgaa acagttgttg attgagtctc
     2341 tttggtcatt cgccctcctg agagacggga tttacgtgca tccagtgcat cacgcataac
     2401 ggtgatcgca tcggtgctgt tgacaagttc atcagccagt ccggcatcaa tggcctcctg
     2461 accgctgtac actgcagcct cggtatccag cacagcctgc acggacaggc cggtatatgc
     2521 cgacaccttc tgcgcaaaca tccggcgggt tgcatccatc cgggactgca gtgtttcccg
     2581 gacatcaccc ggtagatggc tgtaggggtt gccatccacc ttatggctgc cgctgtaaat
     2641 cagcgtgatt tccacgccct gtttctccag ggcagcaccg taattactgt gagccatcat
     2701 gacgccgatg gagcctgtcc gggcggtctg cgtgaccaga cgccgggagg cggcgctggc
     2761 aagcagctga cctgcactgc agttcatgtc gttggccagc gcccataccg gttttatgtc
     2821 tcgcacacgg gcgatgatgt cagcacagtc aaatgctccc gccaccatcc cgcccggtgt
     2881 gtccatatcg agcagaatgc cgtccaccat cggatcgctg gcagcctgtt gcagacgggc
     2941 gataatgccg ttgtaaccgg tcattcccga atacggctgc agcgcccgcg tccggctgac
     3001 cagcgtaccg gacaccggca gcacggcgat gccgttcatg acctgataac tccgggcctg
     3061 tcgtggtccg tcatcatcac cggataacgc cagcgccgcg ggtgcctctc cggcagtcag
     3121 gctgtcgccg gatactgcat ccgtcaggcg gctgatccca agctggcctg caagcgcaca
     3181 aaagaaaacc cgcgcatagg cgggttcaag catcagcggc tcattaaaag ccatgctggc
     3241 aatatgcggg agattacgca gctctgctgt cactcttctc ctcctctgtt gattgtcgca
     3301 gcccggattc aaatgccgca gccgcccagg cgggcggttt aagaccagct gcgcggcgct
     3361 ccatcgtttc acggacctgc tgggcaaaaa tttcctgata gtcgtcaccg cgtttcgcgc
     3421 actctttctc gtaggtgctc agtccggctt ctatcagcat caccgcttcc tgaacttctt
     3481 tcagaccatc gatggccata cgaccggagc ctatccagtc gcagttcccc caggcactgc
     3541 gggcttcctg aaagctgaag cgcgcttttg aaggtaacgt caccacgcgg cgaacgatgg
     3601 cctcttccag ccagcacaga aacatctggc tcgcctgacg ggatgcgacg aattttcgcc
     3661 gccccataaa gtacgcccac gactcgttcg cactggcccg tgccgtggag tagctcatct
     3721 gggcgtaatt ccgggaaagc tgctcatacg agacacccag cccggcagcg atataccgca
     3781 gcagtgactg ctcaaacacg gagtagccgt tatccgtgtc ctgagccgtc tgcaggttca
     3841 gtgagtcccc cggcatcagg tgcggcactt ttgcgcctcc cagacggacc ggtgctgcgg
     3901 cgtaatacgc ggcaatttca ccaatccagc cggtcagcct ttcccgctgc tcctgactgt
     3961 tcgcgcccag aataaaatcc atcgctgact gcgtatccag ctcactctca atggtggcgg
     4021 catacatcgc cttcacaatg gcgctctgca gctgcgtgtt ctgcagcgtg tcgagcatct
     4081 tcatctgctc catcacgctg taaaacacat ttgcaccgcg ggtctgcccg tcctcc
//
LOCUS       DAZFAB010000209         4132 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZFAB010000209 DAZFAB010000000
VERSION     DAZFAB010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4132
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.210"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            35..337
                     /locus_tag="ACHJWA_004738"
     CDS             35..337
                     /locus_tag="ACHJWA_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO4311167.1"
                     /translation="MTLKEFIKSLRVGDAKKFAARLGVSPSYLSQMASGRTAISPTRA
                     LMIESATEGQVSRAELRPHDWELIWPEYASGIRLGQTHVVHAEGDCSACLSDGVDS"
     gene            334..759
                     /locus_tag="ACHJWA_004739"
     CDS             334..759
                     /locus_tag="ACHJWA_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO4311168.1"
                     /translation="MKIKHEHIRMAMNVWAHPDGEKVPAAKITKAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWLDKDTPDAVEKMQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVASAVVLYDQMNRGGPAGNAVVMH"
     gene            782..1744
                     /locus_tag="ACHJWA_004740"
     CDS             782..1744
                     /locus_tag="ACHJWA_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4311169.1"
                     /translation="MSSKLHGLVWEGCAFTGMILSRVAVMARLADYSNDEGVSWPAIE
                     TIRRQIGARSESTVKSAIAELAKEGWLTKEERKVGGRNVSNIYRLNVEKLEAAAAAAR
                     ESYKPKRKISPAKNDPLTVDPSNIAPSTVDPSNFDGSTVDNKLPIRGAMIDPDPSVLK
                     PDPSDKRSSCPDASQPDPQTAEQNFLTRHPDAVVFSAKKRQWGSQEDLVCAQWIWGRI
                     VSLYEQAASDDGEITRPKEPNWTAWANDVRTMRMLDGRTHRQICEMFGRLQRDSFWVK
                     NIMSPAKLREKWDELVIRLGRSPAQRCVNHISEPDTEIPPGFRG"
     gene            1751..2491
                     /locus_tag="ACHJWA_004741"
     CDS             1751..2491
                     /locus_tag="ACHJWA_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000788752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO4311170.1"
                     /translation="MKNIAAVGVLERIRRLAPQGAVPPYRTVEEWREWQLAEGRKRSE
                     EINRLNHQVRVEKILNRAGIQPLHRKCSFGNYRVQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHDGYDNGQSGEKFLQE
                     LCGVDLLVLDEIGMQRDTRNEQVTLNQIVDRRTASMRSVGMLTNLNHAAMSTLLGDRV
                     MDRMTMNGGRWVNFNWESWRSNVGRQGM"
     gene            2517..3286
                     /locus_tag="ACHJWA_004742"
                     /pseudo
     CDS             2517..3286
                     /locus_tag="ACHJWA_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
     gene            3302..3697
                     /locus_tag="ACHJWA_004743"
     CDS             3302..3697
                     /locus_tag="ACHJWA_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO4311171.1"
                     /translation="MAKPFTHEQREELKARIIGLVRKNERMTISQLERATGAGWHSVR
                     RCLVDVLACGDLYMPGKYGVFTSEQVYRVWRKAAEKATDQTLIRKLPDGEIRRYDRQQ
                     NIICGECRKSEVMLRVLAFYQGNFQEAVL"
     gene            3754..>4132
                     /locus_tag="ACHJWA_004744"
     CDS             3754..>4132
                     /locus_tag="ACHJWA_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311172.1"
                     /translation="MTTFTKEQLISHVSENVKAMKFAVKQTAFKNSLEAIELDLALAL
                     VAQASLEAEPVLYMNRFTGKTFSLEEQPGADKEPEIYVPLYAAPPDSAAMLQAGNFRE
                     KKGSSTNNFREISETSTNYPVTLD"
BASE COUNT         1106 a          907 c         1214 g          905 t
ORIGIN      
        1 taaactcgca aaaaaacact aaaccgagga aggtatgaca ttaaaagagt ttattaaatc
       61 attaagggtt ggtgatgcta agaaattcgc ggccagactt ggtgtatcgc catcttactt
      121 atcgcaaatg gcgtctggac gaacagctat atctccaacc cgcgccctta tgatcgaatc
      181 tgcgacggaa ggccaagtaa gtagggcgga gctacgaccc catgattggg agcttatttg
      241 gcctgagtat gcgagcggca ttcgtttggg gcaaacacat gtagttcatg ctgaaggtga
      301 ttgtagtgca tgcttatctg atggagttga ttcatgaaaa tcaagcatga acacatccgc
      361 atggcgatga atgtctgggc gcatccggac ggcgaaaaag taccggctgc gaaaattacc
      421 aaagcgtatt tcgagctggg aatgacgttc ccggaactgt atgacgacag ccatccggaa
      481 gccctggccc gtaataccca gaaaattttc cgttggctgg ataaagacac ccctgatgct
      541 gttgaaaaaa tgcaggctct gttaccggcg atcgaaaagg cgatgccgcc tttgctggtg
      601 gcccgtatgc gcagccacag ttctgaatat taccgtgaga tcgtcgaacg gagggatcgg
      661 ctggtgaagg atgtcgatga ttttgttgcg tcagcggttg ttttgtatga ccagatgaat
      721 cgcggcggcc cggcagggaa tgctgtggtg atgcactaaa agcacggtgt tcgggggttt
      781 tatgagcagc aagcttcatg gtcttgtctg ggaagggtgc gccttcaccg gcatgatctt
      841 atccagggtg gcggttatgg cccgtcttgc agactacagc aatgacgagg gcgtgtcatg
      901 gcctgccatt gaaactatcc ggcgtcagat cggtgcaaga agtgaatcca cagtgaaatc
      961 ggctattgca gaactggcga aagagggctg gctgacgaag gaagagcgta aggtcggtgg
     1021 gcgtaatgta agcaatatct atcggcttaa tgtggaaaaa ctcgaagcag ctgctgcggc
     1081 ggcgcgtgag tcatataaac cgaaaagaaa aattagcccg gcaaaaaatg acccgttaac
     1141 agttgacccg tcaaatattg ccccctcaac ggttgacccg tcaaattttg atggatcaac
     1201 tgttgataac aaactgccga ttaggggggc gatgattgac cccgatccgt cagtattaaa
     1261 acctgatccg tcagataaaa gatcttcttg tccggacgct tcgcaaccgg acccgcagac
     1321 ggctgaacag aattttttaa cccgacaccc tgacgcggtt gtgttcagtg cgaaaaaacg
     1381 ccagtgggga agtcaggaag atttggtgtg cgcacagtgg atctggggac gaatcgtgag
     1441 tctttacgag caggcggcca gcgatgatgg cgagatcacg agaccgaaag aacccaactg
     1501 gactgcatgg gccaatgacg tgcggacaat gcggatgctg gatggcagaa ctcacagaca
     1561 aatttgtgaa atgtttgggc gtctccagcg ggattcgttc tgggtaaaaa acatcatgag
     1621 tccggcaaaa ctccgggaaa aatgggatga actggttatc cgcctggggc gttcgcccgc
     1681 gcagcgttgc gtgaatcaca tttctgaacc ggacactgaa ataccgccgg gattcagggg
     1741 gtgacgtgtc atgaaaaaca ttgcggcagt tggggttctt gaacgtattc gcagacttgc
     1801 accacagggg gcggttccac cgtaccggac ggtggaggag tggcgggaat ggcaacttgc
     1861 tgaaggacga aaacgcagcg aggagattaa ccgcctgaat catcaggtgc gggttgaaaa
     1921 aatcctgaac cgtgcgggca tccagccgct tcacaggaag tgctcattcg ggaactaccg
     1981 ggtgcagaac gacggtcagc gccatgctct gagccaggcg aaatccattg ccgatgaatt
     2041 gatgaccgga tgtacaaact tcgtgttcag cggtaaacct ggtaccggta aaaatcacct
     2101 ggcagcagcg attggcaatc ggctgatggc gaaggggaga agcgtgatta tcgtcaccgt
     2161 gtccgatgtc atgagcgtgt tgcatgacgg ctacgacaac ggccagtccg gggaaaaatt
     2221 tttacaggag ctttgtggag ttgaccttct ggtccttgat gaaattggca tgcagcggga
     2281 tacgcgcaac gagcaggtca cgctgaacca gatagtcgac cgcagaacgg cttcgatgcg
     2341 tagtgtcgga atgctgacga acctgaatca cgcagcgatg agcacactcc tcggagatcg
     2401 ggtgatggac cgtatgacca tgaatggtgg tcgttgggtg aattttaact gggagagctg
     2461 gcggtcaaac gttggacgtc agggtatgtg agaatttttg acgaggtaaa ttttcgatgg
     2521 aaactgtatt gcatgcactg aaagcgatgg gaaaagccaa ttctgttgaa ctggcggcgc
     2581 ggcttgatat cagccgtgaa gaagttctta acgaactgtg ggaactcaaa aaaatggcgt
     2641 tgttgataaa acgggtcaca cctggtttct ggctgtcgaa ggtgaagccg gggtaaccga
     2701 agggcaggca ctacaacctg aagcgccgga tgtggtaacc gaagaggtcg ctccaaaagt
     2761 taccgcagac atgatggttg agtttatcgg tcaggatggt gctaaaacgt gtgaggaact
     2821 ggcgggtaag ttcggcgtca gtactcgcaa ggttgcttcc acgctggcgg tggtaaccgc
     2881 aacggggcgg ctggcacgcg ttaatcagaa cggtaaattt cgttactgca tgtcgggggg
     2941 taatttacca gcagatccga aagccgcgcc ggtaacgaaa aatgatggta aggcctttcc
     3001 tcagccagca ggtgctgcgt taccagtccg ggaagccgca acacaggaag aaattaaaac
     3061 agaaactgtg gcggacattg tgcagccgtt gccatcgttt accgaaacgc aagcagatga
     3121 gctgattttt ccgtccctgc gcagggcaaa cctggcgctg cgcagggcga aaagtgatgt
     3181 tcagaagtgg gagcgagtct gcgccgcgct gcgggagctg aacaagcacc gggatattgt
     3241 tcgacagatt actgattctt cccgccgtgt tgtatcggaa aagtgatagc cggaggcgct
     3301 tatggcgaaa ccttttacac acgaacagcg tgaagaactg aaggcccgaa ttatcgggct
     3361 ggtacgcaaa aatgaacgca tgacgatatc acaactggag agagcgacgg gagcaggctg
     3421 gcattcagtc agacgttgcc ttgtggatgt actggcttgt ggcgatttat acatgcccgg
     3481 taaatacggt gtttttacat cagaacaggt gtatcgcgta tggcgtaagg cagcggagaa
     3541 agcaaccgac cagacattga ttcgaaagtt accagacgga gaaatacgcc gctacgacag
     3601 acaacagaac ataatctgtg gcgagtgccg gaagagtgaa gttatgctgc gtgtactggc
     3661 gttctatcag ggcaattttc aggaggcggt actgtgagtg aattagctat caggcttcaa
     3721 ttgtcgctgg cattcgcatc aaaggagaat gagatgacca cttttacaaa agagcagtta
     3781 atcagtcatg ttagtgaaaa tgtaaaggcg atgaaatttg cagtaaaaca gacagcattc
     3841 aaaaattctc tcgaggcaat tgagttggat ttagcactgg cccttgttgc tcaggcttcg
     3901 ctggaagcag agcccgtgct ttatatgaat cgatttaccg gaaagacatt ctcactggaa
     3961 gagcaacccg gtgctgataa ggaaccggaa atatacgtgc cgctatatgc tgccccgcca
     4021 gacagcgccg ccatgcttca ggctggaaac tttcgggaaa aaaagggttc gtcaaccaat
     4081 aattttcggg aaatctcgga aacgtcaacc aactatccgg taactctgga tg
//
LOCUS       DAZFAB010000210         4011 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZFAB010000210 DAZFAB010000000
VERSION     DAZFAB010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.211"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..281)
                     /gene="escO"
                     /locus_tag="ACHJWA_004745"
     CDS             complement(<1..281)
                     /gene="escO"
                     /locus_tag="ACHJWA_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ATPase activity
                     positive regulator EscO"
                     /protein_id="HFO4311173.1"
                     /translation="MAKINSQIKEVDGKLDDCEQSIKESIASKQAYCASLVNLDKVSL
                     YKYQIKNNAFDEQKQRLYEKKSSLSKEKRSLLDSQKRTKENLQHVNKSV"
     gene            complement(341..1681)
                     /gene="escN"
                     /locus_tag="ACHJWA_004746"
     CDS             complement(341..1681)
                     /gene="escN"
                     /locus_tag="ACHJWA_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ATPase EscN"
                     /protein_id="HFO4311174.1"
                     /translation="MISEHDSVLEKYPRIQKVLNSTVPALSLNSSTRYEGKIINIGGT
                     IIKARLPKARIGAFYKIEPSQRLAEVIAIDEDEVFLLPFEHVSGMYCGQWLSYQGDEF
                     KIRVGDALLGRLVDGIGRPMESNIAAPYLPFERSLYAEPPDPLLRQVIDQPFILGVRA
                     IDGLLTCGIGQRIGIFAGSGVGKSTLLGMICNGASADIIVLALIGERGREVNEFLALL
                     PQSTLSKCVLVVTTSDRPALERMKAAFTATTIAEYFRDQGKNVLLMMDSVTRYARAAR
                     DVGLASGEPDVRGGFPPSVFSSLPKLLERAGPAPKGSITAIYTVLLESDNVNDPIGDE
                     VRSILDGHIVLTRELAEENHFPAIDIGLSASRVMHNVVTSEHLRAAAECKKLIATYKN
                     VELLIRIGEYTMGQDPEADKAIKNRKLIQNFIQQSTKDISSYEKTIESLFKVVA"
     gene            complement(1665..3692)
                     /gene="escV"
                     /locus_tag="ACHJWA_004747"
     CDS             complement(1665..3692)
                     /gene="escV"
                     /locus_tag="ACHJWA_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscV"
                     /protein_id="HFO4311175.1"
                     /translation="MNKLLNIFKKAESYHDLILALFFFMAVMMMIIPLPTVVVDIIIA
                     INISTALLLLMLSIYIKNPLELTSFPTILLITTLMRLSLSVSTTRLILLHHDAGDIIY
                     SFGNFVVGGNIVVGLVIFTIITIVQFMVITKGAERVAEVSARFSLDGMPGKQMSIDGD
                     MRAGVIDPLEAKVLRSRVQKESQFYGSMDGAMKFVKGDAIAGIIIVLVNLFGGVLIGM
                     WQFDMPFSAALSLFSVLSVGDALVAQIPALIISVTAGVVVTRVPGESEKEENLAGDIV
                     QQVSVNSRPFLISAALMLVMAIIPGFPALVFLFLAVCLLGIAWKLQKKRTFGTGNNKD
                     AMGADLSNSQNISPGAEPLILNLSSNIYSSDITQQIEVMRWNFFEESGIPLPKIIVNP
                     VKNNDSAIEFLLYQESIYKDTLIDDTVYFEAGHAEISFEFVQEKLSTNSIVYKTNKTN
                     QQLAHLTGMDVYATTNDKITFLLKKLVLSNAKEFIGVQETRYLMDIMERKYNELVKEL
                     QRQLGLSKIVDILQRLVEENVSIRDLRTIFETLIFWSTKEKDVVILCEYVRIALRRHI
                     LGRYSVSGTLLNVWLIGSDIENELRESIRQTSSGSYLNISPERTEQIIGFLKNIMNPT
                     GNGVILTALDIRRYVKKMIEGSFPSVPVLSFQEVGNNIELKVLGTVNDFRA"
     gene            complement(3689..>4011)
                     /gene="cesL"
                     /locus_tag="ACHJWA_004748"
     CDS             complement(3689..>4011)
                     /gene="cesL"
                     /locus_tag="ACHJWA_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesL"
                     /protein_id="HFO4311176.1"
                     /translation="FLRLLGNDFYLFDNRVEIDFNGFSFFIEIIDNNVFVTFALEYNE
                     NAFFSFFSALAPERTQGVIEHIFVYDNKLCLSCLLTNIDVFFLMNTFQQHVQIIERVR
                     RMTS"
BASE COUNT         1253 a          918 c          715 g         1125 t
ORIGIN      
        1 tctactgatt tattaacatg ttgcagattc tcttttgttc gtttttgtga atctagcaat
       61 gaacgctttt ccttcgaaag tgaagatttt ttctcataca atcgctgctt ttgctcatca
      121 aatgcattgt ttttaatctg atatttatac aacgacactt tatccaaatt caccagcgat
      181 gcacaatatg cctgcttaga ggcaatcgac tctttaatgg attgctcgca gtcatcgagt
      241 ttaccatcca cctcttttat ctgagagtta atcttagcca ttgattctct caatcggttc
      301 gctctgcttt tacgaataga taaaattctg tccaacatac tcaggcaacc actttgaata
      361 ggctttcaat cgttttttcg taactactga tatctttggt gctttgttgt ataaagttct
      421 gaattaattt cctatttttt attgccttat ctgcttcagg atcttgcccc atcgtgtact
      481 caccaatacg aataagcagc tcaacatttt tataagttgc aataagcttt ttgcattctg
      541 ccgccgcacg caaatgctcc gatgtaacaa cgttatgcat aacacgactg gcacttaaac
      601 caatatcaat tgcagggaaa tggttttcct ctgcaagttc tcgggtaagt acgatgtgcc
      661 catcaagaat agagcggact tcatcgccaa taggatcatt aacattatcg ctttctaaca
      721 acaccgtata aatcgcggtt attgaacctt ttggcgcagg ccccgctcgc tctaataatt
      781 tgggtaacga agaaaaaaca ctcggaggga atccccccct tacatcaggt tctcccgatg
      841 caagcccaac atcacgtgcg gctcgggcat aacgagttac agaatccatc attaataata
      901 catttttacc ttgatcgcgg aagtactctg caatagtcgt cgccgtaaat gcggctttca
      961 tcctttccag cgcggggcgg tctgacgttg tgacgaccag tacacattta gaaagcgtgg
     1021 attgaggtaa gagcgcgagg aattcattta cttcgcgacc acgttcgccg ataagagcaa
     1081 ggacaataat atctgcggat gcgccattac aaatcatccc caaaagcgta cttttaccaa
     1141 cgcctgaacc agcaaaaata ccgatacgct gcccaatacc gcaagttagt agcccatcaa
     1201 ttgccctgac gccaagtata aatggctgat caataacctg ccttaataaa ggatctggtg
     1261 gttcagcata caagctgcgt tcaaacggaa gatagggggc agcaatatta ctctccattg
     1321 gtctgcctat gccatcaaca agccgcccaa gtagtgcgtc tccaacacga atcttgaact
     1381 catctccctg ataacttaac cactgaccac agtacatgcc ggaaacatgc tcaaaaggca
     1441 acaagaaaac ctcatcttca tcaatagcga tcacctcggc taaacgttga ctgggttcta
     1501 tcttataaaa ggcgccaata cgcgcttttg gtaaacgcgc cttaataatc gtcccgccga
     1561 tattgataat cttgccttca tatctggtag acgaatttaa tgataatgcc ggcacagtgc
     1621 tattgagcac tttctgaata cgtgggtatt tttccaatac agaatcatgc tctgaaatca
     1681 tttaccgttc ctaatacttt aagttcgata ttattcccaa cctcctgaaa agagagcacg
     1741 gggactgacg ggaacgaacc ttcaatcatt ttcttcacat agcgcctgat atctaaagcg
     1801 gtcagaatga cgccgttccc cgttggattc atgatatttt ttaagaagcc aattatctgc
     1861 tcagttcgct ccggtgagat attcagatac gaacctgatg acgtttgtct gatcgactct
     1921 cgtagctcat tttcaatatc agagccaata agccaaacgt tcagaagtgt accgctaacg
     1981 ctatagcgac ctaaaatatg ccgacgcagg gcaatacgaa cgtattcgca caagataacc
     2041 acatcctttt cttttgttga ccaaaaaata agcgtctcaa agatagttct caggtctcta
     2101 attgagacat tttcctctac gagacgttgt aggatgtcaa caattttgct caaaccaagc
     2161 tggcgctgca gctctttcac aagctcgtta tattttctct ccatgatgtc catcaaataa
     2221 cgcgtttctt gtacgccgat gaactctttg gcattagaca atacaagttt ctttagcaaa
     2281 aacgttatct tatcatttgt tgttgcataa acatccatac ctgtaaggtg agcgagctgt
     2341 tgattagtct tattcgtctt atatacgata gagttagtcg aaagcttttc ctggacaaat
     2401 tcgaatgata tctctgcatg cccagcctca aaatagacag tatcatctat aagagtatct
     2461 ttgtatattg actcttgata gagcaaaaat tctattgcgc tatcattatt tttaaccgga
     2521 ttaacaataa tcttaggcaa tggaattcca ctttcctcaa agaaattcca acgcatgacc
     2581 tcaatttgct gtgtaatgtc tgaactataa atattactac ttaagtttaa aatcaatggc
     2641 tcagcgcccg gtgagatgtt ttggctatta gacaaatcag ctcccatagc atccttatta
     2701 ttgccagttc caaatgttct tttcttctgt aatttccagg ctatccccaa caggcaaacc
     2761 gccagaaata agaagaccaa tgcaggaaag cccggaataa tcgccattac gagcattagt
     2821 gcggcactga ttaaaaaagg gcgactatta acagaaacct gttgaacaat atcacctgca
     2881 aggttttctt ctttttcgct ttcaccgggc acacgagtaa caaccacgcc tgcggtgaca
     2941 gaaataataa gcgcagggat ctgggcaacc aaagcatcac cgacagacaa tacagaaaac
     3001 aggctaagtg ccgcactaaa tggcatgtca aattgccaca taccaatgag cacgccacca
     3061 aaaaggttaa ctaaaacaat aatgatacca gcaatagcgt ccccttttac aaacttcatc
     3121 gccccgtcca ttgaaccata aaactggctt tccttttgca ctcgggagcg aagaactttt
     3181 gcctcaagcg ggtcaataac gcctgcgcgc atatcaccat caatactcat ttgctttcct
     3241 ggcataccat caagggaaaa acgagcactc acttcagcaa cacgctcggc cccttttgta
     3301 ataaccataa attgaacaat ggtgataata gtaaaaataa ccaggccaac aacaatatta
     3361 ccgccaacaa caaagttacc gaaggagtaa ataatgtcac ccgcatcgtg atgcagcaga
     3421 ataagtctgg ttgtactaac actgagcgac aggcgcatca acgtcgtaat caacaggatt
     3481 gtggggaaag aagttagttc aagaggattt tttatataga ttgagagcat taataaaagt
     3541 aaagctgtcg aaatattaat cgcgataatg atatccacca caactgttgg tagtggaata
     3601 atcatcatca ttacagccat aaagaagaag agagccagaa taagatcgtg atatgactct
     3661 gcttttttaa atatatttaa gagtttattc atgatgtcat cctgcgaacg cgctcaataa
     3721 tctgcacatg ctgttgaaaa gtattcatta gaaaaaaaac atcgatattg gttaacaaac
     3781 aacttaagca caatttatta tcgtagacaa aaatatgttc aatcactccc tgcgtacgct
     3841 caggagcaag ggcactaaag aaagagaaaa atgcgttttc gttatactct aaagcaaacg
     3901 taacaaaaac attattatca attatttcaa taaaaaaaga aaacccatta aaatcaattt
     3961 caacacggtt atcaaataaa taaaaatcat ttcccaataa tcttaaaaac t
//
LOCUS       DAZFAB010000211         3993 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZFAB010000211 DAZFAB010000000
VERSION     DAZFAB010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.212"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..572
                     /locus_tag="ACHJWA_004749"
     CDS             <1..572
                     /locus_tag="ACHJWA_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO4311177.1"
                     /translation="KLVIISVMILYVMSGSRLADNHTLSAGYAQSKVQDFKNIKGVNL
                     QYRYEWDSPVSVVGSFSYMKGDWADSHRDEADDFYRHQADIKYYSFLAGPAYRLNDYI
                     SFYGLVGISHTKAKGDYEWRNSVGADESDGYLSESVSKKSTDFAYAAGVIINPWGNMS
                     VNVGYEGTKADIYGKHSVNGFTVGVGYRF"
     gene            708..1499
                     /locus_tag="ACHJWA_004750"
                     /pseudo
     CDS             708..1499
                     /locus_tag="ACHJWA_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308008.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
     gene            complement(1504..2874)
                     /locus_tag="ACHJWA_004751"
                     /pseudo
     CDS             complement(1504..2874)
                     /locus_tag="ACHJWA_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(2894..3241)
                     /gene="tnpB"
                     /locus_tag="ACHJWA_004752"
     CDS             complement(2894..3241)
                     /gene="tnpB"
                     /locus_tag="ACHJWA_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO4311178.1"
                     /translation="MITLPTGTRIWIIAGITDMRCGFNGLASKVQNTLKDAPFSGHIF
                     VFRGRSGKMVKILWADRDGLCLFAKRLERGRFVWPVTREGKVHLTPAQLSMLLEGIAW
                     QHPKRTERPGIRI"
     gene            complement(3238..3912)
                     /gene="tnpA"
                     /locus_tag="ACHJWA_004753"
     CDS             complement(3238..3912)
                     /gene="tnpA"
                     /locus_tag="ACHJWA_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFO4311179.1"
                     /translation="MNSQTKKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFIASGIAWPLPDSVSLAQLDAILYANRKKELTEPQISEGTWRKERRTSYSRE
                     FKIRLVKQALQPGAVVARIAREHGINDNLLLKWKSQYEDGLLSDDDIQECMPVPVALT
                     DTPEPTRPVTNPFWRNKPDECPESDPGNVPRCELHLKSGVVKLFDPLTPEMLRALIRE
                     MKGGTR"
BASE COUNT          900 a          940 c         1080 g         1073 t
ORIGIN      
        1 aaaaactcgt tataatttca gtaatgatat tatatgttat gagtggttca agactggctg
       61 ataatcatac gctatcggct ggttatgctc agagtaaggt acaggacttt aagaatatta
      121 aaggagtgaa tctgcaatat cgatatgagt gggattctcc ggtcagtgtc gtcgggagtt
      181 tttcgtatat gaaaggtgat tgggcggaca gtcatcgcga tgaagctgac gatttttaca
      241 gacatcaggc tgatattaaa tattattcat ttctcgcagg cccagcttat cgtctgaatg
      301 actatatcag tttttatggt ttggtgggga tatctcatac taaagcgaag ggagattatg
      361 agtggagaaa cagtgtgggt gctgatgagt ctgatggata tttaagtgag tctgtatcaa
      421 aaaaatccac tgattttgct tatgcagctg gtgttattat taatccgtgg ggcaatatgt
      481 ctgtaaatgt tgggtatgag ggaactaaag cagatatata tggaaaacat tcggtcaatg
      541 gatttactgt tggagttggt tatcgttttt aatcattaga cgggactgga taattgcact
      601 tgcgtagtga agtttactaa atgtttgtat tttaatgaag tattcgacat attcccttca
      661 gacggaatat gtcgacggtt atggttttac tggagcatga gtatgttatg gcgaaactga
      721 taaatatatc agcgtccact gaggagagaa tgacgccagg ggagaggagg gtggcttcac
      781 gactggagag ctttctgaat gatgattgct ttgtctggta cgatatcccg gtgggacgga
      841 aaaaccgaca tccggatttt gtcattattg acccggataa cgggctggta tttctggagg
      901 tcaaggactg gactgtatcc acattacgta aagcaaatca ggagcaggtt acactggaaa
      961 ccgacggtct tctgaaatca gaaataaatc cgctggtaca ggttcgtcgt tatgcctgtg
     1021 atacggttaa tgccttacct gctgacccgt gcttacggca gaatgacggt cagtataagg
     1081 ggcggctgaa cctggcctgg gcgtatggtg tggtgtttac ccgcattacc cgtcagcaac
     1141 tgaaggcact gacagggaat aatgagaatg cggtggaaaa gatttttcct tcagcacaga
     1201 caatctgtca ggatgaaatg acgcagtctg ttttacctga ggtattccgg caaaaaattg
     1261 ccgggatgtt taccacgggg ttcagaaccc gggtaacacc ccggatgagg gatattctgc
     1321 gcgcccatct gtttcctgaa gtgaccgtga aacagaactc acaaatcaaa attatggata
     1381 ttcagcagga gattctggcc agaaatattg gtgacgggca tcgtgtcatt catggtgtgg
     1441 ccggttccgg aaaaaccatg attcttttgt tccgttgcct ttacctggca gaaaccacgt
     1501 ccggtagtta cgccgcccca gtgcaactac ccggagggcg ttttcacaca ggttattgtc
     1561 gatctccacc cagccattgc ggcagtattc gttcagagcg tcccattgct tcagcagata
     1621 ggtgaacgct ttcgctgtat ccgagtggcg cgacagcgtg ttcatctgcc cctggagcca
     1681 ctcatacaac gactgcatta gcggtaccgt tctggctttt ctgaccgcca gtcgctcttc
     1741 tgccggactg ccgcggatct cagcctcgat agcgtacagt tccccgatac gacggagcgc
     1801 ttctcccgtt actgtcgttg gatggcggac atgtacatcg tggatcttgc gtcgggcatg
     1861 tgccatacaa gccgcttcgg ttgcctgacc gctttcgtaa agagcattgt aacccgcata
     1921 tgcatcggcc tgcaggatac ctctgtagtc cgccagatgt tgctgtgggt ggatgccttt
     1981 gcggtcggga gagtatgaga accacaccgc tgccggcatg gtcgaaccgg cgttgcgatc
     2041 gtcacgaaca tagacccaca gccgtccggt acgggtttta ccctgacccg gctccagtac
     2101 atttaccggc gtgtcatcgg catgcacttt acccggcatc agcacatagt gcttcagttc
     2161 atcatacagc gggcgaagct gctctcccat gatgtcaacc cagcgcccca tcgtattgcg
     2221 gtgcagctcc acgccctggc gggcatagat ttccgactga cggtacagcg gcagatgctc
     2281 ggcgaactta gccatgatta tgcgggccag cagagccgga ctggcgtaac tgcgctcgat
     2341 gggttttggt ggctgcggag cctgaactat acagtcgcac cggctgcagg cccgttttgg
     2401 gcgaaccgtt tcgattaccc tgaacgcggt gttgatgata tccagttgtt cagagatgct
     2461 ttctcccagc ggtttcagtt taccgctgca gacggggcat tcggtttctg ccggggagat
     2521 aacctgcctg tcacggggaa gtgttgccgg aagtgctttg cggacgggag agtctgatgt
     2581 tttcggcgct gtctctccgg ccattgaggt gagttgcaac tgcgcctcac caagcctgtt
     2641 ctggagctcg gttatacgcg tttctgcccg tgcgatcttc ttttctatct tctcgcggct
     2701 tttctcgctg ctgcgaccga acaacattct ctgcagttta gcgaccagcg ctctgagtga
     2761 gctgatctcg cgggcatagc cggttatttc accagacaga cggacgataa cagcctgctg
     2821 tgcgatcagc agggccttca gttgctcgat gttgtcgggg agtgtgttgt tcattcccct
     2881 gttttatcac gggttatatc cggatgccag gccgttctgt ccgtttggga tgttgccatg
     2941 cgatcccctc cagtagcatg gataactgag ctggcgtcag gtgcactttc ccttcccggg
     3001 ttaccggcca gacgaagcgg ccccgttcca ggcgtttggc gaacaggcat aacccgtcac
     3061 gatcggccca cagtattttc accattttgc cactgcggcc ccggaagacg aagatatgcc
     3121 cggagaacgg ggcatctttc agcgtgttct gcaccttcga agccaggccg ttgaagccac
     3181 aacgcatatc tgtgatgcca gcgatgatcc agattctggt accggttggc agcgttatca
     3241 tcgggtacct cctttcattt cgcggattag cgcccgtaac atttccggag tgagagggtc
     3301 aaacagtttt accacacctg atttaagatg cagctcgcac cgtgggacgt ttccgggatc
     3361 actctcaggg cactcatcag gcttgttacg ccagaaggga tttgtaactg gtctggtcgg
     3421 ctctggcgta tcagtcagag ccaccgggac aggcatgcat tcctgtatgt catcatcgct
     3481 cagtaagccg tcctcgtact ggcttttcca ttttaacagc aggttatcat tgataccgtg
     3541 ctctctggcg atccgggcaa caacagcacc gggctgtaat gcctgtttta ccagcctgat
     3601 tttaaattca cgactgtagg aggttcgtcg ttcttttcgc catgtgcctt cgctgatttg
     3661 aggctctgtt aattccttct ttctgttggc ataaaggatg gcgtcaagct gagctaatga
     3721 aactgaatcg ggcaatggcc atgcgatacc ggatgcaata aatcgctgaa aaagcgtatg
     3781 tattgtggaa tgactgagac ctagacgctg agcgatggcc cggatggtca gtttatcttc
     3841 aaatcttaaa cgcagagcat caggcaaata agaacggaag cagggaatat ctttttttgt
     3901 ctgggaattc atcgttcgtg tccatctata tagatgggcg cgattgttgc cagacaggac
     3961 aattttcaca agacgtcgca gatggggcgc tta
//
LOCUS       DAZFAB010000212         3952 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZFAB010000212 DAZFAB010000000
VERSION     DAZFAB010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3952
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.213"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            289..390
                     /locus_tag="ACHJWA_004754"
                     /pseudo
     CDS             289..390
                     /locus_tag="ACHJWA_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(800..1035)
                     /locus_tag="ACHJWA_004755"
                     /pseudo
     CDS             complement(800..1035)
                     /locus_tag="ACHJWA_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1194..1844)
                     /locus_tag="ACHJWA_004756"
     CDS             complement(1194..1844)
                     /locus_tag="ACHJWA_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001310425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFO4311180.1"
                     /translation="MPFSFNLSSGNYLSTQDVEVLQRATRDHQMERLTIGERSFSVRY
                     QSAMDAFIVDPVQGELYSGLSHTELADIIRLADSVENQLNGGNSFLDVFSTYMGQVIS
                     EFMHSNDNRIELLQRRLHSCSFLVNIEEMSYIDEALQCTITLAIPQRGVFLRNAEGSR
                     VCSLYDEMALSRIINDGMHHPLSREPITLSMLVAREQCEFDCSIGHFTVRSDCYSV"
     gene            2076..3132
                     /locus_tag="ACHJWA_004757"
                     /pseudo
     CDS             2076..3132
                     /locus_tag="ACHJWA_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(3351..3941)
                     /gene="espM1"
                     /locus_tag="ACHJWA_004758"
     CDS             complement(3351..3941)
                     /gene="espM1"
                     /locus_tag="ACHJWA_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector guanine nucleotide exchange factor
                     EspM1"
                     /protein_id="HFO4311181.1"
                     /translation="MPVNATGVSFSSFGISYHKDNSFRGTIRGKNDEVVKCSMGERSI
                     RFNVNKFSGCILETVSRQSTKDIHGWVSDERTVYPSRVINQEIDNCCLQKNAKISSEE
                     RKMVFSLVSKEFELTLDVKAAQSSINHIIIGNASFGKKMDALCDGMSRAVKNSTTDYI
                     ANVLADKFYQKHIAPGVDIVKLRNEIPGYMSRVIQG"
BASE COUNT         1187 a          917 c          726 g         1122 t
ORIGIN      
        1 acggtagtaa caccagattt atacttagcc gtggtatcga tatccatgta ccacactggt
       61 agattttaca aaactcaata acatattata tttaaaggaa aaatacagaa tttacgatgc
      121 ttttttatac agatctctcg tgtttttaac tgattgattt tgttgaactc tttttttgtt
      181 ttgcaggagt tactcctctt accttattgt ccactcgatg accaacacat tcacaaccca
      241 aaccagaata tcctatctat gaagcatgta tccgcacgca gattaattat ggtttacatt
      301 gttctaccac aatagtcgca tcctaaatgg gtgcggttga ggatcaccat ccttgaagag
      361 gatgcccgcc actttaactg catgacaggg ctatttaaac aatcactaaa aatcgcccaa
      421 gagcattctg tttctataat gtattccaag agattaaaaa ataatatcat acgttattcg
      481 gcagagttcg tatgtactac ggcgtcccaa ccatgtccca aaacaaaaat ttctgcccta
      541 tacacgttcc aaaaaattca acagcaaaga gagtctgctg acgagttgtt gattcactga
      601 ttaatacgta aaattgacaa ctgctggaaa tcatacaata ctcgtacttt cgaaagttcg
      661 ctaaccaggt gctacacgcg aggtgttaca gttttcttta cgatttatca ttcaatccta
      721 atccatgcat gacatgtggt gcctgtgggg tgatcctacc tacgtaatgt ggacacagcc
      781 ctaatcaagg ttctgttttt caaaacagtt ccgtgctgag acagccacaa gtactgtgtc
      841 ggcaccaccg attgtaatca cactagatct aattaaacac agccgtctgc attatttccc
      901 gactcataaa atattagccg taaatgcatt cgacttttag tgagtggaag aaactttccg
      961 ccctagcatt atcgtaacag cgcccctttg cactcatcct gtcatgcagg tatgccgctt
     1021 cagaagcgcc tgataatgcg ttgaaatcag gacggtggct acttgctatt cagttcacga
     1081 tacatccctt aaggaccagc gaatagcaat gagtcacttt attatcaagt ttgatgaccg
     1141 ctttgatgct aacttttaat aagagtctct tcacacaaaa tcgtgtaccg agtctacact
     1201 gaataacaat cactcctcac cgtaaagtga ccgatactgc aatcaaactc acactgctct
     1261 ctggccacaa gcattgataa tgttattggc tctctgctta gtgggtgatg catcccatca
     1321 ttaattatac gagaaagagc catttcatca tataaactac atactctgga accttcagca
     1381 tttcttaaaa aaacacctcg ttgaggaatt gccagcgtaa tcgtgcactg taatgcttca
     1441 tctatgtaag acatttcttc aatattaact aaaaatgaac atgaatgtaa tcgccgctgt
     1501 aacaattcaa ttctgttatc attactatgc ataaattcag aaataacctg ccccatatat
     1561 gtactgaata catcaagaaa tgaattgcct ccattcaatt gattttcaac agaatcagcc
     1621 aatctaatga tatcggctag ttctgtatgg cttaaacccg aatatagttc cccttgaaca
     1681 ggatctacaa taaaagcgtc catagcactc tgatagcgaa cagaaaacga tctttctcct
     1741 attgtgagtc tttccatttg atgatctctg gtcgcacgtt gtaaaacttc aacatcttgt
     1801 gttgacaagt aatttccaga agacaaatta aatgaaaatg gcataatttt atccctaaag
     1861 catatatctt acttttcgat aattaacaaa aagccatcct ccacagcaat actccatcag
     1921 aataattgta ggattatttt aatctaattt ataagcataa aatatatact cactaaatag
     1981 ctgcgcagaa tagtagatca tttagaggga actcaacccg attgtgcgat ctgatcaatc
     2041 gccaaatcaa tacaaatcac caaccggact gagcaatgtc gatcatacca ccaatttccc
     2101 gtgacgaacg acgcctaata cagaaagcca tccataaaag gctgacagcc atgctgatgc
     2161 tgcaccgggg cgaccgtgtc agcgacgttg cccgcacact atgctgcgcc cgttcctctg
     2221 ttgggcgctg gattaactag ttcacacagt cgggtgttga gggactgaaa tcattacctg
     2281 ccgagcgagc ccgccgctgg ccgtttgcgc atatctacac actattacgt gaacaggtaa
     2341 aatattcact cgcgactttg gctacaatta ttcacgctgg agtacagaac tgctgacaat
     2401 aaaaatcaat gagataaccg gctgcctgtt acatgctgga accattcgcc gctggttgtc
     2461 gtctgtgggg cttgtgtggc gaagggctgc gccaactcta cgtatccgtg ccccgcctaa
     2521 agataaaaag atggcaacaa tccataacgc actggacgaa tgcagcacag agcatccggt
     2581 cttttatgaa gatgaagtat ttatccatct taatcccaaa atcggtgcgg actggaaact
     2641 gctcgggaag caaaaacgag gggtcacgcc ggaacaaaat gaaaaatatt ctctggacgt
     2701 agcgctgcac agcgggacag gttaagtcag ctgtgtgggc gccaacagca aaagttcggc
     2761 gctgttcatc agactgctga agcggcttaa agctacatac tgtcggacga aaaccatcac
     2821 gctgctcgag gacaactaca ttatccacaa aggccgggaa acccagcgct ggctgaagga
     2881 taaaccgaag tgcagagtca tttatcagcc ggtttaatct ccatgggtga atcattttga
     2941 atagctatgg cagatacttc acgccacaat aacgcgtaat catcagtgcc gctcaatgtg
     3001 gcaactattg aaaaagttcg ccattttatg gaatccgtca gtccattccc cgaaggcaaa
     3061 catgggctgg caaaaatgtg cgatattagg cgcagctatt tagttattcc gtcatttctc
     3121 ccgcccgtgt aaacactact gaatcttgtc gttgtgacaa agctataaat agtcaatcac
     3181 agcacgtttc tgcgtacgac gcgctgcaat tttctttacg atttataact gtacaggtga
     3241 actatgagtt atttggatac atacatatag tgtaacgcct gttgtatatt ttactcatat
     3301 ctttttacag gatgaaaacc aatgatactg gcgcatagcc tcctccaacc ttacccctgt
     3361 ataacacgac tcatataacc tgggatttca tttcgtagtt ttacaatatc aacacccggg
     3421 gcgatatgtt tctgataaaa cttgtctgca agcacgtttg ctatgtaatc tgttgtactg
     3481 ttttttacag ctcggctcat accatcgcaa agagcatcca tttttttgcc aaaagaagca
     3541 tttcctatta ttatatgatt aatagaactt tgtgccgctt taacatcaag agttagttca
     3601 aactccttac ttacaagaga aaagaccatt ttcctctctt cagaagaaat ttttgcattt
     3661 ttttgaaggc agcaattatc aatctcttgg ttgataaccc ttgacggata tactgtccgt
     3721 tcatcactta cccatccatg aatatcttta gtactctgcc tgcttaccgt ctccagtata
     3781 cagccgctaa atttgttaac attaaagcgg atagagcgct ctcccattga acacttcacg
     3841 acctcatcat tcttcccacg gatggtgccc cggaaagaat tatctttatg ataactgata
     3901 ccaaaagagc tgaaggacac acctgtcgca tttactggca taacaaatcc tt
//
LOCUS       DAZFAB010000213         3886 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZFAB010000213 DAZFAB010000000
VERSION     DAZFAB010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.214"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..925)
                     /locus_tag="ACHJWA_004759"
     CDS             complement(<1..925)
                     /locus_tag="ACHJWA_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HFO4311182.1"
                     /translation="MATWQQGINSGGFLAGIGGQNSNAPKASDVSEALAYIRQNNEME
                     RSGRNNIGLQALQGLGSVAQTSQAAKQQEADAAFQKEYAAAIQSGDRQQVRDLMTKYP
                     GQLEKIQSGMKWADEDQRNSIGTLAAGARLAASSPEAMQSWLQNNAKELARVGVDPNN
                     VAQMYQQNPSGFGEFVYHLGMAALGPIDYFNVQDKMAGREIDRGRLAETIRSNQAGDF
                     EFKSKPSYLVLSFAEISSVVFILMSVYMVAKSQNSNGLQYDALFIPSMAFSILVFSFN
                     GGIISKIISNKVMILLGDASFSFYLVHTIVIS"
     gene            complement(935..1615)
                     /locus_tag="ACHJWA_004760"
     CDS             complement(935..1615)
                     /locus_tag="ACHJWA_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFO4311183.1"
                     /translation="MLVLSESFKNKLLPMNGYMKGGSDSGSKAQARATEKGIELQREM
                     WQTNMQNLAPFTPLAQQYVSQLQNLSSLQGQGQALNQYYNSQQYKDLAGQARYQSLAA
                     AEATGGLGSTATGNQLAAIAPTLGQNWLSGQMNNYNNLANIGLGALTGQANAGQNYAN
                     NVSQLYQQQAAASAANANKPSGLQSFATGAIGGAASGAMIGSAVPVIGTGIGALAGGV
                     IGGLGSLF"
     gene            complement(1602..2069)
                     /locus_tag="ACHJWA_004761"
     CDS             complement(1602..2069)
                     /locus_tag="ACHJWA_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HFO4311184.1"
                     /translation="MEIKLIDNPVKLAEFLNNPVNTGNIVDSGDKYYIKPDAVYLGIY
                     EGLVLAGVHEVRNFWHSVVECHAVYDPGFRGEYALQGHRLFCKWLLENSPFLNSITMV
                     PDTTKYGRAIIRLLGATRVGHLDDAYMSNGKPVGITLYQLPRSKYEELLNVST"
     gene            complement(2069..2605)
                     /locus_tag="ACHJWA_004762"
                     /pseudo
     CDS             complement(2069..2605)
                     /locus_tag="ACHJWA_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail needle knob protein"
     gene            2669..3316
                     /locus_tag="ACHJWA_004763"
     CDS             2669..3316
                     /locus_tag="ACHJWA_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO4311185.1"
                     /translation="MVNERSINGQAGKIVSKWVTLANNAIPSNVPPQWDLDLKGQQYS
                     EPTKCIGKQIDYMAKPETKLNDFNREASKSLYDWSDANVWGYSNGWARHEIKKETLSI
                     TGFHAVRRILKAVQASRKDNYKAKRQIQRTLKRQDINRSPITPFQAPLISKHDWDLSV
                     IAVTKEAETERRELLSSQIINSNKVQTHPLLRMLRRARVRASRAKRESGGYDHYY"
BASE COUNT         1110 a          899 c          887 g          990 t
ORIGIN      
        1 tactgattac tatagtatgg accaagtaaa atgaaaaaga agcatcacct aaaagtatca
       61 taactttatt tgatattatt tttgaaataa taccaccatt aaacgaaaac actaatattg
      121 aaaaggccat tgatggtata aaaagcgcat catactgtaa tccattgcta ttttgacttt
      181 ttgccaccat atagacagac attaggataa agactacaga tgatatttca gcaaatgata
      241 gaactaaata tgacggcttt gacttaaact caaaatctcc ggcctgattg ctgcggattg
      301 tctctgccag cctgcctcgg tcaatctcac gaccagccat cttgtcctga acattgaagt
      361 agtcaatcgg accgagagca gccatcccaa ggtgataaac aaactcacca aaccctgaag
      421 gattctgctg atacatctga gcaacgttgt tagggtcaac accgacgcgc gccagttcct
      481 tggcgttgtt ttgcagccat gattgcattg cttctggaga cgaggccgca aggcgtgcgc
      541 cagccgctaa ggtgccgata gaattgcgct ggtcttcgtc tgcccacttc ataccagact
      601 gaatcttctc taattgacca ggatatttgg tcatcagatc tcgaacctgc tgtcgatcgc
      661 cggactggat ggctgccgca tattcttttt ggaatgcagc atccgcttcc tgttgctttg
      721 cggcttgaga tgtttgagcg acactaccaa gtccctgcaa cgcctgaagg ccgatgttat
      781 tgcgacctga acgctccatt tcgttgttct ggcgaatata ggccaacgcc tcacttacat
      841 cacttgcctt tggcgcattt gagttttgcc caccgatacc agcaagaaaa ccgcctgagt
      901 tgattccttg ttgccaagta gccatattcc caccttaaaa caatgatcca agaccaccga
      961 taacaccgcc agcaagagca ccaataccag tcccaataac aggaactgca ctaccaatca
     1021 ttgcacctga tgcggcccca ccaatggcac ctgtagcaaa actctgtagg cctgaaggct
     1081 tattcgcatt tgctgccgat gctgccgcct gctgttgata caattggctg acattgttag
     1141 cgtagttctg tccggcgttt gcctgacctg taagagcacc aaggccgata tttgccagat
     1201 tgttgtagtt gttcatctga cctgacagcc agttttgacc gagtgtaggt gcgattgctg
     1261 ctaactggtt tcctgttgct gtagagccta atccacccgt tgcctctgct gctgccagac
     1321 tctgatagcg cgcctgccct gcaaggtctt tatactgctg agagttgtaa tactggttaa
     1381 gcgcctgacc ttgcccctga agagaggaaa gattctgcaa ctgtgatacg tactgctgag
     1441 cgagtggcgt gaacggtgca aggttttgca tgttcgtctg ccacatttca cgctgcagtt
     1501 cgatgccctt ttcagttgcg cgtgcctggg ctttagatcc ggagtcgctg ccgcctttca
     1561 tatacccatt catgggaagc aatttattct tgaagctttc gctaagtact aacatttaat
     1621 agctcctcat atttcgaacg aggtaattga tagagggtga ttcctaccgg ttttccgtta
     1681 ctcatgtacg catcatcaag gtgaccaaca cgggtagcgc caagcaaacg gataattgcc
     1741 cgtccgtatt tggtggtgtc aggaaccatg gtgatgctgt taaggaatgg tgagttttcg
     1801 agaagccatt tgcagaataa tcgatgccct tgtagtgcat attcgccacg gaatccgggg
     1861 tcatacaccg catggcattc aacaacgcta tgccagaagt tacgcacttc atgaactcca
     1921 gccagcacta atccttcgta gatgccgaga tataccgcat caggcttgat gtagtattta
     1981 tctccactgt ctacgatatt ccccgtgttt accgggttgt taaggaattc tgcaagtttc
     2041 accgggttat cgatgagctt tatttccatc actgctccgc aatgattttg atggttgtgg
     2101 cagtaaacgc cgcgccattc gactgaatgg ttaacgtgct gccatttgtg gcaagaaagc
     2161 cgtctttatc cacgctgaag aacgtagcta acaggatgtt gtcggttgtt gttgccgagt
     2221 tgcgactgct tactagcgtg tcaggaacag aaccggagaa tgttagctgc attgacctgt
     2281 tggcggttcc gctgggccac gtcccgacaa tcgacagctt gaagaacaag gttttgttct
     2341 cgttgaacac aaccatcttg ttgttaacgg tgtcgaagaa tggtgccaac gtcccggatg
     2401 acggcgtaag cgttttcagc aggctaacaa ggttggtcgg cgctgtcggg atggttactg
     2461 atacgccaga gtaaacaacc tctgacttct tgcgcgtagt ggcatactcc agagcattaa
     2521 tgcgcgtttc atggtctgaa acctgcgatt ccagcgactg aactctggta tcaagcgatg
     2581 caatatcgct ttcattcaag gcaatcagtg ggtttacgtc atggagtggg ctcaacacca
     2641 gtcagagccg catgtacacg caataatcat ggtgaacgaa agaagcatta atgggcaagc
     2701 tggcaaaatt gtcagcaaat gggtaacact ggcaaacaat gcaatcccat caaatgttcc
     2761 tccccagtgg gatctagatt tgaaaggtca gcaatattca gagcctacaa agtgtatagg
     2821 taaacagatt gattacatgg caaagcccga aactaagtta aatgacttta atagagaggc
     2881 gagcaagagc ctatatgatt ggtcagatgc caacgtttgg ggttattcga atggctgggc
     2941 gcggcatgag ataaaaaaag aaacactttc tatcactggc tttcatgctg taagacgcat
     3001 actaaaagcc gttcaggcat caaggaaaga caactacaaa gctaagcgac agatacagcg
     3061 tacattgaaa cgacaagaca taaatcgatc accaatcacc ccgttccaag caccgttaat
     3121 tagcaaacac gattgggatt tatcagtgat agcggtcacc aaagaggcag agacagagcg
     3181 cagggaatta ctatcctcac agataataaa ctcgaacaaa gtacaaaccc acccactgtt
     3241 aaggatgtta agaagagcac gagtgagagc atccagagcc aaaagagaaa gcggaggtta
     3301 tgaccattac tactagtagt gacagatcgt gacaaggcgg tgcaaaaaac gaccaataat
     3361 aagagagata tgcctgccaa accgccaaaa gcgcggagcg cggcaaacgc cgcgagaggc
     3421 gcaagaaggc gaacctatcc attgaaacgg tcagaagcgc atcgcgctcg acgaccgaag
     3481 tccccactaa caccgttaac gaaagatcaa agtcttagaa aaaaatgacg tttctttgat
     3541 ggacttccat ccgtaaaaat caaattttga gaaaaagtac ttatagaaat taatcgctct
     3601 ataaggataa gttgacgtta gtttaaagtg ataaaacatt aaagaaaaaa cgtaaaatca
     3661 aatacataaa ataagtcacc aagggatcat tttcagaaaa caacgtcaac gtaacgtcaa
     3721 ctctgtaaga acttttttgg tcactttttg ggttgaatgt acgtaaaaat ccccagcagt
     3781 gaaatttgag cgcgtaagca caattctgag gggctttttg tctgtaggtt aggcagtgta
     3841 actaacaata caagaagtta gctctgacta actatttaat taactt
//
LOCUS       DAZFAB010000214         3778 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZFAB010000214 DAZFAB010000000
VERSION     DAZFAB010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.215"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            152..2431
                     /gene="ydeP"
                     /locus_tag="ACHJWA_004764"
     CDS             152..2431
                     /gene="ydeP"
                     /locus_tag="ACHJWA_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFO4311186.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAQNTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSVEFLARLGERYGFTPPHVPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQATLPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2679..2876
                     /gene="safA"
                     /locus_tag="ACHJWA_004765"
     CDS             2679..2876
                     /gene="safA"
                     /locus_tag="ACHJWA_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFO4311187.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVILLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIVNSLQD"
     gene            2950..3711
                     /gene="ydeO"
                     /locus_tag="ACHJWA_004766"
     CDS             2950..3711
                     /gene="ydeO"
                     /locus_tag="ACHJWA_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFO4311188.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTMPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFATCKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMNALAI"
BASE COUNT         1069 a          827 c          865 g         1017 t
ORIGIN      
        1 tttaatctat tgtttaacga taatagatta aaaatagaaa gagagtagtc ccctactctt
       61 atatatccat gttggcgata atccctttgt atgtactgtg catcatcgct attacaaatc
      121 ctaataattc atttccacac aggataagta gatgaagaaa aaaattgaat cctaccaggg
      181 tgctgccggt ggttggggtg ctgttaaatc cgtagcgaat gcagtacgta agcagatgga
      241 tatacgccag gatgttattg ccatgtttga catgaataag ccagagggct ttgactgtcc
      301 gggttgtgca tggccagatc ctaagcacag tgcgtcattc gacatttgtg aaaacggcgc
      361 aaaagcaatc gcctgggaag tcacggataa gcaggtaaat gcctctttct ttgctcagaa
      421 tacggttcaa tcattactta cctggggaga tcacgagctt gaggctgcgg gacgtctcac
      481 tcagcctttg aaatatgatg ctgtcagcga ctgttacaag ccattaagct ggcaacaagc
      541 tttcgacgaa attggcgcac gccttcaaag ctatagtgat cccaatcagg ttgaattcta
      601 tacttcgggc cgcacttcca atgaagctgc ctttctttat cagctttttg cccgtgaata
      661 cgggagcaat aactttcccg actgctccaa catgtgccat gaaccgacaa gcgtgggttt
      721 ggcagcgagt atcggtgtag gtaaagggac cgtgttgctg gaagactttg agaagtgcga
      781 tttagtcatt tgcattgggc ataaccctgg tacaaaccac cctcgcatgc tgacttcgtt
      841 gcgcgcttta gtgaaacggg gagcgaaaat gatcgccatc aatcctctac aggaacgtgg
      901 cctggagcga tttaccgcac cgcaaaaccc gtttgaaatg ctgacgaact ctgagactca
      961 gttggccagt gcctactata acgtgcgcat tggtggtgat atggcgttgc tcaaggggat
     1021 gatgcgcctg ttaattgagc gcgatgatgc tgcaagcgcc gcaggtcggc cctcattgct
     1081 ggatgacgaa tttattcaaa cgcataccgt cggctttgac gagctacgcc gtgacgtgct
     1141 caattccgag tggaaagata tcgaacgtat ttctggacta agtcagacac aaatcgccga
     1201 actggctgat gcatatgccg ctgccgaacg aaccattatc tgttacggaa tggggatcac
     1261 tcagcacgaa catggtaccc agaatgtaca gcaactggtc aatctgctgt tgatgaaagg
     1321 taacattggc aagcctggtg cgggtatctg cccactacgt ggccactcta atgtacaggg
     1381 cgaccgaacc gtcggtatca ccgagaaacc gtctgtagag tttctggctc gcctgggtga
     1441 gcgctatggc ttcaccccac ctcatgtacc tggacatgct gcaattgcca gcatgcaagc
     1501 aatatgtacg gggcaggctc gagcattgat ctgcatgggg ggcaactttg cactggcaat
     1561 gccagatcgg gaagcgagcg ctgtaccgtt aacgcaatta gatttggcgg tacacgtagc
     1621 cactaagctt aaccgctctc atctgctgac cgcacggcat agctatattc tgccggttct
     1681 gggacgtagc gagattgaca tgcaaaaaag cggtgcgcag gcggtaaccg ttgaggattc
     1741 aatgtcgatg attcatgcct cgcgtggcgt gttaaaaccc gccggtgtaa tgctgaaatc
     1801 agagtgtgct gtggtcgcgg gaatcgcgca ggcaacacta ccccagagcg tggtagcctg
     1861 ggagtatctg gtggaagatt atgatcgcat tcgcaatgac attgaagctg tgctgccaga
     1921 gttcgccgac tataaccaac gcatccgtca tcccggtggt tttcacctga taaatgcagc
     1981 tgctgaaagg cgctggatga cgccgtcagg taaggctaat ttcattacca gcaaagggct
     2041 gttagaagat ccctcttcag cgtttaacag taagctggtc atggcgacag tacgcagcca
     2101 cgatcagtac aacacgacga tttatggtat ggatgatcgc tatcgagggg tattcggtca
     2161 acgagatgtg gtctttatga gtgctaaaca agctaaaatt tgccgtgtaa aaaacggcga
     2221 aagagttaat cttattgcgc ttacgccaga cggtaagcgc agctcacgcc gcatggatag
     2281 attaaaagtg gtcatttacc ctatggctga ccgctcactg gtgacctatt ttccagaatc
     2341 gaatcacatg ctaacacttg ataaccacga tccattaagt ggcattcctg gctataaaag
     2401 tattccggtt gaattagaac catcaaatta atgcctcttc tcatttcttc tgctgtcatc
     2461 cgcacagcag aagaattcct cattgattat tatcgcgcaa tttgctcaca tggattaaat
     2521 taaactacat actataagat ataaacttct gcctacagct gtaagaaact ccgctcagta
     2581 ctgaagcacc agtcctattt cctcttttct caagcctgtt atattaagca tactgattaa
     2641 cgatttttaa cgttatccgc taaataaaca tatttgaaat gcatgcgacc acagtgaaaa
     2701 acaaaatcac gcaaagagac aactataaag aaatcatgtc tgtaattgtg gttatcttat
     2761 tactgaccct tacgttgata gccatttttt cggcaattga tcagctgggt atttcagaaa
     2821 tgggtcgcat tgcaagagat cttacacatt tcattgtcaa tagtttgcaa gactgaaaat
     2881 aaacagcaaa ttataaatat gaaatgttaa aaaagtatcg ataaaacttt attgttttaa
     2941 ggagataaaa tgtcgctcgt ttgttctgtt atatttattc atcatgcctt caacgctaac
     3001 attttagata aagattacgc cttctctgac ggcgagatcc tgatggtaga taacgctgtt
     3061 cgtacgcatt ttgaacctta tgagcggcat tttaaagaaa tcggatttaa tgaaaatacc
     3121 attaaaaaat atctacaatg cactaacatc cagacagtga cgatgcctgt tcctgcgaag
     3181 tttttacgtg cttcgaatgt accgactgga ttgcttaatg aaatgattgc ttatctcaac
     3241 tcggaagaac gcaatcatca taatttttca gaacttttgc ttttttcttg cctgtctatt
     3301 tttgccacat gcaaaggttt cattacacta ttaactaacg gtgtgctatc cgtttcgggg
     3361 aaagtgagaa atattgtcaa catgaagctg gcgcacccat ggaagctgaa agatatttgt
     3421 gactgcctgt acatcagtga aagcctgttg aagaaaaaac taaagcaaga gcaaacgaca
     3481 ttctcacaga ttcttttaga tgccagaatg cagcacgcaa aaaatttgat acgcgtagaa
     3541 ggttcagtca ataaaattgc cgaacaatgt ggttatgcca gtacatctta ttttatttat
     3601 gcgttccgca aacatttcgg caacagtcca aagagagttt ctaaggagta ccgttgtcaa
     3661 aggcacacgg gtatgaatac gggcaacacg atgaatgctt tagctatttg attatttgct
     3721 aacgagtagt caaccacgca ccctacgtaa aaattaaaaa tggggcagtc attccctg
//
LOCUS       DAZFAB010000215         3730 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZFAB010000215 DAZFAB010000000
VERSION     DAZFAB010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.216"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(98..1255)
                     /gene="intS"
                     /locus_tag="ACHJWA_004767"
     CDS             complement(98..1255)
                     /gene="intS"
                     /locus_tag="ACHJWA_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HFO4311189.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKRQVWSAAYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLAEAMKGYRKKNFPFLPADQIPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMQWKNVDFENRIITIEASVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKSICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDGKVE"
     gene            complement(1417..1491)
                     /locus_tag="ACHJWA_004768"
     tRNA            complement(1417..1491)
                     /locus_tag="ACHJWA_004768"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1456..1458),aa:Arg,seq:cct)
     gene            complement(1567..2499)
                     /gene="yfdC"
                     /locus_tag="ACHJWA_004769"
     CDS             complement(1567..2499)
                     /gene="yfdC"
                     /locus_tag="ACHJWA_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFO4311190.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            2793..3548
                     /gene="mlaA"
                     /locus_tag="ACHJWA_004770"
     CDS             2793..3548
                     /gene="mlaA"
                     /locus_tag="ACHJWA_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFO4311191.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT          904 a          902 c          877 g         1047 t
ORIGIN      
        1 ttattgttga tggtttcttg gtgtaagaaa tcctgagaag tgaaaagctt tgatatatgt
       61 gcaaggcttt tcttttttat aagaatcgtt gtaataacta ctccaccttc ccatcaagcc
      121 agtccgccca ccactgcatc atttctctgc gcttatcgag atactgagca tggttgtaaa
      181 tcccacgcac agatccgccg ttggcatgtg ctagttgcac ttcaatagcg tcagcaggcc
      241 attcgtgctc gttcataatc gtgctgaatt catgcctgaa tccgtgaccg ctttccagac
      301 cttcatagcc gatttgtttg atcacaagca gtacagcgtt ctcacagatt gacttcttct
      361 tatcgttgcg cccggcaaaa acaaactctg atactggttt agtgatggag cttagcgtag
      421 tgagaagttc aacaacctgg tccgacatcg gaaccacatg aatcttgcgt cccttcatca
      481 cactggcctc gatggtgata atcctgtttt caaaatcgac gttcttccat tgcatggaac
      541 gaagctcttt cgttcttagt gctgtgtagc gtaaaacttt ggtcgcaatg agagatacga
      601 tacttcctga aaatgttgca agtgctttgt tgaatgccgg gatctggtcg gcaggtaaaa
      661 acgggaagtt cttcttgcgg tatcccttca tggcttcagc aaggtcaggt gccgggttat
      721 atttagccct tccggtgaca atagcgtaac ggaaaacctc gccgcatctt ctgcgtgctt
      781 tgttggctcg ctccattgcc ccgcgatctt caaatctgcg gattacttcc agcagttgca
      841 tcggctcaat atcctgaatt tcaaggccgc cgatgatagg taaaatgtcg tcatcaaaca
      901 tttttgcaag ttcagtcgca taggcggctg accagacttg cctcttatgc tcgtaccatt
      961 ccttgtaaat cgcactaaag gaattgttgt tagacgaagc ctttttcgcc tttaccggat
     1021 cgatgccaac cgagatgtct ttcctcgcag tccatgcttt atcccttgcc tcttgcaaag
     1081 tcataagcgg atattttccg acggtcagga ttttctcctt accgtcaatc ttgtagcgaa
     1141 gctgccatac ctttttccct gatacaggga cataaaggta caggccatta ccatcgagta
     1201 ggcggtatgg tttttctttc ggctttgctg cttcaatctg cttaacggtg agcatgggta
     1261 aaaatccggt gggtaaaatt attttatcca ctttttaccc gtcatggcgt gcggctgtca
     1321 acgatctgac gcgaaccatg acgaactgtg aatctacgga aggcttgata ttcaggggat
     1381 tttgcggact ggtacggatg gaggcgaact gataaatggt gtcccctgca ggaatcgaac
     1441 ctgcaattag cccttaggag gggctcgtta tatccattta actaagagga caatgcggca
     1501 tgagtatacc cgctaatgga ctgcggggta agtacgctgc cgctcgattg cttaaaccct
     1561 cgccatttat gccgggtttt tatcattttt cttaatgttt tccgcacgtt ctgctttttg
     1621 gcgtgcttct gctttgcgct tgttgctcat gtcgttacgg atctgtgcat gactcattaa
     1681 cgcgaagata aaggtgccgc cgcagatgtt ccccgctaaa gtaggtagtg cgaagggcca
     1741 gatgaaatcg ctccagtgca gcgtaccgtt aaacaccaga tagaggattt caacagaacc
     1801 gacaacgatg tgagtggtgt cacccagggc aataagccag gtcatcaata taatcaccac
     1861 aatctttgcc gcacccgctg caggaaacat ccataccata gtggcgatca gccagccgga
     1921 aatgatcgcg ttggcaaaca tctcgctggg ggtgttcttc atcacatcca tgccgatttt
     1981 gacaaatgca tcgcgagttt cttcattgaa gattggcata tattcaaatg cccacgccgc
     2041 aatacctgtc ccgagaatat tacccagcag cacgacgccc cataaccgca taagtaagcc
     2101 aacgttgctc attgtcggtt tttgcatgac gggtagtacc gcagtcacgg tgttttcggt
     2161 aaataattgc tggcgggcca taatgacgat aataaaacca aaggtataac cgagattctc
     2221 cagcaagaag ctgcccggca caccttccag ttcgacatga aatatccctt ttgccagtaa
     2281 tgaagcgccc atcgacagac ccgccgcaat ggctgaccac agtagtgcca ttgcgtcgcg
     2341 ttccagctct ttttcaccat cctggcggat atgctcatga attgccatcg cccgggaggg
     2401 gagtcgatct tcatctattt ctattttttt gccacgctct ttttcttcgc tctcaacttc
     2461 aatttcgtcg ctgtgttgat caattttgtc gttgtccatg gtctcttctc ttgaattagc
     2521 acgtatagct aaagcgtagc ggcttttttg ctcgcaactg gcgggagtta ctttgaaaat
     2581 gtagaaaagg ccgcgtttgc ctttttctgt ttctatagaa tcaagtagcc tacagggcgg
     2641 cgattaccag gctatgatca aatcagcaaa tcagggcgtc tggacatcag ttgacgtgct
     2701 gttacaatcg cccacaccta aacaggcgga tacggtatcg ttccgtcatg gatggcaaac
     2761 tgcataagcc ataaaaaaac agggagacat ttatgaagct tcgcctgtcg gcgcttgctc
     2821 tgggaactac gcttctggtg gggtgtgcga gttccggtac agatcagcaa gggcgttctg
     2881 acccgttaga agggttcaac cgcaccatgt acaactttaa cttcaatgta ttagacccgt
     2941 atattgttcg accggtggct gtcgcctggc gtgattatgt tccgcaaccg gcgcgtaacg
     3001 gtttgagcaa ttttaccggc aaccttgaag aacctgcggt gatggttaac tacttcttgc
     3061 agggcgaccc ttatcagggg atggtccact ttacccgctt tttcctgaac accattttgg
     3121 ggatgggcgg ctttattgat gttgcaggga tggcgaaccc gaaactgcaa cggactgaac
     3181 ctcaccgctt cggtagtacg cttggtcatt atggcgtggg ttatggcccg tacgttcaat
     3241 taccgttcta cggtagcttc acgctgcgtg atgacggtgg tgatatggcg gatggtcttt
     3301 acccggttct ttcctggctg acctggccga tgtctgtggg taaatggacg cttgaaggta
     3361 tcgaaacccg cgcacagctg ctggattccg acggtctgct gcgtcagtcg tccgatcctt
     3421 atattatggt gcgcgaagcg tacttccagc gtcatgattt catcgctaat ggcggcgaac
     3481 tcaaaccgca ggaaaatccg aacgcgcaag cgattcagga tgatttaaaa gatatcgatt
     3541 ctgaataaga aacaaataaa aaaggtgagt cgcaaaactc accttttttg ttgttatccc
     3601 tcaattacca caagtaaaag gagataaacc gacaatgacc cggacgtaat tatgtaaaaa
     3661 tactgatgtt cgtcactgac tatttccgat attcagctgt tgaaaaatca aaaaaatgga
     3721 aaaataattt
//
LOCUS       DAZFAB010000216         3699 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZFAB010000216 DAZFAB010000000
VERSION     DAZFAB010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3699
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.217"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..703
                     /locus_tag="ACHJWA_004771"
     CDS             <1..703
                     /locus_tag="ACHJWA_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFO4311192.1"
                     /translation="VWFLNVITSVPPTTARALIQGLKHDLLADDTALRALIPQRLIAF
                     DDAVRRTLKEEEKLVNSSDWGYDTQAFARWRPEYGYFAKQAGFTVKTSASLAALWQVV
                     NQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVDSWKVIVVEPEK
                     QLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWLLMIPAHLFIFR
                     GMAKRIARLAEQSTD"
     gene            802..1815
                     /gene="ybjS"
                     /locus_tag="ACHJWA_004772"
     CDS             802..1815
                     /gene="ybjS"
                     /locus_tag="ACHJWA_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO4311193.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(1812..2642)
                     /gene="amiD"
                     /locus_tag="ACHJWA_004773"
     CDS             complement(1812..2642)
                     /gene="amiD"
                     /locus_tag="ACHJWA_004773"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFO4311194.1"
                     /translation="MRRVFWLIAVALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(2639..2962)
                     /gene="ybjQ"
                     /locus_tag="ACHJWA_004774"
     CDS             complement(2639..2962)
                     /gene="ybjQ"
                     /locus_tag="ACHJWA_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFO4311195.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            complement(3173..>3699)
                     /locus_tag="ACHJWA_004775"
     CDS             complement(3173..>3699)
                     /locus_tag="ACHJWA_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFO4311196.1"
                     /translation="SVKINNVNTHSLKFINCHFFNVDMIRVNFSKCLFYDCSMHGVKI
                     KPWLPVKWTKELINDYLYGCLLSLYSICARDIYNMNAGNNVKVAADAFLEILYSLKNK
                     YCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRYIPQAQDGED
                     FRNHLQYKLTLPTH"
BASE COUNT          907 a          968 c          902 g          922 t
ORIGIN      
        1 ggtgtggttt ctcaatgtga ttacttccgt accgcccacc accgccaggg cgttgattca
       61 ggggctgaaa cacgatctgc tggcggatga caccgcgcta cgtgcgctca tcccacaacg
      121 gctgatcgct ttcgatgacg cggtacgtcg caccctgaaa gaagaagaaa agctggtcaa
      181 ctccagcgac tggggatacg acactcaggc ctttgcccgc tggcgaccag agtatggtta
      241 ttttgccaaa caggcgggat ttaccgttaa aacgtccgcc agccttgcgg ctttatggca
      301 ggtggtgaac caaatcggcg gtaaagagcg ttatttcttt ggcaatattt tgtggcagac
      361 acgggcgttg atggaccgtg cgatcggtca taaattagcg aaaggccgtc cggagcgcga
      421 atatttgcaa actggcgatg cggtggatag ctggaaagtg attgtcgttg aaccggaaaa
      481 acaacttacg ttgttatttg gcatgaaagc accggggctg ggacgactgt gttttaccct
      541 ggaagataaa ggcgactatc gtactatcga tgtccgcgct ttctggcatc cgcacggtat
      601 gccggggctg ttttactggt tattgatgat ccccgcgcat ctgtttattt ttcgcggaat
      661 ggcaaaacga atcgccagac tggcagaaca aagcacagat taatcgacaa aacctgcgtt
      721 attctttcat gtttcccatt gcatcaaggg ggaaatcacg gaagaatgcg cacgtcattt
      781 tctcaacaac agtggattga tatgaaggta ctggttaccg gcgccaccag cggcttaggt
      841 cgaaacgcgg tagagttttt atgccagaaa ggcatcagcg tgcgagcgac cggtcgcaac
      901 gaggcaatgg gtaaattgct ggaaaaaatg ggcgcagagt ttgttccggc ggatctgacc
      961 gagctggtct catcacaagc taaagtgatg ctcgcgggca ttgatacgct gtggcactgc
     1021 tccagcttta cctctccctg ggggacacaa caggctttcg atctggctaa cgttcgcgcc
     1081 actcgccgcc taggtgaatg ggctgtcgcc tggggtgtac gtaactttat tcatatctct
     1141 tctccctccc tgtacttcga ttatcaccac catcgcgata ttaaagaaga ttttcgccct
     1201 caccgcttcg ccaacgagtt tgcccgcagc aaagcggcca gcgaagaagt gatcaatatg
     1261 ctttcgcagg cgaatccaca aacgcgcttt actattctgc gcccacaaag tctgttcgga
     1321 ccgcacgata aagtctttat cccccgtctt gcgcatatga tgcaccacta cggcagcatt
     1381 ctgttaccgc atggcggcag tgcgctggtg gatatgacct actatgaaaa tgccgtgcac
     1441 gccatgtggc tggcaagcca ggaagcctgc gataagctac cttccgggcg tgtgtacaac
     1501 atcaccaacg gcgagcatcg cacactgcgc agcatcgtgc agaagctgat cgacgagttg
     1561 aatattgact gtcgtattcg ttccgtccct tacccgatgc tggatatgat cgcccgcagc
     1621 atggagcgtt taggccgcaa gtcagcaaaa gagccgccgc tgacccacta cggcgtctcg
     1681 aagcttaatt ttgactttac gctggatatt acgcgggcac aggaagagtt aggttatcag
     1741 ccggtcatca ccctggatga aggtatcgag aaaactgccg cctggctgcg cgaccacgga
     1801 aaactgccgc gctaatcctg cccgtatttc tccagcaacg cttcggcaat cgcctgagtt
     1861 tctgcatccg cttcgccgtt atataacgtc gggcggaaat gcatctggaa cgccataatc
     1921 acgcgccgct gctcgcgcgg tgtcatatca ggtttaacgt cataaccgta gcgcgccaaa
     1981 agctccagca atgacgcggt atctaccggt gtgtgcggcg cgcgcccggc aaggtaaaag
     2041 ttcacccgct gcgcatccgg ccaggcacca atcccctgct gcgccagttg ctgccaggga
     2101 aataatggcc ccggatcgtc tttgcgctgc ggggcgatat ccgcatgtgc cactacgttt
     2161 tccggcttga tgtgataacg ggcaataata tctttcgcca gcggaataag cgcctgaatc
     2221 tgtgccggtt caaacggggc aaaatatttc acccccgccg atttttgcca gccacggttt
     2281 tccagctcaa tgccaataga ggtgtcgtta aggcgcgttg ccccgcgcca ggcgctaatc
     2341 cccgcatgcc aggccagttc ttgttccggc accaattgcc agatgcgcgg tttaccgttg
     2401 tatcgcggtg gtaccgcagg gaccagataa tgcgagctga cctgcttatc ggtcagtgtc
     2461 gccagcgagc tatcaaaatc atctgcggtg tagtggatca ccagcacttt aatgcgcgga
     2521 tacgccgcct gcgcctggcg tcgggtatca agctgatatc cctctttctc gacaatgcct
     2581 ttttcgcctg cgcaccctgc cagtaacaga gcgacagcga tcagccagaa gactcttctc
     2641 atcgacgcgt tttcaccgcc gtaccgctga cgctaaccat cagcatactg ccgttttgcc
     2701 cgaccgtttc gtagtcgata tcaataccga ctacggcatc ggcccccagc gcccgcgcct
     2761 gggagccaag ctcctcaaag gcgatctccc gcgctttacg tagctctttt tcgtaggcac
     2821 cggagcgtcc gccaacgata tcgcgaatac cagcaaagaa atcacggaaa atattcgcgc
     2881 ctaaaatcgc ttcgccagtc accacaccgc aatattcaac gatggtctgg ccttccagag
     2941 ttggagttgt ggaaaattgc atgatcgatc tccttgtttg ttaagccttc gatctctaaa
     3001 gcattatcgg actgatatgc catgttggga agaaggtttt gatcagttta ggaaatcatc
     3061 tcattgtgtc aaaaacagct acataagcct gtacaattat ctgtttcgta accggatatt
     3121 ggtttcatta tggagcaccg cttacctgcc ctattatgtt gtgccggcaa cattaatgag
     3181 tgggaagagt caacttatat tgtaaatgat tcctaaagtc ttccccatct tgcgcctgag
     3241 gtatataccg ctggattgct ttttggtccg ccgccgcaaa acaagaaagt aataagatct
     3301 caatgctttt gtcattaata taagcgaaga tcattcttgc aaattcgtat ataaacgccc
     3361 gatcctgagc gcttaatagt ttaatacaat atttattttt aagagaataa agtatttcaa
     3421 gaaaagcatc cgcagcaacc ttcacgttat tcccggcgtt catattatat atatctcttg
     3481 cacagataga gtacagagag agtaaacagc catacaaata atcatttatt aactcttttg
     3541 tccattttac tggcaaccat ggttttatct tgacaccatg catgctacag tcataaaaaa
     3601 gacatttgct gaaattaact cttatcatat caacattaaa aaaatgacag ttaataaatt
     3661 ttaaagaatg agtatttacg ttgttaattt taacagatc
//
LOCUS       DAZFAB010000217         3676 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZFAB010000217 DAZFAB010000000
VERSION     DAZFAB010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.218"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(53..691)
                     /locus_tag="ACHJWA_004776"
     CDS             complement(53..691)
                     /locus_tag="ACHJWA_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFO4311197.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSVAGQLVARAVFLSPPYSVAEEELSVLLENIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(704..2806)
                     /locus_tag="ACHJWA_004777"
     CDS             complement(704..2806)
                     /locus_tag="ACHJWA_004777"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFO4311198.1"
                     /translation="MTNGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAECWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            complement(2827..3453)
                     /locus_tag="ACHJWA_004778"
     CDS             complement(2827..3453)
                     /locus_tag="ACHJWA_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFO4311199.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
BASE COUNT          924 a          969 c          798 g          985 t
ORIGIN      
        1 ctaatgcagt ggctgcaact gtccacccta tccatgacat ctccctgaaa tttcaggggt
       61 tctgaaactg ctctacctcg aaccattcgc ataagccata agccttgcca taggtcataa
      121 atcgctcgct aaacaagtac aacaccgccg tcgatgactc tacctgccgt atatcggcat
      181 attcaggtgc aacatccatt gcggcgatcg cggcttcaat ctgcgcttcc tggaagtaat
      241 atggcgcttg catcagcatg gcgactttgt aaggacgagg atacgtctgg cactcaaaac
      301 gaacggcgtg tgcaatggcg cgacaaatgt cctgctccac aacttgcaaa gacatcgccg
      361 cgtagttttc actcatggcc tgcgttgagt aatagtaatc atcctgactt cccgtcatac
      421 aagcgatatc cgcgtagtct ccgttctgct tgatgttttc cagcaaaact gacagttcct
      481 cttctgcaac tgaatagggt ggcgagagaa acacggcgcg cgccaccagc tgcccagcca
      541 cagaatgctg gcgaatgaac tctgccagga tttcagccgg ttctggttcg gttaccgtat
      601 caacggggtg acgcatcccc tgcaaccatt gccgcttcgc ctcgtcaact tcgctgactt
      661 tcgctaacgg caattcatcc tgatggttca tcttacccat tccttacgca ggcagtagat
      721 tgtgtgctgc cagatcggcg gcaatatctt ccagccccag acgctgcaat gtttcgcggg
      781 tcggacaacc aagctgtggg tcccagccca tttctttgta gaacatcgtg agcgaggcat
      841 gcatatcatc acgatccatt ttgtcagtac cttcagtaaa gaccgggatc tgcggatcct
      901 tgtcgaatac ccaggaacag ataagatcgt gttcattacg catatctttg gtttgcatca
      961 gtttcaccgt gtaggcacga tgcaatgtaa agatacgctc tgcagctaaa tctaattttt
     1021 cctgagtcat ctcttcgcca gtgatcgctt tgaagaattt ggcttcaagc gccagatcgc
     1081 cccggtaatt acggcttttc agcggcgaaa cggtcattgg ccagacccag ttgcacaggg
     1141 tgacggcgtt atgcaaacag acccgcaaca gcgaccactt agcatattta atttttgcgt
     1201 cattgattgg cgtgtagttt ttggtttcat cgtaagcatc ttcagaacca aaaagttctt
     1261 tcgccacttc acgttgcagt ttcaatggca agccggaacc aataaagttg atatgggtat
     1321 gcgtcatgca gtcacggttg aacatacagt taacaatgga accgacctgc gctgacgctt
     1381 cattggcatg gtgaaccgga tagccaaacg gcgaccagag tttatttttc gcgtagcccc
     1441 agtactcttc acccaaattc cagcattctg cgatggcata tgaaccatca gccaggtgac
     1501 tcagctcacc cacacgatgc gccagacggt agtaaaaatc tttaatgaag ttaacgtctc
     1561 ccgcttccag ttgatcccag cgaatttctg catactcttc agcaggcaga acacgcttga
     1621 acacaccttt gctgtaacaa taagtaaagt cgcgatgcaa ctgcccgtag ttacaccata
     1681 ggccgtagtc atcgaacaaa ttcagaccaa ccaggttacc aatcacacga ccatcgtctt
     1741 tatcttcaaa atctttcggc ccgttcggga agatggtggt atggacaaag tttgctacac
     1801 aagtgttacc acctgtgctg ggcacgccaa actctttcac ccgaggaata ttcatttggg
     1861 tcatacaacg gatcgggcaa gagtggcagc cgctcatttt tactgtgtat ttctctgctg
     1921 ccggacctaa gtcaaaaacg gatttatagg tacgaaagcc gaccgtattc tgattgcctg
     1981 gcggaatttc acccgtttca attggaccgc cttcagccgc gccccaaaac agccctttac
     2041 gtgctgtcca gcgtgacttg ggatctgaat actctgccca cgattgcggc gtacttggca
     2101 cgacatggtt gttattcgca ccgataagtt cagtcatcat gtaatcattg agacgcttca
     2161 tctcctgacg atcggcaatg ttgacccctt tcgtcccttc aaccgcaatc gctttcaggt
     2221 ttttcgaacc cattattgcg ccagttcccg caccgccgct gtggttacgg ctattcaaca
     2281 tgccagagag aggaacaagg ttttccccag cctgaccaat agccgccaca caggtttccg
     2341 gactggtcaa gcgacaaatt tcttccgtcg ttgcgcgcgt cccttttccc cataagaaat
     2401 ctgctttttc caggctaact ttgtcatctt taatcttcag ccataccggt gatttcgcct
     2461 tcccttcgat aataatgacg tcgtatccag cgaatttcat ttgcgctgca aaaaagccac
     2521 ccatatgggc atcgacgact aaatttcctt tggtaaaagt agaaagtgag gtgatattta
     2581 cgcgagaact acagggggca ccagatccag ttaatgggcc ggtagcaaag actaatttat
     2641 tcgcttcatc gaaaggtttc gtgcctggcg gtacttcgtc atacataatt ttgtagccga
     2701 agcccatgcc accgacaaaa cttttaaact tactggaatc ttcgagggta atatttcctg
     2761 tcgtgagatt gactcttaat atattacctg tccaaccgtt agtcatgatt ttttcctttg
     2821 caagattcac acagtaatat ctttccactc gataattttt aacgcccctg ttgggcaggc
     2881 gtttgcgcat tcaccgcata acacacattt cgaggatttt ttactttcgg tatttacggt
     2941 ggccatcatc cacgggcatg ccgtagtaca ggcgctacag ccaatacaac gtttatgatc
     3001 gacggtaata cagccttctt tctgctgcca ggtaatcgca ccaatcgggc agacgttcat
     3061 gcattgcggc tctttgcatt gacggcaggt gtccgcggta tagttgagat cgccatacag
     3121 gccgccgcca gagccaaccc cgttgtcgcc aaagaaataa ttgcgatgga ttttgatacg
     3181 ggagaagaat gtccctactg agccatcgtt gaagttggta caagagattt cacagcggtg
     3241 gcaacccgtg catcgcgctc gctgggtcac cagcacccct ttgggtgtat ttatcaaccc
     3301 tactgtgccg ctatcaatat cttcctgttt gcaacccaaa agtgacagta acgcaggggc
     3361 aattgttaga ccagcaaggc cttttcctga tatacgcaag aattctaatc gcgttaagcc
     3421 aatatctaat agtggacgat caaccgggtt catttatttc gcatcctctt cgcaaacaca
     3481 gtgctgacaa ttcagcagcc tgatattaag cattcaacta atacgctgct gactctgtcc
     3541 ccaacaccag atattttccc taacccgata gtgtatgaga aaatatttaa cgccgataaa
     3601 ctaatccttt atggttagga gaaaaaataa tgtggttatt ctgggctctt aacattgatc
     3661 attatcaatt aaaatt
//
LOCUS       DAZFAB010000218         3644 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZFAB010000218 DAZFAB010000000
VERSION     DAZFAB010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3644
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.219"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..500
                     /locus_tag="ACHJWA_004779"
     CDS             <1..500
                     /locus_tag="ACHJWA_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HFO4311200.1"
                     /translation="LRYITVQRISEVIQRLIQERISVRNMRLVMEALALWSPREKDII
                     TLVEHVRGALGRYICHKFSYSGEIKAIVISPEIEDRIRDGVRPTAGGTFLNLDASEAE
                     MILDNFKLALSGINIPIKDIILLGSVDIRRFIKKLIESSYRDLEVLSYGELTENVPVN
                     ILKTI"
     gene            532..1824
                     /gene="sctN"
                     /locus_tag="ACHJWA_004780"
     CDS             532..1824
                     /gene="sctN"
                     /locus_tag="ACHJWA_004780"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SctN"
                     /protein_id="HFO4311201.1"
                     /translation="MKKLKLLNKYSYLHSINGSLIEAELDDVSVGEVCEIYASRQANE
                     RIARAQVVGFRNGKTLLNLIGSSVGLTRTAVLKPTGEQLTIQISDAFLGSVLNASGQI
                     MERFVPDTPGDRGNLRLIDELPPSYQERRVINTPLETRIRVIDGVLTCGIGQRVGIFA
                     SAGCGKTVLMHMLVNNTEADVFVIGLIGERGREVTECAESLKKSVNAAKCVLVYATSD
                     FSSVDRCNAALMATTVAEYFRDRGKRVVLFIDSMTRYARALRDMKLAAGEPPARRGYP
                     ASVFDSLPRLLERPGPTLKGSITEFYTVLLEGEDESDPLGDEIRSILDGHIYLSRKLA
                     GQGHYPAIDVLKSVSRVFGQVTDEKHRDNAARVRKNLTTLEDLQVFIDLGEYRAGQNA
                     ENDFAMNARPKLTNWLKQSVNEKMPMSETLKELERIVK"
     gene            1814..2272
                     /locus_tag="ACHJWA_004781"
     CDS             1814..2272
                     /locus_tag="ACHJWA_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
                     /protein_id="HFO4311202.1"
                     /translation="MLSKVNRLIRRTAQSLAACEASLQKLNAEKEKLAEKERLYDMQL
                     KNLQSLLDMKELLGEVVFRQDIFYSLRKVTVIQQQIAEINLEKQKIAERRKILNKEIV
                     QQQAQRKHWWLKGEKYDRLKKRIKKQLLNQMLYQDELEQEEKYNGRSQEN"
     gene            2250..2483
                     /locus_tag="ACHJWA_004782"
     CDS             2250..2483
                     /locus_tag="ACHJWA_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311203.1"
                     /translation="MDEVKKIEHCKILNTDTPDNSASDLDSFLKKNKKNKEMSEIVAG
                     TILQLLRQTQDNQFSTLGKKNGHMKSIDNKSSL"
     gene            2556..3125
                     /locus_tag="ACHJWA_004783"
     CDS             2556..3125
                     /locus_tag="ACHJWA_004783"
                     /inference="COORDINATES: protein motif:HMM:NF014559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     determinant, SpaN/EivJ family"
                     /protein_id="HFO4311204.1"
                     /translation="MTSRDQGINSELSNRTIQFKEKIHNGIHTEYITDQKHSNNKDRE
                     KKYRDGDKINGPQAHSLDITNERRFADNRTMFTQHIEKQRNVNTLNQNDINNSANNAN
                     VRENELTYQFQRWGQNHTVRILESSEGIRLKPSDTLVSDRLHEAQHNDVTAQRWVLTE
                     QDERQGQRHQPHEEQENEGKFENDQKDES"
     gene            3085..>3644
                     /locus_tag="ACHJWA_004784"
     CDS             3085..>3644
                     /locus_tag="ACHJWA_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
                     /protein_id="HFO4311205.1"
                     /translation="MKANSKTIRRMKVNVRSEESKSKHSTFETTFQNWKENGEDVALL
                     MPEFSAKWLPIAEESGSWSGWVLLREIFPLISAELAGMALMPETERLIGEWLSLSSSP
                     LNLKYPELKYNRLCVGKVFDGVLSPAQPLIRIWTGELNLWLDKVTVCQYENAPTLDKK
                     SLYWPIHFVIGFSKTCYRTIVDIEVG"
BASE COUNT         1221 a          654 c          823 g          946 t
ORIGIN      
        1 tattaagata tataaccgtg cagcgcattt cagaggtaat tcagcgtctg atccaggagc
       61 gaatttcagt gcgcaatatg cggttagtta tggaggcctt agcattatgg agcccacgag
      121 agaaagatat aatcacactc gtcgagcatg tacgaggtgc attaggtcgc tacatttgcc
      181 ataagttttc atattctgga gaaattaaag caattgtgat ttcgcctgaa atagaggaca
      241 gaatcagaga tggtgtcagg cctaccgctg gcggtacttt tcttaatctc gatgcatccg
      301 aagctgagat gattctggat aattttaaac tggcactttc tggaatcaat attcctatta
      361 aagacattat tttactggga tctgtggata ttcgtcgttt tatcaaaaaa cttattgaat
      421 ctagttatcg agacctcgaa gtactttcgt acggggaact aacggaaaat gttcccgtta
      481 atattctgaa aactatttag gataatgtct tgcgatatgg agcatgctgg tatgaaaaaa
      541 ttaaaattat taaataaata cagctactta catagcataa acggctctct gattgaagca
      601 gaacttgacg acgtttctgt gggtgaagta tgcgaaattt atgcgagcag gcaagcaaat
      661 gaacgtatag ccagggctca ggtcgttggc tttcgcaatg ggaagacatt attaaacctt
      721 ataggcagct ctgtggggct tacacgtact gcagttttga aaccaacggg agagcagtta
      781 accatacaga tcagtgatgc ctttcttggt tcggttttga atgcctctgg tcaaattatg
      841 gaaaggtttg tcccggacac tccaggcgat aggggaaatt tacgcctgat tgatgaactg
      901 ccaccgtcgt atcaggaacg tcgagttatt aatacaccac ttgagacacg gataagagtc
      961 attgatggcg tactgacatg tggcattggt caacgtgttg ggattttcgc ctcggctggc
     1021 tgtggcaaaa cggttttaat gcatatgctc gtaaacaata ctgaagctga tgtgtttgtt
     1081 attggcttga ttggcgaacg tggtagggaa gttacggaat gcgcggaatc gctcaaaaaa
     1141 tcagtaaatg cagcgaaatg cgtattggtc tatgccacct ctgatttttc gtcagtagac
     1201 cggtgtaatg ccgccctgat ggcgacaacc gtggcagagt attttcgaga tcgaggaaag
     1261 agagttgtac tttttatcga ctcaatgacg cggtatgcac gagcactacg tgatatgaaa
     1321 ctggcagcgg gtgaaccacc tgccagacgt ggttatcctg cttctgtttt tgacagtttg
     1381 cctcgcttac tggagaggcc gggaccaaca ctaaaaggaa gtattaccga gttttatacc
     1441 gtgttactgg aaggggaaga tgaatcagac ccactgggtg acgaaattcg ctcaatcctt
     1501 gatggccata tctatctaag tcgcaaactc gcgggtcaag gtcactatcc ggcaatagat
     1561 gttctaaaaa gtgtgagtcg agtatttggt caggtcactg atgaaaaaca cagagataat
     1621 gctgcacggg taaggaagaa cctcacaact ttggaagatc tacaggtgtt tattgactta
     1681 ggggaatatc gcgcagggca aaatgctgaa aatgattttg ctatgaatgc ccggcctaaa
     1741 ctcacaaatt ggttgaaaca gtcagttaat gaaaaaatgc caatgagtga aacgctaaaa
     1801 gagcttgaac gaattgttaa gtaaagttaa tcgacttatc agaagaacag cgcagagcct
     1861 ggctgcatgt gaagcatcat tacaaaagtt aaatgcagaa aaagaaaagt tagccgaaaa
     1921 ggaacgcctg tatgatatgc aacttaaaaa cctgcagtca ctgttagata tgaaggagtt
     1981 actcggcgaa gttgtatttc gccaggatat attttactcg ttacgtaaag ttacagtcat
     2041 tcagcagcag atcgctgaga taaatcttga aaagcaaaaa atagccgagc ggcgaaaaat
     2101 acttaacaag gaaatagttc aacagcaagc acaaagaaaa cactggtggc taaaaggaga
     2161 aaaatacgac cgactaaaaa agcgcatcaa aaaacaactt ttaaatcaga tgctttatca
     2221 agacgagctt gaacaggagg aaaaatataa tggacgaagt caagaaaatt gaacattgta
     2281 aaattttaaa cacagacaca cccgataatt ctgcttcgga tttagacagt ttcttaaaga
     2341 aaaacaaaaa aaataaagaa atgagtgaga ttgtcgctgg aaccattctc cagttattac
     2401 gtcagactca ggataaccaa ttttctacgt tggggaaaaa gaatgggcac atgaagtcta
     2461 ttgacaacaa atcgtctctc taggttgaca ggaatccatc tctttctttg caaacaaaag
     2521 gagccgagca attttctaca aagaaacttc tcaatatgac ttcacgagat cagggtatta
     2581 attctgaact atctaatcga acgatacaat ttaaggaaaa aatccataat ggtatccata
     2641 cagaatacat cacagatcaa aaacacagta ataataaaga tcgtgaaaaa aaataccgcg
     2701 atggagacaa aataaatgga ccgcaggccc attcattaga cattactaac gaaagaaggt
     2761 ttgctgataa cagaaccatg tttactcaac atatcgaaaa gcaaaggaat gttaatacgc
     2821 tcaatcagaa tgatataaat aattctgcta ataatgccaa tgtgcgcgaa aatgaattga
     2881 catatcagtt tcagcgttgg ggacaaaatc atacggtccg gattctggaa agtagtgaag
     2941 ggatacgact aaaaccatca gacacacttg tctccgacag actccatgaa gcccaacaca
     3001 atgatgttac agcacaacgt tgggtattga ctgagcaaga tgaacgacaa ggacaacgac
     3061 atcagcctca tgaggagcaa gaaaatgaag gcaaattcga aaacgatcag aaggatgaaa
     3121 gttaatgttc ggtctgagga aagtaaatcg aaacactcta cgtttgaaac cacttttcaa
     3181 aattggaaag aaaatggcga agatgttgca ttattaatgc ccgaatttag tgctaaatgg
     3241 ctaccgatag cagaagagag tggcagctgg tctggatggg tgttgcttcg agaaatattt
     3301 cccttaattt ctgccgagct cgctgggatg gctttaatgc cagaaacgga aaggttaata
     3361 ggggaatggc tcagtttatc cagttccccg ttaaatctaa agtatcctga actaaaatat
     3421 aatcgtttgt gcgtaggtaa agtatttgat ggagtattga gtccagctca accactgata
     3481 agaatatgga caggggaatt gaatctttgg ttagataaag tcacagtctg ccaatacgaa
     3541 aacgctccaa ctttagacaa aaaatcatta tattggccca ttcattttgt aattggattt
     3601 agtaaaacat gttatagaac tattgttgat attgaagtcg gtga
//
LOCUS       DAZFAB010000219         3457 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZFAB010000219 DAZFAB010000000
VERSION     DAZFAB010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.220"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..613)
                     /gene="espL2"
                     /locus_tag="ACHJWA_004785"
     CDS             complement(<1..613)
                     /gene="espL2"
                     /locus_tag="ACHJWA_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL2"
                     /protein_id="HFO4311206.1"
                     /translation="MPIINKSASNYVEYISKNNPPYLSKKRDASINLNGKVSDCNGEI
                     IWCRHIASYWSEFFCSNSGKIDYETFSSPQLLSKAIVIQENKGTNNIKGDVYFVENES
                     WGSVIYNLFLQLEKENKSHTSLEVHSPGHAMALGIKIKNDKENKFVINFYDPNQTATH
                     KRVFFCTNNICDIINLTAYDFLSEQCLKCYGLKEDTLSLFVDKT"
     gene            complement(604..1167)
                     /locus_tag="ACHJWA_004786"
                     /pseudo
     CDS             complement(604..1167)
                     /locus_tag="ACHJWA_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178393.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            complement(1401..2141)
                     /locus_tag="ACHJWA_004787"
     CDS             complement(1401..2141)
                     /locus_tag="ACHJWA_004787"
                     /inference="COORDINATES: protein motif:HMM:NF033590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFO4311207.1"
                     /translation="MAERLREIQKQVITVCDREADIWHYLHYKVSRGKPHAECDAKRR
                     AGSPPGPDAHQLQRSQHKKSRQQRPGALAHVCLLRGAGEDGACWYPLTSEKVASAADA
                     RRIVSHYEQRWLIEEYHKAWKSGGTCVESLRMQTRDNLERMVVIAVRVLGLRQGGISE
                     ETQNDSCEKILTPTEWKLLWVKLEGKQLPAQTPTLKWACLKLAKLGRWHDSKRTSSPG
                     WVVMWDGWFRHQDMAEGYLVMKSLDQEI"
     gene            2061..2534
                     /locus_tag="ACHJWA_004788"
     CDS             2061..2534
                     /locus_tag="ACHJWA_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001502749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311208.1"
                     /translation="MQVMPDIRFPVTDSDYLFLYLPQPFGHALRSLLPAVTFFFFTGT
                     FFSLVLNTVGVRGTTPALLFDKIDWQGAVGGVHQDRVSQQPVRLRVSGGIPQVIKCRT
                     VTEVNGGGIFQREEQRMCLTCPCSGSKSGFGNGLRGHRRVTYQAVRTFNLSSEVG"
     gene            complement(2466..2669)
                     /locus_tag="ACHJWA_004789"
     CDS             complement(2466..2669)
                     /locus_tag="ACHJWA_004789"
                     /inference="COORDINATES: protein motif:HMM:NF026057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase DNA-binding-containing protein"
                     /protein_id="HFO4311209.1"
                     /translation="MVLSDCYSLANEQSGHARLGDPRRTRRLVSLTSSLAQHAGLSIV
                     KSSHFTAQVEGAYRLIRNPSVSP"
     gene            2847..3020
                     /locus_tag="ACHJWA_004790"
     CDS             2847..3020
                     /locus_tag="ACHJWA_004790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311210.1"
                     /translation="MNKTANVAAHAVVNAALAVVQGNNARASAAGAATGDVVGMIATE
                     MCGISPGTVTCKR"
BASE COUNT          902 a          771 c          787 g          997 t
ORIGIN      
        1 ttgttttatc cacaaaaagt gacagtgtgt cttcttttag cccataacat ttcagacatt
       61 gctcactaag aaaatcatat gctgtcaggt ttattatatc acaaatgtta ttagtgcaaa
      121 aaaatactcg tttatgcgtc gcggtctgat tgggatcata gaaattaata acaaacttat
      181 tttccttgtc gttttttatt ttaatgccca gagccattgc atgtccggga gaatgaactt
      241 ctaatgatgt gtgagattta ttctctttct caagttgaag aaaaaggtta tatataacgc
      301 ttccccaaga ttcattttca acaaaataca catcaccctt aatattattt gtccccttgt
      361 tttcctgaat tacaatagct ttgcttaata attgagggga tgaaaatgtt tcgtagtcta
      421 tctttcctga attactacaa aaaaactcac tccaatatga tgctatatgt cgacaccata
      481 tgatttcccc attgcaatca ctaacctttc catttagatt tatactggca tctctctttt
      541 ttgataaata agggggattg ttttttgaaa tatattcaac ataatttgat gccgatttgt
      601 ttattattgg catatgtttg tatcctttct ataaattttc cgcctgacag tggaaataat
      661 atattctact gatttttgta gtttaaacga tttatgaaga attttactgt tgtacagtac
      721 cagatactca gccagtttca gattgaacat attcagctat tcaaagagca attcttcatt
      781 aaactcaata aattgttccc tcagtgttcg gttaaaatgc tcacttatgg catttatttt
      841 cggcgtatag agataagtcc atacgaaccc gacggtggct tcctgtagcg ttttgtcgaa
      901 ttttcccgag aactctttgc ccatcatcag tgatgatctg gttgatacca acaaggaaca
      961 actgggctac acagcagaag aaatgcctga tgatatcact tttaagcgat agtacggcca
     1021 gagagtagtt gctgtgttcg tcgagcatgg taatgaaata acggtacagc tcgccattct
     1081 gagctcaata gtatccatgc cactgagctt acaggtcttt atcgggcgat agtgctttat
     1141 tcttcggggc tttgtggatg atttttcgaa atgttcatga tagttaggta aagctaccgc
     1201 atatcatttg caaaatgaca gatagatata cttcaatttg aatcttaaca attagtatgg
     1261 gaatgctgtc aatcctctgt atgcgtccac cacatattga aatttttgtt ctctaatata
     1321 ttccacgggc gtcaagagtg tgaggcctgt gctgttatac agcaccagac gttgtccccc
     1381 atctttgacc tgtctcttga tcagatctcc tgatcaagag acttcatcac caggtaaccc
     1441 tcagccatat cctgatgtct gaaccagcca tcccacataa ctacccaacc ggggctgctt
     1501 gtgcgtttgc tgtcatgcca tcgccccagt ttagccagtt tcaaacaggc ccatttcagg
     1561 gtcggtgttt gcgccggaag ctgctttcct tccagcttaa cccacagtag tttccactct
     1621 gtcggcgtca gtattttctc acagctgtca ttttgtgttt cttcactgat acctccctgc
     1681 cgcaggccca gcacccgcac cgcgataacc accatgcgct caaggttatc ccgggtctgc
     1741 attcgcagcg attccacaca tgtaccacca cttttccacg ctttgtggta ttcctctatc
     1801 agccagcgtt gctcgtaatg gctgacgata cgtcgcgcat cggcggcact cgccactttt
     1861 tctgatgtca gcggatacca gcaggctccg tcctctcctg ctccccgcaa cagacatacg
     1921 tgagcgagag cgcctggccg ctgttgtcgg gattttttat gctgacttcg ctgtaactga
     1981 tgagcatccg ggcctggcgg gctgcccgcc cgcctttttg catcacattc agcgtgtggc
     2041 ttcccgcggc tgactttata atgcaggtaa tgccagatat ccgcttcccg gtcacagaca
     2101 gtgattacct gtttctgtat ctccctcagc cgttcggcca tgcgctccga agcctgctgc
     2161 cagcggtaac tttctttttc ttcacaggga cgttcttttc gctggtgctt aacaccgtag
     2221 gtgtccgtgg cacgactcca gcactgctgt tcgataagat cgactggcag ggcgctgtcg
     2281 ggggcgtaca tcaggacaga gtgagccagc agcccgtgcg tcttcgggtt agtggtggta
     2341 ttccccaggt catcaaatgc cgtactgtga ccgaagttaa tggtggtggt atcttccagc
     2401 gcgaggagca gcggatgtgc ctcacatgcc cttgcagtgg cagtaaatcc ggcttcggca
     2461 atggcttacg gggacaccga cgggttacgt atcaggcggt acgcaccttc aacctgagca
     2521 gtgaagtggg atgatttcac aatagaaaga cctgcatgct gagcgagaga agaggtcagt
     2581 gacacaaggc gtcgtgtacg acgcggatca ccgagacggg catgtccaga ctgctcgtta
     2641 gccagtgaat aacaatcaga aagtaccata acagacccga agaaaatgaa atataagaga
     2701 agatcaacgg gtgaagaaaa agttcaaaaa atcgctgccg gggaggaagg aaactaccgg
     2761 atggaaagag tccccctaaa gcagactgac agacatcaca aatccccggg tggggatttg
     2821 tgtataagag acaggttaca ggtgtggtga ataagactgc aaatgtggca gctcatgccg
     2881 tggtgaatgc tgcactggcg gtggtacagg gaaataacgc ccgtgccagt gccgcaggtg
     2941 ctgccactgg agacgtggtg gggatgatag ccacagagat gtgcggaata tcccctggca
     3001 ctgttacatg taaaagataa ataacgaagc tacatccttc tctttatgaa cggctttata
     3061 ttagttttgc tgacaagatt gagctaaatc cggccaatga agaagagcag gttattcttt
     3121 aaaggtttga taacatgtaa cacattttga atattcagtc agtaagcctt aaaaatttac
     3181 ctggttatgg gctgactgat ataacaaagt aaatattcga tggggaatgc cggatgcaat
     3241 gaacaatgca ttaacgtatc gaaataatga tgcaggagca aactcagtct ggtgagtata
     3301 cattatgcca ttgatgcaga gtttaaagga acagggctgg ttcgttatgg aacaacattt
     3361 gtgcctgagg aaaggggggg gagtatctga ggcaacagaa gtttgtgggg agttcggttt
     3421 attaccatgc ttggtttttt gtcagtgtgt tcaaatc
//
LOCUS       DAZFAB010000220         3409 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZFAB010000220 DAZFAB010000000
VERSION     DAZFAB010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.221"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(265..1749)
                     /locus_tag="ACHJWA_004791"
     CDS             complement(265..1749)
                     /locus_tag="ACHJWA_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO4311211.1"
                     /translation="MRKIITHFKVVLTLLLPVTVSAQQIQWQSCMASQFNHWFGEEKP
                     SPDLLCGYLSVPLKYTDTGGDASYEKKSQVKLALTKLPAKSKHKGSILIISGGPGLPG
                     INPYINFDWPVTNLRESWDIIGFDPRGVGQSTPTINCRQSDTETQENITEKQQVLNKI
                     NACIHNTGAEVIRHIGSNEAVYDIDRIRQALGDKQLTAVAYSYGTQIAALYAERFPYN
                     VRSIVLDGVVDIDDLEDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSDRDKAITQ
                     FHELLSKLHHKPLLDSKGENISSDELISLTTDLLLWRSSWPTLATAIRQFSQGIVSNE
                     IETALSAPIASEESSDASGVILCVDQGDEQLTPEERKFRKEALANAFPAINFDNGRSD
                     SPDFCELWPIHSDLNKTRLKNTVLPSGLLFVAHKYDPTTPWINARKMAEKFSSPLLTI
                     NGDGHTLALTGVNLCVDKAVVHHLITPQKIENIYCPGNSEAEIQ"
     gene            complement(1936..2889)
                     /gene="ompT"
                     /locus_tag="ACHJWA_004792"
     CDS             complement(1936..2889)
                     /gene="ompT"
                     /locus_tag="ACHJWA_004792"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HFO4311212.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETLSFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVSVNAGYYVTPNAKVYVEGTWNRV
                     TNKKGNTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
BASE COUNT          935 a          640 c          708 g         1126 t
ORIGIN      
        1 gtagctctgg gtatgaagct gtttgtaact catgttacag taatggctat tactatctcg
       61 aaggtgcaaa atgtatattg atttgatatt ttataaattt acttcgaatc tggcacgtca
      121 acttttgatt aaagtaatat cagttttcgg cgaaaaaagg accgtgtaca tgagacgatt
      181 tcatacaaga ataacaaaaa tatgaatatg ctcccaatgc aatatatgtt ttgcattggg
      241 agcaaatagc ggaatatttt gaaattattg tatttctgct tcagaatttc ctgggcagta
      301 tatattttct attttttgtg gagtgatcag gtgatgtaca actgctttat ctacacataa
      361 attaactccg gtgagagcta atgtatgccc atcaccattt attgttagta acgggctgga
      421 aaatttctct gccatcttac gggcattaat ccagggcgtt gttgggtcgt atttgtgtgc
      481 tacaaacagt aaaccagagg gcagaacagt atttttcagg cgagttttgt tcaggtcgct
      541 atgtattggc cataattcac aaaaatcagg tgaatcggaa cgtccattgt caaagttaat
      601 agccgggaag gcattcgcaa gagcctcttt tcggaatttt cgctcttctg gtgttaattg
      661 ctcatccccc tgatctacac aaaggattac ccccgaagca tcacttgact cttctgaggc
      721 tatcggagca ctgagcgcag tttcaatttc attactgaca atcccctgag agaactggcg
      781 tatggcagtt gcaagggttg gccatgatga acgccatagc agaaggtctg ttgttaatga
      841 tatgagttca tctgaagata tattttctcc cttactgtct aataaaggtt tgtgatgtaa
      901 ttttgataat agctcatgga actgagttat tgccttatct ctgtctgaag aaagcgggca
      961 actttttgta cgcgcacacc aggatgcaaa gcgatcaaac gtttcctgat aactctgtgc
     1021 ctgtttgagt tgccatgtga agttgtcctc caggtcatcg atatcgacga ctccatcaag
     1081 aacgatagat cttacgttgt agggaaaacg ttctgcatat aaggctgcaa tttgagttcc
     1141 atacgaatac gccacggctg tcagttgttt atcccccaag gcttgcctaa tacgatcaat
     1201 atcgtataca gcctcgttag agccgatatg gcgaatgact tcggctccgg tattatggat
     1261 acaggcatta attttattta atacttgttg cttttcggtt atgttttcct gagtctctgt
     1321 atctgattgc cggcagttta ttgtcggagt ggactgcccg acgcctcgag gatcaaatcc
     1381 aataatatcc catgactcac gaagatttgt gactggccag tcaaagttaa tataaggatt
     1441 tatgcctggt aacccgggac caccacttat tatcaggata cttcctttat gcttgctttt
     1501 tgccggcaat tttgtcaacg ctagtttgac ttgtgatttt ttttcataag aagcatctcc
     1561 gcctgtgtct gtatatttta atggaacaga caaataacca catagtaagt caggagacgg
     1621 tttttcctca ccaaaccagt ggttgaattg actggccata caggattgcc actgtatctg
     1681 ctgggcagat acggttactg gtagaagtaa cgttaaaaca actttgaaat gagtaattat
     1741 ttttcgcatt gtgtctctga atatcggaat aaagataaaa tttgaatata ttgaggtctt
     1801 gtgttgcggt aagagattac acgttatgac ataggttaaa tgcttacaaa attagtggat
     1861 attgtctact tgtaactgta aacaaattcc ccggggttat acaataccac cggggagaaa
     1921 atctggttaa cttcgttaaa aggtgtactt aagaccagca gtagtgatga agttatagtt
     1981 ttctatgcct gcaccatttt tgctgtagtc tgaagtgtta tcattgtgat cataaagtga
     2041 agtattacct tttttattcg taacccgatt ccatgtgcct tcaacataaa cttttgcgtt
     2101 aggtgttacg taataacctg cattgactga aacagaatag taattttggt ctttgacttt
     2161 actgcgataa gtgattcttt ttcctgggtc atagtgctca tcgttatcag atgcttccac
     2221 ccagccgctg tatttaaatg tgccacctag ctcaaaatct tcataacgat aacttccagt
     2281 caagccaatg tagggcattt taaaacgttg tttgtagccg attgctcttt ctccattcgg
     2341 gaaggagccg atatcatctc tgaatccctc ctcagaactg tagatatagg aaccacctct
     2401 ggctgtaaag ctataacggc tttcctgata tccggccatg agtcccaggc ggtaattggg
     2461 ttcgttgagg agccagcctt tgatattcag atcaaattcg ttggcataat tgagttgtgt
     2521 atcagggtgt ctactttcat ccgtccaggt tccggggtta ctggaatcca tccagtcctg
     2581 atcgaccata ttgccacctc ggctgccgag agttgtccag ccagcagccc cgatagatat
     2641 ctggggcatc aaatcccaat taattgcacc tttaataatt gcagcgttat tgaatttcca
     2701 gtcgagttga ctgacttttc ggcctccttc ttcggctaga taaacacgct cttttgtttt
     2761 tccgctcaga gttccaagac taatgtccgc atttatgttg tcaggagtaa acgataaagt
     2821 ctcggtagaa gcaaaagagc tgatcgcaat aggggttgtc aggactattc ccagaagttt
     2881 cgcccgcata aaaggtctcc attcaatcgt tttaatgatt gaatatgtat tttttatatc
     2941 taacttaatg agtcaattgt atattgttta actgtttatg gtttgtttaa gtttgaatta
     3001 ttgtttcgtt ttattcattc tttatgggca gcttgccaag tgttgtatta ttgttgtggt
     3061 ttaatatgct ggaaattttc tttttgctga tatttttatc tgtctatagt tattcattta
     3121 tacgcatatt agcaaaaaaa atattatttt aggtatttct acagtacatt tgacacgatg
     3181 attaacctct atgtagctag gtttttgtaa agaaactgtg cgtcactgta tccaggactg
     3241 ttatgtgcca aataaggaag ttgaagttac cagctcttac tctaaagcta taacgtatag
     3301 aaaaaatgca tttgagacgt gtaaaatcat ttatttaatc gcaatttatg taatggcata
     3361 atcaggtcgg tgaaagaggg gagaagaatg gataaaagag ctaaaaatc
//
LOCUS       DAZFAB010000221         3381 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZFAB010000221 DAZFAB010000000
VERSION     DAZFAB010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.222"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(119..442)
                     /locus_tag="ACHJWA_004793"
     CDS             complement(119..442)
                     /locus_tag="ACHJWA_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFO4311213.1"
                     /translation="MKRTLLIAVWAIGLMSDSAMALTLNEARSQGRVGETLNGYLVAL
                     QTDAETQALVKDINEARNHSYQQLAKQNNVSTKEIAKLAGQKLVARAKSGQYVQGVNG
                     KWLRK"
     gene            complement(450..635)
                     /gene="ynbE"
                     /locus_tag="ACHJWA_004794"
     CDS             complement(450..635)
                     /gene="ynbE"
                     /locus_tag="ACHJWA_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFO4311214.1"
                     /translation="MKIVIAAFASSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEDLLKTRSDLF"
     gene            complement(632..3271)
                     /gene="ydbH"
                     /locus_tag="ACHJWA_004795"
     CDS             complement(632..3271)
                     /gene="ydbH"
                     /locus_tag="ACHJWA_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFO4311215.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPSVKLVGEFTMPLMPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANAGPMTLRLDKDTADAVAKDNMTAGTAINWLRYMEIS
                     RSSTRINLDNLGVLTMQANISGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQV
                     WLEQNAQLPETGCPSGKECEEKQ"
BASE COUNT          835 a          916 c          771 g          859 t
ORIGIN      
        1 agggaaggat atataaggtt tttgtagaaa tacccacggt aattaagcat gctaacgtca
       61 gtttaaatac tgtaaaagat cattacatta atagatacat tttgtagcca actataattt
      121 atttgcgcaa ccatttacca ttaactccct gaacatattg ccccgacttc gcgcgagcaa
      181 caagcttttg tccagccagt ttggctatct ctttagtgga tacattattt tgctttgcca
      241 gttgttggta actatggtta cgagcttcat taatatcttt tactaatgcc tgtgtttcgg
      301 catccgtttg tagcgccacc aggtaaccgt taagcgtttc acctacccgc ccctgacttc
      361 tggcttcgtt cagggttaaa gccattgcac tgtcgctcat caggccaatg gcccatacag
      421 ctatgagtaa tgttcttttc atcatcacct caaaatagat cgctacgggt tttgagcagg
      481 tcttcaacgt ccttatccgc tttaatgatg atttcatgct caattttaac gttcatatta
      541 atggtgattg gctctttcgg cgcagccact tcaatgcgag gcgtgcaacc aaccaacata
      601 aaagaggacg caaacgcggc tatcactatt ttcattgttt ttcctcacac tcttttcctg
      661 atgggcagcc agtttccggt agctgggcat tctgctccag ccatacctgg agattatcgc
      721 caaagcgtaa actgcgccat aacgtaaaaa tattctcttc atggtgataa ttaaggttta
      781 ccgtaccgct tttaccatcg acgcgactgg tgcccgaaat gttggcctgc atggttaaca
      841 caccgagatt atctaaatta atccttgttg atgagcgact gatttccata taacgcaacc
      901 agttgatggc agttccagcg gtcatattgt ctttggccac cgcatctgca gtgtctttat
      961 ccagacgcag cgtcatcggc cccgcgtttg ccaaccagcc atctttgaca atccattttt
     1021 cattgttcag ccatagtggc aacgccccac taaatgcccc agacatagca aactgtttcg
     1081 gattaaccgc gctaaccagt tcgctggatg agagattatt cagccgcaac agcgccgggt
     1141 catgttgcgg catacgtaat tgtttcatca gcacgttacc gcctaacaca tcgacgctaa
     1201 catcagtcaa cagcaatggt tcttcttcag tccacggata acgcccttgc aaatcagccg
     1261 taatattttt cgctgtcacc agattaatca cttcggcaat tcgcaacgta acggggccgc
     1321 gagtccccag atgccaggct ccatcggcaa aacggaaagg caggacaaaa tcgacaccgt
     1381 taacctgatt atctggcatc caggcactac cgcctttcaa cacgccgtgc cctcccgcgc
     1441 ggaatccttg ttcaggcgca gcagaaaatg caacctgagc atacagttca ccatcgcgta
     1501 agttcatctt ccagtcgggt ggcaccagcg gctggaatac agtcagtgat tgtttaggcc
     1561 accaggcgtt accgcgcaga cgaataccgt cccagcgacc atttacccga accgggccaa
     1621 tctcaccagc atgtaaatcg cctttaaaga ggaaatacgt cggatcgcgc ccatcgacgc
     1681 taaattttaa ggttgatggc ggtaacacac tgccgccagt gaacagcgtt tgcccggcgt
     1741 ccagtgacag cgcgccgctg aaggagggat gctgtgcgtc acgtacccag acgacgggct
     1801 tgtcgagaat taatcgcggc ttttctaccg tcatcgtacc gtattgtaac tggtcgaaac
     1861 cggtggagag atcggtcagc gtaatcgtgc tgtcatgcca ctcacctttt cctgcgacat
     1921 cccaggtggc attcatcggt gtaaaactcc cttttcccca gtagcgccac tgccagcggc
     1981 cagcgtcagg gagaaaatca ttcgccagcc catccatatg cagcacgaaa tcgcccagtt
     2041 cattttcatg cgcctgcaaa atggcctgca aacgtccgtc aacaccacgt tgggtgactt
     2101 ttacacccgc taaaggccag cggatttcat cgatgtccag cgaatcgatg actcttccct
     2161 ttgaacgaag taacgcgcct ggctcaaagg tcagcgttgg gtcagacaga cttccactta
     2221 gctgcgcagg taaacgggca tataaaatga ggtccgcctg tttcgcttca ccggtcagct
     2281 gcagaggcag ctggctgtta tccatgctta attttcctgg gccaaaatta agcaccgcgt
     2341 tgcccttacc cgcttgcccc tgggtcagca cactcagtcg tccgctgacc agagcgttct
     2401 caagccctgc ctgccagttg tcgactttga cacccagtcg gccactcaaa ggaaaacctg
     2461 catacggcca gctccagcga ccatcgctta cggtcaattg ttgacgagta atttgccacg
     2521 gcaaatcgag caacggatcg ccgttatccc gtgccagcac gatcaattgc ccgctatttt
     2581 cctgccagtc cagctcggca tccaccagtg acggttcctg cggcaagttt aacgtcgcag
     2641 tagcatgccc acttacagga agtccatccg gcatgagcgg catagtaaat tcccccacca
     2701 gtttaaccga cggctgattt tcaaacgcga cgacatccag ttcgctgact gtaagttgtt
     2761 gccctttcag ctggccttga aatttaactt tttcgccctg ataacgcagt tgctggatat
     2821 cagaggttaa tgcgagagag agttttccct gccattcctg ccagggagaa aaaatcagtt
     2881 tatcaatatt gatccaggtg ttaggcagca tggactgcca ttgcgcgagg gtttttggag
     2941 cagctggcga ttgctccgtc tgcggcaatt tcgccagaca agcagaatca agttctaccg
     3001 tgccgacgtt caataaccag cggctgggat gtgaaaggct ggcgttggtg atatgcgcaa
     3061 gctgacaatc tcccaccaga taacggagat cgggaatgat taaaccttta cgcgtaatgc
     3121 gtgggctttc atctaatgca atacgtgtcc cgagcggtag ccagatgccc gccagcgtgg
     3181 gaacccacag cccgagcgtc atcagcagcg tcaacggcac aagaataatc agtaataaca
     3241 gcgcgagaac ggctttatat ttacccagca tgggtagtta atatcctgat ttagcgaaaa
     3301 attaagcatt caatacgggt attgtggcat gtttaaccgt tcagttgaag gttgcgccta
     3361 cactaagcat agttgttgat g
//
LOCUS       DAZFAB010000222         3362 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZFAB010000222 DAZFAB010000000
VERSION     DAZFAB010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.223"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            31..390
                     /locus_tag="ACHJWA_004796"
     CDS             31..390
                     /locus_tag="ACHJWA_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO4311216.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVVLIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYKIESE"
     gene            399..929
                     /locus_tag="ACHJWA_004797"
     CDS             399..929
                     /locus_tag="ACHJWA_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFO4311217.1"
                     /translation="MIYPEITGKSGEHLRLNTLEAVWIQGKLRMWGRWSYIGGGKSGN
                     MFNRLLVSKKLTKTAVNEVLRSMKKSGLEKPELEAFFRDMTRGKQKSWLSHCTDTEAL
                     IIDRVISEVLGEYPGLINILRQRYEGRGMSKRKMAECLNRTHPEWCFSTCEKRIAGWL
                     AVAEHMLYVPMHDSFR"
     gene            1171..1368
                     /locus_tag="ACHJWA_004798"
     CDS             1171..1368
                     /locus_tag="ACHJWA_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO4311218.1"
                     /translation="MLKQQDMTETARVVFNELSVTDPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            1519..2577
                     /locus_tag="ACHJWA_004799"
     CDS             1519..2577
                     /locus_tag="ACHJWA_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO4311219.1"
                     /translation="MLNTVKISSCELINADCLEFMRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWAGDEDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDTEIMMRERFNVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYEAKGRTLKQHVMA
                     PLIAYFRDARAVLGITAKQIADATGKKNMVSHWFSAGQWQLPNESDYLKLQALFARVA
                     EEKHQRGELEKPHHQLVDTYASLNRQYAELQSEYKHLRRYFGVTVQVPYTDVWTYKPV
                     QYYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAAMALGRRATGVELET
                     ERFEQTVREVQDLIIRNG"
     gene            2618..2693
                     /locus_tag="ACHJWA_004800"
     tRNA            2618..2693
                     /locus_tag="ACHJWA_004800"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2651..2653,aa:Ile,seq:cat)
     gene            2774..2850
                     /locus_tag="ACHJWA_004801"
     tRNA            2774..2850
                     /locus_tag="ACHJWA_004801"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2808..2810,aa:Arg,seq:tct)
BASE COUNT          861 a          731 c          954 g          816 t
ORIGIN      
        1 actggcgtgc tcgggtaaaa gaggtgactg atgctcatag atttggtttt accttacccg
       61 ccgacggtga acacttactg gcgacgccgt ggcagcacat attttgtatc aaaagccggg
      121 gagcgttatc gccgggcagt ggtgcttatt gttcgccagc agcgactgaa attaagcctg
      181 tccggacggc tggcaataaa aattattgca gagccaccgg ataagcgtcg tcgtgacctg
      241 gacaatattc tgaaagcacc gctggatgcg ctgacgcatg cgggagtgtt aatggacgat
      301 gagcagtttg atgaaatcaa tattgtacgt ggtcagccag tatctggtgg acggctgggt
      361 gtgaagattt acaaaattga gagtgagtga gcgtaaatat gatatatccg gaaattacag
      421 gcaaaagcgg cgaacattta cgcctgaaca cgctggaagc agtctggatc caggggaaat
      481 tacggatgtg ggggcggtgg tcgtatatcg gtgggggtaa atccggaaat atgtttaacc
      541 ggttactggt ttcgaaaaag ctgacgaaaa cagcagttaa tgaggtttta cgcagcatga
      601 agaaatccgg gctggaaaaa ccggaacttg aggcattttt tcgggatatg accagaggga
      661 agcagaagag ctggttgtca cattgtacag acacagaggc gttgattatt gatcgcgtta
      721 tcagtgaggt gcttggggaa tatcccgggc taatcaatat tctccggcaa aggtacgaag
      781 gacggggaat gagtaagaga aaaatggcag aatgtttaaa tcgtactcac ccggaatggt
      841 gtttcagcac atgtgagaaa cgtattgcag gttggttagc cgtggctgaa cacatgcttt
      901 atgtacctat gcacgattca tttcgataaa aaaagcttgc ttttttacgc agaaacagct
      961 tgaattcctg taagcttcgc aaagctgtat cgcgaggcga aatgcaagtt ttttcgcaca
     1021 aggaagccac cggaaggtgg tttttttgtg tccgtaatat acagcagcgc aataaattcg
     1081 ctggtggtta ttaataccgt tctttcaggt tgctggcttt ttcgacaaga gttattggtg
     1141 tgtcacgtta accggaaaag ggaaaaagac atgctgaaac agcaggatat gacagaaacc
     1201 gccagagtgg tgtttaatga attaagcgtt accgacccgg cgacagtcgg ggagattgcg
     1261 cagaatactt acctttcacg cgaacgctgc cagttaatac tgacccagct ggttatggcg
     1321 ggtctggcag actatcagtt tggttgttac agacgccttc agtcctgaag gcttttttat
     1381 ttgtggtaaa tgggcggctg gtgggtgtaa ggggcaccca ccagccatct gctcatgcgt
     1441 tgggttcaca agcaaacctc aggcccactg ctttgcgcaa aagcagaatg agcctatcag
     1501 agacaggctt aatgatccat gcttaatact gtaaaaatat ccagttgtga gttaatcaac
     1561 gccgactgcc tggaatttat gcggtcgtta cccgaaaatt ctgttgacct gatagtcacg
     1621 gacccgccgt acttcaaagt gaaacccgag ggctgggata accagtgggc gggtgatgaa
     1681 gattacctga agtggctgga ccagtgtctt gcgcagttct ggcgggtgct gaaacctgcc
     1741 ggaagtcttt acctgttctg tggccatcgt ctggcatctg acaccgaaat catgatgcgt
     1801 gagcggttta acgtgctgaa ccatatcatc tgggcaaagc cgtccggacg ctggaacggg
     1861 tgcaacaagg aaagcctgcg ggcgtatttc cccgccacag agcgcattct gttcgcagag
     1921 cattatcagg ggccgtatcg tccgaaagat gccgggtatg aggcgaaggg taggacactg
     1981 aaacagcatg tgatggcccc gctgattgct tactttcgtg atgcgcgcgc tgtcctgggg
     2041 ataacggcaa aacagattgc agatgccaca ggaaagaaaa acatggtgtc gcactggttc
     2101 agtgccggtc agtggcagct gccgaacgaa agcgattatc tgaaattaca ggcactgttt
     2161 gcccgggtgg cagaagagaa gcatcagcgg ggtgaactgg aaaagcccca ccaccagctg
     2221 gtggatacgt atgcctctct gaaccgacag tatgcggagc tgcagagtga atataagcat
     2281 ctgcggcggt atttcggtgt gacggtgcag gtgccgtaca ccgatgtgtg gacgtataaa
     2341 ccggtgcagt actatccagg gaaacatccg tgcgaaaaac cggcagaaat gttgcagcag
     2401 ataatcagcg caagcagtcg tccgggagac ctggttgcag atttcttcat ggggtcgggg
     2461 tcgacagtga aagcagcgat ggcgctggga cgtcgtgcaa ctggcgttga actggagact
     2521 gaacgttttg agcagacggt gcgggaagta caggatttaa tcattcgtaa cggatgagat
     2581 tgcggagtta atcatgcgtc gttattattc agcaatcggc cctttagctc agcggtgaga
     2641 gcgagcgact cataatcgcc aggtcgctgg ttcaaatcca gcaagggcca ccaaccgcca
     2701 ctagctcatc aggaaagaac gtcaccctgt gcgagattcg gagtccccgg tggcggtcca
     2761 ttatcggtat tctgcgttgt tagctcagcc ggacagagca attgccttct aagcaatcgg
     2821 tcactggttc gaatccagta caacgcacca caccacactt atctgccctg actctctttt
     2881 gcgggctttt tattacagga aagacaccgg acagtgaaat gttaaatgcc tcacaattca
     2941 ggcagttgac tgttgcctga catgctgagc gtttgttaaa aaaatcctgc atgatgaatc
     3001 cccctgagcg gcggggcata atgacagatg tttggttgcg tattgtatag gcaagttgcg
     3061 gattctgtct ggtcattgca gaattcaccg ggaggcaccc ggcatcatgc tgtatacaga
     3121 gattaggcat atatccaggc ttctcatcgc aggagccttt ttacatgcaa aaaaaagccc
     3181 gagtgggttc gggcaacagc atgagatact tgcattgtca tttttatcgt gtggatttta
     3241 accagggttt ataaggctgc gcaactgcgc ggcctttttc gttttgcggg ctgcggttct
     3301 cctcttttga ttctccttgt ggccggaccg tggcccgcaa ctgttgagga aaatcccgga
     3361 aa
//
LOCUS       DAZFAB010000223         3330 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZFAB010000223 DAZFAB010000000
VERSION     DAZFAB010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.224"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            283..1965
                     /gene="ydeN"
                     /locus_tag="ACHJWA_004802"
     CDS             283..1965
                     /gene="ydeN"
                     /locus_tag="ACHJWA_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO4311220.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQVPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPEQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            2017..3174
                     /gene="ydeM"
                     /locus_tag="ACHJWA_004803"
     CDS             2017..3174
                     /gene="ydeM"
                     /locus_tag="ACHJWA_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO4311221.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKLGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNDETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(3140..3262)
                     /locus_tag="ACHJWA_004804"
     CDS             complement(3140..3262)
                     /locus_tag="ACHJWA_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311222.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
BASE COUNT         1007 a          704 c          666 g          953 t
ORIGIN      
        1 acatatttat gcatttgcat aacttgttgc atgattattt atgatctcaa ttcggtattt
       61 tgttagtaaa atacaataat ttattaaata tcaatagatt aattgtttat cgtcgagaaa
      121 ttacttagta gaacaaaaag taatgtcaac gctttatgga ctgttatttc ccttttttta
      181 gctaaatctg ctatctcttt atgtgactaa cttcacttac atccacttat ttctcttcgt
      241 aaaattactt tggaattaag tacaataaga agaggaacat ttatgaagtc tgcattaaag
      301 aaaagtgtcg taagtacctc gatatctttg atactggcat ctggtatggc tgcatttgct
      361 gctcatgcgg cagatgatgt aaagctgaaa gcaaccaaaa caaacgttgc tttctcagac
      421 tttacgccga ctgaatacag taccaaagga aagccaaata ttatcgtact gaccatggat
      481 gatcttggtt atggacaagt tccttttgat aagggatctt ttgacccaaa aacaatggaa
      541 aatcgtgaag ttgtcgatac ctacaaaata gggatagata aagccattga agctgcgcaa
      601 aaatcaacgc cgacgctcct ttcattaatg gatgaaggcg tacgttttac taacggctat
      661 gtggcgcacg gtgtttccgg cccctcccgc gccgcaataa tgaccggtcg agcgcccgcc
      721 cgctttggtg tctattccaa tactgatgct caggatggta ttccgctaac agaaactttc
      781 ttgcctgaat tattccagaa tcatggttat tacactgcag cagtaggtaa atggcacttg
      841 tcaaaaatca gtaatgtgcc ggtaccggaa gataaacaaa cacgtgacta tcatgacaac
      901 ttcaccacat tttctgcgga agaatggcaa ccgcaaaacc gtggctttga ttactttatg
      961 ggattccacg ctgcaggaac ggcatattac aactcccctt cactgttcaa gaatcgtgaa
     1021 cgtgtccccg caaaaggtta tatcagcgat cagttaaccg atgaggcaat tggcgttgtt
     1081 gatcgtgcca aaacacttga ccagcctttt atgctttacc tggcttataa tgctccgcac
     1141 ctgccaaatg ataatcctgc accggagcaa tatcagaagc aatttaatac cggtagtcaa
     1201 acagcagata actactacgc ttccgtttat tctgttgatc agggtgtaaa acgcattctc
     1261 gaacaactga agaaaaacgg acagtatgac aatacaatta ttctctttac ctccgataat
     1321 ggtgcggtta tcgatggtcc tctgccgctg aacggggcgc aaaaaggcta taagagtcag
     1381 acctatcctg gcggtactca caccccaatg tttatgtggt ggaaaggaaa acttcaaccc
     1441 ggtaattatg acaagctgat ttccgcaatg gatttctacc cgacagctct tgatgcagcc
     1501 gatatcagca ttccaaaaga ccttaagctg gatggcgttt ccttgctgcc ctggttgcaa
     1561 gataagaaac aaggcgagcc acataaaaat ctgacctgga taacctctta ttctcactgg
     1621 tttgatgagg aaaatattcc attctgggat aactaccaca aatttgtccg tcatcagtca
     1681 gacgattacc cgcataaccc caacactgag gacttaagcc aattctctta tacggtgaga
     1741 aataacgatt attcgcttgt ctatacagta gaaaacaatc agttaggtct ctacaaactg
     1801 acggatctac agcaaaaaga taaccttgcc gccgccaatc cgcaggtcgt taaagagatg
     1861 caaggcgtgg taagagagtt tatcgacagc agccagccac cgcttagcga ggtaaatcag
     1921 gagaagttta acaatatcaa gaaagcacta agcgaagcga aataactaaa ccttcatgcg
     1981 gcggattttt ccgccgcctt attgagcgag atagcgatgc acgttacagc caagccctcc
     2041 agttttcaat gtaatctcaa atgtgattac tgtttttacc ttgaaaaaga gtcgcagttt
     2101 actcatgaaa aatggatgga tgacagcact ctgaaagagt tcatcaaaca atatatcgca
     2161 gcgtctggca atcaggtcta ttttacctgg caaggcggtg aacccactct ggctggcctg
     2221 gattttttcc gtaaagttat tcactatcaa caacgctatg caggccaaaa acgtattttt
     2281 aatgcattac aaacgaatgg cattttattg aataatgaat ggtgtgcctt tctcaaagaa
     2341 catgagtttc tggttggtat ctcgatcgat ggaccccagg agctacatga ccgttacaga
     2401 cgcagtaatt caggtaacgg tacttttgca aaagtgatag cagccatcga gcgtctgaaa
     2461 tcatatcaag tagagtttaa tacgttaacc gtcattaata acgttaatgt ccattaccct
     2521 cttgaggttt atcatttttt aaaatctatc ggcagtaaac atatgcaatt tatcgaattg
     2581 ctcgaaaccg ggacgccgaa tattgatttc agtggtcata gtgagaacac attccgtatc
     2641 attgattttt ctgtgcctcc cacggcttat ggcaagttta tgtcaaccat ttttatgcaa
     2701 tgggttaaaa acgatgtggg tgaaattttc atccgtcagt ttgaaagctt tgtcagccgt
     2761 tttttgggga atgggcatac cagttgtatt ttccaggagt cctgcaagga taatctggtt
     2821 gttgaaagta atggagatat ttacgaatgc gatcattttg tctatccaca gtacaaactt
     2881 ggaaacatta ataaatctga acttaaaacg atgaacagtg tacaactgac agcgcaaaaa
     2941 aaacggattt cagcgaaatg tcagcaatgt gcatataaac ctatctgcaa tggcggttgc
     3001 cctaagcatc gtattactaa agtaaacgat gagactgttt cctatttttg cgaaggttat
     3061 aaaatccttt tttcaaccat ggtaccttat atgaacgcca tggtggagtt agccaagaac
     3121 agagtaccgc tttaccacat tatggatgtt gcaaaacaaa tggagaataa ttaataacgc
     3181 tctcttttag ctccattctt agcctccttc ttatgacctt aacaaggcac ccgacataaa
     3241 ttcctgtcgg gtgtttcgtc atcatcatct tcccttgcga aaatcttggc ttacgtggta
     3301 tgcgtcaggg cattagcaat acccttgatt
//
LOCUS       DAZFAB010000224         3326 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZFAB010000224 DAZFAB010000000
VERSION     DAZFAB010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.225"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..384
                     /locus_tag="ACHJWA_004805"
     CDS             <1..384
                     /locus_tag="ACHJWA_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO4311223.1"
                     /translation="LAEFVALISESGANPFGLTVDAVMEEYRRWRNESWRYDGSDKYP
                     WPQPVLYHICLEMRDRGIERQMTEGELKRLAERQLTKWAKQVGNGMSIPPIRRQLASP
                     KCPQGPTPIELLKQEYERRKAAGFV"
     gene            418..1134
                     /locus_tag="ACHJWA_004806"
     CDS             418..1134
                     /locus_tag="ACHJWA_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO4311224.1"
                     /translation="METVSDALKALKRASSHVVAARLGISREEAVNELWKLKRRGEAD
                     NKGAIWWLTQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGCLIRVNQDGKFRYCIPGVDLPAEPKAASVAETEGKALPQPAGVALPVQETA
                     AQEEIKTEAVEDIVKLQQSFTEAKADDLILPSLHVANRELRRAKSNVQKWERVCAALR
                     ELNKHRDILRDITATREQQR"
     gene            1131..1448
                     /locus_tag="ACHJWA_004807"
     CDS             1131..1448
                     /locus_tag="ACHJWA_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO4311225.1"
                     /translation="MSGWKKWRWAEILILRQCAGTMRVESIGYLIGRSESAVRTKARE
                     LGISMMLRGDYHQSAKCSQRDIELAWQLHQRGVPRREIAEKFGMKLGAVNNYVYFDRR
                     VQE"
     gene            1445..1747
                     /locus_tag="ACHJWA_004808"
     CDS             1445..1747
                     /locus_tag="ACHJWA_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFO4311226.1"
                     /translation="MRVRVYIAGPMTGYENFNREAFHKTEEVLKREGHTVLNPAVLPD
                     GLTQPHYMDICMAMLRCVDAVYMLKGWQQSAGAGAELALEEKPGHAVIFQEVGSEY"
     gene            1737..2054
                     /locus_tag="ACHJWA_004809"
     CDS             1737..2054
                     /locus_tag="ACHJWA_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4752 family protein"
                     /protein_id="HFO4311227.1"
                     /translation="MNIDPAITIDMALNAGLALLGYFYIMFCSGRWLSLLFMKKWNKR
                     RKQEQRQKAMDAFFEAFGIDGMEPGDPARAISRGGVVILVYRSEEKNDDHKTTCRRNH
                     IPH"
     gene            2008..2325
                     /locus_tag="ACHJWA_004810"
     CDS             2008..2325
                     /locus_tag="ACHJWA_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311228.1"
                     /translation="MTITKQRVEEIISRIEMYGHGAGYIADEVNDLAILALNLSNIAN
                     LKRYELDMGGCDSCGQDCGADMTEDPDGDYVLFDDVVKLFEFDTTTQKLEIPAKEAAS
                     GQD"
     gene            2312..2749
                     /locus_tag="ACHJWA_004811"
     CDS             2312..2749
                     /locus_tag="ACHJWA_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO4311229.1"
                     /translation="MGKIDYQALREAAQNYQSTLAWYQATPDSPNAERDCDAALAAFK
                     RHIRHREADIIADLLDGLEEAKSQLNEQREYYEGVISDGSKRIAELEAREVQLPTRYD
                     LRYGHPINADERHVMIPKENGSWLYLIDLEHALRVAGIRIKGE"
     gene            2751..2942
                     /locus_tag="ACHJWA_004812"
     CDS             2751..2942
                     /locus_tag="ACHJWA_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311230.1"
                     /translation="MNGQISIVRPGACDDREIRMIIRLAMGKTITALITPENLALALT
                     GKSDMPVELKLRNVEIKVK"
     gene            2945..>3326
                     /locus_tag="ACHJWA_004813"
     CDS             2945..>3326
                     /locus_tag="ACHJWA_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311231.1"
                     /translation="MTTITKDRLLTIQHWRETYGPGSNVVLPAEEAEELARIALASLA
                     AVSDERAAYELFMEKRFGESVDRRRAKNGDRDYMVWDMALGWIIWCHRAAMLQAGNFR
                     ENKGSSTNNFREISETSTNYPVTPD"
BASE COUNT          920 a          693 c          992 g          721 t
ORIGIN      
        1 ctggctgagt ttgtggcgtt gatttcggag agcggggcaa atccatttgg cctgacggtg
       61 gatgccgtga tggaggagta ccgtcgctgg aggaatgaat cctggcggta cgacgggagt
      121 gataaatacc cgtggccaca gccggtgctg taccacattt gcctcgagat gcgtgacaga
      181 gggattgagc ggcagatgac ggaaggtgag ttaaaacgac ttgcagaacg gcaactgacg
      241 aaatgggcaa agcaagttgg taacgggatg agtattcccc cgatccggag acaactcgca
      301 tcgccgaagt gcccgcaagg gccaacgcca attgaattac tgaaacagga atacgagcgc
      361 cggaaggcgg ctggttttgt ttgaatctga gaaacgattt tgtcggagga aattttaatg
      421 gaaaccgtat ctgacgcact gaaagcactg aaaagagcct cttcacatgt ggtggcagct
      481 cgccttggaa ttagtcgtga agaggctgtc aacgaactgt ggaaactgaa acgccgtgga
      541 gaagcggata acaagggggc gatatggtgg ctgactcagg ctggtgaaag tgaaccggtg
      601 tcaccggtac cgaaagtgac ggcgcaaatg ctgacagagg cgattgaaca acatggccca
      661 caaacggcgg atgaactggc attgatgttc ggaattacct cccgccgggc gaattcatcg
      721 ctggcaatgg caatcagcaa agggtgtctg attcgcgtga atcaggatgg taaatttcgt
      781 tactgcatac cgggcgttga tttaccggca gagccgaaag cagcatccgt agctgaaacg
      841 gagggtaaag cccttcctca gccagctggt gttgcgttac cagtccagga aacggctgca
      901 caggaagaaa ttaaaacaga agcggtggag gacattgtga agttgcagca atcgttcact
      961 gaagcgaaag cagatgacct gattctacca tcgctgcatg tggctaaccg cgagctgcgc
     1021 cgggcgaaaa gtaatgttca gaagtgggag cgagtctgtg ctgcgctacg ggaactgaac
     1081 aaacacaggg atattctccg ggatattacc gccaccagag agcagcagcg gtgagtggct
     1141 ggaagaagtg gcgctgggct gaaatcctga tactccggca gtgtgcggga acgatgagag
     1201 tcgaaagcat cggttatctg attggccgta gtgagtcagc cgtcaggacg aaagcgcggg
     1261 aactgggtat cagcatgatg ttacggggtg attatcacca gtcagccaaa tgttcacagc
     1321 gtgatattga gctggcgtgg caactgcatc agcgtggtgt accccgacgg gaaattgccg
     1381 aaaagtttgg gatgaagttg ggcgcagtga ataactacgt ttatttcgac aggagggttc
     1441 aggagtgagg gtgagggttt atatcgccgg tccgatgacg ggatatgaaa attttaaccg
     1501 cgaggcattt cacaaaacgg aagaggtgct gaaacgggaa gggcataccg ttttaaaccc
     1561 ggcagtactt ccggacgggc tgactcagcc acactacatg gatatttgca tggcaatgct
     1621 ccgttgcgtg gatgcggttt acatgctgaa aggctggcag cagtcggcag gtgcaggggc
     1681 tgagctggca ctggaggaga aaccgggcca tgcggtgatt tttcaggagg tgggcagtga
     1741 atattgaccc ggcgataacg attgatatgg ccctgaacgc cggcctggca cttcttggtt
     1801 atttctacat tatgttctgc agcggacgat ggctgtcact gttgttcatg aaaaaatgga
     1861 ataaacgccg taagcaggag caacgccaga aggcaatgga tgcatttttc gaagccttcg
     1921 gaattgacgg catggaacca ggggatccag ctcgcgcaat cagcagaggg ggtgtagtaa
     1981 tccttgtata tcggagtgaa gagaaaaatg acgatcacaa aacaacgtgt agaagaaatc
     2041 atatcccgca ttgaaatgta tggacatggt gcagggtata tcgcagacga ggttaatgat
     2101 ctggctatac tggcgctgaa tttatcaaat atcgcgaacc tgaagcgata cgagcttgat
     2161 atgggaggtt gcgactcgtg cggtcaggat tgtggcgctg atatgactga agatcctgat
     2221 ggtgattatg tcctgtttga tgacgtggtt aagttgtttg aatttgatac aaccactcaa
     2281 aagttagaaa tcccggcaaa ggaggcagcc agtgggcaag attgactatc aggcactgcg
     2341 tgaggcagca caaaactatc aatcgacgct ggcgtggtat caggctaccc cggacagccc
     2401 aaatgctgaa cgggattgtg atgcggctct tgctgcgttt aagcgtcaca tccgtcatcg
     2461 ggaagcggat attatcgctg atttgctgga tggactggaa gaagcaaaat cacaactcaa
     2521 cgagcagcgt gagtattacg aaggcgttat ctctgatggg agcaagcgta ttgctgaact
     2581 ggaagcgcgg gaagttcaat taccgactcg ctacgacctt cgatatggac acccgataaa
     2641 tgcagatgag cgacatgtca tgatacctaa agaaaatggc agttggcttt acctgattga
     2701 cctagaacac gcattacgcg tcgctggcat tcgcatcaaa ggagagtgag atgaacggac
     2761 aaatatcaat tgttcgacca ggagcatgtg acgatcgcga aatacgaatg attattcgtc
     2821 tggcgatggg gaaaacaata actgctctca ttactccaga aaatctcgca ttagcattaa
     2881 caggaaagtc agacatgcca gtagagctaa agctgcgaaa tgttgagatt aaggtgaaat
     2941 agctatgacc actattacca aagatcgact gctgacaatc cagcattggc gcgaaacata
     3001 cggaccgggt agcaacgttg tgcttccagc agaagaagcg gaagagctgg cacggattgc
     3061 gctggcatca ctggcagcag tatcggatga acgagcagcc tatgaattat ttatggagaa
     3121 gcgtttcgga gaatctgtag atcgccgcag agcaaaaaat ggcgatagag attacatggt
     3181 atgggatatg gcgcttggct ggattatctg gtgtcaccgc gccgccatgc ttcaggctgg
     3241 aaactttcgg gaaaataagg gttcgtcaac caataatttt cgggaaatct cggaaacgtc
     3301 aaccaactat ccggtaactc cggatg
//
LOCUS       DAZFAB010000225         3294 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZFAB010000225 DAZFAB010000000
VERSION     DAZFAB010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.226"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..157)
                     /locus_tag="ACHJWA_004814"
     CDS             complement(<1..157)
                     /locus_tag="ACHJWA_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication protein P"
                     /protein_id="HFO4311232.1"
                     /translation="MKHIAAQMVNFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFP"
     gene            complement(154..1173)
                     /locus_tag="ACHJWA_004815"
     CDS             complement(154..1173)
                     /locus_tag="ACHJWA_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO4311233.1"
                     /translation="MTGSEMAKAGLLEQNRLSGANRNTLIAGGIMANTAEIFNFPVPD
                     AAQKEPRVADLDDGYTRIANELLEAVMLAGLTQHQLLVFLAVMRKTYGFNKKLDWVSN
                     EQLSELTGILPHKCSAAKSVLVKRGIFIQSGRNIGINNVVSEWSTLPESGKKNKVYLK
                     EVNLPESGKKSLPKSGKGTYPNQVNTKDKLTKDNIKPYSSENSGESSDLPENDLPVVK
                     ADAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFAGWANDIRLMRERDGRNHR
                     DMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQAGVTASKPKLDLTNTDWI
                     YGVDL"
     gene            complement(1170..1709)
                     /locus_tag="ACHJWA_004816"
     CDS             complement(1170..1709)
                     /locus_tag="ACHJWA_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT domain-containing protein"
                     /protein_id="HFO4311234.1"
                     /translation="MQPLTYQQTSGFSPTAVINRSQIKQVPGHEKIRDAVRAWSAVDN
                     QDVVATLIVNEYREQGGGTIDFPDDVSRTRQKLFRFLDNKFDSEKYRNNVRELTPAIL
                     AVLPLEYRGYLVEQDSFMARLAEMEKELSEAKQAVILNAPRHQKLKEISEGIVSMFRV
                     DPELAGPLMAMVTTMLGAI"
     gene            complement(1740..1962)
                     /locus_tag="ACHJWA_004817"
                     /pseudo
     CDS             complement(1740..1962)
                     /locus_tag="ACHJWA_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201115.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdaS family helix-turn-helix protein"
     gene            2073..2765
                     /locus_tag="ACHJWA_004818"
     CDS             2073..2765
                     /locus_tag="ACHJWA_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001337214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO4311235.1"
                     /translation="MKKETLADRLNLAMEQSGMSQGALAKASGVAQPTIWRLTSGNAR
                     GSTKIVEIANALGVRTEWLSSGIGPMRNDGQQSGKPAVNHSKYFKIDVLDIEVSAGPG
                     VINREFVEVLRSVEYSFDDARHMFDGRKAENIRIINVRGDSMSGTIEPGDLLFVDITV
                     KSFDGDGIYAFLYDDTAHVKRLQMMKDKLLVISDNKSYSPWDPIEKDEMNRVFIFGKV
                     IGSMPQTYRKHG"
     gene            2872..>3294
                     /locus_tag="ACHJWA_004819"
     CDS             2872..>3294
                     /locus_tag="ACHJWA_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311236.1"
                     /translation="MDMSKKNFEFKNYPIVFIGSGISKRYLENYPTWEELLNEYWKIT
                     NPTNDFYSYLLTIKESHKNNSDNDIDHKIYTEAASKIENDYNLLFRTGKLKLNGLDAK
                     RVFSEDISPFKYAICQRFSNNTIRKDVNLDELASFKTLI"
BASE COUNT          886 a          744 c          735 g          929 t
ORIGIN      
        1 ccgggaaagt tgccagtaac tggctgaaca caccgttgat gatctgcgct acctgctgta
       61 cctgcggctt ttcgtcgtac tgttccggca tgttgttggc gatccgacgc atctgctcac
      121 ggtcaaagtt aaccatctgt gcggcgatgt gtttcataga tccaccccgt aaatccagtc
      181 tgtgtttgtc aggtcgagtt ttggtttgct ggctgtcacg cctgcctgtt gcttgttacg
      241 gttgatttcg agttgggtcc acttatcgcg gagtttggcc gggctcagca cgttaccgga
      301 ccagaagttg tcctggcatg cccagcggaa cagcacgcac atgtcgcggt ggttacgtcc
      361 gtcacgttca cgcatcaggc ggatatcgtt agcccaccct gcaaaattcg gttttctggc
      421 tgatggcgcg atggtcttca ccatgtcaaa catccactct gcggcggtca ggtcttctgc
      481 tgtcccccac ttgctgccgc tctgaatcgc agcatccgct ttcaccacag gaaggtcgtt
      541 ttctggcagg tcagaggatt cgccagaatt ctcggacgaa taaggtttta tattgtcttt
      601 tgttagtttg tcttttgtgt ttacctgatt cgggtaagtg cctttacctg atttgggtaa
      661 acttttctta cctgattcag gtaaatttac ctctttcagg taaactttat ttttcttacc
      721 tgattcgggt aatgttgacc attcactgac cacattatta atgccgatat tccgcccgct
      781 ctgaataaaa atcccacgct ttaccagaac actttttgca gcagaacact tgtgcggcaa
      841 tatcccggtc aactcggaaa gttgctcgtt gctcacccag tccagttttt tattaaagcc
      901 atatgttttg cgcatgacag ccaggaagac cagaagctgg tgctgtgtta atccggccag
      961 cattacagct tccagcaact catttgcaat gcgcgtataa ccatcatcga gatctgccac
     1021 gcgcggctcc ttttgtgccg catccggcac tggaaaattg aatatctcag cagtgtttgc
     1081 cataattcct cccgcaatga gtgtgttacg atttgcacct gaaagtcggt tctgttccag
     1141 cagaccggct ttcgccattt ctgaacctgt catatcgccc ccagcatggt agtaaccatc
     1201 gccatcaatg gaccagccag ctctgggtcc acacgaaaca tcgacacaat accttcacta
     1261 atttccttca gtttctggtg gcgtggtgcg ttgagaatga cagcctgttt tgcctcactg
     1321 agttcctttt ccatttcagc caacctagcc atgaagctat cctgctcaac caggtaaccg
     1381 cgatattcca gcggtagtac cgccagaatt gccggggtca gttcacgcac gttatttcgg
     1441 tatttttcag aatcgaattt gttatcgagg aagcggaaca gcttctggcg tgtacggctg
     1501 acatcatcag ggaaatcgat ggtgccgccg ccctgctccc gatactcatt cacaatgagt
     1561 gtggcaacga catcctgatt atctacagcc gaccaggcgc ggacggcatc acggattttt
     1621 tcgtggcctg gcacctgttt tatttgagaa cgatttatca ccgcagtcgg gctaaatccg
     1681 ctagtctgtt ggtatgtaag tggttgcata atcattgcct tatcagttaa cgccgcagtt
     1741 taggcggcag aattactcgc gttaaacaat ggtgcaaggt cgggacgaat atctgctggt
     1801 ttaatctttc caccagtggc tgagacaatt ttcattacat agcgggcatc aattccgcca
     1861 ccgtgtagcc aacgccaaac agtgggctac accgcataga tctgccagtc gtttttgact
     1921 acctgtaata ctgattgcga gttgaatggt ttgatttgtc attatcaatt cctattggta
     1981 ttacaatgaa tggataatag caatgcgtat taatccaatc aatagcaaaa cgtgttttga
     2041 ccatcaatac gcaagcgtat aaattaaaac ttatgaaaaa agaaactctt gctgatcgct
     2101 taaacctagc gatggaacaa tctggaatgt ctcaaggcgc tcttgcaaag gcgtctggcg
     2161 tagctcaacc cacaatctgg agactgacaa gcggcaacgc acgcggctca acaaaaattg
     2221 ttgaaatagc taatgcattg ggtgttcgaa cagagtggct ctcatcaggc ataggcccga
     2281 tgagaaatga cggtcaacaa tcagggaagc ctgctgtcaa ccattccaaa tacttcaaga
     2341 ttgacgttct tgatatagaa gtcagtgctg ggccgggtgt catcaaccgt gagtttgtag
     2401 aagtcctacg ctcggttgag tactcgtttg acgatgctcg tcacatgttc gatggcagga
     2461 aggcagaaaa tatccgcatc attaacgtac gcggtgacag catgtcagga acgatcgaac
     2521 caggtgatct tctgttcgtt gatatcactg ttaaatcttt cgacggtgat ggtatctatg
     2581 cgtttctgta cgacgacaca gcccatgtaa agcgcctgca aatgatgaag gataagctgc
     2641 tggttatctc tgataacaag agctactcac cgtgggaccc gatcgaaaaa gacgaaatga
     2701 atcgggtatt tatatttggg aaagttattg ggagcatgcc acagacatat agaaaacatg
     2761 ggtagattat taatgacaga tttcatcaag ataaccagat gatgttttgg tgagacatgc
     2821 tgacatcctc ataatttata gaatggatgt ttcaagcaga gcttaaggta tatggatatg
     2881 tcaaaaaaaa attttgaatt caaaaattac ccaatagtct ttattggctc aggcatttca
     2941 aaaagatatt tagagaacta cccaacgtgg gaagagctat taaacgagta ttggaaaata
     3001 acaaatccca caaatgattt ctatagttat cttttaacaa taaaagaatc acataaaaat
     3061 aacagtgata atgatatcga tcataagatt tatacagagg cagcaagtaa aattgaaaat
     3121 gattacaatc tattgtttag gactggcaaa ttaaaattaa atggactaga tgccaagagg
     3181 gtttttagtg aagatatttc accatttaaa tacgccatat gccaaagatt ttctaataac
     3241 acaatcagaa aggatgtaaa tcttgatgaa ttagcatcat ttaaaacatt aata
//
LOCUS       DAZFAB010000226         3190 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZFAB010000226 DAZFAB010000000
VERSION     DAZFAB010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.227"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..242)
                     /locus_tag="ACHJWA_004820"
     CDS             complement(<1..242)
                     /locus_tag="ACHJWA_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311237.1"
                     /translation="MATQTEVARHLSLTDRQLRRLQKLPGAPISNKRGQLDLDAWRDF
                     YISYLRRSKNDVPDGDSEDDYEEKLLIARWELTAEQA"
     gene            993..1199
                     /locus_tag="ACHJWA_004821"
     CDS             993..1199
                     /locus_tag="ACHJWA_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO4311238.1"
                     /translation="MEKITTGVSYTTSAVGTEYWLLQLLDKVSPSQWVAIGVLGSLLF
                     GLLTYLTNLYFKIREDRRKVARGE"
     gene            complement(1455..1727)
                     /locus_tag="ACHJWA_004822"
     CDS             complement(1455..1727)
                     /locus_tag="ACHJWA_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311239.1"
                     /translation="MTENVKSELLLLMADNNEATSSILADPYGKISHKTLDIITTTLT
                     PLMLQRLKHNINAWVNEELSPPCLWDSRYACQQKMRIFNLLSPKLR"
     gene            1887..2420
                     /locus_tag="ACHJWA_004823"
     CDS             1887..2420
                     /locus_tag="ACHJWA_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO4311240.1"
                     /translation="MNEKIKYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWDIDR"
     gene            2641..2754
                     /locus_tag="ACHJWA_004824"
     CDS             2641..2754
                     /locus_tag="ACHJWA_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311241.1"
                     /translation="MKGIEVETPASLDLTRAAAFAIRIVAIAVLVWAIRWW"
     gene            2976..3161
                     /locus_tag="ACHJWA_004825"
     CDS             2976..3161
                     /locus_tag="ACHJWA_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO4311242.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWIMQPA
                     PDWQTPLNGIISSSENG"
BASE COUNT          849 a          693 c          814 g          834 t
ORIGIN      
        1 gcctgttctg ctgtcagttc ccatctggca ataagcaatt tctcctcata gtcgtcttcg
       61 ctatcgccat caggcacatc gtttttactt ctcctcagat acgatatgta aaaatcgcgc
      121 caggcatcca gatccagttg ccctcgctta ttcgatatcg gggcacccgg caatttctgc
      181 aatctgcgaa gctggcgatc ggtcagactt aaatgcctgg caacttcagt ctgcgtagcc
      241 actcctcacc tcgcaaaaac tctcacctca caatcacaac aaaaccggtc atgtccggtt
      301 tacatgtctg ttttttgttc atgtccggtt cacagaagac ctgtttttat atttttcata
      361 tagttaactt gaagagaaac cggacatgga tcccggaaaa ttttcataaa tagcgaaaac
      421 ccgcgaggtc gccgccccgt aacctgtcgg atcgccggaa aggacccgca aaatgataat
      481 aattatcatc tacatgtcac aacgtgcatc tacgccatca aaccacgtca aataattaat
      541 tatgacgcag gtatcgtatt aattgatctg catcaactta acgtaaaaac aacttcagac
      601 aatacaaatc agcgacactg aatacggggc aacctcatgt caacgaagaa cagaacccgc
      661 agaacaacaa ccagttacag atgctttatt aaggaaaaaa acagccagca ctgactttcg
      721 gtggagaggt gctggctcag aaggatagtt ggatttcaca tgatacttat gcctggcggt
      781 atattttctg acagacagtg acgggtgttg tcaagatatt gtgtcattta taacctgaat
      841 caggggaggc cggaatgtta tctggcattt ttagcagagc ctgaatgcca taatcacggc
      901 tcccggagtt ggccgtcagt gggtgacact ggcggctttt ttgtttttct ttactttcat
      961 tttctgtcgg cggtgacgga gacatacatc agatggaaaa aatcacaaca ggtgtgtcat
     1021 acaccacgtc agcggtgggg acggaatact ggttactgca gctgctggac aaagtctctc
     1081 cgtcccagtg ggtggcaata ggtgtgctgg gaagtctgct gtttggcctg ctgacgtatc
     1141 tgactaacct gtatttcaaa atcagagagg accgtcgtaa ggtggcgcgg ggagagtagt
     1201 cgatgaataa acaatacgaa ctggttgtaa aatgaatatt tctaactgaa aaaacgttcc
     1261 atgaggtaag aaaaggtcac aggcaatcaa taacaggacg tgatgaaaga cccttgcatt
     1321 tgtgcgcttt ctctttagat agcagcagat actgaaaatc tgagttgtcg gggagtcagg
     1381 gatacagctg tgcaagagtt ggtcattgtg attccattga aatcctgtat gccatgaagg
     1441 gcaggatttt atggctacct gagctttggt gatagtaagt tgaaaattcg cattttttgc
     1501 tgacatgcgt aacgagaatc ccataagcag ggaggactta attcttcatt aacccatgcg
     1561 ttgatattat gtttcagccg ttgaagcatc agcggtgtta atgttgtggt aataatatcc
     1621 agcgttttat gtgagatctt accgtaaggg tctgcaagaa tgctgcttgt tgcttcgtta
     1681 ttatctgcca tcagaagaag taactctgat ttaacgtttt ctgtcattag ttgtaaaaat
     1741 cttctgcgca aactttcttt actgttcatt tatatggctt catttgttgt aatctgctgc
     1801 gtctcaaggg atatgtttat gagagcgacc atgagtgttg gattatatac ctaacatatc
     1861 aagggattag aaatcgataa atccccatga acgaaaaaat aaaatacggc ctgtcggctg
     1921 ccgttctggc gctgattggt gcaggtgctt ctgcgcctga aatcctcgac cagtttctgg
     1981 atgaaaagga aggtaaccac accacggcat accgtgatgg tgcggggatc tggaccatct
     2041 gccgtggtgc cattctggtg gatggtaagc ctgttattcc tggcatgaag ctgtcaaagg
     2101 aaaaatgcga ccgggttaat gccatcgaac gtgacaaggc gctggcatgg gtggagaaaa
     2161 acatccgggt gccgctgacc gaaccccaga aagcggggat cgcgtcattc tgtccgtaca
     2221 acattggccc cggtaagtgc ttcccgtcga cgttttataa acgaattaat gcaggcgatc
     2281 gaaaaggtgc ctgtgaggcg attcgctggt ggattaagga cggtggcaga gactgccgta
     2341 tccgttcaaa taattgctac ggtcaggtct cacggcgtga ccaggagagc gcgctggcgt
     2401 gctgggacat cgacagatag cagaatattt tcctgaaaaa tgacgttggc caacgcgggt
     2461 ggataacacg aaatcctgaa aactggtaaa acctaagtga ataaaagtaa aaaccccgtt
     2521 tgttggcagc aagcggggtt ttgtgttttc tgaccttgag taaggcaagg gagaaattat
     2581 gggtagggag gtactttccc tgtgaggaag tataaaagat tctttctgag gttgtccatt
     2641 atgaaaggca ttgaagtgga gacgccagcc agtctggatt taacaagagc ggcagctttt
     2701 gccattcgta ttgtggccat tgctgttctg gtctgggcaa tccgttggtg gtgatatgaa
     2761 ccgtgttctg tgtgtggtta tcattgtcct gctggtggcc tgtggtgcgc ttagtctggg
     2821 gctgaatcat taccgtgatc acgccatcat ctacaaagag cagcgcgata aaaaagccag
     2881 tgagctggag ctggcgaacg cgacaattac tgatatgcag atacgccagc gtgatgtcgc
     2941 tgcacttgat gccagatact cgagggaatt agccgatgcg agagctgaaa atgaaactct
     3001 gcgtgctgat gttgccgctg gtcgtaagcg cctgcggatc aacgccacct gctccggtac
     3061 cgtgcgtgaa gccaccggca cctccggcgt ggataatgca accggccccc gactggcaga
     3121 caccgctgaa cgggattatt tcatcctcag agaacggctg atggcaatgc agaagcaact
     3181 ggaaggagca
//
LOCUS       DAZFAB010000227         3152 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZFAB010000227 DAZFAB010000000
VERSION     DAZFAB010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.228"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            35..1078
                     /locus_tag="ACHJWA_004826"
     CDS             35..1078
                     /locus_tag="ACHJWA_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFO4311243.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTTCHGVSLRGHIDVTTEDLASALDVTEDVIDSILHAMQGRVLDGDLISGWEKRQV
                     LKEDNGNVSQTAKSPAERKRAQREREKLRKHNADCHDESRRVTHLSRQVTTDKDTDKD
                     TDTELNPTHNARESIPTSESNGAPLQTAEPEYLDGLSEPIGKFSMTTVWQPSPDFRQR
                     AAVWGMALPEPEFTPAELAAFRDYWMAEGKVFTQVQWEQKFARHVQHVRAQVKPVSKG
                     GSHAASGGTASRAVQEIRAAREQWERDNGFISNGNGLEAVGAHGGGVFEPLDSEERGR
                     TFEALDCPDWCDD"
     gene            1071..1532
                     /locus_tag="ACHJWA_004827"
     CDS             1071..1532
                     /locus_tag="ACHJWA_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO4311244.1"
                     /translation="MTEQQIRLVCRQCMDRCRAGETWPPDLAEFVALISESGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWPQPVLYHICLEMRTRGIERQMTQGELKRLAE
                     RQLTKWAKHVGNGMSVPPVRRQLEGAKHPQGPTPIERLKQEYERRKAAGFI"
     gene            1566..2282
                     /locus_tag="ACHJWA_004828"
     CDS             1566..2282
                     /locus_tag="ACHJWA_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO4311245.1"
                     /translation="METVFDALKALKKASSQVVASRLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLTQTGESEPVSPVPKVTAQMLTEAIEHHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPGADLPAEPKAASITETDGKAFPQPAGVALPVGEAE
                     TQEEIKTESVAVTVQSQPSFTRKHPDGLILPSLHVANRELRRAKGQVQKWERVCAALR
                     ELNKCRDILRDITATREQQR"
     gene            2315..2596
                     /locus_tag="ACHJWA_004829"
     CDS             2315..2596
                     /locus_tag="ACHJWA_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO4311246.1"
                     /translation="MILRQCAGTMKVKSVGALIGRTEAAVRTKARELGISMMLRGDFH
                     PSAKYSQRDIELARQLHQRGMQRREIARKLGMPLRIVNNYVYFDRRVSA"
     gene            2593..2820
                     /locus_tag="ACHJWA_004830"
     CDS             2593..2820
                     /locus_tag="ACHJWA_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311247.1"
                     /translation="MKILYQDYGPVGQVVISSTVMEFRKHNRVVDAVLLTCPGISASR
                     AGVFIMKTKLYGSKAWIKKAYRVALQEVNSE"
     gene            2813..3124
                     /locus_tag="ACHJWA_004831"
     CDS             2813..3124
                     /locus_tag="ACHJWA_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311248.1"
                     /translation="MSEIKEMPVVRDGYGYWTHPEYEKFCDGREYISTEEFNAWMEEN
                     NLQYVLCFRDEGCADLDACDADISAWEPERPEGDGWFIGSIHDTEDGPVCVWLRNKAE
                     A"
BASE COUNT          844 a          678 c          984 g          646 t
ORIGIN      
        1 tcgagggtaa agttcggtat cagaagaggt gagtatggct aatgcctggc tcagattgtg
       61 gcatgacatg ccaaatgacc ccaagtggcg aacgattgcc agggtatcag gacagccaat
      121 cgcaacagtg atggcagtgt atatccatct tctggtgagc gcgtcacgaa atgtcacgac
      181 atgtcacggc gtgtcactac gtggtcacat tgatgtcacg acggaagatt tagcaagtgc
      241 gcttgatgtg acggaagacg taattgattc aattttgcat gcaatgcagg ggcgggttct
      301 ggatggtgac cttatttccg gatgggaaaa acgtcaggtg ctgaaagagg acaatggtaa
      361 cgtttcgcaa acggcaaaat ccccggcaga gcgcaagaga gcgcagcggg agcgggaaaa
      421 gctgcggaaa cataatgctg attgtcacga tgagtcacga cgtgtcacgc atctgtcacg
      481 acaagtcacg acagataaag atacagataa agatacagat acagaattaa accccacaca
      541 taacgcgcgc gagagtattc cgaccagtga gtcgaatggt gcgccgttgc agacagccga
      601 acctgaatac ctggacggcc tgagcgaacc gatcgggaaa ttttcgatga ctactgtctg
      661 gcagccgtcg ccggattttc gacaacgggc agcagtgtgg ggtatggctc tgcctgagcc
      721 ggaatttaca cctgctgagc ttgccgcatt ccgggattac tggatggcgg aggggaaggt
      781 tttcacgcag gttcagtggg agcagaaatt tgcccgccac gtgcagcacg tcagggcaca
      841 ggtaaaacca gtcagcaagg ggggaagcca tgcagcatca ggtggcacgg catcacgggc
      901 agttcaggaa atccgggctg cacgcgaaca gtgggaacgt gacaacggat ttatcagcaa
      961 cggaaacggc ctggaagctg tgggagctca tgggggaggt gtattcgaac cgctggactc
     1021 agaagaacgg ggccgcacct tcgaagctct ggattgccca gattggtgcg atgactgaac
     1081 agcaaatccg tctggtgtgc cgtcagtgca tggaccgctg ccgggcgggt gaaacgtggc
     1141 ccccggacct ggctgagttt gttgcgctga tttcggagag tggggcaaat ccatttggtc
     1201 ttacggtgga tgccgtgatg gaagagtacc ggcgctggcg caatgaatcc tggcgatacg
     1261 acgggagtga taaatacccg tggccacagc ctgtgctgta ccacatctgc ctcgaaatgc
     1321 gtaccagagg gattgagcgc cagatgacgc aaggtgagtt aaaacgactt gcggaacggc
     1381 aactgacgaa atgggcaaag catgttggta acgggatgag tgttccgcca gtgcgacgac
     1441 aactggaagg ggcgaaacac ccgcaagggc caacgccaat tgaacggctg aaacaggaat
     1501 acgaacgccg gaaggcagct ggttttattt gaatctgaga aacgattttg tcggaggaaa
     1561 tattaatgga aaccgtattt gacgcactga aagcactgaa aaaagcctct tcacaggtag
     1621 tggcatcgcg ccttggaatc agccgcgaag atgctgtcaa cgaactgtgg aaactgaagc
     1681 gccgtggtga agcggataac aaggggtcga tgtggtggct gactcagact ggtgaaagtg
     1741 aaccagtgtc accggtaccg aaagtgacag cgcaaatgct gactgaggcg attgaacatc
     1801 atggcccaca aacggcggat gagctggcac tgatgttcgg gattacctcc cgccgggcga
     1861 attcatcact ggccatggca atcagcaaag ggcgtctgat tcgcgtgaat cagggcggta
     1921 aatttcggta ctgcataccg ggcgctgatt taccggcaga gccgaaagcc gcatccataa
     1981 cggaaacgga tggtaaagcc tttcctcagc cagcaggtgt tgcgttacca gtcggggaag
     2041 cggaaacaca ggaagaaata aaaacggaaa gtgtggcggt cacagtgcag tcacagccgt
     2101 cgttcaccag aaagcatccg gatggtctga ttttaccatc gctgcatgtg gctaaccgcg
     2161 agctgcgccg ggcaaaaggt caggttcaga agtgggagcg agtctgcgcc gcgctgcggg
     2221 agctgaacaa gtgccgggat attctccggg atattaccgc caccagagaa cagcagcggt
     2281 gagtgggtgg aagacgtggt gccgggcgga aatcatgata ctccggcagt gtgcgggaac
     2341 gatgaaggta aaaagcgttg gcgcacttat cggacgaact gaagcggcag tgagaacgaa
     2401 ggcacgggag ctgggcatca gcatgatgtt acgtggtgat tttcacccgt cggcaaaata
     2461 ttctcagcgt gatattgagc tggcgcggca actgcatcag agaggcatgc aaagaaggga
     2521 aattgccaga aaattaggca tgccgctgcg catagtgaat aactacgttt atttcgacag
     2581 gagggtgtct gcgtgaaaat cctgtatcag gattacggcc cggtggggca ggtggttatc
     2641 agcagtactg taatggagtt tcggaagcat aaccgtgtgg tggatgctgt gctgttaacc
     2701 tgtccgggga tatcggcgag tcgtgcaggt gtgtttatta tgaagacgaa attatatggc
     2761 agtaaggcgt ggataaagaa ggcgtatcgt gtagcgttgc aggaggttaa cagtgagtga
     2821 aattaaagaa atgccggtag ttcgtgacgg atatggctac tggacacatc ctgaatatga
     2881 aaaattctgt gatggtcggg aatatatttc aacggaagag tttaacgcct ggatggagga
     2941 aaataatctt caatacgtcc tctgcttcag agatgaagga tgtgctgacc ttgatgcgtg
     3001 tgatgctgat atttctgcat gggaaccgga acgaccagag ggcgatggct ggtttattgg
     3061 ttccattcat gatacggaag atggcccggt ttgtgtctgg ttgcgaaata aggctgaagc
     3121 ataaaggcga taaaccaact aacaactaaa ta
//
LOCUS       DAZFAB010000228         3150 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZFAB010000228 DAZFAB010000000
VERSION     DAZFAB010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.229"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..619)
                     /gene="dsbA"
                     /locus_tag="ACHJWA_004832"
     CDS             complement(<1..619)
                     /gene="dsbA"
                     /locus_tag="ACHJWA_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFO4311249.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSE"
     gene            complement(636..1622)
                     /gene="srkA"
                     /locus_tag="ACHJWA_004833"
     CDS             complement(636..1622)
                     /gene="srkA"
                     /locus_tag="ACHJWA_004833"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFO4311250.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(1699..1968)
                     /locus_tag="ACHJWA_004834"
     CDS             complement(1699..1968)
                     /locus_tag="ACHJWA_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFO4311251.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            2038..2622
                     /gene="mobA"
                     /locus_tag="ACHJWA_004835"
     CDS             2038..2622
                     /gene="mobA"
                     /locus_tag="ACHJWA_004835"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFO4311252.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIAMVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            2604..3131
                     /gene="mobB"
                     /locus_tag="ACHJWA_004836"
     CDS             2604..3131
                     /gene="mobB"
                     /locus_tag="ACHJWA_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFO4311253.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQNQDG"
BASE COUNT          824 a          768 c          762 g          796 t
ORIGIN      
        1 tctcggacag atatttcact gtatcagcat actgctgaac aaaaacatcc atattgctgg
       61 tatccatacc ctgcggattc agctgatatt taccgttaac aaacatcgcc ggaacaccac
      121 gcaattgcac gtcagctgca gctttttcct gctgagcgac cagagatttc accacgaagc
      181 tgttccacgc cgcgtcgtac tcttcacctt taatacctgc gttgataaat acatcgcgga
      241 tatcagatgc tgaacgaatg gtctgggttt tctgtacgcc ttcaaacagc ggaacagtca
      301 ctttgtcttc cacgcccagc gccatcgcca cagcccatgc ctgagtcaga tctttgccca
      361 ggtcaccacc catgaagttg acgtggtatt tagtcatctt cacgccttcc ggcagttttt
      421 tcttcacatt atcagaaata tgcagaactt cttcaaactg atagcagtgc gggcagaaga
      481 aagagaaaaa ctccagcact tgcggcgcgc cagctaccgg tttttccagg gtagtgtact
      541 gtttaccatc ttcatactgc gccgccgatg cgctaaacgc taaaactaaa ccagccagcg
      601 ccagccaaat ctttttcatg atctactctc tccgattaat acataggtgt taattgcaaa
      661 gggggttctt gtagaacttt tgcctgttct ataaaagtcg ccgtctgtcg tagccagtaa
      721 tcttccccgg ttaaccacgg gaaatttttc gggaacgcgg gatcagccca acgccgcatt
      781 agccaggcaa gataataaac caaacgcatg gcgcgtaaag gttcaatcag tccgatttca
      841 gcggtgtcga actcgctaaa ttcttcataa gcttcaataa tagtttccag ttgcatccgc
      901 tgctcggctt tatcgccatt gagcaacatc cacaaatcct gaatagctgg accattacgt
      961 gcatcatcca gatcaacaaa cattggacca tcgcgccaga gaatattccc ggcgtggcag
     1021 tctccatgta gccgcagaac ggtgaaatct tcccgccagt gtgctgtaac ggcggcgatc
     1081 agctcatctg tcgctttcag gaatgccgct ttcaacccgg aaggtatcag tgtagcgtcc
     1141 tcaaacagct tgcgtggctc aatgagatat tcattcagac cgatggtcgg gcgatggata
     1201 aaaagctgtt tgcgccccgt ctggtgcata cgccctaaat aacgcccaac cgcttccatc
     1261 tgatcgatat tatcagcttc gaactggcga ccaccgacgc ttggaaaaac agcgaaataa
     1321 aatccctgat gattcaataa agtctgaccg ttaaaagcca caggtgctgc aaccggaact
     1381 tcatcattta ccagctgcaa cgcaaattga tgttcttcga ggatttgatc ggctgtccaa
     1441 cgttcaggac gataaaattt gacgacaaaa cgtcgacgat cttcgtcctg aaattgatag
     1501 acacggtttt catagctgtt aagcggggta agaccggaat ccacccggat cccttgctca
     1561 aacagagcgt ccatgatggt atccgggtgt agtgtctgga aagtaaaagc gctgttgttc
     1621 atcccatcat ccggaaaaca ttacgaatga tgaaggatat catttcacga tgattttggt
     1681 ggcgccgctt acaagctttt actctttaat tacgccgcga gcgcgtaaca gcgccgtttt
     1741 gaagtcttct tcataatctt tctgcaaacc cgggatcacc gcatctttcg cggaatcacg
     1801 cattttcagg tgatagatca gaatgtcatc cgtcaaatcc gccagttcgc cgtcaaaacc
     1861 tgactctttc gccagtttct gcaaaaattg caacaggtta aggtctggct ctttttgcca
     1921 ggctggctgg aggagttcaa taacttcatt cagacgttta catttcatag taatgctcct
     1981 tactcttgag acagacacgt tagcagggtc aatcccacaa taaaagaggc gatatcggtg
     2041 aatctgatga cgacgataac aggcgttgtg ctggcaggcg gtaaagccag acgaatgggc
     2101 ggcgtagata aaggattgct ggacttaaat ggcaaaccgc tgtggcaaca tgtcgctgac
     2161 gcgcttatga cgcagctctc tcacgtcgtg gttaatgcta atcgtcatca ggaaatctat
     2221 caggcaggcg gtctgaaagt gattgaagat tcactggcgg attacccagg ccctctggca
     2281 gggatgcttt cagtaatgca gcaggaagct ggtgagtggt ttttgttttg tccgtgcgat
     2341 acgccttaca ttccccatga tttagccgcc cggcttaatc atcagcgcaa agatgcgcct
     2401 gtcgtgtggg tccatgacgg tgaacgcgat cacccgacta ttgctatggt aaaccgcgct
     2461 attgagcctt tattactaga atatctgcaa gcaggagaac gccgggtaat ggcatttatg
     2521 cgtctggctg gcggtcatgc ggttgatttc agcgatcata aagatgcatt tgttaacgtg
     2581 aatacgccag aggagcttgc ccgatggcag gaaaaacgat gataccgtta ctcgcctttg
     2641 ccgcgtggag tggtaccgga aaaactacgc ttttgaaaaa actgatcccg gcattatgcg
     2701 ccagagggat ccgtccaggg ctgattaagc atacgcacca tgatatggat gttgataagc
     2761 caggcaaaga tagctatgag ctgcgcaagg ctggcgcggc acaaaccatc gttgccagcc
     2821 agcaacgatg ggccttgatg acggaaacac cagacgaaga agagctggat ctacagtttc
     2881 tcgcaagtcg aatggatacc tcaaagctgg atttgattct ggtcgaaggg tttaagcatg
     2941 aagagatcgc aaagattgtg ctgtttcgcg atggagccgg acatcgacct gaagaattag
     3001 tgatagacag gcatgttatt gctgtagcca gtgatgtgcc gcttaatctt gatgtcgcat
     3061 tactggatat taatgatgtt gaggggctgg ctgattttgt ggtggagtgg atgcaaaatc
     3121 aggacggata agcaagagta ggctggagca
//
LOCUS       DAZFAB010000229         3044 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZFAB010000229 DAZFAB010000000
VERSION     DAZFAB010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.230"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            559..765
                     /locus_tag="ACHJWA_004837"
     CDS             559..765
                     /locus_tag="ACHJWA_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HFO4311254.1"
                     /translation="MVHQHYGTQTVNRGAVMPGMLVKHKDGTWTASANLRGRLYLHRG
                     IERTYTRDLLVEVFLDGRGNGLNR"
     gene            841..1137
                     /locus_tag="ACHJWA_004838"
     CDS             841..1137
                     /locus_tag="ACHJWA_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO4311255.1"
                     /translation="MNAYYIQDCLEAQSWARYYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            1143..1928
                     /gene="bet"
                     /locus_tag="ACHJWA_004839"
     CDS             1143..1928
                     /gene="bet"
                     /locus_tag="ACHJWA_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFO4311256.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            1925..2602
                     /locus_tag="ACHJWA_004840"
     CDS             1925..2602
                     /locus_tag="ACHJWA_004840"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFO4311257.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEIHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVIERNEKYMASFDEMVPEFIEKMDEALAEIG
                     FVYGEQW"
     gene            2602..2784
                     /locus_tag="ACHJWA_004841"
     CDS             2602..2784
                     /locus_tag="ACHJWA_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFO4311258.1"
                     /translation="MKHPHDNIRVGTITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEE
                     INNKRGAVCTKHLLLN"
     gene            2757..2948
                     /locus_tag="ACHJWA_004842"
     CDS             2757..2948
                     /locus_tag="ACHJWA_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFO4311259.1"
                     /translation="MHKASPVELRTSIGMAHSLAQIGVRFVPIPVETDEEFHTLATSL
                     SQKLEMMAAKAEANERDPA"
BASE COUNT          839 a          750 c          750 g          705 t
ORIGIN      
        1 ggctaataag tcatttatgt atcatttagc aactgactaa aaaaatacac ataagaactc
       61 agactaccaa tattcgatta aatttgctgc caacctaccg agttcaccac aacccgatgc
      121 ccctctgtca cgaggcacat taaagctgta caaatttcag ccacaccaat aagtcttcat
      181 actctttttt ataaaaattc atttaattat caatcagaac aagtatatca aacattatca
      241 atactaattg ctattgacca cacaatagcc attgctattg tcatgctatt gcatcgatgt
      301 tggtatcaca aaatttataa cttcaccgtt gcgatgaccg cttagatccg cagcttgaat
      361 ttcagcaggc tccggggagt gcgaggggtg aagcggacgc gtgaacgtcg gtgtgaccag
      421 ctgaaatcaa ctcaacactt catacctcag tcgcttcaac gaggcgactt agttatgaca
      481 accggcggcc atccaccgcc tgaatacgcg cagaagtctc tatatgttca gcagcccagc
      541 ttacgggcag gagtttttat ggttcatcaa cattacggaa cgcagaccgt taatcgaggt
      601 gcggtcatgc caggaatgct ggtcaaacac aaagatggta cctggactgc atcagctaat
      661 ttacgcggac ggctttatct gcatcgcggc atcgagcgca cttatacccg tgatttgctc
      721 gtggaagttt ttctcgacgg acgcggtaac ggcctgaatc gctaatcccc tttcctgttt
      781 tcctaatcag cctggcattt cgcgggcgat attttcacag ccattttcag gagttcagcc
      841 atgaacgctt attacattca ggattgtctt gaggctcaga gctgggcgcg ttactaccag
      901 cagatcgccc gtgaagagaa agaggcagaa ctggcagacg acatggaaaa aggcctgccc
      961 cagcacctgt ttgaatcgct atgcatcgat catttgcaac gccacggggc cagcaaaaaa
     1021 gccattaccc gtgcgtttga tgacgatgtt gagtttcagg agcgcatggc agaacacatc
     1081 cggtacatgg ttgaaaccat tgctcaccat caggttgata ttgattcaga ggtataaaac
     1141 ggatgagtac agcactcgca acgctggctg ggaagctggc tgaacgtgtc ggcatggatt
     1201 ctgtcgaccc acaggaactg atcaccactc ttcgccagac ggcatttaaa ggcgatgcca
     1261 gcgatgcgca gttcatcgca ttgttgatcg tcgccaacca gtacggcctt aatccgtgga
     1321 cgaaagaaat ttacgccttc cctgataagc agaacggcat cgttccggtg gtgggcgttg
     1381 atggctggtc ccgcatcatc aacgaaaacc agcagtttga tggcatggac tttgagcagg
     1441 acaatgaatc ctgcacatgc cggatttacc gcaaggaccg taatcatccg atctgcgtta
     1501 ccgagtggat ggatgaatgc cgccgcgaac cattcaaaac ccgcgaaggc agagaaatca
     1561 cggggccgtg gcagtcgcat cccaaacgga tgttacgtca taaagccatg attcagtgtg
     1621 cccgtctcgc cttcggattt gctggtatct atgacaagga tgaagccgag cgcattgtcg
     1681 aaaataccgc atacactgca gaacgtcagc cggaacgcga catcactccg gttaacgatg
     1741 aaaccatgca ggaaattaac actctgctga tcgccctgga taaaacatgg gatgacgact
     1801 tattgccgct ctgttcccag atatttcgcc gcgacattcg tgcatcgtca gaactgacac
     1861 aggccgaagc agtaaaagct cttggattcc tgaaacaaaa agccactgaa cagaaggtgg
     1921 cagcatgaca ccggacatta tcctgcagcg taccgggatc gacgtgagag ctgtcgaaca
     1981 gggagatgat gcgtggcaca aattacggct cggcgtcatc acagcttcag aaattcacaa
     2041 cgtaatagca aaaccccgat caggaaagaa gtggcctgac atgaaaatgt cctacttcca
     2101 caccctgctg gctgaggttt gcaccggtgt ggctccggaa gttaacgcta aggctctggc
     2161 ctggggaaaa cagtacgaga acgacgccag aaccctcttt gagttcactt ccggcgtgaa
     2221 tgttactgaa tccccgatca tctatcgcga cgaaagtatg cgcaccgcct gctctcccga
     2281 tggtttatgc agtgacggca acggccttga gctgaaatgc ccgtttacct cccgggattt
     2341 catgaagttc cggctcggtg gtttcgaggc cataaaatcg gcttacatgg cccaggtgca
     2401 gtacagcatg tgggtgacac gaaaagatgc ctggtacttt gccaactatg acccgcgcat
     2461 gaagcgtgaa ggcctgcatt atgtcgtgat tgagcggaat gaaaagtaca tggcgagttt
     2521 tgacgagatg gtgccggagt tcatcgaaaa aatggacgag gcactggctg aaattggttt
     2581 tgtatatggg gagcaatggt aatgaagcat cctcacgata atatccgggt aggcacgatc
     2641 actttcgtct actccgttac aaagcgaggc tgggtatttc ccggcctttc tgttatccga
     2701 aatccactga aagcacagcg gctggctgaa gagataaata ataaacgagg ggctgtatgc
     2761 acaaagcatc tcctgttgaa ttaagaacga gtatcgggat ggcacatagc ctcgctcaaa
     2821 ttggagtcag gtttgtgcca ataccagtag aaacagacga agaatttcat acgttagcca
     2881 catccctttc acaaaagctg gaaatgatgg cggcgaaagc agaagcaaac gagagagacc
     2941 cggcatgaca acaacagaat gcatttttct ggcagcaggc ttcatattct gtgtgcttat
     3001 gcttgccgac atgggacttg ttcaatgaca cctcagcagg aaaa
//
LOCUS       DAZFAB010000230         2937 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZFAB010000230 DAZFAB010000000
VERSION     DAZFAB010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.231"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..286)
                     /locus_tag="ACHJWA_004843"
     CDS             complement(<1..286)
                     /locus_tag="ACHJWA_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO4311260.1"
                     /translation="MTVKISGVLKDGTGKPVQNCTIVLKARRTSSTVVVNTVASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMSE"
     gene            complement(438..1037)
                     /locus_tag="ACHJWA_004844"
     CDS             complement(438..1037)
                     /locus_tag="ACHJWA_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO4311261.1"
                     /translation="MRKLCAVILSAVVWQVAAATPASAAEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFMDALGLITSFSYANAEDEQKTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFYGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVTIDLAYEGSGSGDWRSDAFIVGIGYRF"
     gene            complement(1105..>2937)
                     /locus_tag="ACHJWA_004845"
     CDS             complement(1105..>2937)
                     /locus_tag="ACHJWA_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO4311262.1"
                     /translation="TLPDGVETYGVWGLSLPSLRRRLFRCVSVRENTDGTFAITAVQH
                     VPEKEAIVDNGARFEPQSGSLNSVIPPAVQHLTVEVSAADGQYLAQAKWDTPRVVKGV
                     RFSLRLTSGKGTDARLVTTAITADTEHRFSGLPLGEYTLTVRAINSYGQQGEPATTTF
                     RIAAPAAPSRIELTPGYFQITATPHLAVYDPTVQFEFWFSEKRIADIRQVETAARYLG
                     SALYWIAASINIKPGHDYYFYIRSVNTVGKSAFVEAVGRASDDAEGYLDFFKGEIGKT
                     HLAQELWTQIDNGQLAPDLAEIRTSITNVSNEITQTVNKKLENQSAAIQQIQKVQVDT
                     NNNLNSMWAVKLQQMQDGRLYIAGIGAGIENTPAGMQSQVLLAADRIAMINPANGNTK
                     PMFVGQGDQIFMNEVFLKYLTAPTITSGGNPPAFSLTPDGRLTAKNADISGNVNANSG
                     TLNNVTINENCRVLGKLSANQIEGDLVKTVGKAFPRDSRAPERWPSGTITVRVYDDQP
                     FDRQIVIPAVAFSGAKHEKEHTDIYSSCRLIVRKNGAEIYNRTALDNTLIYSGVIDMP
                     AGHGHMTLEFSVSAWLVNNWYPTASISDLLVVVMKKATAGITIS"
BASE COUNT          666 a          858 c          709 g          704 t
ORIGIN      
        1 cctccgacat ggcaccgaga aaatcattca gcgtaccggg ttgagaatct tcatacacgg
       61 tgatggtccc ggcatgtgac ggaggaaatc cctccaccaa cagaatgacg ctgtactgac
      121 cgtactcaac atccatgctg taacgaccgg cttcatccgg attttcagag gccaccgtgt
      181 tcaccaccac cgtgctgctg gttcgtctgg ccttcagcac aatggtgcag ttctgtactg
      241 gttttcctgt gccatcttta agcacgccag aaatttttac tgtcatactt ttccaccaat
      301 aaaaaaagcc cgcagcagtg acgccacggg cttcaggaca gtgtaacttt acgtttcctc
      361 aaacgcagtt caccccataa ggtggatgaa cctgcgtatc ataacaatat ttacagaaga
      421 taaatcggcg tctgttgtca gaaacggtat ccgataccaa caataaatgc atccgatcgc
      481 cagtcgccac taccggaacc ttcataagca aggtcaatgg tcacggattc ggtcgggtta
      541 aactgcacgc cagcccccca cgccagagac gtgttgctgt ggcgaccgtc atcacttccg
      601 gtcagcacat cgtgcgtttt ccccttgttg tcagttacgc ggagataatc cccgtagaaa
      661 gtcgacacac ggctgtaagc cacacccgcc atcgcatacg cgctgaacca ttcattcacg
      721 cgtacagacg gccccgccat cacgctgaac cagcggttac gcacggaatc ttcatgccag
      781 cgggtatcgc tgtagcgcgt tttttgctca tcctcagcat tggcataact gaaggacgta
      841 atcagcccca gcgcgtccat aaactcataa cggtatttca cgttaatccc gttcagatca
      901 tcactaccgg gaacgttcgt cgaggcatgg agataccccg cgctcagcgt ggactgatgt
      961 tctgctgcac tcgctggcgt agcagcggcg acctgccaga ctactgcgga caaaataaca
     1021 gcacataatt tacgcataat tacctctcgc ttttctgcaa taaaaaaggc gccatttctg
     1081 gcgcccgtat ctgggttata aaattcagct aatcgtgatg cctgcagtgg ctttcttcat
     1141 caccacaacc agcaaatcgc tgatacttgc tgtgggatac cagttattta ccagccatgc
     1201 tgacaccgaa aactccagtg tcatgtgacc gtgaccggca ggcatatcaa taacaccact
     1261 gtaaatcagc gtattatcca gcgcggtacg gttataaatt tcagcaccgt ttttccgcac
     1321 tatcagacgg catgaggagt aaatatcagt atgctctttc tcatgtttag cgccgctgaa
     1381 tgccaccgcc ggaataacaa tctgccggtc aaacggctga tcgtcataaa ccctgacggt
     1441 aatggttcct gatggccacc gctccggtgc acgggagtcc cgggggaaag ctttgcccac
     1501 tgttttaacg agatcgcctt caatctggtt cgcggacaat tttcccagaa cccgacagtt
     1561 ctcgttaatc gtgacgttgt tgagcgtccc ggagttcgca ttcacgttac cgctgatatc
     1621 ggcatttttc gccgtcagcc gcccgtccgg tgtcagggaa aatgccggag gattaccgcc
     1681 gctggtaatg gtgggagccg tcagatattt caggaacact tcattcataa atatctgatc
     1741 gccctgacca acaaacatcg gctttgtgtt gccattcgca ggattaatca tcgcaatcct
     1801 gtctgccgcc agcagcacct gactctgcat tcctgctggc gtattctcaa taccggcacc
     1861 gatacccgca atataaaggc gtccgtcctg catctgctgc agcttcactg cccacatgct
     1921 gttcaggtta ttatttgtat caacctgaac tttctgtatc tgctggattg ccgcactctg
     1981 attttccagt tttttattga cggtctgcgt gatttcattg ctgacattcg taatggacgt
     2041 cctgatttca gccaggtccg gcgcaagctg accgttatca atctgcgtcc acagctcctg
     2101 ggccagatgt gttttcccga tttctccttt gaaaaaatcc aggtaacctt ccgcatcatc
     2161 gctcgcccga ccgacagcct ccacgaatgc cgatttgcca acagtattca cactgcggat
     2221 ataaaaataa taatcatggc ccggtttgat attgatactg gcagctatcc agtacagcgc
     2281 cgagccaaga tagcgggctg cggtttcaac ctgcctgata tccgcaatcc gcttttccga
     2341 gaaccagaac tcaaactgta ccgtcgggtc ataaacggca agatgcggcg tggcggttat
     2401 ctgaaaatag cccggcgtca gctcaatccg cgacggtgct gccggtgcgg caatccggaa
     2461 ggtggtggtg gcaggttcac cctgctggcc atagctgttt atcgcccgca ccgtcagggt
     2521 gtattccccg agcggcaggc cgctgaaacg gtgctccgtg tctgcggtga tggcggtggt
     2581 caccagtctg gcatccgttc ccttaccact ggtcaggcgc agactgaagc gcacgccctt
     2641 caccacccgc ggcgtgtccc atttagcctg cgccagatac tggccgtcag ctgcactcac
     2701 ctccaccgtc aggtgctgta ctgccggtgg gatgacgctg ttcagggaac ctgactgcgg
     2761 ctcaaagcgg gcaccgttat ccacgatggc ttctttttcc ggtacgtgct gcaccgccgt
     2821 gatggcaaag gtgccgtccg tgttttcccg gacggagaca cagcggaaca ggcgacggcg
     2881 cagtgacggc agggagagtc cccacacccc gtatgtctcc acaccatcag gcagggt
//
LOCUS       DAZFAB010000231         2881 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZFAB010000231 DAZFAB010000000
VERSION     DAZFAB010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.232"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            101..400
                     /locus_tag="ACHJWA_004846"
     CDS             101..400
                     /locus_tag="ACHJWA_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311263.1"
                     /translation="MALYIDISAIAGQVRVIRAVTKRYAPLLQKVSDECTEDIVNDFV
                     IELRGLIFSYKVTTIFADGSRETVRALRLKGCVKDFATTFWARKLDCIHNQFPLE"
     gene            472..690
                     /gene="ydfC"
                     /locus_tag="ACHJWA_004847"
     CDS             472..690
                     /gene="ydfC"
                     /locus_tag="ACHJWA_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFO4311264.1"
                     /translation="MQKREPVIIAPDYTDDELYEWMHQKINAAQDLKWANEARAKQAE
                     NLSALEQDITNLEKAAALSIARMITYPR"
     gene            1259..1447
                     /gene="dicB"
                     /locus_tag="ACHJWA_004848"
     CDS             1259..1447
                     /gene="dicB"
                     /locus_tag="ACHJWA_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO4311265.1"
                     /translation="METLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKHERELLNKI
                     CILSMLARLRPIQKGCWQ"
     gene            1444..1635
                     /locus_tag="ACHJWA_004849"
     CDS             1444..1635
                     /locus_tag="ACHJWA_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO4311266.1"
                     /translation="MNTAFALVLTVFLVSGEPVDIAVSVHRTMQECVTAATEQKIPGN
                     CYPVDKVIHQDNNEIPAGL"
     gene            1728..>2881
                     /locus_tag="ACHJWA_004850"
     CDS             1728..>2881
                     /locus_tag="ACHJWA_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO4311267.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFLVCEDKPGIPRPALDSWDAEYMQENRWDEGAASFVPVETESDPM
                     NVAFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCKPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     RVHRQHTENSAEKIPPVTAVIRREYKQTWKTLDDEQAYALWPGDVDAGNIDGSIHRWA
                     KNEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYITEY
                     LTTKGVFEDEGTNQSATDTLSSPVPETDAVETAIPDNEKTECKVEVEPSV"
BASE COUNT          826 a          670 c          722 g          663 t
ORIGIN      
        1 tgttccgcta ccccggcgtt aaggggaaac agagggtttc tcagtgggcg aagtcaaaca
       61 tcagaatgga aggcatcccg ggatcggcaa agaagcagca atggcgcttt atattgacat
      121 cagcgccatt gcaggacagg taagagttat cagagcggta actaagcggt atgcgccttt
      181 acttcagaaa gtctctgatg agtgcaccga agatattgtc aacgatttcg tcatcgaact
      241 gcgaggactc atcttcagtt acaaggtgac cacaattttt gcagatggct cccgcgaaac
      301 tgtcagagcc ctgcggctta aaggatgtgt caaagacttc gccaccacat tctgggcaag
      361 aaaacttgat tgtattcata accaatttcc tctcgagtaa cagacccctc agaggatacc
      421 acctcgcctg acgtggttaa aagcaggcaa cgctaaccac aaggagccga catgcagaaa
      481 cgagaacccg tcatcatcgc gccagactat accgatgatg aactttatga gtggatgcac
      541 cagaaaatta atgcagcgca ggatctgaaa tgggccaatg aagccagggc taagcaggct
      601 gaaaatctgt ccgctctgga gcaggatatc accaatctgg aaaaagcagc ggcattaagc
      661 attgccagaa tgattacata cccgcgttaa tagctaacca acgaagctaa ggttggtaat
      721 taaggagttc tccacgggtg aggtggagtg cgtgcgccgg acacgggtga gcatacggca
      781 ctgacagttt actgaaagga tatttccctg aaaagtcaga ccataacgcg aaagcgcacg
      841 gcgaggtagc tggttcatag atagcctgtc gttaaatttt cgtcgaccgt gcgcttccgg
      901 ttgtggcaat ccgcgaaatg gcgcggcggt aagtatggag gggttattcc ttcccccgtt
      961 gaggacaccg ggttgtcagg ttgaccatac gcttaagtga caaccccgct gcaacgccct
     1021 ctgttatcaa ttttctggtg acgtttggcg gtatcagttt tactccgtga ctgctctgcc
     1081 gcccttttta aagtgaattt tgtgatgcgg tgaatgcggc tgagcgcacg cggaacagtt
     1141 aaaaccaaaa acagtgttat ggggggattc tctgtatccg gcgttaattg ttaactggtt
     1201 aacgtcacct ggaggcacca ggcaccgcat cacaaaattc attgttgagg acgcgataat
     1261 ggaaacgtta ttaccaaacg ttaatacgtc tgaaggttgt tttgaaattg gtgtcactat
     1321 cagtaaccct gtatttactg aagatgccat taacaagaga aaacacgaac gggagctatt
     1381 aaataaaata tgcattcttt caatgttggc ccgtttacgt ccgatacaaa aaggatgctg
     1441 gcaatgaata cagcatttgc acttgttctg acagtttttc ttgtttccgg agagccagtt
     1501 gatattgcag tcagtgttca caggacaatg caggagtgtg tgactgcagc aaccgaacag
     1561 aaaattcccg gtaactgtta cccggtcgat aaagttattc accaggataa taacgaaatc
     1621 ccggcaggtc tttaaaacag ttccgtaata aacatccgat ttcattctta tatgccagca
     1681 atggcaggga tttgttcacc cttaaatctg taatgaggta aaacaaaatg agtaaagtct
     1741 ttatttgcgc cgccattccg gacgaacagg caataaagga agaaggtgca gtcgctgtag
     1801 ccactgccat tgaagccggt gatgaacgtc gcgcccgcgc aaaatttcac tggcaattcc
     1861 tggagcatta tccggctgct caggactgcg cttataaatt tcttgtttgc gaggataaac
     1921 ccggtatacc ccgccctgcc ctcgattcct gggatgctga atatatgcag gaaaaccgct
     1981 gggatgaggg ggctgcttcc tttgtcccgg ttgagactga atccgatccg atgaacgtcg
     2041 cttttgacaa gctggcccct gaagtacaga acgctgtcat ggttaagttc gacacatgtg
     2101 aaaacatcac cgttgatatg gtgattagcg cgcaggaatt gttgcaggaa gacatggcaa
     2161 cattcgacgg acatatcgtt gaagcgttga tgaaaatgcc agaagttaac gccatgtatc
     2221 cggagcttaa gctgcatgcc atcgggtggg ttaagcataa atgtaagcct ggtgccaaat
     2281 ggcccgaaat tcaggcagaa atgcgcatct ggaaaaaacg tcgcgaaggt gaacgcaagg
     2341 aaaccggaaa atacacgtct gttgttgatc tcgcccgcgc cagagtccac cgacagcaca
     2401 ctgaaaactc agcagaaaaa atcccccctg tcactgcagt cattcgtcgc gaatataagc
     2461 agacatggaa aacactggat gacgaacagg cctacgctct ctggcctggt gatgtggatg
     2521 ccggaaacat tgacggcagc atccatcgct gggcaaaaaa tgaagttatc gacaacgacc
     2581 gcgaagactg gaagcgtatc tcggcatcaa tgcgcaaaca gcctgatgcc cttcgctacg
     2641 accgccagac tatttttggc cttgtccgtg aacgtccgat cgacattcac aaagaccctg
     2701 tggcactgaa caaatacatt actgaatacc tgactacaaa gggcgtgttt gaagatgaag
     2761 gaacaaatca gagcgcaact gacactctct cgtcgccagt accagaaact gatgcagtgg
     2821 aaacggcaat tccggacaac gaaaaaaccg aatgcaaagt ggaagtcgaa ccatctgtag
     2881 a
//
LOCUS       DAZFAB010000232         2820 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZFAB010000232 DAZFAB010000000
VERSION     DAZFAB010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.233"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..1890
                     /locus_tag="ACHJWA_004851"
     CDS             <1..1890
                     /locus_tag="ACHJWA_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO4311268.1"
                     /translation="LAEKLTHKLKEGWQPFGSPVAITPYTLMQAIAAEGDVTTPVVVP
                     GTGDGGYPGVVTTEPDYYYVIPLAGQSNGMAYGEGLPLPQTYDRPDPRIKQLARRSTV
                     TPDGAPCKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGTVGQGLHIAKKLLPFIP
                     ANAGILLVPCCRGGSAFTTGADGTYSDVTGASESSTRWGVGRPLYKDLIGRTKAALAK
                     NPKNVLLAVVWMQGEFDFDGTPANHTARFTEVVEQYRTDLADMVGQCAGGSADGVPWI
                     CGDTTYFWKQKSESTYQTVYGSYKNKTEKNIHFVPFMTDENGANVPTNKPEEDPDIPA
                     SGYYGAASRTSANWTSADRASHFSSWARRGIISDRLASAILLHAGRTAELVGGEQVVM
                     PPDEKPSPDTPSTPSTDGKSVTTLLYYRATESGGLLNPQGWGAEGGRALVVDDAGAAG
                     GKALRWTKQTGSSSWFMQHDAGNGADLLEKGGLISCRFKVDGTLTANQYALALYWPVS
                     SLPQGVTREGNAGHNLLASFYVQSDATDLNVMYHKGNAGQNTKLGSFGAFDNEWHTLG
                     FRFAGNNSIEVTPVIDGKDGTPFMLSQSPVGTFTADKLRVTDITSGATYPVLIESITV
                     EVNNP"
     gene            2038..2220
                     /locus_tag="ACHJWA_004852"
     CDS             2038..2220
                     /locus_tag="ACHJWA_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO4311269.1"
                     /translation="MRFVQLILLYFCTVVCTLYLVSGGYKVIRNYIRKKIDAAAAEKI
                     SASQSAGTKPEEPLIS"
     gene            2258..2527
                     /locus_tag="ACHJWA_004853"
     CDS             2258..2527
                     /locus_tag="ACHJWA_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO4311270.1"
                     /translation="MSEIKSLVTAEAVKDVLRSEEVRSALKQQLRQNLEARLDAEVDA
                     ILDELLGGPAAPEPEDGAGDSAVSDGVVSQPDGSSEPQPGGEMMM"
     gene            2603..2818
                     /locus_tag="ACHJWA_004854"
     CDS             2603..2818
                     /locus_tag="ACHJWA_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO4311271.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
BASE COUNT          701 a          661 c          850 g          608 t
ORIGIN      
        1 cttgcggaaa agctgacaca caaactgaaa gagggctggc agccgtttgg tagtcctgtg
       61 gccataaccc cttataccct gatgcaggcg attgccgcgg agggggatgt gaccacgcca
      121 gtggttgtgc ccggcacggg ggatggtggc tatccgggag tggtcaccac ggagccagat
      181 tattactacg ttattccact ggccgggcag tcgaacggca tggcttacgg tgaggggctt
      241 cctctgccgc agacatatga ccgtcctgac ccgcgtataa agcaactggc tcgtcgcagc
      301 acagtgacgc cggatggcgc tccctgtaaa tataacgaca ttattccggc agaccactgt
      361 ctgcatgatg tacaggacat gagccgtctt aaccatccga aagctgacct gtcgaaaggt
      421 cagtacggaa ccgtggggca ggggctgcat attgccaaaa agctgctgcc gtttataccg
      481 gcgaatgcgg gtattcttct ggttccgtgc tgccgtggtg gttcagcttt caccaccggg
      541 gcagatggaa catacagtga cgtgaccggt gcctcagaga gttctacccg ctggggtgtg
      601 ggcaggccgc tgtataagga tctcatcggt cgtacaaaag ccgcgctggc aaagaacccg
      661 aaaaatgtgc tgcttgccgt ggtgtggatg cagggggaat ttgactttga cggaacgcca
      721 gcaaatcaca cagcccgttt tacagaagta gtggaacaat atcgtacgga ccttgcagat
      781 atggtgggac agtgcgctgg tggttctgct gacggtgttc cctggatatg tggagacaca
      841 acttatttct ggaagcagaa gagcgaatcc acttaccaga cggtgtacgg cagttacaaa
      901 aacaaaacgg aaaagaatat tcactttgtg ccgttcatga ccgatgagaa cggagcaaat
      961 gtcccgacga acaaaccgga agaagacccg gatattccgg catcaggata ttacggtgcg
     1021 gcctcccgga cgtcggcaaa ctggacgtca gcagaccgtg cgagccattt cagctcatgg
     1081 gcacgcaggg ggattatttc tgaccgtctt gcctcagcga ttcttctcca tgcaggacgg
     1141 acggctgaac tggtgggtgg ggaacaggtt gtgatgccgc cggatgagaa gccgtcaccg
     1201 gacacaccat caacaccgtc aacggacggg aaatcagtga caacgctgct ttattaccgt
     1261 gcaacagagt caggtggttt actgaatccg cagggatggg gagctgaagg agggcgtgca
     1321 ttggtagttg atgatgcagg tgctgcagga ggtaaggcgc tgaggtggac caaacagaca
     1381 ggaagttcct cgtggtttat gcagcatgat gccggtaatg gcgcagacct gctggagaag
     1441 ggcgggctta tcagttgtcg ttttaaagtt gatggcacac tgacagctaa tcagtacgca
     1501 ctggcgctgt actggccggt ttcttcactg cctcagggcg tcacacggga aggtaatgcc
     1561 ggtcataacc tgctggcgtc gttttacgta cagagcgatg ccacagacct taatgtgatg
     1621 taccacaagg gaaatgctgg tcagaacacg aagctggggt cattcggcgc atttgataac
     1681 gaatggcata cgctgggctt ccgttttgcc ggtaacaaca gtattgaggt gacgccggtc
     1741 attgatggta aggacgggac gccgttcatg ctgtcacagt caccggtcgg cacgtttacg
     1801 gcagacaaat tgcgcgtgac cgatatcact agcggtgcga catatccggt gctgattgaa
     1861 agtataacag tggaagtgaa taacccgtaa gcaggaaaaa aaggccgccg gggcagggaa
     1921 aacaaggagc cagaaccggc ggcaaatgtc gttatatcca aagcaaaaca tgcaggacac
     1981 ttttttaacc aacaggtatt aacgatgtca acaccatatc aataaccggg agggataatg
     2041 agatttgtac agcttatttt attgtatttc tgcacggtgg tgtgcacgtt atatctggta
     2101 agtggcgggt ataaggttat ccggaactat atacgcaaaa agattgatgc cgcggcggcg
     2161 gaaaaaatca gcgccagcca gtcagccgga acaaaacccg aagagcctct catttcgtag
     2221 caactttctt aacaacacct ttcaacgaga aaatcccatg tcagaaataa aatctctggt
     2281 cactgctgaa gcagtgaagg acgtcctgcg ctctgaagaa gtcagaagcg cactgaaaca
     2341 gcaactccgc cagaatcttg aggcgcgtct tgatgctgaa gtggatgcca ttctggatga
     2401 gctgctgggg ggaccggctg ctcctgagcc tgaagacggc gcgggtgaca gtgctgtttc
     2461 agatggcgtt gtgtctcagc ctgacggtag cagtgagcct cagcctggcg gcgaaatgat
     2521 gatgtaacca tacgcagggg ctgtcggtgt gagctgatgc ccctcctgtt gttgtgagct
     2581 tccggattgc gggagacggg gtatgtacca gatggaaaaa atcacaacgg gtgtgtcata
     2641 caccacgtca gcggtgggga cgggatactg gttactgcag ctgctggaca aagtctctcc
     2701 gtcccagtgg gtggcgatag gtgtgctggg gagtctgctg tttggcctgc tgacgtatct
     2761 gactaacctg tatttcaaaa tcagagagga ccgtcgtaag gcggcgcggg gagagtaaag
//
LOCUS       DAZFAB010000233         2801 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZFAB010000233 DAZFAB010000000
VERSION     DAZFAB010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.234"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..570
                     /locus_tag="ACHJWA_004855"
     CDS             <1..570
                     /locus_tag="ACHJWA_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO4311272.1"
                     /translation="KTGAEAIRALATQLPVFRQKLSDGWYQVRITGRDVSTSGLTAQL
                     HETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGMT
                     GILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMVAQGNVLPVLYG
                     EMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            631..>2801
                     /locus_tag="ACHJWA_004856"
     CDS             631..>2801
                     /locus_tag="ACHJWA_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO4311273.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQKVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGASFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAILRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAGYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDLSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTP"
BASE COUNT          604 a          738 c          921 g          538 t
ORIGIN      
        1 aaaacggggg ctgaagccat ccgcgcactg gccacacagc tcccggtgtt tcgtcagaag
       61 ctgagcgacg gctggtatca ggtacggatt accgggcggg acgtcagcac gtccgggtta
      121 acggcgcagt tacatgagac tctgcctgat ggcgctgtga ttcatattgt tcccagagtc
      181 gccggggcca agtcaggtgg cgtattccag attgtcctgg gggctgccgc cattgccgga
      241 tcattcttta ccgccggagc cacccttgca gcatgggggg cagccattgg ggccggtggt
      301 atgaccggca tcctgttttc tctcggtgcc agtatggtgc tcggtggtgt ggcgcagatg
      361 ctggcaccga aagccagaac tccccgtaca cagacaacgg ataacggcaa acagaacacc
      421 tatttctcct cactggataa catggttgcc cagggcaatg ttctgccggt tctgtacggt
      481 gaaatgcgcg tggggtcacg cgtggtttct caagagatca gcacggcaga cgaaggggac
      541 ggtggtcagg ttgtggtgat tggtcgctga tgcaaaatgt tttatgtgaa accgcctccg
      601 ggcggtttta tcgtttatgg agcatgacga atgggtaaag gcagcagtaa ggggcatacc
      661 ccgcgcgaag cgaaggacaa cctgaagtcc acgcagctgc tgagtgtgat cgatgccatc
      721 agcgaagggc cggttgaagg tccggtggat ggattaaaaa gcgtgctgct gaacagtacg
      781 ccggtgctgg acagtgaggg gaataccaat atatccggcg tcacggtggt gttccgggcc
      841 ggtgagcagg agcagacacc gccggaggga tttgaatcct ccggctccga gacggtgctc
      901 ggtacagaag tgaaatatga cacgccgatc acccggacca tcacgtcggc aaacattgac
      961 cgtctgcgtt ttactttcgg cgtgcaggca ctggtggaaa ccacctcaaa gggggacagg
     1021 aatccatcgg aagtccgcct gctggttcag atacaacgta acggtggctg ggtgacggaa
     1081 aaagacatca ccattaaggg taaaaccact tcacagtatc tggcctcggt ggtggtggat
     1141 aacctgccgc cgcgcccgtt taatatccgg atgcgcagga tgacgccgga cagcaccaca
     1201 gaccagctgc agaacaaaac gctctggtcg tcatacaccg aaattatcga tgtgaaacag
     1261 tgctacccga acacggcact ggtcggcgtg caggtggact cggagcagtt cggcagccag
     1321 aaggtgagcc gtaattatca tctgcgcggg cgtattctgc aggtgccgtc gaattataac
     1381 ccgcagacgc ggcaatacag cggtatctgg gacggaacgt ttaaaccggc atacagcaac
     1441 aacatggcct ggtgtctgtg ggatatgctg acccatccgc gctacggcat ggggaaacgt
     1501 cttggtgcgg cggatgtgga taaatgggcg ctgtatgtca tcggccagta ctgcgaccag
     1561 tcagtgccgg acggctttgg cggcacggag ccgcgcatca cctgtaatgc ctacctgacc
     1621 acacagcgta aggcgtggga tgttctcagc gatttctgct cggcgatgcg ctgtatgccg
     1681 gtatggaacg ggcagacgct gacgttcgtg caggaccgac cgtcggataa ggtgtggacc
     1741 tataaccgca gtaatgtggt gatgccggat gatggcgcgt cgttccgcta cagcttcagc
     1801 gccctcaagg accgccataa tgccgttgag gtgaactgga ttgacccgga taacggctgg
     1861 gagacggcga cagagcttgt ggaggacacg caggccattc tccgttacgg tcgtaacgtc
     1921 acgaagatgg atgcctttgg ctgtaccagc cgggggcagg cacaccgcgc cgggctgtgg
     1981 ctgattaaaa cggagctgct ggagacgcag accgtggatt tcagcgtggg cgcagaaggg
     2041 cttcgccatg taccgggcga tgtcattgaa atctgtgatg atgactatgc ggggatcagc
     2101 atcggtgggc gtgtgctggc ggtgaacagc cagacccgga cgctgacgct cgaccgtgaa
     2161 atcacgctgc catcctccgg taccacgctg ataagcctgg ttgacggaag tggcaatccg
     2221 gtcagcgtgg aggtccagtc cgtcaccgac ggcgtgaagg taaaagtgag ccgtgttcct
     2281 gacggcgttg ccggatacag cgtatggggg ctgaagttgc cgacgttgcg ccagcgcctg
     2341 ttccgctgcg tgagtatccg tgagaacgac gacggcacgt atgccatcac cgccgtgcag
     2401 catgtacccg aaaaagaagc catcgtggat aacggggcgc actttgacgg cgacctgagc
     2461 ggcacggtga atggcgtcac gccgcccgcg gtgcagcacc tgactgccga agtcaccgca
     2521 gacagcgggg aatatcaggt gctggcgcgc tgggacacgc cgaaggtggt gaagggggtg
     2581 agcttcctgc ttcgcctgac cgtggcagcg gacgatggca gtgagcggct ggtcagtacg
     2641 gccaggacga cggaaaccac ataccgcttc acgcaactgg cgctggggaa ctacaggctg
     2701 actgtccggg cggtaaatgc gtggggacag cagggcgatc cggcatcggt atcgttccgg
     2761 attgccgcac cggcagcgcc gtctcggatt gagctgacac c
//
LOCUS       DAZFAB010000234         2777 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZFAB010000234 DAZFAB010000000
VERSION     DAZFAB010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.235"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..546
                     /locus_tag="ACHJWA_004857"
     CDS             <1..546
                     /locus_tag="ACHJWA_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO4311274.1"
                     /translation="KTGAEAIRALAMQIPAFRQKLSDGWYLVRIAGRDTGENELSARL
                     NEPLANGAVIHIVPRLAGAKSGGVFQAVLGAALIAVAWWNPVGWLGAAAVSGMYAAGA
                     SMILGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMVAQGNVLPVLYGEMRVGSRV
                     VSQEISTADEGDGGQVVVIGR"
     gene            607..>2777
                     /locus_tag="ACHJWA_004858"
     CDS             607..>2777
                     /locus_tag="ACHJWA_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO4311275.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALRETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYDPEKRTYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWTDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEGIVDNGAHFDGDQSSTVNGVTPPAVQHL
                     TAEVSADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSQIELTP"
BASE COUNT          603 a          714 c          933 g          527 t
ORIGIN      
        1 aaaacggggg ccgaagccat ccgggcgctg gccatgcaga tcccggcgtt tcgtcagaag
       61 ctgagcgacg gctggtatct ggtacggatt gccgggcgtg atacaggtga aaatgaatta
      121 tctgcccgtc ttaatgagcc gctggcaaat ggtgccgtga tccacatcgt gccgcgcctg
      181 gcgggtgcta aaagtggcgg tgtgtttcag gcagtgctgg gtgcggcgct gattgcggtg
      241 gcatggtgga accctgtggg ctggctgggg gccgcggctg tatcgggtat gtatgcagca
      301 ggggccagta tgatcctggg tggtgtggcg cagatgctgg caccgaaagc cagaactccc
      361 cgtacacaga caacggataa cggcaaacaa aacacctatt tctcctcact ggataacatg
      421 gttgcccagg gcaatgttct gcccgttctg tacggtgaaa tgcgcgtggg atcacgtgtg
      481 gtttctcagg agatcagcac ggcagacgaa ggggacggtg gtcaggttgt ggtgattggt
      541 cgctgatgca aaatgtttta tgtgaaaccg cctccgggcg gttttgtcgt ttatggagca
      601 tgacgaatgg gtaaaggaag cagtaagggg cataccccgc gcgaagcgaa ggacaacctg
      661 aagtccacgc agctgctgag tgtgatcgat gccatcagcg aaggaccgat tgaaggtccg
      721 gtggatggat taaaaagcgt gctgctgaac agtacgccgg tgctggacag tgaaggtaat
      781 accaacatct ccggtgtcac ggtggtgttc cgggccggtg agcaggagca gacaccgccg
      841 gagggatttg aatcctccgg ttctgagacg gtgctgggta cggaagtgaa atacgacacg
      901 ccgatcaccc gcaccatcac gtcggcaaac atcgatcgtc tgcgctttac tttcggtgtg
      961 caggcactgc gggaaaccac ctcaaagggg gaccggaatc cgtcggaagt ccgcctgctg
     1021 gttcagatac agcgtaatgg tggctgggtg acggaaaaag acatcaccat taagggcaaa
     1081 accacgtcgc agtatctggc ctcggtggtg gtggataacc tgccgccgcg cccgtttaat
     1141 atccggatgc gcaggatgac gccggacagc accacagacc agctgcagaa caaaacgctc
     1201 tggtcgtcat acaccgaaat catcgatgtg aaacagtgct acccgaacac ggcactggtt
     1261 ggcgtgcagg tggactcgga gcagtttggc agtcagcagg tgagccgtaa ttatcatctt
     1321 cgcgggcgca ttctgcaggt gccgtcaaac tatgatccgg aaaaacgcac ttacagcggc
     1381 atctgggacg gaacgttaaa accggcatac agcaacaaca tggcctggtg tctgtgggat
     1441 atgctgaccc acccgcgcta cggcatgggg aaacgtcttg gtgcggcgga tgtggataaa
     1501 tgggcgctgt atgtcatcgg ccagtactgc gaccagtcag taccggacgg ctttggcggc
     1561 acggagccgc gcatcacctg taatgcgtac ctgaccacgc agcgcaaggc gtgggatgtg
     1621 ctcagtgatt tctgctcggc gatgcgctgt atgccggtat ggaacgggca gacgctgacg
     1681 ttcgtgcagg accggccgtc ggataaggtg tggacctata accgcagtaa tgtggtgatg
     1741 ccggatgatg gcgcgccgtt ccgctacagc tttagcgccc tgaaagaccg ccataatgcc
     1801 gttgaggtga actggactga cccggacaac ggctgggaga cggcgacaga gcttgtggag
     1861 gacacgcagg ccattgcccg ttacggtcgt aacgtcacga agatggatgc ctttggctgt
     1921 accagtcggg ggcaggcaca ccgtgccggg ctgtggctga ttaaaacgga actgctggaa
     1981 acgcagaccg tggacttcag cgtgggcgca gaagggcttc gccatgtacc gggcgatgtc
     2041 attgaaatct gtgatgatga ctatgcgggg atcagcatcg gtgggcgtgt gctggcggtg
     2101 aacagccaga cccggacgct gacgctcgac cgtgaaatca cgctgccatc ctccggcacc
     2161 acgctgataa gcctggttga cggaagtggc aatccggtca gcgtggaggt tcagtccgtc
     2221 accgacggcg tgaaggtaaa agtgagccgg gttcctgacg gtgttgctga atacagcgtg
     2281 tgggggctga agctgccgac gctgcgccag cgcctgttcc gctgcgtgag tatccgtgag
     2341 aacgacgacg gcacgtatgc catcactgcc gtgcagcatg tgccggagaa agagggcatc
     2401 gtggataacg gggcgcactt tgacggtgac cagagcagca cggtgaatgg tgtcacgccg
     2461 ccagcggtgc agcacctgac cgccgaagtc tccgcagaca gcggggaata tcaggtgctg
     2521 gcgcgatggg acacgccgaa ggtggtgaag ggtgtgagct tcctgcttcg cctgaccgtg
     2581 gcagcggatg acggcagtga gcggctggtc agcacggccc ggacgacgga aaccacatac
     2641 cgcttcacgc agctggcgct ggggaactac aggctgacag tccgggcggt aaatgcgtgg
     2701 ggacagcagg gcgatccggc gtcggtatcg ttccggattg ccgcaccggc agcgccgtca
     2761 cagattgagc tgacacc
//
LOCUS       DAZFAB010000235         2757 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZFAB010000235 DAZFAB010000000
VERSION     DAZFAB010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.236"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(132..401)
                     /locus_tag="ACHJWA_004859"
     CDS             complement(132..401)
                     /locus_tag="ACHJWA_004859"
                     /inference="COORDINATES: protein motif:HMM:NF040040.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6258 family protein"
                     /protein_id="HFO4311276.1"
                     /translation="MEFDLWSKQIRIQVNLISRIAYGTTEWNFYDNEDLEDGYLVFSG
                     VSFFNITPNGSIPNDYIISIITDNVNVEYFESTIVSVGQIPNTNS"
     gene            complement(988..1188)
                     /locus_tag="ACHJWA_004860"
     CDS             complement(988..1188)
                     /locus_tag="ACHJWA_004860"
                     /inference="COORDINATES: protein motif:HMM:NF026967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTF2 fold immunity protein"
                     /protein_id="HFO4311277.1"
                     /translation="MALELSYVYIKYVYGKEKAEFQKPYSITDDNNCWKIEGKQPKNS
                     GGNFTMLIAKKDGQVLNVIHTK"
     gene            complement(1459..2001)
                     /gene="ybfC"
                     /locus_tag="ACHJWA_004861"
     CDS             complement(1459..2001)
                     /gene="ybfC"
                     /locus_tag="ACHJWA_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfC"
                     /protein_id="HFO4311278.1"
                     /translation="MIFVSFGVIADCEIQAKDHDCFTIFAKGTIFSAFPVLNNKAMWR
                     WYQNEDIGEYYWQTELGTCKNNKFTPSGARLLIRVGSLRLNENHAIKGTLQELINTAE
                     KTAFLGDRFRSYIRAGIYQKKSSDPAQLLAVLDNSIMVKYFKDEKPTYARMTAHLPNK
                     DESYECLTKIQHELLRSEEK"
     gene            complement(2026..>2757)
                     /locus_tag="ACHJWA_004862"
     CDS             complement(2026..>2757)
                     /locus_tag="ACHJWA_004862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO4311279.1"
                     /translation="LISKEGATEWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEES
                     GLYYNRHRYYDPLQGRYITQDPIGLKGGWNFYQYPLNPVQYIDSMGLASKYGHLNNGG
                     YGARPNKPPTPDPSKFPDIAKQLRLPYPIDQASSAPNVFKTFFRALSPYDYTLYCRKW
                     VKPNLTCTPQDDPQYPGMDTKTASDYLPQTNWPTTQLPPGYTCAEPYLFPDINKPDGP
                     ATAGIDDLGEILAKMKQRTSRGIRK"
BASE COUNT          766 a          577 c          469 g          945 t
ORIGIN      
        1 taatgatctt tccctgtcta aaatgcctat acataacatg gttattcaat tattttttct
       61 ttgaatttat tctcaatcca gctgtgttta cacctgatga aaatttgaca ctcaccaata
      121 ttatcaatgt ctcaagaatt tgtatttgga atttgtccaa ctgaaactat agttgattca
      181 aaatattcaa cattgacatt atctgtaatt attgaaatta tataatcatt tggaatagag
      241 ccattaggag ttatattgaa gaaactaaca cctgaaaata ccaaataacc atcttctaag
      301 tcttcattat cataaaaatt ccattctgtt gtaccatagg ctatccttga gattaaatta
      361 acctgaattc taatttgttt actccataaa tcaaattcaa tttttttatt gctcgatcac
      421 caagatatat tctatcaaac atattacccc cctattctaa aaatactcag gcattccgca
      481 cattatatca gcctcatctt gattgcttcc cgtttcccga tacggcggcg cggggagtta
      541 taaaggtaag aaatttccag cagtacggcg tcctattgtt cacggcggta atgcaccagc
      601 cagtattggc tatcgtagtg ataaccgtgt aacctgccgc cgcatcgcgt atctaatgtt
      661 cattcttttc cataaaccgt ccgtacacat tgtgatagta aaaatacatc ttcgctgatg
      721 cggttattct gctgcatgtg gggagcgccg aatactgctc ccggtatggc cagtaattac
      781 tatttagtac gacgttaggg ggagtcactt aatctcacag atacttcaag taacagatga
      841 aaatcgctcc tgaaatgaaa aaagaaattt tttgaatgta ttcctgtcaa atcaagtggt
      901 tttttcgtcg atatgtagag tcatattgta cacaaaattg ttacgaaaca caccttggta
      961 aaatatttga atcgactgta cagtggatta ttttgtgtgt attacattca acacttgccc
     1021 atcttttttc gctattagca ttgtaaaatt tcctcctgaa ttcttcggtt gttttccctc
     1081 gattttccaa caattattgt catctgtaat actatatggt ttctgaaatt ctgctttttc
     1141 tttcccgtat acatatttaa tgtaaacata tgataattcc aaagccattt cacgactatt
     1201 tacttaaatt tccactggat tttcttttcc aaatactgag tgtgatattg taaacaatat
     1261 cataataagt aatattagtg agtttctcat gttctacacc tatcgaatct caaaagttgt
     1321 gggtaaaaca ttacattcag aatatatgat attcttacca tcggaatcca tttttccaaa
     1381 tcgaccttct ggtgtacctg gatatccatt aagaacatgt ttttccgaat ggttaattta
     1441 ttttctcagg aagatgtttt atttttcttc actacgaagc agttcatgct gtatttttgt
     1501 taaacattcg taagactcat ctttgttcgg gagatgtgct gtcattcgag cataagttgg
     1561 cttctcatct ttaaaatatt taaccatgat tgaattatca aggactgcca acaactgagc
     1621 tggatcactg ctcttttttt gatagatacc cgctcttata taacttctaa accgatcgcc
     1681 gagaaacgcc gttttttctg ctgtatttat caactcctgc aacgttcctt tgatggcatg
     1741 attttcatta aggcgtagtg acccgacacg aattaagagt ctcgccccgc ttggtgtgaa
     1801 tttattattt ttacatgtac ccagttctgt ttgccaataa tactcaccga tgtcctcatt
     1861 ctgataccat ctccacatgg ctttattatt taagacagga aacgcggaaa aaatagtacc
     1921 cttagcgaaa atagtaaaac aatcatgatc tttagcttgt atttcgcaat cagctataac
     1981 accaaaacta acaaatatca tcagcaaaaa aaaacaaaac tcttttcatt ttcttattcc
     2041 tctcgatgtt ctctgtttca ttttagctaa aatttcaccc aaatcatcta tccctgctgt
     2101 tgctggccca tcgggtttat taatgtcagg gaataaatag ggttctgcac aagtatatcc
     2161 tggtggtaat tgagttgttg gccaatttgt ctgtggcagg taatcacttg ctgtctttgt
     2221 atccatccct ggatactggg gatcatcctg tggcgtacaa gtcagatttg gttttaccca
     2281 cttcctgcaa tacagtgtgt agtcgtaagg gcttaatgct ctgaagaatg ttttgaaaac
     2341 attaggcgca ctactggcct ggtcaatagg atatggcagt cttaattgtt tcgctatgtc
     2401 tggaaattta cttggatcgg gcgtaggcgg tttgttgggt ctcgctccat atccgccatt
     2461 atttaagtgt ccatattttg atgccagtcc cattgaatct atatactgaa ccggattcaa
     2521 cggatactga taaaaattcc atcccccctt cagtccaatc ggatcctgag tgatatacct
     2581 cccctgcagc gggtcataat agcggtggcg gttgtaatac aggccggact cctcatcata
     2641 ctgctgcccc ggcaggcgga taagctgctg cagctgatgc gggttctctt cattcagcag
     2701 gttgccccac tcatcgtatt ctgcgcacca ttctgttgcc ccttccttac tgataag
//
LOCUS       DAZFAB010000236         2696 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZFAB010000236 DAZFAB010000000
VERSION     DAZFAB010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.238"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..549
                     /locus_tag="ACHJWA_004863"
     CDS             <1..549
                     /locus_tag="ACHJWA_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFO4311280.1"
                     /translation="LVHSHPGGLPWLSEADRRLQIKSALSWWLVCRGEIHKFRCVPHL
                     TGRRFEHGVTDCYTLFRDAYHLAGIDMPDFEREDDWWRNGQNLYLDNMAVTGFYRVPL
                     SSAQAGDILLCCFGASVANHAAIYCGNGELLHHLPEQLSKRERYSEKWQRRTHSAWRH
                     RHWHVSAFTGIYNDLAAASACM"
     gene            546..1127
                     /locus_tag="ACHJWA_004864"
     CDS             546..1127
                     /locus_tag="ACHJWA_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO4311281.1"
                     /translation="MNTAAEAIRALSLQVPGFRRQMNEGWYQIRIRGEDTAPEAVYAR
                     LHEPLGEGAVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMALWGSALAAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKAKTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGDGKVVVIGRQA"
     gene            complement(1318..1845)
                     /gene="sodC"
                     /locus_tag="ACHJWA_004865"
     CDS             complement(1318..1845)
                     /gene="sodC"
                     /locus_tag="ACHJWA_004865"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFO4311282.1"
                     /translation="MKCKIIAAIAMLTAASCGYAAEQEVPMNLVSADGKEVSIGKITI
                     QETPYGLLFTPALHSLSEGIHGFHVHEKGNCAPALKDGKPVAALSAGGHFDPKNTGKH
                     LGPWSPDGHLGDLPALFVTHDGKANYPVLAPRLNSLKEIKGRSLMLHAGGDNHHDHPE
                     PLGGGGARMACGIIQ"
     gene            1979..>2696
                     /locus_tag="ACHJWA_004866"
     CDS             1979..>2696
                     /locus_tag="ACHJWA_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4311283.1"
                     /translation="MGKGGGRAHTPVEAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETALGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLLETTSKGDRNPSSVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQFLASVILDNLPPRPFNIRMVRETADSTSDQLQNKTLWSSYTEIIDVKQCYPNTAIV
                     GLQVDAEQFGGQQMTVNYHIR"
BASE COUNT          603 a          688 c          816 g          589 t
ORIGIN      
        1 ctggtccaca gccaccccgg tggtctgccc tggctgagcg aggcggaccg gcggctgcag
       61 ataaaaagtg cactgtcctg gtggctggtc tgccgggggg aaattcataa attccgctgt
      121 gtgccacatc tgacaggacg gcgctttgag cacggggtga cggactgtta cacgctgttc
      181 cgggatgcct accatctggc gggaattgat atgccggatt ttgagcgtga ggatgactgg
      241 tggcgtaacg gtcagaacct ttacctggac aatatggcgg tcaccggctt ttaccgggtg
      301 cccctgtcct ctgcacaggc gggcgatatc ctgctgtgct gctttggcgc atcggtggcc
      361 aatcatgccg ccatttactg cggcaacggt gaactgcttc accatctgcc tgaacaactg
      421 agtaaacggg agaggtattc cgaaaaatgg caacgacgaa cgcattcagc ctggcgtcac
      481 cgccactggc acgtatctgc cttcacgggg atttacaacg atttagccgc cgcctcagcc
      541 tgtatgtgaa cacggcagcg gaagccatcc gggcgctgtc gttacaggtg ccgggattcc
      601 gccgtcagat gaacgaaggc tggtaccaga tacgtattcg cggtgaggac acggcaccgg
      661 aggcggtgta cgcccgtctt cacgaacctc tgggtgaggg ggcggtcatc catattgtgc
      721 cgcgactggc cggagccgga aagggcggac tgcagattgt gctgggggcg gcggccatcg
      781 tggggtcgtt cttcactgcc ggggcatcaa tggcgttatg gggttcagcc ctggcagccg
      841 gtggtttttc tgccaccacg atgctgtttt cacttggagc cagcatgatt ctgggcggtg
      901 tggcccagat gctggccccg aaggcaaaaa caccggatta ccgcgcaacg gataacggca
      961 gacagaacac gtacttttcc tcgctggata acatgattgc ccaggggaac ccgatgccgg
     1021 tgccttacgg tgaaatgctg gttggctccc ggcgaatctc ccaggacatc agtacccgtg
     1081 atgaaggcgg tgacgggaag gtggtggtta tcgggcggca ggcataaaag cgaaaaaatc
     1141 ccgcagtgct cacggacagg aactgcggga gcgttacgaa gattgagtgt aaggaattat
     1201 tcttatgtca cgacaaaaac attaactcag agagggagga tgtgccgttc ttttcaggga
     1261 gaaaggattt atcgtcctga ggaataaagg taaggggccc gccccttacc tgactgatta
     1321 ttgaatgatg ccgcaggcca ttctcgcacc accaccgccc aggggctccg gatggtcatg
     1381 atggttatca ccgccagcat gaagcatgag agaacgccct ttaatctctt ttaatgagtt
     1441 cagtctcggg gccaggaccg ggtagttcgc ttttccgtca tgcgtcacga acagcgcagg
     1501 gaggtcgccc aggtgtccat ccggagacca ggggccaaga tgtttgccgg tgtttttcgg
     1561 gtcaaagtga ccgccagccg ataatgctgc gaccggtttt ccgtctttca gtgccggggc
     1621 gcaatttcct ttttcgtgca catgaaaacc atgaatgcct tcagacagag agtgaagggc
     1681 tggtgtgaac agcagaccgt agggggtctc ctgaatggtt atttttccaa tgctgacttc
     1741 ttttccgtca gcactgacaa ggttcattgg gacttcctgt tctgctgcgt atccgcatga
     1801 tgctgctgtc agcatggcaa tggcagcaat gattttacat ttcataaaac cctcattaat
     1861 tccgttaaca gactgagctt gctggttaca gggtaacaaa cagcgttctg atgatatcgc
     1921 gcaatagctg tgcaatatcc tatcactgcg attaataata ccaattgaga ggaacattat
     1981 gggtaaaggt ggcggcaggg cgcacacgcc ggttgaggca aaggacaatc ttaagtccac
     2041 gcagatgatg agcgtgattg atgccattgg tgaagggccg attgaaggtc cggtgaaggg
     2101 gctgcagagt atcctggtga acaaaacccc gctgacggac acggacggta atcctgtgat
     2161 acatggtgtg acagcggtct ggcgcgccgg ggagcaggag cagacaccac ctgaaggctt
     2221 tgagtcttcc ggagctgaaa ccgcactggg cgtggaagtg acgaaggcaa agccggtgac
     2281 gcgcaccatt acatccgcga acattgaccg cctgcgggtc accttcgggg tgcagtcact
     2341 gttggagacc acctcaaagg gcgaccgtaa tccctcttct gtccgactgc tgattcagtt
     2401 gcagcgtaac ggtaactggg tgacggaaaa ggatgtcacc attaacggca agaccacctc
     2461 gcagtttctg gcgtcggtga ttctggataa tctgcctccc cgtcctttta acatccggat
     2521 ggtccgggag acggcggaca gcacctcgga ccagctgcag aataagacgc tctggtcgtc
     2581 atacaccgaa atcatcgatg tgaaacagtg ctacccgaac acggcgattg tggggctgca
     2641 ggtggatgcg gagcagtttg gtggccagca gatgacggtg aactaccata tccgag
//
LOCUS       DAZFAB010000237         2642 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZFAB010000237 DAZFAB010000000
VERSION     DAZFAB010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.239"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            3..221
                     /locus_tag="ACHJWA_004867"
     CDS             3..221
                     /locus_tag="ACHJWA_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFO4311284.1"
                     /translation="MKHYLEKNYPRKSRTTEFLFFILFIVLMIPISPLLLVWIIGRTF
                     EPVIELYTDVTWESFSALHNKINPYKEN"
     gene            223..780
                     /locus_tag="ACHJWA_004868"
     CDS             223..780
                     /locus_tag="ACHJWA_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO4311285.1"
                     /translation="MSTITRERAEIKSYITGFLSDSAHDNKSSDSLLANVFRIALASL
                     EAEPIAMVVPDEMDLLTCHLDGVTKTYADGWNACRVAMLQAGNFRENKNSSTNNFREI
                     SETSTRSPITLDGWISCTERMPEKSQNVLISMNIDSEAGPLIYSARYLGGTFRRGGIA
                     VSPGNDLRQATHWMSLPEPPQEVNQ"
     gene            1071..1226
                     /locus_tag="ACHJWA_004869"
     CDS             1071..1226
                     /locus_tag="ACHJWA_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO4311286.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESEK"
     gene            1346..1690
                     /locus_tag="ACHJWA_004870"
     CDS             1346..1690
                     /locus_tag="ACHJWA_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiW/YdeI family stress tolerance OB fold
                     protein"
                     /protein_id="HFO4311287.1"
                     /translation="MKKVLIAALISGVSFGAFAQQGGFQGPEAERSTVAQAKELKDDA
                     WVILEGSIVKKVGDERYEFRDNSGTIVTDIDDSVWAGQNVSPKDKVRIEGEIDKDLSS
                     VEVDVKALKLLK"
     gene            1812..2084
                     /locus_tag="ACHJWA_004871"
     CDS             1812..2084
                     /locus_tag="ACHJWA_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311288.1"
                     /translation="MTQHIKSHNSEADPEIKQGRRFRAPQYGWFHYLFCTIDEADMLQ
                     EAYLRRGVRVERSLNADRLTWTVSVYLPVRAHLPRTHACYRQRVWR"
     gene            2086..>2642
                     /locus_tag="ACHJWA_004872"
     CDS             2086..>2642
                     /locus_tag="ACHJWA_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO4311289.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESLPVFHRGRVLVEPEPKNMRA
                     LPSGAVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHSYGAGAMVLCWHCDNQLRDQTSESLEQLTQQNLTAWMIDVIRHVMNGTQERE
                     LSLAELSWWAVCNQVVDALPEAVSRR"
BASE COUNT          688 a          599 c          716 g          639 t
ORIGIN      
        1 aaatgaaaca ttatttagaa aaaaattacc cacgaaagag cagaacaaca gagtttctgt
       61 ttttcattct gtttatagtg ttgatgatac cgatatcccc gctattactg gtctggataa
      121 ttggaaggac atttgaacca gttattgagc tatataccga tgtgacatgg gaatcattca
      181 gcgcactgca caataaaatt aatccgtata aggaaaactg atatgagcac tattaccaga
      241 gaacgcgcgg agattaaatc atacatcaca ggcttcctga gcgactcggc gcacgataac
      301 aagtcttcag acagcctgct ggctaatgtg tttcgtatcg cgctggcatc actggaagca
      361 gagccgatag caatggtagt gcctgatgaa atggatttgc ttacctgcca tctcgacggt
      421 gtaactaaaa catatgctga tggctggaac gcctgccgcg tcgccatgct tcaggccgga
      481 aactttcggg aaaataagaa ttcgtcaacc aacaattttc gggaaatctc ggaaacgtca
      541 accagatctc cgataactct ggatggctgg ataagctgta ctgagcgaat gcctgaaaag
      601 agccagaacg tgcttatttc gatgaatatc gatagcgagg ctgggccatt aatatattcc
      661 gcacgctatc tcggaggcac gttccggcgc ggaggtatag cagttagtcc gggtaatgat
      721 cttaggcaag caacccactg gatgtcgcta ccagaaccgc cgcaggaggt gaatcaatga
      781 cctggcctga agcattcaca acggtaggaa ttgcgatggc ggtggcgctg gtggtgtatt
      841 cgatttgccg ctggggataa aaacggtttg cgggaaaagg atagttaagt agaattgctg
      901 cgggtgcttg aggctatctg cctcgggcat gaacaccaac ggcagataga gaaaagcccc
      961 agttaacatt acgcgtcctg caagacgctt aacattaatc tgaggctcaa tccatgctga
     1021 acacatgtag gttagcctct tacgtgccga aaggcaagga gaagcaggct atgaagcagc
     1081 aaaaggcgat gttaatcgcc ctgatcgtca tctgtttaac cgtcatagtg acggcactgg
     1141 taacgaggaa agacctctgc gaggtgcgaa tccgaaccgg tcagacggag gtcgctgtct
     1201 tcgtagacta cgaatctgag aagtaagaga ccaggcgggg gagtaatctc ccgccacctc
     1261 tgatgtgtca ggcatcctca acgcacccgc gctttaccat actgaaaatg ctgtttgaat
     1321 gttcatctct gaaagaggac tatgaatgaa aaaggtattg attgcagcac ttatttccgg
     1381 tgtgtctttt ggcgcttttg cacagcaggg tggtttccag gggccagaag cagagcgttc
     1441 aacagtagcg caggcaaaag aactgaagga tgatgcatgg gttatccttg aagggagcat
     1501 cgttaaaaaa gtgggtgatg aacgttatga gtttcgtgac aatagcggga caattgtcac
     1561 ggatattgat gacagcgtat gggccgggca gaatgtttct ccgaaagaca aagtaagaat
     1621 tgagggtgaa attgataaag acctgagcag tgttgaagtt gatgtaaagg cactgaaatt
     1681 attaaagtaa ccgcccctgc ttgttaagcc cgtcttactg acgggttttc tgtttgtaca
     1741 ttccggcgta ttgccttaca attcgcgcag tcagcctgaa caactgacac ctgctgtcac
     1801 cggagaatcc gatgacacaa cacataaaat cccacaattc tgaagccgac ccggaaatta
     1861 agcaggggag gcgttttcgt gcgcctcagt atggctggtt tcactatctg ttctgtacga
     1921 tcgatgaggc agatatgctt caagaggcgt atctgcgtcg cggtgtccgt gtggagcgga
     1981 gtctgaacgc tgatcgtctg acctggaccg tttctgtata tcttcctgtt cgtgcacatc
     2041 tgccacggac acatgcctgc taccgtcagc gcgtctggag gtaatgtgcg ggtattactt
     2101 cgacctgttc tggttccgga actcgggctg gtggtcctta agcccggtcg tgaatcattg
     2161 ccagtttttc atcgcggcag ggtgctggtg gagccggaac cgaaaaacat gcgggcgctg
     2221 ccatctggag cggttcctgc tgttcgccag ccgctggcgg aagataaatc actgctgcca
     2281 tttttcagcg atgagcgggt gattcgtgca gctggcggcg ctggtgcact gtctgactgg
     2341 ttattacgtc acgtgaaatc ctgccagtgg ccacacggcg attatcatca cagcgaaacc
     2401 gttattcaca gttacggtgc tggcgcaatg gtgttgtgct ggcactgcga caaccagctg
     2461 cgcgaccaga cctccgaatc acttgagcaa cttactcaac aaaatctgac agcctggatg
     2521 attgacgtca tacgccatgt aatgaatggc acgcaggagc gggaattatc gctggctgaa
     2581 ttatcctggt gggcagtctg caatcaggtg gtggacgcat tacctgaggc agtatcgcgt
     2641 cg
//
LOCUS       DAZFAB010000238         2637 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZFAB010000238 DAZFAB010000000
VERSION     DAZFAB010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.240"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..1661)
                     /locus_tag="ACHJWA_004873"
     CDS             complement(<1..1661)
                     /locus_tag="ACHJWA_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO4311290.1"
                     /translation="MNISNSQIDILRRDVRAGLRALFRPEPQTAVEWADASYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLSLAPWYGKKHRDNTLTMKRFTNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEYYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDL"
     gene            complement(1636..2181)
                     /locus_tag="ACHJWA_004874"
     CDS             complement(1636..2181)
                     /locus_tag="ACHJWA_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO4311291.1"
                     /translation="MKVNKKKLAEIFNVDPRTIERWQSQGLPCVSGGGKGVESVFDTA
                     TAIQWYAQREADIENEKLRKEVEDYRAASEADLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSD"
     gene            2568..>2637
                     /locus_tag="ACHJWA_004875"
     CDS             2568..>2637
                     /locus_tag="ACHJWA_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311292.1"
                     /translation="MSSKNRTRRTTTRNIRFPNQMIE"
BASE COUNT          647 a          738 c          615 g          637 t
ORIGIN      
        1 agatcaaaaa tatccgggtt attcgggaag tgaacggcac cgggaagcgg ttcatcccct
       61 tccggcgtca gtgtgaagcg gttataaatc tgctctttcg cggtatccgt accgatttcg
      121 gtaaggtaaa ccccgttttt gtttcgctta cgtggcatgc tggccaccgg ctttccgtag
      181 acggatgccc ctttaatggg gatcacccgg aacagcccat gttttttcga gcgttcatac
      241 acaatggtcg ggtcaatccc gccagtatcc cagcagatac gggataccga catttctgca
      301 ccattccggc gggtataggt tttattgatg gcctcatcca cacgcagcag cgtctgttca
      361 tcgtcgtggc ggcccataat aatctgccgg tcaatcagcc agctttcctc acccggcccc
      421 catccccata cgcgcatttc gtagcggtcc agctgggagt cgataccggc ggtcaggtaa
      481 gccacacgat caggaacggg cgctgaataa tactctttcc gctctgccat cacttcagca
      541 tccggacgtt cgccgatttt cgcttcccac gtctcaccga gcgtggtgtt cacgaaggtt
      601 ttacgttttc ccgtatcccc tttcgtcttc atccagtctt tgacaatctg cacccaggtg
      661 gtgaacgggc tgtacgccgt ccagatgtga aaggtcacac tgtcaggcgg ttcaatctct
      721 tcaccggatg acgaaaacca gagaatgcca tcacgggtcc agatcccggt cttttcgcag
      781 atataacggg catcagtaaa gtccagctcc tgctggcgga tgacgcaggc attatgctcg
      841 cagagataaa acacgctgga ggggtcatcc ggcgtccatt tgaggccaaa cggcgtctct
      901 ttgtcgccaa atttaagata ctgctcctcc ccgcagtgcg ggcaggcaac atgaaaacgc
      961 ataaaatgcg gggattcact ggctgcacgc tcaatctggc aggtgcctct cactttgggc
     1021 gtggagccac ggatggactt tggccagacc gagccttcaa tacgcttgtc acccaggaac
     1081 gtcggagagc cttcctgttc aatatcctca tcaaaggcag caagttcatc ataacccgcc
     1141 acatcaaccg acttttcacg gtagtttttt gccgctttac cgcccaggca ccagaagcca
     1201 cgaccattgg tgaaacgctt catagtgagc gtgttatccc ggtgcttttt gccataccac
     1261 ggggccagag acagcagcga cggaatatcg cggatggtag gctcgacgtg ggttttcata
     1321 aagttctcgg catcaccatc cgtcggcaac cagataaggg tgttacgctg cttatgctct
     1381 atgaagtagg cataaacacc cagcagcatt tttgaataac caacacgggc agacttcacc
     1441 acattcacct cgcggatgta gtcgctgccc atcgcattca tgatagcccg ctgaaagggt
     1501 agtgtttccc agcgcccttc ctggtatgcg gattctttcg ggagatagta actggcatcc
     1561 gcccattcaa cggcagtctg tggctccggc ctgaacaggg ctcgcagccc ggcgcgtaca
     1621 tcacgccgca gaatatcaat ctgactgttc gatatattca ctcagcaacc ccggtatcag
     1681 ttcatccagc gcggctgctt tgttcatggc tttgataata tcccgtttca ggaaatcaac
     1741 atgtcggttt tccagttccg gaaaacgccg ctgtactgag agagggatcc cgtcaagaat
     1801 actggcaatt tcacctgcga tccgtgacag cacgaaagta cagaatgcgg tttccaccac
     1861 ttctgcggag tctctggcat tcttcagctc ctgggcgtca gcctgcgcac gcgtaagtcg
     1921 atggcgttcg tactcaatag tcccaggctg gagatctgcc tcgctggcag ccctgtaatc
     1981 ctcaacctct ttacggagtt tttcattttc gatatcagct tccctctgcg cataccactg
     2041 aattgccgtg gcggtatcaa atacagattc aacgccctta ccacctccgg agacgcaagg
     2101 gagcccctga gactgccagc gttcaatcgt tcgcggatcc acgttgaaaa tttcggcaag
     2161 tttcttttta ttaaccttca tgaaacagtc tcacaacaaa tacagggtcc gacatgaaag
     2221 tgcccgaaaa tgactttttc aggcgttttc atgtcggacc tttacggatt cgatattaga
     2281 aaaaacaaat agttatgttc gagaagtacc gacatgattt tccccggaaa attttcataa
     2341 atagcgaaaa cccgcgaggt cgccgccccg taaccggtcg gatcgccgga aaggacccgc
     2401 aaaatgataa taattatcat ctacatgtca caacgtgcat ctacgccatc aaaccacgtc
     2461 aaataatcaa ttatgacgca ggtatcgtat taattgatct gcatcaaatt aacgtaaaag
     2521 caacttcaga taatacaaat cagcaacact gaatatgggg caacattatg tcatcaaaga
     2581 acagaacccg cagaacaaca acccgcaaca tccgatttcc aaaccagatg attgaac
//
LOCUS       DAZFAB010000239         2612 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZFAB010000239 DAZFAB010000000
VERSION     DAZFAB010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.241"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(286..543)
                     /locus_tag="ACHJWA_004876"
     CDS             complement(286..543)
                     /locus_tag="ACHJWA_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311293.1"
                     /translation="MSDRVIECASRAGRDFSEFMKGEKGMMEALASVDEFGEQLRLNG
                     CVNHHFVSYMMRNSIMQAFMDMAKAERKEERRRKRAEAKAK"
     gene            complement(540..1037)
                     /locus_tag="ACHJWA_004877"
     CDS             complement(540..1037)
                     /locus_tag="ACHJWA_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFO4311294.1"
                     /translation="MKSLTLFNQPIRIGEDGMICLTDMWKASGKSESESPYHYLRNKQ
                     TKEFLAELEKNHESVVFTERGAHGGTYGGKFVAYDYAAWLNPGFKYAAYKVLDDYFTG
                     ELHHRNSLSAQLNMKCHEFDQKKDMASFCGQGLAAWRYTKPGLIAEINSLANQLQISI
                     PGLPG"
     gene            complement(1240..1677)
                     /locus_tag="ACHJWA_004878"
     CDS             complement(1240..1677)
                     /locus_tag="ACHJWA_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO4311295.1"
                     /translation="MSRVTAIIYVLVICLIVCLSWAVNHYRDNAIAYKEQRDKATSII
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVSATCAKSTTGASSM
                     GDGESPGLTADAELNYYRLRGGIDKITAQVNYLQEYIRTQCLK"
     gene            complement(1674..2171)
                     /gene="rrrD"
                     /locus_tag="ACHJWA_004879"
     CDS             complement(1674..2171)
                     /gene="rrrD"
                     /locus_tag="ACHJWA_004879"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFO4311296.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMPGKTYTEAECKALLNKDLATVARQINPYIKIDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            complement(2171..2386)
                     /locus_tag="ACHJWA_004880"
     CDS             complement(2171..2386)
                     /locus_tag="ACHJWA_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO4311297.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            complement(2463..>2612)
                     /locus_tag="ACHJWA_004881"
     CDS             complement(2463..>2612)
                     /locus_tag="ACHJWA_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311298.1"
                     /translation="VDAILDELLGVQAEPPTEAGDTTAESGEVQPESPVADATEPQPE
                     SVMML"
BASE COUNT          596 a          701 c          618 g          697 t
ORIGIN      
        1 gtttcttcca ccatcgcacc ggacgggcga ccatgagggg agaacgccgc gctccgttta
       61 cgcggtaaac cccggtgtgt atcgtttttg attatccccg cacactcgcg cagaaggaat
      121 tccccgtcgg gctacggtca tggttaatgc ggaaatacag cgacgataca gcgcatgatg
      181 tgtcaggctt gaataccttt atccgttaaa agggatatca gttaagttat cccgtgtagg
      241 gtataagcca ttatcaagcc caccagtaga tgggctttgt aatggctact tcgcttttgc
      301 ttccgctcgc ttacgccggc gctcttcttt cctctcggct tttgccatgt ccatgaatgc
      361 ctgcatgatc gagttccgca tcatgtagct aacaaagtga tgattgacac agccgttgag
      421 gcgcagctgc tcgccaaact catccaccga ggccaatgct tccatcatac ccttctcgcc
      481 tttcatgaac tctgagaagt cgcgccccgc tctggaggcg cattcaatga cacgatcact
      541 catcccggaa gcccggggat cgaaatctgc aactggttag ccagggagtt aatttcagcg
      601 atcaaaccag gtttcgtgta gcgccatgcc gcgaggcctt gtccacagaa gctcgccatg
      661 tcttttttct gatcaaactc atgacatttc atgttgagct gcgcacttaa gctgttccga
      721 tgatgaagct ctccggtgaa gtaatcatcg aggactttat aggctgcata tttaaatccg
      781 gggtttagcc atgctgcgta atcataagca acgaactttc cgccataagt tccaccgtgc
      841 gcaccgcgtt ccgtaaaaac cacagattcg tggtttttct ccagctcagc caagaactct
      901 ttggtctgct tgtttcgcag gtagtggtac ggagattcag attcactttt accactggct
      961 ttccacatat cagtgaggca gatcatgcca tcttcaccga tacggattgg ttgattgaag
     1021 agggttaatg atttcatagc gtgtacctac tctttgaaat gaacctttgc cgcacaggaa
     1081 accagcccac cgaggctcgc cagcactaac tggtatcctc aaaggcccat tccaaagggg
     1141 caggttcggt gtaaaaaaca tgcgttgcgg tacgcattta ttgcaaaaag ccccgcatcg
     1201 cgaggctcat taaatggact ttgtgatttg caaaaaaaat tatttcaggc attgcgtcct
     1261 gatgtactcc tgcaggtagt taacctgcgc ggttatcttg tcgattccac ctcggagacg
     1321 gtaataattg agttcagcat ctgctgtaag tcctgggctt tctccatcgc ccatgctgct
     1381 ggctccggtc gttgactttg cacaggtggc ggagacttgc aggcgcttac gcccagcaga
     1441 aacatcagca cggagacttt cgatagtcgc attagcatca gcaagctcct ttgtgtatct
     1501 ggcgtcaagt tctgctacat cacgttgccg cttctgcata tcagcgatga tggatgtggc
     1561 tttatcgcgc tgctctttgt aggcgatggc gttatcacgg taatgattaa cagcccatga
     1621 aaggcagacg atgaggcaga tgaccagaac gtagataatc gcggttactc tgctcattgt
     1681 tgcccccaca aacagacttc acgctcaatc tcacggcgag tcatcagccc tttccattgc
     1741 ttaccgccag cgtatgtcca gcgccgtagc tgatcacatg cgcctttgat atcgccctgg
     1801 tttattttgc gaagaagcgt cgatgttctg aaattgccag cgcccacgtt gtaaacgaac
     1861 gagtaaagag cgccgcgcgt tgtttccggt atatcgattt tgatgtacgg gttaatttgt
     1921 ctggcgaccg tggcaaggtc tttattcagg agggctttgc attctgcttc ggtatacgtt
     1981 ttaccgggca tgatgtcttt tccggtgtgt ccgtgacata cagtccatac accaacaata
     2041 tctttatatg gtatgtagct gacaccttcc agaccatcgt taccacttgg gccagtaatt
     2101 aacactgatg ctatagcaat tgctccgcca ccaatagcag cagcaacggc ttttcgtaat
     2161 gatggaggca ttattcacct ctcgcagcct tgcgcttatc ttctttaatc ttgaaataaa
     2221 ggtttgtcag gtacgtcagc aggccaaaca gcagactccc cagcacacct attgccaccc
     2281 actgggacgg agagactttg tccagcagct gcagtaacca gtatcccgtc cccaccgctg
     2341 acgtggtgta tgacacacct gttgtgattt tttccatctg gtacataccc cgtctcccgt
     2401 tatccggaag ctcacaacat gaaaaaggcc agcagctgtt tactgatggc cctgactccc
     2461 cgttacagca tcatgaccga ttcgggttga ggttcagtcg catcggcgac cggtgattca
     2521 ggctgaactt caccgctctc tgcggtggta tctcccgctt cagtcggtgg ctctgcctgt
     2581 acaccaagca gctcatccag aatggcatca ac
//
LOCUS       DAZFAB010000240         2544 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZFAB010000240 DAZFAB010000000
VERSION     DAZFAB010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.242"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(66..2174)
                     /locus_tag="ACHJWA_004882"
     CDS             complement(66..2174)
                     /locus_tag="ACHJWA_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFO4311299.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILATILKENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSIDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     RGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKNYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAETIGNNQMIAVTNNQIQTVGVNQIETVGSNQIINVGSVQVETIGLVRA
                     LTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRVGLGYDVKVGNNVTFTVGKTKKDD
                     TGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINWNCAA
                     SKSPPKTPDEKQDTPDMREY"
BASE COUNT          572 a          708 c          618 g          646 t
ORIGIN      
        1 attcaattcc ttcttctgtt tatttttaat gcttgtttgt tactgaatac cgaacaggta
       61 cgggatcagt actctctcat atccggcgta tcctgctttt catcaggggt cttcggtggg
      121 gatttcgagg ctgcacagtt ccagttaatc aacagtgagt caccattaac ctgctcctta
      181 ccctgcaaat gaatttttgt gccgttgaga aaaatttgtc cgtccttcgt cagcaccagc
      241 cttgccttac cacagcagag ctccagatgc tcaccggcgg agtaaatcgc ggtctgcccg
      301 gtatcatcct ttttcgtttt accaacggtg aaggtaacgt tatttccgac tttgacgtca
      361 taacccagcc cgaccctcaa cgatttatgc aaaccgatct gcgaggactg catcagagcc
      421 accgaagtgt tcataatgcc acctaccgtc gtctggtacg ccacgcccac ggtcagcgca
      481 cgaacaagtc caatcgtttc cacctgaaca gaacccacgt taatgatctg gttactgccc
      541 accgtctcta tctggttaac gcccaccgtc tgtatctgat tgttggtaac cgcaatcatc
      601 tgattgttgc caatggtctc agcatggttg tttatcacat ccgtggtgcg gttattcagc
      661 acctcggtgt tcatgttctt ctgcgcgtgg atgtagacct gttctttccc tgtcgcatcg
      721 tcaaacttca gttcattaaa cccgctgccc ttatagtttt tcgaacgaat ggtcatctgc
      781 gtcttcgtcc ccggcaggct gccgggtgtg cggttttcct ggtggtaggt acgccccata
      841 ataatcggct ggtccggatc gccgttgagg aagtccacaa tcacctcctg acccacacgc
      901 ggtatcgcca ggttgccaaa gccggtgcct gcccacgcct gtgccacacg gatccagcat
      961 gaactgtcct ggtttgacgg gttatagcgg tcccagttaa atttcacccg cacgcgacca
     1021 tgttcgtcgc agaagatttc ctcgcctgcc ggtcccgtca cgacggcgct ctgcgggcca
     1081 tccaccagcg gtttcagcaa cggctgtggt cgccatgtcc ggtctgccgg tattaccgca
     1141 aagtggttat tcagcgtggt acctgaaccc ctgcgtcccg gcaccgcctg tggctgctcg
     1201 ccgtgcagtt cacttgccac cacctgccat tcccggttca ggttcgcctg cggatgcccc
     1261 gtcagcacaa ttcgccgtcc cggccatatt tccggagaac ggcttgttcc gcgcgccact
     1321 tctgcgttgt tgcgccagcc atccatctgc cagcgggcaa agttctgccc gtgggcaccc
     1381 ttgaaacgtc cggggtagtc atacacttca tactgtgtgc gctggtaatc ctggtactgg
     1441 ccttcctgat caaaacgccc tgcccagccg ggtcgtttaa aggtgtagtc cttggtcacc
     1501 acggaagaag ggcggatttg tgcgctgtag cggaactggc tgatgcatag ggtgctcacc
     1561 tcggtacggg tgttcgggtt ccaggggatc tcaaaggact ccggcagata acgcacggtg
     1621 tcgcacagga ccaggctctg gtcgatactt ttttgcgcgt gctcctcata aaagaagatg
     1681 ccttcctccg ccgccatccg gcacaggaaa tcgtaatcag tttcgccgta ctggacacaa
     1741 aactcacggg aaggatgcgg ctcgctgaac agcggactcc actcggtcac accgttttct
     1801 ttcaggatcg tagcgagtat gctttcgatg tcctcattct ggaagatacg gaagttctgg
     1861 cgcagccctg tgcgccacag cggcgggcac accttcatgc tgtacagcat ctggtttttg
     1921 tcgttctccc ccagttcaaa ccaggtcacc acacctttca cccggcgctg tacgtcatcg
     1981 ccctgccata ccgtcaggta ggccattttg tccagcacct gcgcaaattc aagggaaaga
     2041 aactgctgac tgaccagaga aaggtcgagg gaaaaaagtg aagagagtga ctggttcaga
     2101 tgaaaggaga ccaccgcaaa cgcgtccggt ggcaggccgt ccacttccag cgtgaaacgt
     2161 aatccggttg acatagcaac ctccgtgaaa atgaataata atccgcacat attgcatgta
     2221 caacatgtcg ggcggatgaa ataagtgagt cgacatctgg cgttaccggg gaatatcaga
     2281 aaaatgtcat ggttatgtga tgatttagtc tgggatttta cttcagagaa acgggtgtct
     2341 gtttagtcaa caaaataatc acaaacgcaa cggatgggtt ctcgacgtta ttcacgacag
     2401 gctcgtacat tacagcgagt aataaaaaac ctgatatgca aagtcaaacc aatattcata
     2461 atgtcttttt attcacaaca ataaataatt taataacaat cattgaaatg aatgatgatt
     2521 tgtataagga atatacgcta ccgc
//
LOCUS       DAZFAB010000241         2480 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZFAB010000241 DAZFAB010000000
VERSION     DAZFAB010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.243"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            29..352
                     /gene="yiaW"
                     /locus_tag="ACHJWA_004883"
     CDS             29..352
                     /gene="yiaW"
                     /locus_tag="ACHJWA_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFO4311300.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQSHDSSVMQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            358..1494
                     /gene="yiaV"
                     /locus_tag="ACHJWA_004884"
     CDS             358..1494
                     /gene="yiaV"
                     /locus_tag="ACHJWA_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO4311301.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQKAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELNEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(1491..2465)
                     /gene="yiaU"
                     /locus_tag="ACHJWA_004885"
     CDS             complement(1491..2465)
                     /gene="yiaU"
                     /locus_tag="ACHJWA_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4311302.1"
                     /translation="MTKLQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADF
                     ESKVGLKVFDRTTRQLMLTLFGTALLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYA
                     PTGIITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDE
                     SLVASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGS
                     KEVRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWW
                     LDLVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
BASE COUNT          611 a          576 c          627 g          666 t
ORIGIN      
        1 gttctttttt atattctctg agggaaatat gttcctggac tattttgcac tgggagtgct
       61 tatttttgta tttctggtga tattctatgg gattattatt ttacatgata ttccctacct
      121 gattgctaaa aaacgtaatc atcctcatgc cgacgccatt catgttgctg gttgggtgag
      181 tctttttacg ctgcatgtta tctggccatt tttgtggatt tgggccacgc tttaccgccc
      241 ggagcgggga tggggaatgc aaagccatga ttcatccgtt atgcaactgc aacagcgcat
      301 tgccgggctg gagaaacagc tcgccgacat caaatcctct tctgccgagt aatacttatg
      361 gatttactga ttattttgac ctatgtggct tttgcatggg caatgtttaa gatcttcaaa
      421 attcccgtaa ataaatggac cattcccaca gcggccctgg gtggaatatt tattgtcagc
      481 ggtctaattc tgttaatgaa ctataaccat ccgtatacct ttaaagcgca aaaagcggtt
      541 atttctattc ctgttgtccc acaggtgaca ggcgtggtga tagaagtgac ggataagaaa
      601 aatacgctga ttaaaaaagg tgaggtgcta tttcgactgg acccgacgcg ttatcaggcg
      661 cgggtggatc ggctgatggc ggatatcgtt accgcagaac ataaacagcg ggcgttgggc
      721 gcagagttag atgagatggc ggcgaatact cagaaggcaa aggccacgcg ggataaattc
      781 gctaaggagt accagcgtta cgcacgcggc agccaggcga aagtaaaccc gttttcagaa
      841 cgcgatattg atgtggcacg gcaaaattat ctggcgcagg aagcctccgt aaagtcatcg
      901 gcggcggaac aaaaacagat ccagagccag ctggatagcc tggtgttggg tgaacattct
      961 caaatcgcca gcctgaaagc gcagctcgcg gaagcaaaat ataaccttga gcagacgata
     1021 gtgcgtgcgc cgagcgatgg ttatgtgacc caagtgctga ttcgtccggg tacctatgcc
     1081 gcgtcgctgc cgctacgtcc ggtgatggtg tttatacccg atcagaaacg acaaatcgtg
     1141 gcgcagttcc gtcagaactc cttgctgcgc ctggctcccg gcgatgatgc ggaagtggta
     1201 tttaacgctc tgccaggcaa agtgttcagc ggtaagctgg cagccattag tccagctgtt
     1261 cccggcggag cttatcagtc gaccggcacc ttacagacgt taaacacagc gccgggttca
     1321 gatggcgtta tcgcgaccat tgaactgaat gagcacactg atttgagcgc tttaccagac
     1381 ggtatttacg ctcaggtggc ggtctactct gatcatttca gccatgtctc ggtgatgcgc
     1441 aaagtgttat tacgcatgac cagctgggtg cattaccttt atctcgatca ttaatcttcg
     1501 ctttccgccg cttgttcagg ccgcagttgc tgcgactgag caagctggtg gcgaatttca
     1561 cgcagcattt catccagcac atactggacc cgccagggct ggtattcccg tttacggaag
     1621 atagccacca gatccagcca ccattcatac gggtgctgaa agcagggcac cagcgtgccg
     1681 tcttgcagat cgctctgcac acttttgtct ggcgcaaaca caatccccag gtgatttcgt
     1741 gccagctcca gcgccgattg cgtgttatcg caaacgtaat ttcccttcac gcgataatcc
     1801 cgcacctctt tactcccggc gacgttgaag cgccagatat tcgcgtcgtc gatcatcatc
     1861 gagtcgatca aaatacagga gtgatgttcg agttcgtcag gacggctaat aggatgtttc
     1921 tcaagatagc gctggctggc ataagcggtt acggcatatt gggtaataaa actggcaacc
     1981 agcgattcat cttttggtgg ggcatagctg attaaaacat cacaatcatc aggaaattcg
     2041 acgccttcat aaaaggcatt acgctcaaga ttgcaggttt ttagcgataa ggtgatatca
     2101 ccgatatctt taattttatc gattacatgt ttggataaat aggtaattat gccggttggg
     2161 gcgtagatgg tgacccgacc acgtttctca tgcttataat ctgcaataaa attattaagc
     2221 tgctcgtttc tgtccagcat gtcgttgatg tacggtaata gcgcggtgcc aaaaagtgtg
     2281 agcatcagtt gtcgggtggt tcggtcaaag acttttaaac ccacttttga ttcaaaatca
     2341 gcaagatatt tgctgacgtt ggcctgtgcg atgccaagta cagtcgcggc atggctgata
     2401 ttttcactgg cagcgattac cgagataatt tttaactccc ggtacttaag ttgtaatttt
     2461 gtcatcgcaa cagcccaatt
//
LOCUS       DAZFAB010000242         2448 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZFAB010000242 DAZFAB010000000
VERSION     DAZFAB010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.244"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(39..227)
                     /locus_tag="ACHJWA_004886"
                     /pseudo
     CDS             complement(39..227)
                     /locus_tag="ACHJWA_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            1407..>2448
                     /locus_tag="ACHJWA_004887"
     CDS             1407..>2448
                     /locus_tag="ACHJWA_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFO4311303.1"
                     /translation="MLYNIPCRIYILSTLSLCISGIVSTATATSSETKISNEETLVVT
                     TNRSASNLWESPATIQVIDQQTLQNSTNASIADNLQDIPGVEITDNSLAGRKQIRIRG
                     EASSRVLILIDGQEVTYQRAGDNYGVGLLIDESALERVEVVKGPYSVLYGSQAIGGIV
                     NFITKKGGDKLASGVVKAVYNSATAGWEESIAVQGSIGGFDYRINGSYSDQGNRDTPD
                     GRLPNTNYRNNSQGVWLGYNSENHRFGLSLDRYRLATQTYYEDPDGSYEAFSVKIPKL
                     EREKVGVFYDTDVDGDYLKKIHFDAYEQTIQRQFANEVKTTQPVPSPMIQALTVHNKT
                     DTHDKQYTQAVTL"
BASE COUNT          729 a          533 c          496 g          690 t
ORIGIN      
        1 aaccagcaaa cgggcattgt gttctgaaat ccaggctatt gattcaacgt cagcgacaga
       61 tctgcgctgt aaaagaactt gtaaaacaac gtaatagaat tatccggaat ggtggcgact
      121 atgcactaga acaccacaat ttcgctctct cggtaaggca ctgtcacagc attcaacaga
      181 atgtgacttg ccagatttat tagcaccacc agatgtttaa ccgggtaacc acgacccagt
      241 cggggatata ttccatcttc tcgctgacat catcaagaat aaaaaggttt attacactca
      301 gagcaacgtt cttcagcagg cgggataatt cttcaacatt tagaagatgt gttattaaag
      361 gcatacaaac tggacgtatt gtattttctt ttggtggttg atctaaggta gcagtttatc
      421 ctgatgcgct gagatttctg taatatctac gtcaacattc tcctcgaata gaaaatgcag
      481 ccccggcaag taattcacat tttttaacaa aacgttgtta gcgtgccatt ttcagaatct
      541 ctccaagaaa gcgaattctt ttctcaaatt ctgcattctc agacacagct ttctactcca
      601 tggtaatggc ccaaatacga agttgctcag gatcattagt ggtgcttaga agttaaataa
      661 ccatgcggag taccactcgt catcacgtat ttatatatca tgaagctatt gttgtgtttt
      721 gtacatcagt agatatattc tgatatactc cttttgctag acataacctt tcacctgctt
      781 gcaaagcttc tgtgttctga cattgccaaa ttgttgcaat tctgtatcca gccttctttc
      841 agtcatagct tcgggccgcg ataagactca ctgatctgac cctgattcct cttgcagact
      901 ttatagacca attaaaatgc agtttctgca ggtcaacgtc tgaccatcat tgtcatcact
      961 ctggccatta gagtaacctt ctgcattcat ccttttgtaa aaagtttata ttagtatcag
     1021 caattaaccg gacctgatac tgatatgagt cttaccgcat atacggtcaa tttcagcaat
     1081 taattacatt atccacgcca aagtatttgt catcacaatg atggtacctt ctttcagaca
     1141 ccattttttc aactccgttt tccacggacc gcactcttat gtcaagagtg cggtccgtgg
     1201 atacaaccag agaccgactg acacgagtca gaggaaacga cggatatgtt cagtcgtaaa
     1261 atatctatca aaaaacatga ttaaggtcaa aaatgtttga tatttacaat ttatgaagat
     1321 gacaataatt atagatatat gagaacataa atgaaaataa ttatcattac agcaatcatt
     1381 tgcactttgt attaatgagg gatgaaatgt tatataatat accttgtcga atttatatcc
     1441 tttccactct gtcattatgc atttctggga tagtttctac tgcaaccgca acttcttcag
     1501 aaacaaaaat cagcaacgaa gagacgctcg tcgtgaccac gaatcgttcg gcaagcaacc
     1561 tttgggaaag cccggcgact atacaggtta ttgaccaaca aacattgcag aactccacca
     1621 atgcctccat agccgataat ttgcaggaca tccccggagt agagataaca gacaactcct
     1681 tggcaggccg taaacaaatc cgcattcgtg gcgaagcatc ctcccgtgtt ttaattctca
     1741 ttgatggtca ggaggtaact tatcagcgcg ccggagataa ttatggtgtg ggactgttga
     1801 tagatgagtc tgcgctggag cgtgttgagg tagtgaaagg tccatattcc gtactgtacg
     1861 gttcacaggc aattggcggt attgttaact tcatcaccaa aaagggaggt gacaaacttg
     1921 catctggagt tgtgaaagct gtttataatt ccgcaacagc aggctgggaa gaatcaatcg
     1981 cggtccaggg gagcatcggt ggatttgatt atcgcatcaa cggtagttat tctgatcagg
     2041 gcaatcgtga tacgccggat ggacgtctgc cgaataccaa ctatcgtaac aatagtcagg
     2101 gtgtatggtt gggttataac tccgaaaacc atcgttttgg cctctcgctt gatcgctaca
     2161 gactcgcgac acaaacttac tatgaggatc cagacggaag ttatgaggca tttagtgtca
     2221 aaatacctaa acttgaacga gagaaagttg gggtattcta tgacacagac gtggacggtg
     2281 actatctaaa aaaaattcat ttcgacgcgt atgagcagac catccagcgc caatttgcca
     2341 acgaagtaaa aacgacacag cctgttccca gtccgatgat tcaggctctg accgttcata
     2401 acaagactga cacccatgat aagcaataca ctcaggcggt cacattgc
//
LOCUS       DAZFAB010000243         2444 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZFAB010000243 DAZFAB010000000
VERSION     DAZFAB010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.245"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            23..166
                     /gene="kil"
                     /locus_tag="ACHJWA_004888"
     CDS             23..166
                     /gene="kil"
                     /locus_tag="ACHJWA_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HFO4311304.1"
                     /translation="MDQTLMAIQTKFTIATFIGDEKMFREAVDAYKKWILILKLRSSK
                     SIH"
     gene            241..537
                     /locus_tag="ACHJWA_004889"
     CDS             241..537
                     /locus_tag="ACHJWA_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO4311305.1"
                     /translation="MNAYYIQDRLEAQSWTRHYQQIAREEKEAELADDMGKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            543..1328
                     /gene="bet"
                     /locus_tag="ACHJWA_004890"
     CDS             543..1328
                     /gene="bet"
                     /locus_tag="ACHJWA_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFO4311306.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKVLGFLKQKASEQKVAA"
     gene            1325..2005
                     /locus_tag="ACHJWA_004891"
     CDS             1325..2005
                     /locus_tag="ACHJWA_004891"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFO4311307.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVIE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVVERDEKYMASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            2002..2184
                     /locus_tag="ACHJWA_004892"
     CDS             2002..2184
                     /locus_tag="ACHJWA_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFO4311308.1"
                     /translation="MKHPHDNIRVGAITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEE
                     INNKRGAVCTKHLPLS"
     gene            2157..2348
                     /locus_tag="ACHJWA_004893"
     CDS             2157..2348
                     /locus_tag="ACHJWA_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFO4311309.1"
                     /translation="MHKASPVELRTSIGMAHSLAQIGVRFVPIPVETDEEFHTLAASL
                     SQKLEMMAAKAEANERDPA"
BASE COUNT          665 a          595 c          634 g          550 t
ORIGIN      
        1 aatcagccag gagtcccaaa aaatggatca aacacttatg gctatccaga ctaaattcac
       61 tatcgccact tttattggcg atgaaaagat gtttcgtgag gccgtcgacg cttataaaaa
      121 atggatatta atactgaaac tgagatcaag caaaagcatt cactaacccc ctttcctgtt
      181 ttcctaatca gcccggcatt tcgcgggcga tattttcaca gctatttcag gagttcagcc
      241 atgaacgctt attacattca ggatcgtctt gaggctcaga gctggacgcg tcactaccag
      301 cagatcgccc gtgaagagaa agaggcagaa ctggcagacg acatgggaaa aggtctgccc
      361 cagcacctgt ttgaatcact ctgcatcgat catttgcaac gccacggggc cagcaaaaaa
      421 gccattaccc gtgcgtttga tgacgatgtt gagtttcagg agcgcatggc agaacacatc
      481 cggtacatgg ttgaaaccat tgctcaccac caggttgata ttgattcaga ggtataaagc
      541 ggatgagtac agcactcgca acgctggcag ggaagctggc tgaacgtgtc ggcatggatt
      601 ctgtcgaccc acaggaactg atcaccactc ttcgccagac ggcatttaaa ggtgatgcca
      661 gcgatgcgca gttcatcgca ttgttgatcg tcgccaacca gtacggcctt aatccgtgga
      721 cgaaagaaat ttacgccttc cctgataagc agaacggcat cgttccggtg gtgggcgttg
      781 atggctggtc ccgcatcatc aatgaaaacc agcagtttga tggcatggac tttgagcagg
      841 acaatgaatc ctgtacatgc cggatttacc gcaaagaccg taatcatccg atctgcgtta
      901 ccgagtggat ggatgaatgc cgccgcgaac cattcaaaac ccgcgaaggc agagaaatca
      961 ccggcccgtg gcagtcgcat cccaaacgga tgttacgtca taaagccatg attcagtgtg
     1021 cccgtctggc cttcggattt gctggtatct atgacaagga tgaagccgag cgcattgtcg
     1081 aaaatactgc atacactgca gaacgccagc cggaacgcga catcactccg gttaacgatg
     1141 aaaccatgca ggagattaac actctgctga tcgccctgga taaaacatgg gatgacgact
     1201 tattgccgct ctgttcccag atatttcgcc gcgacattcg cgcatcgtca gaactgacac
     1261 aggccgaagc agtgaaagtt cttggattcc tgaaacagaa agcctctgag cagaaggtgg
     1321 ctgcatgaca ccggacatta tcctgcagcg taccggaatc gacgtgagag ctgtcgaaca
     1381 gggggatgat gcatggcaca aattacggct cggcgtcatc accgcttcag aagttcacaa
     1441 cgtgatagca aagccccgct caggaaagaa gtggcctgac atgaaaatgt cctacttcca
     1501 caccctgctt gccgaggttt gcaccggtgt ggctccggaa gttaacgcta aagcactggc
     1561 ctggggaaaa cagtacgaga acgacgccag aaccctgttt gagttcactt ccggcgtgaa
     1621 tgttattgaa tccccgatca tctatcgcga cgaaagtatg cgtaccgcct gctctcccga
     1681 tggtttatgc agtgacggca atggccttga gctgaaatgc ccgtttacct cccgggattt
     1741 catgaagttc cggctcggtg gtttcgaggc cataaagtcg gcttacatgg cccaggtgca
     1801 gtacagcatg tgggtgacac gaaaagatgc ctggtacttt gccaactatg acccacgtat
     1861 gaagcgtgaa ggactgcatt atgtcgtggt tgagcgggat gaaaagtaca tggcgagttt
     1921 tgacgagatg gtgccggagt tcatcgaaaa aatggacgag gcactggctg aaattggttt
     1981 tgtatttggg gagcaatggc gatgaagcat cctcacgata atatccgggt aggtgcgatc
     2041 actttcgtct actccgttac aaagcgaggc tgggtatttc ccggcctttc tgttatcaga
     2101 aatcccctga aagcacagcg gctggctgag gagataaata ataaacgggg agctgtatgc
     2161 acaaagcatc tcccgttgag ttaagaacga gtatcgggat ggcacatagc ctcgctcaaa
     2221 ttggagtcag gtttgtgcca ataccagtag aaacagacga agaatttcat acgttagccg
     2281 catccctttc acaaaagctg gaaatgatgg cggcgaaagc agaagcaaac gagagagacc
     2341 cggcatgaca acaacagaat gcatttttct ggcagcaggc ttcatattct gtgtgcttat
     2401 gcttgccgac atgggacttg ttcaatgaca cctcagcagg aaaa
//
LOCUS       DAZFAB010000244         2436 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZFAB010000244 DAZFAB010000000
VERSION     DAZFAB010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.246"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            130..762
                     /locus_tag="ACHJWA_004894"
     CDS             130..762
                     /locus_tag="ACHJWA_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4311310.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWTEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
     gene            1096..1905
                     /locus_tag="ACHJWA_004895"
     CDS             1096..1905
                     /locus_tag="ACHJWA_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO4311311.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRRGFK
                     IHNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNNKILKHIKSYLIML
                     SAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTIDGLKEFDKYKTRIL
                     DSGINCKKYPWLYFPLAKSSPGMIVMACNKNYNQHEVAGCLTLSYREVNRG"
     gene            1902..2381
                     /gene="yqeJ"
                     /locus_tag="ACHJWA_004896"
     CDS             1902..2381
                     /gene="yqeJ"
                     /locus_tag="ACHJWA_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000916036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeJ"
                     /protein_id="HFO4311312.1"
                     /translation="MIIKKNLLFILVFISGFILFTVYSYTAEKMIYNETCTANWVIFN
                     DQGRANLTIDFMYNKKNKTGTVALSGTWQQGNRESKSIRRNIEYTWVENYDTAHLTSK
                     KVNKFEIMDQVDDDRLAELIPDFYVFPEKSVSYNILKQGKHAFILSIGNRAIMHCAR"
BASE COUNT          848 a          418 c          442 g          728 t
ORIGIN      
        1 ttaatcagaa ttatagcaaa tacagattaa tcgcaatttt tcccagcaaa attacaaaat
       61 catggtgtat tgacatcatc aatcaccaat gaaattatgc aattatatac ggatagggag
      121 gttcttaaca tggggctttg tagtcgttat aaaagtctta catgcaatag ttgctcaatg
      181 cattgccaaa taatgccaga agagtcaccg cgtttgcagt actgcgcaaa ctcgtgtttt
      241 tgtatgtgga ccgaagaaag ttcatatttt aatcgtggtg tggtagaagg tatcttaaca
      301 aaaaaccaca atgcgagatt aagcggatat atttttgtcg atttttcagt aagtttttta
      361 cgtctattcc ttgaaaaaga ctggattgac tatcttgcaa gtactgacat ggggattgtt
      421 ttagtcagcg accgcaatat gcaatcgtta gctaattact ggcgaaaaca caactctgca
      481 atatctgctg tcatatataa tgatgatggt cttgatgttg ccaatgaaaa gatcagacaa
      541 ctatttattg gtcgttattt atcatttacc agaggtaaca cattaactca gatggaattc
      601 accataatgg ggtatatggt ttctggttat aatccatatc agattgctga agttctggat
      661 atggatatcc gtagcatcta cgcgtacaag caacgaatcg aaaagagaat gggtggtaaa
      721 ataaacgaat tatttattcg ttcacattcg gtccaacatt gatactcaac aatctgccag
      781 aatcatactc tgtttatacg ggcatttatt gaaatgcccg tattttataa ataggccgtc
      841 ccctcaagag taaacaccat agtatttaaa accatcacac gaaaaattca gaagcattac
      901 gaaatatgaa cttacaaaca atagtggcat aaatgttaac catgttaatt tacataaaat
      961 tttacgttgc gacattaaag cctcatttca atcatcatga taaatataaa attaatatat
     1021 atttatgccg taaataccaa tatacttagc aaactatgtg atttccattt cgatttattt
     1081 agtgtttatt gacgtatgta ctggattatt aacgataata tcgagttctg gcctgagcac
     1141 cgaaaattaa tatcggtaca taacgccgat cttaacgtcg ttctgacaac gccagccagt
     1201 cgttgtttat cactgctact tgaagctttt cctgatgtgg ttgcacaaca agattttttc
     1261 accagagtct gggaagaaga aggtatgcgt gtgcctacta acacgttata tcagaacata
     1321 tccattatca gacgcggatt tcgcgctgtt ggtgatacta cccactcgct tattgcaacc
     1381 gtgccgagaa gaggattcaa gatccataat gacattaaca tacaaaatca tgtaataaac
     1441 tcatcaacag acgcacatac acacaatgcc ccacctgcca taaaagttaa tgcgggatac
     1501 aaagagagca ttggtggcgc aaagaatttc aataacaaaa tcctcaaaca tataaaaagt
     1561 tatctaatta tgttgagcgc atttgtcata ggcgcatatt ctgcatattg gctatggaat
     1621 aataatcaac cgaagccatt tttcaaggat tacaagactg ttgcggaaat taatggctgc
     1681 cattttaatg taacagaaga tacaattgat gggttgaaag agttcgataa atataaaaca
     1741 cggatactgg attcggggat taactgcaaa aaatatccgt ggttatactt ccctcttgca
     1801 aaatcctcac cggggatgat tgttatggca tgcaataaaa actataacca acatgaagtg
     1861 gcaggctgtt taaccctgtc ttaccgtgag gttaatcgtg gttgattata aaaaaaaact
     1921 tattatttat tttagttttt atcagtgggt ttattctctt cactgtctat tcttacactg
     1981 cagaaaaaat gatatataac gaaacatgca ctgcaaattg ggtaattttt aatgaccagg
     2041 gacgagcaaa tcttaccata gactttatgt ataacaaaaa aaataaaaca ggaactgttg
     2101 cgttgagtgg aacctggcaa caaggtaaca gagaaagtaa atcaatacgg cgaaatattg
     2161 aatacacatg ggttgaaaac tatgacacag cccatttaac atcaaagaaa gttaataagt
     2221 ttgagattat ggatcaagtc gatgatgata gacttgcaga acttattcct gatttttatg
     2281 tctttccaga aaaaagtgta agctataata tactaaagca aggtaagcat gcttttattt
     2341 tgagcattgg taacagagca ataatgcatt gtgcaaggta aactaaaata gataacaaaa
     2401 agatggaacc tcgttaatca taaaacaata aaatta
//
LOCUS       DAZFAB010000245         2395 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZFAB010000245 DAZFAB010000000
VERSION     DAZFAB010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.247"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..182
                     /locus_tag="ACHJWA_004897"
     CDS             <1..182
                     /locus_tag="ACHJWA_004897"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311313.1"
                     /translation="CRIRLFFVHHRNVPENIYKKQAQKKRPRLLLPEALLHDKGVFIA
                     RMTSLNTASMAKMRQ"
     gene            301..495
                     /locus_tag="ACHJWA_004898"
     CDS             301..495
                     /locus_tag="ACHJWA_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311314.1"
                     /translation="MPENNTRKPDKSATVHIDAGTMEKIERYQQFIKDNHPGMPVPTK
                     GQITRSAVEYWYRATLGAWL"
     gene            492..2372
                     /locus_tag="ACHJWA_004899"
     CDS             492..2372
                     /locus_tag="ACHJWA_004899"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HFO4311315.1"
                     /translation="MKTWFSIKAMADVVYVRIYDEIGGYGVKASALTDEINACGNASE
                     IHLRIHSPGGDIFEGLAIYNALKNHPAKKIVHIEGMAASMASFIAMCGDHIVMPENAM
                     MMIHAPRGVTAGVSGDVRRFADLMDKLGDTMAETYAGRTGRSKQEITAMMEAETWMDG
                     NECKANGCADEVIPAITAMARIESKRIGDFSNMPENIKNMISQKTGSGEQERLNGIRE
                     LFGTFNGRYNDLAISCLADSECSVENARERLLLAMGKESTPTNKTTPANLYYAYTDNG
                     NITGDAMRQGLNARLGHERAERGNPYAMMSLFDMAQASLTHRGISTGSYGTRSQIVNA
                     AFNHSSSDFTYILAGGAEKSVLAGWEQSGETFRQWTKKGSLSNFHEARRVGLNGFSTL
                     NKVPEGAEYKYITTSDRGEPIALATYGNIFSITRQAIINDDLNQLTVIPQAMGRAAAR
                     TVGNLVHLVLTGNTKLSDGIPLFSKKHGNDIDNGVSTTGLGIARRAMRTQRDENGEVL
                     NIVPKFLLVPAALEDRALQMVNSTTPFGADTNSGSYNPYHKQLDIIVDPRLDEVSDKQ
                     WYMLSAQGTDTIEVAYLDGNDEPYLEQQEGFIVDGVAWKVRIDAGVAALDYRGMVRAI
                     SS"
BASE COUNT          689 a          544 c          616 g          546 t
ORIGIN      
        1 agtgtcgaat caggttgttt tttgttcatc accggaacgt tcccgaaaac atttacaaaa
       61 aacaggcgca aaaaaagcgc ccccgattgc tgttaccgga ggcgctttta cacgacaaag
      121 gagtttttat cgccaggatg acgagtctta atactgcttc aatggcaaaa atgcgtcaat
      181 aactttgcct ttctgagaat aatcagaaaa atcataatct gattttcagg tagaaaatga
      241 tttatctatt acttttatcg atcaataatg atgcccgtta atcaaaacgg aggcggattt
      301 atgccagaga acaacaccag aaagccggat aaaagtgcca cggtacacat agacgccgga
      361 actatggaga agatcgaacg ctatcagcag ttcatcaaag ataatcaccc gggtatgcca
      421 gtacccacga aaggacaaat cacacgcagc gcggttgaat actggtacag ggcaacgtta
      481 ggggcctggc tatgaaaaca tggttttcca ttaaggctat ggcagatgtt gtctatgtgc
      541 gcatttatga cgagatcggc gggtacggtg taaaagcatc ggcacttact gacgagatca
      601 acgcgtgtgg taatgcgtct gaaatccatc ttcgcatcca ttcacctggt ggcgacatct
      661 ttgaagggct ggctatctat aacgccctga aaaatcatcc ggcaaagaaa attgtacaca
      721 ttgagggcat ggcggcttct atggcctcgt ttattgccat gtgtggcgat cacatcgtta
      781 tgcctgaaaa cgcgatgatg atgatacatg ccccccgtgg tgttactgcc ggagtgtcgg
      841 gcgacgttcg ccgctttgct gacctgatgg acaagctggg cgacacgatg gcagaaacct
      901 acgccggaag aacgggcagg agtaaacagg aaatcaccgc catgatggag gcggaaacct
      961 ggatggatgg caatgagtgt aaggctaacg gttgcgcaga tgaggttata cccgcgatta
     1021 cagcaatggc ccgaattgaa tcaaaacgaa tcggagattt ttcaaatatg ccggaaaata
     1081 ttaaaaacat gatcagccag aaaactggca gcggagaaca ggaacgactc aacgggatac
     1141 gtgaattgtt tggcacgttc aacggaagat ataacgacct ggctataagt tgtcttgctg
     1201 attcagaatg tagcgttgag aatgcacgcg aacgcctttt actcgctatg ggtaaagaat
     1261 caacgccaac aaacaaaacc acccccgcaa atctttacta cgcgtacacg gataacggca
     1321 acataaccgg cgatgcaatg cgccaggggc ttaatgcgcg tcttggtcac gaacgggccg
     1381 aacgcggtaa tccttacgcc atgatgagcc ttttcgatat ggcacaggca tcattaaccc
     1441 atcgtggtat aagcacgggc agctacggca cacgctcgca gatagtaaac gcggcattca
     1501 accacagcag cagcgatttt acttatatcc ttgctggtgg cgctgaaaaa tccgtgcttg
     1561 ccggatggga gcaaagcggc gagacatttc gccagtggac gaaaaaaggt tcattgtcaa
     1621 acttccatga agcgcgtcgt gttggtctga atggcttctc gacattaaac aaagtgccgg
     1681 aaggtgcgga atataaatac ataaccacca gtgaccgtgg tgaaccaatc gcgctggcca
     1741 cctacggtaa tattttcagc attacccgac aggcgattat taacgatgat ctgaatcagt
     1801 taaccgttat cccgcaggca atggggcgag cagccgccag aacggtgggt aatctggttc
     1861 atctggtgct gacaggaaac acaaagcttt ctgacggtat accgctgttc agcaaaaaac
     1921 acggaaatga tattgataac ggagtcagca ctacagggct tggcattgca cgccgtgcaa
     1981 tgcgcacaca gagggatgaa aacggtgagg tgctgaatat tgtgccaaaa ttccttcttg
     2041 ttccagcggc tctggaagac agagcgttgc aaatggttaa ctcaaccacg ccttttggtg
     2101 ccgatacaaa cagtggttca tataacccgt accacaaaca actggatatt attgtcgatc
     2161 cccgccttga tgaagtcagt gataagcaat ggtacatgct ttcagcacag ggaacggaca
     2221 caattgaggt ggcttatctt gacggtaatg acgagcctta ccttgagcag caggagggct
     2281 ttattgttga cggtgtggcc tggaaggtcc gcattgatgc tggcgttgct gctctggatt
     2341 atcgcggaat ggtcagagcg atttcttcat gagaacaagg cggcagcagc cgcct
//
LOCUS       DAZFAB010000246         2339 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZFAB010000246 DAZFAB010000000
VERSION     DAZFAB010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.248"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            159..704
                     /locus_tag="ACHJWA_004900"
     CDS             159..704
                     /locus_tag="ACHJWA_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFO4311316.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AAIKWYAERDAEIENEKLRREVEELRQDSETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            679..>2339
                     /locus_tag="ACHJWA_004901"
     CDS             679..>2339
                     /locus_tag="ACHJWA_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO4311317.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADASYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFTNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSASVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDL"
BASE COUNT          572 a          568 c          673 g          526 t
ORIGIN      
        1 tgaaaatttt ccggtttaag gcgtttccgt tcttcttcgc cgtaacctaa tgtttttatt
       61 taaaacaccc cctgaaaaga aaggaaacga caggtgctga aaacgggctt tttggcctct
      121 gtcgtttcct ttctctgttt ttgtccgtgg aatgaacaat ggaagtcaac aaaaagcagc
      181 tggctgacat tttcggcgcg agtatccgta ccattcagaa ctggcaggag cagggaatgc
      241 ccgttctgcg gggtggtggc aagggtaatg aggtacttta tgactctgcc gccgccataa
      301 aatggtatgc cgaaagggat gctgaaattg agaacgaaaa gctgcgccgg gaagttgaag
      361 aactgaggca ggacagcgag acagatctcc agccagggac tattgagtac gaacgccatc
      421 gacttacgcg tgcgcaggcc gacgcacagg aactgaagaa tgccagagac tcagctgaag
      481 tggtggaaac cgcattctgt actttcgtgc tgtcgcggat cgcaggtgaa attgccagta
      541 ttctcgacgg gatccccctg tcggtgcagc ggcgttttcc ggaactggaa aaccgacatg
      601 ttgatttcct gaaacgggat atcatcaaag ccatgaacaa agcagccgcg ctggatgaac
      661 tgataccggg gttgctgagt gaatatatcg aacagtcagg ttaacaggct gcggcatttt
      721 gtccgcgccg ggcttcgctc actgttcagg ccggagccac agaccgccgt tgaatgggcg
      781 gatgccagtt actatctccc aaaagaatcc gcataccagg aagggcgctg ggaaacactg
      841 ccctttcagc gggccatcat gaatgcgatg ggcagtgact acatccgcga ggtgaatgtg
      901 gtgaagtctg cccgtgttgg ttattccaaa atgctgctgg gtgtttatgc ctacttcata
      961 gagcataagc agcgcaacac ccttatctgg ttgccgacgg atggtgatgc cgagaacttt
     1021 atgaaaaccc acgttgagcc gactattcgt gatattccgt cgctgctggc gctggccccg
     1081 tggtatggca aaaagcaccg ggataacacg ctcaccatga agcgtttcac caatgggcgt
     1141 ggcttctggt gcctgggcgg taaagcggca aaaaactacc gtgaaaagtc agtggatgtg
     1201 gcgggttatg atgaacttgc tgcctttgat gaggatattg aacaggaagg ctctccgacg
     1261 ttcctgggcg ataagcgtat tgaaggctcg gtctggccaa agtccatccg tggctccacg
     1321 cccaaagtga gaggcacctg ccagattgag cgtgcagcca gtgaatcccc gcattttatg
     1381 cgttttcatg ttgcctgccc gcactgcggg gaggagcagt accttaaatt tggcgataaa
     1441 gagacgccgt ttggcctcaa atggacgccg gatgatccct ccagcgtgtt ttatctctgc
     1501 gagcataatg cctgcgtcat ccgtcagcag gagctggact ttactgatgc ccgttatatc
     1561 tgcgaaaaga ccgggatctg gacccgtgat ggcattctct ggttttcgtc atccggtgaa
     1621 gagattgaac cgcctgacag tgtgaccttt cacatctgga cggcgtacag cccgttcacc
     1681 acctgggttc agattgtcaa agactggatg aagacgaaag gggatacggg aaaacgtaaa
     1741 accttcgtga acaccacgct cggtgagaca tgggaagcga agatcggcga acgtccggat
     1801 gctgaagtga tggcagagcg gaaagagcat tattcagcgt ccgttcctga ccgtgtggct
     1861 tacctgaccg ccggtatcga ctcccagctg gatcgctacg aaatgcgcgt atggggatgg
     1921 gggccgggtg aggaaagctg gctgattgat cggcagatta ttatgggccg ccacgacgat
     1981 gaacagacgc tgctgcgtgt ggatgaggcc atcaataaaa cctatacccg ccggaatggt
     2041 gcagaaatgt cggtatcccg tatctgctgg gatactggcg ggattgaccc gactattgtg
     2101 tatgaacgct cgaaaaagca tgggctgttc cgggtgatcc ccattaaagg ggcatccgtc
     2161 tacggaaagc cggtggccag catgccacgt aagcgaaaca aaaacggggt ttaccttacc
     2221 gaaatcggta cggataccgc gaaagagcag atttataacc gcttcacact gacgccggaa
     2281 ggggatgaac cgcttcccgg tgccgttcac ttcccgaata acccggatat ttttgatct
//
LOCUS       DAZFAB010000247         2335 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZFAB010000247 DAZFAB010000000
VERSION     DAZFAB010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.249"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..206)
                     /locus_tag="ACHJWA_004902"
     CDS             complement(<1..206)
                     /locus_tag="ACHJWA_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO4311318.1"
                     /translation="MKIKHEHIRMAMNAWAYPDGEKVPAAEIARTYFELGMTFPELYD
                     DSHPEALARNTQKIFRWLDKDTPDA"
     gene            complement(190..513)
                     /locus_tag="ACHJWA_004903"
     CDS             complement(190..513)
                     /locus_tag="ACHJWA_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4311319.1"
                     /translation="MKKDNYSFKRACAVVGGQSAMARLLGVSPPSVNQWIKGVRQLPA
                     ERCPAIERATKGGVLCEELRPDVDWTYLRRSSCYSQNMSMKQPNDENDHTRSIKRQMI
                     HENQT"
     gene            638..1114
                     /gene="racR"
                     /locus_tag="ACHJWA_004904"
                     /gene_synonym="rarC"
     CDS             638..1114
                     /gene="racR"
                     /locus_tag="ACHJWA_004904"
                     /gene_synonym="rarC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor RacR"
                     /protein_id="HFO4311320.1"
                     /translation="MLSGKDLGRAIEQAINKKIASGAVKSKAEIARHFKVQPPSIHDW
                     IKKGSISKDKLPELWRFFSDVVGPEHWGLNEYPIPTPSTSDTKSELLDINSLYQAASD
                     EKRAIVAFLLSGNATEPSWVDHDVRAYIAAMEMKVANYLKNQESKRKSQNITKTGT"
     gene            1430..1582
                     /locus_tag="ACHJWA_004905"
     CDS             1430..1582
                     /locus_tag="ACHJWA_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO4311321.1"
                     /translation="MDTLDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTESGARRW
                     LARNSD"
     gene            complement(1697..2212)
                     /locus_tag="ACHJWA_004906"
     CDS             complement(1697..2212)
                     /locus_tag="ACHJWA_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311322.1"
                     /translation="MIAELTAAMTAIRETAQIAKLMNEAKTQAEVNAAIGELNSKLAS
                     IQRECVSLVELVGTYQEINASLKAKIAEFENFEAQTEGYILSQLESGTFVYSKEVTVN
                     GGSIIMHLCPKCFGQKIVSILQPFPVREYEFFHKSRCLYCENQFLMNKNPDYVSPPSI
                     EELARKLNGNL"
BASE COUNT          671 a          552 c          497 g          615 t
ORIGIN      
        1 gcatcagggg tgtctttatc cagccaacgg aaaattttct gggtattacg agccagggct
       61 tccggatggc tgtcgtcgta cagttccggg aacgtcatcc ccagttcgaa ataagtccgg
      121 gctatttcag ctgcaggaac tttctcacca tcaggatatg cccaggcatt catcgccatg
      181 cggatgtgct catgtttgat tttcatgaat catttgcctc ttgatgcttc gggtatgatc
      241 gttttcgtca tttggttgct tcatcgacat attctgcgaa taacatgacg agcgtcgtaa
      301 gtatgtccaa tcaacatcag gacgaagttc ttcacacagg acaccacctt ttgttgctcg
      361 ttcaatcgca ggacatctct cagcaggcaa ctgacgtaca cctttgatcc attgatttac
      421 gcttggagga gatacaccta aaagcctagc cattgctgat tgcccaccga caacagcaca
      481 agctcgtttg aatgaatagt tatctttttt catcgaatga actccaaaaa acacacaata
      541 atattaggct tagcctaatg caattgtcaa taggctatgc ctaacgcttc gagagtaggt
      601 attgcctaat gcgatgcgca taggagacta ttaagcaatg cttagtggta aagacttagg
      661 ccgagcgata gagcaggcca ttaacaaaaa aattgcatca ggagccgtca aatcaaaggc
      721 ggaaatcgca cgtcatttca aagtccaacc accatcaatc catgactgga ttaagaaagg
      781 ttcgataagt aaagacaaac ttccagaact atggcgtttc ttttctgatg tggttggtcc
      841 agagcattgg gggcttaacg aataccccat accaacccca tccacttcag atacaaaaag
      901 tgaactttta gacataaaca gcctttatca agccgcctct gatgaaaaaa gagcaattgt
      961 ggctttcctc ttatctggaa atgctacgga gcctagttgg gttgatcatg acgttcgtgc
     1021 ctacattgcc gcaatggaaa tgaaggtagc taactatctg aaaaatcaag aatcaaaacg
     1081 gaaaagccag aacatcacca agacaggaac ttaaacttat atggtccgac gggaaattcc
     1141 tagttcccgt tagttaactc ctactacctc tcccacaaac catcacctat taggtcgcac
     1201 ccaaatcatt aggcgtagcc tattgacaag caattaggca tttcctatag ttttcccata
     1261 ccaacccatc ccgtcccaca caatacaggg caatacctag agttacccgg cagtggtcag
     1321 ggattaagta gccagcccga ggcgtaagaa catgacggca gggttcaact ttaactatgc
     1381 agcaggtttt tgttccgcta ccccggcgtt aaggggatat gaggtcagca tggatactct
     1441 tgatcttggc aacaacgaat ctctggtgtg cggcgtgttt cccaaccagg acggcacgtt
     1501 caccgcgatg acgtatacca aaagcaaaac gtttaaaacc gaatctggcg cgcgtcgctg
     1561 gttagcaaga aactctgact aatgaaatct ggtaattaag gaatcctcca cggggggaga
     1621 gtggtgcaca cgcgccggac acaagcaagc atccggcatg ctctttaaca atctggatat
     1681 ccataacagt aaaaatctac agattgccgt tcagttttct ggccaactct tcaatagatg
     1741 gaggtgatac gtaatccgga tttttattca taagaaactg attttcacag tacaggcacc
     1801 tacttttatg aaaaaattca tattctctaa ccgggaatgg ctgaagtatc gatactatct
     1861 tttgtccaaa acattttgga caaagatgca tgattatgct gccgccgttc acggttacct
     1921 ccttcgagta cacaaaagtt cccgactcaa gttggctaag gatataacct tccgtctgag
     1981 cctcaaagtt ttcaaattct gcaattttag ctttgagaga agcatttatt tcttgataag
     2041 tgcccaccag ctcaacgaga gacacgcatt cgcgctgaat agatgcaagc tttgagttca
     2101 gttcaccaat agccgcattt acttcagctt gagtttttgc ctcgttcatt agttttgcaa
     2161 tctgggcggt ttcacgaata gccgtcattg ctgccgttaa ttcagcgatc acattcaata
     2221 ctcttattgt tgttggggat atccagatta acaagatcct tgttgttggg gaataacggg
     2281 gaccacctcg cctgacgtgg taaaaagcag gcacacaaca cgaaagcaca cggcg
//
LOCUS       DAZFAB010000248         2331 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZFAB010000248 DAZFAB010000000
VERSION     DAZFAB010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.250"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(79..1491)
                     /gene="pykF"
                     /locus_tag="ACHJWA_004907"
     CDS             complement(79..1491)
                     /gene="pykF"
                     /locus_tag="ACHJWA_004907"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFO4311323.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(1499..1693)
                     /locus_tag="ACHJWA_004908"
     CDS             complement(1499..1693)
                     /locus_tag="ACHJWA_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311324.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            2048..2257
                     /gene="fumD"
                     /locus_tag="ACHJWA_004909"
     CDS             2048..2257
                     /gene="fumD"
                     /locus_tag="ACHJWA_004909"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFO4311325.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT          608 a          512 c          571 g          640 t
ORIGIN      
        1 ttgcttctgg ttatcgatta aataaaaaaa gcgcccatca gggcgcttcg atatacaaat
       61 taattcacaa aagcaatatt acaggacgtg aacagatgcg gtgttagtag tgccgctcgg
      121 taccagtgca ccagaaacca taactacaac gtcacctttg tgtgccagac cgctctgcag
      181 agccagttct ttacccagac ggtagaaatc atcagtagaa gtgatctctt taaccagctg
      241 cggcacaacg cctttgctca gtaccaactg atgagccgtt ttttcgttgg tggtcagtgc
      301 caggatggtg gcatccggga agtatttacg tactgcgcga gcagatttac cgccctgggt
      361 agcaaccacg atcagcggag catccagttt ttcagcagtt tcaacggcac cacggcatac
      421 cgcttcggta atgcgcagtt tacggttgtc attgttgaac tcgagacggc tgttcatcac
      481 gcggtcggta cgttcgcaga tggtcgccat gatagaaacc gcttccagcg ggtatttacc
      541 ttttgcggat tcaccagaca gcatcactgc gtcagtaccg tcgaggatgg cgtttgcaac
      601 gtcaccggct tctgcgcgag tcgggcgtgg gtttttgatc atggaatcca gcatctgggt
      661 cgcagtgata acgactttac gtgcacggat acatttttcg atcatcatct tctgggcgaa
      721 gataacttct tctaccggga tttctacacc caggtcgcca cgcgcaacca tgatgccgtc
      781 agaggcttcg aggatttcgt cgaagttgtt gaggccttcc tggttttcga ttttggagat
      841 gatgtggatg ttttcgccgc cgtgcgcttt caggtgctca cggatttcga taacgtcaga
      901 acgcttacga ataaaggaag cagcgacaaa gtctacgcct tgttcgcaac caaagatcag
      961 gtcctgtttg tctttttcag ccagtgctgg cagggcaatg gaaacgccag gcaggttcac
     1021 acctttgttt tcacccaggt cgccgttgtt cagcacttta cagataactt tgttaccttc
     1081 aatggcagta acttccatac cgatcagacc atcgtcaacc agtacggtgt tgccaacaga
     1141 caggtcagtt gtgaaacctt catacgttac cgcaaccatt tcgctgttgc cgataacaga
     1201 tttatcagta gtgaaagtaa aggtctgacc agctttcaga gaaacgtcgt taccgccttc
     1261 cagtttcatg gtgcggattt ccggaccttt ggtatcaagc aggatagcgg cggttttacc
     1321 agttttgctc atcacgttgc gcagattctg aatgcgctga ccgtgttctg catagtcacc
     1381 atgagagaag ttcagacgca taacgttcat gccagcgtcc agcattttag ctaacatctc
     1441 ttcagattcg gttttcggtc cgatggtgca aacaattttg gtctttttca tgacagtctt
     1501 agtctttaag ttgagaagga tgggagaaac ttgctttctg ggcgcactgc cggaaagcga
     1561 atccggtatt acgaaagtgg tggtgcctcg ctctaaggat aggtgacatc gaaaaagcgt
     1621 gcagaggaat gtgtgcttgt gtctcagccc aacgtgagtg cctgtttttg agtcgttgag
     1681 ttctacagtc caaagtgctg aaaccattca agagtcaatt ggcgcgcatt atacgctgaa
     1741 attaatccaa aaggaaaaca aaaacagcgt ttcaaaaaat atacaacttg ttagacaata
     1801 aattgttatc aaagatttaa cgttccaggg aaaaggttac gcaaaaactt cgctgtttgc
     1861 gcaacgtctg gtgccggggt tgtcactgtg aagataagtg aaagttcatc cggtgtataa
     1921 atacatcatc gctcaattgc tacagttaaa ataatttatc gcgttatttg tctgattttg
     1981 tttgattttt gtttaattgg ttagattgat atatgctgat agttggcatg ataaataggt
     2041 ataaagaatg ggaaatcgga caaaagagga tgaactgtac cgagaaatgt gcagagttgt
     2101 cggtaaagtt gtgctggaaa tgcgcgatct ggggcaggaa ccaaagcata ttgttatcgc
     2161 aggcgtatta cgtacagcat tagccaataa acgcattcag cgcagtgagc tggaaaagca
     2221 ggcgatggaa accgtcatta acgcactggt aaaatgacca ttcaggatga ggcgataagc
     2281 ttcatcctga tattcttgct tcagttgcgg tccagttaat cattctaaat a
//
LOCUS       DAZFAB010000249         2273 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZFAB010000249 DAZFAB010000000
VERSION     DAZFAB010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.251"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..291
                     /locus_tag="ACHJWA_004910"
     CDS             <1..291
                     /locus_tag="ACHJWA_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO4311326.1"
                     /translation="GLYPDAESYPWKSNAHYWLVTNLYQNMRANALTDAELRRKAADE
                     LTCMTARINRGETIPEPVKQLPVMGGRPLNRAQALAKIAEIKAKFGLKGASV"
     gene            288..590
                     /locus_tag="ACHJWA_004911"
     CDS             288..590
                     /locus_tag="ACHJWA_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HFO4311327.1"
                     /translation="MTGKEAIIHYLGTHKSFCAQDVAAVTGATVTSINQAAAKMARAG
                     ILVVDGKVWRTVYYRFATREEREGKVSTNLIFKECRQSAAMKRVLRVYKRTSMGTQ"
     gene            658..990
                     /gene="emrE"
                     /locus_tag="ACHJWA_004912"
     CDS             658..990
                     /gene="emrE"
                     /locus_tag="ACHJWA_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFO4311328.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAVIGMMLICAGVLVIN
                     LLSRSTPH"
     gene            1055..1177
                     /locus_tag="ACHJWA_004913"
     CDS             1055..1177
                     /locus_tag="ACHJWA_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311329.1"
                     /translation="MFLVFVYSFVPSRLLRKQIAIRLLLYIQDSVIVIVFLNKN"
     gene            1235..>2273
                     /locus_tag="ACHJWA_004914"
     CDS             1235..>2273
                     /locus_tag="ACHJWA_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO4311330.1"
                     /translation="MKKAIAYMRFSSPGQMSGDSLNRQRRLIAEWLKVNSDYYLDTIT
                     YEDLGLSAFKGKHAQSGAFSEFLDAIEHGYILPGTTLLVESLDRLSREKVGEAIERLK
                     LILNHGIDVITLCDNTVYNIDSLNEPYSLIKAILIAQRANEESEIKSSRVKLSWKKKR
                     QDALESGTIMTASCPRWLSLDDKRTAFVPDPDRVKTIELIFKLRMERRSLNAIAKYLN
                     DHAVKNFSGKESAWGPSVIEKLLANKALIGICVPSYRARGKGISEIAGYYPRVISDDL
                     FYAVQEIRLAPFGISNSSKNPMLINLLRTVMKCEACGNTMIVHAVSGSLHGYYVCPMR
                     RLHRCDRPSIKR"
BASE COUNT          691 a          383 c          544 g          655 t
ORIGIN      
        1 ggcctgtatc cggatgcaga gtcttatccg tggaaatcga acgcgcatta ctggttggtt
       61 accaacttgt accagaacat gcgggccaat gcgctgactg acgcggaatt acggcgcaag
      121 gctgccgatg aactgacctg tatgacagcg cgaattaacc gtggtgagac gatacctgaa
      181 ccagtaaaac aacttcctgt tatgggcggt agacctctaa atcgtgcaca ggctctggcg
      241 aagatcgcag aaattaaagc taagttcgga ctgaaaggag caagtgtatg acgggcaaag
      301 aggcaattat tcattacctg gggacgcata agagcttctg tgcacaggac gttgccgcgg
      361 taacaggtgc aaccgtaacc agcataaatc aggctgcggc taaaatggcg cgggcaggaa
      421 tcctggtcgt tgatggtaag gtctggcgaa cggtgtatta ccggttcgct accagagaag
      481 aacgggaagg aaaggtgagc acgaatctga tttttaagga gtgtcgccag agtgccgcga
      541 tgaaacgggt attgagggta tataaaagaa catcaatggg tacacaatga tgaaacaggt
      601 gagttgagtt caaactgtag tacaattctc tccagtttga acaggaaaga atatgttatg
      661 aacccttata tttatcttgg tggtgcaata cttgcagagg tcattggtac aaccttaatg
      721 aagttttcag aaggttttac acggttatgg ccatctgttg gtacaattat ttgttattgt
      781 gcatcattct ggttattagc tcagacgctg gcttatattc ctacagggat tgcttatgct
      841 atctggtcag gagtcggtat tgtcctgatt agcttactat catgggggtt tttcggccaa
      901 cggctggacc tgccagccgt tataggcatg atgttgattt gtgccggtgt gttggttatt
      961 aatttattgt cacgaagcac accacattaa aaataatttc ttttaaaaga ctgcaatatg
     1021 gcggttcata tctttacatg ggccgctttt gttaatgttt ttagtttttg tgtattcttt
     1081 tgtgccttca agattattgc gtaagcaaat tgcaatacga ttattgttgt atattcaaga
     1141 tagtgtgatc gtaattgtct ttttaaataa aaattaaaca aaaattatat ctcaccacta
     1201 aggtttataa aagcataagt tagcaggtgt caccatgaaa aaagccatag catatatgcg
     1261 attttcatca ccaggtcaga tgtctggcga ctcattaaac cgacagagaa gacttattgc
     1321 tgaatggtta aaggtaaata gtgattatta tcttgatacc ataacatatg aagatttagg
     1381 attaagtgca ttcaaaggaa agcatgcaca atcaggagct ttttcggaat ttttagatgc
     1441 tatagagcat ggttatatat tgccaggaac tacattgtta gttgaaagtc tggacagact
     1501 ttcaagagaa aaagtcggtg aagcgattga acgtctgaaa ttgattttga atcacggtat
     1561 tgatgttata actctttgcg acaatacagt ctataatatt gactctttga atgagccata
     1621 ttcattaata aaagccatac ttatagcaca aagggcaaat gaagaaagcg agataaagtc
     1681 aagtcgggtt aaattatcat ggaagaaaaa acggcaggat gcactggaat caggtacgat
     1741 tatgacggcg tcttgtccga gatggctctc cttagatgac aaaagaacgg cttttgttcc
     1801 agaccccgac agggtgaaaa ctattgagct aatttttaaa ctcaggatgg aaaggcgctc
     1861 attgaatgca atagccaagt atttaaatga tcatgctgta aagaatttct caggaaaaga
     1921 aagtgcatgg gggccttctg taattgaaaa attattagcg aataaagctc tgataggtat
     1981 atgcgtacct tcatatcgtg caagagggaa agggataagt gaaatcgctg gctattatcc
     2041 cagagtcata tcagatgatt tgttttacgc tgtacaggaa attcggttgg caccttttgg
     2101 tattagcaat agtagcaaga atcctatgct aataaatcta cttcgaacag ttatgaagtg
     2161 tgaggcttgt ggtaatacca tgattgttca tgcggtatct ggaagtttgc atggctatta
     2221 tgtttgtccg atgagaagat tacatcgatg tgacaggcca tcaataaaga gag
//
LOCUS       DAZFAB010000250         2263 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZFAB010000250 DAZFAB010000000
VERSION     DAZFAB010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.252"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(1..365)
                     /locus_tag="ACHJWA_004915"
                     /pseudo
     CDS             complement(1..365)
                     /locus_tag="ACHJWA_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
     gene            353..769
                     /locus_tag="ACHJWA_004916"
                     /pseudo
     CDS             353..769
                     /locus_tag="ACHJWA_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2303 family protein"
     gene            897..1433
                     /gene="yfdR"
                     /locus_tag="ACHJWA_004917"
     CDS             897..1433
                     /gene="yfdR"
                     /locus_tag="ACHJWA_004917"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFO4311331.1"
                     /translation="MSFIKTFSGKHFYYDRINKDDIDINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFNVIPLAPSHA
                     YGMFMERFNELSELHKCA"
     gene            1424..1774
                     /locus_tag="ACHJWA_004918"
     CDS             1424..1774
                     /locus_tag="ACHJWA_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001242737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eae protein"
                     /protein_id="HFO4311332.1"
                     /translation="MRMNVFEMEGFLRGKCVPRDLKVNETNAEYLVRKFDEVRAEARN
                     EGINYTASRLAAAFNHGFINKPLAEVFDVTRMILSAKEELANESHPIDGLSGEYAEKS
                     LEEWAERLRKGGSQ"
     gene            1771..2244
                     /locus_tag="ACHJWA_004919"
                     /pseudo
     CDS             1771..2244
                     /locus_tag="ACHJWA_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145689.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
BASE COUNT          597 a          501 c          569 g          596 t
ORIGIN      
        1 tccggtgcac gggagtcccg ggggaaagct ttgcccactg ttttaacgag atcgccttca
       61 atctggttcg cggacagttt tcccagaacc cgacagttct cgttaatcgt gacgttgttg
      121 agcgtcccgg agttcgcatt cacgttacca ctgatatccg catttttagc agtcagcttt
      181 ccatccggtg tcagggaaaa tgccggtgga tttccaccac tggtaatggt gggggccgtc
      241 aggcgcttca ggaacacgtc gttcatgaat atctggttgc cctgcgccac aaacatcggc
      301 gtttcattcc cgtttgccgg gtcaataaac gcgatacgat tggcggcaac cagaatggat
      361 tgaagactgg gccgactacc ttgtgggctt tgatgctaat ggtgacacca ttcaggcaac
      421 aaaagcggct gcggcggtcc gtaaaatcac gattgaagca aaccagaccg ctgattttga
      481 agataatgac ttcagcggca aacgctccct gatggagtct gtcgaagcga agaccaaaga
      541 cattatgcca gtggcatttg aatttaaatg cattccgttt gaaggtctga aagaacgtcc
      601 gtttaaatta cgcctcagca ttatcactgg cgatcgtccg gtactggttc tgcgcattat
      661 tcagctggag gcggtgcagg aagaaatggc taacgaattt cgtgatctgc ttgttgagaa
      721 attcaaggac agcaaagtag aaacctttat tggtactttc accgcctgat ttcattactg
      781 caaatgcccc tgagggggca tttatggaaa cgtaatttac tcaataatcg ccggatggtg
      841 agggattctt tttaccagaa ttcagcgcgg tgcagcgcat atacgtggag aacaaaatgt
      901 catttattaa aactttttcc gggaagcatt tttattatga caggataaat aaagacgaca
      961 tcgatattaa cgatatcgcg gtttcccttt caaatatctg tcgctttgcc ggtcatcttt
     1021 cgcacttcta cagcgtcgcc caacatgcgg ttctttgcag ccagctggta ccgcaggaat
     1081 ttgcttttga agcattaatg catgatgcaa cagaagcgta ttgccaggac atccccgctc
     1141 cactgaaacg ccttcttcct gactataaac ggatggaaga aaaaattgac gccgtaatcc
     1201 gtgagaaata cgggttaccc ccagttatga gtacgcccgt gaaatatgcc gatcttatca
     1261 tgctggcaac cgaacgccgc gatctcgggc ttgatgatgg ctctttctgg cctgtactgg
     1321 aaggcatccc ggcaacagag atgttcaacg tgattccact ggcacctagc catgcctacg
     1381 ggatgtttat ggaacgtttt aacgagttat cggagttaca caaatgcgca tgaatgtttt
     1441 cgaaatggaa gggtttcttc gcgggaaatg tgtaccgcga gatctgaaag tgaatgaaac
     1501 aaatgctgag tatctggtgc gtaaattcga tgaagtacgt gctgaggctc gcaacgaggg
     1561 tattaactat accgcaagcc gtcttgctgc tgctttcaat cacggattta tcaataagcc
     1621 tttggctgaa gttttcgacg ttacacgcat gattctgtca gcaaaagaag agttagctaa
     1681 tgaatcgcat ccgattgatg gcctgtccgg tgaatatgca gagaaatccc ttgaagaatg
     1741 ggcggaacgg cttcgcaaag gaggcagcca gtgactggac atgcagcaat cctcgacatg
     1801 tgctgtggca gtcgcatgtt ctggttcgat aagaatgacg accgggcgat atttagcgat
     1861 atcagaaagg aagagcacac attgtgtgat ggacgacgcc tgattatcag tcctgatctg
     1921 atagctgatt ttcgtgcact accatttgca gacgcatctt tttcgatggt tatattcgac
     1981 cctccgcatc ttgagcgtgt tggtgataac gcctggatgg gaaagaaata tggacggctg
     2041 aataaagata cctagcgtga tgatttgcgg cagggattta aagaagcctt tcgtgtgttg
     2101 cgtccatccg gcgttctgat ttttaaatgg aatgaaacgc aaatacctgt tcgccagata
     2161 ttggtactga ccgacagaaa acctgttatc ggtcaacgaa caggaaaaaa cgataaaacc
     2221 cactggatta tttttatgaa ataggcatcc agtgagtagg ttc
//
LOCUS       DAZFAB010000251         2256 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZFAB010000251 DAZFAB010000000
VERSION     DAZFAB010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.253"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            126..380
                     /locus_tag="ACHJWA_004920"
     CDS             126..380
                     /locus_tag="ACHJWA_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFO4311333.1"
                     /translation="MSQTENAVTSSSGAKRAYRKGNPLSDAEKQRLSVARKRASFKEV
                     KVFLEPKYKAMLMQMCHEDGLTQAEVLTALIKSEAQKRCV"
     gene            616..690
                     /gene="tap"
                     /locus_tag="ACHJWA_004921"
     CDS             616..690
                     /gene="tap"
                     /locus_tag="ACHJWA_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFO4311334.1"
                     /translation="MLGKVQDFFLCSLLLRIVSAGWCD"
     gene            683..1540
                     /gene="repA"
                     /locus_tag="ACHJWA_004922"
     CDS             683..1540
                     /gene="repA"
                     /locus_tag="ACHJWA_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFO4311335.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSKGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTPALFAALDV
                     SEDAVAAARRSRVEWENRLRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDAGRERQDIVTLVKRQLTREISEGRFTANREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
BASE COUNT          588 a          597 c          566 g          505 t
ORIGIN      
        1 acgacagtaa tccccgttga tttgtgcgcc aacacagatc ttcgtcacaa ttctcaagtc
       61 gctgatttca aaaaactgta gtatcctctg cgaaacgatc cctgtttaag tattgaggag
      121 gcgagatgtc gcagacagaa aatgcagtga cttcctcatc tggcgcaaaa cgagcataca
      181 gaaaggggaa tccgctttct gatgcagaga aacaaagatt atcagtggcc cgtaaaagag
      241 cttcgttcaa ggaagtaaaa gtatttcttg aaccaaagta taaggccatg ctcatgcaaa
      301 tgtgtcatga agatggcctg actcaggctg aagttctgac cgcactgata aaaagtgaag
      361 cgcaaaaacg atgtgtgtga tgatgggctt acattcttga gtgttcagaa gattagtgct
      421 agattactga tcgtttaagg aattttgtgg ctggccacgc cgtaaggtgg cagggaactg
      481 gttctgatgt ggatttacag gagccagaaa agcaaaaacc ccgataatct tcagaaagtt
      541 tggcgactga gaagattacc ggggcccact taaaccgtat agccaacaat tcagctatgc
      601 ggggagtata gttatatgct cggaaaagtt caagacttct ttctgtgctc gctccttctg
      661 cgcattgtaa gtgcaggatg gtgtgactga tcttcaacaa acgtattacc gccaggtaaa
      721 gaacccgaat ccggtgttca caccccgtga aggtgccgga acgctgaagt tctgcgaaaa
      781 actgatggaa aaggcggtgg gcttcacctc ccgttttgat ttcgccattc atgtggcaca
      841 tgcccgttcg aagggactgc gtcggcgcat gccaccggta ctgcgtcgcc gggctattga
      901 tgcgctgctg caggggctgt gttttcacta tgacccgctg gccaaccgtg tccagtgctc
      961 catcaccaca ctggccattg agtgcggact ggcgacggag tctgctgccg gaaaactctc
     1021 catcacccgt gccacccggg ccctgacgtt cctgtcagag ctgggactga ttacctacca
     1081 gacggaatat gacccgctta tcgggtgcta cattccgacc gacatcacgt tcacaccggc
     1141 tctgtttgct gcccttgatg tgtctgagga tgcagtggca gctgcgcgcc gcagccgtgt
     1201 tgaatgggaa aacaggctgc gcaaaaagca ggggctggat accctgggta tggatgaact
     1261 gatagcgaaa gcctggcgtt ttgtgcgtga gcgtttccgc agttaccaga cagagcttaa
     1321 gtcacgggga ataaagcgtg cccgtgcgcg tcgtgatgcg ggcagggaac gtcaggatat
     1381 cgtcaccctg gtgaaacggc agctgacgcg tgaaatctcg gaagggcgct tcactgctaa
     1441 tcgtgaggcg gtaaaacgcg aagtggagcg tcgtgtgaag gagcgcatga ttctgtcacg
     1501 taaccgcaat tacagccggc tggccacagc ttccccctga aagtgacctc ctcagaataa
     1561 tccggcacgc gccggaggca ttcgcacgcc tgaagcccgc cggtgcacaa aaaatcagca
     1621 ccacatccca aaaacaacct catcatctac cttctggtgc atccggtccc ccctgttttg
     1681 atacaaaaca cgtctcacag acgggcaatt ttgtttatcc acattaaact gcaagggatt
     1741 tctctgtaag attacaaccg ttcatgtcat aaagcaccag ccgccaggct tacagggtgc
     1801 aatgtatctt ttaaacaccc gtttatatct cctttaaact acttaattac attcatttaa
     1861 aaaaaagact tattcactgc ctgtcctgtg gacagacaga tatgcacctc ccaccgcaag
     1921 cggcgggccc caaccggagc cactttagtt acaacacaca aaaacaacct ccagaaaaac
     1981 cccggtcccg cgcagaactg aaaccacaaa gcccctccct cataactgaa aaacggcccc
     2041 gccccggccc aaagggccgg aacagagtcg cttttaatta tgaatgttgt aactaagcat
     2101 catcatcgct gtcagtcttc tggctgaacg ttctcagtac acgctcgtaa gcggccctga
     2161 gggctcgcta acgcggagat acgccccgac ttcgggtaaa ccctcgtcgg gaccactccg
     2221 accgcgcaca taaactctct catggctgaa agcggg
//
LOCUS       DAZFAB010000252         2253 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZFAB010000252 DAZFAB010000000
VERSION     DAZFAB010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.254"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(140..784)
                     /locus_tag="ACHJWA_004923"
     CDS             complement(140..784)
                     /locus_tag="ACHJWA_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFO4311336.1"
                     /translation="MESLGFRLKRLRKDKGLTQVELGKLSGVTGVTIGYWEKDLNEPG
                     SKALSKLAQALGTTESYLLYGVSSPELSFVQSTSGTKIPYLSWGEAISFLILKGEKTM
                     GNVDRITTFFDVEEGDFAVSMPDDTMHNPSGSPSIPVGATVILRPGESYKNGSIVAVI
                     VPDPLKNEPSMTIKKLVIDGKLVYLSPLNPRYQSSLLTPECKIVAVAKGVQFNL"
     gene            882..1109
                     /locus_tag="ACHJWA_004924"
     CDS             882..1109
                     /locus_tag="ACHJWA_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO4311337.1"
                     /translation="MRVDELVQFFGSVQRVADFYGITREAIYMWRKRPGEIVPKGRAA
                     EAAAYSKGKLSLNPELYKKKDTTQGEGKGDS"
     gene            1106..1531
                     /locus_tag="ACHJWA_004925"
     CDS             1106..1531
                     /locus_tag="ACHJWA_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO4311338.1"
                     /translation="MKIKHEHIRMAINAWAYPDGEKVPAAEIARTYFELGMTFPELYD
                     DSHPEALARNTQKIFRWLDKDTPDAVEKIQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVAAAIAWGTLTNSGGQPGNAVVVH"
     gene            1600..>2253
                     /locus_tag="ACHJWA_004926"
     CDS             1600..>2253
                     /locus_tag="ACHJWA_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HFO4311339.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTTCHGVSLRGHIDVTTEDLASALDVTEDVIDSILHAMQGRVLDGDLISGWEKRQV
                     MKEDNGNVSQTAKSPAERKRAQRERERLRKQNTDCHDESRRVTHMSRQITTDTDTDKE
                     LNPTHNARMRESATTGELNDASLQTAEPEYLDGLSEPIGKFPMTGVWQPSPDFRQRAA
                     "
BASE COUNT          615 a          485 c          578 g          575 t
ORIGIN      
        1 gtattgcccg acgttctgtg gggcgaggta gggtggtatg taataaatct aaaagtattt
       61 agtttttcag tcaaggtaaa tctagattaa tttaaaaaaa aggtcggcat tgccgacctc
      121 aagtaaagga cgtggcgggt tataagttga actgtacacc ttttgctacg gcaacaattt
      181 tacactctgg tgtaagtaag gatgactgat agcgtggatt gagagggctt aaatacacaa
      241 gcttcccatc aataactaat ttctttatag tcatagatgg ttcattttta agcggatccg
      301 gaactattac agcgacgatg ctgccatttt tataactttc tcctggcctt aggatcacag
      361 tagcaccaac tgggatactc ggtgatcctg atgggttatg cattgtgtca tcaggcattg
      421 aaacggcaaa atcaccttcc tcgacatcaa agaatgtggt gattctatcg acatttccca
      481 ttgttttctc tccttttaag attaggaaag aaatcgcctc accccacgaa aggtagggga
      541 tcttggtgcc tgaggtactt tgtacaaaag ataattcagg agacgatact ccatatagga
      601 gataggactc agtagttcct aatgcctggg ctaacttact tagagctttg ctacctggtt
      661 cgtttagatc tttctcccag taccctatag taaccccagt cacgcctgaa agcttaccca
      721 gttctacttg ggtcagcccc ttatcttttc taagtctctt aagcctgaag ccaaggcttt
      781 ccatcatttt ctcctgcaaa ttgaatataa attattttag attgcattga cctaaaaaaa
      841 attatcttgt aatctaaaaa tgcttagatt taggaggtaa aatgcgagtt gatgaacttg
      901 tccagttttt tggttctgtt cagagggttg ccgattttta tgggataacc cgcgaagcta
      961 tttacatgtg gcgtaagcgt cctggtgaaa tagttccgaa ggggagggct gcggaagctg
     1021 ctgcatactc caaggggaaa ttatctttga acccagaact ttacaaaaag aaggatacca
     1081 ctcagggcga aggaaagggg gattcatgaa aatcaaacat gagcacatcc gcatggcgat
     1141 caatgcctgg gcctatccgg acggtgagaa agttcctgca gctgaaatag cccggactta
     1201 tttcgagctg gggatgacgt tcccggaact gtacgacgac agccatccgg aagccctggc
     1261 tcgtaatacc cagaaaattt tccgttggct ggataaagac acccctgatg ctgttgaaaa
     1321 aattcaggct ctgttaccgg cgatcgaaaa ggcaatgccg cctctgctgg tggcccgtat
     1381 gcgcagtcac agctctgaat attaccgtga gatcgtcgaa cggagggatc ggctggtgaa
     1441 agatgtggat gattttgtcg cagcggcgat cgcctggggc accctgacta acagtggtgg
     1501 tcagcctggt aatgctgttg tcgtgcattg accaacaata ttcatgccgg atttcttccg
     1561 gatgttcgag ggtaaagttc ggtatcagat gaggtgagta tggctaatgc ctggctcaga
     1621 ttgtggcatg acatgccaaa tgatcccaaa tggcgaacca ttgccagggt ctcaggacag
     1681 ccaatcgcaa cagtgatggc ggtgtatatc caccttctgg tgagtgcgtc acgaaatgtc
     1741 acgacatgtc acggcgtgtc actacgtggt cacattgatg tcacgacgga agatttagca
     1801 agtgcgcttg atgtgacgga agacgtgatt gattcaattt tgcatgcaat gcaggggcga
     1861 gttctggatg gtgatcttat ttccggatgg gaaaaacgcc aggtgatgaa ggaggataac
     1921 ggtaatgttt cgcaaaccgc aaaatccccg gcagagcgca agagagcgca gcgggagcgg
     1981 gaaagactgc ggaagcagaa cactgattgt cacgatgagt cacgacgcgt cacgcatatg
     2041 tcacgacaaa tcacgacaga tacagataca gataaagaat taaaccccac acataacgcg
     2101 cgcatgcgcg agagtgctac gaccggtgag ttgaatgacg cgtcgttgca gacagccgaa
     2161 cctgaatacc tggacggcct gagcgaaccg atcgggaaat ttccgatgac tggagtctgg
     2221 caaccgtcgc cggattttcg acagcgggca gca
//
LOCUS       DAZFAB010000253         2239 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZFAB010000253 DAZFAB010000000
VERSION     DAZFAB010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.255"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>2239
                     /locus_tag="ACHJWA_004927"
     CDS             <1..>2239
                     /locus_tag="ACHJWA_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4311340.1"
                     /translation="DNGVGFRYSFSALKDRHTAVEVNYTDPQNGWQTSTELVEDPEAI
                     LRYGRNLLKMDAFGCTSRGQAHRAGLWVIKTGLLETQTVDFTLGSQGLRHTPGDIIEI
                     CDNDYAGTMTGGRVLSIDAASRTLTLDREVTLPETGAATVNLINGSGKPVSVDITAHP
                     APDRIQVSTLPDGVETYGVWGLSLPSLRRRLFRCVSVRENTDGTFAITAVQHVPEKEA
                     IVDNGARFEPQSGTLNSVIPPAVQHLTVEVSAADGQYLAQVKWDTPRVVKGVRFSLRL
                     TSGSGEGSRLVTTAITADTEHRSSGLPPGEYTLTVRAINSYGQQGEPATTTFRINAPA
                     VPATIELTPGYFQITAVPRLAVYDPTVQFEFWFSETKIADTSQVETSARYLGTGSQWS
                     VSGPHIKPGKDFWFYVRSVNLVGKSAFVEASGRASNDAEGYLGLFREKIGKLHLAQGL
                     WELIDNSQLADEMAEMKTSITETRNEITQTVSKTLEDQSAIIQQIQRVQKDTNDDLAA
                     LYMLKVQKTKNGIPYVAGIGAGIEDTDGQPLSNILLLADRIAMINPEDGNTTPLFVAQ
                     GNQLFMNDVFLKRLFAVSITSSANPPTFSLTPEGRLTARNADISGNVNANSGTLNNVT
                     INENCRVLGKLSANQIEGDLVKTVGKAFPRDSRAPERWPSGTITVRVYDDQPFDRQIV
                     IPAVAFSGAKHEREHTDIYSSCRLIVRKNGAEIYNRTALDNTLIYSGVIDMPAGHGHM
                     TLEFSV"
BASE COUNT          520 a          599 c          684 g          436 t
ORIGIN      
        1 atgataacgg cgtggggttt cgctacagct tcagcgccct gaaggaccgc cacacggcgg
       61 tggaggtgaa ttacaccgac ccgcagaacg gctggcagac ctccacggaa ctggtggaag
      121 acccggaagc catactgcgc tacggacgca acctgctgaa gatggatgcg ttcggctgca
      181 ccagtcgcgg tcaggcccac cgtgccgggc tgtgggtgat aaagaccgga ctgctggaaa
      241 cgcagacggt ggatttcacg ctcgggtcac aggggctgcg tcacacaccc ggtgacatta
      301 ttgaaatctg tgataacgac tatgccggga ccatgaccgg cggacgtgtc ctgtccatcg
      361 atgccgccag ccgcaccctg acgctggacc gtgaggtgac actgccggag accggtgccg
      421 ccacggtgaa cctgattaac ggcagcggta agccggtgag tgtggacatc accgcacacc
      481 ccgcgccgga ccggatacag gtcagcaccc tgcctgatgg tgtggagaca tacggtgtat
      541 ggggactatc cctgccgtca ctgcgccgtc gcctgttccg ctgtgtctcc gtccgggaaa
      601 acacggacgg cacctttgcc atcacggcgg tgcagcacgt accggaaaaa gaagccatcg
      661 tggataacgg tgcccgcttt gagccgcagt caggcaccct gaacagcgtc atccctccgg
      721 cagtgcagca cctgacggtg gaggtgagcg cagctgacgg ccagtatctg gcgcaggtga
      781 aatgggacac gccgcgggtg gtgaagggcg tgcgcttcag tctgcgcctg accagcggaa
      841 gcggagaagg cagccgtctg gtgaccaccg ccatcactgc ggatacagag catcgttcca
      901 gtggtctgcc gccgggggaa tacaccctga cggtcagggc gattaacagt tatggccagc
      961 agggggaacc ggccaccacc acgttcagga ttaatgcacc tgcggtaccc gccacgattg
     1021 agctgacacc gggctatttt cagataacag cggtcccgcg tcttgcggtg tatgacccga
     1081 cggtacagtt tgagttctgg ttttcggaga caaaaatcgc agatacatct caggtggaaa
     1141 cctctgcccg ttatctgggg accggcagtc agtggagtgt atccggcccg cacattaagc
     1201 ccgggaagga tttctggttt tacgtgcgca gcgtcaacct ggtggggaaa tctgcttttg
     1261 tggaagccag tggccgggcc agcaatgatg cagaagggta tctggggctg tttcgggaaa
     1321 aaataggaaa actgcatctg gctcaggggc tgtgggagct gatagacaac agccagcttg
     1381 cggatgagat ggcggagatg aagacctcca tcaccgaaac ccgcaatgaa atcacacaga
     1441 cggtcagtaa aacgctggag gaccagagcg ccatcataca gcagatacag cgcgtgcaga
     1501 aggacacaaa tgatgacctt gctgcacttt acatgctgaa ggtacagaaa acaaaaaatg
     1561 gcatacccta tgttgccggt attggagcgg ggattgagga tactgatggc cagcccctga
     1621 gcaacatact gctgctggct gaccgtattg cgatgattaa cccggaggac ggcaacacca
     1681 cgccgttatt tgtggcgcag gggaatcagt tgttcatgaa cgatgtgttc ctgaaacgac
     1741 tgtttgcggt gagtatcacg tcatccgcca atcccccgac gttttccctg acgccggagg
     1801 gcaggctgac cgcaagaaat gctgatatca gcggtaacgt gaatgcgaat tccgggacgc
     1861 tcaacaacgt cacgattaac gagaactgtc gggttctggg aaaactgtcc gcgaaccaga
     1921 ttgaaggcga tctcgttaaa acagtgggca aagctttccc ccgggattcc cgtgcaccgg
     1981 agcggtggcc atcagggacc attaccgtca gggtttatga cgatcagccg tttgaccggc
     2041 agattgttat tccggcggtg gcattcagcg gcgctaaaca tgagagagag catactgata
     2101 tttactcctc atgccgtctg atagtgcgga aaaacggtgc tgaaatttat aaccgtaccg
     2161 cgctggataa tacgctgatt tacagtggcg ttattgatat gcctgccggt cacggtcaca
     2221 tgacgctgga gttttcggt
//
LOCUS       DAZFAB010000254         2231 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZFAB010000254 DAZFAB010000000
VERSION     DAZFAB010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.256"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            19..510
                     /gene="ygeG"
                     /locus_tag="ACHJWA_004928"
     CDS             19..510
                     /gene="ygeG"
                     /locus_tag="ACHJWA_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO4311341.1"
                     /translation="MDTETIEIFNNSDEWANQLKHALSKGENLALLHGLTPDILDRIY
                     AYAFDYHEKGNVTDAEIYYKLLCIYAFENHEYLKGFASVCQSKKKYQQAYDLYKLSYN
                     YSPYDDYSVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDASVKSKAQAYIELLTDNSE
                     DNG"
     gene            844..2220
                     /gene="ygeH"
                     /locus_tag="ACHJWA_004929"
     CDS             844..2220
                     /gene="ygeH"
                     /locus_tag="ACHJWA_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
                     /protein_id="HFO4311342.1"
                     /translation="MDLENKFSYHFLEGLTLTEDGILTQGNEQVYIPPKELGVLIVLL
                     ESAGHVVLKDMIIESVWKNIIVSDESLTRCIYSLRCIFEKIGYDRCIETIYRKGYRFS
                     GQVFKTKINEDNTSDYSIAIFPFTTSLKTLDPLILNQELVQIISNKKIDGLYTYPMAA
                     TNFCNDHISQNSFLRRFNPDYFVTGRINQNNAVNTLYIELTDAKNLFLIASNHLPVDE
                     LHNTSQFIIENILQTVHNPQRSVRLTKQDQGYKNHYLSDEMLAGKKELYEFTPESIYR
                     AMTIFDGLQNKSDIQTLKTECYCLLAECHMSLALHGKSELELAAQKALELLDYVSDIT
                     TVDGKILAIMGLITGLSGQAKVSHILFEQAKIHSTDIASLYYYRALVNFHNEKIEEAR
                     ICIDKSLQLEPRRRKAVVIKECVDMYVPNPLKKNMKLYYKETESGSHRVIIDNILKLK
                     QLTRICMR"
BASE COUNT          822 a          370 c          376 g          663 t
ORIGIN      
        1 acatcacaga ggaatattat ggacacagaa acaattgaaa tattcaataa tagtgatgaa
       61 tgggcaaatc aactaaaaca cgcattatcg aaaggagaaa atctggcatt actacatggt
      121 ttgactcctg atatccttga tagaatatat gcatatgcat tcgactacca tgaaaagggt
      181 aatgtaacgg acgcagaaat ttattataaa ttattgtgca tttatgcgtt cgaaaatcat
      241 gaatatctaa aaggttttgc atcagtatgt cagtcaaaaa agaaatatca acaagcatat
      301 gacctttaca agctaagtta caattactcc ccgtatgatg actattcagt tatttatcgt
      361 atgggtcaat gtcaaattgg ggctaaaaat atcgataacg caatgcaatg tttctatcat
      421 attattaaca attgtgagga tgctagtgtt aagagtaaag cgcaggcata tattgaactc
      481 ttaaccgata attcagaaga taatggctaa gcattacaat catttggtta aaaatgctgg
      541 ttatagtgta gtaaccacta tgaccagcac ctcttatgct ttcttaaatc aacatcttaa
      601 aacagttaag aacacactaa aaaatattat ttttcaacgc gttagagaac aatctatttt
      661 atcaaaattg aataaggcta tttcagatta catcagtgaa ttcctaagat aaattagtga
      721 acacaaccat agatatgtcg aatgtaaact cctgtagcgt taatgacaca taaatatgaa
      781 tagccataat ttctattgct tatgcaggat gcaagaaacc aattttttca tagaggttaa
      841 ctaatggact tagaaaataa attctcatat cattttcttg agggattaac gctcacggaa
      901 gatggaattc ttactcaagg aaatgagcaa gtttatattc caccgaaaga gttaggtgta
      961 ttaatagtat tacttgaatc cgctggtcat gtcgtactga aagatatgat catcgaatca
     1021 gtatggaaaa atattattgt tagtgatgag tccctgacaa gatgtatcta ttctttgcgc
     1081 tgcatttttg aaaaaattgg ctatgatcgt tgcatagaaa caatctaccg gaaaggttat
     1141 cgtttcagtg ggcaggtttt caaaactaaa ataaatgaag ataacacttc agactattct
     1201 atcgctatat tccctttcac tacttcattg aaaacactgg atccattaat acttaatcag
     1261 gaattagtac aaatcatttc aaataaaaaa atcgatggcc tctataccta tccgatggct
     1321 gcgacaaatt tttgtaatga tcacatatcg caaaattcat ttttgagaag attcaatcca
     1381 gattatttcg ttacaggaag aataaaccag aataatgcag tgaacacttt atacattgag
     1441 ttgaccgacg ctaaaaacct tttcctcatc gccagtaatc atctacctgt tgatgaacta
     1501 cataatacat cacaatttat tatagaaaat atccttcaaa cagtacataa tccacaacga
     1561 tctgtaagat taactaagca ggaccaagga tataagaatc attatttatc agatgaaatg
     1621 ttagccggaa agaaagaact ttacgagttc acccctgaaa gcatttacag ggccatgact
     1681 atatttgatg gattacaaaa taaaagtgat atacagacgc taaaaacaga atgttattgc
     1741 cttctagcgg aatgccatat gtctttggca cttcatggaa aaagtgaact tgaacttgct
     1801 gctcaaaaag cattagagct tttagattat gtatcagaca taaccactgt cgatggaaaa
     1861 attttagcta ttatgggact gataactggt ctgtctggac aagcaaaagt atctcatatc
     1921 ttatttgaac aggctaagat acactcaact gatatagcct ctctctacta ctatagggca
     1981 cttgtcaact ttcataatga aaaaattgaa gaggcaagga tttgtataga caaatcacta
     2041 caactcgaac ccagaagacg aaaagcagtt gtgataaaag aatgtgtaga tatgtatgtg
     2101 cctaacccgc tcaaaaaaaa catgaaactc tactataaag aaactgagag tggaagccat
     2161 cgagttataa ttgacaacat tttgaaatta aagcagctga cgagaatttg tatgcgataa
     2221 atttttattg a
//
LOCUS       DAZFAB010000255         2214 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZFAB010000255 DAZFAB010000000
VERSION     DAZFAB010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.257"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..345)
                     /locus_tag="ACHJWA_004930"
     CDS             complement(<1..345)
                     /locus_tag="ACHJWA_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001626253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
                     /protein_id="HFO4311343.1"
                     /translation="MNNCIFFSISASAVTARQFQRELDLADKAILWALISASTKEGRK
                     ACSLSYFACKAAEAELGLAYMAANDNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGK
                     IIRPYLKNLNPLQ"
     gene            complement(980..1441)
                     /gene="yibG"
                     /locus_tag="ACHJWA_004931"
     CDS             complement(980..1441)
                     /gene="yibG"
                     /locus_tag="ACHJWA_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HFO4311344.1"
                     /translation="MKACLLLFFYFSFICQLHGADVKIKQNESMMGSTAMTYDLSEEK
                     LMKLKYKSQHGDSEASFRLYQYYCFTKNNIYKQLRYLEKSASQGNVTAQFNYGVFLSD
                     TNPTLSEYYNLNRAIYWMEFAVNNGNIDAKSKLQELKKLKRMDRRKNKENP"
     gene            complement(1453..>2214)
                     /locus_tag="ACHJWA_004932"
     CDS             complement(1453..>2214)
                     /locus_tag="ACHJWA_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO4311345.1"
                     /translation="LISAEGATEWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEES
                     GLYYNRHRYYDPLHGRYITQDPIGLKGGWNFYQYPLNPVINVDPQGLVDINLYPESDL
                     IHSVADEINIPGVFTIGGHGTPTSIESATRSIMTAKDIAYLIKFDGNYKDGMTVWLFS
                     CNTGKGQNSFASQLAKDLHTNVIGPDTLWTWWGRGTNGKLKMDTVLTAPTNLNSNKDL
                     MAITTKDLGNWITYGPSGHPISNMQGTPEKPSDIR"
BASE COUNT          625 a          474 c          385 g          730 t
ORIGIN      
        1 ttggagtgga tttagatttt tcagatatgg tcttatgatc tttcccttac ttaataaata
       61 gcatgtatag gattcggaaa gtccagcatc tattttgtat ctcattatat tagataagga
      121 tgtgagaaat tccttgttat cattcgcagc catatatgcc agtccgagtt cagcctctgc
      181 ggccttacag gcaaaatagc ttagtgaaca cgcttttcgt ccctctttag tactggctga
      241 tattagtgcc cataatattg ctttgtctgc caaatctaac tctctctgaa attgtcttgc
      301 agtaactgca ctcgcagaaa tagagaaaaa aatacaattg ttaataacct tgccatattt
      361 tttagtccgt aactgttaat gtgctatctg gctctgaaaa tattaactca tttaattttt
      421 gcatgtcatt agataaacca gttatacaac cctcggaaca gaatcgaggt cctaaagacg
      481 gaccaaaagc gtttaaacaa ctatgtattg aaaatccatt gcgattataa gttcctgtat
      541 taactgacgg tactaatcgt atgccgtcag gttttctatt gcttgctccc gggtcattaa
      601 cattccaact ccataggcct tgagatattg gccatctatt tataaatgtg tacaacgggg
      661 attattttta cattgcatat tattaccaga agcgactgga atactaagca catcataaaa
      721 attatcatca catggtatat ctgaaacaca cgaaagcata cctaaagtta tgctgtataa
      781 acaggtggct aatcccaagg ggatcgattc cagaaatcgg gttcaacgga tactgataaa
      841 aattccacct accttccagc ccaatcggag cctgagtgtt ataccgcccc tgctgcggtt
      901 aatgttaccg ataatagtta tagtacagcc ctgattcatc atcatactac tgctgcggca
      961 gtctgatgaa ctattgcttt tacggattct ccttattctt ccttctatcc attcgcttta
     1021 gctttttgag ctcttgaagt ttgctctttg catcaatatt accgttattt acagcgaatt
     1081 ccatccaata aatagctcta ttcaaattat aatattctga taatgttgga tttgtatctg
     1141 ataaaaagac cccataatta aactgcgctg taacattccc ctgagatgct gatttttcta
     1201 agtatcgcaa ttgtttgtaa atgttatttt tagtaaagca gtaatattga tatagtcgaa
     1261 atgatgcctc tgaatcacca tgttgtgatt tatacttcag tttcatcaac ttttcttcac
     1321 ttaaatcata ggtcattgct gtagacccca tcatactttc gttttgtttt attttcacat
     1381 cagcaccatg caattgacaa ataaaagaga aataaaaaaa tagtaacaag catgctttca
     1441 tacatctaca acctatctta tatcactggg tttttctggc gtaccttgca tattagaaat
     1501 ggggtgccca gatggcccat atgttatcca attaccaagg tcttttgttg ttatagccat
     1561 tagatcctta tttgaattaa ggttcgttgg tgctgttagc actgtatcca tttttaactt
     1621 accattagtt cctcgccccc accacgtcca tagcgtgtca ggtcctatta catttgtatg
     1681 taaatcttta gctaattggc tagcaaatga attttgtcct ttacctgtat tacaagaaaa
     1741 taaccaaact gtcatcccat ctttataatt cccatcaaat ttaattagat atgctatatc
     1801 tttagctgtc atgatactgc gcgttgcgga ttcaatagat gtgggggtac catgcccccc
     1861 gattgtgaaa acgcctggga tattaatctc atcagctaca gaatggataa gatcactttc
     1921 ggggtataaa tttatatcaa ccaaaccttg cggatctaca tttatgaccg gattcaacgg
     1981 atactgataa aaattccatc cccccttcag tccaatcgga tcctgagtga tatatcgccc
     2041 gtgcagcggg tcataatagc ggtggcggtt gtaatacagg ccggactcct catcatactg
     2101 ctgccccggc aggcggataa gctgctgcag ctgatgcggg ttctcttcat tcagcaggtt
     2161 gccccattca tcgtattctg cgcaccattc tgttgcccct tccgcactga taag
//
LOCUS       DAZFAB010000256         2205 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZFAB010000256 DAZFAB010000000
VERSION     DAZFAB010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.258"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            20..265
                     /locus_tag="ACHJWA_004933"
     CDS             20..265
                     /locus_tag="ACHJWA_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO4311346.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALKQKLRHNLEARLDAEVDA
                     ILDELLGAPAAPEPEGIAGEGSASDSGDPTPDSDMMM"
     gene            343..558
                     /locus_tag="ACHJWA_004934"
     CDS             343..558
                     /locus_tag="ACHJWA_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO4311347.1"
                     /translation="MNQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            562..1119
                     /locus_tag="ACHJWA_004935"
     CDS             562..1119
                     /locus_tag="ACHJWA_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311348.1"
                     /translation="MNHEEMNQRFSRLENEIAELNKKLSALMPSEDEKKRRDEQFAAF
                     DDYCRKVMSRNLAECFSIHNDNCSELEWECNRPSFVVSGDDGKITISENGKVTLPSHQ
                     HSETLIEFAIDYLKNNKKQGLMQRIGRCMGYLQVAAEIEALASGADKDAIVREALLRN
                     FNTPPFKKVPAYWLHPGLTYLKVRI"
     gene            1156..1689
                     /locus_tag="ACHJWA_004936"
     CDS             1156..1689
                     /locus_tag="ACHJWA_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO4311349.1"
                     /translation="MNTKIKYGLSAAVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVIPGMKLSKGKCDRVNAIERDKALAWVEKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            1988..>2205
                     /locus_tag="ACHJWA_004937"
     CDS             1988..>2205
                     /locus_tag="ACHJWA_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
                     /protein_id="HFO4311350.1"
                     /translation="MNRVLCVVIIALLVACGALSLGLNHYRDNAITYKAQRDKKAREL
                     ELANATITDMQVRQRDVAALDAKYSRELA"
BASE COUNT          572 a          471 c          644 g          518 t
ORIGIN      
        1 ctttcgacga gaaaatccca tgtcagaaat tacatccctg gtcactgctg aagcagtgaa
       61 ggaagtcctg cgctctgaag aagtccggag cgcactgaaa cagaaacttc gccataacct
      121 ggaagcgcgt cttgatgcag aagtggatgc cattctggat gaactgctgg gcgcaccggc
      181 agctccggag ccggaaggca tcgcgggtga ggggagtgct tcagacagcg gtgaccccac
      241 accggacagc gacatgatga tgtaagcatg cgtcagggac catcggtgtg tgccggtggt
      301 cttttttatt gttgtgagct tccggattgc gggaggcggg gtatgaacca gatggaaaaa
      361 atcacaacag gtgtgtcata caccacgtca gcggtgggaa cgggctactg gttcctgcag
      421 ttgctggaca gggtttcccc gtctcagtgg gcggcaatag gcgtgctggg gagtctgctg
      481 tttggtctgc tgacgtacct gacgaacctg tatttcaaaa tcagagagga caggcgtaag
      541 gcggcacggg gagagtaggt gatgaaccat gaagaaatga atcagcgctt cagtcgtctg
      601 gaaaatgaaa ttgctgaact gaataaaaaa ctgtcggcgc tgatgccttc tgaagatgaa
      661 aaaaaacgcc gcgatgagca gtttgctgcg tttgacgatt attgtcggaa agtgatgagc
      721 agaaatctcg cagagtgttt cagtattcat aatgataatt gcagtgagct ggaatgggag
      781 tgtaaccggc catcctttgt tgtatccggt gatgacggga aaatcaccat atcagaaaat
      841 ggaaaagtaa cactgccatc gcaccagcac agtgagacgc tcattgaatt tgccattgat
      901 tacctgaaga acaataaaaa acaggggctg atgcagcgca ttggtcgttg catggggtat
      961 ctgcaggtag ccgctgagat tgaagcgctg gccagtggtg ctgataagga tgcaattgtg
     1021 cgggaggctc ttcttcgtaa ttttaatact ccacccttta aaaaagtgcc ggcttactgg
     1081 cttcatcccg gactgactta tctgaaagtg cgtatttagt gggcctggga cagcggctga
     1141 atatttaata tatccatgaa cacaaaaatc aaatacggcc tgtcggctgc cgttctggcg
     1201 ctgattgccg caggtgcttc tgcgcctgaa attctcgacc agtttcttga cgaaaaagaa
     1261 ggtaaccaca ccacggcata ccgtgatggt gcgggtatct ggaccatctg ccgtggagcc
     1321 acccgggtgg atggtaagcc tgttattcct ggcatgaagc tgtcgaaggg gaaatgcgac
     1381 cgggttaacg ccattgagcg tgataaggcg ctggcatggg tggagaaaaa catcaaagtg
     1441 ccactgactg aaccccagaa agcggggatc gcgtcattct gcccgtataa cattggcccc
     1501 ggtaagtgtt tcccgtcgac gttttataaa cgaattaatg caggcgatcg caggggagcg
     1561 tgtgaagcga ttcgctggtg gattaaggac ggaggcagag actgccgtat ccgctcaaat
     1621 aattgctatg gtcaggtatc ccgtcgtgac caggagagcg cgctggcgtg ctggggtatc
     1681 gacagataag cagaatattt tgctgaaaaa tgacgttggc caacgcggac ggataacacg
     1741 aaatcctgcg aactggcaaa acctgagtga ataaaagtaa aaaccccgtt tgttagcagc
     1801 aggtggggtt ttgtgtttcc tgactccgga aaagtcaaag gagaaagtgt gtttgatttt
     1861 agcaaactga ttcgggagat ttgagtgatg gctgaaaaat tatctacctg gaagttcatt
     1921 cttatctggc tggtgtttgt gattatggtc tccggttatt tcatcggtca gatacgctgg
     1981 tggtgaaatg aaccgcgtac tgtgtgtggt catcattgcc ctgctggtgg cctgtggtgc
     2041 gcttagtctg gggctgaatc attaccgtga taacgccatc acctacaaag cgcagcgcga
     2101 taaaaaagcc agagagctgg agctggcaaa cgcaaccatt actgatatgc aggtgcgcca
     2161 gcgcgatgtt gctgcgctcg atgcaaaata ctcaagggaa ttagc
//
LOCUS       DAZFAB010000257         2169 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZFAB010000257 DAZFAB010000000
VERSION     DAZFAB010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.259"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..103
                     /locus_tag="ACHJWA_004938"
     CDS             <1..103
                     /locus_tag="ACHJWA_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311351.1"
                     /translation="AVKPLKFANQMYIDTGAVFCGNLTLIQVQGEGA"
     gene            100..723
                     /locus_tag="ACHJWA_004939"
     CDS             100..723
                     /locus_tag="ACHJWA_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO4311352.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAVFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTEQWGIVAEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAITR"
     gene            976..1719
                     /locus_tag="ACHJWA_004940"
     CDS             976..1719
                     /locus_tag="ACHJWA_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311353.1"
                     /translation="MLSSCIGVVMKDGALLRSSSLFIAYMGCLGWGSAYFYGWGTSFY
                     YGFPWWIVGAGVDDVARSLFFAVIVIAIFLIGWGIGVVFFFAVKRKHSMQELNVFRLY
                     FAVELLFVPAIIEFSILRQKIQVPLLLLSAAIALAVTISIRSYGRFLSVSCFYDKPFI
                     KKHFFEIVMIAFVAYFWLFSFLTGYYKPQFKKEYEMINYNDGWYYVLARYDNCLVLST
                     SFNAGSKRFVIYQSAQDKNLQVDIVRTRI"
     gene            1805..1972
                     /locus_tag="ACHJWA_004941"
     CDS             1805..1972
                     /locus_tag="ACHJWA_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFO4311354.1"
                     /translation="MFYKLCLLAVVILLLVMVMMDFTSRIMLVLTDGALVCGIVVLLW
                     PVIKRNSLHNA"
BASE COUNT          566 a          396 c          534 g          673 t
ORIGIN      
        1 agcagtgaaa ccactcaaat ttgccaacca gatgtatatc gatactggcg cagtgttctg
       61 cggaaacctc acattgattc aggtacaggg agaaggcgca tgagactcga aagcgtagct
      121 aaatttcatt cgccaaaaag cccgatgatg agcgactcac cacgggccac ggcttctgac
      181 tctctttccg gtactgatgt gatggctgct atggggatgg cgcaatcaca agccggattc
      241 ggaatggctg tattctgtgg taagcacgaa ctcagccaga acgacaaaca aaaggctatc
      301 aactatctga tgcaatttgc acacaaggta tcggggaaat accgtggcgt ggcaaagctt
      361 gaaggaaata ctaaggcaaa ggtactgcaa gtgctcgcaa cattcgctta tgcggattat
      421 tgccgtagtg ccgctacgcc gggcgcaaga tgcagagatt gccacggtac aggccgtgcg
      481 gttgatatag ccaaaacaga gcagtggggg atagttgctg agaaagagtg cggaagatgt
      541 aaaggcgtcg gttattcaag aatgccagca agcgccgcat atcgcgctgt gacgatgcta
      601 atcccaaacc ttacccaacc cacctggtca cgcactgtta agccgctgta tgacgctctg
      661 gttgtgcaat gccacaagga agagtcaatc gcagacaaca ttttgaatgc gatcacacgt
      721 tagcgccatg attgccacgg atggcaacat attaacggca taatattgac tttttgaata
      781 actttgggga aacttgacac caataatggg cgttttttac atgtcattga tgagtctcaa
      841 taacctgccg ccgagtagtt tttatgctct gaattgtatt tgtgtagtaa acatgctgac
      901 tgcaatgtaa tagagttttt ttagcctgta acctcttgac ggcattgaat tgcttttgtt
      961 atgagttgta agccaatgtt atcatcttgt attggggtgg ttatgaagga tggtgcgctg
     1021 ctcaggagtt cttcactttt tattgcctac atgggatgcc ttggatgggg gagtgcttat
     1081 ttctatggat ggggtacttc tttttactac ggcttcccat ggtggattgt aggtgcaggt
     1141 gttgatgatg ttgccagaag tttatttttt gcagttatcg tcattgctat atttcttatc
     1201 ggttggggta ttggtgttgt attctttttc gcagtgaaaa gaaaacattc tatgcaagag
     1261 ctaaatgtat ttcgccttta ttttgctgtg gaattattgt ttgtgccggc aattattgag
     1321 ttttctatat tgagacagaa gattcaggta cctcttttgc tactgtcagc agcgattgcg
     1381 ctggcggtta caatttcgat aagatcttat gggcgatttt tatcggtatc atgcttctat
     1441 gataagccat ttataaaaaa acattttttt gagattgtga tgattgcttt tgtggcatat
     1501 ttctggcttt tttcatttct gacaggatat tacaaaccac agtttaagaa agaatatgaa
     1561 atgattaatt ataatgatgg ttggtattat gttcttgctc gttatgataa ttgtctggtt
     1621 ttgtctactt ctttcaatgc aggtagtaaa aggtttgtga tttatcaatc agcacaagat
     1681 aagaatcttc aggttgatat tgtaaggacc agaatttaat tggctgcata aataatattt
     1741 taagttgcaa gttggctatt cgtaggaata gaaccttagg catgctgaat gcgtttcctg
     1801 aacattgttt tataaactgt gtctgcttgc tgttgtgatc ctgcttttag tgatggtgat
     1861 gatggatttc accagcagga taatgttggt actgactgat ggcgctctgg tctgcggcat
     1921 tgtggtattg ctgtggccgg tgataaaaag aaacagcctg cataatgctt gatttttttg
     1981 tttactgttt attaaaaata ctactgcatg gtgaatcccc ctgtgcggag gggcaatcag
     2041 caaccaggta tatgtgataa tcgcggattc aggtgctgat actgaattca ccgggaggca
     2101 cccggcacca tgcaagaaaa agaatgtgca tgcaaacatg cccctctccg gaggggcatt
     2161 ttttatggg
//
LOCUS       DAZFAB010000258         2126 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZFAB010000258 DAZFAB010000000
VERSION     DAZFAB010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.260"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..260)
                     /locus_tag="ACHJWA_004942"
     CDS             complement(<1..260)
                     /locus_tag="ACHJWA_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscC/YscC/HrcC family type III secretion system
                     outer membrane ring protein"
                     /protein_id="HFO4311355.1"
                     /translation="MKKISFFIFTALFCCSAQAAPSSLEKRLGKSEYFIITKSSPVRA
                     ILNDFAANYSIPVFISSSVNDDFSGEIKNEKPVKVLEKLSKL"
     gene            complement(260..715)
                     /gene="sepD"
                     /locus_tag="ACHJWA_004943"
     CDS             complement(260..715)
                     /gene="sepD"
                     /locus_tag="ACHJWA_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE switch protein
                     SepD"
                     /protein_id="HFO4311356.1"
                     /translation="MNNNNGIAKNDCDWLTALDFVKDVNGSPTHLTFYIYQKNAFLHD
                     FGNYWVLYIELSGDFRQVPTDTFIRLCNILAVSNEYKQMGIFLSNKKWYLCQIFHKDN
                     NHRANMSKAIMQHTLASLLDKQFDKLEQLSSSDTMMPPTHLFSDIGRIV"
     gene            complement(721..1293)
                     /gene="escJ"
                     /locus_tag="ACHJWA_004944"
     CDS             complement(721..1293)
                     /gene="escJ"
                     /locus_tag="ACHJWA_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscJ"
                     /protein_id="HFO4311357.1"
                     /translation="MKKHIKNLFLLAAICLTVACKEQLYTGLTEKEANQMQALLLSND
                     VNVSKEMDKSGNMTLSVEKEDFVRAITILNNNGFPKKKFADIEVIFPPSQLVASPSQE
                     NAKINYLKEQDIERLLSKIPGVIDCSVSLNVNNNESQPSSAAVLVISSPEVNLAPSVI
                     QIKNLVKNSVDDLKLENISVVIKSSSGQDG"
     gene            complement(1296..1673)
                     /gene="sctI"
                     /locus_tag="ACHJWA_004945"
     CDS             complement(1296..1673)
                     /gene="sctI"
                     /locus_tag="ACHJWA_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HFO4311358.1"
                     /translation="MNNINQSENINIQLNKAPQTNFVDEHTSLASAPSAAGAAQFLDQ
                     LLPKTAGVSSPEQVLIEEIKKRHLATMNSDLSFDALSAGGLSPEDVLTLQKNVLNANV
                     NVDVVSKLASLLSTSVTKLVSMQ"
     gene            complement(1757..2056)
                     /gene="espZ"
                     /locus_tag="ACHJWA_004946"
                     /gene_synonym="sepZ"
     CDS             complement(1757..2056)
                     /gene="espZ"
                     /locus_tag="ACHJWA_004946"
                     /gene_synonym="sepZ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE cytoprotective
                     effector EspZ"
                     /protein_id="HFO4311359.1"
                     /translation="MEAANLSPSGAVMPLATSLSGNNSVDEKTGVIKPENGTNRTVRV
                     IAGLALTTTALAALGTGIAAACSETSSTEYLALGITSGVLGTLTAVGGALAMKYA"
BASE COUNT          591 a          413 c          412 g          710 t
ORIGIN      
        1 tatagtttgg acagtttttc cagtactttt acaggttttt cattttttat ttcgccagag
       61 aaatcatcat tcacggaact actgatgaaa acaggaatgc tgtagttagc agcaaagtca
      121 ttaaggattg cacgtactgg agaactttta gtaataataa aatattcgct cttgcctaat
      181 cttttttcca gagaggacgg ggcagcttgt gcactgcagc aaaatagtgc tgtaaaaata
      241 aaaaaactta tttttttcat tacacaattc gtcctatatc agaaaacaag tgtgttggcg
      301 gcatcattgt atccgatgaa gataattgct ctagtttatc aaattgctta tcgagcaaag
      361 aggcgagagt atgttgcatt attgcttttg acatgtttgc gcgatgatta ttatctttat
      421 gaaaaatttg gcaaagatac cacttcttat tagacaggaa aatacccatt tgtttatatt
      481 cgttactaac ggccaggata ttgcagagtc tgataaatgt atctgttgga acctgacgaa
      541 aatcaccact aagctcaata tatagaaccc agtagtttcc aaaatcgtgc aagaaagcat
      601 ttttttggta gatataaaaa gtgagatgcg ttggtgaacc gttgacatcc tttacaaaat
      661 caagggccgt tagccagtca caatcattct ttgctatgcc attattattg ttcatacata
      721 ttacccgtcc tgtcctgagg atgacttgat aacaacacta atattttcta atttgagatc
      781 gtcaacacta ttcttaacta ggttttttat ctgaatgacc gatggtgcta agttaacctc
      841 tggcgagctg atgactaaaa cggctgctga ggagggttga ctttcgttat tgttaacatt
      901 taatgaaacg ctgcaatcga taacaccagg tattttactt agcaaccttt cgatgtcttg
      961 ttctttgaga tagttgattt ttgcattttc ttgtgatggc gatgccacta actggctagg
     1021 tgggaaaata acctcgatat ctgcaaattt ctttttcggg aagccgttat tatttaaaat
     1081 cgttattgcg cgaacaaaat cctctttttc cacactgagt gtcatatttc cacttttgtc
     1141 catttctttg gaaacattga catcattgga tagcaacagt gcttgcatct gattagcttc
     1201 tttttctgtt aatcctgtat acaattgctc tttacatgcc actgtcaaac aaatagcagc
     1261 caataaaaaa aggtttttaa tgtgtttttt catttttatt gcatcgaaac taattttgta
     1321 accgatgttg aaaggaggct tgctaactta gatacgacat caacgttgac gtttgcgttg
     1381 agcacattct tttgtaaggt gagcacgtct tctggcgaga gcccacctgc agatagagca
     1441 tcgaaactga gatcgctgtt cattgttgca agatgtctct ttttaatttc ttcaatcaac
     1501 acttgttctg gagaagacac tcccgctgtt ttagggagta attgatcaag aaactgagcc
     1561 gcacctgccg cagagggggc tgaggctaac gaggtatgct catcaacgaa atttgtttgg
     1621 ggcgctttat ttaattgaat attaatgttt tccgattgat taatgttgtt catatttact
     1681 gttagcctct ttttatgaaa gaggcaaggg cataatacat ccattacctc tatcttttat
     1741 ttatgcgatg ggccggttag gcatatttca tcgctaatgc accgccaacc gcagtaagag
     1801 tacctagtac gccagaagta atacccaggg ctaagtattc tgtgctgctc gtctccgagc
     1861 atgccgctgc aatacctgta cctagagctg ccagagccgt agtggtaagt gctaatccgg
     1921 ctataactct aacggtgcga tttgttccat tttctggttt aatcactcct gtcttctcat
     1981 ccactgagtt atttccactg agtgaggtcg ccagcggcat tactgcacca gaaggactta
     2041 aatttgctgc ttccattgat ctttctcctt ttgtctaatt tagacattta cctggttata
     2101 aataaccaaa ttatcccata ggcaac
//
LOCUS       DAZFAB010000259         2005 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZFAB010000259 DAZFAB010000000
VERSION     DAZFAB010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.261"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            1..186
                     /locus_tag="ACHJWA_004947"
     CDS             1..186
                     /locus_tag="ACHJWA_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO4311360.1"
                     /translation="MNTAAEAIRALSLQMPGFRRQMNEGWYQTRIPTASQNRSPAPVF
                     VVMSGEYLLDKKEEIIQ"
     gene            199..288
                     /locus_tag="ACHJWA_004948"
     CDS             199..288
                     /locus_tag="ACHJWA_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311361.1"
                     /translation="MPINLTSYLWVQGQKVVPAVVFSNFTLVD"
     gene            308..>2005
                     /gene="nleL"
                     /locus_tag="ACHJWA_004949"
     CDS             308..>2005
                     /gene="nleL"
                     /locus_tag="ACHJWA_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector E3 ubiquitin
                     ligase NleL"
                     /protein_id="HFO4311362.1"
                     /translation="MLPTTNISVNSGVISFESPVDSPSNEDVEVALEKWCAEGEFSEN
                     RHEVASKILDVISTNGETLSISEPITTLPDLLPGSLKELVLNGCTELKSINCLPPNLS
                     SLSMVGCSSLEVINCSIPENVINLSLCHCSSLKHIEGSFPEALRNSVYLNGCNSLNES
                     QCQFLAYDVSQGRACLSKAELTADLIWLSANRTGEESAEELNYSGCDLSGLSLVGLNL
                     SSVNFSGAVLDDTDLRMSDLSQAVLENCSFKNSILNECNFCYANLSNCIIRALFENSN
                     FSNSNLKNASFKGSSYIQYPPILNEADLTGAIIIPGMVLSGAILGDVKELFSEKSNTI
                     NLGGCYIDLSDIQENILSVLDNYTKSNKSILLTMNTSDDKYNHDKVRAAEELIKKISL
                     DELAAFRPYVKMSLADSFSIHPYLNNANIQQWLEPICDDFFDTIMSWFNNSIMMYMEN
                     GSLLQAGMYFERHPGAMVSYNSSFIQIVMNGSRRDGMQERFRELYEVYLKNEKVYPVT
                     QQSDFGLCDGSGKPDWDDDSDLAYNWVLLSSQDDGMAMMCSLSHMVDMLSPNTSTNWM
                     "
BASE COUNT          607 a          317 c          430 g          651 t
ORIGIN      
        1 gtgaacacgg cagcggaagc catccgtgcc ctgtcgctgc agatgccggg attccgccgt
       61 cagatgaacg aaggctggta ccagacacgt attccgacag cttcacaaaa ccggagtccg
      121 gctccggttt ttgttgtcat gtccggggaa tatttgttag ataaaaaaga ggagataatt
      181 caatagggag ttaaattaat gccgataaat ctgacatctt atttgtgggt acagggacag
      241 aaagttgtcc cggcagttgt gttttctaat tttactttag tggactaagt aaaaaggagt
      301 gagataaatg ctgcccacta caaatatctc tgtaaattct ggagtaatat cttttgaaag
      361 tcctgtagat tcaccatcta acgaggatgt tgaagttgcc ctcgaaaagt ggtgtgctga
      421 gggagaattt agcgaaaatc gtcatgaggt tgcatcaaaa atacttgatg ttataagtac
      481 taatggagag actttatcaa tcagtgagcc aataacaaca ttaccagact tgcttccagg
      541 ttctctgaaa gaactggttt tgaatggatg tacagagctt aaatcaataa actgcttacc
      601 ccccaactta tcttcattaa gtatggttgg atgctcatca ttagaggtta taaattgcag
      661 catacctgaa aatgtcatta atttatcttt atgccattgt agttctttga aacatataga
      721 aggttccttt cctgaggcac tcagaaattc cgtatattta aatggctgta attcattaaa
      781 tgaatcgcaa tgtcaattcc ttgcatatga tgtcagtcaa ggccgtgcct gcctgagcaa
      841 agctgagctt actgctgact taatttggtt gtcagctaac cgaacgggtg aagagtctgc
      901 tgaagaattg aattactctg gatgtgactt gtcaggtcta agtcttgtag ggctgaattt
      961 atcatcagta aatttttctg gagcagtgct tgatgataca gatctcagga tgagtgattt
     1021 gtctcaggct gtattggaaa actgttcttt taaaaactcg attttgaatg aatgtaattt
     1081 ttgttatgct aatttatcta attgtattat tagggctttg tttgaaaact ctaatttcag
     1141 caattccaat cttaaaaatg catcatttaa aggatcttca tatatacaat atcctccaat
     1201 tttgaacgag gctgatttaa caggagctat tataattcct ggaatggttt taagtggtgc
     1261 tatcttaggt gatgtaaagg agctctttag tgaaaaaagt aataccatta atctaggagg
     1321 gtgttacata gatctatctg acatacagga aaatatatta tctgtgttgg ataactatac
     1381 aaaatcaaat aaatcaattt tattgactat gaatacatct gatgataagt ataaccatga
     1441 taaagtaagg gccgctgaag aacttatcaa aaaaatatct cttgacgaat tagcggcgtt
     1501 ccggccctat gttaagatgt ctttggctga ttcatttagt attcatcctt atttgaacaa
     1561 cgcaaatata cagcaatggc tcgagcctat atgtgatgac ttttttgata ctataatgtc
     1621 ttggtttaat aattcaataa tgatgtatat ggagaatggt agtttattgc aggcagggat
     1681 gtattttgag cgacatccag gtgcgatggt atcttataat agttccttta tacaaattgt
     1741 aatgaatggt tcacggcgtg atggaatgca ggaacgattt agggaactct atgaagtata
     1801 tttaaaaaat gaaaaagttt atcctgtcac acagcagagt gattttggat tgtgcgatgg
     1861 ctctgggaag cctgactggg atgatgattc cgatttggct tataactggg ttttgttatc
     1921 atcacaggat gatggtatgg caatgatgtg ttctttgagt catatggttg atatgttatc
     1981 tcctaataca tcaactaatt ggatg
//
LOCUS       DAZFAB010000260         1987 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZFAB010000260 DAZFAB010000000
VERSION     DAZFAB010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.262"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(495..1022)
                     /locus_tag="ACHJWA_004950"
     CDS             complement(495..1022)
                     /locus_tag="ACHJWA_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HFO4311363.1"
                     /translation="MDNSKHSDEAEKLLAELSARKGEGEGEGEGEGEPKSTVSVFYLQ
                     PEEVNTLSHQAKRGDGEAGFRLFLYYKLSNYDEEKSSQWLKIAANNGHATAQYRLYVE
                     LQEQGETKEANEWLQKIKLAASNNDSSAIWFLVNCYKNQGNKKEALQWAYKLKASKQP
                     LDADRLIEELTKEQK"
     gene            complement(1091..>1987)
                     /locus_tag="ACHJWA_004951"
     CDS             complement(1091..>1987)
                     /locus_tag="ACHJWA_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO4311364.1"
                     /translation="RLERELRQGSVSEESQQWLAQCGLTAEQMAAQLEAEYIPERKLH
                     LYHCDHRGLPLALISPEGETAWQGEYDEWGNLLGETSAQHLQQSLRLPGQQYDEESGL
                     YYNRNRYYDPLQGRYITQDPIGLEGGWNLYQYPLNPIEHIDPLGLALDLNYYSPSDPI
                     YKGSLNVREFPTGFTVGGHGSPTSMSDDRIKKGSDLTIKQLASDIRANPKYHEGMPVV
                     LFSCETGKGKNSFAQKLANELDATVIAPDEIIWIWPDGNYAIMGQTARITIGGKDNGV
                     FELVPDEKQPGDFHKFTPTGSK"
BASE COUNT          510 a          493 c          350 g          634 t
ORIGIN      
        1 taacaaaaaa cagaacacta tcaaaacata aaaagcaaac aaaaaacaat aagcatgttg
       61 attgaatatt ttaaatacat gttcttttac aataaaaaaa tgaatggtta tcgaacaagc
      121 cagcaataat aaccttatta ttttcataag ctccatccac taaaatgctg gagatataaa
      181 aaatgaacca aacggcttct ttggcccata cttaggtttg gggtgtagaa tcttagtcat
      241 aagatctatg ccgagtcatg tgctggccac atacttaatg atacaaaaag ctatattttc
      301 atcttttctc ttgcgacttt tgatgcatct gccaagtaca tcgtaatgat aaccggcgac
      361 gaagatgcca gttttggttt caacttgcgc tttgatgagc ttgttgcagt cgtcgtaaac
      421 gtaattttgt gtgtaggtgt tgtagcgact ggtaaagttt ttctactctc catttttctg
      481 ttacctgttt aaagctactt ttgttctttc gttaattctt caattagtcg atctgcatca
      541 agcggctgtt tactcgcttt gagtttatat gcccattgca atgcttcttt tttatttccc
      601 tggtttttat aacaattcac caagaaccat attgctgaac tgtcattatt actggcagcc
      661 aatttaattt tttgtaacca ttcattagct tccttggttt caccttgttc ttgtaactct
      721 acatataacc gatactgagc tgtcgcatga ccattattag ccgctatttt taaccactga
      781 ctactttttt cttcatcata attggacaac ttgtagtaca agaacaacct aaaacccgcc
      841 tcgccatcgc ctcgttttgc ctggtgtgat aaagtattta cttcttctgg ctgcaaataa
      901 aagacgctta ctgttgattt tggttcacct tcaccttcac cttcaccttc accttcacct
      961 tttctagcag aaagctccgc caataatttt tccgcctcat cagaatgttt tgagttatcc
     1021 atagcagccc tcactccaaa gttataaaga cataccatga taatcaacaa aaaagtaaat
     1081 actttcatta ctatttgctt cctgtaggtg taaacttatg aaagtcccct ggttgtttct
     1141 cgtctggcac caattcaaac accccgttgt ccttaccgcc tattgttatc ctagctgttt
     1201 gccccataat agcatagttt ccatccggcc aaatccatat tatttcatca ggagcaatca
     1261 ctgtagcatc aagttcgtta gctagtttct gtgcaaatga atttttgcct tttcctgttt
     1321 cgcaggaaaa caagactaca ggcatacctt catgatattt aggattcgct cttatgtcgc
     1381 tagctaattg ttttattgtc agatcacttc cttttttgat tctgtcatca ctcatagatg
     1441 taggcgaacc atgacctcca actgtaaaac ccgttggaaa ttcccttaca ttaagagaac
     1501 ctttatatat aggatctgac ggagaataat aattcaaatc aagtgctaac cccaacggat
     1561 ctatatgttc aataggattg agtgggtact gatacaggtt ccatcccccc tccagcccta
     1621 tcgggtcctg ggtgatatat ctcccctgca acggatcata gtaccggttg cggttgtagt
     1681 acagccccga ctcctcatca tactgctgcc ccggcagacg gagtgactgt tgaaggtgct
     1741 gcgcgctggt ttcgcccagc aggtttcccc actcgtcata ctccccctgc cacgccgttt
     1801 ccccttccgg gctgatgagc gccagcggca gtccccggtg gtcgcagtgg taaagatgaa
     1861 gtttcctctc cgggatgtat tccgcctcca gctgcgcggc catctgctcc gccgtcagcc
     1921 cgcactgcgc aagccactgc tggctttctt cactcacgct gccctgccgc agctcccgct
     1981 ccagcct
//
LOCUS       DAZFAB010000261         1978 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZFAB010000261 DAZFAB010000000
VERSION     DAZFAB010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.263"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            31..165
                     /locus_tag="ACHJWA_004952"
                     /pseudo
     CDS             31..165
                     /locus_tag="ACHJWA_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309987.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(269..339)
                     /locus_tag="ACHJWA_004953"
     tRNA            complement(269..339)
                     /locus_tag="ACHJWA_004953"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(309..311),aa:Met,seq:cat)
     gene            complement(385..1443)
                     /locus_tag="ACHJWA_004954"
     CDS             complement(385..1443)
                     /locus_tag="ACHJWA_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO4311365.1"
                     /translation="MFNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWTGDEDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYEAKGRALKQHVMA
                     PLISYFRDARAALGITAKQIVDATGKKNMVSHWFSASQWQLPNESDYLKLQALFARVA
                     EEKHQRGELEKPHHQLLETYTSLNRQYAELQSEYKHLRRYFGVTAQVPYTDVWTHKPV
                     QYYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAAMALGRRATGVELET
                     ERFEQTVREVQDLVSQNG"
     gene            complement(1594..1791)
                     /locus_tag="ACHJWA_004955"
     CDS             complement(1594..1791)
                     /locus_tag="ACHJWA_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO4311366.1"
                     /translation="MLKQQDMTETARAVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
BASE COUNT          470 a          577 c          452 g          479 t
ORIGIN      
        1 acccataaaa aagcccctcc ggagaggggc atgtttgcat gcacattctt tttcttgcat
       61 ggtgccgggt gcctcccggt gaattcagta ccagcacctg aatccgcgat taccccatat
      121 acctggttgc tgattgcccc tccgcacagg gggattcacc atgccagttt cttttaacaa
      181 actccccgca aaccagacaa cagtcaaccg cctgaattgt gagacattta aaaaaaggcc
      241 cgcaaaagcg agccagggaa aataagtgtg gcgcgttgta ctggattcga accagcgacc
      301 tggcgattat gcgtcgctcg ctctcaccac tgagctaaag ggccgggcgc aggataataa
      361 cggtacgtaa ctaatcctgc aatatcatcc gttctgactg actaaatcct gtacttccct
      421 gaccgtctgc tcaaaacgtt cagtctccag ctcaacgcca gttgcacgac gccccagcgc
      481 catcgcggct ttgactgtcg aacccgaccc catgaagaaa tctgcaacca ggtcacccgg
      541 acgactgctc gcactgatta tctgctgcag catttctgcc ggtttttcgc acggatgttt
      601 cccgggatag tactgcaccg gtttatgcgt ccacacatcc gtgtacggca cctgcgccgt
      661 cacgccaaaa taccgccgca gatgtttata ttcactctgc agttccgcat actgccggtt
      721 cagtgaagta tacgtctcca gcagctggtg gtggggcttt tccagttcac cgcgctgatg
      781 cttctcttct gccacccggg caaacagcgc ctgtaatttc agataatcgc tttcgttcgg
      841 tagctgccac tgactggcac tgaaccagtg cgacaccatg tttttctttc ctgtggcatc
      901 cactatctgt tttgccgtta tccccagggc agcacgcgca tcacgaaagt aagaaatcag
      961 cggagccatc acatgctgtt tcagtgccct gcccttcgcc tcatacccgg catctttcgg
     1021 acgatacggc ccctgataat gttccgcgaa cagaatgcgc tctgtggcgg ggaaatacgc
     1081 cctcaggctt tccttgttgc acccgttcca gcgtccggac ggcttcgccc agataatatg
     1141 gttcagcaca ctgaagcgtt cacgcatcat gatttcaatg tcagatgcca ggcgatggcc
     1201 acagaacagg taaagacttc cggcaggttt cagcacccgc cagaactgcg ccagacactg
     1261 gtccagccat ttcaggtaat cctcatcacc cgtccactgg ttatcccagc cctcaggctt
     1321 cactttaaag tacggcgggt ccgtgactat caggtcaaca gaattttcgg gtaacgaccg
     1381 gataaattcc aggcagtcgg cgttgattaa ctcacaactg gatattttta cagtattaaa
     1441 catggatcat taagcctgtc tctgataggc tcataccgct tttgcgcaaa gcagtgggcc
     1501 tgaggtttgc ttgtgatcca gacgcatgag cagatggctg gtgagtgccc ctaacaccca
     1561 ccagtcgccc atttaccaca aataaaaaag ccttcaggac tgaaggcgtc tgtaacaacc
     1621 gaactgatag tctgccagac ccgccataac aagctgggtc agtattaact ggcagcgttc
     1681 gcgtgaaagg taagtattct gcgcaatctc cccgactgtc gccggttcgg tgacgcttaa
     1741 ttcattaaac actgctctgg cagtttctgt catatcctgc tgtttcagca tgtctttttc
     1801 cctttttcgg ttaacgtgac acaccaataa ctcttgtcaa aaaagccagc aagctgaaag
     1861 accggtattc accgccacca gcgcgtttac tgtactgacg cgatttcagt cataaaaaac
     1921 ccgccaggcg gcggggtgta aaaaatcttc taacgtcagg cataaaacgc ccatcgtt
//
LOCUS       DAZFAB010000262         1899 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZFAB010000262 DAZFAB010000000
VERSION     DAZFAB010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.265"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(218..385)
                     /locus_tag="ACHJWA_004956"
     CDS             complement(218..385)
                     /locus_tag="ACHJWA_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFO4311367.1"
                     /translation="MTVKLRLAVAALLLFLVVMVDFTSRIMSVLADGVLVCGIVVLLW
                     PVIKRNSLHNA"
     gene            complement(382..813)
                     /locus_tag="ACHJWA_004957"
     CDS             complement(382..813)
                     /locus_tag="ACHJWA_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance protein"
                     /protein_id="HFO4311368.1"
                     /translation="MFGIFKKKTRRAATEIKKFEKRDLAQVVINAAYLVACADGECEA
                     SEKAKIEQVLRNQPALSAFTSEINAISATIIGQLDTNFKTGRRAALREIEDVKHDTRE
                     AEDVLDVAVAIAEADGEIEPEERKVLEEIAGVLGLRLENHL"
     gene            complement(983..1059)
                     /locus_tag="ACHJWA_004958"
     tRNA            complement(983..1059)
                     /locus_tag="ACHJWA_004958"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1023..1025),aa:Arg,seq:tct)
     gene            complement(1160..1235)
                     /locus_tag="ACHJWA_004959"
     tRNA            complement(1160..1235)
                     /locus_tag="ACHJWA_004959"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1200..1202),aa:Ile,seq:cat)
     gene            complement(1264..>1899)
                     /locus_tag="ACHJWA_004960"
     CDS             complement(1264..>1899)
                     /locus_tag="ACHJWA_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4311369.1"
                     /translation="PGSVVLATKCIRELFLMHYGKVKIVDISESVVSQYLESQHKLTR
                     TRLTDIPLYLLLEPNNPALAAALITSQGFSGEVTDMFLMMACLSLFETDERMSLFLSG
                     CLSSISAKVRAIIQTDISASWTLGAIALQLHMSESLLKTKLKNEGGMFSRLLLEERMR
                     VAVNMLCSRYGYGQAVAEKCGYSSRSYFISVFHRYYGFPPDRYVSRQGLDY"
BASE COUNT          505 a          527 c          420 g          447 t
ORIGIN      
        1 cgcgccatgc gcagccggaa ataaagggat aacgatgata gtttgagaaa aacagaaaca
       61 acacttttgt ggcaaagcat ggtgccgggt gcctcccggt gaattcagta tcagcacctg
      121 aatccgcgat tatcccatat acctggttgc tgatcgcccc tccgcacagg gggattcacc
      181 atgcagaagt gtttttaata aacagcaaac aaaaaaatca agcattatgc aggctgtttc
      241 tttttatcac cggccacagc aataccacaa tgccgcagac cagcacccca tccgccagca
      301 ccgacatgat tctgctggtg aaatccacca tcaccaccag aaacagcagg agtgcagcca
      361 cagccaggcg cagttttacc gtcacaggtg attctccaga cgaagaccca gaacaccggc
      421 aatctcttcc agcaccttgc gctcttccgg ctcaatttcg ccgtctgcct ccgcaatggc
      481 caccgccaca tccagcacat cttccgcttc acgcgtatcg tgtttcacat cctcaatctc
      541 acgtaacgcc gcacgacgac cagttttaaa gttcgtatcc agctgaccga taatggttgc
      601 gctaatcgca ttaatttctg acgtaaacgc ggacagcgca ggctgattac gcagtacctg
      661 ttcgatcttc gctttctcgg atgcctcaca ttcaccatct gcacaggcca ccaggtatgc
      721 ggcgttaatc accacctgtg ccagatcgcg tttttcaaac tttttaattt ccgttgccgc
      781 tctgcgggtt ttctttttga agattccaaa catcgtgacg ttcctttggg tgggtgagcc
      841 aacgcccggg agcgatctgc ccacagagaa agtcacactg accactccgt aagctcaccc
      901 ccgaaaggct ctgtggttgg tatgcgccgg gcgtggcgca gatacaaaaa aggcccgccg
      961 aagcgagcct ggaaaataag tgtggcgcgt tgtagtggag tcgaaccact gaccgattgc
     1021 ttagaaggca attgctctgt ccggctgagc taacaacgca gaataccgat aatggaccgc
     1081 catcggggac ccggccccgc accaacaacc ctgttatggt gtcgtctgct cttcctgata
     1141 agctaatggc ggtttgtgat ggtggccctt gctggatttg aaccagcgac ctggcgatta
     1201 tgagtcgctc gctctcacca ctgagctaaa gggccgagcc aaaaaataat aatcagatga
     1261 aaatcaataa tcaagccctt gcctggatac atatctgtct ggcgggaagc cataatagcg
     1321 gtgaaataca gaaataaagt aggaccggct tgaataacca catttttctg ctacagcctg
     1381 tccatatcca taccgggaac ataacatatt tacagcaaca cgcatccgct cttccagcaa
     1441 caagcgactg aacatgcccc cttcattttt cagttttgtc tttaacaaac tctcactcat
     1501 atgcaactgt agagcaatcg caccaagcgt ccagcttgct gatatatctg tctgaattat
     1561 cgccctgact ttggcactta tgctggataa acatccactt aaaaacaatg acatccgttc
     1621 atctgtttca aacagagaca ggcaggccat cataagaaac atatccgtga cctctccgga
     1681 aaatccctgg ctggtaatta aagccgcagc caacgcagga ttgttgggtt ccagcaacag
     1741 gtaaagcgga atgtcagtca gacgagtcct cgtcagctta tgctgacttt ccagatattg
     1801 acttacgacg gattcgctta tatcgacaat tttaactttg ccataatgca taaggaaaag
     1861 ctccctgatg catttggtgg ccagaacgac tgagcctgg
//
LOCUS       DAZFAB010000263         1878 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZFAB010000263 DAZFAB010000000
VERSION     DAZFAB010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.266"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..507
                     /locus_tag="ACHJWA_004961"
     CDS             <1..507
                     /locus_tag="ACHJWA_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO4311370.1"
                     /translation="DKGERGDTGPVGATGERGPRGDTGPAGPQGPKGDRGERGETGLT
                     GNAGPQGPKGDTGAAGPAGPQGPKGETGAAGPVGATGPQGPKGDPGETQIRFRLGPAS
                     IIETNSHGWFPDTDGALITGLTFLDPKDATQVQGLFRHLQVRFGDGPWQDVKGLDEVG
                     SDTGRTGE"
     gene            509..778
                     /locus_tag="ACHJWA_004962"
     CDS             509..778
                     /locus_tag="ACHJWA_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO4311371.1"
                     /translation="MNILKKLMQRLCGCGKHDDRENGELLTAQLRLGPADILESDENG
                     IIPEQARVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
     gene            1038..1735
                     /locus_tag="ACHJWA_004963"
     CDS             join(1038..1287,1287..1735)
                     /locus_tag="ACHJWA_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFO4311372.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSPCRKTWQLQFT
                     YTASQPGTHQKIIDMAMNGVGCRASARIMGVGLNTVLRHFKKLRPQSVTSRIQPGSDV
                     IVCAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVLGERTLATLERLLSLLSAFEV
                     VVWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARPGRKSLSFSKSVELH
                     DKDIGHYLNIKHYQ"
BASE COUNT          472 a          427 c          565 g          414 t
ORIGIN      
        1 gataaaggag agcgtggaga caccggccct gtcggggcaa ccggtgaacg gggaccgaga
       61 ggagatacag gtccggcagg cccgcagggg ccgaaaggtg acaggggaga gcggggagag
      121 accggtctga cgggaaatgc aggtccacag gggccaaagg gagataccgg tgcggcaggc
      181 ccggcaggcc cacagggacc gaaaggagaa acaggtgcgg ctggcccggt gggggcaacc
      241 ggacctcagg gaccgaaggg cgacccgggg gagacacaaa tccgttttcg tctggggccg
      301 gcgagcatta ttgagacaaa cagccatggc tggttcccgg atacagatgg tgcgctcatc
      361 accggactga cctttcttga ccccaaagat gccacacagg ttcaggggct gtttcggcat
      421 ttgcaggtca ggtttggtga cgggccgtgg caggatgtta aggggctgga tgaagtgggc
      481 agtgatacag gcagaacagg agaatgacat gaatatacta aaaaaactta tgcagcgtct
      541 gtgcggttgc ggaaagcatg atgaccgtga aaacggggag ttacttacag cacagctgcg
      601 actgggaccg gcagacattc tggagtccga tgagaatggt attattccgg agcaggccag
      661 ggtaatcacg caggtggtga tactggatgc ggataaaaag cagatacagt gcgtggtaag
      721 accgctgcaa atcctgcgtg ctgacgggac gtgggaaaat attggcggga tgaaatagcc
      781 gacagcttca caaaaaccgg agtccggctc cggtttttgt tgtcatgtcc ggtggatgtt
      841 tgttaggaat gttcagacag gtttattttg aatttacaca gaatcctaaa caggttcgag
      901 aattaagaaa gaggttgtat gtttagcata agaaccctac tacctattag cgccagcgta
      961 tcagttccga caaaacaatc tcggtaatgc tgccaactta ctgatttagt gtatgatggt
     1021 gattttaagg tgcttgcgtg gcttccattt ccatcagatg tccttcctgc tccgctactg
     1081 aaggcgtggt gcgtaacggc aaaagcactg ccggacatca gcgctatctc tgctctcctt
     1141 gccgtaaaac atggcaacta cagttcactt acaccgcctc tcagcccggt acgcaccaga
     1201 aaatcattga tatggccatg aatggcgtcg gatgtcgcgc cagtgcacgc attatgggcg
     1261 ttggcctcaa cacggtttta cgtcacttaa aaaactcagg ccgcagtcgg taacctcgcg
     1321 catacaaccg ggcagtgatg tgattgtctg cgctgaaatg gacgaacagt ggggctacgt
     1381 cggtgctaaa tcacgtcagc gctggctgtt ttacgcgtat gacaggatac ggaggacggt
     1441 tgtggcgcac gtcctcggtg aacgcactct ggccacactg gagcgtcttc tgagcctgct
     1501 gtcggccttt gaggtcgtgg tatggatgac ggatggctgg ccgctgtatg aatcacgcct
     1561 gaagggaaag ctgcacgtta tcagcaagcg ttacactcag cgcattgagc gacataatct
     1621 gaatctgaga caacacctgg caaggccggg acggaagtca ctgtcgttct caaaatcggt
     1681 ggagctgcat gacaaggaca tcgggcatta tctgaacata aaacactatc agtaagttgg
     1741 agtcattacc taaaggcctc tctacactgt gcttgtatgg ggcttccctg atcatggcgg
     1801 ttatctatgt aatagcatat gtacttacgt ggtttctgag gtggtagcgc gtaatattat
     1861 tttatgtaaa aatatctt
//
LOCUS       DAZFAB010000264         1864 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZFAB010000264 DAZFAB010000000
VERSION     DAZFAB010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.267"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..131)
                     /gene="ydcD"
                     /locus_tag="ACHJWA_004964"
     CDS             complement(<1..131)
                     /gene="ydcD"
                     /locus_tag="ACHJWA_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdcD"
                     /protein_id="HFO4311373.1"
                     /translation="MLQIIRGKLVIFLITLCLFVVYLGFDNNSNSDIVFYGHKTPKSV
                     "
     gene            complement(115..>1864)
                     /locus_tag="ACHJWA_004965"
     CDS             complement(115..>1864)
                     /locus_tag="ACHJWA_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO4311374.1"
                     /translation="HRETARSFGGAGSTAGYEQVTAYTLTGQLQSRHLNLPQLDCDYD
                     WNDNGQLIRISGPQESREYRYSDTGRLTGVHTTAANLDIDIPYATDPAGNRLPDPELH
                     PDSTLTAWPDNRIAEDAHYVYRYDEYGRLTEKTDRIPEGVIRMHDERTHHYHYDNQHR
                     LVFYTRIQYGEPLVESRYLYDPLGRRTGKRVWRRERDLTGWMSLSRKPEVTWYGWDGD
                     RLTTVQTGTTRIQTVYRPGSFTPLIRIETENGEREKAQRRSLAEKLQQEGSEDGHGVV
                     FPAELVRMLDRLEEEIRADRVSSESRAWLAQCGLTVEQLEKQVEPEYTPARTLHLYHC
                     DHRGLPLALISEDGNTAWSAEYDEWGNQLNEENPHHLHQPYRLPGQQHDEESGLYYNR
                     HRYYDPLQGRYITPDPIGLRGGWNMYQYPLNPIQVIDPMGLDAIENMTSGGLIYAVSG
                     VPGLIVANSITNSAYQFGYDMDAIVGGAHNGAADAMRYCYLMCRMTKTFGSTIADVIG
                     KNHEAAGDRQGQPAKERIMDLKNNTVGIACGDFSAKCSDACIEKYNIGQLFGLDGIKA
                     DNPIKAKQGSSDASNY"
BASE COUNT          356 a          572 c          454 g          482 t
ORIGIN      
        1 acgctctttg gtgttttatg tccataaaat acgatgtcag aatttgaatt gttatcaaac
       61 ccaaggtaaa caacaaataa acaaagggta attaaaaaaa tgacaagttt gcctctaata
      121 atttgaagca tctgaactcc cttgctttgc ttttattgga ttatctgctt ttataccatc
      181 taacccgaag agttgcccaa tgttatattt ttcaatacat gcatcgctac atttggcaga
      241 aaaatcgcca caagcaatac cgacagtgtt atttttaaga tccatgattc tttctttagc
      301 tggctgacct tgtctatccc cagccgcctc atgattttta cctatcacgt cagctattgt
      361 tgatccaaat gtcttagtca ttcgacacat caagtaacaa tatctcattg catcggcggc
      421 cccattatga gctccgccaa caatagcatc catatcataa ccgaactggt aagcactgtt
      481 agtaatgctg tttgcaacaa tcaatccagg tacaccagat acggcataaa ttagtccacc
      541 tgatgtcata ttctcaatcg catctaaccc cattgggtct atcacttgta tgggattcaa
      601 cggatactga tacatattcc atccacctct caacccaatc gggtccgggg tgatataccg
      661 cccctgcaac ggatcgtagt accggtgacg gttatagtac agccccgact cctcatcatg
      721 ctgctggccc ggcagacggt acggctggtg caggtgatgc gggttctcct cattaagctg
      781 gttgccccat tcatcatatt ccgcgctcca cgccgtattg ccgtcttcgc tgataagcgc
      841 cagcggcagt ccccggtggt cacagtggta cagatgcagc gtgcgcgccg gcgtgtattc
      901 cggctccacc tgtttttcca gttgctccac cgtcagtccg cactgcgcaa gccacgcccg
      961 gctttcactg ctcacgcggt ctgcccggat ttcttcctcc agcctgtcca gcatcctcac
     1021 cagttctgcc gggaacacca caccgtgacc gtcctcactc ccttcctgct ggagtttctc
     1081 cgccaggctg cggcgctgcg ctttctcccg ctcgccgttc tccgtttcga tgcggatgag
     1141 cggtgtgaag ctccccggcc ggtataccgt ctggatacgt gtggtgccgg tctgtaccgt
     1201 cgtcagcctg tcgccgtccc acccgtacca ggtcacctcc ggtttacgcg acagcgacat
     1261 ccaccccgtc aggtcacgct cccgccgcca cacccgtttc cccgtccggc ggcccagcgg
     1321 gtcatagagg tagcggctct cgaccagcgg ctcgccgtat tgtatccgcg tgtagaacac
     1381 caggcggtgc tggttgtcgt aatggtagtg gtgggtccgc tcgtcgtgca tccggataac
     1441 cccttccgga atgcggtccg tcttctccgt cagcctgccg tattcatcgt agcggtagac
     1501 atagtgcgca tcttccgcga tgcggttatc cggccacgcc gtgagcgtgc tgtccggatg
     1561 cagttccggg tccggcagcc ggtttcctgc cgggtccgtt gcatacggga tatcgatatc
     1621 caggttcgct gccgtggtgt ggacgcccgt cagccttccc gtgtcgctgt aacggtactc
     1681 ccggctctcc tgcgggccgc tgatgcggat tagctgtccg ttgtcgttcc agtcgtagtc
     1741 acagtcaagc tgcgggaggt tcaggtgcct gctctgtagc tgccctgtga gggtgtacgc
     1801 cgtgacctgt tcgtaccccg ctgtactgcc cgccccgccg aagctgcggg ccgtctcacg
     1861 gtgc
//
LOCUS       DAZFAB010000265         1863 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZFAB010000265 DAZFAB010000000
VERSION     DAZFAB010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.268"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            4..654
                     /locus_tag="ACHJWA_004966"
     CDS             4..654
                     /locus_tag="ACHJWA_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFO4311375.1"
                     /translation="MPVILNFSNGSVLPENELEALRHIARSNQNDTITIGGRNMRLHY
                     IQFMDGFSVEPILGGLWDHLGAREAHHLADRLTRQLNGGNTFLQAYSLYLEQRQAAPL
                     VQESVIKTLLDRINSNAFPVSLQDFSCTEEHLNCPITLHIPETGVFVRNARNSEICAL
                     YDQEALTELILRNALHPLSRDPFAPEMIISKDKCHFNITKQCFYALPIYPLQQNSI"
     gene            733..1791
                     /gene="espW"
                     /locus_tag="ACHJWA_004967"
     CDS             733..1791
                     /gene="espW"
                     /locus_tag="ACHJWA_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspW"
                     /protein_id="HFO4311376.1"
                     /translation="MPKISSVVSSCYHLFSEHQQLSNETTMTNPVSRRIVHKEYGISL
                     KSVPVWLATAKTPLALLNGRHTRSHSFIIAGTPGMGSRSGAQYYAINSDDKRSRIDID
                     SLFLKKLNNVRNQNKFPIDVKETVIKLQGQKFTCIEDFYKRYNETRLKANTNIQQEQI
                     ADEVKSLTYLIPSEKKEMWIYKNNGKDNAKPNLGERDVRMFENISSDDTDKITGRKFS
                     ELGEYLYSGNVIKLSQLSIRYLPNISSISLIETKQSLLLHRLYSDEVLQRNGTLIPTP
                     LHEEKSIPADNIKTMLNNIPTYKMLPPFTETQGNCSSGAATFLRKSGAEEKDILACSP
                     RNYGLHHNIKTWDPLVRN"
BASE COUNT          684 a          353 c          327 g          499 t
ORIGIN      
        1 aatatgccag tcatattaaa tttttctaat ggaagtgtat tgccggaaaa tgagctggag
       61 gctttacgac atatagcaag aagcaaccag aatgacacta ttactatagg aggccgtaat
      121 atgaggcttc attatatcca gtttatggat ggttttagtg ttgagcctat tcttggtgga
      181 ctttgggacc atcttggggc aagagaggct catcatctcg cagaccgcct gacaagacaa
      241 cttaacggag gtaacacttt tctgcaggca tacagcttat atctggagca gaggcaagct
      301 gccccgcttg tgcaggagag cgtcataaaa acactactag atcgaataaa ttcgaatgca
      361 tttcccgtta gtttacaaga cttttcctgt actgaggaac atcttaattg tccgataacg
      421 ctacatattc ctgagacagg tgtttttgtc agaaatgctc gaaattcaga aatatgtgca
      481 ttatatgatc aggaagcatt gactgaactt atcctgcgta acgctctcca ccccctcagc
      541 cgtgacccct ttgctccaga aatgattata agcaaagaca agtgtcattt taatataaca
      601 aaacaatgtt tttacgcatt acccatatac ccacttcaac aaaacagtat ttaaaaataa
      661 aagcacaata taagaagcat attttcaata agttaatcac catataacaa acatatagcg
      721 aggagactat ttatgcccaa aatatcatca gttgtatcat catgttacca tctgttcagt
      781 gaacatcaac aactttcaaa tgaaacaaca atgacgaacc ccgtctccag aagaattgtt
      841 cataaagaat atggtatatc tttaaaatcc gttcctgtat ggttggctac agctaaaact
      901 ccccttgctc tactcaatgg cagacataca agaagtcact catttattat tgcagggacc
      961 ccaggaatgg gaagcaggag cggagcccaa tactatgcca taaacagtga tgataaacgc
     1021 tcccgaatag acattgactc tttattttta aaaaagttaa ataatgtgcg aaatcaaaat
     1081 aaatttccaa tcgatgtaaa agaaacggtt ataaaactac aagggcaaaa attcacatgc
     1141 attgaagatt tttataaaag gtataatgaa accaggttaa aggccaatac caatatccaa
     1201 caagaacaaa ttgcagatga agtaaaatca cttacatatt tgattccttc agaaaaaaaa
     1261 gagatgtgga tatataaaaa caatggaaaa gataatgcaa aaccaaactt aggagagcga
     1321 gacgtaagaa tgttcgaaaa tattagttct gatgatacag ataagataac aggaaggaaa
     1381 ttttcagagt taggtgagta tctttattca gggaatgtaa taaaactcag tcagttatca
     1441 attcgttact tacccaatat cagctcaatc tcattaatag agacaaaaca gagtttgttg
     1501 ctacatcgat tgtattcaga tgaagtactt cagagaaatg gaacgcttat cccgacacca
     1561 ctacatgaag aaaaatcaat tccagctgac aatataaaaa caatgctcaa caacataccg
     1621 acttacaaaa tgttaccgcc attcacagaa acacaaggta attgttcttc tggcgcagcc
     1681 acgtttttac gcaaatcagg cgccgaagaa aaagatattc ttgcatgtag cccccgaaat
     1741 tatgggctgc atcataacat aaaaacatgg gaccccttgg ttagaaatta aggatccaaa
     1801 tattatatat ttcatataaa gcaagaaaaa aatattgtct gaataattgt tcagacaata
     1861 tat
//
LOCUS       DAZFAB010000266         1863 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZFAB010000266 DAZFAB010000000
VERSION     DAZFAB010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.269"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..592)
                     /gene="nleH1-2"
                     /locus_tag="ACHJWA_004968"
     CDS             complement(<1..592)
                     /gene="nleH1-2"
                     /locus_tag="ACHJWA_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector kinase
                     NleH1-2"
                     /protein_id="HFO4311377.1"
                     /translation="MLSPSSINLGCSWNSLTRNLTSPDNRVLSSVRDAAVHSDSGTQV
                     TVGNRTYRVVVTDNKFCVTRESHSGCFTNLLHRLGWPKGEISRKIEAMLNTSPVSTTI
                     ERGSVHSNRPDLPPVDYAQPELPPADYTQSELPRVSNNKSPVPGNVIGKGGNAVVYED
                     MEDTTKVLKMFTISQSHEEVTSEVRCFNQYYGSGSAE"
     gene            complement(699..770)
                     /locus_tag="ACHJWA_004969"
                     /pseudo
     CDS             complement(699..770)
                     /locus_tag="ACHJWA_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
     gene            764..1758
                     /locus_tag="ACHJWA_004970"
                     /pseudo
     CDS             764..1758
                     /locus_tag="ACHJWA_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          567 a          444 c          394 g          458 t
ORIGIN      
        1 tctctgcact cccggaacca taatactgat tgaaacaacg aacttcgctt gtcacctctt
       61 catggctttg agatatagta aacatcttca acacttttgt tgtatcttcc atatcttcat
      121 acacgacagc attaccacct ttaccaataa cattacctgg cacgggtgat ttattgttgc
      181 taaccctcgg caactctgat tgagtataat ccgctggagg taactccggc tgcgcataat
      241 ccactggagg taaatcaggt ctgttcgaat gaacagagcc tctttctata gtcgtgctca
      301 ctggcgatgt attcagcata gcctcaattt ttctgctaat ctctccctta ggccatccca
      361 acctgtgcaa cagattagta aaacaaccac tatgactttc tcttgtaacg caaaacttat
      421 tatcagtgac cacaacacga tatgttctgt tgccaaccgt tacttgcgtc ccgctatcag
      481 agtgaacagc agcatccctt acagaggata aaacacgatt atcaggcgaa gtcaggtttc
      541 tggttaaaga attccatgaa catcccaaat ttatagaaga gggcgataac atacatttca
      601 accttcaaaa taaacctatc taattattcc taacagacat cccccataca tgacaacaaa
      661 aaccggagcc ggactccggt ttttgtgaag ctatcgggtt acttcatttc gccaatattt
      721 tcccacttcc cgtcagcacg caggatttgc agcggtctta ctacacactg acccgaggac
      781 gaaaaaagag ttcgggctgg gaagagacag aagagtagca atttcagaag cattcacacc
      841 aacattgagc ttttcagcga aagtgggcac gaatcattgc tggacagggt taaaggcact
      901 gactcaatca ctcttcatac gtggcttgat cgatatgaaa caatcctcag ctagaggggg
      961 agcaagccga aaacattact cgactacgcc agcaaaatca gggcaattcg aagaaaattg
     1021 ccggacaaac cgctcactga catatcaacg aaagaggtgg cagcaatgct aaacacctac
     1081 gtcgcagaag gtaaagcggt ttccgcaaga gtaatcaggt caacccttgt tgacgttttt
     1141 cgaggggcaa tagccgaggg gcatgtggca acgaatccag taacaacaac ccgtgcagca
     1201 aagtcagaag taaggcgctc aaggctgaca gctaatgagt atgtcgctat ttaccatgct
     1261 gctgagcacc tccccatctg gctgaggctg tcaatggatt tagctgtcgt tacagggcag
     1321 agagtgggcg atttgtgcag aatgaagtgg tcagacataa acgatggtca tcttcacatt
     1381 ggacagagta aaacaggagc caaaattgcc attccgctgg ctctaaccat tgacgcactc
     1441 gacatctcac tggttgatac actacagaaa tgcagggagg ccagcagcag tgaaacaata
     1501 atcgcatcaa cctatcacga accactttct ccagccacag tatcacggta tttaacaaag
     1561 gcgcgaaatg catccgggat ctcgtttgat ggagacccac cgacatttca tgaactacgt
     1621 agtctgtccg cgaggctata tcggaaccag attggctaca agtttgcaca acgtcttctt
     1681 ggacataaat ctgattcaat ggcggcgcat tatagggaca gtcgcgggcg ggagtgggac
     1741 aaaattgaaa tcggataatg attttatttt gaccaataat gacttaccag atttaacaac
     1801 ttgatattta ataagatttt tgaacgaaca acttccatgt cagaaggagg ataaaatcac
     1861 aaa
//
LOCUS       DAZFAB010000267         1839 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZFAB010000267 DAZFAB010000000
VERSION     DAZFAB010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.270"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            176..>1839
                     /gene="tir"
                     /locus_tag="ACHJWA_004971"
     CDS             176..>1839
                     /gene="tir"
                     /locus_tag="ACHJWA_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocated
                     intimin receptor Tir"
                     /protein_id="HFO4311378.1"
                     /translation="MPIGNLGHNPNVNNSIPPAPPLPSQTDGAGGRGQLINSTGPLGS
                     RALFTPVRNSMADSGDNRASDVPGLPVNPMRLAASEITLNDGFEVLHDHGPLDTLNRQ
                     IGSSVFRVETQEDGKHIAVGQRNGVETSVVLSDQEYARLQSIDPEGKDKFVFTGGRGG
                     AGHAMVTVASDITEARQRILELLEPKGTGESKGAGESKGVGELRESNSGAENTTETQT
                     STSTSSLRSDPKLWLALGTVATGLIGLAATGIVQALALTPEPDSPTTTDPDAAASATE
                     TATRDQLTKEAFQNPDNQKVNIDELGNAIPSGVLKDDVVANIEEQAKAAGEEAKQQAI
                     ENNAQAQKKYDEQQAKRQEELKVSSGAGYGLSGALILGGGIGVAVTAALHRKNQPVEQ
                     TTTTTTTTTTTSARTVENKPANNTPAQGNVDTPGSEDTMESRRSSMASTSSTFFDTSS
                     IGTVQNPYADVKTSLHDSQVPTSNSNTSVQNMGNTDSVVYSTIQHPPRDTTDNGARLL
                     GNPSAGIQSTYARLALSGGLRHDMGGLTGGSNSAVNTSNNPPAPGS"
BASE COUNT          515 a          394 c          475 g          455 t
ORIGIN      
        1 tacccgatgt gtgtttttag cacattattt tcttgcatca aaaatcagat tgtgatttat
       61 ttggtcgatg tcatgttcag ccgtttatcg actacgtgct gtataccata cattattatg
      121 taatagcaaa ataaaggatt gttatctatt tacataaaat aaaaaggaga tatttatgcc
      181 tattggtaat cttggtcata atcccaatgt gaataattca attcctcctg cacctccatt
      241 accttcacaa accgacggtg caggggggcg tggtcagctc attaactcta cggggccgtt
      301 gggatctcgt gcgctattta cgcctgtaag gaattctatg gctgattctg gcgacaatcg
      361 tgccagtgat gttcctggac ttcctgtaaa tccgatgcgc ctggcggcgt ctgagataac
      421 actgaatgat ggatttgaag ttcttcatga tcatggtccg ctcgatactc ttaacaggca
      481 gattggctct tcggtatttc gagttgaaac tcaggaagat ggtaaacata ttgctgtcgg
      541 tcagaggaat ggtgttgaga cctctgttgt tttaagtgat caagagtacg ctcgcttgca
      601 gtccattgat cctgaaggta aagacaaatt tgtatttact ggaggccgtg gtggtgctgg
      661 gcatgctatg gtcaccgttg cttcagatat cacggaagcc cgccaaagga tactggagct
      721 gttagagccc aaagggaccg gggagtccaa aggtgctggg gagtcaaaag gcgttgggga
      781 gttgagggag tcaaatagcg gtgcggaaaa caccacagaa actcagacct caacctcaac
      841 ttccagcctt cgttcagatc ctaaactttg gttggcgttg gggactgttg ctacaggtct
      901 gatagggttg gcggcgacgg gtattgtaca ggcgcttgca ttgacgccgg agccggatag
      961 cccaaccacg accgaccctg atgcagctgc aagtgcaact gaaactgcga caagagatca
     1021 gttaacgaaa gaagcgttcc agaacccaga taatcaaaaa gttaatatcg atgagctcgg
     1081 aaatgcgatt ccgtcagggg tattgaaaga tgatgttgtt gcgaatatag aagagcaggc
     1141 taaagcagca ggcgaagagg ccaaacagca agccattgaa aataatgctc aggcgcaaaa
     1201 aaaatatgat gaacaacaag ctaaacgcca ggaggagctg aaagtttcat cgggggctgg
     1261 ctacggtctt agtggcgcat tgattcttgg tgggggaatt ggtgttgccg tcaccgctgc
     1321 gcttcatcga aaaaatcagc cggtagaaca aacaacaaca acaactacta caactacaac
     1381 tacaagcgca cgtacggtag agaataagcc tgcaaataat acacctgcac agggcaatgt
     1441 agatacccct gggtcagaag ataccatgga gagcagacgt agctcgatgg ctagcacctc
     1501 gtcgactttc tttgacactt ccagcatagg gaccgtgcag aatccgtatg ctgatgttaa
     1561 aacatcgctg catgattcgc aggtgccgac ttctaattct aatacgtctg ttcagaatat
     1621 ggggaataca gattctgttg tatatagcac cattcaacat cctccccggg atactactga
     1681 taacggcgca cggttattag gaaatccaag tgcggggatt caaagcactt atgcgcgtct
     1741 ggcgctaagt ggtggattac gccatgacat gggaggatta acggggggga gtaatagcgc
     1801 tgtgaatact tcgaataacc caccagcgcc gggatccca
//
LOCUS       DAZFAB010000268         1784 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZFAB010000268 DAZFAB010000000
VERSION     DAZFAB010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.271"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..1632
                     /locus_tag="ACHJWA_004972"
     rRNA            <1..1632
                     /locus_tag="ACHJWA_004972"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          452 a          402 c          557 g          373 t
ORIGIN      
        1 taaagcgggt gaaaagcccg ctcgccggaa gaccaagggt tcctgtccaa cgttaatcgg
       61 ggcagggtga gtcgacccct aaggcgaggc cgaaaggcgt agtcgatggg aaacaggtta
      121 atattcctgt acttggtgtt actgcgaagg ggggacggag aaggctatgt tggccgggcg
      181 acggttgtcc cggtttaagc gtgtaggctg gttttccagg caaatccgga aaatcaaggc
      241 tgaggcgtga tgacgaggca ctacggtgct gaagcaacaa atgccctgct tccaggaaaa
      301 gcctctaagc atcaggtaac atcaaatcgt accccaaacc gacacaggtg gtcaggtaga
      361 gaataccaag gcgcttgaga gaactcgggt gaaggaacta ggcaaaatgg tgccgtaact
      421 tcgggagaag gcacgctgat atgtaggtga agtccctcgc ggatggagct gaaatcagtc
      481 gaagatacca gctggctgca actgtttatt aaaaacacag cactgtgcaa acacgaaagt
      541 ggacgtatac ggtgtgacgc ctgcccggtg ccggaaggtt aattgatggg gtcagcgcaa
      601 gcgaagctct tgatcgaagc cccggtaaac ggcggccgta actataacgg tcctaaggta
      661 gcgaaattcc ttgtcgggta agttccgacc tgcacgaatg gcgtaatgat ggccaggctg
      721 tctccacccg agactcagtg aaattgaact cgctgtgaag atgcagtgta cccgcggcaa
      781 gacggaaaga ccccgtgaac ctttactata gcttgacact gaacattgag ccttgatgtg
      841 taggataggt gggaggcttt gaagtgtgga cgccagtctg catggagccg accttgaaat
      901 accacccttt aatgtttgat gttctaacgt ggacccgtga tccgggttgc ggacagtgtc
      961 tggtgggtag tttgactggg gcggtctcct cctaaagagt aacggaggag cacgaaggtt
     1021 ggctaatcct ggtcggacat caggaggtta gtgcaatggc ataagccagc ttgactgcga
     1081 gcgtgacggc gcgagcaggt gcgaaagcag gtcatagtga tccggtggtt ctgaatggaa
     1141 gggccatcgc tcaacggata aaaggtactc cggggataac aggctgatac cgcccaagag
     1201 ttcatatcga cggcggtgtt tggcacctcg atgtcggctc atcacatcct ggggctgaag
     1261 taggtcccaa gggtatggct gttcgccatt taaagtggta cgcgagctgg gtttagaacg
     1321 tcgtgagaca gttcggtccc tatctgccgt gggcgctgga gaactgaggg gggctgctcc
     1381 tagtacgaga ggaccggagt ggacgcatca ctggtgttcg ggttgtcatg ccaatggcac
     1441 tgcccggtag ctaaatgcgg aagagataag tgctgaaagc atctaagcac gaaacttgcc
     1501 ccgagatgag ttctccctga ccctttaagg gtcctgaagg aacgttgaag acgacgacgt
     1561 tgataggccg ggtgtgtaag cgcagcgatg cgttgagcta accggtacta atgaaccgtg
     1621 aggcttaacc ttacaacgcc gaaggtgttt tggcggatga gagaagattt tcggcctgat
     1681 acagattaaa tcagaacgca gaagcggtct gataaaacag aatttgcctg gcggccttag
     1741 cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg aaac
//
LOCUS       DAZFAB010000269         1774 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZFAB010000269 DAZFAB010000000
VERSION     DAZFAB010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.272"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(33..482)
                     /gene="sctT"
                     /locus_tag="ACHJWA_004973"
                     /pseudo
     CDS             complement(33..482)
                     /gene="sctT"
                     /locus_tag="ACHJWA_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503299.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
     gene            complement(484..744)
                     /locus_tag="ACHJWA_004974"
     CDS             complement(484..744)
                     /locus_tag="ACHJWA_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO4311379.1"
                     /translation="MDDIVFAGNRALYLILVMSAGPIAVATFVGLLVGLFQTVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            complement(755..1420)
                     /locus_tag="ACHJWA_004975"
     CDS             complement(755..1420)
                     /locus_tag="ACHJWA_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO4311380.1"
                     /translation="MSNSISLIAILSLFTLLPFIIASGTCFIKFSIVFVIVRNALGLQ
                     QVPSNMTLNGVALLLSMFVMMPVGKEIYYNSQNENLSFNNVASVVNFVETGMSGYKSY
                     LIKYSEPELVSFFEKIQKVNSSEDNEEIIDDDNISIFSLLPAYALSEIKSAFIIGFYI
                     YLPFVVVDLVISSVLLTLGMMMMSPVTISTPIKLILFVAMDGWTMLSKGLILQYFDLS
                     INP"
     gene            complement(1410..>1774)
                     /gene="sctQ"
                     /locus_tag="ACHJWA_004976"
     CDS             complement(1410..>1774)
                     /gene="sctQ"
                     /locus_tag="ACHJWA_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system cytoplasmic ring
                     protein SctQ"
                     /protein_id="HFO4311381.1"
                     /translation="IYPEELKMADHFQYEEDFETDDFDIKKSESEIYDENDEQMINSF
                     EELPVKIEFVLGKKIMNLYEIDELCAKRIISLLPESEKNIEIRVNGALTGYGELVEVD
                     DKLGVEIHSWLSGHNNVK"
BASE COUNT          606 a          387 c          260 g          521 t
ORIGIN      
        1 cttcctgaag gccataaatt ataactttgc taaagagttt ccagaataaa gttcaatcca
       61 ccgttagtaa gataaaccgc tgcagagaaa aggttaaata atcttgctaa ttccgaggta
      121 tcgacacctg tagccggatc caaagtacta cttaacgttg caccgcgttg gttatcgata
      181 aagctaccta tagcaaggaa aatccaaaat ggagatgcta ataagcaagc aatgaacagc
      241 cctatgataa tttctcttgc agctaacaaa gcgatatcga tttcatttaa ggtcgataat
      301 tcatagtggt aatgctgaac taatgcacat gataccacta ttattattgg atgacggact
      361 actgttggta tattcccact ggcgaaaaat ggtaacaaat agaaagttgg tgccaatcgg
      421 caaaaaccga gcgctgtagc agccagtaga ctatgcaact gatagagtat cacctccccc
      481 atgctatcct ctcgcaaatg ctaaattcag catttcaata ccaaaagaat ataatttttc
      541 accataccat ccgctcatta aaaaaaagca aatacttacg cacaataatt tcacgccaaa
      601 tggcaacgtc tgttcttgca actgcgttac cgtttgaaaa agccctacca ataaaccaac
      661 aaatgtcgct actgcaatag gtcctgcaga cataactaaa atcagataca gcgccctatt
      721 ccctgcaaaa acaatgtcat ccatattttt ttcctcaggg attaatggat aggtcaaaat
      781 attgtaatat gagtcctttt gataacattg tccaaccgtc catcgcgaca aacagaatta
      841 attttatcgg tgtagatatg gttaccggac tcatcatcat catacctaac gttaacagaa
      901 cgctggatat aaccagatcc actacaacaa agggtaaata gatataaaaa cctataataa
      961 atgcactttt tatctcactt aacgcatatg ccgggagtaa cgagaaaatt gaaatattat
     1021 catcatcgat tatctcttca ttgtcttcag aactattcac cttctgaatc ttttcaaaaa
     1081 agctaaccaa ctccggttca gaatatttta tcaaatatga tttataacca ctcatgcctg
     1141 tctcaacaaa attaacaacc gacgctacat tattgaatga aagattctca ttctgactgt
     1201 tataatatat ttccttccca accggcatca ttacaaacat tgacaatagc aaagcaaccc
     1261 cattaagtgt catatttgat ggcacttgct gaagtcccaa tgcgtttcgg acaataacaa
     1321 aaacaatcga gaatttaata aaacatgtcc cggatgctat tataaacggc aatagtgtaa
     1381 ataatgatag tatcgctatc agcgaaatgc tatttgacat tattatgccc tgataaccaa
     1441 gaatgaatct ctacgcccaa tttatcatcc acttcaacaa gttcgccata gccagttagc
     1501 gcgccattta cacgtatctc tatatttttc tcagattcag gaagcagaga tattattctt
     1561 tttgcacaga gctcgtctat ttcatataga ttcattattt tcttaccaag aacaaactca
     1621 atttttacag gcaactcttc aaaactatta atcatctgtt cgtcattttc atcataaatc
     1681 tcactctcgc tttttttgat atcaaagtca tctgtttcaa aatcttcctc gtactgaaaa
     1741 tgatcagcca tttttaactc ctctggataa atta
//
LOCUS       DAZFAB010000270         1709 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZFAB010000270 DAZFAB010000000
VERSION     DAZFAB010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.273"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..206)
                     /locus_tag="ACHJWA_004977"
     CDS             complement(<1..206)
                     /locus_tag="ACHJWA_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO4311382.1"
                     /translation="MKIKHEHIRMAMNAWARPDGEKVPAAGITQAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWIEKDTPDA"
     gene            complement(190..432)
                     /locus_tag="ACHJWA_004978"
     CDS             complement(190..432)
                     /locus_tag="ACHJWA_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO4311383.1"
                     /translation="MKKSEVLGYFGGVVKTAAALGTSKTTVSMWGEDVPWKWALLIQA
                     VTAGALKYELHIPTVVIPDSDHNPPSNQGGIHENQA"
     gene            824..1162
                     /locus_tag="ACHJWA_004979"
     CDS             824..1162
                     /locus_tag="ACHJWA_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFO4311384.1"
                     /translation="MKNSSITSCVQLVGEIPANTFAVVLESDSMSTSGGGVSIPNGST
                     VFVDPDRIVQPGNIVLALPKGTTTPVIRKLEIEGPDILLVPTNPRYPSIMLDDLSCIL
                     GVCFKIQQDI"
     gene            1455..1607
                     /locus_tag="ACHJWA_004980"
     CDS             1455..1607
                     /locus_tag="ACHJWA_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO4311385.1"
                     /translation="MDTLNLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LARNTD"
     gene            1619..>1709
                     /locus_tag="ACHJWA_004981"
     CDS             1619..>1709
                     /locus_tag="ACHJWA_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311386.1"
                     /translation="MEFKDLPPSIQEIAAHTLRHRLNELELESV"
BASE COUNT          485 a          401 c          375 g          448 t
ORIGIN      
        1 gcatcagggg tgtctttctc tatccagcgg aaaattttct gggtattgcg agccagggct
       61 tccggatggc tgtcgtcgta cagttcagga aacgtcatac ccaactcaaa ataagcctgg
      121 gttattccag ctgccggaac tttttcgcca tcaggacgcg cccaggcatt catcgccatg
      181 cggatgtgtt catgcttgat tttcatgaat ccccccttgg ttagaaggcg gattatgatc
      241 agaatcggga atgacaaccg tcggtatgtg taactcatat ttgagcgccc cggcagtgac
      301 tgcctgaatt agcaacgccc atttccacgg aacgtcttcc ccccacatgc tgactgtggt
      361 ttttgacgtt cctagagcgg cggctgtttt aacaactccg ccaaaatagc ctaatacttc
      421 tgattttttc atgagtcgct ccataaaact gaacgccaaa agtttaataa tcaaaaccaa
      481 agaaagtcaa gaaacaaaac catctgtgtt ttaaaatcaa aacatgaaca agcaaacaat
      541 atctgaacgc ataacccaac gtatgcatgc gctaaacttg aaaggcaaag accttgtcaa
      601 tgccactggc gcatcaaaag gctccgtaag tcaatggatg aacggtggag gagcgccgtc
      661 ctcgcgttac ataagttcac tggcaaagat attgaaagta aacgaaaatt ggcttcttaa
      721 tggaggagag ttaaatacag gtgattcgct tgatctatct ttaccgccga taaaaacggt
      781 tccgctacta tcacttcagc aggcagcaag ctggagtgat tatatgaaaa attcctcaat
      841 aacctcttgt gtgcagcttg tcggagaaat cccggccaat acctttgctg ttgttctaga
      901 gagtgacagt atgtcgacat ctggtggtgg agtttccatc ccaaatggtt caacagtttt
      961 tgttgatccc gatcgaatcg tacaaccagg aaatattgtc cttgccttac ccaaagggac
     1021 cacaacgcct gtcattcgca aactggagat agaagggccg gatattcttt tagtccccac
     1081 gaatcctcgc tacccttcaa ttatgctgga tgatctatct tgcatattgg gagtatgctt
     1141 taaaattcaa caagatattt aaccaacctc atctatttga ttaactgtat gccatcgtag
     1201 tgatggcgta acagctgcct gcttaaaatg ttttgataaa aaaacattga ccttaaatgt
     1261 tcgtttttct aaacttcatt cattccctca ccccgcccca cagaatgcag ggcaatactt
     1321 cgagttacca ggcagtggtc aggggttaag tagccagccc gaggcgtaag aacatgacgg
     1381 cagggttcaa ctttaataac tatgcagcag gtttttgttc cgctaccccg gcgttaaggg
     1441 gaaatgaggt caacatggat acgctcaatc ttggcaacaa cgaatctctg gtatgtggcg
     1501 tgttccccaa ccaggacggt acgttcaccg cgatgacgta taccaaaagc aaaacgttta
     1561 aaactgaagc tggcgcgcgt cgctggttag ccagaaacac tgactgatga ggttgacgat
     1621 ggaatttaaa gatttacctc cttcaatcca ggagattgca gcacacacac ttcgtcatcg
     1681 tctgaacgaa cttgaattgg aatcggtaa
//
LOCUS       DAZFAB010000271         1708 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZFAB010000271 DAZFAB010000000
VERSION     DAZFAB010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.274"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(237..743)
                     /gene="espH"
                     /locus_tag="ACHJWA_004982"
     CDS             complement(237..743)
                     /gene="espH"
                     /locus_tag="ACHJWA_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspH"
                     /protein_id="HFO4311387.1"
                     /translation="MSLSGAVFKTFLTSEHASWNRFNRRLHIPNEDIVDEIQLKARMQ
                     QRHHRVYPEIGDSTIVSFRGKDYAVHFIKDGPKDDYVYKVQRITPENGCFSTLFSVFS
                     GGVTKALERKLNERHITPLSSTWFPRTPLEGILAERGLSSLLRRVQSTERLDNRAIAT
                     RASSYSVL"
     gene            complement(774..1664)
                     /gene="escQ"
                     /locus_tag="ACHJWA_004983"
     CDS             complement(774..1664)
                     /gene="escQ"
                     /locus_tag="ACHJWA_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ring protein EscQ"
                     /protein_id="HFO4311388.1"
                     /translation="MKINDFYLPLLPVIGTGRLYITSKGHACHAYFREVSGHGIRFTL
                     TYSGYEGRFWISEEQFIQWCQELFPYSESRLIPEDTIKLMILWVMQTALPEGDVSVDD
                     VQFTMLNKDIYPVIENNNGENRLNVIILETTVQSLQYLINDNWQLVPHSNALFFDGYI
                     VPGWTDYPVTELRVGDSLRLYHVDDSQERRCWIVINTPLATVNLSDNNLSVTDVLAAD
                     LLSALSNETVMNRIYCAIGTVHIDIHMLRNVKKDDIINSDGYHLFGGCQLIRNNTTIA
                     YGSIVKINEDFYFTVSIVCD"
     gene            complement(1654..1701)
                     /locus_tag="ACHJWA_004984"
     CDS             complement(1654..1701)
                     /locus_tag="ACHJWA_004984"
                     /inference="COORDINATES: protein motif:HMM:NF018255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1106 domain-containing protein"
                     /protein_id="HFO4311389.1"
                     /translation="MFPDEAIEFTIEYEN"
BASE COUNT          531 a          368 c          288 g          521 t
ORIGIN      
        1 agatccttgt cttccagacc tacagatttt ttaccttgat aatttctgcg ctggatggga
       61 tcatccattc cgtttaacat actcttcatc acagggacct ctgatttctg gccgctaacg
      121 tctctgaaga ttcagcctgt attatctaaa agcaatgtaa tatcagttaa aaagcggaaa
      181 aactccagaa ttataactat tctggagtta agtcaggaag aaaactcaaa tgctaatcat
      241 aatacgctat aagaggaagc tcttgttgct atagccctat tatcaagacg ttcggtagac
      301 tgtacacgac gtaacaaaga agataatcct ctttcagcta agatcccctc caggggagta
      361 cgaggaaacc aggttgaaga aagtggagtt atatgtctct catttaattt tctttccaaa
      421 gccttagtca caccgccaga aaacacgcta aatagcgttg agaaacatcc attttccggt
      481 gttattcttt gtactttgta cacataatca tcttttgggc catctttaat aaagtgaaca
      541 gcatagtctt tgcctctaaa agagacaatg gtggaatccc ctatttctgg atatacccga
      601 tgatggcgtt gttgcattcg ggctttaagc tgaatttcat ctacgatatc ttcattgggt
      661 atatgaagcc ttctgttgaa cctgttccat gacgcatgtt cacttgtcag aaatgtcttg
      721 aataccgctc ctgataacga catgatacat ctccctatat aacgtattcc tgattaatca
      781 catactatgc taacagtaaa ataaaaatct tcgttgattt ttacaatact cccgtatgct
      841 atcgtcgtat tattgcgtat taactgacaa ccaccaaaaa gatggtatcc atctgaatta
      901 ataatgtcat cttttttaac attacgcaac atgtgtatat caatatggac tgtaccgatc
      961 gcacaataaa tgcgattcat gacagtctca ttgctaagag cagataacag atccgcagcc
     1021 agaacatccg tgacagataa attattatca ctcagattta ctgttgctaa aggggtatta
     1081 ataacaatcc agcaccttct ctcttgacta tcatcaacat gatagagtct cagactatca
     1141 ccaactctca actctgtaac tggataatca gtccacccag gtacgatata gccatcaaaa
     1201 aaaagagcat tagaatgtgg aactaattgc caattatcat taatcagata ttgtaaactt
     1261 tgtaccgttg tttcgagtat aattacatta agtctgtttt ctccattatt attttctatt
     1321 accggataaa tatctttatt tagcatcgta aattgaacat catcaactga cacatcacct
     1381 tccggtaagg cagtctgcat tacccataag atcatgagtt ttatcgtatc ttccggtatc
     1441 agccggcttt cgctatacgg gaataactct tggcaccact gaatgaactg ttcttctgat
     1501 atccagaaac gcccctcata gcccgagtat gtcaacgtga aacgaatacc atgaccgctg
     1561 acctcacgaa aatatgcatg gcaagcatga cctttggacg taatatagag gcgaccggtt
     1621 ccgataactg gcaacaacgg caaataaaaa tcgttaattt tcatattcaa ttgtgaactc
     1681 aatggcttca tcaggaaaga tttctttt
//
LOCUS       DAZFAB010000272         1708 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZFAB010000272 DAZFAB010000000
VERSION     DAZFAB010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.275"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            77..1177
                     /gene="ompC"
                     /locus_tag="ACHJWA_004985"
     CDS             77..1177
                     /gene="ompC"
                     /locus_tag="ACHJWA_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO4311390.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRDNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGVATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGIDLGRYGDQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            1340..>1708
                     /locus_tag="ACHJWA_004986"
     CDS             1340..>1708
                     /locus_tag="ACHJWA_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFO4311391.1"
                     /translation="MIMTNINTACVKNNASYQFNNALPNKETISSNFCERLEQWGNKS
                     LNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDFT
                     DNASLVNINLSFNKINTITFP"
BASE COUNT          511 a          347 c          350 g          500 t
ORIGIN      
        1 tatgtcattt ataaatggcg ccgtttaaaa ttcgtttaaa aaatacgttt aacgataatt
       61 aaaaggattc tgttaaatga aattaaaaat agttgcggtg gttgtaactg gtttgttagc
      121 tgcgaacgta gcacacgctg ccgaagtcta taacaaggat ggtaataaac tcgaccttta
      181 tggcaaggtt accgctctac gttattttac tgatgataag cgtgacgatg gtgataaaac
      241 ttatgcccgt ctcggcttta aaggagaaac gcaaatcaat gatcaaatga ttggttttgg
      301 tcactgggaa tatgatttta aaggctataa cgatgaagcc aacggctcgc gcgacaacaa
      361 gacccgtctg gcctatgctg gtttaaaaat tagtgaattt ggctctctgg actatggccg
      421 taactacggt gtcggctatg acattggttc atggactgat atgttgccag aatttggtgg
      481 cgatacctgg agtcagaaag atgtcttcat gacataccgt accaccggtg tagcaaccta
      541 tcgcaactac gatttctttg gcttaattga agggctgaac tttgccgcgc aatatcaagg
      601 caaaaatgaa cgtactgaca acagtcatct ttatggtgct gactacacgc gtgccaacgg
      661 tgacggtttc ggtatctcct caacttatgt ttatgatggc tttggtatcg gagcggtgta
      721 taccaaatcc gatcggacaa atgcgcagga aagagccgct gctaatcctc tcaatgcctc
      781 cggtaagaat gcagaactgt gggctacagg tataaaatat gatgccaaca acatctactt
      841 tgcagctaat tacgctgaaa cattaaacat gaccacctat ggcgatggtt atatttctaa
      901 caaagcacaa agttttgaag tggtggcgca atatcaattc gacttcggct tgcgcccctc
      961 actcgcttac ctgaaatcga aaggcataga tctgggccgc tacggcgatc aggacatgat
     1021 tgagtatatc gacgttggtg cgacgtattt cttcaacaaa aatatgtcga cctatgttga
     1081 ttataaaatc aacctgattg atgaaagcga ctttacccgt gccgtagata ttcgcaccga
     1141 taacatcgtc gctacgggca ttacctatca gttctaacat cctctcctga ccagcccttc
     1201 tctgggctgg tttttgattt aagtgctgct gtatgactcc ttaagtaaaa atgagcaatt
     1261 tttgatattt ctggtcattc acctcaatta atattattcc atacccccgg ggacatctta
     1321 ctataatatt tattgatgag tgattatgac caatattaat acagcttgtg taaaaaataa
     1381 tgccagttat caatttaata acgcattacc taacaaagaa accatctcca gcaatttttg
     1441 tgaacgacta gaacaatggg gtaataagtc gcttaataat ggtgaagaaa gagcaattgc
     1501 cgtggagcga attaaagaag cttacaattc gaacatggca tctcttgatt tgtcatatct
     1561 tgatttgagt gagttaccgc ctatcccctc gacagttaat acgttaaatc tggaaaacaa
     1621 ttgtctaact tgtcttgact ttactgacaa tgccagtctc gttaatatca acctcagctt
     1681 taataaaatt aacacgataa cctttcca
//
LOCUS       DAZFAB010000273         1677 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZFAB010000273 DAZFAB010000000
VERSION     DAZFAB010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.276"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            228..>1677
                     /locus_tag="ACHJWA_004987"
     CDS             228..>1677
                     /locus_tag="ACHJWA_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HFO4311392.1"
                     /translation="MVDNVTVSRVCIQSPSFVPDLDGEKNKSQLFVDDIVAYLKSPSV
                     YSLEKEGPLNHFVNHCSEVELGFYSDGAYSILVSRSKQQPEGMILTVSDADAINIVHI
                     SVSPVLIKFLDDIFTCLHTYPDDESFTKEQIKANSKYDIVDYNCLLHFTGKPKSLIEC
                     RHFALQYCIDSMNEHTGKVPLKAYYSSPEDIQKHIPFELEQQFNNLQKNPPPGTCVVA
                     SDKFGEALSVFFHRMEKEKLTHMTAIVQSQTHAMAVRLRIKKTPAGETEYVVSFYDPN
                     ATNTAVRYKANNCDSFGSLQSFINIQQAKQKWVITDICSECVGITPYLPREQAHLLSG
                     IENELQPPLSPPALFLLMRMGIYKNIVLFFDKLKNSQEMTASKALDILAAKSPEGIYG
                     LCVLLYHNTIDKFNDYITNLKELTRKYNFSQEDLETLLLAKDNLGVSWIPRALKNNQN
                     KIVKAWLLAIDDFEKEFGVNKNEILLRIGKEID"
BASE COUNT          574 a          275 c          314 g          514 t
ORIGIN      
        1 ttataattat tgttgaagat gatgatattg attataggga taccattaca aggagctgtt
       61 cccctcgttg acgtcgcggt ttacccacct cattaccagg ttgatgacct gatggggaaa
      121 tgacgtaaaa agacaaataa ctctataaat gagcgaaatt tgatagttga tttttaaata
      181 gtaaccttat tattactaca actttcatat ggatgagggg aaagaaaatg gttgataacg
      241 taactgttag cagagtatgt atacaatctc cttctttcgt gcctgatttg gatggagaaa
      301 aaaataaatc tcaattattc gttgacgata tagttgcgta tcttaaaagt ccttcagttt
      361 attcacttga aaaagagggg ccgttaaatc attttgttaa tcattgttct gaagttgagt
      421 taggtttcta tagcgatggt gcatattcaa ttcttgtctc cagatctaag caacaacctg
      481 aaggtatgat tttaaccgta agcgatgcag acgcaatcaa tatagtacat atttccgtat
      541 ctccagtgct tataaaattc ctggatgata tttttacttg ccttcatacg taccctgatg
      601 atgagagttt tacaaaagag cagataaaag ctaatagcaa atatgatatt gtagattata
      661 attgcctgtt gcattttact ggaaaaccaa aaagtttaat agaatgtaga cattttgctc
      721 tgcaatactg tatagattca atgaatgagc atacagggaa agttccatta aaggcttact
      781 attcatctcc ggaagatata caaaaacata ttcctttcga gcttgagcag caatttaaca
      841 atctacaaaa aaatccacca cccggtacat gcgtcgttgc cagtgataag tttggggagg
      901 cattatctgt cttttttcac agaatggaaa aagagaagtt aacgcatatg acggcaatcg
      961 ttcaatctca aacacacgct atggccgtcc gcttgaggat caaaaaaacg cctgctggtg
     1021 aaacagaata tgttgtatcc ttttatgatc ctaatgcaac caatactgca gtacgctata
     1081 aagcaaacaa ctgtgattct tttgggtcat tgcaatcgtt tataaatatt caacaggcaa
     1141 aacaaaaatg ggtaataaca gatatttgct ccgagtgtgt aggaataacc ccttatctcc
     1201 ctcgggaaca agcccattta ttaagtggta ttgaaaatga gttgcaacct ccattatcac
     1261 caccagcatt atttctatta atgagaatgg ggatatataa aaacattgtt ctttttttcg
     1321 ataaattaaa aaactctcaa gaaatgacag catcaaaggc tcttgatatt cttgctgcga
     1381 aatcacctga aggaatatat gggttatgtg tattattgta tcacaatact attgataagt
     1441 ttaatgatta cataacaaat ttaaaagagt tgaccagaaa atataatttt agccaagagg
     1501 acctggaaac tctactcctt gcgaaagata atctcggagt gagctggatt cccagggctt
     1561 tgaaaaataa tcaaaataaa attgtcaaag catggttgtt ggcgatagat gactttgaga
     1621 aagaatttgg ggtaaataaa aatgaaatac ttcttcgtat aggaaaggaa atagact
//
LOCUS       DAZFAB010000274         1670 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZFAB010000274 DAZFAB010000000
VERSION     DAZFAB010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.277"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            297..458
                     /locus_tag="ACHJWA_004988"
                     /pseudo
     CDS             297..458
                     /locus_tag="ACHJWA_004988"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            442..507
                     /locus_tag="ACHJWA_004989"
     CDS             442..507
                     /locus_tag="ACHJWA_004989"
                     /inference="COORDINATES: protein motif:HMM:NF033646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAST1 family heat-stable enterotoxin"
                     /protein_id="HFO4311393.1"
                     /translation="MWCATACASCHGRNTKPSLAT"
     gene            623..880
                     /locus_tag="ACHJWA_004990"
     CDS             623..880
                     /locus_tag="ACHJWA_004990"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO4311394.1"
                     /translation="MATFFAYPADIRKVIYTTNAIESLNSVIRHAIRKRKVFPTDESV
                     KKVVWLAIQAASQKWTMPLRDWRMAMSRFIIEFGDRLDGHF"
     gene            complement(927..1496)
                     /gene="nleF"
                     /locus_tag="ACHJWA_004991"
     CDS             complement(927..1496)
                     /gene="nleF"
                     /locus_tag="ACHJWA_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector caspase inhibitor NleF"
                     /protein_id="HFO4311395.1"
                     /translation="MLPTSGSSANLYSWMYVSGRGNPSTPESVSELNHNHFLSPELQD
                     KLDVMVSIYSCARNNNELEEIFQELSAFVSGLMDKRNSVFEVRNENTDEVVGALRAGM
                     TIEDRDSYIRDLFFLHSLKVKIEESRQGKEDSKCKVYNLLCPHHSSELYGDLRAMKCL
                     VEGCSDDFNPFDIIRVPDLTYNKGSLQCG"
     gene            complement(1562..>1670)
                     /locus_tag="ACHJWA_004992"
     CDS             complement(1562..>1670)
                     /locus_tag="ACHJWA_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector protein NleH"
                     /protein_id="HFO4311396.1"
                     /translation="ISSYNVSDISWSEHQVMQSYHGGKLDLISVVLSKI"
BASE COUNT          518 a          376 c          315 g          461 t
ORIGIN      
        1 aaaaacaact ttttatcttt ttgtttcgct caaattagta taaaaagccg aacaacaaac
       61 aataaaaaac cattaacatc aatagattat gatggcttca atctaaataa taggctatat
      121 aatactgcaa gacacaacac atgctgtcac tatgagtcaa ctatttagat gatattagtg
      181 acctgtttca gagcattagc gcaaggcgat tctttgtctt cttgcactaa ttttttatca
      241 taaaaatgtt cctagcactg ggcatcaata tcgcaggtca gaaagagctc ctggggatgc
      301 ggctggccga aaatgaaggg gcgaatttct ggttcaatgt gctgactgaa ctgaaaaacc
      361 gcggtctgaa cgatatcctc atcgcctgtg tgtatggcct gaaagaattc ccggaggccc
      421 gcatccagtt atgcatcgtg catatggtgc gcaacagcat gcgcttcgtg tcatggaagg
      481 aatacaaagc cgtcactcgc gacctgaaag cgattagcct cccacagaag aggcaggcca
      541 gcaggcactg gaagcgtttg ctgcggcctg ggactgccgc tatccgcaga taagccggtg
      601 ctagctgtca aactggacta acttggcgac gtttttcgct tatccggcag atatccgcaa
      661 agtgatctac acaacgaacg ccatcgagtc gctgaacagt gtgatccggc atgccatcag
      721 gaaacgcaag gtgttcccga cggacgagtc agtaaaaaaa gtggtgtggc tggcaatcca
      781 ggccgcgtca cagaaatgga caatgccgtt aagggactgg cgaatggcaa tgagccgctt
      841 tattatcgag tttggtgacc gtcttgacgg tcacttttaa gaaaaggcat ttacacagaa
      901 tcctaaacag gctcacaggc ctctaatcat ccacattgta aagatccttt gttgtaagta
      961 agatctggta ccctaataat atcaaaagga ttaaaatcat cactgcatcc ttccacgagg
     1021 catttcattg ctcgtagatc accatatagc tctgaagagt gatgcggaca tagcagatta
     1081 taaactttac atttcgaatc ttctttgcct tgtctacttt cctcaatttt tactttcaat
     1141 gaatgcagaa aaaaaagatc cctgatataa ctatccctgt cctctatcgt cattcccgcc
     1201 ctcagcgctc cgacaacctc atcagtattt tcatttctca cctcaaatac actatttctc
     1261 ttatccatca gcccacttac aaaagcactt agctcttgaa aaatttcctc aagctcatta
     1321 ttatttctgg cacatgaata tatagagacc ataacatcaa gtttatcttg taattcagga
     1381 gaaagaaagt gattatgatt aagctcactt actgattccg gagtcgaagg gttacctctt
     1441 cctgatacat acatccatga ataaagattt gctgaagaac cacttgttgg taacatatca
     1501 aaaccccctt aacaaataaa tccataaata ttattgcaac taatatatct ggataaaaat
     1561 attatatctt acttaatact acactaataa gatccagctt tcctccgtga taagattgca
     1621 tgacttgatg ttcactccat gaaatatcag aaacattata agatgatata
//
LOCUS       DAZFAB010000275         1641 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZFAB010000275 DAZFAB010000000
VERSION     DAZFAB010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.278"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..245)
                     /locus_tag="ACHJWA_004993"
     CDS             complement(<1..245)
                     /locus_tag="ACHJWA_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO4311397.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLREIDLTVQGGERYFFCNELNE
                     KGEAVTWQGRQYQVYPIDGSGFEMNGKGSSARPSLTVS"
     gene            complement(245..574)
                     /locus_tag="ACHJWA_004994"
     CDS             complement(245..574)
                     /locus_tag="ACHJWA_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4311398.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWTPPYAWRQIKVTCAAWSSRVRMLRVEFSA
                     EFKQVVN"
     gene            complement(571..1641)
                     /locus_tag="ACHJWA_004995"
                     /pseudo
     CDS             complement(571..1641)
                     /locus_tag="ACHJWA_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
BASE COUNT          314 a          594 c          370 g          363 t
ORIGIN      
        1 gacaccgtca gcgacggtct ggcactgctg cccttcccgt tcatctcaaa accgctgccg
       61 tcaatcgggt atacctgata ttgccgcccc tgccaggtga ccgcctcccc tttttcattc
      121 agctcattac agaaaaaata ccgctcaccg ccctgcaccg tcaggtcaat ttccctgagc
      181 accacccgcg gtgactgctc tgatttaacc gactcgttca gactttcttc gtgaatatcc
      241 tgcatcagtt caccacctgc ttaaactcgg cactgaattc aacccgcagc atgcgaaccc
      301 gtgatgacca ggcggcacag gtcaccttta tctgccgcca ggcataaggc ggtgtccaca
      361 gaaacgcctt ccagccaccg tgctctgcca ggaatgcctc cagatgtcgg gcctcctccc
      421 gggtcacgga aagcgtcaca cggtatgttt tcaggtcagc attcagcccc gccgccatac
      481 gctgcgaata cccgtcacca aaacgcactt cacgcaccga tggctgcgag ttcacctcca
      541 tatccggctt cactttccag cgaaatgttt tcatcgcctg cctccggaaa agacgccgcc
      601 atcacgcatc tgcgcctgaa tctcatcctg cgcccccttg cgggccatgt catacaccgc
      661 cttcatcagc tgcggccccg cctgtccgtt ggagccgtcg ttctgaatca ccacgtgatt
      721 gttctgatta aaattaatgc cttccgtccg ccgcatctgc gccggacttc cggcaccacc
      781 cacataacca ccttccgcat agccccgcat caggcggtac aggttgccga cgccaatccg
      841 gctggttgct tccttcgtga agacaaactc cccgcgatga acaattcccg caggttcata
      901 tttacccccc gtccccgtaa atcccccggt cgcgaaatgg aagttcgccg ccgcagcctg
      961 aatggccgtc cccgtggagg cagacgcacc accaccgaaa gcaccgccaa tggcgctgcc
     1021 gatactcccg actatcccca ccatcgcctg cttcagaaaa atctctgtca gcatggacag
     1081 caccgaacgg gtgaatcccc gccagtctgc ctctgcaccg gtcagcatcg ccgccatatt
     1141 ctgtgcaata ccgtcaaagg tctgcgtggc agcactttta acctgcgaaa aactgtccgt
     1201 cgcactttcc gcccactcgc cccagccgga cttcagcccg gccatccagc ttccacgaag
     1261 ctgctcctcc gcagaccagg tgttcttcag tgcagatgtg gccttcgcca gcgcatccgg
     1321 attatcaccg tacacgtcac gaaggcgctg ctcttccgac tcccgctgcg cctgacggtc
     1381 ggtgaggccc gcgcctgggc actgattgcc gcctgcttcg cgccctgctg ctgctcaaac
     1441 cgcgcagcct gctgtgccag ctcattcagc cgtttctggt gttcaacttt gtctcccagc
     1501 tcagccagct ggcgtttgta ctccagcgtc tcgtctttat gcgccagcag ggatttttcc
     1561 tgctcagata actgccgttt cgtggcagcc tctttcagga ccgcatactg attttccgct
     1621 ttccataaat cgcgacgctg c
//
LOCUS       DAZFAB010000276         1639 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZFAB010000276 DAZFAB010000000
VERSION     DAZFAB010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.279"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..397
                     /locus_tag="ACHJWA_004996"
     CDS             <1..397
                     /locus_tag="ACHJWA_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311399.1"
                     /translation="NLMERYLLLLDRFVDKLAESGFSEQRIIEQSYLFCAGFYIKYQS
                     GIEKMTFSNREVVLTFLLLSYYSHINKLDDDLINKERMKHVCSSLINFIVSNGSRTEK
                     VYANEKRKYEASTLKRELSKKDKRKRYRL"
     gene            793..>1639
                     /locus_tag="ACHJWA_004997"
     CDS             793..>1639
                     /locus_tag="ACHJWA_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311400.1"
                     /translation="MTIAKDSSTDEIIHGNDLRGMDDFYIKTTSFECPYEPCKIKATP
                     CSFTMKHVNQSYFRYGDKHKDGCGIHDPRYKNNHTSNDERKHNSPPAPVISLLKIDVK
                     PRGGVKNARNIKNENHKDEKKGNEHPVSSSSIKPVVDYYINNSNHNEQLSIPPYGTRS
                     YKDTFQLIFYKNNIRYYKPAIYYGVAQSNIRLHEDSDKHCITFLARDKKTQKPFTLEI
                     DVSDWNKSQKDVFWKEYEKQRKEADRYYKGLKDKRNAKKYLTVFFFGMPDENDKFLFK
                     TNHFKL"
BASE COUNT          593 a          245 c          288 g          513 t
ORIGIN      
        1 taacttaatg gaaaggtatc ttcttctgct tgatcgattc gttgataaac tcgctgaatc
       61 aggtttctca gagcaacgaa ttatcgaaca atcatatctt ttttgtgcag gattttatat
      121 caaataccag agtggtatag aaaaaatgac cttctccaac agggaggttg ttttaacctt
      181 tctgctactt tcatattata gtcatataaa taaactggat gatgatttga tcaacaaaga
      241 gcgtatgaag catgtatgtt cttcattgat taactttata gtcagtaatg gtagccgaac
      301 agagaaagtc tatgctaatg agaaaaggaa atatgaggct tcaactctaa aaagagaatt
      361 atcgaaaaag gataaaagga aaagatatag gttgtagtgt ttgtatgatt ttcattagac
      421 ttacttaatc tatagtctaa attttaatat tagattcgta aatctataat tccatgaatc
      481 taattaatgc ttaaaagcat cctctaagat gcttttactg ttaccatgca tacataccca
      541 gaacctttaa caaacgataa tagaggggag ttgtgtataa caactccctg accttactaa
      601 ggttagcgat gttattcagc atcattctgt caaagattta atttctttga aaaatactaa
      661 ttctacccct taagttaata acttctgctt gatcgtgtat tttggtaagt tggagtacaa
      721 ttttttgatt ggatcgtcaa taagaaaaag ctagaatcct tagaaggaca tagattttca
      781 tagggttaag atatgacgat tgcgaaggat agttcgactg atgaaatcat tcatggaaat
      841 gatctccgtg gaatggatga tttttacatt aagacaacga gttttgaatg cccatacgag
      901 ccttgtaaaa ttaaagccac accttgtagt tttactatga aacatgttaa tcaatcatat
      961 ttcagatatg gcgataaaca taaggatgga tgtggtattc atgatccaag atataaaaac
     1021 aatcatactt caaatgacga aagaaaacat aattcgccac ctgctccggt tatatcactt
     1081 ttaaaaattg atgtgaaacc aagaggtggt gttaaaaatg ccagaaatat taaaaatgaa
     1141 aatcacaagg atgaaaaaaa aggtaatgaa catcctgtat cttcatcaag tattaagcca
     1201 gtcgttgact attacataaa taatagcaat cataatgaac agctatcaat ccctccatat
     1261 ggcacgcgat cttataaaga tacatttcaa cttatttttt acaaaaacaa catacgttat
     1321 tacaaacctg ctatttatta tggtgtagcc cagtctaata ttcgtctgca tgaagattca
     1381 gataaacatt gtattacttt cctcgcaagg gataagaaaa cccaaaaacc atttacgctt
     1441 gaaattgatg tttctgactg gaataaaagt cagaaggatg tgttttggaa ggaatatgaa
     1501 aagcaaagga aggaagctga taggtattat aaggggctta aagataaaag gaatgctaaa
     1561 aaatacttga cggttttctt ttttgggatg ccggatgaaa atgataagtt tctatttaag
     1621 acaaaccact ttaaattag
//
LOCUS       DAZFAB010000277         1634 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZFAB010000277 DAZFAB010000000
VERSION     DAZFAB010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.280"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..291
                     /locus_tag="ACHJWA_004998"
     CDS             <1..291
                     /locus_tag="ACHJWA_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO4311401.1"
                     /translation="GLYPDAESYPWKSNAHYWLVTNLYQNMRANALTDAELRRKAADE
                     LTCMTARINRGEAIPEPVKQLPVMGGRPLNRVQALAKIAEIKAKFGLKGASV"
     gene            288..572
                     /locus_tag="ACHJWA_004999"
     CDS             288..572
                     /locus_tag="ACHJWA_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HFO4311402.1"
                     /translation="MTGKEAIIHYLGTHNSFCAPDVAALTGATVTSINQAAAKMARAG
                     LLVIEGKVWRTVYYRFATKEEREGKVSTNMIFKECRQSAAMKRVLLVYRT"
     gene            749..997
                     /locus_tag="ACHJWA_005000"
     CDS             749..997
                     /locus_tag="ACHJWA_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311403.1"
                     /translation="MHELFVLVLSTCASLSNMSGCSVEVVNVNTTKEPVNVFYSRKDC
                     EESMKNIMLNHALYHEVSGREPYMAKCEQVFVSKSLMK"
     gene            1041..1322
                     /locus_tag="ACHJWA_005001"
     CDS             1041..1322
                     /locus_tag="ACHJWA_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311404.1"
                     /translation="MEDYLVFGLGYEGDIKNDEAGLDKISVVTKSVVRSTNSNEPVVY
                     QVTEFKEFNVVRHQAFDSEYYNIAFDVLPSRVRIDAAIRKYHPKKSSSA"
     gene            1413..1514
                     /locus_tag="ACHJWA_005002"
     CDS             1413..1514
                     /locus_tag="ACHJWA_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311405.1"
                     /translation="MRTYNPNSLLPSLMQKCTCGSLHPTFDLCGGEA"
     gene            1511..>1634
                     /locus_tag="ACHJWA_005003"
     CDS             1511..>1634
                     /locus_tag="ACHJWA_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311406.1"
                     /translation="MNLPQDGIKLHRGNFTAIGQQIQPYLENGKCFRMVLKPWRE"
BASE COUNT          485 a          347 c          397 g          405 t
ORIGIN      
        1 ggcctgtatc cggatgcaga gtcttatccg tggaaatcga acgcgcacta ctggctggtt
       61 accaacctgt accagaacat gcgggccaat gcgctgactg acgcggaatt acgacgcaag
      121 gctgccgatg aactgacctg tatgacagcg cgaattaacc gtggtgaggc gatacctgaa
      181 ccagtaaaac aacttcctgt catgggcggc agacctctaa atcgtgttca ggcgctggcg
      241 aagatcgcag aaattaaagc taagttcgga ctgaaaggag caagtgtatg acgggcaaag
      301 aggcaattat tcattacctg gggacgcata atagcttctg tgcgccggac gttgccgcgc
      361 taacaggcgc aacagtaacc agcataaatc aggccgcagc taaaatggca cgggcaggtc
      421 ttctggttat cgaaggtaag gtctggcgaa cggtgtatta ccggtttgct accaaggaag
      481 aacgggaagg aaaggtgagc acgaatatga tttttaagga gtgtcgccag agtgccgcga
      541 tgaaacgggt attgttggta tacagaactt agcatcgaag gcctattgtt ttcgacagga
      601 ggccatcgaa atgacactat ggtaccgtct ttacccaccg ccttctgcta agtttaggaa
      661 tacaagatgt tgctgccgtt aaaaaatagt cgtaatttct attagaattt gggcaatata
      721 accagcacat agataaggga gtcgttttat gcacgaacta tttgtgctgg ttttgagcac
      781 ctgtgctagc ctcagcaata tgtcaggttg ttcagtggaa gtagtgaatg tcaacaccac
      841 aaaagagccg gtaaacgtct tttatagcag gaaagactgc gaagaaagca tgaaaaacat
      901 tatgctaaat catgctctat accatgaagt atcaggcaga gagccatata tggcaaagtg
      961 cgaacaagta tttgtctcaa aaagtttaat gaaataattg gccaaaaacg acatataaca
     1021 aactcttata ggagcttaac gtggaagatt acttagtttt tggtttaggt tatgaaggcg
     1081 atataaagaa cgatgaagca gggcttgata agataagcgt ggtaacgaaa tcggtagtgc
     1141 gttctaccaa ttccaatgaa ccagtggttt accaagtgac tgagtttaaa gaatttaatg
     1201 ttgtgagaca tcaagcgttt gatagtgaat actacaacat agcattcgat gtcttaccat
     1261 cccgcgtccg tattgatgct gcaatccgta aatatcaccc caagaaatcc tcctcagctt
     1321 agtaagcttt gattttccat tatcaaccag caataataat gtcctcggag cctgaacaac
     1381 tccggtgact tctgcgctaa acggggacgt ttatgcgcac atacaatcca aactctcttc
     1441 tcccttcact gatgcagaaa tgcacctgtg gttctttgca tccaacgttt gacctctgcg
     1501 gaggtgaagc gtgaacctcc cacaagacgg tatcaaattg catcgcggta acttcactgc
     1561 tatcggccag cagatccagc cttatctgga gaacggaaaa tgctttcgca tggtgcttaa
     1621 accgtggcgt gaga
//
LOCUS       DAZFAB010000278         1630 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZFAB010000278 DAZFAB010000000
VERSION     DAZFAB010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.281"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..1199
                     /gene="escC"
                     /locus_tag="ACHJWA_005004"
     CDS             <1..1199
                     /gene="escC"
                     /locus_tag="ACHJWA_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE outer membrane
                     ring protein EscC"
                     /protein_id="HFO4311407.1"
                     /translation="YLDIDSLLKYLSDTISVNKNSCNVRKITTFNSIEVRGVPECIKY
                     ITSLSESLDKEAQSKAKNKDVVKVFKLNYASATDITYKYRDQNVVVPGVVSILKTMAS
                     NGSLPSTGKGAVERSGNLFDNSVTISADPRLNAVVVKDREITMDIYQQLISELDIEQR
                     QIEISVSIIDVDANDLQQLGVNWSGTLNAGQGTIAFNSSTAQANISSSVISNASNFMI
                     RVNALQQNSKAKILSQPSIITLNNMQAILDKNVTFYTKVSGEKVASLESITSGTLLRV
                     TPRILDDSSNSLTGKRRERVRLLLDIQDGNQSTNQSNAQDASSTLPEVQNSEMTTEAT
                     LSAGESLLLGGFIQDKESSSKDGIPLLSDIPVIGSLFSSTVKQKHSVVRLFLIKATPI
                     KSASSE"
     gene            1213..>1630
                     /gene="cesD"
                     /locus_tag="ACHJWA_005005"
     CDS             1213..>1630
                     /gene="cesD"
                     /locus_tag="ACHJWA_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone CesD"
                     /protein_id="HFO4311408.1"
                     /translation="MSRKFSSLEDIYDFYQDGGTLASLTNLTQQDLNDLHSYAYTAYQ
                     SGDVITARNLFHLLTYLEHWNYDYTLSLGLCHQRLSNHEDAQLCFARCATLVMQDPRA
                     SYYSGISYLLVGNKKMAKKAFKACLMWCNEKEKYTTY"
BASE COUNT          542 a          306 c          319 g          463 t
ORIGIN      
        1 catatctgga tattgactca ctgctaaaat atctgagtga tacaatctca gtaaacaaaa
       61 actcttgcaa tgtgagaaaa attacgacat ttaactcgat tgaagttcgg ggtgttcctg
      121 aatgtataaa atatattaca tctttatcag aaagtcttga taaagaggca cagtctaaag
      181 ccaaaaataa agatgttgtt aaggtcttta aactgaacta tgcgtcagcg acagatataa
      241 catacaaata ccgtgaccaa aatgttgtcg tcccaggtgt tgtctccatt ctgaagacaa
      301 tggcaagtaa tggttcactt cccagtaccg gtaaaggtgc cgtcgagcga agcggtaacc
      361 tgtttgataa cagtgtaacg atttccgctg atccacgtct taatgcagtt gttgtaaaag
      421 accgcgaaat aacgatggat atctatcagc aattaatttc tgagcttgat attgaacaaa
      481 ggcaaattga aatttctgtc tcaattattg atgttgatgc aaacgatctt cagcagctcg
      541 gcgtaaactg gtccggtacg ttaaatgcag gacaaggtac gattgccttt aatagctcta
      601 ccgcacaggc taatatcagc tcttctgtga taagtaatgc cagtaatttt atgattaggg
      661 tgaatgcgtt acaacagaac tcgaaagcta aaatactctc tcagccttca attataacct
      721 taaataatat gcaggcgatt cttgataaaa acgtaacgtt ttatacaaaa gtctccggag
      781 aaaaggtggc gtctctggaa agtattacaa gcggaacatt gctgagagtg acaccacgta
      841 tacttgatga cagctcgaat agccttaccg gaaaacgtcg ggaacgagtt cgtttgttac
      901 ttgatattca ggatggcaat caatcgacta accaaagcaa tgctcaggat gccagcagca
      961 ccttacctga agtacaaaat tcagaaatga caacagaagc gacattaagt gcaggagaaa
     1021 gtttattact tggcggattt atccaagata aagaaagttc aagtaaagat ggtataccgc
     1081 tgttaagcga cattcctgtt attggtagtt tattctccag tacggtaaaa caaaaacata
     1141 gtgttgttcg actcttttta attaaagcaa ccccgataaa atctgcatct agcgaataat
     1201 aaatgaggct ttatgagcag gaaatttagc tctctagagg atatttatga tttctaccag
     1261 gatggtggca cattagcgtc attaacaaat ctgacacaac aagatctcaa tgaccttcat
     1321 tcttatgcct atacagcata tcaatctggt gatgttataa ccgcaagaaa tctattccat
     1381 ttgctcacat atctggaaca ctggaattat gactacacct tatctctggg cttatgtcat
     1441 cagcgtttat caaatcatga agatgcacaa ctgtgtttcg cacgctgtgc aactttagtt
     1501 atgcaagatc ccagggcatc ttattattct ggaattagct acttactcgt cggaaataag
     1561 aaaatggcca agaaagcctt taaggcttgt ttaatgtggt gtaatgaaaa agaaaaatac
     1621 actacatata
//
LOCUS       DAZFAB010000279         1626 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZFAB010000279 DAZFAB010000000
VERSION     DAZFAB010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.282"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            21..791
                     /locus_tag="ACHJWA_005006"
     CDS             21..791
                     /locus_tag="ACHJWA_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO4311409.1"
                     /translation="MGTVLHALKAMGKANSVELAARLDISREEVLNELWELKKNGVVD
                     KTGHTWFLAGEGESGVTEEQPAQSEVPDVLTGEVEQKVTADMMIEFICQDGAKTCEEL
                     ADKFGVSIRKVASTLAVVTATGSLARVNQNGKFRYCIPGADLPAEPEAASVTETDGKA
                     FPQPTGVALPVREAETQEEIKTESVAVTVQSQPLFTRKHPDGLILPSLHVANRELRRA
                     KGQVQKWERVCAALRELNKHRDIVRQIVDSSGRIVSEK"
     gene            807..1202
                     /locus_tag="ACHJWA_005007"
     CDS             807..1202
                     /locus_tag="ACHJWA_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO4311410.1"
                     /translation="MAKPFTHEQREELKARIIGLVRKNERMTISQLERATGAGWHSVR
                     RCLVDVLACGDLYMPGKYGVFTSEQVYRVWRKAAEKTTDQTLIRKLPDGEIRRYDRQQ
                     NIICGECRKSEVMLRVLAFYQGNFQEAVL"
     gene            1259..1615
                     /locus_tag="ACHJWA_005008"
     CDS             1259..1615
                     /locus_tag="ACHJWA_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311411.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERAYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRILRQEERERRRNIEKLIQIE
                     RNVKARIRRVTKSRTE"
BASE COUNT          466 a          324 c          476 g          360 t
ORIGIN      
        1 tttatccgga ggaaatttta atgggaaccg tattgcatgc actgaaagcg atgggtaaag
       61 ccaattctgt tgaactggcg gcgcggcttg atatcagccg tgaagaagtt ctcaacgaac
      121 tgtgggaact caaaaaaaat ggcgttgttg ataaaacggg tcacacctgg tttctggctg
      181 gtgaaggtga atccggggta accgaagaac agccagcaca gtctgaagta ccggatgtgc
      241 tgaccgggga ggtcgaacaa aaagttactg cggacatgat gattgagttt atctgtcagg
      301 atggggctaa aacgtgtgag gaactggcgg ataagttcgg tgttagcatt cgcaaggttg
      361 cttccacgtt ggcggtcgta acagcaacgg ggagcctggc acgcgtaaat cagaacggta
      421 aatttcgtta ctgcataccg ggcgctgatt taccggcaga gccggaagct gcatcggtaa
      481 cggaaaccga tggtaaagcc tttcctcagc caacaggtgt tgcgttacca gtccgggaag
      541 cggaaacaca ggaagaaata aaaactgaaa gtgtggcggt cacagtgcag tcacagccgt
      601 tgttcaccag aaaacatccg gatggtctga ttttaccatc gctgcatgtg gctaaccgcg
      661 agctgcgccg ggcaaaaggt caggttcaga agtgggagcg tgtctgcgcc gcgctgcggg
      721 agctgaacaa gcaccgggat attgttcggc agattgtcga ttcatccggt cgtattgtgt
      781 cggaaaagtg attgccggag gcgcttatgg cgaaaccttt tacacacgaa cagcgtgaag
      841 aactgaaggc ccgaattatc gggctggtac gcaaaaatga acgcatgacg atatcacaac
      901 tggagagagc gacgggagca ggctggcatt cagtcagacg ttgccttgtg gatgtactgg
      961 cttgcggcga tttatacatg cccggtaaat acggtgtttt tacatcagaa caggtgtatc
     1021 gcgtatggcg taaggcagcg gagaaaacaa ccgaccagac attgattcga aagttaccag
     1081 acggagaaat acgccgctac gacagacaac agaacataat ctgtggcgag tgccggaaga
     1141 gtgaagttat gctgcgtgta ctggcgttct atcagggcaa ttttcaggag gcggtattgt
     1201 gagtgaatta gctatcaggc ttcaattgtc gctggcattc acatcaaagg agaagagcat
     1261 ggaaataaaa ccagaagatg agttaagcaa tatcgtttta tttccggtaa aagaggatga
     1321 cccacgtaat caggttaatt ttctttatga gccatcggaa agagcatatt gtcatcacgc
     1381 ctctgttcgg gttgacgaaa aagagcgtca ggtccgctgt aaaatctgcg gtgcagttgt
     1441 ggagccattt gactggatgc tctctgtggc gaaaagagaa accagactgg cagatgatgt
     1501 aaggatcttg cgccaggagg aacgggaaag gcggagaaat atagaaaagc taattcagat
     1561 tgagcgtaac gtgaaagcgc ggatacgcag ggtgacaaaa tccagaactg aataattaaa
     1621 tttagc
//
LOCUS       DAZFAB010000280         1619 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZFAB010000280 DAZFAB010000000
VERSION     DAZFAB010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.283"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            132..503
                     /gene="ler"
                     /locus_tag="ACHJWA_005009"
     CDS             132..503
                     /gene="ler"
                     /locus_tag="ACHJWA_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE master regulator
                     Ler"
                     /protein_id="HFO4311412.1"
                     /translation="MNMENNSHTTSPYIQLIEQIAVLQQEAKRLREQEVQSVIESIQK
                     QITYYNITLQELGYTNVPDDGLARRNSSKGVYYRNEEGQTWSGVGRQPRWLKEALLNG
                     MKKEDFLVKDTEEEIIPLKNI"
     gene            518..736
                     /gene="escE"
                     /locus_tag="ACHJWA_005010"
     CDS             518..736
                     /gene="escE"
                     /locus_tag="ACHJWA_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YscE family type III secretion system
                     co-chaperone EscE"
                     /protein_id="HFO4311413.1"
                     /translation="MITITELEDEIIKNKEAANVFIEKINDKKNEIHEKMKHPLDKVT
                     YNEAKELLIACDAAIRTIEIMRIRINNK"
     gene            749..1063
                     /gene="cesAB"
                     /locus_tag="ACHJWA_005011"
     CDS             749..1063
                     /gene="cesAB"
                     /locus_tag="ACHJWA_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesAB"
                     /protein_id="HFO4311414.1"
                     /translation="MSQTRNKELLDKKIRSEIEAIKKIIAEFDVVKESVNELSEKAKT
                     DPQAAEKLNKLIEGYTYGEERKLYDSALSKIEKLIETLSPARSKSQSTMNQRNRNNRK
                     IV"
     gene            1060..>1619
                     /locus_tag="ACHJWA_005012"
     CDS             1060..>1619
                     /locus_tag="ACHJWA_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system domain-containing
                     protein"
                     /protein_id="HFO4311415.1"
                     /translation="MTIFNKIDYNYYKLINYPIMMVHDEWLGDLTGVNQVSFRRLRET
                     SSTRNQLNKILRQEIHDKISGVELSDINKEGFLYQSIGKIRLLALSSALFDIQCPDYI
                     FSRLYRETLIREIGYQNVKQLSFYWQGGQCKPEYGEERFCAELIKYGAGNLEWLFADN
                     PLWTIVKYLLPKSGEIKPTHINDLFL"
BASE COUNT          636 a          222 c          325 g          436 t
ORIGIN      
        1 tgaaatagat gtgtcctaat ttgatagata aacgttatct cacataattt atatcatttg
       61 attaattgtt ggtccttcct gataaggtcg ctaatagctt aaaatattaa agcatgcgga
      121 gattatttat tatgaatatg gaaaataatt cacatacaac aagtccatac attcagctta
      181 tagagcaaat tgcagttcta cagcaggaag caaagcgact gcgagagcag gaagttcaaa
      241 gtgtaattga gtcgattcag aagcagatta cttattacaa tataacctta caagagctgg
      301 gatatactaa tgtgcctgat gatggactcg ctcgccggaa ctcatcgaaa ggtgtttact
      361 accgcaatga agaagggcag acctggtcgg gcgtaggccg acagccacgc tggcttaaag
      421 aagcactgtt gaatggaatg aagaaagaag attttcttgt gaaggacact gaagaagaaa
      481 taataccgct gaaaaatatt taacatgaaa taattaaatg ataacgataa ctgagctgga
      541 agatgaaata ataaaaaata aagaagccgc aaatgttttt attgaaaaaa taaacgacaa
      601 aaagaacgaa atccatgaaa aaatgaaaca ccctttggat aaagttacct acaatgaagc
      661 aaaggaactg ctcattgcgt gtgatgcggc aattaggact atagagataa tgcgaatcag
      721 gattaataat aaatagagga tgagtattgt gagccaaaca agaaataaag aactattaga
      781 taaaaaaata agatcagaaa ttgaggcgat aaaaaaaata atcgccgaat ttgatgtcgt
      841 aaaagaaagc gttaatgagt taagtgaaaa agcaaaaaca gatcctcagg cggcagaaaa
      901 attaaacaaa ttaattgagg ggtacacata tggagaagag cgaaaattat atgattctgc
      961 cctaagtaag attgaaaaac taatagagac actgagtcca gcaagatcta aaagccaatc
     1021 aacaatgaat caacggaata gaaataatag aaaaatagta tgacaatttt taataaaata
     1081 gactataact attataaatt aataaattat ccaatcatga tggttcatga tgaatggcta
     1141 ggtgatttaa ccggtgtcaa tcaggtcagt tttcggcgtt taagagaaac tagttctact
     1201 cgtaatcaac tcaataagat attgcgtcag gaaatacatg ataaaatttc tggtgtggaa
     1261 ttatctgaca taaataagga aggtttttta tatcagtcaa ttgggaaaat aagattgctt
     1321 gctctaagta gtgcattatt tgacattcaa tgccctgatt atattttctc acgattatat
     1381 cgagaaactc tgattagaga gataggatat caaaatgtga agcagttatc attctattgg
     1441 cagggagggc aatgtaaacc agaatatggt gaagaacggt tttgtgctga gttaataaaa
     1501 tacggtgcag gaaatttgga gtggctattt gctgataatc ctttatggac catagtcaaa
     1561 tatcttttgc cgaaaagtgg agaaataaaa ccaactcata ttaatgattt atttcttaa
//
LOCUS       DAZFAB010000281         1613 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZFAB010000281 DAZFAB010000000
VERSION     DAZFAB010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.284"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            123..341
                     /locus_tag="ACHJWA_005013"
     CDS             123..341
                     /locus_tag="ACHJWA_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311416.1"
                     /translation="MTNPIGINNLSQSSNIANATGDEVVSLDKHINTSATDKVQIQAF
                     IVSTWMASFQNDMYSEDNPISPYHKIEW"
     gene            426..929
                     /locus_tag="ACHJWA_005014"
     CDS             426..929
                     /locus_tag="ACHJWA_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFO4311417.1"
                     /translation="MRSPKVKFLTIFTLSILITKMSFASSACFNEAGTMFRIEPNLIK
                     AIALVESNLKKDSIGKNRDKKNNIKSFDYGLMQINQMHIPMLKKRGIIKDERDLLDNP
                     CLNIKIGTEILYKHFSRCGMTWQCLGTYNAGFAMDNQKKRLQYAKKIYIVYTRLNELD
                     NRKALAK"
     gene            complement(948..1580)
                     /locus_tag="ACHJWA_005015"
     CDS             complement(948..1580)
                     /locus_tag="ACHJWA_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFO4311418.1"
                     /translation="MGKIKIVVSDQQPFMIDGIIGFLGHYPDLYEVVGGYKDLKKAIA
                     ECNKSAAQIFILGEFAGGMMGAELVKWVKSHKIDAHIITFVAKMPYIDSIKLLEAGAK
                     GCVWKTSHPAKLNRAIDSISNGYTYFDSVHMDCEKISSRYSSDNQLTNRESEILQLIA
                     DGKTNKEIANFLQLSRKTVETHRLNIMKKLDVHSGIELIKTALRMGVCTI"
BASE COUNT          554 a          292 c          257 g          510 t
ORIGIN      
        1 tatttaaaaa acaaagatca aaatatctaa gatatttacc ttattgcaag atatttaaaa
       61 tgctctagag ttaaaagtat gatgtaaaga ccattggcct ggacaattaa aaagggagct
      121 ttatgactaa tccaatcggt atcaataatt tatctcaaag ttcaaatata gcgaatgcaa
      181 cgggtgatga agtcgtttca ttagataaac acatcaacac cagtgctact gataaagttc
      241 aaatacaagc atttatagtt tcaacatgga tggcgtcttt tcaaaatgat atgtattcag
      301 aagataatcc tatctcacct taccataaaa ttgaatggta gacgattgac caaataaatt
      361 attaacgaga tactgtttat aatagtatct cgttcctttt atacaatcct gactcataaa
      421 gatatatgcg ctcaccaaaa gttaaattct taaccatctt cacgctttct atattaatta
      481 caaaaatgtc tttcgccagt agcgcttgct ttaatgaagc aggaacaatg ttcagaatcg
      541 aaccaaattt gattaaagct atcgctcttg ttgaaagcaa tctaaaaaag gatagcatag
      601 ggaagaacag agataagaaa aacaatataa agagttttga ttatggttta atgcagataa
      661 accagatgca cattcccatg ttaaaaaaac gtggaataat aaaagatgaa cgagatttgt
      721 tagataatcc ctgcctgaac attaaaatag gaaccgagat tttatataag catttttccc
      781 gttgtggaat gacgtggcaa tgcctgggga cttataatgc gggttttgca atggataacc
      841 aaaaaaagcg actgcaatat gcaaaaaaaa tatatattgt atataccagg cttaatgaac
      901 tagacaatcg taaggcttta gcaaaatgag cctaaagcct ctttttttta tatagtgcac
      961 acacccatac gtaaagctgt tttgatcaac tcgataccac tgtgaacatc taatttcttc
     1021 atgatattga gtctatgagt ttcgactgtt ttcctgctta actgcaagaa attagcgatt
     1081 tctttgtttg tttttccatc agctatgagt tgtaatattt cagactcacg atttgtaagt
     1141 tgattatcag aggaatatct ggaagatatt ttctcacaat ccatatgtac actatcaaaa
     1201 taagtgtagc cgttactaat cgaatcaata gcgcgattaa gtttcgccgg gtgactggtt
     1261 ttccatacgc atcctttcgc acctgcttca agcaatttta ttgaatcaat atacggcatt
     1321 tttgctacaa atgtaataat atgagcatct attttatgcg atttaaccca ttttacgagt
     1381 tcggccccca tcattccacc tgcaaattct cctaaaataa atatttgggc tgcagatttg
     1441 ttacactccg ctatagcttt cttcagatct ttatagcccc caacaacctc atataaatcg
     1501 ggataatgtc cgaggaatcc aattatccca tcaatcataa acggctgctg atctgaaact
     1561 acaattttaa tttttcccat atattcatcc tgagtgtaaa tgctttaacg cat
//
LOCUS       DAZFAB010000282         1575 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZFAB010000282 DAZFAB010000000
VERSION     DAZFAB010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.285"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..91)
                     /locus_tag="ACHJWA_005016"
     CDS             complement(<1..91)
                     /locus_tag="ACHJWA_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311419.1"
                     /translation="MCVTSNVLVMFSSFEIFDVISYKVVFIVYL"
     gene            395..801
                     /gene="ipbA"
                     /locus_tag="ACHJWA_005017"
                     /gene_synonym="fimX"
                     /pseudo
     CDS             395..801
                     /gene="ipbA"
                     /locus_tag="ACHJWA_005017"
                     /gene_synonym="fimX"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="IpbA is also known as HbiF and FimX; frameshifted;
                     incomplete; partial in the middle of a contig; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase IpbA"
     gene            1227..1575
                     /gene="hyxR"
                     /locus_tag="ACHJWA_005018"
                     /pseudo
     CDS             1227..1575
                     /gene="hyxR"
                     /locus_tag="ACHJWA_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304859.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator HyxR"
BASE COUNT          488 a          327 c          301 g          459 t
ORIGIN      
        1 taagataaac aataaaaaca accttataag aaataacatc aaatatctca aaactactaa
       61 acatcactaa aacattagac gtaacacaca tcccccacgc cctgtaattc atagctttta
      121 tatttatttt ttcacatcaa tgtagataaa aacacgcatt aaaaatggct tgtagaaaag
      181 cagcacaata aatagttcat atttagtcta ttgcgtagaa ctactattca aaaaatctta
      241 ttaatgagat aatgagatta catcgataac gttctgattg caggcatact tatctgggtg
      301 tgcctctctt ttttacaact gtacacggtg gcgtatgacg agaaaatatc tcacacagga
      361 tgaagtctac aggctgatgg atgcagccca gagcatgtcc tttcctgaaa gaaatcgctg
      421 tctaattatg atggctttca ttcacggttt tagggccagt gaacttcttg atttacgttt
      481 atccgatatt gatgcatcag gaaaacaact taatattcgg cggattaaaa atggtttctc
      541 gacaacacat cctcttcttc ctgatgaata taatttgatc aagttgtggc ttaagcagag
      601 gaagttaatt gaaaacggag ttgaaggaga ctggcttttt ctctcccgga aacgccgccc
      661 tatcagcaga caacattttt ttctatcatt cgtgaggctg gaagacgtgc aggattagct
      721 gtaaaagcac atcctcatat gttacgtcat gcctgtggtt ttgctctggc tgacaatggt
      781 gtcgataccc gactacttca ggattatctg ggtcatcgaa atatccagca tactgtcaga
      841 tacacagcca gtaatgctgc tcgatttaaa ggggtgtgga aaaaaaagcc tcgttaaaac
      901 agtaccttct gattcaaaat gtcagattcc tacgttttcc tcaattcttc cataaaaaaa
      961 cagcccattc aattcaataa aacacataac atgttgatat ttctcgctaa agtaaaataa
     1021 ccccatgatt tgaaatgatt aaccgccaag gtcataccag tgattaactt tcactcatgt
     1081 tgttaaatgc aaccaaatta aaaacaataa aaatcaaaca aattagattt atattccaag
     1141 aaaattaaat attcaggcaa ttaaacaaaa cctgacattt tgaatcaaaa tgtcaggttt
     1201 tgtagctatg gcgtggacga gtctgaatga agaagcctct ggttattatc agtgcctgtc
     1261 agtttaccag gctggcactg gagtctctca tcccagcgga caggtatatc gtgcgggtgt
     1321 acagcaacgt cactacagag gttgaattcg ttttaaagac ctcctgtggc tatctgttgg
     1381 ctgatatccc ttagcttcct tcgggggagt tagttcttct ggcttgtctt gtccgcttgc
     1441 ggagtgatgc cgggcaatgg caggtctgcc tgaccggtga ctccgactgg ttcgataatg
     1501 ctcctgctgc aagtctgtat aacggcttcc cacgcatcgg caccatgctg acagcgagtc
     1561 gttatcagaa tcaaa
//
LOCUS       DAZFAB010000283         1554 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZFAB010000283 DAZFAB010000000
VERSION     DAZFAB010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.286"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..258
                     /locus_tag="ACHJWA_005019"
     CDS             <1..258
                     /locus_tag="ACHJWA_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO4311420.1"
                     /translation="ASFVLATPTETDGALFPGRIMLANTCMWDYRGDECGYNGPAVAD
                     EFDNPTTDIRKDRCSKCMRGCELRRNVGNFGGFLSINKLSQ"
     gene            complement(275..595)
                     /locus_tag="ACHJWA_005020"
     CDS             complement(275..595)
                     /locus_tag="ACHJWA_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFO4311421.1"
                     /translation="MAEKQVKDYDKFNLRFPDGMRDAIAERAKRNGRSMNSEIVQILE
                     DALNAENTLGEIADKINSVSVPLNVDALVQLQAQVIAMQKEIQEKFREQNEKLRELLN
                     KKPT"
     gene            703..876
                     /locus_tag="ACHJWA_005021"
     CDS             703..876
                     /locus_tag="ACHJWA_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFO4311422.1"
                     /translation="MQDTLFTERKNIKLNLRLPSRLNEDLRRLAEMDCISLNSAIVRL
                     LAKGVREEVANGR"
     gene            947..>1554
                     /locus_tag="ACHJWA_005022"
     CDS             947..>1554
                     /locus_tag="ACHJWA_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001428824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HFO4311423.1"
                     /translation="MTSLAIADRTINVPFHGTNLFLVGINNEPYVPMKPVVEGMGMVW
                     AAQFVKLKQRFVKGISEIEIPSAGGKQLMTCLAFRKFAAWLSSIQPNKVRPEIRDKVI
                     QYQEECDDVLYEYWTKGHVINPRKAKKALPGKITTEQQEAIKQLVMSRGQSLPKEKQA
                     KAMITMWSSLKSHFGCSYKEISEEQFTEALSLAARVPLEGEL"
BASE COUNT          408 a          344 c          372 g          430 t
ORIGIN      
        1 gcctcgtttg tgctggccac accgaccgag acggacgggg cgctgtttcc cggtcgcatc
       61 atgctggcga acacctgtat gtgggattac cggggagatg aatgcgggta taacggtcct
      121 gcggtggcgg atgagttcga caaccccacc acggatatcc gtaaggacag atgcagcaag
      181 tgcatgcgcg ggtgtgaact gcgcaggaat gtcggcaatt ttggcggttt cctttccatt
      241 aataaacttt cgcagtaatg gattatgccc accgtcaggt gggtttttta tttagtagtt
      301 ctctcaactt ttcgttctgc tctctgaact tttcctgtat ttctttttgc atggcgataa
      361 cctgggcttg aagttgaact agcgcatcaa catttagcgg aaccgagacg ctgttaattt
      421 tatctgctat ttccccgagt gtattttctg cattcaaggc atcttccagt atctgaacaa
      481 tctctgagtt catagagcgc ccgtttcgtt tggctcgttc agctatagca tctcgcattc
      541 cgtcaggaaa acggaggttg aacttgtcgt agtcttttac ttgtttttcg gccattgcaa
      601 atctctcaaa aaaaatcatg gtgccatatt gccatacgat ttcaatggtg gcattatggc
      661 cttcatggcg tcactttggc accaaataaa ggagattaga taatgcaaga tacacttttc
      721 actgagcgca aaaatatcaa actcaacctt cgccttccat cacggctgaa tgaagacctt
      781 cgccgcctgg cggaaatgga ctgtatatct ctgaactctg caattgttcg tttgctggca
      841 aaaggtgtta gggaagaggt ggcgaatggt cgctaaaaac agcgaagcct caatggctgc
      901 aaccattgag gcttctaaat taccagttaa ccacgagaaa actgatatga ctagtttagc
      961 aattgcagat cgcacaatca atgttccatt ccacggaaca aatctctttt tggttggaat
     1021 taacaatgag ccttatgttc ctatgaagcc tgttgttgaa ggtatgggga tggtttgggc
     1081 tgctcaattt gttaagttaa aacagaggtt tgtcaaaggt atttcggaaa tcgaaatacc
     1141 ttctgctggc ggtaaacagt taatgacatg tcttgccttt cgtaaatttg cggcttggct
     1201 ttcaagcatt caaccaaaca aagtccgccc tgaaatccgc gacaaggtaa tccagtatca
     1261 ggaagagtgt gacgatgtgc tctacgagta ctggactaaa ggccatgtga ttaacccgcg
     1321 caaagctaaa aaggcattgc cggggaaaat caccactgaa cagcaggaag ccattaaaca
     1381 actcgtcatg agtcgcggtc agtctctgcc aaaagaaaaa caggcgaagg cgatgatcac
     1441 catgtggtcg tcactgaaat ctcattttgg gtgttcatac aaagaaatca gcgaggagca
     1501 gtttaccgaa gctctgtcac ttgctgctcg cgttccgctt gaaggagagt taat
//
LOCUS       DAZFAB010000284         1526 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZFAB010000284 DAZFAB010000000
VERSION     DAZFAB010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.287"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..368)
                     /locus_tag="ACHJWA_005023"
     CDS             complement(<1..368)
                     /locus_tag="ACHJWA_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO4311424.1"
                     /translation="MQDIPQETHHETTRLTQSAQAVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGTGFELNGKGSSARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGN"
     gene            complement(378..707)
                     /locus_tag="ACHJWA_005024"
     CDS             complement(378..707)
                     /locus_tag="ACHJWA_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4311425.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKCEHPSLKAFLERHGGVRAFQWTPPYDWKPIRVVCRKWSASVGALWVTITA
                     DFEQVVA"
     gene            complement(707..>1526)
                     /locus_tag="ACHJWA_005025"
     CDS             complement(707..>1526)
                     /locus_tag="ACHJWA_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO4311426.1"
                     /translation="QVQLSLRQKYQQQLEQLRRDSEQKGTYNTDDYRKAEQALTESLN
                     RQLNENRRYWQQLEVVQGNWKNGVLRAFQDFTVDADNTAETAEQVFSSAFSNMGNGLA
                     TFVTTGKLNFKSFTSSVLSDMAKILAQATMMKSIKGIGSVLGFDLSSLSLNANGGIYQ
                     SADLSRYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADIGGSGMA
                     MFSPQYNIEINNDGTNGQIGPAALKAVYDLGKKAAADFMQQQARDGGRLSGAYR"
BASE COUNT          339 a          476 c          330 g          381 t
ORIGIN      
        1 ctgtttccgt tgacgaagtt caccgcatcc agaaaacggg cgtaaacctt acgccggacc
       61 accgttccgc cgaccagact ctgcaggtct tccgccatcc ccgtgaccat gccgtgcagg
      121 ttagaaaccg tcagtgtcgg acgggcagaa ctgcccttgc cgttcagttc aaatcccgtc
      181 ccctgaatgg ggtatgcctg atactgccgc ccctgccagg taaccggctc acctttttcg
      241 ttctgctcat tacagaaaaa ataacgttca ccaccgacct ctgtcagatc gatttcccag
      301 agcaccgcct gggctgactg agtgaggcgt gtcgtctcat gatgtgtttc ctgtggaata
      361 tcctgcatca gagcctccta tgccacgacc tgttcaaaat ctgccgttat ggttacccac
      421 agcgccccca cgcttgccga ccatttacga caaaccaccc tgatcggttt ccagtcataa
      481 ggtggcgtcc actgaaatgc acggacgcca ccgtgccgtt ccagaaaggc ttttaaagat
      541 gggtgttcac atttacgaac acgtatcgtc acgctgtaag tcgacaactg gttattcagt
      601 cccgccgcac gacgctgttc ataaccatcg cccagcttca ctgtcaccac tttcggctct
      661 gataccacat tcatatccgg gcgcactttc cagtgaaacg tctccattac cgatatgctc
      721 cacttaaccg accaccatca cgggcctgct gttgcataaa gtccgctgcc gcttttttcc
      781 cgaggtcata aaccgccttc agggcagccg gacctatctg cccgttcgtg ccatcgttat
      841 tgatctcgat gttgtactgc ggggaaaaca tcgccatacc tgaaccccca atatccgcca
      901 caacccccag cttaccgtca gcaccacgac gcaatggcag aatggcttca ggtcccgctt
      961 cccccatcac acccgcgcct tttgcaaaag caaaaaacgt cggacggtta accaccgtgc
     1021 cactgtaacg actcaaatca gcagactgat aaatcccccc attggcattc agggaaaggc
     1081 tgctgagatc aaatcccagt acactgccaa tcccttttat cgatttcatc atggttgcct
     1141 gcgccaggat tttcgccata tctgacagca cagaagaggt gaaggatttg aaattgagtt
     1201 tgccggtagt gacaaaagtt gccaggccat ttcccatgtt gctgaaggct gacgagaaca
     1261 cctgttctgc tgtttctgcc gtattatctg catccacggt aaaatcctga aatgcacgca
     1321 ggactccgtt tttccagtta ccctgtacaa cttcaagctg ttgccagtaa cggcgattct
     1381 cattcagttg tcggttcagg ctctccgtca gcgcctgctc ggcctttctg tagtcatccg
     1441 tgttatatgt ccctttctgc tcactatccc gcctcaactg ctccagctgt tgctggtatt
     1501 tctggcgaag actcagttgt acctga
//
LOCUS       DAZFAB010000285         1491 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZFAB010000285 DAZFAB010000000
VERSION     DAZFAB010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1491
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.288"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..245
                     /locus_tag="ACHJWA_005026"
     CDS             <1..245
                     /locus_tag="ACHJWA_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="required for invasion of epithelial cells; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
                     /protein_id="HFO4311427.1"
                     /translation="IKRFIVNSFASVQYENISVVLSKRRDIIEQAPTYEISEPVFAYD
                     KAMPVSILLALISVATCWLLWKYRAILTNLVRLKIK"
     gene            261..842
                     /locus_tag="ACHJWA_005027"
     CDS             261..842
                     /locus_tag="ACHJWA_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HFO4311428.1"
                     /translation="MNLALRKIIYAPISYIHPQRVSLNNTPINNPVLRSITNEMILLQ
                     YNLSVEHFNLNSSLIYYINNWNLLPLICLLSGCHFYRERFAERGFFYKVPDVLRDYLS
                     AIPLEINEKARYKPGIANYHNIITCGFSTLLPYIRQQPLAMQQRFNLLFPDFVDHILS
                     PLPLASTLLERITFYAKKNRDELDKISCKWCCD"
     gene            784..1044
                     /locus_tag="ACHJWA_005028"
     CDS             784..1044
                     /locus_tag="ACHJWA_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001452630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311429.1"
                     /translation="MRKKIEMSLIKSPANGVVIKRKISDGLKEIVSLKEKILLETTAK
                     IQSIEEKREEKFIQGYYDGYTKGIIDVMDNFIPLISLAMLRT"
     gene            1060..1491
                     /locus_tag="ACHJWA_005029"
     CDS             1060..1491
                     /locus_tag="ACHJWA_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001701978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311430.1"
                     /translation="MINDLKSILLKSSEEVDVFIKIFESWVTKLPSISGPVNLHIPTS
                     FKDKSLEVESYFVDKSIWNVHIAYHDDKRFVFFTDQFIAEFSPQEFVDNCEQYLINNH
                     CFSPDKVNEICEQARHYLVEKMCETHSLAMNNSVLTNPEDS"
BASE COUNT          508 a          233 c          259 g          491 t
ORIGIN      
        1 acataaagag atttattgtt aatagttttg caagcgtaca atatgagaat atttctgttg
       61 tattgtcaaa acgaagagac attattgagc aagcaccaac atatgaaatt agcgaacctg
      121 tttttgctta tgataaggct atgccggttt ctatcttact tgcattaata tccgtagcga
      181 cttgctggct gttatggaag tatagggcca ttctaactaa tttagtcagg ttgaaaatta
      241 agtagacata tgagaacgtt atgaatcttg ccctaagaaa gattatatat gctccaatta
      301 gttatattca tcctcaacga gtaagcttga ataatacacc tattaacaat ccggtgctga
      361 gatctattac caatgaaatg attctcctgc agtataatct ttcagttgaa cattttaatc
      421 tgaatagttc acttatttac tacataaaca attggaattt acttccattg atctgtttac
      481 tcagtggttg tcacttttat cgagaaaggt ttgcagaacg cgggtttttt tataaggttc
      541 ctgatgtact aagggattat ttatcagcta taccgctgga aataaatgaa aaggcacgat
      601 acaaacccgg catcgctaac tatcacaata tcatcacatg tggtttttca acgttgttac
      661 cctatataag gcaacaacca ttggcaatgc agcaacgatt taatttatta tttcctgatt
      721 ttgttgacca catactatcg ccgttaccac ttgcatcaac acttttagag agaattacat
      781 tttatgcgaa aaaaaataga gatgagcttg ataaaatctc ctgcaaatgg tgttgtgatt
      841 aaaagaaaaa tttctgatgg attgaaagaa atagtttctc ttaaagaaaa aatccttctt
      901 gaaaccaccg caaaaattca gagtattgag gaaaagagag aagaaaaatt tattcaaggt
      961 tattatgatg gctatacaaa aggcattatt gatgtgatgg ataattttat acccttgata
     1021 agcttagcta tgctcagaac ttgaaaaaaa agaataaaca tgataaacga tctgaagagt
     1081 atattgttaa aatcttcaga agaagttgat gtttttatca aaatttttga aagttgggta
     1141 acaaaattac catcaatatc aggtcctgta aatctccata tacctaccag tttcaaagat
     1201 aagtctcttg aagtagagtc atattttgtc gacaagtcaa tatggaatgt ccatattgca
     1261 tatcatgatg ataagcgttt tgtgtttttt accgatcaat ttattgctga atttagtccg
     1321 caggaatttg tggataattg tgagcaatat ttaattaata atcattgttt ctcccctgac
     1381 aaggttaacg aaatctgcga acaagccagg cattatttag ttgaaaaaat gtgtgaaaca
     1441 cattctttgg ctatgaataa ttctgttttg acaaaccctg aggactcata g
//
LOCUS       DAZFAB010000286         1486 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZFAB010000286 DAZFAB010000000
VERSION     DAZFAB010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.289"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            188..445
                     /locus_tag="ACHJWA_005030"
     CDS             188..445
                     /locus_tag="ACHJWA_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311431.1"
                     /translation="MRIEELREIFSEDGLYTVRVEKGAIVSHCRIKCLQSQQRKSGAA
                     LIHFVDGLVTDGFILRANEFVTSLPSLKEAGIKAGFSAFED"
     gene            515..793
                     /locus_tag="ACHJWA_005031"
     CDS             515..793
                     /locus_tag="ACHJWA_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311432.1"
                     /translation="MAQNSRLHNSDNSAVFASRHGRRSHAFKSDWFRHAPCTEEQAEW
                     LIQNYRRRGYEFRKALSLDYRHWIIYVRLPYSERPPRPSRTFQQRIWR"
     gene            795..>1486
                     /locus_tag="ACHJWA_005032"
     CDS             795..>1486
                     /locus_tag="ACHJWA_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO4311433.1"
                     /translation="MRVLLRPVPVPELGVVVLKPGRESMQVFHNPRVLVEPEPKSMRG
                     LPSGIVPAVRQPLAEDKSLLPFFSNERVIRAVGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATIILKQR
                     TKNLAPLPHAHQ"
BASE COUNT          345 a          372 c          406 g          363 t
ORIGIN      
        1 ctgatgtgtc aggcatcctc aacgcacccg cacttaaccc gcttcggcgg gtttttgttt
       61 ttattttcaa cgcgtttgaa gttccggacg gcgccggaat agaatcaaaa atacttaagt
      121 agcgcgcagg gagaagaggg atggaccccg aacaggggag tgctatttat ctggaaggat
      181 tctgttgatg agaatcgaag aattacgtga aatttttagt gaagatggcc tctatactgt
      241 gcgcgttgag aagggcgcta ttgtcagcca ctgccgtatt aaatgtttac agtctcaaca
      301 aaggaagagt ggagctgcgt taattcattt tgtggatggg cttgtgacgg atggttttat
      361 tttgcgtgca aatgaatttg tcacatcgtt gccgtctctg aaagaagctg ggattaaggc
      421 tggtttttct gcttttgaag attagtgaat tcatctacaa ttcagcgcag ggctgaaccc
      481 ctgttgagta acactgtgcc accggagaaa gccgatggcg caaaattcca gactacacaa
      541 ttctgataat tcagccgtct ttgccagcag gcacgggcgg cgttctcatg cattcaaatc
      601 tgactggttc cggcacgccc catgcactga agaacaggcc gaatggctga ttcagaacta
      661 ccgcagacgt gggtatgagt ttaggaaagc cctcagcctc gattatcgtc actggataat
      721 ctacgtcagg ctgccgtact ccgagcgccc accgcgtccg tcccgcacat tccagcaacg
      781 catctggagg taacgtgcgg gtattacttc gacctgttcc ggtaccggaa cttggggtgg
      841 tggtccttaa accaggccgt gaatccatgc aggtattcca taaccctcga gtgcttgtgg
      901 agccggagcc gaaaagcatg cgcggtctgc cgtccggcat cgtccctgcc gttcgccagc
      961 cgctggcgga ggataaatca ttactgccgt ttttcagcaa tgagcgtgtg attcgtgctg
     1021 ttggtggcgc tggtgcactg tctgactggc tgttgcgtca tgtcaaatcc tgccagtggc
     1081 ctcatggtga ctatcatcac agtgaaaccg ttattcaccg ttatggtacc ggcgcaatgg
     1141 tgttgtgctg gcactgcgac aaccagctgc gtgaccagac atccgaatca ctcgagcaac
     1201 ttgctcatca aaacctgtca gcatggatga ttgacgtcat cggtcacgca ataagcggta
     1261 cgcaggagcg tgaattatct ctggctgaat tatcctggtg ggcggtccgc aatcaggtgg
     1321 cggacgcgct accggaagcg gtattacgtc gttcgctggg gttgcgtgcg gaaaaaatcc
     1381 gctcaatgta ccgtgaaagc gacatcgtac cgggagagca gaccgccacc atcatactga
     1441 agcagcgcac aaaaaatctt gcgccgctgc ctcacgccca ccagca
//
LOCUS       DAZFAB010000287         1484 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZFAB010000287 DAZFAB010000000
VERSION     DAZFAB010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.290"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..226
                     /locus_tag="ACHJWA_005033"
     CDS             <1..226
                     /locus_tag="ACHJWA_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008918 - lipopolysaccharide
                     3-alpha-galactosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 8 C-terminal
                     domain-containing protein"
                     /protein_id="HFO4311434.1"
                     /translation="FIHYTGITKPWHDWANYASADYFRNIYNISPWRNIPYKKAVKKH
                     EYKEKYKHLLYQKKFLDGVFTAIKYNVMKG"
     gene            235..1377
                     /locus_tag="ACHJWA_005034"
     CDS             235..1377
                     /locus_tag="ACHJWA_005034"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HFO4311435.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKRAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(1413..>1484)
                     /locus_tag="ACHJWA_005035"
     CDS             complement(1413..>1484)
                     /locus_tag="ACHJWA_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311436.1"
                     /translation="NVEQIEPNLLGVYAGLLLAMKNK"
BASE COUNT          490 a          243 c          284 g          467 t
ORIGIN      
        1 cttcatacat tatactggta taactaagcc atggcatgat tgggcgaact acgcctctgc
       61 agattatttt cgtaatattt ataatatatc accatggaga aatatacctt ataaaaaagc
      121 tgttaaaaaa catgagtaca aagaaaaata taaacacttg ctttaccaga aaaaatttct
      181 cgatggtgtt tttacagcaa ttaaatataa tgttatgaaa ggttaatata cgagatggtt
      241 gataaaataa tatttacggt tactcctatt ttttcaattc ctcctcgcgg agcggctgct
      301 gttgaaacgt ggatgtatca ggttgctcag cgtaccaatt ttccaaatcg tattgtttgt
      361 atcaaaaatc caggatactc aaattacacc tttgttaatg ataattgtag tattcatagg
      421 gttggattca gtcgcatata taagcggtta tttcaaaaat ggacaagact tgatcctctt
      481 ccatattcac agagaatatt gaatattgca catgattttc ctataacgaa agaaagtgtt
      541 attgtagttc ataatagcat gaagctatac actcaaattc gtaagcgagc tcctcaggca
      601 agagtggtta tacatatgca taatgcattt gaaccaaaat tgcttgagca aaatgttaaa
      661 atgatagtgc ctagtttata cttgaaaaag tattaccaat cttatttagc aaatgcagat
      721 attgagattg taccaaatgg aattgattta gaaacctatc aatctaattt tcagccgatc
      781 actagaagcg aacttaatat ctcccctgaa gagaaaatta ttttttatgc tgggcgtatc
      841 gtccctgaca aaggcatatt gctgttgatg caatcattcg aaaaattggc cgctgctcac
      901 aaaaatttaa aattagttgt gattggtgat tatacagaaa tgagtaaaag tgacaaaggt
      961 gcatatcaac gcaatgtcag ggaaattgca aaacgtttga aggatcgttg cattatgtta
     1021 ggaagcatac cgcctgaaaa gatgcattgt tattacccac tggcagattt agtcgtcatt
     1081 ccttcccagt tccaggaacc attttgcatg gtggcaattg aagctatggg tgctggtaag
     1141 cctgttttag tgagtacgcg tggggggatg acggaatttg tcaaagaaaa tactacggga
     1201 ttccatttaa aagaacctat gactgcagac tccataagca gcgatatttt aaaaacgtta
     1261 gcgaatcctg aattaacagc agttgcaaag caaggtcagg actttgtgtt tgatcactac
     1321 agttgggatg gcgtaactca aagattcgaa gaggttattc ataattggtt tgaataaata
     1381 aaaaggccgc attatgcagc cttttttatt ttttacttgt ttttcatcgc taataataag
     1441 ccggcgtaaa cgcctaataa atttggttca atttgttcta cgtt
//
LOCUS       DAZFAB010000288         1475 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZFAB010000288 DAZFAB010000000
VERSION     DAZFAB010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1475
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.291"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            125..1450
                     /gene="nleA"
                     /locus_tag="ACHJWA_005036"
     CDS             125..1450
                     /gene="nleA"
                     /locus_tag="ACHJWA_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector NleA"
                     /protein_id="HFO4311437.1"
                     /translation="MNIQPTIQSGITSQNNQHHQTEQIPSTQIPQSELPLGCQAGFVV
                     NIPDDIQQHAPECGETTALLSLIKDKGLLSGLDEYIAPHLEEGSIGKKTLDMFGLFNV
                     TQMALEIPSSVSGISGKYGVQLNIVKPDIHPTSGNYFLQIFPLHDEIGFNFKDLPGPL
                     KNALSNSNISTTAVSTIASTGTSATTSTVTTEPKDPIPWFGLTAQVVRNHGVELPIVK
                     TENGWKLVGETPLTPDGPKANYTEEWVIRPGEADFKYGASPLQATLGLEFGAHFKWDL
                     DNPNTKYAVLTNAAANALGALGGFAVSRFASTDPMLSPHIGAMVGQAAGHAIQYNTPG
                     LKPDTILWWAGATLGAADLNKAEFEVARFTDYPRIWWHAREGAIFPNKADIEHATGAD
                     IRAMEEGIPVGQRHPNPEDVVIDIESNGLPHHNPSNHVDIFDIIQETRV"
BASE COUNT          484 a          298 c          297 g          396 t
ORIGIN      
        1 taagtcacat caatcaagca ttcaaacagt tatatattaa agctgtccac atcggatatg
       61 tgacactaat agtatcaatg gattgatatt attaatggat ataaacatgt aataaggatt
      121 tattatgaac attcaaccga ccatacaatc tggaatcacc tcacaaaaca atcaacatca
      181 tcaaacagaa caaataccct ctacacaaat accgcaatcc gaattacctc taggatgcca
      241 agctggattt gttgttaata ttccagatga tatacagcaa catgcaccgg aatgcggtga
      301 aacaacagct ctactgagct tgataaaaga taaaggtctg ctctcagggc tagacgaata
      361 tatagctcct caccttgaag aaggatccat aggaaaaaaa acattggata tgtttggttt
      421 attcaatgtt acccaaatgg cattagagat acctagttcc gtttcaggca tctctggtaa
      481 atatggtgtc cagctaaaca ttgtaaaacc agatattcat cctacatcag gtaattattt
      541 tttacagata ttccctctgc atgatgaaat aggttttaat tttaaagacc ttcctggccc
      601 gttaaaaaat gcattaagca acagtaatat atcaaccact gcagtgtcga ctattgcatc
      661 gactggaaca tcagccacta cttcgacggt aaccaccgag ccaaaagacc caataccatg
      721 gtttggatta acagctcaag tggttcgtaa tcatggtgta gaacttccta tagtcaaaac
      781 tgaaaatgga tggaagcttg ttggagaaac accacttact cctgatgggc cgaaagcaaa
      841 ttacacggag gagtgggtta tcagaccggg agaagcagat tttaaatatg gtgcatctcc
      901 attacaggca actctagggc tggagtttgg cgcacatttc aagtgggatt tagataaccc
      961 taatactaaa tatgccgttc ttaccaatgc tgccgcaaat gcgcttggtg ctttaggggg
     1021 atttgcagta tccagatttg ctagtacaga tccaatgtta agtcctcata tcggtgcaat
     1081 ggttgggcaa gcagcagggc atgccataca gtataatacc cctggattaa agccagacac
     1141 tattttatgg tgggctggtg cgacactggg ggctgccgat ttaaacaagg ccgagtttga
     1201 agtagctaga ttcactgact atcctcgtat atggtggcac gcaagagaag gagctatttt
     1261 ccccaataaa gcagatattg aacatgccac aggtgctgat atacgcgcaa tggaagaagg
     1321 tatccctgtt ggacagcggc atccaaatcc agaggatgtg gtaatcgata tcgaaagcaa
     1381 tggcttacca catcataatc catcaaatca tgttgatatc tttgatataa tccaagaaac
     1441 aagagtctaa gctttaactt gtttatttta aaata
//
LOCUS       DAZFAB010000289         1450 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZFAB010000289 DAZFAB010000000
VERSION     DAZFAB010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.292"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            166..453
                     /locus_tag="ACHJWA_005037"
                     /pseudo
     CDS             166..453
                     /locus_tag="ACHJWA_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            897..>1450
                     /gene="espR1"
                     /locus_tag="ACHJWA_005038"
     CDS             897..>1450
                     /gene="espR1"
                     /locus_tag="ACHJWA_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector leucine-rich repeat protein EspR1"
                     /protein_id="HFO4311438.1"
                     /translation="MKFPSIFNKIKPQSIQQHPEKNQLNWMLELNKWKEERILTGEIH
                     RPECRNEAAKRINCAFLSKQNDIDLSGLNLSTQPPGLQNFTSINLDNNQLTHFDATNY
                     DRLVKLSLNSNTLESINIHQGRNVSITHISMNNNCLRNIDIDRLSSITYFSAAHNKLE
                     FVQLESCEWLQYLNLSHNQLTDIVT"
BASE COUNT          541 a          278 c          213 g          418 t
ORIGIN      
        1 aaaatcagca ttcattggtt aatctggaag cgcaaaataa taatctaaaa aattaatatt
       61 tctgatagtt ataaactgaa atttcttaat cttgattata acaagctagc atcgctggat
      121 gtctcccggc aagagtctct gattgagtta agtgcccatc acaatatgat cactgacctt
      181 atattacaca atcatcccag gatgaaaaca atcactttaa acgacaacca tattgcacat
      241 ttaaacacca aaactactac aaaacttgaa tatttaaact taagcaataa caatttattg
      301 ccaaccaatg acattgatca actaatatca tcaaaacatc tttggcatgt attagttaac
      361 ggcatcaaca atgattcact tgcacaaatg cagtactgga ctgcagtaag aaatataatt
      421 gatgacacta atgaagtgac cattgattta tcttataatc tggcaatcac aaatatcgat
      481 accagtgatg aacatcttgt agaagtaagc gataattcag aaggtaatta tataaaagac
      541 aatgactcaa tgtctattcg ttatagatca aaatattatt ccagagattc cgccttaata
      601 gaagaagaaa caatattttc tgacgcagaa ctaaaagcta ttctgcctat gcgtcgcatg
      661 tacggggtta gtgactataa gtcaaattcc tcttctctat cctcacactc gggactcaag
      721 gacccaacgg gcacacccgt ctgttattat attcataatg agagtaaacc ttccttaggt
      781 tatggtccaa catccaataa ttggttaagc caatccttta caacagagtt ataaaacaca
      841 tcctggttta tcaagggtga taaatcagaa aaacattgta ttactatgga gcagttatga
      901 aattcccttc aatatttaac aaaataaaac cacaatccat acagcaacat ccagaaaaaa
      961 atcaacttaa ctggatgctc gaattaaata aatggaaaga agaacgtata cttacaggtg
     1021 aaatccatcg tccggaatgt cgaaacgaag ccgctaaaag gataaactgt gcttttttgt
     1081 cgaaacagaa tgacattgat ttatcaggac ttaatttatc tactcaacca ccagggctgc
     1141 aaaacttcac ctctatcaat cttgataata accaactcac acattttgat gcaaccaact
     1201 acgatagact cgtaaaactt agtctgaata gtaacactct tgagtcaata aatattcatc
     1261 aaggcagaaa tgtaagcatt acacatatat ctatgaataa taattgtctc agaaatattg
     1321 atatagatag gctttcatca attacttatt ttagtgcggc acataataaa ctagagtttg
     1381 tgcaattaga atcttgcgaa tggctgcaat acctgaatct cagccataat caattaactg
     1441 atattgttac
//
LOCUS       DAZFAB010000290         1438 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZFAB010000290 DAZFAB010000000
VERSION     DAZFAB010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1438
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.293"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(34..525)
                     /locus_tag="ACHJWA_005039"
     CDS             complement(34..525)
                     /locus_tag="ACHJWA_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311439.1"
                     /translation="MKPRNINNSLPLQPLVPEQENKNKKNEEKSVNPDKITMGSGLNY
                     IEQESLGGKYLTHDLSIKIADISEEIIQQAILSAMSIYKFPITDDLMSMAVNELIKLT
                     KIENNVDLNKFTTICTDVLSPLVTKHNKQKSQNDILPFAKIPFLIFIEKMEIDNMNPG
                     HVH"
     gene            955..1380
                     /gene="yqeK"
                     /locus_tag="ACHJWA_005040"
     CDS             955..1380
                     /gene="yqeK"
                     /locus_tag="ACHJWA_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeK"
                     /protein_id="HFO4311440.1"
                     /translation="MDIEFSQIHEMVYMHDIVNSDSKKKPRIPLKKFLNAEKVLTQTT
                     SWALNSRFVNVNSVNKVNVKSKVKNSYISRSVNDEFSLTDDEINSFKETLVLSSIDSL
                     SKMVLNNPLSVLFTSTVRRNNNRAKMNVEFDSWICTRCC"
BASE COUNT          449 a          209 c          241 g          539 t
ORIGIN      
        1 ttagttatcc taattatgac atttcaaaat gccttaatgt acatgacctg gattcatatt
       61 atcaatttcc attttttcaa tgaaaattaa aaaggggatt tttgcgaagg gtagaatgtc
      121 gttttgactt ttttgtttat tatgtttggt gacgaggggg gatagaacgt ctgtgcatat
      181 agtagtgaat ttattcaggt ctacattatt ctctattttg gtcagtttta tgagttcatt
      241 tacagccata ctcattaaat catctgttat cggaaattta tatatgctca tagcagataa
      301 tattgcttgc tgaattatct cttcagaaat atccgctatc tttattgata aatcatgtgt
      361 tagatatttc cctccaagag attcttgttc aatataattt aaaccagacc ccattgtaat
      421 tttatctgga ttaacggatt tctcttcatt tttcttattt ttgttctcct gttcaggaac
      481 taatggttgc agtggtaggc tattattaat atttcgtggc ttcattttac atttctctca
      541 tttgattaga gcaaaatccg aattcgcagc ttctgataat gtcaaatatc accttttctc
      601 atgctttatt ctatcaaaaa tcgctcattt ttactttttt tactataatg ttacttagta
      661 gcatcggcaa tttacataac ttacatgttg ttttttatat ccttaaaaaa cagatagata
      721 gtgtgcgtac tcgaaatgat attggtaaca taaatagctt tattcgtgat aaatatctca
      781 aaagatttcc tttattatat tcgaaattca tttgatcaag gttatcacct tatggagacg
      841 ttacgtagaa tgactatatt tgttatcggc acattagggg tattaatttt agtattgatg
      901 gaaggtgatt attgaacagt aacatcgagt cacggtaaaa atgtgaggta tataatggac
      961 attgagtttt cgcagattca tgaaatggtc tatatgcatg atattgtaaa ttcggactcg
     1021 aaaaagaaac caaggattcc gttaaaaaaa ttccttaatg ctgagaaggt tctcacgcaa
     1081 acaacatcat gggcattaaa ttcaagattt gtgaatgtta attctgtcaa taaagtcaac
     1141 gtaaagagta aagttaaaaa cagttatatc tcacgatctg tcaatgatga gtttagttta
     1201 acagatgatg aaatcaattc ttttaaagaa acgttagtcc tttcctctat tgattcactg
     1261 agtaaaatgg ttcttaataa tcctctttct gtattgttta catcgactgt acgaaggaat
     1321 aataatcgag ctaagatgaa tgtcgagttc gactcatgga tttgcaccag atgttgttga
     1381 tacatgatat tttacattgt aaggagatgt ctaaaatgaa agatgttgat caaaactt
//
LOCUS       DAZFAB010000291         1398 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZFAB010000291 DAZFAB010000000
VERSION     DAZFAB010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.294"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..127
                     /locus_tag="ACHJWA_005041"
     CDS             <1..127
                     /locus_tag="ACHJWA_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311441.1"
                     /translation="VSHFGLTVMLYCFIFIYIVNYFIWYKCSFKKHIDRQLNIRG"
     gene            130..1224
                     /gene="rfbE"
                     /locus_tag="ACHJWA_005042"
                     /gene_synonym="perA"
     CDS             130..1224
                     /gene="rfbE"
                     /locus_tag="ACHJWA_005042"
                     /gene_synonym="perA"
                     /EC_number="2.6.1.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-perosamine synthase RfbE/PerA"
                     /protein_id="HFO4311442.1"
                     /translation="MKYIPVYQPSLTGKEKEYVNECLDSTWISSKGNYIQKFENKFAE
                     QNHVQYATTVSNGTVALHLALLALGISEGDEVIVPTLTYIASVNAIKYTGATPIFVDS
                     DNETWQMSVSDIEQKITNKTKAIMCVHLYGHPCDMEQIVELAKSRNLFVIEDCAEAFG
                     SKYKGKYVGTFGDISTFSFFGNKTITTGEGGMVVTNDKTLYDRCLHFKGQGLAVHRQY
                     WHDVIGYNYRMTNICAAIGLAQLEQADDFISRKREIADIYKKNINSLVQVHKESKDVF
                     HTYWMVSILTRTAEEREELRNHLADKLIETRPVFYPVHTMPMYSEKYQKHPIAEDLGW
                     RGINLPSFPSLSNEQVIYICESINEFYSDK"
     gene            1249..>1398
                     /locus_tag="ACHJWA_005043"
     CDS             1249..>1398
                     /locus_tag="ACHJWA_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HFO4311443.1"
                     /translation="MKIALNSDGFYEWGGGIDFIKYILSILETKPEICIDILLPRNDI
                     HSLIRE"
BASE COUNT          498 a          200 c          265 g          435 t
ORIGIN      
        1 agttagccat tttgggttaa ctgttatgtt gtactgcttc atttttatat atattgtaaa
       61 ttactttata tggtataaat gtagttttaa aaaacatatc gatagacagt taaatataag
      121 aggatgaaaa tgaaatatat accagtttac caaccgtcat tgacaggaaa agaaaaagaa
      181 tatgtaaatg aatgtctgga ctcaacgtgg atttcatcaa aaggaaacta tattcagaag
      241 tttgaaaata aatttgcgga acaaaaccat gtgcaatatg caactactgt aagtaatgga
      301 acggttgctc ttcatttagc tttgttagcg ttaggtatat cggaaggaga tgaagttatt
      361 gttccaacac tgacatatat agcatcagtt aatgctataa aatacacagg agccaccccc
      421 attttcgttg attcagataa tgaaacttgg caaatgtctg ttagtgacat agaacaaaaa
      481 atcactaata aaactaaagc tattatgtgt gtccatttat acggacatcc atgtgatatg
      541 gaacaaattg tagaactggc caaaagtaga aatttgtttg taattgaaga ttgcgctgaa
      601 gcctttggtt ctaaatataa aggtaaatat gtgggaacat ttggagatat ttctactttt
      661 agcttttttg gaaataaaac tattactaca ggtgaaggtg gaatggttgt cacgaatgac
      721 aaaacacttt atgaccgttg tttacatttt aaaggccaag gattagctgt acataggcaa
      781 tattggcatg acgttatagg ctacaattat aggatgacaa atatctgcgc tgctatagga
      841 ttagcccagt tagaacaagc tgatgatttt atatcacgaa aacgtgaaat tgctgatatt
      901 tataaaaaaa atatcaacag tcttgtacaa gtccacaagg aaagtaaaga tgtttttcac
      961 acttattgga tggtctcaat tctaactagg accgcagagg aaagagagga attaaggaat
     1021 caccttgcag ataaactcat cgaaacaagg ccagtttttt accctgtcca cacgatgcca
     1081 atgtactcgg aaaaatatca aaagcaccct atagctgagg atcttggttg gcgtggaatt
     1141 aatttaccta gtttccccag cctatcgaat gagcaagtta tttatatttg tgaatctatt
     1201 aacgaatttt atagtgataa atagcctaaa atattgtaaa ggtcattcat gaaaattgcg
     1261 ttgaattcag atggatttta cgagtggggc ggtggaattg attttattaa atatattctg
     1321 tcaatattag aaacgaaacc agaaatatgt atcgatattc ttttaccgag aaatgatata
     1381 cattctctta taagagaa
//
LOCUS       DAZFAB010000292         1398 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZFAB010000292 DAZFAB010000000
VERSION     DAZFAB010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.295"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>1398
                     /gene="rhsB"
                     /locus_tag="ACHJWA_005044"
     CDS             <1..>1398
                     /gene="rhsB"
                     /locus_tag="ACHJWA_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhs element protein RhsB"
                     /protein_id="HFO4311444.1"
                     /translation="ASAFYYNHHSQLTSATGPDGLEIRREYDEWGRLIQETAPDGDIT
                     RYRYDNPHSDLPCATEDATGSRKTMTWSRYGQLLSFTDCSGYVTRYDHDRFGQMTAVH
                     REEGLSQYRAYDSRGQLIAVKDTQGHETRYEYNAAGDLTTVIAPDGSRNGTQYDAWGK
                     AICTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQETGFDGRTQRYHHD
                     LTGKLIRSEDEGLVTHWHYDEADRLTHRTVKGETAERWQYDERGWLTDISHISEGHRV
                     TVHYGYDEKGRLTGERQTVHHPQTEALLWQHETRHAYNAQGLANRCIPDSLPAVEWLT
                     YGSGWLAGMKLGDTPLVDFTRDRLHRKTLRRFGRYELTTAYTPAGQLQSQHLNSLQYD
                     RDYTWNDNGELIRISSPRQTRSYSYSDSGRLTGVHTTAANLDIRIPYATDPAGNRLPD
                     PELHPDSTLSMWPDN"
BASE COUNT          335 a          439 c          409 g          215 t
ORIGIN      
        1 gggcatcggc gttttactat aaccaccaca gccagttaac gtcagccacc gggcctgacg
       61 ggctggaaat acgccgggaa tatgatgaat ggggccgtct gattcaggaa actgcccctg
      121 acggcgatat cacccgctac cgttatgata atccacacag tgacttaccc tgcgcaacgg
      181 aagatgccac cggcagccgg aaaaccatga cgtggagccg ttacggtcag ttgctgagct
      241 tcaccgactg ttccggttat gtaacccgtt atgaccatga ccgcttcggg cagatgacgg
      301 cggtgcaccg cgaggaaggg ctgagtcagt accgcgcata cgacagccgt ggacagttaa
      361 ttgccgtgaa agacacgcag ggccatgaaa cgcggtatga atacaacgcc gccggtgacc
      421 tgaccaccgt cattgccccg gacggcagca gaaacgggac acagtacgat gcgtggggaa
      481 aagccatctg taccacgcag ggcggtctga cgcgcagtat ggaatacgat gctgccggac
      541 gggtcatccg cctgaccagt gaaaacggca gccacaccac cttccgttac gatgtactcg
      601 accggctgat acaggaaacc ggctttgacg gccgcacaca gcgttatcac cacgacctga
      661 ccggcaaact tatccgcagc gaggatgagg ggctggtcac ccactggcac tatgacgaag
      721 cagaccgcct cacgcaccgc accgtgaagg gtgaaaccgc agagcgctgg cagtatgacg
      781 aacgcggctg gctgacagac atcagccata tcagcgaagg gcaccgggtg acggtgcatt
      841 acgggtatga tgagaaaggc cggctgaccg gtgagcgtca gacggtgcat cacccgcaga
      901 cggaagcact gctctggcag catgagacca gacacgctta caacgcgcag gggctggcga
      961 accgctgtat accggacagc ctgcccgccg tggaatggct gacctatggc agcggctggc
     1021 tggcaggcat gaagctcggc gacacaccgc tggtggattt cacccgcgac cgcctgcacc
     1081 ggaaaacgct gcgcagattc ggccgttatg aactcaccac cgcttatacc cctgccgggc
     1141 agttacagag ccagcacctg aacagcctgc agtatgaccg cgattacacc tggaacgaca
     1201 acggcgaact catccgcatc agcagcccgc gccagacccg gagttacagc tacagcgact
     1261 ccggcaggct gaccggcgtt cacaccaccg cagcgaatct ggatatccgc atcccgtatg
     1321 ccacggaccc ggcaggtaac cgcctgcccg acccggagct gcacccggac agcaccctca
     1381 gcatgtggcc ggataacc
//
LOCUS       DAZFAB010000293         1355 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZFAB010000293 DAZFAB010000000
VERSION     DAZFAB010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.296"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(16..270)
                     /locus_tag="ACHJWA_005045"
     CDS             complement(16..270)
                     /locus_tag="ACHJWA_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFO4311445.1"
                     /translation="MTKVRDIAPYSVRMPDSLKRDLTIRASKNGRSLNSEIVMILQAA
                     IDEEKSPRSIEGFAQQESEKFREALLKTLSSMYGEDKKPT"
     gene            380..490
                     /locus_tag="ACHJWA_005046"
     CDS             380..490
                     /locus_tag="ACHJWA_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFO4311446.1"
                     /translation="MEKEVSRILVRIPQSLKDAITGKAKEECRSFNSEVM"
     gene            793..>1355
                     /locus_tag="ACHJWA_005047"
     CDS             793..>1355
                     /locus_tag="ACHJWA_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HFO4311447.1"
                     /translation="MKSIAKAQNDFTIFKFGDSEIRVINKCGEPWFVAKDVCDALALT
                     NSRKALTALDDDEKGVTLSYTLGGEQNLSIVSESGMYTLVLRCRDAVNKGSVPHKFRK
                     WVTAEVLPSIRKHGEYVKGKKTTVEERTPLRDAVNMLVGKKGLRNDDAYNMVHQRFGI
                     DSIDELSIEQIPLAVEYIHRVVLEGEF"
BASE COUNT          407 a          251 c          322 g          375 t
ORIGIN      
        1 ggattatgcc caccatcagg tgggtttttt atcttcgccg tacatgctgc tgagagtctt
       61 aagaagagcc tccctaaatt tttcagattc ttgttgagca aaaccttcta ttgatcttgg
      121 tgatttttct tcatcaatag cagcttgcaa gatcataact atttctgaat ttaatgagcg
      181 tccgtttttc gatgctctta tggtcaggtc gcgcttgagg ctatctggca ttcttacgct
      241 atatggggct atgtctctaa ccttcgtcat acttacaccg tgataatcac attgatatca
      301 cagtgtattc aaaaaaactt tgacttgata tagtcacagt gatacgtttg tgattcataa
      361 taactcacgc ggtggcgata tggaaaaaga agttagcaga attttggtga gaatacctca
      421 gtcgctaaaa gatgcgatta caggaaaggc caaagaagaa tgccggtcgt ttaactcgga
      481 agtgatgtga ttgctggaca gcctgaagag agaggggata acggtatgag taaagaatgt
      541 tgttttttgc ggcattagcg aatcagacgc tgatcaaaca tacatttact ctaaagaaac
      601 aggtcggatg ctgtgtagtg actgcgtgtt tgacatcata agatacaagt atcttggatg
      661 ttctgccagc attagcaata taggtgaagt gtacgaaggg aaagatataa ctgatagagc
      721 agaaagttga agccccaact gctgtaacag tcagggcttc gttatcaaca aatcggctta
      781 ggaaatattg acatgaaaag tatagcaaag gcacaaaacg atttcactat cttcaaattc
      841 ggcgacagtg aaatccgcgt catcaacaag tgcggtgagc cgtggtttgt agctaaagat
      901 gtttgtgatg ctttagcttt gactaactca cgcaaggcgc ttactgcact tgatgacgat
      961 gaaaagggag taactttaag ttacaccctt ggtggtgagc agaatctaag cattgtgagc
     1021 gaatcaggta tgtatacatt ggttctgcgc tgccgcgatg cagtcaataa aggttcagtc
     1081 ccgcacaaat tccgcaagtg ggtaacagca gaagttctgc cttcaattcg caaacatggc
     1141 gagtatgtaa aaggaaagaa aaccactgtt gaggaaagaa caccgctacg cgatgcagta
     1201 aacatgctgg taggaaagaa aggacttcgc aatgacgatg catacaatat ggttcatcag
     1261 cgttttggta ttgacagcat tgatgagctt tcaattgaac aaatcccgct tgccgtagag
     1321 tacatccaca gggtagtgct tgaaggtgaa ttcat
//
LOCUS       DAZFAB010000294         1342 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZFAB010000294 DAZFAB010000000
VERSION     DAZFAB010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.297"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            81..728
                     /gene="nleG"
                     /locus_tag="ACHJWA_005048"
     CDS             81..728
                     /gene="nleG"
                     /locus_tag="ACHJWA_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase NleG"
                     /protein_id="HFO4311448.1"
                     /translation="MPVILNFSSERVLSESELEALRHVGRVSQSEQLVVRGRTMRLHH
                     ISFMDSFSVEPVSGGLLDRLSARGHRLLAENLEIQLNRGHTFLQAFRLYMEQSRATPC
                     TRQNVSSAIQNKINSHAFTVSHQDFSCHEQHLNCPITLCIPETGVFVRNAKNSEICSL
                     YDHNALTELIRRNAPHPLSREPFVPEMIVSKDECHFNLIEQYFCILATQNICTRI"
     gene            complement(865..1011)
                     /locus_tag="ACHJWA_005049"
     CDS             complement(865..1011)
                     /locus_tag="ACHJWA_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311449.1"
                     /translation="MIFIASSSREQTTASDGKMGTTGYSGNLAEEAYGTDCNVKYKTL
                     KIPF"
BASE COUNT          410 a          280 c          253 g          399 t
ORIGIN      
        1 tttgaatcct atcacatatt tagtatcttg actcctgtca ccaatatggt tcaataacat
       61 agactaaaga gggagaaaat atgcctgtca tattaaactt ttcgagtgaa agagtattgt
      121 ctgaaagtga actggaggct ttacgtcatg tcggaagagt tagtcagagt gaacagcttg
      181 ttgtgagagg ccgcactatg agacttcatc atatttcatt tatggacagt tttagcgtgg
      241 aaccggtttc cggcggactg ttggatcgcc ttagtgcaag agggcatcgc cttctcgcag
      301 aaaatctgga gatacagctt aacagaggcc atactttttt gcaggcattc cgtttatata
      361 tggaacagag ccgtgcaacc ccatgtacgc gccaaaacgt cagtagtgca atacaaaaca
      421 aaattaactc tcatgcattt actgttagtc atcaagactt ttcctgtcat gaacaacacc
      481 tgaattgccc gataacatta tgcattcctg aaactggtgt ttttgtcaga aatgcaaaaa
      541 actcagaaat atgttcatta tatgaccaca atgcactcac tgaacttatc cgacgcaacg
      601 ctccacaccc tctcagccgt gaaccctttg ttccggaaat gattgtcagc aaagatgaat
      661 gccattttaa tctaatagaa caatattttt gcatattagc cacacaaaat atatgcacta
      721 gaatttgact gtatggttcg atttaaatgt tgcaaaatta attttactag ttattgccat
      781 ctatttcata ctccctaatt cttgatacaa tctgtctgtc aaaatagtag attagatcga
      841 acttatatta cagctattta gtttctaaaa tggaattttt aacgttttat acttcacatt
      901 acaatctgta ccataagcct cctcagcgag gttgcctgaa taccctgtcg tgcccatttt
      961 tccatcggac gcagttgtct gctctctcga actactggca ataaaaatca accagacagc
     1021 ttgatgtccg ttacatgcag gactaatcac gattggttac cgtttacaaa acttgcgtaa
     1081 cattggagga gaacgaataa tattctctag ctggtacgcg ttccatagcg aaacgggcaa
     1141 ggtccaacag caagagctga aactcagggg gatttatcag tcgatttatt cgccatggat
     1201 gaatcacgct gtacggttat ggtagctctt agcgacaagg cataatcatc agtacaccaa
     1261 tgtaacaacc gttgaaaaag actcattatt ttatgaacca gccatcccat ttccagagtg
     1321 gtaaatatga tttgttaaag tg
//
LOCUS       DAZFAB010000295         1325 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZFAB010000295 DAZFAB010000000
VERSION     DAZFAB010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.299"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..302)
                     /gene="cesF"
                     /locus_tag="ACHJWA_005050"
     CDS             complement(<1..302)
                     /gene="cesF"
                     /locus_tag="ACHJWA_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesF"
                     /protein_id="HFO4311450.1"
                     /translation="MNEKFRTDLAHTFGIALEEQTDVLSFHDNDGHEWILECASQSEI
                     LFFYCYLLNSESIQINSILEMNSNRELLGMFFLSLKDDNILLNIAFPADKIDITEFA"
     gene            568..1179
                     /gene="map"
                     /locus_tag="ACHJWA_005051"
     CDS             568..1179
                     /gene="map"
                     /locus_tag="ACHJWA_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector Map (Rho
                     guanine exchange factor)"
                     /protein_id="HFO4311451.1"
                     /translation="MFSPMTMAGRSLVQATAQTLKPAVTRAAMQAGTGATGMRFMPVQ
                     SNFVINHGKLTNQLLQAVAKQTRNGDTQQWFQQEQTTYISRTVNRTLDDYCRSNNSVI
                     SKETKGHIFRAVENALQQPLDMNGAQSSIGHFLQSNKYFNQKVDEQCGKRVDPITRFN
                     TQTKMIEQVSQEIFERNFSGFKVSEIKAITQNAILEHVQDTRL"
BASE COUNT          442 a          225 c          267 g          391 t
ORIGIN      
        1 gcaaactctg taatatcaat tttatctgca ggaaatgcaa tatttaaaag aatgttgtca
       61 tcttttaatg acaggaaaaa cataccgagc agttctcgat tgctattcat ttcaagtatt
      121 gaattaatct gtattgattc gctattcaat aaataacaat aaaaaaacag aatttcagat
      181 tgagatgcac actccagtat ccattcatgg ccatcgttat cgtggaaaga gagcacgtca
      241 gtttgctctt caagagctat cccaaacgta tgtgcaaggt ctgtgcgaaa tttctcattc
      301 atattgtagt cctgccattc ttttcatgtt gatatactca aacgtaatta atccgagagt
      361 tctgatacaa ataataaggc gtagatataa gattacggga atagtgttgc atattcatga
      421 tagcatatga taaatgtagt gcaactacct gtagataagg ccggaggata attcaaatat
      481 aaatacaaga taattattct ttaaagttat tttttaatgg gaagcaattt ttttactgtc
      541 gaaatataat ttataaaggt ttatggtatg tttagtccaa tgacaatggc aggcagatcg
      601 ttagttcagg cgactgcaca gacgcttaaa cctgcggtta ccagagctgc aatgcaagcg
      661 ggtacgggag ccacgggtat gaggttcatg cccgtgcagt cgaactttgt gattaatcat
      721 ggcaaactga ctaaccaact cctgcaggct gtagccaaac aaactcgtaa tggcgatacc
      781 caacaatggt tccagcagga gcagacgact tatatatcca gaacagtaaa tagaactctg
      841 gatgactatt gcaggagtaa taattcggtc attagtaagg agactaaagg tcacattttt
      901 agagctgttg aaaatgcgtt acagcaacct cttgatatga acggggcaca atcatcaatt
      961 ggtcacttct tgcagtccaa taaatacttt aatcagaaag ttgatgagca gtgcggcaaa
     1021 agagtggatc caataacacg ttttaacacc cagacgaaga tgatagaaca agtctcacaa
     1081 gaaatatttg aacgcaactt tagcggcttc aaagtcagtg agataaaagc gattactcag
     1141 aatgcgattt tagagcatgt acaggatacc cgattgtagg gataaccttg tcaggtataa
     1201 attaagcttc ctggtgtata gcatggcctt tgttgatgta agaaaaaacg cgataaagaa
     1261 acttaataaa ctttattatt aattaagatt tatagtttgc ttaattggtt ttctttggct
     1321 gtgtt
//
LOCUS       DAZFAB010000296         1308 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZFAB010000296 DAZFAB010000000
VERSION     DAZFAB010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.300"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..1300)
                     /locus_tag="ACHJWA_005052"
     CDS             complement(<1..1300)
                     /locus_tag="ACHJWA_005052"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:1900727 - osmoregulated periplasmic glucan
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO4311452.1"
                     /translation="MIKKISINFLFLMLMIDVVFATLFNIPVWMHLFNIINNLDGVKL
                     GFIISLPVFLISALNFVFTPFSFRYIFKPFFCILFICSSIVTYATMKYGVQFDKTMMQ
                     NIFETNAGEMTSYFNMSVVLWFLFTGILPCGLLLLVNIRYPETWIKGITYRLISMFAS
                     LLIIFAIAFFFYKDYASVGRNNSSLNKEIIPTNYIYSGFKYVRDFFVSPGEFRQTGTD
                     ASRTINEKQKPVIMFLVVGETARSQNYALNGYSRGTNDFTKKYNELISFHNVQSCGTS
                     TAISVPCMFSDMKRKEFNSRKAVNSENVLDILYRTGVNLLWIENDGGCKGVCKRIPTI
                     NIEPSNSDNTLCKKNSCYDEVMLKNIDEYINNNSEDKLIVFHLMGSHGPTYYLRYPES
                     HKYFKPTCDRSDIENCTHEQLINTYDNTIRYTDYIISKLID"
BASE COUNT          466 a          233 c          193 g          416 t
ORIGIN      
        1 tgtcaattaa ttttgatatt atataatcag tatagcgaat tgtattatca taggtattaa
       61 tcaattgctc atgcgtacaa ttttcgatat cactacgatc acatgttggt ttaaaatatt
      121 tatgtgactc aggataacgg agatagtagg taggaccatg actacccatc aaatgaaata
      181 cgataagttt atcctctgag ttattattta tatactcatc aatatttttt agcataacct
      241 catcataaca tgagtttttc ttgcataatg tattatctga atttgatggc tcaatattaa
      301 ttgtagggat tcgcttacat acacctttac atcccccgtc attttcaatc cagagtaaat
      361 ttacccccgt tctgtacaga atatccagta cattttccga atttaccgct ttgcgggaat
      421 tgaactcttt tcgcttcata tctgaaaaca tgcatggaac agatatggct gtggatgtgc
      481 cacacgactg cacattatga aaggatataa gttcattata tttctttgta aaatcattgg
      541 taccacgaga atatccattc agagcatagt tttgacttct tgctgtctcc ccgacaacta
      601 aaaacataat taccggcttt tgtttttcat ttattgtgcg ggatgcatcc gttccagtct
      661 gacgaaactc gccaggagaa acaaaaaaat cccttacata tttaaaccca ctgtagatat
      721 aattagtagg tattatttct ttatttaatg aagagttgtt tctgccaact gatgcataat
      781 ccttataaaa gaaaaacgca atcgcaaata taatcaagag agatgcaaac attgatatta
      841 aacgatatgt aatacccttt atccatgtct caggataacg gatatttact aacaacaata
      901 gtccacatgg aagtataccc gtaaataaaa accacagtac aacagacata ttgaaatatg
      961 atgtcatttc acctgcattt gtttcaaaaa tattctgcat cattgtttta tcaaactgca
     1021 caccatattt catggtggca tatgtaacaa tggaactaca gataaacaga atacaaaaaa
     1081 aaggtttaaa tatatacctg aatgagaacg gtgtaaaaac aaaatttaat gcagatatca
     1141 gaaagactgg aagagatata ataaatccaa gtttcacacc atcaaggtta ttaataatat
     1201 taaacagatg catccagaca gggatattga acaatgtggc aaaaacaaca tctatcatta
     1261 acatcaagaa aagaaaatta atactgattt ttttaatcat cttacccc
//
LOCUS       DAZFAB010000297         1305 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZFAB010000297 DAZFAB010000000
VERSION     DAZFAB010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.301"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..1073)
                     /locus_tag="ACHJWA_005053"
     CDS             complement(<1..1073)
                     /locus_tag="ACHJWA_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HFO4311453.1"
                     /translation="MANKTEKPTQKKLQDASKKGQILKSRDLTVSVIMLVGTLYLGYV
                     FDVHHIMSILEYILDHNAKPDIWDYFKAMGIGWLKTIIPFLLVCMFTTILVSWFQSKM
                     QLATEAVKLKFDSLNPVNGLKRIFGLKTVKEFVKAILYIIFFALEIKVFWSNHKSLLF
                     KTLDGDIISLLSDWGEMLFLLILYCLGSMIIVLIFDFIAEYFLFMKDMKMDKQEVKRE
                     YKEQEGNPEIKSKRRERHQEILSEQLKSDVSNSRLMIANPTHIAIGIYFKPHLSPIPL
                     ISVRETNEVALAVRKYAKEIGIPIITDKKLARKIYATHRRYDYVSFENIDEILRLLLW
                     LEDVENAGQPVPDEQLSSEDKYI"
     gene            complement(1082..>1305)
                     /locus_tag="ACHJWA_005054"
     CDS             complement(1082..>1305)
                     /locus_tag="ACHJWA_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFO4311454.1"
                     /translation="VYASPVIAVMLGGEAVLGLLARYASQLNAFAISLTVKSALAFLI
                     LIIYFGPILAERVMPLSFFPEQLQLYIEK"
BASE COUNT          428 a          249 c          208 g          420 t
ORIGIN      
        1 tcaatatatt tatcttctga agagagctgt tcatctggaa caggttgtcc agcattctcc
       61 acatcttcaa gccacagcag aagacgtaat atttcatcta tattttcgaa gctaacataa
      121 tcgtagcgac gatgggtagc ataaattttt cgtgctaatt ttttatctgt aataattggt
      181 atcccgattt cctttgcata tttccttaca gctaatgcta cctcattagt ttctcttaca
      241 gaaatcaatg gaataggtga cagatgtggc ttaaagtata tccctattgc aatgtgagta
      301 gggttggcaa tcatcaaacg gctattactg acatcagatt tcaattgctc agaaagaatt
      361 tcctgatggc gctctctgcg tttagactta atttcaggat ttccttcttg ttccttgtat
      421 tctcttttaa cttcttgttt atccattttc atatctttca taaataaaaa atattcagcg
      481 ataaaatcaa aaattaagac aattatcata ctgccgagac aatacagtat gagaaggaat
      541 agcatctctc cccaatctga taataaagat atgatatctc catcaagagt tttaaaaagc
      601 agtgatttat gattactcca aaatactttg atctccaatg caaaaaaaat aatataaaga
      661 attgctttaa caaattcttt tacggttttt aagccaaata tacgctttaa accatttact
      721 ggattcaatg aatcaaactt taattttaca gcttcagttg ctaattgcat tttactttga
      781 aaccaggaga caagtattgt tgtgaacatg caaaccagca aaaaaggaat gatcgttttc
      841 aaccaaccaa tccccatagc tttaaaatag tcccaaatat ccggtttagc gttatgatca
      901 aggatatatt caagaatcga cataatgtga tgcacatcaa aaacatatcc gagatataaa
      961 gtacccacaa gcattattac agaaacggtt aagtctctac tttttagaat ttgccctttt
     1021 ttagaagcat cctgcagttt cttttgtgtt ggcttttcag ttttatttgc catgaaacac
     1081 tctatttctc aatgtatagt tgcaattgtt ctgggaaaaa ggataacggc atcacacgtt
     1141 cagccagtat tgggccaaaa taaataatta atattaaaaa tgccagcgca cttttgacag
     1201 tcagtgaaat ggcaaaagca tttagttgtg aagcatagcg tgccagcaat cccaggacag
     1261 cctcaccacc cagcataacg gcaattacgg ggcttgcgta aacga
//
LOCUS       DAZFAB010000298         1301 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZFAB010000298 DAZFAB010000000
VERSION     DAZFAB010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.302"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..1182
                     /locus_tag="ACHJWA_005055"
     CDS             <1..1182
                     /locus_tag="ACHJWA_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO4311455.1"
                     /translation="RRMAKRVWRRERDLTGWMSLSRKPEVTWYGWDGDRLTTVQTDTT
                     RIQTVYEPGSFTPLIRVETENGEREKAQRRSLAETLQQEGSENGHGVVFPAELVRLLD
                     RLEEEIRADRVSSESRAWLAQCGLTVEQLARQVEPEYTPARKVHFYHCDHRGLPLALI
                     SEDGNTAWRGEYDEWGNQLNEENPYYLHQPYRLPGQQHDEESGLYYNRNRYYDPLQGR
                     YITQDPIGLAGGWNLYNYPLNPIIRMDPLGLYNLYQLLYDVWHDDSYGTSSIDITGSG
                     DLISLGGHAGLGVAFAKKKGEMLSDICIYATACGHAGIGGGINAAITYSETKSLPTSG
                     VSNSVGVTVGGGVGGHFAYTYVVDVDNPESSTESVGIGAGVDASVMTLACRTWQECWV
                     N"
     gene            1194..>1301
                     /locus_tag="ACHJWA_005056"
     CDS             1194..>1301
                     /locus_tag="ACHJWA_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-e4"
                     /protein_id="HFO4311456.1"
                     /translation="MKKYIFLVLFLLYGCSPVDIEPARQEVNIFRGLYQT"
BASE COUNT          335 a          260 c          405 g          301 t
ORIGIN      
        1 cggcgaatgg caaaacgggt ctggcggcgg gagcgtgacc tgacggggtg gatgtcgctg
       61 tcgcgtaaac cggaggtgac gtggtatggc tgggacggag acaggctgac gacggtgcag
      121 actgacacca cacgtatcca gacggtatac gagccgggaa gcttcacgcc gctcatccgg
      181 gtcgagacag agaacggcga gcgggaaaaa gcgcagcggc gcagcctggc agagacgctc
      241 cagcaggaag ggagtgagaa cggccacggc gtggtgttcc cggctgaact ggtgcggctg
      301 ctggacaggc tggaggaaga aatccgggca gaccgcgtga gcagtgaaag ccgggcgtgg
      361 cttgcgcagt gcgggctgac ggtggagcaa ctggccagac aggtggagcc ggaatacaca
      421 ccggcgcgaa aagttcattt ttaccactgc gaccaccggg gcctgccgct ggcgctcatc
      481 agcgaagacg gcaatacggc gtggcgcggg gagtatgatg aatggggcaa ccagcttaat
      541 gaagagaacc cgtattacct gcaccagcca taccgtctgc cggggcagca gcatgatgag
      601 gaatcagggc tgtactataa ccggaaccgg tactatgacc cgctacaggg gaggtatatt
      661 acacaagacc ccattgggct ggcgggggga tggaatctgt ataattaccc actgaatccg
      721 ataataagga tggatccttt gggtttgtat aatttatatc aattattata tgatgtttgg
      781 catgatgatt catatggaac atcatcaatt gatattactg gcagtggaga tctaatatca
      841 ttaggtggtc atgcaggact tggcgttgcg tttgctaaaa agaaaggtga aatgttatct
      901 gatatttgta tttatgctac agcatgcgga catgcaggaa ttggtggtgg gataaatgcg
      961 gctatcacat attctgagac caagtcttta cctacatcgg gagtcagcaa ttcagtaggt
     1021 gtaacggttg gcggcggagt tggggggcat tttgcgtata cttatgtagt ggatgttgat
     1081 aatccagaat catcgacaga atctgttggt atcggtgcag gtgttgacgc ttcagttatg
     1141 actctggctt gtagaacgtg gcaagaatgc tgggtcaatt aacaggagga aatatgaaaa
     1201 aatatatatt tttagtttta tttttattgt atggttgttc tccagtggac atagagccag
     1261 ctagacaaga agttaatatc tttcgtggtt tatatcagac a
//
LOCUS       DAZFAB010000299         1274 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZFAB010000299 DAZFAB010000000
VERSION     DAZFAB010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.303"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..420
                     /gene="espJ"
                     /locus_tag="ACHJWA_005057"
     CDS             <1..420
                     /gene="espJ"
                     /locus_tag="ACHJWA_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     ADP-ribosyltransferase EspJ"
                     /protein_id="HFO4311457.1"
                     /translation="RIQNNQFTDLKNKKIQGHQNTVASVMDWYNPQKNALGITMGTPR
                     KSADIAKEEHRNALNFMIMEKNTFHEKILNSNDNLQKSYSKTEDSSWVAASVGSLLDK
                     GAKVYPDTSCSLRLGEPFIFTLPESVRVDVDIYPLKK"
     gene            745..>1274
                     /gene="tccP"
                     /locus_tag="ACHJWA_005058"
                     /gene_synonym="espF(U)"
     CDS             745..>1274
                     /gene="tccP"
                     /locus_tag="ACHJWA_005058"
                     /gene_synonym="espF(U)"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tir-cytoskeleton coupling protein TccP"
                     /protein_id="HFO4311458.1"
                     /translation="MINNVSSLFPTVNRNITAVYKKSSFSVSPQKITLNPVKISSPFS
                     PSSSSISATTLFRAPNAHSASFHRQSTAESSLHQQLPNVRQRLIQHLAEHGIKPARSM
                     AEHIPPAPNWPAPPPPVQNEQSRPLPDVAQRLVQHLAEHGIQPARNMAEHIPPAPNWP
                     APPLPVQNEQSRPLPDV"
BASE COUNT          432 a          287 c          225 g          330 t
ORIGIN      
        1 agaatccaaa acaatcagtt tactgatttg aaaaacaaaa aaattcaagg ccatcaaaac
       61 acagttgcca gtgtcatgga ttggtataat ccccaaaaaa atgctttggg tataactatg
      121 ggtacaccaa gaaaatcggc cgatatagca aaagaagagc acagaaatgc actaaatttc
      181 atgataatgg aaaaaaacac atttcatgaa aaaatcttaa acagcaatga caatttgcaa
      241 aaatcataca gcaaaacaga agacagtagt tgggtcgcag cttctgtcgg ctctcttcta
      301 gataaaggag caaaagtata tcccgataca tcatgctctc tgagactggg agaaccattc
      361 atctttacgc tgccagaatc agttagagtc gatgttgaca tatatcctct caaaaaataa
      421 tcttaaaggt attgacatat aagaaataat taagtgtatt cacattagac tagtcgctta
      481 agagtagatt tttggcattt tgggaagatc agcgggacat aagagacata caacacagga
      541 ggaatggctg ttttttaata gtgctttgaa ttccgcatga aacctttctt agcagtcgaa
      601 aagcaaaaca tacgttttct ttttaacaat aattgatgag tattgcatcg agtgtcattt
      661 atacatcgcc gcatttattt gccactataa aaaggctgat tacatatcta tcggagaact
      721 aaaattactt ataaggtaat ctgcatgatt aacaatgttt cttcactttt tccaaccgtc
      781 aaccgcaata ttacagctgt atataaaaaa agcagcttct ctgtatcacc acagaaaatc
      841 acattaaatc ctgtaaaaat cagctcacct ttttcaccaa gcagtagctc catcagcgca
      901 acaactctct ttcgagcccc aaacgcccat tcggcatcat ttcatcgaca gtctactgct
      961 gaaagttcgt tacatcaaca acttcctaat gtgaggcagc gcctgataca acatcttgca
     1021 gagcatggca ttaaacctgc ccggagtatg gctgaacata ttcctccggc acctaactgg
     1081 cctgcgccac caccgccagt acaaaatgaa caatcaagac ctctgcctga tgtggctcag
     1141 cgtctggtgc agcatcttgc agagcatggc attcaaccag cccggaatat ggctgaacat
     1201 attcctccgg cacctaactg gcctgcgcca ccactgccag tacaaaatga acaatcaaga
     1261 cctctgcctg atgt
//
LOCUS       DAZFAB010000300         1265 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZFAB010000300 DAZFAB010000000
VERSION     DAZFAB010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.304"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            639..929
                     /locus_tag="ACHJWA_005059"
     CDS             639..929
                     /locus_tag="ACHJWA_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO4311459.1"
                     /translation="MNLSASMNDAGDGFELLMSGNMQDFNICCRRDNHYPEHKLIPLS
                     ELFDVNYGLNLELNKLEKDSSGINFVSRTSKNNGVSARVKLKDGISPCQQLC"
     gene            953..1204
                     /locus_tag="ACHJWA_005060"
     CDS             953..1204
                     /locus_tag="ACHJWA_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO4311460.1"
                     /translation="MESFVQDSPFYSGRDLYWLRPKVELTLEEKLYYCSCIRRNRHKY
                     SYGRQANRTLKNLLVPSLDSVPAWVYGVTGKRISELSER"
BASE COUNT          357 a          228 c          294 g          386 t
ORIGIN      
        1 cagttaacat taacaggatt ttgtaataag ccacatttat tctttcggga attaaaaaac
       61 ataacgaaat tttgcctcat tctgcatttc attgaaatag tatgtttctc atcgaaagca
      121 agtttgtcaa tataaatttg taacagaaat gactttccgg ggataaacaa aactgttcat
      181 ggcaatggct ttctctgtcg ccaacatgga tacattgttc cggataaagc actgatgtca
      241 ttgactgacg cagactatca ggccatctga tgctttcttt tccttcaccg gtattgatga
      301 tatgccttat gaagggggac ttattcctgt aacccaatca caggcactgt gattactggt
      361 ttatgattga tgctgatttg agcagatatg cacagcagtt gtggattcag gggatatttt
      421 ctgccagata ttcataccag attgatgacc tctttgctca gatgctcttc gttctgagcc
      481 agccaggatt ttgtggccac atacattgtt cgccgatggg gaatcgagac aggcgattgg
      541 ctgtgatgcg attttgccgc taactgagga tgagtccgcc cctcccgggg aattcctgta
      601 agttaagccg tacctgttga ttagcactaa actgggtaat gaatttatct gccagtatga
      661 atgatgccgg ggacggtttt gaactcctga tgtcgggtaa tatgcaggat tttaatattt
      721 gttgtcgtcg ggataatcat tatcctgaac ataaactgat acctttatca gagttatttg
      781 acgttaatta tggactgaat cttgagctga ataaactgga gaaagattct tcaggaatta
      841 attttgtttc aagaacgtcc aaaaataatg gcgtctccgc cagggttaaa ttaaaagatg
      901 gtattagtcc atgccagcag ctgtgttaac tgtagcagca ggaggttctg ttatggaatc
      961 gtttgtacag gattcaccat tttatagtgg aagagatctt tattggttac gtcctaaagt
     1021 tgaactgact ctggaagaga aattgtacta ctgctcttgt ataagaagaa acagacacaa
     1081 atacagctac gggcgtcagg caaaccgaac gctgaagaat ttactggtac cgtcattaga
     1141 ttctgttccg gcatgggtat atggtgtgac agggaaaaga atatctgaat tgtctgaacg
     1201 ttgagactaa gagggccgga ggccaggtga atggccaccg ggatatttat atcagagtat
     1261 attgg
//
LOCUS       DAZFAB010000301         1263 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZFAB010000301 DAZFAB010000000
VERSION     DAZFAB010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.305"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..348)
                     /locus_tag="ACHJWA_005061"
     CDS             complement(<1..348)
                     /locus_tag="ACHJWA_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311461.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPTGNNLEALAPGIEKLKQ
                     TSIEMVTLLNTLQPSGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNAQIIQRSD
                     NTCELTINEPLKNQ"
     gene            complement(370..909)
                     /gene="yhaB"
                     /locus_tag="ACHJWA_005062"
     CDS             complement(370..909)
                     /gene="yhaB"
                     /locus_tag="ACHJWA_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaB"
                     /protein_id="HFO4311462.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEKCTCSAVNKKELECIKETISKYCAKFTRKEAILTPPAYFNKTSIT
                     SDCWQILFFSPDHFNNDFY"
     gene            complement(1165..>1263)
                     /locus_tag="ACHJWA_005063"
     CDS             complement(1165..>1263)
                     /locus_tag="ACHJWA_005063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HFO4311463.1"
                     /translation="ISNTGIDHLEQLINVNFFSSDRTSFCECNRFP"
BASE COUNT          385 a          220 c          237 g          421 t
ORIGIN      
        1 ctggtttttt aacggttcat taatggtaag ttcacaagta ttgtccgatc gttgaataat
       61 ttgggcgtta taacctaaaa aatccagacg aagagacgag tcattataaa gaattacatt
      121 ttgtaagtac gctaattctt tttgaaaatc accagtgata atgcattttc cactaggttg
      181 taacgtgtta agtaaagtga ccatttcaat agaggtctgt tttagttttt caatacctgg
      241 tgctagtgct tcgaggttat ttcctgttgg gctttcaggt aggtcattca tttttcgacg
      301 gactaaatca ctactaatat cattaccaat tgatgaaaca gggaacatta ttttatcctt
      361 tataaataat caataaaaat cattattaaa atgatcgggt gaaaaaaaga gaatttgcca
      421 acaatctgaa gtaatactgg ttttattaaa gtacgcaggg ggtgttaaga tggcttcttt
      481 tcgggtgaat tttgcacaat acttagaaat cgtctcctta atacattcca gctctttttt
      541 attgacggct gagcaagtac atttttcgcc aaaaggtgca aaagtgccta catctaatgt
      601 ttgtatgata taatcgtcat gttctcctgt agaaatatct atgatgaatg gactcatatt
      661 ttccggttgg attcggatca tgacagaagg aacatcgcct tgatagaaat tcaatgcagt
      721 aattgcagat aatactctcc gcagttcatt acttatactg taaattttat taacgtcatt
      781 ggtggagaca atttctgcaa actttctttt taccgtccaa taatcaatat ttctattgtg
      841 attgttaacc actgcgtgca ttggtgggat tgaagggtga aaaatatgtg caattgggaa
      901 gcctttcatt gatgcctgcc ttgtcatcat gacaaacaat aatagatgct tattttcctc
      961 gcagggttct ttcgcactat caaaaaacgc tcatttaccc ggcgttacag taaacgaagc
     1021 aacattctgt ggcctacgcc gatcaaaagc tctgtgaaaa tttcccttgt tgtctttgtc
     1081 tgaccttatc gcggcgatca gcaattattc gttttgtgat cctcaacaca aaatgctatt
     1141 cgtcgcctat gaaggaagaa gaatttatgg aaaacgattg cattcacaaa atgacgtcct
     1201 gtcagaagag aaaaagttga cgttaatcaa ttgttcgaga tgatcaatac ctgtattcga
     1261 aat
//
LOCUS       DAZFAB010000302         1250 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZFAB010000302 DAZFAB010000000
VERSION     DAZFAB010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.306"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..1176
                     /locus_tag="ACHJWA_005064"
     CDS             <1..1176
                     /locus_tag="ACHJWA_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO4311464.1"
                     /translation="RRTGKRVWRRGRDLTGWMSLSRKPEVTWYGWDGDRLTTVQTDTT
                     RIQTVYEPGSFAPLIRIETDNGEREKAQRRSLAEKLQQEGSEDGHGVVFPAELVRLLD
                     RLEEEIRADRVSSESRAWLAQCGLTVEQLARQVEPEYTPARKVHLYHCDHRGLPLALI
                     SEDGNTAWSGEYDEWGNQLNEENPHHLHQPYRLPGQQYDKESGLYYNRNRYYDPLQGR
                     YITQDPIGLEGGWSLYAYPLNPVNGIDPLGLSPADVALIRRKDQLNHQRAWDILSDTY
                     EDMKRLNLGGTDQFFHCMAFCRVSKLNDAGVSRSAKGLGYEKEIRDYGLNLFGMYGRK
                     VKLSHSEMIEDNKKDLAVNDHGLTCPSTTDCSDRCSDYINPEHKETIKALQDAGYLK"
     gene            1157..>1250
                     /locus_tag="ACHJWA_005065"
     CDS             1157..>1250
                     /locus_tag="ACHJWA_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311465.1"
                     /translation="MLAISSNLSKMIIFIIAIIIIVVLCVITYLY"
BASE COUNT          344 a          254 c          385 g          267 t
ORIGIN      
        1 cgccggacgg ggaaacgggt gtggcgtcgc gggcgtgacc tgacgggatg gatgtcgctg
       61 tcgcggaaac cggaggtgac gtggtacggg tgggacggcg accggctgac gacggtacag
      121 accgacacca cgcgtatcca gacggtatac gagccgggaa gcttcgcgcc gctcatccgc
      181 atcgaaacag acaacggtga gcgggagaaa gcgcagcgcc gcagcctggc ggagaagctg
      241 cagcaggaag ggagcgagga cgggcacggt gtggtatttc cggctgaact ggtgcggctg
      301 ctggacagac tggaggaaga aatccgggca gaccgcgtga gcagtgaaag ccgggcgtgg
      361 cttgcgcagt gcgggctgac ggtggagcaa ctggccagac aggtggagcc ggaatacaca
      421 ccggcgagaa aagttcatct ttaccactgc gaccaccggg gcctgccgct ggcgctcatc
      481 agcgaagacg gcaatacggc gtggagcggg gagtatgatg aatggggcaa ccagctgaat
      541 gaggagaacc cgcatcacct gcaccagccg taccggctgc cggggcagca gtatgataag
      601 gagtcggggc tgtactacaa ccggaaccgg tactacgatc cgttgcaggg gcggtatatc
      661 actcaggacc cgatagggct ggagggggga tggagtctgt atgcgtatcc gctgaatccg
      721 gtgaatggta ttgatccatt agggttaagt cccgcagatg tagcgctaat aagaagaaaa
      781 gatcaactaa accatcaaag agcatgggat atattatctg atacttatga agatatgaag
      841 agattaaatt taggtgggac tgatcaattt ttccattgta tggcattttg tcgagtgtct
      901 aaattaaatg acgctggtgt tagccgatcg gcgaaagggc tgggttatga aaaagagatt
      961 agagattacg ggttaaatct gttcggtatg tacggcagaa aagtaaagct atcccattct
     1021 gaaatgattg aagataataa aaaagacttg gctgtaaatg accatgggtt gacatgtcca
     1081 tcaacaacag attgctcaga tagatgtagt gattatatta atccagagca taaagaaacg
     1141 ataaaggctt tacaagatgc tggctatctc aagtaatcta tcaaagatga taatatttat
     1201 tattgctatt ataatcattg ttgttttatg cgtaattact tatctttatt
//
LOCUS       DAZFAB010000303         1233 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZFAB010000303 DAZFAB010000000
VERSION     DAZFAB010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.307"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..>1233)
                     /locus_tag="ACHJWA_005066"
     CDS             complement(<1..>1233)
                     /locus_tag="ACHJWA_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO4311466.1"
                     /translation="NHQDKHSDKVNNHQHHGKVDKKHRSEIVEIDKLDKHSQIDNDFG
                     LHIIYFLQHGCWNVTEHSPQIEKIWFYNSEPSIDIKEYNSFADNTTDTFIFTIIPDNN
                     HVIKLSSPITVTIERKDGYYFINFSGDKSDIIYKVDGFSIKERNFFTLLSGNFKPDWR
                     WAVSKETFTKEKFDSYVESVFSKIDFYKQCGVINPQNANTAYFGDTDGRVGAVLYALL
                     VSGHIGIREKGWSLLCDLLKHEDMASFAYENKKLKKLFTLLDKRDMILNELHQHVFLK
                     GDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRINKNVVVLAGNHEI
                     NFNGNYSARLANHKLSSGDTYNLIKTLDVCNYDSERHVLTSHHGIIRDEENKCYCLGA
                     LQVPFNQMKNPTDPEELAY"
BASE COUNT          389 a          234 c          191 g          419 t
ORIGIN      
        1 atatgctaat tcctcaggat cagtaggatt tttcatctga ttaaatggaa cctgcaaagc
       61 accgagacaa taacatttat tttcttcatc ccttattatt ccgtgatgac tggttaaaac
      121 atgcctttct gagtcataat tacaaacatc gagcgtttta attaagttat atgtatcacc
      181 ggaagagagt ttatgatttg ctagtcgtgc actatagtta ccattaaaat tgatttcatg
      241 atttccagcc agaacaacga catttttatt tatacgacta tctttattac cctccatatt
      301 tcgcatagaa tttagcaaag taaggatata tttatcacca aaaatagtac tgaatctatc
      361 gcctgtatga tccccaagga agatacaagg ggtaatcgca tcgcctttta aaaaaacatg
      421 ttgatgcaac tcattcaaga tcatgtctct cttatctaat aaagtgaaga gttttttcaa
      481 ttttttattt tcatatgcga atgaagccat gtcttcgtgt ttcaataaat cacataataa
      541 actccacccc ttttccctga tgcctatatg ccctgaaacg agcaatgcat aaagtacagc
      601 gcctactctt ccatctgtat cgccaaaata agccgtgttg gcattttgtg ggttaatcac
      661 accacactgt ttataaaagt ctatttttga aaaaacagat tcaacatagc tatcaaactt
      721 ttccttagta aacgtctctt tagagacagc ccaacgccag tcaggtttga aattaccact
      781 aagaagagta aagaaatttc tctctttgat agaaaaccca tcaactttat aaattatgtc
      841 tgatttatcc ccggagaaat taataaaata ataaccgtcc ttacgctcaa tcgtaacggt
      901 aatgggagac gataatttta tcacatggtt attgtctggt ataattgtaa agataaacgt
      961 gtcagtagta ttgtcggcga aactattata ttctttaata tctatagatg gctcactatt
     1021 gtaaaaccat attttctcta tctgaggact atgttcagtt acgttccagc agccatgttg
     1081 caggaaatag atgatatgca ggccaaaatc gttatctatt tgcgaatgtt tatccagctt
     1141 atcaatttca actatctctg aacgatgttt tttatcaacc ttgccatggt gttgatgatt
     1201 attaacttta tctgaatgct tatcttgatg gtt
//
LOCUS       DAZFAB010000304         1231 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZFAB010000304 DAZFAB010000000
VERSION     DAZFAB010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.308"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            84..1229
                     /gene="espG"
                     /locus_tag="ACHJWA_005067"
     CDS             84..1229
                     /gene="espG"
                     /locus_tag="ACHJWA_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspG"
                     /protein_id="HFO4311467.1"
                     /translation="MINGLNNDSASLVLDAAMKVNSGFKKSWDEMSCAEKLFKVLSFG
                     LWNPTYSRSERQSFQELLTVLEPVYPLPNELGRVSARFSDGSSLRISVTNSELVEAEI
                     RTANNEKITVLLESNEQNRLLQSLPIDRHMPYIQVHRALSEMDLTDTTSMRNLLGFTS
                     KLSTTLIPHNAQTDPLSGPTPFSSIFMDTCRGLGNAKLSLNGVDIPANAQKLLRDALG
                     LKDTHSSPTRNVIDHGISRHDAEQIARESSGSDKQKAEVVEFLCHPEAATAICSAFYQ
                     SFNVPALTLTHERISKASEYNAERSLDTPNACINISISQSSDGNIYVTSHTGVLIMAP
                     EDRPNEMGMLTNRTSYEVPQGVKCIIDEMVSALQPRYAASETYLQNT"
BASE COUNT          390 a          258 c          239 g          344 t
ORIGIN      
        1 tcttgtatat tattgacact atatgatgct atatgatact tgttgccaaa ttgttcatta
       61 caaaccagat aggagaatct ctcatgataa atggacttaa taatgactcc gcatctttag
      121 ttttagatgc tgcaatgaaa gttaattctg ggtttaaaaa aagctgggat gagatgtcat
      181 gcgctgaaaa gttatttaaa gtacttagtt ttggtttatg gaatccaacg tacagtcgta
      241 gtgaaagaca atcatttcaa gagttgttaa ccgttttaga gcctgtatat ccacttccca
      301 atgaattagg cagagtatct gctcgttttt cagatggttc atccttaaga atttccgtca
      361 ctaacagcga acttgttgaa gccgagattc gcacagcaaa taatgaaaag attactgtgc
      421 tcctggagtc aaacgaacaa aataggttat tacaatcttt acccatcgat cgccacatgc
      481 catacattca ggttcatcgt gccttatctg agatggacct gactgatact acctcaatgc
      541 gcaatctact tggttttacg tcaaaactat caacaacctt gattcctcat aatgctcaaa
      601 cagatccgct ttccgggcct acaccattca gctctatctt tatggataca tgtcgaggac
      661 tcggcaatgc aaagctttca ctcaatggtg ttgatatacc tgcaaatgca caaaaattgc
      721 ttcgcgatgc actaggactt aaagacacac attcatcacc aacccggaat gttatagatc
      781 atggtatttc tcgccatgat gcagagcaaa tagcaagaga aagcagcggc agtgataaac
      841 agaaagctga agttgtggaa tttttatgcc atccagaagc agcaacggcc atatgctcgg
      901 ctttctatca atctttcaat gtgccagcct taacgttgac acatgaaagg atctctaaag
      961 ccagtgaata caatgcggaa agatcattag atacacctaa cgcttgcatt aacatcagta
     1021 tctctcaatc atcagatgga aacatttatg ttaccagcca tactggggtt ctgataatgg
     1081 cgccagaaga ccgccccaac gagatgggca tgttgacgaa caggacttct tatgaagtgc
     1141 cgcaaggtgt gaaatgtata atcgatgaaa tggtaagtgc gctacaacca aggtatgccg
     1201 catctgaaac gtacttacaa aacacttaaa a
//
LOCUS       DAZFAB010000305         1214 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZFAB010000305 DAZFAB010000000
VERSION     DAZFAB010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.309"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            139..246
                     /gene="yniD"
                     /locus_tag="ACHJWA_005068"
     CDS             139..246
                     /gene="yniD"
                     /locus_tag="ACHJWA_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFO4311468.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(299..1057)
                     /locus_tag="ACHJWA_005069"
     CDS             complement(299..1057)
                     /locus_tag="ACHJWA_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFO4311469.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
BASE COUNT          340 a          289 c          282 g          303 t
ORIGIN      
        1 tttatttttt cataaaacgc agaaacagtg atcaggtgta caacgccacc cgacggctgt
       61 aataacaaaa aatcccttga tgcctgtccc ttttgttaca ctccgttatc acgcacaaga
      121 gatatgcagg acactggtat gccgactaaa cgctttgata aaaaacactg gaagatggtg
      181 gtggtgctac tggcaatctg tggcgctatg ttgttgctac gttgggcagc aatgatttgg
      241 ggctgagcag tgacaaacga agacagccag accgcataac acggtccggc agataatttt
      301 acatgctgta gcccagcgat aaggttgtcc gacggtcggt atgttccggt gcagactctg
      361 gaggttcaga gttccatgtg acgttgtaag ccaccttcag tccaaaatgc tcattaatag
      421 caacatttaa tgcgctttcg gagttcagcg ttgtgtcttc cgcgccaaag acggaaacac
      481 cctgcgtaaa tttagcgttg tcagtaagct gccatgcata agcaccggag gcatagccca
      541 gcggctgagt ttcactggca ttgtcggtat atttgtcgta acgcacacct ggaccgaatt
      601 caaagcggaa actgtgtacc gggccattca aaaactgacg accataaccc gcggtcaaca
      661 catcgcgctc tcgatagccg ttataacggt ctgtcagcca gcttgcctga ccaaataaat
      721 agtcataatc agttaaatta aaacggctac gcccgcccgc cgcatatttt tctgaagaac
      781 gctcatcatt agaagaagta ttactggcgt tcccccacag cgaccaggca gtggtttgtc
      841 cataccaggt catggtggtg tcagccgtaa gtgaggagct tttcgtattg cctgattgtg
      901 caagataccc tgcgttcaga ttaccttcga aaggtttttt agcgctggca ggatcgtcca
      961 tgacagtaaa aacggaatca tcggcagctg cattcagtga cgcaaacatg ccccccgcca
     1021 acataacgat ggcaggaact gtcttcaaaa gcttcattta tcaagagtcc gtacaacaaa
     1081 aaaagagacc atcgcggtcc cggaaacttt cttaaggatc aaagattagc gtccctggaa
     1141 aggtaacgaa ttataaaaag gcgcgaataa cttatcaatg tattcttatt tcattttttg
     1201 aataagcatg tggc
//
LOCUS       DAZFAB010000306         1207 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZFAB010000306 DAZFAB010000000
VERSION     DAZFAB010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.310"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(134..708)
                     /locus_tag="ACHJWA_005070"
                     /pseudo
     CDS             complement(134..708)
                     /locus_tag="ACHJWA_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131658.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
     gene            complement(821..1090)
                     /locus_tag="ACHJWA_005071"
     CDS             complement(821..1090)
                     /locus_tag="ACHJWA_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO4311470.1"
                     /translation="MNILKKLMQRLCGHGKHDDREDGELLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
     gene            complement(1092..>1207)
                     /locus_tag="ACHJWA_005072"
     CDS             complement(1092..>1207)
                     /locus_tag="ACHJWA_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO4311471.1"
                     /translation="TRVQGFFQHLQVRFGDGPWQDVKGLDEVGSDTGRTGE"
BASE COUNT          337 a          319 c          209 g          342 t
ORIGIN      
        1 aaaatgcatt tacataaaga cgccttcaag tcatctgaaa cctgccaacc acagacacct
       61 tcttatattc tggatgtctg cggcgttttt ttacaaatta ctgacagcca atactctctc
      121 agagaaaaat attttaacta tcttttataa tgaagtttcc cttgatagga tcataaacac
      181 atttatcagg acttataatc attgatgccg ttattggttc tcgggtcagt ggatgatatg
      241 agccttcact agctaaacga gaaaatgcat caaaatcaaa taagcagcat actgccgaac
      301 tatctgaatt tttgacaaac actccttctt cgggtctctc tagtgtaatt ggacactgaa
      361 tagactctgg cgggcactgg aaatttcctg aactaacagg aaacttacat tgagatattt
      421 tgtcctgaat actctcttgc gattgtgctt cacctgagtc cgaaagcata ctgagcatcc
      481 tatcgcgaag agcttcaggg ccgttattaa acccataaag gaataactca gtaagcaatc
      541 ccccactact gccgctggat agaaaacgcc catcggtttc attaaataca atgctgacag
      601 tctcattacc taatgtaaac tcccctcgac cgctagatgc tacttgagct cgaaggacat
      661 tcaatccatt aaaaatgagc ttgatctaat ctccgaattt aacggcatta taaaactccc
      721 tattttaatt taaactccag acttaaatag ctttaacaaa catctgcctt acatgacaac
      781 aaaaaccgga gccggactcc ggttttgtga agctgtcggg ttacttcatc ccgccaatat
      841 tttcccacgt cccgtcagca cgcaggattt gcagcggtct taccacgcac tgtatctgct
      901 ttttatccgc atccagtatc accacctgcg tgattaccct gtcctgctcc ggaataatac
      961 cattctcatc ggactccagg atgtctgccg gccccagacg cagttgtgct gtaagtaact
     1021 ccccgtcttc acggtcatca tgctttccgt gcccgcacag acgctgcata agttttttta
     1081 gtatgttcat gtcattctcc tgttctgcct gtatcactgc ccacttcatc aagcccctta
     1141 acatcctgcc acggcccgtc accaaacctg acctgcaaat gctgaaaaaa accctgaacc
     1201 cgtgtgg
//
LOCUS       DAZFAB010000307         1195 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZFAB010000307 DAZFAB010000000
VERSION     DAZFAB010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.311"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            120..272
                     /locus_tag="ACHJWA_005073"
     CDS             120..272
                     /locus_tag="ACHJWA_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO4311472.1"
                     /translation="MDMLNLGNNESLVCGVFPNHDGTFTAMTYTRSKTFKTEAGAHRW
                     LARNAN"
     gene            753..941
                     /gene="dicB"
                     /locus_tag="ACHJWA_005074"
     CDS             753..941
                     /gene="dicB"
                     /locus_tag="ACHJWA_005074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO4311473.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNKAFTEDAINMRKYEPYLLNDN
                     SILSRIALIKLGIFGERQ"
     gene            938..1126
                     /locus_tag="ACHJWA_005075"
     CDS             938..1126
                     /locus_tag="ACHJWA_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO4311474.1"
                     /translation="MTSAFALVMTVFLITGESQNVITGIYASKESCLQARDEQKISGE
                     CLPVKKVSLYLNNETPAG"
BASE COUNT          333 a          264 c          290 g          308 t
ORIGIN      
        1 gtggtcaggg gttaagtagc cagcccgagg cgtatgaaca tgacggcggg aacactttgt
       61 ataacagcgc agcaggtttt tagttccgcg acccggcgtt aagggtaaat gaggtcaaca
      121 tggatatgct caatcttggc aacaatgaat ctctggtgtg cggagtattc cccaaccacg
      181 acggcacgtt taccgcgatg acgtatacca gaagtaaaac gtttaaaacc gaagctggcg
      241 cgcatcgctg gttagcaaga aacgctaact gattagcgcc agtaaaaaca ggtttccaca
      301 ggttaattta ccctgaaaag tcagggcata acacgaaagc gcacggcgaa gttagtctct
      361 ctgtataggt cgtcgttaaa tttaattcga ccgtgcgctt ctggttgtgg caatccgcga
      421 aatggcgcgg cggtaagtat ggcggggtta ttccttcccc cgttgaggac accgggttgt
      481 caggttgacc atacgcttaa gtgacacccc agccacaaca acctctgtta tcacttttct
      541 ggtgattcgg cggaaatgga tatccgccct ttttaaagtg aattttgtga tgcggtgaat
      601 gcggctatgc gcacgcggaa cagttaaagc agtaaggcgg tcttttactg gcgtaacgag
      661 catcaactaa cccgacgtta attgttaact ggttaacgtc acctggaggc accaggcact
      721 gcatcacaaa attcattgtt gaggacgcga taatggaaac gttattacca aacgttaata
      781 cgtctgaagg ttgttttgat attggtgttc tgctcagtaa taaagcgttt actgaggatg
      841 ccattaatat gcggaaatat gagccttatc tgctcaatga taattccatt ctttcccgaa
      901 ttgcccttat taaacttggt attttcggag aacgtcaatg acttcagcat ttgcactggt
      961 gatgacggtt tttcttataa cgggtgaatc acagaatgtt attaccggaa tttatgccag
     1021 taaagaatcc tgcctccagg caagagacga gcaaaaaatt tctggtgaat gcctaccggt
     1081 aaaaaaagta tcgctgtacc tgaataacga aacaccggct ggataacccg ccagccatat
     1141 taacgccata ccaacggatt aaaaatgcca gcaatggcag ggattcgttc accct
//
LOCUS       DAZFAB010000308         1189 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZFAB010000308 DAZFAB010000000
VERSION     DAZFAB010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.312"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..>1189)
                     /locus_tag="ACHJWA_005076"
     CDS             complement(<1..>1189)
                     /locus_tag="ACHJWA_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO4311475.1"
                     /translation="VVKGVSFMLRLTVVADDGSERLVSTARTTETTYRFTQLALGNYR
                     LTVRAVNAWGQQGDPASVSFRIAAPAAPSQIELTPGYFQITAVPRLAVYDPTVQFEFW
                     FSETRITDIRQVETTARYLGTGLYWIAASINIKPGHDYYFYIRSVNTVGKSAFVEAVG
                     QPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVSNEITQTVN
                     KKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIENTPAGMQS
                     QVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNPPAFSLTP
                     DGKLTAKNADISGSVNANAGTLNNVTINENCQIKGKLSANQIEGDIVKTVGKAFPRDS
                     RAP"
BASE COUNT          248 a          355 c          283 g          303 t
ORIGIN      
        1 tccggtgccc gggagtcccg cggaaaagcc ttacccactg ttttgactat atcgccttca
       61 atctggttgg ctgacagttt ccccttaatc tgacagttct cattaattgt gacattgttg
      121 agcgtcccgg cgttcgcatt cacactgcca ctgatatctg catttttagc ggtcagcttt
      181 ccgtccggtg tcagggaaaa tgccggagga ttaccgccgc tggtaatggt gggagccgtc
      241 aggcgtttca ggaacacgtc gttcatgaat atctgatcgc cctgaccaac aaacatcggc
      301 tttgtgttgc cattcgcagg attaatcatc gcaatcctgt ctgccgccag cagcacctga
      361 ctctgcattc ctgctggcgt attctcaata ccggcaccga tacccgcaat ataaaggcgt
      421 ccgtccttca tctgttgcag cttcacagcc cacatgctgt tcagattatt atttgtatca
      481 acctgaacct tctgtatctg ctggatcgct gcactctggt cttccagttt cttattgacg
      541 gtctgcgtga tttcattact gacatccgtg atggacgttc tgatttccgc caggtcaggc
      601 gcaagctgac cgttatcaat ctgagtccac aactcctgag ccagatgggt tttccctatc
      661 tctcctttga aaaaatccag atagccggat gcatcatcac tcggctggcc aacagcctcc
      721 acaaatgccg atttgccaac ggtgttcaca ctgcggatat aaaagtaata atcatggccc
      781 ggtttgatat tgatactggc ggctatccag tacagccccg tgccaaggta gcgggctgtg
      841 gtttcaacct gcctgatatc ggtaatccgc gtttccgaaa accagaactc aaactgtacc
      901 gtcgggtcat acaccgcaag acgcgggacc gctgttatct gaaaatagcc cggtgtcagc
      961 tcaatctgtg acggcgctgc cggtgcggca atccggaacg ataccgacgc cggatcgccc
     1021 tgctgccccc acgcatttac cgcccggact gtcagcctgt agttccccag cgccagttgc
     1081 gtgaagcggt atgtggtttc cgtcgtccgg gccgtgctga ccagccgctc actgccgtca
     1141 tccgcgacca cggtcaggcg aagcataaag ctcacgccct tcaccaccc
//
LOCUS       DAZFAB010000309         1188 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZFAB010000309 DAZFAB010000000
VERSION     DAZFAB010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.313"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(1..137)
                     /locus_tag="ACHJWA_005077"
                     /pseudo
     CDS             complement(1..137)
                     /locus_tag="ACHJWA_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            287..955
                     /locus_tag="ACHJWA_005078"
     CDS             287..955
                     /locus_tag="ACHJWA_005078"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO4311476.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
BASE COUNT          357 a          250 c          223 g          358 t
ORIGIN      
        1 gctactgatg tatcaacacg attcaacaat acccgatatt tattccatgc ctccagcaac
       61 gatctttctt cctccgttgc gatttccaga tctacagcat cctgcagtgg cgcaatatac
      121 tcactgaatt cctggatgta gaactgtgtg gtgacggtct tccagccatt cggctcctgc
      181 tgtatcgcag cataccaggc tatttcaata tcgctatgct gcggcagcat ttaacccctt
      241 gtaattcatc gccataattg atttaattca caaataaaac tataacatgg tgaaattaat
      301 gaaaaaaaac acagatgatg gggctaaaat ttacacacca cttaccctaa agctttatga
      361 ctggtgggtt ttgggagtat caaatcgtct tgcatgggga tgtcctacaa aggaacacct
      421 tcttccacac tttctggaac atttaggtaa caaccatctg gatattggtg ttggaactgg
      481 gttttacctt actcacgtac ctgagagtag tctgatatct ttaatggatt tgaacgaagc
      541 tagcctgaac gcggcatcta caagggctgg ggaatcaaaa attaaacata aaattagcca
      601 tgatgttttt gaaccttatc ccgcggcgtt acatggtcaa tttgattcca tttccatgtt
      661 ttaccttctt cactgcctgc ctggaaatat atctacaaaa agctgtgtaa tacgcaatgc
      721 ggcgcaggcc ttaactgacg atggaactct atacggagcc acaattcttg gcgatggagt
      781 tgtgcacaat agcttcggtc aaaaactgat gcgcatttac aatcagaaag gcatcttttc
      841 aaacacaaaa gattccgaag aaggcttaac acatatactc tcagagcatt tcgagaatgt
      901 taaaaccaag gttcaaggta ctgtagtaat gttttccgct tcagggaaaa aatagcatcc
      961 aaccgcagca cgttcttgca tacgccgtgt ctgcggttta atttttactc tcaaatattg
     1021 tagttaagta caataatgat tcctgtggct atatagatgt ttttgtaatt agtccttaag
     1081 ataaaatttc tctatttatc caagaaacag acttcattag gaagagtcgt aaaaacatta
     1141 acaactaccg ccgtgtaatt aaattatttt ttagaatgtt ctgattga
//
LOCUS       DAZFAB010000310         1185 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZFAB010000310 DAZFAB010000000
VERSION     DAZFAB010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.314"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            54..644
                     /gene="espM2"
                     /locus_tag="ACHJWA_005079"
     CDS             54..644
                     /gene="espM2"
                     /locus_tag="ACHJWA_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector guanine nucleotide exchange factor
                     EspM2"
                     /protein_id="HFO4311477.1"
                     /translation="MPMNTTGMSFSSFGISCHRENSFRNSFRGKNDEVVKCSIGERTI
                     SFSVRKFSGNILETVRRQSTKDIDEWIKDERIVYPSRVINQEIDNYCFQKNAKISTEE
                     RQRVFFLVSQENQLTLDVKAAQSSINHVIMGSASFGKKMDALCDGMSRDVKNRTSDTI
                     ANLLADKFYQKHIDSDIDIVKLRNDIPDYLMRAIQG"
     gene            878..1030
                     /locus_tag="ACHJWA_005080"
                     /pseudo
     CDS             878..1030
                     /locus_tag="ACHJWA_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885624.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
BASE COUNT          386 a          213 c          274 g          312 t
ORIGIN      
        1 aataaacgca agtatgcaat attgtaaaca cttttttata aaaaggaatt ataatgccga
       61 tgaatactac aggtatgtct ttcagctctt ttggtataag ttgccataga gagaactcct
      121 tcaggaactc tttccgaggg aagaatgatg aggtcgtaaa atgttcaatt ggagaacgga
      181 ctattagctt ttctgttcgc aaatttagcg gcaatatact ggaaacggtg agaaggcaga
      241 gtactaaaga tattgatgag tggataaaag atgaacggat agtatatccc tcaagggtga
      301 tcaaccagga gattgataat tactgttttc aaaaaaatgc aaaaatttcc actgaagaga
      361 ggcaaagagt cttttttctt gtgagccagg agaatcaact aacccttgat gttaaggcag
      421 cacaaagttc tattaatcat gtaataatgg ggagtgcttc ttttggcaag aaaatggatg
      481 ctctttgtga tggtatgagc cgggatgtaa aaaatcgtac atcagatacc atagcaaact
      541 tgcttgcgga caagttttac cagaaacata tcgattcgga tattgatatt gtaaaactac
      601 gaaatgatat cccagattat ttgatgcgtg ctatacaggg atgaggctag aggagaggac
      661 tgcttcagca tcatcggctt cggaccagta aaagagacgc agaaaatgca caacggcacc
      721 acacgccatg catggattca gattactcat ggctcaccag tacagctata aatcgtaaag
      781 aaaactacag tacgttatac acagaaacgt accgtgattc attatttatt gtacaggttc
      841 atcgcagcga caatattttg tgatgtttgc gtggtgcgcc ccacttcagg atgctgcaga
      901 tctggaaatt gcaacggagg aagaaacctc attgctggaa gcctagaaaa agtatcgggt
      961 gttgctgaac cgtgttgata catcaactgc acctgatatt gagtggccgg aagaaccaga
     1021 cacaatgtaa gcgaaaaaga aaaaccgcag acacgacgta tgcaggacgt gctgcggttg
     1081 gctggtgaac tttcgatagt gcgagtattg aatgatttcc agccgttacc gattttacgt
     1141 gttaattagt gaacaaacca ctcgtcagca gacttccagg tatct
//
LOCUS       DAZFAB010000311         1183 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZFAB010000311 DAZFAB010000000
VERSION     DAZFAB010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.315"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            2..>1183
                     /locus_tag="ACHJWA_005081"
     CDS             2..>1183
                     /locus_tag="ACHJWA_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5507 domain-containing protein"
                     /protein_id="HFO4311478.1"
                     /translation="MNKSNGFNASAVWGSGSYNENKSSKHMELLAHSIVKLICKEAAS
                     ETYRGALEILQKIMSECIYQEGNAFVIMGAGEQLKRIKYDVDENNLKVFNVHFDNNEV
                     LVTDGEPDVVCLSKQVWENLLIKLKPEIKENVASEVHKSANKGEIEQLVEWSKRNEQT
                     LFDNIIKSDFHVGSLKPGSMNGVILEMPPNVCMEPRNSYENKIDEVSSLSESEEHPID
                     IQKITDAFVKEFKGILFDKNGRSSELLFNFYECCYTFLPRAQPQDKIDSYNSALQAFS
                     IFCSSTLTHNNVGFDFKLFPEVKLSGEHLETVFKYKNGDDVREIAKINITLQKEEGGL
                     YNLRGLDFKGCFFSGQNFSNYDIQYVNWGMSLFDVDTPCIFNTPANHESYEKSLKPVS
                     EN"
BASE COUNT          414 a          168 c          240 g          361 t
ORIGIN      
        1 aattaataaa agcaacggat ttaacgctag cgcagtttgg ggtagtggaa gttataatga
       61 aaataaatct tctaaacaca tggagctact agctcatagt attgtaaaat taatttgtaa
      121 ggaagctgca tcagagacgt atcgcggtgc tcttgaaatt ttacaaaaaa taatgtctga
      181 atgtatatat caagaaggca acgcctttgt cattatggga gctggggaac aattaaaacg
      241 tattaaatat gatgttgatg aaaataactt aaaggtattc aacgtacact ttgataataa
      301 tgaagtgtta gttactgatg gtgagcctga cgtagtatgt ttaagcaagc aggtctggga
      361 aaatcttctc attaaattaa aaccggagat caaggaaaat gtggcttctg aagttcataa
      421 atcagcgaat aaaggtgaga ttgagcaatt agttgaatgg tctaaaagaa atgaacaaac
      481 ccttttcgat aatataataa aaagtgattt tcatgttggt tcacttaagc caggtagtat
      541 gaatggtgtt attttagaaa tgccaccaaa tgtctgtatg gaaccacgta attcatatga
      601 aaacaaaata gatgaggttt catctttgtc agagtcagag gaacacccca tagatattca
      661 aaaaataaca gatgcgtttg tgaaggagtt caaggggata ttatttgata aaaatggaag
      721 atcttcagag cttctgttta atttttatga atgttgctat acgtttttac caagagcgca
      781 gcctcaagat aaaatcgata gctataattc agcactgcaa gctttttcca tcttttgttc
      841 atctacgttg acacataata atgtaggctt tgatttcaaa ttatttccag aagtcaagct
      901 gtctggagaa catcttgaaa cggtattcaa atacaaaaat ggcgatgatg tccgggagat
      961 agccaaaatt aacattactc tccaaaaaga agagggtggc ttatataatc tacgtggatt
     1021 ggattttaag ggatgcttct tttctggaca gaacttcagt aactatgata ttcaatatgt
     1081 gaactgggga atgtcattgt ttgatgttga tactccgtgt atttttaata cgcctgctaa
     1141 ccatgagagt tatgaaaaat cattaaaacc tgtaagcgaa aac
//
LOCUS       DAZFAB010000312         1180 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZFAB010000312 DAZFAB010000000
VERSION     DAZFAB010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.316"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..253
                     /locus_tag="ACHJWA_005082"
     CDS             <1..253
                     /locus_tag="ACHJWA_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO4311479.1"
                     /translation="SGAVIRGVFDDPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRG
                     DTLTINGEMFWVDRVSPDDGGSCYLWLNRGQPPAVNRRR"
     gene            268..801
                     /locus_tag="ACHJWA_005083"
     CDS             268..801
                     /locus_tag="ACHJWA_005083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4311480.1"
                     /translation="MAIKGLDQAIENLSRVRKNAIPAASAMAINRVATTAINQSSSQV
                     ARETKVRRKLVKERSRLKRATVRNPNARIIVKRGDLPVIKLGIRMLGRRPDSILKAGQ
                     HRYQRAFIQRLKNGRWHVMQRVAGKNRYPIDVVKIPMAAPLKQAFDENIDRIRRERLP
                     GELAYALKQQLRIAIKR"
     gene            798..>1180
                     /locus_tag="ACHJWA_005084"
     CDS             798..>1180
                     /locus_tag="ACHJWA_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO4311481.1"
                     /translation="MKHTDIRAAVLDALELHEHGATLFDGRPVVFDEEDFPAVAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDQWMESRIYPAVTAIPALADLITTM
                     VTQGYEYRRDDDMALWSSADLTYSIT"
BASE COUNT          280 a          274 c          359 g          267 t
ORIGIN      
        1 gtccggcgca gtaatacgtg gtgtttttga tgaccctgaa aatatcagct atgccggaca
       61 gggcgtgcgc gttgaaggct ccagcccgtc cctgtttgtc cggactgatg atgtgcggca
      121 actgcggcgt ggtgacacgc tgaccattaa cggcgagatg ttctgggtgg accgtgtttc
      181 tccggatgac ggagggagtt gttatctctg gctcaaccgt gggcaaccac cggcagttaa
      241 ccggcgacga taaacgcagg gtgaattatg gcgataaaag ggcttgatca ggcgattgaa
      301 aatctgagcc gggttcgtaa aaacgccatt ccggcggctt cagcaatggc cattaaccgc
      361 gtggccacaa cggcgattaa tcagtcttca tcacaggttg cccgggagac aaaggtacgc
      421 cggaaactgg taaaggaacg gtccagactg aaacgggcca cggtcagaaa tccgaatgcc
      481 agaattatcg ttaaacgcgg tgatctccct gtgattaagc tggggatcag gatgctgggc
      541 cgtcgtccgg acagcatact taaagctggt cagcatcggt atcagcgggc atttatccag
      601 cgattaaaaa atggtcgctg gcatgtcatg cagcgtgtgg ccgggaaaaa ccgttacccc
      661 attgatgtgg tgaaaatccc gatggcggcc ccactgaaac aggcgtttga tgagaatatt
      721 gaccgtatcc ggcgtgaacg tctgcccgga gaactggcat acgcgctgaa acaacaactt
      781 aggattgcga taaaacgatg aaacatactg atatccgtgc ggcagtgctg gatgcactgg
      841 agctgcatga acacggggcg acgctgtttg atggtcgccc cgttgttttt gacgaagagg
      901 attttccggc cgtcgcggtt tatctgacgg atgcagagta taccggtgaa gagctggatg
      961 cagatacctg gcgggccaca ctgcatattg aggtgttttt accggcacag gtaccggatt
     1021 cggagcttga tcagtggatg gaaagccgga tttatccggc ggtgactgcg atcccggcac
     1081 tggcagacct gattacgacg atggttacgc agggctatga gtatcgtcgt gatgacgata
     1141 tggcgttatg gagttctgcg gatctgactt attccattac
//
LOCUS       DAZFAB010000313         1176 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZFAB010000313 DAZFAB010000000
VERSION     DAZFAB010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.317"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..251
                     /locus_tag="ACHJWA_005085"
     CDS             <1..251
                     /locus_tag="ACHJWA_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO4311482.1"
                     /translation="QFFRLLNRRYEKASIILTSNKGFADWGEMFGEHVLATAILDRLL
                     HHSTTLNIKGESYRFNIPDRLVVHLSLHARPELRAHNT"
     gene            complement(244..468)
                     /locus_tag="ACHJWA_005086"
                     /pseudo
     CDS             complement(244..468)
                     /locus_tag="ACHJWA_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(542..>1176)
                     /locus_tag="ACHJWA_005087"
     CDS             complement(542..>1176)
                     /locus_tag="ACHJWA_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311483.1"
                     /translation="IDNKLSDNRINITPMGIKIQETVSGNDRLFFYPTPLKNGCFILH
                     NPLYSNSFPSLYSHELFDLIEAYRNPTYSYLQNNIINRYIHTVSEYAGKDGIANMSLS
                     IYAYSTCRFWSKESVAVGDTIKLSNYEHIEFSFNSFSKDYFREQNSDIYKIFFKIASS
                     SNSVVVDKNTLTQKNFLENNTFFSITGIDESLYEKHIIFITLKVIVPSKK"
BASE COUNT          350 a          238 c          234 g          354 t
ORIGIN      
        1 ttcagttctt ccggctactg aaccgtcgat atgaaaaagc gagcatcata ctgacgtcaa
       61 acaaagggtt cgcagactgg ggagaaatgt tcggagagca tgtgctggca acagcgatcc
      121 tggaccggct gctacatcac tcaaccacgc tgaatatcaa aggagagagt taccggttca
      181 atattccaga cagactcgtg gttcatttga gcctccatgc tcgccctgaa ctccgggcac
      241 acaatacgtg atgtctcccg cccacttctg gttcgggcca ctggcgtaaa aatcctgctc
      301 cagcagattt tctgacacag gctggctgtg tgcgcggtaa cagaccggac tgaaattcca
      361 ggaagctttt gccctcagtc cgtgacggcg caggctttcc gtcacggttt ttactttaaa
      421 atggtaaccc tgggcacgca gttcatccat caggcgtttc gcgaagtatg tcgtggcctt
      481 tggaacgtct gctgatgctg ggtaagatta gtcagcaaat tccgcataca ggaaatcagc
      541 gctacttttt tgaggggaca ataaccttaa gagtgataaa gattatatgc ttttcataga
      601 gagactcatc tatacctgta atactgaaaa atgtattgtt ttctaggaag tttttttgag
      661 tgagcgtatt tttatccaca acaaccgaat tactcgaaga agcaatttta aaaaatattt
      721 tatatatatc actattttgt tcacgaaagt aatccttact aaatgaatta aagctaaact
      781 caatatgctc ataattagac aattttattg tatctcctac agccacactt tcttttgacc
      841 agaaacgaca tgtcgagtat gcgtatatag ataatgacat attggcaata ccgtcttttc
      901 cagcatactc agaaacagta tgtatatatc tattgataat attgttctgt aaatacgaat
      961 acgttggatt tctatatgct tcaatcaaat caaataattc atgtgaatat aaagaaggga
     1021 atgagttaga gtaaagtgga ttatgcaaga taaaacatcc attttttaat ggagtagggt
     1081 aaaaaaacaa cctgtcattt ccactgactg tttcctgtat ctttatcccc attggggtta
     1141 tatttattct attgtctgac aatttattat caatag
//
LOCUS       DAZFAB010000314         1165 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZFAB010000314 DAZFAB010000000
VERSION     DAZFAB010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.318"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>1165
                     /locus_tag="ACHJWA_005088"
     CDS             join(<1..262,262..>1165)
                     /locus_tag="ACHJWA_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611812.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1203 family transposase"
                     /protein_id="HFO4311484.1"
                     /translation="VRQRAIRMVLESQDEYDSQWAAICSIAPKIGCTPETLRVWVRQH
                     ERDTGGGDGGLTSAERQRLKELERENRELRRSNDILRQASAYFGEGGVRPPLEKMMPL
                     LDKLREQYGVGPVCSELHIAPSTYYHCQQQRHHPDKRSARAQHDDWLKREIQRVYDEN
                     HQVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRGKKVRTTISRKAVAAGDRV
                     NRQFVAERPDQLWVADFTYVSTWQGFVYVAFIIDVFAGYIVGWRVSSSMETTFVLDAL
                     EQALWARRPSGTIHHSDKGSQYVSLAYTERLKEAGLLASTGSTGDSYDNAMAESINGL
                     YKAEVIHRKSWKNRAEVELATLTWVDWYNNRRLLGRLGHTPPAEAEKAYYASIG"
     misc_feature    252..368
                     /locus_tag="ACHJWA_005088"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
BASE COUNT          279 a          275 c          360 g          251 t
ORIGIN      
        1 gtccgtcagc gggcgattcg tatggttctg gaaagtcagg atgaatatga ctcacagtgg
       61 gcggcaattt gttccattgc cccaaagatt ggctgtacgc cggagactct gcgtgtctgg
      121 gttcgccagc atgagcggga taccgggggc ggtgatggtg ggctcaccag cgctgaacgt
      181 cagcgtctga aagagctgga acgtgaaaat cgtgaactgc gccgcagtaa cgatatcctt
      241 cgccaggctt ccgcttattt tgcgaaggcg gagttcgacc gcctctggaa aaaatgatgc
      301 cactgctgga taagctgcgt gagcagtacg gggtcggacc ggtatgcagc gaactgcata
      361 ttgccccgtc aacgtattac cattgtcagc aacagcgaca tcatccggat aaacgcagtg
      421 cccgtgcgca gcacgacgac tggctgaaga gagagataca gcgcgtatac gatgaaaatc
      481 atcaggtgta cggtgtgcgt aaagtctggc gtcagttgtt acgggaagga atcagggtgg
      541 ccagatgtac agtggcacgt ctcatggcgg ttatgggact tgccggtgtt ctccggggta
      601 aaaaggtccg tacgaccatc agccggaaag ccgttgccgc aggcgaccgc gtaaaccgtc
      661 agttcgtggc agaacgacct gaccagctgt gggtggctga ttttacttac gtcagcacat
      721 ggcagggctt cgtctatgtg gcgtttatca ttgatgtgtt tgccggatac atcgtggggt
      781 ggcgggtctc atcgtctatg gaaacgacat tcgtgctgga tgcgctggag caggcgttgt
      841 gggcccgtcg tccgtctggc accatccatc acagcgataa aggctctcag tatgtgtcac
      901 tggcctatac ggagcgacta aaagaagccg gattactggc atcaacaggg agtacaggcg
      961 actcgtatga caacgcgatg gctgagagca tcaatggtct ttacaaagcg gaggtaatac
     1021 accgtaagag ctggaaaaac cgtgcagaag tggaactggc cacactaacg tgggtggact
     1081 ggtataacaa tcgacgattg ctgggaaggc tgggccatac tcctccggca gaagcagaaa
     1141 aagcttatta tgcttccatc ggaaa
//
LOCUS       DAZFAB010000315         1157 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZFAB010000315 DAZFAB010000000
VERSION     DAZFAB010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.319"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..661)
                     /gene="escT"
                     /locus_tag="ACHJWA_005089"
     CDS             complement(<1..661)
                     /gene="escT"
                     /locus_tag="ACHJWA_005089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscT"
                     /protein_id="HFO4311485.1"
                     /translation="MNEIMTVIVSSFYCILRPLGMFIILPIFSTGVLLSNFIRNSIMI
                     AFTLPIIVENYTFSEKLPSGIFQLTGIALKEISIGFFIGLSFTILFWAIDAAGQIIDT
                     LRGSTISSIFNPSISDSSSITGVILYQFISVIFVIHGGIQSILDKLYLSYEILPLQAD
                     IAFNRALIDFLFSLWDSFIKLMLSFSVPMIIGIFLCDMGFGFLNKTAPQLNVFTLSLP
                     VK"
     gene            complement(661..930)
                     /gene="escS"
                     /locus_tag="ACHJWA_005090"
     CDS             complement(661..930)
                     /gene="escS"
                     /locus_tag="ACHJWA_005090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscS"
                     /protein_id="HFO4311486.1"
                     /translation="MDTGYFVQLCVQTFWIIFILSLPTVIAASVIGIIISLVQAITQL
                     QDQTLPFLLKIIAVFATLALTYHWMGTTIINFSSIIFEMIPKVNG"
     gene            complement(930..>1157)
                     /gene="escR"
                     /locus_tag="ACHJWA_005091"
     CDS             complement(930..>1157)
                     /gene="escR"
                     /locus_tag="ACHJWA_005091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscR"
                     /protein_id="HFO4311487.1"
                     /translation="ILLPAFTMGQLEAAFKIGFLLYLPFIAIDLIISNILLALGMMMV
                     SPVTISIPFKILLFILVGGWQKLFEFLLVVN"
BASE COUNT          428 a          211 c          204 g          314 t
ORIGIN      
        1 tttttactgg cagtgataat gtgaatacgt ttagctgagg tgctgttttg ttaagaaacc
       61 caaaccccat atcacataag aatataccga taatcatggg aactgaaaat gataacatca
      121 gtttaataaa tgaatcccat agagaaaaca aaaaatctat taaagcacga ttgaatgcaa
      181 tatcggcttg taatggtaat atctcgtagg ataaatatag cttatctagg atgctttgta
      241 tcccaccatg aataacaaag atcacagaga taaattggta caagataacg ccagtgatag
      301 aagatgaatc acttatggac gggttaaaaa ttgaagatat tgttgagcct cttagagtat
      361 caataatctg tccagccgca tctattgccc aaaaaagaat agtaaatgat aacccaatga
      421 aaaaaccaat acttatttct tttagcgcta ttcctgtcaa ctgaaagatt ccagatggca
      481 atttttccga aaatgtatag ttctctacta tgatcggaag cgtaaacgca atcataattg
      541 agttgcgaat aaagttactg agtaaaacac ctgttgaaaa tattggtagg ataataaaca
      601 tccccagcgg cctgagtata caataaaatg atgatactat gaccgtcatt atctcattca
      661 ttagccgttc accttcggaa tcatttcaaa tattatcgac gagaagttga tgattgttgt
      721 tcccatccag tgataagtca gggcaagcgt agcaaacact gctattattt ttagcaaaaa
      781 aggcaatgtt tgatcctgta actgcgttat agcctggacc agactaataa taataccgat
      841 aacagaggcc gctatgactg taggcaatga gaggataaat attatccaga acgtttgcac
      901 acataattga acaaaatatc cagtatccat caattcacca ccaacagaaa ttcgaataat
      961 ttttgccagc ctccaacaag aatgaataat aagatcttaa aaggaatcga aattgttact
     1021 ggcgatacca tcatcatacc caaggccaat aagatattgg aaatgatcaa atctatcgca
     1081 ataaagggta aatagagcaa aaaacctatc ttgaatgcag cttcaagctg ccccatcgtg
     1141 aaagccggta acagtat
//
LOCUS       DAZFAB010000316         1152 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZFAB010000316 DAZFAB010000000
VERSION     DAZFAB010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.320"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..183
                     /locus_tag="ACHJWA_005092"
     CDS             <1..183
                     /locus_tag="ACHJWA_005092"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311488.1"
                     /translation="WRLAVVRCGWQQTITVTTRSPTKRSAIKKPESWNRRMPPLLTCR
                     CASVMLLRSMQNTRGN"
     gene            187..393
                     /locus_tag="ACHJWA_005093"
     CDS             187..393
                     /locus_tag="ACHJWA_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO4311489.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWAMMPP
                     SNSLRLLDETFSVSETESSATRQH"
     gene            458..682
                     /locus_tag="ACHJWA_005094"
     CDS             458..682
                     /locus_tag="ACHJWA_005094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311490.1"
                     /translation="MKKLLVTVKPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
     gene            1039..>1152
                     /locus_tag="ACHJWA_005095"
     CDS             1039..>1152
                     /locus_tag="ACHJWA_005095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311491.1"
                     /translation="MDFMLTVSGVVILSIAYTADKYGCHLLSRIGAYCSLML"
BASE COUNT          280 a          256 c          303 g          313 t
ORIGIN      
        1 tggcggttgg ctgtggtgcg ctgtggctgg caacaaacca ttaccgtgac aacgcgctca
       61 cctacaaagc gcagcgcgat aaaaaagcca gagagctgga acaggcgaat gccaccatta
      121 ctgacatgca ggtgcgccag cgtgatgttg ctgcgctcga tgcaaaatac tcgagggaat
      181 tagccgatgc gagagctgaa aatgaaactc tgcgtgctga tgttgccgct ggtcgtaagc
      241 gcctgcggat caacgccacc tgccccggta ccgtgcgtga agccaccggc acctccggcg
      301 tgggcaatga tgccgccgtc gaactctctc cggttgctgg acgaaacgtt ctcggtatca
      361 gagacggaat catcagcgac caggcagcat tgagaatgct tcaggaatat atccgcactc
      421 agtgtattaa ctagtatttt tgttattcgg agaatgcatg aagaaattat tggtaaccgt
      481 aaagcctttt cagggaacaa ttccgttccg tattttgcag cgtggtcgtg ttcttgttga
      541 aggttcgttc agtggtaaat gtacgcaatt acactccagg acctttcagg tgaatgccac
      601 gaatgaagag ctaaccgttg agtgtacgat gaatgccgct aaatgccgca tggtatccgc
      661 tgcattacag ccagtgtgtt gagcgacctt attattcatg cgcggtattg tcgccgtatt
      721 cctgcattaa cagagaccgc agcccgaccg ggagaatcct ctgcgcgagt gtgcggggat
      781 aatcaaaaac gatacacacc ggggtttacc gcgttaacgg agcgcggcgt tgtcccctca
      841 tggtcgctgg tccggtgcga tggtggaaga agccggatgt ttatcactat taattgataa
      901 cacagaaatg gattcattga ttttcagcac gtttttgtat tcgtattatt gaacatctgt
      961 ttattttact tttaacatat tgataataaa aagagctgta aatctttaga tgagtcgatt
     1021 ttgtccgggg aagttcaaat ggattttatg ctgacggttt ctggtgtggt tatcctgtcc
     1081 attgcttata ctgcagataa atatggctgc catttgttat cacgtattgg cgcttattgc
     1141 tcgttgatgc tg
//
LOCUS       DAZFAB010000317         1148 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZFAB010000317 DAZFAB010000000
VERSION     DAZFAB010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.321"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..441
                     /locus_tag="ACHJWA_005096"
     CDS             <1..441
                     /locus_tag="ACHJWA_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO4311492.1"
                     /translation="DEKEGNHTTAYRDGAGIWTICRGAILVDGKPVVPGMKLSKEKCD
                     QVNAIERDKALEWVERNIKVPLTEPQKAGIASFCPYNIGPGKCFPSTFYRRINAGDRR
                     GACEAIRWWIKDGGRDCRIRSNNCYGQVSRRDQESALACWGIDR"
     gene            597..779
                     /locus_tag="ACHJWA_005097"
     CDS             597..779
                     /locus_tag="ACHJWA_005097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310767.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311493.1"
                     /translation="MGKYKRILLRLTMKNGLELKAPVTDDVSRALAFAIKWVAVGIAV
                     SPMLYGLAKLVIALKS"
     gene            792..923
                     /locus_tag="ACHJWA_005098"
     CDS             792..923
                     /locus_tag="ACHJWA_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311494.1"
                     /translation="MSDKLITLAKILCVIVGISFSLMLVAICFSTAWRVLTLSWLVG"
     gene            931..>1148
                     /locus_tag="ACHJWA_005099"
     CDS             931..>1148
                     /locus_tag="ACHJWA_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
                     /protein_id="HFO4311495.1"
                     /translation="MNRVLCVVIIVLLVACGVLSLGLNHYRDNAITYKAQRDKKASEL
                     KLANVTITDMQVRQRDVAALDARYSRELA"
BASE COUNT          288 a          237 c          341 g          282 t
ORIGIN      
        1 gacgaaaaag aaggtaacca caccacggca taccgtgatg gtgcgggtat ctggaccatc
       61 tgccgcggtg ccatcctggt ggatggtaaa cctgtcgttc cgggcatgaa gttgtcgaag
      121 gaaaaatgcg accaggttaa cgccattgaa cgtgataagg cgctggagtg ggtggagcgc
      181 aatattaaag taccgctgac cgaaccccag aaagcgggga tcgcgtcatt ctgtccgtac
      241 aacattggtc ccggtaagtg tttcccgtcg acgttttaca gacgaattaa tgcaggtgat
      301 cgcaggggag cgtgtgaggc gattcgctgg tggattaagg acggtggcag agactgccgt
      361 attcgctcaa acaactgcta cggtcaggtt tcacggcgtg accaggagag cgcgctggcg
      421 tgctggggaa ttgacagata agcagaatat tttgctgaaa aatgcggttt gctcacactg
      481 gcggataaca cgaaatcctg cgaactggca aaaactaagt gaataaaagt aaaaccccgt
      541 ttgttggccg caaacggggt tttgtgtttc tgaccttgag taaggcaagg gagaacatgg
      601 gaaagtataa acgaattctg ttgagattga ctatgaaaaa cggccttgaa ctgaaagcgc
      661 ctgtaactga tgacgtcagc agagcgctgg cttttgctat taagtgggtg gcggtcggta
      721 ttgctgtgtc tccgatgctg tatgggctgg caaaactggt cattgcgttg aaatcgtgaa
      781 gaggattaag catgtcagac aagctcataa cgctggcgaa gatcctctgt gtaatcgtcg
      841 gcatttcatt ttcactaatg ctggttgcta tttgcttttc cactgcctgg cgcgtcttaa
      901 ctttgtcatg gctggtgggg tgagggggat atgaaccgtg ttctgtgtgt ggtgattatt
      961 gtcctgctgg tagcctgtgg tgtgcttagt ctggggctga atcattaccg cgataacgcc
     1021 attacctaca aagcgcaacg cgataaaaaa gccagtgagc tgaagctggc gaacgtgaca
     1081 attactgata tgcaggtacg ccagcgtgat gtcgctgcac ttgatgccag atactcgagg
     1141 gaattagc
//
LOCUS       DAZFAB010000318         1146 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZFAB010000318 DAZFAB010000000
VERSION     DAZFAB010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.322"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..81
                     /locus_tag="ACHJWA_005100"
     CDS             <1..81
                     /locus_tag="ACHJWA_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311496.1"
                     /translation="VRAQPVSGGRLGVKIYPIMLEGQVKK"
     gene            78..899
                     /locus_tag="ACHJWA_005101"
     CDS             78..899
                     /locus_tag="ACHJWA_005101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO4311497.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDTLSITDVMAAQGMTQNW
                     AEMGFSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDFRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICNVTDAISLDTWKKTVKRFYDTLVVQFDIEEAWAEQQLKKVTR"
BASE COUNT          312 a          219 c          325 g          290 t
ORIGIN      
        1 gttcgtgctc agccagtatc tggtggacgt ctgggggtga agatttaccc cataatgctt
       61 gaagggcagg tcaaaaaatg aaactggaag atttaccgaa atactactcc ccaaaatccc
      121 ccggcctgac tgatgcatcg gcctcaacgt cgaaagatac gctgagtatc actgatgtga
      181 tggccgcgca gggcatgaca cagaattggg ctgagatggg gttttctgcg ttccttggga
      241 aaatgggcat tagtatgaat gacagagagc gggcaacaga attgctgaca gaatatgcac
      301 tcagtcggtg tgatcgcgtg gcggcgttaa gaaaactccc ggcagaaata aaaccggcag
      361 tgatgcgtat tatggcttcg tatgcgtttg aagattatgc ccgtagcgcg gcgagcaaaa
      421 aacagtgccc ctgttgtcac ggaaaaaaat ttattgaaag cgaggttttt acaaacaaga
      481 tccagtatcc ggatggtaag ccgccagtgt gggcaaagtg cacaaaaggc gtgtatccgt
      541 cttactggga ggaatggaaa aaagtcaggg aggtggtaaa agttgcctgt ccggagtgtg
      601 gagggaaggg ggaggtttcc accgcctgta aagattttcg tgggcgcggt gttgccattc
      661 atcgtgaaga gtcggtaaaa cgtggtatgc ctgttatcag agactgccag cgttgtggtg
      721 gtcgtggcta tgaaagatta ccttcaacgg aggcatttaa tgccatatgt aatgtaaccg
      781 atgccatatc tcttgataca tggaaaaaaa cagttaaacg tttttacgat acgctggtgg
      841 tgcagtttga tattgaagaa gcatgggcag aacaacaact gaaaaaggtg accagatagc
      901 tttgttgatt tttcccgaat ctgtggtaaa tttgccctaa cgatgggcgt tttatgcctg
      961 acgttagaag attttttaca cccgtcgcca ggcgggtttt tttatgactg aaatcacgcc
     1021 agtacagtaa acgcgctggt ggttgtgaat accggtcttt ctgcttgctg gctttttcga
     1081 caagagttat tggtgtgtca cgttaaccgg aaaaaggaaa gtttgagaaa cgcgatctgg
     1141 cacagg
//
LOCUS       DAZFAB010000319         1137 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZFAB010000319 DAZFAB010000000
VERSION     DAZFAB010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.323"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            110..349
                     /locus_tag="ACHJWA_005102"
     CDS             110..349
                     /locus_tag="ACHJWA_005102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
                     /protein_id="HFO4311498.1"
                     /translation="MADWNGYIMDISKQFDQGVDDLNQQVEKALEDLATNPSDPKFLA
                     EYQSALAEYTLYRNAQSNVVKAYKDLDSAIIQNFR"
     gene            369..701
                     /gene="sctI"
                     /locus_tag="ACHJWA_005103"
     CDS             369..701
                     /gene="sctI"
                     /locus_tag="ACHJWA_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HFO4311499.1"
                     /translation="MSVSNMPPIDRAEQSTAHEIQQAKVIDLNDRVLNLDNPDDKMIS
                     AFANYAVQTENWQQNALQALTSDKKGLTPEKLLVLQDHVLNYNVEVSLVGTLARKIVA
                     AVETLTRS"
     gene            698..>1137
                     /gene="sctJ"
                     /locus_tag="ACHJWA_005104"
     CDS             698..>1137
                     /gene="sctJ"
                     /locus_tag="ACHJWA_005104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner membrane ring
                     lipoprotein SctJ"
                     /protein_id="HFO4311500.1"
                     /translation="MKYISLLLFILLLCGCKQQELLNHLDQQQANDVLAVLQRHNINA
                     EKKDQGKTGFSIYVEPTDFASAVDWLKIYNLPGKPDIQISQMFPADALVSSPRAEKAR
                     LYSAIEQRLEQSLKIMDGIVSSRVHVSYDVDTGDSGKTALPIHIS"
BASE COUNT          371 a          203 c          236 g          327 t
ORIGIN      
        1 aatttgaaaa atcaggacac aaataatagt tcatttagct acggagataa ccgattcgag
       61 aagtctcaag gtagcaagtg gacatttcag gaataattga ggaggactta tggcagattg
      121 gaatggttat attatggaca tcagcaaaca atttgatcag ggcgttgatg atctgaacca
      181 gcaagttgaa aaagcgttgg aggatttagc aaccaatcct tccgacccga aattccttgc
      241 cgaatatcaa agtgcattag ctgagtatac attatatcga aatgcgcaat ctaacgttgt
      301 aaaagcgtat aaagatcttg attcggcaat catacaaaac ttccgctaaa ctggataagg
      361 attttattat gtcagtaagc aatatgccac ctattgatag agctgagcaa tcaactgctc
      421 atgaaataca gcaagcgaaa gtgattgatt taaatgaccg ggttctcaat cttgacaacc
      481 cggatgataa aatgatctcc gcttttgcta actatgctgt acaaactgaa aattggcagc
      541 aaaatgcgtt acaagcatta acgtctgata aaaaaggatt aactcctgaa aaattattag
      601 tcttgcagga tcatgtgctt aattataatg tcgaggtttc attggtggga acattagcaa
      661 gaaaaattgt cgctgcagtt gagacgttga cccgctcatg aaatatatat cattattatt
      721 gtttatcctg ctgttgtgcg gctgcaagca gcaggaatta ctgaaccatc ttgatcaaca
      781 gcaagctaat gatgtccttg ctgttttaca aagacataat attaatgctg aaaagaagga
      841 tcaaggcaaa actggattct caatctatgt tgaaccaacg gattttgctt ctgcggtgga
      901 ttggctaaaa atctacaatc tcccgggtaa gcctgatatt caaatatcgc agatgttccc
      961 tgcagatgca ctcgtttcat ctccacgagc ggaaaaagca aggctatatt ctgctattga
     1021 gcagcggtta gagcaatctc tcaaaattat ggatggtatt gtttcttcga gagtacatgt
     1081 tagttacgat gttgatactg gcgatagtgg taaaactgct ttaccaattc atatttc
//
LOCUS       DAZFAB010000320         1104 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZFAB010000320 DAZFAB010000000
VERSION     DAZFAB010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.324"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..121)
                     /locus_tag="ACHJWA_005105"
     CDS             complement(<1..121)
                     /locus_tag="ACHJWA_005105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO4311501.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERC"
     gene            complement(346..900)
                     /locus_tag="ACHJWA_005106"
     CDS             complement(346..900)
                     /locus_tag="ACHJWA_005106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFO4311502.1"
                     /translation="MIYPEITGKSGEHLRLKTLESVWIQGKLRMWGRWSYIGDGKTGN
                     MFNQLLTSKKLTKTAINEALRRMKKAGLDKPELEAFLRDMINGNQKSWLAHCTDSEAL
                     IIDRVIGEVLAGYPGLLNVLSQRYVGRGMTKRKMAELLNDAHPEWSLRTCERRIEHWL
                     KVAEFILYKPMVMAFGIEKKVIAF"
     gene            complement(963..>1104)
                     /locus_tag="ACHJWA_005107"
     CDS             complement(963..>1104)
                     /locus_tag="ACHJWA_005107"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO4311503.1"
                     /translation="IKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIV
                     RG"
BASE COUNT          268 a          308 c          207 g          321 t
ORIGIN      
        1 ggcagcgttc gcgtgaaagg taagtattct gcgcaatctc cccgactgtc gccggttcgg
       61 taacgcttaa ttcattaaac accactctgg cggtttctgt catatcctgc tgttttagca
      121 tgtctttttc ccttttccgg ttaacgtgac ataccaataa ctcttgtcga aaaagccagc
      181 aagttgaaag accggtatta gcaaccacca gcgcgtttaa cgccccgtgc cgtttttcag
      241 tcataaaaaa acccgcaaaa agcgggctct ttcaaatgtc catgtctgct attcgcctcg
      301 cggtacagct ttgcgaagct taccggaatt gaagcagttt ttacgtcaaa aagcaataac
      361 ttttttctct ataccaaaag ccataaccat tggtttgtac aaaataaatt ctgccacctt
      421 tagccaatgc tcaatgcgtc tttcacaggt tcttaaactc cattccggat gtgcatcatt
      481 cagcaattca gccattttgc gcttagtcat cccccgcccc acataacgct gactcagaac
      541 attgagcagc ccgggataac ctgccagtac ttcaccaata accctatcga ttattaacgc
      601 ctctgaatcg gtacaatgtg ccagccagct tttttgattg ccgttgatca tatcccgcaa
      661 aaaagcctca agttcaggtt tgtccagacc cgcttttttc atcctccgga gcgcctcgtt
      721 aattgccgtt tttgtcagct ttttagaggt cagtaattgg ttgaacatat ttcccgtctt
      781 accgtcgcca atatacgacc aacgccccca catacgcagt ttcccctgga tccagacact
      841 ttccagcgtt ttcaggcgta aatgctcacc gcttttgcct gtaatttccg ggtatatcat
      901 atttatgctc actcactttc aattttgtaa atcttcacgc ccagccgccc accagaaacg
      961 agctagccgc gcacaatatt gatttcatca aactgctcgt cgtctatgag aagtccggca
     1021 tgcgtcagcg catccagtgg tgctttcagg atattgtcca ggtcacgacg gcgcttatcc
     1081 ggtggctctg caataatctt tatc
//
LOCUS       DAZFAB010000321         1104 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZFAB010000321 DAZFAB010000000
VERSION     DAZFAB010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.325"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..168
                     /locus_tag="ACHJWA_005108"
     CDS             <1..168
                     /locus_tag="ACHJWA_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO4311504.1"
                     /translation="YAKAGMIFPLPKKVGRRWRVDPQARFVGMVNKPEVIATDHPALK
                     RILEDGAPAKI"
     gene            146..>1104
                     /locus_tag="ACHJWA_005109"
     CDS             146..>1104
                     /locus_tag="ACHJWA_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFO4311505.1"
                     /translation="MARPRKYKTDVPGLSPYFDKRNNKVYWRYRHPITGKNHGLGSID
                     QKLAETIAAEANSRLARQQMEQMLSLQEKIISDTGGSSTVTIFLNNYRKIQQERYENG
                     EIKLNTLKQKAAPLRVFDERFGTRPLDAITVKDVVSVLEEYKARGHNRMGQIFRKVLI
                     DVFREAQQTGDVPPGFNPAESAKKPQVRISRQRLTFDEWMMIYNAAEKDGYFLQRGML
                     LALMTGQRLSDICKMQFSDIRDGYLHVEQQKTGTRIAIPLALRCDKLNLTLDDVVSSC
                     RDCVLSPWLLHHHHAKGTAKRGGMVKPATLTVAFKKARDSVDY"
BASE COUNT          337 a          246 c          274 g          247 t
ORIGIN      
        1 tacgctaaag ccggaatgat atttcctctc cccaaaaaag ttggaagacg ctggcgagtg
       61 gatccgcaag ctcgctttgt cggaatggta aacaagccgg aggtgatcgc cacagatcac
      121 cctgctttga agaggatact ggaagatggc gcgcccgcga aaatataaaa ccgatgttcc
      181 gggattatct ccgtattttg acaaaagaaa taacaaagtt tactggcgtt acaggcatcc
      241 cataacaggc aaaaatcacg gtctcggcag tattgaccag aaactggcag aaactattgc
      301 agcagaagcg aacagccgtc ttgcccggca gcaaatggaa caaatgctca gtctgcagga
      361 gaaaattatt agtgataccg gcggttcatc aaccgttacc atttttctga ataattacag
      421 aaaaattcaa caggaaagat atgaaaacgg cgagatcaaa ctcaacacgc tgaaacagaa
      481 agcggcccct ctcagggtat ttgatgaacg ttttggcacc agaccgttag atgccataac
      541 cgtaaaagat gtggtatcag tactggaaga gtacaaggcc agaggacata acagaatggg
      601 acaaattttc aggaaagtac tgatcgatgt tttccgggaa gctcagcaaa cgggcgatgt
      661 cccgccaggc tttaaccctg cagaatcggc aaaaaaaccg caggtgcgga tatcaagaca
      721 gcgactgact tttgatgagt ggatgatgat ttataacgca gcggaaaagg atggttactt
      781 tttacagcgc ggtatgctgc tggcactgat gacaggccag cgcctttcag atatttgcaa
      841 aatgcaattt tcggatatcc gggatggtta tcttcatgtc gaacagcaaa aaacaggaac
      901 ccggattgcc atccctctgg ctctgcgttg cgataaatta aatctcaccc tggatgatgt
      961 ggtgtcatcc tgccgcgatt gcgttcttag tccgtggcta ttgcaccacc atcacgcgaa
     1021 agggacagct aagcgcggcg ggatggttaa gccagcaaca ttaaccgttg catttaaaaa
     1081 agcccgggat tctgtggatt acaa
//
LOCUS       DAZFAB010000322         1100 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZFAB010000322 DAZFAB010000000
VERSION     DAZFAB010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.326"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..135)
                     /locus_tag="ACHJWA_005110"
     CDS             complement(<1..135)
                     /locus_tag="ACHJWA_005110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO4311506.1"
                     /translation="MKISVITVTYNNAEGLEKTLSSLSILKIKPFEIIIVDGGSTDGT
                     N"
     gene            complement(104..>1100)
                     /gene="wzy"
                     /locus_tag="ACHJWA_005111"
     CDS             complement(104..>1100)
                     /gene="wzy"
                     /locus_tag="ACHJWA_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O157 family O-antigen polymerase"
                     /protein_id="HFO4311507.1"
                     /translation="KKFIVNWELKLILVFLFVTIEIAAVVLFLKEGIPIFDDDPGGAK
                     LRIAEGNGLYIRYIKYFGNIVVFALIILYDEHKFKQRTIIFVYFTTIALFGYRSELVL
                     LILQYILITNILSKDNRNPKIKRIIGYFLLVGVVCSLFYLSLGQDGEQNDSYNNMLRI
                     INRLTIEQVESVPYVVSESIKNDFFPTPELEKELKAIINRIQGIKHQDLFYGERLHKQ
                     VFGDMGANFLSVTTYGAELLVFFGFLCVFIIPLGIYIPFYLLKRMKKTHSSINCAFYS
                     YIIMILLQYLVAGNASAFFFGPFLSVLIMCTPLILLHDTLKRLSRNENISYNCDL"
BASE COUNT          400 a          200 c          137 g          363 t
ORIGIN      
        1 attcgttcca tctgtagagc cgccatcaac tataataatc tcaaaaggtt ttatttttaa
       61 aattgataaa ctacttaaag ttttttctaa cccttcagca ttattataag tcacagttat
      121 aactgatatt ttcatttcgt gataatctct ttaacgtatc atgcaataag atcagaggag
      181 tacacattat caatacggag agaaaaggac caaaaaagaa ggccgatgca ttcccagcca
      241 ctaagtattg caataaaatc ataatgatat atgaatagaa tgcgcaattt atcgagctat
      301 gggttttttt cattctcttt aaaagataaa aaggtatata tatccctaaa gggataatga
      361 atacacagag aaaaccaaaa aaaactaaca gttctgctcc atacgtagta actgataaaa
      421 aatttgctcc catgtctcca aatacttgtt tatgtaaccg ttctccataa aataagtctt
      481 gatgctttat tccctgtatt ctatttatta ttgcttttaa ttccttttct aactctggtg
      541 tcggaaagaa atcgttctta atagattcag aaacaacata tggaacactt tcaacttgct
      601 ctattgttaa cctattaatt atccttaaca tattattata tgagtcattt tgttctccgt
      661 cttgtcctaa acttagataa aacaacgagc atacaacccc taccaataaa aaatacccta
      721 ttattctttt tattttagga ttacggttat cctttgacag gatattggta atcaatatat
      781 attgaagaat gagcaacacc aattcagaac gataaccaaa taaagcaatc gttgtaaaat
      841 atacaaatat gatggtcctc tgtttgaatt tatgctcatc ataaagaata attaatgcaa
      901 acacaactat attaccaaaa tacttaatat atctaatgta aagtccatta ccttcagcta
      961 ttctaagttt agccccccct ggatcatcat caaatatagg aataccttct ttaagaaata
     1021 aaactacagc agcaatttct atagtcacaa aaaggaaaac gagtattaac tttagctccc
     1081 agttcacgat aaattttttc
//
LOCUS       DAZFAB010000323         1096 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZFAB010000323 DAZFAB010000000
VERSION     DAZFAB010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.327"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..903)
                     /locus_tag="ACHJWA_005112"
     CDS             complement(<1..903)
                     /locus_tag="ACHJWA_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFO4311508.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTRTPAPVGIFGPGWKAPSDI
                     RLQLRDDALVLNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKAAQPDGHTLARLWG
                     ALPPDIRLSPHLYLATNSAQGPWWILGWSERVPGAEDVLPAPLPPYRVLTGMADRFGR
                     TLTYRREAAGDLAGEITGVTDGAGREFRLVLTTQAQRAEEARKQHTASLSSPDTPRPL
                     SDSAFPDTLPGTEYGPDRGIRLSAV"
BASE COUNT          228 a          355 c          298 g          215 t
ORIGIN      
        1 caccgccgaa aggcggatac ctctgtcggg accgtattcg gtaccgggca gtgtgtcggg
       61 gaacgctgag tctgaaagag ggcggggggt gtcaggggaa gataaagaag cggtgtgctg
      121 tttacgggcc tcttccgccc gctgcgcctg cgtggtcagc accagacgga actcccgccc
      181 ggcaccgtcc gtcacgccgg tgatttcccc ggccaggtca ccggcggcct cacgcctgta
      241 cgtcagcgtc cgcccgaagc ggtccgccat cccggtaagc acccggtacg gcggcagcgg
      301 cgctggcagt acgtcctcag cacccggcac ccgctctgac caccccagta tccaccacgg
      361 cccctgtgcg ctgttggtcg ccaggtaaag atgcgggctt aaccggatat ccggcggcag
      421 cgccccccac agccgcgcca gcgtgtggcc gtccggctgc gctgccttac caccgcgcac
      481 cagccacatt gactcgctgc ggctgtacac cgcctccccc ggcagcagcg gctcaaagtg
      541 aatgctccgc ccgccgttgt cattgagtac cagtgcatca tcgcgtagct gtaagcggat
      601 atcagaaggc gctttccagc cggggccaaa aatccccacc ggcgcaggcg tccgggtccg
      661 gtagctgctg taggtgcggg agagaatgaa cggcagcggg ccgggcagcg caaggtccgt
      721 ctcgccgggc agcaccttcg cccccagcag cggatttacc gggttgcccg aagtcatccc
      781 gcccgggcac accgagcacg ccacgccagt tggcgcgcca attcttacac ctgccgaacc
      841 ctggacaatg ggaccgccat actgagtcat atctccctga cgcgccgctg gttttccgct
      901 catataaagt gcccttttag ttattgttca tatccctgtg ttagcaacaa aaaaaataaa
      961 atgacacaca agaccagcta atgtatttat atatttttca cataaactta tttgttctca
     1021 atcaacaaca actgttatta caggtcactt tcaatatttt attaaactaa aatttttagc
     1081 acagatgaat tcatca
//
LOCUS       DAZFAB010000324         1090 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZFAB010000324 DAZFAB010000000
VERSION     DAZFAB010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.328"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(112..801)
                     /locus_tag="ACHJWA_005113"
     CDS             complement(112..801)
                     /locus_tag="ACHJWA_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFO4311509.1"
                     /translation="MNTQYLQYVREQLMAATADLNGATKGQLEAWQEHAQFDTGTYKR
                     KKPRILDVVTGKMITLDNTPTSGKQSYAKGSSIALVSPVEFSTSSWRRAVLSLDEHQK
                     AWLLWCYSESVRWGHQVTITQWAWSEFKDLLSNRKIAGKTLDRLKTLIWLAAQDVKSE
                     LAGREAYEYQTLASLVGVTTKNWSETFTERWVAMKHIFLQLDSDALLLVTRTRSKQKA
                     AFLQQNIAKLD"
     gene            complement(816..938)
                     /locus_tag="ACHJWA_005114"
     CDS             complement(816..938)
                     /locus_tag="ACHJWA_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000971054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFO4311510.1"
                     /translation="MMSESYISELLRCRWGLLCLCRFPDSVLNDYRMLKNYAKI"
BASE COUNT          303 a          267 c          198 g          322 t
ORIGIN      
        1 gcagccagca tgtttaccgt acaagtacaa ctcagggcat aaaaaaaccc actcggcagc
       61 gggtttatac attttttaca acataccaaa tttgcatgaa gtatatggct tttaatccag
      121 ttttgcaata ttttgctgta aaaatgctgc cttttgtttt gaacgtgttc tcgtcacaag
      181 caataaagca tcactatcaa gctgtagaaa aatgtgcttc attgcaaccc agcgttcagt
      241 aaatgtctcg gaccagtttt ttgttgtcac tcccaccaat gatgccagtg tctggtattc
      301 ataggcctca cgccctgcaa gttcgctctt cacatcctgt gcagccagcc agattaacgt
      361 cttcaggcga tccagtgtct tacctgcaat ttttctgtta cttaacaaat ctttaaactc
      421 gctccatgcc cattgcgtta tggtgacctg atgcccccat cgaacgcttt cgctgtaaca
      481 ccaaagcaac catgctttct gatgttcatc gagagacaaa accgcgcggc gccatgaaga
      541 ggttgagaat tcaaccgggc tgaccaaagc aatggatgaa ccttttgcgt acgactgctt
      601 accggaagtc ggcgtattat ccagcgtaat catcttgcca gttaccacat ccagaatgcg
      661 cggcttcttt cgtttgtatg taccagtatc aaattgtgca tgctcctgcc aggcttcgag
      721 ctggcctttc gttgctccgt tcaagtcagc agtagctgcc ataagttgct cacgaacata
      781 ctgtaaatat tgggtattca tgcagtaaat cctttctata ttttggcata attcttcaac
      841 attcggtaat cgttcaaaac cgaatcgggg aaacgacata agcacaggag cccccagcga
      901 cagcgaagga gttctgatat ataagactca gacatcattc attccccggt tctccaatat
      961 ctgtttcact catcatccat aactacctgt aattgccccc cctttttttg taacagttct
     1021 tatattgcta tatagaatag ccattactaa tgcatttaaa tttaataaaa taaaattata
     1081 aaaaacataa
//
LOCUS       DAZFAB010000325         1080 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZFAB010000325 DAZFAB010000000
VERSION     DAZFAB010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.329"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            120..1079
                     /locus_tag="ACHJWA_005115"
     CDS             120..1079
                     /locus_tag="ACHJWA_005115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A deacylase LpxR family protein"
                     /protein_id="HFO4311511.1"
                     /translation="MKKSVIAGVFIALSFTTCSAIANSLALSLANDDAGKFQPILNDI
                     YGNKHENRDDYSQGLFLGYSHDISDSSQLSLHIAQDIYSPSGSNKRHNTAVTGDRAFS
                     AYTHTGIEWNSLANDWIRYRLGTDIGVVGPDAGGQKVQNKAHEIIGAEKYHAWDDQIE
                     NRYGYTVKGMLSMTPSMDILGANVGLYPEVSAVTGNLFQYVAYGATIAIGNDKTFNSD
                     NGFGLLAPRGLMHMSDTSGFKYKIFAGMERRDVNRNYTLEGKTIQTKQTTVSLNKTVD
                     EYQVGATIGYAPVAFTLAFNKVTSEFKTGDDYSFINGAITFFF"
BASE COUNT          367 a          208 c          208 g          297 t
ORIGIN      
        1 aacgaataaa tcaattgtct cacgcaatta taaaacatca tattgattac gcaccttgca
       61 ttacaaactc atgtatgtaa aatacgcgca ccattcaaaa aaaaggaaga caataacata
      121 tgaaaaaaag tgtcatcgct ggcgtcttta ttgctctgtc atttaccacg tgttcagcta
      181 tcgcgaacag ccttgcatta tcattagcaa atgatgatgc agggaagttt caaccaatac
      241 ttaatgatat ttatggcaat aaacatgaaa acagagatga ttactcacaa ggcttatttc
      301 tgggatatag ccacgatatc tcagactcga gccaattatc tctccatatt gcgcaagata
      361 tttactctcc atcaggcagt aataaaagac acaacacagc tgtaactgga gacagagctt
      421 ttagtgcata cactcacact ggtattgaat ggaactccct tgcgaatgac tggattcgct
      481 atcgattagg tactgacata ggtgttgttg gccccgacgc aggcggtcag aaagtacaaa
      541 ataaagctca tgagattatt ggggcagaaa aatatcatgc atgggatgat caaatagaga
      601 atcgctacgg ttatactgta aaagggatgc tatccatgac accaagtatg gatattttag
      661 gtgctaatgt tggattatac cctgaagttt ctgctgttac tggaaactta tttcaatatg
      721 tagcatatgg cgcaaccatt gccattggta atgataaaac cttcaattcg gataatggct
      781 ttggtctgct ggctccccgt ggtttaatgc atatgtccga tacaagcgga ttcaaataca
      841 agatttttgc aggtatggaa agacgagatg tcaatcgcaa ctatactctc gaaggaaaaa
      901 caatacagac gaaacaaaca acagtatcgc taaacaaaac tgttgatgaa tatcaagttg
      961 gcgcaacaat tgggtatgca cctgtagcct tcacactagc atttaataaa gtaacatcag
     1021 aattcaagac aggggatgac tattcattta taaatggagc aatcaccttc tttttttaac
//
LOCUS       DAZFAB010000326         1072 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZFAB010000326 DAZFAB010000000
VERSION     DAZFAB010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.330"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..203)
                     /locus_tag="ACHJWA_005116"
     CDS             complement(<1..203)
                     /locus_tag="ACHJWA_005116"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311512.1"
                     /translation="MCRKYAHVVDALQGNFPYATDPAGNRLPDPELHPDSTLTAWPDN
                     RIAEDAHYVYRYDEYGRLAEKTDR"
     gene            complement(361..1065)
                     /locus_tag="ACHJWA_005117"
                     /pseudo
     CDS             complement(361..1065)
                     /locus_tag="ACHJWA_005117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625207.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
BASE COUNT          321 a          259 c          173 g          319 t
ORIGIN      
        1 cggtccgtct tctccgccag cctgccgtat tcatcgtagc ggtagacata gtgcgcatct
       61 tccgcgatgc ggttatccgg ccatgccgtg agggtgctgt ccgggtgcag ttccgggtcc
      121 ggcagccggt ttcctgccgg gtctgttgca tacgggaaat tcccttgcag ggcgtccaca
      181 acatgtgcat atttcctgca catgctgtgg ttttgtcata caaggagcaa caatactagt
      241 taatgattca taaaaaattt taacggggtg atttttcatg cgattatcgt cctaattaac
      301 gccacctcac ttacagatta tttcattcgt tacaatcttt tgattgcatt acacccaggc
      361 ttattcagaa acatgagaaa tataaccata taaataaaaa ataattctct tatcccccat
      421 caaaatagcc aaatccatag gggagtatcc catctcactc tccaaataaa tatcagcacc
      481 taatttcaac aaaagcttca tcatatcaaa atcccctatc cctatcgaat aatgcaaagg
      541 agtgaagtca aagccattat ttgccttagc atttatatcg gcaccatgtt gaataaaaca
      601 tctgacatct aattgtcttc ttttcctaac agctatatga agaaccccct cgccaagttc
      661 tgtccttgca tctgtatcta taatctctcc accaaaatat cgctcaccgt agatattaat
      721 aagtacagaa aaatcgggta tgggattatg atctctataa tgtgatagcc attcaactat
      781 tttataattc tggtgtacct cagccaattc taatgccgtt ttcccaaatt catcctttat
      841 agtaagatcg gctcctgatt ctactaaaag cttaacaata tcaaaatacc cattacttac
      901 ggcctcatta agtgcggtac gccccaaatc cccacgagca tccagatcca cacccaattt
      961 aattaaatta cttacaacct cagtctcacc taaaaatgca gcctgagtta taggataata
     1021 atttaatttt gacttaaggt ggtaattttt cggcttaaaa aaaaatcagg ca
//
LOCUS       DAZFAB010000327         1068 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZFAB010000327 DAZFAB010000000
VERSION     DAZFAB010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.331"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..553
                     /gene="nleB"
                     /locus_tag="ACHJWA_005118"
     CDS             <1..553
                     /gene="nleB"
                     /locus_tag="ACHJWA_005118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB"
                     /protein_id="HFO4311513.1"
                     /translation="ASERDNYLLGLLREELKNIPEGKDSLIESYAEKREHTWFDFFRN
                     LAMLKAGSLFTETGKTGCHNISPCSGCIYLDADMIITDKLGVLYAPDGIAVHVDCNDE
                     IKSLENGAIVVNRSNHPALLAGLDIMKSKVDAHPYYDGLGKGIKRHFNYSSLHDYNAF
                     CDFIEFKHENIIPNTSMYTCSSW"
     gene            602..>1068
                     /gene="nleE"
                     /locus_tag="ACHJWA_005119"
     CDS             602..>1068
                     /gene="nleE"
                     /locus_tag="ACHJWA_005119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase NleE"
                     /protein_id="HFO4311514.1"
                     /translation="MINPVTNTQGVSPINTKYAEHVVKNIYPEIKHDYFNESPNIYDK
                     KYISGITRGVAELKQEEFVNEKARRFSYMKTMYSVCPEAFEPISRNEASTPEGSWLTV
                     ISGKRPMGQFSVDSLYNPDLHALCELPDICCKIFPKENNDFLYIVVVYRNDSPL"
BASE COUNT          345 a          164 c          225 g          334 t
ORIGIN      
        1 tgccagtgag agggataatt atctgttagg cttattaaga gaagagttaa aaaatatccc
       61 agaaggtaag gactctttga ttgagtcata tgcagaaaaa agagaacata cttggtttga
      121 ttttttcagg aatttggcca tgttgaaggc tggaagtttg tttacagaga cgggaaaaac
      181 tggatgccat aacatatcgc cctgtagcgg atgtatatat cttgatgccg acatgattat
      241 taccgataaa ttaggagtcc tgtatgctcc tgatggtatc gctgtgcatg tagattgtaa
      301 tgatgagata aaaagtcttg aaaatggtgc gatagttgtc aatcgtagta atcatccagc
      361 attacttgca ggcctcgata ttatgaagag taaagttgac gctcatccat attatgatgg
      421 tctaggaaag ggtatcaagc ggcattttaa ctattcatcg ttacacgatt ataatgcttt
      481 ttgtgatttt attgaattta agcatgaaaa tattataccg aataccagta tgtatacctg
      541 cagttcatgg taagtcttcc ggttgtggtt gaaaaatatc gttctttagg tatagattca
      601 gatgattaat cctgttacta atactcaggg cgtgtcccct ataaatacta aatatgctga
      661 acatgtggtg aaaaatattt acccggaaat taaacatgat tactttaatg aatcacccaa
      721 tatatatgat aagaagtata tatccggtat aaccagagga gtagctgaac taaaacagga
      781 agaatttgtt aacgagaaag ccagacggtt ttcttatatg aagactatgt attctgtatg
      841 tccagaagcg tttgaaccta tttccagaaa tgaagccagt acaccggaag gaagctggct
      901 aacagttata tccggaaaac gcccaatggg gcagttttct gtagatagtt tatacaatcc
      961 tgatttacat gcattatgtg agcttccgga catttgttgt aagatcttcc ctaaagaaaa
     1021 taatgatttt ttatacatag ttgttgtgta cagaaatgac agccctct
//
LOCUS       DAZFAB010000328         1060 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZFAB010000328 DAZFAB010000000
VERSION     DAZFAB010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.333"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..225
                     /locus_tag="ACHJWA_005120"
     CDS             <1..225
                     /locus_tag="ACHJWA_005120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001509878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311515.1"
                     /translation="NDNDEKVKIENDIKKIDKKIEEIDNAIRDLSSETERFKIKYQQE
                     NTLNDVVQNIRTWLKENQNMVKAIKKIDTE"
     gene            complement(694..>1060)
                     /locus_tag="ACHJWA_005121"
     CDS             complement(694..>1060)
                     /locus_tag="ACHJWA_005121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311516.1"
                     /translation="FNNSEMNFISCVGTNFSGSTFNNVKTTTAQLIKTPTKWTNNTLK
                     YWFSSCNKRNIIFTFNTISDRNMKLKGIKDILLSLVDQKVNIYSVRQELLDFLNNDLY
                     KNDGEILSYKESIMLFCAE"
BASE COUNT          376 a          168 c          186 g          330 t
ORIGIN      
        1 aatgataatg atgaaaaagt caaaatcgaa aatgatataa agaaaataga taaaaaaatt
       61 gaagaaatag ataatgcaat ccgggatctt tcctctgaaa cagagaggtt caagataaag
      121 tatcaacaag agaatacact taatgatgta gttcaaaata tcaggacttg gctaaaagaa
      181 aatcaaaata tggtgaaagc tattaagaaa attgatactg aataacatgg cgaataaata
      241 aacggatcaa gtcgttccta tgacaatgag gttatacgaa aagacgctta ctcatcagaa
      301 atctggtatg ctttaattaa accagtgtcg ctgatgtgta agcgttgacg ttgggagacg
      361 attaaatggt tataccactc ctacgtcttt tccatacggc gtataagagt gcaacatgtt
      421 gagttttccg agtgaatggt gcattttact aaccgagcca tttgaatcaa ccaacgcaat
      481 aaaaaagact ggaataataa gcctgtattt attatttatc gcaatcgcac tttacggttt
      541 tatccaactg aaaagagact gcttccagcg gttgtggatt atccctgggc gatagtcata
      601 gtctggtgtc aggattcaca aatgacctgt ttccttacat ggatataaac acaacataga
      661 gtgcggtagt taatctgaat aatctaattt cggctattca gcacaaaaca acattattga
      721 ttctttataa gagagaatct ctccatcatt tttatataaa tcattattaa gaaaatccag
      781 aagctcttgc cttacactat aaatattgac tttttggtca accaacgaca gtaatatatc
      841 cttgatacct tttaatttca tatttctatc agatattgta ttaaaagtga agatgatatt
      901 acgtttatta caactggaga accaatattt tagagtatta ttcgtccact ttgttggtgt
      961 cttaattagt tgcgctgttg ttgtttttac attgttaaat gtagaaccgc taaaatttgt
     1021 cccgacgcac gaaatgaaat tcatctctga attgttaaaa
//
LOCUS       DAZFAB010000329         1057 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZFAB010000329 DAZFAB010000000
VERSION     DAZFAB010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.334"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(476..>1057)
                     /locus_tag="ACHJWA_005122"
     CDS             complement(476..>1057)
                     /locus_tag="ACHJWA_005122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309587.1"
                     /note="secreted by salmonella pathogenicity island 1;
                     involved in survival of Salmonella in host cells;
                     interferes with host cell ubiquination pathway; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HFO4311517.1"
                     /translation="ESFPIFSIPYHKAFSQNFVSGILDILISDNELKERFIEALNSNK
                     SDYKMIADDQQRKLACVWNPFLDGWELNAQHVDMIMGSHVLKDMPLRKQAEILFCLGG
                     VFCKYSSSDMFGTEYDSPEILRRYANGLIEQAYKTDPQVFGSVYYYNDILDRLQGRNN
                     VFTCTAVLTDMLTEHAKESFPEIFSLYYPVAWR"
BASE COUNT          390 a          221 c          137 g          309 t
ORIGIN      
        1 tgaatatcaa ttcataaaat atgattctcc acgatttgcc cagaaataac gaacataatt
       61 ttcgttgtaa ttaaaatcat atttcatcta tagtgaccat tcacagcaaa acaaaatcaa
      121 ttatcacatt caaaaaaata ctgacaacaa agatactctt cagacaaagt tgtcatatgt
      181 aaattttccc ttaaaacgtt gctcaagaaa aatataaaaa tcatatgccc taatcaacat
      241 cctcaagcac tacgatatat gcagtatcct attcatatat taccaccata tcagacaaat
      301 cgaatcacaa cgagttctat cgtactcttt catcttcgac actgtaaatt ttaagaacat
      361 tcggctcatg ccactaaacc aagacataat aacaacggag caataaatgt tcccactgaa
      421 catgtgcaaa cctaaaaaga cagtgttaca ataatattca tccatggtct ctaaatcaac
      481 gccacgcaac aggataatac aatgaaaata tttcaggaaa agattctttt gcatgctccg
      541 ttagcatatc agtcagcaca gcggtacaag taaaaacatt atttcttcct tgtagcctgt
      601 ctaaaatatc attgtaataa taaactgagc caaatacctg aggatctgtt ttataagctt
      661 gttcaatcaa tccatttgca tatctccgta gaatctcagg agaatcatac tctgtaccaa
      721 acatatcact cgatgagtat ttacagaaaa ccccccctaa acaaaataat atttcagcct
      781 gttttcttag tggcatatct ttcaatacat ggctccccat aatcatatct acatgctgag
      841 cgttcagttc ccaaccatca agaaagggat tccagacaca ggcaagtttc ctttgctgat
      901 catcagcaat cattttataa tctgatttat tggaattaag tgcctcaata aatctctctt
      961 tgagttcatt atcagaaatg agtatatcca gaataccaga aacgaaattc tgggaaaaag
     1021 ctttatgata aggaatactg aaaattggaa atgattc
//
LOCUS       DAZFAB010000330         1049 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZFAB010000330 DAZFAB010000000
VERSION     DAZFAB010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.335"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            31..402
                     /locus_tag="ACHJWA_005123"
     CDS             31..402
                     /locus_tag="ACHJWA_005123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311518.1"
                     /translation="MQKKEIRRLRLKEWFKDKTLPPKEKSYLSQLMSGRASFGEKAAR
                     RIEQTYGMPEGYLDAEYAEQPGGSPPHAGLTSNQLELLQIFSAFPEDEQRQIISELKQ
                     KKESMEDLIARWIAAQKCRRA"
     gene            423..614
                     /locus_tag="ACHJWA_005124"
     CDS             423..614
                     /locus_tag="ACHJWA_005124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stability determinant"
                     /protein_id="HFO4311519.1"
                     /translation="MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDE
                     VERRMAERFAKMRKERSKQ"
     gene            616..894
                     /locus_tag="ACHJWA_005125"
     CDS             616..894
                     /locus_tag="ACHJWA_005125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO4311520.1"
                     /translation="MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLS
                     EHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVEVVNVIHARRQFP"
BASE COUNT          339 a          232 c          237 g          241 t
ORIGIN      
        1 tgtttagtga ttactaaaga taattaccac atgcaaaaaa aagaaattcg ccgtttacgt
       61 ctcaaggagt ggtttaaaga taaaactctg ccacccaaag agaagagcta cctatctcaa
      121 ctaatgagtg ggagagcctc gtttggagaa aaggctgcca gaagaataga gcaaacatac
      181 gggatgccgg aagggtatct ggatgcggaa tacgcagaac aaccgggggg ttctccacca
      241 catgcagggt taacgtctaa tcaactggaa ttattgcaga ttttttcagc cttccctgag
      301 gatgagcaac gccagataat cagcgagtta aagcagaaaa aagaatcaat ggaagatctc
      361 atagcgagat ggattgcggc gcaaaaatgc cgccgcgcct gagttataaa accggaggaa
      421 acatgaatag agccctttca ccaatggttt ctgaatttga aaccattgaa caagaaaaca
      481 gttacaacga atggctgcgt gcgaaagtag caacgagcct tgcagatccg cgcccagcaa
      541 ttccccatga cgaagttgag cgcagaatgg cagaacgctt tgctaagatg cgcaaggaac
      601 ggagcaagca gtaaaatgtt acccgtgtta tggcttgaaa gcgcagatac cgacctagat
      661 gatataacta gttatattgc tcgtttcgac atagatgcgg ctgaacgctt atggcagcga
      721 ttaaggggtt gtgtgctgcc gttatccgaa catccgtatt tatacccacc aagcgacaga
      781 gtacctggct tgcgtgagat tgtagcccac cctaactata taattctata ccgcgtaaca
      841 acatcaagcg ttgaagtagt aaacgtgatc cacgcaagac gccagtttcc ctaactttca
      901 ctaccaatag aaacataaca accgcaacga ctttatcaaa agcgttgtgt ttgttatgtc
      961 ccgcggttta gtttttactt gacttaagtt taatgtttat taaactaaaa ataccaaccc
     1021 accccgcccc acagaacgca gggaaatac
//
LOCUS       DAZFAB010000331         1026 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZFAB010000331 DAZFAB010000000
VERSION     DAZFAB010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.336"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..>1026)
                     /locus_tag="ACHJWA_005126"
     CDS             complement(<1..>1026)
                     /locus_tag="ACHJWA_005126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4311521.1"
                     /translation="GITEAVTVPAREVIHDRFNCFFHPLIGLPPVYAAGLAATQGHHI
                     QENSTSFFRNGGRPSGVIEIPGSITEENAKKLKSNWDSGYTGENAGKTAILSNGAKYN
                     PTTFSPVDAQTVEQLKMTAEIVCSVFRVPAYKIGVGQPPSSDNVEALEQQYYSQCLQT
                     LIESIELLLDEALETGENESTEFDVTTLLRMDSERRMKTLGDAVKNTLLTPNEARKRE
                     NLPPLAGGDALYLQQQNYSLEALSRRDAREDPFASAGKTVSSQLPDGASDGNKAISET
                     EHDAVKAMFRGDTEKMTERELSIIRALGEEFSTVLADLQRTFEGKMASQAQAFEEKLT
                     SLSAVLQK"
BASE COUNT          205 a          322 c          240 g          259 t
ORIGIN      
        1 cttctgtaat accgccgaca gggaagtcag tttctcttca aacgcttgtg cctgcgaggc
       61 catcttcccc tcaaatgtgc gctgtaaatc cgccagcact gtggaaaatt cttctcccag
      121 tgcacgaata atggacagtt cccgttccgt cattttctca gtatcccccc tgaacatcgc
      181 tttcaccgca tcatgctctg tttcactgat tgccttatta ccgtcagatg cgccgtcagg
      241 cagctgtgat gaaactgttt tcccggcaga cgcgaacgga tcctcacggg catcacgacg
      301 ggacagcgcc tccagactgt agttctgctg ctgaagatac agtgcatcac cgccggccag
      361 gggcggcagg ttctcccgtt tacgggcctc attgggcgtg agaagcgtat ttttcaccgc
      421 atcccccagc gttttcatgc gccgctcact gtccattctc agcagcgtgg tgacatcaaa
      481 ttctgtactc tcgttttccc ccgtttccag cgcctcatcc agtaacagtt caatggactc
      541 aatcagcgtc tgcaggcact gggaataata ctgctgctcc agcgcctcca cgttgtcact
      601 ggaaggcggt tgtcccacgc caatcttgta ggccgggaca cggaacaccg aacagacaat
      661 ttcagcggtc atcttcagtt gttccaccgt ctgcgcatcc accggtgaaa acgtcgtggg
      721 gttgtatttt gccccgttgc tcagaatggc cgttttcccc gcattttcgc ctgtataccc
      781 gctgtcccag ttgctcttca gttttttcgc attttcttcc gtaatactgc cggggatctc
      841 aatcaccccg gacggcctgc cgccatttct gaaaaaagac gtcgaatttt cctgaatatg
      901 atgcccctgc gtggccgcca gcccggcggc atacaccggc ggcaatccta taagcggatg
      961 aaaaaaacag ttaaaccggt cgtggatcac ttcccgggca ggcaccgtca ccgcctccgt
     1021 gatccc
//
LOCUS       DAZFAB010000332         1020 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZFAB010000332 DAZFAB010000000
VERSION     DAZFAB010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.337"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..562
                     /locus_tag="ACHJWA_005127"
     CDS             <1..562
                     /locus_tag="ACHJWA_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311522.1"
                     /translation="FSLTPDGKLTAKNADISGSVNANSGTLNNVTINENCQIKGKLSA
                     NQIEGDIVKTVSKSFPRTSTYASGTITVRISDDQKFDRQVMIPPVLFRGGKHENFNSN
                     NQQSYWYSTCRLRVTRNGQEIFNQSTTDAQGVFSSVIDMPAGQGTLTLTFTVSSSGAN
                     NWTPTTSISDLLVVVMKKSTAGISIS"
     gene            629..>1020
                     /locus_tag="ACHJWA_005128"
     CDS             629..>1020
                     /locus_tag="ACHJWA_005128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO4311523.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHARTNVPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYAGDKNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGMAYSRVSTFSGD"
BASE COUNT          265 a          240 c          272 g          243 t
ORIGIN      
        1 attttccctg acaccggacg gaaagctgac tgctaaaaat gcggatatca gtggcagtgt
       61 gaatgcgaac tccgggacgc tcaacaacgt cacgattaat gagaactgtc agattaaggg
      121 gaaactgtca gccaatcaga ttgaaggcga tattgtcaaa acggtcagca agtctttccc
      181 ccgcacgagc acttatgcca gtggcaccat cacggtaaga atcagtgatg atcagaaatt
      241 tgaccggcag gtcatgatac cgccagtgtt attccgcggt ggtaagcatg agaatttcaa
      301 cagtaataac caacagtcat actggtattc aacctgccgg ttaagagtga cccgcaatgg
      361 ccaggagatt tttaatcagt ccacgacgga tgctcagggc gtattttcct cagttataga
      421 tatgcctgcc ggacagggga cgctgacact gacattcacc gtatcttcat caggagcgaa
      481 taactggaca ccaacaacca gtatcagcga tctgctggtt gtggtgatga aaaaatccac
      541 agcaggtatc agtatcagct gaattttata acccagaacg ggcgtcagaa atgacgcctt
      601 ttttattgca gaaaagcgag aggtaattat gcgtaaactt tatgccgcca ttttgtccgc
      661 agccatttgt ctggccgtat ccggtgcgcc tgcatgggca tctgaacatc agtccacgct
      721 gagcgcgggg tatcttcatg cccggaccaa cgttcccggc agtgatgatc tgaacgggat
      781 taacgtgaaa taccgttatg agtttacgga tacgctgggg ctggtgacgt cattcagcta
      841 tgcaggagac aagaatcgcc agctgacccg ttacagcgat acccgctggc atgaagattc
      901 cgtgcgtaac cgctggttca gcgtgatggc ggggccgtct gtgcgcgtga atgaatggtt
      961 cagcgcgtat gcgatggcgg gtatggctta cagccgtgtt tcgacttttt ccggggatta
//
LOCUS       DAZFAB010000333         1017 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZFAB010000333 DAZFAB010000000
VERSION     DAZFAB010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.338"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..703)
                     /locus_tag="ACHJWA_005129"
     CDS             complement(<1..703)
                     /locus_tag="ACHJWA_005129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO4311524.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVSTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFT"
     gene            complement(709..>1017)
                     /locus_tag="ACHJWA_005130"
     CDS             complement(709..>1017)
                     /locus_tag="ACHJWA_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO4311525.1"
                     /translation="VISRWRIEQCSELSAVSASFVLSTPTETDGAVFPGRIMLANTCT
                     WTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNVGNFGGFLSINKLSQ
                     "
BASE COUNT          224 a          323 c          269 g          201 t
ORIGIN      
        1 ccgtaaaggc agatgcgcgc catgcccggt gacgccagag ggagtgtgtg cgtcgctgcc
       61 atttgtcggt atacctctct cgtttgctca gttgttcagg aatatggtgc agcagctcgc
      121 catcaccaca gtaaatggcg gcatgattcg gcaccgatga accaaaacag cacagcagca
      181 catcgcccgg ttgtgctgat gacaacggca cctgatacag ccctgtggcc tccagattat
      241 ccagatagag attctgaccg tgacgccacc agtcatcctc gcgatgaaaa tccggcatct
      301 caatccccgc cagatgataa gcatcccgga acagcgtgta acagtccgtc accccgtgct
      361 caaagcgccg cccggtgaga tgcggcacac agcggaactt atgaatcgtc ccccggcaga
      421 ccagccacca cggcaaatca ctctgcacct gcagccgccg gtcggcctca ctcagccagg
      481 gcagaccacc ggggtggctg tggaccagcg ccacaatctc accctgcatt tctgcctgca
      541 gccagtcttc cggcgacata cggaaatacg cctccggctc accggagata ttcacgcagg
      601 ggaaatatct ttccccctcc ggcgtgctta ccacgaagcc gcacgactcc gctggcgcac
      661 atcgccgggc gtgcgccaga atcgctgatt ctgtctgtgt catgggattt actgcgaaag
      721 tttgttaatg gaaaggaagc cgccaaagtt gccgacgtta ttgcggaact tacaaccgct
      781 caggcatttg ctgcatttat ccttcgtgat atcggacgtt ggctggtcat attcatccgc
      841 gaccgccgga ccgtgataac cgcactcatc gccgcgatag gtccaggtgc aggtgttggc
      901 cagcatgata cgtcccggaa aaacagcgcc gtccgtttcc gtcggcgtgg acagtacaaa
      961 agaggcactc accgcgctca gttcgctgca ctgctcaatg cgccagcggc tgatcac
//
LOCUS       DAZFAB010000334         1007 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZFAB010000334 DAZFAB010000000
VERSION     DAZFAB010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.339"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..251)
                     /locus_tag="ACHJWA_005131"
     CDS             complement(<1..251)
                     /locus_tag="ACHJWA_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFO4311526.1"
                     /translation="MSQPAGDLVIDLSLDAARFDEQMARVRRHFSSLEADARKTASTV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIVTQLA"
     gene            complement(232..645)
                     /locus_tag="ACHJWA_005132"
     CDS             complement(232..645)
                     /locus_tag="ACHJWA_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO4311527.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRRFYCTHYFQDTQLDAHFSGLMY
                     AVLSLFFGDPDMHPADFSLLAPACEEEQTEMPDEEEMLMQKATGVAGGVRFGGDGGRD
                     ISPSADVVDVSEDDVALMMASAGISGGVRYVPASG"
     gene            complement(672..>1007)
                     /locus_tag="ACHJWA_005133"
     CDS             complement(672..>1007)
                     /locus_tag="ACHJWA_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO4311528.1"
                     /translation="KFVSDAEQQETTKHDVVHINQRYLETASLLVAMSLWHSHSLKGT
                     LASPETEMQQIRREVMLGWPADALNQATNRVLYLSGMLDNRHDADPEQTGKAEATESV
                     TSKKHSKAS"
BASE COUNT          220 a          308 c          242 g          237 t
ORIGIN      
        1 gcaagctgag tgacaatatc cgtgaactgt gcgggcagtg tgcgcatggc agccttatac
       61 tgtccgactg atatcccggc tttttgtgcc gccagcgcct gtcggctcag cccctgttca
      121 acagtactgg cggtttttct ggcatccgcc tccagactgg aaaaatgacg gcgtacccgg
      181 gccatctgtt catcaaaccg ggccgcatcc agactcaaat caataaccag atcacccgct
      241 ggctgggaca tatctcacac ctccggaaat ccccgctgaa gccatcatta atgcaacatc
      301 atcctcgctg acatccacca catccgcaga aggtgaaata tcgcgccctc cgtcccctcc
      361 gaaccggacg cctccggcaa ctcctgtcgc tttctgcatc agcatttctt cctcgtccgg
      421 catctccgtc tgctcttcct cacacgctgg agcaagcaga ctgaaatccg ccggatgcat
      481 atccggatcg ccaaaaaaca ggctgagtac ggcgtacatc agcccggaaa aatgagcgtc
      541 cagttgggta tcctgaaaat aatgcgtgca gtaaaaacgt cgccagtcgg catattcggt
      601 ggatgtcatc ccggcaagca tggcgcgcca gtcgggtctc cccatctctc gcgccagttt
      661 caggacaaag ttcagctcgc cttcgaatgc ttttttgatg ttaccgactc agtcgcttct
      721 gctttaccgg tttgttcagg atcggcatcg tgccggttat ccagcatacc tgaaagataa
      781 agcacccggt tcgttgcctg attcagtgca tcagcaggcc atcccagcat cacttcacgg
      841 cggatctgct gcatctctgt ctccggagag gccagagtgc ctttgaggga atgggaatgc
      901 catagcgaca tcgccacaag cagggatgcc gtttccagat atcgctggtt aatgtgcacg
      961 acatcatgct tcgttgtctc ctgttgttct gcgtctgaaa caaactt
//
LOCUS       DAZFAB010000335          998 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZFAB010000335 DAZFAB010000000
VERSION     DAZFAB010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.340"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..284)
                     /locus_tag="ACHJWA_005134"
     CDS             complement(<1..284)
                     /locus_tag="ACHJWA_005134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFO4311529.1"
                     /translation="MAGNFADLTAVLTLDSARFSEEAARVKKELGETSALADLMSGKV
                     SQSFRKQADAAEQSLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIVTQLA"
     gene            complement(334..636)
                     /locus_tag="ACHJWA_005135"
                     /pseudo
     CDS             complement(334..636)
                     /locus_tag="ACHJWA_005135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310194.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
     gene            complement(663..>998)
                     /locus_tag="ACHJWA_005136"
     CDS             complement(663..>998)
                     /locus_tag="ACHJWA_005136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO4311530.1"
                     /translation="KFVSDAEQQKTTKHDVVRINQRYLETASLLVAMSLWHSHSLKGT
                     LASPETEMQQIRREVMLGWPADALNQATNRVLYLSGMLDNRHDADPEPTGKTEATEPV
                     TSKKHSKAS"
BASE COUNT          216 a          300 c          239 g          243 t
ORIGIN      
        1 gcaagctgag tgacaatatc cgtgaactgt gcgggcagtg tgcgcatggc agccttatac
       61 tgtccgactg atatcccggc tttttgtgca gccagcgcct gtcggctcag actctgctca
      121 gcagcatcag cctgttttct gaaagactga ctgactttcc cggacatcaa atcagcaagc
      181 gcactggttt cacccagctc tttttttacc cgcgctgcct cttcagaaaa acgggcagaa
      241 tccagtgtca gcacggctgt cagatcggca aaattacctg ccatcgtggt cacctcctga
      301 aatgtcctct gataccatca ataactgtca caacctcctt ccctcctccc cgaaacggac
      361 tccaccggcg aggcccgccg ccttctgcat cagtacatca ttttcgtccg gcgtctccgc
      421 ctgtccttcc tctgcctccg gagcgaacag gctgaaatcc gccggatgca tatccggatc
      481 gcaaaaaaac aggctgagta cggcgtacat cagcccggaa aaatgcatat ccagctgggt
      541 atcctgaaaa taatgcgtgc agtaaaaacg tcgccagtcg gcatattcgg tggatgtcat
      601 cccggcaagc atggcgcgcc agtcggctct ccccatctca cgcgccagtt tcaggacaaa
      661 gttcagctcg ccttcgaatg cttttttgat gttaccggct cagtcgcttc tgttttcccg
      721 gttggttcag gatcggcatc gtgccggtta tccagcatac ctgaaagata aagcacccgg
      781 ttcgttgcct gattcagtgc atcagcaggc catcccagca tcacttcacg gcggatctgc
      841 tgcatctctg tctccggaga ggccagagtg cctttgaggg aatgggaatg ccatagcgac
      901 atcgccacaa gcagggatgc cgtttccaga tatcgctggt taatgcgcac gacatcatgc
      961 ttcgttgtct tctgttgttc tgcgtctgaa acaaactt
//
LOCUS       DAZFAB010000336          987 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZFAB010000336 DAZFAB010000000
VERSION     DAZFAB010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.341"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            232..>987
                     /locus_tag="ACHJWA_005137"
     CDS             232..>987
                     /locus_tag="ACHJWA_005137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311531.1"
                     /translation="MSIDFTPGMINTYHGDIYNRTTDTDNVKTPDTPMWPCDNREEQQ
                     PINSTFSGEGYNPEQYDLARQHLPQIYACRTNTKYTDADYSKVVAQLVSLITNIESIS
                     STPLTRQAQEILNQINNIRYEKNKSAECRIIVVANPKPDKAIITKISVEEGIPVRFSV
                     QTMFSDTNFIAEQRADLPTNIKDIQSLYQKMTKLYIEHSENKNRMKVFAGTNFIDFNM
                     TGQNLSGFVLTLSRFYFEDLLNINFTDANLGDTI"
BASE COUNT          358 a          186 c          169 g          274 t
ORIGIN      
        1 ctttttaaaa tgagcgcttt ttgatagttg ttaacgtttt cctttacgat actatcaatg
       61 ccctttttta tacacgaata ttaccgggtg gagataaaag ggaagtcaaa ggtaattata
      121 tcaggaagat atgacgcatg atatttattg tgcgtagaag aaaatccatt cctgctttaa
      181 ttatccaatt taaaaatctt agcataaaac agataatgaa taaggaatgt tatgtcaatt
      241 gactttaccc caggtatgat aaatacatat cacggcgata tttataaccg cacaacagat
      301 accgacaatg ttaaaacacc agacacaccg atgtggcctt gtgataatcg ggaagaacaa
      361 caacccatta attccacatt ctctggagaa ggatacaacc ctgaacaata tgatttagcg
      421 cggcaacacc tcccacaaat ttatgcgtgc cgcacgaaca ccaaatatac agatgcagac
      481 tactcaaaag ttgtggctca acttgtaagt cttattacta acattgaatc gataagttcg
      541 acaccactta cgcggcaggc acaggaaata cttaatcaaa ttaataacat tagatacgaa
      601 aagaacaaaa gtgctgaatg tcgaatcatt gttgtcgcta atcctaaacc cgacaaggca
      661 atcataacta aaattagcgt agaagaggga ataccagtaa gattcagtgt ccaaaccatg
      721 ttttcagata ctaattttat tgctgaacaa cgagctgact taccgactaa tataaaagat
      781 attcaaagcc tgtatcaaaa aatgacgaag ttatatattg agcacagtga aaataaaaac
      841 aggatgaaag tctttgccgg aaccaacttt attgatttca atatgacagg acagaacctg
      901 tccgggttcg ttctaacctt gtcaaggttt tatttcgaag atctacttaa tataaatttc
      961 actgacgcaa atttaggtga cactatt
//
LOCUS       DAZFAB010000337          977 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZFAB010000337 DAZFAB010000000
VERSION     DAZFAB010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..977
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.342"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..126
                     /locus_tag="ACHJWA_005138"
     CDS             <1..126
                     /locus_tag="ACHJWA_005138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO4311532.1"
                     /translation="QGNPMPVPYGEMLVGSRRISQDISTRDEGGGGTVVVVGRQG"
     gene            469..>977
                     /locus_tag="ACHJWA_005139"
     CDS             469..>977
                     /locus_tag="ACHJWA_005139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4311533.1"
                     /translation="MGKGGGKAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETGLGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLLETTSKGDRNHSSVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQFLASVIL"
BASE COUNT          254 a          218 c          301 g          204 t
ORIGIN      
        1 caggggaacc cgatgccggt gccttacggg gaaatgctgg ttggctcccg ccgtatatcc
       61 caggacatca gcacccgtga tgaaggcggg ggcggaacgg tcgtggttgt cgggcgacag
      121 ggataaaaca taaaaaaatc ccgcagtgat cgcggagctg cggggacaga caaatgaaga
      181 tcaatgttaa ggagttgttt ttgttactcg ggcaaaaaaa cactaacgca gcgaaattat
      241 aagcgccaca gtcagtgtgt gaaaatgtga agatattcag aatttttatg ccattaccgg
      301 ttttaaccaa caggattatc ggtgggcatg aaagaaaacc ccggtatctg ctgataccgg
      361 ggtttctctt tagcatggca gaaatgtgtt tcatgctttt cgggcgaagg atatccgact
      421 tctgtacgga atggcaagtg gcggttaatt tattcagggg aaggctgtat gggaaaaggt
      481 ggcggtaagg cacacacgcc tcgtgaggcg aaggataatc tcaaatccac gcagatgatg
      541 agtgtgattg atgcgattgg tgagggaccg atagaaggtc cggtgaaggg actgcagagt
      601 attctggtga acaaaacccc actgacggac acggacggca atcccgtgat acacggtgtg
      661 accgcggtct ggcgtgccgg ggagcaggag cagacaccac cggaaggctt tgagtcctcc
      721 ggagctgaaa ccggactggg cgtggaagtg acgaaggcaa aaccggtgac gcgcaccatt
      781 acgtccgcga acattgaccg cctgcgggtc accttcgggg tgcagtcact gttggagacc
      841 acctcaaagg gcgaccgtaa tcactcttct gtccgactgc tgattcagtt gcagcgtaac
      901 ggtaactggg tgacggaaaa ggatgtcacc attaacggca agaccacctc gcagtttctg
      961 gcgtcggtga ttctgga
//
LOCUS       DAZFAB010000338          974 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZFAB010000338 DAZFAB010000000
VERSION     DAZFAB010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.343"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>974
                     /locus_tag="ACHJWA_005140"
     CDS             <1..>974
                     /locus_tag="ACHJWA_005140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS66-like element ISEc8 family transposase"
                     /protein_id="HFO4311534.1"
                     /translation="HALQEKLSNREREIDHLQAQLDKLRRMNFGSRSEKVSRRIAQME
                     ADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPFPESLPRDEKRLLPAAPCCPNCGG
                     SLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQCDAIVQAPAPSRPIERGIAGPGLL
                     ARVLTSKYAEHTPLYRQSEIYGRQGVELRRSLLSGWVDACCRLLSPLEEALHGYVMTD
                     GKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNAGSALAPAVWFAYSPDRKGIHPQT
                     HLACFSGVLQADAYAGFNELYRNGGITEAACWAHARRKIHDVHVRIPS"
BASE COUNT          203 a          283 c          305 g          183 t
ORIGIN      
        1 ccacgccctg caggaaaagc tgagcaaccg ggagcgcgaa atagaccatc tgcaggcgca
       61 gctggataaa ctccgccgga tgaacttcgg cagtcgttcc gaaaaagtct cccgccgtat
      121 cgcacaaatg gaagccgatc tgaaccggct tcagaaagag agcgatacgc tgactggtag
      181 ggtgtatgac ccggcagtac agcgtccgtt gcgtcagacc cgcacccgta agccgttccc
      241 tgaatcacta ccccgtgacg aaaagcgact gttgcctgcg gcgccgtgct gcccgaactg
      301 cggcggttca ctgagctatc tgggcgagga taccgccgaa cagctggagt tgatgcgtag
      361 cgccttccgg gttatccgga cggtacggga aaaacatgcc tgtactcagt gcgatgccat
      421 cgtgcaggca cctgcacctt cgcggcccat cgagcggggt atcgccggac cggggctgct
      481 ggcccgcgtg ctgacctcga agtatgcaga gcacaccccg ctgtatcgcc agtcagaaat
      541 atacggccgg caaggtgtgg agctgaggcg ttcactgctg tcgggctggg tggatgcatg
      601 ctgccggctg ctgtctccgc tggaagaggc gcttcatggc tatgtcatga ctgacggcaa
      661 actccatgcc gatgataccc cggtccaggt actgctgccg ggtaataaga agacgaagac
      721 cgggcggttg tgggcgtatg ttcgtgatga ccgcaatgca gggtcagcgt tggcacctgc
      781 agtgtggttc gcttacagcc cggacagaaa aggcatccat ccgcagactc atcttgcctg
      841 cttcagcggt gtgctgcaag cggatgcgta cgccgggttc aacgagctgt atcgcaatgg
      901 tgggataacg gaagctgcct gctgggctca tgcccgccga aagatccacg atgtgcacgt
      961 ccgcatcccg tcag
//
LOCUS       DAZFAB010000339          974 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZFAB010000339 DAZFAB010000000
VERSION     DAZFAB010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.344"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            148..483
                     /locus_tag="ACHJWA_005141"
     CDS             148..483
                     /locus_tag="ACHJWA_005141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311535.1"
                     /translation="MVISTVGYEKRTGSASSASIIIQKELQPDNAIIQGKAISSVGRN
                     VEISTGKRGTDINTRRIPASNCLSARNAELKHNHTGARILWSYAFSIVTTNRNKNLAG
                     ANIKRCDTV"
     gene            complement(793..921)
                     /locus_tag="ACHJWA_005142"
     CDS             complement(793..921)
                     /locus_tag="ACHJWA_005142"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311536.1"
                     /translation="MGADVTEFSVQGKSGTCRQFSDLFNREIISYSLSERSVMEHR"
BASE COUNT          280 a          199 c          206 g          289 t
ORIGIN      
        1 tataataatt atcatatatg gttattttca tattgatttc gtcagaaata gaaaatgctg
       61 atgatgaatt gtgaataatc aaactgacct ctaattatcc tgtatggagt agtgcttatt
      121 ttcgactcaa tttctactga ttagattatg gttatcagta cagtgggtta tgaaaaacgg
      181 actggatctg ccagcagtgc ttctattatt attcaaaaag aacttcagcc ggacaatgct
      241 ataatccagg gaaaggctat aagctctgtt ggacggaatg tggaaatcag cactggtaaa
      301 aggggaactg acattaacac aagaaggatt cctgcaagca actgcttatc tgcccggaac
      361 gcagaattaa agcataacca cacaggagcc agaatactgt ggtcgtacgc attcagtatt
      421 gtgacaacta atagaaataa aaatctggcc ggtgcgaata ttaaacgatg cgataccgta
      481 tagtgtaagt caaaagtaac ggggtattta aaccatcttt ttatatttat ttaacataat
      541 gcagattctg atgtattagt tcagttgttc gccagaatca gcgctcgatc actatgcgtt
      601 ttagcagtgt gctgaagaag ttcttcatca ctgcgttatt cagaccgttt cctttgtgcg
      661 gcatattttg tgctactcca tggggcttta actttgccta tagcctgcaa ttcgatattg
      721 ccaaccctga tccatatgca gcgacggggc atcctctggc cgggcactgt gaatgcatcg
      781 tatagcatcg tattaacgat gctccatcac cgacctttct gacaagctgt aggagataat
      841 ttcccggtta aacagatcgg agaactggcg acaggtacca cttttcccct gtaccgagaa
      901 ctctgtaaca tccgctccca tttttcgttg gcctttgtca aagttccggc tcaggatatt
      961 gggcgcagat cact
//
LOCUS       DAZFAB010000340          973 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZFAB010000340 DAZFAB010000000
VERSION     DAZFAB010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..973
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.345"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>973
                     /locus_tag="ACHJWA_005143"
     CDS             <1..>973
                     /locus_tag="ACHJWA_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO4311537.1"
                     /translation="TFGVQSLLETTSKGDRNHSSVRLLIQLQRNGNWVTEKDVTINGK
                     TTSQFLASVILDNLPPRPFNIRMVRETADSTTDQLQNRTLWSSYTEIIDVKQCYPNTA
                     IVGLQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAW
                     CLWDMLTHPRYGMGKRLGAADVDKWALYAIAQYCDQMVPDGFGGTEPRMTFNAYLSQQ
                     RKAWDVLSDFCSAMRCMPVWNGQTLTFVQDSPSDVVWPYTNSDVVVDDNGVGFCYSFS
                     ALKDRHTAVEVNYTDPQNGWQTSTELVEDPEAILRYGRNLLKMDAFG"
BASE COUNT          217 a          263 c          308 g          185 t
ORIGIN      
        1 tcaccttcgg ggtgcagtca ctgttggaga ccacctcaaa gggcgaccgt aatcactctt
       61 ctgtccgact gctgattcag ttgcagcgta acggtaactg ggtgacggaa aaggatgtca
      121 ccattaacgg caagaccacc tcgcagttcc tggcgtcggt gattctggat aatctgccgc
      181 cccggccctt taacatccgg atggtcaggg agacggcgga cagcaccacg gaccagctgc
      241 agaacagaac gctctggtcg tcatacaccg aaatcatcga tgtgaaacag tgctacccga
      301 acacggcgat tgtggggctg caggtggatg cggagcagtt tggtggtcag cagatgacgg
      361 tgaactacca tatccgcggt cgcatcatcc aggtaccgtc aaactatgac ccggaaaaac
      421 gcacgtacag cggcatctgg gacggcagcc tgaaaccggc atacagcaac aaccctgcct
      481 ggtgcctgtg ggacatgctg acccacccgc gctacggaat gggaaaacgc ctgggggcgg
      541 cggatgtgga caagtgggcg ctgtatgcca ttgcgcagta ctgcgaccag atggtcccgg
      601 atggtttcgg gggcacagag ccgcggatga cctttaatgc gtacctgtca caacagcgta
      661 aggcgtggga cgttctcagt gatttctgct cggcgatgcg ctgtatgccg gtatggaacg
      721 gccagacgct gacgttcgtt caggacagtc cgtcggatgt ggtgtggccg tacaccaaca
      781 gtgatgtggt ggtggatgat aacggcgtgg ggttttgcta cagcttcagc gccctgaagg
      841 accgccacac ggcggtggag gtgaattaca ccgacccgca gaacggctgg cagacctcca
      901 cggaactggt ggaagacccg gaagccatac tgcgctacgg gcgcaacctg ctgaagatgg
      961 atgcgttcgg ctg
//
LOCUS       DAZFAB010000341          968 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZFAB010000341 DAZFAB010000000
VERSION     DAZFAB010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..968
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.346"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            143..427
                     /locus_tag="ACHJWA_005144"
     CDS             143..427
                     /locus_tag="ACHJWA_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HFO4311538.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAWADSLSTDNPLPVPR"
     gene            427..702
                     /locus_tag="ACHJWA_005145"
     CDS             427..702
                     /locus_tag="ACHJWA_005145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO4311539.1"
                     /translation="MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIYVLRLWHTRENR"
     gene            797..>968
                     /locus_tag="ACHJWA_005146"
     CDS             797..>968
                     /locus_tag="ACHJWA_005146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311540.1"
                     /translation="MNYDEITKITAERISDYMTEAVNTDSIAVAEMFHNAAWGVRTLW
                     FELVTKIDIDIHK"
BASE COUNT          275 a          213 c          238 g          242 t
ORIGIN      
        1 gcttacgccg ggcttcggcg gttttacacc tgtgctacat gtaacctccg tataccgcct
       61 tacatgcgct gacgcgcatt atttgccttg ttccgtctga aagtaatcac tatgattaaa
      121 tatgattaac gactaatcgg atatgcaaat gaaaaacaat accgcacaag caacaaaagt
      181 cattaccgcg catgtgccat tacctatggc tgataaagtc gaccagatgg ccgccagact
      241 ggaacgctcc cggggttggg ttatcaaaca ggcgctttct gcatggcttg cccaggagga
      301 ggagcgtaat cgcctgacgc tggaagccct ggacgatgtg acatccggac aggttattga
      361 ccatcaggct gtacaggcct gggcggacag cctcagtact gacaatccgt taccggtgcc
      421 acgctgatgg aactgaagtg gagcagtaag gcgctttctg atttggcgcg gttatatgat
      481 tttctggtgc tagccagtaa acctgctgcc gccagaacgg tacagtccct gacacaggca
      541 ccggtcattc tgttaactca tccacgtatg ggggaacagt tatttcagtt tgaacccagg
      601 gaggtcagac ggatttttgc tggcgagtac gaaatccgtt acgaacttaa tggccagact
      661 atttatgtat tgcgcctgtg gcacacacga gaaaacaggt agattgtgtg tggggacaac
      721 ttaacatagc ctgcagatat taattctgga acattattat caggccagat atttaaataa
      781 tcttgtagga gataaaatga attacgatga aataacgaaa attaccgctg aacgtattag
      841 cgactacatg actgaagcag ttaatacaga ctctatagcc gttgcggaaa tgtttcataa
      901 cgcagcatgg ggagtgcgaa cattgtggtt tgaactcgtc acaaaaatag atatagatat
      961 acataaaa
//
LOCUS       DAZFAB010000342          962 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZFAB010000342 DAZFAB010000000
VERSION     DAZFAB010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..962
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.347"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>962
                     /locus_tag="ACHJWA_005147"
     CDS             <1..>962
                     /locus_tag="ACHJWA_005147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4311541.1"
                     /translation="DGVETYGVWGLSLPSLRRRLFRCVSIRENTDGTFAITAVQHVPE
                     KEAIVDNGAHFDGDQSGTLNSVIPPAVQHLTVEVSAADSQYLAQAKWDTPRVVKGVRF
                     SLRLTSGSGQDSRLVTTAITADTEHRFSGLPLGEYTLTVRAINSYGQQGEPAITTFRI
                     NAPAKPATIELTPGYFQITAVPVLAVYDPTVQFEFWFSEKRITNTAQVEKSARYLGSG
                     SQWTVQGSRIKPGTDFWFYVRSVNLVGKSAFVEVSGQPSNDGEGYLEFFREKIGKLHL
                     AQGLWELIDNSQLADEMAEMKTTITETRNEITQTVSKTLENQSAT"
BASE COUNT          228 a          252 c          308 g          174 t
ORIGIN      
        1 gatggcgtgg agacatacgg tgtgtggggg ctctccctgc cgtcactgcg tcgtcgcctg
       61 ttccgctgtg tctccatccg ggaaaacacg gacggcacct ttgccatcac ggcagtgcag
      121 cacgtaccgg aaaaagaagc catcgtggat aacggggcgc actttgacgg cgaccagagc
      181 ggcaccctga acagcgtcat ccctccggca gtgcagcacc tgacggtgga ggtgagtgca
      241 gctgacagcc agtatctggc gcaggcgaaa tgggacacgc cgcgggtggt gaagggcgtg
      301 cgcttcagtc tgcgcctgac cagtggaagc ggtcaggaca gccgtctggt gaccaccgcc
      361 atcactgcgg atacagagca tcgtttcagt ggtctgccgc tcggggaata caccctgaca
      421 gtcagggcaa ttaacagtta tggccagcag ggggaaccgg ccatcaccac cttccggatt
      481 aacgcgccag caaaacccgc caccattgaa ctgacgccgg ggtattttca gataacggcg
      541 gtaccggtgc tggcggtgta tgacccgacg gtgcagtttg agttctggtt ttcggaaaaa
      601 cgcatcacga acacggcaca ggtggaaaaa tctgcccgtt atctggggag cggcagtcag
      661 tggactgtcc agggaagccg gattaagccg gggacggatt tctggtttta cgtgcgcagc
      721 gtcaacctgg tggggaaatc tgcgtttgtg gaagtcagcg ggcagcccag caatgatggt
      781 gaagggtatc tggaattttt ccgggaaaaa ataggaaaac tgcatctggc tcaggggcta
      841 tgggagctga tagacaacag ccagcttgcg gatgagatgg cggagatgaa gaccaccatc
      901 acggaaaccc gcaatgaaat cacacagacg gtcagtaaaa cgctggagaa ccagagcgcc
      961 ac
//
LOCUS       DAZFAB010000343          959 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZFAB010000343 DAZFAB010000000
VERSION     DAZFAB010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.348"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..623)
                     /locus_tag="ACHJWA_005148"
     CDS             complement(<1..623)
                     /locus_tag="ACHJWA_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311542.1"
                     /translation="MDNLIGTPPNHAVPHNYIDMEQMEYLCHLNRFSKLSNDFLINGP
                     REDLFTYKYVLDGSFSNLQHLLPKGQLQKIQQRLSSLMHKNMFHCFHVFLLKLCSIES
                     IPLPNADYAFFDDEMPFTLTDEQIENISFLNAYHKEKKGNNDIVTFDFMSADHHYNFS
                     TTIALTNDSFHISSINNHNSQVIFDENIHLHPYELPESSQWCYQLIK"
BASE COUNT          325 a          140 c          167 g          327 t
ORIGIN      
        1 ttttttatta actgataaca ccattgtgat gattctggta attcataggg atgtaaatgg
       61 atgttttcat cgaatataac ctgtgagtta tggttgttta ttgaagatat atggaagcta
      121 tcatttgtta aagctatggt tgtgctaaaa ttatagtggt gatcagcgct catgaaatca
      181 aatgtaacaa tgtcgttgtt tcctttcttt tctttgtgat atgcgtttaa aaaagaaata
      241 ttttcgatct gttcatcagt taatgtaaat ggcatttcgt catcaaagaa agcgtaatcg
      301 gcatttggga gaggaatact ctcaattgag catagtttta ataaaaaaac atgaaaacaa
      361 tgaaacatgt ttttatgcat aagagaactt aatctctgtt gtatcttctg tagttgtcct
      421 ttaggaagta aatgctgtaa atttgaaaat gaaccatcta aaacatattt atatgtaaaa
      481 aggtcctctc ttggaccatt tattaaaaaa tcgttagaca atttagaaaa acgattgaga
      541 tgacataaat attccatttg ctccatgtct atatagttat ggggaactgc gtggtttggt
      601 ggtgtgccaa taagattatc cataaatcac cttgataaaa tttaataaaa tctctttatt
      661 ttaacctcga caataaaagc ggctataaaa aaacgctcat tttctgatgc tgcggttaac
      721 ccgtggaatg taccgttaac ccccaaaaaa ttgcaaatcc agggtgaaca ccctgggtta
      781 aaatggaaat aatgagctat tttttacagt attcaccggt aatataagta acacttgttg
      841 ctaacctaac tagcagtaca gttaattaac aacatagaag ttattgtttt tcaaacaact
      901 caatattaca cgccggagga tttatggtcg ctaaattaaa accagatgtt tttgttaat
//
LOCUS       DAZFAB010000344          932 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZFAB010000344 DAZFAB010000000
VERSION     DAZFAB010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.349"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..930
                     /locus_tag="ACHJWA_005149"
     CDS             <1..930
                     /locus_tag="ACHJWA_005149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO4311543.1"
                     /translation="RLERELRQGSVSEESQQWLAQCGLTAEQMAAQLEAEYIPERKLH
                     LYHCDHRGLPQALISPEGETAWQGEYDEWGNLLGETSAQHLQQPYRLPGQQYDEESGL
                     YYNRHRYYDPLQGRYITQDPIDIKGGWNLYSYALNPVSWIDPLGLTQCDSEGCNNDIL
                     FTGGSGPDNKILNELGPRDGIDGLGSQNMKMYSGLLGGDILKPGILGGLTLGSVTQRP
                     SRTAEEEVQAQIEYLAYKKRCEQKPDSNLSRCAAAIFQKSRKEDCLRMRQEWDDKWWP
                     GKHADEIKNVKASLKNTLIDVKKRCMPTSGIPR"
BASE COUNT          280 a          173 c          282 g          197 t
ORIGIN      
        1 aggctggagc gggaactgcg gcagggcagc gtgagtgaag aaagccagca gtggcttgcg
       61 cagtgcgggc tgacggcgga acagatggcc gcgcagctgg aggcggaata tatcccggag
      121 cggaaacttc atctttacca ctgcgaccac cggggactgc cgcaggcgct catcagcccg
      181 gaaggggaaa cggcgtggca gggggagtat gacgagtggg gaaacctgct gggcgaaacc
      241 agcgcgcagc acctgcaaca gccgtaccgt ctgccgggac agcagtatga tgaggagtcg
      301 gggctgtatt acaaccgtca ccggtactat gacccgctac aggggaggta tatcacccag
      361 gatccaatag atataaaagg aggatggaat ttatattctt atgcgcttaa tccggtaagt
      421 tggattgacc cattaggatt gacgcagtgc gattccgaag ggtgtaataa tgatatatta
      481 tttacaggag gaagtggtcc cgataataaa atacttaatg aattaggtcc gagagatggc
      541 attgacggtt tgggttcaca aaacatgaaa atgtatagtg ggctattagg gggcgatatt
      601 ttaaaaccgg ggattctggg cggtttgacg cttggtagtg tgacacaacg accatcgcga
      661 acggctgagg aggaggtgca agctcaaata gaatatttag catataagaa gcgttgtgag
      721 caaaaacctg atagtaattt aagtcgttgt gctgccgcga tctttcaaaa gagcagaaaa
      781 gaagattgtt taagaatgag acaagaatgg gatgataaat ggtggcctgg caaacatgcg
      841 gatgaaataa aaaatgtgaa agcatcgttg aaaaacacac ttattgatgt taaaaaaaga
      901 tgcatgccaa ctagtggcat tccgagatag gt
//
LOCUS       DAZFAB010000345          931 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZFAB010000345 DAZFAB010000000
VERSION     DAZFAB010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.350"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>931
                     /locus_tag="ACHJWA_005150"
     CDS             <1..>931
                     /locus_tag="ACHJWA_005150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO4311544.1"
                     /translation="WYYVIVLAGQSNAMAYGEGLPLPDSYDAPDPRIKQLARRSTVTP
                     GGAACRYNDIIPADHCLHDVQDMSTLNHPKADLSKGQYGCVGQGLHIAKKLLPYIPNN
                     AGILLVPCCRGGSAFTQGAEGTFSADAGASQDSARWGVGKPLYQDLIARTKAALQKNP
                     KNVLLAVCWMQGEFDMSAATHAQQPALFTAMLAQFRADLSVFNAQCHGGSAADVPWIC
                     GDTTYYWKNTYGTQYNTIYGAYKNRESEGVYFVPFMTDGNGVNTATNAPAEDPDIPAS
                     GYYGAASRTNGNQVSSNRPTHFSSWARRSIIPDR"
BASE COUNT          215 a          241 c          278 g          197 t
ORIGIN      
        1 ttggtactac gtcatcgtac tggccgggca gtccaatgcc atggcttacg gtgaagggct
       61 tccgctgccg gattcatacg atgctccgga tccgcgcatt aaacagctgg cgcgccgcag
      121 tacagttacg ccgggtgggg ctgcctgcag atataacgat attattccgg ccgaccactg
      181 cctgcatgat gtgcaggata tgagtacgct gaatcatccg aaggcagacc tgagcaaagg
      241 gcagtacggc tgtgtcggcc agggcttaca tattgccaaa aaactgcttc cgtatatccc
      301 gaataacgcg gggatcctgc tggtaccatg ctgtcgtggt ggttcggcat tcacccaggg
      361 cgcggagggg acattcagtg cggacgcggg ggccagccag gattcggcgc gctggggtgt
      421 gggtaaaccg ttatatcagg acctgattgc gcgcactaaa gctgcattac agaagaaccc
      481 gaaaaatgtg ttgctggcgg tgtgctggat gcagggagag tttgacatga gcgccgccac
      541 ccacgcacag caacctgcgc tgtttacagc catgctggca cagtttcgtg ctgacctctc
      601 cgtgtttaac gcgcagtgcc atggtggcag tgctgcagat gtgccgtgga tttgtggtga
      661 cacgacgtat tactggaaaa atacctacgg cacccagtac aacaccattt acggggcgta
      721 caaaaacagg gagagtgagg gcgtttattt tgtgcccttc atgacagacg gtaacggcgt
      781 caataccgcc actaacgcgc cggcagaaga tccggatatt ccggcatcag gatattacgg
      841 tgcggcatcg agaacgaatg gaaaccaggt atcatcaaac cgcccgacac atttcagttc
      901 atgggcgcgc aggagcatta ttccggatcg t
//
LOCUS       DAZFAB010000346          922 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZFAB010000346 DAZFAB010000000
VERSION     DAZFAB010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..922
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.351"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..201
                     /locus_tag="ACHJWA_005151"
     CDS             <1..201
                     /locus_tag="ACHJWA_005151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO4311545.1"
                     /translation="DDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEK
                     HGSQVDLIFRFLDHAFATGVLG"
     gene            214..588
                     /locus_tag="ACHJWA_005152"
     CDS             214..588
                     /locus_tag="ACHJWA_005152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO4311546.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFVSKAGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVRGK
                     PVSGGRLGVKIYPIMLEGQVKK"
     gene            585..>922
                     /locus_tag="ACHJWA_005153"
     CDS             585..>922
                     /locus_tag="ACHJWA_005153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO4311547.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAA"
BASE COUNT          236 a          208 c          263 g          215 t
ORIGIN      
        1 gacgatccgc atcacctgat tggtcatggt cagggcggaa tggggacaaa atctcacgat
       61 attttcacgc taccgctgtg tcgggagcat cacaacgagc ttcatgcgga tccgctggcg
      121 ttcgaagaaa agcatggttc tcaggttgat ttaatttttc gttttcttga tcacgccttt
      181 gcaaccggcg tgctcgggta aaagaggtga ctgatgctca tagatttggt tttaccttac
      241 ccgccgacgg tgaacactta ctggcgacgc cgtggcagca catattttgt atcaaaagcc
      301 gggaagcgtt atcgccgggc agtggcgctt attgttcgcc agcagcgact gaaattaagc
      361 ctgtccggac ggctggcaat aaaaattatt gcagagccac cggataagcg ccgccgcgac
      421 ctggacaata tcctgaaagc accgctggat gcgctgacgc acgcggggtt gttaatggac
      481 gatgagcagt ttgatgaaat caatattgta cgtggaaagc cagtatctgg tggacgtctg
      541 ggggtgaaga tttaccccat aatgcttgaa gggcaggtca aaaaatgaaa ctggaagatt
      601 taccgaaata ctactcccca aaatcccctg gcctgaccga tgcatcggcc tcaacgtcaa
      661 aagatgcgct gagtatcact gatgtgatgg ccgcgcaggg catgacacag aatcgggctg
      721 agatggggtt ttctgcgttc cttgggaaaa tgggcattag tatgaatgac agagagcggg
      781 caacagaatt gctgacagaa tatgcactca gtcggtgtga tcgcgtggcg gcgttaagaa
      841 aactcccggc agaaataaaa ccggcagtga tgcgtattat ggcttcgtat gcgtttgaag
      901 attatgcccg tagcgcggcg ag
//
LOCUS       DAZFAB010000347          914 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZFAB010000347 DAZFAB010000000
VERSION     DAZFAB010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..914
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.352"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            278..585
                     /locus_tag="ACHJWA_005154"
                     /pseudo
     CDS             278..585
                     /locus_tag="ACHJWA_005154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(825..>914)
                     /locus_tag="ACHJWA_005155"
     CDS             complement(825..>914)
                     /locus_tag="ACHJWA_005155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspF"
                     /protein_id="HFO4311548.1"
                     /translation="PPPTGPSGLPPLAQALKDHLAAYEQSKKG"
BASE COUNT          281 a          205 c          176 g          252 t
ORIGIN      
        1 caaaacccct tttaatccct tatatatcag gctttctttg tgtacagggg tcaaccgact
       61 caacctgtat caagttgctt ttgaggatac tttagggggt attatttcct cttgaaatat
      121 tagcacccca taatgggaaa aaggtaatac ataacaatgg tttttaacag tatctttgtt
      181 atacaagggg gcattgagga tatcaggaaa aatctcaaaa tcggtacgga caggaaacta
      241 cgtgggcagc aaaaacgtat ggtaacaccg ggaaatgcaa cgcccaccag tcgggctaat
      301 ccaccactcc aatcgtggtt cgcagtactg cacacacgat taccgggcta tacaggagcg
      361 gtttggtctg aaaacatcca tgccgcgtaa aggtaaccgt tacgacaaca cctcaatgaa
      421 aaacttatag taaacgctga agaatgaaac tatcgtttta ataaccggga tgaagccatc
      481 taagtaatac ggaaatacat tgagattttc tacaaccatc agcgccgcca ctcccgtctg
      541 gggaatatct ccctggtagc cttcaggaaa aatataacca gataactgcg taaaaaaaga
      601 acaaatggtg gtgtccgcta ttgccagtat acctgagttt actgcctttc agattatcac
      661 aatcaatagc gaacagactt ttaagctctg tgcgctttta acaaaacgtc cctttctcca
      721 ctttcaccgt ataataatac attatttatt acccctccaa taaaaaccga tattactaat
      781 cacatttatt accacacaat ttgatatcgg tttttattag ttggttaccc tttcttcgat
      841 tgctcatagg cagctaaatg atcttttaat gcctgtgcaa ggggcggtag tccactaggc
      901 cctgtcggtg gcgg
//
LOCUS       DAZFAB010000348          906 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZFAB010000348 DAZFAB010000000
VERSION     DAZFAB010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.353"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>906
                     /locus_tag="ACHJWA_005156"
     CDS             <1..>906
                     /locus_tag="ACHJWA_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO4311549.1"
                     /translation="GYINRSRATGVSKGPGQQEAVSRLAEELTGKKHTSPKTRSAGER
                     EEEQAREALLALEAELRTLEKHSGANEKISRQRRDLWKAESQYAVLKEAATKRQLSWQ
                     EKSLLAHEKETLEYKRQLADLGDKVEHQKRLNELAQQAARFEQQQGAKQAAISAQARG
                     LTDRQAQRESEEQRLRDVYGDNPDALAKATSALKNTWSAEEQLRGSWMAGLKSGWGEW
                     AESATDSFSQVKSVATQTFDGIAQNMAAMLTGSEQNWRGFTRSVLSMLTEIFLKQAMV
                     GIVGSIGSAIGGAFGGGASASSGTAI"
BASE COUNT          217 a          222 c          315 g          152 t
ORIGIN      
        1 gggtacatca accgctcgcg tgcaacaggc gtcagcaaag gtccagggca gcaggaagcc
       61 gtcagccgtc tggctgaaga gctgacaggt aaaaagcata catcaccgaa aacgcgctct
      121 gccggggaga gggaagagga gcaggcaaga gaggctctgc ttgcccttga agctgagctc
      181 aggacgctgg aaaaacacag cggtgcgaat gagaaaatta gccggcagcg ccgtgattta
      241 tggaaggcgg aaagtcagta tgcggtcctg aaagaggctg ccacgaaacg gcagttatcc
      301 tggcaggaaa aatccctgct ggcccatgag aaagagacgc tggagtacaa acgccagctg
      361 gctgacctgg gcgacaaggt tgaacaccag aaacggctga atgagctggc acagcaggct
      421 gcgcggtttg agcagcagca gggcgcgaag caggcggcaa tcagtgccca ggcgcggggc
      481 ctcaccgacc gtcaggcgca gcgggagtcg gaagagcagc gccttcgtga cgtgtacggt
      541 gataatccgg atgcgctggc gaaggccaca tcggcactga agaacacctg gtctgcggag
      601 gagcagcttc gtggaagctg gatggccggt ctgaagtccg gctggggcga gtgggcagaa
      661 agtgcgacgg acagtttttc gcaggttaaa agcgtggcca cgcagacctt tgacggtatt
      721 gcacagaata tggcagcgat gctgaccggc agcgaacaga actggcgtgg tttcacccgt
      781 tctgtgctct ccatgctgac agagattttt ctgaagcagg cgatggtggg gatagtcggg
      841 agtatcggca gcgccattgg cggtgctttc ggtggtggtg catctgcttc ctcggggacg
      901 gccatt
//
LOCUS       DAZFAB010000349          899 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZFAB010000349 DAZFAB010000000
VERSION     DAZFAB010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.354"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>899
                     /locus_tag="ACHJWA_005157"
     CDS             <1..>899
                     /locus_tag="ACHJWA_005157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO4311550.1"
                     /translation="DALAQKIREGWQPYGGPFSSYTDDGAALIQAIVAEGDVSTPVVV
                     KPTGGEGAVISATSDPGYYFVVVLAGQSNGMSYGEGLPLPETYDRPDPRIKQLARRST
                     VTPGGVACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGTVGQGLHIAKKLLPFI
                     PANAGILLVPCCRGGSAFTTGADGTYSDASGASENSTRWGVDKPLYKDLIGRTKAALK
                     KNPKNVLFAVVWMQGEFDFGGTPANHAAQFGALVDKFRADLADMAGQCVGGSAGGVPW
                     ICGDTTYFWKQKNESTYQTVYGS"
BASE COUNT          204 a          208 c          288 g          199 t
ORIGIN      
        1 gatgcacttg cgcaaaaaat tcgtgaagga tggcaaccat atggtgggcc gttttcttcg
       61 tatacggatg atggcgcagc acttattcag gcgattgtcg cagaaggtga tgtgagcaca
      121 cctgttgtgg tgaagccgac aggtggagaa ggtgcagtaa tcagcgccac cagcgacccc
      181 gggtattact ttgttgtggt tctggcaggg cagtcaaacg gcatgtcgta tggtgaaggt
      241 cttccgctgc cggagacata tgaccgtccg gacccgcgca ttaagcagct ggcgcgtcgc
      301 agtacggtga caccgggcgg tgtcgcctgt aaatataacg acatcattcc ggcggaccat
      361 tgtctgcatg atgtgcagga catgagccgc cttaaccatc cgaaagcgga cctgtcaaag
      421 gggcagtacg gaaccgtggg gcaggggctg catatcgcca aaaaattgct gccgtttata
      481 ccggcgaatg cgggcattct gctggttccg tgctgtcgtg gtggttcagc gttcaccacc
      541 ggagctgatg gcacatacag tgacgcgagt ggtgcctcgg agaattcaac ccgctggggt
      601 gtggacaagc cgctgtataa ggaccttatc ggtcgaacaa aagcagcact gaagaagaat
      661 ccgaaaaatg tgctgtttgc cgtggtgtgg atgcaggggg aatttgattt tggcggtacg
      721 ccggcaaatc acgcagcaca gtttggtgcg ctggttgata aattccgtgc agacctggcg
      781 gatatggcag gtcagtgcgt cggtggctct gctggcggtg ttccctggat atgtggagat
      841 acgacgtatt tctggaagca gaagaacgaa tccacgtacc agacggtgta cggcagcta
//
LOCUS       DAZFAB010000350          898 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZFAB010000350 DAZFAB010000000
VERSION     DAZFAB010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..898
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.355"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..188
                     /locus_tag="ACHJWA_005158"
     CDS             <1..188
                     /locus_tag="ACHJWA_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO4311551.1"
                     /translation="NIRFPNQMIEQINIALDQKGSGNFSAWVIEACRRRLINEKHAQF
                     VPNKDKHDQSTCSDRFT"
     gene            complement(270..584)
                     /locus_tag="ACHJWA_005159"
     CDS             complement(270..584)
                     /locus_tag="ACHJWA_005159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO4311552.1"
                     /translation="MLHDHVAECLEKKGLYRRAAERWAKVMVQLSDDQKRKVAAQKRA
                     ECLRKARRTPVSPVNLTEIKQAVNRLHSELGMGFEERRVFRRYKGTGEQNTSGNARSK
                     KC"
BASE COUNT          274 a          223 c          135 g          266 t
ORIGIN      
        1 gcaacatccg ctttcctaac caaatgattg aacaaattaa catcgctctt gatcagaaag
       61 gttcaggtaa tttttcagcg tgggttattg aagcctgcag aagaagatta attaatgaaa
      121 aacatgctca atttgtaccc aacaaagaca aacacgacca gagcacctgt tcagacaggt
      181 ttacttaaac gacttatata tgacacaaaa agcgaccact aaagtcgctt tttcttatgg
      241 taacaggcaa taacgctctc agatattttt tagcattttt ttgaccgcgc gtttccggac
      301 gtattctgtt ctcctgtccc tttatatcgt cggaataccc gccgctcttc aaatcccatt
      361 cccaactcag aatgtagtct gttgaccgct tgttttattt cggtcaggtt caccggtgaa
      421 accggagtcc ggcgcgcctt acgcaaacac tctgctcgtt tctgtgccgc cacttttctt
      481 ttctggtcat cacttagctg taccatcact tttgcccatc gttcagctgc tctccggtac
      541 agtccttttt tctccagaca ttctgccacg tgatcatgta gcataagtga cctccgatta
      601 tctacagact gccatcctga atttaccttc ccttaatgaa ataacaataa aaaacaaacc
      661 acgcaaaaac aataaaacaa cacacaaaaa aaactaaata ataaacaaaa ataatcacct
      721 tattttatta ttttttgagg gagcaattac tgaacaaaaa acgctgacta tatactcaaa
      781 accaaacaac tattctgcca atcaggtatc atggcaacac acggaattac cgtgtttttg
      841 ccttctctgc ccatacaata cgggcatata cttcattctc tattgtaata tttctatc
//
LOCUS       DAZFAB010000351          891 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZFAB010000351 DAZFAB010000000
VERSION     DAZFAB010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..891
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.356"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>891
                     /locus_tag="ACHJWA_005160"
     CDS             <1..>891
                     /locus_tag="ACHJWA_005160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO4311553.1"
                     /translation="SASFVPVETESDPMNVTFDNLAPEVQNAVMVKFDTCENITVDMV
                     ISAQELLQEDMATFDGHIVEALMKMPEVNAMYPELKLHAIGWVKHKCKPGAKWPEIQA
                     EMRIWKKRREGERKEAGKYTSVVDLARARANQQHTENSTGKINPVIAAIHREYKQTWK
                     TLDDELAYALWPGDVDAGNIDGSIHRWAKNEVIDNGREDWKRISASMRKQPDALRYDR
                     QTIFGLVRERPIDIHKDPVALNKYITEYLTTKGVFEDEGTNQSATDTLSSPVPETDAV
                     ETAIPDNEKTECKVEVEPSV"
BASE COUNT          271 a          216 c          220 g          184 t
ORIGIN      
        1 gtctgcttcc tttgtcccgg tcgagactga atccgatccg atgaacgtca cttttgacaa
       61 cctggcccct gaagtacaga acgctgtcat ggttaagttc gacacatgtg aaaacatcac
      121 tgttgatatg gtgattagcg cacaggaatt gttgcaggaa gacatggcaa cattcgacgg
      181 ccatatcgtt gaagcgttga tgaaaatgcc agaagttaac gctatgtatc cggagcttaa
      241 gctgcatgcc atcgggtggg ttaagcataa atgtaagcct ggtgccaaat ggcccgaaat
      301 tcaggcagag atgcgcatct ggaaaaaacg tcgcgaaggt gaacgcaagg aagccggaaa
      361 atacacgtct gttgttgatc tcgcccgcgc cagagccaat caacagcaca ctgaaaattc
      421 aacaggaaaa atcaacccgg tcattgctgc cattcatcgc gaatacaagc agacatggaa
      481 aacactggat gacgaactgg cctacgctct ctggcctggt gatgtggatg ccggaaacat
      541 tgacggcagc atccatcgct gggcaaaaaa tgaagttatc gacaacggcc gcgaagactg
      601 gaagcgtatc tcggcatcaa tgcgcaaaca gcctgatgcc cttcgctacg accgccagac
      661 tatttttggc cttgtccgtg aacgtccgat cgacattcac aaagaccctg tggcactgaa
      721 caaatacatt actgaatacc tgactacaaa gggcgtgttt gaagatgaag gaacaaatca
      781 gagcgcaact gatactctct cgtcgccagt accagaaact gatgcagtgg aaacggcaat
      841 tccagacaac gaaaaaaccg aatgcaaagt ggaagtcgaa ccatctgtag a
//
LOCUS       DAZFAB010000352          890 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZFAB010000352 DAZFAB010000000
VERSION     DAZFAB010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.357"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            30..299
                     /locus_tag="ACHJWA_005161"
     CDS             30..299
                     /locus_tag="ACHJWA_005161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO4311554.1"
                     /translation="MNILKKIMQRLCGCGKHDDCEHGQSLTVQLRLGPADILESDENG
                     IIPEQVRVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
     gene            301..378
                     /locus_tag="ACHJWA_005162"
                     /pseudo
     CDS             301..378
                     /locus_tag="ACHJWA_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309586.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
     gene            411..>890
                     /locus_tag="ACHJWA_005163"
     CDS             411..>890
                     /locus_tag="ACHJWA_005163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFO4311555.1"
                     /translation="MPGLISYVSSASFVNEMMELRQQVMEGQIGGFLLGGERVRVSYM
                     PDTGRFLAESEGQGRVYAELLNIAFNDGVNVLRNRILSALPGMGGRNSLQEKISECAF
                     TVDIEKLQCPGDALQCPITLEQPEKGVFVKNSDGSDVCTLFDAAAFSRLTGEDLPHPL
                     "
BASE COUNT          243 a          152 c          259 g          236 t
ORIGIN      
        1 cagtgataca ggcagaacag gagaatgaca tgaacatatt aaaaaaaatt atgcagcgtc
       61 tgtgcggttg cggaaagcat gatgactgtg aacacgggca gtcgcttaca gtacaactgc
      121 gactggggcc ggcagacatc ctggagtcag atgagaatgg cattatcccg gagcaggtca
      181 gggtaatcac gcaggtggtg atactggatg cggataaaaa gcagatacag tgtgtggtaa
      241 gaccgctgca aatcctgcgt gctgacggga cgtgggaaaa tattggcggg atgaagtaac
      301 ccgacagctt cacaaaaccg gagtccggct ccggtttttg ttgtcatgtc cggtggatgt
      361 ttgttaatga agacttgagg gaatatttat ccgtatgaag gagcatggta atgcctggat
      421 taatatcata tgtgtcatcg gcttcattcg tgaatgagat gatggagctg cgtcagcagg
      481 taatggaggg gcagattggt ggatttctcc tgggagggga gagggttaga gtttcttata
      541 tgccagatac aggccgtttt ttagcagaaa gtgaagggca gggacgggtt tatgcagaat
      601 tattgaatat tgcttttaat gatggagtta atgtgctcag aaacaggata ttaagtgcgc
      661 tccctggaat gggagggcga aactctttgc aggaaaaaat atcggagtgt gcctttactg
      721 tcgatattga aaaacttcaa tgtcctggtg atgcgcttca atgtccaatt acactggagc
      781 agcctgaaaa aggtgttttt gtgaagaatt cagatggttc agatgtatgt actttatttg
      841 atgccgctgc attttctcgt ttgactggtg aagacttacc ccacccactg
//
LOCUS       DAZFAB010000353          890 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZFAB010000353 DAZFAB010000000
VERSION     DAZFAB010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.358"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            45..845
                     /locus_tag="ACHJWA_005164"
     CDS             45..845
                     /locus_tag="ACHJWA_005164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311556.1"
                     /translation="MNSKKLCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVARAENMNWMQALRFVAAKGHQKAIIV
                     YQDMLQTGKYDSALKSTVWSDYKNEKLTDAISLRYLVRFTLVDVATGEWATWSPVNYE
                     SRVIFPPAGITKTSNKELSNDHVTEAQLFNLKQKTYVSMVKDLVSRFQ"
BASE COUNT          283 a          176 c          202 g          229 t
ORIGIN      
        1 gctaaatatt ctaatcatta tgacaggcga gggagtgtcc aattatgaat tcaaaaaagc
       61 tttgttgcat atgtgtgtta ttctcgctgc ttgcaggatg tgcctctgaa tcttctattg
      121 atgaaaagaa gaaaaaagct caagtcacac aaagtaatat taataaaaac actccccagc
      181 aactgacaga caaagattta ttcggtaatg aaaccactct ggccgtatcc gaagaggata
      241 ttcaagcagc gcttgatgga gatgagttcc gcgttcccct taattctcct gtaatacttg
      301 ttcaatccgg caaccgcgca ccggaaacca ttatgcagga ggagatgcga aaatattata
      361 ctgtttccac attctctggt atcccggaca ggcaaaagcc tctgacttgt aacaaaaaca
      421 aagataaaaa tgagaacgag gatgttgccc gtgctgagaa tatgaactgg atgcaggcac
      481 tgcgttttgt ggctgcaaaa ggacatcaga aagcgattat tgtttaccag gatatgttgc
      541 agacaggaaa atatgactct gcgctgaaat caacagtatg gtccgactat aaaaatgaaa
      601 aactcactga cgctatctcc ctgcgctacc tggtacgttt cacgctggtg gatgtggcaa
      661 caggtgagtg ggctacctgg tcgccggtga attatgaatc gagagtaatt tttccgccgg
      721 ctggaataac aaaaacgagc aataaagaac tatcaaatga ccatgttacg gaagcgcaac
      781 tgtttaattt gaaacaaaaa acatatgtat cgatggtgaa agacttggtg agtcgattcc
      841 agtaatcgtt attgttatgc tgccgcctgt agtttagggt ggcagcaaac
//
LOCUS       DAZFAB010000354          875 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZFAB010000354 DAZFAB010000000
VERSION     DAZFAB010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..875
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.359"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>875
                     /gene="espR4"
                     /locus_tag="ACHJWA_005165"
     CDS             <1..>875
                     /gene="espR4"
                     /locus_tag="ACHJWA_005165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector leucine-rich repeat protein EspR4"
                     /protein_id="HFO4311557.1"
                     /translation="NMLSEFKLLREQCFRWGNYTLLFENYEAYDKTGSITIEKNQGEG
                     TLPIRHKLEFISTNIAELLDKLTKITDARLCKGFSDWASSVKEGGSNDLKENVDRALV
                     RMFKCVKLHSNELDLSYLFLGSVPPLPDWIEMLSLVYNELDSIQVPESCKELELDFNN
                     LTEFPQVPDGITLISVNNNLISHIDSFPPKAKKIFISHNKLSETPAIPDTAKVFDCGY
                     NKIQEIRYFPKNLKEARIGYNNIEVVPAIPGNLKILFMECNPIKEAFLMPWTLTGICY
                     EISQRKYIVTNPADY"
BASE COUNT          289 a          146 c          178 g          262 t
ORIGIN      
        1 gaatatgctg tctgaattta agttattgag agagcaatgt ttcaggtggg gtaattatac
       61 tctattgttt gaaaattatg aggcttatga taagacgggg tctatcacga tagaaaaaaa
      121 tcagggggag gggactttac ccattcggca taaattagag tttatatcga ctaatattgc
      181 agagttgctg gacaagttaa cgaaaattac agatgccaga ctttgcaaag gcttcagtga
      241 ctgggctagt tcagtcaaag aaggcggatc gaatgacctc aaagaaaatg tagatagagc
      301 gttggtgaga atgtttaaat gtgttaagct tcacagtaat gaacttgact tatcatacct
      361 atttttgggt tctgtgccgc ctcttccaga ttggattgaa atgcttagcc ttgtttataa
      421 tgaacttgat tcaatacagg ttcccgaatc atgcaaagaa ttagaactcg atttcaataa
      481 ccttacagaa tttccacaag tacctgatgg aattaccctg atctccgtaa ataataacct
      541 gatatcgcat attgactcat ttccgccaaa ggctaagaaa atttttatta gtcacaataa
      601 gctatcggaa acaccagcaa taccagacac cgctaaggtt tttgattgtg gctataataa
      661 gatacaagaa attcgatact tccccaaaaa tctgaaagaa gcgcgtattg gatataataa
      721 tattgaggtt gttcctgcga tacctggcaa tttaaaaata ctttttatgg aatgtaatcc
      781 tattaaagag gcatttttaa tgccatggac tctgacaggg atctgctatg aaatatcgca
      841 gcgaaaatat attgttacga atccagccga ttatg
//
LOCUS       DAZFAB010000355          872 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZFAB010000355 DAZFAB010000000
VERSION     DAZFAB010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..872
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.360"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(57..323)
                     /locus_tag="ACHJWA_005166"
     CDS             complement(57..323)
                     /locus_tag="ACHJWA_005166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HFO4311558.1"
                     /translation="MIITIALISLLSACTRENSLKENQNKRSGESQAIVDARQFFSEH
                     PEYISSAELEEKLYREFAQVTTVPAYKEMSMYQLLVISQDRLTH"
     gene            complement(353..853)
                     /locus_tag="ACHJWA_005167"
     CDS             complement(353..853)
                     /locus_tag="ACHJWA_005167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4311559.1"
                     /translation="MQVFSSDVYFTVGTNALLASQKEYYSDLVALVDLGHSFVVIDEH
                     QHRNLKPNTEPVNILLSNNFIWINKNITLSDLTHFLVSNLHTQNVYSTQEPLTHDEID
                     ILRLCVSYSLKQIAIIKGIDYKTVSYHKIRALNQLNIKGTVELFIALCEWDKHYFKLQ
                     SCVRES"
BASE COUNT          271 a          147 c          152 g          302 t
ORIGIN      
        1 gtatcgtagt caaaaattta taagaatcta cacgcaaaaa acgtgtagat ttttatttaa
       61 tgagttaaac gatcttgcga aatgacaagc aattgataca tactcatctc tttataagct
      121 ggaactgtag taacctgtgc aaattcgcga tatagctttt cctccagttc tgctgatgat
      181 atatattccg ggtgttcgct aaagaactgt cttgcatcta ctatcgcttg ggattcgcca
      241 cttcttttat tttgattctc ctttaatgaa ttttctctgg tgcatgcaga gagcaacgat
      301 atcagcgcga tggttattat gattttacta ttatatcatt actaatgaca tatcaacttt
      361 ctcttacgca agattggagt ttaaaataat gtttatccca ttcacagagt gctataaaca
      421 actcaacagt accttttatg ttaagttgat tgagggctct tattttgtga tatgatactg
      481 ttttataatc aattccttta ataatggcaa tttgctttaa tgaatatgaa acacataacc
      541 gcaggatatc tatctcatca tgggtaagtg gttcttgtgt ggaatacaca ttttgtgtat
      601 gaagatttga aaccaggaaa tgcgttaaat cagataatgt aatattctta ttgatccaga
      661 tgaaattgtt actcaaaagg atgtttacag gttctgtatt tggtttgaga tttcgatgtt
      721 gatgctcatc aataacaacg aacgaatgtc caagatcaac caacgcaact aaatcgctgt
      781 aatattcctt ttgggatgct aataatgcat tagtgcctac agtgaagtag acatcactgg
      841 aaaagacttg catatgccaa cctgtactct aa
//
LOCUS       DAZFAB010000356          849 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZFAB010000356 DAZFAB010000000
VERSION     DAZFAB010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..849
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.361"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..260
                     /locus_tag="ACHJWA_005168"
     CDS             <1..260
                     /locus_tag="ACHJWA_005168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO4311560.1"
                     /translation="KKLMQRLCGCGKHDDREHGELLTAQLRLGPADILESDENGIIPE
                     QDRVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
     gene            374..>849
                     /locus_tag="ACHJWA_005169"
     CDS             374..>849
                     /locus_tag="ACHJWA_005169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFO4311561.1"
                     /translation="MPLTSDIRSHSFNLGVEVVRARIVANGRGDITVGGETVSIVYDS
                     TNGRFSSSGGNGGLLSELLLLGFNSGPRALGERMLSMLSDSGEAQSQESIQNKISQCK
                     FSVCPERLQCPLEAIQCPITLEQPEKGIFVKNSDGSDVCTLFDAAAFSRLVGEGLP"
BASE COUNT          217 a          151 c          237 g          244 t
ORIGIN      
        1 taaaaaaact tatgcagcgt ctgtgtggtt gcggaaagca tgatgaccgt gaacacgggg
       61 agttacttac agcacagctg cgactggggc cggcagacat cctggagtca gatgagaatg
      121 gtattatccc ggagcaggac agggtaatca cgcaggtggt gatactggat gcggataaaa
      181 agcagataca gtgtgtggta agaccgctgc aaatcctgcg tgctgacggg acgtgggaaa
      241 atattggcgg gatgaaatag ccgacagctt cacaaaaacc ggagtccggc tccggttttt
      301 tgttgtcacg tatgggggat gtttgttaca gctatttaag tctggagttc aaattaaaat
      361 agggagcttt gttatgccat taacctcaga tattagatca cattcattta atcttggggt
      421 ggaggttgtt cgtgcccgaa ttgtagccaa tgggcgcgga gatattacag tcggtggtga
      481 aactgtcagt attgtgtatg attctactaa tgggcgcttt tcatccagtg gcggtaatgg
      541 cggattgctt tctgagttat tgcttttggg atttaatagt ggtcctcgag cccttggtga
      601 gagaatgcta agtatgcttt cggactcagg tgaagcacaa tcgcaagaga gtattcagaa
      661 caaaatatct caatgtaagt tttctgtttg tccagagaga cttcagtgcc cgcttgaggc
      721 tattcagtgt ccaattacac tggagcagcc tgaaaaaggt atttttgtga agaattcaga
      781 tggttcagat gtatgtactt tatttgatgc cgctgcattt tctcgtttgg ttggtgaagg
      841 cttacccca
//
LOCUS       DAZFAB010000357          823 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZFAB010000357 DAZFAB010000000
VERSION     DAZFAB010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.362"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..384
                     /locus_tag="ACHJWA_005170"
     CDS             <1..384
                     /locus_tag="ACHJWA_005170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO4311562.1"
                     /translation="SSLPQGVALTGDGGNNLLAAFYIQTDAKDLNVMYHNAKVATNNL
                     KLGTFGAFDNEWHTLAFRFAGNNSLQVTPVIDGQDGTPFTLTQSPVSAFAADKLHVTD
                     ITRNATYPVLIDSIAVEVNNADAAA"
     gene            521..700
                     /locus_tag="ACHJWA_005171"
     CDS             521..700
                     /locus_tag="ACHJWA_005171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO4311563.1"
                     /translation="MTFLNQLMLYFCTVVCVLYLLSGGYRAMRDFWRRQIDKRAAEKI
                     SASQSAGSKPEEPLI"
     gene            741..>823
                     /locus_tag="ACHJWA_005172"
     CDS             741..>823
                     /locus_tag="ACHJWA_005172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO4311564.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALK"
BASE COUNT          224 a          197 c          217 g          185 t
ORIGIN      
        1 tcttcactgc cacagggcgt tgccctgacc ggagacgggg ggaataacct gctggcagcg
       61 ttctacatcc agacagatgc aaaagacctg aatgtgatgt accacaatgc gaaagtggcg
      121 acaaacaacc tgaaactggg aacctttggc gcatttgata acgaatggca tacgctggct
      181 ttccgctttg ccgggaataa cagccttcag gtgacgccgg ttattgatgg tcaggatggc
      241 acaccgttca cgctgacgca gtcaccggtc agtgcatttg cggcggataa actgcatgtg
      301 acagacatta ccaggaatgc gacttacccg gtgctgattg acagcattgc ggtggaagtg
      361 aacaacgcgg atgccgcggc atgataaaaa aagccgccag ttgcctgaga cctctggcgg
      421 tgtgagattc atggagaatc aaggaaagat actaccactt tcgtcatcgg catttttaaa
      481 tgaaaactgt ttacttagtc aaccataacg gtaagaaatt atgacatttc tgaaccagtt
      541 aatgctgtac ttctgtacgg tggtctgtgt gctgtatctc ctttcgggtg ggtacagggc
      601 catgcgtgac ttctggcgca gacagattga caaaagggcc gctgagaaaa tcagcgccag
      661 tcagtcagcc ggaagcaaac ccgaagagcc gctcatttag cggcaacttt cttaatcaca
      721 tctttcgacg agaaaatccc atgtcagaaa ttacatccct ggtcactgct gaagcagtga
      781 aggaagtcct gcgctctgaa gaagtccgga gcgcactgaa aca
//
LOCUS       DAZFAB010000358          822 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZFAB010000358 DAZFAB010000000
VERSION     DAZFAB010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.363"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            209..284
                     /locus_tag="ACHJWA_005173"
     tRNA            209..284
                     /locus_tag="ACHJWA_005173"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:242..244,aa:Ile,seq:cat)
BASE COUNT          239 a          150 c          186 g          247 t
ORIGIN      
        1 gaatcgatga taactataga ttgtgctaat tattaacatt tgcttgttga ctgcgagtat
       61 cgactttgcg gaatggaggt ggtgtcgggt tttatgtcgc atgtattgac gacccaggat
      121 ggatgagaac gcataaaaat ctgtgattta catcataaga tcatcatgtt tattgcgtgg
      181 gttataagta ttgttattgc atacttaagg ccctttagct cagtggttag agcaggcgac
      241 tcataatcgt ccggtcgctg gttcaagtcc agcaagggcc accaaatcat cattcatgta
      301 tcaggaagag ttaagggtac aagggttact cgatggcggt ctatatttca cgattaacgc
      361 catttcaagg aagaagtgcg gtgggtggtt tcgaatgagg gctggtgttt tggtacgtgg
      421 cacgccactg caatgtattt atctatcagt actcattcaa aagtatttgg aaaaaatgcc
      481 tgctatgcca agcgggcaaa ttgcttacat gtggaattaa gtaattaacg ataaagccag
      541 cgtattatca tttattatac ccattcattt aatcaggtta attactcaaa tcactgaact
      601 attctctgtt cgctcactga tgataaggga gtaccacaac caatgtatgt agaacaatgc
      661 cgtagagtaa ggatgaaatg tctctgccac gccttaaaca tctactactg actagaacac
      721 ccgccgcagg tagatgagtt ttgtgagcgt tacaggagcg tggctatgct cattaatcaa
      781 cgctatttag atataaattt ctaacactcg aaaaacgatt tt
//
LOCUS       DAZFAB010000359          811 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZFAB010000359 DAZFAB010000000
VERSION     DAZFAB010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.364"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            113..>811
                     /locus_tag="ACHJWA_005174"
     CDS             113..>811
                     /locus_tag="ACHJWA_005174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO4311565.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGISEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYH"
BASE COUNT          268 a          137 c          171 g          235 t
ORIGIN      
        1 atatttttat ttctaataga cgagattttt cctgttatga tataatatgc cgaattaaca
       61 catgttaacg atttaccagt aatgtaaata aattttcgag gagatcattc ctgtgggacg
      121 taaatgggcc aatattgttg ctaaaaaaac ggctaaagac ggtgcaacgt ctaaaattta
      181 tgcaaaattc ggtgtagaaa tctatgctgc cgctaaacaa ggtgaacccg atccagaatt
      241 aaacacatct ttaaaattcg ttattgaacg tgcaaagcag gcacaagttc caaagcacgt
      301 tattgataaa gcaattgata aagccaaagg cggcggagat gaaacattcg tgcagggccg
      361 ttatgaaggc tttggtccta atggctcaat gattatcgcc gagacattga cttcaaatgt
      421 taaccgtacg attgctaacg ttcgcacaat tttcaataaa aaaggcggca atatcggagc
      481 ggcaggttct gtcagctata tgtttgacaa tactggtgtg attgtattta aaggatcaga
      541 ccctgaccat atttttgaaa ttttacttga agctgaagtt gatgttcgtg atgtgactga
      601 agaagaaggt aacattgtta tttataccga acctactgac cttcataaag gaatcgcggc
      661 tctaaaagca gctggaatca gtgagttctc aacaacagaa ttagaaatga ttgctcaatc
      721 tgaagttgag ctttcaccag aagatttaga aatctttgaa gggcttgttg atgcccttga
      781 agatgacgac gatgtacaaa aagtttatca t
//
LOCUS       DAZFAB010000360          806 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZFAB010000360 DAZFAB010000000
VERSION     DAZFAB010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.365"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            85..654
                     /locus_tag="ACHJWA_005175"
     CDS             85..654
                     /locus_tag="ACHJWA_005175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HFO4311566.1"
                     /translation="MNMMAVPFHGNSLYVVNHNGEPYVPMKPVVAGMGLAWQSQLAKL
                     RQRFASTITEIVMVAEDGKQRNMVSMPLRKLAGWLQTINPNKVKPEIRDKVIRYQEEC
                     DDVLYEYWTKGFVVNPRKMSVMEELNQACADMKRDKNIASVFATGLNEWKQVKAAHVS
                     KIRTLVNEANMLIDFVLADTGKGKITKAD"
     gene            654..800
                     /locus_tag="ACHJWA_005176"
     CDS             654..800
                     /locus_tag="ACHJWA_005176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311567.1"
                     /translation="MGWLMISDKLITLVKSLCVLVGISFLVMLVAIFFSTAWRVLTLS
                     GLVG"
BASE COUNT          219 a          161 c          217 g          209 t
ORIGIN      
        1 ttgaggatac cggtttggct gtcggtgcct ttctgtgggc tggattcctg tgagacaagg
       61 ttcatcacta aaaggaaata accgatgaat atgatggccg tgccgtttca cggcaactct
      121 ctttatgtag ttaaccataa tggcgaacca tacgttccca tgaaacctgt cgttgcgggg
      181 atggggctgg cctggcaatc acagttggct aagttaagac agcgttttgc gtcaactata
      241 acggaaatcg ttatggttgc tgaggatggg aaacaacgca atatggtgtc catgccactt
      301 cgaaaacttg ccggctggct acaaaccatt aatcccaaca aagtaaaacc cgaaatccgc
      361 gataaggtca tccggtatca ggaagagtgc gacgatgttc tttacgagta ctggacgaag
      421 ggttttgtcg ttaatccccg taaaatgagc gtgatggaag aactcaacca ggcttgtgct
      481 gacatgaaac gggataaaaa cattgccagt gtgtttgcta ccgggctgaa tgagtggaaa
      541 caggttaaag ccgcgcatgt atcaaaaatc cgtacgctgg taaatgaagc gaatatgctg
      601 attgattttg tcctggctga tacaggcaaa gggaaaataa caaaggcgga ttgatggggt
      661 ggctaatgat atcagataaa ctcataacgc tggtgaagag cctctgtgta cttgtcggca
      721 tttcattttt agtcatgctg gttgccattt tcttttccac cgcctggcga gtcctgacgt
      781 tatcgggact ggtggggtga aagaga
//
LOCUS       DAZFAB010000361          803 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZFAB010000361 DAZFAB010000000
VERSION     DAZFAB010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.366"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..>803)
                     /gene="espX1"
                     /locus_tag="ACHJWA_005177"
     CDS             complement(<1..>803)
                     /gene="espX1"
                     /locus_tag="ACHJWA_005177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HFO4311568.1"
                     /translation="LVKVFCEYKTEFLILFDDFFRSQDLPKPSPVLHHFFQYTHLRDA
                     HFYRCKLIEHTVQFSFFKHKGITLLRLDVFDDRTSECLSEEIKIYQECHEKFIKFLKA
                     NFNQEIYPELYTPEIFYEACRNLQSFYDHQETSQNAKYSAIVKKKSYFNKEIRNLIKK
                     NIYPELYNEQCNKIPASSTDDNQKITWQNFKTSNAAYSQLCEKLSLLKSSPSRLIEKS
                     AYCSNENMITDKFDVVFSYCGDNVKEFILLLPYNKSLEMHELNEQNIQY"
BASE COUNT          245 a          124 c          135 g          299 t
ORIGIN      
        1 aatactggat gttttgctcg ttaagttcat gcatctccag cgatttatta tagggtaata
       61 aaagtataaa ttcctttaca ttatctccgc agtaagaaaa gacaacatca aatttatcgg
      121 tgatcatgtt ttcatttgag cagtaagctg atttctctat taaccgactg ggcgagcttt
      181 ttaataaaga taatttttca catagttgtg aatatgccgc attgctagtt ttaaaattct
      241 gccatgttat tttctggtta tcatctgtcg atgaggcggg gattttattg cattgctcat
      301 tgtatagctc aggataaata ttctttttta ttagatttct gatttcctta ttaaaataac
      361 tttttttctt gactattgca ctgtacttag cgttttgtga cgtctcctga tgatcataaa
      421 atgactggag atttctgcag gcctcataaa atatttcagg agtgtatagc tctgggtata
      481 tttcctgatt gaagtttgcc tttaaaaatt taataaattt ttcatggcat tcttgatata
      541 tcttaatctc ttcagaaaga cattcgcttg ttctgtcatc aaaaacatcc aatctaagta
      601 atgtgatgcc tttgtgttta aaaaaagaaa attgaactgt atgttctatg agtttacatc
      661 tataaaaatg tgcatctctc agatgagtat attgaaaaaa atgatggagg actggagatg
      721 gtttaggcaa atcctggctt ctgaaaaagt catcaaacaa aattaagaac tctgttttat
      781 actcgcaaaa cacttttact aac
//
LOCUS       DAZFAB010000362          802 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZFAB010000362 DAZFAB010000000
VERSION     DAZFAB010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.367"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..509)
                     /locus_tag="ACHJWA_005178"
     CDS             complement(<1..509)
                     /locus_tag="ACHJWA_005178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO4311569.1"
                     /translation="MLYSNILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPEDY
                     FRMSPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALSWWLVCRGEIHKFR
                     CVPHLTGRRFEHGVTDCYTLFRDAYHLAGIDMPDFEREDDWWRNGQNLYLDNMAVTGF
                     YRVPLSSAQA"
     gene            complement(563..>802)
                     /locus_tag="ACHJWA_005179"
     CDS             complement(563..>802)
                     /locus_tag="ACHJWA_005179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22AR C-terminal domain-containing protein"
                     /protein_id="HFO4311570.1"
                     /translation="NRSQALFRELYPALKQIQSNYSGRCYDYGHEFSYVIGMARDVLI
                     NHTRDVDINEPDGPTNLSAWMRLKNKELPPSPHHY"
BASE COUNT          185 a          231 c          199 g          187 t
ORIGIN      
        1 gcctgtgcag aggacagggg cacccggtaa aagccggtga ccgccatatt gtccaggtaa
       61 aggttctgac cgttgcgcca ccagtcatcc tcacgctcaa aatccggcat atcaattccc
      121 gccagatggt aggcatcccg gaacagcgtg taacagtccg tcaccccgtg ctcaaagcgc
      181 cgtcctgtca gatgtggcac acagcggaat ttatgaattt ccccccggca gaccagccac
      241 caggacagtg cactttttat ctgcagccgc cggtcggcct cgctcagcca gggcagacca
      301 ccggggtggc tgtggaccag tgccacaatc tccccctgca tttctgcccg cagccagtcc
      361 tccggcgaca tccggaaata atcttccggc tcaccggaga tattcacgca gggaaaatat
      421 ctttcccctt ccggcgttct caccacgaag ccgcacgact ccgctggtgc acatcgccgg
      481 gcgtgcgcca atatattgct atagagcatg agaactcctg ataaaaaccc agccgaagct
      541 gggtcatttc gttggcaatc tgttagtagt gatgcggtga aggaggtaat tctttattct
      601 taagtctcat ccatgcggaa agattcgttg gtccgtctgg ctcattgata tcaacatctc
      661 gtgtgtgatt aattaaaacg tctctcgcca ttccaataac atacgagaac tcatgaccgt
      721 agtcgtagca tctgccggaa tagttcgatt gaatttgttt tagcgccgga tacagttcgc
      781 ggaataatgc ctgtgagcgg tt
//
LOCUS       DAZFAB010000363          794 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZFAB010000363 DAZFAB010000000
VERSION     DAZFAB010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..794
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.368"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..143)
                     /locus_tag="ACHJWA_005180"
     CDS             complement(<1..143)
                     /locus_tag="ACHJWA_005180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO4311571.1"
                     /translation="MFPATKGALMRIYRRKCKCCNEWFIPKYQNQYWCNEICGTKIAL
                     ERR"
     gene            complement(91..657)
                     /locus_tag="ACHJWA_005181"
     CDS             complement(91..657)
                     /locus_tag="ACHJWA_005181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO4311572.1"
                     /translation="MAKPARRRCNRKREDLTVKRIFELLSFDKSTGVFRWKVPTQGRI
                     ALNSVAGAFDSNGYSMIMIDGRRYKTHVLVFYITHNRWPAGQIDHVNGIRTDNRPENL
                     RECLPIENSRNIRIRKNSKSGCRGVTWHKRQKKWNVRLGFHGKSKHFGCFDDLELAVL
                     VAEEARDKYYGDFSGNERSTYANLSKEM"
BASE COUNT          213 a          188 c          130 g          263 t
ORIGIN      
        1 cttcgtcgtt cgagtgctat cttggttcca caaatctcat tacaccaata ttgattttga
       61 tattttggta taaaccattc attgcaacat ttacatttcc ttcgatagat tcgcataagt
      121 gctcctttcg ttgccggaaa aatcaccgta atacttatct cgggcttctt cagcaactag
      181 taccgctaac tccagatcat caaagcatcc gaagtgttta ctcttgccat ggaatcctag
      241 cctaacattc cattttttct gtcgtttgtg ccaagtaacc cctctgcaac ctgatttgct
      301 attctttcgg atccttatat ttcttgaatt ttctattggc aggcattctc ttaaattttc
      361 tggcctattg tcggtcctaa ttccattaac gtggtcaatt tgaccagcag gccaacgatt
      421 atgagttatg taaaaaacta agacgtgagt tttatatcta cgcccatcta tcatgatcat
      481 tgaataaccg ttggaatcaa aagctccagc aacactattt aatgctatcc ttccctgagt
      541 gggaactttc catctaaata ccccggtaga tttatcgaaa cttagtaact caaatatcct
      601 tttaacagtt aaatcttctc ttttacggtt acatcgtctt cgcgctggtt tagccatcgc
      661 cttcttcctc cgtaatggtt ttctgaattt ggccacctga acagagctca ccaaagctat
      721 ggatgtcggt atttccacaa taccaagatg gcgaaaataa ctgcatgata agcctcaggg
      781 aaaagggaag acac
//
LOCUS       DAZFAB010000364          775 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZFAB010000364 DAZFAB010000000
VERSION     DAZFAB010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.369"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            126..>775
                     /locus_tag="ACHJWA_005182"
     rRNA            126..>775
                     /locus_tag="ACHJWA_005182"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          214 a          168 c          230 g          163 t
ORIGIN      
        1 agacaatctg tgtgggcact cgaagatacg gattcttaac gtcgcaagac gaaaaatgaa
       61 taccaagtct caagagtgaa cacgtaattc attacgaagt ttaattcttt gagcatcaaa
      121 cttttaaatt gaagagtttg atcatggctc agattgaacg ctggcggcag gcctaacaca
      181 tgcaagtcga acggtaacag gaagaagctt gcttctttgc tgacgagtgg cggacgggtg
      241 agtaatgtct gggaaactgc ctgatggagg gggataacta ctggaaacgg tagctaatac
      301 cgcataacgt cgcaagacca aagaggggga ccttcgggcc tcttgccatc ggatgtgccc
      361 agatgggatt agctagtagg tggggtaacg gctcacctag gcgacgatcc ctagctggtc
      421 tgagaggatg accagccaca ctggaactga gacacggtcc agactcctac gggaggcagc
      481 agtggggaat attgcacaat gggcgcaagc ctgatgcagc catgccgcgt gtatgaagaa
      541 ggccttcggg ttgtaaagta ctttcagcgg ggaggaaggg agtaaagtta atacctttgc
      601 tcattgacgt tacccgcaga agaagcaccg gctaactccg tgccagcagc cgcggtaata
      661 cggagggtgc aagcgttaat cggaattact gggcgtaaag cgcacgcagg cggtttgtta
      721 agtcagatgt gaaatccccg ggctcaacct gggaactgca tctgatactg gcaag
//
LOCUS       DAZFAB010000365          775 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZFAB010000365 DAZFAB010000000
VERSION     DAZFAB010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.370"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            147..332
                     /locus_tag="ACHJWA_005183"
     CDS             147..332
                     /locus_tag="ACHJWA_005183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFO4311573.1"
                     /translation="MELLLSLRIRSLIFLQKLIGRNNLTQRTRIKRLARRIIYFSRTV
                     VRNKKVIGTFIKKHIFY"
     gene            573..>775
                     /locus_tag="ACHJWA_005184"
     CDS             573..>775
                     /locus_tag="ACHJWA_005184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-LEE encoded effector protein NleB"
                     /protein_id="HFO4311574.1"
                     /translation="MLSSLNVLQSSFRGKTALSNSTLLQKVSFAGKEYPLEPIDEKTP
                     ILFQWFEARPERYEKGEVPILNT"
BASE COUNT          257 a          138 c          141 g          239 t
ORIGIN      
        1 tgagtatcac aggcacatgt attttagccc cctgagtcta cagacaacca atattactta
       61 aataagtgat aatcttaatg ctgtttttta gaacagatga ttgatatttt tgggccggag
      121 aaacgcagac aggctttgtt gaataaatcg agcttttgct gagtttaagg atcagatcac
      181 tcattttcct gcaaaagctc attgggcgaa acaatctgac tcagagaacc cggattaaac
      241 gtctggcccg cagaatcatc tatttctcac gcactgtagt acgtaataaa aaagtcatcg
      301 gtacttttat caaaaaacat atattctatt aattattgcc atcacctttt tcttattgac
      361 aacattactc tgctgattgt taaaatctaa cctgtattca gggttacgat tctatacaat
      421 agaacaggga ggggaaagga tgtcatcgtt aatgagactt tttgttaatg tcaacaatgc
      481 tatgaattta ttttagaaaa acaattaagc tgacgtagtg ttccgtctaa atgacggggc
      541 gatattaaca tgattagaac aagaggaatt ttatgttatc ttcattaaat gtccttcaat
      601 ccagcttcag aggaaagaca gctttatcaa atagtacact tctccagaaa gtttcttttg
      661 ctggaaaaga atatcctctg gaacctattg atgaaaaaac ccctattctt tttcagtggt
      721 ttgaagcaag gccagagcga tacgaaaaag gagaagtacc aatattgaat accaa
//
LOCUS       DAZFAB010000366          772 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZFAB010000366 DAZFAB010000000
VERSION     DAZFAB010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.371"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(1..258)
                     /locus_tag="ACHJWA_005185"
                     /pseudo
     CDS             complement(1..258)
                     /locus_tag="ACHJWA_005185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323627.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-f3"
     gene            complement(260..>772)
                     /locus_tag="ACHJWA_005186"
     CDS             complement(260..>772)
                     /locus_tag="ACHJWA_005186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HFO4311575.1"
                     /translation="LKGGWNLYTYPLSPVNGMDPLGLYEFKSKNIDDIGIFALAMCNG
                     ESINENKEYGGLICKKQGEYFPMNPISSNDNDSVDLRNIKCPEGSERVGDYHTHGFYS
                     DDKGNKVTKENDVYDSLNFSSKDLTNSYMNGMGKKECSSYLGTPNNTYLKYNPKAKGN
                     GVTIIRQGSN"
BASE COUNT          226 a          160 c           95 g          291 t
ORIGIN      
        1 atgacattcc cccgcccccc attatgtcat tgtgggttga tgttgggctt tcataaaaaa
       61 atacatcttt tttgaactag cattaattat atttacttta tatattaaaa tattttctcc
      121 atctggaaat attttagcca cacaatataa agcagctaaa tccgaattac ttaaaaatat
      181 attgtcatca ctcataactc cagccattaa atgactggag actaaagcaa ggaaataaca
      241 acattgtatt gccttcattt taattactcc cttgcctgat aattgtaact ccattccctt
      301 tagctttggg attatatttt agataggtgt tatttggtgt tcccaagtaa ctactgcatt
      361 ctttttttcc cattccattc atataagaat tcgttaaatc tttgcttgaa aaatttagac
      421 tatcataaac atcattttct tttgttactt tatttccttt atcgtcagag taaaaaccgt
      481 gagtgtgata atcgcctact ctctctgaac cttcagggca ttttatattt cgcaagtcta
      541 cactatcatt atcatttgaa cttatcggat tcatggggaa atattcacct tgcttcttac
      601 atattagtcc accatattct ttattctcgt taattgattc tccattacac attgccaatg
      661 caaatattcc aatatcatct atattttttg atttaaattc atataatcct aatggatcca
      721 tgccattcac cgggctcagc ggatatgtat acaggttcca tccccccttc ag
//
LOCUS       DAZFAB010000367          751 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZFAB010000367 DAZFAB010000000
VERSION     DAZFAB010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.372"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(54..596)
                     /locus_tag="ACHJWA_005187"
     CDS             complement(54..596)
                     /locus_tag="ACHJWA_005187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4311576.1"
                     /translation="MKHSIIAVAVLSSVFMSAGIFAAEVDTGTLTIKGNIAESPCTFV
                     AGGDSVSINMPTVPTTVFDGKAKYSTYDGAVGVTSSMLKITCPKTIDGVKLSLITNEK
                     ILGNDKAISSSNDTVGYYLYLGDSSDVLDISAPFNIESYKTADGQYAIPFKAKYLKLT
                     DSGVASGTVNSSLVMRVAQD"
BASE COUNT          253 a          144 c          120 g          234 t
ORIGIN      
        1 aatatggcgg ccattttaac ttactgaaaa aacaagatga ttattttaaa tatttaatcc
       61 tgggcaacac gcataaccaa tgaagagtta actgtaccag atgcaacacc actgtcagtc
      121 aacttcagat attttgcctt aaacggtatt gcatattgtc catcagcagt tttataactt
      181 tcaatattaa atggtgcgga aatatccagt acgtcactgc tatctcccaa atagagatag
      241 tatccaactg tgtcattact actggatatt gccttatcat taccaagaat tttctcgttg
      301 gtgatcaacg aaagttttac gccatcaata gtcttcggac aagtaatttt cagcatactg
      361 ctggttacac caaccgcgcc atcataagtt gaatatttcg ctttaccatc aaatacagtg
      421 gtgggtacag tcggcatatt aatactgact gaatcaccgc cagcgacaaa tgtacatggt
      481 gattctgcaa tattaccttt aatggttaaa gttccggtat ctacttcagc agcgaaaata
      541 ccagcactca taaatacaga agataagaca gcgacagcaa taattgaatg tttcatgatg
      601 cattccttaa taagtttatt ttgttttgtt aagagtctgt atctcttgga aacaaatttc
      661 tcacatcaaa aaggttgccg caatgtaaac atattgtttt gttgatttaa aacaaattaa
      721 ctaatgcaat ataaatacaa caaataattc g
//
LOCUS       DAZFAB010000368          750 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZFAB010000368 DAZFAB010000000
VERSION     DAZFAB010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.373"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            68..750
                     /locus_tag="ACHJWA_005188"
                     /pseudo
     CDS             68..750
                     /locus_tag="ACHJWA_005188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551287.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
BASE COUNT          207 a          145 c          190 g          208 t
ORIGIN      
        1 tgcttttttt tggataacgg cacttattga tatattcatg aagattataa tcacaaggga
       61 atacataatg aaaagaaaag ttctggcaat gctggtcccg gcgttattag ttgctggcgc
      121 agcaaatgcg gctgaaattt ataataaaga tggcaataaa ctggatttgt acggaaaagt
      181 agcgggcctg cactacttct ctgatgatgc tagcagcgat ggcgacatgt catatgcccg
      241 tatcggtttc aaaggtgaaa ctcagatcgc tgaccaattc actggttatg gtcagtggga
      301 atttaacatt ggcgcaaacg gtcctgaaag cgacaagggt aataccgcaa cgcgtctggc
      361 atttgcaggt ttcggctttg gtcagaatgg tactttcgac tatggtcgta actacggtgt
      421 cgtatatgac gtagaagcat ggaccgatat gctgccagaa tttggtggag atacctatgc
      481 tggcgctgac aacttcatga acggtcgtgc taacagcgta gcaacctatc gtaacaatgg
      541 tttctttggt caagttgatg gtctgaactt tgcactccag tatcagggta acaacgagaa
      601 aagcggatta tttgatcaag aaggttcagg taacggtaat ggacgtaaac ttgctaaaga
      661 gaacggcgac ggttcagtat gtccacttcc tatgactttg actttggttt aagtctgggc
      721 gctgcttact ctaactctga ccgtaccgac
//
LOCUS       DAZFAB010000369          733 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZFAB010000369 DAZFAB010000000
VERSION     DAZFAB010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.374"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>733
                     /locus_tag="ACHJWA_005189"
     CDS             <1..>733
                     /locus_tag="ACHJWA_005189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO4311577.1"
                     /translation="QLLAASRGEFVAGISDPNDPKWVKGIETRDSVNQNQQETEQNDQ
                     KAEQNSPNTQQNEPETKQPEPVAQQEPEKVCTACGQTGGGNCPDCGAVMGDATYQETF
                     DEEYQVEVQEDDPEEMEGAEHPHKENTDGNQHHDSDNETGETADHSIKVNGHQEITST
                     SRTCDHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTIDLETAGGVIDRDT
                     IKWWLKQSREAQSAIMTDEIPLDDAL"
BASE COUNT          240 a          172 c          193 g          128 t
ORIGIN      
        1 atcagttgct cgccgcatca cgtggtgaat ttgttgcagg gattagcgac ccgaatgatc
       61 cgaaatgggt gaaggggatt gaaacccgcg attctgtgaa ccagaaccag caagaaacgg
      121 aacagaacga ccagaaagcg gaacaaaaca gcccaaatac gcaacaaaac gagccagaaa
      181 cgaaacaacc tgagccagta gcgcaacagg aaccggaaaa agtctgcacc gcctgcggtc
      241 agaccggcgg cggcaactgc cctgattgtg gcgcggtgat gggcgacgca acataccagg
      301 aaacattcga tgaagagtat caggttgaag ttcaggaaga tgatccggag gaaatggaag
      361 gcgctgaaca tccacacaag gagaacactg acggcaatca gcatcacgat agcgataatg
      421 aaactggcga gacggcagat cactcaatta aggtgaacgg tcatcaagaa atcacatcca
      481 ccagcaggac gtgtgaccat ctaatgatcg accttgaaac catgggaaaa aatcctgatg
      541 ccccgatcat ctcaataggt gcaatatttt tcgatccgca aaccggagat atgggaccgg
      601 aatttagtaa gactatcgat ctggaaactg ctggcggggt cattgatcgg gacaccatta
      661 aatggtggct taagcaatca cgcgaagcgc aatctgccat tatgaccgat gaaatcccgt
      721 tagatgatgc act
//
LOCUS       DAZFAB010000370          731 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZFAB010000370 DAZFAB010000000
VERSION     DAZFAB010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.375"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>731
                     /locus_tag="ACHJWA_005190"
     CDS             <1..>731
                     /locus_tag="ACHJWA_005190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4765 family protein"
                     /protein_id="HFO4311578.1"
                     /translation="PRNARISKELLLLAVDRQIEWAKKHFITKDVLENVVSKCDLSSI
                     FNVNKVLQNTILEFVHEINNISSARWMSKSEKNNKQKEAIEKFKKEVSHMNGGQQFIW
                     GFDKVIQEGLSGLIELSIDINDSTNHRDKSSLSPDGRAVLHFLGTIWNMAMGAVPGYN
                     ALSGVSSILHSAIVKESSNICDYIQGAVRIGMDFVPGTRSDLHSRSLQIKYEALKHIE
                     KNINDNIIYHPSNNANFYSVIESID"
BASE COUNT          247 a           92 c          151 g          241 t
ORIGIN      
        1 aacctaggaa tgccagaata tctaaagaat tgcttttatt agctgttgat agacagattg
       61 agtgggcgaa aaaacatttt ataacaaaag atgtattgga aaatgttgtg tcaaaatgtg
      121 atttatcatc tatctttaat gttaataaag tgcttcagaa tactattctt gagtttgtcc
      181 atgaaattaa taatatatca tctgctcgct ggatgtcaaa atcagaaaag aataataaac
      241 aaaaagaggc aatagaaaag ttcaaaaaag aagtatccca tatgaatggc gggcagcagt
      301 ttatttgggg gtttgataag gttattcaag aaggcttaag tggattgatt gagttaagta
      361 tcgatattaa tgatagtaca aatcatcgtg ataagtcttc tctttctcct gatgggagag
      421 ctgtgttaca ttttttaggt acaatttgga atatggcgat gggagctgta cctggttata
      481 atgcattgtc tggtgtaagt agcattttac atagtgctat agtaaaagaa tctagcaata
      541 tctgtgatta tattcagggg gctgtacgta ttggaatgga ctttgttcca ggcactcgct
      601 ctgacttaca tagccgttcg ctgcagataa aatatgaggc tttgaagcat atagaaaaaa
      661 acattaatga taatattatt tatcatccga gtaataatgc taatttctat tctgtaattg
      721 agtcaattga t
//
LOCUS       DAZFAB010000371          731 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZFAB010000371 DAZFAB010000000
VERSION     DAZFAB010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.376"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..615
                     /locus_tag="ACHJWA_005191"
     CDS             <1..615
                     /locus_tag="ACHJWA_005191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFO4311579.1"
                     /translation="GQLEAWQENAMFDTGRYRRKKIRYRDEVTGKMITRDNPPIPGKQ
                     SLAKVTSIPLVSPVEFSTSSWRRAVLSLEEHHKAWLLWCYSGSICWEYQIAITQWAWN
                     EFNTQSGTRKIAGKTQERLKKLIWLAAQAVKAELFGGEGYEYKELALLVGVTTKNWSK
                     TFTRHWVAMKHIFHRLDSEALLFVMRTRSKQKAAFSKQSVAKVD"
BASE COUNT          220 a          136 c          195 g          180 t
ORIGIN      
        1 ggtcagcttg aagcctggca ggagaatgcc atgttcgata cagggcgtta caggcgaaaa
       61 aaaatccggt accgcgatga agtgactgga aaaatgataa cgcgggataa tccaccaatc
      121 ccgggaaagc aatcgctggc gaaggtgacg tcaattcctc tggtcagtcc ggttgagttt
      181 tcgacatcat cgtggcggcg ggctgttctg tctcttgaag aacatcataa agcctggttg
      241 ttgtggtgtt acagcgggag tatttgttgg gaatatcaga tcgcgataac acagtgggcg
      301 tggaatgaat ttaatactca atccggtacc agaaaaattg cagggaaaac gcaggaacgc
      361 ctgaaaaaat taatctggct ggcggcgcag gcagtaaaag cagaactttt tggtggggaa
      421 ggttatgaat acaaggagct ggcattactg gtgggagtga caactaaaaa ctggtccaaa
      481 acatttactc gtcactgggt tgcaatgaaa cacatttttc accgactgga tagtgaggct
      541 ttattgtttg taatgagaac gcgttcaaaa caaaaggcgg cattttcaaa gcaaagtgtt
      601 gcaaaagtag attgaaaggc atatatttca tgcaaatctg atattttgcc gattttgtac
      661 gtgatggcaa aagcaaacaa aacccgccca caagcgggtt tttttgtgcc acttatctcg
      721 gatagacatg g
//
LOCUS       DAZFAB010000372          722 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZFAB010000372 DAZFAB010000000
VERSION     DAZFAB010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.377"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..97
                     /locus_tag="ACHJWA_005192"
     CDS             <1..97
                     /locus_tag="ACHJWA_005192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311580.1"
                     /translation="IVFKHYPLMKVISAQQAISALKYMVDGEVYF"
     gene            504..602
                     /locus_tag="ACHJWA_005193"
     CDS             504..602
                     /locus_tag="ACHJWA_005193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004141699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311581.1"
                     /translation="MRPQSELLTLSQMQKCTCDVLHPAFDLCGGEA"
     gene            599..>722
                     /locus_tag="ACHJWA_005194"
     CDS             599..>722
                     /locus_tag="ACHJWA_005194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311582.1"
                     /translation="MNLPQDGIKLHRGNFTAVGQQIQTYLEDGKCFRMVLKPWRE"
BASE COUNT          213 a          158 c          149 g          202 t
ORIGIN      
        1 cattgttttt aaacactatc ctttaatgaa agtaatatcc gcccagcagg cgataagtgc
       61 tctcaaatat atggttgatg gtgaggttta tttttgagta ataatcactt tttcaaccgt
      121 gctatggtat gaaagtaaag taactactat gatattaact atcgagcaag aatatcccca
      181 ggcactcaac agggcgcagg tcaacgtgaa tcggtcatga cacgacaccg attttgcgga
      241 atcaatgttg cttatgaata caacatcccg cttagtacat gttcaacaca tcagcaaatt
      301 ttgaactggg tgtggcactt aactgaaaaa acatggatga cacaaaatgt tactcgtcgc
      361 ttcattgaag tagcttgtgg atatcacaag ctagactatc gccaatgata ttcaattttt
      421 gattttttag tatcaacctt ccataataat gtcaccggag cttgaacaac tccggtgact
      481 tctgcgcatt taaggggact taaatgcgac cacaatctga actccttacc ttgtcacaga
      541 tgcagaaatg cacctgcgat gtcttgcatc cagcgtttga tctctgcgga ggtgaagcgt
      601 gaacctccca caagatggca tcaaattaca tcgcggtaac ttcaccgctg tcggtcagca
      661 gatccagact tatctggaag acggcaaatg ctttcgcatg gtgcttaaac cgtggcgcga
      721 ga
//
LOCUS       DAZFAB010000373          715 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZFAB010000373 DAZFAB010000000
VERSION     DAZFAB010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.378"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..275
                     /locus_tag="ACHJWA_005195"
     CDS             <1..275
                     /locus_tag="ACHJWA_005195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO4311583.1"
                     /translation="SDHGESLGENGLYLHGTPYNVAPAEQTHVPLITWMSPGFVSSKK
                     IDLNCLSEHALNRTVSHDNIFSSLLGLWNVNTSVYNKEDDIISSCR"
     gene            complement(368..>715)
                     /locus_tag="ACHJWA_005196"
     CDS             complement(368..>715)
                     /locus_tag="ACHJWA_005196"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFO4311584.1"
                     /translation="TTGVTRIIKAAGYSWKGLCAAWRYEAAFRQECILVLSALIISFW
                     LDVDSVTRVLLIGSAMMVIIVEIINSAIEAVVDRIGSEHHELSGRAKDMGSAAVLITI
                     LVAIIIWGVLLLS"
BASE COUNT          231 a          143 c          127 g          214 t
ORIGIN      
        1 tctctgacca tggagaatct ctgggagaaa atggattata cttacatgga acaccatata
       61 atgttgctcc agctgagcaa acgcatgtgc ctcttattac ctggatgtct ccggggttcg
      121 tttcctctaa aaaaattgat ttgaattgtt tgtcagaaca tgctctaaac agaacagtaa
      181 gtcatgataa tatattttca tcgttactgg ggttatggaa tgtaaatact tcagtttata
      241 ataaagaaga tgatattata tcctcctgca gatagatact gacatttttc tgaggataag
      301 aggtgaatac ctcttatcct taagtaaata ttaaccaggt aactgtgaat aatttagatg
      361 ttgggcgtta tgaaagtaat aaaactcccc atataatgat agcaactaaa atcgtaatca
      421 acactgcggc tgaacccata tcttttgctc tgcctgataa ttcgtgatgt tctgaaccaa
      481 ttctgtcaac aactgcttca attgcactgt taattatttc aacaataatt accatcattg
      541 ccgaaccaat gagtaatact cgagtaacag agtcaacatc cagccaaaag gatattatca
      601 gtgctgacag aaccagaatg cactcctgac gaaaggctgc ttcatagcgc catgccgcac
      661 ataagccttt ccatgaataa cccgctgctt taataattct ggtaacacca gtggt
//
LOCUS       DAZFAB010000374          714 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZFAB010000374 DAZFAB010000000
VERSION     DAZFAB010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.379"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..>714)
                     /locus_tag="ACHJWA_005197"
     CDS             complement(<1..>714)
                     /locus_tag="ACHJWA_005197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001310363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III effector protein"
                     /protein_id="HFO4311585.1"
                     /translation="LSVYLKNKIPFENMIIDLSALKKDVLVSLKQCCFKKMTFTGNIS
                     YENLNGPVFENCFFEECNFESVSLVGFDKVTCESYDVLCNVKPNNKIPIYGMFKGCFL
                     YQCEMKNFKIETSKIYSINQDPQRGDKKVGAYLFMQSFVYASNLQDGVCKEASVIASS
                     LLSCNIAKLNGVGMDFIETSFYGKTRGGFRDSNNFYDCDFRYVNMTCKRDGIREDLRD
                     YDPRKYFGESENKNKLNLD"
BASE COUNT          233 a          139 c           84 g          258 t
ORIGIN      
        1 tatccagatt aagtttattt ttattttcac tttctccgaa atattttctt ggatcataat
       61 ctcgtaaatc ttcacgaatt ccatctcgtt tgcatgtcat attaacataa cgaaaatcac
      121 aatcataaaa attattacta tctctaaaac caccgcgagt ttttccataa aacgaagttt
      181 ctatgaaatc cattccaaca ccattaagct ttgctatatt gcaagataat aaactgcttg
      241 caataacact tgcttcttta cacactccat cttgaagatt acttgcatat acaaatgact
      301 gcataaacag atatgcaccg actttcttat cccctctttg gggatcttga tttatagagt
      361 atatttttga tgtctctatc ttgaaatttt tcatttcgca ttgatacaaa aaacaccctt
      421 taaacatacc gtaaatcggt attttattat ttggttttac gttacaaaga acgtcatagc
      481 tttcacaggt cactttatca aagccaacaa gggacacact ctcgaaatta cattcctcaa
      541 aaaaacaatt ttcaaatact gggccattaa gattttcgta agatatatta cctgtgaatg
      601 tcatcttttt aaagcaacac tgttttagcg ataccagaac atcttttttc agtgcagata
      661 aatcgattat catattttca aaggggattt tattttttaa atatacgctt agtt
//
LOCUS       DAZFAB010000375          713 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZFAB010000375 DAZFAB010000000
VERSION     DAZFAB010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.380"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..>713)
                     /gene="espX2"
                     /locus_tag="ACHJWA_005198"
     CDS             complement(<1..>713)
                     /gene="espX2"
                     /locus_tag="ACHJWA_005198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspX2"
                     /protein_id="HFO4311586.1"
                     /translation="FIPYVNTFPDLKNKILRMKIELTEGSGFSKSLSDSQIEMHILRT
                     VNSLNVGEKLNDDNLADHSIFTNEFSVIIPPSYYDATSAVNANNIVREKLFESDSKVK
                     DIVDDMSNHDVESEKDIFVIGGMIEKLNSLADESFNDSTDNIQTVKDLLTQLTDGMEL
                     FALRDIVAFPSTIIAKLIKSPLNSDHELVMRALDTYLCYFRNKNLNNNAEIINFFHAL
                     FLKRPELMVAENYRFIQFI"
BASE COUNT          221 a          131 c          115 g          246 t
ORIGIN      
        1 tcaataaatt gaataaatct ataattttca gcaaccatca actctggtct ctttaaaaag
       61 agggcatgga aaaagttaat tatttccgca ttgttattca gatttttatt tctgaagtag
      121 cacagatacg tatccagtgc gcgcataaca agttcatgat cgctattaag tggggatttt
      181 attaattttg ctattatggt tgatgggaat gccactatgt cccgcagcgc gaagagctcc
      241 atgccatcag ttaattgcgt taacagatct tttactgttt gtatgttatc agtactatca
      301 ttgaaagatt cgtctgccag agaattcagt ttttctatca tccccccgat tacaaatata
      361 tctttttcgc tctccacgtc atgattagac atatcatcga cgatatcttt aactttacta
      421 tcactttcaa atagtttttc tctcacaata ttattagcgt ttactgcaga agttgcatca
      481 taataggatg gcggaataat aacagagaat tcgttagtga atatagaatg atctgcaaga
      541 ttatcatcat tcaatttctc gccaacattt agcgaattaa cagtcctcag aatatgcatc
      601 tcaatctgac tgtcagaaag acttttagaa aaaccactac cttcagtcag ttcaattttc
      661 atcctgagta ttttattttt gaggtcggga aatgtattta cataaggaat aaa
//
LOCUS       DAZFAB010000376          712 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZFAB010000376 DAZFAB010000000
VERSION     DAZFAB010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.381"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..266)
                     /locus_tag="ACHJWA_005199"
     CDS             complement(<1..266)
                     /locus_tag="ACHJWA_005199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO4311587.1"
                     /translation="MATTNAFCLASPPLARICLHGDLQRFGRRLSLYVNTAAEAIRAL
                     SLQMPRFRRQMNEGWYQIRIRGEDTAPEAVYARLHEQLGEGTVI"
     gene            complement(164..>712)
                     /locus_tag="ACHJWA_005200"
     CDS             complement(164..>712)
                     /locus_tag="ACHJWA_005200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFO4311588.1"
                     /translation="LVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGEIHKFRCVPHL
                     TGRRFEHGVTDCYTLFRDAYHLAGITLPDFVREDDWWRNGQNLYLDNMAATGFYRVPL
                     SSAQAGDILLCCFGASVANHAAIYCGNGELLHHLPEQLSKRERYSEKWQRRTHSVWRH
                     RHWHASAFTGICNDLAAASACT"
BASE COUNT          148 a          224 c          203 g          137 t
ORIGIN      
        1 tggatgaccg ttccctcacc cagctgttcg tgaagacggg cgtacaccgc ctccggtgcc
       61 gtgtcctcac cgcgaatacg tatctggtac cagccttcgt tcatctgacg gcggaatctc
      121 ggcatctgca gcgacagggc acggatggct tccgctgccg tgttcacgta caggctgagg
      181 cggcggccaa atcgttgcaa atccccgtga aggcagatgc gtgccagtgg cggtgacgcc
      241 agacagaatg cgttcgtcgt tgccattttt cagaatacct ctcccgttta ctcagttgtt
      301 caggcagatg gtgaagcagt tcaccgttgc cgcagtatat ggcggcatga ttggccaccg
      361 atgcgccaaa gcagcacagc aggatatcgc ccgcctgtgc agaggacagg ggcacccggt
      421 aaaaaccagt cgccgccata ttgtccaggt aaaggttctg accgttgcgc caccagtcat
      481 cctcacgcac aaaatccggc agcgttatcc ccgccagatg gtatgcatcc cggaacaggg
      541 tgtaacagtc cgtcaccccg tgctcaaaac gacgcccggt caggtgcggc acacagcgga
      601 atttgtgaat ttccccccgg cagaccagcc accagggcag ggcactcttt atctgcagcc
      661 gccggtcggc ctcgctcagc cagggcagac caccagggtg gctgtgaacc ag
//
LOCUS       DAZFAB010000377          696 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZFAB010000377 DAZFAB010000000
VERSION     DAZFAB010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.382"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>696
                     /locus_tag="ACHJWA_005201"
     CDS             <1..>696
                     /locus_tag="ACHJWA_005201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO4311589.1"
                     /translation="DWLKKEIQRVYDENHQVYGVRKVWRQLLREGIRVARCTVARLMA
                     VMGLAGVLRGKKVRTTVSRKAVAACDRVNRQFVAERPDQLWVADFTWVSTWQGFVYVA
                     FIIDVFAGYIVGWQVSSSMETTFVLDALEQALWARRPSGTIHHSDKGSQYVSLAYTQR
                     LKEAGLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWKNRTEVELATLTWVDWYNN
                     RRLLERLGHIPPAE"
BASE COUNT          173 a          159 c          213 g          151 t
ORIGIN      
        1 gactggctga agaaagagat acagcgcgta tacgatgaaa atcaccaggt atacggtgtg
       61 cgtaaagtct ggcgtcagtt gttacgggaa ggtatcagag tggccagatg cactgtggca
      121 cgtctcatgg cagttatggg acttgccggt gttctccggg gtaaaaaggt ccgcactacc
      181 gtcagccgga aagccgttgc cgcatgcgac cgcgtaaacc gtcagttcgt ggcagaacgt
      241 ccagaccagt tgtgggtggc tgattttacc tgggtaagca catggcaggg cttcgtttat
      301 gtggcgttca tcattgatgt gttcgccgga tacatcgtgg gatggcaggt ctcatcatcc
      361 atggaaacaa cattcgtgct ggatgcactg gagcaggcgt tgtgggcccg tcggccgtcc
      421 ggcacaatcc atcacagtga taaaggttct cagtatgtat cgctggccta cacgcagcgg
      481 cttaaggaag ccggattact ggcatcaaca gggagtactg gcgactcgta tgacaacgcg
      541 atggctgaga gcatcaatgg tctttacaaa gcggaggtaa tacaccgtaa gagctggaaa
      601 aaccggacag aagtggaact ggccacacta acgtgggtgg actggtataa caatcgacga
      661 ttgctggaaa ggctgggcca tattcctccg gcagaa
//
LOCUS       DAZFAB010000378          688 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZFAB010000378 DAZFAB010000000
VERSION     DAZFAB010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..688
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.383"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..>688)
                     /gene="espL2"
                     /locus_tag="ACHJWA_005202"
     CDS             complement(<1..>688)
                     /gene="espL2"
                     /locus_tag="ACHJWA_005202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspL2"
                     /protein_id="HFO4311590.1"
                     /translation="DGTIPGLYQALQNGHAQAIKSYGNLVLDTINKNIDLEYLLSAFK
                     YEAHSSNKYTPGLFSAFQNGHADAIKAYCGVLGNSNLKRGEIIRMLEARNYDGAPGLL
                     LAYQNGDINTIQSFFDSLIMLDISKDFIEELLTAKHYDFTGLSLAISHRHDHVVKLYG
                     KLFKKLDTSPYKMSIILALAIDCERNNANIIIDSEYKSNKAVKEYVEILKEFNICPEK
                     VAEYLSEFSGK"
BASE COUNT          211 a          134 c           93 g          250 t
ORIGIN      
        1 ttttccgctg aactcgctta ggtattctgc taccttttct gggcatatat tgaattcttt
       61 taaaatctct acatattcct ttactgcctt atttgacttg tactcactat caataattat
      121 gtttgcatta tttctctcgc aatcaattgc taatgctagg ataattgaca ttttatatgg
      181 actggtatca agttttttaa ataactttcc gtataatttg acaacatgat catgtctatg
      241 actgatcgct aatgataacc ccgtaaaatc ataatgtttt gcagtcaata actcttcgat
      301 aaaatccttt gatatatcta acattattag tgagtcaaaa aaagactgaa tcgtattaat
      361 atctccattt tgatacgcaa gaagtaatcc tggtgcacca tcataatttc tcgcctcaag
      421 catcctgatt atctcacctc ttttcaaatt actattgcca agtacgccac aatatgcctt
      481 tattgcgtct gcatgaccat tttgaaatgc agaaaataat ccaggagtat atttattaga
      541 gctatgtgcc tcatatttaa atgctgaaag caaatactct aaatctatat ttttattaat
      601 agtatccaga actaagttac cataactctt aatagcttga gcatgtccat tttgaagggc
      661 ctgatataaa ccaggtatgg ttccatcc
//
LOCUS       DAZFAB010000379          682 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZFAB010000379 DAZFAB010000000
VERSION     DAZFAB010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.384"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>682
                     /locus_tag="ACHJWA_005203"
     CDS             <1..>682
                     /locus_tag="ACHJWA_005203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4311591.1"
                     /translation="VVKGVSFMLRLTVAADDGSERLVSTARTTETTYRFTQLAPGNYR
                     LTVRAVNAWGQQGDPASVSFRIAAPAAPSQIELTPGYFQITAVPKLAVYDPTVQFEFW
                     FSEAKIADTSQVETSARYLGTGSQWSVSGPHIKPGKDFWFYVRSVNLVGKSAFVEVSG
                     QPSNDGEGYLEFFREKIGKLHLAQGLWELIDNSQLADEMAEMKTSITETRNEITQTVS
                     KTLEDQSAT"
BASE COUNT          163 a          167 c          223 g          129 t
ORIGIN      
        1 aggtggtgaa gggcgtgagc tttatgcttc gcctgaccgt ggccgcggat gacggcagtg
       61 agcggctggt cagcacggcc cggacgacgg aaaccacata ccgcttcacg caactggcgc
      121 cggggaacta caggctgaca gtccgggcgg taaatgcgtg ggggcagcag ggcgatccgg
      181 cgtcggtatc gttccggatt gccgcaccgg cagcgccgtc acagattgag ctgacaccgg
      241 gctattttca gataacggcg gtcccgaaac tggctgtata tgacccgacg gtacagtttg
      301 aattctggtt ttcggaggca aaaatcgcag acacatctca ggtggaaacc tctgcccgtt
      361 atctggggac cggcagtcag tggagtgtat ccggcccgca cattaagccc gggaaggatt
      421 tctggtttta cgtgcgcagc gtcaacctgg tggggaaatc tgcgtttgtg gaagtcagcg
      481 ggcagcccag caatgatggt gaagggtatc tggaattttt ccgggaaaaa ataggaaaac
      541 tgcatctggc tcaggggttg tgggaactga tagataacag ccagcttgcg gatgagatgg
      601 cggagatgaa gaccagcatc acggaaaccc gcaatgaaat cacacagacg gtcagtaaaa
      661 cgctggagga ccagagcgcc ac
//
LOCUS       DAZFAB010000380          671 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZFAB010000380 DAZFAB010000000
VERSION     DAZFAB010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.385"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..505)
                     /locus_tag="ACHJWA_005204"
     CDS             complement(<1..505)
                     /locus_tag="ACHJWA_005204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO4311592.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAEYLHARTSAPG
                     SDNLNGINVKYRYEFTDTLGMVTSFSYAGDRNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNP"
     gene            complement(573..>671)
                     /locus_tag="ACHJWA_005205"
     CDS             complement(573..>671)
                     /locus_tag="ACHJWA_005205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J protein"
                     /protein_id="HFO4311593.1"
                     /translation="SAWLVNDWYPTASISDLLVVVMKKATAGISIS"
BASE COUNT          155 a          198 c          165 g          153 t
ORIGIN      
        1 tcgggttaaa ctgcacgcca gctccccacg ccagagacgt gttgctgtgg cgaccgtcat
       61 cacttccggt cagcacatca tgcgttttcc ccttgttgtc agttacgcgg agataatccc
      121 cggagaaagt cgacacacgg ctgtaagcca cacccgccat cgcatacgcg ctgaaccatt
      181 cattcacgcg cacagagggc cccgccatca cgctgaacca gcggttacgc acggaatctt
      241 catgccagcg ggtatcgctg taacgggtaa gctggcgatt cctgtctcct gcatagctga
      301 atgacgtcac catccccagt gtgtccgtaa actcataacg gtatttcacg ttaatcccgt
      361 taagattatc gctaccggga gcgctcgtcc gggcatgaag gtactccgcg ctcagcgtgg
      421 actgatgttc agacgcccat gcaggcgcgc cggatacggc cagacagatg gctgcggaca
      481 aaatggcggc ataaagttta cgcataatta cctctcgctt ttctgcaata aaaaaggcgc
      541 catttctggc gcccgtatat gggttataaa attcagctga tactgatgcc tgcggtggct
      601 ttcttcatca ccacaaccag caaatcgctg atacttgctg tgggatacca gtcatttacc
      661 agccatgctg a
//
LOCUS       DAZFAB010000381          667 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZFAB010000381 DAZFAB010000000
VERSION     DAZFAB010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..667
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.386"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>667
                     /gene="escU"
                     /locus_tag="ACHJWA_005206"
     CDS             <1..>667
                     /gene="escU"
                     /locus_tag="ACHJWA_005206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     switch protein EscU"
                     /protein_id="HFO4311594.1"
                     /translation="FVGTVTTGVSQIGFIFAVEKIKPSAQKISVKNNLKNIFSVKSIF
                     ELLKSVFKLVIIVLIFYFMGHSYANEFANFTGLNAYQALVVVAFFVFLLWKGVLFGYL
                     LFSVFDFWFQKHEGLKKMKMSKDEVKREAKDTDGNPEIKGERRRLHSEIQSGSLANNI
                     KKSTVIVKNPTHIAICLYYKLGETPLPLVIETGKDAKALQIIKLAELYDIPVIEDIPL
                     ARTL"
BASE COUNT          213 a          108 c          131 g          215 t
ORIGIN      
        1 tttgttggaa ctgttacgac tggtgtatca caaataggat tcatctttgc ggttgaaaaa
       61 ataaaaccat cggctcagaa gattagtgta aaaaataacc tgaaaaatat tttttctgta
      121 aagagcattt ttgagctact taaatcagta tttaagttag tgataattgt tctcattttt
      181 tattttatgg ggcattcata tgcaaatgag tttgctaatt tcacaggact gaacgcatat
      241 caagctcttg tcgttgttgc cttttttgtt tttcttttat ggaaaggcgt gctattcgga
      301 tatctactct tttcagtatt tgatttctgg ttccagaagc atgagggact gaagaaaatg
      361 aaaatgagta aagatgaggt gaaacgagaa gccaaggata ctgatggtaa ccctgaaatt
      421 aaaggggagc gccgtcgcct tcattccgag atacaaagtg gaagtttggc taataacatc
      481 aaaaaatcaa ccgttattgt taaaaacccg actcacattg cgatttgcct atactataaa
      541 cttggggaga ctccattacc tttagttatt gaaacaggaa aagatgccaa agctctacag
      601 atcattaaac tggctgaact ctatgatatt ccagtgattg aagatattcc tttagcaaga
      661 actctct
//
LOCUS       DAZFAB010000382          666 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZFAB010000382 DAZFAB010000000
VERSION     DAZFAB010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.387"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>666
                     /gene="yjgL"
                     /locus_tag="ACHJWA_005207"
     CDS             <1..>666
                     /gene="yjgL"
                     /locus_tag="ACHJWA_005207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YjgL"
                     /protein_id="HFO4311595.1"
                     /translation="ELNNSQELNNSQDLKNSQVSCKGSVDFTITDLLEKSLNNALLAI
                     RNEHLLLMPHVCSESISYLLGENGILEEIDKLYELNDHGIDNDKEGNNEINDIMINLS
                     HILIESLDDAKVNLTPVIHSMLMTFLELPYNNDVKILEWCFNKSMQYFDDSAKIEHAC
                     SVINHINFRRDQSKVAETLFFNLDKEPYKNSPELQELIWKKLVVYVNDFNLSNREKTY
                     LIQ"
BASE COUNT          246 a           89 c          120 g          211 t
ORIGIN      
        1 aggagttaaa taattcgcag gagttaaata actcgcagga cttaaaaaat tcgcaggtga
       61 gttgtaaagg ttcagttgat tttacgatta cggatttatt agaaaaatca ttgaataatg
      121 cattattagc aataaggaac gaacatctgc tattaatgcc tcatgtatgt agtgaatcga
      181 tttcatactt actgggcgaa aatggtatac ttgaagaaat agataagctc tacgaattaa
      241 atgatcacgg aattgataat gacaaagaag gtaacaatga aattaatgac atcatgatta
      301 acctgtctca tattcttatt gaatccttag atgatgcaaa ggttaatctt acaccggtca
      361 tccattcgat gttgatgact tttttagaat tgccatataa taatgatgta aaaatactgg
      421 agtggtgttt taataaaagc atgcaatatt ttgatgattc tgcaaagata gagcatgcat
      481 gctccgtaat aaatcatatt aattttcgtc gcgatcagtc taaagtagct gagacattat
      541 ttttcaatct cgataaagaa ccctataaaa atagccctga attacaggag ttgatttgga
      601 aaaagttggt tgtatatgtc aatgatttta acttaagcaa tcgagaaaaa acatatttaa
      661 tacaaa
//
LOCUS       DAZFAB010000383          661 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZFAB010000383 DAZFAB010000000
VERSION     DAZFAB010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.388"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
BASE COUNT          219 a          142 c          132 g          168 t
ORIGIN      
        1 caaataaaac agaataaaaa ccaagctgaa ccgtttcact atactctact taattcgagt
       61 tacaggaagg cagcaagcga gtgaatcccc aggagcttac ataagtaagt gattggggta
      121 agcgaacgca gtcgcagcac atgcaacttg aagtatgacg agtatatata tctattagct
      181 gtttcccgga cataagctac agccagcact aacctggaac gggtgcatta taaaaaggaa
      241 caagggacaa atagctgaac agtaaccgcg ttagtctctt cccgcataac cgtaccattc
      301 gtattaagaa gtattgccca tatccattcg gcgaaagagg atctgccata catcaagtcc
      361 gttttacttg gcatcagatc atcgccgtca ttaactctgt taaagtcgga caaacgatta
      421 gcaagatctg tgattcaaaa gtgcttagcg ctaaggtggg actggttaca gattcattaa
      481 ccctgttctt tgtcgcacct atcgatcggt gtaatgccac aacaggttta tgttaatcag
      541 aacaatgcgc ccctctataa agagaattct ttgataaggc aacttgaaaa gggaaagcta
      601 actgatatca atgtaccact ttagtaatca tcacattata tacaggccaa aataagtttc
      661 c
//
LOCUS       DAZFAB010000384          656 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZFAB010000384 DAZFAB010000000
VERSION     DAZFAB010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.389"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..93
                     /locus_tag="ACHJWA_005208"
     CDS             <1..93
                     /locus_tag="ACHJWA_005208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFO4311596.1"
                     /translation="TREPITASIIVKHEECIYDDTRGNFVIKGN"
     gene            166..>656
                     /locus_tag="ACHJWA_005209"
     CDS             166..>656
                     /locus_tag="ACHJWA_005209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFO4311597.1"
                     /translation="MPVTTLSIPSISQLSPAGVQSLQDAARLESGIRISIGSGQYSVH
                     YVQLLDGFSVEPVRGGLLDRLLGREHRMDRRAVALERQLNGGVDFLSSVNNYFQSVMA
                     EHRENKTGNKILMEKINSCVFGTDSNHFSCPESFLTCPITLDKPENGVFMRNSQGAEI
                     CSL"
BASE COUNT          213 a          106 c          145 g          192 t
ORIGIN      
        1 acccgggaac caataacggc atcaataatt gtaaaacatg aagaatgcat ttatgacgat
       61 accagaggaa acttcgttat aaagggtaat tgaaatgaac attacccttt attttattta
      121 atgaaacatc ctgcaaactg atatgaatta ctgaatgagg tttttatgcc tgttaccacc
      181 ttaagtatcc caagtatatc tcaattatct cctgcaggag tacagtcttt gcaggatgct
      241 gccagacttg aaagtggaat aagaatatcc attggtagtg gccaatattc tgttcactat
      301 gtccaactac tggatggatt ttcagttgaa ccggtgagag gaggcttact ggataggcta
      361 ttggggcgtg agcatcgaat ggatagaagg gctgtggctc tggaaaggca attaaatgga
      421 ggtgtcgatt ttttaagtag tgttaataac tattttcaga gtgtcatggc agaacacaga
      481 gaaaataaaa caggtaataa aatattaatg gaaaaaataa attcttgtgt atttggaacg
      541 gattctaatc acttttcttg cccggagtca tttttgacat gcccgataac gctggacaaa
      601 cctgagaatg gagtgttcat gagaaactca caaggtgctg agatatgctc tctata
//
LOCUS       DAZFAB010000385          653 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZFAB010000385 DAZFAB010000000
VERSION     DAZFAB010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.390"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..327
                     /locus_tag="ACHJWA_005210"
     CDS             <1..327
                     /locus_tag="ACHJWA_005210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO4311598.1"
                     /translation="AGASMALWGSALAAGGFSATTMLFSLGASMILGGVAQMLAPKAK
                     VPEYKSTDNGRQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRDEGGDGKV
                     VVIGRG"
BASE COUNT          172 a          137 c          206 g          138 t
ORIGIN      
        1 gccggggcat caatggcgtt atggggttca gccctggcag ccggtggttt ttctgccacc
       61 acgatgctgt tttcactggg ggccagcatg atactgggtg gtgtggctca gatgctggcc
      121 ccgaaggcta aagtaccgga gtacaaaagc acggataacg gcagacagaa cacgtacttt
      181 tcctcgctgg ataacatgat tgcacagggg aacccgatgc cggtgcctta cggtgaaatg
      241 ctggttggct cacggcgaat ctcccaggac atcagtaccc gtgatgaagg cggagacggg
      301 aaagtggtgg ttatcgggcg gggatgaaaa taaaaaaatc ccgcagagtt agcggagctg
      361 cgggagagaa cgatgaagat taacgttatg gagttatttt tcaggcatca aaaaagtaat
      421 gcagcgtcat tattgcggct acaggcaatt gccggaaatg tgaagagttt cagaaatttt
      481 attccgtcat gacacaggca ccctccgggg tgcctgttgt tttctggcat aaacagattc
      541 agacatcaga caggagaggg ggacagagtg ggtaaagggg gcggcaaggc gcacacgccg
      601 gttgaggcaa aggacaatct taagtccacg cagatgatga gcgtgattga tgc
//
LOCUS       DAZFAB010000386          625 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZFAB010000386 DAZFAB010000000
VERSION     DAZFAB010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.391"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>625
                     /locus_tag="ACHJWA_005211"
     CDS             <1..>625
                     /locus_tag="ACHJWA_005211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFO4311599.1"
                     /translation="DALDRLVQQRGFDGRTQRYHYDLTGKLTQSEDEGLVTLWHYDES
                     DRLTHRTVNGEPAEQWQYDEHGWLTEISHLSEGHQVAVHYGYDDKGRLAGERQTVHNP
                     ETGELLWQHETEHAYNEQGLANRVTPDSLPRVEWLTYGSGYLAGMKLGGTPLVEFTRD
                     RLHRETVRSFGNNAYELTSTYTPAGHLQSQRLNSQVYDRDYDWNDNGD"
BASE COUNT          148 a          179 c          208 g           90 t
ORIGIN      
        1 gatgcgcttg accggctggt acagcagcgc ggctttgacg ggcggacgca acgttaccac
       61 tatgacctga ccggaaaact cacgcagagt gaagatgagg ggcttgtcac cctctggcac
      121 tacgacgaat cggaccgcct cactcaccgc acggtgaacg gcgaaccggc agagcagtgg
      181 cagtacgacg agcacggctg gctgacagaa atcagccacc tgagcgaagg ccatcaggtg
      241 gcggtgcatt acggttatga tgataagggc cgcctggccg gggagcgcca gacggtgcat
      301 aacccggaga cgggggaact gctgtggcag catgagacag agcacgcata caacgaacag
      361 ggtctggcaa accgcgtcac gccggacagc ctgccgcggg tggagtggct gacctacggc
      421 agcggttatc ttgcggggat gaagctgggc gggacgccgc tggtggagtt cacgcgcgac
      481 aggctgcacc gcgagacggt gcgcagcttc ggcaataacg catacgaact gaccagcaca
      541 tacactcccg caggccattt acagagccag cgcctgaaca gccaggtgta tgaccgtgac
      601 tacgactgga atgacaatgg cgacc
//
LOCUS       DAZFAB010000387          624 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZFAB010000387 DAZFAB010000000
VERSION     DAZFAB010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.392"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..589)
                     /locus_tag="ACHJWA_005212"
     CDS             complement(<1..589)
                     /locus_tag="ACHJWA_005212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HFO4311600.1"
                     /translation="MDTPDEKERITFPQVSRRSFLQATSALITLPFISSTAKAQSPDA
                     SPEVTAPVADKVVPTCSTFDCGGKCDIRAHMRDGVVTQITTLPDNELDPQMPIMRACV
                     RGRGYRKFVYHPDRLKYPMKRVGKRGEGKFERISWDEATTLIADNLKRITQQYGPASR
                     YVHVGTAVFGGTFSGDAMARRLLNLTGGYLEYYHSV"
BASE COUNT          173 a          156 c          140 g          155 t
ORIGIN      
        1 taactgagtg ataatattca agataaccac cggttaaatt taacagccga cgcgccatcg
       61 catcgccaga aaatgttcca ccaaaaacag ccgtcccgac atggacataa cgtgaagctg
      121 ggccatattg ttgcgtgatc cttttcaggt tatcggcaat taaggtcgtc gcctcatccc
      181 atgagatacg ctcaaattta ccctcgcctc gtttacctac ccgcttcatc ggatatttca
      241 aacggtctgg atgataaaca aatttacgat agccgcgacc acgaacacag gcgcgcataa
      301 tcggcatttg tggatccaat tcgttatcgg gaagcgtggt aatttgcgtc accacgccgt
      361 cgcgcatatg cgcccgaata tcgcacttac caccgcagtc aaatgtgcta caggtgggca
      421 cgactttatc agctactgga gctgtaactt ctggcgacgc atctggggat tgcgcctttg
      481 cggtagaaga aataaaaggc aaagtgataa gcgcggaagt cgcttgtaaa aaactacgtc
      541 gtgatacttg cggaaatgta atgcgttcct tttcgtctgg cgtatccatc ttgcgttccc
      601 gaaaatgaaa aaataaatat tcac
//
LOCUS       DAZFAB010000388          619 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZFAB010000388 DAZFAB010000000
VERSION     DAZFAB010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.393"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>619
                     /locus_tag="ACHJWA_005213"
     CDS             <1..>619
                     /locus_tag="ACHJWA_005213"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047234906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS30 family transposase"
                     /protein_id="HFO4311601.1"
                     /translation="GTIFTMLRDTGGIKPHERKRAVAHLTLSEREEIRAGLSAKMSIR
                     AIATALNRSPSTISREVQRNRGRRYYKAVDANNRANRMAKRPKPCLLDQNLPLRKLVL
                     EKLEMKWSPEQISGWLRRTKPRQKTLRISPETIYKTLYFRSREALHHLNIQHLRRSHS
                     LRHGRRHTRKGERGTINIVNGTPIHERSRNIDNRRSLGHWEGDLVS"
BASE COUNT          189 a          145 c          155 g          130 t
ORIGIN      
        1 ggaacgatct tcactatgtt aagggatact ggcggcataa aaccccatga gcgtaagcgg
       61 gctgtagctc acctgacact gtctgagcgc gaggagatac gagctggttt gtcagccaaa
      121 atgagcattc gtgcgatagc tactgcgctg aatcgcagtc cttcgacgat ctcacgtgaa
      181 gttcagcgta atcggggcag acgctattac aaagctgttg atgctaataa ccgagccaac
      241 agaatggcga aaaggccaaa accgtgctta ctggatcaaa atttaccatt gcgaaagctt
      301 gttctggaaa agctggagat gaaatggtct ccagagcaaa tatcaggatg gttaaggcga
      361 acaaaaccac gtcaaaaaac gctgcgaata tcacctgaga caatttataa aacgctgtac
      421 tttcgtagcc gtgaagcgct acaccacctg aatatacagc atctgcgacg gtcgcatagc
      481 cttcgccatg gcaggcgtca tacccgcaaa ggcgaaagag gtacgattaa catagtgaac
      541 ggaacaccaa ttcacgaacg ttcccgaaat atcgataaca gacgctctct agggcattgg
      601 gagggcgatt tagtctcag
//
LOCUS       DAZFAB010000389          612 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZFAB010000389 DAZFAB010000000
VERSION     DAZFAB010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.394"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..330
                     /locus_tag="ACHJWA_005214"
     CDS             <1..330
                     /locus_tag="ACHJWA_005214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO4311602.1"
                     /translation="AGASMALWGSALAAGGFSATTMLFSLGASMILGGVAQMLAPKAK
                     TPDYRATDNGRQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRDEGGDGKV
                     VVIGRQA"
BASE COUNT          160 a          141 c          191 g          120 t
ORIGIN      
        1 gccggggcat caatggcgtt atggggttca gccctggcag ccggtggttt ttctgccacc
       61 acgatgctgt tttcactggg ggccagcatg atactgggcg gtgtggccca gatgctggca
      121 ccgaaggcaa aaacaccgga ttaccgcgca acggataacg gcagacagaa cacgtacttt
      181 tcctcactgg acaacatgat tgctcagggg aacccgatgc cggtgcctta cggtgaaatg
      241 ctggttggct cacggcgaat ctcccaggac atcagtaccc gtgatgaagg cggtgacggg
      301 aaggtggtgg ttatcgggcg gcaggcataa aagcgaaaaa atcccgcagt gaccgaaggc
      361 tgcgggaaca gaaaatgaag attaaccaca gggagttttg tttttattgg cccgaaaaaa
      421 ctgtaacgcc cgggaatgat atctgccacg ggggcgtaca gaaaatgtga agaaattcag
      481 aaattttatt ccgtcatgac acaggcaccc tccggggtgc ctgtcgtttt tggggcataa
      541 acagattcag acatcagaca tcagacatca gacaggagag ggggaccgag tgggtaaagg
      601 tggcggcagg gc
//
LOCUS       DAZFAB010000390          612 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZFAB010000390 DAZFAB010000000
VERSION     DAZFAB010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.395"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            52..>612
                     /locus_tag="ACHJWA_005215"
     CDS             52..>612
                     /locus_tag="ACHJWA_005215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFO4311603.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLTGEGAARQNAINASLAQGVEASLRWLTEMTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLADQVHHKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGA"
BASE COUNT          145 a          155 c          174 g          138 t
ORIGIN      
        1 tgagctcatt actgacagta aggcgctgag ggaagagctg gagacaatgc tgtgacttca
       61 ccatttattc aacaaattgc tgataaccgg gtatgtcagg tactcacctg tcttcctgaa
      121 aaatttgtgg ttgatttcgc aaacggaatc gatgttgcac aggagcatat ccgcacggcc
      181 ggagagcgca cgttcttccg gcgtttaaaa gaggggctga ccggcgaagg cgctgcacgg
      241 cagaatgcca tcaatgcgtc gcttgcacag ggcgttgagg cgtccctgcg ctggctgacg
      301 gagatgacaa cgtcacttgc caccacaaac tacgcgatta cccgggtaaa cgacagggtc
      361 agttcactgg tcagtgatac agccaggctg gcgcattatt ccgcagatac gcgggagcag
      421 ttactcaccc tggccgatca ggttcaccat aaactgaatc atcttgaaga aaaactccac
      481 cgtgttgacc aggttcagcg ggcgcagtta catcttgagc agatattctc atggtggagc
      541 gccgggcgat atgcgtcatt ttcccctgcc ggacgttgtt atgtggcact tgaagagctt
      601 cgctggggcg cg
//
LOCUS       DAZFAB010000391          608 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZFAB010000391 DAZFAB010000000
VERSION     DAZFAB010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.396"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..71
                     /locus_tag="ACHJWA_005216"
     CDS             <1..71
                     /locus_tag="ACHJWA_005216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO4311604.1"
                     /translation="RAIKAEYQQKLKKLRNSRSEVA"
     gene            68..>608
                     /locus_tag="ACHJWA_005217"
     CDS             68..>608
                     /locus_tag="ACHJWA_005217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFO4311605.1"
                     /translation="MNIYERIDGSKYRNIWVVGDLHGCYTNLMKKLETIGFDTKKDLL
                     ISVGDLVDRGTENVECLELITFPWFRAVRGNHEQMMIDGLSERGNVNHWLLNGGGWFF
                     NLDYDKEILAKALAHKADELPLIIELVSKGKKYVICHADYPCDKYEFGKPVDHQQVIW
                     NRERISNSQDGIVKEIKGAD"
BASE COUNT          201 a          119 c          153 g          135 t
ORIGIN      
        1 gcagggccat caaggcggag tatcaacaga aacttaaaaa actgcgaaac agcagaagtg
       61 aggttgcatg aatatctacg aaagaattga tggcagcaaa taccgaaata tttgggtagt
      121 tggcgatctg cacggatgct acacgaacct gatgaaaaaa ctggagacga taggattcga
      181 caccaaaaaa gacctgctta tctcggtggg cgatttggtt gatcgcggta cagagaacgt
      241 agaatgcctg gaattaatca cattcccctg gttcagagct gtacgtggaa accatgagca
      301 aatgatgatt gatggcttat cagagcgtgg aaacgtcaat cactggctgc ttaatggcgg
      361 tggctggttc tttaatctcg attacgacaa agaaattctg gctaaagctc ttgcccataa
      421 agcagatgaa cttccgttaa tcatcgaact ggtgagtaaa ggaaaaaaat atgtcatctg
      481 ccacgccgat tatccttgtg ataaatacga gtttggaaag ccagttgatc atcagcaggt
      541 aatctggaac cgcgaacgaa tcagcaactc acaagacggg atcgtgaaag aaatcaaagg
      601 cgcggaca
//
LOCUS       DAZFAB010000392          605 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZFAB010000392 DAZFAB010000000
VERSION     DAZFAB010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.397"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>605
                     /locus_tag="ACHJWA_005218"
     CDS             <1..>605
                     /locus_tag="ACHJWA_005218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO4311606.1"
                     /translation="KTDLIPEGVIRTDDERTHRYHYDSQHRLVHYTRTQYEEPLVESR
                     YLYDPLGRRVAKRVWRRERDLTGWMSLSRKPQVTWYGWDGDRLTTIQNDRTRIQTIYQ
                     PGSFTPLIRVETATGELAKTQRRSLADALQQSGGEDGGSVVFPPVLVQMLDRLESEIL
                     ADRVSEESRRWLASCGLTVAQMQSQMDPVYTPARKIHLYHC"
BASE COUNT          138 a          176 c          199 g           92 t
ORIGIN      
        1 aaaaccgacc tcatcccgga aggggttatc cgcacggatg atgagcggac tcaccggtac
       61 cattacgaca gtcagcaccg gctggtgcac tacacgcgga cacaatatga agagccgctg
      121 gtcgaaagcc gctatcttta cgacccgctg ggccgcaggg tggcaaaacg ggtgtggcga
      181 cgtgaacggg acctgacggg ctggatgtcg ctgtcacgga aaccgcaagt gacctggtac
      241 ggctgggacg gcgaccggct gaccacaata cagaacgaca gaacccgcat ccagacgatt
      301 tatcagccgg ggagcttcac gccactcatc agggttgaaa ccgccaccgg tgagctggcg
      361 aaaacgcagc gccgcagcct ggcggatgcg cttcagcagt ccggcggcga agacggtggc
      421 agtgtggtgt tcccgccggt gctggtgcag atgctcgacc ggctggaaag tgaaatcctg
      481 gctgaccggg tgagtgagga aagccgccgc tggctggcat cgtgcggcct gactgtggcg
      541 cagatgcaaa gccagatgga cccggtatac acgccggcgc gaaaaatcca cctgtaccac
      601 tgcga
//
LOCUS       DAZFAB010000393          605 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZFAB010000393 DAZFAB010000000
VERSION     DAZFAB010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.398"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>605
                     /locus_tag="ACHJWA_005219"
     CDS             <1..>605
                     /locus_tag="ACHJWA_005219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFO4311607.1"
                     /translation="QCQVIIGNTVSQAFQEVVSLLPGDMQPALPVGKPKLTLRRIGAG
                     ILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFFLPL
                     MVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYTYTV
                     IPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPL"
BASE COUNT          126 a          165 c          169 g          145 t
ORIGIN      
        1 accagtgtca ggtgattatt ggcaataccg tttcacaagc ctttcaggaa gtcgtcagcc
       61 tgctgccggg agatatgcag cccgccctgc ccgtgggtaa acccaaactc acgctgcgtc
      121 gcattggtgc ggggatcctc gatgcgctga tcggcaccat gtcaccgctg atcccggcga
      181 ttatcggcgg atcgatggtc aaactgctgg cgatgatcct cgagatgagc ggcgtgctga
      241 caaaaggatc gccgacctta accattctga atgtgattgg tgacggtgct ttcttcttcc
      301 tgccgctgat ggtcgcggct tcggccgcca tcaaatttaa aaccaatatg tcgctggcga
      361 ttgccatcgc gggtgtgctg gtacatccga gttttattga actgatggcg aaagcggccc
      421 agggtgaaca tgtcgaattt gccctgattc cagtcaccgc ggtgaaatac acctacacag
      481 tgatcccggc gctggtgatg acctggtgcc tgtcatatat cgaacgctgg gtggatcgca
      541 ttacaccggc ggtgaccaaa aacttcctca agccaatgct gattgtgttg attgccgcac
      601 cgctg
//
LOCUS       DAZFAB010000394          588 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZFAB010000394 DAZFAB010000000
VERSION     DAZFAB010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.399"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>588
                     /locus_tag="ACHJWA_005220"
     CDS             <1..>588
                     /locus_tag="ACHJWA_005220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO4311608.1"
                     /translation="GYINRSRATGVSKGPGQQEAVSRLAEELTGKKHTSPKTRSAGER
                     EEEQAREALLALEAELRTLEKHSGANEKISRQRRDLWKAESQYAVLKEAATKRQLSEQ
                     EKSLLAHKDETLEYKRQLAELGDKVEYQKRLNELAQQAVRFEEQQSAKQAAISAKARG
                     LTDRQAQRESEAQRLRDVYGDNPAALAKATSALKNT"
BASE COUNT          161 a          150 c          196 g           81 t
ORIGIN      
        1 gggtacatca accgctcgcg tgcaacaggc gtcagcaaag gtccggggca gcaggaagcc
       61 gtcagccgtc tggctgaaga gctgacaggt aaaaagcata catcaccgaa aacgcgctct
      121 gccggggaga gggaagagga gcaggcaaga gaggctctgc ttgcccttga agctgagctc
      181 aggacgctgg aaaaacacag cggtgcgaat gagaaaatca gccggcagcg ccgtgattta
      241 tggaaggcgg aaagtcagta tgcggtcctg aaagaggctg ccacgaaacg acagttatct
      301 gagcaggaaa aatccctgct ggcgcataaa gacgagacgc tggagtacaa acgccagctg
      361 gctgagctgg gcgacaaggt tgaataccag aaacgcctga atgagctggc acagcaggcg
      421 gtgcggtttg aagagcagca gagcgcgaag caggccgcca tcagcgcaaa agcccgcggt
      481 ctcactgacc gtcaggcgca gcgggagtct gaagcgcagc gtcttcggga cgtgtacggt
      541 gataatccgg ctgcgctggc gaaggccaca tcggcactga agaacacc
//
LOCUS       DAZFAB010000395          585 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZFAB010000395 DAZFAB010000000
VERSION     DAZFAB010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.400"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>585
                     /locus_tag="ACHJWA_005221"
     CDS             <1..>585
                     /locus_tag="ACHJWA_005221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HFO4311609.1"
                     /translation="NHSINMFREVSISGDIVSVKFYRNEKIECACDFMMAKDAQGYID
                     LSELDLTSCHFKGDVISKVSFISSNLQHVTFECKEIGDCNFTTAIVDNVIFKCRRLHN
                     VIFIKASGDYVDFSKNILDTVDFSQSQLTHSNFCECQIRNSNFDHCYLYASHFTRAEF
                     LTDKEISFIKSNLTAVMFDHVRISTGNFKDSVTQ"
BASE COUNT          196 a           82 c          105 g          202 t
ORIGIN      
        1 aaaatcattc aataaatatg tttcgtgaag tctcaatcag tggtgatatt gtaagcgtaa
       61 aattttatag aaatgaaaaa atagaatgcg cttgtgattt tatgatggct aaagatgcgc
      121 aggggtatat cgacctgtct gaattggatt taacaagttg tcattttaaa ggtgacgtta
      181 tttcgaaggt gtctttcata tcatcaaatc tacaacatgt aacattcgaa tgtaaagaaa
      241 ttggggattg caattttact actgcaatag ttgataatgt catatttaaa tgtcgacgtt
      301 tacacaatgt aatttttatc aaagcgagtg gtgattatgt cgattttagc aaaaatattc
      361 ttgatacagt tgacttctcg cagagtcaac ttactcatag taatttttgt gaatgtcaga
      421 ttagaaattc aaacttcgat cattgttatc tttatgcttc gcacttcacc agagcagaat
      481 ttcttactga caaagaaata tcatttatta aatcgaattt aacagctgtt atgtttgatc
      541 atgtgcgaat atcgacaggg aattttaaag acagcgttac acaac
//
LOCUS       DAZFAB010000396          582 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZFAB010000396 DAZFAB010000000
VERSION     DAZFAB010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.401"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(80..409)
                     /locus_tag="ACHJWA_005222"
                     /pseudo
     CDS             complement(80..409)
                     /locus_tag="ACHJWA_005222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416442.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(367..582)
                     /locus_tag="ACHJWA_005223"
                     /pseudo
     CDS             complement(367..582)
                     /locus_tag="ACHJWA_005223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140296.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          155 a          143 c          131 g          153 t
ORIGIN      
        1 gcgtctatgg acaaggcgtt tcctgctggt gataaataaa ggcgcagcac gtcgaatgca
       61 agaatgcgct gcgtctggct catggcaatt cattatcttc gcatagcgtc aatgacaaaa
      121 atcgactgtg tggatcaaat tgataatcag caaacggttc gcaatcaacg aaaccatgct
      181 tcaaataaag ttgatggcag gccgtaaatc cagcctgtgt acccgtttct aagctcaggc
      241 gatgaaggca tctgtcatgg gcgacctgca aaatgtggcg taaaatcaga ctggcgacac
      301 cccgacgtaa ataatttggc gcggtacgca ttgatttcag ttcgccatgt ttatcatcca
      361 gcaactttag cgcacccatt cctgcgagtt gttcgccttc ccatactgac ctgttgacct
      421 ggcttgttta atgacatccg atctcaccgc gtggggttat ggatggggca aactcactca
      481 atttctggtt taaaatggaa acctgattct gaaagttttc tgccacccac gatccgtata
      541 cgcgataaac cctctgatca tcagaatgtc tcatttgaca ca
//
LOCUS       DAZFAB010000397          581 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZFAB010000397 DAZFAB010000000
VERSION     DAZFAB010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.402"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            353..472
                     /locus_tag="ACHJWA_005224"
                     /pseudo
     CDS             353..472
                     /locus_tag="ACHJWA_005224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          167 a          137 c           91 g          186 t
ORIGIN      
        1 ttatgtaagg agtatttgat catgtataag caataaaaaa cagcttcagg taatgaggaa
       61 tatctcaatt tttaaacata aaatgcgaat tatttagtac aaaaagtgaa cttttgatga
      121 tctttcccta atctcacata ggatcagatc tcgtctgatc gttccccctg cgctcactac
      181 catggctctt taccacagca gccggggttg tttgggaccg acttccgaca gcagatcccg
      241 gtgggcctgc caccatctct tcacaagctt atgcttatta catgcaaaac gtcccgtttt
      301 gcatctttgt gcctacggca caccgatgat ctttccctgt gatctttccc tgaatctgcc
      361 cctgagaacc tgaatcaaac gtctgactcg cagaaccatc tgtttctcgc gctctgtaga
      421 acttcatgaa aaagtcatcg gtgcttttat cgaaaaatac atgttctact aattgaagtc
      481 atcatcaaaa aatttaatta atcgaatcat acctataacc ttttaatttt acttttttgt
      541 cttcaacatt aaacacaaaa acctcttgga caattctttt t
//
LOCUS       DAZFAB010000398          571 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZFAB010000398 DAZFAB010000000
VERSION     DAZFAB010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.403"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>571
                     /gene="espR3"
                     /locus_tag="ACHJWA_005225"
     CDS             <1..>571
                     /gene="espR3"
                     /locus_tag="ACHJWA_005225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector leucine-rich repeat protein EspR3"
                     /protein_id="HFO4311610.1"
                     /translation="NLLNMLSEFKLLREQCFRWGNYTLLFENYGAYDKTGSITIEKSQ
                     GEGTLPIRHKLEFISTNIAELLDKLTKITDARLCKGFSDWASSVKEGASNDLKENVDR
                     ALVRMFKCVKLHSNELNLSSLSLGSVPPLPEWIEMLSLVYNELDSIQVPESCKELELD
                     FNNLTEFPQVPDGITLISVNNNLISYIDS"
BASE COUNT          178 a           94 c          125 g          174 t
ORIGIN      
        1 gcaacttact gaatatgctg tctgaattta agttattgag agagcaatgt ttcaggtggg
       61 gtaattatac tctattgttt gaaaattatg gggcttatga taagacggga tctatcacga
      121 tagaaaaaag tcagggggag gggactttac ccattcggca taaattagag tttatatcga
      181 ccaatattgc agagttgctg gacaagttaa ccaaaattac agatgccagg ctttgcaaag
      241 gtttcagtga ctgggctagt tcagtcaaag aaggcgcatc gaatgacttg aaagaaaatg
      301 tggatagagc attggtgaga atgtttaaat gtgttaagct tcacagtaat gaacttaact
      361 tatcaagcct ttctttgggt tctgtgccgc ctcttcctga gtggattgaa atgcttagcc
      421 ttgtttataa tgaacttgat tcaatacagg tgcccgaatc gtgcaaagaa ttagaactcg
      481 atttcaataa ccttacagaa tttccacaag tacctgatgg aattaccctg atctccgtaa
      541 ataataacct gatatcgtat attgactcat t
//
LOCUS       DAZFAB010000399          570 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZFAB010000399 DAZFAB010000000
VERSION     DAZFAB010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.404"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(1..401)
                     /locus_tag="ACHJWA_005226"
                     /pseudo
     CDS             complement(1..401)
                     /locus_tag="ACHJWA_005226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
BASE COUNT          190 a          112 c           80 g          188 t
ORIGIN      
        1 aataatgcta ttttcaataa tgcctggcaa tgaatactgc caaacttcat tttttctttt
       61 acagcaaatt tgagacctaa aacttctatt tgctgattca atgttaacgt tctatcttca
      121 ggttcttgtg ctgttaattc tctgattgtt acaaccagat cagggtcggt agtgattata
      181 atctcaaaac tacctgtaag ctcgccataa ggaacgacta ttgtattttc tggtaactca
      241 gaaaaagcat cacttcttcc taacgaaaca tcattaccaa tgataacaag tagacgccca
      301 ttaagtattt catactcgca tccattctat ggtccgctca acaaacgaat ggcataagat
      361 tccaataagt cttgtgataa gaatttatca ttattttcca tgaataccac ccgctaattc
      421 cataagctct agtttagagt agtgtaacaa gacggaaatt tcatgaccaa attatgatga
      481 gacagaataa aaaaattata attttgagga ccattttaaa tgcctctatg cataacttta
      541 tttacaattt aaggtataaa ccctattccc
//
LOCUS       DAZFAB010000400          566 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZFAB010000400 DAZFAB010000000
VERSION     DAZFAB010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.405"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            2..532
                     /locus_tag="ACHJWA_005227"
     CDS             2..532
                     /locus_tag="ACHJWA_005227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO4311611.1"
                     /translation="MLAGTNEFIGEGDAYIPPHTGLPANSTDIAPPDIPAGFVAVFNS
                     NEASWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSPDGECQKWNGTSWAKDA
                     EAEKLFRVREAEETKNSLMQVASEHIAPLQDAVDLDIATEEEASLLAAWKTYRVLLNR
                     VDTTVAADVEWPVAPQ"
BASE COUNT          156 a          117 c          161 g          132 t
ORIGIN      
        1 tctgctggcc ggaactaatg aatttattgg tgaaggtgac gcatacattc cgcctcatac
       61 aggtctgcca gcaaacagta ccgatattgc accgccagat attccggctg gcttcgtggc
      121 tgttttcaac agtaatgagg catcgtggca tctcgttgaa gatcatcggg gtaaaacggt
      181 ttatgacgtg gcttccggcg acgcgttatt tatttctgaa ctcggtccgt taccggaaaa
      241 tgttacctgg ttgtcgccgg atggagagtg tcagaagtgg aacggcacat cctgggcgaa
      301 agatgcagaa gcagaaaaac tgtttcgggt acgggaggca gaagaaacaa aaaacagcct
      361 gatgcaggta gccagtgagc atattgcgcc acttcaggat gccgtagatt tggatattgc
      421 gacggaggaa gaggcatcgt tactggctgc atggaagaca tatcgggtat tgttgaatcg
      481 tgttgataca acagtagcag cggatgttga gtggccagtc gccccacaat aaaaagaaaa
      541 agccatcgac agaaatatcg atggct
//
LOCUS       DAZFAB010000401          562 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZFAB010000401 DAZFAB010000000
VERSION     DAZFAB010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.406"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>562
                     /locus_tag="ACHJWA_005228"
     CDS             <1..>562
                     /locus_tag="ACHJWA_005228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFO4311612.1"
                     /translation="DSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVG
                     EEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGT
                     IKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDN
                     IKMVVTLIHPIAMDDGLRFAIREGGRT"
BASE COUNT          136 a          140 c          155 g          131 t
ORIGIN      
        1 tggattccta cattccggaa ccagagcgtg cgattgacaa gccgttcctg ctgccgatcg
       61 aagacgtatt ctccatctcc ggtcgtggta ccgttgttac cggtcgtgta gaacgcggta
      121 tcatcaaagt tggtgaagaa gttgaaatcg ttggtatcaa agagactcag aagtctacct
      181 gtactggcgt tgaaatgttc cgcaaactgc tggacgaagg ccgtgctggt gagaacgtag
      241 gtgttctgct gcgtggtatc aaacgtgaag aaatcgaacg tggtcaggta ctggctaagc
      301 cgggcaccat caagccgcac accaagttcg aatctgaagt gtacattctg tccaaagatg
      361 aaggcggccg tcatactccg ttcttcaaag gctaccgtcc gcagttctac ttccgtacta
      421 ctgacgtgac tggtaccatc gaactgccgg aaggcgtaga gatggtaatg ccgggcgaca
      481 acatcaaaat ggttgttacc ctgatccacc cgatcgcgat ggacgacggt ctgcgtttcg
      541 caatccgtga aggcggccgt ac
//
LOCUS       DAZFAB010000402          555 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZFAB010000402 DAZFAB010000000
VERSION     DAZFAB010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.407"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..>555)
                     /locus_tag="ACHJWA_005229"
     CDS             complement(<1..>555)
                     /locus_tag="ACHJWA_005229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFO4311613.1"
                     /translation="QFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAISIASILE
                     NNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQSNIWPVSI
                     YYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDAMQSKSAE
                     RLCNEDFNGSYFNSGVMYINLREW"
BASE COUNT          190 a          110 c           78 g          177 t
ORIGIN      
        1 accattcccg taaattaata tacattacac cagagttaaa atagctaccg ttaaaatcct
       61 cattacacaa acgctcagcg cttttacttt gcatagcatc tacatcaatt accactgccc
      121 catattcatc tttaaactct aatgctatta actcgttcaa tgaaccctta cagacgatat
      181 cagcatcaag atataataat gaatccaatc gcgcagaaaa ataatcaaat gaaagcaaac
      241 gataataaat agatactggc cagatatttg attgaggtag cgccttcaat ggctcagaat
      301 caatatgata taatttaatt actgtttgat acttcgttgc taattgtgat aataattcaa
      361 tatactcttt gtctaagtaa tcagcgatta tgtgaaaggt aaatttgttt tgtttattat
      421 tttcaagaat tgaagcaatt gaaatcgcag ccccaacctg gtagttctca tcaatacccc
      481 atgagacatt aaacgtttcc cgttcatcaa gtttaacggg gcgcttgtcc agttcaatta
      541 tatctttaaa ttgag
//
LOCUS       DAZFAB010000403          552 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZFAB010000403 DAZFAB010000000
VERSION     DAZFAB010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.408"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..>552)
                     /locus_tag="ACHJWA_005230"
     CDS             complement(<1..>552)
                     /locus_tag="ACHJWA_005230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO4311614.1"
                     /translation="DSFRQKDADLQSQYQTGDISEDFYRQALAQNAQYLSERLKDQAV
                     FYAESDVQRADWQKGLQEGFSNWVDNASDYASQAAQLATEGISGMVNNITEMLNGNKV
                     EWRSWASSVLQEISKVLMNAAIVNGIKTAANGMSGAGGFLGSIGDWLGGAVANAKGGV
                     YTSANLSAYSNSIVDTPTYFAFA"
BASE COUNT          109 a          176 c          125 g          142 t
ORIGIN      
        1 tttgcaaacg caaagtacgt gggcgtgtcc acaatgctgt tgctgtacgc gctcaggttt
       61 gccgaggtat acacgccgcc ttttgcattg gccaccgctc cgcccagcca gtcaccaatg
      121 ctgccgagaa atcctcccgc accggacata ccgtttgccg ccgtcttaat tccgttgaca
      181 atcgcggcat tcataagaac ttttgatatt tcctgcagca ctgatgaggc ccagctgcgc
      241 cattccactt tatttccgtt cagcatctcc gtgatgttat tcaccatccc tgagataccc
      301 tccgtcgcca gctgtgctgc ctgtgaggcg taatcggacg cattatccac ccagttactg
      361 aatccctcct gcagcccttt ctgccagtcc gcacgctgca catccgattc ggcataaaag
      421 actgcctggt ccttaaggcg ttcgctcaga tactgcgcgt tctgtgccag agcctgtctg
      481 taaaaatcct cactgatatc cccggtctga tactgagact gaaggtccgc atccttctgg
      541 cggaagctgt cg
//
LOCUS       DAZFAB010000404          550 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZFAB010000404 DAZFAB010000000
VERSION     DAZFAB010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.409"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(379..>550)
                     /gene="grlA"
                     /locus_tag="ACHJWA_005231"
     CDS             complement(379..>550)
                     /gene="grlA"
                     /locus_tag="ACHJWA_005231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE transcriptional
                     regulator GrlA"
                     /protein_id="HFO4311615.1"
                     /translation="HYKRLEIFIYDVNLEAVPIESPGTTGPKRKTYRVGNGIVGQSNI
                     WNEMIMRRKKES"
BASE COUNT          164 a          121 c           86 g          179 t
ORIGIN      
        1 accatcagct ataccaatcc ccaggctgac tggggatttt ctatcggaaa taataaagct
       61 gctatagtga agtgctccca aagttgagca ccgaccactg gtagatttat ctacaggtgt
      121 ggggttattc cattaaccag gagtttatgc cctgaactat cttctgctgt atttaatatc
      181 atttccgttt atgaaacaat cagtatatct taacttagca ttagaaatct gatctttcca
      241 atcatctata gctcagagta tatttgtcag gaaatgacct tcaaacttta gccgagaaaa
      301 aggcttaccc tggaaaacaa aacccttaaa tatagcttta tttttattct tctataaaat
      361 atactcaaaa aattacgtct aactctcctt tttccgcctc atgatcattt cgttccagat
      421 attagactgt cccacaatac cattaccaac tcggtaggtt tttctttttg gtccggttgt
      481 tccaggactt tctatcggaa ccgcctcaag gttaacatca taaatgaaaa tctcaaggcg
      541 cttataatgc
//
LOCUS       DAZFAB010000405          546 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZFAB010000405 DAZFAB010000000
VERSION     DAZFAB010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.410"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            6..530
                     /gene="ybeT"
                     /locus_tag="ACHJWA_005232"
     CDS             6..530
                     /gene="ybeT"
                     /locus_tag="ACHJWA_005232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO4311616.1"
                     /translation="MFIVAAITCISQPKKTTLRDKAMVNYAFDYLSSPGSLPFTTAAT
                     ELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRAQSKLGWIYLK
                     GLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGSGINVNHYESQKWLKLAAKQH
                     YKNAERLLAGLPAH"
BASE COUNT          166 a          127 c          119 g          134 t
ORIGIN      
        1 tcacaatttt tatagttgca gcaattacct gtattagcca acccaagaaa acgacgttgc
       61 gtgataaagc catggtgaat tatgcctttg attatttaag ctcaccgggc agtcttccat
      121 tcaccacggc agccacggag ctttccgcga ttcatggtca ctcaacgtcg caatatcgcc
      181 ttggagaatt ttatcttcat ggtagcgacg gtaaaccact ggattataca caggcgagat
      241 actggtatga gcaatcagcg gaacaggaaa atccacgcgc gcaaagtaaa ctggggtgga
      301 tctacctcaa aggtctgggg gtcaaacccg acacccgtaa agcaattctc tggtataagg
      361 aagcagctga acaagggtat gctcatgctc aatatacttt aggtttgatc tacagaaatg
      421 gctcaggtat taatgttaac cattatgaat ctcaaaaatg gttaaaactg gccgccaaac
      481 aacattacaa aaatgcggaa agattacttg ccgggcttcc cgcacattaa cgtcgactgt
      541 cacccg
//
LOCUS       DAZFAB010000406          533 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZFAB010000406 DAZFAB010000000
VERSION     DAZFAB010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.411"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(246..524)
                     /locus_tag="ACHJWA_005233"
     CDS             complement(246..524)
                     /locus_tag="ACHJWA_005233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspO"
                     /protein_id="HFO4311617.1"
                     /translation="MPFSIKSIFSGHTWHQPEISRPIADKSSTKNCILDSTTCNVDGF
                     TVFNRRSCSFDMRPPGSADRTPQLRLSISEVAWMSKIIETETNNTNKS"
BASE COUNT          156 a           94 c          102 g          181 t
ORIGIN      
        1 ttttcgtgtc tttaagtgat accagatggt attgcgctaa atgtaccaat gggtatacca
       61 agagcactaa aaacaaaggg gcaaccactt tgtaacccct tgattatttg cagaagcgca
      121 gaaaatcgaa ctctggaacc ctttcggggc atcctttttc aagaatgaag aaaaaaataa
      181 ctaaaaaatc aagttgttag gaagttattc atggctagtg taccaactct tttctgggct
      241 aatagctatg atttgtttgt attatttgtc tctgtctcta ttatttttga catccatgcg
      301 acttctgata ttgataacct cagttgtgga gttctgtctg cgcttccggg aggacgcata
      361 tcaaaactac aggatcttct gttaaacact gtaaagccat ctacattgca tgttgttgag
      421 tctaagatac aatttttagt agatgattta tctgctatag gcctagatat ttcaggttga
      481 tgccaagtgt gtcctgaaaa aatacttttt attgaaaatg gcatttttat cct
//
LOCUS       DAZFAB010000407          531 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZFAB010000407 DAZFAB010000000
VERSION     DAZFAB010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.412"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..310)
                     /locus_tag="ACHJWA_005234"
     CDS             complement(<1..310)
                     /locus_tag="ACHJWA_005234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO4311618.1"
                     /translation="MSEINYQALREKAEKATKGSYIVGHTSVNQHGNLTGVFVCQKWK
                     GEPGGVIAECHVNCLVETDVQAYANAEFIAAFNPNVALALLDERERNQQYIKRRDQEN
                     E"
     gene            complement(307..528)
                     /locus_tag="ACHJWA_005235"
     CDS             complement(307..528)
                     /locus_tag="ACHJWA_005235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO4311619.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNYQTVSATHCCECGDPIDE
                     RRRLAVPGCRTCASCQEDLELISKQRGSK"
BASE COUNT          120 a          141 c          103 g          167 t
ORIGIN      
        1 cctcgttctc ctggtcgcgg cgtttgatgt attgctggtt tctttcccgt tcatccagaa
       61 gcgccagcgc aacatttgga ttaaaggcag caataaattc agcgtttgca taagcctgaa
      121 catctgtttc aaccaggcag ttaacatgac attccgcaat cacaccaccg ggttctcctt
      181 tccatttttg gcaaacaaaa actcctgtta aattgccgtg ctggttaaca gatgtatgcc
      241 ctacgatgta gcttccttta gttgctttct ctgccttttc acgcagtgcc tgataattaa
      301 tttcgctcac ttcgaacctc tctgtttact gataagctcc agatcctcct ggcaacttgc
      361 acaagtccga caacccggaa ctgccaggcg tcttcgttca tctatcggat cgccacactc
      421 acaacaatga gttgcggata cagtctggta gttcaggcga cgcattttta ttgctgtatt
      481 gcgctgtaat tcttcaattt ctgatgctga atcaatgatg tctgccatct t
//
LOCUS       DAZFAB010000408          531 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZFAB010000408 DAZFAB010000000
VERSION     DAZFAB010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.413"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..310)
                     /locus_tag="ACHJWA_005236"
     CDS             complement(<1..310)
                     /locus_tag="ACHJWA_005236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO4311620.1"
                     /translation="MSEINYQALREKAEKATKGSYIVGHTSVNQHGNLTGVFVCQKWK
                     GEPGGVIAECHVNCLIESDAQAYANAEFIAEANPATVLELLDERERNQQYIKRRDQEN
                     E"
     gene            complement(307..528)
                     /locus_tag="ACHJWA_005237"
     CDS             complement(307..528)
                     /locus_tag="ACHJWA_005237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO4311621.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNYQTVSATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEDLELISKQRGSK"
BASE COUNT          112 a          142 c          105 g          172 t
ORIGIN      
        1 cctcgttctc ctggtcgcgg cgtttgatgt attgctggtt tctttcccgt tcatccagca
       61 gttccagcac ggtagccggg ttagcctctg ctatgaattc agcgtttgca taagcctgag
      121 catctgattc aatcaggcag ttaacatgac attccgcaat cacgccaccg ggttctcctt
      181 tccatttttg gcaaacaaaa actcctgtta aattgccgtg ctggttaaca gatgtatgcc
      241 ctacgatgta gcttccttta gttgcttttt ctgccttttc acgcagtgcc tgataattaa
      301 tttcgctcac ttcgaacctc tctgtttact gataagctcc agatcctcct ggcaacttgc
      361 acaagtccga caaccctgaa ctgccaggcg tcttcgttca tctatcggat cgccacactc
      421 acaacaatga gttgcggata cagtctggta gttcaggcga cgcattttta ttgctgtatt
      481 gcgctgtaat tcttcaattt ctgatgctga atcaatgata tctgccatct t
//
LOCUS       DAZFAB010000409          530 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZFAB010000409 DAZFAB010000000
VERSION     DAZFAB010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.414"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>530
                     /locus_tag="ACHJWA_005238"
     CDS             <1..>530
                     /locus_tag="ACHJWA_005238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HFO4311622.1"
                     /translation="ASRNVTKCHGESLRGHIDVTTEDLASALDVTEDVIDSILHAMQG
                     RVLDGDLISGWEKRQVMKEDNGNVSQTAKSPAERKRAQRERERLREQNTDCHDESRRV
                     THMSRQVTTDTDTDKELNPTHNARMRESAPASESNGAPLQTAEPEYPDGLSEPIGKFP
                     MTGVWRPSLDFRQRAA"
BASE COUNT          147 a          123 c          161 g           99 t
ORIGIN      
        1 gtgcgtcacg aaatgtcacg aaatgtcacg gcgagtcact acgtggtcac attgatgtca
       61 cgacggaaga tttagcaagt gcgcttgatg tgacggaaga cgtgattgat tcaattttgc
      121 atgcaatgca ggggcgagtt ctggatggcg atcttatttc cggatgggaa aaacgccagg
      181 tgatgaagga ggataacggt aatgtttcgc aaaccgcaaa atccccggca gagcgcaaga
      241 gagcgcagcg ggagcgggaa agactgcggg agcagaacac tgattgtcac gatgagtcac
      301 gacgcgtcac gcatatgtca cgacaagtca cgacagatac agatacagat aaagaattaa
      361 accccacaca taacgcgcgc atgcgcgaga gtgctccagc cagtgagtcg aatggcgcgc
      421 cgttgcagac agcagaacct gaatacccgg atggcctgag cgaaccgatc gggaaatttc
      481 cgatgactgg tgtctggcgg ccgtcgctgg attttcgaca gcgggctgca
//
LOCUS       DAZFAB010000410          524 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZFAB010000410 DAZFAB010000000
VERSION     DAZFAB010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.415"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..272
                     /locus_tag="ACHJWA_005239"
     CDS             <1..272
                     /locus_tag="ACHJWA_005239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311623.1"
                     /translation="NIIEIGYNIINTDLVIGEKIHFRTLSNRSLYFNLTHPPLIATIK
                     DDVFCTLHDYYNHNNETKSTIDNYISKIKDNHNTPWLLNNENVQE"
BASE COUNT          213 a          108 c           65 g          138 t
ORIGIN      
        1 acaatataat tgaaataggc tacaatataa taaacaccga tctggtcatt ggcgaaaaaa
       61 ttcatttcag gacactaagc aatcgttctt tatattttaa cctcacccac cccccattaa
      121 tcgccacaat aaaagatgat gtgttttgca ctcttcatga ttactataac cacaataatg
      181 aaacgaaatc cactattgac aattacatat caaaaataaa ggataatcat aataccccat
      241 ggttattaaa caacgaaaat gttcaggaat aaaaaaaaag agacaattcc cgagataaaa
      301 caaaagagca taaacatatg aaccggaaat tctatttcaa aaatatcctg tggggaatgt
      361 gccccattat aatatccata ttgcatctga tatatgacac accaacagaa gcatcgataa
      421 aagttgactt atttctgata ctcagtggcc tggcccttcc ttgttcaata tacctgacaa
      481 aaacaatcac actaaaatac acatccatat cattctggaa aaaa
//
LOCUS       DAZFAB010000411          523 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZFAB010000411 DAZFAB010000000
VERSION     DAZFAB010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.416"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>523
                     /locus_tag="ACHJWA_005240"
     CDS             <1..>523
                     /locus_tag="ACHJWA_005240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4225 domain-containing protein"
                     /protein_id="HFO4311624.1"
                     /translation="KARLINELKMESETARKEYQLFRQGNYDKYITTDIFEEHGLIKY
                     VNLSFDIVASVGQVVGGVGALKFGKVVHSNRIKGIGVTLVAHGANNFYESLSPLFFNE
                     YDSGPIRELYRVIAKKMGGDINSADYAYSIVDFSITAYGGYSGMKIVPKYNRLIRPSL
                     GNRPGTGRLFHYTS"
BASE COUNT          181 a           75 c          109 g          158 t
ORIGIN      
        1 aaaagcacga cttatcaatg aattaaaaat ggagtcagaa acagcaagaa aagaatatca
       61 acttttcaga caaggaaatt atgataaata tatcactact gatattttcg aagaacatgg
      121 tttgataaaa tatgtgaact taagtttcga tattgttgca tctgtaggac aggtcgtagg
      181 cggggttggc gcgttgaaat ttggtaaggt agtgcattca aaccgaataa aaggtatagg
      241 cgtcacatta gttgctcatg gggcgaataa tttttatgaa agcttatcac ctttattttt
      301 taacgagtat gattcaggtc caatacgtga actctacaga gtaatagcaa aaaaaatggg
      361 aggtgatatt aattctgctg attatgcata cagcatcgtg gacttttcga ttactgcata
      421 tggtggatat tcaggcatga aaatcgtacc taagtataat agattgattc gtccttcatt
      481 aggaaataga cctggtacag gacgattatt tcattacaca tca
//
LOCUS       DAZFAB010000412          523 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZFAB010000412 DAZFAB010000000
VERSION     DAZFAB010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.417"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>523
                     /locus_tag="ACHJWA_005241"
     CDS             <1..>523
                     /locus_tag="ACHJWA_005241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin B"
                     /protein_id="HFO4311625.1"
                     /translation="RLSIECHFSNNEREFIKMGYYNGCDVILESDTIQKIKSFAKNIT
                     IQTADGVMANWDDKLNTLSVYSIDMIAWRDKNKTAKEPLPVDVIQLTNWRMHNTCSLF
                     YDKYQVDIEANKLTYTVLFPDTELPVQLNYTSIIYGNHGAKYTFLNSGSKTIDIHILD
                     KNSDYDTFDFRNII"
BASE COUNT          194 a           73 c           89 g          167 t
ORIGIN      
        1 cgcctatcta ttgaatgcca ctttagtaat aatgaaagag agtttataaa gatgggctac
       61 tataatggat gtgatgtgat tttagaaagt gacactatac aaaaaataaa atcatttgca
      121 aaaaatataa caatacagac agcggatgga gttatggcaa attgggatga taaactaaat
      181 actctgtctg tttattcaat agatatgatt gcatggaggg ataaaaataa aactgcaaaa
      241 gaaccgttac cagttgatgt aatccagtta actaattgga ggatgcataa cacatgctct
      301 ttattttatg ataaatatca agttgatatt gaagccaata agttaacgta tacagtctta
      361 tttcctgata cggagttacc cgttcagctc aattatacca gtattattta tggtaatcat
      421 ggggcaaaat acaccttcct caattcagga agtaaaacta tagatattca cattcttgat
      481 aaaaatagtg attatgatac ttttgacttc cgtaatatta ttt
//
LOCUS       DAZFAB010000413          522 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZFAB010000413 DAZFAB010000000
VERSION     DAZFAB010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.418"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            189..>522
                     /gene="grlR"
                     /locus_tag="ACHJWA_005242"
     CDS             189..>522
                     /gene="grlR"
                     /locus_tag="ACHJWA_005242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE GrlA-binding
                     negative regulator GrlR"
                     /protein_id="HFO4311626.1"
                     /translation="MIMKDGIYSIIFISNEDSCGEGILIKNGNMITGGDIASVYQGVL
                     SEDEDIILHVHRYNYEIPSVLNIEQDYQLVIPKKVLSNDNNLTLHCHVRGNEKLFVDV
                     YAKFIEPLV"
BASE COUNT          185 a           68 c          103 g          166 t
ORIGIN      
        1 ttgttactca atattattaa tcagaaatta catatgttaa ccagggaaac agcaggttga
       61 aacatgagta tatttaatga tatattacat tgcaatctgg agaaaaagaa aggtctccat
      121 tattcttgat attgcttatg gatagaacaa attgaaagga gtgaggttag tatgaaactg
      181 agtgagttat gattatgaag gatggcatct atagcattat atttattagc aatgaagact
      241 cctgtgggga aggtatactg attaaaaatg gaaatatgat cactggcggc gatattgctt
      301 ctgtgtatca gggggtcctc tctgaagatg aggacatcat acttcatgtc catcgatata
      361 attacgaaat tccctcggtg ctaaacattg aacaagatta tcaattagtt atccctaaaa
      421 aagtactgag taatgataat aatctcacat tacattgcca tgtaagagga aatgaaaaat
      481 tgtttgttga tgtttatgcc aaatttatag aaccattagt ta
//
LOCUS       DAZFAB010000414          521 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZFAB010000414 DAZFAB010000000
VERSION     DAZFAB010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.419"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..95
                     /locus_tag="ACHJWA_005243"
     CDS             <1..95
                     /locus_tag="ACHJWA_005243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311627.1"
                     /translation="RSSLKENTETVEMNSKVWGRLKKIITFFKK"
     gene            complement(171..473)
                     /gene="bamE"
                     /locus_tag="ACHJWA_005244"
     CDS             complement(171..473)
                     /gene="bamE"
                     /locus_tag="ACHJWA_005244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFO4311628.1"
                     /translation="MKNETQESVKTKIVKGKTTKQDVLASFGEPDSRSLIDGEEQWSY
                     TMYNSQSKATSFIPVVGLLAGGADSQTKSLTVSFKGEKVSTYIFNAGTSNVKTGIF"
BASE COUNT          145 a          100 c          110 g          166 t
ORIGIN      
        1 taagatcctc tttaaaggaa aatactgaga ctgtagaaat gaacagtaaa gtttggggga
       61 ggctgaaaaa gataatcact ttctttaaaa agtgatttat gtttctgtaa aaagcccgta
      121 ggtatcaact gcgggctaaa ttttcccttg atgctggttt tgtcttttat ctaaaaaatg
      181 ccagtcttca cgttgcttgt tccagcatta aagatgtatg tgctgacttt ttcgcctttg
      241 aaagaaactg tcagagattt agtttgtgag tctgcgccac ctgcaagcag tcccacaacg
      301 gggatgaaag aggttgcttt ggactggctg ttatacatag tgtatgacca ttgttcttca
      361 ccatcgatca aagaacggct gtcaggttca ccgaacgatg ctaacacgtc ctgttttgta
      421 gttttgcctt taacaatttt ggttttcaca ctttcctgcg tctcattttt caatgactgg
      481 ttgcctactg aagaacaacc tgccaaggtc agaaaaacga a
//
LOCUS       DAZFAB010000415          506 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZFAB010000415 DAZFAB010000000
VERSION     DAZFAB010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.420"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>506
                     /locus_tag="ACHJWA_005245"
     CDS             <1..>506
                     /locus_tag="ACHJWA_005245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311629.1"
                     /translation="SGLWGKISQWSGINNQGREDYRKRDFAPDSPEIKFSDRREALEL
                     MTQIESTVTSLHREAEAQFRPELEKIVSGIETGFRGTALYATENIAGRINARLEDEGF
                     TVKISFPAVSQLQTRLAVKINLSALMEERTETVTRRRRQSGVWGTVCRWFGTSDLGWE
                     NYDEDVSR"
BASE COUNT          133 a          122 c          153 g           98 t
ORIGIN      
        1 gtccggatta tgggggaaaa tttctcaatg gtccggtatc aacaaccagg gccgggagga
       61 ttacaggaaa cgagattttg ccccggacag ccccgaaata aaattcagtg atcgcaggga
      121 agcccttgaa ctgatgacgc aaatcgaatc gaccgtgacc agcctgcacc gtgaggctga
      181 agcacagttc cggcctgagc tggagaaaat cgtcagcggg attgaaacag gttttcgtgg
      241 cacggccctg tacgccacag aaaacattgc cggtcgcatc aatgcccgcc tggaggatga
      301 gggctttacc gtaaaaatca gttttccggc agtcagccag ttacagaccc ggctcgcggt
      361 aaaaataaat ctgagtgcgc ttatggagga aagaacggag accgtcaccc gtcgccgtcg
      421 gcagagtggc gtatggggaa ccgtttgtcg atggtttggc accagtgacc tgggctggga
      481 aaactatgac gaggatgtga gtcgca
//
LOCUS       DAZFAB010000416          504 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZFAB010000416 DAZFAB010000000
VERSION     DAZFAB010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.421"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..302
                     /locus_tag="ACHJWA_005246"
     CDS             <1..302
                     /locus_tag="ACHJWA_005246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFO4311630.1"
                     /translation="LLDILRHKALTQMAQESGGSATVRLNTLDWLGGQGREQADNEWH
                     DAINWLGDWCSEEQHPVIWSTTQAAEHLPVRMPRLCSAERLSESMVDEIFQKGAA"
     gene            299..>504
                     /locus_tag="ACHJWA_005247"
     CDS             299..>504
                     /locus_tag="ACHJWA_005247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFO4311631.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGASIG
                     AFNGAIIAASTDLSEAAVRLEALW"
BASE COUNT          114 a          116 c          170 g          104 t
ORIGIN      
        1 agctactgga tattctcaga cataaagcat taacgcagat ggcacaggag agtggcggta
       61 gtgcaaccgt gcgtctgaac actctggact ggcttggtgg tcagggccgg gagcaggcag
      121 ataacgagtg gcatgacgcg attaactggc tgggagactg gtgcagtgaa gagcagcatc
      181 cggtgatctg gtcaaccacg caggctgcag aacacttacc ggttcgcatg cctcgcctct
      241 gctctgcaga acgcctctct gaaagtatgg tcgatgaaat atttcagaag ggggctgcat
      301 gagtacagaa atgaaaacgg ggctggtgct gtccggtggc ggggcggtgg gcgcttatca
      361 ggcgggagtg gttaaggcac tggcagagtg tggtacacag atcagcatgg tttcaggggc
      421 cagcattggc gcattcaatg gtgccattat cgcggcctct accgatctgt cagaagctgc
      481 cgtacgcctg gaggcgctct ggga
//
LOCUS       DAZFAB010000417          504 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZFAB010000417 DAZFAB010000000
VERSION     DAZFAB010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.422"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(75..422)
                     /gene="tnpB"
                     /locus_tag="ACHJWA_005248"
     CDS             complement(75..422)
                     /gene="tnpB"
                     /locus_tag="ACHJWA_005248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO4311632.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
BASE COUNT          112 a          141 c          140 g          111 t
ORIGIN      
        1 tgtcgtcaga agagatgtcg ttcataagcc cgtattttac cgggcttatt ctgtgacaac
       61 caggataaag agatttacag catggtcagg gaggtcagca gccgcttagg ctgtcgccag
      121 tcgatacctt ccagcagcat cgccagctgc gcctgcgtaa ggaacacttt gccatcacgg
      181 gctgacggcc aggcgaagcg cccacgctcc agccgtttgg tcaggaggca cagtccgtca
      241 ccggtggacc acagcagttt aacctgactg ccgctgcggc cccggaaaat gaaaacatgg
      301 ccggacatgg gatcgtcttt cagcgccgtt tgtactttcg cagccaggcc gttgaagcca
      361 tttctcatat cggtgatacc ggcaaccagc caaattttgg tcccggaagg taacgggatc
      421 atcgcttcag ttcctgtatc agcagagtca ggagcttttc gctgacattg ccattgaagc
      481 ggagcgtccc gtgccggaac gtta
//
LOCUS       DAZFAB010000418          498 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZFAB010000418 DAZFAB010000000
VERSION     DAZFAB010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.423"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>498
                     /gene="wzx"
                     /locus_tag="ACHJWA_005249"
     CDS             <1..>498
                     /gene="wzx"
                     /locus_tag="ACHJWA_005249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O157 family O-antigen flippase"
                     /protein_id="HFO4311633.1"
                     /translation="KYDTSSALGREQERYRIISLSVISSLISKILSLLSLILTVSLTL
                     PYLGQERFGVWMTITSLGAALTFLDLGIGNALTNRIAHSFACGKNLKMSRQISGGLTL
                     LAGLSFVITAICYITSGMIDWQLVIKGINENVYAELQHSIKVFVIIFGLGIYSNGVQK
                     VYMGI"
BASE COUNT          158 a           68 c          102 g          170 t
ORIGIN      
        1 aaaaatatga tacatcaagt gctttaggta gagaacagga aaggtacagg attatatcct
       61 tgtctgttat ttcaagtttg attagtaaaa tactctcact actttctctt atattaactg
      121 taagtttaac tttaccttat ttaggacaag agagatttgg tgtatggatg actattacca
      181 gtcttggtgc tgctctgaca tttttggact taggtatagg aaatgcatta acaaacagga
      241 tcgcacattc atttgcgtgt ggcaaaaatt taaagatgag tcggcaaatt agtggtgggc
      301 tcactttgct ggctggatta tcgtttgtca taactgcaat atgctatatt acttctggca
      361 tgattgattg gcaactagta ataaaaggta taaacgagaa tgtgtatgca gagttacaac
      421 actcaattaa agtctttgta atcatatttg gacttggaat ttattcaaat ggtgtgcaaa
      481 aagtttatat gggaatac
//
LOCUS       DAZFAB010000419          480 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZFAB010000419 DAZFAB010000000
VERSION     DAZFAB010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.424"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..>480)
                     /locus_tag="ACHJWA_005250"
     CDS             complement(<1..>480)
                     /locus_tag="ACHJWA_005250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO4311634.1"
                     /translation="AVTWQGRQYQAYPIDGSGFEMNGKGSSARPSLTVSNLFGLVTGM
                     AEDLQSLVGATVVRRRVYARFLDAVNFVAGNPEADPEQELTDRWVVEQMSSLTAMTAS
                     FVLATPTETDGALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCS"
BASE COUNT           92 a          180 c          117 g           91 t
ORIGIN      
        1 ttactgcatc tgtccttccg gatatccgtg gtggggttgt cgaactcatc cgccactgcc
       61 ggaccgttat acccgcattc atcgccccgg taatcccaca tacaggtgtt cgccagcatg
      121 atgcgaccgg gaaacagcgc cccgtccgtc tccgtcggtg tcgccagcac aaacgaggcc
      181 gtcatggccg tcagcgatga catctgctcc accacccacc ggtccgtcag ctcctgctca
      241 gggtctgcct caggattgcc tgccacaaaa ttcaccgcat ccagaaaacg ggcatacacc
      301 cggcggcgga ccaccgtggc ccccaccagg ctctgcaggt cctccgccat cccggtgacc
      361 agaccaaaca gattcgacac cgtcagcgac ggtctggcac tgctgccctt cccgttcatc
      421 tcaaagccac tgccgtcaat cgggtatgcc tgatattgcc gcccctgcca ggtaaccgcc
//
LOCUS       DAZFAB010000420          480 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZFAB010000420 DAZFAB010000000
VERSION     DAZFAB010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.425"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..>480)
                     /locus_tag="ACHJWA_005251"
     CDS             complement(<1..>480)
                     /locus_tag="ACHJWA_005251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO4311635.1"
                     /translation="PVTWQGREYQAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGM
                     AEDLQSLVGATVVRRRVYARFLDAVNFVAGNPEADPEQELTDRWVVEQMSELTAMTAS
                     FVLATPTETDGALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCS"
BASE COUNT           93 a          179 c          118 g           90 t
ORIGIN      
        1 ttgctgcatc tgtccttacg gatatccgtg gtggggttgt cgaactcatc cgccaccgca
       61 ggaccgttat acccgcattc atctccccgg taatcccaca tacaggtgtt cgccagcatg
      121 atgcgaccag gaaacagcgc cccgtccgtc tccgtcggtg ttgccagcac aaacgaggcc
      181 gtcatggccg tcagctctga catctgctcc accacccacc ggtcagtcag ctcctgctcc
      241 gggtcggcct ccggattgcc tgccacaaag ttcaccgcat ccagaaaacg cgcatacacc
      301 cggcggcgga ccaccgtggc acccaccagg ctctgcaaat cctccgccat cccggtgaca
      361 agaccgaaca gattggacac cgtcagcgac gggcgggcac tgctgccctt tccgttcatc
      421 tcaaagccgc tgccctcaat cgggtacgcc tgatattcac gcccctgcca ggtcaccggc
//
LOCUS       DAZFAB010000421          478 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZFAB010000421 DAZFAB010000000
VERSION     DAZFAB010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.426"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..>478)
                     /locus_tag="ACHJWA_005252"
     CDS             complement(<1..>478)
                     /locus_tag="ACHJWA_005252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3491 domain-containing protein"
                     /protein_id="HFO4311636.1"
                     /translation="TWETYGDIVVSPEDRISHLELDGFNYFSQPELDTPISDSFSYLY
                     DNFQIVDSDVHIKLLHLNRETKQITPHRIILKRYFIDSFAKTSITDREKNIYPVICDS
                     PDHFTSDIYRHPFRIVLGNKTLYPSEELVKFISTSKEYLSNMDVINNVIVPQKTTKK"
BASE COUNT          150 a           73 c           79 g          176 t
ORIGIN      
        1 ttttctttgt tgttttttga ggaacaataa cattgttaat cacatccata ttgcttaaat
       61 attcttttga ggtgcttatg aattttacaa gttcttctga tggatacaat gtcttattcc
      121 cgagaactat tctaaatgga tgcctatata tgtcgcttgt gaagtgatca ggtgaatcac
      181 aaatcacagg atatatattc ttttctctgt cagtgatact tgttttagca aatgagtcta
      241 taaagtatct tttaagaata atacgatgtg gtgttatttg ctttgtttct ctgttgagat
      301 gaagaagctt aatatgcaca tcactatcaa cgatctgaaa attatcatat aaatatgaaa
      361 atgagtcaga tattggagta tctaactcgg gttgtgaaaa ataattaaac ccatctaact
      421 ccagatgaga aattctatct tcaggtgata caacgatatc accatacgtt tcccatgt
//
LOCUS       DAZFAB010000422          474 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZFAB010000422 DAZFAB010000000
VERSION     DAZFAB010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.427"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            3..431
                     /gene="etgA"
                     /locus_tag="ACHJWA_005253"
     CDS             3..431
                     /gene="etgA"
                     /locus_tag="ACHJWA_005253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE muramidase EtgA"
                     /protein_id="HFO4311637.1"
                     /translation="MSNYCYASDCFEITGKAYNIDPLILKAIAWNESKCKSGIKSKTN
                     KNGTYDIGIMQINSSHLDLLSKFNISEDDLLNDACINISVAGYILASNIKSRGNTWDA
                     VGAYNAGYFNTPNAVELRRQYAMKIYKTYNKLKNNEQIID"
BASE COUNT          195 a           65 c           76 g          138 t
ORIGIN      
        1 tcatttcaaa ttactgttat gcttccgatt gttttgaaat tacaggaaaa gcatataaca
       61 tagatccatt aatattaaaa gcaattgcat ggaatgaatc aaaatgcaaa agcggaataa
      121 aaagcaaaac aaacaaaaac ggaacgtatg atataggtat aatgcaaatt aattcatctc
      181 atttagattt attaagcaaa tttaatatat ctgaagatga tttattgaat gatgcctgta
      241 taaacatatc tgttgctggt tatatcttag cctctaacat aaaatccaga gggaatacat
      301 gggatgcagt tggtgcgtat aatgcaggtt actttaatac accaaatgca gtagaactca
      361 gaaggcaata cgcaatgaaa atatataaaa catataataa attaaagaat aatgagcaaa
      421 ttatcgatta ataatcaagg tctatcgcaa tacgaatcaa ttgtgccaca ggtt
//
LOCUS       DAZFAB010000423          470 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZFAB010000423 DAZFAB010000000
VERSION     DAZFAB010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.428"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..335
                     /locus_tag="ACHJWA_005254"
     CDS             <1..335
                     /locus_tag="ACHJWA_005254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO4311638.1"
                     /translation="KREIAVDADYDHDRIVWVDEDGNEANKRHSRRLELLHENFREPP
                     EKWRRVAVKDIDEFVTCCFTEQGCKDYLAVNGHNLRLPFIYVKSGFRNAEYIGIRNWL
                     AGIRIKGE"
     gene            337..>470
                     /locus_tag="ACHJWA_005255"
     CDS             337..>470
                     /locus_tag="ACHJWA_005255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311639.1"
                     /translation="MALTHRELCQIAYKFLKRNGFKVCFHDRFIAVTSTGEQPDAMGF
                     "
BASE COUNT          134 a           98 c          116 g          122 t
ORIGIN      
        1 aaaagcgcga aatcgctgtt gatgctgatt atgaccatga ccggattgtc tgggttgacg
       61 aagatggcaa tgaagccaat aaacgccata gtcgtcgtct cgagctactt catgaaaact
      121 ttcgagagcc accagaaaaa tggcggcgcg ttgctgtgaa agatattgat gaattcgtta
      181 cctgctgttt caccgaacag ggttgtaaag actacctggc agtcaatggt cacaatcttc
      241 gcttgccatt tatatatgta aaaagcggtt tcaggaacgc tgaatatatc ggcataagaa
      301 actggcttgc tggcattcgc atcaaaggag agtgatatgg cgttaacaca ccgcgaactc
      361 tgtcagattg cgtacaagtt ccttaagcgc aacggtttca aggtttgttt tcatgaccgc
      421 tttatagctg taaccagtac cggagaacag ccagatgcta tgggattcag
//
LOCUS       DAZFAB010000424          470 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZFAB010000424 DAZFAB010000000
VERSION     DAZFAB010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.429"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..454
                     /locus_tag="ACHJWA_005256"
     CDS             <1..454
                     /locus_tag="ACHJWA_005256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO4311640.1"
                     /translation="IEADIRGMPAEQRLAERQRKTKPLLKSLESWLREKMKTLSRHSE
                     LAKAFAYALNQWPALTYYANDGWVEIDNNIAENALRAVSLGRKNFLFFGSDHGGERGA
                     LLYSLIGTCKLNDVDPESYLRHVLGVIADWPVNRVSELLPWRIALPAE"
BASE COUNT          118 a          118 c          141 g           93 t
ORIGIN      
        1 catagaggcg gatataaggg gaatgccggc agagcagcgg cttgctgaac gtcagcgaaa
       61 aacgaaaccg ttgttgaaat ccctggaaag ctggttgcgt gaaaagatga agaccctgtc
      121 gcgacactca gagttggcga aggcgttcgc gtacgcactt aaccagtggc cggcactgac
      181 gtactatgcg aacgatggct gggtggaaat cgacaacaac atcgctgaaa atgccctgcg
      241 ggcggtcagt ctgggtcgta aaaacttcct gttcttcggc tctgatcatg gtggtgagcg
      301 gggagcgcta ctgtacagcc tgatcgggac gtgcaaactg aatgacgtgg atccagaaag
      361 ctaccttcgc catgtgcttg gcgtcatagc agactggccg gtcaaccggg tcagcgaact
      421 gcttccgtgg cgcatagcac tgccagctga ataacacatc cccgtcaata
//
LOCUS       DAZFAB010000425          469 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZFAB010000425 DAZFAB010000000
VERSION     DAZFAB010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.430"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            191..>469
                     /locus_tag="ACHJWA_005257"
     CDS             191..>469
                     /locus_tag="ACHJWA_005257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HFO4311641.1"
                     /translation="MDCSKCNGYATMLLNMVQGSDPVNLLELHGFLEHFAYYVSFGKF
                     NAGHQRYNAFKKFVSEISEISANDINMTIKTGQSRHENVISINMNDAIP"
BASE COUNT          146 a           82 c           96 g          145 t
ORIGIN      
        1 atcgctcatt tcagcttatt gatagaaaga ttgattgcca gtctgtttag agccgtacag
       61 cagcaaactt tcataaccag gttgatcctg aatttgcagt acgaaaagtt aaaaatgagc
      121 gatttctgta agactcccca ttttgccagg cttatgattc gcagatcatt caagtgcaaa
      181 ggagcgatgt atggattgtt caaaatgcaa tggttatgcg acgatgttat taaatatggt
      241 gcagggttct gacccggtta atttactgga attacatggt ttccttgaac atttcgcata
      301 ttatgtttca tttggcaaat ttaatgctgg ccaccagcgc tataatgcat ttaaaaaatt
      361 cgtcagtgag atttcggaga tcagcgcaaa tgatatcaat atgacgatta aaaccgggca
      421 atccagacat gaaaatgtga tctctataaa tatgaatgat gcaattcct
//
LOCUS       DAZFAB010000426          467 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZFAB010000426 DAZFAB010000000
VERSION     DAZFAB010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.431"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>467
                     /locus_tag="ACHJWA_005258"
     CDS             <1..>467
                     /locus_tag="ACHJWA_005258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311642.1"
                     /translation="IKNPIVFLGYSLTDRNVKKLLSDFSSQLPREDGRKSAERIILIE
                     YKENEQEVVPKQITDQQLQITYTSVKTDNYKQIYDEISAVDEGLSPYDVLRYQRAIKT
                     LIINEGEKGHLDTLLVSPSDLDRLEESVKQGKNLVVALGDKKYVFTQVKEINY"
BASE COUNT          182 a           67 c           80 g          138 t
ORIGIN      
        1 tgattaaaaa tccaatagtt tttcttggat attctttaac tgacaggaat gtaaagaaac
       61 ttctttctga tttttcctca caactaccta gagaagatgg tcgaaaatca gctgaaagaa
      121 taattttgat tgaatataaa gagaatgaac aagaagtagt accaaaacag attaccgatc
      181 aacagctaca aattacatat acatctgtta aaacagataa ctataaacag atctatgatg
      241 agatcagtgc tgttgatgaa gggctctcac cttatgatgt tttaaggtat caaagagcca
      301 ttaaaacact tatcattaat gagggtgaaa aagggcactt agatactcta ttggtttcac
      361 cttctgactt agacagactt gaagaaagtg ttaaacaagg aaaaaattta gttgttgctc
      421 taggcgataa aaagtatgta ttcacacaag ttaaagaaat aaattat
//
LOCUS       DAZFAB010000427          462 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZFAB010000427 DAZFAB010000000
VERSION     DAZFAB010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.432"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..228
                     /locus_tag="ACHJWA_005259"
     CDS             <1..228
                     /locus_tag="ACHJWA_005259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO4311643.1"
                     /translation="PLTRAADGSLGVRAVGSMNGSAGLVYSPVYHIAIQNDGANGQIG
                     PEAAGSLVQLIDQRVQAVMLSMRRDGGMLSG"
     gene            221..>462
                     /locus_tag="ACHJWA_005260"
     CDS             221..>462
                     /locus_tag="ACHJWA_005260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4311644.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVVRVRFGDGYEQRRPTGLNPQL
                     KTFQAVFRVTDESTRRWLDEFLSWHGGYRAFLWRPP"
BASE COUNT           85 a          112 c          169 g           96 t
ORIGIN      
        1 cccctgaccc gggcggcgga tggctcgctg ggcgtacgcg ccgtgggcag tatgaacggc
       61 agtgctggtc tggtgtattc cccggtctac cacatcgcca ttcagaatga cggggctaac
      121 ggacagatag ggccggaggc ggcaggcagt cttgtgcagc tgattgacca gcgggtgcag
      181 gcggtgatgc tgtccatgcg acgtgacgga ggaatgctga gtggctgaga taaaaacgct
      241 gcatctggtc ccgcgtgaag ggatgcaggt gagtgagaag ccgtcggtgg tgagggtgcg
      301 gtttggtgac ggttatgaac agcgccgccc cacagggctg aatcctcaac tgaagacgtt
      361 tcaggcggtg ttccgggtga cggatgagtc aacccggcgc tggctggatg aatttttatc
      421 ctggcatggt ggttaccgtg cctttttgtg gcgaccgccg aa
//
LOCUS       DAZFAB010000428          461 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZFAB010000428 DAZFAB010000000
VERSION     DAZFAB010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.433"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>461
                     /gene="espX1"
                     /locus_tag="ACHJWA_005261"
     CDS             <1..>461
                     /gene="espX1"
                     /locus_tag="ACHJWA_005261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HFO4311645.1"
                     /translation="NITIEKSNLSYGYYFGCVLSNISCFESDLSNTIFSNGEINNLFI
                     KKSNIFGTSFTNTMIKNLRCEDIMPGRWTTQLVNKHLGYRYTGVFKTLASIDDKPSRF
                     EILIPLVQTLVRDNVKLNNDVYKELKKFMHDYDKTSPEMRKYLQSINESMFL"
BASE COUNT          172 a           85 c           74 g          130 t
ORIGIN      
        1 aatataacaa tagaaaagag caacctttca tatgggtatt attttggttg tgtattatcc
       61 aatatatcat gttttgaaag cgacttatca aacaccatct tttcaaatgg agaaataaac
      121 aacctcttca ttaaaaaaag caacatattt ggaacatcgt tcactaatac catgataaaa
      181 aatttgcgct gtgaagatat tatgcctggc cgatggacca ctcaacttgt caataagcac
      241 ttaggttaca gatacacagg agtattcaaa accctggcaa gtattgatga caaaccgtcc
      301 cgtttcgaaa ttttgatacc attggtacaa actttagttc gcgacaatgt aaagctaaat
      361 aatgatgtgt ataaagaact gaaaaagttt atgcacgatt acgacaaaac atccccagaa
      421 atgagaaaat accttcagag catcaacgag tctatgttcc t
//
LOCUS       DAZFAB010000429          440 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZFAB010000429 DAZFAB010000000
VERSION     DAZFAB010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.434"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>440
                     /locus_tag="ACHJWA_005262"
     CDS             <1..>440
                     /locus_tag="ACHJWA_005262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HFO4311646.1"
                     /translation="IDKILVNKNRNYEILYGRDHVIYINTNSLDEAVWVKQALEKNQP
                     GKPVRVINPDDESIRIFSWLADNFPDLQYFKLQLLDASNPRLTVSKQRNAITQQLIDN
                     LIKGLLQTMPYASNISIAVLDDNVLESQAIETLSAIGLSYEKYK"
BASE COUNT          161 a           71 c           80 g          128 t
ORIGIN      
        1 atatcgataa gattcttgta aacaaaaaca ggaattatga aattttatat ggtagagatc
       61 atgtcatcta tatcaatacc aatagtttgg atgaagcagt ctgggtcaaa caagcactgg
      121 aaaaaaatca accaggaaag ccagtacggg tgataaatcc tgatgatgaa tcgatacgaa
      181 ttttttcatg gcttgctgat aatttccctg atttacaata ttttaaactt cagctattag
      241 atgccagtaa tcccagactg accgtgagta agcaacggaa tgctatcaca cagcaactaa
      301 tcgacaatct tattaaaggg ttactacaaa ctatgccata tgccagcaat ataagtattg
      361 cggtattaga tgataatgta ttggaaagtc aggctattga aacattgtct gcaataggtc
      421 tttcttatga aaaatataaa
//
LOCUS       DAZFAB010000430          438 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZFAB010000430 DAZFAB010000000
VERSION     DAZFAB010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.435"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>438
                     /gene="espR1"
                     /locus_tag="ACHJWA_005263"
     CDS             <1..>438
                     /gene="espR1"
                     /locus_tag="ACHJWA_005263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector leucine-rich repeat protein EspR1"
                     /protein_id="HFO4311647.1"
                     /translation="NKLASLHNALFPNLNTLLINNNLLSEIKMFYSNFCKVQTLNAAN
                     NQLEKINLHFLTYLSSIKSLRLDNNKITRIDTENTSDIRSLFPIIKKSESLNFLNISG
                     ENNCPTIQLMLFNLFSPALKLNTGLAILSPGAFEDHSDGLDVD"
BASE COUNT          149 a           86 c           61 g          142 t
ORIGIN      
        1 taataaacta gcaagtttac acaatgcctt atttcccaac ttaaatacgt tacttatcaa
       61 caacaacttg ctttctgaaa ttaaaatgtt ttatagcaac ttctgcaaag ttcagacatt
      121 aaacgctgct aacaatcagt tggaaaaaat aaaccttcat ttcctgactt atctttcatc
      181 tatcaaaagt ttaaggctgg acaataataa aataactcgc attgatactg agaacacatc
      241 cgatattaga agtttattcc ccataataaa gaagagcgaa agcttaaatt ttttaaatat
      301 ttctggcgag aacaattgcc ctactatcca gctcatgtta tttaatttgt tttccccagc
      361 acttaagctt aatactggcc tggcaattct ttcgcctggt gcatttgaag atcactctga
      421 cggattagat gtggataa
//
LOCUS       DAZFAB010000431          430 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZFAB010000431 DAZFAB010000000
VERSION     DAZFAB010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.436"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>430
                     /locus_tag="ACHJWA_005264"
     CDS             <1..>430
                     /locus_tag="ACHJWA_005264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HFO4311648.1"
                     /translation="GVINSPNLEGPVFENCYFDDCKFNNIQLYETNDDTVESGNKKPI
                     KGMFKGCFISKCKIENYRCETSKIYTVTQPDSIQEKLGSYLFMQSFVQDCIIQGGCCP
                     GSSILLSHFYNCNIIGLDAHGMDFLANSFNKSNYDEIRGP"
BASE COUNT          142 a           62 c           85 g          141 t
ORIGIN      
        1 caggagtgat aaacagccct aaccttgaag gtccggtttt tgaaaattgc tatttcgatg
       61 attgtaaatt caacaacata cagctttatg agactaatga tgatactgtg gaatcaggaa
      121 ataaaaaacc aataaaaggt atgtttaaag gttgttttat ttctaaatgc aaaattgaaa
      181 attatagatg tgagacgtct aaaatctaca ccgttacaca acctgatagt attcaagaaa
      241 aattaggttc ttatctcttt atgcagtctt tcgttcagga ttgtataatc cagggaggat
      301 gttgtcctgg ctcgagtata cttctgagtc atttttataa ctgtaatata atagggcttg
      361 atgctcatgg aatggatttt ttggcaaatt cgttcaacaa gtctaattat gatgaaatca
      421 ggggtccgga
//
LOCUS       DAZFAB010000432          421 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZFAB010000432 DAZFAB010000000
VERSION     DAZFAB010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.437"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            1..332
                     /locus_tag="ACHJWA_005265"
                     /pseudo
     CDS             1..332
                     /locus_tag="ACHJWA_005265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696189.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            329..>421
                     /locus_tag="ACHJWA_005266"
     CDS             329..>421
                     /locus_tag="ACHJWA_005266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valyl-tRNA synthetase"
                     /protein_id="HFO4311649.1"
                     /translation="MTTITDKELIKEIKERIGSLDVRDNIERRAY"
BASE COUNT          129 a           68 c          113 g          111 t
ORIGIN      
        1 cttgttggca gatagaaaaa agcgttttcg taaaaatccg tctcttggaa tgggcgactg
       61 gcgattcttt attagtgagc cgggaattat ttcaattgag gatttaccac ctggctgggg
      121 attacttcac gttgttaacg gaagagtacg gaaagtacat gggtggccca agggtaattg
      181 ctgttggggt aatcctgacg ataagccatt tactggaaat aagtgaatgc gattacatgt
      241 tgtctgcatt aaggcgcatg gagttgagag ggcaccttaa tgaaatatat gacggtgtaa
      301 ttgttaataa gaaagaagga aacgcggcat gaccactatt accgacaaag aactgattaa
      361 agaaatcaaa gagcgcatag gcagcttgga cgttcgagac aatattgagc gccgtgctta
      421 t
//
LOCUS       DAZFAB010000433          418 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZFAB010000433 DAZFAB010000000
VERSION     DAZFAB010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.438"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..>418)
                     /locus_tag="ACHJWA_005267"
     CDS             complement(<1..>418)
                     /locus_tag="ACHJWA_005267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TcdA/TcdB catalytic glycosyltransferase
                     domain-containing protein"
                     /protein_id="HFO4311650.1"
                     /translation="KVDIEPFIHAVWVAGAPPDNTFSYITAFLNTYKDYTYLLWIDPN
                     AFGAAKFSGILKNIAMNYAIMRLRRTNPHLAEEMNEVILKIQNIQNETIEFKETRERL
                     KELENRYKSLTSETKEKFNVFFLESMIGMQDNYFTYC"
BASE COUNT          125 a           75 c           54 g          164 t
ORIGIN      
        1 acaatatgta aaatagttgt cttgcatacc tatcattgac tcaagaaaaa aaacattgaa
       61 tttttcttta gtttcactcg ttaatgattt atatctattt tcaagttcct tcaatctttc
      121 tcttgtttcc ttaaattcta tagtttcatt ttgtatgttt tgtattttta atatcacttc
      181 attcatttct tccgctaaat gtggattcgt tcttctcaaa cgcataattg cataattcat
      241 cgctatattt tttaaaatcc cactaaactt ggccgcacca aaggcattag gatcaatcca
      301 gagcaggtag gtataatctt tatatgtatt taaaaatgcc gttatatatg aaaatgtatt
      361 atcaggaggt gcgccagcaa cccatactgc atgtataaat ggctctatgt ctacttta
//
LOCUS       DAZFAB010000434          418 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZFAB010000434 DAZFAB010000000
VERSION     DAZFAB010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.439"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>418
                     /locus_tag="ACHJWA_005268"
     CDS             <1..>418
                     /locus_tag="ACHJWA_005268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4311651.1"
                     /translation="SFFRLSHRPSWRYLGIGEEEARAFSREVEAAWKEFAEDDCCCID
                     VERKRTFTMMIREGVAMHAFNGELFVQATWDTRPSRLFRTQFRMVSPKRISNPNNTSD
                     SRNCRAGVQINDSGAALGYYVSEDGYPGWMPQKWTW"
BASE COUNT           90 a          109 c          131 g           88 t
ORIGIN      
        1 ggtctttttt ccggctcagt catcgcccaa gctggcgcta tctgggcatc ggggaggaag
       61 aagcccgtgc cttttcccgc gaggttgaag cggcatggaa agagtttgcc gaagatgact
      121 gttgctgcat tgacgttgag cgaaaacgca cgtttaccat gatgattcgg gaaggtgtgg
      181 ccatgcacgc ctttaacggt gaactgttcg ttcaggccac ctgggatacc cgtccctcgc
      241 gactgttccg gacacagttc cggatggtca gcccgaagcg catcagcaac ccgaacaata
      301 ccagcgacag ccggaactgc cgtgccggtg tgcagattaa tgacagcggt gcggcgctgg
      361 gatattacgt cagcgaggac gggtatcctg gctggatgcc gcagaaatgg acatggat
//
LOCUS       DAZFAB010000435          416 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZFAB010000435 DAZFAB010000000
VERSION     DAZFAB010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.440"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>416
                     /gene="wzx"
                     /locus_tag="ACHJWA_005269"
     CDS             <1..>416
                     /gene="wzx"
                     /locus_tag="ACHJWA_005269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O157 family O-antigen flippase"
                     /protein_id="HFO4311652.1"
                     /translation="TWSGDNFIISITLGVTYVAVFSITQRLFQISTVPLTIYNIPLWA
                     AYADAHARNDTQFIKKTLRTSLKIVGISSFLLAFILVVFGSEVVNIWTEGKIQVPRTF
                     IIAYALWSVIDAFSNTFASFLNGLNIVKQQMLAVVT"
BASE COUNT          124 a           65 c           76 g          151 t
ORIGIN      
        1 acatggagtg gtgataactt tataatatct ataacattgg gtgttactta tgttgctgtt
       61 tttagcatta cacagagatt atttcaaata tctacggtcc ctcttacgat ttataacatc
      121 ccgttatggg ctgcttatgc agatgctcat gcacgcaatg atactcaatt tataaaaaag
      181 acgctcagaa catcattgaa aatagtgggt atttcatcat tcttattggc cttcatatta
      241 gtagtgttcg gtagtgaagt cgttaatatt tggacagaag gaaagattca ggtacctcga
      301 acattcataa tagcttatgc tttatggtct gttattgatg ctttttcgaa tacatttgca
      361 agctttttaa atggtttgaa catagttaaa caacaaatgc ttgctgttgt aacatt
//
LOCUS       DAZFAB010000436          412 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZFAB010000436 DAZFAB010000000
VERSION     DAZFAB010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.441"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>412
                     /locus_tag="ACHJWA_005270"
     CDS             <1..>412
                     /locus_tag="ACHJWA_005270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFO4311653.1"
                     /translation="GETVRYRYDDAYSELPATTTDATGSTRQMTWSRYGQLLAFTDCS
                     GYQTRYEYDRFGQMTAVHREEGISLYRRYDNRGRLISVKDAQGHETRYEYNAAGDLTA
                     VITPDGNRSETQYDAWGKAVSTTQGGLTRSMEYDL"
BASE COUNT           97 a          123 c          133 g           59 t
ORIGIN      
        1 gcggggagac agtacgctac cgctacgatg atgcgtacag tgagttaccg gcgacgacaa
       61 cagatgcgac gggcagcacc cggcagatga cctggagccg ctacggtcag ttgctggcgt
      121 tcaccgactg ctcgggctac cagacccgct atgaatacga ccgcttcggc cagatgacgg
      181 cggtccaccg cgaggaaggc atcagccttt accgccgcta tgacaaccgt ggccggttaa
      241 tctcggtgaa agacgcacag ggccatgaaa cgcggtatga gtacaacgcc gcaggcgacc
      301 tgactgccgt tatcaccccg gacggcaacc ggagcgagac acagtacgat gcgtggggaa
      361 aggcggtcag caccacgcag ggcgggctga cgcgcagtat ggaatacgac ct
//
LOCUS       DAZFAB010000437          412 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZFAB010000437 DAZFAB010000000
VERSION     DAZFAB010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.442"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>412
                     /locus_tag="ACHJWA_005271"
     CDS             <1..>412
                     /locus_tag="ACHJWA_005271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFO4311654.1"
                     /translation="GDVIRYAYDNPHSELPATTTDATGSTRQMTWSRYGQLLAFTDCS
                     GYQTRYEYDRFGQMTAVHREEGISRYRRYDNRGRLTSVKDAQGHETRYEYNAAGDLTA
                     VITPDGNRSETQYDAWGKAVSTTQGGLTRSMEYDL"
BASE COUNT           97 a          117 c          131 g           67 t
ORIGIN      
        1 gtggggacgt catccggtat gcttatgata atccgcacag tgaattaccg gccacgacaa
       61 cagatgcgac gggcagcacc cggcagatga cctggagccg ctacgggcag ttgctggcgt
      121 tcaccgactg ctcgggctac cagacccgtt atgaatacga ccgctttggt cagatgacgg
      181 cggtccaccg tgaggaaggt atcagccgtt accgccgcta tgacaaccgt ggccggttaa
      241 cctcggtgaa agacgcacag ggccatgaaa cgcggtatga gtacaacgcc gcaggcgacc
      301 tgactgccgt tatcactccg gacggcaacc ggagcgagac acagtacgat gcgtggggaa
      361 aagcggtcag caccacgcag ggcgggctga cgcgcagtat ggagtatgac ct
//
LOCUS       DAZFAB010000438          406 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZFAB010000438 DAZFAB010000000
VERSION     DAZFAB010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.443"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..243
                     /locus_tag="ACHJWA_005272"
     CDS             <1..243
                     /locus_tag="ACHJWA_005272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFO4311655.1"
                     /translation="AEEQVEKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRW
                     QLDLSALLASLQEEDGAATNKKTLAEYARALSGEDE"
     gene            240..>406
                     /locus_tag="ACHJWA_005273"
     CDS             240..>406
                     /locus_tag="ACHJWA_005273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFO4311656.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTTTSVSDLK
                     KYIAELEVQTG"
BASE COUNT          113 a           95 c          129 g           69 t
ORIGIN      
        1 gctgaagagc aggtcgaaaa atgggtggat ggcaggaaaa aaatactgtg ggacagcaaa
       61 aagcgacgca atgaggcgct cgactgcttc gtttatgcgc tggcggcgct gcgcatcagt
      121 atttcccgct ggcagctgga tctcagtgca ctgctggcga gcctgcagga agaggatggt
      181 gcagcaacca acaagaaaac actggcagaa tacgcccgtg ccttatccgg agaggatgaa
      241 tgacgcgaca ggaagaactt gccgctgccc gtgcggcact gcatgacctg atgacaggaa
      301 aacgggtggc aacggtacag aaagacggac ggcgagtgga gtttacgacc acttccgtgt
      361 ctgacctgaa aaaatacatt gctgagctgg aagtgcagac cggcat
//
LOCUS       DAZFAB010000439          403 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZFAB010000439 DAZFAB010000000
VERSION     DAZFAB010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.444"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>403
                     /locus_tag="ACHJWA_005274"
     CDS             <1..>403
                     /locus_tag="ACHJWA_005274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4765 family protein"
                     /protein_id="HFO4311657.1"
                     /translation="GSKVQLEVYNLLSEEKIFEHKIMNNWTRSIKNILTTYLFMSSGA
                     VTARNVQTFSPTINNESRIRLPRALPVGHPYPEEHKASGFSPFMMGGLSGDILPEILT
                     GNGPSIFFNGKHNNQHDGAFGKIIDFTQNGN"
BASE COUNT          145 a           60 c           83 g          115 t
ORIGIN      
        1 aaggaagcaa agttcaattg gaggtatata atttattatc cgaagaaaag atatttgaac
       61 ataaaattat gaataattgg acaaggagca taaaaaatat attgacgaca tatttgttta
      121 tgtcatcagg agcggtgaca gccagaaatg ttcaaacctt ttctccaaca ataaataatg
      181 agtcaaggat tcgattgccg agagcattgc cagtaggcca tccatatcct gaggaacata
      241 aggcttctgg tttctcccct tttatgatgg gggggctgag tggtgatatt cttccggaaa
      301 ttttaacggg gaatggacca tctatatttt ttaacggaaa acataataac caacatgatg
      361 gagcttttgg aaaaataata gattttaccc aaaatggaaa taa
//
LOCUS       DAZFAB010000440          399 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZFAB010000440 DAZFAB010000000
VERSION     DAZFAB010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.446"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>399
                     /locus_tag="ACHJWA_005275"
     CDS             <1..>399
                     /locus_tag="ACHJWA_005275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HFO4311658.1"
                     /translation="QSINDYFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLK
                     DQLSESIKELHQHGIVSGDPHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHY
                     NIKNELKDFGYTYLIFKKKIKKVIRDVKVKL"
BASE COUNT          154 a           52 c           70 g          123 t
ORIGIN      
        1 ccaatcaatt aatgactatt ttttgctagc tgaacgtaaa acattaaatt tcgctcatta
       61 ttatattatg ctcattgagt atattgaagg tgtcgggtta aatgagtatt tggaaatttc
      121 tgaagatctg aaggatcaat tatcagaatc gataaaagag ttgcatcagc atggaattgt
      181 gtctggcgat cctcataagg gaaattttat agtatccgaa aaaggtttaa gactaattga
      241 cctttcaggt aaaaaaacaa ccgctgtttt aaaagcaaaa gatcgcattg atcttgaacg
      301 gcactataat ataaaaaatg aattgaaaga ctttggctat acatatctta tattcaaaaa
      361 gaaaattaaa aaagtaatca gggatgtaaa agtcaaact
//
LOCUS       DAZFAB010000441          398 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZFAB010000441 DAZFAB010000000
VERSION     DAZFAB010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.447"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..>398)
                     /locus_tag="ACHJWA_005276"
     CDS             complement(<1..>398)
                     /locus_tag="ACHJWA_005276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFO4311659.1"
                     /translation="KGDAYANPLSDDDWQRLAKVKDLTPGEMTAEPYDDNYLDDEDAD
                     WTATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFESGDVRAYKIRFPNGTVDVFRGWV
                     SSIGKAVTAKEVITRTVKVTNVGKPSVAEE"
BASE COUNT           71 a          137 c           86 g          104 t
ORIGIN      
        1 ttcttccgcc acggaaggtt tgcccacgtt agtgactttc accgtgcggg tgatcacttc
       61 tttcgccgtc acggccttac cgatactgct gacccagcca cggaacacat ccaccgtgcc
      121 atttgggaaa cggattttat aggcccgcac atccccgctt tcaaaccagc ctataagccc
      181 tttctgacct tcttctcccg gtttccaggc cagcgtaaaa ctggtatctc ctgcagactt
      241 ctgcccctgc ccggtcgcgg tccagtccgc gtcttcatca tccaggtagt tatcatcgta
      301 gggttctgcc gtcatctcgc ccggcgtcag atccttcacc ttagccagtc gctgccagtc
      361 atcgtctgac aacgggtttg cataagcatc acccttgc
//
LOCUS       DAZFAB010000442          389 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZFAB010000442 DAZFAB010000000
VERSION     DAZFAB010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.448"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..167
                     /gene="ninE"
                     /locus_tag="ACHJWA_005277"
     CDS             <1..167
                     /gene="ninE"
                     /locus_tag="ACHJWA_005277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO4311660.1"
                     /translation="TPLIRVMNGHIYKVPNRRKRKPELKPSEIPTLLGYTASLVDKKW
                     LRLAARRNHG"
     gene            160..>389
                     /locus_tag="ACHJWA_005278"
     CDS             160..>389
                     /locus_tag="ACHJWA_005278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFO4311661.1"
                     /translation="MADLRKAARSRECQVRIPGVCNGNPETSVLAHIRLTGLCGTGTK
                     PPDLIATIACSACHDEIDRRTHFVDAAYAKECA"
BASE COUNT          111 a          102 c           94 g           82 t
ORIGIN      
        1 ctacaccgct tattcgtgtc atgaacggac acatctacaa agtaccaaat cgtcgtaagc
       61 gtaaacctga gctgaagcca tccgaaatac caacactgct cggatatacc gccagcctgg
      121 ttgataaaaa atggttgcga ctggcagcaa ggaggaatca tggctgattt gagaaaagca
      181 gcgcgtagtc gggaatgcca agtaagaatc cctggcgtat gtaatggcaa ccctgaaacg
      241 tctgtactgg cacatatccg gctgactgga ttgtgcggca ccggtaccaa accgccagac
      301 ttgattgcca ccattgcatg ttctgcctgc cacgacgaaa tcgaccgccg cacacatttt
      361 gtcgatgctg catatgcaaa agaatgcgc
//
LOCUS       DAZFAB010000443          386 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZFAB010000443 DAZFAB010000000
VERSION     DAZFAB010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.449"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..>386)
                     /locus_tag="ACHJWA_005279"
     CDS             complement(<1..>386)
                     /locus_tag="ACHJWA_005279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HFO4311662.1"
                     /translation="VIQTPFPFENNNSHTGVVTEPILGKLIGQGSTAEIFEDMNDSSA
                     LYKKYDLVGNQHNEVLEMARQESALFNTFYGDDASVVIQYGGDVYLRMLRVPGIPLSD
                     IDTADIPDNLESLYLQLICKLNELSI"
BASE COUNT          126 a           83 c           65 g          112 t
ORIGIN      
        1 tatactcaac tcattcaatt tacatatcaa ctgcagataa aggctctcta gattatcagg
       61 aatatcagct gtatcaatat cactaagggg aatccctggc acgcgtagca ttcggagata
      121 cacatcaccg ccatactgta taacaactga tgcgtcatcg ccataaaaag tattaaaaag
      181 ggcagattct tgcctagcca tttccagaac ttcattgtgc tggttaccaa caagatcata
      241 ctttttatat aaagcagacg aatcattcat atcttcaaag atttctgctg ttgaaccttg
      301 ccctattaac ttaccgagaa taggctccgt tactacgcca gtatgagaat tattattctc
      361 aaatgggaaa ggtgtttgaa taacag
//
LOCUS       DAZFAB010000444          378 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZFAB010000444 DAZFAB010000000
VERSION     DAZFAB010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.450"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..286
                     /locus_tag="ACHJWA_005280"
     CDS             <1..286
                     /locus_tag="ACHJWA_005280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311663.1"
                     /translation="FSTLLTINDNFLRAKAEIASTIIKFLEARITNLSYNDILKYQQE
                     FQKECYKQVKSFTTLSRYNKIQTWAEMSEYQFEVFQYETLNPKKCHARLI"
BASE COUNT          152 a           69 c           54 g          103 t
ORIGIN      
        1 attttcaaca ttattaacca ttaatgacaa ctttttacga gcaaaagcgg aaattgcttc
       61 gacaataatt aaatttttag aggccaggat aaccaatctt tcatacaacg acatattgaa
      121 atatcagcaa gaattccaaa aagaatgtta taagcaagtt aaatccttta cgacactctc
      181 tcgatacaat aaaattcaaa catgggcaga aatgtctgaa tatcaattcg aagtatttca
      241 atacgagacg cttaacccca aaaaatgtca cgcacgcctt atttaaagag gccacttcct
      301 aatgaaaaag atataaatta tggggtggaa atagaaatac catctggcaa aagaattcgc
      361 ttatcaaatc attatcag
//
LOCUS       DAZFAB010000445          368 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZFAB010000445 DAZFAB010000000
VERSION     DAZFAB010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.451"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..94
                     /locus_tag="ACHJWA_005281"
     CDS             <1..94
                     /locus_tag="ACHJWA_005281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311664.1"
                     /translation="YGDANINHYYRNLEIKNSHPKNRASSKRVS"
     gene            complement(182..>368)
                     /locus_tag="ACHJWA_005282"
     CDS             complement(182..>368)
                     /locus_tag="ACHJWA_005282"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311665.1"
                     /translation="ILKRNSYKEKYNYVIYWQIELTGGRNLRVRKVSNNNPLHQQTSN
                     RVPRYAGNTRRLNWRVV"
BASE COUNT          118 a           78 c           57 g          115 t
ORIGIN      
        1 atatggcgat gcaaatatca atcattatta tagaaactta gaaataaaaa actcacaccc
       61 caaaaataga gcatcaagca aaagagtaag ttaacatcat tgttaattaa ttcgaccagt
      121 cagcaaatat gctgactggt cgaacatagt ttgctaaaaa gtcatcactg actgatgtca
      181 gtcacaccac ccgccagttc aatcttcggg tatttccagc gtatctcggc acgcgattgc
      241 tggtctgttg atgtagtgga ttgttgttgc tgacttttct taccctcaga tttctgcccc
      301 ctgtcaactc aatctgccaa taaataacat aattatattt ttccttatat gaattccttt
      361 ttaaaata
//
LOCUS       DAZFAB010000446          366 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZFAB010000446 DAZFAB010000000
VERSION     DAZFAB010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.452"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>366
                     /locus_tag="ACHJWA_005283"
     CDS             <1..>366
                     /locus_tag="ACHJWA_005283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO4311666.1"
                     /translation="LFADVMNARLEQLDKAATPVEKIWDDVKTWTSDAWAWIGDHTLG
                     ALSLITDVVAGTVEQVKLLLVQGDLALAEFIQSAWETTKNVPGVGALFGELAEENRVF
                     IEKTKRDELALRKSIAERDA"
BASE COUNT           95 a           74 c          116 g           81 t
ORIGIN      
        1 cctgtttgcg gatgtcatga atgcacgtct ggagcaactt gataaagcgg ccacgccggt
       61 ggaaaaaatc tgggacgatg ttaaaacctg gacttctgac gcatgggcat ggataggtga
      121 tcatacactg ggggcactca gtctgatcac tgacgtggtg gccggaaccg ttgaacaagt
      181 gaagctgctg cttgtgcagg gggatctggc gctggctgaa tttattcagt cagcctggga
      241 aacgacaaag aatgtgcccg gcgttggtgc gttgtttggt gaactggcag aagagaaccg
      301 cgtatttatt gagaaaacaa aacgcgatga actggcgctg agaaaatcca ttgcggaacg
      361 ggatgc
//
LOCUS       DAZFAB010000447          359 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZFAB010000447 DAZFAB010000000
VERSION     DAZFAB010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.453"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            2..>359
                     /locus_tag="ACHJWA_005284"
     CDS             2..>359
                     /locus_tag="ACHJWA_005284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HFO4311667.1"
                     /translation="MYDAMNKGRMLAKGDLIHYLNAGDSVIGDIYKNIKEPCLIKVGL
                     FENDKLLGFSSITHSNTGYCHQGVIFPKNHSEYDLRYKICADYKLIQEVFPEGLRSLS
                     LITSGYVKYDMGGVSSK"
BASE COUNT          120 a           48 c           76 g          115 t
ORIGIN      
        1 gatatatgat gcgatgaata agggccgaat gttggccaaa ggcgacttaa tacattattt
       61 aaacgccggc gatagcgtaa ttggagatat atataaaaat atcaaagagc catgtttgat
      121 taaagttggc cttttcgaaa atgataaact tctgggattt tcttctataa cccattcaaa
      181 tacagggtat tgtcatcaag gggtgatttt cccaaagaat cattcagaat atgatctaag
      241 gtataaaata tgtgctgatt ataagcttat tcaagaggtg tttcctgaag ggttaagatc
      301 tctatctttg attacttcgg gttatgtaaa atatgatatg gggggagtat cttcaaaaa
//
LOCUS       DAZFAB010000448          359 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZFAB010000448 DAZFAB010000000
VERSION     DAZFAB010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.454"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            87..359
                     /locus_tag="ACHJWA_005285"
                     /pseudo
     CDS             87..359
                     /locus_tag="ACHJWA_005285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310182.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
BASE COUNT          111 a           49 c           92 g          107 t
ORIGIN      
        1 ttgtcatgta cggtggatgt ttgttatgac tccctgtgtt tggaatgaat atttaataac
       61 aggtgtctgg aaatataggg gcaaatctac tggataggct attggggcgt gagaatcgaa
      121 tggagagaag ggctgtggct ctggaaaggc aattaaatgg aggtgtcgat tttttaagga
      181 gtgttaataa ctattttcag agtgtcatgg cagaacacag agaaaataaa acaagtaata
      241 aaatattaat ggaaaaaata aattcctgtg tatttggaac ggattctaat cacttttctt
      301 gcccggagtc atttttgaca tgcccgataa cgctggacac acctgcgaat ggagtgttc
//
LOCUS       DAZFAB010000449          352 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZFAB010000449 DAZFAB010000000
VERSION     DAZFAB010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.455"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..209
                     /locus_tag="ACHJWA_005286"
     CDS             <1..209
                     /locus_tag="ACHJWA_005286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311668.1"
                     /translation="NAQKVINAEKYNEWVKKFSEQIFKITGDENAAKNELEPWTPEGV
                     DPNYCWWDVDPVDAANEAMSYHND"
BASE COUNT          127 a           58 c           91 g           76 t
ORIGIN      
        1 caaacgcaca gaaagttatt aacgctgaaa aatataacga gtgggtgaaa aagttctcag
       61 agcagatttt taaaattact ggcgacgaga atgcggcaaa aaatgaatta gagccgtgga
      121 cacctgaagg tgtcgaccca aattattgct ggtgggatgt tgatccagtt gatgctgcaa
      181 atgaagctat gagttatcac aacgattaat gtcaggaggc cgcccgaaag ggcggtaatg
      241 aaaagtgact gaattaacca aagagaaatt aatcgaagaa gccaaattaa aaatagcgat
      301 tgcgaaatgc taccccaatt cagggatggc acgggtagag ggcgagttat tc
//
LOCUS       DAZFAB010000450          352 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZFAB010000450 DAZFAB010000000
VERSION     DAZFAB010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.456"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>352
                     /locus_tag="ACHJWA_005287"
     CDS             <1..>352
                     /locus_tag="ACHJWA_005287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HFO4311669.1"
                     /translation="IENILDGKGDLFKKRTLWEFVRDLFPGSHIKEVKGLIYEFVTKV
                     DNKAEVFDKIKSLAKKEQQWRFSTKTDFTTNENNEVIVSRSFNLYTGATSNDNEKKQV
                     SSERLTLDNYLDDLH"
BASE COUNT          126 a           51 c           68 g          107 t
ORIGIN      
        1 tattgaaaat attcttgatg gcaaagggga tttatttaaa aaacgtacac tgtgggagtt
       61 tgttcgcgat ctttttcctg gctctcatat taaagaagtc aaaggcttaa tttacgaatt
      121 tgtcacgaaa gttgataata aagccgaggt gttcgataag attaaatctc tggcaaaaaa
      181 agaacaacaa tggcgattta gtactaagac agactttacc acaaatgaaa ataatgaagt
      241 tattgtgagt agatccttta atctctacac gggggctact tcgaatgata acgaaaaaaa
      301 acaggtttct tcagagagat taacgcttga taattatctc gatgatttac at
//
LOCUS       DAZFAB010000451          344 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZFAB010000451 DAZFAB010000000
VERSION     DAZFAB010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.457"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>344
                     /locus_tag="ACHJWA_005288"
     CDS             <1..>344
                     /locus_tag="ACHJWA_005288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311670.1"
                     /translation="HGDYHHSETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAQQN
                     LAAWMIDVIRHAMNGIQERELSLAELSWWAVCNQVVDALPEAVSRRSLGLPAEKIRSV
                     YRESDIIPGEQT"
BASE COUNT           87 a           89 c           94 g           74 t
ORIGIN      
        1 tcatggtgat tatcatcaca gcgaaactgt catacatcgt tacggtaccg gcgcgatggt
       61 gttgtgctgg cactgcgaca accagctgcg cgaccagaca tccgaatcac ttgagcaact
      121 tgctcaacag aatctggccg cctggatgat tgacgtcatc cgccacgcaa tgaatggcat
      181 acaggaacgg gaattatcgc tggctgaatt atcctggtgg gcagtctgca atcaggtggt
      241 ggacgcatta cctgaggcag tatcgcgtcg ttctctggga ttaccggcgg aaaaaatccg
      301 ctccgtatac cgtgaaagcg acatcatacc gggagaacag accg
//
LOCUS       DAZFAB010000452          341 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZFAB010000452 DAZFAB010000000
VERSION     DAZFAB010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.458"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>341
                     /locus_tag="ACHJWA_005289"
     rRNA            <1..>341
                     /locus_tag="ACHJWA_005289"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           86 a           87 c           98 g           70 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac c
//
LOCUS       DAZFAB010000453          335 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZFAB010000453 DAZFAB010000000
VERSION     DAZFAB010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.459"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>335
                     /locus_tag="ACHJWA_005290"
     CDS             <1..>335
                     /locus_tag="ACHJWA_005290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III effector protein"
                     /protein_id="HFO4311671.1"
                     /translation="NHCCLYAACLGELTGGNPLYDCAIDTHTTILSGNLAANTQVKIP
                     VYMYHRGAIAEKDILPFMEKVTNIYSRIEDIYKYSNNMKNNYKKALADLQSLLVNLHN
                     IMPGDDVKD"
BASE COUNT          112 a           50 c           61 g          112 t
ORIGIN      
        1 ttaatcattg ctgtttatat gctgcttgtt tgggcgagtt aaccggtggg aatcccttgt
       61 atgattgtgc tattgatact cataccacta tcttaagtgg taatcttgct gcaaacactc
      121 aggttaaaat tcctgtttat atgtaccata gaggagctat agctgaaaaa gacattttac
      181 ctttcatgga aaaggtaacg aatatttatt cacggataga agatatttat aaatatagca
      241 ataatatgaa gaataattac aaaaaggcac tagccgattt acaatcctta ttagttaatc
      301 tgcataacat tatgcctggt gatgatgtaa aagat
//
LOCUS       DAZFAB010000454          335 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZFAB010000454 DAZFAB010000000
VERSION     DAZFAB010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.460"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>335
                     /locus_tag="ACHJWA_005291"
     CDS             <1..>335
                     /locus_tag="ACHJWA_005291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFO4311672.1"
                     /translation="FHTRIQHGEPQVESRYLYDPLGRRTGKRVWRRERDLTGWMSLSR
                     KPEETWYGWDGDRLTTVQTQQTRIQTVYQPGSFTPLLRIETENGEQAKARHRSLAEVL
                     QEDTGVTLPA"
BASE COUNT           79 a           89 c          123 g           44 t
ORIGIN      
        1 ttccacacgc ggatacagca cggcgaacca caggtggaga gccggtacct ctatgacccg
       61 ctgggccgcc ggacgggaaa acgggtgtgg cggcgggagc gtgacctgac ggggtggatg
      121 tcgctgtcgc gtaaaccgga ggagacctgg tacgggtggg acggtgacag gctgaccact
      181 gtacagaccc aacagacaag aatccagacg gtataccagc cgggaagctt cacgccgctc
      241 ctgagaatcg aaacagagaa tggtgaacag gcgaaggcgc ggcaccgtag cctggcggag
      301 gtgttgcagg aggacacggg tgtgacgcta ccggc
//
LOCUS       DAZFAB010000455          333 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZFAB010000455 DAZFAB010000000
VERSION     DAZFAB010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.461"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>333
                     /locus_tag="ACHJWA_005292"
     CDS             <1..>333
                     /locus_tag="ACHJWA_005292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO4311673.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAK"
BASE COUNT           74 a           85 c          103 g           71 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaa
//
LOCUS       DAZFAB010000456          327 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZFAB010000456 DAZFAB010000000
VERSION     DAZFAB010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.462"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>327
                     /locus_tag="ACHJWA_005293"
     CDS             <1..>327
                     /locus_tag="ACHJWA_005293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO4311674.1"
                     /translation="STPEGEWYIPCVNISAEPEAYFRIAPEDWLRAEMQGEIVALVHS
                     HPGGLPWLSEADRRLQIKSALPWWLVCRGDIHKFRCVPHLTGRRFEHGVTDCYTLFRD
                     AYHLAG"
BASE COUNT           61 a           81 c          116 g           69 t
ORIGIN      
        1 taagcacgcc ggagggggag tggtatatcc cttgtgtgaa tatttctgca gagccggagg
       61 cgtattttcg tatcgcaccg gaagactggc tgcgggcaga gatgcagggg gagattgtgg
      121 cactggtcca cagtcatccc ggtgggctgc cctggctgag cgaggccgac cggcggctgc
      181 agataaaaag cgcactgccc tggtggctgg tctgccgggg tgacattcac aaattccgct
      241 gtgtgccaca tctgacagga cggcgctttg agcacggggt gacggactgt tacacgctgt
      301 tccgggatgc ttatcatctg gcgggga
//
LOCUS       DAZFAB010000457          322 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZFAB010000457 DAZFAB010000000
VERSION     DAZFAB010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.463"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>322
                     /locus_tag="ACHJWA_005294"
     CDS             <1..>322
                     /locus_tag="ACHJWA_005294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFO4311675.1"
                     /translation="ILTGGAAATTTAELWKMAGVISDEAGGGIRQAAENLARLAESGK
                     YTAGQLRIMGETSQRWLQTVGDDAGKVEKAFEGIAADPVKALASLNQQYNFLSVSQLR
                     HIDEL"
BASE COUNT           80 a           74 c          111 g           57 t
ORIGIN      
        1 atcctgaccg gaggggcggc tgccaccaca acggcagaat tgtggaaaat ggccggggtg
       61 atcagcgatg aagccggtgg tggtatcaga caggcggcag aaaatctggc ccgtctggca
      121 gaaagcggga aatataccgc cgggcagcta cggatcatgg gggaaacctc tcagagatgg
      181 ctgcagacgg tgggggacga tgccgggaag gtggaaaaag cctttgaagg gattgcagca
      241 gatccggtga aggcgctggc ctccctgaat cagcagtata acttcctgag cgtttcccag
      301 ttacgccata ttgatgagct tg
//
LOCUS       DAZFAB010000458          311 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZFAB010000458 DAZFAB010000000
VERSION     DAZFAB010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.464"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..>311)
                     /locus_tag="ACHJWA_005295"
     CDS             complement(<1..>311)
                     /locus_tag="ACHJWA_005295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO4311676.1"
                     /translation="GKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGG
                     AGSPAQMRRAEGINFNQNNHVVIQNDGTNGQAGPQLMKAVYDMARKGAQDELRLQLRD
                     G"
BASE COUNT           60 a          118 c           65 g           68 t
ORIGIN      
        1 cgccatcacg caactgcagc cggagctcat cctgcgcccc cttgcgggcc atgtcataca
       61 ccgccttcat cagctgcggc cccgcctgtc cgttggtgcc gtcgttctga atcaccacgt
      121 gattgttctg attaaaatta ataccttccg cccgccgcat ctgcgccgga cttccggcac
      181 cacccacata accaccttcc gcatagccgc gcatcagacg gtaaagattc cccacaccta
      241 tccggctggt tgcctctttc gtgaaaacaa actccccgcg gtgaactatc cccgcaggct
      301 catatttgcc g
//
LOCUS       DAZFAB010000459          306 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZFAB010000459 DAZFAB010000000
VERSION     DAZFAB010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.465"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>306
                     /gene="espL"
                     /locus_tag="ACHJWA_005296"
     CDS             <1..>306
                     /gene="espL"
                     /locus_tag="ACHJWA_005296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE stator protein
                     EspL"
                     /protein_id="HFO4311677.1"
                     /translation="VNQSIKWVKDIQAIELVLMQDIMNKIHLSLTNALHSLDTSSRIN
                     WDDLLNEVVRETLSNNNIVGAIKITKNPDIKLDPGEANNIQLINDANTPHNKIIIEN"
BASE COUNT          119 a           48 c           51 g           88 t
ORIGIN      
        1 ggttaaccaa tcgataaaat gggttaaaga tatccaggct atcgagttgg tcttaatgca
       61 ggacataatg aataagattc atttgtcact gactaatgca ttacattctt tagatacatc
      121 atcaagaatt aattgggatg atttacttaa tgaagttgta agggaaactc tatctaataa
      181 caatattgtc ggagctatta agatcactaa aaacccagac attaaacttg atcctggcga
      241 ggccaacaat atacagctta ttaatgatgc aaacacaccg cataataaaa ttataataga
      301 aaatga
//
LOCUS       DAZFAB010000460          305 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZFAB010000460 DAZFAB010000000
VERSION     DAZFAB010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.466"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>305
                     /locus_tag="ACHJWA_005297"
     CDS             <1..>305
                     /locus_tag="ACHJWA_005297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4311678.1"
                     /translation="AWCLWDMLTHPRYGMGKRLVAADVDKWALYAIGQYCDQTVPDGF
                     GGTEPRMTFNAYLSQQRKVWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDVVWPYTS"
BASE COUNT           55 a           78 c          107 g           65 t
ORIGIN      
        1 gcctggtgcc tgtgggacat gctgactcac ccgcgctacg gcatgggaaa acgtctggtg
       61 gcggcggatg ttgacaagtg ggcgctgtat gccatcgggc agtactgcga ccagacggtc
      121 ccggatggtt tcgggggcac agagccgcgg atgaccttta atgcgtacct gtcacaacag
      181 cgtaaggtat gggatgtgct cagtgatttc tgctcggcga tgcgctgtat gccggtatgg
      241 aacggccaga cgctgacgtt cgttcaggac cgtccgtcgg atgtggtgtg gccgtacacc
      301 agcag
//
LOCUS       DAZFAB010000461          304 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZFAB010000461 DAZFAB010000000
VERSION     DAZFAB010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.467"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..282
                     /locus_tag="ACHJWA_005298"
     CDS             <1..282
                     /locus_tag="ACHJWA_005298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311679.1"
                     /translation="WEQKFARHVQHVRAQVKPVSKGVSHAAPGGTASRAVQEIRAARE
                     QWERENGFISNGNGLEAVGTYGGGVFEPLDPEERGCAVEALDCSDWRDD"
BASE COUNT           81 a           72 c          102 g           49 t
ORIGIN      
        1 tgggagcaga aatttgcccg tcacgtgcag cacgtcaggg cacaggtaaa accagtcagc
       61 aagggggtaa gccatgcagc accaggtggc accgcatcac gggcagttca ggaaattcgg
      121 gcagcacgtg aacagtggga acgtgaaaac ggatttatca gcaacggaaa cggcctggaa
      181 gctgtgggaa cttatggggg aggtgtattc gaaccgctgg acccagaaga acggggctgc
      241 gccgtcgaag ctctggattg ctcagattgg cgcgatgact gaacagcaaa tccggcaggt
      301 ctgc
//
LOCUS       DAZFAB010000462          303 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZFAB010000462 DAZFAB010000000
VERSION     DAZFAB010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.468"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..>303)
                     /locus_tag="ACHJWA_005299"
     CDS             complement(<1..>303)
                     /locus_tag="ACHJWA_005299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311680.1"
                     /translation="IIRSYIHPDEDNKFDNFMRQNRFSKVNFDTQQMIDFINRFNMNK
                     WLIDRNNNFFIQLIDQALRSTNDTIKENAWHLYKEWIRSDDVSPLFIEIEDNLRTFN"
BASE COUNT          101 a           49 c           44 g          109 t
ORIGIN      
        1 gttaaaggtt cttaaattat cttcaatttc tataaataaa ggtgaaacat catcactacg
       61 aatccactct ttataaagat gccaggcatt ttctttgatc gtatcattcg ttgatcgtag
      121 agcctgatcg ataagttgga taaaaaaatt gttatttcga tcaatcagcc atttattcat
      181 attaaatctg ttaataaaat cgatcatctg ttgggtatcg aaattcacct tactgaatcg
      241 attttgacgc ataaaattat cgaacttgtt atcttcatca ggatggatat aactacgtat
      301 aat
//
LOCUS       DAZFAB010000463          294 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZFAB010000463 DAZFAB010000000
VERSION     DAZFAB010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.469"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>294
                     /locus_tag="ACHJWA_005300"
     rRNA            <1..>294
                     /locus_tag="ACHJWA_005300"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           74 a           67 c          101 g           52 t
ORIGIN      
        1 agcggtgaaa tgcgtagaga tctggaggaa taccggtggc gaaggcggcc ccctggacga
       61 agactgacgc tcaggtgcga aagcgtgggg agcaaacagg attagatacc ctggtagtcc
      121 acgccgtaaa cgatgtcgac ttggaggttg tgcccttgag gcgtggcttc cggagctaac
      181 gcgttaagtc gaccgcctgg ggagtacggc cgcaaggtta aaactcaaat gaattgacgg
      241 gggcccgcac aagcggtgga gcatgtggtt taattcgatg caacgcgaag aacc
//
LOCUS       DAZFAB010000464          285 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZFAB010000464 DAZFAB010000000
VERSION     DAZFAB010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.470"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
BASE COUNT          122 a           63 c           30 g           70 t
ORIGIN      
        1 acctccgttt atctacagac tgccatcctg aatttaccgt ccttaataag acaacaataa
       61 aaaactaatt atataaaaac aataaaaata atatacaaaa aaactaaaca acaaagaaaa
      121 gcaaatcata tctggcattt taattgaaag agcaattacc gaacaaaaga cgctgactat
      181 atactcaaaa ccaaacaaca attctgccaa tcaggtatca tggcaacaca cggaattacc
      241 atgtttttgc cttctctgcc catacaatac gggcatatac ttcat
//
LOCUS       DAZFAB010000465          284 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZFAB010000465 DAZFAB010000000
VERSION     DAZFAB010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.471"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..219)
                     /locus_tag="ACHJWA_005301"
     CDS             complement(<1..219)
                     /locus_tag="ACHJWA_005301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HFO4311681.1"
                     /translation="MSEKTEKPTPKKLRDLKKKGDVTKSEEVMAAVQSLILFSFFSLY
                     GMSFFVDIVGLVNTTIDSLNRPFLYAIRE"
     gene            complement(212..>284)
                     /locus_tag="ACHJWA_005302"
     CDS             complement(212..>284)
                     /locus_tag="ACHJWA_005302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311682.1"
                     /translation="PDFITANIHSDIIDRSLPSMINE"
BASE COUNT           90 a           54 c           41 g           99 t
ORIGIN      
        1 ttctcgaatg gcatacaaaa acggtctatt tagcgagtct attgtcgtat taactaatcc
       61 aactatatca acaaaaaaac tcatgccata taaagaaaaa aatgaaaata agattaatga
      121 ctgcactgca gccattacct cttcactttt tgttacatcg cccttctttt ttagatccct
      181 tagttttttg ggtgtgggct tttctgtttt ttcactcatt aatcatgctc ggtaacgatc
      241 tatcaattat gtcagaatga atatttgccg taataaaatc agga
//
LOCUS       DAZFAB010000466          283 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZFAB010000466 DAZFAB010000000
VERSION     DAZFAB010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.472"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>283
                     /locus_tag="ACHJWA_005303"
     rRNA            <1..>283
                     /locus_tag="ACHJWA_005303"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           72 a           62 c           96 g           53 t
ORIGIN      
        1 cacggcgggt gctaacgtcc gtcgtgaaga gggaaacaac ccagaccgcc agctaaggtc
       61 ccaaagtcat ggttaagtgg gaaacgatgt gggaaggccc agacagccag gatgttggct
      121 tagaagcagc catcatttaa agaaagcgta atagctcact ggtcgagtcg gcctgcgcgg
      181 aagatgtaac ggggctaaac catgcaccga agctgcggca gcgacactgt gtgttgttgg
      241 gtaggggagc gttctgtaag cctgtgaagg tgtgctgtga ggc
//
LOCUS       DAZFAB010000467          278 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZFAB010000467 DAZFAB010000000
VERSION     DAZFAB010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.473"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            24..100
                     /locus_tag="ACHJWA_005304"
     tRNA            24..100
                     /locus_tag="ACHJWA_005304"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:58..60,aa:Ile,seq:gat)
     gene            143..218
                     /locus_tag="ACHJWA_005305"
     tRNA            143..218
                     /locus_tag="ACHJWA_005305"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:176..178,aa:Ala,seq:tgc)
BASE COUNT           75 a           62 c           73 g           68 t
ORIGIN      
        1 aaaatgagca gtaaaacctc tacaggcttg tagctcaggt ggttagagcg cacccctgat
       61 aagggtgagg tcggtggttc aagtccactc aggcctacca aatttgcacg gcaaatttga
      121 agaggtttta actacatgtt atggggctat agctcagctg ggagagcgcc tgctttgcac
      181 gcaggaggtc tgcggttcga tcccgcatag ctccaccatc tctgtagtga ttaaataaaa
      241 aatacttcag agtgtacctg caaaggttca ctgcgaag
//
LOCUS       DAZFAB010000468          271 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZFAB010000468 DAZFAB010000000
VERSION     DAZFAB010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.474"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
BASE COUNT           76 a           48 c           66 g           81 t
ORIGIN      
        1 atcacgtgag agctgcacat tattggaggg gcaggggaaa acaccatttt gggaacaatg
       61 aatcaggtac tcttaccact gaagcaagat catccgggca ccaggtggtg gtgcagtgct
      121 ttggaaatgg tcccctcggg ggagttgtca ttattttcta tgctatttgc ttatgatatt
      181 catcaggtga atttaataaa ttcctaattt gaaatatcca ctattaagcc agtgtttaac
      241 gacgttaaga tggcaatgtg cagatcatgt t
//
LOCUS       DAZFAB010000469          270 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZFAB010000469 DAZFAB010000000
VERSION     DAZFAB010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.475"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>270
                     /locus_tag="ACHJWA_005306"
     CDS             <1..>270
                     /locus_tag="ACHJWA_005306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311683.1"
                     /translation="LSHMWYSEISEYLISRGKSFATSAWVKDALKHTYLGYETKDLVD
                     VVTGEITTIQSLRHTSDLGTGEMYVFLCKVEAWAMNIGCHLTIPQ"
BASE COUNT           74 a           67 c           64 g           65 t
ORIGIN      
        1 actcagccac atgtggtaca gcgaaatcag tgaatacctc atcagcaggg ggaaatcgtt
       61 cgctacttca gcatgggtaa aagatgctct caaacacaca tacctcggtt atgaaaccaa
      121 ggacctggtt gatgtcgtaa ccggcgaaat cactactatc cagtcgttac gccatacctc
      181 cgatcttggt accggagaga tgtatgtctt cctgtgtaag gttgaagcct gggcgatgaa
      241 tattggctgc cacctgacta ttccacagag
//
LOCUS       DAZFAB010000470          269 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZFAB010000470 DAZFAB010000000
VERSION     DAZFAB010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..269
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.476"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            18..>269
                     /locus_tag="ACHJWA_005307"
     CDS             18..>269
                     /locus_tag="ACHJWA_005307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311684.1"
                     /translation="MITHIPRSSFSANINNTAQTNEHQTLSELFYKELEDKFSGKELA
                     TPLLKSFSENCRHNGRHIFSNKDFVIKFSISVLQADKKEI"
BASE COUNT           95 a           49 c           43 g           82 t
ORIGIN      
        1 aacaatgaga tatttaaatg attactcata ttcctcgtag ttctttctct gcaaatatta
       61 ataatacagc ccagacaaat gaacaccaaa ccctgagtga attgttttat aaagaactcg
      121 aggataaatt ttctggcaag gagctggcga cacctctatt aaaaagcttc tcagagaact
      181 gtagacacaa tggtcgccat atctttagta acaaggattt tgtcattaag ttttccatat
      241 ctgtcttaca agctgataag aaagaaatt
//
LOCUS       DAZFAB010000471          266 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZFAB010000471 DAZFAB010000000
VERSION     DAZFAB010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.477"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>266
                     /locus_tag="ACHJWA_005308"
     CDS             <1..>266
                     /locus_tag="ACHJWA_005308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO4311685.1"
                     /translation="KVSDMQKNVTPGRRKGCPNYPPEFKQQLVAASCEPGISISKLAL
                     ENGINANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPE"
BASE COUNT           75 a           74 c           57 g           60 t
ORIGIN      
        1 ccaaagtatc agatatgcag aaaaatgtga ctcccggcag gcgaaaaggc tgccctaatt
       61 atcctcccga atttaaacag cagctcgttg ctgcctcctg tgaacccggg atatccatct
      121 caaaacttgc tcttgaaaat ggcattaacg ccaatctgtt gttcaaatgg cgacaacaat
      181 ggcgcgaggg aaagctgcta ttaccttctt cagagagccc ccagctactt cctgtgactc
      241 tcgatgcagc tgccgaacag ccagaa
//
LOCUS       DAZFAB010000472          259 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZFAB010000472 DAZFAB010000000
VERSION     DAZFAB010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..259
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.478"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>259
                     /locus_tag="ACHJWA_005309"
     CDS             <1..>259
                     /locus_tag="ACHJWA_005309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFO4311686.1"
                     /translation="IRIPYATDPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYRHD
                     EYGRLTEKTDRIPAGVIRTDDERTHHYHYDSQHRLVFYTRIQ"
BASE COUNT           60 a           88 c           80 g           31 t
ORIGIN      
        1 tatccgcatc ccgtatgcga ccgacccggc gggaaaccgg ctgccggacc cggagctaca
       61 cccggacagc acgctcacgg cgtggccgga taaccgcatc gcggaggatg cgcactatgt
      121 ctaccgacac gatgaatacg gcaggctgac ggagaagacg gaccgcatcc cggcgggtgt
      181 gatacggacg gacgacgagc ggacccacca ctaccactac gacagccagc accgcctggt
      241 gttctacacg cggatacag
//
LOCUS       DAZFAB010000473          256 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZFAB010000473 DAZFAB010000000
VERSION     DAZFAB010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.479"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(46..>256)
                     /locus_tag="ACHJWA_005310"
     CDS             complement(46..>256)
                     /locus_tag="ACHJWA_005310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4311687.1"
                     /translation="LKTYRVTLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKV
                     TCAGWSARVGMLRVEFSAEFKQVVN"
BASE COUNT           54 a           88 c           56 g           58 t
ORIGIN      
        1 ctgatttaac cgactcgttc agactttctt cgtgaatatc ctgcatcagt tcaccacctg
       61 cttaaactcc gcgctgaact caacgcgcaa catcccgacc cgcgcagacc acccggcaca
      121 ggtcaccttt atctgccggt atgcataggg tggcttccac aaaaatgcct tccagcctcc
      181 gtgctctgcc aggaacgctt ccagatgccg ggcctcctcc cgggtcacgg aaagcgtcac
      241 acggtatgtt ttcagg
//
LOCUS       DAZFAB010000474          253 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZFAB010000474 DAZFAB010000000
VERSION     DAZFAB010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.denovo.480"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(47..151)
                     /locus_tag="ACHJWA_005311"
     CDS             complement(47..151)
                     /locus_tag="ACHJWA_005311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4311688.1"
                     /translation="MSDDISLAMEGALAVIAVVGVYCLVVFLMDRLGN"
BASE COUNT           85 a           72 c           38 g           58 t
ORIGIN      
        1 gcaaaccgtt tttacccgat atgggaattc ccatatcgta atgaattcag ttccctagtc
       61 gatccatcaa aaacacaacc aggcagtaaa cgcccacaac agcaataaca gccagcgcac
      121 cttccattgc cagtgaaata tcatccgaca tattccctcc tttggtgtga atcccggcga
      181 acgtttttac ccccaccgac aaataacata tactaaaaaa tcaatagcta tagcaacgcc
      241 tgtaattgca aag
//
LOCUS       DAZFAB010000475         4538 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.guided.202, whole genome shotgun sequence.
ACCESSION   DAZFAB010000475 DAZFAB010000000
VERSION     DAZFAB010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4538
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.guided.202"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            26..1159
                     /locus_tag="ACHJWA_005312"
     CDS             26..1159
                     /locus_tag="ACHJWA_005312"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO4311689.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIVNGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            1247..1780
                     /gene="blc"
                     /locus_tag="ACHJWA_005313"
     CDS             1247..1780
                     /gene="blc"
                     /locus_tag="ACHJWA_005313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO4311690.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(1777..2094)
                     /gene="sugE"
                     /locus_tag="ACHJWA_005314"
     CDS             complement(1777..2094)
                     /gene="sugE"
                     /locus_tag="ACHJWA_005314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO4311691.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2270..2416)
                     /gene="ecnB"
                     /locus_tag="ACHJWA_005315"
     CDS             complement(2270..2416)
                     /gene="ecnB"
                     /locus_tag="ACHJWA_005315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFO4311692.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(2527..2652)
                     /gene="ecnA"
                     /locus_tag="ACHJWA_005316"
     CDS             complement(2527..2652)
                     /gene="ecnA"
                     /locus_tag="ACHJWA_005316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFO4311693.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(2704..3270)
                     /gene="efp"
                     /locus_tag="ACHJWA_005317"
     CDS             complement(2704..3270)
                     /gene="efp"
                     /locus_tag="ACHJWA_005317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFO4311694.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            3312..4340
                     /gene="epmB"
                     /locus_tag="ACHJWA_005318"
     CDS             3312..4340
                     /gene="epmB"
                     /locus_tag="ACHJWA_005318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFO4311695.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RNAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRAGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         1167 a         1149 c         1119 g         1103 t
ORIGIN      
        1 ccgttttgta tggaaaccag accctatgtt caaaacgacg ctctgcgcct tattaattac
       61 cgcctcttgc tccacatttg ctgcccctca acaaatcaac gatattgtgc atcgcacaat
      121 taccccgctt atagagcaac aaaagatccc cggtatggcg gtggcggtaa tttatcaggg
      181 taaaccttat tactttacct gggggtatgc ggacatcgcc aaaaagcagc ccgtcacaca
      241 gcaaacgttg tttgagttag gttcggtcag caaaacattt acgggcgtgc ttggtggcga
      301 cgctattgct cgaggggaaa tcaagttaag cgatcccgca acaaaatact ggcctgaact
      361 taccgctaaa cagtggaatg ggatcacact attacatctc gcaacctaca ctgctggcgg
      421 cctgccattg caggtgccgg atgaagtgaa atcctcaagc gacttgctgc gcttctatca
      481 aaactggcag cctgcatggg ctccaggaac acaacgtctg tatgccaact ccagtatcgg
      541 tttgttcggc gcactggctg tgaagccgtc tggtttgagt tttgagcagg cgatgcaaac
      601 tcgtgtcttc cagccactca aactcaacca tacgtggatt aatgtaccgc ccgcagaaga
      661 aaagaattac gcctggggat atcgcgaagg caaggcagtt catgtttcgc caggggcgtt
      721 agatgctgaa acttatggtg tgaagtcgac cattgaagat atggcctgct gggtacgaag
      781 caatatgaat ccccgtgata tcaacgacaa aacacttcag caagggatac aactggcaca
      841 atctcgctac tggcaaaccg gcgatatgta tcagggtctg ggctgggaaa tgctggactg
      901 gccggtaaat cctgacatca tcgttaacgg cagtgacaat aaaattgcac tggcagcacg
      961 ccccgtaaaa gcgattacgc ctccaactcc tgcagtacga gcatcatggg tgcataaaac
     1021 gggggcgacc ggcggatttg gtagctatgt cgcgtttatt ccagaaaaag agctgggtat
     1081 cgtgatgctg gctaacaaaa actatcccaa tccagcgaga gtcgccgccg cctggcagat
     1141 tctcaacgct ctacagtaaa atttcatcgg gtccgaattt tcggaccttt tctgcgcttt
     1201 tccttgctgt catctacact tagaaaaaac cagtaaggaa acatttatgc gcctgctccc
     1261 tctcgttgcc gcagcgacag ctgcatttct ggtcgttgcc tgcagttctc ctacgccgcc
     1321 gcgtggcgtg accgtagtga ataatttcga cgctaaacgc tatctcggta cctggtatga
     1381 gattgcccgt tttgatcacc gctttgaacg tggactggaa aaagtcaccg caacatacag
     1441 cctgcgtgat gacggcggcc tgaatgtcat taataaaggc tataaccctg acagaggaat
     1501 gtggcagcag agtgaaggga aagcgtactt taccggcgca ccaactcgcg ctgcgctgaa
     1561 agtgtcattc tttggtcctt tctatggcgg ttataacgtt attgcactcg atcgggaata
     1621 ccgccatgcg ctggtttgcg gaccggaccg cgactacctg tggatactct cccgcacgcc
     1681 aaccatttct gacgaagtga aacaggagat gctggcagtc gcgacccggg aagggtttga
     1741 tgtcagtaag tttatttggg tacagcagcc cggtagttag tgagtgctga gtttcagacc
     1801 aataatcccc aatacgatta acgccagact cgccagacgc atcgggttag cggactcacc
     1861 gagcagcaca atgccggtga tggccgcgcc gactgcgcca atacccgtcc acacggcata
     1921 agccgtccct actggtaacg atttcatcgc ccaggcaagt agcgccatac tgacaatcat
     1981 cgccgtcacg gtaataacac tcggcgtcaa acgactaaag ccgtgggtat atttcaggcc
     2041 tacggcccat accacttcca gcagaccagc aataactaag ataatccagg acatatcagg
     2101 ctccggagaa ttggggccgt ccccggtgaa aagacgcgtt tgcaggtcgt cctgcaaagc
     2161 tgatggttga acggatattt tgccaggtgg cgggggaatt tcaactatta cctttgtgac
     2221 tatcgcagaa aaaacaaacg gcacgacaca gctgtcgtac cgttattgct tattgctgcg
     2281 ctttcgttgc tgcaccagaa atcgcgttac cgccatcaga aatgtcttca ccaacgccac
     2341 gcgtggtgtt gcaggcagtt aatactgttg aaagcaccag aacagaaaag atcgctgcaa
     2401 ttgtcttctt caccataacg tcttcctttt aggtaatgtt atttatgttt gcctatagca
     2461 aattaataat agacaacatc gccaaaaatg atggaaatct gatgattttt cggaagagaa
     2521 gaaaaattag ctggcagcgc gggagatgga gttgccgaga tgtttgatgt cttcgccgaa
     2581 accgcgagcg gtgttacagc ccgtgagcag cgtgctggca agcaaaacaa gaacgataag
     2641 gcgtttcatc atctctgacc tgaataagtg atggtgcagc ctgcaggccg caccacaacc
     2701 gcattacttc acgcgagaga cgtattcacc agagcgggta tccactttga tgacttcgcc
     2761 gatttgtacg aacagcggaa ctttaaccac agcgccagta gacagggtag ccggtttgcc
     2821 gccagtacct gcggtatcac ctttcaggcc cggatcggta tcaacgattt ccagttcaac
     2881 gaagttcggc ggagtaacgg agatcggctg accattccac agagttacga tacactctgc
     2941 ctgatccagc agccatttag cgttgtcgcc aattgctttt gcgtcagcag acagctgctc
     3001 gaaagtttcg ttgttcatga agtgccagaa ctcaccgtcg ttgtacaggt aagtcaggtt
     3061 catatcgaca acatcagcgc cttcagcgga atcagtagat ttgaaggttt tttctacgcg
     3121 agtaccggtc agcagacgac gcagtttaac gcgagcaaat gcctggcctt tacccggttt
     3181 tacgaattca ctcgcttcaa ccgcgtaagg ttcgccgtct aacatgattt taagaccagc
     3241 acgaaaatcg ttgctatagt acgttgccat aaggccctct gaaatttgtt aattggtagc
     3301 taagccacaa aatggcgcat attgtaaccc taaatacccc atccagagaa gattggttaa
     3361 cgcaacttgc cgatgttgtg accgatcctg atgaacttct gcgtcttttg aatatagacg
     3421 cggacgaaaa actgttggcc ggacgcaacg ccaaaaagct gtttgccctg cgtgtgcccc
     3481 gctcatttat cgatcgcatg gagaaaggca atccggacga tccgcttttg cgtcaggtac
     3541 ttacctcgca agatgagttt gtcgtcgcgc ccggattctc caccgacccg ctggaagaac
     3601 agcacagcgt agtgcctggt ttgttgcata aataccacaa ccgggcgctt ttgctggtca
     3661 aaggcggctg cgcggtaaat tgccgctatt gcttccgtcg ccacttcccc tatgccgaaa
     3721 atcagggcaa caagcgtaac tggcaaactg cacttgagta tgttgctgcg catccggaac
     3781 tggacgagat gattttctcc ggcggcgatc cgctgatggc gaaagatcac gagctggact
     3841 ggttgctcac acaactggaa gccatcccgc atatcaaacg tctgcggatt cacagtcgtc
     3901 tgccgattgt gatcccggca cgcatcacag atgcgctggt tgaacgcttt tcacattcta
     3961 cgctgcaaat cttgctggtg aatcacatca accatgccaa tgagatagat gaaacattcc
     4021 gtcaggcgat ggctaagttg cgccgggcgg gcgttacttt gctgaaccag agcgttctgt
     4081 tacgtggtgt gaacgataac gcacaaacgc tggcaaacct gagtaatgcg ttgttcgatg
     4141 caggcgtgat gccgtattac ctgcatgtgc tcgataaagt acagggcgcg gcgcatttta
     4201 tggtgagtga tgacgaagca cggcagatta tgcgtgagtt gctgacactg gtgtcgggat
     4261 atctggtgcc gaaactggcg cgagaaattg gcggcgaacc cagcaaaacg ccgctggatc
     4321 tccagctacg ccagcagtaa ctgattaaac agggaagttt aatctttgcc cgttacataa
     4381 cctttaatta ttcatataca ttgctgttgt tactctttat ataaccaaca ccaataatta
     4441 acctttcttt tctgtttata tagagtgagt acggctattt atgttatttt aacaactatt
     4501 actaccaagc ccatttatct ggcttataaa ttaaaatt
//
LOCUS       DAZFAB010000476         2748 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.guided.237, whole genome shotgun sequence.
ACCESSION   DAZFAB010000476 DAZFAB010000000
VERSION     DAZFAB010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.guided.237"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..110
                     /locus_tag="ACHJWA_005319"
     CDS             <1..110
                     /locus_tag="ACHJWA_005319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFO4311696.1"
                     /translation="GFWLDDAQVVDYRVVKMPVTKGGRLELTITEMGNE"
     gene            107..247
                     /locus_tag="ACHJWA_005320"
     CDS             107..247
                     /locus_tag="ACHJWA_005320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFO4311697.1"
                     /translation="MMFEFNMAELLRHRWGRLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            333..767
                     /locus_tag="ACHJWA_005321"
     CDS             333..767
                     /locus_tag="ACHJWA_005321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO4311698.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLIPEKVKSRPQ
                     CCDDDAMVICGCIARLYRNNRDLHDLLVDYYVLGETFMALARKHGCSDTCIGKRLHKA
                     EGIVEGMLMMLGVRLEMDRYVERELPGGRTSVFYQRKNSLRS"
     gene            1274..2221
                     /gene="stxA1a"
                     /locus_tag="ACHJWA_005322"
     CDS             1274..2221
                     /gene="stxA1a"
                     /locus_tag="ACHJWA_005322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit A"
                     /protein_id="HFO4311699.1"
                     /translation="MKIIIFRVLTFFFVIFSVNVVAKEFTLDFSTAKTYVDSLNVIRS
                     AIGTPLQTISSGGTSLLMIDSGTGDNLFAVDVRGIDPEEGRFNNLRLIVERNNLYVTG
                     FVNRTNNVFYRFADFSHVTFPGTTAVTLSGDSSYTTLQRVAGISRTGMQINRHSLTTS
                     YLDLMSHSGTSLTQSVARAMLRFVTVTAEALRFRQIQRGFRTTLDDLSGRSYVMTAED
                     VDLTLNWGRLSSVLPDYHGQDSVRVGRISFGSINAILGSVALILNCHHHASRVARMAS
                     DEFPSMCPADGRVRGITHNKILWDSSTLGAILMRRTISS"
     gene            2231..2500
                     /gene="stxB1a"
                     /locus_tag="ACHJWA_005323"
     CDS             2231..2500
                     /gene="stxB1a"
                     /locus_tag="ACHJWA_005323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit B"
                     /protein_id="HFO4311700.1"
                     /translation="MKKTLLIAASLSFFSASALATPDCVTGKVEYTKYNDDDTFTVKV
                     GDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR"
BASE COUNT          720 a          492 c          706 g          830 t
ORIGIN      
        1 caggtttctg gctggatgat gctcaggtcg ttgattaccg cgttgtgaag atgcctgtta
       61 ccaaaggtgg gaggctggaa ctgaccatca ccgaaatggg gaatgaatga tgtttgagtt
      121 taatatggca gaacttcttc gccaccgctg ggggcgtctg cgcttatatc gtttccccgg
      181 ttctgttttg accgattacc gaatactgaa gaattacgcc aaaaccctga caggagcagg
      241 agtatgaagt cagagataac aatcaactaa tactgttttg ttgatttttg cttgtaattg
      301 gcgttctggt ctgatttttg tggagtaagt tgatgcgtga tattcagatg gttcttgagc
      361 gttggggagc gtgggcggct aataatcatg aagatgtgac ctggtcgtcc attgccgccg
      421 gttttaaggg actgatcccc gaaaaagtaa aatcacgccc gcagtgctgt gacgatgatg
      481 cgatggtgat atgcgggtgt atagcccgcc tttaccggaa caatcgcgat ctgcatgact
      541 tgctggttga ttattacgtg ttgggggaga cgttcatggc gctggcacgg aaacatgggt
      601 gctctgacac ctgtataggt aaacgccttc acaaagcgga ggggattgtt gaaggcatgc
      661 tgatgatgct gggagtgagg cttgagatgg atcggtatgt tgagcgtgaa ttgccgggag
      721 ggagaacctc tgtattttat cagcgaaaaa atagtttacg atcgtaaaaa tctgcatatc
      781 atgataagag tggttacatt gccacgctgc ttaacccgcc gatgcgcggg tttttttgta
      841 cccagaatcc tgtgagctat acggaaagta cacagaaagg aaggtgcgac cacaattaat
      901 aacaaaatct taaaaattgc acatggcact attagttttc taaatattgt gtattttttg
      961 tattgcagga tgaccctgta acgaagtttg cgtaacagca ttttgctcta cgagtttgcc
     1021 agcctccccc agtggctggc ttttttatgt ccgtaacatc ctgtgtatca ataaatgttg
     1081 ttatctacgt acgtcaagta gtcgcatgag atctgaccag atatgttaag gttgcagctc
     1141 tctttgaata tgattatcat tttcattacg ttattgttac gtttatccgg tgcgccgtaa
     1201 aacgccgtcc ttcagggcgt ggaggatgtc aagaatatag ttatcgtatg gtgctcaagg
     1261 agtattgtgt aatatgaaaa taattatttt tagagtgcta acttttttct ttgttatctt
     1321 ttcagttaat gtggtggcga aggaatttac cttagacttc tcgactgcaa agacgtatgt
     1381 agattcgctg aatgtcattc gctctgcaat aggtactcca ttacagacta tttcatcagg
     1441 aggtacgtct ttactgatga ttgatagtgg cacaggggat aatttgtttg cagttgatgt
     1501 cagagggata gatccagagg aagggcggtt taataatcta cggcttattg ttgaacgaaa
     1561 taatttatat gtgacaggat ttgttaacag gacaaataat gttttttatc gctttgctga
     1621 tttttcacat gttacctttc caggtacaac agcggttaca ttgtctggtg acagtagcta
     1681 taccacgtta cagcgtgttg cagggatcag tcgtacgggg atgcagataa atcgccattc
     1741 gttgactact tcttatctgg atttaatgtc gcatagtgga acctcactga cgcagtctgt
     1801 ggcaagagcg atgttacggt ttgttactgt gacagctgaa gctttacgtt ttcggcaaat
     1861 acagagggga tttcgtacaa cactggatga tctcagtggg cgttcttatg taatgactgc
     1921 tgaagatgtt gatcttacat tgaactgggg aaggttgagt agcgtcctgc ctgactatca
     1981 tggacaagac tctgttcgtg taggaagaat ttcttttgga agcattaatg caattctggg
     2041 aagcgtggca ttaatactga attgtcatca tcatgcatcg cgagttgcca gaatggcatc
     2101 tgatgagttt ccttctatgt gtccggcaga tggaagagtc cgtgggatta cgcacaataa
     2161 aatattgtgg gattcatcca ctctgggggc aattctgatg cgcagaacta ttagcagttg
     2221 agggggtaaa atgaaaaaaa cattattaat agctgcatcg ctttcatttt tttcagcaag
     2281 tgcgctggcg acgcctgatt gtgtaactgg aaaggtggag tatacaaaat ataatgatga
     2341 cgataccttt acagttaaag tgggtgataa agaattattt accaacagat ggaatcttca
     2401 gtctcttctt ctcagtgcgc aaattacggg gatgactgta accattaaaa ctaatgcctg
     2461 tcataatgga gggggattca gcgaagttat ttttcgttga ctcagaatag ctcagtgaaa
     2521 atagcaggcg gagattcata aatgttaaat acatctcaat tcagtcagtt gttgccggtc
     2581 tgataataga tgtgttagaa aatttctgca tggtgaatcc ccctgtgcgg aggggcgact
     2641 ggtgaacggt atgatctctt tgatgatcgt aagcgagaat acgcgggttt ggtggcacca
     2701 ggccgaactc accgggaggc acccggcatc atgctgtata cagagatt
//
LOCUS       DAZFAB010000477         1972 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.guided.264, whole genome shotgun sequence.
ACCESSION   DAZFAB010000477 DAZFAB010000000
VERSION     DAZFAB010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.guided.264"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..577)
                     /locus_tag="ACHJWA_005324"
     CDS             complement(<1..577)
                     /locus_tag="ACHJWA_005324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HFO4311701.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHL"
     gene            838..1296
                     /gene="gpt"
                     /locus_tag="ACHJWA_005325"
     CDS             838..1296
                     /gene="gpt"
                     /locus_tag="ACHJWA_005325"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO4311702.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            1388..>1972
                     /locus_tag="ACHJWA_005326"
     CDS             1388..>1972
                     /locus_tag="ACHJWA_005326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO4311703.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFP"
BASE COUNT          472 a          517 c          506 g          477 t
ORIGIN      
        1 ctaaatgcag gttggaggtg aagtcgatac cccccgcgca acccatgtag atttcacctt
       61 cttcttcgga gtcggtgtta atcagaatat cagcctgcaa ccagttgctc tgtaagccga
      121 acgcaccgtc cataccggct tcttcggtca tggtcagcag cacttccagc gggccgtgaa
      181 ccacgttttc gtcagccaga accgccagtg cagaggccat accaatgccg ttatccgcac
      241 ccagcgtggt gccgcgcgct ttaacccatt cgccatcaat atatggctgg ataggatctt
      301 tcgtgaagtc atgcacggtg tcgttatttt tctgcggcac catatcgagg tgggcctgca
      361 agacgaccgg tttacgattt tccatacctg cggtagcagg tttacgaatc aggatattac
      421 ctacctgatc gcgttcgaca tggaaacctt tctcttttgc ccaaccaaca atgtattcag
      481 cgagttgctc ttcatgatag gacgggtgag gaatagaaca gattttggca aaaatatccc
      541 acagcggctg tggagataat tgagacagtt cagacacgtt aagtctcctt gtcgatcacc
      601 cgcaaaacag tattgcaggt cacagggtta gcagaaaatg ttgtcaacac aagacaggct
      661 tgcgagatat gtttgagaat accactttat cccgcgtcag ggagaggcag tgcgtaaaaa
      721 gacgcggact catgtgaaat actggttttt agtgcgccag atctctataa tctcgcgcaa
      781 cctattttcc cctcgaacac tttttaagcc gtagataaac aggctgggac acttcacatg
      841 agcgaaaaat acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc
      901 cgactgatgc cttctgaaca atggaaaggc attattgccg taagccgtgg cggtctggta
      961 ccgggtgcgt tactggcgcg tgaactgggt attcgtcatg tcgataccgt ttgtatttcc
     1021 agctacgatc acgacaacca gcgcgagctt aaagtgctga aacgcgcaga aggcgatggc
     1081 gaaggcttca tcgttattga tgacctggtg gataccggtg gtactgcggt tgcgattcgt
     1141 gaaatgtatc caaaagcgca ctttgtcacc atctttgcaa aaccggctgg tcgtccgctg
     1201 gttgatgatt atgttgttga tatcccgcaa gatacctgga tcgaacagcc gtgggatatg
     1261 ggcgtcgtat tcgtcccgcc aatctccggt cgctaatctt ttcaacgcct ggcactgccg
     1321 ggcgttgttc tttttaactt caggcgagtt acaatagttt ccagtaagta ttctggaggc
     1381 tgcatccatg acacaggcaa acctgagcga aaccctgttc aaaccccgct ttaaacatcc
     1441 tgaaacctcg acgctagtcc gccgctttaa tcacggcgca caaccgcctg tgcagtcggc
     1501 ccttgatggt aaaaccatcc ctcactggta tcgcatgatt aaccgtctga tgtggatctg
     1561 gcgcggcatt gacccacgcg aaatcctcga cgtccaggca cgtattgtga tgagcgatgc
     1621 cgaacgtacc gacgatgatt tatacgatac ggtgattggc taccgtggcg gcaactggat
     1681 ttatgagtgg gccacccagg cgatggtgtg gcaacaaaaa gcctgtgcgg aagaagatcc
     1741 gcaactcagt ggtcgtcact ggctgcatgc ggctacgttg tacaacattg ccgcctatcc
     1801 tcatctgaaa ggagatgacc tggccgagca agcgcaggct ttgtcaaacc gcgcctatga
     1861 agaggccgct cagcgtctac cgggcacgat gcgacagatg gagtttaccg tacccggcgg
     1921 tgcgcccatc accggctttt tgcatatgcc gaaaggcgat ggcccgttcc cg
//
LOCUS       DAZFAB010000478         1340 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.guided.298, whole genome shotgun sequence.
ACCESSION   DAZFAB010000478 DAZFAB010000000
VERSION     DAZFAB010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.guided.298"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            42..311
                     /locus_tag="ACHJWA_005327"
     CDS             42..311
                     /locus_tag="ACHJWA_005327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2c subunit B"
                     /protein_id="HFO4311704.1"
                     /translation="MKKMFMAVLFALVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
     gene            complement(608..931)
                     /locus_tag="ACHJWA_005328"
     CDS             complement(608..931)
                     /locus_tag="ACHJWA_005328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFO4311705.1"
                     /translation="MNWIVIDTVIQPACGISFSAIWCNIKLILWYQSDTFLPPGCIFT
                     PTRTGVILNNKELPVTIYNVAPFNKNVWNLIKNSQECPANTGNITDKCFNHRCILEIC
                     PYGRK"
     gene            1175..>1340
                     /locus_tag="ACHJWA_005329"
     CDS             1175..>1340
                     /locus_tag="ACHJWA_005329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO4311706.1"
                     /translation="MTFKHYDVVRAASPSDLAERLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTT"
BASE COUNT          350 a          246 c          335 g          409 t
ORIGIN      
        1 tcacagtttt tatatacaac gggtaaataa aggagttaag tatgaagaag atgtttatgg
       61 cggttttatt tgcattagtt tctgttaatg caatggcggc ggattgcgct aaaggtaaaa
      121 ttgagttttc caagtataat gagaatgata cattcacagt aaaagtggcc ggaaaagagt
      181 actggaccag tcgctggaat ctgcaaccgt tactgcaaag tgctcagttg acaggaatga
      241 ctgtcacaat caaatccagt acctgtgaat caggctccgg atttgctgaa gtgcagttta
      301 ataatgactg aggcataacc tgattcgtgg tatgtgggta acaagtgtaa tctgtgtcac
      361 aattcagtca gttgacagtt gcctgtcaga ctgagcattc gttaaaaaaa ttcgcatggt
      421 gaatccccct gtgcggaggg gcgactggtg tataaggtgt catctctgac gacaacctgg
      481 agcgagatac gcgggttcag ggacaccgga ctgaactcac cgggaggcac ccggcaccat
      541 gtacatgatg atacagatac gcagtgtcag cccctctccg gaggggcttt ttatctgaat
      601 gattctgtta ttttcgcccg tatgggcata tttccagaat gcagcgatga ttaaaacact
      661 tatctgtgat atttcctgtg tttgcagggc actcctggct gtttttaatt aaattccaga
      721 cgtttttatt aaatggggcc acgttgtaaa tggtgacggg gagctcttta ttattgagta
      781 ttacgcctgt ccgggttggt gtaaaaatac agcccggagg gaggaaggta tctgactgat
      841 accataatat taattttatg ttacaccata tggctgaaaa tgatatgcca catgctggct
      901 ggattaccgt gtcaatcact atccagttca tttgctttcc ttattttctt aaggttttaa
      961 atgatgccat aaattatagg tgatgttttg aaagcaatct gtcaggctga gttgtttttc
     1021 tggtgtcgtt tattctttgt ttaaaataag agggcatttt aaattgtcgg cgtttttttg
     1081 ttatataaga gacatggttt ttttatagcg tgttttttat gtgttacatg attgtgtgtt
     1141 ccggataatt actttgctgg cggcaggaga ggttatgaca tttaaacact acgatgtggt
     1201 cagggcggca tcgccgtcag accttgctga acgactgaca caaaaactga aggagggctg
     1261 gcagccattt ggcagtccgg tggccatcac gccttacacc ctgatgcagg ccattgcggc
     1321 ggaaggtgat gtcaccacac
//
LOCUS       DAZFAB010000479         1064 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2302F2984
            SAMN43283636-rid23063473.guided.332, whole genome shotgun sequence.
ACCESSION   DAZFAB010000479 DAZFAB010000000
VERSION     DAZFAB010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283636
            Sequence Read Archive: SRR30325276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351538.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351538.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:03:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 92
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 100 of 227
            Pseudo Genes (incomplete)         :: 118 of 227
            Pseudo Genes (internal stop)      :: 59 of 227
            Pseudo Genes (multiple problems)  :: 39 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283636-rid23063473.guided.332"
                     /serotype="O157:H 7"
                     /isolate="2302F2984"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            95..1054
                     /locus_tag="ACHJWA_005330"
     CDS             95..1054
                     /locus_tag="ACHJWA_005330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HFO4311707.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     DSQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
BASE COUNT          280 a          225 c          254 g          305 t
ORIGIN      
        1 cacacttatt ttccctggct cgcttttgcg ggcctttttt atatctgcgc cgggtctggt
       61 gctgattact tcagccaaaa ggaacacctt gtatatgaag tgtatattat ttaaatgggt
      121 actgtgcctg ttactgggct tttcttcggt atcctattcc cgggaattta cgatagactt
      181 ttcgactcaa caaagttatg tatcttcgtt aaatagtata cggacagaga tatcgacccc
      241 tcttgaacat atatctcagg ggaccacatc ggtgtctgtt attaaccaca ccccaccggg
      301 cagttatttt gctgtggata tacgagggct tgatgtctat caggcgcgtt ttgaccatct
      361 tcgtctgatt attgagcaaa ataatttata tgtggccggg ttcgttaata cggcaacaaa
      421 tactttctac cgtttttcag attttacaca tatatcagtg cccggtgtga caacggtttc
      481 catgacaacg gacagcagtt ataccactct gcaacgtgtc gcagcgctgg aacgttccgg
      541 aatgcaaatc agtcgtcact cactggtttc atcatatctg gcgttaatgg agttcagtgg
      601 taatacaatg accagagatg catccagagc agttctgcgt tttgtcactg tcacagcaga
      661 agccttacgc ttcaggcaga tacagagaga atttcgtcag gcactgtctg aaactgctcc
      721 tgtgtatacg atgacgccgg gagacgtgga cctcactctg aactgggggc gaatcagcaa
      781 tgtgcttccg gagtatcggg gagaggatgg tgtcagagtg gggagaatat cctttaataa
      841 tatatcggcg atactgggca ctgtggccgt tatactgaat tgtcatcatc agggggcgcg
      901 ttctgttcgc gccgtgaatg aagatagtca accagaatgt cagataactg gcgacaggcc
      961 cgttataaaa ataaacaata cattatggga aagtaataca gctgcagcgt ttctgaacag
     1021 aaagtcacag tttttatata caacgggtaa ataaaggagt taag
//
